LOCUS       DAOSOA010000001        59807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_555_length_59807_cov_7.486360, whole genome shotgun sequence.
ACCESSION   DAOSOA010000001 DAOSOA010000000
VERSION     DAOSOA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 59807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59807
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_555_length_59807_cov_7.486360"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PKY13_00005"
     CDS             complement(<1..737)
                     /locus_tag="PKY13_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78330.1"
                     /translation="MTTPYVQELSRVEVGRSESWTDAKRHYYWLGLIVPVLPLLAAGL
                     VAATGAGWGWWLGPVVLYVLMPILDVTLGRDHENPPEELVPQLNDDHFYRWCTYAYLP
                     LQFLSLVVACAYWAWGDLSTIDALGLAATVGSVGGVAINTAHELGHKHPKVEQWLAKV
                     SLAQTGYGHFFVEHNRGHHVHVSTPEDPASSRLGQSLYSFLPRTVFGSVRSAVRIERD
                     RRRRAGDRFFSIHNDILNAWAMSVVLFG"
     gene            934..1004
                     /locus_tag="PKY13_00010"
     tRNA            934..1004
                     /locus_tag="PKY13_00010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Gly,seq:ccc)
     gene            1333..2520
                     /locus_tag="PKY13_00015"
     CDS             1333..2520
                     /locus_tag="PKY13_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HOT78331.1"
                     /translation="MSLATSPVGSLVPISGYDARFGEDYHADAFVPTDLPDCVDLPGA
                     VWMAISDANAELGRLDAAASLVPNPQLVTRIATRREAVGTSALEGTYAELTELYAAEV
                     LPFDEQDTEVAPNVREVMNYARAADAAYGWIGERPITLQMLASLQAEIVRGTESDSQE
                     AGAIRESQVFIGAKHRRVTEARFIPPPPGDQLRAMCERWVEWLSAPAPLAQIQLIPRI
                     AMAHYQFETLHPFIDGNGRVGRLVAILQLMSEHALRSPVLSVSPWLKDRADEYRDHLL
                     AVSMTGDWAPWVEFFANAVAVEARSGHERIMRLLELREQLGETVRSALPRARLALEIT
                     DDLIAFPILSVSVAQRRYGRSNQANRDAIGSLVELGVLEPYGEARYDRLFWNRRVFQI
                     IEG"
     gene            3227..3520
                     /locus_tag="PKY13_00020"
     CDS             3227..3520
                     /locus_tag="PKY13_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT78332.1"
                     /translation="MANDDPSNASDIVHLAGDYDIARQDELRSELLSSDADVVVADLR
                     DVSFLDSSAIHAFVDARNQLTARGRDIQLINLTGMPRRVFDITGLTPYFTIAE"
     gene            complement(3539..4033)
                     /locus_tag="PKY13_00025"
     CDS             complement(3539..4033)
                     /locus_tag="PKY13_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT78333.1"
                     /translation="MGIELTPIGTVVGGRVVPEDDGWDAETCEIRLDPERFEASALVG
                     LADFSHVEVVYHFHLVDEADVVASARRPRGRADWPEVGIFAQRGRVRPNRLGVSVARL
                     VGIEGLAVRVQGLDAVDGTPVLDLKPVLSGFLPRGPVVEPSWSLELMAGYWGGDHLAN
                     PAGG"
     gene            complement(4035..5273)
                     /locus_tag="PKY13_00030"
     CDS             complement(4035..5273)
                     /locus_tag="PKY13_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HOT78334.1"
                     /translation="MPTAPNVIVVGGGPGGLMAAEQLAHSGSSVTVFEQMPSVGRKFL
                     LAGRGGLNLTHSEPTEQLVERYGSASSRVGPAVARFDAAALRAWAESLGEPTFVGSSG
                     RVFPESFRATPLLRAWLARLASLGVVIRTRHRWLGVELRHDGRLEVSVADQDGVESVV
                     VADAVVLAMGGASWPKVGSDGAWTSALSAAGVGLAPFRPANCGFVSAWTEVFRRRFAG
                     APLKNVELSAGGRSVRGEGVVTETGVEGGVFYALGSTIRDLIELEGEVVVHLDLRPDL
                     SAEQVAERLARRRPKDSATTAFRKAGITPVGIGLLREATANELPSDPVDLAALVKATP
                     LRLVGAEAVERSISTAGGVCLDEVDERFMLRRLPGVFVVGEMLDWEAPTGGYLLQATF
                     STAVAAAAGVLDHLGHRNGG"
     gene            complement(5330..6733)
                     /locus_tag="PKY13_00035"
     CDS             complement(5330..6733)
                     /locus_tag="PKY13_00035"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HOT78335.1"
                     /translation="MRTTAIPDLAAPPVVRSRRGLAAALVERGLRPVPASSLAAFRVA
                     AGLSAAVVAARFVSRGWVERLYLAPSYHFTYPGLSWVRPLPAAGMYALYAVMVLAGLA
                     LALGWRPRWAALAFTLGFAYAEAIDVTTYLNHYELVTLIGVLCVVLPLPGRGSAVRST
                     VPAWAVWLLRFQLGAVYVFAGLGKVDVDWLLRGEPLHTWLLGRTDLPLVGPWFADRWV
                     AVALSWGGALFDLGVVAFLLRRRTRPYAYAAVVVFHLITGWLFPGIGAFPFVMIVLTP
                     IFFEPDWPDRVRGAVGRRFGLVSVPPVTPVAAAAVTPSSAWRVRLGTAAVVVWVVVQL
                     VVPLRHLAYPGDVRWTEEGYRWSWRVLLTEKEGSATFRVTDPATGLTRTVWPSDELAP
                     HQVRSMSVRPDLIRQYAHHLADETERSGAARPEVRVDAWVAIAGLPRTRLMDPAVDLA
                     AEPLDLWPADDIEPSPL"
     gene            complement(6711..7724)
                     /locus_tag="PKY13_00040"
     CDS             complement(6711..7724)
                     /locus_tag="PKY13_00040"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HOT78336.1"
                     /translation="MLFVTTLVAGCSSDDEPTKAAPATADRAEVVAALVQDVILPANA
                     SAATEAAAASTAIGELCESPDSTRLEVARSAVATAWGAWRATDSFDVGPAMQRRSSSV
                     VSYKVDVAKVDATLAGSPPTDPETVRNRTSSSLRGYGAAWHLLTADAGAFSAVPARCA
                     YLSAVMSVIADETATVDDGWTTGLDGAAPYSDTATGVGDDALSPTDMIDSLVNMQLSQ
                     LESTSKLLTAESELAPGADSDLPVGEMFQFAAQVRGIGDSYTELDALLKPELSKAISD
                     EVDALARILADDATEPSETPPTADRAKAVATAVEELRVTIATEVVSALDVTVSFSEND
                     GDS"
     gene            complement(7775..9187)
                     /locus_tag="PKY13_00045"
     CDS             complement(7775..9187)
                     /locus_tag="PKY13_00045"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HOT78337.1"
                     /translation="MKRAVPIAIVAAATIAMGLAGCSNERAARPAEVALADLLDSDLG
                     GDTTRNVVNQNAFGLPAKSLPGSSRADFEVGDSFFTQNWVIAPASTTSRDGLGPTFNA
                     TACASCHTFDGRAEPPAEGGPATPGLLLRLSIAGKAAHGAPKAHPTYGGQLQDRSVPG
                     VPAEGSIAITYEDIEGAFADGTTYTLRKPVYEIGDPAFGEPGEILIGPRIAPQIPGMG
                     LLEAVPAATIERAADPDDADGDGISGRTNTVWDEVAGTTAIGRFGWKANVASVRQQTA
                     GAFSGDVGITSPVFPDADCPEAQTACAAAPSGGSPEIDDDTFDAVVFYTRVLSVPAAR
                     DADDKNVRRGAQVFADSGCASCHTPKLTTGASDIDALANQTIHPFTDLLLHDMGPDLA
                     DGRPDFEASGSEWRTPPLWGIGLVETINGHTRFLHDGRARNLLETILWHGGEGEASRD
                     AVLDLPKKDRDALIDFLESR"
     gene            complement(9187..10362)
                     /locus_tag="PKY13_00050"
     CDS             complement(9187..10362)
                     /locus_tag="PKY13_00050"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HOT78338.1"
                     /translation="MIALAGLTLSACSGDDSATDDEPTTTAASGKLNEALAEEVVAHY
                     AEGVFASYTATLDGAKALDAAVEAFVADPTEATLTTARSAWIAARTPYGLTEAFRFYG
                     GPIDNKEDGPEGRINGWPLDEQYIDSTVDTPDSGIIADTVGFPEITKDALAAANEQGG
                     EANLSTGWHAIEFLLWGQDQSDTGPGARPATDYTTAANAERRKLYLSAVTDLLIDDLT
                     QVTDQWAPDADNYRSEFVARPVTETLSDMITGIGELSRGELAGERMNVAFEERDQENE
                     HSCFSDNTTADLSANANGIANVWLGSYAGVASGPGLASLVADADAALSSDITAQISES
                     VLEISKIPAPFDQNLTADAPDDGPGRTAIRNAIEDLGTQTDSIVAAGKALGVTVEVS"
     gene            complement(10622..11605)
                     /locus_tag="PKY13_00055"
     CDS             complement(10622..11605)
                     /locus_tag="PKY13_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78339.1"
                     /translation="MTTTRPTRHRRRSTALLGVILGATALVATACPEPPPSAGSGTTT
                     TPGPTTTAAPTTTTPSSIKTLTNASFEWTVSKQTNNASQAPGQVNYWSAGISDGTAAT
                     YTATNGNATVLKKNAAGTYVPIGSETAVSFANKNRDGAGNVVTSSNAFFLGQKVRYTG
                     GTGTVNTATGASTIQWTGTFTINFYGTYVPFWIKNPKLVVNGSGVGTLTASIAGFASS
                     QDDPTIRIQLPERTVTLAEFTGLSAANTSGFTGTPAFLNVAAQLPSAHATAAPQIAKT
                     SANAGYWGAWPKSFVDFQIETGLGAYWYTSGSSSDVNKPTDPLTVAYNIAP"
     gene            complement(11693..12796)
                     /locus_tag="PKY13_00060"
     CDS             complement(11693..12796)
                     /locus_tag="PKY13_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78340.1"
                     /translation="MNARTLLAAAAGAVLSVAAVAGLGVAPAGAQTPTLTVSQSVLPA
                     AQATTVTVVGTSYLVPPHSSDKNVFGGVYVMFGWVQPGSTWGPSSRNGANSNGQFGIT
                     YSYAGVNRGADTRDDGSGLNRFVAFTQGEVNDGTTAFAMSMDPAFPDNSRGNWTTTIT
                     VPGSTYQWVDPATNMTNTVDCLQVQCGIYTIGAHGKASATNERFTPISFSTGAGAPVT
                     IPPNASSNPGGSTGPGQTGAGQTATTAPSVVGGSSTGRTPTTAKKSGPATTSPGSTAP
                     VDTSPVEPTTTMTTETTAAETTTTTTTAVESSTTTRRRSATDETNSASGRVIVFESDD
                     DGGMSGWVVGGAVGVPLVAAGGVVVWRRRAAGV"
     assembly_gap    12869..12968
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12969..13696)
                     /locus_tag="PKY13_00065"
     CDS             complement(<12969..13696)
                     /locus_tag="PKY13_00065"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HOT78341.1"
                     /translation="MSWRRIVRIPAVARRGDVVLSARGIGVSFGDREILAGVDLDVRA
                     GEVVALVGPNGAGKSTLLSVLAGDREPSAGTVLVSGAPVAAWTGIELAMRRGVLPQRS
                     DVSFPFTVEQIVRMGREPWRNTDREDADDDVYDWAVRVTDVSALTRRDFPSLSGGEQA
                     RSALARVLAQEPGLLLLDEPTASLDISHQEQVLGIARDRAGVGDGVVVVLHDLGLAAA
                     YADRVVVIHDGGVAADGRPEAVMTG"
     gene            complement(13693..14778)
                     /locus_tag="PKY13_00070"
     CDS             complement(13693..14778)
                     /locus_tag="PKY13_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT78342.1"
                     /translation="MATATVPVVTDSASTVHSRSRAAVVIGVLGLALVAAVLVAAGTG
                     QLHVAPGEVLGSVFHRIGLDWGAMPAHSNGDAVLWDVRFPRVIMAVLVGAALGVAGAL
                     MQGVFGNPLAEPAVVGVSSGAAVGACAVIVWKVTVLGTFTTPVAAFMASLITTLLVYG
                     LSRSNGRTEVVTIVLMGIAVNAVAAGLIGFFVFLGDTQAREQIVFWQLGSLNGIRWQA
                     VAAVSPLIVVGLVGAISVARRLDLLALGERPARHLGVNVERLRITTIVMVALLVGPAV
                     AFAGIIGFVGLVVAHLLRMLIGPSHPRLIPACALGGALVLVVADLFARTAVDFADLPI
                     GMLTALLGGPSFFWLLRRERNRAGGWA"
     gene            complement(14778..15851)
                     /locus_tag="PKY13_00075"
     CDS             complement(14778..15851)
                     /locus_tag="PKY13_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT78343.1"
                     /translation="MPTILRTQRASMVAIAAAVASAVVLSACGGSGAPVDRSAADPTA
                     SGCVGARTATSQEWVRPLEPAPSPNLPVTVTDVTGQSITISETSRILALDTYGTLGTT
                     VYALGLGDRLVGRDVSTGVPALVDLPVVTANGHELQAEAILDLDPSVILTDYSIGPLE
                     VQMQLKDAGIPVVILDDHRARDTVGEQIRAVAHALGVDAVGDAVASRVEREISAAETQ
                     VSEMANASPLKGMRMVFLYLRGSAGVFYWFGEGSGADDLIEALGGVDVATQVELDGYK
                     PINAEGLVTAAPDMFLLMTGGLESVGGVSGLAEIPGVAETRAGTNECVVDMADSQILS
                     FGPQYPATLSALAEAVYGTPAGD"
     gene            15989..17035
                     /locus_tag="PKY13_00080"
     CDS             15989..17035
                     /locus_tag="PKY13_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOT78344.1"
                     /translation="MTDTDTSRTTDPVLQEAPLGFQWPTIDPFLFCAHHRDDYPAGND
                     ALGPDAPLVGRSIGQDFDGVDGWNMYHGDVVPGFPQHPHRGFETVTFVRSGLIDHSDS
                     LGAAARFGRGDVQWMTAGRGVVHSEMFPLLDRDGPNPAELFQIWLNLPAEDKLVDPYF
                     TMLWNHDIPRIATSGDDGATATVTVIAGRLEGVTAPPPPPNSWAARSEAEIGIWHISL
                     DPGASWELPPTAGEDTVRTLYLFEGSELTIGGHVIGASTGALLASDAPVRLTATGTAV
                     EALVLQGRPIGQPVARYGPFVMNTEAEIEQAFADYRETGFGGWPWDVDAPVHGRHQGR
                     FARHADGRREDIGD"
     gene            17234..18502
                     /locus_tag="PKY13_00085"
     CDS             17234..18502
                     /locus_tag="PKY13_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT78345.1"
                     /translation="MGTPATETDVVVIGGGLAGLTAAAFAARAGATVRVLDARTDLGG
                     RARTAVHDGYHFNEGPHALYTASDGARALRELGISPKGGTPPLLRAKISLDGRLRVAP
                     PRRALAQFVGLLRRVGTDRNDPGLVRISAREWIEARIADPVGRHVAAGAVRVSSYSGD
                     LSTFSADAAATQLHAALRGVTYLHGGWAQLVDATKAAATAAGATVEHTSKVTSIDTDG
                     ERHLVALGDGRRLITRAVVLAAGGPSTATRLVGDRSPTLTAADLAAIPVHAACLDLGL
                     RRLTTSSTRFVLGIDRPTYASIHTPGAHLADDGHVAHVMYYEPGDDIGVADLEALADE
                     LQPGWRDEEVARQIGHRRVVAYDRPQPGIGLRGRPGPIVADLPGVFVAGDWVGPTDLL
                     GTAAITSGKAAGIAAATHRSAFSTVPASAR"
     gene            18499..19416
                     /gene="sigJ"
                     /locus_tag="PKY13_00090"
     CDS             18499..19416
                     /gene="sigJ"
                     /locus_tag="PKY13_00090"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HOT78346.1"
                     /translation="MSATPDVAAERFEPHRDRLFGVSYRMTGSVADAEDCVQEAFVRW
                     QRTDIGAVDHDEAFLVRTVSRLAIDRQRQAARRRETYVGPWLPEPLLAPVTQLATASV
                     DPADEAELADSLSFAFVVMLDRLSASERAAFILHDVFGVPFDEIATTLEREPEACRQL
                     ASRARRKLRDTDASADAGPRPAVGTDAANALGALLGALLTGDVEACLHHLAPGVVLTS
                     DAGPHRRAARRHVVGADRVVRLVTNLFARDVGLVTEIRPVAVNGAMGLYVDHVEGPLV
                     FTGDSGPDGRIHHIWIQMNPEKVSSPPDR"
     gene            19498..20586
                     /locus_tag="PKY13_00095"
     CDS             19498..20586
                     /locus_tag="PKY13_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78347.1"
                     /translation="MAIGVVAAAVAAGAGCAPDIGTLTPQQQVDQLIAFVESARGHQF
                     VTRPDVEFVPDAVFQQHVLDSIEGAEAALDVDEVAFKALGWMPTTDDLFEKYKIAFSG
                     TVVGFYDPASKVLEVRGDQLTPYRREVVVHELTHALDDQLFDLNESMGDGVLSEQQLA
                     FLVGVEGDAVRTQQAYVATMTPLEQLQNVAEQLSFQIDPAILSVPLALLSLSQTPYLQ
                     GPQFVAGLGGNAGIDAMFDRYPATAEQAWNPAKYLANEGAVAVPTPPAGGSIVNSGSW
                     GQFFMTLLLGEGVNLDATLNPATDGWAGDSFVTWTSGTASCIRIDTRSDTAPQSDALR
                     GALTTWASSHGAIVSAVDATTARVTACN"
     gene            20716..21054
                     /locus_tag="PKY13_00100"
     CDS             20716..21054
                     /locus_tag="PKY13_00100"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT78348.1"
                     /translation="MGGPERRWPRQGGEPARFAAAADLLKALASPHRVAIVDLLAAGP
                     LRVHEIVEALGVDQPLVSQHLRVLRDADLLTSERHGKEVVYSLADEHIAHIVGDAITH
                     AAEVPGGESS"
     gene            21097..22074
                     /locus_tag="PKY13_00105"
     CDS             21097..22074
                     /locus_tag="PKY13_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78349.1"
                     /translation="MQIVYLSNRPQVMRETWQHVRHFMPWIDRALIVAPAARHGEFDA
                     WVDPTEITLFSDEEVSGATSSTLGVLDHVRRNVRLRRALIGSGATDDVFLLSDDDYRP
                     MRPVEESFFTDGSRDIGYFSYELGEWPGYETPFDEAQHVTHDALAFLGLPHLAYGAHM
                     PQIMRRDLWGEAFELFETVSDDDMVCEWALYFNVAQARHPERFDAPRPFEVMGWPQYG
                     AEWPWWVRPPRWTFENFYPDMYEPGHLFDGLATGLDADGVTKTNFDKITRWTAFARAA
                     ERLDFPAGVHNPWTKDSALRRAYFAALRPARKTLRYLTETRGSGTRDAD"
     gene            22125..23657
                     /locus_tag="PKY13_00110"
     CDS             22125..23657
                     /locus_tag="PKY13_00110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT78350.1"
                     /translation="MLSFARRPVVALLALAVAASVLGRFFVRSPLWLDEALSVNISKL
                     PIGDIAGALKHDGHPPLYYYLLHGWMEVFGDGDRAVRALSGLITLGTLPLAFLVGRRI
                     AGRRLGLTLAVVFALSPYAFRYGSETRMYALLMAEVFAGYLLLDAAITAPRWKTLVGI
                     STVSGLLLWTHYWSMWLLGAIGVLALVRLARAQRRGDRPLVVGTAKIVASLAVGGLTF
                     LPWLPTLLYQSQHTGTPWAPSFRVTTLVVTSITEFAGGPYSEAQLGVLLLTILVAIGV
                     FGTGIDNQRIELDFFAHPLARRPLSVLALTIAIAAAVGMVNGMAFSPRYAAVFFPFFL
                     ILVALGLQQFKGGLVRDVVLVTFTALSLAGMFLVVRVDRTQARASVEVIERTAPDGVV
                     IACPDQLGPSVSRYLDRERYDITTYPRFASPELVDWVDYKERNERNDPAAFAQELLRR
                     AEGRPLFLVYRDDFLTLKGQCQRVIETLSAVRPPRTIQRESGDDFYEPMTVVEFGQPG
                     AG"
     gene            complement(23686..24804)
                     /locus_tag="PKY13_00115"
     CDS             complement(23686..24804)
                     /locus_tag="PKY13_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78351.1"
                     /translation="MERPLDRVAASPYLTALAAWVTARVLLAVAWAASSMSWFDRFET
                     ATSVRHQGLFLWDGTYYRDLAQHGYDGVATEVQRFFPLFPLLGRWVGWLLGGNDDLGL
                     LVVSNLAALAALVLVYVLVQDVTGDRRLAVASVWWLALFPAASVLVFAYSESLALALA
                     LGAVLAVRRERWAPAIVLGVLSGLTRPTGALVAIALFVAVLDDLRRRRGVVVHLAAVV
                     APGVGTVGYLAWLQWRFDSWRVPLDSQRVFRAGWHEPVSRLVTAVVDVAGGQFRDVFN
                     ASFAVGAIVVIILAARRLPVGWTIYTAVTLVIALAANNINSLGRYAFTAFPLAVMVAV
                     AQQRLPARWRWLDRLLLPMSGVLMAGYAVAAWSGRMIP"
     gene            complement(24818..25546)
                     /locus_tag="PKY13_00120"
     CDS             complement(24818..25546)
                     /locus_tag="PKY13_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78352.1"
                     /translation="MSAAPILEVTGLCAGYDGRTVLHDLDLTVATGEVVCLLGANGAG
                     KSTALGAMTGLVRATSGTVALDGEPLPLSSTHRIARAGVAHVPEDRSLFPSLTVRDHL
                     RLADSRRARRRADEVDAVARVLDWFPALERLLSRRVGLLSGGEQQMVAIGRAIMSRPR
                     VLLIDEMSLGLAPLVVASLLDVIGRLAADTGCGVLLVEQHVALALGVASRATVLSRGR
                     VTFEGPADELAASHDTLVASYLGD"
     gene            complement(25543..26268)
                     /locus_tag="PKY13_00125"
     CDS             complement(25543..26268)
                     /locus_tag="PKY13_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT78353.1"
                     /translation="MSVRYGSNVVVVGADLDVGAGELVGLIGANGAGKTTTIDAVSGF
                     VRHGGTVHLDGTDLSSLPPHERARAGIGRTWQGIELFDDLTVRQNCLVAARRPGWKGV
                     LHDLLHRPDDAATDARVDDALAAVGLAADAARRPSELSHGHQKLAGVARALAGGPRVL
                     LLDEPAAGLDQTESLAFGVTLRRLVDESGIGALLIDHDTRLIFEVCDRVYVLDFGAIV
                     AAGTAAEVRNDPKVIEAYLGVAT"
     gene            complement(26289..26525)
                     /locus_tag="PKY13_00130"
     CDS             complement(26289..26525)
                     /locus_tag="PKY13_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78354.1"
                     /translation="MISAEQLFLGNDLLAWLMLAVGGALAVANVAAVVRPPREDPADP
                     TSPRREAAPISRAAPLVLLGVLVSAWALASLLKG"
     gene            26828..>27855
                     /locus_tag="PKY13_00135"
     CDS             26828..>27855
                     /locus_tag="PKY13_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOT78355.1"
                     /translation="MAESTTSTDTHGSPAARTKDRPWLMRTYSGHSTAKASNELYRTN
                     LAKGQTGLSIAFDLPTQTGYDPDHVMARGEVGKVGVPVAHLGHMRQLLDGIPQGEMNT
                     SMTINATAAWMLGLYVANAEDHGVDSKVLRGTTQNDIVKEYLSRGTYIFPPEASRRLI
                     VDMFAYCNKNVPHWNPMNVCSYHLQEAGATPTQEIAYSLACAIGVLDAVRDSGQVAPD
                     AMGEVVASVSFFVNAGIRFIEENCKMRAFTQMWDRLCEERYGVTDPKARRFRYGVQVN
                     SLGLTEAQPENNIQRIVLEALGVTLSKSARARSLQLPAWNEALGLPRPWDQQWSLRIM
                     QVLAFETDL"
     assembly_gap    27856..27955
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27956..28986
                     /locus_tag="PKY13_00140"
     CDS             <27956..28986
                     /locus_tag="PKY13_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOT78356.1"
                     /translation="DQQWSLRIMQVLAFETDLLEYGDIFEGSKVIEGLTAELVDSAQA
                     ELDDILALGGAFEAIDEMKGRLVRSHTERMRRIESGEQMVIGVNAFTETAESPLGGED
                     NILKVDPAVQAAAIEELAEWKANRDQAAVDAALDELERVARTDENIMAASIALAHAGG
                     TTGEWAGRLREVFGEFRAPTGVGAATGAGGASTGLRELADRLKDTPGGPPRFLVAKPG
                     LDGHSNGAEQIAVAGRDAGMEVIYQGIRLTPEQIAAAARDEDVDIVGLSILSGSHLEL
                     VPEVVRLLRAEGVTCPVVAGGIIPEDDRPRLLEAGVAAVFTPKDFELTRLMTTVADLA
                     LEHRSVSA"
     gene            29066..30892
                     /locus_tag="PKY13_00145"
     CDS             29066..30892
                     /locus_tag="PKY13_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT78357.1"
                     /translation="MTPDRATAPRPAGGTHRAAAVALVASMALLAGCGGSAGKITLNR
                     GDSSDTAPEVSTGPDGTAPTTGSLTWGSCSKFDIAVTADVLSSARQSGLECAALDVPV
                     DHAAPDGATTELALARLPATGSSSKRIGSLIVNPGGPGGSGLEFLASSGLAIPDDLRQ
                     RFDIVSFDPRGIASSSPLDCLTQTQRADLVDAESPDDPAQAQAQAADTERTIAEGCEA
                     DDAELFRHMGTDQVAGDLDDIREAVGDDELTFLGLSYGTRIAASYATQFPDKVRAIVL
                     DGSVTPSRDLAESSGGQIKGIVRALDNFVQLCNADTTCAIAPDALGKLTAVADSLDAT
                     PMTIDGGDGEETLDKDKYLTGVVTALYDPSVSMALADAVAALLGTDSDRAQQAGRFLL
                     DLAGQQDSKQPDGTYGNGFETQGVVNCLDAADPLDASELPLVREIAGPIPPLLDSDPA
                     TDTPSCTLLPTGDRLEIAANDAKDRLLIVGTEGDPATPIEWTRAMTAALGDPAVIVYG
                     GSGHTASLSRKCVTSQVTEFFISGKVPASLQDCPRDPAESDIYAQIGNQFETMGLGAD
                     IGTCIANALRGEIDPLGIISLNGDDPDPEVISKLQRAALSCR"
     gene            30963..31817
                     /locus_tag="PKY13_00150"
     CDS             30963..31817
                     /locus_tag="PKY13_00150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT78358.1"
                     /translation="MGLRRLTGILLGCVGLALGIAGLVADLPVLGLVGGIAAVLAGLV
                     AFGAAATLGDAEARATTAETETATLRRELATATQNLNEERTQHDATRQSLADYMADQP
                     AAGSFDRKLSDTEAPLGQRSTTPSPTDTRLLADPETNLFSEAYFRVALDARIASARRH
                     LRPVAVALVEVVEGSGAPGSPEADATKVTGSIRQTLRDADTACRMADGCFALILEDTP
                     ENGAVWTVERIRRNLTSRFGQHTMWAGVACYPAHAFSTDELLDQASLALESAREWKQD
                     RIEVATAE"
     gene            complement(31854..32519)
                     /locus_tag="PKY13_00155"
     CDS             complement(31854..32519)
                     /locus_tag="PKY13_00155"
                     /inference="COORDINATES: protein motif:HMM:NF037005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT78359.1"
                     /translation="MANGTAPLTRHDLDLTPRRRPVQERSRERFARILRATRALLLET
                     GIEALSCESIAAAADVPVGTVYQFFPNKFSIVCELDRLDTESVAAELDAFGAQIPSLE
                     WKELLDALIDHLAMEWSHDPSRRAVWLSMQATPATREASTRHQQILGDRVARIISPLT
                     PGTHRRERSTIAQVVVHTCYSMLNFSVRDHRAHPAAVRETKRLLTAYLEVAAAESLAT
                     QGS"
     gene            complement(32576..33214)
                     /locus_tag="PKY13_00160"
     CDS             complement(32576..33214)
                     /locus_tag="PKY13_00160"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOT78360.1"
                     /translation="MTALVAPLPYDEIVELLAEASAAFVDTVVGITSDEWSEPGLGVW
                     NVRELVGHTMRAFSTVHRFLDEPVDRVEVETAPDYFRIALGSSPGIHDQVADRGREAG
                     VLLGDDPVASVVEDVSRLLGRLGEMTGDEIGVTVVGGMLLPAYLDTRLVELVVHLSDL
                     CVALGRPAPDLGRAGVRVAALVFASASVEDRDTVVRAMLGRADLPPGFNVWP"
     gene            complement(33211..34233)
                     /locus_tag="PKY13_00165"
     CDS             complement(33211..34233)
                     /locus_tag="PKY13_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT78361.1"
                     /translation="MEITTVSTDEIVLFDGRELRTYADLEPGRVYEFGDQHVRTLVEP
                     GPFLGRVATVNDVHFGETECGVISGTDIGPTFSTAPGEEPYPEVMNRGAIAELSRIDP
                     EVVVVKGDLTSTGTHDEYERFLDAYGSAFGDRLVHVRGNHDSYHGQVFADFPMQEVVV
                     EGATLAVLDTARLGQVNGSISADQIDWLDELASRTDTQVLVFGHHHVWNPELDPRRDD
                     FFGIRPADSEALFEVFARRHNLTGYLAGHTHRNRRMHVAEAGGAPFVEVACVKDFPGA
                     VAEYRIHDGGIVQVFRRISEPEALRWSERTRQMYEGGYGAYAFGRLADRCFTMADRRA
                     RTAVAT"
     gene            34397..34690
                     /locus_tag="PKY13_00170"
     CDS             34397..34690
                     /locus_tag="PKY13_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78362.1"
                     /translation="METILEAIARIRSAGYDLEMSATPEGWLQCGVCNEVVDPATAVV
                     DETVRFEGESNPDDEAIVVAISTPHGHQGYIVAAYGADMEAGDVALLQALTAR"
     gene            complement(34704..37226)
                     /locus_tag="PKY13_00175"
     CDS             complement(34704..37226)
                     /locus_tag="PKY13_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT78363.1"
                     /translation="MSLDPNTWTLKTQEAVNAAIELASTSDNPEVTPDHLLVTLISQD
                     NGVVLPVIRKIGLNPLSLRNRTEESLARLPKAYGTDTSISKELTGIFQEATKQRGELK
                     DDYLSTEHLLLALVIADEQRSPAEKRLDGTTKEDVLAALAEVRGSHRVTSQNPEEQYQ
                     SLEKYGRDLTEAARRGKLDPVIGRDEEIRRVVQVLSRRTKNNPVLIGEPGVGKTAVVE
                     GLAQRIIEGDVPESLRNKRLIALDLASMVAGAKYRGEFEERLKAVLKEITDSNGEIIT
                     FIDEMHTVVGAGGGSEGAMDAGNMLKPMLARGELRMVGATTLDEFRKYIEKDPALERR
                     FQQVYVGEPTVEDSIAILRGLKERYEVHHGVRIEDNALIAAAVLSDRYLTGRFLPDKA
                     IDLMDEAASALRIEIDSVPTEIDVVERRIRGLDMERVALSKESDPVSLERLLAIDKEL
                     ADLREQADSMRGHWQGEKDAIAIIQELKERLETMKADADRAEREGDLTRASEIRYGHL
                     PVIEREVEAATAQLAELQTDHQMLKEEVDESDIAKVVAKWTGIPVTRLIEGEMGKLLR
                     LEEVLHERVVGQDEAVTVVANAIRRSRAGLSDPHRPIGSFLFLGPTGVGKTELARTLA
                     DFLFDDERAMIRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAVRRRPYAVVLLD
                     EVEKANPEVFNVLLQLLDDGRLTDGQGRTVDFTNVILIMTSNLPGDPHDFFRPEFINR
                     LDDIVRFKPLEPGDLHHIVDIQLGLLQERLTGRRIHLDVTESAKSKIATMGYDPLFGA
                     RPLKRLIQREIGDRLAVALLDGTVAEGSKVTVDIDEDQFVLR"
     gene            complement(37223..37609)
                     /locus_tag="PKY13_00180"
     CDS             complement(37223..37609)
                     /locus_tag="PKY13_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT78364.1"
                     /translation="MPEFDQAVYVISVAAELAGVHPQTLRIYERKGLLNPARTGGGSR
                     RYSQADIAHLERIQQLTNEGLNLAGVTRVLELEWRIKQLEAELERTRAEAAASVADTH
                     RQYRRDLVPVPPTTVVLYNEGRRRRT"
     gene            complement(37718..38851)
                     /gene="dnaJ"
                     /locus_tag="PKY13_00185"
     CDS             complement(37718..38851)
                     /gene="dnaJ"
                     /locus_tag="PKY13_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT78365.1"
                     /translation="MTMQREWFEKDYYAALGVAASASPKDITTAYRRLARQFHPDANP
                     GDSTAEEKFKEVSAAYDVVGDPERRKEYDQVRAMGPMGSRLSGGGPGGGFGRPGDATF
                     NGADLGDIIGNLFGRGEQGGARAPFSRGQQRGTDLEADLHLAFMDAIRGVTTSVNLIS
                     EATCTECNGTCAARGTSPRVCTECGGRGVVDDNQGFFSLSHPCAGCGGRGSFIDTPCP
                     ACSGSGVMTRPRVVKVRLPEGVKDGQQIRLKGKGGPGRNGAPDGDLYVRVNVEPHPLF
                     GREGDDLLLTVPVSFAEAALGADIRVPTLDGDAVTIRIPAGTPNGRVFRVKARGIATA
                     TGKGDLLVAVDLVVPTGLSDPERTALEAFRDASTQSPRAHLGV"
     gene            complement(38932..39531)
                     /locus_tag="PKY13_00190"
     CDS             complement(38932..39531)
                     /locus_tag="PKY13_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT78366.1"
                     /translation="MTDPTNDEVSADDPSADTGVDSVDSGLVEDIDLDETDLAELLDQ
                     IGSTGSAEELLTSALEDLERVTAERDEYLDLARRVQAEFENYKRRIDGQRIEQVQRAA
                     EALVVEILPVLDACDAAIGHGAQDVGPIQSALLNTLEKRGLAKVDDVDVSFDPLVHEA
                     VLTEAGDGADDEPVVTEIMRSGYAWNGRVVRPAMVKVRG"
     gene            complement(39528..41354)
                     /gene="dnaK"
                     /locus_tag="PKY13_00195"
     CDS             complement(39528..41354)
                     /gene="dnaK"
                     /locus_tag="PKY13_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT78367.1"
                     /translation="MPKAVGIDLGTTNSVVAVLEAGDPVVIPNSEGARTTPSVVAFTK
                     DGEVLVGEVAKRQAITNPDRTIRSVKRHMGTNWSVDIDGKAYTSQEISARTLMKLKRD
                     AEAYLGDTVTQAVITVPAYFDDAQRTATKEAGQIAGLEVLRIINEPTAAALAYGMEKE
                     GADQTILVFDLGGGTFDVSALEIGDGVFEVKSTHGDTELGGDDWDQRVIDWLVDSFKN
                     SNGVDLSKDNMALQRLKEAAEKAKIELSQVQQTQINLPFITATDAGPLHLDESLTRAK
                     FQEITADLLDRCRVPFEQALKDAGLTMGQLDHVIMVGGSTRMPAVQELVHSMTGREPH
                     KGVNPDEVVAMGAAVQAGVLKGDVKDILLLDVTPLSLGIETKGGVMTKLIERNTTIPT
                     RRTEVFTTAEDMQPSVEIHVLQGEREMSSYNKTLGKFQLVDLPPAPRGVPQIEVTFDI
                     DANGIVNVAAKDRATSKEQSITITGQSSLSKDQISQMVKDAEAHAEEDRKRKEDAEEL
                     NKADTLVYQTEKLLREQGEKIEGDDRVQVEQALGTLKEAIAEGEIEKIKIATEGLMIA
                     SQKFAQQLYEQAANDGGYTQDSPSAGGAEDDVVDAEIIEDEK"
     gene            41674..42810
                     /locus_tag="PKY13_00200"
     CDS             41674..42810
                     /locus_tag="PKY13_00200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOT78368.1"
                     /translation="MVAVLLAAADLAAQWNIDGNRRIDYRIYVRAVSSIDGAGLYDYG
                     TGILRFTYPPVAAVLVWPFTWLDEAVGSRVWLTLSVVAFVVASALAIHNAGGTRPVPP
                     TVALVGGAAALWLVPAISTLRLGQVNAFVALLLCVDSVAIARRSRWSGLGIGLAAAIK
                     VTPLAVLPVLWVGLRRHDHVVARRAATTFVVVTGATAAVAPGATWDFVQRLGGRAVTR
                     AATTSVDLRVLLRSVIDARAVADAVWLVATVVLVAAALRTAARLGRSDRPDGVGLVTV
                     GMCLSTVVSPFSSVHHLTFATIALVLWAARSTSSWQRAVAVAGLLALLDPTAGEGAPG
                     RWAMVAFCIATVVALPSIVAKRPDPDFVETDRIPQDVSLLQGGH"
     gene            42807..43574
                     /gene="gpmA"
                     /locus_tag="PKY13_00205"
     CDS             42807..43574
                     /gene="gpmA"
                     /locus_tag="PKY13_00205"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOT78369.1"
                     /translation="MTMSDLILLRHGESEWNRLNLFTGWYDCDLTDTGRSEAAAAGST
                     IADAGFSPTVLHTSLQKRAIRTAQLALDEINLLWLPVRRSWRLNERHYGDLTGKNKAE
                     TAAQYGEDQVKIWRRSYDVPPPPIAADNAKNPNGDAQYASLPPDVIPGTECLQDVVTR
                     MLPYWYDAITTDLAAGHTVLVVAHGNSLRALVKHLDGVSDDAITELNIPTGIPLHYEI
                     GADFRPVTAKPTADRYLGDAEAIAAKAAAVAAQATAK"
     gene            43634..44320
                     /locus_tag="PKY13_00210"
     CDS             43634..44320
                     /locus_tag="PKY13_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT78370.1"
                     /translation="MVTLGATAQGLLGFGLNLVAAPVVGLVAPELLPATMVVVGVPLS
                     TMIAVRDRESVDWAGVRWTTLGRIPGTIVGAVVVTLVSVRALGGIVGAAVIVACVVAT
                     RANEHQIGRTNSLAAGGASGFMDTVAATGGPPLALLYQHRPAPEVRSTLATSFLVGTA
                     MSLVGLLAGGNVAGWQLLVALALIPFLAIGLALSRPLAHRLAGRSLRTVILYFAAAAG
                     AAALIRALAG"
     gene            complement(44342..44779)
                     /locus_tag="PKY13_00215"
     CDS             complement(44342..44779)
                     /locus_tag="PKY13_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78371.1"
                     /translation="MTTDTTTPPTESGGSNLGTTAALVGILGGAGAMHFVNPQFFDQI
                     VPKWMPGSVRTVTYVSGVVELVAAALVLNPRTRRLGALVSLLTFVGVWPANIQAALDG
                     GMKGLDPPMDSAAVAWIRVPFQLPLIFLAYQLAQSVTSRESAV"
     gene            44861..45643
                     /locus_tag="PKY13_00220"
     CDS             44861..45643
                     /locus_tag="PKY13_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78372.1"
                     /translation="MRARVQFPEEAVTDTQTWSTTVAAAAVGATKVYGSGDTQVVALD
                     HVSAEFNKGQFTAIMGPSGSGKSTLMHCLAGLDLLTDGSVLIGGDDLSKLKERDLTLL
                     RRDKIGFIFQAFNLIPTLSALENITLPMDLGGIKPDQGWLDTVIDTVGLRDRLSHRPS
                     ELSGGQQQRVAVARALASRPQIIFADEPTGNLDSRTGTEILTFMRKAVDELRQTIVMV
                     THDPNAAGYADRVLFLADGKLVDEMYEPTAAKVLDKMKALGD"
     gene            45648..48269
                     /locus_tag="PKY13_00225"
     CDS             45648..48269
                     /locus_tag="PKY13_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT78373.1"
                     /translation="MLRVALKSVLARKRRLLTTGFAIVLSVAFISGTLVITALMNSTL
                     DSLIGSSYQGIDAIVRSANAQESNFGPPIRPTVPDTTLDLARATKGVRVADGVVQGLP
                     TIIDKNGDRIQDLAGPPTIAFNWIDDPALQGGKLAPGGRAPTAPNEVVLDVRTAEEFG
                     FEVGDQVQAGFPSGLETLDIVGIGGIPVDGQKELVAGPRVLLMQLDRLHELLKKDGFD
                     YIAVSAAEGVSQAEIATNLSKILPPDQEVLTGEQFIAESEATISKLISLFTQPILAFG
                     FVAVFVGAFVIYNTFSIVVAQRTRELALLRAVGAGRRQILGSVMVEAVVVGLVGSAVG
                     ILLGWGLSYGLKQALSGSFSLPAGLPPLTLQAVITGLIIGIGSTMISAFIPGLRATFI
                     PPVAALGETAIDRTALKTSRKVIGALMLLAGIGLIGLVSAGTLDWGLAGRGIGAGLLF
                     LAITTLGPVFAGPMARTLGAPLPRVRGVSGRIGKENAGRNPKRTAITSVALSIGVCLV
                     VIVAIFAASIRSTVKSQLSNQLADVDLVVDSGTGFAGLGPEPRQFLEDQPEVDLINPI
                     RFNQATLLDSKGAKEEQATRGETDNGVPVGESDFVIGVDRTAIFQIAAIEDLTPKITE
                     LPDDEVMVLAKTAEDNGWKPGDRIRVWFPKSGEQTWKIAATFGSRIGNGAEYITNNET
                     QSKNALPEFDVDSTIWVKLNDGVDPKTALAEFKPELKKLAPTAGVNTIGDYVGERLGI
                     LDSVVNLIYVLLGLSIVIALVGVGNTISLSIHERTRELGLLRAVGMTRRQLAESVVWE
                     SVIIALAGTVIGLAIGVVLGTIFVDGIDQQGVEPVVSTVTVAVIGVLGATAGVFLAIR
                     PALRATRVNVLAAISSV"
     gene            48282..48869
                     /locus_tag="PKY13_00230"
     CDS             48282..48869
                     /locus_tag="PKY13_00230"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT78374.1"
                     /translation="MTAEQQRRDDASGQLEESDDDRIRPSPVVARLAGAIVDFTIVVV
                     LVLPIQLLWTHYESFEPDATKVPTGPANWLSLAIIVGYPVISIARYGQTIGKRATSLR
                     VVRADGRPAGWLASIVRFAVSFAPFLTGPIVRQAPAGAGRTALEVLQVILMAAVYLPI
                     PFDDQRRGIHDRVARTRVVTELPPLLRAPISAGRG"
     gene            complement(49151..49531)
                     /locus_tag="PKY13_00235"
     CDS             complement(49151..49531)
                     /locus_tag="PKY13_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT78375.1"
                     /translation="MTDAAHMKSAVEAYLARHSAGDVDGIVELFAADATAWDPVDADP
                     HVGAEAVRAFFTGTHEMVDQLTLTLTGPIRCAGNFAAFPMTVQTKMGDMTLEIDIIDV
                     MTFDADGKFSEMRAYWNMDDARYS"
     gene            49603..50637
                     /locus_tag="PKY13_00240"
     CDS             49603..50637
                     /locus_tag="PKY13_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78376.1"
                     /translation="MSDTTRITELVTGLGMLGGDHPLTMLPVAPGELRNVDAATWDGL
                     VESWHDPALRPLVDAAFENGRFFFEASDALRRRRPRTVEWTGPQRSPGDEVAPVDLRV
                     DHVYLVSCKYLSKITMNASPAHVFERLLTGGHGRRSNVDWYRHVAAAEFDRLWSASAA
                     WLTEHHDGVAVPDDPATLSKPDRKVVSELLRDGGKSWPQELLDPYRELCAAVSVRSAL
                     AWQESISTAPSGAAEAMLWRLLRIGSAPYFVLGVGGAATAPLRLRVASPWDWRQHFTF
                     RRLEITPMSGGQPMVGWRAEFTERDGAAREVHGHVEIRWSHGRFSGPPEAKVYLDTPF
                     GDVPGYWSIA"
     gene            complement(50619..51455)
                     /locus_tag="PKY13_00245"
     CDS             complement(50619..51455)
                     /locus_tag="PKY13_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOT78377.1"
                     /translation="MPEGDTIARSATRLRAAIAGAELHRVEAPRWTGRLPTVGETVLD
                     VRSVGKHLEVWFSGGLVLRTHMRMTGSWHIYRPGERWRRPRRELRVLLVTPEWEAVCF
                     SAPDVEFVTPPPPSAIGQSRPLPATAHLGPDLCLPDADLEACVPRFALVEPTTTIAEA
                     LLDQRICCGVGNVFKSEICWAARLDPFTPVDAVPVSLRRELVQIAAQQLRANLDTARR
                     TTVPGGVAVYGHAGERCRRCGASIRMRRHGVHARSTYWCPGCQVPPSPPDTVEVGQAI
                     DQ"
     gene            51467..51862
                     /locus_tag="PKY13_00250"
     CDS             51467..51862
                     /locus_tag="PKY13_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HOT78378.1"
                     /translation="MPLDPSDPSAEVLEFLSERHLATLSTHRPDGTIHVAPVGFTYDP
                     DRRLARIITRSGSRKVANIDAHPGSRVVLCQVDGGRWLSLDGPAEVTAASDRVAEAVE
                     RYAHRYRQPGERDDRVAIEISVDRMMGRC"
     gene            complement(51882..52928)
                     /locus_tag="PKY13_00255"
     CDS             complement(51882..52928)
                     /locus_tag="PKY13_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78379.1"
                     /translation="MRSNSRPATVVLAALAAVAAVSLLASCSSDDAISPSETTTGIAP
                     ETTTTTTIDPGPTSVPEPGAELPGLWLATSGGIVDETGEVWAKPRPGETLRSPLSDGV
                     GGVVYLRCTGDTLPCVVEDQQLKGVSPLALGEADNLMAIGTYQDHRVLLTGWTDTTVV
                     PSFEEDRSGLVARLIDMDTQQVTPLDGWYGWESGPFAADVENGRFAVCFGEGESCTLS
                     TTEDPSDLPPVPDVDAATVLSLALDATGARLTWIESVPMSGEVKVGTLDLPGGAATTV
                     DIQPANAPASDDAVTDGAWVAVRVGRQVALSQLATTGPAALTGVTGTRTVPDGVTEMA
                     LREPAGGGGGSSPL"
     gene            complement(53015..57637)
                     /locus_tag="PKY13_00260"
     CDS             complement(53015..57637)
                     /locus_tag="PKY13_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT78380.1"
                     /translation="MATPGLDEAGFSEPTAAWFRTTFGEATRAQAEAWPAIASGAHVL
                     LCAPTGSGKTLAAFLSAIDALATAPIRLDDKGNVVESTTVLYISPLRALAVDVEKNLR
                     APIRGSMLAAQRLATDPSSGDAPPVPFREPTVGVRSGDTSAQDRRRLVAHPPDILITT
                     PESLYLMLTSAARETLRSVTTVIIDEIHAVAATKRGAHLAVSLERLDVVADRSPQRIG
                     LSATQRPLSEIAHFLGGNVVDPRSGDVVGPRPVTIVDAGVRKPLDIQVIVPVDDMGAL
                     GEIIDESGGDLPSGPTSAGPVRRSIWPAMHPRLLELILEHRSTIIFANARRLAERLSS
                     RLNELADEQPSRGAENVGSPPAGGELVRAHHGSLSREQRLVIEDDLKSGRLRGLVATS
                     SLELGIDMGAVDLVIQVESPGSVASGLQRVGRAGHQVGAESRGRFFPKHRADLVETAV
                     VVQRMDAGEIETTRFIRNPLDVLAQHIVAMSALDEWTLPELRAAVRASAPFGELSADL
                     LTATLDLLDGRYPSDEFAELRPRIVWDRTADVIRGRAGAQRLAVTNAGTIPDRGLFGV
                     FLPDGARVGELDEEMVYESRVGETFLLGATTWRIEDITFERVIVTPAPGLPGKMPFWH
                     GDGPGRPIELGRAIGEFTRTIRSVPPAEALVTLGERHHLDERAATNLLAFLEEQAEAT
                     GVVPDDRTIVVERFRDEIGDWRICVLTPFGARVHAPWGMAIRGRLEAAGATDVEIMWS
                     DDGIIFRLPESEDALDIDDLLIDPDDVTELVVSMLPNSAMFAARFREAAARALLLPRR
                     RVDQRTPLWQQRQRAGNLLTVAARYPTFPILLETTRECVNDVFDLPALIDLMTRLRDR
                     RVRVVSVDTPVASPFAASLLYEWIAVYMYEGDAPLAERRAAALGLDRNLLRELLGSDE
                     LRSLLDGDVIDQIESELQHLAEGRRARSAEELHDLLRVLGPLPTDELAVRSEVAVSVV
                     DEWVSTLTDQRRAIEVTVAGRREIADAADAGRLRDALGVALPVGLPAAYTESNDAPLR
                     SLVERYSRTHGPFTAAAVAHRLGVPVETAQLALASLEADERVLRGEFRPGGSGTEFCD
                     PEVLRMIRRRTLAALRRDVEAVDPEVLGRFLPAWQRVGSTAKGPEALADVVSQLAGAP
                     LVASSLDVEVLAPRLGDYRPADLDAMCTAGELVWVGAGGIGTKDGRVRLVFRSDTALL
                     SAALSSIDAEAAPGDPHHVAIRERLRDRGASFWTDLLAAVAAADLPYDDATVLDALWD
                     LVWAGEVTNDSFAPLRAKVVSTSKRSSPRRTRSRGLRSMSAAPPAGAGRWSLVADLVP
                     VPPTSTEVSHAQTLQLLDRYGVLTREMALAEGIVAGFAGVYPVLKAMEEQGQIRRGYF
                     VAGLGAAQFATHAAVDRLRQFRSVSDGTGSDAAVGSAFGAASEPTGPPFVLAATDPAQ
                     PYGAALPWPDNAGRPTRSAGARVVLADGHPVAWLDRSGRNLVTFPAAGNDPEWVAAVA
                     GLVRSGRVRRLEITTVDGAPVSESSWADPLREAGFLDGYRGLILRS"
     gene            57716..58402
                     /locus_tag="PKY13_00265"
     CDS             57716..58402
                     /locus_tag="PKY13_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT78381.1"
                     /translation="MAVPTTSKSHKSGEFHPAFEEYCEAIFELQEDAVNVIQARIADR
                     LEISRPAVSEMIRRMAAEGLITVDETIKLTPAGQSLAEAVVRRHRLAERLLTDVLGLS
                     WSTAHAEAGKWEHVISAEVEAALARVLGEPTTCPHGNPIPGSDYEAPDTVALSRLPRG
                     AEFTVSRIPEELEFADGMLDFLEDNLLRPGFEGVVDELVPSGERIVTIDGQTVTIGQF
                     ASERILVSVA"
     gene            58399..>59807
                     /locus_tag="PKY13_00270"
     CDS             58399..>59807
                     /locus_tag="PKY13_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOT78382.1"
                     /translation="MSAHEDGTPVVVTTGAGALRGRWQDGVARFAGVPYAAPPVGDLR
                     FRAPAPVEPWTGERSAEAFGPICPQNPSMMDALFGGDSERWDEDCLYLNVWTPDPEAA
                     ATGPLPVMVWIHGGGFEMGSGSSPLYDGTHFASSGVVLVSLNYRLGAFGFLELGHLDA
                     DLSGSGNAGLLDQIAALEWVQSNIANFGGDAANVTVFGQSAGSMSISLLMASPRATGL
                     FRRAITQSGAANAARAVESAQGDTHDFLAAGGWSSAADVRAAPTADLLAAHASLSGAR
                     VADPEAFIRRAGTPLGFLAFRPVADGVVVPSDPLAAIAAGSAAGVTLLAGSTSQEWRL
                     FALMTPSPADEDALRDRFALLVDDPDAALAAYRDEYPGASLADLDGALLTDMVFRIPT
                     VRLVDAQSAHAPTFQYLWDWKSDAFGGMIGAAHAIDIPFVFDLLEDHRLHVFVGADAP
                     RSLARATNEAWIAFASTGVP"
BASE COUNT         9239 a        21200 c        20156 g         9012 t
ORIGIN      
        1 ccgaacagga ccacgctcat cgcccacgcg ttgaggatgt cgttgtggat cgagaagaac
       61 cggtcgccag ctcggcggcg acggtcgcgc tcgatgcgga cagcagaacg gacactgccg
      121 aacaccgtcc ggggcaggaa ggagtacagg ctctgaccga gacgggagct ggccggatcc
      181 tccggcgtgg acacgtgcac gtggtgaccg cggttgtgct cgacgaagaa gtggccgtac
      241 cccgtctggg cgagggacac cttcgccaac cactgctcga ccttcgggtg cttgtggccg
      301 agttcgtgcg ccgtgttgat cgccactccg ccgacgctgc ccaccgtggc cgcgagcccc
      361 aacgcgtcga tcgtcgagag atcgccccag gcccagtagg cgcaggcgac gacgagggac
      421 aggaactgca acgggaggta ggcgtaggtg caccaccgat agaagtggtc gtcgttgagc
      481 tgcgggacga gctcctcggg tgggttctcg tgatcacggc cgagggtgac atcgaggatc
      541 ggcatcagca cgtagagcac gaccgggccc agccaccaac cccacccggc acccgtggcg
      601 gccaccaggc cggctgccaa cagcggcagc accggcacga tgagaccgag ccagtagtag
      661 tgccgcttgg cgtcggtcca cgactcggat cgacccacct cgacacggga cagctcttgg
      721 acatacggcg ttgtcatgtc gtcatgatgt acacgcacac ggcatttgtc aagcaatcgg
      781 ttcgccgtcc tcccaccgct ccgtcacacc gtgcgcgatc caaccccgtc gggaccgagc
      841 gcgtcgctgc tacgatgcgg cgggccgaaa cctcatgacc acccttcacg gcaggtcggg
      901 cgccggctcc gagtgccccg aatccagggc cacgcgggtg tagttcaatg gtagaacggc
      961 agcttcccaa gctgcatacg ggagttcgat tctcctcacc cgctcaccgc aaccaatgag
     1021 ggattcgccg atcaggcgcc ccctcgcttc cggcgacttg tcagggtctt gtcacgacgg
     1081 atcgggatcg ggccctgacg gggcttgtga cgggcggtca cggccgtgag gtcgagccgc
     1141 tcgttgagct ccttcgctcg gctctcggcg gactctgatg tgggccaggg tgcaggatcc
     1201 tacggcgacg gcaaggttgc ttcatcgaag ccggcctacc cacccttacg acgtacgcgg
     1261 gacttggatt gcataggtgt gattccaagt ctcacgcgcg aaacttggaa ttgatcgcgt
     1321 acaatccaag tcatgtcgct agcaacctca cccgtcggat cgctcgtacc catctccggc
     1381 tacgacgcta gattcggcga ggactaccac gcagacgcgt tcgtgccgac cgacctgccg
     1441 gattgcgttg atctgcccgg cgcagtgtgg atggcgatca gcgatgccaa cgcagagctc
     1501 ggccggctgg acgcagccgc aagtctcgtt ccgaatcctc agttggtgac acgcatcgcg
     1561 acgcgccgtg aggcagtggg cacgtcggcg ctggagggta cctacgctga actgacggag
     1621 ctctacgccg ccgaggtgct cccgttcgac gagcaggaca cagaggtggc tccaaatgtg
     1681 cgcgaggtga tgaactacgc acgcgcggct gacgccgcgt acgggtggat cggcgaacgt
     1741 ccgatcacgc tccaaatgct tgcgtcgttg caggccgaga tagtccgcgg caccgagagc
     1801 gacagccagg aagcgggcgc catacgcgag agccaagtgt tcatcggggc caagcatcga
     1861 cgggtcacgg aggcccgttt catcccccca cccccaggcg atcagctccg cgccatgtgc
     1921 gaacgatggg tggaatggct ttccgcacca gcgccgctcg cgcaaatcca gctcatccct
     1981 cggattgcaa tggcgcacta ccaattcgaa acgctccacc cgttcatcga cggcaacggc
     2041 agagtcggac gcctggtcgc gatcctccag ctgatgagcg aacacgcgtt gcgttcgcct
     2101 gtcctctcgg tttcgccgtg gctcaaggat cgggcggacg aataccgcga tcacctcttg
     2161 gccgtgagca tgacgggcga ctgggcgcca tgggtcgagt tcttcgccaa cgccgtcgct
     2221 gtcgaagccc gatcaggcca cgaacggatc atgcgcctac ttgagcttcg tgagcaactt
     2281 ggagaaacgg tgcgctctgc gctcccgcga gcacgcctcg ccttggagat cactgacgac
     2341 ctcatcgcct ttccaattct ctccgtttcg gtcgctcagc gccgatacgg tcgttctaac
     2401 caggcgaacc gcgacgcaat tggctcactc gtagaactcg gtgtgctcga gccctacggc
     2461 gaagccaggt acgatcgctt gttctggaat cgccgggtat tccaaatcat cgagggctga
     2521 tgtcgagggg ctggctaggc gccgcccccg aggagtgggc ctacgaaccc ggcggttcga
     2581 gcacgccgag aagacgttcg gtgaaggtac acgagacatc aagcgactcg tatgtgccca
     2641 tcgcgaactc cgggaccgga cagacagtca ctgatccgtc tttcgacacc gagccgatga
     2701 ccagagcccg gcggtgcggt gtcgcacaag tgtgttgcac agggcgttcc ggcacgaccc
     2761 ggcaccctcc gaccgccagt actagcgggg ttctcggcac tcgccgtcac tcgacgacaa
     2821 ccgccgacac ccgctctcga gacttcccaa gctgcatacg ggagttcgat tctcctcacc
     2881 cgctccgcac acaacccccg ccatggcggg ggtttcgtcg ttctcggagc cgacatcacc
     2941 ttcgtgggca cacacgaaga acgccctctc catcccgggg ggtgtcgagg gcgtcttcga
     3001 gaaactgcgg gtttccaaga ggggcgccaa cgtgggggca ttgacgacct cgacgactcc
     3061 tgcccgtcat cagcccggtc caaacacctc ggccctcggc gacgggcgcc gaccttcacg
     3121 acactgcccc aatcgggtac cacgaacggg ggacgtgaca tcgcactcgg tgacgtatcg
     3181 tcggtgacac gtccgctccc cgcccggcac cgaaggcagc gcccacatgg ccaacgacga
     3241 tccctccaac gcatccgaca tcgtccacct cgcgggcgac tacgacatcg cgcgccagga
     3301 cgagctgcgg agcgagctgc tctcgtcgga cgccgatgtg gtcgtcgccg acctgcgcga
     3361 tgtgtcgttc ctcgactcgt ccgcgatcca cgcgttcgtc gacgcccgaa accagctcac
     3421 cgcccgcggg cgcgacatcc aactcatcaa cctcaccggc atgccccgcc gggtgttcga
     3481 catcacgggc ctcaccccgt acttcaccat cgccgagtaa cggccgtcgc aacagaactc
     3541 aaccgccggc gggattcgcg agatgatcgc cgccccaata gccggccatg agctcgagcg
     3601 accaggaggg ctccaccacc ggacctcggg gcaggaagcc cgacagcacc ggcttgagat
     3661 cgagcaccgg cgtaccgtcg acagcgtcga ggccctgcac ccgcaccgca agaccttcga
     3721 tcccgaccag tcgggcgacc gacaccccga gccggttggg ccgcactcgt ccccgctgag
     3781 cgaagatacc gacctcgggc cagtcggcac ggccgcgggg cctgcgagcc gacgccacca
     3841 catctgcttc gtcgaccagg tggaagtggt agacgacctc gacgtggctg aagtcggcga
     3901 gcccgacgag cgccgacgct tcgaaccgct cgggatccaa ccggatctcg caggtctccg
     3961 catcccagcc gtcatcctcc ggaaccaccc gaccgccgac gaccgtgccg atcggtgtca
     4021 gttcgattcc catgtcatcc tccgtttcgg tgaccgaggt ggtcgagtac gcccgcagcg
     4081 gcggccaccg ccgtgctgaa cgtggcctgc aagagatagc cgccggtcgg cgcttcccag
     4141 tcgagcatct cgccgacgac gaagactccc ggcagccgac gcaacatgaa ccgctcatcg
     4201 acctcgtcga ggcacacgcc gccggctgtg gagatcgagc gttcgaccgc ctcggcgccg
     4261 acgagacgga gcggggtcgc cttcaccaac gccgcgagat cgacggggtc ggagggcagc
     4321 tcgttggccg tcgcctctcg caacagtccg atgcccaccg gtgtaatacc ggccttgcga
     4381 aacgccgtcg tcgccgagtc cttggggcgc cgtcgcgcca gacgctccgc cacctgctcc
     4441 gccgacaggt cgggccgcag gtcgaggtga accacgacct cgccctcgag ctcgatcagg
     4501 tcgcggatcg tcgaacccag cgcgtagaac acaccgccct cgaccccggt ctcggtcacg
     4561 acgccttcgc cccgcaccga cctcccaccg gccgacagct ccacgttctt gagcggcgca
     4621 ccggcgaagc gtcgacggaa gacctcggtc cacgccgaga cgaacccgca gttcgcaggg
     4681 cggaagggcg cgagaccgac accagcagcc gacagcgccg acgtccacgc gccgtcggag
     4741 ccgaccttcg gccacgacgc tccgcccatc gcgagcacaa cggcatcggc gacgaccacg
     4801 gactcgacgc catcctgatc cgccaccgat acctcgagcc ggccatcgtg ccgtagctcc
     4861 acgccgagcc agcgatgacg ggtccggatc acaacgccga gcgacgccag ccgtgccagc
     4921 cacgctcgca gcaacggtgt ggcccggaag ctctcgggga acacccgacc actcgacccc
     4981 acgaacgtcg gctcgcccaa cgactccgcc cacgcccgta gcgcggccgc gtcgaatcgg
     5041 gcgaccgccg gcccaacccg cgacgacgcc gagccgtagc gctcgaccag ctgctccgtg
     5101 ggctcggaat gggtgaggtt gaggccgccc cggcccgcca gcaggaactt ccgcccgacc
     5161 gacggcatct gctcgaacac cgtcaccgac gagcccgagt gggccagctg ctcggccgcc
     5221 atcagaccac ccggcccgcc gccgacgacg atgacgttcg gagcggttgg catgccgccg
     5281 aggttagggg cccgccagca cgaccctcgt gccgacgacc gcaccaatgt cagagcgggg
     5341 acggttcgat gtcgtcagca ggccagaggt cgaggggctc ggcggcgagg tcgacggccg
     5401 ggtccatcaa gcgcgtgcgg ggaaggccgg cgatcgccac ccaggcgtcg acgcgaacct
     5461 cagggcgcgc ggcaccgctc cgttcggtct cgtccgcgag atggtgcgcg tactgacgga
     5521 tcagatcggg gcgcaccgac atcgaccgca cctgatgtgg ggccagctcg tcgctcggcc
     5581 acaccgtgcg ggtcaggccc gtggccgggt cggtcacccg gaacgtggcg cttccttcct
     5641 tctcggtgag gagcacccgc cacgaccagc gatagccctc ctcggtccac cgcacgtcgc
     5701 cggggtacgc gagatgacgc aacggcacca ccagctgcac caccacccag accacgacgg
     5761 cggccgtacc gagccggacc cgccacgccg acgacggcgt cacggccgcg gcggcaaccg
     5821 gtgtcaccgg cggcaccgac acgagcccga accgacggcc caccgcccca cgaacccggt
     5881 cgggccaatc cggctcgaag aagatcgggg tcagcacgat catcacgaac ggaaaggccc
     5941 cgatgccggg gaacagccat cccgtgatca ggtggaacac cacgaccgcc gcgtacgcgt
     6001 aggggcgggt gcggcgacgc agcaggaacg caacgacccc gaggtcgaac aacgctccgc
     6061 cccacgagag cgcaacggcc acccatcgat cggcgaacca cggaccgacc aacggaaggt
     6121 cggtgcggcc cagcaaccac gtgtgcagcg gttcgccgcg cagcaaccag tcgacgtcga
     6181 ccttgccgag cccggcgaac acgtacaccg ccccgagctg gaagcgcagg agccacaccg
     6241 cccacgccgg caccgtcgac cgcactgccg aaccacgtcc ggggaggggc agcaccacgc
     6301 agaggacccc gatgagggtc acgagctcgt agtggttgag gtaggtcgta acgtcgatcg
     6361 cctccgcgta cgcgaatccc agcgtgaagg cgagggccgc ccaccggggc cgccacccga
     6421 gcgcgagcgc cagaccggcg agcaccatga cggcgtagag cgcgtacatg ccggcggccg
     6481 gcaggggccg gacccacgag agccccggat aggtgaagtg gtacgagggc gcgaggtaca
     6541 ggcgctccac ccagcccctc gagacgaacc gggccgcgac caccgcagcg gacaatccgg
     6601 cagcaacccg gaaggccgcg agcgacgacg ccgggaccgg acggaggccc cgctcgacca
     6661 gggccgcggc aagcccgcgc cgagacctca ccaccggcgg agcggcgagg tcaggaatcg
     6721 ccgtcgttct cactgaagct gaccgtgacg tcgagcgcgc tcacgacctc ggtcgcgatc
     6781 gtgacacgga gctcctcgac cgccgtcgcg accgccttgg cacgatcggc ggtcggcggg
     6841 gtctccgacg gctcggtcgc gtcgtcggcc aggatccgcg cgagggcgtc aacctcatcg
     6901 gagatggcct tcgacagctc cggcttcaac aacgcgtcca gctccgtgta cgagtcgccg
     6961 atgccccgca cctgcgcggc gaactggaac atctctccga ccggcaggtc cgagtcagcc
     7021 cccggagcca gttcgctctc cgcggtcagc agcttcgacg tcgactccag ttggctcaac
     7081 tgcatgttga ccagcgagtc gatcatgtcg gtcggggaca gcgcatcgtc gccgacaccg
     7141 gtcgcagtgt ccgaataggg cgccgccccg tcgagccccg tcgtccaccc atcgtcgacc
     7201 gtcgccgtct cgtcggcgat gacgctcatg acggccgaca ggtacgcaca ccgtgccgga
     7261 acggcggaga acgctcccgc gtcggccgtc aggagatgcc aggcagcgcc gtagccacga
     7321 aggctcgatg aggttcgatt gcggaccgtc tcgggatcgg tggggggcga cccggcgagc
     7381 gtcgcatcga ccttcgcgac gtcgaccttg taggacacca ccgaggagga gcgccgctgc
     7441 atcgccgggc cgacgtcgaa cgagtccgtg gcgcgccagg caccccacgc ggtggcgacg
     7501 gccgagcgcg ccacctcgag ccgcgtgctg tcgggagact cacacaactc gccgatcgcc
     7561 gtgctcgctg ccgccgcctc cgtcgcggcg gacgcattcg ccgggaggat cacgtcctgc
     7621 acgagcgccg ccacgacctc ggcccgatct gccgtggccg gcgcagcctt cgtcggttcg
     7681 tcatcggacg agcagccggc gaccaaggtg gtcacgaaca gcacggccgc cgccgccgat
     7741 acacgcgaac gcgcccggga cgggcgctgc cggttcatcg ggactccagg aagtcgatca
     7801 gagcgtcgcg atccttcttc ggcaggtcga gtactgcgtc gcgggaggct tccccctcgc
     7861 cgccatgcca caggatcgtc tcgagcaggt tgcgtgcccg gccgtcatgg aggaaccggg
     7921 tgtggccgtt gatcgtctcc accagaccga tcccccagag cggcggcgtc cgccactccg
     7981 aaccggacgc ctcgaaatcg ggccggccgt cggccaggtc cgggcccatg tcgtgcagca
     8041 ggaggtcggt gaacgggtgg atcgtctggt tggccaacgc gtcgatgtcc gacgctccgg
     8101 tcgtcagctt cggcgtgtgg cagctggcgc atccgctgtc ggcgaagacc tgggcgccgc
     8161 gccgcacgtt cttgtcgtcg gcgtcacggg ccgcgggcac cgacaggacc cgcgtgtaga
     8221 agacgacggc gtcgaatgtg tcgtcgtcga tctcgggcga tccgccgctg ggcgcagcgg
     8281 cacacgccgt ctgtgcctcc ggacagtcgg cgtcggggaa caccgggctg gtgatgccga
     8341 cgtcgccgct gaacgcgccg gccgtctgct gccgaaccga cgcgacattg gccttccagc
     8401 cgaaccgccc gattgcggtc gtgcccgcga cctcgtccca caccgtgttg gtgcgaccgg
     8461 agatgccatc cccgtcggcg tcgtcggggt cggcggctcg ttcgatcgtc gcggcgggca
     8521 cggcctcgag gagacccatc ccgggaatct gcggcgcaat gcgaggaccg atcaggatct
     8581 cgcccggttc cccgaacgcg gggtcgccga tctcgtacac cggcttgcgc agggtgtacg
     8641 tcgtgccgtc ggcgaacgct ccctcgatgt cctcgtaggt gatcgcgatg cttccctcgg
     8701 caggaacacc gggaaccgac cggtcctgga gctgaccgcc gtacgtggga tgggccttcg
     8761 gcgcaccgtg cgccgccttg ccggcgatgc tcagccgcag gagcagcccc ggcgtcgccg
     8821 gcccaccctc ggcgggcggc tcggctcgac cgtcgaacgt gtggcacgag gcgcacgcgg
     8881 tggcgttgaa cgtcggaccc aggccgtcgc ggctcgtggt cgacgccggt gcgatcaccc
     8941 agttctgggt gaagaagctg tcgccgacct cgaagtccgc ccggctgctt cccggcaggc
     9001 tcttggccgg aagtccgaac gcgttctggt tgacgacgtt gcgggtcgtg tcaccgccga
     9061 ggtcggagtc gaggagatcg gccagcgcga cctcggccgg gcgcgccgcc cgctcgttcg
     9121 aacacccggc caatcccatc gcgatcgtcg ccgccgcgac gatcgcgatc ggaacggcac
     9181 gcttcatcag ctgacctcga ccgtcactcc gagcgcctta ccagcggcca cgatcgaatc
     9241 cgtctgcgtg ccgagatcct cgatggcgtt gcggatcgcg gtgcggcccg ggccgtcgtc
     9301 gggggcatcc gccgtgaggt tctggtcgaa cggcgccggg atcttcgaga tctcgagtac
     9361 cgactcggag atctgcgccg tgatgtcgga cgacaacgcg gcgtcggcgt cggcaacgag
     9421 cgacgcgaga ccgggccccg aagcgacacc ggcgtacgag ccgagccaca cgttcgcgat
     9481 cccgttggcg ttggcggaca gatccgcagt ggtgttgtcg ctgaagcagg agtgctcgtt
     9541 ctcctggtcg cgctcctcga aggcgacgtt catacgctcg ccggcgagtt cgccgcggga
     9601 cagctccccg atgccggtga tcatgtcgct cagcgtctcg gtcaccggcc tggccacgaa
     9661 ctcggagcgg taattgtcgg cgtcgggtgc ccattggtcg gtgacctgcg tcaggtcgtc
     9721 gatcaagagg tcggtgaccg ccgacaggta cagcttgcga cgctcggcgt tggcagcggt
     9781 cgtgtagtcg gtcgccggtc gggcaccggg cccggtgtca ctctgatcct gaccccacag
     9841 gaggaactcg atggcgtgcc agcccgtcga gaggttggcc tcgccaccct gctcgttggc
     9901 cgccgccagg gcgtccttgg tgatctcggg gaagcccacc gtgtcggcga tgatgcccga
     9961 gtcgggagtg tcgaccgtgg agtcgatgta ctgctcgtcg agcggccatc cgttgatgcg
    10021 cccttcggga ccatcttcct tgttgtcgat cgggccaccg tagaaccgga atgcctcggt
    10081 gagtccgtag ggcgtccggg cggcgatcca tgccgacctg gccgtggtga gcgttgcctc
    10141 ggtggggtcg gcgacgaagg cctcgacggc agcatcgagt gccttcgctc cgtccagcgt
    10201 cgcggtgtag ctggcgaaga cgccctcggc gtagtgggcg acgacctcct cggcaagcgc
    10261 ctcgttgagc ttgcccgacg ccgccgtcgt ggtgggttcg tcatcggtcg ccgagtcgtc
    10321 accactgcat gccgacaagg tgagaccggc gagtgcgatc acgccgatcg tccggatcgt
    10381 ccgactgccg actcttcctc caactcgtgc cactgctgct ccatttcgcc cgaccgatcg
    10441 agcacctcgt ggcgctcgtg ttaggtaagc ctaattccag aaccccgggc gcgcgcaact
    10501 ccttccgccg ggacgcgaaa cggggaggga caggcatact gtccctcccc gcctcgccac
    10561 ctcccccgac cgcagccagg ccccgaacca accgtgccga ccggggagtg tcgcccgatg
    10621 gtcagggtgc gatgttgtac gccaccgtca gcggatcggt cggcttgttg acgtcggagc
    10681 tgctcccgct cgtgtaccag taggcaccga ggccggtctc gatctggaag tcgacgaagc
    10741 tcttcggcca cgcaccccag tacccggcgt tcgccgacgt cttggcgatc tgcggcgccg
    10801 cggtcgcgtg cgccgacggc agttgggccg cgacgttcag gaaggccggc gtgccggtga
    10861 acccggacgt gttcgcggcg ctgaggccag tgaactccgc cagcgtcacg gtccgctccg
    10921 gcagctggat ccggatcgtc ggatcgtcct ggctcgacgc gaacccggcg atcgaggcgg
    10981 tgagcgtgcc cacaccggac ccgttcacca cgagcttggg gttcttgatc cagaagggca
    11041 cgtaggtgcc gtagaagttg atcgtgaagg tgccggtcca ctggatggtc gaggcgccgg
    11101 tcgcggtgtt cacggtgccg gtgccgccgg tgtagcgaac cttctggccg aggaagaacg
    11161 cgttcgacga ggtgaccacg ttgcccgctc cgtcacggtt cttgttggcg aagctcacgg
    11221 cggtctcgct gccgatgggg acgtaggtgc ccgcggcgtt cttcttcagg accgtcgcgt
    11281 tgccattggt ggcggtgtag gtcgcagcgg tgccgtcgga gatgcccgcc gaccagtagt
    11341 tcacctgccc gggggcctgg ctggcgttgt tggtctgctt gctcacggtc cactcgaacg
    11401 acgcgttggt cagggtcttg atgctcgacg gcgtggtcgt tgtgggcgct gcagtcgtgg
    11461 tgggcccggg cgtcgtggtc gtcccactgc cggcggaggg cgggggctcg gggcaggcgg
    11521 tcgccaccag ggcggtcgcc ccgaggatga cgccgagcag tgcggtggac cggcgacggt
    11581 gccgggtcgg acgggtggtg gtcatcgcat ctcctgggtt gttattaggt gtacctaact
    11641 cgtttggtag tcttgcctaa cattcggtct gacgcaaccg tctcgttcgc ggtcacaccc
    11701 ccgccgcccg ccgacgccac accacgactc caccggcggc gacgagcggc actccgacgg
    11761 caccgccgac gacccatccg ctcatgcccc cgtcgtcgtc ggactcgaac acgatgaccc
    11821 tgccggacgc ggagttcgtt tcgtcggtgg ccgaacgcct gcgggtcgtc gtcgacgact
    11881 cgaccgcggt cgtggtcgtg gtcgtcgtct cagcagcggt cgtctcggtc gtcatcgtcg
    11941 tcgtcggctc gaccgggctc gtgtcgaccg gcgcggtcga cccgggagac gtcgtggcgg
    12001 gcccactctt cttcgccgtc gtcggggttc ggccggtgct ggatccgccc accaccgacg
    12061 gcgcagtggt cgccgtctgc cccgcgcccg tctgccctgg ccccgtcgac ccgcccgggt
    12121 tcgacgaagc gttcggtggg atcgtcaccg gcgccccggc cccggtactg aagctgatgg
    12181 gagtgaagcg ttcgttggtg gcgctcgcct tgccgtgcgc gccgatcgtg tagatgccgc
    12241 actgcacctg gaggcagtcg acagtgttcg tcatgttggt cgcagggtcg acccattggt
    12301 aggtgctccc cggcaccgtg atcgtcgtgg tccagttgcc acgcgagttg tcggggaacg
    12361 ccgggtccat cgacatggcg aacgcagtgg ttccgtcgtt gacctcgccc tgggtgaagg
    12421 ccacgaaacg gttgagtcct gagccgtcgt cacgcgtgtc ggcaccgcgg ttcacaccgg
    12481 cgtaggagta ggtgatgccg aactgaccgt tcgagttggc gccgttgcga ctgctcggcc
    12541 cccacgtcga ccccggctgc acccaaccga acatgacgta cacgccgccg aacacgttct
    12601 tgtccgacga gtgcggcgga acgaggtagg acgttccgac cacggtgacc gtcgtcgcct
    12661 gagcggcagg caggaccgac tggctgaccg tcagcgtggg cgtctgggca ccggcgggag
    12721 cgacacccag accggccacc gcggcaacgg agagcacggc gcccgcggcc gcggcgagga
    12781 gggttcgagc gttcatgttg cacccttcag gagcaccgaa ccgccgaagc ggtcggagac
    12841 agttgacgtg gacggagatg gggacggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnncc ggtcatgacg gcttcggggc gaccgtcggc agcgacaccg ccgtcgtgga
    13021 tcacgaccac acgatcggcg taggccgcgg cgaggccgag atcgtgcagg acgaccacaa
    13081 caccgtcacc gacaccggcg cgatcacggg cgatgccgag cacctgctcc tggtgggaga
    13141 tgtcgagcga cgccgtgggc tcgtcgagga gcaagagtcc cggctcctgg gcgaggaccc
    13201 gcgccagggc actccgagcc tgttcgccgc ccgacagcga cgggaagtca cgccgtgtca
    13261 acgcgctcac gtcggtgaca cggacggccc agtcgtacac gtcgtcgtcg gcgtcctccc
    13321 ggtcggtgtt gcgccacggt tcgcggccca tccgcacgat ctgctcaacg gtgaacggga
    13381 acgacacgtc actgcgctgg ggcagcacac ctcgacgcat cgcgagctcg atcccggtcc
    13441 acgctgcgac cggtgcgccg ctcaccagta cggtgccggc ggacggctcg cggtcgccgg
    13501 ccagcacgct cagcagagtc gacttgccag cgccgttcgg cccgacgagc gcgacgacct
    13561 cgccggcgcg gacgtcgagg tcgacgccgg cgaggatctc gcggtcaccg aaggacaccc
    13621 cgatgccccg cgccgagagc acgacgtcgc cccgacgcgc cactgcaggg atccgcacga
    13681 tgcgacgcca actcacgccc atcctccggc acggttgcgt tcccgtcgca gcaaccagaa
    13741 gaagctcggc ccgccgagca gtgcagtgag catgccgatc gggagatcgg cgaagtcgac
    13801 ggccgtccgt gcgaacaggt ccgcgaccac caggacgagc gctcccccga gcgcgcatgc
    13861 cgggatcaac cgcgggtgtg acgggccgat cagcatgcgg agcaggtggg cgacgaccag
    13921 cccgacgaag ccgatgatgc cggcgaacgc gaccgccgga ccgacgagca acgccaccat
    13981 gacgatggtg gtgatgcgca gccgctccac gttgaccccg agatgccggg ccgggcgctc
    14041 accgagcgcg aggaggtcga ggcgacgcgc caccgagatc gccccgacga gccccaccac
    14101 gatcaatggc gacaccgcgg ccaccgcctg ccaccggatg ccgttgaggc tgcccagctg
    14161 ccagaacacg atctgttcgc gagcctgcgt gtccccgagg aagacgaaga agccgatcag
    14221 tccggcagcc acggcgttga ccgcgatgcc catgaggacg atggtgacga cctcggtccg
    14281 gccgttcgag cgggacaggc cgtacacgag cagcgtcgtg atcaagctcg ccatgaacgc
    14341 agcgaccggg gtggtgaacg tgccgagcac ggtgaccttc cagacgatca ccgcacaggc
    14401 cccgaccgca gcacccgagg agacaccgac gacggccggc tccgccagcg ggttgccgaa
    14461 cacgccctgc atcagcgcgc ccgccacgcc gagcgcggca ccgacgagta cggccatgat
    14521 cacgcgcgga aagcgcacgt cccagaggac ggcgtcgccg ttggagtgcg ccggcatcgc
    14581 gccccagtcg aggccgatcc ggtggaacac cgatccgagc acctcgccgg gagcgacgtg
    14641 caactggccg gttccggcgg cgacgagcac ggcggcgacg agcgcgagcc ccaacactcc
    14701 gatcacgacc gcggcccgcg accgcgagtg caccgtcgag gcgctgtcgg tgacgacggg
    14761 cacggtggcg gtcgccatca gtcccccgcc ggcgtgccgt agaccgcctc ggccagggcc
    14821 gacagcgtcg ccgggtactg cggcccgaag ctgaggatct gggagtcggc catgtccacc
    14881 acgcactcgt tggtgccggc tctggtctcg gcgacgccgg ggatctcggc caggccgctc
    14941 accccgccga ccgactcgag accacccgtc atcaacagga acatgtcagg agcggccgtg
    15001 acgagtccct cggcgttgat cggtttgtag ccgtcgagtt cgacttgcgt ggcgacgtcg
    15061 acgccaccga gcgcttcgat gaggtcgtcg gcgccggatc cctcgccgaa ccagtagaag
    15121 acgccggcgc tgccccgcag gtagaggaag accatgcgca tgcccttgag cggggaggcg
    15181 tttgccatct ccgacacctg cgtctcggcc gccgagatct cccgttcgac ccgcgacgcc
    15241 accgcgtcgc cgactgcgtc caccccgagg gcgtgcgcga cggcccggat ctgctcgccg
    15301 acggtgtcgc gggcgcggtg gtcgtcgagg atcacgaccg ggataccggc atccttcaac
    15361 tgcatctgca cctcgagcgg cccgatgctg tagtcggtca ggatgacgct cgggtcgagg
    15421 tcgaggatcg cctcggcttg gagctcgtgt ccgttggcgg tgacgacggg gaggtcgacc
    15481 aacgctggca cgccggtcga tacgtcacgt ccgacgagcc gatcgccgag accgagcgcg
    15541 tacaccgtgg tgccgagcgt tccgtacgtg tcgagggcga ggatgcgact ggtttcggag
    15601 atcgtgatcg actgacccgt gacgtcggtg acggtgacgg ggaggttggg cgacggcgcc
    15661 ggttcgaggg gtcgcaccca ctcctgtgac gtcgcggtac gagcgccgac gcaaccagaa
    15721 gcggtcgggt ccgccgcgga ccggtcgacc ggggcgccgg agccgccgca cgcgctcagc
    15781 acgaccgcac tcgcgacggc ggctgcgatc gccaccatcg aggctcgctg ggttcgaagg
    15841 atcgtcggca tcgggttctc tcctgccgca ggtcggatca cggcggtcgg taggcatgcc
    15901 taacgtcgcg tatgttgctc agctacgcaa gccggtgagg acgacgaacc cctggaatgc
    15961 cctcgtcccc ggaactgttg caggcgtcat gaccgacacc gacacgtcgc gcaccaccga
    16021 cccggtgctg caggaggcgc ccctgggatt ccagtggccc acgatcgacc cgttcctctt
    16081 ctgcgcccac caccgcgacg actaccccgc cggcaacgac gcgctcgggc ccgacgctcc
    16141 gctcgtcggc cggtccatcg gccaggactt cgatggcgtc gacggctgga acatgtacca
    16201 cggcgatgtc gttcccgggt tcccgcagca cccgcaccgg ggtttcgaga cggtgacgtt
    16261 cgtacgatct ggcctgatcg accactcgga ctcgctcggc gccgccgccc ggttcggccg
    16321 cggcgacgtg cagtggatga ccgccggccg aggggtcgtg cactccgaga tgttcccgct
    16381 gctcgaccgg gacggaccga accccgccga gctgttccag atctggctca acctgccggc
    16441 cgaggacaag ctcgtcgacc cctatttcac gatgctgtgg aaccacgaca tccctcgcat
    16501 cgccacgagc ggcgacgacg gggcgaccgc cacggtcacc gtcatcgccg gacggctcga
    16561 gggcgtcacc gctcccccgc ctccgcccaa ctcatgggcg gctcgctccg aagccgaaat
    16621 cgggatctgg cacatctcgc tcgatccggg agcgtcgtgg gaactgccgc cgacggcggg
    16681 cgaggacacc gtacgcaccc tgtacctgtt cgaagggtcc gaactcacga tcggcggcca
    16741 cgtcatcggg gcgtccaccg gagccctgct cgcgtccgat gcaccggtcc ggctcaccgc
    16801 gaccggcaca gcggtcgagg cgctcgtcct gcagggccga ccgatcggcc aacccgtcgc
    16861 ccggtacgga ccgttcgtca tgaacaccga agccgagatc gaacaggcct ttgccgacta
    16921 ccgggagacc gggttcggcg gctggccgtg ggacgtcgat gcgcccgtcc acggacggca
    16981 ccagggccga ttcgcccgtc atgccgacgg tcgccgtgag gacatcggtg actgacgcgc
    17041 acctcttcga cacaccgatg gtgatgagtt cggcaatcgc accacgctgc gccgtcgacg
    17101 ccacgatcga ggcggccacc tcccggcgcg tctgacgcgt cgacgtcacg ttccgctcct
    17161 cccgttcgtc ttccaccatg acgccccgac tccggggctc caccgtctgg tagatacgaa
    17221 cgaggagcgc atcatgggaa cacctgcaac ggagaccgac gtcgtcgtga tcggcggcgg
    17281 gctggccggc ctgaccgcag ccgcgttcgc cgcccgggcg ggagccaccg tccgcgtcct
    17341 cgacgcccgg accgatctcg gtggccgggc ccgcaccgcg gtgcacgacg gctaccactt
    17401 caacgagggc ccacacgcgc tctacacggc gtccgacggc gcacgggcgc tccgggagct
    17461 gggcatctcc ccgaagggcg gtaccccgcc actgctccgg gcgaagatct ccctcgacgg
    17521 ccgcctgcgc gtcgctccac cacgacgcgc gctcgcccag ttcgtcggtc tcctccgccg
    17581 cgtcgggacc gaccgcaacg atccgggcct cgttcgcatc agcgcccgcg agtggatcga
    17641 agcgcgcatc gccgaccccg tcggtcgcca cgtcgctgcc ggcgccgtgc gggtcagctc
    17701 gtacagcggt gatctgtcga ccttctcggc ggacgccgcc gcgacccagc tccacgccgc
    17761 gctgcgcggg gtcacctacc tccacggtgg gtgggcgcag ctcgtcgacg cgacgaaggc
    17821 agcggcgaca gcagccggcg cgacggtcga gcacaccagc aaggtcacat cgatcgacac
    17881 cgacggcgaa cgccacctgg tcgccctcgg tgacgggcga cggctgatca cccgcgccgt
    17941 cgtgctcgcc gccggtgggc cgtccacggc cacaagactc gtcggcgacc ggagccccac
    18001 actcacggcc gctgacctcg ccgcgatccc ggtccacgcc gcgtgcctcg acctcggcct
    18061 gcgtcggctc acgacgagct cgacgcgctt cgtgctcggg atcgaccggc cgacctatgc
    18121 cagcatccac acacccgggg cccatctcgc agacgacggt cacgtcgccc acgtcatgta
    18181 ctacgagccg ggcgacgaca tcggggtggc agatctcgag gcgctcgccg acgagctgca
    18241 gcccggatgg cgcgacgagg aggtcgctcg gcagatcggg caccgacgcg tcgtcgcgta
    18301 cgaccgcccc cagccgggca ttgggttgcg aggccggccg gggccgatcg tcgccgactt
    18361 gcccggggtg ttcgtcgccg gcgactgggt cggcccgacc gacctgctgg ggacggcggc
    18421 aatcacgagt ggtaaggccg cggggatcgc cgctgcgacc caccggtcgg ccttctcgac
    18481 ggtcccggcc tcggctcgat gagcgccacc cccgacgtcg cggccgaacg cttcgagcca
    18541 catcgcgacc ggctcttcgg cgtctcgtac cgcatgacgg gttcggtcgc cgatgccgag
    18601 gactgcgtgc aggaggcctt cgtgcgatgg cagcgcaccg acatcggggc ggtcgaccac
    18661 gacgaggcgt ttctcgtgcg gacggtgagt cgactcgcga tcgaccggca gcggcaggcg
    18721 gctcggcggc gggagaccta cgtcggaccg tggctgcccg aacccctcct ggctcccgtc
    18781 acccagctcg ccactgcgtc ggtcgacccc gcggacgagg ccgaactcgc cgactcgctg
    18841 tcgttcgcgt tcgtggtgat gctcgatcgg ctctcggcct ccgagcgggc cgcgttcatc
    18901 ctccacgacg tgttcggggt gccgttcgac gagatcgcga ccacgctcga gcgcgagccg
    18961 gaggcgtgcc gccagttggc cagcagggct cgtcgcaagc tccgcgacac cgacgcgtca
    19021 gcggacgcag ggccgcgccc ggcggtcggg accgatgccg ccaacgcact gggagcgctc
    19081 ctcggcgcgt tgctgaccgg cgacgtcgag gcctgcctgc accacctcgc gcccggcgtc
    19141 gtgctcacca gcgatgccgg cccgcaccgg cgcgccgcac gccggcacgt ggtcggcgcc
    19201 gaccgggtcg tgcggctcgt gaccaacctc ttcgcccgcg acgtcggcct cgtcaccgag
    19261 atccgcccgg tcgcggtcaa cggtgcgatg ggcctgtacg tcgatcacgt cgagggtccg
    19321 ctcgtgttca cgggcgacag cgggcccgac ggtcggatcc accacatctg gatccagatg
    19381 aatcccgaga aggtcagctc gcctcccgac cggtaaggtc gggccactgc cgagctgccg
    19441 gggggcgaca cgatgggaac aggtctgcac acgcgggcga accgacggct ccgttcgatg
    19501 gcgatcgggg tggtcgccgc ggccgtcgcc gccggtgcgg ggtgtgcgcc cgacatcggg
    19561 acgctcacgc cgcaacagca ggtcgaccag ctgatcgcgt tcgtcgaatc ggcgcggggc
    19621 caccagttcg tgactcgccc cgacgtcgag ttcgttcccg acgcggtgtt ccaacagcac
    19681 gtcctcgact cgatcgaggg cgccgaggcg gcgctcgacg tcgacgaggt cgcgttcaag
    19741 gcgctgggtt ggatgccgac caccgacgac ctgttcgaga agtacaagat cgcgttcagc
    19801 ggcaccgtcg tcgggttcta cgacccggcg agcaaggtcc tcgaggttcg cggcgaccag
    19861 ctcacgccgt accgtcgcga ggttgtcgtg cacgagctca cccacgcgct cgacgaccag
    19921 ctcttcgacc tgaacgagtc gatgggcgac ggcgtgctga gcgagcagca gctggcgttc
    19981 ctcgtcggcg tcgagggcga cgccgtccgg acccaacagg cgtacgtggc gacgatgaca
    20041 cccctcgagc agttgcagaa cgtcgcggaa cagctgtcgt tccagatcga tccggcgatc
    20101 ctgtcggtgc cactcgcgct gctcagcctg agccagacgc cgtacctcca aggtccccag
    20161 ttcgtcgccg gactcggcgg caacgccggc atcgacgcga tgttcgaccg gtacccggcg
    20221 acggccgaac aggcctggaa cccggcgaag tacctggcca acgagggagc agtggcggtg
    20281 cccacacccc ccgccggcgg atcgatcgtg aactccggtt cgtggggaca gttcttcatg
    20341 acgctcctcc tcggcgaggg cgtcaacctc gatgccacgc tcaacccggc caccgacggc
    20401 tgggcgggcg actcgttcgt gacctggacc tcagggaccg cgtcgtgcat ccggatcgac
    20461 acccgcagcg acacagcgcc gcagtcggac gcgttgcgcg gcgcactcac gacctgggcg
    20521 tcgagccatg gcgcgatcgt gtcggccgtc gatgcgacca ccgcccgtgt gaccgcctgc
    20581 aactgacctc gccaccgggc gggtgtgaca cgatgcaccc gatggctgag cgaggcaccg
    20641 cagcagaagc agctcccgag ggacggggag agcaggacag cgtcggcgaa gccggcacga
    20701 tgaggggcgg cgtgggtggg tggacctgag cgacggtggc ctcgccaggg gggcgaaccc
    20761 gctcgcttcg cagcggcggc cgacctgttg aaggccctcg caagccctca ccgggtggcg
    20821 atcgtcgatc tgctcgcggc aggaccgctg cgagtccacg agatcgtcga ggcgctcggc
    20881 gtcgaccaac cgctcgtgtc ccagcacctc cgggtgctcc gtgacgccga cctgttgacc
    20941 agtgagcgcc acggcaaaga ggtcgtgtac tcgctcgccg acgagcacat cgcccacatc
    21001 gtcggggacg cgatcaccca cgcggccgaa gtgccgggcg gtgaatcgtc gtagccggga
    21061 ggccgagggc atcagggcta gcgtgaccgg cggcccatgc agatcgtcta cctgagcaac
    21121 cgcccgcagg tgatgcggga aacgtggcag cacgtccggc acttcatgcc gtggatcgac
    21181 cgcgccctga tcgtcgcacc ggcggcgcgg cacggcgagt tcgacgcctg ggtcgacccg
    21241 accgagatca cgctgttctc cgacgaggag gtgagcggcg cgacgagctc gacgctcggt
    21301 gtgctggacc acgtccgacg caacgtgcga ctccgacggg ccctcatcgg ctccggggcg
    21361 accgacgacg tcttcctcct ctccgacgac gactatcgcc cgatgcggcc cgtcgaggag
    21421 tcgttcttca ccgacggcag ccgcgacatc ggctacttca gctacgagct cggcgagtgg
    21481 ccggggtacg agacgccgtt cgacgaggcg cagcacgtga cccacgacgc cctcgcgttc
    21541 ctcggcctcc cgcacctcgc ctacggcgcc cacatgcccc agatcatgcg acgcgacctc
    21601 tggggcgaag cgttcgagct gttcgagacc gtcagtgacg acgacatggt gtgcgaatgg
    21661 gccctgtatt tcaacgtcgc ccaggcacgg catcccgagc gcttcgacgc tccacgcccc
    21721 ttcgaggtga tggggtggcc gcagtacggg gccgagtggc cctggtgggt acgacccccg
    21781 cggtggacgt tcgagaactt ctaccccgac atgtacgagc cggggcacct gttcgacggg
    21841 ctcgcgaccg ggctcgacgc cgacggggtg acgaaaacga acttcgacaa gatcacccgg
    21901 tggaccgcgt tcgcccgggc cgcagaacgc ctcgacttcc ccgccggggt gcacaacccg
    21961 tggacgaagg actcggccct ccgccgcgcc tacttcgcgg cgttgcgccc ggcccgcaag
    22021 acgttgcgct acctcaccga gacccgcggc tccggcacgc gggacgccga ctgatggcgt
    22081 cggcggggtc ggccgacgag gatcgacccg gagtcgtcgg tgtcgtgctg tcgttcgcac
    22141 gccggccggt cgtcgccctg ttggcactgg cggttgcagc gtcggtgctc ggccggttct
    22201 tcgtgcgttc gccgctctgg ctcgacgaag cactgagcgt caacatctcg aagctgccga
    22261 tcggcgacat cgccggagcg ctgaagcacg acggccaccc accgctctac tactacctgc
    22321 tgcacggctg gatggaagtg ttcggcgatg gcgatcgcgc cgttcgggcg ctgtcgggcc
    22381 tcatcacctt gggcaccctg ccgctcgcct tcctcgtcgg acggcggatc gccggtcgcc
    22441 gtctcggcct gacgctcgcg gtggtcttcg ccctctcgcc ctatgcgttc cggtacggca
    22501 gcgagacccg catgtacgcg ctgctcatgg cggaggtctt cgccgggtac ctgctgctcg
    22561 acgcggcgat caccgcgccc cggtggaaga cgctggtcgg catctccacc gtgtcggggc
    22621 tgctgctctg gacgcactac tggtcgatgt ggctgctcgg cgcgatcggc gtgctggccc
    22681 tggtccgtct cgcccgcgcg cagcggcgcg gcgaccggcc cctcgtcgtc ggcaccgcca
    22741 agatcgtcgc gtccctcgcc gtcggaggcc tcacgttcct gccctggctg cccacgctgc
    22801 tctaccagag ccagcacacc ggcaccccgt gggcgccgag ctttcgtgtc acgaccctgg
    22861 tcgtcacgtc gatcaccgag ttcgccggag ggccgtactc cgaggcgcag ttgggcgtgt
    22921 tgctgctgac gatcctcgtg gcgatcggcg tgttcggcac cggcatcgac aaccagcgca
    22981 tcgaactcga cttcttcgcc cacccgctgg cgcgccgtcc gctgtcggtg ctggcgctga
    23041 cgatcgccat cgctgcggcg gtcggcatgg tgaacggcat ggcgttctca ccccgctacg
    23101 cagcagtgtt ctttccgttc ttcctcatcc tcgtggccct cggcctccag cagttcaagg
    23161 gagggctcgt ccgcgacgtt gtgctcgtga cgttcaccgc gttgtcgttg gcgggcatgt
    23221 tcctcgtggt ccgggtcgac cggacccagg cccgggcgtc ggtcgaggtc atcgagcgca
    23281 cggcccccga cggggtcgtc atcgcctgcc ccgaccagct cggtccgtcg gtgtcgcgct
    23341 acctcgaccg cgagcgctac gacatcacga cctacccacg attcgcgtcg cccgagctcg
    23401 tcgactgggt cgactacaag gaacgcaacg aacgcaacga cccggcagcg ttcgcccagg
    23461 agctgcttcg tcgtgccgag gggcgtccgc tgttcctcgt gtaccgcgac gacttcctca
    23521 cgttgaaggg tcagtgccag cgagtcatcg aaacgctgtc ggcggttcga ccgccccgga
    23581 ccatccagcg ggagtccggc gacgacttct acgagccgat gaccgtcgtg gagttcggcc
    23641 agcccggagc cggctgaccg tcgccgatcg accgtcgcgc ggcggtcacg ggatcatccg
    23701 gcccgaccac gccgccacgg cgtacccggc catgaggacc ccgctcatcg ggagcaggag
    23761 ccgatcgagc cagcgccacc gggccgggag gcgctgttgc gcgacggcga ccatcacggc
    23821 gagcgggaag gccgtgaacg cgtaccgacc gagcgagttg atgttgttgg cggcgagggc
    23881 aatgacgagc gtgactgcgg tgtagatcgt ccatccgacc gggagtcgcc gggcagcgag
    23941 gatgatcacc acgatggccc cgacggcgaa cgaggcgttg aagacgtcgc ggaactgacc
    24001 accggcaacg tcgaccaccg cggtcacgag gcgggacacg ggctcgtgcc acccggcgcg
    24061 gaagacccgc tgggagtcga gcggaacccg ccacgagtcg aaccgccact gcagccatgc
    24121 caggtaaccg accgtcccca cgccgggcgc cacgaccgcg gcgagatgga cgaccactcc
    24181 ccgtcggcgt cgcaggtcgt cgagcaccgc gacgaacagc gcgatcgcga cgagcgcacc
    24241 cgtcggccgc gtgagccccg acaacacacc gaggactatt gccggcgccc accgctcgcg
    24301 acggaccgcg agcaccgccc cgagtgcgag ggccagggcc agggactcgg agtaggcgaa
    24361 caccaacacc gaggcggcgg ggaacagcgc cagccaccac acgctcgcca cggcgagtcg
    24421 gcggtctccc gtgacgtcct gcacgagtac gtacacgaga accagcgcgg ccaacgcggc
    24481 caggttcgag accacgagga gcccgaggtc gtcgttgccg ccgaggagcc atccgaccca
    24541 gcgaccgagc agcgggaaga gcgggaagaa ccgctgcacc tcggtcgcca cgccgtcgta
    24601 gccgtgctgg gcgaggtcgc ggtagtaggt gccgtcccac aggaacagac cctggtgccg
    24661 gaccgaggtt gcggtctcga atcggtcgaa ccacgacatc gacgacgcgg cccacgcgac
    24721 ggcgaggagg acccgcgccg tgacccacgc cgcgagcgcg gtcaggtacg ggctggcggc
    24781 gacgcggtcg agtggacgtt ccacggcagt cacggcgtca gtcgccgagg tagctggcca
    24841 ccagcgtgtc gtggctcgcc gcgagctcgt ccgccggccc ctcgaacgtg acccgccccc
    24901 ggctgagcac cgtcgctcgg ctggcgacgc cgagcgccag cgcgacgtgc tgctcgacca
    24961 ggaggactcc acaccccgtg tcggcggcca gtcggccgat gacatcgagc agcgaggcca
    25021 ccacgagcgg tgcgaggccg aggctcatct cgtcgatcag gagcacgcgg ggccgactca
    25081 tgatggcgcg accgatggcc accatctgct gttcgcctcc cgacagcagg cccacgcgcc
    25141 gcgacagcag ccgttcgagg gccgggaacc agtccaggac acgcgccact gcgtcgacct
    25201 cgtcagcgcg ccgtcgggcc cgccgggagt cggcgaggcg gaggtggtcg cgcaccgtca
    25261 acgacgggaa gaggctgcgg tcctcgggga cgtgcgccac accggcacgg gcgatccggt
    25321 gcgtcgacga caacggcagc ggctcgccgt cgagcgcgac ggtgcccgac gtggcccgaa
    25381 ccaacccggt catcgcgccg agcgcggtgg acttgcctgc gccgttggcc ccgagcaggc
    25441 acacgacctc gccggtggcc accgtgaggt cgaggtcgtg gagcacggtc cggccgtcgt
    25501 agccggcgca caggcccgtg acctcgagga tcggtgcggc gctcacgtcg ccacccccag
    25561 gtaggcctcg atcaccttcg ggtcgttgcg gacctcagcg gccgtccccg ccgcgacgat
    25621 cgcgccgaag tccagcacgt acacccggtc gcacacctcg aagatgagtc gagtgtcgtg
    25681 gtcgatgagc agcgcgccga tgccggactc gtcgacgagc ctccggaggg tcacgccgaa
    25741 cgcgaggctc tcggtttggt cgagacctgc cgccggttcg tcgagcagga ggacccgagg
    25801 cccgcccgcg agtgcccgcg cgacaccggc cagcttctga tgaccgtgcg acagctccga
    25861 cgggcggcgg gcagcgtcgg ccgcgagccc caccgcggcg agggcgtcgt cgacgcgagc
    25921 atcggtggca gcgtcgtccg gacgatggag gaggtcgtgg aggacaccct tccaaccggg
    25981 ccgacgggcc gccaccaagc agttctgtcg gaccgtcagg tcgtcgaaga gctcgatgcc
    26041 ctgccaggtg cgcccgattc cggctcgggc gcgttcgtgg ggcgggagcg aactcaagtc
    26101 ggtgccgtcg agatgaaccg tcccgccgtg gcgtacgaac cccgagacgg cgtcgatcgt
    26161 cgtcgtcttg cccgctccgt tggcgccgat caggccgacc aactcgccgg cgccgacgtc
    26221 gagatcggcg cccaccacca cgacgttgct gccgtaccgg acggacaacc cggcagtgtg
    26281 cagcaccgtc agcccttgag caggctggcc agggcccacg cgctcacgag cactccgagc
    26341 agcaccaggg gtgcagcgcg gctgatcggg gccgcttccc gcctcggaga ggtcgggtcg
    26401 gccgggtcct cccgcggcgg gcggacgacc gcggcgacgt tggcgacggc gagcgcgccg
    26461 ccgaccgcca gcatcagcca cgcgagcagg tcgttgccca agaagagttg ctcggcggag
    26521 atcatctgac gcgatggcga actttctgct caaggtttcc ggggcggcgt tcgatacaca
    26581 ttgtgccagt ggaaatcgcc cccactggtg caaaacagcc ggggcttcag gagtggggga
    26641 cacactctcc ctccaaatgc atcgccacca acgctttcca gggtgttggc acgcaccgtg
    26701 gccctcgctc cgccccggac tgttctccct tcaacacaac acgcaagtca gctcatccct
    26761 ccccaagaag agctacgaac accgcccggg tttcgcccgg gcggtgtcgc gtgcgggaca
    26821 atgtgacatg gctgagtcaa cgacatcgac ggacactcac ggctccccgg cggcgcggac
    26881 caaggaccgt ccctggctga tgcgcacgta ctcggggcac agcacggcca aggcctcgaa
    26941 cgagctctat cgcaccaacc tggcgaaggg gcagaccggc ctctccattg cgttcgacct
    27001 gcccacccag accgggtacg accccgatca cgtcatggca cgcggcgagg tgggcaaggt
    27061 cggggtgccc gtggcgcacc tcggtcacat gcgtcagctg ctcgacggca tcccgcaggg
    27121 cgagatgaac acgtcgatga cgatcaatgc caccgccgct tggatgctcg gcctgtacgt
    27181 ggccaacgcg gaggatcacg gcgtcgattc caaggtgctg cgcggcacca cgcagaacga
    27241 catcgtgaag gagtacctct cccgcgggac gtacatcttc ccgcccgagg ccagccggcg
    27301 gttgatcgtc gacatgttcg cctactgcaa caagaacgtg ccgcactgga acccgatgaa
    27361 cgtgtgctcg taccacctgc aggaggcggg cgcgacgccg acgcaggaga tcgcgtactc
    27421 gctcgcgtgt gccatcggtg tgctcgacgc ggtgcgcgac tccggccagg tcgcccccga
    27481 cgccatgggc gaggtcgtcg cctccgtgtc cttcttcgtg aacgcgggca tccggttcat
    27541 cgaggagaac tgcaagatgc gagccttcac ccagatgtgg gaccgcctgt gcgaggagcg
    27601 ctacggcgtc accgacccga aggcccgacg cttccgctac ggcgtgcagg tcaacagcct
    27661 ggggctcacc gaggcgcagc ccgagaacaa catccagcgc atcgtgctcg aggcgttggg
    27721 cgtcaccctg tcgaagtcgg cccgtgcacg gtcactgcag ttgccggcgt ggaacgaggc
    27781 gctcggcctc ccccgcccgt gggatcagca gtggtcgctc cggatcatgc aggtgctcgc
    27841 gttcgagacc gacctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggat
    27961 cagcagtggt cgctccggat catgcaggtg ctcgcgttcg agaccgacct cctcgagtac
    28021 ggcgacatct tcgagggttc gaaggtcatc gaagggctga ccgccgagct cgtcgactcg
    28081 gcccaggccg aactcgacga catcctcgcc ctcggcgggg cgttcgaggc gatcgacgag
    28141 atgaagggcc ggctggtccg gagccacacc gaacggatgc gtcgcatcga gtccggcgag
    28201 cagatggtca tcggcgtcaa cgccttcacc gaaaccgccg aatcgccgct cggcggcgag
    28261 gacaacatct tgaaggtcga cccggcggtc caggccgcag cgatcgagga gctggccgag
    28321 tggaaggcca accgcgacca ggccgcggtc gacgccgcgc tcgacgagct cgagcgcgtc
    28381 gcccgcaccg acgagaacat catggcggcg tcgatcgccc tcgcccacgc cggcggcacg
    28441 accggcgagt gggccggccg cctgcgtgag gtgttcggcg agttccgcgc gcccaccggc
    28501 gtgggcgcag cgacgggagc cggcggtgcg tcgaccggcc tccgcgaact ggcagatcgg
    28561 ctgaaggaca cgcctggtgg tccgccccgt ttcctcgtcg ccaaacccgg cctcgacggc
    28621 cactccaacg gcgcggagca gatcgcggtc gccggccgcg acgccggcat ggaggtcatc
    28681 taccagggca tccgcctcac gcccgaacag atcgccgctg ccgcacgcga cgaggacgtc
    28741 gacatcgtcg gcctctcgat cctctccggc agtcacctcg aactcgtccc cgaggtcgtg
    28801 cgcctgctcc gtgccgaggg tgtgacctgc ccggtcgtgg ccggcggcat catccccgag
    28861 gacgaccgcc ctcgcctgct ggaggccggc gtcgccgcgg tcttcacgcc gaaggacttc
    28921 gagctcaccc ggctcatgac cacggtcgcc gatctggccc tcgaacaccg ctcggtcagc
    28981 gcctgaccgt cgacgctgcc aacccctcgt ccgttctcag gcggtgtcga gggaacggcg
    29041 agaccacccg ttaacttcag gagccatgac ccccgatcga gcaacggcgc cgcggcccgc
    29101 cggcggcacc caccgcgccg ccgcggtggc gctcgtcgcc tccatggcac tgctcgccgg
    29161 ctgcggcggc tccgccggca agatcacgtt gaaccggggg gattcgtccg acaccgctcc
    29221 cgaggtgtcc accgggcccg acggcacggc accgacgacc ggctccctca cctggggctc
    29281 gtgcagcaag ttcgacatcg ccgtcaccgc ggacgtgctc agctccgccc ggcaatccgg
    29341 cctggagtgc gccgctctcg acgttcccgt cgaccatgcc gcccctgacg gtgccaccac
    29401 cgagctcgcg ctggctcgcc tcccggcgac cggcagctcg tccaagcgca tcgggtcgct
    29461 catcgtgaac cccgggggcc ccgggggctc gggcctggag ttcctcgcat ccagcgggct
    29521 cgcgatcccc gacgacctcc gtcagcgctt cgacatcgtg tcgttcgacc ctcgcggtat
    29581 cgcatccagc tcccccctcg actgcctcac ccagacccag cgcgccgacc tcgtcgacgc
    29641 cgagtcaccc gacgacccgg cgcaggcaca ggcgcaggcc gccgacaccg agcggacgat
    29701 cgccgagggc tgcgaggcgg acgacgccga actcttccgg cacatgggca ccgaccaggt
    29761 tgcgggcgac ctcgacgaca tccgcgaggc ggtcggcgac gacgagctca ccttcctggg
    29821 cctgtcgtac ggcacccgga tcgccgcctc gtatgccacg cagttccccg acaaggtccg
    29881 cgccatcgtg ctcgacgggt cggtcacgcc gtcgcgtgac ctcgccgagt cgtcaggcgg
    29941 tcagatcaag ggcatcgtcc gggccctcga caacttcgtg cagctgtgca acgccgacac
    30001 gacctgcgcg atcgctcccg acgccctcgg caagttgacc gccgtcgccg actcgctcga
    30061 cgcgaccccc atgacgatcg acggtggcga cggcgaggag accctcgaca aggacaagta
    30121 cctgaccggt gtcgtcaccg ccctctatga cccgtcggtg tcgatggcgc tcgccgacgc
    30181 cgtggccgca ctgctcggca ccgattccga ccgggcgcag caggcaggcc ggttcctcct
    30241 cgatctcgcc ggccagcagg actcgaagca gcccgacggc acctacggca acggcttcga
    30301 gacgcaggga gtcgtcaact gcctcgacgc cgccgatccc ctcgacgcca gtgagctgcc
    30361 cctggtccgc gagatcgccg gccccatccc accactcctc gacagtgacc cggcgaccga
    30421 tacgccctcg tgcacactgc tccccaccgg cgaccggctc gagatcgccg cgaacgacgc
    30481 caaggaccgg ctcctcatcg ttggcaccga gggcgacccc gcgacaccga tcgagtggac
    30541 ccgagcgatg accgcggccc tcggcgaccc ggccgtgatc gtctacggcg gcagcggcca
    30601 caccgcgagc ctctcccgga agtgcgtcac gtcgcaggtc accgagttct tcatctccgg
    30661 caaggtgccc gcatcgctgc aggactgccc tcgggacccc gccgagagcg acatctacgc
    30721 ccagatcggc aaccagttcg aaacgatggg cctcggtgcc gacatcggta cctgcatcgc
    30781 caacgccctt cgcggcgaga tcgacccgct cgggatcatc tcgctcaatg gcgacgaccc
    30841 cgaccccgag gtcatctcga agctccagcg ggccgcactg tcctgtcgct gagcgtcgcc
    30901 ggatccgccg gtccgatgca caagaatgct tcacttgccg ccaggaatgc cgatggagtg
    30961 acgtgggatt gcgacgactg accggaatac tgctcggatg cgtgggcctg gccctcggga
    31021 tcgccggatt ggtggcggat ctgcccgtcc tcggcctcgt cggcggtatc gccgccgtgc
    31081 tcgccggcct ggtcgccttc ggggccgctg ccacactcgg tgacgccgaa gcccgcgcca
    31141 ccacggcaga gaccgagacg gcgacgcttc gccgcgagtt ggcgacggcc acgcagaacc
    31201 tgaacgagga gcgcacccag cacgacgcca cccgccagtc gctcgccgac tacatggccg
    31261 accagccggc ggcgggctcg ttcgaccgga agctgagtga caccgaggcg ccgctgggcc
    31321 agcgttccac caccccgagc ccgaccgaca cccgcctcct ggccgacccg gagacgaacc
    31381 tcttcagcga ggcgtacttc cgagtcgcgc tcgacgcccg gatcgcctcg gctcgccgcc
    31441 acctccgtcc ggtcgcggtc gctctcgtcg aggtcgtcga gggctccggc gctcccggct
    31501 cgccggaggc cgacgccacc aaggtgaccg gttcgatccg ccagacgctc cgcgacgccg
    31561 acactgcgtg ccgtatggcc gacgggtgct ttgccctgat cctcgaggac acgcccgaga
    31621 acggtgccgt gtggaccgtc gagcgcatcc gccgcaacct cacgagccgg ttcggtcagc
    31681 acacgatgtg ggccggcgtg gcgtgttacc ccgcccacgc cttctccacc gacgaactgc
    31741 tcgatcaggc gtcgctggcg ctcgagtcgg cgcgggagtg gaagcaggac cgcatcgagg
    31801 tcgcgacggc cgagtaggcc gtcgcccggc tcgccctctg gcgccggacg gcgtcaggac
    31861 ccctgcgtcg cgagtgactc cgcggccgcc acctcgaggt acgccgtgag caggcgcttg
    31921 gtctcgcgca ccgcggccgg atgggcacgg tggtcacgga ccgagaagtt cagcatcgag
    31981 taacaggtgt gcacgacgac ctgggcgatc gtcgaccgct cgcggcgatg ggtgcccggg
    32041 gtgagtggcg agatgatgcg ggcaacgcgg tcgccgagga tctgctggtg gcgggtcgac
    32101 gcctcgcggg tcgccggcgt cgcctgcatc gacagccaca cggcgcgacg cgacggatcg
    32161 tgactccact ccatcgcgag gtggtcgatc agcgcgtcca gcagttcctt ccactcgagc
    32221 gacgggatct gggccccgaa cgcatcgagc tcagcggcga cggactcggt gtcgagccgg
    32281 tcgagctcgc agacgatgga gaacttgttg gggaagaact ggtacaccgt cccgaccggc
    32341 acgtcggcgg cagcagcgat cgattcgcaa ctcagcgcct cgatgccggt ctcgaggagg
    32401 agggcccggg ttgcacggag gatgcgggcg aagcgctcac ggcttcgctc ctgcacgggg
    32461 cggcgacgag gggtgagatc gaggtcgtgg cgggtgagag gtgccgtacc gttcgccatg
    32521 aacgccattc tcgcagttcg acgcccagag gtggcgactg cgcggaggtg gattctcagg
    32581 gccagacgtt gaagccggga gggaggtcgg cccgtccgag catcgcccgc acgacggtgt
    32641 cgcgatcctc gaccgacgcg ctcgcgaaca cgagggcagc gacccgcacc ccggcgcgac
    32701 cgaggtcggg cgcgggacgc ccgagcgcca cacacaggtc ggacaggtgc accaccagtt
    32761 cgacgagtcg cgtgtcgagg taggccggca acagcatccc gccgacgacg gtcacgccga
    32821 tctcgtcgcc ggtcatctca ccgagccgac cgagcaatct gctgacgtcc tctacgacgc
    32881 tcgccaccgg gtcgtcgccg aggaggacgc cggcctcgcg gccccggtcg gcgacctgat
    32941 cgtggatgcc gggcgacgaa ccgagggcga tgcggaagta gtccggcgcc gtttccacct
    33001 cgacacgatc gaccggctcg tcgaggaacc ggtgcacggt cgagaacgct cgcatcgtgt
    33061 ggccgaccag ctcgcggacg ttccacacgc cgagccccgg ctccgaccat tcatcggacg
    33121 tgatcccgac gacggtgtcg acgaacgctg ccgacgcctc ggcgagcagc tcgacgatct
    33181 cgtcgtaggg caggggtgcg acgagggcag tcatgtcgcc accgcggtgc gggcccgccg
    33241 gtccgccatc gtgaaacaac gatcggcgag acgaccgaag gcgtacgcgc catagccgcc
    33301 ctcgtacatc tgacgggtgc gttcggacca gcgcagcgcc tcgggttcac tgattcgacg
    33361 gaacacctgg acgattcccc cgtcgtggat ccggtactcc gcgacggcgc cagggaagtc
    33421 cttcacgcac gccacctcga cgaacggcgc accgcccgcc tcggcaacgt gcatccgtcg
    33481 attgcggtgg gtgtggccgg ccaggtagcc ggtgaggttg tggcgccgag cgaagacctc
    33541 gaacagcgcc tcgctgtcgg cggggcggat cccgaagaag tcgtcgcggc gggggtcgag
    33601 ctcagggttc cacacgtggt ggtgcccgaa cacgagcacc tgggtatcgg tgcgggaggc
    33661 cagctcgtcg agccagtcga tctgatcggc agagatcgat ccgttgacct gaccgagccg
    33721 ggcggtgtcg agcacggcga gggtggcgcc ctcgaccacg acctcctgca tcgggaagtc
    33781 cgcgaacacc tgcccgtggt acgagtcgtg gttgccccga acatggacca gtcggtctcc
    33841 gaaggccgac ccgtacgcgt cgaggaaccg ctcgtactcg tcgtgtgtgc ccgtgctggt
    33901 caggtcgccc ttgacgacga ccacctcggg gtcgatgcgg ctgagctcgg cgatggcgcc
    33961 gcggttcatc acctcggggt agggctcctc gcccggggcc gtcgagaacg tcggaccgat
    34021 gtcggtcccg gagatcactc cgcactcggt ctcgccgaag tgaacatcgt tgacggtcgc
    34081 gacgcggccg aggaacgggc ccggctcgac cagcgtccgg acgtgctggt cgccgaattc
    34141 gtagacgcgg cccggttcga ggtcggcgta ggtccggagc tcccggccgt cgaacaggac
    34201 gatctcgtcg gtcgagacgg tggtgatctc cacagctggc aggctaccca ccgtccgtcg
    34261 ctttcagatg atccccgaga cctatccgcc acctcacggt gcgtggtggc gcaccccgaa
    34321 tcgaagagga tgccgggttc atcgtcgacc gggcgaggcg atcccgatcg gcgagccgac
    34381 ctggaagact gaccccatgg agacgatcct cgaggccatc gcccgcatcc gctcagccgg
    34441 gtacgacctg gagatgtccg ccactcccga ggggtggctg cagtgcggcg tgtgcaatga
    34501 ggtcgtcgac ccggcgaccg ccgtcgtcga cgagaccgtg cgcttcgaag gcgaatccaa
    34561 ccccgacgac gaggcgatcg tggtcgcgat ctcgacaccc cacggccacc agggctacat
    34621 cgtcgccgcc tacggcgccg acatggaggc cggcgacgtc gccctcctcc aggcgctcac
    34681 cgcccgctga ccactcgcac cggtcagcgg aggacgaact ggtcctcgtc gatgtcgacg
    34741 gtcaccttgg agccctccgc caccgttccg tcgagcagcg cgaccgccag gcggtcgccg
    34801 atctcgcgct ggatgagccg cttgagcgga cgagcaccga acagcgggtc gtaccccatc
    34861 gtggcgatct tcgacttcgc cgactccgtg acgtcgaggt ggatgcggcg gccggtcaaa
    34921 cgctcctgca gcagcccgag ctggatgtcg acgatgtgat ggaggtcgcc gggctcgagt
    34981 ggcttgaacc gcacgatgtc gtcgagccgg ttgatgaact ccggccggaa gaagtcatgg
    35041 ggatcgccgg gaaggttcga cgtcatgatc aggatcacgt tggtgaagtc gaccgtccgg
    35101 ccctgaccgt cggtgagccg accgtcgtcg agcagctgca acagcacgtt gaagacctcg
    35161 gggttggcct tctcgacctc gtccagcagg acgacagcgt agggccgccg acggaccgcc
    35221 tcggtgagct gaccaccctc gtcgtagccg acgtagccgg gaggagcgcc gatgaggcgc
    35281 gacacggagt gcttctccat gtactcgctc atgtcgatgc ggatcatcgc ccgctcgtca
    35341 tcgaacagga agtcggccag cgtgcgggcc agttcggtct tgccgacccc ggtcgggccg
    35401 aggaagagga acgaaccgat cgggcggtgc ggatcgctca gaccggcgcg gctgcggcgg
    35461 atcgcgttgg ccaccaccgt gacggcctcg tcctgtccga cgacccgctc gtggagcacc
    35521 tcctcgaggc ggagcagctt gcccatctca ccctcgatga gtcgggtgac ggggatgccg
    35581 gtccacttcg cgacgacctt ggcgatgtcg ctctcgtcga cctcctcctt gagcatctgg
    35641 tggtcggtct gcagttcggc cagttgcgcc gtcgccgctt cgacctcacg ctcgatcacc
    35701 ggaaggtgtc cgtaccggat ctccgaggcc cgggtgagat cgccctcgcg ttcggcacga
    35761 tcggcatcgg ccttcatcgt ctccaggcgc tccttcagtt cctggatgat ggcgatcgca
    35821 tccttctcgc cctgccagtg gcctcgcatc gagtcggcct gctcccggag gtcggcgagc
    35881 tccttgtcga tggcgagcag ccgctcgagg gacacggggt cgctctcctt gctgagcgcg
    35941 acccgctcca tgtcgagacc acggatgcgg cgctcgacca cgtcgatctc ggtcggcacc
    36001 gagtcgatct cgatgcggag cgcgctggcg gcctcgtcca tgaggtcgat cgccttgtcg
    36061 ggcaggaacc gcccggtgag gtaacggtcc gagagcaccg cggcagcgat cagggcgttg
    36121 tcctcgatgc gcacaccgtg gtgcacctcg tagcgctcct tgagcccccg cagaatcgcg
    36181 atcgagtcct cgacggtcgg ttcgccgacg tacacctgct ggaaccgccg ctcgagcgca
    36241 gggtccttct cgatgtactt gcggaactcg tcgagggtcg tcgcgccgac catccgcagc
    36301 tcaccgcggg cgagcatcgg cttgagcatg ttgcccgcgt ccatcgctcc ctcggagccg
    36361 ccgccggcac cgaccacggt gtgcatctcg tcgatgaagg tgatgatctc gccgttcgag
    36421 tcggtgatct ccttgagcac cgccttgagt cgctcctcga actcgccgcg gtacttggct
    36481 ccggcgacca tcgatgcgag gtcgagcgcg atgagccgct tgttgcggag cgactcggga
    36541 acgtcgccct cgatgatgcg ctgcgccagc ccctcgacga cggcggtctt gccgacgccg
    36601 ggctcgccga tgaggacggg gttgttcttg gtccgtcgac tcagcacctg cacgacgcga
    36661 cggatctcct cgtcgcgccc gatgaccgga tcgagcttgc cccggcgcgc cgcctcggtc
    36721 aggtcgcggc cgtacttctc gagggactgg tactgctctt cggggttctg cgacgtcacc
    36781 cggtggctgc cgcgcacctc ggcgagtgcc gcgagcacgt cttccttggt ggtgccgtcg
    36841 aggcgcttct cggcgggaga ccgctgctcg tcggcgatca ccaacgccag gaggaggtgc
    36901 tcggtcgaca ggtagtcgtc cttcagctca ccgcgttgct tggtcgcctc ctggaagatc
    36961 cccgtgagct ccttcgagat cgacgtgtcg gttccgtacg ccttggggag gcgcgccaac
    37021 gattcctcgg tgcggttacg gagcgatagg gggttcagcc cgatcttgcg gatgaccggc
    37081 aggacgacgc cattgtcctg ggagatcagc gtgacgagaa ggtggtcggg ggtcacctcg
    37141 gggttgtcgg acgtggacgc cagctcgatg gcggcgttca cggcctcctg cgtcttgagc
    37201 gtccaggtgt tcggatcgag actcatgttc gcctccttct tccttcgttg tagagcacga
    37261 ccgtggtcgg gggcacgggt acaaggtccc gtcggtactg gcgatgggtg tcggcgacgc
    37321 tggcggccgc ctcggcccgg gtgcgttcca gctcggcctc gagctgtttg atgcgccact
    37381 cgagctccag cacccgggtc actccggcga ggttgagacc ctcgttggtg agctgctgga
    37441 tccgctcgag gtgtgcgatg tcggcctggc tgtaccgacg gcttccaccg ccggtgcggg
    37501 ccgggttgag gaggcccttg cgctcgtaga tgcgaagggt ctgtgggtgg acgccggcca
    37561 gctccgctgc gacggagatg acatagaccg cctggtcgaa ctcgggcatg ggacctcctg
    37621 atggcttcgg ccacctccgc cgaggttcgc tcccgcgttc ctcgggagct gctcacgttt
    37681 cggagccctc agcgaatgga gtggattggt tggttggtca gacgcccagg tgggcgcggg
    37741 gtgactgggt ggaggcgtcg cggaacgcct cgagggcggt gcgttcgggg tcggagaggc
    37801 cggtcgggac gacgaggtcg acggcgacga gcagatcgcc cttgccggtg gcggtggcga
    37861 tgcctcgggc cttgacacgg aacacccgac cgttgggcgt tccggcgggg atgcggatgg
    37921 tgaccgcgtc gccgtcgagg gtcggcaccc ggatgtcggc gcccaatgcg gcctcggcga
    37981 aggagaccgg cacggtgagc agcaggtcat cgccctcacg accgaacagc ggatggggct
    38041 cgacgttgac acggacgtac aggtcgccgt cgggtgcacc gttgcggcca gggccgccct
    38101 tgcccttgag acggatctgt tggccgtcct tcacaccctc gggcagccga accttgacga
    38161 ctcgcgggcg ggtcatcacg ccactgccgc tgcacgccgg gcacggcgtg tcgatgaagc
    38221 tgccacgacc accgcagccg gcgcatggat ggctcaacga gaagaagccc tggttgtcgt
    38281 cgaccacccc gcgcccgccg cactcggtgc atacccgggg gctggtgccc cgagccgcgc
    38341 acgtgccgtt gcactcggta caggtggcct cgctgatcag gttgaccgac gtggtgacgc
    38401 ctcggatggc gtccatgaat gccaggtgca ggtcggcctc gaggtcggta cctcgctgct
    38461 gaccccggct gaagggcgca cgagcaccgc cctgctcgcc gcggccgaac aggttgccga
    38521 tgatgtcgcc caggtcggcg ccgttgaacg tggcgtcgcc ggggcgaccg aacccaccgc
    38581 cgggaccacc gccgctgagc cgcgagccca tcgggcccat cgcccgcacc tggtcgtact
    38641 ccttgcggcg ctcggggtcg cccacgacgt cgtacgccgc ggacacctcc ttgaacttct
    38701 cctcggcagt ggaatcgccg gggttcgcgt cgggatggaa ctgccgggcg agccggcggt
    38761 aggcggtggt gatgtccttg ggcgaggcgc tggcagcaac cccgagcgcg gcgtagtagt
    38821 ccttctcgaa ccactcacgc tgcatcgtca tgctcgatcg cctccatcgc ttcgcgaccg
    38881 gcgctccctc gagcgccggt ctgcttcgct gcggtctcgg gcgggcaagt cctaaccccg
    38941 caccttcacc atcgccggac ggaccacacg gccgttccag gcgtacccac ttcgcatgat
    39001 ctcggtcacg accggttcgt cgtcggcgcc gtcacccgct tcggtgagca cggcttcgtg
    39061 caccagcggg tcgaacgaga cgtcgacgtc gtcgaccttg gcgaggccac gcttctcgag
    39121 ggtgttcaac aacgccgact ggatcgggcc gacgtcctgg gcgccgtggc cgatcgcggc
    39181 gtcgcacgcg tcgagcaccg gcaggatctc gacgacgagc gcctcggcgg cgcgttggac
    39241 ctgttcgatc cgctgaccgt cgatccggcg cttgtagttc tcgaactcgg cctgcactcg
    39301 gcgggcgaga tcgaggtact cgtcgcgctc ggcagttacc cgctcgagat cctcgagggc
    39361 gctcgtgagg agctcctcgg ccgaaccggt ggagccgatc tggtcgagga gctcggcaag
    39421 gtcggtctca tcgaggtcga tgtcctcgac gagaccggaa tccaccgagt cgaccccggt
    39481 gtcggcggac ggatcgtccg ccgacacctc gtcgttggtg ggatcggtca cttctcgtcc
    39541 tcgatgatct cggcatcgac gacgtcgtcc tcggcgccac cggcggacgg gctgtcctgg
    39601 gtgtagccgc cgtcgttcgc tgcctgctcg tagagctgct gggcgaactt ctggctggcg
    39661 atcatgaggc cctcggtcgc gatcttgatc ttctcgatct cgccctcggc gatggcttcc
    39721 ttgagcgtgc cgagcgcctg ctcgacctgc acccggtcgt cgccctcgat cttctcgccc
    39781 tgctcacgga ggagcttctc ggtctggtac acgagcgtgt cggccttgtt gagttcctcg
    39841 gcgtcttcct tgcgcttgcg gtcctcttcg gcgtgcgcct cggcgtcctt caccatctgg
    39901 ctgatctgat ccttcgacag ggacgactgc ccggtgatgg tgatcgactg ctccttggac
    39961 gtcgcacggt ccttcgccgc cacgttcacg atgccgttgg cgtcgatgtc gaacgtgacc
    40021 tcgatctggg gcacaccacg gggggccggc ggcaggtcga cgagctggaa cttgccgagc
    40081 gtcttgttgt agctcgacat ctcgcgctca ccctgcagca cgtggatctc cacggacggc
    40141 tgcatgtcct cggccgtcgt gaacacctcg gtacgacggg tcgggatcgt ggtgttgcgc
    40201 tcgatgagct tggtcatgac accgcccttc gtctcgatgc cgagggagag gggcgtgacg
    40261 tcgagcagga ggatgtcctt gacgtcgccc ttgaggacgc cggcctggac ggccgcgccc
    40321 atggcgacga cctcgtcggg gttgacaccc ttgtggggct cacggcccgt catcgagtgc
    40381 acgagttcct gcaccgcggg catgcgggtc gagccgccga ccatgatcac gtggtcgagc
    40441 tggcccatcg tgaggccggc gtccttgagc gcctgctcga acggcacgcg gcagcggtcg
    40501 agcaggtcgg ccgtgatctc ctggaacttc gcccgggtca aggactcgtc gaggtggagc
    40561 ggaccggcgt cggtcgccgt gatgaacggc aggttgatct gggtctgctg gacctggctc
    40621 agctcgatct tggccttctc ggccgcttcc ttgaggcgct gcagagccat gttgtccttg
    40681 gacagatcga cgccgttgct gttcttgaac gagtcgacca gccagtcgat gacccgctgg
    40741 tcccagtcgt cgccaccgag ctcggtgtcg ccgtgggtgg acttgacctc gaacaccccg
    40801 tcgccgatct cgagggccga cacgtcgaag gtgccgccgc cgaggtcgaa cacgaggatc
    40861 gtctggtcgg cgccttcctt ctccatgccg taggcaaggg ccgcggcggt gggctcgttg
    40921 atgatgcgga gcacctcgag gcccgcgatc tgaccggcct ccttggtggc cgtccgctgg
    40981 gcgtcgtcga agtaggcggg cacggtgatg acggcctggg tgacggtgtc gcccaggtag
    41041 gcctcggcgt cgcgcttgag cttcatgagc gtgcgggccg agatctcctg gctcgtgtac
    41101 gccttgccgt cgatgtcgac ggaccagttc gtgcccatgt ggcgcttgac cgaccggatg
    41161 gtgcggtcgg ggttggtgat ggcctggcgc ttcgcgacct cgccgacgag cacctcgccg
    41221 tccttcgtga acgccacgac cgacggcgtc gtacgggcac cctcgctgtt ggggatgacg
    41281 acggggtcgc ccgcctcgag caccgcaacg accgagttgg tggttccgag atcgattccg
    41341 actgccttgg gcatgtgagc ctcctggtgc gaccaccgtg ggccgcggtt cgtgatgttc
    41401 gaccgccccc aggcggggac ggtgtattcg ctgatgacct cgactctacg gggccgaggg
    41461 cccaccagca ccttcgtggg atgaggcaag catattcctt gagtgtcctg ttatcaagtt
    41521 tgttgcggtg atggttgagc ccactcacag catcgggccc gggagccgcc gctgacgcca
    41581 ccgcctcacg aacgacggcg ggtgcgccga taggtcggtg tggacgacac atctcctcgc
    41641 cgacagtcgc cctcggggcc tgcgagcctc gccgtggttg ccgtgctcct cgcggccgcc
    41701 gacctcgccg ctcagtggaa catcgacggc aatcgacgaa tcgactaccg catctacgtc
    41761 cgcgccgtgt cgtcgatcga cggagccggg ctctacgact acggcaccgg catcctgcga
    41821 ttcacctacc caccggttgc cgcggtgctc gtctggccgt tcacgtggct cgacgaagcg
    41881 gtcgggtcga gggtgtggct gaccctgtcg gtcgtggcct tcgtcgtcgc ctctgcgttg
    41941 gcgatccaca acgccggagg cacccggccc gtccctccga cggtcgcgct cgttggcggc
    42001 gccgccgcgc tctggttggt gccggcgatc tcgacgctcc gactcggcca ggtcaacgcg
    42061 ttcgtcgcgc tgctcctctg cgtcgactcc gtcgccatcg cccgtcgctc ccgatggtcg
    42121 ggactcggga tcgggctcgc cgcagcgatc aaggtcacgc ccctcgccgt ccttccggtg
    42181 ctgtgggtcg gtctgcggcg ccacgaccac gtcgtggcac ggcgcgccgc gacgacgttc
    42241 gtggtggtca caggagcgac cgccgccgtc gccccgggag cgacctggga cttcgtgcag
    42301 cgactcggcg gtcgagccgt gacccgggcg gcgacgacgt cggtcgacct acgcgtgctc
    42361 ctccgatcgg tcatcgacgc acgcgccgtg gccgacgccg tctggctcgt ggccaccgtc
    42421 gtgctcgtcg ccgcggccct gcgcaccgct gcacggctgg ggcgttccga ccggcccgac
    42481 ggggtcggtc tggtcacggt cggcatgtgc ctgtcgaccg tggtgtcgcc gttcagctcg
    42541 gtgcatcacc tcacgttcgc gacgatcgcc ctcgtactgt gggcagcccg atccacctcg
    42601 tcgtggcagc gggccgtggc cgtcgccgga ctcctcgcac tgctcgaccc caccgccggc
    42661 gagggtgccc ccgggcgatg ggccatggtg gcgttctgca tcgcgacggt cgtcgcactg
    42721 ccgagcatcg tcgcgaagcg acccgacccg gatttcgtcg agaccgaccg aatcccccaa
    42781 gatgtgagcc tgctgcaagg aggccactga cgatgtccga tctgatcctg ctccgccacg
    42841 gcgagagcga gtggaaccgt ctcaacctgt tcaccggttg gtacgactgc gatctcaccg
    42901 acaccggccg cagcgaggcg gcggccgccg gctcgaccat cgccgacgcc gggttctcgc
    42961 ccaccgttct gcacacgtcg ttgcagaagc gggcgatccg cacggcacag ctcgcgctgg
    43021 acgagatcaa cctcctctgg ctcccggtcc gccggtcctg gcggctcaac gagcgccact
    43081 acggcgacct caccggcaag aacaaggccg agacggctgc gcagtacggc gaggaccagg
    43141 tgaagatctg gcgccgcagc tacgacgtcc cgccgccccc gatcgccgcc gacaacgcca
    43201 agaaccccaa cggtgatgcg cagtacgcgt ccctgccgcc cgacgtcatc cccggcaccg
    43261 agtgcctcca ggacgtcgtc acgcggatgc tcccctactg gtacgacgcc atcaccaccg
    43321 atcttgccgc cggccacacc gtgctggtcg tcgcccacgg caactcgctg cgcgccctcg
    43381 tgaagcacct cgacggtgtg agtgacgacg cgatcaccga gctcaacatc ccgaccggca
    43441 tcccgctgca ctacgagatc ggcgccgact tccggcccgt gacggccaag ccgaccgccg
    43501 accgctacct cggcgacgcc gaagcgatcg cggcgaaggc ggccgccgtg gccgcccagg
    43561 cgacggcgaa gtagtcggtc ccgccgtcgc cgcgctccac atctcgatcg gccagttcgc
    43621 cgccgtcgcg ttcatcgtca cgctcggcgc aaccgcccag ggcctcctcg ggttcgggct
    43681 gaacctcgtc gccgcgcccg tcgtcggcct cgtcgcgccg gaactcctgc cggcgaccat
    43741 ggtggtggtc ggtgtcccgc tctccaccat gatcgccgtg cgggaccgag agtcggtcga
    43801 ctgggcgggg gtccgctgga cgacgctcgg caggatcccc ggcaccatcg tgggcgcggt
    43861 ggtcgtgacg ctcgtgtcgg tccgcgcgct cggcgggatc gtcggcgcgg cagtgatcgt
    43921 ggcctgcgtc gtggccacgc gggccaacga gcaccagatc ggccggacca actccctcgc
    43981 cgccggaggg gcctccggat tcatggacac ggtcgcggcc accggaggcc caccattggc
    44041 gctgctctac cagcaccgcc cggcgccgga ggtgcgatcg accctggcga cgagcttcct
    44101 tgtcggcacc gcgatgtccc tggtcggact cctcgccggt ggcaacgtcg ccggctggca
    44161 gctcctggtc gccctcgcgc tcatcccgtt cctcgccatc ggcctggcac tgagccgacc
    44221 gctggcccac cggctcgcgg ggaggtcgct ccgaacggtg atcctgtact tcgccgccgc
    44281 ggccggtgcc gccgcgctca ttcgggcgct ggccggctga ccacgtacag ccctcggaac
    44341 ctcagacggc ggactcgcgg gacgtcaccg actgcgccaa ctggtacgca aggaagatca
    44401 gcgggagctg gaagggcacg cggatccagg cgacggcggc cgaatccatg ggcgggtcga
    44461 gacccttcat gccaccgtcg agggcggcct ggatgttggc gggccagacc ccgacgaacg
    44521 tgaggagcga gacgagcgcg ccgagacggc gggtcctcgg attgagcacg agcgctgcgg
    44581 ccacgagctc gacgacaccg ctcacgtacg tgacggtgcg gaccgatccg ggcatccact
    44641 tgggcacgat ctggtcgaag aactgcgggt tcacgaagtg catcgcgccg gcaccgccca
    44701 agatgccgac gagcgctgcg gtcgtgccca gattggagcc gcccgactcg gtcggcggtg
    44761 tcgtcgtgtc ggtcgtcatg gcggccaagt tagacgtccg cggcgcccgg caccccgcct
    44821 cccccggagc ccctggggca ggtagcgtgc acgaattcac atgcgcgctc gagtgcagtt
    44881 cccggaggaa gcagtgaccg acacccagac gtggtccacc accgtcgccg ccgccgcggt
    44941 cggcgccacc aaggtctacg gctcgggcga cacgcaggtc gtcgcgctcg atcacgtcag
    45001 cgccgagttc aacaagggcc agttcaccgc catcatgggc ccgtcgggct ccggcaagtc
    45061 cacgctgatg cactgcctcg ccgggctcga cctcctgacc gacggctcgg tcctgatcgg
    45121 cggcgacgac ctctcgaagc tcaaggaacg cgatctcacg ctgctccgac gcgacaagat
    45181 cggcttcatc ttccaggcgt tcaacctgat cccgaccctg tccgctctcg agaacatcac
    45241 gctcccgatg gacctgggcg gcatcaagcc cgaccagggc tggctcgaca cggtgatcga
    45301 caccgtcggc ctgcgcgacc ggttgtcaca ccgcccttcg gagctgtcgg gcgggcagca
    45361 gcagcgtgtg gcggtcgctc gcgccctggc gtcgcggccg cagatcatct tcgccgacga
    45421 gcccaccggc aacctcgact cgcggaccgg taccgagatc ctcacgttca tgcgcaaggc
    45481 ggtcgacgaa ttgcgccaga ccatcgtgat ggtgacccac gaccccaacg ccgccggcta
    45541 cgccgaccga gtcctcttcc tcgccgacgg caagctcgtc gacgagatgt acgaacccac
    45601 cgctgccaag gtcctcgaca agatgaaggc gctcggggac tgaccgagtg ctgagggtcg
    45661 cgctcaaatc cgtcctcgcg cgtaaacgac ggctcctcac caccgggttc gccatcgtcc
    45721 tgtcggtggc cttcatctcg ggcacgttgg tgatcaccgc gctcatgaac tcgacgctcg
    45781 actcgctgat cgggtcgtcg taccagggca tcgacgccat cgtccggagc gccaacgctc
    45841 aggagagcaa cttcggtcca ccgatccggc cgacggtccc ggacaccacg ctcgacctcg
    45901 cccgcgccac gaagggcgtg cgcgttgccg acggtgtcgt ccagggtctc ccgaccatca
    45961 tcgacaagaa cggcgaccgg atccaggatc tcgcgggtcc gccgaccatt gccttcaact
    46021 ggatcgatga cccggcgctg cagggcggca agctcgctcc aggcggccgg gcaccgaccg
    46081 ccccgaacga agtcgtcctc gacgtccgca cggcggaaga gttcggattc gaggtcggcg
    46141 accaggtgca ggccggcttc ccgtccggcc tcgaaacgct cgacatcgtc ggcatcggcg
    46201 gcatccccgt cgacggccag aaggagttgg tcgccgggcc gcgggtgctg ctcatgcagc
    46261 tcgaccgtct gcacgaactc ctcaagaagg acgggttcga ctacatcgcc gtgtcggccg
    46321 ccgagggcgt ctcccaggcc gagatcgcca cgaacctctc caagatcctc ccgcccgacc
    46381 aggaggtgct gaccggggag cagttcatcg ccgagagtga ggcgacgatc tcgaaactca
    46441 tctccctctt cacccaaccc atccttgcct tcggattcgt ggccgtgttc gtcggcgcct
    46501 tcgtcatcta caacaccttc tcgatcgtcg tcgcccaacg cacccgggaa ctcgcgctcc
    46561 tccgggcggt gggcgccggg cgtcgccaga tcctcggttc ggtgatggtc gaggccgtgg
    46621 tcgtcggcct cgtcggctcc gccgtcggca tcctcctcgg ctggggcctg tcgtacggct
    46681 tgaagcaggc gttgtccggt tcattcagcc tgccggcggg cctgcccccg ctgacgttgc
    46741 aggccgtgat cacgggcctc atcatcggca tcggctcgac gatgatctcg gcgttcatcc
    46801 ccgggctccg ggccaccttc atcccgcccg tcgccgcgct cggcgagacg gcgatcgacc
    46861 gcaccgcgct gaagacgagc cgaaaggtga tcggcgccct gatgctcctc gccggcatcg
    46921 ggctgatcgg cctcgtgtcc gccggcaccc tcgactgggg gctcgccggt cgcggcatcg
    46981 gggcgggcct cctcttcctc gcgatcacga cgctcggtcc ggtcttcgca ggaccgatgg
    47041 cgcgcacgct cggggcccca cttccccggg tgcgcggtgt gtcgggccgc atcggcaagg
    47101 agaacgccgg acgcaacccc aagcggaccg ccatcacgtc cgtggccctg agcatcggcg
    47161 tctgcctcgt cgtgatcgtc gcgatcttcg cggcgtcgat ccgctccacc gtgaagtcgc
    47221 agctcagcaa tcagctcgcc gacgtcgacc tggtggtcga ctccggcacc ggcttcgccg
    47281 ggctcggtcc cgagccgcgg cagttcttgg aggaccagcc cgaggtcgac ctcatcaacc
    47341 ccatccgctt caaccaggcg acgctgctcg acagcaaggg ggcgaaggag gagcaggcaa
    47401 cgcgcggcga aaccgacaac ggcgttcccg tcggcgagtc ggacttcgtc atcggcgtcg
    47461 accggaccgc catcttccag atcgccgcca tcgaggacct gacaccgaag atcaccgagt
    47521 tgcccgacga cgaggtgatg gtgctggcca agacggccga ggacaacggc tggaagccgg
    47581 gtgaccggat ccgggtgtgg ttccccaagt cgggcgagca gacctggaag atcgcagcga
    47641 cgttcggctc gcgtatcggc aacggcgcgg agtacatcac caacaacgag acgcagtcga
    47701 agaacgcctt gcccgagttc gacgtcgact cgaccatctg ggtcaagctc aacgacggcg
    47761 tcgacccgaa gaccgcgctc gccgagttca aaccggagct caagaagctc gctccgactg
    47821 ccggggtcaa cacgatcggc gactacgtgg gcgagcggct cggcatcctc gactcggtcg
    47881 tcaacctgat ctacgtgctg ctcggcctgt cgatcgtcat cgcgctcgtc ggcgtgggca
    47941 acaccatctc gctctccatc cacgaacgga cgcgtgaact cggcctgctc cgcgccgttg
    48001 gcatgacgcg ccgccaactg gccgaatcgg ttgtctggga gtcggtgatc atcgcccttg
    48061 cgggcaccgt catcggcctc gcgatcggcg tcgtcctcgg cacgatcttc gtggacggca
    48121 tcgaccagca gggcgtcgaa ccggtcgtga gcaccgtgac cgtcgcggtc atcggtgtgc
    48181 tcggcgccac agccggcgtg ttcctcgcca tccgcccagc gctccgggcg acccgcgtca
    48241 acgtgctcgc cgccatctcc tcggtctgac agagtgacgc ggtgaccgcc gagcagcagc
    48301 gacgggacga cgcctcgggc caactcgagg agtccgacga cgatcgcatc aggccgagcc
    48361 ccgtcgtggc gcggctcgcc ggagccatcg tcgacttcac gatcgtcgtc gtcctggtgc
    48421 tccccatcca gctgctctgg acgcactacg agagtttcga accggacgcc accaaggtgc
    48481 cgaccgggcc ggccaactgg ttgtcgctcg cgatcatcgt cggctacccg gtcatctcga
    48541 tcgcccggta cggccagacg atcggcaagc gggcgacgag cctgcgcgtc gtccgcgccg
    48601 acggccgacc agccggttgg ctggcatcga tcgtgcggtt cgccgtgtcg ttcgccccgt
    48661 tcctcaccgg gccgatcgtc cggcaggccc ccgccggtgc cggcagaacc gcgctcgagg
    48721 tgctccaggt gattctcatg gcggccgtgt acctgccgat ccccttcgac gaccagcggc
    48781 gcggcatcca cgaccgtgtg gcccgcaccc gggtcgtcac cgaactgccc ccgctgctgc
    48841 gcgcaccgat ctcggccgga cggggctgag ccgatgccca ccgccggcta ctccgggact
    48901 ccgctcccca agaagctcgg aatcaaggcc gggtgcgcgc tcgcgctcat cgatgcaccc
    48961 accggtttcg acgagacgct cggtctgacc ggcccgggcg cggcccagat gcccgacgac
    49021 gtcacggtca gccggtcgct cggcgaccgg cacgacgtcg tcgtggcctt cttcaccgat
    49081 cgacgcgacc tggtcgggcc tgcgcgtggt gtggcgccgc gagctccgga agcgttgagc
    49141 gcccccgccg tcagctgtag cgggcgtcgt ccatgttcca gtaggcgcgc atctcgctga
    49201 acttgccgtc ggcatcgaac gtcatcacgt cgatgatgtc gatctcgagg gtcatgtcgc
    49261 ccatcttcgt ctggacggtc atcgggaacg cggcgaagtt gccggcgcag cggatcgggc
    49321 ccgtgagggt cagcgtcagc tggtcgacca tctcgtgggt gcccgtgaag aaggcccgaa
    49381 ccgcttcggc gccgacgtgc ggatcggcgt cgaccggatc ccacgccgtg gcgtcggcgg
    49441 cgaagagctc gacgatgccg tcgacgtctc cggcggaatg gcgggccaga taggcctcga
    49501 cggctgactt catgtgcgct gcgtcggtca tggcggcgaa ccatagctcg gcatgccggg
    49561 cggaccggcc gccgagcagc cgccggtagg atctgtcccg ccgtgagcga caccaccaga
    49621 atcaccgagc tcgtgaccgg tctcggcatg ctcggcggcg atcacccgct cacgatgctg
    49681 cccgtcgcgc cgggcgagct tcgcaacgtc gacgccgcga cctgggacgg tctcgtcgag
    49741 agctggcacg accccgccct ccgacccctg gtcgatgccg ccttcgagaa cggccgcttc
    49801 ttcttcgagg catccgacgc gcttcgccga cggcgcccgc gcaccgtcga gtggaccggc
    49861 ccccagcgct cgcccggtga cgaggtcgcc cccgtcgatc tgcgcgtcga ccacgtctac
    49921 ctcgtcagct gcaagtacct gtcgaagatc acgatgaacg cctcgcccgc ccacgtcttc
    49981 gagcggctgc tcaccggggg ccacgggcgg cgcagcaacg tcgactggta ccgccacgtc
    50041 gccgcggccg aattcgaccg cctctggtcg gcgagcgccg cctggctgac ggaacaccac
    50101 gacggcgtcg ccgttcccga cgacccggcg acgctgtcga aacccgaccg caaggtcgtc
    50161 agcgaactcc tgcgcgacgg cggaaagtca tggccacaag agctgctcga cccctaccgg
    50221 gagttgtgcg ccgccgtctc cgttcgcagc gcgctcgcct ggcaggaatc gatctcgacc
    50281 gcgccgtcgg gagccgccga ggcgatgctc tggcgcctcc tccgtatcgg gagcgcgccg
    50341 tacttcgtgc tgggggtggg tggcgccgcc accgcgccgc tgcgccttcg cgtcgcgtcg
    50401 ccctgggact ggcgtcaaca cttcacgttc cggcgtctcg agatcacgcc gatgagcggg
    50461 gggcagccga tggtggggtg gcgggccgag ttcaccgaac gcgacggcgc ggcgagagag
    50521 gtccacggtc acgtggagat caggtggagc cacggccgct tcagcggacc accggaggcg
    50581 aaggtgtacc tcgacacccc gttcggcgac gtgcccggct actggtcgat cgcctgaccg
    50641 acctcgacgg tgtccggcgg cgacggcggt acctggcacc cgggacacca gtaggtgctg
    50701 cgcgcgtgca cgccgtggcg gcgcatccgt atcgaggcgc cgcaccggcg gcaccgctcg
    50761 ccggcgtgcc cgtacacggc gacaccgccg ggcacggtcg tgcgccgcgc tgtgtcgagg
    50821 ttggcccgta actgctgcgc cgcgatctgc accagctcgc gccgcagcga cacgggcacg
    50881 gcgtcgaccg gcgtgaacgg gtcgagccgg gccgcccagc agatctcgct cttgaacacg
    50941 ttgcccacac cacagcagat gcgctggtcg agcaacgctt ctgcaatcgt ggtcgtcggc
    51001 tcgaccagag cgaatcgggg aacgcaggcc tcgaggtcgg cgtcgggaag gcacaggtcc
    51061 ggtccgaggt gggcggtcgc cggcaggggc cgagactgcc cgatcgcgct cggcggtggt
    51121 ggcgtcacga actcgacgtc cggtgccgag aagcacaccg cctcccactc cggcgtcacg
    51181 agcagcaccc gaagctcacg acggggccga cgccaccgct cgcccgggcg atagatgtgc
    51241 caacttccag tcatccgcat gtgcgtccgg aggaccagac caccggagaa ccacacctcc
    51301 aggtgcttgc cgacggaccg cacgtcgagc accgtctccc cgaccgtcgg aagccgaccc
    51361 gtccaccgcg gtgcctcgac ccgatgcaac tcggcgccgg cgatcgccgc ccgcaatcga
    51421 gtcgcggagc gggcgatggt gtcgccttcg ggcatgatgt caccctatgc cgctcgatcc
    51481 ctcagaccca tccgcggagg tgttggagtt cctctccgaa cggcacctcg ccacgctcag
    51541 cacccaccgc cccgacggca cgatccacgt ggcaccggtc ggcttcacct acgaccccga
    51601 ccggcgactc gcccgcatca tcacccggtc tggttcccgc aaggtggcga acatcgacgc
    51661 ccaccccggg tcccgcgtgg tcctgtgcca ggtcgacggg ggccgttggc tgagcttgga
    51721 tggaccggcc gaggtgaccg ccgcatccga tcgcgttgcc gaggcggtcg aacggtacgc
    51781 ccaccggtac cggcagcccg gtgagcgcga cgaccgcgtg gcgatcgaga tctcggtcga
    51841 ccggatgatg ggtcggtgct gagcgccgct cgctcgaggg cctagagcgg ggagctcccc
    51901 ccgccaccac cggcaggctc gcgcaacgcc atctcggtga cgccgtcggg aaccgtccgg
    51961 gtgcccgtca ccccggtgag cgccgccggt ccggttgtcg cgagctggct cagcgcaacc
    52021 tgccggccga ccctcacggc gacccacgcg ccgtcggtga ccgcgtcatc ggacgccggg
    52081 gcgttcgccg gttggatgtc gacggtcgtg gcagcgccgc ccgggaggtc gagcgtcccg
    52141 accttgacct cgccgctcat gggcaccgat tcgatccagg tgaggcgggc accggtggcg
    52201 tcgagggcca gcgacagcac ggttgcggca tcgacgtcgg gcacgggcgg cagatcgctc
    52261 ggatcctcgg tggtcgacaa cgtgcaggac tcgccctcgc cgaagcacac cgcgaaccgt
    52321 ccgttctcga cgtcggcggc gaaggggccg ctctcccagc cgtaccaccc gtcgagcggt
    52381 gtcacctgct gagtgtccat gtcgatcaga cgggcgacga gaccgctgcg atcctcctcg
    52441 aagctcggca cgacggtcgt gtcggtccag cccgtcagca gcacgcgatg gtcctggtag
    52501 gtgccgatcg ccatcagatt gtcggcttcg ccgagcgcca acggcgacac acctttcaat
    52561 tgctggtcct cgaccacaca cggcaaggtg tcgccggtgc accggaggta gacgacgccg
    52621 ccgaccccgt cggacagcgg ggagcgaagg gtctcacccg gccgcggctt ggcccacacc
    52681 tcaccggtct cgtcgacgat gccccccgac gtcgccaacc acagacccgg gagctcggca
    52741 cctggctccg gcaccgaggt cgggccgggg tcgatcgtgg tcgtggtcgt ggtctccggg
    52801 gcgatgcccg ttgtggtctc ggagggggag atggcgtcgt cggaggagca gctcgccagc
    52861 agtgagacgg cggccaccgc tgcaagcgcc gcgagcacca ccgtcgctgg gcgggaattc
    52921 gatcgcatca ggacatcctc gcgaacgtcg ggcacgagtc cggcgcggac gaccagatcg
    52981 tcaacagact gcaacgtcgc ggcgccggtt cacctcacga acgaaggatc aacccgcgat
    53041 acccgtcgag gaagccggcc tcgcgcagag gatctgccca gctcgactcg gacacgggtg
    53101 ccccgtcgac ggtcgtgatc tcgagccgcc gtacccggcc cgagcgcacc agaccggcca
    53161 ctgcggccac ccactcgggg tcattgccgg ccgcggggaa cgtgacgaga ttgcggcccg
    53221 accgatcgag ccaggcgacg ggatgaccgt cggccagcac cacccgggcg ccggcggacc
    53281 gggtcgggcg gccggcgttg tcgggccacg gcagcgcagc accgtagggc tgcgccggat
    53341 cggtggcggc caggacgaac ggcggacccg tcggctccga ggctgcgccg aacgccgacc
    53401 cgacggcggc atcggacccc gtgccgtcgg agacggagcg gaactggcgg agtcggtcga
    53461 cggcggcatg ggtcgcgaac tgcgctgcgc ccagcccggc gacgaagtaa ccacggcgga
    53521 tctggccctg ttcctccatc gccttgagca ccgggtagac accggcgaag ccggcgacga
    53581 tgccctcggc gagggccatc tcccgggtca gcacgccgta gcggtcgagg agttgcagcg
    53641 tctgcgcgtg cgacacctcc gtcgaggtcg gcggcaccgg caccaggtcg gccacgagcg
    53701 accaccggcc ggcccccgcc gggggcgcag cgctcatcga tcgcaacccc cgggaccggg
    53761 tgcgccgcgg tgacgaacgc ttggacgtcg acacgacctt ggcccggagc ggggcgaacg
    53821 agtcgttcgt gacctcacca gcccagacga gatcccacaa cgcgtcgagc accgtggcgt
    53881 cgtcatacgg caggtcggcc gctgcgacgg cggcgagcag gtcggtccag aacgatgcac
    53941 ctcggtcgcg gagccgctcg cggatggcca catggtgcgg atctccggga gcggcctcgg
    54001 cgtcgatcga cgacagcgct gcgctgagca aggccgtgtc ggagcggaag acgaggcgga
    54061 cccggccgtc cttggtgccg atccctccgg ctccgaccca gaccagttca ccagcggtgc
    54121 acatcgcgtc gaggtcggcc ggccggtagt cgccgaggcg cggcgcgagg acttcgacgt
    54181 cgagtgacga cgcgacgagt ggcgcaccgg ccaattggga cacgacgtca gcgagggcct
    54241 cgggcccctt ggctgtcgaa ccgacccgct gccaggccgg gaggaaccgc cccagcactt
    54301 cgggatcgac ggcctccacg tcgcgtcgca gcgccgccag ggtgcggcgc cggatcatgc
    54361 gcaacacctc gggatcgcag aactccgtgc cggacccacc cggacggaac tctccacgca
    54421 gcacccgttc gtcggcctcg agcgacgcaa gcgccaactg cgccgtctcg accgggacgc
    54481 cgagccggtg ggcgacggcc gcggcggtga acgggccgtg ggtccgggag taccgctcca
    54541 cgagcgatcg cagcggtgcg tcgttggatt cggtgtaggc agcgggcagc cccacgggca
    54601 gcgcgacgcc gagggcgtcg cggaggcgcc cggcatcggc tgcgtcggcg atctcacggc
    54661 gccccgccac cgtgacctcg atggcccggc gctggtcggt cagcgtcgat acccactcgt
    54721 cgaccaccga caccgcaacc tcggaccgta cggcgagctc atcggtcggg agggggccga
    54781 gcacccgcaa caggtcgtgc agctcctcgg cgctccgcgc ccgccgcccc tcggcaaggt
    54841 gctggagctc ggattcgatc tggtcgatga cgtcgccgtc gagcagcgac cgcaactcgt
    54901 cgctgcccag cagctcgcgc aggaggttgc ggtcgagacc cagcgcggcg gcgcggcgct
    54961 cggccagcgg cgcgtcgccc tcgtacatgt agaccgcgat ccactcgtag aggagcgacg
    55021 cggcgaaggg cgacgcgacc ggggtgtcca cgctgacgac ccgcacccgg cggtcgcgca
    55081 ggcgtgtcat caggtcgatg agcgccggca ggtcgaacac gtcgttgacg cactcgcgcg
    55141 tggtctcgag caggatcggg aacgtcgggt accgggcggc gacggtcagg aggttgcccg
    55201 cccgctggcg ctgctgccac agcggtgtgc gctggtcgac acggcgacgc ggcaacaaca
    55261 gggcgcgcgc cgcggcctca cggaaccggg cggcgaacat cgccgagttc ggcagcatcg
    55321 acacgaccag ctcggtcacg tcgtcgggat cgatcaggag gtcgtcgatg tcgagggcgt
    55381 cttcggactc ggggaggcgg aagatgatgc cgtcgtcgga ccacatgatc tcgacgtcgg
    55441 tggcgccggc cgcctcgagg cggccacgga tggccatgcc ccacggggcg tgcacccggg
    55501 caccgaacgg cgtcagcacg cagatgcgcc agtcgccgat ctcgtcgcgg aatcgctcga
    55561 ccacgatcgt ccggtcgtcg gggacgacac cggtcgcctc cgcctgctcc tccaagaacg
    55621 cgagcaggtt ggtggcggca cgctcgtcga ggtggtggcg ttcgccgagg gtcaccaacg
    55681 cctcggcagg cgggaccgaa cggatcgtac gggtgaactc accgatcgca cggccgagct
    55741 cgatcggccg accgggaccg tcaccgtgcc agaacggcat cttgccgggg agaccgggcg
    55801 ccggggtcac gatcacccgc tcgaacgtga tgtcctcgat gcgccacgtc gtggcgccga
    55861 gaaggaacgt ctcgccgacc cggctctcgt agaccatctc ctcgtcgagc tcacccaccc
    55921 gcgcgccgtc ggggaggaac acgccgaaca gcccacggtc ggggatggtg ccggcgttgg
    55981 tgacggcgag acgctgcgca ccggcacggc cacggatgac gtcggcggta cggtcccaca
    56041 cgatgcgggg gcgcagttcg gcgaactcgt cggaggggta ccgaccgtcg aggaggtcga
    56101 gggttgcggt gagcaggtcg gccgagagct cgccgaacgg cgccgaggcc cgcaccgcag
    56161 cgcgcagctc cggcagcgtc cactcgtcga gcgccgacat cgcgacgatg tgctgggcca
    56221 acacgtcgag tgggttgcgg atgaagcggg tcgtctcgat ctcgccggcg tccatccgct
    56281 gcacgacgac ggccgtctcg accaggtcgg cgcggtgctt cgggaagaac cgaccccggc
    56341 tctcggcgcc cacctgatgg ccggcgcgac ccacgcgctg caacccactg gcgaccgagc
    56401 cgggcgactc gacctggatg accaggtcga ccgctcccat gtcgatgccg agttcgagcg
    56461 agctcgtggc cacgaggccc cgcaggcgac ccgacttgag atcgtcctcg atcacgagcc
    56521 gctgttcgcg gctgagcgag ccgtggtggg cgcggacgag ttcgcctccc gccggtgggc
    56581 tgccgacgtt ctctgcgccc cgcgagggtt gctcgtcggc cagctcgttg agtcggctcg
    56641 acagacgctc ggccagtcgg cgggcgttgg cgaagatgat ggtcgagcgg tgttcgagga
    56701 tcagctcgag caggcggggg tgcatggcgg gccagatgct gcgccgcacc ggcccggcag
    56761 acgtggggcc gctcggcagg tcaccaccgg actcgtcgat gatctcgccg agcgccccca
    56821 tgtcgtccac cggaacgatg acctgaatgt cgaggggctt gcggacgccg gcgtcgacga
    56881 tcgtcaccgg ccgggggccg accacgtcgc ccgagcgcgg atcgaccacg ttgccgccga
    56941 ggaagtgtgc gatctcgctg agcggccgtt gtgtggcgga cagcccgatg cgttggggtg
    57001 accggtcggc gaccacgtcg aggcgctcga ggctgaccgc caggtgcgcc ccgcgcttgg
    57061 tggcggccac ggcgtggatc tcgtcgatga tcaccgtggt gacggaacgc agcgtctcac
    57121 gcgccgcgct cgtgagcatg aggtacagcg actcgggggt cgtgatcagg atgtcggggg
    57181 gatgggcgac gaggcggcga cgatcctgcg ccgaggtgtc gcccgagcgg accccgacgg
    57241 tcggttcgcg gaacggcacg ggtggggcgt cacccgacga cggatccgtc gccaaccgct
    57301 gggcggcgag catcgacccc cggatcggtg cacgcaggtt cttctcgacg tcgacggcga
    57361 gtgcccgcaa cggtgagatg tacaagacgg tggtggactc gaccacgttg cccttgtcgt
    57421 cgagccggat cggggcggtc gcgagcgcgt cgatcgccga caggaacgcg gcgagggtct
    57481 tgcccgagcc cgtcggcgcg cacaacagca catgcgcgcc ggaggcgatc gccggccacg
    57541 cctcggcctg ggcccgcgtg gcctcgccga acgtcgtgcg gaaccatgcc gccgtcggtt
    57601 cgctgaaacc tgcctcgtcc aggccggggg tcgccatggg cgcaggctac ccagggccgg
    57661 tgacagtgag ccctgccggc gacctcgggg tcgcccggcc acgtacaatc ccgccatggc
    57721 agtccccacc acgtccaagt cgcacaagtc cggggagttc cacccggcgt tcgaggagta
    57781 ctgcgaagcg atcttcgaat tgcaggaaga cgcggtcaac gtcatccagg cgcgcatcgc
    57841 cgaccgtctc gagatctccc gccccgccgt gtcggagatg atccgccgca tggccgccga
    57901 agggctgatc accgtcgacg agaccatcaa gctcacaccg gcgggccagt cgctcgccga
    57961 ggccgtcgtc cgccgtcacc gcctggccga gcgactgctc accgacgtgt tgggcctgtc
    58021 gtggtcgacc gctcacgccg aggcgggcaa gtgggaacac gtcatcagcg ccgaggtcga
    58081 ggcggcgctg gcccgggtgc tcggcgaacc caccacctgc ccccacggca acccgatccc
    58141 cggttccgac tacgaggcac ccgacaccgt tgcgctgagc cggttgcccc gtggtgccga
    58201 gttcacggtg agtcgcatcc ccgaagagct cgagttcgcc gacggcatgc tcgacttcct
    58261 cgaggacaac ctgctgcgtc cggggttcga gggcgtcgtc gacgagctgg tcccctccgg
    58321 ggaacggatc gtcaccatcg acggccagac cgtcaccatc ggtcagttcg cgagcgagcg
    58381 catcctcgtg agcgtcgcgt gagcgcccac gaggacggca cgcccgtcgt cgtcaccacc
    58441 ggagcgggcg cgctccgcgg ccgttggcag gacggggtcg cacgctttgc cggggtgccg
    58501 tacgcagcac cacccgtcgg cgatctccgg ttccgtgcgc cggcgccggt cgaaccctgg
    58561 accggcgagc ggtccgccga ggcgttcggc ccgatctgtc cccagaaccc gtcgatgatg
    58621 gatgccctgt tcggcggcga ttccgaacgg tgggacgagg actgcctgta cctcaacgtg
    58681 tggacacccg accccgaggc ggccgccacc ggcccgctgc cggtcatggt gtggatccac
    58741 ggtggtgggt tcgagatggg ttcgggttcg tcaccgctct acgacggcac ccacttcgcc
    58801 agctccggcg tggtgctcgt ctcgctcaac taccgactcg gcgcattcgg gttcctcgag
    58861 ctcggccacc tcgacgccga cctgtccggg tccggcaacg ccgggctgct cgaccagatc
    58921 gctgcgctcg agtgggtgca gtcgaacatc gccaacttcg gtggcgacgc tgccaacgtc
    58981 accgtgttcg ggcagtcggc gggatccatg agcatctcgt tgctcatggc gtcgccgcgg
    59041 gcgaccgggc tgttccgccg ggccatcacc cagtccggtg cggccaacgc cgctcgcgcc
    59101 gtcgaatcgg cgcagggcga cacccacgac ttcctcgccg ccggcggctg gtcgtccgcc
    59161 gccgacgtcc gggcggcacc gaccgccgac ctcctcgctg cccacgcatc gttgtcgggc
    59221 gcgcgggtcg ccgaccccga ggccttcatc cgtcgtgccg ggacaccgct cgggttcttg
    59281 gcgttccgcc ccgtggccga cggcgtcgtc gtgccgtcgg acccgctcgc cgccatcgcc
    59341 gccggctctg ccgccggcgt gacgctcctc gccgggtcga cctcccagga gtggcgactc
    59401 ttcgccctca tgacaccgag cccggcggac gaggacgcgc tccgcgatcg gttcgcgctg
    59461 ctcgtcgacg atcccgatgc cgcgctcgcc gcctaccgcg acgagtatcc cggcgcgtcg
    59521 ctcgccgacc tcgatggagc gctgctcacc gacatggtgt tccgcatccc gacggtccgc
    59581 ctggtcgacg cccagtcggc gcacgctccg acctttcagt acctctggga ctggaagagc
    59641 gacgcgttcg gcgggatgat cggcgccgca cacgcgatcg acatcccctt cgtcttcgac
    59701 ctcctggagg accaccggct gcacgtgttc gtcggcgccg acgcgccgcg gtcgctcgct
    59761 cgtgccacga acgaggcctg gatcgcgttc gcctcgaccg gcgtccc
//
LOCUS       DAOSOA010000002        44724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_887_length_44724_cov_6.914639, whole genome shotgun sequence.
ACCESSION   DAOSOA010000002 DAOSOA010000000
VERSION     DAOSOA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 44724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44724
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_44724_cov_6.914639"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKY13_00275"
     CDS             complement(<1..385)
                     /locus_tag="PKY13_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT78383.1"
                     /translation="MTAPLQAHRDGTTTLHDGRELGWAIFGDPEGDTVFWFHGTPGAR
                     SQLPHDAGTLAVERGLRIVTVERPGTGDSTPHVYEQIVEFAADLRQVADHLDADEFAV
                     VGLSGGGPYLLAAAHELGDRMRAGVV"
     gene            505..1860
                     /gene="cysS"
                     /locus_tag="PKY13_00280"
     CDS             505..1860
                     /gene="cysS"
                     /locus_tag="PKY13_00280"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT78384.1"
                     /translation="MLRLFDTAAGAVRPFEPRVPGQVSMYVCGPTVYGPPHLGHGRFS
                     LVFDVLRRYFEWSGLEVTYVSNITDIDDKIIERANSEGRPWQEITEKCEAVWYRAMDA
                     IGVKRPTHDPHATAYVTQMVDLISRLIEKGAAYETSDGVYFRPRQVADYGLLARQDID
                     SLQVGARVDVVDEKETPIDFALWKKAKPGEPSWPSPWGDGRPGWHTECVVMSLDLLGE
                     GFDLHGGGQDLCFPHHENERAQAVADGKDFAHHWVHNGFVEVGGEKMSKSLGNFTNLL
                     DLIDRYDPRAYRLLCLQAHYRSPIDVNEKNSAQASAALERYDALARRVTDLGHGVAPD
                     PVIIGRFRDRMDDDLNTGGALAIVAETVTQINRSLDIDDFAGAEPLVAAFRSMLSAVG
                     LTVNDEVAAVADEAADLCRQRDDARAAKEWGRADALRGELEAMGYVVEDTATGTQVRR
                     A"
     gene            1889..2842
                     /locus_tag="PKY13_00285"
     CDS             1889..2842
                     /locus_tag="PKY13_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOT78385.1"
                     /translation="MALTVVALGCSGEDDSGSAGGALSSSRRDGTTSTSSTTVAPPAP
                     RSTVVIAHRGASAYAPEHTFASYDLALEQGADYIEQDLQMTADGQLVVLHDPILDRTA
                     RGPADACTGPVASKTLAQLSACDVGSWFNEAHPDRADPAFAQLRIPTLREVLERYAGR
                     ARFYIETKSPEAQPGMEQALLDVLDEVGPPADGTSAAGERSVIIQSFSAESLRLVHSL
                     RPELPLVLLLVTSGEPLDPAILDGVGDYAVGVGPSSGNVDEALVDAAHSQCLDVHPYT
                     VDDPAEMTRLIAAGVDGLFTNAPDVLRPLVAALPGGRDACG"
     gene            complement(2866..3621)
                     /locus_tag="PKY13_00290"
     CDS             complement(2866..3621)
                     /locus_tag="PKY13_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOT78386.1"
                     /translation="MTDTNTTTGAAATSATIDIRRADTRPRTELGWLDGRHSFSFGSH
                     YDPTNLGHGLLIVSNDDRVAPGGGFGTHPHRDMEIVTWVLDGQLEHRDSEGNVGLLYP
                     GLAQRMSAGTGIQHSEMNASRSVPVHLVQMWVVPAETGIRPGYEQLDVNERLDAGGLV
                     PVASGRGHEGAITIHQPGAVMWVGRLGDGETVALPDAPYVHLFVARGSVSLGGDTLVE
                     GDAARLTAAGAVDVTGVGDAEVIVWESDSDSGR"
     gene            3719..4177
                     /locus_tag="PKY13_00295"
     CDS             3719..4177
                     /locus_tag="PKY13_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT78387.1"
                     /translation="MATEREPHPLQLEAWRSFLEAHARVTELLDAELRAQRDLPLGWY
                     DVLVQLSEAGGRRRMSELADAVLLSRSNCTRLVDRLDAAGLVGREPDPADARVRWAVL
                     TEAGRARLKDASSAHLAGVERAFTSFVDDDAAKVLVSVFHPIIDALRAQR"
     gene            complement(4209..5696)
                     /locus_tag="PKY13_00300"
     CDS             complement(4209..5696)
                     /locus_tag="PKY13_00300"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOT78388.1"
                     /translation="MHRRRPAVVLAVALATTLIGGCSDDPGRITARVDRSTTTTTAAA
                     TTTTAAPTGTAGSADVGDAIVPGAGNGGYDVTNYDLAITVADDRTTIEGTATIEVTAT
                     QDLSAFNLDLEGLTVDDVTVDDEAATFTHEGSELTITPTSPIASGADLTTVVQYHGAP
                     TPVQDPSAPGTIGWLSAPSGTYVAGEPTGARGFFPCNDHPSDKATFTIAVTAAERDTV
                     VANGVLGSKAATGDGHTTWTFGQSDPMATYLVQIAVGDYDIVESTGPGGLPIRHAVVR
                     SVTADQREVLDDTEAQLEFFESVFGPFPFDTYGLLVADSGPEFALETQTLTLLPSEWL
                     TSFDPTSTSSVMAHELAHQWFGDAVTPARWSDIWLNEGFATYGEWLWNDHIGTTPIDD
                     AVDDAMAQAPRWREAFGPVTSPAAPVLFSPNEYDGAGIVLHALRLTVGDDVFFEILRT
                     WASERDGTSATTEDFQELATRLAGQDLVPFFDAWLRSPTLPTMPR"
     gene            5896..7113
                     /locus_tag="PKY13_00305"
     CDS             5896..7113
                     /locus_tag="PKY13_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT78389.1"
                     /translation="MTDFSLSLNEDQLQLKEWINGFAKDVMRPAAEEWDEREEFPWPV
                     VQEAAKIGLYGMDFMAQGIADPTGLTMVVAIEELFWGDAGIGLSLMGSGLAAAAIAAN
                     GTPEQMFEWVPQCYGTADKVQLGALCASEPDAGSDVGGYRTRATYDEAKDEWTLNGTK
                     AWITNGGIADVHVVVAVVEPGIGSRGHASFVVPPGTIGLSQGQKYKKHGIRASHTSEV
                     VLEDVKIPGSCLLGTKERLDAKIAKAKENVSTGEKQPAMATFEATRPTVGAMAVGIAR
                     AAYEYSLQYAKDRQAFGEPIIMKQGVAFKLADMATEIDAARLLVWRAAWMSAAKVPFK
                     QGEGSMSKLKASEVAVKATEDAIQILGGYGYTREYPVERWHRDSKIMTIFEGTSEIQR
                     LVIARAASGLRIP"
     gene            7242..8210
                     /locus_tag="PKY13_00310"
     CDS             7242..8210
                     /locus_tag="PKY13_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT78390.1"
                     /translation="MPLIQAEQLTKSFGDTKALAGLDLEVEQGTVLGVLGPNGAGKTT
                     AVRILTTLLRPDGGRATIDGIDVAKEPRRIRSRIGLTGQYAAVDERLTARENLELIGR
                     FFRMSGGDSKRRAQELLDRFRLDQAGDRVVSGFSGGMRRRLDISMSLVARPTVLFLDE
                     PTTGLDPRSRNAMWDLIDELTAEGMTTLLTTQYLEEADRLAKEIVVIDRGQAIAKGTA
                     DELKDLLGGARAEVALGEGADQGAVIAGLGGLVNPDAEPAVGAGTVTVPLRQGTTTPQ
                     VVRALDAAGVAVRDVTVSRPTLDDVFLTLTGHAAEEVAADDEGDQS"
     gene            8207..9061
                     /locus_tag="PKY13_00315"
     CDS             8207..9061
                     /locus_tag="PKY13_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT78391.1"
                     /translation="MTATSPNATSPSSASSLVGRAGDTVDAPGGVVWALRDSWNEATR
                     HLRAVPRSPELLLFATLQPIMFVLLFNYVFGGSVNVGGDYTQFLIPGVFAQTVVFGSA
                     FTGIGIAEDLQKGFIDRLRSLPISQGAVLIGRTMSDMVRNVLTFLVMLGVSFLIGFRF
                     EGGLAKGLLATALLLGFSYSFSWIQALIGLSVKSVEAANSAGFIWMFPLTFLSSAFVK
                     PENMTSAWLRKVAEWNPFTIVTNAARALYGGTPVGDTVWQSIVFAIAITVVFSFLAVR
                     KFKSAASH"
     gene            9251..10195
                     /locus_tag="PKY13_00320"
     CDS             9251..10195
                     /locus_tag="PKY13_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78392.1"
                     /translation="MDFGPIHERASTQRALITFDQLLDAGVSRHQVDRLSAQGFLVPI
                     HRGVFRLPGSPLSWEQRVVAAALATAPDSWISGPRALAWWGVRDGPSVEVLTTSARRV
                     RLEGVRAHQTIDLPGVDRRRHRDVPVTSIERSLFDSGRSLTHQQVGAALDHAVRDGLT
                     TYERHQQRVEELGRRGRAGSATAQRVLTARGYGDGFGFEKAMRGLLREVGLPAPVREL
                     KVHVDGFRYRVDFAYPDSMLGIECDSGEWHGLFHQVECDLERQNRIQKEGLLLMRYTV
                     TRLRDERDAVGTEIVEAIARRAGSTPERPWVFPDVKPA"
     gene            complement(10263..11606)
                     /locus_tag="PKY13_00325"
     CDS             complement(10263..11606)
                     /locus_tag="PKY13_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT78393.1"
                     /translation="MNHDERVRSVVSAVRAAAASAGPAHIDKGGVHHVVPLPGDRRFS
                     SRSIDTSALTNILEIDETARTCTAEPGVTFETLVRATLPLGLAPTVVPELRGITLGGA
                     VAGCSVESMSFRYGGFHDSCIEYEVVGGDGTVHTLSPTRSPELFEHVHGSYGTLGILT
                     KLTFRLVPAGRYVAMTYAHYSTAVEFEAALAEACRLDVEHDHDLVDGIVHGPRHLTLC
                     LGRFTDDAAGAEPSSYVGEHIFYRSTEKRRTDLLTSEEYFFRYDAECHWLTATAPPLQ
                     WRWVRKAFGKWILGSTNLIGWSNKLAPILRRVNRRPDLVCDIFLPQNRFAEFWAWYCR
                     VFDFWPLWVVPYRPASVYPWLGPHVRAGFAEGDLFIDFAVYGKRNVERTRDYSVLLED
                     ETYDLGGIKTLIGRNHYTRERFWEIYDRPAYEAARAALDPNGLFPDLYDKLGRVD"
     gene            11665..11880
                     /locus_tag="PKY13_00330"
     CDS             11665..11880
                     /locus_tag="PKY13_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78394.1"
                     /translation="MADADVLDSDLLDFECPRCQVAVTEPYYGPCGSCRDLMRANVAG
                     LARDDLEAEAYVPKMNVTPNAVALKDD"
     gene            complement(11906..12481)
                     /locus_tag="PKY13_00335"
     CDS             complement(11906..12481)
                     /locus_tag="PKY13_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT78395.1"
                     /translation="MRLPAAERREQLLTVAVDVFASNGFHKTSMNEVAEAAGVTKPVL
                     YQHFASKRALYLELLAEIGAELRDTIAKATADASGPRQQIDLGFTAYFGFIADNVNAF
                     RVLFGDSTRRDEEFADEARRVEASIAELAAELINVEGLSWERRLLLGNGIVGLAEGAC
                     RYWVTNDVEVDAATLAAQVSQLAWAGLRGVG"
     gene            12577..13092
                     /locus_tag="PKY13_00340"
     CDS             12577..13092
                     /locus_tag="PKY13_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78396.1"
                     /translation="MHPRSRTRRPAAAAFAVPAFIVPALVALALVSTSCTSDSDADDA
                     ATATTVARTSDPSTSVVESTTGAPTVPPTTAAPILATFPSPAAAGTALFSVWTSGDRA
                     GAGALNLAPPAELDELFAASPLPDAKNRGCDDGNFDAASCFFGNGQGGVDVGLVPAPG
                     GWSIATIDPFG"
     gene            13129..14358
                     /locus_tag="PKY13_00345"
     CDS             13129..14358
                     /locus_tag="PKY13_00345"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOT78397.1"
                     /translation="MTIMTPLGTTAASAPAPAPASAERWARVGHAGHTDARTAVAMAY
                     GELDLPDRPELVMMYVSASLDPAAAAAAIADLAPGVSLVGCTSRGELSHTGPAQDSVV
                     LVALGGAGVRCRTAIAPIVDGDARTAAATAARCADELDPLASTVMVVFSDGSDIDQDD
                     VIRGAYDTVGAAIPLVGGGACGAHLQFDPTLFRQDETGIVATSGVIVVAAITSTGPIG
                     IGVGHGFDPDGEPEVVTASGRDVVTTLGERPALDAYLRHHDAPADLFDTPDGFADFAR
                     ARPLGVARRTRVEPRAVVGADQTARTLTMGATVPPGGLVWLMHGDLESALGAIDVAAD
                     DCLDALGGRAPLAVVAFDCAARRTVLGGDGLVAEVDRLAGRAGCPVAGLYTYGEIART
                     SGSTGFHNLTVVLLAFG"
     gene            14376..16655
                     /locus_tag="PKY13_00350"
     CDS             14376..16655
                     /locus_tag="PKY13_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOT78398.1"
                     /translation="MAQQLAEFTSVFDGEDDVRRISLALDRVAESFDAEFALVVLDGS
                     VRQALGLNDDDLRSALLAAPPGSSVVEEGGHGSIHVTVVPIPRSGTDDRLAVGRLSDA
                     LSAVEVTLLRSMARTLGVALELHEARAVERQLHDEIAAQSRSNARLVESLSQRQRVLD
                     HLFRIQRSISERAPIMTVLDSITASAAELLPSTIIGLRLVESSGDTAALVAVVGEDGI
                     LQRTNERVPIGEGIAGRSIAENRFLVADDYPTSPDSHERWSALGVTTAMATPVRRNGV
                     AVGTLVAMSWEPGRRFDEEEQHLLQSLAEHGSLAFNDASAIEVIQRSLERAVFDSHHD
                     QLTGVANRKQAIGRLEEWAATEAGTTVIFVDLDRFKSINDAYGHTTGDEILRLVASRL
                     SEVVRVDDLVARLSGDEFLVIARSPDDDIVDDLAARVAETLSFVAQVGFREIRVTASV
                     GCARHIPGETADDVVANADLAMYRAKESGGGQLVRFDGAMRDERNAELTLERELRFAI
                     EHDEFDVFFQPVVDLESGQLIGAEALVRWNHPTRGMLAPGHFVDAAEAVGLIGEIDTL
                     AMRRAVHHLEGWDAAGQLAPEFRISVNVSARRFGDQRLVDTVRSELEHRSLAPSRLWL
                     EITETAMMRDVEGSLCTMQALRKLGVHLSVDDFGTGWSSLAYLKQFPVEALKIDQSFV
                     AGLCENPDDHAIVEATIQLAAALGLGVIAEGVEEQAQADELRRLGCRSVQGYLYSRPL
                     PAATFEAQWLGQPVSMPVY"
     gene            complement(16652..16945)
                     /locus_tag="PKY13_00355"
     CDS             complement(16652..16945)
                     /locus_tag="PKY13_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78399.1"
                     /translation="MRTNDKLDDVTASDDPVLIRRAQVARWSAMAKRAGYLTILVAMI
                     VFVIGFATEFGPAVTGIIVGCLVASAVLLIPALIFGYGVKAAEREDAGEPFRY"
     gene            complement(16932..17642)
                     /locus_tag="PKY13_00360"
     CDS             complement(16932..17642)
                     /locus_tag="PKY13_00360"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HOT78400.1"
                     /translation="MAALLTLLAIVAGGVVGLTHGGRIDRILEYRPQLWPALAGGLFL
                     QLVIRVAGLDGSLAVVLEIISALALLAFAVSNVRLGGMVLVVAGLSLNLIPTVINWGM
                     PTSRDALVTADIIKASDRGTVKLEGARHVATDDDTLTWLGETIALPTGQVISFGDTLL
                     HFGYLFVTASLLRGRVLRNDGGGDYRRRIAPLGQGPARRHGPGLHPSRLDPRAMAAAR
                     RARGDSGAGPEEAFDEDE"
     gene            17705..18484
                     /locus_tag="PKY13_00365"
     CDS             17705..18484
                     /locus_tag="PKY13_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT78401.1"
                     /translation="MSDELLASSADGVLTLTINRPERRNALSWDVITGLRAAVAAAKD
                     DATVRAVVITGAGEKAFCAGADLGGMADNASYAEVHDARGELARLFLDLYGLGKPTIA
                     KVRGFCLAGGFGLALACDLVIASDDATFATPEINVGLWPYMITVPLTRSMPPKKALEL
                     MMTGRRIDAAEADALGLLTRVVSAADLDGAVAETAALLASKSPSVMKMGRDSFYSVWG
                     RNAADSLAQLHPLLTLNTSLEDAAEGITAFLEKRDPVWTGR"
     gene            18513..20090
                     /locus_tag="PKY13_00370"
     CDS             18513..20090
                     /locus_tag="PKY13_00370"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT78402.1"
                     /translation="MTEVDERYNWGPLVADLAQRKETALAMGGPERVERQRSLGKLPV
                     RERLDLFLDPGTFVEYGMLADHMDPGLASKGSFAADGCITGIGNVDGRPVAVVAYDFT
                     IMAGSMGEVNEQKVTRLRELVLRQRIPVVWLLDSAGARVGSGTGSTFAGAGALFREQV
                     TLSGVVPQVAALMGHCAAGTAYIPALTDFIVMVKGISSMALAGRHLVKAATGEDVTEE
                     EMGGSDVHTKVSGVADLAVDDDVACLDAVKKYLSFLPSNNTTPAPRKETSDPADRRAE
                     EIYEIVPTAPRRAYDMVKVVKAIVDDGDFFEIKAAWARNIVCGFARLDGESVGIVASQ
                     PKVLGGALDVNAADKSARFVWLCDAFSIPLVFLHDVPGFVVGTVVEKQGIIRHGAKML
                     FAISEATVPKISVILRKSYGAGYFVMNGRAYEADYLVAWPTAEIAVMGPDGVVNIAFR
                     KQLEEAPEGPERDKLRIELAEMIRANIDPWVAAGHALVDDVIDPADTRAAIIAGLNAN
                     RNKQVFRPWRKHGVMPV"
     gene            20157..20369
                     /locus_tag="PKY13_00375"
     CDS             20157..20369
                     /locus_tag="PKY13_00375"
                     /inference="COORDINATES: protein motif:HMM:NF004547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HOT78403.1"
                     /translation="MDVTAEITANVWQVRTEVGATVSEGDELVILESMKMEIPVEAPI
                     SGTVTSIAVKPDDQVTEGDLVLTIEP"
     gene            20366..21130
                     /locus_tag="PKY13_00380"
     CDS             20366..21130
                     /locus_tag="PKY13_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT78404.1"
                     /translation="MTGSVTAGSEAVEPIRCEVTDRIAVVTIDRTARRNALNLDALHR
                     LDDAVASAVAGGAAAVVFRGEGGHFCAGADLKELEDQSFDTRLRGVLERIATAPVPTF
                     AAISGACMGLGMQLALACDIRVATDDAYFGIPAGKLGLVVNHWTLQRLALNLGFGAAR
                     LMVLTAETFTSDDAWRLGFTQVRGDLDAAVALARRSADLAPLSLQGSKIGLNLVERAL
                     DEPEFNAAFERAWNSADLAEGRLAFAERRSPVFKGV"
     gene            21263..22690
                     /locus_tag="PKY13_00385"
     CDS             21263..22690
                     /locus_tag="PKY13_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78405.1"
                     /translation="MASARFLKRSFATLLTVLVAGCSAPSSLGNPPRMPVAKPVGAPT
                     TSSTAPATPSSPTSPSTTAANPTQANCAGSYERFFACDASWNRPASHFGDATGRLASY
                     GTRFWKYGGDFVGGNWKPGDINMHFRDYSVPIYDLRTAINGRYRAYQAIEYQKISMFS
                     PGTRNVTIGQIIPYNPAWIPGTGTDRLMIAVNPDTGETWEFGGVNLPLGAFGVNQCSG
                     FLGPNALAPVPFDPGNPTHICFYGMAHYYNLYAATDGTTIAGRGMGINKMALLTRASE
                     VARAVANDTSIGHALEMTLPATMPGEPICPVNDGRFTDPGAGVSCGTFVSPATFVERA
                     QGTRDWCGPGQRSIHDPALPSTWLRTVPEGLRIRLEMTDADIVAWLDDRARTNPAYGR
                     GQPLRATAQAFARTFVEYGAIVAETGCAGIGIETEGLQGPAKSTWAELGIVDPSGTNP
                     YPAGDLLYGLITEARIRVVQPPEPR"
     gene            complement(22706..23407)
                     /locus_tag="PKY13_00390"
     CDS             complement(22706..23407)
                     /locus_tag="PKY13_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78406.1"
                     /translation="MLVIAMVMLTGACEPQPVGPPSVTSTRPRCPFDDGIVIYGGDSL
                     VTQWPRWVPLPPELTPYNTARGGSAYTGNLTPDPDYGTIGSRILDDLDDCGNDVGAVV
                     ISGGHVDLSYGRSAEAVIESIGELDEALHARGVEAVFLEITPVSNAADWYTVHQVARQ
                     TINAWMATPGNLHGAVVDCSPVLETTPASDVLAPRYWNFIDAFGTIDLVHPNDAAYEA
                     IGTCVQPAILAAVER"
     gene            complement(23599..24159)
                     /locus_tag="PKY13_00395"
     CDS             complement(23599..24159)
                     /locus_tag="PKY13_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HOT78407.1"
                     /translation="MILAGGSPARRRLPALACAAVAAVLLTGCSGSKDADDEGKAPAA
                     TTTTTSTPLVGTLTPTEAVIAGQCLDDVPDPAQQTYAVMVIPCEDPHTYEVYAQAKLD
                     LGATPTQNGAPYPGALAVANGAESQCVGAFPQFMGVKWEESEYDIQSWWPSEQSWATK
                     KDRTILCAVYRVTGGKTKGTVRGSGQ"
     gene            complement(24180..25214)
                     /locus_tag="PKY13_00400"
     CDS             complement(24180..25214)
                     /locus_tag="PKY13_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HOT78408.1"
                     /translation="MINRILGGLGRTLIFSGIVLLLFVGYQLWGTGLDESGHQEDLTR
                     SLGHSLGVDTAKTDAEEEDAAAIDEKITAKLSSIDPTKAPPAEIPKEGDPEGKIEIPA
                     IGLRSKTFVEGVAKADLRKGPGHYPSTPLPGNPGNAAIAGHRTTYGAPFNRIDELKPG
                     DQIITYTLQGKFVYEVLESPVERRAGDEGGTWGPGWFAVKPNDVSVLAPTDDNRLTLT
                     ACHPKYSAKLRIIVQAKLVAEPAEAPTTTVPTTDPATGADGTDDAQTVTPPLETTEDL
                     IAGDPGELAPALLFGGIVIALWLGSMALAALFRRREMRWWPIYIVTTVATVAPLWFCF
                     VHMDRFLPSY"
     gene            25351..26043
                     /gene="ccmA"
                     /locus_tag="PKY13_00405"
     CDS             25351..26043
                     /gene="ccmA"
                     /locus_tag="PKY13_00405"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HOT78409.1"
                     /translation="MPLDPVIQLHGAVALLGQFPVLAGVDLEVGDGERVLLRGPNGAG
                     KTSLLRTCAGLIPVSSGSATVLGHDLRADRRSVRPLVGLMAHTTGLYDELTAADNVRF
                     WAEACRVDPADAAGAMDRVGLATRLRTVPVNRLSTGQRRRVSLAAMIVRRPRLWLLDE
                     PHAGLDTVGRDLVDRLIGDAAAAGATVLFASHDLERADAVADRTVSIVGGAVAEDTGP
                     RPADRTGADDVA"
     gene            26033..26701
                     /locus_tag="PKY13_00410"
     CDS             26033..26701
                     /locus_tag="PKY13_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOT78410.1"
                     /translation="MWHDVWLVCRKDLRIEARTRVTLNHVAPFVLTVVMLFAFALDAD
                     TTLLRRATGGLFWVTVLFAANAIAQRSASVDRPDGLPDALRLSNLSPAGMFLGKTLSL
                     FLQLTATELVLAVAMVLMYDVRLRGIALILASIPLGTLAIAAVGSVYGPLAAGLQGRE
                     SILPLLMLPALAPVLLAATNAFGVAFGTTVGPGWRWVVMLGMLGAIYVAAGTATSAAL
                     LEET"
     gene            26739..27542
                     /gene="ccsA"
                     /locus_tag="PKY13_00415"
     CDS             26739..27542
                     /gene="ccsA"
                     /locus_tag="PKY13_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOT78411.1"
                     /translation="MTDQTSAAPAGTADTAKPVAMSTSSRGTKALGLLTLVSAVFTIL
                     YSFWFSKADVELGNTVRILYVHVPTVSIAYLCMLVNAGASAFYLWKRTAFPDLLAAAT
                     GEIGVLLLGLTILAGMMWGKPTWGTFWEWGDPRLTSTLLLFLMYLGYVTVRNIPAPRS
                     ARNTRSAVIGIISALLIYPVKMSTEWWGSLHQGRTISAPGASKIHGAQEFALYSGFLT
                     FMLFAAWLTIHRFRVGWLQDQADATELDAVIADRRAEAGAEQFGLGEVH"
     gene            27542..27700
                     /locus_tag="PKY13_00420"
     CDS             27542..27700
                     /locus_tag="PKY13_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78412.1"
                     /translation="MIAQQIQNGEAWTYVGITWGATAVVLGGYVLLILRRGKALSKRV
                     PEDKRRWM"
     gene            27691..28245
                     /locus_tag="PKY13_00425"
     CDS             27691..28245
                     /locus_tag="PKY13_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOT78413.1"
                     /translation="MDVSSIDDPESVDAGPLDLTPRSAPTASSGPAVRRRKIVPIVLG
                     VVVVAAIAVLLITQLAGAATYFYNVDDAVAKKADIGDKRIRLQGNVIKGSEVGRTQDS
                     VQGYVIAYNGVQVEVEQSGDVPDLFGPSIPVVIEGRFKGDLFLSDRVLVKHDETYDED
                     NSDRIKDAERDAEKNVSSTTIPTP"
     gene            28254..30284
                     /locus_tag="PKY13_00430"
     CDS             28254..30284
                     /locus_tag="PKY13_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HOT78414.1"
                     /translation="MSVALGNAAITLGLVACIIGVGTLVLGIRRGDEKLLIWSRPVST
                     VALVAAVAAVGVMQIALLTNDTSVKYVADEGSKRTPFPFDIATMWSALEGSILLWGLL
                     LTIFTAAVAAKFRRRLTDPLVAWAMVVMLTVTGFFFTLMLAVAPVFRSFDAPVGFDGP
                     GPNPLLQNHILVAFHPPLLYLGYVGFTVPFAFAIAALVTGRVGEGWLAETRRWTLFAW
                     GFLTAGIVLGAWWSYEVSGWGGYWAWDPVENASILPWLTGTAYIHSVMVQERRGMLRV
                     WNLTLVVATFSLTILGTFLARSGVVESVHAFSSGSIGPTLLGFFAFIVVASIGLIGWR
                     GDRLRSPGAIDSPLSREGAFLANNVLLGAFAFVILLGTVFPLIVEAVNDDRMSVGAPF
                     FDRMAIPIGLVLLFLMAVAPVLPWRSASTDLLSKRLWWPLWASVVALGFAVAVGARGV
                     TSLLGFWLAGFAAGSVARQLTLATRRQGWRGLVGRANGGMVVHLGVLIISVALVASQS
                     YTKQGEFTLERGETVRVGGHTFEYLSSSVVELSNRRELRSKIRIDGEDVFEPSMNQYL
                     STGMSIARPSVKVTPTTDLYLSITRPASADRPDIRVRIVVEPLIVWLWIGGFVMVAGT
                     VLSAFPGRGRRNPLDPVSSPIGHDRDPEPVAADEPEPEPDADVDTEPEGVPA"
     gene            30281..30844
                     /locus_tag="PKY13_00435"
     CDS             30281..30844
                     /locus_tag="PKY13_00435"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HOT78415.1"
                     /translation="MKHRSALIAGVVGVVAVALIVLFLTADIDGNPLDTDTNQLVGKP
                     APAIDATDTEGRPFAVSDYRGRWLLVNFFATWCAPCIKEHPELVAFSEAHSRLGDASV
                     VSVAYNDEPDLVNEFFERNGGDWAVIADDKSDFSIDYAVVGLPESYLVDPDGIIVHKF
                     IGGITKAEVEAVMADQGPATTTTVAPS"
     gene            30844..31341
                     /locus_tag="PKY13_00440"
     CDS             30844..31341
                     /locus_tag="PKY13_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOT78416.1"
                     /translation="MNGPASRRTRLLGWAGALVAVVVLVGLGARTTPTSGTSEDRVYA
                     LAGQMKCLQCLGESVAGSQADIAVKMRTEIRAQMRKGRTDDEILSSFADTYGQRVLLN
                     PSGDGVASLVWVIPVVIAGLGTVGLALAFARWRRQRNDAGTTEVTDEDRALVDAALAD
                     GANGG"
     gene            31418..32602
                     /locus_tag="PKY13_00445"
     CDS             31418..32602
                     /locus_tag="PKY13_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78417.1"
                     /translation="MNLDELARRTEERDFLLASIADLDREHAAGDVDPDDYDELRRGY
                     VARAAEAIRAVDADEATLRSQRDARPPRRGRALIWVGGVAVFTVVAGVLLANALGSRG
                     QGAGLTGTGSMPGDQASTCRSLSMSKPAEGITCYDKVLEDQPDDVDALTYQGWAKVRS
                     GDVEGGSAQFDRVIELDPGFADVHVFKASVLKNAGDFVGAKAELDRVYALNPSPIVTS
                     TLEQMGLDTEIAVGLLPADVQACWKDEKAALDATVAVTSKADAEIDRSAYAGALADVA
                     LSAKCLDTVLAGRPTDADALVLRSLAVGVLGLIDGSTYDAAEADATAALAARDGDPAA
                     LLLRALWRDNLGNVEGAAADLDALGDQRVSPLVTAYVEVAPVRAQVEADRAAARSTTT
                     TR"
     gene            32643..33851
                     /locus_tag="PKY13_00450"
     CDS             32643..33851
                     /locus_tag="PKY13_00450"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOT78418.1"
                     /translation="MKILVVGAGGVGAAFAAIASRRSFFEQLVLADIDVSRAQRTVDR
                     LGDPRVTAISLDASDATSVAAAIGAHGSTIVLNAADPRFVVPIFDGCFAAGATYLDMA
                     MSLSHPHPSDPYAQVGEKLGDYQFDRSSQWEERGLLAVGGMGVEPGLADVFARYAADH
                     LFSAIDEVGVRDGSNMVVKGYDFAPTFSIWTTIEECLNPPVIWEKERGWFTTEPFSEP
                     EVFVFPEGIGPVECVNVEHEEVLLVPRWVDCNRVTFKYGLGDEFIDVLRTLHKLGLDS
                     TDPVSVRGQQVSPRDVVAACLPNPADLTDEIEGKTCAGTWVTGTGTDGNPLEVYLYHV
                     ADNAETLARDGAQAVVWQTALFPVVAMELIAAGVWSGAGVKGPEAFDAVPFLDMVKDD
                     YESPWGLEIR"
     gene            complement(33899..34528)
                     /locus_tag="PKY13_00455"
     CDS             complement(33899..34528)
                     /locus_tag="PKY13_00455"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOT78419.1"
                     /translation="MTQIAIAMFPGMTMLDAIGPYQVLTWLPGAEVVLVGAERGRLTD
                     DKGLLHVEIEHTYDEVASPDVIVVPGGVVTRSMAAAGNPLVEWVRSAHPATTWTTSVC
                     TGALILGAAGVLDGLDATTHWIAYEQLAEFGAHPTEERVVVRGKVITGAGVSAGIDMA
                     LRLVIELAGIEVAQAIQLGIEYDPQPPVDAGSPSKAPDEIVELVRAMNA"
     gene            complement(34525..35526)
                     /locus_tag="PKY13_00460"
     CDS             complement(34525..35526)
                     /locus_tag="PKY13_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HOT78420.1"
                     /translation="MPHHRAPSTRRVAVVAFAKAHSLDICGPLEVFSAADNVLRSAER
                     PGYDIGVATIDGHPVRCSSGIELGATWSLGELLADPPDTLLVAGGRGSHETSADPEFL
                     TRFRGVAEVTRRVGSVCTGAFVLAATGLLDGKRATTHWASADRLAAKHPEVDVQCDLI
                     YTCDGETWTSAGVSAGMDLALAMVAADHDDEIARQVAQWLVLYLRRSGGQSQFSTPVS
                     SGTARRDEIRIVQDRIAADPSIDCSVETLASVASMSPRHFARLFREQVGTTPARYVES
                     CRVDLARTLLETTDLTVAEVAHRCGVGDASTLHRLFERRLATTPASYRSHFKKDHTT"
     gene            complement(35666..36334)
                     /locus_tag="PKY13_00465"
     CDS             complement(35666..36334)
                     /locus_tag="PKY13_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78421.1"
                     /translation="MKTTVRLAAVASLTAASLGLVAVAPAGASTSGPVATYTKAKCNQ
                     AIDERLFILDISEQRIEKVKRLTADQKAAQIAGIDGVQANLVNVNRPAVNNAKGKAGI
                     KAACSAIYADNRVYAVVIPQLFASVRIDEFGNAFDKFDPLIAEKKAAGADTSAIEALQ
                     VSAKAHVDAAAALVSGITPDSFNADPAGVKAKFAQATDELQAALGDVLTGIVAYRDLT
                     VPVP"
     gene            36423..37742
                     /locus_tag="PKY13_00470"
     CDS             36423..37742
                     /locus_tag="PKY13_00470"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HOT78422.1"
                     /translation="MTVRIGGGQGFYGDGHLPVAALLADGIDYLVCEALAELTLAILQ
                     KDRQRDESLGYTRDLPLYVAAAIGDILGGRTKLITNAGGINPIGAGRAVVGALAAHGV
                     TGVKVATIVGDDIRPHAEPLGLAADTLFANAYLGARPIVEALERGADIVIGGRIADAS
                     LFLAPLIHEFGWSPDDWDRLAAGVAGGHLLECSGQVTGGNYSGDWWNGLDPASIGFPI
                     GEFEPDGSFVVTKPAGSSGRVTFDTVREQLLYEVQDPTAYLNPDVTVDLASAHLDDLG
                     NDRVRVSGVRGAAAPATYKGLECTPGGWSNELRATYGWPDAEAKARAAVAYLRGRAAA
                     IGMDVKEWHEEYFGVDAFGGPTVPAAPAGWEPPEVIARLAWRCDTAEEAGRLGREAGL
                     LGLSGPPMIAGAGRARDGKPSQLLSLRALAVDRALVDAQVRVEITEV"
     gene            complement(37750..38211)
                     /locus_tag="PKY13_00475"
     CDS             complement(37750..38211)
                     /locus_tag="PKY13_00475"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HOT78423.1"
                     /translation="MLYLVRHGSAGVRDDADPADNERHLDAVGLRQAVQLAERLSGLE
                     ITAVVASPAARCVETVELLASSLGLAVDIDARLFEGTDIEESWALVEALVEHDEVAVL
                     CSHGDVIPELVRRAKGRGMEVPGKAGCSKGSLWSLEWDGERFATGTYEPMR"
     gene            38352..39629
                     /locus_tag="PKY13_00480"
     CDS             38352..39629
                     /locus_tag="PKY13_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78424.1"
                     /translation="MRGTRRTVAATALAVVAVLASGCPVEPTGPTDPTGPVWTPHHAG
                     GPAVGGATPWAIAIGTTEDWYAQLDVGFPLTAPPTVSVFPRTGPDGHSLGAPQTFTAN
                     MIGLGAGVLSEHLLLTGGDPASGSTALQFLAETGGTWSIAGSFSLESGETIRGMTDDA
                     LVTRAVGASGAEVRVYTLTRTTGSVTATLTQTLRPPAEWGPGYGTYWGSVVTLDGGLL
                     AVHAHNDSIGVPDRVGVYQSSAGSFGLVATLERPEFGAFGIRLAIDDQPAVDRLVIGG
                     YPTGSAEVVFAEYTSTAGGTWAAVSTIEPPAAVPGAAVGQTFGQGVALDGDVLVVTAV
                     DEPMATPGPGGAVRVEYRPLVYRLGAGGWEYEATLSVLSSPVDTDGLNRGLMSMVVAD
                     HHIVGTNGVSLPGGCSYCFGLRFEAWRWDRTPA"
     gene            39658..40029
                     /locus_tag="PKY13_00485"
     CDS             39658..40029
                     /locus_tag="PKY13_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78425.1"
                     /translation="MARLQLRDLCGVRSGDKGDISDLSLFADDEAAYEVIRSEVTAER
                     VAAHFGDLVHGAVVRHEVPNVWALKFVMDHALGGGGPQSLRADNLGKTLGGALLRLEI
                     DVPDDVAAAAPRNGRRAPLPN"
     gene            40082..40471
                     /locus_tag="PKY13_00490"
     CDS             40082..40471
                     /locus_tag="PKY13_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78426.1"
                     /translation="MGAHAVVWTVIALALGFVDFLDSWKVVLLGAWIGVAHAVLQWAT
                     TSVIVVDGVLVSRNFGWVQRVPIDEIELVQRVRHRRWSGVMLQLRSGQEVTLAAPLSS
                     VLAPNPHFEEDLNRLCASIAFGEVNGE"
     gene            complement(40458..41174)
                     /locus_tag="PKY13_00495"
     CDS             complement(40458..41174)
                     /locus_tag="PKY13_00495"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT78427.1"
                     /translation="MTTDETPRTDRFGNPIGPPIGDWTPPPAPDGRPLIGRTCRVERY
                     VPEVHSATLFEEYRADDGRSFTYLPFERPESVDELTRIVEAVGRPDDQLPYAIVVDDR
                     AVGMASYLRIAPPIGSIEVGAICFSPALARTTAATEAMFLMADAAFAAGYRRYEWKCD
                     SLNGPSNAAADRLGFTYEGTFRNAMVYKGRSRDTNWLSITDDEWPRRRAALVAWLDPT
                     NFDATGAQRTSLRREPVTRR"
     gene            complement(41196..42332)
                     /locus_tag="PKY13_00500"
     CDS             complement(41196..42332)
                     /locus_tag="PKY13_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOT78428.1"
                     /translation="MARAYLDHASTAPLRPVAADAMAEVLRQSSRGELGDPGRSHHEG
                     LVARHGVEHARERVARALGARPREVVFCSGATEAIAMAAHGAARRGPDPSAAHTVVSA
                     VEHSAVRAWADRGPATVVGVDRGGRVDPDAVIAAIGPDTAVVHLQWANHEVGTVQPVA
                     TVAAECRARGVLLHVDAAQAIGRVPIAFDESGIDLMSVTAHKFGGPTGIGALLVRRGV
                     RLDPLLPGADQERARRAGLENLTGMAGFAAALDEATLELDAEAERTAALTQRVIGWAA
                     NEPGVDVLGDPVHRMPHLVCLAIAGIEPQPILIGLDRAGVAVHSGNSCSSEALEPSPV
                     LAAMGVDAHHSLRVSVGWSTTDDDIDRLLTELPRVLGELRGLRG"
     gene            complement(42339..43073)
                     /locus_tag="PKY13_00505"
     CDS             complement(42339..43073)
                     /locus_tag="PKY13_00505"
                     /inference="COORDINATES: protein motif:HMM:NF037269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT78429.1"
                     /translation="MATAPTPDITNRLNPTDFASAVSAITSAFGDPTRREIFLFAHES
                     ADGVTASEVAQRFELHANVARHHLDKLAAGGYLEVATVRASGAGRPSKRYRVLSDHMP
                     FEMPVRHDDILINLLGRALAELGEERATALAEQVGVDYGRAMADAMGDTTAGQRSVRT
                     ALHAVADALSAHGFASRAEQHGDQLRIVSEHCPFGEAVIEHPVICAVDRGMVSGMLGA
                     LYGETPVDLSSSLPKGDARCITDIDS"
     gene            43197..44336
                     /locus_tag="PKY13_00510"
     CDS             43197..44336
                     /locus_tag="PKY13_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOT78430.1"
                     /translation="MSLNETAVIEALRPVQDPELHRSIVDLGMVRDVVVDGTKVALTV
                     VLTIPGCPLKGEITGRVNEALGTIGVTDVDLAWGAMNDEERAALRQLLHGDPASTAGS
                     QQAHGHAEGRAIPFADPLSRTRVLLIASGKGGVGKSSVTTNLAVALAQRGKRVGIVDA
                     DVWGFSIPKMLGVDRAPVLLDQMIVPPEVNGVSCISMGFFAEEDQAVIWRGPMLHKAV
                     EQFLTDVFWDEPDYLLVDLPPGTGDISISLAQFLPRAEVYVVTTPQPAAQRVAQRAAS
                     MAKKVNLPLRGIIENMSWFTGDDGKRYELFGEGGGQRLADDTGVPLLGKVPLLPALRE
                     GSDTGHPVVVAFPDTEAAQAFRAIAETIDVEMAPTRRFNRELKIG"
     gene            44491..44724
                     /locus_tag="PKY13_00515"
     CDS             44491..44724
                     /locus_tag="PKY13_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HOT78431.1"
                     /translation="MDVRIGITQSMRELEVDLGDEVVRETLKADIAAAMANDDGILWL
                     TDKKGREIAVPTAKISFIEIGSPDAPRNIGFSV"
BASE COUNT         6510 a        15353 c        15783 g         7078 t
ORIGIN      
        1 gcaccacgcc ggctcgcatc cggtcgccca gctcgtgcgc cgcggccaac aggtagggcc
       61 caccgccgga aagaccgacc accgcgaact cgtcggcgtc gaggtgatcg gcgacctggc
      121 ggaggtcggc ggcgaactcc acgatctgct cgtagacgtg cggcgtcgag tcacccgttc
      181 cgggtcgctc caccgtcacg atgcgcaacc cgcgctcgac cgcgagggtc ccggcgtcgt
      241 ggggcagctg cgagcgggcg cccggcgtgc cgtggaacca gaacacggtg tcgccctcgg
      301 ggtcaccgaa gatggcccag ccgagctcgc ggccatcgtg caacgtggtc gtcccgtcgc
      361 ggtgcgcttg cagtggtgca gtcatggacc gacgattttc cacccgtcgt cggcgcggtg
      421 tcgagtccgt ggggcacgtc ggggtgatcg acccagccgg aatcggatcg tggggtggtg
      481 gggaacacca ctaccgtacg ggccatgttg cgactcttcg acaccgccgc gggcgccgtt
      541 cgcccgttcg aaccccgagt tcccgggcag gtgtcgatgt acgtctgtgg tcccaccgtg
      601 tacggcccac cccacctggg tcacggtcgc ttctcgctcg tgttcgacgt gctccgtcgc
      661 tatttcgaat ggtcggggct cgaggtcacc tacgtctcga acatcaccga catcgacgac
      721 aagatcatcg agcgggccaa cagcgagggt cgtccgtggc aggagatcac cgagaagtgc
      781 gaggcggtct ggtaccgggc gatggacgcc atcggcgtga agcggcccac ccacgacccg
      841 cacgcaaccg cctacgtgac ccagatggtc gatctgatct cgcggctcat cgagaagggc
      901 gccgcctacg agacctccga cggggtgtac ttccggccgc ggcaggtggc cgactacgga
      961 ttgctcgccc ggcaggacat cgattccctg caggtgggtg cccgcgtcga cgtcgtcgac
     1021 gagaaggaga cgccgatcga cttcgcgctc tggaagaagg ccaagccggg tgagccgtcg
     1081 tggccgtcac catggggcga cggccgtccg gggtggcaca ccgagtgtgt ggtgatgtcg
     1141 ctcgacctcc tgggcgaggg cttcgacctc cacggcggtg gccaggacct ctgcttcccc
     1201 caccacgaga acgagcgggc ccaggcggtc gccgacggca aggacttcgc ccatcactgg
     1261 gtgcacaacg gcttcgtcga ggtcggcggc gagaagatgt ccaagtcgct gggcaacttc
     1321 acgaacctgc tcgatctcat cgatcgctac gacccccggg cctaccggct gttgtgcctc
     1381 caggcgcact accggtcgcc gatcgacgtg aacgagaaga actcggcgca ggcctcggcc
     1441 gcgctcgagc ggtacgacgc gctcgcccga cgcgtcaccg acctcggcca cggcgtggcg
     1501 cccgatcccg tgatcatcgg ccggttccgc gatcgtatgg acgacgatct caacaccggc
     1561 ggggcgttgg cgatcgtggc ggagaccgtc acccagatca accggtccct cgacatcgac
     1621 gacttcgccg gtgcggagcc gctggtcgcg gcgttccgat cgatgttgag cgccgtcggc
     1681 ctcacggtga acgacgaggt tgcggccgtc gccgacgagg ccgccgacct gtgccgccag
     1741 cgtgacgacg cccgcgctgc caaggagtgg ggtcgcgccg acgcgctccg tggtgaactc
     1801 gaggccatgg ggtacgtcgt cgaggacacc gcgaccggca cccaggtccg gcgggcctga
     1861 tccgatgcgg cgtctgacgg cggtggtcat ggcgctcacc gtcgttgcgc tcggttgctc
     1921 gggcgaggac gactcgggga gtgccggcgg cgcactgtcg tcgagtcgac gcgacggcac
     1981 gacgtcgaca tcgagcacga ccgtggcacc accggcgccg cggtccacgg tggtgatcgc
     2041 ccaccggggc gcgtcggcct acgcgcccga gcacacgttc gcgtcctacg acctggccct
     2101 cgagcagggc gccgactaca tcgagcagga cctgcagatg acggcggacg gccagctggt
     2161 cgtgctgcac gacccgatcc tcgaccgcac ggcacgcggc cctgccgacg cgtgcacggg
     2221 gccggtcgcg tcgaagacgc tcgcgcagct gtccgcgtgc gacgtgggct cctggttcaa
     2281 cgaggcccac cccgatcgtg ccgatccggc gttcgcccag ctccggatcc cgacgctgcg
     2341 cgaggtgctc gagcgctacg ccggccgcgc acggttctac atcgagacca agtcacccga
     2401 ggcccagccg ggcatggagc aggcgctgct cgacgtgctc gacgaggtcg gcccgccggc
     2461 ggacggcacc tccgccgccg gcgagcgatc cgtgatcatc cagtccttca gcgccgagag
     2521 cctgcggctc gtccactcgc tgcgtcccga gttgccactc gtcctgctcc tcgtgaccag
     2581 cggcgagccg ctggacccgg cgatcctcga cggggtcggc gactacgccg tgggggtcgg
     2641 accgtcgagc ggcaacgtcg acgaggcgct cgtcgacgca gcccactcgc agtgtctcga
     2701 cgttcacccg tacaccgtcg acgacccggc agagatgacc cggctgatcg ctgccggtgt
     2761 cgacggcctg ttcaccaacg ctcccgacgt gctgcggccg ttggtggccg ccctcccggg
     2821 cggtcgtgac gcctgcggct gaacgcccga gcggtcagcg gccggtcagc gcccggagtc
     2881 gctgtcggat tcccagacga tgacctcggc gtcgccgacg ccggtgacgt ccacggcccc
     2941 ggctgccgtc aggcgggcgg cgtcgccttc gacgagggtg tcgccgccga gcgagaccga
     3001 gccgcgggca acgaagaggt gcacgtaggg ggcatcgggc agggcgacgg tctcgccgtc
     3061 gccgagtcgg ccgacccaca tgacggcgcc gggctgatgg atggtgatcg ctccctcgtg
     3121 gccccggccc gaggcaacgg ggacgagacc gccggcgtcg aggcgctcgt tgacgtcgag
     3181 ctgttcgtac cccggccgga tccccgtttc ggcgggaacg acccacatct gcaccagatg
     3241 caccggcaca ctccgcgacg cgttcatctc ggagtgctgg atgccggttc cggccgacat
     3301 gcgctgggcg aggcccgggt acaggaggcc gacgttgccc tcggagtcac ggtgctcgag
     3361 ctggccgtcg aggacccagg tgacgatctc catgtcgcga tgggggtgag tgccgaagcc
     3421 gccacccggg gcgacccggt cgtcgttcga gacgatgagg aggccgtggc cgaggttcgt
     3481 cgggtcgtag tgcgacccga agctgaagct gtgccgaccg tcgagccaac ccagctcggt
     3541 gcgggggcgg gtgtcggccc ggcggatgtc gatggtggcg ctcgtcgctg cggcgccggt
     3601 ggtggtgttc gtgtcggtca tggcgacccc cttccgagat gattgcaact gcaactatac
     3661 cagggaggaa ggtaattgca agtgcaatcg aatgctgcat gctggatagg atgaggtcat
     3721 ggcgaccgag cgcgaacctc acccgctcca gctggaggca tggcgcagct tcctcgaggc
     3781 ccacgcccgc gtgaccgagc tgctcgatgc cgagttgcgg gcgcagcggg atcttccgct
     3841 gggctggtac gacgtgctcg tccagctctc ggaagcaggg ggccggcgcc gcatgagcga
     3901 gttggccgac gccgtgctgc tcagccgctc gaactgcacc cggctggtcg accggctgga
     3961 cgcggccggc ctcgtcgggc gcgaacccga tcccgccgac gcccgggtcc gctgggcggt
     4021 gctcaccgag gccggtcggg cccgcctgaa ggacgcgtcg agtgcgcatc tcgccggggt
     4081 cgagcgcgcg ttcacctcgt tcgtggacga cgacgcggcg aaggtgctgg tgtcggtgtt
     4141 ccacccgatc atcgacgcac tccgagccca gcgctgagcc gactcctggt cagctgaggc
     4201 ccgcttgctc agcgcggcat cgttggcagt gtgggcgacc ggagccacgc gtcgaagaac
     4261 gggacgaggt cctgtccggc gagccgggtg gcgagttcct ggaagtcctc ggtggtcgcc
     4321 gaggtgccgt cccgctcgga cgcccacgtg cgcaggatct cgaagaacac gtcgtcaccc
     4381 acggtcagtc ggagtgcgtg gagcacgatg cccgcgccgt cgtactcgtt gggggagaac
     4441 aggaccggcg cagcgggcga ggtcaccgga ccgaacgctt cgcgccaccg gggagcctgc
     4501 gccatggcat cgtcgacggc gtcgtcgatc ggcgtcgttc cgatgtggtc attccacagc
     4561 cactcgccgt aggtggcgaa gccctcgttg agccagatgt cggaccaacg cgccggggtg
     4621 acggcatcgc cgaaccactg gtgagcgagc tcgtgggcca tcaccgaact cgtcgaggtc
     4681 gggtcgaacg acgtgagcca ctccgacggg agcagggtga gcgtctgggt ctcgagcgcg
     4741 aactccgggc cggagtcggc gacgaggagc ccgtaggtgt cgaacggaaa gggcccgaac
     4801 accgactcga agaactcgag ctgcgcctcg gtgtcatcca ggacctcccg ttggtctgcg
     4861 gtgacggacc gcacgaccgc gtggcggatc ggcaggccgc ccggcccggt cgactcgacg
     4921 atgtcgtagt cgccgaccgc gatctggacg aggtacgtcg ccatcgggtc ggactggccg
     4981 aacgtccacg tggtgtggcc gtcgccggtc gcagccttcg atccgaggac cccgttggcg
     5041 acgacggtgt cacgttccgc ggccgtgacc gcgatcgtga acgtcgcctt gtcgctcgga
     5101 tggtcgttgc aggggaagaa ccccctcgct ccggtcggct cgccggcgac gtaggtgccc
     5161 gacggtgcgg acaaccaccc gatcgtgccc ggtgcgctcg ggtcctgcac cggggtcggg
     5221 gcgccgtggt actgcacgac ggtggtgagg tcggcccccg atgcgatggg ggaggttggc
     5281 gtgatcgtga gttccgaacc ctcgtgggtg aaggtcgccg cctcgtcgtc gacggtcacg
     5341 tcgtcaacgg tgaggccctc gaggtcgagg ttgaacgcac tcaggtcctg cgtggcggtc
     5401 acctcgatcg tggccgtccc ctcgatcgtc gtgcggtcgt cggcgacggt gatggcaagg
     5461 tcgtagttcg tcacgtcgta cccgccgttg ccggctccgg ggacgatcgc gtcgcccacg
     5521 tcggcgctgc cggccgtgcc cgtgggtgcc gccgtggtcg tcgtggcggc ggccgttgtg
     5581 gtcgtggtcg atcgatcgac acgggcggtg atccggcccg ggtcgtcgct gcacccgccg
     5641 atcagggtcg ttgcgagcgc aacggcgagg acgacggccg gccggcgacg atgcatcggg
     5701 agagcgtacc ggtgggttcg ttcggcggct cctcgaccgg tcggcggccg ctcccgaagc
     5761 ggtcggtgtg cgctcggcgt tcggtctggt gcgcgaggtt accggcgggt aacttcgcgg
     5821 gtggcggctc cgccgacttg ttctgcttac ttgctctgcg cccgaccatc gtgcgctcga
     5881 tcacgaggtg acctgatgac cgatttctcg ctctcgctca acgaggacca gctccagttg
     5941 aaggagtgga tcaacggctt cgcgaaggac gtcatgcgtc ccgccgccga agagtgggac
     6001 gagcgcgagg agttcccgtg gccggtcgtg caggaggccg ccaagatcgg cctctacggc
     6061 atggacttca tggcgcaggg catcgccgac cccaccgggc tcaccatggt cgtggcgatc
     6121 gaggagctgt tctggggtga cgccggcatc ggtctctccc tcatgggctc gggcctcgcc
     6181 gccgccgcca tcgccgccaa cggcactccc gagcagatgt tcgaatgggt gccgcagtgc
     6241 tacggcacgg ccgacaaggt ccagctcggt gccctgtgcg cgtccgaacc cgacgccggc
     6301 tctgatgtcg gtggctaccg cacccgcgcc acctacgacg aggccaagga cgagtggacc
     6361 ctcaacggca ccaaggcgtg gatcaccaac ggtggcatcg ccgacgtgca cgtcgtcgtg
     6421 gccgtggtcg agcccggcat cgggtcgcga ggccacgcga gcttcgtcgt gccgcccggc
     6481 acgatcggtc tctcgcaggg gcagaagtac aagaagcacg gcattcgtgc ctcccacacg
     6541 tccgaggtcg tgctcgagga cgtgaagatc cccggcagct gcctcctcgg cacgaaggag
     6601 cgcctcgacg ccaagatcgc gaaggcgaag gagaacgtct cgaccggcga gaagcagccg
     6661 gcgatggcca ccttcgaggc cactcgtccg acggtgggcg ccatggccgt cggcatcgcc
     6721 cgggcggcgt acgagtactc gctgcagtac gccaaggacc gtcaggcctt cggcgagccg
     6781 atcatcatga agcagggcgt cgccttcaag ctcgccgaca tggcgacgga gatcgacgcc
     6841 gcccgtctgc tcgtgtggcg cgctgcgtgg atgagcgccg ccaaggtgcc gttcaagcag
     6901 ggcgagggct cgatgtccaa gctcaaggcc tccgaggtcg ccgtgaaggc gaccgaggac
     6961 gccatccaga tcctcggcgg ctacggctac acccgtgagt acccggtgga gcgctggcac
     7021 cgtgactcca agatcatgac catcttcgag ggcacctcgg agatccagcg gctggtgatc
     7081 gcccgcgccg cgtcgggcct gcgcattccc tgacccgcac cggcgggtgc gccgtcgaac
     7141 tccgtgagac cgtccgaagg gctgggcaga gatgcccagc ccttcgtcgc gcacgggaaa
     7201 atgccggctc ggtgtcaggg gtgccttcta gggttcgcac cgtgccattg atccaagctg
     7261 aacaactgac gaagagcttc ggcgacacga aagcccttgc cggcctcgac ctcgaggtcg
     7321 agcagggcac cgtgctcggc gtgctcggcc ccaacggggc cggcaagacc actgcggttc
     7381 gcatcctcac caccctcctg cgccccgacg gcgggcgcgc caccatcgac ggcatcgacg
     7441 tcgcgaagga gcctcgccgc atccgctcgc gcatcgggct caccggccag tacgcggcgg
     7501 tcgacgagcg cctgaccgcc cgtgagaacc tcgagctcat cgggcgcttc ttccgcatga
     7561 gcggcggcga ctccaagcgt cgggcgcaag aactgctcga ccggttccgc ctcgatcagg
     7621 ccggcgatcg tgttgtcagc ggattctccg gcggtatgcg gcggcgcctc gacatctcga
     7681 tgagcctggt cgctcgaccc acggtgctgt tcctcgacga acccacaaca ggtctcgatc
     7741 cccggtcgcg caacgccatg tgggatctca tcgacgagct cacggccgag ggcatgacca
     7801 ccctgctcac cacccagtac ctcgaagagg ccgatcgcct ggcgaaagag atcgtggtga
     7861 tcgaccgcgg ccaggcgatc gccaagggca ccgcggacga gctcaaggat ctgctgggcg
     7921 gtgcccgggc cgaggttgcg ctcggtgagg gtgccgacca gggcgcggtc attgccggcc
     7981 tcggcggcct ggtcaacccc gacgccgagc cggcggtcgg tgccgggacg gtgacggtgc
     8041 cgttgcgtca aggcaccaca accccccagg ttgttcgggc actcgacgcc gcgggcgtgg
     8101 ccgtgcgtga cgtcactgtc tcccgtccca ccctcgacga cgtgttcctc accctcaccg
     8161 gtcatgcagc agaagaagtc gcagccgacg acgaaggaga ccagtcatga ccgcgacgtc
     8221 gcccaacgca acttcgccct cttccgcatc ctcactcgtc ggtcgcgccg gcgacacggt
     8281 tgacgctccg ggtggcgtcg tctgggcgct ccgcgacagt tggaacgagg caacccgcca
     8341 ccttcgagcg gtgccacgaa gtcctgagct gctgctgttc gccaccttgc agccgatcat
     8401 gttcgtgttg ctgttcaact acgtgttcgg cggctcggtc aatgtgggcg gcgactacac
     8461 acagttcctc atccccggtg tcttcgccca gacggtggtg ttcggctcgg cgttcaccgg
     8521 tatcggtatc gccgaagacc tgcagaaggg cttcatcgac cgccttcggt ccttgcccat
     8581 ctcccagggg gcggtgctga tcggccggac gatgtcggac atggtccgca acgtgctcac
     8641 gttcctggtc atgttggggg tgtcgttcct gattggcttc cgcttcgagg gtggcctcgc
     8701 gaagggactc ctggccaccg cgttgctgct ggggttcagc tactccttca gctggatcca
     8761 ggcgctgatc ggcttgtcgg tgaagtccgt cgaggcggcg aactcggccg ggttcatctg
     8821 gatgttccca ttgaccttcc tgtcatccgc cttcgtgaag ccggagaaca tgacctcggc
     8881 gtggctacga aaggtcgccg agtggaaccc gttcacgatc gtgaccaacg cggcccgtgc
     8941 gctctacggc ggcacgcccg tgggcgacac ggtgtggcag tcgatcgtgt tcgccatcgc
     9001 gatcacggtg gtcttctcgt tcctggcggt ccgcaagttc aagagcgccg cctcgcacta
     9061 ggcagacccg ttctgccgac ggtgccggcc ccggccccgg ccccggcccc ggccccggcc
     9121 ccggccccgg ccccggcccc ccggcccact gatcgaactc ccgacgggga acccgcaccg
     9181 agcgggttcc ccgtcgaggt ggtcggtcgc ttcccctgca tgtccgtctc gttccaggag
     9241 acgaagcatc gtggacttcg gtcccattca cgagcgagcg tcaacccagc gcgcgctcat
     9301 cacgttcgac caactgctcg acgccggagt cagtcgacat caggtcgatc ggctgagcgc
     9361 ccagggcttc ctcgtgccga tccaccgcgg ggtgtttcgc ctcccgggca gcccgctcag
     9421 ctgggagcag cgtgtcgtcg cagccgctct cgccacggct cccgactcat ggatctccgg
     9481 accgcgcgcg ttggcatggt ggggcgtgcg cgacggaccc tcggttgagg tgctcacgac
     9541 ctcggcccgg cgagtccggc tcgagggcgt gcgggcccat cagaccatcg atctccctgg
     9601 ggtcgaccgc cgccggcatc gcgacgttcc cgtcacctcc atcgagcgat cgcttttcga
     9661 ctcgggtcgg tcactgacac accagcaggt cggggcggca ctcgatcacg ccgttcgcga
     9721 cgggctcacg acctacgagc gccaccaaca gcgagtcgag gagctggggc gccgaggacg
     9781 tgccggatcg gcgacagcgc aacgcgtgct gacggcccgg ggatacggcg acggtttcgg
     9841 gttcgagaag gcgatgaggg ggttgctgcg cgaggtcggg ctcccggcac cggtccgcga
     9901 gctcaaggtg catgtcgacg ggttcaggta ccgcgtggac ttcgcctacc cggattcgat
     9961 gctcggcatc gaatgtgact ccggtgagtg gcacggactc ttccaccagg tcgagtgcga
    10021 cctcgagcgg cagaatcgca tccagaagga ggggctgctc ctcatgcggt acacggtcac
    10081 gagactgcgc gacgagcgag atgcggtcgg caccgagatc gtcgaggcga tcgctcgccg
    10141 cgctggctcc acgcccgagc gcccatgggt ctttcccgac gtgaaacccg cctgacgcgg
    10201 gttccacgtc ggaacttcgg gacgggaggg cacgggcggg cggtgagtgg gggagcaccc
    10261 ggtcagtcga cgcggcccag cttgtcgtag aggtccggga agagtccgtt gggatcgagg
    10321 gcagcgcggg ccgcctcgta cgcgggacgg tcgtagatct cccagaagcg ttcgcgggtg
    10381 tagtgattgc gaccgatgag ggtcttgatg cccccgaggt cgtaggtctc gtcctcgagg
    10441 agcacggagt agtcgcgggt gcgctcgacg ttgcgcttgc cgtacacggc gaagtcgatg
    10501 aagaggtcgc cctcggcgaa gccggcgcgg acgtgcggcc cgagccacgg gtacaccgac
    10561 gcgggccggt agggcacgac ccacagcggc cagaagtcga agacccggca gtaccaggcc
    10621 cagaactcgg cgaatcggtt ctgcggcagg aagatgtcgc agacgaggtc gggacggcgg
    10681 ttcacccgcc gcaggatggg cgccaacttg ttcgaccaac cgatgagatt ggtcgacccc
    10741 aggatccact tgccgaaggc cttgcgcacc caccgccact gcaatggcgg cgccgtcgcg
    10801 gtgagccagt ggcactcggc gtcgtagcgg aagaagtact cctcggacgt gaggaggtcg
    10861 gtacgccgct tctcggtgga ccggtagaag atgtgctcgc cgacatagga cgacggctcc
    10921 gcgccggcgg cgtcgtcggt gaaccggccg aggcacaggg tgaggtggcg gggcccgtgc
    10981 acgatcccgt cgacgaggtc gtggtcgtgc tcgacgtcga gtcggcacgc ctcggccagg
    11041 gccgcctcga actcgacggc cgtcgagtag tgggcgtagg tcatggcgac gtacctcccc
    11101 gctggcacca ggcggaacgt gagcttggtg aggatgccga gcgtgccgta gctgccgtgg
    11161 acgtgttcga acagctcggg cgaccgggta ggggacagcg tgtgcacggt gccgtcgccg
    11221 ccgacgacct cgtactcgat gcacgagtcg tggaaccctc cgtagcggaa cgacatggac
    11281 tcgaccgaac agccggccac ggcgccgccg agcgtgatgc cacgcagctc gggcaccacg
    11341 gtcggtgcca gcccgagggg cagcgtggcc cgcacgaggg tctcgaaggt cacaccgggc
    11401 tcggcggtgc acgtgcgggc ggtctcgtcg atctccaaga tgttggtcag cgccgacgtg
    11461 tcgatcgacc gactcgagaa gcggcgatcg ccgggcaacg gcaccacgtg gtggacgcca
    11521 cccttgtcga tgtgtgcggg accggcggac gctgcggcgg cccggacggc ggagacgacg
    11581 ctcctgactc gttcgtcgtg attcaccgac acatggtcgc gcacatcgtc gcgcgcccgg
    11641 ctcccggcgc gcgatgctgg ggcgatggcc gacgccgacg ttctcgactc cgatctactc
    11701 gacttcgagt gcccgcggtg ccaggtcgcc gtgacggagc cctactacgg cccgtgcggg
    11761 agctgccgcg acctgatgcg ggccaatgtc gccggcctcg cccgtgacga cctcgaggcc
    11821 gaggcctacg tgccgaagat gaacgtcacc cccaacgccg tggcgttgaa ggacgactga
    11881 gcgtcgacag cgtcagccga cgccgtcagc cgacaccgcg gagacccgcc caggccaact
    11941 ggctgacctg cgccgccagg gtggcggcat cgacctcgac gtcgttggtg acccagtagc
    12001 ggcacgcgcc ctcggcgagc ccgacgatgc cgttgcccag cagcagccgg cgctcccagg
    12061 agagcccctc gacgttgatg agctccgccg cgagttcggc gatggacgcc tcgacgcggc
    12121 gggcctcgtc ggcgaactcc tcgtcgcggc gagtgctgtc gccgaagagc acccgaaacg
    12181 cgttgacgtt gtcggcgatg aacccgaagt aggcggtgaa gcccaggtcg atctgctggc
    12241 gcggcccgga ggcgtcggcg gttgccttgg cgatggtgtc gcgcagctcg gcgccgatct
    12301 ccgccaagag ctcgaggtag agcgctcgct tggacgcgaa gtgctggtag agcacgggct
    12361 tggtgacccc ggccgcctcg gccacctcgt tcatcgaggt cttgtggaag ccgttgctcg
    12421 cgaacacgtc gacggcgacg gtcaggagct gctcgcggcg ttcggcggct ggaaggcgca
    12481 tagagacctc gaagttacca gaggtgctgc gaggccccgc aggaccgacc tgccgtcagg
    12541 gtggggcggg gcggactcgt cggtaccgtt gccgtgatgc atccccgttc ccgaacccgg
    12601 cggccggcgg ccgcggcgtt cgctgtgccc gcgttcatcg tgccggcgtt ggtcgccctc
    12661 gcactcgtgt cgacctcgtg cacgtcggat tccgacgcgg acgacgcagc gacagccacc
    12721 acggtcgctc gcacgagcga tccgtcgacg tcggtggtcg agtcgaccac gggcgctccg
    12781 accgtcccgc cgaccaccgc cgcgccgatc ctggccacgt tcccgtcgcc cgcagcggcc
    12841 ggcaccgcct tgttctcggt gtggacctcg ggcgatcgtg ccggcgccgg cgcgctgaat
    12901 ctcgcgccgc cggcagaact cgacgagctc ttcgcggcct cgcccctgcc ggacgccaag
    12961 aaccgaggat gcgacgacgg caacttcgat gccgccagct gcttcttcgg caacggccag
    13021 ggaggggtcg acgtggggct cgtaccggcc ccaggtgggt ggtcgatcgc cacgatcgac
    13081 ccgttcggct agttcagaat cgaagcggat cggccgatat ctggagtgat gaccatcatg
    13141 acgccgttgg ggacgaccgc agcgtcagcg cccgcgcccg cgcccgcgtc ggctgagcgg
    13201 tgggcacgcg tcggtcacgc cggccacacc gacgcacgga ccgccgtggc gatggcgtac
    13261 ggcgagttgg acctgcccga tcgccccgag ctcgtgatga tgtacgtgtc ggcgtcgctc
    13321 gaccccgccg ctgcggcagc ggcgatcgcc gatctggcgc cgggcgtctc cctcgtcggg
    13381 tgcacctcgc ggggcgaact gtcgcacacg gggccggcgc aggactcggt ggtgctcgtc
    13441 gcgctcggcg gggccggcgt ccggtgccgc acggcgatcg ctcccatcgt cgacggggac
    13501 gcccggaccg cagccgcgac cgctgcccgc tgcgccgacg agctcgaccc gctcgcctcg
    13561 accgtgatgg tggtgttctc cgacggcagt gacatcgacc aggacgacgt gatccgcggt
    13621 gcgtacgaca ccgtcggagc cgccatccca ctcgtcggcg ggggagcgtg tggcgcccac
    13681 ctgcaattcg atccgacact gttccgccag gacgagaccg gcatcgtcgc gacgagcggg
    13741 gtgatcgtcg ttgcagcgat cacctcgacg ggcccgatcg gcatcggcgt cgggcacggc
    13801 ttcgatcccg acggcgagcc cgaggtcgtg acagcgagcg gccgcgacgt cgtcaccacc
    13861 ctcggcgagc ggccggcgct cgacgcctac ctgcgccacc acgacgctcc cgccgacctg
    13921 ttcgataccc cggacgggtt cgccgacttc gctcgggccc gcccgctcgg cgtcgctcgt
    13981 cgcacacgcg tcgagccccg ggccgtggtc ggtgccgacc agacggcccg caccctcacc
    14041 atgggcgcga ccgttccgcc agggggcctg gtctggttga tgcacggcga tctcgagtcg
    14101 gccctcggtg ccatcgacgt tgcggccgac gactgcctcg acgcgctcgg cggccgggcg
    14161 ccgctcgccg tcgtggcgtt cgattgcgcc gctcgccgca ccgtgctcgg cggggacggg
    14221 ctcgtcgctg aggtcgaccg tctggcgggg cgggcgggct gcccggtggc gggcctctac
    14281 acgtacggcg agatcgcacg cacgagtggg agcaccggct tccacaacct gaccgttgtg
    14341 ctgctggcgt tcggatgagc gtcgccgctg cgtggatggc gcagcaactc gccgagttca
    14401 cctcggtctt cgacggcgag gacgacgtcc gccgcatctc gctggccctc gaccgcgtcg
    14461 ccgagtcgtt cgatgccgag ttcgcgctgg tcgtgctcga cggctcggtg cgacaggccc
    14521 tcgggctcaa cgacgacgac ctgcgttcgg cgctgctcgc ggctccaccg ggttccagcg
    14581 tcgtggagga gggagggcac ggatcgatcc acgtcacggt cgtcccgatt ccccgctccg
    14641 gcaccgatga ccggttggcc gtcggccgtc tcagcgacgc gctgagcgct gtcgaggtga
    14701 cgctgctccg gtcgatggcg cgcaccctgg gcgttgcgct cgaactgcac gaggcccgcg
    14761 ccgtggagcg gcagctgcac gacgagatcg ccgcgcagtc gcgctcgaac gcccgactgg
    14821 tcgagagcct ctcgcaacgg cagcgggtcc tcgaccacct gttccggatt caacgatcga
    14881 tctccgaacg ggcgccgatc atgacggtgc tcgactcgat cacggcgtcg gccgccgagc
    14941 tgttgccgtc cacgatcatc ggcctgcgac tcgtcgagtc gtccggcgac accgccgcgc
    15001 tcgtcgccgt ggtcggcgag gacggcatcc tgcaacgcac caatgagcgg gtcccgatcg
    15061 gcgaggggat cgccgggcgg tcgatcgccg agaaccgttt cctggtcgcc gacgactacc
    15121 ccacgtcgcc cgactcccat gaacgctggt ctgcgctcgg tgtcacgacg gcgatggcga
    15181 cccccgtccg tcgcaacggc gtcgcggtcg ggacgctggt cgccatgtcg tgggaaccgg
    15241 gccggaggtt cgacgaggag gaacagcacc tcctccagtc gctggcggaa cacggatcgc
    15301 tcgccttcaa cgacgcgagc gcgatcgagg tgatccagcg gtcgctcgaa cgcgccgtgt
    15361 tcgactcgca ccacgaccag ctcaccggcg tcgccaaccg caagcaggcg atcgggcgtc
    15421 tcgaggaatg ggcggcgacc gaggccggca cgaccgtgat cttcgtcgac ctcgaccgct
    15481 tcaagtcgat caacgatgcc tacgggcaca cgaccggcga cgagatcctc cgtctggtcg
    15541 cgtcgcggtt gtcggaggtg gtccgcgtcg acgacctggt ggcgcgtctg agcggcgacg
    15601 agttcctcgt gatcgcccgg tcccccgacg acgacatcgt cgacgacctg gcggctcggg
    15661 tcgccgagac gctgagcttc gtcgcccagg tcgggttccg cgagatccgg gtcacggcga
    15721 gcgtcgggtg cgctcggcac atcccggggg agacggccga cgacgtggtg gcgaacgccg
    15781 acctcgcgat gtaccgcgcc aaggagtcgg gaggcggtca gctcgtccgc ttcgacgggg
    15841 cgatgcgcga cgaacgcaac gcggaactga ccctcgaacg cgagttgcgg ttcgcgatcg
    15901 agcacgacga gttcgacgtg ttcttccaac cggtcgtcga cctcgagagc ggccaactga
    15961 tcggggccga ggcgctggtg cggtggaacc acccgacgcg gggaatgctc gctcccggcc
    16021 acttcgtcga tgcagcggag gccgttggcc tgatcgggga gatcgacacg ctcgcaatgc
    16081 ggcgagcggt gcaccatctc gagggttggg atgccgccgg gcagctggcg ccggagttcc
    16141 gaatcagcgt caacgtgtcg gctcggcgct tcggcgacca acggctcgtc gacacggtgc
    16201 gatccgagct ggagcaccgg tcgctcgccc cgtcacgact gtggctcgag atcaccgaga
    16261 cggcgatgat gcgcgacgtc gaggggagtc tctgcacgat gcaggcgctg cggaagctgg
    16321 gcgtccacct gtcggtggac gacttcggta cgggatggtc gtcgctcgcc tacctcaagc
    16381 agttcccggt ggaggcgctc aagatcgacc agagcttcgt cgccggcctc tgcgagaacc
    16441 ccgacgatca cgccatcgtc gaggccacca tccagctggc cgctgcgctc ggtctcggtg
    16501 tgatcgccga gggggtcgag gagcaggcgc aggccgacga actccgtcgt ctgggctgcc
    16561 ggtcggtgca gggctacctg tactcgcgcc cgctcccggc agcgacgttc gaggcgcagt
    16621 ggctggggca gccggtgtcg atgccggtgt actagtagcg gaacggctcg ccggcgtctt
    16681 cgcgctcggc ggccttgacc ccgtacccga agatgagtgc ggggatcagg agcacggcgg
    16741 acgcgacgag gcaccccacg atgatgccgg tgacggccgg accgaactcg gtggcgaatc
    16801 cgatgacgaa cacgatcatg gccacgagga tcgtcaggta ccccgcccgt ttggccatcg
    16861 cgctccaccg ggcgacctgg gctcggcgga tcaggacggg atcatcggag gcggtcacgt
    16921 cgtccagctt gtcattcgtc ctcatcgaac gcctcctccg gaccggcccc cgagtcgccc
    16981 cgcgctcggc gcgccgctgc catggcgcgg ggatcgaggc gggagggatg gagaccgggg
    17041 ccgtgacggc gcgccggccc ctggcccagc ggcgcgatgc ggcggcggta gtcgcctccg
    17101 ccgtcattgc ggaggacacg accgcggagc agtgaggccg tgacgaagag gtaaccgaag
    17161 tgcaggaggg tgtcgccgaa cgagatgacc tggccggtgg ggagcgcgat cgtctcaccg
    17221 agccaggtca gggtgtcgtc gtcggtggcg acgtgtcgcg caccttcgag cttcacggtg
    17281 cctcgatcgg aggccttgat gatgtccgcc gtcaccaggg cgtcgcggct ggtgggcatg
    17341 ccccagttga tgacggtcgg gatgaggttg agggacaggc ctgcgacgac gagcaccatg
    17401 ccgccgaggc ggacgttcga gacggcgaat gcgagcagcg cgagggcgct gatgatctcg
    17461 agcacgacgg cgaggctgcc gtcgaggccg gcgacacgga tgacgagctg gaggaacaat
    17521 cctccggcca gcgccggcca gagctgaggg cggtactcga ggatccggtc gatccggccg
    17581 ccgtgggtga gccccacgac tccgccggcg acgatcgcga gcagcgtgag cagggcagcc
    17641 atgcccgatg agcataggcc gtgcaccggc ggcggcccgg cccgctgtga ctatcgtgcg
    17701 gcgcatgtct gacgaactcc tggcctcgag cgccgacggt gtgctcacgt tgacgatcaa
    17761 ccgccccgag cggcgcaacg cgctgtcgtg ggatgtgatc accggactcc gggccgcggt
    17821 cgccgcggcg aaggacgacg cgacggttcg ggccgtcgtg atcaccggcg cgggggagaa
    17881 ggcgttctgt gccggcgccg acctcggcgg tatggccgac aacgcgtcct acgccgaggt
    17941 ccacgatgcc cgcggcgaac tcgcccggct gttcctcgac ctctacgggc tcggaaaacc
    18001 gacgatcgcg aaggtgcgtg ggttctgcct cgccggcggt ttcgggctgg cgctggcgtg
    18061 tgacctcgtg atcgcgtcgg acgacgccac gttcgccacc cccgagatca acgtcgggct
    18121 ctggccctac atgatcacgg tgccgctcac gcggtcgatg ccgccgaaga aggcgttgga
    18181 gctcatgatg acgggccgcc gcatcgacgc tgccgaggcc gacgcgctcg gcctgctcac
    18241 ccgcgtggtg tcggcggccg acctcgacgg ggccgtggcg gagacggcgg cgctgctcgc
    18301 atcgaagtcg ccgtcggtca tgaagatggg ccgcgactcc ttctattcgg tgtgggggcg
    18361 aaacgccgcg gactcgttgg cccagctgca tccgctcctc acgctgaaca cgtcgctcga
    18421 ggatgcggcg gagggcatca cggccttcct cgagaagcgc gaccccgtct ggaccggccg
    18481 ctgatcgtcg agcggcgcta ggttgctgcg ccatgaccga ggtggacgag cgatacaact
    18541 gggggccgct ggtcgccgac ctggcccagc gcaaggagac ggccctcgca atgggcggac
    18601 ccgagcgagt ggagcggcag cgcagcctcg gcaagctgcc ggtgcgagaa cgcctcgacc
    18661 tgttcctcga ccccggcacc ttcgtcgagt acgggatgct ggccgaccac atggatccgg
    18721 gcctggcctc gaagggctcg ttcgctgctg acggctgcat caccggcatc ggcaacgtcg
    18781 acggccgacc cgtggcggtc gtcgcctacg acttcacgat catggccggg tcgatgggcg
    18841 aggtgaacga gcagaaggtg acgcgtctgc gcgagctggt gcttcgacag cgcatcccgg
    18901 tcgtgtggct cctcgactcc gccggcgccc gcgtcggcag cggcaccgga tccaccttcg
    18961 cgggcgccgg cgccctcttc cgtgagcagg tcacgctgtc gggggtcgtc ccccaggtcg
    19021 cagccctcat ggggcactgt gccgccggca ccgcgtacat cccggccctc accgacttca
    19081 tcgtcatggt gaagggcatc agttcgatgg cgttggccgg tcggcacctc gtcaaggccg
    19141 cgaccggtga ggacgtcacc gaggaggaga tgggcggctc cgacgtgcac accaaggtgt
    19201 ccggcgtcgc cgatctcgcc gtcgacgacg acgtcgcctg cctcgacgcc gtcaagaagt
    19261 acctgtcgtt cctcccgtcc aacaacacga cgccggcacc ccgcaaggag acctccgatc
    19321 cggccgaccg ccgggccgag gagatctacg agatcgtgcc caccgcgccg cgccgcgcgt
    19381 atgacatggt caaggtcgtg aaggcgatcg tcgacgacgg cgacttcttc gagatcaagg
    19441 cggcgtgggc ccgcaacatc gtgtgcgggt tcgcccgcct cgacggcgag tcggtcggca
    19501 tcgtcgccag ccagcccaag gtgctcggtg gtgccctcga cgtgaacgcc gccgacaagt
    19561 cggcccggtt cgtctggttg tgcgatgcgt tcagcatccc gctggtgttc ctccacgacg
    19621 tgcccggctt cgtcgtcggc accgtcgtcg agaagcaggg catcatccgc cacggcgcca
    19681 agatgctgtt cgccatcagt gaggcgaccg tcccgaagat ctcggtcatc cttcgcaaga
    19741 gctatggcgc cggctacttc gtgatgaacg gtcgtgccta cgaggccgac tatctcgtgg
    19801 cgtggcccac tgccgagatc gcggtcatgg ggcccgacgg tgtcgtgaac atcgcgttcc
    19861 gcaagcagct cgaggaagcg cccgagggcc ccgagcgcga caagctgcgc atcgagctcg
    19921 ccgagatgat ccgcgccaac atcgatccgt gggtcgccgc cggccacgcg ctcgtcgacg
    19981 acgtcatcga tcccgccgac acccgcgctg cgatcatcgc gggcctcaac gccaaccgga
    20041 acaagcaggt gttccgcccc tggcgcaagc acggcgtcat gcccgtctga cgctgtactg
    20101 tcgccgcctt cgggcggacc catcgatcgc cgtcgagcag aaggaatccc actcccatgg
    20161 acgtaaccgc agagatcacc gccaacgtgt ggcaggtgcg caccgaggtc ggcgccaccg
    20221 tcagcgaggg cgacgagctg gtcatcctcg agtcgatgaa gatggagatc cccgtcgagg
    20281 cgccgatcag cggcaccgtc acgtcgatcg cggtgaagcc cgacgatcag gtcaccgagg
    20341 gcgatctcgt tctcaccatc gaaccgtgac cggctcggtc accgccggta gcgaggccgt
    20401 cgaaccgatc cgctgcgagg tgacggaccg catcgcggtc gtcaccatcg accgcacggc
    20461 gcgccgcaac gcgctcaacc tcgacgcact gcaccgcctc gacgacgccg tggcgtccgc
    20521 cgtggccggc ggcgccgctg ccgtggtgtt ccgcggcgag ggagggcact tctgtgccgg
    20581 cgccgatctc aaggagctcg aggaccagtc gttcgacacc cgactccggg gtgtgctcga
    20641 gcgcatcgcc accgcgccgg tcccgacgtt cgccgcgatc tccggtgcct gcatgggcct
    20701 tggcatgcag ctcgccctcg cgtgtgacat ccgggtcgcg accgacgacg cctacttcgg
    20761 catcccggcg ggcaagctcg gcctggtcgt caaccactgg acgctgcagc gactggccct
    20821 gaacctcggg ttcggggcgg cgcgtctgat ggtgctcact gcggagacct tcacgagcga
    20881 cgacgcgtgg cgcctcggct tcacccaggt ccgcggtgat ctcgacgccg cggtcgcgct
    20941 cgcccgccgg tcggccgacc tggcgccctt gtcgctgcag ggctcgaaga tcggcctgaa
    21001 tctcgtggag cgtgcgctcg acgaaccgga gttcaacgcc gcgttcgagc gggcgtggaa
    21061 cagcgccgat ctcgccgagg gccgcctggc gttcgccgag cgacgcagcc cggtgttcaa
    21121 gggcgtctga gcccgacgga ccgagtgcgc ctgtcggagg gccgaggctc gctgaacaga
    21181 gttcaaggat tgtcgcaggc cggtcgacac tcatgcagac ggtggccgct ggggacgccg
    21241 tcgaccgcag gagttgacac gcatggcttc ggcccgattc ttgaagcgat ccttcgccac
    21301 cctgctcacc gtcctggtgg cgggatgctc agcgccgtcg tcgctgggca acccacctcg
    21361 catgccggtg gccaagccgg tgggtgcacc cacgacgtcc tcgaccgcgc cggcgactcc
    21421 gtcgtcaccc acgtcgcctt cgaccaccgc cgcgaacccg acccaggcga actgcgccgg
    21481 cagctacgag cggttcttcg cctgcgatgc gtcgtggaac cgcccggcca gccacttcgg
    21541 cgacgcgacg ggccgcctcg cgtcctacgg cacgcggttc tggaagtacg gcggcgactt
    21601 cgtcgggggc aactggaagc cgggcgacat caacatgcac ttccgggact attcggtccc
    21661 gatctacgac ctccgtaccg cgatcaacgg ccggtaccgc gcctaccagg cgatcgagta
    21721 ccagaagatc tcgatgttct ccccgggcac gcgcaacgtg accatcggcc agatcatccc
    21781 gtacaacccg gcgtggatcc ccggtaccgg caccgatcgg ctgatgatcg ccgtgaaccc
    21841 cgataccggt gagacctggg agttcggcgg tgtgaacctg cccctcggag cgttcggcgt
    21901 caaccagtgc tcagggttcc tcgggcccaa cgccctggcg ccggtgccgt tcgatccggg
    21961 caaccccacc cacatttgct tctacgggat ggcgcactat tacaacctct atgccgccac
    22021 agacgggacg acgatcgccg ggcgggggat gggcatcaac aagatggcgc tcctgacccg
    22081 cgccagtgag gtcgcccgtg ccgtcgccaa cgacacgtcg atcgggcacg cgctcgagat
    22141 gacgttgccg gcgacgatgc cgggcgagcc gatttgtccg gtcaacgacg gtcggttcac
    22201 cgatcccggc gccggcgtca gctgcggcac gttcgtgtcg cccgccacgt tcgtcgaacg
    22261 cgcccagggc acacgggact ggtgtgggcc ggggcaacgg tcgatccacg acccggccct
    22321 cccgtcgacc tggttgcgaa cggtgcccga gggcttgagg atccgtctgg agatgaccga
    22381 cgccgacatc gtggcgtggc tcgacgatcg tgcccggacc aacccggcgt acggccgggg
    22441 gcagcccctg cgggcgacgg cacaggcgtt cgcccgtacg ttcgtcgagt acggggcgat
    22501 cgtcgcggaa acgggatgcg ccgggatcgg catcgagacc gagggtttgc aggggccggc
    22561 gaagtcgacc tgggccgagc tcggcatcgt cgacccgagc gggacgaatc cctatcccgc
    22621 tggtgatctc ctctacgggc tgatcaccga ggctcgcatc cgggtggtgc agccgcccga
    22681 gccgcgctga gcgggcgcgg gcggctcagc gctcgaccgc ggcgaggatc gcgggctgga
    22741 cgcaggtgcc gatcgcttcg tacgcggcgt cgttgggatg cacgaggtcg atcgtcccga
    22801 aggcgtcgat gaagttccag taccggggcg ccaggacgtc gctggccggc gtggtctcga
    22861 gcaccgggga gcaatcgacg accgctccgt ggaggttgcc cggggttgcc atccacgcgt
    22921 tgatcgtctg acgggcgacc tggtggacgg tgtaccagtc ggccgcgttc gagaccggcg
    22981 tgatctcgag gaagaccgct tcgacaccgc gggcatgcag cgcctcgtcg agttcgccga
    23041 tcgactcgat cacggcctcc gccgatcggc cgtagctgag gtcgacgtga cccccggaga
    23101 tgacgacggc cccgacgtcg ttgccgcagt cgtcgaggtc gtcgaggatc cgactcccga
    23161 tggtcccgta gtcgggatcc ggcgtgaggt tgccggtgta cgcggagccg ccgcgggcgg
    23221 tgttgtacgg ggtgagctcg ggaggcagtg gtacccagcg cggccattgg gtcacgaggc
    23281 tgtcgccgcc gtagatcacg atgccgtcgt cgaaggggca ccgcggcctc gtcgaggtca
    23341 ccgatggtgg accgaccggt tgtggttcac acgccccggt cagcatcacc atggcgatca
    23401 ccaacatcgt cgccgccgct cgtcctgccg caacccgcat gtccgccccc cagaagcttc
    23461 gtgatgttgg tcagaacagt atctggatcg cgaagaagtt tcgaatgtag tggctcgggc
    23521 ccgttcgcgt gtgcgggcgc gctgcaccgg ccaggatcgc ggtactcggt tctccgggag
    23581 tgagatcggg ttcgcggcct actggccgga gccgcggacg gtgcccttgg tcttgccgcc
    23641 ggtcacccgg tagacggcgc acaggatcgt gcggtccttc ttggtcgccc acgactgctc
    23701 cgacggccac cacgactgga tgtcgtattc gctctcttcc cacttcaccc ccatgaactg
    23761 ggggaacgcg ccgacgcact gggactcggc gccgttcgcg accgccaacg ctcccgggta
    23821 gggggcgccg ttctgggtcg gggtcgctcc gaggtcgagc ttggcctggg cgtacacctc
    23881 gtaggtgtgg ggatcctcac aggggatgac catgacggcg tacgtctgct gggcggggtc
    23941 gggcacgtcg tcgaggcact gaccggcgat cacggcctcg gtcggcgtga gcgtcccgac
    24001 gaggggggtg ctcgtcgtgg tcgtggtcgc cgccggagct ttgccctcgt cgtcggcgtc
    24061 cttgctgccg ctgcacccgg tgaggaggac ggcggcgacg gctgcgcacg caagggccgg
    24121 cagacgtcgt cgggctgggg acccaccggc gagaatcacg gcccgcaccc tactgggcgt
    24181 cagtacgacg gcaggaagcg gtccatgtgc acgaagcaga accagagcgg agcgaccgtg
    24241 gcgaccgtcg tcacgatgta gatcggccac caccgcatct cgcgacgacg gaacagcgcc
    24301 gcgagcgcca tcgaaccgag ccagagtgcg atcacgatgc cgccgaagag gagggcggga
    24361 gcgagttcac cgggatcccc ggcgatgaga tcctcggtcg tctccaacgg tggggtcacg
    24421 gtctgggcgt cgtcggtgcc gtccgctccg gtcgcggggt cggtcgtcgg caccgtggtc
    24481 gtcggtgcct cggcgggctc ggcgacgagc ttggcctgca cgatgatccg gagcttggcg
    24541 gagtacttcg ggtggcaggc ggtgagcgtg aggcggttgt cgtcggtcgg cgccaggacg
    24601 ctcacgtcgt tgggcttcac ggcgaaccat cccggacccc aggtgccgcc ctcgtcgccg
    24661 gcccgtcgct ccacaggcga ctcgagcacc tcgtagacga acttcccctg cagtgtgtac
    24721 gtgatgatct ggtcgccggg cttcagttcg tcgatgcggt tgaagggtgc gccgtaggtg
    24781 gtgcggtgcc cggcgatcgc ggcgttgccc gggttcccgg ggagcggtgt gctcgggtag
    24841 tggccgggcc ccttgcggag atcggccttg gccacgcctt cgacgaacgt cttggatcgc
    24901 agcccgatcg ccgggatctc gatcttgccc tcggggtcgc cttccttcgg gatctccgcc
    24961 ggcggcgcct tggtcgggtc gatcgaggag agcttcgccg tgatcttctc gtcgatcgcc
    25021 gcggcgtctt cctcctccgc gtcggtcttg gccgtgtcga cgccgaggct gtggccgagc
    25081 gaccgcgtga ggtcctcctg gtggccggac tcgtcgagcc ccgtgcccca gagctggtag
    25141 ccgacgaaga ggaggaggac gatcccggag aagatgaggg ttcggcccag accgcccaag
    25201 atgcgattga tcattccgga ccacggtagc ggtgagggtc ccgcgatcgg tgggcaggac
    25261 cggggcgttg gtgggcaaaa ccgaccaacc tgtagcgttc ggtgcgccat cgggcacccg
    25321 ccccggccga tcccaggtga cccgatcccg atgcccctcg accctgtcat ccagctccac
    25381 ggagcggtcg ccctcctcgg gcagttcccc gtgctcgccg gcgtggacct cgaggtcggg
    25441 gacggtgaac gggtgttgct ccggggcccc aacggcgcgg gcaagacctc gctcctgcgt
    25501 acgtgcgccg gcctcattcc cgtgtcgtcg ggatcggcca ccgtcctggg ccacgacttg
    25561 cgggccgatc gtcgttccgt gcggcccctc gttgggctga tggcgcacac gaccggcctg
    25621 tacgacgagc tgaccgccgc cgacaacgtc cggttctggg ccgaggcgtg ccgggtcgac
    25681 cccgccgacg ccgcgggtgc catggaccgc gtggggttgg cgacgcggct ccgcacggtg
    25741 ccggtgaacc gcttgtcgac cggccagcgg cggcgggtgt cgctcgcagc gatgatcgtg
    25801 aggcgcccgc gcctctggct gctcgacgaa ccccacgccg gcctcgacac ggtgggccgt
    25861 gacctcgtgg accgcctcat cggcgacgcc gcggctgccg gcgcgaccgt gctgttcgcg
    25921 tcacacgacc tcgaacgcgc cgacgcggtg gccgaccgca ccgtcagcat cgtgggcggc
    25981 gccgtcgccg aggacaccgg gccacggccc gccgatcgca cgggggccga cgatgtggca
    26041 tgacgtctgg ctcgtctgtc gcaaggacct ccggatcgag gcgcggacgc gggtgacgct
    26101 caaccacgtc gccccgttcg tcctgaccgt ggtgatgttg ttcgcgttcg cactcgacgc
    26161 cgacacgacc ctgctgcgtc gggcgacggg gggcctcttc tgggtcacgg tcctgttcgc
    26221 cgcgaatgcg atcgcccagc gctcggcgtc ggtcgaccgc cccgacggcc tgcccgacgc
    26281 cctccgcctc agcaacctct cgcccgccgg gatgttcctc ggcaagacct tgtcgctgtt
    26341 cctccaactc accgcgaccg aactcgtgct cgcggtcgcc atggtcttga tgtacgacgt
    26401 caggctccgt gggatcgccc tgatcctcgc ttcgattccg ctcggcacgc tcgcgatcgc
    26461 agccgtcggt tcggtctacg gacccctcgc cgcgggcctg caggggcggg agtccatcct
    26521 gcccctcctg atgctcccgg ccctggctcc ggtgctcctg gcggcgacga acgcgttcgg
    26581 tgtggcgttc ggcaccacgg tcggccctgg ctggcggtgg gtggtgatgt tgggcatgct
    26641 gggagcgata tatgtcgccg ccggaacggc gacgtcggcg gcgctgctgg aggagacatg
    26701 aggatcctcg ggcagtgcct cgaacggaac gagtgccgat gaccgatcag acctccgcgg
    26761 ctcccgccgg gacggccgac accgccaagc ccgtcgccat gtcgacgtcg tcgcgaggca
    26821 cgaaggcgct cggcctgctc acgctcgtga gcgcggtgtt cacgatcctc tactcgttct
    26881 ggttctcgaa ggccgacgtc gagctgggca acaccgtccg catcctctac gtgcacgtgc
    26941 ccaccgtgtc gatcgcctat ctctgcatgc tggtcaacgc cggggcgagt gcgttctacc
    27001 tgtggaagcg cacggcgttc cccgacctgc tcgccgccgc caccggcgag atcggtgtgc
    27061 tcctcctcgg cctcacgatc ctcgccggga tgatgtgggg caagccgacg tggggcacgt
    27121 tctgggagtg gggcgacccc cgcctgacct cgacgctgct gctcttcctc atgtacctcg
    27181 gctacgtgac ggtacggaac atcccggcgc cgcgttccgc ccgcaatacc cgctcggcgg
    27241 tcatcggcat catctccgcg ctgctcatct acccggtcaa gatgtcgacg gagtggtggg
    27301 gctcgctcca ccagggccgc accatctcgg caccgggcgc gtcgaagatc cacggtgctc
    27361 aggagttcgc gctgtactcg ggctttctga cgttcatgct cttcgcggcc tggctgacca
    27421 tccaccggtt ccgggtgggt tggctgcagg accaggccga cgcgaccgag ctcgacgctg
    27481 tcattgccga ccgtcgcgcc gaggccggcg ccgaacagtt cggactcggg gaggtccatt
    27541 gatgatcgcc caacagatcc agaacggcga ggcctggacg tacgtgggaa tcacgtgggg
    27601 cgccaccgcc gtggtgctcg gcgggtacgt cctgctcata ctgcggcggg gcaaggccct
    27661 ctcgaagcgg gtcccggagg acaaacgccg atggatgtga gctcgatcga cgatcccgag
    27721 tccgtcgacg ccggaccgct cgacctcaca ccccgttcgg ctccgaccgc gtcgtcgggt
    27781 ccggcggtcc gccgacgcaa gatcgtgccg atcgtgctcg gcgtggtggt cgtggccgcc
    27841 atcgcggtgc tgctgatcac ccagctcgcc ggcgcggcga cgtacttcta caacgtcgac
    27901 gacgccgtcg cgaagaaggc cgacatcggc gacaagcgca ttcggctgca gggcaacgtg
    27961 atcaaggggt ccgaggtcgg tcgaacccag gacagtgtgc agggttacgt catcgcctac
    28021 aacggcgtgc aggtcgaggt cgagcagtcg ggcgacgttc ccgacctgtt cggaccgtcg
    28081 atccccgtcg tcatcgaggg ccgcttcaag ggcgacctct tcctgagcga ccgcgtgctg
    28141 gtcaagcacg acgagaccta cgacgaggac aactccgacc gcatcaagga tgcggagcgg
    28201 gacgcggaga agaacgtgtc gtcaaccacg atcccgaccc cctgagctcg gctgtgtccg
    28261 tcgccctcgg aaatgccgcc atcaccctgg gtctggtcgc ctgcatcatc ggcgtcggaa
    28321 cactcgtcct cggcattcgc cggggcgacg agaagctgct gatctggtcg cgaccggtgt
    28381 cgaccgtggc gctcgtcgcc gcggtcgccg ccgtcggagt gatgcagatc gcgctcctca
    28441 ccaacgacac gtcggtgaag tacgtcgccg acgagggttc gaagcggacg ccgtttccgt
    28501 tcgacatcgc gacgatgtgg tcggccctcg aagggtcgat cctgctgtgg ggactgctgt
    28561 tgacgatctt cacggcggcg gtcgccgcga agttccgtcg ccggctcacc gacccgctcg
    28621 tcgcgtgggc gatggtcgtg atgctgacgg tcaccgggtt cttcttcacc ctgatgctgg
    28681 ccgtcgcccc ggtgttccgc tcgttcgacg cacccgtcgg cttcgacggc cccggcccca
    28741 acccgctgtt gcagaaccac atcctggtcg ccttccaccc gccgctgttg tacctgggct
    28801 acgtcgggtt cacggtgccg ttcgcgttcg cgatcgccgc cctcgtcacc ggtcgcgtcg
    28861 gtgagggctg gctcgccgag acccgccggt ggacgctctt cgcctggggc ttcctcaccg
    28921 cgggcatcgt cctcggcgcc tggtggagct acgaggtgtc gggatggggc ggctactggg
    28981 cgtgggaccc ggtcgagaac gcgtcgatcc tgccgtggct cacgggcacg gcgtacatcc
    29041 actcggtcat ggtgcaggaa cgtcgcggca tgctccgggt gtggaacctg accctcgtgg
    29101 tcgcaacgtt cagtctcacg attctcggca cgttcctggc gcgatcgggc gtcgtggaat
    29161 cggtgcacgc attctcgagc ggctcgatcg gcccgacgct gctcgggttc ttcgccttca
    29221 tcgtcgtcgc ctcgatcggg ctgatcgggt ggcgcggcga ccggctgcgg tcgcccggag
    29281 cgatcgactc gccgctctcg cgcgaaggag cgttcctcgc caacaacgtg ctgctcggtg
    29341 cgttcgcgtt cgtgatcctc ctgggcacgg tgttccccct gatcgtcgag gcggtgaacg
    29401 acgaccggat gtcggtcggt gccccgttct tcgaccgaat ggcgatcccg atcgggctcg
    29461 tcctgctctt cctcatggcg gtcgctccgg tgctgccgtg gcgatcggcg agcaccgacc
    29521 tgttgtccaa acgtctgtgg tggccgttgt gggcatcggt ggtcgcgctc gggttcgccg
    29581 tggccgtggg cgcccgcggc gtcacctcac tgctgggctt ctggctcgcc gggttcgctg
    29641 ccggctcggt cgcccgccaa ctcacgctcg ccacccgtcg ccagggttgg cgtggcctcg
    29701 tcgggcgggc caacggcggc atggtcgtgc acctcggtgt gctgatcatc tcggtggcgc
    29761 tcgtcgccag ccagtcctac accaagcagg gcgagttcac gctggagcgt ggcgagacgg
    29821 tgcgggtcgg tggacacacg ttcgagtacc tgtcgagcag cgtcgtcgag ctgtccaacc
    29881 gccgtgagct ccgaagcaag atccgcatcg acggcgagga cgtcttcgag ccgtcgatga
    29941 accagtacct ctcgacgggc atgtcgatcg cccgtccgtc ggtgaaggtg acgccgacga
    30001 ccgacctgta cctcagcatc acgcgcccgg cgagcgccga ccgacccgac atccgggtgc
    30061 gcatcgtggt cgagccgctc atcgtgtggc tgtggatcgg cggcttcgtg atggtggccg
    30121 ggaccgtgct gtcggccttc cccggacgcg gtcggcgcaa cccgctcgat ccggtgtcga
    30181 gcccgatcgg tcacgaccga gaccccgagc ccgtcgcggc cgacgaaccc gaacccgaac
    30241 ccgacgcgga cgtcgacacc gagcccgagg gggtgccggc gtgaagcacc gctcggcgct
    30301 catcgccggc gtcgtcggtg tggtggccgt ggcactcatc gtcctgttcc tgacggcgga
    30361 catcgacggc aacccgctcg acaccgacac caaccagctg gtcggcaagc cggcgccggc
    30421 gatcgacgcc accgacaccg agggccggcc gttcgccgtg tccgactacc gcggccggtg
    30481 gctcctcgtg aacttcttcg ccacctggtg tgccccctgc atcaaggagc accccgagct
    30541 ggtggcgttc agcgaggcgc actcacgcct cggcgacgcc tcggtggtga gtgtcgccta
    30601 caacgacgag cccgatctcg tcaacgagtt cttcgagcgc aacggcggtg actgggccgt
    30661 catcgccgac gacaagagcg acttctcgat cgactacgcc gtcgtcggcc tgcccgagtc
    30721 ctacctggtc gaccccgacg ggatcatcgt gcacaagttc atcggcggca tcaccaaggc
    30781 cgaggtcgag gccgtaatgg ccgaccaggg cccggcgacg acgaccacgg tggcaccgtc
    30841 gtgatgaacg gtcccgcatc ccggcgtacc cggttgctcg ggtgggcggg cgcgctcgtt
    30901 gcggttgtgg tcctcgtggg cctcggcgcc cgtacgaccc cgacctcggg cacctccgaa
    30961 gaccgcgtct acgcgctcgc cggccagatg aagtgtctgc agtgcctggg cgaatcggtc
    31021 gccggatccc aggccgacat cgccgtgaag atgcgcaccg agattcgtgc ccagatgcgc
    31081 aagggccgga ccgatgacga gatcctgagt tcgttcgccg acacctacgg ccagcgggtg
    31141 ctgctcaatc cgtccggcga cggcgtggcg tcgctcgtgt gggtgatccc ggtcgtgatc
    31201 gccggcctcg gaaccgtcgg gttggcgctg gccttcgctc gctggcggcg ccagcgcaac
    31261 gacgccggga cgaccgaggt gaccgacgag gaccgcgccc tcgtcgacgc cgccctcgcc
    31321 gacggagcca acggtggctg acgccgaggt accgcaacgt acgaagcctt cgacggacga
    31381 gggagcgagc ccgggcagag gtggccgacg caaccaggtg aatctcgatg agttggcgcg
    31441 ccgcaccgag gagcgcgact tcctgctggc ctcgatcgcc gacctcgatc gtgagcacgc
    31501 ggccggagac gtcgatcccg acgactacga cgaactccgc cgcggctacg tcgcccgggc
    31561 cgccgaggcc atccgggcgg tcgacgccga tgaggccaca ctgcgcagtc agcgcgatgc
    31621 ccgacccccg cgccgcggcc gggcgttgat ctgggtcggc ggagtggccg tgttcaccgt
    31681 cgtcgccggc gtgctgctcg ccaacgcgct gggctcacgt ggccagggtg ccggtctcac
    31741 cggcaccgga tccatgcccg gcgatcaggc cagcacgtgc cgatcgctga gcatgagcaa
    31801 gccggccgag ggcatcacct gctacgacaa ggtcctcgag gaccaacccg acgacgtgga
    31861 cgcactcacc tatcagggct gggccaaggt ccggtccggc gacgtcgaag gcggctcggc
    31921 ccagttcgat cgggtcatcg agctcgaccc gggcttcgcc gacgtccacg tcttcaaggc
    31981 atcagtcctg aagaacgccg gcgacttcgt gggggccaag gccgagctcg accgggtgta
    32041 tgcgctcaac ccgtcgccca tcgtcacgtc cacgctcgaa cagatgggcc tcgacaccga
    32101 gatcgcagtc ggcctgctcc ccgccgacgt gcaggcgtgt tggaaggacg agaaggcggc
    32161 gctcgacgcg acggttgccg tcacctcgaa ggccgatgcg gagatcgacc ggtcggcgta
    32221 cgccggagcg cttgccgacg tggccctgtc ggccaagtgc ctcgacacgg tgctcgcagg
    32281 ccgcccgacc gacgccgacg ccctggtgct ccgatcgctc gccgtgggcg tcctcggact
    32341 gatcgacggc tcgacctacg acgcggcgga ggccgacgcc accgcggcgc ttgccgcccg
    32401 cgacggcgat ccggcggcct tgctgttgcg ggcgttgtgg cgcgacaacc tcggcaacgt
    32461 cgagggtgca gcggccgatc tcgacgcgct cggcgaccag cgggtgtcgc cgctggtgac
    32521 cgcctatgtc gaggtcgcgc cggtccgggc ccaagtcgag gccgaccgcg ccgcggcccg
    32581 gtcgacgacc acgacgcgtt gagccgtcgt caccccgtca cctcccccta gattcatccc
    32641 ccatgaagat cctcgtggta ggtgccgggg gagtgggcgc tgcgttcgcg gcgatcgctt
    32701 cccgtcgttc gttcttcgag cagctcgtgc tggccgacat cgacgtctcc cgcgcccagc
    32761 gcacggtcga ccgcctcggc gacccgcgcg tgactgcgat ctccctcgac gcctccgacg
    32821 cgacgtcggt cgccgcggcg atcggtgcgc acggctccac gatcgtgctc aacgcggccg
    32881 acccccggtt cgtcgtgccg atcttcgacg gttgcttcgc cgccggggcg acctatctcg
    32941 acatggccat gtcgctgtcg cacccgcacc cgagcgatcc ctacgcccag gtcggcgaga
    33001 agctcggtga ctaccagttc gaccggtcgt cccagtggga ggagcggggc ctcctggccg
    33061 tcggcggtat gggcgtcgag cccggtctcg ccgatgtgtt cgcccgctac gccgccgacc
    33121 acctcttcag cgcgatcgac gaggtcgggg ttcgcgacgg ttcgaacatg gtggtgaagg
    33181 gttacgactt cgccccgacg ttctcgatct ggacgaccat cgaggagtgc ctgaacccgc
    33241 cggtcatctg ggagaaggag cggggctggt tcacgaccga accgttctcc gagcccgagg
    33301 tgttcgtgtt ccccgagggc atcggccccg tcgagtgcgt caacgtcgaa cacgaggagg
    33361 tgctgttggt cccgcgctgg gtcgactgca accgggtcac gttcaagtac ggcctgggcg
    33421 acgagttcat cgacgtgttg cgcacgctcc acaagctcgg cctcgactcg acggacccgg
    33481 tgtcggtccg cgggcagcag gtctcgccgc gcgacgtggt cgccgcgtgc ctcccgaacc
    33541 cggccgacct gaccgacgag atcgagggca agacctgcgc cggcacctgg gtcaccggca
    33601 cgggtaccga cggcaacccg ctcgaggtgt acctgtacca cgtggccgac aacgccgaga
    33661 cgctggcacg cgacggcgcc caggccgtcg tgtggcagac cgcgctgttc ccggtcgtgg
    33721 cgatggagct catcgccgcg ggggtctgga gcggcgccgg ggtgaagggg cccgaagcgt
    33781 tcgacgccgt cccgttcctc gacatggtga aggacgacta cgagtcgccc tgggggctcg
    33841 agatccgctg aggccggtca ggcgtcgtcg gcgttcagcc gttcaggcgt tcaggcgttc
    33901 aggcgttcat cgctcggacc agctccacga tctcgtcggg cgccttcgac ggcgacccgg
    33961 catcgacggg aggttggggg tcgtactcga tgccgagctg gatcgcctgc gccacctcga
    34021 tgccggccag ctcgatcacg agccgcaacg ccatgtcgat gccggccgag acgccggcgc
    34081 ccgtgatgac cttgccgcgc acgacgacgc gctcctcggt cggatgcgcc ccgaactcgg
    34141 cgagttgttc gtaggcgatc cagtgggtgg tggcgtcgag gccgtcgagc acgcccgccg
    34201 ccccgaggat gagggcgccg gtgcagaccg acgtcgtcca cgtcgtggcg gggtgtgccg
    34261 accgcaccca ttcgacgagg gggttgccgg ccgcggccat cgaccgggtg acgacgccac
    34321 cgggcaccac gatcacgtcg ggcgaggcga cctcgtcgta ggtgtgctcg atctcgacgt
    34381 gcagcagccc cttgtcgtcg gtcaggcgac cgcgctccgc gccgaccagg acgacctcgg
    34441 cacccggcag ccacgtcagc acctgatagg ggccgatggc gtcgagcatg gtcatcccgg
    34501 gaaacatggc gatcgcgatc tgggtcatgt cgtgtggtcc ttcttgaaat gggatcggta
    34561 ggaggcgggg gtcgtggcca ggcgtcgctc gaacaaccgg tgcagcgtcg aggcgtcgcc
    34621 gactccacac cgatgggcga cctcggcgac cgtcagatcg gtcgtctcga ggagcgtgcg
    34681 tgccaggtcg acgcggcacg actcgacgta ccgggccggt gtcgtcccca cctgttcgcg
    34741 gaacagccgg gcgaagtggc gtggactcat ggatgcgacg ctcgccagcg tctcgacgga
    34801 gcagtcgatc gacgggtcgg cggcgatccg gtcctgaacg atgcgaatct cgtcgcgtcg
    34861 cgccgtcccc gagctgaccg gggtcgagaa ctgcgactgc ccgccggagc ggcgcaggta
    34921 caggaccagc cactgggcga cctggcgggc gatctcgtcg tcgtggtcag cggcaaccat
    34981 cgcgagcgcg aggtccatcc cggcgctgac gccggccgac gtccaggtct cgccgtcgca
    35041 ggtgtagatg aggtcacact gcacgtcgac ctcgggatgc ttcgctgcga gccggtccgc
    35101 cgaggcccag tgggtcgtgg cccgtttccc gtcgaggagc ccggtcgccg ccaggacgaa
    35161 ggcgccggta cagaccgagc cgacgcgtcg ggtgacctcg gcgacgccgc ggaagcgggt
    35221 cagaaactcg gggtcggcgc tcgtctcatg gctgcccctg ccaccggcga ccaggagcgt
    35281 gtcgggaggg tcggccagca gttcgccgag gctccaggtc gctccgagtt cgatgccgct
    35341 ggagcagcgc accgggtgcc cgtcgatcgt ggcgaccccg atgtcgtagc cggggcgctc
    35401 ggcgctgcgc agcacgttgt cggcggcgct gaacacctcg agcggtccgc agatgtcgag
    35461 cgagtgcgcc ttggcgaacg cgaccacggc cacgcgacgc gtcgacgggg ctcggtggtg
    35521 gggcatggcc ccatcctccg tcagcaaccc gacgtccgca atgacgagga tcttgcactt
    35581 tcagccatgc ccgtgatgtt tcgcgggaag ttgtccccga caggtgggga caacttcccg
    35641 caaaaccggt gggtggggcg ggcgactacg ggacggggac cgtgaggtcg cggtaggcga
    35701 cgatgcccgt cagcacatcg cccagcgccg cctggagctc gtcggtcgcc tgagcgaact
    35761 tggccttcac cccggccggg tcggcgttga aggagtcggg ggtgatgccg ctgacgagcg
    35821 cggcggcggc atcgacgtgc gccttggccg agacctggag cgcctcgatc gccgacgtat
    35881 cggcgccggc cgccttcttc tcggcgatca gcgggtcgaa cttgtcgaag gcgttgccga
    35941 actcgtcgat gcgtaccgag gcgaacagct gcgggatgac gacggcgtac acgcggttgt
    36001 cggcgtagat tgccgagcag gccgccttga tgccggcctt gcccttcgcg ttgttcacgg
    36061 cgggccggtt cacgttcacc aggttcgcct gcaccccgtc gatgccggcg atctgcgccg
    36121 ccttctgatc ggcagtgagg cgcttgacct tctcgatgcg ctgctcggag atgtcgagga
    36181 tgaagaggcg ctcgtcgatc gcctggttgc acttggcctt cgtgtaggtc gcaacgggcc
    36241 cgctcgtcga tgctccggcg ggagcgacgg cgacgaggcc aaggctcgca gcggtgagcg
    36301 aggcgacagc agcgagccga acggtggtct tcatgggaac tccttggggt ggtgggtaca
    36361 ggtcagttat cggcccgacc tgttaggaag atgtgagctc gagatgaggg agtcgaagga
    36421 cgatgacggt acggatcggt gggggacagg gcttctacgg tgacgggcac ctccccgtcg
    36481 cagcgttgct ggccgacggc atcgactacc tggtgtgcga ggccctggcc gagctgacgt
    36541 tggcgatcct gcagaaggat cgccaacgcg acgagtcgct cggctacacc cgcgacctcc
    36601 ccctctatgt tgccgccgcg atcggcgaca tcctcggggg gcgaacgaag ctgatcacca
    36661 acgccggcgg catcaacccg atcggcgccg gtcgggccgt ggtcggcgcg ctcgcggccc
    36721 acggggtcac cggggtgaag gtcgccacca tcgtgggcga cgacattcgt ccgcacgccg
    36781 aaccgctcgg cctggccgcc gacaccctgt tcgccaacgc ctatctcggc gcccgcccca
    36841 tcgtcgaagc cctcgagcgc ggtgccgaca tcgtgatcgg cggtcgcatc gccgacgcat
    36901 cgctgttcct cgccccgctc atccacgagt tcggctggtc gcccgacgac tgggaccgcc
    36961 tcgccgccgg cgtcgccggc gggcacctcc tcgagtgctc cgggcaggtc accggcggca
    37021 actactccgg cgattggtgg aacggcctcg atcccgcatc gatcggcttc ccgatcggcg
    37081 agttcgaacc cgacggctcc ttcgtcgtca ccaagccggc cgggtcgagt ggtcgggtca
    37141 cgttcgacac cgtgcgcgag cagctcctct acgaggtgca ggacccgacc gcgtacctca
    37201 accccgacgt gaccgtcgac ctggcctcgg cgcacctcga cgatctgggc aacgaccggg
    37261 tgagggtcag cggcgtccgg ggtgcagcgg ccccggccac ctacaagggc ctcgagtgca
    37321 cgcccggcgg atggtcgaac gagttgcgcg ccacctacgg gtggcccgat gccgaggcga
    37381 aggcgcgggc ggcggtggcc tacctccgcg gccgcgccgc tgccatcggt atggacgtca
    37441 aggagtggca cgaggagtac ttcggtgtcg acgcattcgg tgggccgacg gtgcccgccg
    37501 ctccggccgg ctgggaacct cccgaggtga tcgctcgcct ggcctggcgc tgcgacaccg
    37561 ccgaggaggc agggcgtctc ggccgcgagg ccggtctcct cggcctgtcc gggccgccca
    37621 tgatcgccgg agccggtcgg gcccgcgacg gcaagccgtc ccagttgttg tcgctccggg
    37681 ccctggccgt ggatcgcgcg ctggtcgacg ctcaggtgcg cgtcgagatc accgaggtct
    37741 gaccgtcgat cagcgcatcg gttcgtaggt gccggtggcg aagcgctcgc catcccactc
    37801 gagcgaccag agcgatccct tcgagcatcc ggccttgccc ggcacctcca tgccccggcc
    37861 cttggcgcgc cggacgagct cggggatcac gtcgccgtgg gagcacagca cagcgacctc
    37921 atcgtgttcg acgagcgctt ccacgagcgc ccacgactcc tcgatgtcgg tgccctcgaa
    37981 cagccgggcg tcgatgtcga cagcgaggcc gagcgacgac gccaacagct cgaccgtctc
    38041 gacgcaccgt gccgcggggc tcgccacgac ggccgtgatc tccaggccgg acaagcgctc
    38101 ggcgagctgc acggcctggc ggagtccgac ggcgtcgaga tggcgttcgt tgtcggcggg
    38161 gtcggcgtcg tcgcggacac cggccgagcc gtgccggacc aggtacagca tcacgatcca
    38221 gacgctagcg ccggcccgcg gggctccgga ccgacgtcgg gtgatcctgc ctcttgggag
    38281 tcgacagccg attcgctagc tttcgggacg gcaggatcgc gggggtggtc cgccggagtc
    38341 gagggggagc gatgcgggga acacgacgga cggtcgccgc gacagccctt gccgtcgtgg
    38401 cggtgctggc cagcggttgt cccgtcgagc cgacggggcc gacggacccc accggcccgg
    38461 tctggacacc gcaccacgcc ggcggcccgg cggtcggcgg cgccacgccc tgggcgatcg
    38521 cgatcggtac gaccgaggac tggtacgccc aactcgatgt cggcttcccg ctcacggcgc
    38581 cgcccacggt gtcggtgttc ccccgcacgg gccccgacgg ccactcgctc ggcgctccgc
    38641 agacgttcac cgccaacatg atcggactcg gggccggggt cctcagcgag catctgctcc
    38701 tgaccggcgg cgacccggcg tcggggtcga ccgccttgca gttcctcgcc gaaaccggtg
    38761 ggacgtggag catcgccggc tccttctccc tcgagtcggg cgagacgatt cgcggcatga
    38821 ccgacgacgc gctcgtcacc cgcgccgtcg gcgccagcgg ggcggaagtg cgggtctaca
    38881 cactcacccg cacgacgggt tcggtcaccg cgacgctgac gcagaccttg cgtcctccgg
    38941 ccgagtgggg ccctggctac ggaacctact gggggagcgt cgtcacgctc gacggtggac
    39001 tgctcgccgt ccacgcccac aacgacagca tcggcgtgcc cgaccgggtc ggcgtctacc
    39061 agtcgtcggc cgggtcgttc ggcctcgtcg ccacgctcga acgccccgag ttcggtgcgt
    39121 tcggcatccg gctcgcgatc gacgatcaac cggcggtcga ccgcctggtc atcggcgggt
    39181 atcccaccgg ctccgccgag gtggtgttcg ccgagtacac gtccaccgcc ggcggcacgt
    39241 gggccgcggt gagcacgatc gagccgcccg ccgccgttcc cggcgctgcc gtcggccaga
    39301 cgttcggcca gggcgtggcg ctcgacggcg acgtgttggt ggtgaccgcc gtcgacgaac
    39361 ccatggcgac ccccggaccg ggcggcgccg tccgcgtcga gtaccgcccg ctcgtctacc
    39421 ggctcggagc cggtgggtgg gagtacgagg cgacgctcag cgtgctgtca tcacccgtcg
    39481 acaccgacgg gctcaaccgg ggcctgatgt cgatggtcgt cgctgatcac cacatcgtgg
    39541 gcaccaacgg ggtgtcgttg cccgggggat gctcgtactg cttcggcctt cgcttcgagg
    39601 cgtggcgctg ggaccgcacc cctgcctgac ccgatcccgg tctggcagac tgcgcccatg
    39661 gcgcgactgc aactgcggga cctgtgtggg gttcgctcgg gtgacaaggg cgatatcagc
    39721 gatctctcgc tgttcgccga cgacgaggcc gcgtacgagg tgatccgttc ggaggtcacg
    39781 gccgagcggg tcgccgccca cttcggcgac ctggtgcacg gcgccgtcgt tcgccacgag
    39841 gtgcccaatg tgtgggcgct gaagttcgtg atggatcacg ccctgggtgg cggcggaccg
    39901 cagtcgctgc gtgcggacaa cctcggcaag acgctgggtg gtgcactgct ccgcttggag
    39961 atcgacgttc ccgacgacgt ggccgcggcg gcgccgcgta acggtcgccg cgccccgttg
    40021 ccgaactgac atgggcccgg tcacgcggtt ccgagcccat cccggtgctc tcgccgcgtg
    40081 gatgggagcg cacgcggtcg tctggaccgt catcgccctc gcgctcgggt ttgtcgactt
    40141 cctcgacagc tggaaggtcg tgctgctggg ggcatggatc ggcgttgccc acgcggtgtt
    40201 gcagtgggcc accacgagcg tgatcgtcgt cgacggggtg ctggtcagcc ggaacttcgg
    40261 gtgggtccag cgggtgccga tcgacgagat cgagctggtg cagcgggtgc gtcaccgccg
    40321 ctggagcggc gtgatgctgc aactccggag cggtcaggag gtgacgctcg cagcgccgct
    40381 gtcgtcggtc ctcgcgccga acccgcactt cgaggaggac ctcaaccgcc tgtgcgcgag
    40441 catcgccttc ggcgaggtca acggcgagtg accggctcgc gccggagcga cgtccgctga
    40501 gcaccggtcg cgtcgaagtt ggtcgggtcg agccaggcaa cgagtgcggc gcggcgacgg
    40561 ggccactcgt cgtcggtgat cgacagccag ttcgtgtccc ggctgcggcc cttgtagacc
    40621 atcgcgtttc ggaacgtgcc ctcgtaggtg aacccgagcc ggtcggctgc cgcgttggac
    40681 ggtccgttga gcgagtcgca cttccactcg tagcggcggt acccggcggc gaaggcggca
    40741 tcggccatga ggaacatcgc ctcggtcgcg gcggtggttc gggcgagggc tggtgagaag
    40801 cagatcgccc cgacctcgat cgagccgatg ggcggcgcga tgcggaggta gctggccatg
    40861 ccgaccgctc ggtcgtcgac gacgatcgcg taggggagct gatcgtcggg ccgccccacg
    40921 gcctcgacga tgcgggtgag ctcgtcgacc gactccgggc gttcgaacgg cagatacgtg
    40981 aagctccggc cgtcgtcggc gcggtactcc tcgaacagcg tcgccgagtg gacctcgggt
    41041 acgtatcgtt cgacccggca cgtgcggccg atcaggggtc ggccgtcggg cgcgggcggc
    41101 ggggtccagt cgccgatcgg cgggccgatc ggattgccga agcggtcggt ccgcggcgtc
    41161 tcgtcggtgg tcatgttcgg cacgataggt gccggtcagc ccctgaggcc tcggagttcg
    41221 ccgaggacgc gggggagctc ggtgagcagt cgatcgatgt cgtcgtcggt cgtcgaccag
    41281 cccaccgaga cgcggagcga gtggtgggcg tcgacgccca tggccgccag caccggcgac
    41341 ggttccagcg cctccgacga gcaggagttg ccggagtgca cggcgacacc ggcgcggtcg
    41401 aggccgatca ggatcggttg tggttcgatg ccggcgatcg ccaggcacac gaggtgcggc
    41461 attcgatgca ccgggtcgcc gagcacgtcg acgcccggtt cgttcgcggc ccacccgatg
    41521 acccgctgcg tgagggcggc cgttcgctcc gcctcggcgt ccagttcgag ggtggcctcg
    41581 tcgagtgcag cggcgaagcc ggccattccg gtgaggttct cgaggccggc ccgacgggct
    41641 cgttcctggt cggcgcccgg gaggagcgga tcgagacgga caccgcggcg tacgagcaac
    41701 gcgccgatgc ccgtcgggcc tccgaacttg tgcgccgtga ccgacatgag gtcgatgccg
    41761 gactcgtcga acgcgatggg cacccggccg atcgcctgcg cggcgtcgac gtgcagcagc
    41821 accccgcggg cgcggcactc ggcggcgacg gttgccaccg gctgcacggt gccgacctcg
    41881 tggttggccc actgcaggtg caccactgcg gtgtcggggc cgatcgctgc gatcacggca
    41941 tcggggtcga cccgaccgcc ccggtccacc ccgaccacgg tcgccggtcc gcgatcggcc
    42001 caggcccgga cggcggagtg ctccactgcg ctcaccaccg tgtgggcggc ggacgggtcg
    42061 ggcccgcgcc gcgccgcacc gtgtgcggcc atggcgatcg cctcggtggc gccggagcag
    42121 aagaccacct cacggggtcg agcgccaagg gcccgggcga cccgctcgcg ggcgtgttcg
    42181 acaccgtgtc gggcgacgag gccctcgtgg tgggagcgac ccgggtcgcc gagctcgccg
    42241 cggctcgact ggcggagcac ctcggccatc gcatcggcag cgaccgggcg cagcggcgcg
    42301 gtcgaggcgt ggtcgaggta ggcacgcgcc atggtcggct agctgtcgat gtcggtgatg
    42361 cagcgggcgt cgcccttggg cagcgacgag gagaggtcga ccggcgtttc gccgtacagc
    42421 gcgccgagca tgcccgagac catgccgcga tcgacggcgc agatgaccgg atgctcgatg
    42481 accgcctcgc cgaacgggca gtgctcgctg acgatgcgga gttggtcgcc gtgttgctcg
    42541 gctcgtgacg cgaacccgtg agccgacagt gcgtcggcga cggcgtggag cgcggtgcga
    42601 accgaacgtt ggccggcggt cgtgtcgccc atcgcgtcgg ccatcgcccg gccgtagtcg
    42661 acccccacct gctcggccag cgccgtcgcc cgctcctcgc ccagctcggc gagtgcacgg
    42721 cccaggaggt tgatgaggat gtcgtcgtgg cggacgggca tctcgaaggg catgtggtcg
    42781 ctgagcaccc ggtaccgctt ggacggccgg cccgctccgc tggcgcggac cgtggcgacc
    42841 tcgaggtacc cgccggcggc gagcttgtcg aggtgatgac gggcgacgtt ggcgtgcagt
    42901 tcgaagcgct gggcgacctc ggacgcggtc acgccgtcgg ccgactcgtg ggcgaagagg
    42961 aagatctcgc ggcgggtcgg gtcgccgaac gcggacgtga ttgccgagac ggccgacgcg
    43021 aagtcggtcg ggttcaagcg gttcgtgatg tccggggtcg gcgccgtggc catgggccca
    43081 ggctacctgg cccctgacgc gtcgccgggt gcagtgcccc gggcgcgggt gacgggtgac
    43141 gagcggcacg acgggcctgt tcgccgctgg cccgccgagt gtcgcagaat gggtgcatgt
    43201 ccttgaacga aaccgctgtc atcgaggccc tccgcccggt acaggacccc gagttgcacc
    43261 gctccatcgt cgacctcggc atggtgcgcg acgtggtggt cgacggcacg aaggttgcac
    43321 tcaccgtggt gctcacgatc cccggctgcc cgctcaaggg cgagatcacc ggccgggtca
    43381 acgaggccct cggcacgatc ggggtcaccg acgtcgatct ggcttggggt gcgatgaacg
    43441 acgaggagcg cgctgccctc cgccagctcc tccacggcga cccggcgtcg acggcggggt
    43501 cgcagcaggc ccacgggcac gccgagggcc gggccatccc gttcgccgac ccgttgtcgc
    43561 gcacccgcgt gctgttgatc gcgtccggca agggcggcgt cggcaagtcg tcggtgacga
    43621 ccaacctggc ggttgcgctg gcccagcgcg gcaagcgcgt cggcatcgtc gacgccgacg
    43681 tctggggctt ctcgatcccc aagatgctcg gggtcgaccg ggcgccggtg ctgctcgacc
    43741 agatgatcgt cccgcccgag gtcaacggcg tgtcgtgcat ctcgatgggg ttcttcgccg
    43801 aggaggacca ggccgtgatc tggcgcggtc cgatgctgca caaggccgtc gagcagttcc
    43861 tcaccgacgt cttctgggac gagcccgact acctgctcgt cgacctgccg cccggcacgg
    43921 gtgacatctc gatctcgttg gcgcagttcc tgccccgcgc cgaggtctac gtcgtcacca
    43981 ccccgcagcc ggccgcgcag cgtgtcgccc agcgcgcggc atcgatggcc aagaaggtca
    44041 acctgccgct ccgtgggatc atcgagaaca tgagctggtt caccggcgac gacggcaagc
    44101 gctacgagct gttcggcgag ggtggcggcc agcgtttggc cgacgacacc ggcgtgccgc
    44161 tccttggcaa ggttccgctg ttgccggctc tccgcgaggg gagcgacacc ggtcatcccg
    44221 tggtcgtcgc gttccccgac accgaggcgg cgcaggcgtt ccgggcgatc gcggagacga
    44281 tcgacgtcga gatggcgccc acccgtcgct tcaaccgcga gctgaagatc ggctgacctc
    44341 ggccggcttc gctcggtgtc gtacccagct gggtacggtc gctgcgctcc ctcctctcgc
    44401 tccgttgcct cggttcgatc cggctgacga ggcgtcctgg atccagcgcc tacgatggat
    44461 ccgttctgcc catctcagag gagtcacccc gtggacgtac ggatcggaat cacccagtcg
    44521 atgcgagagc tcgaagtcga cctcggcgac gaggtcgtgc gggagaccct gaaggccgac
    44581 atcgccgcgg cgatggccaa cgacgacggc atcctgtggc tgaccgacaa gaagggccgc
    44641 gagatcgccg tgcccaccgc caagatctcc ttcatcgaga tcggcagccc cgacgcaccc
    44701 cgcaacatcg ggttctccgt ctga
//
LOCUS       DAOSOA010000003        41462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_997_length_41462_cov_6.688458, whole genome shotgun sequence.
ACCESSION   DAOSOA010000003 DAOSOA010000000
VERSION     DAOSOA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 41462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41462
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_997_length_41462_cov_6.688458"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PKY13_00520"
     CDS             complement(<1..1106)
                     /locus_tag="PKY13_00520"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOT78432.1"
                     /translation="MIRSYAIVLAVTTLVTAALVPLMVRLAGRLGAIAIPNDRKVHAT
                     PTPTLGGGAMFIGMLAGVGVASTLGQFDTMFESRENIIGVVVGAFVIFMTGVIDDIRE
                     VSAPAKVAGIVLAGSILALAGISIVNVPLPIIGFTVLSPDLAALVTVVWVLVMANAVN
                     LIDGLDGLAAGLMAIASGAFLLYGIKLDQSGALFRGNIGPLIAVVTLGVCLGFLVWNF
                     HPAKIFMGDSGALLLGLLMASSTIAVGGQSDDSFTGQSWFFFAPIVIPVLILAVPIGD
                     MIFAILRRTTRRTGFATADKDHLHHRLLRLGHGHRQTVVILWMWTALLSAFALYPAIT
                     GRSTLMIPIAAAAGALGLFTLLAPMVGRRGGERRR"
     gene            complement(1237..2478)
                     /gene="glyA"
                     /locus_tag="PKY13_00525"
     CDS             complement(1237..2478)
                     /gene="glyA"
                     /locus_tag="PKY13_00525"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT78433.1"
                     /translation="MGWPTERDTELFDLIDRETERQNTGLQLIASENFTSPAVMEATG
                     SVLTNKYSEGYPFKRYYGGNEVVDDIETIAIDRVKALFGAEFANVQPHSGANANLGVY
                     LALLEAGDTVLGMSLDHGGHLTHGSPVNISGRFYNFVPYQVTPSDERIDYDQIRDLAL
                     EHRPKLIVAGATAYPRVIDPEVLRSIADDAGSLLMFDAAHIAGLIAGGAHPNPVPYAD
                     IVTFTTHKTLRGPRGGCILAREEYGAAINKAIFPGLQGGPLEHVIAGKAVAFREASHP
                     SFSEYAHQIVANAQALAAGLGSEGFRLVSGGTDNHLMLVDLRPFDEELTGKDAQIVLD
                     EAGITLNKNTVPDDPRSPFVTSGLRIGTPSVTTQGMREAEMAQIAGFIARVLRHRSDA
                     AEVAKVRAEVADLCAKFTPYV"
     gene            complement(2532..2978)
                     /gene="rpiB"
                     /locus_tag="PKY13_00530"
     CDS             complement(2532..2978)
                     /gene="rpiB"
                     /locus_tag="PKY13_00530"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOT78434.1"
                     /translation="MTRFALGSDHAGFELKNVLAEHLRHLGHDVRDLGAHSEDRVDYP
                     DFGAAVGRSVADGESDLGVCVCGSGIGIAIAANKIPGARAATVHDVTSGRLAREHNDA
                     NIICIGERLTGPQVALDTLDAFVAAEFQGGRHTARVAKLDALLPRP"
     gene            3023..4516
                     /locus_tag="PKY13_00535"
     CDS             3023..4516
                     /locus_tag="PKY13_00535"
                     /inference="COORDINATES: protein motif:HMM:NF036848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOT78435.1"
                     /translation="MARPSTSTPAPNTPVIIGVGQFLNRDDDDPIDPVALATVAARLA
                     ADDCGTTAVLQQLDAIAVVPIVSWRYSDPGRLIAEALGARPALTMYPLVGGNTPQMLM
                     NRVCQRIADGELSSALVCGAEAYRTRMRARKADAPLPWARQDLDLQPGWTDGTTYEMG
                     HAAELALGILMPTQAYPLFENALRHESGRSAAEHTAHIAAIWSRYSAVAARNPNAWDR
                     TEYSAEEIATVTPSNRIVGYPYTKHMVSNPDVDMASAVIVCSAEKAAALGVHRDLWVF
                     PHAGADGKDPLMSSRTSFTSSAAIRHGGRAALDLAGVGIDDIDHVDVYSCFPSAVEIG
                     CAELGLSTDRQLTVYGGLCFAGGPWNNPVGHAIAAMVGTLRSDPGSWGLVTANGGNIQ
                     KHSFGVYSTEPPAAGFRTATPQAEIDDEPVVDVLESYEGPATIETWTVMHERDGSVGR
                     AHAVVRVPSGERAWAVSPDADLATALEDDDPIGWSVTIGADAEFTRS"
     gene            4548..4934
                     /locus_tag="PKY13_00540"
     CDS             4548..4934
                     /locus_tag="PKY13_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78436.1"
                     /translation="MLDHNPLLDPIDGSEHQMFGGCQIDVVPAGAGHIKRIIYPPGHR
                     WTTAIKPLVGTEFCQHNHVGFLASGELTFEFPDGCVQAYVAPVAVAVEPGHDAYVVGD
                     EPAVLIQIDFGSDTNTVLGLPETHAH"
     gene            complement(4962..5381)
                     /locus_tag="PKY13_00545"
     CDS             complement(4962..5381)
                     /locus_tag="PKY13_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT78437.1"
                     /translation="MPFWKRDSESVDWLANVAFFEGFDHEHLRRVAALSSEVESPAGS
                     VIIDQGDAGVDCFVIVEGSASVYMSDQFIAAVGDGTMIGEMSLVDHRPRNATVVADTD
                     CKLLRFQAKQFRQLLDEMPKASERVMTLLHDRMRREA"
     gene            complement(5419..6027)
                     /locus_tag="PKY13_00550"
     CDS             complement(5419..6027)
                     /locus_tag="PKY13_00550"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT78438.1"
                     /translation="MSSDVPPPSAASVDEVIAALLAGACVGIPTDTVYGIAARLDDPV
                     AIEALFALKGRAATKAMAVLVDEFETAAELGHFDGVATDLARRFWPGPLTLVVRRREG
                     VVADLGGDHATIGVRCPDHDVVRDLARAVGPIVTTSANRTGEPTIETAADIAAAFGPG
                     LAAVLDGGRLSDAASTVVDVTGTGLVVLREGPISTADLTDLP"
     gene            6130..6939
                     /locus_tag="PKY13_00555"
     CDS             6130..6939
                     /locus_tag="PKY13_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78439.1"
                     /translation="MQLLEKKAKCPLCGAKNPVDAPRCGTCTRPLQNDPLPSQALYDE
                     ALWSTKIASKGARSSTNPYAVLALLVVAAVIVNYFVLGFGPSWAHEAKPPAKGFEWKV
                     YTGQPDYRADLPGEPITGSAGAFGTTLTTATVWVDGHWDLVRDRDTRSVGALDLARRN
                     VHAALILASGPAPADPATALSTLVQTLQPDTQLEPGGVDTVQDPPYGQQITLATSFTG
                     FPEANGSGTVRATAIVLDGKLYIAASFVTGGDDASLHSRLTSHFTPTGAPE"
     gene            complement(6964..7890)
                     /gene="prmC"
                     /locus_tag="PKY13_00560"
     CDS             complement(6964..7890)
                     /gene="prmC"
                     /locus_tag="PKY13_00560"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT78440.1"
                     /translation="MTEPDAASTMAEDDLAGTVTWGELLDEARARFERVGSASAAVDA
                     RRIVEAASGFDGPRFVLARTERVTERAMAAYDLMVARREAGEPLQYVVGSWGFRRLDL
                     AVDRRALIPRPETEMLVDVALSHVDRVLAETGRSPVSVVDLGTGSGAIALSIASERVA
                     TSVWATDASAEALGLARANLAGLGRAATRVRLVEGEWFAALPETLVGSVDVIVSNPPY
                     VTDDDPLPAEVEQWEPAAALRSGPDGLDDLRVIIADAARWLAPGGALVVELDPRQAAT
                     VAELMQQHGFVEATVVADLAGRDRVVAARWKP"
     gene            complement(7883..8950)
                     /gene="prfA"
                     /locus_tag="PKY13_00565"
     CDS             complement(7883..8950)
                     /gene="prfA"
                     /locus_tag="PKY13_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT78441.1"
                     /translation="MLDRLTSLERELRDVDAMLADPDVFSDQSRFVMLSKRRKELEPI
                     VERSQELRQVTEDLETAKEMFAELAGDDRETMRAEVDGAEQRIEELEGEIKVLLIPRD
                     PNDDRNVIIEIRGAEGGEEANLFARDLFEMYRAFAARQGWGFEVMNSSPSDLGGFSDV
                     TFLLKGEGAWSRMKFEAGPHRVQRVPVTESQGRVHTSSATVTVLPEAEEVDVVIDPND
                     LVIDIYRSSGPGGQSVNTTDSAVRITHKPTGIVVSMQDEKSQLQNKTKALVVLRSRIL
                     KAEQDRQAAELSSARKSQVGGGGRSEKIRTYNFKENRVSDHRIGFTAYNLDKVLAGEL
                     DEVSDALVTDERTHQLADLGD"
     gene            complement(8994..9989)
                     /locus_tag="PKY13_00570"
     CDS             complement(8994..9989)
                     /locus_tag="PKY13_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78442.1"
                     /translation="MTALPEERLGQLRVAKLVALSGVDRATASDAGPLGTGAGLIDPS
                     GRAWILIESADRRSLGGAVTWAVRKGASSLVLIVEAGADVAGHLAREATLFALPIEVR
                     RIDGTVAVDAVPVAPPVAPPPLLPTDEIAALLAAADVDVVVEYGVTKAEVLGLEVGRV
                     VEVDGTTRLEVGVGRFDREISAMMFSNVPTAEALAKAVEMVRRYRRPGATTHPLRDLV
                     PERWLRRLVLADPSLVGAAELIAVDTTIEPESLRESQPSAAVGVTAAGTPIVVVCTAG
                     VDLDVVPLAADTRAAVAADAELVICGPARILVSVTRAVAAQLRVPARFVELDLPY"
     gene            complement(10027..10329)
                     /locus_tag="PKY13_00575"
     CDS             complement(10027..10329)
                     /locus_tag="PKY13_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOT78443.1"
                     /translation="MKADIHPNYRYVVFQDTSSGDRILTRSTIASNETVVWDDGNEYP
                     LAKVEISAFTHPFFTGTMKIIDTAGRVERFQKRYGERKRKGPEAAAPVEASESTDE"
     gene            complement(10424..11968)
                     /gene="rho"
                     /locus_tag="PKY13_00580"
     CDS             complement(10424..11968)
                     /gene="rho"
                     /locus_tag="PKY13_00580"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT78444.1"
                     /translation="MAGDSQNMERSTLERKDRAELAAVAAALGLKVPSRAKKADIVTL
                     ILDVVAQPGDSASSAPEAPSSDRATDSPADIAAEATGAAVVEATTDAPIAGQQQLPTV
                     EADHEAADDADESASQSHDEAQDEPARADDASADVSSPSDGGETPEGEGGGRNRRRRR
                     RGRDRAEGDAGAGAGAPGQEFDGEPVEVSGLLDLRDDGYGFLRVAGFLPSRDDVYVSV
                     KQCRQLGLRKGDHLTGLARPAGHKEKNPALTRVETVNGMDPEAARNRPRFDDLTPILP
                     DDELALARESTDGDRTGALIDAIAPIGKGQRALVAAPPRSGRTEVLKQIATSIEANHP
                     DVQLIVLLIDERPEDVTEFRRTIEGGEVVASTFDRPAEEHTAVAELTIEHAKRLVEYG
                     YDVAILLDGITRLAKAYNVTAAPNGHILAGGLDASALYPTKRFFGAARNIEEGGSLTV
                     VATAAIETGSALDAAIYDEFKDTANRELRLTGDSSGALNVALDTSATPAAQQNLLDEL
                     ERRAQG"
     gene            complement(12187..13341)
                     /gene="apgM"
                     /locus_tag="PKY13_00585"
     CDS             complement(12187..13341)
                     /gene="apgM"
                     /locus_tag="PKY13_00585"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOT78445.1"
                     /translation="MKYVVCVPDGCADEPFDELGGRTPLEVAHMPVLAGLAARSEVGR
                     AAVIPEGFAPGSDVGNMSILGYDPARFHTGRAPIEAAALGLKLPADRLAYRTNLVTVG
                     ADGTMVDFAGGHPDNDLAAAIIAAVDAELGTGGDLGLEFHPGVQYRHIMVAPADWLDA
                     DCTPPHDITDQAAVWPTGAAAQRLRDVMEASREIVARFDTKATQIWLWGQGFQPQMTP
                     FAEHYGRRAGMVTAVDLVRGLGVLTDMEIPVVEGANGWYDTNYEGKRDAAIAGLRDGA
                     DVFVIHVEATDEAGHAGNAAEKVTALENWDARILADLVPALDEMGPWRLLMMPDHATP
                     LRTKTHTSDPVPYLLFDSRVDGPGGVYTEAGVADAAVVAGHELMGRLLSV"
     gene            complement(13472..14890)
                     /gene="thrC"
                     /locus_tag="PKY13_00590"
     CDS             complement(13472..14890)
                     /gene="thrC"
                     /locus_tag="PKY13_00590"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOT78446.1"
                     /translation="MKYVSTRGTAPVLEFGDVLLEGLATDGGLYLPESWPPLPDLAAD
                     ADYVTIAVEVMWPFVEGSISRDEFAAMVADAYGTFHPPAGSTPDVVPVVDLRDNLWLA
                     EIFWGPTLAFKDIALQLVGRLFDHELERRHDRVTIVGATSGDTGSAAIDACRDREHID
                     IFILHPEGRTSEVQRRQMTTVPSANVHNIAVDGTFDDCQDLVKALFADEGFRREVQLS
                     AVNSINWARVMAQIVYYVTSARAVNQRLGRPLDAPVNFCVPTGNFGNIFAGFAAMRMG
                     LAIDQLIVASNSNDILTRFFNTGTMDIRGVSPTLSPSMDIQVSSNLERLLFEMSGRDG
                     AGMAELMLRFRSDGSVTVLPDQMAWLSERFSAGRYDDDECLALIGERFRDDGILIDPH
                     SAVGVGVAERLRRGTDVPTICLGTAHPAKFPDAVEQATGQRPPLPDHLADLFERPERF
                     ATMADDVETLKAYVRSHLVSHS"
     gene            complement(14894..16219)
                     /locus_tag="PKY13_00595"
     CDS             complement(14894..16219)
                     /locus_tag="PKY13_00595"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOT78447.1"
                     /translation="MSGAIEIEGAEPPKRVRLGLLGCGNVGAALVGLIERDHDEIAAR
                     TGISLEVTRVAVRSMSKERGLSLPDGVLTTDGASVASDPDVDVVVEVLGGIEPAREYI
                     TAALHAAKPVVTANKELLANVGGELAEVADGAGVDLLFEAAVAGGIPLIRPLRESLVG
                     ERITRVMGIVNGTTNFILSQMSDRGTGYAEALAEAQSLGFAERDPTADVEGYDAGAKA
                     AIIATIAFGARVVAGDVYHEGISNISAADIAYAEQMGYRIKLLAICERFDDDDSVAVR
                     VHPTMVPVGHPLAAVSGSFNAVFVEGVSVGELMFYGRGAGGLPTASAVLGDVIDAAQN
                     LAKSTHASLGSIGRSRIRPIDELSSAFYVNLEVADEPGVLAAVAGVFGGHGVSIRSME
                     QEGLDDQARLVFITHEASESAVRATLKELAALDSVQRVNSVLRVIGREG"
     gene            complement(16271..18001)
                     /locus_tag="PKY13_00600"
     CDS             complement(16271..18001)
                     /locus_tag="PKY13_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOT78448.1"
                     /translation="MASNALGLEDLVAAMPSAAAVINRQGATVVSNPAWSAIAPDLAL
                     LFPSATPDVPDVIELEPTLTLIDHRPSPAETIRAAIRGSLPEAVERLRIRPTAGRSSE
                     PRFVEVRVAEIGDESSFVLATCTEVASLDGGDTGWDLVDRHPLTGLPGRRLFLELLGQ
                     AMTMSDRHRYHPAALAVGISGLDEVEIQHGAEARNELTVAVASRLKAGLRPGDVVAQT
                     GDEQFVIMLPDVDGAYLAEAVAHRLGAEVGRPFRLGNARAAVELSVGVATAESSKSPS
                     TVLAAAEAALVEARLDPAEGVNAIRRVVVAGGLGDASVYEVGSGQRHINVADLREEEL
                     VSYFQPIFDITDRRVVAGEALMRWRHPHYGVLSAGEFLGLAINSGLLATLTDQALVCS
                     TETWADLRDRLGPRPPQLFVNLSPDQLLSRVAVDRLYHLLIATGLPPEDVVVEVTEEA
                     MSTRFGEMLAVLTELRTHGLRVALDDFGSGYSSLGRLRHLPVDVIKVDQSMVRGLETD
                     RRARQLLAAVSTMATELDVECIVEGIETAGEASIVGDLGFRYVQGYHFARPCPAEDFV
                     NLIDPAVQLG"
     gene            complement(18149..19435)
                     /gene="lysA"
                     /locus_tag="PKY13_00605"
     CDS             complement(18149..19435)
                     /gene="lysA"
                     /locus_tag="PKY13_00605"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT78449.1"
                     /translation="MSVLPFHLLPEHATIDAEGELAIGGLRLTDLGAEFGTPLFVYDE
                     AHLRARCREAVAAFGDGVAYASKAFLCRAMARLVHEEGMKIDVASGGELFVALAAGVP
                     ASRCVFHGNNKSVGELTMAIEAGIGTIVVDSFDELDRLDALVGPGGPLDAAAPQRVMI
                     RVTPGIEVHTFDEVQTGQEDSKFGFGWRSGAAKEAAARAQASAWLDLVGLHMHIGSQV
                     FSADNFAHAVEIVAPLARELDLAEFCVGGGLGVAYVEGEEGATISQWGRAVREVCVAA
                     GIAGKVTAEPGRSIVAQAGVTLYTVGTIKQLEGIRTYVSVDGGMSDNPRPVLYGSGYE
                     TFLPRAVGAERTATVRVVGKHCESGDVLVRDGVVPDDLSIGDILCTPVTGAYGHSMGS
                     NYNKVLRPPVVFVNDGVARVVVRGESFDDLVRLDAE"
     gene            complement(19443..21107)
                     /gene="argS"
                     /locus_tag="PKY13_00610"
     CDS             complement(19443..21107)
                     /gene="argS"
                     /locus_tag="PKY13_00610"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT78450.1"
                     /translation="MIRTELADALRAQLAAAGVDVEGDIHLEEPARREHGDWSSNVAM
                     ANAKRLGRNPRELAQQIVDGLNADLPTHVERAEIAGPGFVNFHLRPTWLHAVLRSVVA
                     AGVDRYAQLDVGTGTKVMVEFVSANPTGPLHAGHARGAVYGDALARLLTRAGYEVARE
                     FYINDRGGQMLKMGASMLARATGEPIPEGGYAGNYIAEWATQLPADVVAQGDVEAATA
                     VGYAAALADQREVLGALGVVFDVWFSEMSLVNHGAIEQTLEDLRARGVVDDRDGAVWL
                     RSTDYGDDKDRVLVKSDGSYTYLLPDIAYHRDKFARGFDLLINVWGADHHGYIARMKA
                     AVQALGHEPDQVDIEITQLVKLLKDGQEVKLSKRTGDFIELRDVIDEVGADATRLVYL
                     LQSIDSPQTVDLAVIAAQSMDNPVFYIQMAHARLCSIALRAAERGVTRPALEEVDLGP
                     LVHPRELDVLRLINALPSVIELAARERAPHKVTSWLRELASEIHGFYHDCPILRDDVD
                     DATRFARLWLAEAALVGLRVGLSVLGASAPDSMPSLADATSDVPEA"
     gene            21189..21260
                     /locus_tag="PKY13_00615"
     tRNA            21189..21260
                     /locus_tag="PKY13_00615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21222..21224,aa:Arg,seq:ccg)
     assembly_gap    21267..21366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21450..22685
                     /gene="tyrS"
                     /locus_tag="PKY13_00620"
     CDS             21450..22685
                     /gene="tyrS"
                     /locus_tag="PKY13_00620"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT78451.1"
                     /translation="MTADEPLDPTLAAEIAELTAGCAEVLPADGLASRLAEARAAGRP
                     LRVKLGIDPSGTDLTLGHAVVLRKLRQFQDYGHRAVLIIGDFTGRVGDPTGRSEVRKV
                     QTADDTTANAATYLDQVKRILLDDPELLEVRRNSEWLGTMDFSEVLGYTRLLTVARLL
                     ERDDFSKRYKGGQPISLMEFMYPLMQGLDSVFIDADVELGGTDQTYNNLVGRNLQSQL
                     DRPAQTVMTLPLLRGTDGGDKMGKSLNNFIAISEPAAEQYGKVMSISDDLVGHYAELC
                     CSWNLAEVAALNEQLAADPFAAKHAVARRIVELYWGADAAAEAADKWTRRFSNREIIA
                     EDLPEFAVEAGPLDLIEVLVTSGLVTSKGDARRQLAQGAIKLDGTKLATDADVAHSDD
                     LVGKVLQRGKRHAVRVVAA"
     gene            complement(22701..24095)
                     /locus_tag="PKY13_00625"
     CDS             complement(22701..24095)
                     /locus_tag="PKY13_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT78452.1"
                     /translation="MALIVGAVLAWGVIQFNRIDRAELDLEATASLEPQNFLVVGSDT
                     RDLAGEDGADAEGIFGGDGEKAPGGKRADTIVIARVDPAKATVELLSVPRDLWVTSPS
                     GSEERINSAYNDGPQNLIDTIDRNLGISINHYVEVNFSGFKGLVDSVGGVPLYFDRPV
                     RDSNTGLNIKKAGCYTLDGVQALAFARSRHLVYSDGSKWISDQTGDLGRITRQQVFLR
                     HAMTKVTQLGLDDLDSLRVLTGVAVNNVTIDDQLGTGDMIGLARRFKSFDPSTMVVHR
                     LTTTPFRTKRGADVLQVSKADSQPVLDIFSGKAAASQVTTVPTTALAPGTVTVDVLNT
                     VKIGGLAAKGAADLEAVGFKIGKIGDGTGAKANTVFYATGSKAQADLVASKISPVPAV
                     KEDPTLAGGKVVVKLVGELNVPEAANATPSAGGATNVTTPASTVPTSGAGTVTPEKTL
                     GLNIGDPPAGVSCG"
     gene            complement(24453..25691)
                     /locus_tag="PKY13_00630"
     CDS             complement(24453..25691)
                     /locus_tag="PKY13_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT78453.1"
                     /translation="MDLSYPPEAETFRTEIRAWLEENLPDGWFDEGFEMSADEKKAWN
                     EEWTQKLFDGGWICATWPVEYGGKGLTTIQGVVLSEEFARAKAPMRADFFGDTLVGPT
                     ILMNGTEEQKKYFLPKILNGTMRWCQGFSEPNSGSDLASLKTTAVLDGDEWVITGQKV
                     WTTQAQFADYCFVLARTDPDVKIQAGISYLLVPMQQPGVEVRPITQPDGTAEFNEVFF
                     DGARCPKDNVVGGLNNGWKVANDTLGFERGMSATTGHRRFEEEYKRMVRAAQKNGKID
                     DPLIRQGLATYYTKIQILRINGLRNLTAALTGKRDMGTATLGATNKMFWSEMHKAAME
                     LALDIYGPESMLITTGPDEGWPGVARIGGRDDYPVSPMMTAFFFSRSETIWGGTSQIQ
                     RNIVGEKVLGLPREPKAPTK"
     gene            complement(25781..26716)
                     /locus_tag="PKY13_00635"
     CDS             complement(25781..26716)
                     /locus_tag="PKY13_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78454.1"
                     /translation="MDIVRIARHARAHRGVITQTYLDTIGVNSSRAGHEVRAGRWRRL
                     HEGVFTAAATPDSWELRAEAALAALPAGVLGLRAAARVHHLDLADTTLDVVVPHGGRH
                     RLDGVTVHQSSVPEWHVVRRNGWRVTTLERTLVDLGKVVPANTLQRCIEDQIVDRRTT
                     MARVESVFGELAGRGRPGIARTRSVLGRLDPEPPTESELEAQFIRLLERHGVDLPRRQ
                     VSFDWIQAGRGRVDFWYPERRLIVELDGRRFHLRVAAFEADRRRDLVGLTHGIETVRI
                     THRQLATESAFVAASLRQLLSTEPNSRLTRVHDDG"
     gene            complement(26915..27370)
                     /locus_tag="PKY13_00640"
     CDS             complement(26915..27370)
                     /locus_tag="PKY13_00640"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOT78455.1"
                     /translation="MTEAPMTAPVDPSAERRARYPVGPWTGAAVAACGCVAVALVDPT
                     KHMLTPPCPMRAITGWWCPFCGATRAASRLLRGDVLSALQFNAVFVVLLPLIAVLWTA
                     AAFPGRFSWLDPIRARSTSILTAVAVLFGVFMVVRNTPFADTWLRYPGA"
     gene            27412..28596
                     /locus_tag="PKY13_00645"
     CDS             27412..28596
                     /locus_tag="PKY13_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOT78456.1"
                     /translation="MSTVIETPPPTSGKAERWTNQWLELKAEVIDAKLCTGCAGCVVA
                     CPHDVIGYNHEAGGYTPFHLEEELGPDNCVHGEKGCTSCTRACPRFRLWEHQANEHLF
                     DRDREPDEVAGIYQDIMLTRASDDMVHQMGQDGGLVSAILLWAMEHDYVDAALTSYFE
                     NDDMTTWKAVPGVASTRDEVLKGAGSRYTYSANTLALDEALEQGHKRLAMVGMSCQSS
                     IAPTMWSRKIGKISKPIVFNIGLLCSKTFDDAIFEELFWAKYGLPKAEMTKMNIKGVF
                     QIWMKDGSYHEINLKECHAWTREGCNHCPDFAAEHADISTGGIGKDNDWTLTIVRTDL
                     GREIMQRMIADGSVIARPGDSDPAAIALMRKLAEKSRARWPETAEASVRVGLPIPKRK
                     GA"
     gene            complement(28618..28971)
                     /locus_tag="PKY13_00650"
     CDS             complement(28618..28971)
                     /locus_tag="PKY13_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78457.1"
                     /translation="MAVSVAGCYQNPDPTEWNAKAKSNFIAGCSTDVTAKGGTTTSIA
                     IASSDTCECIYELIKKPESGEQGKWGIDWDDLKDYESKQADAKPADAPPTPPKNLTKA
                     IDECTAVAGPVTSDS"
     gene            complement(29129..29320)
                     /locus_tag="PKY13_00655"
     CDS             complement(29129..29320)
                     /locus_tag="PKY13_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78458.1"
                     /translation="MSPLGILDTDVTVAAVPRFSKSSPALWDAIVDQLAPRDPAGTAP
                     SWPAEPVLPEAGMFDFVRS"
     gene            complement(29374..30084)
                     /locus_tag="PKY13_00660"
     CDS             complement(29374..30084)
                     /locus_tag="PKY13_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT78459.1"
                     /translation="MAPDSLTLVAEQVSQCRRCPRLVAWREEVAATRRAAFRDEEYWG
                     RGVPGFGDPHATLLIVGLAPAAHGANRTGRMFTGDRSGDWLYRALWRAGFANQPESTS
                     ADDGLELTGAFVTSPVKCAPPNNTPTPEERDTCRPFLERELELLTEVRVLVALGAFGF
                     AETARWFGIRPKPKFAHGAEVALPGDRTLIGSYHVSQQNTFTGRLTEDMLDAVFSRAR
                     ELGSPSAVGRNADTSPPT"
     gene            30158..30805
                     /locus_tag="PKY13_00665"
     CDS             30158..30805
                     /locus_tag="PKY13_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HOT78460.1"
                     /translation="MAARPAPPKPTLVVLVRHGQTATTGQLLPGRAPGLHLADAGRAQ
                     ADKAAQRLGAMGGIAAVYASPLERTRETAAPIGKAVGQRVRTNRGLLECEFGDWTGRK
                     LADLNKLPEWKTVQGAPSTFRFPGGESFTEMQTRICSEIDRLVAKHPGERIVCVSHAD
                     PIKAAVAQAQGVHLDLFQRIVISPCSITPILYGAGAPIVLAVNSTGDDLSTLAPS"
     gene            30813..31316
                     /locus_tag="PKY13_00670"
     CDS             30813..31316
                     /locus_tag="PKY13_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HOT78461.1"
                     /translation="MSSSFDLPELRSFAVGTEGPAGERVFYLQAVAEHQVVTLRLEKQ
                     QVALLADYLARVIASYELAESSPTSMPDLYQPVISEWIVGSMMVAIDEAESRVIVIAE
                     ELVIEDDDADPDSPPTGSQARFGLTRAQVDAFVTGARDVIESGRPSCPLCGRPVNADG
                     HFCPRLN"
     gene            31327..32154
                     /locus_tag="PKY13_00675"
     CDS             31327..32154
                     /locus_tag="PKY13_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HOT78462.1"
                     /translation="MNHEPSVDDDFDDDIDGFDDDYDDGAADDRTEISDVLDASVGVT
                     DPDAEALLRAGDIEILGRMPWSSNGTYLVTVTDGDRRCQAIYKPASGERPLWDFPTGL
                     WRREVAAFELARELRWEIVPPTVERDGPLGPGSVQFFVPARFEEHYFTFKDVPEHRRS
                     LRQLCTFDLVSNNTDRKGGHILLSDNGHLWGIDHGLSFHEEFKLRTVMWDYAGEPIET
                     DLIDDVARWLEADPSAALSELLSEAEIAAMRDRANAVIAGGHFPHDPTGRRYPWPLV"
     gene            32426..33511
                     /locus_tag="PKY13_00680"
     CDS             32426..33511
                     /locus_tag="PKY13_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT78463.1"
                     /translation="MSTPPERPERQSRARAGLGVDPTPAADSAPTTRAATTRSAGGAP
                     PVRPTTGPERAVDLDRLRASAASALSAERAPTSDSSRPSASDGRERVAPSTPTSGGAH
                     GWLKIALPVVVVAALATVGVTVLGGGTDGGSAPSASTTEVTSGVTAPPTTAPAAPTTA
                     SSETIVPSTSAAPTTAAAPANPSADPTAPPPHKATYHDGKLYLEGRITSDAEAQKYVE
                     KAAAVLGPDNVVNNYVVDPTVPLSTDGTVYVDEPFLFETGSAELNPQYTGVLGLGVAA
                     LKLNPAARLVVTGYTDNQGDPAKNQQLSLARANAVVEYMVTTGGIDRSRFDAIGAGDQ
                     NPVGDNTTAEGRALNRRIDVKLVNLLG"
     gene            complement(33589..34551)
                     /locus_tag="PKY13_00685"
     CDS             complement(33589..34551)
                     /locus_tag="PKY13_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT78464.1"
                     /translation="MTLSKIWVLAEAIEGEVAPITLEMLTKARELATTVEAVFGGDAS
                     SIAGVLGAHGATAVHATGDLGDSLPGVPVAAAIAAAVGTAAPDAIFLGTTQDGRDIAG
                     RLSVALDVPVITNVTDLVERDGALAGVEPIFGGSQDVYTKFTGSGVGVFLVRPKSFAA
                     EESGGGAASVEALPVPDTGAAGAAKVVNRHVEERTGPQLDAAAVVVAGGRGLGEAANY
                     QLIEDLAKALNAAPGASRAIVDAGWVPYSYQVGQTGKVVKPTVYIAAGISGATQHLVG
                     MKGSKNIIAINKDPEAPIFSVADLGIVGDVHKVLPKLLEALQAR"
     gene            complement(34562..35338)
                     /locus_tag="PKY13_00690"
     CDS             complement(34562..35338)
                     /locus_tag="PKY13_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT78465.1"
                     /translation="MNIVVCVKQIPDPADPGALDPESKTLKRDGKLILDESDSYGVEM
                     ALQLVGDSGEVTLVSMAPNGEVSGLRTALAMGAAKAVLVSDDALKGSDALTTAKVLAA
                     AIGKAGEYDLVLAATESSDGYTGVVPEQIAAVLGLPSVTFAKSVTIDGDTVKVDRQTE
                     AGYDEVVSPLPAVVSVTAGVVEPRYPSFKGIMAAKSKPVDQLTLADIGIEASNAGWAG
                     AGQEIIEVATAAQREAGEIIEDDGEAFTKVVDFLSNLKVI"
     gene            35435..36364
                     /locus_tag="PKY13_00695"
     CDS             35435..36364
                     /locus_tag="PKY13_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOT78466.1"
                     /translation="MKVLLIVNAAASSVTPRRQVAVHRLLSAAHDVRMVETRHRGHAT
                     EFAKEGVDHGVDVVAVFGGDGTLNEAANGLVGSDVALAPLPGGSTNVFARTLGLSDHP
                     VDALRTTLVALSEKSIHRIGVGSVNGRYFLFHTGIGWDARLVRQVEKRAHLKRRLGHG
                     LFVWAGLETWFHLYDRTVPHFRVVYDDGTTTERGCFSIVQNSNPYTYVGKRPFNLSPD
                     VTLDGPLSAMTLTELKSGPFIKLMFETLRSRDAMLRSDIVDYHPDVNSLTVEMLHPVP
                     YQVDGDDLGDAIHLEFRHVPAALDLVVPVGFGT"
     gene            complement(36455..37141)
                     /locus_tag="PKY13_00700"
     CDS             complement(36455..37141)
                     /locus_tag="PKY13_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOT78467.1"
                     /translation="MTVVIGHRGASADFPENTLDAFAGAAAQGADWVELDVRATSDGV
                     LVVHHDAVLPDGRPISDLAHHEVPISVPRLEAAFGVMAGMGVNVEIKNSPAEPGWDPT
                     GELASATVELIAEVAAGLEILVSCFDLATLDHVRAVAPHLATGYLVLSAEEPVDAVAA
                     AVASGCVAVNPWDPCVSSESIARAHDAGLAVNVWTVDDPDRIRQLAEWGADAVITNRP
                     AVALSALGRG"
     gene            37353..37643
                     /locus_tag="PKY13_00705"
     CDS             37353..37643
                     /locus_tag="PKY13_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT78468.1"
                     /translation="MALTSSRSLTLANDDWRSVAACQDTDPDLFFPVGTTGPAIEQID
                     TAKAVCDTCSSKAPCLEFAMITNQDSGVWGGTSEEERRKLRRAWLASQRKSA"
     gene            complement(37815..39293)
                     /locus_tag="PKY13_00710"
     CDS             complement(37815..39293)
                     /locus_tag="PKY13_00710"
                     /inference="COORDINATES: protein motif:HMM:NF023702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT78469.1"
                     /translation="MGPPQTDLLASRSTLDDAALDHLGRLMREWAMLADFCFSDLVLY
                     VLDRDGRYLMAKHIRPATSQTIYQEELVGQVRTPEQRPLIDEAFRTGVIRRGDIDSAW
                     LGERVRVAAIPVRQGGRTIAVLAREFVSAFRHRPGELEQTYERVFERFSSMIVSGAFP
                     FPPEDVGMTTTPRVGDGVIVVGPTGAIEYSSPNATSTLTRSGVEAVIRGMTLAEVGID
                     STAVRVSLAAGRPGVEDIERPDDVTIALRVLPFLESAPDGQRITGAVALVRDISDLRQ
                     RDRLLLSKDATISEIHHRVKNNLQTISSLLRLEGRRLTEPTARAAVEESVRRIHSIAL
                     VHETLSREPGDDVDFREVLKPLVRMVNEGLVSAERPIRFDIDGDGGVVSSEVATSLSL
                     VVTELLQNAVEHAFPDSVEVTERRVGIELRHDDRHIGVVVADNGVGFDPDVSPAPAGS
                     LGQSIVTALVRDELRGTIEYRVGGASTGTRVEVTVPSHKRWE"
     gene            complement(39301..40473)
                     /locus_tag="PKY13_00715"
     CDS             complement(39301..40473)
                     /locus_tag="PKY13_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOT78470.1"
                     /translation="MAAKRVAPAPVTIEQLCAAKEILVSTGSGGVGKTTTAAALGAQA
                     AVTMGAKVLVLTVDPAKRLANAMGLQSFGNVETRVPAEAFAEIGVHPEGELWAAMLDT
                     KQSWDDLIARHAPDTRTRDEILANPLYQNITGKFVQSHDYVAMERLYEIHTSGRYDLI
                     VVDTPPTRNAVDFLDAPERMADFFSSKLLRWIIAPYRSKVVSFASKPFYSVADRILGT
                     QFLQDIAEFFILFQSMYDGFVERADAVQHLLASPSTSFVVVSTLEAAPAREARFFLDL
                     LGERKLHVGALVLNKVLPDYLLDRGAATAARRMVAEADELAAGLTDVWGDLPFGPRIL
                     DEIGTSFVNYQLVAQREAELRSELSVGAGTVATVPYFSEDIGDLAGLLALGERLWR"
     gene            complement(40473..41426)
                     /locus_tag="PKY13_00720"
     CDS             complement(40473..41426)
                     /locus_tag="PKY13_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOT78471.1"
                     /translation="MTTRPHLLDRKLLFVTGKGGVGKSTIASSLALLAANEGKRTLVC
                     EVDAKGNLAEFYGTKPLRFAPTEVQSGLFAMTMDTEESLREYLRLQLHLPLISRLGPL
                     AKTFDFVATAAPGVKEILTVGKLAYEVRERNYDMVVVDASATGHVVGQLASPVAMKEL
                     VSVGIVRQQTEWMLNILTDPAQTGVVVVATPEEMPVNETIELIDRLDSETDINVAAVI
                     ANRVLPELFSRDEEAAFDQLDAEPGRAALAALLGPGSDLVVDAARLAADLRRSRAVHL
                     ANLASRLPDTVPLVNVPYLFTRSHGIRTTRQVADRLAEELL"
BASE COUNT         6584 a        14315 c        14316 g         6147 t
ORIGIN      
        1 cgccgacgct caccgccgcg ccggccgacc atcggagcga gcaacgtgaa gaggccgagc
       61 gcgccggcgg ccgcggcgat cgggatcatc agcgtcgacc gccccgtgat cgccgggtag
      121 agggcgaacg ccgacaggag cgccgtccac atccacagga tcacgacggt ctgtcggtgg
      181 ccgtggccca atcggagcaa ccgatggtgg aggtgatcct tgtcggccgt ggcgaagccc
      241 gtccggcgcg tcgtccgtcg caggatcgcg aagatcatgt cgccgatcgg aacggccaag
      301 atgagcaccg ggatcacgat cggtgcgaag aagaaccacg actggccggt gaacgagtcg
      361 tcgctctgcc cgcccaccgc gatcgtggac gacgccatca gcaggcccaa cagcagcgcg
      421 ccgctgtcgc ccatgaagat cttcgccggg tggaagttcc agacgaggaa cccgagacac
      481 actcccagcg tgaccacagc gatgagcgga ccgatgttgc cgcggaacag cgccccggac
      541 tggtcgagtt tgatgccgta gaggaggaac gctcccgacg cgatcgccat gaggccggcc
      601 gccaggccgt cgagcccgtc gatcaggttc acggcgttcg ccatcaccag cacccacacc
      661 acggtcacga gtgcggccag atcgggagag aggacggtga acccgatgat cggcaggggc
      721 acgttgacga tcgagatgcc ggcgagcgcc aggatcgacc ctgcgagcac gatgccggcg
      781 accttcgcag gcgccgagac ctcccggatg tcgtcgatca ccccggtcat gaagatcacg
      841 aacgcgccga cgacgacacc gatgatgttc tcccgcgact cgaacatcgt gtcgaactgc
      901 ccgagggtcg acgcgacgcc gacaccggcc agcatgccga tgaacatggc accgccgccc
      961 aacgtgggcg tgggcgtggc gtggaccttg cggtcgttcg ggatggcgat ggcgcccaac
     1021 cgcccggcga ggcggaccat gagcggaacc agcgccgccg tcaccaacgt ggtgaccgcg
     1081 agaacgatcg cgtacgaacg gatcacgcga ccaccatatg gccgaggcca cctccgctgc
     1141 gcttcggcca ctcgacccgc ccggcctcac ccgagctcag gttcgctccc gactccgtcg
     1201 ggagctgctg acgctctccg ctcggcggca ctgccatcag acgtagggcg tgaacttcgc
     1261 acacaggtcg gcgacctcgg cacgcacctt ggcgacctcg gcagcgtcgg aacggtgacg
     1321 gaggacccgg gcgatgaagc cggcgatctg cgccatctcc gcctcccgca tgccctgggt
     1381 cgtgaccgag ggcgtgccga tgcggagacc ggaggtcacg aacggcgacc ggggatcgtc
     1441 gggcaccgtg ttcttgttga gggtgatgcc ggcctcgtcg agcacgatct gggcgtcctt
     1501 gcccgtcagt tcctcgtcga aggggcgcag gtcgacgagc atgaggtgat tgtcggtgcc
     1561 gcccgacacc aggcggaagc cttcggagcc gaggccggcg gccagcgcct gggcgttggc
     1621 gacgatctgg tgggcgtact ccgagaacga cgggtgggac gcctcacgga acgccaccgc
     1681 cttgccggcg atcacgtgct cgagcgggcc gccctgcaga cccgggaaga tcgccttgtt
     1741 gatggcggcg ccgtactcct cacgagcgag gatgcacccg ccacgggggc cgcggagcgt
     1801 cttgtgggtg gtgaaggtga cgatgtcggc gtagggcacc gggttggggt gggcgccacc
     1861 ggcgatcagg ccggcgatgt gagcggcgtc gaacatgagg agcgagccgg cgtcgtcggc
     1921 gatggagcgc aggacctcgg ggtcgatgac tcggggatag gcggtcgccc ccgccacgat
     1981 gagcttggga cggtgctcga gggccaggtc ccggatctgg tcgtagtcga tgcgctcgtc
     2041 cgacggtgtc acctggtagg gcacgaagtt gtagaagcgg cccgagatgt tcacggggct
     2101 gccgtgggtg aggtggccgc cgtggtcgag gctcatgccc agcaccgtgt cgcccgcctc
     2161 gagcagcgcc aggtacacgc cgaggttggc gttggcgccg gagtgcggct gcacgttggc
     2221 gaactcggca ccgaagagcg ccttgacccg gtcgatggcg atggtctcga tgtcgtcgac
     2281 gacctcgttg ccgccgtagt accgcttgaa gggatagccc tcggagtact tgttggtgag
     2341 caccgagccg gtggcctcca tcacggcggg cgacgtgaag ttctccgagg caatgagctg
     2401 cagcccggtg ttctgtcgct cggtctcacg gtcgatgagg tcgaagagtt cggtgtcgcg
     2461 ttcggtgggc catcccatgg cggggccttt cggtggtgga ggagcgggtc ggtgggcggg
     2521 acgcagcggc gtcaggggcg cgggagcagg gcgtcgagct tggcgacgcg ggcggtgtgg
     2581 cggccccctt ggaactcggc cgcgacgaac gcgtcgagcg tgtcgagggc gacctggggg
     2641 ccggtgaggc gctcaccgat gcagatgatg ttggcgtcgt tgtgctcgcg ggcgaggcgc
     2701 ccggaggtga catcgtggac ggtggccgca cgggcgcccg ggatcttgtt ggccgcgatc
     2761 gcgatgccga tgccgctacc gcagacgcac acacccaggt cggactcgcc gtcggcgacg
     2821 gaacggccca cggcggcacc gaaatcgggg tagtcgacgc ggtcctcact gtgggcaccg
     2881 aggtcccgca cgtcgtggcc gaggtggcgg aggtgctcgg ccagcacgtt cttgagctcg
     2941 aagccggcgt ggtcggaacc gagggcgaag cgggtcatgg ccggattgta gttgcgtccg
     3001 aggacctacc tattctcggc ggatggcccg cccctccacc tcgacgccgg ccccgaacac
     3061 tccggtgatc atcggcgtcg ggcagttcct caaccgcgat gacgacgacc cgatcgatcc
     3121 cgtcgccctc gccaccgtcg ccgcccgcct ggccgccgac gactgcggca ccaccgcggt
     3181 gctgcagcag ctcgacgcca tcgccgtggt gccgatcgtg tcgtggcgct actccgatcc
     3241 cggccgcttg atcgccgaag cgctcggggc ccgcccggcg ctgaccatgt atcccctggt
     3301 cgggggcaac acaccgcaga tgctgatgaa ccgggtgtgc cagcgcattg ccgacggcga
     3361 actgtcatcg gctctcgtgt gcggcgccga ggcgtaccgg acccgcatgc gcgcccgcaa
     3421 ggccgatgcc ccgctcccgt gggcgcgaca ggacctcgat ctccaacccg gctggaccga
     3481 cggcacgacc tacgagatgg gccacgccgc tgagctcgcg ctgggcatcc tcatgccgac
     3541 ccaggcgtac ccgctgttcg agaacgcgtt gcgccacgag tccggccgga gcgcggcgga
     3601 acacaccgcc cacatcgccg ccatctggtc ccgctacagc gcggtggctg cccgcaaccc
     3661 caacgcctgg gaccgtacgg agtacagcgc cgaggagatc gccacggtca ctccgtccaa
     3721 ccgcatcgtc ggctacccgt acaccaagca catggtgtcg aaccccgacg tcgacatggc
     3781 gtcggctgtc atcgtctgct ccgccgagaa ggcagcggcg ctcggagtgc accgcgacct
     3841 gtgggtgttc ccccacgccg gcgccgacgg caaggatccg ctcatgtcca gccggacgtc
     3901 gttcacgtcg tcggcagcga tccgccacgg cggccgggca gcgctcgacc tcgccggagt
     3961 gggcatcgac gacatcgacc acgtcgacgt gtactcctgt ttcccgtcgg cggtcgagat
     4021 cggatgtgcc gaactcggcc tgtcgaccga tcgccagctc acggtgtacg gcggtctgtg
     4081 cttcgcgggc ggcccgtgga acaaccccgt cggacacgcg atcgccgcga tggtcggcac
     4141 gctgcgctcg gaccccggct cgtggggcct cgtgaccgcc aacggcggca acatccagaa
     4201 gcacagcttc ggcgtgtact cgaccgaacc gcccgccgcc gggttccgca ccgccacgcc
     4261 gcaggccgag atcgacgacg agcccgttgt cgacgtgctc gagtcctacg agggtcccgc
     4321 cacgatcgag acctggaccg tgatgcacga gcgggacggg tcggtcgggc gtgcccatgc
     4381 cgtggtgcgc gtgccgagtg gcgaacgggc gtgggccgtc agccccgatg ccgacctcgc
     4441 caccgcgctc gaggacgacg acccgatcgg ttggtcggtg accatcggcg ccgacgccga
     4501 gttcactcgc tcctgaccgg ggccacccga cggcgtaacg tgcgggcatg ctcgaccaca
     4561 acccgctcct cgaccccatc gacggctccg aacaccagat gttcggcggc tgccagatcg
     4621 acgtcgtgcc cgccggcgcc ggccacatca agcggatcat ctacccaccc ggccatcgtt
     4681 ggaccacggc gatcaagccg ctcgtcggta ccgagttctg ccagcacaac cacgtcgggt
     4741 tcctcgccag cggcgagctg accttcgagt tccccgacgg ctgcgtccag gcgtacgtcg
     4801 ctccggtcgc cgttgccgtc gagccgggcc acgacgccta cgtcgtcggc gacgagcccg
     4861 cggtgctcat ccagatcgac ttcggcagcg acaccaacac cgtgctgggt ctgccggaga
     4921 cccacgccca ctgaccagcg ggcgttgatg cctcgcgagg ctcaggcctc gcggcgcatc
     4981 cggtcgtgca acagcgtcat gacgcgctcg gacgccttgg gcatctcgtc gagcagctga
     5041 cgaaactgct tggcctggaa ccgcaggagc ttgcagtcgg tgtcggccac gacggtggcg
     5101 ttgcgcggcc ggtgatcgac gagtgacatc tccccgatca tcgtgccgtc gccgacggcg
     5161 gcgatgaact ggtcgctcat gtagaccgac gccgacccct cgacgatgac gaaacagtcc
     5221 acaccggcat caccctggtc gatgatgacc gagccggcgg gcgactcgac ctcggaggac
     5281 aacgctgcga cacgacgcag gtgttcgtgg tcgaacccct cgaagaaggc cacgttggcc
     5341 aaccagtcga cactctcgga atctcgcttc cagaacggca cgagtgatcc cccttcggct
     5401 ctcgtccgga cgacgatcct agggcaggtc cgtcaggtcg gccgtggaaa tcggcccctc
     5461 gcggaggacg accaggcccg tgccggtgac gtcgaccacc gtcgaggccg catcggagag
     5521 ccgccccccg tcgagcaccg ccgcgagccc gggcccgaac gccgcagcga tgtccgccgc
     5581 cgtctcgatc gtcggctcac cggtgcggtt ggcgctggtg gtcacgatcg ggccgacggc
     5641 gcgggcgagg tcccgaacga cgtcgtgatc agggcagcgc acgccgatcg tggcatggtc
     5701 gccaccgagg tcggcgacca cgccctcccg gcggcggacc accaacgtca gcggccccgg
     5761 ccagaaccgt cgggcgaggt ccgtcgccac cccgtcgaag tgtccgagct cggcggccgt
     5821 ctcgaactcg tcgacgagca cggccatggc cttggtcgcg gcccggccct tgagcgcgaa
     5881 gagtgcctcg atcgcgaccg ggtcgtcgag tcgtgctgcg atgccgtaga cggtgtcggt
     5941 cgggatcccg acgcaggcac cggcgagcag ggcggcaatg acctcgtcga cggatgcggc
     6001 gctcgggggc gggacgtcgg agctcacggc cgcgaccgta ccggctcgac caccgtcgac
     6061 gggccacgcc ctcaactcac ccccggttcg agccgatagg tcatcacaca ctgcgcgcga
     6121 ggacatggga tgcagctact cgagaagaag gccaagtgcc cgctttgcgg ggcgaagaac
     6181 cccgtggacg cgccacggtg cggaacctgc acccggccac tccagaacga tccgctgccg
     6241 tcacaggcgc tgtacgacga ggccttgtgg tccaccaaga tcgcctcgaa gggcgctcga
     6301 tcatcgacca atccctacgc cgtcctggcg ctgctcgtcg tcgccgccgt catcgtgaac
     6361 tacttcgtcc tcggcttcgg cccgtcgtgg gcgcacgagg ccaaaccccc ggccaagggg
     6421 ttcgagtgga aggtctacac gggtcaaccc gactaccggg ccgacctccc cggcgagccg
     6481 atcaccggct cggccggcgc cttcggcacc accttgacta ccgccaccgt gtgggtcgac
     6541 ggccactggg atctcgtgcg tgaccgggac acccgttcgg tcggagcact cgacctggcc
     6601 cgccgcaacg tccacgccgc cctgatcctc gcgtcgggtc ccgcgcccgc cgatccggcc
     6661 accgcgttgt cgacgctggt gcaaaccctc cagcccgaca cccagctcga gcccggcggc
     6721 gtcgacacgg tgcaggatcc cccgtacggg cagcagatca cgttggcgac gtcgttcacg
     6781 ggattccccg aagcgaacgg ctccggaacg gtgcgggcca ccgccatcgt gctcgacggc
     6841 aagctctaca tcgcggcgtc cttcgtgacg ggcggcgacg acgccagcct gcactcccga
     6901 ctcacgtcgc acttcacgcc gacgggcgcc cccgagtagg tcgctcggtt ccgcctcggc
     6961 ggctcagggt ttccatcgag ccgcgacgac tcgatcacga ccggcgagat ctgccacgac
     7021 cgtcgcctcg acgaagccgt gctgctgcat caattcggcg acggtggcgg cctgacgggg
     7081 gtccagctcg acgacgagcg ccccgccggg cgccagccag cgcgcggcgt cggcgatgat
     7141 cacccggaga tcgtccaggc catcggggcc cgaccggagc gcagcggccg gctcccactg
     7201 ttccacctcg gcaggcagcg ggtcgtcgtc ggtgacgtag gggggattcg acacgatcac
     7261 gtcgacggat ccgaccaacg tctcgggaag ggcggcgaac cactccccct cgacgagccg
     7321 gacccgggtg gcggcacggc cgaggccggc gaggttcgcc cgggcgaggc cgagtgcctc
     7381 ggccgatgca tcggtggccc agaccgaggt ggcgacgcgc tcggacgcga tcgacaaggc
     7441 gatcgctccc gagccggtgc cgaggtcgac gacggacacg ggcgaccgcc cggtctcggc
     7501 gagcacacga tcgacgtggg agagcgccac gtcgaccagc atctcggtct cgggacgcgg
     7561 gatcagggcg cgacggtcga cggcgagatc gagccggcgg aagccccaac tccccacgac
     7621 gtactgcagc ggttcacccg cctcgcgccg ggcgaccatc aagtcatatg ccgccatggc
     7681 tcgttcggtg acccgttcgg tccgggcgag gacgaaccgg ggcccgtcga accccgacgc
     7741 ggcttcgacg attcggcgtg cgtccacggc ggcggacgcg gaaccgacac gttcgaagcg
     7801 ggcccgggcc tcgtcgagca gctcgcccca ggtgaccgtg ccggccaggt cgtcctcggc
     7861 catcgtcgaa gcggcgtcgg gctcagtcac cgagatcggc gagttgatgg gtccgctcgt
     7921 cggtcaccag cgcgtcactc acctcgtcga gctcccccgc cagcaccttg tcgaggttgt
     7981 aggcggtgaa cccgatgcgg tgatccgaca cccggttctc cttgaagttg taggtgcgga
     8041 tcttctcgct gcgaccaccg ccaccgacct ggctcttccg agcgctcgac agctcggccg
     8101 cctgacggtc ctgttcggcc ttgaggatgc ggctccgcag cacgaccagc gccttggtct
     8161 tgttctggag ctggctcttc tcgtcctgca tcgacacgac gatgccggtc ggcttgtggg
     8221 tgatgcggac ggcggagtcg gtcgtgttca ccgactggcc gccggggccc gacgatcggt
     8281 agatgtcgat caccaggtcg ttggggtcga tcacgacgtc gacctcctcg gcctcgggca
     8341 gcaccgtcac ggttgcggac gacgtgtgca cccggccctg ggactcggtg accggcaccc
     8401 gttggacacg gtgcggcccg gcctcgaact tcatccgaga ccacgcgccc tcacccttga
     8461 ggaggaacgt gacatcgctg aagccgccga gatccgacgg tgagctgttc atcacctcga
     8521 acccccagcc ctggcgggcg gcgaacgctc ggtacatctc gaacaggtcg cgggcgaaga
     8581 ggttggcctc ctcgccgccc tcggcgccgc gaatctcgat gatgacgttg cggtcgtcgt
     8641 tggggtcgcg gggaatgagc aggaccttga tctcgccctc gagctcctcg atgcgctgct
     8701 cggcgccgtc gacctcggcc cgcatggtct cgcggtcgtc gcccgcgagc tcggcgaaca
     8761 tctccttggc cgtctcgagg tcctcggtca cctggcgaag ctcctgcgac cgttcgacga
     8821 tcggttcgag ctccttgcga cgcttcgaca gcatcacgaa ccgcgactgg tcggagaaca
     8881 cgtcgggatc ggcgagcatg gcgtcgacgt cgcgcaactc acgttcgaga gaggtcagtc
     8941 ggtcaagcac ggagaccaat tgtactgatc cccaccgagc ctgttttgtg gcatcagtag
     9001 ggcaaatcga gttcgacgaa gcgggcgggg actcggagct gtgcagctac cgctcgggtc
     9061 accgacacca ggatccgagc cggcccgcag atgaccagct cggcatcggc agcgactgcg
     9121 gcgcgggtgt ccgccgccag gggcacgacg tcgagatcga ctcccgcggt gcacacgacc
     9181 acgatcgggg tccccgccgc ggtcacaccc accgccgccg acggctgcga ctcgcgcagg
     9241 ctctcgggct cgatcgtcgt gtcgaccgcg atcagctcgg cggcaccgac cagcgacggg
     9301 tcggcgagga cgagccggcg cagccatcgt tcgggcacga ggtcgcgaag cggatgtgtg
     9361 gtggcaccgg ggcggcggta gcggcgcacc atctcgaccg ccttggcgag cgcctcggcc
     9421 gtcgggacgt tcgagaacat catcgccgag atctcccggt cgaagcgtcc gaccccgacc
     9481 tcgagccgcg tcgtgccgtc gacctcgacg acccggccga cttcgagccc gagcacctcg
     9541 gccttggtga ccccgtactc gaccacgacg tcgacgtcgg ccgctgcgag cagcgcggcg
     9601 atctcgtcgg tcgggagcag cggcggcggt gcgaccggtg gggcgacagg gacggcgtcg
     9661 acggcgaccg tgccgtcgat gcggcgcacc tcgatgggca gcgcgaacag cgtcgcctca
     9721 cgagcgaggt gaccggcgac atcggcaccg gcctcgacga tcagcacgag cgacgacgcg
     9781 cccttccgga cggcccacgt gaccgcgccg cccagggacc gacggtcggc cgattcgatc
     9841 aggatccacg cccggccgct cgggtcgatg agccctgcgc cggtgccgag ggggccggcg
     9901 tcgctcgccg tcgcccggtc gaccccgctc agggcgacca gcttggcgac gcgcaactgc
     9961 ccgaggcgct cctcgggcag tgcagtcatt cgaactccgg acgttggggg taccgaagta
    10021 cccggctcac tcgtcggtgg actcggacgc ctcgacggga gcagcggcct cggggccctt
    10081 gcgcttgcgc tcgccgtaac gcttctggaa acgctcgacg cggccggcgg tgtcgatgat
    10141 cttcatcgtg ccggtgaaga acgggtgcgt gaaggccgag atctcgacct tggcgagcgg
    10201 gtactcgttg ccgtcgtccc acacgaccgt ctcgttcgac gcgatcgtcg accgggtcag
    10261 gatgcgatcg cccgacgacg tgtcctggaa cacgacgtag cggtagttgg ggtggatgtc
    10321 tgccttcata gtggtgccag cctagggaat cggggtgaac gccgccaacc aagcgtggtg
    10381 gcgacgggac ggacggtggg tggaacggga cggggaccgc aggtcagccc tgcgcgcgcc
    10441 gctcgagctc gtcgagcagg ttctgctggg cggccggcgt tgcggacgtg tcgagcgcga
    10501 cgttgagtgc acccgacgag tcgccggtga gccggagctc gcggttggcg gtgtccttga
    10561 actcgtcgta gatcgccgcg tcgagcgccg agccggtctc gatggccgcg gtggcgacga
    10621 cggtgagcga gccaccctct tcgatgttgc gagcggcccc gaagaaccgc ttcgtggggt
    10681 agagcgcgct ggcgtcgaga ccgccggcga ggatgtgacc gttgggagcg gcggtcacgt
    10741 tgtaggcctt ggccaggcgg gtgatgccgt cgagcaggat cgccacgtcg tagccgtact
    10801 ccaccagacg cttggcgtgc tcgatggtga gctcggcgac cgcggtgtgt tcctcggcgg
    10861 gccggtcgaa cgtcgaggcg acgacttcgc cgccctcgat cgtgcgacgg aactcggtca
    10921 cgtcctcggg acgctcgtcg atgagcagca cgatcaactg cacatcggga tggttggcct
    10981 cgatcgaggt ggcgatctgc ttgaggacct ccgtgcgacc cgaccgcggc ggggcggcca
    11041 cgagggcgcg ttgacccttg ccgatcgggg cgatggcgtc gatgagcgct ccggtgcgat
    11101 cgccgtcggt cgactcacgg gcgagggcca gctcgtcgtc gggaaggatc ggcgtgaggt
    11161 cgtcgaagcg gggacggttg cgcgccgcct cgggatccat gccgttcacg gtctcgacgc
    11221 gggtcagcgc cgggttcttc tccttgtgac cggcgggccg ggcgaggccc gtgaggtggt
    11281 cgcccttgcg gagaccgagc tgacggcact gcttgaccga cacgtagacg tcgtcgcggc
    11341 tcggcaggaa cccggcgacc cggaggaagc cgtacccgtc gtcgcggagg tcgagcaaac
    11401 ccgacacctc gacgggctcg ccgtcgaact cctggcccgg tgcgccggcg ccggcaccgg
    11461 cgtcgccctc ggcacgatcg cggccccgcc ggcggcgacg acgattgcga ccgccgccct
    11521 cgccctcggg ggtctcaccg ccgtcggacg gcgacgacac gtcggccgac gcgtcgtcgg
    11581 cacgtgccgg ctcgtcctgc gcctcgtcgt gggactggct cgccgactcg tcggcatcgt
    11641 cggcggcctc gtgatcggcc tcgacggtcg gcagctgctg ctggccggcg atcggggcat
    11701 cggtggtggc ctcgacgact gcggcgccgg tcgcctcggc ggcgatgtcg gcgggcgagt
    11761 cggtcgcgcg gtccgacgag ggagcctcgg gggccgacga cgccgaatcg cccggctggg
    11821 cgacgacgtc gaggatcagg gtgacgatgt cggccttctt ggcccgcgac ggcaccttga
    11881 gccccaaggc tgctgcgacg gcggccagct cggcgcggtc cttgcgttcg agcgtggagc
    11941 gctccatgtt ctgggaatca ccagccattt ggtgagtacc tctttcggga aaatgtggcc
    12001 ggacacagga ggtccgacat aggtgaacgg ccacggacgc gttgcgtccg tgccgggaag
    12061 atcgaccagc gagacggatc gggaaggatc cggaccggac gcgtttgcca cgccgttccg
    12121 ctgtgtgccc gcatcgtatc ggcgttcacc gctcccccac aaccaccgcc gggagcccgg
    12181 cgacgttcac accgacaaca ggcgacccat caactcgtgg ccggcgacga ccgcggcgtc
    12241 ggcgacaccc gcctcggtgt agaccccgcc gggcccgtcg acccgggaat cgaacagcag
    12301 gtacggcacg ggatccgacg tgtgggtctt ggtgcgaagg ggcgtcgcgt ggtcgggcat
    12361 catcagcaga cgccaggggc ccatctcgtc gagcgcaggg acgagatcgg cgaggatccg
    12421 cgcatcccag ttctcgaggg ccgtgacctt ctccgcggcg ttgccggcgt gaccggcctc
    12481 gtcggtcgcc tccacgtgga tcacgaacac gtcggcgccg tcgcggaggc cggcgatcgc
    12541 ggcgtcgcgc ttgccctcgt agttggtgtc gtaccagccg ttggcaccct cgacgacggg
    12601 aatctccatg tcggtgagca caccgaggcc acgcacgagg tcgacggcgg tgaccatgcc
    12661 ggcgcggcgg ccgtagtgct cggcgaacgg cgtcatctgc ggctggaagc cctgacccca
    12721 cagccagatc tgcgtggcct tcgtgtcgaa ccgggccacg atctcgcggc tcgcctccat
    12781 cacgtcacgc agccgttgtg cggcggcacc cgtcggccat acggccgcct gatcggtgat
    12841 gtcgtggggc ggggtgcagt cggcatcgag ccagtcggcg ggggcgacca tgatgtggcg
    12901 gtactgcacg ccggggtgga actcgaggcc caggtcacca ccggtgccga gctcggcgtc
    12961 gaccgccgcg atgatggccg cggcgaggtc gttgtcggga tgtccaccgg cgaagtcgac
    13021 catggtgccg tcggcaccga cggtcacgag gttcgttcgg tacgcgaggc ggtcggccgg
    13081 gagcttcaat ccgagtgccg ccgcctcgat cggcgcacga ccggtgtgga agcgggcggg
    13141 gtcgtagccg aggatcgaca tgttgccgac gtcgctcccg ggggcgaacc cttcggggat
    13201 gacggcggcg cggcccacct cgctgcgggc ggcgagcccg gcgagcaccg gcatgtgggc
    13261 gacctccaga ggtgtgcgac cacccagttc gtcgaagggt tcgtcggcac agccgtcggg
    13321 gacacacacc acgtacttca tggtccggga gggtagttgc cggtagcgga acgctcgaac
    13381 catcccggcc gacgccatca cgccgaggta ccgaggcgaa agaagctccc gaggaacggg
    13441 gagcgaacct gagctgaggt ggccgaggcg atcaggaatg ggagacgagg tgggagcgga
    13501 cgtaggcctt gagcgtctcg acgtcgtcgg ccatggtcgc gaagcgctcg ggtcgctcga
    13561 agaggtccgc gaggtggtcc ggcaacggtg gccgctgccc cgtcgcctgc tcgacggcgt
    13621 cggggaactt ggccgggtgg gcggtgccga ggcagatggt cggaacgtcg gtgccacgcc
    13681 gcagccgttc ggcgacgccg acgccgacgg cgctgtgggg gtcgatgagg atgccgtcgt
    13741 cgcggaaccg ttcgccgatg agcgccaggc actcgtcgtc gtcgtaccgg ccggcggaga
    13801 accgctcgct gagccacgcc atctggtcgg gaagaacggt caccgagccg tcggaccgga
    13861 accgcagcat gagctcggcc atcccggcgc catcgcgacc cgacatctcg aacagcaggc
    13921 gctcgaggtt ggacgacacc tggatgtcca tcgacggact gagggtggga ctcacgcccc
    13981 ggatgtccat cgtgcccgtg ttgaagaagc gggtgaggat gtcgttgctg ttggacgcca
    14041 cgatcagctg gtcgatcgcg agccccatcc gcatggcggc gaaccccgcg aagatgttgc
    14101 cgaaattgcc ggtcggaacg cagaagttca ccggagcgtc gagcggccga cccaaccgct
    14161 ggttcacggc ccgggcgctc gtcacgtagt agacgatctg ggccatgacc cgggcccagt
    14221 tgatggagtt gaccgccgac agctgcacct cacgacggaa gccttcgtcg gcgaacagcg
    14281 ccttcacgag gtcctgacag tcgtcgaacg ttccgtcgac ggcgatgttg tggacgttgg
    14341 ccgacgggac cgtcgtcatc tggcggcgtt gcacctccga cgtccgcccc tccgggtgga
    14401 ggatgaagat gtcgatgtgc tcacggtcgc ggcaggcatc gatggcggcc gagccggtgt
    14461 cgcccgaggt ggcgccgacg atcgtcacac gatcgtggcg acgctcgagt tcgtggtcga
    14521 acagtcgccc gacgagctgg agcgcgatgt ccttgaacgc gagcgtggga ccccagaaga
    14581 tctcggcgag ccagaggttg tcgcggaggt cgacgacggg gacgacgtcg ggggtcgatc
    14641 ctgcgggcgg atggaacgtg ccgtaggcgt cggccaccat cgctgcgaac tcgtcgcgcg
    14701 aaatcgaacc ctcgacgaac ggccacatga cctcgacggc gatcgtgacg tagtcggcgt
    14761 cggcggcgag gtcgggcagc ggtggccacg actcggggag atagagccca ccgtcggtgg
    14821 cgaggccttc caacaggacg tcgccgaact ccaggaccgg cgccgtcccg cgcgtgctga
    14881 cgtacttcac cgttcagccc tcccggccga tgacgcgcag gaccgagttg acccgctgca
    14941 ccgagtccag cgccgccagc tccttgaggg tcgcccggac ggcggactcc gacgcctcgt
    15001 gagtgatgaa cacgaggcgc gcctggtcgt cgaggccttc ctgttccatg gagcggatcg
    15061 acacaccgtg gccgccgaac actcccgcca ccgcggcgag cacacccggc tcgtcagcga
    15121 cctcgaggtt gacgtagaac gccgacgaca gctcgtcgat ggggcggata cgactgcggc
    15181 cgatggagcc gagggaggca tgggtgctct tggccaggtt ctgggcggcg tcgatgacgt
    15241 cgccgagcac ggcgctggcc gtcggcaggc cgccggcgcc tcggccgtag aacatcagct
    15301 cgccgaccga cacgccctcg acgaacacgg cgttgaacga accggacacc gcggccaggg
    15361 ggtgaccgac gggcaccatc gtcgggtgca cgcgaacggc aaccgaatcg tcgtcgtcga
    15421 accgctcgca gatcgcgagc agcttgatgc ggtaccccat ctgttcggcg tacgcgatgt
    15481 cggcggcgct gatgttcgag atgccctcgt ggtacacgtc gccggccacg acccgggcac
    15541 cgaacgcgat cgtggcgatg atcgccgcct tggcgccggc gtcgtacccc tcgacgtccg
    15601 cggtgggatc gcgttcggcg aacccgaggc tctgcgcctc ggccagcgcc tcggcgtagc
    15661 cggtgccgcg gtcggacatc tgggacagga tgaagttggt cgtcccgttc acgatgccca
    15721 tcacccgcgt gatgcgctcg cccaccagcg actcccgaag ggggcggatg agggggatgc
    15781 ccccggcgac cgccgcctcg aacagcaggt cgacaccggc gccgtcggcg acctcggcga
    15841 gctcgccgcc gacgttcgcc agcagttcct tgttggccgt gacgaccggc ttggcggcat
    15901 ggagcgcggc ggtgatgtac tcccgcgccg gctcgatgcc accgagcacc tcgaccacga
    15961 cgtcgacatc gggatccgac gccaccgaag cgccgtcggt cgtgagcaca ccgtcgggca
    16021 acgagagacc ccgctccttc gacatcgacc gcacggcgac gcgggtcacc tcgagcgaga
    16081 tgccggttcg ggccgcgatc tcgtcgtggt cccgttcgat gagccctacc aacgccgcgc
    16141 cgacgttgcc gcagccgagc agcccgagcc gcacccgctt cggcggttca gcgccttcga
    16201 tctcgatcgc ccctgacatg accgcccacg ctaccgggcc gcggtcgtcg accgcgacgg
    16261 ggttacggtc tcagcccagc tgaacggccg gatcgatgag gttcacgaag tcctcggccg
    16321 ggcagggccg ggcgaagtgg tagccctgca cgtagcggaa gccgaggtcg cccacgatgg
    16381 atgcctcccc cgccgtctcg atgccttcga cgatgcactc gacgtcgagc tcggtcgcca
    16441 tggtgctcac ggcggcaagg agctgccggg cgcgacggtc ggtctcgagg ccacgcacca
    16501 tcgactggtc gaccttgatc acgtcgacgg gcaggtgtcg caggcgaccg agggacgagt
    16561 agccgctccc gaagtcgtcg agcgccaccc gcaacccgtg ggtgcgcagc tcggtcagga
    16621 cggccagcat ctcgccgaac cgcgtcgaca tggcctcctc ggtgacctcg accaccacgt
    16681 cctcgggcgg cagcccggtt gcgatgagga ggtggtacag acgatcgacg gcgacccggc
    16741 tgagcagctg gtcggggctg aggttcacga acagctgggg cgggcgcggg cccaggcggt
    16801 cccgaagatc ggcccacgtc tcggtcgagc acacgagcgc ctgatcggtg agcgtcgcca
    16861 gcaggccact gttgatggcg aggcccagga actcccccgc cgacagcacg ccgtagtgag
    16921 ggtggcgcca gcgcatcagg gcttcaccgg cgacgacacg gcgatcggtg atgtcgaaga
    16981 tcggttggaa gtagctgacg agctcttcct cacggaggtc ggccacattg atgtggcgtt
    17041 gcccgctccc cacctcgtac accgaggcgt cgccgagacc gccggccacg accacccgtc
    17101 ggatcgcgtt cactccctcg gcggggtcga gacgggcctc gaccagcgcg gcctcggcgg
    17161 ccgccagcac ggtcgacggg ctcttcgacg actcggcggt ggcgacgccg accgagagct
    17221 cgaccgcggc tcgggcgttg cccaggcgga agggccgacc gacctcggca ccgagacggt
    17281 gggcgacggc ctccgccagg taggccccgt cgacgtcggg gagcatgatc acgaactgct
    17341 cgtcaccggt ctgggcgacc acgtcgccgg gacgcaggcc ggccttgagc cgggaggcga
    17401 ccgcgacggt gagctcgttg cgggcctcgg cgccgtgctg gatctccacc tcgtcgagac
    17461 cactgatgcc gaccgccaag gcggcggggt gatagcggtg acgatccgac atcgtcatgg
    17521 cctggcccag gagttcgagg aagagccgac ggcccggcag gcccgtgagc ggatggcggt
    17581 cgacgaggtc ccagccggtg tcgccgccgt cgagcgacgc cacctcggtg cacgtggcca
    17641 ggacgaacga cgactcgtcg ccgatctccg ccacgcggac ctcgacgaag cgaggctccg
    17701 acgaccggcc ggcggtcgga cggatgcgca ggcgctcgac cgcctcgggc aacgagccgc
    17761 ggatcgcagc ccggatcgtc tcggccggcg agggacggtg atcgatgagc gtgagggtcg
    17821 gctccagctc gatcacgtcg gggacgtcgg gcgtggcgga cgggaacaac agcgccaggt
    17881 cgggcgcgat cgccgaccac gccgggttcg aaacgaccgt cgcgccctgg cggttgatga
    17941 ccgctgctgc gctcggcatg gcagccacga ggtcctcgag acccaaggcg ttcgatgcca
    18001 cgttcgttcc tccgatagtc gagtccggac cggcgcccgg acggtcaccc gccgtcgtcg
    18061 gcggctccgc caccgacccg acgagactag gcacggtcgc ccacccgagg ggggatggcc
    18121 ccggcccgag gagcgacgac ccctgccgtc actcagcgtc gagtcggacc agatcgtcga
    18181 agctctcgcc ccggacgaca acacgagcga cgccgtcgtt cacgaagacg accggcgggc
    18241 ggaggacctt gttgtagttg gaccccatcg agtgcccgta ggcgccggtg acgggagtgc
    18301 acaggatgtc gccgatcgac aggtcgtcgg gcacgacacc gtcgcggacc aacacatcgc
    18361 ccgactcgca gtgcttgccg acgacgcgga ccgtcgcggt ccgctcggcg ccgacggcac
    18421 gaggcaggaa cgtctcgtag cccgagccgt acaacaccgg tcgcgggttg tcgctcatgc
    18481 cgccgtcgac cgacacgtag gtgcggatgc cctcgagctg cttgatcgtg ccgacggtgt
    18541 acagcgtgac gcccgcctgc gcgacgatcg accgaccggg ctcagcggtc accttgccgg
    18601 cgatgccggc ggcgacgcac acctcgcgca cggcacggcc ccactggctg atcgtcgcac
    18661 cttcctcgcc ctcgacgtag gccaccccga ggccaccccc gacgcagaac tccgcgagat
    18721 cgagttcgcg cgcgagcggc gcgacgatct cgacggcgtg ggcgaagttg tcggccgaga
    18781 acacctgcga cccgatgtgc atgtggagcc cgacgaggtc gagccaggca ctcgcctgcg
    18841 cccgcgccgc cgcctccttg gccgctccgg atcgccagcc gaacccgaac ttcgagtcct
    18901 cctggccggt ctgcacctcg tcgaacgtgt ggacctcgat gccgggagtg acgcggatca
    18961 tcacccgctg cggcgcagcc gcgtcgagtg gccctcctgg gcccacgagg gcgtcgagcc
    19021 ggtcgagctc gtcgaagctg tccacgacga tcgtgccgat gcccgcctcg atcgccatcg
    19081 tcagctcgcc gaccgacttg ttgttgccgt ggaagacgca ccgcgaggcc gggacaccgg
    19141 ccgccagcgc aacgaagagc tcgccgcccg acgcaacgtc gatcttcatg ccctcctcgt
    19201 gcaccaggcg cgccatggcc cggcacagga acgccttcga tgcataggcg acgccgtcgc
    19261 cgaacgcggc gaccgcctcg cgacaccggg cgcgaaggtg ggcctcgtcg tagacgaaca
    19321 gcggggtacc gaactcggca ccgagatcgg tcagtcgcag gccaccgatc gccaactcac
    19381 cttcggcgtc gatcgtggcg tgctcgggaa gcaggtggaa cgggagaacc gacacgggcc
    19441 gctcaggcct cgggaacgtc ggacgtcgcg tcggcgagcg acggcatgga gtcgggcgcc
    19501 gaggcaccca gcaccgacaa cccgacacga aggcccacga gcgccgcctc ggccagccac
    19561 agccgggcga accgggtggc gtcgtcgacg tcgtcgcgga ggatgggaca gtcgtgatag
    19621 aagccgtgaa tctccgacgc cagctcgcgg agccagctcg tgaccttgtg cggcgcacgc
    19681 tcccgcgccg ccaactcgat gacggacggc agggcattga tgagccgcag cacgtcgagc
    19741 tcgcgaggat ggacgagcgg gccgaggtcg acctcctcga gcgccggacg cgtcaccccc
    19801 cgctcggccg cccgcagggc gatcgagcac agccgggcgt gcgccatctg gatgtagaag
    19861 accgggttgt ccatcgactg cgccgcgatc accgcgagat cgaccgtctg cggtgagtcg
    19921 atcgactgca gcaggtagac gaggcgggtc gcatcggcgc cgacctcgtc gatgacgtcg
    19981 cgcagctcga tgaagtcgcc ggtgcgcttg gagagcttca cctcctggcc gtccttgagc
    20041 agtttcacca gctgggtgat ctcgatgtcg acctgatcgg gctcgtggcc gagtgcctgc
    20101 accgctgcct tcatgcgggc gatgtagccg tgatggtcgg caccccacac gttgatgagg
    20161 aggtcgaagc cacgggcgaa cttgtcgcgg tgataggcga tgtcgggaag gaggtaggtg
    20221 taggagccgt cgctcttgac cagcacccgg tccttgtcgt cgccgtagtc ggtcgagcgg
    20281 agccacacgg cgccgtcgcg gtcgtcgacc acacctcggg cgcggaggtc ttcgagggtc
    20341 tgctcgatgg cgccgtgatt gacgagcgac atctcgctga accacacgtc gaacaccaca
    20401 ccgagcgctc cgagcacctc gcgctggtcg gcaagcgccg ccgcgtagcc gaccgccgtc
    20461 gccgcctcga cgtcgccctg cgcgacgacg tcggctggca gctgggtcgc ccactcggcg
    20521 atgtagttgc cggcgtaccc accctcggga atgggctcgc cggttgcccg ggcgagcatc
    20581 gacgcgccca tcttgagcat ctggccgccc cggtcgttga tgtagaactc gcgggccacc
    20641 tcgtagccgg cgcgggtgag cagacgcgcc aacgcgtcgc cgtagaccgc gccgcgggcg
    20701 tggcccgcgt gcagcgggcc ggtcgggttg gccgacacga actcgaccat caccttggtg
    20761 cccgtgccga cgtcgagctg ggcgtaccgg tcgacgccgg cagcgacgac cgagcgcagt
    20821 acagcgtgca gccaggtggg acggaggtgg aagttcacga acccggggcc ggcgatctcg
    20881 gcccgctcga cgtgggtcgg caggtccgcg ttgagcccgt cgacgatctg ttgcgcgagc
    20941 tcacggggat tgcggcccaa gcgcttggcg ttggccatcg cgacgttgga ggaccagtcg
    21001 ccgtgctcgc gtcgcgccgg ctcctcgagg tggatgtcgc cctcgacatc gaccccggca
    21061 gcggcgagtt gggccctcaa ggcatcggcg agttcagtgc ggatcacggc gacagcgtac
    21121 cgatcagccg tcgccgctcg gaacgagtaa ccacagggtc gcggccacgg ctatcctcgt
    21181 agcgccccgc cctcgtagct caggggatag agcatcgccc tccggaggcg tgtgcgcgcg
    21241 ttcgaatcgc gccgagggca cctggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnncgac aggcttcgct cccgatccgg gtgtcagggg ttcggccgga gtcggtggga
    21421 agtcgcccga tgagtacgat cgtcccgcta tgactgccga cgaaccgctc gaccccaccc
    21481 tcgccgccga gatcgcagag ctgaccgccg ggtgcgccga ggtgctgccc gccgacgggc
    21541 tcgccagccg cctcgccgag gcccgggcgg ccggacgacc gcttcgggtc aagctcggca
    21601 tcgacccgtc gggcaccgat ctcaccctcg gccacgcggt cgtgctgcgg aagctgcgtc
    21661 agttccagga ctacggacat cgggccgtcc tgatcatcgg cgacttcacc ggccgcgtcg
    21721 gcgacccgac cgggcggagc gaggtccgca aggtccagac cgccgacgac acgaccgcca
    21781 acgctgcgac ctacctcgac caggtcaagc gcatcctcct cgacgatccc gaactgctcg
    21841 aggtccgtcg caactccgaa tggctgggca ccatggactt ctccgaggtg ctcggataca
    21901 cccgcctcct caccgtcgcc cgcctcctcg aacgcgacga tttctcgaag cggtacaagg
    21961 gtggtcaacc catctcgctc atggagttca tgtacccgct gatgcaaggg ctcgactcgg
    22021 tcttcatcga cgccgacgtc gagctgggcg gcaccgatca gacctacaac aacctcgtcg
    22081 gccgcaacct gcagtcccag ctcgaccggc cggcgcagac ggtcatgacg ctgccgctcc
    22141 tccgcggcac cgacggcggc gacaagatgg gcaagtcgct caacaacttc atcgccatca
    22201 gcgagccggc ggcggagcag tacggcaagg tcatgtcgat ctccgacgat ctcgtcgggc
    22261 actacgccga gctgtgctgc agctggaacc tggccgaggt cgcggcgctg aacgaacagc
    22321 tcgccgccga ccccttcgcc gccaaacacg ccgtcgcccg acgcatcgtc gagctctact
    22381 ggggcgccga tgccgcagcc gaggcggccg acaagtggac gcgacgcttc agcaatcgcg
    22441 agatcatcgc cgaggacctg cccgagttcg ccgtcgaggc cggcccgctc gatctcatcg
    22501 aggtgctggt cacgtcgggg ctcgtcacgt ccaagggcga cgcccgtcgc cagcttgccc
    22561 aaggggcgat caagctcgac ggcaccaagc tcgccaccga tgccgacgtg gcgcactccg
    22621 acgatctcgt cggcaaggtc ctgcagcgtg gcaagcgcca cgccgttcgg gtcgtcgctg
    22681 cctgatcgag ctccgggcgc tcagccgcag ctcacaccgg cgggcggatc cccgatgttc
    22741 agccccaagg tcttctccgg ggtgacggtg cccgcgcccg acgtcggcac ggtcgaggcc
    22801 ggcgtcgtca cgttggtggc acccccggcg gacggcgttg cgttggccgc ctcgggaacg
    22861 ttcagctcgc cgacgagctt caccacgacc ttgccgccgg ccagtgtcgg gtcttccttg
    22921 accgccggca cgggcgagat cttcgacgcc accaggtcgg cctgcgcctt cgagccggtc
    22981 gcatagaaca cggtgttggc cttggcgccg gtgccgtcgc cgatcttgcc gatcttgaac
    23041 ccgacggcct cgagatcggc ggcgcccttg gccgccaggc caccgatctt gacggtgttc
    23101 aggacgtcga ccgtcacggt ccccggggcc agggccgtcg tcggcaccgt cgtcacctgc
    23161 gaggccgccg ccttgccgga gaagatgtcg agcaccggct gcgaatcggc cttggagacc
    23221 tggaggacgt ccgccccccg cttcgtgcgg aacggggtgg tcgtcagtcg gtgcacgacc
    23281 atggtcgacg ggtcgaagga cttgaaccga cgggccagac cgatcatgtc gccggtgccg
    23341 agctggtcgt cgatcgtcac gttgttgacg gcgacgccgg tgagcacccg gagcgagtcg
    23401 aggtcgtcga ggccgagctg ggtgaccttg gtcatggcgt gccggaggaa cacctgttga
    23461 cgggtgatgc gaccgaggtc accggtctga tcgctgatcc acttgctgcc gtcggagtag
    23521 acgaggtggc gggatcgggc gaaggccagc gcctgcacgc cgtccagggt gtagcagccg
    23581 gccttcttga tgttgagacc ggtgttgctg tcgcgcacgg gtcggtcgaa gtagagcggc
    23641 acaccgccga ccgagtcaac gagtcccttg aagccgctga agttcacctc gacgtagtgg
    23701 ttgatcgaga taccgaggtt gcggtcgatc gtgtcgatga gattctgggg gccgtcgttg
    23761 taggcgctgt tgatgcgctc ctcgctgccc gacggtgagg tgacccacag gtcacgcggc
    23821 accgacaaca gctcgacggt cgccttcgcc gggtcgacgc gggcgatgac gatcgtgtcg
    23881 gcgcgcttgc cacccggcgc cttctcaccg tcgccaccga agatgccctc ggcgtcggcg
    23941 ccgtcctcgc cggccagatc ccgggtgtcg cttccgacca ccaggaagtt ctgcggttcg
    24001 agcgacgccg tcgcctcgag gtcgagctcg gcgcggtcga tgcggttgaa ctggatcacg
    24061 ccccacgcga ggacggctcc gacgatgagg gccatcaccg cggcaaagac ggcgaaccac
    24121 cggcggtact tgtgcgcctg cgatcggggg cggttggacc ctgcggggcg cggcgccggg
    24181 cgacgagggc cacctcctcc gccaccaccg ccggacacct ggcgggcacg cggagccggc
    24241 cgatcacccc gctgttgggg cggacgacga gccggttggc gagccggttc gcggggctcg
    24301 ggaggacgac gcgccggctg ccggggcgag gacgaactcg agcgcacgcg gcgatcgacg
    24361 ttgtgaggcg gcaccggcac ggagcggaag cgtaatccca ctatcgggcg cgacaggtga
    24421 caccacccac ccgcgccgtg tccgggggcg aactacttgg tcggcgcctt cggttcgcgg
    24481 gggagcccga gcaccttctc gccgacgatg ttgcgctgga tctggctggt tccaccccag
    24541 atcgtctcgg accggctgaa gaagaaggcg gtcatcatcg ggctgaccgg gtagtcgtcg
    24601 cggccaccga tccgggccac accgggccac ccctcgtcgg ggccggtcgt gatcagcatc
    24661 gactcgggac cgtagatgtc gagcgccagc tccatcgccg ccttgtgcat ctccgaccag
    24721 aacatcttgt tggtggcgcc cagcgtcgcc gtgcccatgt cgcgcttgcc cgtcagcgcg
    24781 gcagtgaggt tgcgcaggcc gttgatgcgg aggatctgga tcttcgtgta gtacgtcgcg
    24841 aggccctggc ggatgagcgg atcgtcgatc ttgccgttct tctgggcggc gcggaccatg
    24901 cgcttgtact cctcctcgaa gcggcggtgg ccggtcgtgg ccgacatgcc ccgctcgaac
    24961 ccgagggtgt cgttcgcgac cttccacccg ttgttcaggc cgccgacgac gttgtccttg
    25021 gggcaacggg cgccgtcgaa gaacacctcg ttgaactcgg ccgtgccgtc gggctgggtg
    25081 atcggtcgga cctcgacgcc gggctgctgc atcgggacca gcaggtacga gattccggcc
    25141 tggatcttca cgtccggatc ggtgcgggcc agcacgaagc agtagtcggc gaactgggcc
    25201 tgggtggtcc acaccttctg gccggtgatc acccactcgt cgccgtcgag caccgcggtg
    25261 gtcttgagcg atgccaggtc ggagccggag ttcggctcgg agaagccctg gcaccagcgc
    25321 atggtgccgt tgaggatctt cgggaggaag tacttcttct gctcctccgt gccgttcatg
    25381 aggatcgtcg ggccgacgag cgtgtcaccg aagaagtcgg cgcgcatggg ggccttggcc
    25441 cgggcgaact cctcggagag caccacgccc tggatggtcg tgaggccctt gccgccgtac
    25501 tccaccggcc acgtcgcaca gatccagccg ccgtcgaaca gcttctgggt ccactcctcg
    25561 ttccacgcct tcttctcgtc ggcgctcatc tcgaagccct cgtcgaacca cccgtcgggc
    25621 aggttctcct cgagccacgc acggatctcg gtgcggaacg tctcggcctc gggtgggtag
    25681 ctcaggtcca tccccgcatt cttcaccgat cggtcaggtt cgtccactca gcgatcgttc
    25741 acccgttctc gatcgccgcg gctcggcccg aacttcagcc tcacccgtca tcatggacac
    25801 gggtgagcct cgagttcggt tcggtggaga gcaactgtcg aagcgacgct gcgacgaacg
    25861 ccgactcggt cgcgagctgg cggtgcgtga tccggacggt ctcgatgccg tgggtgaggc
    25921 cgacgagatc ccgacgacgg tcggcttcga acgctgcgac acggaggtgg aaccgtcgac
    25981 cgtcgagctc gacgatcagc cgccgctccg gataccagaa gtcgacccga cctcgaccgg
    26041 cctggatcca gtcgaacgac acctgccgtc ggggaagatc gaccccgtgc cgctcgagga
    26101 ggcggatgaa ctgcgcttcg agctcggact cggtcggtgg ttccggatcc agacgaccga
    26161 gcaccgagcg tgttcgggcg atccccggcc ggccgcgccc tgcgagctcg ccgaacaccg
    26221 attcgacgcg ggccatcgtc gtgcggcgat caacgatctg gtcctcgatg caccgctgca
    26281 gggtgttcgc cggcaccacc ttgccgagat cgacgagcgt gcgttcgagc gtggtcactc
    26341 gccagccgtt gcgacgcacg acgtgccact cgggcaccga gctctgatgg acggtgacgc
    26401 catcgagtcg atgccgacca ccgtgcggaa ccacgacgtc gagcgtcgtg tcggcgaggt
    26461 cgagatggtg aacgcgggcg gcggcgcgaa gacccagcac accagcagga agtgcggcga
    26521 gcgccgcctc ggctcgcagc tcccaggagt cgggcgtggc ggcggcggtg aacacgccct
    26581 cgtggagccg gcgccagcga ccggcccgaa cctcgtgtcc ggcacgggac gagttcacgc
    26641 cgatcgtgtc gaggtaggtc tgcgtgatga caccacggtg agcgcgggcg tggcgggcga
    26701 tgcggacgat gtccaccgat tgctcctgga tcgaatcgag acaggagggg aaggcgatgc
    26761 aacctacgac gcacccccga cactcggcgc gcgccggcgt cgcggtggac gtactcgagc
    26821 ctcacccgtg cccatgatca cgagtgaggc tgaagttcgg acgaggcgag gacggtagcg
    26881 ggcgttggtg cccggctgag cctggattcc gcggtcaggc gccggggtac cgcagccagg
    26941 tgtcggcgaa cggcgtgttg cggacgacca tgaacacccc gaacaggacc gcgacggccg
    27001 tgaggatcga tgtcgagcgg gcgcggatcg ggtcgagcca cgagaaccga ccggggaacg
    27061 cggcggccgt ccacaggacc gcgatgaggg gaaggagcac cacgaacacg gcgttgaact
    27121 ggagcgccga gagcacgtca ccacgcagga gacgtgacgc cgcccgggtc gcaccgcaga
    27181 acggacacca ccagcctgtg atcgcacgca tcgggcacgg cggggtgagc atgtgcttcg
    27241 tcgggtcgac gagggcgacg gcgacgcatc cgcacgccgc gactgctgcg cccgtccagg
    27301 ggccgaccgg ataccgggcg cgacgctcag ccgacgggtc gacgggagcg gtcatcggtg
    27361 cctcggtcat tcgcttcgag gctaaccccc gacgagcggt atcctcagcc catgtcgacc
    27421 gtgatcgaga cacccccgcc caccagcggc aaggccgagc gctggaccaa ccagtggctg
    27481 gagctcaagg cggaggtcat cgacgccaaa ctctgcaccg gctgcgccgg ctgcgtcgtc
    27541 gcctgtcccc acgacgtcat cggctacaac cacgaggccg gcggctacac gccgttccac
    27601 ctcgaggagg agctgggccc cgacaactgc gtgcacggcg agaaggggtg caccagctgc
    27661 acccgcgcct gtccccggtt ccgcttgtgg gagcaccagg ccaacgagca cctgttcgac
    27721 cgtgaccgtg aacccgacga ggtcgccggg atctaccagg acatcatgct gacccgggcc
    27781 tccgacgaca tggtccatca gatgggccag gacggcggcc tcgtgtcggc gatcctgctg
    27841 tgggcgatgg agcacgacta cgtcgacgcc gcgctcacct cctacttcga gaacgacgac
    27901 atgacgacgt ggaaggccgt acccggcgtg gcgtcgaccc gggacgaggt gctcaaggga
    27961 gccgggagcc gctacacgta ctcggccaac acgctggcgc tcgacgaggc cctcgaacag
    28021 ggccacaagc ggctggcgat ggtcggtatg agctgccagt cctcgatcgc tcccaccatg
    28081 tggtcacgca agatcggcaa gatctccaag ccgatcgtgt tcaacatcgg tctgctctgc
    28141 tcgaagacgt tcgacgatgc gatcttcgag gaactgttct gggccaagta cggcctgccc
    28201 aaggccgaga tgaccaagat gaacatcaag ggcgtcttcc agatctggat gaaggacggc
    28261 agctaccacg agatcaatct caaggagtgc cacgcctgga cccgcgaggg gtgcaaccac
    28321 tgccccgatt tcgccgccga gcacgccgac atctcgacgg gtggcatcgg caaggacaac
    28381 gactggacgc tcacgatcgt tcgcaccgac ctcggccggg agatcatgca gcgcatgatc
    28441 gccgacgggt cggtgatcgc ccgacccggt gacagcgacc cggccgcgat cgcgctgatg
    28501 cgcaagctgg ccgagaagtc gcgggcccgg tggcccgaga ccgccgaggc atcggtgcgc
    28561 gtcggtctcc cgatcccgaa gcgtaagggc gcgtaggccc cgaccgtcag tccgggctca
    28621 ggagtccgac gtcaccgggc cggcgaccgc ggtgcactcg tcgatggcct tggtgaggtt
    28681 cttgggcggc gtgggcggcg cgtcggcggg cttggcatcg gcctgcttgg actcgtagtc
    28741 cttgaggtcg tcccagtcga tgccccactt gccctgctca cccgactcgg gcttcttgat
    28801 gagttcgtag atgcactcgc acgtgtcgct cgaagcaatc gcgatgctgg tggtggtgcc
    28861 gcccttggcc gtgacgtcgg tggagcaacc ggcgatgaag ttggacttcg ccttggcgtt
    28921 ccactcggtg ggatcggggt tctggtagca cccggccacg gacaccgcca tcacagcgag
    28981 gccgagggca gccaggccgc ggcgtcgggt ctcggatcgg gatgacttgc caacacgcac
    29041 agggggttct ccgtcgtcga acgtcgggca aaagctaccc gtccggcgtc gacgagacga
    29101 accggggccg cctgggccgc tccgtcgcct acgagcgcac gaagtcgaac atgcccgcct
    29161 cgggcagcac cggctcggcg ggccacgacg gggccgtccc cgccggatcc cgaggtgcga
    29221 gctgatcgac gatggcgtcc cacagagccg gcgagctctt cgagaacctc gggacggcgg
    29281 cgacggtgac gtcggtgtcg aggattccaa gcggggacat gttccagttg tcggcgcgag
    29341 gcgcgccggg caggaggggc cattgggacc atttcaggtc gggggcgacg tgtcggcgtt
    29401 tcgtcccacg gccgatggac tcccgagttc gcgggcacgc gagaagacgg cgtcgagcat
    29461 gtcctcggtg agacgcccgg tgaacgtgtt ctgctggctc acgtggtagc tgccgatcag
    29521 cgtccggtct ccgggcagcg ccacctcggc gccgtgggcg aacttcggct tcggccggat
    29581 gccgaaccac cgtgccgtct cggcgaagcc gaatgcgccg agggccacca ggacccggac
    29641 ctcggtcagc agctcgagct cgcgctcgag aaatggtcgg cacgtgtcgc gctcctcggg
    29701 cgtcggcgtg ttgttcggtg gcgcgcactt cacgggcgac gtcacgaagg ctccggtgag
    29761 ctccagaccg tcgtcggccg aggtcgactc gggctggttg gcgaagccgg cgcgccacag
    29821 cgcccggtag agccagtcgc ccgaacggtc gccggtgaac atccgcccgg tccggttcgc
    29881 cccgtgcgcc gccggagcga gtccgacgat gagcagcgtc gcgtgagggt caccgaagcc
    29941 cgggacacct cggccccagt actcctcgtc acggaacgcg gcccgacgag tggcagcgac
    30001 ttcctcccgc catgccacca ggcgagggca gcgacggcac tgcgacacct gctcggcaac
    30061 cagcgtgagg gaatcgggcg ccacgccgtc actctggcac aggagggtgc agggggatgg
    30121 ggaacaaact tggccgccga gtaggttgac cggtcgaatg gccgcacgcc ccgctccccc
    30181 caaacccacg ctcgtcgtac tcgtccgcca cggacagacg gcgaccaccg gccaactcct
    30241 ccccggccgc gcccccggtc tccacctggc cgacgccgga cgcgcccagg ccgacaaggc
    30301 ggcccaacga ctcggagcca tgggaggcat cgcggcggtc tacgcctcac cgctcgaacg
    30361 cacccgtgag accgccgcac cgatcggcaa ggcggtgggc cagcgggtcc ggacgaaccg
    30421 cggcctgctc gagtgcgagt tcggcgactg gacgggacgc aaactcgcgg acctcaacaa
    30481 gttgcccgag tggaagaccg tccagggggc gccctccacg ttccgtttcc ccggcggcga
    30541 gtcgttcacc gagatgcaga cccgcatctg cagtgagatc gaccggctcg tggcgaagca
    30601 ccccggcgaa cgcatcgtgt gcgtctccca cgccgacccg atcaaggccg ccgtcgccca
    30661 ggcgcagggc gtgcacctcg acctgttcca gcgcatcgtg atctcaccct gctcgatcac
    30721 cccgatcctc tacggcgccg gcgcccccat cgtgttggcg gtcaactcga ccggcgacga
    30781 cctgtcgacg ctggcgccct cctgaggtcc tgatgtcgag ctccttcgac ctccccgaac
    30841 tgcggtcgtt cgccgtgggc accgagggcc ctgccggcga gcgggtgttc tacctgcagg
    30901 ccgtggcaga gcaccaggtg gtcacgctgc gcctcgagaa gcagcaggtc gcgctgctgg
    30961 ccgactacct ggcgcgggtc atcgcgagct acgagctcgc ggagtcctcg ccgacgtcca
    31021 tgcccgacct ctatcaaccg gtcatctcgg agtggatcgt cggttcgatg atggtcgcca
    31081 tcgacgaggc ggaatcacgg gtgatcgtga tcgccgagga actggtcatc gaggacgacg
    31141 acgccgatcc cgacagccct ccgaccggct cccaggcgcg gttcggtctg acccgggcgc
    31201 aggtcgacgc gttcgtgacc ggcgcacgcg acgtcatcga gtcgggccgc ccgtcatgcc
    31261 cgctgtgcgg tcgaccggtc aacgccgacg gccacttctg cccccgtctc aactagccgc
    31321 cacgtcatga accacgagcc cagcgtcgac gacgacttcg atgacgacat cgacggcttc
    31381 gacgacgact acgacgacgg tgccgccgac gaccgcaccg agatcagcga tgtcctcgat
    31441 gccagcgtcg gagtgaccga ccccgacgcc gaagcgctgt tgcgggcggg cgacatcgag
    31501 atcctgggcc gcatgccgtg gagctcgaac ggcacgtacc tcgtgaccgt caccgacggc
    31561 gaccggcgct gtcaggcgat ctacaaaccc gcgtccggag aacggcccct ctgggacttc
    31621 ccgaccggtc tctggcggcg cgaggtcgcc gcgttcgagc tggcccgcga gctgcgctgg
    31681 gagatcgtcc cgcccacggt cgagcgcgac ggacccctcg gccccggctc ggtgcagttc
    31741 ttcgtgccgg cccggttcga ggagcactac ttcacgttca aggatgttcc cgagcaccgg
    31801 cgctccctcc ggcagctgtg caccttcgac ctggtgtcga acaacacgga ccgcaagggc
    31861 ggtcacatcc tgttgagcga caacggacac ctgtggggca tcgaccacgg cctgtcgttc
    31921 cacgaggagt tcaagctccg aacagtcatg tgggactacg ccggagaacc gatcgagacc
    31981 gatctgatcg acgacgtcgc acgctggctg gaggccgacc cgtccgccgc gctgtcggag
    32041 ctgctgagtg aggccgagat cgcagcgatg cgcgaccgcg ccaacgccgt gatcgccggt
    32101 ggacacttcc cccacgaccc cacgggccgc cgatacccct ggcccttggt ctgagcggtg
    32161 tccggacccg gacccatcac tccctccagc gacgaatccg agtccgaaga cctcgagcca
    32221 agtgcatcgc cagcgagggg aggttgctgg aaacgatgcc gctgaaccga ggttcagcga
    32281 gccctgcaag caggatatgg gcgctgcgag ctgccccaaa tgagcaattc tccctctccc
    32341 tcatcgggcc gtacgactgc ccgcggctgc tcaagcgggc tgctcgcgaa cccgatcacc
    32401 tcaaggtgaa catcggagat cagacatgag cactcccccg gaacgtcccg aacgccagag
    32461 ccgggctcgc gccggactcg gcgttgaccc gacccccgcc gccgactccg ccccgacgac
    32521 gcgggcggcg acgacacgat ccgccggcgg cgctccgccc gtgcgaccca cgaccggccc
    32581 cgagcgcgcc gtcgacctcg accgtctccg cgccagcgcg gcatcggcgc tgtccgccga
    32641 acgagcaccc acctccgact cctcgcgtcc gtcggcatcg gacgggcgcg agcgggttgc
    32701 cccgtcgaca ccgacgagcg gcggagcgca cgggtggctg aagatcgcgc tccccgtcgt
    32761 tgtggtcgcc gcgctcgcca ccgttggtgt caccgtgctc ggcggcggca ctgacggcgg
    32821 atccgctccg tcggcgtcga cgacggaggt cacctccgga gtgacggcac ccccgacgac
    32881 ggcccctgcc gcaccgacga cggcctcctc cgagacgatc gtcccgtcga cgtccgcggc
    32941 gccgaccacc gccgccgcgc cggcgaatcc gtccgccgat ccgacggcac cgccgcctca
    33001 caaggccacc taccacgacg gcaagctcta cctcgaaggc cggatcacct ccgacgcgga
    33061 ggcccagaag tacgtcgaga aggccgccgc cgtgctcggt ccggacaacg tcgtcaacaa
    33121 ctacgtcgtc gacccgaccg tacccctgtc gaccgacggc acggtgtacg tcgacgagcc
    33181 gttcctcttc gaaaccgggt cggccgagct caacccgcag tacaccggcg tgctcggcct
    33241 cggcgttgct gcgctgaagc tcaaccccgc ggcgcgcctg gtcgtcacgg gttacaccga
    33301 caaccagggt gaccccgcca agaaccagca gctgtcgctc gcccgcgcca acgccgtcgt
    33361 cgagtacatg gtgacgaccg gcggcatcga ccgaagccgc ttcgacgcca tcggggccgg
    33421 cgaccagaat cccgtcggcg acaacaccac cgccgagggc cgagccctga accgccgcat
    33481 cgacgtcaag ctggtgaacc tcctcgggtg aaccgccctg gccctcgggg tgaacgcgaa
    33541 cgacccccgc cgtgaggcgg gggtcgttgg cgttcgtgcc gaatcggctc agcgggcctg
    33601 cagggcctcg aggagcttcg ggagcacctt gtgcacgtca cccacgatgc cgaggtcggc
    33661 gaccgagaag atcggggcct cgggatcctt gttgatggcg atgatgttct tggaaccctt
    33721 catgccgacc aggtgctggg tggcaccgga gatgcccgca gcgatgtaga ccgtcggctt
    33781 gacgaccttg ccggtctggc cgacctggta gctgtagggc acccaaccgg cgtccacgat
    33841 ggcgcgggag gcgccgggcg cagcgttgag ggccttggcc aggtcttcga tgagctggta
    33901 gttcgccgcc tcaccgaggc cacgaccgcc ggcgaccacc acggcagcag catcgagctg
    33961 cgggccggtg cgctcttcga cgtgacggtt gacgaccttg gcagcaccgg cagcacccgt
    34021 gtcgggaacc ggcagggcct cgaccgaggc cgcaccgccg ccgctctcct ccgccgcgaa
    34081 cgacttcggg cgaacgagga acacgccaac gcccgagccc gtgaacttgg tgtagacgtc
    34141 ctgggagcca ccgaagatcg gctcgacacc ggcgagggcg ccgtcgcgct cgacgaggtc
    34201 ggtcacgttg gtgatgacgg ggacgtcgag ggccaccgag aggcgaccgg cgatgtcacg
    34261 gccgtcctgg gtcgtgccca ggaagatggc gtcgggggcg gcggtgccga cagcggcggc
    34321 gatggccgca gcgactggca caccgggaag cgagtcgccg aggtcgccgg tggcgtgcac
    34381 cgcggtggcg ccatgggcgc cgagcacgcc ggcgatgctc gaggcatcac cgccgaacac
    34441 ggcctcgacg gtggtggcga gctcgcgagc cttggtgagc atctcgagcg tgatgggggc
    34501 gacttcgccc tcgatggcct ctgcgaggac ccagatcttt gaaagcgtca tgatgatctc
    34561 ctcagatgac cttgagattg gacaggaagt cgacgacctt cgtgaaggct tcgccgtcgt
    34621 cctcgatgat ctcgccggcc tcacgctgag cagcggtggc gacctcgatg atctcctgac
    34681 cggcgccggc ccagccggcg ttggaggcct cgatgccgat gtcggcaagg gtcaactgat
    34741 cgacgggctt cgacttggcg gccatgatgc ccttgaagga cgggtagcgg ggctcgacca
    34801 caccggcggt cacggagacc acggcgggga gcggcgacac gacctcgtcg tagccggcct
    34861 cggtctgacg gtcgaccttg acggtgtcgc cgtcgatggt gaccgacttg gcgaacgtca
    34921 ccgacggaag gccgaggacg gcggcgatct gctcgggcac gacgccggtg tagccgtcgg
    34981 acgactcggt cgccgcgagg acgaggtcgt actcgccggc cttgccgatg gcggcggcca
    35041 ggaccttggc ggtcgtgagg gcgtcggagc ccttgagggc gtcgtcgctc accaggacgg
    35101 ccttggcggc acccatggcg agcgccgtgc ggaggccgga gacctcgccg ttcggcgcca
    35161 tggagacgag ggtgacctca ccggaatcgc ccacgagctg cagcgccatc tccacgccgt
    35221 agctgtcgga ttcgtcgagg atgagcttgc catcccgctt gagggtcttg gattcgggat
    35281 ccaacgcgcc cgggtcagcc gggtccggga tctgcttgac acagacgacg atgttcatgc
    35341 gcacagtctg ccaaccagcg gcgggcgctc acaaaacggc acgacgccgt cgaccggacg
    35401 aggttcgccc gagcacggct acggttggca cgtcatgaag gtcctcctca tcgtgaacgc
    35461 ggcggcgtcc tcggtcaccc cccgacgcca ggtggcggtc caccggctgc tctccgccgc
    35521 ccacgacgtg cggatggtcg agacccgcca ccggggtcac gccaccgagt tcgcgaagga
    35581 aggtgtcgac cacggtgtcg acgtcgtcgc cgtgttcggc ggcgacggca ccctcaacga
    35641 ggccgccaac ggcctcgtcg gatccgacgt cgcgctcgct cccctcccgg gcggctccac
    35701 caacgtgttc gcccggacgc tcgggctgag tgaccacccc gtcgacgcgc tgcgcacgac
    35761 gctcgtcgcg ctgagcgaga agtccatcca ccgcatcggc gtgggctcgg tgaacggacg
    35821 gtacttcctc ttccacacgg gcatcggttg ggacgcccgg ctggtccgcc aggtcgagaa
    35881 gcgggcacac ctcaagcgtc gcctcggcca cggcctcttc gtctgggccg gcctcgaaac
    35941 gtggttccac ctctacgacc ggaccgtccc ccatttccgc gtcgtctacg acgacggcac
    36001 gacgaccgag cgcggctgct tctcgatcgt gcagaactcg aacccctaca cctacgtcgg
    36061 caagcgcccc ttcaacctgt cgcccgacgt cacgctcgac gggccgctct cggctatgac
    36121 gctgaccgag ctcaagtcgg ggccgttcat caagctgatg ttcgagacgc tgcggtcccg
    36181 cgacgccatg ctccgcagcg acatcgtcga ctaccacccc gacgtgaact ccctgacggt
    36241 cgagatgctc caccccgttc cgtaccaggt ggacggcgac gatctcggcg acgccattca
    36301 cctcgagttc cggcacgtgc ccgcggcgct cgacctcgtg gtcccggtcg ggttcggcac
    36361 atgatcgccg cgacgacctc ggggctcgtc gccggcggtg tggtgctggt gctggcggtc
    36421 ggtctcgccg ccatccaggc gcggcgccgg accctcagcc gcggccgaga gcggacaacg
    36481 ccaccgcagg acgattcgtg atcacggcgt cggcgcccca ctcggccagc tgacggatcc
    36541 gatcgggatc gtcgacggtc catacgttga cggcgagccc cgcgtcgtgg gcgcgcgcga
    36601 tcgactccga cgagacacac gggtcccacg ggttgaccgc cacacacccc gatgcgaccg
    36661 ccgcggcgac cgcgtcgacc ggttcctcgg cgctcaggac gaggtagccc gtcgcgaggt
    36721 gcggggcgac ggcgcgcacg tggtcgagcg tggcgaggtc gaagcagctc acgaggatct
    36781 cgaggccggc ggcgacctcg gcgatcagct cgaccgtcgc gctggcgagc tcgccggtcg
    36841 ggtcccaccc cggttcggcg ggtgagttct tgatctcgac gttgacgccc atccccgcca
    36901 tcacaccgaa ggccgcctcc agccggggga ccgagatcgg aacctcgtgg tgggccaggt
    36961 cggaaatggg ccgcccgtcg ggcaggacgg cgtcgtggtg gacgacgagg acgccgtcgg
    37021 acgtggcgcg gacgtcgagc tcgacccagt cggcaccctg agcggccgca ccggcgaacg
    37081 cgtcgagcgt gttctcggga aaatcggccg atgcacccct atgcccgatg accaccgtca
    37141 tgtgcaaatc ctctcgaact tttgctttac aggtctgtaa ctgagaagta acttgagctt
    37201 gttccacttt tcacaagagc ccgaccttgc agtaccggcg ggaagacttg ggaagacacc
    37261 ggaagccctc tccgatggtc cggcgctgcg tggaacctag atgaagtcgc aagtttgtcc
    37321 tcacccgcct cgcacgcacc accggaggaa tcgtggcctt gacctcgagc cgctcactca
    37381 ccctcgccaa cgacgattgg cgctcggttg ctgcctgtca ggacaccgat cctgacctgt
    37441 tcttcccggt gggcaccacc ggcccggcga tcgagcagat cgacacggcc aaggccgtgt
    37501 gcgacacctg ttcgtcgaag gccccgtgcc tcgagttcgc catgatcacc aaccaggact
    37561 ccggcgtctg gggtggcacc tccgaggagg agcgtcgcaa gctccgccgt gcctggctcg
    37621 ccagccagcg caagagcgcc tgatcaccga ccttcgccca ggccgctccc tgcggagcgc
    37681 ctgacgcttc ggtacctcgg ctgatccgag gatcccacaa ccctcgccga ccggcggggg
    37741 ttgtgtcgcg tccggggacg gtatctaccg cagcgatgcc cctcatctgt ggcgggggag
    37801 gtcgggccgg ccccctactc ccaacgcttg tgggacggca ccgtcacctc gacgcgggtc
    37861 cccgtcgatg ccccgccgac ccggtactcg atggtgcccc gcagctcgtc gcgcacgagg
    37921 gcggtcacga tcgactgacc gaggctcccc gccggcgccg gtgacacgtc ggggtcgaac
    37981 ccgaccccgt tgtcggccac caccacgccg atgtggcggt cgtcgtggcg cagctcgatg
    38041 ccgacccgcc gttcggtcac ctcgaccgaa tcggggaacg cgtgctccac ggcgttctgc
    38101 agcagctccg tgacgacgag cgacagcgac gtcgccacct ccgacgacac cactcccccg
    38161 tcaccgtcga tgtcgaaccg gatggggcgc tcggccgaca ccagcccctc gttgaccata
    38221 cgaaccagcg gcttgagcac ctcgcggaag tcgacgtcgt cgccgggctc gcgcgacaac
    38281 gtctcgtgga ccagggcgat ggagtggatg cgccgcaccg actcctccac cgcggctcgc
    38341 gccgtcggct cggtcagacg ccgaccctcg aggcgaagca gcgacgagat cgtctgcagg
    38401 ttgttcttga cccgatggtg gatctcgctg atcgtcgcgt ccttcgacag cagcagacgg
    38461 tcacgctgac gcaggtcgga gatgtcgcgc acgagcgcga cggcgccggt gatccgctgg
    38521 ccgtcgggcg ccgactcgag gaacggcagc acccggagcg cgatcgtgac gtcgtcgggc
    38581 cgctcgatgt cctcgacccc gggccggccg gcggcgaggc tcacccgaac ggcggtcgaa
    38641 tcgatgccga cctcggcgag ggtcatgcct cggatcaccg cctcgacacc ggaacgggtc
    38701 aaggtcgacg tggcgttcgg ggatgagtac tcgatggccc cggtcgggcc gacgacgatc
    38761 accccgtcgc ccacacgggg ggtggtcgtc atcccgacgt cctcgggcgg gaacgggaag
    38821 gcgcccgaca cgatcatcga actgaaccgc tcgaacaccc gctcgtaggt ctgctcgagc
    38881 tcgccgggcc ggtgccggaa ggccgagacg aactcgcggg cgagcacggc gatggtgcgg
    38941 ccgccctggc gcaccgggat ggcggccacc cggacccgtt cacccagcca cgccgaatcg
    39001 atgtcgccgc gccggatgac cccggtgcgg aacgcctcgt cgatcagcgg ccgctgctcg
    39061 ggggtacgca cctgcccgac gagttcctcc tggtagatcg tctgggaggt cgccggtcgg
    39121 atgtgcttgg ccatgaggta gcggccgtcg cggtcgagca cgtacagcac cagatcgctg
    39181 aagcagaagt cggcgagcat ggcccactcc cgcatgagcc gacccaggtg gtcgagggcg
    39241 gcgtcgtcca gggtgctgcg cgacgcgagc aggtcggtct gcggaggacc catcagacgt
    39301 ctaacgccac aaccgctcgc ccaacgcgag gaggccggcc aggtcgccga tgtcctcact
    39361 gaagtacggc acggttgcga ccgtgcccgc ccccaccgac aactccgagc gcagctcggc
    39421 ctcacgctgc gccaccaact ggtagttgac gaagctcgtg ccgatctcgt ccaggatgcg
    39481 aggaccgaac ggcagatccc cccacacgtc ggtcaggccg gcggccagct cgtcggcctc
    39541 cgcgaccatc cgtcgggccg ccgtggcggc cccccggtcg agcaggtagt cgggcagcac
    39601 cttgttgagc acgagggcac caacatggag cttgcgttcg ccgagcagat cgaggaagaa
    39661 gcgggcttcg cgcgccggcg ccgcctcgag ggtggacacc accacgaagc tcgtgctcgg
    39721 gctggccaac aggtgctgga ccgcgtcggc ccgctcgacg aacccgtcgt acatcgactg
    39781 gaacaggatg aagaactcgg cgatgtcctg caggaactgc gtgccgagga tgcgatcggc
    39841 caccgaatag aagggcttcg aagcgaagct cacgaccttc gagcggtacg gcgcgatgat
    39901 ccagcgcagg agcttggaac tgaagaagtc ggccatccgt tcgggcgcgt cgaggaagtc
    39961 gaccgcgttc cgcgtcggcg gggtgtcgac cacgatcagg tcgtagcgac ccgacgtgtg
    40021 gatctcgtac aaccgctcca tggcgacgta gtcgtggctc tggacgaact tgcccgtgat
    40081 gttctggtag agcgggttcg cgaggatctc gtcacgggtc cgggtgtcgg gcgcgtgccg
    40141 ggcgatcagg tcgtcccagc tctgcttcgt gtcgagcatc gccgcccaca gctcaccctc
    40201 ggggtgcacc ccgatctcgg cgaacgcctc ggccggcacc ctcgtctcga cgttgccgaa
    40261 gctctgcagc cccatggcgt tggcaaggcg cttggcggga tcgacggtga gcaccagcac
    40321 cttcgcgccc atggtcacgg ccgcctgggc accgagggcg gcggccgtcg tcgtcttgcc
    40381 gacacccccc gagcccgtgg acaccaggat ctccttggcc gcgcacagct gctcgatcgt
    40441 gaccggcgcc ggagcgacgc gcttggcggc cactagagca gctcctcggc gaggcggtcg
    40501 gcgacctggc gagtggtccg gatgccgtgg gagcgggtga acaggtaggg cacgttcacg
    40561 aggggcaccg tgtcgggcag gcgactcgcc aggttcgcga ggtgcaccgc gcggctccga
    40621 cgcagatcgg cggccagccg ggccgcgtcg accacgaggt cggatcctgg cccgaggagg
    40681 gcggcgaggg cggcgcgtcc gggctcggcg tcgagctggt cgaacgccgc ttcctcgtca
    40741 cggctgaaga gctccgggag cacgcggttc gcgatcaccg ccgccacgtt gatgtcggtc
    40801 tccgaatcga ggcggtcgat cagctcgatg gtctcgttga cgggcatctc ctcgggggtc
    40861 gccacgacca cgacaccggt ctgcgccggg tcggtgagga tgttgagcat ccactcggtc
    40921 tgttggcgca cgatgccgac gctgacgagt tccttcatcg cgaccggtga cgccaactgg
    40981 ccgacgacat ggccggtggc cgacgcgtcg accacgacca tgtcgtagtt gcgttcgcgc
    41041 acctcgtagg ccaacttgcc gacggtcagg atctccttga cgcccggcgc ggccgtggcg
    41101 acgaaatcga aggtcttcgc cagcggcccg agccggctga tcagggggag gtggagttgc
    41161 agccgcaggt actcccgcag tgactcctcg gtgtccatcg tcatggcgaa caggccggac
    41221 tggacctccg tgggagcgaa gcggagcggc ttggtgccgt agaactcggc caggttgccc
    41281 ttggcgtcca cctcgcagac caacgtccgc ttgccctcgt tcgccgcgag gagggccagc
    41341 gacgacgcga tcgtcgactt gccgacgcca cccttgccgg tgacgaacag cagcttgcga
    41401 tcgaggaggt gtggtctcgt cgtcatgaac cagccgaggt accggagcga tagtagggag
    41461 cg
//
LOCUS       DAOSOA010000004        37741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_1129_length_37741_cov_7.871650, whole genome shotgun sequence.
ACCESSION   DAOSOA010000004 DAOSOA010000000
VERSION     DAOSOA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 37741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37741
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_37741_cov_7.871650"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKY13_00725"
     CDS             complement(<1..354)
                     /locus_tag="PKY13_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78472.1"
                     /translation="MRASTATRGVIMLLQGLLSFRVVMALATSASVTAAVAGPMAYQA
                     TKARDEVVAAAPTTTTTTATTVPTLDPPPRSVPNAVTSTTSTTTTTTTTTTTTIAGQP
                     APTTTTTIAGQPAPTT"
     gene            complement(392..1027)
                     /locus_tag="PKY13_00730"
     CDS             complement(392..1027)
                     /locus_tag="PKY13_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT78473.1"
                     /translation="MLKILLADDHQILREGIRRGLEGAGESVVGEASDGEEAIELARS
                     TNPDIVLMDLSMPVLDGVEATRRIATELPDVRVLVLTMHDDPGRTRAAIAAGASGYLT
                     KGTSFAEVLQTIRLVASGETFLSPALAVSMLRAAEDAAATEDLLSERQVEILQKVANG
                     YGTKQVARDLGITQKTVHNHLNAIYRKLDTQSLTHAVLSAVRLGIIHLDQN"
     gene            complement(1029..2852)
                     /locus_tag="PKY13_00735"
     CDS             complement(1029..2852)
                     /locus_tag="PKY13_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOT78474.1"
                     /translation="MPEHGKSILNPSATSPSSPNEFGARSRATAPVPSSVDMVAALRA
                     LPFGGVDETARNLDVSPQIQATISPVINAFRWAAVMYGMIFGAAAAFKGSYSVVATLS
                     VCLFLTCWRTMIPIRLGSRVRLDRVVALTDVLILGLAVGASGGPVSPFVFCVMAGIAV
                     AAFGWGYLDGALAFVIGWAAMIVGHAIHPAGETVTLDRAVGLLLGSMVLVALGSAFLR
                     TRLIEAEERRRRLAGQVDTLAETNDLLTMLNAVARTLPTSLNQREAIDAARQQVIDTF
                     RSNVICLLEYDEAHDEWIPKLTEGCAVKPTSSVDELPVHLRQAMDSREPWLVPDLTAG
                     SLPGISPTSGSGLYTKLESRGRVVGVLGIEHATPGQYRDRDRRLLLGMADVVALTLDN
                     ARWFGRLRSLGAEGERARIARDLHDRLGQWLTYISFELERFISTDGSGFVQLTQLYAD
                     VQTALGELRETLRQLRSGITEDRPLSMVGKELVDRYAGRTGLEVTFRVTNPTEKMPVP
                     VENELLRILQEALNNVDKHAKASRVDVTWAVDAGTASLVIADNGRGFDAAKGVRDSAY
                     GLVGMRERVDVIGGRIAIESAVQQGTTVSVSVGAIPVAKEG"
     gene            3110..4033
                     /locus_tag="PKY13_00740"
     CDS             3110..4033
                     /locus_tag="PKY13_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT78475.1"
                     /translation="MTDDIEVVHYPGAKRPDRRETLDAAGVAISKVEWGDPSAPPLLF
                     AHGGFDFAETLNVFAPLLVAQGWRVVSWDHRGHGHSAWADLYSWEADLRDAATVLESI
                     GPAPLPMLGHSKGGALTLQLAEAFPHRFTHLVNLDGLPSRRSMPDIAEHERTRMLAQE
                     LGAWLDHRRRVSDKQRRPGTIDELATRRGRMNTRLDHDWLRYLVTVGGRHDGDGWRWR
                     IDPTLRMGGFGPWRPEWSMLRLPGLSMPFLAVLGLELEVMGWGTRPNDVLPYLPPGAH
                     FEQLEGIGHFMHIEQPQLIADMVIDFVGAPE"
     gene            4030..4695
                     /locus_tag="PKY13_00745"
     CDS             4030..4695
                     /locus_tag="PKY13_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT78476.1"
                     /translation="MSGTDSEPTAWVRHGRSDLALQCLREGEGRALLLLHGLGERAPT
                     VAPDWVAPWPGPVWGLDFTGHGRSTLPRGGGYTAEMLLGDADAALEHLGEATVVGRGL
                     GGYVGLLLAGARAARVHGVIICDGPGIAGGGIQPGSPFVVFPRFGHGETPDPFAMVEL
                     ARDVRPPDYALNYVRFAFESAPVPDPVVVAAVVRPEWLSAVAVEPGVIECERSEALAS
                     FID"
     gene            4770..5984
                     /locus_tag="PKY13_00750"
     CDS             4770..5984
                     /locus_tag="PKY13_00750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT78477.1"
                     /translation="MSEGLAGGDSVLEPDRDDAIGVMSAEFVDPALEREFQLHHLDST
                     ARRAISTSVVGASSCFVFTVSDFTRLGFGSHFVLLLALRSLVLLASVLFAMAVRRNPV
                     RSLSWKFVSTLESAVLAVLFVVMAFRPEETEVNNLGISVTIVAMFLLVPNRFPAAFAI
                     SVATVVIDLGLAVTLVDSPLSRQIPSALTLVSFLVIGAMSANQLQRARREEFLSLRNA
                     RTSNDLLVEEIGRRRVLEDELTWMATHDALTDLLNRRAFYELAEREAVRARRTNLPVS
                     ILVIDADEFKAVNDRFGHHAGDEALRRIAATCRQHLRADDVVGRVGGEEFAVVMPGAN
                     SALAHHVAERLRVAVTEAPIDHPDGPINLSVSIGVTEYRVWHEAMLDAIQRADEAMYL
                     AKTTGRNCVVPV"
     gene            complement(5989..6609)
                     /locus_tag="PKY13_00755"
     CDS             complement(5989..6609)
                     /locus_tag="PKY13_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT78478.1"
                     /translation="MSTGPEQPPAARTALSRPAIVAAALQLVSEHGLDALSLRRLASD
                     LGVTAPALYAHVRDKSDLLATLAETGFRELLDRYAAIATDDPIDRIRQQCLIYVDMAL
                     ANPGLFQAMFMFPPQALELPNPRTELAAATEAFEQPTKAIADAIATGAIHPDHDPFVT
                     ALTLWTVTHGLAQVLLLGVSADADARRDLQEAVLGATFRGLALPPA"
     gene            complement(6606..9071)
                     /locus_tag="PKY13_00760"
     CDS             complement(6606..9071)
                     /locus_tag="PKY13_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT78479.1"
                     /translation="MSQAVLDERPTELAAAITDSQLLWGYLTNAALATLLCTPDGAIQ
                     WASPSVYSILGHQPRDLTGVDIGNLIDDTNRPAIRARVQGAARVDDSEPVCCQVFGGD
                     SASVWCDVDVLAIDQSGEPQVLVTIRDASRRVAGTRALRTSEERFRTIVNEAPFGVGL
                     QDRDGNFVFLNARLKQIMGTSANYLLRDELGRHIHEGDIDNALAAWRTAGENDSATTQ
                     MRFIRPDGELRWGSVRILPQLDSAGEITNYLWAVEDITARVRAEEDQARLYTLMEASS
                     DLVIVMDPELMPLYVNPAGRRFLGIEQEALLATVTTPLDFQTRPADALETIAQHMASR
                     EIWHGEVTLRSAAGTWEPFSAQVIPDHQKSGQLNRISAVLRDINQRKQFEERLEWEAT
                     HDPLTDLPNRALLTQRLAEAMDRGAQDGTWVAVVFLDLDNFRIINESLGHRVGDNLLK
                     QTAQKLTGLVWSRDMVARFGADEFAIVMEGLETAEGAEALAERIRKAVSGRVGMAEVE
                     LLMTFTAGVAVTDGTRGDPASVIRDANAALHEAKLRGRDRTEVFNGNLRARAVDRLTV
                     ETGLRRALRRNELRLFYQPQISLDTGRINGAEALIRWQHPEHGLLSPSDFVPIAEQSG
                     LIIPIGSWVIEEALRTLAKWSTELPGGENFTMGINLSGKQLVKPSLIADIEAILEETG
                     VNPNNVEMEITESILLDDVDRSIMVLEKLKALGLKLSLDDFGTGYSSLTYLRSFPIDV
                     VKIDRSFVDGIGNDRDNAAIVRSVIDLASTLGLQCIAEGVETESDLAELRSLGCDVAQ
                     GFLISKPLSEADAARLISYDPTW"
     gene            complement(9178..9390)
                     /gene="xseB"
                     /locus_tag="PKY13_00765"
     CDS             complement(9178..9390)
                     /gene="xseB"
                     /locus_tag="PKY13_00765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT78480.1"
                     /translation="MTDETPGYAAAMAELESILTQLEDDRLDIDHLADHVARAAFLVG
                     LCRERLDTAKLRVTEIVADLDETTGS"
     gene            complement(9387..10736)
                     /gene="xseA"
                     /locus_tag="PKY13_00770"
     CDS             complement(9387..10736)
                     /gene="xseA"
                     /locus_tag="PKY13_00770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT78481.1"
                     /translation="MPDVVTWTIAQLTDWVNAVIGETLGGEIWIEGEISNLQRSNAGH
                     VYFTLTEPADEAGGRAPNTLAVTLFEWHRQNVNRHLRRSGGAVRISDGVRVRIRGSVE
                     VYGARSQLQLKMSGIDPVFTLGNMAAERALLLARLDGEGLLRANAALPLEPVPLRIAV
                     VTSIGSAAHADAMHELGRGGIGFDLVVVDTRVQGADAPASIVAALGRAAAAGVDLIAL
                     VRGGGARTDLAAFDEEIVARAVASSPVPVWTGLGHEIDRTVADEVAHRSWKTPTACAA
                     AVVELVVAGAQQLDDLWSGIEDATARRLERSAIRLDRLASRTARTALADLDRSSSHLA
                     GAAHRVRRLADAHVIRGSARTDDARRRLVAAAPRAIGDQGRRLDAIAATVRAYDPAVS
                     LARGWSITRGADGRVVRAADLDEGDVLVTQVADGRITSVVTASSSGIASDGATVPGT"
     gene            10798..12348
                     /locus_tag="PKY13_00775"
     CDS             10798..12348
                     /locus_tag="PKY13_00775"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT78482.1"
                     /translation="MAEANEGNETSGARRWSRQSSGTARARSTVRGVLGFQLLANGLG
                     LSVILLYVTLLFPPGNDEQESALNLKVFGAYLAVNLLVGLPVNQLLLRRAVVWVREGT
                     EATARQRWLVLRLPLLETGTALVSWFGGAILFGIINESARRVSVGIALAGVVTCTLLY
                     LLLEGHFRPVFALALRKADLPEGRRDVWPRLMLAWLLGSGMPIALLGAAPLLDERFDS
                     SRLPWIAAFGLLGGGAVMTTAAISVSRPLNRLRGVLRDVEQGELDIDVPVDDLGELGR
                     LAEGINDLVAGLREREQLRDVFGRQVGQPALAHLPGDTESFDAAGEQRDVTVLFVDLQ
                     GYTRFSESASPEAVVAMLNRFFRVVVAAVNREGGWVNKFEGDAALCVFGAPQPDESHA
                     ANALRAAEALRRDLARGEHELLAGIGVATGTAVAGFIGTPERYEYTVIGNCVNLASRL
                     CDLAKEERSGVLASLDTWVAAGRPEGWLDVGRVRVRGRSQKLTAVTLASGRRSRRETS
                     GRFRPSDR"
     gene            complement(12329..14161)
                     /locus_tag="PKY13_00780"
     CDS             complement(12329..14161)
                     /locus_tag="PKY13_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78483.1"
                     /translation="MTYSDQEAAKRKLDRGLVRRVGHYAKPYQGMLIGFLVTIVLEAL
                     LSLAPPLLFKAIVDNAIADKDTGYLTQLAALVVAAAVGVSLLGLAERYWSARIGEGLI
                     YDLRSELFDHVQRLPMAFFTRTQTGSLVSRLNNDVIGAQRAFTGTLGTVVSNVITVVV
                     TLAAMARLEWRLTIASVLLLPLFIWPAKRVGKRLATITRDGMQENAEMNNIMTERFGV
                     SGAMLVKTYGNEQRELTEFSGRAAKVRDIGVKSAMLTRIFLSVMGLVGAMGTAAIYWW
                     GGRQVINGVITLGTLTALAALVVRIYEPLTSLTNARIDVMSAFVSFERVFEVLDTPTP
                     IQDAAQPVDLGRAKGRIEFDDVTFHYPAASDTSVASLEGNFAPQTDELATVLHGVTAT
                     IEPGQMVALVGPSGAGKSTMSSLVTRLYDVSSGAIRVDGTDVRDISLASLRSSIGIVA
                     QDPHMFHTTVGENLRYARPDATDSELRDACKAAQVLDVIERLPDGFDTVVGERGYRLS
                     GGEKQRLAIARMLVKDPAIVILDEATSHLDSENEHLVQMALATALADRTSIVIAHRLS
                     TITAADQILVMDSGRIVQRGTHATLVTAGGLYTDLYRTLTGPTA"
     gene            complement(14293..14377)
                     /locus_tag="PKY13_00785"
     tRNA            complement(14293..14377)
                     /locus_tag="PKY13_00785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14341..14343),aa:Ser,seq:cga)
     gene            complement(14420..14902)
                     /gene="tadA"
                     /locus_tag="PKY13_00790"
     CDS             complement(14420..14902)
                     /gene="tadA"
                     /locus_tag="PKY13_00790"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOT78484.1"
                     /translation="MPIDAAVAADPERFMRLAIDAARAAEAAGDLPIGAVAVRDGDVI
                     ATAGNRRAIDQDPTAHAELLVLREAAAVVGEWRLGEVTVVITLEPCPMCAGAMWASRI
                     GGVVFGAVDMKAGATGSLYHLGSDPRLNHEFPTRAGVLGEECASLLTTFFASRRTTEI
                     "
     gene            complement(14986..22800)
                     /locus_tag="PKY13_00795"
     CDS             complement(14986..22800)
                     /locus_tag="PKY13_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5979 domain-containing protein"
                     /protein_id="HOT78485.1"
                     /translation="MNRSITTASRRLGVIALCAFVGLGGLPMVPTVGEAAADFGAGQS
                     ARYNKNVNGDFLMVGNTVLNCSGSSCTTNSTTNDDLNMANNDPDGNGALFNGSSATFT
                     IPSGAVVDAAYLYWGGNLGTTRSNGTEYYCSDNHAEVSNATANKSSANTVRMKVGAGA
                     YTTVTANTVYTVPSGGLAQALPSGSSDDGLVYEGVTDVTAAFSTVAAATATTVSVANV
                     QTMQGENCHAGWSLTVVYRFPTLNCLTGANDDAGAGANHRNDYRNVAIYDGLIQQQNG
                     AADTTTTLSGFLTASPGPVALRLGVLAWEGDQFLDEDQMKVKSNLSGSSTTVNPAGPS
                     GTDNFFDSGKQVSADHNADLDADPDSGASIAQGYSGGRGDGHGIDAKTQVVQVPGGTS
                     SIDVTFTTDRDNYYAGQFALSSPLKCLLIVEKDQAVNGTAVGRDNSTSPSPYVKSGDL
                     LTYTMPVRVAGDVDLNNVTISDSIPAGTTFVPGSLSVGKGQTSAAALAALASGGSFGS
                     GTVTANLGTLNNLTGASCAGGELCFGVVRFDVTVNAGVAPGTVITNVANATFTASGIA
                     INEISNPVTDRVGALLTISKTIAGAIAGDPTTFTFTVACGGNQIAGSPVSLANGGSVT
                     LGVTPGASCTVTEATNANFAVTVTGAIVTNGGSTTMNQDRNVAFTNTRRFAKVVVNKT
                     TNAVAGDPSGSPTFEFTVACPGVARYPKTLNISGNGTAETPSDIPFGASCTVTEATTP
                     GWLQSGSQSVQPVDQAAESVSFTNTRQTGSLIVNKSTVGGDGTFDFTVACDGTAWDVT
                     RSVTTSGGTGTSTAVTGIPSGLNCTVTETVPAGWTQTAAPAGPITIVAGQQATASFTN
                     TRQTADLVITKAVTGTSVLPVSGSFDFVADCGAAGTFSRTITASNSANGTATIAGVPV
                     GTSCTITEAVPAGWTLDGSVAGNVTPRQITVASQGNNVTFSNRRDVGTITISKTIDQG
                     AGTFRFDLTCGGAPVPGSPFDITINAPSTAGSVTVSNVPTGSNCVVDENPNGSVSGDF
                     FQVTPAANGTVGFPSTGAAQTASYVDHRRVGDLLIGKVFPPNSLGDPDQVFTFQWDCG
                     PQPRTIGLKAGQQHTVSGIPTGTACTVSETANAGYSSAVAPAGGAVTIADGTNTVTFT
                     NTRKTGTLELRKELVPSTDPGRFDLDIDGGPHEVAGVGDDGTTGERTVPVGSHSIAET
                     VAAGNAANLSDYTTTVACVDTAGTTPVLVTKDDNVVVDEGAKVVCTFTNTRKTGNLTL
                     TKTVVDPLNSGHSFDLRAGGTTVVAGATNGSTGSTTVNTGPVTVSEAGGDLGLYSSAI
                     VCNSAGQPIAAGTGTSLTVNVTAGADIVCTFTNTRSTGTLEVRKQLSPPNDPGTFDLS
                     IDGTVRTAGAGHNGTTGPVVVAAGTHSVAEAGAGASGTSLADYAAALSCIDTAHGSTA
                     VAVSDGTVQVDPGDAVVCTFTNTRSTGGLTLRKVVVDPVGSNHTFDLLAGADVVVDEA
                     VDGSTGTTTVNSGPVTVSETSGALGRYTTAIACTSGGQPLASGGGTSLTVEVSAGSNV
                     VCTFTNTRRTGTLEVRKQLVPTNDPGTFDLSIDGTVRTAGAGHNGTTGAVTVAQGPHS
                     VAEAGAADTKLGDYAASLSCVDSANGNAPVIPTAGSVQVDAGDAVVCTFTNTRTTGNL
                     TLTKVVVDPLASSHTFDLLAGAKVVVDEAVNGSTGSTTVNTGPVTVSESGANLGQYAT
                     SIVCTEDGEPVARANDASSLQIDVEVGANVVCTFTNTRRTGTIEVRKQLVPANDPATF
                     DLSIDGTVRTVGAGHNGTTGAIVVIPGVHAVAEKGSPTNASLDYLPTLACRETVTNAP
                     LVVTNGAVAVASGDVVVCTFTNTRATVGITVEKVVVDPLESGHTFDLLVDDTVIVDDA
                     VDGSSGTTTVDTGSVTVSETGADLDGYDSAIACTAGGTPIAYGFGASLDIMADGSGAV
                     VCTFTNTRRTGTLEVRKQLSPTDDPGTFDLSVDGTVRTAAASHNGTTGAVTVAPGIHA
                     IAEAAAGSTPGGEYSATVACIDTADEGSTVDVEAGNVEVSADAAVVCTFTNTRKAADL
                     TITKSPGSSTVLPGGVATFSITVTNAAGGGTAHDAIVNDPLPAGVDYLASQGGVCGET
                     GTSCSLGDIAPGTSVTFTISYAVGTSPSLMVVHNEATVTSPDDPTSPGDDADIPVARL
                     RIEKSGDPGSYASAGDVLTYTYVVTNVGGAALTGVTVADDRIPLASIDCNGDGPGNGQ
                     PFAMAAGVSVTCTAADAVSAADLTEDSVDNVATADSDQTPPVIDTWSIPRAALSIDKV
                     ATSTGPYALGDTITYAITVTNTGVATLNGVRIEEAGVGAVLSSGCAAAPSGPGVTLAS
                     GEKLTCAATHVVTQADFNAGSYVNTAVASSDETPDVSDAATVPTPDPAVDLAIVKALT
                     SSSLVAGQEATYSLTVRNKGPATASSIKVTDTLPAGLQPLTATGSGWTCALDGTTVTC
                     STAATLASGTTLPAIALTVAVAAGARGTITNTGTVGSTQVDRDATNNTSSVTNPVTVV
                     ESEVQVPTTTTTTTSPPAVRRAVETRPSTGSPLATTGTTVSVLLLAAGLAMVAGALVL
                     AVRRRRSR"
     gene            complement(22963..23625)
                     /locus_tag="PKY13_00800"
     CDS             complement(22963..23625)
                     /locus_tag="PKY13_00800"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HOT78486.1"
                     /translation="MTHAGVTPAQYLDPDIASGLPWRDLPVVDGRDPSLAELLAIAEG
                     DPAEFEVPIVPWPLSGPRPLDPGTGIEGGTTEGTFLTVWDGQRLRARNAAVGGDYVIG
                     LGCAPESLDAPSSGPREQILLWHLNHHPDGGQLFWSVDGRPFLVPVVPVGDDPDLERA
                     VVARSDGSFGICVLPGVWHDGVYPESGDGDFRTRQGRVHARVSADVAGEFGCLLRIPL
                     SH"
     gene            complement(23703..25553)
                     /locus_tag="PKY13_00805"
     CDS             complement(23703..25553)
                     /locus_tag="PKY13_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOT78487.1"
                     /translation="MTDAPASTRPTTADARLSSLFPDPDGVVPFWRTRRARWGLVGLT
                     TVALVASGFVAVNASGSEALNYRTAVAGTHDVQAVLNGVATVEAVNQAAVAFPISGTV
                     ASVAVAVGDEVVTGQTLATVDTASLERTLHTRQAALAQAELTLSKALDGEPTSTGGAG
                     AAPTASSVTGARTATTTVTTAAAATATPSPASTTTSGSSASGTAATDAAALRYAQQAV
                     LDAQRTVDAALTDSEATYQSAAAVCDSTETTTTTTTTSPSTTTVPPTVPSTDVLAACR
                     SALSTVRSAQAATAAAQTTLSSATQELDRLLSASTAAVTAGGGSTAGASGAPVTTTTA
                     APASSPLPAAAATSSGTSSTPSSEQLIALQKAVDAAALDVIVATHNVAQTELTAPISG
                     TVVAVGFDTGESVTAASTTQQIIVVGDGGFEVTTTIPVAKIPTVEVGQQATVTLDSSD
                     EPLSSEVVSISVTPDSASTSTAYRVVLALEEPPTDLHSGATGSVSIVTGATREALAVP
                     TSAVTTNGTEHTVLVRTATGTKSTRVEVGVVGTTWAEITSGLTSGQRVVLADLDAALP
                     GSATAESAGASNTSGSTPTGFPGGGIPDGGFPGGAGFTGGTPPAGVRPGG"
     gene            complement(25557..26792)
                     /locus_tag="PKY13_00810"
     CDS             complement(25557..26792)
                     /locus_tag="PKY13_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT78488.1"
                     /translation="MSWRDTLRSAFEAVRTHRLRSALTMLGILIGISAVVLTVGLGQG
                     AQAQINDQINELGTNLLVVSPGSTTDASTGTRGGFGTASTLTTTDVAALESNNVAPDV
                     QAVAGVSTTSVSLVQGTTNWTTTLTGTTESWSEVRSRDVSTGRFLADTDEADAAAVVV
                     LGPDTATELFPTGNAVGQTVSYNGTKLTVIGILDELSSSESTSNNDLAIVPLSTYTQR
                     LVGGTNRNSVSSIYVKATSADTLSAAYQETDRLLLNTHGISDSSDADFSIATQESILT
                     AASTVDDTMTVMLAGIAIISLLVGGIGVMNIMLVSVTERTREIGLRKALGGRPQLIRR
                     QFLVEALILGLAGGILGVALGIIGAEVLPHFTDSQVLVSIPACLAAIAMAMAIGVGFG
                     VFPATRAAKLTPIDALRNE"
     gene            complement(26789..27538)
                     /locus_tag="PKY13_00815"
     CDS             complement(26789..27538)
                     /locus_tag="PKY13_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78489.1"
                     /translation="MTATTVRPTRPVTPPGTVPVIELTDVTKLYRTGDVEVAALAGVT
                     LRIEQNEFVAITGPSGSGKSTLMHILGCLDVPTGGQFRLAGHDVGTLDENHLADVRNL
                     LIGFVFQQFNLLAYLPAWRNVELPLIYAGTPPAERRQRAMAALDKVSLANRANHRPGE
                     LSGGQQQRVAVARALVTEPALILADEPTGNLDSTSTDDVLGLLEELHTDGRTIVLITH
                     EPDVAERAERVIQVRDGRIWAPGDSMPAEPT"
     gene            complement(27535..28854)
                     /locus_tag="PKY13_00820"
     CDS             complement(27535..28854)
                     /locus_tag="PKY13_00820"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOT78490.1"
                     /translation="MPAIALVAVAVWWFGIREDTYTTTTATAVAPTNQLVEVTSGSMN
                     ETVSAEGTVAAAQTDDLSFSSSGTVTAVNVAAGDTVTAGQVLATIDSSELAAAVTSAE
                     SAVADAEAKLADDQDADASDEQIAADIAQIATANDTLATATEALAGASLVATFDGTVA
                     SVDLTVGEELSSGGQGGTTLTGSGTGSGRSSATGGGSTAALPSVNQSTGSSSSTAQIQ
                     VVSSGRFEVEVSVDSTDIDSIEVGQTVDVAVTTSSSNRRGGFGGFPSGGLPSGGFPTG
                     GFPTELGGGGSGTGTTQSGASGATATGSVTEVSRIADASSGVAAYQVVVSFTGATDQF
                     YVGSSVTADIVTAERTDVLQVPTRAVSTDDTGSSVTVSTDGSADGATETRSVTIGLTA
                     SGMTEITSGLKAGEQVVISFPGRGVGNAAAGAPTGAPTGAGATGASR"
     gene            29103..30119
                     /locus_tag="PKY13_00825"
     CDS             29103..30119
                     /locus_tag="PKY13_00825"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HOT78491.1"
                     /translation="MSYESMPTQPPTDPPQPPSATPVQPAADAKPSGPTAKTWIIGGV
                     GAVVIAAAAIVGINAASSDTTTVDTAAATNGTAANGSNGAAADGQGGFAGRGGGTFGT
                     IESIDGATITVASQDGTSTKVVTTDATTVTSTGEGTAADLKVGDNVQVTGSGTSSDLA
                     AERVVDSGDVAATGGFGGRGQGQDGQNGQGFPGGTPPEGMSIPEGMTPPEGMTPPADG
                     QMPTDGQMPTGADGQQLVFASGTIESIDGSTMVVKASDGTTSTVTLSDTTTISVVTTI
                     TVSDLAEGDTIMVRGETADGTVTATSITEGATGFGGGGFPGGGAPGRPDQSGGATGSG
                     SASN"
     gene            complement(30271..30468)
                     /locus_tag="PKY13_00830"
     CDS             complement(30271..30468)
                     /locus_tag="PKY13_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOT78492.1"
                     /translation="MARPREFDDRVTKAVRLSPQLDERLKALARERQVSVNLIMNWAL
                     EEYVEQAPRVEDLRVRSSRAS"
     gene            30670..30948
                     /locus_tag="PKY13_00835"
     CDS             30670..30948
                     /locus_tag="PKY13_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="HOT78493.1"
                     /translation="MADRQVTHAHRDDQGIILAVCWKGGTSLMYTARQQVVDDISSGA
                     HRYYVHEQAPAVWVEVITVNGVQYIKTEADSTSQNNLDNLPTCTQKAG"
     gene            complement(31483..31725)
                     /locus_tag="PKY13_00840"
     CDS             complement(31483..31725)
                     /locus_tag="PKY13_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78494.1"
                     /translation="MAEEFAGGGVDDAYIEVCDEDDDVGSGVFSSDADVMQPAVVAQR
                     DLAGGVDAVVAGPVVRRRLCRDSGRRAWDTHPFDTP"
     gene            complement(31912..33072)
                     /locus_tag="PKY13_00845"
     CDS             complement(31912..33072)
                     /locus_tag="PKY13_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT78495.1"
                     /translation="MTGTIRSRLRIRAHLDDVGSTVLSRPGRIATVAALTAVGIGSAV
                     AVLGLAEATRVSVSDRFAAQRSTFVTAQLVDTSVELPDDAAQRVEDINGATAAVVLTE
                     LGQRDVSSGGFTSQAPVVRFAGSLEAALDLNVPGQPADADRSIVSGGGAFVGSGVARD
                     LSLGPVGLGPTVSVDGIPCPVLGVITSSPEVPALLTSVLVTRSAEVALGGGSTTIAVQ
                     VDAGYANSVARSVGDALVPNRPEAIEGSAVFDTSSLGGQVDSDVRDLVVVVAAVVLAG
                     AVLAGGLSAYSSVTSRRPEIGLRRVMGARPIHIFVLVLSETCLAGLLGGFTGGWAGAA
                     LVLSIRPAGEPILDPRYLVAAPVLGLTIGLVSGFLPAIGATRISPSAAFRYV"
     gene            complement(33072..33755)
                     /locus_tag="PKY13_00850"
     CDS             complement(33072..33755)
                     /locus_tag="PKY13_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78496.1"
                     /translation="MTLIVDAHDLALTYPGPPIVNAVVNAHVTVANGEYVAVTGRSGA
                     GKSSLLSVLGGLERPTGGKYLLQGIDMIACSEGDRASVRGELIGFVFQSFLLDDRLSA
                     TQNVARGLLYRGGRGDPTERSRAALDRVGLLHRSEADAGELSGGERQRVAIARALVHQ
                     PKLLLADEPTGSLDSATSALVLDILDDLHAAGLTIVCVTHSDEVARRAQRRLVVSDGR
                     VEPSPSHLT"
     gene            complement(33752..35002)
                     /locus_tag="PKY13_00855"
     CDS             complement(33752..35002)
                     /locus_tag="PKY13_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78497.1"
                     /translation="MARWGLVILPLAGFAALVVVALTSSSPSEITERSRVAAKPVTYV
                     VGPRVLQSDLVLRGSKHFRDRADVLAPPTAEGNRPVLTSLDVAVDATVRPCGLLGSVA
                     DRPIIALPGAIQPYRDLRPGDIGTDVSQLQTALAACGHPSDDPPGVFGSSTAQAVLDT
                     YGSIGCTPLTTQGTLGQLLAKRVELDRAESNAESLLRATTGDTAAAKSALDAARAATT
                     EFSRIEGVVVPARELAVVGALPGRVVSIDAALGQSLTDGQTILTIESGPEVVRADITQ
                     SQKALLTAGLDAVTDGQPPIPLKVTRIVSTEATALPEGTAPSTLNVELEPLAPDTLDI
                     DGSEVTLRVALVPPRSADLVVPSSAIVEDEQSDSARVLVLDGKRQRRVAVSVGETVDG
                     WVEIIDTGKLRRGDKVVLGSRGTR"
     gene            complement(35042..35893)
                     /locus_tag="PKY13_00860"
     CDS             complement(35042..35893)
                     /locus_tag="PKY13_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78498.1"
                     /translation="MVSATAVLSAGCGSDDLGSVEKELGSHRDLATYALPLDEVSVSS
                     STWPNYVENVLVKNCMDSKGVEWTIPAAPDVIGAAPKSTNRFGRRIFDLKVASELGYR
                     QDGGATKEQLELNSRKLTDQQQAAFNECVAKMRKEVPAPPQSLVFDYASAAYSSTMAD
                     PEVLDATEKWRRCLSEQGYGQYVEGADNYGDVAVKVLEELYGTDSPTSDEPVAAKEIE
                     VATADAKCQESTGYLKLVYSTEYDEQLRQAVGHESQLRAQREATTHYTKKIDQIAASL
                     GLASGDR"
     gene            complement(35956..36378)
                     /locus_tag="PKY13_00865"
     CDS             complement(35956..36378)
                     /locus_tag="PKY13_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase inhibitor family I36 protein"
                     /protein_id="HOT78499.1"
                     /translation="MAIGRRLGRLAAAAAVAMLGVGAVSLDAGATVSCPGGGYVCVWK
                     DASYQSFGDGAKSGAWSGDDNAYGNDYYYNTTTSPHDSVSSVVNFGNSCNVRMYKDVS
                     GGGASFLQVLGSGDSDLGNASGPVGFNDSLDSHRWCVA"
     gene            complement(36584..37528)
                     /locus_tag="PKY13_00870"
                     /pseudo
     CDS             complement(36584..37528)
                     /locus_tag="PKY13_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611781.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5776 a        12214 c        13409 g         6342 t
ORIGIN      
        1 ggtggtcgga gcgggctggc cggcgatcgt ggtcgtggtg gtcggagcgg gctggccggc
       61 gatcgtggtc gtggtcgtgg tcgtcgtcgt ggttgttgtg ctcgtcgtgc tggtgaccgc
      121 gttcggcacc gaccgcggcg gcgggtccag cgtcggcacc gtggtggctg tggtcgtcgt
      181 cgtcgtcggc gccgctgcga cgacctcgtc acgcgccttc gtggcctggt aggccatcgg
      241 gcccgcgacc gcagctgtca ccgacgcgga ggtggcgagg gccatgacga cgcggaagga
      301 caagaggccc tgcaacagca tgatcacgcc acgagtggcg gtggaagccc tcatccttgt
      361 ggtccttcac ccggcctgga gcgggaagtc gtcagttctg gtcgagatga atgatgccaa
      421 gccggaccgc gctgagcacg gcgtgggtca ggctctgcgt gtcgagcttg cggtagatgg
      481 cattgaggtg gttgtgcacc gtcttctggg tgatgcccag atcgcgggcg acctgcttgg
      541 ttccgtagcc gttggcgacc ttctgcagga tctccacctg gcgttcgctc aggaggtcct
      601 cggtcgccgc ggcgtcctcg gcagcgcgca gcatcgaaac ggccagcgcc gggctgagga
      661 acgtctcgcc cgatgccacg agtcgaatcg tctgcagcac ctcggcgaat gacgtgcctt
      721 tcgtgaggta gcccgatgcg cccgcagcga tggccgctcg ggtgcggccc ggatcgtcgt
      781 gcatcgtgag gacgagcacc cggacgtcgg ggagctcggt ggcaatgcgc cgtgtcgcct
      841 cgaccccgtc gaggacaggc atcgagaggt ccatcagcac gatgtcgggg ttggtggacc
      901 gggcgagctc gatggcctcc tcgccgtctg acgcctcccc gacgaccgac tcgccggccc
      961 cctccagacc acgccggatg ccttcgcgca ggatctgatg atcgtctgct agcaggatct
     1021 tcaacatgtc atccttcttt cgccaccgga atggctccga cactcactga aacagtggtt
     1081 ccttgttgga cggcgctctc gattgcgatg cgccctccga tcacgtcgac gcgttcgcgc
     1141 atgccgacca ggccgtacgc actgtcgcgg acccccttgg cggcgtcgaa gccccgtccg
     1201 ttgtctgcga tgaccaggct ggcggtcccg gcatcgactg cccacgtgac gtcgacccgg
     1261 ctcgccttcg cgtgcttgtc gacgttgttc aatgcctctt ggaggatacg cagcagctcg
     1321 ttctcgaccg ggaccggcat cttctccgtc gggttcgtga ccctgaacgt cacctcgagc
     1381 ccggtgcgcc cggcgtaccg gtcgacgagt tccttgccca ccatcgagag tgggcgatcc
     1441 tcggtgatgc cactgcggag ctgacgcaac gtctcgcgca actcgccgag cgccgtctgc
     1501 acgtccgcgt acagctgggt gagttgcacg aatccgctgc cgtcggtcga gatgaagcgc
     1561 tcgagttcga aactgatgta ggtgagccac tgcccgagtc ggtcgtgcag gtcgcgggcg
     1621 atgcgggcgc gctcaccctc ggccccgagc gatcggaggc ggccgaacca gcgggcgttg
     1681 tcgagcgtga gcgccacgac gtcggccata cccaagagca gacgacggtc gcgatcgcgg
     1741 tactgaccgg gggttgcgtg ttcgatgccg agcacaccca cgacccggcc gcgtgactcg
     1801 agcttcgtgt agagccccga ccccgacgtg ggagagatgc cgggaagcga cccggcggtg
     1861 aggtcgggaa cgagccacgg ctcgcggctg tccatcgcct ggcgcaggtg caccggcaac
     1921 tcgtcgaccg acgacgtggg cttgaccgca cacccctcgg tcagcttcgg gatccactcg
     1981 tcgtgcgcct cgtcgtactc gaggaggcag atgacgttgc tgcggaacgt gtcgatgacc
     2041 tgctgacggg cggcgtcgat cgcctcgcgc tggttgaggc tcgtcggcag ggtgcgggca
     2101 acggcattga gcatcgtgag caggtcgttg gtctcggcga gtgtgtcgac ctggccggcg
     2161 agccgacggc gacgttcctc ggcctcgatc agtcgcgtgc ggaggaaggc ggaaccgagc
     2221 gccaccaaca ccatggagcc gaggagcagg cccaccgccc ggtcgagggt gaccgtctcg
     2281 ccggccggat ggatggcgtg accgacgatc atggccgccc agccgatgac gaacgcgagg
     2341 gcgccgtcga ggtaccccca accgaacgcg gcgacggcga tgccggccat cacgcagaac
     2401 acgaacggcg acaccggccc accggaggcg ccgacggcca ggcccaagat gagcacatcg
     2461 gtcagcgcga ccacacgatc gagccggacc cgcgatccga gccggatcgg gatcatggtg
     2521 cgccaacacg tgaggaacag gcagaccgac aacgtggcga cgacgctgta ggagcccttg
     2581 aacgccgcgg cggcaccgaa aatcatgccg tacatgacgg cggcccaccg gaacgcgttg
     2641 atgaccgggg agatggtggc ctggatctgc ggggagacgt cgaggttgcg ggccgtctcg
     2701 tcgacgccgc cgaagggaag tgcccgaagt gcggccacca tgtcgaccga gctcgggacg
     2761 ggcgcggtgg cgcgcgaacg cgcgccgaac tcgttcgggc tcgacggact ggtggccgac
     2821 gggttcagaa tcgacttccc gtgctcgggc atggcttcct cgcaggtccc gccgctggtg
     2881 gcggaggaac acagggttgt cccgcacggg cgaaccctgc ggaaatttgg cactcaacgg
     2941 gcgagggagc ccgctttcat actatctgtt gtacggcgcg accgtgcgct gggtagtcct
     3001 cggcgggcgt gggtcatcag gcccgggccg atcgacctag attggccgtg tgaccacgag
     3061 cgaatccgat gaatcgtcgg ttcgtgccgg cgctctcgga acggggatcg tgacagacga
     3121 catcgaggtc gtccactatc cgggggcgaa gcggcccgac cgccgcgaga ccctcgacgc
     3181 agccggcgtg gcgatctcca aggtggagtg gggcgacccg tcggcgcccc cgctgctgtt
     3241 cgcccacggc ggcttcgact tcgccgagac cctcaatgtg ttcgccccac tgctcgtggc
     3301 ccaagggtgg cgcgtcgtca gttgggacca tcgaggccac gggcactcgg catgggccga
     3361 cctctacagc tgggaggccg acctccgcga tgccgcgacg gtgctcgagt cgatcgggcc
     3421 cgctccactt ccgatgttgg gccattccaa agggggcgca ctcacccttc agttggccga
     3481 ggcatttccc catcggttca cacatctcgt gaacttggac ggccttccgt cgcgccgctc
     3541 catgcccgac atcgcggagc acgaacgcac gagaatgctg gcgcaggagc tgggtgcttg
     3601 gctcgatcac cgtcggcgtg tctcggacaa acagcgtcgt ccggggacga tcgacgagct
     3661 cgccacccgg cgggggcgga tgaacacccg gctcgatcac gactggctgc gctacctcgt
     3721 caccgtcggt ggtcgtcacg acggcgacgg ctggcgctgg cgcatcgacc ccacccttcg
     3781 gatgggcgga ttcggcccgt ggcggccgga atggtcgatg ttgcggctcc ccggcctgtc
     3841 catgccgttc ctggcagtgc tcggcctcga gctcgaggtg atggggtggg gcacgcgccc
     3901 caacgacgtt ctcccctacc tcccgcccgg cgcacacttc gagcagctcg aaggtatcgg
     3961 ccacttcatg cacatcgagc agccccaact catcgccgac atggtgatcg atttcgtcgg
     4021 tgcgccggaa tgagcggcac cgacagcgag cctacggcct gggtccggca cggccggagc
     4081 gacctggcgc tccagtgcct ccgcgagggc gagggccggg cgctcctcct cttgcacggg
     4141 ctcggtgagc gcgcgccgac cgtcgcaccc gactgggtcg ctccgtggcc cggtcccgtg
     4201 tggggcctcg acttcaccgg gcatggccgc tcgacccttc cccgcggtgg cggatacacc
     4261 gcggagatgc tcctcggcga cgctgatgcg gcgctcgagc acctcggcga ggccaccgtg
     4321 gtcggacgcg gtctgggcgg gtacgtcggc ctcctgctcg ccggcgcccg tgccgcacgg
     4381 gtacacggcg tgatcatctg cgacggtccg ggcatcgccg gaggcgggat ccagccgggc
     4441 tcaccgttcg tcgtgttccc ccggttcggt cacggcgaga cccccgaccc gttcgccatg
     4501 gtcgagttgg cccgcgatgt ccggcctccg gactacgccc tgaactacgt gcggttcgcg
     4561 ttcgagtcgg ccccggtgcc cgacccggtg gtcgtcgccg ccgtcgtccg ccccgaatgg
     4621 ctctccgccg tggccgtcga gcccggagtg atcgagtgcg aacggtccga ggcgctggcg
     4681 tccttcatcg actgagctga acggcttatt tgtggtcggg gagcgcccca acccaacagt
     4741 tgccggtcgt tcacgtcgaa acagatgggg tgagcgaagg cctcgcgggc ggcgattccg
     4801 tcctcgaacc cgaccgggac gacgccatcg gcgtcatgag tgccgagttc gtcgaccccg
     4861 cactcgagcg tgagttccag ctgcatcacc tcgactcgac cgcccgccgg gccatctcca
     4921 cctccgtggt cggtgcatcg agctgcttcg tgttcaccgt gtcggacttc acccggctcg
     4981 ggttcggttc ccacttcgtc ctgttgctcg ccctccgctc cttggtcctg ctggcctcgg
     5041 tcctcttcgc catggccgtc cgccggaatc cggtccgcag cctttcctgg aagttcgtgt
     5101 cgaccctcga gtcggccgtc ctcgcggtgc tcttcgtggt catggcgttc cggcccgagg
     5161 aaaccgaggt caacaacctc gggatctcgg tgaccatcgt cgcgatgttc ctgctcgtgc
     5221 cgaatcgctt tccggccgcg ttcgccatct ccgtcgccac cgtcgtcatc gatctcggcc
     5281 tcgccgtcac gttggtcgac agcccgctga gtcgtcagat tccctcggcg ttgaccctcg
     5341 tgtcgttcct cgtcatcggg gccatgagcg ccaaccagct ccaacgcgcc cggcgggagg
     5401 agttcctgtc gttgcgtaac gcccggacct cgaacgacct cctcgtcgag gagatcggac
     5461 gacgacgcgt cctcgaggac gagctgacct ggatggcgac ccacgatgcg ctcaccgacc
     5521 tgctcaaccg tcgagccttc tacgaactcg ccgagcgtga agcggtgcgg gcgcgccgca
     5581 ccaacctccc cgtttcgatc ctcgtgatcg acgcagacga gttcaaggcg gtcaacgacc
     5641 ggttcggtca ccacgccggc gacgaagcgc tccgccgcat cgccgccacg tgcaggcaac
     5701 acctccgcgc cgacgacgtc gtcgggcgag tcggtggtga ggaattcgcc gtcgtcatgc
     5761 ccggcgcgaa cagcgcgctc gcacaccatg tcgccgaacg tctccgggtt gccgtgaccg
     5821 aggcccccat cgatcacccc gacggcccga tcaacctgag cgtcagcatc ggagtcaccg
     5881 aataccgcgt gtggcacgag gccatgctcg acgcaatcca acgcgccgac gaggcgatgt
     5941 acctggccaa gaccaccggc cggaactgcg tcgttcccgt ctgatcgatc aggcgggtgg
     6001 cagggcgagg ccccggaagg tcgcgcccag cacggcctcc tgcaggtccc ggcgggcgtc
     6061 ggcatcggcg gagacgccca ggaggagcac ctgcgccaac ccgtgggtca ccgtccacag
     6121 cgtcagcgcg gtgacgaacg ggtcgtgatc ggggtggatc gcaccggtcg cgatcgcgtc
     6181 ggcgatggcc ttggtcggtt gttcgaacgc ttcggtcgcg gcggcgagtt ccgtacgggg
     6241 gttcggcagc tcgagcgcct ggggcgggaa catgaacatc gcctggaaca gccccggatt
     6301 ggccagggcc atgtccacgt agatgaggca ctgctggcga atccggtcga tcgggtcgtc
     6361 ggtggcgatc gccgcgtacc gatcgagcag ctcacggaag cccgtctccg cgagcgtggc
     6421 gaggaggtcg gacttgtcgc ggacgtgggc gtagagcgcc ggtgccgtca cgccgaggtc
     6481 actcgccaac cgacggagcg acaacgcgtc cagcccgtgc tcggacacga gttgcaacgc
     6541 cgccgccacg atggcgggac gactgagggc ggtgcgggct gcggggggtt gctcgggacc
     6601 ggtactcacc acgtcggatc gtaggagatc aggcgggcgg cgtcggcttc cgagagtggc
     6661 ttggagatga ggaagccctg agcgacgtca cagccgagcg accgcagctc ggcgaggtcg
     6721 gactcggtct cgactccttc ggcgatgcac tgcaacccga gcgtgctcgc gaggtcgatg
     6781 acggatcgga cgatcgccgc gttgtcgcga tcgttgccga tcccgtcgac gaagctccgg
     6841 tcgatcttga ccacatcgat cgggaacgag cggaggtagg tgagcgacga gtagccggtg
     6901 ccgaagtcgt cgagcgacag cttgagaccg agcgccttca acttctcgag gaccatgatc
     6961 gaccggtcga cgtcgtcgag gaggatcgac tcggtgatct ccatctcgac gttgttggga
     7021 ttgactccgg tctcctcgag gatcgcctcg atgtcggcga tgagcgacgg cttcacgagc
     7081 tgcttgcccg acaggttgat gcccatcgtg aagttctccc cgccggggag ctccgtcgac
     7141 cacttggcca gggtacggag ggcctcttcg atgacccacg agccgatcgg gatgatgagc
     7201 cccgactgct cggcgatcgg cacgaagtcg ctcggcgaca acaggccgtg ctcaggatgc
     7261 tgccaccgga tcagggcctc ggcgccgttg atgcggcccg tgtcgagcga gatctgcggt
     7321 tggtagaaga gccggagctc gttccggcga agcgcacgcc ggagcccggt ctcgacagtg
     7381 aggcggtcga cggcccgggc gcgcaggttg ccgttgaaca cctcggtgcg gtcgcggccc
     7441 cggagcttgg cctcgtgcag cgcggcattg gcgtcgcgga tcaccgacgc cggatcacca
     7501 cgggttccgt cggtcaccgc gaccccggcg gtgaacgtca tcaggagttc gacctcggcc
     7561 atgccgacgc gacccgacac cgccttgcgg atgcgttcgg ccaatgcctc ggcaccctcg
     7621 gccgtctcca ggccctccat cacgatcgcg aactcgtcgg cgccgaagcg ggccaccatg
     7681 tcgcgcgacc agaccaggcc cgtgagcttc tgagccgtct gcttcagcag gttgtcgccg
     7741 acgcggtgcc cgaggctctc gttgatgatc cggaagttgt cgaggtcgag gaagacgacg
     7801 gcgacccacg tgccgtcctg cgcgcctcga tccatcgcct cggcgaggcg ctgggtgagc
     7861 agggcgcggt tgggcagatc ggtcaggggg tcgtgggtgg cctcccactc caaccgctcc
     7921 tcgaactgct tgcgttggtt gatgtcgcgg agcacggccg aaatgcggtt gagctgtccc
     7981 gatttctggt ggtccgggat gacctgagcc gagaagggct cccacgtgcc ggcggcggac
     8041 cgaagggtca cctcgccgtg ccagatctcc cggctcgcca tgtgctgagc gatcgtctcg
     8101 agggcgtcgg cgggccgcgt ctggaagtcg agcggcgtcg tcacggtcgc caggagggct
     8161 tcctgctcga taccgaggaa ccgacgaccg gccgggttca catacagcgg catgagttcg
     8221 gggtccatca cgatgaccag gtcgctcgac gcctccatca gcgtgtagag acgggcctgg
     8281 tcctcctcgg cgcggacccg agccgtgatg tcctccaccg cccacaggta gttcgtgatc
     8341 tcgccggcgc tgtcgagctg gggaaggatc cgcaccgaac cccaccggag ctcgccgtcg
     8401 ggccggatga accgcatctg cgtggtggcc gaatcgttct cgcccgccgt ccgccaggcc
     8461 gccagcgcgt tgtcgatgtc gccctcgtgg atgtgtcgac cgagctcgtc acggaggaga
     8521 tagttggccg acgtccccat gatctgcttg agccgggcgt tcaggaacac gaagttgccg
     8581 tctcggtcct ggaggccgac accgaacggc gcttcgttga ctatcgtccg gaaccgctcc
     8641 tcgctcgtgc gcagcgcgcg ggttccggct acgcgccgcg aggcgtcgcg gatcgtcacg
     8701 agcacctggg gctcacccga ctggtcgatc gccagcacgt cgacatcgca ccacaccgac
     8761 gccgagtcgc caccgaacac ctgacaacac accggctcgg agtcgtcgac gcgagcggca
     8821 ccctgcaccc gggcgcggat cgccggccgg ttcgtgtcgt cgatcaggtt gccgatgtcg
     8881 acaccggtca ggtcccgggg ctggtgtccg aggatcgagt acaccgacgg ggacgcccac
     8941 tggatcgcac catcaggcgt gcacaggagt gtcgccaacg cggcattcgt cagatatccc
     9001 cacaacagct gggaatcggt gatcgcagct gcgagttcgg tcgggcgttc gtcgaggacg
     9061 gcctgactca tggggggatc acgatcgggc ataccgcttt ccatcggcac gttgccgtgg
     9121 aactgaaacg ggcgtgaccg aaaatccgtc ggtctcgtga ttcgggtcga tccggggtca
     9181 ggatccggtg gtctcgtcga ggtcggcgac gatctcggtg acccggagtt tggcggtgtc
     9241 gagccgttcg cggcacagac cgacgaggaa cgcggcacgg gccacgtggt cggccaagtg
     9301 gtcgatatcg agccggtcgt cctcgagttg ggtgaggatc gactcgagtt cggccatggc
     9361 cgcggcgtat ccgggggtct cgtcggtcat gtgcccggaa ccgtagcgcc gtcggaggcg
     9421 ataccggacg acgacgccgt gacgaccgac gtgatccgtc catcggcgac ctgggtgacg
     9481 agcacgtcgc cctcgtcgag atcggcggcg cggacgacgc ggccgtcggc tccccgcgtg
     9541 atcgaccaac cccgcgccag cgacaccgcc gggtcgtagg cgcggacggt ggccgcgatc
     9601 gcgtcgagcc gccggccctg atcgccgatc gcccgcgggg ctgcagcgac gaggcggcgg
     9661 cgggcgtcgt cggtgcgggc ggacccgcgg atgacatgcg catcggcgag gcggcgcacc
     9721 cgatgcgccg ctccggcgag gtgcgacgag ctgcgatcga gatcggcgag cgcggtgcgg
     9781 gccgtgcgac tcgccagccg atcgagccgg atcgcgctcc gttccagacg ccgcgccgtg
     9841 gcgtcctcga tgccgctcca caggtcgtcg agctgctggg caccggcgac gacgagctcg
     9901 acgacggctg ccgcgcatgc cgtcggcgtc ttccacgagc ggtgggcgac ctcgtcggcg
     9961 acggtccggt cgatctcgtg gccgagcccg gtccagaccg ggacgggcga cgacgcgacg
    10021 gcgcgggcga cgatctcctc gtcgaaggcc gcgagatcgg tgcgggcacc gccaccgcgg
    10081 accagtgcga tgaggtcgac accggcggca gcggcgcgcc cgagtgccgc aacgatcgag
    10141 gcgggagcat cggcgccctg cacgcgggtg tcgaccacga ccaggtcgaa cccgatgcca
    10201 cccctgccga gctcgtgcat cgcgtccgca tgcgcagcgc tgccgatgct cgtgacgact
    10261 gcgatgcgga gcggcaccgg ttccaggggc agtgccgcgt tggcacgcag gagcccctcg
    10321 ccgtcgaggc gggccaacag gagggcccgc tccgccgcca tgttcccgag cgtgaacact
    10381 gggtcgatgc cgctcatctt gagctgcagt tggctccgcg ctccgtagac ctcgacgctc
    10441 ccgcggatgc ggacccgcac gccgtcggag atgcggacgg cgccgccgct gcgacggaga
    10501 tggcggttga cgttctgtcg gtgccactcg aacagcgtga ccgccaacgt gttcggggcc
    10561 cgccccccgg cctcgtcggc tggctcggtc aacgtgaagt agacgtgccc ggcgttggag
    10621 cgttgcaggt tggagatctc gccctcgatc cagatctcgc cgccgagcgt ctcgccgatg
    10681 acggcgttga cccagtcggt cagctgggcg atcgtccacg tcacgacgtc aggcacctgg
    10741 cgagggtagt tccgtccggt ggcctcgtcc ctcgcccgac ggtccatact tctggggatg
    10801 gcggaagcga acgaggggaa cgagacgtcg ggcgcccgac gttggtcccg ccagagctcc
    10861 ggcaccgctc gtgcgcgttc gaccgtgcgc ggcgtgctcg ggttccagct cctcgccaat
    10921 ggcctcggcc tgtcggtgat cctcctctac gtcacgctgc tcttcccgcc cggcaacgac
    10981 gaacaggaga gcgcgctcaa cctcaaggtc ttcggggcgt atctcgcggt gaacctcctc
    11041 gtcggcctcc cggtcaacca gctcctgttg cggcgtgcgg tggtgtgggt ccgagaggga
    11101 accgaggcga cggcccggca acggtggctg gtccttcgac tgccgttgct cgagaccggc
    11161 acggcgctcg tgtcgtggtt cggtggagcg atcctgttcg ggatcatcaa cgagtcggcg
    11221 cgacgcgtct cggtcggcat cgccctcgcc ggcgtcgtca cctgcacgct gctgtacctc
    11281 ctgttggagg gccacttccg gccggtcttc gccctggcgc tccgaaaggc cgacctgccc
    11341 gaagggcgcc gcgatgtctg gccccgcctg atgctcgcgt ggctgctcgg cagtggcatg
    11401 cccatcgcgt tgttgggtgc cgccccactc ctggacgaac gcttcgactc gtcgcgactc
    11461 ccgtggatcg ccgcgttcgg gttgctcggc ggtggtgccg tcatgacgac cgctgcgatt
    11521 tcggtgtcgc gccccctcaa ccgtctccgc ggcgtgctgc gtgacgtcga gcagggcgaa
    11581 ctcgacatcg acgttcctgt cgacgacctc ggagaactcg gccgactcgc cgagggcatc
    11641 aacgacctgg tcgccggact ccgcgagcgg gagcagctgc gggacgtgtt cggacgccag
    11701 gtcggccaac cggcgctggc gcacctgccc ggcgacacgg agagcttcga cgccgccggg
    11761 gaacagcgcg acgtgaccgt gctcttcgtc gacctccagg gctatacccg gttcagcgag
    11821 tcggcatcgc ccgaggcggt cgtcgcgatg ctcaaccggt tcttccgcgt ggtcgtcgcc
    11881 gcggtcaacc gtgagggcgg ttgggtgaac aagttcgagg gcgacgcagc gctgtgcgtg
    11941 ttcggggcgc cgcaacccga cgagtcgcac gccgcgaacg cactgcgcgc cgccgaagcg
    12001 ctgcgccgcg acctcgcccg tggcgaacac gaactcctcg ccggcatcgg cgtcgccacg
    12061 ggcaccgccg tggccggctt catcggcacg cccgaacgtt acgagtacac ggtcatcggt
    12121 aactgcgtga acctcgcctc gcggctgtgc gatctcgcca aggaggaacg ctccggggtc
    12181 ctcgcgtccc tcgacacgtg ggtcgccgcc ggccgccccg aaggatggct cgatgtgggc
    12241 cgggtccggg tgcggggccg gtcccagaaa ctgacggccg tcaccctggc gtcagggcga
    12301 cggtcccgcc gcgagacgtc gggacggttc aggccgtcgg accggtgagc gtccggtaga
    12361 gatcggtgta gagcccgccg gcggtgacca gcgtggcgtg ggtgccgcgc tggacgatgc
    12421 gcccggagtc catgacgagg atctggtcgg cggcggtgat cgtcgagagt cggtgggcga
    12481 tcacgatcga ggtgcggtcg gcgagcgccg tggccagggc catctgcacg aggtgctcgt
    12541 tctcgctgtc gaggtggctc gtcgcctcgt cgaggatcac gatcgccggg tccttcacca
    12601 gcattcgagc gatcgcgagt cgttgcttct caccgcccga caggcggtag ccccgctcac
    12661 ccaccaccgt gtcgaagcca tcggggaggc gctcgatgac gtcgagtacc tgcgccgcct
    12721 tgcacgcgtc gcgcaactcg gaatcggtcg cgtcgggccg ggcgtaccgc aggttctcgc
    12781 ccacggtggt gtggaacatg tgggggtcct gggcgacgat gccgatcgag ctccgcaggc
    12841 tcgcgaggga gatgtcgcgc acgtcggtgc cgtcgacgcg gatcgctccc gagctgacgt
    12901 cgtacaaccg ggtgaccaac gacgacatcg tcgactttcc ggcacccgac gggcccacca
    12961 acgcgaccat ctgcccgggc tcgatcgtcg ccgtgacgcc gtgcaggacc gtggccagtt
    13021 cgtcggtctg cggcgcgaag ttgccttcga gggacgccac cgacgtgtcg gacgcggcgg
    13081 ggtagtggaa cgtgacgtcg tcgaactcga tgcggccctt ggcccggccg agatcgacgg
    13141 gctgcgcggc atcctggatg ggtgtgggcg tgtcgagcac ctcgaagacc cgctcgaagc
    13201 tgacgaaggc cgacatcacg tcgatgcggg cgttggtcag cgacgtcaac ggctcgtaga
    13261 tgcggacgac caacgccgcg agcgccgtga gcgttcccag ggtgatcacg ccgttgatga
    13321 cctggcgtcc gccccaccag tagatggcag cggtacccat ggcgcccacg aggcccatca
    13381 ccgacaggaa gatgcgggtg agcatcgccg acttcacgcc gatgtcgcgg accttcgcgg
    13441 cacggcccga gaactcggtc agctctcgct gctcgttgcc gtacgtcttg accaacatcg
    13501 cgcccgacac gccgaaccgc tcggtcatga tgttgttcat ctcggcgttc tcctgcatgc
    13561 cgtcacgggt gatggtggcg agccgcttgc cgacccgctt ggcgggccag atgaacaacg
    13621 ggaggaggag caccgacgcg atggtgagtc gccactcgag ccgcgccatc gccgcgagcg
    13681 tgacgaccac ggtgatgacg ttcgacacca ccgtgccgag cgtgccggtg aacgcccgct
    13741 gggcgccgat gacgtcgttg ttgaggcggc tgacgagtga accggtctgc gtgcgggtga
    13801 agaaggccat cgggaggcgc tgcacgtggt cgaacagctc ggaccggagg tcgtagatga
    13861 ggccctcacc gatgcgggcc gaccagtagc gctccgcgag gcccaacagg ctgactccga
    13921 cggcggcagc gacgaccagc gccgcgagct gggtgaggta gccggtgtcc ttgtcggcga
    13981 tggcgttgtc gacgatcgcc ttgaacagga ggggcggggc cagcgacagc agcgcctcga
    14041 ggacgatggt gaccaggaag ccgatgagca tgccctggta gggcttcgcg tagtgcccga
    14101 cgcggcgaac cagcccgcgg tcgagcttgc gtttcgcagc ttcctgatcg ctgtacgtca
    14161 ttcggtagag cacgtgcacg tcggactcca acgctttgat gcgtgaacat cattccaaca
    14221 cggcgcgggc cgaccatgat cgaggagggg agtaaggccc tcgagccgac gagcccgagg
    14281 gccgtaaccc tgcggagagt aagggatttg aacccttgga ggcttgcacc tcaacggttt
    14341 tcgagaccgt cccgttcgtc cgctccggca actctccggg atcgaggtta gcggaggacg
    14401 gggtgacccc gtgacgagtt cagatctcgg tcgtccggcg ggatgcgaag aacgtggtga
    14461 ggagcgaggc gcactcctcc ccgaggaccc cggcccgggt cgggaactcg tgattgaggc
    14521 gagggtcgct gccgaggtgg tagagcgaac cggtggcacc cgccttcatg tcgacggccc
    14581 cgaagaccac cccaccgatg cgcgaggccc acatggcacc ggcgcacatg gggcacggtt
    14641 cgagtgtgat gaccacggtg acctcgccga ggcgccactc gccgacgacc gccgcggcct
    14701 cccgcagaac gaggagttcg gcgtgcgcgg tggggtcctg gtcgatcgcc cggcgattac
    14761 cggcggttgc gatcacgtcg ccgtcgcgga cggcgacggc accgatggga agatcgccgg
    14821 cggcctcggc ggcccgggcc gcgtcgatgg cgaggcgcat gaaacgttcg gggtcggcgg
    14881 cgacagcggc gtcgatcggc atcggcgcag cgtacgcgac gggttctgtc ccgcgagggg
    14941 acgcgatctg tgtcccctcg tgggacagaa cctatgcggc gtggatcagc ggctccggcg
    15001 acggcggacc gccaggacga gcgccccggc gaccatcgcc agcccggcgg cgaggagcag
    15061 caccgacacc gtcgtgccgg tcgtggcgag gggtgatccg gtgctcggcc gcgtctcgac
    15121 cgcgcggcgc accgccggcg gcgacgtcgt cgtcgtcgtg gtcgtgggga cctgcacctc
    15181 ggactcgacg acggtgaccg ggttcgtcac cgacgaggtg ttgttcgtgg catcgcgatc
    15241 cacctgcgtg ctcccgacgg tgcccgtgtt ggtgatcgtc ccccgggctc cggccgccac
    15301 ggcgacggtc agggcgatgg cggggagcgt ggtgccgctc gccagtgtgg ccgcggtcga
    15361 gcaggtgacg gtcgtcccgt ccagggcgca cgtccacccg ctgcccgtgg ccgtgagcgg
    15421 ctgcagcccg gcgggcaagg tgtcggtgac cttgatcgaa ctcgccgtcg ccggaccctt
    15481 gttccgcacg gtcaaggagt acgtcgcctc ctgccctgcc acgagcgacg acgaggtcag
    15541 cgccttgacg atggcgaggt cgaccgcggg atcgggcgtc gggacggtcg cagcgtcgga
    15601 gacgtcgggc gtctcgtccg agctcgccac cgccgtgttc acgtaggaac cggcgttgaa
    15661 gtcggcctgc gtgaccacgt gcgtggcggc gcacgtgagc ttctcgccgg acgccagggt
    15721 gacgcccggg cccgacggtg cggcggcgca gccgctcgac agcacggctc cgacaccggc
    15781 ctcctcgatc cggaccccgt tgagggtcgc gacgccggtg ttggtcacgg tgatggcata
    15841 ggtgatcgtg tcgccgaggg cgtaggggcc ggtcgacgtg gccaccttgt cgatcgacag
    15901 ggccgcccgc gggatcgacc acgtgtcgat gaccggtggg gtctgatcac tgtcggccgt
    15961 cgccacgttg tccacactgt cctcggtcag gtcggcggcc gacacggcgt cggcagcggt
    16021 gcacgtgacc gacacgccgg ccgccatggc gaacggctga ccgttgccgg gaccgtcccc
    16081 gttgcagtcg atcgacgcga ggggaatccg gtcgtcggcg acggtcacgc cggtcagagc
    16141 cgccccgccg acattggtca cgacgtaggt gtacgtgagc acgtcaccgg ccgaagcgta
    16201 cgagccgggg tcgcccgact tctcgatgcg caggcgggct acggggatgt cggcgtcgtc
    16261 accgggcgag gtcgggtcgt cgggtgacgt gacggtggcc tcgttgtgca cgaccatcag
    16321 tgacgggctg gtccccacgg cgtagctgat cgtgaacgtc accgacgtcc cgggcgcgat
    16381 gtcgcccagc gagcagctgg tgccggtctc gccgcacaca ccaccctgcg atgccaggta
    16441 gtcgacgccg gcgggaagcg ggtcgttcac gattgcgtcg tgcgcggtgc ccccgccggc
    16501 ggcgttggtc accgtgatcg agaaggtcgc cacgcctccg ggcagcacgg tcgacgagcc
    16561 cggtgatttg gtgatcgtca gatcggccgc cttccgggtg ttggtgaagg tgcacacgac
    16621 ggcggcgtcg gcggatacct cgacgttgcc cgcttccacg tcgaccgtcg acccctcgtc
    16681 ggcggtgtcg atgcaggcga cggtggcgga gtactcaccg ccgggggtcg acccggcggc
    16741 cgcttcggcg atcgcgtgga tgcccggcgc cacggtcacg gcgccggtgg tgccgttgtg
    16801 ggatgccgcc gccgtgcgga cggtgccgtc gacggacagg tcgaaggtgc cggggtcgtc
    16861 ggtcggggag agttgcttgc gcacttcgag ggtgccggtg cggcgggtgt tcgtaaacgt
    16921 gcagacgacc gctccgctgc cgtcggccat gatgtcgagg ctggcgccga agccgtaggc
    16981 gatcggggtc cccccggcgg tacaggcgat cgccgagtcg tagccgtcga gatcggcgcc
    17041 ggtctcggag accgtgacgg aaccggtgtc gacggtcgtc gtcccgctcg aaccgtcgac
    17101 ggcgtcgtcc acgatcacgg tgtcgtcgac caggaggtcg aaggtgtgac cgctctcgag
    17161 cggatcgacg acgaccttct cgacggtgat gccgacggtc gcccgggtgt tggtgaacgt
    17221 gcacaccacc acgtcaccgc tcgcgacggc gaccgcgccg ttggtgacga ccagcggcgc
    17281 gttggtcacg gtctcgcggc aggcgagcgt ggggaggtag tcgagggagg cgttggtggg
    17341 ggagcccttc tccgccacgg cgtggacgcc ggggatgacc acgatcgcgc cggtcgtgcc
    17401 gttgtgccct gctccgaccg tgcggacggt gccgtcgatc gagaggtcga aggtggccgg
    17461 atcgttggcc ggtaccagct gcttgcgcac ctcgatggtg ccggtgcgtc gcgtgttggt
    17521 gaacgtgcag acgacattgg ctccgacctc gacgtcgatc tggagcgagc tcgcgtcgtt
    17581 ggccctcgca accggttcgc cgtcctcggt gcagacgatc gaggtggcgt actgcccgag
    17641 gttcgctccg ctctccgaca ccgtgacggg gccggtattg accgtcgtcg accccgtcga
    17701 gccgttgacg gcctcgtcga cgaccacctt cgccccggcg aggaggtcga aggtgtgact
    17761 gctcgccagc gggtcgacca caaccttggt gagcgtgaga ttgcccgtcg tccgggtgtt
    17821 ggtgaacgtg cagacgaccg cgtcgccggc gtcgacctgc acggacccgg cggtggggat
    17881 caccggcgca ttgccgttgg cgctgtcgac gcagctgagc gatgccgcgt agtcgccgag
    17941 cttcgtgtct gctgccccgg cctcggcgac cgagtgcggg ccctgggcga ccgtgacggc
    18001 cccggtggtg ccgttgtgcc ctgctccggc cgtgcggacg gtgccgtcga tcgagaggtc
    18061 gaaggtgccc ggatcgttgg tcggtacgag ctgcttgcgc acctcgagcg tgcccgtgcg
    18121 tcgggtgttc gtgaacgtgc acacgacgtt cgacccggca gacacctcga ccgtgagtga
    18181 cgttcctcca cccgaggcga ggggttggcc acctgacgtg caggcgatcg ccgtggtgta
    18241 ccgcccgagg gcgccactgg tctccgacac cgtcacgggt ccgctgttga cggtggttgt
    18301 gccggtcgag ccgtcgaccg cttcgtcgac gacgacgtcg gcgccggcga ggaggtcgaa
    18361 cgtgtggttg ctgccgaccg ggtcgacgac gacctttcgc agtgtgagcc caccggtgga
    18421 gcgcgtgttg gtgaaggtgc agacgaccgc gtcaccgggg tcgacctgga ccgtgccgtc
    18481 ggagacggcg actgcggtgc tgccgtgggc ggtgtcgatg cagctgaggg cggcggcgta
    18541 gtcggcgagg ctcgtgccac ttgccccggc cccggcctcg gcgacggagt gggtgccggc
    18601 ggcgaccacg accggaccgg tcgtgccgtt gtgtcctgct ccggccgtgc ggacggtgcc
    18661 gtcgatcgaa aggtcgaagg tcccgggatc gttcgggggc gagagctgct tgcgcacctc
    18721 gagggtgccc gtcgaccggg tgttggtgaa cgtgcagacg atgtccgctc ctgcggtgac
    18781 gttgacggtg agcgacgtcc ccgtccccgc ggcgatcggc tgcccggcgg agttgcacac
    18841 gatggccgac gagtagaggc cgaggtcgcc tccggcctcc gacacggtga cgggtcccgt
    18901 gttgaccgtg gtcgagccgg tcgatccgtt ggtcgcgccg gcgacgacgg tggtgccgcc
    18961 ggcgcgcagg tcgaaggagt ggccgctgtt caggggatcg acgacggtct tggtgagcgt
    19021 caggttgccc gtcttacggg tgttggtgaa cgtgcagacg accttggcgc cctcgtccac
    19081 gaccacgttg tcgtccttgg tcacgaggac cggcgtggtg cccgccgtgt ccacgcacgc
    19141 gactgtcgtc gtgtagtcgg acaggttcgc cgcgtttccg gcggccaccg tctcggcgat
    19201 cgagtgtgaa ccgaccggaa cggtgcgctc accggtcgtg ccgtcgtcgc cgaccccggc
    19261 gacctcgtgc ggcccgccgt cgatgtcgag gtcgaaccga cccgggtccg tcgacggcac
    19321 gagttccttg cgcagttcga gcgttccggt cttgcgcgtg ttggtgaagg tcacggtgtt
    19381 cgtgccgtcg gcgatggtga ccgctccgcc ggccggagcg acggcgctcg agtagccggc
    19441 gttcgccgtc tccgacaccg tgcacgccgt cccggtcggg atccccgaca cggtgtgctg
    19501 ctggcctgcc ttgagtccga tcgtgcgggg ctggggaccg cagtcccact ggaacgtgaa
    19561 cacctggtcg gggtcgccga ggctgttggg cgggaacacc ttcccgatga ggaggtcacc
    19621 gacccgacgg tgatcgacgt agctcgccgt ctgggccgcg ccggtcgacg ggaagccgac
    19681 cgtgccgttg gcagccggcg tcacctggaa gaagtcgccg ctcacgctgc cgttcgggtt
    19741 ctcgtccacg acgcagttcg atccggtcgg aacgttcgac accgtcaccg agccggcggt
    19801 cgagggcgcg ttgatcgtga tgtcgaacgg gctgcccggc accggggcac cgccgcaggt
    19861 gaggtcgaag cggaaggtgc ccgcaccctg atcgatcgtc ttggagatcg tgatggtccc
    19921 gacgtcgcgt cggttcgaga aggtcacgtt gttgccctgc gaggccacgg tgatctgtcg
    19981 aggagtgacg ttgccggcga ccgagccgtc gagggtccac ccggcgggga ccgcttcggt
    20041 gatcgtgcac gatgtgccca ccggcacgcc ggcgatcgtg gccgtgccgt tggcgctgtt
    20101 cgacgccgtg atcgtccggc tgaaggtacc tgcggcccca cagtcggcga cgaagtcgaa
    20161 cgaaccggac acgggcagca cggaggtgcc ggtcacggcc ttcgtgatga cgaggtcggc
    20221 ggtctgacgg gtgttcgtga aggacgcggt cgcctgctgg ccggcgacga tggtgatcgg
    20281 tccggcgggt gccgccgtct gggtccagcc cgccggcacc gtctcggtga cggtgcagtt
    20341 caggccggac gggatcccgg tgaccgcggt cgacgttccg gttccacccg aggtcgtgac
    20401 cgagcgtgtc acgtcccacg ccgtgccgtc gcaggcgacg gtgaagtcga acgttccgtc
    20461 gccgccgacg gtcgatttgt tcacgatcag cgacccggtc tggcgggtgt tggtgaacga
    20521 cacgctctcc gcggcctggt cgaccggctg cacgctctgc gagccactct gcaaccaccc
    20581 cggagtcgtt gcctcggtca ccgtgcagct ggcgccgaac gggatgtcgg acggcgtctc
    20641 ggccgtaccg ttcccgctga tgttcaaggt cttcgggtac ctggcgacgc ccggacaggc
    20701 gacggtgaac tcgaacgtgg gtgagccgga gggatcgccg gcgacggcgt tcgtcgtctt
    20761 gttcacgacg accttggcga agcgacgggt attggtgaac gccacgttgc ggtcctggtt
    20821 catcgtggtg ctgccaccgt tggtgacgat ggcgccggtc accgtcaccg cgaagttcgc
    20881 gttggtggcc tcggtcaccg tgcacgacgc tccgggcgtc acgccgaggg tgacgcttcc
    20941 gccgttggcc agcgacaccg gtgagccggc gatctggttg ccgccgcacg cgacggtgaa
    21001 ggtgaacgtg gtggggtcac cggcgatggc gccggcgatg gtcttcgaga tggtgagcag
    21061 tgcgccgacc cgatcggtga cggggttgga gatctcgttg atcgcgatgc ccgacgccgt
    21121 gaacgtggca ttggccacat tggtgatgac cgtgcccggg gcgacaccgg cgttgacggt
    21181 cacatcgaac cgcacgacgc cgaagcagag ttcgccaccg gcgcacgacg cgcccgtgag
    21241 gttgttgagc gtgccgaggt tcgcggtgac ggtgcccgaa ccgaacgatc cgcccgacgc
    21301 gagtgcggcc agcgctgcag ccgacgtctg gcccttgccg acgctgagcg aaccgggcac
    21361 gaacgtcgtg cccgccggga tcgaatccga gatggtcacg ttgttcagat cgacgtcgcc
    21421 ggcgacacgc accggcatcg tgtaggtgag gaggtcgccg gacttcacgt acggcgacgg
    21481 gctggtcgag ttgtcccggc cgacggcggt gccgttgacg gcctggtcct tctcgacgat
    21541 caacaggcac ttgaggggcg acgacagtgc gaactggccg gcgtagtagt tgtcgcgatc
    21601 ggtggtgaag gtgacgtcga tggagctggt gccgccgggc acctgcacga cctgggtctt
    21661 cgcgtcgatg ccgtgtccgt cgccgcggcc acccgagtag ccctgcgcga tcgacgcgcc
    21721 cgagtctgga tcggcatcga ggtcggcgtt gtggtcggcg ctcacctgct tgccggaatc
    21781 gaagaagttg tcggtgcccg acgggcccgc cgggttgacc gtggtcgagc ttcccgagag
    21841 gttcgacttg accttcatct gatcctcgtc gaggaactgg tcgccctccc acgccagaac
    21901 cccgaggcgg agggcaacgg gcccgggcga cgcagtgagg aagcccgaga gcgtcgtcgt
    21961 ggtgtccgca gcgccgttct gctgctggat caggccgtcg tatatggcga cgttccggta
    22021 gtcgttgcgg tggttcgctc cggcaccggc gtcgtcgttg gcgccggtca agcagttgag
    22081 cgtcgggaag cggtagacga cggtcagcga ccagccggcg tggcagttct cgccctgcat
    22141 cgtctgcacg ttggcgaccg acaccgtcgt cgcggtcgct gcggcgaccg tggagaaggc
    22201 ggcggtcacg tccgtgacgc cctcgtacac gagtccgtcg tcgctcgagc ccgatggcag
    22261 ggcctgtgcg agaccgccgc tcgggacggt gtagacggtg ttcgccgtga cggtcgtgta
    22321 ggcgccggcg cccaccttca tccggacggt gttggcgctg gacttgttcg cggtcgcgtt
    22381 ggagacctcc gcgtggttgt ccgagcagta gtactcggtc ccgttgctgc gggttgtccc
    22441 gaggttgcct ccccagtaga ggtaggcggc gtcgacgacg gcgcccgacg ggatcgtgaa
    22501 ggttgccgag ctcccgttga acaacgcccc gttcccgtcg gggtcgttgt tggccatgtt
    22561 gaggtcgtcg ttggtggtgc tgttggtcgt gcaactgctg cccgagcagt tgaggaccgt
    22621 gttgccgacc atgaggaagt cgccgttgac gttcttgttg tagcgggcgc tctgtcccgc
    22681 cccgaagtca gccgctgcct ctccgacggt gggcaccatc ggcaagccgc cgaggccgac
    22741 gaatgcgcac aacgcgatga cgcccaggcg tcgcgacgcc gtcgtgatcg atcggttcat
    22801 gtctggcttc ctcccaccga ccctgcccgg gtcggttggt tggagcgtgc agcaaccgat
    22861 ggcgcctgac aatacctttc atctcatttc agtgaattga gttgaggcgg tttcctcaac
    22921 aggagaggtt cgaacgtccg acggcgagcg atcggcggga ggtcagtgcg agagcgggat
    22981 ccggagcagg cacccgaact cccccgccac gtcggcggag acccgcgcgt gcacgcgacc
    23041 ctgtcgggtc cggaagtcgc cgtcaccgga ctccgggtag acgccgtcgt gccacacgcc
    23101 ggggaggacg cagataccga agctgccgtc gctgcgcgcg accactgccc gttcgaggtc
    23161 gggatcgtcc ccgaccggca ccaccgggac gaggaagggt cgcccgtcga ccgaccagaa
    23221 gagctggccg ccgtcggggt ggtggttgag atgccacaac aggatctgct cgcgcggccc
    23281 cgacgacggc gcgtcgagcg actcgggcgc gcaccccagc ccgatcacgt agtcgccgcc
    23341 gacggcagcg ttgcgggcgc ggagacgctg gccgtcccag acggtgagga acgtgccctc
    23401 ggtcgtgccg ccctcgatgc cggtgccggg atcgaggggg cgtggccccg acaacggcca
    23461 gggcacgatc ggcacctcga actcggccgg gtcgccttcg gcgatggcga gcagttcggc
    23521 gagtgacggg tcgcgtccgt cgacgacggg caggtcccgc cacggcaggc cggatgcgat
    23581 gtcgggatcg aggtactggg caggggtcac tccggcgtgg gtcacgtcgc cgaggctagg
    23641 tccgaccgct tccactccga ccgcttccac tcccactgct tccactccga gcggttcggc
    23701 ggtcagccgc cggggcgaac ccctgccggc ggtgtgccac cggtgaaccc agcgcctccg
    23761 gggaatcctc cgtcggggat gccgcctccg gggaatccgg tcggggtgga gcccgacgtg
    23821 ttcgaggcgc cggccgactc ggccgtcgcg gagcccggga gtgcggcgtc gaggtcggcc
    23881 aacacgaccc gctggcccga cgtcaacccg ctcgtgatct cggcccacgt cgtccccacg
    23941 acccctacct cgacacgggt ggacttggtg ccggtcgcgg tccggacgag gaccgtgtgt
    24001 tcggtgccgt tggtcgtgac agccgacgtc ggcacggcga gcgcttcccg cgtggcgccg
    24061 gtcacgatcg acaccgaacc ggtggccccg ctgtggaggt ccgtcggcgg ctcctcgagc
    24121 gccaacacca cccggtaggc cgtggaggtg gatgccgaat cgggcgtcac cgagatggag
    24181 acgacctcgg aggagagcgg ttcgtccgag ctgtcgagcg tcaccgtcgc ctgctgcccg
    24241 acctccacgg tcggaatctt ggcgaccggg atggtcgtcg tcacttcgaa gccaccgtcc
    24301 ccgacgacga tgatctgctg ggtcgtcgac gcggccgtga ccgactctcc ggtatcgaac
    24361 ccgaccgcga ccaccgtgcc cgagatgggc gcggtgagct ccgtctgcgc cacgttgtgg
    24421 gtggcgacga tcacgtcgag cgctgcagca tccacggcct tctgcagcgc gatcagctgt
    24481 tccgatgacg gggtgctgga cgtgccgctc gatgtggccg ctgctgccgg gagaggtgag
    24541 ctcgccggtg cggcggtcgt ggtggtgacc ggcgccccgc tcgctcctgc tgtgcttccg
    24601 ccgccggccg tgaccgcagc ggtcgatgcc gacaggaggc ggtcgagttc ctgggtcgcc
    24661 gacgacagcg ttgtctgggc tgccgcggtg gcggcctggg ccgagcggac cgtactcagc
    24721 gccgaacggc aggcggcgag cacgtcggtg gaaggaacgg tcggaggcac ggtcgtcgtc
    24781 gacgggctgg tcgtggtcgt ggtcgtggtc gtctcggtgc tgtcacagac cgcggccgcc
    24841 gactgatacg tcgcctcgga atcggtgagt gccgcgtcga ccgtccgctg cgcgtcgagg
    24901 acggcctgct gcgcatagcg cagcgcggcg gcgtcggtcg cggcggtacc cgaagcggag
    24961 gagcccgacg tcgtggtcga tgcgggcgaa ggggtcgcgg tggccgcagc ggccgtggtg
    25021 acggtggtcg tggcggtgcg ggcaccggtg accgacgacg cggtgggagc ggcgccggcg
    25081 ccaccggtgc tggtgggttc gccgtcgagc gccttggaca gggtgagctc ggcctgggcg
    25141 agcgctgcct gcctggtgtg gagtgtccgt tcgaggctcg ccgtgtcgac cgtcgccagt
    25201 gtctggcccg tcacgacctc atcgccgacc gccaccgcca ccgaggcgac ggtgccgctg
    25261 atcgggaagg cgaccgcggc ctggttcacg gcctcgacgg tcgcgacgcc gttgaggacg
    25321 gcctggacat cgtgtgtgcc ggcaacggcg gttcggtagt tcaacgcctc cgatccggac
    25381 gcgttgaccg cgacgaaccc cgatgcgacg agggcaaccg tggtgagacc gacgaggccc
    25441 caccgggctc ggcgggtccg ccagaacggg acgaccccgt cggggtcggg aaacagcgac
    25501 gacaaccgcg cgtcggcggt cgtggggcgg gtcgaagcgg gggcgtcggt catgtcctat
    25561 tcgttcctca acgcgtcgat gggggtgagt ttggcggcgc gggtggcggg gaagacgccg
    25621 aagccgacac cgatggccat ggccatggcg atggcggcga ggcaggcggg gatggagacg
    25681 agcacttggg agtcggtgaa gtgggggagt acttctgcgc cgatgatgcc gagtgccacg
    25741 cccaagatgc cgccggcgag gcccaagatg agggcttcga cgaggaattg gcggcggatg
    25801 agttgggggc ggccgccgag ggctttgcgg aggccgatct cgcgggttcg ttcggtgacg
    25861 gagacgagca tgatgttcat gacgccgatg ccgccgacga ggaggctgat gatggcgatg
    25921 ccggcgagca tgacggtcat ggtgtcgtcg acggtggagg ccgccgtcag gatcgactcc
    25981 tgggtggcga tcgagaagtc ggcgtcgctg gagtcggaga tgccgtgagt gttcaacagc
    26041 agccgatcgg tctcctggta ggccgctgac agtgtgtcgg cggaggtggc tttgacgtag
    26101 atgctggaga cggagttgcg gttggtgccg ccgacgaggc gttgggtgta ggtggagagc
    26161 gggacaatgg cgaggtcgtt gttggaggtg gactccgacg acgacaactc gtcgaggatg
    26221 ccgatgacgg tgagcttcgt accgttgtag gagacggttt ggccgacggc gttgccggtc
    26281 gggaacagtt cggtcgcggt gtccggcccg aggaccacga cggcggccgc gtcggcctcg
    26341 tcggtgtcgg cgaggaatcg tccggtcgac acgtcacgcg agcgcacctc cgaccagctc
    26401 tcggtcgtgc cggtgagcgt ggtggtccag ttggtggtgc cttggacgag tgagaccgag
    26461 gtggtggaga cgccggcgac ggcttgtacg tcgggggcga cgttgttgga ttcgagggcg
    26521 gcgacgtcgg tggtggtgag ggtggacgcg gtgccgaagc cgcctcgggt gccggtgctg
    26581 gcgtcggtgg tgctgccggg cgatacgacc aacaggttgg tgccgagttc gttgatctgg
    26641 tcgttgatct gggcttgggc gccttggccg aggccgacgg tgaggacgac ggcgctgatg
    26701 ccgatgagga tgccgagcat ggtgagcgcc gagcggagcc ggtgggtacg gaccgcctcg
    26761 aacgcgctgc ggagcgtgtc tcgccagctc atgtcggttc cgccggcatc gagtcgcccg
    26821 gcgcccagat tcggccgtcg cgcacttgga tgacgcgttc ggctcgttcg gcgacgtcgg
    26881 gttcgtgggt gatgaggacg atggtgcgtc cgtcggtgtg gagctcttcg aggagtccga
    26941 gtacgtcgtc ggtggaggtg gagtcgaggt tgccagtggg ttcgtcggcg aggatgagtg
    27001 cgggttcggt gacgagggct cgggcgacgg cgacgcgttg ttgttggccg ccggacagtt
    27061 cgccgggtct gtggttggcg cggttggcga gggagacctt gtcgagtgcg gccatggcgc
    27121 gttgtctgcg ttcggcgggt ggggtgccgg cgtagatgag ggggagttcg acgttgcgcc
    27181 aggcggggag gtaggcgagc aggttgaatt gttggaagac gaatccaatg aggaggttgc
    27241 gaacgtcggc gaggtggttt tcgtcgaggg tgccgacgtc gtggccggcg aggcggaact
    27301 ggccgccggt ggggacgtcg aggcagccga ggatgtgcat gagggtcgac ttgccggaac
    27361 cggatggccc ggtgatggcg acgaactcgt tctgctcgat ccggagggtc accccggcga
    27421 gggcggcgac ttcgacgtct ccggtgcggt agagcttggt cacgtcggtc agctcgatga
    27481 ccgggacggt cccaggtggg gtgaccgggc gagtcggcct gacggtcgtc gcagtcatcg
    27541 gctcgctccc gtcgctccgg cgccggtcgg agcgccggtc ggggcccccg cggcagcgtt
    27601 gccgacaccc cgacccggga agctgatcac gacctgctcg ccggccttca gccccgaggt
    27661 gatctcggtc atgccgctgg ccgtcagccc gatcgtgacc gatcgggtct cggtggcgcc
    27721 gtcggccgag ccgtcggtgc tgaccgtcac gctcgagccg gtgtcgtcgg tggagaccgc
    27781 tcgcgtcggg acctgcagca cgtcggttcg ctcggccgtc acgatgtcgg ccgtcaccga
    27841 cgacccgacg tagaactggt cggtggcacc ggtgaacgag acgaccacct ggtaggcggc
    27901 gacaccagag ctggcatcgg cgatccgact cacctccgtg accgatccgg tggcggtcgc
    27961 cccgctggcg cccgactgcg tcgtgccggt cccgcttccg ccgcccccga gctcagtcgg
    28021 gaatccaccg gtcgggaagc cgccggaggg gagtccgccg ctggggaacc cgccgaaccc
    28081 gccgcgtcgg ttggaggagc tcgtcgtcac cgcgacgtcg acggtctggc ccacctcgat
    28141 gctgtcgatg tcggtgctgt cgacggagac ctcgacctcg aaccgaccgc tgctgaccac
    28201 ttggatctgg gccgtcgagg aggacgagcc ggtcgactgg ttcaccgacg ggagcgcagc
    28261 agtcgagccg ccaccggtcg cagaggagcg acccgaaccc gtccctgatc ccgtgagggt
    28321 cgtgccgccc tgcccgccgc tcgacaactc ctcgccgacg gtgaggtcga cactcgcaac
    28381 ggtgccgtcg aaggtcgcca cgagcgatgc tccggcaagt gcctccgtcg cggtggcgag
    28441 cgtgtcgttg gccgtggcga tctgtgcgat gtcggcggcg atctgttcgt cggacgcatc
    28501 ggcgtcctgg tcgtcggcga gcttcgcctc ggcgtcggca accgccgact ccgccgacgt
    28561 gaccgcagcg gccagctccg acgagtcgat cgtcgccagt acctgcccgg ccgtgacggt
    28621 gtccccggcg gcgacgttca ccgcggtgac ggtgcccgac gacgagaagc tcaggtcgtc
    28681 ggtctgggcg gcggcgacgg ttccctcggc cgacaccgtt tcgttcatgg agccactggt
    28741 gacctccacc agctggttcg tcggcgcgac agcggtcgcc gtcgtggtgg tgtacgtgtc
    28801 ctcacggatg ccgaaccacc agacggcgac ggcgaccagc gcgatcgccg gcatgaccac
    28861 gaaggggtga aggaggcgcc gtctcacgat cggcccgcgg aggaggtgtg tcgtacctgc
    28921 atggaaacga gcatcgggcc cgttcctggg agttacctga gagcgatcgg ggagttcgtc
    28981 cggtgttctg ccgattcctc agagaaccct cagaaccgac ggccgtgcat cttgtcgaaa
    29041 tcggtggaac aactcagcac ttccccagga aggtctgagg aaccgggccg atcgttgtcg
    29101 ccatgagcta cgaatccatg ccgacccagc cgccgaccga tccgccgcaa ccgcccagcg
    29161 cgacgccggt ccaaccggcc gccgacgcga agcccagtgg gccgaccgcc aagacctgga
    29221 tcatcggtgg tgtgggagcg gtcgtcatcg ccgcggccgc catcgtcggc atcaacgcgg
    29281 cctcgtcgga caccacgacc gtcgacacgg cagcggcaac caacggcacg gctgccaacg
    29341 gatccaacgg cgctgcggcc gacggccagg gcggcttcgc tggtcgcggc ggcggcacgt
    29401 tcggcacgat cgagtcgatc gacggcgcca ccatcacggt ggccagccag gacgggacgt
    29461 ccaccaaggt cgtcacgacc gacgccacca ccgtcacctc cacgggcgaa gggaccgcgg
    29521 ccgacctgaa ggttggcgac aacgtccagg tcaccgggtc cggcacgtcg tccgacctcg
    29581 ctgccgagcg cgtggtcgac agcggcgacg ttgccgccac cggcgggttc ggtggacgtg
    29641 gccagggcca ggacggtcag aacggccagg gcttcccagg agggacgccg cccgagggca
    29701 tgagcattcc cgagggaatg accccgcccg aagggatgac gccgcctgcc gacggacaga
    29761 tgcccaccga cggacagatg ccgacgggag cggacggcca gcagctggtg ttcgcctccg
    29821 gcaccatcga gtcgatcgac ggctcgacga tggtggtgaa ggcgagtgac ggcactacct
    29881 ccacggtgac gctgtcggac acgaccacga tctccgtcgt gaccacgatc acggtctccg
    29941 atctcgctga gggcgacacg atcatggtcc gtggcgagac cgctgacggt acggtcaccg
    30001 ccacctcgat caccgagggc gccaccggct tcggcggcgg aggattcccc ggaggcggcg
    30061 ccccgggtcg gcctgatcag tcgggcggag ccactggctc gggttccgcg tcgaactgat
    30121 cggcaacgtc aggccggccc cgcgtttcga cgcggggccg gcctgattgc gttctcgggc
    30181 tcagccgttc gtcgcggtca cattggcgga gacggtggtg tgcctttcga acccgcccga
    30241 gtgcttggac tgcttgcaac aggcggagtc ctagctggct cgtgaactcc tgacgcgaag
    30301 gtcttcgacc ctcggcgctt gctccacgta ctcctccagg gcccagttca tgatcaggtt
    30361 cacagagacc tgacgctctc gggcgagggc cttgaggcgt tcgtccagct gcggcgagag
    30421 cctgaccgcc ttcgtcaccc tgtcatcgaa ctcgcggggt cgtgccatgg cggcagtcta
    30481 actacatcgc tgtagtttgt gaaggtattc gcgcgaaatc gcgcgcatta atgaccccag
    30541 aaattatcgg gtatccacca cttgtcagca ggtacccgtt acggtccgac ttggtcaaag
    30601 tggccaagct gagtcggcct gcggccacct ctcggcgcaa ggacccactg acgtgggaag
    30661 gagacaccaa tggcggatcg acaagtcact catgcacacc gcgacgatca ggggatcatc
    30721 ctggctgttt gctggaaagg tggcaccagc ttgatgtaca cggcgcggca gcaggtcgtc
    30781 gacgacatca gttcgggcgc tcaccggtac tacgtccatg aacaggcgcc cgcggtctgg
    30841 gtcgaggtca tcacggtgaa cggtgtccag tacatcaaga ccgaggcgga ttcgacctcg
    30901 cagaacaatc tcgacaacct tccgacgtgc actcagaaag ccgggtgaaa aacgcacgtt
    30961 tcttgggtac accagttcgt tgataaggtg cgtcggagaa gcgttgctca tccccgagtc
    31021 tgtgcagacc cgaggtggag ttgatcggct gggactacaa ccagaccttt gacgtactca
    31081 tgaccgccca cggcgaacct gtcaccgtgg cccggagagc atcccgtcgt ccaggccggc
    31141 cacgccgcgg cgggtagcac catcatgtcg aaataggcct gccaacgcag gagccccggc
    31201 tgtcaggaga gggcctacat gatcggtgtt cgaaaggcag accggcggag acggtgggct
    31261 tcgaacccac cgtcgggagc tgtgccccac gaggtggggc acagccgctc atcggggtca
    31321 cgtcggcgga gacgggtggt gtgctgtccc aggacatcgg aaacccatct tgtcgcgtca
    31381 tcggaaacgg ttcggttctc gtgtgagggg gtgtcgaaga accggttgat catcaccgct
    31441 gtcgttgtcg agggatggtc cccaagcgga cgtggcccgc gactacggcg tgtcgaaagg
    31501 gtgggtatcc caagctcgtc gcccgctatc gcgacaaagg cgacgccgta ccaccgggcc
    31561 cgccacgacc gcatcgacac caccggcaag atcacgctgc gccacaacag ccggctgcat
    31621 cacatcggcg tcggacgaaa acacgcccga acccacgtca tcgtcctcgt cgcacacctc
    31681 gatgtacgca tcgtcaacgc cgccaccggc gaactcctcc gccacctcac cctcaacccc
    31741 acccggggct accaacccac cggcaaaccc cgctcccaga aacgacaaac ccccgaacct
    31801 gagggttcga gggtttccta tgtcgtgaga gatcacactg cggagggggt gtggctacgt
    31861 tcgaactccg cgaggtggta tggtctggct gagctacttt cgcgttggcg tctagacgta
    31921 gcggaaggca gctgacggac tgatccgggt tgctccgatc gccggaagga agcccgagac
    31981 gaggccgata gtgagtccga ggaccggtgc cgccacgagg tagcgcgggt cgaggattgg
    32041 ctcacccgca ggtcggatgc tgagcaccag agctgcgcct gcccaacctc ccgtgaagcc
    32101 gccgagaagc cctgcaaggc aggtctcgga gagcacgagt acgaagatat gtatcggtcg
    32161 agcgcccatg acacggcgta ggccgatctc gggacgtcga gaggtcacag agctgtatgc
    32221 ggacagtcca cctgccagca cagctccggc aaggaccacc gcggctacga cgacgacgag
    32281 atcccgcacg tcgctgtcga cctgaccgcc gagactggag gtgtcaaaaa cagcggaccc
    32341 ttcgattgct tccggtcgat tcggcacgag cgcatcccca actgagcgtg cgacagagtt
    32401 ggcatagccg gcatccactt ggacggcgat cgttgtcgac ccgcctccca atgcaacctc
    32461 ggccgatctc gtgacgagta ccgaagtgag cagggccgga acttcgggcg acgacgtgat
    32521 cacaccgagg acgggacaag gtatgccgtc cacagacacg gtcgggccta agccaaccgg
    32581 accgagcgac agatctcgcg ctactcccga acccacgaac gcgccaccgc ccgacacgat
    32641 gctcctgtca gcatctgccg gttgaccggg gacgttgaga tccagggctg cctcgaggct
    32701 tccagcaaat cggactactg gtgcctggga agtgaaccca ccggagctga cgtcgcgctg
    32761 accgagctcg gtgagtacca cggcggccgt tgcgccattg atgtcctcga cgcgttgggc
    32821 ggcatcgtcc ggaagttcaa ctgaggtatc gactagctgg gcggtgacga acgtcgatcg
    32881 ctgcgctgcg aatctgtctg agacggatac gcgtgtggcc tcggcgaggc cgagcaccgc
    32941 aacggcactg ccgataccga ctgctgtgag cgcagcaact gttgcgatac gtcctgggcg
    33001 tgagaggacg gtcgatccga catcatcgag gtgggcgcgg atgcggagac ggctgcgaat
    33061 cgtgccagtc atcatgtgag gtgcgacggc gagggctcga cgcggccgtc gctgacgaca
    33121 agacgacgtt gcgcccgccg ggccacttcg tcggagtgcg tgacgcacac gatcgtcaag
    33181 cccgcagcgt gaaggtcgtc aaggatgtcg agtacgagcg ccgaggttgc agaatcaagg
    33241 ctgcctgttg gctcatcggc gaggagcagc ttcggctggt ggacgagcgc ccgtgcaatg
    33301 gcgacgcgtt ggcgttctcc acccgagagc tccccggcgt cggcctcgct gcggtggagc
    33361 agtccgacgc ggtcgagtgc cgctcgactc cgttcggttg ggtcaccgcg tccgccacgg
    33421 tagagcaaac cacgtgcgac gttctgggtt gcactcaacc tgtcatcgag caagaaggac
    33481 tgaaacacga agccgatcag ctctccccgc actgaggcgc gatcgccctc ggagcaagcg
    33541 atcatgtcga tgccctggag taggtacttg cccccagtgg gccgctcaag tccgccgagg
    33601 acggagagca gacttgactt accagcgccg gagcgcccgg tgacggcaac gtactcgccg
    33661 ttagccacgg tcacgtgcgc gttgacaact gcgttgacga tcggggggcc tggataggtc
    33721 agcgccaggt cgtgggcatc cacgatcaag gtcatcgagt tcctctggag ccgagtacga
    33781 ccttgtcgcc tcgacgcagc ttgccggtgt cgatgatctc cacccatcca tcgaccgtct
    33841 cgccgacgga cactgcgact cgtcgttgac gtttgccgtc cagcacgagg acgcgagcgg
    33901 agtcggattg ctcgtcctcg acgatggctg aactcggaac gacgagatcg gccgagcgag
    33961 gcggaacgag cgctacccgc agggtgactt cggatccatc aatgtcgagc gtgtccggcg
    34021 cgagtggttc tagttcgacg ttcaacgtgg aaggggccgt gccctcgggc agtgccgttg
    34081 cctctgtcga aacgatgcgg gtgaccttga gcgggatggg cggctgtccg tcggtgacag
    34141 cgtcgaggcc tgccgtgagg agcgccttct gggactgggt gatatcggcc cgcaccacct
    34201 ccggaccaga ctcgatggtc aggatggttt ggccgtcggt caaggattga ccgagagctg
    34261 cgtcaattga cacaacgcgt ccgggtagtg ccccgacgac ggcgagttcc ctggctggca
    34321 ctaccacacc ttcgatccga ctgaactccg tggtagcggc gcgagcagcg tcaagtgcac
    34381 tcttcgctgc tgcggtatct cccgtagtag ctcgaaggag actctcggca ttgctctctg
    34441 cgcgatcgag ttctacacgc ttcgcgagaa gctggcccag cgtgccctgg gtcgtcaacg
    34501 gggtgcagcc gatcgacccg taggtgtcga gaaccgcttg agcggtcgaa gacccgaata
    34561 cgcctggagg atcatcactt gggtgaccgc acgccgcgag agcggtttga agctgagaga
    34621 cgtcggtgcc gatgtcgccg gggcggagat cgcgatatgg ctggatcgct cccggtaggg
    34681 cgatgattgg acgatcggcg accgaaccca ggagcccaca tggcctcacg gtcgcgtcga
    34741 ctgctacgtc gaggcttgta agtaccggcc gattgccctc ggcggtgggc ggcgcaagca
    34801 cgtcggcacg atcgcggaag tgcttggatc ctcgaaggac gagatcgctc tggagtactc
    34861 gaggtccgac gacatacgtg accggtttgg ctgcgactcg tgacctctcc gtgatctccg
    34921 acggcgatga cgatgtcagt gcgacgacaa cgagggcggc gaagcccgct agcgggagga
    34981 taaccagccc ccaccgggcc acagttcctc cagctaagcg cttgcgcggg gcggacggca
    35041 cctacctgtc accactcgcc aagccgagcg atgcagcgat ttggtcgatc ttcttcgtgt
    35101 agtgcgtcgt agcttcccgc tgtgctcgca actgggactc gtgtccgacg gcttgacgaa
    35161 gctgctcgtc gtactcggtg gaatagacca acttgagata cccggtcgac tcctggcact
    35221 ttgcgtccgc agtcgcaacc tcgatctcct tcgccgcgac cggctcgtca gaagttggcg
    35281 aatctgttcc gtacaactct tccagtacct tcacagcgac atcaccgtag ttgtcggctc
    35341 cctcgacata ctggccgtag ccctgctcgc tcaggcatcg cctccatttc tcagtcgcgt
    35401 cgagaacctc gggatcggcc atggtggagg agtaggccgc cgatgcgtag tcgaagacga
    35461 gcgactgagg tggagccgga acctctttgc gcatcttggc gacgcattcg ttgaaggcgg
    35521 cctgctgctg atccgtgagc ttccgactgt tgagctccag ctgctccttc gtagcaccgc
    35581 cgtcctgccg gtagccaagc tcggacgcga ctttcaggtc aaagatgcga cgtccgaacc
    35641 ggttggtgga tttgggcgcc gccccgatga cgtctggcgc ggccggaatc gtccactcga
    35701 cacccttaga atccatgcag ttcttgacga gaacattttc cacgtagttc ggccatgtgc
    35761 tcgacgagac gctgacctca tctaacggca gcgcataggt cgcaaggtcg cggtgcgatc
    35821 caagttcctt ctcgacagac ccgagatcgt ccgacccgca gccggccgac aggaccgccg
    35881 tggcagaaac cacgacgatc gctgccgacc gagcaaggaa gggtcgcctc ggacgtaggc
    35941 cagggcgacc cagcatcagg cgacgcacca acgatgcgag tcgagtgagt cgttgaagcc
    36001 gacgggccct gacgcgttgc cgagatcgga gtcaccgctg ccgagtactt ggaggaagct
    36061 tgcgccgccg ccgctgacat ccttgtacat acgtacgttg caggagttgc cgaagttgac
    36121 caccgacgag acgctgtcgt gggggctcgt ggtcgtgttg tagtagtagt cgttgccgta
    36181 ggcattgtca tcgcccgacc aggcgccgct ctttgccccg tctccgaacg actggtagct
    36241 ggcgtccttc cagacgcaca cgtacccgcc gcccggacag ctcacggttg ctccagcgtc
    36301 caagctcacg gcccccacgc ccagcatcgc cactgcggca gcggcagcca accggcccaa
    36361 ccgcctgcca atcgccaatt ttgccattct aacccctcca ttattgttag tggatggcgc
    36421 cgactaggaa caccagatcg acgtcgggag actcttgccc ttgagtcggt ggtcgtgaca
    36481 agaatatttg gccgatcgag cgggagtgga cgagctcgtc gcgggtccta aagggtccgt
    36541 ggtggggact gctgcacgtt tcggtgacag gttgggtggg tggtcaggcc gcctgagcgg
    36601 cagggttggt catggtggtt tcgaactcga tcggggtcaa ccggccgagg gcttgttggc
    36661 gtcgccgtcg gtggtaggtg cgctcgatcc aggtcacgat cgcaatccgg agttcctcgc
    36721 gagtcgccca ccgttgacgg tccaagacgt tcttctgcaa gagcgcgaag aacgattcca
    36781 tcgcggcgtt gtcgccggct gaaccgacct ggcccatcga gccgaccatg cggtgacggg
    36841 cgagcgcccg gtggagtttc ctcgatcgaa attgcgatcc gcggtcggag tgcaggatgc
    36901 acccggcgac gtcggtgcgg cgggctgcag cgttgtcgag ggccgctaca gcgagccgtg
    36961 ccttcatccg actgtcgatc gagtagccga cgatccggtt cgagtacacg tctttgacgg
    37021 cgcagaggta gagcttgcct tcgttcgtcc agtgctcggt gatgtcagtg agccacagtc
    37081 ggttcggccg atccgcggtg aacacgcggc ggaccaggtc gtcgtgtgac ggggcaccga
    37141 ccttgccgtt cttcgagcgc ttcttcttgc cgaacgccga ccaccagccc atgtcgcggc
    37201 agatccgcca cactgtgcgt tcgcacacgt tgtggccggc gacgcggatc tcgtcgtaga
    37261 ggaacctgta tccgaactcg gggtcgtctc gatgcgcgtc gaagatcgcg ttggccagcc
    37321 aggcctcgtt cagttcggcg tcagtgacgg ggcaggcgag ccagcggtag tagtgctggc
    37381 gacagagctt gaggacccga cacgtcaccg tgacggggat tccgtcgtcg gcgagctcgc
    37441 tcacgagcgg gtagaacctt ttcccggcag gttcgcctgg gacagatacg ccgcgccgca
    37501 ctgccgctta cctcgctacc tggcggagac ggtggtgtgc tgtcccaggt cataggaaac
    37561 gggtctgtcg cgtcatggga aacgttggcg cccttgtgtg caggggtgtc caaagccagg
    37621 ttgatcatca ctgcggttgt tctcgagggt cgttcccagg ctgtggtggc ccgcgagtac
    37681 ggggtgtcgc cggggtgggt gtccaaactc gtcgcccgct accggctcga tggcgaggcg
    37741 g
//
LOCUS       DAOSOA010000005        33153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_1364_length_33153_cov_6.877002, whole genome shotgun sequence.
ACCESSION   DAOSOA010000005 DAOSOA010000000
VERSION     DAOSOA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 33153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33153
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1364_length_33153_cov_6.877002"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKY13_00875"
     CDS             complement(<1..233)
                     /locus_tag="PKY13_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78500.1"
                     /translation="MKRFWSYVSVVVIGLSVLGGFVLTDDAWPFAPLRMFSVGNNPDG
                     VVRAMRLQGDTAAGHLVLYADAFGLRRAELEEQT"
     gene            complement(233..2212)
                     /locus_tag="PKY13_00880"
     CDS             complement(233..2212)
                     /locus_tag="PKY13_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 1"
                     /protein_id="HOT78501.1"
                     /translation="MSLLEPIVLGSRTAPNRIVFGPHETNLAQGRAFSERHVAYYRRR
                     AAGGAGVIVVEEASVHVTDWPYERCPLASVAAPGWSAIAAACQAEGALVIAALGHSGG
                     QGSTAYSQMPLWAPSRVPEVNTREVPKSMEPGDVDELVAGFVAAATAAVEAGCDGVEI
                     NVGQFSLLRQFLSGLTNQRGDEWADRPALARRVLTDVRSAIGADRVLGLRLSCDELAP
                     WAGIVPEAGAELAADLAADADYVTVVRGSIFSSAATRPDGHVDPGFNLDLAGLVRTAV
                     RAAHGDRVPVIAQGSIVDPGQAEWAVAEGRCDAVEMTRAQIADADLVAKLAAGTPERV
                     RPCILCNQTCMVRDARNPIITCVVDPRTGHELTEPEPTGSTTAVTIVGGGVAGLEAAR
                     VAATSGAAVRVVEAATTTGGMLRTASAGSGRARLALFADWLDAECRRLGVEIETGHAV
                     EAAELGDSSAVIVATGSVASPLSVPATKAAVVLTAAEYLNAGSSSLPAGPILVWDPIG
                     GPIAVSIAEQLGWEGRSVTLATPDNIVGNELSRSGDLAPANARLQQAGVVLERRSILR
                     SVKKGTVVLEDRFTGAQRNLKAAVLIDCAHRLPDEALFRELHDASTGTVVRAGDAVAP
                     RTVLEAVREGRLAVAALDGSVRISDGVPMPRSTHH"
     gene            complement(2226..3410)
                     /gene="mftC"
                     /locus_tag="PKY13_00885"
     CDS             complement(2226..3410)
                     /gene="mftC"
                     /locus_tag="PKY13_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226361.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HOT78502.1"
                     /translation="MPARSSIPVIAVQPPAPRRSTLTDDMKRGLDAPICLTWELTYAC
                     NLACTHCLSSSGRRDPRELSTQECFAVIDELERLQVFYVNIGGGEPMIRKDFFEILDY
                     ANEHHVGVKFSTNGSYITAESARRLASMDYLDIQISIDGADADVNDAVRGTGSFAAAR
                     QAMEHLRAAEFGPFKISIVVTRHNVDQLDEFKAIADSYGAQLRVTRLRPSGRGADSWD
                     DLHPTNEQQRQLYFWLKDRPEVLTGDSFFHLSAFGEALPGLNLCGAGRVVCLIDPVGD
                     VYACPFVIHDEFLAGNVRDAGGFTSVWRESDLFLSLREPSSPGACGSCGSYDACQGGC
                     MASKFFVGLELDDPDPECVKGHAEPLLAALTPGNAPVSTVDHSRRSGSPVALTSKPVS
                     FQ"
     gene            complement(3397..3660)
                     /gene="mftB"
                     /locus_tag="PKY13_00890"
     CDS             complement(3397..3660)
                     /gene="mftB"
                     /locus_tag="PKY13_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03967.1"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HOT78503.1"
                     /translation="MVLDQAYELHPQVSLRPEPFGALAYHFGNRRLSFLRSPDIVNVV
                     TTLGDHASVADTLTANGVDARRWPSFQTALAALESSEMIRARS"
     gene            complement(3663..3833)
                     /gene="mftA"
                     /locus_tag="PKY13_00895"
     CDS             complement(3663..3833)
                     /gene="mftA"
                     /locus_tag="PKY13_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03969.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HOT78504.1"
                     /translation="MSPVTLTPDTAVAPVDVEALTDAVVTAEDAPVEDAELVETELLV
                     EEISIDGMCGVY"
     gene            complement(3935..4390)
                     /locus_tag="PKY13_00900"
     CDS             complement(3935..4390)
                     /locus_tag="PKY13_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78505.1"
                     /translation="MALYISAGRRRRTTIIAAVAAAIVGILLGFLLGRGTATTIDDRI
                     SASRDAGRKFAAALRVLPLEYEQAVTDDGESGGAAPIVERSLDGEQAALDAAPWLSDA
                     QIAALRSDLDVVRSAPANDLPPDEFAAAVDEAVTAVEATFGLAAGEASG"
     gene            complement(4411..6519)
                     /locus_tag="PKY13_00905"
     CDS             complement(4411..6519)
                     /locus_tag="PKY13_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOT78506.1"
                     /translation="MTASPAVRVGVDVGGTFTKAVAVDATGIVAQSVIATTHEHPNGV
                     AEGVVRAVADVAASLDGRPIALVTHSTTQAVNALLEGDVGTVGVIGMGRRPDLRKSRK
                     RTTLDDVELAPGKQLRTLCEFVDVSDGLDRGEIKRIVEGFVAAGASAACVAEAFATED
                     SSNEAVVAEIAAAAGLPVCTSTEMSGLYGLELRAVTAALNASILPIAVRTADVVAAGV
                     AAAGIDAPVMVMRGDGGATDLEGFRKGPARTLYSGPAASVAGVLRHSRVDDGIVIEVG
                     GTSTNVAAINAGRPALSYVRVASHSTALRAVDVRVIGVAGGSMLRARRSRFSGMGVYG
                     VGPRSAHIAGLRYACFLDTTEITGAAPELFAPKPGDPADYLGIRLVSGELVALTNTCA
                     ANAAGIIDAGDYAQGSTEAATAAFAVVGPMLGLDGAEVARRMLEASGNAVAELVAAVA
                     DEHDLVNPRIVAVGGGAGGLGRYTAKLLGLECSVPEHAEVISSVGDALSLIRTEREQT
                     VRNPTAAEIAELIAAAREDALAAGAAVESLDVRFEADPERSRVRAIATGAVALHSGAV
                     PGRQPIDEQEATRILTAAGAGGTARAEGAFWWAQTAGRNDLVRLVVLDRFGDVALDES
                     GALVVINAAASGGRSAELAGLAAAVEQHTRHLGPVALAPTVWVLTANQLLELGSGNHV
                     ETVTALVGDGTALALVTREH"
     gene            complement(6523..7890)
                     /locus_tag="PKY13_00910"
     CDS             complement(6523..7890)
                     /locus_tag="PKY13_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HOT78507.1"
                     /translation="MTAFAASDRARRFGVAALLAGALLTLLTLLGGGSAGASSTTAAS
                     PAAVTQGDATRETALDELHKVRDSIDETLALLKAGKRDEALAVARSGYLDHFELVEIP
                     LRAAAPELTLTAEEKFAEIRSSIKVEASTDEIRTQIIELRGLVDDAERQLTAKGLGAP
                     SLAFGQAFLIMFREGLEAVLLLSALLGYLEATKNTQYRKHIIGGVGLAVVATVATFFA
                     VDAVFAVLPFGREVLEAVVALVAVAMLFYVSFWLIARLEQRRWMEFLRAKVWTAVSVG
                     SATSLTLIGFTSVYREGFETALFFQALNSFGTGLGGYIAAGAVAGVVALAVVAFLIFK
                     LGRKLPIRVFMSVAVVLIMATSVAFLGNAIRALQEADKIGFTALSGWPRLQIFLAQAT
                     GYYPTAQGVIAQGALTAVYLLGALYMFVIRPRRSQARPAATDSGDHTVVELPDVTGSE
                     PALAN"
     gene            complement(7887..8324)
                     /locus_tag="PKY13_00915"
     CDS             complement(7887..8324)
                     /locus_tag="PKY13_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78508.1"
                     /translation="MSKPPHRALGRLAIVAMLVGPTFLGACTSDDSSNSDGGPTTTAP
                     EERVATDAEVADGLAKMQASVDELVAAGPDTARAAKADDELEPVWQEIEGTIKRNEPD
                     VYVDIEDSMSLLSSGVEGDEEKGALGAKDITAAIAEYLAKHPG"
     gene            8466..8894
                     /locus_tag="PKY13_00920"
     CDS             8466..8894
                     /locus_tag="PKY13_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78509.1"
                     /translation="MDARREAKLRKHLGTTGPIGDGCWIQRPGSPTGPSGLVLFGDTI
                     TFAASIAVSVALAARRSHRERQRPVKPLPDRPLALMISGDEVLVGRKGRVLRRYPTSV
                     LATVQRPRLSTEITLDDETFIAGGVEGDVLLKLRRSAPKP"
     gene            complement(8904..10271)
                     /locus_tag="PKY13_00925"
     CDS             complement(8904..10271)
                     /locus_tag="PKY13_00925"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT78510.1"
                     /translation="MAEVLDSFDHHNKTSTHVHDRAHVFHSWSAQALINPLPIARTEG
                     SWMWDFDDNKYLDFSSQLMNVNLGHQHPKLIAAIQEAAGRLCTIAPFHANADRSEAAR
                     LIIDVAPDNMDKVFFTNGGAEATENAIRMARLHTGRHKVFAAHRSYHGATHGSITMTG
                     DPRRWPSEPSIPGVVRYFGPYPYRSSFHSESPAQECERALAHLEELIMFEGAANVAAI
                     ILEPVVGTNGILVPPDGYLAGVRAICDRYGIVMIADEVMAGFGRCGEWFASQHWDVAP
                     DLICFAKGVNSGYVPLGGVIISEQIAATFDERVYPGGLTYSGHPLACAAAVASINIFQ
                     EEGTLAHATMLGTDVIGPELAAMAERHPSIGDTRGLGVFWAIELVRNRETREMLVPYN
                     ASGADAKPMADIVAACREQGVWVFTHFNRVHVVPPCNASPDDVRLGLAAIDKALELAD
                     AHVIG"
     gene            complement(10282..11760)
                     /locus_tag="PKY13_00930"
     CDS             complement(10282..11760)
                     /locus_tag="PKY13_00930"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOT78511.1"
                     /translation="MTHWIGGESTPGTSTRTSPVFNPATGEITRELPLANAADLDAAV
                     KVAKDAFGDWRRTSLGRRAEILFKARAIFDDRRAELADIVSSEHGKVPSDAMGEIARG
                     LENVEFACGIPNLLKGGFSEQVASGVDVFSIRQPLGVVAGITPFNFPAMVPMWMFANA
                     IACGNTFILKPSEKDPSASMFIAEVLAEAGLPAGVFNVVHGDKEAVDAILTHPDIAAV
                     SFVGSTPIARYIYETGTANGKRVQALGGAKNHMIVLPDADIDSAADAAISAAYGSAGE
                     RCMAISVVVAVGSAGDPIVDAIEARLDKVKIGPGTDPDNEMGPLITREHRDKVASYLD
                     VAADEGATVIRDGREQEFDGDGFFIGVSLVDNVTPWTRMHTEEIFGPVLSVIRVDTYE
                     EAVQLINDHEFGNGCAIYTRDGGAARQFTFDVECGMVGINVPIPVPVSYYSFGGWKSS
                     LFGDSHMYGPDGIHFYTRTKVVTERWPDPSTSQIDLGFPTNR"
     gene            11907..12449
                     /locus_tag="PKY13_00935"
     CDS             11907..12449
                     /locus_tag="PKY13_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78512.1"
                     /translation="MRRTLAIALVITAAGTVGCGDGTPVRDITPDPTTATAPTLPTTI
                     PVIAETTSLGGDADPAALAPATLPDVSDPILASYQPALDGLDSVLGTEGTTAALVRIQ
                     IATPTQAWSPEMLAALFASFCNDWESDDSAGEGVGPLTEQGRSDWAAAIVAPLHLSPF
                     DARSAVDWALADVAFTPPCQ"
     gene            complement(12463..13488)
                     /locus_tag="PKY13_00940"
     CDS             complement(12463..13488)
                     /locus_tag="PKY13_00940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT78513.1"
                     /translation="MADPDFSSLSPRPLGSLGLSAAAQGLGCMGMSEFYGTTDDATSI
                     ATLDRARDIGVTFWDTADVYGPHANELLLGGYFRTNPGSRDEITLATKFGIVRNVDDP
                     TYRGLNGRPEYVRSSCDASLTRLGVDHIDLYYLHRPDPTIEIEETFGAMAELVEAGKV
                     RYLGISEAAPNTIRRAHATAPISAIQSEWSLWSREIEDEVVPLARELGIGLVPYSPLG
                     RGFLTGQITSEADFDDDDFRKGMPRFTGENFAKNLDLVAAVKEMADERDCTPGQLALA
                     WLHAQGDDVHPIPGTKRPAYLGQNAAAVAITLSPQDLARLDEISPKERVAGARYADMR
                     FVNIESR"
     gene            complement(13499..14437)
                     /locus_tag="PKY13_00945"
     CDS             complement(13499..14437)
                     /locus_tag="PKY13_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOT78514.1"
                     /translation="MIHFRIVCPPDRTDELLSSLLVDEAVINIVSMVGAARRPDGDLV
                     QFDAAPESGNRVVDLLRSLAIDDLGSISIERIDTSLSDAARRAELHAPGHSAEAVIWE
                     DVEARSRDDSGTSPSFLALMMISALIAAIGILTDSPILIVGAMVVGPEYGPIASIAVG
                     IYRRRPARVRRGLRSLAWGLAAALAAVTVLALIVDGFGWTTDAYLSGERPLTSFISAP
                     DGYAVVVAVLAGVAGALSLTQLRSGTLVGVLVSVTTIPAAANIPVALVHDRPGEAGGA
                     ALQLALNIGLLCLVGAGTLALERALLDRRHRSLASE"
     gene            complement(14434..14805)
                     /locus_tag="PKY13_00950"
     CDS             complement(14434..14805)
                     /locus_tag="PKY13_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT78515.1"
                     /translation="MTLKLSEAYPDHVFASDCPSRIVLDHVTSKWGVLVLVGLAEESP
                     LRWGELRRRVQGVSEKMLAQTLRTLEADGFVSRHAFAVVPPHVEYALTPLGEELAERL
                     VDLMSWIVEHADGIVGAAASA"
     gene            14917..15780
                     /locus_tag="PKY13_00955"
     CDS             14917..15780
                     /locus_tag="PKY13_00955"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT78516.1"
                     /translation="MTTILVTGTSGHLGRLVVESLLENGTSPGDIVATARDISAIADL
                     ADRGVTTRTADFADPASLDAAFEGVDRILLVSGNEIGQRVAQHSNVIDAAKRADVELL
                     AYTSIPKADTSSLGLAVEHRATEQVLATSGVPYALLRNGWYFENYTAQIPVQLEHGVV
                     LGAAGDGKISGASRADYAAAAAAVLVADDQAGKIYELGGDAAFTLADYAAALAAASGR
                     EVVYQDLTSSDYAAALASNGVPQEFADLLANSDRGVAAGDLFVDSGDLTNLIGRPTTT
                     LADAIAAALPA"
     gene            complement(15857..16525)
                     /locus_tag="PKY13_00960"
     CDS             complement(15857..16525)
                     /locus_tag="PKY13_00960"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT78517.1"
                     /translation="MRYQHPHQLPHQHLDDRFLGDDDRRGPHFGGPRFGRGPRGDGPP
                     FGPPFGPGFGPPFAGRRGGRGRVGRGQIRTALLALLDEAPMHGYEMIRQIDERSGARW
                     KPSPGAVYPTLQQMADEGLVTVVEEGGKRVFSLTDDGREVVADLSAEDREPWTADADG
                     NPMWELKPLMKGLAMAAAAAMAGTPDQVERAKAILNEARRSLYAILAEDPAGATDDAN
                     VDES"
     gene            16620..18023
                     /locus_tag="PKY13_00965"
     CDS             16620..18023
                     /locus_tag="PKY13_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT78518.1"
                     /translation="MSDHSQPQPWGGDAVSLVDAFRSGERSPLEELDATLAAIEASDL
                     NAFSFLDTERAREAAADADVSKPFGGVPLAVKELDAVAGWPFTEASFPLRDQIAGFTL
                     THIERLRDAGGAVPFGLTTASEFGGVNLTRTVLNGATHNPWQHGRTPGGSSGGSAAAV
                     AGGLVTLATGGDGGGSIRIPAGFCGLVGLKTTYGRIPRGPHFEVGALTVTIGGLTRSV
                     RDTARWLDVANGADRHDPHSLPRVDGWEAGLGGHLHHMKGLRVAIIDRFAGAVVSPTT
                     AAVVREAGEQLARDLELTVVDVDVTIPNVGTAWSLANMAGTFAALGDAWPACADDLTP
                     EMRFACEWAAGAGYGMDARIKLEHRRTALFEAMATLFEQTDLVITSANPDVAFGAEGP
                     LPDTFGGVQDTPANNGRFTIPANLYGNPGISVPVGQVDGLPVGMQIMAPHHHESWLLD
                     AAWVVERERPWPLTAPA"
     gene            18102..18920
                     /locus_tag="PKY13_00970"
     CDS             18102..18920
                     /locus_tag="PKY13_00970"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT78519.1"
                     /translation="MKYVPMKRVLVAAAVGGSFLLGSAFGIDGVSAGPNDPSVGRASV
                     RVVDMTQPISPDMPIWPGDPAVEFDDLAQIPTDGYFLRGFGMGEHSGTHLNAANSFVE
                     GGRSIDQYTGRELVAPAVVIDVRGEVSRNADYALTIDKVRQWERRNGQIRPGSVVLLR
                     TGWEDRWSDPAAFFNEDADGALHFPGFAGDTTRWLYDQRRIGGVGIDTHGVDPGLDES
                     FATNVLTAERSKIALECLNNIDSLPATGATLVIGPTKLVGGSGAPASVTALVPR"
     gene            complement(18969..19973)
                     /locus_tag="PKY13_00975"
     CDS             complement(18969..19973)
                     /locus_tag="PKY13_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT78520.1"
                     /translation="MDLHRSVGLEPSTLRRADIAAATGADPERTIRWWRAMGFPEVAD
                     DTVAFNELDLDLVMRVAELEDAGLVDDDDIARLARLLGASFQRIAEAQLAMVDEMLAK
                     APDADPELLHRERLRKLLDEDDPALMKLLERSVTYVWKRHMLAALGRRLDPRLDPALD
                     GHDDTDVLAVGFADLSGFTKFSKGLTPAELTEVIDAFEAAAFEVVAPTHGRVVKLIGD
                     EVMFVAPTLSEAVHIGLDLRDRLAEFDGMPTIHCGIAVGPTVGVGGDVFGSTVNLASR
                     LTSIARRGTVVIPRECSTELTAELDLVVKPSRRNYSLKGIGDTRISVVGRAVPAPASS
                     "
     gene            20074..21189
                     /locus_tag="PKY13_00980"
     CDS             20074..21189
                     /locus_tag="PKY13_00980"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HOT78521.1"
                     /translation="MESRAAVLWGIGEPWKVEDITIDEPGPGEVLLKTKAAGMCHSDE
                     HVVTGDMLMPHYPCIGGHEGAGEVLAVGPGVTSVAPGDHVSMSFIPSCGRCKWCVSGR
                     QNLCDLGAQLFDIGMITDGRIAHKVGDTTLARMCQVGSFAEHLLVAESAVVKVEPDIP
                     WHAVALVSCGVATGFGSSVHRAEVKPGDNVAVIGCGGIGMNAIQGAKIAGAKRVIAID
                     PVEFKREKAMEFGATHTYSSIEEAQAEISALTWGEMCDKTILTVGVMTGDLIEPALGL
                     TSKGGTCVVTAVANMMENDAKLNSFMFAMMNKELKGCLFGSGNPRADIPMLLSLYREG
                     QLKLDELVTKTYTLDQINEGYQDMRDGKNIRGVIVFD"
     gene            21200..22174
                     /locus_tag="PKY13_00985"
     CDS             21200..22174
                     /locus_tag="PKY13_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT78522.1"
                     /translation="MVSYSRELVDRLDGSVVGRRSEIEQVVAGLAAGRHLLLEGPPGT
                     GKSTLLRRVAGELDRGFCFVEGNAELTPARLIGTFDPAAVLEAGYSPDVFLDGPLVTA
                     LKDGALLYVEEINRIPEETLNVLISVMSEGSLQVPRLGAIDAAEGFRLIAAMNPFDAV
                     GTARISGAVYDRVCRLGMGYQRADEEMLIVGNQARTAAATVGPGFIVQATEMVRATRE
                     HPDLRTGSSVRGAIDLTMVAAELRVLRDLPATDPGVTRDAAQLALSGRVRVRDGCGRT
                     AAEIVDELWAIFFAPEADDDPETGEPDGDDSTGKGQRHHLRAVAGPTR"
     gene            22279..23106
                     /locus_tag="PKY13_00990"
     CDS             22279..23106
                     /locus_tag="PKY13_00990"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT78523.1"
                     /translation="MGELDERAFERAMDEDLDEALTLLADLTGATDERLRELARTLAG
                     RVLVNIARTGVPRDRGVGRIRPRRASSADGDVDLDASLEAIVAARAERRPPAVDDLTV
                     QAWARPDTALCLLVDRSGSMKGDRLAAAAIAAAAVLYRHGRDCSVVAFASTAIVVKAQ
                     DERRDADEVVGDLLRLRGHGVTDLGLALRTARRQLERSSAGRRVTLLLSDARSTTGDD
                     PTADAAALVALGDLAVIAPEGDTADAEALATSVGGRWVSLSGPSTIPGAIAEALLQS"
     gene            23200..24849
                     /locus_tag="PKY13_00995"
     CDS             23200..24849
                     /locus_tag="PKY13_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT78524.1"
                     /translation="MAPKTPLVAPGDATAASSGTEPEDTYGIDPAIYHRRWLILSVLC
                     ISLVTIVVAVSSLNVAIPTIIRELGANSTESLWIIEAYALVFAGVLLPAGALGDRFGR
                     KWALLGGLVIFGGMALVASFAGSPAQLIAARAVMGIGAALIMPATLSIITNVFPPHER
                     QRAIATWAGLAGAGGAIGPLMSGIVLNAGWWWGSVFLVNIPLVVVLIGLVWFLVPNSK
                     NPNGEALDPPGAVFSVIALGGLVFAIIEGPEWGWLSGGVLGTFALALLGGAAFIAWEL
                     RAAHPMLDPRLFKLRGFGMGSLAITLAFFCMFGTFFLISQYLQFVHGYSALGSAVRTL
                     PSAVVMIIVSPRSPKIVARFGVRNCVRGGFGLITLGFVGMSTLGVTSAYWHLAIFLCV
                     MSTGMATLMAPASGMIVSSLPLSKSGVGSAVNDVTREVGGALGIAIMGSVLASGFSSK
                     MADRIKDLPIPAGPRELIEDSIGKAFGVSDEAVRSGLIPRSAGELIHDSAREAFVNGS
                     RIAFFVAAAVAAVGALVAGNLIPDETPEHGSVVDPVPASAG"
     gene            24857..25438
                     /locus_tag="PKY13_01000"
     CDS             24857..25438
                     /locus_tag="PKY13_01000"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT78525.1"
                     /translation="MTAAERSGLDPRVARSRAAVIEAALDLLGDAGYGGTSIDAISRR
                     SGVARTTIYRHWRSLAEIVHEAAMTTVGVKAVPDTGDARADLRAHLGELAAKLNSGAW
                     GRMLPVLVDAAFRDEEILELQRRSTAERKAAAMAIAARGVELGQIRDDVDLVLCGEML
                     VGPIFTRHLVTHLPITEAFLDDLIEATFRMIGA"
     gene            25499..25951
                     /locus_tag="PKY13_01005"
     CDS             25499..25951
                     /locus_tag="PKY13_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78526.1"
                     /translation="MEIIRNDDGTLTVPVEPDRQAVEAAAESGETLTATTMTLHPGEG
                     GYDEALSEWDLQQHDGQAAVSTASGREAAMHVIHEVAADPDIDIADAVEELDDPDASA
                     EALRHVLVGGVHSVEDFAAEVADHEGGDVLPPHEVTKIIGEVLAEIDS"
     gene            26178..26909
                     /locus_tag="PKY13_01010"
     CDS             26178..26909
                     /locus_tag="PKY13_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT78527.1"
                     /translation="MKDELVAALHGPAEQLAALLSERRDELPEVWALKTLSEDPVGDL
                     HKDNFAHTVKVVEQCGDTGDVTLLAALFHDVGKPLTRRVERGEVTFHGHEIVGAKLIR
                     KRFAVLELDPEPVIRTIMIAARAQSYLDSWTDSSVRRIRLDAGDDWDHAIRLAHSDVT
                     SRHAYVHRRVHQTVDALTTRSDDLLVEDARKAERPVLNGDDLAELGVPRGPEMGRLLK
                     WLLEENRAGRLTERPEAEAAIRSHF"
     gene            complement(26928..28898)
                     /locus_tag="PKY13_01015"
     CDS             complement(26928..28898)
                     /locus_tag="PKY13_01015"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HOT78528.1"
                     /translation="MSEPLWVPDPAGAASSAMARFRDAHGFADYPELHRWSVAEPDAF
                     WAALWDWTGMIGDRGERVLDLGATMPEARFFPDARLNVAEVILDGRGADEESWMYVGV
                     DERSGEGSAVTRAEVRRTVVDLACAMRAAGVQPGDRVAAWMPNIPTTMMVMLAAASIG
                     AVFSSTSPDFGVDGVVDRFGQIEPVLLFAADGYAYGGKSLDCVDRLATIRAALPSVRQ
                     VIVVGHRQGYGADPVGEWRAIPDAIGWGRFLTTAPAEAVASFEFERFPFDHPWYVLFS
                     SGTTGVPKCIVHRTGGVLLQHLKEHQLHCDIGPGDHVAYYTTCGWMMWNWLASVPASG
                     ATAVLYDGSPFHPGAAAMTDLIEAQDLTFFGTSAKYLDSLRKDGFVPREHAAMSSLRT
                     IASTGSPLSPETNTWAVESFPAGVHLASISGGTDLCACFVAGDPTSPAWAGEIQRPGL
                     GMAVDIFDEQGRPAGVDERGELVCTAPFPSMPLGFWADDDGSRYRAAYFDKNPGVWSH
                     GDFASWTEHGGMIVHGRSDTTLNPGGVRIGTAEIYRRVEQVDSVVESLVFGQDWEDDS
                     RIVLLVRLAPGVALDDELSAEIKHTIRVGCTPRHVPAVVLAVDDLPRTRSGKLAELAV
                     TDAVNGREVRNTSALANPEAIDAIATRPELRS"
     gene            complement(28989..30242)
                     /locus_tag="PKY13_01020"
     CDS             complement(28989..30242)
                     /locus_tag="PKY13_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT78529.1"
                     /translation="MEPVLRSAVGGALAIVNARKPRIRSRVLTIPSFFSGWITTEAAG
                     LLLLRRAVHAVRAWRLGRYRTTQGKVALALDAVTMAGLVGLIQQGRRTDEEFEAVLAP
                     YLDAETLAARPSSVRNGAIIPLFTGASTTKRMRNVGYVEDARWRNKLDVYQPKRVEAA
                     AHEQVPEGEAPKGSVPRPAIVEIHGGGWILGDKNQQGVPLLHHMVKNGWVGFNINYRL
                     APKAKFPEQLIDCKRAIAWIRRNAVDLGVDPDFICVTGGSAGGYLTSMMALTANDPEF
                     QPGFEDDDTTVQAAVPFYGVYDLEDSNHAMANGFDDFLRYVVVGTKMADDLDRYRRFS
                     PLHRVHADAPPMMIVHGTPDTLVPVEQARPFAEAMAKTSEHAVVYAELAGANHAFDIF
                     PSPRSVRTTEYVERFLSGVHLGLIK"
     gene            30375..>31873
                     /locus_tag="PKY13_01025"
     CDS             30375..>31873
                     /locus_tag="PKY13_01025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOT78530.1"
                     /translation="MQRLSGLDATFLYLETPSAHMHVAMTGIYDTSTMPGGYSFEAFR
                     DHIVGRFHRIPAFTRRLVEVPFQLHHPVWIEDPHFDVDYHVRRIAVPAPGGRRELAEV
                     AAQIASIPLDRTRPLWEIWVIEGVKQNRIGFVAKIHHSAIDGASGAEIMTELYDLEPN
                     PPARPPAEPAEPEHVPSDLELVTYALASKLRRSRDMVPLVGRTVQSVSNVVSNRRNPD
                     GAVGAVPLTAPRTPWNGAISPKRSVAFARIPLDELKAVRRALDVKVNDVVLAVCAGAL
                     RSYLIAHDELPDGPLLAVCPVSVRSDEEVGEGGNKVSAMFTSLATDIDDPLERLRTIS
                     DSTVGAKAEHNAVGARMLTDWGEYAAPRTFAMASRLYSSMSLADHHRPIHNIVISNVP
                     GPPFPLYLAGAELVAAYPMGPIMEGAGLNVTVLSYRDAVDFGFMADPALVPDVWEMAD
                     HVQEAFRELKDAAGIGVESAEPAPAAPEPAPEPAASRRRPSVVQKSARSV"
     assembly_gap    31874..31973
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            32055..>33153
                     /locus_tag="PKY13_01030"
     CDS             32055..>33153
                     /locus_tag="PKY13_01030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT78531.1"
                     /translation="MSSTIAPPARPVRPASGSGTPGPDAATSPDRSGALRLPDLAVAL
                     FAGVWLTSSWERIGAPLGLSHDGQNAGVFGSAARKLVDDPFGSHLGSRLANGHGVYSN
                     HPPLLNWLLGIGHAVTGDNPAGLRVPMVIAALVLIAATYRLLRLLVSSPWAAAIGTIA
                     GLGLPMFFVYGSMPDTPMLGLPFGVVLLGQVVAAETTPPKRSWLVVATAAGCALAGWE
                     AVFFAVIIATVALVRGRRGDRTSTTTGRAVFTGLAIGGAVTIAWIAWADGGLTRLADQ
                     FLFRSGAADVPFTVGEAVVAQIQYLADVYGIILVLAMFAALPALRIPAVRRHLPTVVG
                     VCLVYHVGFWQGATYHDYWSYWWQLPMAMCFA"
BASE COUNT         5114 a        11524 c        11472 g         4943 t
ORIGIN      
        1 gtctgctcct cgagctcggc gcggcgcaac ccgaacgcgt cggcgtacag caccaaatga
       61 ccggcggccg tgtcgccctg gaggcgcatg gcccgcacga ccccgtcggg gttgttgccg
      121 accgagaaca tccgaagcgg cgcgaacggc cacgcatcgt cggtgagcac gaacccgccc
      181 agcaccgaca agccgatgac gaccaccgac acgtaggacc agaaccgctt catcagtgat
      241 gcgtcgaccg gggcatcggc actccgtcgc tgatgcgcac cgacccgtcg agcgccgcga
      301 ctgcgagacg cccctcgcga accgcttcga gcacggtcct cggcgcgacg gcatcgccgg
      361 cacggaccac ggtgcccgtc gacgcgtcgt ggagttcgcg gaacaacgct tcgtcgggca
      421 ggcggtgggc acagtcgatc agcaccgctg ccttcaggtt tcgttgagca ccggtgaagc
      481 gatcctcgag gacgaccgtg cccttcttca ccgagcgcag gatgctccga cgttcgagga
      541 cgacccccgc ctgttgcagg cgggcgttgg ccggagcgag atcgccggag cggctgagct
      601 cgttgcccac gatgttgtcg ggcgtcgcca gggtgaccga tcggccctcc catccgagct
      661 gttcggcgat cgacaccgcg atcggtccgc cgatcggatc ccagacgagg atcgggcctg
      721 cgggcagcga ggagctcccc gcgttcaggt attcggcggc ggtcaacacg accgcggcct
      781 tggtcgccgg aaccgacagc ggcgacgcga ccgagccggt ggcgacgatc accgccgacg
      841 agtcgccgag ctcggcggcc tcgaccgcgt gacccgtctc gatctcgacg ccgagccgac
      901 gacactccgc gtcgagccag tcggcgaaga gcgccaggcg ggcgcgaccg ctgcccgccg
      961 acgcagtgcg gagcatgccg cccgtcgtcg tggcggcctc gacgacgcgg accgctgcac
     1021 ccgaggtggc cgcgacccgg gcggcctcga gaccggcgac gccgccaccg acgatcgtca
     1081 cggccgtcgt cgatcccgtc ggctcgggct cggtgagctc gtggcccgtt cgcggatcga
     1141 cgacacacgt gatgatcggg ttccgggcat cgcggaccat gcaggtctgg ttgcagagga
     1201 tgcagggacg aacccgctcg ggcgtgccgg cggcgagctt cgcgacgaga tcggcgtcgg
     1261 cgatctgggc acgggtcatc tcgacggcgt cgcaacggcc ctcggccact gcccactccg
     1321 cctgtcccgg atcgacgatc gagccctggg cgatgaccgg cacccggtcg ccgtgggcgg
     1381 ctctcaccgc cgtgcgcacg aggccggcca ggtcgaggtt gaatcccggg tcgacgtggc
     1441 cgtcgggtcg ggtcgccgcc gacgagaaga tcgacccgcg cacgacggtc acgtagtcgg
     1501 cgtcggcggc gagatctgct gcgagttcag cgcccgcctc gggcacgatg ccggcccagg
     1561 gagcgagctc gtcgcagctg agtcgcagcc ccagcacacg gtcggcgccg atcgccgagc
     1621 gcacgtcggt gagcacacgg cgagccaacg ccggacggtc ggcccattcg tcgccccgct
     1681 ggttggtgag gcccgacagg aactggcgga gcagcgagaa ctgcccgacg ttgatctcga
     1741 cgccgtcgca cccggcctcc actgccgccg tcgccgccgc cacgaacccg gcaaccagct
     1801 cgtcgacatc gccgggttcc atcgacttcg gcacttcgcg ggtgttgacc tcgggcacac
     1861 gcgacggcgc ccacaggggc atctgcgagt acgcggtgga cccctggccc ccgctgtgac
     1921 ccagcgccgc gatcacgagc gccccctcgg cctggcacgc cgcggcgatc gccgaccaac
     1981 ccggggcagc aaccgacgca agggggcaac gctcgtaggg ccaatcggtg acgtggaccg
     2041 acgcctcttc gacgacgatc acgccggcac caccggcagc acgccgccgg tagtaggcca
     2101 cgtgtcgctc gctgaacgcc cggccctgcg cgaggttggt ctcgtgcggt ccgaagacga
     2161 tgcggttcgg cgcagtgcgc gaccccagga cgatcggctc tagaaggctc acgcgacggc
     2221 tctggtcact ggaacgagac gggcttgctg gtcagtgcga cgggcgagcc ggaccggcgg
     2281 gagtggtcga ccgtcgacac cggcgcgttg cccggggtga gcgcggcgag cagcggctcg
     2341 gcatgaccct tgacgcactc gggatcggga tcgtcgagct cgaggccgac gaagaacttc
     2401 gatgccatgc acccgccctg gcacgcgtcg taactgccgc acgagccaca ggcgcccggc
     2461 gacgacggct cccgaagcga caggaagaga tccgattcac gccagaccga cgtgaacccg
     2521 ccggcgtcgc ggacgtttcc ggcgaggaac tcgtcgtgga tcacgaacgg gcacgcgtac
     2581 acgtcaccga ccggatcgat gagacagacg actcggccgg cgccgcagag attgagcccg
     2641 ggaagggcct cgccgaacgc cgacaggtgg aagaacgagt cgccggtgag cacctcggga
     2701 cggtccttca gccagaagta cagctgccgc tgctgctcgt tggtcgggtg caggtcgtcc
     2761 cacgaatcgg cgccgcggcc cgatgggcgg agacgggtca cccggagctg ggcaccgtag
     2821 gaatcggcga tggccttgaa ctcgtcgagc tggtcgacgt tgtgccgggt caccacgatg
     2881 ctgatcttga acgggccgaa ctccgcggcc cgaaggtgct ccatcgcctg gcgggccgca
     2941 gcgaacgaac ccgtgccacg gaccgcgtcg ttgacgtcgg catcggcacc gtcgatcgag
     3001 atctggatgt cgaggtagtc catcgacgca agacgacggg cgctctcggc ggtgatgtag
     3061 ctgccgttcg tcgagaactt cacaccgacg tggtgctcgt tggcgtagtc gaggatctcg
     3121 aagaagtcct tgcggatcat cggctcgccg ccgccgatgt tcacgtagaa cacctgcagc
     3181 cgttccagct cgtcgatgac ggcgaagcac tcctgggtgc tgagctcgcg agggtcgcgc
     3241 cgacccgacg agctgaggca gtgggtgcac gccaggttgc aggcgtaggt gagttcccac
     3301 gtgagacaga tgggggcgtc gagcccgcgc ttcatgtcgt cggtgagcgt cgaacgacgc
     3361 ggcgccggcg gctgcacggc gatcacggga atcgagctac gagcgggcac ggatcatctc
     3421 cgaggattcg agggcggcga gcgcggtctg gaaggacggc caacgtcggg cgtcgacgcc
     3481 gttggcggtg agggtgtctg ccaccgacgc gtggtcgccc aaggtggtga ccacgttcac
     3541 gatgtcgggc gagcgcagga agctgagccg ccggttgccg aagtggtagg ccagggctcc
     3601 gaagggctcg ggtcgcaggg agacctgcgg gtgcagctcg taggcctggt cgaggaccac
     3661 tctcagtaga cgccgcacat gccgtcgatc gagatctcct cgaccagcag ttcggtttcg
     3721 acgagctcgg cgtcctcgac gggggcatct tcggcggtca cgacggcgtc ggtgagggcc
     3781 tcgacgtcga cgggggcgac agcggtatca ggggtcaagg tcacggggct catggatcgt
     3841 cctctcgagg gggcgggaaa tcgcccccac ggtactgggt cggggatggg gccaaccacc
     3901 acggcgaccg gcactgattc tggtgggcgc tccgtcaacc ggaggcctcg ccggccgcca
     3961 ggccgaacgt cgcctcgacc gccgtcacgg cctcgtcgac ggcagccgcg aactcgtcgg
     4021 gtggcaggtc gttcgccggt gccgaccgga ccacgtcgag gtcggagcga agggcggcga
     4081 tctgggcgtc gctcagccac ggcgccgcgt cgagcgccgc ctgctcgcca tcgagagaac
     4141 gttccacgat cggtgcggca cccccggatt cgccgtcgtc ggtgacggcc tgctcgtact
     4201 cgagcggcaa cacccgcagc gcggccgcga acttccggcc cgcatcgcga gacgccgaga
     4261 tccggtcgtc gatggtggtc gccgtgcccc gaccgagaag gaacccgagg aggatgccga
     4321 cgatcgctgc ggcaaccgcg gcgatgatcg tggtccggcg gcgacggccg gcgctgatgt
     4381 agagggccat gcctcggtgc tcctgtcttc tcagtgttcg cgcgtgacga gtgcgagtgc
     4441 ggtgccgtcg ccgaccagcg ccgtgacggt ctcgacgtga ttgccggagc cgagttccag
     4501 cagttggttg gccgtgagca cccagaccgt cggcgcaagc gcgacgggac cgaggtgccg
     4561 ggtgtgctgc tcgaccgcgg cggcgagccc ggcgagttcg gcactgcgcc cgcccgacgc
     4621 agctgcgttg atgacgacca gtgcgccgga ctcgtcgagt gcgacatcgc cgaagcggtc
     4681 gagcacgacc aggcgcacca gatcgttgcg accggcggtc tgggcccacc agaacgcccc
     4741 ctcggcacgg gccgtacccc cggctccggc agcggtcagg atgcgtgtgg cttcctgctc
     4801 gtcgatgggc tggcgcccgg gcacggcgcc gctgtgcaga gcgacggcgc cggtggcgat
     4861 ggctcggacc cgcgagcgtt cggggtcggc ctcgaagcgg acgtcgagcg actcgaccgc
     4921 agcgccggcg gcgagcgcat cctcgcgggc ggcggcgatg agttcggcga tctcggcagc
     4981 cgtcgggttg cggaccgtct gttcccgttc ggtacggatg agcgacaacg cgtcaccgac
     5041 cgacgagatg acctcggcgt gctccggcac cgaacactcg agaccgagga gtttcgcggt
     5101 gtagcggccg aggcccccgg ccccgccgcc gaccgcgacg atgcggggat tcacgaggtc
     5161 gtgctcgtcg gcgacggcgg ccacgagctc ggcaacggcg ttgccggatg cctcgagcat
     5221 ccgccgggcg acctcggcgc cgtcgagccc gagcatggga ccgacgaccg cgaaggcggc
     5281 agtcgcggct tcggtcgagc cctgcgcgta gtcgccggcg tcgatgatgc cggcggcgtt
     5341 cgccgcgcag gtgttggtga gggccaccag ctcgccggac acgagacgga tcccgaggta
     5401 gtcggcggga tcgcccggct tcggggcgaa cagctcgggc gccgcgccgg tgatctccgt
     5461 ggtgtcgagg aagcaggcat agcggaggcc ggcgatgtgg gcgctgcgtg gaccgacgcc
     5521 gtacaccccc atccccgaga agcggctccg ccgggcacgg agcatcgacc cgccggcgac
     5581 cccgatgacc cgcacgtcga cggcgcgcaa cgcggtcgag tggctggcga cccggacgta
     5641 ggagagggcc ggacggccgg cgttgatggc agcgacgttg gtcgacgtgc cgccgacctc
     5701 gatgacgatc ccgtcgtcga cgcgcgaatg ccggagcaca ccggcgaccg acgcggccgg
     5761 gccggagtac agcgttcggg ccgggccctt cctgaagcct tcgagatcgg tggctccgcc
     5821 gtcgccgcgc atgaccatga cgggggcatc gatccccgca gcggcgacac cggcggccac
     5881 gacgtcggcg gtccgcaccg cgatcgggag aatggaggcg ttgagcgccg cggtgacagc
     5941 gcggagttcc aggccgtaca ggcccgacat ctccgtcgag gtgcacaccg ggaggccggc
     6001 agcggcggcg atctcggcga ccaccgcttc gttcgacgag tcctcggtcg cgaacgcctc
     6061 ggcgacgcac gcggcacttg ctccggcagc aacgaagccc tcgacgatgc gcttgatctc
     6121 gccacggtcg agcccgtcgc tcacgtcgac gaactcgcag agcgtacgga gctgcttgcc
     6181 cggcgccagc tcgacgtcgt cgagcgtcgt gcgcttgcgg gacttccgca gatcgggccg
     6241 gcgccccatg ccgatgaccc cgaccgtgcc gacgtcaccc tcgagcagcg cgttgacggc
     6301 ctgcgtggtc gagtgggtca cgagggcgat cgggcgcccg tcgagcgacg ctgcgacgtc
     6361 ggcgacggcc cgcaccaccc cctcggcaac tccgttcggg tgttcgtgcg tggtggcgat
     6421 caccgactgg gcgacgatgc cggtggcgtc gacggccacc gccttcgtga aggtgccgcc
     6481 cacgtcgacg ccgacgcgga ctgccggtga tgcggtcacc gactagttgg cgagcgccgg
     6541 ctcggagccg gtgacgtcgg gaagctcgac gaccgtgtgg tcgccggagt cggtggccgc
     6601 cggacgtgcc tggctgcggc gagggcggat cacgaacatg tacaacgccc cgagcaggta
     6661 caccgcggtc aacgcaccct gggcgatgac accctgggcc gtcgggtagt agccggtcgc
     6721 ctgggccagg aagatctgga ggcggggcca ccccgacaac gcggtgaaac cgatcttgtc
     6781 ggcctcctgc aatgcccgga tggcgttacc caggaaggcg accgacgtgg ccatgatcag
     6841 gaccaccgca accgacatga agacgcggat cgggagcttg cgtccgagtt tgaagatgag
     6901 gaacgcaacg acggccaggg cgacgacacc ggcgaccgcg ccggcggcga tgtacccgcc
     6961 gagccccgtg ccgaacgagt tgagcgcctg gaagaagagc gccgtctcga acccctcgcg
     7021 gtagaccgag gtgaagccga tgagtgtcag cgacgtggcc gacccgaccg acactgccgt
     7081 ccacaccttg gcgcggagga attccatcca ccgccgctgt tcgagccggg cgatgagcca
     7141 gaacgacacg tagaacagca tcgcgacggc gacgagcgcc accaccgctt cgagcacttc
     7201 acgaccgaag ggcagcacgg cgaacacggc gtcgacggcg aagaaggtgg cgaccgtggc
     7261 gacgacggcg aggcccacac caccgatgat gtgcttgcgg tactgcgtgt tcttggtggc
     7321 ctcgaggtac ccgagcagcg ccgacaacag cagcacggcc tcgagccctt cacggaacat
     7381 gatcaggaac gcctggccga atgccaggct cggtgcaccg aggcccttgg cggtgagctg
     7441 gcgttcggcg tcgtcgacga ggccccgcag ctcgatgatc tgggttcgga tctcgtcggt
     7501 cgacgcctcg accttgatgc tcgaacggat ctcggcgaac ttctcctcag cggtgagcgt
     7561 cagctccgga gcggcggcgc ggagcgggat ctcgaccagc tcgaagtggt cgaggtagcc
     7621 actgcgggcg acggcgaggg cttcgtcgcg cttgcccgcc ttgagaaggg ccaaggtctc
     7681 gtcgatcgag tcgcgaacct tgtggagctc gtcgagcgcc gtctcgcggg tcgcgtcgcc
     7741 ctgggtcacg gccgccgggg aggcggcggt ggtcgaggac gcaccggccg agccgccgcc
     7801 gagcagcgtc aacagggtca ggagcgcgcc ggccagcagc gccgcgacgc cgaagcgtcg
     7861 ggcacgatcc gaggcggcga aggcggtcat ccggggtgct tcgccaggta ctcggcgatg
     7921 gcggccgtga tgtcctttgc ccccaacgca cccttctcct cgtcgccctc gacgcccgag
     7981 gacagcagcg acatgctgtc ctcgatgtcg acgtagacgt cgggctcgtt gcgcttgatg
     8041 gtgccctcga tctcctgcca cacgggctcg agctcgtcgt cggccttggc ggcgcgggcc
     8101 gtgtcgggtc cggcggcgac cagttcgtcg accgatgcct gcatcttcgc cagaccgtcg
     8161 gcgacctcgg cgtcggtcgc gacccgttcc tcgggcgcgg tcgtcgtcgg gccgccgtcg
     8221 ctgttcgacg agtcgtcgga ggtgcacgcg ccgaggaagg tcggcccgac caacatggcc
     8281 acgatcgcca ggcgtccgag ggctcgatgc gggggcttcg acatgctgtt ctctccgggg
     8341 atcaggccgg acgtgccaga gcgcaccacc ggtcttaggt atccttaacg tacccacgcg
     8401 ccgagcggcg cagcaaggca cggggtgggc gattcccgga gtgtgaccgg taccctcggt
     8461 tggcgatgga cgcgcgacgc gaggccaagc tccgcaagca cctgggtacc accggcccga
     8521 tcggcgacgg ctgttggatc cagcggccag gatcgccgac cggcccgagt ggactcgttc
     8581 tgttcggcga cacgatcacc ttcgctgcga gcatcgccgt ctccgtcgcc ctggcggcac
     8641 gccgctccca tcgggagcgg cagaggccgg tgaagcccct ccccgaccgc cccctcgccc
     8701 tgatgatctc gggcgacgag gtgctcgtcg gtcggaaggg ccgcgtgctc cgccgctacc
     8761 cgacgagtgt gctcgcgacc gtgcagcgcc cgcgcctgag cacagagatc acactcgacg
     8821 acgagacgtt catcgccggc ggcgtcgagg gcgacgtcct gttgaagctc cgccgctcgg
     8881 cgccgaaacc ctgaccctcg gggtcaaccg atgacgtggg cgtcggcgag ctcgagcgcc
     8941 ttgtcgatgg cggcgagccc cagccggacg tcgtcgggcg atgcgttgca cggcggcacg
     9001 acgtgcaccc ggttgaagtg ggtgaacacc caaactccct gctcccggca cgcggcgacg
     9061 atgtcggcca tgggcttcgc gtcggcaccc gacgcgttgt agggcaccaa catctcgcgg
     9121 gtctcacggt tgcgcaccag ctcgatcgcc cagaacacgc cgagcccgcg ggtgtcgccg
     9181 atcgacgggt gccgctcggc catcgccgcc aactcgggac cgatcacatc ggtgccgagc
     9241 atcgtggcgt gggccagcgt gccctcctcc tggaagatgt tgatcgaggc gacagcggcc
     9301 gcacacgcca gcgggtgacc gctgtaggtg aggccgccgg ggtagacccg ctcgtcgaac
     9361 gtggccgcga tctgctcgga gatgatcaca ccgccgagcg ggacatagcc cgagttgacg
     9421 cccttcgcga aacagatgag gtcgggggcg acgtcccagt gctggctggc gaaccactca
     9481 ccgcagcgac cgaagccggc catgacctca tccgcgatca tcacgatgcc gtaccggtca
     9541 cagatggcgc ggacgccggc gaggtaaccg tcgggcggca cgaggatgcc gttggtgccg
     9601 acgacgggct cgaggatgat ggccgcgacg ttggcggcac cctcgaacat gatgagttcc
     9661 tcgaggtggg ccaacgcccg ctcgcactcc tgcgccggcg actcggagtg gaacgacgag
     9721 cggtaggggt aggggccgaa gtaccggacg acaccgggga tcgacggctc cgacggccag
     9781 cgacgggggt cgccggtcat cgtgatcgag ccgtgggtgg cgccgtggta gctgcggtgg
     9841 gcggcgaaca ccttgtggcg gccggtgtgc aggcgggcca tgcggatcgc gttctccgtc
     9901 gcctcggcgc cgccgttggt gaagaacacc ttgtccatgt tgtcgggggc gacgtcgatg
     9961 atgaggcggg cggcctcgga ccggtcggcg ttggcgtgga acggcgcgat ggtgcacagc
    10021 cggccggccg cctcctggat cgcggcgatc agcttggggt gctggtggcc gaggttcacg
    10081 ttcatcagct ggctgctgaa gtcgaggtac ttgttgtcgt cgaagtccca catccagctg
    10141 ccctcggtgc gggcgatggg gagcgggttg atgagcgcct gcgccgacca cgaatggaag
    10201 acgtgggccc ggtcgtggac gtgggtcgac gtcttgttgt ggtggtcgaa cgagtcgaga
    10261 acttcagcca tgtgacgatg cctacctgtt cgtggggaaa ccgaggtcga tctggctggt
    10321 cgacgggtcg ggccagcgct cggtgaccac cttggtgcgg gtgtagaagt ggatgccgtc
    10381 aggaccgtac atgtggctgt cgccgaacag cgacgacttc catccaccga agctgtagta
    10441 gctgaccggc acggggatcg gcacgttgat gccgaccatg ccgcactcga cgtcgaacgt
    10501 gaactggcgt gcagcaccgc cgtcacgggt gtagatggca cacccgttgc cgaactcgtg
    10561 gtcgttgatg agctgcacgg cctcttcgta ggtgtcgacg cggatgaccg agagcaccgg
    10621 gccgaagatc tcctcggtgt gcatgcgggt ccacggcgtc acgttgtcga cgagggagac
    10681 gccgatgaag aacccgtcgc cgtcgaactc ctgctcgcgg ccgtcacgga tgacggtggc
    10741 accctcgtcg gcggcaacgt cgaggtacga cgccaccttg tcgcggtgct cgcgggtgat
    10801 gagcgggccc atctcgttgt cgggatcggt gccggggccg atcttgacct tgtcgaggcg
    10861 ggcctcgatg gcgtcgacga tcgggtcgcc ggccgagccg acggccacaa cgaccgagat
    10921 cgccatgcac cgctcaccgg ccgagccgta cgcggcgctg atggcagcgt cggcggcgga
    10981 gtcgatatcg gcgtcgggca gcacgatcat gtggttcttg gcgccgccga gcgcctggac
    11041 ccgcttgccg ttggccgtgc cggtttcgta gatgtagcgg gcgatcgggg tcgaccccac
    11101 gaagctgacc gccgcgatgt cggggtgcgt gaggatggcg tcgaccgcct ccttgtcgcc
    11161 gtgcacgacg ttgaacaccc ccgccggcag gcctgcctcg gcgagcacct cggcgatgaa
    11221 catcgacgcg gacgggtcct tctcggacgg cttcaggatg aaggtgttgc cgcacgcgat
    11281 ggcgttggcg aacatccaca tgggaaccat cgccgggaag ttgaacggcg tgatgccggc
    11341 cacgacgccg agcggctggc ggatcgagaa cacgtcgacc ccgctcgcga cctgctcgct
    11401 gaacccgccc ttgagcagat tcgggatgcc gcaggcgaac tcgacgttct cgaggccgcg
    11461 ggcgatctcg cccatcgcgt cagacggcac cttgccgtgc tcggagctga cgatgtcggc
    11521 cagctcggcg cgacggtcgt cgaagatggc gcgagccttg aacaggatct cggcgcgacg
    11581 accgagcgac gtgcggcgcc agtcgccgaa ggcgtccttg gcgaccttga cggcggcgtc
    11641 gaggtcggcg gcgttggcca ggggcagctc ccgggtgatc tcaccggttg ccgggttgaa
    11701 gacgggactc gtgcgagtgg aggtcccggg ggtggactcg ccaccgatcc agtgggtcat
    11761 cacggctatc agctcctgta ttcgttgtgg ggcgagccta ccggtacgaa tcccgctgtg
    11821 tcagggcgaa ggtcgggtcg atatcagtgc cgatccggtg atcgtcccgc cgatcgtcac
    11881 caccggtccc ctaacgtggc cgacctatgc gccggaccct cgccatcgca ctcgtcatca
    11941 ccgccgccgg cacggtcggt tgcggtgacg gcaccccggt gcgagacatc acgcccgatc
    12001 ccacgaccgc aacggcaccg acccttccca cgacgatccc ggtcatcgcc gagacgacgt
    12061 cgctcggcgg agacgccgat ccggcggcac tggcgccggc gacactgccc gacgtgtccg
    12121 atccgatcct cgcgtcgtac cagccagcgc tcgacgggct cgattccgtc ctcggcaccg
    12181 aggggacgac cgccgccctc gtccgcatcc agatcgccac gccgacgcag gcatggagcc
    12241 cggagatgct tgcggcgttg ttcgcatcgt tctgcaacga ctgggaatcg gacgactcgg
    12301 ccggtgaggg cgtcggcccc ctcaccgagc agggccgctc cgactgggcc gcggccatcg
    12361 tcgccccgct gcacctgtcg ccgttcgacg cccgctccgc ggtggactgg gcgctcgccg
    12421 acgtggcgtt caccccgccc tgccagtagt tcgggggacg gactagcggc tctcgatgtt
    12481 gacgaaccgc atgtcggcgt agcgggcccc ggcgacacgt tccttcggcg agatctcgtc
    12541 gaggcgcgcg agatcctgcg gcgagagcgt gatggccacg gccgcggcgt tctggccgag
    12601 gtacgccggc cgcttcgtgc ccggaatggg atgaacgtca tcgccctggg cgtgcaacca
    12661 cgccagcgcg agctgccccg gcgtgcagtc gcgctcgtcg gccatctcct tgaccgcggc
    12721 gaccaagtcg aggttcttcg cgaagttctc gccggtgaag cggggcatgc ccttgcggaa
    12781 gtcgtcgtcg tcgaagtcgg cctcgctcgt gatctgaccg gtgaggaacc cgcgtccgag
    12841 cgggctgtaa ggcaccaggc cgatacccag ctcgcgggcg agcgggacca cctcgtcctc
    12901 gatctcgcgg ctccacagcg accactcact ctggatggcg gagatcggag cggtggcgtg
    12961 tgcgcgacgg atggtgttcg gcgccgcctc ggagataccg aggtaccgga ccttgccggc
    13021 ctcgaccagc tccgccatcg ccccgaacgt ctcctcgatc tcgatggtgg ggtccggccg
    13081 atgcaggtag tagaggtcga tgtggtcgac acccaggcgc gtgagcgacg cgtcgcacga
    13141 cgaccggacg tactccggtc ggccgttcaa cccgcggtag gtggggtcgt ccacgtttcg
    13201 gacgatgccg aacttcgtgg cgagggtgat ctcgtcacgc gatccggggt tggtgcggaa
    13261 atagccgccg agcagcagct cgttggcgtg agggccgtag acgtcggcgg tgtcccagaa
    13321 cgtcacgccg atgtcgcggg cccggtcgag cgtcgcgatc gaggtggcgt cgtcggtggt
    13381 gccgtagaac tcggacatgc ccatgcagcc caggccctgc gccgcggccg acaggccgag
    13441 ggatccgagc gggcgtggtg agagcgacga gaaatcggga tcggccatgg gtccagtcct
    13501 actcgctcgc gaggctgcgg tgtcgccgat cgagcaacgc ccgctcgagc gcgagggtgc
    13561 cggcgccgac caggcacagg agtccgatgt tgagcgcgag ttggagggcg gcaccgccgg
    13621 cctccccggg ccggtcgtgg accagcgcga cggggatgtt ggccgccgcg gggatggtcg
    13681 tgaccgacac gagcacaccg accagggtgc ccgacctgag ctgggtcagc gacaacgcac
    13741 cggcgactcc agcgaggacg gcgacgacca ccgcgtaccc gtcgggggcc gagatgaacg
    13801 acgtgagcgg ccgctcaccg gacaggtagg catcggtcgt ccaaccgaag ccgtcgacga
    13861 tcagggcgag gacggtgacc gctgcgagcg ctgcggcgag gccccacgcc aagctgcgga
    13921 gcccacgacg gaccctcgcc ggtcgacgtc ggtagatgcc gacggcgatg ctcgcgatcg
    13981 ggccgtactc cggcccgacg accatcgctc cgacgatgag gatgggcgag tcggtgagga
    14041 ttccgatggc ggcgatgagc gccgagatca tcatcagggc gaggaagctc ggcgatgtgc
    14101 cactgtcatc gcggctccgc gcctcgacgt cctcccagat cacggcctcg gcggagtggc
    14161 cgggggcgtg cagctcggcc cgacgcgccg cgtccgacag ggaggtgtcg atccgttcga
    14221 tcgagatcga cccgaggtcg tcgatggcga ggcttcgcag gagatcgacg actcggttgc
    14281 ccgactcggg cgccgcatcg aactggacga gatcaccgtc gggccgacgg gccgcaccca
    14341 ccatgctgac gatgttgatc acggcctcat cgaccaggag ggacgacagc agctcatcgg
    14401 tccgatcggg cgggcagacg atacggaagt ggatcacgcc gatgccgccg ctccgacgat
    14461 gccgtcggcg tgctcgacga tccacgacat gagatcgacg agccgctcgg cgagctcctc
    14521 gccgagcggc gtgagcgcgt actcgacgtg gggcggcacc acggcgaagg cgtggcggga
    14581 cacgaacccg tcggcctcga gcgtgcggag cgtctgcgcc agcatcttct cgctgacgcc
    14641 ctgaaccctc cggcgaagct ctccccatcg caatggcgat tcctcggcca gacccaccag
    14701 caccaggaca ccccatttgc tcgtcacgtg gtcgagcacg atccgactcg gacagtcgga
    14761 cgcgaacacg tgatcgggat acgcctcgga gagtttgagc gtcatggtta cctccgggtg
    14821 ggtaccttac ccacaagtgg gtacctatgg tttggaatgc ggtgtcgcct ggccccgttg
    14881 agaccagcgt caaccaccca cacgaaggaa ccccccatga ccacgatcct cgtcaccgga
    14941 acgagtggcc acctcggccg actcgttgtc gagtcgctcc tcgagaacgg cacctcaccg
    15001 ggcgacatcg tcgccaccgc ccgcgacatc tccgccatcg ccgacctggc cgatcgcggc
    15061 gtcacgaccc gcaccgccga cttcgccgac ccggcatccc tcgacgccgc attcgagggc
    15121 gtcgaccgca tcctgctggt gtcgggcaac gagatcggcc aacgcgtcgc ccagcactcc
    15181 aacgtgatcg acgcggcgaa acgcgctgac gtcgaactcc tcgcctacac gagcatcccg
    15241 aaggccgaca cgagttcgct cgggctcgcc gtggagcacc gggcgaccga gcaggtgctt
    15301 gccacctccg gggtcccgta cgcgctgctc cgcaatggct ggtacttcga gaactacacg
    15361 gcccagatcc ccgtccagct cgaacacggc gtggtgctcg gcgcggccgg cgacggcaaa
    15421 ataagcgggg cgtcgcgtgc cgattacgcg gccgctgcgg ccgcggtcct cgtcgccgac
    15481 gaccaggcgg gcaagatcta cgagctcggc ggcgacgccg cgttcacctt ggccgactac
    15541 gccgccgccc tcgcggcggc ctcgggccgg gaggtcgtct accaggacct gacctcctcg
    15601 gactacgccg cggcgctggc gtcgaacggt gtaccgcagg agttcgccga cctgctcgcg
    15661 aactccgacc gtggtgtcgc cgccggcgac ctgttcgtcg acagcggcga cctcacgaac
    15721 ctgatcggac ggcccacgac aacgctcgcc gacgcgatcg cggcggcgtt gccggcctga
    15781 cggcgtcgac ggccggcagc tcacccgaac tcggagtccc ccacggttcg accgtggggg
    15841 actcccgggg ggaggttcag ctctcgtcga cgttggcgtc gtcggtggcg ccggcggggt
    15901 cctcggcgag gatggcgtag agggaacgcc gagcctcgtt gaggatcgcc ttggcccgct
    15961 cgacctggtc gggcgtgccc gccatcgcag cagctgctgc catggcgagg cccttcatga
    16021 ggggcttgag ttcccacatg gggttgccgt cggcgtcggc cgtccagggc tcacggtcct
    16081 cggcgctcag gtcggcgacg acctcccggc catcgtcggt gagcgagaac actcgcttgc
    16141 caccttcctc gacgaccgtg acgagacctt cgtcggccat ctgctggagg gtcgggtaga
    16201 cagcaccggg actgggcttc cagcgggcgc cgctgcgctc gtcgatctgg cggatcatct
    16261 cgtagccgtg catcggcgcc tcgtcgagga gcgcaagcag cgcggtgcgg atctgaccgc
    16321 ggccaacgcg gccgcgcccg ccgcggcgcc cggcgaacgg aggaccgaag cccgggccga
    16381 acggcggccc gaacggcggc ccgtcgcctc gtggaccccg gccgaatctc ggaccgccga
    16441 agtgggggcc acgtcggtcg tcgtcgccga ggaaccggtc gtcgaggtgc tggtggggaa
    16501 gttggtgggg gtgttggtaa cgcatgttgg atctccttgt agatcgctcg tgttcgatca
    16561 agatatatct tgatcgaacg ttcggcaacc ccctttctcc ctggctacgg gccggagcta
    16621 tgagcgatca cagccaaccc cagccgtggg gcggcgatgc cgtctccctc gtcgacgcct
    16681 tccgatccgg cgaacgcagt cccctcgagg agttggacgc cacactcgcc gccatcgagg
    16741 ccagcgatct caacgcgttc tcgttcctcg acaccgagcg ggcccgcgaa gcagcagccg
    16801 acgccgacgt ctcgaagccg ttcggcggcg tgccactcgc tgtgaaagag ctcgacgccg
    16861 tcgccggctg gccgttcacc gaggcgagct tcccgctccg cgaccagatc gccggcttca
    16921 cgctcaccca catcgagcgc ctgcgcgacg ccggcggcgc cgtcccgttc ggtctcacga
    16981 ccgccagcga attcggcggc gtcaacctca cccgcacggt cctcaacggc gcgacgcaca
    17041 atccatggca gcacggccgg accccgggcg gctcgtcggg tggatccgca gccgctgtcg
    17101 ccggtggcct cgtcaccctg gcgacgggcg gcgacggagg cggatccatc cgcatcccgg
    17161 ccgggttctg cggcctcgtg gggctcaaga ccacgtacgg gcgcattcca cgcggcccgc
    17221 acttcgaggt gggagcgctc actgtcacca tcggcggcct cacccgctcg gtccgcgaca
    17281 cggcacgctg gctcgacgtc gccaacggcg ccgaccgcca cgatccacac tcactccccc
    17341 gcgtcgacgg gtgggaggcc gggctcggtg gccacctcca ccacatgaag ggccttcgcg
    17401 tcgcgatcat cgaccggttc gccggcgccg tcgtcagccc tactaccgcc gcagtagttc
    17461 gcgaggccgg cgaacaactc gcccgcgacc tcgagctgac cgtcgtcgac gtggacgtga
    17521 cgatccccaa cgtcggtacc gcctggtcgc tcgcgaacat ggccgggacg ttcgcggcgc
    17581 tgggcgacgc ctggcccgcg tgcgccgacg acctcacccc cgagatgcgc ttcgcgtgcg
    17641 aatgggccgc cggcgccggg tacgggatgg acgcccgcat caagttggag caccggcgca
    17701 ccgccctgtt cgaagccatg gcgacgctgt tcgagcagac cgacctcgtg atcacgtcgg
    17761 ccaaccccga cgtcgccttc ggggccgagg gcccgctccc cgacacgttc ggcggtgtgc
    17821 aggacacccc cgccaacaac ggccggttca cgatcccggc gaacctctac ggcaaccccg
    17881 gcatctcggt tcccgtcggc caggtcgacg gtctcccggt cggcatgcag atcatggcgc
    17941 cccaccacca cgagtcgtgg ctcctcgacg cggcgtgggt cgtcgagcgc gaacgtccgt
    18001 ggccgttgac cgcaccggcg tgaccaattg ggtcgattcg cccatcggtc ggtcgttctg
    18061 gtggacctag cgtgcccgca ccacgacctc cagggggcct catgaagtac gtaccgatga
    18121 agcgagtgct ggtggcagcg gcggtcggag gctcgttcct cctcggctcg gcattcggca
    18181 tcgacggcgt cagcgccggg cccaacgacc cctcggtcgg ccgggcgtcc gtgcgggtcg
    18241 tcgacatgac ccagcccatc agccccgaca tgccgatctg gcccggagac ccggccgtcg
    18301 agttcgacga cctcgcacag atccccaccg acggctactt cctgcgaggg ttcggcatgg
    18361 gcgagcacag cggcacccac ctcaatgcag cgaacagctt cgtcgagggc gggcgatcga
    18421 tcgaccagta caccggccgc gaactggtgg cgcccgcagt ggtgatcgat gtccgaggcg
    18481 aggtgtcgcg gaacgccgac tacgcgctga cgatcgacaa ggtccgtcag tgggagcgcc
    18541 gcaacgggca gatccggccc ggatcggtcg tgctgctgcg taccggctgg gaggaccgct
    18601 ggtccgatcc ggcggcgttc ttcaacgagg acgccgacgg tgccctgcac ttccccggtt
    18661 tcgccggcga caccacccgg tggctctacg accaacgccg catcggcggg gtcggcatcg
    18721 acacccacgg ggtcgacccg ggcctcgacg agtcgttcgc gaccaacgtc ctgacggcgg
    18781 agcgctcgaa gatcgcgttg gagtgcctca acaacatcga ctcgctgccg gcgaccgggg
    18841 cgacactggt gatcggcccc accaagctcg tcggcgggtc cggcgcgccg gcgtcggtca
    18901 ccgcgctcgt gcctcggtga cgacgacgcg atccggcgac ggcgtgccgt gaccggaccg
    18961 tcagcggttc acgatgacgc cggggccgga actgcccgac cgacgaccga gatgcgggtg
    19021 tcgccgatgc ccttcaacga gtagttccga cgcgacggct tcacgacgag gtcgagttcg
    19081 gcggtgagct cggtcgagca ctctcgcgga atgacgaccg tgccgcgacg ggcgatcgac
    19141 gtgagccggc tggccaggtt gaccgtcgaa ccgaagacgt cgccgcccac accgacggtg
    19201 ggaccgacgg cgataccgca gtggatcgtc ggcatgccgt cgaactcggc caggcgatca
    19261 cgaaggtcga gaccgatgtg cacggcctcc gacagcgtcg gggccacgaa catcacctcg
    19321 tcaccgatga gcttcaccac ccggccgtgc gtcggtgcga cgacctcgaa ggcagcggcc
    19381 tcgaacgcgt cgatgacctc ggtcagctcg gccggcgtca gacctttcga gaacttggtg
    19441 aacccggaga ggtcggcgaa cccgaccgcc agcacgtcgg tgtcgtcgtg gccgtcgagg
    19501 gccgggtcga gccgcggatc gaggcgacgg ccgagcgcgg ccagcatgtg ccgcttccag
    19561 acataggtca ccgaccgctc gagcagtttc atgagcgccg gatcgtcctc gtcgagcagc
    19621 ttgcggagcc gctcgcggtg cagcagctcc ggatcggcgt caggcgcctt cgccagcatc
    19681 tcgtccacca tcgcgagctg tgcctcggcg atccgttgga acgacgcacc gagcaggcgg
    19741 gcgagccggg cgatgtcgtc gtcgtcgacg agtcccgcgt cctcgagttc ggcgacccgc
    19801 atgacgaggt cgaggtcgag ctcgttgaac gcgaccgtgt cgtcggccac ctcggggaac
    19861 cccatcgcac gccaccagcg gatcgtgcgt tcggggtccg ccccggttgc tgccgcgatg
    19921 tcggcccggc gcagcgtcga cggctcgagt ccgaccgatc ggtggaggtc catggcccca
    19981 tgcttgcagc ccccaggggt ggccacgagc gtgtggggcc cctcgagcgg gtgtagaact
    20041 tgtcccggta tcgaacgtcc aaaaggggga catgtggaga gtcgcgctgc agtgctgtgg
    20101 ggaatcggcg agccgtggaa ggtcgaggac atcaccatcg acgaacccgg ccccggcgag
    20161 gtcctgctga agaccaaggc cgccggcatg tgccactccg acgagcacgt cgtgaccggc
    20221 gacatgttga tgccgcacta cccgtgcatc ggtggccacg aaggcgccgg cgaggtgctc
    20281 gccgtcgggc ccggggtcac gagcgtcgct cccggcgacc acgtctcgat gtcgttcatc
    20341 ccgtcgtgtg gccgttgcaa gtggtgtgtg tcgggccgcc agaacctgtg tgacctcggc
    20401 gcccagttgt tcgacatcgg catgatcacc gacgggcgca tcgcccacaa ggtcggcgac
    20461 acgacgttgg ctcgcatgtg ccaggtcggg tcgttcgccg agcatctcct cgtcgccgaa
    20521 tcggcggtcg tgaaggtgga acccgacatc ccctggcacg ccgtggccct cgtgtcctgc
    20581 ggcgtcgcca ccggcttcgg ttcgtcggtg caccgcgccg aggtgaagcc cggcgacaac
    20641 gtcgccgtca tcggttgcgg tggcatcggc atgaacgcca tccagggcgc gaagatcgcc
    20701 ggcgccaagc gcgtcatcgc catcgacccc gtcgagttca agcgcgagaa ggccatggag
    20761 ttcggggcga cccacaccta ctcgtcgatc gaggaggcgc aggccgagat cagcgccctc
    20821 acgtggggcg agatgtgcga caagacgatc ctgaccgtcg gcgtcatgac cggcgacctc
    20881 atcgagccgg cgctcggcct cacgtcgaag ggcggcacct gtgttgtcac cgccgtcgcc
    20941 aacatgatgg agaacgacgc caagctcaac tcgttcatgt tcgccatgat gaacaaggag
    21001 ctgaagggct gcctgttcgg ctcgggcaac ccgcgcgccg acatcccgat gctgctgtcg
    21061 ctctaccgcg agggccagct caagctcgac gagctggtga ccaagaccta cacgctcgac
    21121 cagatcaacg agggctacca ggacatgcga gatggcaaga acatccgcgg cgtgatcgtc
    21181 ttcgattgat ggcggcctga tggtttcgta ctcccgcgag ctcgtcgacc gcctcgacgg
    21241 atcggtcgtc ggtcgacgat ccgagatcga acaggtggtt gccgggctcg cggcgggacg
    21301 gcacctgctc ctcgaaggtc cccccgggac gggcaagtcg acgctgttgc ggcgcgtcgc
    21361 cggagagctc gaccgcgggt tctgcttcgt cgagggcaac gccgagctga cgccggcccg
    21421 cttgatcggc acgttcgatc cggcggcggt gctcgaggcc gggtactccc ccgacgtctt
    21481 cctcgacggt cccctcgtga ccgcgttgaa ggacggcgcg ctgctctacg tcgaggagat
    21541 caaccgcatc cccgaggaga cgctcaacgt gctcatctcg gtgatgagcg aaggcagcct
    21601 gcaggtcccc cggctcggcg cgatcgacgc agccgaggga ttccgcctga tcgcggcgat
    21661 gaacccgttc gacgcggtcg gcaccgcccg gatctcgggc gcggtctacg accgcgtgtg
    21721 ccgcctcggc atgggctacc aacgcgccga cgaggagatg ctgatcgtcg gcaaccaggc
    21781 ccgcaccgcc gcagcgaccg tcgggcccgg cttcatcgtc caggcgaccg agatggtccg
    21841 ggcgacccgc gagcatcccg atctccgaac cgggtcctcg gtgcggggcg cgatcgacct
    21901 gacgatggtc gccgccgagc tgcgggtgct ccgcgacctt ccggccaccg atcccggcgt
    21961 cacccgcgac gcggcgcagc tcgccctgtc gggccgggtg cgagtgcgcg acggatgcgg
    22021 tcgcaccgca gccgagatcg tggacgagct ctgggcgatc ttcttcgccc cggaggccga
    22081 cgacgatccg gagacgggtg aacccgacgg cgacgactcc acgggaaaag gccagcgcca
    22141 ccacctcagg gcggtggcgg gtccgacgag gtagccgaag gcgacgacgc ccgacgcgcc
    22201 gtcgccgacg catcgcgtcg gacgacctcc cgccgcgagc tggagcgaca cgagatcttc
    22261 gacgaggtca gccctgaggt gggcgagctc gacgaacgcg ccttcgagcg ggccatggac
    22321 gaggacctcg acgaggcgct gaccctgctg gccgacctca ccggcgccac cgacgagcgg
    22381 ctccgcgagc tggcgcgcac cctggccggt cgcgtcctcg tgaacatcgc ccgcaccggc
    22441 gtgccccggg accgcggcgt gggccggatc cgcccgaggc gggcatcgtc ggccgacggg
    22501 gacgtcgacc tcgacgcgag cctcgaagcg atcgtggcgg cccgcgccga acgccgacca
    22561 cccgcggtcg acgacctgac ggtgcaggcc tgggcgcggc ccgacaccgc gctgtgcctc
    22621 ctcgtcgacc gatcgggttc gatgaagggt gatcggctcg cagccgccgc tattgcggcc
    22681 gcagccgtgt tgtaccgcca cggacgggac tgctccgtcg tcgcattcgc gagcacggcg
    22741 atcgtggtga aggcgcagga cgaacgccgc gatgccgacg aggtcgtcgg cgacctcctc
    22801 cgcctgcgcg gccacggcgt caccgacctc ggtctcgctc tgcgcacggc gcgtcgccag
    22861 ctcgaacggt cgtccgcggg ccgacgggtc accctgctcc tgtccgacgc ccggtcgacg
    22921 accggcgacg acccgaccgc cgatgccgcc gcgctcgtgg cgctcggcga cctcgctgtc
    22981 atcgcgcccg agggcgacac cgccgacgcc gaggcgttgg ccacatcggt gggcggtcgg
    23041 tgggtgtcgc tgagcggacc gtccacgatc ccgggagcca tcgccgaggc gctgctgcag
    23101 tcctgaccgc gctatccagg aaacgctaca caactgttgc gttttgcccc tcgggccggt
    23161 agaccttccc ggagcactga gcagcacgaa aggcccccca tggcacccaa gacccccctc
    23221 gtcgcgcccg gcgacgcgac ggccgcatcc tccggcaccg agcccgagga cacgtacggc
    23281 atcgaccccg ccatctacca ccgccgttgg ctgatcctgt cggtcctctg catcagcctc
    23341 gtcacgatcg tcgtcgcggt cagctccctc aacgtcgcga tccccacgat catccgcgag
    23401 ctgggagcga actccaccga gtcgctctgg atcatcgagg cctacgccct cgtcttcgcc
    23461 ggggtgctcc tccccgccgg cgccctcggc gaccgcttcg gccgcaagtg ggcactgctc
    23521 ggcggcctcg tgatcttcgg cggcatggcc ctcgtcgcca gcttcgccgg atcgccggcc
    23581 cagctgatcg ccgcccgcgc cgtgatgggc atcggcgccg ccctcatcat gccggccacc
    23641 ctctcgatca tcaccaacgt cttcccgccc cacgagcggc agcgagcgat cgcgacgtgg
    23701 gccggtctcg ccggcgccgg cggcgccatc ggccccctga tgagcggcat cgtgctgaac
    23761 gccggttggt ggtggggttc ggtgttcctc gtgaacatcc ccctcgtcgt tgtcctcatc
    23821 ggcctcgtgt ggttcctcgt gccgaactcc aagaacccca atggtgaggc gctcgatcct
    23881 cccggggccg tcttctcggt catcgcgctc ggcggcctcg tcttcgccat catcgagggt
    23941 cccgagtggg gttggctgag cggtggggtg ctcggcacat tcgccctcgc gctcctcggc
    24001 ggtgcggcgt tcatcgcctg ggaacttcgg gccgctcacc cgatgctcga tccgcgcctc
    24061 ttcaaactcc gcgggttcgg catgggcagc ctggccatca cgctcgcctt cttctgcatg
    24121 ttcggaacgt tcttcctgat ctcccagtac ctgcagttcg tgcacgggta ctcggcgctc
    24181 ggcagcgcgg tgcgcacgct gccgtccgcg gtcgtgatga tcatcgtgtc gcctcgcagc
    24241 ccgaagatcg tggcgcggtt cggggtgcgc aactgcgtcc ggggtgggtt cggcctcatc
    24301 accctcggtt tcgtcggcat gtcgaccctc ggggtcacga gcgcctactg gcacctcgcg
    24361 atcttcctct gtgtgatgtc gacgggcatg gccacgctga tggcgccggc gagcggcatg
    24421 atcgtgtcgt cgttgcccct gtcgaagtcc ggggtcgggt cggccgtcaa cgacgtgacc
    24481 cgggaagtcg gtggcgcgtt gggcatcgcc atcatgggat cggtgttggc gtcggggttc
    24541 agctccaaga tggcggaccg gatcaaggac cttccgatcc cggccggtcc ccgcgagttg
    24601 atcgaggact cgatcggcaa ggcgttcggg gtgtccgacg aggccgtccg atccggcctg
    24661 atcccgcggt ccgccggcga actcatccac gactccgccc gcgaggcatt cgtcaacggc
    24721 agccgcatcg ccttcttcgt ggcggccgcc gtcgcggcgg tcggtgcgct cgtcgccggc
    24781 aacctcatcc ccgacgagac accggagcac ggcagcgtcg tcgatccggt tccggcgtcg
    24841 gccgggtgat cggctcgtga ccgcagcgga gcggtcgggt ctcgatcctc gcgtcgcgcg
    24901 gagtcgcgcc gcggtgatcg aggccgccct cgacctcctc ggcgacgccg gctacggcgg
    24961 gacgagcatc gacgcgatct cccgccgctc cggcgtggcc cgcacgacca tctaccgcca
    25021 ctggcgctcg ctggccgaga tcgtgcacga ggcggcgatg accacggtcg gagtcaaagc
    25081 cgtccccgac acgggggatg cccgtgccga tctccgagcc cacctcggcg agctcgccgc
    25141 gaagctgaac agcggggcgt ggggacgcat gctgcccgtg ctcgtcgacg cggcgttccg
    25201 ggacgaggag atcctcgagc tccagcgccg ctccaccgcc gaacgcaagg cggcggcgat
    25261 ggccatcgcg gcgcggggtg tggaactggg ccagatccgc gacgacgtcg acctggtcct
    25321 ctgcggggag atgctcgtcg gtccgatctt cacgcgtcac ctcgtcaccc acctgccgat
    25381 caccgaggcc ttcctcgacg acctgatcga ggccacgttc cgcatgatcg gcgcctgatc
    25441 tccggcgccg atcccgacgg ccgcggttgg gtctcgtgtc ccccgggtac atcggggcat
    25501 ggagatcatc aggaacgacg acggcacatt gacggtaccc gtggaaccgg accggcaggc
    25561 ggtcgaggcc gccgccgaga gcggggagac cctcactgcc accacgatga cgctgcatcc
    25621 gggcgaaggt ggttacgacg aggcgctgtc cgaatgggat ctgcaacagc acgacggcca
    25681 ggccgccgtg tcgaccgcat cgggccgcga agcggcgatg cacgtgatcc acgaggtcgc
    25741 cgccgacccc gacatcgaca tcgccgacgc cgtcgaggag ttggacgatc ccgacgccag
    25801 cgccgaggcg ttgcgccacg tcctcgtcgg gggtgtccat tccgtcgagg acttcgcggc
    25861 cgaggtggcc gaccacgaag gcggtgacgt gctgcctccc cacgaggtga cgaagatcat
    25921 cggcgaggtc ctcgcggaga tcgacagctg aggttcgtgc tcgctgagga tggtgcgcca
    25981 gcgcgcccga atcgggccgc ggcgacacca tcctctgcag cgcgtcggtt cacgggcagg
    26041 cgccgacgct gggcaccgct catctcgtag gtcccgcgtg ccctcacacc gaatgatctg
    26101 acgaaccacc tccggatcgg ccaagatcag accgggaccg gttgccgccg gtcccggagc
    26161 gccggaagga ggccgccatg aaggatgaac tcgtcgcggc cctgcacggg cctgccgagc
    26221 agctcgcggc gctcctgtcg gagcgtcgtg acgagctgcc cgaggtgtgg gcgctcaaga
    26281 cgctgtcgga ggatcccgtc ggcgacctcc acaaggacaa cttcgcccac acggtcaagg
    26341 tcgtggagca gtgtggagac accggcgacg tcacgttgct ggcggcgctc ttccacgacg
    26401 tgggaaagcc gctgactcgc cgcgtcgagc gaggcgaggt cacgttccat ggtcacgaga
    26461 tcgtcggggc caagctgatc cgcaagcggt tcgccgtgct cgagctcgat cccgaaccgg
    26521 tgatccggac gatcatgatc gcggcgcggg cccagtccta cctcgactcg tggaccgact
    26581 cgtcggtgcg gcgcatccgc ctcgatgccg gcgacgactg ggaccacgcg atccgcctcg
    26641 cacactccga cgtcacctca cggcacgcat atgtccaccg tcgtgtgcac cagaccgtcg
    26701 acgcgctcac gacccgatcc gacgacctgc tggtcgagga cgcccgcaag gccgagcgac
    26761 ccgtcctgaa cggtgacgac ctcgccgagc tcggcgtgcc ccgcggtccg gagatggggc
    26821 gcctgctcaa gtggctgctc gaggagaacc gggccggacg cctcaccgag cggcccgagg
    26881 ccgaggccgc gatccgctcc cacttctgag cgccgcaccg cgccggatca ggaacgcagt
    26941 tccggcctcg tcgcgatcgc atcgatcgcc tcggggttgg ccagcgcgct ggtgttgcgc
    27001 acctcacggc cgttgaccgc gtcggtcacg gcgagctcgg cgagtttgcc cgaacgcgtc
    27061 cgcggcaggt cgtcgacggc gaggaccacg gccggcacat ggcgcggcgt gcaccccacc
    27121 cggatggtgt gtttgatctc ggcggacagc tcgtcgtcga gcgccacgcc aggagcaaga
    27181 cgcaccagga gcacgatgcg agagtcgtcc tcccagtcct gaccgaagac caggctctcg
    27241 acgacggagt cgacctgctc gacgcgccgg tagatctcgg cggtgccgat gcgcacaccg
    27301 ccggggttga gcgtggtgtc gctgcgaccg tgcacgatca tgccgccgtg ctcggtccac
    27361 gacgcgaagt cgccgtgact ccacacaccg gggttcttgt cgaagtaggc ggcgcggtag
    27421 cgcgaaccgt cgtcgtcggc ccagaagccg agcggcatcg aggggaacgg agccgtgcac
    27481 accagttcgc cgcgctcgtc gaccccggcg ggtcggccct gctcgtcgaa gatgtcgacc
    27541 gccataccca ggccgggacg ctggatctcg ccggcccagg ccggactcgt cggatcaccg
    27601 gcgacgaagc aggcgcacag atcggtgcca ccggagatcg acgcgaggtg cacgccggca
    27661 gggaacgact cgaccgccca ggtgttcgtc tccggtgaca ggggcgatcc ggtcgacgcg
    27721 atcgtgcgca gcgacgacat cgccgcgtgt tcacgcggca cgaagccgtc cttgcgcagc
    27781 gagtcgagat acttcgccga cgtcccgaag aacgtcaggt cctgggcctc gatcagatcg
    27841 gtcatggcag ccgcgccggg gtggaacggc gagccgtcgt acaacaccgc cgtcgcaccc
    27901 gacgccggca ccgaggccag ccagttccac atcatccagc cgcacgtcgt gtagtacgcg
    27961 acgtgatcgc cgggaccgat gtcgcagtgc agctggtgct ccttgaggtg ctgcagcagc
    28021 actcccccgg tgcggtggac gatgcacttg ggcacaccgg tggtgccgga gctgaacagc
    28081 acgtaccagg gatggtcgaa cgggaaccgc tcgaactcga acgaggcaac ggcctcggcc
    28141 ggcgccgtgg tgaggaaccg tccccaaccg atggcgtcgg ggatcgctcg ccattcgccc
    28201 accgggtccg cgccgtagcc ctggcggtgg cccacgacga tcacctgtcg caccgacggg
    28261 agtgccgctc ggatggtcgc caggcgatcg acgcaatcga gcgacttgcc gccgtaggcg
    28321 tagccgtcgg cggcgaacag gagcacgggc tcgatctgcc cgaaacggtc gacgacgccg
    28381 tcgacgccga agtcgggcga cgtcgaactg aacaccgccc cgatcgatgc cgcagcgagc
    28441 atgaccatca tcgtcgtggg aatgttgggc atccacgccg ccacccggtc acccggctgc
    28501 acaccagctg cacgcatggc acacgcgagg tcgacgaccg tgcgacgaac ctctgcccgc
    28561 gtgaccgcgc tcccctcgcc ggagcgctcg tccacgccga cgtacatcca ggactcctcg
    28621 tcggcgcccc gaccgtcgag gatcacttcg gccacgttga gccgggcgtc ggggaagaac
    28681 cgtgcctcgg gcatcgtggc gccgaggtcg agcacccgct cacctcggtc accgatcatg
    28741 ccggtccagt cccacaacgc ggcccagaac gcgtcgggct cggccaccga ccagcggtgc
    28801 agctcggggt agtcggcgaa gccatgcgcg tcgcggaaac gggccatcgc cgaggacgca
    28861 gcgcccgccg ggtccggaac ccagagtggt tcgctcactt gacccgccca cgccacccgt
    28921 gacggagctc ctgcgtcgca ctcacttgac ccgcccacgc cacccgtgac ggagctcctg
    28981 cgtcgcactc acttgatcag gcccaggtgg acgcccgaca ggaaccgctc gacgtactcg
    29041 gtcgtgcgca ccgaacgcgg cgacggaaag atgtcgaacg cgtggttcgc gccggcgagc
    29101 tcggcgtaga cgacggcatg ctccgatgtc ttcgccatgg cctcggcgaa cggtcgggcc
    29161 tgctcgacgg gaacgagcgt gtccggggtg ccatggacga tcatcatggg cggagcatcg
    29221 gcgtgaaccc ggtgcaacgg cgagaaccgg cggtagcgat cgaggtcgtc ggccatcttg
    29281 gtgccgacca cgacgtacct caggaagtcg tcgaagccgt tcgccatggc gtggttcgag
    29341 tcctccaggt cgtacacccc gtagaacggc accgccgcct gcacggtggt gtcgtcgtcc
    29401 tcgaagccgg gctggaactc ggggtcgttg gcggtgagcg ccatcatcga tgtgaggtac
    29461 ccaccagcgg atccaccggt gacgcagatg aaatcggggt cgaccccgag gtcgacggca
    29521 ttgcggcgaa tccaggcgat ggcccgcttg cagtcgatca actgctcggg gaacttcgcc
    29581 ttcggcgcga ggcggtagtt gatgttgaac ccgacccagc cgttcttcac catgtggtgc
    29641 aggagcggca ctccctgctg gttcttgtcg cccaggatcc agccgccgcc atggatctcg
    29701 acgatcgcgg gacgcgggac gctccccttc ggcgcctcgc cctccggcac ctgctcgtgg
    29761 gccgccgcct caacccgctt cggctggtag acgtcgagct tgttgcgcca ccgggcgtcc
    29821 tcgacatagc cgacgttacg catccgcttc gtggtcgatg cgccggtgaa cagcgggatg
    29881 atcgcaccgt tgcgaaccga cgacggtcgg gcagcaaggg tctcggcgtc gaggtaggga
    29941 gcgagcacgg cttcgaactc ctcgtcggtg cgccgaccct gctggatgag gccgaccagg
    30001 ccggccatcg tgacggcgtc gagcgccagc gcgaccttgc cctgggtcgt ccggtatcga
    30061 ccgaggcgcc acgcccggac ggcgtggacc gcccgtcgga ggagcagcag ccccgcggcc
    30121 tcggtcgtga tccatccgga gaagaacgac ggaatggtga ggacccggct gcggatccga
    30181 ggcttgcggg cgttgacgat ggcgagggcg ccacccacgg cagatcgaag cacgggttcc
    30241 atctccgtat cttggcccac cgtcgttcgg gccgccgcac caacgattcg tgaggcgatc
    30301 gcctcatgtc tgcagggcgc ggcgcgatcg caagtgcgac agaatggccc tcgacttttc
    30361 gccacggggg attgatgcaa cgactgtccg gactcgacgc caccttcctg tacctggaga
    30421 cgcccagcgc gcacatgcac gtggcgatga cgggcatcta cgacacgtcc acgatgcccg
    30481 gcgggtactc gttcgaggcg ttccgagatc acatcgtcgg gcgcttccac cgcatccccg
    30541 ccttcacccg gcgactcgtc gaggtgccgt tccagctcca ccaccccgtg tggatcgagg
    30601 atccgcactt cgacgtcgac taccacgtcc gccgcatcgc tgtaccggcc cctggcggtc
    30661 gccgcgagct ggccgaggtg gctgcgcaga tcgcgtcgat cccgctcgac cgcacccgtc
    30721 ccctctggga gatctgggtc atcgaaggtg tcaagcagaa ccggatcggg ttcgtcgcca
    30781 agatccacca ctcggcgatc gacggcgcgt ccggcgcgga gatcatgacc gagctgtacg
    30841 acctcgagcc gaacccgccg gcccggccgc ccgccgagcc ggccgaaccc gagcacgtgc
    30901 cgagtgatct cgagctcgtc acctacgccc tggcctcgaa gctccgtcgc agccgcgaca
    30961 tggtgcccct cgtcgggcgc accgtgcaga gcgtgtcgaa cgtcgtcagc aaccgccgca
    31021 accccgatgg tgccgtcggc gccgtgccgc tcacggcgcc gcgtaccccg tggaacggtg
    31081 cgatctcgcc caagcgcagc gtcgccttcg cccgcatccc cctcgacgag ctcaaggccg
    31141 ttcgtcgggc cctcgatgtc aaggtcaacg acgtcgtgct ggccgtgtgc gccggcgccc
    31201 ttcgcagcta cctgatcgct cacgacgaac tgcccgacgg cccgctcttg gcggtgtgcc
    31261 cggtgtcggt ccgatccgac gaggaggtcg gggagggcgg caacaaggtc tcggcgatgt
    31321 tcacgtcgct cgccaccgac atcgacgacc cgctcgagcg tctgcgtacc atctccgact
    31381 cgaccgtcgg ggccaaggcc gagcacaacg ccgtcggggc acgcatgttg accgactggg
    31441 gcgagtacgc agcgccccgc accttcgcga tggcatcgcg gctgtactcg tcgatgagcc
    31501 tggccgacca ccaccgaccg atccacaaca tcgtgatctc gaacgtgccc ggccccccgt
    31561 tccccctcta tctcgccggg gcggaattgg tggcggcgta tccgatgggc ccgatcatgg
    31621 agggcgccgg cctcaacgtc accgtcctca gctatcgcga cgctgtcgac ttcgggttca
    31681 tggccgaccc ggcgctcgtg cccgacgtgt gggagatggc cgaccacgtc caggaggcgt
    31741 tccgcgagtt gaaggacgcc gccggcatcg gggtggaatc cgccgaaccc gcgccagcag
    31801 cgccggagcc ggcccccgag ccggcggcct cgcgacgtcg ccccagcgtg gtccagaagt
    31861 cggcacgatc ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacgggct
    31981 gacacccggc cgccgcggcg ggatcgtggt gcgtttccct cacaagcgcg gacccgcgcg
    32041 ccgacgagga gaacatgtcc tcaacgatcg cgccgccggc tcgcccggtc cgccctgcca
    32101 gcgggagcgg aacgcccggt cccgacgccg ccacatcgcc ggaccgatcc ggcgcactcc
    32161 gcctccccga cctggcggtc gcgctcttcg ccggtgtgtg gttgacctcg agctgggaac
    32221 ggatcggcgc accactcggc ctcagccacg acgggcagaa cgccggagtg ttcggctccg
    32281 ccgcacggaa gctcgtcgac gacccgttcg gctcgcacct cggatcgcgg ctcgccaacg
    32341 ggcacggcgt ctactccaac cacccgccgc tcctgaactg gttgctcggc atcgggcacg
    32401 ccgtcacggg cgacaacccg gcgggcttgc gggttccgat ggtgatcgcc gcgctcgtgc
    32461 tcatcgccgc gacctaccgc ctactccgac tgttggtgtc ctcaccgtgg gcggcggcga
    32521 tcgggacgat cgcggggctc gggctcccga tgttcttcgt ctacggaagc atgcccgaca
    32581 ccccgatgct cgggctgccg ttcggtgtgg tgctgctcgg ccaggtcgtc gccgccgaga
    32641 cgacgccacc caaacggtcg tggctcgtgg tcgccacggc cgccggctgc gcactcgccg
    32701 ggtgggaggc cgtgttcttc gccgtcatca tcgccacggt cgcactcgtg cgggggcgcc
    32761 gcggcgaccg aacctcgacc acgaccggac gagcggtgtt caccggactg gcgatcggcg
    32821 gcgcggtcac gatcgcgtgg atcgcctggg cggatggcgg actgacccga ctcgccgacc
    32881 agttcctgtt ccggtcgggg gcggccgacg tgccgttcac cgtcggcgag gcggtcgtcg
    32941 cgcagatcca gtacctcgcg gatgtgtacg gcatcatcct cgtgctcgcg atgttcgccg
    33001 cactcccggc gctgaggatt ccagcggtac gccggcatct ccccaccgtg gtcggggtgt
    33061 gcctcgtcta ccacgtcggc ttctggcagg gggcgacgta ccacgactac tggtcgtact
    33121 ggtggcaact cccgatggcg atgtgcttcg ccg
//
LOCUS       DAOSOA010000006        32034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_1422_length_32034_cov_6.244973, whole genome shotgun sequence.
ACCESSION   DAOSOA010000006 DAOSOA010000000
VERSION     DAOSOA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 32034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32034
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1422_length_32034_cov_6.244973"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKY13_01035"
     CDS             complement(<1..832)
                     /locus_tag="PKY13_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78532.1"
                     /translation="MAVVHRVLPESPIVTLDDHLANGGGVGLAAARELAPSAVVALLD
                     DAGLRGRGGAGFPTGRKWAAVIENLAADAPATVVVNGAEGEPGTFKDRSILRTNPYAV
                     LEGALIAAHAVSADRVIVAVKASFHPEADALRDAVASLVAARWCDDVQVDVFVGPSAY
                     LFGEETAMLEALDGRPPFPRIAPPFRRGVDEVVAGAVDLTSGSGLSAHVEMSEPTGAT
                     DAPPALVDNVETLANVTFILAQGVEAFRSLGTSESPGTVVCTVSGSVVAPGVAEVEMG
                     T"
     gene            898..1860
                     /locus_tag="PKY13_01040"
     CDS             898..1860
                     /locus_tag="PKY13_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78533.1"
                     /translation="MRISMTHRGARSTIAAAVAMLVVGGCGGTDDAADPPTPTSTAPA
                     STTTTTTTPSVVSRRPFTTEHSERTYQRTAADGSTRDLLTEVWLPVGNRPFPLVAFAH
                     GMSGHPRKFHELFEAWAAAGYAVVAPRFPLSAADAGGSLADSVADAPQQALDLEFVVA
                     RVLAESADPTDPLHGHIDPDAVAVAGLSLGGGTALLASYSGCCPGLTPSVVLAFSPVP
                     FATEQAADAAPLFLMHGQKDTTLPYSGSRDLFDTAKNRRWLVTMPDAGHAAPYEDDPS
                     AQDDLVRTVSLDVLDQFLLGDDDGVSRAESSVAASGLATIESAG"
     gene            complement(1871..2806)
                     /locus_tag="PKY13_01045"
     CDS             complement(1871..2806)
                     /locus_tag="PKY13_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT78534.1"
                     /translation="MSLISELEAQLTHTAALVAPSTVSVGRDHRGAGVVVAPGKVLTN
                     AHNLRDRTVQITFDDGRVEQAKVVGADPGHDLVVLDVDTGDAPALEWADRAPGLGAFV
                     VAAARDGAGVRVTLGTVSQPSRSFRGPGGRPIALALEHTAPLARGSSGGPLTDLEGRL
                     VAVNTHRLGKGFTAAQVASDALRSTVDDLSAGRSRRPLRLGVEVAPSHVAHKLRAAVG
                     LDQRDGLLVRGLVDGGPAAVAGVRIGDLLVSAGGVDLVRPDDLFTALSSHDAGDPLVV
                     AVVRGSEELTLDVRFVADTADTAADAAADAVTDEA"
     gene            2894..3310
                     /locus_tag="PKY13_01050"
     CDS             2894..3310
                     /locus_tag="PKY13_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT78535.1"
                     /translation="MEPAAPLAFAVERIGDRWTILIVDALMSGPRKFGELEADVTGIA
                     PTVLTKRLRQLDADGIVVGRPYSERPLRFSYELTGPGRALAGAIQLLRDWGASHTGDE
                     DAAMHTACGSPLVTRLWCPSCETVVGDDDASENVSL"
     gene            complement(3326..4075)
                     /gene="aqpZ"
                     /locus_tag="PKY13_01055"
     CDS             complement(3326..4075)
                     /gene="aqpZ"
                     /locus_tag="PKY13_01055"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HOT78536.1"
                     /translation="MDQPLVKRCGAEFFGTLWLVFGGCGTAVFAIITRGDLDTVGLVG
                     VSLAFGLTVLTGAYAFGHISGGHFNPAVSVGLAVAKRFDWKDLPADVASQVLGAIAAT
                     GLIWAIVSAMPGGFDKQYGKLASNGFGVEHGYYSLGGVLLAEAFLTFMFLLIILGATH
                     TKAAAGFGGLAIGLALTLIHLISIPISNTSVNPARSTGPAIFEGGTALSQLWAFWLAP
                     IVGAAIAGLVYVWLRPEDAANSGPVSTPAEA"
     gene            4206..5231
                     /locus_tag="PKY13_01060"
     CDS             4206..5231
                     /locus_tag="PKY13_01060"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HOT78537.1"
                     /translation="MDFGLRNTHRRRALCAVLVLGLAPVSAAAAGDTPAAPSIPSVAV
                     AHADVLRSEQHLIEVTGTLDAARLELDSLNTRSAELVDQIAAARAELRDRAVGAFVSS
                     EEQDETVLYLVGGQDLFESSARIRMMTHSAEVSSAAFRHYNDLKASVDPKVVELGERI
                     QQLEQDVADANDAALAARAIEAEAERQAEQRARAERAARAAAEAAAAAARSTTIPTTV
                     PAATVVADRAPSEPAPAAAAPAAAPTAAPVPLATVPLPAVPAGGPSEAQWAALRNCES
                     SGNYRAVSSSGKYRGAYQFDYRTWAGLGGTGDPAAAPPAEQDARAKLLYSQRGWRPWP
                     VCGRFLR"
     gene            5301..5996
                     /locus_tag="PKY13_01065"
     CDS             5301..5996
                     /locus_tag="PKY13_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT78538.1"
                     /translation="MSAAEPLAHVLVAEDDRSVRDSLARVLRFEGYEVEAVNDGAEAL
                     EAVTRRLPDVVVMDVMMPNVDGLTACRMMRDRHPALPILMLTARHEVADRVAGLDAGA
                     DDYLVKPFALDELLARLRALVRRTSVSGSSESLVLDDLTLDPLRRQVTRGGRAIDLTK
                     TEFDLLELLLLNAGIVLSRETIYDRIWGFDFETGSRSLDVYIGYLRRKTEDGDRPRLV
                     HTVRGVGYVARAT"
     gene            5993..7423
                     /locus_tag="PKY13_01070"
     CDS             5993..7423
                     /locus_tag="PKY13_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT78539.1"
                     /translation="MSLRLKIAAALAALAGIIAALAAGGAYVTAARQLDRTIDETLLE
                     RASAAVNDTSRPGRGEPGNRQPFMPGRARCPDVNVLQTFTAAQVIDDGEVRQCIVGGA
                     TIEPSERDLAIANGTDFHGDDDHDGDADANYAFSRARIGNETVRVITMAFPEGGAIQL
                     ARETGEADRVLDGLRIRLLLIALTGIVVAAAVGFALATRIVRPIRRLTTTAEHIAATR
                     DLTTPVPAAGSDEIGSLSASFTTMVESLAESQDQQQRLISDASHEMRTPLTSLRTNLE
                     LLARAELAAPGSLSAEDRRAVIDDLQFETGELTALLTELVELATDRAGGEESVPVDLT
                     VLTSGVAERARRRTGRVIEVVGPDGADAGTGVVVTVRQHMIERAVSNLIDNAVKYSPD
                     GAPVDVIVDGTRVEVRDHGNGIADADLPRIFDRFYRATEARTAPGSGLGLAIVNQIIE
                     GHGGRVFAGNHGGGAAVGFELPTPPA"
     gene            complement(7477..8652)
                     /locus_tag="PKY13_01075"
     CDS             complement(7477..8652)
                     /locus_tag="PKY13_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78540.1"
                     /translation="MRTTSWGAAWIERMVAARTFEGTELSRGRGLAARGAVDEFTVEP
                     GSVGGVVRGRSSLERRVRVGVRRFTDEQWATVRATLAGSSRLVAAVLEGALPEALHDR
                     LEAVGCGLLPGVRDVSIDCSCGNLAESCVHAAAVVCRLAEALDADPLLLVMLRGGDRS
                     ALVQGMVPVSSGRDRERAAADQRVSAIPGRPADPTMSAATAWKRRLDEIPTIDRRRRA
                     PGRPRVGAAPPSAELGFDQAGLDRLVRDAAARAGALLGAPASGAGLDLDLIADAVRRS
                     ASGTLAAPDSVSAVVAAAAGCPPESAPALTEAWRHCGPAGIDVLLRPRRLGPDELELI
                     AATFEIEVKARSTGAVLPGGRQIRRADDGTWIVLASDDVAGWRIVDAAADLDDLTID"
     gene            complement(8667..11525)
                     /locus_tag="PKY13_01080"
     CDS             complement(8667..11525)
                     /locus_tag="PKY13_01080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT78541.1"
                     /translation="MADGATDRCDDWTAAVTFVPADPPRASSLACWRPDAATGLFADD
                     PGADAMLDVVVPRGDRPRRESVAVRQVTLADAIVPLLELDGAAPGVPPASVSARWWSA
                     ATVVALDLIARGHLIPAVTTGGWDAWQIDPLEPADHAVLARLVAACPPEAHAIAAPSG
                     RSEVADPAWLLRSFLDAVADAFVRTAAAPLALGSHLFAGATPSPAEPLRPWIGAAFGH
                     LTSATRLGLRVDLVQERSDDETTARFDVVLQLRSTQDPSLVVDADELADCPPMLRARL
                     GAGAGAELLVALSRAARWWPTLDRVLDHDELPGRVAVDVDEIEDLIEVAEDLEAAGVE
                     VLWPASFVADLRPRLVASAPPPVAGKEPMVGLGTLLDFRWEVLCDGVPLDDIELDALA
                     DAKRGLVWMRGRWVRTDAEVMRRIRQVPSGLDGPSGLAAALAGEVVDDDGATVEVVAA
                     GFADTLRLRLGELAGLREEAEPEGLAATLRPYQRRGVAWMADLCRAGLGGVLADDMGL
                     GKTVQVIALHLVLARSRPIAAPTLVVCPTSVLGNWAREIARFAPDVPVRKLSGPDRSL
                     AGLASDEIVLVTYGVMRRMADDLAEVGWGVVVADEAQSIKNPRARTSRAIRSIPADAR
                     IALTGTPVENRLSELWALLDWTTPGLLGSAEHFRTQFAVPIERRGVRTTSQRLSLLTK
                     PFMLRRRKTDDGVAPDLPSRTQHDVVVPLSVEQLSMYEALVRESLFQINGSEGATRVG
                     MVFRLLTALKQIANHPGQYLHEVDGPLDGRSGKLDATVELVGAAVEGEERVLVFSQYV
                     EMCHLLRRRFAEEGIDAEVLHGGLSADARDRLVDRFQAGDIPVLVISLKAGGTGLNLT
                     AATQVVHFDRWWNPAVEDQATDRAWRIGQTRPVVVHRMISEGTIDERIAEMIERKRSL
                     AESVVGGGETWIADLDDRQLAELVTLRSAAHFRSGVLL"
     gene            11663..11863
                     /locus_tag="PKY13_01085"
     CDS             11663..11863
                     /locus_tag="PKY13_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOT78542.1"
                     /translation="MATGTVKFFNTEKGYGFISREGGDDVFVHYSNIEGSGYRSLEEG
                     QAVEFDVAPGRKGEEAQNVKVI"
     gene            complement(11982..12854)
                     /locus_tag="PKY13_01090"
     CDS             complement(11982..12854)
                     /locus_tag="PKY13_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT78543.1"
                     /translation="MKIGWSLPTMIAGIDGPTVLEWCRRIEADGYSTIGFGERIAYQN
                     LELFSVLAAAAAVTERVRLISTIVVLPMHAEAWVAKQSATLDVLSGGRYTLGVGVGGR
                     NEDYRTLDSPFDHRFDRLDHQVDRIRSLWAQESPGEGLDPVGPKPVQEHLPILSGAMG
                     PKSIARSARWADGIAGFEMDPSADALGRSVAAMDAAWEAAGRDTAPYRMTSFWCALGP
                     DAESQLRSYAARYLSVFGAEFADALAASCTAAGPDAIRRAVDDARAAGFDEIQLVPTT
                     TDIAELDRMAEVLA"
     gene            complement(12860..13336)
                     /locus_tag="PKY13_01095"
     CDS             complement(12860..13336)
                     /locus_tag="PKY13_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HOT78544.1"
                     /translation="MSKRRSRIVVVALALVGAVGCGGTDYESLHRGECLPSTAKVVGE
                     REPAPPTVDCSEPHRYQVYGVTQLDGPRTYPGEEVVDQEAEHQCFLLFEPNLGYPAAQ
                     MPDDVKVVYLQPTESSWNDQADRDVECLLIYDDDTTGDAVTESNEPATETTTGGDA"
     gene            complement(13333..14151)
                     /locus_tag="PKY13_01100"
     CDS             complement(13333..14151)
                     /locus_tag="PKY13_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOT78545.1"
                     /translation="MSSRRRFVALVSAVVLVATGCSGGGDDSATTTAGSSANGIPALT
                     YEIVSRSPHDTAAFTEGFTFGDGGRLFESLGLEGNSSVREVDPTTGAVMGESRLDPAQ
                     FGEGLAVGPDGLVQLTWKDGVAHRWSIDDLTATGVFEYEGEGWGLTFDGEEFVQSDGS
                     PTLTRRDATTFEPTATVEVTRGGDPVEEINELEWVDGVIWANVWHSDEILRIDPSTGH
                     VTGVIDASALWEAPERTAEMTLNGIAHRPGDPPNRLWLTGKNWPEMFEVEVTER"
     gene            complement(14172..>15333)
                     /locus_tag="PKY13_01105"
     CDS             complement(14172..>15333)
                     /locus_tag="PKY13_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78546.1"
                     /translation="NGPLGPSDELLVHQYADTFATVSQSDLSWTEKVCAMAAARDGSM
                     QLGFGLGKYVNRDVIDAYGGVAVGNENRVVRASRQLAPEFESTAVGPLTYEIVEPMQA
                     VRFALEPTEHQPISYDVTFRAVMPARLEGRDASRSPSGARVAADLTRYHQIGVASGWV
                     DVDGVRTEVHDDEWIMTRDHSWGVRYHVGEHPRDLAPPHELDTIEGLQWRFGWSPALM
                     TTGDGEPYGIHHFFQELDAFGFQQRKLSGGIEWPDGRVEEFAALRPELTYDPANRRLI
                     GGVLHFTMADGTARPIEIEPAGDAGLHLGLGKYFGLDGHWHGAWRGPLFVEGEHVPDT
                     RPIDVQRRIHQIRDNLVRYHDPATGGTGLGNYQSTIAGAWPDLGLGQDTSFM"
     assembly_gap    15334..15433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15434..16142)
                     /locus_tag="PKY13_01110"
     CDS             complement(<15434..16142)
                     /locus_tag="PKY13_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOT78547.1"
                     /translation="MNRTDPLDLVTTGVAVGGAGIARDGDGRVVFVEGALVGERVSVE
                     IVETKKSFRRGRVLDVLEPAPGRVLPSCPEIANGCGGCDLAHASHAAQREMKAAMVAD
                     SLRRLGRLADLPDIDPGPDLTTTGFRTTIRAAVVDGRAGLRHRRSHDVVTVASCEVAH
                     PVVEGMLVNGRYGDADEVTIRVGADTGEQAVFVGGSEPDEGTVIHEEIAGRRFRISPR
                     SFFQTRADGAAALVSVVD"
     gene            16278..>17215
                     /gene="serB"
                     /locus_tag="PKY13_01115"
     CDS             16278..>17215
                     /gene="serB"
                     /locus_tag="PKY13_01115"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HOT78548.1"
                     /translation="MQANPADPTGPNDPTTETLLITVSGPDHPGVTSNLMSLLTAAGA
                     SIGDVEQVVVRGHLVLGVVATIPTTADGTNDSLRKDLLLFGYQEDLQVTFDEVDATPT
                     DRRPGLVVTVIAPTVTSTHFSAVATAIRGCGGNIDRIVRIARYPVMAYELAVHRADVE
                     SLRRDLVDLARVHRLDIALQPEGLGRRAQRLVVLDVDSTLIQDEVIELLADEAGCGDE
                     VRHITERAMAGELDFEQALRERVALLAGLTDADLLRARDRVRLTPGARTFVRTLQRLG
                     FKIAIVSGGFTYFTDHLRDELGLDHAFANELEAVDGV"
     assembly_gap    17216..17315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17316..18112)
                     /locus_tag="PKY13_01120"
     CDS             complement(<17316..18112)
                     /locus_tag="PKY13_01120"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOT78549.1"
                     /translation="MDSIEIVVDASEAELAGGIMWATGVAGIEERSQPDGRVKLIAGV
                     PEEHTDDVAAALDRHWPVVRGRMDPDQWVDSWRPYAKAARLSGGIVVQPPWIAPIAEP
                     GDLVVSLDPGRAWGHGAHPSTLLAAEDLLAAGGLDGRRVLDVGCGSGLLAIVAAIRGA
                     HTVVAVDIDPAAVEATLANAMVNGVGDLIEVSSTPVAIVDGPFDLIVANIGLSVLADL
                     ATVLTGHLVEGGAIILSGLLDDQVDAAIAAYPALTEVRRRELDGWGSV"
     gene            18213..18917
                     /gene="rpe"
                     /locus_tag="PKY13_01125"
     CDS             18213..18917
                     /gene="rpe"
                     /locus_tag="PKY13_01125"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT78550.1"
                     /translation="MSLDTPGTDREIHIAPSVLPADFARLGEECQALEKAGVDRIQWD
                     VMDGVFVPNLTFGPDVIAATRDLVAVPFEAHLMVVEPDKLMARYVEAGCQVIIVHAEA
                     CLHLHRTLGAIRELGASPAVALNPATPASSIRHVLDLVDMVLVMTVNPGFGGQAYLSS
                     MEPKVAEIRRWIVEGGYDCDLEVDGGVSPATVSGAAAAGANVLVAGSALFRDPEGLGH
                     ATAEIRRLGEAAATGA"
     gene            18914..19351
                     /locus_tag="PKY13_01130"
     CDS             18914..19351
                     /locus_tag="PKY13_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78551.1"
                     /translation="MIVRHDAARFRRRVGAVAMAVLSGSVGLTACTSSEDQFCGRLRD
                     DYQLEELVTAIEKHDQRRITERLEALRELQDIAPDEIHDDVRMVVDAVSGAVRAVTKA
                     PGANGEEVPVDLTLLRQQLAEIQEPAQHVADFADRSCGLKLNP"
     gene            19437..19886
                     /locus_tag="PKY13_01135"
     CDS             19437..19886
                     /locus_tag="PKY13_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT78552.1"
                     /translation="MTDTSRRRPSFRVLALITVCLLGVATLSTGCKPTEFSGKAQNLI
                     NSERARRGLPELAWDENAADKAQAWAQKLAASRSLSHSRLTDGIEGSWSVLGENVGFA
                     RSIDETHNGFMNSAKHREAILNGGYSSVGIGVAQNGGLVYVVEVFRG"
     gene            complement(19982..20578)
                     /locus_tag="PKY13_01140"
     CDS             complement(19982..20578)
                     /locus_tag="PKY13_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT78553.1"
                     /translation="MSSDLSIPPMLSNGFDPAGDVQNKLLKNRIVILGSEVNDQIANL
                     IAAQLLYLDGEDDSADIWLYINSPGGSVTAGLAIYDTMNFIKPNVATVCMGLAASMGQ
                     FLLCAGEKGKRFALPSARIMMHQPSGGFQGQAADIKIQAEQMVYIKQMLSERIAENTG
                     QTVEQIIKDSDRDRWFTAEQAKDYGIIDRVIVRRGETS"
     gene            20718..21167
                     /locus_tag="PKY13_01145"
     CDS             20718..21167
                     /locus_tag="PKY13_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT78554.1"
                     /translation="MKALIATDGTTAAITAAHGAVALLHPDLEIEIVRVIPEEEDPND
                     SAGGFEGPLITEEEAEAAHRADEARAQSDLESTIAALGEPATVTVVEGNDAGREICRL
                     AAERNADVLVVGASDKGWFRRLVSGSVMEYAAHHAPCPVLIVRHADD"
     gene            21360..21662
                     /locus_tag="PKY13_01150"
     CDS             21360..21662
                     /locus_tag="PKY13_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78555.1"
                     /translation="MSLAASATVAGVLVTHGGGPVAAAEQTACEYDKRIVITAIDADR
                     LTDPALDYATPAGPDGLDEVRASGWLRTETEYWRYTGPDAAGVPQLELRNPVPGCD"
     gene            complement(21686..22084)
                     /locus_tag="PKY13_01155"
     CDS             complement(21686..22084)
                     /locus_tag="PKY13_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOT78556.1"
                     /translation="MTGFPESDPGASGLHARWYEANRLAGRLTAQRCECGRWRTPARY
                     RCPGCGTDVWSFEPVAPVGRVVSWTITRRPLHFAFAEAVPYGIVVVVTDEGVRFLLQY
                     RGDPDEITIDDEVGIAVDRFGVPYADSRPD"
     gene            complement(22081..23247)
                     /locus_tag="PKY13_01160"
     CDS             complement(22081..23247)
                     /locus_tag="PKY13_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT78557.1"
                     /translation="MSAAAIVGVGETPFHRGSPLLPVELMLEASLAALDDAGLDRTDI
                     DGICPPPGYTSAEELAVNLGVTDLAFSATSSLGGASSIAAIRNAALAVRGGAASCVLV
                     VVGWNGFSWLRPREGVPVPRHGLALTSAQDVVTDVMVPQGAIGPPQFYAPVANRYRHT
                     YGIDEHDAAAFALTMRRHAANNDLAVMRDRPLTLEDYLASPMISDPFRVLDCCLETDA
                     AAAILVTSEERARDLAQPVVSVLGAGEGRPDPPDDIAGRDDLLRIGLHGAARRAFEGS
                     GVGVGDLDTVGIYDCFTYIALLQLEALGVCGPGEAGAFVRSGAAELGGALPVNTHGGL
                     LSQGHVWGMNHVVEMVRQLRGTAGASQVAGAEVGAVTGWGDFGDGTAIVLGAGR"
     gene            complement(23244..24176)
                     /locus_tag="PKY13_01165"
     CDS             complement(23244..24176)
                     /locus_tag="PKY13_01165"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT78558.1"
                     /translation="MSSLPPPPEDLPEPTGQPVPGLGHPTGAISGSWPEPPVDAPSPF
                     APPGGGAFAPPGEGFAPPGGGSPETGQGSVPPQFPPPQAPQFPPPQFPPIAGQYAAPP
                     GPGLPAPPGGWIQPEAYGEAPPPHLPPPGGPGGPGAPYGGGYQAYRPGMRAPSYAGFW
                     SRLGANFIDSLIVGVVPAILYGVGIATSPQDACTTTYDSSGGVYTSCGADLGAVGPYL
                     IAAFLAYVLLSFFVIVRPIGLTGQSIGSRATGVRVIDSATGRPIGVGRSFGRFLAALF
                     ISRWCCNLGFLWMLWDDRKQTWHDKLATAIVVDA"
     gene            24244..25032
                     /locus_tag="PKY13_01170"
     CDS             24244..25032
                     /locus_tag="PKY13_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOT78559.1"
                     /translation="MPEGHTIHRLADDLAATLAGGPVTATSPQGRFADGAALISGEPL
                     VRSDAYGKYLFCEFGGGVLLHVHLGLIGKFRPQPPDAPAGDTIRLRLENDRVAWHLTG
                     PARCDLVDPGEKAAITAHLGPDPLRRSADARRFGERLGRTTKPIGATLLDQQVIAGIG
                     NVYRAEILFLRGIHPLRPSSSLSADEIDGLWVEARRQLRRGVRLNRIVTTDPREIGRP
                     LARIEGDDRLYVYHRSHCRRCGTELRTIEVGGRPLQFCPVHQPD"
     gene            25056..26087
                     /locus_tag="PKY13_01175"
     CDS             25056..26087
                     /locus_tag="PKY13_01175"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT78560.1"
                     /translation="MTPHDLPTPALVIDLDQLDFNVDTMATVRPGAALRSHVKAHKCT
                     ELARYAAVRSGSDSFCCATVREMVGMAEAGLGVDLLLANETLDVDRLRHLAAVGAATD
                     SRITIAIDSEETLAAVVDAQSDGPLEVLVDVNVGLPRCGIAPERAGALADAARAAGIE
                     VRGVMGYEGHVVGNADRVWRAEQVELSMDQLRRAHRDVGGDVTSAGGTGTYDLHTWAG
                     EVQAGSYLLMDTAYQQLDLPFRQALSVWATVISRNDEAGFVVADAGLKAFGMDHGEPT
                     VVGPHTLFFTSDEHATIVPDPPGSITIGQRVEMWPAHIDPTMALHERVHVVRDGEVVD
                     TWPIDLRNW"
     gene            complement(26127..27068)
                     /gene="galT"
                     /locus_tag="PKY13_01180"
     CDS             complement(26127..27068)
                     /gene="galT"
                     /locus_tag="PKY13_01180"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT78561.1"
                     /translation="MSGELRLDPLTGNWVSIVGNRQARPNLPATGCPFCVGGLEAPEP
                     YDTKAFVNRWPAYAPGEPVDLAAAAATGRAAAVGAAEVILYSPVHDGSLGSLGVAQVR
                     KVVDLWAERTAALLARPEVEYVLVFESRGAEVGATIHHPHGQLYAFPFVPPLPATEAG
                     RSRDIGCVVCSSITTELDVAERVVVDDGEWAAWVPFASGYPYGVDLAPRRHVGSLADL
                     DDAGRDSLARILTDVLGRYDRLWADDPKRSPIFPYLMWIHQAPANHDGEYHVHVHLAP
                     PQRAPGVARYVAAGEAGAGTLSNPIVPEDAAATLRRA"
     gene            complement(27058..27672)
                     /gene="rdgB"
                     /locus_tag="PKY13_01185"
     CDS             complement(27058..27672)
                     /gene="rdgB"
                     /locus_tag="PKY13_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOT78562.1"
                     /translation="MALAVAAATANPKKLREIEEIFASAVGDGVELLPRPAELPDVVE
                     DGDTLAANAELKARAVADHVDGAAIADDTGLEVDALDGAPGVRSARFAGEDAVDADNV
                     KLLLERLEGVDSTHRTARFRTVICLARPGREPLFAEGVCEGRIARSPSGDGGFGYDPV
                     FVPDDGDGRTFAQMDASEKHAISHRGRAMVAAAALLREFLTDER"
     gene            complement(27686..28426)
                     /gene="rph"
                     /locus_tag="PKY13_01190"
     CDS             complement(27686..28426)
                     /gene="rph"
                     /locus_tag="PKY13_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOT78563.1"
                     /translation="MRPDGRANDELRPITIERDFTTTSHGSVLVSFGDTKVLCTATVE
                     LDVPRWMKNTGKGWVTSEYSMLPGSSPDRIGRERKGPKGRTHEIERLIGRSLRAVVDM
                     KALGERQIHLDCDVLQADGGTRTASISGAWIALHDACTRMVQAGLVSRHPVLDHCAAV
                     SVGIIEGEPRLDLPYVEDSRAETDMNVVMTGAGGLVEVQGTAEGVPFSRDELTSLLDL
                     ATGGITEIVALQREMVAEAPSARSFDKR"
     gene            28506..29801
                     /locus_tag="PKY13_01195"
     CDS             28506..29801
                     /locus_tag="PKY13_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT78564.1"
                     /translation="MGTTDVDDDATWLATLDLCDLDRFAQGFPHDTFDRLRRVAPVWW
                     HPPTPNTPDGEGFWCVTRHADVLTVVRDPETFSSESGPGRDGNGGTTLVDMPAGFVTG
                     VMMNMTDPPAHRRIRSITQAAFTPRTIARLDDWLRALAEQLVGDALDACSDEGTVDVL
                     TDVAAELPLQTIAQLMGVPQSDRHQLFEWTSTILDYGDRSLTEITDEYAAAGLGLRAY
                     GDATIADRRATVARHGAAIDGDLLGQVLAADPPLSPEEEQAFFSLLFTAGSETTRNSI
                     AGGVHAFVERPGEWRRLVDDRSLLPTAVEEILRWTSATAYNRRTATRDVELGGQRIAA
                     GQKVAHWYPSANRDESVFADAGRFDVGRSPNPHLAFGHGIHHCLGAALARRELTVMFE
                     VLADRVDEFESTGPIEWGRSNKHTSIRHLPVRLVPKGSS"
     gene            29798..31372
                     /locus_tag="PKY13_01200"
     CDS             29798..31372
                     /locus_tag="PKY13_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT78565.1"
                     /translation="MKVHLNVRDYLDRALRVYPDRTAVIDEPDQPAAPLGGGSLTYRE
                     FGEHAAALAAGLDALGVGVGERVAVVSHNSARLLVAFFGVCGWGRVLVPINFRLHPEE
                     VRYIVEQSGATVLLVDPELADAMADIECAHRFVIGDESDAVLFKFDTEPRAWEPDEDA
                     TATLNYTSGTTARPKGVDCTHRQLWMNAAVFGWQASVNDRDVYLHTLPMFHCNGWGQV
                     FSVTGMGGTHVVLRKVDGAEILRRIEGHGVTYLCAAPAVGAMVLDAAAANADGEFDPV
                     PGRDRVRMVMAGAPPPTRTIERVEVELGWEFIQIYGLTESSPLLTMSRSRSEWDDLTP
                     RERAVKLGRAGAPALGVDLQVDDQGEVIARSNHILNGYWDNPAATDEAIIDGYFHTGD
                     GGTIDDEGYLTISDRKKDVIITGGENVSSIEVEDALFNHADVAEACVIGVPSDKWGET
                     IKALVVLVPGSDLAAKSHEEVEAELIAWCRTSHAGYKCPTSIEVRDDLSRTATGKLQK
                     FRLRAPYWEGIERQVN"
     gene            31474..>32034
                     /locus_tag="PKY13_01205"
     CDS             31474..>32034
                     /locus_tag="PKY13_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78566.1"
                     /translation="MEQQRLKVHRRKTHLDGAVYTVLSPRADEGPVLSVDIVEDGATI
                     TGDTAASGLLGQLCWAMAYQHTPYTIAVVDGVAGAPVVVVNLDLGEVSEGSLTALGAL
                     LPWTTTSDGTVNLNTRSLESAVANEPAFYADQKKAGIGDIKHQSRFVRQVGDVLVLGA
                     PAIVLKSWGVQLSTPDDARPKWFLTSP"
BASE COUNT         4968 a        11530 c        10884 g         4452 t
ORIGIN      
        1 tcgtgcccat ctcgacctcg gcaactcccg gcgcgaccac cgagcccgag accgtgcaga
       61 ccacggtgcc gggcgactcg gaggtgccga gcgagcggaa ggcctcgacc ccctgagcga
      121 ggatgaacgt cacgttggca agcgtctcca cgttgtccac gagcgccggc ggcgcgtcgg
      181 tggcaccggt cggctcggac atctcgacgt gcgccgacag gccgctcccc gaggtgaggt
      241 cgaccgcgcc ggcgaccacc tcgtcgacgc ctcgccggaa cggcggggcg atgcggggga
      301 acggagggcg accgtcgagc gcctccagca tcgcggtctc ctcgccgaac aggtacgccg
      361 acggacccac gaagacgtcg acctggacgt cgtcgcacca gcgcgccgcc accagactgg
      421 cgaccgcgtc ccgcagcgcg tccgcctccg ggtggaacga ggccttcacg gcaacgatca
      481 cccggtcggc ggagacggcg tgcgccgcga tcaacgcgcc ctcgagcacc gcgtaggggt
      541 tggtccggag gatcgagcgg tccttgaacg tgcccggctc accctcggcg ccgttgacga
      601 cgaccgtggc gggagcatcg gctgcgagat tctcgatgac ggcagcccac ttccgtcccg
      661 tcgggaaccc cgcaccgccg cgaccccgca acccggcgtc atcgagcagc gccacgaccg
      721 ccgacggtgc gagctcccga gccgcagcca gacccacgcc gcccccgttg gcgaggtgat
      781 cgtcgagcgt cacgatgggc gattcgggga ggacacggtg aacgacggcc atgtcgggct
      841 cctacccgag cccgatcacc agcagacgtc ggcgcctcca ccggtaggct cgccccgatg
      901 aggatttcga tgacacatcg aggagccagg tcgacgatcg ctgcggcagt agcgatgctc
      961 gtggtgggcg ggtgcggcgg caccgacgac gccgcggatc cacccactcc gacgtccact
     1021 gccccggcgt ccaccaccac gaccacgacg acaccgtcgg tcgtgtcccg ccgtccgttc
     1081 acgaccgagc actccgagcg cacctaccaa cgaacggccg ccgacggctc cacccgcgac
     1141 ctgctcaccg aggtgtggct gcccgtgggc aacaggccgt tccccctcgt cgcgttcgcc
     1201 cacggcatgt ccggccaccc gcggaagttc cacgagctgt tcgaggcgtg ggctgccgcc
     1261 ggatacgcgg tcgttgcgcc gcgcttcccg ctcagcgccg ccgacgccgg cggctccctc
     1321 gccgacagcg tcgccgacgc accccagcag gcgctcgatc tcgagttcgt cgtcgcccgg
     1381 gtgctcgccg agtcggccga cccgaccgac ccgctccacg ggcacatcga ccccgatgcc
     1441 gttgcggtcg ccggactctc gctcggcggc ggcacggcgt tgctggcgtc ctactccggg
     1501 tgctgccccg ggctgactcc cagcgttgtg ctggccttct cacccgtccc gttcgcgacc
     1561 gagcaggccg ccgacgcggc acccctcttc ctgatgcacg gtcagaagga caccacgctt
     1621 ccctactcgg gctcccgcga cctcttcgac accgcgaaga accggcgctg gctcgtgacg
     1681 atgcccgacg ccggccacgc cgcgccctac gaggacgatc cgtcggccca ggacgacctg
     1741 gtgcggacgg tcagcctcga cgtgctcgac cagttcctgc tcggtgacga cgacggcgtc
     1801 agccgcgccg agtcgtccgt cgcagcgtcg gggctggcga cgatcgagtc ggcgggctga
     1861 ggaccggggc tcaggcctcg tcggtcacgg cgtcggccgc ggcatcggcc gcagtgtccg
     1921 ccgtgtcggc cacgaagcgc acgtcgagcg tgagctcctc cgaaccccgc acgaccgcga
     1981 ccaccaacgg gtcgccggcg tcgtgtgaag agagcgccgt gaagaggtcg tcgggccgaa
     2041 cgaggtcgac cccgccggcg gacaccagca gatcgccgat gcgaacaccg gcgacggcgg
     2101 ccgggccacc atcgacgagc ccgcgaacga ggaggccgtc tcgctgatcg aggccgaccg
     2161 ctgcccggag cttgtgcgcc acgtgggacg gagcgacctc gacgccgagg cgcagcggac
     2221 gacgcgagcg gccggcactg aggtcgtcga ccgtggaccg gagcgcatcg gacgcaacct
     2281 gcgccgccgt gaagcccttg cccaggcggt gggtgttgac ggcgacgagg cggccctcga
     2341 ggtcggtcag cggcccgccc gacgatccgc gcgccagcgg agcggtgtgc tcgagggcca
     2401 gagcgatcgg gcggccaccc gggcctcgga aggagcggga cggctggctg acggtgccga
     2461 gcgtgacgcg cacaccggca ccgtcgcggg ccgccgcgac cacgaaggcc ccgaggccgg
     2521 gagcgcgatc ggcccactcg agggccgggg cgtcgcccgt gtcgacgtcg aggaccacga
     2581 gatcatggcc cggatcggcc ccgaccacct tggcctgctc gacgcggccg tcgtcgaacg
     2641 tgatctgcac cgtgcggtca cgcaggttgt gggcgttggt gaggaccttg ccgggtgcga
     2701 cgacgacacc ggcgccgcgg tggtcgcgcc ccaccgacac ggtcgacgga gcgaccaggg
     2761 cggcggtgtg ggtgagttgt gcttcgagtt cggagatcag ggacacgatg gacatccttc
     2821 agaggtaggt gactagtcac tagcgtttga gtagtcacta ccgtagcacc aggttcgctg
     2881 ccacactggc ggggtggaac ctgccgctcc cctcgccttc gccgtcgaac ggatcggcga
     2941 ccgctggacc atcctcatcg tcgacgcgct gatgtcgggc ccccgcaagt tcggcgaact
     3001 cgaagccgac gtcaccggga tcgccccgac cgtcctcacc aagcgcctcc gtcagctcga
     3061 cgccgacggc atcgtggtcg ggcgcccgta cagcgaacgg ccgctccggt tctcctatga
     3121 gctgaccgga cccgggcgcg ccctcgccgg cgccatccag ctgctccgag actggggcgc
     3181 gtcccacacc ggtgacgagg acgccgccat gcacacagcg tgcggcagcc ccctcgtcac
     3241 ccggctgtgg tgtccatcgt gtgagaccgt ggtcggcgac gacgacgcca gcgagaacgt
     3301 cagcctctga cgaccatcgg gcgcctcagg cctcggcggg ggtcgacacc ggacccgagt
     3361 tcgccgcatc ctcgggacgc agccacacgt agacgagccc tgcgatcgca gcgcccacga
     3421 tcggcgccaa ccagaacgcc cagagctggg agagcgccgt gccaccctcg aagatcgccg
     3481 gacccgtcga acgggccggg ttgaccgagg tgttcgagat cgggatcgag atgaggtgga
     3541 tgagcgtcaa cgccagaccg atggcgagac caccgaagcc ggcggccgcc ttggtgtggg
     3601 tcgcacccag gatgatgagc aggaacatga acgtgaggaa cgcctcggcg aggagcaccc
     3661 cacccaggga gtagtagccg tgctcgaccc cgaacccgtt cgacgcgagc ttgccgtact
     3721 gcttgtcgaa cccaccgggc atggcactga cgatcgccca gatcaagccc gtcgctgcga
     3781 tcgcgcccaa cacctgcgag gcgacgtcgg ccggcaggtc cttccagtcg aaccgcttcg
     3841 cgaccgccag gccgacgctc accgccggat tgaaatggcc accggagatg tgcccgaatg
     3901 cgtaggcgcc ggtcaggacg gtgaggccga acgcgagcga caccccgacg agcccgacgg
     3961 tgtcgaggtc gccacgggtg atgatggcga acacggcggt gccgcaccca ccgaacacga
     4021 gccacaacgt tccgaagaac tcggctccac accgcttcac gagtggttga tccatgtctg
     4081 ctccttgcat tcggccagcc gcgctgaaat cgacagcccc tcccccaggg gcagatttga
     4141 ccctacgccg cccgaccgag gagtgtgcgc cttcagatct gggtgatgtt gccgatagat
     4201 gacggatgga tttcggactt cgtaacactc accggcgtcg cgccctctgc gcggtgctgg
     4261 tcctcggcct cgcacccgtg agcgccgcag ccgccgggga cacccccgca gctccgtcga
     4321 tcccctccgt cgccgtggca cacgccgacg tgctccgcag cgaacagcac ctcatcgagg
     4381 tgaccgggac cctcgacgcc gcccgcctcg agctcgactc gctcaacacc cggtccgccg
     4441 agctcgtcga tcagatcgcc gctgcccgcg ccgagctccg cgatcgcgcc gttggagcct
     4501 tcgtctcgtc ggaggaacag gacgagacgg tcctctacct cgtgggtggt caggatctgt
     4561 tcgagtcctc ggcgcggatc cgcatgatga cccattccgc cgaggtgtcg agcgcggcat
     4621 tccggcacta caacgatctg aaggccagcg tcgacccgaa ggtcgtcgag ctcggcgaac
     4681 gaatccagca gctcgaacag gacgtcgccg acgccaacga cgcggccctg gccgcccggg
     4741 cgatcgaggc cgaggcggag cgccaggcgg agcagcgggc acgagccgag cgagccgcac
     4801 gggccgccgc cgaggcggcc gcagccgcag cccgatcgac cacgatcccc acgaccgtgc
     4861 cggccgcgac cgtcgtcgcc gatcgcgccc cctccgagcc ggctccggcc gccgcggctc
     4921 cggccgcggc accgaccgcc gcaccggtgc cgctggcgac ggtgcccctc ccggccgtgc
     4981 cggccggcgg cccctccgag gctcagtggg cagcgctgcg caactgcgaa tccagcggca
     5041 actaccgagc cgtgtcgagc tcgggcaagt accgcggtgc ctaccagttc gactaccgca
     5101 cgtgggcggg cctcgggggc accggcgatc ctgctgcggc gccaccggcc gaacaggacg
     5161 cacgcgccaa gctgctctac tcccaacgtg gctggcgccc ctggcccgtc tgcggccgat
     5221 tcctccgctg accaaccgcc tcccacccga cgacccgagg tcttacgtcg gttttacaga
     5281 cgcctcctag ggtggtctcc atgtccgccg ccgaacccct cgcccacgtc ctcgtcgccg
     5341 aggacgaccg cagtgtccgc gactcgctgg cccgggtgct gcggttcgag ggctacgagg
     5401 tcgaagcggt caacgacggc gccgaggccc tcgaagccgt cacccgccgg cttcccgacg
     5461 tggtggtcat ggacgtcatg atgccgaacg tcgacggcct caccgcctgc cgcatgatgc
     5521 gcgatcgcca tccagcgctt ccgatcctca tgctcacggc ccgccacgag gtcgccgatc
     5581 gggtcgccgg cctcgacgcc ggagccgacg actatctggt caagccgttc gcactcgacg
     5641 aactgctggc ccgtcttcgg gcgctcgtcc gccgcaccag cgtcagcggg agctccgaat
     5701 cgctggtgct cgacgacctc accctcgacc ctctccgccg ccaggtcacc cgcggcgggc
     5761 gcgcgatcga cctcaccaag accgaattcg acctgctcga actgctcctg ctcaacgccg
     5821 gcatcgtgtt gtcccgcgag accatctacg accgcatctg gggcttcgac ttcgagaccg
     5881 ggtcacgttc gctcgacgtg tacatcgggt acctccgccg caagaccgag gacggcgatc
     5941 gaccccgcct cgtccacacc gttcggggcg tcggctacgt cgcccgagcg acatgagtct
     6001 tcgcctcaag atcgcggcgg cgctcgcagc gctcgcgggg atcatcgcgg cactcgccgc
     6061 cggtggcgcc tacgtgaccg cggcgcgcca actcgaccgc accatcgacg aaaccctcct
     6121 cgagcgagcg agcgccgcgg tcaacgacac gtcccgacct ggccgcggcg aaccgggcaa
     6181 ccgccaaccc ttcatgccgg gacgagcccg ctgccccgac gtcaacgtgt tgcagacctt
     6241 caccgccgcc caagtcatcg acgacggcga ggtccgccag tgcatcgtgg gcggtgcgac
     6301 catcgagccg agcgaacgtg acctggccat cgcgaacggc accgacttcc acggcgacga
     6361 cgatcatgac ggcgacgctg acgccaacta cgccttctcg agggcccgga tcggcaacga
     6421 aacggttcgt gtcatcacga tggcgttccc cgagggcggc gccatccagc tcgccaggga
     6481 aacgggcgaa gccgatcgcg tgctcgacgg gctccgcatc cgcctgctgt tgatcgcgct
     6541 cacgggcatc gtggtcgccg ccgccgtcgg gttcgccctg gcgacgcgca tcgtccgacc
     6601 catccggcga ctcaccacga ccgccgagca catcgccgcc acccgcgacc tcacgacacc
     6661 cgtgcccgcc gccggatccg acgagatcgg cagcctgtcg gccagcttca cgaccatggt
     6721 cgaatcgctg gcagagtccc aggaccaaca gcaacgactg atctccgacg cctcgcacga
     6781 gatgcgtacc cccctgacca gccttcgcac caacctggag ctgctggccc gcgccgagct
     6841 ggccgcgccc ggctcgctct ccgccgagga ccggcgcgcc gtcatcgacg acctgcaatt
     6901 cgagaccgga gagctgacgg cactcctgac cgaactggtg gaactcgcga ccgatcgtgc
     6961 cggaggcgag gagtccgttc cggtcgacct caccgttctc acgtccgggg tcgccgagcg
     7021 ggcgcggcga cggacgggcc gggtgatcga ggtcgtcggg cccgacggcg ccgacgccgg
     7081 caccggagtc gtggtcacgg tccgccagca catgatcgag cgggccgtgt ccaacctgat
     7141 cgacaacgcc gtgaagtaca gccccgacgg ggcgccggtc gacgtgatcg tcgacggtac
     7201 ccgcgtcgag gtgcgagacc acggcaacgg catcgccgac gccgacctcc cccgcatctt
     7261 cgaccgcttc taccgggcga ccgaggcccg gaccgcgccg ggatcgggcc tcgggctcgc
     7321 gatcgtcaac cagatcatcg agggccatgg cgggcgggtg ttcgccggca accacggggg
     7381 cggagccgcc gtcgggttcg agctccccac tcccccagcc tgacccgcgt ctgaccccgc
     7441 gtctgacccg gggcggcgct ccagcgccgg tgtgggtcag tcgatggtga ggtcgtcgag
     7501 gtcggctgct gcgtcgacga tgcgccagcc ggcgacatcg tcggacgcca acacgatcca
     7561 ggtgccgtcg tcggcccgac ggatctggcg cccaccgggg aggaccgcgc cggtggaccg
     7621 cgccttcacc tcgatctcga acgtcgcagc gatcagctcg agttcatcgg gaccgagacg
     7681 acggggtcgc agcagcacgt cgatgccggc tggcccacaa tgacgccagg cctcggtgag
     7741 cgcgggcgcc gattcgggcg ggcagcccgc cgccgctgcg accaccgccg acacggagtc
     7801 gggcgccgca agcgtgcccg aggcgctccg gcggacggcg tcggcgatca ggtcgagatc
     7861 gaggcccgca cccgaggccg gcgctcccaa cagcgcaccg gcccgggcag ctgcatcgcg
     7921 caccaggcga tcgagacccg cctggtcgaa gccgagttcg gcggacggcg gggcggcccc
     7981 cacccgcggc cgaccggggg cacggcggcg acgatcgatc gtcgggatct cgtcgagacg
     8041 gcgcttccac gccgtggcgg ccgacatcgt cggatcggcg ggacgacccg ggatcgccga
     8101 cacccgctga tcggcggcgg cgcgttcacg atcacgaccc gatgacaccg ggaccatgcc
     8161 ctgcaccaac gccgaccgat cgccaccacg caacatcacc aacagcagtg ggtcggcatc
     8221 gagcgcttcg gcgaggcgac acaccactgc ggccgcgtgg acacacgact cggcgaggtt
     8281 gccgcaactg cagtcgatcg agacgtcacg gacaccgggg agcagaccac agccgaccgc
     8341 ctcgaggcgg tcgtgcaacg cctcgggcag cgcgccctcg agcacggcgg cgaccagccg
     8401 gctcgaaccg gccagcgtcg cgcgaaccgt cgcccactgt tcgtcggtga accgacggac
     8461 gccgacccgg accctgcgtt cgagcgagct gcggccccgg accacaccac cgaccgaacc
     8521 cggctcgacg gtgaactcgt cgacggcccc ccgggcggcg aggccccggc cccggctcag
     8581 ctcggtgccc tcgaacgtgc gcgccgccac catccgctcg atccacgccg cgccccacga
     8641 cgtcgtgcgc atcggcgacc cggcgctcac agcaggaccc ccgagcggaa gtgggctgcc
     8701 gaccggagtg tcaccagctc ggcgagttgg cggtcgtcga gatcggcgat ccacgtctcg
     8761 ccgccaccga ccacactctc ggcgagcgaa cgcttgcgtt cgatcatctc ggcgatgcgt
     8821 tcgtcgatcg tgccctccga gatcatgcgg tggacgacca ccggtcgggt ctggccgatg
     8881 cgccacgccc ggtcggtcgc ctggtcctcg acggcggggt tccaccaccg gtcgaagtgg
     8941 acgacctggg tggcagcggt caggttgagg ccggtgccgc ccgccttgag cgagatgacg
     9001 agcacgggaa tgtcgccggc ctggaaccgg tcgacgaggc ggtcgcgggc gtcggccgac
     9061 aggccaccgt gcagcacctc cgcgtcgatg ccctcctcgg cgaaccggcg ccggagcaga
     9121 tggcacatct cgacgtactg cgagaagacc agcacacgct cctcgccctc gacggcggca
     9181 ccgaccagct ccacggtggc gtcgagcttg cccgaccggc cgtcgagcgg cccgtcgacc
     9241 tcgtgcaggt actgaccggg gtggttggcg atctgcttga gcgccgtcag cagccggaac
     9301 accatgccca cgcgggtggc gccctcggat ccgttgatct ggaacagtga ctcccgcacc
     9361 agcgcctcgt acatcgacag ttgctcgacg ctgagcggca ccaccacatc gtgctgggta
     9421 cgactcggca ggtcgggggc gacgccgtcg tcggtcttgc ggcgccgcaa catgaacggc
     9481 ttggtgagga gggacagtcg ctgggacgtc gtgcgaacgc cccgacgctc gatcggcacc
     9541 gcgaactggg tgcggaagtg ctcggcgctg ccgaggaggc ccggcgtcgt ccagtcgagc
     9601 agggcccaga gctcgctcag gcggttctcg accggggtac cggtgagggc gatccgggcg
     9661 tcggcgggga tgctacggat cgcccgggac gtacgcgccc gggggttctt gatcgactgc
     9721 gcctcgtcgg ccacgaccac accccacccg acctcggcca ggtcgtcggc catgcgccgc
     9781 atcacgccgt aggtgacgag cacgatctcg tcggacgcca ggccggcgag gcttcgatcg
     9841 ggacccgaca gcttgcggac cgggacgtcg ggagcgaacc gggcgatctc ccgcgcccag
     9901 ttgccgagca ccgacgtggg acacacgacc agggtcggcg cagcgatcgg gcgggatcgc
     9961 gccaggacca ggtggagggc gatgacctgc acggtcttgc cgagccccat gtcgtcggcc
    10021 aggactcccc cgagaccggc ccggcacagg tcggccatcc acgccacgcc acgtcgctga
    10081 tacgggcgga gcgtcgcggc cagcccctcg ggctcggcct cctcgcggag acccgccagc
    10141 tcgccgagcc gcaggcgcaa ggtgtcggcg aagccggcgg cgacgacctc gacggtggcc
    10201 ccgtcgtcgt cgaccacctc gccggccagc gctgccgcca ggcccgacgg gccgtcgagg
    10261 ccactcggga cctggcggat gcggcgcatc acctcggcgt cggtgcgcac ccaacggccc
    10321 cgcatccaga cgaggccgcg cttggcgtcg gcgagcgcgt cgagctcgat gtcgtcgagt
    10381 ggcaccccgt cgcacaggac ctcccaccgg aagtcgagca gcgtgcccaa ccccaccatc
    10441 ggctccttgc cggcgacggg aggaggcgcc gacgcgacga ggcggggtcg gagatcggcg
    10501 acgaacgacg ccggccacag cacctcgaca ccggcggcct cgaggtcttc cgcgacctcg
    10561 atgagatctt cgatctcgtc cacgtcgacc gcgacgcgac cggggagttc gtcgtggtcg
    10621 agcacccggt cgagcgtcgg ccaccaccga gccgctcggc tgagggcgac caacaactcg
    10681 gctccggcgc cggcaccgag acgcgcccgg agcatcggcg ggcagtcggc caactcgtcg
    10741 gcgtcgacga ccaacgacgg atcctgcgtg ctgcgcagct ggaggacgac gtcgaaccgg
    10801 gcggtggtct cgtcgtcact gcgttcctgt acgaggtcga cgcggagccc gaggcgggtg
    10861 gcgctggtga ggtggccgaa ggctgcaccg atccacggtc ggagcggctc ggccggcgac
    10921 ggggttgcgc cggcgaacag gtgcgaaccc agcgcgagcg gcgccgcggc cgtacggacg
    10981 aacgcgtcgg ccacggcgtc gaggaacgat cgcaacagcc acgccgggtc ggccacctcg
    11041 gaccggcccg acggtgctgc gatcgcatgc gcctcgggtg ggcatgccgc cacgagccga
    11101 gccagcacgg cgtggtcggc cggttcgagg gggtcgatct gccaggcgtc ccacccgccc
    11161 gtcgtcacgg ccgggatgag atgaccgcgg gcgatcaggt cgagcgcaac gaccgtcgcc
    11221 gccgaccacc atcgcgccga gaccgacgcc ggcggcaccc cgggcgcagc accgtcgagc
    11281 tcgagcagcg gcacgatggc gtcggccagg gtgacctgcc ggacggccac cgactcacga
    11341 cgcggccggt cgccgcgagg caccacgacg tcgagcatcg cgtcggcacc cggatcgtcg
    11401 gcgaacaggc ccgtggccgc gtcgggccgc cagcacgcga gactcgacgc ccgcggcgga
    11461 tcggccggca cgaacgtgac cgccgccgtc cagtcgtcac accgatcggt ggccccgtct
    11521 gccatgacgg acaccatagc gcgcgacacg aacaggtgtt cgaggtccga actttcccgc
    11581 ccaccgggcg ggaaaggctg cctacactga cctcattcga gcgggccaca agggctcgtt
    11641 caatgcgaga gaagagaatc cgttggcaac agggaccgtg aagttcttca acactgagaa
    11701 gggctacggc ttcatctcac gtgagggagg cgatgatgtg ttcgtccact actccaacat
    11761 cgaaggaagc ggctaccgct cactcgagga aggccaggcc gtcgagttcg acgtcgctcc
    11821 gggccgcaag ggcgaggaag cccagaacgt gaaggtcatc tgaccaccga cgagacgctt
    11881 ctcaaccgag agatcgagat gaacgaccga ggcctggcct cggtcgttcg tcgtggtggg
    11941 cccgggtccg gctgacccgg cgtccatcgc actcggccgc gtcaggcaag cacctcggcc
    12001 atccggtcga gttcggcgat gtcggtcgtc gtcggcacga gttggatctc gtcgaaaccg
    12061 gcggcgcggg cgtcgtcgac agcgcggcgg atcgcgtcgg gccccgccgc ggtgcacgac
    12121 gccgccagcg cgtcggcgaa ctcggcgccg aacaccgaca ggtagcgcgc cgcgtacgag
    12181 cgcaactggc tctcggcatc ggggccgagc gcgcaccaga agctcgtcat gcggtagggg
    12241 gcggtgtcac ggcccgccgc ctcccacgcc gcgtccatcg cagcaacact tcgcccgagg
    12301 gcgtcggccg acgggtccat ctcgaacccg gcgatcccgt ccgcccagcg cgccgatcgg
    12361 gcgatcgact tgggacccat cgcaccgctc aggatcggca ggtgctcctg gaccggcttg
    12421 ggaccgacgg ggtcgaggcc ctcgcccggc gactcctggg cccacaacga acgaatgcgg
    12481 tcgacctggt ggtcgagccg atcgaaccgg tggtcgaacg gcgagtccag cgttcggtag
    12541 tcctcgttgc gtccgccgac cccgacgccg agcgtgtacc ggccgccgga gagcacatcg
    12601 agggtcgccg attgcttggc cacccacgcc tcggcgtgca tgggcagcac cacgatggtg
    12661 gagatcaggc gcacccgctc ggtcacggca gcggccgccg ccagcaccga gaacagctcg
    12721 aggttctggt aggcgatgcg ctcgccgaac ccgatcgtcg aatagccgtc cgcctcgatg
    12781 cgccgacacc actcgagcac ggtggggccg tcgatcccgg cgatcatggt ggggagcgac
    12841 caaccgatct tcatgtcggt cacgcgtctc cccccgtcgt cgtctcggtc gccggctcgt
    12901 tgctctcggt cactgcgtcc cccgtggtgt cgtcgtcgta gatcagcagg cactccacgt
    12961 cgcggtcggc ctggtcgttc cagctggatt cggtgggctg aaggtagacc accttcacgt
    13021 cgtcgggcat ctgcgccgcg gggtagccga ggttcggctc gaacagcagg aaacactgat
    13081 gctccgcctc ctggtcgacc acctcctcgc ccggataggt gcggggtccg tcgagttggg
    13141 tcacgccgta gacctggtag cggtgcggtt cggagcaatc gaccgtgggc ggggcgggct
    13201 cacgctcccc caccaccttc gccgtgctcg gcaggcactc gccgcggtgc aacgactcgt
    13261 agtcggtgcc accgcacccg accgcgccga cgagtgccaa cgctacgacc acgatcctcg
    13321 acctgcgttt gctcatcgct ccgtcacctc gacctcgaac atctcgggcc agttctttcc
    13381 ggtcagccac agtcggttcg gcgggtcgcc gggccgatgg gcgatgccgt tgagggtcat
    13441 ctcggcggtc cgctccgggg cctcccacag cgccgacgcg tcgatcacgc cggtcacgtg
    13501 accggtcgac gggtcgatgc ggaggatctc gtcggagtgc cacacgttcg cccagatcac
    13561 accgtccacc cactcgagtt cgttgatctc ctcgaccgga tcgccgccgc gggtgacctc
    13621 gacggtggcg gtcggttcga acgtcgtcgc gtcgcggcgg gtgagcgtcg gcgagccgtc
    13681 gctctggacg aactcctcgc cgtcgaacgt gaggccccag ccctcgccct cgtactcgaa
    13741 gacgcccgtt gcggtgaggt cgtcgatcga ccagcggtgg gcgacgccgt ccttccacgt
    13801 cagttgcacg agcccgtcgg gacccaccgc gaggccttcg ccgaactgcg cggggtcgag
    13861 gcgcgactcc cccatgaccg ctcccgtcgt gggatcgacc tcgcggaccg aggagttgcc
    13921 ctccaacccg aggctctcga acaggcgccc gccgtcgccg aaggtgaagc cctcggtgaa
    13981 cgctgcggtg tcgtggggag accggctcac gatctcgtag gtcagcgccg ggatgccgtt
    14041 ggcgctcgac cctgccgtcg tcgttgccga gtcgtcaccg cccccactgc aacctgtggc
    14101 caccagcacc acagcgctca cgagcgcgac gaatcggcgg cgtgacgaca tgggcggaac
    14161 cctacgccgc ctcacatgaa gctggtgtcc tgccccaggc ccaggtcggg ccaggcgccg
    14221 gcgatcgtgg actggtagtt gccgagcccg gtcccaccgg tcgccgggtc gtggtagcgg
    14281 acgaggttgt cgcggatctg atggatccgg cgctgcacgt cgatcggccg ggtgtcgggc
    14341 acgtgttctc cctcgacgaa gagcgggccc cgccacgcac cgtgccagtg gccgtcgaga
    14401 ccgaagtact tgccgagacc gaggtgcaac ccggcgtcgc ccgccggctc gatctcgatc
    14461 ggccgcgccg tcccgtcggc catcgtgaag tggaggaccc caccgatgag acgtcggttg
    14521 gcggggtcgt aggtgagctc gggacggagg gcggcgaact cctcgacacg accgtcgggc
    14581 cactcgatgc cgccggacag cttgcgctgt tggaagccga acgcgtcgag ctcctggaag
    14641 aagtggtgga tgccgtacgg ctcgccgtcg cccgtcgtca tcaacgccgg cgaccagccg
    14701 aaccgccact gcagtccttc gatcgtgtcc aactcatggg gcggcgcgag atcgcgaggg
    14761 tgctcaccca cgtggtaccg gactccccag ctgtggtcgc gggtcatgat ccactcgtcg
    14821 tcgtgcactt cggtacgcac gccgtccacg tcgacccaac ccgacgcgac gccgatctgg
    14881 tggtagcgcg tgaggtcggc ggcgacccgc gctcccgacg gactacgtga tgcgtcacgc
    14941 ccttcgagcc gcgccggcat cacggcgcgg aacgtcacgt cgtaggagat cggctggtgc
    15001 tcggtcggtt cgagtgcgaa ccgcacggcc tgcatcggct cgacgatctc gtaggtcagg
    15061 ggcccgaccg cggtcgactc gaactcgggc gccagctgcc ggctggcccg cacgacccgg
    15121 ttctcgttgc cgacggcgac accgccgtag gcatcgatga cgtcgcggtt cacgtacttg
    15181 cccaggccga agccgagttg catgctgccg tcacgggcgg ccgccatggc gcacaccttc
    15241 tccgtccacg agaggtcgct ctggctgacc gtcgcgaagg tgtcggcgta ctggtggacg
    15301 agcagctcgt ccgaggggcc gagcgggccg ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnncgtcgac gacggacacg agggcggcgg cgccgtcggc gcgcgtctgg
    15481 aagaacgaac gaggcgagat ccggaagcgg cgaccggcga tctcctcgtg gatgaccgtg
    15541 ccctcgtccg gctcactgcc gccgacgaac acggcctgct cgccggtgtc ggcgcccacc
    15601 cggatcgtca cctcgtcggc gtcgccgtag cggccgttca ccaacatccc ctcgacgacc
    15661 ggatgcgcga cctcgcacga ggccacagtg acgacatcgt gggaccggcg gtggcgcagg
    15721 ccggcgcgtc catcgacgac tgcggcgcgg atcgtggtgc ggaatccggt cgtggtcagg
    15781 tcggggcccg ggtcgatgtc gggaaggtcg gcgaggcgac cgagacgtcg tagcgagtcg
    15841 gcgaccatcg ccgccttcat ctcgcgttgc gccgcgtgcg atgcgtgggc gaggtcgcac
    15901 ccaccgcagc cgttggcgat ctcgggacag ctcgggagca cccgaccggg cgccggttcg
    15961 agcacgtcga gcacccgacc acggcggaac gacttcttcg tctcgacgat ctcgacgctg
    16021 acccgttcac ccacgagggc accctcgacg aacacgacgc gaccgtcacc gtcgcgggcg
    16081 atacccgctc ccccgacggc cacaccggtc gtcacgaggt cgagcgggtc ggtccggttc
    16141 acgctgccga ccctacgctg cgggcacgct cacgttcgtc gccgagcgag acacccatca
    16201 accagcgacg cacattcggt ggtgatcacc ggcccccgat gtcaggtccg gtggggtgtc
    16261 cccggtagcg ttggggcgtg caggcgaacc cggccgaccc caccggtccg aacgacccga
    16321 cgactgagac cctcctcatc accgtctccg gtcccgacca ccccggcgtc acgtccaacc
    16381 tcatgtcgct gttgacggcc gcgggcgcgt cgatcggcga cgtcgaacag gtggtggtcc
    16441 gcggtcacct cgtcctcggg gtcgttgcga ccatcccgac gaccgccgac ggcaccaacg
    16501 actcgctccg caaggacctg ctgctgttcg gctaccagga ggatctccag gtcacgttcg
    16561 acgaggtcga cgccaccccc accgatcgcc ggcccggcct cgtcgtcacg gtgatcgctc
    16621 cgacggtcac gtccacgcac ttcagcgccg tggcaaccgc gatccgcggc tgcggtggca
    16681 acatcgaccg catcgtgcgc atcgcccgct acccggtgat ggcgtacgag ctggcggtgc
    16741 accgggccga cgtcgagtcg ctccgccgcg acctggtcga cctcgcccgg gtccaccggc
    16801 tcgacatcgc cctgcaaccc gagggtctcg ggcgccgcgc ccagcgcctc gtggtgctcg
    16861 acgtcgattc gaccctgatc caggacgagg tcatcgagct gctcgccgac gaggcgggct
    16921 gtggggacga ggtccgccac atcaccgaac gggcgatggc cggcgagctc gacttcgagc
    16981 aggccctacg cgaacgggtg gcgctgctcg ccggtctgac cgacgccgac ctgctccggg
    17041 cccgcgaccg cgttcgcctc acgcccggcg ctcgcacgtt cgtccggacg ctgcaacgcc
    17101 tcggcttcaa gatcgcgatc gtgtcgggcg gcttcacgta cttcacggat cacctgcgcg
    17161 acgaactcgg gctcgaccac gcgttcgcca acgagctgga ggccgtcgac ggcgtnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccga gccccacccg tcgagctcgc
    17341 ggcgccgcac ttcggtgagg gcgggatagg cggcgattgc ggcatcgacc tgatcgtcga
    17401 ggagcccact caggatgatc gctccgccct cgaccaggtg tccggtgagg acggtggcga
    17461 ggtcggccag cacggacagg ccgatgttgg ccacgatgag gtcgaaggga ccgtcgacga
    17521 tggcgacggg cgtggacgac acctcgatga ggtcccccac gccgttgacc atcgcgttgg
    17581 cgagcgtcgc ctcgacggcg gccgggtcga tgtcgacggc gacgacggtg tgggctcctc
    17641 tgatcgccgc gacgatggcg aggagaccgc tgccgcaccc gacgtcgagg acgcgccgac
    17701 cgtcgagccc tccggctgcg aggaggtcct cggcggcgag cagcgtggac ggatgcgccc
    17761 cgtgacccca ggctcgaccg gggtcgaggg acaccacgag gtcgcccggt tcggcgatgg
    17821 gagcgatcca cggcggctgc acgacgatgc ccccgctcag gcgcgccgcc ttggcgtacg
    17881 gccgccaact gtcgacccac tggtcggggt ccatccgccc gcgaacgacg ggccagtgcc
    17941 ggtcgagcgc tgcggcgacg tcgtcggtgt gttcctcggg gacaccggcg atcagcttca
    18001 cccgcccatc gggctgggag cgttcctcga tccccgcgac gccggtcgcc cacatgatcc
    18061 ccccggccag ctcggcctcg gaggcgtcca ccacgatctc gatcgaatcc acgtccagca
    18121 gggtacggcg gcgagccgcc agtgcggtca gcggcgagcc gccagtgcgg tcagcggcga
    18181 gccgccggtg cggtcagcgc tagtttgggg cgatgtccct cgacacgccc ggtaccgacc
    18241 gagagatcca catcgccccg tccgtgctcc ccgccgactt cgcccggttg ggcgaggagt
    18301 gccaggccct cgagaaggcc ggcgtcgacc gcatccagtg ggacgtgatg gacggcgtct
    18361 tcgtccccaa cctgaccttc gggcccgacg tcatcgctgc cacccgagac ctcgtcgccg
    18421 tgccgttcga ggcgcacctc atggtcgtcg agcccgacaa gctgatggct cgctacgtcg
    18481 aagccggctg tcaggtgatc atcgtccacg ccgaggcatg cctccacctg caccgcacgc
    18541 tcggggcgat ccgcgagctg ggcgcgtcgc ctgccgtcgc gctcaaccct gcaacaccag
    18601 cgtcatcgat ccgtcacgtt ctcgacctcg tcgacatggt gctcgtcatg acggtgaacc
    18661 ccggtttcgg tgggcaggcc tacctgtcgt cgatggaacc gaaggtcgcc gagatccggc
    18721 gttggatcgt cgagggcggc tacgactgcg acctcgaggt cgacggcggt gtgtcaccgg
    18781 cgacggtgtc gggtgccgcc gcagcagggg ccaacgtgtt ggtcgccggc agtgcgctct
    18841 tccgtgatcc cgaggggctg ggccatgcca cggccgagat ccggcgactc ggcgaggcgg
    18901 cggcgaccgg ggcgtgatcg tgcgccacga cgccgcccgt ttccgccgac gggtcggggc
    18961 ggtcgcgatg gccgtgttgt cggggtccgt cggcctcacc gcgtgcacga gcagcgaaga
    19021 ccagttctgc ggtcgcctgc gcgacgacta ccagctcgag gagttggtca ccgccatcga
    19081 gaagcacgat cagcggcgga tcaccgagcg cctcgaagcg ctccgtgaac tccaggacat
    19141 cgctcccgac gagatccacg acgacgtccg gatggtcgtc gacgcggtca gcggcgcggt
    19201 gcgcgccgtg accaaggcac ccggtgccaa cggcgaggag gtccccgtcg acctcacgtt
    19261 gctccgccag cagttggccg agattcagga acccgctcaa cacgtcgcgg attttgccga
    19321 caggagttgt gggttgaagc tcaatccgta atcctttcgt catcagaatg caacattggg
    19381 cctcggagtc gtgttaggtt ctcggcctac atgaggggtt ctgctggagg agcaccatga
    19441 ctgacacgtc acgccgtcgt ccgagtttcc gcgtacttgc gctcatcacg gtctgtctcc
    19501 tcggggtcgc gaccctgagc accggctgca agccgaccga gttcagcggc aaggcccaga
    19561 acctcatcaa cagcgagcgg gccaggcgtg gcctccccga actggcctgg gacgagaacg
    19621 ccgccgacaa ggcgcaggcg tgggcgcaga agctggcggc gagccgctcg ctctcccaca
    19681 gccggctcac cgacggcatc gagggcagct ggtccgtgct cggcgagaac gtcggcttcg
    19741 cccgcagcat cgacgagacc cacaacgggt tcatgaactc ggcgaagcac cgcgaggcga
    19801 tcctcaacgg tggctacagc tccgtcggga tcggcgtcgc ccagaacggc gggctcgtgt
    19861 acgtcgtcga agtgttccga ggctgacccg acccgaccga ggtcgccccg caatcagaac
    19921 ggcccgctca ctcgatcgag tgagcgggcc gtttctgttg tcgatcgatg tcgacgtgcg
    19981 atcagctcgt ctcgccgcgg cggacgatga cccggtcgat gatgccgtag tccttggcct
    20041 gctcggcggt gaaccaacgg tcacggtcgg agtccttgat gatctgctcg acggtctgac
    20101 cggtgttctc ggcgatgcgc tcggacagca tctgcttgat gtacaccatc tgctcggcct
    20161 ggatcttgat gtcggccgcc tggccctgaa agccgccgga cggctggtgc atcatgatgc
    20221 gagcgctcgg cagggcgaag cgcttgccct tctcaccggc gcacaacagg aactggccca
    20281 tcgacgcagc aaggcccatg cacacggtgg ccacgttggg cttgatgaag ttcatcgtgt
    20341 cgtagatggc caggccggcc gtcaccgagc cgccgggcga gttgatgtac agccagatgt
    20401 cggccgagtc gtcctcgccg tcgaggtaga gcagctgggc cgcgatgagg ttcgcgatct
    20461 ggtcgttgac ctcggaaccg aggatgacga tgcggttctt gagcagcttg ttctggacgt
    20521 caccggcggg gtcgaagccg ttgctcaaca tcgggggaat cgagagatct gaagacattc
    20581 cggtcaggtt agctgcgctg accgccgacg aggttccgcc ggcgttcgac ggcgcgcccc
    20641 gacggagcag gcacaactgt gctgaagtac ggaccaggtc atcgagccga cttcgcccga
    20701 tccacagagg agcacccatg aaggcactca tcgccaccga cgggacgacc gcagcgatca
    20761 ccgccgcgca cggagcggtc gcgctgctcc atcccgatct cgagatcgag atcgtacgcg
    20821 tcatccccga ggaggaggac cccaacgaca gcgccggcgg attcgagggg ccgctcatca
    20881 ccgaggagga ggccgaggcc gcccaccgcg ccgatgaggc acgggcccag tccgatctgg
    20941 aatcgacgat tgccgcactc ggggaacccg ccaccgtcac cgtggtcgaa ggcaacgacg
    21001 ccggccgcga gatctgccgc ctggccgccg aacgcaacgc cgacgtgctg gtcgtcggcg
    21061 caagcgacaa ggggtggttt cgccgcctcg tctcgggatc ggtgatggag tacgccgcgc
    21121 accatgcgcc gtgcccggtg ctgatcgtgc gtcacgccga cgactgatcg aggccctcga
    21181 ccgcgtccgc cggaagttga gggaccgggc gactcttttc tcaagacgat tcctgcagcc
    21241 gtcgaaccaa gtgaggtcaa tcgacgaatc aggaagttgg ggacatgcag caggctggtt
    21301 ggtggacgaa ccgcgtccga actcggggac agggtggctt ctcggccatg ggtgtcctca
    21361 tgtcgctggc cgcatcggcg accgttgcgg gtgtgctcgt gacccacggc gggggcccgg
    21421 tcgcggcggc cgagcagacg gcgtgcgagt acgacaagcg gatcgtcatc acggcgatcg
    21481 acgccgaccg cctgaccgac cccgccctcg actacgcaac gccggccggg cccgacggac
    21541 tcgacgaggt tcgggccagc gggtggctcc gcaccgagac cgagtactgg cgctacacgg
    21601 gccccgacgc cgccggggtg ccacaactcg agctgcggaa cccggtgccc ggctgcgact
    21661 gaccagcgtc ggccggtcag tcgggtcagt cgggtcggga atcggcgtac ggcaccccga
    21721 agcgatcgac cgcaatgcca acctcgtcat cgatcgtgat ctcgtcgggg tcaccccggt
    21781 actgcaacag gaagcgaacg ccttcgtcgg tgacgacgac cacgatgccg tagggcacgg
    21841 cctcggcgaa cgcgaagtgc agcggacggc gggtgatcgt ccagctcacc acccgtccca
    21901 cgggagcgac cggctcgaac gaccacacgt cggtgccgca ccctggacac cggtagcggg
    21961 caggggtacg ccaccggccg cactcgcagc gctgggccgt caggcggccg gcgagccggt
    22021 tggcctcgta ccaacgggcg tggaggcccg acgcgcccgg gtcggactcc gggaaccccg
    22081 tcatcgaccc gcccccaata cgatcgcggt gccatcgccg aagtcgcccc acccggtgac
    22141 cgcgccgacc tcggcgccgg cgacctgact ggcaccggcg gtgccccgga gctgacgcac
    22201 catctcgacg acgtggttca tcccccacac atgcccctgt gacagcagtc caccgtgggt
    22261 gttcaccggc aacgccccac cgagctcggc ggcgccgctc cgcacgaacg cacccgcctc
    22321 acccggtccg cacaccccga gcgcctccag ctgcagcagg gcgatgtagg tgaagcagtc
    22381 gtagatgccg acggtgtcga gatcgccgac accgacgccg gatccctcga aggcgcgtcg
    22441 ggcggcgccg tggagcccga tgcgcaggag gtcgtcgcgg ccggcgatgt cgtcgggtgg
    22501 atcggggcgc ccctcgccgg cgcccagcac cgagaccacc ggctgggcga ggtcgcgggc
    22561 ccgttcctcc gaggtcacca ggatcgccgc ggcagcgtcg gtctcgaggc agcagtcgag
    22621 cacccgaaac gggtcggaga tcatcggcga tgccaggtag tcctcgagcg tgagggggcg
    22681 atcgcgcatg acggcgaggt cgttgttcgc ggcatggcga cgcatcgtca gtgcgaacgc
    22741 cgcggcatcg tgctcgtcga tcccgtacgt gtgtcggtac cggttggcga cgggcgcgta
    22801 gaactggggt ggcccgatgg cgccctgggg caccatcacg tcggtgacga cgtcctgagc
    22861 cgacgtcagc gcgagaccgt gccggggcac cgggacgccc tcgcgggggc gcaaccacga
    22921 gaacccgttc cacccgacga cgaccagcac gcacgacgct gcgccaccgc ggaccgcaag
    22981 cgctgcgttg cggatggcgg cgatgctcga cgctccgccg agcgacgagg tcgcggagaa
    23041 ggccaggtcg gtcaccccga ggttgacggc gagctcctcg gcgctggtgt agcccggcgg
    23101 cgggcagatg ccgtcgatgt cggtccggtc gaggccggcg tcgtcgaggg cggcaaggga
    23161 tgcctcgagc atgagctcga cggggaggag cggcgagccc cggtggaatg gcgtctcgcc
    23221 gaccccgacg atcgctgcgg cgctcacgcg tcgaccacga tcgccgtggc gagcttgtcg
    23281 tgccacgtct gctttcggtc gtcccacagc atccacagga accccaggtt gcagcaccag
    23341 cgggagatga acagcgctgc caggaaccgt ccgaacgacc ggccgacccc gatcggtcgg
    23401 cccgtggcgg agtcgatgac ccggaccccg gtggcccggc tcccgatcga ctgaccggtg
    23461 agaccgatgg gccggacgat gacgaagaac gacagcagga cgtatgcgag gaacgctgcg
    23521 atgaggtacg gaccgacagc cccgagatcg gcgccgcacg acgtgtacac cccgccgctc
    23581 gagtcgtagg tcgtggtgca cgcgtcctgc ggggacgtcg cgatgccgac gccgtagagg
    23641 atcgccggaa ccacacccac gatcaggctg tcgatgaagt tcgccccgag acgggaccag
    23701 aacccggcgt agctcggggc gcgcatgccg ggccgatagg cctggtagcc cccaccgtag
    23761 ggagcgcctg ggccgccggg tccgccgggt ggtgggaggt gcggaggtgg agcctcgccg
    23821 taggcctcgg gttggatcca acctcccggt ggagcgggca gcccgggacc gggaggtgcg
    23881 gcgtactggc cggcgatcgg tgggaactgg ggcggcggga actgcggtgc ctgcgggggc
    23941 gggaactgcg gcgggaccga cccttgcccg gtctcgggcg accctccgcc gggcggtgcg
    24001 aacccctcgc cgggcggagc gaacgcgcca ccgccgggcg gagcgaacgg cgacggcgcg
    24061 tcgaccggcg gctcgggcca cgaccccgaa atcgcacccg tgggatggcc gagccccggt
    24121 accggctggc ccgtcggctc gggcaggtcc tccggcggtg gcggcagcga gctcatcggg
    24181 cactccttgg gggcgccccg gcgacgacct cgccggggac cgacaaccgt aatactggcc
    24241 gccatgcccg agggacatac gattcaccgc ctcgcagacg atctcgctgc cacgctcgcc
    24301 ggcgggcccg tcaccgcgac ctcgccgcaa ggccggttcg ccgacggggc ggcactgatc
    24361 tccggcgaac cgctcgtgcg cagcgacgcc tacggcaagt acctgttctg cgagttcggc
    24421 ggcggtgtgc tcctccacgt ccacctcggt ctcatcggca agttccgtcc ccaaccgccc
    24481 gacgccccgg ccggcgacac catccggctc cgcctcgaga acgaccgggt cgcctggcac
    24541 ctcaccgggc cggcccgctg cgacctcgtc gatcccggcg agaaggcggc gatcaccgcc
    24601 cacctcggcc ccgaccccct ccggcggagc gctgacgcgc gccgattcgg ggagcgtctg
    24661 ggccggacga ccaaaccgat cggagcgacc ctcctcgacc agcaggtgat cgccggcatc
    24721 ggcaacgtgt accgcgccga gatcctcttc ctccgtggca tccatccgct ccgcccgtcg
    24781 tcgtcgctgt ccgccgacga gatcgacgga ttgtgggtcg aggcccgacg gcagctgcgg
    24841 cggggcgttc gcctcaaccg gatcgtcacc accgatcccc gcgagatcgg ccgccccctc
    24901 gcccgcatcg agggcgacga tcgcctgtac gtgtaccacc ggtcccactg ccgtcgctgc
    24961 gggaccgagc tgcgcacgat cgaagtcggc ggacggcccc tccagttctg ccccgtccac
    25021 caaccggact gacggcgcgg cgatagcgtg cggcgatgac gccccacgac ctgcccaccc
    25081 cggcgcttgt catcgacctc gaccagctcg acttcaacgt cgacaccatg gcgacggttc
    25141 gccccggagc cgccctgcgg tcacacgtca aggcccacaa gtgcaccgag ttggcgcgct
    25201 acgccgcggt ccgctccgga tccgactcgt tctgctgcgc cacggtgcgg gagatggtcg
    25261 gcatggccga ggccggcctc ggcgtcgacc tgctgctcgc caacgagacc ctcgacgtcg
    25321 atcggctccg gcacctcgcc gccgtcgggg cggcaaccga ctcgcggatc acgatcgcga
    25381 tcgactccga ggagacactc gccgccgtcg tcgacgcgca gagcgacggc cccctcgagg
    25441 tgctggtcga cgtcaacgtc gggctccccc gctgtggcat cgcacccgaa cgggccggag
    25501 cgctcgccga cgccgcccgc gccgcgggca tcgaggtgcg cggcgtgatg gggtacgagg
    25561 gccacgtcgt cggcaacgcc gaccgggtct ggcgcgccga gcaggtcgag ctgtcgatgg
    25621 accagttgcg tcgggcccac cgcgacgtcg gcggcgacgt cacctcggcc ggcggcaccg
    25681 gcacctacga cctccacacg tgggccggtg aggtgcaggc gggttcgtac ctgctcatgg
    25741 acaccgctta ccagcagctc gacctgccct tccgccaggc actctcggtg tgggcgacgg
    25801 tcatctctcg caacgacgag gccgggttcg tcgtcgccga cgccggcctc aaggcgttcg
    25861 gcatggacca cggcgaaccg accgtcgtcg gcccccacac cctgttcttc acctccgacg
    25921 aacacgcgac gatcgtgccc gacccgcccg gcagcatcac gatcggacag cgggtcgaga
    25981 tgtggcccgc gcacatcgac cccacgatgg cgttgcacga gcgggtccac gtcgttcgcg
    26041 acggagaggt cgtcgacacc tggccgatcg atctgcgcaa ctggtgacgc caccgtgcgg
    26101 cggcgccggc gagaacaggc gtctggtcag gcccgccgca gcgtggccgc agcgtcctcg
    26161 ggaacgatgg gattcgacaa cgtgccggcg ccggcttcgc ccgcagcgac gtagcgagcc
    26221 acgccgggag cccgctgcgg tggcgccagg tgcacgtgca cgtggtactc gccgtcgtga
    26281 ttcgcgggag cctgatggat ccacatcagg tacgggaaga tggggctgcg cttcgggtcg
    26341 tccgcccaga gtcggtcgta acggcccagc acatcggtga ggatccgggc cagcgagtcg
    26401 cgtccggcgt cgtcgagatc ggccagtgaa cccacgtgcc gacgcggcgc caggtcgacg
    26461 ccgtacggat agcccgacgc gaacggcacc caggccgccc actcgccgtc gtcgacaacc
    26521 acacgctcag cgacgtcgag ctccgtcgtg atcgacgagc acaccacaca accgatgtcg
    26581 cgggagcgcc cggcctcggt cgccggcagc ggcggcacga aggggaacgc gtagagctgc
    26641 ccgtggggat gatggatcgt cgcccccacc tcggcgccgc ggctctcgaa cacgaggacg
    26701 tactcgactt cgggacgcgc caggagtgcc gcggtgcgct cggcccacag gtcgacgacc
    26761 ttgcggacct gcgccacgcc cagcgacccg agcgagccgt cgtgcaccgg ggagtagagg
    26821 atcacctccg cagcgccgac cgccgcggcg cgtcccgtcg ccgcagccgc cgcgagatcg
    26881 accggctcgc cgggggcgta ggcgggccac cggttcacga acgccttggt gtcgtagggc
    26941 tccggcgcct cgaggccacc gacacagaac gggcagccgg tcgccggcag gttcgggcgc
    27001 gcctggcggt tcccgacgat gctgacccag ttgcccgtca gcggatcgag ccgcagctca
    27061 ccgctcatcg gtgaggaact cccggagcag cgcggccgca gcgaccatcg cccggccccg
    27121 gtggctgatc gcgtgcttct ccgacgcgtc catctgggcg aaggtgcgcc cgtcgccgtc
    27181 gtcgggcacg aacacggggt cgtacccgaa ccccccgtca ccgctcgggg agcgcgcgat
    27241 gcggccctcg cacactccct cggcgaacaa cggctcacgt cccggacggg cgaggcagat
    27301 gaccgtgcgg aaccgggccg tccggtgcgt cgagtcgact ccttcgaggc gttcgaggag
    27361 gagcttgacg ttgtcggcat cgactgcgtc ctcaccggcg aaccgcgccg aacgcacccc
    27421 cggcgctccg tcgagcgcgt cgacctcgag gccggtgtcg tcggcgatcg ccgcaccgtc
    27481 gacgtggtcg gccaccgccc gggccttcag ctcggcgttc gccgcgagcg tgtcgccgtc
    27541 ctcgacgaca tcgggcagct ccgccggccg cggcaacagc tccacgccgt caccgacggc
    27601 cgacgcgaag atctcctcga tctcccgcaa cttcttgggg ttggcggtcg cagcagcgac
    27661 ggcgagagcc accggacgct ccggatcagc gcttgtcgaa cgagcgggcc gagggcgcct
    27721 cggcgaccat ctcgcgttgc agcgcgacga tctcggtgat gccacccgtc gcgaggtcga
    27781 gcaacgacgt cagctcgtcg cggctgaacg gcacaccctc ggcggtgccc tgcacctcga
    27841 cgaggccacc cgcgccggtc atcacgacgt tcatgtcggt ctcggcgcgg gagtcctcga
    27901 cgtagggcag gtcgaggcgg ggctccccct cgatgatgcc gaccgagacg gcggcgcagt
    27961 ggtcgagcac cgggtgtcgg ctgacgagcc cggcctgcac catgcgggtg caggcgtcgt
    28021 gcagcgcgat ccacgcgccg gagatcgatg cggtgcgagt gccgccgtcg gcctgcaaca
    28081 cgtcgcagtc gaggtggatc tggcgctcac cgagcgcctt catgtcgacg acggcccgca
    28141 gcgaccggcc gatcagccgc tcgatctcgt gcgtgcggcc cttgggcccc ttgcgctccc
    28201 gaccgatgcg gtccggcgac gaaccgggaa gcatggagta ctccgaggtc acccatccct
    28261 tgccggtgtt cttcatccac cgcggcacgt cgagttcgac cgtggccgtg cagagcacct
    28321 tcgtgtcgcc gaacgacacg aggaccgagc cgtgggaggt ggtcgtgaag tcacgttcga
    28381 tggtgatggg gcggagttcg tcgttggcgc ggccgtctgg tctcatgggc gcgaccctag
    28441 ttctggcatg agacggcgcc ggcgaagccg actccgtggc gtgggcggtg caagactccc
    28501 ctgccgtggg gaccaccgac gtggacgacg acgcgacatg gctggcgacg ctcgacctgt
    28561 gcgacctcga ccggttcgcc cagggcttcc cgcacgacac gttcgaccgg ctccgccgcg
    28621 tcgcccccgt ctggtggcat ccaccgacgc ccaacactcc cgacggcgag gggttctggt
    28681 gcgtcacgcg ccacgccgac gtgctcaccg tggtgcgaga ccccgagacg ttctcctcgg
    28741 agtccggccc gggtcgtgac ggcaacggcg gtaccaccct cgtcgacatg cccgccgggt
    28801 tcgtcaccgg cgtgatgatg aacatgaccg acccgccggc ccaccggcgg atccggtcga
    28861 tcacgcaggc agcgttcacc ccgcgcacca tcgcccgcct cgacgactgg ttacgggcac
    28921 tggcggagca gctcgtcggc gacgcgctcg acgcctgttc cgacgagggc acggtcgacg
    28981 tcctcaccga cgtcgcggcc gagcttccgc tgcagacgat cgcccagttg atgggggtcc
    29041 cccagtccga ccgtcaccag ctcttcgagt ggacgagcac gatcctcgac tacggcgacc
    29101 ggtcgttgac cgagatcacc gacgagtacg ccgccgccgg gctcggactc cgcgcatacg
    29161 gcgacgccac gatcgccgat cggcgcgcca ccgtggcgcg tcacggtgcc gccatcgacg
    29221 gcgatctgtt gggccaggtg ttggcggccg acccgccgct cagccccgag gaggagcagg
    29281 cgttcttcag cctgctgttc acggccggct ccgagacgac ccgcaactcg atcgccggcg
    29341 gggtccacgc cttcgtcgaa cggcccggcg agtggcgtcg cctggtcgac gatcgctcgc
    29401 tgctcccgac agcggtcgag gagatcctgc gctggaccag tgcgaccgcc tacaaccgcc
    29461 gcaccgcaac ccgcgacgtg gagctcggcg gtcagcgcat cgcggcagga cagaaggtgg
    29521 cgcactggta cccgtcagcc aaccgcgacg agtcggtgtt cgccgacgcc ggtcggttcg
    29581 acgtgggccg ttcgcccaac ccgcaccttg cgttcggtca cggcatccac cactgcctcg
    29641 gcgccgccct ggcccgccgc gagctcaccg tcatgttcga ggtcctcgcc gatcgtgtcg
    29701 acgagttcga gtcgacgggt cccatcgaat ggggccgttc gaacaagcac acctcgattc
    29761 gccatcttcc cgtccgcctc gtcccgaaag gctcctcatg aaggtccacc tgaacgtccg
    29821 tgattacctc gaccgagcgc tccgggtgta ccccgatcgc actgcggtga tcgatgagcc
    29881 cgatcagccc gcggccccgc tcggtggggg ttcgctgact taccgcgagt tcggggaaca
    29941 cgcggcggcg ctcgccgccg gcctcgacgc gctgggcgtc ggcgtgggcg aacgcgtcgc
    30001 cgtcgtcagt cacaactcgg cgcgcctgct cgtggcgttc ttcggggtgt gcggatgggg
    30061 tcgggtgctc gtgccgatca acttccgcct ccatcccgag gaggtgcgct acatcgtcga
    30121 gcagagcggc gccacggtgc tcctcgtcga tcccgaactc gccgatgcca tggccgacat
    30181 cgagtgcgct caccggttcg tgatcggcga cgaatccgac gctgtgctgt tcaagttcga
    30241 caccgagccc cgggcctggg agcccgacga ggacgcgacg gcgacgctca actacacgtc
    30301 gggcaccacg gcgcggccga agggcgtcga ctgcacccac cgccagctct ggatgaacgc
    30361 cgccgtgttc ggttggcagg ccagcgtgaa cgaccgcgac gtgtacctcc acacactgcc
    30421 gatgttccac tgcaacggct ggggccaggt gttcagcgtg accggtatgg gcggcacgca
    30481 cgtcgtcctt cgcaaggtcg acggcgccga gatcctccgc cgcatcgaag ggcacggcgt
    30541 cacctacctg tgcgccgccc cggcggtggg ggccatggtc ctcgacgccg cggcggcgaa
    30601 cgccgacggc gagttcgacc cggttccggg ccgcgaccgc gtgcgcatgg tcatggccgg
    30661 cgctccgccg ccgacgcgga cgatcgagcg tgtggaggtc gaactcgggt gggagttcat
    30721 ccagatctac gggctcaccg agtcgtcgcc gctgttgacg atgagccgct cgcgctcgga
    30781 gtgggacgat ctcacgccgc gggaacgcgc cgtcaagctc ggccgcgccg gggcaccggc
    30841 gctcggcgtg gacctccaag tcgacgacca gggcgaggtg atcgctcgat cgaaccacat
    30901 cctcaacggc tactgggaca atcccgccgc caccgacgag gcgatcatcg acggctactt
    30961 ccacaccggc gacggcggca cgatcgacga cgaggggtat ctcacgatca gcgaccgcaa
    31021 gaaggacgtc atcatcacgg gtggtgagaa cgtgagctcg atcgaggtcg aggacgcgct
    31081 cttcaaccac gccgacgtcg ccgaggcgtg cgtgatcggg gtgccgtcgg acaaatgggg
    31141 cgagacgatc aaagccctcg tggtgctcgt gccgggaagc gacctcgcgg cgaagtcgca
    31201 cgaggaggtc gaggccgaac tgatcgcgtg gtgccggacg tcccacgccg gttacaagtg
    31261 cccgacctcg atcgaggtgc gcgacgatct gtcgcgcacg gccaccggca agctgcagaa
    31321 gttccgcctc cgcgccccct actgggaagg catcgagcgg caggtcaact gaccccctcg
    31381 gccctcaatg cccccggttc tgcgggtact tgtcatcacc tgctggggac aagtacccgc
    31441 gaaagcgggc cgaccggcgt aacgtccccg gccatggagc aacagcggct gaaggttcac
    31501 cggcgcaaga cccacctcga cggcgcggtg tacacagtgc tctcgcctcg agccgacgag
    31561 ggcccggtcc tgagcgtcga catcgtcgag gacggagcga ccatcacggg cgataccgcc
    31621 gccagtggcc tgctcggtca gctctgctgg gcgatggcgt accagcacac gccgtacacg
    31681 atcgctgtgg tcgacggcgt cgccggggcc ccggtggtcg ttgtcaacct cgacctcggc
    31741 gaggtgtcgg agggatcgct cacggcgctc ggcgcgctcc tcccgtggac cacgacgtcg
    31801 gacggcaccg tcaacctcaa cacccgcagt ctcgagtccg cggtcgccaa cgagccggcg
    31861 ttctacgccg accagaagaa ggccggcatc ggcgacatca agcaccagag ccggttcgtg
    31921 cggcaggtgg gcgacgtcct cgtgctcggc gccccggcca tcgtgctgaa gtcgtggggt
    31981 gtgcagctga gcacccccga cgatgcccgt ccgaagtggt tcctgacctc gccg
//
LOCUS       DAOSOA010000007        31319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_1467_length_31319_cov_6.304727, whole genome shotgun sequence.
ACCESSION   DAOSOA010000007 DAOSOA010000000
VERSION     DAOSOA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 31319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31319
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1467_length_31319_cov_6.304727"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKY13_01210"
     CDS             <1..287
                     /locus_tag="PKY13_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78567.1"
                     /translation="TNDTNVVAAASASLLWVAAMQPVGSLAFALDGVLVGAGRQRYLA
                     GAMVAAGAAFALTTVAVGRRDLAAIWVAVTTFMAVRALTGVWGVRRLTPT"
     gene            complement(294..857)
                     /locus_tag="PKY13_01215"
     CDS             complement(294..857)
                     /locus_tag="PKY13_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78568.1"
                     /translation="MRDRTTLLRRMVGLALVMAAVIVPAACSSSRDIESAGETKTTIR
                     DLGDDELVDEEALLSGSQGAKEIQKIIDRLVASNDACAILTQKDIKGLKIDATTLASS
                     SARKTLAAGVVKVYDHIIQIVPDAAIKPYLGTQRDTFVQVLDIVERYTANPTSKEGND
                     QIEALISGDKFVQAQSAVSGWTYQNCS"
     gene            complement(850..1527)
                     /locus_tag="PKY13_01220"
     CDS             complement(850..1527)
                     /locus_tag="PKY13_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78569.1"
                     /translation="MSVATRQLTALVALGLAMLLAAGCGATQRTELTLSGADAAPDDL
                     ARATTSPPAAAAAPDAGATTVVEPTTEPPAATAPTVEDAESAAALSAPASSAGATPTT
                     SRAAPPTTDPDARLLDEVEAAVGRQDICGVYAGLARFELATASTDRLIAQLTRIRTIM
                     LEASSFVTPALAADWAAVTEGMTGLLTSLEEDRAVGKVSKPSHYDDDDYREASGRVAD
                     WMGRNCA"
     gene            complement(1663..2187)
                     /locus_tag="PKY13_01225"
     CDS             complement(1663..2187)
                     /locus_tag="PKY13_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78570.1"
                     /translation="MGAGPVRSIVVAIVVVVAGVGCSDGDPKPTVAEVPTTTNPALAA
                     DLEPTPAPLSAEQIVAGLETAVGAADFCGLLRAIDTAEPDPDDRDGATDVYRALAAAT
                     RSARPFVPKELRTPWEDVVVGTDEAATAIEDHDGDVSDPELDAALTSTAMVEALESVE
                     RYQITSCPPPVAGG"
     gene            complement(2189..3688)
                     /gene="gatB"
                     /locus_tag="PKY13_01230"
     CDS             complement(2189..3688)
                     /gene="gatB"
                     /locus_tag="PKY13_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOT78571.1"
                     /translation="MTESVIAPSVGGTGEVFVTNRPQQPQNPAVPDGWEIVIGLEVHA
                     ELATYTKLFSGSLNEFGGEPNTNIDPVTLGLPGSLPVLNRQAVELAIRTGLALNCTVQ
                     RSVFARKNYFYPDMPKGYQISQYDLPINTDGWLELPDGTRIGIERAHLEEDTGKSSHM
                     GGASGRIHDAEYSLIDFNRAGVPLLEIVSRPDLRTADEAKAYVAELRSILMAVGASDG
                     KLEEGSMRVDCNVSVRRIGAGLGTRCEIKNVNSLRSLGRAVDYEARRQVDLITAGEAV
                     QQETRHWNDDSGRTSTLRTKEEATDYRYFPEPDLVVVDPSPEWIDAVRDALPLLPRER
                     RQRLVDLTGVAAENAAIVVDRGLDDLVVAAVEAGGDGARAVTHAEHNLSEDDAGLSPG
                     AFAALTRMEVDGAVTATQAKTVLAELVARGGDADPAAIAASLGFEAMASDENEALVEG
                     LIAAHPEDWAKFCAGENKVVGFFVGQAMKATQGKADGKAITALLNARKG"
     gene            complement(3685..5148)
                     /gene="gatA"
                     /locus_tag="PKY13_01235"
     CDS             complement(3685..5148)
                     /gene="gatA"
                     /locus_tag="PKY13_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOT78572.1"
                     /translation="MSDVTARAIAAQVRAGERSAVEVTEAALASIAAAEPHIAAFNLV
                     TADTARAAAADIDRRIAAGDDVGPLAGVPIAIKDNMCTRGIATTCSSKILEGWTPPYD
                     ATVVTRLAASGAVLVGKTNMDEFAMGSSTENSAFGVTRNPHDLTRVPGGSSGGSAAAV
                     AAGVVPIALGSDTGGSIRQPAALCGVVGVKPTYGSVSRFGLVAYASSLDQIGPFATNV
                     ADAAALLEAIAGHDPMDSTSIPAAFPAVSAHLDDGVAGLRVGLPVEFLGDGIAPDVRA
                     RVEAAAEALAAAGATVAETSVPAATVALSAYYLLAPAEASSNLSRFDGVRFGLRVEAP
                     TTPEMYDLSRTQGFGAEVKNRIMLGTYALSAGYYDAYYGTAQKVRTLIMRDFDAAYSQ
                     FDVLLTPTSPTTAFAIGAKTDDPLTMYMNDICTIPTNLAGHPAMSVPFGAGDDGLPIG
                     VQVLAPALGEVAMFRAAAVLEGAAPARLVPNLAGVAS"
     gene            complement(5145..5447)
                     /gene="gatC"
                     /locus_tag="PKY13_01240"
     CDS             complement(5145..5447)
                     /gene="gatC"
                     /locus_tag="PKY13_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOT78573.1"
                     /translation="MSERISRDDVAAVAKLARLRLSDAELDRATSQLGDILDHAADMA
                     SLDLEGVEPTSHPYPLSNVLRDDVALPSLDRDEVLAAAPAAEAGMFRVPPVIGEEP"
     gene            5573..5980
                     /locus_tag="PKY13_01245"
     CDS             5573..5980
                     /locus_tag="PKY13_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOT78574.1"
                     /translation="MAKYQFLTPEWVAAAKAIREEFEGESPAPAHSVRMNQIITEVPF
                     GDTDVKAFMDTSDGALNLDLGELENPDLTVTVDYETAKAIIVDGNPQAGMQAFMAGKI
                     KVQGDMTKMMALQAGGQDPVAAQVAERIQAITE"
     gene            complement(6050..7426)
                     /locus_tag="PKY13_01250"
     CDS             complement(6050..7426)
                     /locus_tag="PKY13_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT78575.1"
                     /translation="MTTPTTTRRSANPWVVTWTVLAGSFAVGINFTILAVSRPEIADD
                     LRADVSTLVWLISGPILANALFTATAGKLGDLYGHRRVYLLGIGGSVVFAMASAVAWD
                     SWSLIGFRVAGAVVGAAAAPASMAIINLMFPPAQRSRALGYWSLVAAGGPVIGLIIGG
                     PLVDAFGWRAIFLIQVPLLAIALAMAWRVLPETDRAEQTTFDVLGNVMLGVALLALLI
                     GVERGRSWGWGSAPTIGCFVLAAVASVLFIRVELRVDHPLLPVQYFRTRSFTMPIVVI
                     FFAQFGYMGGFILAPKLLDEIGHESATHISTLLIPRPLTFAIAGAAAGWFMRRLGMRN
                     IAAIGSMLVVVSLMMIAAVTDDLNTWVVVGAIALSGLGMGMVQPAIAASVANSVDDRD
                     LGVAGAAQQMSVQVATSLGMNLLDTVQAGLVATSGLAASYASSYHLGALLTLGGVVAA
                     WFVPKRTA"
     gene            complement(7587..9710)
                     /locus_tag="PKY13_01255"
     CDS             complement(7587..9710)
                     /locus_tag="PKY13_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT78576.1"
                     /translation="MSSRPFGQGSIVAGRYRIIAPLGSGASARVFLADDLQLGRRVAV
                     KVLHEALAEDRTFLKRFKAEAHSAAALNHPNVMHVYDSGDATVDEGPPLPFLVMEFVG
                     GGSLRAVLDSGPPFSPSQALVVGLDAARGLEYAHQHGFVHRDIKPANLLFGEEGRLRI
                     ADFGLARALAEATWTEPEGVLLGTAKYSAPEQALGKAVDGRSDVYSLALVLVEAVTGE
                     VPFARDSIQGTLLARTERDLEVPRSLGRLAPVLERAGRLDPDLRPDAGELVIAFLAAS
                     EDMDRPKPIGLPGAIPVQVLEMMVDQGKASAATDTDEHPVVDSETTTETDDGADDDGE
                     PDGLPDDITLLAAADQTAVVTLPVVRTAPTAPAASTASVATASTTPTDAAPAVETPTG
                     RVDIPLDDSTPEATAEATSRRWPWVLAAILLIGGLVGGAAYWWVAIRIPSHAVIDVGG
                     MPVKQATRELEDLGFKVTSSFVRKDGTEADEVVGQKPKVGTELEEGRTVELMVSLGNT
                     LVTVPVRDATMDEATVTTRLDEAGLVVGSRTNPFDETVPAGFLISSALSVAPDASGQV
                     PKETPVDIVISAGPQPRIIPPSLVGQPIAEVQRQLEAIQLKVNVTQEFSDEPIDTVLR
                     LNYSDNTEVPRDTVVEVATSKGPELFPIPNVLGQTGAQAGATLEAQGFRVAGVTGSPS
                     GKVVGIAPPAGELHPKGTSVQLLTN"
     gene            9856..10287
                     /locus_tag="PKY13_01260"
     CDS             9856..10287
                     /locus_tag="PKY13_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78577.1"
                     /translation="MPIKVKALLAVLVIAMVVIVVFVYDNMVTGPDQASLSKPEYVER
                     LIPDSGSEVLRQSTVGIDLKEGYDAYLVINGTAIRNDASGEDPDGLQKAPSLGTVEYA
                     PGPGRRIESLASPTQCVDAWVWKKLDGEATAKQVNWCFQVT"
     gene            10307..12628
                     /locus_tag="PKY13_01265"
     CDS             10307..12628
                     /locus_tag="PKY13_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HOT78578.1"
                     /translation="MTELPPNPLDPDENRFHLTPPRRSAAGARAAAVSLRRSIDDMGV
                     RRTARTLAVINQPEGFDCPGCAWPEAAPGERHHIEFCENGAKAVAEEATTDRVEPIFF
                     AEHSVADLATRSDYWLGQQGRLTHPMVKRPGATNYTTIEWDEAFALVADELRRLDDPD
                     EAVFYTSGRTSNEAAFAYQLLVRAFGTNNLPDCSNMCHEATGVALGAAIGIGKGSVRL
                     EDFVEADVILVVGQNPGTNHPRMLGTLGEAKAAGATIVAINPLPEAGLLNFKNPQRPS
                     GIIGSGTPLADLHLPIRLGGDQALFQLWSQRLLRRDAEHAGAIDREFIDGHTHGFEQF
                     AAHIDGLDADALLAATGLDGQLVDEAFEIVAGARRMIVCWAMGITQHRNAVETIREIT
                     NFAFLGGHIGRPGAGLCPVRGHSNVQGDRTMGIWEQPSEAFLDRLAAEFPTVDVPRRH
                     GLDVADAIAAFMDGRARTLFCLGGNFLRAIPDTAAAEEAIGRCRLTVHVSTKLNRSHA
                     ICGDTALILPTLGRTERDRQLGGDQFVTVEDSMGKVHRSTGVLQPASPHLRSEMAIVT
                     GVAEALLGRSSGIDWAAFRHDYSVVREHISRVVPGFEDFNERIMSPAGFELPHPPRDH
                     RTFPTPTGAANFAVTDVLAAGSIELIDPEGPVLLLQTLRSHDQYNTTIYGLDDRYRGV
                     HGGRLVVFVNEADIARLGLADGQFVDLVSHFDDVERRVRGFRIVAYPTPVGCVAAYYP
                     ETNPLIALGHRSIEAGTPASKSVPVTLEPTAFD"
     gene            complement(12653..13300)
                     /locus_tag="PKY13_01270"
     CDS             complement(12653..13300)
                     /locus_tag="PKY13_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT78579.1"
                     /translation="MTTAPIHAVFFDFGGVFIPSPFGAIGPAAERLAVPEADLVDAIF
                     GPYDADTDHPWHRLERGEISFEEARTAIGDLSEQRGLIRLDPMVALADLVIDRSIREF
                     MVDAVRDLRSQGIRTGIITNNIAEFGTHWKAMIPVDELFDDIVDSSAVGIRKPDAAIY
                     ALACERLGVEPANALFIDDYQGNVDGAVAAGLRAVCCGYTVESTEAALAELLALF"
     gene            complement(13297..15387)
                     /gene="ligA"
                     /locus_tag="PKY13_01275"
     CDS             complement(13297..15387)
                     /gene="ligA"
                     /locus_tag="PKY13_01275"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT78580.1"
                     /translation="MALPSDLPTEPAPRVAALVEQLRAHNAAYYERDAPTIPDAEYDE
                     LVRELKRLEEAHPELVVADSPASVTVGGAPSGAFAEITHRVPMMSLDNAMDVGELRAW
                     GDRTARKIESLGLDAGEIRYVCELKIDGLAMSIRYEHGEYVQAATRGNGRVGEDVTPN
                     VAGIAAVPDRLLGAPEVIEARGEVYLPLAAFEALNASQIEAGKPTYANPRNTAAGSLR
                     QKDASITAGRGLAFWSYQLGEVVGGPELATHSESLEWLRTLGLPVNPETQVVASLDEV
                     YEFCTHWTEHRHDLEYEIDGIVIKIDSFAVQTALGFTSKAPRWAIAYKLPPEERTTIL
                     RDIQVSIGGKGKATPFAVLEPVVVAGSTVGLATLHNEDQVRLKDVRPGDTVIVRKAGD
                     VIPEVVGPVLALRPADSVPWEFPEFCPCPHHTPLVRLEGEAKHQCVNIACPEQRWGRL
                     SHFASRGAMDIDGLGEKQVQLFIELGLLENVADIYDLDFDRILQHPGYKETSVNNLRR
                     AIEASKQRPLGNLLFGLTIPHLGSSGAETLAAGLGSLQNIMDATVDDIAAVEGIGPTI
                     ARSVVAFFEDAENRELVARLVAAGVNTVGPERSTLPQVLAGMAIVVTGGLEGFTRESV
                     AAAIKARGGKSPGSVSKKTTAVVLGEDPGASKLTKATELGIPVLDEAQFVHLLETGEL
                     PGSDVLEPENSETP"
     gene            complement(15424..15858)
                     /locus_tag="PKY13_01280"
     CDS             complement(15424..15858)
                     /locus_tag="PKY13_01280"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT78581.1"
                     /translation="MQHHVVRVGGIVQAPIETTWATLADARRYSTWALMSVSRLEREG
                     ASTPDGVGAIRNFGSGPMLSREEVVEFEPPNHLAYVLLAGLPISNYRADVRLTELGPN
                     SCRISWVGEFDARPVVGAVMQRFLHFVLGDFVRRLRRYAPTA"
     gene            15937..16476
                     /locus_tag="PKY13_01285"
     CDS             15937..16476
                     /locus_tag="PKY13_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HOT78582.1"
                     /translation="MSDLPVPVEHTVHARPRTDVQSERDEPAAGLVPDYVRRLAWVLD
                     DAIPIVGDRRIGIDGFLSFIPVVGEAAGFGLAGVIVLSGVRAGCSWVTIARMMFHAVG
                     ESVLGMIPLLGPVIAFFWKANQRNLRIIEKNLEDREATRRESWKVLLAAVALALITLA
                     VAFAGLVVAIYTIWRWISG"
     gene            complement(16481..16777)
                     /locus_tag="PKY13_01290"
     CDS             complement(16481..16777)
                     /locus_tag="PKY13_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78583.1"
                     /translation="MSTSGPTEPFGHGAANGDPLPPRPSHDVWGHQTGEDTAHLLDAS
                     GHVPRHQEPLARLVAEPHVITAADRRRATIARLAMVVVLLAAVAVAVWWVLSAT"
     gene            complement(16875..17816)
                     /locus_tag="PKY13_01295"
     CDS             complement(16875..17816)
                     /locus_tag="PKY13_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78584.1"
                     /translation="MGVPIRVTLPAGLATVIGALPHLNPFDASRVVRELLPGLPAGPT
                     IDLRFTEGDVDAAADGSSVASLDDDRLAAVRSFLGELRGRVEPVVLSLTGPVTIDLGL
                     MQRGVERERAASLSRGYVESWARAVIDLSTQMLPGAPLLLFLEEPGLANSMHPTFPLS
                     PNEIEGLVSGVVEPLSPDAMVGVQVSGRADWAMLLRTGISVLGAPISARLETAAAELG
                     RFLEAGGFIAWGAVPVDEPLGSGVERLWRRLSALWADLAQAGLDPLLLRERSIITPAA
                     GLGSFGLSQAERVIRLTGELSERVFNQVVGARLSIGA"
     gene            17863..18762
                     /locus_tag="PKY13_01300"
     CDS             17863..18762
                     /locus_tag="PKY13_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78585.1"
                     /translation="MRAHPVVWALRVCWLSLPFTTGDVVGHSLDGRSTAVTVVVATLA
                     WAGWATVLIATIVAHPVALTVLRILTPVAPVATVAALTVWAPDDLPTSGTVLGGIGLV
                     AALVATAAALSGVVADEYVDAASYGAERRFALRVPTAFLLGPVPIFWLTLVGGCLVGP
                     VLLAAQQWIGGVVLTVIGAAIAVPAVKAFHGLSRRWLVFVPAGVTLIDHIGLVDPVLF
                     PARQISAFGPAVEATTATDLTQQALGLVLEISFDSPVEVTARTSKVEGELQLLKAVLI
                     SPTRPGAVVAEADRRGLRTAVNA"
     gene            complement(18752..19813)
                     /gene="mnmA"
                     /locus_tag="PKY13_01305"
     CDS             complement(18752..19813)
                     /gene="mnmA"
                     /locus_tag="PKY13_01305"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOT78586.1"
                     /translation="MTLAPRSRVLVAMSGGVDSSVAAALLASQGHEVVGVTMKLWGGE
                     SDSGCCSVSDVDDARRVAQQLGIDHHVFNFGDDFEEFVVEPYVADHVAGLTPNPCIEC
                     NRHLKFDKLLRRADALGFDLVATGHHARRVERDGGYRIARGADRAKDQSYVLYPVAGP
                     ALARTLFPLGDMTKDEVRSLALELGLRTATKPDSQDVCFITRAQGGRSSFLGDRIPLR
                     SGRVVGTDGAELGTVDAVELVTIGQRRGIDVGGPGGRRYVVDVDGPAATVVVGTAEEL
                     LAPSVDVGDVVWADGPAAGAFAAQCSAHGRPLAATVSLLDDTTVRVDWAEPQRRVAPG
                     QSVVLYAGDEVVGGGVVRR"
     gene            complement(19810..20958)
                     /locus_tag="PKY13_01310"
     CDS             complement(19810..20958)
                     /locus_tag="PKY13_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT78587.1"
                     /translation="MSTAAVYLDHAASTPWRDVARAAYLETIELGAANASGAHRAARQ
                     ARALVDDARDALAGVLGVDAGDVVFTSGGTEADNLAVLGRHAAVGGRVLCSAVEHPAV
                     LEAVDEVGGKVVGTDSRGVIDLDQLAAVLDDDVTLVSVMTVNNEVGSVQPVAEAAELV
                     RRRAANALVHTDAVQALNWLDLRHVSPHVDLLSISAHKFGGPHGVGACVVRSGSVVRP
                     RHVGGGQERERRSGTLNVAGIVAMAAAAVECDAGRGAEVERVGALRDDLVARIVAGVP
                     DAIRSGVVDGAAEHLAAGFAHVCFEGVDSEALLFLLDDAGVAASAASACASGAQHPSH
                     VLAAMGVDRSTAQGSLRLTLGHTTTSTDVERAAAAVVAAVTRLRSFPR"
     gene            complement(20972..21733)
                     /locus_tag="PKY13_01315"
     CDS             complement(20972..21733)
                     /locus_tag="PKY13_01315"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT78588.1"
                     /translation="MSVTGTMGIITPEAVVLEFRSAGLATRTLAKAIDLAAQFMVLML
                     LLAVIGLAFGTAGAETAIVIGVALTVFVVVVLAPALCETFWNGRTPGKAILGLRVVTV
                     EGGPISFRHAIVRGLLQLVELPLGIAVFIALGNPRSQRLGDLAAGTFVISERAATELT
                     IPTLFFPPPGLEYYCAQLDVSRVDSEQFLLIRNFLLRVADLEALARYALAVRLATAAS
                     ALCTPPPPAGVPPEVYLVAVCSAYQQRHDGARSAT"
     gene            21778..>22496
                     /locus_tag="PKY13_01320"
     CDS             21778..>22496
                     /locus_tag="PKY13_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOT78589.1"
                     /translation="MDIDRFIVTHQASWNRLDELVRKGTRSVKKLSPQELDELLTLYQ
                     STSAHLSTARTRFDDVSLSNRLSRTIGGARGLIYRTKGRPGSAAARFFTETFPAAAWT
                     CRRAIGVAAFLLFAPALALGIWLSANGETRNAAIDPQTQELIAEREFEDYYKSEAAEG
                     WAFELFTHNIQVAVLSFGGGALGGIGGVLLLVSNGANLGVSAAVMHAYDKGALFWGLI
                     TPHGLIELTAIAVASGAGLKIA"
     assembly_gap    22497..22596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22685..24001)
                     /locus_tag="PKY13_01325"
     CDS             complement(22685..24001)
                     /locus_tag="PKY13_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT78590.1"
                     /translation="MLPTRRFALLAAAVGVGLWFLPGEFDGSLLWPLLAVNLLLLLVG
                     AADALAAVKPGLLVVTRDHPPVVVMGNRAHLTWTVQNTGPRAARFVLADELADSLGAD
                     TRRAGAHLAAGATATLTTSFRPTRRGRFTISEVVMRSTGPLGLMTRQHRRQVPTALRI
                     HPPFKSRDEAELRIRKGRILEVGLRSARGLGGGTEFEQLREYTADDEFRRVDWAASAR
                     AGKPIVRTYRPERNQTVMVLLDNGRLMAAQVDGVPRLEHAMDATIMLTAVASGLGDKI
                     GLVTFDQQVRSIVAPARHGHQVSRVTEAMFELEPVLAESDYQEAFRQTLVRFRRRALL
                     VILTDLAPGAVTDSLIPALPMILRNNLVVIGAVRDPEVAHWSEGVVVDAAQTFRRSAA
                     VAAVDDRERLVARLRGMGATVVDAVPGKLAPALADVYLHVKNTGRL"
     gene            complement(24026..25072)
                     /locus_tag="PKY13_01330"
     CDS             complement(24026..25072)
                     /locus_tag="PKY13_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT78591.1"
                     /translation="MSDTPFDPSRPPAPPQFSPPPAAPPAGAAVGDSSPARDAIMALR
                     SEIGKVVVGQEGTLSGLVAALLVRGHVLLEGVPGVAKTLLVKTMATALNLDFKRVQFT
                     PDLMPSDVTGQTILDVNDGSFRFREGPVFTNLLLADEINRTPPKTQASLLEAMEERQV
                     SVEGRSRLLPDPFIVIATQNPVEYEGTYPLPEAQLDRFMFKLLVGYPTFEQEAEVLTR
                     HHHGMNPHDIAAAGVRPVATAADLEQARLEVDRVQVEPVIIQYLVALCRATRETPAVQ
                     LGVSPRGAASLLHAAKAWAWLSGRPFLTADEVKAVAKPVLRHRLLVRPELELEGTTPD
                     GVLDSLLASVPAPR"
     gene            complement(25065..26255)
                     /locus_tag="PKY13_01335"
     CDS             complement(25065..26255)
                     /locus_tag="PKY13_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78592.1"
                     /translation="MTAPTASTAGGGGWKWLLPVLAAVVLTVIVLPSNEAGPLYDLGS
                     AQPDGYRGLRLLLEETGTTVDTLDADEIDAVAVGRFDVVYVPAGGAVDAALARQWRRY
                     VEAGGRLVLGSPSDELGARAVGDATSIDGLFVGGGSDLPAGVCTVDDLGEAGPIRAPF
                     LTQGVDAGDGDSCYGDERVALVVRQPTDRGEVVTLATPDLFTNEIMGAPEPEQPVGAI
                     PGNAVVAQRLLGSGAGAEPGRRLGVVTSGIADVAPTGEKSLTDFMSTGVKLGLLELGV
                     AFLFYAWARGRRHGRVVTEPAPVTIAGSAFVEAVGSLLERQGDVPRAAEVVREGQCRE
                     LARRLGLPRTAGRPELAAVVAARTGREPAAVLSLLTDPVDSDAGLVTLTRELDSLRQE
                     ALHV"
     gene            complement(26252..26878)
                     /locus_tag="PKY13_01340"
     CDS             complement(26252..26878)
                     /locus_tag="PKY13_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOT78593.1"
                     /translation="MLLRDDPTPAEVREKMREIFERSEFRRSKSWIERVLDWIGRHLS
                     PGEPSGGSGWGGSIGPVIGWVAIVLLAIGLAVLIAYVVRNFTPRAKKVKKEQLPDVEI
                     EELRTASEWASDADRLEASGDWKEAIRCRYRELVARLVDAGVAAPQAGRTTGELRLDV
                     AERAPEVGTEFSRATGLFELPWYADVPTGRDENAEFKALAARVLQEVS"
     gene            complement(26865..27788)
                     /locus_tag="PKY13_01345"
     CDS             complement(26865..27788)
                     /locus_tag="PKY13_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78594.1"
                     /translation="MDPLPPTVPTSVDWASPGSTVDAPRVRSAPTIFDEPPRPTRALV
                     ASPVSRLQPRTVGQLLDGGFEVLRFRFRTITILAATIVLPLYALPQALVAALGRVGDV
                     DVFGGSSPLGYLAQLGLWLATMLLGVAIGFLVSGWLVGADPTPADAFRFLRSRLGVAL
                     GAFVVALLLKVAGLVACGVGILFAVPVLSVLAPVVAAEGLGIVESVRRTIRLARRRLG
                     AVFGVGVLWAVSSSLVSAAAAGAAALVGELATGSTDGAEIAVQAVNVIGTVVLTVVQV
                     AVTVLVYVDLRVRTEGLDLELESMERFRAAP"
     gene            27948..28697
                     /locus_tag="PKY13_01350"
     CDS             27948..28697
                     /locus_tag="PKY13_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT78595.1"
                     /translation="MVADSGRYGRSFADVYDEWYADAPTDSLVEFVRARAAPGSDVLE
                     LGIGTGRVALALAAAGYRVTGIDSSPEMLDRLAAKPGAAAVAAILGDAADAAVYPSSG
                     VDAVLAVFNLLFNLTGADDQRRCLAAAAGALTPGGILVVEAFVPDPITERRRELVTRS
                     VDGARVVLIATDADPDEQTIDGGHIEITEHGTRLRPWRIRVTTPAGIDAMAAEAGLQL
                     VERFEDLAGTPFVDGESSHHVSVYRLAPPGV"
     gene            28707..29147
                     /locus_tag="PKY13_01355"
     CDS             28707..29147
                     /locus_tag="PKY13_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT78596.1"
                     /translation="MARLRVSTKLDATPDVVWADLADISSHVEWMRDAVAIRFTSDRH
                     QGAGTTFDCDTKVGPFRLTDRMEITAWEPGAAMGVRHTGLVTGDGVIRLRKLRRGRTR
                     LTWSERLVFPWWMGGPVGALAATPVLWLVWRGSMRNLRDRFAVD"
     gene            complement(29160..30035)
                     /locus_tag="PKY13_01360"
     CDS             complement(29160..30035)
                     /locus_tag="PKY13_01360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT78597.1"
                     /translation="MSYPSGPPQAVEQHCYRHTDRRAGVSCQRCNRPICPSCMISASV
                     GFQCPECAKQGAKQQRLVDTFRKETTPRLTYALIGVNVAVFLVGMGIGSSASGVGGSF
                     NGDFGLYRNAVANGEWWRLITSGFLHAGFLHLGFNMFALYSLGGFLERVIGPVKFALI
                     YGASLIGGSLGVTLLAGPLDLTVGASGAIFGVFAAFAVLQVSRGLSPWANGIGSTILL
                     NLFITFAVPGISIGGHLGGLVTGAVAGALLIGVNPRQALERQKQLPLFGAAVAGLAVV
                     LFAASIAAAHALPLV"
     gene            complement(30089..>31319)
                     /locus_tag="PKY13_01365"
     CDS             complement(30089..>31319)
                     /locus_tag="PKY13_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78598.1"
                     /translation="PLEVEIDGAPVICQTVLALAPEVPDVVPSGAILGEVPSSTGPVM
                     AYDALADPEAAAAFVAHVGDGIGTTAVTAIVDDPWLTTLELAQRWELTLYRRVQSGAH
                     PDVELTALLAPDGRGLTRPPAAVWRKNHHDLASVRRGVRRGESAAELSRASVTELLAR
                     RCQPRENPLDVIEPMGTLGQALAALHVGLADQLGTSDGSGSTLAAVLSSRLPRQLDEL
                     AIARVSATFRRLAYADDLGRFIRVHGNLDLSAAERTRGAWTFTRFGCCPDSDLVTDQE
                     PLSPLCDLAGLLHGVGRCAAAALDAAIAEREQTGDGGDDPDFYEQAARRELAVLAEAW
                     EERSVDAIIAGYTSNDEVHRLLPVERISRDALLTLFELELSVRDLVRSADRGPELLRI
                     PVEAVEDLAAASVRARW"
BASE COUNT         4881 a        10935 c        11042 g         4361 t
ORIGIN      
        1 tcaccaacga cacgaacgtg gtcgccgccg cctcggcgtc gctcctgtgg gtcgccgcga
       61 tgcagccggt cggcagcctt gcgttcgcgc tcgacggcgt cctcgtcggc gccggacggc
      121 agcgctacct ggccggggcg atggtggcgg ccggtgcagc gttcgcgctc acgaccgtcg
      181 ccgtgggacg ccgcgacctc gcggcgatct gggtcgcggt gacgacgttc atggcggtcc
      241 gcgcactcac gggggtatgg ggtgtccgac gactgacgcc cacctgatcg ggactagctg
      301 cagttctggt aggtccagcc ggagacggcg ctctgcgcct ggacgaactt gtcgccgctg
      361 atcagggcct cgatctggtc gttgccctcc ttcgacgtcg ggttcgcggt gtagcgctcc
      421 acgatgtcga gcacctgcac gaacgtgtcg cgctgggtgc ccaggtacgg cttgatggcc
      481 gcatcgggca cgatctggat gatgtggtcg tacaccttga ccacaccggc ggcgagcgtc
      541 ttgcgagccg acgacgacgc cagggtggtc gcgtcgatct tcaggccctt gatgtccttc
      601 tgcgtgagaa tcgcgcaggc gtcgttgctg gccacgagcc ggtcgatgat cttctggatc
      661 tccttcgcac cctgcgaccc gctgagcagc gcctcttcgt cgaccagctc gtcgtcaccg
      721 aggtcacgga tcgtcgtctt cgtctctccg gccgactcga tgtcgcgact cgacgaacag
      781 gcggcaggaa cgatgacagc cgccatgacc agtgcgagcc cgaccatgcg ccgcaggagc
      841 gtcgtacgat cacgcacagt tgcgccccat ccagtcggcg acccgacctg acgcctcgcg
      901 gtaatcgtcg tcgtcgtagt gcgagggctt cgacaccttg cccacggcac ggtcctcctc
      961 gagcgaggtc aacaaccccg tcatcccctc ggtcaccgcg gcccagtcgg ctgcgagcgc
     1021 cggggtcacg aacgacgagg cctccagcat gatcgtgcgg atgcgtgtca gctgggcgat
     1081 gaggcggtcg gtcgacgcgg tcgccaactc gaagcgggcg agaccggcgt acacgccgca
     1141 gatgtcctgg cggccgaccg cagcctcgac ctcgtcgagg agccgagcgt cggggtcggt
     1201 ggtcggtggc gctgcccgcg acgtggtcgg cgtcgcgccg gcggatgacg ccggcgctga
     1261 cagggcggcg gcggactcag catcctcgac ggtgggagcg gtcgcggcgg gtggttcggt
     1321 ggtgggctcg acgacggtgg tcgccccggc gtccggcgct gccgctgccg ctgggggaga
     1381 cgtggtggcg cgagcgagat cgtcgggcgc ggcgtccgcg ccgctcaggg tcagttcggt
     1441 ccgttgggtg gcgccacagc cggctgccag gagcatcgcc aacccgagcg ccacgagggc
     1501 ggtcagctgg cgcgtcgcta cggacatcgg gctccctcgt cgtggatcgg atcgggtggt
     1561 cggacgcccg aacgcgaccg gaaggggcgg aagggctggt tcagcatacg aaatgtcgct
     1621 ccgagggcaa gcgcgccgcc ctcgggtgcc ctcaggaacc cctcaaccgc ccgctaccgg
     1681 cggtgggcag ctcgtgatct ggtagcgctc gaccgactcg agggcctcga ccatcgccgt
     1741 ggacgtcagc gctgcatcca gctcggggtc ggacacgtcg ccgtcgtgat cctcgatcgc
     1801 cgtggcagcc tcgtcggtgc ccaccacgac gtcctcccac ggcgtacgca gctccttcgg
     1861 aacgaacgga cgagccgacc gggtcgccgc ggcgagcgcg cggtacacgt cggtggcgcc
     1921 gtcgcggtcg tcgggatcgg gctcggcggt gtcgatcgcc cgcaggaggc cgcagaagtc
     1981 ggccgctccc acggccgtct cgaggccggc cacgatctgc tcggccgaca agggtgccgg
     2041 tgtcggttcg agatcggcag cgagggcggg gttcgtggtc gtcggcacct cggcgaccgt
     2101 gggtttaggg tcgccgtccg agcagccgac cccggcgacg accaccacga tcgccaccac
     2161 gatcgagcgc accggaccgg cccccatgtc agcccttccg agcgttcagc agcgccgtga
     2221 tggccttgcc gtcggccttc ccctgggtgg ccttcatcgc ctgaccgacg aagaagccga
     2281 cgaccttgtt ctcgcccgcg cagaacttgg cccaatcctc gggatgggcg gcgatcagcc
     2341 cttcgacgag cgcctcgttc tcgtccgacg ccatggcctc gaacccgagt gacgcggcga
     2401 tggccgccgg atcggcgtct ccgccgcggg ccaccagctc ggcgagcacg gtcttggcct
     2461 gcgtggccgt caccgcaccg tcgacctcca tgcgggtcag cgcggcgaac gctccgggcg
     2521 acaggccggc atcgtcctcg gacaggttgt gctcggcgtg cgtgacggcg cgggcgccgt
     2581 cgccaccggc ttcgaccgcg gcgaccacga ggtcgtcgag gccccgatcg acgacgatcg
     2641 ccgcgttctc ggcggcgacg cccgtcaggt cgaccaggcg ctggcgtcgc tcccgcggca
     2701 ggagcgggag ggcgtcgcgc acggcgtcga tccactccgg cgacgggtcg accaccacga
     2761 ggtcgggttc ggggaagtac cggtagtcgg tcgcctcttc cttggtgcga agcgtggacg
     2821 ttcgaccgga gtcgtcgttc cagtgccggg tctcctgctg caccgcttcg ccggcggtga
     2881 tcaggtcgac ctgtcggcgg gcctcgtagt cgacggcgcg gccgaggctg cgcagcgagt
     2941 tgacgttctt gatctcgcaa cgggtgccga gacccgcgcc gattcggcgc acgctcacgt
     3001 tgcagtcgac ccgcatcgag ccttcttcga gcttgccgtc cgacgcgccc accgccatca
     3061 ggatcgaccg aagctcggcg acgtaggcct tcgcctcgtc cgcggtgcgg aggtcggggc
     3121 ggctcacgat ctcgagcagc ggcacaccgg cgcggttgaa gtcgatgagc gagtactcgg
     3181 cgtcgtggat ccggccgctc gcgccaccca tgtgggagga cttgcccgtg tcctcctcga
     3241 gatgggcgcg ttcgatgccg atccgggtcc cgtcggggag ctccagccaa ccgtcggtgt
     3301 tgatcggcag gtcgtactgg ctgatctgat agcccttggg catgtcgggg tagaagtagt
     3361 tcttgcgggc gaacaccgac cgctgcacgg tgcagttcag ggcgagtccg gtgcggatgg
     3421 cgagctcgac cgcctggcgg ttgagtaccg gcagactccc gggcaggccg agggtcacgg
     3481 ggtcgatgtt ggtgttgggc tcaccaccga actcgttgag cgagccgctg aacagcttcg
     3541 tgtacgtggc cagctcggcg tggacctcga gaccgatgac gatctcccag ccgtcgggaa
     3601 cggcagggtt ctgcggctgc tggggccggt tggtcacgaa gacctcgccg gtgccgccca
     3661 ccgacggtgc gatgacggac tcggtcacga cgccaccccc gccaggttgg gcaccaatcg
     3721 ggcgggcgcc gccccttcga gcaccgccgc cgcccggaac atggccacct cgccgagcgc
     3781 cggcgccagc acctggacgc cgatcggcag tccgtcgtcg ccggcaccga acggcaccga
     3841 catcgccggg tgcccggcga ggttggtggg gatcgtgcag atgtcgttca tgtacatcgt
     3901 cagggggtcg tcggtcttgg cgccgatggc aaacgccgtc gtcggtgacg tcggcgtgag
     3961 gagcacgtcg aattgggagt acgccgcgtc gaagtcgcgc atgatcagcg tgcggacctt
     4021 ctgggccgtg ccgtagtagg cgtcgtagta gcccgccgac agggcgtagg tgccgagcat
     4081 gatgcggttc ttgacctcgg caccgaaacc ctgggtgcgg gagaggtcgt acatctcggg
     4141 ggtggtgggt gcctccaccc gtagaccgaa ccgcaccccg tcgaagcgcg agaggttcga
     4201 cgacgcctcg gcaggagcga gcaggtagta ggccgacagc gccacggtcg cggcgggaac
     4261 cgacgtctcg gcgacggtcg ccccggcagc ggccagcgcc tccgcggctg cctcgacccg
     4321 ggcccggacg tcgggggcga tgccgtcgcc caggaactcg acggggagac cgacccggag
     4381 gcccgccacc ccgtcgtcga ggtgggcgct caccgcgggg aacgccgcgg ggatcgaggt
     4441 cgagtccatc ggatcgtgtc cggcgatcgc ttcgagcagc gccgccgcat cggcgacgtt
     4501 ggtggcgaac gggccgatct ggtcgagcga gctggcgtac gcgacgaggc cgaagcgcga
     4561 caccgagccg taggtgggtt tgaccccgac gacaccgcag agggccgccg gctggcggat
     4621 cgacccgccg gtgtcggacc cgagggcaat cggcacgacg ccggcggcga cggccgccgc
     4681 cgaaccgccc gacgaacccc ccggcacgcg ggtgaggtcg tgggggttac gggtgacccc
     4741 gaaggcggaa ttctccgtcg agctgcccat ggcgaactcg tccatgttgg tcttgcccac
     4801 gagcacggca cccgacgcgg cgaggcgcgt caccacggtg gcgtcgtagg gcggggtcca
     4861 gccctcgagg atcttcgaac tgcacgtcgt ggcgatgccc cgcgtgcaca tgttgtcctt
     4921 gatggcgatc ggcacgccgg cgagcgggcc gacgtcgtcc cccgcagcga tacggcgatc
     4981 gatgtccgca gcggcggcgc gggcggtatc ggccgtgacc aggttgaagg cggcgatgtg
     5041 gggttcggcc gcggcgatcg acgcgagcgc cgcctcggtc acctcgacgg cggaccgctc
     5101 gccggcccgg acctgcgctg cgatggctcg tgccgtcacg tcactcacgg ctcctccccg
     5161 atcaccggag ggactcggaa catgccggcc tcggcggcgg gcgccgcagc gagcacctcg
     5221 tcgcggtcga gcgacggcag cgcgacgtcg tcgcgcagca cgttgctcag tggatagggg
     5281 tgcgacgtgg gctcgacacc ctcgagatcg agcgaggcca tgtcggccgc gtggtcgagg
     5341 atgtcgccga gctggctggt ggcccggtcg agctcggcgt cgctcagacg cagccgggcg
     5401 agcttcgcga cggcggcgac gtcgtcgcgg gagatgcgtt cggacatagg ccgacgattc
     5461 taggtgtgct tcgagacgcg acagcccgtc acggctcgaa cagcgtggct acagtgacgc
     5521 ccgttcaccg catccgtgat ggaaatccgg agattgaaag aggaagccga agatggccaa
     5581 gtaccagttc ctgacccctg agtgggtcgc cgctgccaaa gcgatccgcg aagagttcga
     5641 gggggagtcc ccggccccgg cgcactcggt ccgcatgaac cagatcatca ccgaggtgcc
     5701 gttcggcgac accgacgtga aggcgttcat ggacacgtcc gacggagccc tcaacctcga
     5761 cctcggtgag ctcgagaacc ccgatctcac cgtcaccgtc gactacgaga cggccaaggc
     5821 gatcatcgtc gacggcaacc cgcaggccgg gatgcaggcc ttcatggccg gcaagatcaa
     5881 ggtccagggc gacatgacca agatgatggc gctccaggcc ggtggccagg atcccgtcgc
     5941 cgcccaggtc gccgagcgca tccaggcaat caccgagtag tacggctcac tcgctcggtt
     6001 ctgtcccgcg gatgagtgcg gatcgcactc atccgcggga cagaacgagt caggcggttc
     6061 gcttgggcac gaaccacgcg gcgacgacgc caccgagggt gagcagcgct cccaggtggt
     6121 acgaggacgc gtacgacgca gcgaggccgg acgtggcgac gagcccggcc tgcaccgtgt
     6181 cgagcaggtt catgccgagc gacgtcgcga cctgcaccga catctgttgc gccgcgccgg
     6241 caacgccgag atcccggtcg tcgacggagt tggcgaccga ggcggcgatc gcaggctgca
     6301 ccatgcccat gccgagaccc gacagggcga tcgcaccgac gacgacccac gtgttcaggt
     6361 cgtcggtgac cgcggcgatc atcatcagcg acacgaccac cagcatcgat ccgatcgccg
     6421 cgatgttgcg catccccagt cgacgcatga accagcccgc cgcagcgccg gcgatggcga
     6481 acgtcaaggg ccgcggaatc aacagcgtcg agatgtgtgt ggccgactcg tgcccgatct
     6541 cgtcgagcag tttgggcgcc aggatgaagc cgcccatgta cccgaactgc gcgaagaaga
     6601 tgaccacgat cggcatcgtg aacgaccggg tccggaagta ctgaaccggc agcaacgggt
     6661 ggtcgacgcg cagttcgacg cggatgaaca gcaccgacgc cacggcggcg aggacgaagc
     6721 agccgatcgt cggcgcgctc ccccaccccc acgaccgacc gcgctcgaca ccgatgagga
     6781 gcgccaggag cgcgaccccg agcatcacgt tgccgagcac gtcgaaggtc gtctgttcgg
     6841 cgcggtccgt ttcgggcagc acgcgccacg ccatcgccag cgcgatcgcg aggagcggaa
     6901 cctggatcag gaagatcgcc cgccacccga aggcgtcgac gagcggaccg ccgatgatga
     6961 ggccgatcac cgggccaccg gcggcgacca gcgaccagta gccgagcgct ctcgaccgtt
     7021 gcgccggtgg gaacatcagg ttgatgatcg ccatcgaggc cggtgccgcc gccgcgccga
     7081 cgaccgcgcc ggcgacgcgg aacccgatca gcgaccagct gtcccaggcc acggcggagg
     7141 ccatcgcgaa cacgacggac ccgccgatgc cgagcaggta gacgcgccgg tgtccgtaca
     7201 ggtcgccgag cttgcccgcc gttgcggtga acagcgcgtt cgcaaggatc ggaccggaga
     7261 tcaaccagac gagcgtcgag acgtcggcgc ggagatcgtc ggcgatctcg ggacgcgaca
     7321 cggccaggat cgtgaagttg atgccgacgg cgaacgagcc tgcgagcacg gtccacgtga
     7381 cgacccacgg gttcgcgctc cgccgggtgg tcgtgggggt ggtcaccgat cccaccgcgg
     7441 acgcggaacg acggagccgg ttggggcggc cacctcgtcg cggcggctgg ggaacaccac
     7501 gtaggcagac cgccccaacg agctccgtcg tcggacccga agactagggg cgcgattgcc
     7561 gaaagtgagg gatgctcggt cggccgtcaa ttggtcagca actggaccga ggtgcccttc
     7621 gggtgcagct cgccggcggg tggagcgatg ccgacgacct tgcccgacgg cgaccccgtc
     7681 acaccggcga cccggaagcc ctgtgcttcg agtgtggccc ccgcctgcgc tccggtctgg
     7741 cccaacacgt tggggatcgg gaacagctcc ggacccttcg acgtggcgac ctcgacgacg
     7801 gtgtcgcggg gcacctcggt gttgtcggaa tagttcaggc ggagcaccgt gtcgatcggc
     7861 tcgtcgctga actcctgggt cacgttgacc ttgagctgga tggcttcgag ctgccgttgc
     7921 acctcggcga tcggctgacc cacgaggctg ggcgggatga tgcggggctg aggcccggcc
     7981 gagatcacga tgtcgacggg ggtctccttg gggacctggc cgctggcatc gggagcgacc
     8041 gacagggccg aggagatgag gaacccggcc ggaaccgtct cgtcgaacgg gttcgtgcgg
     8101 ctccccacca cgaggcccgc ctcgtcgagg cgcgtggtga ccgtggcctc gtccatcgtg
     8161 gcgtcgcgga ccgggaccgt gacgagtgtg ttgccgagcg acaccatcag ctcgacggtc
     8221 ctgccctcct cgagctcggt gccgaccttc ggcttctggc cgacgacctc gtcggcctcc
     8281 gtgccgtcct tgcgaacgaa cgaactcgtc accttgaacc ccaggtcctc gagctcacgg
     8341 gtcgcctgct tgaccggcat accaccgaca tcgatcacgg catggctggg gatccgaatg
     8401 gccacccacc agtaggcggc gccgccgacg agcccgccga tgagcaggat cgcggccagc
     8461 acccagggcc agcgccgaga ggtcgcctcg gcggtcgcct cgggggtgct gtcgtcgagc
     8521 gggatgtcga cgcgacccgt tggcgtctcg accgcgggcg cggcgtcggt cggcgtcgtc
     8581 gaggcggtcg cgaccgacgc cgtcgatgcg gcgggggcgg tcggggcggt ccgaacgacg
     8641 ggaagggtca cgacggccgt ctggtcggcg gctgcgagga gggtgatgtc gtcggggagg
     8701 ccgtcgggct cgccgtcgtc gtcggccccg tcgtcggtct cggtcgtggt ctcactgtcg
     8761 accacggggt gctcgtcggt gtcggtcgcg gcggaggcct tcccctggtc gaccatcatc
     8821 tcgaggacct gcacgggaat cgccccggga agcccgatcg gcttcgggcg atccatgtcc
     8881 tccgacgccg ccaggaaggc gatgaccaac tcgccggcgt cgggccggag gtcgggatcg
     8941 agacgaccgg cacgctcgag gaccggagcg aggcgaccga gcgagcgggg tacctcgaga
     9001 tcgcgttcgg tccgggcgag cagcgtgccc tggatgctgt cgcgggcgaa cggcacctca
     9061 ccggtgacgg cctccaccaa caccagcgcg agcgaataca cgtcggagcg gccgtccacc
     9121 gccttgccga gcgcctgctc gggggccgag tacttggccg taccgagcag cacgccttcc
     9181 ggttcggtcc acgtggcctc ggcgagcgca cgggccaggc cgaagtcggc gatccggagc
     9241 ctgccctcct ccccgaacag caggttggcc ggcttgatgt cacggtgcac gaacccgtgc
     9301 tgatgggcgt attcgaggcc gcgggcggcg tcgaggccga cgaccagcgc ctgggacggg
     9361 ctgaacggcg gcccggagtc gagcacggcg cgaaggctgc cgccgcccac gaactccatc
     9421 accaggaacg gcaggggtgg gccctcgtcg accgtggcgt cgccggagtc gtagacgtgc
     9481 atcacgttcg ggtggttcag cgcggcggcg gagtgggctt cggccttgaa tcgcttgagg
     9541 aacgtgcggt cctcggcgag cgcctcgtgc aagaccttga cggcgacacg ccggcccaac
     9601 tggaggtcgt cggcgaggaa aacccgcgcc gaagcaccgc tcccgagagg ggcgatgatg
     9661 cggtatcgac cggcgacgat cgatccttgg ccgaacggac gcgacgacat gaccggttca
     9721 ccttagttct gcccgggagg gtgcccgtgg aggggccccg tggccgatgc cacgtctcga
     9781 cgcctcggcc acaccgatcg accggagcgg ccgaccgact gtgtgcagac ctcgacgacg
     9841 caccagaatg accacgtgcc catcaaggtg aaagccctcc tcgccgtgct cgtcatcgcg
     9901 atggtggtga tcgtcgtgtt cgtctacgac aacatggtga ccggtcccga tcaggcgagc
     9961 ctgtccaagc ccgagtacgt cgagcgcctg attcccgaca gcggcagcga ggtcctgcgc
    10021 cagtcgaccg tcgggatcga cctcaaggag ggctacgacg cgtacctcgt gatcaacggc
    10081 accgccatcc gcaacgacgc cagcggcgag gaccccgacg gcctccagaa ggcgccgagc
    10141 ctcggcaccg tcgagtacgc acccggcccc ggccgccgca tcgagtccct ggcgtcgccg
    10201 acgcagtgcg tcgacgcctg ggtgtggaag aagctcgacg gtgaggccac ggccaagcag
    10261 gtcaactggt gcttccaggt gacgtgatgg gtgagggcgg agcagcatga ccgagctgcc
    10321 cccgaatccc ctcgatcccg acgagaaccg gttccacctc actcccccgc gccgttccgc
    10381 tgccggcgct cgtgcggccg ccgtctccct gcgccgctcg atcgacgaca tgggcgtgcg
    10441 ccgcacggcc cgaacactcg ccgtcatcaa ccagcccgag gggttcgact gccccggctg
    10501 cgcgtggccc gaagcggcgc ccggcgaacg ccaccacatc gagttctgcg agaacggggc
    10561 caaggcggtc gccgaggagg cgaccaccga ccgcgtcgag ccgatcttct tcgccgagca
    10621 ctcggtcgcc gacctcgcga cgcgatccga ctactggttg ggccagcagg gtcgcctcac
    10681 ccacccgatg gtgaagcgcc ccggcgccac caactacacg acgatcgagt gggacgaggc
    10741 gttcgcgctc gtcgccgatg aactgcgccg actcgacgac cccgacgagg cggtgttcta
    10801 cacgtcgggt cgcaccagca acgaggcggc gttcgcctac caactcctcg tgcgagcgtt
    10861 cggcaccaac aaccttcccg actgctcgaa catgtgccac gaggccaccg gggtcgcgtt
    10921 gggtgcagcg atcggcatcg gcaagggctc ggtccggctg gaggacttcg tcgaggccga
    10981 cgtgatcctg gtcgtcgggc agaaccccgg caccaaccac ccacgcatgc tcggcacgct
    11041 cggcgaggcg aaagcagccg gcgcgacgat cgtcgccatc aatccgctgc ccgaggccgg
    11101 gctcctgaac ttcaagaacc cccaacgacc gagcggcatc atcggctcgg gaaccccgtt
    11161 ggccgatctg cacctcccga tccgcctggg cggcgaccag gcactgttcc aactgtggag
    11221 ccaacgcctc ctgcggcgcg acgccgaaca cgccggcgcg atcgaccgcg agttcatcga
    11281 tggccacacc cacggcttcg agcagttcgc tgcgcacatc gacgggctcg acgccgacgc
    11341 cctcctcgcc gccaccgggc tcgacgggca actcgtcgac gaggcgttcg agatcgtcgc
    11401 cggagcgcgc cgcatgatcg tgtgctgggc gatgggcatc acccagcacc gcaacgccgt
    11461 cgagaccatc cgggagatca ccaacttcgc gttcctcggc ggacacatcg ggcgccccgg
    11521 cgccggcctc tgcccggtgc gcggccactc caacgtgcag ggcgaccgca cgatgggcat
    11581 ctgggagcag ccctccgaag ccttcctcga ccgcctcgct gcggagttcc cgaccgtcga
    11641 cgttccccgg cgccacgggc tcgatgtggc cgacgccatc gccgcgttca tggacgggcg
    11701 cgcccggacg ctgttctgcc tcggcggcaa cttcctccgg gcgatccccg acacggcggc
    11761 ggccgaggag gcgatcgggc gctgccggct gaccgtccac gtgtcgacga aactcaaccg
    11821 ctcccacgcg atctgcggcg acaccgcact catcctcccc acgctggggc gcaccgaacg
    11881 cgaccgccag ctcggcggtg accagttcgt gaccgtcgag gactccatgg gaaaggtgca
    11941 ccggtcgacg ggggtactcc aaccggccag cccccacctg cggagcgaga tggcgatcgt
    12001 gacgggtgtg gccgaggcgc tcctcggccg ctcctccggc atcgactggg ccgcgttccg
    12061 ccacgactac tccgtggtgc gcgaacacat cagccgggtc gtgccgggct tcgaggactt
    12121 caacgagcgg atcatgtcac cggccgggtt cgagctcccc cacccgcctc gtgaccaccg
    12181 aacgttcccg acgccgacgg gcgccgcgaa cttcgccgtc accgacgtgc tcgctgccgg
    12241 ctccatcgag ctgatcgacc ccgagggccc ggtgctcctg ctccagacgc tccggtccca
    12301 cgaccagtac aacaccacga tctacgggct cgacgaccgg tatcgaggcg tccacggtgg
    12361 ccgcctcgtc gtcttcgtga acgaggccga catcgctcgc ctgggcctcg ccgacggcca
    12421 gttcgtcgac ctcgtcagcc acttcgacga cgtcgaacga cgggtgcgcg gcttccgcat
    12481 cgtcgcctac ccgaccccgg tcgggtgtgt cgccgcctac taccccgaga ccaacccgct
    12541 catcgcgttg ggacaccgct cgatcgaggc gggcaccccg gcctcgaagt cggtgccggt
    12601 caccctcgaa ccgaccgcct tcgactgagc gcctcaggcc gaggctggcg aatcagaaca
    12661 gcgcgagcag ttcggccagc gctgcctcgg tcgactcgac cgtgtagccg cagcagacgg
    12721 cacggagccc ggccgccacc gcgccgtcga cgttgccctg gtagtcgtcg atgaacaggg
    12781 cgtttgccgg ctccacgccg agccgctcgc acgccagcgc gtagatcgca gcgtcgggct
    12841 tgcgaatgcc gacggcggat gagtcgacga tgtcgtcgaa caactcgtcg acagggatca
    12901 tcgccttcca atgggtgccg aactcggcga tgttgttcgt gatgatgccg gtacggatgc
    12961 cctgtgacct caggtcgcgg acggcgtcga ccatgaactc gcggatgctg cggtcgatga
    13021 cgaggtcggc gagcgcgacc atcgggtcga ggcggatcag gccccgctgc tcggacagat
    13081 cgccgatggc ggtccgtgcc tcctcgaagg agatctcacc gcgttcgagt cgatgccacg
    13141 ggtgatcggt gtcggcgtcg tacggcccga agatcgcgtc gaccagatcg gcctcgggaa
    13201 cggccagccg ctcggcggcc ggcccgatcg ctccgaacgg ggacgggatg aacacaccgc
    13261 cgaagtcgaa gaacacggcg tggatcggag cggtggtcat ggggtctcgc tgttctcggg
    13321 ctcgaggacg tcggagccgg gaagctcccc cgtctcgagg aggtggacga actgtgcttc
    13381 gtcgagcacg gggatgccga gctcggtcgc cttggtgagc ttggaggcgc ccgggtcctc
    13441 gccgaggacg accgcggtcg tcttcttgga gacgctgccg ggcgacttgc caccccgggc
    13501 cttgatcgct gccgccaccg actcgcgggt gaagccctcg agcccacccg tcacgacgat
    13561 ggccataccg gcgagcacct gcgggagggt cgaacgctcg ggcccgacgg tgttgacgcc
    13621 agctgcgacg aggcgggcga ccaactcgcg gttctcggcg tcctcgaaga acgccacgac
    13681 cgaacgggcg atcgtgggcc cgatgccctc gaccgcggcg atgtcgtcga cggtcgcgtc
    13741 catgatgttc tgcaggctgc cgaggccggc agccagcgtt tcggcacccg atgaacccag
    13801 gtgcgggatc gtcagaccga agagcaggtt gccgagcggc cgctgtttgg acgcctcgat
    13861 ggcacggcgg aggttgttga ccgacgtctc cttgtagccc gggtgctgca ggatccggtc
    13921 gaaatcgagg tcgtagatgt cggcgacgtt ctcgagcagg ccgagctcga tgaagagctg
    13981 cacctgcttc tcgccaagcc cgtcgatgtc catcgcaccg cgggacgcga agtggctgag
    14041 gcgaccccac cgctgctcgg gacacgcgat gttgacgcac tgatgcttgg cctcgccctc
    14101 gagacgcacc agcggggtgt ggtgcgggca cgggcagaac tccggaaact cccacggcac
    14161 ggaatcggcc ggccgcaacg ccaggaccgg gcccacgacc tcggggatca cgtcgcccgc
    14221 cttccggacg atgaccgtgt cgcccggacg gacatccttg agacgcacct ggtcctcgtt
    14281 gtgcagcgtg gccagaccga cggtggaccc ggcgacgacc accggctcga gcactgcgaa
    14341 cggcgtggcc ttgcccttgc cgccgatcga cacctggatg tcacgcagga tcgtggtgcg
    14401 ttcctcggga ggcagcttgt aggcgatggc ccaccgcggc gccttcgacg tgaagcccag
    14461 cgccgtctgc accgcgaacg agtcgatctt gatgacgatg ccgtcgatct cgtactcgag
    14521 gtcgtgacga tgctcggtcc agtgcgtgca gaactcgtag acctcgtcga gcgatgcaac
    14581 gacttgggtc tcgggattga cggggaggcc gagggtacgg agccactcca gcgactcgga
    14641 gtgcgtcgcc agctcggggc cgcccaccac ctcgcccaac tggtacgacc agaacgccag
    14701 gccccggccg gcggtgatgc tcgcgtcctt ctgacgcagc gacccggccg cagtgttgcg
    14761 gggattggcg taggtgggtt tgcccgcctc gatctgcgac gcgttgaggg cctcgaacgc
    14821 agccagcggg aggtacacct cgccgcgggc ctcgatgacc tcgggcgcac caagcagccg
    14881 gtcgggcacg gcggcgatgc cggccacgtt gggggtgacg tcctcgccga cgcgaccgtt
    14941 gccgcgcgtg gcggcctgca cgtactcgcc gtgctcgtag cggatcgaca tcgccaaccc
    15001 gtcgatcttc aactcgcaca cgtaacggat ctcgccggcg tcgagcccca gcgactcgat
    15061 cttgcgggcc gtccggtcgc cccacgcccg aagttcgccg acgtccatcg cgttgtcgag
    15121 cgacatcatc ggaacccggt gggtgatctc ggcgaacgcg ccagacggtg ccccaccgac
    15181 ggtgaccgac gccggggaat cggcgaccac cagctcgggg tgcgcctcct cgagccgctt
    15241 caactcacgg accagctcgt cgtactcggc gtcggggatg gtgggtgcat cgcgctcgta
    15301 gtaagccgcg ttgtgggccc gcagctgctc gacgagggcg gcgacgcggg gcgcgggttc
    15361 ggtggggaga tcagaaggca acgccacggc ggagacacta ccggcggccg ctgacaccga
    15421 ctctcaggcg gtcggggcgt accggcggag ccgtcggacg aagtcgccca gcacgaagtg
    15481 gaggaaccgc tgcatcaccg cgccgacgac ggggcgggcg tcgaactccc ccacccagct
    15541 gatgcggcag ctgttcggac cgagctcggt gaggcgcacg tcggcccggt agttgctgat
    15601 cggcagcccg gcgaggagca cgtaggccag gtgattcggc ggttcgaact cgaccacctc
    15661 ctcgcggctc agcatcggcc cggaaccgaa gttgcggatg gctccgacgc catcgggcgt
    15721 cgacgcaccc tcgcgttcga gtcgagacac cgacataagc gcccacgtcg agtacctccg
    15781 ggcgtcggcg agggtcgccc aggtggtctc gatcggggcc tgaacgatgc cgccgacgcg
    15841 gacgacgtgg tgctgcatac gcaccagcat cggcggcgac cgggacgacc gcaacccgcc
    15901 ggggacggcc accgctggtc cactaacttg ccggtcgtga gcgaccttcc cgttcccgtc
    15961 gagcacacgg tgcacgcccg gccgcgtacc gacgtccagt ccgagcgcga cgagccggcg
    16021 gctggtctcg tccccgacta cgtccggcgc ctggcgtggg tgctcgacga cgcgatcccg
    16081 atcgtcggcg accggcgcat cggcatcgac gggttcctca gcttcatccc cgtcgtcggc
    16141 gaagccgctg gcttcggcct ggccggggtc atcgtgctgt cgggcgtgcg agcggggtgc
    16201 agctgggtga ccatcgcccg catgatgttc cacgccgttg gcgagtcggt gctcggcatg
    16261 atcccgctgc tcggcccggt catcgcgttc ttctggaagg cgaaccaacg caacctgcgc
    16321 atcatcgaga agaacctcga ggaccgtgag gcgacgcgac gcgagagttg gaaggtcctg
    16381 ctcgccgcag tggcgctcgc actgatcacg ctggccgtgg cgttcgccgg cctggtggtg
    16441 gcgatctaca ccatctggcg ctggatctcg gggtgacggg tcaggtcgcc gagaggaccc
    16501 accagacggc cacggccacg gccgccaaca ggacaaccac catcgcgagc cgggcgatgg
    16561 tggcgcgccg acggtccgct gcggtgatca cgtgtggttc ggcgacaagg cgcgccagcg
    16621 gctcctggtg acgggggacg tggccgctgg cgtcgagcag gtgggcggtg tcctcgccgg
    16681 tctggtgacc ccacacgtcg tgggacgggc gcggtggcaa cgggtcaccg ttcgctgcgc
    16741 cgtgcccgaa cggctcggtc ggtccggaag tcgacatggt ggtgccctct cgtccccggg
    16801 gtggcggccc gcttcaaatt tgaaggatga gggacggacg cgccgttggc aaggcccgag
    16861 aggccgtcgg atactcaggc gccgatggag aggcgggcgc cgacgacctg gttgaacacg
    16921 cgctcggaca gctcgccggt gagccggatg acgcgttcgg cctggctgag gccgaagctt
    16981 cccaacccgg cggccggggt gatgatcgag cgctcgcgca gcagcaacgg gtcgaggccg
    17041 gcctgcgcca ggtccgccca cagggccgac agacggcgcc acaaccgctc gacaccagag
    17101 ccgagcggct cgtcgaccgg gacggcgccc caggcaatga acccgcccgc ctcgaggaat
    17161 cgaccgagct cggcggcggc ggtctcgagt cgcgccgaga tcggcgcgcc cagcacactg
    17221 atgccggtac gcagcagcat ggcccaatcg gcgcggccgc tgacctggac gcccaccatg
    17281 gcatcgggcg acaacggttc gacgacaccc gacaccaggc cctcgatctc gttgggcgac
    17341 aacgggaacg tcgggtgcat cgagttggcc aggcccggct cctcgaggaa caggaggagc
    17401 ggcgcgccgg ggagcatctg cgtcgacagg tcgatcaccg ctcgagccca cgactcgacg
    17461 tagccccgcg acaacgacgc cgcgcgttcg cgctcgacac cgcgttgcat gaggccgaga
    17521 tcgatggtga ccgggccggt caacgagagc accacgggct cgacccgccc gcgcagctca
    17581 ccgaggaacg aacgcaccgc agcgaggcga tcgtcgtcga gcgatgcgac cgacgagcca
    17641 tcggcggcag cgtcgacgtc accctcggtg aaccggagat cgatggtggg accggccggg
    17701 aggccgggca acaactcgcg gacgacccgc gacgcgtcga acggattgag gtgcggcagt
    17761 gcgccgatca ccgtcgccag gcccgcgggc aacgtgacac gaatcggtac ccccatgggt
    17821 cttgcttagc gatgagtgcc tcgcgtacgc aagtcttggg gcatgcgcgc gcaccctgtc
    17881 gtctgggccc tccgggtctg ttggttgtcc ctcccgttca cgacgggcga cgtcgtggga
    17941 cactcgctcg acggcaggtc gaccgccgtc accgtggtcg tcgccacact cgcgtgggcg
    18001 ggttgggcaa cagtgctcat tgcgacaatc gtcgcccacc cggtggcgct gaccgtgttg
    18061 cgcatcctca cgccggtggc accggtggcg acggtcgcag cgctgacggt gtgggcgccc
    18121 gacgacctcc ccacatcggg caccgtcctc ggcgggatcg gcctcgtcgc cgcgctcgtc
    18181 gccactgcag cggccctctc gggagtcgtc gccgacgagt acgtcgacgc agcgtcctac
    18241 ggcgccgagc gacggttcgc gctgcgggtg ccgacggcgt tcctgctcgg cccggtgccg
    18301 atcttctggc tcaccctcgt cggaggctgc ctggtcgggc cggtgctgct cgcagcgcag
    18361 cagtggatcg gcggcgtggt actcacggtg atcggcgccg ccatcgcggt gcccgccgtc
    18421 aaggcgttcc atggcctgtc gcgacggtgg ctcgtgttcg tgccggccgg cgtcaccctg
    18481 atcgaccaca tcgggctggt cgatcccgtc ctgttccccg cgcggcagat ctcggccttc
    18541 gggcccgccg tcgaggcgac gacggcaacc gacctcaccc agcaagcgct cggcctcgtg
    18601 ctcgagatct cgttcgacag tcccgtcgaa gtgacggcgc ggacctccaa ggtggagggc
    18661 gagctgcaac tcctcaaggc agtgctcatc agccccacgc gtccgggcgc cgtggtcgcc
    18721 gaagccgacc gacgcggtct ccgcacggcc gtcaacgcct gacgacgcct ccaccgacga
    18781 cctcgtcgcc cgcgtagagc accacactct gcccgggagc gacccgccgc tgcggctcgg
    18841 cccagtcgac cctgaccgtg gtgtcgtcga ggagcgacac ggtcgccgcc agcgggcgac
    18901 cgtgggcact gcactgcgcg gcgaacgcgc cggccgccgg gccgtccgcc cagaccacgt
    18961 cgccgacgtc gaccgacggc gccaacagct cctcggcggt tccgaccacc acggtggcgg
    19021 ccgggccgtc gacgtcgacc acgtaccggc gcccaccggg tccgccgacg tcgatgccgc
    19081 ggcgttgccc gatcgtcacc agctctacgg cgtcgacggt gcccagctcg gcgccgtcgg
    19141 taccgacgac acgacccgag cgcagcggaa tgcggtcgcc gaggaacgag ctgcgcccgc
    19201 cctgcgcccg tgtgatgaag cacacgtcct ggctgtcggg tttggtggcg gtgcggagcc
    19261 ccagctcgag cgccagcgag cggacctcgt ccttggtcat gtcgccgagc gggaacagcg
    19321 tgcgcgccaa cgccgggccg gccacggggt acagcacgta gctctggtcc ttggcccggt
    19381 ccgcgccacg ggcgatccgg tacccgccgt cgcgttcgac acggcgggcg tggtggccgg
    19441 tcgcgacgag atcgaacccg agcgcgtcgg cccggcgcag cagcttgtcg aacttgaggt
    19501 gacggttgca ctcgatgcac gggttcgggg tgagaccggc gacgtggtcg gcgacgtacg
    19561 gctcgacgac gaactcctcg aagtcgtcac cgaagttgaa cacgtggtgg tcgataccca
    19621 actgctgggc gacgcgacgg gcatcgtcga cgtcggagac cgagcaacag cccgagtcgc
    19681 tctcgccgcc ccacagcttc atcgtgacgc cgacgacctc gtgcccctgg gacgcgagaa
    19741 gggcggcggc aacggagctg tcgacgccac cggacatggc gacgagcacc cgtgaacgcg
    19801 gcgcgagtgt catcggggaa agctccgcag gcgcgtgacc gcagcgacga ccgcagccgc
    19861 ggcccgctcg acatcggtgg acgtggtcgt gtggccgagg gtcagccgca acgaaccctg
    19921 ggcggtcgac cggtcgacgc ccatcgcggc gaggacgtgg gaggggtgct gagcgcccga
    19981 tgcgcaggcc gacgccgccg aggcagcgac gccggcgtcg tcgaggagga acagcagcgc
    20041 ctcgctgtcg accccctcga agcagacgtg ggcgaacccg gcggcgaggt gctcggccgc
    20101 gccgtcgacg accccggagc ggatcgcatc gggcacaccg gcgacgatcc gcgccaccag
    20161 gtcgtcacgc agggcaccga cacgttcgac ctcggcgccg cgaccggcgt cgcactcgac
    20221 ggccgccgct gccatggcga cgatgccggc cacgttgagc gtgccgctcc gacgctcgcg
    20281 ctcctgccct ccaccgacgt gccgggggcg gacgaccgaa ccggaccgca cgacgcacgc
    20341 gccgaccccg tggggtccgc cgaacttgtg ggccgagatc gacagcaggt cgacgtgagg
    20401 ggaaacgtgg cgcaggtcga gccagttcag cgcctgcacg gcgtcggtgt gcacgagggc
    20461 gttggcagcg cgacgccgca cgagttcggc ggcctcggcg accggctgga ccgaaccgac
    20521 ttcgttgttg accgtcatca ccgagaccag ggtgacgtcg tcgtcgagga ccgcagcgag
    20581 ctgatcgagg tcgatcaccc cgcgggaatc ggtgccgacc accttgccgc ccacctcgtc
    20641 gaccgcctcg aggacggccg gatgctcgac cgccgaacac agcacccggc caccgacggc
    20701 ggcgtgacgc cccagcacgg ccaggttgtc ggcctcggtg ccgccgctcg tgaacacgac
    20761 gtcaccggcg tcgaccccga gcacaccggc gagcgcatcg cgggcgtcgt cgaccaacgc
    20821 ccgcgcctgc cgagcggcgc ggtgcgcccc gctggcgttg gccgcaccga gctcgatggt
    20881 ctcgaggtag gccgcccgcg ccacgtcgcg ccagggcgtc gacgccgcat gatcgaggta
    20941 gacggcagcg gtgctcaccg cgccaggcta cctatgtggc tgatcgggcc ccgtcgtgac
    21001 gctgctggta ggccgaacac acggcgacca ggtagacctc gggcggcacc ccggcggggg
    21061 gcggtggggt gcacagggcg ctcgccgcgg tggcgagccg cacggcgagc gcgtagcggg
    21121 cgagcgcctc gagatcggca actctcagga ggaagttgcg gatcaggagg aactgctcgg
    21181 agtcgacgcg gctgacgtcg agctgcgcgc agtagtactc gagtcccggc ggcgggaaga
    21241 agagcgtggg gatggtgagc tcggtcgccg cccgctcgct gatcacgaac gtgccggccg
    21301 cgaggtcgcc gagtcgttgg ctacgcggat tgccgagtgc gatgaagacg gcgatgccga
    21361 ggggcagctc cacgagctgg agcagtccac gaacgatcgc gtggcggaac gagatcggcc
    21421 ccccttccac cgtgacgacc cggagcccga ggatcgcctt gcccggcgtc cgcccgttcc
    21481 agaaggtctc gcagagcgcc ggcgccagga cgaccacgac gaagacggtc agcgcgaccc
    21541 cgatcacgat cgccgtctcg gcgcctgcgg tgccgaacgc gaggccgatc acggccagga
    21601 gcagcatgag caccatgaac tgggccgcca ggtcgatggc cttggcgagt gtgcgcgtcg
    21661 ccaggcccgc cgaacggaac tcgaggacca ccgcctccgg cgtgatgatc cccatggtgc
    21721 ccgtcacgct cacagcgatc acggtactgg cggtgtcggg tcgtacgatg gagccccatg
    21781 gacatcgacc ggttcatcgt gacgcaccag gcgtcgtgga accgactcga cgagctcgtc
    21841 cgcaagggca cccggtcggt caagaagctg tcaccccagg agctcgacga gctcctgacg
    21901 ctctaccaat cgacgagcgc gcacctcagc accgctcgca cccggttcga cgacgtcagc
    21961 ctctcgaacc gactgagccg cacgatcggc ggcgcccggg gactcatcta ccgcacgaag
    22021 ggccggcccg ggtcggcggc ggcccggttc ttcaccgaga cgttccctgc cgcggcctgg
    22081 acctgccgcc gggccatcgg ggtggccgcg ttcctcctgt tcgcaccggc gctcgcgctc
    22141 ggcatctggc tgtccgccaa cggtgagacc cgcaacgccg cgatcgaccc gcagacccag
    22201 gaactgatcg ccgagcgcga gttcgaggac tactacaagt ccgaggccgc tgaggggtgg
    22261 gccttcgagc tgttcaccca caacatccag gtggcggtgc tctcgttcgg cggcggcgcg
    22321 ctcggcggca tcggtggggt cctgctgctc gtgtcgaacg gggcgaacct cggggtgagc
    22381 gcggccgtga tgcacgccta cgacaagggc gcgctcttct ggggtctgat caccccccac
    22441 ggcctcatcg agctgacggc gatcgccgtg gcgtcggggg ccggcctcaa gatcgcnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgg gccgcttcgg cgagccgagc
    22621 atggccgacc tgcgcgccgc gcaggaggaa ggggcgctga ccgaggcggc ctcggcctga
    22681 cggcctacaa ccgacccgtg ttcttcacgt ggaggtagac atccgcgagg gccggcgcga
    22741 gcttgcccgg caccgcgtcg acgaccgtgg ctcccatccc gcggaggcgg gcgacgagcc
    22801 gctcgcggtc gtcgaccgcg gccacggcgg cgctacggcg gaacgtctgt gcagcatcga
    22861 ccaccacccc ctcggaccag tgggcgacct cgggatcgcg caccgcaccg atgaccacga
    22921 ggttgttgcg caggatcatc gggagcgccg ggatcagcga gtcggtcacc gcgccgggag
    22981 ccagatcggt gaggatcacg agcagcgccc gtctccggaa gcgaacgagc gtctgacgaa
    23041 aggcctcctg gtagtcactc tcggccagca cgggctcgag ctcgaacatc gcctcggtca
    23101 ctcgtgacac ctggtggcca tggcgcgccg gcgccacgat cgaccgcacc tgctggtcga
    23161 aggtgaccag gccgatcttg tcgcccagac cgctcgccac ggcggtgagc atgatcgtcg
    23221 cgtccatcgc gtgttcgagg cggggcaccc cgtcgacctg cgcagccatc aggcggccgt
    23281 tgtcgagcag caccatcacg gtctggttcc gttcggggcg gtaggtgcgc acgatcggct
    23341 tgccggcccg ggcggaggcg gcccagtcga cgcggcggaa ctcgtcgtcg gccgtgtact
    23401 cccgcaactg ctcgaactcg gtgccgccgc ccagaccgcg ggccgaacgc agccccacct
    23461 cgaggatccg tcccttccgg atgcgcagct ccgcctcgtc acggctcttg aagggcgggt
    23521 ggatgcgcaa cgccgtcggc acctgccggc gatgctggcg ggtcatgagt ccgagggggc
    23581 ccgtgctccg catgacgacc tcggagatcg tgaaccggcc gcgacgcgtc ggacggaacg
    23641 acgtggtcaa cgttgcggtc gcaccggcgg ccaggtgcgc tccggcccga cgggtgtcgg
    23701 cgcccaacga atcggcgagc tcgtcggcga gcacgaaccg ggcggcgcgg ggcccggtgt
    23761 tctgcacggt ccacgtcagg tgcgcgcggt tgcccatcac caccacgggc ggatggtcgc
    23821 gggtgacgac gaggagtccc ggcttcaccg cagcgagcgc gtcggccgca ccgacgagga
    23881 ggagcagcag gttgactgcc agcaacggcc acaggagcga cccgtcgaac tcgcccggca
    23941 ggaaccagag gccgacgccg acggcggcgg ccaacaaggc gaatcttcgg gtggggagca
    24001 tcggggcgaa cgactcggag ccggatcagc ggggtgccgg gaccgacgcg aggagcgagt
    24061 cgaggacacc gtcgggcgtg gtgccctcca gctcgagctc ggggcgcacg aggaggcggt
    24121 ggcgcagcac cggcttcgcg acagccttca cctcgtcggc ggtgaggaac gggcgaccgg
    24181 acaaccacgc ccacgccttg gcggcgtgca gcagtgacgc ggcgccacgg ggtgacacgc
    24241 ccagctgcac cgccggagtc tcccgggtcg cccggcacag ggcgacgagg tactggatga
    24301 tgaccggctc gacctgcacc cggtcgacct cgagccgggc ctgctcgagg tcggcggcgg
    24361 ttgccaccgg tcgcaccccg gcggcggcga tgtcgtgggg attcatccca tggtggtggc
    24421 gcgtgagtac ctcggcctcc tgctcgaacg tcgggtaccc caccagcagc ttgaacatga
    24481 aacggtcgag ctgggcctcg ggaagtgggt aggtgccttc gtactccacc gggttctgcg
    24541 tcgcgatcac gatgaacggg tcgggcagca ggcggctgcg accctccaca gacacctgcc
    24601 gctcctccat ggcctccagg agggacgcct gcgtcttcgg gggcgtgcgg ttgatctcgt
    24661 cggcgaggag caggttcgtg aacacgggac cttcgcggaa ccggaacgaa ccgtcgttca
    24721 cgtcgaggat cgtctgaccg gtcacgtcgg acggcatgag gtcgggcgtg aactgcaccc
    24781 gcttgaagtc gaggttgagc gcggtcgcca tcgtcttcac gaggagcgtc ttggcgacac
    24841 cgggcacccc ttcgaggagc acgtggccgc gcacgagcag ggcggcgacc aagccggaca
    24901 gggtgccctc ctgcccgacg acgaccttcc cgatctcgga ccgcagcgcc atgatcgcgt
    24961 cacgcgccgg cgacgagtca ccgacggccg ctcccgcggg cggcgctgcc ggcggcggcg
    25021 agaactgcgg cggggcgggt ggacggctgg gatcgaaggg ggtgtcagac atgcaatgcc
    25081 tcctggcgta gcgaatcgag ttctcgggtg agcgtgacga gcccggcgtc ggagtcgacg
    25141 ggatcggtca acagcgacag gaccgcggcc ggctcccgac cggtgcgggc ggcgacgacc
    25201 gcggcgagtt cgggccgccc ggccgtgcgg ggcagtccga gccggcgggc cagctcgcgg
    25261 cattggccct cgcggaccac ctcggcggct cgggggacgt caccttgccg ttcgagcagg
    25321 ctgcccacgg cttcgacgaa cgcggagccg gcgatcgtga ccggcgccgg ctccgtcacg
    25381 actcgtccgt gtcgacggcc gcgggcccac gcgtagaaca ggaaggcgac gccgagctcg
    25441 agcagcccga gcttgacgcc ggtcgacatg aagtcggtca gcgacttctc gccggtcggt
    25501 gcgacgtcgg cgataccgga cgtgacgacg ccgagacgtc ggccgggttc ggcgccggcg
    25561 ccgctaccca ggagccgctg ggcgacgacg gcgttgccgg gaatggcacc caccggctgc
    25621 tccggttcgg gcgcgcccat gatctcgttg gtgaataggt ccggggtggc cagcgtgacg
    25681 acctcgccgc ggtcggtcgg ctgtcggacg acgagggcga cgcgttcgtc gccgtaacag
    25741 ctgtcgccgt cgccggcatc gacaccctgg gtgaggaacg gcgcccggat cgggccggcc
    25801 tcgccgaggt cgtcgacggt gcacacgccg gccggcaggt ccgatccgcc gccgacgaac
    25861 agcccgtcga tcgacgtcgc gtcgccgacg gcacgcgcgc cgagctcgtc ggacggcgag
    25921 ccgagcacca accgcccacc ggcctcgacg tagcggcgcc actgccgcgc caacgcagcg
    25981 tcgaccgctc ctccggccgg gacgtagacc acgtcgaacc gcccgaccgc gacggcgtcg
    26041 atctcgtcgg cgtcgagcgt gtcgacggtc gtccccgtct cctccaacaa cagacggaga
    26101 ccgcggtagc cgtcgggttg cgctgaaccg aggtcgtaga gcggaccggc ctcgttcgac
    26161 gggagcacga tcaccgtgag cacgaccgcg gcgagcaccg gcaacaacca cttccaccca
    26221 ccgccgcccg cggtcgacgc ggtcggggcc gtcacgagac ctcctggagg acacgggcgg
    26281 cgagcgcctt gaactcggcg ttctcgtccc ggccggtcgg cacgtccgcg taccacggga
    26341 gctcgaacag gcccgtggcc cgcgagaact cggtccccac ctccggagcg cgttcggcca
    26401 cgtcgaggcg aagctcaccg gtggtgcgcc cggcttgcgg cgccgcgacc ccggcgtcga
    26461 cgagccgggc gaccagctcc cggtaccggc agcggatcgc ctccttccag tcgccagacg
    26521 cctcgaggcg atcggcgtcc gacgcccact cggacgcggt ccgcagctcc tcgatctcga
    26581 cgtcggggag ctgctccttc ttcaccttct tcgcccgcgg tgtgaagttg cggacgacgt
    26641 atgcgatgag cacggccagc ccgatcgcca agaggacgat cgcgacccac ccgatgacgg
    26701 gtccgatcga gccgccccat cccgagccac cggacggctc gccgggcgac aggtggcggc
    26761 cgatccagtc gagcacccgc tcgatccacg acttcgatcg ccggaactcc gagcgctcga
    26821 agatctctcg catcttctcc cgaacctcgg ccggcgtcgg atcgtcacgg agcagcacgg
    26881 aaccgctcca tcgattcgag ctccaggtcg aggccttccg tgcggacccg gaggtcgacg
    26941 tacacgagca ccgtcacggc cacctggacg acggtcaaca ccacggtgcc gatgacgttc
    27001 accgcctgca cagcgatttc ggcgccgtcg gtcgaacctg tcgcgagttc cccgacgagc
    27061 gccgctgcgc cagcggcggc ggcactcacc agcgacgacg acaccgccca caacacgccg
    27121 acgccgaaca ccgcaccgag gcggcggcgg gcgaggcgga tcgtgcggcg caccgactcg
    27181 acgatgccga gaccctcggc ggcgaccacg ggggcgagca ccgacagcac cggtacggcg
    27241 aacaagatgc ccaccccgca ggccacgagg cccgccacct tcagcaacaa cgcgacgacg
    27301 aaggcgccga gcgcgacgcc gagccggctg cgaaggaacc ggaacgcgtc cgccggcgtg
    27361 gggtcggcac cgacgagcca ccccgacacg aggaagccga tcgcgacacc gagcagcatc
    27421 gtcgccagcc agaggcccag ttgagcgagg tacccgagcg gcgacgaccc cccgaagacg
    27481 tcgacgtcgc cgaccctgcc cagcgccgcc acgagcgcct gcggcagcgc gtacagcggc
    27541 agcacgatcg tcgccgcgag gatcgtgatc gtccggaacc ggaaccggag cacctcgaac
    27601 cctccgtcga ggagctggcc gaccgtccgg ggttggagcc gcgaaacggg actcgccacc
    27661 agcgcccggg tcggacgcgg tggttcgtcg aagatcgttg gagcggaacg gacccgcggc
    27721 gcatcgacgg tgctgccggg cgacgcccag tcgacggagg tggggacggt cgggggaagc
    27781 ggatccacgg ggctcggtac gggtgggcga gccgcctgac ggcactcgct cgtggggcga
    27841 gccaccattc tgcacggtcg ccgccgaaaa tctgttcatc ggccgaggtg cccacacgtc
    27901 ggagatggaa tcgtcgcagt cggagggccg tgccagactg cccgccgatg gtcgcggatt
    27961 ccggacggta cgggcgcagc ttcgccgacg tgtacgacga gtggtacgcc gacgccccga
    28021 ccgactcgct cgtcgagttc gttcgtgcgc gggccgcccc cgggtccgac gtactggagc
    28081 tggggatcgg caccggccgc gtcgcactcg cgctcgccgc cgccgggtat cgggtcaccg
    28141 gcatcgactc ctcccccgag atgctcgatc ggctggcggc gaagccgggt gccgccgcgg
    28201 tcgcggcgat cctgggcgac gccgccgacg ctgcggtgta cccgtcgagc ggggtcgacg
    28261 ctgtactcgc ggtcttcaac ctgctcttca acctgaccgg cgccgacgac cagcgccgct
    28321 gtctcgccgc ggccgccggc gcactcaccc cgggcgggat cctcgtggtc gaggcgttcg
    28381 tgcccgaccc catcacggag cggcggcgcg aactcgtcac ccgctccgtc gacggcgcgc
    28441 gggtcgtgct catcgccacc gacgccgacc ccgacgagca gacgatcgac ggcggccata
    28501 tcgagatcac cgagcacggc acccgcctcc gcccgtggcg cattcgggtc acgacaccgg
    28561 ccggcatcga cgcgatggcg gcggaggccg gactgcagct cgtcgagcgg ttcgaagacc
    28621 ttgcgggtac tcccttcgtc gacggcgaga gctcccacca cgtctccgtc taccggctcg
    28681 ccccgcccgg ggtgtaagca tccgccatgg cccgactccg tgtgtccacc aagctcgatg
    28741 ccacacccga cgtggtgtgg gccgatctcg ccgacatctc gtcgcacgtc gagtggatgc
    28801 gcgacgccgt cgcgatccgc ttcacgtccg accggcacca gggcgccggc accacgttcg
    28861 actgcgacac caaggtcggg cccttcaggc tcaccgaccg gatggagatc accgcctggg
    28921 aacccggagc ggcgatgggg gtccgccaca ccggcctggt caccggcgac ggggtgatcc
    28981 ggctgcggaa actgcggcgc gggcgcaccc gcctcacgtg gagtgaacga ctcgtgttcc
    29041 cctggtggat gggcggcccg gtcggcgcac tcgccgccac gcccgtcctc tggctcgtgt
    29101 ggcgcggctc gatgcgcaac ctgcgggacc gattcgccgt cgactgacga cgggagcgct
    29161 cagaccagcg gcaaggcgtg ggccgcggcg atcgacgccg cgaacaggac caccgccaac
    29221 cccgcaacag cggcgccgaa gagcggcagt tgcttctggc gctcgagcgc ctgacgggga
    29281 ttcacgccga tgagcagcgc gccggcgacg gcgcccgtga ccaggccacc gagatggcca
    29341 ccgatcgaga tgcccggcac cgcaaaggtg atgaacaggt tgagcaggat ggtgctcccg
    29401 atcccgttgg cccacgggct gaggccgcgg ctcacctgca gcacggcgaa tgccgcgaac
    29461 acaccgaaga tcgcgcccga cgcgccgacg gtgagatcga gcggtcccgc caacagggtc
    29521 acgccgagcg acccgccgat gagcgacgcc ccgtagatga gcgcgaactt caccgggccg
    29581 atcacccgct cgaggaaccc accgagcgag tagagcgcga acatgttgaa tccgaggtgg
    29641 aggaacccgg cgtgcaggaa cccgctggtg atcagacgcc accactcgcc gttcgcgacg
    29701 gcgtttcggt acaggccgaa gtcgccgttg aacgatcccc cgacaccgct ggcgctactg
    29761 ccgatgccca tgccgacgag gaacaccgcg acgttgacgc cgatcaacgc gtacgtgagg
    29821 cgcggcgtcg tctccttgcg gaacgtgtcg accagccgtt gctgttttgc accctgcttg
    29881 gcgcactcgg ggcactggaa gccgacggat gcgctgatca tgcacgacgg gcagatcgga
    29941 cggttgcagc gctggcagct gacgccggcg cggcgatcgg tgtggcggta gcagtgctgc
    30001 tcgacggcct ggggcggccc ggagggatac gacacgtcgt cagcctaccg acgagcgccg
    30061 tggcgcccgt ggtgtcggct gagagcgctc accaccgcgc ccgaacgctc gctgccgcga
    30121 ggtcctcgac cgcctcgacg gggatgcgca ggagctcggg gccccgatcg gccgaacgaa
    30181 cgaggtcgcg caccgacagc tccagttcga acagcgtcag cagtgcgtcg cgggagatgc
    30241 gctcgacggg gagcagccgg tgcacttcgt cgttcgacgt gtagccggcg atgatcgcgt
    30301 cgaccgaccg ctcctcccac gcctcggcga gcacagcgag ttcgcggcgg gccgcctgct
    30361 cgtagaagtc gggatcgtcg ccaccgtcac cagtttgttc acgttcggcg atcgcagcgt
    30421 cgagggcagc ggccgcgcac cggcccacgc cgtggaggag gccggcaagg tcacagagcg
    30481 gcgacaacgg ctcctggtcg gtcaccaggt cggagtcggg acagcacccg aatcgagtga
    30541 acgtccaggc gccccgagtg cgttcggccg cggacaagtc gagattgccg tgcacccgga
    30601 tgaatcgccc gagatcgtcg gcgtacgcga gccggcgaaa cgtcgccgac acgcgggcga
    30661 tcgccagctc gtcgagctgc cggggcagac gcgatgacag caccgcagcg agcgtcgacc
    30721 cgctgccgtc ggacgtgccg agttggtcgg cgaggcccac gtgcaaggcc gccaacgcct
    30781 gaccgagcgt tcccatgggc tcgatcacgt cgagcgggtt ctcccggggc tgacaccgcc
    30841 gggcgagcag ctcggtcacc gacgcccgac tcagctcagc ggcggactcc ccccgtcgga
    30901 cccctcggcg gaccgaggcg aggtcgtggt ggttcttgcg ccacaccgct gcgggcgggc
    30961 gggtgaggcc acggccgtcg ggtgccagca gggcggtgag ctcgacgtcg gggtgcgcac
    31021 ccgactgcac ccggcggtac agggtgagtt cccagcgttg ggccagttcg agcgtcgtca
    31081 gccacgggtc gtcgacgatc gcagtgaccg cggtggtgcc gatgccgtcg cccacgtgcg
    31141 ccacgaacgc cgccgccgct tcggggtcgg cgagggcgtc gtaggccatg accgggccgg
    31201 tgctcgacgg aacctcaccc aggatcgccc cggacgggac gacgtcgggc acctcgggag
    31261 cgagtgccag caccgtctgg cagatcaccg gggcgccgtc gatctcgacc tcgagcggc
//
LOCUS       DAOSOA010000008        28849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_1635_length_28849_cov_5.889942, whole genome shotgun sequence.
ACCESSION   DAOSOA010000008 DAOSOA010000000
VERSION     DAOSOA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 28849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28849
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1635_length_28849_cov_5.889942"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKY13_01370"
     CDS             complement(<1..582)
                     /locus_tag="PKY13_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOT78599.1"
                     /translation="MTFGVVLTIAVALAFDYTNGFHDAANSVATVVSTRVLSPQLAVF
                     WAAFFNFIAFAVFGTEVANTIAKDVVDPLSNVLTVGAIFAGLLGAIGWNLATFYLGLP
                     SSSSHALVGGMAGAAVARGGFDVLVADGMRKIAAFIILSPLIGLALGLIISLIILWCC
                     YRVKNVDGLNRGFRRGQLLSAAAFSLGHGANDAQ"
     gene            complement(605..1219)
                     /locus_tag="PKY13_01375"
     CDS             complement(605..1219)
                     /locus_tag="PKY13_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOT78600.1"
                     /translation="MRFRLVPTDDKFFDLFIDTAHNVHNCALLLQKMVENLVDSGAQR
                     DAILAAERKGDDLTRDVLARLHTSFVTPFDREDIHNIAERLDDVTDDMFHVADLLHML
                     KIREPMPEMAELCDVLVRMSAANVSLFERFESMKDLDSLLMEMGQLESEGDRIYRRAI
                     ARLFAEHDTMTVLKWKDIISATENAIDRLEDVSDVVESVIVKNA"
     gene            complement(1378..3600)
                     /locus_tag="PKY13_01380"
     CDS             complement(1378..3600)
                     /locus_tag="PKY13_01380"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOT78601.1"
                     /translation="MPAVTRVLPWRRHQLPAAEKVGPLVEQFRNAHPKASSELITRAY
                     EVAEAAHEGQMRRSGEPYITHPLAVATIVAHFGLDETTIVAALLHDVAEDTDFTLADI
                     EKEFGAEVAALVDGVTKLDRIHFDSKEAQQAAAIRKMLVAMADDIRVLVIKLCDRLHN
                     MRTIAALPADKQEKTAWETMDVYAPLAHRLGMGELKQQLEDLCFAALHPKRYAEIDHM
                     VATRSPERELYLTQVEEEVRSRLAEVQIQAEVTGRPKHMWSIYEKMVVRGKEFDDIHD
                     LVGMRVIVASEKDCYAALGSIHSIWTPVAGRFKDYIATPKFNLYQSLHTTVIGPQAKA
                     VEVQIRTREMHQRAESGIAAHFSYKAGDGGGGVEDLSWLNRMVDWQHEMSDPAEFMAN
                     LKSDLDQDEVYVFTPKGDVHNLPLGATPVDFAYAIHTEVGHRCIGARVGGRLVPLEHH
                     LTSGDTVEIFTSKVEGAGPSLDWLDFVATRSAQQKIKQWFSRERREDALESGRELLAK
                     ALRSESSHVQKLATDELLAEIAGEFHTPSLDALHTAIGEHRIEASDVVAKVVSQMRGA
                     DNGRSEVISSGSARNRRTGTGSVGVTVEGQVNELVRIARCCTPVPGDPIIGFMTRGRG
                     VSIHRTDCEIGASLARGHGERLIDVTWDDHSTGTFSVVVELKALDRSRLLQDVTHMLS
                     DQQVNIVSCLTSTGPDRVSKMRFEFELGDPSHLEAILSGLRRIDSVYDAYRVLPGGGN
                     "
     gene            complement(3616..4137)
                     /locus_tag="PKY13_01385"
     CDS             complement(3616..4137)
                     /locus_tag="PKY13_01385"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOT78602.1"
                     /translation="MDPADLRHHIRDIPDYPQPGVMFRDITPLLGDPAAFSASIGALA
                     EPHLGQVDLVAGVEARGFMFAAPVAHRLGVGFVPVRKPGKLPHTTVTETYELEYGTDS
                     LEIHTDAVAAGQRVLVVDDVLATGGTAAATVALLTSMGADVVAASFLIEIAPLDGRSR
                     LGDLPVHTVITYA"
     gene            complement(4180..5340)
                     /gene="secF"
                     /locus_tag="PKY13_01390"
     CDS             complement(4180..5340)
                     /gene="secF"
                     /locus_tag="PKY13_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOT78603.1"
                     /translation="MGTLSRLYRVQTDFNFTKVWRIMIVISSILVLIGVGSLATRWLN
                     LSIDFRGGAVWEVPSKTMSTGDAEDVLASFDKENGAKIQSVTDANGRRIVRVQAEAAK
                     NIAESDKIADALAEKAGIKVTEIATNTVGPSWGASLTKDAARALVVFFIVIAAYMSWQ
                     LEWRMAVSALLAVVHDVILTLGFYSVFGFEVTPATVISVLTILGFSLYDTIVVYDRVQ
                     ENAARYDRTGRFTYNAIMRRSLNQVLMRSLNTTFVAILPVVAILVIGGIVYGQATMLD
                     FSLALLVGLVAGAYSSIAIASPLVVWLKEREPRYAKIRQRARDRGAEVEADWIPVAEV
                     ALAGSPVSSSPYVAFDGDSAPSTAPKVSPLATKAAQYQREHPPRPRKQGKRR"
     gene            complement(5340..7502)
                     /gene="secD"
                     /locus_tag="PKY13_01395"
     CDS             complement(5340..7502)
                     /gene="secD"
                     /locus_tag="PKY13_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOT78604.1"
                     /translation="MKKKKGLVPLALVTVLSLGSCLAVILADFTPNLGLDLQGGVSVV
                     LQPVKAGKKQTKVSQEALEQTQRVIETRVNAIGVGEPDITIQGTTIVVQLPGIKDQKR
                     VLDLVGKTAELRFRPVLAAPTAAPPKDADEQIAALREELKIPEGVTVQQIVDEENAAA
                     TAADPTVTDPTATTVPTDPTATTAVDPAATTVAPTTAAPTTTAASGAGSGGKSSMKGA
                     AARRQSTTTTAAEAATTTTAAPTATTTTAPGTVTTTTVYVPKNQWGIDVTDPSFAKLY
                     QLDQAVNNDAEKATAPADDKADAEVVLLGQPDSDGTRVKYKLGPTLLTGRAIETASAG
                     LANGKWVVNPVYKDGKDGIDLFNKAAALCNAGAPECPGIASGEGGAQVGQLAVVLDGE
                     VISAPAINAASFSRDQIQISGSFTEQSSKDLALALRYGSLPLELEPQTVQTVSATLGS
                     GALRSGIIAGLIGLGVVVLYVIAYYRLLGAITVGSLILTAALLWAVVGLLGHYSGLAL
                     SLAGIVGIIVSIGVSLDSSVVYFENLKEDVMHGRTLRSAVDRSFDQAWGTIIKADVSS
                     LIGAIILYVLSVGPVKGFAFYLALSTVIDLVLAYMFIRPATVLAARSKLGLRPALFGI
                     PMPATTPAGAGGATGRSARTDASTRAAAGSKPVATGAIVADEPEVEPEPEPEPEVAPE
                     SEIEAVEAATDAEPDAYEPDAGEVVATDDDPTNEGGER"
     gene            complement(7752..8141)
                     /gene="yajC"
                     /locus_tag="PKY13_01400"
     CDS             complement(7752..8141)
                     /gene="yajC"
                     /locus_tag="PKY13_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT78605.1"
                     /translation="MNALLSIIAAEQQSGGSFIIFIPLIALMGYLLIVSPRKQKKKQA
                     EMMSSLKVGDEVMTTSGIYGTISYLEDNIAHLVVDTDVVIRVSKTSVNRLGGAAASSD
                     EVIDLDEVAGETDAGSDDAPSSKSKKS"
     gene            complement(8171..9286)
                     /gene="tgt"
                     /locus_tag="PKY13_01405"
     CDS             complement(8171..9286)
                     /gene="tgt"
                     /locus_tag="PKY13_01405"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOT78606.1"
                     /translation="MKLHIDIEATDGAARTGRVRTARGEFRTPVFMPVGTRGAVRTVT
                     TADVERLGAEIILGNTYHLMLRPGADVVADLGGLHRFEDWSGHMLTDSGGFQIFSLNP
                     KVDDDGATFKSTYDGSTHRVTPEEAVRIQELLGADIQMVLDVCTALPAKPGQLRRAVE
                     RTADWAARARASHGRHGDESIVERQALFGIVQGGTDADLRAESAQRTVELDFDGYAVG
                     GLSVGETRDVMLPALEAAVAHLPADQPRYFMGLGDPVGIVEAVARGIDMMDCVLPTRL
                     ARHGTLLSDGGRVSIRRKEFQRSDEPIDPSCPCEVCRRYSKGYLRHLMTVGEPTGATL
                     CTLHNTAWLLRLMDRIREAIAAGRLDALRAEVAAVWG"
     gene            complement(9283..10353)
                     /gene="queA"
                     /locus_tag="PKY13_01410"
     CDS             complement(9283..10353)
                     /gene="queA"
                     /locus_tag="PKY13_01410"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOT78607.1"
                     /translation="MKAQEFDYELPERAIATVGMEPRDSARLLVDTAGRGDGTAEGLV
                     DAVVGDLPDHLRAGDVFVVNDTRVLPARVRFTRSTGGSGEVLFLTAIEDGWWDALVRP
                     SAKLPPGTVVAVDDRFSLEFGADLGEGRRIVCPLVDGAPAAGSTLLDALERVGEMPLP
                     PYLGDAHLDDPDRYQTVFANRPASAAAPTAGLHLTDALLDRIRAKGVQIARVELVVGL
                     GTFRPMTTDDVEDHVMHHESYRVPPTSWDAIERARSESRRIVAVGTTVVRALESAAAT
                     GELAGSTDLFIRRPYDWQVVDVLMTNFHLPRSTLLVMIDAFVGPRWRDIYAAALDRGY
                     RFLSFGDAMLLERERSTESAST"
     gene            complement(10364..11242)
                     /locus_tag="PKY13_01415"
     CDS             complement(10364..11242)
                     /locus_tag="PKY13_01415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT78608.1"
                     /translation="MKTACSLANFTWEGGTAAIGPKIAEVAQIADEAGFSSLTAMDHW
                     FQIEIAGPPENAMLEGYSVLAFAAGLTKRINLQLLVTGVTYRHPGLLAKTVTTLDVLS
                     GGRATLGIGAAWFEEEHVALGVPYPSTAERFERLDETLAIYDQMCSDDEGPFEGKHYQ
                     LARTLNSPQPLSGRPPVMIGGMGPNKTLRLAARYAQAVNWFPIGTGAFQGLKATLAKH
                     CETEGTDVDSIRLTMMAMHPDCSDPTAVDEFVAQMAEYAALGVDEVFLSPSTADPVPT
                     VHAIGEILVPRLAELG"
     gene            complement(11339..12424)
                     /gene="ruvB"
                     /locus_tag="PKY13_01420"
     CDS             complement(11339..12424)
                     /gene="ruvB"
                     /locus_tag="PKY13_01420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT78609.1"
                     /translation="MRDELLDPEFNSLDAVVADGLDVRDVDGQAAIDAANLRPRDLDE
                     FVGQAELKHRLRIILEAARRRGQAADHLLFAGPPGLGKTTLAGIVANEMGVHLHVTSG
                     PTLERAGDLAAILTKLGEGDVLFIDEIHRLSRTVEEVLYPAVEDFQLDIVLGKGPAAR
                     SIRLDLPRFTLVGATTRTGLITGPLRDRFGLVARLEFYDVADLESIVRRAAGIVAADI
                     DAGGAHEIARRSRGTPRIANRLLRRVRDYAEVEADTRIDREVASDGLAVFGVDELGLD
                     KVDRAVLTALCDLFGGGPVGLSTLAIAVAEPTETVEDVYEPFLIQQGLLMRTPRGRVA
                     TPAAWRHIGLVPPPSASASAADPSLFD"
     gene            complement(12435..13046)
                     /gene="ruvA"
                     /locus_tag="PKY13_01425"
     CDS             complement(12435..13046)
                     /gene="ruvA"
                     /locus_tag="PKY13_01425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT78610.1"
                     /translation="MIGSLRGTLLDRTGTDVTVEVGGMGYRMSTTPATSGALGVEGSE
                     VFVWVHHHIREDAQTLYGFAGREERDVFETLIGTHGVGPALGLAILSTHSPRALRLAL
                     ATDDVSALCLVPGVGRKTAQRLLVELKSRLDGSGDVDLRALAADGASPSAATGPSAHA
                     DVREALVGLGYGNEEIAGVVGQLPLDGDASDLLRQALQKLAVA"
     gene            complement(13057..13548)
                     /gene="ruvC"
                     /locus_tag="PKY13_01430"
     CDS             complement(13057..13548)
                     /gene="ruvC"
                     /locus_tag="PKY13_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT78611.1"
                     /translation="MFVLGIDPGLSRCGYCVLSVGPRGRSRAVAVGVLTTPVDLSTPK
                     RLAILQRDVRELLDDYPPSAVAIERVLFQVNVSTAMSVGQASGIVMAEAAGRGCDVIE
                     YSPNQVKEAVAGYGSADKEQMQEMVRRLLGHSAVLRPADAADAAAVALCHVAYAPSMV
                     TVR"
     gene            13689..14714
                     /locus_tag="PKY13_01435"
     CDS             13689..14714
                     /locus_tag="PKY13_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78612.1"
                     /translation="MGHRFGDVQALDELTFDVPARRITVLLGPNGAGKTTAIRSITGA
                     LSPKAGDISVFGTDPRGEGDQVRPRCGVVSAKPALYDRLSGYDNLLYSAQLYDVPAAG
                     LRQKIVAAAERFGIADALDHQVGGYSTGMKTRLALSRSTLHDPDLLLFDEPTSGLDPE
                     SSQAVLDLIREMTNDGKTVVMCTHLLAEAEGLADHIVVMEAGHALCTGTPDELTERYL
                     PAAMVVLDAEAPDQLDRLAHMTGVIGYDRTDVARIELDGFGRVPDLVLSLARDGVRLT
                     RVEPHVPTLEDLYFAVRRERTELLAGRPLPTLDTPDPQGFGRANARSAGVSTTETVGA
                     GHEEVSA"
     gene            14711..15535
                     /locus_tag="PKY13_01440"
     CDS             14711..15535
                     /locus_tag="PKY13_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT78613.1"
                     /translation="MNWKRMWIVARTDLKQLAQAKDYWVPMVALGALFFLVVPAILLL
                     TITKIGNVEIVNQISNTLKVLPKAAQSQIRGTSPEGKTSFALAVFLFAPVAVVVPLTI
                     STAVGAATIVGERERGTGEFLAHSPASAREIYLGKLIASLVPGYLTTIVGFGLYSVLV
                     NVIAGPEVGGWFFPTAQWWILMFWVIPPFLAFTLSLVLRLSARVKSTAAAQQASGLVS
                     LPLIMIAYGQSSGALFGAQVSGFYVGLVAWVIAIVSLSRGVRSVTRARLLGVANEV"
     gene            complement(15580..16110)
                     /locus_tag="PKY13_01445"
     CDS             complement(15580..16110)
                     /locus_tag="PKY13_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT78614.1"
                     /translation="MTSQPGGEAAPALDGGPDDPGNPPVLRVGDRAPVFELDGIDGST
                     GERRHFTSGEFAGAPLIVAFYPADNTPVCTEQLLSYTANIGLLDRDGIQLVAISPQDP
                     ESHQLFAKAHGGFAFPMLSDVDKSVGRAFGILGLLDLYRRSTFVIDADGVVVGAHRSI
                     GPGSGFRPLQELVAEA"
     gene            complement(16103..16852)
                     /locus_tag="PKY13_01450"
     CDS             complement(16103..16852)
                     /locus_tag="PKY13_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT78615.1"
                     /translation="MSGHSKWATIKHKKGATDKARGKQFAKLIKQVEVAARAGGGDRE
                     SNPTLRTMFQKARAASVPLDTIERAVKRGTGELEGVEYQEVNYEGYAPGGVAVYVEVL
                     TDNRNRTGPEMRSVFSKNGGSIAEPGAVAWQFERKGIVTVDGAVDEDELMEAALEAGA
                     EDISRDAENNWHVVADGSATHEVSSALEAAGYAVMSAELTMIPSSLITIDSLETAKAI
                     LKVIEALDDHDDVQEVYANFDIPDALMAELD"
     gene            complement(16867..17448)
                     /gene="pdxT"
                     /locus_tag="PKY13_01455"
     CDS             complement(16867..17448)
                     /gene="pdxT"
                     /locus_tag="PKY13_01455"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOT78616.1"
                     /translation="MTPHVGVLALQGAFAAHQRMLEGLGASTIAVRQPKHLDGVDALV
                     IPGGESTTMSKLAVSTGLFQPISERLAAGMPAFGTCAGAIMLASHIADGRADQRDFET
                     LDIDVRRNGYGRQIDSFETEIDVDGLDAPFHATFIRAPIIERTGDGVQVLAAVGDNAV
                     FVRAGAVMATTFHPELGADDRLHRLFLDAITSS"
     gene            complement(17525..18433)
                     /gene="pdxS"
                     /locus_tag="PKY13_01460"
     CDS             complement(17525..18433)
                     /gene="pdxS"
                     /locus_tag="PKY13_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOT78617.1"
                     /translation="MTDSASPVAPSSLRGTVQVKRGLAEMLKGGVIMDVVDAAQARVA
                     EDAGACAVMALERVPSDIRRDGGVARMSDPQMIEEIKAAVTIPVMAKARIGHFAEAQI
                     LQALGVDYVDESEVLTPADETHHIDKWAFDVPFVCGATNLGEALRRISEGACMIRSKG
                     EAGTGNIVEAVRHLRQILGDIRKITQADPAELFEWAKQLQAPLGLVQEIHDTGVLPVP
                     MFCAGGIATPADAALVMQLGAQAVFVGSGIFKSEHPDIMAKAVVEATTNFDDADILVK
                     VSRGLGKSMAGLEIGGLETTFADRGW"
     gene            complement(18527..19621)
                     /locus_tag="PKY13_01465"
     CDS             complement(18527..19621)
                     /locus_tag="PKY13_01465"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOT78618.1"
                     /translation="MSRSPRNLTLRSTACCAVALLMATSCGGDDKKADPPATTTTSTS
                     TTTTEATTTTTAVPVAAATAPLTGLPIDDATAVKLLRPALAVKIDNSVDAMPQEGLNQ
                     ADIVFEIKVEGISRLMAVFHSQDAGEIGPTRSARYSDPPILALLGKPLFGWSGANEGV
                     VRDVYRSEWVVNVNWDKHPKDYFRKSGRKAPHNLFTSTDKLFAYAEPNQGPPLTLFQY
                     LGEGETNAGAIPMPGMTEKVGDTPSAWVWDPAGNQWLRWQYNKRDQTEGTGQANAANV
                     VIMEIQYRGGSKTPTAKTEGTGKVTVLGAGGTAVTGTWNRPSQLEPITLTGADGQPIR
                     LTPGRTWVEFTDGATAAPMSPEVAASMLAG"
     gene            complement(19655..20515)
                     /locus_tag="PKY13_01470"
     CDS             complement(19655..20515)
                     /locus_tag="PKY13_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78619.1"
                     /translation="MSIEARTLLDRTDRNREATKFLVVPQALATAIVVGVIVGLVATW
                     WIGLVAGVLSGVIVAVILPKSASAGLATVFGARPAPEPEFPRYHNLMEGLSISSGSVM
                     PTLQVIDEARPNLAVYGPPDEGVVIATTGLLEQLDRVELEGVLAEALVRLRSHDAELG
                     AQAAMLVCGPLVRNGPRRPGRPMGYVAFLAGWRAGRLRRVLGDQREFLSDLGAIDMTR
                     YPPGLGSALEKMEALGTGVVSATWGTAHLWLADPLVEAADTDTTAYQLSALFGGGAPI
                     DQRASLMAEL"
     gene            complement(20541..21101)
                     /locus_tag="PKY13_01475"
     CDS             complement(20541..21101)
                     /locus_tag="PKY13_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOT78620.1"
                     /translation="MIWVLVILIALVVIVGLAFMFTYNGLVGLRNKIEAAYAQIDVQL
                     NRRHDLIPNLVATVKGYAAHESGTLEAVIQARNMAMSANSIEDKAAAENVITGALKSV
                     FALSEAYPDLKANTNFQQLQTELSSTEDRIAYARQYYNDNVRAYNTKIQSFPSVVVAN
                     MFKFEERQYFQADDQSRGNVAVDFGA"
     gene            21432..22526
                     /locus_tag="PKY13_01480"
     CDS             21432..22526
                     /locus_tag="PKY13_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT78621.1"
                     /translation="MLRNDGPRVIEAERPATQTNVRTELARIGRQWLVSITRLVELSA
                     ELDQSGEWALDGSPTCAHWIASVLDIEVSTAREWLRVGRELRRLPKTRSALRDGRLSY
                     TKVRSVTRLATAEHEGELLDIAERTAPGQLGTALAAWSARNEDEATRNQRHQRDRSLT
                     WRTEPDGMVTASIRMTPEQAALVIAAIDAEVMRGDGRETDPNTDKNATDGEIDASGTR
                     PSHVRWSSLGQQRVDSLVRLIGGGGSEVTTEVILHVRADGCTLHDGTPIGGSIVERIA
                     SEASLRVMIHDAESRPINVSGTHRHHTKRQKLVVKERDDVCVDCGGTDFLEYDHVPDF
                     AVTKHTLVEETDLRCSRCHRARHSTAGGGR"
     gene            complement(22540..23667)
                     /locus_tag="PKY13_01485"
     CDS             complement(22540..23667)
                     /locus_tag="PKY13_01485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT78622.1"
                     /translation="MHIGMVCPYSLTVPGGVQGQVLGLARALRERGHNVQILAPADAP
                     PTEGGVTVIGPSVMNAANGSIAPISPNPQAQLRTMRALWDEKFDVLHLHEPLVPGPTV
                     TALMLRSAPIVGTFHAAGEQPMYKSMSSLARWFGSRIDAKVAVSQDAQALAGVAIPEP
                     WTILFNGVEVDQFASAEPWVDPPGPDGAVDDRPTVLFVGRHEERKGLGVLLDAVGLID
                     QDLRVWVAGSGPETEELQARHAADHRIEWLGRISNDERNRRMAAASVFVAPSLGGESF
                     GVILLEAMAAGAPVVASSISGYVGVAGPLDGEPAAALLPERGDPAAFAMAISEVLGDD
                     ALADRLRASGRDRANRFSMDRLAEAYTVLYEAVIAEAALNA"
     gene            complement(23682..24680)
                     /locus_tag="PKY13_01490"
     CDS             complement(23682..24680)
                     /locus_tag="PKY13_01490"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HOT78623.1"
                     /translation="MAPLDPIGAARAAARKALAPALERAAGPAIVGAYKLGEALAQRA
                     PRQIGAPVLAAAGHAAALRGGDKRLIVERNLRRVYGRDLTRNEINTKVQATFESYARY
                     YYDSFRLTGMSVDEVAAGFTVEGIEHIEEAMEHDAVGPVLALPHLGGWEWAAFWITQV
                     RNWGLAAVVEQLEPPELFEWFLEFRTSLGMNIIPLSPTAADEVAMAAANKEIVCLLCD
                     RDLTRAGPEITFFGETTSLPAGPAVMALRGGCRVLPTAVYFTERGVHGVVRPPIEIER
                     TPGATSIREDVVRFTQVLATELEGLIARAPEQWHLLQPNWPSDYEALGTTPPPRRG"
     gene            complement(24684..25400)
                     /locus_tag="PKY13_01495"
     CDS             complement(24684..25400)
                     /locus_tag="PKY13_01495"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT78624.1"
                     /translation="MLDGRFRGQIETWVKPVGMSVKRAGIPADAITAVGLAMSVACAI
                     AVGAGALRLGVILLALTGIPDTLDGAVAKASGTSSQRGAFFDSVSDRVTDALLFGGVA
                     WYLASQTDQSRYLPVLAFAAFGTAVLPSYIRAKADALGLDARGGLVERAERFVILGAG
                     LLFDRFLIAALVVLVVLNMATAIQRFVKVWNQASKPLPMPRPAAARRRSRASTPSPAA
                     ARWQARRAEARERARTRRGY"
     gene            complement(25558..26082)
                     /locus_tag="PKY13_01500"
     CDS             complement(25558..26082)
                     /locus_tag="PKY13_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78625.1"
                     /translation="MTESPESTPEIDDADVVDELPADLNVAEASADYLLPNNNRRRIP
                     AAIYLLISAVCLLLWITKRDSSPLVNDGYLWVAVFLGGFAIYGLIAGRTLLVDETEAL
                     ATANRTVGFTVGHASAQMVWRGWLSRPVWRLLAYSADNPPSRRAIVVVDGISGEVVEW
                     FAEQNPEDWATSAS"
     gene            complement(26206..26712)
                     /locus_tag="PKY13_01505"
     CDS             complement(26206..26712)
                     /locus_tag="PKY13_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOT78626.1"
                     /translation="MPLDHLWATWRSNYVTSSVESRSDAPTDGRTLFERILAADGTDD
                     EKHLLHRGRTCFVLLNKFPYTSGHLLVLPNRGVADLEALTDDEHIELWALVRSSVVAL
                     KAAFRCDAVNVGVNLGAAAGGSQSDHLHVHCVPRWTGDANFIGVAAETRVLPVSLDDA
                     LASLRAVW"
     gene            complement(26729..28690)
                     /gene="thrS"
                     /locus_tag="PKY13_01510"
     CDS             complement(26729..28690)
                     /gene="thrS"
                     /locus_tag="PKY13_01510"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT78627.1"
                     /translation="MSDITITLPDGSERSLPEGATGTTLAESIGPGLAKAALVVDVDG
                     EPHDLAAPLPDHATVKFVTDRDPEALEHLRHSTAHVLAQAVLEMWPGATFAGGPAIDD
                     GFYYDFELPEGATFSEDDLAAIDARMREIIAADQTFERFELPVPEAKELMAAHPYKQQ
                     FMDLAASGDDADGEVSAGERISFYRNTPAFVDMCRGPHVPSTGRLGHFKLMRVAGAYF
                     RGSEKNPMLQRIYGTAWANKKDLAAHLHRLEEAIKRDHRRLAHDLDLVSWPEELGPGL
                     AVWHPKGALVRKTMEDYSRFRHEHGGYDFVFSPHIAKSVLWETSGHLDFYADSMYPPM
                     EMDGATYYPKPMNCPFHVMVYRSQQRSYRDLPLRLFELGTVYRYELSGAVHGLLRSRG
                     FTQDDSHIFCTLEQVEEELASLLDFVLDVLRSFGFTDFQAKLSTRPLEKSVGDDKLWE
                     LATNGLRAALDAHGLDYIVDDGGGAFYGPKIDVDVRDAIGRSWQLSTLQLDFNLPERF
                     GIEYIAPDGTRQQPVMIHRALFGSVERFFGVLLEHYAGALPTWLAPVQVRVLPVADAH
                     ADYASEVLSSLKAVGLRADIVWADESLGNRIRKAKGEKLPYVLVVGDDDVANGTVGVN
                     ARGNDVERDVAVAAFTERVTAEVADFRGV"
BASE COUNT         4579 a         9708 c        10095 g         4467 t
ORIGIN      
        1 ctgggcgtcg ttggcgccgt gacccaggct gaacgccgcc gccgagagga gctgaccgcg
       61 tcggaacccc cggttgaggc cgtcgacgtt cttcacgcgg tagcaacacc acaagatgat
      121 cagcgagatg atgagcccca acgccaggcc gatgagcggc gacaggatga tgaacgctgc
      181 gatcttgcgc atgccgtcgg ccaccagcac gtcgaagccg ccccgggcga ccgcagctcc
      241 ggccatgcca ccgacgagcg cgtgggagga cgacgacggc aacccgaggt agaaggtcgc
      301 caggttccat ccgatcgcac cgaggagccc ggcgaagatc gcaccgacgg tgaggacgtt
      361 cgacagcggg tcgaccacgt ccttggcaat ggtgttcgca acctcggtgc cgaagacggc
      421 gaacgcgatg aagttgaaga acgccgccca gaacacggcc agctgcggcg acaggacccg
      481 cgtcgacacc acggtcgcga cggagttggc ggcgtcgtgg aaaccgttcg tgtagtcgaa
      541 cgccagggcg acggcgatcg tgaggacaac accgaacgtc acgacaactc cccggtgggc
      601 gcgatcacgc gttcttgacg atgacggact cgacgacgtc gctgacgtcc tcgaggcggt
      661 cgatggcgtt ctcggtggcg gagatgatgt ccttccactt caagaccgtc atcgtgtcgt
      721 gctcggcgaa caaccgcgcg atggcacgac ggtagatgcg gtcgccctcg ctctcgagct
      781 gacccatctc catgaggagc gagtcgaggt ccttcatgga ctcgaaccgt tcgaacaggc
      841 tcacgttggc ggccgacata cggacgagca cgtcgcacag ctcggccatc tccggcatgg
      901 gctcgcggat cttgagcatg tgcaggaggt cggcgacatg gaacatgtcg tcggtcacgt
      961 cgtcgagccg ctctgcgatg ttgtggatgt cttcccggtc gaacggcgtc acgaagctcg
     1021 tgtggagccg ggccagcacg tcacgggtga ggtcgtcgcc cttgcgctcg gccgccagga
     1081 tcgcgtcgcg ctgcgctccc gagtcgacga ggttctcgac catcttctgc agcagcagcg
     1141 cacagttgtg gacgttgtgc gccgtgtcga tgaacaggtc gaagaacttg tcatcggtcg
     1201 ggacgagccg gaatcgcatg ggatcttgat gctccgaacg aggcagcttc ggcgtagccg
     1261 aatcggtctg gggtggttgg tcgggccgac cgaagattgc ctcacagtga acacgaggca
     1321 acgaacgggt gaacagtagc cgccccgagg gagcccagta ccaaccgatc cggagggtca
     1381 gttgccgccg ccgggcagca cccggtaggc gtcgtacacc gagtcgatgc ggcgcagccc
     1441 gctcaggatc gcttccaggt gcgacgggtc gcccagctcg aactcgaagc gcatcttcga
     1501 cacccgatcg gggccggtgc tcgtgaggca ggacacgatg ttgacctgct ggtcggacaa
     1561 catgtgggtg acgtcctgca acaggcggga gcggtcgagc gccttgagtt cgaccaccac
     1621 gctgaacgtg ccggtcgagt ggtcgtccca cgtgacgtcg atgaggcgct cgccgtggcc
     1681 gcgggccagc gacgctccga tctcgcagtc ggtgcggtgg atcgacacgc cgcggccccg
     1741 cgtcatgaac ccgatgatgg gatcacccgg caccggcgtg cagcaccggg cgatacgcac
     1801 cagctcgttg acctggccct cgacggtgac cccgaccgag cccgtgccgg tgcgacggtt
     1861 gcgggcactg ccgctcgaga tgacctcgga gcggccgttg tcggcgccac gcatctggct
     1921 gacgaccttc gcgaccacgt cggacgcctc gatccgatgc tcgccgatcg cggtgtgcag
     1981 ggcatccaac gagggcgtgt ggaactcgcc ggcgatctcg gccagcaact cgtcggtcgc
     2041 cagcttctgc acgtgggacg actcgctgcg cagcgccttg gccaacaact cccggcccga
     2101 ctccagcgcg tcctcgcgac gctcgcggct gaaccactgc ttgatcttct gctgggccga
     2161 ccgtgtggcc acgaagtcga gccaatcgag cgacggaccg gcaccctcga ccttggacgt
     2221 gaagatctcg accgtgtcgc ccgacgtcag gtgatgctcg agcgggacca ggcggccgcc
     2281 gacgcgagca ccgatgcagc ggtggccgac ctcggtgtgg atggcgtacg cgaagtcgac
     2341 cggcgtcgca ccgagcggca ggttgtgcac gtcgcccttc ggcgtgaaca cgtacacctc
     2401 gtcctggtcg aggtcgctct tcaggttggc catgaactcg gccgggtccg acatctcgtg
     2461 ttgccagtcg accatgcggt tgagccagga gaggtcctcg actcccccgc caccatcgcc
     2521 ggccttgtac gagaagtggg cggcgatgcc cgactcggcc cgctggtgca tctcacgggt
     2581 acggatctgg acctcgaccg ccttggcctg cgggccgatc acggtcgtgt gcagcgactg
     2641 gtacaggttg aacttgggcg tggcgatgta gtccttgaac cgccccgcca ccggcgtcca
     2701 gatcgagtgg atcgacccca gcgcggcgta gcagtccttc tccgaggcga cgatgacccg
     2761 catgccgacc aggtcgtgga tgtcgtcgaa ctccttgccc cgcacgacca tcttctcgta
     2821 gatgctccac atgtgcttgg gccgcccggt gacctcggcc tggatctgca cctcggcgag
     2881 gcgggagcgg acctcctcct cgacctgggt gaggtacagc tcgcgctcgg gcgaccgggt
     2941 cgcgaccatg tgatcgatct cggcgtagcg cttggggtgg agcgccgcga aacacagatc
     3001 ttcgagctgc tgcttgagct cgcccatacc gagccggtgc gccagcgggg cgtagacgtc
     3061 catcgtctcc cacgcggtct tctcctgctt gtccgccggg agcgcggcga tggtgcgcat
     3121 gttgtgcagg cggtcgcaga gcttgatgac gagcacccgg atgtcgtcgg ccatggccac
     3181 gagcatcttg cggatggcgg ccgcttgctg ggcctccttc gagtcgaagt ggatgcgatc
     3241 gagcttggtc acaccgtcga ccaaagccgc gacctcggcg ccgaactcct tctcgatgtc
     3301 cgccagcgtg aagtcggtgt cctcggcgac gtcgtggagg agggccgcca cgatcgtcgt
     3361 ctcgtccaga ccgaaatgag ccacgatcgt ggcgactgcc aacgggtggg tgatgtacgg
     3421 ctcgcccgaa cgccgcatct gcccctcgtg cgccgcctcg gcgacctcgt aggcccgcgt
     3481 gatcagctcg gaggacgcct tgggatgcgc gttgcggaac tgctcgacca ggggcccgac
     3541 cttctctgcc gccggcagct ggtggcgacg ccacgggagg acacgggtca cggcgggcat
     3601 cgggcggcgg gactcctagg cgtaggtgat gacggtgtgc acggggaggt cgcccagccg
     3661 cgaccggccg tcgagcgggg cgatctcgat gaggaacgat gcggcgacga catcggcgcc
     3721 catcgaggtg agcaacgcca ccgtcgccgc cgctgttccc cccgttgcga gaacgtcgtc
     3781 gacgacgagc acccgctggc ccgcagcgac ggcgtcggtg tggatctcga gggagtcggt
     3841 gccgtactcg agctcgtagg tctccgtcac cgtggtgtgc ggcaacttgc ccggtttgcg
     3901 gaccggcacg aacccgactc ccagccggtg ggcgacgggg gcggcgaaca tgaacccgcg
     3961 ggcctcgacg ccggcgacca ggtcgacctg cccgagatgc ggttcggcga gcgctccgat
     4021 cgacgccgag aaggcggcag ggtcgcccag tagcggggtg atatcacgga acatcacccc
     4081 cggctggggg taatccggga tgtcccggat gtggtgtcgg agatcggcgg gatccatcgc
     4141 gtcacgctac ctgggtggga gcttcggcag gaccgcccat cagcgccgtt tgccctgctt
     4201 gcggggccgg ggcgggtgtt cccgctggta ttgcgccgcc ttggtcgcca gcggggagac
     4261 cttgggggcg gtggacggtg cgctgtcgcc gtcgaacgcg acgtagggcg acgacgagac
     4321 cggcgagccg gcgagggcca cctcggccac cgggatccag tcggcctcga cctcggcgcc
     4381 gcggtcacgg gcccgctggc ggatcttcgc gtagcggggc tcgcgctcct tgagccacac
     4441 gacgagcggc gacgcgatcg cgatcgacga ataggcgccg gccacgaggc ccaccaacag
     4501 ggcgagggag aagtcgagca tcgtggcctg gccgtagacg atgccgccga tgacgaggat
     4561 cgcgacgacc gggaggatcg cgacgaaggt cgtgttgagc gagcgcatca gcacctggtt
     4621 gagcgaacgc cgcatgatcg cgttgtacgt gaaccggccg gtgcggtcgt accgcgccgc
     4681 gttctcctgc acccggtcgt agacgacgat ggtgtcgtac aacgagaagc cgagaatcgt
     4741 cagcacggag atcaccgtgg ccggggtcac ctcgaacccg aacaccgagt agaagccgag
     4801 cgtgaggatc acgtcgtgca ccaccgccag gagggcggac acggccatac gccattccag
     4861 ctgccaggac atgtacgccg cgatgacgat gaagaacacc acgagcgccc gcgccgcgtc
     4921 cttggtgagg ctggcgcccc acgacgggcc caccgtgttg gtggcgatct cggtgacctt
     4981 gatgccggcc ttctcggcca gcgcgtcggc gatcttgtcg ctctccgcga tgttcttcgc
     5041 ggcctcggcc tggacccgca cgatgcggcg accgttcgcg tcggtcacgc tctggatctt
     5101 cgcgccgttc tccttgtcga aggaggcgag gacgtcctcg gcgtcgccgg tcgacatggt
     5161 cttcgacggg acctcccaca cggcaccgcc gcggaagtcg atcgacaggt tcagccaccg
     5221 ggtggccagg gatccgacgc cgatgaggac caggatcgac gagatcacga tcatgatccg
     5281 ccacaccttg gtgaagttga agtcggtctg gacgcggtag agacggctca gggtgcccat
     5341 cagcgctcac ccccttcgtt cgtggggtcg tcatcggtgg cgaccacctc cccggcgtcc
     5401 ggctcgtacg catccggctc ggcgtcggtc gcagcctcga cggcttcgat ctcggactcc
     5461 ggagcaacct ccggctccgg ctccggctcg ggctcaacct cgggctcgtc ggcgacgatg
     5521 gcgcccgtcg cgacgggctt ggaccccgcg gcggcacgag tggatgcgtc ggtcctcgcc
     5581 gagcgaccgg tcgcaccgcc tgcccctgcc ggggtcgttg ccggcatcgg gatgccgaac
     5641 agtgccggac gcaggccgag cttcgaccgg gccgcgagca cggtcgccgg gcggatgaac
     5701 atgtacgcga gcacgaggtc gatcaccgtc gacagggcca ggtagaaggc gaagcccttc
     5761 accggaccga cggagagcac gtagaggatg atggcgccga tcagcgacga cacgtcggcc
     5821 ttgatgatcg tgccccaggc ctggtcgaag ctccggtcga cggccgagcg cagcgtgcgg
     5881 ccgtgcatca cgtcttcctt gaggttctcg aagtagacca cgctcgagtc gagcgacaca
     5941 ccgatcgaca cgatgatgcc cacgatgccg gcgagggaca gggccaggcc gctgtagtga
     6001 ccgagcaggc cgaccaccgc ccagagcaac gctgcggtca ggatgagcga acccacggtg
     6061 atggcgccga ggagtcggta gtaggcgatc acgtacaaca cgaccacgcc gaggccgatg
     6121 aggccggcga tgatgcccga tcgcagcgca ccggaaccga gcgtggccga cacggtctgg
     6181 acggtctgcg gctcgagctc gagaggcagc gagccgtagc gcagcgccag ggcgaggtcc
     6241 ttcgagctct gctcggtgaa gctgcccgag atctggatct ggtcacggct gaacgatgcg
     6301 gcgttgatgg cgggagcact gatgacctcg ccgtcgagca ccaccgcaag ctggccgacc
     6361 tgggccccgc cttcgccgga ggcgatgccc gggcactcgg gggcaccggc gttgcacagc
     6421 gccgcggcct tgttgaacag gtcgatgccg tccttgccgt ccttgtagac cgggttgacg
     6481 acccacttgc cgttggcgag cccggcgctg gccgtctcga tggcccgccc cgtgaggagc
     6541 gtcgggccga gcttgtactt cacccgggtg ccgtcggagt cgggctggcc caggagcaca
     6601 acctcggcgt cggccttgtc gtcggcgggt gccgtcgcct tctcggcgtc gttgttgacg
     6661 gcctgatcca gctggtagag cttggcgaag ctgggatcgg tgacgtcgat gccccactgg
     6721 ttcttcggca cgtagacggt cgtggtcgtg acggtgccgg gcgcggtcgt cgtggtcgcc
     6781 gtcggcgccg ccgttgtggt ggttgctgcc tcggcggccg tggtggtggt cgactggcga
     6841 cgggcggcag cgcccttcat cgaactcttg ccaccgctgc ccgcaccgct cgcggcggtg
     6901 gtggtgggcg ccgcggtcgt cggcgccacg gtcgtcgcag cgggatcgac ggcagtcgtc
     6961 gcggtcggat cggtcggcac ggtcgtcgcg gtcggatcgg tgaccgtcgg atcggcagcg
     7021 gtcgccgcgg cgttctcctc gtcgacgatc tgttggacgg tcacgccctc ggggatcttc
     7081 agctcctcgc gcagcgcggc gatctgctcg tcggcgtcct tcggcggcgc ggccgtcggg
     7141 gccgccaaca ccgggcggaa ccgcagctcg gccgtcttgc cgacgaggtc gaggacccgc
     7201 ttctggtcct tgatgcccgg cagctggacg acgatcgtcg tgccctggat cgtgatgtcg
     7261 ggttcaccga cgccgatcgc gttcacgcgg gtctcgatga cccgctgtgt ctgctcgagt
     7321 gcttcctggc tgaccttggt ctgcttcttc ccggccttga ccggctggag gacgaccgag
     7381 acaccaccct ggaggtcgag acccaggttg ggggtgaagt cggccaggat cacggcgagg
     7441 caggagccga gcgacagcac cgtgaccagg gcgagcggga cgagtccctt tttcttcttc
     7501 atgaggagtg gcgtttccct gtcgatcgtt caaggtcgga atgacgattc gcaaccggcg
     7561 gagcatacgg gcttcgccgt gccgacgtca ggtatgggcc tcggcccaca catcgtactg
     7621 aacgtcattc ggccggagtc gtgaccatgt gagcgcgagt ccggcgacga gggcggtgct
     7681 ctcgtcagca agcgctcgtc gatgcgggcg ctggtggcgg acgtcgatcg ggccgaggtc
     7741 ggcccgatcg ctcaggactt cttcgacttc gacgacgggg cgtcgtcgct gccagcgtcg
     7801 gtctcgccgg cgacctcgtc gaggtcgatg acctcgtcgg agctggcagc cgcgccaccg
     7861 agtcggttga ccgacgtctt ggacacccgg atcaccacgt cggtgtcgac caccaggtgg
     7921 gcgatgttgt cctcgaggta gctgatcgtg ccgtagatgc ccgacgtcgt catgacctcg
     7981 tcgccgacct tgagcgaact catcatctcg gcctgcttct tcttctgctt gcgaggcgac
     8041 acgatgagca ggtagcccat cagcgcgatc agcgggatga agatgatgaa gctgccaccg
     8101 gactgttgtt ccgcggcgat gatggacaga agggcgttca cgagtcgcgg agcctagtgc
     8161 gccacggggc tcaaccccag accgcggcca cctcggcgcg cagcgcgtcg agccggcctg
     8221 cggcgatcgc ctcacggatg cggtccatga gccgcagcag ccaggccgtg ttgtgcagag
     8281 tgcagagcgt ggcgccggtc ggctcgccga ccgtcatgag gtgtcgcaga tagcccttgg
     8341 agtaccggcg gcacacctcg caggggcacg acggatcgat cggctcgtcg ctgcgctgga
     8401 actccttgcg acggatgctc acccggccgc cgtcgctcag caacgtgccg tggcgggcga
     8461 ggcgggtcgg cagcacgcag tccatcatgt cgatccctcg cgccaccgcc tccacgatcc
     8521 cgacgggatc gcccagaccc atgaaatagc gcggctggtc ggcgggcaga tgggcgacgg
     8581 cggcctcgag cgccggcaac atcacgtcgc gggtctcccc caccgacaac ccaccgacgg
     8641 cgtacccgtc gaagtcgagc tcgacggtgc gctgcgccga ctcggcgcgt agatccgcat
     8701 cggtgccgcc ctggacgatg ccgaacagcg cctgccgctc gacgatcgac tcgtcgccat
     8761 gacgaccgtg cgacgcccgg gctcgtgccg cccagtcggc ggttcgctcg acggcccgtc
     8821 ggagctggcc gggcttggcg ggcaacgccg tacacacgtc gagcaccatc tggatgtcgg
     8881 cgccgaggag ctcctggatg cgcaccgctt cctcgggggt cacccggtgg gtcgacccgt
     8941 cgtaggtcga tttgaaggtg gcgccgtcgt cgtcgacctt cgggttgagc gagaagatct
     9001 ggaacccacc ggagtcggtg agcatgtgcc ccgaccagtc ctcgaaccgg tggagcccgc
     9061 ccaggtcggc gacgacgtcg gcaccgggcc gcagcatcag gtgataggtg ttgcccagga
     9121 tgatctcggc gccgaggcgt tcgacgtccg cggtcgtcac cgtgcggacc gcgccgcgag
     9181 tgcccaccgg catgaacacg ggggtgcgga actcgccgcg cgccgttcgg acccttcccg
     9241 tccgagcggc cccgtcggtc gcctcgatgt cgatgtgcaa cttcatgttg atgcgctttc
     9301 cgtcgaccgc tcccgctcca gcaacatggc gtcaccgaac gacaggaacc ggtagccgcg
     9361 gtcgagcgct gcagcgtaga tgtcgcgcca gcgtggcccg acgaacgcgt cgatcatcac
     9421 gagcagcgtg gaccggggca ggtggaagtt cgtcatcagc acgtccacga cctgccagtc
     9481 gtaggggcgg cggatgaaca ggtcggtgct gccggcgagc tcgccggtcg ccgccgccga
     9541 ctccaacgct cgcacgacgg tcgtcccgac cgccacgatg cgccggctct cggaacgggc
     9601 acgctcgatc gcgtcccagc tcgtcggagg gacccggtag gactcgtggt gcatcacgtg
     9661 gtcctcgacg tcgtcggtcg tcatcgggcg gaaggtgccg agcccgacca cgagttcgac
     9721 ccgggcgatc tgtacaccct tcgcccggat ccgatcgagc agcgcgtcgg tgaggtggag
     9781 gcccgccgtg ggtgcagccg ccgaggcggg acggttggcg aacaccgtct ggtaccggtc
     9841 ggggtcgtcg aggtgagcgt cacccaggta cggcggcagg ggcatctcgc ccacccgctc
     9901 gagagcgtcg agcagggtcg aacccgccgc aggggctccg tcgacgaggg ggcacacgat
     9961 ccgacggcct tcaccgaggt cggcgccgaa ctcgagcgag aaccggtcgt cgactgcgac
    10021 caccgttccg ggcggcagct tcgccgaggg ccggacgagt gcgtcccacc agccgtcctc
    10081 gatggcggtc aggaacaaca cctcgccgga accgccggtg gaacgggtga agcgcacccg
    10141 cgccgggagc acccgggtgt cgttgactac gaacacgtcg cccgcccgca ggtgatcggg
    10201 aagatcgccg accacggcgt cgacgagacc ctcggcggtt ccgtcgccgc gtccggcggt
    10261 gtcgacgagg aggcgcgccg agtcacgggg ctccataccc acggtcgcga tggcgcgttc
    10321 gggtagctcg tagtcgaact cctgggcctt catcggaggt tcgtcagccg agttcggcca
    10381 ggcgcgggac caggatctcg ccgatggcgt gcaccgtggg cacggggtcg gccgtgctcg
    10441 gcgagaggaa cacctcgtcg accccgagcg ccgcgtactc cgccatctgg gcaacgaact
    10501 cgtcgacggc cgtcggatcg gagcagtccg ggtgcatcgc catcatcgtc aggcggatcg
    10561 agtcgacgtc ggtgccctcg gtctcgcagt gcttcgcgag cgtggccttc aggccctgga
    10621 aggcgccggt gccgatcggg aaccagttga ccgcctgggc gtagcgggcg gcgaggcgca
    10681 gcgtcttgtt ggggcccatg ccgccgatca tcaccggagg ccggccactc agcggctggg
    10741 gcgagttgag ggtgcgggcg agctggtagt gcttgccctc gaacggtccc tcgtcgtcgc
    10801 tgcacatctg gtcgtagatg gcgagggtct cgtcgaggcg ctcgaaccgc tccgcggtcg
    10861 acgggtacgg cacgccgagc gcgacgtgct cctcctcgaa ccaggcggcg ccgatgccga
    10921 gcgtcgcccg gccacccgac aggacgtcga gcgttgtcac ggtcttggcc aacaggccgg
    10981 ggtggcggta ggtcacgccg gtcacgagga gctgcagatt gatccgcttg gtgaggccgg
    11041 ccgcgaacgc cagcaccgag tagccctcga gcatggcgtt ctcgggtggg ccggcgatct
    11101 cgatctggaa ccagtggtcc atcgccgtca gcgagctgaa cccggcctcg tccgcgatct
    11161 gggcgacctc ggcgatcttc ggaccgatgg cggcagtgcc gccttcccaa gtgaagttgg
    11221 cgagtgaaca ggcggtcttc atgcgccgga gcctacgcgg cgcctgcgtc gagggttttg
    11281 tgctgcgagg agcgacgtca gcgtcgctcc tcgcagcaca aaagccccgg cgatcgggtc
    11341 agtcgaagag gctggggtcg gcggcggaag cgctcgccga cggaggcggc acgaggccga
    11401 tgtgtcgcca ggcggccggc gtcgcaaccc ggccacgggg cgtccgcatg agcaggccct
    11461 gctggatgag gaacggctcg tacacgtcct cgaccgtctc ggtgggctcg gccacggcga
    11521 tggccagcgt gctgagcccg accggaccac caccgaacag gtcgcacagc gcggtgagga
    11581 cggcgcggtc gaccttgtcg aggccgagct cgtcgacgcc gaacacggcc aggccgtcgg
    11641 aggcgacctc gcggtcgatg cgggtgtcgg cctcgacctc ggcgtagtca cgcacccggc
    11701 gcaacagacg gttggcgatg cgaggagtgc cgcggctgcg ccgggcgatc tcatgggcgc
    11761 ctccggcatc gatgtcggcg gcgacgatgc cggccgcacg cctgacgatc gactcgaggt
    11821 cggcgacgtc gtagaactcg aggcgagcca cgaggccgaa gcggtcacga agcgggccgg
    11881 tgatcagacc cgtgcgggtg gtcgctccga cgagggtgaa ccggggcagg tcgaggcgga
    11941 tcgaacgggc tgcgggcccc ttgcccaaca cgatgtcgag ctggaagtcc tccaccgccg
    12001 ggtacaacac ctcttcgacg gtgcggctca gccggtggat ctcgtcgatg aacaacacgt
    12061 cgccctcacc aagcttggtg aggatcgcgg caaggtcgcc ggcgcgttcg agcgtgggac
    12121 ccgaggtgac gtgcaggtgc acccccatct cgttggcgac gatgccggcg agggtggtct
    12181 tgccgagacc gggcggaccg gcgaacaaga ggtgatcggc cgcttggccg cggcggcgcg
    12241 ccgcttcgag gatgatgcgc agacggtgct tgagctcggc ctgaccgacg aactcgtcga
    12301 gatcacgggg ccgaaggttg gcggcgtcga tcgccgcctg accgtcgacg tcgcgcacgt
    12361 cgaggccgtc ggcgaccacc gcgtcgagcg agttgaactc gggatcgagc agttcgtctc
    12421 tcacacgagc cccctcacgc gaccgcgagc ttctgtaggg cctgtcgcaa caggtcggag
    12481 gcgtcgccgt cgagcggcag ctggccgacg acaccggcga tctcctcgtt gccgtagccg
    12541 aggccgacca gggcctcgcg aacgtcggcg tgggccgagg ggccggtggc agccgacggc
    12601 gacgcaccgt cggcggcgag cgcccggagg tcgacgtcgc ccgacccgtc gaggcgtgac
    12661 ttcagctcga cgagcaggcg ttgcgccgtc ttgcgcccga cgcccggcac caggcacagc
    12721 gccgacacat cgtcggtcgc cagggcgagg cggagcgccc gcggcgagtg cgtcgacaag
    12781 atcgcgaggc cgagggccgg gccgacgccg tgcgtgccga tgagcgtctc gaacacgtca
    12841 cgttcctcgc gaccggcgaa cccgtacagg gtctgcgcgt cctcacggat gtgatggtgc
    12901 acccacacga agacctcgga gccctcgaca ccgagcgcac cgctcgtggc cggggtggtc
    12961 gacatccggt agcccatgcc gccgacctcg acggtgacat cggtaccggt gcgatcgagg
    13021 agcgtgcccc tcagtgaccc gatcatcgga gccccctcat cggacggtca ccatggacgg
    13081 ggcgtaggcg acgtggcaga gggccacggc cgcggcatca gcggcatcgg cgggacgcag
    13141 cacggcgctg tgacccaaca gtcggcgaac catctcctgc atctgctcct tgtcggcgga
    13201 gccgtagccg gccaccgctt ccttgacctg gttgggggaa tactcgatca cgtcgcaccc
    13261 gcggcccgcc gcctcggcca tcacgatgcc cgaggcctgg ccgaccgaca tcgccgtgct
    13321 cacgttcacc tggaacaaca cccgctcgat ggcgacggcg ctcggcgggt agtcgtcgag
    13381 gagctcgcgc acatcgcgct gcaggatcgc gaggcgtttg ggggtgctga ggtcgacggg
    13441 tgtcgtgagc acaccgaccg cgaccgcacg ggagcgaccg cgagggccca ccgacaggac
    13501 gcagtagccg cagcgggaca ggcccggatc gatcccgagg acgaacacct gtacgaggct
    13561 agtcgacctg ggccccgggg gaatgtgacc catcacagcg aaacgggtca ggtccgaccc
    13621 gcaaacggcc gatagctctc caaatggctg accagacatc tctcccaccc accgtgtcgc
    13681 ttcgcggcgt gggtcaccga ttcggcgacg tccaggcgct cgacgagctc accttcgacg
    13741 tgccggcccg acgcatcacg gtgctcctcg gccccaacgg cgccggaaag acgaccgcca
    13801 tccgatcgat caccggggcc ctgtcgccga aggccggcga catctccgtg ttcggcaccg
    13861 atccccgcgg cgagggcgac caggtgcgcc cccgttgtgg cgtcgtctcc gcgaagccgg
    13921 cgctctacga ccggctctcg ggctacgaca acctcctcta ctccgcacag ctctacgacg
    13981 tgccggccgc cgggctgcgc cagaagatcg tcgccgccgc cgagcggttc ggcatcgccg
    14041 acgccctcga ccaccaggtc ggcggctact cgaccggcat gaagacccgc ctggccctgt
    14101 cccgatcgac gctccacgat cccgatctcc tgctcttcga cgagcctacg tcgggcctcg
    14161 atcccgagag ctcgcaggcc gtcctcgacc tgatccgcga gatgacgaac gacggtaaga
    14221 ccgtcgtgat gtgcacccac ctgttggccg aggcggaagg cctcgccgac cacatcgtgg
    14281 tgatggaagc gggccatgcg ctgtgcaccg gcacccccga cgagctcacg gagcgctacc
    14341 tgccggcggc aatggtcgtg ctcgacgccg aggcacccga ccagctcgac cgcctcgccc
    14401 acatgaccgg ggtcatcggc tacgaccgca ccgacgtggc ccgcatcgaa ctcgacggct
    14461 tcggccgagt gcccgacctc gtgctgagcc tcgcccgcga cggggtacgc ctcacccgcg
    14521 tcgagcccca cgtccccacc ctcgaggacc tctacttcgc cgtacgtcgg gagcgaaccg
    14581 aactccttgc cgggcgtcca ctcccgaccc tcgacacccc cgatcctcag ggtttcggtc
    14641 gcgccaacgc ccgctcagcg ggcgtttcga cgaccgaaac ggtgggggcc ggccacgagg
    14701 aggtcagcgc atgaactgga aacgcatgtg gatcgtcgcc cgcaccgacc tcaagcagct
    14761 cgcgcaggcc aaggactact gggtcccgat ggtggccttg ggtgcactgt tcttcctggt
    14821 cgtgcccgcc atcctgctgc tcacgatcac caagatcggc aacgtcgaga tcgtcaacca
    14881 gatctccaac acgctgaagg tgctgcccaa ggcggcgcaa tcccagatca ggggcacctc
    14941 gcccgagggc aagacgagct tcgcgctcgc agtgttcctg ttcgcccccg ttgccgtggt
    15001 cgttcccctg acgatctcga cggcggtcgg cgcagcgacc atcgtcggcg agcgcgagcg
    15061 gggcaccggc gagttcctcg cccactcccc cgcctcggcc cgcgagatct acctcggcaa
    15121 gctcatcgcc agtctcgtcc ccggctacct gacgacgatc gtcgggttcg gcctgtactc
    15181 ggtgctcgtg aacgtgatcg ccggtcccga ggtcggcggc tggttcttcc cgacggcgca
    15241 gtggtggatc ctgatgttct gggtcatccc gccgttcctg gcattcacgc tgtcgctcgt
    15301 gctgcgcctc tcggcacggg tgaagtcgac cgcggctgcg cagcaggcct ccggcctcgt
    15361 gtcgctgccg ctgatcatga tcgcctacgg acagagctca ggcgccttgt tcggtgcgca
    15421 ggtctcgggg ttctacgtgg gcctcgtcgc ctgggtcatc gccatcgtca gcctcagtcg
    15481 cggcgtccgc tctgtcaccc gggcccgcct cctcggcgtc gccaacgagg tgtagccgca
    15541 cgggctgcgc tgcgacggag cgacggcggt gctacctgct cacgcttcgg cgacgagttc
    15601 ctggagcgga cggaagccgc tgcccggccc gatcgagcgg tgtgccccga ccaccacacc
    15661 gtcggcgtcg atgacgaacg tcgagcgacg atagaggtcg agcagaccga ggatgccgaa
    15721 cgcccggccg accgacttgt cgacgtcgct caacatcggg aacgcgaacc cgccgtgcgc
    15781 cttggcgaac agctggtggc tctcgggatc ctgcgggctg atcgccacga gctggatgcc
    15841 gtcacggtcg aggagcccga tgttggccgt gtagctcagc aactgctcgg tgcacaccgg
    15901 cgtgttgtcg gccggataga acgccacgat caacggcgcg ccggcgaact cgccgctcgt
    15961 gaagtgacgc cgctcccccg tcgaaccatc gatgccgtcg agctcgaaca ccggcgcacg
    16021 gtcgccgacg cggagcaccg gtgggttgcc cggatcgtcg gggcccccgt ccagcgccgg
    16081 cgccgcttcc ccgcccggct gactagtcaa gctcggccat cagggcgtcg gggatgtcga
    16141 agttggcgta gacctcctgg acatcgtcgt ggtcgtcgag cgcctcgatg accttgagga
    16201 tggccttggc ggtctcgagg ctgtcgatgg tgatgagcga cgacgggatc atcgtcagct
    16261 ccgccgacat gaccgcgtat ccggcggcct cgagcgcaga cgacacctcg tgggtggccg
    16321 aaccgtcggc cacaacatgc cagttgttct cggcgtcgcg ggagatgtcc tcggcgcccg
    16381 cctcgagcgc cgcctccatc agttcgtcct cgtcgacagc gccgtcgacc gtgacgatgc
    16441 ccttgcgctc gaactgccac gccaccgcgc ccggctcggc gatcgagccg ccgttcttgg
    16501 agaacaccga gcgcatctcg gggccggtgc ggttgcggtt gtcggtcagc acctcgacgt
    16561 acacggcgac accaccgggc gcgtagccct cgtagttgac ctcctggtac tcgacaccct
    16621 cgagctcacc ggtgccacgc ttgacggcac gttcgatcgt gtcgagcggc accgaggcgg
    16681 cccgcgcctt ctggaacatc gtgcggagcg tcgggttgga ttcccgatca cccccgccgg
    16741 cacgagcggc aacctcgacc tgcttgatca gcttcgcgaa ctgcttgccg cgcgccttgt
    16801 cggtcgcgcc cttcttgtgc ttgatcgttg cccatttgga gtgacccgac atggaagagt
    16861 ctccttctac gaactggtga tcgcgtcgag gaacagacgg tgcaggcgat catcggcacc
    16921 gagctctgga tggaacgtag tggccatgac ggcccccgcg cgtacgaaca ccgcattgtc
    16981 gccgacagcg gccaacacct gcacaccgtc acccgtgcgc tcgatgatcg gcgcccggat
    17041 gaacgtcgca tggaacggcg catccaggcc gtcgacgtcg atctcggttt cgaacgaatc
    17101 gatctggcgg ccgtacccgt tgcgccgcac gtcgatgtcg agtgtctcga agtcgcgttg
    17161 gtcggcgcgc ccgtccgcga tgtggctcgc cagcatgatg gctccggcgc acgtgccgaa
    17221 cgctggcatg ccggcggcga gccgctcgga gatcggctgg aacagacccg tggagacggc
    17281 cagcttcgac atcgtggtcg actcgccacc ggggatcacg agcgcgtcga caccgtcgag
    17341 gtgcttcggt tgccggaccg cgatcgtgct ggccccgagg ccttccagca tgcgttgatg
    17401 agcagcgaaa gcgccctgga gtgcgagaac gccgacgtgc ggggtcatgc cggaggtacc
    17461 ggagcgatag cagggagcga agcgaccgta cctagcgaag gtggcctccg gcagccagaa
    17521 atggctacca gccccgatcg gcgaacgtcg tctcgaggcc gccgatctcg aggccggcca
    17581 tcgacttgcc gagcccgcgg ctgaccttca ccaagatgtc ggcgtcgtcg aagttggtgg
    17641 tcgcttcgac cacggccttc gccatgatgt cggggtgctc gctcttgaag atgcctgagc
    17701 cgacgaacac ggcctgggcg ccgagctgca tcacgagcgc ggcgtccgcc ggggtcgcga
    17761 tgccaccggc gcagaacatg gggaccggaa ggacaccggt gtcgtggatc tcctgcacga
    17821 ggccgagcgg ggcctgcaac tgcttggccc attcgaacag ctcggcggga tcggcctgcg
    17881 tgatcttgcg gatgtcaccg aggatctggc ggaggtgacg aaccgcctcg acgatgttgc
    17941 ccgtgccggc ttcgcccttg gagcggatca tgcacgcacc ctcggagatg cgacggagcg
    18001 cctcacccag gttggtggcg ccgcacacga acggcacgtc gaacgcccac ttgtcgatgt
    18061 gatgggtctc gtccgcgggg gtcagcacct cggactcgtc gacgtagtcg accccgagcg
    18121 cctgcaggat ctgcgcctcg gcgaagtgac cgatgcgggc cttggccatg accggaatgg
    18181 tcactgccgc cttgatctcc tcgatcatct gcggatcgct catgcgggcg acgccgccgt
    18241 cgcggcggat gtcggagggc acccgctcga gggccatgac ggcgcacgca ccggcgtcct
    18301 cggcgacccg cgcctgtgcg gcgtcgacga cgtccatgat gacgccgccc ttcagcatct
    18361 cggcgaggcc gcgcttgacc tggacggtgc cgcgaagcga cgaaggagcg accggggagg
    18421 ctgaatcagt catgggccca gtgtatctct ccgcctgagg ccctccgaag cgagtaacgg
    18481 cggtacgccg tcaccagtac agcggccgaa caccgcggcc ggccgatcag ccggccagca
    18541 tcgaggccgc gacctcgggc gacatcggcg ccgccgtggc accgtcggtg aactcgaccc
    18601 aggttcgccc cggggtcagc cggatcggct ggccgtcagc gcccgtgagg gtgatcggct
    18661 cgagctggga cggacggttc cacgtgccgg tgaccgcggt gccgcccgca ccgaggaccg
    18721 tcaccttgcc ggtgccctcg gtcttcgccg tcggcgtctt ggaaccgccc cggtactgga
    18781 tctccatgat cacgacgttc gcagcgttcg cctgaccggt gccctcggtc tggtcgcgct
    18841 tgttgtactg ccaccggagc cactgattgc cggcggggtc ccacacccac gccgacggcg
    18901 tgtcgccgac cttctcggtc atgcccggca tcgggatcgc tccggcgttg gtctcaccct
    18961 ctccgaggta ctggaacaac gtcagcggcg gaccctggtt gggctcggcg taggcgaaca
    19021 gcttgtcggt cgacgtgaac aggttgtgcg gcgccttgcg accgctcttg cggaagtagt
    19081 ccttggggtg cttgtcccag ttgacgttga cgacccactc gctgcggtac acgtcccgca
    19141 cgacaccctc gttggcgccc gaccagccga agagcggctt gccgaggagg gcgaggatgg
    19201 gcgggtcgct gtagcgcgcc gagcgcgtcg ggccgatctc gccggcgtcc tggctgtgga
    19261 acaccgccat cagccggctg atgccctcca ccttgatctc gaagacgatg tcggcctggt
    19321 tgaggccctc ctggggcatc gcgtcgaccg agttgtcgat cttcaccgcc agcgccggac
    19381 gaaggagctt gaccgcggtg gcgtcgtcga tcggcaggcc cgtcaacggc gccgtcgccg
    19441 ccgcgaccgg cacggcggtc gtggtcgtgg tcgcctcggt ggtcgtcgtc gaggtcgacg
    19501 tggtcgtcgt ggccggagga tcggccttct tgtcgtcgcc gccacagctc gtcgccatga
    19561 gcagagccac ggcgcagcag gcggtcgatc ggagcgtgag gttgcgagga gatcgagaca
    19621 tgaggcccgc gaggctagtc cgatgacggg agggctacag ctccgccatg agcgacgcgc
    19681 gctggtcgat cggcgctcct cccccgaaca gggcagagag ttggtacgcc gtcgtgtcgg
    19741 tgtcggccgc ctcgaccagc gggtcggcga gccacaagtg ggcggtgccc cacgtcgccg
    19801 acaccacgcc ggtgccgagc gcctccatct tctcgagcgc ggaaccgagg cccggagggt
    19861 accgggtcat gtcgatcgcg cccaggtccg acaggaactc gcgctgatcg ccgagcactc
    19921 gtcgcagccg gccggcgcgc cacccggcga ggaaggcgac gtaccccatc gggcgaccgg
    19981 gacgccgcgg gccgttgcgc accagtgggc cacacaccag catcgctgcc tgcgcaccca
    20041 gctcggcatc gtggctccgg aggcgcacca gcgcctcggc gagcacaccc tcgagttcga
    20101 cgcggtcgag ctgctcgagc agacccgtgg tggcaatgac cacaccctcg tcgggcggcc
    20161 cgtagaccgc caggttcggg cgggcctcgt cgatcacctg cagcgtcggc atgaccgacc
    20221 ccgagctgat cgacagccct tccatgaggt tgtggtagcg ggggaactcc ggctcgggag
    20281 cggggcgggc cccgaacacg gtcgcgagcc ctgccgacgc cgacttgggg aggatgacgg
    20341 cgacgatcac acccgacagc accccggcca cgaggccgat ccaccaggtc gccaccagac
    20401 cgacgatgac gcccaccacg atcgccgtcg cgagcgcctg cggcacgacg aggaacttgg
    20461 tggcttcgcg attgcggtcg gtgcggtcga ggagcgtgcg cgcttcgata ctcatgacag
    20521 gaccgttctc ctcgcggaac tcatgcgccg aaatcgaccg cgacgttgcc ccgcgactgg
    20581 tcatcggcct ggaagtactg acgttcctcg aacttgaaca tgttggccac gaccacgctc
    20641 gggaacgact ggatcttggt gttgtaggcc cggacgttgt cgttgtagta ctgccgggcg
    20701 taggcgatac ggtcctcggt cgagctgagt tcggtctgca actgctggaa gttcgtgttg
    20761 gccttgaggt cggggtaggc ctccgacagg gcgaagaccg acttgagcgc accggtgatg
    20821 acgttctccg ctgccgcctt gtcctcgatc gagttggccg acatggccat gttgcgggcc
    20881 tggatcacgg cctcgagcgt gccgctctcg tgcgccgcat agcccttgac cgtggcgacc
    20941 aggttcggaa tgaggtcgtg ccgacggttg agctgcacgt cgatctgggc gtacgccgcc
    21001 tcgatcttgt tgcggagccc gacgaggccg ttgtacgtga acatgaacgc caacccgacg
    21061 atcacgacca gcgctatgag gatcaccagg acccagatca tgtgctctcc tcggaccgcg
    21121 gccgacagag cggacgcgat cgcccgtcca tgataggcac gctcccctcg ggaccgggtt
    21181 ctccctccac accagggcga tgagccctcc ggcgatcgac cgtcgtgtgc cgccagatct
    21241 cccgagcgca gatcacgcga acgcgccgtg tgagatcgac gcgccctgga cgcgtgatcg
    21301 ccaccgaccg tgagcgttcc agcgctcatt tcactgtcag gggtgcgatt taggctcggt
    21361 cgagcgcagc tcgactgcga ctcgaacctc cggttccccc aacacgagaa caaccccgag
    21421 aggcgaacgc gatgctccgg aacgacggac cccgcgtgat cgaagccgag cgtcctgcga
    21481 cgcagaccaa cgtgaggaca gaactggcac gcatcggccg gcagtggttg gtgtccatca
    21541 cccgcctcgt cgagctgtca gccgaactcg accagtccgg cgaatgggcg ctcgacggct
    21601 cccccacctg cgcgcactgg atcgcgagcg tgctcgacat cgaggtgagc accgcccgcg
    21661 agtggctccg cgtcggacgg gaactccgcc ggctccccaa gacgcggtcg gccctccggg
    21721 atggacggct gtcctacacc aaggttcgct ccgtcacgcg gctcgcgacg gccgagcacg
    21781 agggcgaact cctggacatc gccgagcgaa cggcaccggg ccaactcggc acggccttgg
    21841 cagcctggtc ggctcgcaac gaggacgagg ccacacggaa ccaacgccat cagcgcgacc
    21901 ggtccctcac gtggcgaacc gagcccgacg gcatggtgac cgcatcgatc cggatgaccc
    21961 cggaacaggc cgcgctcgtc atcgcagcga tcgacgccga ggtcatgcgg ggagacggac
    22021 gcgagaccga cccgaacacc gacaagaacg ccaccgacgg tgagatcgac gcgtccggga
    22081 cgcgtccatc tcacgttcgg tggtcgagcc tgggccagca acgggtcgac tcgttggtac
    22141 gtctcatcgg aggcgggggg tcagaggtca cgaccgaggt catcctccac gtacgagccg
    22201 acggatgcac gctccacgac ggcacgccga tcggaggctc gatcgtggag agaatcgcct
    22261 cggaggcctc gctgcgggtc atgatccacg acgccgagtc acgtccgatc aacgtctcgg
    22321 gaacgcaccg gcaccacacg aaacggcaga agctcgtggt gaaggaacgc gacgacgtgt
    22381 gcgtcgactg cggagggacg gacttcctcg agtacgacca cgtacccgac ttcgccgtca
    22441 cgaagcacac actcgtcgag gagaccgacc tccggtgctc ccggtgccac agagcgcgtc
    22501 attccaccgc cggcggtggc agatgaggtc gcagttccct caggcgttga gcgcggcctc
    22561 ggcgatgacg gcctcgtaga ggacggtgta cgcctcggcc aagcgatcca tcgagaaccg
    22621 attcgcgcgg tcgcgaccgc tcgcacgcag gcggtcggcc agcgcgtcgt cgccgagcac
    22681 ctcagagatg gccatggcga acgctgccgg gtccccccgt tcgggcagga gcgctgcggc
    22741 cggctcgccg tcgagcggac cggcgacgcc cacgtagccg ctgatcgacg aggccaccac
    22801 cggtgcgccg gccgccattg cctcgaggag gatcaccccg aacgactcgc caccgagcga
    22861 cggtgccacg aacacgctgg ctgcggccat ccgtcggttc cgctcgtcgt tgctgatgcg
    22921 gccgagccac tcgatgcgat ggtcggcagc atggcgcgct tgcaactcct cggtctcggg
    22981 gcccgatccg gcgacccaca cccgcaggtc ctggtcgatg aggcccacgg cgtcgagcag
    23041 gacccccagg cccttgcgct cctcgtggcg cccgacgaac aagaccgtcg ggcggtcgtc
    23101 gaccgcgccg tcggggccgg gcgggtcgac ccacggctcg gccgacgcga actggtcgac
    23161 ctcgactccg ttgaacagga tcgtccacgg ctcgggaatc gcgacgcctg ccagggcctg
    23221 cgcgtcctgg gagacggcga ccttggcgtc gatcctcgac ccgaaccagc gggcgaggct
    23281 cgacatcgac ttgtacatcg gctgctcgcc ggcggcgtgg aacgtcccga cgatcggggc
    23341 cgagcggagc atgagtgccg tcacggtcgg gccgggcacg agcggttcgt ggagatggag
    23401 gacgtcgaac ttctcgtccc acagcgcccg catggtgcgc aattgtgcct gagggttcgg
    23461 cgagatcggg gcgatggaac cgttggcggc gttcatcacc gatggcccga tgaccgtgac
    23521 gccgccctcg gtgggcggag cgtccgccgg cgccaggatc tggacgttgt gcccacgctc
    23581 gcgcagcgcc cgggccagac ccagcacctg gccctgcaca ccaccgggca cggtgaggct
    23641 gtacggacag accatcccga tgtgcacgac gtcgaccctg ctcagccgcg acggggcggc
    23701 ggcgtggtgc cgagcgcctc gtagtcgctc ggccagttcg gttgcaggag gtgccattgc
    23761 tcgggtgccc gggcgatgag gccttcgagc tcggtggcga gcacctgggt gaagcggacg
    23821 acgtcctcac ggatcgaggt cgctccgggc gtccgctcga tctcgatcgg agggcgcacg
    23881 acaccgtgca ctccacgttc ggtaaagtac acggcggtgg gcagcacccg gcagccgccg
    23941 cgcaacgcca tcacggcggg gcccgccggc agcgaggtcg tctcaccgaa gaaggtgatc
    24001 tcgggtccgg cgcgggtgag gtcgcggtcg cacagcaggc acacgatctc cttgttggcc
    24061 gctgccatcg ccacctcgtc ggcggcggtg ggactgagcg ggatgatgtt catgccgagc
    24121 gacgtgcgga actcgaggaa ccactcgaac agttcgggtg gctcgagctg ctcgaccacc
    24181 gcggccaggc cccagttccg aacctgggtg atccagaacg ctgcccattc ccagccaccg
    24241 aggtgtggca gcgcgagcac ggggccgacg gcgtcgtgct ccatcgcttc ctcgatgtgc
    24301 tcgatgccct cgacggtgaa cccggcggcc acctcgtcga ccgacattcc ggtcagacgg
    24361 aacgagtcgt agtagtagcg ggcgtagctc tcgaaggtcg cctgcacctt ggtgttgatc
    24421 tcgttgcggg tcaggtcgcg cccgtagaca cggcgtaggt tccgctcgac gatgaggcgc
    24481 ttgtcgccgc cgcgcagcgc tgcggcgtgg ccggcggcgg cgagcacggg ggcgccgatc
    24541 tggcgcgggg cgcgttgggc aagcgcttcg ccgagtttgt aggcgcccac gatcgctgga
    24601 ccggcggcgc gctccagcgc cggcgcgagg gccttgcggg ctgcggcgcg agcggcgccg
    24661 atggggtcga gcggggccac cgatcagtag ccgcggcgag tccgggctcg ttcgcgggcc
    24721 tcggcgcgac gcgcctgcca gcgcgcagcg gccggcgacg gcgtcgaggc acgggagcga
    24781 cgacgggcgg cagcgggacg gggcatgggc agcggcttcg aggcctggtt ccacaccttc
    24841 acaaaacgct ggatcgcggt ggccatgttg agcaccacga gcaccacgag ggcggcaatg
    24901 aggaaccggt cgaagaggag gccggcaccg aggatcacga atcgttcggc ccgctcgacg
    24961 aggccaccgc gggcgtcgag gccgagcgcg tcggccttgg cgcggatgta ggacggcagc
    25021 acggccgtac cgaacgcagc gaaggcgagc accgggaggt atcggctctg gtcggtctgc
    25081 gaggcgaggt accaggcgac gccaccgaac aggagcgcat cggtgacgcg gtcgctgacg
    25141 gaatcgaaga aggcgccgcg ctggctcgac gtgcccgagg ccttggccac ggcaccgtcg
    25201 agggtgtcgg gaatgcccgt gagtgcgagg aggatgacac cgaggcggag cgcgccggcc
    25261 ccgacggcga tggcgcaggc gaccgacatg gccaggccga cggcggtgat ggcgtcggcg
    25321 gggatgcccg cgcgcttcac gctcatgccg actggcttga cccaggtctc gatctggccg
    25381 cggaagcgtc cgtcgagcat cgtgactcct tcagctgtcg tcagcgggtg ctaactgcag
    25441 caagcatagg ggaacgttca ttcagcgtac agccactgcc gcgggcgagt ccggcactgt
    25501 tgccgcatcg acccacctcg accgttgagc tgtcggtgaa tccggccacc ggagcagtca
    25561 ggaagcggag gtcgcccagt cctcggggtt ctgctcggcg aaccactcga cgacctcgcc
    25621 ggagatgccg tcgacgacga cgatcgcccg ccgcgacggc gggttgtccg cggagtaggc
    25681 cagcaggcgc cacaccggac gcgacagcca acctcgccac accatctgcg cggacgcgtg
    25741 gccgacggtg aaccccacgg tgcggttggc ggtcgcgagc gcctccgtct cgtcgacgag
    25801 cagggtgcga cctgcgatga ggccgtagat cgcaaagcca ccgaggaaca cggcgaccca
    25861 caggtacccg tcgttcacga gcggcgaact gtcgcgcttg gtgatccaca acaggaggca
    25921 caccgccgag atcaacaggt agatcgccgc ggggatacgg cgacggttgt tgttgggcag
    25981 gaggtagtcg gccgacgcct cggcgacgtt gaggtcggcg ggcagttcgt cgacgacgtc
    26041 ggcgtcatcg atctcgggcg tggactcggg ggactcggtc acagcgccga gcctaggagg
    26101 agacgcagcg gcgccccaat ccgctcgggg cgaggtgtgg accggagcgc acgaattccg
    26161 tgcgctccgg ttcaaacctt cccgccggat cagtggtcag ggccgctacc agacggcccg
    26221 gagcgaagcg agcgcgtcgt cgagcgacac ggggagcacc ctggtctcgg cggcgacgcc
    26281 gatgaagttg gcgtcgccgg tccagcgcgg cacgcagtgc acgtggaggt gatcggactg
    26341 gctgccgccg gcggcggccc cgaggttgac cccgacgttg acggcgtcgc accggaacgc
    26401 cgccttgagc gccaccaccg acgagcgcac gagcgcccac agctcgatgt gctcgtcgtc
    26461 ggtgagcgct tcgaggtcgg cgacaccacg gttggggagc accaacaggt gcccggaggt
    26521 gtaggggaac ttgttgagga gcacgaagca ggtgcggccg cggtgcagca ggtgcttctc
    26581 gtcgtcggtc ccgtccgcgg cgaggatgcg ctcgaagagc gtgcgtccgt cggtgggcgc
    26641 gtcggagcgt gactccaccg acgacgtgac gtaattggag cgccaggtgg cccagaggtg
    26701 gtcgagcggc atcgtcggga ccgaaccctc agacgccgcg gaagtccgcg acctcggccg
    26761 tgacccgttc ggtgaacgcc gcgaccgcga cgtcgcgctc gacgtcgttg cctcgggcgt
    26821 tcacgccgac ggtgccattg gcgacatcgt cgtcgccgac gacgaggacg tagggcagct
    26881 tctcgccctt ggccttgcgg atgcggttgc cgagcgattc gtcggcccac acgatgtcgg
    26941 cgcgcaggcc gacggccttc agcgacgaca gcacctcgga cgcgtagtcg gcatgggcat
    27001 cggcgacggg gagcacccgc acctgcaccg gggcgagcca ggtggggagc gcacccgcgt
    27061 agtgctcgag caggaccccg aagaagcgct cgaccgagcc gaacagcgcc cggtggatca
    27121 tgaccggctg ctgacgggtg ccgtcggggg cgatgtactc gatgccgaag cgctcgggca
    27181 ggttgaagtc gagctgcagc gtggacagct gccagctgcg gccgatggcg tcgcgcacgt
    27241 cgacgtcgat cttggggccg tagaacgcgc cgccgccgtc gtcgacgatg tagtcgagcc
    27301 cgtgggcatc gagcgctgcc cggagcccgt tggtggccag ctcccacaac ttgtcgtcgc
    27361 cgaccgactt ctcgagcggc cgcgtcgaca gcttggcctg gaagtcggtg aagccgaacg
    27421 agcgcaacac gtcgagaacg aaatcgagca gcgacgcgag ctcctcctcg acctgctcga
    27481 gcgtgcagaa gatgtggctg tcgtcctggg tgaagccgcg gctccggagc agaccgtgca
    27541 cggcccccga cagctcgtag cggtacacgg tgcccagctc gaacagccgc agcggcaggt
    27601 cgcggtagga gcgctgctgc gaccggtaca ccatcacgtg gaacgggcag ttcatcggct
    27661 tggggtagta ggtcgcgccg tccatctcca tgggcgggta catggagtcg gcgtagaagt
    27721 cgaggtggcc cgaggtctcc cacagcaccg acttggcgat gtgggggctg aacacgaagt
    27781 cgtagccgcc gtgctcgtgg cggaagcggg agtagtcctc catcgtcttg cggacgagcg
    27841 cacccttggg atgccacacg gcgaggccgg gcccgagctc ctcgggccac gacaccaggt
    27901 cgaggtcgtg ggcgaggcgg cggtggtcgc gcttgatcgc ctcctccaga cggtgcaggt
    27961 gcgcggcgag gtccttcttg ttggcccacg ccgttccata gatgcgctgc agcatcgggt
    28021 tcttctcgga gccccggaag taggcgccgg cgacccgcat gagcttgaag tggccgaggc
    28081 gaccggtgga cggcacgtgc ggaccgcggc acatgtccac gaacgccggc gtgttgcgat
    28141 agaagctgat ccgctccccc gccgacacct cgccgtcggc atcgtcgccc gacgccgcca
    28201 ggtccatgaa ctgctgcttg tagggatgcg cggccatcag ctccttggct tcgggaaccg
    28261 gcagctcgaa gcgctcgaag gtctggtcgg cggcgatgat ctcgcgcatg cgggcgtcga
    28321 tggcggcgag gtcgtcctcg gagaacgtgg ccccctcggg aagctcgaag tcgtagtaga
    28381 agccgtcgtc gatggcgggg ccaccggcga acgtggcacc cggccacatc tcgaggacgg
    28441 cctgcgcgag cacgtgggcg gtcgagtgcc ggagatgttc gagggcctcg ggatcgcggt
    28501 cggtcacgaa cttcacggtg gcgtggtcgg gaaggggtgc ggccaggtcg tggggctcgc
    28561 cgtcgacatc gacgacgagc gctgccttgg ccaggcccgg accgatcgac tcggccaggg
    28621 tggtgccggt cgcgccctcg gggaggctgc gttcggaacc gtctgggagc gtgatcgtga
    28681 tgtcggacat ggcgagcatg ctaccggcgc cccttcgagc ccccgtgcag tttgcccaac
    28741 ccccgagacg agttggtgac acactgttat gtaccctgct ctcacgcagc taccagcccg
    28801 gagtgtgatg accatgtctc gtaggttccg ctcgatccca ctcacgctg
//
LOCUS       DAOSOA010000009        28699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_1648_length_28699_cov_7.619362, whole genome shotgun sequence.
ACCESSION   DAOSOA010000009 DAOSOA010000000
VERSION     DAOSOA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 28699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28699
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1648_length_28699_cov_7.619362"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            585..6161
                     /locus_tag="PKY13_01515"
     CDS             585..6161
                     /locus_tag="PKY13_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOT78628.1"
                     /translation="MTTSLYWGLYRAEYAFHVLALGGQFASFADVGWWPPASAEGPAG
                     GWYPFPGLTYGHFTNTTGAPVRYQLKNWGARTFAGAGVRWESIVTVTGGDGSADPLCA
                     ALRPDGAARGDSAPGNLRVSDPVDTATGNLYHGFTDLDATASLPGLAVQRSYNSTDTD
                     GAALGSGWTEAYGATATDFVSYADVKLDDGRVVRFPANAAGTGFNRPQELAAELSRDP
                     DSSLRLTFNGGVVWEFDTAGRLEKTTDAAGVSATVVRDGSGLVSTVTSSAGTAPNQPK
                     LSFSYSGGFLVSIAATDGRSVSYGYTSGRLTSFTDAAGKVWAYGYDAAGALSTVTDPT
                     GVVLMSTVYGSSGRVVLQTTPDGGEETFSYDDVNRITTHTVVATGEVLKYVHDTNGRV
                     VQIIDPNNKTTGKQVGSFGFWNQSTTRTGVTRGSTLDARANVSATTDPASGPTSATYD
                     SQDRPLTITTAASGTTTYSYAGSGTSEIPSSETDALGKVTTRTITNGLVMSETDPDGV
                     VRSYTYSPTRQVLTSTVGGATTTMEYWPQGWLKKVTSPEGRATSYTYDGEGRVLTVTA
                     PGNAAGTTEVTTYTYDGAGRLLTTTDPTGAVTTRTYDSLGRLATESLPGKLATTYGYD
                     ALGQLTTVTDPTGVVTETHYGVLGRVEWTKDGAGRQTNYSYFDDGQLWKTTAPDGGVT
                     ENTYDSAGRLWKVKDPMARVTTTTYNAAGQVWKVTAPDGGVTETEYDAVGRVKKVTDP
                     TGRISQTTYTDAGRTQTTTDAAGVVTTLTYDSSGRAWKSADPLGNTTITEYWPDGLVK
                     KQTSPSGLATNVAYNNAGRQSVVTDPAGVTSTTTWSARGERLAEQISGRGPKQFLFNP
                     DGTMAWSKDERGNQTTYAYDGAGRSTGRVTPVATEGWGYVNGEMVSYIAPPVGGVARV
                     TLFGRDAAGRVNAVADPSGRAMNDTFNAAGDLTGRVFTQGGSTLGFTYGYDSAGRQNS
                     AVTPDGTFTKTFDLAGRLLSVNPVDNRYMSYSYDSGGRVSQVTTPEGLELVYGYDAAG
                     RVQKISPNSTMVDWFNGANAAGADPSKWTRQAVGGGSAAIDGNRLRLSTTATAGSSMG
                     VTSTAPQTADSVTTVSFQAASSAVGNQSRFTVGVRQNAANTAGYRVEFTTDSTTAKLI
                     KRVAGTDTVLGTFTAPTPGTEIRTQIEVSGSTVRAKVWPASGSAPGTWGVSVTDTSIT
                     AAGATQVRTDRVAGSNSVAIDSYRQRNNQGSALAPFVTNTYNADSQVTTEAFPSGSRV
                     NTFTAGRLTKQVQTVPGADRTTDVTYDSAGAYATTAVGGVTTTYGYDASGQLLSATPS
                     TGAALEYTYDGAGRRATSKVGSVTTSYTYNSASQLTAVTPAAGSATSMTYDLAGRRLT
                     ETTGSAVTTNTYDQAGRLAGIAKTNAGTVVTSEARKYTPEGLLRMDTVTGPGGGFLRA
                     LKYDWDTNTGGLAQMVSTLDGETNFGLVRNNGTWAASTKGGTPTLFGSDVFGSVINST
                     GQTLANATGWDPYGNPTGATATNAARFGYRGELTVLGDTYLRARNYQNTTGTFTTVDP
                     LDDVAATSTSGNRYHYSYNDPINRQDPSGLRPGEPDHEHQAGRPTGSAGASAAGSSDR
                     VRRSVEDAIKRCPAPEDHIQRATTKWWYDIDFAARTCTLTFNGKMSNGLEQLIVDYQT
                     EITVGLAVVAVAAAAVAVAAGGGAVVLSEAGKASVEVVAGGSALVVGVAVAGGVVTVV
                     VGGVIVVLRNLDAAGPSPQTSGRGGGSTTTSTPSTCGATEDGPDESGKGRSASPVGEV
                     LRDGAVKVVIYSNDHGPPHAHVKGGGPETRVGQNGKPLNGDPELTRAQRQVVEKHLKE
                     IRDAIREYMRWFGDEC"
     gene            6973..7746
                     /locus_tag="PKY13_01520"
     CDS             6973..7746
                     /locus_tag="PKY13_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOT78629.1"
                     /translation="MSVRHRDGVASRGPDDLIVEEPMEIRLDDHLVTTTMRTPGHDFE
                     LAVGFCHSEGLLGGAAVNGIRYCGTGTAVDTEFNVVSVSTGGWAPEPTARVGTTSSSC
                     GMCGSMAIEQMTDRLDPMQPVEFDWTLLATVAERIGRQQELFAQTGASHAAGAFRLAD
                     GRVDVVREDIGRHNAVDKVVGRLLLDDALPALRADAEPMGLWVSGRASFEMVQKAWAG
                     GFRVLASVSAPSSLAVDLAKQGGVSLAGFARGGDLTVYA"
     gene            7757..8500
                     /locus_tag="PKY13_01525"
     CDS             7757..8500
                     /locus_tag="PKY13_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT78630.1"
                     /translation="MPDEESTEPTIERVLVIMAHPDDVDFGSAGTVATWTDAGLEVTY
                     CLVTSGDAGGSDLTQSYEDRASLREAEQTNAAAEVGVTDLRFLRLPDGRLEYDLDLRK
                     AITRVIRQVRPHRVLTQSSERNVDRIYASHPDHLAVGQAAIAAVYPDARNPFAFPELL
                     ANEELEPWTVPEVWTQGGPEIPEPARVVVDITDTFDRKVAALRAHVSQTGGREGLADF
                     LREWAVDNARIGGLPEGRLAEVYRRIDTR"
     gene            complement(8502..10490)
                     /locus_tag="PKY13_01530"
     CDS             complement(8502..10490)
                     /locus_tag="PKY13_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78631.1"
                     /translation="MSTRMRPFIILLTTVALLAGCSSDDGADDDRAGAASDRAATTAR
                     PAPVSEPPGTATCDDTDPTACLLPWPNDRFTRADPSTPTGRRVDLPVDGTPVNADGVH
                     IDPTEWNRNDGFSPASNLLVHVESLDVSASKLPPVTDLSRSLDDDSGLVLVDVTTGDR
                     VAAWAELDANAEDPDQQALIIVPATSLTEAHRYAVGLRGLVRTDGTEVEPTGAFSELV
                     DDPDEEQQEWLDALGTAGATTDDLDIAWSFTVASSDSLSGRLRAMAAETLSEFGDGAP
                     PFTVTSNETAGAARIVSGTFEMPRYLAGDGGPGSVLNNDNDPDGIPARNGTMSADFTC
                     TVPAGATAADPAPMIVYGHGLLGSRSEVLGIGGTAAIANIGMCATDYLGMSAADIPTI
                     VGEFRDFSGFRTQPDRMQQGQLAFVVLGRLLRSADGFATDGAFSTDGAPVIDTSKLAF
                     LGASQGGILGGVASTVTDDWDKVVLAVGGLGYNLLLRRSVDFDEFIGTFEAAYPGELD
                     QLLTLELAEQLWDRGENAGYAQHLTTDPYEGIAAKPVLALEAFGDHQVTNVSTQKLAR
                     TLGLRRREPTLAAGRSSAVDPFYGIAPLPELPTTGSALFVWDFGTPTPPDTNTPNRGG
                     DDPHGDLGDETSALALLVSFVNDGTLIDVCGEGPCTGG"
     gene            10570..11259
                     /locus_tag="PKY13_01535"
     CDS             10570..11259
                     /locus_tag="PKY13_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HOT78632.1"
                     /translation="MNASTPQVGLAVAHFFWSTSVLADADAVLDVIAKAEADGDQVVT
                     ASMLGHKSDLCTMVLSTDQWRLRRFQTAMVEAGFELVDSYLSLTEMSEYAQGLPEEMK
                     QARLFPQLPPEGKPAFCFYPMSKRRNVGQNWFTLPYDERKALMMEHGASGRKFAGRVV
                     QVITGSTGIDDFEWGVTLFCQHPDDVKEVVYTMRYDEASATYAEFGSFYVGMVASPVE
                     VLSSLGVTNAD"
     gene            complement(11275..12621)
                     /locus_tag="PKY13_01540"
     CDS             complement(11275..12621)
                     /locus_tag="PKY13_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT78633.1"
                     /translation="MGAATASCTNAKLDESSSAAVTTTTAATEFGEGTDAATIDPGGG
                     RTGPITSGSSPLKGMKGAAPGIRITEDFRDRVRAVDGTVDDFTGAAEGYDIVMVLAIS
                     AEAARSDSPGRLADEVISSTEGGRTCLGFHACREFALDNTDFDYDGVSGNINLLANGD
                     PGEAGFAVVEFTGTGALRAVDQVTVQSRPLADRPAGADPIYGPIGNGVLEIGTLLPVM
                     GPDGAAARAALAGAKVAVDEVNAEGGVLGEPVVLVPDESGDGSSSATTDAVRRLIDRP
                     VDAVIGGTVYGITSVAIPELTDAGIILFSPTDTARALSVADDRGLFFRLSPPTDLEGQ
                     VLGNLITNDGYTRVAIAAGTGDDNQELAADIAAAINADSGTVTATVEVGDDPDGAAVA
                     QQLIDSDPQALVLVTSAPAAASVVRGLVAKGKGPSTIATYGTAADMTDELAQLVGG"
     gene            12762..14321
                     /locus_tag="PKY13_01545"
     CDS             12762..14321
                     /locus_tag="PKY13_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT78634.1"
                     /translation="MTELFQPLARDLGDAPALIDEAGTTSWRQLDERANRIIGALRAR
                     GVGDGATIAVMVGNRREFFEIFAAVCHASWVAVPVNWHWVGAELAYVIGNSGASAMIV
                     DEAYLDVARDARGREESANCEHWIALGGDVPDDFESFESLLAAASPDEPDGQATGGPM
                     FYTSGTTGFPKGVRSTLSTTGLPASMWTLISGSMLQMLGVPADGVTLLDGPAYHSAQW
                     VFSMFPLFGGSTVVMRHRFDPVETLAAIDRHGVTNLHLVPTQFVRLQKLDDHQRATFS
                     GESLQVVYHGAAPCPPDVKTAMLDWWGDVVHEYYGGTEGGFLTTIGGAEWRARPASLG
                     RPTDLTELRVVDDNGADVPAGTSGQIYFRSRIGSDFSYHEDPDKTTAAHLDGWGTLGD
                     IGYFDDEGYLFMSDRRIDMIISGGVNIYPAEIEAALVAHPLVADAAVFGIPDDEMGEQ
                     VKAVVELVDGAIADAALTDELIAHCRGRLAGYKAPRSIDVTDRLPRQPTGKLYKRLLR
                     DPYWTDVGRSI"
     gene            14377..14952
                     /locus_tag="PKY13_01550"
     CDS             14377..14952
                     /locus_tag="PKY13_01550"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HOT78635.1"
                     /translation="MTEPAEAQPETLVGISFPDIFRAQEFLTAATRMASRGELALKDA
                     VTVVKNSDGKTVVHETVDLQTGQSAMSGALWAGLIGLIVGGPVGWVAGLAVGAGAGAA
                     TAKLVDLGLADEWVEWFREVVQPDTATVALLVTELNRDALVTEASRFTGAELVYTNLD
                     PSTMDRVKQALGDHTAAPADAEAPPTTAPSD"
     gene            complement(14974..15855)
                     /locus_tag="PKY13_01555"
     CDS             complement(14974..15855)
                     /locus_tag="PKY13_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOT78636.1"
                     /translation="MAAQNYYEILGVKRTASADEIQRAYRKLARKWHPDVNTEPGAED
                     RFKAISEANEVLSDPEMRKKYDAFGEDFRKVPDGTDPSMWNRAGRMAGDFGFGGGATP
                     NIEDLLGGLFGGGRRATGPRPGADQQVEIELSIEESYHGGERSITLSGPRGTRSVNVN
                     VPVGIVEGQRIRVRGAGGGPAGDLFLTVRFAGHGRYTVDGRDLSVKVPVAPWEAALGA
                     KVSVDTPGGAATIKVPAGTSSGRRLRLKGRGLPNPQGAAGDLFAVVTIVVPKELTERE
                     AELFTLLAEESSFDPRA"
     gene            complement(15896..16189)
                     /locus_tag="PKY13_01560"
     CDS             complement(15896..16189)
                     /locus_tag="PKY13_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78637.1"
                     /translation="MRIQAIAVFERVVHQCHALSLDRPERPVLEVDAILREGDAEGEL
                     LLPVPTFMALLGGPAKANDALQHLAVNGRIVDHQEIAHIRFPLWERIDSSATG"
     gene            16274..16732
                     /locus_tag="PKY13_01565"
     CDS             16274..16732
                     /locus_tag="PKY13_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78638.1"
                     /translation="MSDESDARLMAEISARLVEASARALGPWVETAVAQRAGDRAAEL
                     ASAATAAGERCAEEVPATMTALLALDIDAQRSTPLGILRSACRYPAAVLAAAGVPAPA
                     RDPFDERANPDDRYEVGPATWADLGEEVGELGIAWGAAKAHLHKRRHLGR"
     gene            16824..17162
                     /locus_tag="PKY13_01570"
     CDS             16824..17162
                     /locus_tag="PKY13_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT78639.1"
                     /translation="MKLVTAIIKPFKLDDVKSALKAINVVGLTATEVQGFGRQGGHTE
                     TYRGAEYKIDFVPKVRLEAVVADDDVNAVVDAIVGSARTDKIGDGKVWVTTVDQLVRI
                     RTGERDNDAI"
     gene            17162..19465
                     /locus_tag="PKY13_01575"
     CDS             17162..19465
                     /locus_tag="PKY13_01575"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOT78640.1"
                     /translation="MPPVLDRSSVLDNPDLRGRPFVDAYTRLVDTWIVELYDELFGER
                     SGVALVATGGHGRGELAPQSDLDLLLLHDGSIDLGTAQRLWYPMWDAKLKVGHSVRTV
                     AETLALASDDLPTATSLLSVRRLAGDASLATDLAVDSAAQWRKQSKRVLASLMETVTS
                     RHETTGEVAAALEPDLKEGRGGLRDVHALSWILAAAPDLDIPRPESLAGEYDTLLNAR
                     VELHRVTGRAGDVLQLHDQDGVADRLGIENADVLMTEVATAARRIAWSSDEAWFVVRG
                     SLSGGIFKRSPRRQDLGHGVELCGERVHLAEGAVTDELSVLTVAVAAAKHRARIGRTT
                     LVTLQSAPPLPEPWPAAARALFVELLRCGPAAISVIETLDLNDLWLPMVPEWEPNRSR
                     PQRNAYHRFTVDRHSWECVALAAGLTDRVTRPDLLVMGALLHDIGKGYPGQHSIVGME
                     LGAAVMRRMGYQADDIDTVVLMIEQHLLLPDVATRRDLDDATTILLVAARVETTERLR
                     LLAALAEADSIATGPSAWSPWKAGLLATLVERVAFVTDGGDVEQVITASPATLAQLEL
                     VERGAAGGRPAIDAHDDRLTLVCSDRPGLFSRIAGVLALNGLDVLEASAHTSGDLAIE
                     EFRVVSAFGGEIHWSKVERDLERALLGRIALEPRLAERARSYHRRRNTGRGLEPNVRV
                     LENEASDGSTVVEVVGPDGVGLLYALTRALADLDLDILRAKIATMGSDVVDSFYVRDR
                     SGAPITDADDGAEIRTALLHVLKANAS"
     gene            19462..20172
                     /locus_tag="PKY13_01580"
     CDS             19462..20172
                     /locus_tag="PKY13_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOT78641.1"
                     /translation="MTDVPAPDDLTPDDSITEADLLRWAHALSGIARTGLGFTESRYE
                     RERYEEVLAVAADILAASSRSHDRAALVDEWLKMVGVGIAGYVTPKVAIGAVVGNDAG
                     EILLIQRADSGIWLYPTGWADIGYSPAEVALKEVHEETGIVAEVRGLLGVFDGQRRAF
                     SRIPLYSLVFHCHAVGGELEPHPLECLDVGWFARDALPEPLASGSLWVQAAFDAIDGK
                     VVPSFFDAPRGEVWNHPA"
     gene            complement(20191..21033)
                     /locus_tag="PKY13_01585"
     CDS             complement(20191..21033)
                     /locus_tag="PKY13_01585"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOT78642.1"
                     /translation="MSSLRWSSQPSLNQPIIVAAFEGWNDAGEAATEAVRRLREAWGA
                     VAFVDIDPEEFYDFTAVRPMVQFDDDGSRRILWPENEFFFAEPAGSPAVIVLRGIEPQ
                     LRWRTFCEQILDVAAAVNARLIVTLGALLADVAHTRPTSVFGTAHDPKVAAALHLEAS
                     RYEGPTGVVGVLHELCFSRGVNSASLWAAVPSYVPAAPSPKAALALIERVCSILGTTV
                     PDSDLPLAVAEYERQISDLVDEDEETAAYVAHLEETYDRDEIAGDTADELVSEVEKFL
                     REQR"
     gene            21131..21592
                     /locus_tag="PKY13_01590"
     CDS             21131..21592
                     /locus_tag="PKY13_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT78643.1"
                     /translation="MALDPTVTVRELTDADDITDAVVDAFARLIPQLSSSSPAPSRDE
                     LIAMATADADHLLVAEDVDGAILGSMTLIVFRIPTGVRAWIEDVVVDESARGRGVGEV
                     LNRTALDLAYGRGAKTVDLTSRPSREAANRLYQKLGFELRNTNLYRHNPLA"
     gene            complement(21613..22881)
                     /locus_tag="PKY13_01595"
     CDS             complement(21613..22881)
                     /locus_tag="PKY13_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOT78644.1"
                     /translation="MPLRRALAPLAVPILVLAGCSNDEGSDDTTVPTAAVSPARSSTT
                     GPAATTTAPTPTTPADLSRLTVRLTEVAAVESPTAMVPRPGSDDLFVGERAGTVRRLV
                     RDGEVFTTAEGAVLDLVADVADLGGERGLLGLAFSPDGAVLFVSYTDGSDDGASVIAA
                     YAMAGDVADVGSRREVLRLPQPFANHNGGNITFGPDGYLYVGFGDGGSQGDPDDNGQD
                     LGTLLAKLLRIDVGASISDGGYTVPDDNPFVGQAGVRNEIWSLGLRNPWRFSFDRATG
                     DLWIADVGAADWEEVDVLPASEGAGRGANLGWSLREGAHDTGKTGDRSVAMVDPIFEY
                     SHDEGASITGGFVYRGTRVPELNGVYLFSDFAAATLRGLVVTDGRLDQQAVIPTTGDS
                     MSQVSSFAEDAAGDVYVLNLSGEILRIDGA"
     gene            complement(22919..23779)
                     /locus_tag="PKY13_01600"
     CDS             complement(22919..23779)
                     /locus_tag="PKY13_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HOT78645.1"
                     /translation="MDQAAVDSLITLLDLEPIEVNIFRGESPDEDRQRTFGGQVAGQA
                     LVAASRTVEGMTVHSLHAYFLYGGDPSSPILYEVDRIRDSRSFITRRVKAIQHGRPIF
                     GLTASFQRHEEGYEHQMPMPEGQPDPESLPDFRERMAPWKDKLGDWYDRPRSIDTRYS
                     DWRSPEDHTPGPMFENMWFRANGKLPDDPVLHQCVVAYASDISVLDTSLRPHGLSASL
                     GEVFMASLDHAMWFHRPFRADEWLLYAQDTPSASGGRGLGRGLIYTREGALAASVVQE
                     GVIRPARWAR"
     gene            23871..24383
                     /locus_tag="PKY13_01605"
     CDS             23871..24383
                     /locus_tag="PKY13_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT78646.1"
                     /translation="MKLTPSGLLEHAKSDEGRKQLRYAGISVVFVPLGQAFVQILKWV
                     FDVHEVLAVLISACILTLPNYLANKHIVWRHKNKDNQATEITVFWLAAVLGTAFAMGF
                     VFLAAKIVPEDKGEVAHAIAIFAAQLLGYGIVWVARYLFLDRLIFKATHHGEEPSPEL
                     LDDLHRDFPV"
     gene            24398..25063
                     /locus_tag="PKY13_01610"
     CDS             24398..25063
                     /locus_tag="PKY13_01610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT78647.1"
                     /translation="MDPDLRARAEAARGFMPPDEGLALFAAGTDACRRVPDAPLLEIG
                     SYCGKSGIYLGAAAADADVVLFAVDHHRGSEENQPGWEWHEPDLVDPAVGRMDTLPLF
                     RRTIFDAGLERHVVATVADAPTLGRFWTTPLALLFIDGGHGAEPAHRDYEQWTPFVAP
                     SGILAIHDVFPDPADGGRPPFEIYERALESGNFAELGATGSLRVLERIDNGRVHRPEA
                     KAF"
     gene            complement(25083..25373)
                     /locus_tag="PKY13_01615"
     CDS             complement(25083..25373)
                     /locus_tag="PKY13_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78648.1"
                     /translation="MTDVRRVAVVATSLMLVVAGVLMTATVAGAQAPTTTLVPIDEQP
                     INSPSIIPEPNSTSQPTQYVVMIAVLLGLAAIALLVYRESRKKAGNRTHKPG"
     gene            complement(25366..26439)
                     /locus_tag="PKY13_01620"
     CDS             complement(25366..26439)
                     /locus_tag="PKY13_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78649.1"
                     /translation="MWLHDVDGIVTAVQLGETVDYIASVQLPSGMVPWYDGGHADPWN
                     HTEALMALALGGRVAEAERGFDWLVSTQRTDGAWHQYYIGDGVEQDKLDANVVAYVAA
                     GVWHHWLLTRDRGFLETMWPVVDAAVGFVLDLQTPRGEILWARHADGTPWTFALLTGS
                     SSICHSLRCAIAIADELGHGRPDWELSAARLARVIRDEPDAFAPKHRWAMDWYYPVLA
                     GVIGGDAGRERLDERRDAFIMDGKGVRCVSDRPWVTAAETCECMMAMLSVGQTDAAHD
                     LFRWAQRHRTDDGRYWTGIVYPDEVHFPGGEQSTYTAAAVVLGADALAGASPASHLFV
                     DHDRFPPLIDTHTDDTVADRSGD"
     gene            complement(26426..27187)
                     /locus_tag="PKY13_01625"
     CDS             complement(26426..27187)
                     /locus_tag="PKY13_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT78650.1"
                     /translation="MLTVDYELFDLRPGMVVLDMGCGAGRHAFESFRKGARVIALDYS
                     FDELAGVRDLLWAMRDAGEVPEANEAQAVRGDALCLPFADDTFDRIICSEVMEHLQDD
                     AGAMAELYRVLKPGGRIAVTVPAWLPEKICWTLSAEYHAPLSQGGHVRIYTEDLLRHR
                     MAAVGIETGESHRAHALHSPYWWLRCAISPTNDTHPLVKKYHDLLVWDMVKAPFVTRF
                     AERVLNPVIGKSVVVYGTKPTAAAGAHRRSTSVAA"
     assembly_gap    27277..27376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27377..28362)
                     /locus_tag="PKY13_01630"
     CDS             complement(<27377..28362)
                     /locus_tag="PKY13_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT78651.1"
                     /translation="MQTGTDAGPNSRRRPGPDDPLRIAYLTYRGKPHVGGQGVYTRHL
                     SKALVDLGHHVEVLSGQPYPIVDERVPLVELPSLDIYNDHFPMRMPGLWELKTWMDFA
                     EVTAFSTGTFPEPLAFSLRAWDHLKRRTHEFDLVQDNQCLGYGLLAIEKSGLPVLGTI
                     HHPITVDRRIEMEHAETTYQKYAKARWYAFTKMQTRVAKRLRRIITVSQNSYDDICRT
                     HDVSPEKLFVVPVGVDPDLFKPLPGVERRPHQIISTASADVAMKGQRFLLEALAKLRT
                     EYPDLKLVLIGRLKEGSIAQRTIEELGLWDAVEFVSGVSDERIVELYNESACA"
BASE COUNT         4492 a         9508 c        10034 g         4565 t
ORIGIN      
        1 gagcagccgt cgctgccacg gcgaggtggc aggcgagcgt cgtcttgagg acgccgccct
       61 tgccggcggc gatagcgatg gtgttgagca gtcccatagt ggttcgattt caaacaatgc
      121 gatgatttag cagtcgtggg gatgaggaat cgtaggccaa cgatggcgtg ggcgttccac
      181 caccggaagc gcctgcgacg catctgcgac gcatctgcga cgaatctccg gcgaatggtg
      241 cggcgatcgt gtcgccgctc ggctcaactc ccgatatgga ctccaaccag cgccacatct
      301 ccgccgtccg tcatcgagaa aggctccgag ttcggcgcgt cggctggatg ccgaggttcg
      361 tcgttcgcgg ctcgatcgtg gtcgtcgcag tgatttctgc gctactgccg ccggcgtttg
      421 gaacggctgg cgcgacgcca ggcgtgccgg tggggcaggc tcctcagccc atgtgcgcgt
      481 cgacgaatcg gagctaccac atcacggggc tagatgacac cgagaaggtc agtggcttgt
      541 gggtgccggc gggggcgaca gtcgatttgg tggcgacggg gtcgatgacg acgagcctct
      601 actggggtct gtaccgggcg gagtacgcgt ttcatgtgct ggctcttggc ggccagttcg
      661 cgtccttcgc tgacgtcggc tggtggccgc cggcgtcggc ggaggggccg gccggcgggt
      721 ggtatccgtt cccgggcctg acgtatgggc acttcacgaa cacgacggga gcgccggttc
      781 ggtatcagct gaagaactgg ggcgcccgta cgttcgctgg tgctggagtg cgttgggagt
      841 cgatcgtcac ggtgaccggc ggcgacgggt cagctgaccc cttgtgtgcg gccctgcgcc
      901 ccgatggcgc ggctcggggt gacagcgctc cagggaacct gcgggtgagc gatccggtgg
      961 acacggcgac gggcaacctc tatcacgggt tcacggacct cgacgccact gcgtcgttgc
     1021 cggggcttgc cgttcagcgc tcctacaact cgactgacac cgatggcgca gcgttgggtt
     1081 cgggatggac cgaggcgtac ggtgccaccg cgacggactt cgtgtcgtac gcggatgtga
     1141 aactcgacga cggccgggtg gtgaggttcc cggcgaacgc tgcgggtacg gggttcaatc
     1201 gcccccagga gctggcggcg gagctttctc gcgatccgga ttcgtcgctg cgtctgacct
     1261 tcaacggggg tgtggtgtgg gagttcgaca cggccggccg gctggagaag acgaccgatg
     1321 ccgctggagt gtccgcgacg gtggtgcgcg acgggtcggg tctggtctcg acggtgacgt
     1381 cgtcggcggg taccgcgccg aaccagccga agctgtcgtt ctcctactcc ggcggcttcc
     1441 tggtgtcgat cgcggcgacc gacggccggt cggtgtcgta cggctacacg tccggtcggt
     1501 tgacgtcgtt caccgatgcc gccggcaagg tgtgggcgta cgggtatgac gctgctggtg
     1561 cattgagcac ggtgaccgat ccgaccggtg tggtgttgat gtcgacggtg tacggcagct
     1621 cgggtcgggt cgtcttgcag accacacctg acggtgggga ggagacgttc tcctacgacg
     1681 acgtcaaccg gatcacgacg cacaccgtgg tcgcgactgg tgaggtcttg aagtacgtgc
     1741 acgacacgaa cgggcgtgtc gtccagatca tcgacccgaa caacaagacg actggcaagc
     1801 aggtcggctc gttcgggttc tggaatcagt cgaccacgcg caccggagtg accaggggtt
     1861 cgacgctcga tgcccgggcg aacgtctcgg cgacgaccga cccggcttcg gggccgacct
     1921 cggcaacgta tgactcccag gatcgcccgt tgacgatcac caccgcggct tcgggcacga
     1981 cgacctacag ctacgccggg tcgggaacgt cggagatccc atcatcggag accgacgcgc
     2041 tcggaaaggt caccacccgc acgattacga acgggttggt gatgtcggag accgaccctg
     2101 acggtgtggt gaggtcctac acgtattcgc cgactcggca ggtgctgacg tcgacggtcg
     2161 gcggtgcgac cacgacgatg gagtactggc cgcaggggtg gttgaagaag gtgacgtcgc
     2221 cggaaggaag ggccacgtcc tacacgtacg acggcgaggg gcgggtgttg acggtgacgg
     2281 cgccgggcaa cgctgcgggc accaccgagg tgacgaccta cacctacgac ggagcgggtc
     2341 gcttgttgac gacgaccgat ccgacgggtg ctgtgacaac tcgtacctac gactcgctgg
     2401 ggcgcctcgc gaccgagtcg ttgcccggca agctggcgac gacgtacggg tacgacgcgc
     2461 tcggtcagct gacgacggtg accgacccga ccggtgtggt tactgaaacg cactacggag
     2521 tgttgggccg tgtggagtgg accaaggatg gggccgggcg ccagacgaac tactcgtact
     2581 tcgatgacgg tcagttgtgg aagacgacgg cgcccgatgg tggggtgacg gagaacacct
     2641 acgacagcgc aggccgattg tggaaggtga aggacccgat ggcgcgggtc acgacgacga
     2701 cgtacaacgc ggccggtcag gtttggaagg tcacggcccc tgatggcggg gtgaccgaga
     2761 ccgagtacga cgcggtgggt cgggtcaaga aggtcaccga tccgaccggg agaatctccc
     2821 agaccaccta taccgatgcc ggtcgtaccc agacgacgac cgacgctgct ggtgttgtga
     2881 ccaccctgac gtacgactcg tcgggacggg cctggaagtc ggcggatccg ctgggtaaca
     2941 cgacgatcac cgagtactgg cccgacgggt tggtgaagaa gcagacgtct ccttcaggcc
     3001 tggccacgaa tgtcgcgtac aacaatgcgg gccgccagag tgtggtgacc gacccggccg
     3061 gagtgacgtc gacgaccacg tggtcggctc gcggcgagcg gcttgccgag cagatctccg
     3121 gtcgtgggcc caagcaattc ctgttcaacc ctgatggcac gatggcgtgg tcgaaggacg
     3181 agcggggcaa ccagacgacg tatgcgtacg acggtgctgg ccgctcgacg ggccgtgtga
     3241 cacccgtcgc gacggaaggc tggggatacg tcaatggcga gatggtgtcg tacatcgctc
     3301 cgccggtggg cggtgtcgcc cgggtgacgc tgttcggccg tgatgctgcg ggtcgggtga
     3361 acgcggtggc ggacccgtcg ggtcgggcga tgaacgacac gttcaacgcc gccggcgatc
     3421 tgaccggacg agtcttcact caaggcggtt cgacgctcgg cttcacgtat ggctacgaca
     3481 gcgcgggccg gcaaaactcc gcggtgacgc ccgatggcac gttcaccaag accttcgacc
     3541 tcgcgggacg actcctgtcg gtgaacccgg tcgacaatcg atacatgtcg tactcctacg
     3601 acagcggcgg gcgtgtgtcg caggtgacga cgcccgaggg tctcgaactc gtctacggct
     3661 acgacgccgc tggtcgggtc cagaaaatct cacccaactc gacgatggtc gactggttca
     3721 acggtgccaa tgcggccggg gccgatccgt ccaagtggac tcgacaggca gttggtggtg
     3781 gctcggcagc gattgacggc aaccgactcc gtctgtcgac gacagcgacg gccgggtcgt
     3841 cgatgggtgt gacgtcgacc gctcctcaaa cggcggattc ggtgacgacg gtgtcgtttc
     3901 aagcggcatc gagtgctgtg gggaaccagt cgcggttcac ggttggtgtt cggcagaacg
     3961 cggcgaacac cgcggggtat cgggttgagt tcacgactga ctcgacgact gcgaagctga
     4021 tcaagcgcgt ggccgggacg gacacggtgt tgggtacgtt cacggcaccg acaccgggta
     4081 cggagatccg gacccagatc gaggtgtcgg gttcgacggt gcgggcgaag gtgtggccgg
     4141 catcggggtc tgctccgggt acgtggggtg tgtcggtgac tgatacgtcg atcacggcgg
     4201 cgggggcgac tcaggtgcgt accgatcggg ttgcggggtc gaacagtgtg gcgatcgaca
     4261 gttaccggca acgcaacaat caggggtcgg cgttggctcc gttcgtgacg aacacgtaca
     4321 acgctgactc ccaggtcacg acggaggcgt tcccgtcggg atcgcgggtg aacacgttca
     4381 ccgctgggcg gttgacgaag caggtgcaaa cggtgccggg cgcagaccgg accactgatg
     4441 tcacgtatga cagtgcgggt gcgtacgcaa cgacggcagt tgggggtgtg acgacgacgt
     4501 atgggtatga cgcttccggt cagttgttgt cggcgacacc gtcgacgggt gcggcgttgg
     4561 agtacaccta cgatggtgcg gggcgtcggg cgacgtcgaa ggtcggttcc gtgacgacgt
     4621 cgtacaccta caactcggct tcgcagttga cggcggtcac gccggctgct ggttcggcga
     4681 cgtcgatgac ctatgacctt gcgggtcgcc ggctcacgga gacgaccggg tctgcggtga
     4741 cgacgaacac gtacgatcag gcgggccggt tggccggtat cgccaagacc aatgctggca
     4801 cggtggtgac ctcggaggcc cgcaagtaca cccccgaagg tttgctgcgg atggacacgg
     4861 tgaccggccc gggtggcggg ttcttgcggg cgttgaagta cgactgggac acgaacaccg
     4921 gtgggctcgc tcagatggtg tcgacccttg atggtgaaac gaacttcggg ctcgttcgga
     4981 acaacggtac ctgggcggcg tcgaccaagg gtggtacgcc aacgttgttc ggcagtgatg
     5041 tgttcgggtc ggtgatcaac agcaccggcc agaccctggc gaacgcgacc ggttgggacc
     5101 cgtacggcaa cccgacgggt gcgaccgcga cgaacgctgc acggttcggg taccgggggg
     5161 agctcaccgt gctgggtgac acctacctgc gagcccggaa ctaccagaac acgaccggaa
     5221 cgttcaccac ggtcgacccc ctcgacgacg tcgctgccac gtcgacgtcg gggaaccggt
     5281 accactactc ctacaacgac ccgatcaacc ggcaagaccc gtcagggctg cggcccggag
     5341 agcccgatca cgagcaccaa gcgggccggc caacgggcag cgcgggagca tcggccgccg
     5401 ggtcgtcgga ccgtgttcgc cggtcggtgg aggacgcgat caaacgttgc ccggcgcccg
     5461 aggatcacat tcaacgtgcg accacgaagt ggtggtacga catcgacttc gcggcgagga
     5521 cgtgcacgtt gaccttcaac gggaagatgt cgaacggtct cgagcagctg atcgtcgact
     5581 atcagaccga gatcacagtc ggtctggcgg tggtcgcggt tgctgccgcc gcagttgccg
     5641 tggcggcggg tggaggggcc gtcgttctga gtgaggcggg aaaggcgtcc gttgaggtcg
     5701 tggcgggagg atctgcgctc gtcgtcgggg tagcagtcgc tgggggagtg gtcacggtcg
     5761 tggtgggagg cgtgatcgtc gtgctcagaa acctcgacgc cgccggaccg agtccgcaga
     5821 cgagcggccg aggcggtggc agcaccacga cttccacgcc atcgacctgc ggggccacag
     5881 aagacgggcc agacgaatcg gggaaaggac gaagcgccag tcctgtcggg gaggttctcc
     5941 gcgatggagc ggtcaaggtt gtcatctact cgaatgacca cggcccgccc cacgcgcatg
     6001 tgaaaggcgg tggccccgag acccgtgttg gtcagaacgg aaagccgctt aatggggatc
     6061 ccgaactcac gagggcccag cggcaggtcg ttgagaagca ccttaaggag atccgcgacg
     6121 caatccggga atacatgcgg tggtttggtg acgagtgctg acatgacgag caacagtgaa
     6181 gtcttcgaaa gtgccgttgg ccgccttgcg acggccttcg gtcacgagcg tgaacatgtt
     6241 ctcgcggatg agcacgcgcg ctaccgcctc tacctagagg cataccggca gtctccggac
     6301 cttcggaaat cggcggtcga tgcgatcgcc atggagccgg atcgtgcgat gcagcaatcg
     6361 accgtcgggc ttctgctgga tgaggccgcg acgaggtcgg cctccgatag agaccttgcg
     6421 cgatggtggg ctacatggcc agagaccatc cgacggatcg actacttacg gagacgcgag
     6481 gccgagtggg aggtcgtact ccggctcgca tgtgggggcg atctcaacct gagtgaagtc
     6541 agcgagtctt ccgactggct ccagcggaag ctcgccgcac agggcaggcg agacgtcgcg
     6601 gagtttctta gtgagaacgg tcgaactcgt cggatacgca acctcgcgtc tgctcgcctt
     6661 actggtgatc tgtgatgtgc cacaaactcg ccccttgcgg gtcgccggct cacggagacg
     6721 accggttcgg cggtgatgaa tcgccctggg cttggtggag gctctgacct gccccacacc
     6781 cggcttccgg agtcggtgac gagtcgtcag agcggggatg gtgttggggg aggttctcgt
     6841 atccggcctc aggctcgaac ttccgacgtg gaacccgcgt gaggcgggtt tcccgtcgga
     6901 cgagccgcgg acgggtgtcc ccggcggcgg tcgtaggctg cggcgatggg tcgaggccgg
     6961 agtgtcagga cgatgtcggt gcgccaccgc gacggcgtcg ccagccgcgg gcccgacgac
     7021 ctcatcgtcg aggagcccat ggagatccgc ctcgacgacc atctcgtcac gaccaccatg
     7081 cgcacgccgg gccacgactt cgagctggca gtcgggttct gccacagcga aggcctcctc
     7141 ggtggcgcgg cggtcaacgg catccgctac tgcggcacgg gcaccgcggt ggacaccgaa
     7201 ttcaacgtgg tgtcggtctc gaccggtggg tgggcccccg agcccacggc cagggtcgga
     7261 acgacgagct cgtcgtgtgg catgtgcgga tcgatggcca tcgagcagat gacggaccgt
     7321 ctcgatccga tgcagcccgt cgagttcgac tggacgttgt tggccaccgt ggccgaacgg
     7381 atcggccggc aacaggagct cttcgcccag acgggggcct cccacgccgc gggagcgttc
     7441 cgtctcgccg acggtcgtgt cgacgtggtg cgcgaggaca tcggccgcca caacgccgtc
     7501 gacaaggtgg tcggtcggct cctcctcgac gacgccctgc cggcgctccg agccgatgcc
     7561 gagccgatgg gcctgtgggt atcgggccgc gccagtttcg agatggtgca gaaggcgtgg
     7621 gcggggggat tccgtgtgct cgcatccgtg agcgctccga gctcgctggc cgtcgacctc
     7681 gccaagcagg gcggcgtgtc gctcgcggga tttgcccgtg gcggcgacct caccgtctac
     7741 gcgtaaggtc ggcgtcatgc ccgacgagga atccaccgag ccgacgatcg agcgggtgct
     7801 cgtgatcatg gcgcaccccg acgacgtcga cttcggcagc gccggcaccg tcgccacctg
     7861 gaccgacgcc ggcctcgaag tcacgtactg cctggtgacg agtggcgacg ccggcggcag
     7921 cgacctcacc cagtcctacg aggaccgagc gtcactccgc gaggcggaac agaccaacgc
     7981 cgcggcggag gtgggtgtca ccgaccttcg gttcctgcgc ctgcccgacg gtcggctcga
     8041 atacgacctc gatctgcgaa aggccatcac ccgcgtcatc cgccaggtgc gcccgcaccg
     8101 cgtcctgacc cagtcgagcg aacgcaacgt cgaccgcatc tacgcgagcc accccgacca
     8161 cctcgcggtg gggcaagcgg cgatcgccgc ggtgtatccc gacgcccgca atccgtttgc
     8221 cttccccgag ctcctcgcga acgaggaact ggagccgtgg accgtgccgg aggtgtggac
     8281 ccagggcggc ccggagatcc ccgaaccggc gcgggtggtc gtcgacatca ccgacacgtt
     8341 cgaccgcaag gtcgcggcgc tccgtgccca cgtcagccag accggcggcc gcgagggcct
     8401 tgcggacttc ctccgggaat gggcggtcga caacgctcgg atcggagggc tgcccgaggg
     8461 ccggctcgcc gaggtctatc ggcggatcga cacgcggtag ttcagccgcc cgtacacggc
     8521 ccctcgccgc agacgtcgat gagggtgccg tcgttcacga agctcacgag gagcgcgagg
     8581 gcggacgtct cgtctccgag gtcgccgtgg ggatcgtcgc cgccgcgatt cggtgtgttc
     8641 gtgtccggcg gcgtcggcgt gccgaagtcc cacacgaaga gcgccgagcc cgtcgtcggc
     8701 agttcgggca acggggcgat gccgtagaac gggtcgactg cggacgaccg accggcggcc
     8761 agggtgggtt cgcggcggcg aagtcccaac gttcgggcga gcttctgcgt gctcacgttg
     8821 gtcacctggt ggtcgccgaa ggcctcgagg gcgaggaccg gcttcgccgc gatcccctcg
     8881 tacggatcgg tcgtcaggtg ctgggcgtac ccggcgttct cgccgcggtc ccacaactgc
     8941 tccgcgagtt cgagcgtgag gagctggtcg agttcgccgg ggtacgcggc ctcgaaggtc
     9001 ccgatgaact cgtcgaagtc gacgctgcgt cggagcaaca ggttgtaccc gagcccgccg
     9061 acggcgagca cgaccttgtc ccagtcgtcg gtgaccgtgg acgcgacgcc gccgaggatg
     9121 ccgccctggc tcgccccgag gaacgcgagc ttgctggtgt cgatgacggg cgcgccgtcc
     9181 gtcgagaacg cgccgtccgt tgcgaacccg tcagcgctgc gcagcagccg acccagtacg
     9241 acgaaggcga gctggccctg ctgcatccgg tccggttgcg tccggaatcc gctgaagtcc
     9301 cggaactcgc cgacgatcgt cgggatgtcg gcggcgctca tgcccaggta gtcggtggca
     9361 cacatgccga tgttggcgat cgccgcggtg ccgccgatgc cgagcacctc cgaacggctg
     9421 ccgagcaggc cgtggccgta gacgatcatc ggcgccgggt cggcggccgt tgccccggcc
     9481 ggcacggtgc acgtgaagtc ggcggacatc gtcccgttgc gagcggggat gccatcgggg
     9541 tcgttgtcgt tgttcaacac acttcccggt ccgccgtcgc cggccaggta ccggggcatc
     9601 tcgaacgtgc cactcacgat tcgcgccgcg cccgccgtct cgttcgacgt gacggtgaac
     9661 ggcggcgccc catctccgaa ctctgagagt gtctcggcgg ccattgctcg gaggcggccc
     9721 gacagcgagt cggacgaagc gaccgtgaac gaccacgcga tgtcgaggtc atcggtcgtc
     9781 gccccggcgg ttcccagcgc gtccagccac tcctgctgtt cctcgtcggg atcgtccacc
     9841 agctcgctga acgctccggt cggttcgacc tcggtgccgt cggtgcgtac gaggccgcgc
     9901 agcccgaccg cgtagcggtg cgcctcggtc aacgaggtcg cggggacgat gatcagggcc
     9961 tgctggtcgg gatcctcggc attcgcgtcg agttcggccc acgccgcaac ccggtcgccg
    10021 gtcgtgacgt cgacgagcac gagccccgag tcgtcgtcga gcgagcgaga gaggtcggtt
    10081 accggaggga gcttcgacgc cgacacgtcc agcgactcga cgtgcaccag caggttggac
    10141 gccggactga agccgtcgtt gcggttccat tcggtggggt cgatgtggac accgtcggcg
    10201 ttgaccggcg taccgtcgac cggcaggtcg acgcgccggc cggtcggggt gctcgggtcg
    10261 gcacgggtga accggtcgtt gggccagggc aacaggcagg cggtcgggtc ggtgtcgtcg
    10321 caggttgcgg tgccaggcgg ttcgctcacc ggtgccggac gagcggtcgt cgccgcacgg
    10381 tccgatgcgg caccggcacg gtcgtcatca gccccgtcgt cggacgagca cccggcgagc
    10441 agggcgaccg tagtcagcag gatgatgaag ggacgcatcc gggtcgacat ccggccgacc
    10501 ttaggcacgg tgtcgaacgc cacgacggct cgaatgcccc atcggccggg acagccctaa
    10561 ccttgcggca tgaacgcctc cactccgcag gtcggcctgg ccgtcgccca cttcttctgg
    10621 tccacgtcgg tgctcgccga tgccgacgcc gtgctcgacg tcatcgcgaa ggccgaggcc
    10681 gacggcgatc aggtcgtcac cgcctcgatg ctgggtcaca agtccgacct ctgcacgatg
    10741 gtcctcagca ccgatcagtg gcggctccgg cgcttccaga cggcgatggt cgaggcgggc
    10801 ttcgaactcg tcgacagcta cctgtcgctc accgagatgt cggagtacgc gcaaggcctc
    10861 cccgaggaga tgaagcaggc gcgcctgttc ccccagctcc cgcccgaagg caagcccgcg
    10921 ttctgcttct accccatgtc caagcgccgc aacgtcgggc agaactggtt cacgctgccg
    10981 tacgacgagc gcaaggcgct catgatggaa cacggtgcct ccggccggaa gttcgccggg
    11041 cgcgtcgtgc aggtcatcac cggctcgacg ggcatcgacg acttcgagtg gggtgtgacg
    11101 ctgttctgtc agcaccccga cgatgtcaaa gaggtcgtct acacgatgcg gtacgacgag
    11161 gcctccgcga cctacgccga gttcggctcg ttctacgtgg gcatggtggc gtcgccggtc
    11221 gaggtgctgt cgagcctcgg cgtcaccaac gcggactgat cgccctccgg cgaatcaccc
    11281 tccgacgagc tgcgccagct cgtcggtcat gtcggcggcc gtgccgtacg tcgcgatcgt
    11341 ggacgggccc ttgcccttcg cgaccagacc tctgacgacc gacgcggccg ccggcgccga
    11401 cgtgacgagc accagcgcct gggggtcgga gtcgatcagc tgttgagcga ccgctgctcc
    11461 gtcggggtcg tcgccgacct ccacggtcgc ggtgacggtg cccgagtcgg cgttgatggc
    11521 cgcagcgatg tccgccgcga gctcctggtt gtcgtctccg gtaccggctg cgatcgcgac
    11581 ccgcgtgtac ccgtcgttgg tgatgaggtt gccgagcacc tgcccctcga ggtcggtggg
    11641 gggcgacagc cggaagaaca ggccgcggtc gtcggcgacc gacaaggccc gggccgtgtc
    11701 ggtcggcgag aagaggatga tgccggcgtc ggtgagctcg gggatcgcaa cactcgtgat
    11761 gccgtagacg gttcctccga tgacggcgtc caccgggcgg tcgatgagcc gtcgtaccgc
    11821 gtcggtggtg gccgacgacg agccgtcccc cgattcgtcg ggcacgagca ccacgggttc
    11881 gccgagtaca ccgccctcgg cgttgacctc gtcgacggcg accttggcac cggcgagtgc
    11941 ggcacgggcg gcggcgccat cgggacccat gaccggcagc aacgtgccga tctcgaggac
    12001 cccattgccg atcggtccgt agatcgggtc agccccggcc gggcgatccg ccagcggccg
    12061 ggactgcacc gtgacctggt cgactgcccg gagggcgccg gtgccggtga actccacgac
    12121 cgcaaagccg gcttcgcccg gatcgccgtt ggcgagcaga ttgatgttgc cgctgacgcc
    12181 gtcgtagtcg aagtcggtgt tgtcgagcgc gaactcgcga caggcgtgga acccgagaca
    12241 ggtccgacca ccctcggtgc tgctgatcac ctcgtcggcg agccgccctg gtgaatcgct
    12301 tcgtgccgcc tccgccgaga tggccaggac catcacgatg tcgtagccct ctgccgcacc
    12361 cgtgaagtcg tcgaccgtgc cgtcgacggc gcggacgcga tcgcggaagt cctcggtgat
    12421 gcggatgccg ggcgccgcac ccttcatgcc cttgaggggg ctggaccccg acgtgatcgg
    12481 gccggtgcgt ccgccgcccg gatcgatggt ggcagcgtcg gttccctcgc cgaattcggt
    12541 ggccgccgtg gtcgtcgtca cggcggcgct cgacgactcg tcgagcttgg cgttggtgca
    12601 cgaggccgtc gcggcgccca ccaacgcgat cgtcaggggt accagggcgc tgcggaggag
    12661 cggtctcgcg gcgcgcgggg ccgaggcggt cgggggcata tcggggacca ctgtagaagc
    12721 gctacggtcc gggagcgatc gtcggccgga gggggctctc agtgaccgaa ctgttccaac
    12781 cactcgcgcg tgacctcggc gacgccccgg cgctcatcga cgaggcgggt accacttcct
    12841 ggcgccagct ggacgaacgg gcgaaccgca tcatcggtgc gctccgtgcc cgaggcgtcg
    12901 gcgacggagc gaccattgcg gtgatggtgg gcaaccgtcg cgagttcttc gagatcttcg
    12961 ccgcggtgtg ccacgcgagc tgggtcgcgg tgccggtcaa ctggcactgg gtgggcgccg
    13021 agctcgcgta cgtgatcggg aactccggag catcggcgat gatcgtggac gaggcgtacc
    13081 tcgacgtcgc ccgcgatgcc cgtgggcgag aggaatcggc gaactgcgaa cactggatcg
    13141 cgctcggcgg cgacgtcccc gacgacttcg agtccttcga gtcgttgctc gccgcggcgt
    13201 cgcccgacga gccggatggc caggcgacgg gaggaccgat gttctacacg tcgggaacga
    13261 ccggattccc gaagggcgtt cgctccaccc tgtccaccac cggcctgccc gcctcgatgt
    13321 ggacgctcat ctcgggctcc atgctccaga tgctcggcgt gccggcagac ggcgtcacgc
    13381 tgctcgacgg gccggcgtac cactcggccc aatgggtgtt ctcgatgttc ccgctgttcg
    13441 gcggatcgac ggtggtcatg cggcatcggt tcgacccggt cgagacgctg gccgccatcg
    13501 atcgccacgg cgtcacgaac ctccacctcg tacccaccca gttcgttcgc ctccagaagc
    13561 tcgacgacca ccagcgggcg acgttctccg gcgagtcgct gcaggtggtg taccacggag
    13621 ccgccccgtg cccgcccgac gtgaagaccg cgatgctcga ctggtggggc gacgtcgtgc
    13681 acgagtacta cggcggcacc gaggggggct tcctcaccac gatcggcggc gcggagtggc
    13741 gggcgcgtcc tgcgagcctc gggcgcccca ccgatctgac cgagctccgt gtggtcgacg
    13801 acaacggcgc cgacgtgccc gccggcacct ccggtcagat ctacttccgt tcccgtatcg
    13861 gctccgactt cagctaccac gaggatcccg acaagaccac cgccgctcac ctcgacggct
    13921 ggggaaccct tggcgacatc gggtacttcg acgacgaggg ctacctcttc atgtcggacc
    13981 gtcgaatcga catgatcatc tcgggcgggg tcaacatcta ccccgccgag atcgaggccg
    14041 cgctggtcgc ccatcccctc gtcgccgatg cggcggtgtt cggcatcccc gacgacgaga
    14101 tgggcgaaca ggtgaaggcg gtcgtcgagc tggtcgacgg tgcgatcgcc gacgccgcgc
    14161 tgaccgacga gctgatcgcc cactgccgag gccgccttgc cggctacaag gcgccgcgca
    14221 gcatcgacgt caccgaccgg ctgccccgac aaccgacggg aaagctctac aagcgcctcc
    14281 tccgggaccc gtactggacc gacgtcggcc ggagcatctg agaccgaccc agggttccga
    14341 ccgtgcggca ccggccggca cggccatact ggcgcgatga ccgagcctgc cgaagcccag
    14401 cccgaaaccc ttgtcgggat ctcgttcccc gacatcttcc gcgcccagga gttcctgacc
    14461 gccgccacac gcatggcgtc acgcggtgaa ctcgccctca aggatgccgt cacggtcgtg
    14521 aagaactccg acggaaagac cgtggttcac gagacggtcg acctgcagac gggccagtcg
    14581 gcgatgtcgg gcgccctgtg ggccggtctc atcggcctga tcgtgggtgg gcccgtgggc
    14641 tgggttgccg gcctcgccgt cggcgccggg gccggtgcgg ccaccgcgaa gctcgtcgat
    14701 ctcgggctcg ccgacgagtg ggtcgagtgg ttccgtgagg tcgtgcaacc cgacaccgca
    14761 acggtggcgc tgctcgtcac cgaactcaac cgcgatgcgc tcgtgaccga agcgtcgcgg
    14821 ttcaccggcg ccgagctggt ctacaccaac ctcgacccct ccacgatgga ccgggtcaag
    14881 caggccctcg gcgatcacac ggcagcgccc gccgatgccg aagcgcctcc cacgaccgcc
    14941 ccgtccgact gacgctcgaa cgccgcggcg tcgtcaggct ctcggatcga acgaggactc
    15001 ctcggccagc agcgtgaaca gctcggcttc gcgctcggtg agttctttcg gtaccacgat
    15061 ggtcacgacg gcgaacagat cgcccgccgc cccctggggg ttggggaggc cccgcccctt
    15121 gaggcgaagg cgtcggccgc tcgacgtccc ggctgggacc ttgatcgtcg ctgccccacc
    15181 gggggtatcg acgctgacct tggcgcccaa cgcagcttcc cacggggcga cggggacctt
    15241 caccgacagg tcgcgaccgt cgacggtgta gcggccgtgt ccggcgaagc ggaccgtgag
    15301 gaacaggtcg ccggccggcc ctccaccagc gccgcggacg cgaatgcgct ggccctcgac
    15361 aatgcccacg gggacgttga cgttgaccga gcgggtgccg cgtggacccg acagcgtgat
    15421 cgaacgctcg ccaccgtggt acgactcctc gatcgacagc tcgatctcga cctgctgatc
    15481 ggcacccggt cgcgggccgg tcgcccggcg accgccgccg aacaagccgc cgaggaggtc
    15541 ctcgatgttg ggggtggccc cgccgccgaa cccgaagtcc cccgccatcc ggccggcgcg
    15601 gttccacatg ctcggatcag tgccgtcggg aaccttgcgg aagtcctcgc cgaacgcgtc
    15661 gtacttcttg cgcatctcgg gatcggagag cacctcgttg gcctccgaga tcgccttgaa
    15721 ccggtcctcg gcgcccggct cggtgttgac gtcggggtgc cacttgcggg caagtttgcg
    15781 gtaggcgcgc tggatctcgt cggctgatgc cgtgcgcttg acccccagga tctcgtagta
    15841 gttctgcgcg gccatgtgca caacgtatcg accgcggacg acctcgacgg aagggtcagc
    15901 cggtggcgga gctgtcgatg cgttcccaga gcgggaatcg gatgtgtgcg atctcctggt
    15961 ggtcgacgat gcggccgttg acggcgaggt gctgcagcgc gtcattggcc ttcgccgggc
    16021 cgccgagcaa cgccatgaag gtgggcaccg ggaggagcaa ctcgccctcg gcgtcgccct
    16081 cccggaggat ggcgtcgacc tcgaggacgg gacgctcagg tcggtcgagc gagagcgcgt
    16141 ggcactggtg gacgacccgt tcgaacacgg cgatcgcctg gattcgcatc gcgagactct
    16201 agcgccgcgg gcccgatcat gaaggatgtg tcaggtttcc tccgaccggc cggttgtcgg
    16261 ctaggcaggg ggcatgagtg acgagagcga cgcgcgcctg atggccgaga tctcggcgcg
    16321 actggtcgag gcgagcgccc gagccctcgg cccgtgggtc gagaccgccg tggcgcagcg
    16381 ggccggcgac cgcgccgccg aactggcgtc ggcggcgacg gcggcagggg agcggtgcgc
    16441 cgaggaggtc ccggcgacga tgaccgccct cctcgcgctc gacatcgacg cgcaacgctc
    16501 caccccgctc ggcatcctcc ggtcggcgtg ccgctaccca gctgccgtgt tggctgcggc
    16561 cggcgttccg gctcctgccc gggacccctt cgacgagcga gccaatcccg acgatcgcta
    16621 cgaggtgggt ccggcgacgt gggcggacct cggtgaggag gtcggtgaac tgggcatcgc
    16681 ctggggggcg gccaaggctc acctgcacaa gcgtcggcac ctgggacgct gaccgacggg
    16741 tcgtccgccc gggcccgtaa caaccacgaa acccggcctc acatacactc ctgagcgcgc
    16801 accgaaccag aaagcgactc gccatgaagc tcgtcaccgc catcatcaag ccgttcaagc
    16861 tcgacgacgt gaagtccgcc ctcaaggcca tcaacgtggt cggcctcacg gccaccgagg
    16921 tgcaggggtt cggtcgtcag ggcgggcaca ccgagacgta ccggggcgcc gagtacaaga
    16981 tcgacttcgt gccgaaggtc cgtctcgagg cggtcgttgc cgatgacgac gtcaacgcgg
    17041 tggtcgacgc catcgtcggc tcggcgcgga ccgacaagat cggggacggc aaggtgtggg
    17101 tcaccaccgt cgaccagctc gtccgaatcc gaaccggaga gcgcgacaac gacgccatct
    17161 gatgccgccg gtactcgacc gatcgtccgt cctcgacaac cccgacctac gcggtcggcc
    17221 gttcgtcgac gcctacaccc gtctcgtcga cacgtggatc gtcgagctct acgacgagct
    17281 cttcggtgaa cggtccggcg tcgcgctcgt tgccaccggc ggccatggcc gtggcgagct
    17341 cgcaccccag agcgacctcg acctcctgct cctccacgac ggttcgatcg acctcggcac
    17401 cgcccagcgg ctctggtacc cgatgtggga cgccaagttg aaggtcggcc actccgtccg
    17461 aacggtggcc gagaccctcg cgctcgcctc cgacgacctc ccgaccgcca cctcgctcct
    17521 gagcgtccgg cgcctggccg gtgatgcgtc gctcgccacc gaccttgccg tcgacagcgc
    17581 ggcccagtgg cgcaagcagt cgaagcgggt cctggcgtcg ctcatggaga ccgtgacctc
    17641 ccggcacgag acgaccggag aggtcgcggc cgcgctcgag cccgacctca aggaaggccg
    17701 cggcggtctc cgcgacgtcc acgcgttgtc gtggatcctc gccgccgcgc ccgacctcga
    17761 catcccccgt cccgagtcgc tcgccggcga gtacgacacc ctgctcaacg cccgggtcga
    17821 gctgcaccgc gtcaccgggc gcgccggcga cgtcctccag ctccatgacc aggacggggt
    17881 cgccgaccga ctcggcatcg agaacgccga cgtgttgatg accgaggtgg cgacggcggc
    17941 gcggcgcatc gcgtggtcga gcgacgaggc gtggttcgtc gtgcggggct cgctgagcgg
    18001 cggcatcttc aagcggtcgc ctcggcgcca agacctcggc cacggagtgg agctctgcgg
    18061 agaacgggtc cacctcgccg aaggggccgt caccgacgag ttgagcgtgc tgacggtcgc
    18121 cgtcgcggcc gcgaagcacc gtgcccgaat cggtcgcacg accctcgtca cgttgcagtc
    18181 ggcgccgcca ctgcccgagc cctggcccgc ggcagcccgg gcactgttcg tcgagctgct
    18241 ccggtgtggt cccgcagcca tctctgtcat cgagacgctc gacctcaacg atctgtggct
    18301 cccgatggtt cccgaatggg agcccaaccg gagccggccg caacgcaacg cctaccaccg
    18361 gttcaccgtg gatcggcatt cgtgggagtg cgtggccctc gccgccggcc tgaccgatcg
    18421 agtgactcgc cccgacctcc tggtcatggg agcgctgctg cacgacatcg gcaagggcta
    18481 tccgggtcag cactcgatcg tcggcatgga actcggtgcg gcggtcatgc ggcgcatggg
    18541 gtaccaggcc gatgacatcg acactgtggt cctgatgatc gagcagcacc tgttgttgcc
    18601 cgacgttgcg acccgccgcg acctcgacga cgccacgacg atcctgctgg tggcggcgcg
    18661 ggtcgagacc acggaacggc tccgcctcct ggcggcgctc gccgaggccg actccatcgc
    18721 gaccgggccc tcggcatgga gcccctggaa ggccggcctc ctcgccacgt tggtcgaacg
    18781 ggtcgcgttc gtgaccgatg gcggtgacgt cgaacaggtg atcaccgcca gcccagcgac
    18841 gctggcccaa ctcgagttgg tggagcgcgg ggccgccggt ggccgcccgg ccatcgacgc
    18901 ccacgacgat cgactcacgc tcgtgtgttc cgaccggccc gggctgttca gccggatcgc
    18961 cggagtgctc gccctcaacg gcctcgacgt gctcgaggcg tcagcacaca cgtcgggcga
    19021 cctcgccatc gaggagtttc gtgtggtgtc ggccttcggc ggcgagatcc actggagcaa
    19081 ggtcgagcgt gacctggaac gggccctgct cggccggatc gccctcgaac cccgtctggc
    19141 cgaacgggcc cggagctacc accgtcgtcg caacaccggc cgaggactcg agcccaacgt
    19201 ccgggtcctg gagaacgagg cgtcggacgg ttcgacggtg gtcgaggtgg tcggccccga
    19261 cggcgtcggc ctgctgtacg cgttgacccg ggcccttgcc gacctcgacc tcgacatcct
    19321 ccgcgccaag atcgcgacca tgggcagcga cgtggtcgac tcgttctacg tccgcgaccg
    19381 gtccggcgcg ccgatcaccg acgcggacga cggagccgag atccgtaccg ccctcctcca
    19441 cgtgctgaag gccaacgcgt cgtgaccgac gttcccgctc ccgacgacct gacgcccgac
    19501 gactcgatca ccgaggccga cctcctgcgc tgggcgcacg cgttgtccgg catcgcccga
    19561 accggactgg gcttcaccga gagccgctac gaacgggagc gctacgagga ggtcctggcc
    19621 gtcgccgccg acatcctcgc agcgtcgtcg cggagccatg atcgagcggc gctggtcgac
    19681 gagtggctga agatggtcgg cgtcggcatc gccgggtacg tgacgccgaa ggttgccatc
    19741 ggcgcggtcg tgggcaacga cgccggcgag atcctcctca tccagcgtgc cgactcggga
    19801 atctggctct acccgacggg atgggccgac atcggctatt ccccggcgga ggtggcgctg
    19861 aaggaggtgc acgaggagac cggcatcgtc gctgaggtcc gggggttgct cggggtcttc
    19921 gacgggcaac gccgcgcctt cagccggata ccgctctact ccctcgtctt ccactgccac
    19981 gcggtcgggg gtgaactcga gccccatcca ctcgagtgtc tcgatgtcgg ctggttcgcc
    20041 cgcgacgcac tcccggagcc gctggcgagc ggttcgctgt gggtgcaggc ggcgttcgac
    20101 gcgatcgacg gcaaggtcgt ccccagcttc ttcgatgcgc ctcgcggtga ggtctggaac
    20161 caccccgcct gaccggcgac cgttccggcg ctaccgctgc tcgcgtagga acttctcgac
    20221 ctcggacacc agctcgtccg cggtgtcgcc cgcaatctcg tcgcggtcgt aggtctcctc
    20281 gaggtgggcg acgtacgccg ccgtctcctc gtcctcgtcg accaggtcgg agatctggcg
    20341 ttcgtactcg gcaaccgcga gcggaaggtc gctgtcggga accgtcgtgc cgaggatcga
    20401 acacactcgc tcgatgagcg ccagcgcggc cttgggtgag ggtgcagccg ggacgtagct
    20461 cggcacggcg gcccacaacg aggccgagtt cactccccgg ctgaagcaca actcgtgcag
    20521 gacgcccacc acgccggtcg ggccttcgta acgggaggcc tcgaggtgga gggccgctgc
    20581 caccttcgga tcgtgcgccg tgccgaacac cgacgtcggg cgggtgtggg cgacgtcggc
    20641 gaggagcgcg ccgagcgtga cgatcaggcg ggcgttcaca gcagcggcga cgtcgaggat
    20701 ctgctcgcag aacgtccgcc agcgaagttg cggctcgatc cctcgcaaca cgatgaccgc
    20761 cggcgatccg gccggctcgg cgaagaagaa ctcgttctcg ggccacaaga tgcgacggga
    20821 cccgtcgtcg tcgaactgca ccatcggccg caccgcggtg aagtcgtaga actcctcggg
    20881 gtcgatgtcg acgaatgcga cggcccccca cgcctcgcgg aggcgacgca ccgcctccgt
    20941 ggcggcctcg ccggcatcgt tccacccctc gaacgcggcg acgatgatcg gctggttcaa
    21001 cgacggttga ctcgaccagc ggagagaact cacgtcctca aacgctatcg acccggtccg
    21061 cgtttccgtc ggccggagtg accgacatcg gccggcgttg atcgtcgcgg tgcacccgta
    21121 ggctggtccg atggctctcg atcccaccgt gaccgttcgc gaacttaccg acgccgatga
    21181 catcaccgac gccgtcgtcg acgcgttcgc ccgtctgatc cctcagctgt cgtcctcgtc
    21241 cccggccccc agccgcgacg aactgatcgc catggccacc gccgacgcgg accacctgct
    21301 cgtcgcggaa gacgtcgatg gtgccatcct cggttcgatg acactcatcg tgttccgcat
    21361 ccccaccggg gttcgtgcct ggatcgagga cgtggtcgtc gacgagtccg cccggggccg
    21421 cggcgtcggt gaggtgctca accgcaccgc gctcgacctc gcgtacggtc ggggcgccaa
    21481 gaccgtcgac ctcacctcgc ggccgagccg cgaagccgcc aatcggctct atcagaagct
    21541 cggcttcgag ctccgcaaca ccaatctcta ccgccacaac ccgctggctt gatgccctcg
    21601 gccacccggc cgtcacgccc cgtcgatccg taggatctcg ccgctcaggt tgagcacgta
    21661 gacgtcgccg gcggcgtcct cagcgaagct cgacacctgc gacatcgaat ctcctgtcgt
    21721 ggggatcacc gcctgctggt ccaggcggcc gtcggtgacg acgaggccgc ggagagtcgc
    21781 ggcggcgaag tcggagaaca ggtagacgcc gttgagctcg ggcacccggg tgccgcggta
    21841 cacgaacccg cccgtgatcg acgctccctc gtcgtgggag tactcgaaga tcgggtcgac
    21901 catggcgacg gaccggtcgc cggtcttgcc ggtgtcgtgc gcgccttccc ggaggctcca
    21961 cccgaggttg gcgccccgcc cggcgccctc cgatgccggg aggacgtcga cctcctccca
    22021 gtcggcggcg ccgacatctg cgatccacag gtcgcccgtc gcccggtcga agctgaaccg
    22081 ccacgggttc cgaaggccga gggaccagat ctcgttgcgg acgccggcct gaccgacgaa
    22141 cggattgtcg tcgggaacgg tgtacccgcc gtcggagatc gacgcgccca cgtcgatgcg
    22201 gagcagcttc gccagcagcg tcccgaggtc ctgcccgttg tcgtcggggt cgccctgcga
    22261 accgccgtcg ccgaacccga cgtagaggta cccgtcgggc ccgaaggtga tgttgccgcc
    22321 gttgtggttg gcgaagggct gcggcaaccg cagcacttcg cgacgcgacc cgacgtcggc
    22381 gacgtcgccc gccatggcgt aggcagcgat cacgctggcc ccgtcgtcgg agccgtcggt
    22441 gtagctgacg aacaggacgg cgccgtcggg cgagaacgcc agtccgagca gtccgcgctc
    22501 gccccccagg tcggccacgt ccgcgacgag atcgaggacg gcgccctcgg ccgtcgtgaa
    22561 cacctcaccg tcgcgtacga ggcgccgcac ggtgccggca cgctccccga cgaacaggtc
    22621 gtcggacccc ggacggggaa ccatcgccgt cggcgactcg acggcggcga cctcggtcag
    22681 gcggacggtg agccggctca ggtcggcagg cgtcgtcggc gtcggggcgg tcgtcgtggc
    22741 cgcagggccg gtcgtcgacg atcgggcagg ggacacggcg gcggtcggca cggtcgtgtc
    22801 gtcgctgccc tcgtcgttgg agcaccccgc gagcacgagg atcggcacgg cgaggggtgc
    22861 gagggcgcgg cgaagtggca tgagcgccat tctcgccgtc ggggaggctg tccggtcgtc
    22921 agcgcgccca gcgggcggga cggatcacgc cctcctgcac caccgacgcg gcgagcgcac
    22981 cctcgcgggt gtagatgagc ccgcgcccga gaccgcggcc cccggaggcc gacggcgtgt
    23041 cctgggcgta gagcaaccat tcatcggctc ggaacgggcg gtggaaccac atggcgtggt
    23101 cgaggctggc catgaacacc tcgccgaggc tggccgacag gccgtgcggc cgcagcgacg
    23161 tgtcgaggac cgagatgtcg gaggcgtacg ccacgacgca ctgatggagc accggatcgt
    23221 cgggaagctt gccgttggcg cggaaccaca tgttctcgaa catcgggccc ggcgtgtgat
    23281 cctcgggcga tcgccagtcg ctgtagcggg tgtcgatcga tcgcggccgg tcgtaccagt
    23341 cgccgagctt gtccttccac ggcgccatgc gttcgcggaa gtcggggagc gactcgggat
    23401 cgggctggcc ctcgggcatc ggcatctggt gctcgtagcc ctcctcgtgt cgttggaacg
    23461 acgccgtgag gccgaagatc ggtcggccgt gctggatggc cttgacccgt cgggtgatga
    23521 agctcctcga gtcgcggatg cggtcgacct cgtagaggat cggcgacgac gggtcgccgc
    23581 cgtagaggaa gtaggcatgc aacgagtgca ccgtcatgcc ctcgacggtc cgggatgcgg
    23641 cgacgagcgc ctgccccgcc acctggccgc cgaaggtgcg ctggcggtcc tcgtcggggc
    23701 tctcgccgcg gaagatgttg acctcgatcg gctcgaggtc gagcagcgtg atgagcgagt
    23761 cgacagcggc ctgatccatc gggctattgt gccagccgtc cggaggcatc cgtgccggtg
    23821 ccccgggtgc tgaattcggt cggccccggc ccgaccgata cgatcgctgc gtgaaactga
    23881 cgcccagtgg cctcctcgaa cacgccaaga gcgacgaggg gcgcaagcag ctccgctacg
    23941 cgggcatctc cgtcgtcttc gtgccgctgg gtcaggcctt cgtacagatc ctcaagtggg
    24001 tcttcgacgt tcacgaggtg ctcgccgtcc tcatctcggc gtgcatcctg acgcttccca
    24061 actacctcgc caacaagcac atcgtgtggc ggcacaagaa caaggacaac caggcgaccg
    24121 agatcaccgt cttctggttg gcagcggtgt tgggcacggc gttcgccatg gggttcgtct
    24181 tcctggcagc gaagatcgtc cccgaggaca agggtgaggt cgcccacgcc attgccatct
    24241 tcgcggcgca gctgctcggc tacggcatcg tgtgggtggc gcgctacctc ttcctcgacc
    24301 ggctcatctt caaggccacc caccacggtg aggaaccctc gcccgaactg ctcgacgacc
    24361 tgcaccgcga cttccccgtc tgatgaccgg cgtgacgatg gatcccgacc ttcgcgcccg
    24421 agccgaagcc gcccgcggct tcatgccacc cgacgagggg ctcgccctgt tcgctgccgg
    24481 caccgacgca tgccgtcgcg tccccgatgc tccgctcctc gagatcggca gctactgcgg
    24541 caagtccggc atctacctgg gtgccgctgc agctgacgcc gacgtggtgc tgttcgccgt
    24601 cgaccaccac cgcgggtccg aggagaacca gccggggtgg gagtggcacg aacccgacct
    24661 cgtcgatccc gcagtgggcc gcatggacac gttgccgctg ttccgccgga cgatcttcga
    24721 cgccggcctc gaacgccacg tggtcgccac ggtcgctgac gctccgacgc tggggcggtt
    24781 ctggaccaca ccgctcgctc tgctgttcat cgacggcggt cacggtgccg agccggccca
    24841 ccgcgactac gagcagtgga cgccgttcgt ggcgccgtcg ggcatcttgg cgattcacga
    24901 cgtgttcccc gaccccgccg acggcggtcg cccgccgttc gagatctacg aacgggcgct
    24961 ggaatcgggc aacttcgccg aactcggcgc caccggctcg ctccgggtgc tcgaacgcat
    25021 cgacaacggt cgggtacacc gccccgaggc gaaggccttc tgacccgctg ggccgatcga
    25081 cctcagcccg gcttgtgggt ccggttgccc gccttcttgc gcgactcccg gtagacgagc
    25141 agcgcgatcg ccgccaggcc gagcagtaca gcgatcatca cgacgtactg cgtcggctgg
    25201 ctcgtcgaat tgggttcggg gatgatcgac ggcgagttga tcggctgttc gtcgatcggg
    25261 acgagcgtcg tcgtcggggc ttgcgctccc gccacggtcg cggtcatgag gacaccggcg
    25321 acgaccagca tcagcgacgt ggccacgacg gcgacgcgac gcacgtcagt cacccgaccg
    25381 gtcggcgacc gtgtcgtcgg tgtgggtgtc gatcagcggc gggaaccggt cgtgatcgac
    25441 gaagaggtgc gaggccgggc ttgcaccggc gagcgcgtcc gcgcccaaca ccactgctgc
    25501 cgccgtgtag gtcgactgct cgcccccggg gaagtgcacc tcgtcggggt acacgatgcc
    25561 cgtccagtag cggccgtcgt cggtgcggtg tcgttgcgcc caccggaaca gatcgtgggc
    25621 ggcgtcggtc tggccgaccg acaacatcgc catcatgcac tcacacgtct cggccgccgt
    25681 gacccacggg cggtcggaca cacaccgcac gcccttgccg tccatgatga acgcgtcgcg
    25741 ccgctcgtcg agccgctcgc ggccggcgtc gccaccgatg acgcccgcca ggacggggta
    25801 gtaccagtcc atcgcccacc ggtgcttggg agcgaacgcg tcgggctcgt cgcggatgac
    25861 gcgtgcgagc cgggcagcgc tgagttccca gtcggggcgg ccgtggccga gttcgtcggc
    25921 gatggcgatc gcgcaccgca ggctgtggca gatcgacgac gagccggtga gcagggcgaa
    25981 cgtccacggg gtgccgtcgg cgtgacgggc ccagaggatc tcgccgcggg gggtctgtag
    26041 gtcgaggacg aacccgaccg cagcgtcgac cacgggccac atggtctcga ggaatccgcg
    26101 atcgcgggtg aggagccagt ggtgccagac cccggcggcg acgtaggcga ccacgttggc
    26161 atcgagcttg tcctgctcga ccccgtcacc gatgtagtac tggtgccacg cgccgtcggt
    26221 ccgttgggtc gacacgagcc agtcgaaccc gcgttccgcc tcggcgacac gaccacccag
    26281 cgcgagcgcc atgagggcct cggtgtggtt ccacggatcg gcgtgcccgc cgtcgtacca
    26341 cggcaccatc ccgctcggca gctgcaccga ggcgatgtag tcgaccgtct cgcccagctg
    26401 gacggccgtc acgatgccgt cgacgtcatg cagccacact ggtgctcctc cgatgcgcgc
    26461 ccgccgctgc agtcggcttg gtgccgtaga cgacgacgct cttgccgatg accgggttga
    26521 ggacccgctc ggcgaacctc gtgacgaacg gtgccttcac catgtcccac accagcaggt
    26581 cgtggtactt cttcacgagc gggtgggtgt cgttcgtggg gctgatcgcg cagcgcagcc
    26641 accagtaggg ggagtgcaac gcatgggccc ggtgcgactc gccggtctcg atgccgaccg
    26701 ccgccatccg gtgccggagg agatcctcgg tgtagatccg cacgtggccg ccctgggaca
    26761 gcggggcgtg gtactcggcg gacagcgtcc agcagatctt ctcgggcagc caggccggca
    26821 cggtgacggc gatgcggccg ccgggcttga gcacccggta cagctcggcc atggcccccg
    26881 catcatcctg caggtgctcc atgacctccg agcagatgat gcggtcgaac gtgtcgtcgg
    26941 cgaagggaag gcagagggcg tcgccccgga ccgcctgggc ctcgttcgcc tcgggcacct
    27001 cgccggcgtc ccgcatggcc cacaggagat cgcggacccc ggccagctca tcgaacgagt
    27061 agtcgagcgc gatgactcgt gcgcccttgc ggaaggactc gaacgcgtgg cgaccggcgc
    27121 cacagcccat atcgaggacg accatgcccg gtcgcaggtc gaacaattcg tagtcgacgg
    27181 tcaacatcgg tgggggttct ctcagctctc ggccagaagg gcacgatagt gctcgacggt
    27241 acgttcggcg gtgttgcgcc agctccagcg ggtgatnnnn nnnnnnnnnn nnnnnnnnnn
    27301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27361 nnnnnnnnnn nnnnnngcgc acgccgactc gttgtacagc tcgacgatgc gctcgtcgga
    27421 cacgccggac acgaactcga cggcgtccca gagcccgagc tcctcgatcg tgcgctgggc
    27481 gatggagccc tccttgagcc ggccgatcag cacgagcttg aggtcgggat actcggtccg
    27541 gagcttggcg agcgcctcga gcaggaaccg ttggcccttc atcgcgacgt cggccgacgc
    27601 agtggagatg atctggtggg gccgacgctc gacgccgggc agcggcttga agaggtcggg
    27661 atccacgccc acgggcacca cgaacagctt ctcgggcgac acgtcgtggg tccggcagat
    27721 gtcgtcgtag ctgttctggg acacggtgat gatgcgacgc aggcgcttgg cgactcgggt
    27781 ctgcatcttg gtgaaggcgt accagcgggc tttcgcgtac ttctggtagg tcgtctcggc
    27841 gtgctccatc tcgatgcgac ggtcgaccgt gatcggatgg tggatcgtgc cgaggacggg
    27901 gaggccggac ttctcgatgg cgagcaggcc gtagccgagg cactggttgt cctgcacgag
    27961 gtcgaactcg tgggtgcgac gcttgaggtg gtcccaggcc cgcagcgaga acgccagggg
    28021 ctcggggaac gttcccgtgc tgaacgccgt gacctcggcg aagtccatcc aggtcttcag
    28081 ctcccacagg ccgggcatgc gcatcgggaa gtggtcgttg tagatgtcga ggctgggcag
    28141 ttcgacgagc gggacgcgct cgtcgacgat cgggtacggc tggcccgaca gcacctcgac
    28201 gtggtgcccg aggtcgacca gtgccttgct gaggtgtcgg gtgtagacgc cctgcccacc
    28261 cacgtggggc ttgccccggt aggtcaggta ggcgatccgc agcggatcgt cgggaccggg
    28321 gcgtcgtctg ctgttcggtc cggcgtcagt accagtttgc ataaggttcc agcttccttg
    28381 gccgcttggt gatcggtcaa aaccgggcaa ccggttttcg gggggtttga ttcggtccaa
    28441 gggcaggtgg agtctcccac gcccatgagc gctctcaggt tccggtcgga tcgggggaga
    28501 acgacaacgg ggcgacgccc accagcgtgg acgtcgcccc gtcgcgacgt gctcggatgt
    28561 gacggcccga ggccgtcggg cctgaccgtg tgggtcagcc gttgtcgccc atgcggtacg
    28621 gcgtcgatgg tgaccgtgtg ggtcagccgt tgtcgccgac ggtcaggtcg atggtgtagc
    28681 tcttgaagcc gccgtcgaa
//
LOCUS       DAOSOA010000010        27896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_1712_length_27896_cov_8.322474, whole genome shotgun sequence.
ACCESSION   DAOSOA010000010 DAOSOA010000000
VERSION     DAOSOA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 27896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27896
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1712_length_27896_cov_8.322474"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKY13_01635"
     CDS             <1..345
                     /locus_tag="PKY13_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT78652.1"
                     /translation="AGTVLPRDTAVNMVISSGPPPVALPKVTGLKRADAAAELSKLGL
                     IIGEVTQTGAVADPLDGVVLSQSPDPGTELRAGDVVTVTVRRAAVPPPTLPPTTVPPA
                     APATPAAPGPGQ"
     gene            402..1370
                     /locus_tag="PKY13_01640"
     CDS             402..1370
                     /locus_tag="PKY13_01640"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT78653.1"
                     /translation="MNGPLVVTLICLIAGLGLSLWAFTDSLEGSRGIQLPRIEVPDVK
                     SMALDQAKGELERAGFVVEVQFQPNEDPLKPKGVIIGQKPLSGSKAEQGELVVIRVSD
                     GPLGQSVPGVEGQQAIDAVATLQANGLIVEQIPTPSETVRANEVIATDPPSGARVPAA
                     GAIKILVSSGPAPRLVPPILDKPLEQALADIGRSGLAVGKIKRVFRADLVPGTVFETD
                     PAVGSPLPRDTPVGLSVAGPEPTTKVPYLVGLRQASAERVLKSAGLKVKIATSPVAAG
                     DASEGKVISQGTPPQVEVKEESVVEITVAVVVVPVPAPTTAAPPGG"
     gene            1395..2051
                     /locus_tag="PKY13_01645"
     CDS             1395..2051
                     /locus_tag="PKY13_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78654.1"
                     /translation="MEAASLDGHVGGFTIEESARRLGHYRWASIRLFEVLGRWVASVP
                     EPNVKVFLAAASRHHGWHADLLRERLPEVRELHPDRQTVPADARLVELFDELESLRGP
                     DVTAERLTGVYGVVIPHLIEVQSWHLDRCTDVADASVMRTLGFVLRDEVGDVEAGRRL
                     LEQLGSGMDSAARAAAFRSTIEELLSTAGGISGPGTLVDTREISGRSSSAPDDVDVPG
                     "
     gene            2220..3158
                     /locus_tag="PKY13_01650"
     CDS             2220..3158
                     /locus_tag="PKY13_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT78655.1"
                     /translation="MAKDRVDRDEEDLVRLYLTDIGQYPLLTKDDEVRLAQAIESGVA
                     AATEFEQLGRTASAARKRELRRQIRAGEEAERTFVQSNLRLVVSIAKKYQASGLPLLD
                     LIQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDTLA
                     RLQKARARLELKLGRPATLSELSAEVEMPEDKVTEALRFAAEPLSLSEPLREDGDAEL
                     GDVVEDRGAESPFEVAATALLPEEISRLLSPLDEREREILKLRFGLDRGEPRTLEEVG
                     EHFHLTRERIRQIEARAMSKLRHPSSDTGARDLLAV"
     gene            3274..3939
                     /locus_tag="PKY13_01655"
     CDS             3274..3939
                     /locus_tag="PKY13_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78656.1"
                     /translation="MLYPLWTAKPGSGCSTISLALAARLAAKRDLDVLVVDLDGDLTA
                     AAGVAPRHDGVTDWLAAGSVGSDALRRLEVELRPRLSLLPVGSSHAWPDDRADTLVRA
                     LHADPRLVVVDVSTVTPHALSSMQRLRHRLAADDPSLLVTRSCYLAVRRAQALPIRPT
                     GVVLVREGGRTFDRHLIAEALGAPVVAQIDHDPAVARAVDDGKLVRRPPRPLSRQVEA
                     LVR"
     gene            3936..5042
                     /locus_tag="PKY13_01660"
     CDS             3936..5042
                     /locus_tag="PKY13_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT78657.1"
                     /translation="MTSPTDQVIQRRFLDDPRRDVPARMLLAEIIADELPLADAAESA
                     AILERLHASLLGLGRLDPLLHDTTVTDVLVNGDGRVWVDRGGHLVPGGLVMDVDEIRL
                     VVERAFRRNGLAVDRSHPIGDTRLADGSRISVVLPPLAPDGIHVAIRTFSVERVGLDA
                     FGDANVAERLRRDVGRHANIVVFGPTGSGKTTLVNALATGIDPSHRVVTIEDAAELRL
                     DLPHVVRLEGRPDNGDGAGRVDLRTLVRAALRLRPDRIIVGEVRGAEALDMVWAMATG
                     HDGSLSTCHAKSAADALARLETFVLLADGELPHSAVRSQVRSAVDVVVGVRRIGPQRR
                     VVSVHDVVTDPGHPTGVEPVYADGRWFDRAGSRA"
     gene            5039..5743
                     /locus_tag="PKY13_01665"
     CDS             5039..5743
                     /locus_tag="PKY13_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT78658.1"
                     /translation="MSAPSWGVALAVLVPMARQVFARRRDRAGLAPVETARAGFGRRR
                     RRSSRRRARREGAQVVAELPRLAEAVARDVRAGVTVTDALVGARTLVGGPLAADLTQL
                     DRALGAGASLADALVAWRDDRSAPALDQFVAAVLLGAELGSRAAPAFEAVAAALRDQA
                     EVADEVRALTTQSRASATLLTGLPPLACTGLAVIEPATARFLFRSSAGLACVGTAGAL
                     VAAGWWWMDRLCRAER"
     gene            5740..6516
                     /locus_tag="PKY13_01670"
     CDS             5740..6516
                     /locus_tag="PKY13_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT78659.1"
                     /translation="MSPVVVVMWCVVAGVGAWWFRPRTSRTVGPLHPGGADAPVGGPS
                     LLDRVGRSVTAVVPALEPVPARHVSLILLAGVVSAPLVGAWSAALVLVALACARGAGR
                     RRETRQLDRDLTAVATIADLLGIALGSGLALGPAIVAVQQRLRPGDDAGLAGVVVDLE
                     RGRSVDEALDAWAGRSQPVGELAALLRGAGRSGAAIVDALARLGNDVRRRRRRRAEER
                     ARRLPVAMLLPLVTCVLPAFGLVTVVPMVAVGLGRLGPPV"
     gene            6611..6808
                     /locus_tag="PKY13_01675"
     CDS             6611..6808
                     /locus_tag="PKY13_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78660.1"
                     /translation="MFRFPFLHRLAALERGQSTAEYALVLVAVTSMVAVVIAYVSGDG
                     GGLITGLFGAAFAKMRSLVGG"
     gene            6808..7194
                     /locus_tag="PKY13_01680"
     CDS             6808..7194
                     /locus_tag="PKY13_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOT78661.1"
                     /translation="MPIATLPPRSDRAQATVELALVMPVVAVLVLVVLQVVVVMRDSV
                     ALTAAARAAARRAMVDPGPASVRAAAVGETRLSPARLSVSIGGSTEPGGYATVTVRYS
                     SPTDVPLVGAFVGDVALSERFVVLRE"
     gene            7248..7577
                     /locus_tag="PKY13_01685"
     CDS             7248..7577
                     /locus_tag="PKY13_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT78662.1"
                     /translation="MYFHVRRGEQGRWTMVHVLGEIDLATAPQYRSELIAAASASTAL
                     AIDLTDCDLIDSVGLGVTVGGARRMWGAGGQFAVVTNDRIRRTFERCRLDEILDLVDA
                     PEALPAA"
     gene            7574..8005
                     /locus_tag="PKY13_01690"
     CDS             7574..8005
                     /locus_tag="PKY13_01690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT78663.1"
                     /translation="MIPTHTTELDIPAESRFVATARNFVADAARHGGWMDDQRLDDLR
                     LVVSETVTNALHAQATSSVDELIHVVAAVYADRIEIEVTDSAGGFEPPDEAPALPEPD
                     LRREGGFGLPLIEALSDEASFTRTPSGTRVRVVVFRDPPAA"
     gene            complement(8034..10403)
                     /locus_tag="PKY13_01695"
     CDS             complement(8034..10403)
                     /locus_tag="PKY13_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT78664.1"
                     /translation="MTRDGLTAAEVEDRVRRGLVNEVPPAPSRTVTEILRANVLTRFN
                     ALIGLLVVIVLASGSPQDALFGGVIIANSVIGIIQEVRAKRTLDQLAVLSAPAAHVVR
                     DGKVVEMSVDHIVLDDVIELRPGGQVVADAEVIESDALEIDESLLTGEADPEVKSPGS
                     ELFSGSFVVTGSGRARVTKVGADAYAAKLAEEARRFSLANSELRTSIDKIVTWVSWAL
                     IPAAIGLVWSQLRQPGKSVGDALVAAVAGMVGMVPEGLVLLTSVAFAVGVVRLAKRRT
                     LVQELPAVEILARVDVVCLDKTGTITEGTMVVDRLECVSDEYDATSIDNALAAIAWAD
                     PNPNPTQQALADRYAEAPLDWTRVGGVPFSSARKWSATGFEHHGWWLFGAPEMILAES
                     AFAAVADRVSAAAEDGLRVLLLAHSSEAIDADADGLPDDLGAVALVMLTDKIRPDASA
                     TLEYFAQQGVTLKVISGDNPATVAAVAARAGLAGAESAVDARTLPDDPEALAEAVEAT
                     TVFGRVSPHQKREMVKALQSRGHTVAMTGDGVNDVLALKDADCGIAMASGSEATRAVA
                     QIVLLDSSFAGLPAVVAEGRRVINNLQRVAGLYLTKTTYAVIFAFVTGALALDFPFLP
                     RHLTLISTLCIGVPSFLLALAPNTDRVRGDFLRRVARDSVPFGVICAVATLVTYVVAR
                     RHYTPADPQSLALMEQQTAATMTLAGCSLIVLLRNARPLVGWKLALVIAMGGAVALAF
                     VTPMGRDFFALNLPPTPILITALLAVAAAGAAMMVAHRVFHEPRTRPGL"
     gene            complement(10456..11664)
                     /locus_tag="PKY13_01700"
     CDS             complement(10456..11664)
                     /locus_tag="PKY13_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT78665.1"
                     /translation="MDFQLSPTALALQDKLLAFMDECVYPAEAVYEEQMAALGDPHAH
                     PQIVEDLKAEARTRGLWNLFLPHETQWTEGLSNLDYAPLAEITGRSIHLAPEALNCAA
                     PDTGNMEVLTMFGTDAQKEQWLQPLLEGEIRSAFAMTEPDVASSDATNIQLSIVRDGD
                     EYVLNGRKWWISGAASDRCKIFIVMGKTDPTASRHLQQSMILVPRDTPGLTVVRDLPV
                     FGYQDREGHVELRFEDVRVPVENIIAEEGAGFMIAQARLGPGRIHHCMRCIGIAERAL
                     ELMCRRVTDRVAFGRPLAEQGVIREWIADSRMEIEQARLLTFKAAWLMDTVGNKGAAV
                     EISSIKVVVPNMALRVVDRSIQAHGGGGVSDDFPLARMYAGLRTLRFADGPDEVHRRQ
                     VARTELGRYS"
     gene            11850..14651
                     /gene="topA"
                     /locus_tag="PKY13_01705"
     CDS             11850..14651
                     /gene="topA"
                     /locus_tag="PKY13_01705"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT78666.1"
                     /translation="MGNPLVIVESPAKARTIGRFLGDDFVVESSVGHIRDLPSKAAEI
                     PPSYKGVKWASLGVDTENHFKPLYVVSPNKKDQVRHLKELLKDADELYLATDEDREGE
                     AIAWHLLEVLNPRVPVKRMVFHEITPDAIQHAIDNPRELDRRLVDAQEARRIFDRLYG
                     YEVSPVLWKKVMPRLSAGRVQSVATRIVVERERERMRFVTASYWDLDATFQLTGNDPT
                     GAPRDQTPFAGSLLEVDGARVATGKDFDESGRLTKADALLLSETDAAQLRTALTDQPV
                     RVRSVERKPYTRKPYAPFMTSTLQQEAGRKLRFSSSQCMSLAQRLYENGFITYMRTDS
                     TTLSETAINAARSQISEKYGAAYLPDAPRLYSKKVKNAQEAHEAIRPAGDTFRSPDEV
                     RSELGSQEFRLYELIWQRTIASQMTDARGESVAVRLEVDATDGRQALFASSGKTITFP
                     GFLRAYVEGADDPDAELEDQERILPEMAEGDAARSLELAAKGHETQPPPRYTEASLVK
                     RMEEIGVGRPSTYASTISTIQDRGYVRKKGSALVPSFVAFAVVTLLERHFPDLVDYAL
                     TAHMEDDLDQIAGGKADAEPWLSRFYFGTTGDGDPASARPGLKALTSDIEGIDARDVN
                     SIVLGADANGVPIVARVGRYGAYLQHEDETANIPDDISPDELTVEKCLELLATPKERQ
                     LGIHPTENLPIFVKNGRFGPYVQLGQHDDETGFKPKMASLFKTMTVERITLEDALELL
                     SLPRVVGVDPADGVEITAQNGKFGPYIKKDTDSRSIEDEEQLLTLTLDEALRILAEPK
                     RRRGQAVKGPLKEVGPDPDSGKPIVVKDGRFGPYVTDGETNASLRKGDIVEELTMERA
                     LELLADRRAKAPAPKKAAAKKAPAKRAPAKKKAAAKKAPAKKKAAAKKAPAKKSATAA
                     VTPKAVADAFIASAGDEPF"
     gene            14675..16123
                     /locus_tag="PKY13_01710"
     CDS             14675..16123
                     /locus_tag="PKY13_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT78667.1"
                     /translation="MTATLGEPLRVRWFGSIEFFWLWISQLISAFGDWVGLFAVTALA
                     ANISNRPEAATALVLTARVAPSFFLGPFMGVLVDRYDRKLLMRIADLARALVFCALPF
                     ATTLWHLIVASLLLEVFTLLFAPAKEALVPVLVPRDRLTSANSLGILAAYGTMPLAGV
                     AQTLLKAGNDRLAHVEWLGPLGFGRDIGSTQTLAFYFNALTYVAAFVIVRTLIRTKAE
                     PVHERVGTVEESDAPSGIRAVLAEVREGWKEVVVNPIVRAVNVGLAAGLLGGAMLVPL
                     GPTFAKNIVGDVDKFPLYITALGCGVALGVGLLTAFQKKLPKEKVFCGALFFAGGSIL
                     FGISMSTFWLSAVGVFGLGLGAGAVYVLGYTLLQENTVDDMRGRTFNTFLTLVRLAVL
                     VAMVAGPTISSVLDPLMKRWIGGRTALGTPGVRWFGVEYGLPGVRVTLWFGGLLILVA
                     ATLASRSIDPSAVRVGRARRRDTVAAGSEELT"
     gene            16120..16725
                     /gene="tmk"
                     /locus_tag="PKY13_01715"
     CDS             16120..16725
                     /gene="tmk"
                     /locus_tag="PKY13_01715"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT78668.1"
                     /translation="MTGRFIVFEGGEGSGKSTQAAILADRLDAVLTRQPGGTDIGAAL
                     RAILLDTGTVALAPRAEALLMAADRAQHAAELIRPALAAGRHVVCDRYTYSSVAYQGH
                     GRGLDPQRVRDISAWATEELWPDLVVLLDVDPDAAATRLVRELDRFEQAGDEFHRAVR
                     DGFLAQALAEPERWVVIDGTGTVDGVARAVWAAVEPVMESE"
     gene            16736..17797
                     /locus_tag="PKY13_01720"
     CDS             16736..17797
                     /locus_tag="PKY13_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78669.1"
                     /translation="MTETALETELDLWEAVVGQDEIVAQLRSAAADPTHAYLFVGPPG
                     VGTMAAARAFAAEILSRGATPEQAERHRRLAAADHHPAVIVVERVGASILAAQAREIV
                     RQAQMRPPEGDLQVLILTEFHLVTVAAPMLLKSIEEPPAGTIFIVVADEVTPDLVTIA
                     SRCVTFEFPHLSRKVIEERLRLEGAPADVAEAAAVGSGGDLGRARLLATDEQVVDRRN
                     FWFSLPERLDGTGSAAARLAAEVIRRVEEVLAPLAAMQAAEMEELVEQAEKLGQRKTA
                     LKEVEARHNREKRRIRMDELRAGLAALMDGYRTVAATEPQTYLDAARLVGELTDHLQF
                     NPNEELALQALFVSLPRRR"
     gene            complement(17808..18923)
                     /locus_tag="PKY13_01725"
     CDS             complement(17808..18923)
                     /locus_tag="PKY13_01725"
                     /inference="COORDINATES: protein motif:HMM:NF027219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HOT78670.1"
                     /translation="MLAFTCDNCGQLVFFDNTACLACLAPLGVIPGSGVITLQPDDHD
                     PSRYRRFTDPEGIWWRRCVNASTTMCNWLVPDTEPGDRCRSCELTRTLPDASDVEAQH
                     ALTVAEAAKRRLLFQLDSLGMPVVSRSVDPAHGLAFDLLSSRYTHVTTGHADGVITID
                     LAESDDAHREWLRKTMGESYRTMLGHLRHEIGHYYWMLFTQDDPDVLARIREQFGDDT
                     ADYQAALDRHYAGGPPPGWEEHYVSNYATTHPWEDWAETFAHYLHIRGTLQTAAAYGV
                     YIEGPAALRNSPTSNRFRSDPAHAVANASFEVLMKDWVPLTYALNEINRAMGKDRLYP
                     FVLRPNVVEKLSLVHDLCVAAATATKAAEAATLQSSV"
     gene            19023..19664
                     /locus_tag="PKY13_01730"
     CDS             19023..19664
                     /locus_tag="PKY13_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT78671.1"
                     /translation="MNRRVLIVDPDPLTRELHRMALTFVGHDVRVVASSNDAELLFDG
                     FGPDVVVLTVDIGPEDATRFRDRLVRSGDPVRQVVIVGDQADRRRPGAAVVLARPLDL
                     DALVAAVEGGTGGESTVSVGTLELDRRRERVVVAGQEVHLSPTEFRLLRLLAINAERV
                     LSKADILDQVWRYDFGGRTNAVETYVSYLRRKLGPLGGPSIVTVRGAGYVLRA"
     gene            complement(19673..20308)
                     /locus_tag="PKY13_01735"
     CDS             complement(19673..20308)
                     /locus_tag="PKY13_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78672.1"
                     /translation="MISSITSGASALDLATLQRLSSTATSSTNTDETARSGRTGGHRP
                     PGPPPEAIESAAKALDMSESDVRDALAGGSTLEDLATSKGVSKDDLLAAVTDGLAAGA
                     AKHGRQLSADQITEFATHLIDGTQPPPPTGGAAGGADSDLTASGLRGVDTGAGHTRGH
                     HGPPPGTSIEDDNTSTTGASATAGAQRAYQAFTAEQLLASLSTGQLADLAA"
     gene            20445..21704
                     /locus_tag="PKY13_01740"
     CDS             20445..21704
                     /locus_tag="PKY13_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78673.1"
                     /translation="MRVRFIPVLAASLLLTACGGGDDGAITIGSSKAPGGAATFDADQ
                     CAVFESEDGPDEKFVESMPDRYRDAATAIAEFGTSMDAMGDNDDGSIDGLVASLTKDG
                     VAEQLREFAADIEEQCGSSEGSAVIGGIATASEMASAPKDADYCDGIEAGFSTDDGGA
                     EEIAALADIAPESHREPLAALAAVDPESMDSADQDALFGPLFGLGIYAEDQCGIEGAL
                     AQMLLGAMFLGAGDGMTTTTLAEGVDPAQYPDITADAANAALPAGSGIAFQVVSADLD
                     DDSEYLASMVVPEGWTSDTGFNVEFSPPADSGISIFTSIEAGAGCDGTCEATDWDERL
                     RGESGFLTQFFSSHPGAAEAPIAGSEGVVVTNSTEDEAVALVLRWDDGADKYFSCEVS
                     LEPEQADLLPAFVAACESSRPAWFAVA"
     gene            complement(21713..22912)
                     /locus_tag="PKY13_01745"
     CDS             complement(21713..22912)
                     /locus_tag="PKY13_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT78674.1"
                     /translation="MVKDPASTDWHTRFDHYDPAYAADPFTVWSALRTDCPIARSERF
                     RGMWVPTRHADIEAIARDTTHFSSRSPLVAQFGSMSDFGFVVPPISSDPPYHTAFRQL
                     LLPFFSPRRIEAMGPIVTDLCDALIDDFAGDGGTISFDAAQQYAQHIPVHVIARMLGV
                     PDADGELFRKWVHEMLELAPTDLDVAISALTEFYLYFEAQIAERRTAPGDDLISFLIS
                     AELDGRPLTDNEVFGGCLLLLIAGIDTTWSAIGSTIWHLAQHPEEQRQLRDDPALWSD
                     ALEEFLRAYAPVTMAREVVEDVTLGGCPMHQGDPVLLPFPAANRDPEAFDDPDAIRLD
                     RQPNRHLAFGVGIHRCLGSHLARLEVRIAGERLLARVPEFRLADPSAVTWSVGQVRGP
                     RRLALLA"
     gene            22962..23573
                     /locus_tag="PKY13_01750"
     CDS             22962..23573
                     /locus_tag="PKY13_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT78675.1"
                     /translation="MAATTERANDRANVGARSDRSAELVAAAIAVVAGGSLDALDAAS
                     VAREAGVSKALVYYYFPTHLDLQAAVLRSAAEDLLEAIAHAVTAAAPAGTPITAGLDA
                     AVAYIERHPAAYVALARTAGYHPKLSEVFEFARDGVVDLMATAFGIDEPTQRQRIALR
                     SWIALAEEAVLHWIVADRPIERSEIVDYCRAVAEFVATTPLAR"
     gene            23578..24768
                     /locus_tag="PKY13_01755"
     CDS             23578..24768
                     /locus_tag="PKY13_01755"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOT78676.1"
                     /translation="MSGPLHDLRVIEMGQLLAGPFCGQLLADFGADVIKIEPPGQGDP
                     MREWGREKAGGTSLWWPVVARGKRSVTLNLREAEGQEIVRRLVADADILIENFRPGTL
                     ERWGLAPSDLMALNPRLIVVRVSGYGQTGPYADRPGYGAIGEAMGGLRGVIGEPDRPP
                     ARAGISIGDTLAAIFACVGTLVALHSRSNTGAGQIVDSALYEAVLGVMESLIPEYTIA
                     GYVRPRTGSILPNVAPANAYPTADGEHLISGNQDTVWRRLAVAMGRPELGDDERFATH
                     NARGEHQSELDDLLGDWTRTLTSAELEAVLNEHSVPNGKIYTAPDMLADAHFAAREAI
                     VTMVHPLLGDFPMQNVVPKLSDTPGEVRWVGPELGEHTDEVLTELLGLDDETRAGLRE
                     RGVI"
     gene            complement(24799..25641)
                     /locus_tag="PKY13_01760"
     CDS             complement(24799..25641)
                     /locus_tag="PKY13_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT78677.1"
                     /translation="MTDTLAPDLAPADVDSILSALASGERPKRPNALSVSITHGWRAL
                     LKIKHVPEQLFDVTIFPIMMTLMFTYLFGGALAGSTKAYLQFFLPGILAQSVLFITMY
                     TGVGLNVDHEKGIFDRFRSLPMWRPSAIVGPLLGDAVRYSIASTVIIVMGLILGFRPD
                     GGVVGVVASVLLLLVFAFSLSWIWTMFGLILRTEKSVMGMSMMLLFPLTFASNIFVSP
                     DTMPGWLKAFVDVNPISWLVTSVRGLMHGEASGSDIGKTLIACAVLVAVFAPLTMRAY
                     RNKA"
     gene            complement(25638..26651)
                     /locus_tag="PKY13_01765"
     CDS             complement(25638..26651)
                     /locus_tag="PKY13_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT78678.1"
                     /translation="MTSTLTAPHAIEARGLVKVFGDTRAVDDLDLTIPTGGVYGVLGP
                     NGAGKTTVIRMLATLLRPDGGGATVFGHDIVDDADTVRSMVSLTGQFASVDEDLTGME
                     NLRLLAKLLGHRGAAATSRATELLTAFGLIDAADKQAKHYSGGMRRRLDIAASIVVTP
                     DLLFLDEPTTGLDPRSRNQVWDIVRALVAAGTTVLLTTQYLDEADQLAGRIAVIDHGK
                     VIAEGTPGELKASVGAGALHVRVADPERRPEAQSILQRHLDRPVALEPDPAALSARVD
                     DAERVAAGLSALSQAAIDIATYAFGQPTLDEVFLALTGHTAESSSDPDHPLDSQHKEE
                     ARS"
     gene            26799..27773
                     /locus_tag="PKY13_01770"
     CDS             26799..27773
                     /locus_tag="PKY13_01770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT78679.1"
                     /translation="MQWRTNTLRERRKRRVALVALSACALLAASCGDDPGNISIGPRA
                     TTSVAETTTTTVAETTTAPPESTTAVPETTTAPPQSIVPATVAPALPGDHQGEGDTAA
                     GPRPEVVRPPAAWIGFGQPQPGSMFENLHEDHALDGKGKFVAFTFDDGPSQYTKPIFD
                     ILKFMGVQATFFQISRQSLANQDQVKEMLAAGMHIGSHTINHPHLAEIPPDQQQAEVL
                     GSIDQMNAAYGAGTVKCFRPPYAQYDQHVLDLVNQRGAATAMWSLDTLDWKKPAWQSL
                     VTRVVDGAQDRNVLLFHDGGGDRTQTILALPWIIQGLRDKGFQFIPVC"
     gene            27822..27894
                     /locus_tag="PKY13_01775"
     tRNA            27822..27894
                     /locus_tag="PKY13_01775"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27855..27857,aa:Thr,seq:cgt)
BASE COUNT         4180 a         9545 c         9793 g         4378 t
ORIGIN      
        1 gcgggcacgg tgctgccccg cgacaccgcg gtcaacatgg tgatctcgag cggaccgcca
       61 ccggtcgccc taccgaaggt caccggcctc aagcgggccg acgccgccgc ggagctgtcg
      121 aagctcggcc tcatcatcgg cgaggtgacc cagacgggag cggtcgccga cccgctcgac
      181 ggcgttgtgc tgtcccagag cccggacccg ggaacggagt tgcgcgcggg tgacgtcgtc
      241 acggtgacgg ttcgtcgcgc tgccgtcccg cccccgaccc tgccgcccac gacggtgcca
      301 ccggcggcac cagcgacgcc tgccgcgccg ggaccgggac aatgaccgcg gccgttcgac
      361 actcggccga aggcgccccc gaggtcatcg atccccggcc catgaacggc ccgctcgtcg
      421 tgacgctcat ctgcctgatc gcgggtctcg ggctgtcgtt gtgggcgttc accgacagtc
      481 tcgaggggag ccggggtatt cagctgcccc ggatcgaagt gcccgacgtg aagtcgatgg
      541 ccctcgacca ggccaagggc gagctcgagc gagccggttt cgtggtcgag gtgcagttcc
      601 aaccgaacga ggatccgctc aaacccaagg gcgtgatcat cggtcagaag ccgctctccg
      661 gttccaaggc ggagcagggc gagctcgtgg tcatccgggt cagcgacggc ccgctcggcc
      721 aatcggtgcc cggcgtggaa ggccagcagg ccatcgacgc ggtcgcgacg ctgcaggcca
      781 acggcctgat cgtcgagcag atcccgacgc cgagcgaaac cgtccgggcc aacgaggtga
      841 tcgccaccga cccgccgtcc ggcgcccgcg taccggcagc cggcgcgatc aagatcctgg
      901 tctcgtcggg ccccgcaccg cgactcgtcc caccgatcct cgacaagccg ctggagcagg
      961 ccctcgccga catcgggcgg agcggcctcg ccgttggcaa gatcaagcgg gtgttccgag
     1021 ccgacctcgt gccgggaacc gtgttcgaga ccgacccggc cgtcggctca ccgttgccgc
     1081 gcgacacacc ggtcggcctg agcgtcgccg gccccgaacc cacgaccaag gtgccgtacc
     1141 tcgtcggcct ccgccaggca tcggcggagc gcgtgctcaa gagcgccggg ctcaaggtca
     1201 agatcgccac ctcgcccgtc gccgctggag atgcctcgga gggcaaggtc atctcacagg
     1261 gcacccctcc ccaggtcgag gtcaaggaag agtcggtcgt ggagatcacc gttgccgtcg
     1321 tcgtggtgcc ggtccctgcg cccaccaccg cggcgccgcc cgggggctga cgccctcgcc
     1381 ggtagattgg ccgggtggaa gcagcatcgt tggacgggca cgtcggtggg ttcacgatcg
     1441 aggagtcggc tcgtcgcctc ggtcactacc ggtgggcgtc gatccggctg ttcgaggtgc
     1501 tcggcaggtg ggtcgcctcg gtccccgagc cgaacgtcaa ggtcttcctc gccgctgcct
     1561 cccggcacca cggttggcac gccgacctgc tccgtgagcg acttcccgag gtccgcgaac
     1621 tgcatcccga tcgtcagacg gtccccgcgg acgcccgcct cgtcgagctg ttcgacgagc
     1681 tcgagtcgtt gcgaggtccc gacgtcaccg ccgagcgcct caccggcgtg tacggcgtgg
     1741 tgatcccgca cctcatcgag gtccagtcct ggcacctcga tcgttgcacc gacgtcgccg
     1801 atgcgtcggt catgcgcacc ctcgggttcg tgcttcgcga cgaggtcggc gatgtcgaag
     1861 ccgggcgacg tctcctcgag cagctcggca gcggcatgga ctccgccgca cgagctgccg
     1921 cgtttcgctc caccatcgag gaactcctct ccactgcggg cggaatatcg ggccccggaa
     1981 cgttggttga cacccgtgag atcagcgggc gctcaagttc tgcacccgat gatgtcgatg
     2041 tcccaggctg acgcagccgg aaccggttgc gcgtgccgtc ccaccccgcc ctccgacgcc
     2101 cacaaccccc atcccaccta cgtttcgggc agaaagggtc ttgacggact ccgtatcgtt
     2161 agagagcgtt atccatcttc ccccgctgga gatggctgac tggaagcgaa ggttgcaacg
     2221 tggcgaaaga tcgtgtggac cgcgacgaag aagacctcgt ccgcctgtat ctcaccgaca
     2281 tcggtcaata cccgctcctg accaaggacg acgaggtccg cctcgcccag gccatcgagt
     2341 ccggcgtcgc cgcggcgacc gaattcgaac agctcggtcg caccgcgagc gcagcccgca
     2401 agcgtgagct ccgtcggcag atccgtgccg gcgaggaagc cgagcgcacc ttcgtccagt
     2461 ccaacctccg tctcgtcgtg tcgatcgcga agaagtacca ggcctccggc cttcccctcc
     2521 tcgacctgat ccaggaaggc aacctcggtc tcatgcacgc cgtcgagaag ttcgactggc
     2581 gtaagggctt caagttctcg acctacgcca cgtggtggat tcgccaggcg atcacccgtg
     2641 gcatcgcgaa cacgggccgc accatccggc tccccgtgca cgccggcgac accctggcgc
     2701 gcctccagaa ggcccgggca cgactcgagc tcaaactcgg ccgtcccgcc acgctctcgg
     2761 agctgtccgc cgaggtcgag atgcccgagg acaaggtcac cgaggcactc cgtttcgcgg
     2821 ccgaaccgtt gtccctctcc gagccgctcc gcgaggacgg cgatgccgag ctcggcgacg
     2881 tggtcgagga tcgtggcgcc gagtcgccgt tcgaggtcgc cgccaccgcg ctcctgcccg
     2941 aggagatctc ccggctgctc tcgccgctcg acgaacgtga gcgcgagatc ctcaagctcc
     3001 gcttcggcct cgaccgtggt gagccgcgaa ccctcgaaga ggtgggtgag cacttccacc
     3061 tcacccgtga gcggatccgc cagatcgagg cgcgcgccat gtcgaagctg cgccacccca
     3121 gctccgacac cggcgcccgc gacctgcttg ccgtctgacg ttcgatcggg cgggtcgaac
     3181 gacccgttga ccaccggcgg ccggcacgcc tagcttcggc ggttcttccc ctctgtagtc
     3241 ggcgcctcgt gcgcatactc ctacggagcc atcgtgctgt accccctgtg gacggccaag
     3301 cccgggtcgg gatgctcgac catttccctc gctctcgcgg cgcgcctcgc cgcgaaacgc
     3361 gacctcgatg tgctcgtcgt cgacctcgac ggcgacctga cggccgccgc aggtgtcgcc
     3421 ccgcgtcacg acggtgtcac cgactggctg gccgccggat cggtcggttc ggacgcgctc
     3481 cgccgcctcg aggtcgagct ccgaccgcgg ttgtcgctct tgccggtcgg ttcgtcccac
     3541 gcgtggcccg atgaccgcgc cgacacgctc gtccgggcgt tgcacgccga tccgcgactg
     3601 gtggtggtcg acgtctcgac ggtgacgccg cacgccctgt cgagcatgca gcggctacgt
     3661 caccgactcg ccgccgacga tccgtcgctg ctcgtgacac ggtcctgcta cctcgccgtc
     3721 cggcgggcgc aggccctgcc gattcgcccc accggcgttg tcctcgtgcg ggagggcgga
     3781 cggaccttcg accgtcacct catcgccgaa gcgctcggcg ctcccgtcgt cgcccagatc
     3841 gatcacgacc ctgccgtggc acgagccgtc gatgacggca agctggttcg acggccaccg
     3901 cgcccgctgt cccggcaggt cgaggcgctg gtccggtgac gtcccccacc gatcaggtca
     3961 tccaacgccg gttcctcgac gatccacgcc gcgacgtccc ggcacggatg ttgctcgccg
     4021 agatcatcgc cgacgagctg ccactggccg acgccgccga gtcggcggcc atcctcgaac
     4081 ggttgcacgc gtccctgctc gggctcggcc ggctcgaccc gttgttgcac gacacgacgg
     4141 tgaccgacgt gctcgtcaac ggtgacggtc gggtgtgggt ggatcggggc ggccacctcg
     4201 ttcccggtgg actggtgatg gacgtcgacg agatccggct cgtcgtcgag cgggcgttcc
     4261 gtcgcaacgg cctcgccgtc gaccggtcgc atcccatcgg cgacacccgt ctcgccgatg
     4321 gatcgcgcat cagtgtggtg ctcccgccgc tcgctcccga cgggatccac gtcgcgatcc
     4381 ggacgttctc ggtcgagcgc gtgggtctcg acgcgttcgg ggatgcgaac gtcgccgaac
     4441 ggctgcggcg cgacgtcggt cgccacgcca acatcgtggt gttcgggccg accgggtccg
     4501 ggaagacgac gctcgtcaac gccctcgcta ccgggatcga tccgtcacac cgggtcgtga
     4561 cgatcgagga tgccgccgag ctccgcctcg acctgccgca tgtcgtgcgg ctcgaaggcc
     4621 gccccgacaa cggcgatggc gccggacgag tcgacctccg aacgctggtt cgcgccgcgc
     4681 tccggctgcg ccccgaccgg atcatcgtgg gcgaggtgcg cggtgccgag gcgctcgaca
     4741 tggtgtgggc gatggcgacg gggcacgacg ggtcgctgtc gacctgtcat gcgaagtcgg
     4801 cggccgatgc cctcgctcga ctcgagacct tcgtgctgct cgccgacggc gagctgccac
     4861 actcggcggt ccgctcccag gttcgctcgg cggtcgacgt cgtggtcggg gtgcgacgga
     4921 tcggcccaca gcgccgcgtc gtgtccgtgc acgacgtggt gaccgatccg ggccacccca
     4981 ccggtgtcga accggtctac gccgatggtc gctggttcga ccgcgccggg agtcgggcat
     5041 gagcgcgccg tcctggggtg tcgcgctcgc tgtcctggtc ccgatggcgc gacaggtgtt
     5101 cgcccgccgt cgcgaccggg ccggcctggc ccccgtcgag accgcgcgtg ccggtttcgg
     5161 tcgtcggcga cgccgctcgt cccggcgccg tgcccgccgg gagggggcgc aagtcgtcgc
     5221 cgagctgccc cggctcgccg aggccgtcgc ccgcgacgtt cgtgccggcg tgaccgtgac
     5281 cgacgccctc gtcggagccc ggacactcgt tgggggcccg ttggccgcag acctgaccca
     5341 gctcgatcgt gcgctcggag cgggagcgtc gctggccgac gcgctcgttg cctggcgcga
     5401 cgaccgaagc gcgccggcgc tcgaccagtt cgtcgcagcg gtactcctcg gcgcggagtt
     5461 gggcagccga gctgcgcccg cgttcgaagc ggtcgccgcg gccctccgcg atcaggccga
     5521 ggtggccgac gaggtccggg cgctgacgac gcagagccgg gcgtccgcga cgctgctgac
     5581 cggcctgccc ccgttggcgt gcaccggcct cgccgtcatc gagcccgcga cagctcgctt
     5641 cctgttccga tcgtcggcag gtcttgcttg tgtcggcacg gcaggagcgc tcgtcgccgc
     5701 cggctggtgg tggatggacc gactctgtcg ggccgagcgg tgagccccgt ggtggtcgtg
     5761 atgtggtgcg tcgtcgccgg cgtcggcgcc tggtggttcc gtccgcggac gtcgcgaacc
     5821 gtcgggccgt tgcatccggg cggggcggat gctccggtcg gcgggccctc gctcctggat
     5881 cgggtcggtc ggtcggtgac ggcggtcgtc ccggcactcg aaccggtccc ggcgcggcac
     5941 gtctcgctga tcctgctggc gggcgtcgtg tccgctcctc tggtcggcgc ttggtcggcg
     6001 gcgctcgttc tggtggccct ggcatgcgct cgcggcgccg gtcgacgtcg ggagacgcgc
     6061 caactcgatc gcgacctcac cgcggtggcc acgatcgcgg acctgctcgg catcgccctc
     6121 ggcagtggct tggcgctcgg accggcaatc gtcgccgtcc aacagcgact ccgaccgggc
     6181 gacgatgccg gcctcgccgg cgtcgtcgtc gacctcgagc gaggtcgatc ggtcgacgag
     6241 gcgttggacg cctgggcagg ccgcagccaa cccgtcggcg aactcgccgc gctgctgcgc
     6301 ggtgccggtc ggagtggcgc cgcgatcgtt gacgccctcg cccgactcgg caacgacgtt
     6361 cgtcgccgac gccgacgacg cgccgaggag cgggcgcgtc gtctccccgt cgccatgctg
     6421 ctcccgttgg tcacgtgcgt ccttccggcc ttcggtctcg tgaccgtcgt gccgatggtc
     6481 gccgtcggtc tcggtcggct cggcccaccg gtctgacgcc tcgagatcct cctcgtccgg
     6541 tgccgagccc acggcgccga ccccttcttc ttcccccacc atcccgacac ccaccacgtc
     6601 agaggtcgtc atgttccgat tccctttcct gcaccgtctc gccgcgctcg aacgcggcca
     6661 gagcaccgcg gagtacgcgc tcgtgctcgt cgctgtgacg tcgatggttg ccgtggtcat
     6721 cgcatacgtc agcggggacg gtggtggcct catcaccggt ctgttcggtg ccgccttcgc
     6781 caagatgcgg tcgctcgtcg gcggatgatg ccgatcgcga cgctcccacc tcggtcggac
     6841 cgggctcagg cgaccgtgga gctggcgttg gtgatgcccg tggtcgcggt gctcgtcctc
     6901 gttgtgctgc aggtcgtggt ggtgatgcgc gattcggtcg cgctcaccgc cgcggcgcgg
     6961 gcggcggcgc ggcgggccat ggtcgacccg ggtccggcgt cggtccgagc ggcggccgtc
     7021 ggtgagacga ggttgtcgcc cgcccgcctg agcgtgtcca tcggcgggtc caccgaaccc
     7081 ggtggctacg cgacggtcac ggtccggtac tcgtcgccga ccgacgtgcc gctggtgggc
     7141 gcgttcgtcg gtgatgtcgc gctctcggag cggttcgttg tgctccggga atgagggcgg
     7201 ggtgggggga caaccaatca gggagggttc ggcggtagcc tcgccctgtg tactttcacg
     7261 tccgccgtgg ggagcagggc cgctggacga tggtgcacgt cctcggggag atcgatctcg
     7321 ccacggcccc gcagtaccgc agcgagctga ttgccgccgc gtcggcgtcg acggcgctgg
     7381 cgatcgacct gaccgactgc gacctcatcg actcggtcgg cctcggcgtc accgtcggcg
     7441 gcgcccgccg gatgtggggc gccggcggcc agttcgccgt ggtgaccaac gaccggatcc
     7501 gccggacgtt cgaacgatgc cgtctcgacg agatcctcga tctcgtcgac gcgccggagg
     7561 cgttgccggc ggcatgattc cgacgcacac gaccgagctg gacatccccg ccgagagccg
     7621 gttcgtggcg acggcgcgca acttcgtggc cgacgcagcg cggcacggtg gctggatgga
     7681 cgaccagcgg ctcgacgatc tgcggctcgt ggtgtccgag acggtgacca acgcgttgca
     7741 cgcccaggcg accagcagcg tcgacgagtt gatccacgtg gtggccgccg tctacgccga
     7801 ccggatcgag atcgaggtca ccgactcggc gggcggcttc gaaccccccg acgaggcgcc
     7861 ggccctgccc gagcccgacc tccgccgtga aggcgggttc ggcctcccgt tgatcgaggc
     7921 gctgtcggac gaggcatcgt tcacccgcac gccgtcgggc acgcgggtcc gggtcgtggt
     7981 gttccgcgat cctcccgccg cctgaccggg cggcgctcca agcccttcga cggctacagg
     8041 cccggtcggg tgcgtggttc gtggaagacc cggtgcgcca ccatcatcgc cgccccggcc
     8101 gcggccacgg cgaggagcgc cgtgatgagg atcggtgtcg gcgggaggtt gagcgcgaag
     8161 aagtcgcgtc ccatcggggt gacgaaggcc agcgcgacgg ccccgcccat ggcgatcacg
     8221 agcgccaact tccagccgac gagcgggcgg gcgttgcgca gcagcacgat caacgaacag
     8281 ccggccagcg tcatcgtggc ggcggtctgc tgttccatca gcgcgaggct ctgcggatcg
     8341 gcgggggtgt agtggcgccg ggccaccacg tacgtgacga gggtggcgac cgcgcagatg
     8401 acgccgaacg ggaccgagtc gcgtgcgact cgccggagga agtcgccacg cacccggtcg
     8461 gtgttcggcg ccagggccag gaggaacgac ggcacaccga tgcacagggt gctgatgagg
     8521 gtcagatgtc gcgggaggaa tgggaagtcg agcgccagcg ctccggtcac gaaggcgaag
     8581 atcacggcgt aggtcgtctt ggtgaggtag aggccggcga cacgctggag gttgttgatg
     8641 acccgacggc cctcggccac gacggcgggg aggccggcga acgaactgtc gaggaggacg
     8701 atctgggcca cggcgcgggt ggcctccgac cccgaggcca tggcgatgcc gcagtcggcg
     8761 tccttgagag ccagcacgtc gttgacgcca tcgccggtca tcgcaacggt gtggccccgt
     8821 gactgcaacg ccttcaccat ctcccgcttc tggtgggggc tgacgcggcc gaacaccgtc
     8881 gttgcctcga ccgcttcggc gagtgcctcg gggtcgtcgg gaagggtgcg ggcgtcgacg
     8941 gccgactcgg caccggcgag gcccgcccgt gccgcgacag cggcgaccgt cgccgggttg
     9001 tcgcccgaga tcaccttgag cgtcaccccc tgctgggcga agtactcgag cgttgcgctg
     9061 gcgtcggggc ggatcttgtc ggtgagcatg acgagggcga cggcgcccag gtcgtcgggg
     9121 agcccgtcgg cgtcagcgtc gatcgcctcc gaggagtgtg cgaggagcag gacgcggagg
     9181 ccgtcttccg ctgcagccga cacgcggtcg gcaacggcgg cgaacgccga ctcggccagg
     9241 atcatctcgg gtgcaccgaa cagccaccaa ccgtggtgct cgaacccggt ggcgctccac
     9301 ttgcgagccg acgagaaggg aacgccgccc acgcgggtcc agtcgagcgg cgcttcggcg
     9361 tagcggtcgg cgagcgcctg ttgggtcgga ttcgggttcg ggtccgccca cgcgatcgcc
     9421 gcgagcgcgt tgtcgatcga ggtcgcgtcg tactcgtcgg agacgcattc gaggcggtcg
     9481 accaccatcg tgccttcggt gatggtgccg gtcttgtcga ggcagaccac gtcgacccgg
     9541 gccaggatct cgaccgccgg gagttcctgc acgagggtcc gacgcttggc gaggcgaacg
     9601 acgccgaccg cgaacgccac gctcgtgagc aggaccaggc cctcgggcac catgccgacc
     9661 atgccggcga ccgcggcgac gagcgcgtcg ccgaccgact tgccgggctg gcgcagctgc
     9721 gaccacacga gcccgatcgc tgcgggaatc agcgcccacg acacccaggt gacgatcttg
     9781 tcgatcgagg tccgcagctc gctgttggcc agcgagaaac gtcgcgcctc ctcggcgagc
     9841 ttcgcggcgt aggcatcggc gccgaccttg gtgactcggg cgcgcccgga cccggtcacg
     9901 acgaagctgc cggagaagag ctcggagccc ggcgacttca cctcggggtc ggcctcgcca
     9961 gtgaggagcg attcgtcgat ctcgagcgcg tcggactcga tcacctcggc atcggccacg
    10021 acctggccgc cgggccggag ttcgatgacg tcgtcgagca cgatgtggtc gacgctcatc
    10081 tccacgacct tgccgtcgcg gacgacgtgc gccgccggcg ccgacagcac tgccagctga
    10141 tcgagtgtgc gcttcgccct gacctcctgg atgatgccga tcaccgagtt ggcgatgatg
    10201 acgccgccga acagcgcgtc ctggggcgaa cccgacgcga gcacgatcac cacgagcagg
    10261 ccgatgaggg cgttgaacct cgtcagcacg ttggcgcgca ggatctcggt gaccgtccgc
    10321 gacggggcgg gcggcacctc gttgacgagg ccgcggcgta cgcggtcctc gacctcggcg
    10381 gcggtcaggc cgtcgcgggt cacctgggcc ggtaccggaa cgatcgacga cgagcctcag
    10441 aggtgaccgg tgtcatcagg agtagcgccc cagctcggtg cgagcgacct ggcggcggtg
    10501 gacctcgtcg ggcccgtcgg cgaaccggag ggtgcggagc ccggcgtaca tgcgagcgag
    10561 tgggaagtcg tcgctcactc cgccgccgcc gtgcgcctgg atggagcggt cgacgactcg
    10621 gagcgccatg ttcggcacga cgaccttgat cgacgagatc tccacggcgg cacccttgtt
    10681 gccgacggtg tccatcagcc acgctgcctt gaaggtgagg aggcgagcct gctcgatctc
    10741 catgcgggag tcggcgatcc actcgcggat gaccccttgt tcggccagcg gtcgaccgaa
    10801 cgccacacgg tcggtgacgc ggcggcacat cagctcgagc gcacgctcgg cgatgccgat
    10861 gcagcgcatg caatggtgga tgcggccggg cccgaggcgg gcctgggcga tcatgaaacc
    10921 ggccccttcc tcggcgatga tgttctcgac cggcacccgg acgtcctcga accggagctc
    10981 gacgtggcct tcccggtcct ggtagccgaa cacgggcagg tcacggacga cggtgaggcc
    11041 gggcgtgtcg cgggggacga ggatcatcga ctgctggagg tgacgcgacg ccgtcgggtc
    11101 ggtcttgccc atcacgatga agatcttgca gcggtccgac gctgcgcccg agatccacca
    11161 cttgcgcccg ttcaggacgt actcgtcgcc gtcgcggacg atggagagct ggatgttggt
    11221 ggcgtcggag ctggcaacgt cgggctcggt catggcgaag gccgaccgga tctcgccctc
    11281 gagcagcggc tggagccact gctccttttg ggcgtcggtc ccgaacatcg tcaggacttc
    11341 catgttgccg gtgtcgggcg cagcgcagtt gagggcctcg ggcgccaggt ggatcgaccg
    11401 accggtgatc tcggcgagcg gggcgtagtc gaggttcgac aggccttcgg tccactgggt
    11461 ctcgtggggg aggaagaggt tccacaaccc gcgggtgcgg gcctcggcct tgaggtcctc
    11521 gacgatctgg ggatgggcgt gggggtcgcc gagggcggcc atctgctcct cgtagaccgc
    11581 ctcggcggga tagacgcatt cgtccatgaa ggcgagaagc ttgtcctgca acgcgagagc
    11641 ggtcgggctc agttgaaaat ccatggcgtc tccggagtgg gtcgttcgtg cgcgatcggc
    11701 caggtgccga acgcggcttg gcccatcagg acagtatcgg ttgcgccgtc gacggcgacg
    11761 cttcgggccg gcgcggagtg gacgcgccga ttcacctttg ttctggggat gtttgacacc
    11821 gctacacgtc ggcgtcttga gtgtccccca tgggcaaccc cctcgtcatc gtcgagtcac
    11881 cggccaaagc gcgcacgatc gggcgcttct tgggcgacga cttcgtcgtc gagtcgtcgg
    11941 tgggtcacat ccgcgatctc ccgtcgaagg cggccgagat cccaccttca tataaaggtg
    12001 tgaagtgggc gagcctcggt gtcgacaccg agaaccactt caaaccgctc tacgtggtgt
    12061 cgcccaacaa gaaggaccag gtgcggcacc tgaaggagtt gttgaaggac gccgacgagc
    12121 tctatctcgc aaccgatgag gatcgcgagg gagaggccat cgcctggcac ctgctcgagg
    12181 tgctcaaccc ccgcgtgccc gtcaagcgca tggtcttcca cgagatcacg cccgacgcga
    12241 tccaacacgc catcgacaac ccccgcgagc tcgaccgtcg cttggtcgac gcgcaggagg
    12301 ctcgacgcat cttcgaccgc ctctacggct acgaggtcag tccggtgctg tggaagaagg
    12361 tcatgccgcg gctctcggcc ggccgcgtgc agtcggttgc gacccgcatc gtggtggagc
    12421 gggaacgtga gcgcatgcgg ttcgtcaccg cctcgtactg ggacctcgac gcgaccttcc
    12481 agctgaccgg caacgacccc accggtgccc cgagggacca gacgccgttc gccggctcgc
    12541 tcctcgaggt cgacggcgcc cgcgtcgcga ccggcaagga cttcgacgaa tccggccgcc
    12601 tcaccaaggc cgacgccctg ctcctgagcg agaccgacgc cgcccagctc cgcaccgcgc
    12661 tcaccgatca gcccgtccgt gtgcggtcgg tcgaacgcaa gccctacacc cgcaagccgt
    12721 acgcgccgtt catgacgtcg acgctgcagc aggaggccgg tcgcaagctc cgcttcagct
    12781 cgagccagtg catgtcgttg gcgcagcgcc tgtacgagaa cggcttcatc acgtacatgc
    12841 gaaccgactc gacgacgctg tcggagacgg cgatcaacgc ggctcgttcc cagatctccg
    12901 agaagtacgg cgctgcgtac ctccccgacg ccccgcggct gtactccaag aaggtcaaga
    12961 acgcgcagga ggcccatgag gccatccgcc ccgccggcga tacgttccgt tcgcccgacg
    13021 aggtccggtc cgaactcggc agccaggagt tccgcctcta cgagctgatc tggcagcgca
    13081 ccatcgcctc ccagatgacc gacgcccgcg gcgagagcgt cgccgtccgg ctcgaggtcg
    13141 acgcgaccga cggtcgtcag gccctgttcg cgtcgagcgg caagaccatc acgttccccg
    13201 gcttcctgcg ggcctacgtc gagggcgccg acgatcccga cgccgagctc gaggaccagg
    13261 aacgcatcct gcccgagatg gccgaaggcg acgccgcccg atcactggag ctcgcggcca
    13321 agggccacga aacgcagccg ccgccgcggt acaccgaagc gtccctcgtc aagcgtatgg
    13381 aggagatcgg cgtcggccgg ccgtcgacct acgcgtcgac catctcgacg atccaggacc
    13441 gcgggtacgt gcgcaagaag ggctcggcgc tggtccccag cttcgtcgcc ttcgctgtcg
    13501 tcaccctgct ggaacggcac ttccccgacc tcgtcgacta cgccctcacc gcgcacatgg
    13561 aggacgacct cgaccagatc gccggtggca aggcggacgc cgagccctgg ctctcccgct
    13621 tctacttcgg cacgaccggt gacggcgatc cggcgtcggc acggcccggc ctcaaggcgc
    13681 tcacctccga catcgagggc atcgacgccc gcgacgtcaa ctcgatcgtg ttgggcgcgg
    13741 atgccaacgg tgtgccgatc gtcgcccgtg tcggccggta cggcgcgtat ctccagcacg
    13801 aggacgagac ggcgaacatc cccgacgaca tctcgccgga cgagctgacc gtcgagaagt
    13861 gtctcgagct gctggcaacc ccgaaggaac gccagctcgg catccacccg accgagaacc
    13921 tgccgatctt cgtgaagaac ggccggttcg gtccgtacgt gcagctcggt cagcacgacg
    13981 acgagaccgg tttcaagccg aagatggcgt ccctcttcaa gacgatgacc gtcgagcgga
    14041 tcacgctcga ggacgcgctc gaactgctga gccttccgcg ggtggtcggc gtcgaccccg
    14101 ccgacggtgt cgagatcacg gcacagaacg gcaagttcgg gccctacatc aagaaggaca
    14161 ccgactcccg gtcgatcgag gacgaggagc agctgctcac cctgaccctc gacgaggcgc
    14221 tgcggatcct ggccgagccg aagcggcgcc gcggtcaagc ggtcaagggt ccgctcaagg
    14281 aggtcggccc cgaccccgac agcggcaagc cgatcgtcgt caaggacggc cggttcggtc
    14341 cgtacgtgac cgacggcgag accaacgcct cgctgcgcaa gggcgacatc gtcgaggagc
    14401 tcacgatgga gcgcgccctc gagttgttgg cggaccgtcg agccaaggcg cccgccccga
    14461 agaaggcggc agcgaagaag gcccccgcga agagggcgcc agccaagaag aaggctgccg
    14521 ccaagaaggc ccccgccaag aagaaagctg cggccaagaa ggcgccggcg aagaagtcgg
    14581 cgaccgccgc cgtcaccccg aaggccgtcg ccgacgcctt catcgcctcg gccggcgacg
    14641 agccgttctg acaggtgagc acgggtcggt cgcagtgacg gcgaccctcg gtgagcctct
    14701 gcgcgtccgt tggttcgggt cgatcgagtt cttctggctc tggatctccc agctgatcag
    14761 cgcgttcggc gactgggtcg ggctcttcgc cgtgaccgcc ctcgcagcga acatcagcaa
    14821 tcgtccggag gctgcgaccg cgctggtgct cacggctcgc gtcgctccga gcttcttcct
    14881 cggcccgttc atgggggtcc tggtcgatcg gtacgaccga aagctgctga tgcggatcgc
    14941 ggacctggcc cgggcactgg tgttctgcgc cctgccgttc gcgacgacgc tgtggcacct
    15001 gatcgtcgcg tcgctgctgc tcgaggtgtt cacgctgctc ttcgccccgg cgaaggaagc
    15061 gctggtgccg gtattggtcc cgcgtgaccg tctgacctcg gccaactcgc tcggcattct
    15121 cgccgcctac ggaacgatgc cgctcgccgg tgtcgcccag acgctgttga aggccggcaa
    15181 cgaccggctc gcccatgtcg agtggctcgg tcccctcggg ttcggtcgcg atatcgggag
    15241 cacccagacc ctcgccttct acttcaacgc gctcacctac gtggcggcgt tcgtgatcgt
    15301 ccgcacactg atccgcacga aggccgagcc ggttcacgag cgcgtcggta ccgtcgagga
    15361 aagcgacgcg ccgtcgggca tccgggccgt cctggcggag gtccgcgagg gatggaagga
    15421 ggtcgtcgtc aacccgatcg tgcgtgccgt gaatgtcggg ctggccgccg gcctgctcgg
    15481 tggcgccatg ctggtcccgc tcggacccac gttcgcgaag aacatcgtgg gcgacgtcga
    15541 caagttcccg ctctacatca cggcgttggg atgcggtgtc gccttgggtg tcggtctcct
    15601 caccgccttc cagaagaagc tcccgaagga gaaggtgttc tgtggagcgc tcttcttcgc
    15661 gggcggctcg atcctgttcg gcatcagcat gtcgacgttc tggctatcgg cggtcggggt
    15721 gttcggtctg gggttgggcg caggagcggt gtacgtgctg ggatacaccc tcttgcagga
    15781 gaacaccgtc gacgacatgc ggggccggac gttcaacacg ttcctgacac tcgtacgcct
    15841 ggccgtcctg gtcgcgatgg tggccggccc gacgatctcg tcggtgctcg acccactgat
    15901 gaagcggtgg atcggcggcc gcaccgcgtt gggtacgccg ggtgtgcgat ggttcggcgt
    15961 ggagtacgga ttgccgggcg ttcgcgtcac gctgtggttc ggcggcctgc tgatcctggt
    16021 cgccgcgacg ctcgcgtcgc gcagcatcga tccgtcggct gttcgcgtgg gacgtgcccg
    16081 acggcgcgac accgtggcag ccgggtctga ggaactgaca tgaccgggcg gttcatcgtc
    16141 ttcgaaggcg gcgagggttc gggtaagtcg acgcaggcgg cgatcctcgc cgaccgcctc
    16201 gacgccgtcc tcacccgtca gcccggcggg accgacatcg gggcggcatt acgcgccatc
    16261 ctgctcgaca ccggcacggt cgccctcgcc ccgcgcgccg aggcgttgct gatggctgcc
    16321 gaccgtgccc agcacgcggc cgagctgatc cgccccgctc tcgccgccgg ccgacacgtc
    16381 gtgtgcgacc gctacacgta ctcgtcggtc gcgtaccagg gccatggccg gggtctcgat
    16441 ccgcagcggg ttcgcgacat ctcggcgtgg gcgaccgagg agctgtggcc cgatctcgtg
    16501 gtgctgctcg acgtcgaccc cgatgccgcc gcaacccgct tggtccgcga actcgaccgg
    16561 ttcgaacagg ccggcgacga gttccaccgc gccgtacgcg acggcttcct ggcacaggcg
    16621 ctggcggagc ccgagcggtg ggtggtcatc gacggcaccg ggaccgtcga cggggtcgct
    16681 cgtgccgtgt gggcggccgt cgaacccgtg atggagtccg agtgatggag cgagcatgac
    16741 cgagacggcc ctggagacgg agctcgacct ctgggaggca gtcgtcgggc aggacgagat
    16801 cgtcgcgcaa ctgcgatccg ccgccgccga cccgacccac gcgtacctgt tcgtgggtcc
    16861 acccggagtc gggaccatgg ctgcggcacg ggcgttcgcg gccgagatcc tcagccgggg
    16921 tgccaccccc gagcaagcgg agcggcaccg tcgcctcgcg gcggccgatc atcacccggc
    16981 ggtcatcgtc gtcgagcgcg tcggcgcatc gatcctcgca gcgcaggcgc gtgagatcgt
    17041 ccggcaggca cagatgcgcc cgcccgaagg cgatctgcag gtgctgatcc tgaccgagtt
    17101 ccacctcgtc accgtcgccg caccgatgct gctcaagtcg atcgaggagc cgcccgccgg
    17161 cacgatcttc atcgtggtcg ccgacgaggt cacccccgac ctcgtgacca tcgcctcgcg
    17221 gtgcgtgaca ttcgagttcc cgcacctcag ccgcaaggtg atcgaggaac gccttcgtct
    17281 cgaaggcgcc cccgccgacg tcgccgaggc agcagccgtc ggatcgggcg gcgacctggg
    17341 ccgcgctcgg ctgctggcga ccgacgagca ggtcgtcgac cgccgcaact tctggttctc
    17401 gcttccggag cggctcgacg gcacgggctc ggccgctgcc cgcctggccg ccgaggtgat
    17461 ccgccgcgtc gaggaggtgc tcgcaccctt ggcggcgatg caggcggcgg aaatggagga
    17521 gctcgtcgaa caggccgaga agctcgggca gcggaagacc gcgctgaagg aagtcgaggc
    17581 gcgccacaac cgcgagaagc gccgcatccg gatggacgag ctgcgcgccg gcctcgccgc
    17641 gttgatggac gggtaccgca cggtcgcggc gaccgagccg cagacctacc tcgacgctgc
    17701 ccgtctggtc ggcgaactca ccgatcacct ccagttcaac cccaacgagg agctggcgtt
    17761 gcaggccctc ttcgtgtcgc tcccgcggcg gcggtagccg gggtcggtca gacgctgctt
    17821 tgcagggtgg ctgcttcggc ggccttggtc gcagtcgccg cggcaacgca gagatcgtgg
    17881 accaacgaca gcttctcgac gacgttgggg cgcagcacga acgggtagag ccggtccttg
    17941 cccatcgccc ggttgatctc gttgagggcg taggtcaggg gaacccagtc cttcatcagg
    18001 acctcgaagg acgcgttcgc gaccgcgtgg gccgggtcgc tccgaaaccg gttcgacgtc
    18061 gggctgttgc gaagggccgc agggccctcg atgtagacgc cgtacgcggc cgccgtctgg
    18121 agcgtgccgc ggatgtggag gtagtgggcg aacgtctcgg cccagtcctc ccaggggtgg
    18181 gtggtggcgt agttgctgac gtagtgctcc tcccaccccg gcggcgggcc gccggcgtag
    18241 tgccggtcga gcgcagcctg gtagtcggcc gtgtcgtcac cgaactgctc gcggatgcga
    18301 gccaacacgt cggggtcatc ctgggtgaag agcatccagt agtagtgacc gatctcgtgg
    18361 cgcagatggc ccaacatcgt gcggtacgac tcgcccatcg tcttgcggag ccactcgcga
    18421 tgggcgtcgt cggactcggc gagatcgatc gtgatgacac cgtcggcatg accggtggtg
    18481 acgtgggtgt agcgactcga cagcaggtcg aacgccagcc cgtgggcggg gtcgacgctg
    18541 cggctcacga ccggcatccc cagcgagtcg agctggaaga gcaggcggcg cttggccgcc
    18601 tcggcaacgg tcaacgcgtg ctgcgcctcg acgtcggacg cgtcgggcag cgtccgggtc
    18661 agctcgcagc tccgacaccg gtcgccgggt tcggtatcgg gcacgagcca gttgcacatc
    18721 gtcgtggatg cgttgacaca tcgacgccac cagatgccct cgggatcggt gaaccgccgg
    18781 taccgggacg gatcgtggtc gtcgggttga agcgtgatga caccgctgcc ggggatgacc
    18841 ccgagcggcg cgaggcatgc caggcacgcc gtgttgtcga agaagaccag ctgtccgcag
    18901 ttgtcacagg tgaacgcgag cacgtccgac ggtaccccgc atcggccgca tcgaacgcgc
    18961 tcggagggaa ttcccaggag caacccagcg cgcccccagt ctgggcggcg atcctggcgg
    19021 tcgtgaaccg ccgtgtcctc atcgtcgatc ccgacccgct cacccgcgag ttgcatcgga
    19081 tggcgctcac gttcgtcggc cacgacgtca gggtcgtcgc gtcgagcaac gacgccgagt
    19141 tgttgttcga cgggttcggt cccgacgtcg tggtgctgac cgtcgacatc ggaccggagg
    19201 acgcgacccg gttccgggac cgtctcgtac ggtcgggcga ccccgtacgc caggtcgtga
    19261 tcgtgggcga tcaggccgat cggcgccgcc ctggcgccgc ggtcgttctg gcacgaccgc
    19321 tcgatctcga cgcgctggtt gcagcggtcg agggaggcac gggcggcgag tcgacggtgt
    19381 cggtcgggac actcgagctc gatcgccgtc gcgagcgggt cgtcgtcgcc ggacaggagg
    19441 tgcacctgtc gccgacggag ttccgcctgc tgcgtctcct ggcgatcaac gccgagcggg
    19501 tcctgtccaa ggccgacatc ctcgaccagg tgtggcgcta cgacttcgga gggcggacga
    19561 acgccgtcga gacgtacgtc agctacctcc gccggaagct cggcccgctg ggcgggccga
    19621 gcatcgtcac cgtgcggggt gccgggtacg tccttcgggc gtagcgcccg gctcaggcgg
    19681 ccaggtcggc gagttgaccg gtcgagagcg acgccaacaa ctgctcggcg gtgaacgcct
    19741 ggtacgcccg ttgtgcaccg gcggtggcac tggcgcccgt tgtggacgtg ttgtcgtcct
    19801 cgatcgacgt gccgggcggg ggaccgtggt gccctcgggt gtgacccgcg ccggtgtcga
    19861 ctccacggag cccactggcg gtcaggtcgg aatcggcgcc accagcggcg ccaccggtcg
    19921 gtgggggcgg ctgcgtgccg tcgatcaggt gcgtggcgaa ctcggtgatc tggtcggcgc
    19981 tgagctgtcg tccgtgcttg gcggcaccgg cggcgagccc gtcggtcact gcggcaagga
    20041 ggtcgtcctt cgacacgccc ttcgacgtgg ccaggtcttc gagggtcgaa ccgccggcga
    20101 gcgcgtctcg gacgtcggac tcgctcatgt cgagcgcctt ggcggcggat tcgattgcct
    20161 cgggtggggg ccccggcggg cggtgcccgc cggtacgacc cgaccgagct gtctcgtcgg
    20221 tgttcgtcga gctcgtagcg gtcgaactca gtcgttgcag ggtggccagg tcgagagcgc
    20281 tggcaccgga ggtgatcgag ctgatcatgg gatgtggact cctggagggg gaagcgtcgg
    20341 gctggcccac cgactggttc gtgtcaggac atggatcggc cgggacgcac cggcgtccga
    20401 atggttcaca ggaattcaca ggttgcgtcg ttaccctgga tgccatgcgc gtccgcttca
    20461 tccccgtcct cgctgcgtcc ctcctgctca ccgcgtgtgg cggtggtgac gacggagcca
    20521 tcacgatcgg atcgtcgaaa gcgcccggtg gtgccgcgac gttcgacgcc gaccagtgcg
    20581 ccgtcttcga gtccgaggac gggccggacg agaagttcgt cgagtcgatg ccggaccggt
    20641 accgggacgc ggcgacggcc atcgccgagt tcggcacgtc gatggacgcg atgggcgaca
    20701 acgacgacgg ctcgatcgac ggtctcgtcg cgtcgctcac gaaggacggt gtcgcggagc
    20761 agctccgcga gttcgccgcc gacatcgagg agcagtgcgg atcgagtgag gggtcggcgg
    20821 tgatcggcgg gatcgcgacc gcgtcggaga tggcgtcggc accgaaggat gccgactact
    20881 gcgacggcat cgaggcggga ttctcgaccg acgacggggg cgccgaggag atcgcggcac
    20941 tcgccgacat cgctccggag tcacatcgcg agccgctcgc agcgttggcg gcggtggacc
    21001 ccgagtcgat ggacagtgct gatcaggacg ccctgttcgg cccgttgttc ggcctcggga
    21061 tctacgccga ggaccagtgc ggtatcgaag gtgcgctcgc ccagatgctc ctgggcgcca
    21121 tgttcctcgg tgccggcgac ggtatgacga cgaccacgct cgccgaggga gtggacccgg
    21181 cgcagtaccc cgacatcacc gccgatgcgg ccaacgctgc gcttcctgcg gggagtggta
    21241 tcgcgttcca ggtcgtcagc gccgacctcg acgacgacag cgaatacctc gcgtcgatgg
    21301 tcgtgcccga aggttggacg agcgacaccg ggttcaacgt cgagttctcg ccgcccgccg
    21361 actcgggtat cagcatcttc acgtcgatcg aggcgggggc cggttgcgac ggcacgtgcg
    21421 aggcaaccga ttgggacgag cgcctccgtg gcgagtccgg attcctgacc cagttcttct
    21481 cctcccatcc gggagcggcc gaggcgccga tcgccggatc ggagggcgtc gtcgtcacga
    21541 actcgaccga ggacgaggcg gtggccttgg tgctgcggtg ggacgacggt gccgacaagt
    21601 acttcagctg cgaggtgtca ctcgagcccg agcaggccga tctcctcccg gcgttcgtcg
    21661 ccgcctgcga gtcgtcgcgc ccggcctggt tcgccgtcgc ctgacccgcc gttcaggcga
    21721 ggagggccag gcgtcgcgga ccgcggacct ggccgaccga ccacgtcacg gccgacgggt
    21781 ctgcgagacg gaactcgggt acgcgggcga gcagacgttc gccggcgatc cgcacctcga
    21841 ggcgcgcgag gtgtgaaccg aggcaacggt ggatgccgac gccgaacgcg agatgacggt
    21901 tgggctgccg gtcgaggcgg atcgcgtcgg ggtcgtcgaa cgcttcggga tcccggttgg
    21961 ccgccgggaa cgggagcagc accgggtcgc cctggtgcat gggacaccca cccaacgtga
    22021 cgtcctcgac cacctctcgg gccatggtca ccggggcgta ggcccggagg aactcctcca
    22081 gcgcgtcgga ccacagcgcg gggtcgtcgc ggagctgacg ttgttcttcg gggtgctggg
    22141 ccaggtgcca gatcgtcgat ccgatcgcgc tccacgtggt gtcgatcccg gcgatcagca
    22201 agaggaggca tcccccgaac acctcgttgt cggtcagggg gcgcccgtcg agttcggcgc
    22261 tgatcaggaa gctgatgagg tcgtcgccgg gtgccgtgcg gcgctcggcg atctgggcct
    22321 cgaagtacag gtagaactcg gtgagcgcgc tgatcgctac atccagatcg gtcggcgcga
    22381 gctccagcat ctcgtgcacc cacttccgga acagctcgcc gtccgcgtcg ggcacaccga
    22441 gcatgcgggc gatcacgtgc accgggatgt gctgcgcgta ctgctgcgcc gcgtcgaagg
    22501 agatcgtgcc gccgtcgccg gcgaagtcgt cgatgagtgc gtcgcagagg tcggtgacga
    22561 tcgggcccat cgcctcgatg cgtcgcggcg agaagaacgg gagaaggagc tggcggaacg
    22621 cggtgtggta cggcggatcc gacgagatcg gtggcaccac gaagccgaag tcgctcatcg
    22681 aaccgaactg tgcgaccagc gggctgcgcg acgagaagtg tgtggtgtcg cgggcgatgg
    22741 cttcgatgtc ggcgtgtcgc gtcggtaccc acatgccgcg gaagcgctcg gaccgggcga
    22801 tcgggcagtc ggtgcgcaac gccgaccaga cggtgaacgg atcggcggcg taggcggggt
    22861 cgtagtgatc gaatcgggtg tgccagtccg tcgatgcggg atccttgacc attggtcaaa
    22921 cgtagaaccc gattgaccag tggtcaagta ttgtggcgcc cgtggcagcg acgacggaac
    22981 gggcgaacga ccgcgccaat gtcggcgctc gcagcgatcg cagcgctgag ctcgtcgcgg
    23041 ctgcgatcgc cgtcgtggcc ggcggctccc tcgacgcgct cgacgctgcg tcggtggctc
    23101 gagaggccgg agtctcgaag gccctcgtgt actactactt cccaacccac ctcgatctgc
    23161 aggccgccgt cctccggagc gcagccgagg atctgctgga ggcgatcgcc cacgccgtca
    23221 ccgcagcggc gccagcggga acgccgatca ccgcggggct ggatgccgcc gtcgcgtaca
    23281 tcgaacgtca ccccgccgcc tacgtggccc tcgcccgcac cgccggctac caccccaagc
    23341 tgtcggaggt gttcgagttc gctcgtgacg gtgtggtcga cctcatggcc acggcgttcg
    23401 gcatcgacga gccgacgcaa cgccagcgga tcgccctgcg cagttggatc gcgctcgccg
    23461 aggaggccgt cctgcactgg atcgtcgccg accgtccgat cgagcgatcc gagatcgtcg
    23521 actactgccg agcggtggcc gaattcgtgg ccaccacgcc gctcgcccgg tgatgcgatg
    23581 agcgggccac tgcacgacct gcgggtcatc gagatgggtc aattgctcgc gggaccgttc
    23641 tgcggccagc tcctggcgga cttcggtgcc gacgtgatca agatcgaacc gccgggtcag
    23701 ggcgacccga tgcgggagtg gggccgcgag aaggccggcg gcacctcgct gtggtggccg
    23761 gtcgtggccc gaggcaagcg ctcggtcacg ctcaaccttc gtgaggccga gggtcaggag
    23821 atcgttcgcc ggctcgtcgc cgatgccgac atcctcatcg agaacttccg tcccgggacg
    23881 ctcgagcggt ggggcctcgc cccgagtgac ctcatggcgc tgaacccgcg gctgatcgtc
    23941 gtgcgggtgt cgggctacgg ccagaccgga ccgtacgccg accgtccggg gtacggcgcg
    24001 atcggcgagg cgatgggtgg cctccgcggc gtcatcggcg aacccgatcg gccgccggcg
    24061 cgagcgggca tctcgatcgg cgacacgctc gccgccatct tcgcctgcgt cggcacactg
    24121 gtggccctgc actcgcgttc gaacaccggc gccgggcaga tcgtcgactc ggcgttgtac
    24181 gaggccgtcc tcggcgtgat ggagtcgctg atccccgagt acacgatcgc cgggtacgtt
    24241 cgacccagaa ccggctcgat cctgcccaac gtcgccccgg cgaacgcgta tccgaccgcg
    24301 gacggcgaac atctgatcag cggcaaccag gacacggtgt ggcggcgact ggccgtcgcg
    24361 atgggccgcc cggagctcgg cgacgacgag cggttcgcca cccacaacgc ccggggcgaa
    24421 caccagagcg agctcgacga cctcctcggc gactggaccc ggacgctcac gtcggccgaa
    24481 ctcgaagccg tgctcaacga gcactcggtg cccaacggca agatctacac ggcgcccgac
    24541 atgctggccg atgcgcactt cgccgcccgc gaggcgatcg tcacgatggt gcacccgttg
    24601 ctcggcgact tcccgatgca gaacgtcgtc ccgaagctga gcgacacccc cggcgaggtg
    24661 cgctgggtcg gacccgagct cggcgagcac accgacgagg tgctcaccga gttgctgggg
    24721 ctcgacgacg agacccgtgc cggtctgcgc gagcgcgggg tcatctgacc gcgccggggc
    24781 cgcgggaccc cggccggctc acgccttgtt gcggtacgcg cgcatcgtga gcggggcgaa
    24841 gacggcgacg agcacggcac aggcgatcag cgtcttgccg atgtcgctcc cggacgcctc
    24901 gccgtgcatg agcccacgga ccgacgtgac gagccagctg atcgggttca cgtcgacgaa
    24961 cgccttcagc catcccggca tggtgtcggg actgacgaag atgttgctgg cgaacgtcag
    25021 cgggaacagc agcatcatgc tcatgcccat caccgacttc tcggtgcgga ggatcaatcc
    25081 gaacatcgtc cagatccacg acaggctgaa cgcgaagacg agcagcaaca ggaccgacgc
    25141 caccacgccg acgaccccgc cgtcgggtcg gaacccgagg atcagaccca tcacgatgat
    25201 gacggtcgaa gcgatcgaat agcggacggc gtcgccgagc agcggaccga cgatcgcgga
    25261 cggccgccac atcggcaacg accggaaccg gtcgaagatg cccttctcgt ggtcgacgtt
    25321 gagcccgacg ccggtgtaca tcgtgatgaa cagcaccgac tgggcgagga tgcccggcag
    25381 gaagaactgc aggtacgcct tggtcgatcc tgcgagcgca cccccgaaca ggtaggtgaa
    25441 catcagcgtc atcatgatcg ggaagatcgt gacgtcgaac agctgctccg gtacgtgctt
    25501 gatcttgagc agcgcccgcc aaccgtgggt gatggagacc gacagcgcgt tggggcgctt
    25561 ggggcgctct cccgacgcga gcgccgagag gatggaatcg acatcggcgg gagcaaggtc
    25621 cggggcgagg gtgtcggtca tgagcgtgcc tcctccttgt gctgggaatc gaggggatgg
    25681 tcgggatcgg aggacgactc ggccgtgtgg ccggtgagtg ccaggaaaac ctcgtcgagc
    25741 gtcggctggc cgaaggcgta ggtcgcgatg tcgatggccg cctgcgacag cgccgacaac
    25801 cctgcggcga cccgttcggc gtcgtcgacg cgcgccgaca gcgctgccgg gtcgggttcg
    25861 agcgcgaccg ggcggtcgag gtggcgctgc aggatcgact gcgcctcggg acggcgttcg
    25921 gggtcggcga cgcggacgtg gagtgctccg gcgccgaccg aggccttgag ttcgccgggt
    25981 gtgccctcgg cgatgacctt cccgtggtcg atgacggcaa tgcgcccggc gagctggtcg
    26041 gcctcgtcga ggtactgggt cgtgaggagg acggtggtgc cggccgccac gagtgctcgc
    26101 acgatgtccc acacctggtt ccggctccga gggtcgaggc cggtggtggg ttcgtcgagg
    26161 aacagcaggt cgggggtgac gacgatgctt gcggcgatgt cgaggcgccg tcgcatccca
    26221 ccggagtagt gcttggcctg cttgtcggcc gcgtcgatga gcccgaaggc ggtcaggagc
    26281 tcggtcgctc gtgaggtggc ggcggcccct cgatggccga gcagcttcgc gaggagccgc
    26341 aggttctcca tgccggtcag gtcttcgtcg acggaggcga actgcccggt caggctgacc
    26401 atggagcgga cggtgtcggc gtcgtcgacg atgtcgtgtc cgaacacggt cgcgccgcct
    26461 ccgtcggggc ggagcagcgt ggccagcatg cggatgacgg tggtcttgcc ggccccgttg
    26521 ggcccgagaa cgccgtagac gccaccggtc gggatggtca ggtcgaggtc gtcgacggcg
    26581 cgtgtgtcgc cgaagacctt gaccaacccg cgagcttcga tcgcgtgtgg tgcagtgagg
    26641 gtgctggtca tgggtcgctc ctgtgggacc gcggtgccgt cggctccgag gttgtcagat
    26701 atgtcgacgt tctgtcttcc cgaaaatcat cgcgggcagc cgatccgtgc ccgcccgtgc
    26761 ggtgtgagtg accgtgtacc cacgtagagt gtccggcgat gcagtggcgg acgaacaccc
    26821 ttcgagagcg acggaagcgt cgcgtcgcgc tcgttgcgct cagcgcgtgc gcactgctcg
    26881 cagcgtcctg tggcgacgat cccggcaaca tctcgatcgg cccccgtgcc acgacctcgg
    26941 tcgccgagac cacaacgacc accgttgccg agacgacgac cgcgcccccg gagtcgacca
    27001 ccgccgtacc ggagacgacg acggcgccgc cgcagtcgat cgtgcccgcg accgtcgccc
    27061 cggccctccc cggtgaccat cagggcgagg gcgacacggc cgccggccca cggcccgagg
    27121 tcgtccgtcc gcccgccgcg tggatcgggt tcgggcagcc ccaacccggt tcgatgttcg
    27181 agaacctcca cgaggaccac gcactcgacg gcaagggcaa gttcgtggcc ttcaccttcg
    27241 acgacggtcc ctcgcagtac acgaagccga tcttcgacat cctcaagttc atgggggtgc
    27301 aggcgacgtt cttccagatc tcccggcagt ccctggcgaa ccaggaccag gtcaaggaga
    27361 tgctggccgc tgggatgcac atcggctccc acaccatcaa ccacccccac ctggccgaga
    27421 tcccgcccga ccagcagcag gccgaggtac tcggctccat cgaccagatg aacgcggcct
    27481 atggggccgg cacggtcaag tgcttccgcc cgccctacgc gcagtacgac cagcacgtcc
    27541 tcgacctggt gaaccagcgg ggcgctgcta ccgcgatgtg gtccctcgac acgctcgact
    27601 ggaagaagcc ggcgtggcag tcactcgtca cccgcgtcgt cgacggtgcc caggatcgca
    27661 acgtgctcct gttccacgac gggggcggcg accgcaccca gacgatcctg gccctgccct
    27721 ggatcatcca gggcctccgg gacaaggggt tccagttcat ccccgtttgc tgaatcggtt
    27781 ggccgcagcg ggggtgttcg ctggtagatt ctccctctca agcccgggta gctcagacgg
    27841 cagagcaatt cactcgtaat gaataggtca ggagttcgat tctcctctcg ggctca
//
LOCUS       DAOSOA010000011        25748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_1910_length_25748_cov_7.651812, whole genome shotgun sequence.
ACCESSION   DAOSOA010000011 DAOSOA010000000
VERSION     DAOSOA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 25748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25748
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1910_length_25748_cov_7.651812"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PKY13_01780"
     CDS             complement(<1..883)
                     /locus_tag="PKY13_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT78680.1"
                     /translation="MSNTEPSSTEPSDPALPWWKTAVGYQIYPRSFCDTTGNGIGDLD
                     GVRRHLDHIADLGVDALWLSPFFPSPMADYGYDVADFCGVDPLFGDLATFDALVAEAH
                     GSGLRVIIDWVPNHTSDQHPWFLESRSSTDNPKRDWYHWRDPAPDGGAPNNWIESLTF
                     GPAWTFDETTGKYYLHCFLSQQPDLNWGNPEVVAAMHDTLRFWLDRGVDGFRMDVVHL
                     IGKDPALPDEPPDVAGLPHTILNDRPETHALLRELRILLDSYDGDRMAVGEVYLLDTE
                     LVASHYGNGDELHLCFNF"
     gene            936..2120
                     /locus_tag="PKY13_01785"
     CDS             936..2120
                     /locus_tag="PKY13_01785"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT78681.1"
                     /translation="MGLREFTSDEAVVAGRLAALKDEAGSHSPSLLTLREQMPELEIA
                     VDACFISNPYATQLFLDYFKSELLDTGAIRDVLEFYPSQNQVIGALLAGHLGIPEDRV
                     FVGNGAIEIIQAVIHNFAKRRVLVTLPTFSSYYEFAEGRVDVVFHTLAKTDEFKLDPH
                     DYLAKVAETEPDVVTLINPNNPDGSYFSYEDLLTILRGLPDDVTAIVDESFIHFAFED
                     LNLDIRSIIAECEQFPNLVVVKSMSKDFGIAGIRAGYAVMHPDRIDALLRNGYLWNSN
                     GLAEYFFRLYCRADFLERYDIVRKQYILETQLFYRQLAKVGGVVVYPSMANFALVEIV
                     GGVTADEVMQHLLVRHGVYVRTCSDKIGLDGQFLRIASRGLRENQMIIDALSDVLGSK
                     DS"
     gene            2117..2677
                     /locus_tag="PKY13_01790"
     CDS             2117..2677
                     /locus_tag="PKY13_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78682.1"
                     /translation="MKARLKRAVELLAGAIAWLQVGWVLRTTQPGDKIVVLDIDNTIA
                     DAWPSFLRPFDSQRSRLASLEVLPNVKGVAHDAPVAEGATIIYLSHRNLWEWPVTYRW
                     LRGNGFAVRPDRVLVVPSAAAKVPYLRRLARGRDVTFWDDLSRGHETGEIEFYAEVIE
                     SLAGVSLTYRGWDDIVTLTGKGLERD"
     gene            2967..>4759
                     /locus_tag="PKY13_01795"
     CDS             2967..>4759
                     /locus_tag="PKY13_01795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT78683.1"
                     /translation="MTSILTLGVAIALYPLAIGGRGALMPNLAVPWWVMVLPFALCDG
                     IELRFRIKRSVVFATLVEIPFVIGLFSLSGRDLLLANLIGGVIALTALGYRPSILLFN
                     VSGFALDCGVILVIFHALAPESGTITLSAWFGAALAMMAAAVACSFRVALLGWFRGTR
                     PDRSSLSSGIVMVAVAAINATLTFLALRLVVADARLAWLVGALAVVLVFAYRNYISLL
                     NHHEGLNRLYESTQTLDLVRAQEGSIRGMLDQALDLLGAEVAELTVSLSDGDDARRFR
                     ATRTEGVRALDPDDGGGISPETLALLSPAGDSDGDTDRFGSVESNDQRTLQATLQGSA
                     GPLAVLRVATDRGVTFSEADRRLFEMFVNHASVALQNSQLVDQLRDEVAQREHEALHD
                     ALTALPNRVMFDLQTRRALADRTGSDVVAVALIDIDHFKEVNDTLGHHHGDELLAELA
                     RRLDVLASDRDLVVSRLGGDEFAVLMVTNGGVEAVAKIAADVKAVIEESMEIADVRVD
                     ITASVGISVTDPDGPAVDGSTMLRQADVAMYIAKRQHLGTVFYDRTDDPYSPKRLSLA
                     ADLRHAVERGEIEAYFQPEIDLVTGDVISVEA"
     assembly_gap    4760..4859
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4860..6045)
                     /locus_tag="PKY13_01800"
     CDS             complement(<4860..6045)
                     /locus_tag="PKY13_01800"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT78684.1"
                     /translation="MIDALTTDRSGRVSAQWRTFRAYWLGLTVSTIGDSFTLVALPIA
                     AYTINRSSVLVGFVDMMEILSGVLLGSFLGIVADRSDSRTVMFWSDATRATMLLALSV
                     MAYSDSWNEWTILALAFCLGALRAFHDGAESSMLATIIPPSREMQAFARISSAESISR
                     LIGPFIGGITLEVGLWVAFGVDAMTFVVASIIVLTLPRQRQPASEVHRDDRRSMRAEF
                     AEAVAAMRARPTYLALVGVGAFGNLAGLTMAGLFVPFATDILRLRSVGLGFGLLLAVA
                     GLGGLAANWVANRQASHDPRTAGLSMAVVAAFVLLAGALPSIITSVLAMFAMGGGIAY
                     YQSNYAAYRQDVFAPELLGRVSMVTRTIFYLTMTVGFATGGFIADGLGPDRLFIIFGA
                     IAL"
     gene            6173..7243
                     /locus_tag="PKY13_01805"
     CDS             6173..7243
                     /locus_tag="PKY13_01805"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78685.1"
                     /translation="MSVSSKIKKRVALLAEVARRFPVGGLRRSRSSSADLVFWAHHRY
                     PESWLGYVGAGDLVLKDTALLNALVETGRRFRIVGGPAIGSVTNSTIVYSIDAFNPNR
                     SLNYSASLMTTLRQLEAQGNTLFPSADEAELWENKVVMHRRFDELGIRSPETIVVERD
                     TDVDAAIAAAGFEFPLLVKEPHSNNSQGVHKVGSAAELAALRSKLAGEGAYELLVQRL
                     LDMRRDLRVTMIGGEIVHHYWRINLSDEWMPTTTRKGSGVDFVTFPERWRSTIVGAMD
                     ALGLRNGAFDVCWDGDDLDTEPYFLEVSPAYTPNPPPTAEFADRPYAEFKAQITGAGN
                     YTAAFADLVFEQHRLLVAAWGL"
     gene            7339..>7796
                     /locus_tag="PKY13_01810"
     CDS             7339..>7796
                     /locus_tag="PKY13_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT78686.1"
                     /translation="MITVSGLSKAHGPRTLFRDVTFRLLPGRRIALVGGNGVGKTTLL
                     EIVVGEQDADSGEVHRSKDLRVGYLPQELLVDRSGTVLDEVMSGADHILALGRKLDDL
                     MDAIASTTGPEHDQVLEAYGNAQSQFEQLGGYSLESEARRVLSGLGFSET"
     assembly_gap    7797..7896
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7897..>9007
                     /locus_tag="PKY13_01815"
     CDS             <7897..>9007
                     /locus_tag="PKY13_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT78687.1"
                     /translation="DRANQFIERFRYKATKARQVQSRIKTLEKLEKIQVPDQKAIVAK
                     FGFPEPRRSARTVVEMTDVSVGYDGEPVLTGVNLVVERGDKLSLVGPNGAGKSTLLKL
                     LLGNLEPMSGTCTIGNNVDVAYFAQHQVESLDLSQTVAMEFAARVGTDNQPKGRNLRT
                     VLGSFGFSGEAADRRIGDLSGGERTRLALAVCMANPVNLLVLDEPTNHLDLPSCDVLE
                     DALSAYPGTVLLVSHDRHLIRSVAHDLLEVRNGVVKLHSGVEENVLSPSFSGGTAGPG
                     VKAAPTPPPRQQKTATAVKKEKKAAQAASSQQRKPGGGPAVKELRKNVARIEKALAAA
                     ETEVADLNRQLADPEVFSDHEKAAELGRQFGEAKDR"
     assembly_gap    9008..9107
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9189..9956
                     /gene="rfbF"
                     /locus_tag="PKY13_01820"
     CDS             9189..9956
                     /gene="rfbF"
                     /locus_tag="PKY13_01820"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HOT78688.1"
                     /translation="MKVVILAGGVGSRLSEETDVRPKPMVEIGHRPILWHIMKHYATF
                     GYNDFVLCLGYKGEYIKRYFSESLALSADMTIDFGQGTVESHEVVRDDWRVTLVDTGQ
                     HTETGGRIAQIKKYVGDEPFMLTYGDGVADVDLDELVKFHQNHGNYATVTAVHPTARF
                     GELRMEGDAVTRFDEKPQMHEGWINGGFFVLQPEIFDFIPGDVDWAREPLEGLASAGQ
                     LNAYRHEGFWQCMDTLRDKIYLQGLWDQGAAPWKIWP"
     gene            10069..11091
                     /locus_tag="PKY13_01825"
     CDS             10069..11091
                     /locus_tag="PKY13_01825"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT78689.1"
                     /translation="MKVLLTGADGYIGVRMGHYLLERGHDVTGMDSGFHRVGWLYDAG
                     ERRPETFTKDTRDLTLEDLQGYEAVVHLAEVSNDPVGELNGDVTYKINHEGSLRLAKL
                     AKEAGVERFVHMSSCSVYGAAGERPSKEGDPVGGLTAYAKCKVLIEESVAPLADDNFS
                     PTFMRNATAYGASPRQRFDLVVNDLAASAFLTSEIRMASDGTPWRPFVHILDISHAVA
                     CILDAPRDVIHNEIFNVGNDRSNYQVKEVAEIISDLIPGCTLVFGDSSADKRNYRADF
                     TKIHEQLPGFECAWDVERGAKELLDIFAKIGFTEDLYEFRGHTRIKQIKHLLGTQQID
                     DDFFWL"
     gene            11111..11671
                     /locus_tag="PKY13_01830"
     CDS             11111..11671
                     /locus_tag="PKY13_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOT78690.1"
                     /translation="MKFTELSVAGAYKVELEPNTDDRGIFARAWCATELKDHGLEGHI
                     EQMNISTNVHRGTIRGLHVQAPPHGEAKFFRCIAGRSFHVIVDVRPDSPTYGRWAGVE
                     LAAERFDALYVPPFCAKGYQAQEDGTAVMYGVSSPYTPGAESGLRWDDPAFGIEWPIT
                     DGVIVSDKDKSWPDFTLESGTKGTAS"
     gene            11668..12951
                     /locus_tag="PKY13_01835"
     CDS             11668..12951
                     /locus_tag="PKY13_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78691.1"
                     /translation="MIIVDKALQDRQAAGDPVRVGLVGSGFAGQGFVDQVTGHTPGME
                     ISVVANRTLDGAAQAFRQIGIDDVTVVSTAAELDAAMAAHRPAITDDPTLVTGAERVE
                     AVVEATGEIEFGSHTAVAAIENGKHLVLLNAELDCTLGPILKKKADAAGVVFTDADGD
                     QPGVLMNLAREAEMMGFTPVVYGNIKSLLDHRRTPETQKAFAEGVFQRPKHITSFADG
                     TKIAAEMACVANATGFGVSERGMEGPECKRVEEAVGLFDAEKMYLEGTGIVDYILGAE
                     PSFGVFLLAHSDSWLHERYMKIYKMGEGPIYTFYRPYHLSPLETPLTVARAVLFNDAT
                     ITPKGAPVTDVSAIAKFDLKAGDTLDGVGGFKAYGVMENSPVARAEDLLPMGLTDGCV
                     LKRDLAQDTPITFADVELPAGRLSDQLWREQLAAF"
     gene            12986..14035
                     /locus_tag="PKY13_01840"
     CDS             12986..14035
                     /locus_tag="PKY13_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT78692.1"
                     /translation="MDIEQIRSLTPLEPLPSRVYFVCKALGRWADVLRGDDQTPVALD
                     AEPDRVVTHEDMTILQTYVNLKRAGLAVRLVEQPKVDAFNVVSSLDLQISHHTSQSFI
                     VAFRGDWARPELADLTLTMNGTVATRRTEFPMPHWLQTGLLPRDPARGDRLERLVFKG
                     DMVNLDPRFATDRFRDDLAALGVEFLTQPYVHETKSSGWHDYTDVDAVLAIRAIHPTE
                     LSTKPASKLINAWAAGVPAILGNEPAFRELRRDPLDYVEIATPDEAIAAVLRLKEHPA
                     EYRAMVDHGTLRAADYTNQRVAERWHAFLSGPAETAYRSWLTTPPARREAEFVVRAVR
                     QKISLFTFKRQQSTR"
     gene            14043..15026
                     /locus_tag="PKY13_01845"
     CDS             14043..15026
                     /locus_tag="PKY13_01845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT78693.1"
                     /translation="MSPLVTICLPTFERAKYLRQAIDSALAQTYGNFVLSIGDNSRND
                     ETEQVVKEYDDPRITYRRHPDNLGQQGNWLWLIHNADTPYVASLHDDDVWQPDFLEKT
                     VPVIDADPTVSMVFSDYELIDGDDRLLVDETEALTRQSHRDSLPVGPLDLSLPEALRL
                     VAVWNAPQPAYCAVLRREAVVATEFPDAIDPVYDIWLSYQIARRGERFAFVPGRLTRY
                     RWHPGSSTTTGWSAPEDEIFSRIICENSDAGEVIGEIEAYWASIRWGRAVRMMASPDT
                     RVASRIEFQAASRHLPPAKRVLSALAAQSDTTWSALRLLRVASHKLTGERR"
     gene            15023..15964
                     /locus_tag="PKY13_01850"
     CDS             15023..15964
                     /locus_tag="PKY13_01850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT78694.1"
                     /translation="MTEPIGSSIDLAVVVCTYRRNEPLRQLLERLDEEAARSGELVRL
                     GVAIVDDSPTGEARAVADRYGDRFALGVSYTNTASGNISTARNAAIDGGLALVGADGW
                     LAFIDDDCLPCEGWFDELIAMQRRTGAELVTGPVHDVAPVGAPAWLVEQPFLNMITDY
                     EDGIVPPYGTTANVLIEAAWLRDHPDVRFRHELGKLGGEDMVWFETARAAGIGHRYAL
                     HAPVTEQIPLDRTKFRYQLRNKLWFGNTMYVTNLEAGTSPNRLFLRGAKQFAASLLRP
                     FVRAARREPAQVRYALASAAIGVGQMSGRFGARLDHH"
     gene            16111..17004
                     /locus_tag="PKY13_01855"
     CDS             16111..17004
                     /locus_tag="PKY13_01855"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOT78695.1"
                     /translation="MNDPLPVSVPHHGLERLPADTDPDKVADVLRRDGGLVLEGLVSA
                     AAAATVDAELAPYVEARHAGFDAQFDDVFYGSNTKRIQGLASKSTTFVHEILLNPALL
                     ALADRILLEHCGDYWMSQAETIFIGPGNPVQELHRDDLNWSDAARLGIDLQISVLTAV
                     GDYDAEVGATMVIPGSHDWPLDRPIDTTLARPVELDPGDALVYLGSLVHGGGHNQTED
                     RWRKGVYCSYLLGWLTPEEATARSLTPEVAASLPQRARELLGWSSLRGTTAADGAAAA
                     LKLWQLDDVDLERHEGLFTHK"
     gene            complement(17008..17976)
                     /locus_tag="PKY13_01860"
     CDS             complement(17008..17976)
                     /locus_tag="PKY13_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT78696.1"
                     /translation="MTLTPSPGRASVLDDLAIRHFAALQAVAAEQSFGRAAERLGFTQ
                     SAISQQIAAFEKAIGQPLFDRPGGPRQIELTPVGHVMLRHAQSILGHLDTAAEELAQL
                     LAGEGGRLSVGTFQSITVKVLPEVIGRMRAERPGLEVRLTESNLNEELIDLLIDGAVD
                     LAFIVAPVNDDRLVLTPLGSDPYVVIAPVDDGDAPTMERDELAHRQLIGQIDDDACQR
                     NINQYFWSSEIKPDYAFRTDDNAAVQAMVRVGMGLAVMPYLAIDPNDPSIVVRELSPP
                     MPPRVHFAAQRADRTQAPAADRVIELAREVFQRLEQSQTDTIGIAG"
     gene            18090..18338
                     /locus_tag="PKY13_01865"
     CDS             18090..18338
                     /locus_tag="PKY13_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78697.1"
                     /translation="MNYSNAELNQLAWAIDSDGVNRHEEAAAAVVAQARAAGVVTPLV
                     DVLADTSVPAPVRERAFGKVVNAIVHASATSPEWALAN"
     gene            complement(18411..19517)
                     /locus_tag="PKY13_01870"
     CDS             complement(18411..19517)
                     /locus_tag="PKY13_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78698.1"
                     /translation="MMSLPSTAVITFRDVRHGPWNAHGFALTDLFAEAFGATTLRVAD
                     PATYSQQVLASKGKRLDLVRRLPGRSTVRPQVSTLGPTDLLFVVAHDATDLDQLYVTE
                     PDWLESTATKVLVLIEVWANDARRWPQSYARVLEHFDAVFCTLEEGIGALEEASGVAV
                     QVMAQAVDVLATPFRAEPRLDVLTIGRRHPAQHRLIEAWADEHNGWYHYDTLPAGAVN
                     DVDLHRKVTAMMLSQSAVSICNFARFDDPARIGSTKGTGTRFFETLASGALIAGDLPT
                     DDMFTAEFGGVEGIASLPLACSHLDTDVIAELVAKGRDPRVRCGNRAHALASQDLAHR
                     MRQILGHIGVAEPPLLTERFEALEARRAELLSSV"
     gene            complement(19594..20298)
                     /locus_tag="PKY13_01875"
     CDS             complement(19594..20298)
                     /locus_tag="PKY13_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT78699.1"
                     /translation="MPDATVPPGPPKDRARVRRLADKARYDRATIDAILDAGLIAHVG
                     LIAADGSPVVIPTAYARQGDRVVVHGSAASRMIRTGAGGAAMSLAVTHLDGMVVARSL
                     FESSMAYRSVVVVGYAVPIDDPEEKLDALLHLSDRLLPGRVGEARRPTDKELAATRVF
                     ALPLDEATAKVSDHTVDDAADDLTLPIWAGFIPLELVSGTPVSAPDLAPGVPVPPSVA
                     GWTPTRAAESHRSSES"
     gene            20378..21658
                     /locus_tag="PKY13_01880"
     CDS             20378..21658
                     /locus_tag="PKY13_01880"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT78700.1"
                     /translation="MTAIARPRQSDGEAPTQAASWDPLIRPLALGCFLLALIYNSAAL
                     GGIPGVVLPNSLPLLIGIACVGFALILPRDRAVVVPLGAVGVLAMAILSILWSQDEAS
                     SILWLRSQGAVTLGITAMVIVLPTSNTISVLKAYVRIVLAITMFAVTVEPLARIHIDP
                     LGQAPPLKGWHGYFVHKNIMAAFLVFALATVLAFDKNKVSRYLSLASIAFLFVVSDST
                     TGRMAALVLVAVDIWFLTNQRLSGRSSAALGAATGALVAMVGVAIGASLNAIADAAGK
                     DLTFTGRTKIWSASWNAFLRDPIIGHGVNGLFGTPRTVETASVLREIGFQAGHPHNGL
                     LDVAVQFGAVGIVLVIGVVVSIFRSSVKLQRRSPEVAGWALCAISAIVIMSVGESVFL
                     GSSIATMILLRTVILREATAPQRGVETSSVRRHR"
     gene            complement(21635..24241)
                     /locus_tag="PKY13_01885"
     CDS             complement(21635..24241)
                     /locus_tag="PKY13_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT78701.1"
                     /translation="MRNRTAPTASNRRAVPGRAFTAVAITTLTLLSACSSDDGNSGDA
                     NSPRNERSPQAAPKFTGEAALDLSVASTPAGARMTVAVTAPADATEMQVGVDPSFTTT
                     DWEPVSDEVRLDTDGGYQEVFARFRSGADDADPEVVVAGLDVDLAAKAATSDTPTPTL
                     IGLSSPTTLTVDVEVGRIRRGLEKEGDELVGPDVDAEDLDTGWELDATSGAAPTIADV
                     ERTTRPIDTGHSGDDGQDTSFPVRHRIALTLSEPLTIGTEYELTSPLGDTSTFTIDDR
                     SSRSPAVHANQVGFRPGDVAKQAYLTAPPGDRSFTTSPVFHVVDTADDTSVFDGVATK
                     RETSADGEIGRGDLTGLDVWALDFSAVSAPGRYRVCVERIGCSESLSVDEDSTWLRAA
                     TSIARGLYHQRSGIALGEPTTSIDRPRPDHPDDGLVVHQSTLTSLEAADLTDDKLFEQ
                     LVAGATDEVVDGAWGGHFDAGDWDRRSQHLWMVREVLDLVRVAPDRFASLDLNIPESG
                     DAVPDVLDEAFWTLDLFARLQRADGAVRGGVESARFPDFGTPSWKDDLARYAYAPDAW
                     TTYMYASAAADAAVALRTIDPERSARYADSATRAMTWAEAQDVPSKYADKINYQRAVA
                     AASMLGLTGDATWNQVFLDTTPFAAGPTGVLGCNANEICDSGWNYLWVDRQLTDPAVV
                     ANIEESFRQAATRSADASDSTLFGWCLEDPDVPLVWGLGVGGVPHAMTLLRAYVIDRD
                     PRFLAAAQRCASVTLGGNPLDVSYVTGLGANPARHPLIVDVNTGQLPVWPGTPVYGNH
                     QLGGDTEWVIQYRLDPAGVTGDPYAVPYLQNWYDLADVGPMNEFTVYQSHGPALWVFG
                     VLASASPVSPDR"
     gene            complement(24241..>25748)
                     /locus_tag="PKY13_01890"
     CDS             complement(24241..>25748)
                     /locus_tag="PKY13_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOT78702.1"
                     /translation="RLAEEGWALRADSLERQGRAGAEDAAWWRRLCESAQGLPGFEAW
                     GALHDVSQLIVMTATFTLLGRSLGDAGYGRYAGLYGIVGPIGGLTWSGICLCVLQRRL
                     REGDDISTVARKVTLMSLTLGLVGLAIGTLVGAATIGGTLVVTVTAVMFAELVANGVN
                     QVLFAMVQAEKGFAVATRLRMVVLAIRVTTVLTLFTAGRLSVDPGATAAEARDAASHA
                     IGYLAGGYSIGFLVYLAVLITTILPRHGIPFVLRRPDASTTKLTAQFSLPIAVGVLQQ
                     DGDKTVLNSYGYHAEAGLYAAAFRVVAMGLMPLRALEGAAFQRFLPHNENERNEHTRR
                     AFRYSVLALGISLVIGVGIFICTPLISVILGDDFAKAEAIVPWLLPFLPLTAVSNAPS
                     NGLLGLGKLGVRFGIYAAGAVVSLTLYITMIPLMPAGEPWKGAVIGTIVGEAFLTIAG
                     WVALRHYQQRHNATLDAPPSEPSPDSWADPQPPPPYVGPLPVRQASTPQDR"
BASE COUNT         4113 a         8592 c         8664 g         4079 t
ORIGIN      
        1 ggaagttgaa gcacaggtgc agctcgtccc cgttgccgta gtgggaggcc accagctcgg
       61 tgtcgagcag gtagacctcg ccgacggcca tgcggtcgcc gtcgtaggag tcgagcagga
      121 ttctgagctc gcggagcagt gcgtgggtct cgggccggtc gttgaggatc gtgtggggga
      181 gaccggccac atcgggcggt tcgtcgggga gcgccgggtc cttgccgatc aggtggacca
      241 cgtccatacg gaagccgtcg acgccgcgat cgagccagaa gcgcagcgtg tcgtgcatcg
      301 cggcgacgac ctcggggttg ccccagttga ggtcgggctg ttgggacagg aagcagtgca
      361 ggtagtactt gcccgtcgtc tcgtcgaagg tccatgccgg gccgaaggtc agcgattcga
      421 tccagttgtt gggcgcgccg ccgtccgggg ccgggtcgcg ccagtggtac cagtcgcgtt
      481 tggggttgtc ggtcgaggac cgcgattcga ggaaccaggg gtgctgatcc gacgtgtggt
      541 tggggaccca gtcgatgatg acgcgcagcc cgctaccgtg tgcctcggcc acgagggcgt
      601 cgaaggtggc gagatcgccg aacagcgggt cgactccgca gaagtcggcg acgtcgtagc
      661 cgtagtccgc catcggtgac gggaagaacg gcgacagcca gagggcatcg acgccgaggt
      721 ccgcgatgtg gtcgagatgg cggcggaccc cgtcgaggtc gccgatgccg ttgccggtcg
      781 tgtcgcagaa ggaccggggg tagatctggt acccgacggc ggtcttccac cacgggaggg
      841 ctgggtccga cggttcggtg ctcgacggct cggtgttcga catgggcggc agtctcgcgt
      901 ggcgtcgcgg cgctcgctcg atagcctccc acctcatggg tctacgtgag ttcaccagcg
      961 acgaggcggt cgtcgccgga cggctggccg ctttgaagga cgaggcgggc agccattcac
     1021 cgagcctgct cacgttgcgg gagcagatgc cggagctgga gatcgcggtc gacgcctgct
     1081 tcatctccaa tccctacgcg acacagctgt tcctcgacta cttcaagtcc gagctgctcg
     1141 ataccggcgc catccgcgac gtgctcgagt tctacccgtc gcagaaccag gtcatcggag
     1201 cgctgctggc cggtcacctc ggcatccccg aggaccgtgt cttcgtcggc aacggggcga
     1261 tcgagatcat ccaggcggtg atccacaact tcgccaagcg tcgggtgctc gtgaccctgc
     1321 cgaccttctc gtcgtactac gagttcgccg agggccgggt cgacgtcgtc ttccacacgc
     1381 tcgcgaagac cgacgagttc aagctcgacc cccacgacta cctggccaag gtcgccgaga
     1441 ccgaacccga cgtcgtcacg ctcatcaatc ccaacaaccc cgacggttcc tacttctcct
     1501 acgaggacct gctcacgatc ctgcgcgggc tacccgacga cgtgacggcg atcgtcgacg
     1561 agagcttcat ccacttcgcg ttcgaagatc tcaacttgga catccgttcg atcatcgccg
     1621 agtgcgagca gttcccgaac ctcgtcgtcg tgaagtcgat gtcgaaggac ttcggcattg
     1681 ccggcatccg cgccggctac gccgtgatgc acccggaccg catcgatgcg ctcctgcgca
     1741 acgggtacct gtggaactcg aacggtctgg cggagtactt cttccgtctc tactgccgcg
     1801 ccgacttcct cgagcgctac gacatcgtgc gcaagcagta catcctcgaa acgcagctgt
     1861 tctaccgcca gctcgccaaa gtgggcggtg tcgtcgtgta cccgtcgatg gccaacttcg
     1921 cactggtcga gatcgtcggc ggggtgaccg ccgacgaggt catgcagcac ctgctggtgc
     1981 gccacggcgt ctacgtgcgc acgtgcagcg acaagatcgg tctcgacggc cagttcctgc
     2041 ggatcgcctc ccgcggtctg cgcgagaacc agatgatcat cgacgcgttg tccgacgtgc
     2101 tcggttcgaa ggactcgtga aggctcgcct caaacgggcg gtcgaactgc tcgccggcgc
     2161 gatcgcctgg ctgcaggtgg gctgggtgct tcgcaccacg cagcccggcg acaagatcgt
     2221 ggtgctcgac atcgacaaca ccatcgccga cgcctggccg tcgttcctgc gcccgttcga
     2281 ctcgcagcgg tcccgactcg cgtccctcga ggtgctgccg aacgtgaagg gggtcgccca
     2341 cgacgctccc gtcgccgagg gtgcgacgat catctacctg tcgcatcgca acctgtggga
     2401 gtggccggtc acgtatcgct ggctgcgcgg gaacggcttt gccgtgcgac ccgatcgggt
     2461 gctggtggtc ccgtcggcgg ccgcaaaggt gccgtatctc cggcgactcg ctcggggtcg
     2521 cgacgtcacg ttctgggacg acctctcccg aggccacgag accggcgaga tcgagttcta
     2581 tgcagaagtg atcgaatcgc tcgcgggagt atccctcacc tatcggggct gggacgatat
     2641 cgtcacgctg accggaaaag gcctcgaacg cgactgaggg gcgacccccg caggaatcgc
     2701 ccctcagcgt tgatgactca cttagcgtga cccatgcgcc ttctcccttc aacggagtgt
     2761 ttggtggcat gggggaatct aggtcgaacg gcctaccttt gcaaggggtt cgaggggccg
     2821 ttcgacccag atccgaccgg gcaatgtggg cgtgagcgcc agtgctgggc agaactgttc
     2881 agtaacgcct ggggagaacc gaagagaatc gggtgacccc gacaaccgcg atttcgcgat
     2941 ggactgaggt cgaccggcgc gcggtcgtga cttcgatcct gacgctcggc gtcgcgattg
     3001 cgctctatcc cctggcaatc ggcgggcgcg gcgcgctcat gccgaacctc gcggtgccct
     3061 ggtgggtcat ggtgctcccg ttcgcgctgt gcgacggaat cgaactccga ttcaggatca
     3121 agcgatccgt cgtcttcgca acgctggtcg agatcccctt cgtcatcgga ttgttctcgt
     3181 tgtcgggacg agacctcctt ctcgccaacc tgatcggcgg agtgatcgcg ctcacagcgt
     3241 tgggctaccg cccgtcgatc ctgctgttca acgtctccgg attcgccctc gactgcggtg
     3301 tgatcctcgt gatcttccac gcgctcgcgc ccgagtcggg aaccatcacg ttgtcggcat
     3361 ggttcggcgc agcgctggcg atgatggccg ccgccgttgc ctgttcgttc cgcgtcgcgc
     3421 tgctgggatg gttccggggc acgcgccccg atcgcagctc gttgtcctcg gggatcgtga
     3481 tggtggccgt cgctgcgatc aatgcgacct tgacgttcct cgcgctgcgc ctcgtggtcg
     3541 cggatgctcg cctcgcatgg ctcgtgggtg cactcgccgt cgtcttggtg ttcgcctacc
     3601 gcaactacat ctccctgctg aaccaccacg aggggctgaa ccgcctctac gagtccaccc
     3661 agaccctcga cctcgtgcgg gcccaggaag gctcgatccg cggcatgctc gaccaggcgc
     3721 tcgatctgct cggggccgag gtggcggagc tcacggtgtc gctgtccgac ggtgacgatg
     3781 cgaggcggtt ccgtgccacc cgtaccgagg gcgtgcgggc gctcgacccc gacgacggtg
     3841 gcggcatctc gccggagacg ctcgcgctgt tgtcgcccgc tggtgactcc gacggtgaca
     3901 ccgaccggtt cggcagcgtc gagtccaatg atcagcgaac gctccaggcg acgctgcagg
     3961 ggtcggccgg cccgctcgcc gtccttcgcg ttgcgacgga ccgcggggtc acgttctccg
     4021 aggccgaccg gcgcctcttc gagatgttcg tgaaccatgc aagcgttgcg ctgcagaact
     4081 cccaactcgt cgatcaactc cgtgacgagg tcgcccagcg ggaacacgaa gcgctgcacg
     4141 acgcgctcac cgcgctgccg aaccgtgtga tgttcgacct gcagacccgt cgggccctcg
     4201 ccgaccgcac cggttccgac gtcgtcgcgg tcgcgttgat cgacatcgac cacttcaagg
     4261 aagtgaacga cacgctcggt caccaccacg gcgacgagtt gttggcagaa ctcgcccggc
     4321 ggttggacgt cctcgcttcc gaccgcgatc tcgtggtgtc gcgcctcggt ggtgacgagt
     4381 tcgccgtcct catggtcacc aacggcggcg tcgaggcagt cgcgaagatc gcagccgacg
     4441 tgaaagccgt catcgaggag tcgatggaga tcgccgacgt gcgtgtcgac atcaccgcga
     4501 gcgtcggcat ctccgtcacc gatcccgacg gccctgccgt cgacgggtcg accatgctgc
     4561 ggcaggccga cgtcgcgatg tacatcgcga agcgccagca cctcggcacc gtgttctacg
     4621 accgcaccga tgacccgtac agccccaagc gcctgtcgct cgcggctgac ctccggcacg
     4681 ccgtcgaacg cggcgagatc gaggcgtact tccagccgga gatcgacctg gtcaccggtg
     4741 acgtcatctc ggtcgaggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4861 gagcgcgatc gctccgaaga tgatgaacag ccggtccgga cccaggccgt cggcgatgaa
     4921 cccgccggtc gcgaagccga cggtcatcgt caggtagaag atcgttcggg tcaccatcga
     4981 cacgcgcccg agcagctccg gagcgaagac gtcctggcgg taggccgcgt agttcgactg
     5041 gtagtaggcg atgccacccc ccatcgcgaa catggcgagc accgaggtga tgatcgaggg
     5101 gagcgccccg gcgagcagca cgaaggcggc gacgacggcc atcgacaacc cggcggtgcg
     5161 agggtcatgg ctcgcctggc gattcgcgac ccagttggcg gccagaccgc cgaggccggc
     5221 gacggcgagc aacagaccga acccgaggcc gaccgaccgg agtcgcagga tgtcggtcgc
     5281 gaagggcacg aacaggccgg ccatggtcag gccggccaag ttgccgaacg cccccactcc
     5341 cacgagcgcc aggtaggtgg gtcgggcccg catcgcggcc accgcctcgg cgaactcggc
     5401 ccgcatcgat cgccggtcgt cgcggtggac ctcggaggcg ggttgccgct ggcggggcag
     5461 cgtcaggacg atgatcgagg cgacgacgaa ggtcatcgca tcgaccccga acgccaccca
     5521 cagccccacc tcgagcgtga tgcccccgat gaacgggccg atgagccggg agatggattc
     5581 cgccgagctg atccgggcga acgcctgcat ctcccgggac ggcgggatga tcgtggccaa
     5641 catcgagctc tcggcgccgt cgtggaaggc gcgcaacgcg cccagacaga aggccagcgc
     5701 caggatcgtc cactcgttcc agctgtcgga gtacgccatg acggagaggg cgaggagcat
     5761 ggtggcgcgc gtcgcgtcgg accagaacat cacggtgcgt gaatcggacc ggtcggccac
     5821 gatcccgagg aacgacccga gcagcacgcc ggacaggatc tccatcatgt cgacgaagcc
     5881 gacgagcacc gacgatcggt tgatcgtgta cgccgcgatc ggcagggcga ccagcgtgaa
     5941 cgaatcgccg atcgtcgaga cggtgaggcc cagccagtag gcgcggaacg tccgccactg
     6001 agccgacacg cggccggatc gatcagttgt gagggcgtcg atcatgggga ctgctcgaac
     6061 gtcgggggag gacccctggg aatctagacg ggaacgtcga tcggatcctt gggaggatgc
     6121 gcccggtccg ctcgggcggt tcgcagcgtc cgacccgcta ccgtgagcgc cgatgtcggt
     6181 ctcgtccaag atcaagaagc gggtcgcgct gctcgccgag gtggctcggc ggttccccgt
     6241 cggtggcctc cgccggagtc gctcgtcgtc ggccgacctg gtgttctggg cccaccaccg
     6301 ctaccccgag tcgtggctcg gctacgtggg ggcgggtgac ctggtgctga aggacaccgc
     6361 gctcctcaac gcgctggtcg agaccggtcg gaggttccgc atcgtcggag gccccgcgat
     6421 cgggtcggtc accaactcga cgatcgtgta ctcgatcgac gcgttcaacc cgaaccggtc
     6481 gctcaactac tcggcatcgc tcatgacgac gctgcgccaa ctcgaggcgc agggcaacac
     6541 gctgttcccc tccgccgacg aggccgagct gtgggagaac aaggtggtca tgcaccgccg
     6601 gttcgacgag ctcggcatca ggtcgccgga gacgatcgtg gtcgaacgcg acaccgatgt
     6661 cgacgcggcg atcgctgcgg ccgggttcga gttcccgctg ctcgtgaagg agccgcacag
     6721 caacaactcc cagggtgtcc acaaggtcgg gtccgcggcc gagctcgccg cgctgcggtc
     6781 gaagctggcc ggcgagggcg cctacgagct gctggtgcaa cgcctgctcg acatgcgccg
     6841 cgacctgcgg gtcacgatga tcggcggaga gatcgtccac cactactggc ggatcaacct
     6901 ctccgacgag tggatgccga cgaccacccg caagggcagc ggggtcgact tcgtgacgtt
     6961 ccccgagcgg tggcggtcca cgatcgtcgg agcgatggat gcgctcggtc tccgcaacgg
     7021 ggcgttcgac gtgtgctggg acggggacga tctcgacacc gagccgtact tcctcgaggt
     7081 cagcccggcc tacacgccca atcctccgcc caccgcggag ttcgccgatc gcccgtacgc
     7141 cgagttcaag gcgcagatca ccggtgccgg caactacacc gcggcgttcg ccgacctggt
     7201 gttcgaacag caccgcctcc tcgtcgccgc ctggggcctc tgaacccggg gtggttctgc
     7261 cccgcagttc aatcggcatt ccgatcgatc tgcagggcaa aacggggtgt cgcggcgagc
     7321 cagtaacctg tcacacccgt gatcacggtt tccggactct ccaaggccca tgggccacgc
     7381 acactcttcc gggacgtgac cttccgcctg ctcccgggcc gccgcatcgc gctcgtcggg
     7441 ggcaacggcg tcggcaagac gacgctgctc gagatcgtgg tgggggagca ggatgccgac
     7501 agcggtgagg tccatcgctc gaaggacctt cgtgtcgggt acctccctca agagctgctc
     7561 gtcgaccgca gtgggacggt gctcgacgag gtgatgtcgg gtgccgatca catcctggcc
     7621 ctcggccgga agctcgacga cctgatggac gcgatcgcct cgacgaccgg gcccgaacac
     7681 gaccaggtcc ttgaggccta cggcaacgct cagtcgcagt tcgagcagct cggcggctac
     7741 tccctcgaat cggaggcgcg gcgggtgctc tccggcctcg ggttctccga gaccgannnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgga tcgcgccaac cagttcatcg
     7921 agcggttccg ctacaaggcc accaaggccc ggcaggtgca gagccgcatc aagacgctcg
     7981 agaagctcga gaagatccag gtcccggatc agaaggcgat cgttgccaag ttcggatttc
     8041 ccgagccgcg gcgcagcgcc cgcaccgtgg tcgagatgac cgacgtgagc gtcgggtacg
     8101 acggcgaacc cgtgttgacc ggcgtgaacc tcgtcgtgga acgcggcgac aagctctcac
     8161 tcgtcggtcc gaacggcgcc ggcaagtcga ccctgctcaa gctcctgttg ggcaacctcg
     8221 agccgatgtc gggcacgtgc accatcggta acaacgtcga cgtggcctac ttcgcgcagc
     8281 accaggtcga gtcgctcgac ctgtcgcaga cggtcgctat ggagttcgct gcccgcgtcg
     8341 gcaccgacaa ccagccgaag ggccgcaacc ttcgcacggt gctcggatcg ttcgggttct
     8401 cgggcgaagc cgctgatcga cgcatcggcg acctgagcgg tggggaacgc acccgcctgg
     8461 cgctcgcggt ctgtatggcc aacccggtga acctcctcgt cctcgacgag ccgaccaacc
     8521 acctcgacct gccgagctgc gacgtgctcg aggatgcgct cagcgcgtac cccggcaccg
     8581 tgctgctcgt gtcccacgac cgtcacctga tccgttctgt cgctcacgat ctgctcgagg
     8641 ttcgcaacgg cgtcgtcaag ctgcactcgg gagtggagga gaacgtcctg tcccccagct
     8701 tctctggcgg caccgcaggt cccggggtca aggcggcgcc gaccccgccg ccgcggcaac
     8761 agaagaccgc cacggcggtc aagaaggaga agaaggcggc tcaggcggcg tcgagccagc
     8821 agcgcaagcc gggcggcggc ccggccgtga aggagctccg caagaacgtc gctcgtatcg
     8881 agaaggcgtt ggcggcggcc gagaccgagg ttgccgacct caaccgccag ctggccgatc
     8941 ccgaggtctt cagcgatcac gagaaggcag cggagctcgg ccgacagttc ggggaggcca
     9001 aggaccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct tgcaatagcg
     9121 ggaccgttcc gccaccgtgc gtggcaggaa acgagtgctg cggtgccgca gcgaggagcg
     9181 gttgaacgat gaaggtagtg atcctggcgg gcggggtcgg cagtcggctg agcgaggaga
     9241 cggacgtccg tcccaagccg atggtcgaga tcggtcaccg cccgattctc tggcacatca
     9301 tgaagcacta cgcgacgttc gggtacaacg acttcgtgct ctgcctcggc tacaagggcg
     9361 agtacatcaa gcggtacttc tccgaaagtc tcgctctgtc ggccgacatg acgatcgact
     9421 tcggccaggg aaccgtcgag tcgcacgagg tcgtccgcga cgactggcgg gtcacgctcg
     9481 tcgacaccgg ccagcacacc gagaccggcg ggcgcatcgc ccagatcaag aagtacgtcg
     9541 gcgacgagcc cttcatgctc acgtacggtg acggtgtcgc agacgtcgac ctcgacgaac
     9601 tcgtgaagtt ccaccagaac cacggcaact acgcgacggt caccgcggtg caccccacgg
     9661 ctcggttcgg tgagctgcgg atggagggcg acgccgtcac ccgcttcgac gagaagccgc
     9721 agatgcacga gggctggatc aacggcggct tcttcgtgct gcagccggag atcttcgatt
     9781 tcatccccgg agacgtcgac tgggctcgtg aaccgctcga gggtcttgcg tcggccggcc
     9841 aactgaatgc gtaccgccat gaggggttct ggcagtgcat ggatacgctt cgggacaaga
     9901 tctacctcca ggggctgtgg gaccaaggtg ccgcaccctg gaagatctgg ccgtagccac
     9961 gccctcctga tatccctgta gttgatctga acgccggcgc tgtggcggtc gccggcggct
    10021 gtgcatcgcc gcggcatcat tcctcgtcag ttcgaaaggc aagcagacat gaaggttctc
    10081 ctgaccggtg ccgatggcta catcggcgtt cgtatgggtc actatctcct cgagcgcggt
    10141 cacgacgtga ccggcatgga ctcgggattc caccgggtcg gttggctcta cgacgccggc
    10201 gagcgtcggc cggagacctt caccaaggac acccgcgacc tcaccctcga ggacctccag
    10261 ggctacgagg ccgtggtgca cctcgccgag gtgtcgaacg atcccgtggg tgagctcaac
    10321 ggcgacgtca cctacaagat caaccacgag ggaagccttc gcctcgccaa gctggcgaag
    10381 gaagccggtg tcgagcgatt cgtgcacatg agctcctgct cggtgtacgg cgctgcgggc
    10441 gagcgtccgt cgaaggaagg cgacccggtc ggcggcctca cggcctacgc caagtgcaag
    10501 gtgctcatcg aggagagtgt cgctccgctc gccgacgaca acttctcgcc gaccttcatg
    10561 cgcaacgcga cggcgtacgg ggcgtcgccc cgccagcgtt tcgacctcgt ggtcaatgac
    10621 ctcgcggcaa gcgcgttcct caccagtgag atccgcatgg cgtccgacgg caccccatgg
    10681 cgcccgttcg tgcacatcct cgacatctcc cacgctgtgg cctgcatcct cgatgcgccc
    10741 cgtgacgtga tccacaacga gatcttcaac gtcggcaacg accgttcgaa ctaccaggtg
    10801 aaggaagtgg ccgagatcat cagcgacctg atcccgggct gcacgctggt cttcggcgac
    10861 tcgagcgccg acaagcgcaa ctaccgcgcc gacttcacca agatccacga gcagctcccc
    10921 ggcttcgagt gcgcgtggga cgtcgagcgt ggcgccaagg aactcctcga catcttcgcg
    10981 aagatcgggt tcaccgagga cctgtacgag ttccgcggcc acacccgcat caagcagatc
    11041 aagcatctgc tgggcaccca gcagatcgac gacgacttct tctggctctg agccgggcgg
    11101 tccgatcccc atgaagttca ccgaactctc cgtcgccggc gcctacaagg tcgagctcga
    11161 gcccaacacc gacgaccgtg gcatcttcgc ccgggcgtgg tgcgcgacgg aactgaagga
    11221 ccacggcctc gagggtcaca tcgaacagat gaacatctcg accaacgtgc accgcggcac
    11281 catccgtggc ttgcacgtgc aggcgccgcc gcacggtgag gcgaagttct tccggtgcat
    11341 cgccgggcgg agcttccacg tgatcgtcga cgtccgcccc gactcgccga cctatggccg
    11401 ctgggccggt gtcgaattgg ccgccgagcg cttcgacgcg ttgtacgtac cgccgttctg
    11461 cgccaagggc taccaggcgc aggaggacgg cactgcggtg atgtacgggg tctcgtcgcc
    11521 ctacacaccg ggtgccgaga gcggcctgcg ctgggacgac ccggcgttcg gcatcgaatg
    11581 gccgatcacc gacggggtga tcgtctcgga caaggacaag agctggcccg acttcacgct
    11641 ggagtcggga acgaagggaa ccgcatcgtg atcatcgtcg acaaggcact gcaggatcgt
    11701 caggccgcag gtgaccctgt gcgcgtcgga ctcgtcggat cggggttcgc cggtcagggg
    11761 ttcgtcgatc aggtcacggg acacaccccc gggatggaga tcagcgtcgt cgccaaccgg
    11821 accctcgacg gtgccgcgca ggcgttccgg cagatcggca tcgacgacgt gaccgtcgtg
    11881 tcgaccgccg cggaactcga tgccgccatg gcggcgcacc gcccggcgat caccgacgac
    11941 ccgacgttgg tcaccggtgc cgagcgcgtc gaggcggtcg tcgaggcgac cggtgagatc
    12001 gagttcggca gccacaccgc cgtcgcggcg atcgagaacg gcaagcacct cgtgctgctc
    12061 aacgccgagc tcgactgcac cctcggaccg atcctcaaga agaaggccga tgccgccggc
    12121 gtggtgttca ccgacgccga cggcgaccag cccggtgtgc tgatgaacct cgcccgcgag
    12181 gccgagatga tgggcttcac gccggtcgtc tacggcaaca tcaagtcgtt gctcgaccat
    12241 cgccgcaccc ccgagaccca gaaggccttc gccgaggggg tgttccagcg gccgaagcac
    12301 atcacgagct tcgccgacgg caccaagatc gccgcggaga tggcgtgcgt cgccaacgcc
    12361 accgggttcg gggtcagcga gcgtggcatg gaggggcccg agtgcaagcg ggtcgaggag
    12421 gcggtcggcc tgttcgatgc cgagaagatg tacctcgagg gcaccggcat cgtcgactac
    12481 atcctgggtg ccgagccgag cttcggtgtg ttcctcctcg cccactccga ctcgtggctc
    12541 catgagcgct acatgaagat ctacaagatg ggtgagggcc cgatctacac gttctaccgg
    12601 ccctaccacc tgagtccgct cgagacgccg ctgaccgtgg cccgggccgt gctgttcaac
    12661 gatgcgacga tcacgcccaa gggcgcaccg gtgaccgatg tgtcggccat tgccaagttc
    12721 gacctgaagg ccggcgacac gctcgacggc gtcggcgggt tcaaggccta cggcgtgatg
    12781 gagaactcgc cggttgcccg tgccgaggac ctcctgccga tgggcttgac cgatggttgt
    12841 gtgctcaagc gcgacctcgc ccaggacact ccgatcacct tcgccgacgt cgagctgccg
    12901 gcgggtcgac tgtccgacca gctctggcgg gagcagctgg ccgccttcta ggtctgctgt
    12961 tccactcggt cgctggggag agacgatgga catcgaacag atccgctcac tgactccgct
    13021 ggagccgttg ccctcgcggg tgtacttcgt ctgcaaggcc ttggggcggt gggccgacgt
    13081 actgcggggg gacgaccaga cgccggtcgc gctcgacgcc gagcccgacc gggtcgtcac
    13141 ccacgaggac atgacgatcc tccagacgta cgtgaacctc aagcgcgccg gccttgccgt
    13201 gcggctcgtc gagcagccga aggtcgacgc cttcaacgtc gtgagcagcc tcgacctcca
    13261 gatcagccat cacacgagcc agtcgttcat cgtcgccttc cggggcgact gggctcgtcc
    13321 cgagctcgcc gacctcacgc tgacgatgaa cggcaccgtc gccacccggc ggaccgagtt
    13381 ccccatgccc cactggctgc aaacggggct gctgccgcgt gaccccgctc gcggcgaccg
    13441 cctcgaacgg ctcgtcttca agggcgacat ggtcaacctc gacccccggt tcgcgaccga
    13501 tcgattccgc gacgacctgg cggcgctcgg ggtcgagttc ctcacccagc cctacgtgca
    13561 cgagaccaag tcgagcggct ggcacgacta caccgacgtc gacgcggtcc tcgcgatccg
    13621 ggcgatccac ccgaccgaac tgtcgaccaa gccggccagc aagctgatca acgcctgggc
    13681 ggccggagtc ccggcgatcc tgggcaacga gccggcgttc cgcgaactcc gtcgcgatcc
    13741 actcgactac gtcgagatcg cgacgcccga cgaggcgatc gccgccgttc tgcgcctgaa
    13801 ggagcacccg gccgagtacc gggcgatggt cgaccacggc acgctccgcg ccgccgacta
    13861 caccaaccag cgggtggccg aacgatggca cgcgttcctg tccggtccgg cggagacagc
    13921 ctaccggtcc tggctgacga cgcctccggc ccgtcgtgag gcagagttcg tggtgcgtgc
    13981 cgtccgccag aaaatcagcc tcttcacctt caaacgacag cagtcgaccc gatgagggtt
    14041 ggatgagtcc gctcgtcacc atctgcctcc ccaccttcga acgggccaag tacctccgtc
    14101 aggccatcga ctccgctctg gcgcagacgt acggcaactt cgtgctctcg atcggcgaca
    14161 acagccgcaa tgacgagacc gagcaggtcg tcaaggagta cgacgatccc cgcatcacct
    14221 atcggcgaca ccccgacaac ctcgggcagc agggcaactg gctgtggctc atccacaacg
    14281 ccgacacgcc gtacgtggcg tcgctgcacg acgacgacgt gtggcaaccc gacttcctcg
    14341 agaagacggt ccccgtcatc gacgccgacc ccaccgtgtc gatggtcttc tccgactacg
    14401 agctgatcga cggcgacgac cggctgttgg tcgacgagac cgaggcgctc acccggcagt
    14461 cccaccgaga ttcgttgccg gtcgggccgc tcgatctcag cctgcccgag gcgctgcgcc
    14521 tcgtcgccgt gtggaacgcg ccgcagcccg cgtactgtgc cgtgctccgt cgcgaggcgg
    14581 tggtcgccac cgagttcccc gatgcgatcg accccgtgta cgacatctgg ctctcgtacc
    14641 agatcgcccg ccgtggcgaa cggttcgcgt tcgtgccggg ccgcctcacc cgctaccgct
    14701 ggcacccggg cagctccacg acgacgggct ggtccgcccc tgaggacgag atcttctccc
    14761 gcatcatctg cgagaacagc gatgcgggcg aggtcatcgg cgagatcgag gcctactggg
    14821 cgtcgatccg ttgggggcgc gccgtccgca tgatggcgtc gcccgacacc cgcgtcgcgt
    14881 cgcggatcga gttccaggcg gcctcgcgtc acctgccgcc ggccaagcgg gtgctcagcg
    14941 cgctcgcggc gcagtccgac acgacgtggt cggcgcttcg gctcctccgc gttgccagcc
    15001 acaagctgac gggggaacgg cgttgaccga gccgatcggc agcagcatcg acctggccgt
    15061 cgtcgtctgt acgtaccgtc gcaacgagcc gttgcgacaa ctgctcgagc gcctcgacga
    15121 ggaagcagcg cggtccggag aactggtccg cctcggcgtg gccatcgtcg acgacagccc
    15181 caccggcgag gcacgtgctg tcgccgaccg gtacggcgat cgtttcgcac tgggcgtgag
    15241 ctacaccaac acggcgtcgg gcaacatctc gacggcgcgc aacgctgcga tcgacggcgg
    15301 cctcgcgctc gtgggagcgg acggctggct tgccttcatc gacgacgact gcctgccgtg
    15361 cgagggctgg ttcgacgagc tcatcgcgat gcagcgccgc accggtgcgg agctcgtcac
    15421 cgggcccgtg cacgatgtcg ccccggtggg agcgccggcc tggctcgtcg agcaaccgtt
    15481 cctcaacatg atcaccgact acgaggacgg catcgtgccc ccctacggca ccacggccaa
    15541 cgtcctgatc gaggcggcgt ggctgcgcga ccaccccgac gtccggttcc gccacgaact
    15601 gggcaagctc ggcggtgagg acatggtgtg gttcgagacg gcccgcgccg ccggcatcgg
    15661 ccaccgctac gccctgcatg cgccggtgac cgagcagatc ccgctcgacc gcaccaagtt
    15721 ccgctaccag ctgcgcaaca agctgtggtt cggcaacacg atgtacgtca ccaacctcga
    15781 ggccggcacc tcaccgaacc gcctgttcct gcggggagcg aagcagttcg ccgcatcact
    15841 gctgcggccg ttcgtgcggg ccgcccggcg cgaaccggcg caggttcgat atgccctcgc
    15901 cagcgcggcg atcggcgtcg ggcagatgtc gggccgcttc ggggcccgtc tcgatcatca
    15961 ctgagtgggc cgatccgcaa gcgttccgtt cgttcgttcg cccacctgag agcgctccct
    16021 gatacttcgt cgggctgcta cttctcgcga gcgcggggcc gatacgttga ccagcggcct
    16081 cccccgggct gaacgacaga aggactgctg gtgaacgacc ccctccccgt ttccgtgccc
    16141 caccacggcc tcgaacgcct ccccgccgac accgatcccg acaaggtcgc cgacgtgctc
    16201 cgccgcgacg gcgggctcgt cctcgaaggc ctggtctcgg cggccgcagc ggccaccgtc
    16261 gacgccgagc tcgcccccta cgtcgaggcc cgccacgccg gcttcgatgc gcagttcgac
    16321 gacgtgttct acgggtcgaa caccaagcgc atccagggcc tcgcctcgaa gagcacaacg
    16381 ttcgtgcacg agatcctgct caacccggcc ctgctcgcgc tggccgaccg catcctgctc
    16441 gagcactgcg gcgactactg gatgagccag gccgagacga tcttcatcgg ccccggcaac
    16501 ccggtgcagg agttgcaccg tgacgacctc aactggtccg acgccgctcg gctcgggatc
    16561 gatctgcaga tctcggtgct gaccgcggtc ggcgactacg acgccgaggt cggcgcgacg
    16621 atggtcattc ccggcagcca cgactggccg ctcgaccggc ccatcgacac gacgttggct
    16681 cggcccgtcg aactcgatcc cggtgatgcc ctcgtgtacc tcggttcgct cgtgcacggc
    16741 ggtggtcaca accaaaccga ggaccggtgg cgcaaggggg tgtactgcag ctacctgctc
    16801 ggctggttga cccccgagga ggcgacggct cgttcactca cgcccgaggt cgcggcgtcg
    16861 cttccgcagc gggcacgcga gctgctcggg tggtcgagcc tgcggggcac gacggctgcc
    16921 gatggcgccg ccgctgcgct caagctgtgg cagctcgatg acgtcgacct cgagcgtcac
    16981 gagggcctgt tcacccacaa gtaggcgtca accagcgatg ccgatcgtgt cggtctgtga
    17041 ttgctccagc cgttggaaca cctcgcgggc gagctcgatg acacggtccg ccgccggggc
    17101 ctgggttcgg tcggcgcgct gcgctgcgaa gtggacccgc ggtggcatgg gcggcgagag
    17161 ctcgcgcacc acgatggacg ggtcgttcgg atcgatcgcc aggtacggca tgacggcgag
    17221 ccccatgccg acccgcacca tggcctgcac cgcggcgttg tcgtcggtac ggaacgcata
    17281 gtcgggcttg atctccgagc tccagaagta ctggttgatg ttgcgctggc acgcgtcgtc
    17341 gtcgatctgc ccgatcagtt gtcggtgggc gagctcgtcg cgttccatgg tcggggcgtc
    17401 gccgtcgtcg acgggggcga tcaccacgta ggggtcgctg ccgagcggtg tgagtacgag
    17461 gcggtcgtcg ttgacggggg cgacgatgaa ggcgagatcg acggcaccgt cgatcagcag
    17521 gtcgatcagt tcctcgttca ggttcgactc ggtgaggcgg acctcgaggc ccggacgctc
    17581 ggctcgcatc cgaccgatga cctcggggag caccttcacg gtgatgctct ggaacgtgcc
    17641 caccgagagc cgtccgcctt caccggcgag cagttgggcg agctcttcgg cagcggtgtc
    17701 gagatgcccg aggatcgact gcgcgtggcg cagcatcacg tgaccgacgg gggtgagttc
    17761 gatctgccgc ggcccgcccg gtcggtcgaa caggggctgg ccgatggcct tctcgaaggc
    17821 ggcgatctgt tggctgatgg ccgactgggt gaagccgaga cgttcggctg cccgcccgaa
    17881 ggactgttcg gcagcgaccg cctggagcgc ggcgaagtgg cggatggcga gatcatcgag
    17941 cacggatgcc ctcccggggg atggggtgag tgtcattagc gtatctgatg ataagcatct
    18001 ctgtaaatcg ttgttactta tggaggggcc tccttacact ggaattcaga ccggtacaac
    18061 ccccgccgga acgaagaagg accaccccga tgaactactc caacgctgaa ctcaaccaac
    18121 tcgcctgggc gatcgactcc gacggcgtca accgtcacga ggaagccgcc gcagctgtcg
    18181 ttgcccaggc ccgcgccgcc ggcgtcgtca ccccgctcgt cgatgtcctc gccgacacct
    18241 ccgtgcccgc tccggtgcgt gagcgtgcgt tcgggaaggt cgtgaacgcc atcgttcatg
    18301 ccagcgccac cagccccgag tgggcgttgg cgaactagta ccacttcggg ggcgacctcg
    18361 atctggcccg gcggcccgcc cttgtgtgag ggggcggcgc cgccggccga tcagaccgac
    18421 gacagcagct cggcccgtcg ggcctcgagc gcttcgaatc gctccgtcaa cagcggcggc
    18481 tcggccacgc cgatgtgacc caggatctgg cgcatccggt gggccaggtc ctgggacgcg
    18541 agggcgtgcg cccggttgcc acaacggacg cgagggtcgc gtcccttggc cacgagctcg
    18601 gcaatgacgt cggtgtcgag gtggctgcat gccagcggca gactcgcgat gccctcgacc
    18661 ccaccgaact ccgcggtgaa catgtcgtcg gtgggcagat cgccggcgat gagcgctccc
    18721 gacgccagcg tctcgaagaa ccgggtgccg gtgcccttgg tgctcccgat gcgggccgga
    18781 tcgtcgaagc gggcgaagtt gcagatcgac accgccgact gcgacagcat catcgccgtc
    18841 accttccggt ggaggtcgac gtcgttgacg gcgccggcgg gcagcgtgtc gtagtggtac
    18901 cagccgttgt gctcgtcagc ccacgcctcg atgagtcggt gctgggccgg gtggcggcgc
    18961 ccgatcgtga ggacgtcgag ccgtggctcg gcccggaacg gcgtggcgag cacgtcgacg
    19021 gcctgcgcca tgacctgcac cgcaactccc gacgcctcct cgagcgcgcc gatgccttcc
    19081 tcgagcgtgc agaacacggc gtcgaagtgc tcgaggaccc gggcgtagct ctgcggccag
    19141 cgccgggcgt cgttggccca cacctcgatg aggacgagca ccttggtcgc cgtgctctcg
    19201 agccagtcgg gctcggtcac gtagagctgg tcgagatcgg tggcgtcatg ggcaaccacg
    19261 aacaacaggt cggtcggccc gagggtcgag acctgggggc gaacggtcga ccgccccgga
    19321 agccggcgga ccaggtcgag ccgcttgccc ttgctggcca acacctgctg ggagtaggtc
    19381 gccgggtcgg ccacgcggag tgtggtcgcg ccgaaggcct cggcgaacag gtcggtcagg
    19441 gcgaagccgt gcgcgttcca cggcccgtgg cggacgtcgc ggaaggtgat tactgcagtg
    19501 gaagggaggg acatcatcgt gaccgccgcg ctcagaggga aggacccgtg acgaaaggta
    19561 ccggcacacc ctgaaatcgg tcaacgaaag atgtcaggac tccgatgaac ggtgtgattc
    19621 ggcggcgcgc gtcggcgtcc accccgcgac cgacggcggc accggcaccc ccggagcgag
    19681 gtcgggcgct gacaccggcg tgccactcac cagctcgagc gggatgaagc cggcccagat
    19741 cgggagggtg aggtcgtcgg cagcgtcgtc gacggtgtgg tccgacacct tggcggtcgc
    19801 ctcgtcgagc gggagcgcaa agacgcgggt cgctgccagc tccttgtcgg tgggacgacg
    19861 cgcctcgccg acgcggccgg gcaggagccg atcggagaga tggaggagcg cgtcgagctt
    19921 ctcctcgggg tcgtcgatcg gcacggcgta gcccacaacg acgaccgacc ggtaggccat
    19981 cgacgactcg aacaacgagc gagcgacgac catgccgtcg agatgggtga cggccaagct
    20041 catggcggca ccgccggctc cggttcggat catgcgactc gctgcggagc cgtgcaccac
    20101 cactcggtcg ccctgacgcg cgtatgccgt cgggatgacg accggggagc cgtcggcggc
    20161 aatcaggccg acgtgcgcga tcaggccggc gtcgaggatc gcgtcgatgg tggcgcggtc
    20221 gtagcgggcc ttgtccgcga ggcggcggac gcgggctcgg tctttcggtg ggcccggcgg
    20281 aacggtggca tccggcatgg gcagagtctg ccggaccggc gctcggccgt gtcggtccgt
    20341 tggtcgatcc tccgagctcg tcggtaccat ggtcgaggtg actgccatcg cccgaccgcg
    20401 ccagagcgac ggcgaagcgc cgacccaggc ggcgtcgtgg gatccgttga tccgaccgct
    20461 cgcgctcggg tgctttctcc tcgcgctgat ctacaacagt gcggcgctcg gtggcatccc
    20521 gggagtggta cttcccaact cgctgccgct gctcatcggc atcgcctgcg tcgggttcgc
    20581 cctcatcctc ccgcgcgacc gcgccgtggt cgtgccgctc ggtgcggtgg gcgtgctcgc
    20641 gatggccatc ctgtcgatcc tctggtcgca ggacgaggcg tcgtcgatcc tctggctccg
    20701 ctcacagggc gcggtgacgc tgggtatcac ggcgatggtg atcgtcctgc cgacgagcaa
    20761 cacgatcagc gtcctcaagg cctacgtgcg tatcgtcctg gcgatcacga tgttcgcggt
    20821 caccgtcgag ccgttggccc ggatccacat cgaccccctc ggccaggcgc cgcccctcaa
    20881 gggctggcac ggctacttcg tgcacaagaa catcatggcg gccttcctgg tgttcgctct
    20941 cgccaccgtc ctggccttcg acaagaacaa ggtctcccgg tacctgtcgc tcgcctcgat
    21001 cgcgttcctg ttcgtggtct ccgactcgac cacggggcgc atggccgccc tcgtgctggt
    21061 ggcggtcgac atctggttcc tcaccaatca gcgactgtcg gggcggtcgt cggcagcgct
    21121 cggcgcggcg accggcgcgc tcgtcgcgat ggtcggcgtt gcgatcggcg cctccctcaa
    21181 tgccatcgcc gacgcggcgg gaaaggacct cacgttcacc ggccgcacca agatctggtc
    21241 ggcgagctgg aacgcgttcc tccgcgaccc gatcatcggc cacggtgtca acggcctgtt
    21301 cggcacaccc cgcacggtcg agacggcgtc ggtcttgcgg gagatcggct tccaggccgg
    21361 ccacccgcac aacggcctgc tcgacgtcgc ggtccagttc ggtgccgtgg gcatcgtgct
    21421 cgtgatcggc gtcgtcgtct cgatcttccg gtcgtcggtg aaactgcagc ggcgatcccc
    21481 cgaggttgcg ggctgggccc tctgcgcgat ctccgcgatc gtgatcatgt cggtcggcga
    21541 gtcggtcttc ctcggctcgt cgatcgccac gatgatcctc ctgcgaaccg tcatcctccg
    21601 tgaggcaacc gcaccgcaac gcggtgtgga gacctcatcg gtccggcgac accggtgagg
    21661 ccgacgccag cacgccgaac acccacagcg ccggaccgtg ggactggtag acggtgaact
    21721 cgttcatcgg cccgacgtcg gccaggtcgt accagttctg caggtacggc acggcgtagg
    21781 ggtcaccggt cacgccggcg gggtcgagcc ggtactggat cacccactcg gtgtcgccgc
    21841 cgagctggtg gttgccgtac accggcgtgc ccggccagac cgggagttgc cccgtgttca
    21901 catcgacgat gagcgggtgt cgtgccgggt tggcgccgag ccccgtgacg taggagacgt
    21961 cgagcggatt gccgccgagg gtcaccgatg cgcatcgctg ggcggcggcc aggaatcggg
    22021 gatcgcggtc gatgacgtac gcgcggagga gcgtcatggc gtgaggtaca ccgccgacgc
    22081 cgagccccca caccaacggc acgtcggggt cctcgaggca ccagccgaag agggtcgagt
    22141 cggaggcgtc cgccgaccgg gtggccgcct gccggaagct ctcctcgatg ttggccacca
    22201 cggcggggtc ggtgagctga cggtcgaccc agaggtagtt ccatccggag tcgcagatct
    22261 cgttggcgtt gcagccgagg acaccggtcg gtccggcggc gaacggcgtt gtgtcgagga
    22321 acacctggtt ccaggtggcg tcgcccgtga gccccagcat cgacgctgcg gcgaccgctc
    22381 gctggtagtt gatcttgtcg gcgtacttcg acggcacgtc ctgggcctcg gcccacgtca
    22441 tcgcccgggt cgcggagtcg gcgtaccgcg ccgaacgctc cgggtcgatc gtccggaggg
    22501 cgacggcggc gtcggccgcc gccgaggcgt acatgtaggt cgtccaggcg tcgggcgcgt
    22561 acgcgtaacg cgccaggtcg tccttccacg acggcgtccc gaagtcgggg aaccgggcgc
    22621 tctcgacacc cccacgcacc gcgccgtcgg cccgctggag gcgagcgaac aggtcgagcg
    22681 tccagaacgc ctcgtcgagc acgtcgggga cggcgtcgcc cgactcgggg atgttgaggt
    22741 cgagcgacgc gaatcggtcg ggggcgacac gcacgaggtc gagcacttcg cggaccatcc
    22801 agaggtgttg gctgcgccgg tcccagtcgc cggcatcgaa gtgcccaccc catgcgccgt
    22861 cgacgacctc atcggtcgcg ccggcgacga gctgctcgaa cagcttgtcg tcggtgagat
    22921 ccgcggcctc gagcgaggtc agtgtgctct ggtggacgac gaggccgtcg tcgggatgat
    22981 cggggcgagg tcgatcgatc gacgtcgtcg gctccccgag ggcgatgcca ctccgctggt
    23041 ggtagagacc gcgggcgatg ctcgtcgcgg cccggagcca ggtggagtcc tcgtcgacgg
    23101 agagcgattc cgagcatccg atgcgctcga cacacacccg gtaccggccc ggcgcgctga
    23161 ctgcggagaa gtcgagggcc cacacgtcga ggccggtgag gtcgccgcgg ccgatctcac
    23221 cgtccgcact ggtctcccgc ttggtcgcga ccccgtcgaa cacgctcgtg tcgtcggccg
    23281 tgtcgacgac gtggaacacc ggtgatgtcg tgaacgaccg gtcgccgggc ggcgccgtca
    23341 ggtatgcctg cttcgcgaca tcgccgggcc ggaatccgac ctggttggcg tgcaccgccg
    23401 ggctccgtga cgagcggtcg tcgatcgtga acgtcgacgt gtccccgagg ggcgacgtga
    23461 gctcgtactc ggtgccgatg gtcagcggct cggagagcgt cagcgcgatg cgatgacgaa
    23521 ccgggaacga cgtgtcctgc ccgtcgtccc cgctgtggcc cgtgtcgatc gggcgggtcg
    23581 ttcgttcgac gtcggcgatg gtgggcgcgg cgccggaggt cgcgtcgagc tcccagccgg
    23641 tgtcgagatc ctcggcgtcg acgtcgggcc cgaccagctc gtcgccttcc ttctcgaggc
    23701 cgcgccggat gcgtccgacc tccacgtcga ccgtgagggt cgtggggctg ctcagcccga
    23761 tcagcgtggg tgtcggagtg tcgctcgtcg ctgccttcgc cgcgaggtcg acatcgagac
    23821 cggcgaccac gacctcgggg tcggcgtcgt ccgcgcccga acggaagcgg gcgaagacct
    23881 cctggtagcc accgtcggtg tcgagtcgca cctcgtccga taccggctcc cagtcggtcg
    23941 tggtgaagct cgggtcgaca cccacctgca tctcggtcgc gtcggccgga gcggttacgg
    24001 cgacggtcat ccgtgccccc gccggcgtcg acgccaccga caggtcgagc gccgcctccc
    24061 cggtgaactt cggcgcggcc tggggggagc gttcgttcct cggtgagttg gcgtcaccgg
    24121 agttcccgtc gtccgacgag catgccgaga gcagggtcaa cgtcgtgatg gcgacagcag
    24181 tgaacgcccg gccgggcacc gcccggcggt tcgacgcagt gggggcggtg cggtttcgca
    24241 tcatctgtcc tggggggtgg aggcctggcg gaccggcagc ggcccgacgt acggaggcgg
    24301 cggttgagga tcggcccatg aatcggggga cggctcgctc ggcggtgcat ccagcgtggc
    24361 gttgtggcgc tgctggtagt ggcgcagggc cacccagccg gcgatcgtca ggaacgcctc
    24421 gccgacgatg gtgccgatga cggcgccctt ccacggctcc ccggcgggca tgagggggat
    24481 catcgtgatg tacagcgtga ggctgacgac tgcgccggcg gcatagatcc cgaagcggac
    24541 gccgagcttg ccgagtccga gcagaccgtt ggacggtgcg ttgctcaccg ccgtcagtgg
    24601 gaggaacggg aggagccacg gcacgatcgc ctcggccttg gcgaagtcgt ccccgaggat
    24661 gacgctgatg agcggggtgc agatgaagat gccgacgccg atgacgaggc tgatgccgag
    24721 tgccaggacc gagtagcgga aggcacgccg ggtgtgctcg ttgcgttcgt tctcgttgtg
    24781 ggggaggaac cgctggaacg ccgcaccttc gagcgcccgc agcggcatca agcccatcgc
    24841 gacgacgcgg aacgccgcgg cgtagaggcc ggcctcggcg tggtagccgt agctgttgag
    24901 cacggtcttg tcgccgtcct gctggagcac accgaccgcg atcggcagcg agaactgcgc
    24961 ggtgagcttc gtggtcgacg cgtcgggccg gcgcaagacg aacgggatgc cgtggcgcgg
    25021 gaggatcgtc gtgatcagca ccgcgaggta cacgaggaag ccgatgctgt agccgcccgc
    25081 caggtagccg atcgcgtggc tggcggcgtc gcgcgcctcc gctgcggtcg cccctgggtc
    25141 gacgctgagg cggccggcgg tgaagagcgt gagcacggtc gtgaccctga tggcgaggac
    25201 caccatgcgc agccgggtcg cgaccgcgaa ccccttctcg gcctgcacca tcgcgaagag
    25261 cacctggttc acgccgttgg cgacgagttc ggcgaacatc accgccgtca ccgtcacgac
    25321 gagcgtgcca ccgatcgtgg ccgcgccgac gagggtcccg atcgccagtc cgacgagccc
    25381 gagggtgagc gacatcagcg tgaccttgcg ggcgacggtg gagatgtcgt cgccttcgcg
    25441 gaggcgtcgc tggaggacgc agaggcagat gcccgaccac gtgaggccac cgatcggacc
    25501 gacgatgccg tagagcccgg cgtagcggcc gtacccggcg tcgccgagac tgcgcccgag
    25561 gagggtgaac gtcgccgtca tcacgatcag ctgcgacacg tcgtgcagcg cgccccaggc
    25621 ctcaaagccc ggcaggccct gtgcgctctc gcagagccgg cgccaccagg ccgcatcctc
    25681 ggcgccggcc cggccctggc gctccaagct gtcggcccgc agcgcccagc cctcctcggc
    25741 caggcgga
//
LOCUS       DAOSOA010000012        25736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_1913_length_25736_cov_5.795841, whole genome shotgun sequence.
ACCESSION   DAOSOA010000012 DAOSOA010000000
VERSION     DAOSOA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 25736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25736
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1913_length_25736_cov_5.795841"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKY13_01895"
     CDS             <1..206
                     /locus_tag="PKY13_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78703.1"
                     /translation="EGRVTAPTKLILFGIALVALFLASFTIAGAVVPDDVVTRWTERG
                     ATHDMGTTGTSTTITVHPNGHGS"
     gene            203..2452
                     /locus_tag="PKY13_01900"
     CDS             203..2452
                     /locus_tag="PKY13_01900"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT78704.1"
                     /translation="MTTPITSTTDIELEIGGMTCASCAMRIEKKLNKLDGVTATVNYA
                     TETARVTAATDLDPAVLIAEVEKTGYSAALPAAHGTLPDSDDVVDPELVALRQRLVGA
                     VVLTVPVIVLAMVPALQFEYWQWASLTLAAPVITWAAWPFHRAAWTNLRHGAATMDTL
                     VSVGTLSALLWSLYALFLGTAGVPGMKHGFDLTVTPGDGAGNIYLEVAAGVTMFVLAG
                     RYFEQRSKRRAGAALRALLELGAKDVAVVRDGVETRVPIHDLVVGDEFVVRPGEKIAT
                     DGVVVAGRSAVDASLLTGESLPVEVAEGDAVTGSTVNAGGRITVQATGVGADTQLARM
                     AKLVEDAQAGKAPVQRLADRVSGVFVPIVIAIAVAVLGAWLGAGFPADAAFTAAVAVL
                     IIACPCALGLATPTALLVGTGRGAQLGVLIKGPEVLESTRRVDTIVLDKTGTVTTGRM
                     TLVEVHVEADTERADVLRLAGALEAASEHPIARAVALGAVEDVGALPTPTTFDNLGGR
                     GVVGTVDGHDVVVGSERLLTERSLRLSPALATAKSQAEANGRTAVVAAWDGRAQGVLV
                     VADAVKATSAEAIARFTELGLTTVLLTGDNTAAAEQVGREVGITEVIAEVLPAEKVDV
                     IKRLQSEGKVVAMVGDGVNDAAALAQADLGLAMGTGTDVAIEAADITLVRGDLRSAAD
                     AIRLSRRTLGTIKVNLFWAFAYNVAAIPLAAFGLLNPMLAGAAMAGSSVFVVSNSLRL
                     RSFRSVATP"
     gene            2497..2799
                     /locus_tag="PKY13_01905"
     CDS             2497..2799
                     /locus_tag="PKY13_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOT78705.1"
                     /translation="MDDTTAHHHGYMGDKAKYLARLKRIEGQARGLHRMVDEEQYCID
                     ILTQISAVTKALEGVALALLDDHLRHCVVDAVTSGGPDADAKLKEASDAIARLVRS"
     gene            complement(2815..4221)
                     /locus_tag="PKY13_01910"
     CDS             complement(2815..4221)
                     /locus_tag="PKY13_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT78706.1"
                     /translation="MTRRILVSYLLMTLLILAMLTVPLGLTFAARERDRLFTAIERDA
                     RVLGAESDDAFESSDFSSLPSLVRRYVAETGGRVVMVDAVGRSVVDSSSLSGPSRQFS
                     SRPEIAAALAGRFSSGARTSATLGASIAYVAIPIRHEGEVLGAVRVSYPSTTVDSRTR
                     GVWVQLVSLDAIVLAVVATAGWFVARGLGRPIQRLESAADSLASGDLGARLPVAKGPA
                     DLVRVTTTFNTMAARLQTLVESQRSFLADASHQLRTPLTALRLRIDAVADSGGAPSDD
                     LLAAMAEVDRLAELVDALLSLARLDASAGEIVGVDVGAVVAERVATWLPLAEERDLGL
                     TVDDRSEGAVADVVDGGLEQILDNLIANAADHAPPHSAVVVSVTATGDGVAVAVTDEG
                     PGVTDAHLGRMFDRFWRGPNAVDGGSGLGLPIVLRLAEASGGGVTARLRPEGGLVVEV
                     RLRRSPLGMPVVRRTPGR"
     gene            complement(4218..4880)
                     /locus_tag="PKY13_01915"
     CDS             complement(4218..4880)
                     /locus_tag="PKY13_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT78707.1"
                     /translation="MRVVLIEDDPTISVPLADGLMRSGFDVVAVSTAADGLAAVDGSD
                     LVLLDLGLPDLDGSEVCRRIRQTSSVPIIVISARGDEIDRVLLLESGADDYVVKPFGT
                     RELIARIRAVARRTMPEEAPERQRELGALTIDVAAHRAAMDGVELELTPKEFDLLAYL
                     SSDLGVVHRRRDILESVWDEHWYGPSKTLDVHVAALRRKLGDPAWIETVRGVGYRFGV
                     PG"
     gene            5007..5369
                     /locus_tag="PKY13_01920"
     CDS             5007..5369
                     /locus_tag="PKY13_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78708.1"
                     /translation="MERRTTIGVAAGMTLTIAAIAVAVGLNVAIMSDRSATTGPGTFE
                     PVAETRAPSGSSANPTPAAAAAVETAPAPAPAPTGETTPPTAISTSPAPTSSVHHDDD
                     QREGHDEDHHEDEDGDDD"
     gene            5362..>5661
                     /locus_tag="PKY13_01925"
     CDS             5362..>5661
                     /locus_tag="PKY13_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78709.1"
                     /translation="MTERRRRPHPALGARWVSAGLAVAATVGITSYLEIDSVLHPTAL
                     AAAAAPSATAPPATAPPATAPPATAPPTTIAPATAVPGTGRIVVPPPPPVTVTTIP"
     assembly_gap    5662..5761
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5762..6690
                     /locus_tag="PKY13_01930"
     CDS             <5762..6690
                     /locus_tag="PKY13_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT78710.1"
                     /translation="GLRPADPRPNPGLQRVSVSNTTRRAMGTTAAVTVHGGRLDADDV
                     LAEIDRLEQSWSRFRPDSELSRLNDCGGSLTVVSATLLVLVEHLMAAHRLTGGRFDPT
                     MGAHIAALGYDRTYRDLSDGSRPVRPLPDVVGADDIVVIPSTSAVLLPAGVRLDPGGL
                     GKGLAADLAATMACDAGADGVLVDLGGDIVTRGSAPDGSAWRIDVPSTGVQPRRIFEL
                     HEGAVATSDTSVRTWTVAGVRHRHVLDPHTGLPLERDLRATVVAGAGWWAEAAATAAI
                     ISTAWGDRWVDSLGTIDGISGVDVSEPVAADA"
     gene            6683..7246
                     /locus_tag="PKY13_01935"
     CDS             6683..7246
                     /locus_tag="PKY13_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOT78711.1"
                     /translation="MPDQLWWFLARASGLVTLGASGGSVIWGLLLSTRLIRRRSLPKW
                     LLDLHRFLGALTIAFLVLHVGALVADSFVHFGVADVLVPFHSSWRTVAVGWGVIAAYV
                     LVAVEATSLLRRRLPRRWWARVHLSSFVAFALSVLHAVTAGTDRSNRLFVVLTFAMCA
                     VVVFLTLVRILSASSSPTSQPLPSPNP"
     gene            7338..7829
                     /locus_tag="PKY13_01940"
     CDS             7338..7829
                     /locus_tag="PKY13_01940"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HOT78712.1"
                     /translation="MTETEASAPKKKYFTSWWSKVLLLVGITAVAIQFVPFGVDNPSA
                     RDEPQWDSPTTRQLFMTACADCHSNETKVLWFEHVAPIKWYIADHVKEGREALNVSEW
                     HTNPGRDTDEFAEVVEEGSMPPDYYTYFGLHSDSKLTETEKQQLIDGIEKTLAADPPT
                     SGR"
     gene            complement(7849..8103)
                     /locus_tag="PKY13_01945"
     CDS             complement(7849..8103)
                     /locus_tag="PKY13_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78713.1"
                     /translation="MVGAAGPMLVGGLGSAVVVAGVLALRTILRPDAGSAPPSDEPTV
                     ARFSDPALGSLFGELRLLVSVVAVGAGLVGAALVWGISAA"
     gene            8347..9027
                     /locus_tag="PKY13_01950"
     CDS             8347..9027
                     /locus_tag="PKY13_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT78714.1"
                     /translation="MTDELLIVEDDPGIRSGLVRALTNAGYACRPAPDAAAARAVIDP
                     PGLVLLDLGLPDADGLDLARELHDRWPDVPIVMVTARAEEMDVVVGLDSGAIDYVTKP
                     FRLAELLARIRAHLRRPAPAADAPALVGAGVIVDLGARIVRLDGEEISLRAKEFDLLV
                     ALMSNQGNVVTREELMAQVWDEHWFGSTKTLDVHISSLRRRLGEGPGEPSRITALRGV
                     GYRWERDA"
     gene            9046..>10090
                     /locus_tag="PKY13_01955"
     CDS             9046..>10090
                     /locus_tag="PKY13_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT78715.1"
                     /translation="MRRRILTAIVGVTALATIVLTLPLVFLMASREHNDSDRELTLVA
                     EQVANELTSDSDFSASGLELPPIDDALITVGVYLPDGELVAGDGPTPADELTRSAGLL
                     SSLDNVDDHRVLVRPIVEREQKVAVIRVAEPLSEAERKIWRDILVLVGIDAAALIVAA
                     VVGSWVTSRLVRPLRAIRDDAVRLGEGDFSIVPTPSGIDELDATAGALADTAARLDGV
                     LQRERSFTADASHQLRTPLTSLRLVIETELQSPGADPTPVLDEALGEIDRLERTITTL
                     LRVARDQPITRAPLDIDQWLGTVVGRWQAVFDERGRSITGTTGVGGSPRVSLSVLDQI
                     ADILLQNAVQHGRG"
     assembly_gap    10091..10190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10191..11075)
                     /locus_tag="PKY13_01960"
     CDS             complement(<10191..11075)
                     /locus_tag="PKY13_01960"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT78716.1"
                     /translation="MRTPNDLIELRGTMPDLDRRSGLFPALLKHWRRRRGMSQLDLAL
                     IADVSARHVSFLETGRSRPSPEMVVQLALALALPLRQVNAMLRAAGHDPIYDESDDVL
                     PAVVTDALTLLKAHHEPFPLVVVDRSYRVLDLNEGALGLFGVLLGSGAAEPSGEFNDV
                     IGAGVNLARLTFDPLGAQPWIANFAEVGRDLLWRIQREALADPGDDELSRLLDDLLAL
                     PTIDPDWRQPDLQRPSDPALVLHLRGQGLDLRFLTTITAFQAPQNVAVENLRIETWFP
                     FDEATAAACRQLGPKPPPA"
     gene            11196..12092
                     /locus_tag="PKY13_01965"
     CDS             11196..12092
                     /locus_tag="PKY13_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT78717.1"
                     /translation="MSTLTPAEATDLFRAAPHRYLDVGAGQAAYRRVGTGPDVLFVHG
                     WPVSGATFRRLLPHLADHVTCHVIDLPSSGSSRFDDTTPLSIEQHITTVRRVVDLLEL
                     PSFAAVGHDSGGLIARHAFAGDPRLRALGLVNTEQPQGLSRRFRLFLAGRRLPGFGAA
                     LGWLAGRPKLRRNGFVLGDAFADRSLLDGEFDEFFLRPLHEIPSRRRAAIRVLRTFDE
                     RYVLELGALHRRIDVPVQLVWGDQDPFFPVAWAEEMAGTFPDARLTVVEGAGLFSHEE
                     RPTEVAAALLPVLTGHPHDAVG"
     gene            12226..12669
                     /locus_tag="PKY13_01970"
     CDS             12226..12669
                     /locus_tag="PKY13_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOT78718.1"
                     /translation="MTTQHIQIAIKATPEAVWTALTDGAITPAYYVGFEANFDLTPGA
                     AYSYTAGGGDMITGVVTAVEPASTLTTTFNGHWDPAVADLPESTVTFTLGAPAMPLPG
                     ITVLSCRHEGLDDSPAAAALESGWVTILSGLKTLLETGRPMVDVA"
     gene            12708..13358
                     /locus_tag="PKY13_01975"
     CDS             12708..13358
                     /locus_tag="PKY13_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT78719.1"
                     /translation="MKSYQQYCGLARGLDLVGDRWTLLVIRELALGDRRYAQLQRDLP
                     GIPTNLLADRLRSLTASGLIEPTAGTARSGGYALTPRGREILPVLHALVRWSTPTMLD
                     GPDADDHQSARWVAFAAMAYLRSAPAGSPVSIKLSCGAEHVVIRADHDVTISLDDELP
                     TDVTIDGNAWALMGIVSGALPIGRVRAVDDSAAIVGTQASRRRATALIAHNTAAAS"
     gene            13355..13489
                     /locus_tag="PKY13_01980"
     CDS             13355..13489
                     /locus_tag="PKY13_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78720.1"
                     /translation="MNAGAGEPGVGDRYRDARLRITALLARSLTGGIPDDVFVDLDEG
                     "
     gene            complement(13628..14218)
                     /locus_tag="PKY13_01985"
     CDS             complement(13628..14218)
                     /locus_tag="PKY13_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOT78721.1"
                     /translation="MRISRALAVLTLPVALLAAGCSDDSDTADDTTTTEAPATTEAPD
                     TTEAPATTEAPEASGTIVDIAAGNPDFSTLVDLVGKAGLAEALSGEGPFTVFAPTNEA
                     FAKVDAATLEALAADPTGALADVLKLHVVSGKIMAADAVAAAGTSIETLGGGKLKVEV
                     NGGEVTVGGAKVVTTDIVASNGVIHVLDSVITAANG"
     gene            complement(14302..14982)
                     /locus_tag="PKY13_01990"
     CDS             complement(14302..14982)
                     /locus_tag="PKY13_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78722.1"
                     /translation="MTDHPALHLDDVTKTYQVGDDEIVALADIDLTVGSDEIVALIGP
                     SGSGKTTLCSIAGGILAPTSGEVTVAGTSISGYSDRQLTEFRQSNIGFVFQSVNLIPF
                     LTARENLLVVDELGRRTGQAARDRADTLLEELGLTDRRNNLPEQLSGGQRQRVAIGRA
                     LMNEPTLVLFDEPTSALDSKLGEQVMELIQNEMKSRGTAAIIVTHDDRITHYADRTVH
                     IRDGRLDG"
     gene            complement(14979..16076)
                     /locus_tag="PKY13_01995"
     CDS             complement(14979..16076)
                     /locus_tag="PKY13_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT78723.1"
                     /translation="MKLAWRELRRRPGRFAAATAILFLLGSLLLLLSGLVEGLNANND
                     GLLRSQSADLIVFSATSEESIPQSLVPADTVAAIAATDGVAATGSLGLVQLGGRLPDA
                     APRDLIDVVVVGYDRPFAGLPDSMPDGDAWVDSTLRDRGVRVGTVIEVGAARTPLRVA
                     GFADDVGFQTQPTMLVSSASLREVVAANKPDAVLPEGATQVVVVDTSTPAATARAIDE
                     ATGSTSTVTRAAAADAIPDTGGGVLQQIIGLTVVIAAAVVALFFALLTGERVGLYGVL
                     KAIGARNRSIFAAVITQAVVLSLIAGVIASVVAIVFEAALPADSIPFTLTPARVGTSL
                     ALLLLASVLGASFSLRRVLRIDPASAIGTST"
     gene            complement(16073..17194)
                     /locus_tag="PKY13_02000"
     CDS             complement(16073..17194)
                     /locus_tag="PKY13_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78724.1"
                     /translation="MFLALREIRRSKVRFGLLIAAVALLAFLILFQSAIQNSLIRQFV
                     GGVRHQTAPVLVFNVDGRRFPQSSSVSPELEEKVRATEGIGGIAAIYQGTFPIEAGGD
                     TQATSVLGYADPDLGGPGPLLEGRLPEAPGEVVANAGNEEDGFGIGSTVTVQPAGIDL
                     EVVGLADDVGINVLPTVFTTADTYLTVLQTRNPAATLDVPNVLGVSPASGTTVAELTT
                     RLNRADESIDALSRVDAAELNPGIESIKQSFTVILVLFGLVVPLVCGLFFLILTFQKA
                     SSLVLLRALGAPARRLVEALLVQVSLVLGVGLGIAVGGFALLTRGKTGSLRLRFEPTN
                     VGFWVTTLVVLGVVSAVASIRRVLAIDPNQATGGGGAGR"
     gene            complement(17447..17914)
                     /locus_tag="PKY13_02005"
     CDS             complement(17447..17914)
                     /locus_tag="PKY13_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HSP20 family small heat-shock protein"
                     /protein_id="HOT78725.1"
                     /translation="MLMRTDPFRDLDRLTQQLLGTAARPAAMAMDAWRDGDEFVIELD
                     IPGVSADSIDIDVERNVLTVRAERPQPEGHRDYVAAERPRGVFSRQIVLGDTLDTDRV
                     RAGYDAGVLVLRVPISERAKPRKISIATAPVEPSALDASSAERMSIEQPTPAA"
     gene            18040..18333
                     /locus_tag="PKY13_02010"
     CDS             18040..18333
                     /locus_tag="PKY13_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT78726.1"
                     /translation="MANGEDDPTRGLYGISVAAELVGNGEQNIRLYERRGLVTPARSA
                     GGTRRYSDHDLGRLRRIAELLADGLNLAGVGRVLELEDEIGRLRAERDAGDRR"
     gene            complement(18345..18776)
                     /locus_tag="PKY13_02015"
     CDS             complement(18345..18776)
                     /locus_tag="PKY13_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78727.1"
                     /translation="MPTDRSDVPSTDAPTHIVRLVGVYDADGSLRGELTYWVGARLGR
                     AHCSLCDITHGLVRERSEWRTCRDGLPVPFDAYHRDDQPPEVRAATGGVAPGVVAETT
                     DGIVHLLMPADLDACAGSVDRLVDALDEAIRREGLHWPDRT"
     gene            complement(18781..19518)
                     /locus_tag="PKY13_02020"
     CDS             complement(18781..19518)
                     /locus_tag="PKY13_02020"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HOT78728.1"
                     /translation="MTQESTHHPSGPLLLAVALAAVAGFVDAHIYLAVAPIFVANMSG
                     NLVRIGMLTGVRNWGQAVGAAAAVVAFAAGVVVSIVEHDRRLRRWGHLRPEVLLAVES
                     VLLVAVAVLRSRHGVGFHPRLTGSAIPIVVVAAAAMGVQAATLRRVGDVAVSTTYGTG
                     AIVRIGEKVALAGRRADRTSAHRRRVTVLVLATVLVSYVAGAAVAAALGSSKWLLFLP
                     AGVLVVSTALVATRSRRAGARDRGAVP"
     gene            complement(19804..21078)
                     /locus_tag="PKY13_02025"
     CDS             complement(19804..21078)
                     /locus_tag="PKY13_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT78729.1"
                     /translation="MSDSPLDFASTDLTDPTPYDDPWAFYAWLRAEHPVWFDERSGLY
                     AVSRHADVVTASRDSTYFSSAAGVRPVNLVPLSIVSMDDPEHARQRRVLSKGFTPRQV
                     RSMTDHVRELTNQVIDEVEASGEIDFVSDLAMHVPLIVISELLGIDPGMRNALYDWSD
                     AMIGGEATAGAPGPATDRAAIAFVEYTTLIGELIAERRTDPKGDLISVLTAAYDSGEL
                     AYDDATKARYEDAQAGAEGADVSDLTNDELMMFCVLLMVAGNETTRNAIAGGLRAFSQ
                     FPGERDRLVQDPSLIDGAVEEILRWTSPVLNFTRTVTEPVALGGVELSAGDQVLLLYQ
                     SANRDESVFEDPDTFRIDRSPNPHIAFGFGSHFCMGANLARMEIKVVFEELFRRLPDI
                     AATDPTGPVDRSASAFVAGIHRLPARFTPVGS"
     gene            complement(21081..21644)
                     /locus_tag="PKY13_02030"
     CDS             complement(21081..21644)
                     /locus_tag="PKY13_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT78730.1"
                     /translation="MPAFPREELEAMVEKWLDVNRQAEASGDWRVMAECYAEDATYGW
                     NYGPEVNFMAVGRDQIRDWAIGLEMDGLDGWTYPYETVVIDEKIGQVIGLWRQVADAK
                     RPDGSSYEIAGIGGSWFKYAGNMEWAWQRDWFDLGNAGSLFMEMMSADVLSEGMTSRM
                     HRALGKQPGHYPVNQTAAPLWPEDRKD"
     gene            complement(21821..22864)
                     /locus_tag="PKY13_02035"
     CDS             complement(21821..22864)
                     /locus_tag="PKY13_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOT78731.1"
                     /translation="MKAIVYHGPGHKAWEDVPDPVVIDDTDAIIRVDAVTICGTDLHI
                     LKGDVPAVTDGRILGHEAVGTVETVGAGVKNVKVGDRVLVSCITACGACQYCREGSYS
                     QCLGGGGWILGHKIDGTQAEKVRVPFADTSTYPIPDGVTDEQILMLADILPTGYEVGV
                     LNGQVRPGDVVAVVGAGPIGLSAIMGSKLFSPSRIIAIDLADTRLDAAKQFGADVVIN
                     NSHTDAVAEVMAMTDGLGADVAIEAVGVPATFELATRLARPGGHIANIGVHGEPATLH
                     LEDLWIRNVTITTGLVDTSSTPTLLRLAQGHQMEVDRFITHHFTLDQFDEAYDVFSRA
                     ADTGALKVVLTRS"
     gene            complement(22978..24435)
                     /locus_tag="PKY13_02040"
     CDS             complement(22978..24435)
                     /locus_tag="PKY13_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOT78732.1"
                     /translation="MRRMIGTGLGLLLSASIVASCGPDSPAVDTGAEQLVDGYANVLV
                     TTGDGAKLLSVEPRVPIANGPGAAAVVVDIDRSARYQSMAGVGAAMTDSSAWVLSTRL
                     NDVTRTQVINDLFGRRGANLSVVRVPLSSTDFSLDDFTYDDIELPGEDPDLTAFSLAR
                     EEQQRFPLLRAALAVNPDLALIGSAWTAPGWMKSGASADRLGVVGGTLADGMTEVYSR
                     YLRRVVDGYADAGVPLALLTVQNEPAHETDNYAGMVLSPEQEADLAIELADGLQTDGS
                     STELLVHDHNWNGAPRALDVLARVAGTPRIAGSAFHCYSGNPSAQSTVHNAYPDLDIH
                     FTECTGTESSGTFESNLLWNARVLLVDSVRNWSRSVVLWNLALDERSGPRVGGCRNCR
                     GVLTIHPDGHVTRNEEFYALAQFGRGVDVGATRIGSSSATGGVHNVVFENPDGSTVAF
                     LVSTASQPVDVAVTDGDRSVAVTLPARSVVTLRWA"
     gene            24598..24936
                     /locus_tag="PKY13_02045"
     CDS             24598..24936
                     /locus_tag="PKY13_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78733.1"
                     /translation="MGSMTRGGRWRSGRLLVVALLAVWGVGALVGGALPAGAPADQAA
                     GSELESVVGHRAAVTVLAARAPRSGDELASESVDDAPSADRAVPTVGFAATPLRATGP
                     APGRAPPNGR"
     gene            24994..>25736
                     /locus_tag="PKY13_02050"
     CDS             24994..>25736
                     /locus_tag="PKY13_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT78734.1"
                     /translation="MTSPRRYALLALLPLTALLAACGDDTSTADDAPDGTAGDVSGQT
                     ITLYSGRDEELIAPLIEKFTDDTGIEVDVRYGNSAEMGAQLLEEGEDTPADVFYSQEV
                     GAVGVLAEAGLLASLPNELVGTVDERFRPDGDNEWVGVTGRSRVIVYNPNLTDAPVGV
                     EELTDPKYRGQVAIVPSNAGFQAFITGFRASQGEDAARTWLEDLIANEPITDIASNGD
                     VLEAVEAGDVAIGLINHYYWARDERQPNLT"
BASE COUNT         3905 a         9032 c         8889 g         3710 t
ORIGIN      
        1 gtgagggtcg ggtgaccgct ccgacgaagc tgatcctctt cggcatcgcg ctcgtcgcgt
       61 tgttcctcgc atcgttcacg atcgccggtg cggtggtccc cgacgacgtg gtgacccgct
      121 ggaccgaacg cggcgccacc cacgacatgg gtaccaccgg caccagtacc acgatcaccg
      181 tgcacccgaa cggacacgga tcgtgaccac gcccatcacc tccaccaccg acatcgagct
      241 cgagatcggc ggcatgacct gcgcgtcgtg cgccatgcgc atcgagaaga agctcaacaa
      301 gctcgacggc gtcacggcga cggtcaacta cgccaccgag acggcgcggg tcacggccgc
      361 caccgacctc gatcccgcgg tactgatcgc cgaggtcgag aagaccggat acagcgcggc
      421 cctgcccgcg gcgcacggaa ccctccccga tagcgacgac gtcgtcgacc ccgagctggt
      481 cgcgctgcgg cagcgactcg tcggcgcagt ggtgttgacc gttcccgtga tcgtcctggc
      541 gatggtcccc gccctgcagt tcgagtactg gcagtgggcg tcgctcacac tcgccgcgcc
      601 ggtgatcacc tgggcggcct ggcccttcca ccgcgccgcc tggaccaacc tccgccacgg
      661 cgccgcaacc atggacacgc tggtgtcggt cggcacgctg tcggcactgc tctggtcgtt
      721 gtacgccctg ttcctcggca ccgccggggt gccgggcatg aagcacggct tcgacctgac
      781 cgtcaccccc ggtgacggcg ccggcaacat ctacctcgaa gtcgccgccg gcgtcaccat
      841 gttcgtcctg gccggtcgct acttcgagca gcgatccaag cgccgcgccg gcgccgcact
      901 gcgggcgctg ctcgagctgg gagcaaagga cgtcgccgtc gtgcgcgacg gcgtcgagac
      961 ccgggtcccg atccacgacc tcgtcgtcgg cgacgagttc gtcgtccgcc ccggcgagaa
     1021 gatcgcaacc gacggcgtgg tcgtggccgg ccgatctgcc gtcgacgcga gcttgctgac
     1081 cggcgaatcc ctccccgtcg aggtggccga gggcgacgcc gtgaccggtt cgacggtgaa
     1141 cgcgggtggc cgcatcaccg tgcaggcgac cggcgtcggc gccgacaccc agttggcgcg
     1201 gatggcgaag ctcgtggagg acgcccaagc cggcaaggca ccggtgcaac gcctcgccga
     1261 ccgtgtgtct ggcgtcttcg tgccgatcgt catcgcgatc gcggtggccg tcctcggcgc
     1321 ctggctgggc gccggattcc ccgccgacgc ggccttcacc gccgccgtcg ccgtgctcat
     1381 catcgcctgt ccgtgtgcgc tgggcctggc gacgccgacg gcgctgctcg tcggcacggg
     1441 gcggggcgcg cagctcggtg tcctgatcaa gggcccggag gtgctggagt cgacccgccg
     1501 tgtcgacacg atcgtgctcg acaagacggg cacggtgacc acggggcgca tgacgctcgt
     1561 cgaggtgcac gtcgaggcgg ataccgagcg cgctgacgtg ctgcggctcg ccggggcgtt
     1621 ggaggcggcg tcggagcatc cgatcgcccg ggccgtcgcg ctcggggccg tcgaggacgt
     1681 cggcgccctc cccacgccga ccacgttcga caacctcggc ggccgcgggg tcgtgggcac
     1741 cgtcgacggc cacgacgtgg tggtcgggag cgaacgattg ctgacggagc ggtccctgcg
     1801 cctctccccc gcgctcgcca cggccaagtc gcaggccgag gcgaacgggc gcaccgcggt
     1861 cgtcgccgcc tgggacgggc gggcgcaggg tgtgctcgtc gtagcggacg ccgtcaaggc
     1921 gacgagtgcc gaggcgatcg cccggttcac cgagctgggg ctgaccaccg tgctcctcac
     1981 cggcgacaac accgctgccg ccgagcaggt cggccgtgag gtcggcatca ccgaggtgat
     2041 cgccgaggtc ctgcccgccg agaaggtcga cgtgatcaag cggctgcagt ccgagggcaa
     2101 ggtggtcgcc atggtcggcg acggcgtgaa cgacgccgcc gccctcgccc aggccgacct
     2161 cggtctcgcg atgggcaccg gcaccgacgt cgccatcgaa gccgccgaca tcaccctcgt
     2221 ccggggcgac ctccgctcgg cggccgatgc catccgtctc tcccgtcgga ccctcggcac
     2281 catcaaggtc aacctcttct gggcgttcgc ctacaacgtc gccgccatcc cgctggcggc
     2341 gttcggcctc ctcaacccga tgctcgccgg cgctgcgatg gccggctcca gcgtgttcgt
     2401 cgtcagcaac agcctgcgtc tgcggtcctt ccgttccgtc gcgacaccct gaccccgttc
     2461 cgtcccccgg tccccctccc gaaaggcacc cgtcgtatgg acgacaccac cgcacaccac
     2521 cacggctaca tgggcgacaa ggcgaagtac ctcgcccgcc tcaagcgcat cgagggccag
     2581 gctcgcgggc tccaccggat ggtcgacgag gagcagtact gcatcgacat cctcactcag
     2641 atctccgcgg tgaccaaggc gttggaaggc gttgcgctcg ccttgctcga cgatcacctg
     2701 cggcactgcg tcgtcgacgc cgtgaccagc ggtggccccg acgccgacgc caagctcaag
     2761 gaggcgtccg acgccatcgc ccggctcgtg cgctcctgag ggccggggct cccctcaccg
     2821 acccggcgtg cgacggacga ccggcatgcc gagcggggaa cgccgaaggc ggacctccac
     2881 cacgagcccg ccctccgggc gcagccgggc cgtgactccg ccgcccgacg cctcggcgag
     2941 acgcagcacg atggggagtc cgagcccgct cccaccgtcg acggcgttgg gcccgcgcca
     3001 gaaccggtcg aacatgcgcc ccagatgagc gtcggtcacg cccgggccct cgtcggtgac
     3061 ggcgaccgct acgccgtcgc cggtcgccgt caccgagacg accactgcgc tgtggggtgg
     3121 cgcatgatcg gcggcgttgg cgatgaggtt gtcgaggatc tgctccaggc ccccgtcgac
     3181 gacgtcggcg accgcgccct cggaccggtc gtcgacggtg agcccgaggt cgcgctcctc
     3241 ggccaacggc agccaggtcg cgacccgttc cgcaaccacg gcgccgacgt cgaccccgac
     3301 gatctccccc gccgacgcgt cgagccgcgc caacgacagg agcgcgtcga ccagctcggc
     3361 gagacggtcg acctcggcca tcgcggcgag caggtcatcc gacggtgccc cgccggagtc
     3421 ggcgacggcg tcgatgcgga ggcggagggc cgtcagtggc gttcggagtt gatgggacgc
     3481 gtcggcgagg aaggaccgct gcgactcgac cagcgtctgg agccgcgccg ccatggtgtt
     3541 gaacgtggtc gtgacccgca cgaggtcggc aggccccttc gcgaccggca gccgggcacc
     3601 gaggtcgccc gaagcgagcg aatcggccgc cgattccagc cgctggatgg gccgaccgag
     3661 acctcgggcg acgaaccagc ccgccgtcgc gacgacggcg agcacgatcg cgtcgaggga
     3721 gaccagttgg acccacaccc cccgggtgcg gctgtcgacc gtcgtcgacg gatagctgac
     3781 ccggacggcc ccgaggacct cgccctcgtg gcggatcggg atcgcgacgt acgcgatcga
     3841 ggcgccgagc gtggccgacg tgcgagcccc gctcgaaaag cgaccggcga gcgcggctgc
     3901 gatctcgggc cgactcgaga actggcgtga cggtcccgac aacgagctcg agtcgacgac
     3961 cgaccgaccg acggcgtcga ccatcacgac gcgcccaccg gtctcggcca cgtaccggcg
     4021 aaccagggac gggagcgagc tgaaatcgga gctctcgaac gcgtcgtcgg actcggcacc
     4081 gagcacgcgt gcgtcgcgct cgatggcggt gaacaggcgg tcgcgctcac gcgccgcgaa
     4141 ggtcagcccc agcggaaccg tgagcatcgc gaggatcagc agggtcatca gcaggtagct
     4201 gacgaggatc cggcgggtca tcccgggacg ccgaaccggt agcccacccc acgcaccgtc
     4261 tcgatccaag ccggatcgcc gagcttgcgg cgaagggcag caacgtggac gtcgagggtc
     4321 ttgctgggcc cgtaccagtg ctcgtcccag accgactcga ggatgtcacg tcgacggtga
     4381 acgacaccga ggtcggacga caggtaggcg agcaggtcga actccttggg cgtgagttcg
     4441 agttcgaccc cgtccatggc ggcgcggtgc gccgccacgt cgatggtcag cgcgccgagc
     4501 tcgcgttgcc gctccggcgc ctcctcgggc atggttcgac gggcgacggc acggatccgg
     4561 gcgatcagct ctcgggtacc gaagggcttc acgacgtagt cgtcggcgcc cgattcgagg
     4621 agcagcacac gatcgatctc gtcgccgcgg gcgctgatca cgatgatcgg cacggaggag
     4681 gtctgccgga tgcggcgaca gacctccgaa ccgtcgagat cgggaaggcc gaggtcgagg
     4741 agcacgaggt cgctgccatc gacggcagcg aggccgtcgg cggcggtcga gacggcgacc
     4801 acgtcgaagc cggaccgcat caggccgtcg gcgaggggca ccgagatcgt cgggtcgtct
     4861 tcgatcagca ccactcgcat gccaccacca tggcggacgg gacggggcgt ccggtgggtt
     4921 ttgtccaagc ctttaccttc tccgccccgg gtcctggttc gacggttcgt accgtgggcg
     4981 gcacacccag tgacaggagg actgcgatgg aacgacgcac gacgatcggg gtcgccgccg
     5041 gcatgaccct caccatcgct gcgatcgccg ttgccgtcgg gctgaacgtc gcgatcatgt
     5101 cggatcggtc ggcgacgacc ggtccgggca cgttcgaacc ggtcgccgag accagggcgc
     5161 cgtccggctc ctcggcgaac ccgacacccg cggccgctgc cgccgtcgaa acggcaccgg
     5221 cacctgcccc ggcacccacc ggggagacga ccccgccgac cgcgatctcg acatcacccg
     5281 ccccgacgtc gtcggtccac cacgacgacg atcaacgcga gggccacgac gaggatcatc
     5341 acgaggacga ggacggcgac gatgactgaa cgccgccgcc gcccgcaccc ggcgctcggg
     5401 gcccgttggg tgtccgctgg gctggccgtc gccgcgacgg tcgggatcac gagctacctc
     5461 gagatcgaca gcgtcctcca ccccaccgcg ctcgccgctg ccgccgcacc gtcggcgacc
     5521 gcacccccag cgactgcacc cccagcgacg gcgcccccgg cgacggcacc gccgaccacc
     5581 atcgcgccgg ccacggccgt gcccggcacc ggcaggatcg tcgtgccgcc acctccgccc
     5641 gtcaccgtca cgaccatccc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nccggtctac gtcccgccga cccacgcccg aacccggggc tccaacgggt gagcgtctcg
     5821 aacacgactc ggcgggcgat ggggacgacg gccgcggtga cggtccacgg cggtcgcctc
     5881 gacgccgacg acgtcctcgc cgagatcgat cggctcgagc agtcgtggag ccggttccgg
     5941 cccgacagcg aactgagccg tctcaacgac tgcggcggca gcctcaccgt cgtctcggcg
     6001 accctcctgg tgctcgttga gcacctcatg gcggcgcacc gtctgaccgg tggccgattc
     6061 gaccccacga tgggagcgca catcgcggcg ctgggttatg accgcaccta ccgcgacctg
     6121 agcgatggct cgcggcccgt tcgaccgctg cccgacgtcg tcggggccga cgacatcgtg
     6181 gtcatcccga gcacctccgc ggtactgctc ccggccggcg tccgtctcga ccccggcggt
     6241 ctcgggaagg gcctcgctgc tgacctcgcc gccacgatgg cgtgcgatgc cggcgccgac
     6301 ggcgtgctgg tcgacctcgg cggcgacatc gtcacccggg gcagcgcgcc cgacggcagc
     6361 gcctggcgaa tcgacgtgcc gtccaccggc gtgcagccgc ggcggatctt cgaactgcac
     6421 gagggtgccg tcgccacatc ggacacctcc gtgcgcacgt ggacggtcgc gggagtgcgg
     6481 caccgccacg tcctcgatcc ccacaccggc ctccccctcg aacgtgacct ccgggccacc
     6541 gtggtggccg gcgccggctg gtgggcggag gccgcggcaa ctgcggcaat catctccacg
     6601 gcgtggggcg accgatgggt ggactccctc ggaacgatcg acgggatcag cggcgtcgac
     6661 gtgtcggagc cggtggccgc cgatgcctga ccagctgtgg tggttcctcg cgcgggcgag
     6721 cggcctcgtg acgctcggag catccggtgg gtcggtgatc tggggactcc tgctctcgac
     6781 gcggttgatc cgtcgccgct cgctcccgaa gtggttgctc gacctgcacc gattcctcgg
     6841 agccctgacg atcgcgttcc tggtgctgca cgtcggggcg ctcgtcgccg acagcttcgt
     6901 gcacttcggc gtcgcggacg tcctggtgcc gttccacagc tcgtggcgca ccgtcgccgt
     6961 cgggtgggga gtgatcgccg cctacgtgct ggtcgcggtc gaggcaacat cgctcctccg
     7021 ccgccgcctc ccccgccgct ggtgggcccg cgtgcacctg tcgagcttcg tggcgttcgc
     7081 gttgtcggtg ctccacgcgg tgaccgccgg gaccgaccgg tcgaaccggc tgttcgtcgt
     7141 cctgacgttc gccatgtgcg ccgtcgtcgt cttcctcacg ctcgtccgca tcctgagcgc
     7201 ctcgtcgtca ccgacgtcgc aacctttgcc gtcccctaac ccgtgacacc ccctctgttg
     7261 gcacgaccgt ccgtaccctg gatcccagat ccccagcacc cggagccctt gctgctcctc
     7321 catcacgaag gctgcccatg accgagaccg aagccagcgc accgaagaag aagtacttca
     7381 cctcgtggtg gtcgaaggtc ctcctcctcg tcggcatcac cgcggtcgcg atccagttcg
     7441 tcccgttcgg ggtcgacaac ccgtcggcac gcgacgaacc gcagtgggac agcccgacca
     7501 cccggcagct cttcatgacc gcctgcgccg actgccacag caacgagacc aaggtgctgt
     7561 ggttcgagca cgttgcgccc atcaagtggt acatcgccga ccacgtgaag gagggacgtg
     7621 aggcgctcaa cgtcagcgag tggcacacca accccgggcg tgacaccgac gagttcgccg
     7681 aggtcgtcga ggagggctcg atgccacccg actactacac gtacttcggc ctgcacagtg
     7741 actcgaagct caccgagacc gagaagcagc agctgatcga cggcatcgag aagaccctcg
     7801 ccgccgaccc gccgacctcg ggccgataga ccccgcaacc ggcaccgatc aggcggccga
     7861 gattccccag accagggctg caccgacgag cccggcaccg acggcgacga cgctcacgag
     7921 caaccggagt tccccgaaca gcgaaccgag cgccgggtcg ctgaagcggg caacggtcgg
     7981 ttcgtccgac ggcggagccg acccggcatc ggggcggagg atcgtccgga gcgcgagcac
     8041 cccggcgacc acgaccgccg agccgagtcc gcccacgagc atcgggccag ccgcgcccac
     8101 catctcaggc caccttcgag gtggtcggtg ccgtgaacgg acggggtgtc gaggtcatgg
     8161 cacggaccgt accgacacca cgatctgcgc cgacccaagc gacggtgagc gcacggttaa
     8221 tcctcgatgg aacccgaacc gagcgctgcg cgacgccggt gagccgtccg ctcacccgaa
     8281 aactcccggc ggccgaccgt cggtccggca gacttggacc atgcggccat gccgacgagg
     8341 tttcgaatga ccgacgagtt gctcatcgtc gaggacgatc ccggtatccg gtccggtctc
     8401 gtccgggcgc tcaccaacgc cggctatgcc tgtcgtcccg ccccggacgc cgcggcggcg
     8461 cgtgcggtga tcgaccctcc gggcctggtg ctgctcgacc tcggcctccc cgatgccgac
     8521 ggcctcgacc tcgcccgcga actccacgac cgctggccgg acgtgccgat cgtgatggtc
     8581 accgctcgcg ccgaggagat ggacgtcgtg gtcggcctcg acagcggcgc catcgactac
     8641 gtcaccaagc cgttccggct ggcagagctc ctcgcgagga tccgcgccca cctgcggcgc
     8701 cccgcgcccg ccgccgacgc tcctgccctc gtcggtgccg gggtgatcgt cgatctcggg
     8761 gcgcgcatcg tgcggctgga cggtgaggag atctccctcc gggccaagga gttcgacctc
     8821 ctcgtggcgc tgatgagcaa tcagggcaac gtcgtcaccc gcgaagaact catggcccag
     8881 gtgtgggacg agcactggtt cgggtcgacc aagacactgg atgtccacat ctcgtccctg
     8941 cggcgccggc tcggggaagg gcccggcgaa ccgagtcgca tcaccgcgtt gcgcggcgtc
     9001 gggtaccgat gggagcgaga tgcctgaccg caacggtgtg ttccggtgag gcgtcggatc
     9061 ctcacggcga tcgtgggggt caccgcgctg gcgaccatcg tgctcacgct tcccctcgtg
     9121 ttcctcatgg ccagccggga gcacaacgac tccgaccgcg agctcacgct cgtcgccgag
     9181 caggtcgcca acgagctcac ctccgactcg gacttctccg ccagcggact cgagctgcct
     9241 ccgatcgacg acgccctgat caccgtcggc gtgtatctcc ccgacggtga gctcgtcgcc
     9301 ggcgatggtc cgacgcccgc cgacgagttg acccggtcgg ccggactcct gtcgagcctc
     9361 gacaacgtcg acgaccaccg cgtgctcgtc cggccgatcg tcgagcgcga gcagaaggtg
     9421 gcggtgatcc gcgtggccga gccgctctcg gaagcggaac gcaagatctg gcgcgacatc
     9481 ctcgtcctcg tcgggatcga cgctgcggcg ctgatcgtcg ccgcggtcgt cggctcgtgg
     9541 gtgacctcgc gcctcgtccg ccccctgcga gcgatccgag acgacgcagt ccgcctcggc
     9601 gagggggact tctccatcgt gccgacgccg agcgggatcg acgaactcga tgccaccgcc
     9661 ggcgcactcg ccgatacggc agcgcgcctc gatggtgtcc tccagcgcga gcgatccttc
     9721 accgccgacg catcccatca gctccggacg ccgctcacgt cgctccggct cgtgatcgaa
     9781 accgagctgc agtcgccggg agccgacccg acgccggtgc tcgacgaggc gctcggtgag
     9841 atcgatcggt tggagcgcac gatcaccacg ctcctgcgcg tggcgcgcga ccaaccgatc
     9901 acccgtgctc cactcgacat cgaccagtgg ctcggcaccg tcgtcgggcg gtggcaggcg
     9961 gtgttcgacg agcggggccg ctcgatcacc ggcaccaccg gcgtcggcgg cagcccccgc
    10021 gtgagcctct ccgtgctcga ccagatcgcc gacatcctcc tgcagaacgc ggtgcagcac
    10081 ggccgcggtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcgggcggg
    10201 ggcttcgggc cgagctgacg gcacgcggcc gcggtcgcct cgtcgaaggg gaaccacgtc
    10261 tcgatacgga ggttctcgac cgccacgttc tgcggcgcct ggaaggcggt gatcgtcgtg
    10321 aggaaccgga gatcgaggcc ctggccacgg aggtggagca caagcgccgg atcggacggt
    10381 cgttgcaggt ccggctggcg ccagtcgggg tcgatggtcg gcagcgcgag gaggtcgtcg
    10441 aggaggcggc tgagctcgtc gtcgccggga tcggcgagcg cttctcgttg gatccgccac
    10501 aggaggtccc tgccgacctc ggcgaagttc gcgatccacg gctgcgcgcc gagcggatcg
    10561 aacgtgaggc gagcgaggtt cacccccgct ccgatgacgt cgttgaactc gcccgacggc
    10621 tcggcggcgc cgctgccgag caggaccccg aacagcccca gcgccccctc gttgaggtcg
    10681 aggacccggt aggagcggtc gaccacgacg agcgggaacg gctcgtggtg ggccttgagc
    10741 aacgtcagcg cgtcggtgac aacggccggg aggacgtcgt cggactcgtc gtagatcggg
    10801 tcgtggccgg cggcgcgcag catcgcgttc acctgccgga gcgggagcgc cagggcgagc
    10861 gccagctgca ccaccatctc ggggctcgga cgcgaacgcc ccgtctcgag gaagctgacg
    10921 tgccgcgccg acacatcggc gatgagcgcc aggtcgagct gactcatgcc acgccgccgg
    10981 cgccagtgct tgagcagcgc cgggaagagc ccggagcggc ggtcgagatc gggcatcgtt
    11041 cctcgcagct cgatgaggtc gttgggagtc ctcaccgtac ggcgaggtgg tcacgggggc
    11101 acctacctcc tcagttcgtt gcggtggcgg gcccggcagc gcatcgtcgt ggacgccggc
    11161 ccgaccggga cggtgacgac gctccaggag ccccgatgtc cacactcacg cccgccgaag
    11221 cgaccgacct cttccgtgcc gccccacacc gatacctcga cgtgggcgcc ggacaggcgg
    11281 cctaccgtcg ggtcggtacc gggcccgacg tcctgttcgt ccacggatgg cccgtcagcg
    11341 gggcgacgtt ccgtcggctc ctcccgcacc tcgccgacca cgtcacctgc cacgtcatcg
    11401 acctgccgag cagcgggtcg agccgcttcg acgacacgac gccgctgtcg atcgagcagc
    11461 acatcacgac ggtgcgacgg gtcgtcgatc tgctcgagct cccgtcgttc gccgcggtcg
    11521 gccacgacag cggcgggctc atcgcccgcc acgcgttcgc cggcgacccc cgcctgaggg
    11581 cgctggggct cgtcaacacc gagcagcccc agggcttgag ccgacgattc cggctgttcc
    11641 tcgccggccg ccggcttccg gggttcggcg ccgcgctggg ttggctcgct gggcggccga
    11701 agctccggcg caacggattc gtgctgggcg acgcgttcgc cgaccggtcc ctgctcgacg
    11761 gcgagttcga cgagttcttc ctgcgtcccc ttcacgagat cccgtcgcga cgccgagcgg
    11821 cgatccgcgt gctccgcacg ttcgacgagc gatacgtgct cgagctgggt gcgctgcatc
    11881 gccgcatcga cgtgccggtg cagctggtgt ggggcgacca ggatccgttc ttcccggtcg
    11941 cgtgggccga ggagatggcg gggacgttcc ccgacgcacg tctgacggtc gtcgaggggg
    12001 ccgggctctt cagccacgag gagcggccga ccgaggtcgc cgccgccctg cttccggtgt
    12061 tgacaggtca ccctcacgac gccgtgggct gaggcccgac cgcgtggtcg ccaccaccgg
    12121 acaacgcttt cttttttcaa gtactctgtc cggatcggca cggacgcagc gtcggtggcg
    12181 tgatccggag ggaacggccc ggcggatctg accagaggag tcgcgatgac cacccagcac
    12241 atccagatcg ccatcaaggc gacgcccgag gcggtctgga ccgccctgac cgacggagcc
    12301 atcacccccg cgtactacgt ggggttcgag gcgaacttcg acctcacccc cggcgcggcc
    12361 tactcctaca ccgctggtgg cggcgacatg atcaccggcg tggtcaccgc cgtcgagccg
    12421 gcatcgacgc tcaccacgac cttcaacggc cactgggacc cggccgtcgc cgacctgccc
    12481 gaatcgaccg tcacgttcac actcggcgcg ccggccatgc cgctgcccgg catcacggtc
    12541 ctgtcctgcc gtcacgaggg tctcgacgac tcccccgccg ccgcggcgct ggagtcggga
    12601 tgggtcacca tcctgtccgg cctcaagacc ctgctcgaga ccggccgtcc catggtcgac
    12661 gtcgcctgag cggtcgagcg aagcagtcac gccgatacgt tgtcggcatg aagtcgtacc
    12721 agcagtactg cggcctcgcc cgaggcctcg acctcgtcgg cgatcggtgg acgctgctcg
    12781 tcatccgcga gctcgcgctg ggcgaccgcc ggtacgccca gctgcagcgg gacctccccg
    12841 gcatccccac gaacctcctg gctgaccgtc tccggtccct caccgcgtcc ggcctgatcg
    12901 agccgacggc gggaacggcc cgctcgggcg gttacgcgct cacgccccgg ggccgggaga
    12961 tcctgcccgt cctccacgcg ctcgtgcgct ggtccacgcc gaccatgctc gacgggccgg
    13021 acgccgacga ccaccagagc gcgaggtggg tcgcattcgc cgccatggcc tacctccggt
    13081 cggctccggc ggggtcgccg gtgtcgatca agctgtcgtg cggggcggag cacgtcgtca
    13141 tccgagccga tcacgacgtg acgatcagcc tcgacgacga gctcccgacc gacgtcacca
    13201 tcgacggcaa cgcgtgggcc ctcatgggca tcgtgtcggg agcgctcccg atcgggcggg
    13261 tacgggcggt cgacgactcg gctgcgatcg tgggcaccca ggcgtcgcgg cgtcgggcga
    13321 ccgccctgat cgcgcacaac accgcggcgg cctcgtgaac gccggcgccg gcgagcccgg
    13381 cgtcggcgac cgctaccgcg atgcgcgcct gcggatcacc gccctgctcg cacggtcgct
    13441 caccggcggg attcccgatg acgtgttcgt tgacctcgac gagggctgac gaacccacgc
    13501 ccggcctcac gctccgtgcc cacgccaccg tgcccgcacg tgaccgatga cgcggacggg
    13561 cccgggtcga atccgacccg ggcccgtcga tgcaggtacg ttcggggccc gatggccccg
    13621 gtcgtcatca gccgttggcg gcggtgatca ccgagtcgag gacgtggatc acaccgttgg
    13681 aggcaacgat gtcggtcgtc acgaccttgg cgccaccaac ggtgacctcg ccaccgttga
    13741 cctcgacctt gagcttgccg ccaccgaggg tctcgatgga ggtgccggcc gcggccacgg
    13801 cgtcagcggc catgatcttg ccgctcacga cgtggagctt cagcacgtcg gcgagggctc
    13861 cggtcgggtc ggcggcgagc gcctcgagcg tcgcagcatc gaccttggcg aaggcctcgt
    13921 tggtcggggc gaacacggtg aaggggccct caccgctcaa cgcctcggcg aggccggcct
    13981 tgcccacgag gtcgacgagg gtcgagaagt ccgggttgcc ggcggcgatg tcgacgatcg
    14041 tccccgacgc ctcgggcgcc tcggtcgtgg ccggagcctc ggtggtgtcg ggtgcctcgg
    14101 tcgtcgccgg tgcctcggtg gtcgtggtgt cgtcggcggt gtcggagtca tccgagcagc
    14161 cggcggcgag caacgcgacc gggagggtca gaacggcgag ggcacgagaa atacgcatgg
    14221 caaagctcct tgggagatgg attgcccgta cttcgttcgg acccgccggt ccggatgcac
    14281 gaatcttcgc gacggcgtgg ttcagccgtc gagacgaccg tcgcggatgt ggaccgtgcg
    14341 gtcggcgtag tgggtgatgc gatcgtcgtg ggtcacgatg atcgccgccg tgccccgcga
    14401 cttcatctcg ttctggatga gctccatcac ctgctcaccg agcttggagt cgagcgcgct
    14461 cgtcggttcg tcgaacagca ccagcgtggg ttcgttcatc agcgctcggc cgatcgcgac
    14521 ccgctgacgc tggccacccg acagctgctc ggggaggttg ttgcgtcgat cggtcaggcc
    14581 cagctcctcg agcagcgtgt cggcccggtc acgggccgcc tggccggtcc gtcggccgag
    14641 ctcgtcgacg acgagcaggt tctcacgagc ggtcaggaac gggatcaggt tcaccgactg
    14701 gaacacgaag ccgatgttgc tctggcggaa ctcggtgagt tggcggtcgg agtacccgct
    14761 gatgctcgtg cccgcgaccg tgacctcgcc cgacgtcgga gcgaggatgc cgccggcaat
    14821 ggaacacaag gtcgtcttgc ccgaaccgga cgggccgatg agggcgacga tctcgtcgga
    14881 cccgaccgtc aggtcgatgt cggcgagcgc aacgatctcg tcgtcaccga cctggtaggt
    14941 cttcgtgacg tcgtcgaggt ggagggcggg gtggtcggtc atgtcgaggt cccgatggct
    15001 gaggctggat cgatacggag cacgcgtcgg agcgagaacg atgcgccgag caccgacgcg
    15061 aggaggagta gagcgagtga ggttccgacc cgtgcggggg tcagggtgaa ggggatgctg
    15121 tcggcgggca gggccgcctc gaacacgatc gcgaccacgc tggcgatgac gccggcgatc
    15181 aacgacagga cgacggcctg ggtgatcacc gccgcgaaga tcgagcggtt gcgggcgccg
    15241 atcgccttga gcacgccgta caggccgacc cgctcgccgg tgagcagcgc gaagaacaga
    15301 gcgaccacgg cagcagcgat gaccacggtc agaccgatga tctgctggag cacgccgccc
    15361 cccgtgtcgg ggatcgcgtc ggcggcggcg gcccgggtca ccgtcgacgt ggaccccgtc
    15421 gcttcgtcga tggcacgtgc ggtcgctgcg ggtgtcgagg tgtcgacgac cacgacctgg
    15481 gtggcaccct cggggagcac ggcgtcgggc ttgttggccg cgacgacctc acggagcgac
    15541 gccgacgaca cgagcatcgt cggctgggtc tggaagccga cgtcgtcggc gaagcccgcg
    15601 acccgaagcg gggtccgggc ggcgccgacc tcgatcacgg tgccgacccg cacaccgcgg
    15661 tcgcgaaggg tcgaatcgac ccaggcatca ccgtcgggca tcgagtccgg caggccggcg
    15721 aacggacggt cgtagccgac gacgacgacg tcgatgaggt cacggggtgc cgcgtcgggc
    15781 agccggccgc cgagctgcac caggccgagc gaaccggtcg ctgcgacacc gtcggtcgca
    15841 gcgatcgccg cgacggtgtc ggcggggacg aggctctggg ggatcgactc ctccgaggtc
    15901 gccgagaaca cgatgaggtc ggcgctctgg gagcgcagaa gaccgtcgtt gttggcgttg
    15961 agcccctcga cgaggccgct cagcaggagc agcagcgagc cgagcaggaa gaggatcgcc
    16021 gtggccgcag cgaagcggcc gggacgtcga cgcaattcgc gccatgcgag tttcatcggc
    16081 ccgcacctcc cccgccggtt gcctggttcg ggtcgatcgc caggacgcgg cggatggacg
    16141 cgacggccga caccaccccg agcaccacga gggtcgtcac ccagaacccc acgttcgtcg
    16201 gctcgaaccg gagccggagg ctgcccgtct ttcctcgtgt cagcagggcg aacccgccga
    16261 ccgcgatgcc gagcccgacg ccgagcacga gcgacacctg gacgaggagc gcctccacca
    16321 ggcgacgggc cggagctccg agggcccgga gcagcacgag ggaactggct ttctggaagg
    16381 tgaggatcag gaagaacagc ccgcacacga gtgggaccac gagtccgaag aggacgagga
    16441 tcaccgtgaa cgactgcttg atcgactcga tgccggggtt gagttcggcg gcgtcgacgc
    16501 gcgacagcgc gtcgatcgat tcgtcggcac ggttgagccg cgtggtgagc tcggcgaccg
    16561 tcgtgccact ggcgggcgac acgcccagca cgttgggcac gtccaacgtc gcggccggat
    16621 tccgcgtctg caggacggtg aggtacgtgt ccgccgtcgt gaaaacggtc ggcagcacgt
    16681 tgatgccgac gtcgtcggcg agcccgacga cctcgagatc gatcccggcc ggttgcaccg
    16741 tcacggtgga cccgatgccg aagccgtcct cttcgttgcc ggcgttggcg acgacctcgc
    16801 cgggggcctc ggggagacgg ccttcgagca gcgggccggg gccgccgagg tcggggtcgg
    16861 cgtagccgag caccgaggtc gcctgggtgt caccaccggc ctcgatcggg aacgtcccct
    16921 ggtagatggc agcgatccca ccgatgccct cggtcgcccg cactttctcc tcgagttcgg
    16981 ggctcacgga gctgctctgg ggaaaccggc gcccgtcgac gttgaacacg agcacgggcg
    17041 cggtctggtg acgcacgccg ccgacgaact gccggatcag ggagttctgg atcgccgatt
    17101 ggaacaggat caggaacgcc agcagcgcaa cggcggcgat caggagaccg aagcggacct
    17161 ttgatcttcg aatctcacgg agagccaaga acacgacgca gtttcctccc cgaacgggtc
    17221 aattcccaaa ccgaagtcga cgctcgagcg gccagcaccg gatgggcgga acagtggtac
    17281 cgcggtcctt cgttcggacc cgtcgattcg gatgcacctg gttgcccgga gagcagatcg
    17341 tggatcgtcc ggcgctgaac gcggacggag ggcccaacga atgggccctc cgattacgag
    17401 cgacgactcg gttggtccgg ccggtggtgc ggccggaccg gcgcgatcat gcagccggcg
    17461 tcggttgctc gatcgacatg cgttcggcac tcgacgcgtc gagcgccgac ggttcgaccg
    17521 gggccgtggc gatgctgatc ttgcgaggct tggcgcgctc ggagatgggc acccgcaaca
    17581 cgaggacccc ggcgtcgtag ccggcacgga cccgatcggt gtcgagtgtg tcgccgagaa
    17641 cgatctgccg actgaacaca ccgcgaggtc gctcggcggc gacgtagtcg cgatgcccct
    17701 cgggttgggg acgctcggcg cggaccgtga gcacgttgcg ctcgacgtcg atgtcgatcg
    17761 agtcggcgct cacccccgga atgtccagct cgatgacgaa ctcgtcaccg tcgcgccacg
    17821 catccatcgc catggcggcg ggacgagccg ccgtgcccag gagctgctgg gtgagccggt
    17881 cgagatcacg gaacggatcg gtgcgcatca acatggttcg ttgcctcctg acgtgacgtt
    17941 gggatgaatc gacagtcact cattgcttat ctgttgtgac tgccatagat ttaggatatt
    18001 caaatcggcg acaggttgca accagttcgg agcggttcca tggccaacgg cgaagacgat
    18061 ccgacgaggg gtctctacgg gatctcggtc gcggccgagc tggtcggcaa cggcgagcag
    18121 aacattcgcc tctacgagcg gcgtggcctc gtgaccccgg cccgatccgc cggcggcacc
    18181 cgtcgctaca gcgaccacga cctcggtcgg ctgcggcgga tcgccgaact cctggccgac
    18241 gggctgaacc tggccggggt gggccgggtg ctcgagctcg aggacgagat cggtcggctc
    18301 cgggccgaac gagacgccgg cgaccggcgc tgacgccctc ggcgtcaggt tcggtcgggc
    18361 cagtgcaggc cctcgcgccg gatcgcctcg tcgagggcat cgacgaggcg atcgaccgac
    18421 cccgcgcacg cgtcgaggtc ggccggcatc aggaggtgca cgatgccgtc ggtggtctcg
    18481 gcgacgactc cgggagcaac gccgcccgtc gcggcgcgaa cctcgggcgg ttggtcgtcg
    18541 cggtggtacg cgtcgaacgg cacggggagg ccgtcgcggc acgtccgcca ctcggatcgt
    18601 tcgcggacga gcccgtgggt gatgtcgcag agcgagcagt gcgcccgtcc gaggcgggca
    18661 ccgacccagt aggtcagttc gccccggagc gagccgtcgg cgtcgtacac cccgacgagc
    18721 ctgacgatgt gggtcggggc gtcggtggac ggcacgtcgg aacggtcggt cggcacgcgc
    18781 tcagggtacg gcaccgcggt cccgggcccc cgccctccgc gatcgggtgg ccacgagcgc
    18841 cgtcgacacc accagcacac ctgccggcag gaagagcagc cacttcgagg aaccgagcgc
    18901 cgcggcgacc gcggcgccgg cgacgtagct gaccagcacc gtggccagca ccaggacggt
    18961 gacccgtcgt cgatgcgccg aggtccgatc ggcccgccgt ccggcgagcg ccactttctc
    19021 gccgatgcgc acgatcgccc cggtcccgta ggtcgtcgag accgcgacgt cgcccacacg
    19081 gcggagggtc gccgcctgga cgcccatcgc cgcagcggcg accaccacga tcggtatggc
    19141 cgaaccggtg agccggggat ggaagccgac gccgtgccga ctccggagca ccgcaaccgc
    19201 cacgagcagc accgactcga cggccaggag cacttcgggg cgcaggtggc cccaccgtcg
    19261 cagtcggcgg tcgtgctcga cgatcgagac gacgacaccg gcggcgaagg ccacgaccgc
    19321 cgctgccgcg ccgaccgcct gcccccagtt gcggaccccg gtcagcatgc cgatacggac
    19381 gagattgccg ctcatgttcg ccacgaagat cggcgccacc gcgaggtaga tgtgggcgtc
    19441 gacgaacccg gcaaccgccg ccagcgcgac ggcgagcagg agaggacccg aggggtggtg
    19501 cgtcgactcc tgggtcaccg tgtactcagg cggccgtgac gaggtcggcg ccgacgagtc
    19561 cggccgcgac gtcccgcatg atgtcaccgg cggtgttgac gggttctggt tgcgagttca
    19621 tagcggctcc gatggtcggg cgacccgggc gcgggcctgg cccgaccctg cgccccgtgt
    19681 cggcgccata ccagtgccga acgtcccgag cgtaccgctc tcacccgtct gtccagcgcg
    19741 cccgttctgt cccgcgaacg cgtgcagtcc ggatcccctc gcgggacaga acgagtcgca
    19801 gcgctatgac ccgacggggg tgaaccgtgc cggcaaccga tggatgccgg cgacgaaggc
    19861 cgacgcgctc cggtcgaccg gtccggtcgg atcggtcgcg gcgatgtcgg gcaaccgacg
    19921 gaacagctcc tcgaagacga ccttgatctc catccgggcg aggttcgccc ccatgcagaa
    19981 atggctgccg aacccgaatg caatgtgggg gttcgggctc cggtcgatgc ggaacgtgtc
    20041 gggatcctcg aacaccgact cgtcgcggtt ggccgattgg tacaacagga gcacctggtc
    20101 accggccgac aactccactc ccccgagggc caccggctcg gtgacggtgc gggtgaagtt
    20161 gagcaccgga gaggtccatc gcaggatctc ctcgacggca ccgtcgatca gtgacggatc
    20221 ctgcaccagg cgatcgcgtt cgccggggaa ctggctgaag gcccggaggc cgcccgcgat
    20281 ggcgttacga gtcgtctcgt tgccggcgac catcaacagc acgcagaaca tcatcagctc
    20341 gtcgttggtg aggtcggaga cgtcggcacc ctcggcgccg gcctgggcgt cctcgtagcg
    20401 cgccttggtc gcatcgtcgt aggccagctc gccgctgtcg tacgcggcgg tcagcaccga
    20461 gatcagatcg cccttcgggt cggtccgtcg ctcggcgatc agctcaccga tcagggtcgt
    20521 gtactcgacg aacgcgatcg cggcacggtc ggtcgccggt ccgggggcgc cggcggtcgc
    20581 ttcgccgccg atcatggcat cggaccagtc gtagagcgcg ttgcgcatcc ccgggtcgat
    20641 gccgaggagc tcggagatca cgatgagggg cacgtgcatc gcgaggtccg acacgaagtc
    20701 gatctccccc gatgcctcca cctcgtcgat gacctggttg gtgagctcgc gcacgtggtc
    20761 ggtcatcgag cggacctgcc ggggcgtgaa ccccttcgag agcacccgac gctggcgggc
    20821 atgctcggga tcgtccatcg agacgatcga gagcggtacg aggttgaccg gtcgcactcc
    20881 cgctgccgag ctgaagtacg tggagtcccg cgacgccgtc accacgtccg cgtggcgtga
    20941 caccgcgtag aggcccgagc gctcgtcgaa ccacaccggg tgctcggcgc ggagccacgc
    21001 gtagaacgcc cagggatcgt cgtagggggt cgggtcggtc aggtcggtcg acgcgaagtc
    21061 gaggggggaa tccgacaccg tcagtccttc cggtcctcgg gccacagcgg cgccgcggtc
    21121 tggttcaccg ggtagtggcc cggctgcttg ccgagggcac ggtgcatccg cgacgtcatg
    21181 ccttccgaca ggacgtcggc cgacatcatc tccatgaaga gcgaaccggc gttgccgagg
    21241 tcgaaccaat cacgctgcca ggcccactcc atgttgccgg cgtacttgaa ccagctgccg
    21301 ccgatgccgg cgatctcgta ggacgatccg tcgggtcgct tggcgtcggc gacctgccgc
    21361 cagaggccga tgacctggcc gatcttctcg tcgatgacca cggtctcgta ggggtaggtc
    21421 cagccgtcga ggccgtccat ctccaggccg atcgcccagt cacggatctg gtcgcgaccg
    21481 accgccatga agttgacctc ggggccgtag ttccacccgt aggtcgcgtc ctcggcgtag
    21541 cactcggcca tcacccgcca gtcgcccgac gcctccgcct gtcgattgac gtcgagccac
    21601 ttctcgacca tcgcttcgag ttcttctctc ggaaatgctg gcatccgtcc tcccgggtcc
    21661 tgagcgccgg gtcggacagt aacggatggg cggcgccgaa ctcgtcggag cgcgccgttc
    21721 tcggagccgg gtcgactgcc gggacggaga aggatcagtc cgacaacgcc ccgccggcgc
    21781 caaccgtgac cccccgcccg gcgctgaccg gtatcgcacc tcaggaccgg gtcaggacca
    21841 ccttgagtgc ccccgtgtcg gcggcccggg agaacacgtc gtaggcctcg tcgaactggt
    21901 cgagggtgaa gtggtgcgtg atgaaccggt ccacctccat ctggtggccc tgcgcgagtc
    21961 gcagcagggt cggggtggag gaggtgtcga cgaggccggt cgtgatggtg acgttgcgga
    22021 tccagagatc ctcgaggtgg agcgtcgccg gttcaccgtg cacaccgatg ttggcgatgt
    22081 gcccgcccgg ccgagcaagc ctcgtggcca actcgaaggt cgccggcact ccgacggcct
    22141 cgatcgcgac gtcggcgccg agcccgtcgg tcatcgccat gacctcggcc actgcgtcgg
    22201 tgtggctgtt gttgatcacg acgtcggcgc cgaactgctt ggcagcgtcg agtcgggtgt
    22261 cggccaagtc gatcgcgatg atgcggctcg ggctgaacag cttcgaaccc atgatcgcgg
    22321 agagcccgat gggcccggcc ccgacgaccg cgaccacgtc cccgggtcga acctggccgt
    22381 tcaggacgcc cacctcgtag cccgtcggga ggatgtcggc cagcatcagg atctgctcgt
    22441 cggtcacccc gtcggggatc gggtaggtcg acgtgtcggc gaacggcacg cggaccttct
    22501 cggcctgcgt cccgtcgatc ttgtgcccga ggatccaccc gccgccgccg aggcattggc
    22561 tgtagctgcc ctcgcggcag tactggcacg caccgcacgc ggtgatgcac gacacgagga
    22621 cacgatcgcc gaccttgacg ttcttcacac cggcgcccac cgtctcgacg gtgcccacgg
    22681 cttcgtgacc gaggatgcga ccgtcggtca cggcggggac gtcgcccttg aggatgtgca
    22741 ggtcggtgcc gcagatcgtg accgcgtcga cgcggatgat ggcgtcggtg tcgtcgatga
    22801 cgacggggtc gggcacatcc tcccacgcct tgtggccggg accgtggtaa acgatggcct
    22861 tcacggcgac tccttgaggg tcgagggaac ctgattcgac cgtacgcccg cggtggcgtg
    22921 gcgggcagag gacaaggtcc ctcccgagtt cccaaccggg agagcgagcg ccggagatca
    22981 ggcccagcga agggtgacga ccgatcgtgc cgggagcgtc accgcgaccg accgatcgcc
    23041 gtcggtcacc gcgacgtcga ccggctggct cgcggtcgag acgaggaacg cgacggtcga
    23101 gccgtcgggg ttctcgaaca cgacgttgtg gaccccgccg gttgccgagg acgagccgat
    23161 ccgggtcgcc ccgacgtcga cgcctcgccc gaactgcgcg agcgcgtaga actcctcgtt
    23221 gcgggtgacg tgcccgtcgg gatggatggt caggaccccg cgacaattgc ggcagcctcc
    23281 gacgcggggt ccgctccgtt cgtcgagcgc gaggttccag agcacgaccg accgggacca
    23341 gttgcgcacc gagtcgacga ggagcacgcg ggcgttccac aggaggttgg actcgaaggt
    23401 gccgctcgac tcggtgccgg tgcactcggt gaagtggatg tcgaggtcgg ggtacgcgtt
    23461 gtgcaccgtc gactgcgccg acgggttgcc cgagtagcag tggaacgccg acccggcgat
    23521 ccgtggagtg ccggcgacgc gtgccagcac gtcgagggcg cggggtgcgc cgttccagtt
    23581 gtggtcgtgg acgagcagct cggtcgacga cccgtcggtt tggagcccgt cggcgagttc
    23641 gatcgcgagg tcggcctcct gctcgggcga caggaccatg cccgcgtagt tgtcggtctc
    23701 gtgggccggc tcgttctgga ccgtgaggag cgcgagcggg accccggcgt cggcgtaccc
    23761 gtcgacgacc cgccgcaggt accgcgagta cacctcggtc atgccgtcgg cgagcgtgcc
    23821 gccgacgacg cccaagcggt cggccgaggc gccggacttc atccagcccg gcgctgtcca
    23881 cgccgagccg atgagggcga gatcggggtt gacggccagg gcggcgcgga ggagcgggaa
    23941 gcgctgctgt tcctcacggg cgagcgagaa cgcggtgagg tcgggatcct cgcccggcag
    24001 ctcgatgtcg tcgtaggtga agtcgtcgag cgagaagtcg gtcgacgaga gcggcacccg
    24061 caccacgctg aggttggcgc cgcgccggcc gaagaggtcg ttgatcacct gggtccgggt
    24121 cacgtcgttc aaccgggtcg agagcaccca cgctgacgag tcggtcatgg ccgcgccgac
    24181 gccggccatc gactggtagc gggcggaccg gtcgatgtcg accaccaccg ctgccgcgcc
    24241 gggaccgttg gcgatcggta cccgaggttc gaccgagagc agcttcgcgc cgtcgccggt
    24301 cgtgacgagg acgttggcgt atccgtcgac gagctgttcg gcgccggtgt ccaccgcggg
    24361 tgagtcggga ccgcacgacg ccacgatcga cgcgctcaac aacagtccga gtccggtgcc
    24421 gatcatccgc cgcatgtcgt tgctctccgc tcgccccgtg gcctcccgcc caaggcctgc
    24481 gaccacgata gaacgacatc tccgcccggc accgcttcag cgcgaacatc gagtcggccg
    24541 ccgcgactcg ttcggggcgc ttggcatgcg aagttaagct cacctactgt gcggccggtg
    24601 ggcagcatga cgcgtggagg tcggtggcgt tccggccggt tgctcgtcgt cgccctcctc
    24661 gcggtgtggg gcgtcggggc gttggtcggc ggggcactcc ccgccggtgc tcccgccgat
    24721 caagccgccg ggtccgaact cgagtcggtg gtcggtcacc gagcggcggt gacggtcctc
    24781 gcagcgcgcg ctccccggtc cggtgacgag ctcgcgagcg aatcggtcga cgacgcaccg
    24841 tcggccgatc gagcggtgcc gaccgtcggg ttcgccgcca cgccgctgcg cgcgacgggt
    24901 ccggctccgg gtcgggctcc accgaacggt cggtaggccc agctcgccat ccgttcgtcc
    24961 gcgcccagcg gcaccaccac ctggagaccc ctcatgacgt caccgcgtcg atatgcactg
    25021 ctcgccctgc ttccgctcac cgccctgctc gccgcctgcg gcgacgacac ctccaccgcc
    25081 gacgacgccc ctgacggcac cgccggcgac gtgtcgggcc agaccatcac gctctactcc
    25141 ggccgcgacg aggaactcat cgcgcccctc atcgagaagt tcaccgacga caccggcatc
    25201 gaggtcgacg tccgctacgg caactccgcc gagatgggcg cccagctgct cgaggagggc
    25261 gaggacaccc ctgccgacgt gttctactcc caggaggtcg gggcggtcgg tgtgctcgcc
    25321 gaggccgggc tcctcgcctc gctgcccaac gagctggtgg ggacggtcga cgagcggttc
    25381 cgccccgacg gcgacaacga gtgggtcggc gtcaccggcc gctcccgcgt catcgtctac
    25441 aacccgaacc tgaccgacgc ccccgtcggc gtcgaagagc tcactgaccc caagtaccgg
    25501 ggtcaggtgg cgatcgtgcc gtcgaacgcc ggcttccagg cgttcatcac cggcttccgg
    25561 gccagccagg gcgaggacgc cgctcggacg tggctcgagg acctgatcgc caacgagccg
    25621 atcaccgaca tcgccagcaa cggggacgtg ctcgaagcag tcgaggccgg tgacgtcgcg
    25681 atcggtctca tcaaccacta ctactgggcc cgcgacgagc ggcagcccaa cctcac
//
LOCUS       DAOSOA010000013        24949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_2004_length_24949_cov_6.952760, whole genome shotgun sequence.
ACCESSION   DAOSOA010000013 DAOSOA010000000
VERSION     DAOSOA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 24949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24949
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_24949_cov_6.952760"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKY13_02055"
     CDS             complement(<1..837)
                     /locus_tag="PKY13_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HOT78735.1"
                     /translation="MARRRHLPGHLRRGAACDPRHPRSDRGPRVRLALSAYEVAVARL
                     RRAGCVSAEDEAAELIKVAPDDATLDGWLARRETGEPLQWITGRFRFCDRWYTIDPGV
                     YVPRPQTEELARRAAALLPEGGAALDVCTGAGAIAAHSAAEVPSATVIGVDIDPRAVG
                     CARRNGVLAVVGDLVTPIRDSPRFDLITAVAPYVPTEAIAFLPADVQRHEPRLALDGG
                     ADGTELVGRIVSAAGRLLHPGGHLLVEIGGVQHASLVGPLAAAGFGEAAPWVDEDGDL
                     RGL"
     gene            complement(735..1217)
                     /locus_tag="PKY13_02060"
     CDS             complement(735..1217)
                     /locus_tag="PKY13_02060"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT78736.1"
                     /translation="MTQERIEVQRTIPAPPAAIFAVLCDPQGHVAIDSSGMLMSADGD
                     PVSAVGDTFVVHMDREALNDFPLGHYDVTVEIHTFEPDREIAWQIRGQIKPPIDHIYG
                     YRLEPAEDGATLVTSYYDWSEIDQVWRDAGIFPVISEGALRATLGILGRTVVRGYVSP
                     "
     gene            complement(1289..1921)
                     /gene="folE"
                     /locus_tag="PKY13_02065"
     CDS             complement(1289..1921)
                     /gene="folE"
                     /locus_tag="PKY13_02065"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOT78737.1"
                     /translation="MSESGQGAHDLNRIDPSTTEAAPPVDVERIAAAVREILAAIGED
                     PERDGLKDTPARVARMYAETCAGLHEDPARHLKVTFDADHDEMILVREIPFYSMCEHH
                     LVPFFGHAHVAYIPSANGRITGLSKLARLVDGYAKRPQVQERLTTQVAQAMESALEPR
                     GIVVVIEAEHLCMGMRGVRKPGSTTVTSAVRGIFRTDPSARAEAMRFIQG"
     gene            complement(1914..3746)
                     /gene="ftsH"
                     /locus_tag="PKY13_02070"
     CDS             complement(1914..3746)
                     /gene="ftsH"
                     /locus_tag="PKY13_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT78738.1"
                     /translation="MAPRSRSDKLQAFVSHPVTVAVAAFLVMGAILLVSRLGTDPEEL
                     TLRKYREQVLDGRVRTAKLGEESNSVTGKLRNGDEYRTVIPHEYSDELTSLLIENDVD
                     VSADNETPSLAGSLLSDYLPLVIVLLVFGGFILNLQAGGKSAKFGQMRSKRNKDAPDV
                     SFADVAGADEAVEELGEIKEFLENPDRFAELGAKIPRGVLLVGPPGTGKTLLAKAVAG
                     EAGVPFFTIAGSDFVEMFVGVGAARVRDLFKQAKASAPAIIFVDEIDAVGRHRGAGVG
                     GGHDEREQTLNQLLVEMDGFATEAGVILMAATNRPDILDPALLRPGRFDRQIVVDAPD
                     LQGRKAILDVHARNKPLGAGVDLFVLARRTPGFTGADLANVINEAALMCGRRHGNTIE
                     MSDVNEAVDRVMAGPERKSRIMSDREKSITAYHEGGHALVGHLTPDADPIHKVSIVAR
                     GRALGWTLALPVEDRQSHSRNELLGRIAMLMGGRTAEELVFGEITTGAYDDIEQATRL
                     ARKMVTEFGMSDAIGPQRLTAATDEPYLGRDGTRAEMSNQVAAVVDAEIKRILDEAHE
                     RARVILTEHRSTLDLLAARLIERETLDEDQLGDVFAQVAGSSDE"
     gene            complement(3796..4863)
                     /locus_tag="PKY13_02075"
     CDS             complement(3796..4863)
                     /locus_tag="PKY13_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOT78739.1"
                     /translation="MSPALSELPPPTISFASDNAAAVHPAVMDAMVAANDGSALAYGA
                     DAWTDRVERRFAELFGTDVTVALCWGGTGANVVALQSLLRPWQAVICTDSAHIAVDEC
                     GAPERFTGAKLIDVAAPHAKLLPEQITEHLHLLGDEHHVQPAVVSITQSTEAGTLYTA
                     DEVAAICDTAHAAGLRVHMDGARIANATAALGGTVEALRATTVDAGVDVISFGGTKAG
                     MMYGEAVVFLDPSLGRDVKFVRKQAGQLPSKMRYVAAQFEALLADDLWISLARSANTR
                     AVELADALAGIDGVRLSRRPEVNSVFASLPRPAIDQLQAWSFFWDWDVHIDEVRWMTS
                     WATDADDIAAFVAGVRAVCPG"
     gene            4976..5818
                     /locus_tag="PKY13_02080"
     CDS             4976..5818
                     /locus_tag="PKY13_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78740.1"
                     /translation="MRQHRYIRLAAALTALSAAVAGTAVIGAAPAGAADECMNVTFPA
                     TRLIDWDGYNAKVEYSPTVSVALAAGSYGVTGSSSDGYPSRVGVTQTSEIWELQFLDA
                     TGTVIATSGLTGDLPDRVADATWSGSLGTVTLPAAATAVRAHHRPDAIADGSANSVVP
                     VAATVCPNGTPTTTTAPTTTAAPTTTAAPTTSAAPSSTAAPTTAPPTSATPTTAPIRA
                     TSIPTEVLAETTVVTTPATTPTGGALAVTGGDSKPLGGVALVLFGAGVILLGWRAQQI
                     SRRG"
     gene            complement(5837..6388)
                     /gene="hpt"
                     /locus_tag="PKY13_02085"
     CDS             complement(5837..6388)
                     /gene="hpt"
                     /locus_tag="PKY13_02085"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOT78741.1"
                     /translation="MSFADHDPDVGEVLLTADDIQKRIVEIGAELTAAYEGRIPLLVC
                     VLKGSYVFVADLARAIDLPVELDFIAVSSYGSSTRTSGVVRLVKDLDSDIAGRDVILI
                     EDIVDSGLTLRYLRRNLEARNPASLTVCTLIARASADIESLDVGYVGFTIADEDWLVG
                     YGLDVGQRYRNLGYLAKYVKPDS"
     gene            complement(6399..7319)
                     /gene="tilS"
                     /locus_tag="PKY13_02090"
     CDS             complement(6399..7319)
                     /gene="tilS"
                     /locus_tag="PKY13_02090"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT78742.1"
                     /translation="MTRDAAESPSSLTDRLLRRCTFPPAGSPVHVAVSGGRDSLALLV
                     LASAARLDVTAVHVDHGVRPGSADEAALVEGVARRFGAAFEAVRVVVDDGPNLEARAR
                     DARYRALPPDVLTGHTLDDQAETILLFLMRGTGPEGLAGIDRARRPLLSLRRAETGQL
                     CADLGLTVFDDPSNDDPRFRRNRVRAELLPLLDDIAGRDVAPLLARTADLQRSLLEVV
                     AAAAADVDPSDAREVASVSVGVASEALRAWWRRETREHYAPDADAVERMLGVARGDAT
                     AADIGSGWRIARTGQRLRLEQILGEVGQPR"
     gene            complement(7316..8395)
                     /locus_tag="PKY13_02095"
     CDS             complement(7316..8395)
                     /locus_tag="PKY13_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOT78743.1"
                     /translation="MATAVDWATAERVASRIARRGAPDLPSGYASVHRDFEELTAQAQ
                     TLVEAHTGWTSEAGLARARVVDRDDWIRANIASFQRMLRPLTDRLEEKLLPGPANFIA
                     SRVAGAEMGAVLGWMSTRVLGQYDLLVLEDEDVDDQDIVYYVGPNIAALEQRFDFPER
                     EFRLWVALHEVTHRTQFTGKPWLRSYFLEQVNGLMTMADPDPARFFAAIGRVAEAVRT
                     RRNPLDDGGIVTLFATAEQRHTMDRVGGLMSLLEGHGDVTMDRAGGNRIPSAGRFGDV
                     LRTRRSNASAPARLLQKLMGIEAKLAQYEQGEKFIEAVERVGGAPMLERAFAGPASLP
                     TLAEIREPARWVDRVGSGPLAVAGR"
     gene            complement(8407..8562)
                     /locus_tag="PKY13_02100"
     CDS             complement(8407..8562)
                     /locus_tag="PKY13_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78744.1"
                     /translation="MFALASLLAATESHVWHFWLAALLAPVTILAVLGTVASYFFKVT
                     RTRYPRK"
     gene            complement(8620..8859)
                     /gene="rsrA"
                     /locus_tag="PKY13_02105"
     CDS             complement(8620..8859)
                     /gene="rsrA"
                     /locus_tag="PKY13_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HOT78745.1"
                     /translation="MSDNCDVALAELYEYLDGELTEAARQRIEQHLRECSPCLEAFDF
                     EAEVRRIVADRCRDQCPEALRARIQSAIDGSGATA"
     gene            complement(8862..9497)
                     /locus_tag="PKY13_02110"
     CDS             complement(8862..9497)
                     /locus_tag="PKY13_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT78746.1"
                     /translation="MADPATFSRDAMVHMDALYSAALRMTRNAADAEDLVQETYLRAY
                     RGYERFEAGTNLRAWLYRILTNTFINSYRAKQRRPQESDLADVEDLYLYRRLGGVETS
                     TLGRSAEDQLMDLLSESEVKDAIEALPDKFRLPVLLADIEGFSYKEIAEIVDVPIGTV
                     MSRLHRGRRALQKSLWEFASARRLLPDVEATDVGGSPSSRTEVPETSPTGS"
     gene            9590..11002
                     /locus_tag="PKY13_02115"
     CDS             9590..11002
                     /locus_tag="PKY13_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT78747.1"
                     /translation="MPASYLLPDQPCRTVGEYRAAGQGDAVAAARALGAASLVELLES
                     SGLRARDHGAVSVGAKWAVLTGAGPDAGPRYLVANGTDAEPGSFTDRALLRSNPLGVI
                     EGVAVAAIAIGARDAFIAIRHSFETEYEILTTALAEAEVAGWLDRVSIKCVRTPEEYL
                     VGDERALLECVEGRAPLPVRRDPGIDGLFVGDGDRTDHAAPTRSSVTGRLSAPYNPTA
                     VESLETLVNVAAIAVNGASWFRSMGTPVSPGNILCTITGDVGRHGVIEAELGRRLYDV
                     LEEGGNGFLPTSPPKAVLSGVSSPVLTRSRLAAPLCWEGLNAVGANLGRAAFTVFGEP
                     TDMYAVAHRVASFLYVESCGLCPACKFGGGEVTAYLARIVAGTGDRRDLEALGGRLAT
                     VTDGARCDLAHRLRDVVSSIMWAFPGHAPSSNGSAAAPPGVLEHIVDIVDGVAAYGTV
                     QSRKRSDWVVEDRPVQLTRW"
     gene            11324..13015
                     /locus_tag="PKY13_02120"
     CDS             11324..13015
                     /locus_tag="PKY13_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT78748.1"
                     /translation="MGHDLVIRGGTVVDGTGAPSVRADVAIDGDRIVAVGDVDASGAA
                     EVIEADGRLVTPGFVDLHSHLDAQIGWDPLMSSSCWHGVTSVVMGNCGMTFAPLRPGQ
                     AETLANMMESVEDIPASAILQGLDWNWETYGQYLDAVDAMPKGINSAGHVGDVAVRTY
                     VCGEAACDEDFVASPEQLAEMARLVEEGLRAGAVGYSISRSLTHRVPDGRWVPGTWSD
                     PEEFFTIAEPLGRLGKGVLDCAPRYNETDGSTSRVDEEMAWIAELSRRTGRPFTFNLA
                     QMQSLGDHYRRVVDLSTQANETGAQLRPQTTPRSIGILFSLAANTLVDNLPSFAPLKQ
                     LDAAGRLAAIRDPEVRERLVAEAATQPGDAYGRMFLMEPDAGAVYRYGPDDSIAAQAA
                     AHGMSPIAWYLDVLDRTDGAAVANWPVLNQDFDAIEELMTSPVTVMGLADAGAHATQI
                     MDASQPTFFLSHWVRDRGLFGIEEGIRRVTSDTASLIGLTDRGVVRVGAFADLNVVDL
                     DGLRLPLPEIAHDFPGGSPRFVQRAEGIDHTVVNGRHFLDHGEHTGALAGRLLRG"
     gene            complement(13005..14006)
                     /locus_tag="PKY13_02125"
     CDS             complement(13005..14006)
                     /locus_tag="PKY13_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOT78749.1"
                     /translation="MIAVLDLAHHAASVAARADDERFHVPGWLWVAFIAGVVVMLLVD
                     LLVLNREAHEISVREAAITSAVWIAIGLSFSLVLWGVLDNGGTAAARYLTGYIVEKSL
                     SVDNVFVWAVIFGYFSVPSKYQHRVLFWGIFGALVMRAIFIFAGAALLDALDWMMYVF
                     GAVLLYTAVKVFRDDEDELDPEANPVLKLVRRFVPLSEHYEGQKLFTRHGAKLLATPL
                     FVVLVMIEITDVIFAVDSVPAILSISGDRFILFSSNALAILGLRALYFLLEAVRDRLV
                     YLPKGLGIILFYVGVKMIVSEWVHIDPFISLAVILGLLTITVVASLRATRTTADVSP"
     gene            14396..>14795
                     /locus_tag="PKY13_02130"
     CDS             14396..>14795
                     /locus_tag="PKY13_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78750.1"
                     /translation="MTDVETPPNHRTETVSRVLAAISTGTSDNELDREVLQVAFASEG
                     RSLLDLPEVGLWLALTEMALTEPHGPTGGADVVVIDLRDDAPAVPLLDDTALPGVPAS
                     ATARALAAIDEDVIDVDSVELDRGVVDRAEP"
     assembly_gap    14796..14895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14896..17360
                     /locus_tag="PKY13_02135"
     CDS             <14896..17360
                     /locus_tag="PKY13_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78751.1"
                     /translation="SVERPPVDPFGAVAFVPVLQAYVETALAGPLAIRLPDDARRGTA
                     TPDPWWPPALARSLAELDAYLRTVLAHPVAVPLRDPSVPGTAGVEPYWPPALARTLTS
                     LSAYLRAELDRPTSGRAPASRLAGATHGAEPDPYWPRHLVRAGTDELPVDDLADLPPA
                     PRNSEQLEHDLQLAIDAVALIGEADLDSFAAETQISLSRLVRREPLDRLGRVYPATLA
                     AFLVRQTRIHPSPTEFDALVPISALRRTNPLGLAFSEALVRLSKPVFREVEQRDEFDH
                     RVRYVRRMQLHVGITPDLVPAVLDAVITSHWAGARSGREVAQDWLSAHRAVSSMIGDP
                     TFRLLTRTDHGARMLEALLDLVRWRYSDPTPEAPPAALDPLPPALVAAAWGRLTAGAQ
                     PTDPWGERARPLLRLGRDPGSGPELELPRNASRWHLNGELLGYGSATHCKTVPLPTSR
                     SGKWAVTVGTTRRASPHDIGIQSAADDRIVLVFDESGRFHHHVLPLGGRTATVIAPSG
                     SRVDGAAERTLLVGRWIGFERIEVDLTDRTEISVSQRGRAQVTVAVDHRLGAHLLAAN
                     SDASQRLPRLVFRRHVPPTAEVELIVADGRTRHTSVLADLWIDDERGSFGLDHLVDPG
                     PPRTVTLTVTREGFEPAVHDLAVPGTPVSPDPAATVRTPPIAIESADVALRRLVGALA
                     SALSDAVAEDDDDDRDDPLAAHVWEAAITEDLERLAAWEQRHRRPLRQWRSTAATLAN
                     LDPLWAAHRSSERGRIVLPLLTAGLLAALDGAHRDLATRWSDEARHLAPHLGECVAAF
                     AAAYVEIRRNHPESRFRPIDLD"
     gene            17413..18606
                     /locus_tag="PKY13_02140"
     CDS             17413..18606
                     /locus_tag="PKY13_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOT78752.1"
                     /translation="MRLLHTSDWHLGRTFHGASLQREQAAVIDAIVDLTIAAQVDAVI
                     IAGDLFDRAVPPLEAVSLFDDAVARLRATGATVVAISGNHDSAPRVSVHDRLLSAGGV
                     TIRGDLRRAHEPVLVEPARGGSTVAIYPIPYLDPAAAAAVLLDDDERPTRRPGHDDVT
                     RAATARVRTDLGRRPGVRSVVVAHTFVVGGAPSDSERDLSMGNVEHVVIDAFDGFDYV
                     ALGHLHGPQAFADGRVAYSGSPLPYSFSEERHNKSVSIVELGPDGAATVDRVRLDVGM
                     NVRSITGRLDELVSDRSHTEAETAWVRATLTDTELPHQAMARLKRRFPRVVELRHRPE
                     GRVLDVSAAGPRRDRRDVSPLELTQRFWADQHGAAPTTRELELLEAAYRARLSTSGVP
                     EVGVS"
     gene            18603..21734
                     /locus_tag="PKY13_02145"
     CDS             18603..21734
                     /locus_tag="PKY13_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOT78753.1"
                     /translation="MRPHRLELAAFGTYPGHEVVDFDRLAEEGLFLIHGPTGAGKSTL
                     LDAITFALYGEFPGDRSRDRLVSHYVELGTQPLVEFEFTVSAGRHRISRTPAYTAPKR
                     GGGTTPKAGGVHLERLTDDGWVSVSTKQADVRAEIEHVIGLNVAQFHQVILLPQGKFE
                     RVLQADSDKREELLRTLFDTTLFSEMTSWLEERAAAAVSDAERIEARLDDLAAEARLK
                     WGDLEQVEGIDLRAPAEELVLAAVAAEGRLFDHDLGRPAETRHGDSAPSLHQLEPPEW
                     FRFVVERTDAFHAEAERTAAAGEGLLDEALVHQRTTDAVAARWDRRHAAQERVERLTA
                     RTESIDATRTVLLRSQLAEALRPSLDAVASAAEAASSAAAATATHQRTLRAAVESSPT
                     LPFELDAVVHDTLPDSAAIVTAGEAVGAHRRVLRGLSETAALVERHWAAAQRARRLAA
                     AATDESDAVTAELEELATAREAAATHLIEARSAAERVESLAAAAAIASERSEACRRLA
                     ALEPRHAAAREARIAAADTSATARLRLADRRENYLSGIAAVLAATLGPDDPCQVCGST
                     THPDPARPAHDQVERAEVDEAEAIADAAALALDRAEAEERELATEITLLLAKVGDRPT
                     LDDAVRLAADARAQVDAARRSAERIPALTARVEATIERSDVLEARRLELAGVIAGHHA
                     DADASVSQAEAAQLEISRVLGDGPDLDLRTTIASVEHVATTLSALREAVRAHDLATAA
                     LEQAEARLRSDLASSPFDDAAEATAALTSPDELARLAESVRHHDAELAAAAAVLATPD
                     LEALPDERPDTDGAHQAVSVARAAHTDAVQRAARLGAAAADLRRLDAEHHAASDRATA
                     ARAIAEETHRVALRCAGKLPPKVSLQRWILAAYLEEICEYANHRLAGMTGGRYRLLVD
                     REPARHAGKSGLGLRVSDAHTGDDRDVATLSGGETFQASLALALGVADTVAANAGGVE
                     LGALFVDEGFGSLDADALQLAMDELDELRAGGRLVGVISHVGALRERIGYGIEVTRTD
                     KGSHLEVCEVGPH"
     gene            complement(21778..22077)
                     /locus_tag="PKY13_02150"
     CDS             complement(21778..22077)
                     /locus_tag="PKY13_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78754.1"
                     /translation="MALRTAPIVAGSLIGGYLVARETKKRQLGGVVLAAGGVIAAREW
                     AARVGTAKMFALLAVYLLGFGASHPLAKKIGAWPAVFTAAGASAVAAHVVSDSAA"
     gene            complement(22102..22974)
                     /locus_tag="PKY13_02155"
     CDS             complement(22102..22974)
                     /locus_tag="PKY13_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT78755.1"
                     /translation="MHRPSRRRVAAVLTLAVLITGACLPAKPPPGSPTGACRPAPFSA
                     DVVAVLDAFGAASHHLTVAVYDDRSGCWYHLRPGQRMTTASVVKVEIMAGTLLRAQQQ
                     GRGPTLSEHQRLAAMIGRSDDAAASSLWTALGGEPGMERIGSTFGLGATDEIAPTWGL
                     TTTTAEDQARFLAALVHGPSPLDEASRTRAWGYLTDITPSQRWGVRAGVPPTWTVGHK
                     NGFAGSRCCGWRVNSVGYAADPAGGGYSIAVLSDGWRTLAEGIPLVEGASRAVAAVLT
                     SAPVVSESGAVPAG"
     gene            23026..24096
                     /locus_tag="PKY13_02160"
     CDS             23026..24096
                     /locus_tag="PKY13_02160"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOT78756.1"
                     /translation="MSGEVPVNIQPSGRFTTVRLPNHWYVACLASELGSTPIGRRVLD
                     VPLVLFRAADGSARALLDRCPHRNVPLSIGRCVGGEVECRYHGWRFDGDGTCVEVPGL
                     AEDRADRAVRRVPTFPVVEQDGMVWVAPSGVAPIVGPTRLAGVDEPGYRTIDLRLDVP
                     GPMLAAVENALDVPHTSFLHKGLFRGRRERLPIAVTIRHGVDSVEAIFEGEPVPPGLL
                     GKLLSIENGIVDHTDRFVVPALAQVEYRLEQHHIVLNAFYTPVDDDSCVLHAIAAFRL
                     PIPTTVARALVVPLARVVLNQDNRILRQQRDNIARFGGERFVNTPIDLLGPHILRLLR
                     RHERGETAPAEPAPDEHLTLLT"
     gene            24150..>24949
                     /locus_tag="PKY13_02165"
     CDS             24150..>24949
                     /locus_tag="PKY13_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT78757.1"
                     /translation="MNQIRRIAGTNEIDVRRVAVAGLSAGGVVVVAALALRTPGGQNI
                     DELARESRKLTDLGVRRAGVVVLRLSILTALPVTFLAALVSLGRHRRWRTEAACAVGT
                     IIAVTVAEVSKARLGRPALSDPRWLDERPTFPSGHATAGMAVALTAIAATEDRRSHVT
                     WIAAATGVWALTLTGSGWHRPSDIIAGFLIASTCFALVGLVRDPDPVLAIDDDERSSH
                     PGALRARTAARVGAAVLVSAYYLPLDAGPAGFRLHLPVYIAASILDVLV"
BASE COUNT         3753 a         9031 c         8427 g         3638 t
ORIGIN      
        1 caggccgcgt agatctccgt cctcgtcgac ccagggcgcg gcctcgccga agccagccgc
       61 agcgagtggg ccgacgagcg acgcgtgctg cacgccaccg atctcaacga ggaggtgccc
      121 gcccgggtgg agcagtcgtc cggctgccga cacgatccgg ccgaccaact cggttccgtc
      181 ggcgccaccg tcgagggcga gtcggggctc gtggcgctgg acgtccgccg gcaggaacgc
      241 gatcgcctcg gtcggaacgt acggggcgac ggccgtgatc aggtcgaacc tcggggagtc
      301 gcggatcggg gtgacgaggt cgccgacgac ggcgagcacc ccgttccgcc gagcgcaccc
      361 caccgcgcgt gggtcgatgt cgacgccgat cacggtcgcc gacggaacct cggccgcgga
      421 atgcgccgcg atcgcgcctg caccggtaca gacgtcgagc gccgcgccac cctcggggag
      481 aagcgctgcc gctcggcgtg ccaactcctc ggtctgaggt cggggcacgt agacgcccgg
      541 atcgatggtg taccaccggt cgcagaaccg gaaccggccc gtgatccact gcaatggctc
      601 gcccgtctcg cggcgggcaa gccatccgtc gagcgtggca tcgtcgggcg cgaccttgat
      661 cagctcggcg gcctcgtcct cggccgaaac acaaccggcg cgccggagcc gggcaacggc
      721 aacctcgtac gcgctcaggg cgagacgtac cctcggacca cggtccgacc gaggatgccg
      781 agggtcgcac gcagcgcccc ttcggagatg accgggaaga tgccggcgtc gcgccacacc
      841 tggtcgatct cggaccagtc gtagtagctg gtcacgaggg tcgcaccgtc ctcggccggt
      901 tcgaggcggt acccgtagat gtgatcgatc ggcggcttga tctggccgcg gatctgccag
      961 gcgatctcgc ggtccggttc gaacgtgtgg atctcgacgg tcacgtcgta gtgaccgagt
     1021 gggaagtcgt tgagcgcctc acggtccatg tggacgacga acgtgtcccc gacggccgat
     1081 acgggatcac cgtcggccga catcaacatg cccgagctgt cgatcgccac atggccctgc
     1141 gggtcacaca gcaccgcgaa gatggccgcg ggcggggcgg ggatggtgcg ctgcacctcg
     1201 atgcgctctt gggtcatggc gggcatcctt ccacgcgcca caccggttct gtagcgcgga
     1261 acgagggcgg ccgccccgaa tcgaggggct acccctgaat gaagcgcatc gcttcggcgc
     1321 gggccgacgg atcggtgcgg aagatcccgc gcaccgcgct ggtcacggtg gtcgagccgg
     1381 gcttgcggac tccacgcata cccatgcaga ggtgctcggc ctcgatgacc acgacgatgc
     1441 cacgcggttc gagcgccgac tccatggcct gggccacctg cgtggtgagt cgctcctgca
     1501 cctgcggtcg cttggcgtac ccatcgacga ggcgagcgag cttcgacagt ccggtgatac
     1561 ggccgttcgc cgaggggatg taggcgacgt gggcgtggcc gaagaacgga acgaggtggt
     1621 gctcgcacat cgaatagaag gggatctcac ggacgaggat catctcgtcg tggtcggcat
     1681 cgaaggtgac cttcaggtgc cgggccggat cctcgtgcag cccggcacac gtctccgcgt
     1741 acatgcgggc gacccgcgcc ggcgtgtcct tgagaccgtc acgttcggga tcttcgccga
     1801 tcgcagcgag gatctcccgc accgcagcgg cgattcgctc gacatcgacc ggcggtgccg
     1861 cctccgtcgt ggacggatcg atccggttga ggtcgtgcgc accctgccct gattcactca
     1921 tcggaggagc ctgccacctg ggcgaacaca tcgcccagct gatcctcgtc gagcgtctcc
     1981 cgctcgatga gacgggccgc cagcaggtcg agcgtcgagc ggtgctcggt cagaatgacg
     2041 cgggcccgtt cgtgggcttc gtcgaggatc cgcttgatct cggcgtcgac gaccgcagcg
     2101 acctggttcg acatctcggc acgggtgccg tcgcgcccca ggtacggctc gtcggtcgca
     2161 gcggtcagcc gctgtgggcc gatcgcgtcg ctcatgccga actcggtgac catcttgcgg
     2221 gccagtcggg tggcctgttc gatgtcgtca taggcaccgg tggtgatctc gccgaagacg
     2281 agctcttccg ccgtgcgccc gcccatcaac atcgcgatgc ggccgagcag ctcgttgcgg
     2341 gagtggctct gccggtcctc gacgggcagc gcgagggtcc agccgagggc gcgaccgcgg
     2401 gccacgatcg acaccttgtg gatcggatcg gcgtcggggg tgagatggcc gacgagtgcg
     2461 tgaccgccct cgtgatacgc cgtgatcgac ttctcgcggt cgctcatgat ccggctcttg
     2521 cgttcggggc cggccatgac ccggtcgacg gcttcgttca cgtccgacat ctcgatcgtg
     2581 ttgccgtgcc gccggccgca catgagcgcc gcctcgttga tgacgttggc caggtcggca
     2641 ccggtgaagc ccggggtgcg acgggccaac acgaacaggt cgaccccggc gccgagcggc
     2701 ttgttgcggg catgcacgtc gaggatcgcc ttgcggccct gcaggtcggg ggcgtcgacg
     2761 acgatctggc ggtcgaagcg gccgggacgc aacagcgcgg ggtcgaggat gtcgggccgg
     2821 ttggtggccg ccatgaggat gacaccggcc tcggtcgcaa agccgtccat ctcgacgagc
     2881 agctggttga gggtctgctc gcgttcgtcg tgaccgccgc cgacgcctgc gccgcggtgg
     2941 cgaccgacgg catcgatctc gtccacgaag atgatggcgg gggccgacgc cttcgcctgc
     3001 ttgaagaggt cgcgcacccg agcggcgccc acgccgacga acatctcgac gaagtcggag
     3061 cctgcgatcg tgaagaaggg aacgccggcc tcaccggcga cagccttggc cagcaacgtc
     3121 ttgccggtgc cgggcggccc gacgaggagc acaccccgcg ggatcttcgc gccgagttca
     3181 gcgaagcggt cggggttctc gaggaactcc ttgatctcac ccagttcttc gacggcctcg
     3241 tcggcgccgg ccacgtcggc gaacgagacg tcgggcgcgt ccttgttgcg cttcgaccgc
     3301 atctgcccga acttggcgga cttgccgccg gcctggaggt tgaggatgaa cccgccgaac
     3361 accaggagca cgatgacgag cggcaggtag tccgacagga gcgatccggc gagtgacggc
     3421 gtctcgttgt cggcgctgac gtcgacgtcg ttctcgatca gcagcgaggt gagctcatcg
     3481 ctgtactcgt gggggatcac ggtgcggtac tcgtcgccgt tgcggagttt gcccgtgacg
     3541 gagttcgact cctcgccgag cttcgcggtc cgcacgcggc cgtcgaggac ctgctcgcgg
     3601 tacttccgga gggtcagctc ctcgggatcg gtaccgagtc gggacacgag gaggatcgcc
     3661 cccatcacga ggaacgccgc cacggcaacc gtgacggggt ggctcacgaa cgcctgcaac
     3721 ttgtcggaac gagacctcgg tgccacggcg gtcaggctac cgacgaggtg tgctcacccg
     3781 gcacaggggg cggaatcacc cggggcagac ggcgcgaacc ccggcgacga acgcggcgat
     3841 gtcgtcggcg tcggtcgccc acgaggtcat ccagcgcacc tcgtcgatgt gcacgtccca
     3901 gtcccagaag aacgaccacg cctgcagctg atcgatggcg gggcggggca acgacgcgaa
     3961 caccgagttc acctccgggc gacgcgagag ccgcaccccg tcgatgccgg cgagtgcatc
     4021 ggccagctcg acggcgcggg tgttcgccga acgggccagt gagatccaca ggtcatcggc
     4081 gaggagcgcc tcgaactgcg ccgccacgta gcgcatcttc gacggcagct gtccggcctg
     4141 cttccgcacg aacttcacgt cgcgccccag cgaggggtcg aggaagacga ccgcttcgcc
     4201 gtacatcatg ccggccttcg tgcccccgaa gctgatgacg tcgaccccgg cgtcgaccgt
     4261 cgtggcgcgc aacgcctcga cggtcccgcc gagcgctgcc gtcgcgttgg cgatccgcgc
     4321 cccgtccatg tggacgcgca ggccggcagc gtgggccgtg tcgcagatcg ccgcgacctc
     4381 gtcggcggtg tagagcgtgc ccgcctcggt ggactgggtg atcgagacca cggccggctg
     4441 tacgtggtgc tcgtcaccga gcaggtggag gtgttcggtg atctgctccg gcagcagctt
     4501 ggcgtgcggc gccgcgacgt cgatcagttt cgcgccggtg aatcgttccg gcgccccgca
     4561 ctcgtcgacc gcgatgtgcg ccgagtcggt gcagatcacg gcctgccagg ggcggagcag
     4621 cgactggagc gcgacgacgt tcgcacccgt gcctccccag cagagggcga cggtgacgtc
     4681 ggtgccgaac agttcggcga accggcgctc gacccgatcg gtccacgcgt cggcgccata
     4741 ggccaacgcg ctgccgtcgt tggcggcaac catcgcgtcc atgaccgctg gatgcacggc
     4801 cgccgcgttg tcgctcgcga acgagatggt cgggggtggt agctcggaga gggccggaga
     4861 catggccgga ctgtagcggg agcgtccggc accccattcg agtgaactcc aagttacatg
     4921 cttggtattc gccccgaccg ggcgttagcc ttgcctcaga cgagggggtc tcacgatgcg
     4981 acagcaccgc tacatccggt tggccgcagc actcacggcg ctctccgctg ccgtggccgg
     5041 cacggccgtg atcggcgctg cccctgccgg cgccgccgac gagtgcatga acgtgacctt
     5101 tccggcgacc cggctcatcg actgggacgg ctacaacgcc aaagtcgagt acagccccac
     5161 cgtgtcggtc gcactcgctg ccggcagcta tggcgtgacg ggctcctcga gcgacggcta
     5221 tccgtcccgc gtcggcgtca cgcagacgtc ggagatctgg gaactgcagt tcctcgacgc
     5281 gacgggcacc gtcatcgcca cgtcgggtct caccggcgac ctccccgacc gtgtcgccga
     5341 tgccacctgg tcaggctcgc tcggcaccgt gaccctgccc gccgctgcca ccgcggtacg
     5401 cgcccaccac cgccccgacg ccatcgccga cggctcggcc aacagcgtcg tgcccgtcgc
     5461 tgcgacggtc tgcccgaacg gcacgcccac cacgacgacg gcgccgacca ccaccgccgc
     5521 cccgacgacg acggcagcac cgaccacgag cgccgccccg tcctccacgg cagcacccac
     5581 cacggcgccg cccacgagcg caacgccgac gacggcgccg atccgtgcga cgagcatccc
     5641 caccgaggtc ctcgccgaga ccaccgtcgt caccacaccc gccaccacac cgaccggcgg
     5701 agcgctggcg gtcaccggcg gtgactcgaa gccactcggc ggcgtggcgc tcgtgctctt
     5761 cggtgccggc gtcatcctgt tgggctggcg agcccagcag atcagccgac ggggctgacc
     5821 cgggcgcatt cgacgctcag gagtcgggtt tcacgtactt cgcgagatag ccgaggttgc
     5881 ggtagcgctg cccgacgtcg agcccgtatc cgacgagcca gtcctcgtcc gcgatcgtga
     5941 acccgacgta accgacgtcg agcgactcga tgtcggctga ggccctcgcg atgagggtgc
     6001 acaccgtcag gctcgcgggg ttgcgggcct cgaggttgcg gcgcagatag cgcagcgtca
     6061 ggccgctgtc gacgatgtcc tcgatgagga tcacgtcgcg acccgcgatg tcggagtcga
     6121 ggtccttgac gagccgaacg acgcccgacg tgcgcgtcga gctgccgtac gaggacacgg
     6181 cgatgaagtc gagctcgacg ggtaggtcga tggcccgggc caggtcggca acgaagacgt
     6241 acgatccctt gagcacacag accagcaacg ggatccggcc ctcgtaggcc gccgtgagct
     6301 ctgccccgat ctcgacgatc cgcttctgga tgtcgtcggc ggtgaggagc acctcaccga
     6361 catcggggtc gtgatcggcg aagctcatgc ggcgaaccct agcgaggttg cccgacctcc
     6421 cccaagatct gctccagccg gaggcgctgg ccggtgcggg cgatgcgcca tccacttccg
     6481 atgtcggccg cagtcgcgtc gccgcgggcc accccgagca tccgttcgac tgcgtccgca
     6541 tcgggtgcgt agtgctcgcg ggtttcgcgg cgccaccagg ctcgcaacgc ctccgacgcc
     6601 acgccgacgc tcaccgacgc gacctcacgg gcatcggacg ggtccacgtc ggccgccgcc
     6661 gcggccacga cctcgaggag cgaccgctgg aggtcggccg tacgtgccag gagcggtgcc
     6721 acgtcacgtc cggcgatgtc atcgagcaac gggaggagtt cggcccggac ccgattccgc
     6781 cggaatcgtg gatcgtcgtt cgacgggtcg tcgaacacgg tgagtccgag atcagcacac
     6841 agctgcccgg tctcggcccg acgcagggac aacaacggac ggcgagcccg gtcgatcccg
     6901 gcaagaccct ccggaccggt gccccgcatc aggaacagca ggatcgtctc ggcctggtcg
     6961 tcgagcgtgt gaccggtcag cacgtcgggc gggagcgccc ggtaacgggc gtctcgcgct
     7021 cgcgcctcga ggttgggacc atcgtccacc acgacccgga cggcctcgaa cgccgcgccg
     7081 aacctccggg cgaccccctc gaccagcgcc gcctcgtcgg cggagccggg acgaacgcca
     7141 tggtcgacgt ggacagcggt gacgtcgagc cgcgcggccg acgcgagcac caggagggca
     7201 agcgaatcgc gcccacccga caccgcgacg tggaccggcg accccgcagg agggaacgtg
     7261 caccggcgga ggagtcgatc ggtcagcgac gacggcgact ccgccgcgtc acgcgtcacc
     7321 gcccggcgac cgccaacgga cccgacccca cccggtcgac ccaacgggcc ggctcgcgga
     7381 tctcggcgag tgtcggcaac gatgcgggac cggcgaacgc ccgctcgagc atcggcgcgc
     7441 cgccgacccg ctcgaccgcc tcgatgaact tctcgccctg ctcgtactgg gcgagcttgg
     7501 cctcgatgcc catcagcttc tgcagcaacc gcgccggagc cgaggcgttg gaacgccggg
     7561 tgcgcagcac gtcgccgaac cggcccgcag acgggatccg attgccgccg gcacggtcca
     7621 tggtcacgtc accgtggcct tcgagcaggc tcatcagccc accgacacga tccatcgtgt
     7681 gacgctgctc ggccgtggcg aacagcgtga cgatgccgcc gtcgtcgagg gggttgcgcc
     7741 tggtccggac ggcctcggcg acccggccga tcgccgcgaa gaaccgcgcg gggtcggggt
     7801 cggccatcgt catcagaccg ttgacctgct cgaggaaata ggagcgcaac cacggcttgc
     7861 cggtgaactg ggtccggtgg gtcacctcgt gcaacgcgac ccagagccgg aactcccgct
     7921 ccgggaagtc gaagcgctgt tcgagtgccg cgatgttcgg cccgacgtag tacacgatgt
     7981 cctggtcgtc gacgtcctcg tcctcgagga ccaacaggtc gtactggccc agcacacgtg
     8041 tggacatcca gccgagcacg gcacccatct cggcgcccgc cactcgcgac gcgatgaagt
     8101 tggccgggcc gggcagcagc ttctcctcga gccgatcggt gagcggccgg agcatccgct
     8161 ggaaggacgc gatgttggcg cggatccaat cgtcgcgatc cacgacgcgg gctcgggcca
     8221 gacccgcctc cgacgtccaa cccgtgtggg cctcgaccag cgtctgcgcc tgagccgtca
     8281 gttcctcgaa gtcacggtgg accgatgcgt agcccgaggg gaggtcgggt gcgccgcgcc
     8341 gagcgatgcg gctcgctacc cgttcagccg tggcccaatc gacagcggtg gccacggatt
     8401 cgactgctac ttccgggggt accgggtgcg ggtcaccttg aagaagtagc tggcgaccgt
     8461 accgagcact gccaggatcg tcaccggggc gagcagcgct gccaaccaga agtgccagac
     8521 atggctctcg gtggccgcga ggagtgaggc gagcgcaaac attggtgtca gcttaggacg
     8581 cgacgagtcg catcgtgcaa cctggcgacc tgtccgtcgt catgccgtcg ccccggaccc
     8641 gtcgatggcc gactggatgc gcgcccgcaa cgcctcggga cactgatcgc ggcaccgatc
     8701 ggcgacgatg cggcgcacct cggcctcgaa gtcgaaggcc tcgaggcacg gtgagcactc
     8761 ccgcagatgc tgctcgatgc gctggcgggc cgcttcggtg agctctccgt cgaggtactc
     8821 gtagagctcg gcgagggcga catcgcaatt gtcggacatg gtcagctacc tgtgggcgag
     8881 gtttcgggaa cttcggtacg gctcgacggc gagccaccga cgtcggtggc ttcgacatcg
     8941 gggagcaatc gacgtgccga tgcgaactcc cacaacgact tctgcagcgc tcttcttccc
     9001 cgatggagac gactcatcac cgtaccgatc ggcacgtcga cgatctctgc gatctccttg
     9061 tacgaaaaac cctcgatgtc ggcgagcagg accggcaacc ggaacttgtc gggaagggct
     9121 tcgatcgcgt ccttcacctc gctctcgctg agcaggtcca tcagctggtc ctcggcactc
     9181 cgcccgagcg tgctcgtctc gacgccaccc agacggcggt agaggtagag gtcctcgaca
     9241 tcggcgaggt cggactcttg ggggcgacgc tgcttggccc ggtacgagtt gatgaacgtg
     9301 ttggtgagga tccggtagag ccaggcccgc agattggtgc cggcctcgaa gcgctcgtac
     9361 ccccggtacg cacgcaggta cgtctcctgc acgaggtcct cggcgtcggc ggcgttgcgc
     9421 gtcatgcgca gggccgcgct gtagagcgcg tccatgtgca ccatggcgtc acgtgagaag
     9481 gttgccgggt cggccatcgc gccagggtag gtcaccgccg ggaacaggga caggcccccc
     9541 ggtcgatcgg cgattccccg cggcggctcg ctctcgtagt gttgccgcag tgccggcctc
     9601 ctatctcctc cccgatcagc cctgccgcac cgtcggcgag taccgggcgg cgggccaagg
     9661 cgacgccgta gcggcggccc gggcgttggg tgccgcctca ctggtcgagc tgctcgagtc
     9721 cagcggtctc cgcgctcgcg accacggagc ggtctcggtc ggggcgaagt gggcggtgct
     9781 caccggtgcc gggcccgacg ccggcccccg ctacctcgtc gcgaacggga ccgacgccga
     9841 accgggcagc ttcaccgacc gtgcgctgct ccgctcgaat cccctcggcg tgatcgaagg
     9901 ggtcgccgtc gcggccatcg ccatcggcgc ccgcgacgcc ttcatcgcca tccgccactc
     9961 gttcgagacc gagtacgaga tcctcaccac ggcgctggcc gaggccgagg tcgccgggtg
    10021 gctcgaccgg gtcagcatca aatgcgtccg aacccccgag gagtacctcg tcggcgacga
    10081 gcgggccctg ctcgaatgcg tcgagggtcg cgctcccctc cccgtccgcc gtgaccccgg
    10141 gatcgacggc ttgttcgtcg gcgacggaga ccggaccgat cacgccgctc ccacccgatc
    10201 ctcggtcacc ggccgcctgt cggcgccgta caacccgacc gcggtcgagt cgctggagac
    10261 gctggtcaac gtggcggcca tcgccgtgaa cggcgcctcg tggttccgct ccatgggcac
    10321 cccggtgtcg ccgggcaaca tcctctgcac gatcaccggc gacgtcggcc ggcacggcgt
    10381 catcgaggcc gagctcggcc gacgtctcta cgacgtgctc gaggaaggcg gcaacgggtt
    10441 cctcccgacg tccccgccga aggccgtgct cagcggcgtc agttcaccgg tcctgacccg
    10501 ctcgcgcctc gcggcgccgc tctgctggga gggcctgaac gccgtcggtg cgaacctcgg
    10561 gagggcggcg ttcacggtgt tcggcgaacc caccgacatg tacgcggtcg cccaccgggt
    10621 tgcgtcgttc ctctacgtgg agtcctgcgg cctctgcccg gcctgcaagt tcggtggcgg
    10681 cgaggtgacc gcgtacctcg cccggatcgt ggccggcacc ggcgatcgtc gcgacctcga
    10741 agcgctcggc ggacggctcg cgaccgtcac cgatggcgcc cgttgcgatc tcgcgcaccg
    10801 tcttcgcgac gtcgtctcgt ccatcatgtg ggcgttcccc ggtcacgcgc cctcgtcgaa
    10861 cggctccgcg gccgcgcctc ccggtgtgct cgagcacatc gtcgacatcg tcgacggcgt
    10921 cgccgcctac ggcaccgtgc agtcgcgcaa acgaagcgac tgggtggtcg aggaccgacc
    10981 cgtccaactc acccgctggt gatcagcggt cggtcaggtg tgctggtcag gtgaactgat
    11041 cagcgtgtcg cgaaccgggc atcgtcgctc cggcggctgt tcgtcaactc agcggcctcg
    11101 cgacgcagcc agcgcttgca gtacgggcac ttcgccgccc gcccagcaca cagccccgag
    11161 cagtgacgac actcgtgttc cgagttcggc gttgcatctg ccatggatcc cccactcctc
    11221 gatggatcga tccattgaaa cgaattcggt gacgaaccga actgggccgt tggacccatt
    11281 ttcaccacca gcgatcgagc cgccacgggc gtagggtggc ccgatgggac acgatctggt
    11341 gatcaggggt ggcacagtgg tcgacggcac gggcgcgccg tccgtacggg cggacgtcgc
    11401 gatcgacggc gaccgcatcg tcgccgtggg cgacgtcgac gcgtcgggag ccgctgaggt
    11461 catcgaggcg gacgggcggc tggtcacgcc ggggttcgtc gacctccact cccatctcga
    11521 cgcccagatc ggttgggacc cgctcatgag ctcgtcgtgc tggcacggcg tcacgtcggt
    11581 ggtcatgggc aactgcggca tgacgttcgc gccgctccgt ccgggccagg ccgagacgct
    11641 cgccaacatg atggagtcgg tcgaggacat cccggcctcc gcgatcctgc aggggctcga
    11701 ctggaactgg gagacctacg gccagtacct cgatgccgtc gacgccatgc ccaagggcat
    11761 caacagcgcc ggccacgtcg gcgacgtagc cgtccggacc tacgtctgcg gcgaggccgc
    11821 ctgcgacgag gacttcgtcg catcgcccga gcagctcgcc gagatggcgc gcctcgtcga
    11881 ggaggggctc cgcgcgggcg cggtcggcta ttcgatctcg cgcagcctca cccaccgtgt
    11941 gcccgacggc cgttgggtgc ccggcacctg gtccgacccc gaggagttct tcacgatcgc
    12001 cgagccgctc ggccgactgg gaaagggcgt gctcgactgc gcgccccggt acaacgagac
    12061 cgacggttcc accagccgcg tcgacgagga gatggcgtgg atcgcggagc tcagccgtcg
    12121 tacgggccgg ccgttcacgt tcaacctggc gcagatgcag tcgctcggtg accactaccg
    12181 gcgagtcgtc gacctgtcga cacaggccaa cgagacggga gcacagctcc gtccccagac
    12241 gaccccgcgt tccatcggca tcctgttcag cctcgccgcc aacaccctcg tcgacaacct
    12301 gccgtcgttc gccccgctga agcagctcga cgccgcggga cggctcgccg ccatccgcga
    12361 ccccgaggtg cgcgagcgcc tcgtcgccga ggccgccacc caacccggcg acgcctacgg
    12421 ccgcatgttc ctgatggaac ccgacgccgg cgcggtctac cgctacggac cggacgactc
    12481 gatcgcggcg caggccgcgg cgcacgggat gtcaccgatc gcctggtacc tcgacgtgct
    12541 ggaccgcacc gacggcgcgg ccgtcgcgaa ctggccggtg ctcaatcagg acttcgacgc
    12601 gatcgaggaa ctgatgacat caccggtcac ggtgatgggc ctcgccgacg ccggcgcgca
    12661 cgccacccag atcatggatg ccagtcagcc gacattcttc ctgtcgcact gggtgcgcga
    12721 ccgcggcctg ttcggcatcg aggagggaat ccggcgggtc acgtcggaca cggcgtcact
    12781 catcgggctg accgaccgcg gagtcgtccg tgtgggggcg ttcgccgatc tcaacgtggt
    12841 cgacctcgac ggtctgcgcc tcccgctgcc cgagatcgcc cacgacttcc ccggcgggtc
    12901 gccgcgattc gtccagcgtg ccgagggcat cgaccacacc gtggtcaacg gacgccactt
    12961 cctcgaccac ggcgaacaca cgggcgcgct cgcgggtcgg ctgctcaggg gctgacgtcg
    13021 gcggtggtgc gggtcgcccg gagcgacgcg accacggtga tcgtgagcag cccgaggatc
    13081 acggcgagcg agatgaacgg gtcgatgtgg acccactccg agacgatcat cttgactccg
    13141 acgtagaaga ggatgatccc gagccccttc ggcaggtaca cgaggcggtc gcgaaccgct
    13201 tcgagcagga agtacagcgc ccgcaacccg aggatcgcca gcgcgttcga cgagaagagg
    13261 atgaaccggt cgccgctgat cgacaggatc gccggcaccg agtcgacggc gaagatcacg
    13321 tcggtgatct cgatcatcac gagcacgacg aacagcgggg tcgccaacaa cttcgccccg
    13381 tgtcgggtga acagcttctg gccctcgtag tgctcgctca gcggaacgaa ccggcggacc
    13441 agcttgagga cggggttggc ctcgggatcg agttcgtcct cgtcgtcacg gaacaccttg
    13501 acggcggtgt agaggagaac ggcgccgaac acgtacatca tccagtcgag ggcgtcgagc
    13561 agcgcggccc cggcgaagat gaagatcgcc cgcatgacca gggcgccgaa gatgccccag
    13621 aagagcaccc ggtgctggta cttgctgggc accgagaagt acccgaagat caccgcccag
    13681 acgaacacgt tgtcgaccga cagtgacttc tcgacgatgt agcccgtgag gtaacgggcg
    13741 gccgccgttc ccccgttgtc gagcacaccc cacagcacca gcgagaagct gagtccgatg
    13801 gcgatccaga cggccgacgt gatcgccgcc tcgcgaaccg agatctcgtg ggcctcgcga
    13861 ttcaggacga ggaggtcgac gaggagcatc acgaccacac ccgcgatgaa ggcgacccac
    13921 aaccagcccg gcacgtggaa gcgctcgtcg tcggcgcggg cggcgacgga cgcggcgtgg
    13981 tgggcgaggt cgagcactgc gatcacggcg tgagcctacg ggctgctcac ccccgccccg
    14041 gcgctccccg cgagccgaga acgcccatgg acccggccgg atcgtgcctg ttaggttccc
    14101 caccaagcct ggggaggtga tgaatcgcag tggcttgcct atgggctctt tccactgccg
    14161 cagtgccatt cgttaccgaa cgccgacgac ccgacgagcc cgtcgggaga tgacgcctgc
    14221 ctgccgcggc gtcgtcctcg cgggccttcc cgaccttcct caccgacgtg atgcctcttc
    14281 ccactccgcc tccccactcg caacgatccg accatcgccc gatggccggg gtcggcgcgt
    14341 tcggggtcgg cgcgttcggg atcggagcgg acggcgtcgc cgagccgtgg caaccatgac
    14401 cgacgtcgag acgccgccga accaccgcac cgagaccgtg tcccgcgtgc tcgcggccat
    14461 ctcgacgggg acgtccgaca acgaactcga ccgtgaggtc ctccaggttg cgttcgcctc
    14521 ggaaggccgg tcgctgctcg atcttcccga ggtcggcctg tggctggccc tgaccgagat
    14581 ggcgctgacc gaaccgcacg ggcccaccgg cggggccgac gtcgttgtga tcgacctgcg
    14641 cgacgacgcg ccggcggtac cgctcctcga cgacacagcg ctgcccggcg tgccggcctc
    14701 ggccacagca cgggcgctgg cggcgatcga cgaggacgtc atcgacgtcg attcggtcga
    14761 gctcgaccga ggggtggtgg accgcgccga acccgnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnccagc gtcgaacgcc cgccggtcga ccccttcggc gcagtcgcgt
    14941 tcgtcccggt gttgcaggcg tacgtcgaga cggcgctcgc cggcccgctc gccatccgcc
    15001 ttcccgacga cgctcggcgt ggcaccgcga cgcccgaccc gtggtggccg ccggccctcg
    15061 cccggtcgct cgccgagctc gacgcctacc ttcgaacagt cctcgcccac cccgtggcgg
    15121 tccccctccg ggatccgtcc gtgccgggaa ccgccggcgt cgagccctac tggccgccgg
    15181 cgctcgcgcg aacgctgacc tcactcagcg catacctccg agcagagctc gaccgcccga
    15241 cctccggccg cgccccggca tcccggctcg ccggtgccac ccacggagcc gagcccgacc
    15301 cctactggcc gagacacctc gtccgagccg gcaccgacga gctcccggtc gacgacctcg
    15361 ccgaccttcc gccggcaccg cggaactccg aacaactgga acacgacctc cagttggcca
    15421 tcgacgcggt ggcgctcatc ggcgaggccg acctcgactc gttcgcggcc gagacgcaga
    15481 tctcgctgtc gcggctcgtg cgtcgcgagc cactcgaccg actgggccgg gtctacccgg
    15541 cgaccttggc cgcattcctc gtccgccaga cccggatcca tccgagccct accgagttcg
    15601 acgccctcgt cccgatctcc gcgctccgac gcaccaaccc cctcggcctc gcgttctccg
    15661 aggcgctggt ccgcctgtcg aagccggtgt tccgcgaagt ggagcagcgc gacgagttcg
    15721 accaccgcgt ccgctacgtc cgcaggatgc agctccacgt cggcatcacc cccgacctcg
    15781 tgcccgccgt gctcgacgcc gtcatcacgt cgcactgggc aggtgcccgc agcggccgcg
    15841 aggtcgcaca ggactggttg tcagcgcacc gggcggtgag ctcgatgatc ggcgatccca
    15901 cgttccggct cctgacccgc accgaccacg gtgcccggat gctcgaggcg ttgctcgacc
    15961 tggttcggtg gcgatattcc gacccgacgc ccgaggctcc accagcggcg ctcgatcccc
    16021 ttccgcccgc cctcgtcgcc gccgcgtggg gccggctgac ggccggcgcc cagccgaccg
    16081 atccgtgggg tgagcgtgcc cgcccgctgc tccgactggg ccgcgacccc gggagtggac
    16141 ccgagctcga gttgccccgc aacgcgtcgc ggtggcatct gaacggcgag ctgctcgggt
    16201 acgggtcggc gacccactgc aagaccgtgc cgttgccgac cagccgcagc ggcaagtggg
    16261 ccgtcaccgt cggcaccacc cgtcgggcat cgccacacga catcggcatc cagtccgccg
    16321 ccgacgaccg gatcgtgctc gtcttcgacg aatcgggtcg cttccaccac cacgtcctgc
    16381 cgctcggcgg gcgcacggcg acggtgatcg ccccgtccgg ttcacgcgtc gacggtgcgg
    16441 ccgagcggac gctgctggtc ggccggtgga tcggcttcga gcgcatcgag gtcgacctga
    16501 cggatcgcac cgagatctcc gtgtcccaac gcggccgcgc ccaggtcacc gtggcggtcg
    16561 atcaccgtct gggagcgcac ctcctcgccg cgaacagcga cgcttcacaa cgcctgcctc
    16621 ggctggtctt ccgccgccac gtgccgccga ccgccgaggt cgagctgatc gtcgccgacg
    16681 gacgcacccg tcacacctcg gtgctcgccg acctgtggat cgacgacgag cgcggcagct
    16741 tcggcctcga ccacctcgtc gaccccgggc cgccccggac cgtcacgctc accgtcacgc
    16801 gggaggggtt cgagcccgcc gtgcacgacc tcgcggtccc gggcactccc gtgtcgcccg
    16861 accccgccgc gaccgttcgg accccaccca tcgccatcga gtcggccgac gtcgcacttc
    16921 gccgactcgt cggcgcgctg gcgagcgcac tgtccgatgc cgtagcggag gacgatgacg
    16981 acgaccggga cgacccgctc gcggcccacg tctgggaggc ggcgatcacc gaggacctcg
    17041 agcgcctcgc tgcatgggag cagcgccatc gtcgtcccct tcgacagtgg cggtccacgg
    17101 cggccacgtt ggcgaacctc gacccactct gggcggcgca ccgatcgtcg gagcggggtc
    17161 gcatcgtgct cccgctgctg accgccgggc tcctggccgc cctcgacggc gcccaccgcg
    17221 acctggccac gcggtggagc gacgaggccc gccacctggc cccccacctc ggcgagtgcg
    17281 tcgctgcgtt cgctgcggcc tatgtcgaga tccgccggaa ccaccccgag tcgcgattcc
    17341 gcccgatcga tctcgattga gcccgacggc agggttccgc tcactgtcac acccccgtcg
    17401 tagcttggtg ccatgaggtt gctgcacacc tccgactggc atctgggccg cacgttccac
    17461 ggagcgtcgc tgcaacgcga gcaggctgcc gtgatcgacg ccatcgtcga cctcaccatc
    17521 gccgcgcagg tcgacgcggt gatcatcgcc ggcgatctgt tcgaccgtgc cgtcccaccg
    17581 ctcgaggccg tgagtctctt cgacgacgcc gtcgcccgtc tgcgggccac cggtgcaacg
    17641 gtcgtggcga tcagcggcaa ccacgactcc gcgccgcgag tcagcgtcca cgaccggctc
    17701 ctgtccgccg gaggcgtcac catccgcggc gacctccgcc gggcccatga gccggtcctg
    17761 gtcgaaccgg cacggggtgg gtccaccgtc gccatctacc cgatccccta cctcgacccg
    17821 gccgcggccg ccgcagtgct gctcgacgac gacgaacgtc cgacccgccg cccgggccat
    17881 gacgacgtga cgcgggcggc gacggcgcgg gtccgcaccg atctcggtcg tcgacccggc
    17941 gtccggtcgg tcgtcgtcgc ccacacgttc gtggtcggcg gcgcgccgtc cgactccgag
    18001 cgcgacctga gcatgggcaa cgtcgaacac gttgtgatcg atgcgttcga cgggttcgac
    18061 tacgtcgccc tcgggcatct ccatgggccc caggcgttcg ccgacggccg cgtcgcgtac
    18121 agcggctcgc cgctgcccta ttcgttctcc gaggaacggc acaacaagag cgtgagcatc
    18181 gtcgagctcg gccccgacgg cgctgccacc gtcgaccggg tccgactcga cgtcggcatg
    18241 aacgtgcgat cgatcaccgg ccgcctcgac gagttggtgt cagatcgctc gcacaccgag
    18301 gcggagacgg catgggtgcg cgcaacgctg accgacaccg agctgcccca ccaggccatg
    18361 gcccgcctca agcgtcgctt cccgcgtgtg gtcgagctgc gtcatcgccc cgaaggtcgc
    18421 gtgctcgatg tctcggcggc cgggccccgg cgggaccgcc gcgacgtctc gccgctcgaa
    18481 ctgacgcagc gcttctgggc cgaccagcac ggtgccgctc cgacgacgcg tgagctcgaa
    18541 ctcctcgagg ccgcctaccg ggcgcgtctc tccacgtccg gggtgcccga ggtcggggtc
    18601 tcatgagacc acatcggctc gaactcgcgg cgttcggcac gtaccccggt cacgaggtcg
    18661 tcgacttcga ccgactggcc gaagagggcc tgttcctcat ccacggcccg accggcgccg
    18721 gcaagtcgac gctgctcgac gccatcacgt tcgccctcta cggcgagttt cccggcgatc
    18781 gcagtcgcga ccgtctcgtc tcgcactacg tcgaactcgg cacccagccg ctcgtcgagt
    18841 tcgagttcac cgtctcggcc ggtcgtcacc gcatctcacg cacgccggcg tacaccgcac
    18901 ccaagcgggg cggaggcacc acaccgaaag cgggtggggt acacctcgaa cgtctgaccg
    18961 acgacggttg ggtgagcgtc tccaccaagc aggccgacgt gcgcgccgag atcgagcacg
    19021 tcatcggtct gaacgtcgcg cagttccacc aggtgatcct gctgccgcag ggcaagttcg
    19081 agcgtgtcct ccaagccgat tcggacaagc gggaggagct gctgcggacg ctcttcgaca
    19141 ccacgctgtt ctccgagatg acgtcgtggc tcgaggagcg ggccgcagca gcggtgtccg
    19201 acgccgagcg catcgaggcg cgcctcgacg acctggccgc cgaagcccgg ctcaagtggg
    19261 gcgatctcga gcaggtcgag ggcatcgacc tgagagcacc ggcagaggag ctggtgttgg
    19321 ccgcggtggc cgccgagggc cggttgttcg accacgacct cggccgcccc gccgagacgc
    19381 gccacggcga ctccgccccg tcgctccacc agctcgaacc gcccgagtgg ttccggttcg
    19441 tcgtcgaacg caccgacgcg ttccacgccg aggcggagcg caccgccgca gccggcgaag
    19501 gtctgctcga cgaggcgctc gtccaccagc gaacgaccga tgccgtcgcc gctcggtggg
    19561 atcgccgcca cgcggcacag gagcgggtgg agcgtctcac agctcgcacc gagtcgatcg
    19621 acgcgacgcg caccgtgctc cttcgctcgc agctcgccga ggccctccgt cccagcctcg
    19681 acgcggtggc gtccgccgcc gaggccgcgt cgagcgctgc cgctgcgacc gccacgcacc
    19741 aacgcacact gcgcgctgcg gtcgagtcgt cgccgaccct tcccttcgag ctcgatgccg
    19801 tggtgcacga caccctcccc gacagcgccg cgatcgtgac ggccggcgaa gcggtcgggg
    19861 cacaccgccg ggtactccgg gggttgtcgg agacggccgc actggtcgag cgccactggg
    19921 ccgcggcgca gcgggcgcgt cgtctggcgg cggccgcgac cgacgaatcg gatgccgtta
    19981 ccgcagaact cgaggaactg gcgactgctc gggaggccgc cgcgacccac ttgatcgagg
    20041 cgcggagcgc tgccgagcgg gtcgagtcgc tcgccgctgc cgccgccatc gccagcgagc
    20101 gcagcgaggc gtgccgccgt ctcgctgcgc tcgagccccg ccacgcggct gcccgcgaag
    20161 cccgcatcgc ggcagccgac accagtgcca cggcgcggct tcggcttgcc gaccgccgcg
    20221 agaactacct gtcgggcatc gcggccgtac tggccgccac gctcggcccc gatgacccct
    20281 gccaggtctg cgggtcgacc acccatcccg atccggcccg gcccgcccac gaccaggtgg
    20341 agcgtgccga ggtcgacgag gccgaggcca tcgcggatgc cgccgcactc gcgctcgatc
    20401 gtgccgaggc cgaggaacga gagctggcga ccgagatcac tctcctgctc gcgaaggtcg
    20461 gtgatcgccc aacgctcgac gacgccgtcc gcctggcggc cgacgcccgg gcgcaggtcg
    20521 acgccgcccg ccgctcggcg gaacgcatcc cggccctgac ggcccgggtc gaggccacga
    20581 tcgagcgaag cgacgtcctc gaggcccgcc gactcgagct cgccggcgtc atcgccggcc
    20641 atcatgccga cgccgacgca tcggtgtcac aggccgaggc ggcccaactc gagatcagcc
    20701 gggtgctcgg cgacgggccc gacctcgacc tgcgcacgac gatcgcgtcg gtcgagcacg
    20761 tcgccaccac cttgtcggca ttgcgggagg ccgtgcgggc ccacgatctg gcaaccgcag
    20821 cgctcgagca ggccgaggcc cggttgcgca gcgatctcgc ctcgtccccc ttcgacgatg
    20881 ccgccgaggc gacggccgcg ctgacgtctc ccgacgagct cgcccgcctc gccgaatcgg
    20941 ttcggcacca tgacgccgag ctcgcagcgg ctgcggcggt cctcgcgacc cctgatctcg
    21001 aggcgctccc cgacgagcgg cccgacaccg acggggccca tcaggccgtg tcggtcgcgc
    21061 gggcagcaca caccgacgcc gtgcaacgag ctgcccgtct cggagccgcc gctgccgacc
    21121 tccgccgtct cgacgccgag catcacgcgg cgtccgaccg cgccactgcc gcccgtgcca
    21181 tcgccgagga gacccatcgg gtcgccttgc ggtgcgccgg caagttgccg cccaaggtgt
    21241 cgctccagcg atggatcctc gcggcctacc tcgaggagat ctgcgagtac gccaaccacc
    21301 ggctggccgg gatgaccggg gggcggtacc gcctcctcgt cgaccgcgag ccggctcggc
    21361 acgccgggaa gtccggcctc ggtctccgcg tcagcgacgc gcacacgggc gacgaccgcg
    21421 acgtcgcgac gctctcgggg ggtgagacct tccaggcctc gctggcactc gcactgggcg
    21481 tcgccgacac cgtcgcggcc aatgccggcg gcgtcgagct gggcgcgctg ttcgtcgatg
    21541 agggcttcgg ctcgctcgac gccgacgcgc tgcagttggc catggacgag ctcgacgagc
    21601 tgcgcgccgg cggtcggctc gtcggcgtca tcagccacgt cggcgccctc cgcgaacgga
    21661 tcggctacgg catcgaagtc acccgcaccg acaagggctc acatctggag gtctgcgagg
    21721 tcggcccgca ctagacgggc gagcctcgag ccggatcgcc gcgacgcgga gtgctcgtca
    21781 ggcggcgctg tcggacacga cgtgggccgc cacggccgaa gcgccggcgg cggtgaacac
    21841 tgccggccac gcgccgatct tcttcgccaa cgggtgtgag gcgccgaagc cgagtaggta
    21901 cacggcgagg agggcgaaca tcttggcggt gccgacacgg gccgcccact cccgggcggc
    21961 gatcacgccg ccggcggcga gcacgacccc gccgagctga cgcttcttgg tctcacgggc
    22021 gacgaggtag ccgccgatca gggagccggc gacgatggga gcggtgcgaa gagccatggg
    22081 cggcaactgt acggcgggcc gtcaccctgc cgggaccgcc cccgactccg agacgaccgg
    22141 ggccgaggtc aggacggctg cgacagctcg ggaagcgccc tcgacgagcg ggatgccctc
    22201 ggccaacgtg cgccagccat cggacaacac cgcgatcgag tacccgcctc cggcggggtc
    22261 tgcggcataa ccgaccgagt tcacccgcca tccgcagcag cgggatccgg cgaacccgtt
    22321 cttgtgaccc acggtccatg tgggcggcac gccggcgcgg acgccccacc gttgggacgg
    22381 cgtgatgtcg gtcaggtagc cccacgcccg tgtgcgcgac gcctcgtcga gcggcgaggg
    22441 gccgtgcacc agagctgcca ggaaccgtgc ctggtcctcc gccgttgtcg tcgtgagccc
    22501 ccacgtcggg gcgatctcgt cggtcgctcc cagaccgaac gtcgacccga tgcgttccat
    22561 ccccggctcc ccgccgagcg cggtccacaa cgagctcgct gcggcatcgt cggatcgacc
    22621 gatcatggcg gccagccgct ggtgctcgga caacgtgggc ccccgtccct gctgttgggc
    22681 ccgcagcagc gtgccggcca tgatctcgac cttcacgacc gatgccgttg tcatccgttg
    22741 ccccggccgc aggtggtacc agcagccgga ccggtcgtcg tacacggcga cggtgaggtg
    22801 atggctcgcg gccccgaagg catcgagcac cgcgacgaca tcggcggaga agggggcggg
    22861 tcggcacgct cccgtgggcg agccgggcgg cggcttcgcc ggtaggcatg cacccgtgat
    22921 caacaccgcg agggtgagga ccgccgccac gcggcgtcgc gacggacgat gcatggtggc
    22981 gagcctatgg atcttcgcgg gggtgggagc gctatcgtcg gcccgatgag cggcgaggtc
    23041 ccggtgaaca tccagcccag cggccggttc accacggtcc gcctgccgaa ccactggtat
    23101 gtcgcctgcc tcgcgagcga gctgggatcg acgccgatcg gccgccgtgt gctcgacgtc
    23161 ccgttggtgt tgttccgtgc ggccgacggc tcggcgcgcg ccctcctcga ccggtgtccg
    23221 catcgcaacg tcccgctgtc gatcggccgg tgcgtcggcg gcgaggtcga gtgccgctat
    23281 cacgggtggc ggttcgacgg cgacggaacc tgcgtcgagg tgcccggcct cgccgaggac
    23341 cgggccgacc gcgccgttcg ccgcgtgccg acgttccccg tcgtcgaaca ggacggcatg
    23401 gtgtgggttg ctcccagtgg cgtcgcaccc atcgtgggcc cgacacgtct ggccggtgtc
    23461 gacgagccgg gctaccggac gatcgacctc cgtctcgatg tgcccggtcc gatgctggcc
    23521 gcggtcgaga acgccctcga cgtgccgcac acctcgttcc tccacaaggg cctgttccgc
    23581 ggccgccggg aacggctccc catcgcggtc acgattcgcc acggcgtcga ttccgtcgag
    23641 gcgatcttcg agggcgagcc ggtgcctccc ggcctgctgg gcaagctgtt gtcgatcgag
    23701 aacggcatcg tcgatcacac ggaccggttc gtcgtcccgg cgctcgccca ggtggagtac
    23761 cgactcgagc agcaccacat cgtgctgaac gccttctaca ccccggtcga cgacgactcg
    23821 tgtgtgctgc acgcgatcgc agcgttccga ctgccgatcc cgaccacggt cgcccgcgcc
    23881 ctcgtcgtgc cgctggcgcg ggtggtgctc aaccaggaca accggatcct gcgacagcag
    23941 cgtgacaaca tcgcccggtt cggcggtgag cgcttcgtga acacccccat cgacctgctg
    24001 gggcctcaca tcctccgact gctccggcgc cacgaacgcg gcgagaccgc gccggccgag
    24061 ccggcgcccg acgaacacct gacgctcctg acctgaggcg acagggtccg tacgacctca
    24121 gcgaccaacc acgggcctac cgttgtgtcg tgaatcagat ccgacggatc gccggcacca
    24181 acgagatcga cgtgcgccga gtagcggtcg ccggtctcag tgcagggggc gtggtggtgg
    24241 tggctgcact ggccctccgc acgccgggag ggcagaacat cgacgaactg gcacgtgaaa
    24301 gtcgcaagct cactgacctc ggggtccgcc gggccggagt ggtggtcctt cgactgagca
    24361 tcctcaccgc actccctgtc acgttccttg cagcactggt gagtctgggg cgtcatcgcc
    24421 gctggcgcac cgaagcagcg tgcgctgtcg gcacgatcat cgccgtcacc gtcgccgagg
    24481 tgtcgaaggc gcggctcgga cgcccagcgc tctccgaccc gcgatggctc gatgaacggc
    24541 cgaccttccc gagcggccac gccaccgccg gcatggctgt tgccctcacg gccatcgccg
    24601 ccactgaaga ccgacgctca cacgtcacat ggatcgccgc agccaccggt gtgtgggccc
    24661 tcacgttgac ggggtcgggg tggcaccgcc cgagcgacat catcgctggg ttcctcatcg
    24721 cctccacatg cttcgctctg gtgggactcg tgcgcgaccc cgatcccgtt ctcgccatcg
    24781 acgacgacga acgttcgagc caccccggag cactccgcgc gcgcacggcg gcgcgtgtgg
    24841 gtgccgccgt gctggtgtcg gcgtactacc ttcccttgga tgccggccct gcggggttcc
    24901 ggttgcacct tccggtgtac atcgccgctt cgatcctgga tgtccttgt
//
LOCUS       DAOSOA010000014        23147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_2235_length_23147_cov_6.810021, whole genome shotgun sequence.
ACCESSION   DAOSOA010000014 DAOSOA010000000
VERSION     DAOSOA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 23147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23147
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_23147_cov_6.810021"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1090
                     /gene="map"
                     /locus_tag="PKY13_02170"
     CDS             161..1090
                     /gene="map"
                     /locus_tag="PKY13_02170"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT78758.1"
                     /translation="MARRKHRTALLDPPPSAPVKPGMVSPRRTVPAGIARPEYALTGE
                     PSSRASRAVRTPEEIEHMRIAGRVAAEVLIEVGTAVAAGVTTDELDRIGHDACIARGA
                     YPSPLNYRGFPKSLCTSVNEVICHGIPDSRKLLEGDVVNVDVTAFIEGVHGDTNATFV
                     VGEVDHASRDLIRITRQAMHAGIATVRPGSRINEIGRAIEAAVKGGNYGVVREFIGHG
                     IGDQFHTSLQIPHYYQASARTVIEEGMTFTIEPMITIGSPALYVWDDDWTAVTLSGQR
                     CAQFEHTLLVTSDGVEILTLPDGTPPAEELFAP"
     gene            1246..1848
                     /locus_tag="PKY13_02175"
     CDS             1246..1848
                     /locus_tag="PKY13_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78759.1"
                     /translation="MRTPLRVRLRSPAAYWSLVAVLAVGTIVWIERAERDLARRATAL
                     GPTTQVAVVVGDHAGGEQLDDADVELRDVPRSAVPADALTAAPHDRRLRTDVRAGEVL
                     TSGRFAAGGRSEIAAQIPPGRQAIAVPRTSAALDLRPGDQVALLGLGSSGASEVIVDD
                     GLVVAATEGATTIAVPLGDVGDVADAVLAGTMTVVLSGSG"
     gene            complement(1878..3230)
                     /locus_tag="PKY13_02180"
     CDS             complement(1878..3230)
                     /locus_tag="PKY13_02180"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT78760.1"
                     /translation="MTETGIDLRQLASRVAGQAASGEQIDVMVGHGRSTSVKVFGGEV
                     ESFTSAESYAVGIRVIAGGRQGFASAGSIDPDVVAETLAEARDNVTFGEVDEFYGLAE
                     PDGIAATEHDHWNEAILGIDAQTKIDLALRLEADVRARDPRISGVRVAAWGDSAGEVA
                     YAASNGISVYDRGTSCSIGIQALANDNGETQTGAAGDAARSLEALDVERVIADAADRA
                     TRLLGATKPPSGKISILLEPRLAMTLLGIVADMVDGESVFKGRSPFADRVGEQIASPL
                     LTFVDDPTDSRSIAATAYDGEGLACRRNVLIDAGNLGAFLHNSYTGRRAGTASTGSAL
                     RGSRSLPGVGAQVLVMEPGARSFDELVASVDHGLYVSGFSGLHSGVNPVSGDFSVGAD
                     GLMIRNGAIAEPVREMTLASTIQRLLLDIVEVGGDLEWLSSGDAAASLIIADVSMSGA
                     "
     gene            complement(3235..4584)
                     /locus_tag="PKY13_02185"
     CDS             complement(3235..4584)
                     /locus_tag="PKY13_02185"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT78761.1"
                     /translation="MSPDELMEVAERVLAMAAPEEQVEVVAAHSRDTEVRAYDGEIEA
                     FTSAESAGFGVRVIRGHRQGFAYAGTLDADVVAETLGEARDNAAFSTVDEFTGLVEPD
                     GVAEVTLHLYDEALAGLGPDRKIELALELERLVCAGDPRILGVEMAEYADSIETAVIA
                     STTGIRSISRETGCSLAAYSLAGVDDDVTTGFGFSVGRGLDDLDLEDTARQAVERAVR
                     MLGAGRAPSRRLTVVFDPWVTAQFLSIVGESFSGSEVLKGRSFLADRVGASIAPPTVT
                     LVDDPTDGRWPSATPSDAEGLATRRNVLIDAGVVSGFVHDGYSARALGARSTGSAVRH
                     GFKSTPGAGTQSISLLAGDHDPASILAAVGDGIYISDVQGLHSGVNPVSGDFSTGIEG
                     MVIRGGELAEAVKEVTIASTLQRMMADISMVGSDLTAMPLDATGVTLAIADVTLSGS"
     gene            complement(4683..5684)
                     /locus_tag="PKY13_02190"
     CDS             complement(4683..5684)
                     /locus_tag="PKY13_02190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT78762.1"
                     /translation="MSVPLSILDLAIVGAGETVRDSLEGSVALARAAERAGYRRVWYA
                     EHHNMASIASSATSVLIAHVAAHTESIRLGSGGVMLPNHSPLVIAEQFGTLESLHPGR
                     IDLGLGRAPGTDQNAMRAMRRNGESSDRFPQDVRELQAYLSDERQVDGVTATPGRGTK
                     VPLYILGSSLFGAQLAAAFGLPFGFASHFAPTALSQAVALYRSEFRPSEQLDRPHVIA
                     AVNAIVADDEADAQQQFQVTKRRRVALLLPSGRSYSDDEADRILDSPQGRQLQQMAQY
                     SGVGTPTQVTEYLDRFAEHADADELIVAHQSPTVEERIRSVELLAEAAELGTAAGRR"
     gene            5978..7813
                     /locus_tag="PKY13_02195"
     CDS             5978..7813
                     /locus_tag="PKY13_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT78763.1"
                     /translation="MPAASISTVLSPLQGTVVEVRVAAGERVAPGATLVIIESMKMEH
                     LVETPGAGVVERILVSAGDMVTADQALVDLVLTPDDEPAPSAAATTADEHSIERADLA
                     AVTARHATGLDAHRPESVQRRRQRNQRTARENVDDLVDEGTFVEYGPLAIASQRGRRP
                     LEELIERTPADGLVGGVGEIEGRPAVVLSYDNTVLAGTQGHQNHRKKDRLFEVAERQR
                     LPVVLFAEGGGGRPGDTDVPGVSGLDVMAFDLFARLSGWVPLIGIAAGRCFAGNAALL
                     GCCDVIIATLDSNIGMGGPAMIEGGGLGVVAADDIGPVGVQAPNGVIDIVVADEAAAV
                     AAAKQYLSYFTTSVTPDHSAVDQSVLRDRVPENRLRVYDVRQVIEAIADADSVLELRT
                     GFGTGIVTTLARVEGRSLGIVANNPSHLGGAIDADGADKASRFLQLCDAFDLPVVFLC
                     DTPGIMVGPAAEETALVRHVNRLFVTGANLSVPTGTIILRKGYGLGAMAMCGGSFKSP
                     LFCVAWPTGEVGGMGLEGAIKLGFRRELDAVADPTERAAMYDSLVAAAYERGRALNVA
                     SHFEIDDVIDPADSRRWITTLFTSAASDLNTRHRLPKKRPYIDTW"
     gene            7881..8753
                     /locus_tag="PKY13_02200"
     CDS             7881..8753
                     /locus_tag="PKY13_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOT78764.1"
                     /translation="MTRQRLLAAIVPLVFLASACQTEQVMWGACTAGNDPWGTDGRYV
                     LACQNGEWIPVMTVAEFVAIKQGKQVKIAPLPTRPTTTTTTTTTTTAAPTTSTTSSTT
                     SSTTSTTSTTSTTSTTVPMASPSISFVNPDEGPTAGGQATVITGTGFTGTTAVTIAGT
                     SASFTIDSDTQITATTPAHAAGAVAVAVANPSGTDTFNGGYTYVSMPTISGLSVSTGP
                     VSGGTTVIITGTNLSGVGSVDFGPTAAASFTVTSATQITAVSPAGAAGAVNVRVTSWG
                     GTSDVSLADEFTYV"
     gene            complement(8794..10086)
                     /locus_tag="PKY13_02205"
     CDS             complement(8794..10086)
                     /locus_tag="PKY13_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOT78765.1"
                     /translation="MADPFVQLPAVEVRYLDRSVGAVAVDPSRGVPVFEYFPDWVDSG
                     ENLSPIALPRLRGPRLFPELANTSFRGVPGLVADSLPGTFADLLTTAWLARHGIQPGQ
                     VSVVDRLGYIGARGVGAVTFHPSVSDDLASTASVVDLAEAVDTARSALRGTLQADDAG
                     STLEQVLDTSGSAGGARAKAAVAIGPDGEVRSGQHDAPAGFTHWLLKFDVSPGQRIGQ
                     TTGYGQLEYAYHLMAVDAGLEMAECRLLEVDGLVHFLTRRFDRPTPSAKLHVQSLAAL
                     AHLPPEHPGAHSYEQFMQTCGRLELGVDDRRRAFRHVVFNIAAAVRDDHTKNFAFIYE
                     NQQWRLAPAYDLTFAFFDGGGWLAHHQLRISGSVDGADRPTLADLAERAKVPEPDDVI
                     DDVIAAVGRWREHAAAAGVLVDHADVVASELGRTALAG"
     gene            complement(10525..11916)
                     /locus_tag="PKY13_02210"
     CDS             complement(10525..11916)
                     /locus_tag="PKY13_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT78766.1"
                     /translation="MIDESVLERVLGAATKGGASFAEVFAEDKRSSSAVLDDGKVEEL
                     TSGRDRGAGIRVVVGESTGFAHTADLSEASLIATAEIAAAAARGSGSGTTVVDLSKTS
                     APRPNVVQLLPGDVPKARKVELLTRANEVARSQGSAITQVMVRYSDSRRRILVANTDG
                     TFADDDQVKTVFSISCVATGDTGMQTGRESVGHTVGFELFDQVDVEELARNAANRAIT
                     KLAARPAPSGQMTVVIGSGGGGVMFHEACGHGLEADLVAKGASVFANRIGEQVASPLV
                     TLVDDGTMGGEWGCFAIDDEGRAAQRNVLIQDGILVDYMWDGLRARKEGRASSGNGRR
                     QSYQHLPMVRMTNTYLLGGEGDKDSIVADTESGVYIAHLGGGQVNTATGDFVFGMTEA
                     YLIENGEITEPLREGNLIGNGPEVLLNIDAVADDFAMGPPGTCGKDGQGVPVGDGVPT
                     LRVHGLTIGGTAA"
     gene            12006..12380
                     /locus_tag="PKY13_02215"
     CDS             12006..12380
                     /locus_tag="PKY13_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOT78767.1"
                     /translation="MLDTGLEATVELVVADSDTAQAMGSGDVATLATPRMIALCEQAT
                     MSALDGHVDAGFTTVGLSVQVDHLRPTAVGGKIWAEAHLDKVEGRRLCFSVAAKDERG
                     LIGAGRVTRVVVEVEHFMEKTR"
     gene            12439..12774
                     /locus_tag="PKY13_02220"
     CDS             12439..12774
                     /locus_tag="PKY13_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78768.1"
                     /translation="MGSQKRATSITGLGLISVGAAFTLVGGLGVGFRAAGLGYLGQSL
                     VALTLGLGMLWVGLRVAAAARRAIFESVRTTPMRAHTVYTTAAPQPARVRVSAWTSPQ
                     LALPPASSR"
     gene            12819..13529
                     /locus_tag="PKY13_02225"
     CDS             12819..13529
                     /locus_tag="PKY13_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78769.1"
                     /translation="MPILSARGVRKIYRTGAVEFEALRGIDIDLGAGEMVTVMGPSGN
                     GKTTLMNCLSGLDDIDAGTVVIDGEDISAMSDARRTDHRAGRMGFIFQSFNLIGVLSA
                     VENVELPLLLGGSKPKAARSRAHDMLTRVGLDGRDKHRPAELSGGEQQRVAIARALVS
                     EPAIIWADEPTGNLDSTTAGAVLDLLLEVHSSGQTLVIITHDADIGASGERLIEVRNG
                     QIEYDGSPDHAHHVHAPR"
     gene            13540..16452
                     /locus_tag="PKY13_02230"
     CDS             13540..16452
                     /locus_tag="PKY13_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT78770.1"
                     /translation="MNPVWIVVGIAVLGLLITAVDLAASPTYRRLAVRSISRRRGEAM
                     LIVVGAMFGTAIIGAALIVGDSFDGSIRDIARTELGPIDVRAQIHPDDDVRREVLQLE
                     DRVAHSGIDHVDGFLPVVDAAAVLDNGRRGAGQRIDPTNCLLEMDFTQARRFGPDPIS
                     GIEAAGDTPAEGEAVMNRDVADVLGITAGDDLTVHLYGRSETLVVRTIVPTIGLAGYC
                     GAIVAPGTIESMWTAGGGATTAGTARGPTGELFVSLEGGVFDSTRYSERAERQIRAAV
                     KAGGFTDANVDPVKANRLESAERNGSQLRTIFSGIGGFSVISGILLLVNLVIMLAEER
                     KVEFGLLRAVGFKRAALLRSFSLEGSLYALSAAVLGAIVGVGVGALVIEGTQKIFAEP
                     NSTFRIDLFVHARTLLLAAGLGYAISIVTIWLASARVSRLNIISSIRDLPAPRRSGRH
                     LGRISLAGLGVVAGSIATVVGVRAGSQIPLMAGIPVASFSAIGVVTQMNFHSQRPVRV
                     ASVVGPIAAIAWTLGVFTLFSTQMEHAGIAVFVVMGLLLVAAAVIIAVAFAPLWGHAA
                     AARGGSFALSCRLGFAYPLARSVRTGLILAMFSLVIFTMVFMASLAATIDGQASSAAG
                     DLDAGYDIVVDTNPSNPVSETDLGGRPGVAAVTTFTQSGPLFTNRYQREPTRWLVSGF
                     DEGMLRFRPPALSKRLDRFADDRSVFDAVLDDPTLIVVDDQFLLRGGGPKDDGPEPGD
                     RVEMTNPAGDRRTVTVAGVLKTDAIFQGSLWPKTAVAEFMAPATSETRAFLAAAPGST
                     PSRLAAIARDLQAATVTQGTDVSTFTQIVADELGRTTSFMRLLEVYLGFGLLIGIAGL
                     GVVMVRAARERRHEIGTLRAMGLQGRVVSRAFLIEAFFIAGGGVIIGTSLALVTAYQV
                     VVNSSAFSIAAGSFVVPWVPLVLIAIVPTGFALLAAGQPARAVGRIRPAVALRIAD"
     gene            complement(16464..17306)
                     /locus_tag="PKY13_02235"
     CDS             complement(16464..17306)
                     /locus_tag="PKY13_02235"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT78771.1"
                     /translation="MDTTSHDLGAVRLHALEAGVGGAPLLLVHGFTGCKEDFAEEVDG
                     LAELGYHVVAPDLRGHGESDQPDDEASYGLRSFADDLFALTERLGWASFDLLGHSMGG
                     MIAQVMVLEQPDRVERLVLMDTHHGVVGDLDLDLVAIGIELARTQGLEVIQQILQMSD
                     VENPAYERACAARPGYREWSENKMLKCSAAMYAAMLHEMSTIDDRLDALGSVRVPTLV
                     QVGELDAAFVGASERMAATIPGAQLVVHPDGAHCPQFESTESWRSSLHEFLGAASSRA
                     TAVA"
     gene            17323..18459
                     /locus_tag="PKY13_02240"
     CDS             17323..18459
                     /locus_tag="PKY13_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT78772.1"
                     /translation="MPVGSAPTAAAWNTGAIEPDPPLLGVSTVDLAAELAGEDDGHPP
                     RRRSPVLTLVVRVLLSAVMLAVLVWRLPEVRWSDLGPRWRSTSAVWLVAAGLTMSAAF
                     ALSTLRWRRVLVAMRPAPRYRRMLSHFLAGQFVSNVLPTAFGGDVIRVSRLGRDIGDH
                     PTAFASVTIERMTGWLVLPLISGVALLAQPDLLSLGSATALAVAIDGGTIVALVVVLL
                     VASNRRFGASVETVTGWRRFFVAIHLGVDALRRDRRGSLEVIAAGAAFQTLQCLAVWM
                     AAEAIGLQGQVTVGVALAFFPAAAIAQNLPVGLGGLGVREGIFVVFFGAVEAPRALSI
                     TLGLVVYGLTIVTSSLGAPSFVIGSRTGSQNPHTGDVTPPLGGS"
     gene            18499..20346
                     /gene="dxs"
                     /locus_tag="PKY13_02245"
     CDS             18499..20346
                     /gene="dxs"
                     /locus_tag="PKY13_02245"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOT78773.1"
                     /translation="MLLDNIDGPADLRRLDYPALDQLADEIRTVVVDAATRAKGHLGS
                     NLGAVELTLALHRVFDSPNDIILWDTGHQAYVHKLVTGRRDAFDSLRQPGGLSGYPSR
                     TESSHDWIENSHASTVLSYAHGLATAQHLAGDPKRQIVAIIGDGSMTGGMAYEGLNNL
                     GHSGRNVIIVLNDNGRSYAPTVSRLGESLSRLRSNPMYRRQQARLEKVLGDLPMVGGY
                     LERGVDGAKAAVREILEPPAFFENLGVKYVGPFDGHDIAGLERALRNAQAIDGPVVVH
                     VLTQKGRGYAPAENDQVKAMHDMGEVKDGSYTAAFSEAMLKEGERRPEVVAITAAMPD
                     STGLLPFGERFPGRLIDVGIAEQHAVTSAAGMAMGGLRPVFAVYSTFLTRAIDQVNLD
                     VGLHHQPVIFALDRAGITGDDGASHHGVLDMVLLTKVPDMTVFAPSSYGEVGQMLSDA
                     LDLCTGPAAIRFPKTMPPTTEPGDTGSGLTGRKVRDGDEICIIGVGKMLEVAREAADR
                     MAESGTSVTVWDPRVVKPLDADMLADAARHRLVVTIEDGLRDGGVGSAIADALRDLAP
                     VQGPSVRVLGVPSAYLQHDKPDQLLARLGLNADGVVAEISAWQRASQAH"
     gene            complement(20421..21152)
                     /locus_tag="PKY13_02250"
     CDS             complement(20421..21152)
                     /locus_tag="PKY13_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOT78774.1"
                     /translation="MSADTETIGSGSAPAEPVASSGAAGSVVSPFAAGSVVSPFANDL
                     PVIEITEAALETVLSIRAGEDNPDELGLRIEITGSRGSEYTYDLSFDEISNAVADDQL
                     IEASGVTVIVPAETVEKLRGSVLDLPRTAGQGGLVIRNPNRPDPLAGIDLDLEGTIAE
                     QVEQLLEQAINPSLAAHGGFASLVGVDESNNVYVKMGGGCQGCSASALTLAEGIKRQI
                     KDLVPDVLDVIDATDHQAGENPFYK"
     gene            complement(21279..>23147)
                     /locus_tag="PKY13_02255"
     CDS             complement(21279..>23147)
                     /locus_tag="PKY13_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78775.1"
                     /translation="RRPMGDIRMTSSRRPRLGAAAVGALLTVSLVGCVGPDPLFGGVA
                     APPRSEPSTCGAPSFSCALPFPSDRWQIPDASTATGIRLEVPPNSISRDVLDQLGPRD
                     ETQEALIGADGFSPLTPIVFQLPDALGVGSVPADGGELVRVVDATTGERVPVRAEVSS
                     YLADERGSHNLVLVWPAVKFAYGHQIFAALRRGVTALDGTPAHASPSLEQASPATRAV
                     AARVEPSIPWDDYLTATSFVVRTEQSIVADVDRLAGLVRADDHPIRNVEVRPSLIGGA
                     AALSGQVRITDFRDAAGVISRDGTAVGHELWVDFMAVLPERSAATGGAPVIMYGHGIT
                     VMKESMLVVAGQNAAKGYATIGIDVPNHGSRINEGGHIIDLAYPSLLGRVESLLLQGE
                     LDQLSLLLAIKVHLGSLDVLPRNWLAGTGGDGVADLDVGHVLYEGTSLGGVLGATFLA
                     LAPEVEGAFLQVPGSGIIDTLFHSLVWEMFRGIVPTGAPYGETHVLTFFAQNLLDRAD
                     NTYYLDRIRQRGTPIFLSYAVDDGIVPNTSTERMISLLDLPLVGRQAGPIPSWLASSR
                     SSTMPTDGRGYGQVPTGELYGNILKPFLTHLEFMNPISVSALDAWLDGRTAALLRG"
BASE COUNT         3581 a         8174 c         7878 g         3514 t
ORIGIN      
        1 gtcgagcgcg tccacctcga cgaccccgtc gagcgactca ggatcctcga cgtcgtccga
       61 ctcgggcagt tcctcgtcga gcgactcgag ctcgtcctca tcgacgcccg ccacccccgc
      121 gtcgacctcg ccggcgtcct cgacgtagcc tggcctccac atggcacgac gcaagcaccg
      181 caccgcactt ctcgatcctc ccccatcggc gcccgtcaag ccgggcatgg tgagccctcg
      241 gcgcaccgtc cccgccggga tcgcccgccc cgagtacgcg ctcaccggcg agccgtcatc
      301 gcgcgccagc cgcgccgtgc gcacgcccga ggagatcgag cacatgcgga tcgccggtcg
      361 cgtcgccgcc gaggtgctca tcgaggtcgg cacggcggtc gctgccggcg tcacgaccga
      421 cgaactcgac cggatcggcc acgacgcctg cattgcccgg ggcgcctacc cgagtccgct
      481 caactaccgg gggttcccca agtcgctctg cacctcggtc aacgaggtca tctgccacgg
      541 catccccgac agccgcaagc tcctcgaggg cgacgtcgtg aacgtcgacg tcacggcgtt
      601 catcgaaggg gtccacggcg acaccaacgc caccttcgtg gtcggcgagg tcgaccacgc
      661 gtcgagggac ctcatccgca tcacccgcca ggcgatgcac gccggcatcg ccacggttcg
      721 cccgggttcg cgcatcaacg agatcggccg cgccatcgag gcggccgtca agggcggcaa
      781 ctacggcgtc gttcgtgagt tcatcggaca cggcatcggc gatcagttcc acaccagcct
      841 gcagatcccg cactactacc aggcgagtgc ccgtacggtc atcgaggagg gcatgacctt
      901 cacgatcgaa ccgatgatca cgatcggcag tcccgccctc tacgtgtggg acgacgactg
      961 gaccgcggtc accttgtcgg gccagcgatg cgcccagttc gagcacacgc tcctcgtcac
     1021 ctccgacggc gtcgagatcc tgacgctgcc cgacggtacg ccgcccgccg aagagctgtt
     1081 cgcaccgtag gtgcgctcgc cgctcctccc ggagcggccc cgaccttccc cttcacgacg
     1141 ccgtgaggaa ggcccgcgcc cgtgcgcacc cgagcgatca ccacccgacc gacccctgca
     1201 cctggccctg cgtcgcatcg tcgtcgacgc catcgccgtc gccggatgcg caccccgctc
     1261 cgcgtccggc tgcggtcacc agcggcctac tggtcgctcg tcgccgtgct cgccgtcggc
     1321 acgatcgtgt ggatcgaacg ggccgagcgc gacctcgcac ggcgagcgac cgcgctcggg
     1381 ccgaccactc aggttgccgt cgtggtgggc gaccacgccg gcggggagca gctcgatgac
     1441 gccgacgtcg aactgcgcga tgtgccccgc tccgcagtcc ccgccgacgc gctcaccgct
     1501 gcgccccacg accggcggct ccgaaccgac gttcgcgccg gtgaggtgct gacctccggc
     1561 cggttcgccg ccggtgggcg atcggagatc gcagcgcaga tcccgccggg acggcaggcc
     1621 atcgccgtgc cccggacctc cgcagcactc gacctccggc ccggcgatca ggtcgccctg
     1681 ctcggactgg ggtcgtcggg agcgtccgag gtgatcgtcg acgacggcct cgtcgtcgcg
     1741 gcgacggaag gtgccaccac gatcgcggtc ccgctcggcg acgtcggcga cgtggccgat
     1801 gccgtgctcg cggggacgat gacggtcgtg ttgtccgggt ccgggtgacg ggtcggggtg
     1861 acggtgacgc cgatgggtca ggcgccgctc atcgacacgt cggcgatgat gaggctggcc
     1921 gcagcatctc ccgacgacag ccattcgagg tcgccaccca cctcgacgat gtcgagcagc
     1981 agacgctgga tcgtcgaggc caacgtcatc tcccgcaccg gttccgcgat cgcaccgttg
     2041 cggatcatca gaccgtcggc gccgacggag aagtcgcccg acacggggtt cacacccgag
     2101 tggagcccgc tgaacccgct gacgtacaac ccgtggtcga ccgatgcgac cagttcatcg
     2161 aagcttcggg caccgggctc catcacgagc acctgcgccc cgacgcccgg cagcgaacgt
     2221 gagccccgca gggccgagcc cgtcgacgcc gtaccggcgc ggcgcccggt gtaggagttg
     2281 tgcaggaacg ctccgaggtt gccggcgtcg atgagcacgt tgcgccggca cgccagaccc
     2341 tcgccgtcgt aggcggtggc ggcgatcgac cgggagtccg tcggatcgtc gacgaaggtc
     2401 agcaacggcg aggcgatctg ctcgcccacc cggtcggcaa agggtgatcg gcccttgaag
     2461 accgactcgc cgtcgaccat gtcggccacg atcccgagca gggtcatcgc cagtcggggc
     2521 tcgaggagga tcgagatctt gccgctcgga ggcttcgtcg cgccgagcag ccgggtcgcc
     2581 cgatcggccg cgtcggcgat cacgcgctcg acgtcgagcg cctcgagcga gcgggcggcg
     2641 tcaccggccg cccccgtctg ggtctcaccg ttgtcgttgg ccaacgcctg gatgccgatg
     2701 ctgcacgatg tgccgcggtc gtagacggag atgccgttgg aggcggcgta ggcaacctcg
     2761 ccggcggaat cgccccaagc ggccacccgc acgccggaga tacgggggtc ccgcgcccgc
     2821 acgtccgcct cgaggcggag cgccagatcg atcttggtct gggcgtcgat gccgaggatg
     2881 gcctcgttcc agtggtcgtg ttcggtcgca gcgatgccat cgggctcggc gagaccgtag
     2941 aactcgtcga cctcaccgaa ggtcacgttg tcgcgggcct cggccagcgt ctccgcgacc
     3001 acgtcgggat cgatcgaccc cgccgacgcg aagccctgcc gaccgccggc gatcacgcgg
     3061 atgcccacgg cataggactc ggcggacgtg aaggactcga cctcaccgcc gaacaccttg
     3121 accgacgtcg accggccgtg tccgaccatc acgtcgatct gttcgccgga agcggcctga
     3181 ccggcgaccc gggacgcgag ctgtcgcagg tcgatgcctg tctcggtcac ggcgtcagct
     3241 ccccgacaac gtgacgtcgg cgatggccag ggtgaccccg gtcgcgtcga gcggcatggc
     3301 cgtgaggtcg gacccgacca tcgagatgtc ggccatcatc cgctggaggg tcgacgcgat
     3361 cgtgacctcc ttgaccgcct cggccaactc gccgccccgg atcaccatgc cctcgatgcc
     3421 ggtggagaag tcgcccgaga cgggattgac gccggagtgg agaccctgga catcggagat
     3481 gtagatgccg tcgccaacgg cggcgaggat cgacgccggg tcgtggtcac ccgcaagcaa
     3541 cgagatcgac tgggtgcctg ctcccggcgt cgacttgaag ccgtgccgaa cggccgaacc
     3601 cgtcgagcgt gcgccgagcg cacgcgcgga gtacccgtcg tgcacgaagc cgctgaccac
     3661 gccggcgtcg atgagcacgt tgcggcgcgt ggcgagacct tctgcgtcgg agggggtggc
     3721 cgagggccag cggccgtcgg tggggtcgtc gacgagcgtc acggtcggcg gggcgatgga
     3781 cgctccgaca cggtcggcga ggaacgaacg ccccttcaac acctcggaac cggagaacga
     3841 ctcaccgacg atcgagagga actgggctgt cacccagggg tcgaacacca cggtgagtcg
     3901 gcggctggga gcccgaccgg cgcccagcat tcgaacggcc cgctcgacgg cctgccgcgc
     3961 ggtgtcctcc agatcgaggt cgtcgaggcc tcgaccgacg ctgaagccga acccggtcgt
     4021 cacgtcgtcg tcgactccgg cgaggctgta ggcagcgagc gagcagccgg tctcgcggct
     4081 gatcgagcgg atgccggtgg tggacgcgat cacggcggtc tcgatggagt ccgcgtactc
     4141 ggccatctcc acgccgagga tgcggggatc gccggcgcag acgagccgtt cgagctccag
     4201 cgcgagctcg atcttccgat ccgggcccag gccggcgagc gcctcgtcgt agaggtgcag
     4261 cgtgacctcg gcgaccccgt cgggctcgac gagaccggtg aactcgtcca ccgtcgagaa
     4321 cgccgcattg tcacgggcct cgccgagggt ttcggccacg acgtccgcgt cgagggtgcc
     4381 ggcgtatgcg aacccctgcc gatgcccacg aatcacccga accccgaatc cagcggactc
     4441 ggcggaggtg aacgcttcga tctcgccgtc gtaggcccgc acttcggtgt cgcgggagtg
     4501 cgccgcgacg acctccacct gttcttccgg cgcggccatc gcgagcactc gctcggcgac
     4561 ctccatcagc tcgtctggac tcatgggtgg cagcctgcca cacccaccgt cggagccgga
     4621 tcacaggacc cggccgggtc ggcgaccggg gcccgtcggg tcatcgtctg gtcatcgtct
     4681 ggtcaccgcc ggccggcagc ggtcccgagc tcggcggcct cggccaacag ctcgaccgat
     4741 cggatccgct cctccaccgt gggcgactga tgggcgacga tcagctcgtc ggcgtcggcg
     4801 tgctcggcga accggtcgag gtactccgtc acctgggtcg gggtgccgac cccggagtac
     4861 tgcgccatct gctggagctg acgaccctgc ggcgagtcga ggatccggtc cgcctcgtcg
     4921 tcgctgtagg accgcccgct gggcaggagc agcgcaacgc gccgccgttt ggtcacctgg
     4981 aactgctgct gtgcgtcggc ctcgtcgtcc gccacgatcg cgttgacagc ggcgatgaca
     5041 tgggggcggt cgagttgctc ggacgggcgg aactccgacc ggtacaacgc aaccgcctgc
     5101 gaaagagcgg tgggcgcgaa gtgcgacgcg aagccgaacg ggaggccgaa cgccgcggcc
     5161 agctgcgcac cgaagagtga cgacccgagg atgtagagcg gcaccttggt gccacggccg
     5221 ggcgtcgcgg tcacaccgtc gacctgccgt tcatcggaca ggtaggcctg cagctcacgg
     5281 acatcctggg gaaagcggtc ggacgattcg ccgttgcgtc gcatggcgcg catggcgttc
     5341 tgatcggttc cgggcgcgcg gcccaggccg agatcgatgc gaccggggtg cagcgactcg
     5401 agggtgccga actgctcggc gatcaccagc ggtgagtggt tcggcagcat gaccccaccc
     5461 gacccgagcc gaatcgactc ggtgtgggcc gcgacatggg cgatcagcac gctcgtcgcg
     5521 gacgacgcga tcgacgccat gttgtggtgc tcggcgtacc acacccggcg gtacccggcc
     5581 cgctccgccg cccgggcgag cgcgacgctc ccctcgaggc tgtcgcgcac ggtctcaccc
     5641 gcgccgacga ttgcgaggtc gaggatggag agggggacgg acatggggac acgctttcgg
     5701 gaggcaggac gacgaacgtg gtcaacgtct ccgtcgggcc cgatatttca ggacccgacc
     5761 gactcgcgac gcgcccacgt ccgggcacgc tgcaactcgg cgatgcgggc gtcgatctcg
     5821 tcgagccccg acgagatcgg ccaaggcccg tcgactccgc gccgacgcgc tgaccaagct
     5881 ctgggaagaa agcgtcatcg tgcggctcac cccgcgtccg tcgacgccgt tgaccatcga
     5941 gcgagcagcg gacggatccc gccggtagaa cgggcgcatg cccgccgcat ccatctccac
     6001 cgtcctcagc cccctccagg gcaccgttgt cgaggttcgc gtcgccgccg gagagcgagt
     6061 tgcccccggc gccacgctgg tgatcatcga gtcgatgaag atggagcacc tggtcgagac
     6121 gcccggcgcc ggcgtcgtcg aacgcatcct cgtctccgcc ggagacatgg tcactgcaga
     6181 ccaggcactc gtcgacctcg tccttacgcc cgacgacgag ccggcaccca gcgccgccgc
     6241 gaccacagcc gacgagcact cgatcgagcg ggccgacctg gcggcggtca ccgcccgaca
     6301 cgcgacgggc ctcgacgccc accggcccga gagtgtgcag cggcggcgac agcgcaacca
     6361 gcggaccgcc cgcgagaacg tcgacgatct cgtcgacgag ggcaccttcg tcgagtacgg
     6421 cccactggcc atcgcgtccc aacggggtcg gcgaccgctg gaggagctga tcgagcgcac
     6481 gccggccgac ggcctcgtgg gcggcgtggg cgagatcgag ggtcgaccgg cggtggtcct
     6541 gtcctacgac aacacggtgc tcgccggcac ccagggccat cagaaccacc gcaagaagga
     6601 tcgactgttc gaggtcgccg agcggcagcg gttgcccgtg gtgctgttcg ccgagggcgg
     6661 tggaggacgt cccggcgaca ccgacgtacc cggcgtgtca ggcctggacg tcatggcatt
     6721 cgacctgttc gcccggttga gcggatgggt tcccctcatc gggatcgctg cgggcaggtg
     6781 cttcgccggc aacgccgccc tcctcggctg ctgcgacgtg atcatcgcca cgctcgactc
     6841 caacatcggt atgggcgggc cggcgatgat cgagggtggt ggtctcggtg tcgtcgcggc
     6901 cgacgacatc ggtccggtcg gagtgcaggc gcccaacggg gtgatcgaca tcgtcgttgc
     6961 cgacgaggcg gccgcggtcg ccgcggcgaa gcagtacctc tcctatttca cgacgtcggt
     7021 gacgcccgac cactccgccg tcgaccaatc ggtgctccgc gaccgcgtgc cggagaatcg
     7081 gctccgcgtg tacgacgtcc ggcaggtgat cgaggcgatc gccgatgccg actcggtcct
     7141 cgagctgcgg accgggttcg gcaccgggat cgtcacgacg ctcgcccgcg tggaaggacg
     7201 ctccctcggc atcgtcgcga acaacccgtc gcacctcgga ggtgcgatcg acgccgacgg
     7261 ggccgacaag gcatcgcggt tcctccagct gtgcgacgcc ttcgacctcc ccgtcgtgtt
     7321 cctgtgtgac acccccggca tcatggtcgg acctgcggcg gaggagacgg ccctggtccg
     7381 ccacgtcaac cggttgttcg tgaccggcgc gaacctgagc gtgccgaccg gcacgatcat
     7441 cctccgcaag ggctacggac tcggtgcgat ggcgatgtgc ggcggcagtt tcaagtcgcc
     7501 gctgttctgt gtggcctggc cgaccggcga ggtcggcggc atgggcctcg aaggcgccat
     7561 caagctcggg ttccgacggg agttggacgc cgtcgccgac ccgacggaac gcgctgcgat
     7621 gtacgactcg ctcgtcgccg ccgcgtacga acgcggccgc gccctgaacg tagcgagcca
     7681 cttcgagatc gacgacgtca tcgaccccgc cgattcgcgg cgttggatca ccaccctgtt
     7741 cacctcggcg gcctccgacc tgaacacgcg ccaccgcctt ccgaagaagc gtccctacat
     7801 cgacacatgg tgaggtcgcc cgaggccgcc cccgaccccc gacctgacgt tgagtggtta
     7861 tagggctact ctccgtggcc atgaccagac aacgactcct cgcagccatc gttccactcg
     7921 tgttcctcgc cagcgcgtgt cagaccgagc aggtgatgtg gggggcgtgt accgccggca
     7981 acgacccgtg gggaaccgac ggccggtacg tgctggcctg ccagaacggc gagtggatcc
     8041 ccgtcatgac ggtcgccgaa ttcgtggcca tcaagcaggg caagcaggtc aagatcgctc
     8101 cgctgcccac caggccgacg accacgacca ccacaacgac caccaccacg gccgcgccga
     8161 ccacgtcgac cacctcctcg acaacgagct cgaccacgtc gacgacctcc acgacctcga
     8221 ccacgtcgac gaccgtcccg atggcctcgc cgtcgatctc gttcgtcaac cctgacgagg
     8281 gcccgaccgc tggcgggcag gccaccgtca tcaccggcac gggcttcacg ggcacgaccg
     8341 cggtgacgat cgccggcacg agcgcgtcct tcaccatcga ctcggacacc cagatcaccg
     8401 ctacgacccc ggcccacgcc gccggcgccg ttgcggtcgc cgtcgccaac ccgtcgggca
     8461 ccgacacctt caacggcggg tacacctacg tgtcgatgcc gacgatctcg ggcctcagcg
     8521 tgtcgaccgg cccggtctct ggtggcacca cggtgatcat caccggcacc aacctgagcg
     8581 gcgtcggcag tgtggacttc ggccccaccg ccgccgcgtc gttcaccgtc acctcggcca
     8641 cgcagatcac ggcggtctcg ccggccggtg cggcaggcgc cgtcaacgtg cgggtcacgt
     8701 cctggggagg cacgagcgac gtgtcactcg cggacgaatt cacctacgtc tgaccgggag
     8761 tccggccgac cggccggccg gccggtgcag ggttcagccg gcgagcgccg tgcggccaag
     8821 ctcgctggcg acgacatcgg catggtcgac gagcactccg gcggcagctg cgtgttcgcg
     8881 ccagcgtccc acggctgcga tgacgtcgtc gatgacatcg tcaggctccg gcaccttggc
     8941 acgttcggcc aggtcagcga gcgtgggacg gtcggcgccg tcaacggacc cggagatcct
     9001 caactggtgg tgcgcgagcc aaccgccgcc gtcgaagaac gcgaaggtca ggtcgtacgc
     9061 cggtgcgagg cgccattgct ggttctcgta gatgaacgcg aagttcttcg tgtggtcgtc
     9121 gcgcacggcc gcggcgatgt tgaagacgac gtgtcggaag gcgcggcgcc ggtcgtcaac
     9181 gccgagttcg agccgaccgc aggtttgcat gaactgctcg tagctgtgag cgccggggtg
     9241 ttcgggtgga aggtgcgcga gcgcagcgag cgactggacg tgcaacttcg cactcggggt
     9301 cggccggtcg aatcggcggg tgaggaagtg gacgagtcca tcaacctcga ggagtcggca
     9361 ttcggccatc tccaacccgg cgtcgacggc catcagatgg taggcgtact cgagctggcc
     9421 atacccggtc gtctgcccga ttcgctggcc tggggacacg tcgaacttca acagccagtg
     9481 agtgaagccg gccggcgcgt cgtgctgtcc ggagcggacc tcgccgtccg gcccgattgc
     9541 cactgcagcc ttggcccgcg cgccgccggc ggaaccggac gtgtccagta cctgctcgag
     9601 ggtcgagccg gcgtcgtcgg cctgcaacgt cccacggaga gcgctgcggg cggtgtcgac
     9661 cgcttcggcc aggtcgacga ccgacgcggt cgaagcaagg tcgtcgctga ccgacgggtg
     9721 gaacgtcacg gcaccgacgc ctcgcgctcc gatgtagccg agtcggtcga cgacggacac
     9781 ctggccgggt tggatgccgt ggcgggcgag ccacgcagtg gtcaacagat cagcgaacgt
     9841 gccgggaagg ctgtctgcga cgagaccagg aactccgcgg aacgacgtgt tcgcgagttc
     9901 ggggaacagc cggggccccc gcagtcgggg cagagcgatc ggggagaggt tctcgccgga
     9961 gtcgacccag tccgggaagt actcgaacac cggcacgcct cgggaggggt cgacggccac
    10021 cgcaccgact gaccggtcga ggtagcggac ctcgacggct gggagctgca cgaacgggtc
    10081 agccatcggc gcgccgtgcc cgttggcgtc ccgtacgtcg tcgagatgcc tcgagcacgg
    10141 cgatcgggga cacgggctcc acgggggaaa gcgtgtccag ccaggattcc gagtccaggg
    10201 cacgcaggac cttgatgagc gtcgagaccg atgagccgcg tccgtgttcg aggttctgca
    10261 ccgtcttcgc tgcgatccca gcctcagcgg cgacgtcctg gaccgtccgg ttggaccgca
    10321 gacgtcgagc gcgcacttgt gcgccaacgc gtccttcgag ttcctcgacc gtctcccaca
    10381 tcacgaccac ctccggtaat atcttaccgg ttatggtcct tcttgcagga tattcggcag
    10441 tattctaccg acgaagacgc tgacggagtt ccacatgctg acgatcttcg tcgctcagga
    10501 cgaagatcgg gctcaggtgg actcctacgc cgcggtgccg ccgatggtga ggccgtgcac
    10561 ccggagcgtc ggaacaccgt cgccgaccgg gacgccctgg ccgtccttgc cacaggtccc
    10621 gggcgggccc atcgcgaagt cgtcggcgac cgcgtcgatg ttcaggagca cctcgggacc
    10681 gttgccgatc aggttgccct cccggagcgg ctcggtgatc tcaccgttct cgatgaggta
    10741 cgcctcggtc atgccgaaca cgaagtcgcc ggtggcggtg ttgacctgcc cgccacccag
    10801 gtgcgcgatg tacaccccac tctcggtgtc ggccacgatc gagtccttgt caccctcgcc
    10861 gcccaacaga taggtgttgg tcatccgaac catcgggagg tgctggtagc tctgacgccg
    10921 cccgttcccc gaactggcgc gaccctcctt acgggcccgg aggccgtccc acatgtagtc
    10981 gacgaggatg ccgtcctgga tgagcacgtt ccgctgcgcc gcccgaccct cgtcgtcgat
    11041 cgcgaaacag ccccactcgc cgcccatcgt gccgtcgtcg acgagggtca cgagcggcga
    11101 cgcgacctgt tcgccgatgc ggttggcgaa caccgacgcc cctttggcca cgaggtcggc
    11161 ctcgaggccg tggccgcagg cctcgtggaa catgacacca ccgccgccgg aaccgatgac
    11221 gaccgtcatc tgtccgctcg gcgcgggacg ggctgcgagc ttggtgatcg cgcggttggc
    11281 ggcgttgcgg gccagctcct ccacgtcgac ctggtcgaac aactcgaacc cgacggtgtg
    11341 accgacggac tcacggccgg tctgcatccc cgtgtcgccg gtcgcgacgc acgagatgga
    11401 gaagacggtc ttgacctggt cgtcgtcggc gaacgtgccg tcggtgttgg cgacgaggat
    11461 gcggcgccgg gagtcggaat atcggaccat cacctgagtg atggccgaac cctgcgagcg
    11521 ggcaacctcg ttggcgcggg tgagcagctc gaccttgcgg gccttgggga cgtcgccggg
    11581 aagcagctgc acgacgttcg gacggggcgc ggacgtcttc gacaggtcga cgaccgtcgt
    11641 gccggagccg ctgccacggg cggcggccgc ggcgatctcc gccgtggcga tgaggctggc
    11701 ctcggacagg tcggcggtgt gcgcgaagcc cgtggactcc ccgaccacca cgcgaatgcc
    11761 ggcaccacga tcgcggcccg acgtcagttc ctcgaccttg ccgtcgtcga gtactgcgct
    11821 cgacgagcgc ttgtcctcgg cgaagacctc ggcgaacgaa gcgccaccct tggtggcagc
    11881 gccgagcacc cgctccagga ctgattcgtc gatcaactga actccagtgg ttgagtgcgg
    11941 tactgcgtgg gaagcggatt gcgatctggc ccggtgccgc aagagtcctt atggtatccc
    12001 cagtgatgtt ggacaccggc ctcgaagcaa ccgttgaact cgtcgtcgcc gacagcgaca
    12061 cggcgcaggc catgggctcg ggcgatgtcg ccaccttggc aacgcctcgc atgatcgcac
    12121 tctgcgagca ggccaccatg tcagccctcg acggccacgt cgacgccggc ttcaccaccg
    12181 tcggactgtc cgttcaggtc gaccacctcc gccccaccgc cgtcggcgga aagatctggg
    12241 cagaagcgca cctcgacaag gtcgagggcc gccgtctgtg cttcagcgtc gcggccaagg
    12301 acgagcgcgg tctcatcggc gccggccggg tcactcgcgt cgtcgtcgaa gtcgagcact
    12361 tcatggaaaa aacgcgctga cgctgcggta cctcggcgat ttctcaagct gccgttcgaa
    12421 catgtcgatc actgacgtgt gggatcccag aagcgcgcca cgtcgatcac cggcctcgga
    12481 ctgatcagcg tcggcgcggc attcaccctg gtcggcgggc tcggcgtcgg attccgagcc
    12541 gccggcctcg gctacctcgg gcagagcctc gtcgccctga ccctcgggct cgggatgctg
    12601 tgggtcggcc tccgcgtcgc cgcggccgct cggcgggcga tcttcgaatc ggtccgcacc
    12661 acaccgatgc gagcccacac cgtctacacg actgcggcgc ctcagcccgc ccgggtgcgg
    12721 gtgtcggcgt ggacgtcgcc ccagctcgcg ctgccacccg cctcttcgag gtgacctctg
    12781 gccctactcc ggggccgatg atccgcgaga ctgggtgagt gcccatcctg agtgcgcgag
    12841 gcgttcgcaa gatctaccgc accggcgctg tcgagttcga ggccctccgt gggatcgaca
    12901 tcgatctcgg ggccggcgag atggtcaccg tgatgggccc gtcgggcaac ggcaagacca
    12961 ccttgatgaa ctgcctgtcc ggcctcgacg acatcgacgc cgggaccgtc gtcatcgacg
    13021 gtgaggacat ctcggcgatg agcgatgcgc gccgcaccga ccaccgagcc ggccgcatgg
    13081 gtttcatctt ccagagcttc aacctcatcg gggtcctgtc ggccgtcgag aacgtcgagc
    13141 tcccgctcct gctgggcggt tccaaaccga aggcagcccg ctcccgggcg cacgacatgc
    13201 tgacccgggt cggactcgac ggacgcgaca agcaccgccc cgccgaactc tccggcggcg
    13261 agcagcaacg tgtcgcgatc gcgcgtgccc tcgtcagcga accggcgatc atctgggccg
    13321 acgaacccac cggcaacctc gacagcacga cggccggcgc ggtgctcgac ctgctgctcg
    13381 aagtgcacag ctccgggcag acactcgtga tcatcaccca cgacgccgac atcggagcgt
    13441 cgggcgaacg gctcatcgag gtccgcaacg gccagatcga gtacgacggc tcgccggacc
    13501 acgcgcacca cgtgcacgca cccaggtgag caggtctcga tgaacccggt gtggatcgtg
    13561 gtcggcatcg ccgtgctcgg cctgctgatc accgccgtgg atctcgccgc ctcccccacg
    13621 taccgccggc tcgcggtccg gagcatctcc cgccgtcgcg gcgaagcgat gctcatcgtg
    13681 gtcggggcga tgttcggcac cgcgatcatc ggagcggcgc tgatcgtggg cgactccttc
    13741 gacgggtcga tccgcgacat cgcccggacc gagctcggtc cgatcgacgt ccgtgcccag
    13801 atccatcccg acgacgacgt ccgccgcgag gtgctccagc tcgaggaccg agtcgcccac
    13861 tcgggcatcg accacgtcga cgggttcctc cccgtcgtcg acgccgcagc ggtcctcgac
    13921 aacggccggc ggggggccgg gcagcgcatc gatccgacga actgcctgct cgagatggac
    13981 ttcacgcagg cccgccggtt cggtcccgat ccgatctcgg gcatcgaggc ggccggtgac
    14041 acccccgccg agggcgaggc ggtgatgaac cgggacgtcg ccgacgttct cggcatcacg
    14101 gccggcgacg acctcaccgt ccacctctac ggccgctccg agacgttggt cgttcggacc
    14161 atcgtgccga ccatcgggct cgcggggtac tgcggagcga tcgtcgcacc cggcaccatc
    14221 gagtcgatgt ggacggcggg cggaggtgcc accaccgccg gcactgcgcg aggccccacg
    14281 ggcgagctgt tcgtgtccct cgagggtggc gtcttcgact cgaccaggta ctccgaacgc
    14341 gccgagcgac agatccgggc ggcagtgaag gccggcgggt tcaccgatgc caacgtcgac
    14401 ccggtgaagg ccaaccggct cgagagtgcc gaacgcaacg gcagccagct ccgcaccatc
    14461 ttcagcggca tcggcgggtt cagcgtgatc tccgggatcc tgttgctggt caacctcgtg
    14521 atcatgctgg ccgaggaacg aaaggtggag ttcggcctcc tcagggccgt cgggttcaag
    14581 cgtgctgcgc tcctgcgcag cttcagcctc gagggctcgc tctacgcgct cagcgcagcg
    14641 gtgctcggcg cgatcgtcgg cgtcggcgtg ggggcgctgg tcatcgaagg cacccagaag
    14701 atcttcgccg agcccaattc gacgttccgg atcgacctgt tcgtccacgc tcgcacgctg
    14761 ctcctggcgg ccggcctcgg gtacgccatc tcgatcgtga cgatctggtt ggcgagcgcc
    14821 cgggtgtcgc gactcaacat catctcgtcg atccgcgacc ttcccgcccc tcgccgctcc
    14881 ggacgccacc tcggccgcat ctcgctcgcc ggactcggtg tcgtcgccgg gagcatcgcg
    14941 acggtggtcg gggtccgtgc cgggtcgcag atcccgctca tggcgggtat cccggtcgcg
    15001 tcgttctccg ccatcggggt ggtcacgcag atgaacttcc actcgcaacg gccggtgcgg
    15061 gtggcgtcgg tggtcggccc catcgcagcg atcgcctgga cgctcggcgt gttcaccctc
    15121 ttctccaccc agatggagca cgccggtatc gccgtgttcg tcgtgatggg cctgctgctc
    15181 gtcgccgccg cggtgatcat cgccgtcgcg ttcgcgccgt tgtggggcca cgctgccgcc
    15241 gcacgcggcg ggtcgttcgc gctgtcctgc cgtctcggct tcgcctaccc cctcgcccgg
    15301 agcgtccgca ccggcctcat cctggccatg ttctcgctcg tgatcttcac gatggtgttc
    15361 atggccagcc tcgcggcgac catcgacggt caggcgtcga gcgctgcggg cgatctcgat
    15421 gccgggtacg acatcgtcgt cgacacgaac ccctccaacc cggtgtcgga gaccgatctc
    15481 gggggacggc ccggcgtcgc cgccgtgacg acgttcacgc agtccgggcc cctgttcacg
    15541 aaccgctacc agcgcgagcc cacccgctgg ctcgtgtcgg ggttcgacga ggggatgctg
    15601 cggttccgtc cgccggcgtt gagcaaacgg ctggatcggt ttgccgacga ccgctcggtg
    15661 ttcgacgccg tcctcgacga cccgacgttg atcgtggtgg acgaccagtt cctgctgcgc
    15721 ggcggcgggc ccaaggacga tggtcccgag cccggcgacc gggtcgagat gaccaaccct
    15781 gccggtgatc ggcgaaccgt cacggtggcg ggtgtgctga agaccgacgc gatcttccag
    15841 ggctcgctgt ggcccaagac cgcagtggcg gagttcatgg caccggcaac gagcgagacg
    15901 cgggcgttcc tggccgcagc ccccggttcg acgccgagcc gcctcgccgc gatcgcccgg
    15961 gaccttcagg cagcgaccgt cacccagggc accgacgtct cgacgttcac ccagatcgtg
    16021 gccgacgagt tgggacgcac cacgtcgttc atgaggctcc tcgaggtgta cctcgggttc
    16081 ggcctcctga tcggcatcgc cggcctgggc gtcgtcatgg tgcgggccgc gcgggagcgg
    16141 cgccacgaga tcggcacgct ccgggcgatg ggcctccagg gccgagtggt atcccgggcg
    16201 ttcctgatcg aggcgttctt catcgccggc ggcggcgtga tcatcggcac gagcctcgcc
    16261 ctcgtcaccg cctaccaggt cgtggtgaac tcctcggcct tctcgatcgc cgccggatcg
    16321 ttcgtggtgc cgtgggtgcc actcgtgctg atcgccatcg tgcccaccgg gttcgcgctg
    16381 ctcgccgccg gccagccggc ccgggccgtg ggccgtatca ggccggcggt ggcgctgcgc
    16441 atcgccgact gacgaacgcc gcctcaggca accgcggtgg cgcgtgacga cgctgcgccg
    16501 aggaactcgt gcaacgagga acgccacgac tcggtggact cgaactgcgg gcagtgcgcc
    16561 ccgtcgggat gcacgaccag ttgcgccccc ggaatcgtcg ccgccatccg ctccgatgcc
    16621 ccgacgaacg cagcgtcgag ctcgcccacc tgcaccagcg tgggcaccct caccgagccg
    16681 agggcgtcga ggcgatcgtc gatcgtcgac atctcgtgca gcatcgccgc gtacatcgca
    16741 gcggagcact tgagcatctt gttctcggac cactcccggt aaccgggacg ggcggcacac
    16801 gctcgctcgt aggccgggtt ctccacgtcg ctcatctgca ggatctgctg gatcacctcc
    16861 aggccttgtg tgcgggccaa ctcgatcccg atcgccacga ggtcgaggtc gaggtcaccg
    16921 accacaccgt ggtgggtgtc catcagcacg agccgctcga cccggtcggg ctgctcgagc
    16981 accatcacct gggcgatcat gccgcccatc gagtggccga gcaggtcgaa cgacgcccag
    17041 ccgagccgtt cggtcagggc gaacagatcg tcggcgaagg aacggagacc gtaggaggcc
    17101 tcgtcgtcgg gctgatcgct ctcgccgtgc ccgcgcagat cgggggcgac gacgtggtac
    17161 cccagctcgg caaggccgtc gacctcctcg gcgaagtcct ccttgcaccc ggtgaagccg
    17221 tggaccagga ggaggggggc gccaccgact ccggcttcga gggcgtggag ccgcacggca
    17281 ccgaggtcat gggaggtcgt gtccataccg agaacgtacc cggtgcccgt cggttccgct
    17341 ccgacggcgg ccgcctggaa tactggcgcc atcgagccgg accctccact cctcggcgtc
    17401 tccaccgtcg acctcgccgc agagctcgcc ggggaggacg acgggcaccc accacgccga
    17461 cgatcgccgg tcctgaccct ggtggtccgc gtcctgctga gtgctgtgat gctggccgtg
    17521 ctcgtgtggc gcctgccgga agtgcggtgg agcgacctcg gcccacgatg gcgctccacc
    17581 tcggcggtct ggctcgtcgc cgccgggctc acgatgagcg cggcgttcgc cctcagcacg
    17641 ctccgttggc ggcgggtcct ggtcgccatg cgtcccgctc cccgctaccg gcgcatgttg
    17701 tcgcacttcc tcgccggcca gttcgtctcg aacgtgctac ccaccgcgtt cggcggagac
    17761 gtcatccgcg tgagccgcct cggccgcgac atcggcgatc atccgacggc gttcgcgtcc
    17821 gtcacgatcg agcgcatgac cgggtggctg gtgctcccgc tcatcagcgg cgtggccctg
    17881 ctcgcgcagc ccgacctgtt gtcgctcggc tcggcgacgg ccctcgccgt cgcgatcgac
    17941 ggcggcacga tcgtcgccct cgtcgtcgtg ctcctcgtcg cgtccaaccg acgctttgga
    18001 gcctccgtcg agacggtgac gggatggcgg cgattcttcg tcgcgatcca cctgggcgtc
    18061 gacgccctgc gccgagaccg gcgcggcagc ctcgaggtga ttgcggccgg tgccgcgttc
    18121 caaacgctgc agtgcctggc ggtctggatg gccgccgaag cgatcggcct gcagggccag
    18181 gtgacggtcg gcgtggcgct ggcgttcttc ccggcggcgg ccatcgcaca gaacctgccc
    18241 gtcggtctcg gcggcctcgg ggtgcgcgaa ggcatcttcg tggtgttctt cggcgccgtc
    18301 gaggcgccgc gggccctgtc gatcaccctc ggactcgtcg tctacgggtt gacgatcgtg
    18361 acgagctcgc tgggagctcc ctcattcgtc atcggaagcc ggacaggttc gcagaatcct
    18421 cacaccgggg atgtcacacc gcccctcggc gggtcataga ttgggctcct gacccaaagg
    18481 cggaggtgcc tctccctcat gctcctggac aacatcgacg gccccgcgga cctccgccgg
    18541 ctcgactatc cagcgctcga ccaacttgcc gacgagatcc gcaccgtcgt cgtcgacgcc
    18601 gccacgcgcg ccaagggcca cctcgggtcc aatctcgggg cggtcgaact cacgctcgcc
    18661 ctccaccgcg tgttcgactc ccccaacgac atcatcttgt gggacaccgg ccaccaggcc
    18721 tacgtgcaca agctcgtcac cggccgccgc gacgcgttcg attcgctccg ccaacccggc
    18781 gggctctcgg ggtaccccag ccgtaccgag tcgtcacacg actggatcga gaactcccac
    18841 gcatcgaccg tgctgtccta cgcccacgga ctcgccacgg cacagcacct cgccggtgat
    18901 cccaagcgcc agatcgtcgc catcatcggt gacggctcca tgacgggcgg catggcctac
    18961 gagggcctca acaacctcgg ccactccggt cgcaacgtga tcatcgtcct caacgacaac
    19021 ggtcgcagct acgcacccac cgtctcgcgg ctcggcgaga gcctgtcgcg gctccgttcc
    19081 aacccgatgt accggcgcca gcaggcgcgc ctcgagaagg tcctcggcga cctgccgatg
    19141 gtcggcggat acctcgaacg aggggtcgac ggagcgaagg ccgcggtgcg cgagatcctc
    19201 gaaccgcccg cgttcttcga gaacctcggg gtgaagtacg tcggcccgtt cgacggtcac
    19261 gacatcgccg ggctcgagcg ggcgctccgc aacgcccagg cgatcgacgg cccggtggtc
    19321 gtgcacgtcc tcacccagaa gggtcggggc tacgcccccg ccgagaacga ccaggtcaag
    19381 gccatgcacg acatgggtga ggtcaaggac ggctcgtaca ccgccgcgtt cagcgaggcg
    19441 atgctcaagg agggcgagcg ccgtccggag gtcgtcgcga tcacggcggc catgcccgac
    19501 tccaccggcc tcctcccctt cggcgagcgg ttccccgggc ggctcatcga cgtcggcatc
    19561 gccgagcagc acgccgtcac gtcagccgcg gggatggcca tggggggcct ccgccccgtg
    19621 ttcgccgtct actccacctt cctgacgcga gcgatcgacc aggtcaacct cgacgtcggc
    19681 ctccaccacc agcccgtgat cttcgccctg gaccgtgccg gcatcaccgg cgacgacggg
    19741 gcgtcgcacc acggtgtgct ggacatggtg ctgctcacga aggtgcccga catgacggtc
    19801 ttcgcaccgt cgtcgtacgg cgaggtcggt cagatgttgt ccgacgccct cgacctgtgc
    19861 acgggccccg cagcgatccg gttcccgaag acgatgccgc cgacgaccga gccgggcgac
    19921 accgggtcgg ggctcacggg ccgcaaggtc cgcgacggcg acgagatctg catcatcggc
    19981 gtgggcaaga tgctcgaggt cgcccgcgag gcggccgacc ggatggctga gtcaggaacg
    20041 tcggtgaccg tctgggatcc ccgcgtggtc aagcccctcg acgcggacat gctggccgac
    20101 gcggcccgtc accgcctggt cgtcacgatc gaggacgggc tacgcgatgg cggcgtcggg
    20161 tcggcgatcg ccgacgcact gcgcgacctc gctccggtcc agggcccgtc ggtccgcgtg
    20221 ctcggtgtgc cgtcggcgta cctgcagcac gacaagcccg atcagctcct ggcccgcctc
    20281 ggcctcaacg ccgatggtgt cgtcgccgag atctccgcct ggcagcgagc ctcccaggct
    20341 cactgagcat caggtcgccg aggtaccgga gcgatcgtag ggagcgcagc gaccgtgccg
    20401 agcgaaggtg gccgaggccg ttacttatag aacgggttct cgccggcttg gtggtcggtg
    20461 gcgtcgatga cgtccaacac gtcgggaacc aggtccttga tctgacgctt gatgccctcg
    20521 gcgagcgtga gcgcgctggc cgaacagccc tggcagccgc cgcccatctt cacgtagacg
    20581 ttgttcgact cgtcgacgcc gaccaggctg gcgaacccgc cgtgggcagc gaggctgggg
    20641 ttgatggcct gctcgaggag ctgctcgacc tgctcggcga tcgtgccctc gaggtcgagg
    20701 tcgatgccgg cgagcggatc gggacggttg gggttgcgga tgacgaggcc gccctgaccg
    20761 gccgtgcggg gcaggtcgag cacggagccc cggagcttct cgaccgtctc ggccgggacg
    20821 atcacggtga cgccgctggc ctcgatgagt tggtcgtcgg cgacggcgtt gctgatctcg
    20881 tcgaagctca ggtcgtacgt gtactccgag ccgcgtgacc cggtgatctc gatccggaga
    20941 cccagttcgt cggggttgtc ctcgccggcg cggatgctca gcaccgtctc gagggccgcc
    21001 tcggtgatct cgatcaccgg cagatcgttg gcgaagggac tgacgacgga gccggcagcg
    21061 aacggactga cgacggagcc ggcagcgccg gaactggcga cgggctcggc gggggcagaa
    21121 ccggatccaa tggtttcggt gtcagcagac acaggtactc ctcgactgag tggccacgga
    21181 gaagggccgg gattcagggt agcaagcgcc tcccgacggg aggaaacagg cgcgagctcc
    21241 tcgatgttcc ccgccgaccg atgccggacg agcccgagct aaccccggag gagcgccgcg
    21301 gtgcgcccgt cgagccacgc gtcgagcgcg gacaccgaga tcgggttcat gaactcgagg
    21361 tgcgtgagga agggtttgag gatgttgccg tacagctccc ccgtcggcac ctggccgtac
    21421 cctcgcccgt cggtcggcat cgtcgaagac cgggacgacg cgagccacga cgggatcgga
    21481 cccgcctggc gaccgacgag cggcaggtcc aggagcgaga tcattcgttc ggtgctggtg
    21541 ttgggaacga tgccgtcatc gacggcgtac gacaggaaga tcggggtgcc ccgctgccgg
    21601 atgcgatcga ggtagtaggt gttgtcggcc cggtcgagca ggttctgggc aaagaaggtc
    21661 aacacgtggg tctcgccgta gggcgctccg gtcggcacga ttccccggaa catctcccac
    21721 accagcgagt ggaacagcgt gtcgatgatt cccgatccgg ggacctgcag gaatgctccc
    21781 tcgacttcgg gggcgagcgc gaggaacgtc gcgcccagca cgccgccgag cgaggtgcct
    21841 tcgtagagaa cgtggccgac gtcgagatcg gccacaccgt cgccgccggt gccggcgagc
    21901 cagttgcggg gcaggacgtc gagcgagccc aggtgcacct tgatggcgag gagcagcgac
    21961 agctggtcga gctcgccctg gagcagcagc gactcgaccc gtcccaagag ggacgggtaa
    22021 gcgaggtcga tgatgtgacc cccttcgttg atacgggacc cgtggttggg aacgtcgatc
    22081 ccgatcgtgg cgtagccctt cgccgcgttc tggccggcca cgacgagcat cgactccttc
    22141 atcaccgtga tgccgtgccc gtacatgatc accggagcgc ccccggtggc ggccgagcgt
    22201 tccggaagca ctgccatgaa gtcgacccac aactcgtggc cgacggcggt gccgtcgcgg
    22261 gagatgactc ccgcggcgtc gcggaagtcg gtgatgcgaa cctgccccga gagggcggcg
    22321 gccccaccga tgagcgacgg gcgcacctcg acgtttcgga tcggatgatc gtcggcgcgg
    22381 acgagaccgg cgagacggtc cacatcggcg acgatcgact gctcggtgcg gacgacgaac
    22441 gacgtcgcgg tgaggtagtc gtcccacggg atcgagggct cgacgcgcgc agcgacggca
    22501 cgggtggccg gcgacgcctg ctccagcgac ggcgacgcgt gcgccggagt gccgtcgagg
    22561 gcggtcacgc cgcgtcgcag ggcagcgaag atctggtgcc cgtacgcgaa cttcaccgcc
    22621 ggccacacga gcacgagatt gtgcgacccc cgctcgtcgg cgaggtagct cgacacctcg
    22681 gcgcggaccg ggacccgctc ccccgtggtg gcatcgacga cacggacgag ctcgccgcca
    22741 tccgcaggca cgctgccgac gccgagcgcg tcgggcagtt ggaagacgat cggggtgagc
    22801 ggcgagaacc cgtcggcgcc gatcaatgcc tcctgggtct cgtcacgcgg acccagttgg
    22861 tcgagcacgt ctcgcgagat cgagttgggc ggcacctcga gccggatccc ggtcgctgtc
    22921 gaagcgtcgg ggatctgcca acgatccgac ggaaacggca gggcgcacga gaaggacggg
    22981 gcgccgcacg tcgacggctc cgaacgaggg ggcgctgcga ctcctccgaa cagaggatca
    23041 gggccgacac agccgaccaa cgagacggtg aggagcgcgc cgacggccgc agcgccgagc
    23101 cgcggccgtc gagacgatgt cattcggatg tcccccatag gtcgccg
//
LOCUS       DAOSOA010000015        22263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_2337_length_22263_cov_7.162059, whole genome shotgun sequence.
ACCESSION   DAOSOA010000015 DAOSOA010000000
VERSION     DAOSOA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 22263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22263
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2337_length_22263_cov_7.162059"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..494)
                     /gene="csrA"
                     /locus_tag="PKY13_02260"
     CDS             complement(261..494)
                     /gene="csrA"
                     /locus_tag="PKY13_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOT78776.1"
                     /translation="MLVLSRRSNESIVINGNITVTVLEIRGDHVRIGIDAPREITVHR
                     EEIHAELARANQAASGTATTDLPRLPRPPRPSA"
     gene            complement(495..914)
                     /locus_tag="PKY13_02265"
     CDS             complement(495..914)
                     /locus_tag="PKY13_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOT78777.1"
                     /translation="MLIQSERFGDVDCEESSTLTFPHGLLGFEDQSSFVIVPVGDDGV
                     YSWLQSTSDPGLAFLVTAPHFFFDDYAPEVNDKDLEELDLRDESETLLLCLVTISDDD
                     ITANLLGPLVVNTRTRRARQVVLSEARWSTRVPLSGS"
     gene            complement(925..2304)
                     /locus_tag="PKY13_02270"
     CDS             complement(925..2304)
                     /locus_tag="PKY13_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78778.1"
                     /translation="MLDRRPVAGTGSRELRYEGFPLEAVLARVHAAHGADVTITAADR
                     VRRGGIGGFFAKELFQVTVTVPAPADDGDAELNFVPLGRMSIFGDDAVSDLEVIGDEP
                     DYDEIDPEDVAGALSLEPAPVRDDADAEIWALLAKAAVSTPPAPAAPQVQASVPVPLP
                     VPVAVPAPVPVPVPVPAAGTIAPAVVAPVAQQPEIVAPIHSAGPIASTTLIPASSVVG
                     TPFDPAQVTPPFPGVAPAAVRLPVTTTLPVSAEAAGSTGTIRAGARVDLNGLIARFET
                     VPPAPPLPTAGIVAVIGSRTEALEVAGGLAARLGIPTDDVIVASPPADVVVPTPEDAM
                     RLAATTRYRCAPVAVIVLELVPGRQGHEWVRVVLDGLRAEQVRLAADTTRLIAHQHLS
                     IAAVGGVDVIDLLGAAEHPAPDDALQLPTPVASIDGRPATPELWAATVLAGRATSGAT
                     VDRSGVTGR"
     gene            complement(2297..4345)
                     /gene="flhA"
                     /locus_tag="PKY13_02275"
     CDS             complement(2297..4345)
                     /gene="flhA"
                     /locus_tag="PKY13_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOT78779.1"
                     /translation="MPKKLGASLFPMAVVFVVALMVIPIPTVVLDVLLAFNLGLAILM
                     LLASLNVSKALDLASFPSLLLIATLFRLGLNVSTARLILSEGDAGEVIKAFGSFVIGG
                     SLVVGLVVFFILVVIQFVVITNGATRVAEVAARFTLDAMPGKQMAIDADLNAGVIDDI
                     EARRRRNEVTAEADFYGAMDGASKFVKGDAIAAIVITAVNLIGGLLVGVIQRGQPIGD
                     AVQTYSLLTVGDGLISQIPALLVSISSGLIITRAASADDLGSDVTSQFARQHRAIRTG
                     GLAMMAFAVAPGIPKIPFLMVGGGAYLLGRRLGKAAEALEQQMLELPPVNEEDDDPNS
                     PAELGRALRIEPLSLELAVDLVDLVDPAANGDLLDRVQALRRKLVEELGIVIPPVRTR
                     DNVELELGTYLVRIHGVEVATGHAPNGRFLVIADDLSIYPGPEVFEPVFGLPAKWIGP
                     EHRASAEAMGGTVVDRASVVTTHLAEIAREHAPQLLSRQDVKALIDLVREHDSAVVED
                     LAACGVSLAETQQVLHHLLAEQVPIRDLTRILEVISERARVTRDTELVVEAVRQSLGP
                     TICAARSNNGHLAVITLDPLVEQGLLGTLQRNETGSYLALEPETAARIGRALVTQLRA
                     AEQEGHEPVLVCAPSMRPAMRNFVSRLVPHLPVLSYDELADHLTIDDLGAVTLDQSNA
                     "
     gene            complement(4491..5714)
                     /locus_tag="PKY13_02280"
     CDS             complement(4491..5714)
                     /locus_tag="PKY13_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOT78780.1"
                     /translation="MSGDKHDKTEAPTAKRKKEARRDGQVVRSEDLVTWTSVLIGTIV
                     MPGMVSRTGSAVTAVLRSTVELAHNPDAKMLPTYVGRAMVATLGAILPAMLALLVIAI
                     LGNLAQVGLLLTGKPLKPQMSRLSPFKGIKRIFSVQSLWSTLTATMKLGIILAVSWVM
                     VRGTAADIVRAPFRNASQAVHATAMSALALVRAIAAAALVVGLADYAFQRRKQAKDLK
                     MSKHEIKQEARESEGDPHMKGKRRQMQNQMSRNRMLAAIPSADVIITNPTHFAIAIEY
                     QRALGAPKVIARGADGLAQKIKAEGAAAGVPLVEAKPLARALYAVCRVGEEIPPELYQ
                     GVATVLAFLHQLSATRRTFGGPIGLEVPDTWTPSGSELVRVPPARRRMLEKREAEEAA
                     AAADAGGPRREAAVG"
     gene            complement(5724..6491)
                     /locus_tag="PKY13_02285"
     CDS             complement(5724..6491)
                     /locus_tag="PKY13_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOT78781.1"
                     /translation="MRLSLNPEFLVAFMFATIRCGAWLTIAPPFQGSVPVRIRTGLSM
                     GLGLAMAPRLMQGDTPLSTDTWSLVTGALHNAVIGLVLGFLVYVLFQAIAAGGGMIDA
                     FAAFTSAQLFDPMTKTTTGPVGRLYSTLGTAVLFVTNGHLMLIGGLSRTFELSPVSGF
                     RLDRVGTLITHDVQAFLIAAVQVSAPILGALFVTELLLGLASRAAPKLNIMVLGFGAK
                     SLVLFMLLGAALPLIPYVSAQLLKEAMSVMHALIKGG"
     assembly_gap    6607..6706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6774..7676)
                     /locus_tag="PKY13_02290"
     CDS             complement(6774..7676)
                     /locus_tag="PKY13_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOT78782.1"
                     /translation="MITRIASLSGARTMLQSIQNANSELFRAQEQVTTGRRINRVSDN
                     PADAVSSLNQRATLRRLEQYSRNAEEATSWFDAGDAALADVSDRLAGVRATLVQANSA
                     ANDVTSRAALANQIRAQRESILLASNAERGGRPLFAGNAAGAAAYDANGVYLGDNGAV
                     NLPITGGVTLQVNRTGPEIFGVNNPGDPTQGDLFQLLGALATAVETGDTAAISAGLGQ
                     VDAATKRVATAQVQLGSRASQLEDLTNSLEDSKVSLKSGISNKENIDFAEAVINMKTR
                     EAAYQAAIQATAKVIQPSLMDFLR"
     gene            complement(7723..9135)
                     /gene="flgK"
                     /locus_tag="PKY13_02295"
     CDS             complement(7723..9135)
                     /gene="flgK"
                     /locus_tag="PKY13_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOT78783.1"
                     /translation="MSSFSTLNTALSGLQAHKRVIDVIGHNISNVNTEGYSRRQVLLE
                     PATGMKIASRFDTDFSWNNLGVNIAGVNRIRDQFLDVKARASLASSASAARLDSVLGG
                     VEGTFPEPSDTAIAGQLSAFWNAFSDAANQPGSIPQRAAVLAQASNLAASLNQAAGSL
                     QTQHTDLSSQLTMGVDRVNTLAQQVADLNVQIRAASVAGMDTGDLADERDRLVDQLTS
                     LTGAEVRPIEYDQVNVMLGGSSLVSAGRISPLKVTTSGAFPAPLDQLQVQQTQLQWAD
                     DGYPVAGFGGDIGGLVQGLNDVIPRYMHDLDTVAQTLVTTVNTIHNNGQGQDALNDVN
                     LDFFDPAGVTAATIKLSSDVDGQPSRIALGAVGGGALDGSIGHQMGAAGGLANSPDAI
                     HRAMIGRLGVEAQAATTRASTQAKFAVEAENQRTSVSGVNLDEEMTNLVMSQRAYESA
                     ARLLSAVDEMLDTLINRTAV"
     gene            complement(9139..9669)
                     /locus_tag="PKY13_02300"
     CDS             complement(9139..9669)
                     /locus_tag="PKY13_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOT78784.1"
                     /translation="MSVMPAAPSGAIPELDDLLGELSRTLWKQRGLIELLQYRLEVQQ
                     LILEALREHRLQTALEEVEAAMEDIRRSERTRDAVVRQCAQRLGLDPSASLGDIRVRA
                     EEPWASMLAEHQDALLTLVAGTERLAATNRELAQRGAKETQSVLDAVTGSVTTNAYGP
                     STGRGLARPTLVDRRA"
     gene            9853..11031
                     /locus_tag="PKY13_02305"
     CDS             9853..11031
                     /locus_tag="PKY13_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOT78785.1"
                     /translation="MRINQNVEALNAWRSLSTTNTEMGKSLEKLSSGFRINRAADDAS
                     GLVISQGLRAQVSGLRQATRNAQDGISVVQTAEGALNEVHSMLNRMRDLAVQAANTGT
                     NDTAARTAANAEVTQLSAEIDRISSSTKFGQQSLLNGSFGVTAAIKTGFNATGTYTVP
                     ATSNFQINVNGVGAKTVNLVAGTYTGAQLASHMQTAIRTAMTSGGTYTQAQADAVTVE
                     STTVGAGFSLSIKANLANTQTFTLTDGVGTPLAAQNLTGAITAASGSGGTFQVGSNAG
                     DTISVALASVSATGLGINALDLVNSASTAITALDSAIASVSTTRGNLGAVQNRFESTI
                     NNLQVTTENLAASESRIRDTDMALEMTQFTRHQVLLQAGTAMLGQANQLPQSVLRLLQ
                     "
     gene            11169..13004
                     /gene="fliD"
                     /locus_tag="PKY13_02310"
     CDS             11169..13004
                     /gene="fliD"
                     /locus_tag="PKY13_02310"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOT78786.1"
                     /translation="MPIDGLVSGLDTESIITQLMAAEKVPVDLMTARKTTAQSALDAY
                     KSLATKLSAITTASAALERSTGWNVRSATVSDTAVATATASDGATVGGMQFTVDRLAT
                     THGVATATSVASANDVISSSGNITITVGSTAHNISVGTGSLSDVATAINAAGIGVKAA
                     MVNTGSGYRLQLTAATSGGAAAFSVTSGLNGATVVTTQGVDAKLTFGTGPGAYDITSA
                     TNTFANVLPGVAITAKAVSTTPVTVDVAADGNALATKVKTLVDAVNSALAEIKTRTAY
                     DPSSKTAASLAGDAATRRTSQSLTRALSDAVSQSALGSPGLAGVSLDRNGNATFDQAK
                     FLDAYGKDPAAVERLFVQGATTTGDVQFLSAGSRALPGPRDVVVTLAATQASQVGMVG
                     QFPLTVPATIRIKMGGTEIVYPASPPASQSDTVAGLQAAIDNAGLALTASVNGGGIKV
                     TSNNYGTGAGFDVDFGSGSYTTATGVDAAGTIDGIAATGVGRQLSIPGSDGTLSGLSV
                     LLTSNATGNVGTVNYEPGLAQRISSAIVAATDSTGGYLVDAQNGRQSRIDTLTSSIDS
                     YNTRLEAREKRLRTQFANLEVVLNKLKQQSSMLTSQLSSGSSSSS"
     gene            13067..13573
                     /locus_tag="PKY13_02315"
     CDS             13067..13573
                     /locus_tag="PKY13_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HOT78787.1"
                     /translation="MRPPAALSNRPTAPSPGLVAPATGLTPPSRRVAAARNRYRTDGT
                     TGMTGGRLLVALYQRLLSDLVGAETAIEANQIESAHLQLVHAQEIVDSLEDALDPTAW
                     DQADRMVSLYAHLRRELIAANVTKNAAAVVRCRRIVEPMAQTWQEALDLTTTTPVPSA
                     VAASGVGS"
     gene            13570..13884
                     /locus_tag="PKY13_02320"
     CDS             13570..13884
                     /locus_tag="PKY13_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78788.1"
                     /translation="MSLDVLDSAGADSSVAGWNDTLRSLEHALQHGDADQVASIAIAV
                     TLGPVPPTMTRRATEVLDAIGRLEDEISSQMDAISRELNRHPSRPRWAAPPAPSQLDC
                     SA"
     gene            14143..14487
                     /locus_tag="PKY13_02325"
     CDS             14143..14487
                     /locus_tag="PKY13_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78789.1"
                     /translation="MIGDLTLDTIHAGLNGLTARRAAAEDAVANSETPGYIARNVSFE
                     GELSRAIAGDHPLTATPTTTYTTDEALPNGNNVRLEREIAGLTETALKQQLLVEAANA
                     KFRLLRTVIVGQ"
     gene            14490..14912
                     /locus_tag="PKY13_02330"
     CDS             14490..14912
                     /locus_tag="PKY13_02330"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOT78790.1"
                     /translation="MSGLFGSLGVAYSGMDTYKTWIDATADNVANVNTVRSGDDRAWQ
                     SKYVIARAVPGTNGGARAGIGDGTSVAGVALSDDEGKLVYEPDHPLANAEGMVRYPAV
                     ELGDQMTNLIIAQRAYQANVTVFERARDAYSRALEIGK"
     gene            14916..15242
                     /gene="fliE"
                     /locus_tag="PKY13_02335"
     CDS             14916..15242
                     /gene="fliE"
                     /locus_tag="PKY13_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOT78791.1"
                     /translation="MALIPPVATNFTQAITPSVAPTAPSAASAAGAAQRSDSFEGAIG
                     NALETLNSQQLHADQMARDAATGKLQNVEEYMLAATEAQLGTQLTVAVRNKAVEAFNE
                     IMRMQV"
     gene            15242..16996
                     /gene="fliF"
                     /locus_tag="PKY13_02340"
     CDS             15242..16996
                     /gene="fliF"
                     /locus_tag="PKY13_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOT78792.1"
                     /translation="MSMTDTTNEAPRGGAAANLAALKDRAGKVSGAFDRKQRTVMAAV
                     FAATIGAVLAFSFLGSRTDWAPLMTNLAADDASAVTKQLDSLGTEYRLADGGATVEVP
                     SDVVYQTRIDIASVDMPSSGKVGYGILDNQDITSSEFSQRIGFQRAMEGELAKTIEAI
                     DGVNVATVHLAIPKNDTFALDEQKATASVMVKTAPGKTLAGRQVQAIVNLVSGGIEGL
                     SPTDVTVADGEGNVLAAPGTAPGSGAGGDAAEMTSHYETTLASSIEDMLGSVVGPGKA
                     KVTVAAALDFDSTSSTTETYSAPTTIVAGEALAVNESIKTETYSGDGTGSGAAGILGP
                     DGLPLTSSTQGAAGAAGSGYQLNEKQVNNAVNKVVENTNKAPGAVERLSVAVIVDEKA
                     VDATQLPEIERLVTAAAGLDTSATGRADTIAVSRFKLDTTTQDQVAKELAFRDKQAAA
                     AAKAGALPMPIIAGVGGLLLLLAIVFFLRSRKKKKRAQQLDLAPLELGTGSSALAEQV
                     ITIDATPRRTATVDDPTAVLAGVGAGGIGTGATTGTTSADPGVERREVLGDLIDNQPD
                     EVAQLLRSWLGDRREVTR"
     gene            16993..18021
                     /gene="fliG"
                     /locus_tag="PKY13_02345"
     CDS             16993..18021
                     /gene="fliG"
                     /locus_tag="PKY13_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOT78793.1"
                     /translation="MTAGASSAIAEVGEDMPGVRKVAVLLMSLDTDRAAKLIRELDPD
                     DVAAVAVELEQMQHLGGTTIDTVIEDFARTAAEQRRDVRGGSDYVRRLLEISLGADGA
                     AEVYDQLGGRERFGFLATFDIPTLVTMLSDEHPQTIAVVLAHLAPDRAARILGGFEEP
                     VQCEIGIRVATMERTNAAAVDAIEEALRERADALLAQQTEGGVGGVDSLIAMLTRSDK
                     ATERAVLAELEQIDPEMAEEVRAKLFTFEDIVSLDDRAVQQILRQVDTRGLAVALKGV
                     EDVVRNKVLGNMSSRASENLLEEIDMLKGVRAADVKEARTDIVKVIRTLEESGDIVIN
                     RGIDELLD"
     gene            18014..18670
                     /locus_tag="PKY13_02350"
     CDS             18014..18670
                     /locus_tag="PKY13_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOT78794.1"
                     /translation="MTDPFPSLEQLPTIRSVASGPLAPDVEAAARLAADDGYAEGLRR
                     GYDDGRLAAIADAQADLSFALTALHGAIEDLHHRDVAGVATMAEETVGLALAIAEVVL
                     GREIDTAIDPGRDALVRALAMAPDRGDAVVRFHPEDLARLGDVTTLTGGRRLELVADQ
                     RVERGGCIVDVGPARVDAQLAAALDRVRAELSTAELVRTDHELDAIRHDHLHHDGVVS
                     "
     gene            18667..20031
                     /locus_tag="PKY13_02355"
     CDS             18667..20031
                     /locus_tag="PKY13_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOT78795.1"
                     /translation="MTTLLDNPLVRRALDSGRPEVTGRVVRVVGLTFEIEGLPAAIGD
                     MVRLGDGQHDGVLGEIVALGEHGPIGMPLGDMRGLHIGTSARLDVAAPGVPIGTELLG
                     RVLDGMGNPIDGGPSIDRLHRVGIDGTPPHPLKRQLVRVPQHLGVRALDTLVPCGRGQ
                     RLGVFAGSGVGKSSLLSMIIRGTDADISVLALIGERGREVREFIEHDLGPEGLARSVV
                     IVATSDQPALVRLRAAFVATRIAEWFRDGGANVNLLMDSLTRFAMAQREIGLAAGEVP
                     ATRGYPPSMFGLLPKLLERAGADEHGSITGIYTVLVEGDDMNDPVGDTARSILDGHIT
                     LSRALASQGHFPTIDVLDSVSRVSGAVTTPEQQRMARELRALLAAHKEARDLIEIGAY
                     RPGTNPTVDRAVALRDRIEAFLRQGMNETSAPGVAWNQLAELLATDTFTAEMAGAQTG
                     GGVL"
     gene            20028..20465
                     /gene="fliJ"
                     /locus_tag="PKY13_02360"
     CDS             20028..20465
                     /gene="fliJ"
                     /locus_tag="PKY13_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOT78796.1"
                     /translation="MKKYKFAFGSVQRVQRIAEEQAAVSLAEAQRAADAASAQLQARL
                     ADIGAARPAPGPRSTTEFHAQREHLDRHRVAVTAARAAEINALELLGSARADWVDAAR
                     KVRALDRLDERKRAEWTLETTHAAQLVTDEIATIRYKTEQDRT"
     gene            20465..21619
                     /locus_tag="PKY13_02365"
     CDS             20465..21619
                     /locus_tag="PKY13_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOT78797.1"
                     /translation="MSSIATLAGAHSRIAEIQSRFAPSSVRATTATRTIDTASFDDVM
                     SNLLGSTATDTEGSTIDLTSLTSVLSSLPTRRSGALASALGTTTGTSGNGPTGADAVA
                     AAKQYLGVDYVWGGTDPSKGLDCSGLVQRAYRDLGIELPRVSYDQAKVGTKVDSIEQA
                     RPGDLVAFGSPVDHIGIYVGDGKMIQAPHTGDVVRISDIKRPITAIRRVIPDDAASAS
                     GINATSLAAVTGRTTTTGGSTGIAGAEPYERLFQAAGAKYGLNPRLLAAVAKAESNFD
                     PSARSGAGAVGLMQFMPATAAGMGIDPTDPKQAVDGAAKYLRTQLDRFGSVDLALAAY
                     NAGPGAVQRAGGIPPYAETRNYVTKILGLLGNQSPTTSPTLVGSTNGVLT"
     gene            21616..>22263
                     /locus_tag="PKY13_02370"
     CDS             21616..>22263
                     /locus_tag="PKY13_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78798.1"
                     /translation="MSTDLLPVQSRPAAPAGSTADQRAGAGDAARAASEFDRAVDHAA
                     DKLAGRQGGKHSTSHQVTRHDRPNRLRDGREGQDGDSGETGEATAAGTLPTTEAAEPT
                     TATDTAAAATTDPTSPAPVVVSVPTGAAAVAALLAASDAALATPDLSVTTPTTDTGDA
                     VATVDGGSDTPAATSAGDTEPTPTETNGTTAVSAAPAAADTDTTTAVAVTGTATGS"
BASE COUNT         3686 a         7881 c         7405 g         3191 t
ORIGIN      
        1 gctcgggctc gggctcgggg atgaccgcct ccggctctgg ttcgggctcg acgggttcgg
       61 acggcacaag atccggcgac accggctcgg gggtcgtcgg ctccgagacg tcagactccg
      121 agacgtcaga ctccgccgac gatgctgctt cgttggtcac aggtccagtc tgccgcacag
      181 cgacccggac gcgactgccc gattcgcgca agtgaggcag ccgagcccac gatgtcgtcg
      241 aacggcccga cgacgaggcg tcaggccgag gggcggggcg ggcgcgggag gcgcggcaga
      301 tcggtcgttg ccgtgcccga tgcggcctgg ttggcccggg ccagctcggc gtggatctct
      361 tcgcggtgga ctgtgatctc acggggcgcg tcgatgccga tgcgcacgtg atcgccacgg
      421 atctccagca ccgtcacggt gatgttgccg ttgatgacga tcgattcgtt ggagcgccgc
      481 gagaggacca gcatctacga accgctcaac gggacccggg tggaccaccg cgcctcggac
      541 aggaccactt ggcgggcacg acgggtacga gtgttcacca cgaggggccc gagcaggttg
      601 gccgtgatgt cgtcgtcgga gatggtgacc aggcagagga gcaacgtctc cgactcgtcg
      661 cggaggtcga gttcctcgag atccttgtcg ttgacctcgg gcgcgtagtc gtcgaagaag
      721 aagtgcggtg ccgtcacgag gaaggcgaga cccggatcac tcgtcgactg gagccagctg
      781 tacacaccgt cgtcaccgac gggaacgatc acgaacgagg actggtcttc gaaccccaac
      841 aacccgtgcg ggaaggtgag ggtcgaggat tcttcgcagt caacgtcgcc gaagcgctcg
      901 gattggatga gcacactcat gaccttaccg acccgtcacg ccggagcggt cgaccgtcgc
      961 gccactcgtc gcccgtccgg ccagaacggt ggcggcccag agctcgggag tggcgggacg
     1021 accatcgatg gaggcgacgg gcgtcgggag ctggagcgca tcgtcgggcg ccgggtgctc
     1081 ggccgcaccg agcagatcga tcacgtcgac cccaccgacg gcggcgatac tcaggtgctg
     1141 atgggcgatc agccgcgtcg tatcggcggc gaggcgcacc tgctcggcgc gaagaccgtc
     1201 gagcacgacc cggacccact cgtgtccctg tcggcccggc accagctcca gcacgatgac
     1261 cgcgacgggg gcgcagcgat accgcgtggt cgccgccaac cgcatggcgt cctccggcgt
     1321 cgggacgacg acatcggccg gcggcgacgc cacgatgacg tcatcggtcg ggatgccgag
     1381 tcgggcggcg agcccaccgg cgacctcgag cgcctcggtg cgtgaaccga tgaccgcgac
     1441 gattcccgct gtcggcagcg gcggcgccgg cggaacggtc tcgaagcgtg cgatgagacc
     1501 gttcaggtcg acgcgggcgc cggcgcggat cgtgccggtc gagcccgcgg cctccgccga
     1561 gacgggaagg gtggtggtga ccggcagacg cactgccgcc ggggccacac cggggaacgg
     1621 cggagtgacc tgcgccgggt cgaacggcgt gccgaccacg ctcgatgccg ggatgagcgt
     1681 cgtcgacgcg atcggcccgg ccgagtggat gggagcaacg atctcgggct gctgcgcgac
     1741 gggagcgacg acagcggggg cgatcgttcc ggccgccggg actggaaccg ggacaggaac
     1801 cggcgcggga accgcaaccg gaacgggcag cggaacgggc accgaggcct gcacctgggg
     1861 cgctgccggg gccggcggtg tcgacacggc cgccttggcg agcaacgccc agatctcggc
     1921 gtcggcgtcg tcacgaacgg gtgccggctc gagcgacaag gcaccggcga catcctcggg
     1981 gtcgatctcg tcgtagtccg gctcgtcacc gatcacttcg aggtcgctga cggcgtcatc
     2041 gccgaagatg ctcatgcgac ccaacggcac gaagttcagc tcggcgtcgc catcgtcggc
     2101 gggagcgggc accgtgaccg tcacttggaa cagctccttc gcgaagaagc cgccgatgcc
     2161 gccgcggcgg acccggtcgg cagcagtgat ggtcacgtcg gcaccgtgcg cggcgtgcac
     2221 gcgggcgagc acggcctcga gcgggaagcc ctcgtaccgc agctcgcgcg agccggtgcc
     2281 ggctaccggc cggcggtcaa gcattgctct ggtcaagggt caccgctcct agatcatcga
     2341 tggtgagatg gtcggccaac tcgtcatagg acagcaccgg aaggtgcgga acgagtcggc
     2401 tcacgaaatt gcgcatcgcc gggcgcatgc tcggggcgca gacgagcacc ggctcgtggc
     2461 cctcctgctc agcggcacgg agctgcgtga cgagcgcccg gccgatgcgc gccgcggtct
     2521 ccggttcgag cgcgaggtac gacccggttt cgttgcgctg caacgtgccg agcagtccct
     2581 gctcgaccag cgggtcgagc gtgatcaccg cgagatgccc gttgttggag cgggcggcgc
     2641 agatggtggg gccgagcgac tgacggaccg cctcgacgac gagttcggtg tcacgggtga
     2701 cgcgggcgcg ctcgctgatc acctcgagga tgcgggtgag atcgcggatc ggcacctgct
     2761 cggccagcag gtggtggagc acctgctggg tctcggcgag gctcacacca cacgcggcca
     2821 ggtcctcgac gactgcgctg tcgtgctcgc gaacgagatc gatgagtgcc ttcacgtcct
     2881 ggcgactcag gagctgcggc gcgtgttccc gggcgatctc ggcgagatgc gtcgtgacga
     2941 ccgacgcccg gtcgacgacc gtcccgccca tggcctcggc ggaggcccgg tgctcggggc
     3001 cgatccactt ggccggcaga ccgaagaccg gttcgaagac ctctgggccc gggtagatcg
     3061 agaggtcgtc ggcgatcacg aggaagcgac cgttcggcgc gtgcccggtc gcgacttcca
     3121 cgccgtgaat gcgaacgagg tacgtgccga gctcgagctc cacgttgtcc cgggtccgaa
     3181 ccggcgggat cacgatgccc aattcctcga cgagctttcg tcgcagggcc tgcacgcggt
     3241 cgagcaggtc gccgttggcg gccgggtcga cgaggtcgac gaggtcgacg gcgagctcga
     3301 ggctgagcgg ctcgatgcgg agggcacgcc ccagctcggc cggcgagttc gggtcgtcgt
     3361 cctcctcgtt caccggtggg agctcgagca tctgctgttc gagcgcctcg gccgccttcc
     3421 cgagcctgcg accgagcagg taggcaccac cgccgaccat gaggaacggg atcttgggaa
     3481 tgcccggggc aacggcgaag gccatcatcg ccagaccgcc ggttcggatg gcgcggtgct
     3541 gacgggcgaa ctgtgaggtg acgtcgctgc ccagatcgtc cgcgctggcg gcccgggtga
     3601 tgatgaggcc cgaggagatc gacacgagga gcgccgggat ctgggagatg agaccgtcgc
     3661 cgacggtgag gaggctgtag gtctggacgg cgtcgccgat cggctgacct cgctggatca
     3721 cgcccacgag caggccaccg atgaggttga ccgcggtgat cacgatcgcg gcgatcgcat
     3781 cgcccttcac gaacttggac gcaccgtcca tcgcaccgta gaagtcggcc tcggcggtga
     3841 cctcgttgcg gcgacgacgc gcctcgatgt cgtcgatcac cccggcgttg aggtcggcgt
     3901 cgatcgccat ctgcttgccg ggcatcgcgt ccaaggtgaa ccgggccgcg acctcggcca
     3961 cgcgggtcgc accgttggtg atcacgacga actggatgac gaccaggatg aagaagacca
     4021 cgaggccgac gacgagcgat ccgccgatga cgaacgatcc gaaggccttg atgacctcgc
     4081 cggcatcgcc ctccgacagg atcaggcgcg ccgtgctgac gttgagaccc aaccggaaca
     4141 gcgtcgcaat caggagcagc gacgggaagg acgccagatc gagcgccttc gaaacattca
     4201 gcgatgccag gagcatgagg atcgccagcc cgaggttgaa tgcgagcaac acgtcgagca
     4261 cgacggtcgg gatcgggatg accatgaggg ccaccacgaa caccacggcc atcgggaaga
     4321 ggctcgcgcc gagcttcttt ggcattacga ggactccttg acggtcacgg atctcgtcgt
     4381 tcgaacacgc ccgggggcgg gaacgacgcg gcatccatgc cggtgagggg tggccttcgt
     4441 cctgattggc tctgcttcca tcggcagctc acgcgagaag ttgagcaata tcacccaacc
     4501 gccgcctcgc ggcgcggtcc gccggcgtcc gcggcggccg ctgcctcctc ggcctcccgc
     4561 ttctcgagca tccgacgacg cgccggcggc acccgcacca gctccgaacc gctcggcgtc
     4621 cacgtgtcgg gcacctccag gccgatgggc ccgccgaacg tccgccgggt cgcgctgagc
     4681 tggtggagga acgcgaggac cgtcgcgacg ccctggtaga gctccggcgg gatctcctcg
     4741 cccacccggc acacggcgta gagcgcacgg gcgagcggtt tggcttccac gagcggcacc
     4801 ccggcggccg caccctcggc tttgatcttc tgggcgagcc cgtcggcgcc acgggcgatc
     4861 accttcgggg ctccgagcgc ccgttggtac tcgatcgcga tggcgaagtg agtcgggttc
     4921 gtgatgatga cgtccgcgct cgggatcgcc gcgagcatgc ggttgcgcga catctggttc
     4981 tgcatctgac ggcgcttgcc cttcatgtgg gggtcgccct cggactcacg agcctcctgc
     5041 ttgatctcgt gcttgctcat cttgaggtcc ttcgcctgtt tgcggcgttg gaacgcgtag
     5101 tcggcgagcc cgacgacgag cgccgcggcg gcgatcgccc tcaccagggc cagcgccgac
     5161 atcgcggtgg cgtggacggc ctgcgaggcg ttgcggaacg gcgcccgcac gatgtcggcc
     5221 gcagtgccgc ggaccatcac ccacgacacg gccaggatga tgccgagctt catcgtcgcc
     5281 gtgagggtcg accagagact ctgcaccgag aagatgcgct tgatcccctt gaaggggctg
     5341 agccgcgaca tctggggctt gagcggcttc cccgtgagga gcagcccgac ctgcgcgagg
     5401 ttgccgagga tcgcgatcac gagcagagcg agcattgccg gcaggatcgc accgagtgtc
     5461 gccaccatcg ctcggcccac gtacgtgggc agcatcttgg cgtcggggtt gtgcgccagc
     5521 tcgacggtgg atcgcaacac cgcggtgacc gcagagccgg tgcggctgac catgccgggc
     5581 atcacgatgg tgccgatgag caccgaggtc caggtgacga gatcctcgga gcggacgacc
     5641 tgtccgtcgc gccgcgcctc cttcttccgt ttggctgtcg gggcctcggt cttgtcgtgc
     5701 ttgtcaccgg acatcgctcc tcatcacccc cctttgatca gggcgtgcat caccgacatg
     5761 gcttccttga ggagctgtgc cgacacgtag gggatgaggg gcagggcggc gccgagcagc
     5821 atgaacagca ccaggctctt cgcgccgaac cccagcacca tgatgttgag cttcggcgcg
     5881 gcgcgactcg cgagacccaa caggagctcg gtcacgaaga gcgctccgag gatgggcgcg
     5941 gacacctgca cggccgcgat gaggaaggcc tgcacgtcgt gggtgatcaa cgttccgacc
     6001 cggtcgagcc ggaacccgct caccggcgac agttcgaacg tgcgcgacag ccccccgatc
     6061 agcatcaagt ggccgttggt cacgaacaac acggcggtgc cgagtgtgct gtagagccgc
     6121 ccgaccgggc cggtggtggt cttggtcatg gggtcgaaca gctgcgccga ggtgaaggcg
     6181 gcgaacgcat cgatcatgcc gccgccggcg gcgatcgcct ggaagagcac gtagacgagg
     6241 aatccgagca ccaggccgat cacggcgttg tgcagcgcac cggtgacgag ggaccaggtg
     6301 tcggtcgaca ggggcgtgtc gccctgcatg aggcgcgggg ccatggccag gccgagaccc
     6361 atggacagtc cggtccggat ccggaccgga accgaaccct ggaacggcgg cgcgatggtc
     6421 agccacgcac cacaccggat ggtcgcgaac atgaacgcca cgaggaactc ggggttgagg
     6481 gagagcctca tgggcgcggg gttcgggcct gggccgaatg gaccatgcgc tcctatcggc
     6541 gcgagacggc agaacctgag ccggtcgttg gtcggatcgc cgaggtaccg gagcgaagcg
     6601 gaggtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcct cggcccatca
     6721 ggacagcatc gttgtgtcgg cgaagccgac agcacccgag tcaccgacgt gagtcagcgc
     6781 aagaagtcca tcaggctcgg ctggatgacc tttgccgtcg cctggatcgc cgcctgatag
     6841 gcggcctcac gggtcttcat gttgatgacg gcctcggcga agtcgatgtt ctccttgttg
     6901 gagatacccg acttgaggga gaccttgctg tcctcgagcg agttggtgag gtcctcgagc
     6961 tgggacgccc ggctgccgag ctgcacctgc gccgtcgcga cgcgcttggt ggcggcgtcg
     7021 acctggccga gccccgcgct gatcgcagcg gtgtcgccgg tctccacggc ggtcgccagc
     7081 gcacccaaca gctggaagag gtcaccctgg gtgggatcgc ccgggttgtt cacgccgaag
     7141 atctcggggc cggtccggtt gacctgcaag gtgaccccac cggtgatggg gaggttcacc
     7201 gcgccgttgt cgccgaggta cacaccgttg gcgtcatagg ccgctgcgcc ggcggcgttg
     7261 cccgcgaaca aggggcgacc cccacgctcg gcgttcgatg ccagcaggat cgactcgcgt
     7321 tgggcccgga tctggttggc caaggcggcc cgcgaggtca cgtcgttcgc tgccgagttc
     7381 gcctgcacca gcgtggcccg aaccccggcg agacgatcac tgacgtcggc gagggccgcg
     7441 tcaccggcat cgaaccagga ggtcgcctcc tcggcgttgc gtgagtactg ctcgagccgc
     7501 cgaagcgtcg ctcgctgatt gagcgagctg acggcgtcgg cggggttgtc gctcacccgg
     7561 ttgatgcgac gaccggtggt cacctgctcc tgggcgcgga acagttcgct gttggcgttc
     7621 tggatgctct gcagcatcgt gcgggcgccg gagagactgg cgattcgagt gatcatgacg
     7681 cttccttgcg tgtgatccgg gctcgtccga tcatcgctgt gatcagacgg ccgtgcggtt
     7741 gatgagcgtg tcgagcatct cgtcgacggc ggacaggagc cgggcggccg attcgtaggc
     7801 gcgctggctc atgacgaggt tggtcatctc ctcgtcgagg ttcacaccgc tgaccgacgt
     7861 gcgctggttc tcggcctcga cggcgaactt cgcctgggtg gacgcccggg tcgttgctgc
     7921 ctgcgcctcg acgccgagtc ggccgatcat ggcgcggtgg atcgcgtcgg gcgagttggc
     7981 gagtccaccg gcggcaccca tctggtggcc gatcgagccg tcgagcgctc cgccgccaac
     8041 ggcgccgagg gcgatgcgcg acggctgacc gtcgacgtcg gacgacagtt tgatcgtggc
     8101 agcggtgacc cccgccggat cgaagaagtc gaggttgacg tcgttgaggg cgtcctggcc
     8161 ctgaccgttg ttgtggatcg tgttgacggt ggtgaccagg gtctgcgcga cggtgtcgag
     8221 gtcgtgcatg taccgcggga tcacgtcgtt gaggccctgc accagaccgc cgatgtcgcc
     8281 gccgaatccg gcgaccgggt agccgtcgtc ggcccactgg agctgcgtct gctgcacttg
     8341 gagctggtcg agcggcgccg ggaacgcgcc cgacgtcgtg accttgagcg gggagatgcg
     8401 cccggccgac acgagcgagc tcccgcccag catcacgttg acctggtcgt actcgatggg
     8461 ccgaacctcg gctccggtga gcgacgtcag ttggtcgacc aggcggtcgc gctcgtcggc
     8521 gagatcgccg gtgtccatac cggcgacgct cgccgcccgg atctgcacgt tgaggtcggc
     8581 gacctgttgc gccagcgtgt tgacccgatc cacccccatc gtgagctgcg acgagaggtc
     8641 ggtgtgttgg gtctgaaggc tgccggcagc ctggttcagc gacgcggcca ggttcgacgc
     8701 ctgggcgagc accgctgcac gctgcgggat actgccgggc tggttggcgg catcggagaa
     8761 cgcgttccag aaggccgaca gctgaccggc gatcgcggtg tcgctcggct cggggaacgt
     8821 gccctcgaca ccaccgagga ccgaatcgag acgagcggcg gaagcggagc tggcgaggct
     8881 ggcccgggct ttcacgtcga ggaactgatc acggatccgg ttgacgccgg cgatgttcac
     8941 gccgaggttg ttccacgaga agtcggtgtc gaaccgcgac gcgatcttca tgcccgtcgc
     9001 cggttcgagc agcacctgac gacggctgta gccctcggtg ttgacgttgc tgatgttgtg
     9061 cccgatgacg tcgatgacgc gcttgtgcgc ctgcagcccc gacagggcgg tgttgagggt
     9121 cgagaaactg ctcatcgatc aggcccgacg atcgacgagg gtcggacggg caagaccacg
     9181 gcccgtcgac ggtccgtagg cgttggtcgt gaccgatccg gtcacagcgt cgaggaccga
     9241 ctgtgtctcc ttcgcgcccc gctgggcgag ctcccggttg gtcgccgcca ggcgttcggt
     9301 gccggccacg agggtgagca acgcgtcctg gtgctcggcg agcatcgacg cccacggctc
     9361 ctcggcgcga acccggatgt cgccgagcga cgccgacggg tcgagaccca accgctgggc
     9421 gcactggcgc acgacggcgt cacgggtgcg ctcgctgcga cggatgtcct ccatcgccgc
     9481 ctcgacctct tcgagggcgg tctgcagtcg gtgctcgcgc aacgcctcga ggatcagctg
     9541 ctgcacctcc aaccggtact gcaggagctc gatgagcccg cgctgcttcc agagcgtccg
     9601 cgacagctca ccgagcaggt cgtcgagttc gggaatagcg ccggaggggg cagcgggcat
     9661 gacactcatg gactccttca tcggcacatc gcgccagaac ctgaggatcc gagcgaggtt
     9721 cgggatccga gcagaaaatt gctcaaggaa tctccgccga cgtcgataac tacgtggtca
     9781 cccacctcgg gtggagtggc tcgtgacccc aggacgggat cgcgatcaag cattcatcac
     9841 ggaggatttc caatgcgtat caaccagaat gtcgaagcgc tcaacgcctg gcgcagcctc
     9901 tcgaccacca acaccgagat gggcaagtcc ctcgagaagc tctcctcggg tttccgcatc
     9961 aaccgtgcgg ccgacgatgc ttcgggcctc gtcatctccc agggcctgcg tgcccaggtg
    10021 tccggccttc gtcaggccac ccgcaacgct caggacggca tctctgtcgt gcagaccgct
    10081 gaaggtgcgc tcaacgaggt ccactccatg ctcaaccgca tgcgtgacct tgccgtccag
    10141 gctgccaaca ccggcaccaa cgacacggcg gcccgtacgg cagccaacgc cgaggtcacc
    10201 caactgtcgg ctgagatcga ccgcatctcg agctcgacga agttcggtca gcagtccctg
    10261 ctgaacggct ccttcggcgt gacggctgcg atcaagaccg gcttcaacgc caccggtacc
    10321 tacaccgtcc cggcgacgtc gaacttccag atcaacgtca acggcgtcgg cgccaagacg
    10381 gtgaacctgg tcgccggcac ctacaccggt gcccagctgg cctcgcacat gcagaccgcc
    10441 atccgcacgg caatgacctc gggtggtacc tacacccagg cgcaggccga tgcggtgacc
    10501 gtcgagtcga ccaccgtcgg cgccggcttc tcgctctcga tcaaggcgaa cctggccaac
    10561 acccagacgt tcaccctgac cgacggtgtg ggcacgccgc tggcggcgca gaacctcacc
    10621 ggtgcgatca ctgctgcctc gggcagcggc ggcacgttcc aggtcggctc caacgccggc
    10681 gacaccatca gcgttgcgct cgccagcgtc agcgccacgg gcctgggcat caacgccctg
    10741 gacctggtga actcggcatc gacggcgatc accgccctcg actcggccat cgccagcgtg
    10801 tcgaccaccc gaggcaacct cggtgccgtt cagaaccgct tcgagtccac catcaacaac
    10861 ctccaggtca ccaccgagaa cctggccgct tcggagtccc gcatccgtga caccgacatg
    10921 gccctcgaga tgacgcagtt cacgcgtcac caggtgctcc tgcaggccgg cacggccatg
    10981 ctcggccagg ccaaccagct tccgcagtcg gttctccgtc tgctccagta gtcgggaatc
    11041 cccttcgggg gacccactcc gtgtgaacgg tctgggagca gggcaactcg tcagaggtcg
    11101 ccctgctccc ggcgccgtcc catccggacc cgacgctgga cgtcgggagt caccaggagg
    11161 tgaccaccat gccgatcgat ggactggtca gcggtctcga taccgaatcc atcatcaccc
    11221 aactgatggc agccgagaag gtgcccgtcg atctgatgac ggcccgcaag acgaccgcgc
    11281 agtcggcgct cgacgcctac aagagcctcg ccacgaagct cagtgccatc acgaccgcga
    11341 gcgccgcgct cgagcgctcc accggatgga acgtccgcag cgcgaccgtc tccgacacgg
    11401 ccgtcgccac cgcaactgcg agcgacggcg caacggtcgg cgggatgcag ttcaccgtgg
    11461 accgcctcgc gacgacccac ggcgtggcca ccgccacgag cgtcgccagt gccaacgacg
    11521 tcatctcgtc gtcgggcaac atcacgatca cggtcggttc gaccgcccac aacatctcgg
    11581 tcgggaccgg ctcgctcagc gacgtcgcca cggccatcaa cgccgccggg atcggggtga
    11641 aggccgcgat ggtcaacacc ggctcggggt atcgcctcca gctcaccgcc gccacgagcg
    11701 gcggtgccgc cgcgttctcg gtcacgtccg gcctcaatgg cgccaccgtc gtcaccacgc
    11761 agggcgtcga cgcgaaactc acgttcggca ccggtccggg ggcctacgac atcacctcgg
    11821 cgacgaacac gttcgccaac gtgctgcccg gtgtcgccat cacggcgaag gccgtcagca
    11881 cgacccccgt cacggtcgac gtcgcggccg acggcaacgc gctggccacc aaggtcaaga
    11941 cgctcgtcga cgccgtcaac agcgccctcg ccgagatcaa gacccgcacg gcgtacgacc
    12001 ccagctcgaa gaccgcagcc tcgctcgccg gcgacgccgc aactcgacgg accagccagt
    12061 ccctcacccg ggcgctgagc gacgcggtga gccagtccgc gctcggatca cccggcctcg
    12121 ccggtgtgtc gctcgaccgc aacggcaacg cgacgttcga ccaggccaag ttcctcgacg
    12181 cctacggcaa ggacccggcc gcggtcgagc gcctcttcgt ccagggcgca acgacgacgg
    12241 gcgacgtcca gttcctctcg gccggttccc gggcgctccc tgggccccgc gacgtcgtgg
    12301 tcacgctcgc cgcgacccag gccagtcagg tcggcatggt cggccagttc ccgctcaccg
    12361 tcccggccac catccgcatc aagatgggcg gcaccgagat cgtctatccg gcgtcgccgc
    12421 ccgcatccca gtccgacacc gtcgccggac tgcaggccgc gatcgacaac gccggcctcg
    12481 cgctgacggc gtcggtcaac ggcggtggca tcaaggtcac gtccaacaac tacggcaccg
    12541 gagcggggtt cgacgtcgac ttcgggtcgg gcagctacac cactgccacc ggcgtggacg
    12601 ccgcgggaac catcgacggc atcgccgcga ccggggtagg acgccagttg tccatccccg
    12661 gctccgacgg caccctcagc ggcctgtcgg tcctgctcac cagcaacgcg acgggcaacg
    12721 tcggcacagt caactacgag cccggtctcg cccaacgcat ctcgagcgcc atcgtcgccg
    12781 ccaccgattc gacgggcggc tacctcgtcg atgcgcagaa cggccggcag tcgaggatcg
    12841 acacgctcac atcgtcgatc gacagctaca acacccggct cgaagcccgg gagaagcggc
    12901 tgcgcacgca gttcgccaac ctcgaggtcg tcctcaacaa gctcaagcag cagagctcga
    12961 tgctcacctc gcagctcagc tccggctcgt ccagcagtag ctgaccgagt tcgcacgtcc
    13021 gaatccacca tctgttcgcc cggctactgc aaacgaaaag gacttcatgc gaccgcccgc
    13081 agcgctctcc aaccgtccca ctgcaccgtc ccccggcctc gtcgccccgg cgaccgggct
    13141 cacgccgccg agtcggcgcg tcgcagcggc acgaaaccgc taccggaccg acggcaccac
    13201 cggcatgacc ggtggccgtc tgctcgtcgc cctctaccag cggctcctgt cggacctggt
    13261 cggcgccgag acggccatcg aggccaacca gatcgagagc gcccacctcc agctcgtgca
    13321 cgcccaggag atcgtcgact ccctcgagga tgcgctcgat ccgacggcgt gggaccaggc
    13381 cgaccgaatg gtttcgttgt acgcgcacct ccgtcgcgag ttgatcgccg cgaacgtcac
    13441 caagaacgcc gcggcggtgg tccgctgccg acgcatcgtc gagccgatgg cccagacctg
    13501 gcaggaggcg ctcgacctca cgacgacgac gccggtgccg tcggccgtgg ctgcgtcggg
    13561 agtggggtcg tgagcctcga cgtcctcgac agcgccggtg ccgactcctc ggtcgcgggg
    13621 tggaacgaca cgctgaggtc actcgagcac gccctgcaac acggcgatgc cgaccaggtc
    13681 gcctcgatcg ccatcgccgt cacgctcggc ccggtcccgc cgacgatgac gcgtcgagcg
    13741 accgaggtgc tcgacgcgat cggacgactc gaggacgaga tctcgagcca gatggacgcg
    13801 atctcccgcg agctgaaccg gcatccgtcg cgcccgcggt gggcggcgcc gccggctccg
    13861 agccaactcg actgcagcgc ctgaccagcg gttcgctccc gcgaacccct gagaattagg
    13921 tcgtccgcct gaagtcggtg ggccgtctgg cacccccaat ggggccaaac ggcgccattt
    13981 gcagtgttga tgtctgagat ctgggcgaat ttgctcaggg tcgctgggca aatgccgata
    14041 gagaccggag cacggagagc tcactcttcc gccagggatc ggcgtcgcaa caggctctga
    14101 gggagcacga atgcccgcgc catctctccc gtcgatcaac tcatgatcgg cgatctcaca
    14161 ctcgacacca tccacgccgg tctcaacggg ctcaccgccc gccgcgccgc tgccgaggat
    14221 gccgtcgcga actccgagac gcccggctac atcgcccgca acgtgtcgtt cgagggcgaa
    14281 ctgagccgcg ccatcgccgg tgaccacccg ctgaccgcga cgcccaccac cacctacacc
    14341 accgacgagg cgcttccgaa cggcaacaac gtccgcctgg aacgcgagat cgccggcctg
    14401 accgagacgg cgctcaagca acaactgctc gtcgaggccg ccaacgcgaa gttccggttg
    14461 ctccgcaccg tgatcgtggg tcagtagcca tgagcggact cttcggatct ctcggcgtcg
    14521 cctactccgg catggacacg tacaagacgt ggatcgatgc cacggcggac aacgtcgcca
    14581 acgtcaacac cgtccgcagc ggcgacgacc gcgcgtggca gtccaagtac gtgatcgccc
    14641 gcgccgtgcc cggcaccaac ggcggtgccc gcgccgggat cggcgacggg acgtccgtcg
    14701 ccggagtcgc cctcagcgac gacgagggca agctcgtcta cgagcccgac cacccgctgg
    14761 ccaacgccga ggggatggtg cgctaccccg ccgtcgaact cggtgaccag atgacgaacc
    14821 tgatcatcgc ccagcgcgcc taccaggcca acgtcaccgt cttcgagcgg gcccgcgacg
    14881 cctactcccg tgcactcgag atcggaaagt agcccgtggc cctgattccc ccggtcgcaa
    14941 ccaacttcac ccaggcgatc actccgtcgg ttgccccgac agcacccagc gccgccagcg
    15001 ccgccggagc agcacagcgt tccgattcgt tcgaaggcgc catcggcaat gccctggaga
    15061 cgctgaacag ccagcagctc cacgccgacc agatggcccg cgacgcggcg accggaaagc
    15121 tccagaacgt cgaggagtac atgctcgccg ccaccgaggc gcaactcggc acccagctca
    15181 ccgtcgctgt tcgcaacaag gccgttgagg ccttcaacga gatcatgagg atgcaggtct
    15241 gatgtcgatg accgacacca ccaacgaggc cccgcgcggc ggtgccgccg ccaacctggc
    15301 agcactgaag gatcgcgccg ggaaggtgag cggcgccttc gaccgcaagc agcgcacggt
    15361 catggccgcc gtgttcgccg cgaccatcgg ggccgtcctg gcgttctcgt tcctcggcag
    15421 ccgcaccgac tgggcaccgc tcatgacgaa cctggctgcc gacgacgcct cggccgtcac
    15481 gaagcagctc gactcactcg gcaccgagta ccgcctcgcc gacggcggcg ccaccgtcga
    15541 ggtcccgagc gacgtcgtct accagacccg tatcgacatc gcctcggtcg acatgccgtc
    15601 ctcgggcaag gtcggctacg gcatcttgga caaccaggac atcaccagct cggagttcag
    15661 ccagcgcatc ggcttccagc gggcgatgga gggtgagctc gccaagacca tcgaggcgat
    15721 cgacggcgtc aacgtggcga ccgttcacct ggccatcccg aagaacgaca cctttgcgct
    15781 cgacgagcag aaggcaacgg cctcggtgat ggtgaagacc gctcccggca agacccttgc
    15841 cggccggcag gtgcaggcga tcgtcaacct cgtctccggc ggcatcgagg ggttgtcgcc
    15901 caccgacgtg accgtcgccg acggcgaggg caacgtgctc gccgctcccg gcaccgctcc
    15961 cggcagcggc gccggcggcg acgcggccga gatgaccagc cactacgaga cgacgctggc
    16021 atcgtccatc gaggacatgc tcggcagcgt ggtcggcccc ggcaaggcca aggtcaccgt
    16081 cgcggcagcg ctcgacttcg actcgacgtc gtcgacgacc gagacctact ccgctcccac
    16141 cacgatcgtc gccggtgaag cgctggcggt gaacgagtcg atcaagaccg agacctacag
    16201 cggtgacggc accggttcgg gagcggccgg catcctcggc cccgacggcc tccccctcac
    16261 cagctcgacc cagggagcgg ccggagcggc cggttctggg taccagctga acgagaagca
    16321 ggtgaacaac gccgtcaaca aggtcgtcga gaacacgaac aaggcgcccg gagccgtgga
    16381 gcggttgtcg gtcgccgtga tcgtcgacga gaaggcggtc gatgccaccc agctgcccga
    16441 gatcgagcgc ctggtcacgg ccgccgccgg actcgacacc agcgccaccg gccgcgccga
    16501 caccatcgcc gtctcacgct tcaagctcga cacgacgacc caggaccagg tcgccaagga
    16561 gctcgccttc cgcgacaagc aggccgcggc ggcagccaag gccggtgccc tcccgatgcc
    16621 gatcatcgcc ggcgtcgggg gcttgctcct cctactcgcc atcgtcttct tcctccgctc
    16681 ccgcaagaag aagaagcggg cgcagcagct cgatctcgca ccactcgagc tgggcaccgg
    16741 cagttccgca ctggcagagc aggtcatcac gatcgatgcg accccgcgcc ggacggccac
    16801 ggtcgacgac ccgacggcgg tactcgccgg tgttggggcc ggcggcatcg ggaccggcgc
    16861 gaccaccggc acgaccagcg ccgaccccgg cgtcgagcgc cgtgaggtcc tcggcgacct
    16921 catcgacaac cagcccgacg aggtggccca gctacttcgt tcgtggctcg gtgaccgccg
    16981 cgaggtgacc cgatgaccgc cggcgcctcg agtgcgatcg cggaggtcgg cgaggacatg
    17041 cccggcgtcc gcaaggtggc tgtgctgctc atgagcctcg acaccgaccg cgccgccaag
    17101 ttgatccgcg agctcgatcc cgacgacgtc gccgccgtcg ccgtcgaact cgagcagatg
    17161 cagcacctgg gcggaacgac catcgacacg gtgatcgagg acttcgcccg cacggccgca
    17221 gaacagcggc gcgacgtgcg cggcgggtcc gactacgttc gccggctgct cgagatcagc
    17281 ctcggagcgg acggtgccgc cgaggtctac gaccagttgg gtggccgcga gcggttcggt
    17341 ttcctcgcca cgttcgacat cccgaccctc gtcacgatgc tctccgacga acatccccag
    17401 acgatcgccg ttgtgctcgc ccacctcgca cccgaccggg cggcgcgcat cctcggcggc
    17461 ttcgaggagc cggtgcagtg cgagatcggc atccgcgtcg cgaccatgga gcgcaccaac
    17521 gctgccgccg tcgacgcgat cgaggaagct ctccgcgaac gcgccgacgc gctgctggcg
    17581 cagcagaccg aaggcggcgt cggcggcgtc gacagcctca tcgccatgct gacccgttcg
    17641 gacaaggcca ccgaacgagc cgtcctcgcc gaactcgagc agatcgaccc cgagatggcc
    17701 gaagaggttc gggcgaagct gttcacgttc gaggacatcg tcagcctcga cgaccgcgct
    17761 gtgcagcaga tcctccgcca ggtcgacacc cgtggtctcg ccgtggcgct caagggggtc
    17821 gaggacgtcg tccgcaacaa ggtgctcggg aacatgagct cgcgtgcgtc ggagaacctc
    17881 ctcgaggaga tcgacatgct caagggagtc cgtgccgccg atgtcaagga agcgcgtacc
    17941 gacatcgtga aggtcatccg cacgctcgag gagagcggcg acatcgtcat caaccggggc
    18001 atcgatgaac tccttgactg acccgttccc gtcgctcgaa caactgccga cgatccggtc
    18061 cgtggcatcg ggcccgctgg cccccgacgt cgaggccgcc gctcggctcg ccgccgacga
    18121 cggctatgcc gaggggttgc gtcgtggcta cgacgacggc cgtttggcag ctatcgccga
    18181 cgcgcaggcc gacctgtcgt tcgcgctgac cgcgctccac ggagcgatcg aggacctgca
    18241 ccatcgcgac gtcgccggcg tggcgaccat ggccgaggag accgtcggtc tcgcgttggc
    18301 catcgccgag gtcgtgctcg gccgcgagat cgacaccgcc atcgatccgg gccgcgacgc
    18361 actcgtccgc gcgctggcga tggcgcccga ccgcggcgat gccgtcgtcc ggttccaccc
    18421 cgaggacctc gcccgcctcg gcgacgtgac caccctcacc ggtgggcgcc gcctcgaact
    18481 cgtcgccgac cagcgcgtgg agcgcggcgg atgcatcgtc gacgtcggcc ccgcccgcgt
    18541 cgacgcccag ctcgccgctg ccctcgaccg tgtgcgcgcc gagctgtcca cggcagaact
    18601 ggtccgcacc gaccacgaac tcgacgccat ccgccacgac catctgcacc acgacggagt
    18661 cgtctcgtga ccaccctcct cgacaacccg ctcgtccgtc gagccctgga ctcgggccgc
    18721 cccgaggtga ccggtcgcgt ggtccgcgtc gtcggtctca cgttcgagat cgaaggcctc
    18781 ccggcggcga tcggtgacat ggtccgcctt ggcgacggcc agcacgacgg tgtgctcggc
    18841 gagatcgtgg cgctcggcga gcacggtccg atcgggatgc cgctcggcga catgcgcggc
    18901 ctccacatcg gcacctccgc ccgcctcgac gtcgcggcgc cgggcgtacc gatcggtacc
    18961 gaactgctcg gtcgggtgct cgacggtatg ggcaacccga tcgacggagg cccctccatc
    19021 gaccgcctcc accgcgtcgg catcgacggc accccgccgc acccgctcaa gcgccagctc
    19081 gtccgcgtgc cccagcacct gggcgtgcga gccctcgaca ccctcgtgcc ctgcggccgg
    19141 gggcaacgcc tcggtgtctt cgccgggtcg ggcgtcggca agtcgtcgct cctctcgatg
    19201 atcatccgcg gcaccgacgc cgacatctcg gtcctcgccc tcatcggcga acgcggccgt
    19261 gaggtgcgcg agttcatcga gcacgacctc ggccccgagg gcctcgctcg ctccgtcgtg
    19321 atcgtggcga cctcggacca gccggcactc gtccgcctcc gggccgcgtt cgtcgcgacc
    19381 cgcatcgccg agtggttccg cgacggcggc gcgaacgtca acctcctgat ggacagcctc
    19441 acccgcttcg cgatggcgca gcgcgagatc ggcctcgcgg ccggcgaggt ccccgccacc
    19501 cgtggctatc cgccgtcgat gttcggcctc ctgccgaagc tcctcgagcg cgccggtgcg
    19561 gacgagcacg ggagcatcac cggcatctac acggtgctcg tcgagggcga cgacatgaac
    19621 gacccggtgg gcgacaccgc ccgctcgatc ctcgacggcc acatcacact ctcgcgggcg
    19681 ctcgcctcgc aggggcactt ccccacgatc gacgtgctcg actcggtctc tcgtgtctcc
    19741 ggcgcggtca ccacgcccga gcagcagcga atggcccgcg agctgcgggc gctcctcgcc
    19801 gctcacaagg aagcgcggga tctgatcgag atcggcgcct accggcccgg gacgaacccg
    19861 acggtcgacc gtgccgtcgc cctccgcgac cggatcgagg cattcctccg ccaggggatg
    19921 aacgagacgt cggcgccggg tgtggcgtgg aaccagctcg ccgaactgct ggcgaccgac
    19981 accttcacgg ccgagatggc cggagcacag acaggcggtg gtgtcctgtg aagaagtaca
    20041 agttcgcctt cggaagcgtc caacgcgtgc agcggatcgc cgaagagcag gccgccgtgt
    20101 cgctcgccga ggcgcagcgg gcagccgacg cagccagcgc ccagctgcag gcgcgcctgg
    20161 ccgacatcgg agccgcgcgc cccgcgcccg gtcctcggag cacgaccgag ttccatgccc
    20221 agcgtgagca cctcgaccgt catcgcgtcg cggtcactgc ggcgagggcc gccgagatca
    20281 acgcgctcga gctcctcggc agcgctcggg ccgactgggt cgacgccgcc cgaaaggtcc
    20341 gcgcactcga ccgtctcgac gaacgcaagc gggccgagtg gacgctggag acgacccacg
    20401 ccgcacagct cgtcaccgac gagatcgcga ccatcaggta caagaccgaa caggaccgca
    20461 cctgatgtcc tccatcgcga cgttggccgg cgcgcattcc cgcatcgccg agatccagag
    20521 ccggttcgct ccgagctcgg tgcgagcgac cacggccacg cgaacgatcg acaccgcgtc
    20581 gttcgacgac gtgatgtcga acctgctcgg gtcgaccgct accgacaccg agggatcgac
    20641 gatcgatctg acgtcgctga cctcggtcct ctcgtcgctc ccgacccgcc gcagcggagc
    20701 cctcgccagt gccctcggca ccaccaccgg aacgtcgggc aacgggccca ccggcgccga
    20761 cgctgtcgca gccgccaagc agtacctcgg cgtggactac gtctggggcg gaacggaccc
    20821 ctccaagggg ctcgactgct ccggcctcgt gcaacgcgcc taccgcgatc tcggcatcga
    20881 gctcccccgc gtcagctacg accaggccaa ggtcggaacg aaggtcgact cgatcgaaca
    20941 ggcccggccc ggcgacctcg tggcgttcgg gagcccggtc gatcacatcg gcatctacgt
    21001 gggcgacggc aagatgatcc aggcgcccca caccggcgac gtggtccgga tctccgacat
    21061 caagcgcccg atcaccgcca tccgtcgcgt catccccgac gacgcggcgt cggcctcggg
    21121 catcaacgcc acctcgctcg ccgcagtaac cggtcgaacg accacaaccg gcggatcgac
    21181 cggcatcgcc ggcgccgaac cctacgagcg gctcttccag gcggcaggcg cgaagtacgg
    21241 cctgaacccg cgcctcctcg ccgccgtcgc caaggcggag tccaacttcg acccctccgc
    21301 ccgcagcggc gccggcgcgg tcggcctcat gcagttcatg ccggcgaccg ccgcgggcat
    21361 gggcatcgac cccaccgatc ccaaacaggc ggtcgacggc gcagcgaagt acctgcgcac
    21421 ccagctcgat cggttcggct cggtcgacct ggcgctcgcc gcctacaacg ccggtcccgg
    21481 tgccgtgcaa cgtgccggcg gcatcccgcc gtacgcagag acgcgcaact acgtgaccaa
    21541 gatcctcggg ctgctgggca accagtcccc cacgacctcg ccgaccctcg tcggctcgac
    21601 gaacggagtg ctgacgtgag caccgacctc ctgcccgtgc aatcgcggcc cgccgcccct
    21661 gccggttcca ccgccgacca gcgcgccggt gccggtgacg cagcccgggc cgcatcggag
    21721 ttcgaccgcg ccgtcgatca tgctgccgac aagctggccg gccgacaggg tggcaagcac
    21781 tcgacctcac accaggtgac ccggcacgat cgaccgaacc ggttgcgcga cggacgtgag
    21841 ggccaggacg gcgattccgg cgagaccggt gaggccaccg ctgcgggcac gctcccgacg
    21901 accgaagccg ccgagcccac gacggcgacc gacacggccg ctgccgcgac caccgatccg
    21961 acgtcgccgg cgccggtcgt ggtctcggtg ccgaccggcg ccgccgcggt cgcggccctg
    22021 ctcgccgcct ccgacgctgc gctcgccacg cccgatctca gcgtcaccac tcccacgacc
    22081 gacaccggcg atgccgtcgc cacggtcgac gggggcagcg acacgcctgc cgcgacctct
    22141 gccggcgaca ccgagccgac ccccaccgag accaacggca cgaccgctgt gagcgcagcc
    22201 ccggcagccg ccgacaccga cacgaccact gctgtcgctg tcaccggcac tgcaacgggc
    22261 tcc
//
LOCUS       DAOSOA010000016        18447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3028_length_18447_cov_5.560950, whole genome shotgun sequence.
ACCESSION   DAOSOA010000016 DAOSOA010000000
VERSION     DAOSOA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 18447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18447
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3028_length_18447_cov_5.560950"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..698)
                     /locus_tag="PKY13_02375"
     CDS             complement(195..698)
                     /locus_tag="PKY13_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT78799.1"
                     /translation="MTPIVLRDGTTLDVRAIGPDDAASLVEFHERLSIESTRLRFFSP
                     HPTLTAEEVERFTHVDHDRREAVLVLDGPEVVAVARYEGLPDDVAAEVAFVVAEAWQG
                     KGIATELLHRLAELAAGRGYQKLVADTLWENRAMVSVFEQSGLPEHHTIEQGVLHFAM
                     ELDAPTD"
     gene            complement(695..1546)
                     /locus_tag="PKY13_02380"
     CDS             complement(695..1546)
                     /locus_tag="PKY13_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78800.1"
                     /translation="MPLIDPRPALGGVVSALRAGFGRDLEAATLSAAEDAGLVTDRLG
                     EVVDAGDLEHLPGVAQRYLRFMGVEGAPRDTVITVHSRGRFRMRRHLPWFPYEAWQIN
                     TADPIARIYRIRIDAGPVPLIAVDTLLRGRGAMRGSVFGLVPVVDSSGPELDVGELVT
                     WLNDAVLLAPSMLLRPGVEWAAVDDRSFGVTVTDHGVAATARVVVDADGRPQAFETFD
                     RYVDLGHGLERARWTTPVRGWTTDGGHRRYSSGAAVWELPDGDFAYVRTRLSPGAVVH
                     GPSIRRP"
     gene            complement(1533..2432)
                     /locus_tag="PKY13_02385"
     CDS             complement(1533..2432)
                     /locus_tag="PKY13_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT78801.1"
                     /translation="MATITVGMDESDCAAEALRWAVAEGEARGWPVRAVMAWTFLRQH
                     HVDRTQTHDPEYSETDAMLALQEYVTDAVGADAAASIVMEVEAERAAVVLVEAAADSS
                     LLVVGSHGYGGFAGLFMGSVSQECLHHTTVPLAIIRGAEKRSARDGDPIEVKAGKVVV
                     GVDGSDTSRRALDWALDEARARGGWVEVVHSWMLPASYGYPTLVVPDLSIYEEAAEAV
                     VAELLDHADTSGMSRPISKVIVGGTSTSGLLAARSEDAELLVVGSRGIGGFAGMVVGS
                     VAHHLAHHATCPLVVLPAADAAH"
     gene            complement(2452..3798)
                     /locus_tag="PKY13_02390"
     CDS             complement(2452..3798)
                     /locus_tag="PKY13_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78802.1"
                     /translation="MTDETSDTTDTTHSTDLTRDRSLQGYWPSPWPAEDAGPTRPGCN
                     PDVGGPSFGSAPPAVVFREAIASTMVVLRDPGEVYVLCHSVGPDAVGWVERIDPITLE
                     PDARSADLPAGPVWPGGLAAHANGSLYVVFGRYAHRLDADLAVLASCRLPRDRPYNSF
                     VVLADGHLVTKDFGGVLPGEDPDRVAQPCELVVLEPERLDIVETVELAEPSIARLSSD
                     GDTVVVVGVSRLVRAAWDGERLTPDEAFDGRYLQVDGQTYGWDPVIALGAAWFLDNGF
                     GSERYAGTFRGQGVNTAPLHLVRVDLDTGAATLTEVCGLPDGVIANPPLIDVGRRIAV
                     AYDSGNGVLAAFDIAEDGSTALRWRHRQNHACHPLLFADTGELLTNDHDAERMADQLV
                     VRDIATGDEVARVDSGSIVQSVLFPTVGWDRDVYTCSFAGITRMSVVGSLDSVVAG"
     gene            4118..4426
                     /locus_tag="PKY13_02395"
     CDS             4118..4426
                     /locus_tag="PKY13_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78803.1"
                     /translation="MGTSWPTSRRAAIVAALTGILGYGTIQYRRNNRRAAIAALQDAV
                     EPPQFDDPLDATEIDLPTDVAHAPGHRHLPPPPTVNIPTSAPRARPRADKVGHPGRFS
                     "
     gene            4554..5093
                     /locus_tag="PKY13_02400"
     CDS             4554..5093
                     /locus_tag="PKY13_02400"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOT78804.1"
                     /translation="MADPVHNDLDALVPEDAGSLAVDRRDLDEQITAVSAGTVDLPEI
                     GPWISAAFGRVAATLGAQGVTPVGPPFARYFPVGNHRFHVEAGFPTDRAVEPDDDGVA
                     PSTLPAGPVAVTVHVGPYDGVAEAYERIATWAEEHGTTTSTDPWEFYLIDPTEQPDPS
                     TWRTEVFVPLDAEPARSSA"
     gene            5090..5650
                     /locus_tag="PKY13_02405"
     CDS             5090..5650
                     /locus_tag="PKY13_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78805.1"
                     /translation="MSRTTTDPTVPTVPTVPTDPAARRPDEIGTIADAVIRAHQELTD
                     AAARLRRLGTATEGDARLLQKAADANEAVVELEAEIIAAASRRLRIASSPRELGETLD
                     EVAASWRARAQDLVVQARLGKMEVDDAVTGALKDFEEAGDHLSELISTLRTHDTAWSD
                     ELRDHARVVVDDVRSAVSALLRAVER"
     gene            5647..6144
                     /locus_tag="PKY13_02410"
     CDS             5647..6144
                     /locus_tag="PKY13_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78806.1"
                     /translation="MNAFIDLTTASLDDSLAEVLAALDAACAAVDAGASPTSGRQSGD
                     EDLTELLVTIRLEMTVARARLSVDAATDLQGLVAAIELVATSAEVWLDDEVIRARLGA
                     MRPRDRATSVTCRIAGTATEMRRVAAEISDTDDLAGARSRARRAVQLARVALEHAALS
                     VRHAG"
     gene            6226..7572
                     /locus_tag="PKY13_02415"
     CDS             6226..7572
                     /locus_tag="PKY13_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT78807.1"
                     /translation="MRFTDRIEAGRLLARHLDHLGEDRPVVLGLPRGGVVVAAEVAGH
                     LGAPLDVLLVRKLGVPYQPEVAMGAIAEGGVEVVDRHVVDGLRITPDEVASVLAREEA
                     ELQRRLGLYRIGRTRIPLEGRTVVIVDDGVATGATARAACAAVREAGAARVVLAVPVA
                     PPGWDTAMDDTADEYVAVVTPDPFHSVGQFYARFTQTTDEEVADCLERSDRDPADEPL
                     VAGGAVDVIGGRDAEITIPIGPVRLGGHLTIPARPHGLVVFAHGSGSSHHSPRNRMVA
                     SILNDAGFGTLLFDLLTAEEERQRENVFDVELLADRLVAVTRWIRRDPDVAELPIGYF
                     GASTGAAAAMVAAAALGDGISAIVSRGGRLDLAGELISEVTAPTLLVVGARDTPVVDL
                     HHRLLPQLRCAHLLLLIDDATHLFEEPGALQRAAEAASDWFGEHLTATPHVPAEVS"
     gene            7572..8828
                     /locus_tag="PKY13_02420"
     CDS             7572..8828
                     /locus_tag="PKY13_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78808.1"
                     /translation="MGDLRLSPTPRRRRSPRRSPRRSARRLAATSALALALTSACVPP
                     AADLSLPTGTGLQWVHDTGGTTDENGTYHEAYARFTLLDGVTNGPTPVETPYLPLTPT
                     AGGAYASYGFPVFPDGTARFPWNGFAGEGVTTATTVTGGTTTYVVTGAGGSCTFANLS
                     TTGPMPRTSAAIPSPDGGKLAVFTRFADPDANSIRTTITIYALDASCTRLNGVTTTWN
                     SFAGSVSGAMIVSSLTVWSPDSASILYPVNLATARVGTRLERLAASGGSTPTTVLEDP
                     SSTILPLGWSSAGRALIFRISRSTGTPPVAATSLETLPIGGGHTKVIDVHTSLVPPTR
                     SEFHVGYFLPGTSQVVYSGGPRTATAGDGRTVAWPQLRIHDDTTDADVVVPGTDAPLA
                     WHPTPSGDMPNGRFLERFIHGTGATT"
     gene            8825..9265
                     /locus_tag="PKY13_02425"
     CDS             8825..9265
                     /locus_tag="PKY13_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78809.1"
                     /translation="MSDPHTTGPIRPKRRTTFDATPRRWALLAVTVLALVACTPAVGF
                     TPPFQGEARYQPSSGNFEGHMVACNADGGPITVTLGEWGFDLVHPFRVVVTDAAGGAV
                     ATLTVDGSDDVLVTQPLPRGTCFAVAITSPGTTGRSYYTYTVNW"
     gene            9323..9607
                     /locus_tag="PKY13_02430"
     CDS             9323..9607
                     /locus_tag="PKY13_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78810.1"
                     /translation="MTDTKTAWTQVGDHLSALCLKLKLHAEEELSDDEVCERAGLEKL
                     RSVIDETVEAITDAYADEAVREDARQLGRSFVAAVDATADELRTRVTTKR"
     gene            complement(9615..9857)
                     /locus_tag="PKY13_02435"
     CDS             complement(9615..9857)
                     /locus_tag="PKY13_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78811.1"
                     /translation="MTDQSSSSDPVTTDPESQDVAEMFDEEALGGDEVAPHHQELLAP
                     DLGGSDETAELVASFDDAEGPIGPEDAALHIEDERS"
     gene            9995..12394
                     /gene="ppsA"
                     /locus_tag="PKY13_02440"
     CDS             9995..12394
                     /gene="ppsA"
                     /locus_tag="PKY13_02440"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOT78812.1"
                     /translation="MAPPTPSSAPPVSSETGAPPICRFATLRSDDVARAGGKGANLGE
                     LTAAGLPVPDGFVVTADAYRSAMERAGVRDELRRAAAAVFAGEVADLAATVERLRATV
                     RSAGIPADLAALVDSAYRDLAGDEGAVVVRSSATSEDTAAASFAGMHDSFANVIGTEA
                     VRQRVLDCWVSLFSERALSYRADRGMGEEPTIAVVVQRLVPAVAAGVMLTDDPADPGV
                     GKLVIEAARGLGEAVVSGLVEPDTYVVDTGTHRVATTRRGAQHVALERRAGGGVEQID
                     LPDRDAGPVLADHQVLELARLGEQIAAHYGCPQDVEWVIAPTAVGPDGTDDADTMFIV
                     QSRPITTPGTTAAPTNGDATGPVAADGAVLVSGLGASAGTARGRVRILHSPSDGGLLL
                     DGEVLVAPTTSPDWVPVMRRAAAVVTDSGGRTCHAAIVTRELGVPCVVGTRSASSVLH
                     DGDLVTVDGRLGTVRAATTAIAAPERSTALVAVRADAAEPITLAREEPLATRVYVNLA
                     IASQAEAAAQLPVDGVGLLRAELMATDALHGEHPMAMLARGGREEFVSAMSNALLTVT
                     RAFGDRPVVYRSLDFRTNEFRGLVGGAAYEPVEANPMIGWRGCYRYVTEPDLFGLELE
                     TLARVREETPNLHLMIPFVRTLWELEQCLELVDASPLGRQRGLRRWVMAEVPSVAYRI
                     PEYAALGIDGVSIGSNDLTQLVLGVDRDSETCVELFDEGDAAVLDTIERIITAAHASG
                     ITASLCGQAPSNRPEFAEHLVRYGIDSISVTPDAVAPARRAIAVAERRLVVEAARRTL
                     G"
     gene            complement(12469..12915)
                     /locus_tag="PKY13_02445"
     CDS             complement(12469..12915)
                     /locus_tag="PKY13_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT78813.1"
                     /translation="MKITDALRKTGLSIGPGCTLRQAAELMECNAVGALAVVEDDRAV
                     GVVTDRDLVRRAVARGIAPDARVDSVMSSPVVSIADDADLHDAVAVFGRKAVRRLVVE
                     RDGRFVGVLSADDLLIDLASDLAAVVRPIGAEVLFGHRDSSVPATT"
     gene            complement(12920..13426)
                     /locus_tag="PKY13_02450"
     CDS             complement(12920..13426)
                     /locus_tag="PKY13_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78814.1"
                     /translation="MTLARGGAALAVTVHGFAHLAVYPLSGSVDPDVDPSRFWVPLSL
                     ARDASGWAARILLVVAVVGFLATGHQLAAGRRPTGWTVPAAGASLCSIAAVVVLWDDL
                     HPRPEALWVGGVVSAVVLVAVVATLVGGHRAVEPGHTVEGPGVMRGTFDPAAPAEPGA
                     PLGDTPRR"
     gene            13610..15103
                     /locus_tag="PKY13_02455"
     CDS             13610..15103
                     /locus_tag="PKY13_02455"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT78815.1"
                     /translation="MDVPTTATGPGPAPGPTPELLPAGIAETHSAVVVFLGDRAYKIK
                     KPVDLGFLDFSTPEARAEACENEVELNRRLSPDVYLGVAEIGDTEGGVCDHMVVMRRL
                     PEDRKLRACLDRGEDVTDAIRAVARDVAVLHGRATTGPSHTYCAEPAAVRRNWTDGFA
                     QMAPFVGELLDPDVQARIEQLALRFLDGRGPLLERRISRGHVRDGHGDLQAEDIFLLD
                     DGPRILDCLDFDAGLRWGDVLLDVGFLAMDLERLGHPDVAARFLADYREFSAENWSAS
                     LADHYIAYRAHVRAKVATLRAAQTGERTDDIAQLQRLCLEHLERARIRLVVVGGLPGT
                     GKSTVAAALGNRAGAVVLRTDEIRNQMTSGALVSTADRYSPVGVDAVYQETLCRAERL
                     LRRGEQVVIDATWSSSAHRDEVRAVAERNGADLVEIECHAPQAVCDQRIRERLDLGTD
                     PSEATPEVAAAMAARFAPWPEATAVSTTTPLELTIDQAALAAGWESL"
     gene            15100..15927
                     /locus_tag="PKY13_02460"
     CDS             15100..15927
                     /locus_tag="PKY13_02460"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HOT78816.1"
                     /translation="MNDLTDPTDPTDANDPEDRARRSRWVRMIAPAVAVAAVAVAALV
                     IGVLADDDEDVSTASTTSAAPTTTTTTTSVAITTTTSTPASTTTVDTSATDAAVWPAP
                     SSGRRFTDPVEAATTFATEFVGFTDPVVGEFLQGDSRSGEVEVRPRADGPVTTVFVRL
                     LGTDDAWSVLGTATPNIEVDQPTALTTIGNPVRLIGRARAFEGTVDVEVREDGTTTPL
                     GTGFVTGRGDGVLGPFDSELPYEVAGTERGTVLFVTHSAEDGSVWEAAVIRVVFPTP"
     gene            15944..17350
                     /locus_tag="PKY13_02465"
     CDS             15944..17350
                     /locus_tag="PKY13_02465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOT78817.1"
                     /translation="MDRLSALDTEFLHLEDDTSPMHIASVCVFAGPAPSLTELTDLLE
                     SKLHELPRYRQVIRTVPLELGRPIWVDDRRFRLDYHVHRTALPSPGGAAELDDLMGRL
                     MAHPLNRSRPLWEAWLVEGLSDDRWALVFKVHHCMVDGVAGVHLLEAFLDVSPDTPPG
                     TVRPWRAQPEPSPLGKVVGSWVGLAGDVIPWGRRIATAASSPIDAVRDVAAISTGLGS
                     FLAHLVPAPGNELQGRIGPHRRWSHVTLDLSDVALVRRRLGGTVNDVLLACVTAGHRA
                     LLEAHGDDPSTAVVRTAIPVSTRDWGTSGSGNRVSTLLAELPVGQPDPLVRLATVREF
                     TTRLKASSMASVADTFFTLADLAPPLVVGPISRLAIRLEHRRAQRSITTITTNVPGPP
                     FPLYCLGREMIEQLPYVGLTDGVRVTTAIVSYNGHITVGVTGDEDSVPDVGVATAAIG
                     DALAELVRLARRRKDRTR"
     gene            17347..18372
                     /locus_tag="PKY13_02470"
     CDS             17347..18372
                     /locus_tag="PKY13_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOT78818.1"
                     /translation="MTDTMRAWVVDRPGPVATGAPLVEVERPLPVPAAGQVRVRVSCC
                     GVCRTDLHLAEGDLSPHARRVVPGHEIVGVVDALGSDSRRFALGERIGIAWLATTCRT
                     CRFCRSARENLCTAPTFTGWDHDGGYAEFALVDEGYAYRLPDGFDDLTAAPLLCAGII
                     GYRALARADLPPGGRLGIYGFGASAHLAAQVAIAGGAEVHVMTRSPAARALAMRLGAA
                     SAQGAADPPPVPLDASILFAPVGTLVPAALEGLDRGGTLSIAGIHLSDIPSLDYQRHL
                     FEERTLRSVTANTREDGERFLAVAADIGISVETTEYPFDEADTALADLAADRVDGAAV
                     LRMPSAR"
BASE COUNT         2743 a         6922 c         6248 g         2534 t
ORIGIN      
        1 cccaggtgct caacgccgtg ctgctgctcc cgctcctcgt gttcatggtg tcggcgggcc
       61 gcgacgtcga cctgatgggc gacctcgcca ccggccgtcg tggtgccatc gcccagggtg
      121 ccgtgatcgc cgtggtcacc gtgtgcgtcg gtgcgctcgg ggtgctcggg gtgacctgag
      181 gcggggcgcg gcggtcagtc ggtcggcgcg tcgagctcca tggcgaagtg caggacccct
      241 tgttcgatcg tgtggtgctc gggaaggccc gactgctcga acaccgacac catcgcccgg
      301 ttctcccaca aggtgtcggc cacgagcttc tggtagccgc gcccagccgc cagctcggcg
      361 agccggtgca acagctcggt ggcgatgccc ttcccctgcc acgcctcggc cacgacgaac
      421 gccacctcgg ccgcgacgtc gtcgggcaac ccctcgtacc gggcaaccgc cacgacctcg
      481 gggccgtcca acaccagcac cgcctcgcga cggtcgtggt cgacatgggt gaaccgctcg
      541 acctcctccg ccgtcagcgt gggatgcgga ctgaagaacc ggaggcgagt ggactcgatc
      601 gacaggcgct cgtggaactc gacgagggac gcggcatcgt cgggcccgat cgcccggacg
      661 tcgagcgtcg tgccgtcacg gaggacgatc ggggtcacgg ccgccggatc gacggaccgt
      721 gcacgaccgc gccgggagac aaccgggtcc ggacgtaggc gaagtcgccg tcgggcagct
      781 cccacaccgc agcgccgctc gaataccggc gatgtccgcc gtcggtcgtc cacccacgga
      841 ccggcgtcgt ccagcgggcg cgctccaggc cgtgacccag gtcgacgtag cggtcgaagg
      901 tctcgaaggc ctgcggtcgt ccgtcggcgt cgaccacgac acgggccgtc gccgcgacgc
      961 cgtggtcggt gacggtgacc ccgaagctcc ggtcgtcgac cgccgcccat tcgaccccgg
     1021 gtcgcaggag catcgacggc gccaggagca cggcatcgtt caaccacgtc acgagctcgc
     1081 ccacgtcgag ctccggtccg gaactgtcca ccaccggcac caacccgaac acgctgccgc
     1141 gcatggcgcc tcgacctcgc agcagggtgt cgacagcgat cagcgggacc ggcccggcgt
     1201 cgatgcggat gcggtagatg cgggcgatcg ggtcggcggt gttgatctgc caggcctcgt
     1261 agggaaacca gggcaggtga cgccgcatcc ggaaccggcc gcgggagtga accgtgatga
     1321 ccgtgtcgcg aggtgcgccc tcgacgccca tgaaccggag gtaccgctgg gcgacgccgg
     1381 gaaggtgctc gaggtctccg gcgtcgacga cctcgccgag ccgatcggtg accagtcctg
     1441 cgtcctcggc ggccgacagc gtcgccgcct cgagatcgcg cccgaacccg gcacggagcg
     1501 ccgagacgac gccgccgagc gctgggcgag gatcaatgag cggcatcggc ggcaggcagg
     1561 acgacgagcg ggcaggtcgc gtggtgggcc aggtggtggg cgaccgatcc gaccaccatg
     1621 ccggcgaagc caccgatgcc ccgggagccg acgacgagga gctcggcgtc ctcggagcgg
     1681 gcggcgagca gccccgacgt cgacgtgccg ccgacgatga ccttcgagat cggccgtgac
     1741 atgcccgaag tgtcggcgtg gtcgaggagt tcggcgacga ccgcctcggc ggcctcctcg
     1801 tagatcgaca ggtcggggac gaccagggtc gggtagccgt aggatgccgg gagcatccag
     1861 ctgtggacga cctcgaccca tccgccgcga gcgcgggcct cgtcgagcgc ccagtcgagg
     1921 gcacggcgcg acgtgtcgga accgtcgaca ccgacgacga ccttgcccgc cttcacctcg
     1981 atggggtcgc cgtcgcgggc gctgcgcttc tcggcacccc ggatgatggc gagcggcacc
     2041 gttgtgtggt gcagacactc ctggctcacc gaccccatga agagcccggc gaaaccgcca
     2101 tagccgtgcg agccgacgac caggagcgac gagtcggcgg ccgcctcgac gagcaccacg
     2161 gccgcacgct cggcctcgac ctccatcacg atcgaggccg cagcgtcggc accgacggcg
     2221 tcggtcacgt actcctgcaa cgcgagcatt gcgtcggtct cggagtactc cgggtcgtgc
     2281 gtctgggtcc ggtcgacgtg gtgctgccgg aggaacgtcc acgccatgac ggctcggacg
     2341 ggccagcctc tcgcttcacc ttctgcgacc gcccagcgca gcgcctccgc ggcgcagtcc
     2401 gattcgtcca ttcccacggt gatcgttgcc atgactgcat cgtcatcgag atcaccccgc
     2461 cacgacagag tccaaggacc cgaccacgct catccgcgtg atgccggcga acgagcaggt
     2521 gtagacgtcg cggtcccacc ccacggtggg gaagagcacc gactgcacga tgctgcccga
     2581 atcgactcgg gccacctcgt cgcccgtcgc gatgtcgcgc accacgagct ggtcggccat
     2641 ccgctcggcg tcgtgatcgt tggtgagcag ctccccggta tcggcgaaga gcaacgggtg
     2701 gcaggcatgg ttctgccgat gccgccaccg caacgccgtc gaaccgtcct cggcgatgtc
     2761 gaacgccgcg agcacgccgt tgccactgtc gtaggcgacc gcgatgcgac ggccgacgtc
     2821 gatgagcggc gggttggcga tgaccccgtc ggggagccca cacacctcgg tcaaggtcgc
     2881 cgccccggtg tcgaggtcga cccggacgag atggagcggg gccgtattga cgccctgacc
     2941 acggaaggtg ccggcatacc gctcgctgcc gaacccgttg tcgaggaacc acgccgcgcc
     3001 gagcgcgatg acgggatccc agccatatgt ctggccgtcg acctgcaggt agcgcccgtc
     3061 gaacgcctcg tcgggggtga gccgctcgcc gtcccacgcc gcccgcacga gccgtgaaac
     3121 accgaccacg acgaccgtgt cgccgtcgga ggagagtcgg gcgatcgacg gctcggccaa
     3181 ctccacggtc tccacgatgt cgagtcgctc cggctcgagc acgacgagct cgcacggttg
     3241 ggcgactcga tcggggtcct cccccgggag caccccgccg aagtccttcg tcacgaggtg
     3301 accgtcggcg aggacgacga agctgttgta gggccgatcg cgcgggagtc ggcacgacgc
     3361 cagcaccgcg agatcggcgt cgagccggtg ggcgtaccga ccgaacacga cgtagagcga
     3421 gccgttggcg tgcgccgcga gaccgcccgg ccacaccggc ccggccggga ggtcggcgga
     3481 acgggcgtcc ggttcgagcg tgatcggatc gatgcgctca acccagccga ccgcgtcggg
     3541 gccgaccgag tggcagagca cgtagacctc gccggggtcg cggagcacga ccatcgtgga
     3601 cgcgatcgcc tcccggaaga cgacggcggg cggcgccgac ccgaaggacg gcccgccgac
     3661 atcggggttg cagccggggc gcgtcggccc ggcgtcctcg gcgggccagg gcgatggcca
     3721 gtagccctgc agcgatcggt cgcgggtgag gtcggtcgag tgggtcgtgt cggtcgtgtc
     3781 gctggtctcg tcggtcacgg cgccatcctg ccccgtcgag cgcccggtcg ggatgaccac
     3841 gctgttccgg acgggctcga cggcgacggc ggtgaggccg ccaaaacgaa gacggacacg
     3901 tgcaaatcca tgcagattgt gccgctccgc gtcactatct gcctggattt cggctcaggc
     3961 tcgacgccct cgggcctcag ccgccggcat ggggctcgcc ggcgcaccac cgggcgcgtg
     4021 accttcggcc ctgccgcacg gacccgccga ggcgaagggt ggactcgacg gggttcgtca
     4081 gcggacgccg tcgacccgac acacaaggag caccctcatg ggcaccagct ggccaacctc
     4141 tcggcgggcg gcgatcgtcg ccgcgctcac cggcatcctc ggttacggaa cgatccagta
     4201 tcggcgcaac aaccgccggg cggcgatcgc cgcgctccag gacgccgtcg agccgccgca
     4261 gttcgacgat ccgctcgacg cgaccgagat cgacctgccc accgacgtcg cacacgcgcc
     4321 cgggcatcgg catctcccgc cgcccccgac ggtgaacatc ccgacctcgg ctccccgggc
     4381 gcgaccccga gccgacaagg tcgggcatcc cggtcgcttc agctgaccca cgacgggacc
     4441 tacgtccctg gccggacggc ggcccgggcc tgatcatcga gatggggcac acgcggagct
     4501 gcccgtccgg acaccaaccg cgggacccga cccacccgat gaaggagcag gccatggccg
     4561 acccggtgca caacgatctc gacgcactcg ttcccgagga cgcgggctcg ctggccgtcg
     4621 accgacgcga cctcgacgag cagatcacgg cagtgagcgc cgggaccgtc gacctgcccg
     4681 agatcggacc gtggatctcc gcggcgttcg gtcgtgtcgc cgccacactc ggcgcccagg
     4741 gcgtcacgcc cgtcggtccg ccgttcgccc ggtacttccc tgtcggcaac caccggttcc
     4801 acgtcgaggc aggattcccg accgaccggg ccgtcgagcc cgacgacgac ggcgtcgccc
     4861 cgtcaacgct gccggcgggg ccggtcgccg tgaccgtgca cgtcggcccc tacgacgggg
     4921 tggccgaggc ctatgaacga atcgccacct gggccgagga acacggcacg accaccagca
     4981 ccgatccgtg ggagttctac ctcatcgatc cgaccgaaca acccgacccc tccacctggc
     5041 gcaccgaggt cttcgtaccc ctggacgccg aaccggcaag gagctcagca tgagccgcac
     5101 gaccaccgat cccaccgtcc cgacggtccc gacggtcccg accgacccgg ccgcacgtcg
     5161 gcccgacgag atcggaacga tcgccgacgc cgtcattcgg gcacaccagg agttgaccga
     5221 cgccgcagca cggctccggc gcctgggcac cgcaacggag ggcgacgccc gcctcctcca
     5281 gaaggccgcg gacgcgaacg aggccgtcgt cgagctggag gccgagatca tcgccgccgc
     5341 ctcgcggcgt ctccgcatcg cgagctcgcc ccgagaactc ggcgagacgc tcgatgaggt
     5401 cgcggcctcc tggcgggccc gagcccagga cctggtcgtc caggcgcgcc tcggcaagat
     5461 ggaggtcgac gacgcggtca ccggcgcgct caaggacttc gaggaggccg gcgaccacct
     5521 ctccgagctg atctcgaccc tccggacaca cgacacggca tggtccgacg agctccgcga
     5581 ccacgcccga gtggtcgtcg acgatgttcg gtcggcggtc tcggcgctcc tcagagcggt
     5641 cgagagatga acgcgttcat cgatctgacg acggcgagcc tggacgattc gctcgccgag
     5701 gtcctggcag cgctcgacgc cgcgtgtgcc gcggtggacg ccggcgcctc ccccacgtcg
     5761 ggacggcagt cgggcgacga ggacctcacc gagctgctcg tcacgatccg gctggagatg
     5821 accgtggccc gagcgcgcct gtccgtcgac gccgccaccg acctccaggg cctcgtcgcc
     5881 gcgatcgaac tggtcgccac ctccgccgag gtgtggctcg acgacgaggt catccgtgcc
     5941 cgcctcgggg cgatgcgtcc tcgcgaccgg gccaccagcg tcacctgtcg aatcgccgga
     6001 acggcaaccg agatgcgccg ggtcgccgcc gagatcagcg acaccgacga tctcgcgggt
     6061 gcccgctctc gtgcccgccg cgccgtgcag ctggcgcggg tcgcgctcga gcacgcagcg
     6121 ctgtcggtcc gccacgccgg ctgagccggc gggaccatcg gcccttccat cacaccgcca
     6181 acgggacgat cctgcagtga gggtccgtcg aacggaggag tcaccatgag gttcaccgat
     6241 cgaatcgagg ccggccgatt gctggcgcga catctcgacc acctcggcga ggatcgaccc
     6301 gtcgtgctcg ggttgccccg cgggggtgtc gtcgtcgccg ccgaagtcgc cggacacctg
     6361 ggtgcacccc tcgacgtcct cctcgtgcgc aagctcggcg tcccctacca acccgaggtc
     6421 gccatgggcg ccatcgccga gggtggcgtc gaggtcgtcg atcgccatgt cgtcgacgga
     6481 ctccggatca caccggacga agtggcttcg gtcctggccc gcgaagaggc cgagctgcag
     6541 cgccgactgg gcctctaccg gatcggccga acccgtatcc cgctcgaagg acgcaccgtg
     6601 gtcatcgtgg acgacggggt ggcgaccggg gcgaccgccc gcgctgcgtg cgccgcagtt
     6661 cgggaagcgg gcgccgcacg cgtcgtgctc gccgtcccgg tggcaccgcc cggttgggat
     6721 acggcgatgg acgacaccgc cgacgagtac gtcgcggtcg tcacccccga tccgttccac
     6781 tcggtcggcc agttctacgc ccgcttcacc cagaccaccg acgaggaggt cgccgactgc
     6841 ctggagcggt ccgaccggga tccggccgac gagccgctcg tcgccggcgg cgctgtcgac
     6901 gtcatcgggg gccgcgacgc cgagatcacg atcccgatcg ggcccgtccg cctgggtggg
     6961 catctcacga tccccgcccg gccgcacggc ctcgtcgtgt tcgcccacgg cagtgggagc
     7021 agccaccaca gcccgaggaa ccggatggtc gcctcgatcc tgaacgacgc cggcttcggc
     7081 accctcctct tcgacctcct caccgctgag gaggagcggc agcgggagaa cgtgttcgac
     7141 gtcgaactgc tcgccgatcg cctcgtcgcc gtcacccggt ggatccgccg ggatcccgac
     7201 gtcgccgagc tcccgatcgg atacttcggg gcgagcaccg gggcggctgc agcgatggtg
     7261 gcagcagcgg ccctcggtga cgggatctcc gccatcgtgt cccgtggggg tcggctcgat
     7321 ctcgccggcg agctcatctc ggaggtcact gcaccgacac tcctcgtggt cggcgcccgg
     7381 gacactccgg tggtcgacct ccatcatcgt ctcctgccgc agctccgctg cgcccacctg
     7441 ctcctgctga tcgacgacgc gacccacctc ttcgaggaac cgggcgcact ccaacgagcc
     7501 gccgaggccg ccagcgactg gttcggggaa cacctgaccg cgacaccgca cgtcccggcg
     7561 gaggtctcgt gatgggcgac ctgcgtctct ccccaacccc tcgtcggcgg cggtcccccc
     7621 gccggtctcc ccgtcggtcg gctcgacggc tggcggcgac gtccgcgctc gcactcgcgc
     7681 tgacgtccgc gtgcgtccca ccggcggccg acctgtcgct gccgacgggg acggggctcc
     7741 aatgggtgca cgacaccggc ggtacgaccg acgagaacgg cacctaccac gaggcgtatg
     7801 cacggttcac cctgctcgac ggggtgacca acggtccgac accggtcgag acgccgtacc
     7861 tcccgttgac cccgactgcc ggcggcgcct acgcgtcgta cggcttcccg gtcttccccg
     7921 acgggaccgc tcgattcccc tggaacggtt tcgctggcga aggggtcacg accgccacca
     7981 ccgtgaccgg cggaacgacg acctacgtgg tcaccggcgc cggcggatcg tgcacgttcg
     8041 ccaacctgtc cacgacaggg ccgatgccac gaacctcggc cgccatcccg tcgcccgacg
     8101 gtggcaagct cgcggtgttc acgcggttcg ccgatccgga cgcgaactcg atccggacga
     8161 cgatcacgat ctacgcgctc gacgcctcgt gcacccgcct caacggcgtg accaccacgt
     8221 ggaactcgtt cgccggctcc gtcagcggcg ccatgatcgt cagctcgctc accgtgtggt
     8281 cgccggactc ggcgtcgatc ctctacccgg tcaacctcgc cacggcacgg gtcggcacgc
     8341 ggctcgagcg actggcggcg tcgggcggat cgacgcccac caccgtgctg gaggacccgt
     8401 cgtccacgat cctgccgctc ggctggtcgt cggccggtcg agcactgatc ttccggatca
     8461 gccgaagcac cggcaccccg ccggtggccg cgacgtcgct cgagacgctc ccgatcggcg
     8521 gcgggcacac caaggtcatc gacgtccaca cgagcctcgt cccgcccacc cgatccgagt
     8581 tccacgtcgg ctacttcctg cccggcacct cacaggtcgt ctacagcggg ggcccacgca
     8641 ccgcgacggc aggcgacgga cgcaccgtgg cgtggccaca actccggatc catgacgaca
     8701 cgaccgacgc cgatgtcgtc gtgcccggca ccgacgcccc actggcctgg catccgacgc
     8761 cgtcgggcga catgcccaac ggtcggttcc tcgaacgatt catccacgga acgggagcaa
     8821 cgacatgagc gatccacaca cgacgggccc gatccgaccg aagcgccgga ccaccttcga
     8881 cgcgaccccc cgacgatggg cactgctcgc tgtcaccgtg ctcgcgctcg tcgcctgcac
     8941 ccccgctgtg gggttcactc ctccgttcca gggcgaggcc cgctaccaac cgtcgtcggg
     9001 caacttcgag ggccacatgg tcgcctgcaa cgccgacggc gggccgatca ccgtcaccct
     9061 cggcgagtgg ggcttcgacc tcgtgcaccc gttccgtgtc gtcgtgaccg atgcggccgg
     9121 gggtgccgtg gcgacgctga ccgtcgacgg ctccgatgac gtgctcgtga cccagccact
     9181 cccacgcggc acctgcttcg cggtggcgat cacgtcgccc gggacgacag ggcgcagcta
     9241 ctacacctac accgtgaact ggtgaggttc ctcgggacca acggcccttc ccgctccgtc
     9301 acatcgagcg caggctcggg gcatgactga caccaagact gcatggaccc aggtcggcga
     9361 ccacctctcg gcgctgtgcc tcaagctcaa gctccacgcc gaggaggagc tgtccgacga
     9421 cgaggtctgc gaacgtgccg gcctcgagaa gctccgctcg gtgatcgacg agaccgtcga
     9481 ggccatcacc gacgcgtacg ccgacgaggc ggtgcgggag gacgcacgac agctgggacg
     9541 atccttcgtc gccgccgttg acgccacggc cgacgaactt cgcacccggg tcaccaccaa
     9601 gcgctgacga cggctcagct gcgctcgtcc tcgatgtgga gcgcggcgtc ctcggggccg
     9661 atcgggccct cggcgtcatc gaacgacgcg acgagttcgg cggtctcgtc cgagccgccg
     9721 aggtcggggg cgaggagctc ctggtggtgc ggggccacct cgtcgccgcc gagcgcctcc
     9781 tcgtcgaaca tctcggcgac gtcctggctc tcggggtcgg tggtcacggg atcgctggag
     9841 ctgctctggt cggtcatggt ggtcctttcg acggtcgaca ccagcgtgca cccgaccgtc
     9901 gtcgaacgac agggccgaag gtccggaccg ccacgtccgg gatccgggac ctaggcctct
     9961 acctcgatcc gtcggcgggg tgtcccctgg agagatggcg cctccgaccc cgagttccgc
    10021 tcccccggtc agttcggaga cgggggcgcc acccatctgc cggttcgcca cgctacgcag
    10081 cgacgacgtc gcccgcgccg gcggcaaggg tgcgaacctc ggtgagctga ccgcagcggg
    10141 cctccccgtg cccgacggct tcgtcgtcac cgccgacgcc taccgctccg ccatggagcg
    10201 ggcgggggtg cgcgacgagc tccgtcgggc ggccgcagca gtgttcgccg gcgaggtcgc
    10261 cgacctggcc gccacggtcg aacgattgcg cgccacggtc cgatcggccg ggatccccgc
    10321 cgatctcgcc gcgctcgtcg acagcgccta ccgcgatctg gccggagacg agggcgccgt
    10381 cgtcgtgcgg tcgtcagcga ccagcgagga caccgcggcg gcgtcgttcg ccgggatgca
    10441 cgactcgttc gccaacgtca tcgggaccga agccgtccgg cagcgtgtgc tcgactgctg
    10501 ggtgtcactg ttctccgagc gcgccctgtc ctaccgcgcc gaccgcggca tgggcgagga
    10561 gcccaccatc gcggtcgtgg tgcagcgtct cgtcccggcc gtcgccgccg gcgtcatgct
    10621 gaccgacgac ccggccgacc cgggggtcgg caagctcgtc atcgaagcgg cccggggcct
    10681 cggcgaagcg gtcgtgagcg ggctcgtcga accggatacc tacgtggtcg acacgggtac
    10741 ccaccgtgtc gccacgaccc gtcgcggcgc ccaacacgtc gcgctcgaac ggcgcgccgg
    10801 cggcggggtc gaacagatcg acctgccgga ccgcgacgcc ggcccggtcc tcgccgacca
    10861 tcaagtcctc gaactggcac ggttgggcga acagatcgcc gcccactacg gctgcccgca
    10921 ggacgtcgag tgggtcatcg caccgacggc ggtcgggccc gacggcaccg acgacgcgga
    10981 cacgatgttc atcgtgcagt cccggcccat cacgaccccc ggcacaacgg cagcgccgac
    11041 gaacggcgac gcgacaggcc cggtcgcggc cgatggtgcg gtcctcgtgt cggggctcgg
    11101 agcgtcggcg ggcacggctc gcggccgcgt ccgcatcctg cactcgccga gcgacggcgg
    11161 attgctgctc gacggcgagg tgctcgtcgc gccgacgacc agtcccgact gggtgccggt
    11221 gatgcgtcgc gccgccgccg tggtgaccga cagcggcggg cggacctgcc atgccgccat
    11281 cgtgacccgc gagttggggg tgccctgcgt ggtcgggacc cgctccgcat cgagcgtcct
    11341 gcacgacggc gacctcgtca ccgtcgacgg tcgtctcggg accgttcggg ccgccacgac
    11401 ggccatcgcc gctcccgaac gctcaaccgc gctcgtggcc gtccgcgccg atgccgcgga
    11461 gcccatcacc ctcgcccggg aggaaccgct cgccacacgg gtctacgtga acctggcgat
    11521 cgcttcccag gccgaagccg ccgcacaact ccccgtcgac ggcgttgggc tgctccgggc
    11581 cgagctcatg gccaccgacg ccctgcacgg tgagcacccc atggcgatgc tcgcccgtgg
    11641 tggtcgcgag gagttcgtct ccgcaatgtc gaacgccctc ctcaccgtca cacgggcctt
    11701 cggcgaccgt ccggtcgtct accggagcct cgacttccgc accaacgagt tcaggggcct
    11761 ggtcggcggc gcggcctacg aaccggtcga agcgaacccg atgatcggat ggcgcggctg
    11821 ctaccgctac gtgaccgaac ccgacctgtt cggcctcgag ctcgagacgc tcgctcgggt
    11881 gcgggaggag actccgaacc tgcacctgat gatcccgttc gtccgcacac tgtgggagct
    11941 cgagcagtgc ctcgaactgg tcgacgcctc cccgctcggg cgccaacgcg gcctgcgacg
    12001 ctgggtgatg gccgaagtgc cgtcggtcgc gtaccggatc cccgagtacg cggccctcgg
    12061 catcgacggc gtctcgatcg gatcgaacga cctgacccaa ctcgtactcg gcgtcgaccg
    12121 tgactccgag acgtgtgtcg aactcttcga cgagggcgac gccgcggtcc tcgacaccat
    12181 cgagcggatc atcaccgccg cccacgcctc gggcatcacc gcctcgctct gcgggcaggc
    12241 gccgtcgaac cgacccgagt tcgccgagca cctcgtccgc tacggcatcg actccatctc
    12301 ggtcaccccc gacgccgtgg ccccggcgcg ccgggcgatc gccgtcgcgg aacgacggct
    12361 ggtcgtcgaa gcggcccgac gcaccctcgg ctgacgatct cctgcgggcg ccggcgcgag
    12421 cggcgccgac gccggcaggg cagcgtgagc cccagcgtga gccccgcgtc aggtggttgc
    12481 ggggaccgag ctgtcgcggt gcccgaacag cacctcggca ccgatcgggc gaaccaccgc
    12541 cgccagatcc gacgccaggt cgatcagcag atcgtccgcc gacaagacac cgacgaatcg
    12601 gccgtcgcgc tcgacgacga gccgacggac cgccttccga ccgaacaccg cgactgcgtc
    12661 gtggaggtcg gcgtcatcgg cgatcgacac gaccggcgag ctcatgaccg agtcgacgcg
    12721 ggcgtcgggc gcgatgccac gggccaccgc ccgacggacc aggtcgcggt cggtgacgac
    12781 accgaccgcc cggtcgtcct cgacgacggc gagtgcaccc acggcattgc actccatcag
    12841 ctcagcggcc tgccgcaacg tgcaccccgg gccgatggag aggccggtct tgcgtagtgc
    12901 gtcggtgatc ttcatggtcc tacctcctgg gtgtgtcccc caagggtgcg cccggttcgg
    12961 cgggcgcggc agggtcgaag gtcccccgca tcacgccagg accttcgacc gtgtgaccgg
    13021 gctcgacggc gcggtgaccg ccgacgagcg tcgccacgac ggcgacgagg acgaccgcgc
    13081 tgacgacacc gccgacccac agcgcctcgg gtcgggggtg caggtcgtcc cacaacacca
    13141 caacggcggc gatcgaacac agcgacgctc ccgcagcggg caccgtccag ccggtcggcc
    13201 gccggccggc agcgagctga tggccggtgg cgaggaagcc cacgaccgcg accacgagca
    13261 ggatccgggc cgcccatccg gaggcgtctc gggcgagcga cagcggcacc cagaacctcg
    13321 acggatcgac gtcgggatcg accgaacccg acagcgggta gaccgcgagg tgagcgaagc
    13381 cgtgcaccgt cactgcgagc gctgcaccac ctcgcgccag ggtcacgcgg ggccgtcggc
    13441 tcgtcgggcg ttcttggtcg ggcacgcagc gtctcccccg gtcggttcac acgatcgtcc
    13501 ctcgcagcgc cgctcggacc cagggtcgaa ggtccccggt gcgggcgctc gggcccatcg
    13561 gtgaccttgg accctgggtc cgccgaccac cggggcgcag actcggggca tggatgtccc
    13621 gaccaccgcg accggtcccg gcccagcccc cgggccaacc ccggagctcc tcccggccgg
    13681 gatcgccgag acccattccg ccgtggtggt cttcctcggc gatcgcgcct acaagatcaa
    13741 gaagcccgtc gatctgggct tcctcgactt ctccacgccc gaggcacggg ccgaggcctg
    13801 cgagaacgag gtcgagctca atcgtcggtt gtcccccgac gtgtacctcg gggtcgccga
    13861 gatcggcgac accgaaggtg gtgtgtgcga ccacatggtg gtgatgcgtc gcctcccgga
    13921 ggatcgcaag ctgcgagcct gcctcgaccg cggcgaggac gtgaccgacg ccatccgggc
    13981 cgttgcccgg gacgtcgcgg tcctgcacgg tcgggcgacg accgggccgt cgcacaccta
    14041 ctgcgcggag ccagccgcgg ttcgacgcaa ctggaccgac gggttcgccc agatggcccc
    14101 gttcgtcggc gagctgctcg atccggacgt gcaggcgcgg atcgagcagc tcgcgctgcg
    14161 cttcctcgac ggccgcggcc cgctgctcga gcgccggatc tcccgcggtc acgtccgcga
    14221 cggccacggc gacctccagg ccgaggacat cttcctgctc gacgacggac cgcggatcct
    14281 cgattgtctc gacttcgacg ccgggctccg ctggggcgac gtcctgttgg acgtcgggtt
    14341 ccttgccatg gacctcgaac ggctgggcca ccccgacgtc gcggcgcgct tcctcgccga
    14401 ctaccgcgag ttctccgccg agaactggtc ggcgtcgctc gccgaccact acatcgccta
    14461 ccgggcccac gtgcgagcca aggtcgcgac cctccgcgct gcccagaccg gtgagcgcac
    14521 cgacgacatc gcccagttgc agcgactgtg cctcgagcac ctcgaacgcg cccggatccg
    14581 gctcgtggtc gtcggtgggc tgccgggcac ggggaagtcg acggtcgccg cagcgctcgg
    14641 caaccgtgcc ggggccgttg tgctccgcac tgacgagatc cgcaaccaga tgaccagcgg
    14701 tgcgctcgtc tcgacggccg accgctactc ccccgtcggc gtcgacgccg tctatcagga
    14761 gaccctgtgc cgggccgaac gcctgctgcg ccgcggtgaa caggtggtca tcgatgccac
    14821 gtggtcgagc tccgcccatc gcgacgaggt cagggcggtc gccgaacgga acggtgccga
    14881 cctggtcgag atcgagtgcc atgcgccgca ggcggtgtgc gatcagcgaa tccgcgagcg
    14941 cctcgacctc gggaccgacc cctccgaggc gacccccgag gtcgctgccg ccatggcggc
    15001 gcggttcgcg ccgtggcccg aggcgaccgc cgtgtcgacg accacccctc tcgagctgac
    15061 gatcgaccag gcggcgctcg ccgcaggatg ggaatcgctg tgaacgacct gaccgatccg
    15121 accgatccga ccgatgcgaa cgaccccgag gaccgggcca ggcgctcgcg ctgggtccgg
    15181 atgatcgcac ccgcggtcgc cgtggcggcc gtcgccgtcg ccgcgctcgt catcggcgtg
    15241 ctggccgacg acgacgagga cgtcagcact gcgtccacga cgtccgcggc cccgaccacg
    15301 accaccacca ccacgtccgt ggcgatcacc acgaccacgt cgacaccggc gagcacgacg
    15361 accgtcgaca cctctgccac cgacgcagct gtctggccgg cgccgtcctc gggtcggcgc
    15421 ttcaccgacc cggtcgaggc ggcaacgacg ttcgcgaccg agttcgtcgg tttcaccgac
    15481 cccgtcgtcg gcgagttcct ccagggcgac agccggtccg gcgaggtcga ggtccgtccc
    15541 cgtgccgacg gacccgtgac cacggtgttc gtgcgactcc tcggcaccga cgacgcctgg
    15601 tcggtcctgg gaacggccac gcccaacatc gaggtcgacc aaccgacggc gctcaccacg
    15661 atcgggaacc cggtccgtct gatcggtcga gcccgggcct tcgaaggcac cgtggacgtc
    15721 gaggtccgcg aggacggcac caccacacca ctcggcaccg gattcgtgac cggacggggc
    15781 gacggcgtcc tcgggccctt cgactccgaa ctgccctacg aggtggccgg caccgagcgc
    15841 ggcactgtgc tcttcgtcac ccacagcgcc gaggacggca gcgtgtggga agcagccgtc
    15901 atccgcgtcg tcttcccaac accctgacca cggaggtcgc ccgatggacc gactctcggc
    15961 gctcgacacc gagttcctac acctcgagga cgacacctca ccgatgcaca tcgcatcggt
    16021 ctgcgtgttc gccggaccgg cgcccagcct caccgagctg accgacctcc tggagtccaa
    16081 actccacgag ctgccccggt accgccaggt gatccgcacg gtcccattgg agttgggccg
    16141 acccatctgg gtcgacgacc ggcgattccg gctcgactac cacgtgcacc ggaccgcgct
    16201 gccgtcgccg ggaggcgccg ccgagctcga cgatctcatg ggccgcctca tggcccaccc
    16261 gctcaaccgg tcgcgtcccc tctgggaggc gtggctggtg gagggcctga gcgacgaccg
    16321 ttgggcgctg gtgttcaagg tgcaccactg catggtggac ggcgttgcgg gcgtccacct
    16381 cctcgaagcg ttcctcgacg tctcgcccga cacgccgccg ggcaccgtgc ggccgtggcg
    16441 ggcacaaccg gaaccgtcgc cgctgggcaa agtcgtcggc tcgtgggtcg gtctcgccgg
    16501 tgacgtgatc ccgtgggggc ggcggatcgc caccgcggct tcgtcgccga tcgacgcggt
    16561 ccgtgacgtg gccgccatca gcaccggact cggcagcttc ctcgcgcacc tcgtccccgc
    16621 gccgggcaac gagctgcagg gccgcattgg tccccatcgc cgatggtccc acgtcacgct
    16681 cgacctcagt gacgtggccc ttgtccgccg acgcctcggc ggtacggtca acgacgtgct
    16741 cctcgcctgc gtgacggccg ggcatcgggc cctgctcgaa gcacacggcg acgacccgtc
    16801 gaccgcggtc gtccgcaccg cgatcccggt ctcgacccgc gactggggca cgagcgggag
    16861 cggcaaccgg gtgtcgaccc tgctcgccga gctgcccgtc ggccagcccg accccctcgt
    16921 ccgtctcgcc accgtgcgcg agttcacgac ccggctgaag gcatcgagca tggcgtccgt
    16981 cgccgacacg ttcttcacgc tcgcagacct cgcccctccc ctcgtggtcg gcccgatcag
    17041 ccggctcgcc atccgcctcg agcaccgacg ggcgcaacgc tcgatcacca cgatcaccac
    17101 caacgtgcca ggccccccgt tcccgctgta ctgcctcggt cgcgagatga tcgagcagct
    17161 gccgtacgtc gggctcaccg acggggtgcg ggtcacgacc gccatcgtct cgtacaacgg
    17221 tcacatcacg gtgggcgtca ccggcgacga ggactcggtg cccgatgtgg gcgtcgcgac
    17281 cgcggcgatc ggcgacgcgc tcgcagagct ggtgcgcctg gcccgacgtc gcaaggatcg
    17341 cacgcggtga ccgacacgat gcgcgcctgg gtcgtcgacc ggcccggacc cgtcgcgacg
    17401 ggcgcgccgc tcgtggaagt cgagcggccg ctcccggtcc ccgccgccgg ccaggtacgc
    17461 gtgcgggtgt cgtgctgtgg tgtctgccga accgacctcc acctcgccga gggggacctg
    17521 tctccccacg ctcgccgcgt cgtgccgggc cacgagatcg tcggcgtcgt cgacgcactc
    17581 gggtccgaca gccgtcgctt cgccctcggc gaacgcatcg gcatcgcgtg gctcgcgacg
    17641 acgtgccgga cctgtcgctt ctgccgatcg gcgcgggaga acctctgcac cgctccgacg
    17701 ttcacggggt gggaccacga cggcgggtac gccgagttcg ccctcgtcga cgaagggtac
    17761 gcctaccggc tcccggacgg gttcgacgac ctcacggccg cgcccctcct gtgcgccggc
    17821 atcatcggat accgagcgct cgcacgggcc gacctgccgc ccggtggtcg actcggtatc
    17881 tacggcttcg gagcgtcggc ccacctggcg gcccaggtcg cgatcgccgg cggcgccgag
    17941 gtgcacgtca tgacccgcag tccggccgcc cgtgcgttgg cgatgcgtct cggcgcggcg
    18001 agcgcgcagg gagcagccga cccaccgccg gtgcccctcg acgcgtcgat cctgttcgcc
    18061 ccggtcggca ccctggttcc ggcggcactc gaaggactcg accgcggcgg caccctgtcg
    18121 atcgccggga tccacctgtc cgacatcccg tcgctcgact atcaacggca cctgttcgag
    18181 gagcggaccc tccgcagcgt gacggccaac acccgcgagg acggcgagcg cttcctcgcg
    18241 gtggccgccg acatcggcat ctccgtcgaa acgaccgagt accccttcga cgaggcggac
    18301 acggcgctgg ccgacctcgc ggccgatcgg gtggacggcg cggccgtgtt gcgaatgccg
    18361 tcagcgcgct gaggcgagcg cggtgttcac aactcgggat cggccggcgg gaccaccacc
    18421 gccggacacg gggcgtgcac ggccacc
//
LOCUS       DAOSOA010000017        18100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3111_length_18100_cov_5.703408, whole genome shotgun sequence.
ACCESSION   DAOSOA010000017 DAOSOA010000000
VERSION     DAOSOA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 18100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18100
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3111_length_18100_cov_5.703408"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKY13_02475"
     CDS             complement(<1..296)
                     /locus_tag="PKY13_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78819.1"
                     /translation="MTFLRTSFAVLATAIIGSLLTVVSTATPTAADDNPSTSALRSSC
                     DIDRTVSTGTAAGLPAVAPGERLTVSSRLVLDAGQAYQDVVGRPDGSTYFASVI"
     gene            complement(491..811)
                     /locus_tag="PKY13_02480"
     CDS             complement(491..811)
                     /locus_tag="PKY13_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78820.1"
                     /translation="MRHVQLTVATVALGPRDLPLRHGTLQVVERDAGPLDWELVLHTI
                     EFEPVANAVHPLTIAAITGADDHGRLITSDVEGEALLVRGVEHSIVFRGHSDLRGFDR
                     DLLV"
     gene            836..1207
                     /locus_tag="PKY13_02485"
     CDS             836..1207
                     /locus_tag="PKY13_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78821.1"
                     /translation="MITTISLRDIHTDWAWVVIVSNGLVGLWSTGAYWLAPLRLRSMW
                     WLVVAAEVAIFVQVGMGVWLLSVQNIPAPDFHTFYGFVTLISVMILFAYRNQLRAHLY
                     LLYGLGSLFLMGLGLRAVVLG"
     assembly_gap    1208..1307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1308..2848)
                     /locus_tag="PKY13_02490"
     CDS             complement(<1308..2848)
                     /locus_tag="PKY13_02490"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOT78822.1"
                     /translation="MPGTTRNQELLDWVEQWRSIFEPDDVYWCDGSAEEYERLCQELV
                     DAGVFEKLSDAKRPNSYLALSDPNDVARVEDRTFIASEQEIDAGPTNHWREPAALMAE
                     MMEQYRGVMKGRTMYVVPFSMGPLGSPIAHIGVQLTDSAYATVNMRIMTRMGQGALDV
                     LGDEGEFVPAVHSVGYPLVLADGTTRPDVAWPCNETKYISHFPETREIWSFGSGYGGN
                     ALLGKKCFALRIASTMARDEGWMAEHMLILGLTPPNGEKRYVAAAFPSACGKTNMAML
                     IPSLPGWKVETVGDDIAWMKFGPDGRLYAINPEAGFFGVAPGTNDATNRNAMASIESN
                     TIFTNVAKTDDGDVWWEDMTPEAPAHLIDWKGNDWTPESGTPAAHPNARFTAPAAQCP
                     AIAPEWEDPKGVPISAILFGGRRATNVPLVTEAFNWNHGVFLGSIMSSEKTAAAAGAI
                     GDVRFDPFAMLPFMGYNVGDYIKHWLEIGEATDSSNLPKMFWVNWFRKDENGKFMWPG
                     YGENSR"
     gene            complement(3057..3320)
                     /locus_tag="PKY13_02495"
     CDS             complement(3057..3320)
                     /locus_tag="PKY13_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOT78823.1"
                     /translation="MQGVIKAFDPGTGDGVILCDADLADYDLHPDALLGSVFRMLRQG
                     QRVTFELTDDGLATQLRLGSEADMGTPGFHETLPSADPVVDPN"
     gene            complement(3328..3534)
                     /gene="rpmB"
                     /locus_tag="PKY13_02500"
     CDS             complement(3328..3534)
                     /gene="rpmB"
                     /locus_tag="PKY13_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT78824.1"
                     /translation="MSSVCEICNKKPGFGMSVSHSHRRTKRRWNPNIQRVRAVVNGAP
                     KRVNVCTGCLKSGKVQKPVARVLP"
     gene            3714..4661
                     /locus_tag="PKY13_02505"
     CDS             3714..4661
                     /locus_tag="PKY13_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOT78825.1"
                     /translation="MDPLTQLRADDVVDVLVAAQESMLAHAPALDRLDRLDQLEGIDG
                     GDTGTHLAATLGAVVAGLTPGVTLTQLATDLQSVATSAGVGRSGRMLGAFLGGFAELC
                     RNADALDPVRWAMAFEAGSDAIAEATADVRPGALPSVAEVLAAVALRGADDGMALPEL
                     VIEVADRGLDALERTPELWAPLAEAGVVDAGGAGLLVLVDAFVAVIHGDDPELPEWEF
                     PEDADDDGEVFADERFRYEITLSLSSPASPGADPATAVDELGKRLQRAWISLGDEVQL
                     LTADGRLTASVCSDDIGAVIEAAVGLGRPSGIVVRDRRG"
     gene            4654..6819
                     /gene="recG"
                     /locus_tag="PKY13_02510"
     CDS             4654..6819
                     /gene="recG"
                     /locus_tag="PKY13_02510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOT78826.1"
                     /translation="MASSAPPDPTPITLADLAAIPVERLSGVGPKKLDGLHKLGIDSL
                     LDLLTHYPRRYLDRTNQAKVRDLRPGEEGMVLATVRSISSRRVKGGRAMVTGDIADET
                     GRLKISFFNQGWREKQLPVGTEAIFYGKVEDFRGVRQMTNPVVDLIGNRTGKIIPVYP
                     QSEKSGLSTWDLGDMCAQALRRCEPRGIAEPLPAGILERFDFVDRQTALIGIHTPESM
                     GQSAAARKRLVFDELLRIQLALVLRKRALELTSVGIVHDPSTALMSTYWQRLPFPLTG
                     AQQRVIGEIIADLARPIPMHRLLQGDVGAGKTVVAVSALLAAVQGGHQGAMMAPTEVL
                     AEQHALGLRRDLAGFQVPAEEGSLFEDRELRIALLTNRVTGAERKQILADLADGGVDI
                     VVGTHALIQDAVKFRSLGVVVIDEQHRFGVEQRAALRSSNPDGTAPDVLVMTATPIPR
                     TAAMTVYGDLDVSVLDELPPGRTPIETSWARSESDVDDAWKLVRGEVEQGRQCYVVCP
                     LIEESQKLEVSSATETFEQLSAGELYGLRLALLHGRMTSQEKEATMTAFRRGDLDVLV
                     ATTVIEVGVDVPNATVMVILDADRFGIAQLHQLRGRVGRGAHRSFCVLVAGEPTPDGE
                     ARLEAMVRTTDGFDLAEVDLDLRGEGTIMGERQKGRNDLRLASLRRDRPWVERAREVA
                     FELIDDDAGPGDGLHHLPALRDELKLFLGEEDEEFLLKG"
     gene            6868..7491
                     /gene="rsmD"
                     /locus_tag="PKY13_02515"
     CDS             6868..7491
                     /gene="rsmD"
                     /locus_tag="PKY13_02515"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOT78827.1"
                     /translation="MCGFARAARRVSPRSASRRWASTVLPMRVVAGTARGRTIDAPPG
                     TSTRPTSDRVRESIFNALGSLDVVVDATVLDLFAGSGALGIEALSRGAARATFVESDR
                     RAARVIEENLRRCGLDDRATVVTADATRFALRGSSFDLALADPPYAFDQWPDLLDAVD
                     AETLVIESDRAVAVTDRWDVVRDRTYGSTVVQIVTRAHPNPPVAPRS"
     gene            7496..7975
                     /gene="coaD"
                     /locus_tag="PKY13_02520"
     CDS             7496..7975
                     /gene="coaD"
                     /locus_tag="PKY13_02520"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT78828.1"
                     /translation="MTVVLYPGSFDPVHNGHVEIVETAARLFDKVVVAAMRNPQKGEP
                     LFSLEDRQALIEEAFAHLDNVEVTMFSSLVVDLARNVGAHFIVKGLRAVSDFESELQM
                     AQMNRSISGVDTLFIPTTSSHSFLASKLIRDIARFGGDVTSMVPPCVAAKLKEQYPS"
     gene            7972..8574
                     /locus_tag="PKY13_02525"
     CDS             7972..8574
                     /locus_tag="PKY13_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78829.1"
                     /translation="MSRPIEDGVDDSSTTTDTLPTYRSEDQLRQLVEIVEVARSVPMS
                     STVMVHREEVLDILDDVLAHLPDELRAARWLLKEREEFRAKAHREAEEIIADAKSHVA
                     RMVQRTEVVKAANQHAQQILDDAEQESRRMMREAEDYCDQKLASFEVVLDRLSKTVAG
                     GRRKLAGTTIAEAELGHRTAGVYDDDDSSEAAAFFDQELD"
     gene            8606..9115
                     /locus_tag="PKY13_02530"
     CDS             8606..9115
                     /locus_tag="PKY13_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HOT78830.1"
                     /translation="MTGGNPLLINVVELRRSVGNQKTVSESVELDDVGLASVRVEVGS
                     ALQVDLLLETLSGGIAATGTVTGRWNGECRRCLEKVEGPLDVEVAEVFEEHPTEGETY
                     PISGDHIDLEPMVRELAILGLPINPLCRDDCAGPVPDVLPVSVESDEEAEPAPDPRWA
                     ALDALRDLT"
     gene            9236..9415
                     /gene="rpmF"
                     /locus_tag="PKY13_02535"
     CDS             9236..9415
                     /gene="rpmF"
                     /locus_tag="PKY13_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT78831.1"
                     /translation="MAVPKKKMSKSKTRMRRASAWKLSAPSRSLCPRCGVSKLPHVVC
                     GNCGWYHGRQAIEVD"
     gene            9575..9865
                     /locus_tag="PKY13_02540"
     CDS             9575..9865
                     /locus_tag="PKY13_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT78832.1"
                     /translation="MPAETHTHFASLDRQRIVEIVQERLGEILEIEPSTIKETDSLVD
                     DLHADSLAQYQLVEALVDEFGERTVGPGIEVEDLEDLELVSDAVDYVVARLA"
     gene            9843..10583
                     /gene="rnc"
                     /locus_tag="PKY13_02545"
     CDS             9843..10583
                     /gene="rnc"
                     /locus_tag="PKY13_02545"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOT78833.1"
                     /translation="MSSPDSLEPGRLVLLGDLSRRLGHDFSGLSLLDAALRHRSWCGE
                     HGAVPSNERLEFLGDAVLQLAITERLFAADPPLPEGVLAQRRAALVNTRTLANAARRV
                     DLGPAIRLGRGEAATGGHDKDSILADSAEAVIGAVHLDAGFDAARAVIFSLLEPDLER
                     VEAGKDTGDSKSRLQERAAHELDLVPRYEVSGAGPDHAREFEAAVLLDGVEWGRGEGR
                     TKKEAEQVAAAAALQRLVDEGKLTDEHG"
     gene            10576..11403
                     /locus_tag="PKY13_02550"
     CDS             10576..11403
                     /locus_tag="PKY13_02550"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOT78834.1"
                     /translation="MAELPELEILRRDLDKDVVGKKIKTVEAPSAATIKRAGTKKSLI
                     SRLEGAKIDSVSRKGTWILLGLDTGEVLMIGLGDRAQLQRNQNKDAVAKGTAVVLTFT
                     QHGQLRLVDPAKSSELFVVTAEEVDDIVGPLGLDLVDEPVSWTKFGEMVLRRGGKLKG
                     ILMDPTMLVGIGPMYSDEILFEAGLRYDRLADKLSTQEIRRLYRASVEIVHDAVKHGG
                     ATVGADGFLTLSGEPGGYGPMINVYGRDGEMSPRARGPIVKAKFGSGFTYYCEQTQV"
     gene            11610..15098
                     /gene="smc"
                     /locus_tag="PKY13_02555"
     CDS             11610..15098
                     /gene="smc"
                     /locus_tag="PKY13_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOT78835.1"
                     /translation="MFLKTLQIKGFKSFADVATLQLEPGVTVVVGPNGSGKSNVVDAI
                     GWVLGAQAPSAVRSQKMDDVIFAGSAKRPALGRAEVGLTIDNSSGVLPIEFNEVTITR
                     TLFRSGDSEYAINGVNCRLLDIQELLSDSGVGRQQHVIISQGQIDAVLNAKAEERRLI
                     IEEAAGVLKYRRRKEKSERRLNATEANLLRIQDLLREVRRQLRPLERQADAARRHDAV
                     ITELAALKLHLVGRELARLRTQLAESTTRQNELRNADRELRRVLAGLDAEVLATEAQL
                     SSMGGDDFGDALVRYESLRERARGLQSLLTERRRGIDRDRNAFVDQAVVASLEAEHAQ
                     LSAELREVESEGVGAATGSGALARAEAELAEARAAFEADWGDGVAAPPSEAAEIRGEL
                     AALRATMERTESDNRRLSNQRETLVTKVERLAAEFERIGGERAAAHDNVESLARQAAE
                     AEAERAAAEEAIVEIEARATAAAAEHHTVAARAEALALALDGARQRAGAAHLAELDGV
                     VGTLLDVVQVEAGWESAFEAAAGEALASVVVADAEVARRALEALAGGELSGAVLAISH
                     AVAQAPRVAVPVGVGRPVRDLVKATRADVEPLLDALLAGAVVIEGEWAEAVDVALAHP
                     DATIITRSGDRFGTTGWRIGQSSAGATGAALAEAEEQLGSARVAAEDTALELARAKER
                     LSTARRDANDAEKANDQNANRVRTLDDVLRRAERDRTEAEAELVTVDGHLNELAQRIE
                     REVTRRTDLEQRLPALEAVESELSERARTMAEARRNLEAKAGEVGALRTDVEVKVAGV
                     EERRRFLTDRLAQVDERLSRNQAERLAAEQKRLELDARAEVTDTLITFVADRLGAVES
                     VLEVLREARRRQSEKTRAVSAQLEALRRQRSTTEKELEEHRERIQRSEISDAETRLRL
                     ETATESLRSELDSEPDVAIAAECPELPDGMSPQARLRELEREVRLMGPINPLALQEFE
                     ALQERHQFLEAQLEDIRATRKELSKVIKAVDEEIVNVFASAYADVSQNFQALFDMLFP
                     GGQGGLTLTDPSNLLETGIEIEARPSGKNVRKLSLLSGGERSLVALAYLFAVFRSRPS
                     PFYVMDEVEAALDDVNLHRFLSLVAEFRSTAQLLIVSHQKRTMEAADVLYGVTMEPGG
                     SSRVISEKVGAASGGS"
     gene            complement(15120..15509)
                     /locus_tag="PKY13_02560"
     CDS             complement(15120..15509)
                     /locus_tag="PKY13_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78836.1"
                     /translation="MRPFTPPRVLAVALASAFLLSGCGGDEAFPKDEFVKQTTASGIT
                     KPVAECAYDQIKDNAAINKELDRAGGPNPNISEKVSGELSTILARCLLAADEAANPTT
                     TTAKPTSKSTTTEKTSSSDKTTTTKKK"
     gene            complement(15545..16516)
                     /locus_tag="PKY13_02565"
     CDS             complement(15545..16516)
                     /locus_tag="PKY13_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT78837.1"
                     /translation="MNWSFISPGRLWWLIAVAAMAAAYIAMQFRRTKYAVRFSNMDLL
                     DKVAPKRPSWRRHVVASVYLLALAAMVVGVAQPQAQERVPKERATIILAIDTSLSMQA
                     TDVSPTRIDAAKVAAVKFVESMPPKVRLGVVDFSGSTKLLVPPTTQRDTAVRAIKNLE
                     LGQGTAIGAAIDTSLTAIANSVPSEGDTKIPAVIVLISDGKDQGQSLPVPTAIERANK
                     AKVPVFTIAYGTPNGVVEIDDGSGSTQLVPVPVDEPALAEVAKETGGESFAAATAGEL
                     EQVYDRLGSAVGYDIEQQEVTWKLVAGALIALALSGAMSLVWFQRLP"
     gene            complement(16604..17581)
                     /locus_tag="PKY13_02570"
     CDS             complement(16604..17581)
                     /locus_tag="PKY13_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT78838.1"
                     /translation="MAKRSSPDPAGGTDVVGPGAGPMGDRRAADVLRRLELDVTRRLD
                     GILHGDHRGLVPGHGSEPGEAREYQAGDDVRRIDWNVTARMSTPHIRESIADRELETW
                     IVADRSASLDFGTVTWEKRDLVLAAAAGVGFLTSRGGNRIGAVISGSHGAVTVPARQG
                     RKHLLSILHQIQGAPRADAAPAADLAATLKRAASVSRRRSLVVVISDWLEDPETWRHA
                     LATLSVRHEVLAVEVLDPRELDLPKMGVLTLIDTESGRTREIDTNNRGLRERYAAAAA
                     EQRSDIRLAIAGSGAHHLQLRTDRDWLLDLARHVASTKRRQANVGGAHR"
     gene            complement(17585..>18100)
                     /locus_tag="PKY13_02575"
     CDS             complement(17585..>18100)
                     /locus_tag="PKY13_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT78839.1"
                     /translation="LGYPTPHEELEIVNRMGVSPPQPAEVVSLADVLRLQAAADACYC
                     DRSVVEYAVNLVLATRTPQNYGLADLAPFLEFGASPRASLGLVRAGRAMALLRGRNYV
                     VPQDIFDVAPEIMRHRLVLTYEALAADVDAEQIMIRVLSTVPAPVIQPSQIAGPNDAY
                     YRSGTDSQAWG"
BASE COUNT         2724 a         6082 c         6333 g         2861 t
ORIGIN      
        1 tcgatgaccg aggcgaagta ggtcgatccg tcgggccggc cgacgacgtc ctggtacgcc
       61 tgcccggcgt cgaggacgag ccgtgagctc accgtcaggc gttcgcccgg tgccaccgcg
      121 ggcagtcctg cggcggtgcc ggttgacacc gtccggtcga tgtcgcagct cgagcgcagc
      181 gcgctcgtcg acgggttgtc gtcggcggcc gttggagtcg cggtgctgac aaccgtcagc
      241 agcgagccga tgatggccgt ggcaagcacg gcgaacgagg ttcggaggaa cgtcatgtcc
      301 cgtccgtcgg ccgatcccgc cgtcgccctg atgcggttcg agccgaaagt gcccgcgctg
      361 ggaagtattg cccagaccga cccgggatga tctcgacatc gacgtgggtg cggtcgcccg
      421 gatcgagcca tcgaggccgt tcgggcgatg ttcgggcccg tcccggtgcc ggccggacac
      481 cgcctcgttc tcagacgagc aggtcgcggt cgaagcctcg caggtcgctg tggccgcgga
      541 agacgatcga gtgctcgacc ccgcgcacga gcagggcctc gccctcgacg tcactcgtga
      601 tcagccgacc gtggtcgtcg gctccggtga ttgccgcgat cgtcagggga tgcacggcgt
      661 tcgcgaccgg ttcgaactcg atggtgtgca gcacgagttc ccagtcgagc gggccggcgt
      721 cgcgctcgac cacctgcagc gttccgtggc gcagcgggag gtcccggggg ccgagcgcga
      781 ccgtggcgac ggtgagttgg acgtggcgca tcggtggtga aagtacattg cgacggtgat
      841 cacgaccatc tccttgcggg acatccacac cgattgggcg tgggtggtga tcgtgtcgaa
      901 cggcctcgtg gggctgtggt ccaccggcgc ctactggctg gcgccgctcc gcctgcggtc
      961 gatgtggtgg ctcgtggtcg ccgcggaggt tgccatcttc gtccaggtgg gcatgggggt
     1021 atggctgttg tcggtccaga acattccggc acccgacttt cacacgttct acggcttcgt
     1081 gaccctcatc tcggtgatga tcctcttcgc ttaccgcaat cagctgcggg cccacctgta
     1141 cctgttgtac ggcctcggca gcctcttcct gatggggctc ggcctgcgcg cggtcgtctt
     1201 gggctgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg ctgttctcgc
     1321 cgtagcccgg ccacatgaac ttgccgttct cgtccttgcg gaaccagttg acccagaaca
     1381 tcttcggcag gttcgaggag tcggtggcct caccgatctc gagccagtgc ttgatgtagt
     1441 cgccgacgtt gtagcccatg aagggcagca tcgcgaacgg gtcgaagcgc acgtcgccga
     1501 tggcgccggc agcggcggcg gtcttctcgg aactcatgat cgagccgagg aagacgccgt
     1561 ggttccagtt gaacgcctcg gtcacgagcg ggacgttggt ggcgcggcgg ccgccgaaga
     1621 ggatggccga gatcggcaca cccttcgggt cctcccactc gggggcaatg gcggggcact
     1681 gcgccgccgg ggcggtgaag cgggcgttcg ggtgggcggc gggggtgccg gactcggggg
     1741 tccagtcgtt gcccttccag tcgatgaggt gcgccggcgc ctcgggcgtc atgtcctccc
     1801 accacacgtc gccgtcgtcg gtcttggcga cgttggtgaa gatggtgttc gactcgatcg
     1861 acgccatggc gttgcggttg gtggcgtcgt tggtgccggg cgccacgccg aagaagccgg
     1921 cctcggggtt gatggcgtag agccgcccgt cggggccgaa cttcatccag gcgatgtcgt
     1981 cgccgacggt ctcgaccttc cagcccggga ggctggggat gagcatcgcc atgttggtct
     2041 tgccacaggc agacgggaag gcggcggcga cgtagcgctt ctcgccgttc ggcggggtga
     2101 gccccaagat gagcatgtgc tcggccatcc agccctcgtc gcgggccatc gtcgacgcga
     2161 tgcggagggc gaagcacttc ttgccgagga gggcgttgcc gccgtagccg gagccgaacg
     2221 accagatctc acgggtctcg gggaagtggc tgatgtactt cgtctcgttg cagggccagg
     2281 cgacgtcggg gcgggtggtg ccgtcggcga ggacgagcgg gtagccgacc gagtgcacgg
     2341 cggggacgaa ctcgccctcg tcgcccaaga cgtcgagggc gccctggccc atgcgggtca
     2401 tgatgcgcat gttgaccgtg gcgtaggccg agtcggtgag ctgcacaccg atgtgggcga
     2461 tgggggagcc gagcgggccc atcgagaagg gcaccacgta catggtgcgg cccttcatca
     2521 ccccgcggta ctgctccatc atctcggcca tgagggcggc cggctcacgc cagtggttgg
     2581 tggggcccgc atcgatctcc tgctcggagg cgatgaaggt gcggtcctcg acgcgggcga
     2641 cgtcgttggg atcggagagc gccaggtagc tgttgggacg cttggcgtcg ctcagcttct
     2701 cgaacacacc ggcatccacg agctcctggc agagacgctc gtactcctcg gcggacccgt
     2761 cgcaccagta gacgtcgtcg ggctcgaaga tcgagcgcca ctgctcgacc cagtcgagca
     2821 gctcctgatt gcgggtcgtt cctggcatgg ttcctccggt ttcgccaccg aattgttcct
     2881 gccggcggca ggtctggatt cgtattgctt aaagaagtcg ttcgagtgac gcaacggcca
     2941 atcgaggtat ctccgaccat gtggggggtt cccacgacgt tcgagtgaac agtctgaccg
     3001 gtactgcgta catctcacct atcgggggac gcccggcgac agggaccaaa gtcccgtcag
     3061 ttcggatcga ccaccgggtc ggcgctcgga agggtctcat ggaaccccgg agtgcccatg
     3121 tctgcctcgg aaccgagccg gagctgggtg gcgaggccgt cgtcggtcag ctcgaacgtg
     3181 acgcgctggc cttggcgcag catacggaac accgacccca ggagggcgtc ggggtgaagg
     3241 tcgtagtcgg ccagatcggc atcacagagg atgacaccgt cgccggtgcc gggatcgaag
     3301 gccttgatga cgccctgcat gacgtgccta gggaaggacg cgggcgacgg gcttctggac
     3361 cttgccggac ttgaggcagc ccgtgcacac gttgacccgc ttgggcgcac cgttgaccac
     3421 ggcgcggacc cgctggatgt tcgggttcca acgacgcttc gtgcggcggt gggagtggct
     3481 gacactcatg ccgaatcccg gcttcttgtt gcagatctcg cagacggagg acatggttcg
     3541 gcacctgatc gttcgtgggg atggaggctg gttggatgca ccgagggcgc ggccctcggc
     3601 gggctctcca aacgctccgg cgatgttagc agcccccaac gggcgccccc caatcatgcc
     3661 ggccaccgtc ggcaaccagt gggtgtcacc gtgagcgggt agcttcccag ccgatggatc
     3721 cactgaccca attgcgggcc gacgacgtgg tcgacgtgct ggtcgcggcg caggagtcga
     3781 tgctcgccca cgccccggcg ctcgatcgac tcgaccgcct cgaccagctg gagggcatcg
     3841 acggcggtga caccggcacg catctcgccg ccacgctcgg cgccgtggtc gccggcctca
     3901 cccccggcgt cacgctcacc caactcgcca ccgacctgca gtcggtcgca acgagcgccg
     3961 gggtgggccg ctcgggtcgc atgctggggg cgtttctcgg cgggttcgcc gagctctgcc
     4021 gcaacgccga tgcgctcgac ccggtgcggt gggcgatggc gttcgaggcc ggctccgacg
     4081 ccattgccga ggccacggcc gacgtccgcc ccggggccct gccgtcggtg gccgaggtgt
     4141 tggcggcggt ggcgctgcgt ggggccgacg acggtatggc gcttcccgag ctcgtcatcg
     4201 aggtggccga ccgaggcctc gacgcgttgg agcgtacgcc cgagctgtgg gcgcccctcg
     4261 ccgaggccgg ggtggtcgac gccggcggtg ccggcctgct cgtgctcgtc gacgcgttcg
     4321 tcgcggtcat ccacggtgac gaccccgagc tccccgagtg ggagttcccc gaggacgccg
     4381 acgacgacgg tgaggtgttc gccgacgaac ggttccgcta cgagatcacg ctgtcgctgt
     4441 cctccccagc gtctcccggc gccgacccgg cgaccgccgt cgacgagctc ggcaagcgcc
     4501 tgcagcgcgc gtggatcagc ctcggtgacg aggtgcagct gctcacggcc gacggtcgcc
     4561 tgacggcgtc ggtctgcagc gacgacatcg gtgccgtcat cgaggcggcc gtcgggctcg
     4621 ggcgaccctc gggaatcgtg gtgcgcgatc gccgtggcta gttccgcccc gcccgacccc
     4681 acgccgatca cgctcgccga tctggccgcc atccccgtcg agcggctgag cggagtcggc
     4741 ccgaagaagc tcgacggtct ccacaagttg ggcatcgact cgctcctcga cctcctcacg
     4801 cactacccga ggcgatacct cgaccgcacc aaccaggcca aggtgcgaga tctccgcccg
     4861 ggcgaggagg gcatggtgct cgcgaccgtt cggtcgatct cgtcgcggcg ggtcaagggc
     4921 ggtcgcgcca tggtcaccgg cgacatcgcc gacgagacgg gccggctcaa gatctccttc
     4981 ttcaaccagg gctggcgcga gaagcagctg cccgtcggca ccgaagccat cttctacggc
     5041 aaggtcgagg acttccgggg cgtgcgccag atgacgaatc ccgtcgtcga cctgatcggc
     5101 aaccgcaccg gcaagatcat tccggtctat ccccagagcg agaagtcggg gctgagcacg
     5161 tgggacctcg gtgacatgtg cgcccaggcc ctccgacggt gcgaacctcg cgggatcgcc
     5221 gagccgctgc cggcggggat cctcgagcgg ttcgacttcg tcgatcggca gacggcgctc
     5281 atcggcatcc acacccccga gtccatggga cagtcggctg cggcccgcaa gcgtctcgtg
     5341 ttcgacgagt tgctgcgcat ccagctggcg ctggtgcttc gcaagcgggc gctcgagctc
     5401 acctccgtcg gcatcgtcca cgacccctcg accgcgctga tgtcgaccta ctggcagcgg
     5461 ctgccgttcc cgctcaccgg tgcccagcag cgggtgatcg gcgagatcat cgccgacctc
     5521 gcccgcccga tccccatgca ccggctgctg cagggcgacg tcggcgccgg caagacggtc
     5581 gtggcggtga gcgcattgct ggcggccgtc cagggcgggc accagggggc gatgatggcg
     5641 ccgaccgagg tgctggccga gcaacacgcg ctggggttgc ggcgcgatct cgccgggttc
     5701 caggtgcccg ccgaggaggg ttcgctgttc gaggaccgcg agctgcgcat cgccctgctg
     5761 accaaccggg tcacgggtgc cgaacgcaag cagatcctgg ccgacctcgc cgacggtggc
     5821 gtcgacatcg tggtcggcac ccacgcgctc atccaggacg cggtgaagtt ccgttcgctc
     5881 ggcgtcgtgg tgatcgacga gcagcaccgg ttcggtgtcg agcagcgtgc tgcgctccga
     5941 agctcgaatc ccgacggcac ggcacccgac gtgctggtga tgaccgcgac cccgatcccg
     6001 cggacggcgg ccatgacggt ctacggcgac ctcgatgtgt cggtcctcga cgagctgccc
     6061 ccgggccgca cgcccatcga gaccagttgg gcgcggtcgg agtccgacgt cgacgacgcg
     6121 tggaagctcg tccggggcga ggtggagcag ggccggcagt gctacgtggt ctgcccgctc
     6181 atcgaggagt cccagaagct cgaggtgtcg tcggcgaccg agaccttcga gcagctgtcg
     6241 gccggcgagc tctacggcct gcggttggcg ttgctgcacg gccgtatgac gtcgcaggag
     6301 aaggaggcca cgatgacggc cttccgtcgc ggcgacctcg atgtgctggt ggccacgacg
     6361 gtgatcgagg tcggcgtcga cgtgcccaac gccaccgtca tggtcatcct cgacgccgac
     6421 cggttcggca tcgcccagct ccaccagctg cggggccgcg tcgggcgtgg tgcccaccgg
     6481 tcgttctgtg tgctcgtcgc gggggagccc acgcccgacg gcgaggcccg cctcgaggcc
     6541 atggtgcgca cgaccgacgg gttcgacctg gccgaggtcg acctcgatct gcgtggcgaa
     6601 ggcacgatca tgggggagcg gcagaagggt cgcaacgacc tgcggctggc ctcgctccgc
     6661 cgtgaccggc cctgggtgga acgtgcccgc gaggtcgcgt tcgagctcat cgacgacgac
     6721 gccgggcccg gcgacgggct gcaccatctc ccggccctcc gtgacgagct gaagctgttc
     6781 ctgggcgagg aagacgaaga gttcctcctc aaggggtgag tgcgccgtcg ggcgaccgac
     6841 gacacctccc gccaaacgcg tcgcgagatg tgcggtttcg cgagagcggc gcgacgcgtt
     6901 tcaccacgtt cggccagtcg acggtgggcg agtaccgtgc tgcccatgcg cgtggtggcc
     6961 gggacggctc ggggacggac gatcgacgcc ccgccgggca cgagcacccg cccgacctcc
     7021 gaccgggttc gcgagtcgat cttcaacgcc ctcgggtcgc tcgacgtcgt cgtcgacgcc
     7081 accgtgctcg acctgttcgc cggttccgga gcgctcggca tcgaggccct gtcgcgcggt
     7141 gccgcgaggg caacgttcgt cgagtcggac cgccgcgccg cgcgggtcat cgaggagaac
     7201 cttcgccggt gcggcctcga cgaccgggcg acggtcgtga ccgccgacgc gacgcggttc
     7261 gccctccgcg gctcgagctt cgacctggcc ctcgccgacc cgccgtacgc gttcgaccag
     7321 tggcccgacc tcctcgacgc cgtcgacgcc gagaccctcg tgatcgagtc cgatcgcgcc
     7381 gtcgcggtca ccgaccgatg ggatgtcgtg agggaccgca cgtacggaag tacggtggtg
     7441 cagatcgtga cccgggcgca cccgaacccg cccgtagccc ccaggagttg atgcagtgac
     7501 cgtggttctc tatccgggtt cgttcgaccc cgttcacaac ggacacgtcg agatcgtcga
     7561 gaccgcggcc cggctgttcg acaaggtcgt ggtcgcggcg atgcgcaacc cgcagaaggg
     7621 tgagccgctg ttctccctcg aggaccgaca ggccctcatc gaagaggcgt tcgcccacct
     7681 cgacaacgtc gaggtcacga tgttcagttc cctcgtcgtc gacctcgccc gcaacgtcgg
     7741 cgcacatttc atcgtgaagg gcctgcgggc ggtctccgac ttcgagtcgg agttgcagat
     7801 ggcgcagatg aaccgctcca tctccggggt cgacaccttg ttcatcccga cgacctcgtc
     7861 ccactcgttc ctcgcgtcca agctcattcg cgacatcgcc cgcttcggcg gcgacgtcac
     7921 ctcgatggtc ccgccgtgcg tggcggcgaa gttgaaggag cagtacccct catgagccga
     7981 ccgatcgagg acggcgtgga cgactcgtcg acgacgaccg acacgcttcc gacctaccgc
     8041 tccgaggatc agctccgcca gctggtggag atcgtcgagg tggcccggtc ggtgcccatg
     8101 tcgtcgacgg tcatggtgca tcgcgaggaa gtcctcgaca tcctcgacga cgtactcgcc
     8161 cacctccccg acgagctacg ggcggcccgc tggttgctca aggagcgcga ggagttccgc
     8221 gccaaggcac accgtgaggc cgaggagatc atcgccgacg cgaagtccca cgtcgctcga
     8281 atggtgcagc gcaccgaggt cgtgaaggcg gcgaaccagc acgctcagca gatcctcgac
     8341 gacgccgagc aggagtcgcg tcgcatgatg cgagaggccg aggactactg cgaccagaag
     8401 ctcgcgagct tcgaggtggt cctcgaccgc ctgtcgaaga cggtcgctgg tggccgccgc
     8461 aagctcgccg gcacgaccat cgcggaggcc gagctcggcc accgcaccgc cggtgtgtac
     8521 gacgacgacg attcgtcgga agcagcagcg ttcttcgatc aggaactcga ttgatcccag
     8581 cccgacggac gcgggaggtt ggaaattgac cggcggcaac ccacttctca tcaacgtcgt
     8641 cgagcttcgc cgctcggtcg gcaaccagaa gacggtgtcg gaatcggtcg agctcgacga
     8701 cgtcgggctg gcgagcgtgc gcgtcgaggt cggttcggcg ctacaggtcg acctcctgct
     8761 cgagacgttg tcgggcggca tcgctgcgac cggcaccgtc acgggccgtt ggaacggcga
     8821 gtgccgacga tgcctcgaga aggtggaggg tccgctcgac gtcgaggtcg ccgaggtgtt
     8881 cgaggagcac ccgaccgagg gcgagacgta tccgatctcc ggtgaccaca tcgatctgga
     8941 gcccatggtg cgcgagctgg ccatcctcgg gctgccgatc aacccgctct gccgcgacga
     9001 ctgtgccggg cccgtgcccg acgtgctgcc ggtgtcggtc gagagcgacg aagaagccga
     9061 accggcgccc gatccccgct gggcggcgct cgacgccctg cgcgacctga cctgacgggg
     9121 ctgccgcagc cggagttacc accgacgaac cggacgcggc tatgctctct cgttcgtgcg
     9181 ccacggtccg tgcgcgccgc caagaccacc tccccacgag tgagcaggaa cgacgatggc
     9241 cgtccccaag aagaagatgt cgaagtcgaa gacccgcatg cgtcgggcgt ctgcttggaa
     9301 gctctcggcc ccgtcccgca gcctgtgccc ccgttgcggt gtgtcgaagc ttccgcacgt
     9361 cgtgtgtggc aactgtggtt ggtaccacgg tcgccaggcc atcgaagtcg actgatcgac
     9421 gatcgcgggt gttggtcggc tgaccgaccg atgcgcacat aacggattta ggtcgtctaa
     9481 gctgccgtcc ggctcggccc tcgggggtgc tggtggcggt tgctccgcct cagttcggtc
     9541 gaaccgacga acgtttcttc gaggagttgc cgcgatgcct gccgagaccc atacccactt
     9601 cgcgtccctc gatcgtcaac ggatcgtcga gatcgtccag gaacgcctgg gtgagatcct
     9661 cgagatcgag ccgtcgacga tcaaggagac cgattcgctc gtcgacgacc tccacgccga
     9721 ctcgctcgcg cagtaccaac tggtggaggc gctggtcgac gagttcggcg agcgcaccgt
     9781 gggtcccggc atcgaagtcg aagacctcga agacctcgag ctcgtgagcg acgctgtcga
     9841 ctatgtcgtc gcccgactcg cttgagcctg gccgactcgt cctgctgggc gatctgtcgc
     9901 gccgtctcgg ccacgacttc tcgggtctct cgctgctcga cgcggcactt cggcaccgtt
     9961 cgtggtgcgg tgagcacggc gccgtcccat cgaacgaacg gctcgagttc ctgggcgacg
    10021 ccgtgctgca gctcgccatc accgaacgac tcttcgccgc cgacccaccg ctgcccgagg
    10081 gtgtcctcgc tcagcgtcgg gcggcgctcg tgaacacacg cacactggcc aatgcggcac
    10141 gccgcgtcga tctcggtccg gcgattcgcc tcgggcgcgg cgaggccgcg acgggcgggc
    10201 acgacaagga ctccatcctc gccgacagcg cggaggccgt gatcggtgcg gtgcatctcg
    10261 acgccggctt cgacgccgcc cgtgccgtca tcttctcgct cctcgaaccc gatctcgagc
    10321 gggtcgaggc gggcaaggac accggtgact cgaagtcgcg gctgcaggaa cgagcggctc
    10381 acgagctcga tctggtgccc cgctacgagg tctccggcgc cggtcccgac cacgcccggg
    10441 agttcgaggc agcggtcctg ctggacggcg tcgaatgggg tcgcggcgag ggccgaacga
    10501 agaaggaagc cgagcaggtg gccgcagctg cagccctgca gcggctggtc gacgaaggga
    10561 aactgaccga tgagcatggc tgaactgcct gagctggaga tcctccggcg cgacctggac
    10621 aaggacgtgg tgggcaagaa gatcaagacc gtcgaggcgc cgagcgcggc cacgatcaag
    10681 cgcgccggca ccaagaagtc gctgatcagc cggctcgagg gggcgaagat cgacagcgtg
    10741 agccgcaagg gcacgtggat cctgctcggt ctcgacactg gcgaagtcct catgatcggt
    10801 ctcggcgacc gcgcccaact tcagcgcaac cagaacaagg acgccgtggc caagggcacg
    10861 gccgtagtgc tgaccttcac ccagcacggc cagctccgcc tcgtcgaccc ggccaagagc
    10921 tccgagctgt tcgtcgtcac ggccgaagag gtcgacgaca tcgtggggcc gctcgggctc
    10981 gacctcgtcg acgaacccgt gtcctggacg aagttcggtg agatggtgct gcgccggggc
    11041 ggcaagctca agggcatcct gatggacccg acgatgctcg tcggcatcgg cccgatgtac
    11101 agcgacgaga tcctcttcga ggccggcctt cggtacgacc gtctggccga caagctgtcg
    11161 acgcaggaga tccggcgtct gtaccgggcg tcggtcgaga tcgttcacga cgccgtcaag
    11221 cacggtggtg cgacggtcgg cgccgacggg ttcctcaccc tgtcgggcga gcccggcggc
    11281 tacggcccca tgatcaacgt ctacggccgc gacggcgaga tgagcccgcg tgcccgcggc
    11341 cccatcgtga aagccaagtt cggctccggc ttcacctact actgcgaaca gacgcaggtc
    11401 tgaacgagcg ccttcggcgc cgttcagacc tgcctcctga tggcctcggc cgcctacgcc
    11461 tcaggttcgc tcgctgcgct cgcttcttcc ggctgcgctc ctcggcagca aatccatgca
    11521 aagagtgccg acatccgccg gaatctgcat ggatttcggg ttccgggccc atctgaccgc
    11581 atcgacggtc gtcgcctagg ctccgggccg tgttcctgaa gacgctgcag atcaaaggct
    11641 tcaagtcgtt cgccgatgtg gcgacgctcc agctggagcc cggcgtcacc gtcgtggtcg
    11701 gccccaacgg gtcgggcaag tcgaacgtgg tcgacgcgat cggttgggtg ctcggcgctc
    11761 aggcaccgtc ggcggtgcga tcccagaaga tggacgacgt catcttcgcc ggctccgcga
    11821 agcgtcccgc gctcggtcgg gccgaggtcg gcctcaccat cgacaactcg tcgggtgtcc
    11881 tgcccatcga gttcaacgag gtcacgatca cccgcacgct cttccggtcg ggcgacagcg
    11941 agtacgccat caacggcgtc aactgccgtc tcctcgacat ccaggagctg ctcagcgact
    12001 ccggtgtcgg ccgtcagcag cacgtgatca tcagccaggg ccagatcgac gccgtcctca
    12061 acgccaaggc cgaggagcgc cgcctgatca tcgaggaggc cgccggtgtc ctcaagtacc
    12121 gccgccgcaa ggagaagtcg gagcgccggc tcaacgcgac cgaggccaac ctgctgcgca
    12181 tccaggacct cctccgtgag gtccgtcgcc aactccgccc gctcgagcgt caagccgacg
    12241 cggcccgtcg ccacgacgcc gtcatcaccg agctggccgc cctcaagctc cacctcgtcg
    12301 gccgcgaact cgcacggctc cgcacccagc tcgccgagtc caccacccgg cagaacgagc
    12361 tgcgcaacgc cgatcgtgag ctgcgccggg tgctggcggg gctcgacgcc gaggtgctgg
    12421 ccaccgaggc acaactgtcg tcgatgggcg gcgacgactt cggcgacgcg ctcgttcgct
    12481 acgagtcgct ccgcgaacgc gcccgtggcc tgcagtcact gctcaccgaa cgccgtcgtg
    12541 gcatcgatcg cgatcgcaac gcgttcgtcg accaggccgt cgtggcatcg ctcgaggccg
    12601 agcacgccca gctgagcgcc gagttgcgcg aggtcgagtc cgagggcgtg ggcgctgcca
    12661 ccggcagcgg agccctcgcc cgtgccgagg ccgagctggc cgaagcccgt gctgcattcg
    12721 aggccgactg gggcgacggt gtggccgcgc cgccgagcga ggccgcggag atccgcggtg
    12781 aactcgccgc gctgcgggcg accatggaac ggaccgagag cgacaaccgc cgcctctcca
    12841 accagcgtga aacgttggtg accaaggtcg agcgtcttgc cgccgagttc gagcgcatcg
    12901 gtggcgagcg tgccgccgcg cacgacaacg tcgagtccct ggcgcgccag gcggccgagg
    12961 ccgaagccga gcgggctgcc gccgaggagg cgatcgtcga gatcgaggct cgcgccaccg
    13021 ccgctgcggc cgaacatcac accgtggctg cccgcgccga ggcactggcc ctcgcgctcg
    13081 atggtgcccg ccagcgcgcc ggcgccgctc acctcgccga gctcgacggt gtcgtcggca
    13141 cgttgctcga cgtcgtgcag gtcgaggctg gttgggaatc cgcgttcgag gcagcggccg
    13201 gcgaggcgtt ggcctccgtc gtcgtggccg acgccgaggt ggcgcggcgg gcgctcgagg
    13261 cacttgcggg cggcgagctg agcggtgccg tcctggccat ctcccacgcc gtggcccagg
    13321 cgccccgcgt tgccgtgccc gtcggcgtcg gccgtccggt tcgagatctc gtcaaggcca
    13381 cccgcgccga cgtcgagccg ctgctcgacg ctctgctcgc cggcgccgtt gtgatcgagg
    13441 gcgagtgggc cgaggccgtc gacgtggcgc tcgcccatcc cgacgccacg atcatcaccc
    13501 gcagcggtga ccggttcggc accaccgggt ggcgcatcgg ccagtcgagc gccggagcga
    13561 ccggtgccgc gctcgccgag gccgaggagc agctgggcag cgcccgcgtg gctgccgagg
    13621 acaccgcgct cgagctggca cgggccaagg agcggctctc gactgcccgc cgtgacgcca
    13681 acgacgccga gaaggccaac gaccagaacg ccaaccgggt ccgcaccctc gacgacgtgt
    13741 tgcgccgcgc cgaacgcgac cgcaccgagg ccgaggccga actcgtcacc gtcgacgggc
    13801 acctcaacga gctcgcccag cgcatcgagc gcgaggtcac ccgtcgcacc gacctcgagc
    13861 agcggctgcc cgcgctcgag gccgtcgagt cggagttgtc cgagcgggcc cgcacgatgg
    13921 cggaggcccg gcgcaacctc gaagccaagg ccggcgaggt cggtgcgctc cgcaccgacg
    13981 tcgaggtcaa ggtggccggt gtcgaggagc gccgtcgctt cctcaccgac cgcctcgccc
    14041 aggtcgacga gcgtctcagt cgcaaccagg ccgagcgcct cgctgccgag cagaagcggc
    14101 tcgagctcga tgcccgcgcc gaggtcaccg acaccctcat cacgttcgtc gccgaccgtc
    14161 tcggtgccgt ggagtccgtg ctcgaggtac tccgggaggc tcggcgccgt cagtccgaga
    14221 agacccgtgc ggtcagcgcc cagctcgaag cgctccgccg tcagcgttcc accacggaga
    14281 aggaactcga ggagcaccgc gagcgcatcc aacgctccga gatctccgac gccgagaccc
    14341 ggctgcggct cgagaccgcc accgaatcac ttcgcagcga gctcgattcc gagcccgacg
    14401 tcgcgatcgc agctgagtgt cccgagctcc ccgacgggat gtcgccccag gcccggctcc
    14461 gcgagctgga gcgcgaggtg cgcctcatgg gcccgatcaa cccgctggca ctccaggagt
    14521 tcgaggcgct gcaggagcgc caccagttcc tcgaagcgca gctggaggac attcgggcga
    14581 cgcgcaagga gctgtcgaag gtcatcaagg cagtcgacga ggagatcgtc aacgtcttcg
    14641 cgtcggcgta cgccgacgtc tcccagaact tccaggcgct gttcgacatg ttgttcccgg
    14701 gcggccaggg cggtctcacg ctcaccgatc cgtcgaacct cctcgagacc ggcatcgaga
    14761 tcgaggcacg gccgtcgggc aagaacgtcc gcaagctctc gctcctctcg ggcggtgagc
    14821 gctcgctcgt cgcgttggcg tacctgttcg ccgtgttccg gagccgcccg tcgcccttct
    14881 acgtgatgga cgaggtcgag gcggcgctcg acgacgtgaa cctgcaccgg ttcctgtcac
    14941 tcgtggccga gttccgctcg accgcccagc tgctcatcgt gagccaccag aagcgcacga
    15001 tggaggcggc tgacgtgctg tacggcgtca cgatggagcc cggtggctcg tcgcgggtga
    15061 tcagcgagaa ggtcggcgcc gccagcggcg gctcctgatc ccggcggccg gccagcgacc
    15121 tacttcttct tggtggtcgt cgtcttgtcc gacgacgacg tcttctcggt cgtcgtcgac
    15181 ttgctcgttg gcttcgcggt cgtggtcgtc gggttcgcgg cttcgtcggc ggcgagcagg
    15241 catcgggcga ggatggtcga cagctcgccg ctcaccttct cggagatgtt ggggttgggg
    15301 ccgccggctc ggtcgagttc cttgttgatg gccgcgttgt ccttgatctg gtcgtacgca
    15361 cactcggcaa cgggcttggt gatgccgctc gctgtcgtct gtttcacgaa ctcgtccttg
    15421 gggaaggcct cgtcgccacc gcatcccgag agcaggaacg ccgatgcgag cgcgacggcg
    15481 aggactcggg gcggggtgaa cggacgcatg cccgcatggt accggcggag gagcaccggg
    15541 ttggttaggg gaggcgctgg aaccagacga gggacatcgc accgctcagt gccagcgcga
    15601 tgagcgcgcc cgccaccaac ttccaggtga cttcctgctg ttcgatgtcg tagccgacgg
    15661 cgctgccgag gcggtcgtac acctgctcga gttcgccggc tgtggcggcc gcgaatgact
    15721 ctccgcctgt ctccttggcg acctcggcca gggccggctc atcgacgggc accggcacca
    15781 attgtgtgga gcccgagccg tcgtcgatct cgacgacgcc gttgggggtg ccgtacgcga
    15841 tggtgaacac gggaaccttg gccttgttgg cacgctcgat ggcggtcgga accgggaggc
    15901 tctggccctg gtccttgccg tcggagatga gcacgatcac ggcggggatc ttcgtgtcgc
    15961 cctcgctcgg caccgagttg gcgatcgcgg tcaacgacgt gtcgatggcc gctccgatgg
    16021 ccgtgccctg accgagttcg aggttcttga tggcccgcac ggcagtgtcg cgttgggtgg
    16081 tgggcggaac cagcaacttg gtcgagccgc tgaagtcgac cacgccgagc cgcaccttcg
    16141 gcggcatcga ctcgacgaac ttgaccgcgg cgaccttggc ggcatcgatg cgggtcgggc
    16201 tgacgtcggt ggcctgcatc gacagggagg tgtcgatggc gaggatgatc gtggcccgct
    16261 ccttcggcac ccgttcctgc gcctgcggct gggcgacgcc gacgaccatg gccgcgaggg
    16321 cgagcaggta caccgatgcg acgacgtgtc ggcgccacga cggccgcttc ggtgcaacct
    16381 tgtcgaggag gtccatgttg gagaaccgga cggcgtactt ggtccgccgg aactgcatgg
    16441 cgatgtaggc cgcggccatc gccgccacgg cgatgagcca ccagaggcgg ccgggggaga
    16501 tgaagctcca gttcatgggg tgttccgatc agcgggggac tgggtggtgg ctcgaactcg
    16561 agggcagaac gtgacgatgc ggcacggtcg atgcacgggg catctacctg tgggcgccgc
    16621 cgacgttggc ctggcgacgt ttggtcgacg cgacgtggcg tgcgaggtcg agaagccagt
    16681 cgcggtcggt acggagttgc agatgatggg ctccggagcc ggcgatcgcc agccggatgt
    16741 cggagcgctg ctcggccgca gcggccgcgt agcgctcacg gagcccccga ttgttggtgt
    16801 cgatctcgcg ggtgcggccc gactcggtgt cgatcagcgt cagcacgccc atcttcggca
    16861 ggtcgagctc gcgcgggtcg agcacctcga ccgcaaggac ttcgtggcgc acgctcagcg
    16921 ttgccagcgc atggcgccac gtctcggggt cctcgagcca gtccgagatc acgaccacga
    16981 gcgatcgccg tcgagacacc gacgccgccc gtttgagggt tgcggccagg tcggccgcgg
    17041 gagctgcgtc ggcgcggggt gcgccctgga tctggtggag gatcgagagc aggtgcttgc
    17101 ggccctggcg tgcgggcacg gtgacggccc cgtggctgcc cgagatgacg gcgccgatgc
    17161 gattgccgcc ccgcgaggtc aggaagccga cgcctgcggc cgcggccagg accaggtcgc
    17221 gtttctccca ggtgacggtg ccgaagtcga ggctggccga ccggtcggcg acgatccacg
    17281 tctcgagctc gcggtcggcg atcgactcgc ggatgtgggg tgtgctcatt ctcgccgtga
    17341 cgttccagtc gatgcggcgg acgtcgtcgc ccgcctggta ctcccgcgcc tcaccgggct
    17401 ccgaaccgtg cccggggacg agaccccggt ggtcgccgtg gaggatgccg tcgagacgtc
    17461 tcgtgacgtc gagctccaac cgccgcagca cgtcggcagc ccgacggtcg cccatgggcc
    17521 ccgcaccggg gccgacgacg tcggtcccac cggcaggatc gggggaggag cgcttcgcca
    17581 cgggtcagcc ccaggcctgg gagtcggtgc ccgagcggta gtaggcgtcg ttcgggccgg
    17641 cgatctggct cggctggatg acgggagcgg ggaccgtgga cagcacgcgg atcatgatct
    17701 gctcggcgtc gacatcggcc gccaacgcct cgtaggtgag gacgaggcgg tggcgcatga
    17761 tctcgggcgc cacgtcgaag atgtcctggg ggaccacgta gttgcggccg cgcaggaggg
    17821 ccattgcccg gcccgcccgc accaggccga gactggcgcg agggctggcg ccgaactcga
    17881 ggaacggggc gaggtcggcg aggccgtagt tctgcggggt ccgggtggcc aacacgaggt
    17941 tgaccgcgta ctcgacgacg ctgcggtcgc agtaacacgc gtcggcggcg gcctggagcc
    18001 gcagcacgtc ggccaggctg accacctcgg ccggctgggg tggggagacg cccatgcggt
    18061 tcacgatctc cagctcttcg tgcggggtcg ggtagccgag
//
LOCUS       DAOSOA010000018        17995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3142_length_17995_cov_6.989409, whole genome shotgun sequence.
ACCESSION   DAOSOA010000018 DAOSOA010000000
VERSION     DAOSOA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17995
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_17995_cov_6.989409"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PKY13_02580"
     CDS             <1..503
                     /locus_tag="PKY13_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HOT78840.1"
                     /translation="GRVDRRPTGSRGLPNEPCDSWAEFYATQRLLPLARIAHDEQALP
                     DSLIAGLEGVARRLDELDGPPEPPARLHGDLWAGNRIIGPDGRSWLIDPAAHGGHREF
                     DLAMMQLFGGFGSETFAAYRAAYPLADGWAERVPLHQIAPLVVHAIKFGGAYRSSAAR
                     AIGQYQ"
     gene            504..1556
                     /locus_tag="PKY13_02585"
     CDS             504..1556
                     /locus_tag="PKY13_02585"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOT78841.1"
                     /translation="MVSPSDVRRIAINTGGGDAPGLNAVIRAVTLAGLDRDWEVLGIR
                     DGYDGLFSTGGVITLDRDAVRDITHLGGTMLGTTNRGNPFATGRGQEVADRLRDLQVD
                     VMVAVGGDGSLSIAHLLSQLGVPVIGVPKTIDHDLEHTDQTTGFDSAVSFATECIDRL
                     VSTTTAHGRIMVIEVMGRTTGWIALHSGIAAAANVILVPEIPYDIDIVAKRVRERAAR
                     GQEYSIIVVAEGASAVGGETTTVEGSTRLGGVAATIATQLESLTGKEARSLSLGHLLR
                     GGSPTATDRLLGLRYGAEAVIAIAAGARNVMVALDGDEAKLVPLADIAGRARLVPDDS
                     GALRTARSIGICLGDR"
     gene            1565..2314
                     /locus_tag="PKY13_02590"
     CDS             1565..2314
                     /locus_tag="PKY13_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOT78842.1"
                     /translation="MTEPTAVAFHFDVMCPFAYQTSIWIREVRRLTGLEIDWRFFSLE
                     EVNRVEGKKHPWERDWSYGWSMMRIGALLRRTDMALVDEWYLRAATALHVDGHKPHDP
                     AVARHLLETMGLDPTIVDRAIADPTTHDDVRSDHDAVIAAGGYGVPTLCFERPADQGI
                     STDADGPHQDWLFGPVVLDPPTGDAALRLWSAVTAWLEFPHLYELQRPKTVADGHYIA
                     ETFRPYLDARDWVSIDRGEQIDFGSYDPKSG"
     gene            complement(2321..2884)
                     /locus_tag="PKY13_02595"
     CDS             complement(2321..2884)
                     /locus_tag="PKY13_02595"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT78843.1"
                     /translation="MSRRKKQKGSPSGDAVSVVIAAPADRLYDIVTDVRRMGRLSPEC
                     TGGKWLGSATGPAVGARFRGHNRRGPIRWSTTNLVVSANRGREFAFETKQSGARWRYR
                     FEPSEVGGTTVTESHEMFRRRPAVAWVFANLLLGGASSHDEEVKAGMRATLERLREVA
                     EAPEVSARRRRRGRRARRAERRAALAA"
     gene            complement(2937..5102)
                     /locus_tag="PKY13_02600"
     CDS             complement(2937..5102)
                     /locus_tag="PKY13_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78844.1"
                     /translation="MGRGLVATYSLTAGLGSALLFLVEPMMARQLLPLLGGSAAVWNT
                     AMVFFQCALLAGYLVAHLAQRGTGVGGRGRRVVVVAVIAAGVVALPLTLPDWRPPTGS
                     GQTWWVIGILAMSVGLPFVGLASLSPTLQRWFADTDHPHAADPYFLYAAGNVGSVIGL
                     LAYPIVVEPTLGLADQRRWWSAGYLLALALCVTCVVLRRRHLARPTADFAAESATARA
                     ETPPSRVARLRWIALAAVPSGLLLGVTRHLSTDVAAVPLLWVLPLLAYLGTFVVAFRR
                     GTAPSAKLVRGALLLSIPLIATMVSGVGSALVAVAIGIGGFTLLAMVCHSCLAADRPD
                     ATWLTSYYLWISFGGAVGGISVALVAPVVFTRVTEYPLLVVAALAVVMLLAPRSPTAP
                     GEPSRGPAVLAGVVVLAVGVLCVGLSRRGPEHAALMAQVLAVGGAAAYVIAKTPRRIA
                     AALAALVVVTVVVDGRSGLAESRTFFGVYRVSVDDDGRHVFEHGTTIHGEQEFAAGPT
                     TEPLTYYTPDSGVGRTFAALADDPAALDVAVVGLGAGTLAAYGRPDDHIDFYEIDGEV
                     VRIARDPRLFTFLRDTRATVSTVVGDGRLELARSERPLDLLVLDAFSSDAIPVHLLTA
                     EAMDEYRQHLVPGGVIAVHISNRFFDLAPIVSRLGERAGLQAHRLSTSVSTWMLLSDD
                     RAPAALQDELADPAWRLVEPDGSAPLWTDDFSNLLSALE"
     gene            complement(5248..6246)
                     /gene="pstA"
                     /locus_tag="PKY13_02605"
     CDS             complement(5248..6246)
                     /gene="pstA"
                     /locus_tag="PKY13_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOT78845.1"
                     /translation="MAATATIDTAPDPAGESPLQHPLESSLSTGRSWRNRLASAWMIA
                     SLIVAAIPLLAVLGILVVKGFSLVASLDWWSKPIPSNVASNALGGQEAQEAFGNVGTA
                     APVKEVIYGMQPAIVGTLITTGLAALLSIPLGIAAAVYLNEYGGKSRSATIIRFFTDV
                     MTGVPSVVMGIFIYTVWVLNFEASGRSGLAGGLALACLMLPVVVRSTEEMLRLVPDSL
                     RQASAALGTPRWKTTTKVVLPAAMPGITSGAMLAIARAAGETAPILFTVGVVSSTNFS
                     LFGQNTTLSAQIFSNATQPGGESIAWGAALTLIVIVMAFTLTARFVSSKFSVRVDG"
     gene            complement(6246..7223)
                     /gene="pstC"
                     /locus_tag="PKY13_02610"
     CDS             complement(6246..7223)
                     /gene="pstC"
                     /locus_tag="PKY13_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOT78846.1"
                     /translation="MTDLSLRPITVDDLAGDRNGARPDTLFRAGAKGAGYLVLVILAA
                     IAVSTTVKAWPAFRAEGFGYFFRDDWVPSQGRFGARSIIYGTLVVSAIGLVVAVPLSL
                     GIALFITELAPRRLRSSITTVMDVLASVPSVVFGLWGFLILRPHLKDFYNSVADAVSG
                     IPVLRSIFGPSLSGQSFMTAGVIVGLMIVPIITSIVREVFQTVPINEKNGALALGATR
                     WEMIQGVVFPHSTGGIVGAVMLGLGRAMGETIAVALVIGASPQITANLFGQGEAMPSV
                     IARNLNEASGTYQSALIGLGVGLFVLTILINISARVLVRRVDAATRGAS"
     gene            complement(7232..8377)
                     /gene="pstS"
                     /locus_tag="PKY13_02615"
     CDS             complement(7232..8377)
                     /gene="pstS"
                     /locus_tag="PKY13_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOT78847.1"
                     /translation="MKNSRRYRPGRIAVLPLALLAILGLVVTACGDDTETADDSGSSE
                     TTAAASEATTTTVAGLDIDYASLEGTLNGSGSSFQDALEQTVKGEFEKVATGVKVNYA
                     KSGSSAGKADLAGNTVQFAGTDSLIKDADKATFTGGEVLYFPIAAAPITVSYNLAGVE
                     ELNLSADVIAAIFQGEITSWDDAKIAADNPDAKLPATPIAVVHRSDGSGTTSNFTKYL
                     KKAAPSVWKLDAGDTVNWPATTQGAEKNSGVAAIISSTDGAVGYVDLADSVSAELTRA
                     NVKNSAGNFVEPKLPGASAALAGAEIADDLTYDPLDADGDDAYPITSPTWVIVYKTQT
                     DPKVADALKGYLNFMLTEGQTLAPTVGYAPLPAELQAKAIAQLDMIG"
     gene            8556..8834
                     /locus_tag="PKY13_02620"
     CDS             8556..8834
                     /locus_tag="PKY13_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78848.1"
                     /translation="MRVRIRPKADPLAVLVALLAAPLGAASCSDPGDVSVSRSDDPRS
                     GGKSGFDVTTTERLGGTVPADDVEAQLTAPPLRLRWRAGSARCRTRRA"
     gene            8831..8971
                     /locus_tag="PKY13_02625"
     CDS             8831..8971
                     /locus_tag="PKY13_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78849.1"
                     /translation="MTHLIAQRNLPFSGEVAVRVYVDGGDRSNGGYVQYLANGTLDRV
                     QA"
     gene            9017..9490
                     /locus_tag="PKY13_02630"
     CDS             9017..9490
                     /locus_tag="PKY13_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT78850.1"
                     /translation="MLDQQEISDRLELVDLMVRYSHAVDTRSFGDFDHIFTEDAVIDY
                     TAFGGPRGTVDEIKAYLTETLAPFPTFQHLVANPLLVIDGDVASGRTMCFNPMGVARP
                     EGESGEPRMFFCGLWYEDQFVRTGDGWRIRERFEVKSWTHNMGPAFSGGAAARKD"
     gene            9495..11033
                     /locus_tag="PKY13_02635"
     CDS             9495..11033
                     /locus_tag="PKY13_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT78851.1"
                     /translation="MATIWQLIERRAGETPDDVMLIDESDRRVTFGEFRDQAAALAAG
                     LAGAGIGSESRVTWQLPSSIDAAITMAALSRLDAVQCPVLHLYRKKELGFVLKHTGAE
                     YCIVPGEWKGFDYTAMADELAADLDPSPVVLTLGSLPTGDPSTLPPAANPADGDEPVR
                     WIYSTSGTTSDPKGVQHTDATLIAGGQGLADALQMTSADVGSIAFPIAHIAGPDYIIM
                     MLIAGMSAVIIEAFVPDQAIATYKKNGVTMAGGATAFYQMFLAEQRKQPGDPIIPTLR
                     ALSGGGAPLPPELYHEVVAEMGIKVCHGYGMTEIPMITQGSPTDNDDQLAHTTGTPVT
                     GADVRIVAEDGSVAPAGVDGEVRVKGPMVTKGYTNPALTAEAFDDDGYFRTGDVGHLR
                     PDGHVVLTGRLKDIIIRKGENIAAKEIEDLLYAHPKVGDVAVIGLPDRERGERVCAVV
                     EPPAGGEVITFDEMVAYLGEQGLTRFKTPEQLEIVDALPRNETLRKVLKYQLRDTFAA
                     VPWP"
     gene            complement(11035..11439)
                     /locus_tag="PKY13_02640"
     CDS             complement(11035..11439)
                     /locus_tag="PKY13_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78852.1"
                     /translation="MLRVVEHRGLRDRLLALVDDLEHDADWDPVQEIRATHELADLAA
                     ELDAARREQAALLASIAAVETDLGRIRRERDDARRRLAELREQVRPELPDELVQLRVR
                     LEADRPTEPPLLALAQAERLARAVRSTLVEDS"
     gene            complement(11692..14118)
                     /gene="lon"
                     /locus_tag="PKY13_02645"
     CDS             complement(11692..14118)
                     /gene="lon"
                     /locus_tag="PKY13_02645"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT78853.1"
                     /translation="MSQATPSESDRSDLDHTDLSHTLPLLPLTSGVVLPGMVITMALE
                     TDEARAAAEAATTADGRLLLVPRVDGRYANVGTVSVIEESGKLPGGTLGLVVRGERRA
                     RIGAGVPGTGAALWVQVEPVDPEPVSDAALELAKEYRAVIENILETRGAARVIAMLPP
                     VTDADALADSAGYSPDLDFEQKVEVLETTDVEARLRLVLGWAKETLGDLSIRQRIRSD
                     VEEGMEKTQREFLLRRQMDAIKKELGQLDGSEASPTDDYRAQIADRDLPDKVRSAVEK
                     EIDKLERTSEQSPEHGWIRTWLDTVLELPWGETSEDRLDIAEAARILDADHDGLEDVK
                     ERILEHLAVRKLRAERKLAPVEGRGAGAILTLVGPPGVGKTSLGESVARALGREFVRV
                     ALGGVRDEAEIRGHRRTYVGAQPGRLVRAISDAGTMNPVVMLDEVDKLGGDYRGDPSS
                     ALLEVLDPAQNHTFRDHYLEVELDLSDVLFVATANVMDTIPGPLRDRMEIIGLDGYTE
                     EEKIAIARHHLVDRQLDRTGLRPDEVTLTDEALRTIVADYTREAGVRSLEREVGRLLR
                     KVATKLASGEQTAPVTVDAADVAGWLGRARFFFEAADRTSAPGVATGLAVTGVGGDVL
                     FVEATSMAGPEGLTITGQLGDVMKESAEIALSYVRSHAAELGIDPMTFEKRRFHLHVP
                     AGAVPKDGPSAGVTMVTALVSLLTDRPVRPVVGMTGEVTLQGKVLPIGGLKQKVLAAH
                     RAGLTEVVLPKRNAGDLEDVPEAVRDQMTFHIAADIGEVLAAALRPAGATDEADEWDA
                     AHPPTELLAV"
     gene            complement(14260..15852)
                     /locus_tag="PKY13_02650"
     CDS             complement(14260..15852)
                     /locus_tag="PKY13_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOT78854.1"
                     /translation="MQQAEIDAFQFVGQDIPWLLNHWAAAAPDRPFLVWEPRSGEDRT
                     WTYRQFVDDVEKVASALVARGVKLGDKVLIHAENCPEAVLAWYGCARIGAVGVTTNTR
                     SVAAEINYFATHTGCVGAITQPQFAAVVNEAVPDLGWFVVTADDSGIAATAEPVDRPH
                     DGFDQLLEHDLPEAPRHTPDPLLPAGILFTSGTTSRPKAVVHTHGNALWAAKVGPQNI
                     DMDEGDVYLIYLPFFHVNAQSWSIWTSLGAGGTVILQPKFSASKFWEVILKHKVTHIS
                     LIPFVFKAVAPQPIPDHTVKVGVFGLIMPELVDWLKMRVVAAYGMTETVIHAVRDNKY
                     ERYPRLAMGRVAPGYEMIVVDPETKEIVKEGEIGEIWLRGTRGIQLFLEYYDNEEANN
                     KAFNADGWLQTGDLVLLGPDGNLEYRDRDKDALKVGGENVSAREVEDQVRTVPGIADV
                     AVVGKSHEMLDQVAVAFVVKADPNQDDDELAAAIIAACKTNLADFKVPRAVYFVDDFP
                     KATLDKVAKNELREMADAHPAV"
     gene            16336..17013
                     /locus_tag="PKY13_02655"
     CDS             16336..17013
                     /locus_tag="PKY13_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78855.1"
                     /translation="MTFGRGESCEARFGHAPVRDWEIPRVAGWLVIAHGRIFVEAAGM
                     PDPHRGEAGRPQPVRRALQVSAAGGPPVPVAAGSAYSPSEEIFTIEVVGATGTWELDV
                     VARSRDEGEDPTVHEPHTHSVVIDLTDVQREVLLAYAEPVLAGSVEPATHDQVAAKVY
                     MSRSQVRRHLERLSDEFFAKRLWSPDSGDTRVRVVETARHNHILTESARRHSDATSPA
                     ADRPAGH"
     gene            17061..>17995
                     /locus_tag="PKY13_02660"
     CDS             17061..>17995
                     /locus_tag="PKY13_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOT78856.1"
                     /translation="MPLVDGRPVPEPGGGGDGLVYRATDHEGNDVALKLLLHARAADL
                     PDAAVRLERIAVLAEPHLMRPIEVFVGTALTNDPEPDIEEADVCWMAAEWVDGRRLEH
                     VATDDDPEQNLRHVAQIARGVAALHRAGGIVHRDVKSGNVRVTPDGRAVLIDYGSPGR
                     PSSDVPAGTPGWLSPEVAAGHAPGHAADVWGVGAIAHHLLVGSPPRLDGAACSRERLQ
                     FMGARTGLRDADELATHISRLLESEPGRRPDDLDRWADRLDAVLDGRGRSHRRRLVAA
                     TGVVAIGALALIAVVGGNDDPETPTAAASASASAS"
BASE COUNT         2883 a         6259 c         6237 g         2616 t
ORIGIN      
        1 tcgggcgtgt ggaccgtcgg cccacgggca gccgcgggct ccccaacgaa ccgtgcgatt
       61 cgtgggccga gttctacgcg acgcagcggc tcctgccact ggcgcggatc gcccacgacg
      121 aacaggcgct ccccgacagc ctgatcgccg gcctcgaagg ggtcgcgcga cgactcgacg
      181 agctcgacgg cccacccgaa ccgccggccc ggctccacgg cgacctctgg gcgggcaatc
      241 ggatcatcgg tcccgacggc cggagttggt tgatcgatcc ggccgcgcac ggcggccatc
      301 gcgagttcga cctcgcgatg atgcagctgt tcggaggctt cggttcggag acgttcgcgg
      361 cgtaccgcgc ggcgtacccg ctcgccgacg gatgggccga gcgggtcccg ctgcaccaga
      421 tcgcaccgct cgtcgttcac gccatcaagt tcgggggtgc ctatcggtcc tccgcagccc
      481 gcgccatcgg ccaatatcag tagatggtct ccccctccga cgtccgccgc atcgccatca
      541 acaccggggg cggcgatgct cccggtctca acgccgtcat cagggccgtc acgcttgccg
      601 ggctcgaccg cgactgggag gtcctcggta tccgcgacgg ctacgacggg ctgttctcca
      661 ccggcggggt catcaccctc gaccgcgacg cagtgcggga catcacccac ctcggcggca
      721 cgatgctcgg gacgaccaac cggggcaacc cgttcgccac cggtcgcggc caggaggtcg
      781 ccgaccgact ccgtgacctg caggtcgacg tcatggttgc ggtcggcggc gacggttcgc
      841 tgtccatcgc ccacctgctg agccagctcg gcgttcccgt catcggcgtc ccgaagacca
      901 tcgaccacga cctcgagcac accgaccaga cgacggggtt cgactccgcc gtctcgttcg
      961 ccaccgagtg catcgaccgt ctggtcagca cgaccaccgc ccacggccgc atcatggtga
     1021 tcgaggtgat gggccggacc accgggtgga tcgcgctcca cagcgggatc gccgctgctg
     1081 cgaacgtcat cctcgtcccc gagatcccct acgacatcga catcgtcgcc aagcgggtcc
     1141 gcgaacgcgc ggcgcggggc caggagtact cgatcatcgt ggtcgccgag ggcgccagcg
     1201 ccgtcggggg cgagaccacc accgtcgagg ggtcgacccg gctcggcggt gtcgccgcga
     1261 cgatcgccac acaactcgag tccctcaccg gtaaggaagc acgctcgctg tcgctgggcc
     1321 acctgctccg gggtggctcc cccaccgcca ccgaccgcct cttgggcctg cgctacgggg
     1381 ccgaggcggt catcgcgatc gccgctgggg cccgcaacgt catggtggcg ctcgacggcg
     1441 atgaggccaa gctcgtgccg ctcgccgaca tcgccggccg cgccagactc gtgcccgacg
     1501 acagcggtgc gctccgcacc gcccgctcca tcggcatctg cctcggcgac cggtaggcga
     1561 cacgatgacc gagcccaccg ccgttgcctt ccacttcgac gtgatgtgcc cgttcgcgta
     1621 ccagacatcg atctggatcc gcgaggtacg tcggctgacc ggtctcgaga tcgactggcg
     1681 gttcttcagc ctcgaggagg tcaaccgcgt cgagggcaag aagcacccat gggaacgcga
     1741 ctggtcctac ggatggtcga tgatgcgcat cggcgcgctg ctgcgccgga ccgacatggc
     1801 gctcgtcgac gagtggtacc tccgcgccgc cacggcgttg cacgtcgacg gccacaagcc
     1861 ccacgacccc gccgtcgccc gacacctcct cgagacgatg gggctcgacc ccacgatcgt
     1921 ggaccgggca atcgccgatc cgaccaccca cgacgacgtt cggtccgacc acgacgccgt
     1981 gatcgccgcc ggcggctacg gggtgccgac gttgtgcttc gaacggccgg ccgaccaggg
     2041 gatctcgacc gacgccgacg ggccgcacca ggactggctg ttcggaccgg tggtgctcga
     2101 cccgcccacg ggcgatgccg cgctccggtt gtggagcgcg gtcaccgcgt ggctcgagtt
     2161 cccccacctg tacgagctgc agcgaccgaa gaccgtcgcc gacggccact acatcgccga
     2221 gacgttccgg ccgtacctcg atgcccggga ctgggtcagc atcgaccggg gcgaacagat
     2281 cgacttcggg tcctacgacc cgaagtcggg ctgaaccggc tcaggccgcg agcgcggcgc
     2341 ggcgttccgc ccggcgggca cgtcggcccc gacgacgccg acgagccgac acctcgggcg
     2401 cctcggcgac ctcccgcaga cgctcgagcg tggcgcgcat gcccgccttg acctcctcgt
     2461 cgtgcgacga cgcaccgccg aggagcagat tggcgaacac ccacgcaacg gcggggcgac
     2521 gacggaacat ctcgtgggac tcggtcaccg tggtgccacc gacctccgac ggctcgaagc
     2581 ggtaccgcca ccgcgctccg ctctgcttcg tctcgaaggc gaactcccgg ccacgattcg
     2641 ccgagaccac gaggttggtc gtcgaccaac ggatcggacc ccgccggttg tggccccgga
     2701 accgggcacc gaccgccgga ccggtggccg acccgagcca cttgccaccg gtgcactcag
     2761 ggctcagccg acccatacga cggacgtcgg tgacgatgtc gtagaggcga tcggcggggg
     2821 cggcgatgac cacggacacg gcgtcgcccg acggcgaccc cttctgcttc ttgcgacggc
     2881 tcatggacag aacgtagcca acctcgtccc gttccgggcg gctgtccgac gtcgtcctac
     2941 tccagcgccg agagcaggtt cgagaagtcg tcggtccaca gcggcgccga cccgtcgggc
     3001 tcgacgagtc gccacgccgg gtctgccagc tcgtcctgca acgctgcggg cgcacgatcg
     3061 tcggacaaca gcatccacgt cgacaccgac gtgctgaggc ggtgcgcctg cagaccggcc
     3121 cgctcaccga gacggctcac gatcggtgcg aggtcgaaga accggttcga gatgtgcaca
     3181 gcgatcaccc cgccggggac gaggtgctgg cgatactcgt ccatggcctc ggcggtcagg
     3241 aggtgtacgg ggatggcgtc ggagctgaac gcgtcgagca ccaggagatc aaggggccgc
     3301 tcggaccggg ccagttcgag gcgtccgtcg ccgaccaccg tcgagaccgt ggcccgggtg
     3361 tcgcggagga acgtgaacaa tcgagggtcg cgagcgatgc gcaccacctc cccgtcgatc
     3421 tcgtagaagt cgatgtggtc gtcgggccgc ccgtacgccg ccaacgtgcc ggccccgagg
     3481 cccaccaccg cgacatcgag cgctgcggga tcgtcggcga gcgccgcgaa ggtccgcccc
     3541 accccggaat ccggcgtgta gtaggtcagt ggctccgtcg tcggaccggc cgcgaactcc
     3601 tgctctccgt ggatcgtcgt gccgtgctcg aacacgtgtc gtccgtcgtc gtcgacgctc
     3661 acccggtaca cgccgaagaa cgtgcgtgat tccgcgagac cggaccgacc gtcgacgacg
     3721 acggtcacga cgacgagcgc ggcgagcgca gcggcgatgc gacgtggggt cttcgcgatc
     3781 acgtaggcag cggcaccgcc taccgcgagc acctgcgcca tcagggcggc gtgttcgggt
     3841 ccacggcggg agaggccgac gcagagcacc ccgacggcga gaacgacgac gcctgcgagc
     3901 acggccgggc cccgggacgg ctcgcccgga gccgtcgggg atcggggagc aagcaacatc
     3961 acgacggcca gagccgccac gaccaggagg gggtactccg tgacccgcgt gaacaccacg
     4021 ggtgcgacga gtgcgaccga gatgccaccg acagcgccgc cgaacgagat ccacaggtag
     4081 tacgaggtga gccacgtggc gtcgggtcga tccgctgcga gacacgagtg gcagaccatc
     4141 gcgagcaggg tgaacccacc gatgccgatg gcgacagcga cgagcgccga cccgacgccg
     4201 ctgaccatcg tcgcgatcag cgggatgctc agcagcagcg ctccccgcac gagcttcgcc
     4261 gagggggcgg tgccacggcg gaacgccacc acgaaggtgc cgaggtaggc caacagcggg
     4321 agcacccaga gcagtggaac cgcggcaacg tcggtcgaca ggtgtcgggt gacgccgagc
     4381 agcagacccg acggcacggc agcgagagcg atccagcgga gacgggcgac acgactgggc
     4441 ggggtctccg ctcgcgcggt cgccgactcg gccgcgaaat cggcggtcgg gcgtgccagg
     4501 tgccggcgcc ggagcaccac acaggtgacg cacaaggcga gggccaacag gtacccggcg
     4561 ctccaccatc gccgctggtc cgccagtccg agggtcggct ccacgacgat cggataggcc
     4621 aacaggccga tcacgctgcc gacgttgccg gccgcataca ggaagtacgg gtccgccgcg
     4681 tgcgggtggt cggtgtcggc gaaccatcgc tgcagggtcg ggctgaggct cgcgagaccg
     4741 acgaacggca ggccgaccga catggccagg atgccgatga cccaccacgt ctgcccgctc
     4801 cccgtcggcg gtcgccagtc gggcagcgtc aacggcaagg ccaccacacc tgcggcgatc
     4861 acggcgacga ccacgacgcg tcggccacgt ccgccgacac ccgtcccgcg ctgggcgagg
     4921 tgcgccacca ggtacccggc gagcagggcg cactggaaga acaccatggc ggtgttccag
     4981 acggcggcag acccgccgag gaggggcagg agttggcggg ccatcatcgg ctcgaccagg
     5041 aacagcaggg ccgacccgag ccccgccgtc aggctgtacg tcgcgaccag accgcggccc
     5101 accgcgcgct cctccgcccc gggacgcgtg gcaggcagag tggcggtcac cccacctaca
     5161 tcggcaacgt cccggagttc tgaagcgcga ggagcgactc cgtcggtgct cctcgcagcg
     5221 ggaaccccgg cggtcgagga gccggggtca gccgtcgacg cggacggaga acttcgagga
     5281 gacgaagcgg gcggtcagcg tgaacgccat cacgatcacg atcagggtga gggcggcccc
     5341 ccaggcgatc gactcaccgc cgggctgtgt cgcgttcgag aagatctgcg ccgacaacgt
     5401 ggtgttctgc ccgaagagcg agaagttggt cgaggacacg acgcccaccg tgaacaggat
     5461 gggcgcggtc tcgccggcgg cacgggcgat ggcgagcatc gctcccgacg tgatgccggg
     5521 catcgctgcg gggagcacga ccttggtcgt cgtcttccaa cgcggcgttc cgagcgcagc
     5581 cgacgcctgg cgcagcgagt cggggacgag ccgcagcatt tcctcggtcg atcgaacgac
     5641 gacgggcaac atcaggcacg cgagggccaa gccgccggca agccccgacc gcccactcgc
     5701 ctcgaagttc agcacccaga ccgtgtagat gaagatgccc atcaccaccg acggcacacc
     5761 cgtcatcaca tcggtgaaga agcggatgat cgtcgccgat cgactcttgc cgccgtactc
     5821 gttgaggtag acggcagcgg cgatgccgag cgggatcgac aagagggcgg cgaggccagt
     5881 ggtgatcaac gtgcccacga tcgcaggctg catgccgtag atgacttctt tgaccggcgc
     5941 agcggtgccg acgttgccga acgcttcctg cgcctcctgg ccaccgagcg cgttcgacgc
     6001 gacgttcgaa gggatcggct tgctccacca gtcgaggctg gccacgagcg agaagccctt
     6061 cacgaccagg atgccgagca cggcgaggag cgggatggcg gcgacgatga gcgacgcgat
     6121 catccaggca ctggcgaggc ggtttcgcca actgcgaccc gtcgacaggc tgctctcgag
     6181 cgggtgctgc agcggcgact cgccggcggg gtcgggtgcg gtgtcgatgg tggcggtcgc
     6241 ggccatcagc tggctcccct ggtagcggca tcgacgcgtc gcacgaggac ccgcgccgaa
     6301 atgttgatga ggatcgtgag cacgaacagc ccgacgccga ggccgatgag cgccgactgg
     6361 tacgtgccgc tcgcctcgtt gaggttgcgg gcgatgaccg acggcatcgc ctcgccctgg
     6421 ccgaagaggt tggccgtgat ctgcgggctg gcaccgatga ccagcgccac ggcgatcgtc
     6481 tcgcccatcg cacggcccag gcccagcatg accgcgccga cgatgccacc ggtcgagtgc
     6541 gggaacacga ccccctggat catctcccac cgggtggcac ccagcgcgag tgcgccgttc
     6601 ttctcgttga tcgggacggt ctggaacacc tcccgcacga tcgacgtgat gatcggcacg
     6661 atcatcagac cgacgatcac accggcggtc atgaagctct ggcccgagag gctcggtccg
     6721 aagatgctgc ggagtaccgg gatgccgctg accgcgtcgg cgacgctgtt gtagaagtcc
     6781 ttgaggtggg ggcgaaggat caggaacccc cacaggccga acaccaccga cggcaccgac
     6841 gccagcacgt ccatcaccgt ggtgatggac gagcggaggc gtcgcggtgc cagttcggtg
     6901 atgaacagcg cgatgccgag gctcagcggt acggcgacca cgagcccgat ggccgacacg
     6961 acgagcgtgc cgtagatgat cgaccgggcc ccgaaacgtc cctgactcgg gacccagtcg
     7021 tcacggaaga agtacccgaa cccttcggcg cggaacgccg gccaggcctt gacggtcgtg
     7081 ctcaccgcga tggcggcgag gatcacgagc accaggtagc cggcgccctt ggcgcccgcc
     7141 cggaacaagg tgtccgggcg ggcgccgttg cgatcaccgg cgaggtcgtc gacggtgatg
     7201 ggacgaaggg acaggtcggt catgtcgtcg actaaccgat catgtcgagc tgggcgatcg
     7261 ccttcgcctg cagctcggcg ggaagcggtg cgtagccaac ggtcggggcc agggtctggc
     7321 cctcggtcag catgaagttg aggtagccct tgagcgcatc ggcgaccttg ggatcggtct
     7381 gcgtcttgta gacgatgacc caggtcggcg aggtgatcgg gtaggcgtca tcgccgtcgg
     7441 cgtcgagcgg gtcgtacgtg aggtcgtcgg cgatctccgc accggcgagc gctgcggacg
     7501 ccccggggag cttgggctcc acgaagttgc cggccgagtt cttcacgttg gcacgggtca
     7561 gctcagccga caccgagtcg gcgaggtcga cgtagccgac ggcgccgtcg gtcgacgaga
     7621 tgatggcggc cacgccgctg ttcttctcgg cgccctgggt ggtggcgggc cagttgacgg
     7681 tgtcaccggc gtcgagcttc cacaccgacg gggccgcctt cttcaggtac ttcgtgaagt
     7741 tggaggtggt gcccgaacca tcggagcggt gcaccacggc gatcggcgtc gccgggagct
     7801 tggcgtcggg gttgtcggcg gcgatcttcg cgtcgtccca cgaggtgatc tcaccctgga
     7861 agatggcggc gatgacgtcg gcgctcaggt tgagctcctc gacgcccgcc aggttgtacg
     7921 acacggtgat cggcgcggcg gcgatcggga agtacaggac ctcaccgccg gtgaaggtcg
     7981 ccttgtcggc gtccttgatc aggctgtcgg tgccggcgaa ctgcacggtg ttgccggcga
     8041 gatcggcctt accggcggag gagccggact tggcgtagtt gaccttcacg ccggtggcga
     8101 ccttctcgaa ctcgcccttc accgtctgct cgagcgcgtc ctggaaactc gagcccgagc
     8161 cgttgagggt gccttcgagg gaggcgtagt cgatgtcgag tccggcgacc gtggtggtcg
     8221 tcgcctcgga cgctgcggcc gtcgtctccg acgaaccgga gtcgtcggcg gtctcggtgt
     8281 cgtcgccgca ggcggtgacg acgaggccca ggatcgccaa caaggcgagc gggaggacgg
     8341 cgatccggcc gggacggtat ctacgggagt tcttcaaggt gtcagcctcc ttggctggtg
     8401 aagcggcatc cggtcacggc tgccgcctgg caggggtgtc tctgtcagga gcgaacctag
     8461 gaatcgctgg ttgacaccca gcggtgacca ggtgaacgca agatgaacga ctcggaaaga
     8521 ttcgacgccg acccccgccg ccctccgctc gtgcaatgcg cgttcggatc cgaccgaagg
     8581 ccgaccccct ggccgtgctc gtcgcgctgc tcgctgcccc gctgggcgcc gccagctgct
     8641 ccgaccccgg tgacgtgtcc gtgtcgagat ccgacgatcc ccgttccggc ggcaagagcg
     8701 gcttcgacgt gacgacgacc gaacggctcg gcggaaccgt ccccgccgac gacgtcgagg
     8761 cacaactgac ggccccaccg cttcggctgc ggtggagggc aggatcggcc cgctgtcgaa
     8821 ctcgacgggc gtgacccacc tgatcgcgca gcgcaacctg ccgttctccg gcgaggtcgc
     8881 ggtgcgggtc tacgtcgacg gcggcgaccg gtccaacggc ggctacgtcc agtacctggc
     8941 aaacggcacc ctcgaccgcg tgcaggcgta ggcgtcgacc gctgccgccg ggggagcgag
     9001 atggcatgat gcagagatgc tcgatcagca agagatctcc gatcgcctcg aactcgtgga
     9061 cctgatggtt cgctactccc acgccgtcga tacgcggtcg ttcggcgatt tcgaccacat
     9121 cttcaccgag gacgccgtga tcgattacac ggccttcgga ggccctcggg gcaccgtcga
     9181 cgagatcaag gcgtacctca ccgagaccct cgcaccgttc cccacgttcc agcacctggt
     9241 cgcgaacccg ttgctcgtca tcgacggcga cgtcgcctcg ggccgaacca tgtgcttcaa
     9301 cccgatgggt gtcgcccgtc ccgagggcga atcgggcgag ccacgcatgt tcttctgcgg
     9361 gctctggtac gaggaccagt tcgttcggac cggcgacggg tggcgcatcc gcgagcggtt
     9421 cgaggtgaaa tcgtggacgc acaacatggg cccggcgttc tcgggcggcg cagcggcccg
     9481 gaaggactga cgacatggcg accatctggc aactcatcga gcgacgcgcg ggcgaaaccc
     9541 ccgatgacgt gatgctcatc gacgaatccg atcgccgcgt gaccttcggc gagttccgcg
     9601 accaggccgc cgccctcgcc gccggtctgg ccggcgccgg catcggttcg gagagccgag
     9661 tcacctggca gctgccgtcg tccatcgacg ccgcgatcac gatggcggcg ctgagccgcc
     9721 tcgacgccgt ccagtgcccc gtcctccacc tgtaccgcaa gaaggagctg ggcttcgtcc
     9781 tgaagcacac gggcgccgag tactgcatcg tgccgggcga gtggaagggc ttcgactaca
     9841 ccgccatggc cgacgagctc gctgccgacc tcgacccgtc cccggtggtg ctgaccctcg
     9901 gttcactgcc gacgggcgac ccgtccacgc tgccgccggc agccaacccc gccgacggcg
     9961 acgagcccgt ccgctggatc tactccacct ccggcaccac ctcggacccc aagggcgtgc
    10021 agcacaccga cgcgaccctg atcgccggcg ggcagggcct ggccgacgcg ctccagatga
    10081 cgtcggcgga tgttggctcg atcgctttcc ccatcgcgca catcgccggc cccgactaca
    10141 tcatcatgat gctgatcgcg ggcatgtcgg ccgtgatcat cgaggccttc gtgcccgacc
    10201 aggccatcgc gacctacaag aagaacggcg tcacgatggc cggcggagcg accgcgttct
    10261 accagatgtt cctcgccgag cagcgcaagc agccgggcga tccgatcatt cccacgctgc
    10321 gcgccctgtc cggcggcggc gcacccctcc cgcccgagct gtaccacgag gtggtggccg
    10381 agatgggcat caaggtctgc cacggctacg gcatgaccga gatcccgatg atcacgcagg
    10441 ggtcgcccac cgacaacgac gaccagctcg cccacaccac cggcacaccc gtgaccggag
    10501 cggacgtgcg catcgtcgcc gaggacggct cggtggctcc tgccggcgtc gacggtgagg
    10561 tgcgcgtgaa gggcccgatg gtcacgaagg gctacaccaa cccggcgctc accgccgagg
    10621 ccttcgacga cgacggctac ttccgcaccg gcgatgtcgg ccacctccgc cccgacggcc
    10681 acgtcgtcct caccggccgg ctcaaggaca tcatcatccg caagggcgag aacatcgcgg
    10741 ccaaggagat cgaagacctc ctgtacgcgc atcccaaggt cggcgacgtc gccgtgatcg
    10801 ggctgcccga ccgcgagcgc ggcgagcggg tgtgcgccgt ggtcgagccg cccgccggtg
    10861 gcgaggtgat cacgttcgac gagatggtgg cctacctcgg tgagcagggg ctgacccggt
    10921 tcaagacccc cgagcagctc gagatcgtcg atgccctccc ccgcaacgag acgctgcgca
    10981 aggtgctcaa gtaccagctc cgcgacacgt tcgccgccgt cccctggccg tgagtcagga
    11041 gtcctcgacg agcgtggagc gcacggcccg cgcgagccgc tcagcctgag cgagggccag
    11101 caacggcggt tcggtcgggc gatcggcttc gagtcgcacg cggagctgca ccagctcgtc
    11161 gggcagttcc ggccggacct gctcgcggag ctcggcgagc cgccgtcgcg cgtcgtcgcg
    11221 ttctcgccgg atccggccca agtcggtctc gaccgcagcg atcgaggcga gcaacgcggc
    11281 ctgctcacgt cgcgccgcgt cgagttcggc ggcgagatcg gccagctcgt gggtggcgcg
    11341 gatctcctgg accgggtccc agtcggcgtc gtgttccagg tcgtcgacca gcgccagcaa
    11401 gcggtcccgg agcccacggt gttcgacgac ccggagcacg cgccgaagtt cggcgaggtg
    11461 ctgacggcgg tcgtcacgag gggttgaagt tcgacgcacg tgacctcccg gtgaccagcg
    11521 gactccccca cccaacacgc cgcgccgccc cacgtcacgt cacccggcgt cacgtcgccc
    11581 cagcgcgcca gagcgccacc gcgccaaccc ccggctttcg cgggtagttg tgctcgaggg
    11641 gtgggcacaa ctacccgcag aacccggggg tggcgggagg gggcggggga tctagacggc
    11701 gagcagctcg gtcggcgggt gagcggcgtc ccactcgtcg gcctcgtcgg tggcgccggc
    11761 cgggcgcagc gccgcggcca gcacctcgcc gatgtcggcg gcaatgtgga aggtcatctg
    11821 gtcacgcacg gcctcgggga cgtcctcgag atcaccggcg ttgcgcttgg ggagcaccac
    11881 ctcggtcagg ccggcccggt gggcggccag caccttctgc ttgagcccac cgatcggcag
    11941 caccttgccc tgcagcgtga cctcgccggt catacccacg accgggcgca ccgggcggtc
    12001 cgtcagcagg ctgacgaggg ccgtcaccat cgtgacacca gcggacgggc cgtccttcgg
    12061 aacggcaccg gcggggacgt gcaagtggaa gcgacgcttc tcgaaggtca tcggatcgat
    12121 gccgagctca gcggcgtggc tgcgcacgta gctgagcgcg atctccgccg actccttcat
    12181 gacgtcaccg agctggcccg tgatggtgag gccctcgggc ccggccatcg aggtcgcctc
    12241 gacgaacagc acgtcgccgc ccacgccggt cacggcgagg ccggtggcga cgccgggtgc
    12301 gctggtgcgg tcggccgcct cgaagaagaa gcgggctcga ccgagccagc cggcgacatc
    12361 cgcggcgtcg acggtcaccg gggccgtctg ctctcccgac gccagcttgg tggcgacctt
    12421 gcgcagcagc ctcccgacct cgcgctccaa ggagcggacg cccgcctcgc gggtgtagtc
    12481 ggcgacgatg gtgcggagcg cctcgtcggt cagcgtcacc tcgtcgggac ggagcccggt
    12541 ccgatcgagc tggcggtcga ccaggtggtg gcgggcgatg gcgatcttct cctcctcggt
    12601 gtagccgtcg agtccgatga tctccatccg atcgcgcagc gggccgggga tcgtgtccat
    12661 cacgttggcg gtggccacga agagcacgtc ggacagatcg agttcgacct cgaggtagtg
    12721 gtcacggaac gtgtggttct gggccgggtc gagcacctcg agcagcgccg aggagggatc
    12781 gccccggtag tcgccaccca acttgtcgac ctcgtcgagc atcacgacgg ggttcatcgt
    12841 gccggcgtcg ctgatggcgc gaaccaggcg gcccggctgg gcacccacgt aggtgcgacg
    12901 gtggccacgg atctctgcct cgtcgcgcac tccgccgagc gcgacccgga cgaactcgcg
    12961 accgagggcg cgggcgaccg actcaccgag cgacgtcttg ccgacaccgg gagggccgac
    13021 gagggtcagg atggcgccgg cgccccggcc ctcgaccggg gcgagctttc gctcggcccg
    13081 cagcttgcgg acggcgaggt gctcgaggat gcgttccttc acgtcctcca gcccgtcgtg
    13141 atcggcgtcg aggatgcgag ccgcttcggc gatgtcgagg cggtcttcgg aggtctcccc
    13201 ccacggaagt tcgagcaccg tgtcgagcca ggtgcggatc cacccgtgct cggggctctg
    13261 ctcactcgtg cgctcgagct tgtcgatctc cttctcgacg gcgctgcgca ccttgtcggg
    13321 caggtcacga tcggcgatct gggctcggta gtcgtcggtc ggcgacgcct ccgagccgtc
    13381 gagctggccc agctccttct tgatggcgtc catctggcga cggaggagga actcacgctg
    13441 cgtcttctcc attccctcct cgacatcgga gcggatgcgc tggcggatcg acaggtcgcc
    13501 gagggtctcc ttggcccagc cgagcaccag acgcagccgc gcctcgacgt cggtcgtctc
    13561 gagcacctcg accttctgct cgaagtcgag gtcgggcgag tacccggcgg agtcggcgag
    13621 cgcgtcggcg tcggtcacgg gcgggagcat cgcgatgaca cgggcggcac cgcgggtctc
    13681 gaggatgttc tcgatgacag cgcggtactc cttggccaac tcgagggcgg cgtcggacac
    13741 gggttcgggg tcgacgggct cgacctgcac ccacagggcg gcgccggtgc cgggcacgcc
    13801 ggcgccgatg cgagcgcggc gctcgccccg gacgacgagg ccgagcgtgc cgccggggag
    13861 cttgcccgac tcctcgatca ccgaaacggt gccgacgttg gcgtagcgcc cgtcgacgcg
    13921 gggcacgagg agcaggcgac cgtcagcggt cgtggccgcc tcggcggcag cacgggcctc
    13981 gtcggtctcg agcgccatcg tgatcaccat gcccgggagg acgacccccg aggtgagcgg
    14041 gagcaggggc agggtgtgcg acagatccgt gtggtcgaga tcggaacggt cggattcgga
    14101 cggtgttgct tgggacacgg gggcctcccg gagaggtgaa aaggttcaag gcactcaacc
    14161 attgagccag ctcatccatt cccggacgcg actctggagc gctcaggtgc cacttcttgg
    14221 caccttggcg ctccagagtc ggggcggatc tcgattcggt cagaccgcgg ggtgggcgtc
    14281 cgccatctcc cgaagctcgt tcttcgcgac cttgtcgagc gtggccttcg ggaagtcgtc
    14341 gacgaagtac acggcgcggg gcaccttgaa gtcggcgagg ttcgtcttgc acgcggcgat
    14401 gatggcggcg gccagctcgt cgtcgtcctg gttcggatcg gccttcacga cgaacgcgac
    14461 ggcgacctgg tcgagcatct cgtggctctt gcccacgacc gcgacgtcgg cgatgccggg
    14521 cacggtgcgg acctgatcct cgacctcgcg agccgagacg ttctcgccgc cgaccttgag
    14581 ggcgtccttg tcacggtcgc ggtactcgag gttgccgtcg gggccgagca gcacgaggtc
    14641 gcccgtctgc agccaaccgt cggcgttgaa cgccttgttg ttcgcctcct cgttgtcgta
    14701 gtactcgagg aacagctgga tgccgcgggt gccacggagc cagatctcgc cgatctcgcc
    14761 ttccttgacg atctccttgg tctcggggtc gacgacgatc atctcgtagc cgggagcgac
    14821 gcggcccatg gccaggcggg ggtaacgctc gtacttgttg tcgcgcacgg cgtggatgac
    14881 ggtctcggtc atgccgtagg cggcgaccac ccgcatcttc agccagtcga ccaactccgg
    14941 catgatcagg ccgaacacac cgaccttcac cgtgtggtcc gggatcggct ggggagcgac
    15001 ggccttgaac acgaatggga tgagcgagat gtgggtcacc ttgtgcttga ggatgacctc
    15061 ccagaacttg ctggccgaga acttcggctg gaggatcacg gtgccgcccg caccgagcga
    15121 ggtccagatc gaccagctct gcgcgttcac gtggaagaac ggcaggtaga tcaggtagac
    15181 gtcgccctcg tccatgtcga tgttctgcgg gccgaccttg gcggcccaca gcgcgttgcc
    15241 gtgggtgtgc accacggcct tggggcgcga cgtcgtgccc gacgtgaaca ggatgccggc
    15301 ggggagcaac ggatcggggg tgtgacgggg cgcttcgggg aggtcgtgct cgaggagctg
    15361 gtcgaagccg tcgtggggtc gatcgacggg ctcggcggtc gcggcgatgc ccgagtcgtc
    15421 ggcagtgacg acgaaccagc cgaggtccgg caccgcctcg ttgaccacgg cggcgaactg
    15481 cggctgggtg atggcgccga cacacccggt gtgggtggcg aagtagttga tctcggcggc
    15541 gacgctgcgg gtgttggtcg tcacgccgac agcgccgatc cgggcacagc cgtaccaggc
    15601 gagcaccgcc tcggggcagt tctcggcgtg gatgagcacc ttgtcgccga gcttcacacc
    15661 acgagccacg agggccgagg cgaccttctc gacgtcatcg acgaactggc ggtaggtcca
    15721 ggtgcgatcc tcgcccgacc gcggttccca gacgaggaac ggacggtcgg gggccgcggc
    15781 cgcccagtgg ttcagcagcc atgggatgtc ctggcccacg aactggaacg cgtcgatttc
    15841 cgcttgctgc atcgctaccc ctcgagtcga tctgacgatg tgtcagatac agcttcgcgc
    15901 agccaggggc gggacgcgtc agcgggcccg gccctcccgc ccaccagctc cctccaggtg
    15961 gtgtgcacac ctggagggag cgggccgccg aatcggcggg ctgggtgttc accggatcgg
    16021 acggggttgg aaccgaggtg tccgaccaca gtgagaaagg gagatgggat ccctacccac
    16081 aatgctgaca agttgtcatc attgtgtgcc gaagcatcgt gccacgtcag gagcgccacg
    16141 tgtagtggca cccctgttca actttttccg tactccaccg ccgggactga tttgaatttc
    16201 aatgtcacac gccccgcacc aacgcgaaca ccaactgtga cgaccaccgc ccccacacct
    16261 cgaggacggc tgagctaccg ggtcggagac gaccaggccg aacgctgcca agtgccgctg
    16321 tacgacgatt cggtcgtgac cttcggccgc ggggagtcgt gtgaggcccg gttcggacac
    16381 gcacccgtgc gggactggga gatccctcgc gtggcgggct ggctcgtcat cgcccacgga
    16441 cggatcttcg tcgaggcagc gggcatgccc gacccccacc ggggagaagc cggccgtccc
    16501 cagccagtgc gtcgagcact ccaggtgagc gccgcaggtg ggccacccgt cccggtcgcg
    16561 gccggttcgg cctattctcc gtcggaggag atcttcacga tcgaggtcgt cggcgccact
    16621 ggcacctggg agctcgacgt cgtcgcccgc tcccgcgacg agggcgagga cccgaccgtg
    16681 cacgagcccc acacccacag cgtggtcatc gacctgaccg acgtccagcg tgaggtgctc
    16741 ttggcgtacg ccgaaccggt gctcgcggga tcggtcgagc cggcaaccca cgatcaggtt
    16801 gccgccaagg tgtacatgag ccgatcccag gttcgacgcc acctggaacg gctgagcgac
    16861 gagttcttcg ccaagcgcct ctggagcccc gactcgggcg atacccgggt ccgagtcgtc
    16921 gagaccgcac gccacaacca catcctcacc gagtcggccc gacggcactc cgacgccacg
    16981 tcgccggcag cggaccggcc agccgggcat tgaccgagca gctctggacg gggcccgcgg
    17041 acgacccccg tcgttaccgt gtgccactcg tcgacggccg cccggtgccc gaacccggtg
    17101 gcggcggcga cggcctcgtc taccgggcaa ccgaccatga gggcaacgac gttgcgctca
    17161 agctgctcct ccatgcccgc gccgccgacc tccccgacgc ggccgttcgg ctcgaacgga
    17221 tcgccgtact cgccgaaccg catctcatgc gtccgatcga ggtgttcgtc ggcaccgcgc
    17281 tgaccaacga ccccgagccc gacatcgagg aggccgacgt ctgttggatg gccgccgaat
    17341 gggtggacgg tcggcgactc gaacacgtcg cgaccgacga cgaccccgag cagaaccttc
    17401 gccacgtcgc ccagatcgcc cggggtgtcg cggcgctgca ccgagcgggc ggcatcgtgc
    17461 accgggatgt gaagtcgggc aacgtccggg tcactcccga cggccgggcc gtgctcatcg
    17521 actacggctc accgggccgg ccgtcgagcg acgtgcccgc gggcacaccc ggttggctgt
    17581 ctcccgaggt cgccgccggt cacgctcccg gccacgccgc cgacgtctgg ggcgtcggcg
    17641 cgatcgccca ccacctgctc gtcgggtccc cgccgcgcct cgacggtgcc gcatgctcgc
    17701 gggaacgact ccagttcatg ggagcacgaa ccggcctccg cgacgccgac gaactggcga
    17761 cgcacatctc ccgcctgctc gagagtgaac ccggacgccg tcccgacgac ctcgaccggt
    17821 gggccgaccg actcgacgcc gtcctcgacg gccgaggccg gtcacaccgg cgacgactgg
    17881 tcgcagcgac cggggtcgtc gcgatcgggg cgctcgccct gatcgccgtg gtcggtggca
    17941 acgatgaccc cgagaccccg accgcagccg cgtcggcgtc ggcgtcggcg tcgat
//
LOCUS       DAOSOA010000019        17902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3173_length_17902_cov_6.363871, whole genome shotgun sequence.
ACCESSION   DAOSOA010000019 DAOSOA010000000
VERSION     DAOSOA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17902
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3173_length_17902_cov_6.363871"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKY13_02665"
     CDS             complement(<1..466)
                     /locus_tag="PKY13_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT78857.1"
                     /translation="MSYRFESPADVRTRLGGVDYLSDDAIAGVVYLADRLGKPILVEG
                     PAGTGKTQLAKSVAEIIGARLIRLQCYEGLDESKALYEWNYKKQLLRIQATKTEGDTA
                     DWNQIEEDIFSDDFLLTRPLLEAIRADDPVVLLIDEVDRVEVETEALLLEILS"
     gene            543..1310
                     /locus_tag="PKY13_02670"
     CDS             543..1310
                     /locus_tag="PKY13_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT78858.1"
                     /translation="MDYSEMSSPPDEFDIDLDLSSLDPLPTGSVLQKRPSRGASAGQN
                     ERREAGPAGVASDEPLVDAGRDLLCVNCYHRAGWAGTSSTTWLRRGVVARLLESVQLD
                     LPDGFGLAVYDGWRSPQTVTALYDHFYGEGSDLEPGYLAPPSKDPDDPDNVDDIDPPP
                     HLTGGAVDLTLSWRGTPLSLGTPFDEFTPRAHLAALESGPATLDRDLRRLLFTVMTEA
                     GFAPYEQEWWHYSYGDRAWADHHGHAAPLYGATSPNR"
     gene            1321..>2633
                     /locus_tag="PKY13_02675"
     CDS             1321..>2633
                     /locus_tag="PKY13_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT78859.1"
                     /translation="MIASDALAPMVAAFVDDVTVALTDAASRVRPPRTGDFRADVVNE
                     AFNLCVAFIDVDDRHTDNELWALTSTFGPLIDIAGLAGATPAQLRSSTLIAGRKVWLD
                     RMSTLFDILVAADSVDGGERSMTYYRRALDICHLIAALDEITAQAELTAIGDYRNLLL
                     NHIRMRVPADRQPDVTKVEPHIPETADPAGPPPELPPEPPARPIEELLAELDSLVGLS
                     EVKAEVRLVTDLLRVQQLRKERDLPTIDTSLHLVFVGNPGTGKTTVARLLAQIYHSVG
                     ALTRGHLVETDRSGMVAGFVGQTAPLVVKRFDEADGGMLFVDEAYTLVRGGDNDFGRE
                     AIDQMVKLMEDRRDRTAVIVAGYPDEMADLIAANPGLKSRFPKTISFPDYSTDELLAI
                     FESICGKQRYELDEASTERLRDVLDAEPRDKGFGNGRFARNLFEAA"
     assembly_gap    2634..2733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2734..3147
                     /locus_tag="PKY13_02680"
     CDS             <2734..3147
                     /locus_tag="PKY13_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78860.1"
                     /translation="LTGWPDLEDPGTGDIEANDRFVQELIGLKAKRVESSDPLTTILQ
                     LPKFEVALDLDVDRRLGGAARRNDFTATPTGITAVAVAVPAATTAGRAALERLDQAKL
                     ESALTDQGWSTNLPDDDGLPDPGVLDALASQWSPA"
     gene            3144..4985
                     /locus_tag="PKY13_02685"
     CDS             3144..4985
                     /locus_tag="PKY13_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT78861.1"
                     /translation="MNPRPSVSRRFAVALAGVTLMASACGGGDDSSGPGVTSAFDDTG
                     DCGIVVDMSVSSEKIDVIKALADSFNAEKRKVGDTCVFVRPQSKASGGAATLLSTDWD
                     TAAEGPEPVIWSPASAAWGAVLNQRLADQGQPEMAPPDSTPFMLTPLVIAMPKPMATA
                     LGYPEKPVGWADIARLATDPAGWAAYGHPEWGPFRLGKTNPNFSTSGLSALIAQTYAA
                     AGKTTGLSTEDLANPAVVDFSKQIESSVVHYGDITMTFLNNWFKADRRGTSLTYASAV
                     AIEEKSVVDYNKGNPDGVLSPGEKPRVPKVPLVSIYPSEGTLYSDSPFYVLDAPWVDA
                     DERAGAQLFGEFVQQPENQSKVLEYGFRPANPAVKVADPIVKANGVDPDQPATLLDVP
                     QPSVMIDLLDAWAKNRKTARVLMVLDVSGSMGQPADGNDGDSKLDLAKEAAIDALDQF
                     NPEDEVGLRVFTTNMGPGQDEEFQDLQPIAPIGANRERLAESIRNLIPQNATPLYSVA
                     QASYDEMFQSYDPTRINAVILLTDGANEDANPEDDQQQQETLLENLRKGSSGELSKPI
                     RMFTVAYGADAQTDALSLIADASSAAAYSAKDATTIAKVFTQVVSNF"
     gene            4985..5839
                     /locus_tag="PKY13_02690"
     CDS             4985..5839
                     /locus_tag="PKY13_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78862.1"
                     /translation="MLSFRDRFFTRKVSTAMTSPLAIVAAGGGAAAGILAGGAAGPVG
                     AVVGGIIGAIVAWSARVGLAVPKAPKKDRIDPFTIAEPWRRLTQDAIAAKRQFNDAVG
                     RTSPGPIRDRLTTIGERIDDSVEEAWAAARAGHELTDAYSRLDVASTQRELEAINATA
                     AAPRSAATQATVTALEAQLATARRMYQMITDTREGLQLLNARLDESVAHSIELSVGAY
                     RPEAFNEVEGDLGSITDELEALRGALEETSSSRLPTLPEPGPSAIAGVVEGQAGEGAA
                     GEARPQAQ"
     gene            5836..7482
                     /locus_tag="PKY13_02695"
     CDS             5836..7482
                     /locus_tag="PKY13_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOT78863.1"
                     /translation="MTDDVEATAVAAEGVGTSTRRAGRESIWVAAGTGLSRLTGFARV
                     FVLAYAVGQSPLADLYTKANSAPNLVFELVVGGVLSATLVPLFVRSREEDDPESTSVL
                     ISVGFVAAALLSLLALAATFAVDHYLHGRFTAEGGDNASTLVKLQIVTDLLYLMLPQI
                     LFYAIASLSTAYLNASRRYRTAAFAPVLTNLVTIVAFAIVGVQLQGDDVVLEAGQRDL
                     SAVLWLGLGTTAGIAAMVIPMMWAVRNSDDELRFLPRFGHPVVKRVVRLSGWTFGYVI
                     ANQLALLAVVLLLSDNKRYNDAFIFFQLPHGLIAVSIMTTMTPELASAAARRDFQGLA
                     DRFARGLRLLAALIVPAAVGYVLLATPITSFLRHGNLTAEDARLTGRAVAAFAVGLPA
                     FSTYLFACRGFYALSDTRTPFFLNVVENGINVLGIVGLVIVGSASSTTFALAYSVAYV
                     LAAILAVWRFDRRLRELDPDVVRPDLSPLRSMTVAALGMGGVVWLIRQLLPDDAGAGA
                     VASVALGVVLGGLTYLGLGIVLRVPEITAVWAGARRRFLR"
     gene            7582..8379
                     /locus_tag="PKY13_02700"
     CDS             7582..8379
                     /locus_tag="PKY13_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOT78864.1"
                     /translation="MLKTLKKWWKYIGAKLNMSFDEKADPKVQLEQAITEAQEQHRRL
                     KEQAASVIANQKTTEMQLNRKLEELEKVGANARQAVLMVDEATKAGNAAKAAEYTSAA
                     EAFANRLIALEREVEGLKTLALQGAQASDQAKAAVQQNSAALQKKLAEKQKLLSQLDQ
                     AKMQEQLNKALGSLSEVVGQDVPTFDEISRKIESRQAKAMAGAELEGSSVEAKMLEVE
                     QASINVEAQARLEEIRSQMGLSTAPADAPEAAPATEPATGGEAGAAG"
     gene            complement(8453..9520)
                     /gene="proB"
                     /locus_tag="PKY13_02705"
     CDS             complement(8453..9520)
                     /gene="proB"
                     /locus_tag="PKY13_02705"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOT78865.1"
                     /translation="MIVVVKVGTSSITDVDGRIDRSAIAKVAGDVVRSRQAGHEVVVV
                     SSGAIAAGLPALGLTARERPKDPRTLQAVSAVGQAHLMATWNECFAEHDVVAGQVLLV
                     PDDLNRRSTYLQARATLGRLGELGVVPVINENDAIADDEIRIGDNDRIAALVAHLIRA
                     DLLVLLTDAPGLLTADPSVDEHASLIEDVIEVDQELESLAGGSGSNRGSGGMATKLAA
                     AKIATWSGVRTVIAKASRPDVLIDAIDGVQGVGTIFRPAGRRLSARKVWIAFAVGARG
                     RLHVDAGAVQALLSDKSLLAPGIRKVEGSFHRGDAVEIVGPDGTVCGKGTVRLDASQI
                     DGGVGRGVAVHKDDLIILDLS"
     gene            complement(9555..10847)
                     /gene="obgE"
                     /locus_tag="PKY13_02710"
     CDS             complement(9555..10847)
                     /gene="obgE"
                     /locus_tag="PKY13_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT78866.1"
                     /translation="MSNFVDEAGLNVKGGDGGAGCVSFRREAHTPKGGPDGGDGGSGG
                     DVWLVADRNVASLLAFRDHPHRKATGGTHGSGAKKHGSTGKDLMVPVPEGTIVKDRDG
                     TVLADLVQQGDRWLAAHGGQGGKGNTRFMSSRRRAPSFAEQGEVGEERWMWLELKLMA
                     DVAIVGLPNVGKSTLISRVSAAKPKIADYPFTTLVPNLGVVRTDDGFEMVLADIPGLI
                     EGAAEGKGLGHQFLRHIERARVLLLILDLSSTAEQTPLEQEAVLLDELERYRPELIER
                     ERVRIGARADLATPDTLLEFNGHIVSSVTGLGIPELIGRLRVAVEEARAATAMPDAYV
                     VHRPTPEGIRIERDESGEFEVHGREALRAVGVSDLTNAEALDYVHERLSSLGVDRALA
                     RAGARNGDVVHIGKLSFEYENADEINFADEAEDRGRKQ"
     gene            complement(11050..12597)
                     /locus_tag="PKY13_02715"
     CDS             complement(11050..12597)
                     /locus_tag="PKY13_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOT78867.1"
                     /translation="MIPRRARRFLPSLPLGRRRRRDEKGYVLAVSAIIMVPLLAISGM
                     ATDIGGWYAEGSKMQKAADAAALAGVVWLPDLSKATAVANETARQNGFDSSKANISIV
                     VGQISNTDLRVSITDTDAPIFFSRFFMSSMTIKRESIATYVLPVPLGSPRNFFGTGNL
                     YTSEPEGMWASINGWCAPKEQGDPFAVGFEGNWPATGQVCPGSTANGQYRANPTSQYE
                     YIISVPAVRALPIVVHLYSPAKTTSSPDNNDGTNMTTTFTLRSPDNTPFDDDDNPITQ
                     CTPIVQSNPRAYAPNEVDNDATILGVSGWSAFCTIPTSAVGGNYVLGVRTLQGELGSN
                     GSNQYAVMASYNGTGTACDSRTDATCPTVVGRNWISVLATSTSSNANFYLAEINPIHA
                     GKQMEITLFDPGEGGNYIQILDPNGFPVNFSYRTADGASGLTGSGTQLDVSGCNASMT
                     QIGPNRASNCKFNERFVILTVDLPANYATAYAARWWKINYVFTSAVTDRSTWSVRIIG
                     DPVHISN"
     gene            complement(12594..13166)
                     /locus_tag="PKY13_02720"
     CDS             complement(12594..13166)
                     /locus_tag="PKY13_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOT78868.1"
                     /translation="MLVEVVLILPLLMLISLAVFDLGLGWKASMTVTNAARAGARVAS
                     NLGVAATADKSALASIAASLGTVPVSEIDVIVIYRATTADGDVPADCLTGSTRATGGS
                     SASQCNVYTGAEAIAASTSTTFTGNCGSSRDRFWCPSTRGNSQAASTGPDYLGVYVRI
                     NHTTKTKAFGSTMVVKDRAIMRIEPNAGNP"
     gene            complement(13226..13834)
                     /locus_tag="PKY13_02725"
     CDS             complement(13226..13834)
                     /locus_tag="PKY13_02725"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOT78869.1"
                     /translation="MTGGEALRFRHERSLTRSERGAVVVESALALPLFLLLIFGTMEF
                     GLAFRSYLTLANATRDAARFASTLGKDADADFQIVNEITGNLSGFKTGAIKKIIIFKA
                     TGPTSTTSSGALAACRTGSVTGLCNTYIAGSISTNSANYGCNYTSPDRFWCPAGRKVA
                     VSDPPDYIGVYIETRHTGVTGVIGMTRDFSDEFVMRLEPVRA"
     gene            13958..15190
                     /locus_tag="PKY13_02730"
     CDS             13958..15190
                     /locus_tag="PKY13_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT78870.1"
                     /translation="MSKSAVPHDTDPVAEPRPVGSRRAEQWAATMAGHGQPAGGEAPV
                     ALRHAHREGFNTIGTRPIADRQAREELERATLTEGATLAVGAGTRARPEAPDPLRTCF
                     ERDRDRILHGTTAFRRLAGKTQVFIFPEDHQRTRLTHALEVAQVATAIAGAVGLNVVL
                     TEAIALGHDCGHGPGGHASEDAFSPYVPGGYDHAVWGADVVLEPLNLCVETLDGIRNH
                     SWSRPTPLTPEGEVVSWADRIAYVCHDFEDAVHAGIVSPDMLPEVVRDRCGATRSEQL
                     GSFIRAMVETIARTGHIALDEPTADALAAFRRFNYERIYMRPASVAQSESVIRVLRAL
                     VEYYADRPNTLLLDDPHHRPAHQPAVVRPHAAAGSPEALHDAVSYVAGMTDRFAFNAA
                     IGLLGWDRDTLPQGVGIR"
     gene            complement(15258..15509)
                     /gene="rpmA"
                     /locus_tag="PKY13_02735"
     CDS             complement(15258..15509)
                     /gene="rpmA"
                     /locus_tag="PKY13_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT78871.1"
                     /translation="MSKTKGGGSTKNGRDSESKRLGVKVFDGGVVNAGSIIVRQRGTK
                     FYPGVNVGRGGDDTLFALAPGKVKFGSRKGKKLVDVLTD"
     gene            complement(15516..15824)
                     /gene="rplU"
                     /locus_tag="PKY13_02740"
     CDS             complement(15516..15824)
                     /gene="rplU"
                     /locus_tag="PKY13_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOT78872.1"
                     /translation="MYAVIKTGGKQYRVAEGDRVQVERLGEPGTDVSLTPVLLVDGDN
                     TLATPAQLKGAAVSARIVEETKGPKIIGFTYKNKSNNRRRWGHRQTLATIEITGITKG
                     "
     gene            complement(15889..17643)
                     /locus_tag="PKY13_02745"
     CDS             complement(15889..17643)
                     /locus_tag="PKY13_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOT78873.1"
                     /translation="MSETEPTTPPTSAPLVPKVPPPAPRKPQIGDSIPGGAPAAAGGQ
                     PSAPGEGLSTSAKRRRRRSRGGGGGGGEGRDNGGRSPQDGGQGGGQGGQRGGGQARGG
                     QGQGQGGGRNGGGGGQRGGQKRPAATPIEAVLTDDDGPVHLDAKALKKRQGRQRNGRA
                     VGRYQMNVHVDSAGHTHIAVLEGRSLIEHYVSRPSDDVSQIHGNIYLGRVENVLPGME
                     AAFIDIATPKNAVLYRGDVHLDADDVEVKADARIEDVLKPKQLILCQVTKNPIGHKGA
                     RLTQEVSLPGRFVVLVPNSKTYGISKRLSDQERKRLRQILDRVKPAHHGVIVRTAAEN
                     VTSEEIERDVTRLLKQWDQISALAAKQNRPALLYREPDMAVRVIREEFNEEYRAVIID
                     EPGLYSEVHDYVAAISPATADRVQHYDLETENLSLFERFQITEQLKKGLDRKVWLPSG
                     GSLIIEHTEALTVIDVNTGRNVGSSSLEETVYRNNLEAAVEIARQLRLRDIGGIIVID
                     FVDMEIKANRDELVRTFKEALARDKTRTQVFSVSDLGLVEMTRKRIGEGLLESFADQC
                     SVCNGRGIVLTSAVVGID"
     gene            complement(17657..>17902)
                     /locus_tag="PKY13_02750"
     CDS             complement(17657..>17902)
                     /locus_tag="PKY13_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78874.1"
                     /translation="RPALLSATTQPALREGAVVLTVDLATKPRSIRPSEFLTCLEQAG
                     EVRRICRNEQWMTIDGTSLPPLVAGDPSQPVGERLAS"
BASE COUNT         2766 a         6262 c         5974 g         2800 t
ORIGIN      
        1 cggagaggat ctcgaggagc agcgcctcgg tctcgacctc gacgcggtcg acctcgtcga
       61 tgagcagcac gaccggatcg tcggcgcgga tcgcctcgag gagcgggcgg gtcaggagga
      121 agtcgtcgga gaagatgtcc tcctcgatct ggttccagtc cgccgtgtca ccctcggtct
      181 tcgtggcctg gatgcggagg agctgcttct tgtagttcca ctcgtagagc gccttggact
      241 cgtcgagccc ttcgtagcac tggaggcgga tgaggcgggc cccgatgatc tcggcgaccg
      301 acttggcgag ctgcgtcttg cccgtgccgg caggaccctc gacgaggatc ggcttcccga
      361 ggcgatcggc caggtacaca acgccggcga tggcgtcgtc ggacaggtag tcgacgccgc
      421 ccaggcgcgt ccggacgtcg gcgggagatt cgaagcggta ggacatcggg ccagactacc
      481 gacgctcttg accgcctggt caatcgcatt tccggtcggg acggggtcgc taccctcacg
      541 gcgtggacta ttcggagatg tcgtcacccc ccgacgaatt cgacatcgac ctcgacctct
      601 cctcgctcga ccccctcccc accggttctg tcctgcagaa gcgcccctcc aggggcgctt
      661 ctgcaggaca gaacgagcgg cgcgaggcag ggccggcggg ggtcgcgtcc gacgagcccc
      721 tggtcgacgc cgggcgggac ctgctgtgcg tcaactgcta ccaccgcgcc gggtgggcgg
      781 gcacgagttc cacgacgtgg ctgcgccgcg gcgtggtcgc ccgactgctc gagagcgtcc
      841 agctcgacct tcccgacggg ttcgggcttg cggtgtacga cggatggcgt tccccccaga
      901 ccgtcacggc gctctacgac cacttctacg gtgagggctc cgatctcgaa ccgggttacc
      961 tcgctccccc gagcaaggac ccagacgacc ccgacaacgt cgacgacatc gacccgcccc
     1021 cgcacctcac cggtggcgcc gtcgacctca cgctctcgtg gcgggggacg cccctgtcgc
     1081 tcggaacccc cttcgacgag ttcacacccc gggcccatct cgccgccctc gaatcggggc
     1141 cggccaccct cgatcgcgac ctccgacgcc tcctcttcac ggtgatgacc gaagcgggat
     1201 tcgcacccta cgagcaggag tggtggcact actcctacgg cgatcgggcc tgggcggacc
     1261 atcacggcca cgccgcgccc ctctacgggg ccacctcacc caaccggtag cctcggcgca
     1321 gtgatcgcct ccgacgccct cgcaccgatg gtcgccgcgt tcgtcgacga cgtgacggtg
     1381 gcgctcaccg acgcagcgtc gcgcgtccgc ccaccgcgaa ccggcgactt ccgggccgat
     1441 gtggtcaacg aggccttcaa cctctgcgtc gcgttcatcg acgtcgacga ccgccacacc
     1501 gacaacgagc tgtgggcact gacctcgacg ttcggcccgc tgatcgacat cgcaggcctc
     1561 gccggagcca ccccagccca actccgcagc tcgacactca tcgccggccg caaggtgtgg
     1621 ctcgaccgga tgtcgacgtt gttcgacatc ctcgtcgccg cggacagcgt cgacggcggc
     1681 gagcgatcca tgacgtacta ccggcgggcg ctcgacatct gccatctcat cgccgcgctc
     1741 gacgagatca ccgcgcaggc cgaactcacc gccatcggcg actaccgaaa cctgctgctc
     1801 aaccacatcc gcatgcgggt gccggccgac cgccagcccg acgtgaccaa ggtcgagccc
     1861 cacatccccg agacggccga tcccgccggc ccgccacccg aactcccgcc cgagccgccc
     1921 gcccgcccga tcgaggaact gctcgccgaa ctcgattcgc tggtggggct cagcgaggtg
     1981 aaggccgagg tgcgcctcgt gaccgacctc ctccgggtgc aacagctccg gaaggaacgt
     2041 gacctcccca ccatcgacac gagcctccac ctcgtgttcg tgggcaaccc cggcaccggc
     2101 aagaccaccg tcgcccgcct cctggcgcag atctaccact cggtcggggc cctcacccgg
     2161 ggccacctcg tggagaccga ccgctcgggc atggtggccg ggttcgtcgg acagaccgcc
     2221 ccgctcgtgg tgaagcgttt cgacgaggcc gacggcggca tgctcttcgt cgacgaggcc
     2281 tacacactgg tgcgcggcgg cgacaacgac ttcggtcgcg aagcgatcga ccagatggtg
     2341 aagctcatgg aggaccgtcg cgaccggact gccgtgatcg tcgccggcta tcccgacgag
     2401 atggccgacc tgatcgccgc caaccccggc ctcaagtcca ggttcccgaa gacgatctcc
     2461 tttcccgact actcgaccga cgagctgctc gcgatcttcg agagcatctg cggcaagcag
     2521 cgctacgagc tcgacgaggc gtcgacggaa cggctgaggg acgtcctcga cgccgaaccc
     2581 cgcgacaagg gcttcggcaa cggccgcttc gcccgcaacc tcttcgaggc cgcnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgaccg gatggcccga cctggaagac
     2761 cccggcaccg gcgacatcga ggcaaacgac cggttcgttc aggagctcat cggactcaag
     2821 gccaaacgag tcgagtcgtc cgatcccctg accaccatcc tccaactgcc caagttcgag
     2881 gtggcgctcg atctcgacgt cgaccgtcgg ctcggcggag cagctcgccg caacgacttc
     2941 accgcgacgc cgaccggcat cacggccgtg gccgtcgccg tcccggccgc cacgacggcg
     3001 ggccgtgccg cactggagcg cctcgatcag gccaagctgg agtccgcact caccgaccag
     3061 ggctggtcga ccaacctccc cgacgacgac gggctccccg atcccggggt cctcgacgct
     3121 ctcgcctccc aatggagtcc cgcatgaacc cccgtccctc cgtctcccgc cgattcgccg
     3181 tcgcgctcgc cggtgtcacc ctcatggcca gcgcctgcgg gggcggtgac gactcctccg
     3241 ggccgggggt gaccagcgcg ttcgacgaca cgggcgactg cgggatcgtc gtcgacatgt
     3301 cggtgtcgtc ggagaagatc gacgtcatca aggcgctggc cgactccttc aacgccgaga
     3361 agcgcaaggt cggcgacacg tgtgtgttcg tccggcccca gtccaaggcc tcgggcggcg
     3421 ccgccaccct gctctcgacc gactgggaca cggcggccga aggcccggaa ccggtcatct
     3481 ggtcgccggc gtcggccgcg tggggtgctg tgctcaacca gcggctcgcc gaccagggcc
     3541 aacccgagat ggcaccgccc gacagcacgc cgttcatgct cacgccgctc gtcatcgcga
     3601 tgcccaagcc gatggcgacc gctctcggtt accccgagaa gcccgtgggg tgggccgaca
     3661 tcgcccgcct ggccaccgac cccgccggtt gggctgccta cggacacccg gagtgggggc
     3721 cgttccgcct cggcaagacc aaccccaact tctcgacgtc gggcctctcg gcgctcatcg
     3781 cccagaccta cgccgcggcc ggcaagacca ccggtctgag caccgaggat ctcgcgaacc
     3841 cggcggtcgt cgacttctcg aagcagatcg agtcgtccgt cgtgcactac ggcgacatca
     3901 cgatgacgtt cctcaacaac tggttcaagg ccgaccgccg cggcacgtcc ctgacgtacg
     3961 cgtccgcggt cgccatcgag gagaagtcgg tcgtcgacta caacaagggc aaccccgacg
     4021 gtgtgctcag cccgggcgag aagccacggg tcccgaaggt cccgctcgtg tcgatctacc
     4081 cgtcggaggg caccctctac tccgacagcc cgttctacgt gctcgacgcg ccgtgggtcg
     4141 acgccgacga gcgcgccggc gcccagttgt tcggcgagtt cgtccagcag cccgaaaacc
     4201 agtccaaggt gctcgagtac ggcttccgcc ctgcgaaccc tgccgtcaag gtcgccgatc
     4261 ccatcgtgaa agcgaacggg gtcgaccccg accagcccgc aaccctcctc gacgtgccgc
     4321 agccgtcggt gatgatcgac ctgctcgatg cctgggcgaa gaaccgcaag accgcgcgcg
     4381 tgctgatggt gctcgacgtg tcgggctcga tgggccagcc cgccgacggc aacgacggtg
     4441 actccaaact cgatctggcg aaggaagcgg cgatcgacgc gctggaccag ttcaaccccg
     4501 aggacgaggt cggactccgg gtgttcacca ccaacatggg cccgggacag gacgaagagt
     4561 tccaggatct ccagccgatc gcgccgatcg gggccaaccg cgagcgcctc gccgagtcga
     4621 tccgcaacct cataccccag aacgccactc cgctctattc cgtcgcgcag gcgtcgtacg
     4681 acgagatgtt ccagtcctac gaccccaccc ggatcaacgc cgtcatcctc ctcaccgacg
     4741 gggccaacga ggatgccaac cccgaggacg accagcagca gcaggagacg ctgctcgaga
     4801 acctccgcaa ggggtcctcg ggcgagctgt cgaagccgat ccgcatgttc accgttgcgt
     4861 atggcgcaga cgcccagacc gacgcgctgt cgttgatcgc cgacgcgtcg tcggccgccg
     4921 cctacagcgc caaggacgcc acgaccatcg ccaaggtctt cacccaggtg gtgagcaact
     4981 tctgatgctc tcgttccgcg accgcttctt cacccgcaag gtgtccacgg ccatgacgtc
     5041 accgctcgcg atcgtcgcgg ccggtggcgg cgccgctgcg ggcatcctcg ccggaggagc
     5101 ggccggcccg gtcggtgccg tcgtcggcgg catcatcgga gcgatcgtcg cctggtcggc
     5161 ccgcgtcggc ctcgcagtgc cgaaggcgcc caagaaggat cggatcgatc cgttcacgat
     5221 cgccgagccc tggcgtcggc tcacccagga cgcgatcgcg gcgaagcgtc agttcaacga
     5281 cgccgtgggc cgcacgagcc ctggtccgat ccgcgatcgc ctcacgacga tcggcgagcg
     5341 catcgacgat tcggtcgaag aggcctgggc ggcagctcgc gccggccacg agctcaccga
     5401 cgcctactcc cgcctcgacg tcgcgtcgac ccagcgcgag ctcgaggcca tcaacgcgac
     5461 agcggcagcg ccgcggagcg cggccaccca ggccacggtg acggcgctgg aggcccagct
     5521 cgcgacggcg cggcggatgt accagatgat caccgacacc cgcgagggtc tgcagctgtt
     5581 gaacgctcgc ctcgacgagt cggtggcgca cagcatcgag ctgtcggtcg gcgcataccg
     5641 tcccgaggcg ttcaacgagg tggaaggtga cctcggctcc atcaccgacg agctcgaggc
     5701 gttgcgcgga gcgctcgagg agacgtcgtc gtcgcggttg cccacccttc cggagccggg
     5761 gccctcggcg atcgcgggag tggtcgaggg gcaggccggc gagggtgcgg ccggcgaggc
     5821 ccgcccgcag gctcagtgac cgacgacgtc gaggccacgg ccgtggcggc cgagggggtc
     5881 ggcacgtcca cccgtcgcgc cggacgcgag agcatctggg tggccgccgg caccgggctg
     5941 agccgactca ccgggttcgc ccgcgtgttc gtcctcgcct acgccgtcgg ccagtcccca
     6001 ctcgccgacc tctacaccaa ggcgaactcc gcgccgaacc tcgtgttcga gctcgtggtc
     6061 ggcggggtgc tgtcggcgac gctcgtcccg ctgttcgtcc ggtcgagaga ggaggacgac
     6121 cccgagtcga cgtcggtcct gatctcggtc gggttcgtcg ccgccgcgct cctgtccctc
     6181 ctggccctgg ccgcgacgtt cgcggtcgac cactacctgc acggccggtt caccgccgag
     6241 ggcggcgaca acgcgtccac gctcgtcaag ctgcagatcg tcaccgatct gctgtacctg
     6301 atgctgccgc agatcctctt ctacgcgatc gccagcctgt cgacggccta cctgaacgcg
     6361 tcgaggcggt accgcaccgc tgcgttcgcg ccggtcctga cgaatctcgt gaccatcgtc
     6421 gcgttcgcca tcgtcggggt gcagctccag ggcgacgacg tggtcctcga ggccgggcag
     6481 cgcgacctgt ccgccgtcct gtggctcggt ctcgggacga ccgccggcat cgcggcgatg
     6541 gtcatcccga tgatgtgggc ggtccgcaac agtgatgacg agctgcgctt cctgccccgg
     6601 ttcggtcacc cggtcgtgaa gcgggtcgtc cgcctctcgg gctggacgtt cggctacgtg
     6661 atcgccaacc agctcgcgct cctcgccgtc gtgctcctgt tgtcggacaa caagcgctac
     6721 aacgacgcgt tcatcttctt ccagctcccc cacgggctga tcgccgtgtc catcatgaca
     6781 acgatgacgc ccgagctcgc gagcgccgcc gcccgccgtg acttccaggg cctcgccgac
     6841 cggttcgccc gagggttgcg tctgctggcg gcgctgatcg tccccgccgc ggtcgggtac
     6901 gtgctgctgg caaccccgat cacttcgttc ctgcgccacg gcaacctgac ggcggaggac
     6961 gcccgtctga ccggtcgggc ggtcgcagcc ttcgccgtgg ggctcccggc gttctcgacc
     7021 tacctgttcg cctgccgagg gttctacgcg ctcagtgaca cccggacgcc gttcttcctc
     7081 aacgtcgtcg agaacggcat caacgtgctc gggatcgtcg ggctggtgat cgtcggctcg
     7141 gcctcgtcga cgacgttcgc cctcgcgtac tcggtggcct acgtgctggc ggcgatcctg
     7201 gccgtctggc ggttcgaccg gcggctccgc gaactcgacc cggacgtggt tcggcccgac
     7261 ctgtccccgc tccgctccat gaccgtcgcc gccctcggga tggggggggt cgtgtggctc
     7321 atccgccagc tgctccccga cgacgccggc gccggagcgg tcgcgagcgt cgccctcggc
     7381 gtggtgctcg gcgggctcac ctacctcggg ctcggcatcg tgttgcgcgt cccggagatc
     7441 actgcggtct gggccggtgc tcgccgccgc ttcctccgct gacaccaccc gtgaccgtcc
     7501 cggctccgcc ggggccaact acacccaccc acaccacccg tgacggtccc gactccgtcg
     7561 gggccaacta cgattgccac catgctcaag acgctcaaga agtggtggaa gtacatcggc
     7621 gcgaagctga acatgtcgtt cgacgagaag gccgatccga aggtccagct cgaacaggcc
     7681 atcaccgagg cgcaggagca gcaccgtcga ctcaaggagc aggcggcgag cgtcatcgcc
     7741 aaccagaaga cgaccgagat gcagctcaac cgcaagctcg aggaactcga gaaggtcggc
     7801 gccaacgccc gccaagcggt cctcatggtc gacgaggcca ccaaggccgg caacgccgcg
     7861 aaggcggccg agtacacgtc cgcggccgag gcgttcgcca accggctcat cgcgctcgag
     7921 cgcgaggtcg aggggctcaa gacgctcgcc ctccagggcg cccaggcgtc cgatcaggcc
     7981 aaggccgccg tgcagcagaa cagcgctgcg ctgcagaaga agctcgccga gaagcagaag
     8041 ctgctgagcc agctcgacca ggccaagatg caggagcagc tcaacaaggc gctcggttcg
     8101 ctgtccgagg tcgtcggtca ggacgtcccg acgttcgacg agatcagccg caagatcgag
     8161 tcccgccagg ccaaggccat ggccggcgcc gagctcgagg gcagcagcgt cgaggcgaag
     8221 atgctcgagg tcgagcaggc gtcgatcaac gtcgaggcgc aggcccgcct cgaggagatc
     8281 cgttcgcaga tggggctgtc caccgctccg gccgatgccc ccgaggccgc tccggcgacc
     8341 gagccggcca ccggtggcga agccggcgcc gccggctgag cgcagccggg tgaacgcacc
     8401 cgactgaatc actcggcccg gcggtgcgga agctcggctg aaccaccgcc cgtcaggaca
     8461 ggtcgaggat gatcaggtcg tccttgtgca cggcgacgcc acgaccgacg ccgccgtcga
     8521 tctgcgaggc gtcgaggcga acggtgccct ttccgcacac ggtgccgtcc ggaccgacga
     8581 tctcgaccgc gtcaccgcgg tggaacgagc cctcgacctt gcggatcccg ggggcgagga
     8641 gcgacttgtc gctcaggagc gcttgcaccg ccccggcatc gacatgcagg cggccccggg
     8701 ctccgaccgc gaacgcgatc cacaccttgc gcgccgacag gcgtcggccg gccggccgga
     8761 agatcgtgcc gacaccctga accccgtcga tggcgtcgat gagcacatcg ggccggctgg
     8821 ccttggcgat cacggtgcgc acgccgctcc aggtcgcgat cttggcagcc gccagcttgg
     8881 tggccatgcc gccgctcccc cggttggaac cgctcccgcc ggccagcgac tcaagctcct
     8941 gatcgacctc gatcacgtcc tcgatgagtg aagcgtgctc gtcgacgctc gggtcggccg
     9001 tgagcaggcc gggagcgtca gtgagcagca cgagcagatc ggcccgaatg aggtgcgcca
     9061 cgagggcggc gatgcggtcg ttgtcgccga tgcggatctc gtcgtcggcg atggcatcgt
     9121 tctcgttgat gaccggcacc acgcccagct cgccgaggcg acccaaggtt gcgcgggcct
     9181 ggagataggt cgaacgtcgg ttgaggtcgt cggggacgag caacacctgg ccggccacga
     9241 cgtcgtgttc ggcgaagcac tcgttccacg tcgccatgag gtgggcctgg cccaccgccg
     9301 acacggcctg cagcgtccgg ggatccttgg gccgctcacg ggcggtgagc ccgagcgcgg
     9361 gaagcccggc agcgatcgcg cccgaggaca ccacgaccac ctcgtgaccg gcctggcggg
     9421 accggacgac gtcgccggca accttcgcga tggccgagcg atcgatgcgg ccgtcgacgt
     9481 cggtgatcga cgacgtgccg accttgacca ccacgatcat gacggccctc gactcccctg
     9541 cccctgcgcc gccgtcattg cttccggcct cggtcctccg cctcgtcggc gaagttgatc
     9601 tcgtcagcgt tctcgtactc gaagctgagc ttgccgatgt gcacgacgtc gccgttgcga
     9661 gctccggctc gggcgagggc ccggtcgacg ccgagcgagc tgagtcgctc gtggacgtag
     9721 tcgagcgcct cggcgttggt gaggtcggag accccgacgg cgcgcagcgc ctcgcgaccg
     9781 tgcacctcga actcgccgga ctcgtcgcgt tcgatgcgga tgccctcggg cgtcggacgg
     9841 tgcacgacgt aggcgtcggg catcgcggtc gcggcacggg cctcctcgac ggcgacgcgg
     9901 agccggccga tgagctcggg gatgccgagc ccggtgaccg acgagacgat gtggccgttg
     9961 aactcgagca gcgtgtcggg cgtcgccagg tcggcgcggg ccccgattcg gacccgctcc
    10021 cgctcgatga gctcgggccg gtagcgctcg agttcgtcga gcagcacggc ttcctgttcg
    10081 agcggcgtct gttcggcggt ggacgagagg tcgaggatga gcagcaggac gcgggcacgc
    10141 tcgatgtggc gcaggaactg gtgaccgagg cccttgccct cggcagcgcc ctcgatgaga
    10201 ccggggatgt cggcgagcac catctcgaag ccgtcgtcgg tccgcacgac gccgagattg
    10261 ggcacgagcg tcgtgaacgg gtagtcggcg atcttcggct tcgctgccga cacgcgggag
    10321 atgagcgtcg acttgccgac gttggggagc ccgacgatgg cgacgtcggc catcagcttg
    10381 agctccagcc acatccaacg ctcctcaccg acctcgccct gttcggcgaa cgacggggcg
    10441 cgacgacggc tggacatgaa gcgcgtgttg cccttgccgc cctgtccgcc gtgggcggcc
    10501 aaccagcggt caccctgctg tacgaggtcg gcgaggacgg tgccgtcacg gtccttgacg
    10561 atcgtgccct ccggcaccgg caccatgagg tccttgccgg tcgacccgtg cttcttcgca
    10621 cccgacccgt gggtcccgcc cgttgccttg cggtgcgggt ggtcacggaa cgcgagcaac
    10681 gacgccacgt tccggtcggc cacgagccag acgtcgccgc cggagccgcc atcaccgccg
    10741 tcggggccac ccttgggggt gtgggcctcc cgccggaagg acacacagcc ggctccgccg
    10801 tcgccgccct tcacattgag gccggcctcg tcgacaaagt tggacacgac gacagtgtag
    10861 gtgcctgtcc gcgagccctc cgcaggggtt ccatgacccg gatcaggcca aggtcgacga
    10921 agtcgggcct tggccccaat aggactgtgc tgtcggcgaa gccgacggat ctgacaccca
    10981 gcggacgaga cgaagcacgc gggccatcgg ttcagcgcga gtgcacatat cgaactgagc
    11041 gctctgcgct cagttcgaga tgtgcaccgg gtcaccgatg atgcggaccg accaggtcga
    11101 gcggtcggtc accgcgctcg tgaagacata gttgatcttc caccatctcg cggcgtacgc
    11161 cgtggcgtag ttggccggga ggtcgacggt gaggatcacg aatcgctcgt tgaacttgca
    11221 gttcgatgca cggttcggcc cgatctgggt catcgaggcg ttgcacccac tgacgtcgag
    11281 ctgggttccc gaacccgtca gaccgctggc accgtcggcc gtgcgatacg agaagttcac
    11341 ggggaagccg ttcggatcca ggatctggat gtagttgccg ccctcaccgg ggtcgaagag
    11401 ggtgatctcc atctgcttgc cggcgtggat cgggttgatc tcggcaaggt agaagttcgc
    11461 gttggacgag gtcgaggtcg cgaggaccga gatccagttc cggccgacga ccgtcgggca
    11521 cgtcgcgtca gttcgggagt cgcacgcggt gccggtgccg ttgtaggagg ccatgaccgc
    11581 gtactggttg gagccgttcg agccgagttc gccctgcagc gtccggacgc cgagcacgta
    11641 gttaccgccg acggccgatg tcgggatggt gcagaacgcc gaccatccgc tcacgcccag
    11701 gatcgtggcg tcgttgtcga cctcgttggg cgcgtaggcg cgggggttgc tctgaacgat
    11761 cggggtgcac tgcgtgatcg gattgtcgtc gtcgtcgaag ggtgtgttgt cgggcgatct
    11821 caacgtgaac gtggtggtca tgttcgttcc gtcgttgttg tccggactgc tcgtcgtctt
    11881 cgccgggctg tacaggtgga cgacgatcgg gagggcgcgg accgccggca ccgagatgat
    11941 gtactcgtac tggctcgtcg ggttcgcccg gtactgaccg ttggcggtcg agcccgggca
    12001 cacctgtccg gtcgccggcc agttgccctc gaagccgacg gcgaaggggt cgccctgctc
    12061 cttgggcgcg caccacccgt tgatcgaggc ccacatgcct tcgggctcgc tcgtgtacag
    12121 gttgccggtc ccgaagaagt tgcgggggct gccgagcgga accggcagca cgtacgtcgc
    12181 gatcgactcc cgcttgatcg tcatggacga catgaagaac cgtgagaaga agatcggcgc
    12241 gtcggtgtcg gtgatgctga cgcggagatc ggtgttgctg atctggccga cgacgatcga
    12301 gatgttggcc ttggacgagt cgaacccgtt ctgacgggcc gtctcgttcg cgaccgcggt
    12361 cgccttcgac aggtcgggca gccagacgac cccggccaac gccgcagcgt cggcggcctt
    12421 ctgcatcttg gaaccctcgg cgtaccagcc gccgatgtcg gtggccatgc cggagatcgc
    12481 gagcaacggc accatgatga tcgccgacac cgcgaggacg tagcccttct cgtcacgacg
    12541 acggcgccgc cccaggggca gcgacggcag gaagcggcgg gcgcggcggg gtatcacggg
    12601 ttccccgcgt tgggctcgat gcgcatgatg gcgcggtcct tgacgaccat cgtcgacccg
    12661 aacgccttgg tcttggtggt gtggttgatc cgcacgtaca cacccaggta gtcggggccg
    12721 gtcgacgcgg cctggctgtt gccgcgtgtg gacgggcacc agaatcggtc ccggctgctc
    12781 ccgcagttgc ccgtgaacgt ggtggaggtc gacgcagcga tcgcctcggc gccggtgtac
    12841 acgttgcact gcgacgcgga gctgcccccg gtcgcccgag tcgagccggt caggcagtcg
    12901 gcgggcacgt caccgtcggc cgtggtggca cggtagatga cgatgacgtc gatctcggag
    12961 accgggacgg tgccgagcga agctgcaatg gaggcgaggg ccgacttgtc ggcggtcgcc
    13021 gccacgccca ggttcgaggc gacgcgggcg ccggcgcggg cggcgttggt caccgtcatc
    13081 gacgccttcc accccagtcc gaggtcgaag acggccaacg agatcaacat caggaggggg
    13141 aggatcagga cgacctcgac gagcacggcc ccgttctcgc cgcgggcgcg ccgagaacgg
    13201 cagctccccc tgggcgcggc gctgctcatg cgcgcaccgg ttcgagccgc atgacgaact
    13261 cgtcgctgaa gtcgcgggtc atgccgatca cgccggtgac gccggtgtgc cgggtctcga
    13321 tgtacacgcc gatgtagtcg ggcggatcgg agaccgcgac ctttcgcccg gccgggcacc
    13381 agaaccggtc gggcgacgtg tagttgcagc cgtagttcgc cgagttggtc gagatgctgc
    13441 cggcgatgta ggtgttgcag aggccggtga cggatccggt ccggcacgcc gcgagcgcgc
    13501 ccgacgacgt ggtcgacgtg ggaccggtcg ccttgaagat gatgatcttc ttgatggcgc
    13561 ccgtcttgaa gcccgacaga ttgccggtga tctcgttcac gatctggaag tccgcgtcgg
    13621 cgtccttgcc cagggtggag gcgaatctcg cggcgtcgcg ggtcgcattg gcgagcgtca
    13681 ggtagctccg gaacgccaga ccgaactcca tcgtcccgaa gatgaggagg aggaacaggg
    13741 gcaacgcgag cgcggattcg accacaacgg cgccgcgctc ggagcgcgtg agggagcgtt
    13801 cgtgtcggaa ccgcaacgct tcgcctcccg tcatctcgac aaccctgacc tcctcatatc
    13861 gggtcctgta ccccggttct tgagccggac cgcccaaggg aacactcagg aagctcagcg
    13921 gtccgggact gtcggtggtc gctcgtagca tgagggcatg tcgaagtcag ccgttcccca
    13981 cgacaccgac ccggtcgccg agccccgccc cgtcgggtca cgccgcgccg agcagtgggc
    14041 ggcgacgatg gccggccatg gccaacccgc aggcggcgag gcgccggtgg cgctgcgcca
    14101 cgctcatcgc gaagggttca acaccatcgg aacgcgcccc atcgccgacc gtcaggcccg
    14161 cgaggagctc gagcgggcga cgctcaccga gggggccacg ctcgccgtcg gcgccggcac
    14221 ccgggccagg cccgaggcac ccgatccgct ccgcacctgc ttcgaacgcg accgcgatcg
    14281 catcctccac ggcaccacgg cgttccggcg gctcgccggc aagacccagg tgttcatctt
    14341 ccccgaggat catcaacgca cccggctcac ccacgccctc gaggtcgccc aggtcgccac
    14401 cgccattgcc ggggcggtgg ggctcaacgt cgtcctcacc gaggcgatcg cgctcggcca
    14461 cgactgcggg cacggcccgg gtggccacgc gtcggaagac gcattcagtc catatgttcc
    14521 cggcgggtac gaccacgcgg tctggggggc cgacgtggtg ctcgagccgc tcaacctctg
    14581 cgtcgagaca ctcgacggca tccgcaacca cagctggtcg cgtccgacgc ccctcacacc
    14641 cgaaggtgag gtcgtctcgt gggcagaccg catcgcctac gtctgccacg acttcgagga
    14701 tgccgtgcac gccggcatcg tcagccccga catgctgccc gaggtggtcc gtgaccggtg
    14761 cggggccacg cgctccgagc aactcggctc gttcatcagg gccatggtcg agaccatcgc
    14821 gcgcaccggc cacatcgcgc tcgacgagcc caccgccgac gcactcgccg cgttccgcag
    14881 gttcaactac gagcgcatct acatgcggcc ggcctcggtg gcccagagcg agtccgtcat
    14941 ccgggtgctg cgcgcgctcg tcgagtacta cgccgatcgc cccaacacgc tgttgctcga
    15001 cgacccccac caccgtccgg cccaccagcc ggcggtggtg cgcccccacg ccgccgccgg
    15061 cagtcccgag gcgctgcacg acgcggtgtc gtacgtggcc ggtatgaccg atcggttcgc
    15121 gttcaacgcg gccatcggcc tccttggatg ggaccgcgac acgctccccc aaggcgtcgg
    15181 catccgctga gtatcagccc aaaactgcca cgagggccac gccggagcgt gaccctcgtg
    15241 ggagaactgg tgtcgatcta gtcggtgagg acgtcgacga gcttcttgcc cttgcgggac
    15301 ccgaacttga ccttgccagg agcaagggcg aaaagcgtgt cgtcgccgcc acggccgacg
    15361 ttgacgccgg ggtagaactt ggtgccgcgc tgacggacga tgatggagcc ggcgttgacg
    15421 acgccgccgt cgaacacctt cacgccgagg cgcttggact cggaatcgcg accgttcttg
    15481 gttgagccgc cgcccttggt ctttgacatg tcaggtcagc ccttcgtgat gccggtgatc
    15541 tcgatggtgg ccaacgtctg acgatgaccc cagcggcgcc ggttgttcga cttgttcttg
    15601 taggtgaacc caatgatctt gggccccttg gtctcctcga cgatgcgagc cgagacggct
    15661 gcgcccttga gttgggcagg agtggcgagg gtgttgtcgc cgtcgacgag cagcaccggc
    15721 gtcagcgaga cgtccgtgcc gggctcaccg agccgctcga cctggacgcg atcgccctcg
    15781 gccacgcggt attgctttcc accggtcttg atgactgcat acatagcgtc ggcaacgtta
    15841 ccaggtcggt ccgtcactca gcgaaccgac gccgccgtgg ccatcaagtc agtcgatgcc
    15901 gacgacggcg ctggtcagca cgatgccacg gccgttgcag accgagcact ggtcggcgaa
    15961 ggactcgagg agcccctccc cgatgcgctt gcgggtcatc tcgacgagac ccaggtcgct
    16021 gacggagaac acctgcgtcc gcgtcttgtc gcgggccagc gcctccttga aggtacgcac
    16081 cagttcgtcg cggttggcct tgatctccat gtcgacgaag tcgatcacga tgatgccgcc
    16141 gatgtcgcgg agccgcagct gccgggcgat ctcgaccgct gcctcgaggt tgttgcggta
    16201 cacggtctcc tcgagcgagg aggaaccgac gttgcggccg gtgttgacgt caatgacggt
    16261 cagcgcctcg gtgtgctcga tgatcagcga cccacccgac ggcaaccaga ccttgcggtc
    16321 gaggcccttc ttgagctgtt cggtgatctg gaaccgttcg aacagcgaga ggttctcggt
    16381 ctcgaggtcg tagtgctgca cccgatccgc cgtcgcgggc gagatggccg cgacatagtc
    16441 gtgcacctcg gagtacaacc cgggctcgtc gatgatgacc gcgcggtact cctcgttgaa
    16501 ctcctcacgg atcacgcgga ccgccatgtc gggctcgcgg tacaggagcg ccggccggtt
    16561 ctgcttggcg gcaagcgccg agatctggtc ccactgcttg agcaggcggg tcacgtcacg
    16621 ttcgatttcc tcgctcgtca cgttctcggc ggcggtccgc acgatcacgc cgtggtgggc
    16681 gggcttcacg cggtcgagga tctggcggag ccgcttgcgt tcctggtccg acagccgctt
    16741 ggagatgccg taggtcttgg agttggggac gaggaccacg aatcgaccgg ggagggagac
    16801 ctcctgggtc aaccgggcgc ccttgtgccc gatggggttc ttggtcacct ggcagaggat
    16861 cagctgcttc ggcttgagga cgtcctcgat gcgggcatcg gccttgacct cgacatcgtc
    16921 ggcgtcgagg tggacgtcgc cgcggtagag caccgcgttc ttgggcgtcg cgatgtcgat
    16981 gaacgccgct tccatgccgg ggagcacgtt ctcgacccga ccgagataga tgttgccgtg
    17041 gatctggctg acgtcgtcgg aggggcgcga cacgtagtgc tcgatgagcg accggccctc
    17101 gaggacggcg atatgggtgt gtcccgccga gtcgacgtgc acgttcatct ggtagcgccc
    17161 gaccgcccgg ccgttgcgct gccggccctg ccgcttcttg agcgccttcg cgtcgagatg
    17221 aaccggcccg tcgtcgtcgg tcaacacggc ctcgatcggc gtggccgccg ggcgcttctg
    17281 cccgccgcgc tggccaccac caccaccgtt cctgccgcca ccctggcctt ggccctggcc
    17341 gccgcgggcc tgacctccac cacgctgacc gccctggcca ccgccttggc caccgtcctg
    17401 ggggctgcgt ccgccgttgt cacggccctc gccgccaccg ccaccgccac ctcggctgcg
    17461 gcgacgacgc cgcttcgccg aggtgctcag gccctcaccg ggcgccgacg gctgaccgcc
    17521 cgccgcggcc ggcgcacccc cggggatcga atcgccgatc tggggtttgc gtggtgccgg
    17581 cggcgggacc ttcggcacca gcggggccga cgtgggtggt gtggtgggct ctgtctcgga
    17641 catgtgggcg tacctctcag gaggccagtc gttcgccgac cggctgcgac gggtcaccgg
    17701 ccaccagggg cggcagcgac gttccgtcga tcgtcatcca ttgttcgttt cggcagatac
    17761 gacgcacctc gcccgcctgc tcgaggcagg tcaggaactc ggaggggcgg atgctgcggg
    17821 gcttggtcgc gaggtcgacg gtgagcacca ccgcgccctc tcggagtgct ggctgggtag
    17881 tggccgagag cagcgctggg cg
//
LOCUS       DAOSOA010000020        17843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3190_length_17843_cov_6.451934, whole genome shotgun sequence.
ACCESSION   DAOSOA010000020 DAOSOA010000000
VERSION     DAOSOA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17843
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3190_length_17843_cov_6.451934"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKY13_02755"
     CDS             complement(<1..467)
                     /locus_tag="PKY13_02755"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT78875.1"
                     /translation="MAVLSPNLAGSLRAAADAAIEVAVPVSFSRLGFSVRRSLWGWGP
                     STAAAAGRTIVVTGATSGLGYETAQELLAAGANVEIVARDRARGAAAARRLGAAAGVD
                     PPVVRLADLGDLDAVRALADELRTAHDTIDGLVHNAGAMPTRRVVTGDGLESA"
     gene            complement(546..1295)
                     /locus_tag="PKY13_02760"
     CDS             complement(546..1295)
                     /locus_tag="PKY13_02760"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOT78876.1"
                     /translation="MSNVDEWDGKVPGAFNLRDLGGNPVAGGTIAPGRVFRCDLLHRV
                     DAELGARWLHQNDVRTLIDLRTLGEREGDGFMAASEHLDSLHRGLLDEVWSWEAENAA
                     EDDWFLRDRTLEMYERFPDRIAQVLGDIAAAPANVLFHCTAGKDRTGVIASALLGVLG
                     ATPEVIAADYARSTQAMAGIVGWYRSLAGDIPHDPEREQQIVDRAATVATMAGVVEEI
                     ERRHGSFAGWARDAGIGDDLIEALRAKLVVS"
     gene            complement(1292..2452)
                     /locus_tag="PKY13_02765"
     CDS             complement(1292..2452)
                     /locus_tag="PKY13_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78877.1"
                     /translation="MAHRSTDDGSAPEPLDHHWALSAVVSEEFLNTLARNGIGDGIEA
                     AELRQQFNLPLLGSIDLSIGMVIVEVSFQMLEEHGDRLLATIRATGAVTFHGDTLMPP
                     LPGLAHVRGDVLVRPRVELHPDGRFIAILDLAGSELVGMTFEGIDGIEADAEAQAMMG
                     QMLFAAVGGELFEGLADQMGSVGLELEPEQGHVFRELGVHPAPADIEVHQGAMTVGLP
                     AVPGLEGRADAERVSGHRVGVGVAAGALSALANRLAADAIGTPLPFEFDVIARDDRVG
                     ARVRNQRLVDSPYLPDLRPGFRSTVRPRLVGGNLELSLREAWVELPLVPAAVNRLNRF
                     FGGVASLAPLNVSIPAHTTIPVRPDSDATLAMSIVGLEVERDGVAFVVEAHL"
     gene            complement(2462..3352)
                     /locus_tag="PKY13_02770"
     CDS             complement(2462..3352)
                     /locus_tag="PKY13_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT78878.1"
                     /translation="MAQYLEHIGAYELLGPERERLLAAEIAEGRAASARLADGVASRA
                     ERTRLVAAVRAGEAAFDQFVNANLRLVVSVAKRYQATGVPLLDLVQEGNLGLIRAVEK
                     FDHTKGFRFSTYGVWWIRQFISRGIASSRSTVRLPTRANDDLQRLRLAVGRSELESGR
                     TGTTAELAELAAMSDERVVELLPMLADPVSLSSAVGDGGAELGDFIEDAAALGEVESA
                     ADRLRPEEIDRLLSPLDPRERVIIELRYGFDGGEGCSMGEISRRLGLSRERVRQLEQR
                     AQAKLSHPSRPPLREFIRGC"
     gene            3519..4520
                     /locus_tag="PKY13_02775"
     CDS             3519..4520
                     /locus_tag="PKY13_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HOT78879.1"
                     /translation="MTEPTDPSRHDDAPLDDEAAALTELDHDPHTRAEIRHARRAFLM
                     EAAIEAERDTGRREQTDEEVRHSLWMRVARLLGGWILVLLGLAAIPLPGPGWLMVLLG
                     LTLLPYRWTDNLIREIRRRIPGIPEDGRIPTRTWIITGVLVVGATAGSVWWGMRDQGD
                     DEPAKGDRVEEAAGASTADDDRDDGGAGPGPTIVVASSAQPERVQLAQIYGEVAATVT
                     DGVSDHIDRDDTCAALTSGDADLVLLDENELSACFGGETDQAARLRDAGVKMYAPVVA
                     GATTFFVTVRSDAIAIDDEPAELTLDGVSSQISAGQDVAGDPADRARELMAAAGVLK"
     gene            4525..5502
                     /locus_tag="PKY13_02780"
     CDS             4525..5502
                     /locus_tag="PKY13_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT78880.1"
                     /translation="MTFPEPQLITLTDPDYGRIELSVHEQGTGPAVVFSHGFPELAYS
                     WRHQLPAVAAAGFRAIAPDQRGYGGSTAPEPVAAYGMDQLTGDLVRLLDELGLDQAVF
                     VGHDWGGFVVWAMGVLHPERCAGIVGVCTPYLPFPGTDFLKMLVDGDVERQYMLWFQE
                     PGVAEREMDPRARVLFEKLMVGGVDPRIIAERAMADGKLNMNPFIDLDGLEPLGESIA
                     DADVVDHYASVFERTGFRGGINWYRNVDANSAAHPEVGTTVLSMPTLMLCAEWDPALP
                     PALAAGMPNVCSDFETHTIPKAGHWVQQESPDAVNELLLDWLQRRFGSA"
     gene            complement(5512..6945)
                     /locus_tag="PKY13_02785"
     CDS             complement(5512..6945)
                     /locus_tag="PKY13_02785"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HOT78881.1"
                     /translation="MATQVQTLNYIGGEFRAARSGAVDEVLDPATGEVIAEVASSDAA
                     DAADAVEAASAAFESWRKTTPRERFEMITKLADAIEADLPELKRLESLNVGKPVSIID
                     FEFDLTVDNFRFFAAAARFMEGRAGGEYLEDHTSFVRRDPIGVIVGIAPWNYPLNMAT
                     WKIGPAIAAGNTMVLKPSELTPLTALRLAELSADILPPGVLNVVSGRGATAGSALVDH
                     PRVDMISLTGGVATGKAIAQAASASLKRLHLELGGKAPVVVFDDADIELLVTTMTDMA
                     YYNSGQDCTAPCRIVAGPRVFDDIVSGLTESVAALRTGDPFDPQTNVGPIVSQAHQQR
                     VAAMIGRAAEAGADITTGGAIIDGPGFFVEPAVIVNPAQDSEIVQRELFGPAVSVQRF
                     SDEEQAVAWANDCDYGLASSVWTTDVGRAMRVSRDLHFGTVWVNDHIPIVSEMPHGGY
                     KQSGYGKDMSIYALEHYTELKHVMVKH"
     gene            7136..8059
                     /locus_tag="PKY13_02790"
     CDS             7136..8059
                     /locus_tag="PKY13_02790"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOT78882.1"
                     /translation="MSPPDLQVARLAARQHHCFARCQALEIGLSYRQIRHRVDVGRWT
                     QLHDSVFCLAGAPIAALGHIHAAVLALPGSAASHGSALHLWGERDPAPRVAHVIRRHG
                     RSHRLHGVHLHQTRLLPQHHVTVAQGVPVTTRARTVFDLAATTDLATLGRLIDRQVVA
                     GSLRLDELELMLDEMVCPGRPGVGQFAAVLDQRVGHGPGVSMLEQRYRSLCERRSLPL
                     GDPEARAPWATSGGPRSERVDFAHGRERVIVELDGRTYHARLEDFENDRRRDHLALAA
                     GWRTVRFTWRQVTTDPDHVERILRSVLDVGA"
     gene            complement(8141..9016)
                     /locus_tag="PKY13_02795"
     CDS             complement(8141..9016)
                     /locus_tag="PKY13_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOT78883.1"
                     /translation="MAEIITPRRSVLYMPGANDKALEKAKTLPCDAIIFDTEDSVAPD
                     MKAVAREKVAAAVRSGEYGRRELTIRINGLDTEWWKADLESAGAAAPSAVVVPKINSA
                     ADVALVEGVLDGIDAAANTTIWAMLETPAAMERAVEIATSSDRLTVLIMGTNDLAKEL
                     RAALVPGRAPLLWGLGRCLNAARFAGKVILDGVYNDVKNPEGFAAECAQGAEMGFDGK
                     TLIHPTQIEPCNDAFAPSAAEVEHSERVIAAWDEGIAAGKGVITVDGRMIENLHVDNA
                     RRALAIAAAIADLES"
     gene            complement(9018..10085)
                     /locus_tag="PKY13_02800"
     CDS             complement(9018..10085)
                     /locus_tag="PKY13_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOT78884.1"
                     /translation="MRSSKDFFKPLAAGAPQPVTEIPFPPSRMIHFFPPHLEKVTAKL
                     PDIAPTVDILLGNLEDAIPADQKENARAGLVKVGKEMDLGDTQLWTRVNSLDSPWVLD
                     DLVTLVGEIGDKLDVIMIPKVEGPEDIHYVDRLLAQLEAKAGLTKPLLVHAILETARG
                     VANVEEICLASPRMQGLSLGPADLAANRRMKTTRVGGGHPGYLVREDPAQTADGPDYQ
                     GARTSYQQDLWHYTIARMVDACATAGILPFYGPFGDIKDTVACEDQFRNAFLLGCVGA
                     WSLHPVQIDIAKKVFSPDPKDVAHALHVIEEMGDGTGAVMIDGKMEDDASVKQCHVMV
                     NLAKMLSARDPELAATYGFGG"
     gene            complement(10115..11524)
                     /locus_tag="PKY13_02805"
     CDS             complement(10115..11524)
                     /locus_tag="PKY13_02805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT78885.1"
                     /translation="MSSASTRAHRALVDARPEVLWLDTPDRPDPGRTLCGGTRADLVI
                     VGAGFTGLWAAIQAKEDDPSRTVVVVEAGRVSEQASGRNGGFCSSSLTHGLDNGVARF
                     PSEMARIEELATENFAGIAATIERYGIDCDWQPTGGLTVATAPWLVDDLHEQVELTRR
                     FGHDVEFLDRDAIRDRVASPTYEAGVVQRTGEAIVDPARLGWGLADAARSLGVEIYER
                     TPMLGLARSGAGMAVRTESGTIHCGAVVLGTNAFKSPIGAINRTIAPVYDYVLATEPL
                     SADQLASIGWSGREGISDTTNQFHYYRLTADNRILWGGYDAIYHFGSKIDPSLDQRDE
                     THQVLAEHFFETFPQLEGLSFSHRWGGVIDTCTRFSVTFGTSFDGRVAYAVGYTGLGV
                     GATRFGARVCLDLLFHPDSELLSLRMVKRKAIPFPPEPVRWAGIELTRKALAKADANE
                     GRRGPWLRLLDSLGLGFDS"
     gene            complement(11640..11819)
                     /locus_tag="PKY13_02810"
     CDS             complement(11640..11819)
                     /locus_tag="PKY13_02810"
                     /inference="COORDINATES: protein motif:HMM:NF025379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HOT78886.1"
                     /translation="MDLGDITDKAKGLLEEHADKVDEGIDKAAELADDKTGGKYSDQI
                     DTAADKLKDVLPGGS"
     gene            11964..14438
                     /locus_tag="PKY13_02815"
     CDS             11964..14438
                     /locus_tag="PKY13_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOT78887.1"
                     /translation="MTERPPGMYVGELTDAATHERAGTPLGLNPDDFTTHGVIVGMTG
                     SGKTGLGIVLLEEALTAGVPTLILDPKGDMGNLLLTFPNLAPDDFAPWVPAGQDAAGV
                     ADTWTKGLADWSVAPTEIATLRTGHRMSVYTPGSTSGIPLNIIGSLAPPTNSTGGTPD
                     LEAIHDEVESLVQGLLGLVGVTSDPLSGREHVLMANIIELAWAAGETLDLATLLIRIQ
                     DPPLRKLGVIDVETFFPKPDRTALMMKLNGLLASPSFSAWGHGAPIDIESLLWSDDGT
                     ANAAVIYLAHLSDEERQMVVTRVLSKLVGWMRGQPGSTKLRVLVYMDEVYGFVPPTAM
                     PPAKKPILTLFKQARAFGVGVVLATQNPVDLDYKAISNAGTWMIGRLQTERDKARLLD
                     GMSSAAGTVDLATVDATISSLAKREFLLHTTGGKPPRTFGVRWAMSYLAGPISKDQVP
                     RLPGQASAPAPEDAGDASSPRYREGAPTAPSTPSSAPVDAAEPPQDGQADTAGSSGAG
                     GLAHDETPAMPAVAAGVPVRFLDPAAPWAPTLGAVSGGRRLQACVAVRVNLLFDDTPS
                     GLRHTEQIEAIFAPLDQPRIDPAKAYSVDYDDRDLQTSAPAGAVFVLPSAPVNTKAYF
                     NDVESSVKDYLFRSRHTTIYGNKSLKLYSRPGESQADFTARCHAAADAGADRDTDQIR
                     QKLQLKIDRINEGIAADRQRAQQLEEEAAASRRNEAVSIGGSVLGAIFGGRKSARSIS
                     AAANRAATGRARSQKADNRMEQALARIDEKMANIDDLQHQLADEITDIVAHWSGLAAD
                     VVTLEIPLEKSDIGIEQVALVWVPTA"
     gene            complement(14455..15492)
                     /locus_tag="PKY13_02820"
     CDS             complement(14455..15492)
                     /locus_tag="PKY13_02820"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOT78888.1"
                     /translation="MTEPIPPSAPSPSAGNSPVLEALDVVKEFPVTGGLFGRTIGQVH
                     AVSGVSMSVDRGETLGIVGESGCGKSTFGRVVLRLLTATAGTVKLGGQDTAKMPNKEL
                     RRRAQMVFQDPYASLNPRMTIGEVLAEPFLVHQGKKAKECTEEVSELLRTVGLSPEHR
                     SRYPHEFSGGQRQRIGIARALAVDPEVIVLDEPVSALDVSIQAGVVNLLERLQDERGI
                     AYLFIAHDLSVVRHISDRVAVMYLGKLVEVGTKAQIYTQPRHPYTNALLSAVPIPDPR
                     KERARKRIVLTGEVPSPIDPPSGCRFRTRCWKATDLCATTEPPLVPVDGDATDHRVAC
                     HFPELGDRPPL"
     gene            complement(15495..16544)
                     /locus_tag="PKY13_02825"
     CDS             complement(15495..16544)
                     /locus_tag="PKY13_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78889.1"
                     /translation="MGRLLEISDLHVGFRTEDGVVQAVRGVDMSIDDGDVLAVVGESG
                     SGKSVTALSVLGLHPEGRTNMTGRIEWEGENLIGAKGDRLRQVRGGEIAMIFQDPLSA
                     LNPVYTVGKQIIEMLQSHRQISKADAKNRAIEMLELVGIPQPQRRVDNFPHEFSGGMR
                     QRAMIAMALSCDPKLVIADEPTTALDVTVQAQVLELLVSVSRQQGAAVMLITHDLGVV
                     AGLANKVAVMYAGKVVEQASTDELFDDPTHPYTIGLLRSLPRVDADGDDQLVPIGGQP
                     PSMLNPPAGCAFHPRCAIARSESGCDSMMPTLFDETPAAIVSGTTGPAVDRSGHGAAC
                     WRRDESRALLGVVGA"
     gene            complement(16546..17487)
                     /locus_tag="PKY13_02830"
     CDS             complement(16546..17487)
                     /locus_tag="PKY13_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT78890.1"
                     /translation="MTDPRLTPEVPAQQPVADTPIGVVALDATLADVGPSRSLFEDAW
                     RRFRRNKLAMFGLFLVIFLVLVAIIGPFVVQSPTKLSKTVILEPPTNQHLFGVDNKGA
                     DIFARVVHGIRLSLFIGVMATLLETFIGIAVGALAGWYGKWVDSVAMRFVDIMLGIPY
                     IILAYAFITVFGKGVPAVIFALAFTAWLQTARTVRAGFIQVKEFEYIEAARALGVSGP
                     RIMLRHILPNVLQPVVVLMAVGVGSAILSEAALSFLGVGVKPPNPSLGLMVSDAQSFL
                     TSAPYMFLFPGLAIVLTVLGFLLVGDGLRDALDVKDN"
     gene            complement(17484..>17843)
                     /locus_tag="PKY13_02835"
     CDS             complement(17484..>17843)
                     /locus_tag="PKY13_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT78891.1"
                     /translation="EDLLEREWALSADHVRTARAKGLTEKAISRRHVLRNALIPVVTF
                     IGIDFGTLIGFAILTETVFQWPGLGSKVARAAGQSDLPVVLTLSMVVMLMYGLANLAV
                     DISYAKLDPRIRLSGAK"
BASE COUNT         2772 a         6198 c         6168 g         2705 t
ORIGIN      
        1 cacgccgact ccagtccgtc gccggtgacg acccgacggg tcggcatggc tccggcgttg
       61 tgcaccaggc cgtcgatcgt gtcgtgcgcc gtgcggagtt cgtcggcgag ggcccgcacc
      121 gcgtcgaggt cgcccaggtc cgcgagccgg acgaccggtg ggtcgacgcc ggccgccgca
      181 ccgagacgcc gcgctgctgc ggcaccccgg gcccggtcgc gggcgacgat ctcgacgttc
      241 gccccagcgg ccagcaactc ctgagcagtc tcgtagccga gcccactcgt ggcgccggtg
      301 acgacgatgg tgcgcccggc agccgcggcg gtcgacgggc cccagcccca cagcgatcgg
      361 cggacggaga atccgagccg ggagaagctg acgggaacgg cgacctcgat cgccgcgtcg
      421 gcggcggccc ggagcgagcc ggccaggttc ggggagagca cagccatggt gctggtctac
      481 cccgggccgc catcagcgcc cactcatccc acggccgggc gaggtcgacg gcggtcgcgc
      541 gcgacctacg agacgacgag tttcgcccgc agcgcttcga tgaggtcgtc gccgatgccg
      601 gcatcacgcg cccaccccgc gaacgagccg tgccggcgtt cgatctcctc gacgacgccc
      661 gccatcgtcg cgaccgttgc cgcccgatcg acgatctgct gctcgcgttc gggatcgtgg
      721 gggatgtcgc cggccagcga gcggtaccag ccgacgatgc cggccatggc ctgggtgctg
      781 cgtgcgtagt cggcggcgat cacctcgggc gtggcgccga gcactccgag cagggcgctc
      841 gcgatcacgc cggtgcggtc cttgcccgcc gtgcagtgga acaacacgtt ggcgggcgcc
      901 gcagcgatgt cgccgagcac ctgggcaatg cggtccggga agcgctcgta catctcgagg
      961 gtccggtcgc gcaggaacca gtcgtcctcc gcggcgttct cggcctccca gctccacacc
     1021 tcgtcgagca ggccacggtg caacgagtcg aggtgctcgc tcgccgccat gaatccgtcg
     1081 ccctcccgct cgccgagggt gcgcaggtcg atcaacgtcc gcacgtcgtt ctggtgcaac
     1141 cagcgggcac cgagctcggc atcgacccga tgcagcaggt cacagcggaa cacccggccg
     1201 ggggcgatgg tgccaccggc gacggggttg cccccgaggt cgcggaggtt gaacgctccg
     1261 gggaccttgc cgtcccactc gtcgacgttg ctcacaggtg tgcctccacg acgaacgcga
     1321 ccccgtcgcg ttcgacttcg agcccgacga tcgacatcgc gagcgtggcg tcgctgtcgg
     1381 gccggacggg gatggtggtg tgtgccggga tcgacacgtt gagcggcgcg agcgaggcga
     1441 cgccgccgaa gaagcggttg aggcgattga cggctgccgg caccagcggc agctcgaccc
     1501 acgcctcacg gaggctgagt tcgaggttgc cgcccaccag acgggggcgc accgtcgacc
     1561 ggaacccggg ccggagatcg gggaggtagg gcgagtccac cagccgctgg ttgcgcaccc
     1621 gcgccccgac ccggtcgtcg cgggcgatca catcgaactc gaagggcaac ggggtcccga
     1681 tggcgtccgc ggcgagccgg ttggcgaggg ccgacagcgc accggcggcg accccgacgc
     1741 cgacgcgatg gccgctcacc cgttcggcgt ccgcccgacc ctcgagcccc ggcaccgccg
     1801 gcagaccgac ggtcatcgca ccctggtgca cctcgatgtc ggcgggagcc gggtgcacac
     1861 ccaactcacg gaacacgtgc ccctgctcgg gctcgagctc gagcccgacc gagcccatct
     1921 ggtcggcgag accttcgaac agctcgccgc cgaccgcggc gaagagcatc tggcccatca
     1981 tcgcctgcgc ctcggcgtcg gcctcgatcc cgtcgatgcc ttcgaacgtc atcccgacca
     2041 gctcgctgcc ggcgagatcg aggatcgcga tgaagcgacc gtcgggatgc agctcgacgc
     2101 ggggacggac caggacatcg ccgcggacgt gggcgagacc gggaaggggc ggcatgagcg
     2161 tgtcgccgtg gaacgtgacg gcgccggtgg cccggatcgt cgccaggaga cggtcgccgt
     2221 gctcctccaa catctggaac gacacctcga cgatcaccat gccgatgctc aggtcgatgc
     2281 tgccgagaag ggggaggttg aactgctgac gaagctcggc cgcctcgatg ccgtcaccga
     2341 tgccgttgcg ggccagcgtg ttgaggaact cctccgaaac gacggccgac agggcccagt
     2401 ggtggtcgag aggttcgggt gcggatccgt cgtccgtgga gcggtgcgcc atggagcggt
     2461 tctagcaccc ccggatgaac tcgcggagcg gcgggcggct ggggtgggac agcttggcct
     2521 gtgcccgctg ctccaactga cgaacccgct cccgggacag gccgaggcgg cggctgatct
     2581 cgcccatgga acacccctcg cccccgtcga acccgtaccg cagttcgatg atgacccgtt
     2641 cccgcggatc gagcggtgac aggagccggt cgatctcctc gggacggagt cggtcggcgg
     2701 cggactcgac ctcgccgagc gcggcggcgt cctcgatgaa atccccgagc tcggcgccgc
     2761 cgtcacccac ggccgacgac aacgagaccg ggtccgcgag catcggcagc agctcgacga
     2821 cccgctcgtc gctcatcgcc gccagctcgg cgagctccgc cgtggtaccg gttcggcccg
     2881 actccaactc ggagcgaccg accgcgaggc ggagtcgctg caggtcgtcg ttcgcccgcg
     2941 tcgggaggcg caccgtcgac cggctcgacg cgatgccccg ggagatgaac tgtcggatcc
     3001 accacacgcc gtaggtcgag aaccggaacc ccttcgtgtg gtcgaacttc tccaccgccc
     3061 ggatgaggcc gaggttgccc tcctgcacca ggtcgagcag cggcacaccc gtcgcctggt
     3121 accgcttggc gaccgacacg acgagccgca ggttggcgtt cacgaactgg tcgaacgccg
     3181 cctcgcccgc gcgcacggcc gcgaccagac gcgtccgctc ggcgcgggac gccacaccgt
     3241 cggccagccg ggccgatgcc gcccgaccct cggcgatctc ggcggcgagc aaccgctcgc
     3301 gttcgggccc caggagctcg taggcaccga tgtgttcgag gtactgggcc actggatcga
     3361 gggatgttgc ggccgatgca gagggtcgcc gcgagaaacg ctggactgca accatgtctg
     3421 cggttcggag ccaaccggcc gttcggattc acccaatctg agaattcctt cacccggacc
     3481 gggtgctcct gccctgctgt tgtggccaca ctggtgcggt gaccgagccg accgatccct
     3541 cccgccacga tgacgcgcct ctcgacgacg aggcggcggc attgacggag ctcgaccacg
     3601 acccgcacac ccgcgccgag atccgccacg cccgccgcgc cttcctcatg gaggcggcca
     3661 tcgaggccga gcgtgacacc ggccggcggg aacagaccga cgaggaggtc cgccactcgc
     3721 tgtggatgcg ggtcgcccgc ctcctcggcg gctggatcct cgtgctgctg ggtctggccg
     3781 ccattccgtt gccgggcccg ggctggctca tggtgctcct cggtctgaca ctgctcccct
     3841 accgctggac ggacaacctc atccgcgaga tccgccggcg gattcccggc attcccgagg
     3901 acgggcggat ccccacccgc acctggatca tcaccggtgt gctcgtggtc ggcgccaccg
     3961 ccggttcggt ctggtggggg atgcgcgacc agggcgacga cgagcccgcc aagggagacc
     4021 gcgtcgagga ggcggcaggc gcctcgacgg cggacgacga ccgtgacgat ggtggggccg
     4081 gtcccggccc gacgatcgtg gtcgcgtcct cggcgcagcc cgagcgcgtc caactcgccc
     4141 agatctatgg cgaggtcgcc gcgaccgtca ccgacggtgt ctccgaccac atcgaccgcg
     4201 acgacacgtg cgccgccctg acctcgggtg acgccgatct ggtcctgctc gacgagaacg
     4261 agttgtcggc ctgcttcggc ggcgagaccg atcaggcggc ccgactgcgc gatgccgggg
     4321 tgaagatgta tgcgccggtc gtcgccgggg ccacgacctt cttcgtcacc gtccgttccg
     4381 acgccatcgc catcgacgac gagcctgccg aactgaccct cgacggcgtg agctcgcaga
     4441 tcagcgccgg ccaggacgtc gccggtgacc cggccgaccg cgctcgggag ctgatggccg
     4501 ccgccggcgt gctcaaatag cgcaatgacg ttcccggagc cccagctcat caccctcacc
     4561 gatcccgact acggccgcat cgagctctcg gtccacgaac aggggacggg gcccgccgtc
     4621 gtcttctccc acggatttcc cgagctggcc tactcctggc ggcaccagct cccggccgtc
     4681 gcggccgccg ggttccgggc gatcgcgccc gaccagcgtg gctacggcgg ttcgacggca
     4741 cccgaaccgg tcgcggccta cggcatggac cagctgaccg gcgacctcgt tcgtctgctc
     4801 gacgaactcg ggctcgatca ggcggtgttc gtcggccacg actggggcgg cttcgtcgtc
     4861 tgggccatgg gggtgctgca ccccgagcgg tgcgccggca tcgtcggtgt gtgcacgccg
     4921 taccttccgt ttcctgggac cgacttcctg aagatgctcg tcgacggcga cgtcgaacgg
     4981 cagtacatgc tgtggttcca ggagccaggt gtcgccgagc gcgagatgga tccccgcgcc
     5041 cgggtgctct tcgagaagtt gatggtcggc ggtgtcgacc cccggatcat cgccgagcgc
     5101 gccatggcgg acggcaagct caacatgaac ccgttcatcg atctcgacgg cctcgagccg
     5161 ctcggcgaat cgatcgccga cgccgacgtg gtcgaccact acgcgtcggt gttcgaacgc
     5221 accgggttcc gaggcggcat caactggtac cgcaacgtcg acgccaactc cgctgctcac
     5281 cccgaggtcg gcaccaccgt cctgtcgatg ccgacgctca tgctgtgcgc cgaatgggac
     5341 ccggcgctgc cgccggcgct cgccgccggc atgccgaacg tgtgctccga cttcgagacc
     5401 cacaccatcc cgaaggcggg gcactgggtg caacaggagt cgcccgacgc cgtcaacgaa
     5461 ctcctcctcg actggctcca gcgccggttc gggagcgcct gagcccgatg ctcagtgctt
     5521 caccatcacg tgcttgagct cggtgtagtg ctcgagggcg tagatcgaca tgtccttgcc
     5581 gtagcccgac tgcttgtacc cgccgtgggg catctccgag acgatcggga tgtggtcgtt
     5641 gacccagacc gtgccgaagt ggaggtcgcg ggagacccgc atcgcacggc cgacgtcggt
     5701 cgtccatacg gacgaggcga gcccgtagtc gcagtcgttc gcccaggcca cggcctgctc
     5761 ctcgtcggag aaccgctgca cgctcacggc cggaccgaag agttcgcgtt gcacgatctc
     5821 cgagtcctgg gcggggttga cgatcacggc cggctcgacg aagaaccccg gaccgtcgat
     5881 gatcgccccg ccggtcgtga tgtcggcgcc ggcttccgcc gcccgaccaa tcatggcggc
     5941 gacccgctgc tggtgcgcct ggctgacgat cggccccacg ttggtctggg gatcgaaagg
     6001 gtcgcccgtc cgcagcgcag ccaccgactc ggtgaggcct gagacgatgt cgtcgaagac
     6061 tcgggggccg gccacgatgc ggcagggcgc ggtgcagtcc tggccggagt tgtaatacgc
     6121 catgtcggtc atcgtggtga ccaacagctc gatgtcggcg tcgtcgaaca ccaccaccgg
     6181 cgccttgccg ccgagttcga ggtgcagtcg cttgaggctc gccgaggcgg cctgggcgat
     6241 ggccttgccc gtcgccacac cgccggtgag cgagatcatg tcgacgcgag gatggtcgac
     6301 gagcgcggag ccggcggttg cgcctcgtcc ggacacgacg ttgagtacgc cgggcgggag
     6361 gatgtcggcg gacagttcgg cgagacggag cgcggtgagc ggcgtcagct ccgagggctt
     6421 gagcaccatc gtgttccctg cagcgatcgc cgggccgatc ttccacgtcg ccatgttgag
     6481 cgggtagttc cagggggcga taccgacgat gaccccaatc gggtcgcggc ggacgaagga
     6541 ggtgtgatcc tcgaggtact cgccgccggc tcgaccctcc atgaagcgag cggccgccgc
     6601 gaagaaccgg aagttgtcga ccgtcaggtc gaactcgaaa tcgatgatcg acaccggctt
     6661 gccgacgttg agcgactcga ggcgcttgag ctcgggcagg tcggcctcga tcgcgtcggc
     6721 gagcttcgtg atcatctcga accgctcgcg gggtgtggtc ttccgccagg actcgaaggc
     6781 cgcgctggcg gcctcgacgg cgtcggcggc atcggcggca tcactcgacg ccacttcggc
     6841 gatgacctca cccgttgccg ggtcgagcac ctcgtcgacc gcacccgagc gtgctgcccg
     6901 gaactcgcca ccgatgtagt tgagcgtctg cacctgggtt gccatccccc gagtctgccc
     6961 cactcccgcg ccccacaccc cccaccccga ccggagcgct cagttgctct ggagcgctgg
     7021 tgtgacagtt cctgtcacct gaccgctcca gtcgtcgtcc cgatctgggt gtcagacctc
     7081 ccgggtacgt tgctgcacct tcccctgcac gtcgtcgaca tccccgggag cccccgtgtc
     7141 gccacccgac ctgcaggtcg cgcgcctcgc cgcgcgacag caccattgtt tcgcccgttg
     7201 ccaggcgctc gagatcggtc tgagctatcg acagatccga caccgcgtcg acgtcggccg
     7261 gtggacccag ctgcacgaca gcgtcttctg tctcgccggc gctccgatcg ccgcactcgg
     7321 acacatccac gccgcggtcc tggccctccc cggttcggcc gcgagccacg gcagcgcgct
     7381 ccacctgtgg ggcgaacgcg acccggcgcc gcgggtcgcg cacgtcatcc gccgccacgg
     7441 gcggagccat cgcctgcacg gggtccacct ccaccagacg cggctgcttc cacagcacca
     7501 cgtgacggtc gctcagggcg taccggtgac gacgcgggcg cgaacggtct tcgacctcgc
     7561 cgccacgacc gacctcgcga cgctcgggcg gctcatcgac cgccaggtgg tcgccggctc
     7621 gctgcgcctc gacgaactcg agctgatgct cgacgagatg gtctgcccgg gtcgccccgg
     7681 ggtcggccag ttcgcggcgg tcctcgacca acgagtcggc cacggtcccg gcgtgtcgat
     7741 gctcgaacag cggtaccgtt cgctctgcga acgacgatca ctcccgctgg gcgatcccga
     7801 ggcacgcgcc ccgtgggcga cgagtggtgg accgagatcg gaacgggtgg acttcgccca
     7861 tggccgcgag cgggtgatcg tggaactcga cggccgcacc taccacgccc ggctcgagga
     7921 cttcgagaac gaccgtcgcc gcgaccacct cgcgcttgcg gccggatggc gaaccgtgag
     7981 attcacctgg cgtcaggtga ccaccgatcc ggaccacgtc gagcggatcc tccggtccgt
     8041 gctggacgtc ggggcgtgac gctggagcgc tgaggtgaca gaaactgtcg catcagcgct
     8101 ccagagcaac tgagcgctcc ggtcggggtg gggtcaggtg ctacgactcg aggtcggcga
     8161 tggcggcggc gatggcgaga gctcgccggg cgttgtcgac gtggaggttc tcgatcatgc
     8221 gaccgtcgac ggtgatgaca cccttgccgg ccgcgatgcc ctcgtcccac gcggcgatca
     8281 cgcgctcgct gtgctcgacc tcggccgccg acggagcgaa cgcgtcgttg cacggctcga
     8341 tctgggtcgg gtggatgagc gtcttgccgt cgaaccccat ctcggcgccc tgcgcgcact
     8401 ccgccgcgaa cccctcgggg ttcttcacgt cgttgtacac accatcgagg atcaccttgc
     8461 cggcgaaccg ggcagcgttg aggcaccgac cgaggcccca caacaggggt gcccgacccg
     8521 gcacgagcgc agcgcgcagt tccttggcga gatcgttcgt gcccatgatg agcaccgtca
     8581 ggcggtccga cgacgttgcg atctcgaccg cccgctccat cgccgccggc gtctcgagca
     8641 tcgcccagat cgtggtgttg gccgcggcgt cgatgccgtc gagcacgccc tcgacgaggg
     8701 cgacgtcagc ggccgagttg atcttcggga ccaccacagc ggacggtgcc gcggcgccgg
     8761 ccgactcgag gtcggccttc caccactcgg tgtcgagacc gttgatgcga atcgtcagct
     8821 cgcgccggcc gtactcaccg ctgcgcaccg cggcggcgac cttctcgcgg gcgaccgcct
     8881 tcatgtcggg ggcgacggag tcctcggtgt cgaagatgat ggcgtcgcac ggcagcgtct
     8941 tcgccttctc gagggccttg tcgttcgctc cgggcatgta gaggacggaa cggcgcgggg
     9001 tgatgatctc ggccatgtca gcctccgaat ccgtaggtgg cggcgagctc ggggtcgcgg
     9061 gcggacagca tcttggccag gttcaccatg acgtggcact gcttcaccga ggcgtcgtcc
     9121 tccatcttcc cgtcgatcat gacggcgccg gtgccgtcgc ccatctcctc gatgacgtgc
     9181 aacgcgtggg cgacgtcctt ggggtcgggc gagaacacct tcttggcgat gtcgatctgg
     9241 accgggtgca gcgaccaggc gcccacacag ccgagcagga acgcgttgcg gaactggtcc
     9301 tcgcaggcga ccgtgtcctt gatgtcgccg aacgggccgt agaacggcag gatgccggcg
     9361 gtcgcacagg cgtcgaccat ccgggcgatc gtgtagtgcc agagatcctg ctggtaggag
     9421 gtgcgagcgc cctggtagtc ggggccgtcg gcggtctgcg ccgggtcctc ccggacgagg
     9481 tagccggggt gaccgccgcc gacacgcgtg gtcttcatac ggcggttggc cgcgaggtcg
     9541 gcagggccga gggacaggcc ctgcatgcgg gggctggcga ggcagatctc ctcgacgttg
     9601 gcgacaccgc gagccgtctc gaggatcgcg tgcacgagga gcggcttggt gagcccggcc
     9661 ttcgcttcga gctgggccag caggcggtcg acgtagtgga tgtcctcggg cccttcgacc
     9721 ttcgggatca tgatcacgtc gagcttgtcg ccgatctcgc cgacgagggt gacgaggtcg
     9781 tcgagcaccc acggcgagtc gaggctgttg acccgcgtcc agagctgcgt gtcgccgagg
     9841 tccatctcct tgccgacctt cacgaggccg gcgcgggcgt tctccttctg gtcggccggg
     9901 atcgcgtcct cgaggttgcc gagcaggatg tcgaccgtcg gggcgatgtc ggggagcttg
     9961 gcggtgacct tctcgaggtg cggcgggaag aagtggatca tccgcgacgg cgggaacggg
    10021 atctcggtga cgggctgagg ggcgccggcc gcgaggggtt tgaagaagtc cttggagctc
    10081 cgcatatggc aatccaagtt agaggtcgcg cccgctacga atcgaacccc agcccgagcg
    10141 agtcgaggag gcggagccac gggccgcggc gaccctcgtt ggcgtcggcc ttggcgagcg
    10201 ccttgcgggt gagctcgatg ccggcccatc gcacgggctc gggcgggaac gggatcgcct
    10261 tgcgcttcac catgcggagc gacagcagct cggagtcggg gtggaacagc aggtcgaggc
    10321 agacccgagc gccgaacctc gtggcgccga cgccgaggcc ggtatagccg acggcgtacg
    10381 cgacccgtcc gtcgaacgag gtgccgaacg tgaccgagaa ccgggtgcag gtgtcgatca
    10441 ccccgcccca ccggtgactg aacgagaggc cctccagctg cgggaacgtc tcgaagaagt
    10501 gctcggcgag gacctgatgg gtctcgtcgc gttggtcgag cgacggatcg atcttcgacc
    10561 cgaagtggta gatcgcgtcg tagccgcccc acaggatccg gttgtcggcg gtgagtcggt
    10621 agtagtggaa ctggttggtg gtgtcgctga tgccctcgcg cccgctccag ccgatcgacg
    10681 cgagctggtc ggccgacagc ggctcggtcg cgagcacata gtcgtagacc ggtgcgatgg
    10741 tgcggttgat cgcaccgatc ggcgacttga aggcgttggt gcccagcacc accgcgccgc
    10801 agtggatcgt gcccgactcg gtgcggaccg ccatgccggc gcccgatcgt gccagcccca
    10861 gcatgggcgt gcgttcgtag atctcgacgc ccaaggatcg agcggcgtcg gccaggcccc
    10921 atccgagccg ggccggatcg acgatggcct caccggttcg ctgcaccacc ccggcctcgt
    10981 aggtgggcga tgcaacacgg tcgcggatcg cgtcgcggtc gaggaactcg acgtcgtggc
    11041 cgaaccggcg ggtgagctcg acctgctcgt ggaggtcgtc gaccagccag ggggcggtcg
    11101 caaccgtgag gccgccggtc ggctgccagt cgcagtcgat gccgtaccgc tcgatcgtgg
    11161 ctgcaattcc ggcgaagttc tcggtcgcca actcctcgat gcgcgccatc tccgacggga
    11221 accgggcgac gccgttgtcg agcccgtggg tcagcgacga cgagcagaag ccaccgttgc
    11281 gtccagacgc ctgttcggag acgcggccgg cctcgaccac caccaccgtg cgggaggggt
    11341 cgtcctcctt ggcctgaatg gcggcccaga gcccggtgaa tccggcaccg acgatcacca
    11401 ggtcggcccg cgtgccgccg cacaacgtgc gaccgggatc gggccggtcg ggagtgtcga
    11461 gccacagcac ctcggggcgg gcgtcgacca gcgcccggtg ggcgcgggtg gaagcggaac
    11521 tcatggtccc gtcagcgtac cgacgccgac gggaccgaac acggcctcgg gccctgcgac
    11581 ggaggctcgc tcccgactcc gtcgggaact tctcacggcc ttgaccctcg gcggttggtt
    11641 caggagcccc cgggcaggac gtccttgagc ttgtcggcag cggtgtcgat ctgatcgctg
    11701 tacttgccgc cggtcttgtc gtcggccagc tcggccgcct tgtcgatgcc ctcgtcgacc
    11761 ttgtcggcgt gctcctcgag caggcccttg gccttgtcgg tgatgtctcc gagatccatg
    11821 tcgcgctctc catttcgccc accaactcgt ttggtgttga acggattgtt gcaggtgtcg
    11881 cgaccgccgg gtcgtcgatc ggcatgagca aaatccggcc gtgaggcgct gcggaaccgg
    11941 cgccgacgga gtaggaagtc ggcatgactg aacgcccccc cgggatgtac gtcggcgaac
    12001 tgaccgacgc ggccacccac gagcgcgccg gcacgccgct gggcctgaac cccgacgact
    12061 tcacgaccca cggtgtgatc gtcggcatga ccggttcggg caagaccggc ctcggcatcg
    12121 tgctgctcga ggaagcgctc accgccggcg tgccgacgct catcctcgat ccgaaaggcg
    12181 acatgggcaa cctgttgctc acgttcccga acctcgcccc cgacgacttc gcgccgtggg
    12241 tgcctgccgg ccaggacgcc gccggcgtcg ccgacacgtg gacgaagggc ctcgccgact
    12301 ggtcggtcgc gccgaccgag atcgccacgt tgcgcaccgg tcaccgcatg agcgtctaca
    12361 caccggggtc gacgtccggc atcccgctca acatcatcgg gtcgctcgct ccccccacga
    12421 attcgacagg cggaactcct gatctcgaag cgatccacga cgaggtcgag tcgctggtgc
    12481 agggcctgct cggcctcgtc ggggtcacgt cggatccact cagcggccgc gagcacgtcc
    12541 tcatggccaa catcatcgag ctcgcgtggg cggccggtga gaccctcgac ctggcgaccc
    12601 tgctcatccg gatccaggac ccgccgctcc gcaagctcgg tgtcatcgac gtcgagacgt
    12661 tcttccccaa gcccgaccgc accgccctca tgatgaagct caacggtctg ttggcgtcac
    12721 cgtcgttctc cgcgtggggc cacggcgcgc ccatcgacat cgagtcgctc ctgtggtccg
    12781 acgacggcac cgccaacgcc gcggtgatct acctcgccca cctgtccgac gaggaacggc
    12841 agatggtcgt gacccgggtg ctctcgaagc tcgtcgggtg gatgcgcggc cagcccggct
    12901 ccacgaagct ccgggtgctc gtctacatgg acgaggtgta cgggttcgtg ccgccgacgg
    12961 cgatgccgcc ggcgaagaag ccgatcctga ccctcttcaa gcaggcgcgg gcgttcggcg
    13021 tcggggtggt gctcgccacg cagaaccccg tcgacctcga ctacaaggcg atctccaacg
    13081 ccggcacctg gatgatcggc cgcctccaga ccgagcgcga caaggcacga ctcctcgacg
    13141 gcatgagttc ggcggccggc accgtcgacc tcgccacggt cgacgcgacg atctcgtcgc
    13201 tcgcgaagcg cgagttcctg ctccacacca ccggcggcaa accgccccgc accttcggtg
    13261 tgcggtgggc aatgagttac ctcgccggcc ccatcagcaa agaccaggtc ccgagattgc
    13321 ccgggcaggc gtcggcgccc gctcccgagg acgccggcga tgcgtcgtcg ccccgctacc
    13381 gcgaaggggc tccgactgca ccgtcgacgc cctcgtccgc tccggtcgac gcggccgagc
    13441 cacctcagga cgggcaggct gacaccgccg gctcgtcggg ggctgggggg ttggcgcatg
    13501 acgagactcc cgccatgcct gccgttgcag ccggggtccc cgtgcggttc ctggaccccg
    13561 cggcgccgtg ggctcccacg ctcggcgccg tttcgggtgg gcggcggctg caggcgtgtg
    13621 ttgcggtgcg cgtgaacctg ttgttcgacg acaccccgtc gggtctgcgc cacaccgaac
    13681 agatcgaggc catcttcgcc ccgctcgatc agccgaggat cgatccggcc aaggcgtaca
    13741 gcgtcgacta cgacgaccgc gacctccaga cgtccgctcc ggccggcgcc gtgttcgtgc
    13801 tgccgtccgc gcccgtgaac accaaggcgt acttcaacga cgtcgagtcc tcggtgaagg
    13861 actacctgtt ccgctcccgc cacacgacga tctacggcaa taagtcgctg aagctctaca
    13921 gccgccccgg cgagagccag gccgacttca cggctcgctg ccatgccgcc gccgacgccg
    13981 gcgccgaccg cgacaccgac cagatccgcc agaagctgca gctcaagatc gaccgcatca
    14041 acgagggcat cgccgccgat cgtcaacgtg cccagcaact cgaggaggag gcggccgcat
    14101 ctcgccgcaa cgaggccgtg agcatcggcg gcagtgtgct cggtgcaatc ttcggaggcc
    14161 gaaagagcgc ccgttcgatc agcgccgcgg ccaaccgcgc cgccacgggc cgggcgcggt
    14221 ctcagaaggc cgacaaccgg atggaacagg cgctcgcccg tatcgacgag aagatggcca
    14281 acatcgacga cctccaacac caactggccg acgagatcac cgacatcgtc gcccactggt
    14341 ccggcctcgc ggccgacgtc gtcaccctcg agatccccct cgagaagtcc gacatcggca
    14401 tcgagcaggt cgcgctggtg tgggtgccga cggcttgacg gcctcggccg tcggctacag
    14461 gggagggcgg tcgccgagtt cggggaaatg acacgccacg cggtgatcgg tcgcgtcgcc
    14521 gtcgacgggg accagcggcg gctcggtggt cgcgcacagg tcggtcgcct tccagcagcg
    14581 agtccggaac cggcagcccg agggcggatc gatcggtgac ggcacctcgc cggtgagcac
    14641 gatgcgtttg cgggcacgtt ccttgcgggg atcggggatc ggcaccgccg acaagagggc
    14701 gttggtgtac ggatggcggg gctgggtgta gatctgcgcc ttggtgccga cctcgacgag
    14761 cttgcccagg tacatcaccg cgacccgatc cgagatgtgg cggaccaccg acaggtcgtg
    14821 ggcgatgaac aggtacgcga tgccacgttc gtcctgcagc cgttcgagga ggttcacgac
    14881 gcccgcctgg atcgacacgt cgagcgccga gaccggctcg tcgagcacga tcacctcggg
    14941 gtcgacggcg agggcacggg cgatgccgat gcgctggcgc tgaccgcccg agaactcgtg
    15001 ggggtaacgg ctccggtgct cgggtgacag gcccactgtt cgcaacagct cgctgacctc
    15061 ctcggtgcac tccttcgcct tcttgccctg gtggacgagg aacggctcgg ccagcacctc
    15121 gccgatcgtc atgcgggggt tcagcgacgc gtacgggtcc tggaagacca tctgcgcccg
    15181 gcgccggagc tccttgttgg gcatcttggc ggtgtcctga ccgccgagct tcacggtgcc
    15241 cgcggtggcc gtgaggagac ggagcaccac acgcccgaag gtggacttgc cgcaaccgga
    15301 ctcgccgacg atgcccaggg tctcgccgcg gtcgacgctc atggacacac cgctgacggc
    15361 gtgcacctgg ccgatggtgc ggccgaacag cccacccgtg accgggaact ccttgacgac
    15421 gtcgagcgcc tcgagaacgg ggctgttgcc ggcggacggc gacggcgccg acggcgggat
    15481 gggttcggtc acggtcatgc tcccaccact ccgaggagcg cacgcgactc gtcgcgacgc
    15541 caacaggcgg cgccgtgacc ggagcggtcg acggcgggac ccgtggtgcc gctgacgatg
    15601 gccgccggcg tctcgtcgaa gagggtcggc atcatcgagt cgcacccgga ctcgctccga
    15661 gcgatcgcgc accgcggatg gaacgcacaa ccggccggcg ggttgagcat cgacggaggt
    15721 tgaccgccga tcggcacgag ctggtcgtcg ccgtcggcat cgacgcgggg cagcgagcgc
    15781 agcagaccga tggtgtacgg gtgagtcgga tcgtcgaaca gttcgtcggt ggacgcctgc
    15841 tccaccactt tgccggcgta catgacggcc accttgttgg cgaggccggc caccacgccg
    15901 aggtcgtggg tgatgagcat cacagccgca ccctgttgcc ggctcaccga cacgagcagc
    15961 tccagcacct gcgcctggac ggtcacgtcg agggccgtgg tcggctcatc ggcgatcacc
    16021 aacttggggt cgcacgacag cgccatcgcg atcatggccc gctggcgcat gccgcccgag
    16081 aactcgtggg gaaagttgtc gacgcggcgc tgcggctgtg ggatgccgac cagctcgagc
    16141 atctcgatcg cccggttctt ggcgtcggcc ttcgagatct gccggtgcga ctgcaacatc
    16201 tcgatgatct gcttgccgac cgtgtagacg gggttgaggg cgctcagcgg gtcctggaag
    16261 atcatggcga tctcaccgcc gcgcacctgg cggagccggt cacccttggc gccgatgagg
    16321 ttctcgccct cccactcgat gcgccccgtc atgttggtgc gcccctcggg gtgcagcccc
    16381 agcacgctga gggccgtcac ggacttgccg gaccccgatt cgcccacgac ggcgaggacg
    16441 tcgccgtcgt cgatcgacat gtcgacgccg cggaccgcct gcacgacgcc gtcctcggtc
    16501 cggaagccga cgtggaggtc ggagatctcg agcagtcgcc ccatgtcagt tgtccttcac
    16561 gtcgagggcg tcgcggaggc cgtcccccac gaggaggaac ccgagcaccg tcaacacgat
    16621 ggccagaccc gggaacagga acatgtacgg ggcactggtg aggaagctct gggcgtcgga
    16681 caccatcagg ccgagtgacg ggttcggcgg cttcacaccc acaccgagga agctcagggc
    16741 cgcctcggac aggatcgcgc tgcccacgcc gaccgccatg aggaccacca cgggctggag
    16801 cacgttgggg aggatgtggc ggagcatgat gcgggggccg ctgaccccga gggcgcgggc
    16861 ggcctcgatg tactcgaact ccttcacctg gatgaaccct gcacgaacgg ttcgggccgt
    16921 ctgcagccag gccgtgaagg ccagagcgaa gatcaccgcc ggcacgccct tcccgaagac
    16981 ggtgatgaag gcgtaggcga ggatgatgta cgggatgccc agcatgatgt cgacgaagcg
    17041 catggcgacc gagtcgaccc acttgccgta ccaaccggca agcgcaccga cggcgatgcc
    17101 gatgaacgtc tcgagcaacg tcgccatcac gccgatgaac agcgacagcc ggatgccgtg
    17161 gacgacgcgg gcgaagatgt ccgcaccctt gttgtccacg ccgaacaggt gctggttggt
    17221 cgggggttcg aggatgacgg tcttcgacag cttggtcggg ctctggacca cgaaggggcc
    17281 gatgatggca accaggacca ggaagatcac caggaacagg ccgaacatcg ccaacttgtt
    17341 gcgacggaac cgtcgccagg catcctcgaa cagcgagcgt gagggcccga cgtcggcgag
    17401 cgtggcgtcg agagcgacga ccccgatcgg cgtgtcggcg accggctgct gtgcaggcac
    17461 ctcgggagtg agtctgggat cggtcatttc gcaccgctca gtcggatccg cggatcgagc
    17521 ttggcgtaac tgatgtcgac cgccaggttg gcgaggccgt acatcagcat cacgaccatg
    17581 gacaacgtca ggacgaccgg caggtcggac tgacctgcgg cacgggcgac cttggagccg
    17641 aggccgggcc actggaacac ggtctcggtc aggatggcga acccgatgag cgtgccgaag
    17701 tcgatgccga tgaacgtcac gacgggaatg agcgcgttgc gcagcacgtg acggcgggag
    17761 atcgccttct cggtgaggcc cttcgcccgg gcggtgcgca cgtggtcggc gctgagggcc
    17821 cactcccgct cgagcagatc ctc
//
LOCUS       DAOSOA010000021        17468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3274_length_17468_cov_7.899156, whole genome shotgun sequence.
ACCESSION   DAOSOA010000021 DAOSOA010000000
VERSION     DAOSOA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17468
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3274_length_17468_cov_7.899156"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKY13_02840"
     CDS             complement(<1..185)
                     /locus_tag="PKY13_02840"
                     /inference="COORDINATES: protein motif:HMM:NF028194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HOT78892.1"
                     /translation="MTETNRAVVLKQRPQGLLTWDDLEIVEQPRPELQEGEALIETML
                     LGMDATVRTWLNRGEGYL"
     gene            complement(225..1595)
                     /locus_tag="PKY13_02845"
     CDS             complement(225..1595)
                     /locus_tag="PKY13_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78893.1"
                     /translation="MTIVASVLVPLSVLTPVASAAPTETSALTLTDVSGARIARNGAP
                     GVVHAQVNNTGAAAAADVRVRYRLPSGVQLVPASSTAGCTAAGSIVTCALGTLAAGTS
                     RSIDLAVREPLATAVLGSRVGYFFQPTSDAYDPSSGEIRMSTWYHDAGAQGTDLGLCW
                     PLANETPNMDVAGGVCDGANDTAALEPDEVAVLDAFPEPFAESLVRSWQFDTEITPQQ
                     SGSYRACGLAIDDGGYLAVAPVGTPLTPADIRLNVPSFSSATGDPFDLVAGQRYRVLM
                     RITNRGGPGVDNGANGGALAGWDAYGIVPADETCDLGAAGVFGTADSAWVKRSVADVV
                     VVGSSDLTVLGAVESAPVDGVRSATVRVGNTGGDTTAARLAFELPTGSRLTRPPTGCD
                     RVLTGPPPTCEVDAISPTTTVGVGLVVSVGFSGDTPGVGWNLTAVSTVDADPADNRGS
                     LASPPG"
     gene            complement(1767..2444)
                     /locus_tag="PKY13_02850"
     CDS             complement(1767..2444)
                     /locus_tag="PKY13_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT78894.1"
                     /translation="MDVREALYTTRAMRRVTPDPIPDDVQARILDAAIRAPTGGNAQN
                     WRFLLVDDPEVRAQIGPIYRRCIAVLWESFYADRVAAAEADPDSDDSKTFVKVRRSAQ
                     WLADNFEIYPLLLFSFVQHDPTGGSIYPATWSAMLAARAEGVGSSLTSVLLFENDTVL
                     DVLGVPAGEGWLMSSCVTFGYPTGRWGVAARRPAHEVAYRNRWGADVGLSIDTPLWPD
                     RDVPPAG"
     gene            2667..4364
                     /locus_tag="PKY13_02855"
     CDS             2667..4364
                     /locus_tag="PKY13_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOT78895.1"
                     /translation="MGMLRSRGWRLVGSVALAATLTAAGCANDTDTASTPTTAASTGD
                     DRSTTTAEATTTAPERSCDQEPTVSPVEAAPVAATPSDVDVTSFDGTTIRLHWFPAPG
                     DDRHPTVLMGPGWSQPGSTLAQDPILFGALGIAGMNDAGYNVLTWDPRGFGESTGLAS
                     VNDPEREGRDVQVLLDWVAEQPVAVVDRPGDPRVGMVGASYGGGIQLTVAAIDCRVDA
                     LVPSLAWHSLRTSLHKSETVKAGWAGFLNTAAVTFGGRLDPHISSAAESGVGQGILSD
                     EDREWFISRGPGDLIDQVSVPTLFVQGTVDTLFTLDEAVVNYESLRSRNVPTAMIWFC
                     GGHGTCLTDEGDATLVNERSFAWLDKYLKGNDSADTGDRVDVVDQNAKHWTAADYPVE
                     LADPVTAEGAGELTLDATSAAGPLAAPRSGANDILSGSVLAITPSKAATALEVKIDPG
                     KVDALALGAPRLEMSYSGTTPAGDRPTRVFAQLVDDDTGIVLGNQITPIDIVLDGTQR
                     DVSVDLELISFHLQPGHTVTLQLVATTVAYAQPRLGGSLDASKISISLPVAKDVTPG"
     gene            complement(4377..5348)
                     /locus_tag="PKY13_02860"
     CDS             complement(4377..5348)
                     /locus_tag="PKY13_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT78896.1"
                     /translation="MAEMEYRRLGSSGLKVSVLSFGSWVSFGTQMDTGLARDCIAAAW
                     DAGVNFFDNAEAYAGGESERIMGAALAELGYPRNEYVVSSKFFWGIGSAPNMANTLNR
                     KYLHQAIDGSLERFGLDFLDLVYCHRPDPNTPIEETVWAMSDMVSSGKALYWGTSEWS
                     ADEIRAAWHIADKHHLHKPQMEQPQYNLFERNKVQREYARLYEDIGLGLTTWSPLASG
                     LLSGKYVDGVPDGSRATLPGYEWLRDLLTDPDSNAKVRQLSVVADELDCTLSQLAIAW
                     CAANPNVSTVITGASNPDQVRENMGALDVLGKLDAGLLGRIRSIAKG"
     gene            5495..6067
                     /locus_tag="PKY13_02865"
     CDS             5495..6067
                     /locus_tag="PKY13_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOT78897.1"
                     /translation="MTRPASPRLEPTTSDEWDDETVALLDAVGHLNIFTTLAHHPKLL
                     KRWMVFGGHVLGKNTLTARDRELLILRAGWRCGCAYEFGQHTVIGRQVGLTDDEITRA
                     ATEGYDGWGDREAALISATDELIADHMVSDTTWQRLETDLGSQQMLDLIFTVGQYTMV
                     CMALNSLGVELDAGVPGFPDGHAPDDGGPA"
     gene            6064..6876
                     /locus_tag="PKY13_02870"
     CDS             6064..6876
                     /locus_tag="PKY13_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT78898.1"
                     /translation="MTRMADKVAVVVGAGQTEGETIGNGRAIAIRLAQEGATVLAVDR
                     DLASAEETCWMIRDLADGPSAEATEAFRADITDEQACAAMAQHAVQAFGRIDVLVNNV
                     GIGAGDGGPTSLEADVWDRIHDVNLKGMWLSCKHVLPHMKGRQSGSIVNISSIAAVCS
                     APLLAYKTSKAGVNALTESLAMAGARYGVRANAVMPGLMDTPMAIEAIAEATGHDRDL
                     IRSERAKAVPLRREMGTAWDVANAVLFLASDEAAFITGAILPVDGGQSARIG"
     gene            complement(6919..7602)
                     /locus_tag="PKY13_02875"
     CDS             complement(6919..7602)
                     /locus_tag="PKY13_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78899.1"
                     /translation="MSSTTPPTDSPGIVVRNVSKRYGRTVALDHVDLEVARGEFAVLA
                     GPSGSGKSTLLNLLAALEKPDTGTIEVGGHLLGRHARHLNHLRRVEVGLVFQLHNLIP
                     RLTALENIEAAMFGTHHGRKARVARAHELLDRLDLSHRANERPPTMSGGERQRVAVAR
                     ALANEPKVILADEPTGSLDDESADLVLDLFDELRAGGATILAVSHDARLDRRGDRIVS
                     ITDLNCAVQ"
     gene            complement(7595..8713)
                     /locus_tag="PKY13_02880"
     CDS             complement(7595..8713)
                     /locus_tag="PKY13_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT78900.1"
                     /translation="MTFVGLIARNIISRPLRAVLTASAVAIGVMAVVALGVLTTSLKE
                     TATQILDVGNADFTIAQKNSSDIINGTIDAEDIAAIGTIPGVERAVGALISTDAYDES
                     NPLVIEVGLAPADQGPFGVNILDGESYGPDATDQVMLGYVLAKQIGKKVGDTVTVDGH
                     DYQVTGLYRTNISFGNSTMMFPLSYLQGANRLSGQVTLGFVQVAKGADIKKIRATIDG
                     DYQQLTTIETAEDFGRADRNLTLIGAANTGGSLLAGLIAVTGVLNTSLLSFFERIREF
                     GIYRSIGWSRSRVIALVLGEAIVVSIVGAAVGVFLGWGAINVLENLKQLKGIFTPTYD
                     AAVFGRSLGFGLVVAFLGALYPALRAAFVAPITAVRRE"
     gene            complement(8801..10216)
                     /locus_tag="PKY13_02885"
     CDS             complement(8801..10216)
                     /locus_tag="PKY13_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOT78901.1"
                     /translation="MPVPRHHLSAELTTGFARIRADLGVQEEFPPEALALAGKRASSP
                     SNANRDHRDRQDLPLLSIDPEGSRDLDQALHLERDGSGYVFHYAIADVAHWVQAGDAI
                     DLEARRRGTTIYCPDRRIPLHPTELSEGAASLLEGQSRPAILWTIRLDRDGATTDVSV
                     ERAIVRNHRALSYAAVQAAIDAGSTDEQFGLIEEVGRLRLTQEIERGGVSLELPEQAV
                     DLVGDHYELRYDEALPVEGWNAQLSLCCGMAAAQLMLDARCGILRTLPPADAETIARL
                     RVHSAALDVPWPDDLSYPEWVRTLDPATPHGAALMTQAARTLRGSGYLAFDGDVPAEH
                     RHHAIAADYAHVTAPLRRLVDRFANECVLAAAAGTRPPAWVLEALADLPGIMGSTGRL
                     ANQVERAVVDLAEAAALAHLVGHTFDAVVTSTGKGYSAIQLIEPAVIGTVPGELPIGA
                     STRVLLETADIAGPAVRFVPA"
     gene            10257..10952
                     /locus_tag="PKY13_02890"
     CDS             10257..10952
                     /locus_tag="PKY13_02890"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT78902.1"
                     /translation="MNTQPGLDRPVRVGTFNLHHGAPPERWFDRSGLAESARRLGVEL
                     LALQEVDRRVPRSRFSDGAAIVAKATGLHPTFASARRLQVTGQYGIALLTPQPPTAVE
                     VVRLPAFGSEQRLAIIASVELGGRRVSVASAHLHNRRWIAARQLPVVLDRLMARPGPH
                     LLLGDLNAGPDVLGPTVAAAGLSAVEGPPTFPTRGATDHIDWIAVGGLTASAVEVPSI
                     WASDHFPLVATLH"
     gene            11066..11941
                     /locus_tag="PKY13_02895"
     CDS             11066..11941
                     /locus_tag="PKY13_02895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT78903.1"
                     /translation="MATEITADMVIEVSEVVPRHPADLTGDLVIDLRPELDDTITESS
                     RADIADGVDAEAQTVPAAPGPLHGRVAVVTGGANGVGREVALGLARSGARVCVLGREL
                     AQLRETVELAGPEAAILFLQCDVGSLSEIEGVVDFIERFDRPVDILVHAEGVQVRGGI
                     EHGSVNDLDEQYLVNVRGPYLMSQRLLAQLRAGRGHVVFVNPAPSSEGAIDFGQFAVT
                     TDSVRSLADTLRREVQTSGVRVTSIRPEAPTTDVAAETVVALSPHDIADSVLHAVQVP
                     GQVEITDITLRPRSA"
     gene            11997..12971
                     /locus_tag="PKY13_02900"
     CDS             11997..12971
                     /locus_tag="PKY13_02900"
                     /inference="COORDINATES: protein motif:HMM:NF023430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HOT78904.1"
                     /translation="MGWSCRRPGCGREAEVCISYDALACQVWLDPIAMAPAGGQPLCA
                     DHAQRLSAPRGWVVLDRRTSQSTLMTAAGPAPVHDARGRAAVPRRRLPRAWGQFDAPR
                     LEFLAEGDDVVTEPEPTFEPPVVAATVHLEPALIEPEPVVEPEAEPEPAAVVIHAEGD
                     VSRRDLHGFPTADSSEPEAEAVGPEPEPVVEPERAPTAVVAPEAEPVGVVIHAEGDVS
                     RRDLHGFPTADSSEPEPELVVEPEAEPIAVVEPEPVAEPEPEPEPVVKKAPARRSRAR
                     AETGPLGKPKGRLLSRAFEASGPQRSAITESLEALGDDDGSTIEAAEG"
     gene            12992..14179
                     /locus_tag="PKY13_02905"
     CDS             12992..14179
                     /locus_tag="PKY13_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT78905.1"
                     /translation="MTSTGADTRIDPRLVLLLAAACGLAVANLYYAQPLLHTIAEDFG
                     TSSGTAGLVVTLSQVGYAVGLALIVPVGDLLDRRRLVPAILVGTAVATVAAALAPGIA
                     VLIAVAAFIGLGSVAAQLLVPLAAELASEEERGGVVGRVMSGLLLGILLARTVAGLVA
                     GVAGWRAVYWTAAVVATGLAIVLARALPADGPRPSRSYGDLLRSTARLFVSEPVLRRR
                     AVFGAFGFAAFSVFWTTVAFELSGPRYGYGDSVIGLFGLVGAAGALCAGFAGRLADRG
                     RANISTVAFAGSIMVSFLPLWWGRTSIVWLVVGIVVLDVGVQGLQVTNQSVIYGLPVA
                     RSGVNSVYMVCYFSGGALGSAVAAGLYDSGGWSSICLLGAGLGALVTVGALPGLRPTP
                     VTR"
     gene            complement(14186..15142)
                     /gene="ligD"
                     /locus_tag="PKY13_02910"
     CDS             complement(14186..15142)
                     /gene="ligD"
                     /locus_tag="PKY13_02910"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOT78906.1"
                     /translation="MASTPDEVRDGVGLTNLDRPLFDGAGVTKRDLVDYLDTVHERLI
                     PVLRDRPLSVVRATDRQRPFMQKNVPKYTPDWIETVEIWAEASQRAVRYALCNDRRTL
                     LWFANQRAVEYHPTLGRAGSLHHATDLILDLDPPTEGGFAGAVAAAELVRLALDDVGL
                     AAAVKTSGAKGVHVFVPVDPDHDPQDVAAATRAIARRAELLDPDVATTAYIKADRRGK
                     VFVDSTRSGGATVAAAYSPRARPGVPVSFPLEWDELDDVSPGDFTVLTAVALMEHRTP
                     WVESLPASQRLPDQLIAEGHGIPVARVAAMQEGKRRKRATEE"
     gene            15319..16197
                     /locus_tag="PKY13_02915"
     CDS             15319..16197
                     /locus_tag="PKY13_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78907.1"
                     /translation="MRRLSTLATLTAVLALFAAACTPGGPAPITWKVKPATIQVISKN
                     SQVTAGDRPYVIQVGFRSKLGVAGSSSAQISSQCSSGALPAPDAAPAGTTVGIPAGTA
                     DIPFPDAQNLDIGDVLLSTAPFEIFGTLSFAVNRNAPPFVTGCAMTDALNGALVGVLK
                     SSLDVLIAGSAVPPTQEQLIDLIVSNLGNFLSAAGSLIAAIIEGLGNPDAIVGVGVQL
                     LLPTAGAFTGLLDTAFGVAGLFSPELANGFIPIPSLPSAIKIKVGPLAPSVSVFDFDS
                     SDSGGGHWVYTSTIGV"
     gene            16260..16847
                     /locus_tag="PKY13_02920"
     CDS             16260..16847
                     /locus_tag="PKY13_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT78908.1"
                     /translation="MATQQTRRADTRVRLLAAAAELFAERGVDSTSIGAIAELADRTS
                     GAVYDHFGGKDGVLVALLDSWVVDLSAVIGAELATATTLEERLAVLWRNVSAPAVGDG
                     RWIALELELWTHASRDPAAREHLARRYRGAWRGIVELADASNDVSPDVGPAVIGLLFG
                     LEMMRRVDPVAVTDRLAIDSLTALVQRHRSDATTR"
     gene            16863..>17468
                     /locus_tag="PKY13_02925"
     CDS             16863..>17468
                     /locus_tag="PKY13_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT78909.1"
                     /translation="MRTQLCDDLGIEFPIFAFTHCRDVVVAVSKAGGFGVLGAVGFSP
                     EQLEIELNWIDEHIGDHPYGVDIVIPNKYEGMNEDMSAEDLTKMLQEMVPQQHLDFAR
                     KLMTDHGVPLPEGEDDNALKLLGWTEATATPQVEIALRHPKMTLIANALGTPPKDMID
                     HIHAEGRKVAALCGSPYQALKHADADVDIIIAQGGEGGGHCG"
BASE COUNT         2540 a         5886 c         6250 g         2792 t
ORIGIN      
        1 aggtatccct caccccggtt gagccaggtg cggacggtcg cgtccatgcc gaggagcatc
       61 gtctcgatga gcgcttcgcc ctcttgcagt tcgggcctcg gctgttcgac gatctcgagg
      121 tcgtcccacg tgagcaggcc ctgcgggcgc tgcttgagta ccacggctcg gttggtttcg
      181 gtcatggccg aaacgtagcc cgactggccg cggctcggtg tgcgtcagcc ggggggcgag
      241 gcgagactgc cgcggttgtc ggccggatcg gcatcgacgg tggacacagc cgtcaggttc
      301 cagccgacgc cgggggtgtc accggagaaa ccgaccgaca cgaccagccc gaccccgacc
      361 gttgttgtcg gggagatcgc gtcgacctca caggtggggg gcggcccggt gaggacccgg
      421 tcgcagccgg tgggtggccg tgtcagccgg ctgccggtcg ggagttcgaa cgccagccgt
      481 gccgcggtgg tgtcgccgcc ggtgttgccc acgcgcaccg ttgcgctacg gacaccgtcg
      541 accggcgcgg actcgacggc gccgagcacg gtgaggtcgc tcgacccgac gacgaccacg
      601 tcggcgacgc tgcgcttcac ccaggccgaa tcggcggtgc cgaagacgcc ggcagcgccc
      661 agatcacacg tctcgtcggc cggcacgatg ccgtaggcat cccaccccgc cagcgccccg
      721 ccgttggcgc cgttgtcgac gccggggccg ccacggttcg tgatgcgcat caggacccgg
      781 taccgctgtc cggcgacgag gtcgaacggg tcgccggtcg cggagctgaa ggacgggacg
      841 ttgagccgga tgtcggccgg cgtgagcggg gtgccgaccg gcgcgacggc caggtagccg
      901 ccgtcgtcga tggcgaggcc gcaggcccgg tacgaaccgg actgttgcgg tgtgatctcg
      961 gtgtcgaact gccaggaccg cacgagtgac tcggcaaacg gttcggggaa cgcgtcgagg
     1021 accgcgacct cgtcgggctc gagcgccgca gtgtcgttcg ccccgtcgca cacgcctccg
     1081 gcgacgtcca tgttgggagt ctcattggcg agcggccagc acaggccgag gtcggtgccc
     1141 tgggcgccgg cgtcgtggta ccaggtggac atgcggatct cgcccgagga ggggtcgtac
     1201 gcgtcgctgg tcggctggaa gaagtagccg acgcggctgc cgagcactgc cgtggcgagc
     1261 ggctcgcgga cggcgaggtc gatgcttcgc gacgtgcccg cggcgagggt gccgagcgca
     1321 caggtgacga tcgagccggc ggcggtgcag ccggcggtgg acgatgccgg aacgagctgc
     1381 acccccgacg ggaggcggta gcggacccgg acatcggcgg cagcggcggc gcccgtgttg
     1441 ttgacctggg cgtggacgac tccgggggcg ccattgcggg cgatccgggc gcccgaaacg
     1501 tcggtgagcg tgagcgccga cgtctcggtc ggcgctgccg acgcgaccgg cgtcagaacg
     1561 gagagcggca cgagtacgga cgcgacgatg gtcatcccga tgacggttcg acggagcgac
     1621 gcggtgccgg gtcgtcgtgg tcggagatgg cgcataccaa cgccagtatg agtcgttttg
     1681 tcccgtaagg gaacggcgct cccacccgct gggtgaattc acccaccagg ggtcgggacc
     1741 cccgggcggc cccggatcga ggcccgtcaa cccgccggcg ggacgtcccg gtcgggccag
     1801 aggggagtgt cgatcgacag ccccacgtcg gctccccagc ggttgcggta cgcgacctcg
     1861 tgggcgggcc gacgggcggc aacaccccat cgtccggtgg ggtagccgaa cgtcacgcac
     1921 gacgacatga gccacccttc gccggcggga acacccagga cgtccaacac ggtgtcgttc
     1981 tcgaagagga ggacgctggt cagcgaactc ccgaccccct cagcgcgggc cgcgagcatc
     2041 gcgctccagg tcgccgggta gatcgagccg ccggtgggat cgtgctgtac gaacgagaag
     2101 aggagcaggg ggtagatctc gaagttgtcg gcgagccact gcgcggagcg ccgcaccttg
     2161 acgaaggtct tcgagtcgtc gctgtcgggg tcggcctcgg ccgccgcgac acggtcggcg
     2221 tagaagctct cccagaggac ggcgatgcac cgtcggtaga tcggaccgat ctgggcgcgg
     2281 acctccgggt cgtcgacgag gaggaaccgc cagttctgag cgttgccgcc agtgggtgcc
     2341 cggatggcgg catcgaggat ccgcgcctgg acgtcgtcgg ggatcggatc gggagtgacc
     2401 cgccgcatcg cccgggttgt gtacagcgct tcgcgaacgt ccatgtgatg tctccccctg
     2461 tggtcgggtc atcgtaggac ggctccgtcc cagctgttcc atccctcaca ggccgagccg
     2521 ttccccggcg gcccggtgca cgccggcttc gaggcgtaca ccgccctgct cggcgacgcc
     2581 gtcgaggtcg agctcgacat ccaactcctg cagccgaact aggttcctga tcccccggca
     2641 ggcggggacc acgaggggat cgcgacatgg ggatgcttcg aagccgcggc tggcggctgg
     2701 tcggttcggt ggcgctcgct gcgacgctga ccgcggcggg ctgcgccaac gacacggaca
     2761 cggcgtccac accgacgact gccgccagca ccggcgacga ccgctcgacg acgaccgccg
     2821 aagcgaccac gacagcgccc gagcgatcgt gcgaccagga gccgacggtg tcaccggtcg
     2881 aggccgctcc ggtcgccgcc accccgagcg atgtcgacgt gacctcgttc gacggcacca
     2941 cgatccggct gcactggttc cccgcccccg gtgacgatcg gcacccgacg gtgctcatgg
     3001 ggcccggctg gtcgcagccc ggctcgacgc tggcgcagga ccccatcctc ttcggcgcgc
     3061 tgggcatcgc cggcatgaac gacgccggct acaacgtgtt gacgtgggat cctcgcgggt
     3121 tcggggagtc gaccggcctg gcgtcggtca acgaccccga acgcgagggc cgcgacgtgc
     3181 aggtgctcct cgactgggtc gccgagcagc ccgtcgcagt cgtcgaccgt cccggcgacc
     3241 ctcgggtcgg catggtcggg gcgtcctacg gcggaggcat ccagttgacg gtcgcggcga
     3301 tcgattgccg cgtcgacgcc cttgtcccga gcctggcgtg gcactcgctc cggacgagcc
     3361 tccacaagtc cgagaccgtg aaggcgggat gggccgggtt cctcaacacc gctgcggtca
     3421 ccttcggcgg gcggctcgac ccccacatct ccagcgccgc cgagtccggc gtcgggcagg
     3481 gcatcctcag cgatgaggac cgggagtggt tcatcagccg cggccccggc gacctgatcg
     3541 accaggtgtc ggtcccgacc ctgttcgtcc agggcacggt cgacacgttg ttcaccctcg
     3601 acgaggccgt cgtcaactac gagtcgctcc gttcccgcaa cgtcccgacc gcgatgatct
     3661 ggttctgcgg cggtcacggc acctgcctca ccgacgaggg cgacgcaacg ctcgtgaacg
     3721 agcggtcgtt cgcgtggctc gacaagtacc tgaagggcaa cgactccgcc gacaccggcg
     3781 accgcgtcga cgtcgtcgac cagaacgcga agcactggac ggccgccgac tacccggtcg
     3841 aactggccga cccggtcacc gccgaagggg ccggtgaact cacgctcgac gcgaccagtg
     3901 ccgccggtcc gctcgccgcg cctcgctccg gtgcgaacga catcctgtcc ggatcggtgc
     3961 tcgccatcac gccgtcgaag gcggccacgg cgctcgaggt gaagatcgac ccggggaagg
     4021 tcgacgccct cgcgctcggc gccccccggc tggagatgag ctactcgggc acaacgccgg
     4081 ccggtgaccg gccgactcgg gtgttcgccc agctcgtcga cgacgacacc ggcatcgtgc
     4141 tcggcaacca gatcaccccg atcgacatcg tgttggacgg cacccagcgc gacgtgagcg
     4201 tcgacctcga gctgatctcg ttccacctgc agcccggcca caccgtgacg ctccagctgg
     4261 tcgcgaccac ggtcgcgtac gcgcagcctc gcctcggcgg ctcgctcgac gcgtcgaaga
     4321 tctcgatcag cctgccggtg gcgaaggacg tgaccccggg ctgagccggg gacgcgtcag
     4381 cccttggcga tcgaccggat ccgcccgagc agtccagcgt cgagcttgcc gagcacgtcg
     4441 agcgcaccca tgttctcgcg cacctggtcg gggttgctcg cgcccgtgat gacggtggac
     4501 acgttggggt tggcggcgca ccaggcgatg gccagctgcg acagcgtgca gtcgagctcg
     4561 tcggcgacga cggacaactg gcggaccttc gcgttcgaat cgggatcggt gaggaggtcg
     4621 cggagccatt cgtagccggg cagcgtggcg cgactcccgt ccggaacccc gtcgacgtac
     4681 ttgcccgaca gcaggccgga ggcgagcggg ctccacgtcg tgaggccgag cccgatgtcc
     4741 tcgtagagac gggcgtactc gcgctgcacc ttgttgcgct cgaagaggtt gtactgcggc
     4801 tgctccatct gcggcttgtg gaggtggtgc ttgtcggcga tgtgccacgc ggcgcggatc
     4861 tcgtcggctg accactccga cgtgccccag tagagggcct tgccgctcga caccatgtcc
     4921 gacatcgccc acaccgtctc ctcgatcggg gtgttggggt cgggtcggtg gcagtagacg
     4981 aggtcgagga agtcgagacc gaagcgctcg agcgagccgt cgatcgcctg gtgcaggtac
     5041 ttgcggttca gcgtgttggc catgttcggc gcgctgccga tgccccagaa gaacttcgac
     5101 gacaccacgt actcgttgcg ggggtatccc agctcggcga gcgcggcgcc catgatgcgc
     5161 tcggattcgc ctccggcgta cgcctccgcg ttgtcgaaga agttgacgcc ggcgtcccag
     5221 gcggcggcga tgcagtcacg cgcgaggccc gtatccatct gggtgccgaa gctgacccac
     5281 gatccgaacg acaacacgct gaccttcaga ccactgctgc cgagcctgcg gtattccatc
     5341 tctgccatct gggttccttc cgatcgtcgc tggcgacgtt agccatgtgg gcccgccgcg
     5401 gtcgtcggga cggaccggga gtcggccatg atggcacccg tccacgcgat ggctgctgcc
     5461 gatcggcgag gaccagagca catcgagggg gaccatgaca cgaccggcaa gcccacggct
     5521 ggagccgacg acgagcgacg agtgggacga cgaaaccgtc gcgctgctcg acgccgtcgg
     5581 gcacctcaac atcttcacga ccctggctca ccacccgaag ctgctgaagc ggtggatggt
     5641 gttcggcggg cacgtgctcg gcaagaacac gctcacggcc cgtgatcgcg agctcctgat
     5701 cctgcgcgcg gggtggcgtt gtgggtgcgc ctatgagttc ggccagcaca ccgtcatcgg
     5761 tcggcaggtc gggctgaccg acgacgagat cacacgtgct gcgacggagg gctacgacgg
     5821 atggggcgat cgcgaggcag cgctgatctc cgccaccgac gagctgattg cggaccacat
     5881 ggtgagcgac accacctggc agcggctcga aacggacctg ggttcccaac agatgctcga
     5941 cctcatcttc accgtgggtc agtacacgat ggtgtgcatg gcgctgaact cgctcggggt
     6001 ggaactcgat gccggggtgc cgggcttccc cgatggtcac gcgcccgatg acgggggccc
     6061 ggcatgaccc ggatggccga caaggtcgcc gtggtcgtcg gtgccggaca gaccgagggc
     6121 gagacgatcg gcaacggacg cgccatcgcg atccgtctcg cccaggaggg cgcgaccgtc
     6181 ctcgcagtgg atcgcgacct cgcctccgca gaggagacgt gctggatgat ccgcgacctc
     6241 gccgacggcc cctccgccga agccaccgag gccttccggg ccgatatcac cgatgagcag
     6301 gcatgcgccg cgatggcgca gcacgcggtg caggcgttcg ggcggatcga cgtgttggtc
     6361 aacaacgtcg ggatcggggc cggtgatggc gggccgacca gcctcgaggc agacgtctgg
     6421 gatcgcatcc acgacgtgaa cctgaagggg atgtggctgt cgtgcaagca cgtcctgcca
     6481 cacatgaagg gtcggcagtc ggggtcgatc gtcaacatct cgtcgatcgc ggcggtgtgc
     6541 tcagcgccgt tgctcgccta caagacgtcg aaggccgggg tgaacgcgct caccgagtcg
     6601 ctcgccatgg cgggggcgcg gtacggggtc cgcgccaacg ccgtgatgcc ggggctcatg
     6661 gacaccccga tggcgatcga ggccatcgcc gaggcgaccg gtcacgaccg cgacctgatc
     6721 cgttccgaac gtgcgaaggc ggtgccgctc cggcgggaga tgggtaccgc ctgggacgtg
     6781 gcgaatgccg tcctgttcct cgcctccgac gaggccgcgt tcatcaccgg ggcgatcctg
     6841 cccgtcgacg gcggccagag cgcccggatc ggatagcagc cgggccgccg ctctcgggcc
     6901 gccactgcgg gacgttcgct actggacggc gcagttgagg tcggtgatcg agacgatccg
     6961 gtcgccgcga cgatcgagac gggcatcgtg gctgacggcg aggatcgttg cgccgccggc
     7021 gcgcagttcg tcgaaaaggt cgagcacgag gtccgccgac tcgtcgtcga ggctgcccgt
     7081 gggctcatcc gcgaggatga ccttgggttc gttcgcgagc gcccgggcca cggccacccg
     7141 ctgacgttcc ccacccgaca tcgtcggcgg tcgctcgttg gcccggtgcg acaggtcgag
     7201 ccggtcgagg agttcgtggg cgcgtgccac gcgggccttg cggccgtggt gtgtgccgaa
     7261 catcgccgcc tcgatgttct cgagcgcggt gagccggggg atcaggttgt ggagctggaa
     7321 caccaacccg acctcgacgc gccggaggtg gttgaggtgg cgggcatgac gaccgaggag
     7381 gtggcctccg acctcgatgg tgccggtgtc gggcttctcg agcgcggcga gcaggttgag
     7441 cagcgtcgac ttgccggaac cggaggggcc ggcgagcacg gcgaactcgc cgcgagcgac
     7501 ctcgaggtcg acgtggtcga gggcgaccgt tcggccgtac cgcttcgaga cgttgcgcac
     7561 gacgatgccc gggctgtcgg tgggaggggt cgtgctactc acgtcgcacc gccgtgatcg
     7621 gtgcgacgaa cgccgctcgc agggccgggt agagggcccc gaggaaggcc accaccagtc
     7681 cgaaccccag cgatcgtccg aagacggcgg cgtcgtaggt cggcgtgaag atgcccttga
     7741 gctgcttgag gttctccaga acgttgatgg ccccccaccc gaggaagacc ccgaccgccg
     7801 cgccgacgat gctcacgacg atcgcctcgc cgagcaccag ggcgatcact ctcgaccggc
     7861 tccaaccgat cgagcggtag atgccgaact cacggatgcg ttcgaagaac gacaagagcg
     7921 aggtgttgag gacccccgtc accgcgatga ggccggcgag gagcgagccg ccggtgttcg
     7981 ccgcgccgat gagcgtcagg ttccggtcgg cgcgcccgaa gtcctcggcc gtctcgatcg
     8041 tcgtgagctg ctggtagtcc ccgtcgatcg ttgcccggat cttcttgatg tcggcgccct
     8101 tggcgacctg cacgaatccg agggtgacct ggccgctcag tcggttggcg ccctgcaggt
     8161 acgacagcgg gaacatcatg gtgctgttac cgaaggagat gttcgtccgg tagagccctg
     8221 tcacctggta gtcgtggccg tcgacagtga cggtgtcgcc gaccttcttg ccgatctgct
     8281 tggcgaggac gtatcccagc atgacctggt cggtcgcgtc ggggccgtag gactcgccgt
     8341 cgaggatgtt cacaccgaac gggccctggt cggcgggtgc gaggcccacc tcgatcacga
     8401 gtgggttcga ttcgtcgtag gcgtccgtgc tgatcaacgc cccgacggcc cgctcgacgc
     8461 cggggatcgt cccgatggcg gcgatgtcct cggcatcgat cgtgccgttg atgatgtcgc
     8521 tgctgttctt ctgggcgatc gtgaaatcgg cgttgcccac gtcgaggatc tgcgtcgcgg
     8581 tttccttgag actcgtggtc aggacgccga gcgcgaccac tgccatgaca ccgatggcga
     8641 cggcgctcgc cgtgaggacg gcgcggagtg gccgcgagat gatgttgcga gcgatcagac
     8701 ccacgaacgt catcgggtac tcctttcggc tcctcgagcg gcgcctgggt ccattcgatc
     8761 agatccgaca ccccggcgta cggatgatct tcggggtcga tcaggcggga acgaacctga
     8821 cggcggggcc ggcgatgtcg gcggtctcga gtagcaccct cgtcgacgcg ccgatgggga
     8881 gttcccctgg cacggttccg atgacggcgg gctcgatgag ctggatggcg ctgtacccct
     8941 tgccggtcga tgtcacgacc gcgtcgaacg tgtggcccac gaggtgggcc agcgctgccg
     9001 cttcggccag gtcgacgacc gctcgctcga cctggttggc cagccggccc gtcgatccca
     9061 tgatgccggg caggtcggcg agcgcttcga gcacccaggc cgggggacgc gttcccgctg
     9121 ctgcggcgag gacgcattcg ttggcgaacc ggtcgacgag tcggcgcagt ggcgcggtga
     9181 cgtgtgcgta gtcggccgcg atcgcgtggt ggcggtgttc cgcggggacg tcgccgtcga
     9241 acgccaggta ccccgagcct cggagcgtcc gggcagcctg tgtcatgagg gccgccccgt
     9301 ggggcgtggc cggatcgagt gttctgaccc actcggggta ggagaggtcg tcgggccacg
     9361 ggacatcgag agcggcggag tggacgcgca gtcgggcgat ggtctcggcg tcggcgggtg
     9421 gcagcgtccg gaggatcccg cagcgcgcgt cgagcatcaa ctgcgcggcc gccatgccgc
     9481 agcagagcga cagctgagcg ttccacccct cgacggggag cgcttcgtcg tagcgcaact
     9541 cgtagtggtc gcccaccagg tctacggcct gttcgggaag ttcgaggctg acgcccccac
     9601 gttcgatctc ctgggtgagg cgaagacgcc ccacctcttc gatcagcccg aactgctcat
     9661 cggtcgatcc cgcgtcgatc gcggcctgga ccgcggcgta ggacaacgcg cggtggttgc
     9721 ggacgatggc ccgctcgacg gagacatcgg tggttgcacc gtcccggtcg agccggatgg
     9781 tccagaggat cgccggccgg gactggccct cgagcaggct ggcggccccc tcgctcaact
     9841 cggtcggatg gagcgggatg cggcggtcgg ggcagtagat cgtggtgccg cggcgccgcg
     9901 cctcgaggtc gatggcgtcc ccggcctgga cccagtgcgc cacgtcggcg atcgcgtagt
     9961 ggaagacata tccgctgccg tcgcgttcga ggtggagggc ctggtcgagg tcgcggctcc
    10021 cctcggggtc gatggacagg agcgggaggt cctggcggtc gcgatggtcc cgattcgcgt
    10081 tcgacgggct cgacgctcgc ttcccggcga gcgccagcgc ctcgggggga aactcctctt
    10141 ggacgccgag gtcggcgcga atcctcgcga agcccgtcgt cagctcggcg gagaggtggt
    10201 ggcgtggcac gggcatgggg ccgaacccta gtgagcgagc cactaccgtc cgcctcgtga
    10261 acacccaacc cggattggac cgacccgtgc gggtcggcac gttcaacctg catcacggtg
    10321 cgccgcccga gcgatggttc gatcgctccg gcctggcgga gtcggcgcgc cgactcggcg
    10381 tcgaactgct ggcgctgcag gaggttgacc gacgtgtgcc gcgcagccgg ttctccgacg
    10441 gtgcagcaat cgtcgccaag gcgacggggt tgcatccgac gttcgcgagt gctcgacgcc
    10501 tgcaggtgac gggccagtac gggatcgcgc tgctcacgcc acagccgccg acggccgtcg
    10561 aggtcgtgcg gctcccggcg ttcgggagcg agcagcggct ggcgatcatc gcgtcggtcg
    10621 aactcggcgg ccgacgggtg agcgtcgcgt cagcgcatct gcacaaccgg cggtggatcg
    10681 cggcgcgcca actccccgtg gtgctcgatc gactgatggc gcggccgggt ccacacctgc
    10741 tcctcggtga cctcaacgcc ggtcccgacg tgctcggccc gacggtcgcg gcggccgggc
    10801 tgtcagcggt cgagggtccg ccgacgttcc ccacgcgtgg ggccaccgat cacatcgact
    10861 ggattgccgt gggtggtctg acggcgtcgg cggtcgaggt gccgtcgatc tgggccagtg
    10921 accactttcc cctggtcgcc acgctccact gaaacaacac gtcatttcat atcatttgat
    10981 ctgccttgat cccgtgagga accttcctta ctgtggccgt gcacggcggc agtcgaggag
    11041 cgggatgcag ggcgagggca acagggtggc gacggagatc accgccgata tggtgatcga
    11101 ggtctccgag gtggttcccc gccaccccgc cgacctgacc ggagacctcg tgatcgatct
    11161 gcgcccagag ctcgacgaca ccatcacgga gtcgtcgcgc gccgacatcg ccgatggcgt
    11221 cgacgccgag gctcagacgg ttccggcggc gccggggccc cttcacggcc gcgtcgcggt
    11281 ggtcaccggc ggagccaacg gggtcggtcg agaggtggcg ctcggtctcg ctcgctcggg
    11341 tgcccgcgtc tgtgtgctgg gccgggagct ggcgcagttg cgcgagaccg tcgagctcgc
    11401 cggtcccgaa gcggccatcc tcttcctgca atgcgacgtc ggcagcctga gtgagatcga
    11461 aggggtcgtc gatttcatcg aacgcttcga ccgtccggtc gacatcctcg tccacgctga
    11521 aggggtgcag gtccgaggcg ggatcgagca cggctcggtg aacgacctcg acgagcagta
    11581 cctcgtgaac gtccgcggtc cgtacctgat gtcccagcga ctgctcgccc agctgcgcgc
    11641 cggccggggt cacgtggtgt tcgtgaaccc cgcgccgtcg agcgagggag cgatcgactt
    11701 cggccagttc gccgtcacca ccgacagcgt ccgctcgctc gccgacaccc ttcggcggga
    11761 agtgcagacc tcgggcgttc gggtgacgtc gatccgtccg gaggcgccga ccaccgacgt
    11821 ggcggccgag acggtcgtcg cgctcagccc ccacgacatc gccgacagcg tcctgcacgc
    11881 cgtgcaggtt ccgggtcagg tcgagatcac cgacatcacg ctgcgccccc ggtcagcctg
    11941 agaacgtgcc ggttcatcgg tcgagttcga ccgggacggc ggtagcgtgg ccctcgatgg
    12001 gttggtcgtg ccggcgcccc ggatgtggca gagaagcaga ggtgtgcatc agctacgacg
    12061 ccctcgcgtg tcaggtgtgg ttggatccga tcgcgatggc ccctgccggt ggccagccgc
    12121 tctgcgccga ccacgcgcag cgcctgagcg cacctcgggg atgggtggtc ctcgatcggc
    12181 gcacgtcgca gtcgacgttg atgacggccg ctggtccggc cccggtgcac gatgcccgag
    12241 gccgcgccgc cgttccgcgg cgccgtctgc cgcgggcgtg gggccagttc gacgctcccc
    12301 gcctcgagtt cctcgccgag ggcgacgacg tcgtcaccga accggagccg acgttcgagc
    12361 ctcctgtcgt cgctgcgacg gtgcacctcg aacccgcgct catcgagccc gagccggttg
    12421 tcgagccgga ggccgagccg gagcctgccg ctgtcgtaat tcatgcagaa ggtgacgtct
    12481 cgcgtcgtga tctgcatgga tttccgaccg ccgactcgtc tgagccggag gccgaggctg
    12541 tcgggccaga gccggaaccg gtcgtcgagc cggaacgcgc gcccaccgca gtcgtcgctc
    12601 cggaggccga gcccgtcggg gtcgtaattc atgcagaagg tgacgtctcg cgtcgtgatc
    12661 tgcatggatt tccgaccgcc gactcgtctg agccggagcc cgagctggtc gtcgagccgg
    12721 aggccgagcc catcgcggtc gtcgagcccg agccggtcgc cgagccggag ccagagcccg
    12781 agccggtggt gaagaaggcg ccggctcgcc ggagtcgagc gcgagcggag accggcccgc
    12841 tcggcaagcc caagggtcgc ctcctcagtc gggcgttcga ggcgtcgggt ccgcaacgct
    12901 cggccatcac cgagtcgctc gaggcgctcg gcgacgacga cggttccacg atcgaggccg
    12961 cggagggctg agcccccact accgttgccc ggtgacctca accggtgcgg acactcggat
    13021 cgaccctcgg ctggtcctcc tcctggcggc ggcatgcggt ctcgcagtcg ccaacctcta
    13081 ttacgcgcag ccactcctgc acacgatcgc cgaggacttc ggcaccagtt ccggaaccgc
    13141 tggcctcgtg gtgaccttgt cccaggtcgg ctacgccgtc gggctcgcgc tgatcgtgcc
    13201 ggtcggcgac ctgctcgatc gtcgtcggtt ggttcccgcg attctcgtgg gcacggccgt
    13261 cgcgaccgtg gcggcggcgc ttgccccggg catcgcggtc ctcattgccg tggcggcatt
    13321 catcgggctc gggtcggtcg ccgctcagct cctcgtcccg ctcgccgccg agctcgcctc
    13381 cgaggaggag cgcgggggcg tggtcggccg ggtgatgagc gggttgctgc tgggaatcct
    13441 gctggcccgc acggttgccg ggctcgtcgc cggcgtcgcc ggttggcgtg cggtctactg
    13501 gaccgcggca gtcgttgcga cgggattggc gatcgtgctg gcccgagcgt tgcccgccga
    13561 cgggccccgc ccgtcgaggt cctacggcga cctcctccgc tcgactgccc gcttgttcgt
    13621 gagcgagccg gtgctgcgtc gacgcgccgt gttcggcgcg ttcgggttcg ccgcgttcag
    13681 cgtcttctgg acgaccgtgg ccttcgaact gtcgggtccc cgctacggct acggcgactc
    13741 ggtcatcggg ctgttcggtc tggtgggtgc tgccggtgcg ctgtgtgcag ggtttgccgg
    13801 ccggctcgcc gaccgcggtc gcgccaacat ctcgacggtc gcgttcgccg gttcgatcat
    13861 ggtgtcgttc ctgccgttgt ggtggggtcg gacatcgatc gtctggctcg tcgtcggcat
    13921 cgtggtgctc gacgtcggag tccagggcct ccaggtcacg aaccagagcg tcatctacgg
    13981 gttgcccgtc gctcggagcg gcgtcaactc cgtgtacatg gtgtgctact tcagcggtgg
    14041 cgcactcggc tcggcggtgg ccgccgggtt gtacgactcg ggtgggtggt cgtcgatctg
    14101 cctgctgggc gctgggctcg gagcgctcgt gacggtcgga gcgttgcccg gcctgcggcc
    14161 cacgcccgtc acgcgctgat ccgggtcact cctcggtggc gcgttttcgt cgcttcccct
    14221 cctgcatcgc cgcgacgcgg gcgaccggga tcccgtggcc ctcggcgatc agctggtcgg
    14281 gaaggcgctg ggaagccggg agcgactcga cccagggcgt ccgatgctcc atcagcgcga
    14341 ccgcggtgag gacggtgaag tcgccaggcg agacgtcgtc gagctcgtcc cactcgaggg
    14401 ggaacgacac cggaactccg gggcgggccc gggggctgta ggcggcggcg acggtggcgc
    14461 cgccggaccg ggtggagtcg acgaacacct tcccacgccg gtcggccttg atgtaggcgg
    14521 tggttgcgac gtcggggtcg aggagctcgg cacgacgggc gatggcgcgc gtcgccgccg
    14581 ccacgtcctg ggggtcgtgg tcgggatcga cgggcacgaa gacgtgcacg cctttggctc
    14641 cgctcgtctt caccgcggcg gcgagaccca cgtcgtccag cgcgaggcgc acgagctcgg
    14701 ccgcggccac ggcaccggcg aagccgccct cggtgggcgg gtccaggtcg aggatcagat
    14761 cggtcgcgtg gtgcaacgag ccggcgcgac cgagggtcgg gtgatactcg acggcgcgtt
    14821 ggttggcgaa ccacaggagc gttcgccggt cgttgcacag ggcgtaccgc acggcgcgct
    14881 gtgacgcctc ggcccagatc tcgacggtct cgatccagtc gggggtgtac ttcggcacgt
    14941 tcttctgcat gaacggccgc tggcggtcgg tcgcgcggac gaccgacagc ggtcggtcgc
    15001 ggagcaccgg gatcagtcgc tcgtggacgg tgtccaggta gtcgacgagg tcgcgtttgg
    15061 tcacgccggc gccgtcgaac agcggtcgat cgaggttcgt gagcccgacc ccgtcgcgga
    15121 cctcgtcggg tgtgctcgcc atggtgggcc ctctccggcg tcgggcggac ggcgtcagtg
    15181 ccgccccggt gtcgctgccg cgatcggctg ctcgcacagt gtgacgtgaa cgcgtcatcg
    15241 gtgcgtcggc ggcgatcgcc gggtaggttg tcacgagtac ctggtcgcgg ggccgggttg
    15301 atcgggaggg gagccccaat gcgcaggctg tcgacgttgg ccacactcac ggcggtgctc
    15361 gccctgttcg ctgcggcgtg tacgcccggc gggccggcac cgatcacctg gaaggtgaag
    15421 ccggcgacca tccaggtcat ctcgaagaac agccaggtca cggccggcga ccggccctat
    15481 gtcatccagg tcgggttccg ttcgaagctc ggtgtggccg gctcgtcgag cgcccagatc
    15541 tcgtcgcagt gctcctcggg agcgctgccc gcgcccgacg ccgctccggc tggcaccacg
    15601 gtcggcatcc cggccggcac cgccgacatt cccttcccgg atgcgcagaa cctcgacatc
    15661 ggcgacgtgc tgttgtcgac ggccccgttc gagatcttcg gcaccctcag cttcgcggtc
    15721 aaccgcaacg cgccgccgtt cgtcaccggg tgcgccatga ccgatgcact caacggcgcg
    15781 ttggtcggcg tgctcaagtc gtcgctcgac gtactgatcg ccggctccgc agtgcctccg
    15841 acccaggagc agctgatcga cctcatcgtg tcgaacctcg gcaacttcct gtcggccgcc
    15901 ggcagtttga tcgcagcgat catcgagggc ctcggcaacc ccgacgcgat cgtgggcgtc
    15961 ggcgtgcagc tgctgctgcc cacggcggga gcgttcacgg gtctgctcga caccgcgttc
    16021 ggcgtcgccg gtctgttcag cccggagctg gccaacggct tcatcccgat cccgagcctg
    16081 ccgagcgcaa tcaagatcaa ggtcggtccg ctggccccgt cggtgtcggt gttcgacttc
    16141 gacagcagtg acagcggcgg cgggcactgg gtgtacacga gcacgatcgg cgtctgatcg
    16201 gcgagtcgcc atctatatgc acttgtcaat gtatatagag ggctctatat ttctcgtcga
    16261 tggcgaccca gcagacgcgt cgagccgaca cccgcgtgcg attgctcgcg gctgcggcag
    16321 aactcttcgc cgagcgtggc gtcgacagca cgtcgatcgg cgccatcgct gagctggcag
    16381 accgcacctc cggcgcggtc tatgaccact tcggtggcaa ggacggcgtg ctcgtcgcgt
    16441 tgctcgactc gtgggtcgtc gacctgagcg cggtgatcgg ggcagaactc gccacggcaa
    16501 ccacgctcga ggagcggctg gccgtgctgt ggcgcaacgt gtccgcgccc gccgtcggcg
    16561 acggccggtg gattgccctc gagctcgagc tctggaccca cgcctcgcgc gaccccgccg
    16621 ctcgtgaaca cctcgctcgc cggtaccgcg gcgcctggcg cggcatcgtc gagctggccg
    16681 acgcgtcgaa cgacgtgtcc cccgatgtcg gtcctgccgt catcgggttg ctgttcggcc
    16741 tcgagatgat gcggcgggtc gaccccgtcg cggtcaccga tcggctcgcc atcgactcac
    16801 tcactgctct cgtccaacgg catcgatccg atgccaccac ccggtgaagg gatacaccaa
    16861 ccatgcgtac tcaactctgt gacgacctcg ggatcgagtt cccgatcttc gcgttcaccc
    16921 actgccgcga tgtcgtcgtc gcagtgtcga aggccggcgg cttcggcgtg ctcggcgcgg
    16981 tcgggttctc gcccgagcag ctcgagatcg agctcaactg gatcgacgag cacatcggcg
    17041 atcaccccta cggcgtcgac atcgtcatcc ccaacaagta cgagggcatg aacgaggaca
    17101 tgtcggccga ggacctcacg aagatgctgc aggagatggt tccccagcag cacctcgact
    17161 tcgcccgcaa gctcatgacc gaccacggtg tcccgcttcc cgagggcgag gacgacaacg
    17221 cgttgaagct gctcggctgg accgaggcga ccgccacgcc gcaggtggag atcgcactcc
    17281 gtcacccgaa gatgacgctc atcgccaacg cgctcggcac tccgccgaag gacatgatcg
    17341 accacatcca cgccgagggc cgcaaggtcg cggcgctctg tggctcgccg taccaggcgc
    17401 tgaagcacgc cgacgccgac gtcgacatca tcatcgccca gggcggcgag ggtggcggcc
    17461 actgcggt
//
LOCUS       DAOSOA010000022        17250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3328_length_17250_cov_5.659785, whole genome shotgun sequence.
ACCESSION   DAOSOA010000022 DAOSOA010000000
VERSION     DAOSOA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17250
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_17250_cov_5.659785"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKY13_02930"
     CDS             complement(<1..233)
                     /locus_tag="PKY13_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442621.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT78910.1"
                     /translation="MDAFGEYDVPKGRVLVAKLGLDGHDRGVKVVARILRDAGFEVIY
                     TGLRQTPAKVVAAAVDEDVDAIGLSMLSGAHLAL"
     gene            complement(247..1893)
                     /locus_tag="PKY13_02935"
     CDS             complement(247..1893)
                     /locus_tag="PKY13_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOT78911.1"
                     /translation="MDAPRPSSRSSWEAAFASSPTRDAEFETMSGIPIEPVYGPDDAE
                     HPGEYPYTRGPYASMYRSKLWTMRMFAGFGTAIDTNGRFKDILANGGDGLSTAFDLPT
                     LMGCDSDDEMSLGEVGTCGVAVDTQADMEDLFRDIDLEHTTTSMTINSPAAPIFAMFI
                     AAAEKAGMSRAKLGGTLQNDILKEYQAQKEFIFPPRPSLRLVRDTMAFCAAEMPRWHA
                     ISVSGYHIREAGSTAAQELAFTLGNGFAYVELGLKAGLKVDDFAPRLSFFFNAHLDFF
                     EEIAKYRAARRIWARWMQERYGAQAAKSLQLRFHTQTAGVSLTAQQPEVNIVRTAIEA
                     LAGVLGGTQSLHTNSMDETLALPTEKSARIALRTQQVIAHETRVTNVADPLGGSYFVE
                     ALTDEIERQAEELFAKVDAFGDGSMLEGAIHGIEEGWFQGEIAQAAYDLEKKLNDGRH
                     VVVGVNQFFEGNDEPQPETLYIDREVEQFQIKRLDAVRHDRNAAAVDEVLTRLRADAA
                     DPEVNLMPVLMEASRAYCTLGEMMGAMADVFGRWEETARI"
     gene            1968..2204
                     /locus_tag="PKY13_02940"
     CDS             1968..2204
                     /locus_tag="PKY13_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78912.1"
                     /translation="MHEADETSSSLPETIVCVDCGGTCHRTSLPLDGGDETGDIVTYR
                     CEDCHDRWDLIAGDPADLDDDGNSLLDDHRGHLH"
     gene            2204..3139
                     /gene="pip"
                     /locus_tag="PKY13_02945"
     CDS             2204..3139
                     /gene="pip"
                     /locus_tag="PKY13_02945"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HOT78913.1"
                     /translation="MAELYPEIEPNATYSLDVGDGHVLYVEESGNPDGKPAVVVHGGP
                     GGGSAPKQRRFFDPDAYRIVLLDQRGCGRSTPTASVEHNTTWHLVADLERVRALLGID
                     RWLVFGGSWGSTLSLAYSQMNPERVTELVLRGIFLLRRSELDFFYQHGTSDLFPDAWE
                     QYLAPIPDEERGDLVAAYHRRLFGDDPEAQLECARAWADWERATAYLEQRPGTDSDQE
                     MLNLARIENHYFVNRGFFTHETQLLDGVDLIRHIPAVIVQGRYDCVCPMRTAWDLHRA
                     WPEAEFVLNQRAGHSMFDPENTSALVDAVDRFRAV"
     gene            complement(3143..3589)
                     /locus_tag="PKY13_02950"
     CDS             complement(3143..3589)
                     /locus_tag="PKY13_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HOT78914.1"
                     /translation="MTETDAQPDAEHEWWKTFVDESPARTAKLAVVRADGSPQVAPVW
                     IARDGDEIVFLTSADTVKGKSILRDGRVSLCLDNELPPFDFVTINGTTTTSTDPDELL
                     YWATESARRYMGDELAEQYGRRNAVPPEMVVRVTPTRVIAARNVSD"
     gene            complement(3650..4852)
                     /locus_tag="PKY13_02955"
     CDS             complement(3650..4852)
                     /locus_tag="PKY13_02955"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HOT78915.1"
                     /translation="MAGVRGRTSRWAWALGVALAGATLAGCVPDIGGGGGPTTTEPTV
                     VVGDDQIRLNQIQLVGSHNSYHIAPEGTILIPLTLGGLAVPGLVEGLGNPLSLNYTHA
                     PLPTQLQRGVRTFELDVYADPLGGRFSVPRLVELFNVQQPNIPPGLDQPGLKVLHIAD
                     VDFLTTCVTLQACLGQIRTWSDAHPDHLPIVIDVELKGEGLGLPAELGFTPIVPFDGP
                     QLDAVDAELRAAIGDRLITPDDVRQGASTLREAITTTGWPTVAESRGKVLVFLDNEGL
                     RSSYLSGHPSLEGRAMFTSSVPGQPDAAIIKENTPNDGATIRGLVEQGYIVRTRADDD
                     TVPDPSVARREIALASGAQIVHTDYPPGEPRFNTGYQVTFGTKVASRCNPVTTTAGTC
                     TAAAVIEP"
     gene            5008..5709
                     /locus_tag="PKY13_02960"
     CDS             5008..5709
                     /locus_tag="PKY13_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOT78916.1"
                     /translation="MTDSTAPATLPDLPGGPYDIEFFFDPGCPFAWQTSQWVRRLVEL
                     KDIKVGWRFISLKFINENNENMPAGYLEAAEKGLRYHRICAAARDRLGNDAVSRLYQA
                     YGERYWYHTSDLDFGERMAEAAGRVDALEILQELDLPVDLADAADDSTWDTLIRAESD
                     AAFERTGPDVGTPILTFDPPHGNSLFGPVISAVPDDETSVKFYEALRTFADFPEFSEL
                     KRTKRAPLDLPLLQF"
     gene            complement(5660..6541)
                     /locus_tag="PKY13_02965"
     CDS             complement(5660..6541)
                     /locus_tag="PKY13_02965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT78917.1"
                     /translation="MRHTITGQRREDRTRRTVAVGTLAAVTPILAPAATIWASWDEAG
                     IAVVTMLSTVGAASIVGLLLLRVTRRLARQQALFDEERRRWRDEANTDPLCRIPNRRG
                     ADDQIAGMLRAAPADSRWTVLALDVDHFKQVNDTHGHAVGDRVLASVASALSDHLPPG
                     TAAARWGGDEFVVFGLVDQELPQGWAEELVAAVTARPVQCREGNLRVGVSFGLAHGPS
                     GQPFDDILDLADDALLRAKTVLRAEGRATIRLDDREVVTVATRPPAIPAATVSDPGGV
                     AVRTAAVAGPAAPAWCA"
     gene            complement(6706..7614)
                     /locus_tag="PKY13_02970"
     CDS             complement(6706..7614)
                     /locus_tag="PKY13_02970"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT78918.1"
                     /translation="MGRALNGEIEIEWEERGPSNGEPILLVMGLGAQMIAWRDGFCDR
                     LVDHGYRVIRFDNRDVGLSSTTAATPPSFGEMATLFGPRPARRPVPYTLSDMASDAAA
                     VLDDAGVEAAHVVGASLGGMIAQTLAIERPERLRSLTSIMSKTGALHVGLPTPRVLRK
                     VMQPTPVERDLALAYELERAEVTCGPLFDRDAMETFLAEAYERSPERGGMAFQLSAMF
                     ASGDRTRALRNLDVPTLVIHGRADGLVRLSGGVATARAIPGAALEVHNLMGHDLPQPL
                     WPALTDSIAAHARRASRARSRDLTPA"
     gene            complement(7626..8843)
                     /locus_tag="PKY13_02975"
     CDS             complement(7626..8843)
                     /locus_tag="PKY13_02975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT78919.1"
                     /translation="MTTDSKTSNVVRAGGVAVFALAILGAGAAIGLSTADAEPTSAPL
                     ATSPLGAAPLPTMSPLWVIVVAVAEGVLLTIGTLLVLSVVRDVRRRRAVVTALPTVES
                     DLERALRAIAEAPDRSAALVEMATGAFESTNALTVAVVVRSGRGALEVATLSGEPVGS
                     DALLPDGVSTAIDQVAPRRWVDTGSDDGSVAPRAYIVAPVVIDGSAIGALVGTRHGAT
                     AFGIADLDAVCQLAAVELPAEPALVDDLTDLPTRRRLDHDLGLVADGQVGLAVVAIDH
                     FLRIGHEVGPAAIDGLVRELVDVVAGAVRDGDLVYRYGDGQFGVLLPSAGKDETAEVA
                     ERLRVAVEAHEFGLDEDATARRITVSVGTVVADADALADITRRADDAVSEAERFGRNR
                     VVVSGHARFPVDR"
     gene            complement(8893..10434)
                     /locus_tag="PKY13_02980"
     CDS             complement(8893..10434)
                     /locus_tag="PKY13_02980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOT78920.1"
                     /translation="MGLGPTAHAAPPDTTDALVAVESEVLALVELGAFREPLPAKLRD
                     VAADWRSEVEGYTRRDLIDWLQASDEDAAHELDLLGGVPLSPDVRAALRRLPPDAVRA
                     IESGTPIAMPASVYEHALNDLEHRNGSAPSAEEAAPAAGGGVAPRPEPRADGTSLVLV
                     LGAGAAVVVAALGAAMVVWGRRRSTAVTATDSGVARLLDASRRFGSSVAVPDIHRILL
                     EETVALTGADTAAVVSVEDGSSLPTVVTETTVGSLVPGHLGRGIVRRVLDTGVGIRTV
                     VTDAAIAGGPSAVLAVPLVRNGTIVGAVVARRGVEQPFDPADLDLLGQLAPMAAAALV
                     SAGLHDNVAELSLTDGLTALGNRRRLDRDLVAALGAGSGNIGFVMVDIDHFKQFNDTH
                     GHVAGDDVLQGVAALLRGAVRAGDVVYRYGGEEFCVLLPDTSPVEAADVSERLRLAVE
                     THDFPGGETQPAGRVTISVGTTVASRIDPTEVKERADHALYEAKHRGRNQVVVESIVV
                     TPVDE"
     gene            10675..11802
                     /locus_tag="PKY13_02985"
     CDS             10675..11802
                     /locus_tag="PKY13_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78921.1"
                     /translation="MELNPRRIVGGLNPLRVGVALMLVGGSTLGLGLVQTTTASMQPS
                     CPGGTQLIAKFEWSGRHYDFDGPSGNGSVVNIGNDRDDGGTWTSTKPVSHLIVKGGPG
                     SVTATFTPPASSGTFSNAGLPKVGGGNTPDISNVQFCGAIPPATTTTSTTTTVKPTTT
                     KATTTTKATTTTAAPTTTKATTTTKATTTTAAPTTTKATTTTTATTTTAAPTTTKATT
                     TTKATTTTAAPTTTKATTTTAAPTTTTTSTTTTSTTTTSTTTTTAAPTTTTSTTTTTA
                     APTTTVAGATSVPSSTSSSTSSTSTSTTSTSTTISPSEVRSETSISKDDNVRVLADGT
                     VQPLAVTGNTSLPLVIVGIVLLAAGAAMTVVTRLRTRRTDA"
     gene            11888..14059
                     /locus_tag="PKY13_02990"
     CDS             11888..14059
                     /locus_tag="PKY13_02990"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT78922.1"
                     /translation="MVLSLAVAAAIVATSDEHRLLAVLLAAATVWSAAPLVVLLLSGT
                     GTVGATDPTPAGPTPTGPPSAAFTTIVRVGDEPLDIARTTILLAAAAGPTIVLTGPGA
                     PPQLHRLGVPVIEDADAAAGLVRAVARVDTSAILLVSGRAVPVADACRRGAARLEPGI
                     GWVTGRTAPFSRDRYVGDSRHDVTVALVAAARRRGLVAWEPDATIIATELLRSHPLDA
                     DRPLGSWLRRRAGEGWGGAALDEPIARRAAPVDAAAYWPDTIARQWGRTADLAAAIGT
                     GSTRCRLLAAGLTLRELFAVPLLFASALPLFAAGPTTPSTTTVMVASVAVAAAGIRWA
                     ALRQGLGLPMRPLRDVTAAIHSTPGSLVAVGAAVTRSRRAPRLALPERPLVWAALLLT
                     VVTGARLVDGTTARPSAPVMGTALAALAALWAFSMGALAQRNWARSTYRVPLDVGLVS
                     AGTTFRTVNGSPSGLALVRLCDGPDVDATASRLAVSIALDDGTAIDASARVSGRRRSG
                     DREVIGLVLNLGDRARAAWVDQLDRAAQGSGPERAGARVDALTRSPAAPDDGPRRGRA
                     ARVLDGAGAALAAIVSLVVVAALVLVLFGFHPLVIRSASMTPTYAIGDVVVSENVLAG
                     DLRPGDVATVPADAIGETLTHRVVSVAGVGSSVTVETRGDANTTGERVVLDAGTTVGR
                     VRWSVPWLGRPANSLRSSPVRVALVGAAAALLITAVRRRVR"
     gene            14105..15121
                     /locus_tag="PKY13_02995"
     CDS             14105..15121
                     /locus_tag="PKY13_02995"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT78923.1"
                     /translation="MFPTRFDSILDLARLPWFEVQGGNRLVADPQVGPVIDMHTHLAM
                     AYLARHRVDVLAETARTAYYLPLTMPFDLSPYSNLNFDAESMFAMKADLSVLSLTPWG
                     KRVTHTAPNLLRDMDDLGIRDAVLLPFDLPVGPSNADVFLAAARRHPRLHSFAGVHPS
                     DRRLEQRLDEQIAAGAAGLKFHPNGQFMAADHPKTVHLCRRCGDRGLPVLMHCGPVGI
                     EPKAAERRSQVARYEQAIAENPDTTFILGHCGALQHEVALDSFVGRYDNLYWELSSLG
                     LDAMRTVFDRVAPDRLLYGTDWPFYHQAITMARVLILAEDNDALRRAVLHDNAARLLG
                     LDSA"
     gene            complement(15178..16194)
                     /locus_tag="PKY13_03000"
     CDS             complement(15178..16194)
                     /locus_tag="PKY13_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78924.1"
                     /translation="MDAERRPSRQRRWPMVLIGLATAGVLVAAGVFVATREPTVPVTE
                     RATPPTTTLRPCVQKPAAVEGTEGAAEPPDGTYLVLISPVIGVAAVDVAVVEGGTALE
                     FSDVPVADDVSITGQGPAAITVRNGSIVGFDPLHATWDDPLGRGGDPCTPDTECELSE
                     DPIPHLWPGGIDGLLGETLAEVPNQLAMALAKWGVDVDPASFALSSERCGFSATVGGD
                     LTLQLYLGLTDDGYVLSGATTDQPEDDVAGVGYTADGETVQLSLASVFRDRYPEATVD
                     VVVRFGERSEKATAPVADETIAVTIDRRLSQRGSYVLKYTNPDGSIRRIAAAQLPAGN
                     SAAG"
     gene            complement(16459..16532)
                     /locus_tag="PKY13_03005"
     tRNA            complement(16459..16532)
                     /locus_tag="PKY13_03005"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16496..16498),aa:Pro,seq:ggg)
     gene            complement(16677..>17250)
                     /locus_tag="PKY13_03010"
     CDS             complement(16677..>17250)
                     /locus_tag="PKY13_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT78925.1"
                     /translation="HEVVAAVGDGPSFVEAVLAERPDVALVDVRMPPSHSDEGLRASV
                     QVRSQWPEAKVMVLSQYVEVSYADELLGTGNGGVGYLLKDRVSAIDEFLDSVERVAAG
                     GTVLDPQVVSQLMVRRRGDDPVTTLTPRETEVLGLMAEGRSNASIAQALVVSDGAVEK
                     HTQRIFAKLGLPPDEGQHRRVLAVLAYLRS"
BASE COUNT         2592 a         6214 c         5945 g         2499 t
ORIGIN      
        1 agcgcgaggt gggcgcccga gagcatcgag aggccgatgg cgtcgacatc ctcgtcgacc
       61 gccgcggcca cgaccttggc cggcgtctgc cggagcccgg tgtagatcac ctcgaagccg
      121 gcgtcgcgga ggatgcgcgc cacgaccttc acaccgcggt cgtggccgtc gagccccaac
      181 ttcgcaacca ggacccgccc tttcgggacg tcgtactcac caaaagcgtc catcagcagt
      241 acgtcctcag atccgagctg tttcttccca gcggccgaac acgtcggcca tggcacccat
      301 catctcgccg agcgtgcagt aggcccgcga tgcctccatg aggacgggca tcaggttgac
      361 ctcggggtca gcggcgtcgg cccgcaggcg ggtcaggacc tcgtccacgg cggcggcgtt
      421 gcgatcgtgg cgcacggcgt cgagccgctt gatctggaac tgctcgacct cgcggtcgat
      481 gtagagcgtc tcgggctgcg gctcgtcgtt gccctcgaag aactggttga ccccgacgac
      541 gacgtggcgg ccgtcgttga gcttcttctc gaggtcgtac gcagcctgag cgatctcgcc
      601 ctggaaccag ccttcttcga tgccgtggat cgcgccctcg agcatcgatc cgtcgccgaa
      661 ggcgtcgacc ttggcgaaca gctcctcggc ctgccgctcg atctcgtcgg tgagcgcctc
      721 gacgaagtac gagccgccga gcgggtcggc aacgttggtg acccgggtct cgtgggcgat
      781 cacctgttgg gtccgcaggg cgatgcgtgc cgacttctcg gtggggagcg ccagggtctc
      841 gtccatcgag ttggtgtgca gactctgcgt gccgccgagc accccggcga gcgcttcgat
      901 ggcggtgcga acgatgttga cctcgggctg ctgggcagtg agtgacacac cggccgtctg
      961 ggtgtggaat cgcagctgga gagacttcgc ggcctgggcg ccgtagcgct cctgcatcca
     1021 ccgggcccag atgcggcggg cggcacggta cttggcgatc tcctcgaaga agtcgaggtg
     1081 cgcgttgaag aagaagctga gccgcggcgc gaagtcgtcg accttcagcc cggccttcag
     1141 acccaactcg acgtaggcga acccgttgcc gagggtgaac gccagctcct gcgccgcggt
     1201 cgaaccggcc tcacggatgt ggtagcccga caccgagatc gcgtgccacc gcggcatctc
     1261 cgcagcgcag aacgccatgg tgtcgcgcac gaggcggagc gaggggcgcg gcgggaagat
     1321 gaactccttc tgcgcctggt actccttcag gatgtcgttc tggagggtgc caccgagctt
     1381 cgcccgcgac atgcccgcct tctccgccgc ggcgatgaac atggcgaaga tcggcgcggc
     1441 cggcgagttg atcgtcatcg acgtggtggt gtgctcgagg tcgatgtcgc ggaagagatc
     1501 ctccatgtcg gcctgcgtgt cgacagcgac gccgcaggtt ccgacctcgc cgaggctcat
     1561 ctcgtcgtcg gagtcgcagc ccatcagggt gggcaggtcg aacgcggtcg acaggccgtc
     1621 cccgccgttg gccaggatgt ccttgaagcg cccgttggtg tcgatggcgg tgccgaaccc
     1681 ggcgaacatc cgcatcgtcc acagcttcga gcggtacatc gaggcgtagg gcccgcgggt
     1741 gtaggggtac tcccccgggt gctcggcgtc gtcggggccg tacaccggct cgatggggat
     1801 gcccgacatc gtctcgaact cggcgtcacg ggtgggcgac gaggcgaacg ctgcctccca
     1861 gctggaccgc gatgagggtc ggggtgcatc catggggacc acgctaccgc tcctcgcccc
     1921 gagggctcac gaccggatcg gccggggcgg tgcgcgagac tgcgggcatg cacgaggccg
     1981 acgagaccag cagttcgctc cccgagacga tcgtgtgcgt cgactgcggc ggcacctgcc
     2041 accgcacctc cctgccgctg gacgggggcg acgagacggg cgacatcgtc acgtaccgct
     2101 gcgaggactg ccacgaccgc tgggacctca tcgccggcga ccccgccgac ctcgacgacg
     2161 acggcaacag cctcctcgac gaccaccgcg ggcacctgca ctgatggctg agctctaccc
     2221 cgagatcgaa ccgaacgcga cgtactcgct cgacgtcggc gacggccacg tcctctacgt
     2281 cgaggagtcg gggaaccccg acggcaagcc cgcggtcgtg gtgcacggtg ggcccggcgg
     2341 cggctcggcc ccgaagcagc ggcggttctt cgaccccgac gcgtaccgca tcgtgctgct
     2401 cgatcagcgc gggtgcggcc gcagcactcc caccgcatcg gtcgagcaca acacgacgtg
     2461 gcacctcgtc gccgacctcg aacgcgttcg cgcgctcctg gggatcgacc gctggctcgt
     2521 gttcggtggt tcgtggggct cgaccctgtc gctcgcctac tcgcagatga accccgagcg
     2581 ggtcaccgag ctggtgttgc gtggcatctt cctgctccgc cgcagcgagc tcgacttctt
     2641 ctaccaacac ggcacatccg acctgttccc cgacgcatgg gagcagtacc tggcgccgat
     2701 ccccgacgag gagcgcggcg atctcgtcgc ggcctaccac cggcgcctgt tcggcgacga
     2761 tcccgaggcg cagctcgagt gcgcccgggc atgggccgac tgggagcggg ccaccgccta
     2821 cctcgagcag cggccgggca ccgactccga ccaggagatg ctcaacctcg cccgcatcga
     2881 gaaccactac ttcgtgaacc gtggcttctt cacccacgag acccagctgc tcgacggcgt
     2941 cgacctgatc cggcacatcc ccgcggtcat cgtgcagggc cgatacgact gcgtgtgccc
     3001 catgaggacc gcgtgggacc tccaccgggc ctggccggag gcggagttcg tgctcaacca
     3061 gcgcgccggc cactcgatgt tcgaccccga gaacacgtcc gccctcgtcg acgcggttga
     3121 ccggttccgc gccgtctgag cgtcagtcgc tcacgttcct cgccgcgatg acccgggtcg
     3181 gggtgacccg gacgaccatc tcgggtggca cggcgttgcg ccggccgtac tgctcggcga
     3241 gctcgtcgcc catgtagcgc cgtgccgact cggtggccca gtacaacagc tcgtcgggat
     3301 cggtcgacgt cgtcgtcgtg ccgttgatgg tgacgaagtc gaacggcggc agttcgttgt
     3361 cgaggcacag gctcacccgt ccgtcccgca ggatcgactt gcccttgacg gtgtcggcgc
     3421 tggtgaggaa cacgatctcg tcgccgtcgc gggcgatcca caccggcgcg acctgtggcg
     3481 acccgtcagc tcgaacgacg gcgagcttgg cggtacgggc cggtgactcg tcgacgaacg
     3541 tcttccacca ctcgtgctcg gcatcgggct gggcatcggt ctcggtcatg ccgggcacaa
     3601 ccctcgacgg ggcgcgggct attccgccgc gacccgtcgg ggtgcgggat cagggttcga
     3661 tgaccgccgc agcggtgcag gttcccgcgg tcgtcgtcac cgggttgcag cgcgaggcga
     3721 ccttcgtgcc gaacgtgacc tggtagccgg tgttgaacct cggctccccg ggtgggtagt
     3781 cggtgtgaac gatctgcgct cccgacgcga gggcgatctc ccgacgggcc accgacgggt
     3841 cgggaaccgt gtcgtcgtcg gcacgggtcc gcacgatgta gccctgctcg accaggcctc
     3901 ggatcgtcgc gccgtcgttg ggcgtgttct ccttgatgat cgccgcgtcg ggttggcccg
     3961 gcaccgacga cgtgaacatg gcgcgtccct cgagcgacgg gtggcccgac aggtaggacg
     4021 agcgcaggcc ttcgttgtcc aggaacacga gcaccttgcc gcgcgactcg gcgacggtcg
     4081 gccaccccgt cgtcgtgatc gcctcgcgca acgtggacgc gccctggcgg acgtcgtcgg
     4141 gggtgatgag gcggtcaccg atcgcggcgc gcagttcggc gtcgacggcg tcgagctggg
     4201 ggccgtcgaa cggcacgatg ggggtgaacc cgagttcggc ggggagtccg agaccctcgc
     4261 ccttcagctc gacgtcgatg acgatcggca gatggtcggg gtgagcgtcg gaccaggtgc
     4321 ggatctgtcc gaggcacgcc tgcagggtga cgcaggtcgt gaggaagtcg acgtcggcga
     4381 tgtgcagcac cttcaacccg ggttggtcga gccccggcgg gatgttcggc tgctgcacgt
     4441 tgaacagctc gacgaggcgt gggacggaga accggccgcc gagcgggtcg gcgtagacgt
     4501 cgagttcgaa cgttcggacg ccgcgttgca gctgcgtcgg cagcggcgcg tgcgtgtagt
     4561 tgagcgacag cgggtttccg aggccctcga ccagaccggg cacggccagg cccccgagcg
     4621 tgagcgggat gaggatcgtg ccctcggggg cgatgtggta gctgttgtgc gacccgacga
     4681 gctggatctg gttgagccgg atctggtcgt cccccaccac gaccgtgggc tcggtcgtcg
     4741 tcgggccgcc tcccccgccg atgtcgggca cacatccggc cagggtcgcc cccgccaacg
     4801 cgacgccgag cgcccatgcc caacgagacg tcctaccgcg cactcctgcc atgtggcctc
     4861 cccgcccatc gcactccccg ggccccatcg tcgcagccga ttgaacacga ggcaaccgat
     4921 cggggaacac ggagtgacac tcaggtgacg tacgggccgc gacctgctgg cgggaacatc
     4981 gctccgccgg tgggcgttgc aggcaccatg accgattcca ccgcgcccgc aacgcttccc
     5041 gacctccccg gcggccccta cgacatcgag ttcttcttcg atccgggctg tccgttcgcc
     5101 tggcagacct cgcagtgggt ccgccgcctg gtggagctga aggacatcaa ggtcggctgg
     5161 cgattcatca gtctgaagtt catcaacgag aacaacgaga acatgcccgc cggatacctc
     5221 gaagcggccg agaagggcct gcgctaccac cggatctgcg ccgcggcgcg tgaccggctg
     5281 ggcaacgacg ccgtgtcccg cctctaccag gcgtacggcg agcgctactg gtaccacacg
     5341 tcggatctcg acttcggcga gcgcatggcc gaggcggccg ggcgcgtcga cgcgctcgag
     5401 atcctgcagg agctggacct gccggtcgac ctggccgacg cggccgacga ctcgacctgg
     5461 gacacgctca tccgcgccga gagcgacgct gccttcgagc ggaccggtcc cgacgtcggc
     5521 acgccgatcc tcacgttcga cccgccgcac ggcaactcgc tgttcggtcc ggtcatcagc
     5581 gccgtgcccg acgacgagac ctcggtgaag ttctacgagg cgctccggac cttcgcagac
     5641 ttccccgagt tctccgagct caagcgcacc aagcgggcgc cgctggacct gccactgctg
     5701 cagttctgac cgccacgccg ccgggatcgg acacggtcgc ggcgggaatc gccggcggtc
     5761 gggtcgccac cgtgacgacc tcgcggtcgt cgagtcggat ggtcgcccga ccctcggccc
     5821 ggagcacggt cttggcccgc aggagcgcgt cgtcggccag gtcgaggatg tcgtcgaacg
     5881 gctggcccga ggggccgtgc gcgagcccga agctgacgcc gacccggagg ttgccttcgc
     5941 ggcactgcac gggccgagcg gtcacggccg ccaccagctc ctcggcccac ccctgcggga
     6001 gctcctgatc gacgagcccg aacaccacga actcgtcacc gccccagcgg gcggcggcgg
     6061 tacccggcgg gaggtggtcg gacagcgccg acgccaccga tgccagcaca cggtcaccga
     6121 ccgcgtgacc gtgggtgtcg ttgacctgct tgaagtggtc gacgtcgagc gccagcaccg
     6181 tccaccggga atcggccggt gcggcccgga gcatcccggc gatctggtcg tcggcgccgc
     6241 gacggttggg gatgcgacac agggggtcgg tgttggcctc gtcgcgccag cgtcggcgtt
     6301 cctcatcgaa caacgcctgt tggcgggcga gacggcgggt gacgcggagg aggagcagcc
     6361 cgacgatgct ggccgctccg acggtgctga gcatggtgac gacggcgatc ccggcctcgt
     6421 cccacgacgc ccagatcgtg gcggcggggg ccagaatggg ggtgacggcg gcgagggtgc
     6481 cgacggcgac ggtgcgtcgc gtccggtctt ccctgcgctg cccggtgatg gtgtgtctca
     6541 cgtcgccagt cctccgtgtg tcgctatgga tacgaagtgg gccccagttc gagcggtcaa
     6601 ccgctcatgg tggcacctta cccacggact gtggtactga caccgggtgc tgtaagaaac
     6661 ccctcagccg ggggaaggct ccccgaacgt gacgtcggtc gacggtcagg ccggcgtcag
     6721 gtcgcgagac cgcgcccgac tcgcccggcg cgcgtgcgcg gcgatcgagt cggtcagcgc
     6781 cggccagagc ggctgtggca gatcgtggcc catgaggttg tgcacctcga gcgcggcgcc
     6841 ggggatcgca cgggcggtcg ccacacctcc gctgagtcgg acgagcccgt cggcacgacc
     6901 gtggatcacg agcgtcggca catcgaggtt gcgcagggcc cgcgtgcgat cgcccgacgc
     6961 gaacatggcg ctcagctgga acgccatgcc cccacgctcg ggtgaccgct cgtaggcctc
     7021 ggccaggaac gtctccatgg cgtcgcggtc gaagagcggc ccgcaggtca cctcggcccg
     7081 ttcgagctcg taggcgagcg cgaggtcgcg ctcgacggga gtcggctgca tcacctttcg
     7141 caggacgcgc ggcgtgggga gccccacgtg caacgcaccg gtcttcgaca tgatcgaggt
     7201 cagcgatcgg agccgttcgg gccgttcgat ggcaagggtc tgggcgatca tgccgcccaa
     7261 cgaagcaccc acgacgtgcg ctgcctcgac accggcgtcg tcgagcaccg cggcggcgtc
     7321 cgatgccatg tccgacaacg tgtacggcac gggccgacgc gccggacgcg gaccgaacag
     7381 cgtcgccatc tcgccgaacg acgggggcgt cgccgcagtc gtcgacgaca agccgacgtc
     7441 gcggttgtcg aagcggatca cgcgataccc gtggtcgacg agacggtcgc agaacccgtc
     7501 gcgccaggcg atcatctgag cgcccagccc catcacgaga aggatcggtt cgccgttcga
     7561 cgggccccgc tcctcccatt cgatctcgat ctcaccgttc agcgcacgcc ccatggcgca
     7621 ccacgctacc ggtcgaccgg gaatcgggcg tggcccgaca ccacgacccg gttgcgcccg
     7681 aaccgttccg cctcgctcac ggcgtcgtcg gcgcgacggg tgatgtcggc gagcgcatcg
     7741 gcatcggcga cgaccgtgcc gaccgacacc gtgatccggc gggccgtcgc gtcctcgtcg
     7801 aggccgaact cgtgtgcctc gacggccacc cggagccgct ccgcgacctc ggcggtctcg
     7861 tccttgcccg ccgacgggag aaggacgccg aactgcccgt cgccgtagcg gtacaccagg
     7921 tccccgtcgc gcaccgcgcc ggcgaccacg tcgacgagtt cgcgcaccag gccgtcgatc
     7981 gcggcgggcc cgacctcgtg cccgatccgc aggaagtggt cgatcgcgac cacggccagt
     8041 cccacctgcc cgtcggcaac gaggccgagg tcgtggtcga gacgccggcg ggtcggcagg
     8101 tcggtgaggt cgtcgacgag cgccggttcg gccggcagtt cgacggcggc gagttggcag
     8161 acggcgtcga ggtcggcgat gccgaaggcg gtcgcgccgt gccgcgtgcc gaccagcgcg
     8221 ccgatcgcag agccgtcgat cacgaccggg gccacgatgt aggcgcgggg agcgacggat
     8281 ccgtcgtcgg aaccggtgtc gacccagcgg cgaggtgcga cctggtcgat cgccgtggac
     8341 acgccgtcgg ggagcagcgc gtccgacccg accggctcgc cggacagggt ggcgacttcg
     8401 agggcgcccc gacccgaccg gacgaccacg gccacagtga gggcgttcgt cgactcgaac
     8461 gcaccggtgg ccatctccac gagggcagcc gaccggtcgg gggcttcggc aatcgcgcgg
     8521 agggcccgtt cgagatcgga ctcgaccgtc ggcagcgctg tgaccacggc ccggcggcgc
     8581 cggacgtcgc ggacgaccga caacacgagc aacgtaccga tggtgagcag cacaccttcg
     8641 gccacggcga ccacgatcac ccagagcggc gacatggtcg gcagcggtgc cgcaccgagc
     8701 ggcgacgtgg cgagcggcgc cgaggtgggc tcggcgtcag cggtggagag cccgatcgca
     8761 gcgccggccc ccaggatggc cagcgcgaac acggcgaccc cgccggcccg aaccacgttg
     8821 gaggtcttcg aatcggtcgt caccgtggag gtatcgaccg acgacgccgt cgtcctgaga
     8881 attcggacga ggtcactcgt cgacgggcgt gacgacgatc gactcgacga cgacctggtt
     8941 gcggccacgg tgcttcgcct cgtacagcgc gtgatcggcc cgttccttga cctcggtcgg
     9001 gtcgatccgg ctcgccacgg tcgttcccac cgagatcgtg acccggcccg ccggctgcgt
     9061 ctcgccgccc gggaaatcgt gggtctcgac ggcgaggcgg agacgttcgg acacgtcggc
     9121 cgcctcgacg ggactggtgt cgggcagcag cacgcagaac tcctccccgc cgtagcggta
     9181 cacgacgtct ccggcgcgca ccgcgcctcg caggagggcg gcgacgccct gcagcacgtc
     9241 gtcaccggcc acgtggccgt gggtgtcgtt gaactgcttg aagtggtcga tgtccaccat
     9301 cacgaagccg atgttgcccg accccgcacc cagcgcagcg accagatcgc ggtcgaggcg
     9361 acgccggttg cccagtgcgg tcaggccgtc ggtgagcgac agttcggcga cgttgtcgtg
     9421 cagcccggcg gacacgagcg cggctgcggc catcggggcg agttgaccga gtagatcgag
     9481 atccgccggg tcgaacggtt gctcgacgcc gcgccgggcc accacggccc cgacgatggt
     9541 cccgttgcgc accaacggaa cggcgaggac ggcgctgggg ccgccggcga tcgctgcgtc
     9601 ggtgacgacg gtccggattc cgactccggt gtcgagcacc cgccggacga tgccgcggcc
     9661 caggtggccg gggacgagcg atccgaccgt cgtctcggtc acgacggtcg ggagtgatga
     9721 gccgtcctcc acggacacga cggcggcggt gtcggcgccg gtgagtgcga cggtctcctc
     9781 caacaggatg cgatggatgt cggggaccgc caccgacgat ccgaaccggc gactggcatc
     9841 gaggaggcga gcgacgcccg agtcggtggc ggtcacggca gtggatcgcc gtcgccccca
     9901 cacgaccatg gcggcaccga gcgccgcgac cacaacggcg gcacccgctc cgaggacgag
     9961 gacgagggat gtgccgtcgg cccgaggctc gggtcgtggg gcaactcccc cacccgctgc
    10021 cggcgcggcc tcctcggcgg agggcgcgct gccgttgcgg tgttcgagat cgttgagcgc
    10081 gtgctcgtac accgaggcgg gcatcgcgat gggcgtgccc gactcgatcg cccggacggc
    10141 gtcgggagga agacgtcgta gcgcggcgcg gacatcgggg ctgagcggga cgccgcccag
    10201 caggtccagc tcgtgggcgg cgtcctcgtc ggaggcctgg agccagtcga tcaagtcgcg
    10261 gcgggtgtac ccctcgacct ccgatcgcca gtcggcggcg acgtcacgca gcttcgccgg
    10321 cagtggctca cggaacgcac cgagctcgac gagtgcgagc acctcggact cgacggcgac
    10381 gagcgcgtcg gtcgtgtccg gcggcgctgc atgggccgtt ggacccagtc ccaccccaat
    10441 ggccacgacc gcgagcacca gcgcggcgct cctcccgatt tgaccccatg tcgtcgttct
    10501 ccgcacgagg caggtatcgg atcgtcgggg ggaggacctg agaaggtcag gtcccgggac
    10561 ccatcgaccg gagcgaacga agaagttctc aacagcggcc gaaccgggcc gatacgggtt
    10621 gggtgatttc acccagccat gggtgggggc gcgaactcga ctccggagga catcatggaa
    10681 ctcaaccctc gccgcatcgt cggtggcctc aacccgctgc gggtcggtgt cgcgttgatg
    10741 ctcgtcggcg gctccaccct ggggctggga ttggtccaga cgaccaccgc atcgatgcag
    10801 ccgagctgcc cgggcggaac ccagctcatc gccaagttcg agtggtcggg ccggcactac
    10861 gacttcgacg gcccctccgg caacggcagc gtcgtgaaca tcggcaatga ccgcgacgac
    10921 gggggcacct ggacgtcgac caagccggtg tcgcacctga tcgtcaaggg cggtccgggt
    10981 tcggtgacgg ccacgttcac gccgcccgcc tcgagcggga cgttctccaa cgccggtctg
    11041 cccaaggtcg ggggcggcaa cacccccgac atctccaacg tccagttctg cggtgcgatc
    11101 ccgccggcca cgacgaccac gtcgacgacg accaccgtca agcccacgac gaccaaggcg
    11161 accaccacga cgaaggccac gaccaccact gcggcgccga cgaccaccaa ggccacgacg
    11221 acaacgaagg ccacgaccac caccgccgcg ccgaccacca ccaaggccac gacgacaacg
    11281 acggccacca ccaccaccgc cgcgccgacg accaccaagg ccaccacgac aacgaaggcc
    11341 acgaccacca ccgccgcgcc gaccaccacc aaggccacca ccaccacggc cgctccgacg
    11401 accaccacca cctcgacgac gaccacgtcg acgacgacca cctcgacgac gaccaccacg
    11461 gcagcgccga cgaccaccac atcgacgacg accaccacgg ccgcgccgac gaccaccgtc
    11521 gccggggcca cctcggtccc gtcctcgacg tcgagttcga cgagcagcac gtcgacgagc
    11581 acgacctcga cgagcacgac catctcgccg tcggaggtcc gcagcgagac gtcgatcagc
    11641 aaggacgaca acgtgcgggt cctcgccgac ggcaccgtcc agccgctcgc cgtgaccggc
    11701 aacacctcgc tgccgctcgt gatcgtgggc atcgtgctcc tcgcagcggg cgcggcgatg
    11761 accgtcgtga cccggctccg gacccgccgc accgacgcct gaggccgggt ccgaccccac
    11821 cgacggcgag ccgctggtga cacctgatcg cggccgaggc ctccctcgct ggtgcggggc
    11881 ctcggccgtg gtgctctcgc tcgcggtcgc tgcggcgatc gtcgcgacga gcgacgagca
    11941 tcgactcctt gccgtcctgc tcgcagcggc gacggtgtgg tcggcggcgc cgctcgtcgt
    12001 ccttctcctg tcggggaccg gaacagtggg ggcaaccgac ccgacgccgg cgggaccgac
    12061 gccgaccggg ccgccatcgg cggcgttcac gacaatcgtg cgcgtcggcg acgaaccgct
    12121 cgacatcgcc cgcactacga tcctgctcgc tgcagccgcc gggcccacca tcgtcctcac
    12181 cggaccgggt gcgccgccgc agctgcaccg cctcggagtg ccggtcatcg aggacgccga
    12241 tgccgctgca gggctcgtcc gggccgtggc cagggtcgac acctcggcaa tcctcctggt
    12301 gtcgggccgg gccgttcccg tcgccgacgc gtgccgccgt ggcgccgccc gcctcgagcc
    12361 tggcatcggc tgggtaaccg gtcgaaccgc gccgttcagc cgcgaccgct acgtcggcga
    12421 cagccgccac gacgtcaccg tcgcactcgt cgcggccgca cggcgacgag gcctcgtcgc
    12481 ctgggagccc gacgcgacga tcatcgccac cgaactgctc cgctcccacc ccctcgacgc
    12541 tgaccgtccc ctcggcagct ggctacgacg tcgcgccggc gagggctggg gcggagcggc
    12601 gctcgacgaa ccgatcgccc gccgggcggc gcccgtcgat gctgcggcct actggcccga
    12661 cacgatcgcc cgtcagtggg gccgcacggc cgatctcgcc gcagcgatcg gcactggatc
    12721 gacgcggtgt cgactcctcg ccgccggact cacgctccgc gaactgttcg ccgtgccgct
    12781 gctcttcgcg tcggccctcc ccctgttcgc cgccggtccg accaccccgt cgacgacgac
    12841 ggtcatggtc gcctccgtcg ccgtcgccgc ggcggggatc cggtgggccg cgctccgaca
    12901 gggactcggg ctcccgatgc gccccctgcg cgacgtcacc gccgcgatcc acagcacacc
    12961 cggttctctc gtcgctgtcg gcgctgccgt gacccgaagt cgccgcgccc cgcgcctggc
    13021 gctccccgag cggccgctcg tctgggcggc gctcctcctc accgtcgtga ccggcgcccg
    13081 gctcgtcgac gggacgaccg cgcggccgtc cgccccggtc atggggacgg cgcttgcggc
    13141 gctcgccgca ctctgggcgt tcagcatggg agccctggcg caacgcaact gggcgcggag
    13201 cacctaccgg gtgccgctcg acgtcgggtt ggtgtccgcc gggacgacgt tccggaccgt
    13261 caacgggtca ccgtcagggc tcgcgctcgt ccggctgtgc gacggacccg acgtcgacgc
    13321 gacggccagt cggttggccg tgtcgatcgc gctcgacgac ggcaccgcga tcgacgcgtc
    13381 ggcacgagtc tcgggccgtc gccgctccgg cgaccgcgag gtcatcggtc tcgtgctcaa
    13441 cctcggcgac cgggcacgtg cagcctgggt cgaccagctc gatcgggcag cccagggctc
    13501 cggtcccgaa cgagccggtg cccgggtcga cgcgctgaca cgaagcccag cggcccccga
    13561 tgacggtcct cgacgcggtc gggccgcacg ggtactcgac ggggccggcg ccgcgcttgc
    13621 agcgatcgtc tccctggtgg tggtcgccgc actggtcctc gtgctgttcg gcttccatcc
    13681 gctcgtcatc cggagcgcgt cgatgacacc gacgtacgcg atcggcgacg tcgtcgtctc
    13741 cgagaacgtt ctggccggcg acctgcgccc cggggacgtg gccaccgtcc ccgccgacgc
    13801 gatcggcgaa accctgaccc atcgcgtcgt ctcggtcgct ggagtcggat cgtcggtcac
    13861 cgtcgagacg cgtggcgatg ccaacacgac gggcgagcgc gtggttctcg atgccggcac
    13921 gaccgtcggg cgcgtgcgtt ggtcggtgcc ctggctcggg cgccccgcca actccttgcg
    13981 aagctcgccg gtgcgggtgg cgctggtcgg ggcggccgct gccctcctga tcaccgccgt
    14041 tcgccgccgg gtgcgctgac cggtcgaggc gctgtcctca ggactgcgga actacgctcg
    14101 ccgggtgttc ccgacccgct tcgacagcat cctcgatctc gcccgccttc cctggttcga
    14161 ggtgcagggc ggcaaccgcc tcgtcgccga cccccaggtc gggcccgtca tcgacatgca
    14221 cacccatctg gcgatggcgt acctggctcg gcatcgggtc gacgtgctcg ccgagacggc
    14281 acgcaccgcc tactacctgc cgttgacgat gccgttcgac ctgtcgccgt actcgaacct
    14341 gaacttcgac gccgagtcga tgttcgccat gaaggccgat ctctccgtgc tctccctcac
    14401 cccgtggggc aagcgcgtca cgcacaccgc acccaacctg ctgcgcgaca tggacgacct
    14461 gggtatccgt gatgcggtcc tcctgccgtt cgacctcccc gtcggcccat cgaacgccga
    14521 cgtgttcctc gccgcggccc gccggcatcc tcgactccat tcgttcgccg gcgtccatcc
    14581 gtcggaccgc cgactcgagc agcgcctcga cgagcagatc gccgccggtg ccgccggcct
    14641 caagtttcat ccgaacggtc agttcatggc tgccgaccac cccaagaccg tgcacctctg
    14701 ccgtcgctgc ggcgaccgag gccttcccgt gctcatgcat tgcggaccgg tcggcatcga
    14761 accgaaagct gccgagcgtc gcagccaggt cgcccgatac gagcaggcga ttgccgagaa
    14821 ccccgacacc acgttcattc tcgggcactg cggggccctc cagcacgagg tggcgctcga
    14881 ctcgttcgtc ggtcgctacg acaacctcta ctgggagctg tcgagcctcg gcctcgacgc
    14941 gatgcggacc gtgttcgatc gggtcgctcc cgatcgtctg ctctacggca ccgactggcc
    15001 cttctaccac caggccatca cgatggcccg ggtcctgatc ctcgccgagg acaacgacgc
    15061 gctccgccga gcagtcctgc acgacaacgc agcgcgactg ctgggcctcg acagcgcctg
    15121 acgctggtcg gccggccgtc tgccggcgct cgccggccgg cgctcgaccg gcggcgctca
    15181 accggcagcg gagttgccgg ccggcagttg ggcggccgca atccggcgga tcgaaccgtc
    15241 ggggttggtg tacttcagga cgtacgagcc ccgctggctg agccggcggt cgatcgtcac
    15301 ggcgatggtc tcgtcggcca caggagccgt cgccttctcg gaacgttccc cgaaccgcac
    15361 cacgacgtcg acggtggcct ccgggtaccg gtcccggaac accgacgcca acgacagttg
    15421 cacggtctcg ccgtccgcgg tgtagcccac gccggcgacg tcgtcctcgg gctggtcggt
    15481 cgtcgcgccg ctcaggacgt agccgtcgtc ggtcaggccg aggtagagct ggagggtcag
    15541 gtcgcccccg accgtggcgc tgaacccgca ccgttcgctg ctgagcgcga acgacgccgg
    15601 atcgacgtcg acgccccact tggcgagcgc catggcgagc tggttcggga cctcggccag
    15661 cgtctcgccg agcagtccgt cgatgccgcc gggccacagg tgagggatcg ggtcctcgct
    15721 gagttcgcac tcggtgtcgg gggtacacgg gtcgcccccg cggccgagcg gatcgtccca
    15781 ggtggcgtgc aatgggtcga acccgacgat cgagccgttc cggaccgtga tcgcagcagg
    15841 accctggccg gtgatggaca cgtcgtcggc gaccggcacg tcggagaact ccagcgccgt
    15901 gccgccctcg acgacggcga cgtcgacggc ggcgaccccg atcacgggcg agatgagcac
    15961 caggtaggtg ccgtcgggcg gttccgccgc gccttcggtg ccctcgacgg cagccggctt
    16021 ctgcacgcac gggcggagcg tcgtggtcgg aggcgtcgcc cgctcggtca cgggaaccgt
    16081 cggctcccgc gtcgccacga acaccccggc ggcgacgagc acaccggcgg tggcaagccc
    16141 gatcaacacc atcggccaac ggcgctgccg agacggccgt cgctccgcgt ccatgggacg
    16201 cgagactact gacggttcgt caccgctcgc cccgcagggt tcgtcgggcc cctggcgcac
    16261 cgcaccccac ggggatcagg tcgagatcaa ctcccgcagg agggatgcga tttcgtcgac
    16321 atcgatcaat ggatcgtggt tgaccacgga atgcaggagc gctgccgacc tggtgatgag
    16381 aatccggacc cgacgatcgg gcgaggccac ggggcccggg aacggcgaaa cccccgccga
    16441 tggcgggggt ttccgacgtc gggctgacag gatttgaacc tgcgacccct tgacccccag
    16501 tcaagtgcgc taccagtctg cgccacagcc cgtgacgggg agccactcta gtcgacggtg
    16561 ccggtccact cggaaacgag ttgggaactt caggctcacc cgtgcgaatg cgcacgggtg
    16621 agcctgaagt tggccgcgct tcgagccgct tggagccgct tcgaccgtgg tgacgcctac
    16681 gaccgcaggt acgcgaggac cgcgagcacc cgtcggtgtt gcccttcgtc gggcgggagc
    16741 ccgagcttcg cgaagatgcg ttgagtgtgc ttctcgacgg caccgtcact cacgaccagc
    16801 gcctgagcga tcgaggcgtt ggaccggccc tcggccatga ggccgagcac ctcggtctcc
    16861 cgtggcgtca gggtcgtgac cgggtcgtcg ccgcgacgcc ggaccatgag ctgggacacc
    16921 acctgcggat cgagcacggt gcccccggcg gcaacccgct cgacgctgtc gaggaactcg
    16981 tcgatggcgg acacgcggtc cttcaacaaa tacccgactc ccccgttgcc ggtgcccaac
    17041 agctcgtccg cgtaggacac ctcgacgtac tgcgacagga ccatcacctt ggcctcgggc
    17101 cactgggagc gcacctgcac cgaggcgcgg agaccttcgt cgctgtgcga gggcggcata
    17161 cggacgtcga ccaacgccac gtcgggtcgt tcggccagga cggcctcgac gaacgacgga
    17221 ccgtcaccga cggcggcgac cacctcgtgg
//
LOCUS       DAOSOA010000023        17159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3352_length_17159_cov_6.489535, whole genome shotgun sequence.
ACCESSION   DAOSOA010000023 DAOSOA010000000
VERSION     DAOSOA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17159
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3352_length_17159_cov_6.489535"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKY13_03015"
     CDS             <1..226
                     /locus_tag="PKY13_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT78926.1"
                     /translation="AVMASLLGRVGATRGSIVTYLMPPVSIVLGVLVRNDHIAWLSVV
                     GMAVVLAGAVLVSRKEVPAALETEIVVEGT"
     gene            complement(242..955)
                     /locus_tag="PKY13_03020"
     CDS             complement(242..955)
                     /locus_tag="PKY13_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78927.1"
                     /translation="MKTLAVYQTLLRGVSSRARVIGFAVVGLVEIGIAILLSNANRFD
                     PTSSAVQIVDKFGLTLIIPLAALVFGTASLGDPIEDGTYVYLWLRPIRRWQLTVAAYL
                     AALTMVLPLAVLPTVISGAIVDPTPNMAVGALAASLLAAVAYCAVFVLLGQVTQRSLI
                     WGVAYLLIFEQFISRGGKGLGFVAVHSHAVSVLSKTVGDVDIQLDYFSRPVAVGVALG
                     FTVAWLVWSSVQQNRMSVA"
     gene            complement(952..1965)
                     /locus_tag="PKY13_03025"
     CDS             complement(952..1965)
                     /locus_tag="PKY13_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78928.1"
                     /translation="MTTTPELTGTPAPAPSPPAFAEFRDESSMVAVRHVSKWFKGLVA
                     VSDVSFDVGPGVTALLGPNGAGKSTLLRMLCGLTPPDLGEVRLLGRDPRHDNQVLKYV
                     GLVPQQEGVFPRMNALDFVRLAGVLHDVPDPDGAARAALASVEMDPDESKPVDTYSKG
                     MRQRVKVAQALVNGPSVIMADEPLNGLDPRQRLRLIEIFQRLGAEGRCVIISSHVLDE
                     VERFGSRVLVVAQGRLAAEGDFRGIRELMDDRPHLIRVRSDRCRALVATLIQSGLVSG
                     ARLLSEDAADVSVDDVARFRRSVAPAARHVGAVLTEVSPLDDDLESVFRYLVGAPGPG
                     GVS"
     gene            complement(1962..2966)
                     /locus_tag="PKY13_03030"
     CDS             complement(1962..2966)
                     /locus_tag="PKY13_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT78929.1"
                     /translation="MTDFPPVAAPTPPQAGSAARILDRGYRRYDGERGGIGSSMRSVI
                     RYTAQRVLGIHRKFRFKVMPILTIVISYVPHMVYVGIVVLTNRLENEQAQMTGPGTIP
                     QGAGRLVANQLIQDYPGSYLNISAAIMLFAAFIAPEVLCTDRRTGMLGLYMASPLNRW
                     NYLSAKALAVGMVLLTVTLGPPLLLLIGYSTQGYGPSSAADWASTIARVLATGVGIAF
                     FHTMLSSAISSITTRRAAASAAFIALLTGTGVLVPLVIEEADAPRWLGMFDLVRLPTE
                     FAYRVFGQTSDIGYSTRQFGYGFQVPTDTWVIYAGFFGWTALAAVVIWDRYRRVQVTK
                     "
     gene            complement(2963..3892)
                     /locus_tag="PKY13_03035"
     CDS             complement(2963..3892)
                     /locus_tag="PKY13_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT78930.1"
                     /translation="MNSPIITGTGIVKRYGTNTVLDHADVEVHSGVTGLLGSNGAGKT
                     TMLGLFLGLHRMDEGTLSVLGHDPRVAGHNVRARIGYSPEHHNLPPDLPATDFVQHIA
                     EVHGMPPTDAVGRASDALWFVGMGEERTRPLGTLSTGQRQRVKLAMAIAHDPELVMLD
                     EPTDGLDPTQRETMLDLIRRLSSEFGMNVLLSSHLLDEVERTCNQVIILKDGRVTAAG
                     GIDELKGAGKGLTVEVDWGATELADRLRSRGLTVAQEASRLVVTADASLGPVSQDDLF
                     RVARDDVSDLDLPLRRLQNRTVSLEEVFLEVGT"
     gene            4011..4412
                     /locus_tag="PKY13_03040"
     CDS             4011..4412
                     /locus_tag="PKY13_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78931.1"
                     /translation="MDLVYDEPDDADEPDESEPANTTAIVAADDPLLEEFDEAYEAEL
                     AEALDERSEILPSSGVQKFRRNTAMGAVLNGMALGLRDVFDPVKREEAPIVQDADGEP
                     DHPRHVDAHLDPDDPSASSVTVRPWLAPSEK"
     gene            4426..5691
                     /locus_tag="PKY13_03045"
     CDS             4426..5691
                     /locus_tag="PKY13_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOT78932.1"
                     /translation="MSTEHTGHDDPPSHEAVNLYIDAAADVIELSGEMRVALREPARE
                     IAVQIPVRLDNGAVRMVRGFRVQHNDSRGPYKGGIRYHPSVDQNEVRALASLMTWKTA
                     LVDVPFGGAKGGIEVDPSELSPYEIERMTRRFTLMIGSILGTYQDIPAPDVNTNAQVM
                     AWIMDEYSDRFGYSPAMVTGKPLALGGAPGREAATGLGCVYVLEAFCEHHHLSLIGKR
                     IAIQGFGNVGRHMAWELYKRGAKVVAVSDVSGGVADQGGLDIPALKAHADTGGLLVDA
                     GHGDRITNAELLAFECDFLAPAAMGEVITAANAADVSARVVLEAANYPVTPSGDAVLE
                     SKGITVIPDILANAGGVTGSYFEWTQNIQQFTWKLERFNAELSDRMKLASVTVHRFAE
                     EHGCTLRQAAYGIGLGRVAEATRIRGYLS"
     assembly_gap    5716..5815
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5816..6331
                     /locus_tag="PKY13_03050"
     CDS             <5816..6331
                     /locus_tag="PKY13_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOT78933.1"
                     /translation="VAGDDIGFAYIKATEGRDVSDRRFASNWTAARAAGLEVGAYHYF
                     TLCSDAAAQADHFLETAPSGPDDLPPVVDLEDLDGACDPSAVEVRSEVQRFVTEVERR
                     TDRRIVLYVGEDFEARFGVKEELTRYLWERRLFRRPDVDGWLIWQAHWRARVNGIDGG
                     VDLDVMRPADG"
     gene            6380..>7119
                     /locus_tag="PKY13_03055"
     CDS             6380..>7119
                     /locus_tag="PKY13_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT78934.1"
                     /translation="MSSESGAAIEFVGVSKSFGDGAPAIGHLDLSIESGELVTFVGPS
                     GCGKTTTLRLINRLIEPSGGTISVGGRDVTSVPVEELRRDIGYVIQQVGLFPHRTIAQ
                     NIATVPKLLGWSKPEITERVEELVDLVGLDRSLLTRHPAALSGGQQQRVGVARALAAR
                     PAVLLMDEPYSAVDPVVRAHLQDELLALHGRVETTIVLVTHDIDEAVRLGDRVAVFAN
                     GGRLAQYATPDELMAAPADEFVESFLGA"
     assembly_gap    7120..7219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7220..7666
                     /locus_tag="PKY13_03060"
     CDS             <7220..7666
                     /locus_tag="PKY13_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT78935.1"
                     /translation="IPVFGIGYTVPAIALTTYSLVVLTPFLVVAFDEVDRATLDAADA
                     MGMTRAQRLFSVELPLALPAIFAGLRVTAVTIIGLVTVGGLFGLGGYGDFIDEGLTRD
                     FPTLIVVGVVLSVALALIVDALLVGLGALISPWQRSARAARRAGVV"
     gene            7666..8325
                     /locus_tag="PKY13_03065"
     CDS             7666..8325
                     /locus_tag="PKY13_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT78936.1"
                     /translation="MGSAFEFIFSSTNWSGQDGIGERLGIHLWVSFTALLAAGVLAVP
                     IGLVLGHLRRGRVFAVAVANLTRAIPSLAVLAFVVAAGWGTGFLPTWIAMIALAVPPL
                     FIGALVGVSEVDDAVIDSARGMGMRGWQILWNAELPLATPLLMSGLRVAASQVIATAT
                     LGAFVGYNTLGRFITVGRANGDDPMLYGGVVVIVATALVIDSALRLASRRSLRWRKSE
                     Q"
     gene            8444..>9337
                     /locus_tag="PKY13_03070"
     CDS             8444..>9337
                     /locus_tag="PKY13_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT78937.1"
                     /translation="MPIPTSSTSRRKRPTLRTALVLGLAVLPLTTLAACGEDDTASGD
                     DKGSITIGHQAFGESEIVAHIYGDVLESDGFTVKYQSFKERSAIYAAFESGDIDLVPE
                     YAASGLEFLNKNAGEASSDIVATAEALDAQLAKIDLAAGAASDAVDTNSLVVTADTAK
                     DKSLTKISDLSADLKLGGPQDCPSNASCIPALKEKYDLDFTANFVPLDLGGPLTKAAL
                     TEGDIDVAVLFSTDAGIAANKWVVLEDDQGIFNADNILPVGTKSFVDGNTATLDKVSK
                     KLTTANVTALNKRYDIDKDDAD"
     assembly_gap    9338..9437
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9438..10541
                     /locus_tag="PKY13_03075"
     CDS             <9438..10541
                     /locus_tag="PKY13_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOT78938.1"
                     /translation="ILGDLVSSGLGVNGFSWATSPAVTELETLMLDWMVDLLDLPDHF
                     RGNGVIQDSASSATLCAILAARQRAGVAADRLVAYCTAQAHSSIEKGLRVAGVGIDQI
                     RVVPHDDAFAMVPDALAAAIATDVADGLTPFFVCANAGSTSSEAFDPVPDLAPVCTAA
                     GAWLHVDGAMCGVAALCPELRWVNEGLDLADSYTTNGHKWMGVGFDCSFFWVRDRAPL
                     LGALSILPEYLRTAQAEAGAVIDYRDWQVPLGRRFRSLKVWFVLRLDGAEQIADMIRR
                     HIELATEFESWVRADERFEVIAPRSMNLVCLAHRDGDDATKALVEAVNASGRAQVTPT
                     VLDGRSALRFCVGSHLTERHHVEAAWELISSLA"
     gene            10636..12051
                     /locus_tag="PKY13_03080"
     CDS             10636..12051
                     /locus_tag="PKY13_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78939.1"
                     /translation="MPESSETGRVRRVLRSRTATFLVVFVVFCVSPNVIPHGDPFLVV
                     PTARSLVHDGDLDLTEYLNVPAVLSHYGLTREGDRFVDYFPWVTAATAVPVTLGVDAI
                     SAVAGTPDTDELIRTDRTWPIQLIGGSLLAAGATLLIALVSEALIDRTTRVAATTGRR
                     PPLLALALAVGLTTPLWSTVSRGLWQHGPSLVFLAGALLVLLRVERSPTLRRIDGRLV
                     GVGALAGAAYWARPTNAVAVVAIGLIALVMGQRSALTYAAGAAGAFTAGVLANLALVG
                     VAVPKYYAAGRIGWHREFVEAFLANLVSPSRGLLFTLPQIVTIGAVLTHPDQRSWSRV
                     TRRLVFVGPLVPLGYALAVSGFANHWWAGHSYGARFLIESLPFVLPIAVVGSLRLLQG
                     RGALAAGTVVLWSLAGVVHFEGAWFHSTTCWNGSPTDIDLDPGRVWSVTDPQFASGFA
                     SLVEDGPADALWRSCENGAGG"
     gene            complement(12048..12989)
                     /locus_tag="PKY13_03085"
     CDS             complement(12048..12989)
                     /locus_tag="PKY13_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOT78940.1"
                     /translation="MSTPPSTVSIVEVAPRDGLQSDPTLLGTDAKVEMITRAVRAGIR
                     RIETVSFVNPKRVPQMADADDVMAALLADTETRALGASYIGLVLNQRGLDRAVETGVD
                     EINAVVVCSDTFAMKNQGQDSQGLLDTAASVVAGARAHGLPVSVTMAASFGCPYEGEV
                     SLNRLAWVVEQVVAAGPDEIALADSIGVAVPTDVAARVALVRDIVGDAPITLRCHFHN
                     TRNTGLANAAAALDAGVRVFDSSLGGIGGCPFAPNATGNVPTEDLLYMFHRMGFDSGV
                     DLDALAANVPWIEDVLGHSVPGYLSKAGNFPGALRAS"
     gene            13015..13398
                     /locus_tag="PKY13_03090"
     CDS             13015..13398
                     /locus_tag="PKY13_03090"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT78941.1"
                     /translation="MTDPSPAFLWEVVVDCAEPATLGRFYADLCECDLHQRDADWAWI
                     EPATRGGLAAGAGGPRLAFQRVPEPKVGKVRLHLDLGATDVSATVARAVEIGATQLAD
                     PVTDESGTYVVLADPEGHEFCIVDA"
     gene            complement(13410..14000)
                     /locus_tag="PKY13_03095"
     CDS             complement(13410..14000)
                     /locus_tag="PKY13_03095"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT78942.1"
                     /translation="MARDAAATQQRIIDAATDEFAAHGISGARVDEIAAKADVNKRML
                     YYYFGSKEQLFREILRRKLAERVAEAAGSVELSRADRLAERQALHLEDSSWVRLLMWE
                     ALEYGADTELEDEVGRGEIYGQWVAATEQAQADGEVAADLDARQLVLTELALTLFPAA
                     FPQLTRLVSGTELDDPEFLEARQTFLRRLSDRLAGP"
     gene            14056..15645
                     /locus_tag="PKY13_03100"
     CDS             14056..15645
                     /locus_tag="PKY13_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOT78943.1"
                     /translation="MAFSITPEQAHEKRWWTLGALCLSLSIIGIDNTILNVALPSIVK
                     SVGAEGSQLQWIVDSYVIVFACLLLTAGTLGDKLGRKRMLLIGLALFGGFSAVAALSD
                     SANTLIIFRALMGIGGAFIYPSTLSILTNTFTGKERAKAIGIWAGVSGLGIAIGPLAG
                     GILVEHFFWGSVFLVNVPICVLAIVMTMAVVPESRDKSDSHLDPLGAVLSILGLSGVL
                     LAIIEAPERGWGDSVVIAGFLLGASFLVGFAVWERRAAHPMLDVSFFKDPRFTAASGT
                     ITLTFFALFGSTFLLTQYFQFVLGYSPLKAGMMTAPVAIGIMLAGTNAPKIAARIGTK
                     YTVVLGLVVLSLVLMSYASTALMSSVLWGSLVRVVFGLGMGTIMAPATESIMSSLPRN
                     KAGVGSAVNDTTRQTGGALGVAVIGSIFAARYHHLISPPAGLTAAAEAAVRDSIGQAR
                     GVAAQPGVPAALADQIREAASSAYVGGMQLAVFVGAGITLFAAVIAWKYLPTRSASPE
                     QQAAEELELAERGQATVEALV"
     gene            15689..16594
                     /locus_tag="PKY13_03105"
     CDS             15689..16594
                     /locus_tag="PKY13_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78944.1"
                     /translation="MKGFHARVVVLAVLGCTALSSGCLPLPPAETAAICNTAPSGPPT
                     ADDLAVLERLADRDLPVAIGSPPSVRLDAHDTATATALRAEFGASIDIEIGSKRLGRV
                     PGNPDGCLLVAPTWAIAEPAPKGVTMTATPTQPVFDRNGSIRATVRVTNRTGRSIVVS
                     QAVPRTPSRNCGDLGVALSSTTSALAVNRVDGLQPLDPNFQLVRTGRSYGRLSRMGDG
                     SFSTVIVTSICAIPIVTGITVRPGGSAMIPVTAGTESVQPGADPIVLPGTYHLRATVD
                     VRLSTAPDAVAHRVAAPPTRVELRD"
     gene            16641..>17159
                     /locus_tag="PKY13_03110"
     CDS             16641..>17159
                     /locus_tag="PKY13_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78945.1"
                     /translation="MIAGIPRPRRRRATLAGSLLTIALFVSSCVPPATLPDGSVPQTG
                     PIGANGVGFDGTDLWLCDLFGGQLLRIDPGSGRILERIGPDRGIARPDDIVFAPDGSL
                     IYTSPPTGVVGRITRSGPNAGQVSVLAQMETGVNPIALTPDGSAVIVGYGSGEVGRLD
                     RIDLATGQVTVVA"
BASE COUNT         2542 a         5974 c         5697 g         2646 t
ORIGIN      
        1 cgcagtgatg gcctcgctcc tgggccgcgt cggcgccacc cgcggctcga tcgtcaccta
       61 cctgatgcca ccggtgtcga tcgtgctcgg cgtcctggtc cgcaacgacc acatcgcctg
      121 gttgtcggtg gtcggcatgg ccgtggtgct ggcgggcgcg gtgctggtca gccgcaagga
      181 ggttcccgcc gcgctcgaga ccgagatcgt ggtcgagggc acctgaccac gccgacgggc
      241 gtcaggcgac gctcatccgg ttctgctgca ccgacgacca gacgagccac gcgaccgtga
      301 agccgagggc aacccccacg gccacgggcc gcgagaagta gtcgagctgg atgtcgacgt
      361 cgccgaccgt cttcgacagg accgacacgg cgtggctgtg cacggcgacg aacccaagtc
      421 ccttgccccc tcgcgagatg aactgctcga agatcagcag ataggcgacg ccccagatga
      481 gcgaccgctg cgtcacctgc cccaacagga cgaagacggc gcagtaggcg acagcggcga
      541 gcagcgaggc cgccagtgcg ccgacggcca tgttcggggt ggggtcgacg atggcgccgc
      601 tgatgacggt cggcagcacg gcgagcggga gcaccatcgt gagcgcggcg aggtacgcgg
      661 cgaccgtcag ctgccaccgc cggatcggcc gcaaccagag atagacgtag gtgccgtcct
      721 cgatcgggtc gcccaaggac gccgtgccga acacgagcgc ggccaacggg atgatcaggg
      781 tgaggccgaa cttgtcgacg atctggacgg cgctcgacgt cgggtcgaag cgattggcgt
      841 tcgagagcag gatcgcgatg ccgatctcga cgaggccgac gacggcgaag ccgatcaccc
      901 gggcccgact cgacacgccg cgcaggaggg tctggtagac ggcgagggtc ttcacgacac
      961 tcctcccggg ccgggcgcgc cgacgaggta ccggaagaca ctctcgaggt cgtcgtcgag
     1021 cggggagacc tcggtgagca ccgcacccac gtggcgcgcc gccggcgcga ccgagcggcg
     1081 gaatcgggcg acgtcgtcga cggagacatc cgcggcgtcc tcggacagga ggcgcgcccc
     1141 cgacacgagc ccactctgga tgagggtcgc gacgagcgca cggcaacgat cgctgcggac
     1201 ccggatcagg tggggacgat cgtccatgag ctcgcgaatg ccccggaagt cgccctcggc
     1261 cgcgagccgg ccctgggcga cgacgagcac ccgcgacccg aaccgctcca cctcgtcgag
     1321 gacgtgcgag ctgatgatga cgcagcgccc ctcggcgccg agccgctgga agatctcgat
     1381 gagccggagc cgctgacgag ggtcgaggcc gttgaggggt tcgtcggcca tgatcaccga
     1441 cggtccgttg acgagtgcct gcgccacctt cacgcgttgg cgcatgccct tggaataggt
     1501 gtccaccggc ttcgactcgt cggggtccat ctcgaccgag gccagcgcgg cccgagccgc
     1561 accgtcggga tcgggcacgt cgtggagcac cccggcgagc cgcacgaagt cgagcgcatt
     1621 catccgcggg aacacgccct cctgctgggg gacgaggccg acgtacttga ggacctggtt
     1681 gtcgtggcgg ggatcacgcc cgaggaggcg cacctcgccg aggtcgggcg gcgtgagacc
     1741 acacagcatg cggagcaggg tcgacttgcc ggcgccgttg gggcccagca gggcggtgac
     1801 gccggggccg acgtcgaacg acacgtccga caccgcgacc agccccttga accacttgct
     1861 gacgtggcgc accgcaacca tcgacgactc gtcgcggaac tccgcgaacg ccggcggtga
     1921 cggtgccggt gccggcgttc ccgtgagctc gggcgtggtg gtcacttggt cacctgcaca
     1981 cggcgatatc ggtcccagat gacgacggcc gccaacgccg tccacccgaa gaacccggcg
     2041 tagatcaccc aggtgtcggt cggcacctgg aacccgtacc cgaactgccg ggtcgaatag
     2101 ccgatgtcgg aggtctggcc gaacacgcgg tacgcgaact cggtcggcag tcgcacgagg
     2161 tcgaacatgc cgagccaccg gggagcatcc gcctcctcga tcaccagcgg cacgagcact
     2221 ccggtgccgg tcaggagggc gatgaacgcc gccgacgctg cagctctccg ggtcgtgatc
     2281 gaactgatgg cgctcgagag catggtgtgg aagaacgcga tgcccacacc cgtcgccaac
     2341 acgcgggcga tggtcgacgc ccagtcggcg gccgacgacg ggccgtatcc ctgggtggag
     2401 tagccgatga gcaacaggag cggcgggccg agggtgacgg tcagcaacac catgccgacg
     2461 gccagggcct tggccgacag gtagttccag cggttgagcg gcgacgccat gtagaggccg
     2521 agcatgcccg tccgccggtc ggtgcacagg acctcgggcg cgatgaacgc ggcgaagagc
     2581 atgatggcag cgctgatgtt gaggtagctg ccgggatagt cctggatgag ctggttggcc
     2641 accagtcggc cggcgccctg agggatcgtg cccggccccg tcatctgcgc ctgctcgttc
     2701 tcgaggcggt tcgtgaggac gacgatgccg acgtacacca tgtggggcac gtaggagatg
     2761 acgatcgtga ggatcggcat caccttgaag cggaacttgc ggtggatgcc gagcacgcgt
     2821 tgggcggtgt agcggatgac gctgcgcatc gaggaaccga tgccaccgcg ctcgccgtcg
     2881 tagcggcggt acccgcggtc caagatgcgc gccgcacttc ccgcctgcgg cggggtcggc
     2941 gcggcgacgg gagggaaatc ggtcatgtgc cgacctccag gaacacctct tcgagcgaca
     3001 ccgtgcggtt ctgcaggcgg cggaggggaa ggtcgaggtc gctgacgtcg tcacgggcga
     3061 cgcggaacag gtcgtcctgg ctcacggggc cgagcgacgc gtcggcggtg acgacgagac
     3121 ggctcgcctc ctgggcgacg gtgagcccgc gcgaccgcaa tcggtcggcc agctccgtcg
     3181 cgccccagtc gacctcgacg gtgaggccct tcccggcacc cttcagctcg tcgataccgc
     3241 cggccgctgt cacccggcca tccttgagga tgatgacctg gttgcaggtg cgctcgacct
     3301 cgtcgaggag gtgcgagctg agcagcacgt tcatgccgaa ctccgacgag aggcgtcgga
     3361 tcaggtcgag catggtctcc cgctgggtcg ggtcgaggcc gtcggtcggt tcgtcgagca
     3421 tcaccagctc ggggtcgtgg gcaatcgcca tggccaactt cacccgttgg cgttgaccgg
     3481 tcgacagcgt gccgagcggg cgggtccgct cctcgcccat gccgacgaac cacaacgcgt
     3541 ccgaggcgcg accgacggca tcggtcggtg gcatgccgtg cacttccgcg atgtgctgca
     3601 cgaagtccgt ggcgggcagg tcgggtggaa ggttgtggtg ctcgggtgag tacccgatgc
     3661 gcgctcgcac gttgtgcccg gcgacgcggg gatcgtggcc gagcaccgag agcgtgccct
     3721 cgtccatgcg gtgcaggccg aggaacaggc cgagcatcgt cgtcttgccg gccccgttgg
     3781 aaccgagcag ccccgtcacc cccgagtgga cctcgacgtc ggcgtggtcg agcacggtgt
     3841 tcgtgccgta ccgcttcacg atgccggtac cggtgatgat cggggaattc atcgccgccg
     3901 agcattgcat accgccccca cgctccgaag gcagccgggc accccgagtt gtgagaattc
     3961 ggcgcggagg tggcggcgca atgggcgacg ccggcaggta cggtggcccc atggacctcg
     4021 tctacgacga acccgacgac gccgacgaac ccgacgagtc ggagccggcg aacaccacgg
     4081 ccatcgtcgc ggccgacgat ccgctgctcg aggagttcga cgaggcgtac gaggccgagc
     4141 tcgccgaggc cctcgacgag cggagcgaga tcctcccgtc gtcgggcgtg cagaagttcc
     4201 gtcgcaacac cgccatgggt gccgtcctga acggcatggc cctcggcctg cgcgacgtgt
     4261 tcgacccggt caaacgcgag gaggcaccga tcgtgcagga cgccgacggc gaacccgacc
     4321 atccgcgtca cgtcgacgcc catctcgatc ccgacgaccc gtcggcgtcg tcggtgaccg
     4381 tccggccctg gctcgcgccg tccgagaagt gacgcggcgt acgacgtgag caccgagcac
     4441 accggtcacg acgacccccc gtcccacgag gcggtcaacc tctacatcga cgccgccgcg
     4501 gacgtcatcg aactgtccgg cgagatgcgc gtcgcgctcc gcgaaccggc tcgcgagatc
     4561 gcggtccaga tccccgttcg cctcgacaac ggcgcggtgc gcatggtccg cggcttccgt
     4621 gtgcagcaca acgacagccg tggcccctac aagggcggca tccgctacca cccgagcgtc
     4681 gaccagaacg aggtgcgcgc tctcgcatcg ctgatgacct ggaagacggc gctggtcgac
     4741 gtcccgttcg gtggggccaa gggtggcatc gaggtggacc cgtcggagtt gagcccctac
     4801 gagatcgagc gcatgacccg gcgcttcacg ctgatgatcg gttcgatcct cgggacctac
     4861 caggacatcc ccgctcccga cgtcaacacc aacgcccagg tcatggcgtg gatcatggac
     4921 gagtactccg accgcttcgg gtacagcccg gcaatggtca cgggcaagcc cctcgccctc
     4981 ggcggtgccc caggccgcga ggccgccacc ggcctcggat gcgtctacgt gctcgaggcg
     5041 ttctgcgagc accaccacct gtcgctcatc ggcaagcgca tcgccatcca ggggttcggc
     5101 aacgtggggc gacacatggc gtgggagctg tacaagcggg gagccaaggt cgtggcggtg
     5161 tcggacgtgt cgggcggcgt cgccgaccag ggcggcctcg acatcccggc gctcaaggcg
     5221 cacgcggaca ccggtgggct cctcgtcgat gccggtcacg gcgatcgcat caccaacgcc
     5281 gagctgctcg cgttcgagtg cgacttcctc gctccggcgg cgatgggcga ggtcatcacc
     5341 gccgccaacg ctgccgacgt gtcggcccgc gtggtgctcg aagcggccaa ctaccccgtc
     5401 accccgagcg gcgacgcggt gctcgagtcg aagggcatca ccgtcatccc cgacatcttg
     5461 gccaacgccg gtggcgtgac gggctcgtac ttcgagtgga cccagaacat ccagcagttc
     5521 acgtggaaac tcgagcggtt caacgccgag ctctcggacc ggatgaagct cgcgtcggtg
     5581 accgtccacc gcttcgccga ggagcacggg tgcacccttc ggcaggccgc ctacggcatc
     5641 gggctcggtc gcgtcgcgga ggcgacccgc attcgcggct acctgtcata gcggggcacc
     5701 acgctctgac ggccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtcgc
     5821 cggcgacgac atcggcttcg cgtacatcaa ggccaccgag ggacgcgacg tcagcgaccg
     5881 ccggttcgct tcgaactgga cggccgctcg ggcggccggt ctggaggtcg gcgcctacca
     5941 ctacttcacg ctgtgctccg acgcagcagc gcaggccgac cacttcctgg agacggcccc
     6001 gtccggtccc gatgacctcc cgcccgtcgt cgacctggag gacctcgacg gagcgtgcga
     6061 cccgtccgcc gtcgaggttc gctccgaggt gcaacggttc gtcaccgagg tcgaacgtcg
     6121 caccgatcgg cgcatcgtgc tctacgtcgg cgaggacttc gaggcgaggt tcggtgtgaa
     6181 ggaggagctc acgcggtacc tctgggaacg ccgcctgttc cgtcggcccg acgtcgacgg
     6241 atggctcatc tggcaggccc actggcgggc acgggtgaac ggcatcgatg gaggagtcga
     6301 cctcgatgtg atgcgaccgg ccgacggctg aggtaacacg gcagttccga tcgagaaggt
     6361 cgcggctagg gtccgaccga tgtcgagcga gtcgggcgca gccatcgagt tcgtcggggt
     6421 gagcaagtcg ttcggtgacg gagcgccggc gatcggtcac ctcgacctgt cgatcgagag
     6481 cggcgagctg gtcaccttcg tcggcccgtc gggctgcggc aagaccacca ccctccgact
     6541 gatcaaccgg ctcatcgaac cgagcggcgg cacgatcagc gtcggcggac gcgacgtgac
     6601 gtcggtgccc gtcgaggagc tgcgccgtga catcggctac gtgatccagc aggttgggct
     6661 gtttccgcac cgcaccatcg cgcagaacat cgcgacggtc ccgaagctcc tgggctggtc
     6721 gaagcccgag atcaccgaac gggtcgagga gctcgtcgac ctcgtcggcc tcgaccggtc
     6781 gttgctcacc cgccaccccg ccgcgctcag cggcggtcaa cagcagcgcg tcggcgtggc
     6841 gagagcactc gccgcccgtc ccgcggtgct gctcatggac gagccctaca gcgccgtcga
     6901 cccggtggtc cgggcccacc tccaggacga actgctcgcg ctgcacggcc gggtcgagac
     6961 cacgatcgtc ctcgtgaccc acgacatcga cgaagccgtg cgtctcggcg accgcgtcgc
     7021 cgtgttcgcg aacggtggtc ggctggcgca gtacgccacg cccgacgagc tgatggcagc
     7081 ccccgccgac gagttcgtcg agtcgttcct cggcgccgan nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnna tcccggtgtt cgggatcggg tacaccgtgc ccgccatcgc
     7261 gctcacgacc tacagcctcg tagtgctcac accgttcctc gtggtcgcgt tcgacgaggt
     7321 cgaccgggcg acgctcgacg ccgccgatgc gatgggaatg acccgggcgc agcggttgtt
     7381 ctcggtcgaa ctgccgctgg cgctgccggc gatcttcgcc ggcctccgcg tcaccgccgt
     7441 cacgatcatc ggtctggtca ccgtcggtgg cctgttcggc ctcgggggct acggggactt
     7501 catcgacgag ggtctgacgc gggacttccc gacgctcatc gtcgtcggag tcgtcctgtc
     7561 ggtcgcgctg gcgctgatcg tcgacgcgct gctcgtcggc ctcggggcac tgatctcccc
     7621 gtggcaacga tcggctcgcg ccgcccgccg ggccggggtg gtgtgatggg ttctgccttc
     7681 gagttcatct tcagttcgac caactggtcg ggacaggacg ggatcggcga acggctcggc
     7741 atccacctgt gggtgtcgtt cacggcgctg ctcgccgccg gcgttctcgc cgtcccgatc
     7801 gggctggtgc tcggacatct gcgacgcggc cgcgtgttcg ccgtggccgt tgccaacctg
     7861 acccgcgcca tcccgtcact cgccgtgctg gcgttcgtgg tggccgctgg ctggggcacc
     7921 ggcttcctcc cgacgtggat cgcgatgatt gcgctcgccg ttcccccgct cttcatcggc
     7981 gcgctggtcg gggtctccga ggtcgacgat gccgtgatcg actctgcccg tggcatgggg
     8041 atgcgcgggt ggcagatcct ctggaacgcc gagctgccgc tcgcgacgcc actcttgatg
     8101 tccggtctcc gggtcgcggc cagccaggtc atcgcgacgg ccaccctcgg ggcgttcgtc
     8161 gggtacaaca ccctcggccg gttcatcacc gtcggtcggg ccaacggtga cgaccccatg
     8221 ctctacggcg gtgtggtggt gatcgtcgcc accgcgctgg tcatcgactc cgcgctgcgg
     8281 ctggcgtcga gaaggtcgtt gcggtggcgg aaatccgagc agtagcgtcc atttctcacg
     8341 atgtgaaggg ccggttccac atcgtgaact ggctggggga atgacgacgg actcctcgcc
     8401 gttgacagga ctcgaacccg aaccggcgtc cccaaggagc cccatgccga tcccgacctc
     8461 ttccacctcg cggcgcaagc gcccgacctt gcgcactgcg ctggtgctcg gcctcgccgt
     8521 gctccccctc accaccctcg ccgcctgcgg tgaggatgac accgcctcgg gcgacgacaa
     8581 gggctcgatc acgatcggcc accaggcctt cggcgagtcc gagatcgtcg cccacatcta
     8641 cggcgacgta ctcgagagcg acggcttcac cgtgaagtac cagtcgttca aggaacgctc
     8701 ggccatctac gccgccttcg aatccggcga catcgacctg gttcccgagt acgcggcgtc
     8761 agggctcgag ttcctcaaca agaacgccgg cgaggcgagc tccgacatcg tcgccaccgc
     8821 cgaggcactc gacgcccagc tcgcgaagat cgacctcgcc gccggcgcgg cctccgatgc
     8881 cgtcgacacc aactcgctcg tcgtcaccgc cgacactgcg aaggacaaga gcctgacgaa
     8941 gatctccgat ctctccgccg acctcaagct cggtggtccc caggactgcc cgtccaacgc
     9001 cagctgcatc ccggccctca aggagaagta cgacctcgac ttcaccgcca acttcgtccc
     9061 gctcgacctc ggcggcccgc tcaccaaggc ggccctgacc gaaggtgaca tcgacgtcgc
     9121 cgtgctcttc tccaccgacg ccggcatcgc cgccaacaag tgggtcgtgc tcgaggacga
     9181 ccagggcatc ttcaacgccg acaacatcct gcccgtcggc acgaagagct tcgtcgacgg
     9241 caacaccgcg accctcgaca aggtgtcgaa gaagctcacg acggccaacg tcacggcgct
     9301 caacaagcgc tacgacatcg acaaggacga cgctgacnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnatc ctcggcgacc tcgtcagctc gggcctcggc gtcaacggct
     9481 tcagctgggc gacgagcccg gcggtgaccg aactcgaaac actgatgctc gactggatgg
     9541 tcgacctcct cgacctgccc gaccacttcc gcggcaacgg agtgatccag gacagcgcat
     9601 ccagcgcgac cctctgcgcc atcctggccg cccgccagcg cgccggtgtc gccgccgacc
     9661 ggctcgtcgc ctactgcacg gcgcaggctc actcgtcgat cgagaagggc cttcgcgttg
     9721 ccggggtcgg catcgaccag atccgcgtgg tgccccacga cgacgcgttc gcgatggtcc
     9781 ccgacgcact ggcggccgcg atcgccaccg acgtcgccga cgggctcacg ccgttcttcg
     9841 tctgcgccaa cgccggatcg acctcctccg aggcgttcga tccggttccc gatctcgcgc
     9901 cggtctgcac cgccgccggc gcctggctgc acgtcgatgg tgccatgtgt ggagtcgcgg
     9961 cgctgtgccc tgagctgcgc tgggtcaacg aagggctcga ccttgcggac agctacacga
    10021 ccaacggcca caagtggatg ggggtcggct tcgactgctc gttcttctgg gtgcgcgacc
    10081 gcgctcccct gctcggggcg ctctcgatcc tccccgagta cctccgcacg gcgcaggcag
    10141 aggccggcgc ggtcatcgac taccgcgact ggcaggtgcc gctcggtcgg cggttccgct
    10201 cgctcaaggt gtggttcgtc cttcgcctcg acggagccga gcagatcgcc gacatgatcc
    10261 gccgccacat cgaactggcc accgagttcg agtcgtgggt gcgcgccgac gagcgcttcg
    10321 aggtgatcgc tccccggtcg atgaacctcg tctgcctcgc ccaccgcgac ggcgacgacg
    10381 ccacgaaggc gctcgtggag gcggtcaacg cgagtggacg agcgcaggtc accccgaccg
    10441 tgctcgacgg acggtcggcg ctgcggttct gcgtcggctc ccacctcacc gaacgccacc
    10501 acgtcgaagc ggcgtgggag ttgatcagct cactggcctg aggcccgccg ggaagttgcc
    10561 gccttcgaca ggtagccggg aaccggatcg tgcaggtccc cgggccgacg gggcgatgac
    10621 cgttatgttg atgcggtgcc cgagtcgtcg gagaccggtc gtgtccggcg cgtcctccga
    10681 agccggaccg cgacgttcct ggtggtgttc gtggtcttct gcgtctcgcc caacgtcatt
    10741 ccgcacggcg acccgttcct cgtcgttccc acggcgagga gcctcgtcca cgacggcgat
    10801 ctcgacctga cggagtatct gaacgtgccc gcagtgctgt cccactacgg cctcacccga
    10861 gagggcgacc ggttcgtcga ctacttccca tgggtcacgg ccgccacagc ggtccccgtc
    10921 acgctcggcg tcgacgccat ctcggccgtg gccggcaccc ccgacaccga cgaactcatc
    10981 cgcaccgacc ggacctggcc gatccagctg atcggcggca gcctgctcgc cgccggggcg
    11041 acgctcctga tcgccctcgt cagcgaggcc cttatcgacc gtacgacccg cgtcgccgca
    11101 accaccgggc gacgtccgcc gctcctcgcc ctggcgctcg ccgtcgggct cacgacaccg
    11161 ctctggtcga ccgtctcccg gggcctctgg cagcacgggc cgtcgctcgt gttcctcgcc
    11221 ggcgcgctcc tcgtcctcct ccgtgtggag cggagcccaa ccctccgtcg catcgacggc
    11281 cgactcgtcg gcgtcggagc cctggccggt gccgcctact gggcgcgccc caccaatgcg
    11341 gtcgccgtcg tggcgattgg cctgatcgcg ctcgtgatgg ggcaacggtc ggccctcacc
    11401 tacgccgccg gcgcggcagg agcgttcact gccggcgtcc tggccaacct cgccctcgtc
    11461 ggtgtggccg taccgaagta ctacgcggcc ggacggatcg gctggcaccg cgagttcgtc
    11521 gaggcgttcc tggcgaacct cgtcagcccg tcgcggggac tgctgttcac cctgccccag
    11581 atcgtcacga tcggagcggt cctgacccac ccggatcagc gctcgtggag ccgggtcacc
    11641 cggcgtcttg ttttcgtcgg cccgctcgtt cccctcggat acgcactggc ggtgtcgggg
    11701 ttcgccaacc attggtgggc cggccactcc tacggtgccc ggttcctcat cgagtcgctg
    11761 cccttcgtac tcccgatcgc cgtggtcggg tcactccgcc tgctgcaggg gcgcggcgcg
    11821 ctcgctgccg gaacggtcgt gttatggagc ctcgccggcg tggtccactt cgagggggcc
    11881 tggttccact cgaccacctg ctggaacggg tcccccaccg acatcgacct cgaccccggc
    11941 cgggtgtgga gcgtgaccga cccgcagttc gccagcgggt tcgcctcgct cgtcgaggac
    12001 ggaccggcgg acgcgctgtg gcggtcgtgc gagaacgggg ccggcggcta ggacgcccgg
    12061 agggccccgg ggaagttgcc ggccttcgac aggtagccgg gaacggagtg gccgagcacg
    12121 tcctcgatcc acggcacgtt ggcggcgagc gcgtcgagat cgacgccgct gtcgaagccc
    12181 atgcggtgga acatgtacag cagatcctcg gtggggacgt tgcccgtcgc gttcggggcg
    12241 aacggacagc cgccgatgcc accgagcgag ctgtcgaaca cgcgcacgcc ggcgtcgagc
    12301 gcggccgcgg cgttggcgag gccggtgttg cgggtgttgt ggaagtggca acgcagtgtg
    12361 atgggagcgt cgccgacgat gtcgcggacg agcgcgaccc gggcggcgac atcggtcggc
    12421 actgcgacgc cgatcgagtc ggcgagggcg atctcgtcgg gccccgcggc gacgacctgc
    12481 tcgacgaccc acgccagccg gttgagcgac acctcgccct cgtaggggca gccgaacgag
    12541 gctgccatcg tgaccgacac cggcagaccg tgcgcccgag cgccggcgac cacggacgcc
    12601 gctgtgtcga gcagaccctg cgaatcctgg ccctggttct tcatggcgaa cgtgtcactg
    12661 cacacgacga cggcgttgat ctcgtcgacc ccggtctcga cggcgcggtc gaggccgcgc
    12721 tggttgagca cgagcccgat gtagctggca ccgagcgccc tggtctcggt gtcggcgagc
    12781 agcgccgcca tcacgtcgtc ggcgtcggcc atctgcggca cacgcttggg gttgacgaag
    12841 ctcaccgtct cgatccggcg aatgccggcg cgcacggccc gcgtgatcat ctcgaccttg
    12901 gcgtcggtac cgagcagggt cggatcggat tgcaggccgt cgcggggagc gacctcgacg
    12961 atgctcacgg tcgatggggg tgtgctcacg cggccagttt gccagactcg tccgatgacc
    13021 gatccgtcgc cggcattcct ctgggaggtg gtcgtcgact gcgcggagcc cgccaccctg
    13081 ggccgcttct acgccgacct gtgcgagtgc gacctccacc agcgcgacgc cgactgggcg
    13141 tggatcgaac ccgccacgcg gggcggactc gccgccggtg cgggcggacc acgcctggcg
    13201 ttccaacgcg tgcccgagcc caaggtgggc aaggtccgcc ttcacctcga tctcggtgcc
    13261 accgacgtct cggccaccgt cgctcgggcc gtcgagatcg gggcgaccca gctcgccgac
    13321 ccggtcaccg acgaatcagg tacctacgtc gtactggccg atcccgaagg ccacgagttc
    13381 tgcatcgtcg acgcatgagc gaggccgggt cacggcccgg cgagtcggtc cgacaatcga
    13441 cggaggaacg tctggcgggc ctccaggaac tcgggatcgt cgagctcggt gccgctgacg
    13501 agacgcgtca gctgcgggaa cgccgcgggg aacaacgtga gggcgagctc ggtcagcacg
    13561 agttgccggg cgtcgaggtc ggcggcgacc tcaccatcgg cctgggcctg ttcggtggcg
    13621 gcgacccatt gaccgtagat ctcgccgcgg ccgacctcgt cctcgagttc ggtgtcggcg
    13681 ccgtactcca gcgcctccca catcaggagc cgcacccatg aggagtcctc gaggtggagc
    13741 gcctgccgct cggcaagccg gtcggcacgg ctgagttcga cacttcccgc cgcctcggcg
    13801 acccgctcgg cgagcttgcg gcggaggatc tcccggaaca gctgctcctt cgaaccgaag
    13861 tagtagtaga gcatccgctt gttgacgtcg gccttggcgg cgatctcgtc gacgcgagca
    13921 cccgagatgc cgtgggcggc gaactcgtcg gtcgccgcgt cgatgatgcg ctgctgggtc
    13981 gcagcggcgt cacgagccat aggtcacacc ctaccggcgt tgcccgttaa ctcaccagtt
    14041 agttaccatc gccggatggc cttctccatc actccagagc aggcgcacga gaagcgctgg
    14101 tggacactcg gcgcactctg cctctccctg tcgatcatcg gaatcgacaa cacgatcctc
    14161 aacgtggccc tcccctcgat cgtgaaatca gtcggcgccg agggcagcca gctgcagtgg
    14221 atcgtcgact cctacgtgat cgtcttcgcc tgcctccttc tcacggccgg caccctcggc
    14281 gacaagctcg gccgcaagcg catgctgctc atcggcctcg cgctgttcgg cggcttctcc
    14341 gccgtggcgg cgctgtccga ctccgccaac acgctcatca tcttccgggc gctcatgggc
    14401 atcggcggtg cgttcatcta cccgtcgacg ctgtcgatcc tcaccaacac cttcaccggc
    14461 aaggaacgcg ccaaggccat cgggatctgg gccggcgtgt cgggcctcgg catcgcgatc
    14521 ggcccgttgg ccggcggcat cctcgtcgag cacttcttct ggggttcggt gttcctcgtc
    14581 aacgtgccga tctgcgtgct cgccatcgtc atgacgatgg ccgtcgttcc cgagtcgcgc
    14641 gacaagagcg acagccacct cgacccgctg ggcgccgtgc tctcgatcct cggactcagt
    14701 ggtgtgctcc ttgcgatcat cgaagcgccc gaacggggct ggggcgactc ggtcgtcatc
    14761 gccggtttcc tgctcggcgc atcgttcctc gtcgggttcg ccgtgtggga acgtcgggcg
    14821 gcgcacccca tgctcgacgt ctccttcttc aaggaccccc ggttcaccgc tgcatccggc
    14881 acgatcaccc tcacgttctt cgcgctgttc ggctcgacgt tcctcctcac ccagtacttc
    14941 cagttcgtgc tcggctactc accgctcaag gccggcatga tgacggcgcc tgtcgccatc
    15001 ggcatcatgc tcgccggcac caacgccccc aagatcgcgg cgcggatcgg caccaagtac
    15061 acggtcgtgc tggggttggt cgtgctgagc ctcgtgctga tgagctacgc ctcgaccgcc
    15121 ctcatgagct cggtgctctg gggctcgctc gtccgggtcg tgttcggtct cggcatgggc
    15181 acgatcatgg cgccggccac cgagtcgatc atgagctcac tcccccgcaa caaagccggg
    15241 gtcgggtccg ctgtcaacga cacgacccgc cagacgggcg gtgcgctcgg cgtggcggtg
    15301 atcggttcga tcttcgccgc ccgctaccac cacctgatct ccccgccggc cggactcacg
    15361 gccgcagccg aggccgcagt gcgcgactcg atcggtcagg cccggggcgt ggccgcgcaa
    15421 cccggcgtgc cggcggccct ggccgaccag atccgcgagg ccgcatcctc ggcatacgtc
    15481 ggcggcatgc agctcgccgt cttcgtgggc gccgggatca cgctgttcgc tgcggtgatc
    15541 gcctggaagt acctgccgac ccgctccgca agccccgagc agcaggccgc cgaggaactc
    15601 gagctcgccg agcgggggca ggccacggtc gaagcgctcg tctagccatc ccggccactc
    15661 cggccgggcg ttggcgtacc atgcgccgat gaagggtttt catgcacgcg tggtcgtgct
    15721 cgctgttctc ggctgcaccg cgctttcgag cgggtgtctg ccgctcccgc cagcggagac
    15781 cgccgccatc tgcaacaccg ctccgtcggg gccaccgacg gccgacgacc tggccgtgct
    15841 cgagcggctg gcggatcgcg acctcccggt ggcgatcgga tcgccgccgt cggttcgtct
    15901 cgacgcgcac gacacagcga cggcgaccgc gttgcgcgcc gagttcgggg cgtccatcga
    15961 catcgagatc ggctcgaagc gactcgggcg ggtgcccggc aacccggatg gctgcctcct
    16021 cgtggcgccg acatgggcga tcgccgagcc ggcgccgaag ggggtcacca tgacggcgac
    16081 gccgacgcag ccggtgttcg accgcaacgg ctccatccga gcgacggtgc gggtcaccaa
    16141 tcgcaccggc cggtcgatcg tggtgtcgca ggcggtcccg aggacgccct cccgcaactg
    16201 cggcgacctc ggcgttgcac tgtcgtcgac gacctcggcc ctcgccgtca atcgcgtcga
    16261 cggcctccag cccctcgatc cgaacttcca actggtccgg accggccgga gctacggtcg
    16321 cctgtcgcga atgggcgacg gctcgttctc gaccgtcatc gtgacgtcca tctgcgcaat
    16381 ccccatcgtg acggggatca ccgtccggcc cggagggtca gccatgatcc cggtcacggc
    16441 tggcaccgag agtgtgcagc ccggcgccga tcccatcgtc ctccccggca cctatcacct
    16501 ccgagcgacc gtcgacgtgc gtctgtcgac cgcgcccgat gccgttgcgc acagggtcgc
    16561 cgctccgccg acgcgggtcg agctgcgcga ctgagcaccc accgacgtcg aacgcgtccc
    16621 agatggtgca agatgtcgtg atgattgctg ggatccctcg ccctcgacgt cgtcgcgcca
    16681 ccctcgccgg ttcgctgctg acgatcgccc tgttcgtctc gtcgtgtgtg ccgcccgcga
    16741 cgctccccga cgggagcgtg ccgcagaccg gcccgatcgg ggccaacggc gtcggcttcg
    16801 acggcaccga cctgtggttg tgcgacctct tcggcggtca gttgctgcgc atcgacccgg
    16861 ggtcgggacg catcctcgag cgcatcgggc ccgaccgggg catcgcccgc cccgacgaca
    16921 tcgtgttcgc gcccgacggg tcgttgatct acacatcgcc gccgaccggc gtcgtcggcc
    16981 gcatcacgag gtccggaccg aacgccggtc aggtgtcggt gctcgcgcag atggagaccg
    17041 gggtgaaccc gatcgccctc acgcccgacg ggtcggccgt gatcgtcggg tacggctccg
    17101 gcgaggtcgg ccgtctcgat cgcatcgatc tcgccaccgg ccaggtgacg gtggtggcc
//
LOCUS       DAOSOA010000024        16714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3472_length_16714_cov_7.425296, whole genome shotgun sequence.
ACCESSION   DAOSOA010000024 DAOSOA010000000
VERSION     DAOSOA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3472_length_16714_cov_7.425296"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            758..1039
                     /locus_tag="PKY13_03115"
     CDS             758..1039
                     /locus_tag="PKY13_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78946.1"
                     /translation="MSTRRTAALATVLVLVNVLAYWATVHSLRGDGFDGLNNSFQLLL
                     GLPWTLTPRGADHVFNALVDLGCGLINGAILVGWIYVRGRKVSGGHPGS"
     gene            complement(1138..1551)
                     /locus_tag="PKY13_03120"
     CDS             complement(1138..1551)
                     /locus_tag="PKY13_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78947.1"
                     /translation="MDLDQARSHRVAEPIAVVVLVSVIWWCAYGRTTPFPSIWRVIPK
                     DDQVGWLFAASALVAVALVAIVAIVPDRRWVGLVGAATWFVLSIWSTRRGDSDGLWAL
                     VIPLYLVVALAIVGLTAMGRALALKLRSRASDFWP"
     gene            1677..3803
                     /locus_tag="PKY13_03125"
     CDS             1677..3803
                     /locus_tag="PKY13_03125"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT78948.1"
                     /translation="MKRVTGVIGRHRVLSGIVVLVAAVGGVFVFRQVEPIVVAQFDDV
                     SFAVPKAPELTAKSGEQLFRIDPKLSKASYSIEEEFAGKSTGTAVGTTQGIAGDLAIN
                     TADPSASRVGEIDINLEQLTSDSSLRDARVRHDYLESSKYPLARFVTTKIDGLPDRIE
                     QGKDVTFTLTGDLNVKTTTKPVTFDVEAVLRGDELQATATATIKMSDFGVGPIDVVGL
                     VSTADEMKLTFELVAVDPSTRTVATRVSAPKEEEATGTGPSFAKVVQPILERNCASCH
                     GKGQLGYGDWKLETAGDAADFASGLALVTRTKYMPPWPASDLGVALQHPRNLDQADID
                     AISDWAAAGGKLDVKRATKVSAKPDDSDVVVPRQDQVLTMSTPYQGTGEIKNDYRCFL
                     LDPGFTEPTFMTGYQFLPDERQVLHHVIVYRVSGSARAEADRQDGEDGRPGWPCFVGT
                     GLPSSNPAAGNGDGNGNGLSYMVGGWVPGQRPADFGEGVGMLFEPGDLLVMQIHYHYD
                     DQNLADQSKFAIEVAPSGSKLLPIQVFNPIAPVEIPCADGETGPLCDRATMAQYLRDT
                     YGPIAAGIPPFALRNCKHTPEEFADQTKGTGWTSCDNRIRGAGHIVAVHGHMHELGKS
                     FRMTLNPGTPEEQILLDIPTWNFEWQLNYPLVEPVKVQEGDNLRIECTWDRSLKYTPE
                     PRYLLFSEGTEDEMCFSTYSFIADEP"
     gene            complement(3870..4715)
                     /locus_tag="PKY13_03130"
     CDS             complement(3870..4715)
                     /locus_tag="PKY13_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT78949.1"
                     /translation="MQELSGKVAVVTGAGSGIGRAMALAFAAEGMSVALADIDGGAAD
                     ETAALVAAAAPGVTTLARAVDVSDGPAVDGFADEVFNTRGQADVLCLNAGVFVGGVIW
                     EVPAGEIDDILGVNLHGILNGARSFLPRMIAQDTEGHVVTTASVAGLFGSPFSGPYGI
                     SKFAAFAAGETIAHDLAATGSKLRASVLCPGIVKTDIANKAARRDSRPGATPSEAQQF
                     VNGVLVDTVDGHGIDPSLVADRVVAGIRSEQFLILTHDQHADAICDRAETLANLELPP
                     IVDYT"
     gene            4848..5471
                     /locus_tag="PKY13_03135"
     CDS             4848..5471
                     /locus_tag="PKY13_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT78950.1"
                     /translation="MSSSAPAPSSTASSTSSSTGSPAAGPVLSARQLDTVNKLVDAAV
                     EEVRAVGFGGLTIRNVAARAGVAPATAYTYFGSKEHLVSEVFWRRLKALPHATVDGRV
                     APPKRVGAMLRDVAMLVGNEPELSAACTIALLVDEPAVKRLRDRIGGNIHQRLVEALG
                     DDDPEIIGALELAYSGAMLQTGTGHLSYDDLGDRLAGAANLILGGRR"
     gene            5468..6667
                     /locus_tag="PKY13_03140"
     CDS             5468..6667
                     /locus_tag="PKY13_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT78951.1"
                     /translation="MSASTGAAHYSPYDYEVHEDPYPFYARLRTEAPLYRNEELGFWA
                     LSRHADVSAGFRDNARFSSAEGVSLDKLASGPHAHKTMSFLAMDPPRHTRMRSLVARG
                     FTPRRVVELEPRIREIAREYLDRAKDLDEFDIIGDFAGKLPMDVISEMMGVPVADRDE
                     VRRLADLLVHREEGVEDVPPAGIEAAITLFGYYTDMIAQRRRQPTDDLTSALLVAEID
                     GDRLDDEDIVAFLFLMVVAGNETTTKLLGHCLYWAWANPAEREKVWSGSHTIEQWIEE
                     TLRYDTSSQLLARTTLVDVELHGGMIPKGDRVVFLVGSANRDPEVFVDPDRYDIGRDT
                     TQLVSFGLGRHFCMGASLARLEAKVALEEFIARFAGYEIDGERSRRVHSTNVRGFATL
                     PTTLAVR"
     gene            6667..7488
                     /locus_tag="PKY13_03145"
     CDS             6667..7488
                     /locus_tag="PKY13_03145"
                     /inference="COORDINATES: protein motif:HMM:NF005854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT78952.1"
                     /translation="MANEFPSHPDRRPAIVTGASSGIGAATATALSAAGFPVVLAARR
                     VDRLEELAKVIRANDGEVAVVRLDVTDDESVGAAFAAAQQAMGDIEIVVSNAGDMVPA
                     PAESADTATFLRQLDTNLVGAHRLVIEGLAPMIARRRGDIVLVTSQNAATPRPLVAAY
                     NSSKAGLESFGRTLQMELEGTGVRASIVRPGPTFTEMGWSWAPDLIDRCLKDWKRWGL
                     LRHHQYLPAESIAAAVVNVVAAPRGTHLSLVEVNPEAPIREAASPESGHESGESA"
     gene            7485..8873
                     /locus_tag="PKY13_03150"
     CDS             7485..8873
                     /locus_tag="PKY13_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT78953.1"
                     /translation="MTVTDATTALPTPPLVSGVGEDGSHLEELRTDPIGLMERVRAEC
                     GDVGSFLLADRPVALLSGAEANEAYFRAPEEELDQAEAYPFMKPVFGEGVVFDASAER
                     RREMMHNQALRDKFMRGHAEKIAAEIDRMVERFGDRGEFEMLDWFAELTIYTSTTCLI
                     GPKFREDLTAEFAELFHDLEKGTDALAFVDPYLDIPSFHRRDAARAELVELIDAILQK
                     RLAQPAPEGDDRDLVDVLLSLRNEDGSLMFSADYVTGMFISMMFAGHHTTSTTLSWTL
                     VELLRNPEAMAAVVEELDELYSDGRSASYQALREMPRTEAAIKEALRLHPPLILLLRV
                     AKVPFEVCGHVVPEGWLVGATPAVSNRIDSVFPDADRYDPTRYLDDRHEDLDNPWNWI
                     PFGAGRHRCVGAQFAMMQLKVILSVLLKDWTFELGQPADSYVNDHSKMVVQMNRPTTV
                     KYHRRTAAEGAR"
     gene            8873..9103
                     /locus_tag="PKY13_03155"
     CDS             8873..9103
                     /locus_tag="PKY13_03155"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOT78954.1"
                     /translation="MGLRIELDEVLCQGHGVCEGEAPDVFSLSKKGELTILQPNPDES
                     LRPQVEAAVKYCPTHALLIFADDSVAGPDKEK"
     gene            9111..9665
                     /locus_tag="PKY13_03160"
     CDS             9111..9665
                     /locus_tag="PKY13_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT78955.1"
                     /translation="MPAFPRSELEEMVERWLQVNREAEAKGDWTDMIAMYTPDATYGW
                     NYGPNEDFMTVGRDEIRDVALGLEMGGLDGWEYPYQKVLIDDQQGEVLGFWKQIATTP
                     RPDGGTYEVAGFGGSWFRYAGSFQWSWQRDFFDVGNATALFIQMFRDDTLSDGMKRRL
                     DVASSGAPQPCRYPVGQAPVGLWD"
     gene            9678..11180
                     /locus_tag="PKY13_03165"
     CDS             9678..11180
                     /locus_tag="PKY13_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT78956.1"
                     /translation="MALPGTPGSDAWGSAEALLLIDGKLVPASGGATYENINPATGKV
                     VGVAPDASLDDIELAMAAARRAFDETDWSTDVEFRVRCIRQLQAAFQEHADEIRELTV
                     AEVGCPVSLTYGAQLDVPVEGLGFAADTAEGYAWETDMGEAAPFGMKSHRTIRREPIG
                     VVAAITPWNFPHQINLAKVGPALAAGCTVVLKAAPATPWSATVLGRLVAEHTDIPAGV
                     LNVITSQQNAIGEVLCTDPRIDLVSFTGSTATGRRIMAAASDTLKKTFLELGGKSAYI
                     VLDDADPATAAIGAAFQIMAHAGQGCAITTRMVVPRAMHDEIVDQVVGMIGGIGWGDP
                     TDAGNFMGPLVSKAQQDRVLGYINTAVEEGATIVTGGKAGALEGDLAGGFFVEPTVLT
                     GVDQNSTIAQEEVFGPVLAVIPHDGDDDAVAIANNSAFGLSGAVVGGDSARARAVANR
                     VRTGTIAVNGGIFYSPDMPFGGYKQSGIGREMGVAGFEEYLEIKSIAEGI"
     gene            11180..12028
                     /locus_tag="PKY13_03170"
     CDS             11180..12028
                     /locus_tag="PKY13_03170"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT78957.1"
                     /translation="MAVAGFIGLGNIGGPMARRLLANPDGLVVFDVRPESYEKLVAKG
                     ATAAADVADVARTADVISVVVRDDDQVRDVTTELLAASRPGLVIAIHSTIKASTAIAL
                     AEVAEPLGVHIVDAPISGGAMGAHAGTLAVMVGGEADAVELCRPVFGHFASLVSHLGP
                     VGSGTRAKLARNLLHFVAFTAVGEAQRLAEAAGIPVAELGKIVRHSDSVTGGPGAIML
                     RDSAGPLAPGDDWYPVMENVASLGAKDLQQALELAAELGVDTPLASMALQRLALELGL
                     PEGSGT"
     gene            12025..12435
                     /locus_tag="PKY13_03175"
     CDS             12025..12435
                     /locus_tag="PKY13_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOT78958.1"
                     /translation="MTDLPEMSEARAKGLEKMTEVYGWEVSDGPGDFFGYTVDHLFAE
                     IWNRPGLSNRDRRLLLVGLCIGSGQEDVLDIQIPAAMGNGELDDEQLREIVIFMAYYA
                     GWPRAARLNNQVEAIIGRRNKAAAKAAAAATESD"
     gene            12439..13209
                     /locus_tag="PKY13_03180"
     CDS             12439..13209
                     /locus_tag="PKY13_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT78959.1"
                     /translation="MGELDGGKFDGKVSIITGAGQGIGEGYAHALAREGASVVVADIN
                     DENAANVVAAIEAEGGTALAVHVDVSDPDSCIAMAAATVERFGRIDHLVNNAAIYHSM
                     EINGLLRIDLDYYRKFMDVNMNGAIYATRAVWRELRKQEGAAVVNQSSTAAWMAAGFY
                     GVAKAALNSITINLAAEMASMGIRVNAIAPGPTNTEATQLISGGEENFGVLLGDVMIK
                     RPGTPEDMANMLIFLLSDDSSWITGQIFAVDGGQSKRI"
     gene            13364..14491
                     /locus_tag="PKY13_03185"
     CDS             13364..14491
                     /locus_tag="PKY13_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HOT78960.1"
                     /translation="MRKSAAALLVAFVIVVGSSCANDASSAESKPDTTTTAPAAETAP
                     AEVADAGPEWRKVATTPNCECSDGSEFHFWVHPGTSERVVFYLEGGGACFSAETCGPT
                     TPSYTRNLQKDTGDSPADSGIFDLANDKNPFKDDSIVFVPYCTGDLHLGNATHDYGGG
                     VVVEHNGASNGGTALTATAALFPDAAEVVVAGSSAGSAPTPVYAGLAQDLFPDAKVTS
                     IADASGAYPGTEAITLAVSAFWGTNAALPPWPETAGDPQVAWSLPGLYVQTTKHAPDV
                     TFAKIDTAYDETQQSFVELAGFPGDDLMTMIDDNTKQAEDGGAKIHSWVGPGEVHTIL
                     GRPELYTTEVEGNSLLEWITALVDGDEVPTVHCTGDCGKPA"
     gene            complement(14501..15214)
                     /locus_tag="PKY13_03190"
     CDS             complement(14501..15214)
                     /locus_tag="PKY13_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78961.1"
                     /translation="MKVLVLSPHYDDAPLSLGQSMLDGELSRHRVTVGVPFGRSNWTI
                     WFHPKRWRWPIASAIRLGEEARNAWRFRYRVRIGGLEEAVLRTGNLSSEGFLDPTFAA
                     GESGDLDSVTELVQKWVSGYDVVVAPLGIGDHVDHQLVAEAGRRLMDAGTAVAFYEDR
                     PYAVLLEESELAAAAKKIDPRLVRRPVSGPMQRNKHERIWYPSQFDEFFLTAIDLDET
                     GARREHVWVLPDSPWPAVG"
     assembly_gap    15343..15442
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15443..16123
                     /locus_tag="PKY13_03195"
     CDS             <15443..16123
                     /locus_tag="PKY13_03195"
                     /inference="COORDINATES: protein motif:HMM:NF040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78962.1"
                     /translation="AKEGGASFYTSGIDPGFGNAGITVHALALCKEVRTVRMMEIVNY
                     ATWDNPFTMFEIMGFGKSDPSQSLLLSPGSTALAWGPVIEMVAAALGVELDGIEEWHE
                     VIRADEDFDIASGTVGAGTISGMRFEIRGMVGGEARIVVEHVTRLRDEDAPDWPQGEG
                     YRIQITGEPHVHLELSVSSDLGDHNHAGCLATAMHVINALPHVIEAEPGVLTTLDLPV
                     YSARHLMV"
     gene            16185..>16714
                     /locus_tag="PKY13_03200"
     CDS             16185..>16714
                     /locus_tag="PKY13_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78963.1"
                     /translation="MTESSDQDASALPERHLTDLVERFSSRQLQYLVGGLAVLAAIVA
                     WGFRFVQDDAFITFRYSRNFANGEGLVLNHGEKVEGYTNFLWTWLMAIPERQGWSTPT
                     FSIVVGIALMIVTIFVANKLARLVMANDRLAFLATVVLVANMSFVGYGTGGLETMLQT
                     LLVTSVAALLLETPGRT"
BASE COUNT         2731 a         5751 c         5586 g         2546 t
ORIGIN      
        1 gcggtggctt cctcgaggcc gacggcgaga tctgggaccg aaccggtcgg ctcgtcgcgc
       61 agtcacgcca gctcgcactc gtgccgcgca gctgacggcc cacgtcggct gttgatcgcg
      121 catgggggtt gatgatcgag catggctggt gacgtgctca tcgaagggcg ttgagagcgc
      181 tctcgacgag ctgaacactg cccttgggta cggcgagttc gagtagcccg gaagcgctcc
      241 gaacttcgac gacgctgaac aggttcttct tgatgttcca ctcgcccgga ccgccgactg
      301 gcctgactcg aaccagggtc agcggaggta ccagggggaa acgctgtcct cggccgtaca
      361 tcccctgaca ccacgtcatc tgcgacccga ccaggtgcag gatgccttgt cggaatcgaa
      421 gtggtagcgg cgcgctctcc gagcgaagca gacaggccac agctcgaccg tccaggagag
      481 cgtctgtcga ctgcggctca gttgagcgcg aacgccgcag aagcacatcc agcatggtcc
      541 gcgtcccagc gtcaggaggg ggagtcgatg cgctcatcac gggagtatgc caacgatctg
      601 agccgtacgg tcgcctgtca gatcgaagtc gcgacccgct ccgcacatgg gtacctccac
      661 ctttgcctcg gtcggagtga tcagtgcgca atgcgcacac gggcggtcga atcgccgcgg
      721 cacaccggcg ccaccatctc ggtcataggt tgaggcgatg agcacccgga gaacggccgc
      781 cctggcgaca gtgctcgtgt tggtgaatgt gctcgcgtac tgggcgaccg ttcacagtct
      841 ccgcggcgac ggtttcgacg gactgaacaa ctcgttccag ctgctgctcg ggctgccgtg
      901 gacgctcacc ccacgcggtg ccgaccacgt cttcaatgcg cttgttgacc tggggtgcgg
      961 gctcatcaac ggggcgatcc tggtcggctg gatctatgta cgcgggcgga aggtgagcgg
     1021 cgggcaccct ggatcgtgac gcactcgcgg gaggtgagtg cgggctcgac gagccgcaca
     1081 cttcccttgg gcgcggcgag ctcgagtagc ccgcgggcgc tcttcacgtc gcctgggtca
     1141 gggccagaag tccgacgctc tcgaccggag cttgagggcg agcgctcgac ccatcgccgt
     1201 caacccgacg atcgccaacg cgacgaccag atagagcggg atgaccagcg cccagagtcc
     1261 gtcgctgtcc ccgcgtcggg tggaccagat gctgaggacg aaccaggtcg cagcaccaac
     1321 gaggccgacc caccgccggt cggggacgat ggcgacgatg gccacgagtg cgaccgcaac
     1381 cagcgctgag gcggcgaaga gccagccgac ctgatcgtcc ttcggaatga cccgccagat
     1441 cgatgggaat ggtgtggtcc ggccatacgc gcaccaccag atcacgctca ccagaacgac
     1501 gaccgcgatc ggttcggcga cccggtgcga gcgggcctga tcgagatcca cgctgtcaag
     1561 gtacgccgcc ttgcggcaga gcgacgccga ggtcattcca tcgtctcgca acgcgttgcc
     1621 tctgtggtca cgggttgcca gcgccctcgc cggcaccgca cgggtacgct tccgacatga
     1681 agagggtgac gggggtcatc gggcggcatc gagtgctgtc cggaatcgtg gtgttggtcg
     1741 cggccgtggg cggcgtgttc gtgttccgtc aggtggagcc gatcgtcgtg gcgcagttcg
     1801 acgacgtgtc gtttgccgtg cccaaggcgc cggagctcac ggcgaagtcg ggcgagcagt
     1861 tgttccgcat cgacccgaag ctgtccaagg cctcgtactc gatcgaggag gagttcgccg
     1921 ggaagtcgac cggcacggcc gtcggcacga cgcagggcat tgccggcgac ctcgccatca
     1981 acaccgccga cccgtcggcg agccgcgtcg gtgagatcga catcaacctc gagcagctca
     2041 cgtcggactc gtcgctgcgc gacgctcgcg tccgccacga ctatctcgag tcgtccaagt
     2101 acccgcttgc ccggttcgtc accacgaaga tcgacgggct gcccgatcgc atcgagcaag
     2161 ggaaggacgt caccttcacc ctcacgggcg acctcaacgt gaagacgacg accaagcccg
     2221 tcaccttcga cgtcgaggcc gtcctgcgtg gcgacgagct gcaggcgacg gccaccgcga
     2281 cgatcaagat gtccgacttc ggggtggggc cgatcgacgt cgtcgggctg gtgtcgaccg
     2341 cggacgagat gaagctgacg ttcgagctgg tcgccgtcga cccgtcgacc cgcacggtgg
     2401 ccaccagggt gtcggcgccg aaggaggaag aggcaaccgg aaccggtccg tccttcgcca
     2461 aggtcgtgca accgatcctc gagcgcaact gcgcttcctg ccatggcaag ggccagctcg
     2521 gttacggcga ttggaagctc gagacggccg gcgacgccgc cgacttcgcg agtggcctcg
     2581 ccctcgtcac ccgcaccaag tacatgccac cctggccggc gtccgacctg ggtgtcgccc
     2641 tgcaacaccc gcggaacctc gatcaggccg acatcgacgc catcagcgac tgggcggcgg
     2701 ccggcgggaa actcgacgtg aagcgtgcga ccaaggtctc ggccaagcct gacgacagcg
     2761 acgtcgtggt gccccgccag gaccaggtgc tcacgatgag caccccctat cagggcacgg
     2821 gcgagatcaa gaacgactac cgctgcttcc tgctcgaccc cgggttcacc gagccgacct
     2881 tcatgacggg ctaccagttc ctgcctgatg agcggcaggt gctgcaccac gtgatcgtgt
     2941 accgggtgtc aggcagtgcc cgcgctgagg ccgatcgtca ggacggcgag gacggtcgcc
     3001 cgggctggcc gtgtttcgtc ggcaccgggc tgccgtcgag caaccccgca gcgggcaacg
     3061 gcgatggcaa cggcaacggt ctgtcgtaca tggtgggcgg ctgggtgccc ggccaacggc
     3121 cggccgattt cggtgagggc gtcggcatgc tcttcgaacc cggcgatctc ctcgtgatgc
     3181 agatccacta ccactacgac gaccagaacc tcgccgacca gtcgaagttc gcgatcgaag
     3241 tcgccccgag cgggtcgaag ctgcttccga tccaggtgtt caaccccatc gcccccgtcg
     3301 agatcccgtg cgccgacggc gagaccggcc ccctgtgcga ccgcgccacg atggcgcagt
     3361 acctccgcga tacctacggc ccgatcgctg ccggcatccc gccgttcgcg ctgcgcaact
     3421 gcaagcacac gcccgaggag ttcgccgatc agaccaaggg aaccggctgg acgtcgtgcg
     3481 acaaccgcat ccgcggggcc gggcacatcg tcgccgtgca cggccacatg cacgagctcg
     3541 gcaagtcgtt ccgcatgacg ctcaaccccg gtacgcccga ggagcagatc ctgctcgaca
     3601 tcccgacgtg gaacttcgaa tggcagctca actacccgct ggtcgagccg gtgaaggtgc
     3661 aggagggcga caacctgcgc atcgagtgca cgtgggaccg ctcgctgaag tacacacccg
     3721 agccgcggta cctgctgttc agcgagggca ccgaggacga gatgtgcttc tcgacctact
     3781 cgttcatcgc cgacgaaccc tgaccgcagg ggctttgtac tgcgaggaat cccgttcgtg
     3841 gcgcgcctcg cagtacaaag cccccagggt cacgtgtagt ccacgatggg cggcagttcg
     3901 aggttcgcga gggtctcggc gcggtcacag atcgcgtcgg cgtgttggtc gtgggtgagg
     3961 atcaggaact gctcgctccg aatgccggcg acgacacggt cggcgaccag ggacggatcg
     4021 atgccgtgcc cgtccacggt gtcgacgagg acgccgttga cgaactgctg agcctcgctc
     4081 ggcgtagcgc ccgggcggga atcgcgccgg gccgccttgt tggcgatgtc ggtcttgacg
     4141 atgcccgggc acagcaccga tgcccgcagc ttcgatccgg tggcagccag gtcgtgggcg
     4201 atggtctcgc cggcggcgaa cgcggcgaac ttcgagatgc cgtacggacc ggagaacggt
     4261 gagccgaaca ggccggcgac cgaggccgtc gtcacaacgt gaccctcggt gtcctgggcg
     4321 atcatgcgcg gcaggaacga gcgggcgccg ttgaggatgc cgtgcaggtt cacccccagg
     4381 atgtcgtcga tctcgccggc gggcacctcc cagatgaccc cgccgacgaa cacgccggcg
     4441 ttgaggcaca gcacgtcggc ctgtccccgc gtgttgaaca cctcgtcggc gaacccgtcg
     4501 accgccgggc cgtcgctcac atcgacggcc cgggcgagcg tggtcacccc gggcgctgcc
     4561 gcggccacga gcgcagcggt ctcatcggcg gcaccgccgt cgatgtcggc gagtgccacc
     4621 gacatgccct ccgccgcgaa cgccagggcc atcgcccgac cgatgccgct cccggcgccg
     4681 gtcacgaccg ccaccttgcc agacagttcc tgcacgctgg acccccgtcc ccactcgatt
     4741 tgtcccgcgg atggatgcag ttgccgttcg ttcgcgggac agaacgggag cgtagcgggg
     4801 cggactcctt gccaactgga caggtgtccg gttatcgtcc tggacacatg tccagttcgg
     4861 cgcccgcccc cagctcgacc gccagttcga ccagcagttc gaccggcagt ccggccgctg
     4921 gtccggtgct gtcggcgcgt cagctcgaca cggtcaacaa gctcgttgac gccgccgtcg
     4981 aagaggtgcg ggccgtcggc ttcggcggac tcacgatccg caacgtggca gcacgcgccg
     5041 gcgttgcgcc cgccaccgcc tacacctact tcggctcgaa ggaacacctc gtcagcgagg
     5101 tgttctggcg acgcctcaag gcattgccgc acgcaacggt cgacggccgc gtcgccccac
     5161 ccaagcgcgt cggcgcgatg ctgcgcgacg tcgccatgct cgtcggcaac gagcccgagc
     5221 tgtcggcggc gtgcacgatc gcgctgctgg tcgacgagcc cgccgtgaag cggctgcgcg
     5281 atcgcatcgg cggcaacatc caccagcgcc tcgtcgaggc gctcggcgac gacgaccccg
     5341 agatcatcgg ggcgctcgag ctcgcctact ccggcgcgat gctgcagacc ggcaccggcc
     5401 atctgtccta cgacgacctc ggcgaccgcc tcgccggggc cgccaacctc atcctgggag
     5461 gccgccgatg agcgcgagca ccggggctgc gcactacagc ccctacgact acgaggtcca
     5521 cgaggatccg tacccgttct atgcgcggct gcggaccgag gcgccgctgt accgcaatga
     5581 ggagctcggc ttctgggcgc tgtcgcgcca cgccgatgtg tcggccggct tccgtgacaa
     5641 tgcgaggttc tcgtcggcgg agggcgtgtc gctcgacaag ctggcgtcgg gcccgcacgc
     5701 ccacaagacc atgtcgttcc tcgcgatgga tccgccgcgg cacacccgca tgcggtcact
     5761 cgtggcccga ggcttcacgc cccgccgcgt cgtcgagctc gagccccgca tccgcgagat
     5821 cgcccgggag tacctcgacc gggcgaagga cctcgacgag ttcgacatca tcggcgactt
     5881 cgccggcaag ctcccgatgg acgtcatcag cgagatgatg ggcgttcccg tcgccgaccg
     5941 cgacgaggtg cgacgcctcg ccgacctgct cgtgcaccgc gaagaaggcg tcgaggacgt
     6001 cccgcccgcc ggcatcgaag cggccatcac gctgttcggg tactacaccg acatgatcgc
     6061 ccagcggcgc cgccagccca ccgacgacct tacgtcggcg ctgctcgtgg ccgagatcga
     6121 cggcgatcga ctcgacgacg aggacatcgt ggcgttcctg ttcctgatgg tcgttgccgg
     6181 caacgagacg acgaccaagt tgctcggcca ttgcctctac tgggcctggg ccaacccggc
     6241 cgaacgcgag aaggtgtggt cgggttcgca caccatcgag caatggatcg aggagacgct
     6301 gcgctacgac acgtcgtcgc agctcctcgc ccgcaccacg ctggtcgatg tcgagctgca
     6361 cggcgggatg atccccaagg gtgaccgcgt cgtgttcctc gtcggatcgg ccaaccgcga
     6421 tcccgaggtg ttcgtcgacc ccgaccgcta cgacatcggg cgcgacacga cccagctcgt
     6481 gtcgttcggt ctcggccgcc acttctgcat gggcgcctcg ctggcgcggc tcgaggcgaa
     6541 ggtcgcgctc gaggagttca tcgcccggtt cgccgggtac gagatcgacg gcgagcgttc
     6601 gaggcgggtg cactccacga acgtccgagg cttcgccacc ctcccgacca cgctggcggt
     6661 ccgctgatgg cgaacgagtt cccctcccac cccgatcggc gccccgccat cgtcaccgga
     6721 gcgtcgtcgg gcatcggcgc ggcgaccgcg accgcactgt ccgctgccgg tttcccggtg
     6781 gtcctcgctg ctcgccgtgt cgaccggctc gaggagctgg ccaaggtcat ccgcgccaac
     6841 gacggcgagg tcgcggtcgt gcgcctcgac gtgaccgacg acgagtcggt cggcgccgcg
     6901 ttcgccgcgg cgcagcaggc gatgggcgac atcgagatcg tggtgtccaa tgccggtgac
     6961 atggttccgg ctcccgccga gtcggccgac accgccacgt tcctccgaca gctcgacacc
     7021 aacctcgtcg gggcgcaccg tctggtcatc gaaggcttgg ccccgatgat cgcccgacgc
     7081 cgcggcgaca tcgtcctcgt cacctcgcag aacgcggcca caccccgacc cctggtcgcg
     7141 gcgtacaact cctcgaaggc cggcctcgag tcgttcggac ggaccctcca gatggagctc
     7201 gaaggcaccg gtgtacgggc ctcgatcgtt cgtcccgggc cgacgttcac cgagatgggc
     7261 tggagctggg cgcccgacct gatcgaccgt tgcctcaagg actggaagcg ctggggactc
     7321 ctccgccacc accagtacct cccggccgag tccatcgccg ctgccgtcgt caacgtggtg
     7381 gccgccccgc gtggcaccca cctctcgctt gtcgaagtga atcccgaagc cccgatccgc
     7441 gaggccgcct cgcccgagtc gggtcacgaa tcaggagagt cagcatgacc gtcactgatg
     7501 ccaccactgc cctgcccacc ccgccactgg tgtccggtgt cggcgaggac ggcagccatc
     7561 tcgaggagct ccgcaccgac cccatcggcc tcatggaacg cgtccgcgcc gagtgcggcg
     7621 acgtcgggag cttcctcctc gccgatcgcc ccgtcgcgct gttgtcgggc gccgaggcca
     7681 acgaggccta cttccgtgcg cccgaggagg agctcgacca ggccgaggcg tatcccttca
     7741 tgaagcccgt cttcggggag ggtgtggtct tcgacgcgtc cgcggaacga cggcgcgaga
     7801 tgatgcacaa ccaggcgttg cgcgacaagt tcatgcgcgg ccatgccgag aagatcgccg
     7861 ccgagatcga ccggatggtc gagcggttcg gcgaccgtgg cgagttcgag atgctcgatt
     7921 ggttcgcgga gctgaccatc tacacgtcca cgacgtgtct catcggcccc aagttccgtg
     7981 aggacctgac ggcggagttc gccgagctgt tccacgacct ggagaagggc accgacgcgc
     8041 tcgccttcgt cgacccctac ctcgacatcc cgtcgttcca ccgccgcgac gccgcccgcg
     8101 ccgaactggt cgagctcatc gacgcgatcc tccagaagcg tcttgcccag ccggcaccgg
     8161 agggcgacga tcgcgatctc gtcgacgtgt tgctgtcgct ccgcaacgag gacggctcgc
     8221 tgatgttcag cgccgattac gtgacgggca tgttcatctc gatgatgttc gccgggcatc
     8281 acaccacgtc gacgacgctg tcgtggacgc tcgtcgagct gctccgcaac cccgaggcga
     8341 tggccgcggt ggtcgaggaa ctggacgagc tgtactccga cggccgctcg gcgtcctacc
     8401 aggcgctgcg cgagatgccc cgaaccgagg cggccatcaa ggaggcgctg cggctccatc
     8461 cgccgctgat cctgctgctc cgtgtcgcca aggtgccgtt cgaggtgtgc ggccacgtcg
     8521 tgccggaggg ctggctcgtc ggcgcgaccc ccgcggtgtc gaaccggatc gactcggtgt
     8581 tccccgacgc cgaccggtac gaccccaccc gctacctcga cgatcgccac gaggatctcg
     8641 acaacccgtg gaactggatc ccgttcgggg ccggtcgcca ccggtgcgtg ggggcccagt
     8701 tcgcgatgat gcagctcaag gtcatcctgt cggtgctcct caaggactgg acgttcgagc
     8761 tgggccagcc ggccgacagc tacgtcaacg accactcgaa gatggtggtg cagatgaacc
     8821 ggccgaccac cgtgaagtac caccgccgca cggccgcgga aggtgcccgc tgatgggcct
     8881 gcgcatcgag ctcgacgagg tgttgtgcca gggacacggc gtctgcgagg gcgaggcacc
     8941 cgacgtgttc tcgctctcga agaaggggga gttgacgatc ctccaaccca atcccgacga
     9001 gtcgctgcgg ccccaggtcg aggccgccgt gaagtactgc ccgacccatg ccctgctgat
     9061 cttcgccgac gactccgtcg ccggacccga caaggagaag tgatccaaat atgcccgcat
     9121 tcccgcgatc cgaactcgag gagatggtgg agcgttggct ccaggtcaat cgcgaggccg
     9181 aggccaaggg cgactggacc gacatgatcg ccatgtacac gcccgacgcc acgtacggct
     9241 ggaactacgg gcccaacgag gacttcatga cggtcgggcg cgacgagatc cgcgacgtcg
     9301 ccctcggtct cgagatgggt ggcctcgacg ggtgggagta cccgtaccag aaggtgctga
     9361 tcgacgatca gcagggtgag gtgctcggct tctggaagca gatcgcgacg acaccgcgtc
     9421 ccgacggcgg cacctacgag gtcgccgggt tcggtgggag ctggttccgc tacgccggca
     9481 gcttccagtg gagctggcag cgcgacttct tcgacgtcgg caacgcgacg gccctcttca
     9541 tccagatgtt ccgcgacgac accctgtcgg acggcatgaa gcgtcggctc gacgtcgcca
     9601 gctcgggcgc accgcagccg tgccgctacc ccgtgggcca agcgcccgtc gggctgtggg
     9661 actgagagag gactgagatg gcactcccag gcacacccgg tagcgacgcg tggggctcgg
     9721 ccgaagcgct cctgctaatc gacggcaagc tggtgcccgc gtccggcggc gccacctacg
     9781 agaacatcaa cccggcgacc ggcaaggtcg tcggcgtggc gcccgacgcg tcgctcgacg
     9841 acatcgagct cgccatggcc gccgcccgcc gagccttcga cgagaccgac tggtcgaccg
     9901 acgtcgagtt ccgggtgcgc tgcatccgtc agctccaggc ggcgttccag gagcacgccg
     9961 atgagatccg tgagctgacc gtcgccgagg tcggctgtcc cgtctcgctc acctacggcg
    10021 cccagctcga cgtgcccgtc gaaggcctcg gcttcgctgc cgacacggcc gagggctacg
    10081 cgtgggagac cgacatgggc gaggcggccc cgttcggcat gaagtcgcac cgcacgatcc
    10141 gccgcgagcc catcggcgtg gtcgcggcga tcaccccgtg gaacttcccc caccagatca
    10201 acctcgccaa ggtcggtccg gcgctcgccg ccggctgcac cgtcgtgctc aaggcagcgc
    10261 ctgcgacgcc gtggtcggcc acggtgctcg gccgtctggt cgccgagcac accgacattc
    10321 ctgccggcgt gctcaacgtg atcacgtcgc agcagaacgc catcggcgag gtgctgtgca
    10381 ccgatccccg gatcgatctc gtgtcgttca cggggtcgac cgcaaccggt cgccggatca
    10441 tggccgcggc gtccgacaca ctcaagaaga cgttcctcga gctgggcggc aagtcggcgt
    10501 acatcgtctt ggacgacgcc gaccccgcca cggcagcgat cggtgcagcg ttccagatca
    10561 tggcgcacgc cggtcagggc tgcgcgatca cgacccgcat ggtggtccct cgggcgatgc
    10621 acgacgagat cgtcgatcag gtcgttggca tgatcggcgg tatcgggtgg ggcgacccga
    10681 ccgatgccgg caacttcatg gggccgctcg tgagcaaggc ccagcaggat cgggtgctcg
    10741 gctacatcaa cactgcggtc gaggagggcg ccacgattgt caccggcggc aaggccgggg
    10801 ccctcgaggg cgacctcgcc ggcggcttct tcgtggagcc gacggtcctg acgggtgtcg
    10861 accagaactc gacgatcgcc caggaagagg tgttcggccc ggtgctcgcc gtcattcccc
    10921 acgacgggga cgacgacgcc gtcgccatcg ccaacaactc ggccttcggc ctgtccggtg
    10981 cggtcgtcgg tggcgacagt gctcgggcgc gggccgtggc caaccgcgtg cgcaccggca
    11041 cgatcgccgt gaacggcggc atcttctaca gccccgacat gcccttcggc ggctacaagc
    11101 agagtggcat cggccgcgag atgggtgtcg ccgggttcga ggagtacctc gagatcaagt
    11161 cgatcgccga gggcatctga tggcggtcgc cgggttcatc ggcctgggca acatcggcgg
    11221 gccgatggca cggaggttgc tcgccaaccc cgacggcctc gtcgtgttcg acgttcgccc
    11281 cgagtcgtac gagaagctgg tcgccaaggg tgcgaccgcg gccgccgacg tggccgacgt
    11341 cgcccgaacc gccgacgtga tctcggtggt cgtgcgcgac gacgaccagg tgcgcgacgt
    11401 cacgaccgag ctcctcgccg cgtcgcggcc gggcctggtc atcgccatcc actcgacgat
    11461 caaggcgtcg acggcgatcg ccctcgccga ggtcgccgag ccgctcgggg tccacatcgt
    11521 ggacgccccg atctccggcg gtgccatggg cgcccacgcc ggcaccctcg ccgtgatggt
    11581 cggtggcgag gcggacgcgg tcgagctgtg ccgccccgtg ttcggccact tcgcctccct
    11641 cgtcagccat ctcgggccgg tcggttccgg cacccgcgcc aagctggcgc gcaacctgtt
    11701 gcacttcgtg gccttcaccg ccgtcggcga ggcccagcgc ctggccgagg cggccggcat
    11761 cccggtcgca gagctgggca agatcgtgcg gcacagcgac tcggtgacgg gcgggccggg
    11821 ggcgatcatg ttgcgcgact cggccggtcc actcgcaccg ggcgatgact ggtaccccgt
    11881 gatggagaac gtggcgtcgc tcggcgccaa ggacctgcag caggcgctcg agctcgccgc
    11941 cgagctcggg gtggacaccc ccctcgcgtc gatggcgctg caacgactgg cgctcgagct
    12001 cgggctgccc gaagggagcg gaacatgacc gacctgcccg agatgtccga ggcgcgagcc
    12061 aagggcctcg agaagatgac cgaggtctac ggctgggagg tgagcgacgg acctggcgac
    12121 ttcttcgggt acaccgtcga ccacctgttc gcggagatct ggaaccggcc cggtctctcc
    12181 aaccgcgatc ggcgacttct cctcgtgggc ctgtgcatcg gatcgggtca agaggacgtc
    12241 ctcgacatcc agatccccgc agccatgggc aacggcgagc tcgacgacga gcagctccgg
    12301 gagatcgtga tcttcatggc gtactacgcc ggttggcccc gcgccgctcg gctgaacaat
    12361 caggtggagg ccatcatcgg ccgccgcaac aaggctgcag cgaaggccgc tgccgccgcg
    12421 acagaaagcg actgatcaat gggtgagttg gacggcggca agttcgacgg caaggtgtcg
    12481 atcatcacgg gtgccgggca gggcatcggc gaggggtacg cgcacgcgct ggcccgtgag
    12541 ggtgcgtcgg tcgtcgtggc cgacatcaac gacgagaacg cggcgaacgt cgtcgctgcg
    12601 atcgaggccg agggcggaac ggcactggcc gtgcacgtcg acgtgtccga tcccgacagc
    12661 tgcatcgcca tggcggcggc caccgtcgag cggttcggtc gcatcgatca cctcgtcaac
    12721 aacgcggcga tctaccactc gatggagatc aacggactgc tgcggatcga cctcgactac
    12781 taccgcaagt tcatggacgt gaacatgaac ggggcgatct acgccacccg tgccgtctgg
    12841 cgcgagctgc gcaagcagga gggcgcggcg gtcgtgaacc agtcgtcgac ggcggcctgg
    12901 atggcggcgg gcttctacgg tgtcgcgaag gcggcgctca actcgatcac gatcaacctg
    12961 gcggccgaga tggcctcgat ggggatccgc gtcaacgcca ttgcacccgg cccgaccaac
    13021 accgaggcca cgcagctgat cagcggcggc gaggagaact tcggtgtcct cctcggcgac
    13081 gtgatgatca agcgcccggg cacgcccgag gacatggcca acatgctgat cttcctgctg
    13141 tccgacgaca gctcgtggat caccggccag atcttcgccg tcgacggcgg ccagtcgaag
    13201 cgcatctgag gtccgaggcg ccagccgacc gttctggcaa tccaggctca gccgggcaca
    13261 tccgcccggc tgagcctgga tcgcgccgtg cctcgtgagt gctgcgctgc gggctagctt
    13321 cggagtcggg cggcgatcgg cgcccgactc agggggagta cacatgcgga agtccgctgc
    13381 agcgttgctc gttgcgttcg tgatcgtcgt cggctcgtcg tgcgcgaacg acgcgtcgtc
    13441 ggccgaatcc aagcccgaca ccaccacgac ggcgccggcc gccgagaccg cgccggccga
    13501 ggtggccgac gccgggccgg agtggcgcaa ggtcgcgacc acgccgaact gcgagtgcag
    13561 cgacggttcg gagttccact tctgggtgca ccccggtacg agcgagcgcg tcgtcttcta
    13621 cctggagggc ggcggcgcgt gcttctccgc cgagacctgt gggccgacca ccccctctta
    13681 cacccgcaac ctccagaagg acactggcga cagcccggcg gactccggca tcttcgacct
    13741 cgccaacgac aagaacccgt tcaaggacga ctcgatcgtg ttcgtcccgt actgcacggg
    13801 cgacctgcac ctcgggaacg cgacccacga ctacggcggg ggagtcgtcg tcgagcacaa
    13861 cggggcgtcc aacggcggca cggcgctcac tgcgaccgcg gccctgttcc ccgatgctgc
    13921 cgaggtcgtc gtcgccggga gcagtgccgg ttccgcgccg acgccggtgt acgccgggct
    13981 ggcgcaagac ctgttccccg acgccaaggt cacctcgatc gccgacgcgt ccggcgccta
    14041 ccccggaacc gaggccatca cgctcgccgt gtcggccttc tgggggacga atgcggcgtt
    14101 gcccccgtgg ccggagaccg ccggcgatcc gcaggtcgcc tggtcgttgc ccgggctcta
    14161 cgtgcagacc acaaagcacg ctcccgacgt cacgttcgcc aagatcgaca cggcctacga
    14221 cgagacgcag cagtcgttcg tggagctcgc cggattcccc ggtgacgacc tcatgacgat
    14281 gatcgacgac aataccaagc aggctgagga cggcggggcg aagatccaca gctgggttgg
    14341 cccgggtgag gtccacacga ttctcggtcg cccggagctc tacacgaccg aggtcgaggg
    14401 caactcgttg ctcgagtgga tcacggcgtt ggtggacggc gacgaggtac cgacggtcca
    14461 ctgcaccggc gactgcggca agcccgcctg agccagtcag tcaccccacc gccggccacg
    14521 gtgagtccgg caggacccac acgtgctcgc ggcgggcacc ggtctcgtcg aggtcgatgg
    14581 cggtcaggaa gaactcgtcg aactggctcg ggtaccagat gcgctcgtgc ttgttgcgtt
    14641 gcatcgggcc gctgacgggc cgccgcacga gccgcgggtc gatcttcttg gcggcggccg
    14701 cgagttcgga ctcctccagg aggacggcgt agggccggtc ctcgtagaac gccacggcgg
    14761 tcccagcgtc catcagccgt cggcccgcct cggccacgag ttgatgatcg acgtggtcgc
    14821 cgatgcccag cggagcgacg accacgtcgt agcccgatac ccatttctgg acgagttcgg
    14881 tcaccgaatc caggtcaccc gactccccgg cggcgaacgt cggatcgagg aagccctcgc
    14941 tcgacaggtt gccggtccgg aggacggcct cttccaggcc gccgatgcgc acgcggtacc
    15001 tgaaccgcca cgcgttgcgg gcctcctcgc cgagccggat ggccgaggcg atcggccagc
    15061 gccagcgctt cgggtggaac cagatcgtcc agttcgaccg gccgaacggc acgccgacgg
    15121 tgacacggtg ccgggacagc tcaccgtcga gcatcgactg gcccagactg aggggcgcat
    15181 cgtcgtagtg cggcgagagc acgagaacct tcaccgaccg gacactactg tccctcccac
    15241 cgaccacccc gggggagaca ccgtgaccga cagcaaaccg ccctatcgag tgatccagtg
    15301 ggcgacgggc acggtgggca tccatgcggt gccggccatc gcnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nngcgaagga gggcggcgcc agcttctaca cgtcgggcat
    15481 cgacccgggc ttcggcaacg ccggcatcac ggtgcacgcg ctcgcgctct gcaaggaagt
    15541 gcggacggtt cgaatgatgg agatcgtcaa ctacgcgacg tgggacaacc ccttcacgat
    15601 gttcgagatc atgggcttcg ggaagtccga tccgagccag tccctgctcc tgtcaccggg
    15661 cagtaccgca ctggcgtggg ggcccgtcat cgagatggtc gccgcagcgc tcggcgtcga
    15721 gctcgacggc atcgaggagt ggcacgaggt catccgggcc gacgaggact tcgacatcgc
    15781 gtcgggcacc gtcggtgccg gcacgatctc gggcatgcgc ttcgagatcc gcggcatggt
    15841 cggcggcgag gcgcgcatcg tcgtcgagca cgtgacgcgg ctccgcgacg aggacgcccc
    15901 cgactggccc cagggcgagg gctatcgcat ccagatcacc ggtgagcctc acgtgcacct
    15961 cgaactgtcg gtgtcgagcg acctcggtga ccacaaccac gcgggctgcc tcgccaccgc
    16021 catgcacgtg atcaacgcgc tgccgcacgt gatcgaggcc gagccgggcg tgctcaccac
    16081 gctggatctg ccggtgtact cggcgcgaca cctcatggtc tgacgatgga atgaacgcgg
    16141 cgacgcgccg ctaacgtcgt ggggtgcgtc gacgagggga gagcgtgacc gagagcagtg
    16201 accaggacgc gtcggcgctg cccgagcgcc atctgaccga tctggtcgag cggttctcgt
    16261 cccgccaact ccagtacctc gtcggcgggc tcgccgtcct cgccgccatc gtggcgtggg
    16321 gcttccggtt cgtgcaggac gacgcgttca tcacgttccg ctactcccgg aacttcgcga
    16381 acggcgaggg gctcgtcctc aaccacggcg agaaggtcga gggctacacc aacttcctgt
    16441 ggacctggtt gatggcgatc cccgagcgac agggatggtc gacgcccacg ttctcgatcg
    16501 tggtgggcat cgccctgatg atcgtcacga tcttcgtcgc caacaagctc gcccgcctcg
    16561 tgatggcgaa cgaccggctc gcgttcctgg caacggtcgt cctcgtcgcc aacatgagct
    16621 tcgtcggtta cggcacgggc ggcctggaga cgatgctgca gacgctgctc gtcacgagcg
    16681 tcgcggccct gctgctcgag acgcccgggc gcac
//
LOCUS       DAOSOA010000025        16588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3515_length_16588_cov_7.510071, whole genome shotgun sequence.
ACCESSION   DAOSOA010000025 DAOSOA010000000
VERSION     DAOSOA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16588
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3515_length_16588_cov_7.510071"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="ligD"
                     /locus_tag="PKY13_03205"
     CDS             complement(<1..974)
                     /gene="ligD"
                     /locus_tag="PKY13_03205"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOT78964.1"
                     /translation="MATKEETTIEVDGREVRISSPSKVFFKERGETKLDLVNYYLAVA
                     EPIMRTIRGRATMLQRFPDGASGKSFYQKRVPDNAPEWLQQTFASTPNGTHSNVLVVA
                     DMAHLIWAVNLGCLGFHPWPYLAATPETTDELRIDLDPTPGQTFDDIRAAGFEVKAFL
                     DEIGIVAYPKTSGNRGLHIYVRLEPRWDSYDVRAAAVAMARELERRRPDQLTAAWWKE
                     ERGNRVFIDFNQNAPHKTVFGAWSVRSRVGGQVSAPLTWDELETVELDDLTLANVPGR
                     VAANGDPWEPMNASPQSIETLLEMSARDMAAGLMDAPWPPVYPKQPNEP"
     assembly_gap    1141..1240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1360..1965
                     /locus_tag="PKY13_03210"
     CDS             1360..1965
                     /locus_tag="PKY13_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78965.1"
                     /translation="MNHERLTSRAPTPTARRLRGPLVGLVASFALIGGACAPQTPPAG
                     GGGIGIDFGPLTIPLPPITIRPPATTIPVGICNIGYQPPGVQIIGATVTIPGVRVDPN
                     NPIITVPNVVVNIPQLRLPLSTVGLNCGLINLTTQVDLLLPSTVVVKAATLNIAARTI
                     TLTDPSFTVNGAGLGIPGLGDLVVPLPPIVAVPLPSGAIAF"
     gene            complement(2083..2436)
                     /locus_tag="PKY13_03215"
     CDS             complement(2083..2436)
                     /locus_tag="PKY13_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT78966.1"
                     /translation="MTTSKGVPTFIGGGAPLQVVCSTASDHQAIAVSGELDRETTPRL
                     ATVLHRAIAEAPERIELDLSGVTFMDSQGLRILLEAKRRAGDDVEIVVTETSAQVERL
                     LHTTGVADVLGLASR"
     gene            2539..4098
                     /locus_tag="PKY13_03220"
     CDS             2539..4098
                     /locus_tag="PKY13_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT78967.1"
                     /translation="MARRRNRRGRRTWPQRLLLLVNSLLVVACLGVAGTFNLVRAKAS
                     ELPTIDIGAEARQRPDATGPRNILLVGTDSGEGLDEDDPVQVGREGENLADVIMILRV
                     DPSNESASLLSIPRDTWVPIAPSWSKSKINSAFGGNDGPNTLIATIKHNFGISIDNYV
                     KVDFKGFRDIIDVLGGIPVYNEHPISDRKTSLFLPETGCIMIDPVQALAYARSRHLRY
                     QTGDTYDPKAKWITDGSSDLGRITRQQDFIRQAAQRAIDEGIKNPTTAYGLINAALGS
                     VEKDDDLSVGEIQDLIVTFRDFSVQELTSEQIPTVSGGNPKISYQLVVWDQAEGLLDI
                     YRGIREPGQVQPSDVIVGLPASVPTSAALSEQLDAIGFDAGTEDSSVKSSGKRPGTVI
                     RYGLRGIEAARVLASWLDGEATYEFDADLPGRRLELLPGTAPLALLETARPLDQVPVP
                     VINERRTGKGTTTTSSTTSTTVPTTSTTATSGSGAPSTTTTTTTTTAPESPTTTAIGF
                     LPLDAERAASC"
     gene            complement(4105..5694)
                     /gene="ilvA"
                     /locus_tag="PKY13_03225"
     CDS             complement(4105..5694)
                     /gene="ilvA"
                     /locus_tag="PKY13_03225"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HOT78968.1"
                     /translation="MTSTDEPSTDEPATGLTPTDYLKRILTAQIGDRAVESSLDAAPR
                     LSERLGNRVLLKREDQQPVFSFKLRGAYNKMANLDAAALDRGVICASAGNHAQGVALS
                     ARELGTRAVVVMPVTTPELKVDAVRALGAEVVLAGESYSDAHEVALVHQRERGLTFVH
                     PFDDPDVIAGQGTVAMELLRQHQGRLDAVFVAVGGGGLISGIANYVKAVRPEIKVIGV
                     QTSDSDAMIRSVDAGHRVELENVGLFSDGTAVRMVGEETFRIVTDLVDDLIRVDTDAV
                     CAAIKDVFLDTRSIVEPAGALGLAGLKAYVADHGTLGETYATILCGANMNFDRLRFVA
                     DRAEVGEEREVLLAVTIPEVPGSFRRFAELVGSLPGGPRNVTEFNYRLSGRSDAHVFV
                     GLTTSVRGESTTIADRFRAAGFATIDLTHDDLAKEHLRHMVGGRDPLANDERLFRFEF
                     PERPGALLRFLDLMAPSWNISLFHYRNQGADYGRVLVGLQVPSGDAAAVDAFLASLGF
                     PFIEESSNPAYRLFLQTSGDA"
     gene            complement(5759..8197)
                     /locus_tag="PKY13_03230"
     CDS             complement(5759..8197)
                     /locus_tag="PKY13_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT78969.1"
                     /translation="MTEPNESTPSAAASAPPTAAEIVAGLDVATKVRLMSGEGFWWIG
                     PLTERGLDAVMVTDGPHGLRRQGDATDHLGVGGSAPATCFPPAVALGSSWDPTLVESV
                     GSAIGEEALAQGVSVVLGPGLNIKRHPGGGRCFEYLSEDPFLSGKLAAAMVRGIQSRG
                     VGTSIKHFAVNNQESYRFVVDAIVDERTLREIYLTGFEIAVKDAAPWTVMCSYNIVNG
                     TYASEHHELLTTILRDEWGFDGLVMSDWGAVSDRVAGISAGLDVEMPSSFGAFDADIT
                     AALSNGRLPVADLDRCATRVVELMQRGRRDGAEHDGAYDAHHQVARGAAAAGTVLLTN
                     SGVLPLAAAGRIAVVGAFAAEPRYQGAGSSQVNPTRVDAALDVLRTRVGDDATVTYAK
                     GYDARTGTTTDALLAEAVGAAHDADVVVVFAGLPGSYESEGFDRPSLDLPTDHVRVIE
                     AVAATSTPVVVVLNNGGVVHLPWATRIDAVLECWLGGQAGGAAAVDVLFGDAEPGGRL
                     AESIPEHVAQLASDRHFPGVPRQVQHREGLYVGYRFHDSAGVPALFPFGHGLSYTTFE
                     WSDARVTGTGTDLSVQVRVTNVGDRPGSDVVQVYVRDVESSVYRPAKELKGFAKVHLA
                     PGAHEDVTIPLDRRSFAVWDVVAHDWLVEAGDFEVLVARSSADVVAALAVHVESDDSL
                     APSVGPRHFVATDDEFVAMLGRPIPEPVPARPFTRNSTLGELEANPIGRFVGAQVVKI
                     GLKEALKEFPDPDDATLKMIRTAIREGPLRVMVQLGGGRVNFAALDSAIEAFNGHWSK
                     LASRVGRSRSRPSS"
     gene            8428..8670
                     /locus_tag="PKY13_03235"
     CDS             8428..8670
                     /locus_tag="PKY13_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOT78970.1"
                     /translation="MSAVPGSRTINVRVVTRARKDEVAGERNGRLLVRTTAPPVEGRA
                     NEAVRRILAEHFDVPARSVECISGSHSRDKVLRISE"
     gene            complement(8660..9898)
                     /locus_tag="PKY13_03240"
     CDS             complement(8660..9898)
                     /locus_tag="PKY13_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT78971.1"
                     /translation="MGSRRGTRSETDEQVAQLLRTAKDALGLSLTFLSRLDGQTQHLE
                     VVESSIPLFRDGQTQPQSTSFCQAILDGRLPPVIPDVAKLPEAMRLPAARFPRIRSYV
                     AVPVTLSDGSLYGTFCAAGFSKDRGLVERDRALLEVLSSAAATIIEPGLVEGRRRDEI
                     VQRLTPIMHAGGPTVLLQPIVSLVDGTRVGAEALSRFPADWSLPPDVVFEEASSIGAG
                     IDLEVLAFRRAADHLANVSGYVAINFSPDTFLSGRCRELLADLPADRVVLELSEHAPV
                     DDYDELAAALAPLRDRGMRLAIDDVGAGFSSLRHIVLTAPDVLKLDRSIVAGVAHDPV
                     LRTLARSLVDFAGSLGAQTVAEGIETVEDAVVLRDVGVDLGQGWLFARAGAPDAMQER
                     YAMPEATVPGASASSPAVTR"
     gene            complement(9966..10454)
                     /locus_tag="PKY13_03245"
     CDS             complement(9966..10454)
                     /locus_tag="PKY13_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOT78972.1"
                     /translation="MGDITASEAAATKRTTVERRSDREIVVTRSFDAPAQLVFEAWSN
                     PELFQRWWVPKSMGMTLYACEMDVRTGGTYRLNFGEGMDFFGRYIEVDPPSRIVWTNE
                     EDGANSSVTTVTFEEQDGGTLLVMSELFPSKEALDESGTGAAEATHETFAQLDEVLVT
                     LQ"
     gene            complement(10447..10797)
                     /locus_tag="PKY13_03250"
     CDS             complement(10447..10797)
                     /locus_tag="PKY13_03250"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT78973.1"
                     /translation="MVQYAEVDLDASFAALADGTRRGVLEQLGRAESSITDLAEKFDM
                     TLTGMRKHVGILEQAGLVTTEKVGRVRYCRIGAGRLDEVAAWIETYHHLWDARFSELD
                     KVIEQLKRKEQSDG"
     gene            complement(10861..11985)
                     /locus_tag="PKY13_03255"
     CDS             complement(10861..11985)
                     /locus_tag="PKY13_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78974.1"
                     /translation="MPTKIDLAAQLLRHGLDVVRGAYPARWTPPVRSEARWSRDRALA
                     WQDDVGWLVGCNFMPSTAGNQLEMFQPETYDPVTIDRELGWAADLGMNSIRLFLHHLL
                     PEVPGFWQRLDDVLGLADAHGIGAMLVLFDGCWNPVSHLGPQPDPRPRTHNSIWLQSP
                     GSEILSEPRRWHELRPYVDEVLGRFGDDRRVHCWDLFNEPNAPEINYIATGSADKYWL
                     ATELLDRVFDWAQAADPSQPLTAGVFVGVSGALERGDRINRLMLSRSDVITFHNYLPR
                     RRLEGCIDHLAAYGRPVLCTEWMGRPRSTPDLIDVFADRQVGCYTWGLVDGRTQTRYP
                     WSSWQKDTPPDAPWFHELLHGDGRPYDQAEVERFRRATRR"
     gene            complement(11987..12805)
                     /locus_tag="PKY13_03260"
     CDS             complement(11987..12805)
                     /locus_tag="PKY13_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78975.1"
                     /translation="MTPRTTDHQPRSWGWLGLAATVAVAGLLGTSCGSTDDEATPRSS
                     PTTKATTTLDPPASTSTMATTAAGGSLRAKRYCEVLLVQLADGNATADVYNSFPLNDC
                     PDALWTALDATAIAASEGAPIAVLNGPRFWLMDRIEKAGDVTELPKKDFGGIEMYRQA
                     SVSIGSMADAATPYLPHEVDRASVFIFDAGQTVYELTDPEGTAYVMQTWSQQKDPTLA
                     EADLADLGSKLQLPAGWAYRSRTLTEPLRIDTTTVAAAVLQDDLANSYSQITSR"
     gene            complement(12802..13440)
                     /locus_tag="PKY13_03265"
     CDS             complement(12802..13440)
                     /locus_tag="PKY13_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT78976.1"
                     /translation="MDAATRTAKRAYLRPNDRRRQLLDAASRLFDRGGLTAITMVAVA
                     AEAGASRRLVYDHFDDLASLYEAFFEDRVARYVAAVDSGFRSGEGDPLASALGAFGQL
                     LAVPSDDLRAIRLVIADTGIGELDRARLRLRSHLEQRWLPVMEQPDIDRDLAAAVLWS
                     VMDGLVGLAARAANGEIPTDTATALAAALIARLPAAVAETQTSINQQRGALR"
     gene            complement(13507..14172)
                     /locus_tag="PKY13_03270"
     CDS             complement(13507..14172)
                     /locus_tag="PKY13_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOT78977.1"
                     /translation="MSEYVEAFLLGNAAILGNVCMLPLYPGLFVLLADRAERGTSPQS
                     LRWLGVLVLAGVLSAMVAVGGLLYAVSTSFAEVLPYALPVMYGLVFVLGIAMLADRNP
                     LARVATSEVPILRNPVAGAYVYGVALGPLTLPCTGPLIISAFVLGGVRGGGALADGLV
                     YFLCFGLGFGWPLVLLPMLAAPMQRRLTGLLTSNHRLITVVSGVLLVGVAVVGWYYDI
                     RPS"
     gene            complement(14169..14735)
                     /locus_tag="PKY13_03275"
     CDS             complement(14169..14735)
                     /locus_tag="PKY13_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT78978.1"
                     /translation="MSSWVRTRHLLLVLAAFGSLLVSACGTDSTTKGDDAAASGGGAA
                     ASTWTELRLTDVAGETFAISDLAGRPVFVENFATWCSNCLRQLGDTQKAAAAAGDSAV
                     FLALSVETDLDAATVADYAEKNGFADIRFAVMTPDFLAAMSDAFGTTALNPPSTPKVL
                     VDASGAPGEMVTGFESPSEIGAKVAATT"
     gene            14822..15127
                     /locus_tag="PKY13_03280"
     CDS             14822..15127
                     /locus_tag="PKY13_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOT78979.1"
                     /translation="MSAQQPPSSAASMDLPEETLADLQKRLRRVEGQIRGIQQMLADE
                     RDCRDVVTQIAAANKALEQVGFVLVAAGLTWCLEDPSRSAEAGYELADVQKMFLKLA"
     gene            complement(15193..15609)
                     /locus_tag="PKY13_03285"
     CDS             complement(15193..15609)
                     /locus_tag="PKY13_03285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HOT78980.1"
                     /translation="MDLSPNEHDYLTTQRLGRLATLGPDGGPQARPVVFVVNDELGTI
                     DIGGNNLVASQKFRNVQRDGRVTLVVDDLETIDPWAPRGIEIRGVAEALGDGEPPQPG
                     VSALIRIRPTRVLSWGLDSNAFAPPIARNVDGPRAP"
     gene            complement(15692..16348)
                     /locus_tag="PKY13_03290"
     CDS             complement(15692..16348)
                     /locus_tag="PKY13_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT78981.1"
                     /translation="MSITVLLADDQEMIRTGLRMILSAQPGIEVVGEAADGAEAVALA
                     RQLRPDVCLFDIRMPGLDGIEATRQLAGPDVAEPQAVVVITTFDLDEYVHGALKAGAR
                     GFLLKDAGPELLVQAIKAAANGDALIAPSITARLLATFANRHDGTPPAQPVTPLTERE
                     EEVLVSVARGRTNAEIAAELFISLSTVKTHLGSLMNKLGARNRVEIAIWAHETGRIGA
                     "
     gene            complement(16345..>16588)
                     /locus_tag="PKY13_03295"
     CDS             complement(16345..>16588)
                     /locus_tag="PKY13_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT78982.1"
                     /translation="VRHARHATRVQVAVRGEPDCVRLTVHDDGEPGPFDPRTATGFGL
                     VGMAERAKLLGGTLEAGPDRNRGWTVTAVLPRGGRP"
BASE COUNT         2627 a         5645 c         5645 g         2571 t
ORIGIN      
        1 ggctcgttgg gttgcttcgg gtacaccggc ggccacggcg cgtccatgag gccggcggcc
       61 atgtcgcgcg ccgacatctc gagcagcgtc tcgatcgact gcggactcgc gttcatgggt
      121 tcccatggat cgccgttggc ggcgacgcgg cccggcacgt tggccaacgt caggtcgtcg
      181 agctcgacgg tctcgagttc gtcccaggtc agcggtgccg acacctggcc gccgacacgg
      241 gagcgcaccg accaggcgcc gaacaccgtc ttgtgcggcg cgttctggtt gaagtcgatg
      301 aagacccggt tgccccgctc ctccttccac cacgccgccg tgagctggtc ggggcgacga
      361 cgttcgagct cccgggccat cgcgaccgcg gcggcgcgca cgtcgtagct gtcccaccgc
      421 ggctcgagcc gcacgtagat gtgcaggccg cgattgccgc tcgtcttcgg gtaggcgacg
      481 atgccgatct cgtcgaggaa cgctttgacc tcgaaccctg cggcccggat gtcgtcgaac
      541 gtctgccccg gggtcgggtc gagatcgatg cgcagctcgt cggtggtctc gggcgtcgcg
      601 gcgaggtacg gccacgggtg gaagcccaga cagccgaggt tcacggccca gatgaggtgc
      661 gccatgtcgg cgaccaccaa cacgttcgag tgggtgccgt tgggcgtgct ggcgaacgtc
      721 tgctgcagcc actcgggggc gttgtcgggc acgcgcttct ggtagaacga cttcccgctg
      781 gccccatcgg ggaaccgctg gagcatggtg gcgcgacccc gaatcgtgcg catgatcggc
      841 tcggcgacgg cgaggtagta gttgacaagg tcgagtttgg tctcgccacg ttccttgaag
      901 aagaccttgc tggggctcga gatgcggacc tcacgcccgt cgacctcgat cgtcgtctct
      961 tccttcgtgg ccacgtccga acgctacgcg aggcgtcagc ggtcaggcgg ccacaccgag
     1021 cgcgtggcgg aacaggtcgg cgaactcgtc gtcgccgatg tcggcggcag ccgccggagg
     1081 gagcttgatg gtgcccttgt gggtgagcag ctcgggatgt cgcacggtga acccaccatc
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgcgcccct cgggggaggt
     1261 cggcaagttt tgtgacttcg gtcgcatagc gacgggccaa ctggggtacc tcgactcccg
     1321 ggacaccgaa gcagagcgat ccacacggag gcagagaaca tgaaccacga acgtttgacc
     1381 agccgcgcac cgacaccgac cgcgcggagg ttgcgaggcc cgctggtcgg actcgtggcg
     1441 tcgttcgccc tgatcggcgg ggcatgcgct ccgcagacgc cgccggccgg cggcggtggc
     1501 atcggcatcg acttcggacc gctcacgatc ccgctgccgc cgatcacgat ccgcccgccg
     1561 gcgacgacga tcccggtcgg gatctgcaac atcggatacc agccgccggg cgtgcagatc
     1621 atcggcgcca ccgtgacgat ccccggggtg cgggtggacc cgaacaaccc gatcatcacc
     1681 gtgccgaacg tcgtggtgaa catcccgcag ctccggttgc cgttgtccac ggtcggattg
     1741 aactgcgggc tcatcaacct gacgacccag gtcgacctgc ttctgccgtc gacggtcgtc
     1801 gtgaaggccg ccacgttgaa cattgcggca cggacgatca cgttgaccga tccgtcgttc
     1861 acggtgaacg gagcgggcct cggcatcccc ggactgggcg acctggtcgt gccgttgccg
     1921 ccgatcgtcg cggtgcccct gccgagcggg gccatcgcgt tctagaacca gcgccgtcga
     1981 gaaccaccgc gttctcgggt ccatcggtcg atgggacggc gggccttcgg gcccgccgtt
     2041 ccgcgtggtc ggagtcgatg tcgatgtcga tgtcgatgtc ggtcagcgcg acgcgagacc
     2101 gagcacgtcg gcgacgccgg tcgtgtgcag caggcgctcg acctgggcgg acgtctcggt
     2161 gacgacgatc tcgacgtcgt cgccggcacg acgcttggct tcgagcagga tgcggagccc
     2221 ctgcgagtcc atgaacgtca cgccggagag gtcgagttcg atgcgctcgg gcgcctcggc
     2281 gatggcccgg tgaaggaccg ttgccagtcg cggtgtcgtc tcgcggtcga gttcgccgct
     2341 cacggcgatc gcctggtgat ccgacgcggt cgagcagacg acctgcaatg gcgcaccccc
     2401 tccgatgaac gtcggtacgc ccttcgaggt ggtcatgcac tgcaagctac cggccccgac
     2461 cactggctcc accgtcaccg gccgcatcga cgccggtgaa gtccgaacca gggcgttcct
     2521 ggctaccatc ctcgacccat ggctcgccgt cgcaaccggc ggggtcgacg cacctggccc
     2581 cagcggctgc tcctcctcgt gaactcgctc ctcgtcgtcg cctgcctcgg cgtcgcgggc
     2641 acgttcaatc tcgtccgggc gaaggcatcc gaactcccga cgatcgacat cggcgccgaa
     2701 gcccgccaac gccccgatgc gaccgggccg cggaacatcc tgctcgtcgg caccgacagc
     2761 ggcgagggcc tcgacgagga cgacccggtc caagtcggcc gcgaaggcga gaacctcgcc
     2821 gacgtcatca tgatcctgcg cgtcgacccg tccaacgagt cggcatcatt gctgtcgatc
     2881 cctcgcgaca cctgggtacc gatcgctccg tcgtggtcca agagcaagat caactccgcg
     2941 ttcgggggca acgacggccc caacacgctc attgccacga tcaagcacaa cttcggcatc
     3001 tcgatcgaca actacgtgaa ggtcgacttc aaggggttcc gcgacatcat cgacgtgctc
     3061 gggggcatcc ccgtctacaa cgagcacccg atctctgacc ggaagaccag cctgttcctc
     3121 ccggagaccg ggtgcatcat gatcgatccg gtgcaggcgc tcgcgtacgc gcggtcccgc
     3181 cacttgcgct accagaccgg cgacacgtac gacccgaagg ccaagtggat caccgacggc
     3241 tcgagcgatc tcggccgcat cacccgtcaa caagacttca tccgacaggc ggcacaacgc
     3301 gccatcgacg agggcatcaa gaacccgacg acggcgtacg gcctcatcaa cgcggcgctc
     3361 ggatcggtcg agaaggacga cgatctgagt gtcggcgaga tccaggacct catcgtgacc
     3421 ttccgggact tctcggtgca ggaactgacc tccgagcaga tcccgaccgt ctccggcggg
     3481 aatccgaaga tctcctacca gttggtcgtc tgggatcagg cggagggact tctcgacatc
     3541 taccgcggga tccgcgaacc ggggcaggtg cagccgagcg acgtgatcgt cggccttccg
     3601 gcgtcggtgc ccacgtcggc cgcgctcagc gagcagctcg acgcgatcgg tttcgacgcc
     3661 ggcaccgagg actcgtcggt gaagtcgagc ggcaagcgac cgggcaccgt gatccggtac
     3721 ggcctgcgcg gcatcgaggc ggcccgagtg ctcgcctcgt ggctcgacgg cgaggcgacc
     3781 tacgagttcg acgccgatct cccgggtcgc cgcctcgaac tcctcccggg caccgctccc
     3841 ctcgcgttgc tcgagacggc acgaccgctc gaccaggtgc cggtgcccgt catcaacgag
     3901 cggcgcaccg gcaaggggac caccacgacg agctcgacca cgtcgacgac cgtgccgacg
     3961 acgagcacca cggcgacgtc cggctcgggc gcgccatcga ccacgaccac gaccacgacg
     4021 acgaccgctc ccgagtcgcc gacgaccacg gcgatcggct tcctgccgct cgacgccgaa
     4081 cgcgccgcga gctgctgaac ggcgctaggc gtcgccgctc gtctggagga agaggcggta
     4141 ggccgggttg ctgctctcct cgatgaacgg gaacccgagt gacgccagga acgcatcgac
     4201 cgccgctgcg tcaccgctcg gaacttgcag gccgaccagc acccgcccgt agtcggcgcc
     4261 ctggttccgg tagtggaaga ggctgatgtt ccagctcggt gccatgaggt cgaggaagcg
     4321 gagcagcgct ccgggacgct cggggaactc gaagcggaac agtcgctcgt cgttggccag
     4381 cggatcacga ccgccgacca tgtgccgcag gtgttccttg gccaggtcgt cgtgggtcag
     4441 gtcgatcgtg gcgaagcccg cggcgcggaa ccgatcggcg atcgtcgtgg actcaccacg
     4501 aaccgacgtg gtgagtccca cgaacacgtg ggcgtcggag cggccgctca agcggtagtt
     4561 gaactcggtc acgttgcggg gcccgccggg gagcgaaccg accagctcgg cgaaccggcg
     4621 gaagctcccg gggacctcgg ggatcgtgac ggcgagcagc acctctcgct cctcgccgac
     4681 ctcggctcga tcggcgacga aacggaggcg gtcgaagttc atgttggcgc cgcacaggat
     4741 cgtcgcatag gtctcgccga gcgtgccgtg gtcggcgacg taggccttga ggccggcgag
     4801 cccgagcgct ccggccggct cgacgatgct gcgcgtgtcg aggaacacgt ccttgatcgc
     4861 ggcgcacacg gcgtcggtgt cgacgcggat caggtcgtcg acgaggtcgg tgacgatgcg
     4921 gaacgtctcc tcgcccacca tccggaccgc cgtgccgtcg gagaacagtc cgacgttctc
     4981 gagctcgacg cggtggccgg cgtcgacgga acggatcatc gcgtcggagt ccgacgtctg
     5041 cacaccgatg accttgatct cgggccggac cgccttgacg tagttggcga tccccgagat
     5101 cagacctccg cctcccaccg cgacgaagac cgcgtcgagg cggccttggt gctggcgcaa
     5161 cagctccatg gcgacggtgc cctgccccgc gatcacgtcg ggatcgtcga acgggtggac
     5221 gaacgtgagc ccgcgctcgc gctgatgcac cagcgcgacc tcgtgggcgt cggagtagct
     5281 ctctcctgcc agcaccacct cggcaccgag cgctcgcacg gcgtcgacct tgagttccgg
     5341 cgtcgtgacc ggcatgacga cgacggccct cgtgcccaac tcccgcgcac tcagcgccac
     5401 gccctgggcg tggttaccgg ccgacgcgca gatgacccca cggtccagcg cggcggcgtc
     5461 gaggttcgcc atcttgttgt aggccccgcg cagcttgaag ctgaagacgg gctgttggtc
     5521 ctcgcgcttg agcagcaccc ggttgccgag gcgctcactg agccgtggcg ctgcgtcgag
     5581 cgacgactcc acggcccggt cgccgatctg cgccgtgagg atccgcttga ggtagtcggt
     5641 cggcgtcagc ccggttgcag gctcgtcagt ggaaggctca tcggtggaag tcatcgcgtc
     5701 gcgatcgtag gggcgaccga tgaccgatcc tccacagttg aactgtggag gcgaatcgtc
     5761 acgacgaggg tcgtgaccgg gagcggccga cgcgggaggc gagcttcgac cagtggccgt
     5821 tgaacgcctc gatcgccgag tcgagcgcgg cgaagttgac ccgcccgcct cccagctgga
     5881 ccatcacccg gaggggcccc tcgcggatcg cggtgcggat catcttgagc gtggcgtcgt
     5941 ccggatcggg gaactctttc agcgcttcct tcaggccgat cttgacgacc tgcgcgccga
     6001 cgaaccgccc gatcgggttg gcctcgagtt ctccgagtgt cgagttccgg gtgaacggcc
     6061 gcgccggcac cggttcgggg atcggccgac cgagcatggc gacgaactcg tcgtcggtcg
     6121 ccacgaagtg ccggggcccg accgacgggg cgagtgagtc gtccgactcg acgtgcaccg
     6181 ccagcgcggc gacgacatcg gcggacgagc gcgcgacgag cacctcgaag tcgccggcct
     6241 cgaccaacca gtcgtgggcg acgacgtccc agacggcgaa cgatcgccgg tccagcggaa
     6301 tggtcacgtc ctcgtgcgcg cccggagcga ggtgcacctt ggcgaatccc ttgagctcct
     6361 tggccgggcg gtagaccgac gactccacgt cgcgcacgta gacctgcacc acgtccgaac
     6421 cgggccggtc accgacgttg gtgacccgca cctgcacgga gaggtcggtt ccggtgccgg
     6481 tcacccgggc gtcggaccat tcgaacgtcg tgtaggacag gccgtggccg aacgggaaca
     6541 gtgcgggtac tccggcgctg tcgtggaacc ggtacccgac gtacagaccc tcgcggtgct
     6601 gcacctggcg tggcacaccc gggaagtggc ggtccgatgc gagctgggcc acatgctcgg
     6661 ggatgctctc ggcgagccga ccaccgggtt cggcgtcgcc gaacaggacg tccactgctg
     6721 ctgccccgcc ggcctggcca ccgagccagc actcgaggac tgcgtcgatt cgcgtggccc
     6781 acgggaggtg cacgactccg ccgttgttga gcacgacgac gaccggggtc gacgtcgccg
     6841 cgaccgcctc gatgacgcga acgtgatcgg tgggcaggtc gagtgacggt cggtcgaatc
     6901 cctccgactc gtatgagccc gggagtccgg cgaacaccac cacgacgtcg gcatcgtggg
     6961 cggcgccgac cgcctcggcc aacagagcat cggtcgtggt cccggtccgg gcgtcgtagc
     7021 ccttcgcgta ggtgacggtg gcgtcgtcgc cgactcgcgt ccgcagcacg tcgagggcgg
     7081 cgtcgacgcg ggtcgggttg acctgggagc tcccggcccc ctggtacctc ggctctgcgg
     7141 caaaggcccc gaccacggcg atgcgtccag cagcagcgag cggcagcacc ccgctgttgg
     7201 tcaggagcac cgtgccggca gcggcagcgc cgcgggcgac ctgatggtgg gcgtcgtacg
     7261 cgccgtcatg ctcggcaccg tcgcgccgac cgcgctgcat cagttcgacg acccgggtcg
     7321 cacagcggtc gaggtcggcg accggaagcc gcccgttgga cagcgcggcg gtgatgtcgg
     7381 cgtcgaatgc accgaagctc gacggcatct cgacgtcgag gcccgcgctg atgccggcga
     7441 cgcggtcgct caccgcgccc cagtcgctca tcaccagtcc gtcgaaaccc cactcgtcgc
     7501 ggaggatggt cgtgagcagc tcgtggtgct cggaggcgta ggtgccgttg acgatgttgt
     7561 agctgcacat gaccgtccac ggcgccgcgt ccttgaccgc gatctcgaaa ccggtgaggt
     7621 agatctcgcg cagcgtgcgc tcgtcgacga tggcgtcgac gacgaatcgg tacgactcct
     7681 ggttgttgac ggcgaagtgc ttgatgctcg tgccgacgcc gcgggactgg atgccccgca
     7741 ccatggcggc ggcgagcttg ccgctgagaa acgggtcctc cgacaggtac tcgaagcagc
     7801 gcccgccacc gggatgccgt ttgatgttga ggcccgggcc gagcacgacc gacacgcctt
     7861 gcgcgagcgc ctcctcgcca atggcgctgc cgaccgattc gacgagcgtc gggtcccagc
     7921 tcgagccgag cgcaaccgcc ggcgggaagc aggtcgccgg agccgaaccg ccgacaccga
     7981 ggtgatcggt ggcgtcgccc tgccggcgga gcccgtgggg accgtcggtg accatgacgg
     8041 cgtcgagtcc acgctcggtc agcggaccga tccaccagaa gccctctccc gacatcagtc
     8101 ggaccttcgt ggcgacgtcg aggccggcga cgatctcggc agcagtcggg ggcgcagacg
     8161 cggccgcgct cggggtcgat tcgttgggtt cggtcacgtc ggggaaccta gttcccgatc
     8221 gtccggccgt ccgttctacg gtgcgcccgt gatcttccgt cgtgaaccca gcgctccgaa
     8281 cgcgatgttc gcggtcgtgg acggacccgt ccaccgccct gccgctcccc cccggtcgag
     8341 ctctcgtttc gcagccactc ccgccagcac catcggctcg gtgatcggtg gggagctcgc
     8401 cccggggtgc ggcggtagcg ttgcctcgtg tcggcggttc cgggatcgag aacgatcaac
     8461 gttcgtgtcg tgactcgcgc caggaaggac gaggttgccg gcgaacgcaa cggtcgcctg
     8521 ctcgttcgga ccactgcgcc gccggtcgag ggtcgggcca acgaagccgt ccgacgaatc
     8581 ctcgccgagc acttcgatgt tcctgcgcgt tcagtggagt gcatctcggg cagccactcc
     8641 cgcgacaagg tgctccgaat cagcgagtga ccgcaggcga cgaggcggac gcgcccggaa
     8701 ccgtggcctc cggcatcgca tagcgctcct gcatcgcgtc gggtgctccg gcgcgggcga
     8761 acagccaacc ctgaccgagg tcgacaccga cgtcgcgcag aacgacggca tcctcgacgg
     8821 tctcgatccc ctcggcgacc gtctgggcac cgagcgaccc cgcgaagtcg accaaggacc
     8881 gtgcgagcgt tcgcagcacc gggtcgtggg cgacgccggc aacgatgctc cggtcgagct
     8941 tgaggacgtc cggtgcggtg aggacgatgt gacggagcga cgagaatccg gcgccgacgt
     9001 cgtcgatcgc cagtcgcatg ccccggtcac ggaggggggc cagcgccgcg gcgagctcgt
     9061 cgtagtcgtc gacgggtgcg tgctcggaga gctccaggac gacccgatcg gcaggcagat
     9121 ccgcgagcag ttcacgacat cgaccgctga ggaacgtgtc cggcgagaag ttgatggcga
     9181 cgtacccgct gacgttcgcc aggtggtccg ctgctcgacg gaacgccagc acctccaggt
     9241 cgataccggc cccgatcgac gacgcctctt cgaagacgac gtcgggtggg agggaccagt
     9301 cggcgggaaa ccggctgaga gcttctgcgc cgacgcgggt tccgtcgacc aacgacacga
     9361 tgggctgcag caggacggtc ggccccccgg cgtgcatgat cggagtcaat cgctggacga
     9421 tctcgtcgcg gcgacgaccc tcgacgagcc cgggctcgat gatcgtcgct gcagcgctcg
     9481 agagcacttc gagcagtgcc cggtcgcgct cgaccaggcc ccggtccttg ctgaacccgg
     9541 cggcgcagaa ggtgccgtac aacgagccgt cactcagggt gacgggcacg gcgacgtagc
     9601 tccggatccg ggggaaccgt gctgcgggca gccgcattgc ctccggcaac ttggcgacgt
     9661 cggggatgac gggcggcagt cgcccgtcga ggatcgcttg acagaacgac gtgctctgcg
     9721 gctgggtctg gccgtcgcgg aacaagggga tcgacgactc gacgacctcg aggtgctggg
     9781 tctgcccgtc gaggcggctg aggaacgtga ggctcagccc cagcgcgtcc ttcgcggttc
     9841 gaaggagttg cgctacctgc tcatcggtct cggatcgcgt tccacgccgg ctgcccatcg
     9901 ctccctatcg gccatgccga cccgctcctg aggagggctc gggcggcagc aggccgcccg
     9961 agccctcact gcagcgtgac cagcacctcg tccagctgcg caaacgtctc gtgcgtcgct
    10021 tccgccgcac cggtccccga ttcgtcgagc gcttccttcg agggaaacag ctcgctcatc
    10081 acgagcagcg tcccgccatc ctgctcttcg aacgtcacgg tggtgaccga gctgtttgct
    10141 ccgtcctcct cgttcgtcca cacgatccgc gacggcggat cgacctcgat gtagcggccg
    10201 aagaagtcca tgccttcgcc gaagttgagc cggtacgtgc ccccggtgcg cacgtccatc
    10261 tcgcaggcgt acagcgtcat gcccatcgac ttcggcaccc accacctctg gaacagctcg
    10321 ggattcgacc atgcctcgaa caccagttgc gccggagcgt cgaacgagcg ggtcacgacg
    10381 atctcgcggt cagacctccg ttccactgtc gtgcgtttcg tcgctgcggc ctcacttgcc
    10441 gtgatgtcac ccatctgact gctccttccg tttcaactgt tcgatgacct tgtccagctc
    10501 ggagaaccgc gcgtcccaca ggtggtggta cgtctcgatc cacgccgcga cctcgtcgag
    10561 gcggcccgcc ccgatccggc agtagcggac ccgtccgacc ttctcggtgg tgacgagacc
    10621 tgcctgctcg agaatgccga cgtgtttcct catcccggtg agggtcatgt cgaacttctc
    10681 ggcgaggtcg gtgatcgagg actccgcgcg tcccagctgt tccagcacgc cgcgacgggt
    10741 gccgtccgcc aacgctgcga acgaggcatc gagatcgacc tcggcatact gaaccatacg
    10801 gttcagtatc tctcgcgccg ctcggcgatg caagcgcttc gtcgaacgat cggcggcggc
    10861 tcagcgacgg gtcgctcgtc ggaaccgctc gacctccgcc tggtcgtacg gcctgccgtc
    10921 gccgtggagg agctcgtgga accagggggc gtcgggcggg gtgtccttct gccagctgct
    10981 ccacgggtac cgcgtctgcg tccgcccatc gaccaacccc cacgtgtagc agccgacctg
    11041 acgatcggcg aacacgtcga tcaggtcggg agtggatcga ggtcgaccca tccactcggt
    11101 gcacaacacc ggtcgtccgt aggccgccag gtggtcgatg cacccctcga gccgccgacg
    11161 cgggaggtag ttgtgaaagg tgatcacgtc cgatcgggac aacatgagcc ggttgattcg
    11221 atcgccccgc tccaacgcgc ccgacacccc gacaaacacc ccggccgtga ggggctggga
    11281 cgggtccgcc gcctgcgccc agtcgaacac ccggtccagg agctcggtcg cgagccagta
    11341 cttgtcggcc gatccggtag cgatgtagtt gatctcgggt gcgttgggct cgttgaacag
    11401 gtcccaacag tgcacccgac ggtcgtcgcc gaaccttccg agcacctcgt cgacataggg
    11461 gcgcagctcg tgccaccggc gcggctccga caggatctcc gaccccggcg actgcaacca
    11521 gatcgagttg tgcgtgcgcg gccgcgggtc gggttggggg cccaggtgcg acaccgggtt
    11581 ccagcacccg tcgaacaaca ccagcatggc cccgatgccg tgcgcatcgg cgaggccgag
    11641 cacgtcgtcc aaccgttgcc agaagcccgg cacctcgggc agcaggtggt ggaggaacag
    11701 ccggatgctg ttcatgccga ggtcggccgc ccatcccagc tcccggtcga tcgtcacggg
    11761 gtcgtacgtc tcgggctgga acatctccaa ctggttgccc gctgtcgacg gcatgaagtt
    11821 gcacccgacc aaccaaccga cgtcgtcctg ccatgcgagg gcgcggtctc gactccatcg
    11881 cgcctcggag cggaccggcg gcgtccaccg cgccgggtac gcgccgcgca ccacgtcgag
    11941 tccgtggcgc agcagctgcg ccgcgagatc gatcttcgtc ggcacgtcag cgggaggtga
    12001 tctgcgagta actgttggcg agatcgtcct ggaggacggc ggcggcgacc gtggtcgtat
    12061 cgatccgaag cggctcggtg agcgttcgtg agcgataggc ccagccggcg gggagttgca
    12121 gcttcgagcc cagatcggcc aggtcggcct cggcgagggt gggatccttc tgctggctcc
    12181 aagtctgcat cacgtacgcc gtcccctcgg ggtcggtcag ttcgtagacg gtctgaccgg
    12241 cgtcgaagat gaacacgctg gcgcggtcga cctcgtgggg cagatacggg gtcgcggcgt
    12301 cggccatcga gccgatcgac acggatgcct ggcggtacat ctcgattcct ccgaagtcct
    12361 tcttcgggag ttcggtgaca tcacccgcct tctcgattcg gtccatgagc cagaaccgcg
    12421 gcccgttcag caccgcgatc ggcgcgccct ccgacgccgc gatcgccgtg gcgtcgagtg
    12481 cggtccacag cgcgtcggga cagtcgttga gcgggaaact gttgtacacg tcggccgttg
    12541 cgttcccgtc ggccaactgg acgagcagga cctcgcagta tcgcttcgcg cggagactcc
    12601 ctcctgccgc ggtggtcgcc atcgtcgacg tgctcgccgg cgggtcgagg gtcgtggtcg
    12661 ccttcgtggt cggcgaggac cggggggttg cttcgtcgtc ggtcgaaccg caggaggtgc
    12721 cgagcagccc cgcgaccgcc acggtcgccg cgaggccgag ccacccccag gaacgcggtt
    12781 gatggtcagt ggttcgcggt gtcatcggag agctcctcgt tgttggttga tcgatgtctg
    12841 tgtctccgcg accgcggcgg ggagccgggc gatgagcgct gcggcgagtg cggttgcggt
    12901 gtcggtgggg atctcgccgt tggcagcgcg ggcggcgaga cccacgagac cgtccatcac
    12961 ggaccacagc accgcggcgg cgaggtcacg gtcgatgtcg ggttgctcca tcaccgggag
    13021 ccaccgctgc tcgaggtgtg agcggagccg aaggcgggct cggtcgagtt cgccgatgcc
    13081 ggtgtccgcg atcaccagcc gaatggcccg aaggtcgtcg gacgggacgg cgagcaactg
    13141 accgaaggca ccgagggccg acgccaacgg gtcgccctct ccggaacgga aaccgctgtc
    13201 gaccgctgcc acgtagcggg cgacccggtc ctcgaagaac gcctcgtaca gcgacgcgag
    13261 gtcgtcgaag tggtcgtaca cgagccggcg gctggccccc gcctccgctg cgacggcgac
    13321 catcgtgatc gccgtgaggc cgccgcggtc gaacagtcgt gatgccgcat cgagcaactg
    13381 gcggcgtcgg tcgttcggcc ggaggtacgc ccgcttggcg gttcgtgttg ccgcgtccac
    13441 ccgtcgccac cctcctcgcc ggaactgtac atcatgtgta gttctcggca cgcagacggt
    13501 acccggtcag ctcggacgaa tgtcgtagta ccagcccacc accgcgacgc cgacgagcag
    13561 cacacccgac acgacggtga tcaaacggtg gttgctggtg agcagccccg tgagccggcg
    13621 ctgcattggc gccgccagca tcgggagcag gacgagcggc cagccgaacc ccaggccgaa
    13681 gcacaggaag tacacgaggc cgtcggccag cgcaccccca cccctcaccc cgccgagcac
    13741 gaaggccgag atgatcagcg ggccggtaca cggcaaggtc agtggcccga gcgccacgcc
    13801 gtacacgtag gcaccggcga ccggattgcg gaggatcggc acctcgctgg tcgcgactcg
    13861 ggccaacggg ttccggtccg ccaacatggc gatcccgagc acgaacacca ggccgtacat
    13921 gaccggcaac gcgtacggga gcacctccgc gaacgaggtg ctcacggcgt acaacagtcc
    13981 gcccacggcg accatggcac tcaggactcc ggcgagcacg agcactccca gccagcgaag
    14041 cgactgcggc gatgtgccac gctcggcccg gtcggccaac aacacgaaca gcccggggta
    14101 cagcggcagc atgcagacgt tgccgaggat cgcagcgtta ccgaggagga acgcctcgac
    14161 gtactcgctc acgtcgtggc agcgaccttc gcgccgatct ccgatgggct ctcgaacccg
    14221 gtcaccatct cgccgggcgc tcccgacgcg tcgaccagca ccttcggggt gctcggcggg
    14281 ttcagcgccg tcgtgccgaa cgcgtcgctc atcgccgcga ggaagtccgg cgtcatcacg
    14341 gcgaaacgga tgtcggcgaa gccgttcttc tcggcgtagt cggccacggt cgctgcatcg
    14401 agatcggtct cgacgctgag ggccaggaac accgccgagt cgccggcggc cgctgccgcc
    14461 ttctgcgtgt caccgagctg gcggaggcag ttcgaacacc acgtcgcgaa gttctcgacg
    14521 aacaccggtc ggccggccag gtcggagatc gcaaacgtct cgccggcgac gtcggtgagt
    14581 cggagttcgg tccacgtcga cgcagcggcg ccgccgccgg atgccgccgc gtcgtccccc
    14641 ttcgtcgtcg agtcggtccc gcaggccgat accaagaggc tgccgaacgc agcgaggacg
    14701 aggaggaggt gtcgggttcg gacccaggac gacatacgag cggcctttcg acggtgaagg
    14761 atcgggtgca ggtcgcgctc gacagcaccg gtacccccga gggtacccct acactccacc
    14821 catgagcgct cagcagcccc cctcctccgc cgcatccatg gaccttcccg aggagaccct
    14881 cgccgacctg cagaagcgcc tgcgccgggt cgaagggcag attcgcggga tccagcagat
    14941 gctcgccgac gaacgggact gccgggacgt cgtcacgcag atcgctgcgg ccaacaaggc
    15001 cttggagcag gtcggattcg tcctcgtcgc cgccggactg acgtggtgcc tcgaagaccc
    15061 gtcgcgctcg gccgaggcgg gatacgagct cgccgacgtc cagaagatgt tcctcaagct
    15121 cgcctgaacg actcggggcg acacgtccga ccaaggagaa cgtcatgaac gaaggaacca
    15181 ccgatcggcg tgtcacggcg ccctcggccc gtcgacgttg cgagcgatcg gtggggcgaa
    15241 cgcgttgctg tcgagacccc aggagagcac ccgggtcgga cggatcctga tgagggcgga
    15301 gacacccggt tgcggtggtt cgccgtcacc cagcgcctcg gccacaccgc ggatctcgat
    15361 gccgcgcggc gcccacgggt cgatggtctc gaggtcgtcg acgacgaggg tcacgcgacc
    15421 gtctcgctgg acgttgcgga acttctggct ggcgacgagg ttgttgccac cgatgtcgat
    15481 cgtgccgagt tcgtcgttga cgacgaagac cacgggtcgg gcctgagggc caccgtccgg
    15541 cccgagggtg gcaagccggc cgagccgctg cgtggtgagg tagtcgtgct cgttcggtga
    15601 gagatccatc gtgttgccct tcgagatcgg cgagtgggag tcaccaaact acggactcag
    15661 tcatcctccg gccgtgggcg aatcgcccgc ctcaggcgcc gatgcgtccg gtttcgtgag
    15721 cccagatggc gatctcgacg cggttccggg cgccgagctt attcatcaag ctgccgagat
    15781 gggtcttgac cgtgctcaga ctgatgaaga gttcggcggc gatctcggcg ttggtccgac
    15841 cccgagcgac ggacacgagc acctcttcct cccgctcggt cagcggggtg acgggctgcg
    15901 ccggaggtgt gccgtcgtgt cggttggcga acgtggccag caaccgggca gtgatgctgg
    15961 gggcgatgag cgcatcacca ttggcggcag ccttgatggc ctgcaccagc aactcggggc
    16021 ccgcgtcctt cagcaggaat cccctcgctc ccgccttgag ggcgccgtgc acgtactcgt
    16081 cgagatcgaa cgtggtgatg acgaccacgg cctgtggctc ggcgacgtcg gggccggcga
    16141 gctggcgggt tgcttcgatc ccgtcgaggc cgggcatgcg gatgtcgaag aggcagacgt
    16201 ccggtcggag ttgcctggcc agcgcgacgg cttcggcgcc gtcggcggcc tcgccgacga
    16261 cctcgatgcc cggctgggcg ctgaggatca tccggagccc ggtccggatc atctcctggt
    16321 catccgcgag caggacggtg atgctcatgg ccggccaccc cgtgggagca cagcggtgac
    16381 ggtccatccc cggttgcggt cggggcccgc ctcgagggtg ccgccgagca gcttcgcccg
    16441 ttcggccatc ccgaccagcc cgaaccccgt tgcggtcctg ggatcgaatg gccccggctc
    16501 gccgtcatcg tgcaccgtca ggcggacgca gtcgggctcg ccccgtaccg ccacctgcac
    16561 gcgggtggcg tggcgggcgt ggcgcacg
//
LOCUS       DAOSOA010000026        16506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3540_length_16506_cov_6.835086, whole genome shotgun sequence.
ACCESSION   DAOSOA010000026 DAOSOA010000000
VERSION     DAOSOA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16506
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3540_length_16506_cov_6.835086"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..650)
                     /locus_tag="PKY13_03300"
     CDS             complement(126..650)
                     /locus_tag="PKY13_03300"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOT78983.1"
                     /translation="MAPTSTTLHRRIYASPLGELTLTASEQGLRSLTWPEEAGGRAPA
                     DGPPIGDEPPDVAAVFDHVVAQLDEYFAGERTEFDVPLDPVGTEFQLRAWAALRRIPY
                     GETRSYGEQARTIGSPAAVRAVGAANGRNPISIIVPCHRVVGSDGSLTGFGGGLDAKR
                     YLLDLETRTQTLFG"
     gene            complement(650..2146)
                     /locus_tag="PKY13_03305"
     CDS             complement(650..2146)
                     /locus_tag="PKY13_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HOT78984.1"
                     /translation="MFDDHDRCYRIVQGRDPRFDGCFVTAVRTTGIYCRPSCPARTPK
                     PANVEFFPTAAAAQLAGYRACKRCRPDASPGSPEWNGRSDVVARAMRLIADGVVDREG
                     VPALARRLGYSDRHLNRLLLAELGAGPLALARSQRAQNARVLIETTSIPMVDVAFAAG
                     FASLRQFNDTVRQVFAASPTELRVAAARRRPAERSAATGAANTLSLRLAHRRPLAAPA
                     LWEFLRARCIAGTESFDLSGSVEVYRRTLDLPLGHGVVALAPGDGHVDAVLRLADVRD
                     LTAAVARCRRLLDLDADPEAIDARLSLDSALAPLVAARPGLRSPGAVDGFEMAVRAVL
                     GQQVSVAASIRSAGRFVAELGEELSFEDTGLDRLFPTPDALAALDPESLPLPRQRAAA
                     LVQLGDAVASGRLRLDPGADRSEARQVLLGIPGIGPWTADYVAMRALGDPDVMAGGDL
                     VVRQRAARLGIGPSELSARSLEWAPWRTTVVHHLWAADDSTTTEKEAA"
     gene            complement(2199..2666)
                     /locus_tag="PKY13_03310"
     CDS             complement(2199..2666)
                     /locus_tag="PKY13_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT78985.1"
                     /translation="MTEAQAITHHAVVGFDGSENSAAALQWAFDHVQRLGGGTIKVVM
                     AWNYAPTAVGGYGLAGGGLPPADSMQEATEQGLAESLTTITPPEGITLESVVREGPAA
                     KILVEESEGAEILVVGKRGHGGFLGLLIGSITNQVANHATCPVVIVPGPANVE"
     gene            complement(2694..3815)
                     /locus_tag="PKY13_03315"
     CDS             complement(2694..3815)
                     /locus_tag="PKY13_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOT78986.1"
                     /translation="MSFRSLTVRLLAPGALLVSAACVGATDATVATGAISPSSTVTTT
                     TIAVPACVAALSVEQKAGQLLMVLVPTPAAAGDLVAAGRVAGYALVGAQTGDVTAEVA
                     AVAARSKLPVFASGDEEGGTVQRFRDMLGALPSAAALARDNGPEKAAQIFGEYGAKLR
                     ALGLNMNFGPSLDVGGGSGLGTRSFGDDVDQVSSYGIAVIGAVKAAGLIPVAKHWPGI
                     GGGEADPHDSASPVDELDALKQRDLIPFDRAIAAGLDALMVSHAIIPGLTDGMPASLS
                     RAAITDELRGREKYDGLVITDSLGMGAIAANYDNAGAAVLSIMAGADIALVSLAESVP
                     TAQLNVIAAINDGRIPMAQVDRSVARVLTAKGITGDCPA"
     gene            complement(3867..4286)
                     /locus_tag="PKY13_03320"
     CDS             complement(3867..4286)
                     /locus_tag="PKY13_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78987.1"
                     /translation="MRVEIGPVGVDTAVAWIAYGRRCVDHLAAANRPSRANVLDRFHS
                     LLDEFADAASAGNPFHWVTDVSPEEVEFVMKGLFEIGLVVEAEHEAGRLPLRPPAADE
                     FHHMVVKQVLAEIEAEGPAFAQFVEGLRGEWGVAGSA"
     gene            complement(4351..6069)
                     /locus_tag="PKY13_03325"
     CDS             complement(4351..6069)
                     /locus_tag="PKY13_03325"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOT78988.1"
                     /translation="MIAVRGLMAVVALALTLPATGATTLAPEVCAPDVGVVTLTGTVT
                     PDLAKTYRVEPFEVATGTTRVEVGYSWTPSEGTTLDLGLWDAAGYRSPEGFRGWGGSR
                     EGRLDQGMSRAWVQADSAERAFTPGTVEAGTWYVELGFAEVAPAGATWTVELRCTDDP
                     VGPEIDADPVDASHVADPDPGWYRGDFHMHGFHSNPEAPDNEGFVQYARDANLDFLPV
                     TEYVVNRHWNEWGEMQRANPDLLIWPGREVITYFGHAIVLGETPSTVEYRHGFEDVTL
                     GSIQDDVLADGALFGIAHPTVFPERDFGSTCRGCEFQLSDTIDPDRVDTVEVVTDAVA
                     YGTAPNPFVATAIDFWTNLLVSGHRVTAVSGSDDKLGPDLGSVATVVYADELSRPALQ
                     RAVRAGHAYVQTRGASGSPSLELTATTPEGGEVMMGDTVVGDTSTMTVRVTDGAGQRL
                     RVLRNGVEAAVVDVTSSPFVHSFTADRTDDEGPLGTFWGVQTFDDLSLTTISNPIFLA
                     DAAPTVAPAPSTTAAPAPAPAPTAIADRPDGSGGSSWRGPWTAAGALVVVVLLLVLAR
                     RRVRGR"
     gene            complement(6066..7028)
                     /locus_tag="PKY13_03330"
     CDS             complement(6066..7028)
                     /locus_tag="PKY13_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOT78989.1"
                     /translation="MTVERSVAGRLRRLTEELAEEGFAIDVTDPVGSVLLAELDYALR
                     PAVHERRIPSFGAIVEPGADPSGWDAATDLSVTHRHLEGFPARDARRFADGLSSWLVR
                     RGDRDLELAVFDRPAGSERDLVVMAQVMDATIVQRHPSGLVRIVGDVGVLRWDGLSWH
                     RESPVSDWIDAMTACRQHGDRDVLGRLLEFAVHDLGARGIGALLIYQPLAPDLPTWEV
                     RLPEPPPLDINRPADLAPLRHVLGQVDGAAVFDDVGTLRQLGVRLVPSPEAEADVDAV
                     RGTRHTSARRYSFDDPTATLIVISEDGPVMVMRNGEILGRSELP"
     gene            7275..8213
                     /locus_tag="PKY13_03335"
     CDS             7275..8213
                     /locus_tag="PKY13_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT78990.1"
                     /translation="MAKQRPERDEEDLVRLYLSDIGQYPLLTKDGEVTLAKAIEAGVA
                     ARTEFEANERTLTAARKRELRRTIRAGEEAELAFVQSNLRLVVSIAKKYQASNLPLLD
                     LIQEGNLGLIHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDVLS
                     RLQKARFRLETSLGRPATLAELSVELDMPIAKVTEAMRYAADTLSLSEPLRADGDAEL
                     GDMIEDRGAESPFESAAVALLPDQINRLLFPLDERERQILKLRFGLDRGEPRTLEEVG
                     EHFNLTRERIRQIEARAMSKLRHPSSDTGAKDLLAV"
     gene            8352..9674
                     /locus_tag="PKY13_03340"
     CDS             8352..9674
                     /locus_tag="PKY13_03340"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT78991.1"
                     /translation="MFVFLLQVRDTLANGRVYLFTIFVTLVWVVWLRRVQLSRRYRPY
                     TEDVSLTTSVIIPVVDEPEELFDEVLRRIVAQGPDEVIVVINGPRNEPLERICERFDR
                     VIWRWTEVPGKRNALRVGVDAMLGDIAVLVDSDTIWTTGTLRELLKPFADERVGGVTT
                     RQHILAQERHVLTRWADWMESVRNEYSMPAMSVLGTVGCLPGRTIAFRRQILVDAMPA
                     FLSERFLGIFLEVSDDRTLTNEALKQGYRTVYQATSEVYTDAPTTWKKMAKQQLRWAR
                     GSQYNTARMLPWMLRNARTLAFFYVCDIVLPFLLVCTITGWVFRASRVDSPDIYGAFV
                     AHYGRWVALGLMITLSVVMSTASSAIRQARHLKRRPDDLLRLPAFILISTFFLMPIRL
                     FGFTRMGHNSGWGTRSGGYSGERNRNPLAAIPYLIAVAIVVAGTVLPS"
     gene            9703..11244
                     /locus_tag="PKY13_03345"
     CDS             9703..11244
                     /locus_tag="PKY13_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT78992.1"
                     /translation="MPRHLRSTPSTPDTEPTAVPAAGSELPIHLVATPVARGLRRLGV
                     GTVPFTIVAMVTLAAMSAFVVMSPTSIPGLGAPDELASVDLGVGIDGAPNRSLDPDVT
                     TTISVAATTTASTEPSTTTATPQATSRPTTSTAPPSSAKPASTPTGELASTPVSVRPV
                     RCEDFTLQPEAQLSFEADPIGRAGLDGDADGIACEQLPGRTRNALPALSARRIPTVEQ
                     VRRPATRLFGLHTPQAPFATSEIEAFSVAAEKTPNSLLFFQNFSQEFPLSAVTTSWSR
                     GMLPMVTFEPIIQDSPSGQPMLADIADGVWDEYFTRWATAAKAEGHPIAFRFAQEMNG
                     NWYSWSDGRFGNAPGDFVRAWRHVHQIFDDIGADNVLWVWSVNRVDNLPDKTIARVYP
                     GDEWVDWVGASGYLRNVPDGVTPTFEYTFGQTLDAIEAVAPTKLVMLTEVGAGTTESN
                     RVSWLSSFFQGLLDRPEIIGFNWFNDFKDGGDWRIQYSTATSAAFAAGVADQRYGDLA
                     PLLPA"
     gene            11400..12029
                     /locus_tag="PKY13_03350"
     CDS             11400..12029
                     /locus_tag="PKY13_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT78993.1"
                     /translation="MKSFARNRKNKRTDGIDPDTAEGLIDVDELDIAPLGVAPGTRSI
                     PGSDDPNADEAADFAERLGDLDRFIGGGTAPATGPTTSVSWTGGAPARNLPAAPIPAV
                     APISHPRPASFDRAAELEPQSGAVERPVADPEAEAQEFVDRLGDLDRFIGGNGGAPTP
                     AAATTPEIPVADPDAEAQDFLDRLGDLDRFIGGNGSGGNDADEQRPHRD"
     gene            12077..14389
                     /locus_tag="PKY13_03355"
     CDS             12077..14389
                     /locus_tag="PKY13_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOT78994.1"
                     /translation="MTNPASGAPIDALSGPARPSSLVVVGAAAGMGRWLTDHLLLDRH
                     WERIVLVDTPDAAGTIAPSDPATTVVAAGEPIGVDAAGCAVVLAVPREAVAGVMAWLT
                     PMLAPDAMICVVSANQATTLTVVAEHWPAGQVFGLHPLFDSSARSAEGQTFLIVGEAE
                     RDVTAGWLADLIALGGGISDSGSAADHDAIMGYVQTMTHQALIAFADAVTSSGLDLEH
                     VWEARTPLFEGLFGLAVRALTERQQATIADIQLSMDGPGAAGELGAATARVREVVDGA
                     SPVAIEAMIAGIRDRFSGGLFETIQATAVSAVTATQSKRAELARHRRLGSLVGVRPIG
                     RPQALRVGHIAEVTPVSVTLREVMVGKAGSATLLEGAGRRNAGRVGMNGTPSLTVFGL
                     GHIDVVLGDELEAALDEWLAFIRRDVRFLVPESVAGVGVLTIVSSHAGVRDAEVVSEV
                     VRTGQRSVVVRVFIRADHDVEDTVEALRAQVQATYRWPQGLSLAAPDVQRVHHLGPAG
                     TFSEAAARQATGSLGITAGLVPEPDFTGVLNAIGDGVLGVLPISSSASGLVTRSVTAL
                     LDHPSDLVAGGVVDVAVRFDAYVQESRQLTDLRGAKVFSHPQALGQCQAFIRRWGLDP
                     VPCPSTAEALTMVAADPEGSVALAVADSEAPGLKIAEREVDDLSGSITRFLILGTSGA
                     FGDMTGGWDPTLRSLWVADSVATVLDMIEPGSPAFDELLTDGDGRCLWVTSRRFDDGA
                     PAGARYLGRAPWSPRTPVVRVEVDVPRDRA"
     gene            14558..>16506
                     /locus_tag="PKY13_03360"
     CDS             14558..>16506
                     /locus_tag="PKY13_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOT78995.1"
                     /translation="MSSENRRRAIEAVTKYVAPSEDYSEPLGDLFGRNVFSKSVMKAR
                     LPKTVYKSLLNTIEHGAILDTTVADIVASAMKDWAIENGASHYAHVFYPLTGLTAEKH
                     DSFLEPDGSGASFAEFAGKTLVQGEPDASSFPNGGLRGTFEARGYTGWDVTSPAYILE
                     NPNGNTLCIPTVFISWTGEALDKKTPILRSQQALNIQAQRVLKLFGHTDIDTVVSYAG
                     AEQEYFLVDRNFFFARPDLMSTGRTLFGAPSPKGQEFDDHYFGAIPERVLAFMIDVDR
                     ELFKQGIPAKTRHNEVAPGQFEIAPVFERANLAHDHQQLMMTTLRRVAEKYGMECLLH
                     EKPFAGINGSGKHVNFSIGNSTQGNLLDPGHTPHENAQFLVFCAAVIRSVHKYGGILR
                     AVVASSRNDHRLGANEAPPAIISIFLGEQLMDVFDQIAKGGATASKEKGTITIGADSL
                     PVLPADPGDRNRTSPFAFTGNRFEFRAPGSSQSISGPMVAINSIMAESLDFIATELEA
                     AVAAGTDFNVAVQRVLESIITTHGNVVFNGDGYSDDWQVEAASRGLLNLRTTVDALPQ
                     LDTPEAKDLFEKYGVLSNRELASRMEVYLEQYVLSIQVEAKQTLEMAKTLILPAAYRY
                     QAELAVGAAALKKLGTEYDSSLLDSV"
BASE COUNT         2605 a         5956 c         5564 g         2381 t
ORIGIN      
        1 cgccgtggtg ttctcgtact acgtgatcga gaagcccgcg atgcgcctcc gcaagcggtt
       61 cgcccacgac ggaccaccga cacctgcgcc ggcaccgctc gccgtggccg accagtaggc
      121 cgtcgtcacc cgaacagcgt ctgcgttctc gtctcgaggt cgaggaggta gcgcttggcg
      181 tcgagtccac cgccgaagcc ggtgaggctg ccgtcggatc cgacgacgcg gtggcacggc
      241 acgatgatcg agatcgggtt gcgcccgttt gccgcgccga cggcgcgcac ggcggccggc
      301 gacccgatcg tgcgggcctg ctcgccgtag ctgcgcgtct cgccgtaggg gatacggcgg
      361 agcgctgccc aggcgcgcag ctggaactcc gtgccgaccg ggtcgagggg gacgtcgaac
      421 tcggtccgct cgccggcgaa gtactcgtcg agctgcgcga ccacgtggtc gaacacggcg
      481 gccacgtccg gcggctcgtc gccgatcggc ggcccgtcgg cgggggcacg ccctccggcc
      541 tcctcgggcc aggtcagcga gcgcaggccc tgctcgctcg ccgtgagcgt gagctcgccg
      601 agggggctcg cgtagatccg tcgatgcagg gtggtcgatg tcggtgccat caggctgctt
      661 ccttctcggt ggtggtcgag tcgtccgccg cccagaggtg gtggacgacg gtggtgcgcc
      721 acggggccca ctcgagggag cgcgccgaca gctcggacgg cccgatcccg agtcgagcgg
      781 cccgctgccg gacgacgagg tcgccgccgg ccatgacgtc gggatcgccg agcgcccgca
      841 tggcgacgta gtcggcggtc cagggaccga tgccgggaat gccgagcagg acctggcgcg
      901 cctcggaccg gtcggcgccg gggtcgaggc ggagccgtcc cgacgccacg gcgtcgccca
      961 gctgcacgag cgcggctgcc cgctggcgag gcagggggag cgactcgggg tcgagcgccg
     1021 cgagcgcatc gggcgtgggg aacaaccgat ccagcccggt gtcctcgaac gacagttcct
     1081 cgcccagctc ggcgacgaac cggccggcgg aacgaatgga cgcagcgacc gagacctgtt
     1141 ggccgagcac ggcgcggacg gccatctcga atccgtcgac ggcccccgga gagcggagac
     1201 ccggacgagc tgcgaccagg ggcgccaggg ccgagtcgag ggagagccgg gcgtcgatcg
     1261 cctcgggatc ggcgtcgagg tcgagcagtc ggcggcaccg agcgaccgcg gcagtgaggt
     1321 cacgcacgtc ggcgagacgg agcacggcgt cgacgtgacc gtcgccgggg gccagcgcga
     1381 cgacaccgtg gccgagcggc aggtcgagcg tccgccggta cacctcgacc gaaccggaca
     1441 ggtcgaacga ttcggtgccg gcaatgcagc gggcccggag gaactcccac agcgccgggg
     1501 ccgccaaggg gcgtcggtgt gccaggcgga gcgacaacgt gttcgcagca cccgttgcgg
     1561 cagagcgctc ggcggggcgg cggcgcgccg cagcgacgcg caactcggtg gggctcgcgg
     1621 cgaatacctg ccgcacggtg tcgttgaact gtcgcaagct ggcgaacccg gcggcgaacg
     1681 ccacgtcgac catcgggatc gacgtcgtct cgatcaggac tcgggcattc tgcgcccgtt
     1741 gcgagcgggc cagtgccaac ggcccggcgc cgagctcggc cagcaacagc cgattgaggt
     1801 gacggtcgct gtagcccagg cgccgggcca gcgctggaac tccctcgcgg tcgaccactc
     1861 cgtccgcgat cagtcgcatg gcccgtgcca cgacgtcgga ccgaccgttc cactccggcg
     1921 agccgggcga cgcgtcggga cggcaccgct tacaggcgcg gtagcccgcc aactgcgcgg
     1981 ccgctgccgt cgggaagaac tcgacgttcg ccggcttggg agtccgcgcc ggacagctgg
     2041 gtcggcagta gatgccggtc gtgcggaccg ccgtcacgaa acacccgtcg aaacgggggt
     2101 cccgaccctg gacgattcgg tagcagcggt cgtggtcgtc gaacacgacc ccatcatcgt
     2161 ccggtcagac gacggacgtc agcggttttc ggacgggact attcgacgtt cgctggtccg
     2221 gggacgatga ccacgggaca ggtcgcgtgg ttggcgacct ggttcgtgat cgaaccgatg
     2281 agcaggccga ggaacccacc gtggccacgc ttgccgacga ccaggatctc ggcgccttcc
     2341 gactcctcca cgaggatctt cgcggccggg ccttcccgga cgaccgactc gagcgtgatg
     2401 ccttcggggg gcgtgatcgt ggtgagcgat tcggcgagac cctgctcggt cgcctcctgc
     2461 atcgagtcgg cgggcggcag gccgccgccg gcgaggccgt acccgcccac cgcggtcggg
     2521 gcgtagttcc acgccatcac gaccttgatc gttccgccgc cgagccgctg cacgtggtcg
     2581 aacgcccact gcagggcggc ggccgagttc tcggagccgt cgaatccgac gacggcgtgg
     2641 tgggtgattg cctgcgcttc ggtcatgccg ccatcatggc ccagccgggg ccgtcaagcg
     2701 gggcagtcgc cggtgatgcc cttggccgtc agcacccggg cgacggaccg atcgacctgc
     2761 gccatcggga tgcggccgtc gttgatcgcc gcgatcacgt tgagctgggc ggtcgggacc
     2821 gattcggcga ggctgacgag cgcgatgtcg gcgccggcca tgatcgagag gacagcagcg
     2881 ccggcgttgt cgtagttcgc cgcgatggcc cccatgccca acgagtcggt gatcacgagc
     2941 ccgtcgtact tctcccgccc gcgcagttcg tcggtgatcg cggcccgcga caacgaggcc
     3001 ggcatcccgt cggtcaggcc ggggatgatc gcatggctca ccatcagggc gtcgagaccg
     3061 gcggcgatcg cccggtcgaa cgggatcagg tcgcgctgct tcagggcgtc gagctcgtcg
     3121 acgggcgacg ccgaatcgtg tgggtcggcc tctcccccgc cgatccccgg ccagtgcttc
     3181 gccaccggga tgaggcctgc cgccttcacg gcaccgatga ccgcgatgcc gtaggacgac
     3241 acctgatcga cgtcgtcgcc gaacgaccga gttcccagac ccgagccgcc gccgacgtcg
     3301 aggctgggac cgaagttcat gttgaggccg agggctcgga gcttcgcccc gtactcgccg
     3361 aagatctggg cggccttctc ggggccgttg tcccgggcga gcgcggccgc cgacggcaac
     3421 gcgccgagca tgtcgcggaa ccgttgcacg gtgccgccct cctcgtcgcc cgaggcgaac
     3481 accggcagct tcgaccgcgc ggccacggcg gcgacctccg cggtgacgtc accggtctgg
     3541 gcgccgacga gtgcgtagcc cgcgacccgg ccggcagcga cgaggtcgcc ggcggcggcg
     3601 ggcgtgggga ccagcaccat caggagctgg ccggccttct gctccacgga cagggccgcg
     3661 acgcacgccg ggaccgcgat ggtggtcgtc gtcaccgtcg acgacggcga tatcgcaccg
     3721 gtcgccaccg ttgcgtcggt cgccccgaca cacgcagcgg agacgagcaa cgcacccggg
     3781 gcgagcagcc tcaccgtcaa cgaacgaaac gacacggcgc agaacgtaac ccgtggggcg
     3841 cctcgccgaa atgccgatcg atgagatcag gccgagccgg ccacgcccca ctcgccacgc
     3901 agcccctcga cgaactgggc gaacgccggg ccctcggcct cgatctcggc gagcacctgc
     3961 ttcaccacca tgtggtggaa ctcgtcggcc gccgggggcc ggagcggaag ccggcccgcc
     4021 tcgtgctctg cctcgacgac cagcccgatc tcgaacaggc ccttcatcac gaactcgacc
     4081 tcctcgggcg atacatcggt cacccagtgg aaggggttgc cggccgaggc ggcgtcggcg
     4141 aactcgtcga gcagggagtg gaaccgatcg agcacgttcg cccgggaggg acggttcgcg
     4201 gcggcgaggt ggtcgacgca acgacggccg tacgcgatcc acgcgaccgc agtgtccacc
     4261 ccgaccggtc cgatctccac gcgcatgact tgatcatggc ccacacgacc cacggcgcgc
     4321 gccgcggccc ggtgcgcgcc aaggtccggc tcagcgaccg cgcacgcgtc gccgagccag
     4381 caccaggagc aggacgacaa cgaccaaggc gcccgctgcc gtccacggcc ctcgccacga
     4441 cgacccgccc gagccgtcag gtcgatcggc gatggcggtc ggtgccggtg ccggtgccgg
     4501 tgccgccgtg gtgctcggcg ctggggcgac agtcggcgcc gcgtccgcga ggaagatcgg
     4561 gttggagatg gtcgtcaagg agagatcgtc gaacgtctgc acgccccaga acgtgccgag
     4621 cggcccctcg tcgtcggtac ggtcggcggt gaacgagtgg acgaagggcg aggaggtgac
     4681 gtccacgacg gcggcctcga cgccgttacg caggacacgg aggcgctggc ctgccccgtc
     4741 ggtcacccgc accgtcatcg tgctggtgtc gcccacgacg gtgtcgccca tcatcacctc
     4801 acctccctcg ggtgtggtcg ccgtcaactc gagagagggc gatccggacg caccacgggt
     4861 ctggacgtac gcatggccgg cccgaacggc gcgttgcagg gccgggcggg acaactcgtc
     4921 ggcgtacacc accgtcgcca cgctgccgag gtccgggccg agcttgtcgt cggagccgct
     4981 gaccgcggtc acccggtgcc cgctgacgag caggttcgtc cagaagtcga tggccgtggc
     5041 cacgaacggg ttcggggcgg ttccgtaggc cactgcgtcg gtcacgacct cgacggtgtc
     5101 gacccggtcg gggtcgatcg tgtcggagag ctggaactcg cagccccggc acgtgctgcc
     5161 gaagtcccgc tccgggaaga ccgtgggatg ggcgatcccg aagagcgcac cgtcggcgag
     5221 cacgtcgtcc tggatcgagc ccagcgtcac gtcctcgaac ccatgtcggt actcgaccgt
     5281 cgatggcgtc tcaccgagga cgatggcgtg cccgaagtag gtgatgacct cacgtccggg
     5341 ccagatgagg aggtcggggt tcgcccgctg catctccccc cattcgttcc agtgacgatt
     5401 gacgacgtat tcggtgaccg ggaggaagtc gaggttcgcg tcgcgggcgt attggacgaa
     5461 gccttcgttg tcgggggcct cggggttgga gtggaacccg tgcatgtgga agtcgccccg
     5521 gtaccacccc ggatccgggt cggcgacgtg ggacgcatcg accgggtcgg cgtcgatctc
     5581 ggggccgacc gggtcatcgg tgcagcgcag ctcgacggtc caggtggcgc cggcaggcgc
     5641 cacctcagcg aaccccagct cgacgtacca cgtgccggcc tccacggtgc cgggcgtgaa
     5701 cgctcgctcg gcggagtccg cctgcaccca cgcccgggac atcccctgat cgagtcgacc
     5761 ctcgcggctc ccaccccatc cgcggaaccc ctcgggtgag cggtagccgg cggcgtccca
     5821 caggccgaga tcgagcgtgg tgccctcgct cggcgtccac gagtacccga cctcgacccg
     5881 ggtggtgccc gtggccacct cgaacggctc cacccgatag gtcttcgcga ggtcgggggt
     5941 cacggtgccg gtcaacgtca cgacgccgac atccggagcg cacacctccg gagcgagcgt
     6001 cgttgcgccg gtggcaggga gtgtcagcgc gagcgcaacc accgccatca gacctcggac
     6061 tgcgatcacg ggagctcgga tcgtccgagg atctcgccgt tgcgcatgac catgacggga
     6121 ccgtcctcgc tgatcacgat caaggtcgcc gtcgggtcgt cgaagctgta ccgccgcgcc
     6181 gaagtgtgtc gggtgccccg gacggcgtcg acatccgcct cggcctcggg actcgggacg
     6241 agccgcaccc cgagctgacg gagggtgccg acgtcgtcga acacagcggc accgtcgacc
     6301 tggccgagca cgtgacgaag gggcgccagg tcagccggac ggttgatgtc gagcggcggt
     6361 ggctcgggga gccggacctc ccaggtcggc aggtcgggtg cgagcggttg gtagatgagc
     6421 agggcaccga tgccgcgggc gcccagatcg tgcacggcga actcgaggag acgacccagg
     6481 acgtcgcggt cgccgtgctg gcggcacgcg gtcatcgcgt cgatccagtc cgacacgggc
     6541 gattcgcggt gccagctcag accgtcccac cggaggaccc cgacatcacc gacgatgcgc
     6601 acgaggcccg atgggtgacg ttggacgatg gtcgcatcca tgacctgggc catgaccacg
     6661 agatcacgtt ccgaaccggc ggggcggtcg aacacggcga gctcgaggtc gcggtcgccg
     6721 cggcgaacca gccagctcga cagaccgtcg gcgaatcggc gggcatcgcg cgccgggaag
     6781 ccctcgagat gccgatgggt gacggagaga tcggtggccg cgtcccagcc ggaggggtcg
     6841 gctccgggct cgacgatcgc cccgaagctc gggatccgcc gctcgtgcac cgccggccgg
     6901 agcgcgtagt cgagctcggc gaggagcacc gagccgaccg gatcggtcac gtcgatcgcg
     6961 aacccctcct cggccagctc ctcggtgagt cgccgcaggc gcccggcgac agagcgttcg
     7021 acggtcatgc ccgcacccta ccgagtcgtc ccgacacctg acccggggag ggggcagggc
     7081 cggcagtcca cgaacgtcga gcttcgggta tgaagaacgt catgtcggcg tcgcggctcg
     7141 cctgagaaca gcccgaggaa gcctcttgac gtcacccgta tgatggagtc cggtccgctg
     7201 gagctggacg tccgcattcg cgtgcaggcc ccaaccacga tgaccgtcga tccgaaacga
     7261 atgaggtgtg tccagtggcg aagcagcgac cagagcgcga tgaagaagat ctcgtccgac
     7321 tctatctgag cgacatcggg cagtacccgc tgctcaccaa ggacggtgag gtcacccttg
     7381 ccaaggccat cgaggccggc gtggccgcac gcaccgagtt cgaggcgaac gagcgcacgc
     7441 tcaccgctgc ccgcaagcgt gagttgcggc gcacgatccg cgccggcgaa gaggccgagc
     7501 tggcgttcgt ccagtccaac ctccgcctcg tggtgtcgat cgcgaagaag taccaggcgt
     7561 cgaacctgcc gctcctcgac ctgatccagg agggcaacct gggcctcatc cacgccgtcg
     7621 agaagttcga ctggcgcaag ggcttcaagt tctccaccta cgccacgtgg tggatccgcc
     7681 aggcgatcac ccgcggcatc gccaacacgg gccgcaccat ccgcctcccc gtgcacgccg
     7741 gcgacgtcct gtcccgtctg cagaaggccc gcttccgcct cgagacgagc ctcggccgtc
     7801 cggccaccct cgccgagctc tccgtcgagc tcgacatgcc gatcgccaag gtcaccgagg
     7861 cgatgcggta cgccgccgac acgctgtccc tgtccgagcc cctccgggcc gacggcgacg
     7921 ccgagctggg cgacatgatc gaggaccgcg gcgccgagtc gccgttcgag tccgccgcgg
     7981 tggcgctgct ccccgaccag atcaaccgtc tgctgttccc gctcgacgag cgtgagcgtc
     8041 agatcctcaa gcttcgcttc ggtctcgacc gcggcgagcc ccgcaccctc gaagaggtgg
     8101 gcgagcactt caacctcacc cgtgagcgca tccgccagat cgaggcccgc gccatgtcga
     8161 agctgcgtca ccccagctcc gataccggcg ccaaggatct gctcgccgtc tgagccgacg
     8221 ggtagcgata cccagcacag ctgaggcgtt cgcctcaaga actcctcaag aacccgcccc
     8281 cgaggtcgat gggaaccggc gtcgccacaa ccgtggcaac cgctggaacc atgtgactct
     8341 cgggggcgtt cttgttcgtc ttcctgctcc aggtcaggga caccctggcc aatggccgcg
     8401 tctacctctt cacgatcttc gtgacgctgg tgtgggtggt gtggctccgg cgcgtgcagc
     8461 tgtcgcgtcg gtatcggccc tacaccgagg acgtctcgct gacgaccagc gtgatcatcc
     8521 cggtcgtcga cgagcccgag gagctgttcg acgaagtgct ccggcgcatc gtcgcccaag
     8581 gccccgacga ggtgatcgtt gtcatcaacg gcccgcggaa cgagccgctc gaacgcatct
     8641 gtgaacgctt cgaccgggtg atctggcgct ggaccgaagt tccgggcaag cggaacgcac
     8701 ttcgcgtcgg ggtcgacgcg atgctcggcg atatcgcggt gctcgtcgac tcggacacga
     8761 tctggacaac agggacgctg cgtgagctgt tgaagccgtt cgccgacgag cgggtcggcg
     8821 gggtcaccac ccgccagcac atcctcgccc aggagcgcca cgtgctcacc cggtgggcgg
     8881 actggatgga aagcgtccgc aacgagtact cgatgcccgc gatgagtgtg ctcggcacgg
     8941 tcggttgcct gcccggtcgc accatcgcgt tccggcggca gatcctcgtc gacgcgatgc
     9001 ccgccttcct gtcggagcgg ttcctcggca tcttcctcga ggtgtccgac gatcggacgc
     9061 tgacgaacga ggcgctcaag cagggctacc gcaccgtcta ccaggcgacg tcggaggtgt
     9121 acaccgacgc accgaccacg tggaagaaga tggcgaagca gcagctgcga tgggctcggg
     9181 gcagccagta caacaccgcc cggatgctcc cctggatgct ccgcaatgcc cggaccctcg
     9241 cgttcttcta cgtgtgcgac atcgtcctgc cgttcctcct cgtctgcacg atcaccggct
     9301 gggtcttccg ggcgagccgt gtcgactccc ccgacatcta cggcgcgttc gtcgcgcact
     9361 acggccgctg ggtcgcgctc ggcctgatga tcacgttgtc ggtcgtcatg agcacggcgt
     9421 cgtcggcaat ccgccaggcc cgccacctca aacgccgccc cgacgatctg ctccgtctgc
     9481 cggcgttcat cctcatcagc accttcttcc tcatgcccat ccgcctgttc gggttcacgc
     9541 gcatgggaca caacagcgga tggggcaccc gatcgggcgg ctactcgggt gaacgcaacc
     9601 ggaaccccct cgccgccatc ccctacctga tcgccgtcgc catcgtggtg gccggcaccg
     9661 tcctcccttc ctgacacccc gccccgacct ccaggaccac cgatgccacg ccacctccgc
     9721 tccacaccga gcacacccga caccgaaccc accgcggtgc cggccgccgg gtccgagctg
     9781 ccgatccacc tcgtcgcgac gccggtcgcc cgtgggctcc gccgcctcgg cgtcggtacc
     9841 gtgccgttca ccatcgtcgc catggtcacg ctcgcagcga tgtcggcctt cgtcgtgatg
     9901 agtccgacgt ccatccccgg cctcggcgca cccgacgagc tcgcgagcgt cgacctcggc
     9961 gtcggtatcg acggtgcacc caaccggtcg ctcgatcccg acgtcaccac gaccatttcg
    10021 gtcgcggcga ccaccacggc gtcgacggag ccgtcgacaa caaccgccac gccacaggcg
    10081 acctcgcgcc cgacgacgtc gaccgcgccg ccgtcatcgg cgaagccggc gagcacgccc
    10141 accggtgagc tggcctcgac gccggtctcg gtgcggccgg ttcggtgcga ggacttcacg
    10201 ctgcagcccg aggcgcagct gtccttcgag gccgacccca tcggccgcgc cggactcgac
    10261 ggtgacgccg acggcatcgc ctgcgaacag ctgcccgggc ggacacgcaa cgcgctgccc
    10321 gccctgtcgg cgcggcggat cccgaccgtc gagcaggtac gacgcccggc gacgcggctc
    10381 ttcgggctcc acacacctca ggcgcccttc gccacgtcgg agatcgaagc cttctcggtc
    10441 gccgccgaga agacccccaa ctcgctgctc ttcttccaga acttctccca ggagttcccg
    10501 ctgtcggcgg tgacgacgag ctggagccgc ggcatgctgc cgatggtcac gttcgaaccc
    10561 atcatccagg acagcccatc gggccaaccg atgttggccg acatcgccga cggggtgtgg
    10621 gacgagtact tcacccggtg ggccactgcc gcgaaggcgg aaggtcaccc gatcgcgttc
    10681 cgcttcgcgc aggagatgaa cggcaactgg tattcatggt ccgacggtcg gttcggcaac
    10741 gctcccggag atttcgtccg ggcatggcga cacgtccacc agatcttcga cgacatcggt
    10801 gccgacaacg tgctgtgggt gtggagcgtc aaccgggtcg acaacctgcc tgacaagacc
    10861 atcgcccggg tgtaccccgg cgacgagtgg gtcgactggg tcggcgcgtc ggggtacctc
    10921 cggaacgtgc ccgacggggt cacccccacc ttcgagtaca cgttcgggca gacgctcgat
    10981 gcgatcgagg cggtggcgcc gaccaagctg gtcatgctca ccgaggtcgg tgccggcacg
    11041 accgaatcca accgggtgag ctggctgtcg tcgttcttcc aggggctgct cgaccgcccg
    11101 gagatcatcg gattcaattg gttcaacgac ttcaaggacg gcggcgactg gcgcatccag
    11161 tactcgacgg ccacgtcggc agcgttcgcc gccggggtcg ctgaccagcg ctacggcgac
    11221 ctggctcccc tcctcccggc atgacgaccc gcagcgacgg atgatggcat gaagaagagt
    11281 gctcatcatc cccctccaac ggtcgatgtg agaggggtgc cgcgtcctcc tgaggggtgt
    11341 cggcctgcca cagcgacgcg tcgccgctgc caccagctcc ctacgtcagg accatggtca
    11401 tgaagtcatt cgcccgcaac cgcaagaaca agcggaccga cggcatcgac cccgacaccg
    11461 ccgagggtct catcgacgtc gacgagctcg acatcgcccc gctcggcgtc gcccccggca
    11521 cgcggtcgat cccgggcagc gatgacccga acgccgacga ggcggcggac ttcgcggaac
    11581 gcctcggcga cctggatcgc ttcatcggcg ggggcaccgc tcccgcaacg ggcccgacca
    11641 cgtccgtgtc ctggacgggt ggggcgccgg cgcgcaacct gcccgctgcg ccgatccccg
    11701 ccgtcgctcc catcagccac ccacggccgg cgtccttcga ccgcgcggcc gagctcgagc
    11761 cccaatccgg cgccgtcgag cggcccgtcg ccgaccccga ggccgaggcg caggagttcg
    11821 tcgaccggct gggcgatctc gaccgcttca tcggcggcaa cggtggcgca ccgacacccg
    11881 cggcggcgac cacacccgag atccccgtcg ccgaccccga cgccgaggcc caggacttcc
    11941 tcgaccgact gggcgatctc gaccgcttca tcggcggcaa cggctccggc gggaacgacg
    12001 ccgacgagca gcgaccgcac cgagactgag gcgtattcga ccggggcaac gccgccccgg
    12061 cgctagcctc cgacccatga cgaacccggc ctcaggggcg ccgatcgacg cgctctccgg
    12121 ccctgctcgg cccagctcac tcgtggtggt cggcgccgcc gccggcatgg gccgatggct
    12181 caccgatcac ctcctcctcg accggcactg ggagcggatc gtcctcgtcg acacccccga
    12241 cgccgcgggc acgatcgcac cgtcggatcc cgccaccacg gtcgtcgccg ccggcgaacc
    12301 gatcggtgtc gacgccgccg gttgcgctgt ggtgctcgct gtcccgcgcg aggcggtcgc
    12361 cggagtcatg gcgtggctga cacccatgct cgcccccgac gccatgatct gcgtcgtgtc
    12421 ggccaaccag gcgaccaccc tcaccgtcgt cgccgaacac tggccggccg ggcaggtgtt
    12481 cggcctgcac ccgctgttcg actcgtccgc ccgatcggcg gagggccaga cgttcctgat
    12541 cgtcggcgag gccgaacgcg acgtgacggc gggatggctg gccgacctca tcgccctcgg
    12601 cggtggcatc tcggactcgg gatcggccgc agatcacgac gccatcatgg gctacgtcca
    12661 gacgatgacc caccaggcgc tgatcgcgtt cgccgacgcc gtcacgtcgt cggggctcga
    12721 cctcgaacac gtgtgggagg cacggacacc gctgttcgag ggcctgttcg gcctggccgt
    12781 cagagccctc accgaacgcc agcaggcgac gatcgccgac atccagctct cgatggacgg
    12841 ccccggcgcg gccggcgagc tcggcgctgc gacggcgcgc gtgcgcgagg tcgtcgacgg
    12901 ggcgtccccg gtcgccatcg aagcgatgat cgccggtatc cgcgaccggt tcagcggcgg
    12961 gctcttcgag accatccagg cgacggcggt gtccgcggtc acggcaaccc agtcgaagcg
    13021 tgcggagctg gcgcgtcacc gtcgcctcgg atcgctcgtc ggggtgcgcc cgatcggtcg
    13081 gccccaggcg ctgcgggtcg gccacatcgc ggaggtcacc ccggtctcgg tcacgctccg
    13141 agaggtgatg gtcggcaagg ccggatcggc caccctcctc gaaggcgctg ggcgccgcaa
    13201 cgcgggacgc gtcggcatga acggcactcc gtcgctcacg gtcttcggcc tcggacacat
    13261 cgacgtggta ctcggcgacg aactcgaggc ggcactcgac gagtggctcg ccttcatccg
    13321 acgagacgtt cgcttcctcg tccccgaatc ggtcgccggc gtcggggtgc tgacgatcgt
    13381 gtcgagccac gccggggtgc gtgacgccga ggtcgtgtcg gaggtggtcc ggacgggcca
    13441 acgcagcgtg gtggtgcggg tgttcatccg tgcggaccac gacgtggagg acacggtcga
    13501 ggccctgcga gcgcaggtgc aggcgacgta ccgctggcca caaggcctgt ccctcgccgc
    13561 ccccgacgtg cagcgcgttc atcacctcgg tcccgccggg acgttctcgg aagccgccgc
    13621 tcgacaggcg accggcagcc tcggcatcac cgccgggctg gtccccgagc ccgacttcac
    13681 cggggtgctg aacgccatcg gcgatggggt gctcggggtg ctcccgatct cgagctccgc
    13741 atcgggcctc gtcacccgct cggtcaccgc gctgctcgac cacccgtccg acctcgtggc
    13801 cggtggcgtg gtcgacgtcg ccgtgcgctt cgacgcgtac gtgcaggagt cgcgtcagct
    13861 caccgatctc cgaggcgcca aggtgttcag ccatccccag gcgctcgggc agtgtcaggc
    13921 cttcatccgc cggtggggcc tcgaccccgt gccgtgtcct tccacagccg aagcactcac
    13981 gatggttgcc gccgatcccg aaggctcggt cgcactggcc gtcgccgact ccgaggcccc
    14041 aggcctcaag atcgccgaac gcgaggtcga cgacctgtcg ggatcgatca cacggttcct
    14101 catcctcggc acgtccggcg cgttcggcga catgaccggc ggctgggacc cgaccctgcg
    14161 gtcgctctgg gtcgccgact cggtcgccac tgtgctcgac atgatcgaac cgggctcgcc
    14221 ggcgttcgac gagctgctca ccgatggcga cgggcgctgc ctgtgggtga cgagccgtcg
    14281 cttcgacgat ggcgcgccgg cgggggcacg atacctgggc cgggcgccgt ggtcgccacg
    14341 cacgcccgtg gtgcgcgtcg aggtcgacgt tccccgcgat cgcgcctgag aacggccgag
    14401 gcggacgctg tcggggcccg gtccgtcccc aagctgtcat tccgccgaaa cactcccgtg
    14461 atcaacgctt tctaggttct ggcgaagcag acgcgtcgcg tggcgcgacc cgatacggga
    14521 gcaacacccc catctgggga tcaacaggga gcaagtcatg agcagtgaga accggcgacg
    14581 cgccatcgaa gccgtcacca agtacgtcgc accgtcggag gactactccg aacccctcgg
    14641 cgacctcttc ggccgcaacg tgttcagcaa gtcggtcatg aaggcccgtc ttccgaagac
    14701 cgtgtacaag tcgctcctca acacgatcga gcacggcgcc atcctcgaca ccaccgtcgc
    14761 cgacatcgtc gcgtcggcca tgaaggactg ggcgatcgag aacggtgcgt cgcactacgc
    14821 ccacgtcttc tacccgctca ccggtctcac ggcggagaag cacgacagct tcctcgagcc
    14881 cgacggcagc ggcgccagct tcgccgaatt cgccggcaag acactcgtgc agggcgaacc
    14941 cgacgcatcg agcttcccca acggtggcct ccggggcacg ttcgaggccc gcggctacac
    15001 cggctgggac gtgaccagcc ccgcctacat cctcgagaac cccaacggga acacgctgtg
    15061 catccccacc gtgttcatct cgtggacggg cgaggccctc gacaagaaga ccccgatcct
    15121 ccgcagccag caggccctca acatccaggc acagcgagtg ctcaagctgt tcgggcacac
    15181 cgacatcgac accgtcgtgt cctacgccgg ggccgagcag gagtacttcc tcgtcgaccg
    15241 caacttcttc ttcgcccgcc ccgacctcat gagcaccggc cgcaccctgt tcggcgcgcc
    15301 gtcgccgaag ggccaggagt tcgacgacca ctacttcggc gccatccccg agcgagtgct
    15361 cgcgttcatg atcgacgtcg atcgcgagct cttcaagcag ggcatccccg ccaagacccg
    15421 ccacaacgag gtcgcccccg gccagttcga gatcgccccg gtgttcgaac gcgccaacct
    15481 cgcccatgac caccagcagc tgatgatgac gacgctccgt cgggtcgccg agaagtacgg
    15541 catggagtgc ctcctgcacg agaagccgtt cgccggcatc aacggctcgg gcaagcacgt
    15601 caacttctcg atcggcaact cgacccaggg caacctcctc gacccgggcc acacgcctca
    15661 cgagaacgcc cagttcctcg tgttctgcgc cgccgtcatc cggtcggtgc acaagtacgg
    15721 cggcatcctc cgcgccgtcg tggcgtcgtc ccgcaacgac caccgtctcg gcgccaacga
    15781 ggcaccgccg gcgatcatct cgatcttcct cggcgagcag ctgatggacg tgttcgacca
    15841 gatcgcgaag ggcggcgcca cggcgtcgaa ggagaagggc accatcacca tcggtgccga
    15901 cagcctcccc gtgctcccgg ccgaccccgg cgaccgcaac cgcacgagcc cgttcgcgtt
    15961 caccggcaac cgattcgagt tccgggcgcc cggttcgtcg cagtcgatct cgggaccgat
    16021 ggtcgcgatc aactcgatca tggccgagtc gctcgacttc atcgccaccg agctcgaggc
    16081 cgcggtcgct gccggcaccg acttcaacgt cgctgtccag agggtgctcg agagcatcat
    16141 caccacccac ggcaacgtcg tgttcaacgg cgacggctac tccgacgact ggcaggtgga
    16201 ggcggcgtcc cgtgggctcc tcaacctccg caccaccgtc gacgccctgc cccaactcga
    16261 cacccccgag gccaaggacc tgttcgagaa gtacggcgtg ctcagcaacc gtgagctggc
    16321 cagccgcatg gaggtctacc tggagcagta cgtgctcagc atccaggtcg aggccaagca
    16381 gaccctcgag atggccaaga cgctcatcct gccggcggcc taccgctacc aggccgagct
    16441 cgccgtcggt gcggcagcgt tgaagaagct cggaacggag tacgactcgt cgctgctgga
    16501 ctcggt
//
LOCUS       DAOSOA010000027        16471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3550_length_16471_cov_5.589790, whole genome shotgun sequence.
ACCESSION   DAOSOA010000027 DAOSOA010000000
VERSION     DAOSOA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16471
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3550_length_16471_cov_5.589790"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PKY13_03365"
     CDS             complement(<1..580)
                     /locus_tag="PKY13_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOT78996.1"
                     /translation="MAVEPRNLVFFPTPGDLRAWLVEHHQRETELWIGRYKKGSGKPS
                     VTWPEIVDQVLCFGWIDGVGKGIDDEVYAQRITPRKAGSIWSAVNIAKAAAFTEAGLM
                     YPAGLAAFEARREDRSAIYSHEQDEPATLSVEQEAVLRSVPGAWEFWESSAPSYRKAA
                     THWVTSAKREETRASRLATLAEGSAAGLRVKPL"
     gene            complement(814..2604)
                     /locus_tag="PKY13_03370"
     CDS             complement(814..2604)
                     /locus_tag="PKY13_03370"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT78997.1"
                     /translation="MTTPEPSAIESAKERYEAERAKRLRSEGLAQYESLRDHDLDRDP
                     WADPDFTRDPVVEDVQVVVLGGGWAGMLAGINLVKRGITNFRIVEKAADFGGTWYWNR
                     YPGCMCDVDSTIYLPLLEETGYMPTEKYASASEIFGYAQLLGRHFGLYDHALFQTEVE
                     SLVWDDDAHRWDITTQRGDRIRTQFFVAAGGLMHKAKLPGIAGIDDFTGKAFHTTRWD
                     YEYTGGSPTEPMDRLADKVVGIIGTGATSVQIVPQLARSAKEVYVFQRTPSAVGVRAQ
                     QQIDEEWFRSLKPGWQLERTLNFTSVATGQQPDRDLVADGWGQVLKVDTQREPADDAE
                     RAELEAIDFEIMEGFRRRIDEVVEDPETAAKLKPWYGKHCKRICFHDEYLQSFNLPNV
                     HLVDTDGQGVREMSPAGPIVDGTEYPLDLLVFASGFEITTGLVDRLGFDPVGRGGVKL
                     SERWHDGTHSLHGILTAEIPNFFVVSFVQAGFGLNFVHFLSESTKHIVWMIDHCLEEG
                     IASIETTVEAEDEWLNILWQASGPLARYNLACTPSYGNSEGARTMLSARSAVFPGPLM
                     DYAAHLERWRDEGTLAGAKIVRAEESSIEP"
     gene            complement(2876..4096)
                     /locus_tag="PKY13_03375"
     CDS             complement(2876..4096)
                     /locus_tag="PKY13_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOT78998.1"
                     /translation="MPDALIIDACRTPRGIGKQGKGALAHLHPQHLGRTVLEALAQRN
                     DFDTSDVDDVIWGTSSQVCEQSGDLGRMSALDAGYDVRASGVTLDRFCGSGITATNIA
                     ANAIMAGMEDLVVGGGCEMMSLPKKGLLPMGAGNAHLQEIHPQSHQGVCADAIATLEG
                     IDRGTLDALAAESQARAARAIAEGRFDKSLIPVLNLDGTVALDQEQFPRPGTTAESLA
                     ALPASFPQIADYRHNDNPTFRELINQKYPDLDIAHVHHAGNSSGVVDGAAAILLASEG
                     YAKAHGLTARARIVATANMGDDPTLMLNAPVPAARKVLARAGLTLGDIDLFEINEAFA
                     VVAEKFMRDLDLDRDKVNVNGGAMALGHPIGATGAMLIGTVLDELERIDGQFGLITMC
                     AGGGMAPAVIIERV"
     gene            4156..5577
                     /locus_tag="PKY13_03380"
     CDS             4156..5577
                     /locus_tag="PKY13_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT78999.1"
                     /translation="MTEAVVVGSGPNGLAAAITLAEAGLAVRVLEAADRPGGGVRSSE
                     ATLPGLIHDDGSGFHPMGVASPFFQAQDLDRFGLEFLWPEIELAHPLDDGRAGLLWRD
                     TERTVAALGIDGARWDATIGWAGRRFDRIGSEILRPIVHVPRHPVVLSRFGALSALPA
                     TAFARVFHTEEAAGLFGGIAAHGFGKLTMPFSSSAGIMLGGAGHAYGWPVAKGGSQAI
                     TDAMVAKLTALGVTVETGVTVTSFDELGSPDVVMLDTSPAAAARILGDRLPSRVARAY
                     RRFRPGPAAYKLDIALEGHIPWSNPDVGRAGTVHVGGTMAEMAAAEADTNEGRMPDRP
                     FLILGQQYLADPQRSSGGLHPIYVYAHVPNGYDRPEGTELILRQIERYAPGFRDLIRH
                     VTVTTPRQLSAANANIVGGDIAGGASSGPQLIFRPRIALDPYDTTVPGVFICSASTPP
                     GAAVHGMGGFNAATRALRQVHGG"
     gene            5715..7118
                     /locus_tag="PKY13_03385"
     CDS             5715..7118
                     /locus_tag="PKY13_03385"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOT79000.1"
                     /translation="MRRTVIGMMAIAATTSLVACVPETPALPPVAGGTGGGDSYFPAM
                     GNTGYDVAHYDLDLDYDPTTKALAATAVIKAATVGAPLSSFSLDLRDLTASSVTVGGR
                     PAAFTQGGGELIITPESPVSGTFVTTVVYGGTMGQPLDGTGALYGWVAFDDGAFVANE
                     PEGASTWYPVNDVPSDKATYRFTITVPDGTTGIGNGDLVSQTSAGGSTTFVWVAADPM
                     ASYLSLAASGNYSTTTETTPSGLPIVNFVDVDLSPGDQAAATAVLAQQPAMVDYFDDM
                     FGPYPFSSFGAVIDDDEDPGYALENQTRPIYAGVPSSSTVAHELAHQWYGNKITPKYW
                     KDIWLNESFATYAEWLWGEHNGGTTVTARYTTVKNRSAANAFWTVVTADPGPDDLFAA
                     ATYQRGGAFLHALRLTIGDTQFFSILRQWADGNANTPVTTADFQALVESVSGLDLDAW
                     FDAWLYQPTKPAAAYLP"
     gene            complement(7136..8386)
                     /locus_tag="PKY13_03390"
     CDS             complement(7136..8386)
                     /locus_tag="PKY13_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HOT79001.1"
                     /translation="MPISHRWLYAAAVLVLAATLTSCGEPALDAFYDAGGPITGRRPG
                     LVDAGSLPGDVVSSRPARFTVDPFTLAPVAGVESQQLLYRSTTGRGEPVVVSGTVLVP
                     TTPWTGAGSRPLVSYGVGTRGVGDQCAPSKSLSGGLDYESGIFQEALGRGWAVAVSDM
                     VGLGTAGTHTYEVGRDQGRALIDIARAAERLDGTGLDASTPVAFWGYSQGGTSAGWAA
                     QLAASYAPDLNVVAVAAGGVPADLRAVAVNLDGNPFFSLLTLAALGYDAAYPGLDLDR
                     YLNDAGRRTFAANRNFCIVGIDGIQTFGSLAGHHMSDYLGSANPLTTPEWIAALDENR
                     LGGAAPAAPVLLLHGLVDQVIPRAQAVTLLREWCAAGATVAWTDLPTEHALGIVLSVQ
                     PALDFLSARFAGVPATSSCGEVWP"
     gene            8539..9090
                     /locus_tag="PKY13_03395"
     CDS             8539..9090
                     /locus_tag="PKY13_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT79002.1"
                     /translation="MTEARHTERGADDPMLSDRELEVMRLLAKGMSNASIAAELYIGV
                     ETVKSHLSGIYRKLDVSSRGQAVAWLNDRPALAIDPDAPIASTTTSEIPGGVRVVLAG
                     EFDLTTMQLLSDALDAARAASTGAVELDMEHVAFIDSSALRVLVGFITREPKIPVRIV
                     NPSPSVTRVVEFAGLKGPFGLAD"
     gene            complement(9243..10919)
                     /locus_tag="PKY13_03400"
     CDS             complement(9243..10919)
                     /locus_tag="PKY13_03400"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT79003.1"
                     /translation="MGSRKVVDRSLATVTLPGGLVPVVDALNDVVVVAELDGSILFAN
                     VATEALLGWSPAELVGRPLVDLVPERLRSAHLAGMSRFVEGGEPRLIGQPTRVPALRA
                     DGNEVAVELLLSMLPTSGGGGYVVATMRDVRQRVDLERDSTLIHHVLDVVSGSAGTPD
                     AIGRLLAVVATTVGWDAAGLWEPDADRSSLRCEQVWFRPGLDASAMSDASSATAFVPG
                     EGLPGRVWTEAAPAWVVDVSVDDNFPRATAAAAAGLRCGLAFPLVDAGKVVAVIELFR
                     TSSTEAEPSLLATLGDLGTSLGGWLTRTRLQDAYDAISERERNVADVLRRTLLPPELP
                     EIDGFEIAAAFQPGADSSVGGDFYDAFPLSDGGGNVAIAIGDVCGSGPEAAAVSGQVR
                     HTFRALAMWATSPAGLMESLDSLLAATIHDGRFCTAVFAMLHVDYAGGVSGRLSAAGH
                     PPPLVIRASGDVDVIELRGPLLGVLPDGRWVDADVGLAPGDTLVLYTDGVTEARDAEG
                     RLFGEAGLRELCAELVGRRPAEIVATIRERALAHAGAITDDIAVLALAPA"
     gene            11014..12357
                     /locus_tag="PKY13_03405"
     CDS             11014..12357
                     /locus_tag="PKY13_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT79004.1"
                     /translation="MEPSPESGGDEPPPSAPEPDTGRSGRAPLVVALALLLVGVGVAV
                     ALVVGGDDSDNTASPTSTTAVTTTIAASTTSGAATAPAAVDPVTGLPTGRFTIVGRQI
                     VDPDGNVFVPMGANVAVRQGGFEEGYAFNWNGTATGRVADVKAWGWNTVRATLVCDLD
                     GTGPTVDELNAGIDAFIEEYTRQRVVVMVECHDITGDNLGPTDPKVQALFPFWDRLAT
                     KWKDNPYVWFNFYNEPQGTTTAAGVDNWLDLQRDALGRLRAIAPTSIFVADIPGAAQG
                     LGTLTGDDPITRLGDGQCNVLYGWHSYGAVGNDDEFGSPNDWDNEAASLANHRKVFEF
                     LRDNDIPMVIGEVGDPLTLDEGNAGQPIWNRYGARSVIALAPEFGVGLLWWHGTGDSG
                     VFLTYSLMAERHEAPWSAATTGEGLSDGGRRFWEASKNQPAPTRFTGDLAAGNCG"
     gene            12440..14071
                     /locus_tag="PKY13_03410"
     CDS             12440..14071
                     /locus_tag="PKY13_03410"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOT79005.1"
                     /translation="MDTTTDSTTARRHGRAVFHIADLFELVAEAIPDRPALVGGDHRL
                     TYGELDERSNRLANHLIANGLEPGATISIYAWNRTEWVESLFGAYKARAIPINVNYRY
                     LADELRYILDDSKSEALVFERSFAPIVAAVIADLPRLRQLIVLEDGSDQPLDGLPGVV
                     RYEDALADASPERVDIERSPDDLYIMYTGGTTGMPKGVMWRNEDLYFAALSGSAWGQV
                     PISTPEELVERISPADAALRSLVLAPVMHGAAQWGMLITFFGGGHVTLSTARGFDAHA
                     SWRLAETERCMTLLVVGDAMARPLAEALDDGAADYDLSSVIAIGSGGAVFSAPVKNQL
                     KAHLPNLFITDSFGATELGAAGSQMDPDAGARFPHNEFMSVLDEHLEPCPVGVVGRLA
                     RRGNIPLGYWGDEEKTAATFMTDARGVRWAVPGDFCRLEDDDTITLLGRGSGCINSGG
                     EKIFPEEVEVALKSHPGVFDVLVVGVPDERFTERVAAVVQPRPGHVVTLDDLADHCRT
                     RLARYKAPRELRLVDEIGRTASGKADYRWAKAQFE"
     gene            14197..15111
                     /locus_tag="PKY13_03415"
     CDS             14197..15111
                     /locus_tag="PKY13_03415"
                     /inference="COORDINATES: protein motif:HMM:NF025697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HOT79006.1"
                     /translation="MPRTPQRLAGVTATALAAIVLASCSPGPGKFIPGECGRNRVVRT
                     DIVALTDTGQLACIEGADTDATKVIGTLTGLESNETLVGIDYRSPFKDAGGVSNNVPV
                     EGTLYGLGNLGGLYSINPSTAVATKKAQISVALAGTAFGIDFNPTVDRLRVVSNTGQN
                     LRVNVDTGAALVDGGLVSGGTTATGVAAAAYTNVDSDPATGTTLFDIDVNSDQLLVQN
                     PPNDGVLVGIGAPLGIDATGPVGFDLYSSVKDIGGGVLTTTDLAGYATVATAAGRSLY
                     QVTPFSGRLVNLGDLGLPVRDIAIPLAQ"
     gene            complement(15131..16144)
                     /locus_tag="PKY13_03420"
     CDS             complement(15131..16144)
                     /locus_tag="PKY13_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT79007.1"
                     /translation="MVGRVVPGGPFRVGILAFDGCFAAEIFGLSDLLLMANRVALAST
                     ATVGDQFEVSVLSAGGGPVSAAGGCPLGATLAHTGLDVLVVPGFEVMPGDDLDATLGA
                     WTAETRFIAAAVAVGVPVASVCVGAFLLGEAGVLDGRRATTSWLFSAALASRFPDVSV
                     TPEAIVVADDAVTTTAGFSAVHDLAIRLIRRHAGEQVARRTARVALVADNRTTQTPYV
                     DDSMLLAPSSRFAHDVQRRLLERLDQPYDLGALSREFNVSTRTMLRRFAAEAGRSPLD
                     FLQSARVQAAKRLLETDDMSVAAVMEQVGYRDAGTFRRLFTEQVGVTPAAYRRQFRAV
                     GAR"
     gene            16218..>16471
                     /locus_tag="PKY13_03425"
     CDS             16218..>16471
                     /locus_tag="PKY13_03425"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOT79008.1"
                     /translation="MRLTPSYGPDPVISLDGPPDAVAAPAIRQRRRLVALLASLDEAA
                     WSHPSRCEGWTNRDVVVHLDSTNAFWAHSITEGLHGRPTR"
BASE COUNT         2613 a         5997 c         5467 g         2394 t
ORIGIN      
        1 tgaggggttt gacccgcagg cccgctgcgg agccctcggc caacgtcgcc agccggctgg
       61 cgcgggtctc ctcccgtttg gcgctcgtga cccagtgcgt ggcggccttg cggtaactcg
      121 gagccgacga ttcccagaac tcccacgcac ccggcaccga gcggagcacc gcctcctgct
      181 cgacgctgag agtcgcgggc tcgtcctgct cgtgggagta gatggccgag cggtcctcac
      241 ggcgagcctc gaacgcagca agccccgccg ggtacatgag ccccgcttcg gtgaacgcgg
      301 cggccttggc gatgttgacc gcgctccaga tcgagccggc cttcctcggg gtgatccgct
      361 gggcatagac ctcgtcgtcg atgcccttgc ccacgccgtc gatccacccg aagcacagca
      421 cctgatcgac gatctcgggc cacgtcaccg acggcttgcc actgcccttc ttgtaccgac
      481 cgatccacag ctcggtctcg cgctgatggt gctcgaccag ccaggcccgg aggtcaccgg
      541 gcgtggggaa gaacacgagg ttccggggct cgactgccat cgagtcacgg taggcgccga
      601 gcgctacccg cggagcacct tctccatcgg ccgccccttg gcgagctcgt cgacgagctt
      661 gtcgaggtag cggatctgcc gcatgagcgg gtcctcgacc tgaagatccg gtgcacctcg
      721 ccatcgtagg atcgtcagag gaccccgcct gagtaatccg tgccggatgc gacgcgtcgt
      781 gtcacatcca gcacggatct gcgccgtcct cggtcacggc tcgatcgacg actcctcggc
      841 gcggacgatc ttggcgccgg cgagggtgcc ctcgtcgcgc cagcgctcga ggtgcgccgc
      901 gtagtccatg agcgggccgg ggaagacggc gctccgggcc gacagcatcg tgcgcgcccc
      961 ttcactgttg ccgtagctcg gggtacaggc caggttgtac cgagccagcg gccccgacgc
     1021 ctgccacagg atgttgagcc actcgtcctc ggcctcgacc gtcgtctcga tcgacgcaat
     1081 gccctcctcc agacagtggt cgatcatcca gacgatgtgc ttggtcgact ccgacaggaa
     1141 atgcacgaag ttcagaccga agccggcctg cacgaagctg accacgaaga agttcgggat
     1201 ctccgccgtg aggatgccgt gaagcgagtg ggtgccgtcg tgccaccgct cgctgagctt
     1261 caccccgcca cggccgaccg gatcgaagcc gaggcggtcg accagcccgg tcgtgatctc
     1321 gaaccccgag gcgaacacca ggaggtcgag cgggtactcc gtgccgtcca cgatcggccc
     1381 ggcgggcgac atctcccgga cgccctggcc atcggtgtcg acgaggtgca cgttgggcag
     1441 gttgaacgac tgcaggtact cgtcgtggaa gcagatccgc ttgcagtgct tgccgtacca
     1501 cggcttcagc ttcgcggccg tctcgggatc ctcgaccacc tcgtcgatcc ggcggcggaa
     1561 gccttccatg atctcgaagt cgatcgcttc gagttcggcg cgctcggcat cgtccgccgg
     1621 ctcccgctgc gtgtcgacct tgaggacctg gccccacccg tcggcgacga ggtcgcggtc
     1681 gggctgttgg ccggtggcga ccgacgtgaa gttgagggtc cgctcgagct gccagcccgg
     1741 cttcagcgac cggaaccact cctcgtcgat ctgctgttgg gcgcgcaccc cgacagcgga
     1801 cggcgtccgc tggaagacgt agacctcctt ggcggaccgg gcgagctgcg gcacgatctg
     1861 gaccgacgtc gccccagtgc cgatgatgcc gacaaccttg tcggcaagcc ggtccatcgg
     1921 ctcggtcggg ctgcccccgg tgtactcgta gtcccagcga gtcgtgtgga acgccttgcc
     1981 ggtgaagtcg tcgatgccgg cgatgcccgg caacttcgcc ttgtgcatca gaccgccggc
     2041 ggccacgaag aactgggtgc ggatgcggtc accgcgttgg gtcgtgatgt cccaccggtg
     2101 cgcgtcgtcg tcccacacca acgactccac ctcggtctgg aagagtgcat ggtcgtagag
     2161 cccgaagtgg cgaccgagca gctgggcgta tccgaagatc tccgaggcgc tcgcgtactt
     2221 ctcggtgggc atgtagcccg tctcctcgag cagcgggagg tagatggtgg aatcgacgtc
     2281 gcacatgcaa cccgggtagc ggttccagta ccaggtgcca ccgaagtcgg ccgccttctc
     2341 gacgatgcgg aagttggtga tgccgcgctt gacgaggttg atcccggcga gcatcccggc
     2401 ccaccccccg ccgaggacga cgacctgcac gtcctcgacc actgggtccc gggtgaaatc
     2461 ggggtcggcc cacgggtccc ggtcgaggtc gtggtcgcgg agggactcgt actgggcgag
     2521 gccttcactc cggagccgct tggcccgctc ggcttcgtag cgctccttgg ccgattcgat
     2581 cgctgacggt tcgggggtgg tcatctggtg cctccggagc aggcgagcgg tgggttctgt
     2641 cgacgaccga agcagtttct gacggtgtgt cagattcggt cgttgctcac cataaagcct
     2701 cgtgaaggcc gcggggaatt cgttccggga agcaaccgtc ccaaacggta cggtgccgcc
     2761 gttcggcgcc ggagcgtcgg gcgaactggg cgaggcagcc accacgcaac aacgcctcct
     2821 cgacgccctc ggccgtcgcc cccgaccgaa tccacctcgg gcggccccag ggcgctcaga
     2881 cgcgttcgat gatgactgcg ggggccatac cgccaccggc acacatcgtg atgagaccga
     2941 actgtccgtc gatgcgctcg agctcgtcga gcaccgtccc gatgagcatg gcacccgtcg
     3001 cgccgatcgg gtgaccgagc gccattgcgc cgccgttcac gttgaccttg tcccgatcga
     3061 gatcgaggtc gcgcatgaac ttctcggcca cgaccgcgaa cgcctcgttg atctcgaaca
     3121 ggtcgatgtc gccgagcgtc aggccggccc gtgccaggac cttgcgggcc gctggaaccg
     3181 gtgcgttgag catgagggtc ggatcgtcgc ccatgttcgc ggtcgccacg atgcgggcgc
     3241 gggcggtgag tccgtgcgcc ttggcgtagc cctcgctcgc caggaggatc gctgcggccc
     3301 cgtcgacgac acccgacgag ttgccggcgt ggtggacatg ggcgatgtcg agatcgggat
     3361 acttctggtt gatcagctca cggaacgtcg ggttgtcgtt gtgccggtag tcggcgatct
     3421 ggggaaagct ggcgggcaac gccgccagcg actcggccgt cgtacccgga cgggggaact
     3481 gctcctggtc gagggccacc gtgccgtcga gattgagcac cgggatcaag ctcttgtcga
     3541 agcggccctc ggcgatcgca cgggcggcgc gggcctgcga ctctgcggca agggcgtcga
     3601 gcgtcccacg atcgatgccc tcgagcgtcg cgatcgcgtc ggcgcacacg ccctgatgcg
     3661 actgcgggtg gatctcctgg aggtgggcgt tgcccgcgcc catcggaagc aggcccttct
     3721 tcggcagtga catcatctcg caaccgccac cgaccacgag gtcctccatg cccgccatga
     3781 tcgcgttggc agcgatgttg gtggcggtga tgcccgagcc gcagaagcgg tcgagggtca
     3841 cgccgctggc ccggacgtcg tagccggcgt cgagggccga catccgaccg aggtcgccgc
     3901 tctgctcgca cacctgcgag ctggtgcccc agatcacgtc gtcgacatcg gaggtgtcga
     3961 agtcgttgcg ctgggcgaga gcctcgagca cggtgcggcc gaggtgctgg gggtgcaggt
     4021 gcgccagagc gcccttgccc tgcttgccga tgccacgagg ggtccggcag gcgtcgatga
     4081 tcagtgcgtc aggcatggcc gacagcatgg cgcatcgacg tactgccgtg acgggcatcg
     4141 tgggaagctg gagccatgac cgaagccgtt gtcgtcggga gcgggccgaa cggactggcc
     4201 gcggccatca cccttgcgga agcggggctg gcggtcaggg tgctcgaagc cgccgaccgg
     4261 cccggtggcg gcgtccgctc gagcgaggcc accctgccgg gcctgatcca cgacgacggc
     4321 tcgggatttc acccgatggg ggtcgcctcg ccgttcttcc aggcacagga cctcgatcgc
     4381 ttcggcctgg agttcctctg gcccgagatc gagctcgccc atccgctcga tgacggccgt
     4441 gccggcctgc tgtggcggga caccgaacgg accgtcgctg cgctcggcat cgacggagcc
     4501 cgatgggacg cgacgatcgg ctgggcgggc cggcgcttcg atcggatcgg gtccgaaatc
     4561 ctccggccca tcgtgcacgt gccccgccac cccgtggtgt tgtcccgctt cggcgccctg
     4621 tcggccctcc cggcgaccgc cttcgctcgc gtgttccaca ccgaggaggc ggccggcctg
     4681 ttcggcggca tcgccgccca tgggttcggg aagctgacga tgccgttctc gtcctcggcc
     4741 ggcatcatgc tcgggggagc cgggcacgcc tacggctggc ccgtcgcgaa gggcggttca
     4801 caggccatca ccgacgccat ggtcgcgaag ctcacagcgt tgggcgtcac ggtcgagacc
     4861 ggggtgacgg tgacatcgtt cgacgagctc ggctcgcccg acgtcgtgat gctcgacacg
     4921 agccccgccg ccgcagcgcg gatcctcggc gatcgactcc cgagccgcgt cgcacgcgcc
     4981 taccggcgct tccgaccggg cccggcggcg tacaagctcg acatcgcgct cgagggccac
     5041 atcccctggt cgaaccccga cgtcggccgc gccggcaccg tgcacgtcgg cggaacgatg
     5101 gccgagatgg ccgccgccga ggccgacacc aacgaggggc ggatgccgga tcggccgttc
     5161 ctgatcctcg gccagcagta cctcgccgac ccccaacgca gcagcggcgg actccacccg
     5221 atctacgtgt acgcgcacgt gcccaacggg tacgaccgcc ccgagggaac cgagctgatc
     5281 cttcgccaga tcgaacggta cgcacccggg ttccgcgacc tcatccgcca cgtgacggtc
     5341 acgacgcccc ggcaactctc ggcagcgaac gcgaacatcg tcggaggcga catcgccggg
     5401 ggagcgtcga gcggtcccca gttgatcttc cgcccgcgca tcgcgctcga tccctacgac
     5461 accacggtcc ccggggtgtt catctgctcg gcgagcacac cgcccggcgc cgccgtccac
     5521 ggcatgggcg ggttcaacgc cgccacccga gcgctccggc aggtccacgg cggctgagca
     5581 acgggccagt ccggcacgac acgcccgacg cggctgacat caccagttca cacggatcgc
     5641 ttgcacctgg tagttcggac tggaagcatc tcgtacgaca ccgcggcata tgacctggtg
     5701 tgaggagcaa cgtgatgcgt cgaacagtga tcgggatgat ggcgatcgcg gcgacgacgt
     5761 cgctcgtggc ctgtgtaccg gagacacctg cgctgccacc ggtcgccggc ggaaccggcg
     5821 gcggcgactc gtacttcccg gcgatgggca acaccgggta cgacgtcgcg cactacgacc
     5881 tcgatctcga ctacgacccg acgacgaagg cgctcgccgc caccgcagtg atcaaggcag
     5941 cgaccgtcgg cgctccgttg tcgtccttca gcctcgacct ccgcgatctg acggcgtcgt
     6001 cggtgacggt cggcgggcgt cccgcagcat tcacccaggg cggcggcgaa ctcatcatca
     6061 ccccggagtc gcccgtcagc ggcacgttcg tgaccaccgt cgtctacggc ggcacgatgg
     6121 gccagcccct cgacgggacg ggagccctgt acggctgggt tgccttcgac gacggtgcgt
     6181 tcgtcgccaa cgagccggaa ggtgccagca cctggtaccc ggtcaacgac gtgccgtccg
     6241 acaaggccac gtaccggttc accatcaccg tgcccgacgg caccacgggc atcggcaacg
     6301 gcgacctcgt gtcgcagacg tcggcgggag gctccacgac gttcgtctgg gtcgccgccg
     6361 atcccatggc ctcgtacctg tcgttggccg ccagcggcaa ctactcgacc accaccgaga
     6421 cgacgccctc cggtctcccg atcgtcaact tcgtcgacgt cgacctgtca cccggtgacc
     6481 aggcagcagc caccgccgtg ctcgcccagc agcccgccat ggtcgactac ttcgacgaca
     6541 tgttcgggcc gtacccgttc tcgtcgttcg gtgccgtcat cgacgatgac gaggacccgg
     6601 gctacgccct cgagaaccag acccggccga tctacgccgg cgtgccgtcg agcagcaccg
     6661 tcgcccacga actggcccac cagtggtacg gcaacaagat cacgccgaag tactggaagg
     6721 acatctggct caacgagagc ttcgccacct acgccgagtg gctgtggggc gagcacaacg
     6781 gcggcaccac cgtcaccgcc cggtacacga ccgtcaagaa tcgcagtgcg gccaacgcgt
     6841 tctggacggt cgtgaccgcc gacccgggcc ccgacgacct gttcgctgcc gccacctacc
     6901 agcgtggggg agcgttcctg cacgcactcc ggctcacgat cggcgacacc cagttcttct
     6961 cgatcctgcg ccagtgggcg gacggcaacg ccaacacgcc ggtgacgacc gccgacttcc
     7021 aggcgctcgt cgagtcggtg tcgggcctcg acctcgacgc ctggttcgac gcctggctgt
     7081 accagccgac caagccggct gcggcctacc tcccgtagcc accggatctc ggcgttcagg
     7141 gccacacctc gccgcagctg ctcgtcgcag gaacgccggc gaaccgggcc gacaagaagt
     7201 cgagcgccgg ctgcacgctc aacacgatgc cgagcgcgtg ttccgtcggc aggtcggtcc
     7261 acgccaccgt cgcacccgcc gcacaccact ctcggagcag cgtcaccgcc tgcgcccgag
     7321 ggatcacctg gtcgacgaga ccgtggagca gcagtaccgg cgccgccggc gctgcccctc
     7381 ccaagcggtt ctcgtcgagt gccgcgatcc actcgggtgt cgtcaacggg ttggccgatc
     7441 cgaggtagtc actcatgtgg tgcccggcga gcgaaccgaa cgtctggatg ccatcgatgc
     7501 cgacgatgca gaagttccgg ttcgcggcga acgtccgccg tcccgcatcg ttgaggtacc
     7561 ggtcgaggtc gagaccggga taggcggcgt catagcccaa cgcggccaac gtgagcagcg
     7621 agaagaacgg gttgccgtcg aggttcaccg ccacggcccg gagatcggcg ggaacgcctc
     7681 cggcggcgac ggcgaccacg ttcaggtcgg gcgcgtagga cgccgcgagc tgcgcagccc
     7741 accccgctga cgtgccacct tgggagtagc cccagaacgc caccggcgtc gacgcgtcga
     7801 gcccggttcc gtcgagccgc tcagcggccc gggcgatgtc gatcaacgcc ctgccctgat
     7861 ctcggccgac ctcataggtg tgcgtgcccg cggtgccgag gccgaccatg tcgctgacag
     7921 cgaccgccca cccgcgcccg agagcttcct ggaagatgcc gctctcgtag tcgaggccgc
     7981 ccgacagcga cttcgagggg gcgcactggt cgccgacccc tcgcgtcccg accccgtagc
     8041 tcacgagcgg ccgcgacccc gcaccggtcc acggcgttgt cggcacgagc acggtgcccg
     8101 acaccacgac tggctcgccg cgacccgtcg tcgaccggta caggagctgc tgggactcga
     8161 cgccggcgac cggtgcgagc gtgaacgggt cgacggtgaa ccgcgccggc cgggacgaca
     8221 ccacgtcgcc gggcagcgac ccggcgtcga cgaggccggg ccggcgaccg gtgatcggac
     8281 caccggcgtc gtagaacgcg tcgagcgccg gctcaccaca cgacgtgagg gtcgccgcca
     8341 gcacgagcac ggcggcggcg tacaaccatc gatgagatat cggcatgctt ccccctcggc
     8401 gtcgtccgac gagcgactgc cccgacggga gtccggtcga cgggtggaag gtagtcgcca
     8461 cgacgatcgg cccgggtcgg gacgtcagga gtccccgttc aaattaggat cgttcgttcg
     8521 agactcgaag ggagcccgat gaccgaggcc aggcacaccg agcgaggcgc tgacgacccg
     8581 atgctgtccg accgcgaact cgaggtcatg cgcctcctcg cgaagggcat gtcgaacgcc
     8641 tcgatcgcgg ccgagctgta catcggcgtc gagaccgtca aatcgcacct gtccggcatc
     8701 taccgaaagc tcgacgtctc gagccgcggc caggcggtcg catggctcaa cgatcgcccg
     8761 gccctcgcca tcgatcccga tgctccgatc gcctcgacca cgacctcgga gatccccggc
     8821 ggtgtccgcg tcgttctcgc cggcgaattc gacctgacca cgatgcagct cctgtccgac
     8881 gcgctcgacg ctgcccgggc ggcgagcacc ggcgcagtgg aactcgacat ggaacacgtg
     8941 gcgttcatcg actcctcggc gctgcgggtc ctcgtcggat tcatcacccg agaacccaag
     9001 atcccggtgc ggatcgtgaa cccgtcgccc tcggtcaccc gggtcgtcga gttcgccggg
     9061 ctgaagggcc ccttcgggct cgccgactga cacgcacccg tcgcaggcag gggccgacac
     9121 cggcctgccg atcatcgtcg tcaccaaggg cggcgaaccc gagcacccgg cgtggtacca
     9181 ggcgagaacg gagcgccgca tcccggtgct cctcgccagc cccgtggagc gagcccggga
     9241 gatcaggccg gcgcgagggc gagcacggcg atgtcgtcgg tgatcgcacc ggcgtgtgcg
     9301 agcgcccgct cgcggatggt tgccacgatc tcggcgggac gacgtcccac cagttcggcg
     9361 cacaactccc ggagacccgc ctcgccgaac agtcggccct ccgcgtcgcg ggcctcggtc
     9421 accccgtcgg tgtagagcac cagcgtgtcg cccggagcga gcccgacgtc ggcgtcgacc
     9481 caccgcccgt cgggaagcac cccgaggagt ggcccgcgca gctcgatgac gtcgacgtcg
     9541 cccgacgccc ggatcaccaa cggcggcggg tggcccgcag cactcagcct gccgctgact
     9601 ccgccggcgt agtcgacgtg cagcatcgcg aacactgcgg tgcagaagcg gccgtcgtgg
     9661 atcgtcgccg ccagcagcga gtcgagcgat tccatcagac cggcgggact ggtcgcccac
     9721 atggccagcg cccggaaggt gtgccgcacc tggccactga ccgcggccgc ctcgggcccg
     9781 ctcccgcaca cgtccccgat ggcgatcgcg acgttgccac ccccgtcgct cagcgggaag
     9841 gcgtcgtaga agtcgccccc gacgctggag tcggcgcccg gctggaacgc agcggcgatc
     9901 tcgaagccgt cgatctcggg aagctccggc gggagcaggg tacgacgaag gacgtccgcc
     9961 acgttgcgct cgcgctcgga gatggcgtcg tacgcgtcct gcaggcgcgt ccgggtgagc
    10021 cagcccccga gcgacgtccc gaggtcaccg agtgtggcga gcagcgacgg ttcggcctcg
    10081 gtcgagctgg tacggaacag ctcgatgacg gcgaccacct tgccggcgtc gacgaggggg
    10141 aacgcgaggc cgcagcgcag acccgcagcg gccgccgccg tggcccgcgg gaagttgtcg
    10201 tcgacggaca cgtcgacgac ccatgctggt gccgcctcgg tccacacccg gcccggcagc
    10261 ccttcaccgg ggacgaacgc cgtcgcactg ctggcgtcgc tcatggccga cgcatcgagg
    10321 ccgggacgga accagacctg ctcgcagcgc agcgacgacc ggtccgcatc gggttcccac
    10381 agcccggcgg cgtcccaccc gaccgtcgtt gccaccacgg cgaggagccg tccgatcgcg
    10441 tcgggggtgc ccgccgaccc gctgaccacg tcgaggacgt ggtggatcag ggtgctgtcg
    10501 cgctcgagat cgacacgttg gcggacgtcg cgcatggtcg ccacgacgta gccgccgcca
    10561 ccgctcgtcg gcagcatcga gaggaggagc tcgacggcga cctcgttgcc gtcggcacgc
    10621 agcgccggca cccgggtcgg ctggccgatc aggcgcggct cgccgccctc gacgaaacgg
    10681 gacatgcccg ccagatgggc actgcggagc cgttcgggca ccagatcgac gagcggacgg
    10741 ccgacgagct cggcgggtga ccagccgagc agcgcctcgg tggccacgtt cgcgaacagg
    10801 atcgatccgt cgagttcggc gaccaccacg acgtcgttga gcgcgtcgac gaccggcacg
    10861 aggccgccgg ggagcgtgac cgtcgccagc gaacgatcca ccaccttcct cgaccccatg
    10921 gcacaccctc ctcccggccc aaactaccgg ccgatcgtgt cgacgacgtg ccgccgatgc
    10981 gggggatgcc caccggcgtg ctagcaagtt ccgatggagc catcacccga gtccgggggc
    11041 gacgagcctc ctccgtccgc ccctgagccc gacaccggcc gctccggtcg tgcacctctg
    11101 gtggtcgcac tggccctgct cctcgtcggt gtgggcgtcg ccgtggcgct cgtcgtcgga
    11161 ggcgacgaca gcgacaacac cgcctcgccg accagcacca ccgccgtgac gaccacgatc
    11221 gcggcctcga ccacctcggg cgcggccacc gccccggctg cggtcgaccc ggtcacgggt
    11281 ctgccgacgg gccgcttcac catcgtcgga cgccagatcg tcgaccccga cggcaacgtg
    11341 ttcgtcccga tgggcgccaa cgtcgccgtc cgccagggcg ggttcgagga gggctacgcg
    11401 ttcaactgga acggcaccgc caccggtcgc gtcgccgacg tgaaggcgtg gggctggaac
    11461 acggtacgag cgaccctcgt ctgcgacctc gacggcaccg gtcccaccgt cgacgagctc
    11521 aacgccggca tcgacgcgtt catcgaggag tacacccgcc agcgggtcgt cgtgatggtc
    11581 gagtgccacg acatcaccgg cgacaacctc ggcccgaccg atcccaaggt gcaggccctg
    11641 ttcccgttct gggaccgcct cgccacgaag tggaaggaca acccgtacgt ctggttcaac
    11701 ttctacaacg aaccccaggg gaccacgacc gctgcgggcg tcgacaactg gctcgacctc
    11761 cagcgcgacg cgctcggtcg cctccgagcc atcgctccga cgagcatctt cgtcgccgac
    11821 atccccggcg ccgcccaggg cctcggcacc ctcaccggcg atgatcccat cacccgactc
    11881 ggcgacggcc agtgcaacgt gctctacggc tggcattcct acggcgccgt cggcaacgac
    11941 gacgagttcg gctcgcccaa cgactgggac aacgaggcgg cgagtctcgc caaccatcgc
    12001 aaggtgttcg agttcctccg cgacaacgac atcccgatgg tcatcggcga ggtcggcgac
    12061 ccattgacgc tcgacgaagg gaacgccggt cagccgatct ggaaccgata cggggcgcgc
    12121 tcggtcatcg cgctcgcccc cgagttcggc gtcggtctgc tctggtggca cggcaccggc
    12181 gacagcggcg tgttcctcac gtactccctg atggccgaac gtcacgaggc gccgtggtca
    12241 gcggcgacga ccggcgaggg gctctccgac ggcggccgcc gcttctggga agcgtcgaag
    12301 aaccagccgg cgcccacccg attcaccggc gatctggccg ccggcaactg cggctgagcg
    12361 gcatcggaga cggcgacgac gtaccgtgat cgccacgacc gacccgaacc cgcgggacgg
    12421 tcgtgacgag gggggtctcg tggacacgac gacggattcg acgacagcgc gacggcacgg
    12481 tcgggcggtg ttccacatcg ccgacctgtt cgaactggtc gccgaggcga ttcccgaccg
    12541 cccggccctc gtgggcgggg accaccgcct cacctacggt gagctcgacg agcggtcgaa
    12601 ccgcctcgcc aaccatctga tcgccaacgg cctcgaaccc ggtgccacca tctcgatcta
    12661 cgcgtggaac cgcactgagt gggtcgagtc cctgttcggt gcgtacaagg cccgggcaat
    12721 cccgatcaac gtcaactacc gctacctcgc cgacgagctg cgctacatcc tcgacgactc
    12781 caagtccgag gcgctggtgt tcgagcgctc gttcgctccc atcgtcgccg ccgtcatcgc
    12841 cgacctgccc cggctgcggc agctcatcgt gctggaggac ggcagcgacc agccgctgga
    12901 cggccttccc ggcgtcgtgc ggtacgagga cgccctcgcc gatgcctcac ccgagcgggt
    12961 tgacatcgag cggtctcccg acgacctcta catcatgtac accggcggga cgacgggcat
    13021 gccgaagggc gtgatgtggc ggaacgagga cctctacttc gctgccctca gcgggagcgc
    13081 gtggggccag gtgccgatct cgacgcccga ggaactggtc gaacgcatct caccggcgga
    13141 tgccgcgctg cgctccctcg tgctcgcccc ggtcatgcac ggcgcagccc agtggggaat
    13201 gctcatcacg ttcttcggcg ggggccacgt gacgctgtcg accgcccgcg gcttcgatgc
    13261 gcacgcctcg tggcggctcg ctgagaccga gcggtgcatg acgctcctcg tcgtcggcga
    13321 cgccatggcg cgccccctgg ccgaggcgct cgacgacggt gccgccgact acgacctgtc
    13381 ctcggtgatc gccatcggtt ccggtggcgc cgtcttctcc gccccggtga agaaccagct
    13441 caaggcccac ctgccgaacc tgttcatcac cgacagcttc ggtgcaaccg aactcggcgc
    13501 cgccggctcg cagatggacc ccgatgccgg tgccaggttc ccgcacaacg agttcatgtc
    13561 cgtcctcgac gaacacctcg agccatgccc cgtcggggtc gtcgggcgcc tcgcccgacg
    13621 cggcaacatc ccgctcggct actggggcga cgaggagaag accgcggcca ccttcatgac
    13681 cgatgcccgc ggggtccggt gggccgtgcc cggcgacttc tgtcgactcg aggacgacga
    13741 cacgatcacc ctgctgggac ggggctcggg ctgcatcaac tccggcggcg agaagatctt
    13801 ccccgaagag gtcgaggtgg cgttgaagag ccacccgggg gtgttcgacg tcctggtggt
    13861 cggggtgccc gacgaacggt tcaccgaacg cgtcgctgcg gtggtgcaac cgcgcccggg
    13921 ccacgtcgtg accctcgacg atctggccga tcactgccgt acccggctcg cccggtacaa
    13981 ggctccccgc gagctccgcc tcgtcgacga gatcggacgc acagcgtcgg gcaaggccga
    14041 ctaccggtgg gccaaggcgc agttcgagta gcgctccggc atcggccccg cccccgacgc
    14101 catctctgcc accgacacaa tctcgacagg atcccccatc cggtcggagc cccgtgacga
    14161 accttccgca tccatccaac ggagagaagg tccctcatgc cccgaacacc ccagcgcctc
    14221 gcaggcgtca ccgccaccgc gctcgcggcc atcgtgctcg cctcgtgcag ccccggcccc
    14281 ggcaagttca tccccggcga atgcggccga aaccgcgtcg tacgtaccga catcgtggcc
    14341 ctcaccgaca ccgggcaact cgcctgcatc gagggcgctg acaccgacgc caccaaggtc
    14401 atcggcaccc tgaccggcct cgagtcgaac gagacgctcg tcggcatcga ctaccgctcg
    14461 ccgttcaagg acgccggagg cgtgtcgaac aacgtgccgg tcgaaggcac gctctacggc
    14521 ctcggcaacc tcggcggcct ctactcgatc aacccgtcga ccgccgtcgc caccaagaag
    14581 gcccagatca gcgtcgccct cgccggcacc gcgttcggga tcgacttcaa cccgaccgtc
    14641 gaccggctcc gcgtggtgag caacaccgga cagaacctcc gcgtgaacgt cgacaccggt
    14701 gccgccctcg tcgacggcgg gctcgtgtcc ggcggcacca ccgccaccgg cgtcgctgcg
    14761 gccgcctaca cgaacgtcga cagcgatccc gccacgggga ccacgctgtt cgacatcgac
    14821 gtcaactccg accagctgct cgtccagaac ccgccgaacg acggcgtgct cgtcggcatc
    14881 ggcgccccgc tcggtatcga cgcgaccggc cccgtcggct tcgacctgta ctcgtcggtc
    14941 aaggacatcg gcggcggcgt cctcacgacc accgacctcg ccggctacgc gaccgtcgcc
    15001 accgcggccg gccgctcgct ctaccaggtg accccgttct ccggacggct cgtgaacctc
    15061 ggggacctcg gcctgccggt tcgggacatc gccatcccgt tggcccagta ggcagcggca
    15121 ggcaggtcgg ctaccgcgca ccgacggcac ggaactgccg tcggtacgcc gccggcgtga
    15181 ccccgacctg ttcggtgaac aatcggcgga acgtcccggc gtcgcggtag ccgacctgtt
    15241 ccatcacggc ggcgacgctc atgtcgtcgg tctcgaggag acgcttggcg gcctgcaccc
    15301 gggccgactg gaggaagtcg agcggcgacc ggccggcctc ggcagcgaac cgccgcagca
    15361 tggtgcgggt gctcacgttg aactcccgtg acagtgcccc gaggtcgtag ggctggtcga
    15421 gacgctcgag gagccgccgc tggacgtcgt gggcgaaacg ggacgagggg gcaagcagca
    15481 tcgagtcgtc gacgtagggc gtctgggttg tgcgattgtc ggcgacgagc gcgacccggg
    15541 cggtccggcg ggccacctgt tcgccggcgt ggcggcggat gagtcgtatc gccaggtcgt
    15601 gcacggcgct gaagccggcg gtcgtcgtca cggcatcgtc ggcgacgacg atcgcctccg
    15661 gcgtcaccga cacgtcgggg aagcggctcg ccagggcggc ggagaacaac cacgacgtcg
    15721 tggcccgacg gccgtcgagc acccccgcct cgccgaggag gaaggccccg acgcagaccg
    15781 acgccacggg cacccctacc gccaccgctg cggcgatgaa ccgcgtctcc gccgtccacg
    15841 ctcccagcgt ggcgtcgaga tcgtcgccgg gcatcacctc gaagccgggc accacgagca
    15901 cgtcgaggcc ggtgtgggcc agcgtcgccc cgagcgggca tccgccagcg gcgctgaccg
    15961 gcccgccccc ggcggacagc acggacacct cgaactggtc gcccaccgtg gcggtcgacg
    16021 ccagcgcgac gcggttggcc atgagcaaca ggtccgacag cccgaagatc tccgcggcga
    16081 agcatccgtc gaacgccaag atgccgacac ggaacggccc acccggaacg actcgtccca
    16141 ccatgtggcg agaatacccc tcgactctgg cgatcctgcc actcgatccg acgacgatcg
    16201 ctgagcacac tcgatgcatg cgactcaccc ccagctacgg ccccgatccc gtcatctcac
    16261 tcgacgggcc gcccgacgcc gtcgccgcgc cggcgatccg ccagcgccga cgcctcgtcg
    16321 ccctactcgc ctcgctcgac gaggccgcgt ggtcacaccc gagtcgctgc gaaggatgga
    16381 cgaaccgcga cgtcgtcgtg cacctcgact ccaccaacgc gttctgggcc cactcgatca
    16441 ccgaaggcct ccacgggcga ccgacacggt t
//
LOCUS       DAOSOA010000028        16178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3626_length_16178_cov_6.621534, whole genome shotgun sequence.
ACCESSION   DAOSOA010000028 DAOSOA010000000
VERSION     DAOSOA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16178
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3626_length_16178_cov_6.621534"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKY13_03430"
     CDS             <1..228
                     /locus_tag="PKY13_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79009.1"
                     /translation="DPGRAIEAPPADPGPPEAGPLDAELTDELLLMARWLDRNAGRIR
                     VNHVEGELSSPVRPVPVPGRVAVPSARRLAS"
     gene            complement(257..1174)
                     /locus_tag="PKY13_03435"
     CDS             complement(257..1174)
                     /locus_tag="PKY13_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79010.1"
                     /translation="MSRFHRTISVAGAATLGLALLAGCSQPGTITISSKRPSATVRDG
                     SAPAGATLAALRSAGEATSSVTSGRYESTIADGDGNVFMTLTGEFAGDRTRQSTRLST
                     GELLGEDAPADGVTAEMITDGSTLYVGGSLLAGITGLFSDSGEGDEAGAGQVEWYRVD
                     VPEGDTSFDGGDLFGSAFGQDSGRATTSLLSGAYGEVTTIGPEEVRGIATTHSSVDVD
                     VSKVLGEVGGLTDELGEGTVPVDVWLGDDGLVHRMRFEISPTALGLPGEGATSMVSTF
                     ELWDVDADITIDVPENATAFDPTTIFGGR"
     gene            complement(1241..2287)
                     /gene="trpD"
                     /locus_tag="PKY13_03440"
     CDS             complement(1241..2287)
                     /gene="trpD"
                     /locus_tag="PKY13_03440"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOT79011.1"
                     /translation="METDGFEGWTKVLGRLAAGADLTSAESRGALAEVLAGGATSAQL
                     AAFIVALRLKGETAEEITGLVEAMVAVAAPLRLPAGAIDIVGTGGSPTLRDRAFNVST
                     VASIVAAAAGATVCKHGNRMASSTSGSTDVLEQLGVAVDLDGAGVERCIEAAGVGFCF
                     ARMFHPAMRHVGPTRSELGIPTVFNILGPLSHPGGVTRQVLGVADPARVDVIAEVLRQ
                     RGMKRAMVVHGHDGLDELTTTDRTLVIDIVDGEVSRREIDPADVGLERRTVDDIAVGD
                     PAANAEVARRVLGGELSAHRDIVVLNAAAGLVVADIAGDLADGVILAAAAIDDGRAAA
                     TLDRLVAASNASND"
     gene            complement(2325..2579)
                     /locus_tag="PKY13_03445"
     CDS             complement(2325..2579)
                     /locus_tag="PKY13_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT79012.1"
                     /translation="MDRNWVGRAACSGLDPAIFYPPTDEEADAAKAICGECPVMEACL
                     EHAIDNREKNGVWGGATERERLRIIRRRRRVRAQVGNIAS"
     gene            2765..4453
                     /locus_tag="PKY13_03450"
     CDS             2765..4453
                     /locus_tag="PKY13_03450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOT79013.1"
                     /translation="MTLAAFERATSLDRLRHETFDLVVIGGGVTGAGVALDAASRGLK
                     VALVERDDFSSGTSSKSSKLIHGGLRYLQQGDVKLVYEALAERQRLLTNAPHLVKLLP
                     FLLPMFGKGGVIPAKIARLLGTAMWGYDLTGGFKVGKLHKRVTKSEALEYMPTLPAKN
                     LVSGYLYFDAAADDSRLVVAICRTAALHHGAVMANRVTVTGLTQGAAGNVNGVTVAAD
                     GDEFTIATNAVVNAAGVWADRVRTLDEGTDPDSMRPAKGVHLTVPHHLVRNTIAAVIP
                     VPKDRRSVFVVPHGEFTYIGTTDTDYDGSIDDPQCTEDDIDYLLSAINANNVNTITRD
                     DIVGTWAGLRPLVKSASSGRTADLSRGHNIHRSPSGVVTITGGKLTTYRHMAADTVDE
                     VVSEVLGARAPRGAKRSVTKKLRLRGADGYQSVTAAARGYGVTNAVADHLANRFGGEA
                     RAVMAMIKDDPTLAEPLVPGLPYVRAEAIFAVRHEMARSVDDVLSRRTRARLYGRADS
                     AAAADAVAELIAPELGWSAAEAAASAAEFRALCEHERDAAGLEDVLLGTALATV"
     assembly_gap    4455..4554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4555..6081
                     /locus_tag="PKY13_03455"
     CDS             <4555..6081
                     /locus_tag="PKY13_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT79014.1"
                     /translation="PMTAAPETGPAQPTPPIALAGGTTTGRLGAARVDVNEATLARLQ
                     SACAGVTTDAVTLAESSRDWWPLAMTWALDGQVGELASVVCRPSTNDEVAAVAAICNE
                     ERIPLTVAAGRSGVGGGSIPVHGGVVLDLCGLSGIIDVDDHSLVARVRAGTFGDHFEH
                     ELRTTYGLTAGHWPQSMTLATVGGWIACRGAGQLSTRYGKIEDIVTGLEVVLADGTTV
                     HTGGYPRQAAGPDLNQVFIGSEGTLGIVVEAELRLHPAPTHERRGAWGFASFADGLEA
                     CRRILRRGATPAVLRLYDTIESDRNYHTGAPGTDGALNVVVALDEGDEPIVEGSWTVV
                     EAECADAVRLSDDLVEHWLGKRNDVSQLEALISGGLVVDTMEISAPWAHLERIYHEAT
                     TAIGGVEGVLAVSAHQSHAYTDGACLYFTFAGKPGATAKDEFYRAIWDAGTRAVLAAG
                     GSLSHHHGVGLNRSRYMAEALGNGLGVIAAIKAGLDPNGILNPGKLGLPDPFGSVVVP
                     "
     gene            6078..6500
                     /locus_tag="PKY13_03460"
     CDS             6078..6500
                     /locus_tag="PKY13_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79015.1"
                     /translation="MIDPDPTPARITLYWSAIWRAAITTVTFAVPLSLLQKWLVESDR
                     VAGDEAVNFLLNLAIMFCGALGGYAAAKLAPTSRLQNGAAAAALGAIVIQVAGAIRHT
                     IIGRDISSPISWIFLALLLSIFGMFGAWVFGKTNGSTT"
     gene            6497..>6828
                     /locus_tag="PKY13_03465"
     CDS             6497..>6828
                     /locus_tag="PKY13_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT79016.1"
                     /translation="MTILVLDIGTSGVRAAIVGPDATILHERSHEFLPDTPFDGLVEF
                     DAVAYADLAIELARDVLTWDGLASHGEVEAIGISNQRASTVVWDRQTGEPVAPAQSWQ
                     DLRTIGDCL"
     assembly_gap    6829..6928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6929..7783
                     /locus_tag="PKY13_03470"
     CDS             <6929..7783
                     /locus_tag="PKY13_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT79017.1"
                     /translation="GRLATATVFDRELPICGLAGDQQASLIGQGAVQPGLAKITFGTG
                     GMLDVCLGPDRPLEASRSDGGTFPIVCWQRDGEQMWGLEAIMLSCGTNVQWLRDDLGI
                     IETAADSEVVAAGCESTDGVMYVPAQLGLGTPHWDYGARGTLLGLTRGSGRSHITRAV
                     LEGIAHRGVDLVEAAEADAGIRIERLRVDGGMTDNTVFVQALADISGRPVEVSPVRDA
                     TAVGAALLAGLEIGTWSSWDDIAATWRPTKVVEPTEGYDRDTQRAQWHSAIDRAKGWY
                     GDLSALNF"
     gene            7826..8210
                     /gene="rnpB"
                     /locus_tag="PKY13_03475"
     ncRNA           7826..8210
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKY13_03475"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            8244..8915
                     /locus_tag="PKY13_03480"
     CDS             8244..8915
                     /locus_tag="PKY13_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79018.1"
                     /translation="MTTIVRWAKVDALDPDLVPEWLTPAERGRHRSLRQPVDRRQFVG
                     TRQLLRDTLIELLGATADNIELHQQCDRCGGAHGRPTVRIAGRPGPHVSTTHAGGLVA
                     VAIASNPVGVDLEPVDGHTDTNTNIGDLVTWVRTEAVLKATGHGLDIDPRLVGISDPD
                     AAPRLEAWNGPGRRPAVTLTDLDLGGGFVGCVARLGRGRAGVDVAERTLAVLSSSSST
                     SEPAR"
     gene            complement(8898..10208)
                     /locus_tag="PKY13_03485"
     CDS             complement(8898..10208)
                     /locus_tag="PKY13_03485"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOT79019.1"
                     /translation="MPASSNGLDPYVPERGELGYDVLHYDLDLKYSINSNRLDGRARI
                     TIRPTDTLRRVSFDLAGLRVTKATVGGRAARWQQRAEKVRVEPTVSLPAGRTVDVEFR
                     YQGTPGPTSSVWGPVGWEELTDGVLVASQPSGASTWFPCNDLARQKASYRVAVTTSSA
                     YRVVVNGSLRSRRSSGSTTTWVYEQPEPTSPYLMAVNIGRYGEFEFEGSDPAIDAVFA
                     PEHRADFDVAFARLPEMMRLFEDRFGPYPFEAPFTVVLCAEPLEIPIEAQGQAMFGTN
                     HLSPRHHRLIPHELAHQWFGNSVTAANWRDVWLHEGFACYAEWIWSEASGGPSAEEMA
                     STHLRRLRTLPEDLVVGDPGAADMFDDRVYKRGALAVHGVRGVVGDDAFFDLLRRWTR
                     IHRHGIVTSRDFEDLAAATAGRSLERLFDAWLREAALPASTSRF"
     gene            complement(10217..14101)
                     /locus_tag="PKY13_03490"
     CDS             complement(10217..14101)
                     /locus_tag="PKY13_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOT79020.1"
                     /translation="MSEVLADPLRGGPFAPPERTLVDVLIATAQRFPEEPALDDGIGV
                     LSYRDLLAHVDALAEQLVGHGVGHGSRVGIRMSSGTNDLYIAVLATLWAGAAYVPVDA
                     DDPEERISLVLGEAAVTVVVTDGPTITPTGTGTDPADGAERRPVAPDDDAWIIFTSGS
                     TGVPKGVAVTHRSAAAFVDAEAHLFLAGELDDPLGPGDRVLAGLSVAFDASCEEMWLA
                     WRYGGCLVPAPRSLVRSGMDLGPWLVNHGITVVSTVPTLVGLWPADALDHVRLLIFGG
                     EACPPALAERVAVGGREVWNTYGPTEATVVACAAPLTADGPVRIGLPLAGWDLAVVDE
                     AGNEVEPGATGELVIGGVGLARYLDAERDAERFAPMESLGWDRAYRSGDLVVRDPEGL
                     LFVGRADDQVKLGGRRIELGEVDAALLELPGVTSAAAVVQRTGVGHQVLVGYLVGDDL
                     DLRAATESLRRSLPAALVPLLTVCDEIPVRTSGKVDRAALPWPLVGRDDEADAALHGS
                     VGWLAERWADVLGVRVGSLDEDFFELGGGSLAAAQLVSVLREEYPQATVADIYGFPRL
                     AAMADRMEELRAIGQPEGRAWVRRTPRPTQGLQLVLTLALQTVVGLRWLVWTLTLTGA
                     LARFADVAWVPTVSWWWVGVGWLALISPFGRMALAATGARLLLRGVGPGTYPRGGSVH
                     VRIWAVEQLAEAAGAANLAGAPWISYYARALGATVGKGVDLHTLPPVTGLLTLGDGAS
                     VEPEVDLAGYWLDGDELRVGRVTIGAGATIGSRTTLLPGTEIGRDAEVLAGSAVSGRV
                     PARERWSGSPARRVSKAKPPWPEERPPRRPIWVLAYGAASGAMALLPLIAAAVGGAVL
                     GWAVRDADDLGSALVRALVAVPVATVAGLVTLALLTLAGVRLLGIGVREGTFAVRSRI
                     GWQVWATERLLDAARTQLFPMYASLITPWWLRALGAKIGSGVEASTVLLLPKMTTVGD
                     GAFLADDTMIASYELGGGWLRIDHAKVGKRAFLGNSGMAAGGRSVPKNGLVAVLSAAP
                     KKSKAGTSWLGSPPVKLRRTVVDGDDERTFAPPTRLRVARGAVELCRLVPVMVSVAIA
                     VGVVATLVQLADRVGMVATALVSGAVMLAAGFVAAAVTTLAKWAIVGRIAEDEHPLWS
                     SFVWRNEVVDTFVEMVAAPWFANAAMGTPALSMWLRSLGASVGRGVWCESYWLPEADL
                     VKLGDGVSVNRGCVLQTHLFHDRVMSTGTVLLDHGATMGPHGVILPAASVGTDATIGP
                     ASLVMRGEHVPDRTRWAGNPIAPWLGTAGGEE"
     gene            14380..14604
                     /locus_tag="PKY13_03495"
     CDS             14380..14604
                     /locus_tag="PKY13_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79021.1"
                     /translation="MTDLPQPDYDEAIDVVVENLAVHYPTIDQAEIRGLVAEAVARIA
                     SDTKAPSFVAVLAEKETRDILDARTATPEI"
     gene            complement(14854..15426)
                     /locus_tag="PKY13_03500"
     CDS             complement(14854..15426)
                     /locus_tag="PKY13_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79022.1"
                     /translation="MTWTLAMSVGACGADREVDVVAGDSAVTDVAIPVRDTGDTDLCA
                     RIRSGEEEAAKAVREERPPRSRDRLSDPVICAILSNNLPVAQVLVDLDYDLNWRIPED
                     PPVMSVFNVGDVDSIAPTFDGGTWTERRRAVLRLFLDNGLDACERYSTGTAAGETAYE
                     VAKSAGELNTVRLYEDEGADCSGSRATGGS"
     gene            complement(15456..>16178)
                     /locus_tag="PKY13_03505"
     CDS             complement(15456..>16178)
                     /locus_tag="PKY13_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79023.1"
                     /translation="HPRTSTLSAPTPSGVQNISGPKSPIVPHDLSTYPSAARCRPDQP
                     KYPSPGTTSPTTTILPAKLPCPFGNTPGFWDRLEQMGRIAAGSFECTLNSPLSLEQCQ
                     RVMSIAIDSENQGDYYASFWSREFGTITASEQDAIRNAISHALFIGRVALQFGDQIAW
                     DLGLRTELWEGVLGSACKFGSTDTTVDLQNNLIGIGFSQQIRREHGGDQQLVERIIDS
                     YATLRRTTNGCNGDIINIGAQF"
BASE COUNT         2514 a         5900 c         5317 g         2247 t
ORIGIN      
        1 gatccgggcc gggcgatcga agcgccacct gccgaccccg ggccgcccga ggccggcccg
       61 ctcgacgcag agctcaccga cgagctgttg ctgatggccc gctggctcga ccgcaatgcc
      121 ggccggatcc gggtcaacca cgtcgagggc gaactgtcct cgccggtgcg cccggtgccc
      181 gtgccggggc gggtcgcggt gccgtcggct cggcgcctgg ccagctgagc cgcggcgggt
      241 caggtccgct cgggcctcag cgcccgccga agatcgtggt cggatcgaag gccgtggcgt
      301 tctccggcac gtcgatcgtg atgtcggcgt cgacgtccca cagctcgaac gtcgacacca
      361 tcgaggtcgc accttccccg gggagcccga gcgccgtggg gctgatctcg aagcgcatcc
      421 ggtgcaccag gccgtcgtcg cccaaccaga cgtcgacggg gacggtgccc tctcccagct
      481 cgtcggtcag cccgccgacc tcacccagga ccttgctcac gtcgacatcg acgctggagt
      541 gcgtcgtcgc gataccgcgc acctcctccg gtccgatcgt cgtgacctcg ccgtaggccc
      601 ccgacagcag cgacgtcgtg gcccggccgg aatcctggcc gaaggcgctg ccgaagaggt
      661 cgccgccgtc gaacgaggtg tcaccctcgg gcacgtcgac gcggtaccac tcgacctgcc
      721 cggctcccgc ctcgtcgccc tccccactgt cggagaacag accggtgatt cccgcaagca
      781 acgacccgcc gacgtagagc gtcgagccgt cggtgatcat ctcggcggtc acgccgtcgg
      841 cgggtgcgtc ctcgccgagg agctccccgg tcgagaggcg ggtcgactgg cgggtccggt
      901 cgccggcgaa ctcgccggtc agcgtcatga acacgttgcc gtcaccgtcg gcgatcgtgc
      961 tctcgtagcg gcccgacgtg accgagctgg tggcttcgcc ggccgaacgc aacgccgcga
     1021 gcgtcgctcc cgccggagca ctgccgtcgc gaacggtcgc cgacgggcgc ttcgagctga
     1081 tcgtgatcgt gcccggctgg ctgcaaccgg cgaggagcgc gaggccgagc gttgccgcgc
     1141 cggcgacgga gatggtgcga tgaaagcggc tcatggagcg gagggtaccg gcgcgcccca
     1201 ccgaacggtc cgcagcccgt gtgaaggttt cgtgggccgt tcagtcgttg ctggcgttgc
     1261 tggcggccac gaggcgatcg agcgtcgcag cggcgcgacc gtcgtcgatg gcagccgcgg
     1321 ccaggatcac accatcggcg agatcgccgg cgatgtcggc caccacgagg cccgcggcgg
     1381 cgttgagcac cacgatgtca cgatgggcgc tgagctcgcc gccgagcacc cggcgggcga
     1441 cctcggcgtt ggccgcagga tcgccgacgg cgatgtcgtc gacggtgcgg cgctccagac
     1501 cgacatcggc agggtcgatc tcccgccgcg agacctcacc gtcgacgatg tcgatgacga
     1561 gcgtgcgatc ggtggtggtg agctcgtcga gcccgtcgtg accgtgcacg accatggccc
     1621 gcttcatgcc ccgctggcgg agcacctcgg cgatgacgtc gacccgcgcc gggtcggcca
     1681 cgccgagcac ctgacgcgtc acgccaccgg gatgcgacag cggacccaag atgttgaaca
     1741 ccgtcgggat accgagctcg gagcgcgtgg gcccgacatg acgcatggcg ggatggaaca
     1801 tgcgggcaaa gcagaagccg accccggccg cttcgatgca ccgttccacg ccggccccgt
     1861 cgaggtcgac ggccaccccg agctgctcga ggacgtcggt cgagcccgac gtcgacgacg
     1921 ccatccggtt gccgtgcttg cacaccgtcg cgcccgcggc cgcggccacg atgctggcga
     1981 cggtcgacac gttgaaggca cggtcgcgca gcgtgggcga cccaccggtg ccgacgatgt
     2041 cgatcgctcc ggccggcagg cgcagcggtg cggccacggc caccatcgcc tcgaccaggc
     2101 cggtgatctc ctcggcggtc tcgcccttga gtcgcaatgc gacgatgaac gctgcgagct
     2161 gggcggacgt ggcgcccccg gcgagcacct ccgccaaggc cccgcgcgat tcggccgacg
     2221 tgagatcggc gcccgcggcg agccgtccga gcaccttcgt ccagccctcg aacccgtcag
     2281 tctccaacgt cagtcctcac cccagcccac ggcggttccg ccggtcagct ggcgatgtta
     2341 ccgacctggg cgcggacccg gcggcgacgg cggatgatgc ggagccgttc ccgctcggtc
     2401 gccccacccc agacaccgtt cttctcccgg ttgtcgatgg catgctcgag gcatgcctcc
     2461 atcaccgggc actcgccgca gatcgccttg gcggcgtcgg cctcttcgtc ggttgggggg
     2521 taaaagatgg cgggatccaa accgctgcac gcggcacgcc cgacccagtt cctgtccatt
     2581 gactttcgat ccttgcgggc cgttcggtgc cctgtccaat cagttgtggc gggcagttcc
     2641 caccgtgcgg ttgcaacgct ttcaccccat gttttcggtc gtgaattacg tcacatcggc
     2701 tgctgtgcca ggcggcacgg gcccgacttc ggaccgcgtt gttaccccgc ggtaacatca
     2761 gagcgtgact ctcgcagcat tcgaacgcgc cacgtccctc gatcgcctcc gccacgagac
     2821 cttcgacctc gttgtcatcg gcggtggcgt caccggggcc ggtgtcgcgc tcgatgccgc
     2881 gtcgcgtggt ctcaaggttg cgctcgtcga gcgcgacgac ttctcctcgg gcacgtcgtc
     2941 caagtcgtcc aagctcattc acggcgggct ccgctaccta cagcaaggtg acgtcaagct
     3001 cgtctacgaa gccctcgccg agcgtcagcg cctcctcacc aatgcgccgc acctcgtgaa
     3061 gctcctcccc ttcctcctgc ccatgttcgg caagggcggc gtgatccccg ccaagatcgc
     3121 ccgcctgctc ggcaccgcca tgtggggcta cgacctcacc ggcgggttca aggtcggcaa
     3181 gctccacaag cgggtcacca agtccgaggc gctcgagtac atgccgacgc tgcccgccaa
     3241 gaacctggtc agcgggtacc tctacttcga cgccgcagcc gacgactccc gtctcgtcgt
     3301 cgccatctgc cgcaccgcag cgctgcacca cggcgcggtg atggccaacc gggtcacggt
     3361 gaccggcctc acccagggag ccgccggcaa cgtcaacggg gtcaccgtcg ccgccgacgg
     3421 tgacgagttc accatcgcca ccaacgccgt cgtcaacgcc gccggcgtct gggccgatcg
     3481 ggtccgcacc ctcgacgagg gcaccgaccc cgactcgatg cggccggcca agggggtgca
     3541 cctcacggtc ccccaccacc tcgtgcgcaa caccatcgcc gcggtcatcc cggtccccaa
     3601 ggaccgccgc tccgtgttcg tcgtgcccca cggcgagttc acctacatcg gcaccaccga
     3661 caccgactac gacggcagca tcgacgatcc gcagtgcacc gaggacgaca tcgactacct
     3721 cctgtcggcc atcaacgcca acaacgtcaa cacgatcacc cgtgacgaca tcgtcggcac
     3781 ctgggccggc ctgcgtccgc tcgtgaagtc cgcgtcgtcg ggccggaccg ccgatctctc
     3841 ccgtggccac aacatccacc gctccccgag cggcgtcgtc acgatcacgg gcggcaagct
     3901 cacgacctac cgccacatgg ccgccgacac cgtcgacgag gtcgtctccg aggtcctggg
     3961 tgcccgcgcc ccacggggcg ccaagcggtc ggtcacgaag aagctccgtc tccgcggcgc
     4021 cgacggctac cagtcggtca cggcggccgc ccgcggctac ggcgtcacca acgccgtcgc
     4081 cgaccacctc gccaaccgct tcggcggcga ggcccgcgcc gtcatggcga tgatcaaaga
     4141 cgacccgacg ctggccgagc ccctcgtccc gggccttccc tacgtgcggg ccgaggcgat
     4201 cttcgcggtg cgccacgaga tggcccgctc cgtcgacgac gtgctcagcc gccgcacccg
     4261 cgcccgcctc tacggccgcg ccgactccgc ggccgccgcg gacgccgtcg ccgagctgat
     4321 cgctcccgag ttggggtgga gcgcggcgga ggccgccgcc tccgccgccg agttccgggc
     4381 gctgtgcgaa cacgagcgcg acgccgccgg cctcgaggac gtcctcctcg gcaccgcact
     4441 cgccaccgtc tgacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgatg
     4561 actgcagctc ctgagaccgg ccccgcccaa ccgaccccgc cgatcgccct cgccggtggc
     4621 accacgaccg gccggctggg cgccgcccgc gtcgacgtga acgaggccac gctcgcccgc
     4681 ctccagtccg cgtgcgccgg ggtcacgacc gacgcggtca cactcgccga gagcagccgc
     4741 gactggtggc cactcgccat gacgtgggcg ctcgacggcc aggtcggcga actcgcgtcg
     4801 gtcgtgtgcc gccccagcac caacgacgag gtcgcggcgg tcgcggcgat ctgcaacgag
     4861 gagcgcattc ccctgacggt ggccgccggc cgctccggcg tcggcggtgg atccatcccg
     4921 gtccacggtg gcgtggtgct cgatctctgc ggcctgagcg gcatcatcga cgtcgacgac
     4981 cactccctcg tcgcccgggt ccgcgcgggc acgttcggcg accacttcga gcacgagctc
     5041 cgcaccacct acggcctcac cgccggccac tggccgcagt cgatgacact cgccaccgtc
     5101 ggcggatgga tcgcctgccg cggcgccggc cagctgtcga cgcgctacgg caagatcgag
     5161 gacatcgtga cggggctcga ggtggtcctc gccgacggca ccaccgtcca caccggcggc
     5221 tacccccgcc aggcggccgg tcccgacctc aaccaggtgt tcatcgggag cgagggcacc
     5281 ctcggcatcg tcgtcgaggc cgagttgcgg ctccacccgg cgccgaccca cgagcgtcgg
     5341 ggggcgtggg gcttcgcgag cttcgccgac gggctcgagg cctgccgccg catcctccgt
     5401 cgaggggcga cgcccgccgt gctccggctg tacgacacca tcgaatccga ccggaactac
     5461 cacacgggcg ctccgggaac cgacggagcg ctcaacgtcg tggtcgcgct ggacgagggc
     5521 gacgagccga tcgtcgaggg cagctggacg gtggtcgaag cggagtgcgc cgacgccgtg
     5581 cgtctgagcg acgacctggt cgagcactgg ctcggcaagc gcaacgacgt ctctcagctc
     5641 gaggcgctca tcagcggcgg gctggtcgtc gacaccatgg agatctcggc gccgtgggcc
     5701 cacctcgaac gcatctacca cgaggcgacg accgccatcg gcggcgtcga aggggtcctg
     5761 gccgtctcgg cgcaccagtc ccacgcctac accgacggcg cctgcctgta cttcacgttc
     5821 gccggcaagc cgggggcgac ggccaaggac gagttctacc gggcgatctg ggacgccggc
     5881 acccgcgccg tcctcgctgc gggcggctcg ctcagccatc accacggcgt ggggctcaac
     5941 cgctcgcggt acatggccga ggcgctcggg aacggcctcg gcgtcatcgc cgccatcaag
     6001 gccggcctcg atcccaacgg catcctcaac cccggcaagc tcggcctgcc cgatccgttc
     6061 ggcagcgtcg tcgttccgtg atcgaccccg atccgactcc ggcgcgcatc acgctctact
     6121 ggtcggcgat ctggcgggcc gcgatcacga cggtcacgtt cgccgtgccg ctctcgctcc
     6181 tgcagaagtg gctggtcgag agcgaccgcg ttgccggcga cgaggccgtc aacttcctcc
     6241 tgaacctggc gatcatgttc tgcggcgcgc tcggcgggta cgccgccgcc aagctggcgc
     6301 cgacgtcacg cctacagaac ggtgcggccg cggcggccct cggcgccatc gtcatccagg
     6361 tggccggagc gatccgccac accatcatcg gccgggacat ctcgagcccg atcagctgga
     6421 tcttcctggc gctgttgctg tcgatcttcg gcatgttcgg cgcgtgggtg ttcggcaaga
     6481 ccaacgggag cacgacatga ccatcctcgt cctcgacatc ggcacgagcg gcgtgcgcgc
     6541 cgcgatcgtc ggccccgacg ccacgatcct gcacgagcgg tcccacgagt tcctgcccga
     6601 cacgcccttc gacggcctcg tggagttcga cgccgtcgcg tacgcggacc tcgcgatcga
     6661 gctcgctcgc gacgtgctca cctgggacgg cctcgcctcc cacggcgagg tcgaggcgat
     6721 cggcatctcc aaccagcggg cgtcgaccgt cgtgtgggat cggcagaccg gcgagccggt
     6781 cgccccggcg cagagctggc aggacctccg caccatcggc gactgcctnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ccggctcgcg accgccaccg tgttcgaccg
     6961 cgagctcccg atctgcggcc tcgccggcga ccaacaggcg tcgctcatcg ggcagggcgc
     7021 cgtacagccc ggcctcgcga agatcacgtt cggcaccggc ggcatgctcg acgtctgcct
     7081 cggccccgat cgtccgctcg aggcgtcgcg gtccgacggc ggcacgttcc ccattgtgtg
     7141 ctggcagcgc gacggtgagc agatgtgggg tctcgaggcg atcatgctgt cgtgcggcac
     7201 gaacgtgcag tggttgcgcg acgacctggg catcatcgag acggcggccg acagcgaggt
     7261 ggtcgccgcc ggttgcgagt cgaccgatgg cgtcatgtac gtgccggccc agctcggcct
     7321 cggcacgccc cactgggact acggcgcccg cggcacactc ctcgggctga cccgcggcag
     7381 cggtcgcagc cacatcacca gggcggtcct cgaaggcatc gcccaccgcg gtgtcgacct
     7441 cgtggaagcg gccgaagcgg acgcggggat ccgcatcgaa cggctccggg tcgacggcgg
     7501 catgaccgac aacacggtct tcgtgcaggc gctggccgac atcagcgggc gtcccgtcga
     7561 ggtctcgccg gtccgcgacg ccacggcggt gggcgcagcg ctcctcgccg gactcgagat
     7621 cgggacgtgg tcgtcgtggg acgacatcgc cgcgacgtgg cgcccgacca aggtcgtcga
     7681 accgaccgag gggtacgacc gcgacaccca gcgcgcccaa tggcactcgg ccatcgaccg
     7741 cgccaagggc tggtacggcg acctctccgc gctcaacttc taacgccccc gggccctgct
     7801 cgggcgcgta cactcgatcc gctggtgagt ccgttgggtg accgcgggcc gggcgccgcg
     7861 aggcatccga cctgaggaaa gtcggggctc cacagggcag gaagctggcg agagccaggt
     7921 gggggcgacc tcacggacag tgcaacagag agcagaccgc cgatggctcc cccggttctc
     7981 cgggagagca caggtgaggg tgaaacggtg cggtaagagc gcaccagcgt cacgagtgat
     8041 cgtggcggct cggtaaaccc cttccggagc aaggccaagt cgtgggatgg gctgcccgtc
     8101 cgacacctcc gggtgtcacc cacaggtggg ctgcacagat ggatggtcac cgaaccggac
     8161 cgccgcgagg caccggggaa caggaccccg cttacaggcg ggctcaccag cacccaccga
     8221 ccacgccgcc taccgtggtc atcgtgacga cgatcgtgcg atgggcgaag gtcgacgccc
     8281 tcgaccccga cctcgtgccg gagtggctca caccggcaga gcgggggcgt caccggtcgt
     8341 tgcgccaacc ggtcgatcgg cgtcagttcg tcggcacccg acaactgctc cgcgacaccc
     8401 tgatcgagct gctgggcgcc accgctgaca acatcgaact ccaccagcag tgtgatcgct
     8461 gtggcggcgc tcacgggcga ccgacggtcc gcatcgccgg tcggcccggg ccacacgtca
     8521 gtaccaccca cgccggcggc ctcgtcgccg tcgcgatcgc gtcgaacccc gtcggcgtcg
     8581 atctcgaacc cgtcgacggg cacaccgaca ccaacacgaa catcggcgac ctggtcacct
     8641 gggtccgcac cgaagccgtg ctcaaggcca cggggcacgg gctcgacatc gaccctcggc
     8701 tcgtgggcat cagcgatccc gacgccgcgc cgcggctgga ggcgtggaac ggtcccggcc
     8761 gacgaccggc ggtgaccctg accgatctcg acctcggcgg cggattcgtc ggctgcgtcg
     8821 cccgtctcgg gcggggccgg gccggtgtcg acgtggccga gcgcacgctc gccgtcctgt
     8881 cctcctcgag ctcgacgtca gaaccggctc gttgacgccg ggagcgccgc ctcgcggagc
     8941 cacgcgtcga acaaccgctc gagcgaacga cccgcagtcg cggcggcgag gtcctcgaag
     9001 tcgcgggacg tgacgatgcc gtgtcggtgg attcgggtcc agcggcgcag caggtcgaag
     9061 aacgcgtcgt cgccgacaac accacgcacc ccgtgcaccg ccaacgcacc ccgtttgtag
     9121 acccggtcgt cgaacatgtc ggccgcgccg ggatcgccca cgacgaggtc ctcgggcagc
     9181 gtgcgcagcc gtcgaaggtg cgtcgatgcc atctcctcgg cggacggacc tcccgacgcc
     9241 tccgaccaga tccactcggc gtaacaggcg aacccctcgt gcaaccacac atcacgccag
     9301 ttggccgcgg tcacgctgtt gccgaaccac tgatgggcga gctcgtgggg gatcaatcgg
     9361 tggtgccgtg gcgacaggtg gttcgtgccg aacatcgcct gtccctgcgc ctcgatgggg
     9421 atctcgagcg gttcggcgca gaggacgacc gtgaacgggg cttcgaacgg gtacggcccg
     9481 aagcggtcct cgaaaaggcg catcatctcc gggagccggg cgaacgcgac gtcgaagtcg
     9541 gcacggtgct cgggggcgaa cacggcgtcg atcgccgggt ccgagccctc gaactcgaac
     9601 tcgccgtacc gcccgatgtt caccgccatg aggtacggcg acgtgggctc cggctgctcg
     9661 tacacccacg tggtggtgct cccgctcgac cgccgggatc gcaacgatcc gttgaccacg
     9721 acgcggtatg ccgacgacgt cgtcaccgcc acccggtacg acgccttctg gcgggcgagg
     9781 tcgttgcacg ggaaccacgt cgacgctccg ctcggttgcg acgcgacgag cacaccgtcg
     9841 gtgagttcct cccacccgac cggcccccac accgacgacg tcggaccggg cgtcccctga
     9901 tagcggaact cgacgtcgac ggttcggccg gccgggagcg acaccgtcgg ctccactcgt
     9961 accttctcgg cgcgctgctg ccagcgggcc gcacgaccgc cgacggtcgc cttcgtcacc
    10021 cggagaccgg ccaggtcgaa cgagacgcga cgcaacgtgt cggtcgggcg aatcgtgatc
    10081 cgggcacggc cgtcgaggcg gttggagttg atcgagtact tcaggtcgag gtcgtagtgg
    10141 aggacgtcgt agccgagctc gccgcgctcg ggaacgtagg ggtcgagacc gttggatgac
    10201 gccggcatcg ccgaggtcac tcctcacccc cggcggtgcc gagccacggt gcgatcgggt
    10261 tgccggccca gcgggtgcgg tcggggacgt gctcgcctcg catgaccagc gacgccggcc
    10321 cgatcgttgc atcggtgccg accgacgcag cggggaggat cacaccgtgg gggcccatcg
    10381 tcgcgccatg gtcgagcagc acggtgcccg tgctcatcac ccgatcgtgg aagaggtggg
    10441 tctgcaggac gcagccccgg ttgacgctca cgccgtcgcc gagcttcacg agatcggctt
    10501 cgggcagcca gtacgactcg caccacaccc cgcggccaac gctcgccccg aggctgcgca
    10561 gccacatcga gagggcgggt gtgcccatgg cggcattggc gaaccacggc gccgccacca
    10621 tctcgacgaa cgtgtcgacc acctcgttgc gccacacgaa cgagctccac agcgggtgct
    10681 cgtcctcggc gatgcgaccg acgatcgccc acttcgcgag ggtcgtgacc gccgctgcga
    10741 cgaacccggc cgccagcatc accgcgcccg acacgagggc cgtcgcgacc atgccgactc
    10801 ggtcggccag ctgcacgagc gtcgcgacga cgccgacggc gatcgccacc gagaccatga
    10861 ccgggacgag ccgacacagc tcgacggcgc cacgggcgac ccgcagacgc gtcggcgggg
    10921 cgaacgtgcg ctcgtcgtca ccgtcgacca cggtccgccg gagcttgacc ggcggactcc
    10981 cgagccacga cgtgccggcc ttggacttct tgggtgctgc ggacaggacg gccaccagcc
    11041 cattcttggg cacggagcgg ccgcccgcgg ccatccccga atttccgagg aacgcccgct
    11101 tgccgacctt ggcgtggtcg atgcgcagcc aaccgccacc gagctcgtag ctggcgatca
    11161 tcgtgtcgtc cgcgaggaac gccccgtcgc cgaccgtggt catcttcggg agcagcagca
    11221 ccgtcgacgc ctcgacgccc gaaccgatct tcgctccgag cgcccggagc caccacggtg
    11281 tgatcaagct ggcgtacatc gggaagagct gggtccgcgc cgcgtcgagg agacgctcgg
    11341 ttgcccacac ctgccaaccg atgcggctcc gcaccgcgaa cgttccctcg cgcacaccga
    11401 tgccgagcag ccgcaccccc gccaacgtca ggagcgcgag cgtgacgagc cccgcaaccg
    11461 tcgcgacggg caccgcgacc agcgcccgga cgagcgcgga gccgagatcg tcggcgtcgc
    11521 ggacggccca gccgaggacg gcgccgccga ccgccgcagc gatcaacggg aggagcgcca
    11581 tcgcacccga cgccgctccg taggcgagga cccagatcgg acgccgcggt gggcgctcct
    11641 cgggccacgg cggcttcgcc ttgctgaccc gccgcgccgg cgagcccgac cagcgctcac
    11701 gagccgggac ccgaccgctg acggccgagc cggcgagcac ctcggcgtcg cggccgatct
    11761 cggtgccggg gagcagggtc gtgcgggagc cgatcgtcgc gcccgcgccg atcgtgacgc
    11821 gcccgacgcg gagttcgtcg ccgtcgagcc agtacccggc gagatcgacc tcgggttcga
    11881 ccgacgctcc gtcgccgagc gtcaacaggc cggtgaccgg cggcagggtg tgcaggtcga
    11941 cacccttgcc caccgtcgcg ccgagggccc gtgcgtagta gctgatccac ggtgcgccgg
    12001 cgaggttcgc ggcgcccgcc gcctcggcca gctgctcgac ggcccagatg cgtacgtgca
    12061 cgctgccgcc acgcggatag gtgccgggcc cgacgccgcg gaggagcaac cgcgccccgg
    12121 tcgcggcgag cgccatccgg ccgaagggcg agatcagcgc gagccacccg acgcccaccc
    12181 accaccacga cacggtcggc acccacgcaa cgtcggcgaa ccgggcgagc gctccggtga
    12241 gtgtgagggt ccagaccagc catcggagtc cgacgaccgt ctgcagcgcc agcgtcaaga
    12301 cgagctgcag cccctgcgtc gggcgggggg tccgccgaac ccatgcccgg ccctcgggtt
    12361 ggccgatggc gcgcaactcc tccatccggt ccgccatcgc tgcgagccgg gggaagccgt
    12421 agatgtccgc cacggtcgcc tgggggtact cctcgcgcag gaccgacacg agctgggcag
    12481 cagcgaggct cccgccgccg agctcgaaga agtcctcgtc gaggctcccc acgcgcaccc
    12541 cgagcacgtc ggcccaccgc tcggcgagcc aaccgaccga gccgtgcaac gccgcgtcgg
    12601 cttcgtcgtc gcgcccaacc agcggccacg gcaacgcagc gcggtcgacc ttgccggagg
    12661 tgcgaacggg gatctcgtcg cacaccgtga ggagcggtac cagggcggca gggagcgaac
    12721 ggcgcaacga ctcggtcgcc gcccgcaggt cgaggtcgtc gcctacgagg tagccgacca
    12781 gcacctggtg cccgacaccg gtgcgctgca cgaccgcagc ggcgctcgtc acgcccggaa
    12841 gctccaacag cgcggcgtcg acctcgccga gctcgatgcg acggccgccg agcttcacct
    12901 ggtcgtcggc ccgcccgacg aacaggagcc cctcggggtc gcgcaccacc agatcgccac
    12961 tgcggtaggc gcggtcccac cccagtgact ccatgggggc gaaccgctcg gcatcccgct
    13021 cggcgtcgag gtagcgggcc aggcccactc ccccgatgac cagctcgccg gtggccccgg
    13081 gctccacctc gttgccggcc tcgtcgacga ccgcgaggtc ccagccggcg agcggcaacc
    13141 cgatccgaac cggaccgtcg gccgtcaagg gcgcagcgca cgccacgacc gtcgcctcgg
    13201 tcgggccgta ggtgttccac acctcgcgcc cgcccaccgc gacccgctcc gccagcgccg
    13261 gcgggcacgc ctcgccgccg aagatcagca gtcgaacgtg gtcgagtgcg tcggcgggcc
    13321 acagcccgac cagcgtcggg acggtggaga cgacagtgat gccgtgattg acgagccacg
    13381 gccccaggtc cattcccgaa cggaccagtg accggggtgc gggaacgagg cagccgccgt
    13441 aacgccatgc caaccacatc tcctcacacg atgcgtcgaa cgcgaccgac aatcccgcca
    13501 gcacccggtc gcccggaccg aggggatcgt cgagttcgcc ggccaggaac aggtgcgcct
    13561 cggcgtcgac gaaggcagcg gccgagcgat gggtgacggc cacccccttg ggaacaccgg
    13621 tggagcccga cgtgaagatg atccaggcgt cgtcgtcggg agccacgggg cgccgttcgg
    13681 ccccgtcggc cggatcggtg ccggtcccgg tcggggtgat ggtgggcccg tcggtcacca
    13741 ccacggtcac tgccgcttcg cccaacacga gcgagatccg ctcctcggga tcgtcggcgt
    13801 cgaccgggac gtaggcagcg cccgcccaca acgtcgcgag gaccgcgatg tagaggtcgt
    13861 tcgtgcccga cgacatccgg atcccgacgc gcgacccatg cccaacgccg tgacccacga
    13921 gctgttccgc cagcgcgtcg acgtgcgcga ggagatcgcg gtacgacagg acgccgatgc
    13981 cgtcgtcgag cgccggctcc tcggggaacc gttgcgccgt ggcgatcagg acgtccacca
    14041 gggtccgttc gggcggagca aacggcccgc cgcgcaatgg atcggcaagc acctcgctca
    14101 tggggcgcag cagagcccga cggtcaccgg gccgccggcc ttgctcgacg tcgtcgtctc
    14161 ggacacctgc aacgggctac cagaactcgg gccgtccgtg cccctcgaac gtcgtgatcg
    14221 cactccgcca cctccgggcc cggaacctat ggtcgccgag tgaacagcga acggcgcgca
    14281 gatgacgtca ggatgacgtt ccccccggtc ttggcgacgg attcaccccg cgttcacctg
    14341 agggtgctta cctcacgtca gtccgactca ggagacccca tgaccgacct cccgcaaccc
    14401 gactacgacg aagcgatcga cgtcgtcgtc gagaacctcg ccgtgcacta cccgaccatc
    14461 gaccaggccg agatccgtgg cctcgtcgcc gaggcagtcg ctcgaatcgc cagcgacacg
    14521 aaagcaccgt cgttcgtcgc ggtgctcgcc gagaaggaaa cccgcgacat cctcgacgcc
    14581 aggacggcga cccccgagat ctgatctcgc ccggcgtcgc cacacctgca gaacacgcat
    14641 cgtcgccggt ggtgccgacg tcaccgccgt cgccaggctc atgggccaca atagcatcgc
    14701 cacttcccag ggcacctcgc cgccgctggc cgggcgacac gacacgccgc aacggtcaac
    14761 ccatctggaa ccagatcacc ggcaccgcgt gaacgtcaac tcgtcaggga ccgttctggc
    14821 cgcgcatctt ctcgatcctc actgcggcga gtgtcacgaa ccacctgtag cccgggagcc
    14881 cgagcagtct gcaccttcgt cctcatagag tcgaacggtg ttcaactctc ccgccgactt
    14941 cgccacctcg tacgcggtct ctccggctgc ggtgcccgtg ctgtaccgtt cgcaggcgtc
    15001 aaggccgttg tcgaggaaga gtctgagcac ggctcggcgc cgctcggtcc acgtaccccc
    15061 gtcgaaggtc ggtgcgatgc tgtcgacatc gccaacattg aagactgaca tcactggcgg
    15121 atcctcaggt atgcgccagt tcaggtcgta gtcgaggtcg accaagacct gggctacagg
    15181 caggttgttg ctgaggatcg cgcaaatcac tggatcagag agtcggtccc gagagcgggg
    15241 cggtcgttct tctcggactg ccttagcggc ttcctcctca ccgcttctaa tgcgcgcgca
    15301 gagatccgtg tcgcccgtgt cgcggaccgg aatcgctaca tcggtgaccg cactgtcacc
    15361 agcaacgaca tcgacctcgc gatcggcgcc acaggcaccg accgacatgg ccaacgtcca
    15421 cgtcaccaca acagcgagcg tccgaccccg ccacatcaga actgagcccc gatgttgatg
    15481 atgtcgccat tgcaaccgtt cgtagtacgc cgcaacgtcg cataggaatc gatgattctc
    15541 tcgacgagct gttggtcgcc gccgtgttca cgtcgaatct gctggctgaa tccgataccg
    15601 atgaggttat tctgcaaatc cacagtcgtg tccgtgctgc cgaacttgca ggccgacccg
    15661 agcacgcctt cccacaactc ggtccggagc cctagatccc acgcgatctg atctccgaac
    15721 tgaagtgcca ctcgcccaat gaagagggca tgtgagatcg cgttcctgat ggcgtcctgc
    15781 tcactggcgg tgattgtgcc aaactcgcgt gaccagaatg atgcgtagta gtcgccctgg
    15841 ttctccgagt cgatggcgat agacatcacc ctttggcact gttccaagga gagcggtgag
    15901 ttgagggtgc actcaaagga gccggcggcg attctcccca tctgctcgag gcgatcccaa
    15961 aagcctggcg tgttgccgaa cgggcacggt agcttcgccg ggagaatggt ggttgtcggc
    16021 gaggtcgtgc cgggcgaggg atacttcggc tgatcgggtc ggcatcttgc agcgctcggg
    16081 tacgtcgata ggtcatgcgg cacgatggga gacttggggc ctgagatgtt ttgcacaccg
    16141 gacggtgttg gcgcgctcag cgtcgatgtt ctcgggtg
//
LOCUS       DAOSOA010000029        15509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3824_length_15509_cov_6.501941, whole genome shotgun sequence.
ACCESSION   DAOSOA010000029 DAOSOA010000000
VERSION     DAOSOA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15509
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_15509_cov_6.501941"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKY13_03510"
     CDS             complement(<1..317)
                     /locus_tag="PKY13_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOT79024.1"
                     /translation="MSIFVDENTKVVYQGLTGSQGRYYGLLNRDYGTQVVAGTSPKKA
                     GTDVDGIPVFATIKDAVAETGANASCIFIPAPGVRAAVMEAAEGGIEFIVCITEGVPA
                     QDEA"
     gene            complement(351..1496)
                     /gene="sucC"
                     /locus_tag="PKY13_03515"
     CDS             complement(351..1496)
                     /gene="sucC"
                     /locus_tag="PKY13_03515"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOT79025.1"
                     /translation="MDLFEYQGKQFFAQYGIPVSDGAATDNVDEAVAIADRIGYPVVI
                     KAQVQVGGRGKAGGVKLAANADEAREHATNILGLDIKGHIVKIIWIEVASDIAEEYYA
                     SFTLDRAKKKHLGMLSAEGGVEIEQVAVDNPDAIAKIWIDPVDGLTEAVAREWVAAAK
                     LNPAATEGAVDILLKLYTAYTEGDADLAEINPLILKPTGEVHALDAKVTLDANSTFRH
                     PEYEAYDATQVRDEREHAAHEKGLQYVGLDGSVGVIANGAGLAMSTLDVVSQAGGAAA
                     NFLDIGGGANADVMAGALEVINNDPGVKSIFINIFGGITRGEEVANGIITALGRVTID
                     APIVIRLDGTNAEEGRSILQPHLNDQLQMAPTMLEAARKAVELAAAR"
     gene            complement(1633..2475)
                     /locus_tag="PKY13_03520"
     CDS             complement(1633..2475)
                     /locus_tag="PKY13_03520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT79026.1"
                     /translation="MRYGIVFANTGPLGTAEGAVALAEACDAHGVESVWTVEHVLVPG
                     GYESEYPYSSTGKMPGSEDSPIPDPLIWLTWVAANSSKVRLGTGVLILPQRNPAVLAK
                     EVATLDALSKGRVELGIGVGWLEEEFDALGVPFDRRGQRTDEYIAALREMWTTEETSF
                     AGEFVNWERVMSFPKPHDGSPPIVVGGHSHAAARRAGRLGDGFFPGRQEILGELIETM
                     RTTAESAGRDPDAIEITTGAAMSPEKRQALVELGVSRMVFPPPAFTPDEIGPAIERTL
                     DFLS"
     gene            2568..3071
                     /locus_tag="PKY13_03525"
     CDS             2568..3071
                     /locus_tag="PKY13_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79027.1"
                     /translation="MPIERRSSSAQIRSILIAIVTVVFVATLGYAVFRAATGQKTSVT
                     KGSDGGRWNTGTAQTKADEIAERGPILIPDPAGAQRLPIYISHIGTDPDTGWYVFEAR
                     PPDAPDDCFISWDRDAEEFTAPCVDDTYPADGDGLRNFEATVTKDGDLIIDFTPGPTT
                     TTTSSPE"
     gene            3234..4922
                     /locus_tag="PKY13_03530"
     CDS             3234..4922
                     /locus_tag="PKY13_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT79028.1"
                     /translation="MTLHAHPFSPTDPVVVATNDPATEPAIASVEIRGAGLSKAFGPV
                     QVFDDVSLVAAPGHRLGIVGENGAGKSTLLAILCGTLEADLGDVHRPAGVGYLHQEFP
                     FPPDTDVATVIEAALAPARAIEAELSAAASALGDVDDAGASRRYELALAAAERADVWN
                     ADHRADEVVTGLGLQRVDPGRTIGSLSGGERTRLELATLLIAHPPAMVLDEPTNHLDD
                     EAIDFLTRFLVAFPGPVVTASHDRLFLEQVCTGIVDLDGGRDGAARTYGGSFERYLDL
                     KAKERARWAQQYADEQDQLDRLRHDITHVAPNVAHNAPRTDNDKFIHHFKGGRVNNQI
                     SRRLRNARERLDELEERQVRRPPVALHFEAPTTTGTQSFEPLIGAVGVSVEHRLARVD
                     VAVDAQTRLLVTGANGVGKSTLLAALARRTPTTTGTVTWRKGLRIGYLPQDVVFDDPD
                     LTAERAFDAVARGVTLTDLGLLSGSDLSRPLGQLSVGQRRRVALGCIVANRPHVLVMD
                     EPTNHLSLTLAEELEEALMTTLGAVIVASHDRWLRRRWDGDELRLERAGAGDEN"
     gene            complement(4946..5947)
                     /locus_tag="PKY13_03535"
     CDS             complement(4946..5947)
                     /locus_tag="PKY13_03535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT79029.1"
                     /translation="MSSSPDPFVSVVIPAYNAEPYLATTLRSILDQTFGDFEVIVAEN
                     GSTDATQDVLLEFAERDERVRPLLLGANVGRSAARNAGLDVARGTWVCPMDADDLWFR
                     GRLRELVAASRRYPAVDAFTDDLIEFTPQPDGSVSLGQRYVSRVSWWMGGTHPLRLGP
                     WFRDRECHMRAFIRRDLLERRAIRFPEGLSAGEDLAFYLQVAFAPGSPPPVRVAQANY
                     YYRVGHSTRAANMAESRVRLTEIAIEGTGSEELRRLVEATNPGRMFTYHRADAIWAAQ
                     GRSAERDADVEGVDLVMDRSAGYRQLAINRVFEVVGKLADRHLRPAIAADIAAQLAP"
     gene            complement(5931..>7231)
                     /locus_tag="PKY13_03540"
     CDS             complement(5931..>7231)
                     /locus_tag="PKY13_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT79030.1"
                     /translation="VVLATPNSYEQFLLCLAVSRAGGLPVPVNPQMRPAEIDHVIADS
                     GASFVVKDAGDLDLDPGAPACDPATPATSDVAALFYTSGTTGKPKGVALTHRALVGQS
                     AAAALWPVQLRHDEVVVALPVAHIMGFATLVGLATAALPVYFMRRFRPNDVLNAIESR
                     RCSGFVGVPAMYRMMLEAGADRHDLKSVRVWMSGADVMPSELARSFKKFGASLHVPLV
                     GDLGEALFVEGYGMVEVGGGVAAKVSPPYLPLGVGETVGFRLPGYKFRVVDESDAEVS
                     MGGLGELWVRGPGVLTGYWNAPAATEAAVTEDGWLRTGDLVRVGPLGTVMFCGRRKHT
                     VKSGGYSVYPLEVETLLEEHPDVVEASVVGIPDAKLGEVPAAAVRVRRRAKADGDEIA
                     AWAAERLAAYKAPRRVIVVDELPRTGTEKVQKERLVELFT"
     assembly_gap    7232..7331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7332..7591)
                     /locus_tag="PKY13_03545"
     CDS             complement(<7332..7591)
                     /locus_tag="PKY13_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79031.1"
                     /translation="MTFNQLQRIDLAVRRRLTLADFAARLADLWGDRVLVDEFGGERL
                     TFLEAARCVERWSNGIAASIAPGDRVVLATPNSYEQFLLCLAV"
     gene            complement(7599..9839)
                     /gene="pcrA"
                     /locus_tag="PKY13_03550"
     CDS             complement(7599..9839)
                     /gene="pcrA"
                     /locus_tag="PKY13_03550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HOT79032.1"
                     /translation="MAAGELLEGLNPSQYEAVTHVGGPLLVVAGAGSGKTRVLTHRIA
                     HLIDAHGVSPFEVLAITFTNKAADEMKHRVGSLIGSVAQKMWVSTFHSACVRILRRDG
                     YHLGYPQQFTIYDQSDAVRLVTYVLRDLQIDPKKLPPRSVHAAISTAKNRGLGVEEYA
                     EAAQVIIERRIADVYREYQARLLRAGAMDFDDLLGQAVEVLRRRPDVLASYQQRFRHI
                     LVDEYQDTNPVQNELIMLLGHEHRNVCVVGDGDQSIYAFRGADLTNILEFERAFPDTT
                     VVVLAQNYRSTQTILDAANAVISHNESRKPKELWSEGATGDKLVRFNAQDESEESQWV
                     AREITRMHDAAEYRWGDVAIFYRTNAQSRVLEEQFMRSGIPYKVIGGTRFYDRREVKD
                     AIAYLRAVVNPIDEVSIKRVLNVPKRGVGDSSIAKLDAYARRESISFLDALRGHVAAG
                     VSGRAAKGVDAFCRLLDGLAPLAEQGPGELLEQALERSGYLEELQNENTIESEGRLEN
                     LSELVGVARDFESIDEFLEQVSLVADTDQIDDDESTVMLMTLHAAKGLEYPVVFMLGM
                     EDGIFPSQRTMSEPDQMEEERRLAYVGITRAREKLFLSAAQSRMLHGTTQYNPPSRFL
                     QEIPEILVDEVGTRRRTVRRSFSSGDDWGSRDSWSHDDTGGRVFGGRASSSSVGEPKP
                     KPATPTGANEIDFRVGDSLRHTQWGEGMILDLRGQGPDTQATVRFADGGAEKVLLLKW
                     APVEKI"
     gene            complement(9886..11085)
                     /locus_tag="PKY13_03555"
     CDS             complement(9886..11085)
                     /locus_tag="PKY13_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOT79033.1"
                     /translation="MSSFSPQKALSVRRPAGLAVRGAAITLFVTLLPGVAGAQPVDRQ
                     QSIGTLQEQARKLAAELDQLDQKTNDLDEQYNDARLQLDGLRQKLADNQAQVDAARAQ
                     LDTNRADAQRYAIEAFVGNSDDSGLVGIADDAIEESQRRTYLASRYGDSEQVVEGLVA
                     AQKDLADQEADLSAANDKVEAKQAEIENAKTQLEATIKNRRDLAAGVQGQLEEALKAE
                     QARLAAEAEAKAKAEAEAAAKRAAAARRVPAVAVQAAAPGSSGSAARAMTVVASSPPA
                     AASTTPVPAGAPTAVEVALAQQGDPYRWAASGPDSFDCSGLIMFAYKAAGVSLPHSSR
                     ALRGMTERISVDELQPGDLVFGGSPVHHVGMYIGNGQMVHAPHSGDVVRVASVYSTSK
                     PVSFGRL"
     gene            complement(11187..12761)
                     /gene="guaA"
                     /locus_tag="PKY13_03560"
     CDS             complement(11187..12761)
                     /gene="guaA"
                     /locus_tag="PKY13_03560"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT79034.1"
                     /translation="MSDAPATDPVLPEGLHPETVLVVDFGAQYAQLIARRVREARVYS
                     EIVPHDVTAAEIARRAPSALIFSGGPKSVNVEGAPGIDPGVYDLGIPILGICYGAQLI
                     ARDLGGTVNNSGRGEYGRCDLTIVGREVLFDPEEAHQSVWMSHFDAITEAPEGFVVTA
                     SSADAPVAALENEARRIYGVQYHPEVAHTPRGQALLERFLYVAAGCSPTWTMDNFIDV
                     AVQQIRDQVGDGRTICALSGGVDSAVAAALVHKAIGDQLTCVFVDTGLMRKNEGDQVV
                     ETFERHQGIELIHVKAADRFFDGLAGVTEPEAKRKVIGELFIRIFEDAKAQVDDAKFL
                     VQGTLYPDVIESGTKHAAKIKSHHNVGGLPEDVDFDLVEPLRALFKDEVRQVGTQLGL
                     PDEIVQRQPFPGPGLGVRIIGEVTPDKVAILQEADAIVREELHAAGLERSIWQSFAVL
                     PDIRSVGVMGDERTYGYPAIIRAVTSDDAMTADWARIPYEVLESMASRIINEVPGINR
                     VAYDITSKPPGTIEWE"
     gene            complement(12758..13606)
                     /locus_tag="PKY13_03565"
     CDS             complement(12758..13606)
                     /locus_tag="PKY13_03565"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT79035.1"
                     /translation="MAQIEIGRGKAARRGYGLDEVSIVPSRRTRDADDVDTAWRLDAF
                     EFDVPLVTIGTTPIDGGLEVIDAEAHYADGAFDAAALTAAVTARNDSGEVSAVAVRPQ
                     RAEELLAALGRTEPDLLVIRGRVVSAEHVSRTREPLNLKRTVRHLEVPVVVGACSSFQ
                     AALHLMRTGAAGVIVASDPHSEGVGVPLATAIADARAARMQHLDETGVYVQLIASVPV
                     HTGGDVAKAIVCGADAVLVEAPDASGVPGDGLRKTVIGDLRRTMATCGFESVKELQSA
                     ELVVIS"
     gene            complement(13734..14126)
                     /locus_tag="PKY13_03570"
     CDS             complement(13734..14126)
                     /locus_tag="PKY13_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79036.1"
                     /translation="MPTLSFEGETHAEIVIKVRRWLASLDGEDEGNISAVEAIEQGAE
                     LTKDALRIIASAAPAPIAQSDLLKGLTNMGYKATDATKEALVGGLDSLEEATGGSVVR
                     QVQKAGRRAAYEMNATIARQLLKSLRGG"
     gene            complement(14193..14942)
                     /locus_tag="PKY13_03575"
     CDS             complement(14193..14942)
                     /locus_tag="PKY13_03575"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOT79037.1"
                     /translation="MAAEGGRGGPQQIPRPTTVRAGGPPPWSDWIDTDPALGLDDVRR
                     RLSAVTVEGAMATVAPDLRRSAVAVLISSLTRAGGVAAGGGSGVEVLLTRRAWTMRTH
                     RGEVSFAGGAEEDGDDFPVGTALREASEEIALDLGLVEVVGALRPLTTFTSDRVVLPV
                     VAVTPERPVVVAEPAEVDAILHVPLAELLHPECYHEERWTWGDEMRGAGISAVTDHPM
                     HFFELVGDTIWGATAAMLHQLLTLLLPAAEA"
     gene            15040..>15509
                     /locus_tag="PKY13_03580"
     CDS             15040..>15509
                     /locus_tag="PKY13_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOT79038.1"
                     /translation="MPATPTTLAPAAALRELKVDVLGIGITPATIALAADEIERWIAE
                     GTPDYVCVTGMHGVVEAQSDPALRDVHNAAGMVVPDGMPMVWASHRVGIPWAERCYGP
                     DLMLEMCRRAAATGHRFYFYGGAEGTPERLRDRLVEQFPGLQVVGLHSPPFRPLT"
BASE COUNT         2417 a         5411 c         5190 g         2391 t
ORIGIN      
        1 gcctcgtcct gcgccggcac accctcggtg atgcagacga tgaactcgat gccaccctcg
       61 gcggcctcca tgacggcggc gcgcacgccg ggcgccggga tgaagatgca gctggcgttg
      121 gcgccggtct cggcgaccgc gtccttgatc gtggcgaaga cggggatgcc gtcgacgtcg
      181 gtaccggcct tcttggggct ggtgccagcc acgacctgcg tgccgtagtc acggttgagc
      241 aggccgtagt agcggccctg gctgccggtc aggccctggt agacgacctt ggtgttctca
      301 tcgacgaaaa tcgacatcgt tctgactcct cgagatcttg tggtcgttgc tcagcgagcg
      361 gcggcgagtt cgacggcctt gcgagccgct tccaacatgg tgggcgccat ctgcaactga
      421 tcgttcaggt gcggctggag gatcgaacga ccctcctcgg cgttggtgcc gtcgaggcgg
      481 atcacgatcg gagcgtcgat ggtgacgcga ccgagggcgg tgatgatgcc gttggcaacc
      541 tcctcgccgc gggtgatgcc accgaagatg ttgatgaaga tcgacttcac acccgggtcg
      601 ttgttgatga cctcgagcgc accggccatg acgtcggcgt tggcgccgcc gccgatgtcc
      661 aagaagttgg cggccgcgcc gccggcctgg ctgaccacgt cgagggtcga catcgcgagc
      721 ccggcgccgt tggcgatgac gccgaccgac ccgtcgaggc cgacgtactg caggcccttc
      781 tcgtgggcgg cgtgctcgcg ttcgtcgcgt acctgcgtgg cgtcgtaggc ctcgtactcg
      841 gggtgacgga acgtgctgtt ggcgtcgagc gtgaccttgg cgtcgagcgc gtgcacctcg
      901 ccggtcggct tgaggatgag cgggttgatc tcggccaggt cggcgtcgcc ctcggtgtag
      961 gccgtgtaca gcttcaggag gatgtcgact gcaccctcgg tggcggcggg gttgagcttg
     1021 gcggcggcga cccactcgcg ggccacggcc tcggtcagcc cgtcgacggg atcgatccag
     1081 atcttcgcaa tggcgtcggg gttgtcgacg gccacctgct cgatctcgac accaccctcg
     1141 gcggacagca tgccgaggtg cttcttcttc gcccggtcga gcgtgaagct cgcgtagtac
     1201 tcctcggcga tgtcggaggc gacctcgatc cagatgatct tgacgatgtg gcccttgatg
     1261 tcgaggccga ggatgttggt cgcgtgctca cgagcctcgt cggcattcgc cgcgagcttc
     1321 acaccgccgg ccttgccgcg cccaccgacc tgcacctgcg ccttgatgac gaccgggtag
     1381 ccgatgcggt cggcgatggc gacggcctca tcgacgttgt cggtcgctgc gccgtcggac
     1441 accgggatgc cgtactgcgc aaagaactgc ttgccctgat actcgaacag gtccacgcct
     1501 gatcctccac gtcatcggtg ggccgccgat cgacggcccg gcatacgggg ggcgatctta
     1561 cgggcgcatg aggtcgagcg cccaacccga cctgacgggg cgtcagattg atgcggtccg
     1621 tgagtgtgcg gcctacgaga gaaaatcgag ggtgcgttcg atggcggggc cgatctcgtc
     1681 gggtgtgaag gccggcggcg ggaacaccat gcgcgacacc cccagctcga cgagcgcctg
     1741 gcgcttctcg ggcgacatcg cggcaccggt ggtgatctcg atcgcatcgg gatcacggcc
     1801 cgccgactcg gccgtggtgc gcatcgtctc gatgagctcg ccgaggatct cctggcggcc
     1861 ggggaagaac ccgtcgccca gtcgaccggc ccgccgcgcc gcggcatggg agtgaccacc
     1921 aaccacgatg ggcggcgaac cgtcatgggg cttggggaac gacatgaccc gctcccagtt
     1981 cacgaactcg ccggcgaacg acgtctcctc ggtcgtccac atctcccgca acgctgcgat
     2041 gtactcgtcg gtccgttgcc cacgacgatc gaacgggacc cccagcgcat cgaactcctc
     2101 ctccagccac cccaccccga taccgagctc gactcggccc ttcgacagcg cgtcgagcgt
     2161 cgcgacctcc ttggccagca ccgccgggtt gcgttgcgga aggatcaaca cccccgtgcc
     2221 gagcctcacc ttcgacgagt tcgccgccac ccacgtcagc cagatgagcg ggtcgggaat
     2281 gggcgagtcc tccgagcccg gcatcttgcc ggtcgagctg taggggtact ccgactcgta
     2341 gcccccaggg accaacacgt gctcgaccgt ccacaccgac tcgacgccgt gggcatcaca
     2401 cgcctcggcc aacgcgaccg cgccctccgc agttccgagg ggtccggtat tggcgaagac
     2461 gatcccgtag cgcatcccgc gaccctactt cggcagcctc ggccacctcc ggggaagcgg
     2521 cgaccgcctg gctcgaactc cgcacggacc gcgagactgt acgagccatg ccgatcgaac
     2581 gccgcagctc cagcgcccag atccgatcga tcctgatcgc gatcgtcacc gtcgtgttcg
     2641 tcgccacgct cggctacgcc gtgttccgcg ccgcgaccgg ccagaagacc tcggtcacca
     2701 agggctccga cggcggacgg tggaacaccg ggacggcaca gaccaaggcg gacgagatcg
     2761 ccgagcgcgg gccgatcctc attcccgatc cggccggtgc gcagcgcctc cccatctaca
     2821 tcagccacat cggcactgac cccgacaccg gctggtacgt cttcgaggcg cgaccgcccg
     2881 acgcgcccga cgactgcttc atctcatggg accgtgacgc agaggagttc acggcaccgt
     2941 gcgtcgacga cacctatcct gccgacggcg acggcctccg caacttcgag gcaacggtga
     3001 cgaaggacgg cgacctcatc atcgacttca cccccgggcc gacaacgacc accacgagca
     3061 gccccgaata atcgctggac tggtcgcgcc caccgagggg tagggtcgtc ctcgtgaaga
     3121 tctgagcgag cgtcccatcc cgcgtctgac cgacccgacc ctgctgtcca tgcagcggca
     3181 tcggaatccg gtccgcggat gccgcccgcc gtccacgttc ctcgggaggt cccatgacct
     3241 tgcacgctca ccccttctcc cccaccgacc cggtcgtcgt cgcgaccaac gacccagcga
     3301 ccgaacccgc catcgcgtcg gtcgagatcc ggggcgccgg tctgtccaag gccttcggcc
     3361 ccgtccaggt cttcgacgac gtctccctcg tggccgcgcc cggccaccgg ctcggcatcg
     3421 tcggtgagaa cggtgccggc aagtcgaccc tgctcgcgat cctgtgcggc acgctcgagg
     3481 cggacctcgg cgacgtccac cggccggccg gcgtcgggta cctgcatcag gagttcccgt
     3541 tcccgccgga caccgacgtg gccaccgtga tcgaggcggc cctcgccccg gcgcgcgcca
     3601 tcgaagcgga gctgagtgca gcggcatccg cgctcggcga cgtcgacgac gccggagcga
     3661 gccgacggta cgagcttgcg ctcgcggcgg cagagcgggc cgacgtgtgg aatgccgatc
     3721 accgcgccga cgaggtggtc acggggctcg gcctgcaacg ggtcgacccg ggacggacca
     3781 tcggctccct gtcgggcgga gaacgcaccc ggctcgagct cgcgacgctg ctcatcgccc
     3841 acccaccggc gatggtgctc gacgaaccga ccaaccacct cgacgacgag gccatcgact
     3901 tcctgacccg gttcctcgtg gcgttccccg ggccggtggt gaccgcgagc cacgaccgct
     3961 tgttcctcga acaggtgtgc accggcatcg tcgacctcga cggcggacgc gacggcgccg
     4021 cccgcaccta cggcggctcc ttcgagcggt acctcgacct caaggccaag gaacgagccc
     4081 gatgggccca gcagtacgcc gacgaacagg accagctcga ccgactccgc cacgacatca
     4141 cccacgtcgc gcccaacgtc gcccacaacg cccccaggac cgacaacgac aagttcatcc
     4201 accacttcaa gggcggacgg gtcaacaacc agatctcgcg tcggttgcgg aacgcccggg
     4261 agcgactcga cgaactcgag gagcgacagg tccgcaggcc gccggtcgcc ctgcacttcg
     4321 aggcgccgac gacaaccggc acccagtcgt tcgaaccgct gatcggcgcc gtcggtgtgt
     4381 cggtcgagca ccggctcgcc cgggtcgacg tggcggtcga cgcgcagacc cgcctgctcg
     4441 tcaccggagc gaacggggtc gggaagtcga cactgctcgc agcactcgct cggcgcacac
     4501 cgacgacgac cggcaccgtc acctggcgca agggactccg catcgggtac ctcccccaag
     4561 acgtcgtgtt cgacgacccc gacctcacgg cggaacgcgc cttcgacgca gtggcccgcg
     4621 gcgtgaccct caccgacctc ggactgctct cggggagcga cctctcgcga ccgctgggtc
     4681 agctcagcgt cggtcagcgt cgacgggtgg ccctcggatg catcgtggcc aatcgccccc
     4741 acgtcctcgt gatggacgag cccaccaacc acctctcgct gaccctcgcc gaggagttgg
     4801 aggaagcgct gatgaccacg ctcggcgccg tcatcgtcgc cagccacgac cgctggctcc
     4861 ggcgccgctg ggacggggac gagctccgcc tcgagcgagc gggcgccggt gacgagaact
     4921 gaacgcgggc ggagccggag ctggatcagg gcgcgagctg tgccgcgatg tcggcggcga
     4981 tggcgggccg aaggtggcga tcggcgagct tgccgaccac ctcgaacacg cggttgatcg
     5041 ccagttgccg gtagccggca gaccggtcca tcacgaggtc gactccctcg acgtcggcgt
     5101 cgcgttcggc ggaacgaccc tgggccgccc agatcgcatc ggcccggtga taggtgaaca
     5161 tgcggcccgg gttcgtagcc tcgacgagac gccgcagctc ctcgctcccg gtgccctcga
     5221 tggcaatctc ggtgagccga acccgcgact cggccatgtt cgctgctcgg gtcgagtggc
     5281 cgacgcggta gtagtagttg gcctgcgcga cacgaacggg tggcggcgaa ccgggcgcga
     5341 acgcgacctg gaggtagaag gcgaggtcct cccccgccga caggccctcg gggaaccgga
     5401 tcgcacgacg ttcgagcagg tcgcggcgaa tgaacgcccg catgtggcac tcgcggtcgc
     5461 ggaaccaggg ccccaatcgc aacgggtggg tgcctcccat ccaccagctg acccgactca
     5521 cgtatcgctg gccgagcgac accgagccgt ccggctgtgg ggtgaactcg atgaggtcgt
     5581 cggtgaacgc gtcgaccgcc gggtagcggc gtgaggctgc caccagctct cgcagccgac
     5641 ctcggaacca caggtcgtcg gcgtccatcg gacacaccca cgttccgcgg gcgacgtcga
     5701 ggccggcatt gcgcgccgcc gaccgaccga cgttggcgcc gaggagcagc ggccggaccc
     5761 gctcgtcgcg ttcggcgaac tcgagcagga cgtcctgggt ggcatcggtg gagccgttct
     5821 ccgcaacgat gacctcgaag tcgccgaacg tctggtcgag gatcgagcgg agcgtcgtcg
     5881 caaggtaggg ctccgcgttg tacgccggga tgacgacgga gacgaagggg tcaggtgaag
     5941 agctcaacga gccgttcctt ctgcactttc tcggtgccgg tgcgcgggag ctcgtcgacc
     6001 acgatcaccc gtcgcggcgc cttgtacgcc gcgaggcgct ccgcagccca cgccgcgatc
     6061 tcgtcgccgt cggccttggc ccggcgtcgc acgcgcaccg ccgctgcggg gacctcaccg
     6121 agcttcgcgt cgggaatgcc caccaccgaa gcctccacga cgtcgggatg ctcctcgagc
     6181 agtgtctcga cctcgagcgg atacaccgaa tagccgcccg acttcaccgt gtgcttgcgc
     6241 cgaccgcaga acatcacggt gccgagcgga ccgacgcgaa ccaggtcgcc ggtgcggagc
     6301 caaccgtcct cggtgacggc tgcctccgtc gcggccgggg cgttccagta gccggtgagg
     6361 acgccgggac cacgcaccca caactcgccc agaccgccca tcgagacctc ggcgtcggac
     6421 tcgtcgacca cccggaactt gtagccgggc agccggaacc cgaccgtttc gccgacgccg
     6481 agcggcaggt acggcggcga caccttcgcc gcgacaccgc ctcccacctc gaccatcccg
     6541 tagccctcca cgaacagcgc ctcccccagg tcgccgacga ggggaacgtg gagcgacgct
     6601 ccgaacttct tgaacgaacg tgcgagctcc gacggcatga cgtcggcgcc cgacatccac
     6661 acccgcacgc tcttcagatc gtggcggtcg gcaccggcct cgagcatcat ccggtacatc
     6721 gccgggaccc cgacgaaccc gctgcaacgg cgggattcga tcgcgttgag cacgtcgttg
     6781 gggcggaacc gtcgcatgaa gtacacggga agggcggcgg tggcgagacc caccagcgtg
     6841 gcaaacccca tgatgtgggc gaccggcaac gccacgacga cctcgtcgtg acgcagctgg
     6901 accggccaga gcgccgctgc cgccgactga cccacgagtg cccggtgggt gagcgcgacc
     6961 cccttgggct tgccggtcgt gccggacgtg tagaacagcg ccgcgacgtc ggacgtggcc
     7021 ggtgttgccg gatcgcacgc cggggccccg ggatcgagat cgagatcgcc ggcgtccttc
     7081 acgacgaacg aggcgccgct gtcggcgatc acgtggtcga tctcggccgg tcgcatctgc
     7141 gggttgaccg gcaccggcag cccgccggcg cgcgagaccg ccaggcagag gaggaactgc
     7201 tcgtaggagt tcggggtggc caacaccacc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn naccgccagg cagaggagga actgctcgta ggagttcggg gtggccaaca
     7381 ccacccggtc gcccggcgcg atcgaggccg cgatgccgtt ggaccagcgt tcgacgcacc
     7441 gagccgcctc caggaaggtc agtcgctcac cgccgaactc gtcgacgagc acccgatcac
     7501 cccacaggtc ggcgagccgc gccgcgaagt cggcgagggt cagtcggcgg cgcaccgcca
     7561 gatcgatgcg ttggagttgg ttgaacgtca tggcggtgtc agatcttctc gaccggtgcc
     7621 cacttgagca gcaacacctt ttcggcgccc ccgtcggcga acctgaccgt tgcctgggtg
     7681 tcgggacctt ggccgcgcag atcgaggatc atgccctcac cccactgcgt gtggcgaagg
     7741 ctgtcgccga cccggaagtc gatctcgttg gctcccgtcg gtgtcgcggg cttcggtttc
     7801 ggttcgccga ccgatgacga cgacgcacgc ccgccgaaca cccgaccccc ggtgtcgtca
     7861 tgcgaccagg agtcgcgcga cccccagtcg tcgcccgacg agaacgaccg gcgaaccgtg
     7921 cgtcgccggg tgccgacctc gtcgacgagg atctcgggga tttcctggag gaaccgcgac
     7981 ggcgggttgt actgcgtcgt gccgtgcaac atgcggctct gggccgctga caggaacagc
     8041 ttctcgcgcg cccgcgtgat gcccacgtag gcgagacggc gctcttcttc catctggtcg
     8101 ggctccgaca tggtgcgctg actcgggaag atgccgtctt ccatgccgag catgaacacg
     8161 acggggtact cgagcccctt cgccgcgtgc aacgtcatca gcatgaccgt gctctcgtcg
     8221 tcatcgatct ggtcggtatc ggccacgagg ctcacctgct cgaggaactc gtcgatcgac
     8281 tcgaagtcgc gcgccacccc gaccagctcc gacaggttct cgagccgacc ctcggactcg
     8341 atcgtgttct cgttctgcag ctcctcgagg tagccgctgc gttcgagcgc ctgctcgagg
     8401 agctcgccgg gaccctgctc ggcgagcggc gcgaggccgt cgaggaggcg gcagaacgcg
     8461 tcgacaccct tggctgcgcg gcccgacacg cccgcggcga cgtggccgcg gagcgcgtcg
     8521 aggaagctga tcgactcgcg ccgggcgtag gcgtcgagct tcgcgatgct cgagtcgccc
     8581 acaccgcgct tggggacgtt gagcacgcgc ttgatgctca cctcgtcgat cgggttgacc
     8641 acggcgcgga gataggcgat cgcgtccttg acctcgcgac ggtcgtagaa ccgggtgccc
     8701 ccgatgacct tgtaggggat gccggagcgc atgaactgct cttcgagcac acgcgactgc
     8761 gcgttggtgc ggtagaagat cgcgacgtcg ccccaccggt actcggcggc gtcgtgcatg
     8821 cgggtgatct cacgggcgac ccactgggac tcctcgctct cgtcctgggc gttgaagcgg
     8881 acgagcttgt cgccggtcgc gccctcggac cacagttcct tcggcttgcg cgactcgttg
     8941 tgcgagatga cggcgttcgc agcatcgagg atggtctggg tggaccggta gttctgggcg
     9001 aggaccacga cggtcgtgtc ggggaacgcc cgctcgaact ccaagatgtt ggtgaggtcg
     9061 gcgccgcgga acgcgtagat cgactgatcg ccgtcgccga cgacgcacac gttgcggtgc
     9121 tcgtggccca ggagcatgat cagctcgttc tgcaccgggt tcgtgtcctg gtactcatcg
     9181 accaggatgt gccggaaccg ctgctggtac gacgccagca cgtcagggcg acggcgcagc
     9241 acctcgacgg cctgcccgag gaggtcgtcg aagtccatcg caccggcccg caacagccgg
     9301 gcctggtact cgcggtacac gtcggcgatg cgccgctcga tgatcacctg cgcggcctcg
     9361 gcgtactcct cgacgccgag cccccggttc ttcgccgtcg agatcgccgc atggaccgaa
     9421 cggggcggga gcttcttggg atcgatctgg aggtcgcgca gcacataggt gacgaggcgg
     9481 accgcgtcgg actggtcgta gatcgtgaac tgttgcgggt agccgaggtg gtacccgtcc
     9541 cggcggagga tccgcacgca tgccgagtgg aacgtcgaca cccacatctt ctgggcgacc
     9601 gaaccgatga gcgatccgac gcggtgcttc atctcgtcgg cggccttgtt ggtgaacgtg
     9661 atcgccagga cctcgaacgg cgagacgccg tgcgcatcga tcaggtgggc gatgcggtgc
     9721 gtgagcaccc gcgtcttgcc cgagccggca ccggcgacga ccaagagcgg tccgccgacg
     9781 tgcgtcaccg cctcgtactg agacgggttc aatccctcga gcaactcccc ggcggccacg
     9841 acgcaacgct agcgggtgcc cctgacggcc tcggccgccc gacggctaca gacggccgaa
     9901 cgagacgggc ttggaggtgg agtagacgct ggcgacccgc acgacgtcgc ccgagtgcgg
     9961 cgcgtgcacc atctggccgt taccgatgta catgccgacg tggtgcaccg gcgacccacc
    10021 gaacacgagg tcaccgggct ggagctcgtc gacggagatc cgttcggtca tgccccggag
    10081 cgcccgcgag gaatgcggaa ggctcacgcc ggcggccttg taggcgaaca tgatcagacc
    10141 ggagcagtcg aacgagtccg gccccgacgc ggcccagcgg taaggatcac cctgctgcgc
    10201 cagcgcaacc tcgacggccg tgggggcgcc ggccggaacg ggagtcgtcg atgcagcagc
    10261 gggcggcgac gacgcaacga ccgtcatcgc tcgagcggcc gagccgctgg agcccggcgc
    10321 agcagcctgc acagcgacgg ccggaacacg acgagcggcg gcagcacgct tggcagcggc
    10381 ttccgcctcg gccttcgcct tcgcctcggc ctcggcagcc aggcgggcct gctcggcctt
    10441 cagggcctcc tcgagctgcc cttgcacacc ggcagcgaga tcccgacggt tcttgatcgt
    10501 cgcttcgagc tgcgtcttcg cgttctcgat ctcggcctgc ttggcctcga ccttgtcgtt
    10561 cgctgcggag aggtcggcct cctggtcggc caggtccttc tgcgccgcta cgagaccctc
    10621 gacgacctgt tcgctgtcgc catagcgcga ggcgaggtag gtgcgacgct gcgactcctc
    10681 gatggcgtcg tccgcgatgc cgaccaggcc agagtcgtca ctgttgccga cgaatgcttc
    10741 gatcgcgtac cgctgggcat cggcgcggtt ggtgtcgagc tgcgcccgag ccgcatcgac
    10801 ctgtgcctgg ttgtcggcga gcttctgcct caggccgtcg agctggagcc gcgcatcgtt
    10861 gtactgctcg tcgaggtcgt tggtcttctg gtcgagctgg tcgagctcgg cggcgagctt
    10921 gcgggcctgt tcctgcagcg tgccgatgga ctgctgacga tcgacgggct gggcgccggc
    10981 gacgccgggg agcagggtca cgaagagggt gatggcggcg ccacggacgg cgagaccggc
    11041 tggtcgtcga accgaaaggg ccttctgggg ggagaacgag gacatgaggg gatcaatggt
    11101 gccaggagcg ggcaattatg acaactcggt gacgaaattg tgacgttccg ttacacggat
    11161 gtcatttacg cgctcaatca tcgccgtcac tcccactcga tggtgcccgg cggcttggag
    11221 gtgatgtcgt aggcgacgcg gttgatgccc ggcacctcgt tgatgatgcg cgacgccatc
    11281 gactccagca cctcgtacgg gatgcgcgcc cagtcggcgg tcatggcgtc gtcgctcgtg
    11341 acggctcgga tgatggccgg gtacccgtag gtgcgctcat ctcccatcac cccgaccgat
    11401 cggatgtcgg gcagcacagc gaacgactgc cagatcgagc gttcgaggcc ggcggcgtgg
    11461 agttcctcgc gcacgatggc gtcggcctcc tggagtatcg ccaccttgtc gggggtgacc
    11521 tcgccgatga tgcggacgcc gagaccgggg cccgggaacg gctggcgttg cacgatctcg
    11581 tcgggcagac cgagctgggt gcccacctgc cgcacctcgt ccttgaacag cgctcgcaac
    11641 ggctcgacga ggtcgaagtc gacgtcctcg ggaaggccgc cgacgttgtg gtggctcttg
    11701 atcttcgcgg cgtgcttggt gcccgactcg atcacatcgg ggtacagcgt gccctgcacg
    11761 aggaacttgg cgtcgtcgac ctgggccttc gcgtcctcga agatccgaat gaacagctcg
    11821 ccgatcacct tgcgcttggc ctcgggctcc gtgacgccgg ccagcccgtc gaagaaccgg
    11881 tcggccgcct tcacgtggat cagctcgatc ccctggtggc gctcgaatgt ctcgaccacc
    11941 tggtcgcctt cgttcttgcg catcaggccg gtgtcgacga agacacacgt gagctggtcg
    12001 ccgatcgcct tgtgcaccaa ggcggcggcg accgcggagt cgacgccacc ggacagggcg
    12061 cagatcgtgc ggccgtcacc gacctgatcg cggatctgtt gcaccgcgac gtcgatgaag
    12121 ttgtccatcg tccacgtcgg actgcagccg gcggcgacgt agaggaaccg ctcgaggagc
    12181 gcttggccgc gtggcgtgtg cgccacctcg gggtggtact gcacgccgta gatgcgacgc
    12241 gcctcgttct cgagcgccgc cacgggagca tcggcggacg acgccgtcac gacgaacccc
    12301 tccggtgcct cggtgatggc gtcgaagtgg ctcatccaca ccgactggtg ggcttcctcg
    12361 gggtcgaaca gcacctcacg gccgacgatc gtcaggtcgc accggccgta ctcgccacgg
    12421 ccgctgttgt tgacggtgcc cccgaggtcg cgggcgatca gctgggcgcc gtaacagatg
    12481 ccgaggatcg ggatgccgag gtcgtacaca ccggggtcga tgccgggcgc cccctcgacg
    12541 ttgaccgact tcgggccacc ggagaagatc agcgccgacg gggcgcgacg agcgatctcg
    12601 gccgcagtca cgtcgtgggg cacgatctcg gagtacaccc gcgcctcgcg gacgcgtcga
    12661 gcgatgagct gggcgtactg ggcgccgaag tcgacgacca gcacggtctc gggatgcagg
    12721 ccctcgggaa ggacggggtc ggtggccgga gcgtcgctca cgagatcacc accagctctg
    12781 ccgactgcag ttccttgacg gactcgaagc cacaggtggc catcgtccgc ctcaggtcgc
    12841 cgatcaccgt cttccggagg ccgtcgccgg ggacaccgct cgcgtcggga gcttccacca
    12901 gcaccgcgtc ggcgccacag acgatcgcct tcgccacgtc gccgccggtg tgcaccggga
    12961 ccgacgcgat gagctgcacg tacacaccgg tctcgtcgag gtgctgcatc cttgcggcac
    13021 gggcgtcggc gatcgcggtc gccaatggca cgccgacacc ctcgctgtgc gggtcgctgg
    13081 ccacgatgac gcctgccgca cccgtacgca tgaggtgcaa cgccgcctgg aaactggagc
    13141 aggctccgac gaccaccggt acctcgaggt gtcggacggt gcgcttcagg ttgaggggct
    13201 cgcgggtccg cgacacgtgt tccgccgaca cgacccggcc acggatgacc agcaggtcgg
    13261 gctcggtccg gcccagcgca gcaagtagct cctcggcgcg ctgggggcga acggcgaccg
    13321 cggacacctc gccggaatcg ttgcgggccg tgaccgcggc ggtgagggcg gcggcatcga
    13381 acgctccgtc ggcgtagtgg gcttcggcat cgatgacctc gagaccacca tcgatcgggg
    13441 tggtaccgat ggtgaccagt ggaacgtcga actcgaaggc atcgagccgc caggccgtgt
    13501 cgacgtcgtc ggcgtcgcgg gtgcgacgac tcggcacgat cgacacctcg tccaggccat
    13561 agccccgtcg ggcggctttg ccccgtccga tctcgatctg tgccatcgca ccgttcctct
    13621 cactccgagc gggccaccgg gggctccgtc agaaagaggc ggatcttagc cgctggaaag
    13681 aagggctctt ccttcggccc gacgtcggag cggcgcgccg ctctcgtcgt ggctcagccg
    13741 ccccggagcg acttcagcag ctgccgggcg atcgttgcgt tcatctcgta ggcggcacgc
    13801 cggccggcct tctgcacctg acgcacgacc gacccaccgg tcgcctcctc gagcgagtcg
    13861 aggccaccca cgagtgcctc cttggtggca tcggtcgcct tgtatcccat gttggtgaga
    13921 cccttcagca ggtcgctctg ggcgatgggc gccggagcag cggacgcgat gatgcgcaac
    13981 gcgtccttcg tcagctctgc gccctgctcg atcgcctcga ccgcgctgat gttgccctcg
    14041 tcctcaccat cgagggacgc caaccatcgt cggaccttga tgacgatctc tgcgtgggtc
    14101 tcgccttcga acgacagggt gggcatcgca ggctcctcgt acggcgggct gctccgaggt
    14161 tagcggccct cggccccgat tccccgggac ggtcaggcct cagcggccgg caggaggagc
    14221 gtcaacagct ggtgcagcat cgccgccgtg gcaccccaga tcgtgtcacc gaccaactcg
    14281 aagaagtgca tcggatggtc ggtgacggcg ctgatgccgg cgccgcgcat ctcgtcgccc
    14341 cacgtccacc gttcctcgtg gtagcactcg gggtgcagca gctctgccag cggaacatgc
    14401 aggatcgcgt cgacctccgc cggctccgcc acgacgaccg gtcgctcggg cgtgaccgcc
    14461 acgaccggga gcaccacccg atcgctcgtg aaggtcgtga ggggtcgaag cgctccgacg
    14521 acctcgacga gcccgaggtc gagggcgatc tcctcgctcg cctcacggag ggccgtgccg
    14581 acagggaagt cgtcgccgtc ctcctcggcc ccaccggcga acgacacctc gccgcgatgg
    14641 gtccgcatcg tccatgcccg acgggtgagc agcacctcca cacccgaccc tcctccggcg
    14701 gcaacgccac cggcccgagt gagcgacgag atgagcacag cgaccgcgct ccggcgaagg
    14761 tcgggtgcca cggtcgccat cgcgccctcg accgtcacgg cggacagacg tcgccgaacg
    14821 tcgtcgaggc cgagcgccgg gtcggtgtcg atccagtcgc tccacggtgg cgggccaccg
    14881 gcacggaccg tcgtcggtct cgggatctgt tgtggcccgc cgcgaccgcc ctccgctgcc
    14941 atcggcgcac ggtaccaggc acggcgaacc gtcgagcgcg gccgccgagc gtcggatccg
    15001 tcgtgccaga tttctcagga tcgccggtag aattccctgg tgcctgccac ccccaccacc
    15061 cttgctcccg cagccgcgct ccgcgagctc aaggtcgatg tgctcggcat cgggatcacg
    15121 ccggccacca tcgccctcgc tgcggacgag atcgaacgct ggatcgccga aggcaccccc
    15181 gactacgtgt gtgtcacggg tatgcacggc gtcgtcgagg cccagtccga tccggcgctg
    15241 cgcgacgtgc acaacgccgc cggcatggtc gtgcccgacg ggatgccgat ggtctgggcc
    15301 agccaccgcg tcggaattcc ctgggccgag cgctgctacg gccccgacct gatgctcgag
    15361 atgtgtcggc gcgccgccgc cacgggccac cgcttctact tctacggcgg cgccgagggc
    15421 acgcccgaac gcctccgcga ccgcctcgtc gaacagttcc ccggcctcca ggtcgtcggg
    15481 ctccactcgc cgccgttccg ccccctcac
//
LOCUS       DAOSOA010000030        15192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3934_length_15192_cov_5.617956, whole genome shotgun sequence.
ACCESSION   DAOSOA010000030 DAOSOA010000000
VERSION     DAOSOA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15192
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3934_length_15192_cov_5.617956"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKY13_03585"
     CDS             <1..289
                     /locus_tag="PKY13_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79039.1"
                     /translation="NNAFTDICEIARDEGGIVTLAINAGLPLPDLVITLGRDGCVSPP
                     AFEGPTVWPITRHFVTAALRFNAGLDPEPVGLGSQVVGEFSPAPSTYLHRP"
     gene            326..1357
                     /gene="ispH"
                     /locus_tag="PKY13_03590"
     CDS             326..1357
                     /gene="ispH"
                     /locus_tag="PKY13_03590"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOT79040.1"
                     /translation="MAVDRVLLAAPRGFCAGVEMAIKALAWMVRAFEPPVYCYHEIVH
                     NQRVVQRFRDLGVVFVDDIAEVPEGAPIMLSAHGSAPEVVAEAMARGGYVVDSVCPLV
                     TKVHHEVKVRAGKGYQIVYVGHEGHEEAVGTMAVAPESTHRVESIAEVAALPAFETPV
                     ALLAQTTLSHRDWRDVLDATKERFPDMWVPGRSDLCFATTNRQTALMNIAPRCDAMVV
                     VGSANSSNTLALEKLAREAGCPQVFRINDADELPDELSGIVGVTAGASAPEDLVEAVI
                     ARLAPANGVEEVRTTDEDEYFPPPRNLRDLLLAVDSFATFALGGDPVDRPGLNDRKLP
                     ASDVLSSLA"
     gene            complement(1396..2295)
                     /locus_tag="PKY13_03595"
     CDS             complement(1396..2295)
                     /locus_tag="PKY13_03595"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOT79041.1"
                     /translation="MDQLRLMRCATRDHGVLLPDVLDREGVSAKQRRHRVQTGEWRRL
                     PCGMLVIAGVPETFEMLATAALAAVSGSVLGHHSAGRVHGVEVDDRVVHVCVPPGSTG
                     RCRGVAVHRSALDAIDVTRRRGFPVTTLERTLVDLGATLPPRQLSSVVEAALRDRLTT
                     FDRVERVFSRVSRQGRPGTKRMRDVLVRLDGTPPTESELETMFLDLIVGAGLAIPQSQ
                     VTFDWAPGEAGRVDFWYEAARLVVELDGRRFHARLEAFERDRRRDQLALMNDQRPVRF
                     THHQISDSPDEVVEVVRSLLAKP"
     gene            2480..2848
                     /locus_tag="PKY13_03600"
     CDS             2480..2848
                     /locus_tag="PKY13_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79042.1"
                     /translation="MDTFRLFLHVLAASVWVGGQIVLGGLVPTLRKISPEAPKLAAQA
                     FNRIAWPAFGVALVTGIWNMLVVEDLDQALFGIKFLLVIVSGAGAAIHIVGKSKAALA
                     VGGALASVGAIAAMYVGLAL"
     gene            complement(2850..5495)
                     /locus_tag="PKY13_03605"
     CDS             complement(2850..5495)
                     /locus_tag="PKY13_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT79043.1"
                     /translation="MLKPVRAVWVERQRWFGLLAAVVVSVAFVTATLALGGLTRSTLD
                     GALDGRFGGVDVVLRAGGGQEIGGAALRAAVPGDVASAMAASGDLVAVAGVIEGAATV
                     LGRDGRPIDELFGAGTVATNWLVDPELAGVEIVEGRAPVADGEVVLDTGTAAVAGVVP
                     GDEVRVDFPVASRRLTVVGVAAIVRDGEIVPGPRLVWMDEPTTRSALDKSSGFDFVIG
                     RAGGDLGDGALERLRSRIGSELGADSSADSDSSAGVEVVTGSTLVGERQAVVRRIAEF
                     FDRPMMASGFIAVFVGAFVIFNTFSIVMAQRVRELGMLRAIGATRAQVTRTVLSEAVV
                     IGVVGSVVGLAAGWLAAWSTRSVFARALDLPDGPLGVGWGSVIAAFATGLGSTLVAGI
                     VPAVRAGSTSPMSVLRSTQVDDTYRWGRRHWSGLALLVAGVTLISLILTGAAELGLAG
                     IGVGSAAVFIAFAMVGSAVVGPVFGWVGRPLVAWHGLTARLAIRSAARSPGRTTLVIV
                     ALSIGIAMMTVITTFEASVRAATEDRAAGQLAGIDLLVESPVGVPRTVAARLSELDGI
                     DAVTPVRLGVMTVLNSYDAAQARDAAGVDAATASSAVPPIGAAKFLTGVDLDAASRMI
                     EVPGLSPPITHLDDGEVMVLAKTAERAGWRVGDDVEVWFATTGRATLRLAATFTGRFG
                     VDAEYVANLATTSSNAPPEFSGDSVVWAKADDRASVPEVVAAARRELRALAPGASVTS
                     VQEYLGQRMTGINSLINLLVVLVGVSVLVAWVGVANTVALALRERRTETTRLRALGLT
                     RSQFHRTVVIETLLIAVFGSALGVVTGWSMAAGFITSMGEEGLRPVVDPVSIGGIALL
                     GALAAIATAAYQARRVSGPVLPAGR"
     gene            complement(5620..6279)
                     /locus_tag="PKY13_03610"
     CDS             complement(5620..6279)
                     /locus_tag="PKY13_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT79044.1"
                     /translation="MKPSPRGPVALAAYRVLWALARGLTGAYWRLEVIGGERVPRSGA
                     FVVAPVHRSNLDFLLAGLAVKRPVRYMAKSSIFLGGLVDRFLNVMGAFPVVRDTADFS
                     AIRTCEDLLAAGEPVVVFPEGRRKSGDDVADLFDGPAFVAARQRVPIIPIGIGGSDKA
                     MPIGKKWILPHKVVIVIGEPIYPDVDIEGRVPRSAISALTEQLRGEVQGVYDEARRLA
                     G"
     gene            complement(6276..6968)
                     /gene="cmk"
                     /locus_tag="PKY13_03615"
     CDS             complement(6276..6968)
                     /gene="cmk"
                     /locus_tag="PKY13_03615"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOT79045.1"
                     /translation="MNENADIGNVIAIDGPAGSGKSTISKMVAHRVGLPMLDTGAMYR
                     AVTCAVLRAGGDVHDAEGATASAERAEIVVDERVTINGDDVTTEIRGPEVSANVSIVA
                     THPGVRRRLRGLQREWMARRGGGVLEGRDIGTVVLPDAPLKIYLTASPEVRARRRAGD
                     DLGDFDLEAAAANIAERDRIDSTRADSPLRPADDAVIVDTSDLTIEQVVDRIVAEAEA
                     RGLGRVARKEPS"
     gene            complement(6965..8284)
                     /gene="aroA"
                     /locus_tag="PKY13_03620"
     CDS             complement(6965..8284)
                     /gene="aroA"
                     /locus_tag="PKY13_03620"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT79046.1"
                     /translation="MIGIDWNDLASLPDPLPIEPLSGPIPDVTVPIPGSKSITNRALV
                     CAALGNGPSVLTGILDSDDTNAMLEALRTLGIQIEVDGDRASVWGCSGRLPVRSATLD
                     VRMSGTTSRFLLPLAALGKDSITLDGHESMRARPMADGIAAIEALGRSVTSNDGCLPV
                     TVAPDPDVDELAGSVEVRGDVSSQFLSGLLLIAPCLPDGLEITVDGPLQSVPYVDLTV
                     AVMRAFGAKVEVADDRRWFAVEPTGYQQISYDIEPDASAASYFLAAAALCGGSVTIDG
                     LGSDSLQGDVRFAEVLRSCGADVELRPGSVTVTGGALSGGVFDLADISDTAQTLAAIV
                     PFASASVTVEGIGFIRRKETDRIHAVVTELQRLGIGATELDDGFVIEPGPVSPGTVQT
                     YDDHRMAMSFAILGLKVPGIAIADPGCVAKTFPGFWATLELLRGAPR"
     gene            complement(8281..9351)
                     /locus_tag="PKY13_03625"
     CDS             complement(8281..9351)
                     /locus_tag="PKY13_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOT79047.1"
                     /translation="MSEQRRAQVIGTGLIGGSIGMALRARRWHVTGHDQSNFNAQHAR
                     ERGAIDEIGLDDRASICFLAMPVSGISSDGRRVLEETDWVVTDVGSVKGPIVAALDSP
                     RFVGGHPMAGSEQDGIQGSSASMFEGAVWVLTPTATTDPKAQAMVHSVVASFGADVIT
                     LDAAVHDQFVAMVSHVPHLTAATLMGLAGQRSTQEGRGALLRLAAGGFRDMTRIASGS
                     PYIWPDICGDNREAIVDVLDDLIRELTEVRSIVDGNDRNALIDRLESAREGRLNLPTT
                     ATRPDELVELRVGVPDRPGVLAAVTAMATQHEVNIYDIEIAHSAEGPRGVLILLVDSG
                     KVDTFVESLFADGYAVSQRALT"
     gene            complement(9362..9730)
                     /gene="aroH"
                     /locus_tag="PKY13_03630"
     CDS             complement(9362..9730)
                     /gene="aroH"
                     /locus_tag="PKY13_03630"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOT79048.1"
                     /translation="MPTSVRALRGATTVDVDDADHLSDRVVELVTEMLERNGIAHDAL
                     ISIIFTATEDLTSTFPATAARKVGLGDVPLICARELSIDGAMPRCVRVMMHLETELGR
                     DELRHVYLHDARSLRDDLPE"
     gene            complement(9764..10501)
                     /locus_tag="PKY13_03635"
     CDS             complement(9764..10501)
                     /locus_tag="PKY13_03635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT79049.1"
                     /translation="MSGEPGGLPEGERLQKVLAQAGIGSRRVCEIYIDEGRISVNGVI
                     AHLGQRVDPLVDVVEFDGSQIGVKPGLVHYLLNKPAGVVTTAQDTHGRPTVVELVPAE
                     PRVFPVGRLDADTEGLLLLTNDGDLTHRLTHPSFGVDKEYLAEVEGEPSRGALRRLRE
                     GVELEDGSTAPATAALLAPNLIRLVIHEGRNRQVRRMCDAVGHPVVRLVRTRIGPLSD
                     TTLAPGAWRELTQQEVRSLERAAGAAE"
     gene            complement(10498..11289)
                     /gene="scpB"
                     /locus_tag="PKY13_03640"
     CDS             complement(10498..11289)
                     /gene="scpB"
                     /locus_tag="PKY13_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOT79050.1"
                     /translation="MSESEPIEHRSPLAAEAKRALEAIVMVSDEPADPRLLAQLIEIP
                     AARVDELCVELAAEYDAQQRGFALVKVAGGWRFQSRAELSPYVERFVLTGQSARLSAA
                     ALETLSIVAYKQPISRAQVAAIRGVSVDGVMRTLQQRGYIGELARDPGPGQAVLFGTT
                     SLFLERLGLNSLDELPPLGDFVPGPDVVEALEAGLRVNEDSAHDAPAQSAAHEAAAHE
                     AAVHEADEPVIDLRDQTPVTEAPVTEAPVVEAPLSLDALDVLADD"
     gene            complement(11293..12072)
                     /locus_tag="PKY13_03645"
     CDS             complement(11293..12072)
                     /locus_tag="PKY13_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOT79051.1"
                     /translation="MAIDVQTDVFQGPFDLLLHLILRDQVDLYEVSLTKIVDAYLTHL
                     SEMSEMDLDVATEFLLIAATLVELKARRLLPGLDDGDLDDELGIWEERDLLLARLLEC
                     KTFKDAAIVLADLHASAGQCFARTVGPDERFIDLTPDSLVGVTPLQLRSAFMKATAPK
                     PEPRIDLFHVAPIRASVVEAVAELIDELPRIGRISFRDLTAAFVEKLEIVVRFLAVLE
                     LFKQGYIDLDQPTNFGDITIEWTGGELGDGADLALVDMYEG"
     gene            12148..13146
                     /gene="trpS"
                     /locus_tag="PKY13_03650"
     CDS             12148..13146
                     /gene="trpS"
                     /locus_tag="PKY13_03650"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT79052.1"
                     /translation="MARVFSGIQPTGDLHLGNYLGAVVNWVRDLDRDESIFCIVDLHA
                     ITIPQDPAELKARSRNLAALLMACGLDPERCILFVQSHVPQHAQLAWLMQSVVSYGEL
                     GRMTQFKDKSDQHKFVSAGLFTYPALQAADILLYDTEKVPVGADQKQHIELCRDAAER
                     FNGRYGTEEQPIFVMPVHSTPAAGARVMDLQHPDKKMSKSLDSPQGTVLVLDDPKAIE
                     KKFKRAVTDSEGVVRYAPDDPGKAGVTNLLSILAGATGDDPEALASKYEQYGPLKADA
                     AAAVIEQLVPIQERYATLVADPAELDRLLAVGADKAEAIASPVLHRATTAMGLGRG"
     gene            complement(13174..13722)
                     /locus_tag="PKY13_03655"
     CDS             complement(13174..13722)
                     /locus_tag="PKY13_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79053.1"
                     /translation="MKLDLAHRIPRFFGSLRPGPPSATDVSWALAALNEREQRLFTQM
                     SNPDRRHAVRVARRVEAAYAEQFGTGGAAPEVALEVAVAAGLLHDVGKTEARLGTYGR
                     AVATLSGAVGGLDLAEIWQEKSGFTRRVGMYLRYGIIGEQMLELAGSDPVVIAWSVEH
                     HLPADAWTVPTQFGEILTAADE"
     gene            complement(13719..14648)
                     /locus_tag="PKY13_03660"
     CDS             complement(13719..14648)
                     /locus_tag="PKY13_03660"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HOT79054.1"
                     /translation="MTPEADVDAFLTWMAVEKGRARNTLAAYRRDLGRYLAWLGEHGV
                     AVRDATNEDVEAHVEWLRAQGLAPASVIRAVTVVRGLHRFLSAEERTDHDPTLRLETP
                     RRPQSLPKALTEEQIERLFAAVAQADGPVGLRDSALLEVLYGSGLRISEAVGLSMGDI
                     DLDGRLLRAFGKGAKERIVPIGGVAGRALAMWFDARPALFPARWRSRDDEMAVFLNQR
                     GGRLTRQGGWLVLDGHARRAGLADVVSPHVLRHSCATHMLDRGADIRAVQELLGHASI
                     STTQLYTKVVTERLWRVYGESHPRARRPAGARS"
     gene            complement(14645..>15192)
                     /locus_tag="PKY13_03665"
     CDS             complement(14645..>15192)
                     /locus_tag="PKY13_03665"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT79055.1"
                     /translation="HDHPFRKVGDDLIHQGFVISVTNAKFEGPDGVIMDRDVVHHPGA
                     VTVVPVLADGTVVMVRQFRSSIEEMLLELPAGKRDVADEPPAVTAARELREEVGLEAT
                     ELIPLVELLHSPGFCDEVNHIFLATGLTEVERSVDGPEEEHMTIVHLPLADLYDRIAD
                     GTLTDAKTIVGLTLAYHRLLG"
BASE COUNT         2445 a         5444 c         5150 g         2153 t
ORIGIN      
        1 caacaacgcg ttcaccgaca tctgcgagat cgcccgcgac gagggcggca tcgtgaccct
       61 cgccatcaac gccgggttgc cgctccccga cctcgtcatc accctcggcc gtgacggctg
      121 tgtgtcgccg ccggcgttcg agggtccgac cgtgtggccg atcacccgcc acttcgtcac
      181 cgctgcgctc cgcttcaacg ccggactcga ccccgaaccg gtcggtctcg ggagccaggt
      241 cgtcggcgag ttcagcccgg caccgtccac ctacctgcac cggccctgag accggcgggc
      301 gggacaccgc ccgtagactg cgctgatggc tgtcgatcga gtgctgctgg ccgcgccgcg
      361 ggggttctgc gccggcgtgg agatggccat caaagcgttg gcgtggatgg tgcgggcatt
      421 cgaaccgccc gtctactgct accacgagat cgtccacaat cagcgggtcg tgcagcgctt
      481 ccgcgacctc ggggtggtct tcgtcgacga catcgccgag gtgcccgagg gcgccccgat
      541 catgttgtcg gcccatggct cggcccccga ggtggtcgcc gaggcgatgg cccgcggcgg
      601 gtacgtcgtc gattcggtgt gtccgctcgt caccaaggtc caccacgagg tgaaagtgcg
      661 ggccggcaag ggctatcaga tcgtctacgt cggccacgag ggccacgaag aggcggtcgg
      721 caccatggcc gtcgcccccg agtcgacaca ccgcgtcgag tccatcgccg aggtcgcggc
      781 gctcccagcg ttcgagacgc ccgtcgcgct gctcgcccag acgacgctca gccaccgcga
      841 ctggagggac gtcctcgacg ccaccaagga acgcttcccc gacatgtggg tccccgggcg
      901 cagcgacctg tgcttcgcga ccaccaaccg gcagacggcc ctcatgaaca tcgcaccccg
      961 gtgcgacgcg atggtggtcg tcggctccgc caactcgtcc aacacgctcg ccctcgagaa
     1021 gctggcccgc gaggccggat gcccacaggt gttccgcatc aacgacgccg acgaactgcc
     1081 cgacgagctg tcgggaatcg tcggggtcac cgctggggcc tcggcacccg aggacctcgt
     1141 cgaggcggtc atcgcccgtc tggcgccggc gaacggggtc gaggaggtcc gcacgaccga
     1201 cgaggacgag tacttcccgc cgccgcgcaa cctgcgcgac ctgctgctgg ccgtcgactc
     1261 gttcgccacg ttcgcacttg gcggcgaccc cgtcgatcgc cccgggctca acgaccgcaa
     1321 gcttccggcg agcgacgtcc tcagctcgct cgcctgaacc aagggtcgca actgccccac
     1381 gagggcgttc tcgactcacg gtttggcgag cagcgaccgg accacctcca cgacctcgtc
     1441 cggtgagtcg ctgatctggt gatgggtgaa ccgcaccggc cgctgatcgt tcatcaacgc
     1501 cagctgatca cgacgccggt ctcgctcgaa cgcctcgagc cgggcatgga accgtcggcc
     1561 gtccaactcg acgacgaggc gggccgcctc gtaccagaag tcgacccggc ccgcctcgcc
     1621 cggggcccag tcgaaggtca cctgcgattg cgggatggcg aggccggcgc cgacgatcag
     1681 gtcgaggaac atcgtctcga gctccgattc ggttggcggc gtgccgtcga gccggacgag
     1741 gacgtcgcgc atgcgcttcg ttcccggccg gccctgccgg ctcacacggg agaagacccg
     1801 ctcgacgcgg tcgaatgtgg tcaaccgatc gcgcagcgcc gcctcgacga ccgacgagag
     1861 ctggcgaggc ggcagcgtcg caccgagatc gacaagggtg cgctccagcg tggtgaccgg
     1921 gaacccgcga cgtcgcgtga cgtcgatcgc atcgagcgcg gaccggtgca ctgcgacccc
     1981 acgacaccgt cccgtcgagc ccggcgggac gcacacgtgg acgactcggt cgtcgacctc
     2041 gacgccgtgg acccgccccg cggagtggtg ccccagcacc gatcccgaca cggcggcgag
     2101 cgccgctgtc gcaagcatct cgaacgtctc cggcaccccg gcgatcacca gcatcccgca
     2161 gggcaatcga cgccactcgc cggtctgcac acggtggcgg cgttgtttcg ccgacacgcc
     2221 ctcgcggtcc agcacgtcgg gcagcagcac cccgtggtcg cgggtggcgc agcgcatgag
     2281 ccgaagctgg tccatggtct cgattcgtcc ggtcgatgac gggggtgggg ggaaggggac
     2341 ggaccgacag tacgaagcgg gcggtcgccc ggccacgggg gacctccccc attggaaccg
     2401 agggcccgaa gcgccctcca agggcacatt cgaccctcgg cttggcgtcg gcggcccccg
     2461 ctcggcgatg ctggcgccca tggacacctt ccgtctcttc ctccacgtcc tcgcggcctc
     2521 ggtctgggtc ggcggccaga tcgtgctcgg cggtctcgtg ccgacgttgc gcaagatcag
     2581 ccccgaagcg cccaagctgg ccgctcaggc cttcaacagg atcgcgtggc ccgccttcgg
     2641 ggtcgcgctg gtcacgggca tctggaacat gctcgtggtg gaggacctcg atcaggccct
     2701 gttcgggatc aagttcctgc tcgtgatcgt gtcgggcgcc ggcgcggcga tccacatcgt
     2761 cggcaagagc aaggcagctc tggccgtcgg cggcgcgctc gcgtcggtcg gcgccatcgc
     2821 cgccatgtac gtcggcctcg cgctgtagct catcgaccgg ccggcagcac cggaccactg
     2881 acacggcggg cctgatacgc cgccgtcgcg atcgcagcga gcgccccgag gagggcgata
     2941 ccaccgatcg acaccgggtc gaccaccggg cggaggccct cctcgcccat cgacgtgatg
     3001 aagccggccg ccatcgacca tccggtcacc acgccgagcg ccgagccgaa cacggcgatc
     3061 agcagcgtct cgatgacgac cgtccggtgg aactgggacc gagtgagccc gagggcccgg
     3121 agccgagtgg tctcggttcg ccgctcgcgc agcgccagcg cgacggtgtt cgcgaccccg
     3181 acccaggcga ccaacacgct cacccccacc aggaccacga gcaggttgat gagcgagttg
     3241 atacccgtca ttcgttggcc gaggtactcc tggacgctcg tcacgcttgc gccgggggcc
     3301 agtgcccgga gttcgcgccg agcagccgcc accacctcgg gaaccgatgc tcgatcgtcg
     3361 gccttcgccc agacgaccga atctccggag aactcgggtg gtgcgttcga actcgtcgtt
     3421 gcgaggttgg cgacgtactc ggcgtcgaca ccgaaccggc cggtgaaggt cgcagcaagg
     3481 cgcagcgtcg ctcgccccgt cgtcgcgaac cacacctcga cgtcgtcgcc gacgcgccaa
     3541 cccgcccgct ccgcggtctt ggcgagcacc atgacctcgc cgtcatcgag gtgcgtgatc
     3601 ggtggggaga gtcccggcac ctcgatcatc cggctggcag cgtcgaggtc gacgccggtc
     3661 agaaacttcg ccgcaccgat cggcggaaca gcggacgagg cggtcgcggc gtcgaccccg
     3721 gcggcgtcgc gcgcctgcgc cgcgtcgtag ctgttgagca ccgtcatcac gcccagccgg
     3781 accggcgtca ccgcgtcgat gccgtcgagt tcggacagtc gcgccgcgac cgtgcgcggc
     3841 acgccgaccg gtgactcgac caggaggtcg atgcccgcga gttgtccggc agcgcgatcc
     3901 tcggtcgcag cgcgaaccga cgcctcgaac gtggtgatga ccgtcatcat cgcgatgccg
     3961 atgctgagcg ccacgatcac gagcgtcgtc cgtcccggac tccgcgccgc cgatcggatg
     4021 gcgagccgcg ccgtgaggcc atgccacgca accagcgggc gcccgaccca tccgaacacc
     4081 gggcccacca ccgctgagcc gaccatcgca aacgcgatga agacggccgc cgatccgacg
     4141 ccgataccgg cgagaccgag ctcggcggcg cccgtcagga tcaacgagat cagtgtgacc
     4201 cccgcgacga ggagcgccag tccactccag tgccgacggc cccagcggta cgtgtcgtcg
     4261 acctgggtcg aacggagcac cgacatcggc gacgtcgacc ccgcacgtac tgcggggacg
     4321 atgccggcga ccagggtgga accgaggccc gtagcgaatg cagcgatgac cgaaccccag
     4381 ccgaccccga gcgggccgtc gggaaggtcg agggccctgg cgaacaccga ccgagtcgac
     4441 cacgcggcga gccaaccggc ggcaagaccc acaaccgatc cgacgacccc gatcacgact
     4501 gcctcggaca gcaccgtgcg ggtcacctgc gcacgggtcg ccccgatcgc ccgaagcatc
     4561 ccgagttccc gaacccgttg cgccatcacg atcgagaacg tgttgaagat cacgaacgcc
     4621 cccacgaaga cggcgatgaa ccccgacgcc atcatcgggc gatcgaagaa ctccgcaatg
     4681 cgccgcacga cagcctgccg ttcccccacc agcgtcgacc cggtcacgac ctcgacaccg
     4741 gcgctcgagt cgctatcggc actcgaatcg gcgccgagct cgctcccgat cctcgagcgg
     4801 agccgttcga gggccccatc tccgagatcg ccaccggcgc ggccgatcac gaagtcgaag
     4861 cccgacgact tgtcgagggc ggaacgcgta gtcggctcgt ccatccagac cagtcgaggg
     4921 ccgggcacga tctcgccgtc gcggacgatg gccgccacgc cgacgaccgt caaccgccga
     4981 gatgcaaccg ggaagtcgac ccggacctcg tcgcccggca cgacgccggc gacggcggcc
     5041 gtgccggtgt ccagcacgac ctcgccgtcg gccacaggag ctcgcccctc gacgatctcg
     5101 acgccggcga gctcgggatc caccagccag ttcgtggcga cggttccggc cccgaagagc
     5161 tcgtcgatcg gacgaccgtc tcgaccgagc acggtcgcag cgccctcgat cacaccggca
     5221 acggcgacga ggtcacccga agcggccatg gccgacgcaa cgtcaccggg caccgccgcc
     5281 cgaagcgccg ccccgccgat ctcctgcccg ccaccagcac ggaggaccac gtcgacaccg
     5341 ccgaatcgcc cgtcgagcgc gccgtcgagc gtcgaccgcg tgagtccacc gagcgcgagg
     5401 gtggccgtca cgaaggccac cgacacgacg acggccgcca gcagaccgaa ccatcgttgt
     5461 cgctcgaccc agaccgcacg aaccggcttc agcacgcgcg atcaccacat tgcgtcgccc
     5521 cagcagccat cgtcctgatc ttggaccggc gtcagcccga atccgccgcg gcgcacacaa
     5581 tcttcacatg tcggccggcg agccccgacc tcccgcggat cagccggcga gccgccgggc
     5641 ttcgtcgtag accccctgca cctcgccacg cagctgctcg gtcagggccg agatcgccga
     5701 acggggcaca cgtccctcga tgtcgacatc ggggtagatc ggctcgccga tcacgatcac
     5761 gaccttgtgg ggcaggatcc acttcttgcc gatcggcatc gccttgtcgg agccgccgat
     5821 gccgatcggg atgatcggca cccgctgccg tgccgcgacg aacgccgggc cgtcgaacag
     5881 gtcggcgacg tcgtcgcccg acttccggcg cccctcggga aacacgacca ccggctcccc
     5941 tgccgccagg aggtcctcgc acgtccggat cgcgctgaag tcggcggtgt cacgcaccac
     6001 cgggaaggcg cccatcacgt tgaggaaccg gtcgacgagc ccaccgagga agatcgacga
     6061 cttggccatg taccggacgg ggcgcttcac cgcgagcccg gcgaggagga agtcgaggtt
     6121 cgagcggtgc acgggagcga ccacgaacgc accggatcga ggcacccgct cgcccccgat
     6181 cacttcgagc cgccagtacg cgccggtgag tccgcgggcg agagcccaca acacccggta
     6241 ggcggcgagg gcgacgggac cgcgtgggga tggtttcatg agggttcctt tcgtgcgacc
     6301 cgcccgaggc cgcgggcctc ggcttcggcc acgatgcgat cgacgacctg ctcgatcgtg
     6361 aggtcggagg tatcgacgat gacggcgtca tcggcgggtc ggaggggcga gtcggcgcgg
     6421 gtcgaatcga tccggtcgcg ctcggcgatg ttggcggcgg cggcctcgag gtcgaagtcg
     6481 ccgagatcgt cgccggcacg gcggcgggcc cgcacttccg gcgacgccgt caggtagatc
     6541 ttcaaggggg cgtcggggag caccacggtg ccgatgtcgc ggccttcgag caccccgccg
     6601 ccgcgtcggg ccatccactc gcgctggagg ccccgcagcc gacggcggac gccgggatgt
     6661 gtggcgacga tgctgacgtt ggccgacacc tcgggcccac ggatctcggt ggtgacgtcg
     6721 tcaccgttga tcgtcacccg ttcgtcgacc acgatctcgg cgcgttcggc cgacgccgtg
     6781 gccccctccg cgtcgtgcac gtcgccgccg gcacggagga ccgcgcacgt gacggcgcgg
     6841 tacatcgccc cggtgtcgag cattggcagg ccgacccggt gggcgaccat cttcgagatc
     6901 gtcgacttac ccgatccggc cgggccgtcg atcgcgatga cgttgccgat gtcagcgttc
     6961 tcgttcaccg aggcgctcct ctcaacagtt cgagcgttgc ccagaagccc ggaaaggtct
     7021 tggccacgca accggggtcg gcgatcgcga tgccggggac cttcaggccg aggatcgcga
     7081 agctcatcgc catccgatgg tcgtcgtagg tctgcaccgt gcccggagac accgggccgg
     7141 gctcgatcac gaacccgtca tcgagttcgg tggccccgat gccgaggcgc tgcagctccg
     7201 tcacgacggc gtggatccgg tccgtctcct tgcggcggat gaacccgatg ccctcgacgg
     7261 tcaccgaggc gctcgcgaac gggacgatcg ccgccagcgt ctgcgcggtg tcggagatgt
     7321 ccgccaggtc gaacactccg cctgacaacg ccccaccggt gaccgtgacc gagccgggcc
     7381 ggagttcgac gtcggcgccg cacgaccgca gcacctcggc gaagcggacg tcgccctgca
     7441 ggctgtccga tccgagcccg tcgatcgtga ccgacccgcc gcacagggcc gccgccgcca
     7501 ggaagtagga cgccgccgac gcgtcgggct cgatgtcgta ggagatctgt tggtagccgg
     7561 tgggttcgac cgcgaaccag cgacgatcgt cggcgacctc gaccttggcg ccgaacgccc
     7621 gcatcacggc gacggtcagg tcgacgtacg gcaccgactg cagtgggccg tcgacggtga
     7681 tctccagccc gtcgggcagg cagggggcga tcaggagcag gcccgacagg aactggctcg
     7741 acacgtcgcc acgtacctcg accgatccgg cgagctcgtc gacgtcggga tcgggcgcga
     7801 cggtgaccgg caagcacccg tcgttcgacg tcacgctccg gccgagcgct tcgatcgccg
     7861 caatgccgtc ggccatcggg cgggcccgca tggactcgtg tccgtcgagc gtgatcgagt
     7921 ccttgccgag cgcggccagt ggcagcagga accgggacgt cgtgcccgac atccggacgt
     7981 cgagcgtggc ggagcgcacg ggtagccgac cgctacagcc ccacacgctg gcacggtcgc
     8041 cgtcgacctc gatctggatg cccagcgttc gcagcgcctc gagcatggcg ttggtgtcgt
     8101 cggagtcgag gatgccggtg aggaccgatg gcccgttgcc gagcgccgcg cagacaaggg
     8161 cgcggttcgt gatcgacttc gagccgggga tcggcaccgt gacatcgggg atcggccccg
     8221 aaagcggctc gatcggcaac gggtcgggca gcgacgcgag atcgttccag tcgatgccga
     8281 tcacgtgagc gctcgctgcg acaccgcgta cccgtcggcg aacaggctct cgacgaacgt
     8341 gtcgaccttg ccggagtcga cgaggaggat gagcacaccc cgcggcccct ccgccgagtg
     8401 cgcgatttcg atgtcgtaga tgttgacctc atgctgcgtc gccatggccg tgacggcggc
     8461 cagcacgccg ggacggtcgg gcacgccgac gcgcagctcc accagttcgt cggggcgggt
     8521 cgccgtcgtc ggcaggttca ggcgaccctc gcgagcggac tccagccgat cgatcagcgc
     8581 gttgcggtcg ttcccgtcga cgatcgaccg aacctcggtg agctcacgga tgagatcgtc
     8641 gagcacgtcg acgatcgcct cgcggttgtc gccacagata tcgggccaga tgtaggggct
     8701 gcccgacgcg atgcgggtca tgtcgcgaaa tccaccggcg gcgagccgaa ggagtgcacc
     8761 acgaccttcc tgggtcgatc gctggcctgc gaggcccatc aacgtcgccg ccgtcaggtg
     8821 cggcacgtgg gacaccatgg cgacgaactg gtcgtggacg gcagcatcga gggtgatcac
     8881 gtcggctccg aacgacgcga cgaccgagtg caccatcgcc tgcgccttgg gatcggtggt
     8941 ggcggtcggg gtcaggaccc acacggcccc ctcgaacatc gaggcgctcg agccctggat
     9001 gccgtcctgc tctgacccgg ccatcgggtg gccgcccacg aatctcgggc tgtcgagggc
     9061 agccacgatc gggcccttca cgctcccgac gtcggtgact acccaatcgg tctcctccag
     9121 cacccgacgg ccgtcggagg aaatcccgct cacgggcatc gccaggaagc agatcgaggc
     9181 gcggtcgtcg agcccaatct cgtcgatcgc tccccgttcc cgtgcgtgct gcgcgttgaa
     9241 gttgctctga tcgtgccccg tcacgtgcca gcgacgggcg cggagcgcca tcccgatcga
     9301 gccgccgatc agaccggtac cgatcacctg ggcgcgccgc tgctcgctca tcggatgccg
     9361 ttcactcggg caggtcgtcg cgcaggctgc gggcgtcgtg gaggtacacg tgacgcagct
     9421 cgtcgcgacc cagttcggtc tcgaggtgca tcatgacccg cacgcaccgg ggcatcgccc
     9481 cgtcgatcga cagctcgcgg gcgcagatga gcggaacgtc gcccaggccg accttgcgcg
     9541 ctgccgtcgc cgggaacgtg ctcgtgaggt cctcggtcgc cgtgaagatg atcgagatca
     9601 gtgcgtcgtg ggcgatgccg ttgcgttcga gcatctcggt gacgagctcg accacacggt
     9661 cggagaggtg gtcggcgtcg tcgacgtcga cggtggtggc cccacgcagg gcccgaacgc
     9721 tggtgggcat gggacgagat gctacctgtc ccccaaccac ccgctactcg gcggccccgg
     9781 cagcgcgttc gagcgaccgc acctcctgct gggtcagctc ccgccacgcc cccggcgcca
     9841 acgtcgtgtc gctcagcggg ccgatccggg tgcgaaccag tcggaccacc ggatgtccga
     9901 cggcgtcgca catacgccgc acctgacggt tgcgaccttc gtggatgacg aggcggatca
     9961 ggttgggagc gagcagcgcc gcagtggccg gtgcagtgga gccgtcctcc agctcgacgc
    10021 cctcgcgaag tcgccgcagg gcgccccgcg acggttcgcc ctcgacctcg gccaggtact
    10081 ccttgtcgac gccgaagctc ggatgggtga gccgatgggt caggtcaccg tcgttcgtga
    10141 ggagcagcag gccctcggtg tcggcgtcga ggcggcccac cgggaacacc cggggctcgg
    10201 ccggcaccag ctcgacgacg gtcggacgcc catgggtgtc ctgcgcagtg gtcaccacac
    10261 cggccggctt gttgagcagg tagtggacga ggcccggttt caccccgatc tggctgccgt
    10321 cgaactcgac gacgtcgacg agcggatcga cccgctggcc gaggtgggcg atgaccccgt
    10381 tgaccgagat gcggccctcg tcgatgtaga tctcacacac ccgacggctc ccgatgccgg
    10441 cctgggcgag gaccttctgc aggcgctcgc cctcgggcag accgccgggc tcgccgctca
    10501 atcgtccgcg aggacgtcga gggcgtcgag cgagagcggc gcctcgacga cgggggcctc
    10561 agtgaccggc gcctcagtaa caggcgtctg atcgcggagg tcgatgaccg gctcatcggc
    10621 ctcgtgaacc gccgcctcgt gagccgccgc ttcgtgggcg gccgactggg ccggggcgtc
    10681 gtgcgccgaa tcctcgttga cccgcaggcc cgcttcgagc gcctcgacga cgtcggggcc
    10741 gggcacgaag tcgccgagcg gcgggagctc gtcgaggctg ttgagcccga ggcgctcgag
    10801 gaacagcgat gtcgtgccga acagcaccgc ctggccgggc cccggatcgc gggccagttc
    10861 gccgatgtag ccgcgctgct gcagggtccg catcacaccg tcgacgctga caccacggat
    10921 cgcagcgacc tgggcgcgag agatcggctg cttgtacgcg acgatcgaca gcgtttcgag
    10981 cgcagcggcc gacaggcggg ccgactggcc ggtgagcaca aagcgctcga cgtacggcga
    11041 caactcggca cggctctgga accgccagcc gccggccacc ttcacgaggg caaacccccg
    11101 ctgttgggcg tcgtactcgg ccgcgagttc gacacagagc tcgtcgactc gagcggcggg
    11161 gatctcgatc agctgcgcca ggaggcgagg atcggcgggt tcatcggaca ccatgacgat
    11221 ggcttcgagg gcgcgcttgg cctcggccgc cagcggcgat cggtgctcga tcggttcgga
    11281 ctcggacatc ggctatccct cgtacatgtc gacgagcgcc aggtcggcgc cgtctcccag
    11341 ctcaccaccc gtccactcga tcgtgatgtc gccgaagttc gtcggctggt cgagatcgat
    11401 gtacccctgc ttgaacagtt cgaggacggc gaggaagcgt acgacgatct cgagcttctc
    11461 cacgaatgcg gccgtgaggt ctcggaacga gatgcggccg atccggggca actcgtcgat
    11521 gagctcggcg accgcctcga cgaccgacgc acggatcggg gcgacgtgga acaggtcgat
    11581 gcggggctcg ggcttgggcg ccgtcgcctt catgaacgcc gaccggagct gcaacggggt
    11641 cacgccgacc agcgagtcgg gcgtgaggtc gatgaaccgc tcgtcggggc ccacggtgcg
    11701 ggcgaagcac tggccggcgc tggcgtgaag gtcggccagc acgatcgcgg cgtccttgaa
    11761 cgtcttgcac tcgagcaggc gggccagcag gagatcgcgt tcttcccaga tacccagttc
    11821 gtcgtcgaga tcgccgtcgt cgagaccggg gagcagccgg cgggccttga gctcgaccag
    11881 cgtggcagcg atcaggagga actcggtggc gacgtcgaga tccatctccg acatctcgga
    11941 gagatgggtg aggtaggcgt cgacgatctt ggtgagcgac acctcgtaga ggtcgacctg
    12001 gtcgcggagg atcaggtgca gcagcaggtc gaacggcccc tggaacacgt cggtctgcac
    12061 gtcgatcgcc acgagcggca tcctacaacc ggcctcccgt cgaattacaa cagggtgatt
    12121 cagcgaccgc tcccctaacc tgtccccatg gcacgcgtct tctccggcat ccaaccgacc
    12181 ggcgacctcc acctgggcaa ctacctcgga gcggtcgtca actgggtgcg cgacctcgac
    12241 cgggacgagt cgatcttctg catcgtcgac ctgcacgcca tcacgatccc ccaggatccg
    12301 gcggagctga aggcacgcag ccgcaacctc gccgcgctgc tcatggcctg tggcctcgac
    12361 cccgagcggt gcatcctgtt cgtgcagtca cacgtgcccc agcacgccca gctcgcctgg
    12421 ctgatgcaga gcgtcgtctc ctacggggag ctcggccgca tgacccagtt caaggacaag
    12481 agcgatcagc acaagttcgt gtcggccggc ctgttcacct acccggccct gcaggcggcg
    12541 gacatcctcc tctacgacac cgagaaggtc cccgtcggcg ccgaccagaa gcagcacatc
    12601 gagctgtgcc gcgacgccgc cgagcggttc aacggccgct acggcaccga ggagcagccg
    12661 atcttcgtga tgccggtgca ctcgaccccc gctgccggcg cccgggtgat ggatctccag
    12721 caccccgaca agaagatgtc gaagtcgctc gactccccgc agggcaccgt gctcgtgctc
    12781 gacgacccca aggcgatcga gaagaagttc aagcgtgccg tcaccgactc cgagggtgtg
    12841 gtgcggtacg caccggacga tcccggcaag gccggcgtca ccaacctgct gtcgatcctc
    12901 gccggcgcca ccggcgacga ccccgaggcg ttggcctcga agtacgagca gtacggcccc
    12961 ctcaaggctg acgccgccgc tgcggtgatc gaacaactcg tcccgatcca ggagcgctac
    13021 gccaccctgg tggccgaccc cgccgagctg gaccgtctcc tcgccgtcgg cgccgacaag
    13081 gccgaggcga tcgcttcacc cgtcctccac cgggccacca cggcgatggg cctcggccgg
    13141 ggctgacctc gcccgacgtc gggcgaccac cgcctactcg tcggcagcgg tgaggatctc
    13201 gccgaactgc gtgggcacgg tccaggcgtc cgcgggcagg tggtgctcga cggaccaggc
    13261 gatgaccacc ggatcgcttc cggcgagctc cagcatctgc tcgccgatga tgccgtagcg
    13321 caggtacatg ccgacccgac gcgtgaatcc cgacttctcc tgccagatct cggccagatc
    13381 gagtccaccg accgcacccg agagggtcgc aacggcgcgg ccataggtgc cgagacgggc
    13441 ctcggtcttg ccgacgtcgt gcaacaagcc ggcggcgacc gcaacctcga gggcgacctc
    13501 gggggcggcc ccaccggtcc cgaactgctc ggcgtacgcc gcttcgaccc ggcgggcgac
    13561 acgcacggcg tgtcggcgat cggggttcga catctgggtg aacaggcgct gttcccgttc
    13621 gttgagcgcc gcgagtgccc acgacacgtc cgtcgccgac ggcgggccgg gacgcagcga
    13681 cccgaagaag cgcgggatcc ggtgggcgag gtcgagcttc acgaccgcgc tccggccgga
    13741 cgccgcgccc gggggtggct ctcgccgtag acacgccaca accgttcggt gacgaccttc
    13801 gtgtacagct gggtcgtcga gatcgaggcg tggccgagga gctcctgcac cgctctgatg
    13861 tcggcacccc ggtcgagcat gtgagtggcg cacgaatggc gcagcacgtg cggtgagacg
    13921 acgtcggcca ggccggcccg gcgggcatgg ccgtccagca cgagccagcc gccctgacgg
    13981 gtcaagcgcc cgccgcgctg gttcaggaac accgccatct cgtcgtcgcg gctgcgccac
    14041 cgcgccggga acaacgccgg ccgggcgtcg aaccacatcg ccagcgcacg accggccaca
    14101 ccgccgatcg gcacgatccg ctccttggcc cccttgccga aggcccgcag cagccggccg
    14161 tcgaggtcga tgtcgcccat cgacagcccg acggcctcgg agatccgcag accggagccg
    14221 tacagcacct cgagcagcgc ggagtcgcgg agcccgacgg ggccgtcggc ctgcgccacc
    14281 gcggcgaaga gtcgctcgat ctgctcctcg gtcaacgcct tgggaaggct ctgcggccga
    14341 cgcggtgtct cgagacgcaa cgtcggatcg tgatcggtcc gctcctcggc ggacaggaac
    14401 cggtgcaggc cccgcaccac cgtgactgct cggatgaccg acgccggcgc caggccctgt
    14461 gcccgcaacc actcgacgtg agcctccacg tcctcgttcg tggcgtcgcg gaccgcaacg
    14521 ccgtgctcgc ccagccaggc gagatatcgg cccaggtccc gccggtaggc ggcgagtgtg
    14581 ttgcgcgccc ggcccttctc gacggccatc caggtcagga aggcgtcgac gtcggcctcg
    14641 ggggtcatcc gagcaggcgg tggtacgcca acgtgagccc gacgatcgtc ttcgcgtcgg
    14701 tgagcgtgcc gtcggcgatg cggtcgtaca ggtcggcgag cggcaggtgc acgatcgtca
    14761 tgtgctcctc ctcggggccg tcgaccgatc gttcgacctc ggtgagcccg gtggcgagga
    14821 agatgtggtt gacctcgtcg cagaaccccg gcgagtgcag cagctcgacg agcggaatga
    14881 gttcggtggc ctcgaggccc acctcttcac gcaactcgcg tgccgcggtc acggcgggcg
    14941 gctcgtcggc cacgtcgcgc ttgccggccg ggagctcgag gagcatctcc tcgatcgacg
    15001 aacggaactg ccggaccatc accacggtcc cgtcggccag caccggcacc acggtcaccg
    15061 ccccgggatg gtggacgacg tcgcgatcca tgatcacccc gtcgggcccc tcgaacttcg
    15121 cgttggtgac gctgatgacg aacccctgat ggatcaggtc gtcgccgacc ttgcgaaagg
    15181 ggtgatcgtg ga
//
LOCUS       DAOSOA010000031        15156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3945_length_15156_cov_6.535991, whole genome shotgun sequence.
ACCESSION   DAOSOA010000031 DAOSOA010000000
VERSION     DAOSOA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15156
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3945_length_15156_cov_6.535991"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKY13_03670"
     CDS             complement(<1..333)
                     /locus_tag="PKY13_03670"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOT79056.1"
                     /translation="MSGEAGVETSGTDVGDATVPRSDPSGDASSDAHDTTIVALPRPS
                     AVRNVRRFIGCPTAGTLPGCGAHGVCRLRSAVPFSRRQQMAATIVVGTQWGDEGKGKF
                     TDLIAKEMT"
     gene            350..1756
                     /locus_tag="PKY13_03675"
     CDS             350..1756
                     /locus_tag="PKY13_03675"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOT79057.1"
                     /translation="MGVASGDPTPTSVILWTRLLTELTEPVDVKWEVATDAEFTEPVG
                     AGIVPATPELGHSIHLDATDLEPDTWYWYRFSSAQYTSPVGRTRTMPADDATPDELRL
                     GFASCQDFTEGYYPAHRAMAADQLDLVFFLGDYIYESASNDTSVRQVPGGECMTLADY
                     RLRYATYTGDADLQASRASCPWVVTWDDHEVENNYADDANDGGLDTAGFQDRRAAAYL
                     AYYEHMPVRLDPPDGPDWRIYRSVRFGSLAEFFVLDGRQYRTDQACNKRNDAIVPLSS
                     CPELGDEDRTMLGTEQESWLSDGLAAAETTWKVLAQQTVMSSLVIGDLVLNVDQWDGY
                     PAARQRLLQFIADNNISDVVVLTGDIHSAAGGNLAPASDDGSRSPVAVEFVGTSITSG
                     SLVDIVPGGAALVTADKFPGVAYINVRDHGYGRCTVTPGEWTTEFVVVESVKTADAAA
                     RVDATCVTKQGEPVVTKL"
     gene            1808..2545
                     /locus_tag="PKY13_03680"
     CDS             1808..2545
                     /locus_tag="PKY13_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT79058.1"
                     /translation="MSNATKAIIGAVVAVAVLALGGTWLYINVIKDDAPEKLSLDDTA
                     SGSDATTTTAASGDAPTTTSGEAPAAGDGVDGEWTATDASVVGYRAKEVLFGQDTEGV
                     GRTNAVSGTLTIAGTSVESASFEVDMTSLKSDESNRDNQFNGRIMETSTYPTATFELT
                     SPVDLGSVPEDGVEITATATGQLTLKDQTKDVDLDITAKREGSTISVLTSYSVKFADW
                     NIDDPSFGPAQVEDNALLEIKLVFERA"
     gene            complement(2573..2758)
                     /locus_tag="PKY13_03685"
     CDS             complement(2573..2758)
                     /locus_tag="PKY13_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79059.1"
                     /translation="MAGTEINPVPTDEEAVAIVAAIEGAWPRGAAEPSRDDSPRWRWS
                     GRWWTKPMISRRDRPWM"
     gene            complement(2758..4293)
                     /locus_tag="PKY13_03690"
     CDS             complement(2758..4293)
                     /locus_tag="PKY13_03690"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT79060.1"
                     /translation="MSERVEDLRRRKQEALTPGSERSIERQHSRGKMLARERIDYFLD
                     EGSFHELDGLARSRNAAATERPYTDGVITGWGTVDGRQVFVFSQDFTVFGGALGEVYA
                     EKIHKVMDMALKVGAPVIGLNDGAGARIQEGVVSLASYGGIFYRNVLASGVTPQISVI
                     MGPCAGGSVYSPIMTDFIFMVDQSSYMFITGPDVVKQVTGEDVSQQDLGGARVHTSKS
                     GVAQFVSGDDKAALDDVRYLLSFLPANNLEEAPRLETDDPSDRLCPELEQLIPASANQ
                     AYDMRKVISTVADDGEFFEYASAWAPNIVCGFARLGGQSVGIVGNQPMAMAGVLDIES
                     SSKAAKFVRTCDAFNIPLVTFVDVPGFLPGVDQEHNGIIRHGAKLLYAYCEATVPRIS
                     VITRKAYGGAYVVMDSKSIGSDLAFAWPSAELAVMGASGAVEIVNRRELAEADDPVAR
                     RAELIDAYTEQFCNPWEAADRGYVDDVIDPAETRLKLIAGLTMLRSKKESLPPRKHGN
                     MPL"
     gene            4362..>4906
                     /locus_tag="PKY13_03695"
     CDS             4362..>4906
                     /locus_tag="PKY13_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOT79061.1"
                     /translation="MTSPIPAAATADSRLPICVYLASRHGANPSYTEETRRLGAALAA
                     AGCDLVYGGGSVGLMGEVADAVLAGGGAVTGVITEHLYNLEVGHQQVTELIVVPDMPT
                     RKRAMFERAEGFVIAPGGVGTMEELFEVWCWAALGLHPKTLGMLNCDGYYDHLLAFMQ
                     RSVDEGLMSQHTLDLLVVDDDP"
     assembly_gap    4907..5006
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5007..6026
                     /locus_tag="PKY13_03700"
     CDS             <5007..6026
                     /locus_tag="PKY13_03700"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOT79062.1"
                     /translation="PVGDILFEPKWDGFRCIVFRDGDEVILGSRNDKPLTRYFPEMLD
                     PIRSMLPDRCVVDGELVVATDKGLDFDVLGQRIHPAESRINRLAAETPASFVAFDLIA
                     LGDDDLRETPFGERRRLLEGVFADVVPPLHLTPITPDRDLAADWFQRFEGAGFDGVMA
                     KPVADPYLSNKRSILKIKHQRTADMVVAGYRVHKDGNGVGSLLLGLHNDDGQLQHIGV
                     ASSFSAARRVELLDELAPYADRALDDHPWAAWGNARAHEESRMPGAPSRWSGGKDQSW
                     VPLRCELVAEVGYQGLTSGRLRHPAKFLRWRPDKDADQCRYDQLDQAVPIELEQLFGD
                     DRFSN"
     gene            6111..7472
                     /locus_tag="PKY13_03705"
     CDS             6111..7472
                     /locus_tag="PKY13_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT79063.1"
                     /translation="MNTGRRTLAALIAALSITAVAACSEADTPEAASTTAVPTTRGSG
                     APEPKAGDGSVVSKFAGSPWLLGDVPERATPADSSKPPIKVGFMNIDSGPIAATPELH
                     QATDAAIAFINAELGGVGGRPIEIVFCETDLGVDKAQACARLMVEQQVVAVLGGLNLS
                     AGAATKILEENDIAWVGGIPLDPAEMASPVAFQFSGGAPGAFVAFADHAAHELGAKRV
                     AVLYANTPQISVAAVDYGIAMLKALDVDVTEIAFDLTTQDYASIVQRAAEASPDAMLV
                     GAADFACPKVMQAIADLALDTTIYMVGSCADAKWIEQVGAEKIAGTIFNIESRVERTG
                     PYDADIEIYTSVIDQYGKGVNAKGAATVAFRGAMNLYAVLTDLGDDPTSAEVIDAFRG
                     AKERKSFDGYPYTCDGKQIPELPSLCASQEVLVSIAADGQTFSEESDGWIDVPKLVAE
                     YLR"
     gene            7474..9465
                     /locus_tag="PKY13_03710"
     CDS             7474..9465
                     /locus_tag="PKY13_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT79064.1"
                     /translation="MTQHLAYLILGLGAGAALSLLACGVVLTERASGMVNFAHAAMGM
                     YLAHAFYEFRETGDLVLPIVGLPARVHLLPRPTVATAVVVMVVYAAFLGAAVYLLVFR
                     RLAKAPALARVVASIGLFLYLWAMVGLRFPNPPAIRRILPSRAVQILGHPVFQDRLWS
                     AVVAVVVTALLWATFRFARFGLATIAASESRKGAELTGINSSAVALVNWMLATVLAGV
                     AIVLIAPVNQLDPLNLSLLIVPTLAACLLGGSRSFVTVSAAAFAIGMAQSEIVNLQVG
                     WHSLSGLGLQQGLPFLLILATLVVRGRSLPSRGEPEAARLPASPEPRLVAPTALVIGA
                     AAILVMLVAGPSWRSAVITSAIAVTISLSVVVLTGYVGQISLAPFAFAGIAAFGLARL
                     SDWGIPFPLAPLLAIAGTGLIGIAVGVPATKVRGMNLAIATLGAAVAIEQLLFKWRPF
                     VDAGVNGATEPSLFGVNLGISARGSDFPRSAFGIFTVVVATAAAVGIANLRRGRSGVA
                     WLAVRSNERAAAAAGIDVVGAKLSAFALSSLLAGLGGVLLTYQRLTLTTDSFAVFLSL
                     ATLALTYLAGIASVSGAVLAGVLTPLGVFAVISGQDPVRVSPYSFVVNGALLVGVALV
                     YPDGLTGAATRLARGVARRWGSTGRGETSELRGNVAGVE"
     gene            complement(9409..10347)
                     /locus_tag="PKY13_03715"
     CDS             complement(9409..10347)
                     /locus_tag="PKY13_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOT79065.1"
                     /translation="MTDPSPDASPSPSSHAASPSGTQVHLVDGTYELFRYFYSPGGGH
                     LNGSGMEVGAVRGVLRSLAGMLDDGATHIGVATDHVIESFRNDLYAGYKNGDGIDPVL
                     KAQFPLLEDALRSAGFTVWAMVEVEADDALGAAARVAAADPSIERAVICTPDKDLAQC
                     VSDPDIVQFDRRAGVFRDEAGVREKFGVEPESIPDWLGLVGDTADGFPGLAGWGAKSA
                     AAVLSRYRHIEDIPLNPGQWDITVRGGAKLCATLAEHFEDALLFRRIATLEFDVEVGT
                     PADWRWTGPDAGFAEMCALLDAGDVAAKLARLAAAR"
     gene            complement(10387..10872)
                     /locus_tag="PKY13_03720"
     CDS             complement(10387..10872)
                     /locus_tag="PKY13_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT79066.1"
                     /translation="MEPVRWLEADEQLAWRAVVEFCGRIIDGMDTDVAAFGLTGADYE
                     VLVHLSEAEESGLRMSELAARVLVSRSRLTYRVDRLEERGLVVRENCETDRRGFIARL
                     TPAGRQLLVEVAPHHVAGVRARLVDRVDREEFLTLGRIVTKVLGYDLDAEPRQPVGSP
                     A"
     gene            complement(10941..11345)
                     /gene="mce"
                     /locus_tag="PKY13_03725"
     CDS             complement(10941..11345)
                     /gene="mce"
                     /locus_tag="PKY13_03725"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOT79067.1"
                     /translation="MLLTNIDHVAIAVRDLDAAIAYYAEAFGAEVHHREIVESDGVEE
                     ALVKVADSYIQLTAATRPDSPIAKSIEKRGEGLHHIGYRVDDCAEALASMIAAGATPI
                     DTAPRPGSRGTTVAFIHPKGSFGTLIELVQEG"
     gene            complement(11417..12025)
                     /locus_tag="PKY13_03730"
     CDS             complement(11417..12025)
                     /locus_tag="PKY13_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT79068.1"
                     /translation="MDRPPRRLDAVVFDLDGVIRHYDRVHERDIEERHGIPVGSLVPT
                     AFGSSAGHEFMCGRLDHDGFAAALSALIGSEAAAVEFVAMRAEVDAEAVALVRSVQAV
                     VPVALLTNGSLRTRTELAEAGLHEAFDHVFNSAETGIPKPLPESYLNVVAALDVDPTT
                     TAFVDDLDVNVHGAITAGLVGHVYTGLAVLRSFLDVHGIVAS"
     gene            12132..13400
                     /locus_tag="PKY13_03735"
     CDS             12132..13400
                     /locus_tag="PKY13_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT79069.1"
                     /translation="MSDVTPPSGDLPPPPPGPPGPGFWQQSSTSLHEPAPERPPTYVT
                     PPGLQQPLGAAPGPSVPEVAGFWRRAWARGITFLFWLLVLDVGTYLIALLFLFVYSAT
                     PNDSILLAGLAVSWAYGLLVTYLIWSRSLATRRGTLGMRQMGLSIRSASRQPAISKGQ
                     AFRRSIIALLPFVFASGAGWLLLYTPDDDLIVLSLIVQAALVGLVTLGGLWMLVSPRR
                     QTVWDIVGDTVVVVDREPSWLAVSALVAGLLVPTGSIVSLVVVGAARLQSEYRSLSGY
                     GSAGRYFLYNIPTLVLSVIAIALGHVAIRATRWEAQRLAGRGLARTALFLGYLFPVAF
                     VATLAFGAIYRQVEDRQVRSCSDTRDDIAVAAESYRLLNGVYPDSITPMADGIYLDDP
                     ELAERWTLSDESASGVPEYRLTGKGDCAKA"
     gene            complement(13404..14762)
                     /gene="ccrA"
                     /locus_tag="PKY13_03740"
     CDS             complement(13404..14762)
                     /gene="ccrA"
                     /locus_tag="PKY13_03740"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HOT79070.1"
                     /translation="MSMQHILEAIQSDASGDEIANLPLPESFRAAYVLREEQDMWAGF
                     ASEDKDPRKSLHVGEVATPEIAPDEVYIAVMASSINFNTVWTSIFEPLSTFNFLDRLG
                     KESEWGARHKQDFHVVGSDASAVVLRVGSAVRNWKPGDHVTVHCNYLDDQDPSAHDDS
                     MLASNQRIWGFETNYGGLADLSIVKANQLMPKPAHLSWEEAAVNALCASTSYRMLVGS
                     HAARMKQGDNVFVWGATGGIGAYAIQLILNGGGTPVGVVSSEHRVKLLEAMGCENTID
                     RRAENYQFWSDEHTQDEGEWRRLGKKVRSLIGDDPDIVFEHPGRSTMGASVFIAKRGG
                     TVVTCAATSGYMLEFDNRHLWMKLKRIVSSHFANYQEAWNMNQLIGQGRIQPVMSEVY
                     TLDQVGDAALKVHKNQAEGKLGVLCLAPEAGQGITNPERRAEIGEDKITLFARHARGE
                     LA"
     gene            14794..>15156
                     /locus_tag="PKY13_03745"
     CDS             14794..>15156
                     /locus_tag="PKY13_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79071.1"
                     /translation="MARRRGTGATVAIVALASIGLVAGCGDDRPTSTRSPTTVVDRAP
                     RTRPTTTLPDLTASDGLAASIRQFREDEAAGVIQIEMVREPGGEVLVAVTSVRLDWPG
                     FEAVPPTVRPVQVAPGQRV"
BASE COUNT         2314 a         5390 c         4961 g         2391 t
ORIGIN      
        1 ggtcatctcc ttggcgatga ggtcggtgaa cttccccttg ccctcgtcgc cccactgggt
       61 cccgacaacg atcgtcgcgg ccatctgttg cctcctagaa aacggaacag ccgagcgtag
      121 cctgcacact ccgtgggccc cgcagccggg tagcgtgccc gccgtggggc aaccgatgaa
      181 ccgacgaacg ttcctgaccg cgctcggcct cgggagcgcg acgatcgtcg tgtcgtgcgc
      241 gtccgacgac gcgtcaccgg acgggtccga acgtggcacc gtcgcatcgc ccacgtcggt
      301 gcccgacgtc tcgacgccgg cctcgccgct cacgaccgac ccgttcggcg tgggcgtcgc
      361 ctcgggcgac ccgaccccga cctcggtgat cctctggacc cgcctcctca ccgaactcac
      421 cgaaccggtg gacgtgaaat gggaggtggc caccgacgcc gagttcaccg aaccggtcgg
      481 cgccggaatc gtgcccgcca cccccgagct ggggcactcg atccacctcg acgccaccga
      541 cctggagccc gacacctggt actggtaccg gttcagctcc gcgcagtaca cgtccccggt
      601 cggccgcacc cgcacgatgc cggccgacga tgcgacgccc gacgagcttc ggctggggtt
      661 cgcctcgtgt caggacttca ccgagggcta ctacccggcg caccgggcga tggcagccga
      721 tcaactcgac ctcgtgttct tcctcggcga ctacatctac gaatccgcgt ccaacgacac
      781 gtcggtgcgt caggttcccg gcggcgagtg catgacgctc gccgactatc ggctccggta
      841 cgccacgtac acgggcgacg ccgacctgca ggcgagccgc gcatcgtgcc cgtgggtcgt
      901 gacctgggac gaccatgagg tcgagaacaa ctacgccgac gacgccaacg acggcgggct
      961 cgacaccgcc gggttccagg accggcgcgc tgccgcgtac ctcgcctact acgagcacat
     1021 gcccgttcgg ctcgatcctc ccgacggtcc cgactggcgc atctaccgct ccgtccgctt
     1081 cggatcactc gccgagttct tcgtgctcga cggccggcag taccgcaccg atcaggcctg
     1141 caacaagcgc aacgacgcga tcgtgccgct ctcctcgtgc cccgaactcg gcgacgagga
     1201 ccgcaccatg ctgggcaccg agcaggagtc gtggctgtcc gacggcctcg ccgcagccga
     1261 gacgacgtgg aaggtcctgg cccagcagac cgtcatgtcg tcgctcgtga tcggcgacct
     1321 cgtgctcaac gtcgaccagt gggacggcta ccccgccgcc cgccagcggc tgctccagtt
     1381 catcgccgac aacaacatct ccgacgtcgt cgtcctcacc ggcgacatcc actcggccgc
     1441 tggtggcaac ctcgctcccg cgtccgacga cggcagccgc tcgcccgtcg ccgtcgagtt
     1501 cgtgggcacc tcgatcacgt cgggaagcct cgtcgacatc gttcccggcg gcgccgctct
     1561 cgtgaccgcc gacaagttcc ccggggtggc gtacatcaac gtccgcgatc acggctacgg
     1621 ccgttgcacc gtcacccccg gtgagtggac gaccgagttc gtcgtggtcg agtcggtgaa
     1681 gaccgccgac gctgcggcac gggtcgacgc cacctgcgtc accaagcagg gcgaaccggt
     1741 cgtcacgaag ctgtgaattc gcggggccgc gccgtgatgg gcgctccccc gccaataggg
     1801 tttccgcatg agcaacgcca ccaaggccat catcggagct gtcgtcgccg tcgccgtcct
     1861 cgcgttgggc ggcacctggc tgtacatcaa tgtgatcaag gacgatgcgc ccgagaagct
     1921 gtccctcgac gacacggcgt cgggttccga cgccacgacg acgaccgccg cgtccggcga
     1981 cgctccgaca acgacgagcg gcgaggcacc ggcggccggt gacggcgtcg acggcgagtg
     2041 gacggccacc gacgcgtcgg tcgtcggcta tcgcgccaag gaggtgctgt tcggccagga
     2101 caccgagggc gtcggccgga ccaacgcggt cagcggcacc ctgacgatcg ccggcacctc
     2161 cgtcgaatcg gcctccttcg aagtcgacat gaccagcctc aagagcgacg agtccaaccg
     2221 tgacaaccag ttcaacggcc gcatcatgga gacctccacc tacccgaccg cgacgttcga
     2281 gctcacctca ccggtcgacc tcggctccgt ccccgaggac ggcgtcgaga tcacggccac
     2341 ggcgaccggc cagctgacgc tgaaggacca gaccaaggac gtcgacctcg acatcactgc
     2401 caagcgggag ggctcgacca tcagcgtgct cacgagctac tcggtgaagt tcgccgactg
     2461 gaacatcgac gaccccagct tcgggccggc ccaggtggag gacaacgccc tcctcgagat
     2521 caagctcgtg ttcgaacgag cctgacgatc gcttcggttg tcgggctcgg agctacatcc
     2581 agggacgatc gcggcgggag atcatcggct tggtccacca gcggcccgac cagcgccagc
     2641 gcggcgaatc gtcgcggctc ggctcggccg caccccttgg ccacgcaccc tcgatcgcag
     2701 cgacgatcgc cactgcttcc tcgtcggtgg ggaccgggtt gatctcggtg ccggccatca
     2761 gagcggcatg ttgccgtgct tgcggggcgg caacgattcc ttcttggagc ggagcatcgt
     2821 gagaccggcg atgagcttga gccgggtttc ggccgggtcg atcacgtcgt cgacgtaacc
     2881 acgatcggcg gcttcccacg ggttgcagaa ctgctcggtg taggcgtcga tgagctcggc
     2941 ccggcgggcg acggggtcgt cggcctcggc cagctcacgc cggttcacga tctcgacggc
     3001 gccggacgca cccatgaccg ccagctccgc ggacggccag gcgaaagcga gatccgagcc
     3061 gatggacttg gagtccatca ccacgtacgc cccgccgtag gccttgcgcg tgatcaccga
     3121 gatacgcggc acggtggcct cgcagtaggc gtagagcagc ttcgcgccgt gacggatgat
     3181 gccgttgtgc tcctggtcga cgccgggcag gaagcccggt acatcgacga acgtgacgag
     3241 cgggatgttg aaggcgtcgc aggtgcggac gaacttcgcc gccttggacg acgactcgat
     3301 gtcgagcaca ccggccatcg ccatcggctg gttgccgacg atgccgaccg actggccgcc
     3361 gagacgggcg aacccgcaca cgatgttggg tgcccacgcc gaggcgtact cgaagaactc
     3421 gccatcgtcg gccaccgtcg agatgacctt gcgcatgtcg taggcctggt tggccgacgc
     3481 cgggatcagc tgctcgagct cgggacagag gcgatcggac ggatcgtcgg tctcgaggcg
     3541 gggcgcctcc tcgaggttgt tcgccggcag gaagctgagc aggtaccgca cgtcgtcgag
     3601 ggcggccttg tcgtcgccgg acacgaactg ggccacaccc gacttgctcg tgtggacccg
     3661 ggcaccgccg aggtcctgct ggctgacgtc ctcacccgtg acctgcttca cgacgtcggg
     3721 acccgtgatg aacatgtagc tcgactgatc gaccatgaag atgaagtcgg tcatgatcgg
     3781 gctgtacacc gagccgccgg cgcacgggcc catgatcacc gagatctggg gggtgacgcc
     3841 cgaggcgagc acgttgcggt agaagatgcc gccgtagctg gcgagcgaga ccacgccctc
     3901 ctggatgcgg gccccggcgc cgtcgttgag accaatgacc ggggcgccca ccttgagggc
     3961 catgtccatg accttgtgga tcttctcggc gtagacctcg ccgagggccc cgccgaacac
     4021 cgtgaagtcc tgcgagaaca cgaagacctg gcgaccgtcg acggtgcccc acccggtgat
     4081 gacaccgtcg gtgtagggac gctccgtggc agcggcgttg cgggaccggg ccagaccgtc
     4141 gagctcgtgg aacgacccct cgtcgaggaa gtagtcgatg cgctcgcggg cgagcatctt
     4201 gccgcgcgag tgctggcgtt cgatcgagcg ctcggaaccg ggggtgagcg cttcctgctt
     4261 gcggcggcgc aggtcctcga ctcgttcaga caacgggtga tccataggcc acagaggata
     4321 aagccaaccg caaccgatgg ccgaatcgct agcgtgaccc gatgacttcc ccgattcccg
     4381 ccgcagcgac cgccgattcg cgtctcccca tctgcgtgta cctggcctcg cgccacggcg
     4441 cgaacccgtc ctacaccgag gagacccgcc gactcggcgc ggcgctcgcc gcagcggggt
     4501 gcgacctcgt gtacggcggc ggcagcgtcg ggctcatggg cgaggtcgcg gacgccgtgc
     4561 tcgccggtgg cggtgcggtc accggagtga tcaccgagca cctctacaac ctcgaggtcg
     4621 ggcaccagca ggtcaccgag ctgatcgtcg tgcccgacat gcccacccgc aagagggcga
     4681 tgttcgaacg cgccgagggc ttcgtcatcg ctcccggggg agtcgggacg atggaggagc
     4741 tgttcgaggt gtggtgttgg gcggcgctcg gcctgcaccc caagacgctc ggcatgctga
     4801 actgcgatgg ttactacgac cacctactcg cgttcatgca gcgctccgtc gacgagggcc
     4861 tgatgtccca gcacaccctc gacctgctcg tggtcgacga cgacccnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnncccg tcggcgacat cctgttcgaa ccgaagtggg
     5041 acgggttccg ctgcatcgtg ttccgcgacg gggacgaggt catcctcgga agtcgcaacg
     5101 acaagccgct gacccggtac ttccccgaga tgctcgaccc gatccgctcg atgctgcccg
     5161 accgctgcgt cgtcgatggt gagctcgtcg ttgccaccga caagggcctc gacttcgacg
     5221 tcctcggcca gcgcatccac cccgccgagt cacgcatcaa ccggttggcc gccgagacgc
     5281 cggcatcgtt cgtcgcgttc gacctgatcg cgctgggcga cgacgacctc cgcgagacac
     5341 cgttcggcga gcgccgccgg ttactcgagg gcgtcttcgc cgacgtggtc ccgcccctcc
     5401 atctcacccc gatcaccccc gaccgcgacc tggccgccga ctggttccag cgcttcgagg
     5461 gggccggctt cgacggggtg atggccaaac cggtcgccga tccctacctg tcgaacaagc
     5521 ggtcgatcct gaagatcaag caccagcgca ccgccgacat ggtcgtcgcc gggtaccgcg
     5581 tccacaagga cggcaacggc gtcggctccc tcctgctcgg cctccacaac gacgacggcc
     5641 agctgcagca catcggggta gcgtcgagct tcagcgccgc ccgccgggtc gagctgctcg
     5701 acgaactggc gccgtacgcc gatcgcgctc tcgacgacca cccgtgggcg gcctggggca
     5761 acgcccgggc gcacgaggag agtcgcatgc ccggcgcgcc gagtcggtgg tccggcggca
     5821 aggaccagtc gtgggtgccc ctccggtgcg aactcgtggc cgaggtcggc taccagggcc
     5881 tgacgtcggg ccgcctccga cacccggcga agttccttcg ttggcgcccc gacaaggatg
     5941 ccgaccagtg ccggtacgac cagctcgacc aagcggtccc gatcgagctc gagcagctct
     6001 tcggcgacga ccgtttctcg aactgacgac ccgccgcccg ccctgaacgg cgttcagttc
     6061 cacaggggcg cggctaccat gctccggggt tcgaatcggg ggagacgacc atgaacacgg
     6121 gacggagaac gctcgcggcg ttgatcgcgg cgctcagcat caccgcggtg gcggcctgca
     6181 gcgaggccga cacacccgag gcggcctcga cgaccgcggt ccccacgaca cgaggcagcg
     6241 gtgcgcccga acccaaggcc ggcgacgggt ccgtcgtctc caagttcgct ggatccccct
     6301 ggctcctggg cgatgttccc gagcgggcca ccccggcgga ctcctcgaag ccgccgatca
     6361 aggtcggctt catgaacatc gactccggtc ccatcgcggc gacgcccgag ctgcaccagg
     6421 caactgacgc agcgatcgcg ttcatcaatg ccgagctggg cggcgtcggc ggccgaccga
     6481 tcgagatcgt gttctgcgaa accgatctcg gcgtcgacaa ggcgcaggcg tgcgcccgtc
     6541 tgatggtcga acaacaggtc gtcgcagtgc tcggcggcct caacctgtcg gccggtgcgg
     6601 ccacgaagat cctcgaggag aacgacatcg cctgggtcgg cggcatcccg ctggacccag
     6661 ccgagatggc gagccccgtc gcgttccagt tcagcggcgg tgcaccgggc gcgttcgtcg
     6721 ccttcgccga ccacgctgcc cacgagctcg gagcgaagcg ggtcgccgtc ctctacgcca
     6781 acactccaca gatctcggtc gccgctgtcg actacggcat cgccatgctg aaggccctgg
     6841 acgtcgacgt gaccgagatc gcattcgatc tcacgaccca ggactacgcc tcgatcgtgc
     6901 aacgcgccgc cgaggcgtcg cccgacgcga tgctggtggg agcggccgat ttcgcgtgcc
     6961 ccaaggtgat gcaggccatc gccgatctcg cactcgacac gacgatctac atggtcggat
     7021 cctgcgcaga cgccaaatgg atcgaacagg tcggcgccga gaagatcgcc ggcacgatct
     7081 tcaacatcga gtcccgcgtc gaacgcaccg gcccctacga cgccgacatc gagatctaca
     7141 cgtcggtcat cgaccagtac ggcaagggcg tcaacgccaa gggcgctgca accgtggcgt
     7201 tccggggcgc catgaacctg tatgcggtgc tcaccgatct gggcgacgac ccgacctcgg
     7261 cagaggtcat cgacgcgttc cgcggcgcga aggaacggaa gagcttcgac ggctacccct
     7321 acacctgcga cggcaagcag atccccgagc tcccgtccct ctgcgcctcc caggaggtgc
     7381 tcgtgtcgat cgccgccgac ggacagacgt tcagcgagga atccgacggc tggatcgacg
     7441 tcccgaaact cgtcgccgag tacctgcggt gatgtgaccc aacacctggc gtacctgatc
     7501 ctcgggctcg gcgccggcgc cgcgctctcc ctcctcgcct gtggggtggt cctgaccgag
     7561 cgcgcctcgg gcatggtgaa cttcgcgcac gcggcgatgg gcatgtacct cgcccacgcg
     7621 ttctacgagt tccgcgagac cggcgacctc gtgttgccga tcgtcgggct tccggcgcgg
     7681 gtgcacctgc tcccccgccc gaccgtcgcc accgcagtgg tcgtgatggt cgtgtacgcc
     7741 gcgttcctcg gcgcggccgt ctacctgctc gtcttccgtc gactggcgaa ggcaccggcg
     7801 ttggcccgag ttgtcgcgtc gatcggcctg ttcctgtacc tctgggcgat ggtcgggctg
     7861 cggttcccga accccccggc catccgccgg atcctgccca gccgagcggt ccagatcctc
     7921 ggccaccccg tgttccagga ccggttgtgg tcggccgtcg tcgcggtcgt ggtcacggca
     7981 ctcctctggg ccaccttccg gttcgctcga ttcggactgg cgaccatcgc cgcgtccgag
     8041 tcacgcaagg gtgccgagct caccggaatc aacagcagcg ccgtggcgct cgtgaactgg
     8101 atgctcgcca ccgtcctggc cggcgtcgcc atcgtgctca tcgcgcccgt caaccaactg
     8161 gacccgctca acctgtcgct cctcatcgtc ccgacgctcg ccgcctgcct gctcggcggg
     8221 agccgttcct tcgtgaccgt ctccgccgcg gcgttcgcga tcggcatggc gcagtccgag
     8281 atcgtgaacc tccaggtcgg ctggcactcg ctctccggcc tgggcctgca gcaaggcctg
     8341 ccgttcctcc tgatcctcgc caccctcgtc gtgcgcggtc ggagcctgcc gagccgcggc
     8401 gaacccgagg cggcccgtct cccggcatcg ccggagccgc gcctggtggc gccgaccgcg
     8461 ctcgtcatcg gggcagcagc gatcctggtc atgctcgtgg ccggcccgag ctggcgttcc
     8521 gcggtgatca cgtccgccat cgccgtgacc atcagcctct cggtggtcgt gctcaccggg
     8581 tacgtcggcc agatctcgct cgcacccttc gccttcgccg gcatcgctgc cttcggcctg
     8641 gcccgactga gcgactgggg gatcccgttt ccgttggccc ctctgttggc gatcgccggc
     8701 acgggcctca tcgggatcgc ggtcggtgtc cccgccacga aggtccgggg catgaacctc
     8761 gccatcgcca cgctcggagc cgctgtcgcc atcgagcaac tcctgttcaa gtggcggccg
     8821 ttcgtcgacg ccggcgtgaa cggcgccacc gagccgtcgc tgttcggggt caacctcggc
     8881 atctcggccc gcggctcgga cttcccgcgc tccgcgttcg gcatcttcac cgtggtcgtc
     8941 gcgaccgcgg ccgccgtcgg aatcgccaac ctccggcgag gtcgatccgg cgtcgcttgg
     9001 ctcgccgtcc ggtccaacga acgggccgcg gccgccgccg ggatcgacgt ggtgggcgcc
     9061 aagctgtccg cgttcgcgtt gtcgtccctg ctcgccgggc tcggcggtgt gctgctgacc
     9121 taccagcggt tgacgttgac caccgacagc ttcgccgtgt tcctctcgct ggcgacgctc
     9181 gcactcacct acctggccgg catcgcgtcg gtgagcggcg ccgtgctcgc cggggtgctc
     9241 acgccgctcg gcgtgttcgc ggtgatcagc ggacaggatc cggtgcgggt gagcccgtac
     9301 tcgttcgtcg tcaacggagc gctgctcgtc ggggtcgcgc tcgtctaccc cgacgggctc
     9361 actggcgcag cgacacgact ggctcgtggg gtggcccgcc gatgggggtc aacgggccgc
     9421 ggcgagacga gcgagcttcg cggcaacgtc gccggcgtcg agtagcgcgc acatctcggc
     9481 gaaccccgcg tcgggacccg tccaacgcca gtccgccggg gttcccacct cgacgtcgaa
     9541 ctcgagcgtg gcgatgcgcc ggaacaagag cgcgtcctcg aagtgttccg ccagggtcgc
     9601 gcacaacttc gcaccgccgc ggacggtgat gtcccactgt ccgggattga gcgggatgtc
     9661 ctcgatgtgg cggtaccggc tgagcaccgc cgcggcggac ttcgcacccc accccgccag
     9721 cccggggaac ccgtcggcgg tgtcgccgac gagccccaac cagtccggga tcgactcggg
     9781 ctcgacgccg aacttctccc gcacaccggc ctcgtcgcgg aacaccccgg cgcgccggtc
     9841 gaactgcacg atgtcgggat ccgacacgca ctgcgcgagg tccttgtcgg gggtgcagat
     9901 caccgcgcgt tcgatcgacg gatcggcggc cgccacccgg gccgcggcgc ccaacgcgtc
     9961 gtcggcctcc acctcgacca tggcccacac cgtgaatccc gccgaccgaa gcgcatcctc
    10021 gagcaggggg aactgcgcct tcaggacggg gtcgatcccg tcgccgttct tgtagccggc
    10081 gtagaggtcg ttgcggaagg actcgatgac gtgatcggtc gcgaccccga tgtgggtcgc
    10141 gccgtcgtcg agcatgccgg cgagcgaccg gagtaccccg cggacggcgc cgacctccat
    10201 tcccgacccg ttgagatggc caccgccggg cgaatagaag tagcggaaga gctcgtaggt
    10261 gccgtcgacc agatggacct gcgtgcccga gggcgacgcc gcgtgcgatg acggggacgg
    10321 agaagcgtcg ggagaggggt cagtcacggc tccagcctgt cacagacccc ttcgggacgg
    10381 cctgcctcag gcgggcgatc cgacgggctg acgcggctcg gcgtcgagat cgtaaccgag
    10441 caccttcgtg acgatccgac cgagggtgag gaactcctcg cgatcgacgc gatcgaccag
    10501 gcgcgcccga acgcccgcga cgtggtgcgg agcgacctcg acgagcagct gccgaccggc
    10561 aggggtgagg cgggcgatga agccgcggcg atcggtctcg cagttctccc ggacgacgag
    10621 gccacgctct tcgagacggt ccacccggta ggtgagccga ctccgggaca cgagcacccg
    10681 ggcggcgagc tcgctcatgc gcagaccgct ctcctccgcc tcggacagat gcacgagcac
    10741 ttcgtagtcg gcccccgtca agccgaacgc tgcgacatct gtgtccatgc cgtcgatgat
    10801 tcgcccgcag aactcgacaa cggctcgcca cgccagctgt tcgtcggcct cgagccacct
    10861 cactggttcc atctggcgac cgtaccctcg gtcgttccaa accgaacaga attgggtaga
    10921 tccgctcaac ccgtggcggg tcaaccttcc tggacgagtt cgatgagggt gccgaagctc
    10981 cccttggggt ggatgaaggc caccgtcgtg ccgcgactac ccgggcgcgg ggcggtgtcg
    11041 atcggcgtgg caccggccgc gatcatggac gcgagggcct cggcgcagtc gtcgacgcgg
    11101 tagccgatgt ggtgcaggcc ttcgccacgc ttctcgatcg acttggcgat gggcgagtcg
    11161 ggacgcgtgg ccgccgtcag ctggatgtag gagtcggcga ccttcaccag ggcctcctcg
    11221 acaccgtcgg actcgacgat ctcacggtgg tggacctcgg cgccgaacgc ctcggcgtag
    11281 taggcgatcg cggcatcgag atcacggacg gcgatggcga catggtcgat gttggtgagc
    11341 agcatccgac gatcattcca cgaggaggtc ggcgcgctcc atccgccgtt ccccggggca
    11401 ggccggcccc ccgtcgctac gacgccacaa tgccgtggac gtcgaggaac gaccgcagca
    11461 ccgccagccc ggtgtagacg tggccgacga ggcccgcggt gatcgcgccg tggacgttga
    11521 cgtcgaggtc gtcgacgaac gccgtggtgg tggggtcgac gtcgagcgcc gcgaccacgt
    11581 tgaggtacga ctccgggagg ggcttgggaa tgcccgtctc ggccgagttg aacacatggt
    11641 cgaacgcctc gtgcagaccg gcctccgcca actcggtccg ggtccggagc gagccgttgg
    11701 tgagcaacgc gaccggcacg accgcctgca ccgaccgcac gagcgcgacg gcctcggcgt
    11761 cgacctcggc tcgcatcgcg acgaactcca ccgccgccgc ctcggatccg atcagtgcgc
    11821 tgagcgccgc cgcgaacccg tcgtggtcga ggcgaccgca catgaactcg tggcctgcgc
    11881 tactcccgaa cgccgtcggc accagcgacc ccaccggaat cccgtggcgc tcctcgatgt
    11941 cgcgctcgtg cacccggtcg tagtggcgaa tcactccgtc gagatcgaag acgacggcgt
    12001 cgaggcgacg gggcggtcgg tccatcggtc gaacataccc gcaccccctg acgacggccc
    12061 cctggttctc gtcggtccac gacaccgcgg gtacgatcac cctcagcgcg tacccagggg
    12121 gacatcgatc aatgagtgac gtcaccccac cgtcgggcga cctaccgcca cccccgccgg
    12181 gcccgcccgg tccggggttc tggcaacaat cgtccacctc gctgcacgag cccgcccccg
    12241 aacggccgcc gacctacgtc accccacccg gtctccaaca gcccctcggg gccgcgccgg
    12301 gtccgtccgt acccgaagtc gccggattct ggcgacgggc ctgggcccga ggcatcacgt
    12361 tcctgttctg gctcctggtg ctcgacgtcg gtacgtacct catcgcactg ctgttcctct
    12421 tcgtttactc cgccaccccg aacgactcga tcctgctcgc cggactcgcg gtgtcgtggg
    12481 cgtacgggct cctcgtgacg tacctcatct ggtcccggtc gctggccacc cggcgcggca
    12541 cgctcgggat gcgccagatg gggctctcca tccgcagcgc gtcgcgccag ccggccatct
    12601 cgaaaggcca ggcctttcgt cgcagcatca tcgccctgct gccgttcgtc ttcgcgagcg
    12661 gagcggggtg gctcctgctc tacacacccg acgacgacct catcgtgctg tcgctcatcg
    12721 tgcaggccgc cctggtgggc ctcgtcaccc tcggtggcct gtggatgctg gtcagtcctc
    12781 gccgacagac cgtgtgggac atcgtcggcg acaccgtggt cgtcgtcgac cgcgagccca
    12841 gctggctcgc cgtcagtgcg cttgtcgccg ggctgctggt cccgacgggc tcgatcgtct
    12901 cgctggtcgt ggtcggcgcc gcccggctcc agtcggagta ccgctcgctg tcgggctacg
    12961 gcagcgccgg tcggtacttc ctctacaaca tccccaccct ggtgctctcg gtcatcgcca
    13021 tcgcgctcgg acacgtcgcg atccgcgcga ccaggtggga agcccaacgg ctcgccggtc
    13081 ggggcctcgc ccggaccgcg ttgttcctcg gctacctgtt ccccgtcgcg ttcgtcgcaa
    13141 ccctcgcctt cggcgcgatc taccgccagg tcgaggatcg ccaggtgcgg tcctgttcgg
    13201 acacgcgcga cgacatcgcc gtcgcggcgg agtcgtaccg cctgctcaac ggcgtctacc
    13261 ccgacagcat cacgccgatg gccgacggca tctacctcga cgatcccgag ctcgccgaac
    13321 gttggacgct ctccgatgag tcggcctcgg gcgttcccga gtaccgactc accggcaagg
    13381 gcgactgcgc gaaggcctga cgatcaggcc agctcgccgc gggcgtgccg tgcgaagagc
    13441 gtgatcttgt cctcaccgat ctcggcgcgg cgctcggggt tggtgatgcc ctgaccggcc
    13501 tccggagcga ggcagagcac accgagcttg ccctccgcct ggttcttgtg gaccttgagg
    13561 gcggcgtcgc cgacctggtc gagcgtgtac acctcgctca tcaccggctg gatgcgaccc
    13621 tggccgatga gctggttcat gttccacgcc tcctggtagt tggcgaagtg gctcgacacg
    13681 atgcgcttga gcttcatcca caggtgacgg ttgtcgaact cgagcatgta gcccgacgtc
    13741 gccgcacagg tgacgacggt gccgccgcgc ttggcgatga acaccgaggc gcccatggtc
    13801 gagcggcccg ggtgctcgaa gacgatgtcg gggtcgtcgc cgatgagcga gcggaccttc
    13861 ttgcccagac ggcgccattc gccttcatcc tgggtgtgct cgtcggacca gaactggtag
    13921 ttctcggcgc ggcggtcgat cgtgttctcg caccccatcg cctcgagcag cttgactcga
    13981 tgctcactcg acacgacgcc gacgggcgtg ccgccgccgt tgaggatcag ctggatggcg
    14041 taggcgccga tgccgccggt cgcgccccag acgaagacgt tgtcgccctg cttcattcgg
    14101 gcagcgtggc tcccgacgag catgcggtag gacgtggagg cgcagagcgc gttgaccgcc
    14161 gcctcctccc agctcaggtg cgccggcttc ggcatgagct ggttggcctt cacgatggac
    14221 aggtcggcga ggccgccgta gttggtctcg aacccccaga tgcgctggtt gctggccaac
    14281 atggagtcgt cgtgggccga cggatcctgg tcgtcgaggt agttgcagtg gaccgtgacg
    14341 tggtcgcccg gcttccagtt gcggacggcc gagccgaccc ggagcaccac ggccgaggcg
    14401 tcggaaccga caacgtggaa gtcctgcttg tggcgggcgc cccactcgga ctccttgccg
    14461 agacgatcga ggaaattgaa ggtcgacagc ggctcgaaga tcgacgtcca cacggtgttg
    14521 aagttgatcg aggaagccat caccgcgatg tagacctcgt cgggggcgat ctcaggcgtg
    14581 gcgacctcgc cgacgtgcag cgacttgcgg ggatccttgt cctccgacgc gaacccggcc
    14641 cacatgtcct gctcctcacg gagcacgtag gcggcgcgga acgactccgg cagagggagg
    14701 ttggcgatct cgtcgccgct ggcgtcggac tggatcgctt cgaggatgtg ctgcatcgac
    14761 atgggtcaag acggtagcga cttcaatgga gcgatggcga gaagacgcgg cacgggtgcg
    14821 accgtcgcca tcgttgccct ggcgtcgatc gggctcgtcg cgggatgcgg agacgatcgc
    14881 cccacctcca cccggtcccc gacgaccgtc gtcgatcggg ccccgcgaac ccgccccacg
    14941 acgacgctcc ccgacctcac cgcctccgac ggcctcgccg cgtcgatccg acagttccgc
    15001 gaggacgagg cggcgggcgt gatccagatc gagatggtcc gcgaacccgg gggcgaggtg
    15061 ctcgtcgcag tgacgtcggt ccggctcgac tggcccggct tcgaagcggt tccacccacc
    15121 gtccgccccg tgcaggtcgc cccgggccag cgggtc
//
LOCUS       DAOSOA010000032        15106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3966_length_15106_cov_7.172879, whole genome shotgun sequence.
ACCESSION   DAOSOA010000032 DAOSOA010000000
VERSION     DAOSOA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15106
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3966_length_15106_cov_7.172879"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKY13_03750"
     CDS             <1..870
                     /locus_tag="PKY13_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HOT79072.1"
                     /translation="ADTQQTDTYYIVAHFHYVLFGGALLGLFAGFYFWWPKIFGYFLN
                     ERWGKVHFWVTLVGFNLTFGPMHVLGLQGMSRRYFTYSADQGFSFWNMVATVGSFIIA
                     VGLLIFMANVWKSHKAFKKEPYEVPADPWDARSLEWMIPSPVPEYNFDEIPEVHELDE
                     FWHRKYGHDEDGRLVRIAKTEDIVQKPGATGIHLPSPSYWPIILSAGLPFIGYGVIFD
                     YWWAVLGGLITVAGFLGWILEPSTDPEDHHADHGHGDHDAHQLEAGEEQPALVGSTDA
                     PAAEASNEETSGD"
     gene            863..1540
                     /locus_tag="PKY13_03755"
     CDS             863..1540
                     /locus_tag="PKY13_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HOT79073.1"
                     /translation="MTDTLENTSHDIEDPNFDIESVGHTHLPTNTGVSNEKLGMWVFL
                     GSECLMFGGLISTYLLYKNRPGAIESIRGRDIVSSSLRPSDLFDIPFTSVTSFVLLAS
                     SLTMVLAVNAAARNDMQRMRIWLGSTAALGSLFIGGQVYEFTTFHREGLGFTTNVASS
                     AFFTLTGFHGVHVTIGILMLLVTIAMSIRGRIPHSRAEAVEIVGLYWHFVDVVWILIF
                     TIVYLIK"
     gene            1551..>1969
                     /locus_tag="PKY13_03760"
     CDS             1551..>1969
                     /locus_tag="PKY13_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOT79074.1"
                     /translation="MAAITATDAHGAHDEHPSDVVYWKVFGVLLALTALEVSTYWWPE
                     DWHKVTAVLLIIMMVIKFATVALYFMHLKSDAAILKRVFIAGMVLAVGVYVAVLGAMV
                     FFKDSGTAIQGGTGFNDPPREKPLPPPATDPPPIIRDT"
     assembly_gap    1970..2069
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2271..2993)
                     /locus_tag="PKY13_03765"
     CDS             complement(2271..2993)
                     /locus_tag="PKY13_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT79075.1"
                     /translation="MRATLAQYRIELALSLRNGEQLLVNLFIPLGLLIFFSKVKVLNS
                     DDVDPVEFLAPAVLGLAVMSSALVSLGISTGFERFYGVLKRLGATPLGRPRWVLAKFA
                     AVLTIEFGQWAILIPTGLLLGWSPAGGWPTAVLGAVLGTAAFGGLALFMAGTLGGMTN
                     LAACNGIYLALMVTGGMVVPFDKMPEALQAVAKVFPAAPLADVMIGSLQQGEHVHGAS
                     WLVLTAWAVVMPLLAAWRFSWD"
     gene            complement(2990..3970)
                     /locus_tag="PKY13_03770"
     CDS             complement(2990..3970)
                     /locus_tag="PKY13_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT79076.1"
                     /translation="MPIVEVDRLQVRYGDVVAVDDISFGAHEGDITVVLGPNGAGKTS
                     TIECLEGYRRPTSGSVRVCGLDPRTDRTELNRRVGVMLQSGGTYTAIRPIEAVRLFAS
                     YYDTPRDPERLLEFVGLGHRATTPFRSLSGGEQQRLSLALAIIGRPEVVFLDEPTAGV
                     DVSGRQLIRDLIRSLATEGVTVMLTTHDLAEVEALADRIVIIDNGRVVADSTPDELLA
                     GGDAEEFAFRARPGIDLSALGAALQGPVAELEPGRYRVGLAPTPATVASLTTWLAGQD
                     ILLGDLQAGRQQLEEVFLKLTAEPDAAAESRRGGRGGARARRRGHSADAP"
     gene            complement(3980..4648)
                     /locus_tag="PKY13_03775"
     CDS             complement(3980..4648)
                     /locus_tag="PKY13_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT79077.1"
                     /translation="MPDVPRPIIFDLDGTVWDSAPGIVSCMEATLHELGLPDPGRAAL
                     LGLLGPPIMAMLAGIGVPEDRLDDARVIYRRHYRDHGEFECEVYPGVPDLLDRLRASG
                     HALATGTSKGVEVAERMLEHFDLRRRFDVVAAASMTQPGHNKIDVIGEALGDLEAIGA
                     PATSAVMVGDRNFDVDGGRHFGLLTIGVSWGYAPDDELTDAGADHVVTTIDDLAGLLL
                     GHTK"
     gene            4794..5411
                     /locus_tag="PKY13_03780"
     CDS             4794..5411
                     /locus_tag="PKY13_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79078.1"
                     /translation="MDESIRDITPTTTIAPAPTQPGATVPAVTVAGSEFVARVAAVDT
                     SELVLGAAFNRQFLTLQFLAVGLDATESKCAADSITAASGDEFASRPVSQVMSGAGIT
                     PDVLLPCVSTDRMVALSKAGAGADLKRIPPDVLRSTLTELASAGYETVGLTPVESTCL
                     AERVVGTYTDADLAEVMTTLSLPADRAVTALPTCVTGERANELGA"
     gene            5439..6719
                     /gene="serS"
                     /locus_tag="PKY13_03785"
     CDS             5439..6719
                     /gene="serS"
                     /locus_tag="PKY13_03785"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT79079.1"
                     /translation="MLDIRLFRNELDQVRAGLARRGESMDSIDDVIRLDTEVREVNTS
                     RDDLRSRIRAISGEVGQLMRDGLKEEAGELQAESRSLGEREKELDAHSATVAAELRDL
                     LLRLPNLPADDAPDGLGEIDNVVTRIEHFDPDSYQDHQRVAHWDTGEALGILDVERGV
                     KMSGSMFVMYRGSGATLVRALCQLALDRNADLYEEVRPPTLVRTDTMVSTGHLPKFAE
                     DAYHLERDDLYAIPTAEVPLTSLARDEILDEAVLPMRLMAHTSCYRREAGSAGKDTRG
                     LLRVHEFDKVELLAYATADQAAGIHVEILARAEALLTDLGLAYRVLDLCAGDLGNSSR
                     RTFDLEVYAPGVDRWLEVSSVSWFGDYQARRANIRYRSAGGKGTEVCHTLNGSALAVP
                     RVWAGIVETHRQPDGRVLIPEVLRPYMRGLTHLG"
     gene            6721..7392
                     /gene="pdxH"
                     /locus_tag="PKY13_03790"
     CDS             6721..7392
                     /gene="pdxH"
                     /locus_tag="PKY13_03790"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOT79080.1"
                     /translation="MSGVDPEFTQPSFEGERNNYDVGGLDEAAAAADPFVQLRRWLDE
                     AAEAQVREPTAMTVSSLGIDERVHSRTVLLRQLTSSGLVFFTNRDSHKGEQLAAHALC
                     AAQLLWLDLHRQVRVEGIARLLDDRVADDYFAGRPRGSQIGAWASPQSQVLRSREELE
                     ARVEAAERQFADVDIVPRPPNWGGYCIEPDYFEFWQGRPSRLHDRLRYVVGPDGHWKR
                     DRLAP"
     gene            complement(7403..8116)
                     /locus_tag="PKY13_03795"
     CDS             complement(7403..8116)
                     /locus_tag="PKY13_03795"
                     /inference="COORDINATES: protein motif:HMM:NF037471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79081.1"
                     /translation="MSGFDDTLERHFPGAVPSGVFLNQVWTSTEELGFKVGNTLPLIS
                     ICRDELTTDFFARIQAIWGPAFTLAGLGGVPALGRTGWGAAFSHIPDDAGRGLVLVLG
                     FPHIGIEDDGSVGVTLRPGQSVATNTCGALAAIHQRSAEGTLPTEIDLDDYEATRLAL
                     RLVDPADPPVSLVDLTIATLEAVEVDLWKALDEARIWEHHDVAVFCGVQIHGHGGADW
                     IWPRDAWVCGSDGERRPVL"
     gene            complement(8132..9010)
                     /locus_tag="PKY13_03800"
     CDS             complement(8132..9010)
                     /locus_tag="PKY13_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOT79082.1"
                     /translation="MSTATLLLAFSAGMVAAVNPCGFALLPAYLAYYLGLESSGDADA
                     PPRGGELGRSLAVSGAMTAGVVLVFGLIGAVWSGVSEVVGQRLPWVIVVLGIALVFLG
                     IAMVAGYVPMIRVPKLATTSGSQSAWSAFVFGVSYGVASLSCTIGPFIGTVTTTFRRH
                     DFLTGLGTLVIYGLGMGAIITVLTLAVSFAHHGVVTRLRRLLPHMGRISGVLLIVSGL
                     VATYYGWYEARILTGGSADNAFAETIAGWRDGLVRWITDVGEERVGIAVAIILVAATT
                     ITVVLRRSRVGTGRGD"
     gene            complement(9030..9647)
                     /locus_tag="PKY13_03805"
     CDS             complement(9030..9647)
                     /locus_tag="PKY13_03805"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT79083.1"
                     /translation="MSTEPENAPPRARSPHKFIGAILLAAALALFVSWLVVLRPALDD
                     NSAIGVDDLVNSDARAPSDGGPTAEVGKIAPDVTLKGFDGADVALSSLRGKPTVINFW
                     ASSCVPCIKEMPLIERAYAELGSDVNFVGVDVFESPDLGRDMITRTGVTYPQTVDPTN
                     EVLTTFGGVQLPHTVVLTANGTVSALHNEAITDDEVLRDLIDAAS"
     gene            complement(9834..10181)
                     /locus_tag="PKY13_03810"
     CDS             complement(9834..10181)
                     /locus_tag="PKY13_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT79084.1"
                     /translation="MTAAQTIQLEPDTAWERDAACADGGTETARLFFSDEITDIADAK
                     RICSTCPVIVECLDTALARGEQWGVWGGQLFVSGKIVMTKRRRGRPPKHPRPEDQLPV
                     IPFPEHLRHLQPA"
     gene            complement(10456..10632)
                     /locus_tag="PKY13_03815"
     CDS             complement(10456..10632)
                     /locus_tag="PKY13_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79085.1"
                     /translation="MRNEILISNEVVLTATSLRASMQRCLASISTGKWFDAATKYEHA
                     AINPTPHSGPGGSS"
     gene            complement(10785..11912)
                     /locus_tag="PKY13_03820"
     CDS             complement(10785..11912)
                     /locus_tag="PKY13_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT79086.1"
                     /translation="MSSTRRTTESAPIERQEKLPASEDVTEVAPGIYRLQLPINLPGL
                     GHVNCFALEDSKGFTVVDPGFPDPGTAQALADRMAQIGAPVRRIHTVFVTHSHPDHFG
                     GAGRLRITNQADIVAHEHFTTLFNRDDDHNHDVELEGRRSNDGFDPARFADALLGDGP
                     IPELPTRRSPWGGTFPFPTSDEITWMRAWDDDGKRGFVDNEPNVRVADHEVIVLGGRE
                     WQAVHTPGHTGDHVCLFDPADGILLSGDHVLPTITPHISGMTHSRDSLADFFAALHKV
                     AALDGVQLVLPAHGHPFTDLVGRVDAIERHHVERLDDLARIGADIGDASVIEFSHHLF
                     KERSWGAMAESETFAHLEHLRLAGRATSRTVDGQLHYAIER"
     gene            complement(11909..12469)
                     /gene="orn"
                     /locus_tag="PKY13_03825"
     CDS             complement(11909..12469)
                     /gene="orn"
                     /locus_tag="PKY13_03825"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOT79087.1"
                     /translation="MLVWMDLEMTGLDSRTDVIVEIATLVTDDDLNIVAEGPDLVVHQ
                     PPEALANMDKVVVDMHTSSGLLDQIAASTITLEEAGAQTLAFIKQHVGTAGTVPLCGN
                     SIGMDRRFLAAYLPEIEEWLHYRSIDVSTLKELGRRWYPEALAAAPRKATSHRAMDDI
                     RESVAELAYWRSTLFRATDAPGTPSA"
     gene            12514..12957
                     /gene="dut"
                     /locus_tag="PKY13_03830"
     CDS             12514..12957
                     /gene="dut"
                     /locus_tag="PKY13_03830"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT79088.1"
                     /translation="MEIPIRRTDPTDQTLELPRVAKPGDAGVDLRSSIDVTIPPGGHR
                     ALVPTGIAVAIPAGYAGFVQPRSGLALKHGVTCLNTPGLIDSGYRGELKVLLINTDPT
                     DAFEITRGERIAQLVIQAVVEPVFVEVDELDASERGEGGFGHTGR"
     gene            complement(12969..14363)
                     /locus_tag="PKY13_03835"
     CDS             complement(12969..14363)
                     /locus_tag="PKY13_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79089.1"
                     /translation="MSARSRYRASRIVSGATRWALARDPDRHEAALDPRHVGWIASVW
                     ATIKLGIVGILTPIWAPAIMVRAATNNRRAQRLTAEFPQQMRAIAAGRTPAPPSNRVL
                     DIPADVRLAVFSDLHRCVPGRLDWPARQRTKHLYELILDYYADDDWSLCENGDIEDYW
                     IVGGSTYGAVYDLLRMLGAAMARYSHTALLTETYKTHLDQIVGNNPGIYRRIRDHFVH
                     GGRYFRTVGNHDNPNNRPMVADRLREHLGTFPLADYFALRTPTGQLLAVICHGHHTDG
                     WNAPGRDNLGKLSAWIANTLIDVPRLETPEGLPPAGAEEQMLAGEIPDRLIAVNPTFG
                     ANSSYDSLDEELLCEAVRTAGLDDLWLILGHTHFPVSAPVSRTGQRWDRYVNSGSGVT
                     DGVITAIEWDGTATADGGDPQVRLVGWAMAGKDTPPEAIVITPSGQRLARYVLEPDGD
                     RLLPSLHVPLSSDV"
     gene            14441..>15106
                     /locus_tag="PKY13_03840"
     CDS             14441..>15106
                     /locus_tag="PKY13_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT79090.1"
                     /translation="MTVNERAPEGERTLEAVRERLSMPLDEAMMTQRAVRRVLPDPVD
                     DDLVVHLIELALRAPTGSNGQNWEFVVVKDAATKAKFAKRYRQSWSIYGGIGRRVSKG
                     NPSMLKVLDAVQWQVDHFEEIPVLVVPCLKGNHAPFVPMPPFAPSSYYGSIYPSVQNL
                     LLAARSVGLGASLITLPLWSSTSARKILGLPISVMPACIVPLGWPPGRYGPTTRRPVG
                     DVTH"
BASE COUNT         2315 a         5031 c         5236 g         2424 t
ORIGIN      
        1 gccgacaccc agcagaccga cacctactac atcgtcgctc acttccacta cgtgctcttc
       61 ggtggtgcgc tcctcggtct gttcgccggc ttctacttct ggtggccgaa gatcttcggg
      121 tacttcctga acgagcggtg gggcaaggtc cacttctggg tcacgctcgt gggcttcaac
      181 ctcacgttcg gcccgatgca cgtcctcggc ctgcagggca tgagccgtcg gtacttcacc
      241 tactccgccg accagggctt cagcttctgg aacatggtgg caaccgtcgg ttcgttcatc
      301 atcgccgtcg gtctcctcat cttcatggcg aacgtctgga agagccacaa ggccttcaag
      361 aaggagccct acgaggtgcc cgccgatccg tgggatgccc gcagcctcga gtggatgatt
      421 ccgtcgccgg tcccggagta caacttcgat gagattcccg aggtccacga actcgacgag
      481 ttctggcacc ggaagtacgg ccacgacgag gacggccggc tggttcgcat cgccaagacc
      541 gaggacatcg tccagaagcc cggagccacg ggcatccacc tcccgtcgcc gtcctactgg
      601 ccgatcatcc tgtccgccgg tctgccgttc atcggttacg gcgtcatctt cgactactgg
      661 tgggcggtcc tgggtgggct gatcaccgtc gccggcttcc tgggctggat cctcgagccg
      721 tcgaccgacc ccgaggacca tcacgccgat cacggccacg gcgaccacga tgcccatcag
      781 ctcgaggcgg gtgaggagca gccggcgctg gtcggtagca ccgatgcgcc cgccgccgaa
      841 gcatccaacg aggagacgtc cggtgactga cacgctcgag aacacgagcc acgacatcga
      901 ggacccgaac ttcgacatcg agtcggtggg ccacacccac ctgcccacca acacgggcgt
      961 ctcgaacgag aagctcggca tgtgggtctt cctcggctcc gaatgcctca tgttcggtgg
     1021 cctgatctcc acctacctgc tctacaagaa ccgtcccggc gccatcgagt ccatccgggg
     1081 tcgcgacatc gtgtcgagtt ccttgcggcc gtcggatctg ttcgacatcc cgttcacctc
     1141 ggtgacgtcg ttcgtgctgt tggcctcgtc gctcacgatg gtgctggcgg tgaacgccgc
     1201 ggctcgcaac gacatgcagc gcatgcggat ctggctcggg tcgaccgcag ccctcgggtc
     1261 gctcttcatc ggcggtcagg tgtacgagtt cacgaccttc caccgcgagg gcctgggctt
     1321 caccacgaac gtcgccagct cggcgttctt cacgctcacc gggttccatg gtgtgcacgt
     1381 gaccatcggc atcttgatgc tgctggttac catcgccatg tcgatccgag gtcgaatccc
     1441 gcattcccgg gccgaggcgg tcgagatcgt cggcctgtac tggcacttcg tcgatgtggt
     1501 gtggatcctg atcttcacca tcgtctacct gatcaagtaa tcgaggacgc atggctgcca
     1561 tcactgcaac cgacgcccac ggcgctcacg acgagcatcc gtccgacgtc gtctattgga
     1621 aggtcttcgg tgtcctcctg gcgctgacgg cgctcgaggt gtccacctac tggtggcccg
     1681 aggactggca caaggtcact gcggtgctct tgatcatcat gatggtgatc aagttcgcca
     1741 ccgttgccct ctacttcatg cacctcaagt ccgacgcggc gatcctcaag cgggtcttca
     1801 tcgccggcat ggtgctggcc gtgggggtct atgtcgcagt cctcggagcg atggtgttct
     1861 tcaaggattc gggaacggcg atccagggag gtaccgggtt caacgacccg cctcgcgaga
     1921 agccgctgcc gccgcccgcg acggacccgc cgccgatcat ccgcgacacn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgagaagccg ctgccgccgc ccgcgacgga
     2101 cccgccgccg atcatccgcg acactggcca tcactgaggg cctcggccac cttcgctagg
     2161 tacggtcgct tcgctcccta ctttcgctcc ggtacctcgg ccgggtagtc cttacatcgg
     2221 cgtcggcttt cagagccggc gccgatgtcg cgtctgggcc caggccagta tcagtcccaa
     2281 ctgaagcgcc aggcggcgag gagcggcatg accaccgccc aggccgtcag gacgagccac
     2341 gaggcgccgt ggacgtgctc gccctgctgg agcgagccga tcatgacgtc ggcgagcggg
     2401 gcagcgggga acaccttggc aacggcctgg agcgcctcgg gcatcttgtc gaacgggacc
     2461 accatgccac cggtcaccat cagggcgagg tagatgccgt tgcacgcggc gaggttcgtc
     2521 atcccgccca gcgtgcccgc catgaagagc gcgagcccgc cgaaggccgc ggtacccagc
     2581 acggcgccga ggaccgccgt gggccagccg cccgccggcg accacccgag caggaggccc
     2641 gtcgggatga ggatggccca ttgcccgaac tcgatcgtca acactgcggc gaacttggcc
     2701 agcacccatc ggggacgacc gagcggggtc gcaccgagtc gcttcagcac gccgtagaag
     2761 cgctcgaacc cggtgctgat gccgaggctc acgagtgccg acgacatgac cgccaagccg
     2821 agcacggccg gggcgaggaa ctcgaccggg tcgacgtcgt cgctgttgag caccttcacc
     2881 ttcgagaaga agatgaggag gccgagcggg atgaacagat tgacgaggag ctgttccccg
     2941 ttgcgcaggc tgagcgcgag ctcgatccgg tactgagcga gcgtggccct catggagcgt
     3001 ccgccgaatg tccccgtcgg cgcgccctgg ctccgccgcg gccgccccgc cgggattcgg
     3061 ccgctgcatc gggttccgcc gtcagcttca ggaagacctc ttcgagttgc tggcgcccgg
     3121 cctggaggtc gccgaggagg atgtcctgcc cggcgagcca cgtggtgagc gaggcgacgg
     3181 tcgccggcgt cggggccagg cccacccggt agcggccggg ctcgagttcg gcgaccggcc
     3241 cctgcagcgc ggccccgagg gcgctgaggt cgatgccggg acgggcgcgg aaggcgaact
     3301 cctcggcgtc accgccggcg agcagctcgt cgggtgtgct gtcggccacg acgcgtccgt
     3361 tgtcgatgat cacgatccgg tccgcgagcg cctcgacctc ggccaggtcg tgggtggtca
     3421 gcatgacggt gaccccctcg gtggcgaggg agcggatgag gtcacggatg agctggcggc
     3481 ccgacacgtc cacccccgcc gtcggctcgt cgaggaagac cacctcgggg cggccgatga
     3541 tcgcgagcgc caggctcagc cgctgttgct cacctcccga caacgagcgg aacggcgtgg
     3601 tcgcccggtg ccccaggccc acgaactcga gcaggcgctc cggatcgcgt ggcgtgtcgt
     3661 agtagctggc gaacagccgc acagcctcga tggggcggat ggcggtgtac gtgccgccgc
     3721 tctgcagcat gacgcccacc cgacggttca gttccgtccg gtcggtgcga gggtcgagtc
     3781 cgcacacccg caccgacccc gacgtgggcc ggcggtagcc ctccaggcac tcgatggtcg
     3841 acgtcttgcc ggccccgttg gggccgagta ccaccgtgat gtcaccttcg tgggcgccga
     3901 agctgatgtc gtccacggcg acgacgtcgc cgtagcggac ctgcaggcga tcgacctcga
     3961 cgatgggcac cgtgtgacgt tacttcgtgt gccccagcag caatccagcc aggtcatcga
     4021 tggtggtcac gacgtggtcg gcccccgcgt cggtcagctc gtcgtcgggt gcgtaccccc
     4081 aggacacacc gatcgtgagc aggccgaagt gccgaccgcc atcgacgtcg aagttgcggt
     4141 cgccgaccat caccgcgctc gtggccggcg caccgatcgc ctcgaggtcg ccgagcgcct
     4201 cgccgatcac gtcgatcttg ttgtgccctg gctgggtcat cgacgccgct gccacgacgt
     4261 cgaagcgacg acgcaggtcg aaatgctcga gcatccgctc cgcgacctcc acgcccttgg
     4321 aggtcccggt cgccagcgcg tggcccgatg ccctcaggcg atcgagcagg tcgggcacgc
     4381 ccgggtacac ctcgcactcg aactcgccgt ggtcgcggta gtggcggcgg tagatcaccc
     4441 gggcatcgtc gaggcgatcc tcgggcacgc cgatcccggc gagcatggcc atgatcggcg
     4501 gacccagcag tcccaggagc gcagcgcgac ccgggtccgg cagaccgagt tcgtggaggg
     4561 tcgcctccat acaactgacg atgccgggcg cggagtccca gaccgtgccg tcgagatcga
     4621 agatgatcgg gcgtgggacg tcgggcatct cggcgaggct agtgagcggc cggaccccta
     4681 cgatgtgggg cgtgggaccg atggggggct ggacgacgtg gcggagacgc gccgcgatcg
     4741 ctgttggggc gagcgccgtg ctgctcggtg gcgcgagctg tacccagcga acgatggacg
     4801 agagcatccg cgacatcacg cccaccacga ccatcgcgcc ggcgccgacc cagccgggag
     4861 cgaccgtgcc ggccgtgacg gtggccggca gcgagttcgt ggcccgcgtc gcagccgtcg
     4921 acacctccga gctggtactg ggtgctgcgt tcaaccggca gttcctcacg ttgcagttcc
     4981 tggccgtcgg gctcgacgcg accgagtcga agtgcgccgc cgactccatc acggccgcat
     5041 cgggcgacga gttcgcctcc cgcccggtca gccaggtcat gtccggcgcg ggcattactc
     5101 ccgacgtgct ccttccctgt gtgtccaccg atcggatggt ggctctctcg aaggccggcg
     5161 ccggcgccga cctcaaacgc attccgcccg acgtgttgcg gtcgaccctc accgagttgg
     5221 cgtcggccgg ctacgagacg gtgggcctca cccccgtcga gtcgacgtgc ctcgccgagc
     5281 gggtcgtcgg tacctacacc gacgccgatc tggccgaggt gatgacgacg ctcagcctgc
     5341 cggctgatcg agcggtcacc gcccttccga cctgtgtgac cggcgaacgg gcgaacgaac
     5401 tcggagcttg agggtctcgg ccactaccgt tgctggccat gttggacatc cgtctcttcc
     5461 gcaacgagct cgaccaggtc agggcaggtc tcgcccgtcg cggcgagagc atggactcga
     5521 tcgacgacgt catccgtctc gacaccgagg tacgcgaggt caacacctcg cgtgacgatc
     5581 tgcggtcacg aatccgggcc atctccggcg aggtcggcca gctcatgcgg gacgggctca
     5641 aggaggaggc cggcgagctg caggccgaga gccggtcgct cggcgagcgg gagaaggagc
     5701 tcgacgccca ctcggccacg gtggcggccg agctccgcga tctgctgctg cgcctgccga
     5761 acctccccgc cgacgatgct cccgacggcc tcggcgagat cgacaacgtc gtcacccgca
     5821 tcgagcactt cgaccccgac tcctaccagg accaccagcg tgtggcgcac tgggacacgg
     5881 gcgaggcgct cggcatcctc gacgtcgagc gcggcgtgaa gatgtcgggc tcgatgttcg
     5941 tcatgtaccg cggttcgggg gcgacgctcg ttcgggccct ctgccagctg gcgctcgacc
     6001 gcaacgcgga cctctacgaa gaggtccgtc ccccgacgct cgtgcggacc gacaccatgg
     6061 tgtccaccgg ccacctgccg aagttcgccg aagacgcgta ccacctcgaa cgcgacgacc
     6121 tgtacgccat cccgacggcc gaggtaccac tgacgtcgct ggcccgcgac gagatcctcg
     6181 acgaggccgt gctcccgatg cgcctcatgg cccacacctc gtgctaccgg cgcgaggcgg
     6241 gctcggcggg caaggacacc cgcggcctgc tgcgcgtgca cgagttcgac aaggtcgagc
     6301 tcctggcgta cgcgaccgcc gaccaggcgg ccggcatcca cgtcgagatc ctcgcccggg
     6361 ccgaggcgct gctcaccgac ctcggcctcg cctaccgggt gctcgacctg tgcgccggcg
     6421 acctcggcaa ctcctcgcgt cgcacgttcg acctcgaggt ctacgccccc ggtgtcgacc
     6481 gctggctgga ggtgtcgtcg gtgtcgtggt tcggcgacta ccaggctcgg cgggccaaca
     6541 tccggtaccg ctccgccggt ggcaagggca ccgaggtgtg ccacacgctc aacggatcgg
     6601 cgctcgccgt tccccgggtg tgggcaggca tcgtcgagac acaccgacag cctgacgggc
     6661 gcgtgctgat ccccgaggtg ctccgtccgt acatgcgtgg cctcacgcac ctcggctgac
     6721 atgtccggcg tcgacccgga gttcacccag ccctccttcg agggcgagcg caacaactac
     6781 gacgtcggtg gtctcgacga ggctgctgcc gccgccgacc cgttcgtcca actgcggcgc
     6841 tggctcgacg aggcggccga agcgcaggtt cgcgagccca cggcgatgac ggtgtcctcg
     6901 ctgggcatcg acgagcgcgt ccattcgcgc accgtcctgc tccggcagct gacgtcgtcg
     6961 gggctcgtct tcttcaccaa tcgcgacagc cacaagggcg agcagctggc cgcgcacgcc
     7021 ctgtgtgcgg cgcaactgct ctggctcgac ctgcaccgac aggtgcgcgt cgaggggatc
     7081 gcccggctgc tcgatgaccg ggtggccgac gactacttcg ccggccggcc ccgcgggagc
     7141 cagatcgggg cctgggcatc gccgcagtca caggtgctgc gcagccggga ggaactcgag
     7201 gcgagagtcg aagcggccga gcggcagttc gccgacgtgg acatcgtccc gcggccaccc
     7261 aactggggcg gctactgcat cgaacccgac tacttcgagt tctggcaggg ccgaccgagc
     7321 cggttgcacg atcggctgcg ctacgtggtc gggcccgacg gccactggaa acgcgaccgg
     7381 ctcgccccgt agatcagccc gttcagagca cggggcggcg ttcgccgtcg ctgccgcaca
     7441 cccacgcgtc tcggggccag atccagtcgg cgccgccgtg gccgtggatc tggacgccgc
     7501 agaacacggc gacatcgtga tgctcccaga tccgcgcttc gtcgagggct ttccacaagt
     7561 cgacctcgac ggcttccagc gtggcgatcg tcaggtcgac gagcgacacc ggcggatcgg
     7621 ccgggtcgac gagccgaagg gccagccggg tcgcctcgta gtcgtcgagg tcgatctcgg
     7681 tcggcaacgt gccttcggcg ctgcgctggt ggatcgccgc caacgctccg cacgtgttcg
     7741 tcgcgaccga ctggccgggg cggagtgtga cgccgacgga gccgtcgtcc tcgatgccga
     7801 tgtggggaaa gcccagcacg agcaccaggc cccggcccgc gtcatcgggg atgtggctga
     7861 acgccgcacc ccagcccgtc cggccgaggg cagggacccc tcccagaccg gcgagcgtga
     7921 atgccgggcc ccagatcgcc tggatgcggg cgaagaagtc cgtcgtgagc tcgtcgcggc
     7981 agatgctgat gagggggagc gtgttgccca ccttgaagcc cagctcctcg gtcgaggtcc
     8041 agacctggtt gaggaacacc ccgctcggga cggcgcccgg gaagtgtcgt tcgagggtgt
     8101 cgtcgaagcc gctcatgggc gccaagtctc gctagtcgcc ccgtccggtg ccaaccctgg
     8161 agcgacgaag gaccacggtg atggtggtcg ccgcgaccag gatgatcgcc acggcgatac
     8221 cgacgcgttc ctcgccgacg tcggtgatcc agcgcaccag accgtcacgc catcccgcga
     8281 tcgtctcggc aaacgcgttg tcggccgacc cgcccgtcag gatccgcgcc tcgtaccagc
     8341 cgtagtaggt cgcgaccagt ccgctgacga tcaggagcac accgctgatc cgacccatgt
     8401 gggggagcag gcgccggagt cgcgtgacca caccgtggtg ggcgaagctc accgccagcg
     8461 tgagcacggt gatgatcgct cccatgccga ggccgtagat gacgagcgtg ccgagaccgg
     8521 tgaggaagtc gtgacgtcgg aacgtggtgg tcacggtgcc gatgaacggg ccgatcgtgc
     8581 agctgagcga tgccacgccg taggacaccc cgaagacgaa cgcggaccac gccgactgcg
     8641 atcccgatgt cgtcgccagt ttcggaacgc ggatcatcgg cacgtagccg gccaccatgg
     8701 cgatgccgag gaacaccagc gcgatgccca ggacgacgat cacccagggc aggcgctggc
     8761 cgaccacttc ggacacgccg ctccagacgg cgccgatgag gccgaacacc aggacgacac
     8821 ctgcggtcat cgcgccgctc acggcgagcg agcggccgag ctccccgcct cggggtggtg
     8881 catcggcgtc gccgctgctc tcgagcccga ggtagtaggc gaggtacgcc ggcaacagcg
     8941 cgaagccgca gggattgacg gctgccacca tcccggcgga gaaggccaac agcagggtcg
     9001 ccgtgctcac cgtcgcaccg attggtcgtt cacgacgcag cgtcgatgag gtcgcgaagt
     9061 acctcgtcgt cggtgatcgc ctcgttgtgc aacgcggaca cggtgccgtt ggccgtcagc
     9121 acgacggtgt gcggcaactg caccccaccg aaggtcgtga gcacctcgtt ggtggggtcg
     9181 acggtctggg ggtacgtcac cccggtccgt gtgatcatgt cgcggccgag gtcgggtgat
     9241 tcgaacacgt cgacgccgac gaagttcacg tcgctaccca gctcggcgta ggcccgctcg
     9301 atcaacggca tctccttgat gcacggcacg cagctcgacg cccagaagtt gatgacggtc
     9361 ggcttgccac gcagcgacga cagggcgacg tcggcaccgt cgaacccctt cagggtgacg
     9421 tccggagcga tcttgccgac ctcggctgtc ggcccaccgt cggagggggc gcgggcatcg
     9481 ctgttcacga ggtcgtcgac gccgatcgcg ctgttgtcgt ccaacgcggg gcggagcaca
     9541 acgagccagg agacgaagag tgcgagcgcc gctgcgagaa ggatcgcccc gatgaacttg
     9601 tgcggagatc gggctctcgg cggcgcgttc tcgggctcgg tgctcacgcg ccaacggtag
     9661 ctgtcgggcc cgaccggagg cgatcgcgga cccggctccc gacgcggcac ggaccccggc
     9721 ccgtcggccg aggtccgtgc cgctgtgtcc cggatcggga gctcgctgtt gatcacgctc
     9781 gagggttcgt cacgtgatct cgcatcgccg tgccagaccg atccacccgt cggtcaggcg
     9841 ggctggagat ggcggaggtg ctccgggaag gggatcaccg ggagctggtc ctccgggcgg
     9901 gggtgcttgg gcggacgccc ccgacgacgc ttggtcatga cgatcttgcc cgagacgaac
     9961 agctgaccac cccagacacc ccactgctcg ccacgagcga gggccgtgtc gaggcactcc
    10021 acgatgacgg ggcaggtgga gcagatgcgc ttggcatcgg cgatgtcggt gatctcgtcg
    10081 gagaagaaga ggcgagcggt ttcagtgcca ccgtcggcgc acgcggcgtc gcgttcccag
    10141 gcggtgtcgg gctcgagttg gatggtctgg gcagcagtca tgtcagtcgt tctttcgtga
    10201 tccgggcgtg gcccgggtgg tgatcaggtt ctgtgtcggt cgcggcggtg gggcgtgcgg
    10261 tgcgctcggg tcgtgagcga ttgacgggtt cggtgattga tgttcatcgg ttccttcttg
    10321 ttggaggccg ctccgggatg gggcggtggt tgggtgggac aggtggcgtt cccgatcact
    10381 ggccgggaac gacgaagggc cgccggtttc ccggcggccc ttcggagatc tgcttcgttt
    10441 cggtgagtcg aaactctacg aagatcctcc tgggccgctg tgcggggtcg ggttgatcgc
    10501 ggcgtgctcg tacttggtcg ccgcatcgaa ccacttgccg gtcgagatgg acgcgaggca
    10561 ccgctgcatg gacgcgcgca gcgaggtggc ggtgagcact acctcgttgg agatcaagat
    10621 ctcgttgcgc atgggactgg ttgccttcgg tcgggactcg tcggaccggc tggtcgagag
    10681 gagttggtct cgattcgatt cagtatgccg ccggcggacc ggcgtgcggt gtcaatgaca
    10741 catccatttc gcggggccgt caaatgatta aacggaagat cgactcagcg ctcgatggcg
    10801 tagtggagtt ggccgtcgac ggtgcgtgac gtcgcccggc cggcgagacg gagatgttcg
    10861 aggtgggcga acgtctcgga ttcggccatc gcgccccacg accgctcctt gaagaggtgg
    10921 tgggagaact cgatgacgct ggcgtcgccg atgtcggcgc cgatccgggc gaggtcgtcg
    10981 agacgctcga cgtggtgccg ttcgatggcg tcgacccgac ccacgaggtc ggtgaagggg
    11041 tgcccgtgag cgggcaacac gagctgcacc ccgtcgagcg ccgcgacctt gtggagcgcg
    11101 gcgaagaagt ccgcgagcga atcgcgcgag tgggtcatcc cggagatgtg cggggtgatg
    11161 gtgggcagca cgtggtcacc ggagagcagg atcccgtccg ccggatcgaa caagcacacg
    11221 tggtcgccgg tgtgacccgg ggtgtgcacg gcctgccact cgcgacctcc gagcacgatc
    11281 acctcgtggt cggcgacccg gacgttgggc tcgttgtcga cgaacccgcg cttgccgtcg
    11341 tcgtcccagg cgcgcatcca cgtgatctcg tcgctcgtcg ggaacgggaa cgtgccgccc
    11401 cagggagagc ggcgagtggg gagctcgggg atcggaccgt cccccagcaa cgcgtcggcg
    11461 aaccgggccg ggtcgaaccc gtcgttgctg cggcgcccct cgagctcgac gtcgtggttg
    11521 tggtcgtcgt cacggttgaa cagggtggtg aagtgttcgt gggccacgat gtcggcctga
    11581 ttggtgatgc gcagtcggcc ggcgccaccg aagtggtcgg ggtggctgtg cgtcacgaag
    11641 accgtgtgga tgcggcggac cggtgcaccg atctgcgcca tccggtcggc gagcgcctgt
    11701 gcggtgccgg ggtcggggaa cccgggatcg acgaccgtga accccttcga gtcctcgagg
    11761 gcgaagcagt tgacgtggcc cagccccggc aggttgatcg gcaactgcag gcggtagatg
    11821 cccggagcga cctcggtgac gtcctccgat gcgggcagct tctcctgccg ttcgatcggc
    11881 gccgactcgg tggtccttcg cgtcgagctc acgccgacgg cgttcccgga gcgtcggtgg
    11941 cccggaacag cgtcgaccgc cagtacgcca gctcggcgac gctctcgcgg atgtcgtcca
    12001 tggcgcggtg tgacgttgcc ttgcgcggtg ccgccgccaa cgcctcggga taccagcggc
    12061 ggccgagctc cttcagtgtc gacacgtcga tggatcggta gtgcagccac tcctcgatct
    12121 cgggtaggta cgcggcgagg aagcggcgat ccatgccgat cgagttgcca cagagcggaa
    12181 cggtgccggc cgttccgacg tgctgcttga tgaacgcgag cgtctgggcg ccggcctcct
    12241 cgagcgtgat ggtcgacgcg gcgatctggt cgagcaggcc cgagctggtg tgcatgtcga
    12301 cgacgacctt gtccatgttg gccagggcct ccggcggttg atggacgacc aggtccggac
    12361 cctccgcgac gatgttgagg tcgtcgtcgg tgaccagggt cgcgatctcg acgatgacgt
    12421 cggtccgcga gtcgaggccg gtcatctcga gatccatcca caccaacatc cggttgagcg
    12481 taccggcgtc gggccacggt aggtttcgcc cccgtggaga taccgatccg acgcaccgat
    12541 ccgaccgacc agaccctcga gctcccacgg gtggcgaagc ccggtgacgc cggcgtcgac
    12601 ctccgctcct cgatcgacgt caccatcccg cccggcggcc accgcgccct cgtgcccacc
    12661 ggcatcgcgg tcgcgattcc ggcgggttac gccggcttcg tgcagccccg cagcggcctg
    12721 gcgctcaagc acggcgtcac ctgtctcaac acgcccggtc tcatcgacag cggctaccgg
    12781 ggtgagttga aggtgctcct gatcaacacc gaccccaccg acgcgttcga aatcacccgc
    12841 ggcgagcgca tcgcgcagct cgtcatccaa gcggtcgtcg agccggtgtt cgtcgaggtc
    12901 gatgaactcg acgcctccga gcgcggcgag ggcgggttcg gccacaccgg tcggtagctg
    12961 ggtcgggctc agacgtccga actcaggggc acgtggagtg acggcagcag gcggtcaccg
    13021 tcgggctcga gcacgtaccg ggcgagacgc tgaccgctcg gggtgatgac gatcgcctcc
    13081 ggaggggtgt ccttgccggc catcgcccag ccgacgagtc ggacctgcgg gtcgccgccg
    13141 tcggcggtcg cggtgccatc ccactcgatc gccgtgatga cgccgtcggt gacgccgctt
    13201 ccggagttca cgtaccgatc ccagcgttgg cccgtccggg agaccggcgc cgacaccggg
    13261 aagtgggtgt ggccgaggat caaccagagg tcgtcgaggc cggccgtgcg gacggcctcg
    13321 cacagcagct cctcgtcgag ggagtcgtag gacgagttgg cgccgaacgt cgggttcacg
    13381 gcgatgagcc gatcggggat ctcgcccgcg agcatctgct cctcggcccc ggccgggggc
    13441 agcccttcgg gggtctcgag acggggcacg tcgatgagcg tgttcgcgat ccaggccgac
    13501 agcttcccga ggttgtcgcg cccgggggcg ttccagccgt cggtgtggtg gccgtggcag
    13561 atcacggcga gcagctgccc agttggggtg cgcagggcga agtagtcggc gagaggaaag
    13621 gtgcccaggt gctcgcgcag acggtcggcg accatcggcc ggttgttcgg gttgtcgtgg
    13681 ttgccgaccg tgcggaagta gcggccgccg tgaacgaagt ggtcgcggat ccgtcggtag
    13741 atgccagggt tgttgcccac gatctggtcg aggtgggtct tgtacgtctc ggtgaggagc
    13801 gcagtgtggc tgtaccgggc catcgccgcg ccgagcatgc ggagcaggtc gtagacggca
    13861 ccgtacgtcg agccgccgac gatccagtag tcctcgatgt cgccgttctc gcacagactc
    13921 cagtcgtcgt cggcgtagta gtcgaggatc agctcgtaga ggtgtttggt gcgttgccgg
    13981 gcgggccagt cgaggcgacc ggggacacac cggtggaggt cggagaagac ggcgagacga
    14041 acgtcggcag ggatgtcgag cacgcggttg gacggtggcg cgggggtgcg accggcggcg
    14101 atggcgcgca tctgctgggg gaactccgcc gtgagccgtt gcgcccgccg gttgttggtg
    14161 gcggcccgca ccatgatcgc cggggcccag atcggcgtca ggatgccgac gatgccgagc
    14221 ttgatcgtgg cccagacact ggcgatccag cccacgtgac gggggtcgag cgctgcctcg
    14281 tgacggtcgg ggtcgcgggc caacgcccag cgcgtcgctc cggagacgat ccggctggcg
    14341 cggtagcggc tgcgggcgga cacggccgct agtttctcac ctctgggtgc gggggacgac
    14401 caccgggcgt tcgccggtcc gcccgggaca ggagtcgacg atgacggtca acgagcgagc
    14461 gcccgagggc gagcggaccc tggaagcggt gcgtgagcgc ctgtcgatgc cgctcgacga
    14521 ggcgatgatg acgcaacggg ccgtccgtcg cgtgctgccc gatcccgtcg acgacgacct
    14581 ggtggtgcac ctcatcgagc tcgcgcttcg tgccccgacc ggctcgaacg gtcagaactg
    14641 ggagttcgtc gtggtgaagg acgcggcgac gaaggcgaag ttcgccaagc ggtaccggca
    14701 gtcgtggtcg atctacgggg ggatcggtcg gcgcgtcagc aagggcaatc cgtcgatgct
    14761 caaggtgctc gacgcggtgc agtggcaggt cgatcacttc gaggaaatcc cggtgctcgt
    14821 cgtgccgtgc ctcaagggca accacgcccc cttcgtcccg atgccgccgt tcgctccgtc
    14881 gtcgtactac ggctccatct acccgtcggt gcagaacctg ctgctcgccg cccgttcggt
    14941 cgggctgggg gcctcgctca tcacgcttcc cctgtggtcg tccacgtcgg cgcggaagat
    15001 actcgggctg ccgatctcgg tgatgcccgc gtgcatcgtg ccgctcggct ggccgccagg
    15061 ccgttacggc cccaccaccc gtcgcccggt cggcgacgtc acccac
//
LOCUS       DAOSOA010000033        15100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_3971_length_15100_cov_8.030508, whole genome shotgun sequence.
ACCESSION   DAOSOA010000033 DAOSOA010000000
VERSION     DAOSOA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15100
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3971_length_15100_cov_8.030508"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKY13_03845"
     CDS             complement(<1..329)
                     /locus_tag="PKY13_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOT79091.1"
                     /translation="MTEATAPMERLAAEASILLCCGSGGVGKTTTAAVVALEAARLGR
                     RAVVVTIDPAKRLADALGLGELTNTPSLIDGPWPGELSALMLDTESTFNGLVARYAGS
                     ADQAEKIL"
     gene            complement(326..1303)
                     /locus_tag="PKY13_03850"
     CDS             complement(326..1303)
                     /locus_tag="PKY13_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOT79092.1"
                     /translation="MDPAQFLTAPRVTIVAGKGGVGKTTVTATLAMAAAHLGLRALVI
                     EVEGKSGLPALFGKERLDYEEQVLFTADGPHGAGEVRARTLTPDESLVEWLRDHGLKR
                     ISKRLGESGAIELIATATPGIKDVLILGKVKQLESRGAADVIIVDAPAAGHAITFLSS
                     AQGMLDAVTVGPIEAQAREVRNLLTDASRCQVVLVTLPEETPVNEVIETAYSLEDRIG
                     IKLGPVVVNGIYPDLPGLDQSPTAAADAEEVELSTELAAALEAAATFRRDRQDLQRHQ
                     LSRLAKDLPLDQVRLPFVFRSDLGLDSLRALSESLVEQLGRLDAVGSPS"
     gene            1351..2640
                     /locus_tag="PKY13_03855"
     CDS             1351..2640
                     /locus_tag="PKY13_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT79093.1"
                     /translation="MISTIAGTLTLIGATPASADTEATCPNPKGCVRAVTVNGFIDEI
                     NVDFMERAVSEAEAVEGYRAVILVLDSPGSVVEASVLQQLVDRLETAEVPVSIWVGPS
                     GAQAFGGAAELVTALPSTMAPNTDIGEVGDPRLANSPVNDPASEFARLRTKVLDEKAA
                     TKAGIVDRIDPTLGDHVLGIEGMPFKQVKDDEGRDKREIAVDGVTQKLPVTIQLLHTA
                     ASPGVAYLALGIAIGLLLFEFFTAGVGVAGVVGAASAVMAAYGLAEIPVRPWALALCL
                     FSAFAFAIDIQTAIPRTWTGIGLVSWTVGSVFLFDGFRAPWLALIAGIGGMAVAMLSG
                     MPAMVRSRFATPTLGRDWMIGREGAALTDVDPEGTVEIDGGQWRALTNRATPVLAGEA
                     MRVVAIDGLTLEIEPLEGGAKDYREQRGGRRKSASDS"
     gene            2688..2990
                     /locus_tag="PKY13_03860"
     CDS             2688..2990
                     /locus_tag="PKY13_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT79094.1"
                     /translation="MVRREAQGGNLSAQPSFESWQHRGACRGPHATVFFPPPQFERKH
                     ERAERELRAKEICSGCCVATDCLDYALEIREPHGIWGGLNENERRVILEARSARVS"
     gene            3061..3627
                     /locus_tag="PKY13_03865"
     CDS             3061..3627
                     /locus_tag="PKY13_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HOT79095.1"
                     /translation="MFVVILGLLLMGVIEIAVMVAVSNALSWEVALLAIVLAGAVGAW
                     VVKREGTATWRRVMSGVRAGQMPTSSVLDGCLVVVAGVLLLVPGFVTDVAAMALALPP
                     VRRLVRVRATDHFQRRIAAQVSRARTTTTVFGFGDPMSYGGFGGPGFGPGGTRRSNRD
                     DDIIDLDAEEVFLDETIAEIEPPREWPA"
     gene            3624..4463
                     /locus_tag="PKY13_03870"
     CDS             3624..4463
                     /locus_tag="PKY13_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79096.1"
                     /translation="MKMHLDVDRPEEVPIRDAATVMLVRDGVDGVEVFMLRRNLNSDF
                     VGGAYVFPGGGVDAGDGAAEVGDVCVGRADADASALLGIESGGLAYWVAAIRESFEEA
                     GLLLAVDDNDQMVRFDDPTVNERFVEHRRAVDAGEVSLVDIARSEGLRLATDRIHYFS
                     RWVTPLGAPRRYDTRFFIAHPPEWQNPVHDDREVIANLWIRPAEALDRHARGEFELIF
                     PTVRSLEALGRFDTAEQIVRHASEIAHIEPILPTIVDGDHGLRIVLPGDGSYDALTSK
                     RLD"
     gene            complement(4488..5714)
                     /locus_tag="PKY13_03875"
     CDS             complement(4488..5714)
                     /locus_tag="PKY13_03875"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOT79097.1"
                     /translation="MSEPTLPTSEDVIGLLRGVIDPELGSDIVTLGMAKGATVSPDGF
                     VRITVALTTAGCPLRAQIQRDVRDRVGSLPGVTKVKIDWTELTAEEKATTMATARRNI
                     AERAPVTAIPATTKVLMIASGKGGVGKSSVTVNLATALAATGFKVGVIDADIWGFSVP
                     RMLGVDGRLEAGEADGRIAPHRQTVGRGELRIVSMGFLVDDEETALMWRGLILQRAVR
                     HFLEDVDWGHDLDYLLIDMPPGTGDVQMGLAKLLPRAEMIVVTTPSLSAQKVAVRAVN
                     MGRQNYLRIIGVVENMSEFVTPDGERFAIFGTGGGQRLAKTIGAPLLARIPIENAVAE
                     GGDTGTPIALGDGAAAEAFRELAHLLSTDIAPPVDLAGCSVRLLDAVNAALDAHDSGA
                     NDSDDDTKVASPVAGG"
     gene            5677..6198
                     /gene="erpA"
                     /locus_tag="PKY13_03880"
     CDS             5677..6198
                     /gene="erpA"
                     /locus_tag="PKY13_03880"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOT79098.1"
                     /translation="MTSSLVGRVGSLTSPFYGGRAPRPRRACATGGGGIGPRGTLLHG
                     ALWVDRRSGGTLTVASRHQEAQHMITLTDSAAVKVRQLIESEGEENLALRVAVRPGGC
                     SGFSYEMFFDTDIAADDLSSEFDGVKVVVDPSSAQLLTGAELDYKDSLEKSGFEINNP
                     NAQKTCGCGSSFS"
     gene            6219..7769
                     /locus_tag="PKY13_03885"
     CDS             6219..7769
                     /locus_tag="PKY13_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT79099.1"
                     /translation="MQFQLNGDQVEVADDGCSLLDVLRDGFGLTSVKDGCSPQGQCGC
                     CTVLVDGEPRVSCVTPARRIADRRVDTLEGLAPEVALAWGEAFCATGGSQCGFCTPGI
                     VMRLEGLRRKGGDLGERPAVDRALAAHLCRCTGWQTIVEAAAAYGTPTSDDRDLAAAG
                     RRASIEGGEPQRVAPEIALGRGGFADDGAPDDAVIGVVDATSGWQVAPTMAEARRQVG
                     KVQGRRSTIDATMPVDLPDGDWAVELQTGWVDPAYLETDASWCAPGGEPANPLANGGA
                     FGAKHTSPVPGDARRLADELGTPVRLRWSREDVVRNGPKRPPVAIGLRSDGGGVVRVA
                     RTPGLVDRIAAFAPELTVEEVDVVGPATSADARAAGWAEVAVAMAALDAVPGTPVEIT
                     SPSGGWASVTVDEQGAHVRVRCGAVLDAVVLRSYCIGAVHMALGWVTSEGLTVDADGS
                     VASLTVRSFGIVRPGEMIPVEVEIIDEPGVEPVNGSDAVFAATAAAVWIAQGTPPAWP
                     TGRPVTSV"
     gene            7797..8147
                     /locus_tag="PKY13_03890"
     CDS             7797..8147
                     /locus_tag="PKY13_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOT79100.1"
                     /translation="MAPPVGPYTPLVRAGDFLIASGQLGLGDAGLVDGGVAGQLRQAI
                     ANLAALLESAGASLSDVVKTTVFLVDMDDYAVMNDVYCDGFGDHRPARSAVAVAALPL
                     GAVVEIEAWSHVPA"
     gene            8197..8355
                     /locus_tag="PKY13_03895"
     CDS             8197..8355
                     /locus_tag="PKY13_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79101.1"
                     /translation="MLGAILIIIALLVVVPVGVLISGGALAGIIGFFVQKDVDQLNEG
                     SELIDLNG"
     gene            8444..9352
                     /locus_tag="PKY13_03900"
     CDS             8444..9352
                     /locus_tag="PKY13_03900"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOT79102.1"
                     /translation="MPERSLGDVAHDRYGLVTDRELDELGITEGERRRRIAAGEFERL
                     NGKVLAVAGSPRTWSQRAAAALLAVPSGVLGYGSAARVLGLPEYRDHDEVTLSVPLAA
                     HYFIPGIEIRRTGRWSDEHVMVTDGLRHTTLGRTLVDLALVTPDRRLQDVIEEAVSSQ
                     RITWDELEATYRSLAGQGRSGTAGARRVLEAVEGRPPSEAELERMYTELLQRAGVPVP
                     TMRMTPPWAERQPGRVDAMYEDATAIVELDGRRFRVRHAALTEDARRDRLAREHGYDT
                     VRFTHLQLRNDPDHVVEVSRTLSART"
     gene            complement(9374..9997)
                     /locus_tag="PKY13_03905"
     CDS             complement(9374..9997)
                     /locus_tag="PKY13_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT79103.1"
                     /translation="MRSLDMAGYSWATATDEASVVAGSWAGAIVDASDDADGAFTFAR
                     MLGRGDLGIAPLLLLVRGSQLGELESRHELFEDFCVTPFHPMEFEARLKHLLTRQGVL
                     ADDSETIVYKALVLNLETYQASIDDDPLDLTYMEYELLKFLAQNPGRVFTRETLLSRV
                     WGYEYYGGARTVDVHIRRLRAKLGEQHAQMISTVRSVGYRFGESRWG"
     gene            complement(10056..11393)
                     /locus_tag="PKY13_03910"
     CDS             complement(10056..11393)
                     /locus_tag="PKY13_03910"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOT79104.1"
                     /translation="MEHQQSYVLRTVEERKVRLIRLWFCDVLGQLKSVAISPVELESV
                     FEDGIQFDGSAIDGFSRVQESDVLAIPDASSFQLMTWGDGAEVSARMFCDLVNLDGSP
                     FEGDPRQVLKRNLQRAHDAGFTLMCAPEVEFFYLESDDPSQAPVPLDTASYFDLTTKD
                     VSSDIRRATIQRLEATGIPVEYSFHEDAPSQHEIDLRYTDALSMADNVMTLRLVVREV
                     ALQHGVHATFMPKPIAGVQGSGMHTHISLFAGETNAFYEEGAESNLSPVAQQFIAGLL
                     RHAPEITAVTNQLVNSYKRLVSGFEAPVHVSWARNNRSALVRIPVTKRGKEAAGTRIE
                     YRALDPACNPYLAYSVLLAAGMAGVDGLYELPPEANANLFELTEAERVKADIPLLPSS
                     LSDALDVMEASDLVREALGEHIYEWFLRNKRAEWSDYKAHVSQFELDRYLKNW"
     gene            11555..12574
                     /gene="glnII"
                     /locus_tag="PKY13_03915"
     CDS             11555..12574
                     /gene="glnII"
                     /locus_tag="PKY13_03915"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HOT79105.1"
                     /translation="MPYRAEYIWIDGTEPTPLMRSKTKIIENGKEPGIWGFDGSSTNQ
                     APGSDSDCVLNPVFQCPDPTRGEGDILVLCEVLLPGTLEPHPTNHRRACLEAHEKYAK
                     DFEPMFGIEQEYTLFQEGRPLGWPKEGYYYPAPQGPYYCGVGSVEIAGRDLVEAHTTA
                     CIQAGIGIVGTNAEVMLGQWEYQIGILNTIDVADHLWMSRYLLYRIGEDFGVNASISA
                     KPIKGDWNGAGCHTNFSTKQMREGWDPIIAACEALGGPGKVEEHLAGYGHGYEERLTG
                     LHETAHFSEYRYGVSDRGASVRIPWQVGVDKKGYIEDRRPNANIDPYIVSRLITNTVC
                     EAASA"
     gene            12694..>13141
                     /locus_tag="PKY13_03920"
     CDS             12694..>13141
                     /locus_tag="PKY13_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOT79106.1"
                     /translation="MSSVRVALAQLNPTVGDLVSNTDAILEALSESEAGGARITVFGE
                     LAVCGYPPEDLVLKRRFVDDCWTQVERIAEASGSCAAVVGVPQRAEDGRLYNAAVVCR
                     DGRVDYVYRKQELPTYAVFDEHRWFRPGDVDQPLWDFDGVPVGISVC"
     assembly_gap    13142..13241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13242..14474
                     /locus_tag="PKY13_03925"
     CDS             <13242..14474
                     /locus_tag="PKY13_03925"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOT79107.1"
                     /translation="VDQPLWDFDGVPVGISVCEDIWIPDGPVLRQATAGARLLLNLNG
                     SPFHHDKIVHREKMLRDRAVAVAAPIVYVNQVGGQDELVFDGGSFVIGGDGALVARLP
                     QFRTHLEFVDVELDGASAPGDLVVPRLESAEEMWSALVLGTRDYTIKNGFTDVVIGLS
                     GGIDSSIVAAIAVDALGADRVHGVMMPSRYSSDGSITDAERLCNTLSIESRVIPIEAG
                     HRAFEEMLAPSFEGIAPDLTEENLQSRLRGVTLMALSNKFGWLVLTTGNKSESAVGYS
                     TLYGDTAGGLAVIKDVYKTVVYDLCRWRNDEAGHEIIPESVLTKAPSAELRPDQRDDQ
                     SLPAYEVLDPILRGYVDGDQTVTDLIEAGHDPAVVGRICRLVDIAEYKRRQNPPGIRV
                     TSKAFGRDRRLPITNRYR"
     gene            14565..>15100
                     /locus_tag="PKY13_03930"
     CDS             14565..>15100
                     /locus_tag="PKY13_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79108.1"
                     /translation="MLRSLALAFCDVGAGVLALAGLIKFFDPTPWVTSLGALGVPLGP
                     VRFRRSVVAAARLVGVSEVAIAVLVFAVGGTLALGLLAVAYAVFVVVSVVATRRGGAS
                     CGCFGVRSAPISSLHVGLDAVVAAAAVVGAVDGADALAVRLGDGVGATVAYLVFLGLG
                     VAAMVTVMTTGAELWALKR"
BASE COUNT         2221 a         5022 c         5258 g         2499 t
ORIGIN      
        1 aggatcttct cggcctggtc ggcggagccg gcatagcggg cgacgagccc gttgaacgtc
       61 gattcggtgt cgagcatgag cgccgacaac tcacccggcc acgggccgtc gatcagcgac
      121 ggtgtgttcg tgagctctcc gaggcccagc gcgtcggcaa ggcgcttggc cggatcgatc
      181 gtgacgacga cggcgcgccg tccgaggcgg gcggcctcga gggcgaccac tgctgcagtc
      241 gtggtcttgc cgacccctcc ggagccgcag cacaagagaa tcgaggcctc ggccgcgagg
      301 cgctccatcg gcgcggtggc ttcggtcatg acggcgaccc gaccgcgtcg aggcggccga
      361 gctgttcgac gagcgactcg ctcaacgccc ggagcgagtc gaggccgagg tccgaccgga
      421 acacgaacgg gaggcgtacc tgatcgagcg gaagatcctt cgccagtcgg gacagctgat
      481 ggcgttgcag atcctggcgg tcgcgacgaa acgtcgcggc ggcttcgagc gccgcggcca
      541 gctcggttga cagctcgacc tcctcggcgt ccgccgcggc cgtcgggctc tggtcgaggc
      601 cgggcaggtc ggggtagatg ccgttgacga cgaccggtcc cagcttgatg ccgatccggt
      661 cctcgaggct gtacgccgtt tcgatcacct cgttgacggg tgtctcctcg gggagggtca
      721 ccaacacgac ctggcaccgg gaggcgtcgg tgagcaggtt ccgcacctca cgcgcctgcg
      781 cctcgatcgg tccgacggtg acggcgtcga gcatgccctg ggcgctgctg aggaaggtga
      841 tggcgtggcc cgcggcggga gcatcgacga tgatcacgtc cgcggcgcct ctcgactcga
      901 gttgcttcac cttgccaagg atcagcacgt ccttgatccc cggggtcgcc gtggcgatga
      961 gctcgatggc cccactctcg ccgagacgtt tcgagatgcg tttgagcccg tggtcccgga
     1021 gccattcgac gagcgattcg tcgggcgtca gcgtgcgggc tcgcacctcg ccggcaccgt
     1081 gggggccgtc ggcggtgaag agcacctgtt cctcgtagtc gaggcgctcc ttgccgaaca
     1141 gcgcgggcaa cccggacttg ccctcgacct cgatgacgag ggcgcgaagc ccgaggtgtg
     1201 cggcggccat cgcgagggtc gccgtcacgg tcgttttgcc gaccccgccc ttcccggcaa
     1261 cgatggtcac ccgtggtgca gtgagaaact gcgcaggatc cacttttctc ctccggaggc
     1321 cttcgggtgc gcaggatagt cagatgggcg ttgatctcaa ccattgcggg cacgctcacg
     1381 ctgatcggcg cgaccccggc gtcggctgac accgaggcta cctgccccaa tcccaagggc
     1441 tgcgtccgcg ccgtgacggt caacgggttc atcgacgaga tcaacgtcga cttcatggaa
     1501 cgcgcggtca gcgaggcgga ggcggtcgag ggctatcggg cggtcatcct cgtgctcgac
     1561 agccccggct cggtcgtcga ggcgtcggtg ctgcagcagc tcgtcgatcg tctggagacg
     1621 gcggaggtgc ccgtgagtat ctgggtcggc ccctcgggcg cgcaggcatt cgggggtgcg
     1681 gctgagctcg tcacggcgtt gccgtcgacg atggcgccca ataccgacat cggcgaggtg
     1741 ggtgatcccc gcctcgccaa ctcgccggtg aacgacccgg cgtcggagtt cgctcgcctg
     1801 cgcacgaagg tgctggacga gaaggccgcc accaaggccg ggatcgtcga ccggatcgac
     1861 ccgacgctcg gcgaccatgt gttgggcatc gagggtatgc cgttcaagca ggtgaaggac
     1921 gacgagggcc gcgacaagcg ggagatcgcc gtcgacggtg tcacgcagaa gttgccggtc
     1981 acgatccagc tgctgcacac cgcggcgagc ccgggcgttg cctaccttgc actcggtatc
     2041 gccatcgggc tgctgctctt cgagttcttc accgccggcg tgggtgtcgc cggcgtggtc
     2101 ggagccgctt ccgccgtgat ggcggcatac ggcctggccg agatcccggt gcgaccctgg
     2161 gcgctggccc tgtgcctctt ctcggcgttc gcgttcgcga tcgacatcca gacggccatc
     2221 ccacgaacgt ggaccggcat cggactcgtc tcgtggaccg tcggttcggt cttcctgttc
     2281 gacgggttcc gtgcgccctg gctcgcgctc atcgccggta tcggcggcat ggcggtagcg
     2341 atgctgtcgg gcatgcccgc catggtccgc tcccggttcg ccactccgac gctcggccgc
     2401 gactggatga tcggacgaga gggcgcggcg ctgaccgacg tcgatcccga gggcacggtc
     2461 gagatcgacg gtggacagtg gcgagccctc acgaaccgcg ccacgccggt gctcgccggt
     2521 gaggcgatgc gggtcgtggc aatcgacggg ctgaccctcg agatcgaacc gctcgagggt
     2581 ggtgcgaagg actaccgcga gcagcgtggc ggccgtcgga aatccgcttc cgatagctag
     2641 cagatcgctc gcaaattagt ccttgttccc tcgcgaaatt gcgccctatg gtgcggcgcg
     2701 aagcacaagg gggaaacttg agcgcacaac cttcattcga atcctggcag caccgggggg
     2761 cctgtcgggg gccgcacgcc accgtgttct ttccgccgcc gcagttcgaa cgcaagcacg
     2821 agcgggcaga gcgcgaactt cgcgccaagg agatctgctc cgggtgctgc gtggcgaccg
     2881 attgcctcga ctacgcactc gagatccgtg agccccacgg catctggggc ggactgaacg
     2941 agaacgagcg tcgggtcatc ctcgaagctc gctccgctcg cgtctcctga cctccgtcga
     3001 gctgagaacg cgccgcggcg gcgcggcgag cggtccgaca tcgagcagta ccatttgccc
     3061 gtgttcgtcg tgatcctggg ccttctcctc atgggagtca tcgagattgc ggtgatggtg
     3121 gccgtctcga atgccctctc gtgggaggtc gcgctgctcg ccatcgtgct ggccggcgcc
     3181 gtcggggcgt gggtcgtcaa gcgggagggc acggcaacgt ggcgtcgcgt catgtccggt
     3241 gtgcgcgccg gccagatgcc gacgtcgtcg gtgctggacg gttgcctcgt tgtcgtcgcc
     3301 ggcgtcctgc tgctggtgcc cgggttcgtc accgacgtcg cggccatggc gctcgcgctg
     3361 ccaccggtac gacggctcgt gcgggtccgt gcaaccgacc acttccagcg tcgcatcgcc
     3421 gcccaggtca gccgcgcccg gaccacaacg accgtgttcg ggttcggcga ccccatgagc
     3481 tacggcgggt tcggcggccc cgggttcggc ccgggcggca cccggcgctc gaatcgcgac
     3541 gacgacatca tcgacctcga tgccgaggag gtgttcctcg acgagacgat cgccgagatc
     3601 gagccgccgc gggagtggcc tgcgtgaaga tgcacctcga tgtcgatcga cccgaggagg
     3661 tgccgatccg cgacgccgcc acggtgatgc tcgttcgcga cggcgtggac ggtgtcgagg
     3721 tgttcatgct ccggcgcaac ctcaactccg acttcgtcgg tggtgcctac gtcttcccgg
     3781 gtggcggggt ggacgccggt gacggtgctg cggaggtggg cgacgtgtgc gtcggccgtg
     3841 ccgacgccga cgcgtccgcc ctcctcggca tcgagtccgg cggcctcgca tactgggtgg
     3901 cggcgatccg ggagtcgttc gaggaggccg gcctgctgtt ggcggtcgac gacaacgatc
     3961 agatggtccg tttcgacgac ccgacggtca acgagcgctt cgtcgaacac cgtcgagcgg
     4021 tcgacgccgg cgaggtgtcg ctggtcgaca tcgcccgatc cgaggggctc cggctcgcca
     4081 ccgaccggat ccactacttc agtcgttggg tcacgcctct gggtgcgccc cgccgctacg
     4141 acacccgctt cttcatcgcg cacccgcccg agtggcagaa cccggtgcac gacgaccgtg
     4201 aagtgatcgc caacctgtgg atccgtccgg cggaggcgct cgaccgtcac gcccgcggcg
     4261 agttcgaact gatcttcccc acggtgcgga gcctcgaggc gctcggtcgg ttcgacacgg
     4321 cggagcagat cgtccggcac gcgtcggaga tcgcccacat cgagccgatc ctgccaacga
     4381 tcgtcgacgg tgaccacggg ctccgcatcg tgctgcccgg cgacggttcg tacgacgcgt
     4441 tgacgtccaa acgcctcgac tgacgggcgg ctggacggcc gacggtgtca gccgccggcg
     4501 acggggctgg cgaccttcgt gtcgtcgtcg gagtcgttcg ctccactgtc gtgcgcgtcg
     4561 agcgcagcat tgacggcgtc gagcagtcgg accgagcagc cggccaggtc gaccgggggc
     4621 gcgatgtcgg tcgacaacag gtgggcgagt tcgcgaaacg cctcggcggc agcgccgtca
     4681 ccgagggcga tcggtgtgcc ggtgtcgccg ccttcggcga cggcgttctc gatcgggata
     4741 cgcgccaaca gcggcgcacc gatcgtcttg gccagccgct gaccgccgcc ggtaccgaag
     4801 atcgcgaagc gctctccgtc gggcgtgacg aactcgctca tgttctcgac gacgccgatg
     4861 atgcgcaggt agttctgacg tcccatgttg acggcgcgta cggccacctt ctgcgcgctg
     4921 agggacggcg tcgtgacgac gatcatctcg gcccggggca ggagcttggc gagccccatc
     4981 tgcacgtcgc cggtgcccgg gggcatgtcg atcaggaggt agtcgaggtc atggccccag
     5041 tcgacgtcct cgaggaagtg ccggacggcc cgctggagga tgaggccgcg ccacatgagc
     5101 gcggtttcct cgtcgtcgac gagaaagccc atcgagacga tgcgcagctc accacgacca
     5161 acggtctgac ggtgcggcgc gatgcggccg tcggcctcgc ctgcctcgag gcggccatcg
     5221 acgccgagca ttcgtgggac ggagaagccc cagatgtcgg catcgatcac gccgaccttg
     5281 aaaccggtcg cggcgagcgc cgtggccagg ttgacggtga ccgacgactt gccgacgccg
     5341 ccctttcccg atgcgatcat cagcaccttg gtggtggcgg ggatggcggt cacaggagcg
     5401 cgctcggcga tgtttcggcg ggcggtggcc atggtggtgg ccttttcctc ggccgtgagc
     5461 tcggtccagt cgatcttcac cttcgtgacg ccggggaggg acccgacacg gtcgcggacg
     5521 tcgcgttgga tctgtgcccg cagcgggcac ccggccgtcg tgagcgccac ggtgatgcga
     5581 acgaagccgt cgggggacac cgttgcgccc ttcgccatgc cgagcgtcac gatgtcggag
     5641 ccgagctcgg ggtcgatgac accgcgcagc agcccgatga cgtcctcgct cgtggggagg
     5701 gtcggttcgc tcacgtcgcc attctacggc ggccgggcgc cgaggccccg gcgggcatgt
     5761 gccacgggtg gtggtggaat agggcctcgg ggcacgttgt tgcacggggc gctctgggtg
     5821 gatcgccgtt cgggcggtac actcacggtt gcgtcacgtc atcaggaggc ccagcacatg
     5881 atcactctga ccgattcagc ggcagtcaag gttcgtcagc tcatcgagtc ggaaggcgag
     5941 gagaacctcg cccttcgtgt cgccgttcgc cccggtggct gctcgggctt ctcctacgag
     6001 atgttcttcg acaccgacat cgccgccgac gacctgtcga gcgagttcga cggcgtaaag
     6061 gtggtcgtcg atccgtcgag cgcccagctc ctcaccggcg ccgagctcga ctacaaggac
     6121 agcctcgaga agtcggggtt cgagatcaac aaccccaacg cgcagaagac ctgcggttgc
     6181 ggctcgtcct tcagctgatt cgctgacgaa acctctccgt gcaattccag ctcaacggcg
     6241 accaggtcga ggtcgctgac gacggctgct cgctgctcga cgtccttcgg gacggtttcg
     6301 ggctgacgtc ggtcaaggac ggctgcagcc cccagggcca gtgcggctgc tgcacggtgc
     6361 tcgtcgacgg ggagccgcgg gtcagttgtg tcacgccggc gcgtcggatc gccgatcgtc
     6421 gcgtcgacac cctcgagggc ctcgcgcccg aggtcgcgct ggcctggggc gaggcgttct
     6481 gcgcgacggg cgggagccag tgcgggttct gcactcccgg catcgtgatg cgtctcgaag
     6541 ggctgcggcg caagggcggc gacctcggcg agcggccggc cgtcgaccga gcgctcgcgg
     6601 cccacctgtg tcggtgcacc ggctggcaga cgatcgtcga ggcggccgct gcctacggga
     6661 cgccgacgag cgacgaccgc gatctcgcgg ctgcgggacg ccgggcgtcg atcgagggtg
     6721 gcgaaccaca gcgagtcgcc cccgagatcg ccctcggacg cggtggcttc gccgatgacg
     6781 gggctcccga cgatgcggtg atcggcgtcg tcgatgccac gtccggttgg caggtcgccc
     6841 ccacgatggc ggaggcacgc cgtcaggtcg gcaaggtgca ggggcgccgc agcaccatcg
     6901 acgcgaccat gccggtcgac ctgcccgacg gagactgggc ggtcgagttg cagaccggct
     6961 gggtcgatcc cgcctacctc gagaccgacg cgtcgtggtg cgcgccgggc ggggagccgg
     7021 cgaatccgct cgccaacggt ggtgcgttcg gggcgaagca cacctcgccc gtgccggggg
     7081 acgcgcgccg tctcgccgac gagctcggca cccccgtgcg cctgcggtgg tcccgggagg
     7141 acgtcgtccg caacggcccg aagcgtccgc cggtcgccat cggtctgcgc tccgacggtg
     7201 gtggcgttgt ccgggtcgct cggacccccg gtctcgtcga tcggatcgcg gcgttcgcgc
     7261 cggagctgac ggtcgaggag gtcgacgtgg tcggtcccgc tacctccgcc gacgcccgcg
     7321 ccgccgggtg ggccgaggtc gccgtcgcga tggcggccct cgacgcggtg ccgggcacgc
     7381 cggtcgagat cacgtcaccg tcgggtggtt gggcatcggt gaccgtcgac gagcagggcg
     7441 cccacgtccg cgtgcgctgc ggtgcagtcc tcgacgcagt ggtgctgcgg agctactgca
     7501 tcggtgccgt ccacatggcg ctcgggtggg tgacgtcgga aggcctcacc gtcgacgccg
     7561 acggttcggt cgccagtctg accgttcgct cgttcggcat cgtccgtccc ggcgagatga
     7621 tccccgtcga ggtcgagatc atcgacgaac ccggggtcga gccggtcaac ggcagcgacg
     7681 ccgtcttcgc ggcgacggcg gcggccgtat ggatcgccca gggcactcct ccggcgtggc
     7741 cgaccggtcg cccggtcacc tccgtctgac cgaccggcgg gcgtaggatg ccggccatgg
     7801 ctccacctgt cggtccctac accccgctcg tccgtgccgg cgacttcctg atcgcctccg
     7861 gtcaactcgg cctcggcgac gccggcctcg tcgacggcgg tgtcgccggc cagctccgcc
     7921 aggcgatcgc caacctcgcg gcgctgctcg agtcggccgg ggcgtcgttg tccgacgtgg
     7981 tgaagacgac ggtgttcctc gtcgacatgg acgactacgc ggtcatgaac gacgtctact
     8041 gcgacgggtt cggcgatcat cgcccggctc gctcggccgt ggcggtggcg gccctgccac
     8101 tcggcgccgt cgtcgagatc gaggcctgga gccacgtccc ggcctgaagt cggtccgctt
     8161 gtggggcgag gcggggtggt gcggtaacct gcccccatgc tcggagccat cctgatcatc
     8221 atcgccctct tggtcgtcgt tcccgtcggc gtcctgatct cgggtggcgc gctcgccggc
     8281 atcatcggct tcttcgtcca gaaggacgtc gaccagctca acgagggttc cgagctcatc
     8341 gacctcaacg gctgagccac ccgctcgtct tcgagcactg gagcagtcga cgccggtcct
     8401 cccgctcccg cggctcgtgc gggtcggcca gaacggagtt cccatgcccg aacgctcact
     8461 cggcgacgtc gcccatgatc gctacggcct cgtcaccgac cgcgaactcg acgagctcgg
     8521 gatcaccgag ggcgaacgtc gtcgtcgcat cgccgccggt gagttcgagc gcctgaacgg
     8581 caaggtgctc gcggtcgcgg ggtcaccccg aacgtggtcc caacgcgctg cggccgcatt
     8641 gctggcggtg ccgagcggtg tgctcgggta cgggtcggcg gcgcgggtgc tcggcctgcc
     8701 cgagtaccgc gatcacgacg aggtcacgct gagcgtgccc ctggcggcgc actacttcat
     8761 tccgggcatc gagatccggc gcacgggtcg ctggtccgac gagcacgtga tggtcaccga
     8821 cgggctccgg cacaccacgc tcggccggac gctcgtcgac ctcgcgctcg tcacccctga
     8881 tcgtcgcctg caggacgtca tcgaggaagc ggtctcctca cagcgcatca cctgggacga
     8941 actcgaggcg acctaccgct cgctcgcagg tcagggccgc tcgggcaccg ccggcgcgcg
     9001 gcgggtcctc gaggccgtcg agggccggcc accctcggag gcggaactcg agcggatgta
     9061 caccgaactg ctgcagcgcg ccggcgtgcc ggtccccacg atgcggatga ccccaccgtg
     9121 ggcggaacgg caacccggac gggtcgacgc gatgtacgag gacgccacgg cgatcgtcga
     9181 gctggacggt cgcaggttcc gggtccgtca cgccgcgctc acggaggacg cacgacgtga
     9241 ccgcctcgcg agggagcacg gctacgacac ggtgaggttc acccacctgc agctccgcaa
     9301 cgaccccgac cacgtggtcg aggtgagtcg gaccctcagc gctcgaacct gaggcgaggt
     9361 ccgatcctcg gggtcagccc caacgggact cgccgaagcg gtagcccacc gagcgcacgg
     9421 tcgagatcat ctgcgcgtgt tgctcgccga gcttggcccg gagacggcgg atgtggacat
     9481 cgacagtgcg cgcgccgccg tagtactcgt agccccacac ccggctgagg agcgtctccc
     9541 gggtgaacac ccgcccgggg ttctgcgcca ggaacttgag cagctcgtat tccatgtacg
     9601 tcaggtcgag cgggtcgtcg tcgatcgatg cctggtaggt ctcgaggttg aggacgagcg
     9661 ccttgtagac gatcgtctcg gaatcgtcgg cgagcacacc ctggcgcgtg agcaggtgct
     9721 tgaggcgtgc ctcgaactcc atcgggtgga acggcgtgac gcagaagtcc tcgaacagct
     9781 cgtgacggct ctccagctcg ccgagctggg aaccgcgcac caacaggagg agcggcgcga
     9841 tgccgaggtc gccgcggccc agcatccggg cgaacgtgaa cgcaccgtcg gcatcgtcgg
     9901 acgcgtcgac gatcgccccg gcccagcttc cggccaccac cgacgcctcg tcggtcgcgg
     9961 tcgcccagga gtacccggcc atgtcgaggg aacgcacgac atcgtgtggg ggaggatccg
    10021 gccaacagag cagtggttcc attcgaaacg actccctacc agttcttcag atagcgatcg
    10081 agttcgaact ggctcacatg cgccttgtag tcgctccatt ccgcacgttt gttgcggagg
    10141 aaccactcgt agatgtgttc gcccagcgcc tcgcggacga ggtcggaggc ctccatcacg
    10201 tcgagcgcat cggagagcga ggatggcaac aatgggatgt ctgccttgac ccgttcggcc
    10261 tcggtcagct cgaacaggtt ggcgttggcc tcgggcggaa gttcgtagag gccgtcgacg
    10321 ccggccatgc cggcggcgag cagcaccgaa taggcgaggt agggattgca cgcgggatcg
    10381 agcgcccggt attcgatacg ggtgccagcc gcttccttac cccgtttggt cacggggatt
    10441 cgaacgagtg cggatcggtt gttgcgggcc cacgagacgt gcacgggagc ctcgaagccg
    10501 ctcaccaggc gcttgtagct gttgaccagc tggttggtca cggcggtgat ctcgggggcg
    10561 tggcggagca ggccggcgat gaactgctgt gcgacgggcg acaggttcga ctcggcgcct
    10621 tcctcgtaga aggcgttggt ctcgccggcg aagagggaga tgtgggtgtg catgcccgag
    10681 ccctgcaccc cggcgatcgg cttcggcatg aacgtggcgt gcacaccgtg ctggagtgcg
    10741 acctcccgca cgaccaggcg cagcgtcatc acgttgtcgg ccatcgacag cgcatcggtg
    10801 taccgcaggt cgatctcgtg ctgggagggg gcatcctcgt ggaacgagta ctcgacgggg
    10861 atgccggtcg cctcgagccg ctggatggtg gcgcgtcgga tgtccgacga cacgtccttg
    10921 gtggtgaggt cgaaatagct ggcggtgtcg agcgggaccg gtgcctgcga cgggtcgtcg
    10981 ctctcgaggt agaagaactc gacctcgggt gcgcacatca gcgtgaaacc ggcgtcgtgg
    11041 gcccgctgga gattgcgctt gagcacctgg cgggggtcgc cctcgaaggg ggagccgtcg
    11101 aggttgacga gatcgcagaa catgcgggcg ctcacctcgg cgccgtcgcc ccaggtcatg
    11161 agctggaagc tcgacgcatc ggggatcgcg agcacgtcgc tctcctgcac gcggctgaac
    11221 ccatcgatgg cggacccgtc gaactggatg ccgtcctcga acaccgactc gagttcgacg
    11281 ggcgagatcg cgaccgactt cagttgcccc aggacgtcgc agaaccagag gcggatcagg
    11341 cgaaccttcc gctcctcgac ggtgcggagc acgtaggact gctggtgttc catgccccta
    11401 ccttctacga actcgacggt tccgcaaaat tctcgggtcc cagttaacac agcgttcaca
    11461 ttggggcaac gggtgggaaa tcgcgtaggt tcagccttgc tccgaatcaa aaaacaggcc
    11521 gtccgctggc ggtctccgac aagaggtggt gtccgtgccg tacagggctg aatacatctg
    11581 gatcgatgga accgaaccga ctccgttgat gcgctcgaag accaagatca tcgagaatgg
    11641 caaggagccc ggcatctggg gcttcgacgg ttcgtccacg aaccaggccc ccggctccga
    11701 ctcggactgc gtgctcaacc cggtcttcca gtgccccgac cccacccgcg gtgagggcga
    11761 catcctcgtg ctctgcgagg tgctccttcc cggcacgctc gagccgcacc ccaccaacca
    11821 ccgtcgcgcc tgcctcgagg cgcacgagaa gtacgcgaag gacttcgagc cgatgttcgg
    11881 catcgagcag gagtacaccc tcttccagga gggccgtccc ctcggctggc ccaaggaggg
    11941 ctactactac cccgcaccgc agggccccta ctactgcggc gtcggctcgg tggagatcgc
    12001 cggtcgtgac ctcgtcgagg cccacacgac cgcctgcatc caggccggta tcggcatcgt
    12061 cggcaccaac gccgaggtga tgctcggcca gtgggagtac cagatcggca tcctcaacac
    12121 gatcgacgtc gccgaccact tgtggatgtc ccggtacctg ctgtaccgca ttggcgagga
    12181 cttcggcgtg aacgcgtcga tctcggcgaa gccgatcaag ggcgactgga acggcgccgg
    12241 ctgtcacacc aacttctcca ccaagcagat gcgcgagggt tgggatccga tcatcgccgc
    12301 gtgcgaggcc ctcggtggcc cgggcaaggt cgaggagcac ctcgccggct acggccacgg
    12361 ctacgaggag cgtctcaccg gcctgcacga gacggcgcac ttcagcgagt accgctacgg
    12421 cgtgtccgac cgtggcgcct cggtccgcat cccgtggcag gtgggtgtcg acaagaaggg
    12481 ctacatcgag gatcgtcgcc cgaacgccaa catcgatccc tacatcgtgt cgcgtctgat
    12541 cacgaacacc gtgtgcgagg cggcctcggc ctagccgagc gccacacgtc ggcccggtcc
    12601 gaccggtact gcgaacgagg ggccttcggg cccctcgttc cgcgtccagg tccgtgtgcg
    12661 gtcgtcctca cggcctgtgc caggcttggg cccgtgagtt ccgtgcgtgt cgccctggcc
    12721 cagctgaatc cgaccgtcgg cgatctcgtt tcgaacacgg acgcgatcct cgaggccctc
    12781 tccgagtccg aggcaggcgg tgcgaggatc actgtgttcg gtgagctcgc ggtgtgcggc
    12841 tacccgccgg aggacctcgt cctcaagcgg aggttcgtcg acgactgctg gactcaggtc
    12901 gaacggatcg ccgaggcgtc cgggtcgtgc gccgccgtgg tcggtgtgcc ccaacgggcc
    12961 gaggacggcc ggctctacaa cgccgcagtg gtgtgccgcg acggtcgggt cgactacgtg
    13021 tatcgcaagc aggagctgcc gacgtacgcc gtgttcgacg agcaccggtg gttccgtccg
    13081 ggtgacgtcg accagccgct gtgggacttc gacggtgttc cggtcggcat ctcggtctgc
    13141 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcgaccag ccgctgtggg
    13261 acttcgacgg tgttccggtc ggcatctcgg tctgcgagga catctggatt cccgatggcc
    13321 cggtgctgcg ccaggccacc gcgggggcgc gcctcctcct caacctcaac ggttcaccct
    13381 tccaccacga caagatcgtg caccgcgaga agatgctccg cgaccgggcg gtcgccgttg
    13441 cggcgcccat cgtgtacgtc aaccaggtcg gcggtcagga cgagctggtc ttcgacggcg
    13501 ggtcgttcgt catcggcggc gacggtgcgc tcgtggcgcg gctcccgcag ttccgcacac
    13561 acctcgaatt cgtggacgtc gaactcgacg gagcgtcggc gcccggtgac ctcgtcgttc
    13621 cccgattgga gagcgccgag gagatgtggt cggccctggt gctcggtacc cgtgactaca
    13681 cgatcaagaa cgggttcacc gatgtcgtga tcgggttgtc gggcggtatc gattcgtcga
    13741 tcgtggcggc catcgccgtc gacgcgctcg gtgcggaccg ggtccacggc gtgatgatgc
    13801 cctcgcggta cagctcggac ggctcgatca ccgacgccga acgtctttgc aacacgctgt
    13861 ccatcgaatc gcgcgtcatt cccatcgagg ccgggcaccg tgcgttcgag gagatgttgg
    13921 caccctcctt cgaggggatc gcacccgacc tcaccgagga gaacctccag tcgcggctcc
    13981 ggggcgtcac gctcatggcc ctctccaaca agttcggctg gttggtcctc accactggca
    14041 acaagagcga gtcggcggtc ggctactcga cgctctacgg agacaccgcc ggcgggctgg
    14101 cggtgatcaa ggacgtctac aagaccgtgg tctacgacct ctgtcgctgg cgcaacgacg
    14161 aggcggggca cgagatcatt cccgaatcgg tgctcaccaa ggcgccgtcc gccgagttgc
    14221 gacccgatca gcgcgacgac cagagcctcc ccgcctacga ggtgctcgat ccgatcctcc
    14281 gcggctacgt cgacggcgac cagaccgtga ccgacctgat cgaggcgggt cacgacccgg
    14341 cggtcgtcgg gcgcatctgc cgcctcgtcg acatcgccga gtacaagcgc cggcagaacc
    14401 cgccgggcat ccgggtgacg tcgaaggcgt tcggtcgcga ccgccggttg ccgatcacga
    14461 accggtaccg ataacccaac atctcgacgc cccccatcgc accgggcccg aaccccccat
    14521 ctggatcccc gggacgactg ctgggcgttg gtaggttccg tgcggtgttg aggtcccttg
    14581 cgttggcgtt ctgcgacgtg ggcgcgggcg tgctggccct cgccggcctc atcaagttct
    14641 tcgacccgac cccgtgggtg acctccctcg gagcgctcgg cgtgcccctc ggcccggtcc
    14701 gttttcgtcg atcggtggtc gccgcggcgc ggctggtcgg cgtgagcgag gtcgcgatcg
    14761 ccgtcctcgt gttcgccgtc ggcgggaccc ttgcccttgg tctgctggcg gtcgcgtacg
    14821 ccgtgttcgt tgtcgtgtcc gtggttgcca ctcgccgggg cggcgcctcg tgcgggtgct
    14881 tcggcgtccg gtcggcaccg atctcgtcgt tgcacgtggg cctcgacgcc gtggtcgcgg
    14941 cggcggccgt cgtcggtgcg gtcgacgggg ccgacgcact cgccgtgcgg ttgggggacg
    15001 gggtcggtgc gaccgtcgcg tacctcgtgt tcctcggcct cggggtggcg gcgatggtca
    15061 cggtgatgac gacgggggcg gagttgtggg cgctcaagcg
//
LOCUS       DAOSOA010000034        14999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4006_length_14999_cov_7.236684, whole genome shotgun sequence.
ACCESSION   DAOSOA010000034 DAOSOA010000000
VERSION     DAOSOA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4006_length_14999_cov_7.236684"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PKY13_03935"
     CDS             complement(<1..1390)
                     /locus_tag="PKY13_03935"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOT79109.1"
                     /translation="MTMRRALVPLLGLLALVATACPVSTPGGGGTTTTTIDPGPPVAV
                     AGASPSIGDAPLTVTFDSSGSTAGSGTGLTFAWDFGDGSPVDNSIAPTHIYNTPGSYN
                     AKLTLTNSAGTSTSPNIPIQVNVDPNPKYYVRTTGGTGSTCGPLANPCSTITQAVTNA
                     SANGIHAIRVAGGSFTGTVNVPSNLEIVGGYKQNFTDTDPAEVTTIFGTATSPAAVFS
                     GVTGSKISAVSLQGVTRTSGDAVGVLITGGSSGVSVGDAANAQTIVSGGTGPNATGIL
                     VTGGSLASVVYAKVNSGSTVGAGKSAYGVRALGLSVVNVTLSDVTAQPGVAGTSAPGT
                     VPGQATSGGTGGRGQDSGGSGGGGGGGTTHAGGSGGGGGGFYAAGGNGNGGGGPAGGG
                     GGAGGCGSAFGCGDEADGGGAGGSGAAGSAGAAGNNNPAVVAGDLYTPTNGTAGTAGS
                     AGSGGGGGGGGKS"
     gene            complement(1552..3522)
                     /locus_tag="PKY13_03940"
     CDS             complement(1552..3522)
                     /locus_tag="PKY13_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79110.1"
                     /translation="MAFALVAGMATSCAPDSSSGGGTNTTQQFCEFWDKVESAPPTEN
                     NAVLVKDDVVALADAATVTGASCTASNAKVALSGATLAEGEEVPSEQGNTTSDPVAAV
                     TGDTIAAGEPVLDNLSVQTLSAEIGLNGITVRGNVAVRLSGVTSTIGFVGTLANLDNW
                     SINLSSAGLTIPGITTSPVTFNGTLAVVNGVPSLSMTALASSVKIGDVSVTGATLKLN
                     ASPVTGVSATVAGSLKIGPSTASGTVNVAFDKAGALVSAKAQIDAHLVGSMAGGKKVD
                     LIGSVKLDGNGKETVASFKASGVVGDLNVNEANGSLTLQPNKATFVGKLDVNQGGNFV
                     RFDGTIVWDGITAYTPFLQLEGGGDISGTLDDGTTFAASGTLSVISVGGQVRTELNGD
                     FKLGALKLHGSAGVTIAGPTTSLDINADLVGAGFSASLSGAVTITDGRAELISLDAVV
                     NGSIQMGDLTLTGASLKIRSTYGSPLDVSFNGGLQVGSKASVTGALKATFGPNGTLLS
                     LVGDVYGTLGLDGWTVNFNGQVLASSEQVTLSGSGAVLASNFPLGITINGSFTSKLTN
                     PSWSLNGYGRVRLASIDVASARVSLSQGVGMKATRMGFYFNIIGINFYFEGDFYLTPA
                     GGCDHVDITGGSFLMKPALKLVLPGVINCPVY"
     gene            complement(3720..5096)
                     /locus_tag="PKY13_03945"
     CDS             complement(3720..5096)
                     /locus_tag="PKY13_03945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOT79111.1"
                     /translation="MSILTSRETTTPGTGADPVGTDALVRLVTAVSRSYGHFTLLLDD
                     EFRVIWGSETLSRIFGWTDVVGRNVVDLVHPEDLELVLAGIDYHSEHAAEYQRYEGTW
                     RPDVTTMRVAHANGGWVRCEVSVFNHLGDPVIGALLASGRLADDRSDMALAIDLLGSG
                     AVLGEVLPVVARLVDRTIDGVRAQVMWWDDTSDWVISAPDAAELPSPPADLVAAVLRT
                     GEMEVSTGIELVSHPGIDTDFAAVWVLPIGAPGRDLVVGCLVVWCRLPLSLVTGPQQP
                     IHQAVRLASLAIVENHAKSALRWEASHDPLTALRNRAGFNTEVSASSGSCALLYIDLD
                     DFKGINDRLGHQAGDAVLVEVARRIRAVVRDQDTVGRLGGDEFAVLCPDLADADVADD
                     IARRLVESVSRPVSVGGQPVTVGASVGIAFGSHPSERSSLIRRADEALYQAKAGGKGR
                     VIVAPDPI"
     gene            complement(5187..5648)
                     /locus_tag="PKY13_03950"
     CDS             complement(5187..5648)
                     /locus_tag="PKY13_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT79112.1"
                     /translation="MPLIPVDPTDRALATELLARYAEAVDAGDFDAVGALLADATIED
                     AVGNEITSGAVGVAALFAATTRRHEDGTPLTGHVITNVIVEAVGSDELELRSRFTVFQ
                     AAPGLPLQPIVIGRYVDRVVRGADGWRFARRRMIPERWGDVSRHLTFDPTA"
     gene            5827..6315
                     /locus_tag="PKY13_03955"
     CDS             5827..6315
                     /locus_tag="PKY13_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79113.1"
                     /translation="MWAQPDGRRVLLAPDQRTADFVTAVYGFDDVRVVSFEEPSGREP
                     NELHVVAGPVALRFVGGRPWLLLPPRPRWFTRRVEAPIAAALLGVHTWGVSPTGVQEW
                     YQARSMRFVRTATASIDGRDLGARRPLDPACGFGFSEAPRWPTIVAVRPTLERPPGAP
                     AW"
     gene            complement(6323..6955)
                     /locus_tag="PKY13_03960"
                     /pseudo
     CDS             complement(6323..6955)
                     /locus_tag="PKY13_03960"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
     gene            7067..7507
                     /locus_tag="PKY13_03965"
     CDS             7067..7507
                     /locus_tag="PKY13_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79114.1"
                     /translation="MRSASDPDTEPTIERTVLLDADVDRVLEALSSPDLLAAWLGEWT
                     QTDDHSASVITDDGVRRNVERVAADRADEVRWRWSPADDPSQWSDVRFTVTTEGQRIR
                     LTVVETAATRTVPASASATAPAVSWLGSLMALGAVLAVGSLVAV"
     gene            7513..7845
                     /locus_tag="PKY13_03970"
     CDS             7513..7845
                     /locus_tag="PKY13_03970"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT79115.1"
                     /translation="MPDPAGTDAVFAALADGTRRELLRAIVAQGPCTATELASDRDIT
                     RQAISKHLAVLGQAGLVSGHRDGREVRYQADPRPLDVATEWITDTGAAWDRRLGRLQD
                     LLGRRPSK"
     gene            7874..8392
                     /locus_tag="PKY13_03975"
     CDS             7874..8392
                     /locus_tag="PKY13_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79116.1"
                     /translation="MAITRLNHAVLYVRDVAVTREFYENVLGFETLMWMPGRAAFFRA
                     DGSTNDHDLGTFQIGSDAGPTTAGRTSVGLYHLAWEVDTLAELARLRDVLAERGSLVG
                     ATDHITTKALYAHDPDGLEFELSWLVPLDRIDDELRAEASEVMGRPLDLAKEIERYGA
                     ETLGGIGVSIPA"
     gene            complement(8344..9624)
                     /locus_tag="PKY13_03980"
     CDS             complement(8344..9624)
                     /locus_tag="PKY13_03980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT79117.1"
                     /translation="MTRLRLLSSETFSSLSIRNFRLFFAGQSISQVGNWLTLVAQALL
                     VLKITGNGVAVGLLTAFQFAPVLLLGPWAGLVADRSDKRKLLMRVQMFAMAQSFALGA
                     LAFMDRPPVGAIYAIAFFGGVATAFDNPARRSFVVEMVDGEHVNNAVSLNSALMTGSR
                     VVGPALAGLLISTVGYGWCFIVDAVSYVAVIIGYRMMDPTKIHAGPVTPRGKGQVRAG
                     LRYAMGKDELRIPLLMMAIIGTLAFNFTVVMPLLIKDVFHGSDATFTIFFSVISLGSL
                     FGALYTARRRAVSIHDVVVSAAVFGVAMLVLAIAPNLWFAFPVGVVFGAASIGFMTAS
                     TSIVQVEADPAMRGRVLALQAMVFLGSTPIGGPIVGLVCDWFGARAGVALGGVATVAA
                     AGYGFWALRHLPGRGGVTTYAGIDTPMPPRVSAP"
     gene            complement(9621..10130)
                     /locus_tag="PKY13_03985"
     CDS             complement(9621..10130)
                     /locus_tag="PKY13_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT79118.1"
                     /translation="MPRTTPTISNPGASESNAPDIDEVAASLRMSIARLARLLRQQDQ
                     SGMSPALNTAMVSVWKHGPLTLGHLAQHEQVTPPTVSKLVDKLEQRGFIERISDPADR
                     RVTRVAITPAGIEQIEEIRGRRTEWLSARLRELPADDVARLHDVTLVLEHLVTPPQPP
                     TTNHERTNP"
     gene            10270..10767
                     /locus_tag="PKY13_03990"
     CDS             10270..10767
                     /locus_tag="PKY13_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT79119.1"
                     /translation="MTTSPSSNFPADEVEAALATYVELRERIGRREADWTDLGDLFTD
                     DAVYIDPAWGRLQGNDELRRFFDESMRGLEDWDFPIEYTAITGDTVVIKWWQQLPGTR
                     PDGTRYQQSGISTLVYAGDGKFSYEEDLLNMTHVLEDLAASGWRPGKGFIAPPAQPDR
                     NFARP"
     gene            10782..11279
                     /locus_tag="PKY13_03995"
     CDS             10782..11279
                     /locus_tag="PKY13_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT79120.1"
                     /translation="MALDDELLNRVTWKIPNALALVGSAAGGRRNAMTTSWITQLAQE
                     PVLIGVGVDNDSVTVGLIRDGGSFSINLWDAENTKVFVKFSKPAVDEDGTLNGRAVTT
                     ATTGAPIFEEAIAWMDCEVRHTLDLGSHTLFVGEVVAAAIPNDEARPASMSDTRMKYG
                     GVRRH"
     gene            11333..12316
                     /locus_tag="PKY13_04000"
     CDS             11333..12316
                     /locus_tag="PKY13_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HOT79121.1"
                     /translation="MTDTTTTVGKLADATDRVPGSGLAAMRFRMEPFTVQVGPTTHLA
                     AESERLAGLTFEHFDVRRMGATIGAELHGIDLTDDLPQGAIDEIATALAEFKVIFFRD
                     QPLTAEQHVAFARRFGDLEIHPFIPANTGQPELVRLAKSAEIGGYENAWHHDVTWREA
                     PSMGAVLHAVQVPDVGGDTLFCDMYAAYEGLDAATKAEIDHLDAVHDYMRTFGHFIPD
                     DKKAETRAKYPQVTHPVVCTHPVTGRRHLYVNRNFVDHVAGVTPDESTALLDRLCRQA
                     DYPEYQCRFRWEDDSVAFWDNRAVQHYASSDYWPHVRVMERASIIGTRPVR"
     assembly_gap    12325..12424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12425..13002)
                     /locus_tag="PKY13_04005"
     CDS             complement(<12425..13002)
                     /locus_tag="PKY13_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HOT79122.1"
                     /translation="MTQTDETVIAPEGFAQQIRQATWVDHGKAEHAGYMDELTAGSLT
                     QYEYAEMIVQMWFVYGALEGAGERLAGDPTVAPFLDPALIRMPRIEADLEVLLGEDWR
                     ADATATPETEAYVARLGEVADWPGGFIAHHYTRYMGDLSGGQYIGRLVRRLYEVTSEA
                     GAAFYDFAGIDDVTAFKDAYRSHLDAVPFDEA"
     gene            13130..13441
                     /locus_tag="PKY13_04010"
     CDS             13130..13441
                     /locus_tag="PKY13_04010"
                     /inference="COORDINATES: protein motif:HMM:NF022083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HOT79123.1"
                     /translation="MTDAPHQFDEATIAGVTAHMNADHGADTLLICRSLGDRPDATAA
                     TMTGLDGDGGDYLVTLPDGDETIRIPWSRPLTERGEIRVEVVRMYEAACAQLGVEPRP
                     H"
     gene            complement(13533..14102)
                     /locus_tag="PKY13_04015"
     CDS             complement(13533..14102)
                     /locus_tag="PKY13_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79124.1"
                     /translation="MTTATTLRTDRPRRGLRAFVGATVLAVGLTGCFPDPGLPPTVGA
                     AITVSKTVGIQPGDVITVSGTGFTPTGNIGTRPPLAGAPAGVYVVFGRFAEVWKPSAG
                     AGGRTVLLQKWALPQSSIDTLVAFPWNQDPAEFVTIGADGSWTAQITVPAFDPAGGGY
                     GFAVYPGSGASNAGEELLERVAVAAPVPT"
     gene            complement(14205..>14999)
                     /locus_tag="PKY13_04020"
     CDS             complement(14205..>14999)
                     /locus_tag="PKY13_04020"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79125.1"
                     /translation="AAAALDWYAMAFEAVETVRYAGDDGRIGHAEMLIGGARLMLSDP
                     YPEMAVVPPDPSGSSVTLHLNLPDVDAVYARAVAGGAAGLREPEDQPYGERSSAVIDP
                     FGHRWMIQTTIAEPSVDEINQNLEGFTVVETPVAAPVATPPVTPVEADRPIELGYLTI
                     GFDDTAKARAFYGSLFGWRTETGNMGDPYAHIDNTELPMGMTPEGVDSAPVLYFRVPD
                     TSVFAAKVIELGGSVVSESDYESGGDVVCRDDQGREFHLWQPAPGY"
BASE COUNT         2387 a         5165 c         5172 g         2175 t
ORIGIN      
        1 cggacttgcc gccgccaccg ccgccgccac cgctgccggc cgaaccggcc gtgcccgccg
       61 tgccgttggt cggggtgtag agatcaccgg cgacgaccgc cgggttgttg ttgcccgctg
      121 cgcccgcaga cccggccgca ccggagccac cggcgccgcc gccgtcggcc tcgtcgccac
      181 agccgaatgc gctgccgcaa ccgccggcgc caccgccacc gccggccggg ccaccaccgc
      241 cgttgccgtt gccgcctgct gcgtagaagc caccgccgcc gccaccgctg ccgccggcgt
      301 gggtcgtgcc accgccgcca ccgccaccgg agccgccgct gtcctgaccc ctgccgccgg
      361 ttccaccgga ggtcgcctgg ccgggaacgg tgcccggggc gctggtaccg gcgacgcccg
      421 gctgcgccgt gacgtcggac agggtgacgt tgaccaccga gagcccgagg gcccgcacgc
      481 cgtaggcgct cttgccggcg ccgacggtgg agccgctgtt gaccttggcg tagaccacgc
      541 tggcgagcga gccgcccgtg acgaggatgc cggtcgcgtt cggcccggta ccgccactca
      601 cgatggtctg ggcgttcgct gcgtcgccca ccgacacacc actcgaaccg ccggtgatca
      661 gcacgccgac ggcgtcaccg ctcgtgcggg tcacgccctg gaggctcacg gcactgatct
      721 tcgagccggt gacaccactg aacactgcgg ccggcgacgt cgcggtaccg aagatcgtcg
      781 tgacctcggc cgggtcggtg tcggtgaagt tctgcttgta gccgccgacg atctcgaggt
      841 tcgaggggac gttcacggtg ccggtgaacg agccgccggc gacacggatg gcgtggatgc
      901 cgttggccga tgcgttggtg acggcctgcg tgatcgtcga gcacgggttg gcgagcgggc
      961 cgcaggtcga cccggtgccg ccggtggtgc gcacgtagta cttggggttg gggtcgacgt
     1021 tgacctggat cgggatgttg ggcgacgtcg aggtgccggc cgagttcgtg agcgtgagct
     1081 tggcgttgta cgagcccggc gtgttgtaga tgtgtgtcgg agcgatcgag ttgtcgaccg
     1141 gcgagccgtc gccgaagtcc caggcgaagg tcaacccggt tcccgatccg gccgtcgagc
     1201 ccgacgagtc gaacgtcacc gtgagcggtg cgtcgccgat cgacggcgag gcaccggcga
     1261 ccgcaacggg cgggcccggg tcgatcgtgg tggtcgtggt gccaccaccg cccggggtcg
     1321 aaaccggaca tgccgtggcg accagcgcca gcagaccgag caggggtacg agcgcgcggc
     1381 gcatcgtcat cgattttctc cagccatgtg tttggttcgc aaccatcgaa ccgtccctcg
     1441 aatgtgagct tatatcgctg tgactcatgc cacaacggtg tagatcgtcc cggcaagaac
     1501 tccccggacg agggaccggg cggtggctcg aggccaccgc ccggtccact gtcagtagac
     1561 ggggcagttg atgacgcccg ggagcaccag cttcagcgcc ggcttcatga ggaagctgcc
     1621 accggtgatg tcgacgtggt cacaaccgcc ggcgggcgtc aggtagaagt cgccctcgaa
     1681 gtagaagttg atgccgatga tgttgaagta gaagcccatg cgggtcgcct tcataccgac
     1741 gccctgcgac agggagaccc gggcgctggc aacgtcgatg gaggccaggc ggacccggcc
     1801 gtagccgttg agcgaccaac tcgggttcgt cagcttggac gtgaacgaac cgttgatggt
     1861 gataccgagc gggaagttcg acgccaggac ggcgccgctg cccgacagcg tgacctgctc
     1921 ggagctcgcc agtacctggc cgttgaagtt gacggtccag ccgtcgaggc cgagcgtgcc
     1981 gtacacatcg ccgacgaggc tgagcagcgt gccgttcggg ccgaacgtgg ccttcagggc
     2041 gccggtgacg ctggccttgg acccgacctg caagccgccg ttgaacgaga cgtcgagcgg
     2101 gctgccgtat gtgctccgaa tcttgaggct ggccccggtg agcgtgagat cgcccatctg
     2161 gatggagccg ttgacaacgg cgtcgagact gatcagctcg gcccgaccgt cggtgatcgt
     2221 gaccgcaccc gagagcgagg ccgagaagcc ggccccgacg agatcggcgt tgatgtcgag
     2281 gctggtcgtc ggcccggcaa tcgtcacgcc ggccgagccg tgcagcttga gcgcacccag
     2341 cttgaagtcg ccgttcagct cggtgcggac ctgaccgccg accgagatga cgctcaacgt
     2401 gccactggca gcgaacgtgg tgccgtcgtc gagcgtgccg gagatgtcgc cgccaccctc
     2461 gagctgcagg aacggcgtgt aggcggtgat gccgtcccac acgatcgtgc cgtcgaagcg
     2521 gacgaagttg ccgccctggt tgacgtcgag cttgccgacg aaggtggcct tgttgggctg
     2581 caacgtgagc gagccgttgg cctcgttgac gttgaggtca ccgacgacgc cgctggcctt
     2641 gaacgaggcg accgtctcct tgccgttgcc gtcgagcttg accgagccga tgaggtcgac
     2701 cttcttgcca ccggccatcg atccgacgag gtgggcgtcg atctgggcct tcgccgagac
     2761 gagcgcaccg gccttgtcga aggcgacgtt gacggtgccg ctggccgtgc tcgggccgat
     2821 cttcaggctg ccggcgaccg tggccgacac cccagtgacg ggtgatgcgt tgagcttgag
     2881 cgtggcgccg gtgacgctga cgtcgccgat cttcacggag ctggcgagcg cggtcatcga
     2941 caacgacgga acgccgttga cgaccgcgag cgtgccgttg aacgtgaccg gggaggtggt
     3001 gatgccggga atcgtcaggc cggccgacga caggttgatc gaccagttgt cgaggttggc
     3061 cagcgtgccc acgaagccga tggtcgacgt cacacccgag aggcggacgg cgacgttgcc
     3121 gcggaccgtg atgccgttga ggccgatctc ggcggagagc gtctggaccg acaggttgtc
     3181 gaggacgggc tcgcccgcag cgatggtgtc gccggtgacg gcggcgaccg ggtcggaggt
     3241 ggtgttgccc tgctcggagg ggacctcttc gccctcggcg agggtggctc cgctcagcgc
     3301 caccttggcg ttggacgccg tgcagctggc gccggtgacg gtcgctgcgt cggcgagggc
     3361 gaccacgtcg tccttgacca gcaccgcgtt gttctcggtc ggcggggcgg actcgacctt
     3421 gtcccagaac tcgcagaact gctgggtcgt gttcgtgccg ccgcccgacg agctgtcggg
     3481 tgcgcacgag gtcgccatgc cggcgaccag tgcgaatgcc aacgcgaggg ttgacagccg
     3541 acccgttgcg gtccgccgtt tggacgtgtg atgcatgtag cccccaccaa tttcggtcgc
     3601 gcggtgccac ccgcgagacg cagcctcagg tcagcataca catgaggatg tgccgagcgt
     3661 cacgtccaga tgcgacggat gggtcccgac gatcggcgct ggcgggccgg agccggtgat
     3721 cagatcgggt ccggagcgac gatcacgcgg cccttgccgc ccgccttggc ctggtacagc
     3781 gcctcgtcgg cgcgtcggat caggctcgac cgctccgacg ggtgggaccc gaatgcgatc
     3841 ccgacgctgg caccgaccgt gacgggttga ccccccacac tcaccggccg gctcaccgac
     3901 tcgaccagac gacgggcgat gtcatcagcg acgtcggcgt cggcgagatc ggggcacagc
     3961 accgcgaact cgtcgccgcc cagacgcccg acggtgtcct ggtcccgcac gacggcgcga
     4021 atgcgtcgcg caacctcgac gagcacggcg tcgcccgcct ggtggccgag gcggtcgttg
     4081 atgcccttga aatcgtcgag atcgatgtac aacagcgcgc acgaacccga cgacgcactg
     4141 acctcggtgt tgaacccggc acggttccgc agcgccgtca acggatcgtg gctcgcctcc
     4201 caacgcaacg ccgacttcgc atggttctcc acgatcgcga gggacgcgag ccgaaccgcc
     4261 tggtggatcg gctgttgagg gccggtgacg agcgagagcg ggagacggca ccagaccacg
     4321 aggcagccga cgacgaggtc gcggcccggc gctccgatcg ggaggaccca caccgcggcg
     4381 aagtcggtgt cgatgccggg gtgcgagacc agttcgatgc cggtcgacac ctccatctcg
     4441 ccggtgcgga ggaccgcggc gacgagatcg gccggagggc tcgggagctc ggcggcgtcg
     4501 ggcgccgaga tcacccagtc cgacgtatcg tcccaccaca tgacctgcgc ccggacgccg
     4561 tcgatggtgc ggtcgacgag gcgggcgacg accggcagga cctcgccgag cacggctccg
     4621 ctgccgagga ggtcgatcgc cagcgccatg tcactgcgat cgtcggcgag acgaccgctg
     4681 gcgagcagcg caccgatcac cggatcgccg aggtggttga acaccgacac ctcgcagcgg
     4741 acccacccac cgttggcgtg ggcgacacgc atcgtcgtca cgtcaggacg ccacgttccc
     4801 tcgtagcgct ggtactccgc agcgtgctcg gagtgatagt cgatgccggc gagcacgagc
     4861 tcgaggtcct cggggtgcac caggtcgacg acgttgcggc cgaccacgtc ggtccagccg
     4921 aagatacggg agagggtctc ggagccccag atcacccgga actcgtcgtc gaggagcaac
     4981 gtgaagtgcc cgtacgaacg cgacaccgcc gtcaccagcc gcacgagcgc gtcggtgccg
     5041 acaggatcgg cgccggttcc cggcgtcgtc gtctcgcggc tcgtgaggat gctcatctcg
     5101 ttcgatatcg accgcccgca ggaggcgctt gagatctcac gggcgtgacg tgtggcgggg
     5161 gcgggatcgg cgcccgccgg cgacgctcag gcggtcgggt cgaaggtgag gtgtcgggag
     5221 acgtctcccc accgctcggg gatcatccgg cgccgcgcga acctccagcc gtcggcgccc
     5281 cggacgaccc ggtcgacgta gcggccgatc acgatcggtt gcagcggaag tcccggtgcc
     5341 gcctggaaca cggtgaagcg tgatcgaagc tccagttcgt cggagccgac ggcctcgacg
     5401 atcacgttgg tgatcacgtg cccggtgagc ggcgtcccgt cctcgtgacg gcgcgtcgtc
     5461 gcggcgaaca gggccgcgac accgacggca ccggacgtga tctcgttgcc gacggcgtcc
     5521 tcgatggtgg catcggccag cagcgcgccg acggcatcga agtcgccggc gtcgacggcc
     5581 tcggcgtacc gggcaaggag ctccgtggcg agggcgcggt cggtcgggtc gacggggatc
     5641 agcggcatga acgtcatctc agcacgcgcg gtgccatgct cgcgtcctgt gtcgaggtca
     5701 ccgggatcga actcagcggt caggacccag ttggtcggca cgatctcctc gtgcgggttc
     5761 gagtccgggg accggttcgt cgtcgggcgt tgggaccgtt cgccggtcgg ccgcacgatc
     5821 gacgtcatgt gggcgcaacc cgacggccgg agggtcctgc tcgcccccga ccaacggacc
     5881 gccgacttcg tgacggccgt ctacggattc gatgacgtgc gggtcgtgtc gttcgaggag
     5941 ccgtcgggtc gcgaaccgaa cgagctgcac gtggtcgccg ggcccgtggc actgcggttc
     6001 gtgggcgggc ggccgtggct cctcctgccc ccgcgaccgc gctggttcac acgccgcgtc
     6061 gaggcaccga tcgccgccgc cctcctgggt gtgcacacct ggggtgtcag tccgacgggg
     6121 gtgcaggagt ggtaccaggc gcggtcgatg cggttcgtcc gcacggccac cgcgtcgatc
     6181 gacggccgcg acctcggtgc ccgccgaccg ctcgaccccg cgtgcggctt cggcttctcc
     6241 gaggcaccga gatggccgac gatcgtggcc gtgcggccga ccctcgaacg gccgccaggc
     6301 gccccggcct ggtgaatggc gctcagccga ctgcgacacc gtcggcctgg agcgcgccga
     6361 ccacctcgag cggccggcgg gacaggatgt ggtcgacggc gccgtactcg acggcctgct
     6421 ctccacggat gatgtagtcg cggtcgaggt cggcgacgat ccgttcgcgc tcctgcccgg
     6481 tcgccgccac gaggatgtcg accatgcgct cgcgcatctc gaccatctcg cgcacctgga
     6541 tctcgatgtc gaccgactgc ccctgggcgc cgccgtgggg ttgatggaac agcaccctgg
     6601 cgttcgggag gatgtagcgg tggccgggct cgccggcggc gagcaggacg gcggccgccg
     6661 aggcggcctg cccgacgcag atcgtcgcca ccggcgcctg gatgaactgc atcgtgtcga
     6721 cgatcgcgaa gaacccggtc atctccccac cggggctgtt gatgtagagg ttgatgcgct
     6781 ggctcgggtt ctcggcctcg agatgcaaga gctggccgac gaccaggttg gcgacctgtt
     6841 cgtcgatcgc cgagccgagg aacacgatcc ggtccgacaa cagacgggag tagaggtcgt
     6901 aggcccgttc tccctggggg ctctgcatca acacggtggg gatgatcacg cgggtgctcc
     6961 tttgtcggtc gggcctgcgt cagccgaacc ggtcacgtgc aaccatttgg ttgcttctcg
     7021 catggtaggg gtggtgatcc gaacacgcaa ccgctacgtt gcacatatgc gttccgccag
     7081 tgaccccgac accgaaccca ccatcgaacg gaccgtgctg ctcgacgccg acgtcgaccg
     7141 ggtcctcgaa gcgctgtcat cgcccgacct cctcgccgcc tggctcggcg agtggaccca
     7201 gaccgacgac cacagcgcat cggtgatcac cgacgacggc gtccgccgca acgtcgaacg
     7261 cgtcgcagcg gaccgggccg acgaggtcag gtggcgatgg agccccgccg acgacccgtc
     7321 gcagtggagc gacgtccggt tcacggtgac gaccgagggc caacgcatcc ggctcaccgt
     7381 cgtcgagacc gccgccaccc gaaccgtgcc tgcatcggcc tcggcgaccg cccccgccgt
     7441 ctcgtggctc ggcagcctca tggctctcgg cgcggtcctg gccgtcgggt cgctcgtcgc
     7501 ggtctgacgc cggtgcccga ccccgccggc accgacgccg tcttcgcggc gctcgccgac
     7561 ggcacccggc gcgaactcct gcgcgccatc gtggcccagg gtccctgtac cgccacggaa
     7621 ctcgcgtccg accgggacat cacccgccag gcgatctcga agcacctggc cgtcctcggc
     7681 caagcgggcc tcgtgtcggg ccatcgcgac ggacgcgagg tccgctacca ggccgacccg
     7741 cgcccgctcg acgtggcgac cgagtggatc accgacaccg gcgccgcgtg ggaccgccgg
     7801 ctcggccgcc tgcaggacct gctcggccgg cgcccgtcga aatagccgct cccccaccga
     7861 cgttgcaccg gccatggcca tcacccgact gaaccacgct gtcctctacg tccgggacgt
     7921 cgccgtcacc cgcgagttct acgagaacgt gctggggttc gagaccctga tgtggatgcc
     7981 gggccgagcc gcgttcttcc gtgccgacgg atccaccaac gaccacgacc tcggcacgtt
     8041 ccagatcggg tccgacgccg ggcccaccac cgccggacgg acatcggtcg gcctgtacca
     8101 cctggcgtgg gaagtcgaca cgctcgccga actggcccgg ctccgtgacg tcctggccga
     8161 acgcggctcg ctcgtcggcg ccaccgacca catcaccacc aaggccctct acgcccacga
     8221 ccccgacggc ctcgagttcg agctgagctg gctcgtcccc ctcgaccgca tcgacgacga
     8281 actccgcgcc gaggcatcag aggtgatggg acgcccgctc gacctggcga aggagatcga
     8341 gcgttacggc gccgagaccc tcggcggcat cggcgtgtcg atcccggcgt aggtcgtgac
     8401 gcccccgcgg ccgggcaggt ggcgcaacgc ccagaacccg tagccggcgg cggccaccgt
     8461 cgcgactccg ccgagcgcca caccggcccg agcgccgaac cagtcgcaca ccagccccac
     8521 gatcggcccg ccgatcggtg tgctcccgag gaacaccatc gcctgcagcg ccagcacacg
     8581 accccgcatc gccgggtcgg cctcgacctg cacgatcgac gtcgatgccg tcatgaagcc
     8641 gatgctggcg gcgccgaaca ccaccccgac ggggaaggcg aaccagaggt tcggggcgat
     8701 ggcgagcacc agcatcgcga cgccgaacac cgccgccgac acgaccacgt cgtggatcga
     8761 cacggcgcga cgccgcgccg tgtagagcgc accgaacaac gacccgagcg agatcaccga
     8821 gaagaagatg gtgaaggtcg cgtccgaacc gtggaacacg tccttgatga gcagcggcat
     8881 gacgaccgtg aagttgaagg ccaacgtgcc gatgatcgcc atcatcagca gcgggatgcg
     8941 caactcgtcc ttgcccatcg cataacggag accggcacgc acctgccctt ttccgcgtgg
     9001 cgtgaccgga ccggcgtgga tcttggtcgg gtccatcatc cggtagccga tgatcacggc
     9061 gacgtaggag accgcgtcca cgatgaagca ccagccgtag cccaccgtcg agatgagcag
     9121 gccggcgagg gccgggccga cgacgcggga accggtcatc agcgcgctgt tgaggctgac
     9181 ggcgttgttc acgtgctcac cgtcgaccat ctcgacgacg aacgaccgcc gagccggatt
     9241 gtcgaacgcc gtcgccacgc cgccgaagaa cgcgatggcg tagatcgctc cgaccggcgg
     9301 gcggtccatg aacgcgagcg ccccgagtgc gaacgactgc gccatggcga acatctgcac
     9361 ccgcatgagg agcttgcgct tgtcggaacg gtccgcgacc aggcccgccc acggaccgag
     9421 gagcagcacg ggcgcgaact ggaaggcggt gagcagtccg acggcgacgc cgttgccggt
     9481 gatcttgagc acgagcagcg cctgggcgac cagcgtgagc cagttgccca cctgcgagat
     9541 ggactggccg gcgaagaaga ggcggaagtt gcggatggag agggacgaga aggtttcgga
     9601 ggacaggagt cggaggcgtg tcatgggttc gttcgttcgt ggttcgtcgt gggtggttgc
     9661 ggaggtgtca ccaggtgctc gagcaccagg gtgacgtcgt ggaggcgagc gacgtcgtcg
     9721 gcggggagct cgcggaggcg agccgacaac cattcggtgc ggcggccgcg gatctcctcg
     9781 atctgctcga tgccggccgg ggtgatggca acgcgggtga cgcggcggtc ggcggggtcg
     9841 gagatccgct cgatgaaccc ccgctgctcg agcttgtcga cgagcttgct gaccgtcggc
     9901 ggggtgacct gctcgtgctg tgcgagatgg ccgagcgtga ggggcccgtg cttccacacc
     9961 gacaccatgg cggtgttcag cgcgggcgac atcccgctct ggtcctgctg gcgcaggagt
    10021 cgggccagcc gagcgatcga catgcgcagg gacgccgcga cctcgtcgat gtcgggagcg
    10081 ttgctctcgg aggcaccggg gttcgagatc gtgggggtcg tcctgggcat ggatcccacg
    10141 atacccgcat tagttagcca gggaaactaa ctaccttggg acagcggcca caggcggcga
    10201 cggccgctgt cgggcacagg agtccgtccg cccgacatcg gcgcggcgat ccgcggcatg
    10261 atcggcgcca tgaccacgtc accctccagc aacttccccg ccgacgaggt ggaggccgcc
    10321 ctcgccacct acgtcgagct ccgcgaacgc atcggtcggc gcgaagccga ctggaccgac
    10381 ctcggcgacc tcttcaccga cgacgccgtg tacatcgacc cggcctgggg tcgactccag
    10441 ggcaacgatg aactgcgccg cttcttcgac gagtccatgc gcggcctcga ggactgggac
    10501 ttccccatcg agtacacggc gatcaccggc gacaccgtcg tgatcaagtg gtggcaacag
    10561 ctccccggca cccggcccga cggcacccgc taccagcagt ccggtatctc caccctcgtc
    10621 tacgcgggcg acggaaagtt ctcctacgag gaggacctcc tcaacatgac ccacgtgctc
    10681 gaggatctcg ccgcgagtgg ctggcgcccc gggaagggct tcatcgcgcc cccggcccaa
    10741 cccgaccgga acttcgcccg cccttgagcc aggctgccct catggcactc gacgacgaac
    10801 tcctcaaccg cgtcacctgg aagatcccca acgccctcgc gctggtcgga tccgccgcgg
    10861 gcggtcgacg caacgcgatg accacgagct ggatcaccca gctcgcccag gagccggtgt
    10921 tgatcggtgt cggcgtcgac aacgactcgg tgaccgtcgg cctgatccgc gacggcggtt
    10981 cgttctcgat caacctctgg gacgccgaga acaccaaggt gttcgtgaag ttctccaagc
    11041 ccgccgtcga cgaggacggc acgctcaacg gccgcgccgt gaccacggcg acaaccggcg
    11101 cgccgatctt cgaggaggcg atcgcgtgga tggactgcga ggtccgccac accctcgacc
    11161 tcggcagcca cacgctgttc gtcggcgagg tcgtcgctgc cgcgatcccg aacgacgagg
    11221 cccgcccggc gtcgatgagc gacacgcgca tgaagtacgg cggcgtccgc cgccactgag
    11281 ccgtggggcc acgctcgcgg gtaccgtgag cgcagacgca agggggacga tcatgaccga
    11341 cacgacgacc accgtcggca agctggccga cgccaccgac cgggtgcccg gttcggggct
    11401 cgcggcgatg cggtttcgca tggaaccgtt cacggtccag gtcgggccga ccacccacct
    11461 cgcggccgag agcgaacgac tcgccggcct cacgttcgag cacttcgatg tccggcgcat
    11521 gggcgccacc atcggcgccg agttgcacgg catcgacctg accgacgatc tgccgcaggg
    11581 agccatcgac gagatcgcca ccgcgctcgc cgagttcaag gtgatcttct tccgggatca
    11641 accgctcacc gccgaacagc acgtcgcgtt cgctcgccgg ttcggcgacc tcgagatcca
    11701 cccgttcatc ccggccaaca ccggccagcc cgaactcgtc cggctggcga agtcggcgga
    11761 gatcggcggc tacgagaacg cgtggcacca cgacgtcacg tggcgcgagg ccccgtcgat
    11821 gggcgcagtg ctccacgccg tgcaggtgcc cgacgtcggc ggcgacaccc tgttctgcga
    11881 catgtacgcc gcgtacgagg gcctcgacgc ggcgaccaag gccgagatcg atcacctcga
    11941 cgccgtgcac gactacatgc gcaccttcgg ccacttcatc cccgacgaca agaaggccga
    12001 gacccgggcg aagtacccgc aggtcaccca cccggtggtg tgcacgcatc cggtcaccgg
    12061 gcggcggcac ctgtacgtga accgcaactt cgtcgaccac gtcgccggcg tcacgcccga
    12121 tgagagcacg gcgctgctcg accggctgtg ccgtcaggcc gactaccccg aataccagtg
    12181 ccgcttccgc tgggaggacg actccgttgc gttctgggac aaccgggccg tccagcacta
    12241 cgcgtcgagc gactactggc cccacgtccg ggtgatggaa cgagcgtcga tcatcggaac
    12301 gcgcccggtg cgctgagccg cccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnntcggcc tcgtcgaacg ggaccgcgtc gaggtgggag cggtacgcgt ccttgaaggc
    12481 cgtgacgtcg tcgataccgg cgaagtcgta gaacgctgcg cctgcctcgg aagtcacctc
    12541 gtacagccgg cgcaccaggc ggccgatgta ctgaccgccc gacaggtcgc ccatgtagcg
    12601 ggtgtagtgg tgggcgatga acccgcccgg ccagtcggcg acctcgccga gccgggcgac
    12661 gtaggcctcg gtctcgggag tcgctgtggc gtcggcgcgc cagtcctcac cgaggaggac
    12721 ctcgaggtcg gcctcgatgc ggggcatccg gatcagggcg gggtcgagga agggcgccac
    12781 ggtcgggtcg ccggcgagac gctcacccgc gccctcgagc gctccgtaga cgaaccacat
    12841 ctgcacgatc atctcggcgt actcgtactg ggtgaggctg cccgccgtca gctcatccat
    12901 gtagccggcg tgctcggcct tgccgtggtc gacccaggtc gcctggcgga tctgttgggc
    12961 gaacccctcg ggggcgatca cggtctcgtc ggtctgcgtc acggagcgtc tccagagtcg
    13021 gggcacagtc gggccaccag aagttgtaag gggtgccaaa cactatacga tctcgtctcc
    13081 gtacaaaaga ggggttcgga cagaacccgg aaatgggaag ctgcaccaca tgaccgacgc
    13141 accccaccag ttcgacgagg ccaccatcgc cggggtgacc gcccacatga acgccgacca
    13201 cggtgccgac acgctgctca tctgtcgcag cctcggcgac cgacctgacg cgaccgccgc
    13261 caccatgacc ggactcgacg gcgacggcgg cgactacctc gtcacgctcc ccgacggcga
    13321 cgagacgatc cggatcccgt ggagccgccc gctcaccgaa cggggcgaga tccgcgtcga
    13381 ggtggttcgc atgtacgagg cggcgtgcgc acagctcggc gtcgaacccc gaccccactg
    13441 accaccacga ctgggcgccc cgccgggcac cccacgcgaa cgacccccac ggtgtcccgt
    13501 gggggtcgtt tcgagtcggg gtgggcggag aatcaggtcg ggacgggcgc cgcgaccgcg
    13561 acccgctcga ggagctcctc gccggcgttg gacgcaccgc tgccggggta caccgcgaag
    13621 ccgtatccgc cgcccgccgg atcgaaggcg gggacggtga tctgcgccgt ccacgaccca
    13681 tcggcgccga tggtgacgaa ctcggcgggg tcctgattcc acgggaaggc caccagcgtg
    13741 tcgatcgacg actgcgggag cgcccacttc tggagcagga cggtgcgccc gccggcgccg
    13801 gccgacggct tccacacctc ggcgaaccgg ccgaacacca cgtacacgcc ggccggggcg
    13861 ccggcgagcg ggggccgggt tccgatgttg ccggtgggcg tgaagccggt tccggacacg
    13921 gtgatgacat cgcccggctg gatcccgacc gtcttcgaca cggtgatggc ggcgccgacg
    13981 gtcggcggca ggcccggatc ggggaaacat ccggtgaggc cgacggcgag gacggtcgct
    14041 ccgacgaagg ctcggagccc ccgtcgcggt cggtcggtgc ggagggtcgt ggcggtggtc
    14101 atgtagtgcg ctcccagtgt gttcgctgac ggacgaacgg tagtagggca cactccactc
    14161 ttgcaatagg tgtgcctaac atccacctcg gaaccaaggc gccatcagta gccgggagcc
    14221 ggttgccaga ggtggaactc ccgtccctgg tcgtcacggc agaccacgtc gccgcccgac
    14281 tcgtagtccg actcggagac gaccgagccg cccagctcga tcacctttgc cgcgaacacc
    14341 gacgtgtccg ggacccggaa gtagagcacc ggcgcactgt cgacgccctc gggggtcatg
    14401 cccatcggga gctcggtgtt gtcgatgtgg gcatacgggt cgcccatgtt gccggtctcg
    14461 gtccgccacc cgaagagcga cccgtagaac gcccgggcct tcgccgtgtc gtcgaacccg
    14521 atggtgaggt acccgagctc gatcggccgg tcggcctcga cgggcgtcac gggcggggtc
    14581 gcaaccggcg ccgcgaccgg cgtctccacc accgtgaacc cctcgaggtt ttgattgatc
    14641 tcgtcgacgg acggctcggc gatcgtcgtc tggatcatcc accgatgacc gaacgggtcg
    14701 atgaccgccg agctgcgctc tccgtagggc tggtcctccg gctcgcgcaa cccggcggca
    14761 cccccggcga cagcgcgggc gtacaccgcg tcgacgtcgg gcaggttgag gtggagcgtg
    14821 accgaggaac ccgacgggtc cggcggaacg accgccatct ccggatacgg atccgacagc
    14881 atcaacctgg cgccgccgat cagcatctcg gcatgcccga tccgaccgtc gtcgccggcg
    14941 taccgcaccg tctcgaccgc ttcgaacgcc atggcgtacc agtcgagggc ggccgccgc
//
LOCUS       DAOSOA010000035        14919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4033_length_14919_cov_7.011572, whole genome shotgun sequence.
ACCESSION   DAOSOA010000035 DAOSOA010000000
VERSION     DAOSOA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14919
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4033_length_14919_cov_7.011572"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..301)
                     /locus_tag="PKY13_04025"
     CDS             complement(17..301)
                     /locus_tag="PKY13_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79126.1"
                     /translation="MTMKPGDWVAIDGYDADDASMAVVVATSRSGERLDEEAVAKIAS
                     GHDHQPVRAVTWHAVLVADHDNIGDDNCEFHEHGTGTAWVEAILCREVIE"
     gene            complement(506..775)
                     /locus_tag="PKY13_04030"
     CDS             complement(506..775)
                     /locus_tag="PKY13_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79127.1"
                     /translation="MGTPFDSTIVPTKAGQRVAFLGHGGDVRQWMCDMTATVVRFNRN
                     GHPVLALDPTASLRAQTGRDSFEVTDRWGCARLLDENDRRWARPA"
     gene            complement(775..1173)
                     /locus_tag="PKY13_04035"
     CDS             complement(775..1173)
                     /locus_tag="PKY13_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79128.1"
                     /translation="MTAPATLSRYRLVQRSDHYQFDAADDETATLLVCLLGEAVGWRC
                     EDRWFLNLLCDPEELEGILGLKMADALGTYGHRLVDALLTVEIEPDLRRRSGVDPDTW
                     HERHVGGNFDYRAVARRLATTIGDRLTQSS"
     gene            complement(1358..1852)
                     /locus_tag="PKY13_04040"
     CDS             complement(1358..1852)
                     /locus_tag="PKY13_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79129.1"
                     /translation="MNDTTSCDGRVTDDAHDAIVNLIYRVDQLLSAPDLGTAAERLVA
                     VSNANSDARSWFKAHWDERGDWIEGSDGDNGDCPVQPELALYEYTMTNPDTDLGWSSG
                     FVRAPHDEAAVAFAASWPTANGWPTGTKWSVARVAALLTVDENGQPCSGSAMISTIAD
                     GVTS"
     gene            1952..>2154
                     /locus_tag="PKY13_04045"
     CDS             1952..>2154
                     /locus_tag="PKY13_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79130.1"
                     /translation="MTEHRASRSLRFSETVRVLSDSARRQGLEVPMFRSPCRNPEHTR
                     TLRRRPDGVVVAVRLSDRPFAAV"
     assembly_gap    2155..2254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2362..2787)
                     /locus_tag="PKY13_04050"
     CDS             complement(2362..2787)
                     /locus_tag="PKY13_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79131.1"
                     /translation="MNTEPFGDLDDGQVLVGAESNDLVGRHVEPWPTGFPDGDARLDE
                     TCPDNTRVDADLFGDFGYRKLLVYVETHRLGDVDRRPRSAARVLRRPHFDACAAQVRC
                     DCRSLDAESFADLGERELLVDVESFQLVDIEVEPVGFTT"
     gene            2881..3030
                     /locus_tag="PKY13_04055"
     CDS             2881..3030
                     /locus_tag="PKY13_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79132.1"
                     /translation="MIETALASSGLDLARFTELGRTDQLVDPNLRDLWLIWGAELTDI
                     RPAAR"
     gene            6765..>7951
                     /locus_tag="PKY13_04060"
     CDS             6765..>7951
                     /locus_tag="PKY13_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79133.1"
                     /translation="MYVVDVRSAPPTPVRCISIAHPDSTFLVEGFVRTHNSSAVRSIW
                     GWNCVERVAPSGTPGFPGERNALVAFESKPDGAAWYRRWSEGVGDEVSIIEVGDPSTW
                     SIDLLGVPGTAEERARFFVNAMVYAFNDGAIQDRSFETLTAVLTAALCVTPSIAAAAG
                     VSPDGSPLFFAHVLLGGLGDPMAVALAESLASEATRSEREAGSRSLPAAGGTQGAFVP
                     LTEIGMADQKLMPMFGGRSTEATRRSLCEAPRNKLGQLLGAESWWSPARVKVTWDQVL
                     EHHLAVVVNTGVSTSGAVVDERLSGQMSALLMYGLRDAIQRRCSGWQEEGRSVSIFAD
                     ELALLVGSSADVVTWLRNQGRSYGVRATFATQYPEQLPRPVRTVFMSFDTFVWFAQTN
                     AEVV"
     assembly_gap    7952..8051
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8052..9134
                     /locus_tag="PKY13_04065"
     CDS             <8052..9134
                     /locus_tag="PKY13_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79134.1"
                     /translation="GRRGGVTRAGQRGAPASHRHEGRLASLAAVGMWRTLSAQQVVAM
                     TGFGLGPALTESMSALWAAGLVERGELVSGMVGSRLPALFRACDSTAVDAFLADLPYR
                     EWVAVTGGQPWTLGPRHDRHNMLTTELGLRIAEYCEVSAVLGESMAGFHLLDPSGATP
                     TSTTTAADAVVIRPDGLRVCLETTANAGSSFERKVDRWVEFLMGADHDDLGAVVCFVE
                     SARPDDRNANDLFRTVRRTVERAVGARSGAVRRRLAQRMFVARWRWWFPAPSSATAGF
                     RTLVAVCPDGTGRDNRWREAHMLDLTDVTFDPSPALERLEVDFSTLLGTPFWLRQGEP
                     VDLGPVIVSQAGLTELPVPVAVGSRR"
     gene            9236..9814
                     /locus_tag="PKY13_04070"
     CDS             9236..9814
                     /locus_tag="PKY13_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79135.1"
                     /translation="MDYCAYSAGGRASCLGEDGGEVFGLRVVLNTIAVLGVRGFMVLA
                     VLILGGIGLYAHFDDGPVNQGDRAVINERAKNAVVGDLYEATISSEAGVSCVITPASL
                     TVKRGDKITFKSATRVAVGAPTDHVEDFVHLVEVDGSYQVTVPVDEAGPFECDDEQFL
                     EQFNALRSGNDAAKIAQLNDAYSHGLLTVSFG"
     gene            complement(9971..10648)
                     /locus_tag="PKY13_04075"
     CDS             complement(9971..10648)
                     /locus_tag="PKY13_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79136.1"
                     /translation="MAREYDALLARIDDPALRDDLRRSFDKVRAKRTFGLVFESHLPE
                     RVTLPSHPIRRGVRVARRDDQRQVFFVGTVKRGIAQLIPIGTDDDSPNDQPTELPVGE
                     LVVVAEFGEPIYPGLKHLESVHRGGDKPAHTVINAENHHALELLQFTHAGKIDCIYID
                     PPYNTGARDWKYDRTPLVRVEALADIGGTIRALDLLDPAVRTAVRAFDGGDTRGLYAS
                     SHGRDYR"
     gene            10777..11622
                     /locus_tag="PKY13_04080"
     CDS             10777..11622
                     /locus_tag="PKY13_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79137.1"
                     /translation="MSTSQSRKPRGIPTGGQFSSMGRSESDVELDGEPVDEFVPSYSS
                     WRHGGWYVDNVRYPSGAVGCVSRNYEDRKWRIVCDPRPFEDAPTFKSRDDAARAELEL
                     TERHAAAPCTCPPPPAEVDERLHHLHQSNDCSRHGRSGAAWRAVEEFEAQEAATHENS
                     GANPAEAAPQTCTVTNSDGRSFTAHLVRIGESDETTTKAIVEIYDDTRAGDPRFGPRG
                     QRASSYYVDTFNEVNGGLNLDGGVPVWQIGPDEVRAIQGWLNEVGADGQRSEDDPFTT
                     DQAER"
     gene            11935..13299
                     /locus_tag="PKY13_04085"
     CDS             11935..13299
                     /locus_tag="PKY13_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79138.1"
                     /translation="MSSLHRQPRGLPIGGQFAAVARDESDVLLAEPVDETAEERWVSL
                     LGPRARAQLTRAGVTAEWLEAAEQLDSQHGLRATGDYQPLIAAKRAGFTPAQAAALFE
                     VCGPSGYFSTAEPPWTDSDLLRSLPEGLRPIPFDMVPRLVAPGVDHVAVEVAAQHVSD
                     GIRRSSLKMALDQALDIAAAGITQADADATGSGDDWWRAYLCRRDGVPDEDAIAWAPS
                     YPHSIRLFQEAGWSPDQVNDDNRLIAAAWSDAGVSPAAMSEWNAAGFDHRHEASRYLS
                     AGVGPAEAAQWRAIYPTIDRDVATYRAAGVDVAAVGRAHAAGLELDPSLAPAIIAAVP
                     PDELRQWATAFGGRIRSGTITAYRNAGVVTPSRAGAWAAAAMGSAADKRDTITPATLG
                     FINRHVDPPEVGGFEVAPIVTATAVELGLDPVEVQRRAEKSGGGLVEGCRTMFRRRRV
                     DPPF"
     gene            complement(13709..14053)
                     /locus_tag="PKY13_04090"
     CDS             complement(13709..14053)
                     /locus_tag="PKY13_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79139.1"
                     /translation="MTTHFGRSPSLAWLYRRDTLETLSSHPWLAEFSRAAGCQNTDDV
                     EDVVKHLASNSGAVGLLDDRKVRALVLELITPTKQTDELGRIVKPHHPGTSEWAEILD
                     DAITTAEVSFHD"
     gene            complement(14574..>14919)
                     /locus_tag="PKY13_04095"
     CDS             complement(14574..>14919)
                     /locus_tag="PKY13_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79140.1"
                     /translation="HDDLDHLDGASDLDDHHDLDHLDGAADHDDLDHLDGASDLDDHH
                     DVDHLDGASDLDDHQHDDGGASDLDDHQHDDGGASDHVDHEHDDGGASDLDDRETARL
                     ADDLLLGLHGQH"
BASE COUNT         2171 a         4480 c         5435 g         2633 t
ORIGIN      
        1 aagatcaggg cggcggtcac tcgatgacct cgcggcacag gatcgcttcg acccacgctg
       61 tcccggtccc gtgctcgtgg aactcgcagt tgtcgtcgcc gatgttgtcg tggtcggcga
      121 cgagcacggc gtgccaggtg acggctcgga ccggttggtg gtcgtggccg gaggcgatct
      181 tggccacagc ttcttcgtcg agccgttcgc cgcttcgtga tgtggcaaca acgacggcca
      241 tcgaggcgtc gtcggcgtcg tacccgtcga tcgcgaccca gtcgcctggc ttcatggtca
      301 tgtgattctc ctgcgttggg gccgagtgtt ggcagctcct atgtgcgccg gttcggcccg
      361 gctcccatcg accgcggatc tatgtgcatc gaaaggcgtc tatggccggt ggatcagctc
      421 gtaggtggcg ggttcagctg ggtgggtgag acgtccggtt cggagacgag acgcatgcat
      481 cggacggcgc ctgatccgca ccggatcagg cgggtcgagc ccaccgtctg tcgttctcgt
      541 cgaggagtcg agcgcaaccc caccggtcgg tgacctcgaa gctgtcgcgc ccggtttggg
      601 cgcgcagcga cgccgtcggg tcgagcgcga gcaccgggtg tccgttgcgg ttgaaccgga
      661 cgacggttgc cgtcatgtcg cacatccact ggcggacgtc gccgccgtgc ccgaggaacg
      721 cgacccgctg gccggccttg gtcgggacga tggtggagtc gaagggcgtc cccatcaaga
      781 gctttgggtg agtcggtcgc cgatggttgt tgcgaggcgg cgggcgacgg cgcggtagtc
      841 gaagttgccc ccgacgtgac gctcgtgcca ggtgtcgggg tcgactccgg agcggcgccg
      901 aaggtcgggt tcgatctcga cggtgaggag tgcgtcgacg agacggtgcc cgtaggtgcc
      961 gagcgcgtcg gccatcttga ggccgaggat cccttcgagc tcttcggggt cgcacagaag
     1021 gttgaggaac cagcgatcct cgcatcgcca tccgaccgct tcgccgagaa ggcagacgag
     1081 cagggtcgcg gtctcgtcgt cggcggcgtc gaactggtag tggtcggagc gttgcacgag
     1141 ccggtagcgg gacagggttg cgggtgcggt catggcgctt atgtgcttcg gggatgtccg
     1201 gttcccgccg acggtcaacg ccggtgccgg ccgtgagcac cgacgggggt ggtcgttcgg
     1261 acgatgacgc cgacgggttg gccggtggcc ctcactcggt agcggcgggt gtcgccggct
     1321 cgggtgtacg tgatgtcggt ccggctgatg cgctcggtca cgaggtgaca ccgtcggcga
     1381 tcgtgctgat catggcggaa ccgctgcacg gctggccgtt ctcgtcgacc gtgaggagtg
     1441 ctgcgactcg ggcgacggac catttggttc cggttggcca gccgttggca gtcggccacg
     1501 atgcggcgaa cgcgaccgca gcctcatcat gtggggcacg aacgaacccg ctcgaccagc
     1561 cgaggtcggt gtcggggttg gtcatcgtgt actcgtacag tgcgagctcg ggctggacgg
     1621 ggcagtcgcc gttgtcgccg tcggatcctt cgatccagtc gcctcgctcg tcccagtggg
     1681 ctttgaacca ggaccgtgcg tcgctgttcg cgttcgacac ggcgacgaga cgttcggcgg
     1741 cggtgccgag gtccggcgcg ctgagcagct ggtcgactcg gtagatgagg ttgacgatgg
     1801 cgtcgtgggc gtcgtcggtg actcggccgt cgcagctggt ggtgtcgttc atgcactcca
     1861 tgtgcaccgg gtcgaggtgg ctcccgtcgg gtggggatag gtcgcggcct gggcacatag
     1921 gtgtcgacga cgacgtcgaa aggaacgaac catgaccgag catcgcgcca gccgctccct
     1981 ccgcttctcc gagacggtgc gtgtgctgtc cgactcggcg cgccggcaag gtctcgaggt
     2041 tccgatgttc cggtccccgt gccgcaaccc cgaacacacc cgcacattgc gccgccgccc
     2101 cgacggtgtc gtggtggcgg tccgtctctc cgaccggccg ttcgccgcag tgcannnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgtacg tcgacgaagg actgtccatc
     2281 cagcaggtcg tggatcgcac cggcgtgaaa cgccaaaccg tatctcgccg gttgcgcgca
     2341 gccggcgtcg cgctgcgacc gtcaggtggt gaagccaacc ggttcgacct cgatgtcgac
     2401 gagctgaaac gactccacat cgacgagcag ctcacgctcg cccagatcgg caaacgactc
     2461 ggcatccaag gagcgacagt cgcagcgcac ttgcgccgcg caggcgtcga agtgcggccg
     2521 acgaagcaca cgggcggccg accgcgggcg acggtcgacg tcgccgaggc ggtgcgtctc
     2581 tacgtagacg agcagcttcc ggtagccaaa gtcgccgaac aggtcggcgt ccacccgggt
     2641 gttgtccggg cacgtctcat cgaggcgggc gtcaccatcc ggaaagccgg tcggccacgg
     2701 ttcaacatgt ctgccgacga gatcgttcga ctccgctccg acgagaacct gaccgtcgtc
     2761 cagatcgccg aacggttcgg tgttcatccc ggcaccgtcg gggaacgcct cctcgaaggc
     2821 ggagtcaacc tccgctcggc ggtcacagtc cgaaccgtca cccgctccga aatcgttgcc
     2881 atgatcgaga ccgcgctcgc cagctccgga ctcgacctcg cccggttcac cgaactcggc
     2941 cgcaccgatc aactcgtcga cccgaacctg cgcgacctct ggctgatctg gggcgccgaa
     3001 ctcaccgaca tccgacccgc ggcccgttga gatccgaggg cgggcccgca gctgggcaat
     3061 gtcacccgca ccgttgcaac cccggttcgc tcttgtacgg tgaacctcgc aagccccggg
     3121 atggctcgtc gcaagacggc caccggggct ttgtccattc tcggccctcg gaccaagcgc
     3181 ggctcaccgg accagcgttt tcaccgaccc gcccgccacc accacaacga ccgaccgagc
     3241 gtcagcaacc gccggaacga aagtgccggc aaccccgggc ggtagcccta aaagaacctc
     3301 gcttcgctcg gttgggcggc gcgggttcgt ttcgttgtcg gggtgttgtc gcggttcgtt
     3361 tcgtggtggt tgcggttcgt tggtcggtgg tggttggtgt tggtggttag gttcgggggt
     3421 gtcgcatccc gggggcctgt ggcggcgtgt gcggcggcca gggttccggg gtgtggcggc
     3481 ccacgtgggg gcgtttcgat tcgtggcgtg gtcggggcgg tgacgtgacg ccggcggggg
     3541 cggttcgtga cggccggcgg ggcgggatcg gatcggattc gtttcggaac cggtggacac
     3601 ccaccggaac caacccacac atgccgtgac cccaccgagg gttgccggcc gaaggcaggc
     3661 gaccccacaa ccttgctcac cgtcaggggc acccgcaggg tgagccaacc tccagccacc
     3721 tcaacctcaa cgcgcccaac aacccccagc acagcaacct ccaacagcat caacccccga
     3781 cttccaccac cgacaacgcc acaacaaccg gcaccaacag cccccctcat tcccaccacg
     3841 gaacgcaacc cgcagcaccg ggaggggcga cggggcggtg tgggcggcgc tggcggggtt
     3901 cgtcgtcgaa cgcgcgttcg cgtttcgcgc atcgcgactg cggcatgttc agcggggtgt
     3961 ctacgcatgt tctgggcatg gcaactgcga cgcgagtgtg gcgtttgacg agggctggcg
     4021 gggggctgcc tgaccctgcc gatttcgcac ggcgggccca ggtgttcact cggacggcga
     4081 agtcgtcgac gctcgtgacg ctccacgatg ggggcgggtt gcggtcgttc gtcgtggttc
     4141 ccgagggcca tgacgcagat caggcggcgc tacatcttgc ccaggcggtc gcggcgcggg
     4201 cggagccggt ggagatgccg gcgctgggtg gcgcggcggt gggttggctg gtggcgcgcc
     4261 cgtcgtcgac ggctgttcgg gatccgcagg ctggcgccga tccggctgag gttgcccgcc
     4321 ggttggcggt ggcgatgcgc ccggggagct ggttggcggt gctggtccgt gagtccggcc
     4381 gttcggaggt gcgatggtcg cggcgctggt acgaggcccg cttggggacg acgatgccgg
     4441 tgcatcattc gacggggaag tcggtgacgg tgtcgttctt ggcgggcggt gacagcgcgt
     4501 tcgaggttcg gtcgatgctc gatcaggtgg cgggttcgtt gccggggttc gatgtcgaag
     4561 tggtgtctcg agtgggttcg atgcgccgca cggtggcccc gtggctggtg gcgtcggcgg
     4621 ttgcgtgggc aggggttgtg gtcggcgttg gctggtcggc gttggctggc gcagcggcag
     4681 cgttggcgcc cgcactggtt gggttgttcg tgtcggcggg gcgtgcgggg tcgatggtgg
     4741 gtcgggtgcg caggttcgct gagcaggggg tcttcgttcg tccggcgcgg cggattctgc
     4801 cggcgaagcg tcccagtccc ggttctggga gcggctggtt ggatcggctg ggtgggggcg
     4861 actatccgtt ggcgtcgacg tcgtttctgg tcggcccgca ggtggtcgtc ggtgttgttg
     4921 caccgcatgc tggtgcttct tcgggcgctg cgttgacgga gcgtcgcgcg gcgccgtcgg
     4981 ggctgctcga caggatcggg ccggcgctgg gggtggcggg cgaggcggcc gcgatggttc
     5041 atcttccagc ggagtcggcg tggggcggcg tcgggttggc gggcacgccg ggcagcggga
     5101 aggcgctcga cctgtccagc agaatccctg tgccggtgag cgggcggttc ccttcggggt
     5161 gggcgttgaa cagtgagctc gaagttggtg acgagttgtt cgctggtgac ggttcggtca
     5221 cgacggtgat cgggttctct gacgtgttcg tcggcaacgc gttcgatgtc gagttcagtg
     5281 acggacaggt tgttcgatgc gatgccgacc atctgtggaa ggcgtcttcc cgaaagaagc
     5341 gccagcgtgt ccagcgggcg actacggcag ggatccgtgc ttcgaattcg gctgaggtcg
     5401 agcgacttcg ggcgcttgct cggggggtcg ctcccggcac gttcgcctca gcgaaagacc
     5461 ttgccgagat gatccactgc tcgccgagcc acgtccgggc gtcggccaga gcgttcggta
     5521 tcgaaggtgc cgagaccctg atggtcgtcg agcccgctcg cttctcccgc cagaaacggc
     5581 atcgccgaga atgggccgcc gacgacgtcg cggccgtgat cgaatcgttg tcggcgagca
     5641 aatggtctcc gatacagccc gatgattccc ggcgcttctc ggaccgggag tggtggtgcg
     5701 ctagcgacgc gttcgttcgt acgttcaacc gtcctccggt tggtaacgac gtagaggtga
     5761 tgagggcacg cttgtcgagc tcgggtgcac cgtcacgaac agttgccgtc ctggcggaca
     5821 tcgacttcag ggcgacggtg aagtcggtca tcacctaccc gatggccgag gtgctttggt
     5881 cgttcgctga ccggctcgaa caggcggagg ttccgctccg cgagtccgaa accatcgtgt
     5941 cgaccaagga tctggcggac gcagtgatgg agggcgggca ccggaattgg gctgtgcgcg
     6001 tcggcgaaac gatcgaactt ccggcctcgg atcttcgtgt cgatccctac gtcctgggtg
     6061 cctggcttgg cgacggcgac acggacatcg gggcgttgac gtgcggcgcc caggacgtcg
     6121 acgaggtcgc ggctcggctc ggcgacatct ggggtccggt tgtggtccga gagacgtcgt
     6181 cgggtgcctt ccgtttgacg ctcccgcggc ctcggcgtga cctctgtggg tacggtcatc
     6241 cggtgacgca ggaagcgggc cggccgtctc gaacggcgcc atgctcggag tgcgttcggg
     6301 tgcgcggccg gcgccggagt gccgggttga ctggccgcgc cgtcgacggt gtcgagcgat
     6361 ggaacgttcc gctcaccacc gctctcggtg agctcggcct gttgggcgcc aagcacattc
     6421 ctgcgaccta tttgcgcgcg tcagctgctc agcggcttgc cgttctccag ggtctcatgg
     6481 acacggacgg ccacattgat ccgaatggct cgtgcgagct cacgctgtgc aatgagcggc
     6541 tggcgaccca cgctctcgag ctgattcgct cgcttggcat caagggttcg atgcgatcgt
     6601 cggatgcatc catcaccgaa cccgaccctg accggccggg ctacaagcga cgacggatcg
     6661 tcggaacccg ctggcgcatc gtgttcacaa ccagccagcc cgtcttcagg ctgaggcgga
     6721 agcttgagcg tgttccgacg aagacccgag cgacgcagca ccacgtgtat gtggtggacg
     6781 tgcggtcggc accgccgaca cccgtgcggt gcatctcgat cgctcacccc gattcgacgt
     6841 tcctcgtcga gggcttcgtg cgaacacaca actcgagcgc ggtcaggagc atctggggtt
     6901 ggaattgtgt ggagcgggtc gccccgtcgg ggacgcccgg ctttccggga gagcgaaacg
     6961 cgctggtcgc gttcgagtcg aaaccggacg gggcggcgtg gtatcggcgc tggtctgaag
     7021 gggtcggcga cgaggtctcg atcatcgagg tgggcgaccc gtcgacgtgg tccatcgacc
     7081 tgctcggtgt gcctgggacg gccgaggaac gggcccggtt cttcgtcaac gccatggtgt
     7141 acgcgttcaa cgacggcgcg atccaggatc gcagcttcga gacgttgact gcggtgttga
     7201 cggcggcgct atgtgtgacg ccgtcgatcg cggctgcggc gggtgtgtct ccggacgggt
     7261 ccccgctgtt cttcgcccac gtccttctcg gtggtcttgg tgacccgatg gcagtggctc
     7321 tggcagagtc gttggcgagc gaggcgacac gctccgagcg cgaggccggt tcgcggtcgc
     7381 tcccggctgc gggcggcacc cagggtgcgt tcgtgccgtt gaccgagatc gggatggcgg
     7441 accagaagtt gatgccgatg ttcggtggac gatccaccga ggcgacgcgc cggtcgctgt
     7501 gtgaggcacc gcgcaacaag ctgggtcagc tgttgggcgc cgagtcgtgg tggtcaccgg
     7561 cccgggtgaa ggtgacgtgg gatcaggtcc tcgagcacca cctggccgtg gtggtcaaca
     7621 cgggcgtgtc gacgtcgggc gcggtggtgg atgagcggct gtcgggtcag atgtcagctc
     7681 tgctcatgta cggcctgcgc gacgcgatcc agcggcgctg ctcgggctgg caggaggagg
     7741 gccggtcggt ctcgatcttc gcagatgagc tcgcactcct ggttggttcg tcggccgatg
     7801 tggtgacgtg gctccgcaat caggggcgct cctacggcgt gcgggcgacg ttcgcgacgc
     7861 agtaccccga gcagttgccg cgcccggtgc gcacggtgtt catgtcgttc gacacgttcg
     7921 tgtggttcgc ccaaacgaac gccgaggtgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nggccgtcgt ggtggtgtga ctcgagcggg ccagcgcggc gcgccggcgt
     8101 cgcaccgcca tgagggccga ctggcgtcac tcgcggcggt cggaatgtgg cggacactgt
     8161 cggcacagca ggtggtggcg atgaccggct tcgggttggg cccggcgttg accgagtcga
     8221 tgtcggccct gtgggctgca ggccttgtgg aacgcggcga gctcgtgtcg ggcatggtcg
     8281 gttcgagatt gccggcgctg ttccgggcgt gcgattcgac cgcggttgac gcgttcttgg
     8341 ccgacctgcc ctatcgggaa tgggtcgcgg tgacgggcgg tcagccgtgg acgttgggcc
     8401 ctcgccatga ccgccacaac atgttgacga ccgagctcgg attgcggatc gccgaatact
     8461 gcgaggtgtc ggcggtgctc ggcgagtcga tggcggggtt ccatctgttg gacccgtcgg
     8521 gggcgacgcc gacgtcgacg acgaccgcgg ccgacgcggt ggtgatccgt cctgatgggc
     8581 tgcgtgtctg cttggagaca acggcgaacg cggggagcag cttcgagcgg aaggtggacc
     8641 gctgggtgga gttcctgatg ggcgccgacc atgacgatct gggcgcggtc gtgtgcttcg
     8701 tcgagtcggc ccgtcctgac gatcgcaacg ccaacgacct gttccggacg gtgcgtcgca
     8761 cagtggaacg tgccgtcggg gcacgctccg gtgcggtgcg ccggcgactg gcgcagcgca
     8821 tgttcgtcgc ccggtggcgg tggtggtttc ccgcaccgtc gtctgcgaca gcgggcttcc
     8881 gtaccctcgt tgcggtgtgc ccggacggga cgggccggga caaccgttgg cgggaggcgc
     8941 acatgttgga tctgaccgac gtgacgttcg atccgtcgcc ggcactcgag cgtctcgagg
     9001 tcgacttcag cacgttgctg ggcacgccgt tctggttgcg gcaaggtgag cccgtcgacc
     9061 ttggcccggt gatcgtcagc caggctggcc tgaccgaact accggtgcct gtcgccgtcg
     9121 ggtcacgccg gtaggtgctc gggatttggg tgcccacacg gaggcccggt gagcgagcga
     9181 cggcagattg aggcctgcgt cgggcgattt cgatgccgct acgggatcag gacgcgtgga
     9241 ctactgtgcc tattctgccg gcgggcgagc gtcgtgcctt ggcgaagatg gaggggaagt
     9301 gtttggtctc cgggttgtcc tcaatacgat cgccgttctc ggcgtgcgcg ggttcatggt
     9361 gctcgctgtt ctgatccttg ggggaatcgg gctctacgcg cactttgacg acggacccgt
     9421 caaccaagga gaccgtgcgg tgatcaacga acgcgctaag aatgccgtgg tcggggactt
     9481 gtacgaagcg acgatctcgt cggaggcagg cgtttcgtgt gtcatcactc cggcgtcgct
     9541 gacagtgaag aggggcgaca agatcacctt caagtccgcc acgcgtgtcg ccgttggcgc
     9601 ccccacagat catgtagagg acttcgtaca tctggtcgag gttgacggct cctaccaggt
     9661 gacggttccg gtcgacgagg ccggaccgtt cgaatgcgat gacgaacagt tccttgaaca
     9721 gttcaacgca cttcgctcgg gcaatgatgc tgccaagatt gcccagctca acgacgccta
     9781 cagccacgga ctcttaacag tctcgttcgg atgagaggat cagacctaga cgcctatcca
     9841 acatctactt gaacgcgggg ccggcgcatg tcggacccct gtctgtcagg gtgaggcgag
     9901 aagccggctc ctcggatttc agcggggagt gcggggagta gcaggtcgga tccaaccggc
     9961 gactgctggg ctaccggtag tcccgtccgt ggctcgatgc gtagaggccc ctggtgtcgc
    10021 caccgtcgaa ggcacgcact gctgtccgca cggcggggtc gaggaggtcg agcgctcgga
    10081 tggtgccgcc gatgtcggcg agcgcttcga cgcggaccag cggggttcgg tcgtatttcc
    10141 agtcgcgggc accggtgttg tacggcgggt cgatgtagat gcagtcgatc ttgccggcgt
    10201 gggtgaactg caacagttcg agggcgtggt ggttctcggc gttgatgacg gtgtgtgccg
    10261 gcttgtcgcc gccgcggtgc accgattcga ggtgtttgag gccggggtag atgggttcgc
    10321 cgaactcggc gacgacaacg agttcaccga cgggaagctc ggtcggctgg tcgttgggcg
    10381 agtcgtcgtc ggtgcctatc ggtatgagct gggcgatgcc gcgtttcacg gtgccgacga
    10441 agaacacctg acgctggtcg tctcggcggg ccacgcgaac accgcgacgg atcgggtgcg
    10501 agggcagcgt gacgcgctcg gggagatgcg attcgaacac caggccgaag gtccgcttgg
    10561 ctcggacctt gtcgaagctg cggcggaggt cgtctcttag tgcagggtcg tcgattcggg
    10621 cgagtagcgc gtcgtactct cgggccacct gaaccgcctt cgtctccgtg ccgcgcgagg
    10681 gactcacgct ctgcccgaca tcgggtgggc agtgcagccc caccctgttg agtcgtggcg
    10741 ggtggacggc gaccgccagc aacccacatc ggcgctatga gcacttcgca gtcgcgcaag
    10801 ccccgtggta tcccgaccgg cgggcagttc tcgtcgatgg gccgtagcga gtccgacgtc
    10861 gagctcgacg gcgaacctgt cgacgagttc gtcccgtcgt actcctcgtg gcgtcacggc
    10921 ggctggtacg tcgacaacgt ccggtacccg tcaggtgcgg tcgggtgcgt gtcgcgcaac
    10981 tacgaggatc ggaagtggcg gatcgtgtgc gacccgcgac ccttcgaaga cgccccgacg
    11041 ttcaagagtc gcgacgacgc ggcgcgagcc gaactcgagc tgaccgagcg ccacgcggct
    11101 gccccgtgca cctgccctcc gccacctgcc gaagtcgacg agcggttgca tcatctgcac
    11161 cagtcgaacg actgctcccg gcacggtcgg tccggggcgg cgtggcgcgc tgtcgaggag
    11221 ttcgaggccc aggaggccgc gacgcatgag aactccggcg caaacccggc ggaggccgcg
    11281 ccgcagacct gcaccgtgac caactccgat gggcgttcgt tcactgctca cctggtccgg
    11341 atcggtgagt ccgacgagac gacaaccaag gcgatcgtcg agatctacga cgacacccgt
    11401 gccggcgacc cccggttcgg tccacgcggt cagagggctt cgtcgtacta cgtggacacg
    11461 ttcaatgagg tgaacggcgg actcaacctc gacggcggtg tccctgtttg gcagatcgga
    11521 cccgatgagg tccgggcgat acagggctgg ctcaacgagg tcggtgccga cggccagcgg
    11581 tcggaggacg acccgttcac caccgaccag gcggaacggt gacagcacgc cagccgcggg
    11641 gtacgacgac aggcggccgt ttcgccgcga cccagcgggc agaaggcgac gtcgacctgg
    11701 actctgatcg ggactgcctc gagtgcagcc atccacgatc ggcgcactcg ggaacgttcg
    11761 tccctgacga cacgagctgc cagcacgatc tcgacgccga ttacgacgac gagaagggcc
    11821 gattcgtgaa ggtgggaggg tgcgactgtc ccgcgttcgt gtgacgcaga acggcaccgt
    11881 cgcggcgtcg cggcgtcggg agcacgggct cggccagcaa cccacatcgc gaccatgagc
    11941 tcactgcacc gtcaaccccg cggccttccg atcgggggcc agttcgcggc cgttgcgcgc
    12001 gacgagtccg acgtcctctt ggcggagcct gtcgatgaga cggcggagga gcgctgggtt
    12061 tcgttgctcg ggccgcgggc gcgggcacag ctgacccgag ccggcgtgac ggccgagtgg
    12121 ctggaggcgg cagagcaact cgactcccag cacgggttgc gcgccacagg ggactaccag
    12181 ccgttgatcg ctgcgaagcg tgcagggttc acgccggctc aggcggcggc cttgttcgag
    12241 gtgtgcggcc cgagcggata cttctcgacg gcggagccgc cttggaccga cagcgacctg
    12301 ttgcgatcgt tgccagaagg cctgcggccg atcccgttcg atatggtgcc gaggctggtg
    12361 gcgccgggtg tcgatcacgt tgccgtcgaa gtagcggcgc agcacgtgtc ggatggcatt
    12421 cgccggtcga gcctcaagat ggcgttggac caggcgctcg acatcgcggc cgccgggatt
    12481 acgcaggcgg atgctgacgc aacggggtca ggtgacgact ggtggcgggc ctacctgtgc
    12541 cgacgcgacg gggttcccga cgaggacgcg atcgcgtggg ccccgtcgta cccccacagc
    12601 atcaggttgt tccaggaggc cgggtggtcg cccgatcagg tgaacgacga caaccggctg
    12661 atagcagcgg cgtggtcgga tgcgggcgtc tcgccggccg cgatgagcga gtggaacgcc
    12721 gccgggttcg atcatcgaca cgaagcgtcc cgatatctca gcgccggcgt cggccctgcc
    12781 gaggcggccc agtggcgggc gatctacccg acgattgatc gcgatgtcgc gacgtaccgg
    12841 gcggccgggg tcgatgtcgc tgctgtgggt cgagctcacg cggccgggct cgagctcgac
    12901 ccgtcgctgg ctcctgcgat catcgccgcc gttccgcctg acgaactccg ccagtgggcc
    12961 accgccttcg gtggacgtat ccggtcgggc acgatcacgg cctatcgcaa cgccggcgtg
    13021 gtgacgccgt cccgagccgg cgcgtgggct gcggctgcga tgggttcggc ggcggacaaa
    13081 cgcgacacga tcacgccggc gacactgggt ttcatcaacc gtcacgtcga tccaccggag
    13141 gtcggagggt tcgaggtcgc accgattgtg actgcaacgg cggtcgagct cgggctcgac
    13201 ccggtggagg ttcagcgccg agcggagaag tcgggcggcg ggctggtaga gggttgtcgg
    13261 acgatgttcc gccgtcggcg cgtcgatccc ccgttctgag gtggggtgga tcgctcggca
    13321 ggcaacccac atgacgagca tgagttcaca gcaccgtcag ccgaagggtg tccctactgg
    13381 tggccagttc cgacccgcgg gccgcgaaga gtcggcggta accgtcggcg cgtcgatgcc
    13441 ggtgggcgat ccggccccga ccggccggag cgcctatcgg ccgggtcacg cacggcctgg
    13501 tgagaaaact ccgcagagcc atcctgaaga gttctctgtt gaggacctaa aggcggaggc
    13561 gaagcacctg gtgggcctga accgcaggct cggggccaac accctcagaa cgctggccta
    13621 cctggatctg ctgcgggaga ttcgttctcg gcaggggagt gcgccgacct gagcaacgcc
    13681 gggtctggcc gcttcgacgg tggcgcgatc agtcatgaaa ggacacctcc gccgtcgtga
    13741 tcgcgtcgtc gaggatctcg gcccactcgc tggtaccggg gtggtgaggc ttgacgatcc
    13801 gacccagttc gtcggtctgc ttggtggggg tgatcagctc gagtacgagc gcgcggacct
    13861 tgcggtcgtc gagtaggccg acggcaccgc tgttggaggc gaggtgcttg accacgtcct
    13921 ctacatcgtc ggtgttctga catcccgctg cgcggctgaa ctcggccagc cacgggtgag
    13981 atgacagcgt ttcgagagtg tcgcggcggt acagccacgc gagcgaggga gagcggccga
    14041 agtgcgtggt catggcggca aacccagggc gcaggtcagg ttcgattcgt tggcgaatcg
    14101 tgaaggcacc ggatgcgaag tcgggcccct ctgtggcaca ccggcagtct tcgaggacga
    14161 acccttcgcc ggcgagtcgc gacaagaacc ggccgtggag tgcagcgact gtgccgtgag
    14221 cgaccgctat cgcacggtgg ggaaggccgc cgttgagcag gagcgcgagg agcgactgca
    14281 ccgagccctg ctcgcgcggt tcgaagagcc cgaccagcgc ctgggcgtct tgatggtccc
    14341 tcctcgtcag agggcgtgct gttgggacgt aggggatggg cacggccgcc gctcctcgtg
    14401 ggggaaggtc aaccaagagt gccgcgaaac ttctatgttc gcatgagtag atcgctctat
    14461 gtgcgggtct ggacgccagt cggcgcagtg cctcggggca ctacttcgag cgtttggcga
    14521 gcattcggag ggcaccgccg agaacgagca ggccgagagc gaacgggacg agctcagtgt
    14581 tgcccgtgta ggccaagaag gaggtcgtcg gcgagacgag cggtttcgcg gtcgtcgagg
    14641 tcggaggcac cgccgtcgtc gtgctcgtgg tcgacgtggt cggaggcacc gccgtcgtcg
    14701 tgctggtggt cgtcgaggtc ggaggcaccg ccgtcgtcgt gctggtggtc gtcgaggtcg
    14761 gaggcaccgt cgaggtggtc gacgtcgtgg tggtcgtcga ggtcggaggc accgtcgagg
    14821 tggtcgaggt cgtcgtggtc ggcggcaccg tcgaggtggt cgaggtcgtg gtggtcgtcg
    14881 aggtcggagg caccgtcgag gtggtcgagg tcgtcgtgg
//
LOCUS       DAOSOA010000036        14905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4042_length_14905_cov_7.330774, whole genome shotgun sequence.
ACCESSION   DAOSOA010000036 DAOSOA010000000
VERSION     DAOSOA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14905
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4042_length_14905_cov_7.330774"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1140
                     /locus_tag="PKY13_04100"
     CDS             160..1140
                     /locus_tag="PKY13_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOT79141.1"
                     /translation="MNLRDLGYLVAVADERHFGRAADLCHVGQPTLSTQIRKLEAELG
                     VDLLERNPRQVLLTDAGERVVERARIILREADNIRELARRAIDPESGTVRLGVFPTLG
                     PYLLPHVVPQVRKRFPKLELLLVEEKTEEVLRRLRNGDLDAGLLALPIHDDQLHVEPL
                     FVEEFLLAVPNDHPLASSVGPIDIGALSGESVLLLEDGHCLRDQALSVCQLAGASERS
                     GFRATSLETLRQMVAAGVGVTLLPELSVRPPVSPSDNITLLRFSEPVPSRTIAMCWRR
                     STAFGEFLPELAEQFRDLPTDLVAPLTGSVPRGEPSRTLSRDETGGGGAS"
     gene            1151..2080
                     /locus_tag="PKY13_04105"
     CDS             1151..2080
                     /locus_tag="PKY13_04105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT79142.1"
                     /translation="MLLRIFTEPQQGASYDDLLAVALVAEETGFDAFFRSDHYLRMGD
                     GSPLPGPTDAWITLAGLARDTTTIRLGTLVSPVTFRLPGPLAISVAQVDQMSGGRVEL
                     GLGSGWFDAEHTAYGIPYPEPIGVRFDLLEEQLAIVSGLWAADEPFSFSGDHYTLVDS
                     PALPKPAQRPGPPIVMGGSGKTRTPRLAAAYAAEWNLPFAPLADFVAGRDRVLAACEA
                     IGRDPGTIVTSAAQVVCVGENDAEIARRAAAIGREVDELRQNGLCGTPAEIVDRLGEW
                     ASAGAERCYLQVLDLADLDHVRLIAEHVLPAVA"
     gene            complement(1982..3022)
                     /locus_tag="PKY13_04110"
     CDS             complement(1982..3022)
                     /locus_tag="PKY13_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT79143.1"
                     /translation="MTRSRTLDHPAAGLVVAVLSAATFGMSGTFARPLLSSGWSPGAA
                     VAVRTAVAGAVLLPAATWSMRGRGRLLIRAWPRLLAFGIVGVAGAQLCYFAAVERLPV
                     GIAILIEFLGPVLLVIATSAAHRAFPPRLTILGAAVAIGGLFLVLDLTGSSDLDPLGI
                     AFALTAAVAVAGYFHLAGHVAADSLPPVALASGGLLTGAAILAVAGITGVLPFDATTA
                     DVDLFGTARAWWVPMAVIVLASTAFAYVTGILAATLLGARVASFVALLEVLFAVVAAW
                     VLLDEVPTIIQGLGGALIVAGVALVRLEGTPADVPDPTSGDGGQDVLGDEPDVVEVGE
                     VEHLQVAPLGPR"
     gene            3128..3685
                     /locus_tag="PKY13_04115"
     CDS             3128..3685
                     /locus_tag="PKY13_04115"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HOT79144.1"
                     /translation="MIFAHDTEASLQAMVDLVNTEARCSHSGEDELANLDHLDAFIER
                     HQYTGSFTRTAGELETVRELRPEFRRFWSADRDESVAIINAMLRQGHALPQLVRHDGW
                     DWHLHATHPDAPFAVRVRVESAMAMIDVVRAGEDERLRRCAADDCDAVFIDFSRNRSK
                     RYCDVGNCGNRMNVTAYRARRAAGA"
     gene            3770..>4511
                     /locus_tag="PKY13_04120"
     CDS             3770..>4511
                     /locus_tag="PKY13_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT79145.1"
                     /translation="MSASLAAGAAGAVAGCASDDAADESDPAGTTTEPTDTTVARAKP
                     ATINDIDHIVILIQENRSFDHYFGTRKGVRGFDDPDVARRPDGRPIWYQDSDHHPEGF
                     VLPYRLDTTTTSGAYGPDPGHAWVLQHAYWNGGKCDSFARLEVANMGYFTAEDIPYPH
                     ALADAFTLCDHSFCSVIGPTTPNRMYSMTGTIDPDGLGGGPVFENFEGPFRWETYPER
                     LQRAGVSWRVYQEEDDYGDNVLEFFANFQ"
     assembly_gap    4512..4611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4612..5307
                     /locus_tag="PKY13_04125"
     CDS             <4612..5307
                     /locus_tag="PKY13_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT79146.1"
                     /translation="QEEDDYGDNVLEFFANFQGLPETDPLFDGGVKVRARDAFAADVA
                     SGNLPQVSWIVAPQATSEHPSAGSPAPGHDLTARTLGALMAAPDVWAKTAFILTYDEN
                     GGYFDHVVPPTPPPGTPGEFTQGTAIGLGFRVPTIIASPWTTGGRVHSGVFDHTSMLR
                     LIETRFGVEVPNLSAWRRETCGDLADVFDFSTTNVEVPDLPDTTAAMAAAEATLSLPP
                     CAPPAVQVEPTVA"
     gene            complement(5372..6166)
                     /locus_tag="PKY13_04130"
     CDS             complement(5372..6166)
                     /locus_tag="PKY13_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOT79147.1"
                     /translation="MTCELGFEIVDRATLALQLAPALSAGPVLEESLVIELDGRALAS
                     DEVAVDHRGRIHMVQSAAGHLDVHYLAVVNTSVALAAPPTALERLSYLRQSRYCPSDR
                     LDGFAADEFGPVAEPYECAARISDWIFERVSYELGSSGPLDTAIETLLGGHGVCRDFA
                     HLCVALCRAVGIPARMASVYSPGLSPMDFHAVAEVAVGDRWLVLDSTRLAPRPTLVRI
                     GTGRDAADTAFCSTVEGYAELTTAMVGASTDTDLPYDDHLTAVAMP"
     gene            complement(6294..7049)
                     /locus_tag="PKY13_04135"
     CDS             complement(6294..7049)
                     /locus_tag="PKY13_04135"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HOT79148.1"
                     /translation="MVFDFLRRRRTDDPPSDAAQRAAGAVSAWSWLDEERRSQLVHDT
                     DTLDRHLRWEAARDFELTDGIRATIAAHAALLTLELGLDCYRNVSSVIVHPTTFERRG
                     EHGIGGGLASDDPVELLGEANHLGPILIAWDTASAEARHPEHGHNVVFHEFAHHLDML
                     DGWVDGTPPLPDDAARSRWIDVCTDAYERLRRGDTGGVLDGYGSVSPGEFFAVATEVF
                     FARPVALQRVRPELYRVLADFYLLDPVAAGATA"
     gene            7163..8152
                     /locus_tag="PKY13_04140"
     CDS             7163..8152
                     /locus_tag="PKY13_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HOT79149.1"
                     /translation="MAGGLVGLLDDIAALAKLAAASIDDVGAAAARASVKSAGVVVDD
                     AAVTPNYVRGLAAERELPIIGRIAKGSLRNKLLFILPAALLLSAISEELVEIVLMCGG
                     TFLCFEGAEKLHHRITHGPEDHAAAELVILDDDHEQKTVAGAIRTDFILSAEIMVIAL
                     KDVLDEGIASRIAILAVVAIVITAVVYGAVAAIVKMDDVGLRLVETGRAGAAKFGRGL
                     VVGMPKVLAVLSTVGIAAMLWVGGHILIVGAYELGWHGPEDLVHRLEDPVHDIAGVGG
                     VLGWLVNTALSAVVGLIVGFLVAAIVTRLPFGKHGTGGKHTDGGDEISTSSAH"
     gene            complement(8184..8441)
                     /locus_tag="PKY13_04145"
     CDS             complement(8184..8441)
                     /locus_tag="PKY13_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79150.1"
                     /translation="MSDIEFDDPETATEELRNAAATPSEEDTPDANVTGFDDDAVSIE
                     RDPADRADADDRHVEAPDDDMSPGVDLTQGAWGSVEPNEPA"
     gene            8440..8916
                     /locus_tag="PKY13_04150"
     CDS             8440..8916
                     /locus_tag="PKY13_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79151.1"
                     /translation="MGDRYPGRAPPTPTASARSPARWRTMRADAHLELVTLVVDDYDA
                     AIEFFVGVLGFDLVEDAPSETNDGRPKRWVVVRPRGAVTGILLARADGAEQSAVVGNQ
                     TASRVGFFLRVDDFDATRSRLDAAGVEFVSPVREMPYGRVTVFVDVAGNRWDLLGE"
     gene            8951..9400
                     /locus_tag="PKY13_04155"
     CDS             8951..9400
                     /locus_tag="PKY13_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79152.1"
                     /translation="MSRLSEIGEKDQWRCWICDEPVDPDMSVNDSRGPSIDSITTKSK
                     AKGKTFGAGTERLAHRGCNTGKGAAAPVVPWADHLFVVDAAPIIGTVERLQRKGGREA
                     MARCPTRADADEAATWLVDRLSRLAPDLAVTTEIEPGGGQFLVVVRA"
     gene            complement(9458..10891)
                     /locus_tag="PKY13_04160"
     CDS             complement(9458..10891)
                     /locus_tag="PKY13_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT79153.1"
                     /translation="MSNTSSPFTTQTATGRRIETDLRRSLIGLQVVLADLDPRAIPLP
                     DVTAVFSLFAEVRRLAGNGETLMAGRVKDARQWAAEGHPSVEDWIAKQTGSTVGAAKD
                     QVTTSERLDQLPTTAERMRAGDVSSEQAGIVADAATLNPAAEDDLLTTAQQDSTKNLK
                     DQAARRKAEARGESANERAKRQHRERRASAWRGSDGTYNFRLEGPVAATAAFNARWER
                     EIDRQYRAAQSTGRREGRDAYAFDAFIALTERGAADPSNGSRPAPRPSPKHLALIRVD
                     LSALVRGTVDNDEICEIAGIGPISVSEARALLGDSILHLVSENGVDVQNITYLGRGPN
                     AVQTLALLWTSQQCSVLGCHRTQTQNDHRIEFAQTKHTRLDELDPLCHFHHGLKTFEG
                     WALVAGTGSRRMVGPDDPDHPGRSGSPVRPRPPDSPGRPHTAVAPDLAERAARAHIAA
                     RRLTPGSMPGGAGAPDPPEQSSLFDTC"
     gene            complement(10983..11375)
                     /locus_tag="PKY13_04165"
     CDS             complement(10983..11375)
                     /locus_tag="PKY13_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79154.1"
                     /translation="MTTELRVTAMDHIVLKCRDIEVTLAWYLGELGLSPVRIDEWRAG
                     DAPFPSVRVSADTIIDLVAVPADQDPKNDRLDHVCLVIEPTDLDALAASGRFDVVGGP
                     GRRYGARGNGTSLYVRDPDGTVVELRHY"
     gene            11495..12076
                     /locus_tag="PKY13_04170"
     CDS             11495..12076
                     /locus_tag="PKY13_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79155.1"
                     /translation="MLFRKAHWDGLADGSITVAFRYQKRPSVRAGGTLQSKAGLLGID
                     SVTAISPSAIDPDSARRAGYPSAAAAVRDLGPRPADKQLYRVEFHLIGEDPRVALRAS
                     HDLDEEELASVLARLARLDRAAPTPWTRPVLQLIADHPGVVSTELAPQVGMERASFKL
                     NVRKLKSSGLTESLEVGYRISPRGLAVLKALGA"
     gene            12069..13322
                     /locus_tag="PKY13_04175"
     CDS             12069..13322
                     /locus_tag="PKY13_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT79156.1"
                     /translation="MRSRRVVARVVAGLVVALVPAGCTDDSTSGDASASSTSAPDTSA
                     TTSTTSTSEPGFDEFTGSIDDFYVVPDPLPVGKPGELIRIQDVDDAGADGGERSVRIM
                     YHSVDAAGRDRATTGTVTVPVDTDEDAGGLPVVTVAPGTVGLASKCALSRTGAPVLDV
                     GVPAVRAMTDYIGMGPAGETMPYLSRESEGHSVLDAARAARQLAGDTSREVAIVGHSQ
                     GGHGAQSAHELASSYAPELDVVGTVSLAPAAMFDRTYGGVDDIVSRIVTSMSLYGLAT
                     EHDDLRFGDYVSAETAEAGQVMATDCVDTVIATLAPLAARPDYWTADPTITEPAATVL
                     KDNDVGHVAADAPLLLVGGTADERVAPQRVQDLFRRLCTTGQATQLIMLDGADHGTEV
                     PRALPQIGRWLTDRFAGVAANDDCS"
     gene            13380..13505
                     /locus_tag="PKY13_04180"
     CDS             13380..13505
                     /locus_tag="PKY13_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79157.1"
                     /translation="MATKQIPSGNSKKVGKTLKEKRAAKHEKQDAKTAARKASGT"
     gene            complement(13516..14166)
                     /locus_tag="PKY13_04185"
     CDS             complement(13516..14166)
                     /locus_tag="PKY13_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT79158.1"
                     /translation="MEIFGRRIATHDELRAHYREPSDLVRRKKFGTLDGQFREFVELS
                     PFCLVATTDALGNTDVSPRGGPPGFVRVVDDRHLLLPDLSGNNLLDSLSNVIDMPSIG
                     LLFLVPGEDETVRVNGRACVTVDPDLLSVWDADLRRPKAAIGIEVIEVFHHCAKAFRR
                     SGMWQPDSWAAPGVAPAFCATYNERLGLEMEPPQLRKMLEQGYATDLAEERPTGSD"
     gene            complement(14222..>14905)
                     /locus_tag="PKY13_04190"
     CDS             complement(14222..>14905)
                     /locus_tag="PKY13_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79159.1"
                     /translation="RSGTSIFGELFEAIPGYTYYSEPFLADVPCPASGPIAVKVPRTA
                     VGSSPPTGASASLDDLRSVVPDPTIFWQVRHPFDAICSLRVGIAQKWAHHPRPPDWQD
                     WIDRPLVERCAHHWATINGAGYALVGGEAVVNRFEDMIRDPVACAVTTCRLVGGDTTA
                     PELVAWADRVQDTNNERFVEAVTSRRHSRPDHDRRVDRWRDNLSPAEVDAVRPIVAAA
                     AATFGYELP"
BASE COUNT         2241 a         5035 c         5295 g         2234 t
ORIGIN      
        1 ggtgactgca ccacggtgcc gtacaagcag atcgtcattg ccatgggcgc cggtgccacc
       61 gccgggctca gcgccttcga ccacctgatc cgcacctcgg ctccggccgc ggcggacgac
      121 acggtgttgg cctcggtctg acgtgacccg cctcacccga tgaatctccg cgacctcggc
      181 tacctcgtgg ccgtcgccga cgagcgccac ttcggtcgcg ccgccgacct ctgccacgtg
      241 ggccagccga cgttgtcgac gcagatccgc aagctcgagg ccgagctcgg ggtcgatctg
      301 ctcgaacgca accctcgcca ggtgctcttg accgacgccg gcgaacgggt ggtcgagcgg
      361 gcgcggatca tcctccggga ggccgacaac attcgtgagc tggcccgtcg ggccatcgac
      421 cccgagtcgg gcaccgtccg gctcggggtc ttcccaacgt tgggcccgta cctcctccct
      481 cacgtggtgc cgcaggtgcg caagcggttc cccaagctcg agctcctgct ggtggaggag
      541 aagaccgagg aagtgctgcg tcgcctgcgc aacggcgacc tcgacgccgg cctgctcgcc
      601 ctcccgatcc acgacgacca gctgcacgtc gagccgttgt tcgtcgagga gttcctgctg
      661 gccgtcccca acgaccaccc gctggcctcg tcggtgggtc cgatcgacat cggcgcgctg
      721 tccggcgaga gtgtgctcct cctcgaggac ggtcactgcc tgcgcgacca ggccctgtcg
      781 gtgtgccagc tggccggagc gagtgagcgg agtgggttcc gggccacgag tctcgagacg
      841 ttgcgccaga tggtcgcggc cggtgttggc gtgacgctcc tgccggagct gtcggtgcgc
      901 cctccggtgt cgccctcgga caacatcaca ctcctgcggt tctccgagcc ggtgcccagc
      961 cggaccatcg ccatgtgttg gcgtcggtcg acggcgttcg gcgagttcct ccccgaactg
     1021 gccgagcagt tccgcgatct cccgaccgac ctcgtcgctc ccctcaccgg ttctgtcccg
     1081 cgaggggagc catcccgcac gctctcgcgg gacgaaacgg gtggcggcgg cgcgtcgtag
     1141 gctgcgcgcc atgctgctcc gcatcttcac cgaaccccag caaggcgcca gctatgacga
     1201 cctccttgcg gtcgcgctgg tcgccgagga gacgggcttc gacgccttct tccggtcgga
     1261 ccactacctg cgcatgggtg acggctcgcc gctgcccggg ccgaccgacg catggatcac
     1321 gctcgccggc ctggcgcgtg acaccaccac catccgcctc ggcacgctgg tctcgcccgt
     1381 gacgttccgg ttgcccggcc cgctcgccat ctcggtggct caggtcgacc agatgtcggg
     1441 cggacgggtc gagctgggcc tgggcagcgg ttggttcgac gccgaacaca ccgcgtacgg
     1501 catcccgtac cccgaaccga tcggcgtccg cttcgacctg ctggaggagc agctcgcgat
     1561 cgtgtccggc ctgtgggccg ccgacgagcc cttctccttc tccggcgacc attacaccct
     1621 cgtcgactcg ccggcgttgc cgaagccggc gcagcgtccc ggcccgccga tcgtcatggg
     1681 cgggagcggc aagacgcgca cgccacggtt ggcagccgcg tacgcggccg agtggaacct
     1741 gccgttcgcc ccgttggccg acttcgtggc cggccgggac cgggtgctcg ccgcgtgcga
     1801 ggcgatcggt cgcgaccccg gcacgatcgt gacgtcggcc gcgcaggtcg tctgcgtcgg
     1861 tgagaacgac gccgagatcg cccgtcgcgc cgccgccatc ggtcgagagg tggacgagct
     1921 ccggcagaac gggttgtgcg gcacgcccgc ggagatcgtc gaccggctgg gcgagtgggc
     1981 ctcagcgggg gccgagcggt gctacctgca agtgctcgac ctcgccgacc tcgaccacgt
     2041 ccggctcatc gccgagcacg tcctgcccgc cgtcgcctga ggtcgggtcc ggaacgtcgg
     2101 ccggtgttcc ctcgaggcgg acgagggcca cgccggcgac gatcagcgca ccgccaaggc
     2161 cctggatgat cgtcggcact tcgtcgagga gcacccacgc ggcgaccacg gcgaacagga
     2221 cctcgagcag cgcgacgaac gacgcgacgc gcgcgcccag cagcgttgcc gccaggatgc
     2281 cggtcacgta ggcgaaggcc gtcgatgcca ggacgatcac ggccatgggc acccaccagg
     2341 cccgcgccgt gccgaacagg tcgacgtcgg cagtggtcgc gtcgaagggg aggacgccgg
     2401 tgatgccggc aacagcgagg atcgccgcgc cggtgaggag gccgcccgac gccaacgcga
     2461 ccggagggag cgagtcggcg gcgacgtggc cggcgaggtg gaagtagccg gcgacggcga
     2521 cggctgcggt gagcgcgaag gcgatgccga gggggtcgag gtcgctcgaa ccggtgaggt
     2581 cgagcacgag gaacagaccg ccgatggcga cggccgctcc gaggatcgtg agtcgcggtg
     2641 ggaacgctcg gtgcgccgcg gacgtggcga tcaccaggag caccgggccg aggaactcga
     2701 tgaggatggc gatgccgacc ggcagccgtt cgacggcggc gaagtagcag agctgcgcgc
     2761 cggcgacgcc gacgatgccg aaggcgagga gccggggcca cgcccggatc aggagtcggc
     2821 cgcggcctcg catcgaccac gtggcggcgg gcaggagcac tgcgccggcc accgcggtgc
     2881 gcacggcgac ggcggcgccg gggctccagc ccgacgacag gagtgggcgg gcgaacgtgc
     2941 cgctcatgcc gaacgtggcc gccgacagca cggccacgac gaggccggcg gcggggtggt
     3001 cgagcgtgcg ggatcgggtc atcgcgtcct tggaccggcg gtgcgggcgg tgctggtgaa
     3061 cgagggtgtc aggagtgtcg taggagtaca ctcgtgacag taggtcgggc cgaagtcagg
     3121 agtcaacgtg atctttgccc atgacaccga agcgtcgctc caggccatgg tcgacctcgt
     3181 gaacaccgag gcgcgttgct cgcactccgg cgaggacgag ctcgcgaacc tcgaccacct
     3241 cgacgcgttc atcgagcgtc accagtacac gggatcgttc acccggacgg cgggcgaact
     3301 ggaaacggtg cgcgaactgc ggcccgagtt ccggcggttc tggtcggcgg accgcgacga
     3361 gtcggtcgcc atcatcaacg ccatgcttcg tcagggccac gcgctgccgc agctcgtccg
     3421 ccacgacggg tgggactggc acctccacgc cacccatccc gacgccccgt tcgcagtgcg
     3481 cgtccgagtc gagtcggcga tggcgatgat cgacgtcgtc cgggccggcg aggacgaacg
     3541 cttgcgccgg tgcgccgccg acgactgcga cgccgtcttc atcgacttct cccgcaaccg
     3601 gtcgaagcgc tactgcgacg tcggcaactg cggcaaccgc atgaacgtga cggcctaccg
     3661 ggcgcggcgc gccgcgggcg cgtaacgtcg ttccccgttc accgggagtt cacccgggac
     3721 gctggaccga gggggttcgt cagtggggac gtggtcgagg cgcgagttct tgtcggcgtc
     3781 gttggcggcg ggtgcggcgg gcgccgtggc agggtgcgcg tccgacgacg ccgccgacga
     3841 gtccgacccc gcgggcacga ccaccgagcc cacggacacg accgtcgctc gggcgaagcc
     3901 ggcgacgatc aacgacatcg atcacatcgt gatcctcatc caggagaacc gctcgttcga
     3961 ccactacttc ggcacccgca agggggtgcg cgggttcgac gatcccgacg tggcgcggcg
     4021 tcccgacggt cgaccgatct ggtatcagga cagcgaccat catcccgagg gattcgtcct
     4081 gccctaccgc ctcgacacca cgacgacgtc gggggcgtac gggccggatc cggggcatgc
     4141 ctgggtgctg caacacgcgt actggaacgg tgggaagtgc gactcgttcg cccgactcga
     4201 ggtcgccaac atgggctact tcacggccga ggacattccc tacccccacg cactcgccga
     4261 cgccttcacg ctctgcgacc actcgttctg ttcggtgatc ggccctacca cgcccaaccg
     4321 catgtactcg atgaccggca ccatcgaccc cgacgggctc ggcggcggac cggtgttcga
     4381 gaacttcgag ggcccgttcc gctgggagac ctacccggag cgactgcagc gcgccggggt
     4441 gtcgtggcgg gtgtatcagg aggaggacga ctacggcgac aacgtgctcg agttcttcgc
     4501 caacttccaa gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaggaggag
     4621 gacgactacg gcgacaacgt gctcgagttc ttcgccaact tccaaggtct gcccgagacc
     4681 gacccgctct tcgacggtgg ggtgaaggtt cgggcccggg acgccttcgc agcggacgtg
     4741 gcgtcgggca acctgccaca ggtgtcgtgg atcgtggcgc cgcaagcgac ctcggaacat
     4801 ccgtcggccg ggtcaccggc accgggacac gatctcacgg cgcgcacgct cggggcgctc
     4861 atggcggcgc ccgacgtgtg ggcgaagacc gcgttcatcc tcacctacga cgagaacggt
     4921 gggtacttcg accacgtcgt tccgccgacg ccgcctccgg gcacccccgg cgagttcacg
     4981 cagggaacgg cgattgggct cggattccgg gtgccgacca tcatcgcctc gccctggacg
     5041 acgggcggac gggtgcacag cggggtgttc gaccacacgt cgatgctccg cctcatcgag
     5101 acgcggttcg gagtcgaggt tcccaacctc agtgcgtggc gccgcgagac gtgcggtgac
     5161 ctcgcagacg tcttcgactt ctcgacgacg aacgtcgagg tgccggacct gcccgacacg
     5221 acggcggcga tggccgctgc cgaggcgacg ctgtcgctgc ctccgtgcgc accgccggcg
     5281 gtccaggtcg agccgaccgt cgcgtagcgc gatccgggct gcggaggacg cagcacgtca
     5341 cctccagcac ggacggtgat gagaccgact cctacggcat ggcgaccgcg gtcaagtggt
     5401 cgtcataggg cagatcggtg tcggtcgacg cgccgaccat cgcggtcgtc agttcggcgt
     5461 agccctccac ggtgctgcag aaggccgtgt cggctgcgtc gcggccggta ccgatgcgca
     5521 cgagcgtcgg tcggggagcg agccgggtgc tgtcgagcac cagccagcgg tcgccgaccg
     5581 cgacctcggc cacggcgtgg aagtccatcg gggacaggcc gggggagtac accgacgcca
     5641 tgcgcgccgg gataccgacg gcgcgacaca acgcgacgca caggtgggcg aagtcgcggc
     5701 acacgccgtg gccaccgagc agtgtctcga tggccgtgtc gaggggaccg ctgctcccga
     5761 gctcgtagga cacccgctcg aagatccagt cggagatgcg cgccgcacac tcgtacggct
     5821 cggcaaccgg tccgaactcg tcggcggcga acccgtcgag acggtccgac gggcagtagc
     5881 ggctctgccg gaggtaggag agccgttcga gcgccgtcgg cggggcagcg agcgcgaccg
     5941 atgtgttcac cacggcgaga tagtgcacgt cgaggtggcc ggcggcgctc tgcaccatgt
     6001 ggatgcggcc ccgatgatcg accgcgacct cgtcggaagc gagggcgcgc ccgtcgagtt
     6061 cgatcacgag tgactcctcg aggaccggtc cggcgctcag cgccggggcg agctgcaacg
     6121 cgagcgtcgc acgatcgacg atctcgaagc cgagctcgca ggtcacggtg gtcagcgttc
     6181 tggttggggg gtcgacgtgg tgagggatgg cacgaccctg ccagtcccgg accccgacga
     6241 caacggagcg gacctcctgt tcacgcactc gtcacattgc gatcccccga cgatcaggcg
     6301 gtggcgccgg ctgccaccgg gtcgagcagg tagaagtcgg ccaggacccg gtagagctcg
     6361 gggcggacgc gctgcagggc gaccggtcga gcgaagaaca cctcggtcgc gacggcgaag
     6421 aactcgccgg ggctcaccga cccgtagccg tcgagcaccc cgccggtgtc gccgcggcgg
     6481 agacgctcgt acgcgtcggt gcacacatcg atccaacggg agcgcgccgc atcgtcgggg
     6541 agcggcggcg tgccgtccac ccaaccgtcg agcatgtcga ggtgatgggc gaactcgtgg
     6601 aacaccacgt tgtgcccgtg ctctgggtgc cgagcctcgg cggaggccgt gtcccacgcg
     6661 atcaggatgg ggccgaggtg gttcgcctcg ccgaggagtt cgacggggtc gtcggatgca
     6721 agtcctccgc cgataccgtg ctcgccccgg cgctcgaacg tggtcgggtg cacgatcacc
     6781 gagctgacgt tgcggtagca gtcgagcccg agctcgaggg tcaacagcgc ggcgtgcgca
     6841 gcgatcgtcg ccctgattcc gtcggtgagc tcgaagtcgc gggccgcttc ccaccggagg
     6901 tgtcgatcga gcgtgtcggt gtcgtggacc agctgcgacc tccgttcctc gtcgagccac
     6961 gaccacgccg agacggcgcc ggcggcacgc tgagcggcat ccgacggcgg gtcgtcggtg
     7021 cgccggcgac ggaggaagtc gaagaccatc gggacatcat ggtcggccgg ccgaccggcg
     7081 ctgcagcaac tccgggatcg ccgccgccgg cggccggccg gtcgttacga tgcaggctcc
     7141 ccgaggtcga tggagcaacg agatggcagg tggcctggtc ggcctgttgg acgacattgc
     7201 agcactggcg aagctggctg ccgcgtcgat tgacgacgtg ggtgccgctg cggcacgagc
     7261 gagcgtgaag tcggcggggg tcgtggtcga tgatgcggcc gtcaccccga actacgtgcg
     7321 cggcttggcc gcggagcggg aactcccgat catcggcagg atcgccaagg gttcgctgcg
     7381 caacaagctg ctgttcatcc tcccggcggc cctgttgctg agcgccatct ccgaggagct
     7441 ggtcgagatc gtcctcatgt gcggcggtac gttcctctgc ttcgaaggcg ccgagaagct
     7501 gcaccaccga atcacccacg ggcccgagga tcacgccgcg gccgagctcg tgatcctcga
     7561 cgacgatcac gagcagaaga cggtggccgg ggcgatccga acggacttca tcctgtcggc
     7621 ggagatcatg gtcatcgcgc tcaaggacgt cctcgacgaa gggatcgcat cgcggatcgc
     7681 gatcctcgcc gtcgttgcca tcgtgatcac cgcggtcgtg tacggcgccg tggccgccat
     7741 cgtgaagatg gacgacgtcg gactccggtt ggtcgagacg ggacgagcgg gcgctgcgaa
     7801 gttcgggcgc ggcctggtcg tcggcatgcc caaggtgctc gccgtgctgt ccaccgtggg
     7861 catcgccgcg atgctgtggg tgggcggcca catcctgatc gtcggcgcgt acgagctggg
     7921 atggcacggt cccgaagacc tcgtccatcg cctcgaggat cccgtgcacg acattgccgg
     7981 ggtcggcggg gtgctcggat ggctggtcaa caccgcgctc tccgcagtgg tcggcctcat
     8041 cgtcgggttc ctggtcgccg cgatcgtgac ccgcctgccc ttcggcaagc acggcacggg
     8101 cgggaaacac accgacggtg gcgatgagat cagcacgtcg tcggcgcact gacctgacac
     8161 cgtcccggcc tgtcgccgac gggtcaggcg ggctcgttgg gttcgaccga accccacgcg
     8221 ccctgggtga ggtcgacgcc gggcgacatg tcgtcgtcgg gagcctcgac gtggcggtcg
     8281 tcggcgtcgg cgcggtcggc cgggtctcgc tcgatcgaca cggcgtcgtc gtcgaagccg
     8341 gtgacgttgg cgtcgggcgt gtcctcctcc gacggcgttg ccgccgcgtt ccgcagttcc
     8401 tcggtcgccg tctcgggatc gtcgaattcg atgtcgctca tgggagaccg gtaccccggc
     8461 cgggcaccgc cgacaccgac cgcgtcggcg cggtcccccg cccgatggcg caccatgcgt
     8521 gctgacgccc atctcgaact cgtcacgctc gtcgtcgacg actacgacgc cgccatcgag
     8581 ttcttcgtcg gcgtcctcgg cttcgatctg gtcgaggacg ctccttccga gacgaacgac
     8641 ggccgcccca agcggtgggt cgtggttcgc ccgcgagggg ccgtaaccgg catcctgctg
     8701 gcccgggccg atggcgcgga gcaatcagcg gtcgtcggca accagacggc cagccgcgtc
     8761 gggttcttcc tccgggtcga cgacttcgac gccacgcgct cgcgtctcga cgctgcgggc
     8821 gtcgagttcg tctcccccgt tcgtgagatg ccgtacggac gcgtcaccgt gttcgtcgac
     8881 gtcgccggca accggtggga tctgctcggg gagtgagggc cgctgcgggc cgggccggta
     8941 cggtgcggac atgagtcggc tgagcgaaat cggtgagaag gatcagtggc ggtgctggat
     9001 ctgtgacgaa ccggtcgacc cggacatgtc ggtcaacgac tcgcggggcc cgagcatcga
     9061 cagcatcacg accaagtcga aggcgaaggg gaagacgttc ggcgccggca cggaacgcct
     9121 cgcccatcgt ggctgtaaca cgggcaaggg ggctgctgcc cccgtcgtgc cctgggccga
     9181 ccacctgttc gtcgtcgacg cggcgccgat catcggcacc gtcgaacggc tgcagcgaaa
     9241 gggtggccgg gaggcgatgg cgcggtgtcc gacccgagcc gacgcggacg aggccgcgac
     9301 ctggctcgtc gaccgcctct cgcgactggc gcccgacctg gccgtcacga ccgagatcga
     9361 acccggcgga ggacagttcc tcgtggtggt gcgcgcctga gcgtcattgg gcgcgggtcg
     9421 gggtgggtag gaaccggaga cgttcagccc gggatgctca gcacgtgtcg aacaggctgg
     9481 actgttcggg tgggtctggt gcccccgcgc ctccgggcat cgagccgggg gtgaggcgcc
     9541 gtgcggcgat gtgggcgcgc gcggctcgtt cggcgaggtc gggagcgacg gcggtatggg
     9601 gacggccggg agagtcggga gggcgtggac ggactggaga gccggaccgg cccgggtgat
     9661 cgggatcgtc gggaccgacc atccgtcgac tgcccgtgcc ggcgacgagg gcccagccct
     9721 cgaaggtctt gagaccgtgg tggaagtggc acagcggatc gagctcgtcg aggcgggtgt
     9781 gcttggtctg ggcgaactcg atgcggtgat cgttctgggt ctgcgtgcgg tgacacccca
     9841 gcaccgagca ctgctgggag gtccacagaa gagcgagcgt ctgcacggcg ttggggccgc
     9901 gaccgaggta ggtgatgttc tggacatcga ccccgttctc cgacacgaga tgcaggatcg
     9961 aatcgcccag caaggcccgc gcctcactga ccgagatggg gccgatgccg gcgatctcgc
    10021 agatctcgtc gttgtcgacg gtgccacgga cgagcgccga caggtcgact cggatgagcg
    10081 cgaggtgctt gggactcggc cgtggcgccg gcctggaccc gttgctcggg tcggcggcgc
    10141 cgcgctcggt cagggcaatg aacgcgtcga acgcgtaagc gtcgcggcct tcgcgacgcc
    10201 ctgtcgactg ggcggcgcgg tactggcggt cgatctcgcg ttcccagcgg gcgttgaacg
    10261 ccgcggtcgc ggcgacaggc ccctcgagtc ggaagttgta cgtgccgtcg ctcccgcgcc
    10321 acgcggatgc ccgtcgttcg cggtgctgtc gcttcgctcg ttcgttggct gattcccctc
    10381 gggcttcggc cttgcgtcgg gcggcctggt ccttcaggtt cttggtcgaa tcctgttgcg
    10441 cggtggtgag caggtcgtcc tcggcggcgg ggttgagggt ggcggcatcg gcgacgatgc
    10501 cggcctgctc cgacgagacg tcacctgccc gcatcctctc cgccgtggtg gggagttggt
    10561 cgagacgttc cgacgtcgtc acctggtcct tcgcggcgcc gacggtggag cccgtctgct
    10621 tggcgatcca gtcctcgacc gacgggtgac cctcagcggc ccactgccgg gcatccttga
    10681 cccgaccggc catcaaggtc tcgccgttgc cggccaaacg ccggacctcg gcgaacagac
    10741 tgaacaccgc ggtcacgtcc ggcaacggga tcgcacgagg gtcgaggtcg gccagcacca
    10801 cctgcaggcc gatcagcgag cggcgaagat cggtctcgat tcggcggccc gtcgccgtct
    10861 gggtggtgaa gggcgaactg gtgttcgaca tgcctacatt ctgtacgcga cccctgacac
    10921 tgaagcccga tcctgacgtc atcctgacga ttgggcgact tcacccagtc gtcgggagcg
    10981 gctcagtagt ggcggagctc gacgacggtg ccgtcagggt cgcgtacgta gagcgacgtg
    11041 ccgttgccgc gggcgccgta gcgcctgcca ggcccgccga cgacatcgaa tcggccgctc
    11101 gctgcgagcg cgtcgagatc ggtcggttcg atcaccaggc agacatggtc gagccggtcg
    11161 ttcttcgggt cctgatcggc cggcacggcc acgaggtcga tgatcgtgtc ggcgctgacg
    11221 cggaccgacg gaaacggggc gtcgcccgcc cgccattcgt cgatgcgaac ggggctgagt
    11281 ccgagttcgc cgaggtacca ggcgagcgtc acctcgatgt cacggcactt cagcacgatg
    11341 tggtccatcg cggtcacccg tagctcggtt gtcatcgtcg acctcctcgt cctggtcggg
    11401 ccagcctgcc agagcgaccg agtgtcgagc atctgctact cgggaccggc gatcgggaca
    11461 gtgaatcgac ccgggagatg gctagggtcg ccgcatgttg ttccggaagg cgcactggga
    11521 cggcctcgcc gatggatcga tcacggtggc gttccgctac cagaagcgac cgtcggtgcg
    11581 ggcgggtggc acgctgcagt cgaaggccgg actcctcggc atcgattcgg tcacggccat
    11641 cagcccatcc gcgatcgacc cggactccgc tcgccgggcc gggtacccat cggccgcggc
    11701 ggcggtgcgc gacctcggcc cgcggccggc ggacaagcag ctctaccggg tcgagttcca
    11761 cctcatcggt gaggaccccc gcgtggcgct gcgtgcgagc cacgacctcg atgaggagga
    11821 gctggcgagc gtgctggcac gcctcgcccg tctcgaccgc gccgctccga ccccgtggac
    11881 ccggccggtg ctccagctga tcgccgacca ccccggcgtc gtgtccaccg aactggcacc
    11941 gcaggtcggc atggaacggg catcgttcaa gctcaacgtc cgcaagctca agtcgtcggg
    12001 gctgaccgag agcctggagg tggggtaccg catctcgccc cgtggactcg ccgtcctgaa
    12061 ggcgctcggt gcgtagccgc cgggtcgtcg cccgcgtcgt cgccggtctc gtcgtcgcgc
    12121 tggtgccggc ggggtgcacc gacgactcca cctccggcga cgcgtcggcc tcgtcgacga
    12181 gcgctcccga cacgtcggcg acgacgtcca ccacctcgac ctccgagccc gggttcgacg
    12241 agttcacggg gtcgatcgac gacttctacg tcgttcccga cccacttccg gtggggaagc
    12301 ccggcgaact catccgcatt caggacgtcg acgacgccgg cgccgacggc ggcgaacgct
    12361 cggtccggat catgtaccac tccgtcgatg ccgccggccg tgaccgtgcg acgacgggca
    12421 cggtgaccgt tcccgtcgac acggacgagg acgccggtgg cctgccggtc gtcaccgtcg
    12481 ctcccggaac cgtcgggctc gcgtccaagt gcgcgctcag ccgcaccggc gcgccggtcc
    12541 tcgacgtcgg cgttcccgcc gtccgcgcca tgaccgacta catcggcatg ggcccagcgg
    12601 gtgagaccat gccctatctg tcacgggaga gcgaagggca cagcgtgctc gacgcggccc
    12661 gcgccgcccg gcagctcgcc ggcgacacgt cacgcgaggt cgcgatcgtc ggccactccc
    12721 agggcggcca cggtgcgcag tcggcacacg aactcgcttc ctcgtacgcc cccgagctcg
    12781 atgtcgtcgg taccgtgtcg ttggctccgg cggcgatgtt cgaccgcacg tatggcggcg
    12841 tcgacgacat cgtgtcgagg atcgtcacgt cgatgagcct gtacggcctc gccaccgagc
    12901 acgacgacct ccggttcggc gactacgtca gcgcagagac tgcggaggcc ggtcaggtca
    12961 tggcgaccga ctgcgtcgac acggtcatcg cgacgctcgc accgctcgcc gcccggcccg
    13021 actactggac ggccgacccg acgatcaccg agcccgccgc caccgtgttg aaggacaacg
    13081 acgtcggaca cgtcgcggct gacgctccac tcctgctcgt cggcggcacg gcggacgagc
    13141 gggtcgcccc acaacgggtg caggacctgt tccgccggtt gtgcaccact ggccaggcca
    13201 cgcagctgat catgctcgac ggagccgacc acggcaccga ggtcccgaga gcgttgccac
    13261 agatcggtcg atggttgacc gaccgattcg ccggcgtggc ggcgaacgac gactgctcct
    13321 gacacgccgg tctccgtcga ccgccgtacc ggccctcgac atcggtcgta gggtggctca
    13381 tggctaccaa acagatcccg tcgggaaatt ccaagaaggt cggcaagacg ctgaaggaga
    13441 agcgggcggc gaagcacgag aagcaggacg ccaagacggc ggcccgcaag gcgtcgggca
    13501 catagcccgc tcgcctcagt cgctcccggt cggtcgttcc tcggcgaggt cggtcgcgta
    13561 gccctgctcg agcatcttgc gcagctgcgg tggctccatt tcgagcccga gccgctcgtt
    13621 gtaggtggcg cagaaggcgg gggcgacgcc gggcgcggcc catgagtcgg gctgccacat
    13681 tccacttcgc cggaacgcct tggcgcagtg gtgaaacacc tcgatcacct cgatgccgat
    13741 ggccgccttc ggtcgtcgga ggtcggcgtc ccacaccgac aggagatcag gatcgacggt
    13801 gacgcaggcc cgtccgttga cgcgaaccgt ttcgtcctct cccggcacca ggaacaacag
    13861 gccgatcgac ggcatgtcga tcacgttgct cagcgaatcg aggaggttgt tgcccgacag
    13921 gtcggggagc aacaggtgtc ggtcgtcgac cacgcggacg aaccccggcg gcccgccccg
    13981 gggcgacacg tcggtgttgc cgagggcgtc ggtcgtggcg accaggcaga acggcgagag
    14041 ctcgacgaac tcccggaact ggccgtccag cgtcccgaac ttcttccgac gaacgaggtc
    14101 gctgggctct cgatagtggg ctcgcaactc gtcgtgggtc gcgatccgac gtccgaagat
    14161 ctccatcggg ccagggtagg gcggtcagat cgagcgaagc gggagccgcc gtgttggtgg
    14221 ttcagggcag ctcgtacccg aaggtggcgg cggccgccgc gacgatcggc ctgacggcgt
    14281 cgacctcagc gggcgacaga ttgtcccgcc accggtcgac gcgccggtcg tggtcgggcc
    14341 gggagtgacg gcgggacgtg accgcttcga cgaaccgctc gttgttcgtg tcctgcacgc
    14401 ggtcggccca tgcaacgagc tccggcgcag ttgtgtcgcc gccgacgagt cggcaggtgg
    14461 tcacggcgca cgcgacgggg tcgcggatca tgtcctcgaa tcggttgacc acggcttcac
    14521 cccctaccag cgcgtacccc gccccgttga tcgtcgccca gtggtgggcg catcgctcga
    14581 cgagcgggcg gtcgatccag tcctgccagt cgggcgggcg gggatgatgg gcccacttct
    14641 gcgcgatccc gacccgaagc gagcagatcg cgtcgaacgg gtggcggacc tgccagaaga
    14701 tcgtcgggtc cggtaccacg gaccgcaggt cgtcgagaga agccgacgct cccgtcgggg
    14761 gcgatgagcc aacggcggtg cgagggacct tgaccgcgat cgggcccgag gcggggcacg
    14821 gcacatcggc gaggaagggc tcggagtagt aggtgtagcc cggaatggcc tcgaacagtt
    14881 cgccgaagat cgacgtgccg ctccg
//
LOCUS       DAOSOA010000037        14719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4109_length_14719_cov_6.543031, whole genome shotgun sequence.
ACCESSION   DAOSOA010000037 DAOSOA010000000
VERSION     DAOSOA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14719
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_14719_cov_6.543031"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PKY13_04195"
     CDS             <1..1359
                     /locus_tag="PKY13_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT79160.1"
                     /translation="YYPGATVEGRALGAVRVKLGDAPSSELAAGAGLISGSVDGGPTV
                     TLAGPGQRILLKMSGTTLQYQRVDNGAGPVTTLTSGGGRYTITWDAGSNLAVSATSRS
                     YNAGQLRVIPGSGTFALVLGGLSMQQYLYGLGEMPASWSIEALKAQAIAGRSFAAYRL
                     AHPQGADFDLYASVSDQAYVGITQTSGASGANWIAAVDGTDGQVLTSGGQPIQAFYSS
                     SNGGHTERSDYVFVATLPYLVAAADPYDQATGNANFSWTRTYSDAELGAWLNAAGRGV
                     GTVTGVSIGGNVGASGRVDKATVTVSGTDRTVTMTGNQFRLAVNVGAPSSRDLLSTKF
                     ALGDGPAAAAPPAPAPTDTTAPDLKVLVGSPMRFGSSGNICAFVMSDEVAITAIHIRL
                     GRTDLKSRVGGVGPTFPQLMCINIPPKLRPRAATKVVLTGAALDVAVNMRFVQQTVTI
                     AR"
     gene            complement(1343..1912)
                     /locus_tag="PKY13_04200"
     CDS             complement(1343..1912)
                     /locus_tag="PKY13_04200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOT79161.1"
                     /translation="MLILASGSPRRLQLMADAGYEFTVEPADIDETPLPNEPAATYVA
                     RLATDKARHVAERHPEAVVIGADTIVALDGELLGKPADADDAGAMLGRLSGRAHEVLT
                     GVAVARDGDVYGIVEVSRVWFRTLGRPEIDAYVATGEPLDKAGSYAIQGLGGAFVERT
                     EGTWDNIVGLPMAVVARLLAERGGLSGRS"
     gene            complement(1922..3037)
                     /gene="dusB"
                     /locus_tag="PKY13_04205"
     CDS             complement(1922..3037)
                     /gene="dusB"
                     /locus_tag="PKY13_04205"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT79162.1"
                     /translation="MRIGPITLDVPVVLAPMAGVTNAPFRRLCRAEGAGLYVSEMITA
                     RAYVEGNAKTQRLAHFDDDEPLRSIQLYGIDAFHVGEAVRRLVDSDHVDHIDLNFGCP
                     APKVTKNGGGSALPVRKRLLRDIIAAAVAGAGDVPITAKFRMGVDDDHPLTYLDTGRI
                     AEDEGCAMIALHARTAEQLYSGAARWEAIGRLKEHVTSIPVLGNGDIWEATDAVAMMA
                     ATGCDGVVVGRGCLGRPWLFRDLVAAFDGREIPPPPTLGEVCDTMLRHIDLLQAWAGE
                     WLGVREFRKHTGWYLKGYPVGSELRRELNQLQSRDQLAESLGRLDRSLTMTPEGPRAK
                     RGHQNGPRAVSLPEHWFELADSDEALDAAAESITSGG"
     gene            complement(3131..4309)
                     /locus_tag="PKY13_04210"
     CDS             complement(3131..4309)
                     /locus_tag="PKY13_04210"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOT79163.1"
                     /translation="MRMLRNPRVVAVPDPTAPTTARPHDLPPGALPTPTALHRLIDGY
                     SPSALVDVPSLARRTGLGRVLVKHEAQRFGLPAFKIVGASWATYRLLAARYAAAHGTA
                     PAATTFDEFRRAFAEFTDLTLVTATDGNHGRAVARAAAWFGLGADIYMPAGTVAARID
                     AIESEGARVTVVDGDYDLAVETAAATAGPDRLVVSDTSWPGYTEVPTWIAEGYATIFG
                     ELDAQLAALDVTLDGIVIPVGVGAFFQAALDHWPAAPPVGPCRLAVEPLAADCLFRSI
                     QAGQPVTVPGPHDSMMAGMNCGTVSMIAWPALRDRTDWAIGIEDTYAADAMRLLASDG
                     IVVGETGAGTVGGLLAIAAESPESFVEMGLGQDSTVVCICTEGATDPVNYERVVGTAP
                     "
     gene            4393..5916
                     /locus_tag="PKY13_04215"
     CDS             4393..5916
                     /locus_tag="PKY13_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HOT79164.1"
                     /translation="MSAPTSAPSKLGLEREIVDQAVLERAISRFRDEGIVLPTFAQLA
                     DPSTIPAEIVERLAGIDPRVGDPANLFRVHWHNTAAADGSFSATADLPDHVVLGPELT
                     GVEAPIIVLFGDRFPMIDAHKVLAAYACLVARLVTGQFDPTEHRAVWPSTGNYARGGV
                     AISRIMASRGVAVLPENMSRERFEWLERWVVEPGDIIRTPGSESNVKEIYDACAELEA
                     DPTNFILNQFCEFANYLVHRHVTGAALERVYRSVAAGDGGPSQLAAWVSATGSAGTIA
                     AGDHLKEVFGTKIVAVEALECPTMLHNGFGEHNIQGIGDKHIPLIHNVTNTDVVVGIS
                     DQATDELGLAFVSESGRELLRRRGVDEATIASLEHMGFSSICNVLAAIKTAKVLGLGA
                     DQAVITVATDGSMLYASEHEALLAERFSGEFGDAQAAAVVARHLDGADVANVEELDTA
                     GRNRIFNLGYFTWVEQQGIDLETFEARRHQSFWDDLSSYVPRWDELIGEFNAATGLA"
     gene            5924..7174
                     /locus_tag="PKY13_04220"
     CDS             5924..7174
                     /locus_tag="PKY13_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HOT79165.1"
                     /translation="MLLTCRGCGATVDPLGLSPWACPRRDAAGLTDVDHVLVLSRESV
                     QSVALPRDRTGGAGVNPFVRYRERLGSYAAAIEAGWSDAQFVDTVGSLDDAVVAVWGS
                     GFTVTPTTSQPTLSTAVGVDGLWVKDETGNVSGSHKARHLFGLALWLAVRSPADDDAR
                     LAIASCGNAALGAGVIAAAMRRPLDVYVPVWADESVVARLVGLGANILRCERREGEVG
                     DPCVLRMREAVAGGSLPFGCQGPDNGLTIDGGRTIGWELAEQAPGLVAVYVQMGGGAL
                     ASSLARGLAEGGSDARVIAVQTEGCAPFDRAWRRVEQVGLDEAVHHRSAAMWPWESEP
                     TSAATGILDDETYDWAGVAAGLELSHGTSVVAPESAVVRAHALAHEHTTIDADPTGTA
                     GLAGVLTAATAGAVGVLFTGLTRR"
     gene            complement(7175..8191)
                     /locus_tag="PKY13_04225"
     CDS             complement(7175..8191)
                     /locus_tag="PKY13_04225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT79166.1"
                     /translation="MAAEATTQPVAIYLQDAHTIREGMDFARYAESKGFDAVWQAESR
                     LVRESTVPMAAFASVTDRIKVGSGVADVWSRNPARLAATFSTLDDLAPGRVILGLGAW
                     WDPLAAAVGITRSKPLGVMREVVTAVRALLNNETVTMHGDYVHLDGIELDYVYQERRP
                     KDVPIYIGATGMKMMELTGEIADGVVLNYLVSPDYNATAMEHLTIGAERAGRSIDDID
                     RPQLVVCSVHEDRQTALDMARMMVTQYLGQQPHIMKASGVPQSLLDSVGEVLTWPATH
                     EQVEAASKLVPDEIVQMLTASGTPAEAKARVADYIAGGATCPILYPLGDVKAMIDTFS
                     GWTL"
     gene            complement(8296..9453)
                     /locus_tag="PKY13_04230"
     CDS             complement(8296..9453)
                     /locus_tag="PKY13_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79167.1"
                     /translation="MTLTTRRATAIGALLAVTLVLSACQNLPRRTAGAKCKTVGEHAQ
                     DGTWVLKCGSNKRWNKLMTIAAANDAVANWLRSQVPPTPPAPPAPPVTAPPAAPALTS
                     IGATNRAQIAESPGLVSPGLYSTTVPTGSACIIEVFGGPSRRVRGSSGGPLYMNLPAG
                     MSVSTAGPCVWNKGDLPPVTMPATGDGMYRTGAEIQLGLYTAPGGSDCYWETSDNADG
                     SLEVINDIYYGPGPQLAIIEAGDAYFTSDGCGPWTPLTALPAQYLQVASAIDNYPLQG
                     LRAFYPGSQFTVTATPGVTTFTTPQFTLQIAPPDSGPFTAGDFTVSRYNFTAPGNVLV
                     NVTSPGRGCNVIPGTATISDVVLDVDGSASSMHAIVFAECDGKRFAVNVQY"
     gene            9571..10710
                     /locus_tag="PKY13_04235"
     CDS             9571..10710
                     /locus_tag="PKY13_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79168.1"
                     /translation="MDEDRPKLRLVAPDERASEADEAPPESSTPPPTPPLPPSSGTDD
                     DRHRLAWMSAAIPLCIGIAILVVGLAVALRLLMDTGGSDDANGDSPAITRPAGPTSSI
                     DPLSLLPRVPTSVGETTTLPAPTVPTTAAPTTVPRNVDPGLIALPVPGGAPAAPASPR
                     PPSPTAGSASVRIFNGFSAGETLDVWEISGPTPVRYGSLPYGGLVEMVAGGRLLPSGD
                     ALQLRFVRPGGDPTGTIQDGTSSKTGPWGRNFTPADGSSVTLVLSRNPGLRVVDIDNR
                     RALAAVRSGQVHVVPVVYHLVLEGTRAKQWAGDGLGCLGVLTTDTDAELDVDVGTALR
                     LALVDDPTCSRTVTDALVLGESTAVAVVGLEIPGDRAQLIAVPLG"
     gene            10741..11877
                     /locus_tag="PKY13_04240"
     CDS             10741..11877
                     /locus_tag="PKY13_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79169.1"
                     /translation="MRRKIFLPMLVLAIAAVVPACDFAKVGARCSASQGAARNNTHVL
                     FCVKGKWKATVTIGQAAEIVMSSWPGSVTGGPAEVRATSGGAFPSMTFFVKTRGGQPA
                     ANVDVKLTATSGPGFSGTSFTVRTNGAGAVNTTPGQSGMPNVTMGSGVGDVKVDINAG
                     PLPTPIGSFVIKVSGGAPSVVAAVSGRDQQITAGGTFQPWVFEVRDSKGAPTIPSTVK
                     VNSGGLSGVSYTQSGATITVTAPPQYVAANQQSLTVEVSGAEPFKMTTFATGYTNWTI
                     VAGAPDDADIYGDDQSAPVNTRFANSLVVDVIDAYGNSVTGLPITYTSIDGGSGASGT
                     TMPNFYFNPISAVFVDANGTAGAWTVRADIPGLGTYDFSLTNTP"
     gene            complement(11884..12933)
                     /locus_tag="PKY13_04245"
     CDS             complement(11884..12933)
                     /locus_tag="PKY13_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT79170.1"
                     /translation="MTVSVTSALQNVTPGLVCAHHHLYSALARGMPAPPETPTSFLGV
                     LEQIWWRLDTALDLEMIRASARLGALEALECGTTALIDHHESPNAIEGSLDVIAEACA
                     EVGVRCDLAYGVTDRHGADGAARGLAENERYLRAGGRGRVGVHAAFTCTDDTIAAAAG
                     LAEDLGVGLHIHVAEGPDDVDAAARLAELSRDDWLIIHGVHLPDDHGVRGTLVHNPRS
                     NMNNAVGYARPTRFANPVALGTDGIGADMLDEFRVAYVRLRESDVTETPSTAWSWIET
                     GWALFPEARDDTVTWSYDGADDPWRLAFTTGVHAIDVDIDGEPALRGGRATRVDPDEI
                     RAHAAEQATRLFSRL"
     gene            complement(12930..>14719)
                     /locus_tag="PKY13_04250"
     CDS             complement(12930..>14719)
                     /locus_tag="PKY13_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT79171.1"
                     /translation="VLTPVVDPVAALADGADIAVWGTDTNELSRSTYARGDVDSALAQ
                     SAHVAHETFQTQRIEHAFLEPESTLAVPSYRPGTESPGDMGVDQRTLYVYSGGQGVWD
                     DRNQIAAMLGVERDQVTVELVSNGGAFGGKEDMSNQGHTALAAWLLDRPVKCTLSREE
                     SFLMHAKRHPIGMEYWAGCDEDGRLTGLRVRMIGDSGPYASVGMKVLERAAGHASGPY
                     RVPSIDVDSVAVRTNNPVCGAFRGFGANQAQFAMEGVMDRLAAKVGISGWEIRKRNVI
                     RPGEVWGPGQVMDDGSAGAEVCLDEVKDRYDAAVADGKIVGLGLGLKNSGLGNGFREV
                     SGAVIRFRSSDGRVEVRHSWTEMGQGVHTVALQVAAEELGIDPNLIDVIVDTTRELGF
                     GQTTGSRGTLMAAGAVSAACRVADEGGRQPDVDYHAEHIVDWTQKLGDPENPNPTIHA
                     AFGYATQLVIMDRDTGTIEEVVAIHDVGRAMNPLLCEGQIEGSVHMGLGYALSEDFPS
                     DERGFPTNMTLRSLGILRPKDIPTITVQLVEVPQPKAPYGVKGVGEIGLVPTSGAVAA
                     ALHELDGEWRSVLPMRRDTPEPARSGAAR"
BASE COUNT         2119 a         4987 c         5279 g         2334 t
ORIGIN      
        1 tactacccgg gcgcgaccgt cgaggggcga gccctcggag ccgtccgggt gaagctgggc
       61 gacgccccgt cgtcggagct ggcggcgggc gccggcttga tctcggggtc ggtcgacggc
      121 ggcccgacgg tgaccctcgc cggcccgggc caacggatcc tgctcaagat gtcggggacg
      181 acgctccagt accaacgggt cgacaacggt gccggtcccg tcaccaccct gacgtcgggt
      241 ggtggccgct acacgatcac gtgggacgcg ggcagcaacc tcgccgtgtc ggcgacgtcg
      301 cggtcgtaca acgccggcca gctccgcgtc atcccggggt cgggcacctt cgccctggtc
      361 ctcggtggtc tctcgatgca gcagtacctg tacgggctcg gcgagatgcc ggcgtcgtgg
      421 tcgatcgagg cgttgaaagc gcaggcgatc gccggccgga gcttcgccgc ctaccgcctc
      481 gctcaccccc aaggtgccga tttcgacctc tacgcctcgg tcagcgacca ggcctacgtg
      541 ggcatcaccc agacgtcggg ggcgtcgggg gccaattgga tcgcggcggt cgacggcacc
      601 gacggccagg tgctgacgtc ggggggtcag cccatccagg cgttctactc gtcgagcaac
      661 ggtggccaca ccgagcggag cgactacgtg ttcgtggcga cgctgcccta cctggtggcg
      721 gccgccgatc cgtacgacca ggcgacgggc aacgccaact tctcctggac gcggacctac
      781 tccgacgccg aactgggtgc gtggctcaac gccgccggtc ggggcgtcgg gaccgtcacc
      841 ggtgtgagca tcggcggcaa cgtcggcgcg tcgggtcggg tcgacaaggc gacggtgacg
      901 gtgtcgggga ccgatcggac cgtcaccatg accggcaacc agttccgact cgcggtcaac
      961 gtgggggcgc cgtcctcacg cgacctgctg agcaccaagt tcgcgttggg cgacggcccg
     1021 gccgccgccg caccgccggc accggcgccg acggacacga cggcccccga tctcaaggtg
     1081 ctggtcggca gcccgatgcg gttcggcagt tcgggcaaca tctgtgcgtt cgtcatgagc
     1141 gacgaggtcg cgatcacggc gatccacatc cggctcgggc gcaccgatct caagagccgt
     1201 gtcggcggcg tgggcccgac cttccctcag ctcatgtgca tcaacatccc gccgaagctg
     1261 cgcccccggg cggccaccaa ggtcgtgctc acgggggccg ccctcgacgt ggccgtcaac
     1321 atgcggttcg tgcagcagac cgtcacgatc gcccgctgag ccctccccgt tcggccagta
     1381 ggcgggcgac caccgccatg gggagcccga cgatgttgtc ccaggtgccc tcggtgcgct
     1441 caacgaacgc accgccgagg ccctggatcg cgtacgaccc ggccttgtcg agcggctcac
     1501 ccgtcgcgac gtaggcatcg atctcgggcc gacccagggt gcggaaccac acccggctga
     1561 cctcgacgat cccgtacacg tcgccgtcac gcgcaacggc gaccccggtg aggacctcgt
     1621 gggcgcgccc cgacagccgg ccgagcatgg cgccggcgtc gtcggcgtcg gccggtttgc
     1681 ccaggagctc gccgtcgagc gccacgatcg tgtccgcgcc gatcacgacg gcctcgggat
     1741 gtcgttcggc gacgtggcgg gccttgtcgg ttgccaaccg ggcgacgtag gtcgcggccg
     1801 gttcgttcgg cagcggggtc tcgtcgatgt cggccggctc gacggtgaac tcgtagccgg
     1861 cgtcggccat gagctggagg cgacggggtg atcccgaggc caggatcagc accacggcga
     1921 cctacccgcc gctggtgatc gactcggccg cggcgtcgag cgcctcgtcg ctgtcggcga
     1981 gctcgaacca gtgctcgggg agcgacaccg cccgcggccc gttctggtgg ccgcgcttgg
     2041 cccgggggcc ctcgggcgtc atggtgagcg accggtcgag gcgaccgagc gactcggcga
     2101 gctggtcgcg gctctgcagc tggttcagct cacgccggag ctcactcccg accgggtacc
     2161 ccttgaggta ccagcccgtg tgcttgcgga actcccgcac tcccaaccat tcaccggccc
     2221 aggcctggag caggtcgatg tgtcggagca tcgtgtcgca cacctcgccg agcgtcggcg
     2281 gcggcgggat ctcccggccg tcgaacgcgg ccacgaggtc gcggaacagc cacggtcgac
     2341 cgaggcaccc gcgcccgacg accacaccgt cgcacccggt cgcggccatc atcgccacgg
     2401 cgtcggtggc ctcccagatg tcgccgttgc cgagcaccgg gatcgaggtg acgtgctcct
     2461 tgaggcgacc gatcgcctcc caccgggcgg cgccggagta cagctgctcg gcggtgcgtg
     2521 cgtgcagggc gatcatggcg cacccctcgt cctcggcgat ccggccggtg tcgaggtagg
     2581 tgagagggtg gtcgtcgtcg acgcccatgc ggaacttggc cgtgatcggc acgtcgcccg
     2641 ctccggcgac ggcagcggcg atgatgtcgc gcagcaggcg tttgcggacc ggcagcgccg
     2701 aacctccgcc gttcttcgtg accttggggg cggggcaccc gaagttgagg tcgatgtggt
     2761 cgacgtggtc gctgtcgacg agccgtcgaa ccgcctcgcc gacgtggaat gcgtcgatgc
     2821 cgtagagctg gatgctgcgc aggggctcgt cgtcgtcgaa gtgcgcgagc cgttgcgtct
     2881 tcgcgttgcc ctcgacgtag gcgcgggcgg tgatcatctc gctgacgtac aggccggcgc
     2941 cctcggcccg gcacagccga cggaacggag cgttggtcac gccggccatg ggtgcgagca
     3001 ccacgggcac gtcgagggtg atcgggccga tccgcatggc gctcaacgct atcggaggcc
     3061 gctcgcggcc ccggtcgtca gcgaaacgcg tcgcgtggcg tgcgcccggc cgcgagcctc
     3121 gcgtcacatt tcagggggcg gtgccgacga ctcgctcgta gttgaccggg tcggtcgcgc
     3181 cttcggtgca gatgcagacg accgtcgagt cctgtccgag acccatctcg acgaacgact
     3241 ccggcgactc ggcggcgatc gcgagcaggc cgccgaccgt gccggcaccc gtctcgccca
     3301 cgacgatccc gtcgctcgcg agcagacgca tcgcgtcggc cgcgtaggtg tcctcgatac
     3361 cgatcgccca gtcggtgcgg tcgcgcagtg ccggccaggc gatcatcgag acggtgccgc
     3421 agttcatccc cgccatcatc gagtcgtgcg gcccgggcac ggtgaccggc tgaccggctt
     3481 ggatcgatcg gaagaggcaa tcggcggcga gtggttcgac ggccagacga cagggaccga
     3541 cggggggagc ggccggccag tggtcgagcg ccgcctggaa gaacgcgccg acgccgacgg
     3601 ggatcacgat gccgtcgagc gtcacgtcga gtgccgcgag ttgggcgtcg agttccccga
     3661 agatggtcgc gtacccctcg gcgatccacg tcggtacctc ggtgtagccc ggccaggagg
     3721 tgtcgctcac gacgaggcga tcgggcccgg cggtcgctgc cgccgtctcg acggcgaggt
     3781 cgtagtcgcc gtcgacgacc gtgacgcgcg ccccttcgga ctcgatcgcg tcgatgcggg
     3841 cggcgaccgt gccggccggc atgtagatgt cggcacccaa tccgaaccat gccgcggcgc
     3901 gggcgacggc ccgtccgtgg ttgccgtcgg tcgccgtgac gagcgtcaga tcggtgaact
     3961 cggcgaacgc gcggcggaac tcgtcgaacg tggtggcggc cggcgccgtg ccgtgggccg
     4021 ctgcgtatcg ggcagcgagg aggcggtagg tcgcccacga cgcgccgacg atcttgaacg
     4081 ccggcagccc gaatcgctgc gcctcgtgtt tgacgagcac cctcccgagt ccggtccgtc
     4141 gcgccagtga cggaacgtcg acgagcgcgg acggggagta gccgtcgatg agccggtgca
     4201 gggccgtcgg agtgggcagc gcacccggag ggagatcgtg ggggcgagcg gtcgtcgggg
     4261 cggtcggatc gggaaccgca acgactcggg gattccggag catccgcatc ggccgatcgt
     4321 atcggtgacg ggttgatgta ccgatcgtcc gttgctttac gaaacgtaaa gtcgcattta
     4381 gcctgcgcac ccatgagcgc ccccacttcc gccccctcca agctcgggct cgagcgcgag
     4441 atcgtcgacc aggcggtgct cgaacgagcg atctcccggt tccgtgacga gggcatcgtg
     4501 ttgccgacct tcgcccagct cgccgacccg tcgacgatcc ctgccgagat cgtggagcga
     4561 ctcgccggca tcgacccccg ggtgggcgat ccggccaacc tcttccgtgt gcactggcac
     4621 aacaccgcgg ccgccgacgg ttcgttctcg gcgaccgccg atctccccga ccacgtggtg
     4681 ctggggccgg agctgacggg cgtcgaggcc ccgatcatcg tcctgttcgg cgaccggttc
     4741 cccatgatcg acgcgcacaa ggtgctggcg gcctacgcgt gtctggtggc ccgcctcgtg
     4801 acggggcagt tcgacccgac cgagcaccgc gccgtgtggc cctcgaccgg caactacgcc
     4861 cgcggcggag tcgccatcag tcgcatcatg gcgagccgcg gcgtggcggt gttgcccgag
     4921 aacatgagcc gcgagcggtt cgagtggctg gagcgatggg tcgtcgagcc cggcgacatc
     4981 atccgcaccc ccggttcgga gtccaacgtc aaggagatct acgacgcctg cgccgagctc
     5041 gaggcggatc cgaccaactt catcctcaac cagttctgcg agttcgcgaa ctacctcgta
     5101 caccggcacg tgaccggtgc cgcgctcgag cgcgtctacc gctcggtcgc tgcaggtgac
     5161 ggtgggccat cgcagttggc ggcgtgggtc tcggccaccg gttccgccgg caccatcgcc
     5221 gccggcgatc acctcaagga ggtgttcggc acgaagatcg tggcggtcga ggcgctggag
     5281 tgtccgacga tgctccacaa cggattcggc gagcacaaca tccagggcat cggcgacaag
     5341 cacatcccgc tgatccacaa cgtgacgaac accgacgtcg tggtcggcat ctccgaccag
     5401 gcgaccgacg agctggggtt ggcgttcgtg agcgagtcgg gacgtgagct cctgcgccgt
     5461 cgtggcgtcg acgaggccac gatcgcctcg ctcgagcaca tgggcttcag ctcgatctgc
     5521 aacgtgttgg ccgccatcaa gacggccaag gtgctgggcc tcggggccga ccaggccgtc
     5581 atcacggtcg cgaccgacgg gtcgatgttg tacgcctccg agcacgaggc gctcctcgcc
     5641 gagcgattct ccggcgagtt cggcgacgcc caggccgctg cggtcgtcgc ccgtcatctc
     5701 gacggtgccg acgtcgccaa cgtcgaggaa ctcgacacgg ccggccggaa ccgcatcttc
     5761 aacctcgggt acttcacgtg ggtcgagcag cagggcatcg acctcgagac cttcgaggcg
     5821 cggcggcacc agtcgttctg ggacgacctg tcgtcgtacg tgccgcgctg ggacgagctg
     5881 atcggcgagt tcaacgccgc caccggcctc gcctgagtca tcgatgctgc tgacctgccg
     5941 gggctgtggc gccacggtcg acccgctggg cctgtcgccg tgggcgtgtc cgcgccgtga
     6001 cgctgcgggt ctcaccgacg tcgaccacgt gctggttctg tcccgcgaaa gcgtgcagtc
     6061 cgtagcgctt ccgcgggaca gaacgggcgg ggcgggggtc aacccgttcg tgcggtaccg
     6121 ggaacgactc gggtcgtacg ccgcggcgat cgaggccggt tggtccgacg cgcagttcgt
     6181 cgacacggtc gggtcgctcg acgacgcggt cgtggcggtg tgggggagcg ggttcaccgt
     6241 cacacccacc acctcccaac caaccctttc gaccgctgtc ggcgtcgacg ggctgtgggt
     6301 caaggacgag accggcaacg tgtcgggttc acacaaggca cggcacctgt tcgggctggc
     6361 gctgtggctg gcggttcgct cgcccgccga cgacgacgcc cggttggcca tcgccagctg
     6421 cggcaatgct gcgctcggag cgggcgtcat cgctgcggcg atgcgccgtc ccctcgacgt
     6481 ctacgtcccg gtctgggccg acgagtcggt cgtggcgcgg ctcgtcggcc tcggggccaa
     6541 catcctcagg tgcgaacgac gcgagggcga ggtgggcgac ccctgcgtgc tccgcatgcg
     6601 cgaggccgtg gcggggggat cactgccgtt cggatgccag ggacccgaca acggactgac
     6661 catcgacggc ggccgcacga tcgggtggga gctcgcggaa caggcgccgg gtctcgtcgc
     6721 cgtctacgtg cagatgggcg ggggagcgtt ggcgtcgtcg ctggcgcggg gcctggccga
     6781 gggtggatcg gacgctcggg tgatcgcggt gcagaccgaa gggtgcgcgc cgttcgatcg
     6841 ggcctggcgt cgggtcgagc aggtcggttt ggacgaggcg gtgcatcatc ggtcagccgc
     6901 catgtggccc tgggagtcgg agccgaccag cgccgcgacc gggatcctcg acgacgagac
     6961 ctacgactgg gccggcgtcg ctgctggcct cgagctctca cacggtacgt cggtcgtggc
     7021 gccggagtcg gccgtggtgc gggcccacgc ccttgcccac gagcacacga cgatcgacgc
     7081 cgacccgacc ggaaccgccg gcctggccgg cgtgctcacc gcggcgacgg ccggcgccgt
     7141 cggtgtcctc ttcaccgggc tcacccggcg gtgactagag cgtccagccg ctgaaggtgt
     7201 cgatcatcgc cttcacgtcg ccgagcgggt agaggatcgg acaggtcgca ccaccggcga
     7261 tgtagtcggc gacccgcgcc ttggcctcgg cgggagtccc cgacgccgtg agcatctgca
     7321 cgatctcgtc gggcacgagc ttgcttgccg cctcgacctg ctcgtgggtc gccggccagg
     7381 tgaggacctc acccacggag tcgagcaacg actgcgggac gcccgatgcc ttcatgatgt
     7441 ggggctgctg gccgaggtac tgcgtcacca tcatgcgggc catgtcgagg gccgtctgcc
     7501 gatcctcgtg caccgagcac acgaccagct gcgggcggtc gatgtcgtcg atcgagcgac
     7561 cggcacgctc ggcgccgatg gtcaggtgtt ccatggccgt ggcgttgtaa tcggggctga
     7621 cgagatagtt gagcaccaca ccgtcggcga tctcgccggt gagttccatc atcttcatgc
     7681 ccgtggcccc gatgtagatc ggcacgtcct tgggccggcg ctcctggtac acgtagtcga
     7741 gctcgatgcc gtcgaggtgc acgtagtcgc cgtgcatcgt gacggtctcg ttgttgagca
     7801 gcgcccgcac tgcggtcacg acctcgcgca tcacccccaa cggtttggag cgggtgatgc
     7861 cgaccgctgc cgccagcgga tcccaccacg ccccgaggcc gaggatcacg cggccgggcg
     7921 ccaggtcgtc gagcgtcgag aacgtggcgg cgagccgggc agggttgcgg ctccacacgt
     7981 cggcgacgcc ggacccgacc ttgatgcggt cggtcaccga cgcgaacgcc gccatcggta
     8041 ccgtcgactc acgtaccagc cggctctcgg cctgccacac ggcgtcgaat cccttcgact
     8101 cggcgtaccg cgcgaagtcc atgccctcgc ggatcgtgtg ggcgtcctgg aggtagatcg
     8161 cgacgggctg ggtcgttgct tcggctgcca tgtgccgact gtaggttcga cgcgtcagcg
     8221 gccgccgtga cgaaacgcga acgtcatggg acgttcgcga ttcgtcggcg gcggccggcg
     8281 acgattcggg tgtgatcagt actggacgtt gacggcgaag cgcttcccgt cgcactcggc
     8341 gaagacgatc gcgtgcatcg agctggccga tccatcgacg tcgagcacga cgtccgagat
     8401 cgtggcggtt ccggggatca cattgcagcc acggcccggc gaggtcacgt tgacgagaac
     8461 gttgccaggg gcggtgaagt tgtagcgcga caccgtgaag tcaccggcgg tgaatgggcc
     8521 gctgtcgggt ggggcgatct ggagcgtgaa ctgcggcgtg gtgaacgtcg tgacccccgg
     8581 ggtcgcggtg accgtgaact ggctgcccgg atagaaggcc cgcagaccct ggagcgggta
     8641 gttgtcgatg gcgctcgcca cctgcaggta ctgggccggc agtgcggtca gcggcgtcca
     8701 cggcccgcag ccgtcggagg tgaagtaggc gtcgccggcc tcgatgatcg cgagttgcgg
     8761 acccgggccg tagtagatgt cgttgatcac ttcgaggctg ccgtcagcgt tgtccgacgt
     8821 ctcccagtag cagtcggacc cgccgggagc ggtgtagaga cccagctgga tctcggcgcc
     8881 cgtccggtac atgccgtcgc ctgtggcggg catggtgacc ggcgggagat cgcccttgtt
     8941 ccagacgcag ggcccggcgg tggacacact catccctgcg ggaaggttca tgtacagcgg
     9001 gccgccggag ctaccgcgta cccgtcggct cggtcccccg aacacctcga tgatgcaggc
     9061 cgagcccgtc gggaccgtcg tggagtacag gccgggtgac acgagcccgg gggactcggc
     9121 gatctgggcc cggttcgtgg cgccgatcga ggtgagggcc ggggcggcgg gcggcgcggt
     9181 caccggcggg gccggcggcg ccggcggggt cggaggcacc tgcgagcgga gccagttggc
     9241 gacggcgtcg ttcgccgcgg cgatggtcat cagcttgttc caccgcttgt tgctgccgca
     9301 cttgagcacc cacgtgccgt cctgggcgtg ttcgccgacg gttttgcact ttgcgccggc
     9361 ggtccgtcgc ggcaggttct ggcatgcgga gagcaccagg gtcacggcca gcaacgcgcc
     9421 gatcgccgtg gctcgtcgag ttgtgagcgt catcaatcct ccaagttcac gatcgcagca
     9481 ccatgacatc gaggtccccg aaccacaagt gaaaactctg tgtcatcccc ctgaattgtc
     9541 tcggcggacc tgcgacgtag ggtggtttcg atggatgagg accggcccaa gctgcggctc
     9601 gtcgcacccg acgaacgggc gtccgaagcg gacgaagcgc cgcccgagtc gtccacgccg
     9661 ccgccgacac ctccactccc tccttcctcg gggaccgacg acgaccggca ccggttggcc
     9721 tggatgtccg cggcgatccc gctgtgcatc gggatcgcga tcctcgtggt cgggctcgcg
     9781 gttgcgttgc ggctcctgat ggacaccggc gggtccgacg atgccaatgg cgacagcccg
     9841 gcaatcactc gaccggccgg gccgacgagc tcgatcgatc ccctgtcgtt gctgccccgc
     9901 gtcccgacga gtgtgggcga gacgacgacc cttcctgcgc caacggtgcc gacgactgct
     9961 gccccgacga cggtgccgcg caacgtcgac cccggtctca tcgccttgcc ggtgccgggt
    10021 ggtgcgcccg ccgccccggc gtccccccgg ccgccctcgc cgacggctgg gtcggcgagc
    10081 gtgaggatct tcaacgggtt ctcggctggc gaaacgctcg acgtgtggga gatctcgggc
    10141 ccgacgccgg tgcgctacgg ctcgctcccg tacggcggcc tggtcgagat ggtggcggga
    10201 ggccggctgc tgccgagcgg tgacgctctc cagttgcgct tcgtccgacc cggcggcgat
    10261 ccgacgggca cgatccagga cggcacgtcg tcgaagaccg gcccgtgggg tcggaacttc
    10321 acgccggccg acgggtcgtc ggtgaccctc gtgttgtcgc gaaatcccgg gctgcgggtg
    10381 gtcgacatcg acaaccgtcg cgccctggcg gcggttcggt ccgggcaggt ccacgtggtc
    10441 cccgtcgtgt atcacctcgt tctcgagggc acccgtgcca agcaatgggc aggtgacggg
    10501 ctcggttgcc tgggtgtgct gaccacggac acggacgcag aactcgacgt cgacgtgggt
    10561 acggcgttgc ggctggccct cgtcgacgac ccgacctgct cgcgtaccgt caccgacgca
    10621 ctcgtgctcg gggagtcgac cgccgtcgcc gtcgtcggac tcgagattcc gggggatcgg
    10681 gcccagttga tcgcggttcc gttggggtga ggcgcgcgct cacgggtacc ctcggcggcc
    10741 atgcgcagaa aaatcttcct cccgatgctc gtgctggcca ttgcggccgt ggtgcccgct
    10801 tgtgacttcg ccaaggtcgg tgctcgctgc agcgcctcgc agggagctgc gcggaacaac
    10861 acccacgtcc tcttctgtgt gaagggcaag tggaaggcga cggtcacgat cggccaggcc
    10921 gccgagatcg tgatgagctc gtggccggga tcggtgacgg gcgggccggc cgaggttcgc
    10981 gcaacgtcgg gcggggcgtt ccccagcatg acgttcttcg tgaagacgcg gggcggccaa
    11041 ccggctgcaa acgtcgacgt gaagctcacg gcgacgtctg gtcccgggtt ctccggcacg
    11101 agcttcaccg tccgcaccaa cggggccggt gccgtcaaca ccacgccggg tcagtccggc
    11161 atgccgaacg tcaccatggg gtcgggtgtg ggtgacgtga aggtcgacat caacgcgggt
    11221 ccgctgccga caccgatcgg atcgttcgtg atcaaggtca gtggtggcgc tccgtccgtc
    11281 gtcgctgccg tgtcgggtcg tgaccagcag atcaccgctg gcgggacgtt ccagccgtgg
    11341 gtgttcgagg tgcgtgactc gaagggggcc ccgacgatcc cctccaccgt gaaggtgaac
    11401 tcgggtggac tcagtggcgt ctcctacacg caatcgggcg ccacgatcac cgtgacggca
    11461 cctccccagt acgtcgccgc gaaccagcag tcgctcacgg tcgaggtctc gggtgccgag
    11521 ccgttcaaga tgacgacgtt cgcgacgggg tacacgaact ggacgatcgt tgcgggtgcg
    11581 cctgacgacg ccgacatcta cggcgacgac cagtcggcac cggtcaacac ccgattcgcc
    11641 aactccttgg tcgttgacgt gatcgacgcc tacggcaact cggtgaccgg tctccccatc
    11701 acgtacacgt cgatcgacgg cgggtcggga gcgtcgggca cgacgatgcc gaacttctac
    11761 ttcaacccga tcagcgcggt gttcgtcgac gccaacggca cggccggtgc gtggaccgtg
    11821 cgggcggaca tccctggcct gggcacgtac gacttcagcc tgaccaacac cccgtgagtc
    11881 ggctcagagc cggctgaaca gccgcgtggc ctgctcggca gcgtgcgccc ggatctcgtc
    11941 gggatcgaca cgggtcgcac gtccgccgcg cagcgccggc tcgccatcga tgtcgacgtc
    12001 gatcgcgtgg acaccagtcg tgaatgccag tcgccacggg tcgtcggctc cgtcgtagct
    12061 ccaggtgacg gtgtcgtcgc gagcctcggg gaacagggcc catccggtct cgatccaact
    12121 ccaagccgtc gagggggttt cggtgacgtc ggactcccgc aggcggacgt acgcgacgcg
    12181 gaactcgtcc aacatgtcgg cgccgatgcc gtcggtaccg agcgcgaccg gattggcgaa
    12241 gcgagttggt cgcgcgtagc ccacggcgtt gttcatgttc gatcgcgggt tgtgtacgag
    12301 ggtgcctcgc acaccgtggt cgtcgggcag gtggacaccg tggatgatca gccagtcgtc
    12361 gcgcgagagt tcggcgaggc gagcggccgc gtcgacgtcg tcgggaccct cggcgacgtg
    12421 gatgtggagg ccgacgccga ggtcctcggc gaggccggcg gctgcggcga tcgtgtcgtc
    12481 ggtgcaggtg aacgcggcgt ggacgccgac tcggccacgg cccccggccc ggaggtaccg
    12541 ctcgttctcg gcgaggccgc gagccgcgcc gtcggcgccg tggcggtcgg tgacgccgta
    12601 ggcgaggtca caccgaacgc cgacctcggc acacgcctcg gcgatgacgt cgaggctgcc
    12661 ctcgatggcg ttcggcgatt cgtggtggtc gatcagcgcg gtggtgccgc actccagggc
    12721 ttcgagcgcc ccgagccggg ccgacgcccg gatcatctcg aggtcgaggg cggtgtcgag
    12781 tcgccaccag atctgctcga gtaccccgag gaacgatgtg ggtgtctcgg gcggcgccgg
    12841 cattccccgg gccagcgccg agtagaggtg gtggtgggcg cagaccagcc cgggcgtcac
    12901 gttctggagt gctgaggtga cagaaactgt cacctggccg ctccagagcg ggcgggctcg
    12961 ggggtgtcgc gccgcatcgg gagcacggat cgccactcgc cgtcgagctc gtgcagcgct
    13021 gcggcgacgg cgcccgaggt gggaacgagg ccgatctcgc ccacaccctt cacgccgtac
    13081 ggtgccttcg gttggggaac ctcgacgagc tgcacggtga tcgtcggaat gtccttgggg
    13141 cggaggatgc cgagcgaacg cagcgtcatg ttcgtgggga agccgcgttc gtcggacggg
    13201 aagtcctcgc tcaacgcgta gccgaggccc atgtggaccg agccctcgat ctgcccttcg
    13261 cacagcagtg ggttcatggc ccgaccgacg tcgtggatcg ccacgacctc ctcgatcgtg
    13321 cccgtgtcac gatccatgat cacgagctgc gttgcgtagc cgaacgccgc gtggatcgtg
    13381 gggttggggt tctcggggtc gccgagcttc tgggtccagt ccacgatgtg ctcggcgtgg
    13441 tagtcgacgt cgggctgccg gccaccctcg tcggcgaccc gacatgcggc cgagacggct
    13501 ccggcggcca tgagcgtgcc gcggctgccg gtggtctggc cgaagcccag ctcgcgggtg
    13561 gtgtcgacga tcacgtcgat gaggttgggg tcgatgccga gctcctcggc ggcgacctgg
    13621 agcgccaccg tgtgcacgcc ctggcccatc tcggtccagc tgtgccgcac ctcgacgcga
    13681 ccgtccgacg agcggaagcg gatgaccgcc cccgacacct cgcggaaccc gttgccgagg
    13741 ccggagttct tgagcccgag cccgagcccg acgatcttgc cgtcggcgac cgcggcgtcg
    13801 tagcggtcct tgacctcgtc gaggcagacc tcggccccgg cgctcccgtc gtccatgacc
    13861 tgccccggac cccacacctc gccgggccgg atcacgttgc gcttgcggat ctcccagccg
    13921 gagatcccga ccttggcggc gaggcggtcc atgacccctt ccatcgcgaa ctgtgcctgg
    13981 ttggcgccga aaccgcggaa cgctccgcac accgggttgt tcgtgcgtac ggccaccgag
    14041 tcgacgtcga tcgagggaac tcggtacggc cccgatgcgt ggccggccgc ccgctcgagg
    14101 accttcatac cgaccgacgc gtacgggccg gagtcgccga tcatgcggac ccgcagaccg
    14161 gtgagccggc cgtcctcgtc gcagccggcc cagtactcca tgccgatcgg gtgacgtttg
    14221 gcgtgcatga ggaacgattc ctcgcggctg agtgtgcact tcacaggacg gtcgaggagc
    14281 cacgctgcca gggcggtgtg accctggttc gacatgtcct ccttgccgcc gaaggcgccg
    14341 ccgttggaca cgagctcgac ggtgacctgg tcgcgttcga ccccgagcat cgccgcgatc
    14401 tggttgcggt cgtcccacac accctgcccg cccgaataga cgtagagggt gcgctggtcg
    14461 acgcccatgt cgccgggcga ttcggtgccc ggtcggtagg acgggactgc caacgtcgac
    14521 tccggctcga ggaaggcgtg ctcgatgcgc tgggtctgga acgtctcgtg cgcgacgtgg
    14581 gcgctctggg cgagggcgct gtcgacgtcg ccgcgggcgt aggtggagcg ggagagctcg
    14641 ttggtgtcgg tgccccacac ggcgatgtcc gcgccgtcgg cgagcgctgc gaccggatcg
    14701 acgaccggcg tgaggacgt
//
LOCUS       DAOSOA010000038        14668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4129_length_14668_cov_6.649969, whole genome shotgun sequence.
ACCESSION   DAOSOA010000038 DAOSOA010000000
VERSION     DAOSOA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14668
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4129_length_14668_cov_6.649969"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKY13_04255"
     CDS             <1..206
                     /locus_tag="PKY13_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOT79172.1"
                     /translation="MKGGCNFPMGPLALLDLVGLDTSVAILDALYTEFRDPNYATVPL
                     LRRMVSAGQLGRKSGQGFYDYSK"
     gene            222..926
                     /gene="aat"
                     /locus_tag="PKY13_04260"
     CDS             222..926
                     /gene="aat"
                     /locus_tag="PKY13_04260"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOT79173.1"
                     /translation="MDATELTLRWLGLPTCPWEFPPVDSADRSGLVVVGGDLEPETLV
                     TAYCTGIFPMPIGRRRRLGWWSPDPRGIIPVDGLVVSRSLRRSRRRYEIRVDTAFRET
                     MLRCGDPRRPYGWITRGIVQSYERLHLLGVAHSVETWSEDGELVGGLYGVSLGGLFAG
                     ESMFHTAPDASKVALTALVDILGTTTHPLLDVQWTTPHLESLGAVEIAREDYLERLAV
                     ALEQPPPSFTPGPRAG"
     gene            complement(901..1557)
                     /locus_tag="PKY13_04265"
     CDS             complement(901..1557)
                     /locus_tag="PKY13_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79174.1"
                     /translation="MPSTRLTVRLAAALVGALIAVAVFVAMPHEKAARPESVAAIRSD
                     LERRWERWRRTTAAFTETTVRTSGEQELVADAEIAQRFPDRVVRDGDDISAEVGGRQI
                     GCSVTGTSPPQCLDSGPTTDAEARLADELAEFRSLTGGSTPTYRLSRTDTGCFRLRHL
                     VEEFRPRWGDRLDLCFDDRTGVLRTEVTTIGPLTISVARRDIRNDVGDAAFSLPAAPA
                     "
     gene            1642..2199
                     /locus_tag="PKY13_04270"
     CDS             1642..2199
                     /locus_tag="PKY13_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79175.1"
                     /translation="MGFHHVALATPNIEATHRFYTEAMGFELVKAVLAPTPEGGWAKH
                     VFYETGGHGMIAFWDLHVDSLPPVNGAISRGLGLPEWVNHLAFDAPDEAAITSALERW
                     AALGLDVFDINHEFCRSIYTVDPGGTLVEWCMDTRALTAAERERANAVILVDEPDAFD
                     PEPIVAFIPGDPSLRPAWAATPQTA"
     gene            complement(2187..2867)
                     /locus_tag="PKY13_04275"
     CDS             complement(2187..2867)
                     /locus_tag="PKY13_04275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT79176.1"
                     /translation="MSSRWERSDVPRGAEYDARFDRLARAGHDVHGEASFVMGFQPAT
                     VLDAGCGTGRVAIELHRRGVEVVGVDLDRSMLETACEKQPDIEWYRSDLSSFLLPGPD
                     DASVARQFDLVVAAGNVMIFLQPGTEAVTVDRLAEHLRPGGVLVTGFQLTAGRYGVDR
                     LDRDCAAAGLELIERFASWSRDPWMIDSGYAVSVHQRPVVPEPIPAADGEADPAEAAA
                     SGGDDQAV"
     gene            3244..3528
                     /locus_tag="PKY13_04280"
     CDS             3244..3528
                     /locus_tag="PKY13_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79177.1"
                     /translation="MATGGRRARYARRRKLRMDRVEHDLTDDQWRAIQSAWGGCAYCS
                     ATDEPLQRDCVLALSRGGRYTLDNIVPAFLLRSAEIRAMLAAEFPPLSTD"
     gene            complement(3534..4889)
                     /gene="dnaB"
                     /locus_tag="PKY13_04285"
     CDS             complement(3534..4889)
                     /gene="dnaB"
                     /locus_tag="PKY13_04285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT79178.1"
                     /translation="MSNASPGRPVEGRVPPHNVQAEESLLGAMLLKQSAISESVQSVN
                     TGDFYKPAHAHIYDAISTLYAAGEPVDPVTVAAELKRAGLLDAIGGPAVLLSIQARTP
                     AVSNAAHYAKIVEENALLRKLVQTASDIAELGYAPLDDVTKTLDYAESMMYDVAQRRV
                     SDTMVELRDLLDASLDRLEALYERGEAITGTPSGYTDLDVLTAGLQPNALIVVGARPA
                     MGKTSFALGAAANAALRSHKPVLFFSLEMGHLELTQRILGSEGRIDANRLRNGQLNEQ
                     DWSKISRAMGRLGEAPLWIDDNPNLTIMEIRAKARRLKSRVGELGLIVVDYLQLMSGR
                     ANAESRQVEISEISRGLKILARELECPVMALSQLSRALEARADKRPMLADLRESGSIE
                     QDADIVMFVYRDEVYNRDSPDKGTAEIIVSKHRAGPTGMCRLAFLDYCTRFENMAKTQ
                     D"
     gene            complement(5144..5599)
                     /gene="rplI"
                     /locus_tag="PKY13_04290"
     CDS             complement(5144..5599)
                     /gene="rplI"
                     /locus_tag="PKY13_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOT79179.1"
                     /translation="MIDVKLLLRENVAGLGRRGDIVEVSPGYARNFLEPRGLAIKATA
                     GGAAQAEAMRRSQSIKDAKDRESAEEIAKVLVSRTINVKARSAAGGKLFGSVTNVEIA
                     HAIAEQANVELDRKTIHLDEHIKTTGSHQVQIRLHGDVEFPVTVEVTGS"
     gene            complement(5764..6018)
                     /gene="rpsR"
                     /locus_tag="PKY13_04295"
                     /pseudo
     CDS             complement(5764..6018)
                     /gene="rpsR"
                     /locus_tag="PKY13_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(6018..6500)
                     /gene="ssb"
                     /locus_tag="PKY13_04300"
     CDS             complement(6018..6500)
                     /gene="ssb"
                     /locus_tag="PKY13_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT79180.1"
                     /translation="MANGNNVDLIGNVTRDPELRFTPSGQAVATFGLAVNRRWQNRQT
                     QAWEESTSFFDVVCWAQLAENVAESVTKGTRLIVTGRLDQRSWETQDGDKRSKVEVIA
                     DEVGPSLRWATASVSRNERTGGGSYDGGGGGGGGGGGSQSAPTPNAPAPTYDYDEEPF
                     "
     gene            complement(6512..6850)
                     /gene="rpsF"
                     /locus_tag="PKY13_04305"
     CDS             complement(6512..6850)
                     /gene="rpsF"
                     /locus_tag="PKY13_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOT79181.1"
                     /translation="MRAYELMVIVDGDVDDPGVKAVNARVGELVEADGGNIAKTDFWG
                     RRRYAYPINHKLEGIYTVFEIVTPASNLNELDRFLRLADDVVRHKIMRLPEAEAEKRG
                     LLGEPVPAAD"
     gene            complement(7100..7987)
                     /gene="panB"
                     /locus_tag="PKY13_04310"
     CDS             complement(7100..7987)
                     /gene="panB"
                     /locus_tag="PKY13_04310"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOT79182.1"
                     /translation="MSSSRPTELARRPVSVPQVSASKVRAGADPMVMVTAYDAPGARM
                     ADAAGVDMILVGDSLAMVVLGYDDTLQVTVADMAHHVAAVARAKPAALIVGDMPWMSY
                     HVSPADTVHNAAELIRAGAQCVKLEGGAKRIGMVEAIVDAEIPVMGHIGLTPQSMHAM
                     GGFKVQGRESQAALQLVADAKALQHAGCFALVIEGVPDEVAAMITDALDIPTVGIGAG
                     SRCDGQVLVMHDLLGIEDRMAPKFVRRYADVKSVSVEAITRFADDVRSGSFPNADESY
                     HLSAEQSEAMALYGSTSAA"
     gene            complement(8100..10352)
                     /locus_tag="PKY13_04315"
     CDS             complement(8100..10352)
                     /locus_tag="PKY13_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOT79183.1"
                     /translation="MTEDQPRRRPPAKPRPKPTPQARRVPESEARTATKQRAQPKKKA
                     APRKQRASWRVWLRRLTFAAVLLGVASVGIGFVALNSVKIPDPTRTVKTTSFVCLADV
                     ADGECSPSNSVAQFSAGGSNRVIIDIGDMSANLINAVVAAEDRSFFTHGGVDPWGIAR
                     ALYRDLRGSASRQGGSTITQQYVKSVYLTADRTPERKLKEAAIAIKLERKLTKPEILE
                     RYLNEVPLGRGAIGVEAASRAYFDKDAADLTVSESAFIAGLIRAPRYADDPSDPSKPE
                     ENKEATRRRQTVLDAMLEEKYITKEEAEAAAAEPLDQLVLQTPPATTGPIVKKTFADV
                     GGKYITEWTRQQLKAMPNVGETKLLEQGLKVYLTVDPRLQAAAQLSTAQVLNQPDDPS
                     SAMVSIDDNGRIVALIGGQNFDASEVNYALGKAGGGSGRGPGSTFKTIALAEYVNEGY
                     SVKSEKWAPAGWIFPELDDGKPWGIQNYEEKDLGKLTVEQATWESANTVFAQIMLEIT
                     PEKFVEMAQKLGITGNVPAEPSAVLGSGEVSVLDMATAYSTLSNRGTRKPPYIIRRVE
                     AADGTVLYDVSTDPEKQPTPDVIPPAVADTVNSTLAGVINNPLGTGYRARLKTKAVGK
                     TGTTDDYRDAWFAGFTCRITTVVWMGYDNKPGEAARLMDAVRGDKKVTGGSFPADIWK
                     SYMTAATEGARGCAFTPTDAGTNVEPLDDRYAPTTTTTAPPVAAPPPDGAAPPAGGAP
                     AAPTTTAAPG"
     gene            complement(10360..10764)
                     /locus_tag="PKY13_04320"
     CDS             complement(10360..10764)
                     /locus_tag="PKY13_04320"
                     /inference="COORDINATES: protein motif:HMM:NF036456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HOT79184.1"
                     /translation="MLFPQANSSGRPGDTPGTVDYRLDRRRLLRAVRAGEVDREEVCD
                     AQIELMRVGRDYSRAASKPCPICGERTLKNVRFVFGPRLPSQGRCVTSAKELQRLAER
                     AGEHRCYLVEVCIGCRWNHLLSSYLLGPRKSA"
     gene            10869..11705
                     /locus_tag="PKY13_04325"
     CDS             10869..11705
                     /locus_tag="PKY13_04325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT79185.1"
                     /translation="MAEQPPRKSSGSGAPSGPGSRPRHPSAGNLGDRPTLVSPTDAPP
                     AVSTLGYPAGAAFAPGSVRLGPGLTDDSIDRLVGSGDSRRILELGCGDGSNAIALARQ
                     GARVIVVDSSAARLAVARNHAESQDVRIEFHHGDLADLAFVRADQIDLCLAVYSLAEV
                     ADVSRVFRQVHRVLRTEAPFVISLPHPLELMAEADADGEGVRLKRSAFDQSRVAIPGA
                     STQIEAHRITDIFTALTRSNFRVDALLEPPMDPSAEGGFSSPVHEWVPATVIIRGRKQ
                     GI"
     gene            complement(11723..12988)
                     /locus_tag="PKY13_04330"
     CDS             complement(11723..12988)
                     /locus_tag="PKY13_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOT79186.1"
                     /translation="MTDRPADELPAGAEAILAECAPLVDAFAAAGRRLYLVGGLVRDL
                     YGGTALEEVDLDFTTDAPPAEVKAAMGPIAEALWTQGERFGTIAAKVGGRLIEITTHR
                     AEAYDPESRKPAVVFADDVLVDLSRRDFTINALAIELTAGTPRLIDPFDGVGDLRSQV
                     LRTPLQPSESFSDDPLRMMRAARFVARLGVVPAPDVVAAMTAMNARLDVVSHERVRDE
                     LDKLLGVADPRSGLELLVTTGLADRCLPELVDGPLDAVVAARPDAHFRLAVLLAGHGE
                     SAARHRLRSLRHSGQVVDRVARLVSVASSVGGHGPVWTDGELRRLAHRAGAHLADVVE
                     LLGLVGLPNTAVAALRSALADLATRDDLDDLRPALDGQQVMEILGLSPGRDVGAALEF
                     LQQVRFDDGVVDRDTAVRLVSDWWAARPG"
     gene            complement(12985..13995)
                     /locus_tag="PKY13_04335"
     CDS             complement(12985..13995)
                     /locus_tag="PKY13_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOT79187.1"
                     /translation="MMVLVTDERAVNHRAGPHHPESPERLEAVLRATRSGAVRDGIVR
                     LEPRAATPTELARVHDASMLAALDSVRGRSVQLDPDTAVSPESVDIAERGAGAGLTAV
                     EALREGVGSAAFCAVRPPGHHATGERPMGFCLLNNVAVTAAALVAAGERVLIADFDAH
                     HGNGTQDIFYDEPNVLFVSWHQSPLYPGSGALAEVGGPRAEGLTVNLPLVAGTTGDAY
                     LASVQDAVGPIIDRFDPTWLLISAGFDAHRDDPITEMGLSAGDYADLTSLLMEAVPPG
                     RTVAFLEGGYDLGALERSALATMSALLGEPSHPEPPSTGSVGTNAFGRVVDAVVGLHG
                     LR"
     gene            14067..>14668
                     /locus_tag="PKY13_04340"
     CDS             14067..>14668
                     /locus_tag="PKY13_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79188.1"
                     /translation="MTAPLTSSDHLTAALMTRRSLIVGKDRFVVGPWHDDPTLAYVAV
                     APSASVLTSRAAVERCLDQLSHDGYRGAVTAALRSNDAHVFHTAGFELKERLHVLRHD
                     LGGDLGAVDHRLRRARRRDRPDVLEVDRLAFDPFWHLGADGLDEALNATASVRFRVAE
                     EAAVLGYAVCGRALDIGYLQRLAVHPDHSRRGIGAALVVDS"
BASE COUNT         2237 a         5099 c         4994 g         2338 t
ORIGIN      
        1 ccatgaaggg cggctgcaac ttcccgatgg ggccgctcgc gctgctcgac ctcgtcggtc
       61 tcgacacgtc ggtggccatc ctcgacgccc tgtacaccga gttccgcgac ccgaactacg
      121 ccacggtgcc gctgctgcgc cgcatggtgt ccgccggtca gctcggtcgc aagagcggcc
      181 aggggttcta cgactacagc aagtagggaa gcttcgtcgc cgtggatgcg acggagctga
      241 ctctccggtg gctcgggctg ccgacctgtc cgtgggagtt ccctccggtc gacagcgccg
      301 atcgctccgg tctcgttgtc gtcgggggtg acctcgaacc cgagacgctg gtgacggcgt
      361 actgcacggg catcttccca atgccgatcg gtcggcgccg ccgtctcggc tggtggtcac
      421 ccgatccccg agggatcatt cccgtcgacg ggctcgtcgt cagtcggtcg ctgcgtcgtt
      481 cgagacgccg ctacgagatc cgggtcgaca ccgcgttccg ggagacgatg ctccggtgcg
      541 gcgacccccg tcgaccgtac gggtggatca cgcggggcat cgtgcagtcc tacgaacggc
      601 tgcacctgct cggcgtcgcc cacagcgtcg agacgtggtc cgaggacggc gaactggtcg
      661 gtggtctcta cggcgtcagt ctcggtggcc tgttcgccgg cgagtcgatg ttccacaccg
      721 cacccgacgc gtcgaaggtc gcgctcaccg ccctggtcga catcctgggc accaccaccc
      781 acccgctcct cgacgtgcag tggaccacac ctcacctcga gtccctggga gcagtggaga
      841 tcgcccgcga ggactacctc gaacggctcg ccgtcgcgct cgagcagccg ccgccctcgt
      901 tcacgccggg gccgcgggca ggctgaacgc ggcgtcgccg acgtcgttgc ggatgtcgcg
      961 gcgggcgacc gagatcgtga gcgggccgat cgtggtgacc tcggtccgca gcactcccgt
     1021 ccggtcgtcg aagcacaggt cgagacggtc gccccagcgg ggacggaact cctccacgag
     1081 atggcgcagc cggaagcatc cggtgtcggt ccgactcaac cggtaggtcg gggtcgatcc
     1141 tccggtgagc gaccggaact ccgcgagctc gtcggcgagg cgggcctccg cgtcggtcgt
     1201 cggcccgctg tcgagacact gcgggggcga cgtgccggtg acgctgcacc cgatctggcg
     1261 gccgcccacc tccgcgctga tgtcgtcgcc gtcgcggacg acacgatcgg ggaaccgctg
     1321 cgcgatctcg gcgtccgcga ccagctcctg ctcccccgac gtccgcaccg tggtctcggt
     1381 gaacgcagcg gtcgtgcggc gccagcgctc ccagcggcgt tccaggtcgg accggatcgc
     1441 ggcgaccgac tcgggccgag ccgccttctc gtggggcatc gccacgaaca ccgcgacggc
     1501 gatcagcgca ccgaccaacg cggcagccag acgaacggtg aggcgggtcg acggcacggc
     1561 gccacgctat cgaccgtcca ccctcccccg accacgcccg atacgcgaca atcatcggag
     1621 accgagcaag ggggaccagg ggtgggcttt catcacgtgg cgttggcgac gccgaacatc
     1681 gaggcgacgc accgcttcta caccgaggcc atgggattcg agctggtgaa ggccgtgttg
     1741 gccccgactc ctgaaggcgg gtgggccaag cacgtgttct acgagaccgg cgggcacgga
     1801 atgatcgcct tctgggacct ccacgtcgac tcgctcccgc cggtcaacgg cgcgatctcc
     1861 cgaggcctcg gcctgccgga atgggtcaac catctcgcgt tcgacgcccc cgacgaggcg
     1921 gcgatcacca gcgccctgga gcgctgggcg gcgctcggcc tcgacgtgtt cgacatcaac
     1981 cacgagttct gccgctcgat ctacaccgtc gatcccggcg gaacgctcgt cgagtggtgc
     2041 atggacaccc gggccctcac cgccgccgag cgcgagcgcg ccaacgccgt gatcctcgtc
     2101 gacgagcccg atgccttcga ccccgagccg atcgtggcgt tcatccccgg cgatccgtcg
     2161 ctccgcccag cctgggcggc gacacctcag acggcctgat cgtccccgcc ggaggccgct
     2221 gcctcagcgg ggtcggcctc gccgtcggcc gccgggatcg gttcgggaac gaccgggcgc
     2281 tggtgcaccg agacggcgta cccgctgtcg atcatccacg gatcacgcga ccacgacgcg
     2341 aaccgctcga tcagttcgag ccccgccgca gcacagtcgc ggtccaaccg gtcgaccccg
     2401 taccggcctg cggtgagctg gaagccggtg accagcacac cccccggccg aagatgttcg
     2461 gccaggcgat cgacggtgac cgcctcggtg cccggttgca ggaagatcat cacgttgccg
     2521 gctgcgacca ccagatcgaa ctgccgcgcc accgacgcgt cgtcgggacc aggcagcagg
     2581 aacgacgaca gatcggagcg ataccactcg atgtcgggct gcttctcgca tgccgtctcg
     2641 agcatcgagc ggtcgaggtc gaccccgacc acctcgacgc cccgccggtg cagctcgatc
     2701 gccacccgac cggtaccgca cccggcgtcg aggacggtcg cgggttggaa ccccatcacg
     2761 aaactggcct cgccgtgcac gtcgtggccg gcacgcgcca gccgatcgaa ccgcgcgtcg
     2821 tactccgcgc ctcgcggcac atcacttcgt tcccagcgag agctcactcg gccattgtga
     2881 cctccaccac gacccgagcg gggtcagacg aggcagagat cgctcgcgcc gccaccaact
     2941 ggtccgttcg gaccattcct gcgatcgagc attgacgttc tgcgcgtcac atggttcggt
     3001 gctggtgcga gttgatctcg caacagggaa gcactgatcc atcggacgta ctggtcgctt
     3061 gagaccgatg gggagcgaga gcgaaaccga tcccaccaac ggtgtcctga accgggtggt
     3121 gctggctcag gagaccgaac gggccacggc acgttcaacc ttcctggaaa gcgtcgagaa
     3181 cgaggaggcc caacggcctc ctcgttccgc gtcccggcgt cggcgcccac ctacgatcga
     3241 acgatggcca ccggtggacg acgggcgcgg tacgcccggc ggcgcaagct ccgcatggac
     3301 cgggtcgagc acgatctgac cgacgaccag tggagagcga tccaatcggc atggggcggc
     3361 tgtgcctatt gctctgctac cgacgaaccc ctccagcgcg actgtgtgct cgcgctctca
     3421 cggggtgggc gctacaccct cgacaacatc gtgccggcct tcctcctgcg ctctgccgag
     3481 atccgagcga tgctcgccgc ggagttccca ccgctctcga ccgactgatc gactcagtcc
     3541 tgcgtcttcg ccatgttctc gaagcgggtg cagtagtcga ggaacgccag ccggcacatt
     3601 ccggtggggc ccgcacggtg cttggacacg atgatctccg ccgttccctt gtcgggcgag
     3661 tcacggttgt agacctcgtc gcggtacacg aacatcacga tgtcggcgtc ctgctcgatc
     3721 gaacccgact cacgcaggtc ggcgagcatc gggcgcttgt cggcacgggc ttcgagggca
     3781 cgggacagct gtgacagcgc catgaccggg cactccagct cacgagcgag gatcttgaga
     3841 ccacgagaga tctcggagat ctccacctga cggctctcgg cattggcccg gccgctcatc
     3901 agctggaggt agtcgaccac gatcaggccg agctcaccga cacggctctt gaggcgacgg
     3961 gccttggccc ggatctccat gatggtcagg ttcgggttgt catcgatcca caacggggcc
     4021 tcaccgagac ggcccatggc acgggagatc ttcgaccagt cctgctcgtt gagctggccg
     4081 ttgcgcaacc ggttggcgtc gatgcggccc tcggagccca agatgcgctg cgtgagttcg
     4141 aggtggccca tctccaacga gaagaagagc accggcttgt gcgaccggag cgccgcgttg
     4201 gcggctgccc ccaacgcgaa cgaggtcttg cccatggcag gtcgcgcgcc gaccacgatc
     4261 aacgcgttcg gctgcagccc ggccgtgagc acgtcgaggt cggtataccc cgacggggta
     4321 ccggtgatcg cctcaccacg ctcgtagagc gcctcgagcc ggtcgaggct cgcatcgagg
     4381 agatcgcgga gctcgaccat ggtgtcgctc acgcggcgct gggccacgtc gtacatcatc
     4441 gactcggcgt aatcgagggt cttcgtgaca tcgtcgagcg gtgcataccc gagttcggcg
     4501 atgtcgctgg ctgtctgcac cagctttcgg aggagcgcgt tctcctcgac gatcttggcg
     4561 tagtgcgccg cgttgctgac cgctggggtg cgcgcctgga tcgagagcag caccgcgggg
     4621 ccgccgatgg cgtccaggag gccggcccgc ttcagctccg ccgcgaccgt gacggggtcg
     4681 accggttcgc cggcggcgta gagcgtggag atcgcgtcgt agatgtgcgc gtgcgccggc
     4741 ttgtagaagt cgccggtgtt gacgctctgg accgactccg agatcgccga ctgcttgagc
     4801 agcatcgcgc cgaggagcga ctcctcggcc tgaacgttgt ggggcggaac acgaccttcg
     4861 acagggcggc ccggggaagc gttggacatc accgttcatc ctcgtcgcga ggccctgtga
     4921 ggcgtaaggg gaacaacctt ctcatacttg gggacacgat ctcgtcacct ggggaaaggc
     4981 tcgggacagt tggggacact ctgtggatac tcgcgtcgac ccctgacact caccctctac
     5041 agaaccccga gattcaacgt cgaacgcgcc acaggccccg atcggcggac cgatcggggc
     5101 ctgtggagaa gcactggaca actcgtccgg tgcgcgctac gtgttacgaa ccggtgacct
     5161 cgacggtgac cgggaactcc acgtcgccgt ggagacggat ctgcacctgg tgcgaacccg
     5221 tggtcttgat gtgctcgtcg agatggatcg tcttgcgatc cagttcgacg ttggcctgct
     5281 cggcgatcgc gtgcgcgatc tccacgttcg tgaccgaacc gaagagcttg ccaccggccg
     5341 cagagcgggc cttgacgttg atcgtgcggg acacgagcac cttggcgatc tcctcggccg
     5401 actcacggtc cttcgcatcc ttgatgctct gggaccgacg catggcctcg gcctgcgcag
     5461 caccaccggc ggtggccttg atggccaggc ctcggggctc gaggaagttg cgggcgtaac
     5521 ccggagacac ctcgacgatg tcgccgcgac ggccgaggcc ggcgacgttc tcacggagca
     5581 acagcttgac gtcgatcatt ctgcagcctc cgtcacgctc tcctcggcca catcggcgtc
     5641 gccgccggtg gcttcggcgt cggcaccctc gaggggctcg aactcggagt tctcgtcacg
     5701 acgcggacga cgcggacgat cgtcgccgtc acgcgacggc cggccgcgct gggtggtcac
     5761 gcggctcgtg tacggcagga gggccatctc acgggcgttc ttgatcgcct tggcgatctc
     5821 gcgctgctgc tgggtgtcgt tgccgctcac ccgacgggcg cggatcttgg cccggtccga
     5881 gacgaagcga cggaggaggt tgacgtcctt ccagtcgacg tactcgacgc gctcgctgac
     5941 gagcacgctg accttcttct tgccgccacg ccgtgcggcg tcacggggtt tggttgaacg
     6001 tttcggcttc gcggccatta gaacggctcc tcgtcgtagt cataggtggg ggcaggggcg
     6061 ttgggggtcg gagcggactg gctaccgcca ccaccgcctc ctcctccacc gccaccgtcg
     6121 tacgagccac caccggtgcg ctcgttgcgg gagaccgatg cggtcgccca tcgaaggctg
     6181 gggcccacct catcggcgat gacctcgacc ttggaacgct tgtcgccgtc ctgggtctcc
     6241 cacgaacgct ggtcgagtcg accggtcacg atgaggcggg tgcccttcgt gacgctctcg
     6301 gcgacgttct cggcgagctg ggcccagcag accacgtcga agaaggaggt ggactcctcc
     6361 cacgcctggg tctgacggtt ctgccagcgc cggttgacgg ccaacccgaa ggtcgccact
     6421 gcctgacccg acggcgtgaa gcgcagttcg ggatcgcggg tgacgttgcc gatcaggtcg
     6481 acgttgtttc cgtttgccat gagattcctc ctcagtcggc agccgggacc ggctcaccga
     6541 gaagcccacg cttctcggct tcggcctcgg gcagacgcat gatcttgtgg cggacgacgt
     6601 cgtccgcgag acggaggaag cgatcgagct cgttgaggtt cgacgccggg gtgacgatct
     6661 cgaacaccgt gtagatgccc tcgagcttgt ggttgatcgg gtaggcgtag cgacgccggc
     6721 cccagaaatc ggtcttcgcg atattgccgc cgtcggcttc gacgagctca ccaaccctcg
     6781 cgttgactgc tttgaccccg ggatcatcga cgtcgccgtc gacgatcacc atgagttcat
     6841 acgcgcgcat gtacatgtac ctccctgtgg acctcggcca cgcagggccg gagggccccg
     6901 gacggtccgg ggcagggcgt cgatcgacag acgtcgaaac gaccgggcaa ccctacccgc
     6961 tcacgaggac gggcaccatc tggaccggtg ccgaaggaag ttcgacgtca gcggaccgct
     7021 ccggtcgcct cacgtcgaac ttcggccgga cgcgacgatg ggcgccctgt cgggcgccca
     7081 tcgtgtgcac gagtcgtgat caggctgcgg acgtggagcc gtagagggcc atcgcctcgg
     7141 actgctcggc gctcaggtgg tagctctcgt ccgcgttggg gaacgaaccg ctgcgcacgt
     7201 cgtcggcgaa gcgggtgatg gcctcgaccg acaccgactt cacatccgcg taccggcgca
     7261 cgaacttggg ggccatgcgg tcctcgatgc cgagcaggtc gtgcatgacg agcacctggc
     7321 cgtcacaacg cgagccggca ccgatgccga cggtggggat gtcgagcgca tcggtgatca
     7381 tcgcggcgac ctcgtcgggc accccttcga tcacaagggc gaagcagccg gcgtgctgga
     7441 gggccttggc gtcggcgacc agctgcagcg cagcctgtga ctcgcgaccc tgcaccttga
     7501 agccgcccat ggcgtgcatc gactgcgggg tgaggccgat gtggcccatg acggggatct
     7561 cggcgtcgac gatggcctcg accatgccga tgcgcttggc gccaccttcc agtttcacgc
     7621 actgggcacc ggctcggatg agctcggcgg cgttgtggac cgtgtcggcc ggcgacacgt
     7681 ggtagctcat ccacggcatg tcacccacga tgagggctgc gggcttggcc cgagcgaccg
     7741 cggcgacgtg gtgtgccatg tcggccaccg tcacctggag cgtgtcgtcg tagcccagga
     7801 ccaccatggc gagggagtca ccgacgagga tcatgtcgac cccggccgca tcggccatac
     7861 gggcgccggg ggcgtcgtag gccgtgacca tcaccatcgg gtcggcaccg gcgcgcacct
     7921 tcgaggccga aacctgagga acggacaccg gtcgacgggc gagctcggta ggacgagaac
     7981 tgctcatgcg agcacctcca ttgtgaggtc cggcgccatt cagctaccgg ccgatgtccg
     8041 aactgtatga cgggagcccc ggaaacacca gtggcaatcc tcgcccgccc gggggattct
     8101 cagccggggg cagccgtggt ggtcggcgcc gcaggtgcgc cgccggcggg aggtgcggcg
     8161 ccgtcgggtg gcggggctgc aaccggcgga gcggtggtgg tcgtcgtggg ggcgtacctg
     8221 tcgtcgaggg gctcgacatt cgtcccggca tcggtcgggg tgaaggcaca gccccgagct
     8281 ccctcggtcg ccgccgtcat gtagctcttc cagatgtcgg ccgggaacga tccgccggtg
     8341 accttcttgt cgccgcgaac cgcgtccatg agacgggccg cttcgccggg cttgttgtcg
     8401 taccccatcc acaccacggt cgtgatccgg caggtgaacc cggcgaacca ggcgtcgcgg
     8461 tagtcgtcgg tcgtgcccgt cttgccgacg gccttggtct tgaggcgggc ccgatagccc
     8521 gtgcccaacg gattgttgat gacgccggca agcgtcgagt tcaccgtgtc ggccaccgcc
     8581 ggtgggatca cgtccggtgt cggctgcttc tcgggatcgg tgctgacgtc gtagaggacg
     8641 gtgccgtccg cggcctcgac acgacggatg atgtagggcg gcttgcgggt acctcggttc
     8701 gacagcgtgg agtaggcggt ggccatgtcg agcaccgaca cctcgcccga gccgagcacg
     8761 gccgacggct cggcgggcac gttgccggtg atgcccagct tttgggccat ctcgacgaac
     8821 ttctcggggg tgatctcgag catgatctgg gcgaacacgg tgttggccga ctcccacgtg
     8881 gcctgttcga ccgtgagctt cccgaggtcc ttctcctcgt agttctggat tccccagggc
     8941 ttgccgtcgt cgagctcggg aaagatccag cccgctggcg cccacttctc cgacttcacc
     9001 gagtagccct cgttcacgta ctcggcgagg gcgatcgtct tgaacgtcga cccgggccca
     9061 cgcccactac cgccgcccgc cttgccgagc gcgtaattga cctcggacgc gtcgaagttc
     9121 tgcccaccga tcaacgccac gatgcggccg ttgtcgtcga tcgacaccat ggcgctcgac
     9181 ggatcgtcgg gttggttgag cacctgcgcc gtcgacagct gcgcggccgc ttggaggcgc
     9241 gggtcgaccg tcaggtacac cttcaggccc tgctcgagaa gtttcgtctc gccgacgttg
     9301 ggcatcgcct tgagctgctg gcgcgtccac tcggtgatgt acttgccgcc cacgtcggcg
     9361 aacgtcttct tcacgatggg acccgtcgtg gcgggcggtg tctgcaacac cagctgatcc
     9421 aagggctcgg ccgccgcggc ctccgcctcc tccttggtga tgtacttctc ctcgagcatg
     9481 gcgtcgagca cggtctgacg gcggcgggtc gcttccttgt tctcctccgg cttgctcgga
     9541 tcggagggat cgtcggcgta ccgcggcgca cggatcagcc cagcgatgaa ggccgactcc
     9601 gagacggtca ggtcggcggc gtccttgtcg aagtaggccc gcgacgccgc ctcgacaccg
     9661 atcgcacctc ggcccaacgg gacctcgttg aggtaccgct cgaggatctc gggcttggtc
     9721 agcttccgct ccagcttgat ggcgatcgct gcttccttga gcttgcgctc cggcgtgcgg
     9781 tcggcggtca ggtacaccga cttcacgtac tgctgggtga tcgtggagcc gccctgccgc
     9841 gacgccgagc cgcgcagatc gcggtagagc gcacgggcaa tgccccacgg gtcgacgcct
     9901 ccgtgggtga agaacgagcg atcctcggcg gcgaccacgg cgttgatcag attcgccgac
     9961 atgtcgccga tgtcgatgat cacccggttg ctgccgccgg cggagaactg cgcgaccgag
    10021 ttcgacggcg agcactcgcc gtcggcgacg tccgcgaggc acacaaagga agtggtcttc
    10081 acggtgcgcg tcgggtcggg gatcttgacc gagttgagcg cgacgaaccc gatgcccacc
    10141 gaggccacgc cgagcagcac cgccgcgaac gtcaaccgac ggagccacac gcgccacgac
    10201 gcccgctgct tgcgcggcgc cgccttcttc ttcggctgcg cacgctgctt ggtcgccgtg
    10261 cgagcctccg attcgggaac gcgccgagcc tgaggggtcg gcttcggccg aggtttcgcc
    10321 ggaggacgcc ggcggggctg atcttcggtc atcgccgcgt cacgccgact tccgcggccc
    10381 caacaggtag ctcgacagga ggtggttcca gcggcagcca atgcacacct cgacgaggta
    10441 gcagcgatgc tccccggccc gttcggcgag tcgttgcagc tccttggccg aggtcacaca
    10501 ccgaccctgg gacgggagtc gcgggccgaa cacgaaccgg acgttcttga gggtgcgctc
    10561 gccgcagatg gggcacggtt tcgacgccgc ccgcgagtag tcgcgaccga cgcgcatgag
    10621 ttcgatctgc gcatcgcaga cctcttcgcg gtccacttca ccggcccgca ccgcacgaag
    10681 aagccgccgg cgatcgagcc ggtagtcgac cgtgcccggg gtgtcacccg gtcggcccga
    10741 cgagttcgct tgcggaaaca gcacctcggc aggctatcga cccccggtga cggagtacac
    10801 ggcggggacg ctcacccacg gcgcgagggg tccccacgcg ccgagcggcg atcagcaaga
    10861 gttgtcccgt ggcagagcaa cctcccagga agtcctcggg tagcggcgcc ccctcggggc
    10921 ccggctcccg gccgcgtcac ccgtcagcgg gcaacctcgg cgaccggccg acgctcgtct
    10981 cgccgacgga cgccccgccg gcggtgtcga ccctcggcta tccggccggg gcggcgttcg
    11041 cccccggctc ggttcgcctc ggtcccggcc tgaccgacga ctcgatcgac cggttggtgg
    11101 gcagcggcga cagccggcgc atcctcgagc tcggctgcgg cgacggcagc aacgcgatcg
    11161 cgctcgcccg gcagggtgcc cgtgtcatcg tggtcgactc gtcggcggcc cgcctggcgg
    11221 tcgcccggaa ccacgccgag tcccaggacg ttcgcatcga gttccatcac ggcgacctcg
    11281 ccgacctcgc gttcgtccgc gccgaccaga tcgacctctg tctcgcggtg tactccctcg
    11341 cggaggtggc cgatgtctcg cgcgtgttcc gtcaggtcca ccgcgtactg cgcaccgaag
    11401 cgccgttcgt catctccttg ccgcacccgt tggagctcat ggccgaggcg gacgccgacg
    11461 gcgagggggt tcggttgaag cggtcggcgt tcgaccagag ccgtgttgcg attcccggcg
    11521 cctcgaccca gatcgaggcg caccgcatca ccgacatctt cacggcgctc acccggtcga
    11581 acttccgcgt cgacgcattg ctcgagccgc cgatggaccc gtcggcggag ggtgggttct
    11641 ccagcccggt gcacgagtgg gtaccggcca cggtgatcat ccggggccgc aaacaaggca
    11701 tctgagcagc ccgggcggcc ggctaccccg gtcgggctgc ccaccagtcg gagacgagcc
    11761 ggacggcggt gtcacggtcg accacgccgt cgtcgaaccg gacctgctgg aggaactcga
    11821 gggccgctcc cacgtcgcgt ccgggtgaca ggccgaggat ctccatgacc tgctggccgt
    11881 cgagcgccgg ccgcaagtcg tcgaggtcat cgcgcgtggc caggtcggcc agcgcggacc
    11941 gcagcgccgc cacggcggtg ttcggcagcc cgacgagacc gagcagttcg accacgtcgg
    12001 ccaggtgcgc cccggcccgg tgggcgagcc ggcgcagctc accgtcggtc cagaccggac
    12061 cgtgaccgcc gaccgacgat gcgaccgaga ccagccgggc cactcgatcc acgacctgcc
    12121 ccgagtgtcg caaggaccgg agtcgatgtc gcgccgccga ttcaccgtgt ccggcgagca
    12181 ggacggcgag gcggaaatgc gcatcgggcc gggccgcgac cactgcgtcg agggggccgt
    12241 cgaccagctc cggaaggcag cggtcggcca gaccggtggt gaccagcagc tcgagaccgc
    12301 tgcggggatc ggccacaccg aggagcttgt cgagctcgtc gcgcacccgc tcgtggctca
    12361 ccacgtcgag gcgagcgttc atggccgtca tcgccgcgac gacgtcgggt gccggcacga
    12421 ctcccaggcg ggcgacgaag cgggcggcgc gcatcatgcg gagcgggtcg tcggagaacg
    12481 actccgacgg ctgcagcggg gtacgcagca cctgggagcg gaggtcaccg accccgtcga
    12541 acgggtcgat gagacggggc gtgcccgccg tcagctcgat cgccagggcg ttgatcgtga
    12601 agtcccgtcg cgacagatcg accaggacgt cgtcggcgaa cacgaccgct ggcttgcggc
    12661 tctcggggtc gtaggcctcg gcccgatggg tcgtgatctc gatgagccgc cccccgacct
    12721 tcgccgcgat cgtgccgaac cgctcgccct gggtccagag cgcctcggcg atcggcccca
    12781 ttgctgcctt cacctccgct ggtggggcat cggtggtgaa gtcgaggtcg acctcctcca
    12841 gggctgtccc gccgtacagg tcgcgcacca gcccgcccac gaggtagagg cgacgccccg
    12901 ccgcggcaaa ggcgtcgacc agcggagcgc actcagcgag gatcgcctcg gcaccggcgg
    12961 gcagttcgtc cgcgggccga tcggtcaccg cagaccgtgc agtccgacga cggcgtccac
    13021 cactcgtccg aaggcgttgg tgccgaccga ccccgtactc gggggctctg gatgggacgg
    13081 ctcgccgagc agcgccgaca tggtggcgag cgccgaccgt tcgagggcac cgaggtcgta
    13141 gccaccttcc aggaaggcca ccgtgcggcc ggggggaacc gcttccatca gcaggctcgt
    13201 caggtcggcg tagtcaccgg cgctgagacc catctcggtg atcggatcgt cgcgatgggc
    13261 gtcgaacccc gccgagatca acagccaggt cgggtcgaac cggtcgatga tcggccccac
    13321 cgcatcctgc accgaagcga ggtaggcgtc gccggtggtg ccggccacca gcgggaggtt
    13381 cacggtgagg ccctcggcac gcggcccccc gacctcggcg agtgcaccgc taccgggata
    13441 gagcggtgac tggtgccacg acacgaacaa cacgttcggc tcgtcgtaga agatgtcctg
    13501 ggtcccgttg ccgtggtggg cgtcgaagtc ggcgatcaac acacgctccc cggctgcgac
    13561 cagggccgct gccgtgacgg ccacgttgtt gaggagacag aagcccatgg ggcgctcccc
    13621 ggtcgcgtgg tgccccggcg gacgaacagc gcagaacgcc gccgacccga caccctcccg
    13681 cagggcctcg acggcggtga ggcccgcacc cgcaccacgc tcggcgatgt cgacggattc
    13741 cgggctcacg gcggtatcgg ggtcgagttg caccgatcgg ccccggacgg agtccaacgc
    13801 ggcgagcatc gacgcatcgt ggacgcgggc cagctcggtc ggggtcgcgg cgcggggctc
    13861 gagccgaacg atcccgtcgc gcaccgcccc cgaccgggtc gctcggagga ccgcctcgag
    13921 ccgctcaggc gactcggggt ggtgggggcc ggcacggtgg ttgaccgccc gctcgtccgt
    13981 gaccaagacc atcatcgtcg gcaagcgtac gatgagcgcc gacgtggcgg tgacccacgt
    14041 ctcgatccag cccgaacgcg ccaccgttga ccgcgccctt gacctcgtcc gaccacctca
    14101 cggcagcgct tatgacccgt cgatcactca tcgttggcaa ggaccggttc gtcgtcggtc
    14161 cctggcacga cgatccgacg ttggcgtacg tcgccgtggc gccgtcggcc tcggtgctca
    14221 ccagtcgggc ggcggtcgag cggtgtctcg accagctgtc acacgacggc taccgcggcg
    14281 ccgtgaccgc cgccctccgg tcgaacgatg cccacgtgtt ccacaccgcc gggttcgaac
    14341 tgaaggagcg gctgcacgtc ctccgtcacg acctcggcgg cgacctcggg gccgtcgatc
    14401 accggctccg ccgtgcccgt cgtcgtgatc gacccgacgt cctcgaggtc gaccgtctgg
    14461 cgttcgatcc gttctggcac ctcggcgcag acgggctcga cgaggcgctc aacgccacgg
    14521 cgtcggtgcg gttccgggtc gccgaggagg cggccgtcct cggatacgcg gtctgcgggc
    14581 gcgcgctcga catcggctac cttcagcgcc ttgccgtgca ccccgaccac tcccgacgtg
    14641 gcatcggcgc agcgctcgtg gtcgactc
//
LOCUS       DAOSOA010000039        14639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4146_length_14639_cov_5.681706, whole genome shotgun sequence.
ACCESSION   DAOSOA010000039 DAOSOA010000000
VERSION     DAOSOA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14639
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4146_length_14639_cov_5.681706"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKY13_04345"
     CDS             <1..418
                     /locus_tag="PKY13_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOT79189.1"
                     /translation="SVIEQGKFEEDFGGGVSQWATTTFNAAFFAGLDIPEHKAHSIYI
                     SRYPFGREATLAYPSVDLKIKNNTPYGVVIYPTYTNTSITVQLWSTRFAAGAQTAISK
                     TSGCGDVTLTRTRVFVDGHTDTDKFKANYNCNPPEH"
     gene            607..2010
                     /locus_tag="PKY13_04350"
     CDS             607..2010
                     /locus_tag="PKY13_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOT79190.1"
                     /translation="MNDDMLYEDRMSDADALMWNIEKDPALRSTITAVFTFDRAIDRD
                     ILTHRFERLSRVIPRLRQRVRSNPLSAAPPRWETDPYFDLGFHLWWVKAPGRATMRDL
                     LSIAEPVAMSGFDRARPLWRAVVVEGLPGKKSALIMKVHHAITDGVGGMRLQLELLDM
                     EPDAPEREMPPEPTIHVMSQPERFADALDHQTRHQLGLARRLANGVLGSAASALTDPI
                     GTLAAGSELASSVGRVLRPASHPMSPLMVGRSLSSRFDTITVPLDGAKAAAKVAGGKL
                     NDAFVGGVARGLYKYHLAHGIDCDELRMAIPINVRTAELENVAGNAFVPARLELPIDA
                     EDPVDSMRQVHSMVDGARMEPANDLVEPVASILNRLPTTAVTALFGSMVKAVDFTTSN
                     VPGAPFPVYLGGAKMEAQFPFGPLAGAALNITLLSYQNDLNIGIASDPAAVPDPDVLV
                     RSLQAGFDEILAVASKK"
     gene            2021..3229
                     /locus_tag="PKY13_04355"
     CDS             2021..3229
                     /locus_tag="PKY13_04355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT79191.1"
                     /translation="MSDVDVLIVGAGPAGTAAAITLTRSGRSVLVVDKASFPRDKICG
                     DGLTTSALRHLEGLGVEPHQIDSWFPVHDVVVRAPYGVERRFSLPRGQGLYAVVATRR
                     DLDAALVDRARKEGAEIAEGAALTSIANTADGVVAEVEGLGEVRARFAVGADGMWSPT
                     RKLLGAGVEGYRGEWHAFRQYVGGVSGRAAIDLVVFFEPDFLPGYFWSFPLPGGRANI
                     GFGIQRGGTHSIQDMKGLWQDLLQRPHVRELLGPDVEPLEPHRAWPIPCRPGELSTSA
                     GSVLFVGDAVGACDVMTGEGIGQALQTGEMAARHINASWHDPALVASGYEAEFLTELG
                     NDAAMSSLLVRALQHRKGAAAALAVAGTSDWTRRNFIRWLFEDSPRGIMYRPSLWRRG
                     ALSGPGAYRT"
     gene            complement(3267..4007)
                     /locus_tag="PKY13_04360"
     CDS             complement(3267..4007)
                     /locus_tag="PKY13_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79192.1"
                     /translation="MSSRTMLLIATGVAGVAAWAGIAFTGSAGSQVSSPPSGFDLERP
                     YDAEIVETHRSAGEVTKQLRHEVHFVDSENYDVTSTKEVGDDFDVTDEVRRPGSYRRI
                     SPDAYYDVADVVPDADEQARLSDELGPDAAGAAVQELARSGQMQRHDGVWADEKDVEA
                     GARPVPQAAGTVLGIKRDPASNNPTDLKLVYQRNGDVTVGTVSDGPWSLTITIDAQGR
                     PLKATEVVERDREITYEFERFSYRTPAT"
     gene            complement(4075..4758)
                     /locus_tag="PKY13_04365"
     CDS             complement(4075..4758)
                     /locus_tag="PKY13_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79193.1"
                     /translation="MASIEGLKRKLNEYKPTVVSAFVVYVTPRASPIDPDDIDPKFTI
                     VEVNTPDDPILPKITSSKRQLKIAQQAMRDGRWNVIVALNEDMEPVGRIWETVRTETA
                     LANGVPRMKLAADEFLMFDLWVDREYRRGGLANTMADFFFRRDPPETSPRAGGNGYGF
                     VAYDNVPSILWHHSVGFQIAQTMNYLQIGPFIKWKIPFSEMPRFGPMSRKGRHTDPDK
                     KIFGPPLFP"
     gene            complement(4823..6334)
                     /locus_tag="PKY13_04370"
     CDS             complement(4823..6334)
                     /locus_tag="PKY13_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOT79194.1"
                     /translation="MADVAESGADGTVDGTSGDDDLSLGRKAAGGFVWALVGFALMQV
                     GSFATYTIATKVLGADGIGVVGIALTLVFWIDILLGVGMGASLIFEQEKGQTERVAVA
                     FTVNMMVAVVLSVAIFFGAGAIDDFLGAGNPNLFRVVAALVLVKGLGQVPDAMLKRDL
                     DFKRRAKADFTRSLGRFVISVSLLKAGVGPIAMVIGVLSAEIAATVVTWALVRFKPTL
                     RFDLKIAGQMLRFGSAMFAAQLVGMLWLQGDYLVLSRHFNSKSRQFGEYYTAFRLPEL
                     VLGSVYNLFSNVAFPAYAAAREAGPEALRNASLKSLRLLCMFGFAAGVGMSLIARDFI
                     TVVYPEVLGAIPIMQLLCVAGGFVGVGYASGDLFNAMGKPRLGLYFSLIFVPILMGGF
                     LLVVDRGVIAIAAVHVAVIIPYSAFRIQVANRLLGTTWKDSLIALRPAAALVIGIGAF
                     AWPVRLTLDAGWGSLFAVFFAGLVGGFAGLAIGDRATLVELGEGGQKVLARFR"
     gene            6255..7409
                     /locus_tag="PKY13_04375"
     CDS             6255..7409
                     /locus_tag="PKY13_04375"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT79195.1"
                     /translation="MRPRLRSSSPDVPSTVPSAPLSATSATSAPDRVLRQAGRDRPWY
                     RPAHPQREQASPATVRRMRTRLTEILQIEHPVMLAGMGGVSYSELAAAVSNGGGFGCL
                     GASTMSNDRMEQEIAATKALTDRPFGVDLLTAAPGDMEAQVELIVAGGATVFAAGLGV
                     PSKVIDQCHDNNIIVVNICGKVRHAIAAVEAGCDIVVAQGTEAGGHTGQVATMALVPQ
                     VVDAVGDRVPVVAAGGLYDGRGLAAALALGADGVWVGTRFIATPEAYGVRGYKDLLLG
                     AKEDSTVISRGFTGKTCRVLRNDYTQYWEEHPDELQPFPQQFIRSMNDGANHLGLGLD
                     DDRVDPNKEFFPAGQGVGGINELVPAAELVQRFVTEAEEILDRVASFARN"
     gene            complement(7496..8527)
                     /locus_tag="PKY13_04380"
     CDS             complement(7496..8527)
                     /locus_tag="PKY13_04380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT79196.1"
                     /translation="MRISMQMPYAGGFEQSVQQTVALEKAGLDIVYVAEAYSFDAVSA
                     MGFLAARTETVQIAAGILPIYTRTPTLLAMTAAGLDHVSDGRCIMGLGASGPQVIEGF
                     HGVKYDAPLGRTREIIEICRKVWAREEKLTYDGKYYQLPLPPEQGTGLGKPLKIINHP
                     KRSDIPIHIASLGPKNLELTAELANGWLPIFYYPEKAKDAFGPSLDKGYAKRSPELGP
                     MDVVAGGLLAVGDDVKGVLDMARPLMALYVGGMGAVGQNFYNALITRYGYEEEAKQIQ
                     ELYLAGKKEEAAAAIPTELLEHTNLVGPEGYVKERIAAYKEAGVTVLNVTPFDADQPA
                     LIEKVKGWL"
     gene            complement(8530..8790)
                     /locus_tag="PKY13_04385"
     CDS             complement(8530..8790)
                     /locus_tag="PKY13_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79197.1"
                     /translation="MAKRTGNELLLHDRVISKVDLPRVPAGTPGKVILTSGWDHKDGR
                     DWQRFRVLFEIGGPNGTDVGSLNRGTLQRIDKHGKVIEGENN"
     gene            complement(8771..9610)
                     /locus_tag="PKY13_04390"
     CDS             complement(8771..9610)
                     /locus_tag="PKY13_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOT79198.1"
                     /translation="MRALITGGAGLLGSELARCTPAGWSTEVTIRERPASAGLKAHRI
                     DLAQPLAAFDLVERRRPDVVIHTAYSKTDQRLTVDSTTEVAAACAALDIALVHVSTDA
                     LFDGDHAPYAESAEPAPIHAYGRAKALAEAAVREACPDAAIVRTSLILAPDGTDSTSA
                     WVVERLRSGERVTLFDDEFRAPILVDDLASQLWDIVSLPAGERSGVWHLVGPERLSRV
                     DVGRILCRRFGLDESLIDVASAASMGEPRPRDVTLSSERSSRLPSQARPIGKVSAHGE
                     TNR"
     gene            complement(9618..10772)
                     /locus_tag="PKY13_04395"
     CDS             complement(9618..10772)
                     /locus_tag="PKY13_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT79199.1"
                     /translation="MDLEMPSEDDPRRIEVRAWIADHPSPTGRELAEAGLVAPHWPRP
                     WGLDADPIHQLIIDDELKRARIRRPDNMIGIGWAGPTLVHAGTPEQKDRYLFPLLAGE
                     EIWCQLFSEPDAGSDLASLGTRAVRDGDEWVVNGSKIWNSGAQNSQFGILIARTDPDA
                     PKHQGISYFVCPMDTPGVEVRPVREMTGVALFNEVFFTDVRLPADAIVGDVNRGWGLA
                     KVTLGNERVSLSGGGALWGMGPDAYALFDIVRERGGVTDPVRRQELASLYIEAEILRL
                     IRLRTVSAAIKGEAPGPEASIRKLLADEHGQKIMGLAKDLVGTGAMLTGEGPMGAPVD
                     MWHFGYLFARALTIGGGTTQVQRNILAERVLGLPHDIDPHEGRPWTESRR"
     gene            complement(10777..11940)
                     /locus_tag="PKY13_04400"
     CDS             complement(10777..11940)
                     /locus_tag="PKY13_04400"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT79200.1"
                     /translation="MNPDPLARRLRGFGTTIFAEMTALANETGAINLGQGFPDTDPPP
                     EVADAAVAAIRDGHNQYPPGPGILPLREAIADHQERFYDLRVDPGTDVLVTTGATEAI
                     AAAMISLLDPGDEVVMFEPYFDSYAAAVAMAGGVRRVVPLVLVDGAWGFDPDVLRAAI
                     TPRTKLLLINTPHNPTGKVLTQAESEQIAAVAIEQNLLVVTDEVYEHLVYDGVHIPLA
                     TLPGMAERTLTISSGGKTFQCTGWKVGWIVGPEPLVTAVRTSKQFLTYVSGGPLQYGI
                     AVGLRLGDDHFATLRERVRSERDRLAVGLAAAGFDVPTAEGGYFLTTDVADLGHTDAR
                     TFCLELPATAGLVAIPSSVFYDDADRGRTFVRWAFCKRSEVVASAVDRLQRLR"
     gene            12025..12741
                     /locus_tag="PKY13_04405"
     CDS             12025..12741
                     /locus_tag="PKY13_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79201.1"
                     /translation="MPRSRKVLVNRRTLASIALAAACVVGLTACDIHSPDMELGFTAN
                     PDGTTTVTGLAADQDQAGTKLLVVVTVDGTTNNAVAANLPAAAWSPQRPFKCGNKDKD
                     GNEQTCFLTVRLGEDHRFTATFTNPTDPEKLVCITPLGVAVKTPTGEGLVTQHTKQCF
                     TAAKIAELTGTTPTTAPPTPVPPTTAAPTTSAAPTTTTEATTTTTMPAPTSTTSTTIF
                     DPGPGPGGPCGPDPMDLSCI"
     gene            12857..14506
                     /locus_tag="PKY13_04410"
     CDS             12857..14506
                     /locus_tag="PKY13_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOT79202.1"
                     /translation="MSGGWNFAEIWERIADKFPDAQAQVQGDRRFTWTEFDRRADGIA
                     RTLLDHGAGHQDKVALYLYNGPEYMETAFACYKASLVPVNTNYRYIDDELTYLWDNAD
                     AVAVVFHGEFTPTIERIRSRVPDVKVWLWVDDGNGDCPEWAIPYEDAAATETERVVGA
                     KGRSGDDILMIYTGGTTGMPKGVMWRQDDLIHATCTTGLPILNGEQSEVGYDALLEAI
                     AAPGAPGLPACPLMHGTGWFTANIFLVQAGSMVSLVDRRFNVDEILDTVEREGVGAMT
                     IVGDSFAKPIVAALDANPDRWDLSSLVLITSSGVMWSEPVKHALLAHHPGMLLIDVFS
                     SSEAIGLGQSISAAGAESHTAKFSLGPSSRVISDEGRDVVPGSGEKGRVAVRGFTPIG
                     YYKDPEKSAATFITIDGARYSMPGDWATVEEDGTISLLGRGSVCINTGGEKVFPEEVE
                     EALKLHPTVNDAVVVGVPDDKFGQAITAVVEPAAGASIDEATLIAEVKAHLAPYKAPK
                     RVLVVDTIGRAPNGKVDYKRHLTYALDQLGIEPSPIPVANA"
BASE COUNT         2405 a         5168 c         4843 g         2223 t
ORIGIN      
        1 gtccgtcatc gagcagggca agttcgagga ggacttcggc ggcggggtca gccaatgggc
       61 gaccacgacg ttcaacgccg cgttcttcgc cggcctcgac atccccgagc acaaggccca
      121 ctcgatctac atcagccgct atcccttcgg ccgcgaggca acgctcgcct acccgtcggt
      181 cgatctcaag atcaagaaca acacgcccta cggcgtcgtg atctacccga catacacgaa
      241 cacgtcgatc acggtgcagc tgtggtcgac ccggttcgcg gccggagcgc agaccgcgat
      301 cagcaagacg tcagggtgtg gcgacgtcac gctcacccgc acgcgtgtgt tcgtcgacgg
      361 ccacaccgac accgacaagt tcaaggcgaa ctacaactgc aacccgccgg aacactgagt
      421 ctcaccgctt ctgtcctgca gttcggcccc tccaggggcg attcggcagg acagaaccgg
      481 tggccgtcga ggatcgtctg gggactttcc gaacttccgt cgtgcaggcc tgaccagccc
      541 gcgtacgatc cgtagatcca tcgtccggtg acgggcatca gggcgggaca ctccaggggg
      601 cactcgatga acgacgacat gctgtacgag gatcgcatga gcgatgccga cgcactcatg
      661 tggaacatcg agaaggaccc ggcgctccgc tccacgatca cggccgtgtt cacgttcgac
      721 cgggccatcg accgcgacat cctcacccac cgcttcgaac ggctcagccg ggtcatcccc
      781 cgcctgcgcc agcgggtgcg ttccaacccg ctgtcggcgg ccccgccgcg ttgggagacc
      841 gacccctact tcgatctcgg gttccacctg tggtgggtca aggcgcccgg ccgcgccacg
      901 atgcgcgacc tgttgtcgat cgccgagccg gtggcgatgt cgggcttcga tcgggcccgt
      961 ccgctgtggc gcgccgttgt cgtcgagggc ctgcccggca agaagtcggc gctcatcatg
     1021 aaagtgcacc acgcgatcac cgacggcgtg ggtggcatgc ggctgcagct cgaactgctc
     1081 gacatggaac ccgatgcccc cgagcgggag atgccgccgg agccgacgat ccacgtgatg
     1141 agccagcccg agcgcttcgc cgacgcgctc gaccaccaga cccgacacca gctcggtctg
     1201 gcccgccggc tcgccaacgg ggtgctcggg agcgccgcct cggcgctgac cgacccgatc
     1261 ggcacgctcg ccgccggcag cgaactggcg tcgtcggtgg gtcgggtcct gcgccccgcc
     1321 agccatccca tgagtccgct gatggtcggg cgatccctgt cgtcgcggtt cgacacgatc
     1381 accgtgcccc tcgacggcgc caaggccgcc gccaaggtgg ccggcggcaa gctcaacgac
     1441 gccttcgtcg gcggcgtggc ccgggggttg tacaagtacc acctggccca cgggatcgac
     1501 tgcgacgagc tgcgcatggc catcccgatc aatgtccgca ctgccgagct cgagaacgtc
     1561 gccggcaacg ccttcgtccc ggcccgcctc gaactgccca tcgacgccga ggatcccgtc
     1621 gattcgatgc gccaggtcca ctcgatggtc gacggagccc gcatggagcc ggccaacgac
     1681 ctcgtcgaac ccgtcgctag catcctcaac cggctgccca cgacggcggt gacggcgctg
     1741 ttcggttcga tggtcaaggc ggtcgacttc acgacgtcga acgtgcccgg tgccccgttt
     1801 cccgtctact tgggcggagc gaagatggag gcacagttcc ccttcggacc gcttgccggc
     1861 gccgcgctca acatcacgct gctcagctac cagaacgacc tcaacatcgg catcgcgtcc
     1921 gaccccgccg ccgtccccga tccggacgtg ctcgtgaggt cgttgcaggc cgggttcgac
     1981 gagatcctgg cggtcgcctc caagaagtag cctccacggg gtgagcgacg ttgatgtgct
     2041 gatcgtgggt gccggtccgg ccggcaccgc tgcggccatc acgctcaccc ggtcgggccg
     2101 ctcggtgctg gtcgtcgaca aggcgtcgtt cccccgcgac aagatctgcg gcgacggcct
     2161 cacgaccagt gcgctccgcc acctcgaggg gctcggcgtc gagccgcacc agatcgattc
     2221 gtggttcccg gtccacgacg tggtcgtccg cgctccgtac ggggtcgaac gccggttctc
     2281 gctcccccgc ggccagggcc tctacgccgt cgtggcgacc cgccgcgacc tcgacgctgc
     2341 cctcgtcgat cgtgcccgca aggagggcgc cgagatcgcg gaaggcgcgg cgctgacatc
     2401 catcgccaac acggccgacg gcgtcgtcgc cgaggtcgaa ggactgggcg aggtgcgggc
     2461 ccgcttcgcc gtgggggccg acggcatgtg gtcgccgacc cgcaagctgc tcggcgccgg
     2521 ggtcgagggg taccgcggtg agtggcacgc gttccgccag tacgtggggg gcgtcagcgg
     2581 acgggccgca atcgatctcg tggtgttctt cgagccggac ttcctgccgg gctacttctg
     2641 gtcgttcccg ctcccgggtg ggcgcgccaa catcggcttc ggtatccaac gcggtggcac
     2701 acactcgatc caggacatga aagggctctg gcaggacctc ctccagcgtc cacacgtgcg
     2761 cgagctcctc gggcccgacg tcgagcccct cgaaccgcac cgggcctggc cgatcccgtg
     2821 ccgccccggt gagctgtcga cctcggccgg ctcggtcctg ttcgtcggag atgccgtcgg
     2881 cgcctgcgac gtgatgaccg gcgagggcat cggacaggcc ctccagaccg gtgagatggc
     2941 cgcccgccac atcaacgcct cctggcacga tccggcgctc gtcgcgtcgg gttacgaggc
     3001 cgagttcctg accgagctcg gcaacgatgc cgccatgagc agcctgctcg tgcgagcgct
     3061 gcagcatcgc aagggcgctg cggcggcgtt ggcggtcgcc ggcacgagcg actggacccg
     3121 tcgcaacttc atccgttggc tgttcgagga ctcaccccgc ggcatcatgt accgcccgtc
     3181 gctgtggcgc cgcggcgccc tcagcggccc cggcgcctac cgaacgtaac gtcgacgacc
     3241 gagcggagcc cacgggtggg cgtcggtcac gtcgccgggg tgcggtagct gaatcgctcg
     3301 aactcgtagg tgatctcgcg gtcccgctcg accacctcgg tcgccttcag gggtcgaccc
     3361 tgtgcatcga tcgtgatcgt gagcgaccac gggccgtcgg acaccgttcc caccgtcacg
     3421 tcgccattcc gctggtagac gagcttgagg tcggtggggt tgttcgaggc cggatcgcgc
     3481 ttgatgccga gcaccgtgcc agcggcctgt ggcacgggtc gagctccggc ctcgacgtcc
     3541 ttctcatctg cccacacgcc atcatgccgc tgcatctggc cggaccgagc gagttcctgg
     3601 acggcggcac ccgccgcgtc cggcccgagt tcgtcggaga gcctcgcctg ttcgtcggcg
     3661 tcggggacga catcagcgac gtcgtagtag gcgtcgggag agatacggcg gtagctcccc
     3721 ggccgtcgga cctcgtcggt gacgtcgaag tcgtcgccga cctccttggt ggacgtgacg
     3781 tcgtagttct ccgagtcgac gaagtgaacc tcgtggcgaa gctgcttcgt gacctcgccg
     3841 gcggagcggt gggtctcgac gatctcggcg tcgtacgggc gctccaagtc gaaccccgag
     3901 ggaggcgagg agacctgtga gccggccgag cccgtgaacg cgatgccggc ccaggccgcg
     3961 acaccggcga ctccagtggc gatgaggagc atggtcctgg atgacatgtc cacctcacac
     4021 gatctcgagc atcagcaagc ccctcgtcga acctcagcgc aggtgaccga gccgctacgg
     4081 aaacagcggc gggccgaaga tcttcttgtc gggatcggtg tgacgaccct tgcgcgacat
     4141 cgggccgaac cgcggcatct cgctgaacgg gatcttccac ttgatgaacg gcccgatctg
     4201 caggtagttc atcgtctgcg cgatctggaa ccccaccgag tggtgccaca ggatcgacgg
     4261 cacgttgtcg taggcgacga acccgtagcc gttgccaccg gcacgggggc tggtctcggg
     4321 agggtcgcgc cggaagaaga agtcggccat cgtgttggcc agaccgcccc ggcggtactc
     4381 acggtcgacc cacaggtcga acatcaagaa ctcgtcagcg gcgagcttca tgcggggcac
     4441 gccgttggcg agcgccgtct cggttcgcac cgtctcccag atccgaccga ccggttccat
     4501 gtcctcgttg agcgccacga tcacgttcca acgaccgtca cgcatcgcct gctgagcgat
     4561 cttcagctgc cgcttgctcg acgtgatctt cggcaggatc ggatcgtcgg gcgtgttgac
     4621 ctcgacgatc gtgaacttgg ggtcgatgtc gtcggggtcg atcggcgacg cccgcggcgt
     4681 cacgtagacc acgaaggccg acaccaccgt cggcttgtac tcgttgagct tccgcttcag
     4741 tccctcgatc gatgccattc ggggaagcgt acgtgctcga tcggtgttct gcgcgcccaa
     4801 acccgacgtt gggctcatgt tctcaacgga accgggcgag caccttctga ccaccttcgc
     4861 cgagctcgac gagcgtggcc cgatccccga tcgccagccc ggcgaacccg cccaccagcc
     4921 cagcgaagaa caccgcgaag agcgaacccc agccggcatc gagcgtcagg cggaccggcc
     4981 acgcgaacgc accgataccg atcacgagcg ccgcggcggg gcgcagggcg atcagcgagt
     5041 ccttccacgt cgtgccgagc agacggttcg cgacctggat tcggaacgcc gagtacggga
     5101 tgatcaccgc gacgtgcacg gcggcgatcg cgatcacgcc ccgatcgacc acgagcagga
     5161 acccacccat caggatcggc acgaagatca gcgagaagta gagaccgagc cggggtttgc
     5221 ccatcgcgtt gaacaggtca cccgacgcat agccgacacc gacgaacccg ccggccacgc
     5281 agaggagctg catgatcggg atggcgccga ggacctcggg atagacgacg gtgatgaagt
     5341 cgcgggcgat cagcgacatt cccacgccgg cggcgaaccc gaacatgcac agcaaccgga
     5401 gcgacttgag ggaggcgttg cgcagcgcct ccggtccggc ctcacgggcg gcggcgtagg
     5461 ccgggaacgc cacgttcgag aagaggttgt agacgctgcc gagcacgagc tcgggcagcc
     5521 ggaacgccgt gtagtactcg ccgaactgtc gggacttgct gttgaagtgg cgtgacagca
     5581 ccaggtagtc gccctggagc cacagcatcc ccaccagctg cgcggcgaac atcgccgatc
     5641 cgaacctgag catctgaccg gcgatcttga ggtcgaagcg gagggttggc ttgaaacgca
     5701 ccagcgccca ggtgacgacc gtggcggcga tctcggcgct gagcacaccg atgaccatgg
     5761 cgatcggccc gacgccggcc ttcaacaggc tgaccgagat gacgaatcgg cccagcgaac
     5821 gcgtgaagtc ggccttggcg cggcgcttga agtcgagatc gcgcttgagc atcgcgtcgg
     5881 gcacctgccc gaggcccttg acgagcacca acgcggccac gacccggaac aggttggggt
     5941 tgcccgcccc caagaagtcg tcgatggcac cggcaccgaa gaagatggcg accgacaaca
     6001 cgacggccac catcatgttg acggtgaacg ccacggccac ccgctcggtc tgacccttct
     6061 cctgctcgaa gatgagcgag gcacccatgc cgacgccgag gaggatgtcg atccagaaga
     6121 ccaacgtgag ggcgatgccg accacgccga tgccgtcggc tccgaggacc ttggtggcga
     6181 tggtgtaggt ggcgaacgag ccgacctgca tgagcgcgaa gccgaccagc gcccagacga
     6241 aaccgccggc ggccttgcga ccgaggctga gatcgtcgtc gcccgacgtc ccgtcgaccg
     6301 tcccgtcggc cccactctcc gctacgtctg ccaccagcgc ccctgatcgt gtcctccggc
     6361 aggcgggccg ggatcgtcca tggtaccgac ccgctcatcc gcagcgcgaa caggcctccc
     6421 ccgctactgt gcgccgcatg cgcacacgtc tcaccgagat cctccagatc gaacaccccg
     6481 tgatgctcgc cggcatgggc ggcgtctcct acagcgagct cgccgcggcg gtctccaacg
     6541 gcgggggctt cggctgcctc ggggcgtcca ccatgtccaa cgaccgtatg gaacaggaga
     6601 tcgccgccac caaggcgctc accgaccgac cgttcggcgt cgacctcctc accgccgccc
     6661 ccggcgacat ggaggcgcag gtcgagctca tcgttgccgg cggtgcgacc gtgttcgctg
     6721 ccggcctcgg cgttcccagc aaggtcatcg accagtgcca cgacaacaac atcatcgtgg
     6781 tgaacatctg cggcaaggtc cgccacgcga tcgctgccgt cgaggcgggc tgtgacatcg
     6841 tcgtcgccca gggcaccgag gcgggcggcc acaccggcca ggtcgccacc atggcgctcg
     6901 tgccccaggt cgtcgacgcc gtcggcgatc gggttcccgt ggtcgcggcc ggtggtctgt
     6961 acgacggccg aggcctggcc gcagcgctcg cgctcggcgc cgacggcgtg tgggtcggca
     7021 cccgcttcat cgccacgccc gaggcctacg gcgtccgggg ttacaaggat ctcctgctcg
     7081 gcgccaagga ggactccacg gtcatcagcc ggggcttcac cggcaagacc tgccgagtgc
     7141 tgcgcaacga ctacacccag tactgggagg agcaccccga cgagctgcag ccgttccccc
     7201 agcagttcat ccgttcgatg aacgacgggg cgaaccacct cggtctgggc ctcgacgacg
     7261 accgcgtcga ccccaacaag gagttcttcc cggcggggca gggcgttggc ggcatcaacg
     7321 agctcgtgcc cgcggccgaa ctggtgcagc gcttcgtcac cgaggccgag gagatcctcg
     7381 accgggtggc gtcattcgcc cggaactgac ccacacacgt tttgccctgc agttcgatcg
     7441 gtttgccgat tgaactgcag ggcaaacgag caggggcggc tgcccggtcg aagggctaga
     7501 gccagccctt gaccttctcg atcagggcag gctgatcggc gtcgaagggc gtcacgttga
     7561 ggacggtgac accggcctcc ttgtacgccg cgatgcgctc cttcacgtag ccctcggggc
     7621 cgaccaggtt ggtgtgctcg agcagctcgg tggggatggc cgctgcggcc tcctccttct
     7681 tgccggcgag gtacagctcc tggatctgct tggcctcctc ctcgtacccg taccgggtga
     7741 tgagggcgtt gtagaagttc tgaccgaccg cgcccatgcc gccgacgtac agcgccatga
     7801 ggggacgggc catgtcgagc acgcccttca catcgtcgcc cacggcgagg agaccaccgg
     7861 cgacgacgtc catgggcccg agctcaggtg aacgcttggc gtagcccttg tcgagcgacg
     7921 gcccgaacgc atccttcgcc ttctccgggt agtagaagat cggcaaccag ccgttggcca
     7981 gctcggcggt cagctcgagg ttcttggggc cgagcgacgc gatgtggatc gggatgtcgg
     8041 accgcttcgg atggttgatg atcttgagcg gcttgcccag gcccgtgccc tgctcgggcg
     8101 ggagcggcaa ctggtagtac ttgccgtcgt aggtgagctt ctcttcacgg gcccagacct
     8161 tgcggcagat ctcgatgatc tcccgcgtac ggccgagcgg ggcgtcgtac ttcacaccat
     8221 ggaagccctc gatcacctgg gggccggacg cgccgaggcc catgatgcaa cggccgtcgg
     8281 acacgtggtc gaggcccgcc gcggtcatcg cgaggagcgt gggggtccgg gtgtagatgg
     8341 gcaggatgcc ggccgcgatc tgcaccgtct cggtgcgggc agccaggaag cccatggcgg
     8401 acacggcgtc gaagctgtac gcctcggcga cgtagacgat gtcgagcccg gccttctcga
     8461 gcgccacggt ctgctggacc gactgctcga accctccggc gtatggcatc tgcattgaaa
     8521 tgcgcatgac tagttgttct ccccctcgat gaccttgccg tgcttgtcga tgcgctgcag
     8581 cgtgccacgg ttgagcgacc cgacgtcggt cccgttggga ccaccgatct cgaagagcac
     8641 ccggaaccgc tgccaatcgc ggccgtcctt gtggtcccat ccgctggtca ggatgacctt
     8701 gccgggggtg ccggcgggca cgcggggcag gtcgaccttg ctgatcaccc gatcgtggag
     8761 gagcagctcg ttaccggttc gtttcgccat gggcgcttac cttaccgatc ggccgcgcct
     8821 gcgagggaag tcgtgacgac cgttccgacg acaacgtcac gtcgcggggg cgaggctccc
     8881 ccatcgacgc cgcactcgcc acgtcgatga gcgactcgtc gaggccgaag cgacgacaca
     8941 ggatgcggcc gacatcgacg cggctgaggc gctcggggcc cacgaggtgc cagacaccgg
     9001 agcgctcgcc cgccggcagg ctgacgatgt cccacagctg ggaggccagg tcgtcgacga
     9061 ggatcggggc gcggaattcg tcgtcgaaca acgtgacccg ctcccccgaa cgaagccgtt
     9121 cgacgaccca ggccgacgtg ctgtcggtgc cgtcgggggc gaggatcaac gacgtccgga
     9181 cgatggccgc atcgggacac gcctcgcgca ccgcggcctc ggcgagcgcc ttggcccgcc
     9241 cataggcatg gatcggcgcc ggttccgccg actcggcgta cggcgcgtgg tcaccgtcga
     9301 acaacgcgtc ggtcgacacg tggaccaacg cgatgtcgag cgcagcgcat gctgcggcga
     9361 cctcggtcgt ggagtcgacg gtgaggcgct ggtcggtctt cgagtacgcc gtgtggatca
     9421 cgacgtcggg ccggcggcgc tccacgaggt cgaacgccgc caagggctgg gccaggtcga
     9481 tgcgatgcgc cttcaacccg gcagatgccg gccgctcacg gatcgtgacc tcggtcgacc
     9541 agccggccgg tgtgcaccga gccagttccg atccgaggag tccggcaccg ccggtgatca
     9601 acgctctcat cgacggctca gcgtcggctc tcggtccacg gccgaccctc gtggggatcg
     9661 atgtcgtgtg ggagccccag cacccgctcg gcgaggatgt tccgctgcac ctgcgtcgtg
     9721 ccgccaccga tcgtcagcgc tcgggcgaac aggtagccga agtgccacat gtcgaccggc
     9781 gctcccatcg ggccctcgcc ggtcagcatg gcgccggtcc cgaccaggtc cttcgcgagt
     9841 cccatgatct tctgcccgtg ctcgtcggcg agcagcttgc ggatcgacgc ctcgggaccc
     9901 ggggcctcac ccttgatggc tgcgctcacg gtgcggagcc ggatgagccg caggatctcc
     9961 gcctcgatgt acagcgacgc caactcctga cgacgcaccg ggtcggtgac accgccgcgc
    10021 tcacggacga tgtcgaacaa tgcgtacgcg tcggggccca tgccccacaa cgcgccgccg
    10081 cccgacagcg agacgcgctc gttgccgagc gtcaccttcg cgaggcccca tccccggttc
    10141 acgtcgccga cgatcgcatc ggcgggcagc cggacgtcgg tgaagaacac ctcgttgaag
    10201 agggcgacgc ccgtcatctc gcgtaccggc cggacctcga cgccgggcgt gtccatcgga
    10261 cacacgaagt aggagatgcc ctggtgcttg ggggcgtcgg ggtcggtgcg ggcgatcagg
    10321 atgccgaact ggctgttctg cgcacccgag ttccagatct tcgagccgtt cacgacccac
    10381 tcgtcgccat cgcggacggc ccgtgtgccg aggctggcca ggtcgcttcc ggcgtcgggc
    10441 tcgctgaaga gctggcacca gatctcctcg ccggccagca gcgggaacag gtagcgatcc
    10501 ttctgctccg gggtgccggc gtgcaccagg gtcggccccg cccagccgat cccgatcatg
    10561 ttgtcggggc gacggatccg ggcgcgcttg agctcgtcgt cgatgatcag ctgatggatc
    10621 gggtcggcgt cgagccccca cggccgcggc cagtgcggcg cgacgaggcc ggcctcggcc
    10681 agctcccgac cggtcggtga cggatgatcg gcgatccagg cgcgcacctc gatgcggcgc
    10741 ggatcgtcct cggacggcat ctcgagatcc acggcgtcac cgtaggcgct gcagccggtc
    10801 cacggcggag gcgacgacct cgctccgctt gcagaacgcc catcggacga acgtccgacc
    10861 ccgatcggcg tcgtcgtaga acacgctgct cgggatggcg accagccccg ccgttgcggg
    10921 gagctcgagg cagaacgtcc gggcgtcggt gtggccgaga tcggcgacgt cggtcgtgag
    10981 gaagtacccg ccctcggcag tggggacgtc gaaccccgct gcagccagac cgaccgccag
    11041 acgatcccgc tcggagcgga cgcgttcgcg aagtgtggcg aaatggtcgt cgcccagtcg
    11101 cagcccgacc gcgatgccgt actgcagcgg acctccgctc acgtacgtca ggaactgctt
    11161 cgacgtgcga acggcggtga cgagcggctc cggtccgacg atccacccga ccttccagcc
    11221 ggtgcactgg aacgtcttgc cgcccgacga gatggtcagc gtccgctcgg ccataccggg
    11281 cagcgtcgcc agcggaatgt gcacaccgtc gtagacaagg tgctcgtaca cctcgtcggt
    11341 cacgacgagc aggttctgct cgatcgcgac ggcggcgatc tgctccgact cggcctgggt
    11401 caggaccttg ccggtcggat tgtgcggcgt gttgatcaac agcagcttgg tacgaggggt
    11461 gatcgccgcc cgcagcacgt cgggatcgaa cccccacgcg ccgtcgacca gcaccaacgg
    11521 caccacccgg cggacgccgc cggccatcgc caccgccgcc gcgtacgagt cgaagtacgg
    11581 ctcgaacatg acgacctcgt cgccggggtc gagcagcgag atcatggcgg ccgcgatcgc
    11641 ctcggtggcg cccgtcgtga ccaacacgtc ggtaccggga tcgacgcgca ggtcgtagaa
    11701 ccgctcctgg tggtcggcga tcgcctctct caacggcagg atgcccggcc cgggcgggta
    11761 ctggttgtga ccgtcgcgga tcgcggccac ggcggcgtcg gcgacctccg ggggcgggtc
    11821 ggtgtcgggg aacccctgac cgaggttgat ggccccggtc tcgttggcca acgcggtcat
    11881 ctcggcgaag atcgtcgttc cgaagccgcg caaccggcgg gcgagcgggt ccggattcac
    11941 gggacctgag gctaccgacc caattcggta cagcacatca ccggtacggc cgaagtgttc
    12001 gggtacctcg gcccactcgg gtcgatgcct cgctcccgaa aggtcctcgt gaaccgccga
    12061 acactcgcct cgatcgccct cgctgccgcc tgtgtcgtcg gcctgaccgc ctgcgacatc
    12121 cacagccccg acatggagct cggcttcacg gccaaccccg acggcacgac aacggtcacg
    12181 ggcctggccg ccgatcagga ccaggcgggc accaagctgc tggtcgtcgt cacggtcgac
    12241 ggcacgacga acaacgccgt ggctgccaac ctcccggccg ccgcgtggtc gccgcaacgc
    12301 cccttcaagt gcgggaacaa ggacaaggac ggcaacgagc agacctgttt cctcaccgtc
    12361 cggctcggcg aggaccatcg cttcacggcc acgttcacca acccgacgga tcccgagaag
    12421 ttggtctgca tcactccgct cggcgtcgcc gtgaagacgc cgactgggga ggggctggtc
    12481 actcagcaca ccaagcagtg cttcacggcc gcgaagatcg ccgagctgac tggcaccacc
    12541 cccacgacag cgccgcccac acccgtgcca cccacgacag ctgcccccac caccagcgcc
    12601 gcacccacca cgaccactga ggcgaccacc acgacgacca tgccggcccc cacgtccacg
    12661 acctcgacga cgatcttcga tcccggcccc gggcccggcg gtccctgcgg ccccgatccg
    12721 atggatctca gctgcatctg aaccccggcg ccgacggcgt ggcgtgagat gccggccggg
    12781 atcccgatcg cgcccggcct gcgagccctc ggcgccggac ctacactgcg gaccgcaccg
    12841 aacccggggg ttgtacatga gtggtggttg gaacttcgcc gagatctggg agcgcatcgc
    12901 ggacaaattc cccgacgccc aggcacaggt ccagggcgac cgacgtttca cgtggaccga
    12961 gttcgaccgg cgcgccgacg gcatcgcccg gacgctcctc gatcacggcg ccggtcacca
    13021 ggacaaggtg gcgctctacc tctacaacgg tccggagtac atggagacgg cgtttgcctg
    13081 ttacaaggcg tcgctcgtcc cggtgaacac caactaccgc tacatcgatg acgagctcac
    13141 ctacctgtgg gacaacgccg acgcggtcgc cgtcgtgttc cacggcgagt tcacccccac
    13201 catcgagcgc atccgttccc gcgtgcccga cgtcaaggtc tggctgtggg tggacgacgg
    13261 caacggggac tgcccggagt gggcgatccc ctacgaggac gctgccgcga ccgagaccga
    13321 acgcgtcgtc ggcgccaagg gccgctccgg cgacgacatc ctcatgatct acaccggcgg
    13381 caccacgggc atgcccaagg gcgtgatgtg gcgccaggac gatctcatcc acgccacgtg
    13441 caccaccggc ctcccgatcc tcaacggcga acagtccgag gtcggctacg acgcactgct
    13501 cgaggccatc gccgcgcccg gcgcccccgg gctcccggcg tgcccgctca tgcacggcac
    13561 cgggtggttc acggccaaca tcttcctggt gcaggcgggg tcgatggtct ccctcgtcga
    13621 ccgccggttc aacgtcgacg agatcctcga caccgtcgag cgcgagggcg tcggagccat
    13681 gaccatcgtc ggcgactcct tcgccaagcc catcgtcgct gccctcgacg ccaaccccga
    13741 ccgctgggac ctctcgtcac tcgtcctcat caccagctcc ggggtcatgt ggtccgagcc
    13801 ggtcaagcac gccctgctcg cccaccaccc cggcatgttg ctgatcgacg tgttcagcag
    13861 ctcggaagcg atcggtctcg gccagtcgat ctcggccgcc ggcgcggagt cgcacacggc
    13921 gaagttcagc ctcggcccga gcagtcgggt catctccgac gaaggccgcg acgtcgtgcc
    13981 cggctccggc gagaagggac gggtggccgt ccgtgggttc accccgatcg gctactacaa
    14041 ggacccggag aagtcggcgg ccacgttcat cacgatcgac ggcgcccgct attcgatgcc
    14101 cggtgactgg gcgaccgtcg aggaggacgg cacgatcagc ctcctcggcc gcggttcggt
    14161 gtgcatcaac acgggcggcg agaaggtctt ccccgaagag gtcgaggagg cactcaagct
    14221 gcacccgacc gtcaacgacg ccgtggtggt cggggtcccc gacgacaagt tcgggcaggc
    14281 catcaccgcg gtggtcgagc cggcggccgg tgcgtcgatc gacgaagcca ccctcatcgc
    14341 cgaggtcaag gcgcacctcg ctccgtacaa ggcacccaag cgggtcctgg tcgtcgacac
    14401 catcggccgg gcaccgaacg gcaaggtcga ctacaagcgg cacctgacct acgcgctcga
    14461 tcagctcggc atcgaaccga gcccgatccc cgtcgccaac gcctgaccgc ctgaccggct
    14521 gaccggctga ctgccggagc gccggccccg gcgcccggtc gccgggacct cagcgacggc
    14581 ggaggcggcg gagacgcagc aacccgagcc cgacgaacgc ggcgccggtg gccaggagg
//
LOCUS       DAOSOA010000040        14442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4208_length_14442_cov_6.455203, whole genome shotgun sequence.
ACCESSION   DAOSOA010000040 DAOSOA010000000
VERSION     DAOSOA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14442
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4208_length_14442_cov_6.455203"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKY13_04415"
     CDS             <1..606
                     /locus_tag="PKY13_04415"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT79203.1"
                     /translation="RAIAAALGSRFGVGNSTYHHGIEPQEVDHIPFGAYPVAVIRDVG
                     GWDEAITANEDFEFDYRVRQSGRALLFDPRIVIFWQTRQDVGAFFQQYRRYGQGKALV
                     LAKHPNSASPRHLLPAAVVGALAAAVAISPLKPRWALSILAPYVGILAVATVTVSPTL
                     VVSESQRWLPAAFAAMHLGYGHGFWEGAIRRIVSGSRGRVR"
     gene            617..689
                     /locus_tag="PKY13_04420"
     tRNA            617..689
                     /locus_tag="PKY13_04420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:650..652,aa:Gln,seq:ttg)
     gene            735..1844
                     /locus_tag="PKY13_04425"
     CDS             735..1844
                     /locus_tag="PKY13_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT79204.1"
                     /translation="MSDPETQRPLYAVVLAAGEGSRMKSERPKPLHRLCGKPMLAYVL
                     ESLATCHADRAAIVVGHKGEWVTKKIAEENVGVALEFVEQAVQRGTGDAALIGLSGLP
                     DDLDDDSDVIVLPGDTPLLTAATIGRLVEQHRATDVAATVLTAVLDDPAGYGRVVRGR
                     NDRVVRIVEHGDATPEQLEINEINTSIYCFKRSLLAPALRRLNPDNAQGEYYLTDVVA
                     VLAEAGHSVDTVIAPSAAEASGVNDRLQLALAESEMRRRTNEALLRSGVTMVDPASTF
                     VDTTVQVGRDVTLFPGVMLQGSTVVGDGTELGPNTRLVDCRVGANAVIEETTARRAVI
                     GDESHVGPYAVLAPGSEVASGVDTGPFYNSAAPSA"
     gene            1860..2843
                     /locus_tag="PKY13_04430"
     CDS             1860..2843
                     /locus_tag="PKY13_04430"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOT79205.1"
                     /translation="MELITKKRLHLVAGRNNRALAEEVAQRLSVELGHVSLSEFANGE
                     LHCKYGDSIRGADVFIFQGHCSTSDMSINDALMEQLIMVDAARRASAKRITVVAPFYG
                     YGRQDRKAEGREPITAKLVANMFKTAGAKRLVSVDLHSGQIQGFFDGPVDHLTAMPVL
                     VEWMANNLRGELVVVSPDAGRVKVAERYANLLHANIAIVHKRRVRGVKNAVEAKDVVG
                     EVEGKTCVLIDDMIDTGGTLVAAAEQLAERGAASVYAAATHGVFSGPAIDRIKNSVLE
                     KVVITNTLPLPPEKQIDKIEVLSAAGIIADAIDAVFEDTSVSDIFDGQNQH"
     assembly_gap    2845..2944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3158..3778
                     /locus_tag="PKY13_04435"
     CDS             3158..3778
                     /locus_tag="PKY13_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOT79206.1"
                     /translation="MAEVTLTATTGRPLGSSASRRLRAEGKIPATVYGMDKDAVSVTI
                     TRSDLRKAMTTDAGVNALIKLEIDGAATEYALVKEIQRHTVRREVTHLDLLRIDPETA
                     MVLDVPIVLTGDAKKVSGGGGFVDQKLSTLRVTVRPDSIPTQLTADISAMDVEDVLTI
                     ADLTLPRGVTTELDPELPIVTAALTRAAIVAARAAAKAEEAAAAGA"
     gene            3803..4438
                     /gene="pth"
                     /locus_tag="PKY13_04440"
     CDS             3803..4438
                     /gene="pth"
                     /locus_tag="PKY13_04440"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT79207.1"
                     /translation="MRRGARSLGAASSAKQHNNTPWDLLVVGLGNPGPKYRGTLHNVG
                     VEVLDLLAERHRERFRSSKEHALVAELRIGAARVALAFPQTYMNESGRSVGPLVKRYG
                     IDDLTKLVVVHDELDLDPGRLKVKLGGGLAGHNGLRSIKAHLHSDEFARIRIGVGKPP
                     GGKERGVDHVLAPPSKRDRELIAVCVEEAADAVEMMLSDGIDATMARYNQR"
     gene            4435..7908
                     /gene="mfd"
                     /locus_tag="PKY13_04445"
     CDS             4435..7908
                     /gene="mfd"
                     /locus_tag="PKY13_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT79208.1"
                     /translation="MTDVAESRVAAVAPLRSLPGLLREEPALLGTLGRSSATLAVPEP
                     ARAVTIAAIATLSKRSPIVVAVPTSADAERLAGDLACFLGADEVDVFPAWETLPFERV
                     SPSVETMGRRMRTMWHLRDPERSPKVLVAPARALVQRLGPHVEDVEPIVITPGAIVDQ
                     ADLVERLVEAGYRREYQVEHRGEIAVRGSIVDVFPSTADGPVRIDLWGDEVERLTDFS
                     VNDQRSDNDVERVEIFPCRELMTTDDVRERAEALIESEPWGRDQWEKLADGQSFDGME
                     SWLPWLTGDGATTAHVLFDLVGPESQVILVDPRRMRDRAADIIAEEVDLARTLSGTWG
                     ASDDGVLPRLHLDFERLLTRTTAPTWTMTTAPEGPGIETVAAAAWFQASGDGSRLVNQ
                     LRDLSASGYRIVVAAEGAGSAERVDRLLNEQGVSYRLRPEGGAALAEPGGHITVAALN
                     RGFILPSVKLAVLSESDLTGRRRTHRPARQRKRQAQGFFDDLKVGSYVVHQHHGVARF
                     GGMVKRTIGGADRDYLLLEYRGDDKLYVPSDQIDAVRHYTGGDSPSLSKMGGADFART
                     KAKVASEVATIAQELVVLYQKRVHAKGHAFAEDSPWQKEMEDSFPFAETPDQLQAIED
                     VKHDMEAERPMDRLVIGDVGFGKTEVAIRAAFKAVQDGYQVAILVPTTLLAQQHYATF
                     SDRFAPYPVRVEVMSRFLTAKQSRVVADGLKSGEVDVVVGTHRLLSGDVEFKKLGLLV
                     IDEEQRFGVSHKEAIKQFKADVDVLTLTATPIPRTMEMSLTGIRDLSLLQTAPSERQP
                     ILTYVGEYDDRAVAESIRRELLREGQVFFVHNRIADIEEKAADLRDLVPEARIAVAHG
                     QMDEGSLEQIVVDFWEGRYDVLVCTTIIESGIDMPTVNTLVVERADLLGLGQLHQLRG
                     RVGRAGMRAYAYLFFPPERELSEEAYERLRTIGEATELGSGFRIAMRDLEIRGAGNLL
                     GTGQTGHIAAVGYDLYCSMVNEAINDLNGETKAEDVEIKIELPLDANLPADYVEREDL
                     RLDAYRRLAEVKSFTDVDDIAAEWVDRYGPVPDAAGALLDIARVRAECVRTGVTDISV
                     TKSTGFGGPPLVARISPLKLPQSKQMRLERLYKGAVYKNDSQLQLPIKAGRAMIATLL
                     EAFTQMVPVDE"
     gene            7967..9010
                     /locus_tag="PKY13_04450"
     CDS             7967..9010
                     /locus_tag="PKY13_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79209.1"
                     /translation="MKRLLVSSIAAVVLLTACGVAEDTTAATVNGQDISVSAVTKLAK
                     SKYIVDTASAGGLVSSKDVVVGSTTRRAALGLLVQSEVYEAGIAARKGEITDVDRDAA
                     EAQISDYESNNQTTIDDTARPVIVRLIAAQAALGRILGGADIESAASPEKIEAYFDEH
                     ADEFAKICVDGFAVTSDQAAAAQAAVDRGDDIAAILADSSLQAQSLSQTGTETCVLSG
                     QVSDSPLADLITTGPVGQWSSTDISDATAGDLTVFVRPNSRDEPTLDDPDISDQIAQQ
                     LQAEATSAAQEAFGAEAQTILERADVEIDSRYGRWDPSDPSLILAPLAPTPSAKAAAE
                     ALSADDLAGLQGQ"
     assembly_gap    9075..9174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9175..9993
                     /locus_tag="PKY13_04455"
     CDS             <9175..9993
                     /locus_tag="PKY13_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HOT79210.1"
                     /translation="LSDVKLSIEEWPSEPVTVIQRLGLPDERIFEVEWADLDRGVEAD
                     HLTSIYIPTLEQPVAAELVQFDELVHALRTGCPWDREQTHESLKRYLLEECYELLEAI
                     DGLAADTDESIDALQEELGDVLFQVFFHATIAAESGWFTLADVARTVHDKLRDRHPHV
                     FGSATFESAEALAANWEAQKKTEKSRDSVMDGIPPELPALLYALKVQKKAAASDYREP
                     VAEFVAALDAGTMDAAALGELLFGVVQVARTAGLDPEDELRQASRRFVERFESR"
     gene            complement(10004..10969)
                     /gene="speB"
                     /locus_tag="PKY13_04460"
     CDS             complement(10004..10969)
                     /gene="speB"
                     /locus_tag="PKY13_04460"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF002564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOT79211.1"
                     /translation="MEANHDPSVSGDVGDAQGFDMTWAGQLSFGRLPATRDLTGVDIA
                     VVGIPYDNAVTNRPGARFGPRAIREQTTLSAEFPEGLWPWDHSVFDRHKVIDYFDIGY
                     RPGVPEEMVTNVVDHVGRIIDAGASVLGLGGDHFVAYPLLLATAERHGQLSLIHFDAH
                     SDTWDAGDELNHGTMFLRAARDGILDPATSVQTGMRTPNVSHGFNVIHADRLFDIGIA
                     ATIAEIREIVGDRPAYLTFDIDFLDPAYAPGTGTPVIGGPTTWQARQLLFGLAGLNIV
                     GADLVEVAPIYDPSGQITALAGATIAHDELYLLGLARDRRAGVSR"
     gene            11074..11979
                     /locus_tag="PKY13_04465"
     CDS             11074..11979
                     /locus_tag="PKY13_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT79212.1"
                     /translation="MLQVEDVRWRYGDKIVLDGIDLEVPAGKVIGLVGPNGAGKTTLM
                     RILFGVLTPDTGSVRFDGRPADEEARRSWGYMPQERGLYRDMRVLDQLVWIARLHGID
                     RSTARRRAVDLLERLGLADRRDDKVEALSGGMAQRVQLAAAMVHEPDVLVLDEPFAGL
                     DPVAVQFLSDVITDHAAAGRHLLFSSHQLDLVEELCETVTMMHRGKVVLGGDVRQLKA
                     DSPDRTLRVDVEVEHSWVAPLGVEVTAAGAAGSRLRLVPGVRAGEVLDAVRAHHEVTD
                     FGVEAPSLAELFLAAAGDVTAGALR"
     gene            11976..13136
                     /locus_tag="PKY13_04470"
     CDS             11976..13136
                     /locus_tag="PKY13_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT79213.1"
                     /translation="MTAVGATRLVAAREIRETTRRKSFWIVLVVLALAATAAVVVPTL
                     LDDDGTRYRVAVVGDRPGLAEALTTSLAGLDATVRTSLVTTATDAERRVEDGSADLAI
                     VTGDRARLIVKAGEHQELVGAASQALSTIGFAADLEAEGVDAGRITDLLSRPAPTVTE
                     IDRGAADRKGASFAITLVLYILLLTLMVQAANGTAIEKSNRISEVLLAIVRPGPLLFG
                     KVIGISVTGLLTFAAGGIPIVISLAIGSDLPPGIGGALAGGAIWFVLGMVLYLTLAGS
                     LGSLAERQEEAGVVVAPLTFLLVGTFIAAQSAADTTFGIVLALVPLTSPLVMPARIAE
                     GVATVPEMAASALLLVAAIALVARFGAVVYGRAIVRTGRRLKLKDVLRSTPA"
     gene            13218..>14442
                     /gene="eno"
                     /locus_tag="PKY13_04475"
     CDS             13218..>14442
                     /gene="eno"
                     /locus_tag="PKY13_04475"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT79214.1"
                     /translation="MSIIEHIHGRQIIDSRGNPTVEVEVVLTTGAAGRAAVPSGASTG
                     QFEAAELRDGGSDYLGKAVTNAVDHVNDEIAEHIVGLDALDQRQIDRILVDLDGTPNK
                     SRLGANAILGVSLALARAAAEEVGLPLFRYVGGANAHVLPVPMMNVLNGGEHADNNVD
                     VQEFMIMPVGAASFSEAMRWGIETYHVLKKVLHDKGLSTAIGDEGGFAPNLGSNEEAV
                     QLLCSAIEQAGYTPGEQIAIALDAASSEFYKDGKYVLAGEGRSLDSAEFAGYLGELCS
                     KYPIVSIEDGMDEEDWEGWSLLTKQIGGSVQLVGDDLFVTNVARLSRGIEVGVANSIL
                     VKVNQIGTLTETLQSVELAHRNAYTAVMSHRSGETEDTTIADLAVATNCGQIKTGAPA
                     RSDRVAKYNQLLRIEE"
BASE COUNT         2242 a         4899 c         4847 g         2254 t
ORIGIN      
        1 cgggccatcg ctgcggcgct cggttcgcgg ttcggtgtcg gcaactcgac gtaccaccac
       61 ggcatcgaac cccaagaggt cgaccacatt cccttcggtg cgtaccccgt cgccgtcatt
      121 cgcgacgtgg gtggctggga cgaggcgatc accgccaacg aggacttcga gttcgactac
      181 cgggtgcggc agtcgggtcg ggcgctcctg ttcgaccctc gcatcgtcat cttctggcag
      241 acgcgccagg acgtcggcgc gttcttccag cagtatcgcc gctacgggca gggcaaggcg
      301 ctcgtcttgg ccaagcatcc caactcggcg tcgccgcgac acctgttgcc cgccgctgtc
      361 gtcggcgcgc tcgccgctgc ggtggcgatc tccccgctca agccgcgttg ggccctctcg
      421 atcctcgctc cgtacgtcgg catcctggcc gtagcgaccg tcaccgtctc gcccacgctc
      481 gtggtgtccg agtcccaacg gtggttgccg gcggcgttcg ccgcgatgca cctcggctac
      541 ggccacggct tctgggaagg tgcgatccgt cgcatcgtct cggggtcgcg tgggcgggta
      601 cggtagggcc cgtccgtcgc gggtagttca attggtagaa caccggactt tgaatccggt
      661 tgttgtgggt tcgagtcccg ccccgcgaac ccgttggaga tacccgtgaa acgcagtgag
      721 gatgatcagc cgacgtgagc gaccctgaga cccaacgccc cctttacgcc gtcgtgttgg
      781 cggccggcga gggttcccgc atgaagtcgg agcggccgaa gccgctccac cggttgtgcg
      841 gcaagccgat gctggcgtac gtgctcgagt cgctggccac ctgccacgcc gaccgcgcgg
      901 ccatcgtggt cggccacaag ggcgagtggg tcacgaagaa gattgccgag gagaacgtcg
      961 gcgtcgcgtt ggagttcgtc gagcaggcgg tgcaacgcgg caccggcgac gccgccctca
     1021 tcggcttgtc tgggctcccc gacgacctcg acgacgacag tgacgtcatc gtcctgccgg
     1081 gcgacacgcc gttgctcacg gccgccacca tcggccggtt ggtcgagcag catcgggcga
     1141 ccgacgtggc ggcgacggtc ctcacggcgg tgctcgacga tcccgctggc tacggccggg
     1201 tggtccgtgg gcgcaacgat cgtgtggttc gtatcgtcga acacggggac gccactcccg
     1261 agcagctcga gatcaacgag atcaacacgt cgatctactg cttcaagcgc tcgttgctcg
     1321 cgccggcact gcgtcgcctg aatcccgaca acgcgcaggg cgagtactac ctgaccgacg
     1381 tcgtggcagt gctggctgag gccggccact cggtcgacac cgtcatcgcc ccgtccgccg
     1441 ccgaggcgtc gggcgtcaac gaccgcctcc agctcgccct cgccgagtcg gagatgcggc
     1501 gccgcaccaa cgaggccctg ctgcgttcgg gcgtcaccat ggtcgatccg gccagcacgt
     1561 tcgtcgacac caccgtgcag gtcggccgcg acgtcacgct cttccccggc gtgatgctgc
     1621 agggctcgac cgtcgtcggc gacggcacgg agctggggcc gaacactcgg ctcgtcgact
     1681 gccgcgtcgg tgccaacgcc gtcatcgagg agacgacggc ccggcgggcc gtcatcggcg
     1741 acgaatctca cgtcggcccg tacgccgtcc tcgctcccgg cagcgaggtg gcgtccggcg
     1801 tcgacaccgg accgttctac aattcggccg cgccgtcggc ctgaagggga agccagctca
     1861 tggagctcat caccaagaag cgcctgcacc tcgtggcagg ccgcaacaac cgagccctgg
     1921 ccgaggaggt ggcgcagcgc ctcagcgtcg aactcgggca cgtgagcctc tccgagttcg
     1981 ccaacggcga actccactgc aagtacggcg actcgatccg tggcgccgac gtcttcatct
     2041 tccagggcca ctgctccacg tcggacatgt cgatcaacga cgcgttgatg gaacagctca
     2101 tcatggtcga cgccgcccgg cgtgcctcgg ccaagcgcat caccgtggtg gcgcccttct
     2161 acggctacgg ccgccaggac cgtaaggccg agggtcgcga gccaatcacc gccaaactcg
     2221 tcgcgaacat gttcaagacc gcgggcgcca agcgcctcgt ctcggtcgac ctgcactccg
     2281 gtcagatcca gggcttcttc gacggccccg tcgaccacct gaccgccatg cccgtgctgg
     2341 tcgagtggat ggcgaacaac ctccgcggtg agctcgttgt cgtgtcgccc gacgccggcc
     2401 gcgtgaaggt ggccgagcgg tacgccaatc tgctccacgc caacatcgcg atcgtgcaca
     2461 agcgtcgggt tcgcggcgtc aagaacgctg tcgaggccaa agatgtcgtc ggcgaggtcg
     2521 agggcaagac gtgtgtgctc atcgacgaca tgatcgacac cggtggcacg ctcgtcgctg
     2581 cggccgagca actcgccgag cgcggcgccg cgtcggtgta cgcggcggcc acccacgggg
     2641 tgttcagcgg ccctgccatc gatcgcatca agaactcggt cctcgagaag gtcgtgatca
     2701 ccaacacgct gccgctgccg cccgagaagc agatcgacaa gatcgaggtg ctgtccgctg
     2761 ccggcatcat cgccgatgcg atcgacgccg tgttcgagga cacgtccgtc tccgacatct
     2821 tcgacggaca gaaccaacac tgacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnngtgttc gaggacacgt ccgtctccga catcttcgac ggacagaacc aacactgacg
     3001 gaggccacct ctggtcgaat gccagccccg ttcctcggct gacctcgacc tccggcatcc
     3061 ttcggctggt cacggttggc tgctgcgagt atcctttccc gtcggccctt gcgcgtcccc
     3121 gcgccgccgc cacgttcgct tcgaggagtt gtttgctatg gctgaggtca ccctgaccgc
     3181 caccactggt cgcccgttgg gctcgagcgc ttcgcggcgt ctgcgcgccg agggcaagat
     3241 ccccgcgacc gtgtacggca tggacaagga cgcggtgtcg gtcacgatca ctcgctccga
     3301 cctccgcaag gcgatgacga ccgatgcggg cgtcaacgct ctcatcaagc tcgagatcga
     3361 cggcgccgcc accgagtacg ccctcgtcaa ggagatccag cgtcacacgg tccgtcgcga
     3421 ggtcacccac ctcgacctgc tccgcatcga tccggagacg gcgatggtgc tcgacgtgcc
     3481 gatcgtgctc accggtgacg ccaagaaggt gtccggcggc ggcggcttcg tcgaccagaa
     3541 gctgtcgacg ctgcgggtca cggttcgccc cgactccatc cccacgcagc tcacggccga
     3601 catctcggcc atggacgtcg aggacgtgct caccatcgcc gacctcacgc tgccgcgggg
     3661 agtcacgacc gagctcgatc ccgagctgcc gatcgtgacc gctgcgctca cccgtgccgc
     3721 catcgtggcc gcccgcgccg cagcgaaggc cgaagaggcc gctgctgcgg gtgcgtagcc
     3781 tcagcatccg attcgggtgg tgatgcgccg cggcgcacgt tccctcggcg ccgcgtcgag
     3841 cgccaagcag cacaacaaca caccgtggga cctcttggtc gttggactcg gcaatcccgg
     3901 gcccaagtac cgcggcaccc tccacaacgt cggggtcgag gtgctcgacc tgctggccga
     3961 gcggcatcgt gagaggttcc gcagctccaa ggagcatgcg ctcgtcgccg agctccgcat
     4021 cggagcggcc cgtgtcgccc tggcttttcc ccagacgtac atgaacgagt ccggccgctc
     4081 ggtcggccca ctcgtgaagc ggtacggcat cgacgacctc accaagctgg tggtcgtcca
     4141 cgacgagctc gatctcgatc cgggccggct caaggtgaag ttgggcggcg gtctcgccgg
     4201 gcacaacggc ctgaggtcga tcaaggcgca cctgcactcc gacgagttcg cccgtatccg
     4261 catcggcgtc ggcaagccgc ccggtggcaa ggaacgcggc gtcgatcatg tgctggcacc
     4321 accgtccaaa cgcgaccgtg agctcatcgc cgtgtgcgtc gaggaggccg ccgacgccgt
     4381 cgagatgatg ctgagcgacg gcatcgacgc cacgatggcg cgctacaacc agcgatgacc
     4441 gacgtggccg aatcccgagt cgcggccgtg gcaccgctgc gctcgctgcc cgggttgttg
     4501 cgcgaggaac cggcgctgct cggcaccctg ggtcggtcga gcgcgacgct cgccgtcccc
     4561 gagccggccc gggcggtgac gatcgccgcg atcgccacgc tctccaagcg atcgcccatc
     4621 gtcgtggccg tgccgacgag cgccgacgcc gaacgactcg ccggcgacct ggcctgtttc
     4681 ctcggggccg acgaggtcga cgtgttcccg gcctgggaga cgctgccctt cgaacgggtc
     4741 agtccgtcgg tcgagaccat gggacgccgc atgcgcacca tgtggcacct ccgcgacccc
     4801 gagcggtctc ccaaggtgct cgtcgctccc gcccgggcac tcgtgcagcg cctcgggccc
     4861 cacgtggagg acgtcgagcc gatcgtgatc acgccgggag cgatcgtcga ccaggccgat
     4921 ctcgtggagc gcctggtcga ggcggggtac cgccgcgagt accaggtgga gcaccgcggc
     4981 gagatcgcgg tgcgtggctc gatcgtcgac gtgttcccgt ccaccgccga cggccccgtc
     5041 cgaatcgacc tgtggggcga cgaggtcgag cggctcaccg acttctccgt caacgaccag
     5101 cggtccgaca acgacgtcga gcgcgtcgag atcttcccgt gccgcgagct gatgacgacc
     5161 gacgacgtcc gtgagcgcgc cgaggcgctg atcgagtcgg aaccctgggg tcgtgaccaa
     5221 tgggagaagc tggccgacgg ccagagtttc gacggcatgg agtcgtggct gccgtggctc
     5281 accggcgacg gcgccaccac ggcccacgtg ttgttcgacc tggtcggtcc ggagtcgcag
     5341 gtgatcctcg tcgacccacg ccgcatgcgg gaccgggcgg ccgacatcat cgccgaagag
     5401 gtcgacctcg cccgcacgct ctccgggacg tggggtgcga gcgacgacgg ggtgctcccg
     5461 aggctccacc tcgacttcga gcgcctgctg acccgcacga ccgctccgac gtggaccatg
     5521 acgaccgctc ccgagggccc cggcatcgaa accgtggccg cggcggcatg gttccaggcc
     5581 agcggcgacg ggtcccggct ggtcaaccag cttcgcgatc tgtcggcgtc gggctaccgc
     5641 atcgtcgttg cggccgaggg tgccggctcg gccgaacgcg tcgaccgact cctcaacgag
     5701 cagggtgtgt cctaccggct ccgtcccgag ggcggtgccg cgttggccga gcccgggggt
     5761 cacatcaccg tcgcggcgct caaccgcggt ttcatcctcc cgtcggtgaa gctggcggtg
     5821 ctgagcgaga gcgatctcac gggccgacgc cgcacccatc ggccggctcg gcagcgcaag
     5881 cgtcaggcgc agggtttctt cgacgacctc aaggtcggca gctacgtggt gcaccagcac
     5941 cacggcgtcg cccggttcgg cggcatggtc aagcgcacca tcggtggcgc ggaccgtgac
     6001 tacctgctgc tcgagtaccg cggcgacgac aagctgtacg tcccgtccga ccagatcgac
     6061 gccgtccgcc actacaccgg cggcgactca cccagcctct cgaagatggg tggcgccgac
     6121 ttcgcccgca ccaaggccaa ggtcgcgtcc gaggtggcga ccatcgccca ggagttggtg
     6181 gtgctgtacc agaagcgggt gcacgccaag ggccacgcct tcgccgagga ctcgccgtgg
     6241 cagaaggaga tggaggactc gttccccttc gccgagacgc ccgaccagtt gcaggcgatc
     6301 gaggacgtga aacacgacat ggaggcggag cgccccatgg accgcctggt gatcggcgac
     6361 gtcgggttcg gcaagaccga ggtcgcgatc cgcgctgcgt tcaaagcggt gcaggacggc
     6421 taccaggtcg cgatcctcgt gccgacgacc ctcttggccc aacagcacta cgcgacgttc
     6481 agtgaccggt tcgccccgta tccggtgcgt gtcgaggtca tgtcccggtt cctgaccgcc
     6541 aagcagagcc gcgtggtggc cgacggcctg aagtcgggcg aggtcgacgt ggtcgtcggt
     6601 acgcaccggc tgctgagcgg ggacgtggag ttcaagaagc tggggctgct cgtcatcgac
     6661 gaggagcagc ggttcggtgt ctcccacaag gaggccatca agcagttcaa ggccgacgtc
     6721 gacgtgctca cgctgacggc gacgccgatc ccacgaacca tggagatgtc gctcaccggc
     6781 atccgcgacc tgtcgctgct gcagacggca ccgtcggagc ggcagccgat cctcacctac
     6841 gtcggcgagt acgacgaccg ggccgtggcc gagtcgatcc gtcgcgagct cctgcgcgag
     6901 ggccaggtgt tcttcgtgca caaccggatc gccgacatcg aggagaaggc ggcagacctg
     6961 cgcgacctcg tgcccgaggc ccgcatcgcc gtcgcccacg gccagatgga cgagggctcg
     7021 ctggagcaga tcgtcgtcga cttctgggaa ggccgctacg acgtgctcgt ctgcaccacg
     7081 atcatcgagt cgggcatcga catgccgacg gtcaacaccc tcgtcgtcga acgcgccgac
     7141 ctcctcgggc tcgggcagct ccaccagttg cgcggccgcg tcggtcgagc cggaatgcgg
     7201 gcgtacgcct acctgttctt cccgcccgag cgggagctga gcgaggaggc ctacgagcgg
     7261 ctgcgcacga tcggcgaagc caccgagctg ggttccggct tccgcatcgc catgcgtgac
     7321 ctcgagatcc gcggcgccgg caacctgctg ggcaccggcc agaccgggca catcgcagcg
     7381 gtcggctacg acctctactg ctcgatggtc aacgaggcga tcaacgacct gaacggcgaa
     7441 accaaggccg aggacgtcga gatcaagatc gaactcccgc tcgacgcgaa ccttcccgcc
     7501 gactacgtgg agcgcgagga tctccgtctc gacgcctacc gacgactcgc cgaggtgaag
     7561 agtttcaccg acgtcgacga catcgccgcc gaatgggtcg accgctacgg cccggtgccc
     7621 gacgccgccg gtgcgctcct cgacatcgcc cgggtccgcg ccgagtgcgt ccgcaccggc
     7681 gtcaccgaca tctcggtgac gaagtcgacc gggttcggtg gtccgccgct ggtggcgcgg
     7741 atctcgccgc tgaagctccc gcagtcgaag cagatgcggc tggaacgcct gtacaagggc
     7801 gctgtctaca agaacgactc gcagctgcag ctgcccatca aggccgggcg agccatgatc
     7861 gccacgctgc tcgaggcgtt cacccagatg gtgcccgtcg acgagtgaac gacttgctcc
     7921 gccgatctgt gcccgtccaa ctccgcccag tacgatctga ggttctgtga agcgccttct
     7981 tgtcagctcc atcgccgccg ttgtcctcct caccgcttgt ggtgtcgccg aggacacgac
     8041 cgcggccacc gtcaacgggc aggacatctc ggtctcggcc gtgaccaagc tcgccaagtc
     8101 gaagtacatc gtggacacag cgtccgccgg ggggctcgtg tcctcgaagg atgtcgtggt
     8161 cggctcgacc acgcggcggg cggcgctcgg cctgttggtc cagtccgagg tctacgaggc
     8221 gggaatcgcc gctcgcaagg gcgagatcac cgatgtcgat cgcgacgctg ccgaggcgca
     8281 gatctccgac tacgagtcga acaaccagac gaccatcgac gacacggccc gtccggtcat
     8341 cgttcgactg atcgcggcgc aggcggccct cggccggatc ctgggtggcg cggacatcga
     8401 atcggcggcg tcgccggaga agatcgaggc gtacttcgac gagcacgccg acgagttcgc
     8461 caagatctgt gtcgatggct tcgcggtcac gagcgaccag gcggctgcgg cccaggcggc
     8521 ggtcgatcgt ggtgacgaca tcgctgcgat cctcgccgat tcgtcgttgc aggcgcagtc
     8581 cctctcgcaa accggcacgg agacctgtgt gctgtcgggc caggtgtcgg actccccact
     8641 cgccgacctc atcacgaccg gtccggtcgg ccagtggtcg agcaccgaca tctccgacgc
     8701 gacggcgggc gacctgaccg tcttcgttcg cccgaacagc cgcgacgagc cgacgctcga
     8761 cgatcccgac atctcggacc agatcgcgca gcagctccag gcggaggcaa cgtccgccgc
     8821 ccaggaagcg ttcggtgcgg aggcgcagac catcctcgag cgggccgacg tcgagatcga
     8881 ttcgcggtac ggccgctggg atccgtccga tccgtcactg atcctcgctc cgttggcgcc
     8941 cacgccgtcg gcgaaggcgg cggccgaggc cctgtcggcc gacgacctcg ctggtctcca
     9001 ggggcaatga tccccaccgt cgtcatcgtc gggctcggcc ccggcggcga cgacctcgtc
     9061 acgtcgggca cgctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctgagc
     9181 gacgtgaagc tctcgatcga ggagtggccg agcgagccgg tcacggtcat ccagcggttg
     9241 ggcctgcccg acgagcgcat cttcgaggtc gagtgggccg acctcgaccg tggtgtcgag
     9301 gcggaccacc tcacgtcgat ctacatcccg acgttggagc agccggttgc ggcggagttg
     9361 gtgcagttcg acgaactggt ccacgccctg cggaccgggt gcccctggga ccgggagcag
     9421 acccacgagt cgctcaagcg gtacctgctc gaggagtgct acgagctcct cgaggcgatc
     9481 gacgggttgg cggccgacac cgacgagagc atcgacgcgc tccaagaaga gctcggcgat
     9541 gtcctgttcc aggtgttctt ccacgcgacg atcgcggcgg agtccgggtg gttcaccctc
     9601 gccgatgtcg cccgcacggt gcacgacaag ttgcgcgacc gccatcccca cgtgttcggt
     9661 tccgccacct tcgagtcggc ggaggccctc gccgcgaact gggaggcgca gaagaagacc
     9721 gagaagtcac gcgactcggt catggacggc attccccccg aactgccggc gctgctctac
     9781 gcgctcaagg tgcagaagaa ggcggcggcg tcggactacc gcgagcccgt ggccgagttc
     9841 gtggcggcgc tcgatgcggg gacgatggac gccgcggcgc tgggggagtt gctgttcggc
     9901 gtcgtccagg tcgcccggac cgccggcctc gaccccgagg acgagttgcg acaggcctcg
     9961 cgccgcttcg tcgagcgctt cgaatcccgc tgacccggac cggtcagcga ctgacgccgg
    10021 cgcggcgatc gcgggcgagg cccaggagat agagctcgtc gtgggcgatc gtggcgccgg
    10081 ccagcgccgt gatctgaccg ctcgggtcgt agatcggggc gacctcgacg aggtcggcgc
    10141 cgacgatgtt caggccagcg aggccgaaca gcagttggcg ggcctgccag gtggtcggac
    10201 cgccgatgac cggcgtgccg gtgccggggg cgtaggccgg gtcgaggaag tcgatgtcga
    10261 acgtgaggta ggcgggccgg tcgccgacga tctcgcggat ctcggcgatc gtggcagcga
    10321 tgccgatgtc gaacaggcgg tcggcgtgga tcacgttgaa gccgtgcgac acgttgggtg
    10381 tgcgcatgcc cgtctgcacc gacgtcgccg ggtcgaggat gccgtcgcgg gcggctcgca
    10441 ggaacatcgt gccgtggttg agttcgtcgc cggcgtccca ggtgtcggag tgcgcgtcga
    10501 agtggatgag cgacaactgg ccgtggcgct cggcggtcgc gaggagcagg ggataggcca
    10561 cgaaatggtc cccgccgagg ccgaggaccg acgccccggc gtcgatgatg cggccaacat
    10621 ggtcgacgac gttggtcacc atctcctcgg gcaccccggg gcggtagccg atgtcgaagt
    10681 agtcgatgac cttgtggcgg tcgaagacgc tgtggtccca cggccagagg ccctcgggaa
    10741 actcggcgct gagcgtcgtc tgttcacgga tcgcccgtgg cccgaaccgt gcccccggtc
    10801 ggttcgtgac ggcgttgtcg taggggatgc cgacgacggc gatgtcgacg ccggtgaggt
    10861 cccgggtggc cggcagcctc ccgaacgaga gctggccggc ccaggtcatg tcgaagccct
    10921 gggcatcgcc gacgtcaccg gagaccgacg gatcgtggtt cgcttccatc gccgaatcct
    10981 cgcagatggc agatgcccga gcggagcgac cgacgtctgc ggggcccggc gatcgggcag
    11041 ggtgagagga ccgagacgag agggaccgag cagatgttgc aggtggagga cgtgcggtgg
    11101 cggtacggcg acaagatcgt gctcgacggg atcgacctgg aggtgccggc gggcaaggtg
    11161 atcggtctcg tcggaccgaa cggtgccggg aagaccacgc tcatgcggat cctgttcggc
    11221 gtcctcaccc ccgacacggg gagtgtgcgt ttcgacggcc gacccgctga cgaggaggct
    11281 cgccggtcgt gggggtacat gccgcaggag cgcggcctct accgtgacat gcgggtgctg
    11341 gatcaactcg tgtggattgc gcgattgcac ggcatcgacc gttcgacggc acgtcgccga
    11401 gcggtcgatc tgctcgaacg actggggctc gccgatcggc gcgacgacaa ggtcgaggca
    11461 ctctcgggag ggatggcgca gcgggtacag ctggctgccg cgatggtcca cgaacccgac
    11521 gtgctcgtcc tcgatgaacc gtttgccggg ctcgatccgg tggccgtgca gttcctctcc
    11581 gacgtgatca ccgatcacgc cgcggccggg cgtcacctcc tgttctccag ccaccaactc
    11641 gatctcgtcg aggagctctg cgagacggtg acgatgatgc accgtgggaa ggtcgtcctc
    11701 gggggtgatg tgcgccaact caaggccgac agtcccgatc ggacactgcg cgtggacgtc
    11761 gaggtcgaac actcgtgggt ggcgccgctc ggcgtcgagg tcaccgccgc cggcgctgcg
    11821 ggttcacgac tccgattggt gccgggggtg cgagccggtg aggtgctcga cgcggtgcgg
    11881 gcccaccacg aggtcaccga cttcggggtc gaggcgccga gcctcgccga gctgttcctc
    11941 gccgcagccg gcgacgtcac tgcgggggcg ctgcgatgac cgccgtcggg gccactcgat
    12001 tggtcgccgc tcgggagatc cgcgagacca cccgccgcaa gagcttctgg atcgtcctgg
    12061 tcgtcctcgc gctcgcagcg accgctgcgg tcgtggtgcc gaccctgctc gacgacgacg
    12121 gaacccgtta tcgcgtcgcg gtcgtcggcg atcgtcccgg actcgccgag gcgctcacga
    12181 cctcgctggc cgggctcgac gcgaccgttc gcacgtcgtt ggtcacgacg gccaccgatg
    12241 cggagcggag ggtggaggac ggttcggccg atctggcgat cgtcacgggt gatcgtgccc
    12301 ggctgatcgt gaaggccggt gagcaccagg aactggtggg tgccgcgagt caggcgttgt
    12361 cgaccatcgg gttcgccgcg gatctcgagg ccgagggcgt ggacgccggt cggatcaccg
    12421 acctgttgag ccgccccgcc cccacggtca cagagatcga ccggggcgcc gccgatcgca
    12481 agggcgcttc cttcgcgatc acgctcgtgc tctacatcct cctcctgacc ctgatggtgc
    12541 aggcggcgaa cggcacggcg atcgagaagt cgaatcggat cagcgaggtc ctcttggcga
    12601 tcgtccgtcc cggcccgctc ctgttcggca aggtcatcgg catcagcgtc accgggctgc
    12661 tgaccttcgc cgccggcggc atccccatcg tcatctccct ggcgatcggc agcgatctcc
    12721 cgcccgggat cggcggtgcg ctcgccggtg gcgcgatctg gttcgtgctc gggatggtgc
    12781 tgtacctgac cctcgccggc tccctcgggt cgttggccga gcgccaggag gaagccggcg
    12841 tcgtcgtggc accgctcacc ttcctgctgg tgggcacgtt catcgccgca cagagtgcgg
    12901 ccgacacgac gttcgggatc gtcctcgccc tggtaccgct cacgtcgccg ctggtcatgc
    12961 cggcccggat cgccgaagga gtcgcgaccg ttcccgagat ggcggcgtcc gcgctgctcc
    13021 tggtcgcagc gatcgcgctc gtggcccgct tcggtgcggt ggtctacggc agggccatcg
    13081 tgcggaccgg ccggcggctc aagctcaagg acgtgctgcg gtccaccccg gcctgagtgg
    13141 gcagattttg tcgccggggg gtcactgtgg tgcgatgtcc tccgcactat cgtcgtcgga
    13201 accatcagag ggcagtcatg agcatcatcg aacacattca cgggcggcag atcatcgatt
    13261 cgcgcggcaa cccgaccgtc gaggtcgagg tcgtgctcac caccggcgcc gccggtcgtg
    13321 ccgccgtgcc ctcgggtgcg tccaccggtc agttcgaggc cgccgagctg cgtgacggcg
    13381 ggtccgacta cctgggcaag gccgtcacga acgccgtcga ccacgtcaac gacgagatcg
    13441 ccgagcacat cgtcggcctc gacgccctcg accagcgcca gatcgaccga atcctcgtcg
    13501 acctcgacgg cacacccaac aagagccggc tcggcgccaa cgccatcctc ggtgtgtcgc
    13561 tcgctctcgc ccgtgctgcc gccgaagagg tcggcctccc gctcttccgt tacgtcggtg
    13621 gcgccaacgc ccacgtgttg ccggtgccga tgatgaacgt cctcaacggc ggtgagcacg
    13681 ccgacaacaa cgtcgacgtg caggagttca tgatcatgcc ggtcggcgcc gcctcgttct
    13741 ccgaggcgat gcgctggggc atcgagacct accacgtgct caagaaggtc ctccacgaca
    13801 agggcctctc gaccgccatc ggtgacgagg gtggtttcgc ccccaacctc ggctccaacg
    13861 aggaagcggt gcagctcctc tgttcggcca tcgagcaggc cggctacacg cccggtgagc
    13921 agatcgcgat cgcgctcgac gccgccagct ccgagttcta caaggacggg aagtacgtgc
    13981 tggccggcga gggtcgttcg ctcgattcgg ccgagttcgc gggctacctc ggcgagctgt
    14041 gctccaagta cccgatcgtg tcgatcgagg acggcatgga cgaggaggac tgggagggct
    14101 ggtcgctcct gaccaagcag atcggcggct cggtgcagct cgtcggcgac gacttgttcg
    14161 tgaccaacgt cgcccgtctg tcgcgtggca tcgaggttgg cgtcgccaac tcgatcctcg
    14221 tcaaggtcaa ccagatcggc acgctcaccg agacgctcca gtccgtcgag ctggcgcacc
    14281 gcaacgccta cacggctgtg atgtcgcacc ggtccggcga gaccgaggac acgacgatcg
    14341 ccgatctcgc ggtcgccacc aactgcggcc agatcaagac cggtgccccg gcgcgttcgg
    14401 accgtgtggc caagtacaac cagctgctcc gcatcgagga gc
//
LOCUS       DAOSOA010000041        14298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4270_length_14298_cov_5.977673, whole genome shotgun sequence.
ACCESSION   DAOSOA010000041 DAOSOA010000000
VERSION     DAOSOA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4270_length_14298_cov_5.977673"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PKY13_04480"
     CDS             <1..982
                     /locus_tag="PKY13_04480"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOT79215.1"
                     /translation="DTEIPVSISVGIATSDLDGGPGALLRDADTAMYGAKDAGRDSVL
                     VFDNSMRDRVSNRLELERDLRHALDRDELAVHFQPVVDMVNSRTLGFEALMRWNHPMR
                     GTVPPLEFIPIAEETGVIVEIGAWILDHACQQLAYWRGTAPEYANLWCSVNVSARQLR
                     DADFVFMVERSLAKHALPPDALVLEVTESLLVDEGPMVPAILNRIRDHSIRLSIDDFG
                     TGYSSLAYVQRFPFSCVKIDRAFVDPLDNEGGGEHQLVGAIVAMAAALGLDTIAEGVE
                     TQAQADRLVELGCVEAQGYLYSRPVPPEQIPATLRRLNQAWLRPLASATQ"
     gene            1154..1546
                     /locus_tag="PKY13_04485"
     CDS             1154..1546
                     /locus_tag="PKY13_04485"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HOT79216.1"
                     /translation="MAIHELRREGIVVPETQRVDLAPDIHRQRLVIEGNVTQRISAAK
                     IKRYLTGLSHVTDMVPLMTPMTHRSDLYGWAGWIHWETSGAHFYAWDQPRRFFSVDMY
                     TCKAFDPQDAVDYTVDFFEPTETTWFGF"
     gene            1550..2539
                     /locus_tag="PKY13_04490"
     CDS             1550..2539
                     /locus_tag="PKY13_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79217.1"
                     /translation="MEPRLGSGSADDTSTEEPHPDLVIDLTEGSTRPGVLRPGNVDGL
                     DLWSEDYRTPEYLAWAIDDLRREFGEPDQRYGVYFIHGEDPRSGLSRAVELERFGDSF
                     NNDVPLMRRLYGQFEDVGATEIITVVDHEEHLPCGVIRTVRNTAEYGCRILNDLQKHG
                     EDGWGLTWDEIVARSRFAATRADEITDIPTVAVAIKYQGHRKADGVSKALYAGLMQQG
                     LRSDAKTWVCSLDRIPYILVQAAAKDIMQEFDGVPGQPYYGAPDTVPLWANIWDYEYY
                     LRTQHPDAYAMYAHSVGLDDQYFFGFAEPPTWVAPDPEVIDLRMYDPVVPESR"
     gene            complement(2437..3660)
                     /locus_tag="PKY13_04495"
     CDS             complement(2437..3660)
                     /locus_tag="PKY13_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT79218.1"
                     /translation="MTPPPPEEGGRAVPPRNKAPAESAASLSEGDRANQYEWRPLDGF
                     RSIRTKLSVLVAGSVTISVLIGSVGLRVGWPFWFRLITSVIVGLGIMRLVSKGITSPL
                     RDMASAARAMADGDYSRRVRDTSRDEVGELATAFNEMAAELAEVDRQRRELVANASHE
                     LRTPIAALQAMIENLADGVSEADPAFVDTMHNQVRRLAVLVDQLMSLSRLDSGEMPLH
                     IETVSVAALVQASLEEFSWHHPDAEIEVDVEPETLLVAVDQLLMHRVIANLLENAIRH
                     GAPPFHIAASLGPAQPGDRQQSGPTVSIVVSDGGPGIPAEAASQVFDRFHRLDRSRRS
                     GGSGLGLAIVAGIVERHHGVISVEPNTPTGVRMVVRLPQDYRDSGTTGSYMRRSMTSG
                     SGATQVGGSAKPKKY"
     gene            complement(3660..4349)
                     /locus_tag="PKY13_04500"
     CDS             complement(3660..4349)
                     /locus_tag="PKY13_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT79219.1"
                     /translation="MDQHGAQQRIVVIEDEEAIADAVMQRLRGEGFDVEWAADGIVGV
                     ELCERVRPDLVVLDLMLPGLDGLEVCRRIQAERRTPVLMLTARDDESDLLVGLAVGAD
                     DYLTKPFSPRELVARVRAVLRRAEPQPAPADEAPLVVLDIEIDRATRRVARASEPVHL
                     TPIEFDLLVCLALRRGAVLSREVLLAEVWGYDGDYGARTVDSHVRSVRRKLGDEVIRT
                     VHAVGYAIGSA"
     gene            4469..5143
                     /locus_tag="PKY13_04505"
     CDS             4469..5143
                     /locus_tag="PKY13_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79220.1"
                     /translation="MPLCQRIRPVSIAAIALVSGVLLVGGCGGDDTADGADPTTTAAA
                     KPTSTTSAPTTTSSTTTTVADGGAGSTTTAAVGVTTTAGGSDAASADAVPDGTSFGYV
                     TAINIGGSTVTIDIAELLTGDAAVTAAIEDGALEPGETSIDNDYYVRNKNPKLRTVTV
                     GPTAPVNVLSSPGSPDLESGNLQKLAEALTAVPVGDPAEGGPRLPVQIVARGGLISRI
                     DEVFFP"
     gene            complement(5306..6250)
                     /locus_tag="PKY13_04510"
     CDS             complement(5306..6250)
                     /locus_tag="PKY13_04510"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: protein motif:HMM:NF005479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HOT79221.1"
                     /translation="MAEADTTPPAANALAESQLALRDRLVDAGRLIPTGVAGLFGRDV
                     VFEAVVSGVGALVAAAGEDDHPTRVSYPPLIPKVDFDKIGYLRNFPELVGPVFSFVGD
                     AADHRAVVERLDGDAPYGDLLTQTEVTLTPACCYPIYPSVTGALGAGGAVFETCAYCF
                     RHEPSVDPMRLQAFRQHEHIRIATPDEVLDWRARWLERAPELLSALGLDVISDIANDP
                     FFGRAGRLMKMSQREQQLKIEFLVDVYGDEHRTACSSINYHQDHFGHTFGIHTDDGAD
                     AHSSCIGFGLERCTVALFNQHGTDVAQWPAPVRDRLGL"
     gene            complement(6273..6527)
                     /locus_tag="PKY13_04515"
     CDS             complement(6273..6527)
                     /locus_tag="PKY13_04515"
                     /inference="COORDINATES: protein motif:HMM:NF005480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT79222.1"
                     /translation="MPDHTQLIRSVLVDHGRLAVDVATLSDSASLYEAGLTSHASVNV
                     MLALEDEFDVEFPERFLKRTTFESIENLSVALADLVHVDA"
     gene            complement(6527..7138)
                     /locus_tag="PKY13_04520"
     CDS             complement(6527..7138)
                     /locus_tag="PKY13_04520"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOT79223.1"
                     /translation="MPTAEIPAGMPAAVDWDGTKRRVAPRRGTDRPWALSTLRVGTDM
                     MAVDEVARSIERFGERYLRRVFSELERATCTGARGPAADRFAARFAAKESVIKALRPD
                     RPIAFHDIEVQLDPDGGPEIELRGNAANCAEALRVIDTSLSLTHDAGRASAVFVAVLD
                     TPLPSIALPAPTLDPASKLSDLFPAGIPFDRRYDTYDHSQEAV"
     gene            7467..7625
                     /locus_tag="PKY13_04525"
     CDS             7467..7625
                     /locus_tag="PKY13_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79224.1"
                     /translation="MITRNELDDVNDRLFEVAAVVRDAQLQLEAGLSPIKVLEHLLVA
                     VDSVVGTN"
     gene            complement(7736..8080)
                     /locus_tag="PKY13_04530"
     CDS             complement(7736..8080)
                     /locus_tag="PKY13_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT79225.1"
                     /translation="MSVAVDDDLWSAIGDPTRRRMLDLLLDHGPGTATSLSAHLPVTR
                     QAVAKHLGVLDRVGLVNVTPIGREMRYDVDEAQLDRAIAQLTTVGSTWDARLRRIKRL
                     AESIQASVDGVE"
     gene            complement(8077..8568)
                     /locus_tag="PKY13_04535"
     CDS             complement(8077..8568)
                     /locus_tag="PKY13_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT79226.1"
                     /translation="MTTGSTQDQSTEFGSIERELYIEASPEVVYAVISRPEHIAEWWA
                     DEVDLDPTPGAVGELAWNNGPTKRAAVEPITVVEADPHRRFSFRWIAEGEAAAPGNSL
                     LATFDLEPRGSGTLLKVSETGFREKGWDAAVLEQAYRDHCNGWDLHLPRIVPVAEQIA
                     SGS"
     gene            8770..9285
                     /locus_tag="PKY13_04540"
     CDS             8770..9285
                     /locus_tag="PKY13_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOT79227.1"
                     /translation="MTLTIRLLVALGLTWAIGFERELRGAAAGDRTFALIGVGSAVIG
                     YLALDHAPNALAGVVTGIGFIGAGLIVHGALPNRPGAPDGADVDAAVDVAAGSGAIGR
                     IRGVTTAATIYLAAAVGAAAGQGSLLLAFEATIGCLLLLEVRHLRWLRFLDGRFWRHR
                     LHLPDDDPADL"
     gene            complement(9310..11727)
                     /locus_tag="PKY13_04545"
     CDS             complement(9310..11727)
                     /locus_tag="PKY13_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT79228.1"
                     /translation="MAPTPSPLHRIAAVLKDHSRWLSSKVALGVTVALFVSAGLLAGM
                     ASLQRSAADDRVAEQGWVRLSKARLDILGAIGELDTALASSGSRDDAVAAFVARRAAI
                     DEDLADLAKVSPDLAEPVRSLHDSLTAVPEDTTDVERLHIGITQIAGEVANGSNLSPL
                     AGDLAFADRLAADVAHLQLADRLIGIETERADLGGDHSYALSIAADLARNAEDHLWRF
                     GTRAISADVPESVRADLTSATTSTEARLVQDAATWAAARAGLDVDAGSVSTPDATAVD
                     AAAGTLSRDLIAMGSKVITGQARQLGLDEANGRDLSVKLFYGIVLCIAAATVILALRR
                     KRSRISDDELRAAASTDRLTGLGNRVHLDEEVALLAERSPEVVLMHIDLDHFKPVNDT
                     YGHAVGDRVLQLSAQRLREVADTHDGIAARLGGDEFVLVLPSIEQKIVDDVTHSLLQS
                     LQSFQIGGLDLTVGASIGVARGVGGVTDLLINADLALYQAKRTGRGQASTFRAEAAAF
                     VSFVRDALMDGKVQVVYQPQVSLRDGRCIGAEVLARLVDSTGSLIPAKDWLGIAEWLG
                     VTGELFEHVVSAVCDELASGPPVHTKLWFNVAPNDLIRPGGTDWVLSQLSRLKLPVSM
                     IGIELTETEAITDPASLAVVLERLKSVGLGLALDDFGARNTPIGHLVDLPVTRVKLDA
                     SLIAGIGEDMPPSSWVVKAMAELATRLRIEVMAEGVTSGHQVSLLAKLGVPAAQGYLL
                     GLPGPLDRLPTAVNIRSLLQTSLNGFDAARPVGPLVQMSGNRTGSATERAHVARSEVG
                     DRRGGGR"
     gene            11799..12569
                     /locus_tag="PKY13_04550"
     CDS             11799..12569
                     /locus_tag="PKY13_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HOT79229.1"
                     /translation="MPKFSGRSDRAADRSTDGAVDRSPSRQGGALLSTVDRIVESRYD
                     SAVALVDGIRARPGRPSTDEIIDELVRKYSKELAAVAALSGGAAALPGTGTAAAVLAT
                     GADVAFTIGKLAEMVLAIGVAHGHDARSIEERRAWVLAVLSMGKGAVSGVEGLAGRIG
                     AEGGARIVTTITSTQLDSVNSRLAAKMLARLASEQSAARLGRLLPFGIGAGVGAAGNV
                     LIVRSVARTARKFFAVGPSTAPILEVDPDVIDVEVLED"
     gene            12591..14018
                     /locus_tag="PKY13_04555"
     CDS             12591..14018
                     /locus_tag="PKY13_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT79230.1"
                     /translation="MATPVLTPALIDGTDVTDGEVTEVRSPFDGRVVGLVPSLTTDHV
                     DRAVAVALERHRGPQLPAHQRAEILERAAVLLTERNEEFGQSISAESAKPITTARIEA
                     SRAVDTLRFSAVQARTLVGEMVAMDATPAGVGKLGYVKRVPIGVIGAISPFNFPLNLV
                     CHKIAPALAAGCPVVLKPASATPLTALKIARLFEEAGLPPGWLNVVTCPGSVADRLVT
                     HDDVAMITFTGSPEVGWGIRARGARKRVSLELGNNAPVVVEADADLELAATKIVAGGF
                     AFSGQTCISVQRVYVHSSVHDELLDLVTAKVEQLVVGDPADDATVVSALIASKETKRV
                     KDWIDEAVAAGARLVVGGDLTPDEVLKPTVLDNVTADMKVCSDEVFGPMIGVASYDDY
                     EDALARANDTRYGLQAAVFTKDLAKALRAADVLDFGGVLINEMPSWRADQQPYGGVRD
                     SGNTREGPAYTVQEMTERRVIIITG"
BASE COUNT         2223 a         4870 c         5008 g         2197 t
ORIGIN      
        1 tgacaccgag atcccggtgt cgatcagcgt cggtatcgcc acgtccgacc tcgacggcgg
       61 gcccggggcg ctgctccgcg atgccgacac tgcgatgtac ggggcgaagg atgccggccg
      121 ggattcggtc ctcgtgttcg acaactcgat gcgcgaccgg gtctcgaacc gactcgagct
      181 cgagcgggat cttcgtcacg ccctcgaccg cgacgagctc gccgttcact tccagccggt
      241 cgtggacatg gtcaacagcc ggacgctcgg gttcgaggcg ctcatgcgct ggaaccaccc
      301 gatgcgtggc acggtgccac cgctcgagtt catcccgatc gccgaggaga ccggggtcat
      361 cgtcgagatc ggcgcgtgga tcctcgatca cgcgtgccag cagctggcgt actggcgggg
      421 cacggcgccc gagtacgcca acctgtggtg ttcggtgaac gtgtcggccc gacagcttcg
      481 cgatgccgac ttcgtgttca tggtcgagcg ctcgctggcg aaacatgcgc tgccgcctga
      541 tgccctcgtg ctcgaggtca ccgaatccct cctcgtcgac gagggcccga tggtcccggc
      601 gatcctgaac cgcatccgtg accacagcat ccgcctgtcg atcgacgact tcggcaccgg
      661 ctattcgtcc ctcgcctacg tccagcggtt cccgttcagc tgcgtcaaga tcgaccgtgc
      721 cttcgtcgat ccgctcgaca acgagggcgg cggtgaacac cagctggtcg gggcgatcgt
      781 ggcgatggcc gccgcgctcg gactcgacac catcgccgaa ggggtcgaga cccaggcgca
      841 ggcggaccgt ctcgtcgagc tcggatgtgt ggaggcgcag gggtacctgt actcccgccc
      901 ggtgccgccg gagcagatcc cggcgacgct gcgccgcctg aatcaggcgt ggctccgccc
      961 cctcgcgtcg gcgacgcagt agcggtcggc cggcggacac gcccgcatcg cgagaacatc
     1021 gttcttgcga tgcggtgacg cgcgatcgag tgacctaggt catcctgcct agcgtccccc
     1081 ggccaaagca cctaggattt cccggccagc acggtcacct gggagagggg cgccgtggtc
     1141 caaggaggga tctatggcca tccacgaatt gcgccgcgag ggaatcgtcg ttcccgagac
     1201 ccagcgcgtc gatctcgccc ccgacatcca tcgccaacga ctggtgatcg agggcaacgt
     1261 cacccaacgc atcagcgctg cgaagatcaa gcgatacctg acgggcctgt cccacgtcac
     1321 cgacatggtt ccgctcatga cccccatgac gcaccggtcg gacctctacg gctgggccgg
     1381 ctggatccac tgggagacgt ccggagcgca cttctacgcc tgggaccagc cgcggcggtt
     1441 cttcagcgtc gacatgtaca cgtgcaaggc gttcgatccc caggacgccg tcgactacac
     1501 cgtcgacttc ttcgagccca ccgaaacgac ctggttcggg ttctgagtca tggagccgcg
     1561 actcggttcc ggatctgccg acgacacctc gaccgaggag ccacaccccg acctcgtcat
     1621 cgacctgacc gagggctcga cgcgcccggg ggtgctccgc cccggcaacg tcgacggcct
     1681 cgacctgtgg tccgaggact accgcacccc cgagtacctg gcttgggcga tcgatgatct
     1741 ccggcgcgag ttcggcgaac cagatcagcg ctacggcgtg tacttcatcc acggtgagga
     1801 cccgcggtcg ggcctctcgc gcgcggtcga gctcgagcgc ttcggcgact cgttcaacaa
     1861 cgacgtgccg ctcatgcgcc gtttgtacgg gcagttcgag gacgtcggcg ccaccgagat
     1921 catcaccgtc gtcgaccacg aggaacacct cccgtgcggc gtcatccgca cggtccgcaa
     1981 caccgccgag tacggctgcc gcatcctcaa cgacctccag aagcacggcg aggacggctg
     2041 gggtctgacg tgggacgaga tcgtggcccg gtcccggttc gccgccaccc gcgccgacga
     2101 gatcaccgac atcccgaccg ttgccgtcgc catcaagtac cagggccacc gaaaggccga
     2161 cggggtgagc aaagcgttgt acgccggcct gatgcagcag gggctgcgca gcgacgccaa
     2221 gacctgggtg tgctcactcg accggatccc gtacatcctc gtgcaggccg cggcgaagga
     2281 catcatgcag gagttcgacg gggtaccggg ccagccgtac tacggcgcac ccgacaccgt
     2341 gccgctgtgg gccaacatct gggactacga gtactacctg cgcacgcagc accccgacgc
     2401 gtacgcgatg tacgcccact cggtggggct cgacgatcag tacttcttcg gtttcgccga
     2461 gccgccgacc tgggtcgcgc ccgaccccga ggtcatcgac ctgcgcatgt acgacccggt
     2521 ggtgcccgag tcgcggtagt cctggggcag ccgcacgacc atgcgtaccc ccgtcggcgt
     2581 gttcggctcc acgctgatca ccccgtggtg gcgctcgacg atgccggcga cgatcgcgag
     2641 cccgagaccg ctgccgcccg aacggcggga gcggtcgagg cggtggaacc gatcgaagac
     2701 ctgcgacgcc gcctcggcgg ggatgcccgg accaccgtcg ctgacgacga tcgacaccgt
     2761 ggggcccgac tgctgacgat cacccggttg cgccggcccg agcgacgcgg cgatgtggaa
     2821 cggcggagca ccgtggcgga tcgcgttctc caacaagttg gcgatcaccc ggtgcatcag
     2881 cagctggtcg acggccacga ggagggtctc gggctcgacg tcgacctcga tctcggcgtc
     2941 ggggtggtgc cagctgaact cctcgagcga cgcctgcacg agtgccgcca ccgacacggt
     3001 ctcgatgtgg agcggcattt cgccggagtc caggcgggag aggctcatga gctggtcgac
     3061 gagcacggcg aggcgccgca cctggttgtg catggtgtcg acgaaggcgg ggtccgcctc
     3121 gctcaccccg tcggcgaggt tctcgatcat cgcctggagg gcggcgatgg gggtacggag
     3181 ctcgtgggac gcattcgcca cgagctcacg gcgttggcgg tccacctcgg ccagctcggc
     3241 cgccatctcg ttgaacgcgg tggcgagctc gcccacctcg tcacgtgacg tgtcgcggac
     3301 gcggcggctg tagtcgccgt ccgccatggc ccgcgccgcc gacgccatgt cgcggagcgg
     3361 cgacgtgatg cccttcgaca cgaggcgcat gatgcccagg ccgacgatca ccgacgtgat
     3421 gagccggaac cagaacggcc agccgacgcg gaggccgacg ctgccgatga gcaccgagat
     3481 cgtcaccgag ccggcgacga gcaccgacaa cttggtgcga atcgagcgga acccgtcgag
     3541 cggccgccat tcgtactgat tggctcggtc accttcgctc aggctcgctg ccgactccgc
     3601 cggcgccttg ttgcgcggcg gtactgctcg accgccctcc tccggtggcg ggggagtcat
     3661 caggcgcttc cgatcgcgta ccccacggcg tggacggtgc ggatcacctc gtcgccgagt
     3721 ttccgtcgga ccgatcgcac gtgggagtct acggtgcggg cgccgtagtc gccgtcgtag
     3781 ccccagacct cggcgaggag cacctcgcgg ctcagcactg cgccgcgacg cagggcgagg
     3841 cacacgagga ggtcgaactc gatgggggtc aggtgcacgg gctcgctggc acgagcgacg
     3901 cgacgggtgg cacggtcgat ctcgatgtcg agcaccacga gcggtgcctc gtccgcgggc
     3961 gccggctggg gctcggctcg gcgcaggacc gcccggacac gggcgacgag ttcgcggggg
     4021 ctgaacggct tcgtgaggta gtcgtcggcc ccgacggcga gaccgacgag gaggtcggat
     4081 tcgtcgtcgc gggcggtcaa catgagcaca ggcgtccgcc gctcggcctg gatgcggcgg
     4141 cacacctcga ggccgtcgag tccgggcaac atgaggtcga gcaccacgag gtcgggccga
     4201 acgcgctcgc agagttcgac cccgacgatg ccgtcggccg cccactcgac gtcgaacccc
     4261 tccccacgga gacgctgcat caccgcgtcg gcgatggcct cctcgtcctc gatgacgacg
     4321 atccgctgct gtgccccgtg ctgatccacc cctccacgtt agagatcacg ggcgtcggag
     4381 ttgtgcgaac gatgtgcagg cccggtgaag aaccgaggtg cgaccgtgcg caccggaacc
     4441 cgtcggcgac cgaactacga tgccgcccgt gccactctgc cagcgcatcc gtccggtgtc
     4501 gatcgccgcg atcgcgctgg tttcgggtgt gctcctggtt ggcgggtgcg gcggcgacga
     4561 caccgccgac ggtgccgatc ccaccacgac ggcagcggcg aagccgacga gcacgacgtc
     4621 cgcgcccacc acgacctcgt cgaccaccac cacggtcgcc gacgggggag cggggtcgac
     4681 caccaccgcc gcggtcggtg tcacgacgac cgccggcggc agtgatgccg cgtccgccga
     4741 cgctgtgccc gacggcacca gcttcgggta cgtgaccgcg atcaacatcg gcgggtcgac
     4801 cgtcacgatc gacatcgccg aactgctgac cggcgatgcc gcggtgacgg cggccatcga
     4861 ggacggggcg ctcgaaccgg gcgagacgtc gatcgacaac gactactacg tccgcaacaa
     4921 gaacccgaag cttcggacgg tcacggtcgg gccgacggcg ccggtgaacg tgctctcgag
     4981 cccgggcagc cccgacctcg agagcgggaa cctccagaag ctggccgagg ccctcaccgc
     5041 agtgcccgtc ggcgatccgg ccgagggcgg cccgaggctg ccggtccaga tcgtcgcccg
     5101 tggtgggctc atcagtcgta tcgacgaggt gttcttcccg tagcgcccgg gtgggcggcg
     5161 ctcgaagtgg tgcgctggcg cgacgtttcc atcgcgctca cgcaccactt cgatcgacca
     5221 cgatcgagcg atgccgacga tcacgcgctc acgtcggagt tcgagctggc gacccgccgc
     5281 ctcgaacccc ttggaactcc tcggcttaga ggccgagccg gtcgcgcacg ggcgccggcc
     5341 actgcgcgac atcggtgccg tgctggttga agagcgccac ggtgcaccgc tcgagcccga
     5401 acccgatgca cgacgagtgc gcgtcggcgc cgtcgtcggt gtggatcccg aacgtgtggc
     5461 cgaagtggtc ctggtggtag ttgatcgacg agcacgccgt ccggtgttcg tcgccgtaca
     5521 cgtcgacgag gaactcgatc ttgagctgct gctcgcgctg gctcatcttc atgaggcgcc
     5581 cggcgcggcc gaagaacggg tcgttggcga tgtcggagat gacgtcgagg ccgagggccg
     5641 agagcagctc gggcgcccgc tcgagccagc gcgctcgcca gtcgagaacc tcgtcgggcg
     5701 ttgcgatacg gatgtgctca tgctggcgga atgcctgcag gcgcatcgga tcgaccgacg
     5761 gctcgtggcg gaagcagtag gcgcacgtct cgaacacggc accaccggcg ccgagcgcgc
     5821 cggtcaccga cgggtagatc ggatagcagc acgccggggt gagcgtcacc tcggtctgcg
     5881 tgaggaggtc gccgtagggg gcgtcgccat cgagccgttc gacgacagcg cgatgatcgg
     5941 cggcatcgcc gacgaacgag aagaccggcc cgacgagttc gggaaagttg cgcaggtagc
     6001 cgatcttgtc gaagtcgacc ttcgggatca gcggcgggta cgacacccgc gtcgggtgat
     6061 cgtcttcgcc agcagcggcg acgagcgcgc cgacgcccga aacgacggct tcgaacacga
     6121 catcgcggcc gaagagccct gcgacgccgg tggggatgag gcggccggcg tcgaccagcc
     6181 ggtcccggag tgccagctgc gactcggcga gtgcgttcgc ggccggggga gtggtatcgg
     6241 cctcggccat ggcgggagtg gtacggcgcc ggtcaggcgt cgacgtggac gaggtccgcc
     6301 agcgcgacgc tgaggttctc gatcgactcg aaggtcgtgc gcttcaggaa acgctccggg
     6361 aactcgacgt cgaactcgtc ctcgagggcc agcatcacgt tgacgctggc gtgcgacgtg
     6421 agaccggcct cgtacaggct ggccgagtcg ctgagggtgg cgacgtcgac cgcgaggcgg
     6481 ccgtggtcga cgagcacact gcggatgagt tgggtatggt caggcatcag acggcttcct
     6541 gggagtggtc gtaggtgtcg tatcggcggt cgaaggggat gcctgcagga aaaaggtccg
     6601 acagcttgga cgcggggtcc aacgtcggcg ccggcagggc gatcgagggc aggggagtgt
     6661 ccagcaccgc gacgaacacg gccgacgctc ggccggcgtc gtgggtgagg ctgagcgagg
     6721 tgtcgatcac acggagggcc tcggcgcagt ttgcggcgtt gccccggagc tcgatctcgg
     6781 gcccaccgtc ggggtcgagc tggacctcga tgtcgtggaa cgcgatcggc cggtcaggtc
     6841 gcagtgcctt gatcaccgac tccttcgctg cgaaccgcgc tgcgaagcga tccgccgcgg
     6901 ggccgcgtgc gccggtgcag gtcgcccgtt ccagctcgct gaacacgcgc cgcaggtacc
     6961 gctcgccgaa ccgctcgatc gagcgggcga cctcgtcgac ggccatcatg tcggtgccga
     7021 cgcggagtgt gctcaatgcc cacggacggt cggtcccccg gcggggcgcg acccgacgtt
     7081 tggttccgtc ccagtcgacg gcggccggca tcccggcggg aatctccgcg gtaggcatgc
     7141 ctccttcatc ggtccgctcc gccacaactt gaagattgaa ctagtcaatt tcccccaaat
     7201 cggttcggtc ttgatcagct gaactgaact gcccggagtc gggtggtcct tgacgggcac
     7261 aggcgcaccc cctaagtttc gtcacagtga cgaaacgcaa ggggtcaggg aagcggtcga
     7321 ggtgcgagtg gtgctcgcgc ccgttgccgg aacggccggc cgaccgggtg gggcggaagc
     7381 cgaagtactg ccgtcggagt tgtcgccaac gagcgttcga ggcacgtcgg cgtggtgaac
     7441 agctgggcgt cgccgacgac gaactcgtga tcacgagaaa cgagttggac gatgtgaacg
     7501 accgactgtt cgaggtggct gcggtggttc gcgacgccca gttgcagctg gaggccgggt
     7561 tgtcaccgat caaggtcctc gaacatctcc tcgtggcggt cgacagcgtc gtggggacga
     7621 actgacgcac cccgctcggg tgctccgggg ctctctgctc aacgccggaa atacacgtta
     7681 tgtcaagttg aggtcgaggc ggtgggtggg ccgaacggcc tacccacccc cctgctcact
     7741 ccacgccgtc gaccgaggcc tggatcgact cggccaggcg cttgatgcgc cgaagccggg
     7801 catcccaggt ggatccaacc gtcgtcagct gggcgatcgc tcggtcgagc tgcgcctcgt
     7861 cgacgtcgta acgcatctct cgcccgatcg gcgtcacgtt gacgagcccg acgcggtcga
     7921 gcacgccgag atgtttggcg acggcttggc gggtgacggg caggtgggcg ctgagactcg
     7981 tcgccgtacc cggcccgtgg tcgaggagca ggtcgagcat ccgtcgccgg gtggggtcac
     8041 cgatcgccga ccagagatcg tcgtcgacgg cgacgctcac gagcccgacg cgatctgctc
     8101 ggcgaccggc acgatccggg gcaggtggag gtcccagccg ttgcagtggt cgcggtacgc
     8161 ctgctcgagc actgccgcgt cccacccctt ctctcggaag ccggtctcgc tcaccttgag
     8221 gagggtcccg ctcccccgcg gttcgaggtc gaaggtcgcc agcagcgagt tccccggagc
     8281 ggcggcctcg ccctcggcga tccaccggaa cgagaagcga cggtgcgggt cggcctcgac
     8341 gacggtgatc ggctcgacgg ccgcccgctt ggtggggccg ttgttccagg cgagctcccc
     8401 caccgccccc ggggtcgggt cgaggtcgac ctcgtcggcc caccactccg cgatgtgctc
     8461 gggccggctg atcacggcgt acaccacctc aggtgacgcc tcgatgtaga gctcgcgctc
     8521 gatgctcccg aactcggtgc tctggtcctg cgtgcttccg gtggtcatgg tcgtctcgct
     8581 ttctgcaacg tttggttgcg gaagaaccta cgcgcctcgg ccgtgatgtg caaccagaag
     8641 ttgcggtttg gttccgagcg cagcgactcc tccgcgagtg ccgggaggag gtttgaaccg
     8701 cggcgcacgc catccgtgcg cgcgggttca caccttccag tccgacgccg ccgagtagcg
     8761 tccgcaccga tgaccctcac gatccggctg ctggtcgccc tcgggctgac ctgggcgatc
     8821 ggcttcgaac gcgagctacg tggcgccgca gccggcgacc ggacgtttgc gctgatcggc
     8881 gtgggctcgg cggtgatcgg ctatctcgcc ctcgaccacg cgccgaacgc actcgccggc
     8941 gtggtgaccg gcatcgggtt catcggcgcc ggcctgatcg tccacggcgc cctccccaat
     9001 cggcccggcg cacccgacgg cgcggacgtc gatgccgctg tggatgtcgc cgccggcagc
     9061 ggcgcgatcg gccggatccg gggtgtcacc accgccgcca cgatctacct cgcggcggcg
     9121 gtcggcgctg cggccggtca gggttcgctc ctgttggcgt tcgaggcgac gatcggctgc
     9181 ctgttgcttc tcgaggtacg ccacctgcgg tggctgcggt tcctcgacgg ccgcttctgg
     9241 cgtcatcgcc tgcatcttcc cgacgacgac ccggctgatc tctaggacag ccggtccgac
     9301 ccgtcggcgt taccggccac cgccgcggcg gtcgccgacc tcggaccggg cgacatgggc
     9361 ccgttcggtg gccgacccgg tgcggttgcc gctcatctgc acgagcggtc cgacgggacg
     9421 ggcggcgtcg aatccgttga ggctcgtctg cagcagggaa cgaatgttga ccgcggtcgg
     9481 aagccggtcg agcggcccgg gaagaccgag cagatagccc tgcgctgccg gcaccccgag
     9541 cttcgcgagg agcgagacct ggtggcccga ggtcacgcct tcggccatga cctcgatgcg
     9601 gaggcgggtg gcgagctcgg ccatggcctt cacgacccac gagctgggcg gcatgtcctc
     9661 gccgatgccg gcgatgagcg atgcgtcgag cttgacccgg gtcacgggca ggtcgaccag
     9721 atggccgatc ggagtgttgc gggcgccgaa gtcgtcgagg gcgagaccga gcccgaccga
     9781 cttgagccgt tccaggacca ccgcaaggct tgccggatcg gtgatggctt cggtttcggt
     9841 cagctcgata ccgatcatgg agaccgggag cttcaaccgg gacagctgcg acagcaccca
     9901 gtcggtgccc ccggggcgga tcagatcgtt cggagcgacg ttgaaccaga gcttcgtgtg
     9961 caccggcggg ccactggcga gttcgtcgca caccgcggac acgacgtgct cgaacagctc
    10021 tccggtcaca ccgagccact cggcgatgcc gagccagtcc ttcgccggga tgagtgaacc
    10081 agtgctgtcg acgaggcgtg cgagcacctc ggcgccgata cagcggccgt cgcggagcga
    10141 cacctgcggc tggtaaacga cctggacctt gccgtccatg agggcgtcgc ggacgaaact
    10201 gacgaacgct gcggcctcgg cgcggaacgt gctcgcctgg ccacgacccg tccgcttcgc
    10261 ctggtagagc gccaggtcgg cgttgatgag caggtcggtg accccgccga cgccgcgagc
    10321 cacaccgatg ctggcaccca cggtgaggtc gaggccgccg atctggaagc tctggaggga
    10381 ctggagcagt gagtgcgtca cgtcgtcgac gatcttctgc tcgatcgacg gcaggacgag
    10441 gacgaattcg tcgccgccga gccgggcggc gatgccgtcg tgggtgtcgg cgacctcgcg
    10501 aagtcgctgc gccgacagct gcagcacccg gtcgccgacg gcgtgcccat aggtgtcgtt
    10561 caccggcttg aagtggtcga ggtcgatgtg catgagcacg acctcgggcg accgctcggc
    10621 aagcagggcg acctcctcgt cgaggtggac gcggttgccg agaccggtga gccggtccgt
    10681 cgacgccgcg gcccggagtt cgtcgtcgct gatgcgggag cgcttgcggc gcaacgcgag
    10741 gatcaccgtg gcggctgcga tgcagaggac gatgccgtag aagagcttca ccgacaggtc
    10801 gcggccgttc gcctcgtcga ggcccagctg acgcgcctga ccggtgatga ccttggaacc
    10861 catggcgatg aggtcgcggg agagcgttcc cgccgcagcg tcgacggcag tggcgtcggg
    10921 cgtcgagacc gacccggcat cgacgtcgag gccggcacga gcggccgccc aggtggcagc
    10981 gtcctgcacg agcctcgcct cggtggaggt ggtggcggac gtgaggtcgg cgcggaccga
    11041 ctcgggcacg tccgcggaga tcgcccgcgt gccgaagcgc cacaggtggt cctcggcgtt
    11101 gcgtgccagg tcggcggcga tcgacagtgc atacgagtgg tcaccgccga ggtcggcgcg
    11161 ttcggtctcg atcccgatca gccggtcggc gagttggagg tgggcgacgt cggcggccag
    11221 acggtcggcg aacgcgaggt cgccggccag cggactgagg ttcgatccgt tcgcgacctc
    11281 gccggcgatc tgggtgatgc cgatgtgtag gcgttccaca tcggtcgtgt cctcgggaac
    11341 ggcggtgagc gaatcgtgca gagaacgcac gggctcggcg agatcggggc tcaccttcgc
    11401 caggtcggcg aggtcttcat cgatcgcagc tctgcgggcg acgaacgctg cgacagcgtc
    11461 gtcgcgcgaa cccgacgaag cgagggcggt gtcgagctcg ccgatggcac ccaggatgtc
    11521 gagtcgggcc ttggagaggc ggacccaacc ctgctcggcc acccggtcgt cggcggcgct
    11581 tcgctggagg gacgccatcc cggcgaggag gcctgcggag acgaacaggg ccaccgtgac
    11641 cccgagcgcg actttgctgc tcagccatcg gctgtggtcc ttgaggaccg cggcgatgcg
    11701 gtgaagcgga gatggtgttg gcgccacgta cggactttcg gcacgccctc ccccgcgcct
    11761 gagatttgtc tggacggaat tcgtgccaat ctgcgggcat gccgaagttc agtggccgtt
    11821 ccgaccgcgc cgccgaccgc tccaccgacg gtgcggtcga ccgctccccg tcgcggcagg
    11881 gtggcgcgct gctctcgacc gtcgaccgga tcgtcgagag ccggtacgac tcggcggtcg
    11941 ccctcgtgga cggcatcagg gcccgccccg gccgtccgtc gaccgacgag atcatcgacg
    12001 aactggtgcg gaagtacagc aaggagctcg cagcggtcgc tgcgctgtcg ggcggcgcag
    12061 cggcactccc gggcaccggt accgcggccg ctgtgctcgc gacgggcgcc gacgtggcgt
    12121 tcaccatcgg gaagctcgcc gagatggtgc tcgccatcgg cgtcgcccac ggccacgacg
    12181 cccggtcgat cgaggaacgg cgggcctggg tgctcgccgt gttgtcgatg ggcaagggcg
    12241 ccgtgagtgg cgtcgagggg ttggccgggc gcatcggcgc cgagggcggc gcccggatcg
    12301 tgaccacgat cacgagtacc cagctcgact cggtcaattc ccggctcgca gcgaagatgc
    12361 tcgcccgact cgcctccgag cagagcgcgg cgcggctcgg ccgtctgctt cccttcggca
    12421 tcggcgcggg ggtcggcgcc gccgggaacg tcctgatcgt ccggtcggtc gctcgaaccg
    12481 cccggaagtt cttcgccgtc ggaccatcga cggcgccgat cctcgaggtc gaccccgatg
    12541 tgatcgacgt ggaggttctc gaggactgag tcgggagtag cgtcagcgtc atggccaccc
    12601 cagtactcac cccagcgctc atcgacggca ccgacgtcac cgatggcgag gtcaccgaag
    12661 tccgctcccc cttcgacggg cgggtcgtcg gcctcgtccc gtcgctcacc accgaccacg
    12721 tcgaccgggc ggtcgccgtt gcgctcgaac gccaccgggg gccgcagctc cccgcccacc
    12781 agcgcgccga gatcctcgag cgcgcagcgg tgttgctcac cgaacgcaac gaggagttcg
    12841 ggcaatcgat cagcgccgaa tcggccaagc cgatcaccac cgcccgcatc gaggccagcc
    12901 gcgccgtcga cacgctgagg ttcagcgcgg tccaggctcg cacgctcgtc ggcgagatgg
    12961 tggcgatgga cgccacgccg gcaggggtcg gcaagctcgg ctacgtgaag cgggtgccga
    13021 tcggtgtgat cggcgccatc agcccgttca acttcccgtt gaacctcgtg tgccacaaga
    13081 tcgcaccggc gcttgccgcc ggctgccccg tggtgctcaa gccggcgtcc gcgacgccgc
    13141 tgaccgccct caagatcgcc cgcctgttcg aggaagccgg cctgccgccg ggttggctca
    13201 acgtcgtcac ctgcccgggt tcggtcgccg accgactggt cacccacgac gacgtggcca
    13261 tgatcacgtt caccggctct cccgaggtgg gctggggcat ccgggcccgt ggagcccgca
    13321 agcgggtctc gctcgagctc ggcaacaacg cgccggtcgt cgtcgaggcc gacgcggacc
    13381 tcgaactggc cgccaccaag atcgtcgccg gcggcttcgc gttcagcggc cagacctgca
    13441 tctcggtgca gcgggtgtac gtgcactcct cggtccacga cgagctcctc gacctcgtga
    13501 ccgccaaggt cgaacagctg gtcgtgggcg atcccgccga cgacgcgacc gtcgtctctg
    13561 cgttgatcgc ctccaaggag accaagcggg tcaaggactg gatcgacgag gcggtcgctg
    13621 cgggcgcccg cctcgtggtc ggcggcgacc tcacccccga cgaggtcctc aagccgacgg
    13681 tgctcgacaa cgtgacggcc gacatgaagg tctgctccga cgaggtgttc ggcccgatga
    13741 tcggtgtcgc cagctacgac gactacgagg acgcgctcgc ccgcgccaac gacacccgct
    13801 acggcctcca ggctgccgtc ttcacgaaag acctcgccaa ggcgctccgc gctgccgacg
    13861 tcttggactt cggcggtgta ctcatcaacg agatgccgtc gtggcgggcc gaccagcagc
    13921 cgtacggcgg ggtgcgcgac tccggcaaca cccgcgaggg cccggcgtac accgtccagg
    13981 agatgaccga gcgtcgcgtg atcatcatca caggctgagt gcacaactca cgtcggtggc
    14041 gccggacgtc gtcggcttcg ccgacaaacg aactgtcctt ctgggggcga ggcccgactt
    14101 cgtcgacctc gcccgatccg gctgacggaa cagtcgttcg tcgcgcctag ttcacgatca
    14161 gatcgtcggg gtagagcgcc atctgggaga tgggtctcgg ctgacggcgg agcaccgaac
    14221 gccacaggtc gccgggattg gcgccgaaga ggtcgtcccc accgagatcg tcccggcgcg
    14281 tctcggccac gatccacg
//
LOCUS       DAOSOA010000042        14247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4293_length_14247_cov_5.548055, whole genome shotgun sequence.
ACCESSION   DAOSOA010000042 DAOSOA010000000
VERSION     DAOSOA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14247
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4293_length_14247_cov_5.548055"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /gene="uvrB"
                     /locus_tag="PKY13_04560"
     CDS             complement(<1..1468)
                     /gene="uvrB"
                     /locus_tag="PKY13_04560"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT79231.1"
                     /translation="MTDLTSEHLITEPSIGAPGDGIVRSSKPFKVVSEFQPAGDQPAA
                     IEQLSDGVWRGERFQTLLGITGSGKSATMAWTIEKLQRPTLVLAPNKSLAAQLANEFR
                     EFFPENRVEYFVSYYDYYQPEAYMVSSDTYIEKDATVNDEIDRLRHAATSALLTRRDV
                     IVVASVSCIYGLGSPVEYANKMLVLRVGETHDQRQVLRRLVAMQYERNEMTLIRGKFR
                     VKGDVLEIHPAYDETAVRIEMFGDDIESIWVFDPLTGDRIQQLDEFVLFPATHYATSD
                     ERMNAAIGRIEDELQQRLAWFEKEGKLLEAQRLRMRTQYDLENMREMGFCNGIENYSG
                     PIDGRGPGEPPNTLLDFFPRDYLTIIDESHVSIPQLHGQYEGDRSRKATLIDHGFRLP
                     SAADNRPLRFEEWAERAGQTIFLSATPGPWEREHSGQIVQQVVRPTGLVDPQIVVKPT
                     KGQIDDLLAQINERVVVGDRVLVTTLTKKMAEDLTDYLL"
     gene            1524..1892
                     /locus_tag="PKY13_04565"
     CDS             1524..1892
                     /locus_tag="PKY13_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79232.1"
                     /translation="MTAIEFGTYAAATYFAGFGLGFLVRPAFVTQFGLSWDAAAGKTE
                     VRCYYGAVSIAISAFLLYLVHEDLAREALTGVLFLASAVLVVRILGTIVDGGWSHPYT
                     RSALPIETAFVVALAIVRFR"
     gene            1889..2488
                     /locus_tag="PKY13_04570"
     CDS             1889..2488
                     /locus_tag="PKY13_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79233.1"
                     /translation="MSDASPYERFRRSMDIGYEEWHDGIGYDLEALADATPEDRTAIE
                     TLLVSRAGTDWRDVEALAALGTPGADRALADAFATAGPEIRVAILRDAPHVIPADRVD
                     AELVRLVDECDLGSGMTHVLTLVEDLHPPPVVDALLRGTLDQHRSVPVHYAAMLLYLH
                     GQAAEPFDWEVRPFVLRFSGSGPGERGAAYRELCERIRR"
     gene            complement(2492..3094)
                     /gene="coaE"
                     /locus_tag="PKY13_04575"
     CDS             complement(2492..3094)
                     /gene="coaE"
                     /locus_tag="PKY13_04575"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT79234.1"
                     /translation="MILVGLTGGIGSGKSTVSAALAERGAVVVDADAIVHELQSPGQP
                     VLAAMVERFGEGILHADGSLDRKAVAALVFGDADALKDLGEIVHPPVRDEIFRRVVEQ
                     AATDNVVVLDIPLLTESGWEGLIGTIVVDLDPEIAVARLVEHRDFDEADARARIAKQA
                     TREERLEKAALVVDNSGSLDDLAPQIDACWAWIEGHRTAE"
     gene            3195..5033
                     /locus_tag="PKY13_04580"
     CDS             3195..5033
                     /locus_tag="PKY13_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOT79235.1"
                     /translation="MKTLSPSDDPDELLLGEPPPERPGLLRGRAGREIRRALIDTAVS
                     PDESTAFAPGGIDRLALFTPMVTAVRVATTAISLLLAAPKVLGGDAVITGCAVAIVAY
                     ATLRSIRPIQYSNGIASLNRTLLEALLHVAIIVATGFWSSPFVFTLLSVVVIAGFARG
                     FAFGLRIAISCSIAISFPAIYLSGDGPAALKLAGTWSGLLVLISLVSGYGRKVSGEAD
                     RERDIALDRLGRLADANALLYSLHRVAQTLPSSLDMNEVLTSTVSRLRELIDFDSVVI
                     LLFDDTDSHWQVIRQEGCHLPARLGPTDLPRGLRQAIAESMVICSNELGARDGSGFDE
                     RSGSGLYGALSARGAIMGLVALEHRDPNRFDHRAGGLLAGFVPPLAMALDNARWFARL
                     RTVGADEERTRIARDLHDRIGQSLAYLAFELDRIVSRHQAGDPLGSSLDHLRDDVRGV
                     IREVRDTLYDLRTDVSDSSGLHEILQQYADRVAERTNLQFTIETDDDARLPLLQEREM
                     WRIAQEAITNIERHAKASRVRVTWRCDGGRAAIRISDDGIGFETGRAGRLDSYGMLGM
                     RERASSIGATLEVHSVPGQGTSITCVLDPSVPTTHPDERTKPGARK"
     gene            5030..5680
                     /locus_tag="PKY13_04585"
     CDS             5030..5680
                     /locus_tag="PKY13_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT79236.1"
                     /translation="MNPIRLMLADDHQMLREGLRRSMIDEGFDVVGEAQDGVEAVRLA
                     EELVPDVILMDVSMPNCDGVEACRQVKSHGSVSRVVMLTMHADKEVLTNAIRAGASGY
                     LVKDCSTSEIAEAVRMAAGGETVLSPQLAKSMLEEVRRLDQKARTEEERTVTKREEEV
                     LQLIADGCSTPEVAERLYISQKTVKNHLASIYQKLDARDRTQAVLQAVRMGIVHLH"
     gene            5751..5945
                     /locus_tag="PKY13_04590"
     CDS             5751..5945
                     /locus_tag="PKY13_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOT79237.1"
                     /translation="MEPDDGNDGERIAHRTRVRCDERGANLVEYCLLIVLIVLACLAG
                     VTQFGRAVPSDSFVSVNASI"
     gene            complement(5949..6287)
                     /locus_tag="PKY13_04595"
     CDS             complement(5949..6287)
                     /locus_tag="PKY13_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79238.1"
                     /translation="MRSRPRPLAALLTALAIVASGCGDDPVTRDQYLDHAVEFSTAAK
                     TTEQKASMRKLFDCAWPEVSKDEELLARFMAADTSEPDLSAAMSKLLAPCVGAAAEEA
                     LSTTTTSVAG"
     gene            complement(6302..7084)
                     /locus_tag="PKY13_04600"
     CDS             complement(6302..7084)
                     /locus_tag="PKY13_04600"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT79239.1"
                     /translation="MDKTSKRQVDRDSEQAANRTIRYQVIADDLRRRVVSGEFPAGNL
                     LPSESELSAEYAASRVTVRRALEALRSERLVDSRQGFGWFVAADPLRQELHRLGTIES
                     QLRAAGLASERRILSFSFIEAPPRVRDILGERQVLEVRRLNLADGQPFALVTVWCPEE
                     LGAELSRADVERASFLEQLPVELGGASQTIGAEAAQADVAQLLDVPVGSPLLVAERVT
                     RTVGGRPVLVSRHVFAAHRTEFAVELPAATDVLGPAGLRLVE"
     gene            7151..9436
                     /locus_tag="PKY13_04605"
     CDS             7151..9436
                     /locus_tag="PKY13_04605"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOT79240.1"
                     /translation="MTVEATTPIELVNGVYATLEARLAVGRERLGRSLTLAEKILFNH
                     LDAAQLADPTAPVERAVSYNDLRPDRVAMQDATAQMALLQFMTAGLPQVAVPSTVHCD
                     HLIQAKVGAKTDLLAAEQGNKEVYDFLESVSAKYGIGFWGPGSGIIHQVVLEQYAFPG
                     AMIIGTDSHTPNAGGLGTIAIGVGGADAVDVMTGFPFNVRWPKLIGVHLTGELNGWSA
                     PKDIILKVAEILTVKGGTGAIVEYFGPGAESISCTGKATICNMGAEIGATTSVFGYDE
                     AMSRYLKSTAREAIADAADAVAAHLRADDDVLANPADYFDQVIEIDLSTLQPHINGPH
                     TPDLAREVKDLGAEAREQGWPLEISSALVGSCTNSSYEDITRAASILRHAADNGLSVK
                     VPLLITPGSEQVRATIERDGLLGTFEAAGATVLANACGPCIGQWSRPEADNDHLNTIV
                     TSYNRNFPKRNDGSANTLAFVTSPETVVALALAGTVDFDPINDTLTNAAGEQVKLTVP
                     EGLELPPAGFTPGENTFIAPPEDSSGVEVKVSPTSDRLQLLEPFEPWDGNDYLALPIL
                     LKAKGKCTTDHISMAGPWLKYRGHLENISGNLFLGAVNAFTDAVGEGKDQLDGTTKSF
                     PDIAKHYHEAGQPWIAVGDENWGEGSSREHAAMEPRFRGAKAILVRSFARIHEANLKK
                     QGVLPLTFADPSVYDAIGEDDRISVVGLSELADGKPVDLVLTKPDGTTVSFQANHTMS
                     PEHIEWFKAGSALNIIKASRK"
     gene            9468..9803
                     /locus_tag="PKY13_04610"
     CDS             9468..9803
                     /locus_tag="PKY13_04610"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79241.1"
                     /translation="MNLVVPPGLAEPTAVFWRSVFGMVDLPRQGRSGRPGAWLDGGSF
                     ELHISERDLVGHSDAHVAVAVPDVAAVKTACVVSGAEWEDADALFGVGRGFTRDPGGN
                     RIEVVHDVG"
     gene            9856..9999
                     /locus_tag="PKY13_04615"
     CDS             9856..9999
                     /locus_tag="PKY13_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79242.1"
                     /translation="MRGVLRRDIAEEPEQRHQAELAKSVELNCWNSLDPTAVAVDPVG
                     ALV"
     gene            complement(9996..10760)
                     /locus_tag="PKY13_04620"
     CDS             complement(9996..10760)
                     /locus_tag="PKY13_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79243.1"
                     /translation="MDQERRQARSVREQARVAVPLVIAWGVGLAVMATVAGQSKDRAG
                     QLLMDPSFTLGTRWYTGLVSNLGILAWTVGAAAAFAGAWLCRLGGRSRASKFLVGGGF
                     VGSLLLVDDLFQFHSVLLPSELNAPKFVGEFILGGAMAWWLMSHFREIRRTHVHLLLA
                     AAAGLGLSFVVDAVYAPVPGQGWNIIEDGAKFLGILAWSTYFVVTTRDISRSVFVDAL
                     MTWPDAAYDSVYGALDEFEVDGYAATSAGSGVSSNF"
     gene            complement(10817..11611)
                     /locus_tag="PKY13_04625"
     CDS             complement(10817..11611)
                     /locus_tag="PKY13_04625"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOT79244.1"
                     /translation="MPSSYLVLAATGILLVLVELAIRRWRHLPIDAHETAVSATVGTI
                     WFATKAVGGATGLVAVYAWVFGRWAPWHLDVGNPLTWVAYLVVGDGAYYWVHRAEHRI
                     PFLWASHVVHHSAEDFGFTTAIRMPPTEVLYKPLTGLWAPLLGFPPQIYAPMAAWGLI
                     SGQLQHTQLIGRLGVLDRWLTTPSNHRVHHGSNPRYLDKNFGGQTMIWDRVFGTYEPE
                     TETVRYGATETLLNSGVSGTILGGYPRLFGWGTADGHRAPYGQISV"
     gene            complement(11680..13257)
                     /locus_tag="PKY13_04630"
     CDS             complement(11680..13257)
                     /locus_tag="PKY13_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HOT79245.1"
                     /translation="MAAPVTKSPAKKSPAKKTVAKKAPAKKSPVKKSPAKKAPAAEKA
                     VPAPKRFTGAAFFDLDRTLLAGASGPVFSQALREVGVLTGEAHPVESALFKVFDVFGE
                     NYPTMVLTKQGVRLVKGWDVATVAEAAELAAPRLVDKVLPYAHVEFAEHRTHGRAVVL
                     ATTTPYHLVKPLADLLGFDDVIATRYGTNETGTTFDGTVRGEYIWGKGKSRSVAWWAE
                     EHGIDLDDCHAYSDSYYDVPMLSIVGSPHVVNPDPRMFGIATLRRWPTRYLDAPAGVP
                     KIGGFEPQKLALMFTRSELMPFVRFRSYGKRRIPETGPAIIVGNHRSYFDPLAIGYLL
                     AKRGRPVRFLGKKEVFDAPVVGDFARAMGGIRVERGTGSDAPLQAALEALAAGDMVAM
                     MPQGTIPRGREFFNPKLRGRWGAARLAHESRVPVIPVGLWGTEKVWPRSAKLPNLGNV
                     TDPPVVTIRVGPPVELEYDSLEADTERIMAAIMRLLPPSAREEYEPTDEELRLATPGG
                     HLVPDADHEAERRPGVD"
     gene            13648..>14247
                     /locus_tag="PKY13_04635"
     CDS             13648..>14247
                     /locus_tag="PKY13_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOT79246.1"
                     /translation="MDQRQLRALVAVGEHRSFSAAAKSLHTVQSNISTHVSRLENELG
                     VILVDRATTELTEEGRVVVERARRIDHEFAALASDIAAMRDVVSGTVRAGVIGTTARW
                     LVPPLLNSLKTKYPEVHVVLLDATTSSLALQLIGGQLDMAVVNTPLDAPELRTTPLFD
                     EDRLLVAPAGHPLYDRESVTMADLSEHELLLEAEGTSFRT"
BASE COUNT         2316 a         4967 c         4724 g         2240 t
ORIGIN      
        1 cgagcaggta gtcggtgaga tcctcggcca tcttcttggt gagggtggtc acgaggacgc
       61 ggtcgccgac gacgacccgc tcgttgatct gggccaagag gtcgtcgatc tggcccttgg
      121 tgggcttgac cacgatctgg gggtcgacga gacccgtcgg gcggaccacc tgctgcacga
      181 tctggcccga gtgctcgcgc tcccaggggc cgggcgtggc cgacaggaag atcgtctggc
      241 cggcccgttc ggcccactcc tcgaaccgca gcggccggtt gtcggccgcc gacgggaggc
      301 ggaagccgtg gtcgatcagc gtggccttgc ggctgcggtc gccctcgtac tggccgtgca
      361 gctgcgggat cgacacgtgc gactcgtcga tgatggtcag gtagtcacgg gggaagaagt
      421 cgaggagtgt gttgggcggc tcgcccgggc cgcggccgtc gatcgggccc gagtagttct
      481 cgatgccgtt gcagaagccc atctcccgca tgttctcgag gtcgtactgc gtgcgcatgc
      541 ggagccgttg ggcctcgagg agcttgccct ccttctcgaa ccacgcgagg cgctgttgca
      601 gttcgtcctc gatgcgaccg atggcggcgt tcatgcgttc gtcggaggtg gcgtagtggg
      661 tggcggggaa caggacgaac tcgtcgagct gttggatgcg gtcgccggtg agcgggtcga
      721 acacccagat cgactcgatg tcgtcgccga acatctcgat gcggaccgcg gtctcgtcgt
      781 aggcgggatg gatctcgagg acgtcaccct tcacccggaa cttgccacgg atgagcgtca
      841 tctcgttccg ctcgtactgc atggccacca ggcggcggag cacctggcgc tggtcgtggg
      901 tctcgccgac gcgcagcacg agcatcttgt tggcgtactc gaccggtgag cccaggccgt
      961 agatgcagct gaccgaggcc accacgatga cgtcacgccg ggtcagcagg gccgaggtgg
     1021 cggcgtgccg gagccggtcg atctcgtcgt tgaccgtggc gtccttctcg atgtaggtgt
     1081 cgctcgacac catgtacgcc tcgggctggt agtagtcgta gtacgagacg aagtactcga
     1141 cgcggttctc ggggaagaac tcgcggaact cgttggccaa ctgggccgcc agcgacttgt
     1201 tgggcgccag cacgagcgtc gggcgctgca acttctcgat cgtccacgcc atggtggcgg
     1261 acttgcccga cccggtgatg ccgaggagcg tctggaaccg ctcgccacgc cacacaccat
     1321 cggacagttg ctcgatcgcc gccggctggt cgccggcggg ctggaactcc gacacgacct
     1381 tgaacggctt cgacgagcgc acgatgccgt cgccgggggc gccgatgctg ggttccgtga
     1441 tgaggtgttc ggacgtcaga tcggtcacga gtgcaaggct agacccgacc cctgacagtg
     1501 agaatgtcgg tacgttctcc cccatgaccg cgatcgagtt cgggacctac gccgctgcca
     1561 cctacttcgc cgggttcggc ttgggattcc tggtgcgtcc ggcattcgtc acgcagttcg
     1621 gcctgtcgtg ggacgctgcg gcggggaaga ccgaggtccg ctgctactac ggcgcggtct
     1681 cgatcgcgat ctcggcgttc ctgctctacc tcgtccacga ggatctggcg cgggaggcac
     1741 tcaccggggt gctgttcctg gcatcagcag tgctcgtcgt caggatcctc ggaacgatcg
     1801 tcgacggagg atggagccac ccgtacacgc ggtcggccct gcccatcgag accgcgttcg
     1861 tcgtggccct ggcgatcgtg cggttccggt gagcgacgcg tcgccgtacg agcggttccg
     1921 caggagcatg gacatcggct acgaggagtg gcacgacggc atcgggtacg acctcgaggc
     1981 gctcgccgac gcgacccctg aggaccggac ggcgatcgag accctgctcg tgtcgcgggc
     2041 ggggaccgac tggcgtgacg tcgaggcgct cgcggccctc ggtacccccg gggcggaccg
     2101 ggccctcgcc gacgcgttcg cgactgccgg acccgagatc cgggtcgcca tccttcgcga
     2161 cgccccccac gtgattccgg ccgaccgggt cgatgccgag ctcgtgcgcc tcgtcgacga
     2221 gtgtgacctc ggctcgggga tgacccacgt gttgacgctg gtcgaggacc tccacccgcc
     2281 cccagtcgtc gacgcgctcc tccgcggcac cctcgaccag caccgttccg tgccggtcca
     2341 ctacgccgcg atgttgttgt acctgcacgg ccaggcggct gagccgttcg actgggaggt
     2401 ccggccgttc gtcctccggt tctccggatc cggccccggc gaacgcggag ccgcctaccg
     2461 cgagctctgc gagcgcatcc ggcgctgacc gctactcggc cgtgcggtgg ccttcgatcc
     2521 aggcccaaca cgcgtcgatc tgcggggcca ggtcgtcgag cgaacccgag ttgtcgacca
     2581 cgagcgccgc cttctcgaga cgctcctcgc gggtcgcctg cttggcgatc cgcgcccgag
     2641 cgtccgcctc gtcgaagtca cggtgctcga ccaggcgcgc cacggcgatc tcgggatcga
     2701 ggtcgacgac gatggtaccg atcaggccct cccacccgga ctcggtcagg agcgggatgt
     2761 cgagcaccac cacgttgtcg gtggcggcct gttcgacgac gcggcggaag atctcgtcgc
     2821 ggaccggcgg gtgcacgatc tcgccgaggt ccttgagggc gtcggcatcg ccgaacacga
     2881 gcgccgccac tgctttgcgg tcgagcgaac cgtcggcgtg caggatcccc tcgccgaacc
     2941 gctcgaccat ggcggccagg accggctgac ccggagactg cagctcgtgc acgatcgcgt
     3001 cggcgtcgac gacgaccgct ccccgttcgg cgagcgcggc cgacaccgtc gacttgccac
     3061 ttccgatgcc accggtgagt ccgaccagga tcatgccggg ggacgctacc cgcgtcgcgc
     3121 tgtgcggctc atgttcggcc tggtctcgcc gatgtaaggg gaggccgaac gaactacggc
     3181 cacatgagcc tcccgtgaag acactctccc cctccgacga ccccgacgaa ctgctgctcg
     3241 gcgaaccgcc gcccgagcgt cccgggttgc tgcgcggtcg cgccggccgt gagatccgtc
     3301 gcgccctgat cgacacggcc gtcagtcccg acgaatccac agcgttcgcc cccggcggga
     3361 tcgaccgcct ggcgctcttc acaccgatgg tgaccgccgt acgcgtcgcc accaccgcca
     3421 tctcgctcct tctcgctgcg cccaaggtgc tcggcggtga tgccgtcatc accgggtgcg
     3481 ccgtggcgat cgtcgcgtac gcgacgctcc ggtcgatccg accgatccag tactcgaacg
     3541 gcatcgccag cctcaaccgg acactgctcg aggcgttgct gcacgttgcg atcatcgtgg
     3601 cgacgggctt ctggtcgagc cccttcgtct tcacgctcct cagcgttgtg gtcatcgccg
     3661 ggttcgcccg cggcttcgcc ttcggcctgc gcatcgcgat ctcgtgttcg atcgcgatca
     3721 gttttcctgc catctacttg tccggcgacg gaccggccgc cctcaagctg gcgggcacgt
     3781 ggagcggcct cctcgtcctg atctcgctgg tgtcgggcta cggccgcaag gtgtccggtg
     3841 aagccgaccg cgaacgagac atcgccctcg accggctcgg acggctcgct gatgcgaacg
     3901 cgctcctcta ctcgttgcac cgcgtcgccc agaccctgcc gtcgtcactc gacatgaacg
     3961 aggtgctcac ctcgacggtg tcgcgccttc gcgaactcat cgacttcgac tccgtcgtga
     4021 tcctcctctt cgacgacacc gacagccact ggcaggtcat ccgccaggaa ggttgtcacc
     4081 tgccggcgcg gctcggtccg accgacctcc cccgcggcct ccgccaagcc atcgccgagt
     4141 cgatggtgat ctgctccaac gagctgggcg cccgagatgg cagcggattc gacgaacggt
     4201 cggggtcggg gctctacggg gcgctgagcg cacgcggcgc catcatgggc ctcgttgcgc
     4261 tcgagcaccg cgaccccaac cgcttcgacc accgcgccgg tggtctgctc gccggcttcg
     4321 taccaccgct cgccatggcc ctcgacaacg cccgatggtt cgcccggctg cggaccgtcg
     4381 gtgccgacga ggaacgcacc cgtatcgccc gtgacctgca cgaccggatc ggccagtcgc
     4441 tcgcctacct cgcgttcgag ctcgaccgga tcgtcagccg ccaccaggcc ggcgatccgc
     4501 tcggcagctc gctcgaccac ctccgcgacg acgttcgtgg tgtgatccgc gaggtccgcg
     4561 acaccctgta cgacctccgg accgacgtct cggactcgtc gggtctccac gagatcctcc
     4621 agcagtacgc cgatcgcgtc gccgagcgga ccaacctcca gttcacgatc gagaccgacg
     4681 acgatgcccg gctgccgctc ctccaggaac gcgagatgtg gcgcatcgcg caggaggcga
     4741 tcaccaacat cgagcgccac gccaaggcgt cccgcgtgcg ggtcacgtgg cgctgcgacg
     4801 ggggtcgcgc cgcaattcgg atctccgatg acggaattgg ctttgaaacc ggccgcgccg
     4861 gccgactgga tagttacggg atgctcggca tgcgtgagcg ggcgtcctcc atcggagcaa
     4921 ccctcgaagt ccacagcgtt cccggccagg gaaccagcat cacctgcgtc ctcgacccct
     4981 cagtccccac cacccacccc gacgaacgca cgaagccagg agcaagaaaa tgaacccgat
     5041 ccgactgatg ctggcagacg accatcagat gctgcgtgaa ggtcttcggc ggtcgatgat
     5101 cgacgaggga ttcgacgtgg tcggcgaggc ccaggacggc gtcgaagccg ttcgcctcgc
     5161 cgaggaactc gtgccggacg tcatcctgat ggacgtgtcg atgcccaatt gcgacggtgt
     5221 cgaggcctgc cgccaggtga agtcgcacgg cagcgtgtcc cgggtcgtga tgttgacgat
     5281 gcacgccgac aaggaggtgc tgaccaacgc gatccgcgcc ggcgccagtg gttacctcgt
     5341 caaggactgc tcgaccagcg agatcgccga ggccgtccgc atggccgccg gcggcgagac
     5401 ggtgctgtcg ccgcagctcg ccaagtcgat gctcgaggag gtgcgccggt tggaccagaa
     5461 ggcacgtacc gaggaggagc ggaccgtcac caagcgtgag gaggaggtcc tccagctcat
     5521 cgctgacggc tgctccaccc ccgaggtggc cgagcgcctc tacatctccc agaagacggt
     5581 caagaaccac ctcgcgtcga tctaccagaa gctcgacgcc cgcgaccgga cccaagcggt
     5641 gttgcaggcc gtccggatgg gcatcgtcca cctccattga tcggggccgg ctttgctcgg
     5701 ttcgggcatt tctgcttcag atcatgagcc gttcgaccga tgagcaggga atggaaccgg
     5761 acgatgggaa cgacggggaa cgcatcgcac accgcactcg ggtgcggtgc gacgagcgag
     5821 gggcaaacct cgtcgaatac tgcctcctga tcgtcctcat cgtgttggcc tgcctcgccg
     5881 gcgtcacgca gttcgggcgg gccgtcccct ccgacagctt cgtcagcgtg aacgcctcga
     5941 tctagcgcct accccgccac cgaggtcgtc gtcgtcgaca acgcctcctc ggcggcggct
     6001 ccgacgcatg gcgccaacag cttcgacatg gcagcgctga ggtcgggctc ggaggtatcg
     6061 gccgccatga acctggcgag cagctcctcg tccttggaga cctcgggcca cgcgcagtcg
     6121 aacagcttcc gcatcgaggc cttctgctcg gtggtcttcg cagcggtcga gaactcgacc
     6181 gcgtggtcga ggtactgatc gcgggttacc gggtcgtcgc cgcaccctga cgccacgatg
     6241 gcgagggcgg tgagcagggc ggcgagcggg cgtgggcgtg agcgcatgag cacgacgcta
     6301 tctactcgac caggcgcagc ccagcggggc ccaggacgtc ggtggcggcg ggcagctcga
     6361 ccgcgaactc ggtgcggtgg gcggcgaaca cgtggcgact cacgagaacc ggccggccac
     6421 cgacggtgcg ggtcacgcgc tcggcgacga ggagaggtga cccgaccggc acgtcgagga
     6481 gctgcgcaac gtcggcctgg gcggcctcgg cgccgatggt ctggctcgcc ccaccgagct
     6541 ccaccgggag ctgctcgagg aagctggcgc gctcgacgtc agcccgtgac agctcggctc
     6601 cgagctcctc gggacaccac acggtgacga gggcgaacgg ctgcccgtcg gcgaggttga
     6661 ggcgtcgaac ctcgagcact tggcgttcgc cgaggatgtc tcggacccgc ggcggcgcct
     6721 cgatgaagct gaacgagagg atcctccgtt cgctcgccag gccggcagcg cgaagttggc
     6781 tttcgatcgt gccgagacgg tggagttcct ggcgaagcgg atctgccgcc acgaaccagc
     6841 cgaagccctg gcgggagtcg acgagacgtt cggaccgcag cgcttcgagc gccctccgca
     6901 cggtgacccg actcgccgcg tattctgcgg acagttccga ttccgacggc aggaggttgc
     6961 cggcgggaaa ctcaccggag acgacgcgtc gacgaaggtc gtcggcgatc acctggtacc
     7021 ggatggtccg gttcgccgcc tgctcgctgt cccggtccac ttgtctctta cttgtcttgt
     7081 ccatcgctgg cttgtattct acttgtatct cgtatcaacc gcactcctcc ccgaagcacg
     7141 gagcgttctc atgaccgtcg aagccaccac cccgatcgag ctcgtcaacg gcgtctacgc
     7201 caccctcgag gcacgtctgg ccgtcggccg tgagcgcctc ggtcggtcgc tcacccttgc
     7261 cgagaagatc ctgttcaacc acctcgacgc cgcacagctc gccgacccga ccgcccccgt
     7321 cgagcgtgcg gtctcctaca acgacctgcg tcccgaccgc gtggccatgc aggacgcgac
     7381 ggcgcagatg gcgctgctcc agttcatgac ggccggcctc ccccaggtcg ccgtgccgtc
     7441 cacggtccac tgcgaccacc tcatccaggc gaaggtgggc gccaagaccg acctcctcgc
     7501 cgccgagcag ggcaacaagg aggtctacga cttcctcgag tcggtgtccg ccaagtacgg
     7561 catcggcttc tggggtccgg gctcgggcat catccaccag gtcgtcctcg agcagtacgc
     7621 ctttccgggc gccatgatca tcggcaccga cagccacacc ccgaatgccg gcggcctcgg
     7681 cacgatcgcc atcggggtgg gcggcgcgga tgccgtcgac gtcatgaccg gcttcccgtt
     7741 caacgtccgc tggcccaagc tgatcggtgt ccacctcacc ggcgagctca acggctggtc
     7801 ggcacccaag gacatcatcc tgaaggtcgc cgagatcctg accgtgaagg gcggcacggg
     7861 tgccatcgtc gagtacttcg gtcccggcgc cgagtccatc agctgcaccg gcaaggcgac
     7921 catctgcaac atgggggccg agatcggcgc caccacctcc gtcttcggtt acgacgaggc
     7981 catgagtcgc tacttgaaga gcaccgcccg cgaggcgatc gccgacgccg ccgacgccgt
     8041 tgcggcgcac ctgcgtgccg acgacgacgt cctcgccaat cccgccgact acttcgacca
     8101 ggtcatcgag atcgacctgt cgaccctgca gccgcatatc aacggtcccc acacacccga
     8161 cctggcccgc gaggtcaagg acctcggcgc cgaggcccgc gagcagggtt ggccactcga
     8221 gatcagctcc gccctcgtcg gctcgtgcac caactcctcc tacgaggaca tcacccgggc
     8281 cgcctccatc ctccgccacg ccgccgacaa cggcctgtcg gtcaaggtgc cgctcctcat
     8341 cacgcccggc tccgagcagg tgcgcgccac catcgagcgt gacgggctgc tcggcacgtt
     8401 cgaggcggcc ggtgccacgg tcctcgccaa cgcgtgcggt ccctgcatcg gccagtggtc
     8461 ccgcccggag gccgacaacg atcacctcaa cacgatcgtc acgagctaca accgcaactt
     8521 ccccaagcgc aacgacggct cggccaacac gctcgccttc gtgacctcgc ccgaaacggt
     8581 cgtcgcactc gccctggccg gcaccgtcga cttcgacccg atcaatgaca ccctcaccaa
     8641 cgccgcgggc gagcaggtga agctcacggt gcccgagggc ctcgagctcc cgccggccgg
     8701 gttcaccccc ggtgagaaca ccttcatcgc cccgcccgag gacagctccg gcgtcgaggt
     8761 caaggtctcc cccacctccg accgcctcca gctgctcgag ccgttcgaac cgtgggacgg
     8821 caacgactac ctcgcgctgc cgatcctcct gaaggcgaag ggcaagtgca ccaccgacca
     8881 catctcgatg gccggaccgt ggctcaagta ccgggggcac ctcgagaaca tctcgggcaa
     8941 cctcttcctc ggcgccgtca acgccttcac cgatgccgtc ggtgaaggca aggaccagct
     9001 cgacgggacg accaagtcgt tccccgacat cgccaagcac taccacgagg ccggtcagcc
     9061 ctggatcgcg gtcggcgacg agaactgggg cgagggcagc tcccgggagc acgccgccat
     9121 ggagccccgc ttccgcggcg ccaaggcgat cctggttcgc agcttcgccc gtatccacga
     9181 ggccaacctc aagaagcagg gggtcctgcc gctgacgttt gccgacccgt cggtgtacga
     9241 cgccatcggc gaggacgacc gcatctcggt cgtcggcctg tccgagctcg ccgacggcaa
     9301 gcccgtcgac ctcgtgctga ccaagcccga cggcaccacc gtttcgttcc aggccaacca
     9361 cacgatgagc cccgagcaca tcgagtggtt caaggccggc tcggcgctca acatcatcaa
     9421 ggcgtcgcgc aagtagtcac gtcggccgaa tcgtgcgcct ccatcacgtg aacctggtgg
     9481 tgcccccggg gctcgccgag ccgaccgccg tgttctggcg gtcggtcttc ggcatggtcg
     9541 atctcccgcg ccaggggcgc agcggccggc ccggagcttg gttggacggc ggctccttcg
     9601 agctccacat ctccgaacgc gacctcgtcg gccactccga cgcccacgtc gcggtggccg
     9661 tccccgatgt cgccgccgtc aagacggcct gtgtcgtctc cggtgccgag tgggaggacg
     9721 ccgacgcgct gttcggggtc ggccgcgggt tcacccgaga tcccggcggg aaccgcatcg
     9781 aagtggtcca cgacgtcggc tgatgcccgt caacgagcga acgcgacctc cgcgttctcc
     9841 cgacggggaa cccgcatgag gggtgttctc cgtcgggaca tcgccgagga gccggagcag
     9901 cgccaccagg ccgagctggc caaatcggtg gaattgaact gctggaactc gctcgacccg
     9961 accgccgtcg cggtcgatcc cgtcggcgcc ctggtctaga agttcgacga gacgccggac
    10021 ccggcggacg tcgccgcgta cccgtcgacc tcgaattcgt cgagcgcgcc gtacaccgag
    10081 tcgtaggccg catccggcca cgtcatcagg gcgtccacga acaccgaacg ggagatgtca
    10141 cgggtggtga ccacgaagta ggtcgaccag gcgaggatgc cgaggaactt cgccccgtcc
    10201 tcgatgatgt tccagccctg ccccggaacg ggggcgtaga cggcgtccac gacgaacgac
    10261 aggccgagtc ctgcggctgc agcgagcagc aggtggacgt gggtacgacg gatctcgcgg
    10321 aagtgggaca tcagccacca cgccatcgca ccgccgagga tgaactcccc gacgaacttc
    10381 ggggcgttca gctccgacgg caggagcacc gagtggaact ggaagaggtc gtcgacgagg
    10441 agcagcgaac cgacgaaccc acctccgacg aggaacttgg aggcccggga gcgtccgccg
    10501 aggcggcaca gccaggcgcc ggcgaacgca gcagcagcgc cgacggtcca ggcgaggatg
    10561 ccgaggttgg acacgaggcc ggtgtaccag cgggtgccga gggtgaacga ggggtccatc
    10621 agcagctgtc cggcccggtc cttcgactgg ccggcgacgg tcgccatgac ggcgagacca
    10681 acgccccagg cgatcaccaa cgggacggcg acgcgcgcct gttcccgaac gctgcgagcc
    10741 tgacgacgtt cctgatccat tcactcctca tcggcggtcg tgccgaaaag atgaagactc
    10801 gggaagaact tcccggtcac acgctgatct gcccgtacgg agccctgtgc ccgtcggccg
    10861 ttccccagcc gaagagtcgc gggtacccgc ccaggatcgt gccggacacg ccgctgttga
    10921 gcagcgtctc cgtcgcgccg tagcggaccg tctcggtctc gggttcgtag gttccgaaga
    10981 cccggtccca gatcatcgtc tggccaccga agttcttgtc caggtacctc gggttcgagc
    11041 cgtggtgcac ccggtggttc gacggggtgg tcagccatcg gtcgaggacg ccgagccgac
    11101 cgatcagctg cgtgtgctgc agctggcccg agatcagacc ccaggccgcc atcggcgcgt
    11161 agatctgcgg cgggaagccg aggagcggcg cccacaagcc cgtgagcggc ttgtagagga
    11221 cctcggtcgg tggcatccgg atggcagtcg tgaatccgaa gtcctccgcc gagtggtgca
    11281 ccacgtgcga cgcccacagg aacgggatcc ggtgttcggc acggtggacc cagtagtagg
    11341 cgccgtcgcc cacgacgagg tacgccaccc atgtgagcgg gttgccgacg tcgagatgcc
    11401 acggtgccca acggccgaac acccaggcgt agacggcgac gaggcccgtg gccccgccga
    11461 ccgccttggt tgcgaaccag atcgttccga ccgtcgccga cacggccgtc tcgtgagcgt
    11521 cgatcggcag atgtcgccac cggcggatcg ccagctcgac caggacgagg aggatgccgg
    11581 tggcggcgag gacgagatac gaggagggca tccctccatc atcgggaaca cgatgccacg
    11641 gacccatcgg ggcgaacccc cagagatcgc tggggaggct cagtcgaccc cgggtcggcg
    11701 ctcggcctcg tggtcggcgt cgggaaccag gtggccgccg ggcgtggcca ggcggagttc
    11761 ctcgtcggtc ggctcgtact cctcgcgggc ggacggggga agcagacgca tgatcgccgc
    11821 catgatccgc tccgtgtcgg cctcgaggga gtcgtactcg agctcgacgg gaggaccgac
    11881 cctgatggtc accacgggcg gatcggtgac gttcccgagg ttcggcaact tggccgaccg
    11941 cggccacacc ttctcggtcc cccacaagcc aacggggatc accgggacgc gggactcgtg
    12001 ggcgaggcgg gcggcgcccc agcggccccg cagcttcggg ttgaagaact cgcggccccg
    12061 gggaatcgtg ccctgcggca tcatcgccac catgtcgccg gcggccaacg cttcgagcgc
    12121 cgcctggagc ggagcgtcgg agccggtgcc ccgctcgacc cgaatgccgc ccatggcccg
    12181 cgcgaagtca ccgaccacgg gggcgtcgaa gacctccttc ttgccgagga agcggaccgg
    12241 acgaccgcgc ttggccagca ggtagccgat ggcgagcggg tcgaagtagc tccggtggtt
    12301 gccgacgatg atcgccgggc cggtctctgg gatgcgacgc ttgccgtagc tacggaaccg
    12361 gacgaacggc atgagctcgg accgggtgaa catcagcgcc agcttctgcg gctcgaaccc
    12421 accgatcttg ggcacgccgg caggcgcatc gaggtagcgc gtgggccagc ggcggagggt
    12481 ggcgatgccg aacatgcggg gatcggggtt caccacgtgc ggactgccga cgatcgacag
    12541 catgggcacg tcgtagtagc tgtcgctgta cgcgtggcag tcgtcgaggt cgattccgtg
    12601 ctcctcggcc caccaggcga ccgagcgcga cttgcccttg ccccagatgt actccccgcg
    12661 aacggtgccg tcgaaggtcg tgccggtctc gttggtgccg tagcgggtgg cgatgacgtc
    12721 gtcgaagccg agcaggtcgg ccaacggctt caccaggtgg tagggggtgg tggtcgcgag
    12781 gaccacggct cgaccgtggg tgcggtgctc ggcgaactcg acgtgcgcgt agggcagcac
    12841 cttgtcgacc agccgaggcg ccgccagctc ggcggcctcg gcgacggtgg cgacgtccca
    12901 gcccttcacg aggcggaccc cctgcttggt gaggaccatc gtcggatagt tctcgccgaa
    12961 cacgtcgaac accttgaaca gggccgattc gaccggatgg gcctcgcccg tgagcacgcc
    13021 gacctctcgc agcgcctggg agaacacagg gccgctcgcg ccggcaagca gcgtgcggtc
    13081 gagatcgaag aacgccgccc cggtgaatcg cttcggtgcc ggaaccgcct tctcggccgc
    13141 gggggccttc ttcgccggag acttcttcac tggagacttc ttggcaggcg ccttcttggc
    13201 gacggtcttc ttcgcagggg acttcttcgc tggagacttc gtcacaggag cggccacgag
    13261 gacgacgcta cccgggtccc cggagcgaag cgacggcact ggaagaactc ggtacccctg
    13321 caaggtctac cgccggaggt agcggagcga tcaggaggga gcgcagcgac cgtacctagc
    13381 gaagctggcc tccggccagg aaaaagaccg aaggaccaga ggggggatct ggtccttcgg
    13441 gaaggtcgac ggcagagggg gattccgcct gaagaccgtg gccagagctg ggaggggggg
    13501 gactccgctg tgctccggcg tttgttgttg tggcatcact ataaacactt gcaagcaatc
    13561 tttccagtag ttgcagccga tagctaagat ctctctcaga gatgatcttc gctccgaccc
    13621 cgtacgactg aaggtgcacg cctcaacatg gaccaacgac aactccgagc actcgtcgcc
    13681 gtcggcgagc accgcagctt ctcggcagcg gcgaagtcgc tgcacaccgt gcagtcgaac
    13741 atcagcaccc acgtctcccg tctcgagaac gagctcgggg tgatcctcgt ggaccgtgcg
    13801 accaccgagc tcaccgagga gggtcgtgtc gtcgtcgagc gcgcccgccg gatcgaccac
    13861 gagttcgcgg cgctggcgtc ggacattgcg gcgatgcgcg acgtcgtgtc cggcaccgtc
    13921 cgcgccgggg tcatcggtac gacggcgcgc tggctcgtcc ctcccctcct caactcactc
    13981 aagaccaagt atcccgaggt gcacgtcgtg ctcctcgacg cgaccaccag ctcgctggcc
    14041 ctccagctca tcggcgggca gctcgacatg gcagtggtga acaccccgct cgacgcaccg
    14101 gaactgcgca cgaccccgct cttcgacgag gaccgcctcc tggtggctcc cgccggccat
    14161 ccgttgtacg accgcgaatc cgtcacgatg gccgacctca gcgagcatga gctgctcctc
    14221 gaagccgaag gcaccagctt ccgcacc
//
LOCUS       DAOSOA010000043        14142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4326_length_14142_cov_5.882303, whole genome shotgun sequence.
ACCESSION   DAOSOA010000043 DAOSOA010000000
VERSION     DAOSOA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14142
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4326_length_14142_cov_5.882303"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /gene="argF"
                     /locus_tag="PKY13_04640"
     CDS             complement(<1..979)
                     /gene="argF"
                     /locus_tag="PKY13_04640"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOT79247.1"
                     /translation="MAINLRHRSFVKELDHSPDELRFLVDLAAQLKAAKRAGYERPTL
                     TGRNIALIFEKTSTRTRCAFEVAAHDQGAHVTYLDPSGSQMGRKESTKDTARVLGRMF
                     DGIEYRGFSQDTVETLAQYAGVPVWNGLTDEWHPTQMLADVLTMVEHSNKHLDEIAYC
                     YLGDARNNTARSLLVTGALLGMDVRIAAPAHLWPDADLRATVDKIAADTGARVLVTED
                     LAAAVAGVDYLYTDVWVSMGEPVETWDERIAELLPYQVNEAVVRLTGNPRVLFMHCLP
                     AMHNRDTMIGTELAARTGHSALEVTEDVFESAASIVFDQAENRMHTIKAV"
     gene            complement(981..2240)
                     /locus_tag="PKY13_04645"
     CDS             complement(981..2240)
                     /locus_tag="PKY13_04645"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF002381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HOT79248.1"
                     /translation="MAQFGVHSEVGRLRQVIVHRPDLELTRLTPGNAEQLLFDDILWV
                     KRAREEHDAFAGALRDRGIVVHHFDEVLAETLAVPEGRAYVLDHLCTPDGVGPALVDP
                     LRALGEIADPAELARFLVGGITKHDIDLRGVPSLKWGALTDDDFLLTPLPNHLFPRDN
                     SAWVYGGVSINTMAKTARQRETLHMRAIYRFHPMFSSASFHTWLGGDDVDHQPASIEG
                     GDVHVLGNGAVIVGMGERTTPMAVEQLAAALFAAGQATQVIAAELPRSHAFMHLDTVL
                     TMLDRSTFVAYPYLDTNLRSWTIRPGDGDGDGDGDGDGALAVEANSDMWAAIAAALEV
                     DKVTVLRADEDVRAAEREQWDDGTNFLAVEPGVVVGYNRNVTTNTMLRRNGIEVISIE
                     GSELGRGRGGPRCMTCPVERDAVDGEA"
     gene            2433..3935
                     /locus_tag="PKY13_04650"
     CDS             2433..3935
                     /locus_tag="PKY13_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT79249.1"
                     /translation="MTSTVQRPPIPPADQQRPTPPPGPPTGGSSSGDARGEELGLGSS
                     TALVVGSIIGTGVFTMPAVMAGAGTSSIITLGVIALGAVLLGQLFGQLTKRVPNVDGG
                     LYAYSRHEFGDFAGFLTAWCYWITCWAGNAAIVSSWVLYVESLFGIENPSAWTNLGIA
                     LAGLWIPAAVNLAGVKQMAWLQNVTVVLKFLPLVFVGIVGWFFVTADNFGPFNASGGS
                     LYDGIGIAAGVALFSFIGVECASIAAGRVKNPRRNVGRASVLGTAASGLLYIAVTAVI
                     MGLVPHDELVNDGAPFVAGFKAMFDGAGWAGKAVALIAVVSGLGALNGWTLVTAEMPF
                     AAARDGMFPRAFAKVDAKGTPWFGVIVSTIVASVLMAWSYSGETGLKVFTYLVYLSVV
                     TVAIPYFFSACAQLSYLISRRRKVEGWRLARDIAISVASMLFALWVTMASGYQSVYQT
                     MLLVLVGLPIYAFLKARRERDGLVPEPVDQDDAPTAIDLATVGALVPEAD"
     gene            3939..4724
                     /locus_tag="PKY13_04655"
     CDS             3939..4724
                     /locus_tag="PKY13_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOT79250.1"
                     /translation="MAPRALIRRPGPLLAGGIVTHLERVPVDVDVALEQWEAYTKALA
                     SNGWDLISVPPADDCPDAVFIEDAVVVFHMLDSFRDLAVITRPGADSRKPETIGIEPI
                     VVDQRYSVERIEAPGTLDGGDVLKVGETVYVGRGGRTNGDGIRQLRAILAPHGMNVIA
                     VPITKVLHLKSAVTALPDGTVIGYEPLVDDTRVFDRFRPVPEESGAHVVDLGDGRLLM
                     AADAPRSAELFRDLGYDPVLVDIGEFQKLEGCVTCLSVRLRRP"
     gene            complement(4797..6227)
                     /locus_tag="PKY13_04660"
     CDS             complement(4797..6227)
                     /locus_tag="PKY13_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOT79251.1"
                     /translation="MDTSAPNVGEIGGPTSWAMLDFAPGGIVVAAESGEIVFANRQAR
                     EMFRTGDDGLVGWSVDDLLPTDLRGAHRAHRTRFRARPEVRSMGAGLLLRARRADDTE
                     FHAEISLSPFTSPAGRFVVAAVRDITERVASEDHLHRVLHSLDLSDDAILIFDATTLQ
                     LLYVNAGTERMVGYTRSELLNMTPLHFNPTTAVEDYHAMIAELLADPDRTIRLESVFM
                     RSDGVEIPVEKTYRAGPIGFDGTQWVVAMGRDITDRLAAEQVIRDNQAALLEAERIVM
                     LVDDRERIARDLHDTVIQRLFAAGLGLQSVLRVADDVVRPRLERTIEELDTTIRELRS
                     AIFSLGASTPTMGGLRGRIMDAITEIGSNAAIETRVQFDGPIETLDPAIADHLVPVVM
                     EAMANAAKHAGARNVRVSLTVGDEVVVTVADDGKGIAGEVVGGHGLVNLRERAAGLGG
                     SVDLLPGERCGTELRWAVPAGACADA"
     gene            complement(6305..6811)
                     /locus_tag="PKY13_04665"
     CDS             complement(6305..6811)
                     /locus_tag="PKY13_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOT79252.1"
                     /translation="MASNQQHPVQESVVEQFLAGHRIAVVGASDDPKNFARTIYTELK
                     AHGYEPVPVHPSAVSVDGDVVHHDVASVPGALDGVIIVVSADHAVGVVRECIDCGIDK
                     IWLFQGLGGPGAASDEAVRTCAEAGVDVVPGACPLMFLEPVGWFHRVHRAARRGRHAL
                     VVGSEPVR"
     gene            complement(6887..7789)
                     /locus_tag="PKY13_04670"
     CDS             complement(6887..7789)
                     /locus_tag="PKY13_04670"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOT79253.1"
                     /translation="MRILIALGGNALLPRGAVPDASVQVEHVAAVAPALAAVAAHHQL
                     VITHGNGPQVGLLALESADDHLLTRPYPLDALVAETQGLIGYWLQRSIAAAGLRRPIV
                     SLVTQTLVDPTDPAFDTPTKFVGATYSEGDAHRLAAERGWTVAPDGDRWRRVVASPDP
                     VDVVERPLVEQLLERGTTVVCAGGGGVPVAHSDDGLVGVEAVIDKDSVAALLALQLHS
                     DLLVLLTDVPNVIEGFGTDDARPLGLVTAEEIDSLSFPAGSMGPKVAAASRFARFGRG
                     PAVIGALDDLPQILEGVAGTRVTA"
     gene            complement(7777..8733)
                     /locus_tag="PKY13_04675"
     CDS             complement(7777..8733)
                     /locus_tag="PKY13_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT79254.1"
                     /translation="MDSYLHHLPAGSRPVVVGVDGSSDSTQALLWAAVEAAQRGVPLR
                     IVAAWVVPALAFTTPVVPPTFVDPTIFEAAAEEIVTEAAETVRTRLGAGAPAVETIAV
                     AGEPATVLSESSRHAGMLVVGTHGRGTLARAVLGSVAERCLALSSVPVVVVGEQASAP
                     ADGPVVVGVEDTPAARDALRWAVLEAGRLGRRLVVVHAHEVDSVLRPDLAELPPDVDE
                     VTSKNVRRFLDHLLTEVAVIGGRPRSVAWRVVVGDATHRLVEASADASMLVVGTPRPH
                     GLVERMVGSTGRHCVRTANCPVVVVPAAAIPEVDGPVLECAS"
     gene            8840..9598
                     /locus_tag="PKY13_04680"
     CDS             8840..9598
                     /locus_tag="PKY13_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79255.1"
                     /translation="MHLSDRSGPVRPNGGLGALQAVWTDGFDEMRPLLRTIRDRDAQR
                     RIEILVLRYLGGRAELFDRFADGWAGPPSLAHHDAALRLHRLARDELRRTARSGVLSS
                     EFRDLQARCCRHLELGRVRSVLIGGAPSERSSRIADALGNRHGAVVLRHGDEGPDEAG
                     HRHLLQRAERLLHLGEHVVLDAPWANDPVREAARDSALRSSSDVVELWCHPPVPDGDP
                     HSYDPWPEATVLAPDLGLDHSVDVASAACGWPTP"
     gene            9583..11166
                     /locus_tag="PKY13_04685"
     CDS             9583..11166
                     /locus_tag="PKY13_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT79256.1"
                     /translation="MADTVTLTTGDGTAATPAAVTTGLTDAEADRRHRADGPNRVPPP
                     PPRPAWRRLLGELTHFFALMLWVAGALAVVAGMPQLGVAIFVVIVVNGLFAFAQEGRA
                     EQAAQRLRELLPASVIVVRDGAPRSIDAADVVVGDLVVLAPGDRVPADIELVDADGVS
                     FDTSTLTGESVPVAAGPGDAAFAGTYLVTGSGTGVVTGIAGSTRLAGIAHLAATVQHP
                     RSPLAHEIDRVVRVVAVIAVAVGVVFFGLSLLLGSPARDGLLFAIGVTVALVPEGLLP
                     TMTLSLAMGAQRMAQRNALVRHLEAVETLGSTTFICTDKTGTLTRNQMTVVRVWTPAG
                     DVAIDGPGYEPTGSVTGGVDATSAATRVAEVALAASQGRVRDHDGAWVAVGDPMEAAI
                     DALARRLAGSTDAVATPTILRRNAFDPTRRRESVLVDGALYVKGAPDAVLGRCRDRTA
                     VDTATRALDEMTAAGLRVLAIASRPAQGATDWTSIDEAESDLDLVGLIALHDPPRTNV
                     AAAIAAARSAGGEVGDGDR"
     gene            11141..12211
                     /locus_tag="PKY13_04690"
     CDS             11141..12211
                     /locus_tag="PKY13_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT79257.1"
                     /translation="MKLAMVTGDHPATARAIAAQVGFFVGDELVIEGKDLPTDDDLLG
                     ALIDHDGVVIGRVTPEDKLRIATALQARGHVVAMTVDGANDGPALQRADVGVAMGRSG
                     TDVAREAADLVLLDDDFATIVAAIEQGRATFSNIRRFLTYHLTDNVAELTPFAVWALS
                     GGRFPLALGVLQILCLDIGTDLLPALGLGNEPPSEGTLSRPPEGRHLIDRSLIVRVFG
                     VLGPVEALMEMTAFVVALVAAGWRPGDAFPTGHALLAASGAAFAAVVFGQMANAFACR
                     SATRPVGLRSWRGNRYVTGAVFVELAVLVGFLCIPAVADLLEHAAPPLAGLVAAGAAI
                     PAVLFADRAHKSIRGRRRPTPA"
     gene            12234..13016
                     /locus_tag="PKY13_04695"
     CDS             12234..13016
                     /locus_tag="PKY13_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79258.1"
                     /translation="MTDLLGAVVVDGTGRRVGRVADLTLRLGHGDATVDRLLVRRSGA
                     TTTLPWTAVRRVDVRRISVGTPGEPAVEALRRTDEPLRPDEALASDELLLGRDVLDTQ
                     IVDLDGRRLVRVADVLLAEGPDGSLVAVAAHIGFGAVLQRLGLRRLGRRLHDDAVAWD
                     ALHLTSARGHTVQLATPAAVVHHLTPDDLGVLLDAVTTQAGVEIVRAVHPDLATAALA
                     TSHPHTLRRLNRALHHPDSVPVRRTRRHDGWRRRISPHRMPR"
     gene            13013..>14142
                     /locus_tag="PKY13_04700"
     CDS             13013..>14142
                     /locus_tag="PKY13_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOT79259.1"
                     /translation="MTDIGVTRSETGRRGRVVAVLAVFGPGLLAGLSDDDPAGITTYS
                     VLGAEHGYRLLWVLTASTVALIMFHQLAARMGVATGQGLVGLVRQRSGVRRAALVTGL
                     LLVANIGTTCAEYAGIAAGFELFGVSRYVSVPLAAVTVAAFVMRANFHRVEHVLMAVS
                     AVFLTYIASAVLAHPDWSAAAAGLVVPRLPTTRDAILIATATVGTTLAPWGLSFIQSY
                     AVDKRLTPRDLRYENLDVIVGSVLTGVIGFFVVVACAATLHRTGTPIDEAADAARALE
                     PVAGSMASTLFAVGLIGCALLAAAILPLSTAYSVCEFVGTEAALDDDVRQAPLFYGSM
                     GAVVAISAVIVLLPGISLIPVMVLTQVLNAVLLLPLLVFMVSA"
BASE COUNT         2002 a         5154 c         4854 g         2132 t
ORIGIN      
        1 tgaccgcctt gatggtgtgc atccggttct cggcctggtc gaacacgatc gacgccgccg
       61 actcgaacac gtcctcggtc acctcgagcg ccgaatgccc ggtccgcgct gccagttccg
      121 tgccgatcat cgtgtcgcgg ttgtgcatcg ccgggaggca gtgcatgaag agcacccgag
      181 ggttgcccgt gagccggacg accgcctcgt tcacctggta gggcaacagc tcggcgatcc
      241 gctcgtccca cgtctcgacg ggttcgccca tcgagaccca gacgtcggtg tagaggtagt
      301 cgacgccggc gacggccgca gcgaggtctt cggtgacgag cacgcgggcg ccggtatccg
      361 ccgcgatctt gtcgacggtg gcgcggaggt cggcgtcggg ccacaggtgg gccggggcgg
      421 cgattcggac gtccataccg agcagggcgc cggtcacgag gagcgagcgg gccgtgttgt
      481 tccgggcgtc accgaggtag cagtacgcga tctcgtcgag atgcttgttg gagtgctcca
      541 ccatcgtgag cacgtcggcg agcatctggg tcgggtgcca ctcgtcggtg agaccgttcc
      601 acaccggcac gccggcgtac tgggcgagcg tctcgacggt gtcctgggag aacccgcggt
      661 actcgatgcc gtcgaacatc cgaccgagca cacgagcggt gtccttcgtc gactccttcc
      721 ggcccatctg ggagcccgac gggtcgaggt aggtgacgtg cgccccctgg tcgtgcgccg
      781 ccacctcgaa cgcgcaccgg gtccgcgtcg aggtcttctc gaagatgagg gcgatgttgc
      841 ggccggtgag ggtgggccgc tcgtagccag cgcgcttggc ggccttgagc tgggcggcga
      901 ggtcgacgag gaaccgcagc tcgtcgggcg agtggtcgag ttccttcacg aagctccggt
      961 ggcgcaggtt gatcgccacg tcaggcctcc ccatcgaccg cgtcgcgttc gaccgggcag
     1021 gtcatgcacc gggggccgcc gcgaccgcgt ccgagttcgc tgccctcgat ggagatcact
     1081 tcgatgccgt tgcgccggag catcgtgttc gtcgtgacgt tgcggttgta gccgaccacg
     1141 accccgggtt cgacggcgag gaagttcgtc ccgtcgtccc actgctcgcg ctcggcggca
     1201 cggacgtcct cgtcggcgcg gaggaccgtc accttgtcga cctcgagcgc cgcggcgatg
     1261 gcggcccaca tgtcgctgtt ggcttcgact gcgagcgcac cgtcgccgtc accgtcaccg
     1321 tcaccgtcac cgtcaccggg acggatcgtc cacgaccgga ggttcgtgtc gaggtacggg
     1381 taggccacga acgtcgatcg atcgagcatc gtgagcacgg tgtcgaggtg catgaacgcg
     1441 tgcgagcgcg gcaactccgc agcgatgacc tgggtcgcct gaccggcggc gaagagcgcc
     1501 gcggcgagct gctcgacggc catcggcgtc gttcgctcgc ccatgccgac gatcaccgct
     1561 ccattgccga gcacgtgcac gtcaccgccc tcgatcgacg ccggctgatg gtcgacgtca
     1621 tcgccgccca gccaggtgtg gaacgacgcc gaggagaaca tcgggtggaa ccggtagatc
     1681 gcccgcatgt ggagggtctc gcgctggcgc gccgtcttgg ccatcgtgtt gatggacacc
     1741 ccgccgtaca cccacgccga gttgtcccgc gggaagagat ggttgggcag cggtgtcagc
     1801 aggaagtcgt cgtcggtgag agcgccccac ttcaggctcg ggaccccgcg gagatcgatg
     1861 tcgtgcttgg tgatgccgcc gaccaggaag cgggccagct ccgccgggtc ggcgatctcc
     1921 ccgagggccc ggagaggatc gacgagcgcg ggaccgactc cgtcgggagt acacaggtgg
     1981 tcgagcacat aggcacgacc ctccggcact gcgagcgtct cggcgagcac ctcgtcgaag
     2041 tggtggacga cgatgccgcg gtcgcggagc gcaccggcga acgcatcatg ctcctcgcgt
     2101 gcccgcttca cccacaagat gtcgtcgaac agcagctgtt cagcgttgcc cggggtcagc
     2161 cgagtcagtt cgaggtccgg gcggtgcacg atcacctgcc gcagcctgcc gacctccgag
     2221 tggactccga actgtgccat gggcgtctcc tgtcgtcgat acggcctcca cactccacca
     2281 cgatcaccgc cccaccaagg gccgaaggtc atcaggcggc ccgaacggga tcgggacctt
     2341 tcgggacctc cggccctgcc ccctgtcgcg atcaccggcg catggtggac gtgacccgct
     2401 gcgagagcag caacctcgaa ggggaggaca ccatgaccag caccgttcaa cggccgccga
     2461 tcccaccggc tgatcaacag cgcccgacac caccgccggg cccgcccacc ggtggctcgt
     2521 cctcgggcga tgcccgtggt gaggagctcg gcctgggctc gtcgaccgcc ctcgtcgtcg
     2581 gcagcatcat cggcaccggc gtcttcacga tgccggccgt gatggccggc gccggcacca
     2641 gctccatcat caccctcggc gtgatcgccc tcggggccgt cctgctcggc cagctgttcg
     2701 gccagctcac caagcgggtg cccaacgtcg acggcgggct ctacgcctac tcgcgacacg
     2761 agttcggcga cttcgccggc ttcctgacag cgtggtgcta ctggatcacg tgttgggccg
     2821 gcaacgccgc gatcgtcagt tcgtgggtcc tctacgtcga gtcactcttc ggcatcgaga
     2881 acccctcggc ctggacgaac ctcggcatcg ccctcgccgg gctgtggatc cccgctgccg
     2941 tgaacctcgc gggcgtcaag cagatggcct ggctccagaa cgtcaccgtg gtgctcaagt
     3001 tcttgccgct cgtgttcgtc ggcatcgtcg gctggttctt cgtgacggcc gacaacttcg
     3061 ggccgttcaa cgcctcgggc ggcagcctct acgacggcat cggcatcgcc gccggagtgg
     3121 cgctcttctc gttcatcggc gtggagtgcg cgtcgatcgc cgccggacgg gtgaagaacc
     3181 cccgccgcaa cgtcggtcgg gcctcggtcc tcggcaccgc cgcgagcggg ctcctctaca
     3241 tcgccgtcac cgccgtcatc atgggcctgg tgccccacga cgagttggtc aacgacggtg
     3301 cgccgttcgt cgccgggttc aaggcgatgt tcgacggcgc cggctgggcc ggcaaggccg
     3361 tcgcgctgat cgccgtcgtc tccggtctcg gagccctgaa cggctggacg ctcgtcaccg
     3421 ccgagatgcc cttcgccgcc gcccgcgacg gcatgttccc ccgggcgttc gccaaggtcg
     3481 acgccaaggg caccccctgg ttcggcgtga tcgtctcgac gatcgttgcg tcggtcctga
     3541 tggcgtggag ctacagcggc gagaccgggc tcaaggtctt cacgtacctc gtgtacctct
     3601 cggtcgtgac cgtcgcgatc ccctacttct tcagcgcctg cgcccagctg tcgtatctca
     3661 tctcccgccg ccgcaaggtg gagggctggc ggctcgcacg cgacatcgcg atctcggtgg
     3721 ccagcatgtt gttcgccctg tgggtgacga tggcgtccgg ctaccagtcg gtgtaccaga
     3781 ccatgctcct ggtcctcgtg ggcctgccca tctacgcctt cctgaaggca cgccgtgagc
     3841 gtgacggtct cgttccggag cccgtcgacc aggacgacgc cccgacggcg atcgacctcg
     3901 cgaccgtggg cgccctcgtt ccggaagcag actgagacat ggctccccgc gcactcatcc
     3961 gccgtcccgg tcccttgctg gccggcggca tcgtcaccca tctcgagcga gttcccgtcg
     4021 acgtcgacgt cgccctcgag cagtgggagg cgtacacgaa ggcgctggcg tcgaacgggt
     4081 gggacctcat ctcggtccca cccgccgacg actgccccga tgccgtgttc atcgaagacg
     4141 ccgtcgtggt gttccacatg ctcgactcgt tccgagacct ggcggtgatc acccggcccg
     4201 gtgccgactc ccgcaagccc gagacgatcg gcatcgagcc gatcgtcgtc gaccagcgct
     4261 actcggtcga gcgcatcgag gcaccgggca ccctcgacgg cggagacgtc ctcaaggtgg
     4321 gcgaaacggt gtacgtcggc cggggtggtc gcaccaacgg cgatggcatc cgccagctcc
     4381 gagccatcct ggcgccacac ggcatgaacg tcatcgccgt gcccatcacc aaggtcctcc
     4441 acctcaagtc ggcggtcacg gcccttccgg acggcaccgt catcggctac gaaccgctcg
     4501 tcgacgacac ccgggtcttc gaccggttcc gtccggtccc cgaggagtcc ggtgcccacg
     4561 tggtcgacct cggtgacggg cgcctgctga tggccgccga cgcgccccgc agcgccgaac
     4621 tcttccgtga tctcggctac gaccccgtcc tcgtcgacat cggggagttc cagaagctcg
     4681 agggctgcgt cacgtgcctc tcggtgcgcc tgcggcggcc gtgacaggtc aacacggttt
     4741 cgccgacgag ggacggcgga accgtgtgcc actccccgct ggggaccggc ggcaggtcag
     4801 gcgtcggcgc aggctccggc aggtacggcc caacgcagct cggtgccaca tcgctcaccc
     4861 ggtaacaggt cgacgctgcc gcccagacct gcggcgcgct cacgcaggtt gacgaggccg
     4921 tgccctccga cgacctcgcc ggcgatgccc ttgccgtcgt ccgcgaccgt caccacgacc
     4981 tcgtcgccga cggtcagcga gacgcgaacg ttgcgggcgc ccgcgtgctt cgcggcgttc
     5041 gccatggcct ccatgacgac cggaacgagg tgatccgcaa tggcggggtc gagggtctcg
     5101 atcgggccgt cgaactggac gcgggtctcg atcgcggcgt tgctgccgat ctcggtgatc
     5161 gcgtccatga tccggccgcg gagaccaccc atcgtcgggg tcgacgcccc cagcgagaag
     5221 atcgctgagc gaagctcccg gatcgtcgtg tcgagttcct cgatcgtccg ttccaaccgc
     5281 ggccgcacga cgtcgtcggc gacgcggagc accgactgca ggcccagccc ggcggcgaac
     5341 aaccgttgga tgaccgtgtc gtgcagatcg cgggcgatgc gttcacgatc gtcgacgagc
     5401 atcacgatcc gctcggcctc gaggagcgcc gcctggttgt cgcggatgac ctgctcggca
     5461 gcgagccgat cggtgatgtc gcgccccatc gccaccaccc actgggtgcc gtcgaacccg
     5521 atggggccgg cgcggtacgt cttctcgacc gggatctcga caccgtcact gcgcatgaag
     5581 accgactcga ggcggatggt ccgatcgggg tccgccagga gctccgcgat catcgcgtgg
     5641 tagtcctcga ccgccgtggt ggggttgaag tgcaacggcg tcatgttgag cagctcggac
     5701 cgggtgtagc cgaccatccg ctcggtgccg gcgttcacgt acagcaactg cagcgtcgtg
     5761 gcgtcgaaga tgaggatcgc gtcgtcgctc aggtcgaggg agtggagcac ccggtggagg
     5821 tggtcctcgg aggcgacccg ctcggtgatg tcgcggacgg cggccaccac gaagcgccct
     5881 gcgggcgacg tgaacgggct caggctgatt tcggcgtgga actcggtgtc gtcggcgcgt
     5941 cgcgcccgga gcagcaggcc ggcacccatc gatcgcacct cggggcgggc acggaaccgg
     6001 gtccggtgcg cccggtgcgc cccgcggaga tcggtcggca acaggtcgtc gaccgaccac
     6061 ccgacgaggc cgtcgtcgcc ggttcggaac atctccctcg cctgacggtt cgcgaacacg
     6121 atctcgcccg attcggcggc gaccacgatg ccgcccggag cgaagtcgag catcgcccac
     6181 gaggtcggcc cgccgatttc accgacattg ggagcgctgg tgtccacgag acctccgggc
     6241 gggcgaacgt tccacccgca ggtattctta cccacatcac ccggtgatgc acgttggcga
     6301 cgggtcagcg aacgggctcg gaaccgacga ccagggcatg gcgaccgcga cgtgccgccc
     6361 ggtggacacg gtggaaccac cccaccggtt cgaggaacat gagcgggcac gctcccggca
     6421 ccacgtcgac gccggcctcg gcgcaggtgc gcaccgcctc gtcggaggcc gcgccgggtc
     6481 cgcccagccc ctggaacaac cagatcttgt cgatgccgca gtcgatgcac tcgcggacca
     6541 cgccgacggc atggtccgcg ctcacgacga tgatgacgcc gtcgagcgct ccgggcaccg
     6601 aggccacgtc gtggtgaacg acgtcgccgt cgaccgacac cgcgctcggg tgtaccggca
     6661 cgggctcgta gccgtgtgcc ttcagctcgg tgtagatggt gcgggcgaag ttcttggggt
     6721 cgtcggacgc tccgacgacg gcgatgcggt ggccggcgag gaactgctcg accacggact
     6781 cctggacggg atgctgttgg ttcgatgcca tgtggtgatc gtccgcccgt acccgggccc
     6841 cgcacagagc cgaaggtcac tcgggcccgt ccgcggagcg acccgatcag gccgtgacgc
     6901 gggtgccggc gacgccctcg aggatctgcg gcaggtcgtc gagcgctccg atcacggccg
     6961 gaccccgccc gaaccgcgcg aaccggctcg ccgccgcgac cttcggcccc atcgacccgg
     7021 cggggaagct cagggaatcg atctcctccg ccgtcacgag gccgaggggg cgggcgtcgt
     7081 cggtcccgaa gccctcgatc acgttgggca cgtcggtcag gagcaccagc aggtcggagt
     7141 ggagctgcag cgcgagcagc gccgccacgc tgtccttgtc gatcacggcc tcgacgccga
     7201 cgagcccgtc gtcggagtga gcgaccggca cgccgccacc gccggcgcag accaccgtcg
     7261 tgccgcgttc gagcagctgc tcgacgagcg ggcgttcgac gacgtcgacc gggtcggggc
     7321 tggcaaccac ccgccgccag cgatccccgt cgggggcgac agtccaaccc cgctcggcgg
     7381 cgagccggtg ggcgtcgccc tcggaatagg tcgcgccgac gaacttggtc ggcgtgtcga
     7441 acgccggatc ggtgggatcg accagcgtct gggtcacgag gctcacgatc ggacgccgga
     7501 gaccggcggc cgcgatcgag cgttgcagcc agtacccgat caacccctgt gtctccgcga
     7561 ccagggcatc gaggggatac ggccgcgtca gcaagtggtc gtcggcgctc tcgagcgcca
     7621 gcaggcccac ctggggtccg ttgccgtggg tgatcaccaa ctgatggtgg gcggcgacgg
     7681 cggcaagggc cggggcaacg gctgcgacgt gttcgacctg gaccgaggcg tccgggaccg
     7741 ctccgcgggg caacagcgcg ttgcccccca gggcgatcag gatgcgcact cgagcaccgg
     7801 cccatcgacc tccgggatgg ccgctgcggg cacgaccacc accgggcagt tcgccgttcg
     7861 cacgcagtgg cgaccggtcg aacccaccat ccgctccacg aggccgtggg ggcgaggtgt
     7921 gccgacgacc agcatcgacg cgtcggcgga cgcctcgacc agccggtgag tggcgtcacc
     7981 gacgacgacg cgccacgcca ccgaacgcgg ccggccaccg atcacggcca cctccgtcag
     8041 caaatggtcg aggaaccgtc gcacgttctt ggacgtcacc tcgtcgacgt cgggaggcaa
     8101 ctcggcgagg tcggggcgca ggaccgaatc gacctcgtgg gcatggacca cgaccagccg
     8161 acgaccgagg cgaccggcct cgaggaccgc ccaccgcagc gcgtcgcgcg ccgcaggggt
     8221 gtcctccaca ccgacgacca ccgggccgtc ggcaggagcg gacgcctgtt cgccgacaac
     8281 gacgaccgga accgacgaga gcgccaggca ccgttcggcc accgacccga gcaccgcccg
     8341 agcgagcgtg ccccgcccgt gagtccccac gaccagcatc ccggcgtgcc gcgaactctc
     8401 gctgagtacc gttgcaggct caccggcgac cgcgatcgtc tcgacggcgg gagcgcccgc
     8461 gccgagtcgc gtccggaccg tctcggcggc ctcggtcacg atctcctcgg ccgccgcctc
     8521 gaagatcgtc ggatcgacga aggtcggcgg cacgaccggg gtcgtgaacg cgagcgcagg
     8581 aacgacccac gccgcaacga tccggagtgg cacaccccgt tgcgccgcct cgacggcagc
     8641 ccacaggagc gcctgggtcg agtcggacga cccgtcgacg ccgacaacga ccgggcggga
     8701 acccgcggga aggtggtgaa ggtaggagtc catggctccc atcgtcgaca tggccgtagg
     8761 accgggacag ggccaaaggt caccacggcc gtgaccttcg accctgccga agcgccgcag
     8821 ttccgacgac catgcacgaa tgcatctctc cgaccggtcc ggaccggtcc gaccgaacgg
     8881 cggcctcgga gcgctccagg ccgtgtggac cgacggcttc gacgagatgc gccccctcct
     8941 gcggacgatt cgggaccgcg acgcccagcg ccggatcgag atcctggtgc tccgttacct
     9001 cggtggacga gccgagctct tcgaccggtt cgccgacggc tgggcgggac cgccgtccct
     9061 cgcccaccac gacgctgcgc tgcgcctgca tcggctcgcc cgcgacgagc tccgccggac
     9121 ggcgcggagc ggcgtgctca gcagcgagtt cagagatctc caggcccggt gctgccgcca
     9181 cctcgaactc ggccgggtcc ggtccgtgct gatcggcggc gccccctccg aacggtcgag
     9241 ccggatcgcc gacgcgctcg gcaaccgaca tggggccgtt gtgctccgcc atggcgacga
     9301 gggtcccgac gaggcgggcc accgccacct cctgcaacgc gccgagcggt tgctgcacct
     9361 cggcgaacac gtggtgctcg acgccccctg ggcgaacgac cccgtccgtg aggcggcgcg
     9421 ggattcggca ctccgctcgt cgtccgacgt cgtcgagctg tggtgccacc cgccggtccc
     9481 cgacggagac ccccacagct acgacccctg gccggaggcg accgtcctcg cccccgacct
     9541 cgggctggat cactcggtcg acgtggcgtc cgcagcgtgc ggatggccga caccgtgacc
     9601 ctcacgaccg gcgacggtac ggcggcgacg ccggcagccg tcacgaccgg gctgaccgac
     9661 gccgaggcgg atcgccgcca ccgcgccgac gggcccaacc gtgtgccccc acccccgccg
     9721 cgaccggcgt ggcgacggct cctcggcgaa ctcacccact tctttgcgct catgctctgg
     9781 gtcgccggcg ccctggccgt ggtcgcaggg atgccacagc tcggggtcgc catcttcgtc
     9841 gtcatcgtcg tcaacggact gttcgccttc gcccaggagg gtcgggccga gcaggcggca
     9901 caacgccttc gagagctcct cccggcgagc gtcatcgtgg ttcgagacgg tgcgccgcga
     9961 tcgatcgacg ccgccgacgt cgtcgtgggc gacctcgtgg tgctcgcccc gggcgaccgg
    10021 gtccccgccg acatcgagct cgtcgacgcc gacggggtgt cgttcgacac ctcgaccctc
    10081 accggtgaga gcgtgccggt ggccgccggc cccggtgacg ctgcgttcgc cggcacctac
    10141 ctcgtcaccg ggtcgggaac cggcgtcgtg accgggatcg ccgggagcac acggctggcc
    10201 gggatcgccc acctggccgc cacggtccag cacccgcgga gcccgctcgc ccacgagatc
    10261 gatcgggtgg tcagggtcgt cgccgtgatc gccgtcgcgg tcggcgtcgt cttcttcggg
    10321 ttgtccctcc tcctcggctc accggcccgc gacggcctgc tgttcgccat cggggtcacg
    10381 gtggcgttgg tgcccgaggg gctcctgccc accatgacgt tgtcgttggc gatgggcgca
    10441 cagcggatgg cgcagcgcaa cgccctggtc cgacacctcg aagccgtcga aacgctcggc
    10501 tcgacgacct tcatctgcac cgacaagacg ggcacgctga cccgcaacca gatgaccgtc
    10561 gtgagggtgt ggacccccgc cggtgacgtc gcgatcgacg ggccaggcta cgagccgacg
    10621 ggttcggtca cgggcggggt cgacgcgacc tcggccgcga cgcgtgtggc cgaggtcgcg
    10681 ctcgccgcgt cccagggccg ggtacgcgac cacgacgggg cgtgggtcgc agtgggcgac
    10741 ccgatggagg cggccatcga cgcgctggcg cggcggctcg ccggatcgac cgatgccgtc
    10801 gccacgccga cgatcctgcg ccgcaacgcg ttcgacccaa cccggcgccg ggagtccgtg
    10861 ctcgtcgacg gggccctgta cgtgaaagga gcgccagatg cggtgctcgg ccggtgccgc
    10921 gaccggacag cggttgacac ggcgacccgg gccctcgacg agatgaccgc cgccggtctc
    10981 cgggtgctgg ccatcgcgtc gcgcccggcg cagggagcga ccgactggac ctcgatcgac
    11041 gaggccgagt ccgatctcga cctcgtgggc ctgatcgcac tccacgaccc gcctcggacc
    11101 aacgtcgccg cagcgatcgc cgccgcccgc tcagccgggg gtgaagttgg cgatggtgac
    11161 cggtgaccac cctgcgacgg cgcgggcgat cgccgcccag gtggggttct tcgtcggcga
    11221 cgaactcgtg atcgagggca aggacctgcc gaccgacgac gacctgctcg gcgcgttgat
    11281 cgaccacgac ggagtggtca tcgggcgcgt cacacccgag gacaagctgc gcatcgcgac
    11341 ggcgttgcag gcccgcggcc acgtcgtcgc catgacggtc gacggcgcca acgacgggcc
    11401 cgccctgcag cgggccgacg tgggcgtggc catggggcgt agcggcaccg acgtcgcccg
    11461 cgaggcggcc gacctggtgc tcctcgacga cgacttcgcg acgatcgtcg cggcgatcga
    11521 gcagggtcgg gccacgttct ccaacatccg ccggttcctc acctatcacc tgaccgacaa
    11581 cgtggcagag ctcacgccgt tcgccgtctg ggcgctgtcg ggcggccggt tcccgctcgc
    11641 cctcggggtg ctgcagatcc tctgtctcga catcggcacc gatctccttc cggcgttggg
    11701 cctcggcaac gaacctccct cggagggcac cctcagccgg ccgcccgagg gccgccacct
    11761 gatcgaccgt tcgctgatcg tccgggtgtt cggggtgctc gggcccgtcg aggcgttgat
    11821 ggagatgacg gcgttcgtcg tcgcgctcgt cgccgccggg tggcgaccgg gcgacgcctt
    11881 ccccaccggt cacgcgctcc tggcagcgtc gggcgcagcc ttcgccgcgg tggtgttcgg
    11941 gcagatggcc aacgcgttcg cctgccgcag cgccacccgg ccggtgggcc tgcggtcgtg
    12001 gcgaggcaac cggtacgtga ccggcgctgt cttcgtcgag ctggcggtcc tggtcgggtt
    12061 cctgtgcatc cccgccgtcg ccgacctgct cgagcatgcc gctcccccgc tcgccggcct
    12121 cgtcgcagcc ggcgccgcca tcccggcggt actgttcgcg gatcgtgccc acaagtcgat
    12181 tcgcggccgc cggcgcccga ccccggcctg acgtggaccc gcgggtccac cggttgaccg
    12241 acctcctcgg cgccgtcgtc gtcgacggga cgggccgacg cgtcggccgg gtggccgacc
    12301 tgacgctccg tctcggacac ggcgacgcga ccgtcgaccg gttgctcgtc cgccgcagcg
    12361 gggccacgac gacgctcccg tggacggcag tgcgacgcgt cgacgtccga cgtatcagcg
    12421 tcgggacccc gggcgagccg gctgtggagg cgctgcgccg aaccgacgaa ccgctcaggc
    12481 ccgacgaggc actcgcgtcc gacgaactcc tgctcggccg cgacgtgctc gacacccaga
    12541 tcgtcgacct cgacggccgc cgactggtcc gggtcgcgga cgtcctgctc gccgaaggtc
    12601 ccgacggatc gctcgtcgcc gtcgccgccc acatcgggtt cggagccgtg ctccagcgcc
    12661 tcggtctgcg gcgactgggc cgccggctcc acgacgacgc ggtggcgtgg gacgcgctgc
    12721 acctcacgtc ggcgaggggc cacaccgtcc agctcgccac cccggccgcc gtggtgcacc
    12781 acctgacacc cgatgacctc ggtgtcctgc tcgatgccgt gacgacccag gcgggtgtgg
    12841 agatcgtccg cgccgtccac cccgacctgg cgaccgccgc tctggcgacc tcgcaccccc
    12901 acacgctccg gcggctcaac cgggcgctcc atcacccgga ctccgtgccc gtccggcgta
    12961 cccgccgcca cgacgggtgg cgacgtcgca tctcccccca tcggatgccg cggtgaccga
    13021 catcggcgtc acccgttcgg agaccggccg ccgcggccgg gtcgtcgccg tgttggccgt
    13081 gttcggcccc ggcctgctcg cggggctgtc cgacgacgac cccgccggga tcacgacgta
    13141 ctcggtgctc ggcgccgagc acggctaccg gctcctgtgg gtgctcacgg cctcgacggt
    13201 cgccctcatc atgttccacc agctcgccgc ccgcatgggt gtcgcaaccg gccagggcct
    13261 cgtcggactg gtccggcaac gctccggagt ccgccgcgcc gcgctcgtga ccggccttct
    13321 gctcgtcgcc aacatcggca ccacctgcgc cgagtacgcc gggatcgccg ccgggttcga
    13381 gctgttcggg gtgagccgtt acgtgagcgt gccgctcgcc gccgtgaccg tcgcagcgtt
    13441 cgtgatgcgc gcgaacttcc accgggtcga gcacgtgctg atggcggtca gcgccgtgtt
    13501 cctgacctac atcgcctccg cggtgctggc ccaccccgac tggagcgctg ccgcagccgg
    13561 cctggtcgtc ccccggctcc ccacgactcg cgatgccatc ctcatcgcga cggcaacggt
    13621 cggcaccacc ctggcgccct ggggcctgag cttcatccag tcgtacgccg tcgacaaacg
    13681 cctcaccccg cgtgatcttc gctacgagaa cctcgacgtg atcgtcgggt cggtgctcac
    13741 gggggtgatc ggcttcttcg tcgtcgtcgc gtgcgcagcg acgctgcatc gcaccggcac
    13801 gccgatcgac gaggcagccg acgccgcccg agcgctcgag cccgtcgccg gctcgatggc
    13861 atcgaccctg ttcgccgtgg ggctcatcgg gtgtgcgctg ctcgccgcgg cgatcctgcc
    13921 gctgtcgacg gcgtactcgg tgtgcgagtt cgtggggacc gaggcggcac tcgacgacga
    13981 cgtccgccaa gcgcccctct tctacggatc gatgggcgcc gtcgtggcga tctcggcggt
    14041 gatcgtgctg ctgccgggca tctcgctgat cccggtgatg gtgctcaccc aggtgctcaa
    14101 cgccgtgctg ctgctcccgc tcctcgtgtt catggtgtcg gc
//
LOCUS       DAOSOA010000044        14085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4348_length_14085_cov_5.997292, whole genome shotgun sequence.
ACCESSION   DAOSOA010000044 DAOSOA010000000
VERSION     DAOSOA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14085
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_14085_cov_5.997292"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="PKY13_04705"
     CDS             complement(<1..1591)
                     /locus_tag="PKY13_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79260.1"
                     /translation="MTGWPFVGREETVDHVTGLLQDPDVAAVLLEGPAGIGKSAIAAH
                     VATTLAAVSGASVVKVRGPDTLARFDGIGLLRPEVLDVTSIDPTGHVQAVLDRWMIDA
                     PDLVWVDDVGHLDAGSAVVLLHVARLGASKLLLTSRTGAPSSEAVAQLEHLGVLRRLD
                     VAPLDLAACGDLLHAALGEPAGNDIARRVLERSHGNALVIRELVRAAIADGSLVRGRD
                     AWRWDDGDGLVPNAAALTAHHMQSLSPPARRLVDLLVIAGELELADLAGADPAFATAV
                     DELVDADIGTIDGSAVRLAHPLFAEAARVDLDPESRIERLTELIELTAGSIQRPALRL
                     RHLRWRQEVGVHIDPSELAEGTHLAMTMFDTATAIDLGQAAIDAGRTDVALPLAMALL
                     YDGRLEDAAVAAKAAVEGALDEATRTFASVTGSLARAYRSGFDSSIAADHEELLDSTT
                     DPSLAAFVRSEWGSALAFSGQLADAVAAAADGVATGQWWERVPFVRGWAGAMTALGRT
                     TEVLDVVGGLVAEAGSHAGRSV"
     assembly_gap    1786..1885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1886..2074
                     /locus_tag="PKY13_04710"
     CDS             <1886..2074
                     /locus_tag="PKY13_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT79261.1"
                     /translation="REIAEYVVEGWRNREIADRLCVSVRTVEGHVLRLCRKLGVTDRS
                     QVEARHLDPAGGGGAAGP"
     gene            2169..3593
                     /locus_tag="PKY13_04715"
     CDS             2169..3593
                     /locus_tag="PKY13_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HOT79262.1"
                     /translation="MSMTTNHPPHRRLPVGRIVLVVLGCVFVPLALALLGGGTVLTWA
                     YQTQRDASGYFTSPSERLETLDYAIVSERVDLDLDSGDYLGVGQLGTVRIRLDSTNER
                     QVFIGIGPRRAVKDYLRGVARAEIADVSTDPFSVEYRHLGDGAPTGPPGDESFWQAQA
                     EGPGPQTLTWPVESGDWVVVVMNRDATAGVGVDAAVGFKADWIRPLGIGLLVAGGLAL
                     IIAVAALVVGIVGLRHHAASDETGGTHVHTGSGPVRLTGRLDAELSRWLWLVKWFLVI
                     PHLVILAFLWLAFAVLTVVAFFAILLTGRYPRGIFDFNAGVLRWSWRVSYYAFGVAGT
                     DRYPPFSLGEHPDYPARLDIAPPDQLSRGLVLVKWWLLAIPHYLIVGLMVGTTVSSRS
                     GDRWVDVPVTGLISWLVLVAVVTLLFTGRYPSGIHRLVVGLHRWVFRVVAYAALMTDE
                     YPPFRLDQGDEEPSVDSELGAPPS"
     gene            3590..4240
                     /locus_tag="PKY13_04720"
     CDS             3590..4240
                     /locus_tag="PKY13_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79263.1"
                     /translation="MRRCSVERVATAGAIAVLAAVGRRRHLTWGSTPDEVARALPGDE
                     LVRSPHLTSTRSITVRAAAGDVWPWLAQMGQGRGGLYSYDWLENLAGCQMHSAHRVVP
                     EWQSVEVGDPFRLHPDLALRVARIDPGESLVVEGGVSPTGQETSGSDAPYDFSWAFVL
                     VPVGADATRLIIRERYRYHAAWVRLMVEPISVISCVMTERMMRGIRDRAEGTRSGS"
     gene            complement(4284..4634)
                     /locus_tag="PKY13_04725"
     CDS             complement(4284..4634)
                     /locus_tag="PKY13_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79264.1"
                     /translation="MTLAWEQLVIDADDPDALARWWADALGWTEIIVDQDGDVEIRSG
                     PDRFPGVLFQHVPDPKTVKNRFHLDVRPDDRDAEVERLVALGATHVDIGQGTPSWVVL
                     ADPEGNEFCVLRSR"
     gene            4777..6171
                     /locus_tag="PKY13_04730"
     CDS             4777..6171
                     /locus_tag="PKY13_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT79265.1"
                     /translation="MEANTSSIQAEMAEVLAPLRSVLARLAPNDIPLPEVAGIWQLFS
                     QVRRLAGNGETLLAGRVAEAREWARSGYATPEEWMAAKAGTSVGEARGQIATSEQLGQ
                     GLDETAERMRQGRLSPQQAGIVADAAAVNPDAELELCDRAERDSNKNLSDEARRRKAQ
                     RSDGDDRARRHHRERRARRYSDADDKSHLHVEGPTSKLAEFFKALDRETDRRYREATK
                     QGRREGRDAYAFDAFLSLVTQGIAGDAATSSKPKHLAMIRVDLDALVRGHVEGDETCE
                     IAGFGAISVAEARALLSESILYLVCTKGVDVVNLTHLGRGPNRVQELALLWASQTCQV
                     AGCYRTHTQYDHRIDFAETRHTRVDETDLLCVHHHKMKTFKGYALVAGTGKRRFVPPG
                     DPDHPATSRSGPGSPRSDGSSPGPAQPPPPEPSQEPPPPSESIEERIGRLRASAQARR
                     RRAAGQGDLFGLAE"
     gene            6349..7497
                     /locus_tag="PKY13_04735"
     CDS             6349..7497
                     /locus_tag="PKY13_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT79266.1"
                     /translation="MSTDEQILGRTEVDDLEAIISMYNTDVTEALHVVADNAPAIFTW
                     DYEKGARPQLNKLYEKAKHAQWNGETDLDWSIEVDQEYFAKLLSDMRAMQMAEAGITF
                     DGTPFANWGDKEWLAHSMESQNWSLSQFMHGEQGALICTAKIVETVPWIDAKYYAATQ
                     VMDEARHVEVFAKYLDTKLNGHYPINTHLRMLLDDIINDSRWDMTYLGMQIMVEGLAL
                     AAFGMAMQTTPDPLLKQLLRYVMSDEARHVAFGVLSLKEFYDGLTDAELLERQEFAFE
                     AAVRMRDRFLQQEVWDRLGVNVKDAVKLFDVPAEVKNRDPFQQLLFSKIVPNCKKLGL
                     LDANDGWLRKRFDTLGVTQFEDWADTGEEYLEMDAVSQDRLAAQAAAG"
     gene            7597..8070
                     /locus_tag="PKY13_04740"
     CDS             7597..8070
                     /locus_tag="PKY13_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT79267.1"
                     /translation="MTTLELDRTTLDLDPTAPTAGLRRHVPRLVRYGTVSIVSTAFSL
                     TILTVLVASGALSAGWGNLVATLCGAVPSFELNRRWVWPGGGRPRLAQAVPFVAMSLA
                     GLALSAVLVAVTEGWSSEWSDGSRALLADGSNMAAFGSLWLVQFVICDRHLFGRR"
     gene            8138..8410
                     /locus_tag="PKY13_04745"
     CDS             8138..8410
                     /locus_tag="PKY13_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79268.1"
                     /translation="MAHEREPWFHDEHGNLVVVQAPNLPILVWLGAVVLRTLVHGGAP
                     ERLADAVGFGALFTWAYLEVAQGSARIRRILGAVVLVAMVVGRVRG"
     gene            complement(8442..9530)
                     /locus_tag="PKY13_04750"
     CDS             complement(8442..9530)
                     /locus_tag="PKY13_04750"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT79269.1"
                     /translation="MSVAGWFPDPSDPGLLRYWDGAAWTDEVRPMPSTSVLPPMPPFG
                     VDPASTPSPPPAPSTFGPPVAAWEDTAAPPPTPRSMAPTSTARRRQGRRRPVWRIALF
                     GVLAIGVVAGAAFGVWKFADTVGGAGEFDRPGAVVSAGDDDGSAAEADVITALLRHGI
                     TCASDAAALPHADLVAAVERIAADSSTGPADLRDATDDFVRAVTSLDPTIAATLTPCG
                     RSLLVVTAGTARYGFFDGAAPPAVAAVSDLGLRLVSLAPPTMTDTIALVEDRGETCPL
                     PGRTTSKDVLLAELLPRLAPADGSVPTPATTAEKVAVDGLPDQEIVVRNCTHTRVLGA
                     DAWAEVVILSPDSEVTAASLGHVDVVGP"
     gene            complement(9638..10540)
                     /locus_tag="PKY13_04755"
     CDS             complement(9638..10540)
                     /locus_tag="PKY13_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOT79270.1"
                     /translation="MQRPGIRDGMAQDLRNSYRIAWLFEKTPWGKASGARDIIVDLHA
                     VTFQELNPVREAWQQDRFRDRIGAFGDNTMITAPEVYWDHCGPRTICMERVRGVPMDH
                     FDELANRGLDLQLVVRRGAKVWAEAAMLHGPFHGDMHAGNIWVLDDGRGCFLDFGIMG
                     ELDQEWRDLLQDLFYTCAFDLDFSRVASAYRRVGAIPEEFGDEDQLAAFLTNVLGAML
                     SSGFGGVDIAQLVSESVKMLKAYDASIPRELVLIAKQLLYIDRYTKHLAPDYSLTADP
                     FIVKNVFPEEAAKKAADLGAEFPA"
     gene            11246..12160
                     /locus_tag="PKY13_04760"
     CDS             11246..12160
                     /locus_tag="PKY13_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79271.1"
                     /translation="MAGRTAERAARRARRRFRRLYRVAVVLIVAFGVILVRNRGDDPV
                     WTAVDTAGQLLAAVLAAAAGAVTARRSSGRARWGWSTIAAGCAAWAVGRGIWLVSAMV
                     LHHNVPAPGIADIGFVAAPVLWTAGILMLVDTPPGTRSRTSGLLEGLMIAGGVFLPTW
                     ALALAPVVRESGGPGPAQIVDLARPVLDLVVLASLLFAFPRCRRHARGSLALLAIGVA
                     AIATADSGFWYLRTVDSYIGTNLFDLGWFAGFLFIGLGSLRPSAGTPTGTSAGTQAGA
                     PALDPEFRDSWRLLRAAARTLPTVTSRG"
     gene            complement(12144..13805)
                     /locus_tag="PKY13_04765"
     CDS             complement(12144..13805)
                     /locus_tag="PKY13_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOT79272.1"
                     /translation="MSRTTRAALYSRVSTDEQATHGTSLADQQRRAAAYCETSGWNVV
                     GQFVDDGVSGATIHRPALQGLLQSAREGSFDVVVVTDPDRLSRDLVDGLVLERELARA
                     EVGVVYLVQPTMSTLERQLRGVIAEEERRKIRDRMVRGIRAVAEAGYWPGGPPPFGYT
                     VVRDEGEAHSRLALNPQEVEVIASVVDQFIDDRRSTLKIADDLNDRDVPTPGATRRLS
                     NPGTGRWTHRRVRDMLRTATGISGTWTYTTDTHTITLSIPAIISEPRHEQLRQRLAET
                     STGPGATLARHRYLLGGRLTSPCGSNMQGTGRPDRDARVYRCANNLAERGPARCDCPR
                     IHAAGVEARVWSATHEMLTDPERLLTLAGIADSAHQPAGADDLTALTRKIRRIESALG
                     SSIAQLVTSGTDPVAIRHATRQLEEELERLKMHRDRVVGWAAARDTQSDHARRLLTLA
                     DTARDALDHAADDLRLNIIQLLDIRVRVVGTMPCHECSGKGLIKASPETATTGRGRTG
                     RVCPTCARFRRIPSIEISGAIPDAGSIEDARAATSAMPFRLIHGL"
BASE COUNT         2049 a         4733 c         5022 g         2181 t
ORIGIN      
        1 cgacggaccg accggcgtgg ctgccggcct cggcgaccag gccgccgacg acgtccagta
       61 cctcggtcgt ccggccgagc gcggtcatcg ccccggccca cccgcggacg aacgggacgc
      121 gctcccacca ctggccggtc gcgacgccgt cggctgcggc ggcgacggcg tcggcgagct
      181 gcccgctgaa ggcgagggcc gagccccatt cgctgcggac gaacgcggcg agcgacgggt
      241 cggtcgtcga gtccagcaat tcctcgtggt ccgccgcgat cgacgagtcg aagccggacc
      301 ggtacgcacg ggcgaggctg ccggtgaccg aggcgaacgt ccgggtggcc tcgtccaacg
      361 caccctcgac cgccgccttg gccgccaccg cggcgtcctc gagacggccg tcgtagagga
      421 gggccatggc gagcggcagc gccacgtcgg tccgcccggc gtcgatggcg gcctgtccga
      481 ggtcgatcgc cgtcgccgtg tcgaacatcg tcatcgccag gtgcgtgccc tccgccagct
      541 cggacgggtc gatgtggacg cccacttcct gtcgccaacg caggtggcgg aggcggaggg
      601 ccggccgctg aatggaaccg gccgtgagct cgatgagctc ggtgaggcgc tcgatccgtg
      661 actccggatc cagatcgacg cgggcagctt cggcgaagag agggtgggcg agccggaccg
      721 cggacccgtc gatggtgccg atgtcggcgt cgacgagttc gtcgacggct gttgcgaagg
      781 ccgggtccgc gcctgccagg tcggccagtt cgagttcgcc ggcgatgacc aggaggtcga
      841 cgagacgccg ggccggcggg gacagggact gcatgtggtg ggcggtgagc gcggccgcgt
      901 tcggcacgag accgtcgccg tcgtcccacc gccaggcgtc acggccgcgg accagcgacc
      961 cgtcggcgat cgcggcacgg acgagctctc ggatgacgag ggcgttgccg tgtgagcgct
     1021 ccaacacccg gcgggcgatg tcgttgcccg ccggttcacc gagcgccgcg tgcaggaggt
     1081 cgccgcacgc cgcgaggtcg agcggtgcga catcgagtcg ccgcagcacc ccgaggtgct
     1141 cgagctgggc gacggcctcc gacgacggcg cgccggtgcg tgaggtgagg aggagcttcg
     1201 aggcgcccag tcgggcgacg tggaggagga cgaccgccga accggcatcg aggtggccga
     1261 cgtcgtcgac ccagaccagg tcgggagcgt cgatcatcca gcggtcgagc acggcctgga
     1321 cgtggccggt ggggtcgatc gacgtcacgt cgagcacctc gggccggagg agcccgatgc
     1381 cgtcgaagcg ggcgagcgtg tcgggcccgc ggaccttcac caccgacgca ccggagacgg
     1441 cggcgagcgt ggtcgccacg tgggccgcga tcgccgactt gccgatgccc gccgggccct
     1501 cgagcaggac cgctgcgacg tcggggtcct gcagcaggcc ggtgacgtgg tcgaccgttt
     1561 cctcacgccc gacgaagggc cacccagtca tgggccgcag tgtacgcatc gctccggggt
     1621 cgccagaatg ggccggtgcc cgcccccgag tccccgcctc actccgcgct gctgcggtcc
     1681 ggtcggggtg ccggagtcgg cgacggggag ctcgatccgg cctgggggca gctcgtcggc
     1741 gccgtcgagc agtgggccgg tcgctgcggt ggaaccgtgc tgaccnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnncgcga gatcgccgag tacgtcgtgg aaggctggcg
     1921 gaaccgtgag atcgccgacc gtctctgcgt gtcggtgagg accgtcgagg gccacgtgtt
     1981 gcgcctgtgc cgcaagctcg gcgtcaccga ccgcagtcag gtcgaggccc ggcatctgga
     2041 cccagccggc ggtggcggcg ctgcgggtcc ctgacatctg acgcgacgag cacgctgcgg
     2101 gaccttcggc cctcgctgcc caccctcggt gggcggatga tcgcgaggag accgagcaag
     2161 gaggcccgat gtcgatgaca acgaaccatc cgccgcaccg gcggctaccg gtcggacgga
     2221 tcgtcctcgt cgtgttgggg tgcgtcttcg tgcccttggc gctcgcgctc ctgggcggtg
     2281 gaacggtact gacctgggcg taccagaccc agcgggacgc gtccggctac ttcacgagtc
     2341 cgtccgagcg cctcgaaacc ctcgactacg cgatcgtgtc cgagcgtgtc gacctcgacc
     2401 tcgacagtgg ggactacctc ggcgtcggtc aactcggcac ggtgaggatc cggttggaca
     2461 gcaccaacga gcgccaggtc ttcatcggaa tcggaccccg cagggccgtc aaggactacc
     2521 tccgtggcgt ggcccgggca gagatcgccg acgtctcgac tgatccgttc tcggtcgagt
     2581 accggcatct cggcgacggt gcgccgaccg gtcccccagg cgacgagagc ttctggcagg
     2641 cacaggccga gggccctgga ccccagaccc tgacctggcc cgtcgagtcc ggcgactggg
     2701 tcgtcgtcgt gatgaaccgg gacgcaacgg cgggcgtcgg ggtcgacgcc gccgtcggat
     2761 tcaaggcgga ctggatccgg cccctcggta tcggcctgct ggtggccggc ggactggcgc
     2821 tgatcatcgc tgtcgccgcg ctcgtcgtcg gcatcgtcgg tctgcgccat cacgccgcga
     2881 gcgacgagac cgggggaacg catgtgcaca ccgggtccgg gccggttcgg ctcacgggtc
     2941 gactcgatgc ggagctgagc cggtggctgt ggctcgtgaa gtggttcctc gtcatccctc
     3001 acctcgtgat cctggccttc ctgtggttgg cgttcgcggt gctcacggtc gtggcgttct
     3061 tcgcgatcct cctgaccggc cgctacccgc gaggcatctt cgacttcaac gccggtgtgc
     3121 tgcgttggtc gtggcgggtg agctactacg cattcggcgt cgccggcacc gaccgctatc
     3181 cgccgttcag cctcggcgag catcccgact acccagctcg gctcgacatc gccccgccgg
     3241 accagctgtc gagaggactc gtcctcgtga agtggtggct actggccatc ccgcactacc
     3301 tgatcgtcgg tctcatggtc ggcacgacgg tgtcgagccg gagcggcgac cggtgggtcg
     3361 atgttccggt caccgggctg atctcctggc tcgtcctggt ggcggtcgtg acgctcctct
     3421 tcaccggccg gtacccgtcg ggcattcacc ggctcgtcgt cggcctccat cgttgggtgt
     3481 tccgcgtcgt cgcctacgcc gcgctcatga ccgacgagta cccgccgttc cgcctggacc
     3541 agggcgacga ggaaccgagc gtggactccg agctcggagc acctccgtcg tgaggcgctg
     3601 cagcgtcgag cgcgtcgcga cggcgggtgc gattgcggtg ctggccgccg tcgggcgtcg
     3661 gcgccacctg acctgggggt cgacgcccga cgaggtcgcc agggcactgc cgggtgacga
     3721 gctcgtgcgg tcgccgcacc tgacgtcgac tcgttcgatc acggtgcgcg ccgcagccgg
     3781 ggacgtctgg ccgtggttgg cgcagatggg ccaggggcgc ggcgggttgt actcctacga
     3841 ctggttggag aacctcgccg gttgccagat gcacagcgcc catcgggtgg tgcccgagtg
     3901 gcagtcggtg gaggtgggcg acccgtttcg cctccatccc gatctcgccc tgcgcgtggc
     3961 caggatcgat cccggcgagt cgctggtcgt cgagggggga gtgagcccca ccggacagga
     4021 gaccagcggt tccgacgccc cgtacgactt cagctgggcg ttcgttctcg tgccggtcgg
     4081 cgcggacgcc acccgactga tcatccgcga gcgctaccgg tatcacgcgg cctgggtgcg
     4141 gttgatggtc gagcccatct cggtgatcag ctgcgtgatg accgagcgga tgatgcgggg
     4201 gatccgcgac cgagccgagg ggacacgtag cggctcgtag cagccggtca tccggcgcgt
     4261 ggaacgaatg ccccgaccac ggctcagcgc gagcggagca cgcagaactc gttgccctcc
     4321 gggtcggcca acacgaccca cgacggtgtg ccctggccga tgtcgacgtg ggtcgcgccg
     4381 agggcgacga gtcgttcgac ctcggcgtca cggtcgtcgg gccggacgtc gaggtggaac
     4441 cggttcttca cggtcttcgg gtcgggcacg tgctggaaga ggacgccggg gaagcggtcg
     4501 gggcccgacc ggatctccac gtcgccgtcc tggtcgacga tgatctcggt ccatccgagc
     4561 gcatcggccc accagcgggc cagcgcgtcg gggtcgtcgg catcgatcac gagctgttcc
     4621 caggcgagtg tcatggtcac tggctaccaa atgagcgcgg gctcgcgcat ggctgtcggg
     4681 gccgcgatgg cgtcccgttc gaactgcgct gggtgatcct tcccgatcgg ccgaatccgg
     4741 accctcattg tcacacccat gtgcttgact gttcccatgg aggcgaacac cagttcgata
     4801 caggcggaga tggccgaggt gctcgcaccg ctgcggtccg tcctggctcg gctggcaccg
     4861 aacgacatcc cgctgcccga ggtggccggt atctggcagc tgttctccca ggtgcggcgt
     4921 cttgccggca atggcgagac gctgttggcc ggccgtgtcg ccgaggcgcg ggagtgggcg
     4981 cggtcggggt acgcgactcc tgaggaatgg atggcggcca aggccggcac gtcggtgggt
     5041 gaggcgcgcg gccagatcgc aacgtcggag cagctggggc aaggtctgga cgagacggcc
     5101 gagcgcatgc ggcaggggcg tctctcgccg cagcaggcgg gcatcgtggc cgacgcggct
     5161 gcggtcaatc ccgatgccga gctcgagctg tgtgaccggg ccgagcgtga cagcaacaag
     5221 aacctcagcg acgaggcccg tcggcgcaag gcgcaacgat ccgacggcga cgatcgggct
     5281 cgtcggcacc accgtgagcg tcgagcgcgg cgctactccg acgccgacga caagtcgcac
     5341 ctgcacgtcg agggacccac gtcgaagctc gcggagttct tcaaagccct cgatcgcgag
     5401 accgaccggc gataccgcga ggcgaccaag caggggcggc gcgagggccg cgacgcctac
     5461 gcgttcgatg ccttcctctc gctcgtcacc cagggaatcg ccggcgatgc cgcgacgtcc
     5521 tcgaagccga agcacctggc gatgatccga gtcgacctcg atgccctcgt gcggggccat
     5581 gtcgaggggg acgagacgtg cgagatcgcc gggttcggtg cgatctcggt cgctgaggcc
     5641 cgggcgttgt tgagcgagtc gatcctgtac ctcgtgtgca cgaagggcgt cgacgtcgtc
     5701 aacctcacgc acctcggtcg aggcccgaac cgagtgcagg agcttgccct cctgtgggcg
     5761 tcgcagacgt gccaggtcgc ggggtgctac cgaacccaca cgcaatacga ccatcggatc
     5821 gatttcgccg agacccggca cacccgggtc gacgagaccg acctgctctg cgtccatcac
     5881 cacaagatga agacgttcaa gggctacgcc ctcgtcgccg gcacgggcaa gcgacgcttc
     5941 gtcccgccgg gtgaccccga ccatccggcg acgtcgcgtt ctggtcccgg ctcacctcgg
     6001 tcggacgggt cgtctccggg gccagcgcaa cccccgccgc cggaaccgtc gcaggaaccg
     6061 ccgccgccct cggagtcgat cgaggagcgg atcggccggc tccgagcgag cgcccaggcc
     6121 cgtcgacgac gggccgccgg gcagggcgac ctgttcgggc tggcggagta gccgggtccc
     6181 ggactctgac cggacccggg cctcgacccg aatccggcgc aacgacggga gcaacccact
     6241 tcggtgggtc gcttcgtcgc gtcccgatgc ccgctcggag cggggccgtc cccggtacca
     6301 tcgaccgggg cggacagagc tgtccgccac caaggggggt tcatcgacat gtccactgac
     6361 gagcagatcc tcggccgcac cgaggtcgac gacctcgagg cgatcatctc gatgtacaac
     6421 accgacgtca ccgaggcgct gcacgtcgtc gcggacaacg ccccggcgat cttcacgtgg
     6481 gactacgaga agggcgcccg cccgcagctc aacaagctct acgagaaggc gaagcacgcc
     6541 cagtggaacg gcgagaccga cctcgactgg tcgatcgagg tcgaccagga gtacttcgcc
     6601 aagctcctct cggacatgcg ggccatgcag atggccgagg ccggcatcac cttcgacggc
     6661 accccgttcg ccaactgggg tgacaaggag tggctggccc actcgatgga gagccagaac
     6721 tggagtctca gccagttcat gcacggtgag cagggtgcgc tgatctgcac ggcgaagatc
     6781 gtggagacgg tgccgtggat cgacgccaag tactacgcgg cgacccaggt gatggacgag
     6841 gcccgccacg tcgaggtgtt cgccaagtac ctcgacacca agctcaacgg gcactacccg
     6901 atcaacacgc acctgcggat gctgctcgac gacatcatca acgactcccg ttgggacatg
     6961 acgtacctcg gcatgcagat catggtggaa gggctcgcgc tcgccgcctt cggcatggcg
     7021 atgcagacga cccccgaccc cctcctcaag cagctgctcc gttacgtgat gagtgacgag
     7081 gcccgccacg tcgccttcgg tgtgctgtca ctcaaggagt tctacgacgg gctgaccgac
     7141 gccgagctgt tggaacgcca ggagttcgcg ttcgaggccg ccgtccgcat gcgcgaccgc
     7201 ttcctccaac aggaggtgtg ggaccgcctc ggggtcaacg tcaaggacgc ggtgaagctc
     7261 ttcgacgtgc cggccgaggt caagaaccgc gacccgttcc agcagctcct gttctcgaag
     7321 atcgtgccca actgcaagaa gctcggcctg ctcgacgcca acgacggctg gctccgcaaa
     7381 cgtttcgaca cgctcggcgt cacccagttc gaggactggg ccgacaccgg cgaggagtac
     7441 ctcgagatgg acgccgtctc ccaggaccgc ctcgccgccc aggccgccgc cggctgacgc
     7501 ccaggccggg ggagcggggc gccgcgcgaa cggttcacag ggaattctgc gcccgcaccc
     7561 acctcccgcc cagcatcgcg accgatactg ctgagcgtga cgacgctgga gctcgatcgg
     7621 acgaccctcg atctcgaccc gaccgctccg acagcgggcc tgcgacgcca tgtgccccga
     7681 ttggtccggt acggcacggt gtcgatcgtg tcgacggcgt tcagtctgac gatcctgacc
     7741 gtcctcgtcg cgtccggcgc cctctccgcc ggttggggca acctcgtggc caccctgtgc
     7801 ggcgccgtgc cgtccttcga gctcaaccgc cggtgggtct ggcccggcgg tggccggccc
     7861 cgcctcgccc aggccgttcc gttcgtggcg atgtcgctcg ccggactggc gctctccgcc
     7921 gtcctcgttg ccgtgaccga gggctggtcg tcggaatggt ccgacggctc ccgagccctc
     7981 ctcgccgacg gctccaacat ggcggcgttc gggtcgctgt ggctcgtgca gttcgtcatc
     8041 tgcgaccgcc acctcttcgg gcgtcgctga ccgtcgggcc cacacccgcc gaggggcgag
     8101 tccggccaac gctccctgcc cggtagcgtg ccgctccatg gcccacgaac gcgaaccgtg
     8161 gttccacgac gagcacggca acctggtggt cgtccaagcg ccgaacctcc cgatcctggt
     8221 gtggctcggc gcggtggtgc tgcgcaccct cgtgcacggc ggggcacccg aacgcctcgc
     8281 cgacgccgtc gggttcggcg cactcttcac gtgggcctac ctcgaggtgg cgcagggcag
     8341 cgcccgcatc cggcggatcc tcggcgccgt ggtgctcgtc gccatggtcg tcggacgcgt
     8401 ccggggctga gcgcccggcc gtcgacgccg atccggcccg actacggacc gacgacgtcg
     8461 acgtgcccga gcgatgcggc ggtcacctcg gaatcgggtg agaggatcac gacctcggcc
     8521 cacgcgtccg cgcccagcac ccgggtgtgg gtgcagttgc ggacgacgat ctcctgatcg
     8581 ggcagcccgt cgacggcgac cttctctgcc gtcgtcgccg gcgtcggcac gctgccgtcg
     8641 gcaggtgcga gccgcggcag caactcggcg agcaggacgt ccttcgacgt ggtccggccg
     8701 ggcagcgggc acgtctcgcc gcggtcctcg acgagggcga tcgtgtcggt catcgtcggt
     8761 ggggccaacg acacgagccg gagcccgagg tccgacaccg ccgccacggc cggtggcgcg
     8821 gcgccgtcga agaacccgta gcgcgccgtc cccgcggtga ccacgagcag cgatcgacca
     8881 cacggcgtga gggtggctgc gatggtggga tcgagggacg tcactgcccg aacgaagtcg
     8941 tcggtggcgt cgcggaggtc ggcggggccg gtggacgagt cggcagcaat ccgttcgacg
     9001 gcggcgacga gatcggcatg gggcaacgcg gcagcgtcgg aggcgcaggt gatgccgtgg
     9061 cggaggagcg ccgtgatcac gtcggcctcg gccgccgagc cgtcgtcatc gccggcggac
     9121 accaccgccc ccggccggtc gaactcgccg gcgcccccga cggtgtcggc gaacttccag
     9181 acgccgaacg ctgctcctgc cacgacgccg atcgcgagca cgccgaagag ggcgatacgc
     9241 cacacggggc gacgtcgccc ctgccgacgg cgtgcggtgg aggtcggcgc catcgaccgc
     9301 ggcgtcggcg gtggtgcggc cgtgtcctcc caggccgcga ccgggggacc gaacgtcgac
     9361 ggtgccgggg gcggcgacgg tgtggaggcc gggtcgaccc cgaacggcgg catcgggggc
     9421 agcaccgagg tggacggcat cgggcgcacc tcgtcggtcc acgccgcgcc gtcccagtag
     9481 cggaggaggc cgggatcgga cgggtcggga aaccagccgg caacggacat gcggtgcgtg
     9541 agcctctcgg gtcgggggga acctcggtgg atcgacgggg agcgtagttc gtgctcgccc
     9601 cagcgcccgg gtgtggccgg gcgctggccc gtggtgttca ggccgggaac tcggctccga
     9661 gatcggccgc cttcttggcg gcctcctcgg ggaacacgtt cttcacgatg aacgggtcgg
     9721 cggtgagcga gtagtcgggg gcgaggtgct tggtgtagcg gtcgatgtag agcagctgct
     9781 tcgcgatcag taccagctcg cggggaatcg acgcgtcgta ggccttcagc atcttcaccg
     9841 actccgacac gagctgggcg atgtcgacgc cgccgaagcc gctcgacaac atggcgccca
     9901 gcacgttcgt gaggaacgcc gcgagctggt cctcgtcgcc gaactcctcg ggaatcgcgc
     9961 ccacccggcg gtacgcactg gccacccggg agaagtcgag gtcgaaggcg cacgtgtaga
    10021 acaggtcctg caacaggtcc cgccactcct ggtcgagctc gcccatgatg ccgaagtcca
    10081 agaagcaacc acggccgtcg tcgagcaccc agatgttgcc cgcgtgcatg tcgccgtgga
    10141 acgggccgtg cagcatggcc gcctcggccc acaccttggc gccgcgtcgg acgacgagct
    10201 gcaggtcgag cccgcggttc gccagttcgt cgaagtgatc catcgggaca ccccgcactc
    10261 gttccatgca gatcgtgcgg ggcccacagt ggtcccagta cacctcgggc gccgtgatca
    10321 tcgtgttgtc gccgaacgcc ccgatgcggt cccggaagcg gtcctgctgc cacgcctccc
    10381 ggacggggtt gagttcctgg aacgtgacgg cgtgaaggtc gacgatgatg tcccgtgctc
    10441 cggaggcctt cccccacggc gtcttctcga acagccaggc gatgcggtac gagttgcgga
    10501 gatcctgggc catgccgtcg cggatgccgg gtcgctgcac cctgcgtgac ggcctcgcgg
    10561 ccgtcacgca gggtgcaggc gtggacctgc ccgatcgacg cggccgacaa cgggtggtcg
    10621 tcgaacgagg cgaacacctc ggtgacggat tgccccaggt cctcggcgac ggcggcccgt
    10681 gcctgttcgc cggggaacgc cggtacggcg tcgaggcaac ggcgggcggc gaggaccagt
    10741 ggctcgggga ccagtgcgcc cgagctggcg aggatctgcc ccgccttcac gaaggtcggg
    10801 cccagccgga tgaacccgtc gacgaggccg tcgcaggccg tcgcgacgat cggcgtggag
    10861 cggcgtcgca ccaggcgacc ggcgagtgct cggaggaccg acgtgcccag gacggcgacg
    10921 acaacgacga atcgcccgac gtcgcggacg tggaacgtgt cgagcggcgg cgcgtcgacc
    10981 tcgagcgcgg cggcgggcgg cccgtcggcg tacggacccc ggaagggggt ggccgagcgt
    11041 cgaccggtgg tctcgccggg gctcgtggtc atccggatct cctcgccgtt gttgacctga
    11101 cgtcaatacg agggacacta ggcccggccg tcgggcccgg tcgagaccgc gctgggtcgt
    11161 tcgggccaag cagccgggag aaccacccac ggtccggcgg cggtcatcac tcaagacgga
    11221 tccgagggac cccgaaggtc tcttcatggc aggacgaacc gcggagcgcg ccgcccggag
    11281 ggcccggcgg cgcttccgcc gtctctacag ggtcgctgtt gtcctgatcg tcgcgttcgg
    11341 cgtcatcctc gtccgcaacc ggggtgacga cccggtgtgg accgccgtcg acactgccgg
    11401 ccagctgctc gcggccgtcc tcgctgccgc cgccggagcc gtgaccgccc gccgcagttc
    11461 cggtcgagca cggtggggat ggagcaccat cgccgccggc tgtgcggcgt gggctgtcgg
    11521 gcggggcatc tggctcgtgt ccgcgatggt gctgcaccac aacgtgccgg caccgggcat
    11581 cgccgacatc ggtttcgtcg ccgcgcccgt cctgtggacc gccggcatcc tgatgctcgt
    11641 cgacacgccg cccggcacgc gttctcgcac gagcggtctc ctcgagggcc tgatgatcgc
    11701 cggtggagtg ttcctcccca cctgggcact ggcattggct cccgtcgtgc gggagtcggg
    11761 cgggccgggc ccggcgcaga tcgtggacct cgcgcgcccg gtgctcgacc tcgtggtgct
    11821 cgcctcgctg ctgttcgcgt tcccccgctg ccgccgccac gctcggggaa gcctcgcgct
    11881 cctggcgatc ggcgtggccg cgatcgcgac cgccgacagc gggttctggt acctccgcac
    11941 cgtcgactcc tacatcggga cgaacctgtt cgatctcggg tggttcgccg ggttcctgtt
    12001 catcggactc ggatcgttgc gaccttcggc cggaacgccg acgggaacgt cggctggcac
    12061 ccaggccggg gcgccggctc tcgacccgga gttccgggac agctggagac tcctcagggc
    12121 tgccgcccgg acgttaccga cggtcacaag ccgtggatga ggcggaatgg cattgctgag
    12181 gtcgccgctc gggcgtcctc aatgctccca gcgtcgggga tagcaccact gatctcgatc
    12241 gaagggatcc gtctgaaacg cgcacatgtg gggcagacgc gaccggtccg tccccgcccg
    12301 gtggtcgctg tttcgggact ggccttgatg aggcctttgc ccgaacactc gtggcacggc
    12361 atcgtcccga ctacccggac tcggatgtcg aggagctgga tgatgttgag ccgaaggtca
    12421 tcggcggcgt ggtccagcgc gtcgcgagcg gtgtccgcga gggtgaggag tcgtctggcg
    12481 tggtcgctct gagtgtcccg ggcggcggcc cagcccacga ctcgatctcg gtgcatcttc
    12541 aatcgctcga gttcctcttc gagctgccgt gtcgcgtgcc ggatcgccac ggggtcagtt
    12601 cctgaggtga cgagttgagc gatgcttgat ccaagggccg actcgatgcg gcggatcttt
    12661 cgggtgagcg cggtgaggtc gtctgcccct gctggttggt gcgcggagtc ggcgatgcca
    12721 gcgagcgtca ggagccgctc ggggtcggtg agcatctcgt gggtggcgga ccagactcgg
    12781 gcctccacgc cggctgcgtg aatccgggga caatcgcatc ttgcggggcc acgctcggcg
    12841 aggttgttgg cgcatcggta gacgcgagcg tcgcggtcgg ggcgtcccgt gccctgcata
    12901 tttgacccgc agggggaggt gagtcgcccg ccgaggaggt atctgtgccg ggccagagtg
    12961 gctccagggc cggtgctggt ctcagcgagg cgttggcgga gctgttcgtg ccttggttcg
    13021 ctgatgatgg ccgggatcga gagcgttatg gtgtgggtgt cggttgtgta tgtccaggtc
    13081 ccggagatcc ccgttgcggt tctgagcatg tcgcgcactc gtcggtgcgt ccaccggccc
    13141 gtgccggggt tggagaggcg acgggtcgct ccgggtgttg ggacgtcccg gtcgttgagg
    13201 tcgtcggcga tcttgagcgt tgatcggcga tcgtcgatga actggtcgac caccgatgcg
    13261 atgacctcca cctcttgagg attcagcgcg aggcgactgt gggcttcacc ctcgtcgcgt
    13321 acaactgtgt agccgaaggg cggtgggccg ccgggccagt agccagcttc ggcgacggct
    13381 cggattccac ggaccatgcg gtcacgaatc ttccgtcgct cctcctcagc gatcactcca
    13441 cgaagttgtc gctccagagt gctcatcgtt ggctgaacga gatagacgac accgacctca
    13501 gcgcgagcaa gttcgcgctc gaggaccaga ccatcgacca ggtcacgaga gagccgatcg
    13561 gggtccgtca caaccacgac gtcgaaggaa ccctctctcg ccgactggag gaggccttgg
    13621 agtgcaggcc gatgaatcgt tgctccagac acgccgtcgt cgacgaactg ccccaccacg
    13681 ttccagccgc tcgtctcgca gtaggccgca gcgcgtcgtt gctggtcggc gaggcttgtt
    13741 ccgtgcgtcg cctgctcgtc ggtcgagacg cggctataca gcgcggcgcg ggtggttcgg
    13801 ctcattgggg gcctcgagct gatcagttga cctggaggcg gtgagaagta ccgcgagctg
    13861 cgccatcacc ggcaacgagg ccggtcgcca ctcgcaggac gctaacaccg gcgagtagag
    13921 cgaatcgtcg aatgctggtc tcggatcgag tggatcgtcc atcagtagcc ctaagcgcgt
    13981 gtaaagcgcc gcggtcgtga gaattctgtg caatgacgat tgacgctgca gagcctgttt
    14041 cggaaacctc gcagctgctt cgccagcagg gctccggttg ctgtt
//
LOCUS       DAOSOA010000045        13994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4383_length_13994_cov_7.408996, whole genome shotgun sequence.
ACCESSION   DAOSOA010000045 DAOSOA010000000
VERSION     DAOSOA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13994
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4383_length_13994_cov_7.408996"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKY13_04770"
     CDS             complement(<1..742)
                     /locus_tag="PKY13_04770"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOT79273.1"
                     /translation="MTSTDRDDQTDLAARTDLAGLVADYERDGAVVVRGALSAEQVTL
                     ATAGIEHVLAHPSPLALTASAADDPGRFVEDFRTWQQVPEIETLARDAGLARLAAALM
                     GSATARFFHDHVLVKEAGTRQRTPWHQDLPYFNIDGSQNVSFWIPVDPVPRAGSLEFV
                     AGSHLGPWCTPRTFMDHVAKWFPDGALAELPDIEADRDAFPILAWDLEPGDLVAFHML
                     TIHGAAGFAGPGRRRVLSLRYTGDDAVHA"
     gene            complement(739..1299)
                     /locus_tag="PKY13_04775"
     CDS             complement(739..1299)
                     /locus_tag="PKY13_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT79274.1"
                     /translation="MKPALNQRDAQKDATRRALSGAAIDLFTTKGFDETRVEDIAAAA
                     GVTSRTFFLHFPTKEAAAFPDHNERVDALADALATDDSPDPLPLLRELIVLGVRATSG
                     SRIRAKRYRLFNEIEALRHRDVLGDLDYERVIVDHLMSRSVDEFHARVLAVSIMGIAR
                     SALTTWAHDPSCDPGNAAEAALEQLR"
     gene            1357..2751
                     /locus_tag="PKY13_04780"
     CDS             1357..2751
                     /locus_tag="PKY13_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOT79275.1"
                     /translation="MSAYPYQDRFPVNRTLPEHGRPRDEIIEEMRMLAEEEDRSWESG
                     RVSGTMYCGDHDHYSFMNDVFGLYAHVNILQRDICPSATRYEGEVIAMALDLFHADAV
                     TDGEPVGLITSGGTGSICHAVLAYREKAVAERGVTRPNFIKPETGHPAFDKACHLFGI
                     ELRKAPVDPETTLVDVDWVRANIDDQTIGFMGSACNYGYGTIDPIEQLSDLAVETGVP
                     LHVDGCLGGFILPFGQELGFPNIPLFDFRLPGVTSISADTHKYGYAFKGTSTVLFRDK
                     SWRNSQYFFLTDWTGGKYTSPGMDGSRSGGLLAATWASMVQLGREGYRKYAQAIFETS
                     AAMQDAVRSHPELRILGAPSFLFSFTSDEFDVYHINDFMRPKGWRFNGQQYPNALHMA
                     VTRPQTQEGVVEAFVADLAEAVVYAKEKGHEPASSGAIYGGVAGGMNDEADEFIKMVM
                     ASMLDEQQALPPLS"
     gene            2782..4347
                     /locus_tag="PKY13_04785"
     CDS             2782..4347
                     /locus_tag="PKY13_04785"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT79276.1"
                     /translation="MSADVSDPRDDRDSQFVLVVDLGTGGPKVGFASLLGRVVHQEHH
                     EVETTFTADGGATQDAEEWWRLIIEGAKRALDSGAVDPAKVVAVSMTGQWASTVPVDE
                     AGIPTGPVVMWMDSRGGELVRQKMGGAVSGYKARGIAEFIRRSGGAPSLDGADPIGHR
                     LFLQHHDRELWDRSRWLLEPVDYLSMRFTGKPAASPVSMTGSWLVDTRDPSNISYDPV
                     LVELAGGGVEKLAPLQKFGTVMGVVQPSVAADLGFGPDVKVVTGAPDLHTAAIGSGAV
                     RLGEAHMAVSTTGWISVPITRKKTDIVRNCATVPGIDDATYLVANNHETAGMCLQWLK
                     GAMGGTLDYEQLTELAGTADAGSGGVIFTPWLKGERSPVADRTMRAGFHNMSLATTQG
                     DLIRSVLEGVAYNNQWLHEAVEKFVGGRLDNIRFIGGGAQSDLWCQIHADVMDRTIEQ
                     VTDPLYCGLRGAALSAAMTLGEVRQEELRGLVTVKQTYVPNPANRAAYDRLYAEFPGL
                     YSSQKKFFRRLNR"
     gene            4360..4644
                     /locus_tag="PKY13_04790"
     CDS             4360..4644
                     /locus_tag="PKY13_04790"
                     /inference="COORDINATES: protein motif:HMM:NF016162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HOT79277.1"
                     /translation="MATATWNGKVIASSEDTVVVEGNHYFPIDSVTDGVLTPTDTQSV
                     CPWKGTASYYSVVVDGDTNTDAAWCYPEPKDAAKEITGRVAFWKGVEVTS"
     gene            4727..6727
                     /locus_tag="PKY13_04795"
     CDS             4727..6727
                     /locus_tag="PKY13_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOT79278.1"
                     /translation="MSGQGPLRKFVGQDKGRAKFSVTVPRPEPLPHVDRLLAGAARVD
                     ITPPPGMPKAGYSSNAHDGCGFRSRLRAHVLHLRCGTSSIALVQCDLLGGSSVVQHLV
                     ADAIRERTDIALAGLMMGATHTHAGPGQFSGTDFYNRFASNRGGFDPAWTQFLVDRIA
                     GAVIEAHDTRQPARLAFGSTEVWGSTRNRSLDPHVQNDTVLDKRLDPQRKFANINPEL
                     QLLRVDSDPETGTGSAPIGTAVIFSVHGTGISQHAHEYNADLWAYLVDELGHRIETSR
                     GTRGVVGAMEGTHADMAPALHPGRAGHLEARRLGRQIGGEAADLYADLADELTATPDL
                     ACGFREVNLEGGASIDGIRLPDRPAVGAALVAGAMENLTPVIHAIPPFKAGFPKPFDV
                     TGPQAQKWVLGSKWLQPLILPLKGFPRLLPVHVLQIGATAIVGLPFEVTVESGRRIAA
                     AANSALPPDSSIGRVVVSSVANEYFGYVATAEEYSRQYYEGGHTLYGPNTQRFVAAHV
                     AKLVGEVATVDAVLPVISDVAPQRSWDLKVHRYLCRPSARGVGRAAVLAPTYVDATGD
                     DDGYWEFRWRDAPPGDLVWHEPLVRIEARDGDGEWHAATHRGRRADDQGTDVEVRLVG
                     SDAIGCVYAARWHDPAFKAGRTHRFVVLGSGSATELTSAPFD"
     gene            6735..7637
                     /locus_tag="PKY13_04800"
     CDS             6735..7637
                     /locus_tag="PKY13_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT79279.1"
                     /translation="MAATPTGSAAKGMTLRGFSLVMISIVSVQFGAAIAKEIIDIAGP
                     IGTVFYRVAFAALLLCAFVRPQVRGRAREDWGYIAAFGLVLAGMNVCYYAALQHLPIG
                     VTVTIEFTGPLAVAVLGSRRRIDIFWALLAAVGLVLLSPVTGVDLPIVGVLLALAAGA
                     FWAGYILLSSRVGERVSGLSGLALALVASAAVTAIPAVATAGTTLLDPKVIGIGLVVA
                     LMASAIPYGCELEALRSLTTASFGMLMSLEPAFAALAGAVVLSERLGAPELLAIGFVL
                     VANAGAVLTRRARTPDVDVGIVQP"
     gene            7634..8353
                     /locus_tag="PKY13_04805"
     CDS             7634..8353
                     /locus_tag="PKY13_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79280.1"
                     /translation="MTGIPTERWTEFDTPDGDHWRVDLGFLASGWTCIWGAGCRGIGP
                     DADPTHIMGCCSLGAEFADPDDARNVAAHAAFLDPERFQFAAVAEADGIFADESRRAT
                     RVVDGGCIFLNRPGFEGGAGCALHQAALADDEPPRDWKPVVCWQLPFRIDVESGVTIV
                     RRWARADFDEPDDPSASIPWLCTEDEAAYLADASALDRHRDDLVELLGEAAVAEIGAR
                     CSVDAEAGDDVGVEFDTEPDA"
     gene            complement(8291..9379)
                     /gene="menC"
                     /locus_tag="PKY13_04810"
     CDS             complement(8291..9379)
                     /gene="menC"
                     /locus_tag="PKY13_04810"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOT79281.1"
                     /translation="MRIESIEVIAVRLPLRSPWHTAAGTLNGREVLLVRLRSDIGDGW
                     GECAAFAEPGYSAEFVASARIVLRDHLIPRLLAPTDIAASEAATIMSPVNGHRMAKSA
                     LEMAMLDAELRAAGTSFGDFLGVTRTHVSAGVAIGFAGSPAELCDRVAAYVDEGYRRA
                     KLKIAPGRDLDYIASVRDQFPALALQVDANGAYDRDDLDHLAKLDEFGLLLVEQPFGE
                     DDLVGHARLAERLVTPVCLDEPIVSIDTTRTAVALGACDVVNLKPGRVGGYLEARRIH
                     DWCVQQSIPLWCGGMLETGLGRAANVALAAMAGFTLPGDLSASARFYGTDIVTEPIVV
                     EHGSIAVPSGVGFGVELDPDVIARFRVD"
     gene            9461..10243
                     /locus_tag="PKY13_04815"
     CDS             9461..10243
                     /locus_tag="PKY13_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT79282.1"
                     /translation="MLTITDQDRVRLLTLDRPDALNAFNEALYDAVSEALIEAAGDDA
                     VATVVITGSGRAFSAGQDLLEMSERITNPDFVQGTHGFPGLIDQLAAFPKPLLLAING
                     LGLGIGTTIIGFADLVFMSTDARLKCPFTSLGVAPEAASSLLFPRLLGHQNAAWVLLS
                     AEWISAEEAKELGLAWKVTSPEELLDVTMAHATKLAAMPISSLIAVKGTMTAGRVEAT
                     RAARDLEDAQFQKLMGGPANMEALAAFAERRPADFTNLPPGW"
     gene            complement(10218..11609)
                     /locus_tag="PKY13_04820"
     CDS             complement(10218..11609)
                     /locus_tag="PKY13_04820"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT79283.1"
                     /translation="MNAPVDDAASTRPVVPSLKEVATQRSVVIFLLATFILEIGISSQ
                     FTALGKRVFDIGGRELDLGLLGLAEFLPVALLVLLTGAVADRFDRRKVASIALVGEAA
                     FTLLLIVEVERSDATIGPIFGLVVGYAIARAFLSPAIRALPSTIVAPEALPRISPLYS
                     GAFQAGIIAGPVIGAFLYTIDVWVPFAVSAGCLLVGAVLVLFVQLHPTYEHRAAESAR
                     RDERASGTSGTSKVSEALEGLKVIRKEPLLLGAISLDLFAVLFGGAVALLPAVAEQRL
                     GVGVVGLGWLRAAAGLGAGLMTATLAFRPVQRHVGATLFATVGVFGAATIVFGFTRNF
                     GVAFGALVVLSGADSISVFIRSTLVPLVTPNEVRGRVGAVENVFIGASNELGAFESGL
                     LGQALGAGPAIVAGGAATIAVVLIWPMLFPAIRRLDHFPFHHSFAASTGTAVPAAADE
                     AVLLPTTNREADS"
     gene            complement(11606..12085)
                     /locus_tag="PKY13_04825"
     CDS             complement(11606..12085)
                     /locus_tag="PKY13_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79284.1"
                     /translation="MALTRPTPTVIAHDPSALAAVMAELVEARSGWINLLPEIPEDVP
                     VPSTPSALAVFSKRGPVVPLGTWTAPTPGRNGTTAPVEIGLQHGAAKQARAALADTPG
                     SIPPEWAVLSDNPRRGLVVRPPAGTSLVAEATWLLAALTAICIPPQTGGFVMFRYGQ"
     gene            12110..12955
                     /locus_tag="PKY13_04830"
     CDS             12110..12955
                     /locus_tag="PKY13_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT79285.1"
                     /translation="MTTGFAVSPEHVVNDPADARIGEFVGLRDRDLRVRDRATAATDS
                     PLDAVFIAEGDPVVARAVRAGFDLVTLLVDATRTRPLPDGVEAAAGVIYGAGPEVLQR
                     ITGMGVHRGCLGVFRRRPLPSLDEVLTGARRVLVMERVVNPINLGVIARTAVGLGMDA
                     MVLDDASVDPLYRRASRVAMGEVFEFPYARAGRFPAGLEAVGAAGFTLVALTPADGAE
                     SIDDIAFGPDERVALVLGSEGPGLTDETMERCDRRVSIPLSGNVDSLNVGAAAAIACY
                     AITRA"
     gene            12991..>13994
                     /locus_tag="PKY13_04835"
     CDS             12991..>13994
                     /locus_tag="PKY13_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT79286.1"
                     /translation="MSRPWWHTTSIYQVYPRSFADSNGDGIGDLPGIVGHLDHLVDLG
                     VGAIWVSPFFASPQRDIGYDISDYRSVAPEYGTLDDARTLIDEAHRRGLRVLFDLVLN
                     HTSDQHAWFRESRSSRTNPKADWYVWHDGRVGSGGRRLPPNNWRSELQLTKAWQWSDE
                     REQWYLATFLPFQPDLNWRHPDVRSEMFDTVRSWLAMGVDGFRLDIFGSIMQDDQLRD
                     NPRRPDLSFGLPRVQQPTNTLNTDDNFTLAGDLRAVCDEFTPPPGTPEIPGAGERILL
                     GEVFGPAETLRRYVRDGDRDGLHLVFLFDFLTATYSAAGYRRLIERFEAEFPDPLQPT
                     "
BASE COUNT         2128 a         4768 c         4941 g         2157 t
ORIGIN      
        1 gtgcgtgcac ggcgtcgtcg ccggtgtagc gcaacgacag cactcggcgt cggccggggc
       61 cggcgaaccc ggcggcgccg tggatcgtga gcatgtggaa cgcgacgaga tcgcccggct
      121 cgaggtccca ggcgaggatc gggaatgcgt cgcggtcggc ctcgatgtcg ggaagctcgg
      181 cgagtgcacc gtcggggaac cactttgcga cgtggtccat gaacgtgcgc ggtgtgcacc
      241 aggggccgag gtggctgcca gccacgaact cgagcgaccc ggcccggggt accgggtcga
      301 cggggatcca gaagctcacg ttctgtgagc cgtcgatgtt gaagtagggc aggtcctggt
      361 gccagggcgt gcgttggcgg gtccccgcct ccttcacgag cacgtggtcg tggaagaacc
      421 gtgctgtcgc cgaccccatg agcgcggccg cgagccgggc gaggccggcg tcgcgtgcga
      481 gcgtctcgat ctcgggtacc tgctgccagg tgcggaagtc ctcgacgaac cggcccggat
      541 cgtcggcggc gctggccgtg agagcgaggg gcgacggatg ggcgaggacg tgctcgatgc
      601 cggcggtggc gagggtgacc tgctcggcgc tcagcgcgcc gcggacgacc actgcgccgt
      661 cacgttcgta gtcggcgacc aggccggcca ggtcggtgcg ggcggccagg tcggtttggt
      721 catcgcggtc ggtggaggtc atcgcagctg ctccaatgcg gcctcggcag cgttgccggg
      781 atcacaggac gggtcgtggg cccaggtcgt cagggccgaa cgggcaatgc ccatgatgga
      841 cacggccagc acgcgggcgt ggaactcgtc gacggagcgg ctcatcagat ggtcgacgat
      901 gacacgttcg tagtcgaggt cgccgagcac gtcacggtgg cggagcgcct cgatctcgtt
      961 gaagagccgg tagcgcttgg cccggatgcg cgagccggag gtggccctga cgcccagcac
     1021 gatcagctcg cggaggagcg ggagcgggtc gggggagtcg tcggtggcga gcgcgtcggc
     1081 caacgcgtcg acgcgttcgt tgtggtcggg gaacgccgcc gcctccttgg tcgggaagtg
     1141 gaggaagaac gtgcgggacg tcacccccgc tgcggcggcg atgtcctcga cccgggtctc
     1201 gtcgaatccc ttggtcgtga agaggtcgat cgcggcaccg gacagcgctc tgcgggtcgc
     1261 gtccttctgt gcatcgcgtt ggttgagcgc cggtttcacg gcggagaccc tagaccagtt
     1321 gacttcactg agtgaagtga aatactctcg ccctcgatga gcgcctaccc gtaccaggat
     1381 cgtttccccg tcaaccgcac gctgcccgag cacggccgac cccgtgacga gatcatcgag
     1441 gagatgcgca tgctcgccga ggaggaggac cgctcctggg agagtggccg cgtctccggc
     1501 acgatgtact gcggcgacca cgaccactac tcgttcatga acgacgtgtt cggtctgtat
     1561 gcgcacgtca acatcctcca gcgcgacatc tgtcccagcg ctacccgcta cgagggcgag
     1621 gtcatcgcga tggcgctcga cctcttccac gccgacgcgg tcaccgacgg cgagccggtg
     1681 ggcctcatca cgagcggtgg caccggcagc atctgccacg ccgtgctcgc ctaccgcgag
     1741 aaggcggtcg ccgagcgcgg ggtgacccgc cccaacttca tcaagcccga gacgggacac
     1801 ccggcattcg acaaggcctg ccacctgttc ggcatcgagc tgcgcaaggc gcccgtcgac
     1861 cccgagacca cgctggtcga cgtcgactgg gtgcgggcca acatcgacga ccagaccatc
     1921 ggtttcatgg gctctgcctg caactacggc tacggcacca tcgatcccat cgagcagctg
     1981 tccgatctcg ccgtcgagac cggtgtcccg ctccacgtcg acgggtgcct cggtggtttc
     2041 atcctgccgt tcggccagga gctgggcttc cccaacatcc cgctgttcga cttccgcctg
     2101 ccgggcgtga cctccatctc cgccgacacc cacaagtacg ggtacgcctt caagggcacg
     2161 tccaccgtgc tgttccgcga caagtcgtgg cgcaactcgc agtacttctt cctcaccgac
     2221 tggaccggcg gcaagtacac gtcgccgggc atggacggct cccgctccgg tgggctgctc
     2281 gccgccacgt gggcgtcgat ggtgcagctc ggccgtgagg gctaccgcaa gtacgcccag
     2341 gcgatcttcg agacgtcggc ggcgatgcag gacgcggtcc gaagccaccc cgagctccgc
     2401 atcctgggcg ctccgtcgtt cctgttcagc ttcacgagcg acgagttcga cgtgtaccac
     2461 atcaacgact tcatgcggcc caagggttgg cgcttcaacg gccagcagta cccgaacgcg
     2521 ctgcacatgg ccgtcacccg tccgcagacg caggagggtg tggtcgaggc gttcgtcgcc
     2581 gacctcgccg aagccgtcgt ctacgccaag gagaagggcc acgagccggc atcctccggg
     2641 gccatctacg gcggcgtggc cggcggcatg aacgacgagg ccgacgagtt catcaagatg
     2701 gtgatggcca gcatgctcga cgagcagcag gctctgccgc cgctgtcctg aacaggagcg
     2761 ggtcgctagc ctgcgccctc gatgagcgca gacgtcagtg atccacgaga cgatcgagac
     2821 agccaattcg tcctggtcgt cgacctcggt acgggcggcc ccaaggtggg gttcgcctcg
     2881 ctcctcggcc gcgtggtgca ccaggagcac cacgaggtcg agacgacctt caccgccgac
     2941 ggcggcgcga cccaggacgc tgaggagtgg tggcgcctga tcatcgaggg agcgaagcgc
     3001 gccctcgact cgggcgccgt cgatccggcc aaggtggtcg ccgtttccat gaccggccag
     3061 tgggcctcga cggtgccggt cgacgaggcc ggcatcccga ccggaccggt cgtcatgtgg
     3121 atggacagtc gcggtggcga gctcgtccgc cagaagatgg gaggcgccgt gagcgggtac
     3181 aaggctcgtg gcatcgccga gttcatccgt cgctccggag gggcgccgtc gctcgacggc
     3241 gccgacccga tcgggcatcg gctgttcctc cagcatcacg accgtgagct gtgggaccgc
     3301 agccgttggc tgctcgagcc ggtcgactac ctgtcgatgc ggttcaccgg gaaacctgcg
     3361 gcatcgccgg tgtcgatgac gggctcgtgg ctggtcgaca cccgtgaccc gtcgaacatc
     3421 agctacgacc cggtgctcgt cgagctcgcc ggcggtggtg tcgagaagct ggcgcccctg
     3481 cagaagttcg ggacggtcat gggggtcgtg cagccgtcgg tggcggccga cctggggttc
     3541 gggcccgacg tcaaggtcgt gaccggcgcc cccgatctgc acacggcagc gatcgggtcc
     3601 ggcgccgttc ggctcggtga ggcgcacatg gccgtcagca cgaccggctg gatcagcgtg
     3661 ccgatcaccc gcaagaagac cgacatcgtg cgcaactgcg cgacggtgcc cggcatcgac
     3721 gacgccacgt acctcgtcgc caacaaccac gagacggcgg gcatgtgctt gcagtggttg
     3781 aagggagcca tgggtggcac gctcgactac gagcagctca ccgaactggc cggtaccgcc
     3841 gacgcgggct cgggcggtgt gatcttcact ccgtggctga agggcgagcg gtcgccggtg
     3901 gccgaccgca ccatgcgcgc tggcttccac aacatgtcgc tcgccaccac ccagggcgac
     3961 ctcatccgtt cggtcctcga aggggtggcc tacaacaacc agtggctgca cgaggcggtc
     4021 gagaagttcg tcggtggacg cctcgacaac atccggttca tcggcggcgg tgcgcagtcc
     4081 gatctctggt gccagatcca cgcggacgtg atggaccgca ccatcgagca ggtcaccgac
     4141 ccgctctact gcggtcttcg tggcgcggcg ctgagcgcgg cgatgacgct cggcgaggtc
     4201 cgccaagagg agcttcgggg gctggtgacc gtcaagcaga cgtacgtccc gaaccctgcc
     4261 aaccgggcgg cctacgaccg gctctacgct gagttccccg gtctgtacag ctcgcagaag
     4321 aagttcttcc ggcggctgaa ccggtgaagg agcgcagtcg tggcaaccgc aacctggaat
     4381 ggcaaggtca tcgcgtcgag cgaggacacc gtggtcgtcg agggcaatca ctacttcccg
     4441 atcgactcgg tgaccgacgg ggtactcacc ccgaccgaca cgcagtccgt gtgtccgtgg
     4501 aagggaacgg cgagctacta ctcggtggtc gtcgacggcg acaccaacac cgacgcggcc
     4561 tggtgctacc ccgagccgaa ggacgccgcc aaggagatca ccggtcgggt cgccttctgg
     4621 aagggcgtcg aggtcacctc ctgaccacgc cccctggggt tttgtactgc gaggagcgac
     4681 accagcgtcg ttcctcgcag tacaaaaccc cgagtggggg tctgtcgtga gtgggcaggg
     4741 gccgttgcgg aagttcgtcg ggcaggacaa gggccgggcc aagttctcgg tcacggtgcc
     4801 gcggcccgag ccgcttcccc acgtcgaccg gctgctcgcc ggcgccgctc gcgtcgacat
     4861 cacgccgccg ccgggcatgc cgaaggccgg ctattcgtcc aacgcccacg atgggtgcgg
     4921 gttccggagc cgtctccgcg cccacgtgct ccatctgcgc tgcgggacgt cgtcgatcgc
     4981 gctcgtgcag tgcgacctgc tcggcggctc gtcggtcgtg cagcacctcg tcgccgacgc
     5041 catcagggaa cgcaccgaca tcgcactcgc cggtctcatg atgggtgcga cgcacaccca
     5101 cgccggtccg ggccagttca gtggcaccga cttctacaac cggttcgcgt ccaaccgcgg
     5161 cgggttcgac ccggcgtgga cccagttcct cgtcgaccgc atcgccggcg cggtcatcga
     5221 agcgcacgac acgcggcagc cggctcgcct cgcgttcggt tcgaccgagg tctggggctc
     5281 cacccgcaac cggtcgctcg atccgcacgt gcagaacgac accgtgctcg acaagcggtt
     5341 ggatccgcag cggaagttcg ccaacatcaa cccggagctg cagctgctcc gggtcgacag
     5401 cgatccggag accggcaccg ggtcggctcc gatcggcacc gctgtcatct tcagcgtcca
     5461 cggcaccggc atctcccagc acgcccacga gtacaacgcc gatctgtggg cgtacctcgt
     5521 ggacgagctc ggtcaccgga tcgagacgag tcgcggcacc cgcggcgtcg tcggcgcgat
     5581 ggagggcacg cacgcggaca tggcaccggc gctgcacccc gggcgcgccg ggcacctcga
     5641 ggcccggcgg ctgggacgcc agatcggtgg cgaggcagcc gatctctacg ccgatctcgc
     5701 cgacgagctc accgcgaccc ccgacctcgc gtgcggtttc cgcgaggtga acctcgaggg
     5761 cggcgcatcg atcgacggca tccgcctccc ggaccgtccg gcggtgggcg cggcgctcgt
     5821 cgccggggcc atggagaacc tgacgccggt catccacgcc atcccaccgt tcaaggccgg
     5881 tttccccaag cccttcgacg tcaccggccc ccaggcccag aagtgggtac tcgggtcgaa
     5941 gtggctgcaa ccgctcatcc tgccgctcaa ggggtttccc cgcctgttgc cggtgcacgt
     6001 gttgcagatc ggggcgacgg cgatcgtggg tctgccgttc gaggtcaccg tcgagtcggg
     6061 ccgtcgcatc gccgccgcgg ccaattccgc gctcccgccc gactcctcga tcggccgcgt
     6121 cgtcgtgtcg tcggtcgcca acgagtactt cggctacgtg gcgaccgccg aggagtactc
     6181 ccgtcagtac tacgagggtg gtcacacgct ctacggtccg aacacccagc gattcgtcgc
     6241 agcgcatgtg gcgaagctcg tcggcgaggt cgcgacggtc gatgccgtgc tccccgtcat
     6301 cagcgatgtc gcgccgcagc gcagctggga tctgaaggtg caccgctacc tctgccgccc
     6361 gtcggcccgt ggtgtcggac gcgccgccgt cctcgcgccg acctacgtgg acgcgaccgg
     6421 tgacgacgac gggtactggg agttccgctg gcgagacgcc ccgcccggcg atctcgtgtg
     6481 gcacgagccg ctcgtccgca tcgaggcgcg tgacggtgac ggcgagtggc acgcggcgac
     6541 ccatcgcggg cgtcgggccg acgaccaggg caccgacgtc gaggtgcgac tcgtcggcag
     6601 cgatgccatc ggttgtgtgt acgcggcgcg ttggcacgac ccggcattca aggcgggtcg
     6661 cacccatcga ttcgtggtgc tcggatcggg gtcggccacc gagctgacgt ccgccccgtt
     6721 cgactgacgc cgaggtggcg gccacaccca cgggaagcgc tgcgaagggc atgaccctcc
     6781 ggggcttctc gctcgtgatg atctcgatcg tgtccgtgca gttcggcgcg gccatcgcca
     6841 aggagatcat cgacatcgcc ggcccgatcg gcaccgtgtt ctaccgggtc gcgttcgctg
     6901 cgttgctcct ctgcgcgttc gttcgtccac aggtccgtgg ccgcgcccgc gaggactggg
     6961 ggtacatcgc cgcgttcgga ctcgtgctcg ccggcatgaa cgtctgctac tacgcggcgc
     7021 tccagcacct gccgatcggg gtgacggtga cgatcgagtt caccggtccg ttggccgtcg
     7081 ccgtgctcgg gtcgcgacgt cggatcgaca tcttctgggc gctcctcgcc gctgtcggcc
     7141 tggtgctgtt gagccccgtg acaggcgttg atctcccgat cgtgggggtg ctgctcgccc
     7201 tcgcggccgg agcgttctgg gccgggtaca tcctgttgag cagccgggtc ggtgagcggg
     7261 tgtcgggtct gtcgggcctc gcactcgcgc tcgtcgccag cgccgcggtg acggcgatcc
     7321 cggcggttgc cacagccggc accacgctgc tcgacccgaa ggtgatcggc atcgggttgg
     7381 tcgtggcgct catggcgtcc gccattccgt acggctgcga gctggaggcg ctgcgctcgt
     7441 tgaccacggc cagcttcggc atgctgatga gcctcgagcc ggcgttcgcc gcgttggcgg
     7501 gggccgtcgt cctgagtgaa cggctcggtg ccccggagtt gttggcgatc gggttcgtgc
     7561 tggttgcgaa cgccggggcc gtgctcaccc gccgggctcg gacacccgac gtcgacgtcg
     7621 gtatcgtgca gccgtgaccg gcattccgac cgaacggtgg actgagttcg acacccccga
     7681 cggcgaccac tggcgcgtcg acctcgggtt cctcgcttcg ggatggacgt gcatctgggg
     7741 tgccggctgc cggggcatcg ggcccgatgc ggatccgacc cacatcatgg ggtgctgctc
     7801 gttgggcgcc gagttcgccg accccgacga cgcccgcaac gtggcggccc acgctgcgtt
     7861 tctcgacccc gaacggttcc agttcgctgc ggtggccgag gccgacggca tcttcgccga
     7921 cgagtcgcga cgggcgaccc gggtggtcga cggcgggtgc atcttcctga accgccccgg
     7981 gttcgagggg ggtgccggct gcgcgttgca ccaggcggcg ctcgccgacg acgagccgcc
     8041 ccgtgactgg aagccggtcg tctgctggca gctgcccttc cggatcgacg tcgaatcggg
     8101 agtcacgatc gtgcggcgtt gggcccgggc cgacttcgac gagcccgatg atccctcggc
     8161 gtcgatcccg tggctgtgca ccgaggacga ggccgcctac ctggcggacg cgtcggcgct
     8221 cgaccgccac cgcgacgacc tggtcgagct gctcggtgaa gcggccgttg cggagatcgg
     8281 cgcgcggtgc tcagtcgacg cggaagcggg cgatgacgtc ggggtcgagt tcgacaccga
     8341 acccgacgcc tgagggcacc gcgatcgagc cgtgctcgac cacgatcggc tccgtcacga
     8401 tgtcggtgcc gtagaaccgg gcactcgccg acaggtcacc cggcagcgtg aagcccgcca
     8461 tcgccgccag cgccacgttg gcagcgcgcc ccagaccggt ctcgagcatt ccgccgcacc
     8521 acagcgggat cgactgctgg acacaccagt cgtggatgcg tcgcgcctcg aggtagccac
     8581 cgacgcggcc gggcttgagg ttcaccacat cgcacgcgcc gagggcgacg gcggtgcggg
     8641 tcgtgtcgat cgacacgatc ggttcgtcga ggcacacggg cgtgacgagt cgctcggcga
     8701 gtcgcgcatg cccgacgagg tcgtcctcgc cgaacggctg ctccacgagc agcaggccga
     8761 actcgtcgag cttggcgagg tggtcgagat catcgcggtc gtacgctccg ttggcgtcga
     8821 cctggagcgc gagtgcgggg aactggtcgc gcaccgaagc gatgtagtcg aggtcgcggc
     8881 cgggtgcgat cttgagcttg gcccgccggt agccctcgtc gacgtaggcg gcgacgcgat
     8941 cgcacagctc ggcgggggac cccgcaaagc cgatggcgac cccggcgctg acgtgggtgc
     9001 gcgtgacgcc gaggaagtcg ccgaacgacg tgccggcggc ccgaagctcg gcgtcgagca
     9061 tggccatttc cagcgccgac ttggccatgc gatgcccgtt gaccggcgac atgatcgtgg
     9121 ccgcctcgga ggcggcgatg tcggtggggg cgagcagccg ggggatcagg tggtcgcgca
     9181 gcacgatgcg ggccgaggcc acgaactcgg ccgaatagcc cggttcggcg aaggccgcgc
     9241 actcgcccca gccgtcaccg atgtcgctcc ggagccggac gagcaggacc tctcgcccgt
     9301 tgagcgtgcc cgcagcggtg tgccacggag atcgcaacgg aagccggacg gcgatcacct
     9361 cgatggattc gatgcgcacg ggcccgggtc taccaccgag tcgaggggaa gcggtgacgc
     9421 gtgacagaga accatgccgg cctcggctag aacctgttcc atgctgacga tcaccgacca
     9481 ggaccgggtc cggctcctca ccctcgaccg ccccgacgcc ctcaacgcct tcaacgaggc
     9541 gctctacgac gccgtgagcg aagcgctcat cgaggccgcc ggcgacgatg ccgtggcgac
     9601 cgtggtcatc accgggtcgg gtcgtgcgtt ctccgccggt caggacctgc tcgagatgag
     9661 cgagcgcatc accaaccccg acttcgtgca gggcacccac gggtttcccg gtctcatcga
     9721 ccagctcgcc gcgttcccca agccgttgct cctggcgatc aacgggctcg ggctcggcat
     9781 cggcaccacg atcatcgggt ttgccgacct cgtgttcatg tcgaccgacg cccgcctgaa
     9841 gtgcccgttc acatcgctcg gcgtcgcacc cgaggcggcg tcgagcctgt tgttcccccg
     9901 cttgctgggt caccagaacg cggcgtgggt actgctgagc gccgagtgga tctcggccga
     9961 ggaggcgaag gaactcgggc tggcgtggaa ggtgacgtcg cccgaggagc tcctcgacgt
    10021 caccatggcc cacgcgacga agctcgctgc gatgccgatc agctcgttga tcgccgtgaa
    10081 gggcacgatg acggccggcc gggtcgaggc gacgcgtgct gcccgtgacc tcgaggacgc
    10141 tcagttccag aagctgatgg gtgggcccgc gaacatggag gcgttggcgg cgttcgcgga
    10201 gcggcgcccg gccgacttca cgaatctgcc tcccggttgg tagtcgggag gagcaccgcc
    10261 tcgtcggctg cggcgggtac ggccgtgccg gtcgacgccg cgaacgagtg gtggaacggg
    10321 aagtggtcga gccgccggat ggccgggaac aacatcggcc agatgagcac gacggcgatg
    10381 gtcgcagcgc cgcccgccac gatcgccggc ccggctccca gcgcctgacc gagcagcccc
    10441 gactcgaacg ctcccagttc gttcgatgca ccgatgaaca cgttctcgac ggcgccgacg
    10501 cggccgcgga cctcgttggg ggtcacgagc ggcacgagcg tggagcggat gaagacgctg
    10561 atcgagtcgg cgcccgacaa cacgacgagc gccccgaacg ccacaccgaa gttgcgggtg
    10621 aagccgaaca cgatcgtcgc cgcgccgaag acgccgaccg tggcgaacag cgtggcgccg
    10681 acgtggcgtt gcaccgggcg gaacgcgagc gtggcagtca tgaggccggc gcccaacccg
    10741 gcggcggccc gcagccaccc gagcccgacg accccgacgc cgaggcgctg ctcggcgaca
    10801 gcgggcagca gggcgacggc accgccgaac agcacggcga acaggtcgag ggagatcgcg
    10861 ccgaggagca gcggctcctt tcggatgacc ttgaggccct cgagtgcttc ggacaccttg
    10921 ctggtgccgc tggtgccgct ggctcgttcg tcgcggcgag ccgactccgc ggcgcggtgt
    10981 tcgtaggtcg ggtgcaactg gacgaacagc acgaggaccg caccgacgag caggcaccct
    11041 gccgatacgg cgaagggaac ccagacgtcg atcgtgtaga gaaacgcgcc gatgaccggg
    11101 ccggcgatga tcccggcttg gaaggccccg ctgtagaggg gactgatcct cgggagcgct
    11161 tcgggggcga cgatcgtgga cggcagggcc cggatcgccg gcgagaggaa cgcccgggcg
    11221 atggcgtagc cgacgaccaa cccgaagatc gggccgatgg tcgcgtcgga gcgttcgacc
    11281 tcgacgatca gcagcagggt gaacgctgcc tcgccgacca acgcgatcga cgcgaccttg
    11341 cggcggtcga accggtcggc gacggcgccg gtcagcagca cgagcagcgc gacggggagg
    11401 aactcggcga gcccgagcag cccgaggtcg agctcacggc caccgatgtc gaacacccgc
    11461 ttgcccagcg cggtgaactg cgagctgatg ccgatctcca ggatgaacgt ggcgaggagg
    11521 aagatgacca ccgaccgctg cgtcgccacc tccttgaggc tgggcacgac ggggcgcgtc
    11581 gatgccgcgt cgtcgacggg cgcgttcact ggccgtagcg gaacatgacg aacccgccgg
    11641 tctgcggtgg gatgcagatg gcggtgagcg cggcgagcag ccaggtggct tcggcgacga
    11701 gcgaggtgcc cgccggcggt cgcacgacga gcccgcgacg ggggttgtca ctcaacaccg
    11761 cccactccgg tgggatcgac cccggcgtgt cggcgagcgc cgccctcgcc tgcttggcgg
    11821 cgccgtgttg gagcccgatc tcgaccggtg cggtcgtgcc gttgcggccg ggagtcgggg
    11881 ccgtccaggt gccgagcggt accaccgggc ctcgcttgga gaagaccgcg agcgcgctcg
    11941 gcgtggaggg gaccggcaca tcctcgggga tctcggggag gaggttgatc cagcccgatc
    12001 gggcctcgac gagctcggcc atgaccgcgg cgagggccga cgggtcgtgc gcgatcaccg
    12061 tgggggtggg gcgggtcagg gccatggtgg gccagcctac gatcttgccg tgaccaccgg
    12121 attcgcagtt tcgcccgagc acgtggtcaa cgatccggcc gacgcccgca tcggcgagtt
    12181 cgtcggcctc cgcgaccgcg atctgcgggt gcgcgatcgc gcgaccgcag ccaccgactc
    12241 gccgctcgac gcggtgttca tcgccgaggg cgacccggtc gtcgcccgcg ccgtccgggc
    12301 ggggttcgac ctggtgacgc tgctcgtcga cgcgacccgg acccgcccgt tgcccgacgg
    12361 cgtcgaggcg gccgccgggg tgatctacgg cgccggcccc gaggtgctgc aacgcatcac
    12421 cggcatgggt gtgcaccgcg ggtgtctcgg agtctttcgc cggcggccgc tgccgtcgct
    12481 cgacgaggtg ctcaccgggg cgcggcgggt gctcgtgatg gagcgggtcg tgaacccgat
    12541 caacctcggc gtgatcgccc gaacagccgt cggtctgggg atggacgcca tggtgctcga
    12601 cgacgccagc gtcgatccgc tctaccggcg ggcgtcgcga gtcgcgatgg gtgaggtctt
    12661 cgagttcccg tatgcccgtg cgggccgctt ccccgccggc ctggaagcgg tgggcgcggc
    12721 cgggttcaca ctcgtcgcgc tcacgccggc cgatggcgcc gagtcgatcg acgacatcgc
    12781 attcggcccc gacgagcggg tggcgctcgt gctggggagc gaaggtccgg gcctcaccga
    12841 cgagacgatg gagcggtgcg accgccgggt gtcgatcccg ctgtcgggca acgtcgactc
    12901 gctcaacgtc ggtgccgctg cggccattgc ctgttacgcc atcacgcggg cctgatcgag
    12961 gttctccccg gcgcggctac cgtcggccgg atgtctcggc cctggtggca caccacctcg
    13021 atctatcagg tgtatccccg ctcgttcgcc gactcgaacg gtgacggcat cggcgacctt
    13081 ccggggatcg tggggcacct cgaccatctc gtcgacctcg gggtcggtgc catctgggtg
    13141 tcgccgttct tcgccagtcc gcaacgcgac atcggctacg acatctcgga ctaccgctcg
    13201 gtcgctccgg agtacggcac gctcgacgac gcccgcacgc tgatcgacga ggcgcaccgg
    13261 cgcggtctgc gcgtcctgtt cgacctcgtg ctcaaccaca cgtccgacca gcacgcgtgg
    13321 ttccgcgaat cgcgctcgtc gaggaccaat ccgaaggccg actggtacgt gtggcacgac
    13381 ggtcgagtcg gtagcggcgg gcgccgactg cccccgaaca actggcgttc cgagctacag
    13441 ctgacgaagg cgtggcagtg gagtgacgag cgcgagcagt ggtacctcgc cacgttcctg
    13501 cccttccagc ccgatctcaa ctggcgtcac cccgacgtac gctccgagat gttcgacacc
    13561 gtcaggtcgt ggctggcgat gggggtcgac gggtttcgcc tcgacatctt cgggtcgatc
    13621 atgcaggacg atcagttgcg cgacaatcca cgccgacccg acctctcgtt cgggctgcca
    13681 cgggtccaac agccgaccaa cacgctcaac accgacgaca acttcacgct cgccggtgac
    13741 ctgcgggcgg tctgtgacga gttcaccccg ccgcccggaa cacccgagat cccgggagcc
    13801 ggcgaacgaa tcctgttggg cgaggtgttc ggcccggccg agacgcttcg ccgttacgtc
    13861 cgtgacggcg atcgcgacgg ccttcacctg gtgttcctgt tcgatttcct cacggcgacg
    13921 tactcggcgg cggggtaccg gcgcctgatc gaacggttcg aggccgagtt ccccgatccg
    13981 ctccaaccga ccta
//
LOCUS       DAOSOA010000046        13937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4416_length_13937_cov_7.072540, whole genome shotgun sequence.
ACCESSION   DAOSOA010000046 DAOSOA010000000
VERSION     DAOSOA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13937
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4416_length_13937_cov_7.072540"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1524
                     /locus_tag="PKY13_04840"
     CDS             154..1524
                     /locus_tag="PKY13_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT79287.1"
                     /translation="MTANRPLRLAAVAAALALLFTACGRDDEASDSTDDSTATTAADG
                     GTDSGTDAPKAFINPEDDCTDYQGTKGIEGNTIKIGTVRPESGPYSIYDTVTKGLEAY
                     VGSINAKGGVKAGDGKTYQLELVKADDAYDPAKTPGEVQRLVEQEGIFAMVGQIGTSN
                     NLAVRQYMNDNCVPSIGLATGSVEWGKAAEFPWFIGGLPSYATEGHAFMEYLIKNKPD
                     AKIALLYQDDDYGKAYQDVIKKDIEGTGITLVGEQSFNPLTETSPESKVAQLSGSGAD
                     VFFVGIGGTPCPAAIRAIPDTWTPMTYVSITCSGKTAMALTQGKDEGLYSAQATLDVG
                     TTADQANPKVQEFFTEGAAQGLDEATLQGGIAAAGWGFGSLFVRGLELTPTVDRATLM
                     NTLYSMKDQNYGLMLDDATTNTDGSTDPWVLEDLKIITRAGGQWNEAAPLVSYDGTSN
                     DLAGLK"
     gene            1616..1954
                     /locus_tag="PKY13_04845"
     CDS             1616..1954
                     /locus_tag="PKY13_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79288.1"
                     /translation="MTPKRVALGFLAIISGLGCLYVATTDVHYRDRNCGTALFKADTN
                     RLAVETGDVGEDQFEQESLITNCDQLILERRFLAAAPAAVTVVAVVAGRRARDAKPAI
                     PGDIFGSGRT"
     gene            complement(1956..2558)
                     /locus_tag="PKY13_04850"
     CDS             complement(1956..2558)
                     /locus_tag="PKY13_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79289.1"
                     /translation="MRRVGGCLVAVALVVAASACVSTNEVDPSGNIRTNDATPTTTAP
                     TPQAGVLGQDVTYGGVVVTVVSVAPFDQSPNGTPRIKAVMRSENVSDSAQKNPNLELI
                     CDETSNTGDWFLGSTWEPNVVLPVNVVAQGEVIVGFPLKGTNPEYPVVTCTSAKLRLS
                     VVGAREDVPVVVDYPVDASLIDQAIRRPRGPSLPLPPRGS"
     gene            complement(2558..3646)
                     /gene="rlmB"
                     /locus_tag="PKY13_04855"
     CDS             complement(2558..3646)
                     /gene="rlmB"
                     /locus_tag="PKY13_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOT79290.1"
                     /translation="MSGPKKRTGKPTGGRPGAPAKGSGPRSASGAKGAPARSSGGPKG
                     TSPKGAAPRAAGPKGTGPKRGAPAASAPTRSTSARSGPAPSRGPGPGRGKGGPRRTPG
                     APPVRSADRGLDGTQVEGRHAVRELLLAGNRKVKEILLAEQMDPAEILEDIVDLAHDL
                     KVPVREISKRRLDAESRTEASQGVVAHAEPLREHSLTDLATSVPGSPPPFLVAVDGVT
                     DPGNLGAIIRTAECAGVTGLLLPRHRAVHVTPTVTKSAAGAVEHVPMALVGGLPAAIE
                     EMKKLGVWVVGLDAGGEKSLHRVELGDEPVCLVLGAEGEGLSRLVRQRCDQIVSIPLV
                     GSLSSLNVATAAALAIYEVVRRRSESVG"
     gene            complement(3687..4826)
                     /gene="ispF"
                     /locus_tag="PKY13_04860"
     CDS             complement(3687..4826)
                     /gene="ispF"
                     /locus_tag="PKY13_04860"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOT79291.1"
                     /translation="MDHRIWSIVVAAGSGQRYGAAKQFEPLAGARVVDHSLAVAARHS
                     DGVVVVVPPGEPVPDALRADVVVEGGATRSESVRRGLAAVPGDTTIVLVHDAARPVAS
                     DALFRRVVDAVLGGAAVAVPAIEVADTIRHRDGGVVDRSELLAVQTPQGFDAAALRAA
                     HANGGEATDDASLVEARGGTVVTVAGERTNIKITEPADLVVAAQTLGTSGSSAAPPPS
                     ALRLRVGNGFDIHRFSDDPDRVLVLGGVTFEGARGLHGHSDADVVAHACSEALLGAVG
                     RGDLGSHFPDTDERWRGANSMELLAEVVRIVAADGMVALNIDCSVIAESPKLAPRRHE
                     MEAALSAVVGAPVSVKGRRAEGIGGLGRREGIAAMATALLTGAGG"
     gene            4926..5585
                     /locus_tag="PKY13_04865"
     CDS             4926..5585
                     /locus_tag="PKY13_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT79292.1"
                     /translation="MIDATMLRSIELFSDLGDEVLEQLLDQSRSLDLVRGDVIFAEGS
                     EADALYVVESGRIAIGNKSFDGRESMVALMTHGDLFGEMSLFDKLGRSAEARALEPSE
                     VVEIPYRPLIDLWEHQPELLWKVIGLLTKRLRNMDEALADSFFLDVTGRTAKHLLELA
                     GDRDEFEIPITQEELAGLVGASRERVNKAISSFVKLEWIDQNDRTYRILKRRELEIRA
                     M"
     gene            complement(5602..6267)
                     /locus_tag="PKY13_04870"
     CDS             complement(5602..6267)
                     /locus_tag="PKY13_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOT79293.1"
                     /translation="MRITLTTGTPAELARPTNGEATGGLVLWADIFGLRPLFDGHAQR
                     LADDHGWVVVAPELYPGEETMPIEARHARAATFSDADKLADAVAAADATGFEHVGVLG
                     FCMGGMYAMKSLASNRFSRAVAFYGMVRVPEQWSGGPQGDAIDVVNERRERGDLHLLG
                     IFGTNDPWCPLDQIDELEGAGAEVVRYEGADHGWAQDSTRDNYREADATDAWARAEAF
                     LEG"
     gene            complement(6288..7361)
                     /gene="disA"
                     /locus_tag="PKY13_04875"
     CDS             complement(6288..7361)
                     /gene="disA"
                     /locus_tag="PKY13_04875"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HOT79294.1"
                     /translation="MAARRSKALLEALAIVAPGQPLREGLDRVVKASMGALIVVGDGP
                     EVLNICSGGFLLDAAFSPQRLSELAKTDGAIILAPDASRIARANVHLVPNPNTPTTET
                     GTRHRTAERVARSIGVPVISVSEDMAVLTVYMHDDKHQLEPIPSVLSRCNQALQTLER
                     YKNRLNEVTSNLATVEVEDLVTVRDVVTLLQRTEIVVRIAEEIQRYLVELGSDGRLVR
                     LQLRELMAGVEDDRKMVLLDYFQPDASWNLEQAMETLSDLEMEDLLEPEAVAEALHLS
                     GSAKDADGSLAPRGYRMLSKVPRLPEGLAERIVEQFQSLDKLMRATIDELAAIDGVGD
                     QWARTIKDSLGHIAESSILDRYS"
     gene            7492..8739
                     /locus_tag="PKY13_04880"
     CDS             7492..8739
                     /locus_tag="PKY13_04880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT79295.1"
                     /translation="MPTDAPSRSMLDRMVDATPPGRDRYVDLLRAVSITVVVLWHWVG
                     SVTHWTASGRLSMPNPIEELPAGWAATWVLQVIPVFFVVGGYANFVSLRSARTAGRTA
                     GQFVRSRMERLLRPVVPLAMTWVALDVAGRALVPGYTGVLHWGVVVFVPLWFLAVYAG
                     VVALAPLTAALHQRAPRSTIVALAAAIALVDVSRFRLDAPAVGYLNGGLVFVFAHQLG
                     YWYADGTFTTWSRRRLLGLTTGALTGLAVLTSWGPYPASMVATPGAPLSNLNPPTAAI
                     ACLAVFQISVALLLRPAANRWLARRGPWRAVIAANSVIMTVFLWHMTAYVAAVAVVEA
                     LGGELGNEATTEWWLQRPLWLVLPGLALAPMVRTFRTWERPRPAPVSLAQAQEVDCGV
                     DGGHTEHLQTDVDLGSRARHDRS"
     gene            complement(8632..9999)
                     /gene="radA"
                     /locus_tag="PKY13_04885"
     CDS             complement(8632..9999)
                     /gene="radA"
                     /locus_tag="PKY13_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOT79296.1"
                     /translation="MALTTSQPRRKTRYACIECGTGAPKWSGKCDGCGEWNTIVEEPV
                     DADPVVVVAPAVPARPITEVSSLDANPLPTGIAEFDRVLGGGLVPGSVTLVGGEPGVG
                     KSTLLLQVLAEPARSGATVLYLSAEESTQQVRRRAERLGALHERLYLAAETDLSRVLG
                     HVDQLRPSVVVIDSIQTVFDPELSSAPGSVGQVRQCAHRLVRLAKERDIAVILVGHVT
                     KEGALAGPRVLEHVVDTVLSFEGDRHHTLRVVRATKHRFGSTQELGLFAMGEGGLEAV
                     ADPSEMFLADRRPGIAGSVIVPTLEGHRPLVVELQALVAASGGGHPRRSVTGVDPGRV
                     GLLLAVLERRVNVPVLGLDTYAMAVGGARVTEPGADLGLALAIVSSATNVAVPADVVA
                     VGEVGLGGELRSVGGIERRLAESARLGFSRAIVPRSTPEVDVGLQVLRVPTVHAAVDL
                     LGLRE"
     gene            complement(10117..11313)
                     /locus_tag="PKY13_04890"
     CDS             complement(10117..11313)
                     /locus_tag="PKY13_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOT79297.1"
                     /translation="MLRRQAVLIPGGALVGLTAGAASAGFLRSLEWATEAQASNKWFL
                     WLLPVAGATMATITARYGGRASAGNRLTMTQIHAFGDGVPLRMAPLVYVGTIVTHLFG
                     GSAGREGTALQMAAGLTDGVGARLRVSSEDRRLLLIAALAAGFGSVFGVPWAGIVFAV
                     EVAPTAWRQRVVALPAVVVAAVLGDRVVGWLAVHHTAYPEITGIRWRDALIVALVAPA
                     FGIAAWGFIRLTEGIKEQLTSRLEVPAVRALVGGAVVIALTLLVGNRDYNGLSLPLLG
                     RAFGLVDIGLGVWALKLLFTAVTVGSGYAGGEVTPLFVIGATLGAAVAHATGGPVLVL
                     AAVGMMATFGSAVNASTACVVMGIELFGWGPVVPLIVGCGIARLCSSGRTLYAHETVP
                     VEAELL"
     gene            complement(11318..12112)
                     /locus_tag="PKY13_04895"
     CDS             complement(11318..12112)
                     /locus_tag="PKY13_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79298.1"
                     /translation="MFRPVRVLVPLLLALSVVGCRARADVLVAVEPDGSGTVTVTATL
                     DPDAVAQVGDVAAALKVDDLRSAGWKVTGPKKAGADTVITVSKPFAEASRLGAVLEEL
                     GGPDGPFPGWKLTSSNSFTVSSYELTGKIRLTGSLDQFSDGEVAAALDGFATGRSPEE
                     LTAALQDDPDALTLNVAVTLPGDLDDVSGLDRTDPAPNVAAEFFRLGDGRAVDTAVEL
                     RSSSTDRSAVLWVILGGGLIVASALLFVAGRQQARRRRGRRPAAAE"
     gene            complement(12211..>13937)
                     /locus_tag="PKY13_04900"
     CDS             complement(12211..>13937)
                     /locus_tag="PKY13_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT79299.1"
                     /translation="IKTRGDIILFIDEIHTLVGAGAAEGAIDAASILKPMLARGELQT
                     IGATTLDEYRKHLEKDAALERRFQKVVVDEPSVAHTIEILKGLRERYENHHRVTITDQ
                     ALVAAANMADRYISDRQLPDKAIDLIDEAGARLRIRRLQSPPDYRELESEIAEVTRSK
                     KEAVEKQDFEEAGRLRDKEKELLAQKEAKDVEMKASGVDLFDEVDEEAIAEVLSLWTG
                     IPVYKLTEEETAKLLRMEDELHRRVIGQNEAIKAVSQAIRRTRAGLKDPKRPSGSFIF
                     LGPSGVGKTELAKTLAEFLFGDETSMITLDMSEYMEKHTVSRLVGSPPGYVGYEEGGQ
                     LTEAVRRKPFSVVLFDEIEKAHPDVFNTLLQILEEGRLTDAQGRSVDFRNTVLIMTSN
                     LGTADLRKAVVGFSQRNDAMSYEKMKEKVNEALKAHFRPEFLNRIDETIVFSELSKPE
                     VTQIVDFMIARVGVQLSGQGMGLEITEAAKLLLADRGYDPTMGARPLRRAIQRLVEDP
                     LSEKLLWKEFRAGQIIRVDVAPDPEKDGEQIFTFEGLEGFVPPTVGDVIEAELAATGG
                     GEPVADAP"
BASE COUNT         2207 a         5025 c         4706 g         1999 t
ORIGIN      
        1 gctcgttgcc ccgggcggga tcgtcggtct ctaccgacag accgtcgccc ggatcgccgg
       61 ccgccgagcc gcgtcggcga ccgccgccga cacccccgtt cccaccgcgc cctgaggggc
      121 gcccgaccca tccacctcac cgggaggacc ccaatgaccg cgaaccgacc actccgcctc
      181 gcagctgtcg ctgctgcgct cgcactgctg ttcaccgcct gtgggcgcga cgacgaagcg
      241 tccgacagca ccgacgattc caccgccacg actgccgccg acggcggcac cgactcgggc
      301 accgacgcac ccaaggcgtt catcaacccc gaggacgact gcaccgacta ccagggcacg
      361 aagggcatcg agggcaacac catcaagatc ggcacggtgc gccccgagtc cggcccgtac
      421 tccatctacg acaccgtcac caagggcctc gaggcttacg tcggctcgat caacgccaag
      481 ggcggggtca aggccggcga cggcaagacc taccagctgg agctggtcaa ggccgacgac
      541 gcctacgacc cggcgaagac ccccggcgag gtgcagcgtc tcgtcgagca ggagggcatc
      601 ttcgccatgg tcggccagat cggcacctcc aacaacctgg cagtccgcca gtacatgaac
      661 gacaactgcg tgccgtccat cggcctcgca accggctcgg tcgagtgggg caaggctgcg
      721 gagttcccgt ggttcatcgg cggcctgccg tcctacgcca ccgagggcca cgccttcatg
      781 gagtacctga tcaagaacaa gcccgacgcc aagatcgccc tcctctacca ggatgacgac
      841 tacggcaagg cctaccagga cgtcatcaag aaggacatcg aaggcaccgg catcaccctc
      901 gtcggcgagc agagcttcaa cccgctcacc gagacgagcc ccgagtcgaa ggtcgcccag
      961 ctgtccggct ccggcgccga cgtcttcttc gtcggcatcg gcggcacgcc ctgccccgcc
     1021 gcgatccgcg ccatccccga cacctggacc ccgatgacct acgtgtcgat cacctgctcc
     1081 ggcaagacgg ccatggccct cacgcagggc aaggacgagg ggctgtactc ggcccaggcg
     1141 acgctcgacg tcggcaccac cgccgaccag gcgaacccca aggtgcagga gttcttcacc
     1201 gagggtgcgg cccagggcct cgacgaggcc acgctgcagg gcggcatcgc cgcagccggc
     1261 tggggcttcg gctcgctgtt cgtgcgcggc ctcgagctca cgccgaccgt cgaccgggcg
     1321 accctcatga acacgctgta ctccatgaag gaccagaact acggcctcat gctcgacgac
     1381 gcgacgacca acaccgacgg ctccaccgac ccctgggtgc tcgaggatct caagatcatc
     1441 acccgcgccg gcggccagtg gaacgaagcg gcgccgctcg tcagctacga cggcacgtcg
     1501 aacgacctcg ccggcctcaa gtagtcaggc ccgaactcac cgctcctgtc ctgcagatcc
     1561 gcccctccag gggcgcatct gcaggacaga acgggtgagg tagcgtgatc ggccgatgac
     1621 gccgaaacga gtcgcgctgg gattcctggc gatcatcagc ggtcttggat gcctctacgt
     1681 cgcgaccact gacgtgcact atcgcgaccg caactgcggc acggccctct tcaaagccga
     1741 caccaaccgc ctcgccgtcg agacaggtga cgtgggcgag gaccagttcg agcaggaatc
     1801 gctcatcacc aactgcgacc agctgatcct cgaacgccgg ttcctcgccg ctgcaccggc
     1861 cgccgtgacg gtcgtcgccg tcgtcgccgg ccgccgggcc cgcgacgcga agcccgccat
     1921 ccccggcgac atcttcgggt cgggccgaac ctgactcagg aaccccgagg gggcagcggc
     1981 aggctcggac cccgaggccg ccggatcgcc tggtcgatca gcgacgcgtc gaccgggtag
     2041 tcgacgacga cggggacgtc ctcccgggca ccgacgacgc tcaggcgcaa cttcgccgac
     2101 gtgcaggtca ccaccggata ctccgggttc gtgcccttga gcgggaagcc cacgatgacc
     2161 tcaccctgcg cgaccacgtt gacgggaagc acgacgttcg gctcccacgt cgaaccgagg
     2221 aaccagtccc ccgtgttcga ggtctcgtcg cagatcaact cgaggttggg gttcttctgc
     2281 gccgagtccg acacgttctc cgatcgcatg accgccttga tccgcggcgt gccattcggg
     2341 gactggtcga acggtgcgac gctcacgacg gtcacgacga caccgccgta cgtgacgtcc
     2401 tggccgagga caccggcctg gggagtgggc gccgtggtcg tcggcgttgc gtcgttggtg
     2461 cggatgttgc cggacgggtc gacctcgttg gtcgacacgc atgccgacgc ggcgacgacc
     2521 agcgccaccg ccaccaggca cccgccgacc cgacgcatca gccgaccgac tcggaccgtc
     2581 gacgaacgac ctcgtagatg gccagcgccg ctgccgtggc gacgttgagc gacgagaggc
     2641 tgccgacgag tgggatcgac acgatctggt cgcaccgctg ccgcacgagc cgcgacaggc
     2701 cttcgccctc ggcgccgagg acgaggcaca ccggctcgtc gccgagctcg acgcggtgca
     2761 acgacttctc gccgccggcg tcgagaccga cgacccagac cccgagcttc ttcatctcct
     2821 cgatcgccgc gggaagccca cccacgagcg ccatgggtac gtgctcgacg gcgccggcgg
     2881 ccgacttcgt gaccgtgggg gtcacatgga ccgcccggtg ccgcggcaac aacaacccgg
     2941 tcaccccagc gcactcggcg gtgcggatga tggcgccgag gttgccgggg tcggtcacgc
     3001 cgtcgacggc gacgaggaac gggggcggcg acccgggcac cgacgtggcc aggtcggtga
     3061 gcgagtgctc acgcagcggc tcggcgtgag cgacgacgcc ctgggacgcc tccgtccggc
     3121 tctcggcgtc gagacgtcgc ttggagatct cgcggacggg aaccttgagg tcgtgagcca
     3181 gatcgacgat gtcctcgagg atctcggcgg gatccatctg ctcggcgagc aggatctcct
     3241 tcaccttgcg gttgcccgcc aacaacagct cgcggacggc gtgacggccc tccacctggg
     3301 tgccgtcgag cccacggtcg gcgctgcgca ccggcggtgc tccgggcgtg cgacgagggc
     3361 cgcccttgcc acgaccggga ccgggcccgc gcgacggtgc cgggcccgag cgggctgagg
     3421 tcgagcgcgt cggagccgac gcggcgggcg caccacgctt ggggccggtg cccttcggac
     3481 cggccgcccg cggcgccgcg cccttgggcg atgtgccctt ggggccaccg gaggaacggg
     3541 ccggcgcccc cttggccccg gacgcggaac gcggccctga ccccttcgcc ggcgcgccgg
     3601 ggcgcccgcc cgtcggtttg ccggttcgct tcttggggcc actcatcagt cactcgctcc
     3661 gtttgttcgg tcgtcgtcca ccgtgctcag ccaccggcgc cggtgagcag cgccgtggcc
     3721 atggccgcga tgccttccct gcggcccagg ccaccgatgc cctcggcccg gcggcccttc
     3781 acgctgaccg gcgctccgac cactgcggac aacgccgcct ccatctcatg gcggcgtggt
     3841 gccagtttgg gcgactcggc gatcacggaa caatcgatgt tgagcgcgac catgccgtcg
     3901 gcggccacga tccgcaccac ttcggcgagc agctccatgc tgttcgcccc tcgccaccgc
     3961 tcgtcggtgt cgggaaagtg cgaaccgaga tcgccccgac cgacggcccc cagcagcgcc
     4021 tcggagcagg cgtgggccac gacatcggca tcgctgtgcc cgtggagccc ccgggcgccc
     4081 tcgaacgtga cgccgccgag gacgagcacc cgatcggggt cgtcgctgaa gcggtggatg
     4141 tcgaacccgt tgccgacgcg gagcctcagc gccgagggcg gcggcgcggc ggacgacccc
     4201 gaggtgccga gggtctgcgc ggcgaccacg aggtcggccg gttcggtgat cttgatgttc
     4261 gtccgctcgc cggcgaccgt cacgaccgtt cctcctcggg cctcgaccag ggaggcatca
     4321 tcggtggcct cgccgccgtt ggcgtgggcg gcgcgcaggg cggcggcatc gaacccttgg
     4381 ggcgtttgga cagccagcaa ctctgaccgg tcgaccactc caccgtcgcg gtgacggatg
     4441 gtgtcggcga cctcgatcgc cgggaccgcg actgcggcgc cgccgagcac cgcgtcgacg
     4501 acccggcgga agagcgcgtc gcttgctacc ggacgagctg cgtcgtgcac gagcacgatc
     4561 gtcgtgtcac cgggcacggc tgcgagacca cgccggaccg actccgaacg ggtggcaccg
     4621 ccctcgacga ccacgtcggc ccgaagggcg tcgggcacgg gctcaccggg aggaaccacc
     4681 acgacgacac cgtcggagtg ccgcgccgcg acggcgagcg agtggtcgac cacccgggcc
     4741 ccggcgagtg gctcgaactg cttcgcagcg ccgtaccgct gccccgagcc cgcagccacg
     4801 acgatgctcc agattcggtg atccatgacc ggagcagcct accgggggat cgaccggccg
     4861 ctcgggcgtg tcccctgtca cgttccggct tccgaagcgc cccgctccac cgctagcgtg
     4921 gcggagtgat cgatgccacg atgctccgct ccatcgagct gttctccgac ctcggcgacg
     4981 aggtcctgga gcagctgctc gaccagtccc ggtcgctcga cctggtgcgg ggtgatgtga
     5041 tctttgccga gggcagcgag gccgacgcgc tgtacgtcgt cgagtccggg cgcatcgcca
     5101 tcggcaacaa gtcgttcgac ggacgcgaat cgatggtcgc gctgatgacc cacggcgacc
     5161 tgttcggcga gatgagcctg ttcgacaagc tcggcagatc ggccgaggcg cgggcactcg
     5221 agccctccga ggtcgtcgaa atcccctacc gcccgctgat cgacctgtgg gaacaccagc
     5281 cggagctgtt gtggaaggtg atcggcctgc tgaccaagcg gctgcgcaac atggacgagg
     5341 cgctggccga ctcgttcttc ctcgacgtga ccggccgcac cgccaagcac ctgctcgagc
     5401 tcgccggcga ccgcgacgag ttcgagatcc cgatcaccca agaagaactc gccggcctgg
     5461 tgggcgcatc gcgtgagcgg gtcaacaagg cgatctcgag cttcgtgaag ctcgagtgga
     5521 tcgaccagaa cgaccggacc taccgcatcc tcaagcgccg ggagctcgag atccgcgcca
     5581 tgtaggcgct ccctcggagg ctcagccctc gaggaaggcc tcggcgcggg cccaggcatc
     5641 ggttgcatcc gcctcgcggt agttgtcgcg ggtcgaatcc tgcgcccacc cgtgatcggc
     5701 accctcgtac cgcacgacct cggcgccggc cccttcgagt tcgtcgatct ggtcgagcgg
     5761 acaccacgga tcgttcgtgc cgaagatccc gagcaggtgg agatcgcccc gctcgcgacg
     5821 ctcgttgacc acgtcgatcg cgtcgccctg cgggccaccg gaccactgct cgggcacacg
     5881 aaccatgccg tagaaggcga cggcccggga gaagcggttg gaggccaacg acttcatcgc
     5941 gtacatgccc cccatgcaga acccgaggac acccacgtgt tcgaagcccg ttgcgtccgc
     6001 cgctgcgacc gcgtcggcca gcttgtcggc atcggagaac gtcgcggcgc gggcatggcg
     6061 cgcttcgatc ggcatcgtct cctcgccggg gtacagctcg ggcgccacca cgacccagcc
     6121 gtggtcgtcg gccaggcgtt gggcgtggcc gtcgaagagg gggcggaggc cgaagatgtc
     6181 ggcccacagg acgagcccac ccgtcgcctc gccgttcgtc ggacgggcga gttctgcggg
     6241 ggttccggtg gtgagggtga tgcgcacgag cgcagactag atcgcggtca ggagtagcgg
     6301 tcgaggatgg agctctcggc gatgtggccg aggctgtcct tgatcgtgcg cgcccactgg
     6361 tcgccgacgc cgtcgatggc ggcgagctcg tcgatggtcg cccgcatgag cttgtcgagc
     6421 gactggaact gctcgacgat gcgctcggcc aggccctcgg ggaggcgggg caccttggac
     6481 agcatgcggt acccacgcgg ggccaggctg ccgtcggcat ccttggcgct gccggagagg
     6541 tggagcgcct cggcgacggc ctcgggctcg aggaggtctt ccatctcgag gtcggacagc
     6601 gtctccatgg cctgctcgag gttccacgag gcgtcaggct ggaagtagtc gaggagcacc
     6661 atcttgcggt cgtcctcgac gccggccatg agctcgcgga gctgcaggcg gacgaggcga
     6721 ccgtcgctgc cgagttcgac gaggtagcgc tggatctcct cggcgatgcg cacgacgatc
     6781 tcggtgcgct gcagcagcgt gacgacgtcg cggaccgtca cgaggtcctc gacctcgacg
     6841 gtcgccagat tcgacgtgac ctcgttgagg cggttcttgt accgctcgag tgtctgcagc
     6901 gcctggttgc agcggctcag caccgacggg atcggctcga gctggtgctt gtcgtcgtgc
     6961 atgtacacgg tgagcacggc catgtcctcg gagaccgaga tcaccggcac cccgatcgag
     7021 cgggcgaccc gctcggcggt gcggtgccgc gtgccggtct cggtcgtggg cgtgttcgga
     7081 ttggggacca ggtgcacgtt ggcacgagcg atccggctgg cgtcgggtgc caggatgatg
     7141 gcgccgtcgg tcttcgccag ctccgacagg cgctggggcg agaacgccgc gtcgagcagg
     7201 aacccgcccg agcagatgtt gaggacctcg ggcccgtcgc ccaccacgat cagggcgccc
     7261 atcgaggcct tcacgacgcg atcgaggccc tcgcggagcg gctggcccgg ggcgacgatc
     7321 gccagggctt cgagcagggc tttgctgcgt cgggctgcca tctcggcagt ctagttggcg
     7381 actctcaggt tcccgccgca gaacgggcac cgaatccccc gaaccggccc caggcgagga
     7441 tgggggctcc ccccgattct tgtctcgggg ccaccccgcc atggtggatc catgccgacc
     7501 gacgccccgt cccgatccat gctcgaccgg atggtcgacg ccacgcctcc cggccgagac
     7561 cgctacgtcg acctgctccg cgccgtgagc atcaccgttg tcgtgctgtg gcactgggtc
     7621 ggatccgtca cccactggac cgcatccggc cgtctgtcga tgccgaaccc gatcgaggag
     7681 ttgccggccg ggtgggcggc gacgtgggtc ctccaggtca tcccggtgtt cttcgtggtc
     7741 ggcgggtacg cgaacttcgt gtcgctgagg tccgcccgaa cggcgggtcg gaccgccggc
     7801 cagttcgtgc gctcccgcat ggaacgcctc ctgcgcccgg tggtaccgct cgcgatgacc
     7861 tgggtggcgc tcgacgtcgc cgggcgggcg ctcgtgcccg gatacaccgg cgtcctccac
     7921 tggggagtgg tcgtgttcgt gccgctgtgg ttcctcgcgg tctacgccgg ggtcgtcgcg
     7981 ctggcgccgc tcactgccgc cctgcatcag cgggcacccc gctcgaccat cgttgcgctc
     8041 gccgccgcga tcgccctcgt cgacgtgagt cggttccgtc tcgacgcacc cgccgtcggc
     8101 tacctcaacg gcggtctggt gttcgtgttc gcccaccagc tcgggtactg gtacgccgac
     8161 gggacgttca cgacctggtc acgccgccgc ctgctcgggc tcacgaccgg agccctcacc
     8221 ggcctcgccg tcctcacgag ctggggcccg tacccagcgt cgatggtggc cactccggga
     8281 gcgccactca gcaacctcaa ccccccgacg gcggcgatcg cgtgtctcgc ggtgttccag
     8341 atcagcgtcg ccctcctgct gcggccagca gcgaaccggt ggttggcgcg acgaggcccg
     8401 tggcgcgccg tgatcgccgc caactcggtg atcatgaccg tgttcctctg gcacatgacc
     8461 gcctacgtcg ctgcggtcgc cgtcgtggaa gcgctcggcg gcgagctcgg caacgaggcg
     8521 accaccgaat ggtggctcca gcgcccactg tggctcgtgc tgcccggcct ggcgctcgcg
     8581 ccgatggtca ggacgttccg gacatgggaa cgcccacgac cggcgccggt gtcactcgcg
     8641 caggcccagg aggtcgactg cggcgtggac ggtgggcaca cggagcacct gcagaccgac
     8701 gtcgacctcg ggagtcgagc gcggcacgat cgctcgtgag aagccgaggc gagccgactc
     8761 ggcgagccgt cgctcgatgc cgccgaccga ccgcaactcg ccgcccaacc cgacctcacc
     8821 gaccgcgacg acatcggccg gcaccgcgac attggtcgcg gacgacacga tcgccaacgc
     8881 caacccgaga tcggcgcccg gctcggtcac acgggcaccg cccaccgcca tggcgtaggt
     8941 gtcgagcccg agcaccggca cgttgacacg tcgttcgagc acggcgagga gcaacccgac
     9001 ccgaccggga tcgacgccgg tgaccgaacg gcgcgggtgg ccgcccccgc tcgccgcgac
     9061 caacgcctgg agttcgacca cgagcggccg gtgcccctcg agcgtcggaa cgatgaccga
     9121 cccggcgatg ccgggtcgcc gatcggccag gaacatctcc gacgggtcgg cgaccgcctc
     9181 gaggccgccc tcgcccatgg cgaacaggcc gagctcctgg gtcgacccga accggtgctt
     9241 ggtcgcccga acgactcgca gtgtgtggtg ccgatcaccc tcgaacgaga gcaccgtgtc
     9301 gaccacatgc tcgagcaccc gaggaccggc caacgcgcct tccttggtga cgtgcccgac
     9361 gaggatgacg gcgatatcgc gctccttcgc caagcgaacc aggcgatggg cgcactgtcg
     9421 cacctgcccg accgaaccgg gcgccgacga caactcgggg tcgaacaccg tctggatgga
     9481 gtcgatcacg accaccgagg gccggagctg gtcgacatgg ccgagcacgc gggagaggtc
     9541 ggtctcggcg gccaggtaca gccgctcgtg cagtgcgccc aaccgttcgg cccgccgccg
     9601 cacctgctgg gtcgattcct ccgccgacag gtacagcacc gtcgcacccg accgggcggg
     9661 ctcggccaac acctgcaaga gcagcgtgga cttgccgacc ccgggctcgc caccgacgag
     9721 cgtgaccgac ccgggcacga ggccaccgcc gagcacccgg tcgaactccg cgatgcccgt
     9781 cggcagcggg ttggcgtcga gcgacgacac ctcggtgatc ggccgggccg gcaccgcagg
     9841 agcaacgacg accacggggt cggcatcgac cggctcctcc acgatcgtgt tccactcgcc
     9901 gcagccgtcg cacttgcccg accacttggg agcgccggtg ccgcactcga tgcaggcgta
     9961 gcgggtcttg cgacggggtt gcgaggtcgt cagggccatg gctcagaacc taccgagcac
    10021 cgctgacacc gagcgcggat ggtacgaaca tctgttcgtg ttctcagatc cggccggcgc
    10081 cccggacgcg tccacctggc gcgtcgagcg ccgccctcag agcaactcgg cctcgacggg
    10141 caccgtctcg tgggcgtaga gcgtgcggcc cgacgagcac aggcgagcga tgccacaccc
    10201 gacgatcagc ggcaccacgg gaccccaccc gaacagctcg atgcccatca ccacacacgc
    10261 ggtcgacgcg ttcaccgccg acccgaacgt ggccatcatc ccgaccgccg ccagcaccag
    10321 gaccggcccg ccggtcgcgt gcgcgaccgc cgcccccaac gtcgcaccga tgacgaacag
    10381 cggcgtgacc tcaccgccgg cgtagcccga cccgaccgtg acggcggtga acaacagctt
    10441 gagcgcccac acccccaacc cgatgtcgac gagcccgaac gcccgtccca ggagcggcaa
    10501 cgagagcccg ttgtagtcgc ggttgcccac cagcagcgtc agcgcgatca cgaccgcccc
    10561 gcccaccagc gcccgaacgg cgggcacctc gaggcgggac gtcaactgct ccttgattcc
    10621 ctcggtcagc cggatgaagc cccacgcagc aatgccgaac gccggtgcga ccagcgcgac
    10681 gatcaacgcg tcgcgccagc ggatgccggt gatctccggg tacgcggtgt ggtggaccgc
    10741 gagccagccc accacccgat cgccgaggac cgcggccacc acgacggccg gcagggcgac
    10801 gacccgctgg cgccacgccg tcggcgcgac ctccaccgcg aacacgatcc cggcccacgg
    10861 cactccgaac accgacccga agccggcagc gagcgcagcg atcaggagca accggcgatc
    10921 ctccgacgac acgcgcaggc gcgcacccac gccgtcggtg agccccgccg ccatctggag
    10981 ggcggtgccc tcacgcccgg cggagccccc gaagaggtgg gtgacgatcg ttcccacgta
    11041 gacgagcggc gccatccgca gggggacgcc gtcaccgaac gcgtggatct gcgtcatcgt
    11101 gagccggttg cccgccgacg cacgaccgcc gtacctcgcc gtgatcgtcg ccatcgttgc
    11161 gccggcgacg ggcagcaacc acaggaacca tttgttcgag gcctgcgcct cggtcgccca
    11221 ttcgagcgag cggaggaagc cggccgaggc cgctcccgcc gtcagaccga cgagcgcgcc
    11281 accggggatg aggaccgcct ggcgccgaag caccgcccta ctccgcggcg gcgggacgac
    11341 ggccacgccg tcgccgggcc tgctggcggc cggcgacgaa gagcagtgcg gacgcgacga
    11401 tcaacccgcc accgaggatc acccacagca cggcggaccg gtcggtcgaa ctggacctga
    11461 gctcgacggc cgtatcgacc gctcgcccgt cacccaggcg gaagaactcg gccgcgacgt
    11521 tcggggcggg atcagtgcgg tcgagccccg atacgtcgtc gagatcgcct ggaagcgtca
    11581 cggcgacgtt caacgtgagc gcgtcgggat cgtcctgcag cgccgcggtc agttcctcgg
    11641 ggctccgccc ggtggcgaac ccgtcgagcg ccgccgcgac ctcgccgtcg ctgaactggt
    11701 cgagcgagcc ggtcagtcgg atcttgcccg tcagctcgta ggacgagacg gtgaacgagt
    11761 tggacgacgt gagtttccac cccgggaacg gaccgtcggg ccccccgagc tcctccagga
    11821 cggcgcccag ccggctcgcc tcggcaaacg gcttgctgac ggtgatgacg gtgtcggccc
    11881 cggccttctt cgggcccgtg accttccagc cggcggaccg caggtcgtcg accttcagcg
    11941 cggcggccac gtcgccgacc tgcgccactg cgtcggggtc gagcgtggcc gtgaccgtga
    12001 ccgtgcccga gccgtcgggc tccacggcca ccaacacgtc ggcgcgggcc cggcatccga
    12061 cgacactgag tgccaagaga aggggaacga ggacgcgaac gggccggaac accggacgta
    12121 cgctaccggc caccgccggc tgcatcggtc accggaacgc gcaacggcgc cccgaagggc
    12181 gccgttgcta cgaccggaat cagacgacga tcagggagcg tcggcgaccg gctcgccacc
    12241 accggtggcg gccagctcgg cctcgatcac gtcgccgacg gtcgggggca cgaagccctc
    12301 gagaccctcg aaggtgaaga tctgctcgcc gtccttctcg ggatcaggag cgacgtcgac
    12361 ccggatgatc tggccggcgc ggaactcctt ccagagcagc ttctccgaca gcggatcctc
    12421 gaccaaacgc tggatggcac ggcgcagcgg ccgggcaccc atcgtggggt cgtagccccg
    12481 gtcggcgagg agcaacttgg cggcctcggt gatctcgagg cccatgccct gacccgacag
    12541 ctggacaccc acgcgggcga tcatgaagtc gacgatctgg gtgacctcgg gcttggagag
    12601 ctcgctgaac acgatcgtct cgtcgatgcg gttgaggaac tccggccgga agtgcgcctt
    12661 gagcgcctcg ttgaccttct ccttcatctt ctcgtacgac atggcgtcgt tgcgctgcga
    12721 gaagcccaca acggccttgc ggagatccgc ggtgccgagg ttggaggtca tgatcagcac
    12781 cgtgttgcgg aagtcgaccg aacgaccctg cgcatcggtg agacggccct cttcgaggat
    12841 ctgcaacagc gtgttgaaga cgtcggggtg ggccttctcg atctcgtcga acagcacgac
    12901 ggagaacggc ttgcggcgca ccgcctcggt gagctggccg ccctcttcgt agccgacgta
    12961 gccggggggc gaaccgacga ggcggctgac cgtgtgcttc tccatgtact cggacatgtc
    13021 gagcgtgatc atggacgtct cgtcgccgaa caggaactcg gcaagcgtct tggcgagctc
    13081 ggtcttgccc acgcccgaag ggcccaggaa gatgaacgaa ccggacggac gcttgggatc
    13141 cttgaggccg gcgcgggtac gacggatggc ctggctgacg gccttgatgg cctcgttctg
    13201 accgatcacc cgacgatgga gctcgtcctc catacgaagg agcttggccg tctcctcctc
    13261 ggtgagcttg tagacgggga tacccgtcca cagcgagagc acctcggcga tggcctcttc
    13321 gtcgacctcg tcgaacaggt cgacgcccga cgccttcatc tcgacgtcct tcgcttcctt
    13381 ctgagcgagc agctccttct ccttgtcacg gagacggccg gcctcttcga agtcctgctt
    13441 ctcgacggct tccttcttgg agcgggtgac ctcggcgatc tcgctctcga gctcgcggta
    13501 atcgggcggg ctctggagac ggcggatgcg caaccgggcg ccggcctcgt cgatgaggtc
    13561 gatcgccttg tcgggcagct ggcggtcgga gatgtaacgg tcggccatgt tggcggccgc
    13621 caccagcgcc tggtcggtga tggtgacccg gtggtggttc tcgtagcgct cgcgcaggcc
    13681 cttgaggatc tcgatggtgt gagccaccga cggctcgtcg acgaccacct tctggaaacg
    13741 acgctcgagg gcggcgtcct tctcgaggtg cttgcggtac tcgtcgaggg tcgtggcacc
    13801 gatcgtctgc agctcgccgc gggcgagcat cggcttcagg atcgacgcag cgtcgatggc
    13861 gccctcggcg gcacccgcac cgacgagggt gtggatctcg tcgatgaaca ggatgatgtc
    13921 accgcgggtc ttgatct
//
LOCUS       DAOSOA010000047        13847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4449_length_13847_cov_7.982526, whole genome shotgun sequence.
ACCESSION   DAOSOA010000047 DAOSOA010000000
VERSION     DAOSOA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13847
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_13847_cov_7.982526"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PKY13_04905"
     CDS             complement(<1..899)
                     /locus_tag="PKY13_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79300.1"
                     /translation="MTTRRALMVLVGATAIWALASLVDSLSIVLTDSLSIGATVLLWW
                     GAVNQNHRGRRPWQIIAAGLTLWVAGDLIWDWYRLVSGDKPSVSPADIAYLAGYPVLA
                     AGIVTMIRHRSASHWRSGLLDAIGVALASGLAAWVFLVPVSGGGSAGEQLLGVAYPLG
                     DVVLLAALAWLILSPGIRGVPSGLMLAGFGATLLLDVALAIGTVSERTLGGWFDNAYP
                     LTYLLIALAAAHQRSGELTLPDPSPSDRLQPARVVFLGIALFSGPVIGTFTDSHGGVH
                     RIAVLVTTIGIGTIVLTRFLTALR"
     gene            complement(978..3107)
                     /locus_tag="PKY13_04910"
     CDS             complement(978..3107)
                     /locus_tag="PKY13_04910"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT79301.1"
                     /translation="MNAVRRLLRPLRAHPVLSSAIGVALVAVGGYVAWQVGDLVDPEY
                     AEVTFAAPTAPKLNPESGETIYRIDPTRSEARYEIGEKIFGADASTAIGRTNGIAGDF
                     AINNDTPSKSRVGEIVINLEQLTSDSSLRDARIRSDYLESHQFKLARFTTTELAGLPD
                     SIAEGRGYNVDIIGDLTVKSTTRRVTWTATARRSGDELEVDATTTIKLSEFGMAPISI
                     VGLVSTDDEVKLGLKVTAVDPSETDVPTEIPDLTKVASTTKSPSYSREVEPILEQNCA
                     SCHNSGTIGADVLRLDNAGDASTYASGIALVTRTGYMPPWPASDVGVPLEHPRELDEA
                     SLKVLADWAAGGGKLDVKRSTALDAPAPDPSQEIRSDAVLTMDEPYVGDGTKTNDYRC
                     VIFDPGFTEPTYVTGYSFVPGTLEVVHHGLVYRVDAADRDATLAKSGVDGRTGWECNL
                     GGGVAGSMGRAANGDGGPNRGALFAGWVPGQRPIHYDADQGFLFNPGELIVAQIHYHY
                     GDKVLADSSKLVLQTEAPNPAMRELVVNNPVGPVELPCPADRADGPLCNRDAALDNLT
                     QLYGPGTSLLPNALNRLCGPTATTSDPVTGNGTTTCDRKVGRDGSVVDVLGHMHTMGK
                     SFRMTLNPGTPGETVLLDIPVWNFDWQLNYQPVDPVPVKRGDTIRIECSWDRSLRYDP
                     EPRYLVFAEGTEDEMCFSTYTVLPNQP"
     gene            3199..4641
                     /locus_tag="PKY13_04915"
     CDS             3199..4641
                     /locus_tag="PKY13_04915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT79302.1"
                     /translation="MYPERLPVDESGRLDALRRLDLIGSPPEPAFDRLVRLATTLLDV
                     PIAWISLVDADRSWFKARVGLDLGETSRDAAFCSHTIAEDDGDLFVVPDTWMDERFEN
                     NPLVIGAPRIRFYAGRAVHGPDGQPVGTLSVVDREPRDLSSRERLALLDLATLVEELI
                     APRAASDPQLAVRLESELEAARLMLEYTADVIVVLDQEAQVKFASPSFERQLGHTAAF
                     RHPEGALALFHGEDRAVARARITAAMADDAVAEAFNLRVITAAGSIRNMECTAQSLFA
                     TRSVGGVVLTMRDVSDRHMLSQMLAFQSTHDRLTELPNRMLFEEHLTPALARSRRDRR
                     PIAVCALDIAGFQELNDEFGHAAGDELLVDVAKRIRSSIRNGDSAARLGGDEFAVLLD
                     PVTDAVEARRVAERLVASISGPHSLRAGVSDCGANAGVAISTDTDDATSIIARASRAL
                     SQAKRTGAGHVVLAPTGGQLVDAAHHDLEG"
     gene            complement(4654..5277)
                     /locus_tag="PKY13_04920"
     CDS             complement(4654..5277)
                     /locus_tag="PKY13_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79303.1"
                     /translation="MSDRPPLAAPVQGWVQFAGDPAWSAARFATSISGRHHHLHIERN
                     GAGAVSIDLADVTALDELPDRPDGQQHLEIEVQRSPAVQVVWPPAFTDLVLTSLRATL
                     ETQDESAAAVPERGRRAHGALIGALALVGCGAIVLGALGLSDPEPSRPVETSVLTQTT
                     LRGPAETLGTTSTTSTTSAPSLDDCAAGPASPGTTIAPGEVDGCEPG"
     gene            5386..5811
                     /locus_tag="PKY13_04925"
     CDS             5386..5811
                     /locus_tag="PKY13_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79304.1"
                     /translation="MAGPTPLLLDLADFDAVGIATDIVVAVRSHAIEAGVDASAAISA
                     PESWHRVMVNCSSTGKTLLRVRFTDLTKSRANNVSKALVERGWQIDEDGDGASCRYPS
                     GVEATTVAFDALGALTLAGAPSDVRSVTASDSEGRAIAL"
     gene            5884..6453
                     /locus_tag="PKY13_04930"
     CDS             5884..6453
                     /locus_tag="PKY13_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOT79305.1"
                     /translation="MANESRSRQSRTPATPQNSATMGVILVIAAVVIAILLFNAGGGE
                     SKADDTDKTAAESATGESGSTTTSALPVAVTTPPANLEVVVGNGSGVPGRAKATAERL
                     GTVGYTNTKFVDGNPSPNTVVFFSPGHDADALALTQLLSLTDDRAQPLPAQTPLKEAV
                     PTAALTVLVGADFDPATASFTTTTVGPTN"
     gene            6528..7532
                     /locus_tag="PKY13_04935"
     CDS             6528..7532
                     /locus_tag="PKY13_04935"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOT79306.1"
                     /translation="MRVVVAPDKFRGTATAAEAAAALATGVRNAGHRPVSLPMADGGE
                     GLLDVFGGANRVTSVSGPLGDDVDAGWRFDGRTAIIEMARASGLHLVGGADDNDAVAA
                     STFGTGELITAALEAGARRIVVGVGGSATTDGGLGALRALLSSRLRGVELQVACDVRT
                     RFVDAAPQFAPQKGASPAQVELLRRRLDRLADVYLEEYGVDVRELEGSGAAGGLAGGL
                     AAVGGTLSSGFELVAEEVALDEALADADLVITGEGFVDEGSFDGKVVGGVVEWASEAG
                     VPVLVVAGEIFDRPSFEDRGVRVDGIDLVATYGRERAVGDTATVLEQAVTEWFRTADG
                     "
     gene            7554..>8200
                     /locus_tag="PKY13_04940"
     CDS             7554..>8200
                     /locus_tag="PKY13_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOT79307.1"
                     /translation="MNPFAALDATAQAELVHSGRATPVELVQAAINAAEALNPALNAI
                     IHPRYERALEEAAAVDPTLPFAGVPLVVKDLDGTLAGEPYHAGTRHLKEHGYIADVTS
                     WSFERLQAAGFVIIGKTNTPEFGLVPTTEPIAYGPSSNPWNPAHSTGGSSGGSAASVA
                     SGIVPVGHAGDGGGSIRIPASECGLVGLKPTRGRVTLGPVETEAWGGLVSRLVVTR"
     assembly_gap    8201..8300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8301..8876
                     /locus_tag="PKY13_04945"
     CDS             <8301..8876
                     /locus_tag="PKY13_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOT79308.1"
                     /translation="VVEAGPSQLDDEAFFNEMSGHFLTAYPVWVAQSVDELGALTGTP
                     ATPDTVEPLTWSLAETGRLVDGLSFANAVEGLRSLSRKVQEWWTDFDLLITATIPELP
                     PVLGQFGAEPDNPLAGVFRATPIVANTMPFNITGQPAISLPIAESGSGLPIGVQLVAA
                     FGREDRLISVGAQLEATTPWADRRPVVWAGQ"
     gene            8873..9784
                     /gene="folP"
                     /locus_tag="PKY13_04950"
     CDS             8873..9784
                     /gene="folP"
                     /locus_tag="PKY13_04950"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT79309.1"
                     /translation="MTEHSGGARRLRIGDDVHEIDRPWIMGIVNATPDSFSDAGEHQT
                     VDDRVALGRGHAADGAQVVDIGGQSGITGVPEIDPSEEIARVLPVVDGLRRESTVLTS
                     VDTYKPAVVRAVLGAGASIINDVSGLLYPGVAEICAEHRSALVLMHNRSRPKERLTDP
                     HLYTDVVDDVVSFIDEKMRLAVSLGVAEESIILDPGPDFSKTPFQTIETLRAIDRVLA
                     FGRPVLLALSRKDFVGALTRRSPRDRLAGTLAAIGSLLEHPGLILRVHDVAPVADYLR
                     VADALAGRAPVVPDLELPVELRRHRAG"
     gene            complement(9786..10703)
                     /locus_tag="PKY13_04955"
     CDS             complement(9786..10703)
                     /locus_tag="PKY13_04955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT79310.1"
                     /translation="MGDPVTTPRRFAPETEPERFRHRERSVTVCVPVFNEREGIARSI
                     EQIALLCDGDLIDRFLVLDGGSDDGTRDLATTLANDHANTSVLFVEDLATEVGPVLGK
                     GDSMWRSQAAIDTDVVVYLDADLLNVSPNMALSLAAPLVFGDEVDFTKGLFHRVTDRG
                     DPREYDGGRVTELVARPLINLVCADLARYYQPLGGQVGIRTDLLASLPVVTAFGVEIG
                     MLFDVSRRVGHDRIGEVELGDLVNSEKGDGELLPMAQQVAYTLLTRAGCGEPGWVPAV
                     RPTFDGDLRVVPGSDVVERPPWIERPGRG"
     gene            10812..11090
                     /locus_tag="PKY13_04960"
     CDS             10812..11090
                     /locus_tag="PKY13_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOT79311.1"
                     /translation="MSVDVRIPTTLRTLTGGANKVTVEGSTVSEVLAALDAAHPGFAS
                     RLLDDSGAIQRFVNVFVADDDVRFMDGVNTPVPDGAEVSIIPAVAGGC"
     gene            complement(11135..11638)
                     /locus_tag="PKY13_04965"
     CDS             complement(11135..11638)
                     /locus_tag="PKY13_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79312.1"
                     /translation="MNAALGGEAIHRLPYVLAALAVLVLLQTFWEERSTSGRWLAVSS
                     ATMIIALLASITLSVGRSEREQTERASAVPTTTEGPASAPTTLLTGTDVPAEVQGAVE
                     SRDDAQQLVTCESALEQIAAAIRQAGGTLDTLVAAPANGIDEPDVGRRLQSCNVRLRA
                     IAATLPG"
     gene            complement(11710..12336)
                     /locus_tag="PKY13_04970"
     CDS             complement(11710..12336)
                     /locus_tag="PKY13_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79313.1"
                     /translation="MTLPLEPAGPDRRARSGALLETQLMVLVAIVPVGLALLPDQSAV
                     SRSAWVLLSLTVATAFGAIGERRRRQRLLMRNAFALAIVAFGVLAGLMIRESAPVTFA
                     AITSDESATLTRESATDQGAGVPEASVQGATASAEDELADCEAALRRVEQAIRPVTVP
                     TTPTAVAGPDGRTVAERRQACADLVAVLDAAVRQSGALAHNETPDADR"
     gene            complement(12373..13836)
                     /locus_tag="PKY13_04975"
     CDS             complement(12373..13836)
                     /locus_tag="PKY13_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT79314.1"
                     /translation="MADVVIIGAGVAGLSSALTLSRTGHTVTLVERDATPLPTDAHGA
                     FEWDRRGAPQVRHSHAFLARLRNALRDRYPDVLDALFAAGATEMDFIAMLPEGMDRTP
                     HPGDDDLVALACRRTTFEWVLRRVVLADPTVSLLDGHTVTSLSWTAGSPPVVTGVVLD
                     DGAEIAADLVVAAGGRRCDVPHLLEPLDIDLEERTEDTGIIYFSRFFALNHGEDWPAQ
                     TGPIGGDLGYLKYGVFQGDNRTFSITFAVGTGDDEMRKIVLDPEHFIEVASKIPATAP
                     YVDGRSAPITDVFVMARLLNRSRRFTDADGAPKVLGFSAVGDSHTCTNPLYGRGCSLG
                     FVQAELLAASLADWPNNPVERARTYEARCVEEILPWYKASVAQDAANRADAVRTTEPA
                     VEGDAAPHDASAGDAPMSEADSMRAMMRDGLLPAIRTDPVVLRAFLRMFNLLEAPDSL
                     MKDWDVIGRVMTVYQDRDQRPPEPALGPGRAAILSAV"
BASE COUNT         2073 a         4749 c         4818 g         2107 t
ORIGIN      
        1 cggagcgcgg tgaggaaccg tgtgagcacg atcgtgccga tgccgatggt cgtcacgagc
       61 acggcgatcc ggtggacgcc accgtgggag tcggtgaacg ttccgatgac cggtccgctg
      121 aagagggcga ttccgaggaa caccacccgt gccggttgga gccggtccga cggcgacggg
      181 tcgggcaacg tcaactcgcc cgaccgttgg tgcgcggccg cgagcgcgat gagtagatag
      241 gtgagcgggt aggcgttgtc gaaccatccg ccgagcgttc gttcgctcac cgtgccgatg
      301 gcgagcgcca cgtcgagcag cagcgttgcc ccgaacccgg cgagcatcag gcccgacggc
      361 acgccccgga tgccagggga gaggatcagc cacgcgagcg ccgccaggag gacgacgtcg
      421 ccaagcggat aggcgacgcc gagcagctgc tctcccgcgg aaccgccgcc gctcaccggc
      481 accaagaaca cccacgcggc aagacccgat gccaaggcga caccgatggc atcgagcaac
      541 cccgaccgcc agtgcgaggc cgaccgatgg cggatcatcg tgacgatgcc ggcggcgagc
      601 accggatagc cggcgaggta ggcgatgtcc gccggggaaa ccgacggctt gtcgccgctc
      661 acgaggcggt accagtccca gatgaggtcg cccgcgaccc acagcgtcag cccggcggcg
      721 atgatctgcc acggccgccg tccacggtgg ttctggttga cggcgcccca ccacaagagc
      781 acggtggcgc cgatgctcag gctgtcggtg agcacgatcg acagggaatc gacgagcgag
      841 gccagcgccc agatcgcggt ggcgccaacc aacaccatca gtgcgcgccg cgtcgtcatc
      901 tcactggtat cgacctacct ggcctcggtg ttgagcgctt tgcgcgacga ggtaggccgt
      961 tgggcccacc gggccggtca gggctgattc gggagaacgg tgtaggtcga gaaacacatc
     1021 tcgtcctcgg tgccttcggc gaagacgagg taccggggct cggggtcgta tcgcaacgaa
     1081 cgatcccagc tgcactcgat gcggatcgtg tcgccgcgct tcaccgggac gggatcgacg
     1141 ggctggtagt tgagctgcca gtcgaagttc cacacgggga tgtcgaggag caccgtctcg
     1201 cccggagttc ccgggttgag ggtcatccgg aacgacttgc ccatcgtgtg catgtggccg
     1261 aggacatcca ccaccgaacc gtcgcgcccc accttgcggt cgcaggtcgt cgtgccgttg
     1321 ccggtgaccg gatccgacgt cgtcgcggtg ggcccgcaca accgattgag cgcgttcggc
     1381 agcagcgacg taccgggtcc gtacaactgg gtgagattgt cgagcgcggc gtcgcgattg
     1441 cacagcggtc cgtcggcgcg gtcggccggg cacgggagct cgacggggcc gaccggattg
     1501 ttgacgacca attcgcgcat cgcggggttg ggcgcctcgg tctgcaggac gagcttcgag
     1561 ctgtcggcga ggaccttgtc gccgtagtgg tagtggatct gcgccacgat cagctcgccg
     1621 gggttgaaca ggaacccctg gtcggcgtcg tagtggatgg gccgttgtcc gggtacccat
     1681 ccggcgaaga gcgcgccccg gttcggcccg ccgtcgccgt tcgcggcgcg gcccatggat
     1741 cccgcaacgc ctccaccgag gttgcactcc cacccggtcc gaccgtcgac cccggacttg
     1801 gcgagcgtgg catcgcggtc ggcggcgtcg acgcggtaga cgaggccgtg gtgcactacc
     1861 tcgagcgttc ccggcacgaa cgaatatccg gtgacgtagg tgggttcggt gaagccgggg
     1921 tcgaagatga cacagcggta gtcgttggtc ttcgtgccgt cgccgacgta gggctcgtcc
     1981 atcgtcagaa ccgcgtcgct tcggatctcc tgggacggat ccggcgccgg cgcatcgagc
     2041 gccgtcgaac gcttcacgtc gagcttcccg ccaccggcag cccagtcggc gaggaccttc
     2101 agcgacgcct cgtcgagttc gcggggatgc tcgagcggaa ccccgacgtc cgacgccggc
     2161 cacggcggca tgtagccggt gcgggtcacg agggcgatgc cgctcgcgta ggtcgaggcg
     2221 tcgccggcgt tgtcgagccg cagcacatcg gcgccgatcg tgcccgagtt gtggcacgag
     2281 gcgcagttct gctccaggat cggctcgacc tccctggagt aggacgggct cttcgtggtc
     2341 gacgcgacct tcgtcaggtc ggggatctcg gtcggcacgt cggtctcgga tggatcgacc
     2401 gcggtcacct tcaagccgag cttcacctcg tcatcggtgg acaccaggcc gacgatcgag
     2461 atcggtgcca taccgaactc actgagcttg atcgtggtcg tcgcgtcgac ctcgagttcg
     2521 tcgccggacc ggcgcgcggt cgccgtccac gtcacgcgac gcgtcgtcga cttcaccgtg
     2581 aggtctccga tgatgtcgac gttgtagccg cgaccctcgg cgatcgagtc cggcagcccg
     2641 gcgagttcgg tcgtcgtgaa ccgggcgagc ttgaactggt gcgactcgag atagtcggac
     2701 cggatgcgtg cgtcgcggag cgaggagtcc gacgtgagct gttcgaggtt gatgacgatc
     2761 tcgccgacgc gactcttcga cggggtgtcg ttgttgatgg cgaagtcgcc ggcgattccg
     2821 ttggtgcgac cgattgcggt cgacgcgtcg gcgccgaaga tcttctcgcc gatctcgtac
     2881 cgcgcctcgg agcgggtggg gtcgatccgg tagatcgtct cgcccgattc ggggttcagc
     2941 ttcggtgccg tcggagcggc gaacgtgacc tcggcgtact ccgggtcgac caggtcgccg
     3001 acctgccacg cgacgtaacc cccgacggcg acgagggcaa cgccgatggc ggagctgagc
     3061 accgggtggg cacgcagtgg gcggaggagg cgtcggacgg cgttcacccg gaaagtgtac
     3121 gcagaaccct tgctgaactc gtcgggccga tgggcaaagt tctgctcatc ggggggccat
     3181 tcgtgccgat ggtgatgggt gtatccggaa cgcctccccg ttgacgaatc cgggcgtctc
     3241 gacgccctcc gtcgcctcga cctgatcggc tcgccccccg agcccgcgtt cgaccggctc
     3301 gtccgcctgg ccacgacgct cctcgacgtg cccattgcct ggatcagcct cgtggacgcg
     3361 gaccgttcgt ggttcaaggc acgagtcggg ctcgatctcg gcgagacgag ccgcgacgcc
     3421 gcattctgct cccacacgat cgccgaggat gacggcgatc tgttcgtcgt tcccgacacg
     3481 tggatggacg agcgcttcga gaacaacccc ctcgtgatcg gcgctcctcg catccgcttc
     3541 tacgccggcc gcgccgtgca cggacccgac gggcaaccgg tcggcacgtt gagtgtcgtg
     3601 gatcgagagc cccgcgacct ctccagccgc gaacgcctgg cgctgctcga tctcgcgacg
     3661 ttggtcgagg aactcatcgc tccacgagcc gcgagtgacc cccagctcgc cgtccgactg
     3721 gagtccgagc tcgaggcggc acgcctcatg ctcgagtaca ccgccgacgt gatcgtggtc
     3781 ctcgaccagg aggcccaggt caagttcgcg agcccgtcgt tcgaacgaca actcggccat
     3841 accgcagcgt tccggcaccc cgagggcgcg ctcgcgctgt tccacggtga ggaccgtgcc
     3901 gtcgcccggg cgcggatcac cgcagccatg gccgacgacg ccgtcgccga agcgttcaac
     3961 cttcgagtga tcaccgcagc gggatcgatc cgcaacatgg agtgcaccgc ccagagcctg
     4021 ttcgcgacca ggtcggtcgg cggggtcgtg ctcaccatgc gcgacgtctc ggatcgacac
     4081 atgctgagtc agatgctcgc gttccaatcg acccacgacc ggctcaccga gctccccaac
     4141 cggatgctgt tcgaagagca cctcaccccg gcgctcgccc gttcccggcg ggaccgccgg
     4201 ccgatcgcag tgtgtgccct cgacatcgcg ggcttccagg agctcaacga cgagttcggc
     4261 cacgccgccg gtgacgaact cctcgtcgac gtcgccaaac gcatccggtc gtcgatccgc
     4321 aacggtgaca gcgccgcccg actcggcggt gacgagttcg ccgtcctgct cgaccccgtg
     4381 accgatgccg tcgaggcccg gcgggtcgca gaacgcctcg tcgcctcgat ctccgggccg
     4441 cattcgcttc gcgccggtgt gtcggactgc ggagcgaacg ccggggtcgc gatttcgacc
     4501 gacaccgacg atgcgacatc gatcatcgct cgagcctccc gggcgttgtc ccaggcgaag
     4561 cggaccggtg cgggccacgt cgtgttggcg ccgaccggtg gccaactcgt cgacgccgcc
     4621 caccacgacc tcgagggctg accccgttcc gggtcagccg ggttcgcagc cgtcgacttc
     4681 cccgggcgcg atggtcgtgc cgggactcgc cggaccagcc gcacagtcat cgagcgacgg
     4741 cgcactcgtg gtgctcgtgg tgctggtcgt gccaagcgtc tcggcggggc cgcggagggt
     4801 cgtctgcgtc aagaccgacg tttcgacggg ccggctgggt tcgggatcgg acaggccgag
     4861 cgcgcccagg acgatcgctc cgcacccgac gagcgcgagg gcgccgatga gcgcgccgtg
     4921 cgcccgccgt ccccgctccg gaacggccgc cgccgactcg tcttgggtct cgagcgtcgc
     4981 ccgcaacgac gtcagtacga gatcggtgaa cgccggaggc cagacgacct gcacggccgg
     5041 agagcgttgg acctcgattt cgaggtgctg ctggccatcg ggccggtccg gcaactcgtc
     5101 gagggccgtc acgtcggcga gatcgatcga gaccgccccg gccccgttcc gctcgatgtg
     5161 caggtgatgg tgacgtcccg agatcgacgt cgcaaacctc gcagcgctcc aggccggatc
     5221 gccggcgaac tggacccagc cctgcacggg agcggccagc ggcgggcgat cggacatccg
     5281 aggacgatac gccgcgcggg gagtgccgga aaacggtcgt ccttcaccga ggtggggtca
     5341 ggtgttgcgg tgcaccaccg cgccgccgtc tggcaggctg cgtccatggc cggacccaca
     5401 cccctgctgc tcgacctcgc cgacttcgac gcggtgggca tcgcgaccga catcgtggtc
     5461 gcggtgcgga gtcacgccat cgaggccggc gtcgacgcct cggcggccat ctccgccccc
     5521 gagagctggc accgggtcat ggtgaactgc agctcgaccg gcaagacact gctgcgggtc
     5581 cggttcaccg acctcaccaa gtcccgggcg aacaacgtct cgaaggcgtt ggtcgagcgg
     5641 ggctggcaga tcgacgagga cggcgacggc gccagttgcc gatacccgtc gggcgtcgag
     5701 gcgacgacgg ttgccttcga cgcactcggc gcattgacgc tcgccggagc accgagcgac
     5761 gtgcgaagcg tcacagcaag cgattccgaa ggtcgggcca tcgccctctg aacgcccaca
     5821 cgttcggact agggttgacg gcatgtcgag caccccctgc gcgcgacccc gaggagtttc
     5881 ctgatggcga acgaatcccg ctcgcgccag tcgcggacgc cggccactcc gcagaactcc
     5941 gcaacgatgg gcgtgatcct ggtgatcgcg gccgtggtca tcgcgatcct cctgttcaac
     6001 gccgggggtg gcgagtccaa ggccgacgac accgacaaga ccgccgccga gtcggcgacc
     6061 ggcgagagcg gctcgacgac cacgagtgcg cttccggtgg ccgtcacgac gccgccggcc
     6121 aacctcgagg tcgtcgtggg caacggctcg ggtgtccccg ggcgcgccaa ggccactgcc
     6181 gagcgcctcg gcaccgtcgg ctacacgaac accaagttcg tcgacggcaa cccttcgccg
     6241 aacacggtcg tgttcttcag ccccgggcac gacgccgacg cgttggcact gacccaactc
     6301 ctgtcgctga ccgacgatcg ggcgcagccc ttgccggcac aaactccctt gaaggaagcc
     6361 gtgccgaccg ctgcgctcac cgtgctcgtc ggcgccgact tcgatccggc gaccgcgtcg
     6421 ttcacgacga ccaccgtcgg cccgaccaac tgacccgccg tctcaccgac gcactcacgc
     6481 cccggcctgg cgcggggaga acaccgtcgg gcccgtaccc tgacacgatg cgcgtcgtcg
     6541 ttgcccccga caagttccgc ggcaccgcga cggcggccga ggctgcggcc gcgctcgcga
     6601 cgggtgttcg gaacgctggg catcggccgg tgtcgctccc catggccgac ggcggcgagg
     6661 gtctcctcga cgtcttcggc ggcgccaacc gggtgacgtc ggtgtcaggg ccgctcggcg
     6721 acgacgtcga tgccggctgg aggttcgacg gacgcacggc gatcatcgag atggcacgcg
     6781 catcgggcct gcacctcgtc ggcggcgccg acgacaacga tgccgtcgcg gcgtcgacgt
     6841 tcggcacggg tgagctcatc accgcggcgc tcgaggcggg cgcccgccgg atcgtcgtcg
     6901 gggtcggcgg gtcggccacg accgatggcg gcctgggtgc gctgagggcg ctgctgtcga
     6961 gtcggttgcg gggcgtcgag ctgcaggtcg cgtgcgacgt acgcacccgg ttcgtggacg
     7021 ccgctcccca attcgcgccc cagaagggag cgagccccgc ccaggtcgag ctgcttcgcc
     7081 gtcgcctcga tcgtctggcc gacgtgtacc tcgaggagta cggcgtcgac gtgcgcgaac
     7141 tcgaggggag tggcgccgcc ggcggcctgg cgggaggcct ggccgcggtc gggggcacac
     7201 tctcgtcggg gttcgagctg gtggccgagg aggtcgcgct cgacgaggcg ctcgccgacg
     7261 cggacctcgt gatcaccggc gagggtttcg tcgacgaggg atcgttcgac ggcaaggtcg
     7321 tcggtggtgt cgtcgagtgg gcatcggaag ccggcgttcc cgtcctcgtc gtcgccggtg
     7381 agatcttcga ccgcccgtcg ttcgaggatc gaggtgtgcg tgtcgacggc atcgacctgg
     7441 tcgcgaccta cggccgcgag cgtgccgtcg gcgacactgc gaccgtcctc gagcaggcgg
     7501 tcaccgagtg gttccgaacg gcagatgggt gattgcgggc ctacgatccc cgcatgaatc
     7561 ccttcgccgc gctcgacgcc accgcccaag ccgaactcgt ccactccggt cgggccacgc
     7621 ccgtcgagct ggtgcaggcg gcgatcaacg ccgcggaagc gctcaatcct gcgctcaacg
     7681 cgatcatcca cccccgctac gagcgggcgc tcgaggaagc ggcggccgtc gatccgaccc
     7741 tcccgttcgc cggtgtgccg ttggtcgtga aggacctcga cggcacgctc gccggtgagc
     7801 cgtatcacgc cggcacccgg cacctgaagg aacacggcta catcgccgac gtgaccagct
     7861 ggagtttcga gcgcctccaa gccgctgggt tcgtgatcat cggcaagacc aacacccccg
     7921 agttcggcct cgtgccgacc accgaaccga tcgcctacgg gcccagctcc aacccctgga
     7981 acccggccca ctcgaccggc ggctcgtcgg gcggctcggc ggcgtcggtg gcatcgggca
     8041 tcgtgccggt cggccacgcc ggcgacggcg gtggctcgat ccgaatccct gccagcgagt
     8101 gtgggctcgt cgggctcaaa ccgaccaggg gcagggtcac gctcggaccg gtggagaccg
     8161 aggcgtgggg cgggctcgtc tcgcgcctcg tggtgacgcg nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn gtcgtcgagg ccggcccgtc gcagctcgac gacgaggcgt
     8341 tcttcaacga gatgagtggt cacttcctga ctgcgtaccc ggtgtgggtc gcccagtccg
     8401 tcgacgagct cggtgcgctc acgggcacac ccgccacgcc cgacacggtc gagccgttga
     8461 cgtggtcgct cgccgagacc ggtcggctgg tcgacggcct gtcgttcgcc aacgccgtgg
     8521 aggggttgcg ttcgctcagc cgcaaggtgc aggagtggtg gaccgacttc gacctcctga
     8581 tcacggccac catcccggag cttccgccgg tgctcggcca gttcggtgcg gagcccgaca
     8641 accccctcgc cggtgtcttc cgtgccacgc cgatcgtggc caacacgatg ccgttcaaca
     8701 tcacgggtca accggcgatc tcgctgccga tcgccgagtc cggatccgga ctcccgatcg
     8761 gcgtgcagct cgtcgctgcc ttcggtcgcg aggaccgcct gatctcggtc ggcgcgcagc
     8821 tcgaggccac gacgccgtgg gccgaccgtc gtccggtggt gtgggcgggc cagtgacgga
     8881 acactccggc ggagctcgac ggctccggat cggcgatgac gtccacgaga tcgaccggcc
     8941 ctggatcatg ggcatcgtca acgcgacgcc cgactcgttc tccgacgccg gcgagcatca
     9001 gaccgtcgac gatcgcgtcg ccctcggccg cggtcacgcc gccgatggag cgcaggtggt
     9061 cgacatcggc ggccagtcgg gcatcaccgg cgttcccgag atcgatcctt cggaggagat
     9121 cgcccgggtg ctgcccgtcg tggacggcct ccgacgcgag tcgacggtcc tgacgtcggt
     9181 cgacacctac aagccggcgg tcgtccgggc cgtgctcggc gccggtgcct cgatcatcaa
     9241 cgacgtcagt ggcctgctgt accccggggt cgccgagatc tgcgctgagc atcggtccgc
     9301 actcgtgctc atgcacaacc gttctcgtcc aaaggaacgc ctcaccgacc cgcacctcta
     9361 caccgacgtc gtcgacgacg tcgtgtcgtt catcgacgag aagatgcgcc tcgccgtgtc
     9421 gctcggggtc gcggaggaat cgatcatcct cgaccccggc cccgacttct ccaagacgcc
     9481 gttccagacg atcgagacgt tgcgggccat cgaccgggtg ctcgcgttcg gtcgaccggt
     9541 cctgttggcc ctgtcgagga aggacttcgt cggcgcactc acccggcgct cgccacggga
     9601 tcggctggcc ggcacgctcg ccgcgatcgg atcactcctc gaacacccgg gcctgatcct
     9661 gcgggtgcac gacgttgcgc ccgtcgccga ctacctccgg gtggccgatg cgcttgccgg
     9721 tcgagcgccg gtcgtccccg acctcgagct cccggtggaa ctccgccggc accgcgccgg
     9781 atagctcaac cgcgcccggg gcgctcgatc cacggcgggc gctcgacgac gtcggatccc
     9841 ggaaccaccc ggaggtcgcc gtcgaaggtc ggacgtacgg ccggcaccca tccgggttcg
     9901 ccacagccgg cgcgggtcaa gagcgtgtac gccacctgct gcgccatcgg caacaactct
     9961 ccgtcgccct tctccgagtt gaccaggtcg ccgagttcga cctcgccgat gcggtcgtgg
    10021 cccacccggc gggagacgtc gaacagcatg ccgatctcga ccccgaaggc ggtgacgacg
    10081 ggcaacgacg cgagcaggtc ggtgcggatg ccgacttggc cgccgagcgg ttggtagtac
    10141 cgcgcgaggt cggcgcacac gaggttgatg agcggccggg caaccaactc ggtcacccgc
    10201 ccgccgtcgt actcccgggg gtcgccacgg tcggtgacac ggtggaacag acccttggtg
    10261 aagtcgacct cgtcgccgaa cacgagcggc gccgccagcg acaaggccat gttgggtgac
    10321 acgttcagga ggtcggcatc gaggtatacc acgacgtcgg tgtcgatcgc agcctgcgag
    10381 cgccacatcg agtcgccctt gccgaggacc ggccccacct cggtcgcgag gtcttcgacg
    10441 aacagcaccg aggtgttggc gtggtcgttc gcgagcgtcg tcgccagatc ccgtgtgccg
    10501 tcgtcggagc cgccgtcgag cacgaggaat cggtcgatca ggtcgccgtc acacaggagg
    10561 gcgatctgtt cgatggaacg agctatgccc tcccgttcgt tgaacacggg gacgcacacc
    10621 gtgacgcttc gctctcgatg tcgaaaccgc tccggctcgg tctcgggagc gaagcggcgt
    10681 ggtgtcgtga ccgggtcgcc catcgggcga gcacgctacc gggaacaaga ggggccgccg
    10741 gaggtgttgt gagacacggc cgtcatctac gatccgcgcg gcccgtgatc cacaggaaag
    10801 tagtcagcca gatgagtgtc gacgttcgaa tccccaccac cctccgcacc ctgacgggcg
    10861 gcgccaacaa ggtgaccgtc gaggggtcga ccgtgagcga ggtgctcgcc gccctcgacg
    10921 cagcgcatcc ggggttcgcg tcgcgcctgc tcgatgacag cggtgccatc cagcgcttcg
    10981 tgaacgtgtt cgtcgccgac gacgacgtcc gtttcatgga cggcgtcaac accccggtgc
    11041 ccgacggtgc cgaggtctcg atcatccccg ccgtcgccgg cggctgctga ccgggcagtt
    11101 cgacgctgcc ctgttcgggc agtcctgcgc tgccctatcc gggcagtgtc gcggcgatcg
    11161 cccggaggcg gacgttgcag ctctgcagac gccggccgac gtcgggttcg tcgatcccgt
    11221 tcgccggcgc cgcgaccagg gtgtcgagcg tgccgccggc ctgacggatc gcggcggcga
    11281 tctgctcgag cgccgactcg caggtcacca gctgttgcgc gtcgtcccga ctctcgacgg
    11341 cgccctgcac ctcggccggc acgtcggtcc cggtcaggag cgtcgtcggc gcggaggcgg
    11401 gtccctccgt ggtggtcggt accgccgatg cccgctccgt ctgctcgcgc tcggagcgtc
    11461 cgaccgacaa cgtgatcgac gccagcaggg cgatgatcat cgtcgcgctg ctgaccgcga
    11521 gccagcggcc gctggtcgac cgttcctccc agaaggtctg caggaggacg agcacggcga
    11581 gtgctgcgag cacgtagggc aatcggtgga tcgcctcacc gccgagcgcg gcgttcatcc
    11641 cgactccaac gacacatgcc gcgacgatgc cgcctcggcg tcggagcgtg ctggtcggga
    11701 gtgccatacc taccgatcgg cgtcgggcgt ctcgttgtga gcgagcgccc cactctgccg
    11761 cacggcggca tcgaggacgg cgacgaggtc ggcgcacgcc tggcggcgtt cggcaacggt
    11821 gcggccgtcg ggcccggcga cggccgtcgg cgtggtgggc accgtcaccg gccggatggc
    11881 ctgctcgaca cgacggaggg cggcctcgca gtccgcgagt tcgtcctcgg cgcttgccgt
    11941 ggccccctga acgctggcct ccggaacgcc ggctccctga tcggtggccg actcgcgggt
    12001 caacgtggcc gactcgtccg acgtgatcgc ggcgaaggtc accggcgcgg attcacggat
    12061 catgagcccg gccagcacgc cgaacgcgac gatcgcgagg gcgaacgcgt tgcgcatcag
    12121 gagtcgctgg cggcggcggc gctcaccgat cgcgccgaac gcggtggcaa cggtgaggct
    12181 gagcagcacc catgccgacc gcgagaccgc gctctgatcg ggcaggagcg ccagcccgac
    12241 cgggacgatt gccaccagca ccatgagttg cgtctcgagg agagcgcccg agcgcgcccg
    12301 gcgatcaggt ccagcgggct ccaggggaag cgtcatgaca tcccatcggc acgtcccccg
    12361 aacgtgtgag gttcagacgg cgctcagtat cgcggcgcgc cccgggccga gggccggttc
    12421 gggcggacgc tggtcgcggt cctggtacac ggtcatcacc cgaccgatga cgtcccagtc
    12481 cttcatcagc gagtcggggg cctcgaggag gttgaacatc cgcaggaagg cgcgaagcac
    12541 gacggggtcg gttctgatcg cggggaggag gccgtctcgc atcatcgctc gcatggaatc
    12601 ggcttccgac atcggcgcgt cgccggcaga tgcgtcgtga ggagctgcgt ccccctcgac
    12661 cgcgggctcg gtcgtgcgca ctgcgtcggc gcggttggcg gcgtcctggg cgaccgacgc
    12721 cttgtaccag ggcaggatct cctcgacgca ccgcgcctcg tacgtgcgtg ctcgttcgac
    12781 cgggttgttc ggccagtcgg cgagcgacgc agcgagcagc tcggcctgga cgaaccccag
    12841 cgaacagccg cgcccgtaca gcggattggt gcacgtgtgg ctgtcaccga ctgcgctgaa
    12901 cccgagcacc ttgggagcgc cgtcggcgtc ggtgaatcgg cggctgcggt tgaggaggcg
    12961 ggccatcacg aacacgtcgg tgatcggtgc ggagcggccg tcgacatagg gcgcggtcgc
    13021 cgggatcttg gaggcgacct cgatgaagtg ctccgggtcg aggacgatct tccgcatctc
    13081 gtcgtcgccg gtcccgaccg cgaaggtgat cgagaacgtg cggttgtcgc cttggaagac
    13141 gccgtacttg aggtagccga ggtcgccacc gatcgggccg gtctgggccg gccagtcctc
    13201 cccgtggttc agggcgaaga accggctgaa gtagatgatc cccgtgtcct cggtgcgttc
    13261 ctcgaggtcg atgtcgagcg gttcgaggag gtgggggacg tcgcagcggc ggccgccggc
    13321 ggcgaccacg aggtcggcag cgatctcggc gccgtcgtcg agcacgaccc cggtcacgac
    13381 cggcggcgac ccggcggtcc agctgagcga cgtcacggtg tggccatcga gcaggctcac
    13441 cgtcggatcg gcgagcacga cgcgccggag cacccattcg aacgtcgtac gccggcaggc
    13501 gagtgcgacc aggtcgtcgt ctccggggtg cggggtgcgg tccatccctt cggggagcat
    13561 cgcgatgaag tccatctcgg tggccccggc ggcgaagagg gcgtcgagca cgtcggggta
    13621 gcgatcccgc aacgcgttgc ggagtcgggc gaggaacgcg tgggagtgac gcacctgggg
    13681 cgctccgcgg cggtcccact cgaacgcccc gtgggcgtcg gtcggaagcg gcgtggcgtc
    13741 gcgctcgacg agcgtgaccg tgtgaccggt gcgcgacagg gtgagcgcgc tgctgagccc
    13801 ggccacaccg gccccgatga tgacaacgtc ggccacctgc cccctcc
//
LOCUS       DAOSOA010000048        13835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4454_length_13835_cov_6.697097, whole genome shotgun sequence.
ACCESSION   DAOSOA010000048 DAOSOA010000000
VERSION     DAOSOA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4454_length_13835_cov_6.697097"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PKY13_04980"
     CDS             <1..1093
                     /locus_tag="PKY13_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79315.1"
                     /translation="LQNLAWISFVTGRIVDAESRLGEAFEAFTEIDDQTGLAWTEGLL
                     AFVRFYQGRFAEAGELASRILRESERRNDRWAQAMMLIVIGAIDLWEGRTGAALEVAN
                     RSVSLFTSIGDPTGLQQALTLSGRGHLMSGYITEGLEMVDRAVGDLTIDTIGPQSMMS
                     VASSIAARVQLGVEQAFDPLTVAKLIQNDATGERPAEFVAAVSLALVQAGHLDAAVEL
                     LAVDHDAQRFADRKGSVLNGYALSSRALIAAIQGDHEAVAAIDAQVGDDSTYLDRAMV
                     KIALGLGRASSWIDDFAHARAEVDGTGDRLTRATIDLARASVAEAVGHDADVTWRDEA
                     EASWTELGVDPVGWRLVFSLATATAPAGA"
     gene            complement(1199..1834)
                     /locus_tag="PKY13_04985"
     CDS             complement(1199..1834)
                     /locus_tag="PKY13_04985"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT79316.1"
                     /translation="MTVRLGDDAPDFTAETTQGTINFLDWKGDSWAILFSHPKDFTPV
                     CTTELGAVAKLKPEFDKRNVKVIGLSVDELGNHTEWAKDIAETQGAEVNFPIIADGDR
                     KVSDLYDMIHPNANDTLTVRSVYIVGPDNKVKLQLTYPASTGRNFDEILRVVDSLQLT
                     AKFSVATPANWNEGDEVIIGSGVTDEAAKEKFPEGWRAEKPYLRYVPQPQG"
     gene            2108..2437
                     /locus_tag="PKY13_04990"
     CDS             2108..2437
                     /locus_tag="PKY13_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79317.1"
                     /translation="MTTPVLDKLTDVQSSVVGYISTVKEPITTGVSTVVEFVIDRFPS
                     IPAVPYADQIPTPKELIDNQYKFAKSIIDTNKDIALAVAKAAAPLTDVVLDRKPVAKK
                     VAAKKTA"
     assembly_gap    2668..2767
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2768..3412)
                     /locus_tag="PKY13_04995"
     CDS             complement(<2768..3412)
                     /locus_tag="PKY13_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79318.1"
                     /translation="MDSPVALAELARLIEDRRVGDRPFVVGITGSVAVGKSTLADRLA
                     ETITGGAPTVSTAVVSSDSFLLPNAVLADRGLVMQKGYPASFDLDALGSFLRRVRAGA
                     NDLVVPVYDHFTYDIVDGATHALPTADVVVIEGLALLGAPEIVELLDLSVYLDADVTD
                     IEGWYVHRFVQHALRANDEPGGFWDLFAGMDAERLRESALFTWTAINLPNLLDHV"
     gene            complement(3452..4669)
                     /gene="lhgO"
                     /locus_tag="PKY13_05000"
     CDS             complement(3452..4669)
                     /gene="lhgO"
                     /locus_tag="PKY13_05000"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOT79319.1"
                     /translation="MSNNAPSTPDLDVVVVGAGIVGLAAARAVQTNRPGTRVTVVEKE
                     PKVAAHQTGRNSGVIHSGIYYPPGSNKANMVAAGRSMLFDFCERHDIPTDVCGKVIVA
                     TRVDELERLRGLEARGKQHGLNTTWLTRAELREREPNVEGLGALLVPEAGITDYVAMC
                     RALVTEIEANGGEVHFDTPVESITEATDGVTLRTSKGALRARTMINCSGLHSDRVAKL
                     AGRQVKARIMPFRGEYYELTPSARSLVKHLVYPVPDPRFPFLGVHFTRMIDGEIHAGP
                     NAVVALAREGYTWRVANRIDLKEMAFDKGSWILAKKYWRTGAGEMVRSLSKAAFVRAL
                     QNLVPAVTANDLIKSKAGIRAQAIRPDGTLVDDFEFADGPRSVHVVNAPSPAATASLA
                     IGEEIANRWSKLD"
     gene            complement(4666..>5607)
                     /locus_tag="PKY13_05005"
     CDS             complement(4666..>5607)
                     /locus_tag="PKY13_05005"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOT79320.1"
                     /translation="ELGDDMHRRVIGSLMVAVVALVSACAPEIPTRATTVRTVTPLPT
                     ECPANAPTVAPQSITALGRGSALIAPADYEPGVAYPLLVSLHPFILAPDAWEAYSGLA
                     AAASQRGYWVLLPQGSDPGPRWAVPGGLDTGPDDIAWVDALIRTTADTVCVDGDRVFA
                     AGFSAGAAMSVAVSCELPGRFRGIAGSGGTNLTSLCPDGAPVDTMILHGSADPIAPVT
                     GSETVFAPPLGLTVDAVVASFAGRNGCVGPPAAVTLTSSVTIDRYSCGAHRLEYYRML
                     GAGHTWAGSPIPLDIVTGPTDHSFSATNVVLDFFGAS"
     assembly_gap    5608..5707
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5708..5890
                     /locus_tag="PKY13_05010"
     CDS             <5708..5890
                     /locus_tag="PKY13_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79321.1"
                     /translation="VDPADSERLQKGAGVAAMEMRGRSMTGWLRVDVEHVRTTRQLQR
                     WVGIGSATARALPPKR"
     gene            complement(5894..7948)
                     /locus_tag="PKY13_05015"
     CDS             complement(5894..7948)
                     /locus_tag="PKY13_05015"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOT79322.1"
                     /translation="MAPPTENSTQAYDEHSIQLIEGLDAVRKRPGMYIGGTGSEGLHH
                     LVWEIVDNAVDEAAAGFCNLIEVTLHKDRSVEVSDNGRGIPTGKKDAKRTALEYVFTE
                     LHAGGKFGSGAYSASGGLHGVGASVVNALATKLIVEVDREGQTHRLCFNERKPGRFGP
                     NGGFKATSELEIVKKVPPKRTGTRVRFWPDNEIFDPDAVIEYSHVRDHVAQVCFLVPG
                     LKVRLIDKRGSEPREPEEFVAKGGLSDYVDFLSIGEPVSEIVTIFGSGSFTEKVPVDG
                     KMTDVTRSCQVDVALRWVKGYDTTLVSFVNTIPTREGGTHVAGFERAMTKVVNDVLLS
                     TTKKLAKLAKQGKDRAEKTDVQEGMVAAVKVTFAEPQFKGQTKGELGTPAVQNIVYEA
                     VKDGLTNWIEGGGRKTHVVAVRDKIAQAVINRVSTKQNLDAKRKAANLGSTGMPDKLA
                     DCRTHGMGSELLLVEGDSAAGPAKAGRNSEFMAVLPLRGKVVNAGKSNLKQVIENAEA
                     QALITAIGAGSGRDFNLADARYGRIIILCDADVDGSHIRCLLLTLIYHYMRPMLEDGR
                     VFAAQPPLFTTKIGDQKYHAYSDEDKERLTVELCTGNRKRENLTWVRFKGLGEMDVEE
                     LAESCLDPETRILRRITMEDALLDTKAGEMFETLMGADVARRRQFLLDNSSLVDPEML
                     DV"
     gene            complement(7986..9236)
                     /locus_tag="PKY13_05020"
     CDS             complement(7986..9236)
                     /locus_tag="PKY13_05020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT79323.1"
                     /translation="MAVKTVAYLSGGYPRVTDAFITREIAGVEACGITVERFAVRAPD
                     DDKMTGPEQRRERDLTTYLLGGSKAELATAVVRAAIRSPKRFVSAAKLALHTRRVGVE
                     GSVRHVAYFVEAAVLAGHLRARKVDHLHNHFSDSSCTVAMLASELSGIPYSFTIHGPA
                     IFFEPREWSLETKMNTVAFCAAISAFAKAQASIYCDPSRWDLIHVVHCGVDDLRPAAD
                     ERDPERLELLFVGRLEPVKGIAVLLESVGKLVAAGRNVRLTVAGDGALREDLERRAAS
                     LGDAVRFVGYRSQPEIRQHLAESDALVLPSFAEGVPVSLMEAMSQERAVVATSVGGVT
                     ELVKHGVNGLVVHPGDPIALADALTTLADDPELRRTMGLAGRETVRADFDSATEARRL
                     VALFRDPEGTRGMVRPDPVAPESA"
     gene            complement(9267..11747)
                     /locus_tag="PKY13_05025"
     CDS             complement(9267..11747)
                     /locus_tag="PKY13_05025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HOT79324.1"
                     /translation="MPSQGDLFETNFAREVIEIPVQQEMSESFLAYSLSVITSRAIPD
                     VRDGLKPVQRRILYSMSSMGLRPDRPHRKCAGVVGDTMGKYHPHGDGSIYDALTRMGQ
                     TFSRNVCLVDPHGNFGTLDDPPAAYRYTECRLSEAAMELLAEIDEETVEFRATFDGER
                     EEPIYLPARLPNLLVNGTTGIAVGMATNMPPHNLVEVYEAIKLVMTKRRPKPTVEELR
                     AVLPGPDLPSGGMIIDDSIDEAYATGRGTFRMRATAEIVNITKTRQGIVVTELPYMVG
                     PERIVSRIQELVNTGKLVGVSDVKNLQDRRTGMNITIECKPGVNAKAILTELYRLTPM
                     EESYGVNNVVLVDGVPTTVGIWDLCNYYAEHRLDVVRRRTTYRLGRARDRLHILEGLL
                     IALDAIDLVISIIRSSQDAAEARTRLMEQLSLSDIQASHILDMQLRRLTALEKLKLEQ
                     EVAELRDLIVDYEKILDSEQRQRTIVLKELEDMVKRFGSPRRTRIIRPDQIDDVSLEA
                     MLDDPEGMTDDPCAVTLTASGLVGREPAEGPKQATFGRHDVLRARVHTTTMSKVMAIT
                     SAGRALVSPAAAISEVGGRSRGVAVAEAFATQKGESALTLVAAPTAPATAPEGELLAE
                     TAAPTALPPIMLVTANGVVKRLTNDEVMTTANTKPVIGLKPGDTVVAAFEAPDDAEVV
                     IVTSNAQALRIDASTVSVQGRGAGGVAGIKLADGATVVAAGPAGPDAVVLTHTDQQTA
                     KVTDAAEVPLKGRGTGGVRITKFRDERALDFAYVGPESGLSVVVGTEEAPSKPDANPE
                     PLTIAHTARDLVSRPVPRRILDVGFGRW"
     gene            11937..12650
                     /locus_tag="PKY13_05030"
     CDS             11937..12650
                     /locus_tag="PKY13_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79325.1"
                     /translation="MSGYDLPMLSTVADVATLVSTRAAFHTLAERVLAPDLQRATGRI
                     GLRRTAGGFGQPEHVVDGVRRRIRVDGMFLVVDHDGDGESWHDITTLGAAAALVGLPP
                     DADTGVFVATSAGDPDAPLAVDPVAAATIAEWFRFVDTALEEFRHRHAALLPSVVQLW
                     PEHFDLACSIGEINFGGSPGDAGDSGRVEPYLYVGPWSPPPVGGFWNEPYGAALERSA
                     VTTVDDALEFFERGLAAAS"
     gene            12665..>13835
                     /gene="egtB"
                     /locus_tag="PKY13_05035"
     CDS             12665..>13835
                     /gene="egtB"
                     /locus_tag="PKY13_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HOT79326.1"
                     /translation="MPSTQSDIRSDLAEEYRRVRSTTEWLAEPLGAEDQTVQSMPDVS
                     PTKWHRAHTTWFFETFVLGHHQPGYRVYDSGFDYLFNSYYEAVGPRHPRAQRGLISRP
                     GVGEVTAYRQVVDDAMVALLDGGVSDEVAALVELGLHHEQQHQELLLMDIKHVLSCNP
                     TRVAYRPPASPPSGADPATLPVSWLEFDGGLVEVGHDDSGFAYDNEGPRHRVWLEPYR
                     LADRLVTCGEWLEFMADGGYERPELWLSDGWATVKAQGWTAPLYWRRGDLGNADASTP
                     WSRFGLMGPRPVSHAEPVCHVSHYEADAFATWSGARLPTEFEWEHAVVASGSAAGEPP
                     RIGWQPSPTAGRSDGSVRQWRGDVWQWTASPYIAYPGFRPAPGAVGEYNGKFMSNQ"
BASE COUNT         2105 a         4542 c         4722 g         2266 t
ORIGIN      
        1 tctccagaac ctggcgtgga tctcgttcgt gaccggccgc atcgtcgatg ccgaatcacg
       61 cctcggcgag gccttcgaag cgttcaccga gatcgacgac cagaccgggt tggcctggac
      121 cgagggtctc ctggcgttcg tgcggttcta ccaagggcgg ttcgccgagg ccggcgagct
      181 cgcaagccgg atcctgcgcg agagcgaacg gcgaaacgac cgttgggccc aggcgatgat
      241 gctcatcgtg atcggagcca tcgacctgtg ggagggccga accggcgccg cgctcgaggt
      301 ggccaaccga tcggtctcgc tcttcacctc gatcggcgat ccgacggggc tccagcaggc
      361 gctgacgctg tcgggccgtg ggcatctcat gtcggggtac atcaccgaag ggctcgagat
      421 ggtcgaccgc gccgtcggcg acctgacgat cgacacgatc ggcccgcaga gcatgatgtc
      481 ggtcgcgtcg tcgatcgcag ctcgggtgca gctcggtgtc gagcaggcgt tcgacccgct
      541 gaccgtcgcc aagttgatcc agaacgatgc caccggcgaa cgccccgccg agttcgtcgc
      601 cgccgtcagc ctcgccctcg tgcaggccgg tcacctcgat gcggcggtcg aactgctcgc
      661 agtcgaccac gacgcccaac gcttcgccga ccgcaagggc agtgtgctga acggatacgc
      721 cctgtcgagt cgggcgctca tcgccgcgat ccagggcgat cacgaagcgg tggcagcgat
      781 cgacgcgcag gttggtgacg acagcaccta cctcgaccgg gcgatggtca agatcgcgct
      841 cgggctcggt cgggcgtcga gctggatcga cgacttcgcc cacgcccgcg ccgaggtcga
      901 cggcacgggg gatcgtctca cccgcgcgac gatcgatctg gcacgggcat cggtggcgga
      961 ggccgtgggc cacgacgccg acgtcacctg gcgcgacgag gccgaagcga gctggaccga
     1021 gctgggggtc gacccggtcg gctggcgact cgtcttctcg ttggcgaccg cgacagcgcc
     1081 cgccggggcc tgaagcggag cactgggctc cgctcctcgg ccggccgacg ggcgctggat
     1141 ggtgtgttcg atcagaccag ccttggggca gttccgatca ggcttgcggc tgttctgatc
     1201 agccttgcgg ctggggcacg tagcgcaggt acggcttctc ggcgcgccac ccctcgggga
     1261 acttctcctt cgcggcctcg tcggtgacgc cgctgccgat gatgacctcg tcaccctcgt
     1321 tccagttggc cggggtggcc accgagaact tggcggtcag ctggagcgag tcgaccacgc
     1381 ggaggatctc gtcgaagttg cgcccggtgg aggcagggta ggtgagctgg agcttcacct
     1441 tgttgtcggg gccgacgatg tacaccgagc gcactgtcag ggtgtcattg gcgttggggt
     1501 ggatcatgtc gtagaggtcg gacaccttgc gatcaccgtc ggcgatgatc gggaagttga
     1561 cctcggcgcc ctgcgtttcg gcgatgtcct tcgcccactc ggtgtggttg ccgagctcgt
     1621 cgaccgacag gccgatgacc ttgacgttgc gcttgtcgaa ctctggcttg agcttggcga
     1681 cggcaccgag ctcggtcgtg cagaccggcg tgaagtcctt ggggtgggag aagaggatcg
     1741 cccagctgtc acccttccag tcgaggaagt tgatggttcc ctgggtggtc tctgccgtga
     1801 agtcgggggc gtcgtcgccg agtcggacgg tcatggttgc ctcacatctt tcgtatgcgt
     1861 cggattcatg gatcgaacct gcaggtccga gaatacccga ccaaattagt cgggtatctc
     1921 tggaaccggc aagggggtcg gcgcattccc tccgatccgt cacgtctgcc gaggcgtgac
     1981 ggacgacacg caaacaattg caagcaaaag ggttgacgga gtgagcatct gcttgctaca
     2041 gttagcacat accaacggag cctggtcgcc aagagagaca gggctcactt ccaaaaggaa
     2101 acccctcatg actacccccg tgctcgacaa gctcaccgac gtgcagtcct cggtcgtcgg
     2161 atacatctcg actgtcaagg agcccatcac caccggtgtc tccaccgtcg tcgagttcgt
     2221 catcgaccgg ttcccgagca tcccggccgt gccctacgcc gaccagatcc ccacccccaa
     2281 ggaactgatc gacaaccagt acaagttcgc caagtcgatc atcgacacga acaaggacat
     2341 cgccctcgcg gtcgccaagg ccgctgcgcc cctgaccgac gtcgtgctcg accgcaagcc
     2401 ggtcgccaag aaggtcgccg ccaagaagac ggcgtgatct gatcccgtcg gtgccggctc
     2461 cggtcggcac ccgggccaac gtgcttcgac ctcggtcgag ccagatccct ggcgggatcc
     2521 acagatgtct cgggcggcta ccccccttgc cgccgagatg ggtcgacggc gaaccgccgg
     2581 acgaccgacg agggccctac cccccggggc cctcgtctct ttttcggatc gaggccacct
     2641 ccgctaggca cggtcgcttc gctccctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnncac gtggtcgagg agattcggca ggttgatcgc cgtccacgtg aacagcgccg
     2821 actcccgaag ccgctcggcg tccatccccg cgaacaggtc ccagaagccg ccgggctcgt
     2881 cgttcgcccg caacgcgtgc tgcacgaacc ggtgcacgta ccacccctcg atgtcggtca
     2941 cgtcggcatc cagatagacc gacaggtcga gcagttcgac gatctccgga gcgccgagca
     3001 acgccaaccc ctcgatcacc acgacgtccg cagtgggcaa ggcgtgggtc gcgccgtcga
     3061 cgatgtcgta ggtgaagtgg tcatacaccg ggacgaccag atcgttcgct ccggccctca
     3121 ccctccgaag gaacgacccc agcgcgtcga ggtcgaacga ggccggatac cccttctgca
     3181 tcaccagccc tcgatcggcg agcacggcgt tgggaagcag gaaactgtcg ctcgacacga
     3241 ccgcggtcga caccgtcggc gcaccgccag tgatcgtctc ggcgagacga tcggccaggg
     3301 tgctcttgcc gacagcgaca ctgccggtga tgccgacgac gaacggacga tcgccgactc
     3361 gtcgatcctc gatcagtcgt gcgagctctg ccagggccac cgggctgtcc accgcgggca
     3421 acctacggcg atcagcgggc cgacggccag atcagtcgag cttcgaccaa cggttcgcga
     3481 tctcctcgcc gatcgcgagg ctcgcagtcg ccgccggtga cggggcgttg acgacgtgca
     3541 ccgagcgagg cccatcggcg aactcgaagt cgtcgacgag cgtgccgtcg ggtcggatcg
     3601 cctgcgcccg gatgccggcc ttgctcttga tcagatcgtt ggcagtcacc gccgggacga
     3661 ggttctggag cgcccggacg aacgcggcct tcgacaacga gcgcaccatc tcaccggcac
     3721 cggtccgcca gtacttcttg gcgaggatcc aactgccctt gtcgaacgcc atctccttga
     3781 gatcgatgcg gttggcgacg cgccacgtgt agccctcgcg ggccagcgct accaccgcgt
     3841 tggggccggc gtggatctcc ccgtcgatca ttcgggtgaa gtgcacgccg aggaacggga
     3901 atcgtgggtc gggcaccgga tacacgaggt gcttcacgag cgaccgggcg ctcggcgtga
     3961 gctcgtagta ctcgccgcgg aacggcatga tgcgcgcctt cacctgccga cccgccagct
     4021 tcgccacccg gtcggaatgg agacccgagc agttgatcat cgtgcgggcg cgcagcgcac
     4081 ccttcgacgt acgcagtgtc accccatcgg tggcctcggt gatcgattcc accggtgtgt
     4141 cgaagtggac ctcgccgcca ttggcttcga tctcggtgac cagcgcccga cacatcgcca
     4201 cgtagtcggt gattccggcc tcgggcacga gcagcgcacc caggccctcg acattgggtt
     4261 cgcgctcgcg cagctcggcg cgggtcagcc acgtcgtgtt caacccgtgc tgctttcccc
     4321 gggcctccag gccgcggagc cgctccaact cgtcgacgcg ggttgcgacg atgaccttgc
     4381 cgcacacatc ggtggggatg tcatgacgct cgcagaagtc gaacaacatc gaacggccgg
     4441 cggcaaccat gttcgccttg ttcgaacccg gtgggtagta gatgccggaa tggatgacac
     4501 cggagttgcg gcccgtctgg tgcgcggcga ccttgggctc cttctcgacg accgtgaccc
     4561 gggtaccggg gcggttggtc tgcactgctc gcgcggcggc caggccgacg attccggcac
     4621 cgacgacgac gacgtcgaga tcgggggttg aaggggcgtt gttgctcatg atgctccgaa
     4681 gaagtcgagc acgacgttcg tggcggagaa cgagtgatcg gtgggtcctg tcacgatatc
     4741 gagcgggatc ggagaacccg cccaggtgtg accggcgccc aacatccggt agtactcgag
     4801 gcggtgagcg ccacagctgt accggtcgat cgtgaccgag gaggtcaggg tgaccgcggc
     4861 cggcggaccg acgcacccgt tgcgcccggc gaaactcgcc acgacggcgt cgacggtgag
     4921 accgagtggc ggagcgaaga ccgtctcgct tcccgtcacc ggggcgatcg gatcagcgga
     4981 cccgtgcaga atcatcgtgt cgaccggtgc gccgtcgggg cacagcgacg tgaggttcgt
     5041 gccgccggag ccggcgatcc cccggaagcg gccgggaagc tcgcagctga cggccaccga
     5101 catcgccgca ccggccgaga acccggcagc gaagacgcgg tcgccgtcga cgcagaccgt
     5161 gtcggcggtg gtccggatga gggcgtcgac ccaggcgatg tcgtcgggcc cggtgtcgag
     5221 cccacccggg accgcccagc gaggacccgg gtccgacccc tgcggcagga gcacccagta
     5281 accccgctgg gacgccgcgg cggcgagacc gctgtacgcc tcccacgcat cgggggcgag
     5341 gatgaacgga tggaggctca cgagcagcgg gtacgccacc ccgggctcgt agtcggccgg
     5401 cgcgatcagg gccgacccac ggccgagcgc ggtgatgctc tgcggtgcca ccgtcggtgc
     5461 gttcgccgga cactcggtgg ggagtggggt cacggtgcga accgtggtcg ctcgggtggg
     5521 aatctccggc gcacaggcgc tcaccagcgc gacgacggcc accatcaggc tgccgatcac
     5581 tcgacgatgc atgtcatctc ccaactcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnngtc gatccggccg attccgaacg cctccagaag ggcgccggtg tcgcggccat
     5761 ggagatgcgg ggccgctcga tgaccggttg gctccgagtg gacgtcgagc acgtgcggac
     5821 gacgcgtcag ctgcaacgct gggtcgggat cggttcggcc accgcacggg cgctgccccc
     5881 gaagcgctga gcctcagacg tcgagcatct cgggatcgac caggctcgag ttgtcgagca
     5941 ggaactgacg gcgacgggcg acgtcggcac ccatgagggt ctcgaacatc tccccggcct
     6001 tcgtgtcgag cagcgcgtcc tccatggtga tgcgacgaag gatgcgggtc tcggggtcga
     6061 ggcacgactc ggccaactcc tcgacgtcca tctcgccgag gcccttgaac cggacccacg
     6121 tgaggttctc gcgcttgcgg ttgccggtgc acagctcgac ggtgagacgc tccttgtcct
     6181 cgtcggagta cgcgtggtac ttctggtcgc cgatcttggt ggtgaacagc gggggctgcg
     6241 ctgcgaacac ccggccgtcc tcgagcatcg gccgcatgta gtggtagatc agcgtgagca
     6301 acaggcagcg gatgtggctg ccgtcgacgt cggcgtcgca gaggatgatg atgcgcccgt
     6361 atcgggcgtc ggccaggttg aagtcgcggc ccgacccggc gccgatggcc gtgatgaggg
     6421 cctgcgcctc ggcgttctcg atgacctgct tgaggttgga cttgccggca ttgacgacct
     6481 tgccgcgtag cggcagcact gccatgaact cggagttgcg tccagctttg gccgggcccg
     6541 cggcggagtc gccctcgacg agcaggagct cggagcccat gccgtgcgtg cggcagtcgg
     6601 ccagcttgtc gggcatgccg gtcgagccga ggttggcggc cttccgcttg gcatcgaggt
     6661 tctgtttcgt cgacacccgg ttgatgacgg cctgggcgat cttgtcgcgg acggccacca
     6721 cgtgggtctt gcggccgccg ccctcgatcc agttcgtgag cccgtccttg acggcctcgt
     6781 acacgatgtt ctgcacggcc ggcgtgccca gctcgccctt ggtctggccc ttgaactggg
     6841 gttcggcgaa cgtgaccttg accgcggcaa ccatgccttc ctggacgtcg gtcttctcgg
     6901 cgcggtcctt gccctgcttc gcgagcttcg ccagcttctt cgtcgtcgac aacaggacgt
     6961 cgttgacgac cttggtcatc gcccgttcga acccggcgac gtgagtgccg ccctcacggg
     7021 tggggatggt gttgacgaaa ctgaccaggg tcgtgtcgta ccccttgacc caacggaggg
     7081 ccacgtcgac ctgacaggag cgggtgacgt cggtcatctt gccgtcgacc ggcaccttct
     7141 cggtgaaact gcccgacccg aagatcgtca cgatctcgct caccggttca ccgatcgaca
     7201 ggaagtcgac gtagtcgctc agcccgccct tggcgacgaa ctcctcgggc tcacggggct
     7261 cactgccgcg cttgtcgatg aggcgcacct tgaggccggg gacgaggaag cacacctgcg
     7321 ccacatggtc gcgaacgtgg ctgtactcga ttacggcgtc gggatcgaag atctcgttgt
     7381 cgggccagaa gcgcacgcgg gtgcccgtcc gcttcggcgg caccttcttg acgatctcga
     7441 gctcggacgt cgccttgaac ccgccgttgg ggccgaaccg gcccggcttg cgctcgttga
     7501 agcagaggcg gtgcgtctga ccctcgcggt cgacctcgac gatcaacttg gtggccagag
     7561 cgttcaccac cgatgcgccc acaccgtgga gaccgccgga cgccgaatag gcaccggagc
     7621 cgaacttgcc gccggcgtgc agctcggtga agacgtactc gagcgcggtg cgcttggcgt
     7681 ccttcttgcc ggtggggatg ccgcggccgt tgtcgctcac ctcgaccgaa cggtccttgt
     7741 ggagcgtgac ctcgatgagg ttgcagaacc cggcggcggc ctcgtcgacg gcgttgtcga
     7801 cgatctccca caccaggtgg tgcaggccct ccgagccggt gccgccgatg tacatgcccg
     7861 gccgcttacg caccgcatcg agcccctcga tgagctggat cgagtgttcg tcgtaggcct
     7921 gggtggagtt ctcggtcggg ggcgccatgc ggggccaggc tagtgatccc cactgacagc
     7981 cagagtcatg cggattcagg ggcgacgggg tcggggcgca ccatgccccg ggtgccctcg
     8041 gggtcgcgga acagcgcgac caggcggcgg gcctcggtcg ccgagtcgaa gtcggcccgt
     8101 accgtctctc ggccggcgag gcccatcgtg cggcggagct cggggtcgtc ggcaagggtc
     8161 gtcaacgcgt cggcgagcgc aatcgggtcg ccggggtgca ccacgagacc gttgaccccg
     8221 tgcttgacca actccgtgac gccacccacg ctcgttgcga cgacggctcg ctcctgcgac
     8281 atcgcctcca tcagcgaaac gggcacgccc tcggcgaagc tcggcagcac cagggcgtcg
     8341 gactcggcga ggtgctggcg gatctcgggc tgggagcgat agcccacgaa ccggacggcg
     8401 tcgcccaagg atgcggcgcg acgctccagg tcctcgcgca aggccccgtc gccggccacg
     8461 gtcagccgca cgttgcgccc ggcggccacc agcttgccga ccgactccag cagcacggcg
     8521 atgcccttca cgggttccag ccggccgacg aacaggagct cgaggcgctc gggatctcgt
     8581 tcgtcggcgg ccggtcggag gtcgtcgacc ccgcagtgca cgacgtggat gagatcccac
     8641 cgcgacggat cgcagtagat gctcgcctgg gccttggcga acgcgctgat cgcggcgcag
     8701 aaggcgaccg tgttcatctt ggtttcgagg ctccactccc ggggctcgaa gaagatcgcc
     8761 ggaccgtgga tcgtgaagct gtacgggatg ccggagagct ccgacgccag catggcgacc
     8821 gtgcagctcg agtcggagaa gtggttgtgc aaatggtcga ccttgcgagc gcggagatga
     8881 ccggcgagca ccgccgcctc cacgaagtac gcgacgtggc gcaccgaccc ctcgacaccg
     8941 acccgtcggg tgtgcagcgc gagcttcgct gcggacacga accgcttcgg ggatcggatc
     9001 gcggcccgca ccaccgccgt cgccagttcg gccttcgagc cgcccagcag gtaggtcgtg
     9061 aggtcgcgtt ctcgccgctg ttcgggcccc gtcatcttgt cgtcgtcggg cgcccgcacc
     9121 gcgaagcgct cgaccgtgat gccgcaggcc tcgaccccgg cgatctcccg tgtgatgaac
     9181 gcgtcggtca cccgggggta accgccgctc aggtacgcga ccgtcttcac tgccatgagg
     9241 ccagagtgtg ccagatcagg gcacgatcac cagcggccga agcccacgtc caggatgcgt
     9301 cggggaacgg ggcgactgac gaggtcgcgg gcggtgtggg cgatcgtgag cggctcgggg
     9361 ttggcgtcgg gtttggacgg agcctcctcg gtgccgacga ccacgctcag ccccgactcg
     9421 gggcccacgt acgcgaagtc gagcgcacgc tcgtcgcgga acttggtgat gcgaacacca
     9481 ccggtacccc ggcccttgag cggcacctcg gcggcatcgg tgaccttggc cgtctgctgg
     9541 tcggtgtggg tgagcacgac ggcatcgggt ccggccgggc cggcggccac gacggtggcg
     9601 ccgtcggcga gcttgatgcc cgccacacct ccggcgcctc ggccctgcac cgacaccgtc
     9661 gacgcgtcga tgcggagcgc ctgggcgttg gacgtgacga tcacgacctc ggcgtcgtcg
     9721 ggcgcctcga aggcggccac cacggtgtcg cccggcttca ggccgatgac cggcttggtg
     9781 ttcgcggtgg tcatgacctc gtcgttggtg agtcgcttca cgacgccgtt ggcggtgacc
     9841 agcatgatcg ggggcagcgc ggtcggtgcc gccgtttcgg caaggagctc tccctcggga
     9901 gcggtggcgg gtgccgtcgg agctgcgacc aacgtgagcg ccgactcgcc cttctgggtc
     9961 gcgaacgcct cggcgaccgc gacccctcgc gagcgaccgc cgacctcgga gatcgcggcg
    10021 gcgggcgaga cgagcgctcg accggcgctg gtgatcgcca tcaccttgct catggtggtc
    10081 gtgtggactc gcgcccgcag cacgtcgtgg cgcccgaacg tcgcctgctt cggaccctcc
    10141 gcgggctcac gaccgaccag gcccgacgcg gtgagtgtca cggcgcacgg gtcgtcggtc
    10201 atgccctcgg ggtcgtcgag catggcttcg agcgacacgt cgtcgatctg gtcggggcga
    10261 atgatgcggg tgcggcgagg tgaaccgaac cgcttcacca tgtcctcgag ttccttgagg
    10321 acgatggtgc gctgacgctg ctcggagtcg aggatcttct cgtagtcgac gatgaggtcg
    10381 cgcagctcgg cgacctcctg ctcgagcttc agcttctcca gcgcggtgag gcggcgcagc
    10441 tgcatgtcga ggatgtggct cgcctggatg tcgctcagcg acagctgttc catgagtcgg
    10501 gtgcgagcct cggcggcgtc ctgcgaggag cggatgatcg agatgacgag gtcgatcgcg
    10561 tcgagggcga tcaggaggcc ttcgagaatg tgcaggcggt cgcgggcgcg gccgaggcgg
    10621 taggtggtgc ggcggcgcac cacgtcgagg cgatgctcgg cgtagtagtt gcagaggtcc
    10681 cagatgccga ccgtggtggg aacgccgtcg acgaggacga cgttgttgac gccgtaggac
    10741 tcctccatcg gagtgaggcg gtacaactcg gtgaggatcg ccttggcgtt gacgcccggt
    10801 ttgcactcga tcgtgatgtt catgccggtg cggcggtcct gcaggttctt cacgtcggag
    10861 acgccgacga gcttgccggt gttcaccaac tcctggatac gcgagacgat gcgctcgggc
    10921 ccgaccatgt agggcagctc ggtcacgacg atgccctggc gcgtcttggt gatgttcacg
    10981 atctcggcag tcgcccgcat ccggaacgtg ccgcgaccgg tcgcgtaggc ctcgtcgatc
    11041 gagtcgtcga tgatcatgcc ccccgacggc aggtcggggc ccgggagcac ggcgcggagt
    11101 tcctcgacgg tgggcttcgg gcggcgcttg gtcatcacca gcttgatggc ctcgtagacc
    11161 tcgacgaggt tgtggggcgg catgttggtg gccatgccga ccgcgatgcc ggtcgtgccg
    11221 ttgacgagca ggttcggtag gcgcgccggc aggtagatcg gctcctcgcg ctcgccgtcg
    11281 aacgtggcgc ggaactcgac ggtctcctcg tcgatctccg ccaacagctc catcgcggcc
    11341 tctgacaagc ggcactcggt gtagcggtag gcggcgggcg ggtcgtcgag cgtgccgaag
    11401 ttgccgtggg gatcgacgag gcacacgttg cgcgagaacg tctggcccat gcgggtgagc
    11461 gcgtcgtaga tggagccgtc gccgtggggg tggtacttgc ccatcgtgtc gccgacgacg
    11521 ccggcgcact tgcggtgggg gcggtcgggc cgaaggccca tggacgacat ggaatagagg
    11581 atccggcgtt gcacgggctt gaggccgtcg cggacgtcgg ggatggctcg cgacgtgatg
    11641 accgacaggg aataggcgag gaatgactca ctcatctcct gctgtacggg gatctcgatg
    11701 acctcgcgtg cgaagttggt ctcgaacagg tcgccttgtg agggcatggg gcactccagc
    11761 tgcaatcggg ggtcgcggac cggcagcgac ggactgctgg aacgcgtgtt cggcggattc
    11821 taggttggcc ctccgacagc ctcggtgacg gtggggacgg ggccgtcccg gtacccgggg
    11881 gaactgttct ccgtcctgta aggggcaatg tcaggggttc atgtaaggct ctcgtcatgt
    11941 ccgggtacga cctccccatg ctctcgacgg tggccgatgt cgccacgctc gtctccaccc
    12001 gcgccgcgtt ccacaccttg gccgagcggg tgctcgcgcc cgatctgcag cgcgccacgg
    12061 gccgcatcgg tctccgtcgg accgccggcg gattcggcca gcccgaacac gtcgtcgacg
    12121 gggttcggcg ccgcatccgt gtcgacggca tgttcctcgt ggtcgaccac gatggggatg
    12181 gcgagtcctg gcacgacatc accacgttgg gggcggccgc agccctggtc ggcctgccgc
    12241 ccgacgccga taccggcgtc ttcgtcgcca cctccgccgg cgaccccgat gctccgctcg
    12301 ccgtcgatcc agtcgcggcg gcgacgatcg ccgagtggtt ccggttcgtc gacaccgcgc
    12361 tcgaggagtt ccgccaccga cacgccgccc tccttccgtc ggtggtgcag ctgtggcccg
    12421 agcacttcga cctcgcgtgc tcgatcggcg agatcaactt cggcggctcc cccggcgacg
    12481 ccggcgactc gggtcgggtg gagccctacc tgtacgtcgg cccctggtcg ccgccccccg
    12541 tcggcggctt ctggaacgag ccgtacggcg cagcactcga acggtccgcc gtcaccacgg
    12601 tcgacgacgc gctcgagttc ttcgaacgag gcctcgcggc agcatcctga acaggggaga
    12661 ttccatgccg agcacacaga gcgacatccg gtccgacctc gccgaggagt accgccgagt
    12721 gcgctccacc accgagtggt tggccgagcc cttgggcgcc gaggaccaga cggtgcagtc
    12781 catgcccgac gtcagtccca ccaagtggca tcgtgcccac acgacgtggt tcttcgagac
    12841 cttcgtcctc gggcaccatc aacccggcta ccgggtctac gacagcgggt tcgactacct
    12901 gttcaactcc tactacgaag ccgtcgggcc gcggcacccg cgggctcaac ggggtctcat
    12961 cagccgaccc ggcgtcggcg aggtcacggc ctaccgccag gtcgtcgacg acgcgatggt
    13021 ggcgctgctc gacggcggtg tgtccgacga ggttgccgcc ctggtcgagc tgggcctcca
    13081 ccacgagcag cagcaccagg agctgctgct catggacatc aagcacgtgc tgtcgtgcaa
    13141 cccgacccgc gtggcctacc gacctccggc gtcgccaccg agcggcgccg atcccgcgac
    13201 gctcccggtc tcgtggctcg agttcgacgg cgggctggtc gaggtcggac atgacgactc
    13261 ggggttcgcg tacgacaacg agggcccccg gcatcgcgtg tggctcgagc cgtaccgttt
    13321 ggccgaccga ctcgtcacgt gcggcgagtg gctcgagttc atggccgacg gcggttatga
    13381 gcggcccgag ttgtggctgt ccgacggctg ggccaccgtc aaggcgcagg gctggacggc
    13441 gcccctctac tggcgtcgcg gcgacctcgg aaacgcggat gcgtccacgc cgtggtctcg
    13501 attcggcctc atgggccctc gaccggtgag tcacgccgag cccgtgtgtc atgtgagtca
    13561 ctacgaggcc gatgcgttcg cgacctggtc cggtgcccgt ctgccgaccg agttcgagtg
    13621 ggaacacgcg gtcgtcgcca gcggctcggc ggcgggagag ccacctcgca tcggttggca
    13681 gccgtccccg acggccggcc ggagcgacgg ttcggtgcgg cagtggcgcg gcgacgtgtg
    13741 gcagtggacc gccagcccct acatcgccta tcccggcttt cgacccgccc cgggcgccgt
    13801 cggcgagtac aacggcaagt tcatgtcgaa ccaga
//
LOCUS       DAOSOA010000049        13701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4507_length_13701_cov_6.258904, whole genome shotgun sequence.
ACCESSION   DAOSOA010000049 DAOSOA010000000
VERSION     DAOSOA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4507_length_13701_cov_6.258904"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PKY13_05040"
     CDS             <1..597
                     /locus_tag="PKY13_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79327.1"
                     /translation="SAYGGGGGGGGAGGNGGAAGSVAGTSGGGSFGIYAANASINVSS
                     SVVTSSAGGNGGAGAAGGRGGNGGTGGNGGNKSCCEAGGGGGGGGGGAGGGGGGAGGG
                     AGGPSIAIFHVGVGSYTPTANTLFRPVTPAQGGTGGAFSAAASGGVGGVRGNCALIGG
                     CGGGADGGNANPGNNGPAGATGPNGGLFRSWDNGTTIS"
     gene            743..2011
                     /locus_tag="PKY13_05045"
     CDS             743..2011
                     /locus_tag="PKY13_05045"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HOT79328.1"
                     /translation="MIGSLVDVVFRPWRRSATWRELSHLALDVVVGGVMFAISFPLAV
                     VSVSLIVVLPGAIVASWLLFLAAHALAGVERTRFSALLDVELIDAQPVLVGATAWQRY
                     KERLGSASRWKEVAYVLVHYPLGAITTSLAVGAWCVSATLLAMPAIVAGLTDGTVRIA
                     GITIGSGGAWLLALVGLLGLLVAAPWTTVALAELDRIIGRALLGRSERKEFEERVSDL
                     QSSRTRAVDSAEAERRRIERDLHDGAQQRLIAVAMSLGIARQRLDDDPESARLLVGEA
                     HDEVKSALKELRDLVRGIHPVILEDRGLDAALSAVIARCSVPVDLRVDVVERPVAAVE
                     SAAYFIVSEALVNVVRHAQATATTVEIARRGDRLTIAVADNGIGGADPSRGTGLQGLS
                     DRVAAIDGWMQVVSPVGGPTTLMVEVPCGS"
     gene            1999..2643
                     /locus_tag="PKY13_05050"
     CDS             1999..2643
                     /locus_tag="PKY13_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT79329.1"
                     /translation="MRIVIAEDSVLLRAGLTRFLTEGGEEIVAAVGDAVALVEAVERH
                     RPDLAVVDVRMPPTHTDDGLRAALEIRRRWPAVGILVLSQYVEERYASELLAGDTGGI
                     GYLLKDRIADVGDFLDAVRRVGSGGAVLDPEVVAQILARSRRHDPIATLTPREREVLS
                     LMAEGRSNGAIAAALVVGDGAVEKHVSNIFQKLELTPAEGDHRRVLAVLRWLEA"
     gene            2654..3427
                     /locus_tag="PKY13_05055"
     CDS             2654..3427
                     /locus_tag="PKY13_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOT79330.1"
                     /translation="MNATKVRSAWVIVGSIAAVVTLGFGMVQAIGFVAREQSTVRQSF
                     PGAGLSLVEVTSDSGSIRVVGDDEESVRVVIRLEEGWSRIERVARVDGDRLVLSAACP
                     VFSGPFCSATYEVHVPRRLDVVARSENADVGASDVDGAVTAGSSNGDVTGTRLGGEAQ
                     LTTDNGSVEGVDLTSDDVVGSSSNGDVELEMARAPRSVRAESDNGDVVVIVPRGSELY
                     AVDATTDNGDAATPIRTDPSARRRIVARSDNGDVTVRYP"
     gene            complement(3442..4044)
                     /locus_tag="PKY13_05060"
     CDS             complement(3442..4044)
                     /locus_tag="PKY13_05060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT79331.1"
                     /translation="MPNRTASTLLSLLLVIVAVGCGTADEDKVGLIGDSITDLSREPL
                     TQALGEDRVVEIVGKFGARSDQVIEDVKVIAASEPAAAIINIGTNDALQQVPAAQTAA
                     NIQQILDLLGDVGCRYLVQINTGITDTATGAARQAEAAAVNDQLAQLAELNDGVEVLD
                     WNRTIADNGGNPAITYDTVHLSEKGVVLMADAYRQALDDC"
     gene            4153..5124
                     /locus_tag="PKY13_05065"
     CDS             4153..5124
                     /locus_tag="PKY13_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT79332.1"
                     /translation="MRADRLVAILMLLQTRTQVTAAEVAAELEISERTARRDLEALGT
                     AGLPVYSRQGRNGGWQLLGRARTDLSGLTAAEARALFLVAGPSSSATPEIKAALRKLM
                     RALPESFRTDAEAAASALVIDPSAWGKRTRDRWTPPLLDDLQQAVIDGVQVHLGYATP
                     RRDPSTRLVHPLGLVAKGTNWYLVADTDAGLRTFRVDRVTAVERTVDPVVRPAGFDLA
                     STWKELVDGATERWATLRAAGFAAPSIVGRLGGLFGDRLQIGPARASDGWVEVEVSGF
                     ELRALAGELAGWGGSLELTGPPELTELLAAIGAELVATYAGSGERSG"
     gene            complement(5148..5870)
                     /locus_tag="PKY13_05070"
     CDS             complement(5148..5870)
                     /locus_tag="PKY13_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOT79333.1"
                     /translation="MLLSVLLGSDPPRLPTQLLVRTTELFGIAEGTTRTALSRMAAAR
                     EVTAHGASYELTGPALLQRQARQSTSRAGRTARWDGTWSQAVIGADGARPAPDRAALR
                     QALVTFRHAELREGLWLRPANLGPQPELEYVRWFTATPAGDPSELATRLWDLVDWSKE
                     ANDLRRRMTRLVGPLEAGDRSALADGFVLSAAVLRQFQSDPLLPDTLLPPDWPGIALR
                     SEYDRYDRAYRAVLQEWFREQT"
     gene            5945..6952
                     /locus_tag="PKY13_05075"
     CDS             5945..6952
                     /locus_tag="PKY13_05075"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOT79334.1"
                     /translation="MASPISSTVMGAPRLGGGNMVPHPDLLPAVLPTDRLTVSAMPVP
                     ELRVELRRIDDVRNVGSVVLCWAHAALTIGLAVRLANPFAYAAAVVLMGPVFARFAIL
                     GHEAAHKLLFTNKRANDIVGKWLLASPAFVPFKLYRRSHFAHHKEEFGPNEPDLSFYV
                     GYPVSRRSLRRKLVRDTVGISGWKNLKVLLKALGKSSSRPFAAPIAYAQVAVFAVFLL
                     IGRPELYLLLWLLPWMTSWRVVNRLRAIAEHGGMEASRDRRRTTHHVHQSLPAKFWIV
                     PFNTGWHLAHHVDMGVPWRNLPKLHAELVAAGWVVPELEYRNYRTLWRAARSGEERVR
                     A"
     gene            6949..7746
                     /locus_tag="PKY13_05080"
     CDS             6949..7746
                     /locus_tag="PKY13_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOT79335.1"
                     /translation="MSEVRVETDGPIMIVTIDRPDVRNAVNRPAADRLVDAFGSFDAD
                     DSLSVAILSGAGGTFCSGADLKAVASGTGNRIEADIDLAGPMGPTRMDLSKPVIAAIE
                     GHAVAGGLELAVWCDLRVAASDATLGVYCRRWGVPLVDGGTVRLPRLVGHGRAMDLIL
                     TGRGVGADEAHRIGLVDRVVAPGTALAAAVDLAHQLAALPQTCLRSDRRSAIDQWSLD
                     SRDALAHETDLGLATVRSGETREGAARFAAGEGRHGAAGEGRHGAGA"
     gene            7763..>8382
                     /locus_tag="PKY13_05085"
     CDS             7763..>8382
                     /locus_tag="PKY13_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79336.1"
                     /translation="MTDTVPDPEPEPAWRRATAGESRWAASIALLIAIACQMVLPVEF
                     TTHRWLVPAIELGMLVWSLLMNPNRIDRHSGALRRVNLALIGVLTLANARAAWGLVDH
                     IVGGQATNAGRLLVSGAAIWVTNMIAFALWYWEFDRGGPGRRSEGIREYPDFLFPQMQ
                     NPDLAPKDWEPSFVDYLYLSFTNATAFSPTDVLPMTQWAKLTMLAQS"
     assembly_gap    8383..8482
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8627..9040)
                     /gene="msrB"
                     /locus_tag="PKY13_05090"
     CDS             complement(8627..9040)
                     /gene="msrB"
                     /locus_tag="PKY13_05090"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOT79337.1"
                     /translation="MSDTVDPSELTEDDWKQKLTPEQYHVLRQAGTEPAWTGDLLKEK
                     RDGTFVCGACGQHLFSSDTKFESGSGWPSFYQAADDNAVELVEDRSHGMRRVEVRCSN
                     CHSHLGHLFDDGPRPTGQRFCMNSLALDFEAAEAE"
     gene            complement(9089..9823)
                     /locus_tag="PKY13_05095"
     CDS             complement(9089..9823)
                     /locus_tag="PKY13_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT79338.1"
                     /translation="MEIPTNVQARINDIRSRFDPGALGTTSTGSTTSTGTSADFASVL
                     AETLAASDTTNGITGLGDTNNLSTLTDLLSTANASRLQSSTGRTTSLSAAQSAYGSSG
                     GVATVAPIATKRAVSNLEGLYENGRLPDEALTPIGVGSHRLAAPAASAFTKLSAAAEA
                     DGVTIGVTDSYRSYDAQVDVANRKGLYSQGGLAAKPGTSDHGLGLALDLDLDTKALSW
                     MRANAGRFGFVEDTPRESWHWKFDGV"
     gene            complement(9878..11050)
                     /locus_tag="PKY13_05100"
     CDS             complement(9878..11050)
                     /locus_tag="PKY13_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT79339.1"
                     /translation="MHRRFAVPLVMVALIGVALTACSSAPGGGVPVGPTGRFTIVGRD
                     IVDPAGNRFYPIGANVSVRQGPYENGYTFNWNGTATGHAADAKAWGWNIVRANLICVP
                     PPSPTVAQLNAGIDSFIDEYTAQGIVVMVDCHDVTGANLPASSAKIQSLYPFWERLAN
                     KYKNNPYVWFNMFNEPQGGSSDGDVANWLTLQQDALRRLRAIAPNNVFVADIPGYGQA
                     VETFTGSNSILRLGAGQCNVLYSWHAYGALGKDGVFGEFADFDNEAASRDNHREALQY
                     LKDHNIPVVIGELGDPLTLDEGTAGLPIWNRIGARAVMSEAPAFGVGMLWWHATGDSG
                     NFLTYSLMADRHQAPWSAAATGAGLSNGGFRFWAVSKNAPNLGPFTGDLAASNCGS"
     gene            complement(11151..11720)
                     /locus_tag="PKY13_05105"
     CDS             complement(11151..11720)
                     /locus_tag="PKY13_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79340.1"
                     /translation="MTGAPVTATSVGEILDLYERWGPENYDEGLSQLEHALQTAALAV
                     ADGAPDEQVAAALLHDIGHLLDLAAAEDNWVPSDIDLDHESVGARHLASLFPPSVTGP
                     IALHVRAKRYRAATDPAYAATLSDGSTRSLQIQGGPMSPDEVATFERNPAFGAAVALR
                     GWDDGGKVDGLPVPRLREYRALLERVALT"
     gene            complement(11717..12541)
                     /locus_tag="PKY13_05110"
     CDS             complement(11717..12541)
                     /locus_tag="PKY13_05110"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOT79341.1"
                     /translation="MTALPRAQVLVARVFDTADAEAVEHFNTNGWLLTQTLDADGLAA
                     LRSWVDEISAWPDDGDGWLHHREMTDDGPKLCRSENLIPFHPGFRELLTTGPLLDTAS
                     ALLGEQAVLYKEKINYKLPGGAGYAPHQDAPAYPFVASHVSCMVAIDDATVDNGCLDV
                     VSGMHHDVLPMDEVGCVHPDVVAELEWAAVEVAAGQTLWFHSRTPHRSGPNTSTTARR
                     ALYPTYNARSEGDLREDYYREKLAAFAADDGSGAHTKVSLIGDFQGRAVDTGTAAG"
     gene            12600..12917
                     /locus_tag="PKY13_05115"
     CDS             12600..12917
                     /locus_tag="PKY13_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79342.1"
                     /translation="MRDGFDGITDDLGNAAGDPMADSWTDDELSELALGADPRERLTA
                     DATPLDGSVPASDGPLPGWYMPAVARVVTSPTRRIAALSVVGAFLVINALGLCATYGV
                     ISI"
     gene            complement(12985..13455)
                     /locus_tag="PKY13_05120"
     CDS             complement(12985..13455)
                     /locus_tag="PKY13_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT79343.1"
                     /translation="MLRRCAVLLIVGLVPVLSGCPAPGGGDWQAQMLRSINTIRAGAG
                     VGPVSLCGTLGAAAQQHSEDQAGRSTMSHSGADGSRIGDRAVRSGYGGWSALAENIAS
                     GQTTVDLAMWSWMSSPGHRENILDPAYSHVGFGRASSSGGTQFWTQDFGASGSC"
BASE COUNT         1837 a         4511 c         5058 g         2195 t
ORIGIN      
        1 agtgcctacg gtggcggtgg tggcggcggt ggcgccggcg gcaacggcgg cgctgcgggc
       61 tcggtggccg gcacgtcggg tggcggctcc ttcgggatct acgccgccaa cgccagcatc
      121 aacgtctcgt cgtcggtcgt gacctcctcg gccggtggca acggcggtgc cggcgcggcc
      181 ggtggccgcg gtggcaacgg tggcaccggt ggcaacggcg gcaacaagtc gtgctgcgag
      241 gccggtggcg gcggcggtgg cggcggcggt ggcgccggcg gtggcggtgg cggtgccggt
      301 ggcggtgccg gcggcccgtc gatcgcgatc ttccacgtcg gtgtcggttc gtacactccg
      361 acggccaaca cgctgttccg cccggtcacg cccgcccagg gcggcaccgg tggtgcgttc
      421 tcggcggcgg ccagtggcgg cgtcggtggt gtccggggca actgtgcgct catcggtggc
      481 tgcggtggcg gcgccgacgg cggcaacgcc aaccccggga acaacggccc tgccggtgcg
      541 accggtccca acggtgggtt gttccgttcc tgggacaacg gcaccacgat cagctgacgc
      601 tgctgcgtcg ttcgggttct cacgacatgg ggccgccctc cggggcggcc ccgttcgcgt
      661 tctcgaccgg gggacaaccc caccgtcggt ccggtggtcg acgccatggt ggtcggcgcc
      721 gccactgcct acggtgagtc ccgtgatcgg ttcgctcgtc gacgtcgtct tccgcccctg
      781 gcggcgctcg gccacctggc gagagctgtc gcacctcgcc ctcgacgtcg tcgtcggcgg
      841 cgtgatgttc gcgatctcgt tcccgttggc cgtcgtgtcg gtgagcctca tcgtggtgct
      901 gcccggcgcg atcgtcgcgt cgtggctgtt gttcctcgcg gcgcacgcgc tcgccggggt
      961 cgagcggacg cgcttctcgg ccctgctcga cgtcgagctg atcgacgcgc agccggtgtt
     1021 ggtcggagcg acggcgtggc agcggtacaa ggaacgcctc ggctcggcga gccggtggaa
     1081 ggaggtcgcg tacgttctcg tccactaccc gttgggggcg atcacgacga gcttggccgt
     1141 cggcgcgtgg tgcgtgtcgg cgacactcct cgcgatgccc gcgatcgtcg ccggactcac
     1201 cgacgggacg gtccgaatcg ccggcatcac catcggttcg ggcggtgcgt ggctgctcgc
     1261 cctcgtcgga ctcctggggc tgctcgtcgc cgccccctgg acgacggtgg ccctggccga
     1321 gctcgaccgg atcatcggtc gggcgcttct cggccggagt gagcggaagg agttcgagga
     1381 acgggtgagc gacctccagt cgagccggac ccgcgcggtg gacagcgccg aggcggagcg
     1441 gcgacgtatc gaacgggacc tgcacgacgg cgcccagcag cgcctgatcg cggtggccat
     1501 gagcctgggc atcgcccgtc agcggctcga cgacgatccc gagtccgccc gtctgctcgt
     1561 cggcgaggcc cacgacgagg tgaagtcggc gctcaaggaa ctgcgggacc tggtccgggg
     1621 gatccatccg gtgatcctcg aggaccgggg cctcgacgcc gcgttgagcg cggtgatcgc
     1681 ccggtgttcg gtgcccgtcg acctgcgggt cgacgtcgtc gaacgtcccg tcgcggcggt
     1741 cgagagtgcg gcgtacttca tcgtctccga ggcgttggtc aacgtcgtgc gccatgcgca
     1801 ggcgacggcg accaccgtcg agatcgcgcg gcgcggcgac cggctcacga tcgccgtcgc
     1861 cgacaacggc atcggtggtg ctgatccgtc caggggcact gggttgcagg gcttgtcgga
     1921 tcgggtcgcg gcgatcgatg gctggatgca ggtcgtcagt ccggtcggcg gtccgacaac
     1981 gctgatggtg gaggtgccat gcggatcgtg atcgccgagg acagcgtgtt gttgcgcgcc
     2041 ggcctgactc ggttcctcac cgagggcggc gaggagatcg tcgcggcagt gggcgacgcc
     2101 gtcgccctgg tcgaagccgt cgagcggcat cgtcccgacc tcgccgtcgt ggatgtccgg
     2161 atgcccccga ctcataccga cgacgggttg cgtgcagccc tcgagatccg ccggcgctgg
     2221 cccgccgtcg ggatcctcgt gctctcccag tacgtcgagg agcggtacgc gtccgagctg
     2281 ctggccggcg acacgggcgg catcgggtat ctgctcaagg accggatcgc ggatgtgggc
     2341 gacttcctcg acgccgtgcg ccgggtcggt tccgggggcg cggtgctgga tcccgaggtc
     2401 gtggcgcaga tcctcgcccg gtcgcggcgg cacgacccga tcgcgacgct cacgccccgc
     2461 gagcgcgagg tgctctcgct gatggcggag ggtcgcagca acggtgcgat cgccgcggcg
     2521 ttggtcgtcg gcgacggcgc ggtcgagaag cacgtgagca acatcttcca gaagctcgag
     2581 ctgacaccgg cggagggcga ccaccgtcgg gtgctcgccg tgctgcgctg gctcgaggcg
     2641 tgaggggatg ccgatgaatg cgaccaaggt ccggagcgcg tgggtgatcg tcgggagcat
     2701 cgccgcggtg gtcacgctcg ggttcggcat ggtccaggcg atcgggttcg tcgcccgtga
     2761 gcagtcgacc gttcggcagt cgttccccgg cgccggactg tcgctcgtcg aggtgacgtc
     2821 cgacagtggc agcatccggg tcgtcggcga cgacgaggag tcggtgcggg tcgtgatccg
     2881 tctggaggaa ggatggtcgc gcatcgagcg ggttgcccgc gtcgacggtg accggctggt
     2941 tctgtcggcc gcgtgtccgg tgttcagcgg gccgttctgc tcggcgacct acgaggtgca
     3001 cgtgccccgg cgcctggacg tcgtcgctcg gagcgagaac gcggacgtcg gcgcgagcga
     3061 cgtcgacggt gcggtgacgg cagggtcgag caacggtgac gtcaccggca cccgattggg
     3121 aggggaggcg cagctgacca ccgacaatgg cagcgtcgag ggcgtcgacc tgacgagcga
     3181 cgatgtcgtc ggatcctcga gcaacggtga tgtggagctg gagatggccc gggcgccgcg
     3241 ctcggtgcga gccgagagcg acaacggcga cgttgtggtg atcgtgcccc gtggctcgga
     3301 gctgtacgcg gtcgacgcga ccaccgacaa cggcgacgct gcgacgccga tccggactga
     3361 tccgtcggcg cggcggcgca tcgtcgcccg gtccgacaac ggcgacgtca cggttcggta
     3421 cccctgaccg attgcggcgc ctcagcagtc gtcgagcgcc tggcggtagg cgtcggccat
     3481 gagcacgacg cccttctcgc tcaggtgcac cgtgtcgtag gtgattgccg ggttgccccc
     3541 gttgtcggcg atcgtgcggt tccagtcgag gacctcgacg ccgtcgttga gctcggcgag
     3601 ttgggccagc tggtcgttga cggccgccgc ttcggcctgg cgcgccgcgc cggtggccgt
     3661 gtcggtgata cccgtgttga tctgcaccag gtagcgacag ccgacatcgc cgagcaggtc
     3721 gaggatctgc tggatgttcg cagccgtctg tgcggcgggt acctgctgca acgcgtcgtt
     3781 ggtgccgatg ttgatgatcg ccgctgcggg ctcggacgcc gcgatcacct tgacgtcctc
     3841 gatgacctgg tcggaacgcg ctccgaactt tccgacgatc tcgacaacac gatcctcacc
     3901 gagcgcctgg gtgagcggct cgcgggacaa gtcggtgatc gagtcgccga tcaggcccac
     3961 cttgtcctcg tcggcggtcc cgcatcccac agcgacgatg acgaggagga gggacaggag
     4021 cgtggacgcg gttcggttcg gcacaccaca tcgtgcccga ccatggggtc ccccaaaccg
     4081 ggaatccccg aaccggggga ccgaggttga accggtcatg tcttggccgg tttgtcgtgc
     4141 gaacatggcc tcatgcgcgc agatcggctc gtcgccatcc tcatgctcct gcagacccgc
     4201 acgcaggtga cggcggctga ggtcgcggcc gagctggaga tctccgagcg cactgcacgt
     4261 cgcgacctcg aggcgctcgg gacggccggt ctgccggtgt actcgcgcca ggggcgcaac
     4321 ggagggtggc aactcctcgg gcgtgcccgg accgatctca gcgggctgac cgcggccgag
     4381 gcgcgagcgc tcttcttggt ggcggggccg tcgtcgtcgg cgacccccga gatcaaggcg
     4441 gcgttgcgga agctcatgcg ggcgctgccc gagtcgttcc ggaccgatgc cgaggcggcg
     4501 gcctcggcgc tcgtcatcga cccgtcggcg tgggggaagc ggacccgtga ccgttggacg
     4561 ccgccgctgc tcgacgacct gcaacaggcg gtcatcgacg gtgtgcaagt gcacctcggg
     4621 tacgccaccc cgaggcgcga cccgagcacc cgactcgtcc atcccctcgg tctcgtggcc
     4681 aagggcacga actggtatct ggtcgcggac accgacgccg ggctccggac gttccgcgtc
     4741 gatcgggtga cggcggtgga gcgcaccgtg gatccggtgg ttcggccggc cgggttcgat
     4801 ctcgcgtcga cgtggaagga gttggtcgac ggggcgaccg agcggtgggc gacgctgcgg
     4861 gccgccgggt tcgcggcccc gtcgatcgtg gggcggctcg ggggtctgtt cggggaccgg
     4921 ctgcagatcg ggccggcccg cgccagcgac ggctgggtcg aggtcgaggt ctcgggattc
     4981 gaactgcggg cgttggccgg tgagctggcg ggatggggcg gctcgctcga gctcacgggg
     5041 ccgccggagc tgaccgagct gctggccgcc atcggcgccg agctggtcgc gacctacgcc
     5101 gggtccggtg agcgcagcgg ttgaggtggg ctcggggtgc ccggccgtca ggtctgttcg
     5161 cggaaccact cctgcagcac ggcgcggtac gcgcggtcgt agcggtcgta ctccgaccgc
     5221 aacgcgatgc cgggccagtc ggggggcagc agtgtgtcgg gcaggagggg atcggactgg
     5281 aactggcgga gtacggcggc gctgagcacg aacccgtccg cgagtgcgga acggtcgccc
     5341 gcctccagtg gaccgacgag tcgagtcatg cgacgccgga gatcgttcgc ctccttcgac
     5401 cagtcgacga ggtcccagag acgggtcgcg agttcgctcg ggtcgccggc gggtgtcgcc
     5461 gtgaaccaac gcacgtactc gagctcgggt tggggcccga ggttggcggg ccgcaaccag
     5521 agcccctccc gcagctcggc gtggcggaac gtgacaaggg cctggcgaag cgcggcccgg
     5581 tcgggcgcgg gccgggcacc gtcggcgccg atgaccgcct ggctccacgt cccgtcccat
     5641 cgggcggtcc ggccggcgcg gctcgtcgac tggcgggctt gtcgctgcag cagcgccggc
     5701 cccgtcagct cgtaggacgc cccgtgtgcc gtgacctccc gcgctgccgc catgcgggac
     5761 agcgctgtgc gcgtcgtgcc ctcggcgatg ccgaacagct cggtcgtccg caccaggagc
     5821 tgggtcggca ggcgaggcgg gtcgctgccg aggaggacgc tcaacaacac cgatcgagcc
     5881 gtcagcgcgc gttggccgtt acgacttgtt gacacgtgtt tgagtttcgt attgcatggg
     5941 gtgcatggca agtccgatct cgtcgacggt gatgggagcg ccgcgtctcg gcggcggcaa
     6001 catggtgccg caccccgacc tccttcccgc cgtgttgccc accgatcgcc tcacggtgtc
     6061 ggcaatgccg gttcccgaac tccgagtcga gctccgccgg atcgacgacg tccgcaacgt
     6121 gggcagcgtc gtgctgtgct gggcacacgc cgccctcacg atcggcctcg ccgtccggct
     6181 cgccaacccg ttcgcgtacg ccgccgccgt ggtgttgatg gggccggtgt tcgcccggtt
     6241 cgccatcctc ggtcacgagg cggcccacaa actcctcttc acgaacaagc gcgcgaacga
     6301 catcgtcggc aagtggttgc tcgcctcgcc ggcgttcgtg ccgttcaagc tgtaccggcg
     6361 cagccacttc gcccaccaca aggaggagtt cgggccgaac gaacccgacc tctcgttcta
     6421 cgtgggctac ccggtcagtc ggcggagcct gcgtcgcaag ctcgtgcgcg acaccgtcgg
     6481 catctccggc tggaagaacc tgaaggtgct gctcaaggcg ttgggcaagt cgtcgtcgcg
     6541 cccgttcgcc gcgccgatcg cgtacgccca ggtcgccgtg ttcgccgtgt tcctgctcat
     6601 cggtcggccc gagttgtatc tgctcctctg gctgctgccg tggatgacgt cgtggcgggt
     6661 cgtcaaccgg ctccgggcca tcgccgagca cggcggcatg gaagcgagcc gcgaccgtcg
     6721 gcggaccacc caccacgtgc accagtcgct cccggccaag ttctggatcg tcccgttcaa
     6781 caccgggtgg cacctggcgc atcatgtcga catgggggtg ccgtggcgca acctcccgaa
     6841 gctgcacgcc gagctcgtgg ccgcgggctg ggtcgtcccc gagctcgagt accgcaacta
     6901 ccgcacgctg tggcgcgctg cccggtccgg cgaggaacgg gtccgcgcat gagcgaggtc
     6961 cgagtcgaga ccgatggtcc gatcatgatc gtcacgatcg accgccccga cgtgcgcaac
     7021 gcggtcaacc gcccggcggc tgaccggctc gtcgacgcct tcggttcgtt cgacgccgac
     7081 gactcgttgt cggtggcgat cctgagcggt gcgggcggca cgttctgctc cggcgccgat
     7141 ctcaaggccg tggcgtcggg caccggcaac cgcatcgagg ccgacatcga cctcgccggt
     7201 ccgatgggac cgacccggat ggacctctcc aaaccggtga ttgccgccat cgagggccat
     7261 gccgtcgccg gaggactcga gttggcagtc tggtgcgatc tccgggtcgc cgcatccgac
     7321 gccacgctcg gcgtctactg ccggcgttgg ggcgtgccgc tcgtcgacgg cggtacggtt
     7381 cggcttcccc gcctggtggg ccacggccgg gcgatggacc tgatcctgac cggtcgaggc
     7441 gtcggtgccg acgaggcgca ccgcatcggc ctcgtcgacc gcgtcgtcgc tccgggcacc
     7501 gcgctcgccg cggcggtcga tcttgcgcac caactggcag cgctcccaca gacgtgtctg
     7561 cgcagcgatc gccgatccgc catcgaccag tggtcgctcg actcccgcga cgcgttggcc
     7621 cacgagaccg acctcggcct cgccacggtc cgctccggcg agacgcgtga gggtgccgcg
     7681 aggttcgccg caggcgaagg tcgacacggc gccgcaggcg aaggtcgaca cggcgcgggt
     7741 gcctgactga caacatgtcg gcgtgaccga cacggtgccc gaccccgaac ccgaaccggc
     7801 gtggcggcgg gcgactgcgg gtgagtcacg ctgggcggcg tcgatcgcac tgctgatcgc
     7861 gatcgcgtgc cagatggtcc tgccggtgga gttcaccacg caccgttggc tggtgccggc
     7921 catcgagctc ggcatgctcg tctggtcgct gctcatgaac ccgaaccgca tcgatcggca
     7981 ctcgggcgcg ttgcggcggg tgaacctggc gctgatcggc gtgttgacgc tcgcgaacgc
     8041 ccgcgccgcg tgggggctcg tcgaccacat cgtcgggggc caggcgacca acgccgggcg
     8101 tctgttggtg tcgggtgccg cgatctgggt gaccaacatg atcgcgttcg cgctgtggta
     8161 ttgggagttc gaccggggcg ggcccgggcg ccgcagcgag ggcatccggg agtacccgga
     8221 cttcctcttc ccccagatgc agaaccccga cctcgccccg aaggactggg agccctcgtt
     8281 cgtcgactac ctgtacctgt cgttcacgaa cgcgacggcg ttcagcccga ccgacgtgct
     8341 gcccatgacc cagtgggcga agctcacgat gttggcgcag tcnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nncgaccgac gtgctgccca tgacccagtg ggcgaagctc
     8521 acgatgttgg cgcagtcgtc ggtgtcgttc gtgaccgtca tccttgtcgt ggcgcgggcc
     8581 gtcaacaccc tgtcgtgagc cgacggtgcc ggtggcgacg ccggtgctac tcggcctcgg
     8641 cggcctcgaa gtcgagcgcg agcgagttca tgcagaagcg ctgaccggtc ggtcgcgggc
     8701 cgtcgtcgaa gaggtggccg aggtgggaat ggcagttgga gcagcggacc tcgacgcggc
     8761 gcatcccgtg gctgcggtcc tcgaccagtt cgacggcgtt gtcgtcggcc gcctggtaga
     8821 agctcggcca ccccgaaccg gattcgaact tggtgtccga cgagaagagg tgctggccgc
     8881 aggcgccgca cacgaacgtg ccgtcgcgct tctccttcag caggtcgccg gtccatgccg
     8941 gctcggtgcc cgcctggcgc aggacgtggt actgctcggg ggtcagcttc tgcttccagt
     9001 cgtcctcggt cagttcggag gggtccacgg tgtcgctcat ggcctcagtc tgcaccctcg
     9061 acgacgacgt ggcgacgcgc gccgggcgtt agaccccgtc gaacttccag tgccacgact
     9121 cgcgaggggt gtcctcgacg aagccgaatc ggccggcgtt ggcccgcatc cacgacagcg
     9181 ccttggtgtc gaggtcgagg tcgagcgcga ggcccagtcc gtggtcgctg gtgccgggct
     9241 tcgccgcgag tccgccctgg ctgtagaggc ccttgcggtt cgcgacgtcg acctgggcgt
     9301 cgtagctgcg gtacgagtcg gtcacgccga tcgtcacccc gtcggcctcg gcggcggcgc
     9361 tcagcttcgt gaacgccgag gcggccggcg cggcgaggcg gtgcgatccg accccgatgg
     9421 gggtcagggc ctcgtcgggg aggcgaccgt tctcgtacag gccttccagg ttggagacgg
     9481 cccgcttcgt cgcgatcggc gcgacggtgg cgactccacc cgatgagccg taggccgatt
     9541 gggccgcgct cagcgaggtg gtccggccgg tcgaggactg gaggcgcgag gcgttggcgg
     9601 tcgacagcag gtcggtcagg gtcgacaggt tgttggtgtc gccgagtccg gtgatgccgt
     9661 tggtcgtgtc gctcgcggcg agggtctcgg cgagcaccga ggcgaagtcg gccgaggtcc
     9721 ccgtcgatgt ggtcgagccg gtcgacgtgg taccgagcgc gccggggtcg aagcgcgagc
     9781 ggatgtcgtt gatccgcgct tggacgttgg tggggatctc cacggagtcc tatcggcggg
     9841 tcggggccac tgttgagcgc gcggtggtcc gttcggccta cgagccgcag ttgctcgcgg
     9901 ccaggtcgcc ggtgaacggg ccgaggttcg gcgcgttctt ggagacggcc cagaagcgga
     9961 acccgccgtt cgacaggccg gcgcccgttg ccgccgccga ccagggggcc tggtggcggt
    10021 ctgccatcag cgagtaggtg aggaagttgc cgctgtcacc ggtggcgtgc caccacagca
    10081 tcccgactcc gaacgccggc gcctcgctca tcacggcgcg ggccccgata cggttccaga
    10141 tcggcagccc ggcggtgccc tcgtcgaggg tcagggggtc gcccagctca ccgatgacga
    10201 cggggatgtt gtgatccttc aggtactgca gcgcctcgcg atggttgtcg cggctggctg
    10261 cctcgttgtc gaagtcggcg aactcgccga agaccccgtc cttgccgagc gcgccgtagg
    10321 cgtgccagga gtagaggacg ttgcactggc cggcgccgag ccggaggatc gagttcgacc
    10381 ccgtgaacgt ctcgaccgcc tgcccgtaac cggggatgtc ggcgacgaag acgttgttcg
    10441 gagcgatggc ccggaggcgg cggagcgcgt cctgctgcag ggtcagccag ttggcgacgt
    10501 cgccgtcgga gctgccgccc tgcggctcgt tgaacatgtt gaaccacacg taggggttgt
    10561 tcttgtactt gttcgccagg cgctcccaga acgggtacag cgactggatc ttcgccgacg
    10621 aggcagggag gttcgccccg gtgacgtcgt ggcagtcgac catgacgacg atgccctgcg
    10681 ccgtgtactc gtcgatgaag gagtcgatgc cggcattgag ctgggcgacg gtcggcgagg
    10741 gcggcgggac gcagatgagg ttggctcgca cgatgttcca gccccaggcc ttggcgtcgg
    10801 ccgcatgccc cgtggcggtc ccgttccagt tgaacgtgta cccgttctcg tagggccctt
    10861 ggcggaccga cacgttcgct ccgatcgggt agaaccggtt gccggcggga tcgacgatgt
    10921 cacgtccgac gatggtgaac cgtccggtcg ggccgacggg aaccccacct cccggtgccg
    10981 aggagcaggc ggtcagggcg acgccgatga gggcgaccat gacgaggggc acggcgaaac
    11041 gacgatgcat ccccagagct tcgacatctc cggcgacgag ctggagtgac gcgggtgaat
    11101 ttcggcggaa tccctcgtcg aggggacgtc gcgtcgggga ggagcgagcc tcaggtgagc
    11161 gcgacgcgct cgaggagcgc tcgatactcg cggagtcgcg gcaccggcag gccgtcgacc
    11221 ttgccgccgt cgtcccatcc ccggagggcc acggcggcac cgaacgcagg gttgcgctcg
    11281 aaggtcgcga cctcgtcggg cgacatgggc ccaccctgga tctggaggct ccgggtcgac
    11341 ccatccgaca acgtcgcggc gtacgccggg tcggtcgccg cccggtagcg cttggcgcga
    11401 acgtgcagcg cgatcggacc ggtgaccgac ggcgggaaca gcgaggccag gtgtcgggca
    11461 cccaccgact cgtggtcgag atcgatgtcg gacggcaccc agttgtcctc ggcggcagcg
    11521 aggtcgagca ggtgtccgat gtcgtggagg agcgcggcgg cgacctgttc gtcgggtgcg
    11581 ccgtcggcga ccgcgagcgc tgcggtctgc agtgcgtgtt cgagctggct caggccctcg
    11641 tcgtagttct cggggcccca gcgctcgtag aggtccagga tctcgccgac gctggtcgcc
    11701 gtcacgggag cgccggtcat ccggccgcgg ttcccgtgtc gacagctctg ccctggaagt
    11761 cgccgatcag ggagaccttg gtgtgcgcgc cgctgccgtc gtcggcagcg aacgcagcga
    11821 gcttctcccg gtagtagtcc tcacgcagat cgccctcgga gcgggcgttg taggtcgggt
    11881 acagggcgcg gcgtgccgtc gtcgacgtgt tggggccgct gcggtgcggg gtacgggagt
    11941 ggaaccagag ggtctgaccg gcggcgacct cgacggcggc ccactcgagt tcggccacga
    12001 cgtcggggtg cacacatccc acctcgtcca tcgggaggac gtcgtggtgc atgccggaca
    12061 cgacgtcgag gcacccgttg tcgaccgtcg cgtcgtcgat ggcgaccatg cacgacacgt
    12121 gcgacgcgac gaacgggtag gccggcgcgt cctggtgcgg cgcgtacccg gcgccgcccg
    12181 gcagcttgta gttgatcttc tccttgtaga gcacggcctg ctcgccgagg agggccgacg
    12241 cggtgtcgag gaggggaccg gtcgtgagca gctcgcggaa gcccgggtgg aacgggatga
    12301 ggttctcgct ccggcacagc ttcgggccgt cgtcggtcat ctcgcggtgg tgcagccacc
    12361 cgtcgccgtc gtcgggccac gccgagatct cgtcgaccca cgaccgcagt gcggcgaggc
    12421 cgtcggcgtc gagggtctgc gtgaggagcc aaccgttggt gttgaagtgc tcgacggcct
    12481 cggcatctgc cgtgtcgaac acacgggcga cgaggacctg ggcacgaggg agtgcagtca
    12541 cgttgcacga tcgtagctgc cggtccctct ccggtcatcc ggagcactac cgtgcggaca
    12601 tgcgcgacgg gttcgacggg atcaccgacg atctgggcaa cgcggcgggc gacccgatgg
    12661 ccgattcgtg gaccgacgac gagctgtccg agctggcgct cggcgccgat ccccgtgagc
    12721 ggctgaccgc cgacgccacg ccgctcgacg gcagcgtccc ggcgagcgac ggcccgctgc
    12781 cggggtggta catgccggcc gtggcccggg tcgtgacgtc gccgacccgg cggatcgcag
    12841 cgctctcggt cgtcggcgcg ttcctggtga tcaatgcgct gggactgtgc gccacctacg
    12901 gcgtgatctc gatctgagct cgagatccgt gcggaaggtg ccggcccgtg tcacatctgg
    12961 caccgattgc agcagcggcg ccgctcagca ggatccgctg gccccgaagt cctgagtcca
    13021 gaattgggtc ccacccgacg agctcgcccg accgaacccg acgtggctgt aggccgggtc
    13081 gaggatgttc tcccggtgac ccggtgagct catccacgac cacatcgcga ggtcgacagt
    13141 ggtctggccg ctcgcgatgt tctcggcgag ggcggaccag ccgccgtacc cggagcggac
    13201 ggcccgatcg ccgatcctcg acccgtcggc gccgctgtgg ctcattgtcg agcgcccggc
    13261 ctgatcctcg gaatgctgct gcgctgcggc gccgagcgtc ccgcagaggg acaccgggcc
    13321 gacaccggct ccggcgcgga tcgtattgat gctgcggagc atctgcgcct gccagtcgcc
    13381 gccccccggt gcggggcacc ctgagagcac cgggacgaga ccgacgatca ggagcacagc
    13441 acaacgacgg agcatcccgt agtgatcggg tcatccggag gcgcgcttga cgtttgtggg
    13501 ccgttcgacc cacgcggcgg gaaggtgtgc ccagccgggc acgcgcctac ccgtgctgcc
    13561 agaaacggtt cgagaaggag acgatgccgc ccagcgcgac acggtcgagc gccgcgatgt
    13621 cgtcgggcgc aagctcccaa ccgagagcgc cggcgttctg ggtggcctgg tcgccgttct
    13681 tggcgccggg gatcgggacg g
//
LOCUS       DAOSOA010000050        13659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4523_length_13659_cov_6.200162, whole genome shotgun sequence.
ACCESSION   DAOSOA010000050 DAOSOA010000000
VERSION     DAOSOA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13659
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4523_length_13659_cov_6.200162"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKY13_05125"
     CDS             <1..695
                     /locus_tag="PKY13_05125"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT79344.1"
                     /translation="LVDRIDRIADGIEIAQRSRRIARESAGIGVALSVAAMVAAAFGF
                     LPPIWGAVTQEVIDVAVILNAVRAATDPRRREALGDDDADVMRHFGGEHDRLRPQLDL
                     LLRLADALTLPDADTESARRALRVLREELLPHEVAEAHELYPRLDAVLDGDEPTALMA
                     RTHLELRRLTDRLEMTLDDVGDRETPTSVERAELCRLLYGIHAILVLHNSQEEEGYFS
                     LADPVDEDPARR"
     gene            complement(876..1238)
                     /locus_tag="PKY13_05130"
     CDS             complement(876..1238)
                     /locus_tag="PKY13_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79345.1"
                     /translation="MGAAMWESDLSPHGVTLTFGAVSARIALEGSVDVATGVALVDAA
                     TSAVSRFRFLDLDLAAVTSIDGPGVRSLIKIKRFADAYGAIVSVVEASPIAGEALVAS
                     GLDADLGWTGSDRPVPGG"
     gene            complement(1381..2058)
                     /locus_tag="PKY13_05135"
     CDS             complement(1381..2058)
                     /locus_tag="PKY13_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79346.1"
                     /translation="MNAAAPSSAEADVAELRAAVGDLQALVATLLERPAAAAPVATPA
                     AAPVVGASRSSIEASDTAARIIVAANEAAEAAVMTARAEADQIIAAAKVKSFEIVGVA
                     RDLADSELTAERNRVATATEVWNGSRASLAAQLASLDASLSAYRTELAATAGAVADAI
                     AQLGDAVPAGDVPPGAESSNAAMAAPVPGDAPMRSSRSGSAVSAGPSAAFAVGSTHQQ
                     RAGVFGR"
     gene            complement(2391..3524)
                     /locus_tag="PKY13_05140"
     CDS             complement(2391..3524)
                     /locus_tag="PKY13_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79347.1"
                     /translation="MARSSRWRDAAAQAQSQPASPAPDAPAHPMPPVNAPVAGPPMPP
                     FAPPVPVAGPSPRINPFAPPAPPAPPAAPVAAVASASELPEPPAPSAPSATPSPPPTS
                     SAPTAGSPVEADVAELRATLQQLQTTVSALVERSHEPSVPTGTPGTDQASRILVMANR
                     AAESTLEEARAEAAEILAAAQAHSVEIISAARELADQELAAERVRVAAATSEWTERRA
                     QISAHLRDLDAALNGYRDGLGETATAIREAAVRLEAERAEPPVIDELPAPDPAAFVSA
                     HLAPNPDAVVDLTDAPEPGAVADTSGDSADSSDSADSTDELAASPRTSLFGGAVATGV
                     PGGPLAAEAPADAAVAPPEPPVADDAPVEEPPSRNLRPGLFGR"
     gene            complement(3630..6134)
                     /locus_tag="PKY13_05145"
     CDS             complement(3630..6134)
                     /locus_tag="PKY13_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT79348.1"
                     /translation="MAPQQSLTRVDLLGPGTDAGTLAAVAAGLPDGILVVDSMATALW
                     ANRAAERLFGISLADGLGLNGFDLIHPDDLQMAVLSLSSVQDKEVGTPLELRLRAHDG
                     WRLVEIVGAPLGDLLVLSIRDLTDRRRWEVAHDEDARFRSLMHNASILTMLIGGDGIV
                     EASTSALSRMLGHDQEGMEGRHLGEIVEVWDRSRLAEAMAFVLAPTSGSGVPTITVDL
                     DFHRRLGGTIPMAVTIKNLLDDPTVSALVVSSHDITDRKAAEHALLSANSVLAATLES
                     TADGILVVDTEGRITSVNRRFVELWSIPPELIASREDSRTVAFAMSQLRNPEGFVAKI
                     RDLYDDPEAESHDELELIDGRVFERDSLPQRIDGQVVGRVWSFRDVTEHRVLQEELTR
                     QAFHDALTGLANQALFRDRVDHAVARLDRTGGQLAVLFVDLDDFKHVNDSLGHTVGDE
                     LLVAISDRLARNLRSSDTAARLGGDEFAVLIEDLDDEAQAIAVAERIVASLREPVTLA
                     AKPVTISASVGIAFGRARLGLDSDALLRNADLAMYTAKARGKSCYRVFAPEMHLAAVE
                     RLEVETCLRGAARRGELVVHYQPIYDLATGRMRAAEALVRWQHPERGLLAPGAFIALA
                     EESDLIVEIGDHVLGAALAEARAWQLAVGDDLAPAVNVNLSPRQLLDPTLPERIGDQV
                     RAAGLRPELLILEITEGALMADPAMAAQNLRRLSESGVQLAVDDFGTGYSSLAYLQQF
                     PIDFLKVDGCFVNQMLDRSDAGLVDAIVQLTHTLGLTPIAEGIEFASQVERLQAMGCD
                     LGQGYHLDRPLDATTVRERIIANAGGPTSLRLDPIC"
     gene            6250..6441
                     /locus_tag="PKY13_05150"
     CDS             6250..6441
                     /locus_tag="PKY13_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79349.1"
                     /translation="MPEPSDPSPETHRRRWGRPAPHKAVEPELRNAAVFTDSRVEIEI
                     DDETGKPQLRIVRGARRKE"
     gene            complement(6455..7054)
                     /locus_tag="PKY13_05155"
     CDS             complement(6455..7054)
                     /locus_tag="PKY13_05155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT79350.1"
                     /translation="MIVVVRHGRTVSNAAGLLLGRADPDLDELGIDQARRLGAALGHE
                     TAPAVDRIVTSPLARTLQTAAAVAAATGAAVTVDERFVELDYGEWDQRPLADVAADEW
                     AAWRADVAFAPPGGESLAELGRRVRSGLDDLAEEARERTIVVVTHVSPIKAAVAWAVG
                     GTDEMAWRMFVAPASITRIATSVGAPSLHTFNDCSHLAG"
     gene            complement(7086..7592)
                     /locus_tag="PKY13_05160"
     CDS             complement(7086..7592)
                     /locus_tag="PKY13_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79351.1"
                     /translation="MLIWFAAGSVAIVWVIFRSPAVDYRVVALGSVLALVEVPIGVGP
                     FQTLMLSVVALALTMGLTVGQRLRRRRWLGIPIGMFLHLVLDASWSDQSVFWWPFGGW
                     TFPDVSAPVIQRGIWSLLLELVGCMVALWLWGEFGLDDPERRRRLWRTGQLDRSLMRR
                     RGVPEDDE"
     gene            complement(7657..8370)
                     /locus_tag="PKY13_05165"
     CDS             complement(7657..8370)
                     /locus_tag="PKY13_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOT79352.1"
                     /translation="MDLEQLLALQELDTRLAQLGHRLTHDPALANLAAAQAAVADVER
                     RELELFGERDVVRQELKRLEDEASLTEEKIAKVNDTLYGGVVTAHKELESLQHELAML
                     KERLGGFEDLELEQMELAEPLEARATALAAEKTAAIANVGDAEMGVTAMQAEVGAELD
                     TVTSAREAQAATLPESLLAQYDKLRTALGGQGAARLAPGGRCEGCHLTLPRAEYDAIK
                     HEPPDAVHLCPECGRILVR"
     gene            complement(8420..10597)
                     /locus_tag="PKY13_05170"
     CDS             complement(8420..10597)
                     /locus_tag="PKY13_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOT79353.1"
                     /translation="MANLRRGTSPHPRSPIRLLARAVFAFALGGASIAATMGLTWNVA
                     SQSGRAISTSPSEPILLKPLATRSIVYDAGGNEMALLYAEQDRQEVPLESVPELVQTT
                     ILTSEDADFYSHKGVNVRSLVRALHSDIDSGQVAQGGSTITQQLIKQSITGDRQTLER
                     KVREAILATKLEKQMSKDEILERYLNTVYLGHGAYGVEAASETYFNKSVTDIGWAEAA
                     MLASLIPNPTRYDPISTETRDRAKTQRGIVVRRLVQEQVIDQAAADKINDTPLPTQVF
                     EREKATAQAQLVGGSYFSETVKQVLLDLPELGATAQDRYDAVFTGGLRVYTTYEPAAQ
                     QAAEAATLKVPDSGGKYFVGVAAVDPKNGAVRAMVGGPDFETSKVNYVTQGWRQPGSS
                     FKYFVLMAAIEAGYVPNDTISGASPCKFPDPSAKDGVYVATGGGKTTTITGQTQVSSN
                     CGFLRLGQTVGLDRVADMANRMGMSTIESYTGDDGSTGLRETPIPSDILSLPLGSKEV
                     HPVNMAAAYATAANDGIYNPPYYVDKVTDAKGKVLYQHQSEGTRVVSVETARLVTQVL
                     AKNVTGGTGRKSALKEQESAGKTGTTQENADTWFVGYTPYLSTAVWLGSPTGRDKIVI
                     KGRAVMGGDYTAQVWQAFNEPYHALLPPIDFEPPEPTRKGKAIRYTNKVDKGGTTKKP
                     TTTTTVPQGFPPGFPPAAPQTSPPATTPPTTPAGDGGTATGGN"
     gene            10705..11643
                     /locus_tag="PKY13_05175"
     CDS             10705..11643
                     /locus_tag="PKY13_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOT79354.1"
                     /translation="MAHDRRFRFGIQLSQPLAGLSWAESARKAEDLGFSTLFLPDHFG
                     DQLAPIPAMMAAADATTTLNVGALVFDNDYKHPVVMAKEIATIDQLSGGRVEFGLGSG
                     WMKSDYDESGIPYDAPKVRVERFFEGVEIIKGLWSEEPVNFSGEHYTITGLNGLPKPA
                     RAGGPPLLIGGGAPRMLRFAGAHADIVGVNPSIHSGAIDGEAARDGAADRFDQKLEWV
                     REGAGDRFDDIELNVLVFLAQITDDATGVAEMMAPLFGTTPDLMAEAPLALFGTEDGI
                     CETIEARRERWGLSYYVFQGDALDTMAPIVARLAGN"
     gene            11646..12662
                     /locus_tag="PKY13_05180"
     CDS             11646..12662
                     /locus_tag="PKY13_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT79355.1"
                     /translation="MTRLAIAGAGWAGRVHTLAASAVSGATVPLIATRSVGSAEVLAS
                     EIGARPVTADQLPGSAEAVVVATPPPDHAALAVRLLSAGTAVLIEKPLAATLTEADAI
                     VAAAEATGAAACYAENLLFAPALDVAVNRRVALGAIDHLEVRMAQPMPTWGHFAEPLT
                     AGGVLFDLGVHALAVLLVLARDDEPVALRARLGSSRDDGADDVGRVEVRFASDLIASI
                     DVSWNSDVIEWSAEASSPTGVVRVDFQPDVTVEVNGTSVPVPHVDSEIDARIFDLGYH
                     AQLEGFCSVVARRGGRVCPAGFGRSMLEMVCAAYVSAGRDGDEVLLPYDGPRDVTPLQ
                     LWRG"
     gene            12775..13545
                     /locus_tag="PKY13_05185"
     CDS             12775..13545
                     /locus_tag="PKY13_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT79356.1"
                     /translation="MDEILHLLADQQQELTDLLGGRTDDEWHQPSACAGWSVGDIVLH
                     LIQTNDMAIASCEGRFDELIRSPLYAPDESVTSLDDVVDLAVRRARGATPAELYEQWQ
                     DGAQRLIGALDAAGAGTRVPWVVGTLSAKTLATTRIAETWIHTGDVAVGLGVELAPTD
                     RLFQIARLSWRTLPHAFGRVGLGPPGPVGFDLVGPHGDAWQFGMALDDGTAPPTVISG
                     TGLDLVRVASQRYTADQTGLVGTGPDAEQVLRLVRSWA"
BASE COUNT         1976 a         4816 c         4821 g         2046 t
ORIGIN      
        1 tcctggttga tcgcatcgat cggatcgccg acggcatcga gatcgcgcag cggtcgcgac
       61 gcatcgcccg cgagagcgcc ggcatcggag tcgcgctgtc ggtcgcggcg atggtcgctg
      121 cggcgttcgg gttcctccca cccatctggg gagcagtcac ccaggaggtc atcgacgtgg
      181 cggtcatcct caacgccgtc cgcgctgcga ccgacccccg gcgccgggag gcgctcggcg
      241 atgacgacgc cgacgtgatg cgccacttcg gaggcgagca cgaccggctc cggccccagc
      301 tcgacctcct cctccggctc gccgacgctc tgaccctgcc tgacgccgac accgagtcgg
      361 cgcgacgggc gctccgagtc ctccgggagg agctcctccc ccacgaggtc gctgaagcgc
      421 acgagctgta cccccgactc gacgcggtcc tcgacggcga cgagcccaca gccttgatgg
      481 cgcgcaccca cctcgaactc cgcaggctga ccgatcggct cgagatgacg ctcgacgacg
      541 tcggggaccg ggagacaccg acctcggtcg aacgagccga gctgtgccga ctgctgtacg
      601 gcatccacgc catcctcgtg ctgcacaaca gccaggagga ggagggctac ttctcgctcg
      661 ccgatcccgt cgacgaggat ccggctcgac gctgagtcac cccgaggggc cgggagaacg
      721 agagagcccc ccgctgctca caacgggggg cttctcgggc tgtgcgggaa gggctgggag
      781 aaacccttgc tgtggacctg atgcccacac gtcggcgacc ccaaacatcg ggagtcacga
      841 aatcggatga ttccccgacc ctcgcccgac ggctcctacc cgccgggcac gggacggtcc
      901 gagccggtcc acccgaggtc ggcgtcgagt cccgacgcca cgagcgcttc ccccgcgatc
      961 ggcgacgcct cgaccaccga gacgatggcg ccgtaggcgt cggcaaaccg cttgatcttg
     1021 atcagtgagc ggacacccgg cccgtcgatg gaggtgacgg cggcgaggtc gagatcgagg
     1081 aatcggaagc gggagacggc gctggtggcg gcgtcgacga gcgctacccc ggtggcgacg
     1141 tcgacgctcc cctcgagcgc gatccgggcg ctcaccgctc cgaacgtcag ggtcacccca
     1201 tggggcgaca gatcgctctc ccacatcgct gcgcccatgc gccccctatc gagggcaagg
     1261 cccgccggcc ccgccgttgt ccgccgtctc gatccatccc gacagcgcac ccgccgcgcc
     1321 accggaactc acgtcaagct aacggtgaca ggccccggtc gccagtgtgg atgtcggaga
     1381 tcagcggccg aagaccccgg cgcgctgctg gtgggtcgag ccgacggcga acgctgcgct
     1441 cgggccagcc gagaccgcgc tgccggagcg actgctgcgc atcggcgcgt cgccgggaac
     1501 cggagcggcc atggccgcat tcgaactctc cgcgccaggc gggacgtcac cggccgggac
     1561 cgcatcgccg agctgggcga tcgcgtcggc gacagcaccg gcagtcgccg cgagttcggt
     1621 tcggtacgcc gacagtgagg catcgagcga cgcgagctgc gccgcgaggc tcgcacggct
     1681 gccgttccac acctcggtgg cggtggcgac ccgattgcgc tccgcggtca gttcgctgtc
     1741 cgcgaggtcg cgggcgacgc cgacgatctc gaagctcttg accttcgctg cggcgatgat
     1801 ctggtcggct tcggcacggg ccgtcatcac tgccgcctcg gccgcctcgt tcgcggccac
     1861 gatgatgcgt gcagcggtgt cggacgcctc gatcgacgat cgggaggcgc cgacgacggg
     1921 cgcggcggcc ggagtagcca ccggagcggc tgcggcggga cgctccagga gcgtcgcaac
     1981 cagcgcctgc aggtcgccga cggcggcccg cagctcggcg acgtcggcct cggcgctgct
     2041 cggtgctgcg gcgttcatcg gggtggcccc agacgcggca gccggcgtgg cgacggccgg
     2101 agacgcaacc gccgccggga cgaagggcgg gggcgcgggg gccacagcgg gggtcacagc
     2161 gggggtcggc tgcgcaacgg cgggcggagc gggcaacgcg gggcccgtcg accggtccca
     2221 agccgccacc tgcttgcgtc gagatccgat taccataggg tcctccgaga cgagtgagca
     2281 ctttcagtgc ttttcacaac gtttcaaacc ttaacggccg aatctgccga tgcaaccggt
     2341 tctccgaggt gccgatgacc tggcacgggc ccggccggcg tcgcgtgcgc tcagcggccg
     2401 aacagaccgg gccggaggtt gcgcgacggc ggctcctcca ccggcgcatc gtccgccacc
     2461 ggcggctccg gtggcgccac cgcagcgtcg gcaggggcct cggcggcgag cgggccgcca
     2521 ggcacccccg tggcgaccgc accaccgaac aggctcgtcc gcggtgacgc cgcgagctcg
     2581 tcggtggaat cggccgaatc gctcgaatcg gccgagtcac ccgaggtgtc ggcaacggcg
     2641 ccgggttcgg gggcgtcggt caggtcgacc accgcatcgg ggttcggcgc gaggtgggcg
     2701 ctgacgaagg cggccggatc cggggccggc aactcgtcga tcaccggcgg ctcggcccgc
     2761 tcggcctcga ggcgaacggc cgcctcgcgg atggctgtgg cggtctcgcc gagcccgtcg
     2821 cggtacccgt tcaacgccgc gtcgaggtcg cgcaggtgtg cggagatctg cgcccgacgc
     2881 tcggtccact ccgaggtggc cgcagccaca cggacccgtt cggccgccaa ctcctggtcg
     2941 gcgagctcac gcgccgccga gatgatctcg acgctgtggg cctgggcggc ggccaggatc
     3001 tccgcggcct cggcacgagc ctcctcgagg gtcgactctg cggctcggtt cgccatgacg
     3061 aggatgcgcg acgcctgatc ggtgcccgga gtgcccgtcg gcaccgacgg ttcgtgggac
     3121 cgctcgacga gcgccgacac ggtggtctgc aattgctgga gcgtcgcccg caactcggcg
     3181 acatcggcct cgaccggcga gccggccgtc ggcgccgagg acgtcggagg cggcgatggg
     3241 gtcgctgacg gtgctgacgg tgctggcggt tcgggcagct cggacgccga cgccaccgcc
     3301 gctacggggg cggcgggcgg ggccggaggg gccggagggg cgaacgggtt gatccgcggg
     3361 ctcggcccgg cgacgggaac cggcggggcg aacggtggca tcggcggacc ggcgaccggc
     3421 gcgttcaccg gcggcatggg gtgggccggg gcgtcggggg ccggcgacgc gggctgcgac
     3481 tgcgcctgcg ccgcggcgtc cctccaccgg ctacttcttg ccattccacc ctccagaaca
     3541 cgatgcgttt cggtgcgatt catgcaaccc tgggcaaggt aaccacggtt cgaccgctcg
     3601 tcaccacttc cgtgcagaga tagcgatctt cagcagatcg gatcgaggcg gagcgacgtg
     3661 ggtcccccgg cgttcgcgat gatccgctcg cgcaccgtcg tggcatcgag gggccggtcg
     3721 aggtggtacc cctgcccgag gtcgcagccc atggcctgca gccgctccac ctgcgacgcg
     3781 aactcgatcc cctccgcgat cggcgtgagg ccgagcgtgt gggtgagctg cacgatcgcg
     3841 tcgacgaggc ccgcatcgga ccggtcgagc atctggttca cgaagcagcc gtccaccttc
     3901 aggaagtcga tcgggaactg ctggaggtag gccaacgagg agtacccggt gccgaagtcg
     3961 tcgaccgcga gctggacccc cgactcgctc agccgacgga ggttctgcgc cgccatcgcc
     4021 gggtcggcca tgagggcgcc ctcggtgatc tccaggatca ggagctcggg acgcaggccg
     4081 gccgcccgga cctggtcacc gatccgttcc ggcagcgtcg ggtcgagcag ctgccggggc
     4141 gacaggttga cgttgacggc cggggcgagg tcgtcgccga ccgccagctg ccacgcccgg
     4201 gcctcggcga gtgcggcgcc cagcacgtgg tcgccgatct cgacgatcag atcgctctcc
     4261 tcggcgaggg cgatgaatgc ccccggggcg aggagcccac gttcgggatg ctgccaccgg
     4321 accagcgcct ccgccgcccg catccggccg gtggccaggt cgtagatcgg ctggtagtgc
     4381 acgaccagct cgccgcgccg ggcggcacca cgcaggcacg tctccacctc cagacgctcg
     4441 acggcggcaa gatgcatctc gggggcgaac acccggtagc agctcttccc ccgagccttg
     4501 gcggtgtaca tcgcgagatc ggcgttgcga aggagggcgt cactgtcgag gccgagccgg
     4561 gctcgcccga aggcgatccc cacgctggcg ctgatggtga cgggcttcgc tgcgagcgtc
     4621 accggctcac ggagcgaggc gacgatccgt tcggcgaccg cgatggcctg cgcctcgtcg
     4681 tcgaggtcct cgatgagcac cgcgaactcg tcgccgccga gtcgggcggc ggtgtccgac
     4741 gaacggaggt tgcgggcgag ccgatcgctg atcgccacca gcagctcgtc gcccaccgtg
     4801 tggccgaggc tgtcgttgac gtgcttgaag tcgtcgaggt cgacgaagag caccgcgagc
     4861 tgcccgccgg tgcggtcgag acgcgccacg gcatgatcca cccggtcacg gaacaacgcc
     4921 tggttcgcga gccccgtcaa ggcatcgtgg aacgcctggc gcgtcagctc ctcctggagg
     4981 acgcggtgct cggtgacgtc gcggaagctc cagacgcgcc cgacgacctg tccgtcgatg
     5041 cgctgtggga gcgagtcgcg ttcgaagact cggccgtcga tcaactcgag ctcgtcgtgg
     5101 ctctccgcct cgggatcgtc gtagaggtct cggatcttcg cgacgaaccc ctcgggattg
     5161 cggagctggg acatggcgaa cgccacggtc cgggagtcct cgcggctcgc gatcagctcc
     5221 ggcgggatcg accacagctc gacgaaacgg cggttcacac tcgtgatgcg cccctcggtg
     5281 tcgacgacga ggatgccgtc ggccgtcgac tccagggtgg ccgccagcac cgagttcgcg
     5341 gacagcagcg cgtgttccgc cgccttgcga tcggtgatgt cgtggctcga gaccaccagc
     5401 gcgctcacgg tcgggtcgtc gagcaggttc ttgatcgtca cggccatggg gatcgtgccc
     5461 ccgagccgac ggtggaagtc gagatcgacg gtgatggtcg gcacacccga gcccgacgtc
     5521 ggggccagga caaacgccat cgcctcggcc agccgcgagc ggtcccagac ctcgacgatc
     5581 tcaccgagat ggcgcccctc catcccctcc tggtcatggc cgagcattcg gctgagcgcc
     5641 gacgtcgacg cctcgacgat gccgtcgccg ccgatcaaca tcgtgaggat cgacgcgttg
     5701 tgcatgagcg accggaaccg agcgtcttcg tcgtgggcga cctcccaccg tcgtcggtcc
     5761 gtcaggtcgc ggatgctcaa cacgagcagg tcgccgagcg gcgctcccac gatctcgacg
     5821 aggcgccagc cgtcgtgggc gcgcagccgc agttcgaggg gtgtgcccac ctccttgtcc
     5881 tgcacactcg acagcgagag cacggccatc tgcagatcgt cggggtggat gaggtcgaat
     5941 ccgttcagcc cgagaccgtc ggccaacgag atgccgaaca gccgctccgc agcccggttc
     6001 gcccacaggg cggtggccat cgagtcgacg accaggatcc cgtcggggag accggcggcg
     6061 accgcggcga gggtgccggc gtccgtcccc ggtcccagga ggtcgacgcg tgtgagcgac
     6121 tgctgcggag ccatcgtctg aatctcggca gcttcgccgt gcggattgat ctttgtcctg
     6181 caccttcggc ccaccggggc gaggtgaagg cgctcaaggc aaacgggccg gaataccgat
     6241 gcagagccca tgcctgagcc ttccgaccca tcaccggaga cgcatcgacg ccgttggggg
     6301 cgccccgcac cccacaaggc ggtggaaccc gagctgcgaa atgccgccgt cttcaccgac
     6361 agtcgcgtgg agatcgagat cgacgacgag acggggaaac cccagctccg gatcgtgcgc
     6421 ggcgcccgtc gcaaggagta gcgacgcgcc gggttcagcc ggccaggtgg gagcagtcgt
     6481 tgaacgtgtg gagcgacggc gcaccgaccg acgtcgcgat cctggtgatg gaggcgggcg
     6541 ccacgaacat ccgccacgcc atctcgtcgg tgccgcccac ggcccaggcc accgcggcct
     6601 tgatcgggga cacgtgtgtg accaccacga tcgtgcgctc gcgggcctcc tcggcgaggt
     6661 cgtccaaccc cgagcgcacc cggcggccca gttcggcaag ggattcgccg cccggcgggg
     6721 cgaaggcgac gtcggcgcgc cacgccgccc actcgtcggc ggcgacgtcg gccaacggcc
     6781 gctggtccca ctcgccgtag tcgagctcga cgaatcgctc gtcgacggtc acggcggcac
     6841 ccgtcgccgc agcgaccgcc gccgcggtct gcagcgtgcg ggcgaggggc gacgtgacga
     6901 tccggtcgac ggccggggcg gtctcgtggc cgagcgccgc tccgagccgc cgcgcctggt
     6961 cgatgccgag ctcgtcgagg tcggggtcgg cgcggcccaa caacaggccg gcggcgttcg
     7021 acaccgttcg tccgtgccgt acgacgacga tcattccccg gtcctcctcg gcgctcggcg
     7081 ccgcatcact cgtcgtcctc ggggacgccg cgacggcgca tcagggatcg gtcgagctgg
     7141 cccgtccgcc acaaccgccg gcgtcgctcg gggtcgtcga gcccgaactc gccccacaac
     7201 cagagggcga ccatgcaccc caccaactcg aggagcaggg accagatacc ccgttggatg
     7261 accggtgccg acacgtcggg gaacgtccat ccgccgaacg gccaccagaa caccgactgg
     7321 tcggaccacg acgcgtcgag cacgagatgg aggaacatcc cgatggggat gcccaaccac
     7381 cgtcgccgcc ggagtcgctg cccgaccgtg agccccatgg tgagcgccag ggcgaccacc
     7441 gacagcatca gggtctggaa cgggccgacg ccgatcggca cctcgaccaa cgccagcacc
     7501 gacccgagcg cgacgacgcg gtagtcgacc gccgggctcc ggaagatcac ccacacgatc
     7561 gccacactgc cggcggcgaa ccagatcagc attcccggtg cgtcgaaccc agggagcgag
     7621 ccagcgcggc ggcgcccgag gtcatcgaac ccgccgtcat cgaacgagga tgcggccgca
     7681 ctcggggcag aggtggaccg cgtcgggcgg ctcgtgcttg atggcgtcgt attcggcgcg
     7741 cggcagggtc agatggcatc cctcgcaacg gccaccggga gcgaggcggg cggcgccctg
     7801 cccgccgagc gcggtgcgga gcttgtcgta ctgggcgagc agcgactcgg gaagcgtggc
     7861 ggcctgcgcc tcgcgcgccg acgtcacggt gtcgagttcg gcgccgacct ccgcctgcat
     7921 ggcggtgaca cccatctcgg cgtcgccgac gttggcgatc gcagcggtct tctccgctgc
     7981 caaggcggtg gcccgggcct cgaggggctc ggcgagctcc atctgttcga gctcgagatc
     8041 ctcgaacccg cccaggcgtt ccttgagcat cgcgagttcg tgctggagcg actcgagctc
     8101 cttgtgcgcg gtgacgaccc cgccgtagag cgtgtcgttg accttggcga tcttctcctc
     8161 ggtgagcgac gcctcgtcct ccaaccgctt cagctcttga cgcacgacgt cgcgttcgcc
     8221 gaacagttcg agctcacggc gctcgacatc ggcgaccgct gcttgcgccg cggcaaggtt
     8281 ggcgagggcc ggatcgtggg tgagccggtg accgagctgc gccagacgcg tgtcgagctc
     8341 ctgcagggcc aacaactgtt cgagatccaa cgggtgtcct ttccgagcct gggcgggaac
     8401 ctatcgcacc ggcgtcggat cagttgccgc cggtggccgt cccgccgtca ccggcggggg
     8461 tggtcggcgg cgtggtcgct ggtggactgg tctggggcgc ggccggcggg aaccccggtg
     8521 ggaacccctg cgggaccgtc gtggtggtcg tgggcttctt cgtcgtgccg cccttgtcga
     8581 ccttgttggt gtagcggatg gccttgccct tgcgtgtggg ctcgggcggt tcgaagtcga
     8641 tcggcgggag gagcgcgtga tacggctcgt tgaacgcctg ccacacctgc gccgtgtagt
     8701 cgccgcccat cacggcgcgg cccttgatga cgatcttgtc gcggccggtg ggcgagccca
     8761 gccacactgc tgtcgacagg tacggcgtgt acccgacgaa ccacgtgtcg gcgttctcct
     8821 gggtggtgcc ggtcttgccg gccgactcct gctccttcaa cgccgacttg cgaccggtgc
     8881 caccggtgac gttcttcgcg agcacctggg tgaccagtcg ggcggtttcg accgacacga
     8941 cccgggtgcc ctcggactga tgctggtaca gcaccttgcc cttggcgtcg gtgaccttgt
     9001 cgacgtagta gggcgggttg tagatgccgt cgttggccgc cgtcgcgtag gcggccgcca
     9061 tgttcaccgg gtgcacctcc ttgctgccga ggggcagcga caagatgtcg ctcgggatcg
     9121 gggtctcgcg gagaccggtc gagccgtcgt cgccggtgta ggactcgatc gtcgacatgc
     9181 ccatgcggtt ggccatgtcg gcgacgcggt cgagcccgac ggtctggccc aggcgcaaga
     9241 acccgcagtt ggacgacacc tgcgtctggc cggtgatggt cgtcgtcttg ccgccgcccg
     9301 tggcgacgta cacgccgtcc tttgcgctcg ggtcggggaa cttgcacggc gacgcgcccg
     9361 agatcgtgtc gttgggcacg tagcccgctt cgatcgccgc catcagcacg aagtacttga
     9421 acgacgaacc gggctggcgc cacccctggg tgacgtagtt gaccttcgag gtctcgaaat
     9481 cgggaccgcc gaccattgcc cggaccgcac cgttcttcgg atcgacagcc gccacgccga
     9541 cgaagtactt gccaccgctg tcgggcacct tgagcgtcgc cgcctcggcc gcctgctgcg
     9601 ctgccggctc gtaggtggtg tacacccgca ggccgccggt gaacacggcg tcgtaacggt
     9661 cctgcgccgt cgcgccgagc tccggaaggt cgagcaggac ctgcttcacc gtctcggaga
     9721 agtagctgcc acccaccagc tgggcctggg ccgtggcctt ctcgcgttcg aagacctggg
     9781 tcgggagcgg cgtgtcgttg atcttgtcgg cggccgcctg gtcgatcacc tgctcctgga
     9841 cgaggcggcg cacgacgatg ccgcgctggg tcttcgctcg gtcacgggtc tcggtggaga
     9901 tcgggtcgta gcgcgtcggg ttgggaatca gcgacgcgag catcgccgcc tcggcccagc
     9961 cgatgtcggt cacgctcttg ttgaagtacg tctccgacgc cgcctcgacg ccataggcac
    10021 cgtggccgag gtacacggtg ttgaggtacc gctcgaggat ctcgtccttg gacatctgct
    10081 tctcgagctt cgtggcgagg atcgcctcgc gcaccttgcg ttcgagcgtc tgacggtcgc
    10141 ccgtgatcga ctgcttgatg agctgctggg tgatcgtcga cccgccctgc gcgacctggc
    10201 ccgaatcgat gtcgctgtgc agcgcccgga ccagcgagcg cacgttgaca cccttgtggg
    10261 agtagaagtc ggcgtcctcg ctcgtcagga tggtcgtctg cacgagttcg gggacgctct
    10321 cgagcggcac ctcctggcgg tcctgctcgg cgtacagcaa cgccatctcg ttgccgccgg
    10381 cgtcgtagac gatggagcgg gtcgccaagg gcttgagcag gatcggctcg gacggcgagg
    10441 tcgagatcgc acgcccggac tgcgacgcca cgttccaggt gagccccatc gtcgcggcga
    10501 tcgacgcgcc gccgagggcg aacgcgaaca cggcgcgcgc caacagcctg atcggggaac
    10561 ggggatgcgg ggaggtgccc cgtcggaggt tggccacagc gaccccagga tagttgtcgg
    10621 gtgagtccgg acttctgcac ccgctggtat cgtcgcccgc gatcgcctcg atcgcctcga
    10681 ccgacctcct gggggacgac ttccatggcc cacgaccgcc gcttccgctt cggtatccaa
    10741 ctgtcccagc cgctcgccgg cctgagctgg gccgagagcg ctcgcaaggc cgaggacctg
    10801 gggttctcca cgctgttcct gcccgaccac ttcggcgacc aactggcccc gatccccgcg
    10861 atgatggccg ccgccgacgc cacgaccacc ctcaacgtgg gtgcgctcgt gttcgacaac
    10921 gactacaagc accccgtggt gatggccaag gagatcgcca cgatcgacca gctgtcgggc
    10981 ggccgggtcg agttcgggct cggctcgggg tggatgaagt ccgactacga cgagtcgggc
    11041 atcccctatg acgcccccaa ggtccgcgtc gaacgcttct tcgagggcgt cgagatcatc
    11101 aagggcctgt ggtcggagga gcccgtgaac ttctcgggcg agcactacac gatcaccggc
    11161 ctcaacggcc tccccaagcc ggcccgcgcc ggtggtccgc cgctgctcat cggtggcggc
    11221 gcacctcgca tgctccgctt cgcgggcgcc cacgcggaca tcgtcggggt gaacccgtcg
    11281 atccattcgg gcgcgatcga cggcgaggcg gcacgcgacg gcgccgccga ccgtttcgac
    11341 cagaagctcg agtgggtccg ggagggcgcc ggggaccgtt tcgacgacat cgaactcaac
    11401 gtgctcgtgt tcctcgcgca gatcaccgac gacgccaccg gcgtcgccga gatgatggcg
    11461 ccgctcttcg gcaccacccc cgacctcatg gcggaggccc cgttggcgct gttcggcacc
    11521 gaggacggga tctgcgagac gatcgaggcc cgtcgggagc ggtggggtct ctcgtactac
    11581 gtgttccagg gcgacgccct cgacaccatg gcaccgatcg tcgcccgcct cgccggcaac
    11641 tgagcgtgac gcgcctcgcg atcgccgggg ccggttgggc cggccgcgtc cacacgttgg
    11701 cggcgtccgc cgtcagtggc gccaccgtgc cgctgatcgc gacccggtcg gtcgggtcgg
    11761 ccgaggtcct cgcgtcggag atcggcgccc gccccgtgac cgccgaccag ctccccggta
    11821 gcgccgaggc cgtggtcgtc gcgacgccac cgcccgacca tgcggcgctg gccgtccgcc
    11881 tgttgtcggc cggcacggcg gtcctcatcg agaagccgct cgccgcgacg ctcaccgagg
    11941 ccgacgccat cgtggcggcc gccgaggcca ccggtgcggc ggcctgctac gccgagaacc
    12001 tcctcttcgc cccggcgctc gacgtggccg tgaaccgccg ggtcgccctc ggagcgatcg
    12061 accacctcga ggtgcgcatg gcgcagccga tgccgacgtg gggtcacttc gccgaaccgt
    12121 tgaccgccgg cggtgtgctg ttcgacctcg gggtgcacgc gctggcggtg ttgttggtgc
    12181 tggcccgcga cgacgagccc gtggcgctcc gggcccgact ggggtcgagc cgtgacgacg
    12241 gcgccgacga cgtcgggcgg gtcgaagtcc gcttcgcctc cgacctgatc gcgtcgatcg
    12301 acgtgagttg gaacagcgac gtcatcgagt ggtcggcgga ggcctcctcg cccaccggcg
    12361 tcgtccgcgt cgacttccag cccgacgtca ccgtcgaggt caacggcacg tcggtgccgg
    12421 tcccccacgt cgactccgag atcgacgccc gcatcttcga cctcggctac cacgctcagc
    12481 tcgaggggtt ctgctccgtc gtcgctcgac gcggcggccg cgtgtgtcct gccggcttcg
    12541 gccggtcgat gctcgagatg gtgtgcgccg catatgtctc ggcgggccgc gacggcgacg
    12601 aggtgctgct gccctacgac ggccctcgcg acgtcactcc cctgcagctc tggcgcgggt
    12661 agtccgtcac cgccccgtcg tcaccccccg ccattctgcc cccgtcgttt tgccctgcag
    12721 aacgaccggt ttgccgattg aatcgcggga cagaacgcga cgcgaggggg tgacatggac
    12781 gagatactgc acttgctcgc cgaccagcag caggagctga ccgatctgct cggtggtcgg
    12841 accgacgacg agtggcatca accgtcggcg tgtgctggct ggagcgtcgg cgatattgtg
    12901 ctccacctca tccagacgaa cgacatggcg atcgccagct gcgaggggcg cttcgacgag
    12961 ctgatccggt cgccgctcta cgcgcccgac gagagcgtaa cgtcgctcga tgacgtggtg
    13021 gacctggccg ttcggcgtgc acgtggcgcc acgccggcgg agctgtacga gcagtggcag
    13081 gacggagcgc agcgactcat cggcgcgctc gacgcagccg gcgccggcac ccgggtgccg
    13141 tgggtcgtcg gcacgctgtc ggcgaagacg ctcgccacga cccgaatcgc ggagacgtgg
    13201 atccacaccg gtgacgtcgc cgtcggcctc ggggtcgagc tggcgccgac cgatcggctc
    13261 ttccagatcg cccggctctc gtggcggacc ctcccccacg cattcggccg tgtcggcctc
    13321 gggccgcccg gccccgtggg cttcgacctc gtcggcccgc acggcgatgc ctggcagttc
    13381 ggcatggcgc tcgacgacgg gaccgcgcca ccgaccgtca tctccgggac cggcctcgat
    13441 ctcgtccggg tcgcgtcgca gcggtacacc gccgatcaga ccggcctggt cggcaccggt
    13501 cccgacgccg agcaggtcct ccggctcgtg cgctcctggg cctgagaacg ccgaaggccc
    13561 cctcgcgggg gccgtcgggg tctcgcatgt cttggcaggc agcgatctgg atcgtgactc
    13621 ggatcagccg aggttcggag ccgtaagaag ctcccgacg
//
LOCUS       DAOSOA010000051        13478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4595_length_13478_cov_5.975192, whole genome shotgun sequence.
ACCESSION   DAOSOA010000051 DAOSOA010000000
VERSION     DAOSOA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13478
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_13478_cov_5.975192"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1332)
                     /locus_tag="PKY13_05190"
     CDS             complement(244..1332)
                     /locus_tag="PKY13_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HOT79357.1"
                     /translation="MTTPTTPATASTSDAPRITGSTRLALLMHGHLRQYTGKMGFGLL
                     RYSPGTVTSVIDADEAGGSLREVAGIDSDAPIVADAEAAAAIGADVLVIAVATSGGVL
                     PAGYRSEIVTALRSGMSLINGLHGRFAEDPEFAAALQPGAFIWDVRREPEGLVSGTGR
                     ARALSCRRILTVGTDMAIGKMTASLELDAESRRRGLRSRFVASGQIGMCISGDGVPLD
                     AVRVDFASGAIESLCLRHGPDHDVLWVEGQGSALHPGSTAWIALLRGSMPTDLVLVHR
                     AGQRHLDGLPDVAIPPLPEVIDLYERVAAATTAYPAAKVRAIALNCGHLTSEEAADAV
                     RAVQAETGIATADVLRDGPGPLLDALLA"
     gene            complement(1329..2390)
                     /locus_tag="PKY13_05195"
     CDS             complement(1329..2390)
                     /locus_tag="PKY13_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOT79358.1"
                     /translation="MRVTSSRIVLTKRHALTISRGTMAGSTNVVVEVSHDGIVGIGEM
                     APSDVTGDSAEDTEVTLERWGPLLAPVAPVERQRIATLLEHDDAGSAVRAALDMALYD
                     WLGKRAGLPVWQLLGGDAGRIVPTSLTVGINPPDVVREVVPEILHRTGAMVLKVKLGQ
                     PAGLDADREMFAAAQESARATADRPVVWRVDANGGWDVEGALAMDRWLADRGVELVEQ
                     PLPQGHEHELPALHRGSSLPIVADESVRTSADVAALADRVDGVNLKLMKCGGLDEALR
                     IIHTARAHGLSVMIGCMGESSLAIGAGAQLAPFVDHLDLDSHLNLLDDPFDGPTFTDG
                     VVRPSDRPGLGVRWTGADA"
     gene            complement(2409..2843)
                     /locus_tag="PKY13_05200"
     CDS             complement(2409..2843)
                     /locus_tag="PKY13_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT79359.1"
                     /translation="MATPPDDGLLGGIVRLNLAVTRVLEGIAGAHGLTMADYLVLGVV
                     RGAPDGRSAPTAIADTLGRTTGGMTLTLDRLEQAGWLRRSPDPEDGRRVVIELTDGGR
                     DLAVAINTELHRWEAGLDLPHGRGEVVELVDGLTEAIGLHRT"
     gene            complement(2855..4099)
                     /locus_tag="PKY13_05205"
     CDS             complement(2855..4099)
                     /locus_tag="PKY13_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT79360.1"
                     /translation="MTATDIPTSVTPAAEDLPKIISVDDHILEPRTLWQEQLPPSLRD
                     RGPRVVREKVSLEFKGGHYGFTRGAEDGKLCDLWLFDDLVVPTGLLHAAVGYPDEEQI
                     NIPAIYEDFRPGTYDKTARLEDMTANHVEAAINYPNVFPRFAGQGFAERDDKDFALLC
                     LQIYNDWMIDDWCGADANGRLIPLTLVPLWDPVLAAAEVRRCAAKGSYAIAFSENPSK
                     LGFASMHSGEWDVLWDACQESDTTVSMHIGSSSTMPTTSPDAPLGVSMALSSQNAQGS
                     LTDWIFSGTLSRFPTLKIAFAESQIGWMPYLIERMDIVATEGAAAGVQLDRMPSEIVK
                     GRVWGCVFDDQHGLKSRDAVGLEHILFETDYPHTDGTWPHSRSVAHRLCAGAGMNEQE
                     CYEFLRGNAIRCYGLERFGISV"
     gene            complement(4096..4668)
                     /locus_tag="PKY13_05210"
     CDS             complement(4096..4668)
                     /locus_tag="PKY13_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79361.1"
                     /translation="MTSSATPPDPRVPGLEPTFRSLDDADVPWQQVRRQRNADGSEAA
                     VWEKWLAFSPDPQYLSLYAKYDPGMIVRRHGHFSPHLVFVIDGGAWFGDRWCPAGTHI
                     ELPLGAAFGPVRAGDEGAVMFEVMMGDPRSWGDAPEQFDAALAEHGATPLPDVELDYP
                     AWLTDLRTHWVAAGTPETEPESDPLPRGAT"
     gene            4841..5773
                     /locus_tag="PKY13_05215"
     CDS             4841..5773
                     /locus_tag="PKY13_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT79362.1"
                     /translation="MRPAQYLRETLAYAATRRGHDRHDREVATSLTMTADDLDLPPGL
                     ELTWLGVAGVSLAYEGTRILIDPYVTRAPVGDLLRRRVVRPDATLLDRFVPTADAILL
                     GHTHFDHALDAPAIARRDGCTVFGGTSAAHLMGLHGLADRAVTVEPHRVHEVGPFEFR
                     FVPSVHSKLVLGRSVPSAGEITCEHLDGLTSQAYCCGQVWGIHISVAGRTFYHQGSAD
                     LLDDELRAAEVDYFLCGIAGRQVTERYLPRILPRLSPRQVIVMHQDDFLRPLDATPGF
                     AFGVDVAGFPDEVAAVTRDVGVGVLPLLEPLRGS"
     gene            complement(5768..6292)
                     /locus_tag="PKY13_05220"
     CDS             complement(5768..6292)
                     /locus_tag="PKY13_05220"
                     /inference="COORDINATES: protein motif:HMM:NF040142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79363.1"
                     /translation="MSAGVSVLVFSAAWAGFGFASGWLVNQVPLRWLDHDGRLTRLRA
                     FEERGRWYDRNLRVRWWKDRVPEAGGWFRGGVAKDRIRSFRTEDLERLTAETRRAEWV
                     HWANVGFGFTFAAWTEPLVAAVMVVFGVVTHLPFVIIQRYNRARLLYVLTTASRRAQS
                     DPAQPDPAQPAVGS"
     gene            complement(6289..7464)
                     /locus_tag="PKY13_05225"
     CDS             complement(6289..7464)
                     /locus_tag="PKY13_05225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT79364.1"
                     /translation="MWAIRSFAGYELIEMTQFWFVAVGLLVGVALFGRPRRLESTTAG
                     EVTAASGSQVRIVVPARNEAASLANLLDDLAIARPDGADVVVVDDRSTDATGDIARSY
                     SFVTVVRADEPPPGWRGKPWACSVGAARVATLPDPEVLVFLDADVRVRPRAVDEVLAA
                     RRAGGVVSVQPFHRPEGTVEQLSAVFNVVSMMGIGAGGDRPTGMFGPVVCCTAAEYRS
                     VGGHASVRNAVTEDVALARRFIEHNVPLRVRLGGDRISFRMYPTGFAALVEGWTKNMA
                     TGAGTISLARSLAVAWWVTAMVYAAGLAFDAAQGHLDAPGAVTYVLAAATLWWMLRRV
                     GGFRWWVPVLFPVPLAFFVVVFVRSVWFTYVRRSVTWRGRTIDLSEPATHDAAVETP"
     gene            complement(7496..9133)
                     /locus_tag="PKY13_05230"
     CDS             complement(7496..9133)
                     /locus_tag="PKY13_05230"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT79365.1"
                     /translation="MTVTTDDGWLTHVCAPTEMGRLVAAFDWASTPLGPPSTWSAGLR
                     AATSLCLTSRFPMLVVWGPELIQIYNDGYRPMLGDKHANALGRPAGEVWSEVWDVVGP
                     MFESVITTAVPTWEECQSLIVDRHGYAEECFFQYSYSPIYDDDGTVGGVLDVVTETTA
                     AVVAARRLRCLAALGASVSGDGDITDACARAVDTLGRWRPDITGAAIHLTIDGTLTTV
                     ASKREPGAPSLSLDRLAAVESNWRSDRLEGESGLVHQVAEPIGLRGAGVRGVLVLDLD
                     RRRAFDADYQEFTALVAGTVSGAIESAFQRALELGEYRRIADTLQSAMLAPASDIPTV
                     AARYVPALGRLAVGGDWYDVIELTPDTRALVVGDCVGHGLDAAAAMSQLRIAARSMLF
                     DGRDPAGVLDGLHRYAESVEGAECATVVCAIVDRTTSVITYARAGHLPPLVIGAEDSG
                     TRWLDGAGGPPLAAIRDCKYENESAALLTGDRLILFSDGLVERRDETIVDGLERLRVV
                     ADAAKDLPIHDFADHILRTLRPDGAADDIVLVAKLNR"
     gene            complement(9214..10242)
                     /locus_tag="PKY13_05235"
     CDS             complement(9214..10242)
                     /locus_tag="PKY13_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03857 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HOT79366.1"
                     /translation="MATHPELGFYTLAGSPRSPRELLDEVREAEALGLGAAFISERYN
                     IKEACTLSGAAAAVSSDLRIVTGATNHNTRHPLVTASHATTMHFLSEGRFTLGLGRGI
                     AMLQDLYGIPRITTAQMEDFVGLMRRLWKGETIFGHDGPAGSWPMLRLDPEFDEDIPL
                     ALVAFGPNSLALGGRAFDDVILHTFFTDETLQRCVGVVKQAAEEAGRDPDSVRVWSCL
                     ATVGDHLPEPLRLKKTVGRLATYLQGYGDLMVDTNGWDPAVLTAFRSDPVVGSLLGAI
                     DQVATTEQLEHIATLLPDEWLAPAATGTAAQCVATVREQLALGADAVILHGASPTELT
                     PIVHEYSG"
     gene            10329..11123
                     /locus_tag="PKY13_05240"
     CDS             10329..11123
                     /locus_tag="PKY13_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT79367.1"
                     /translation="MELETLEYEERGHVAVVRLNRPEQHNSFNTVMQRELRDTWQAIK
                     VNDDVRAVVLTAAGTKAFCTGIDRSDIPVTEEQFFFDPYTYDDPGKFIGPRSQELWKP
                     VIAAVNGMACGGAFYLLGESDILIASEDASFFDPHVTYGMPAVFEPILMMPRMPFGDL
                     LRMTLTGNDERISAATALRLGLVSEVVPSDELFDTAFALAESIASNPPQAVQASLRTV
                     WAARDLSPQQLQSLGNLFLTKAMTVEALAEGQAIFTSRERGRPRTR"
     gene            11208..12227
                     /locus_tag="PKY13_05245"
     CDS             11208..12227
                     /locus_tag="PKY13_05245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT79368.1"
                     /translation="MRDRFRRTTNQALRSAVAFGAQRVASSERLMSLVYDNMNETNFA
                     GLSHHEEMLSDAVRVDAYHRGIHRNVRPGDVVLDLGTGTGLLAFMASRAGAAKVYAVE
                     HSDFIEVARDIGRRNDFTNVEFVQANSREFTPPEPVDVVLHEQMGDELFNENMLENLL
                     DLRDRVLAPGGRILPSRFRLFMEPVTFRPDMRVRRFHNIELPDGIDLAGTEGAPALAP
                     YESDRFEQFWARPGAVAATVGIPEPVIEFDLATLSSVEALPRRHDIVRTATADAIVDG
                     VCVWFEAQFDDETTLTTSPLAANTSWGNRMFRLDREVHRGETLALRVEMDRLVDASTW
                     SVRDA"
     gene            12253..13428
                     /locus_tag="PKY13_05250"
     CDS             12253..13428
                     /locus_tag="PKY13_05250"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT79369.1"
                     /translation="MTTTPTNGPAMVAAMADLVLAEAEACEQLRTTTAPVVDAMWDTG
                     LMTWFNPSEAGGAEPSFEDMIRTWIDMARLDGSFGWVGIANLPSTAAVAALLPDAGFE
                     AVFTDHDNRVTLGGQFFPNGTGTAVAGGYQVTGAWNFGSATGHSEYIAAGFMPMVDGE
                     MVWAADGIPELRVAIVPRDEVVFTDGWHVQGLKGTGSYDYELTDVFVPGYRTFDLFAR
                     TGRRGAAPTFRMGIMPIVAAGHAAWALGVARSMLDDVRELAMSKVRMGEPATLANTAS
                     FQSGLAHHECAWRAARLLVVQTFADIEATVGGGADLSPTMRADLRAAAVYATDVSREV
                     AQWAHLAAGTTAIREGSRLERAFRDLFTGTQHAFISQKVAIDAAQVWLGLVDDPLGL"
BASE COUNT         2029 a         4672 c         4826 g         1951 t
ORIGIN      
        1 agcgcatgga aacgctgtgc ttcgacctgg ccgaggatcc gggcgaggag cgaaacctcg
       61 tcggcacgcc cgccgaggcg gagctggacg atctgctgcg cacggccctg ctcgagatcg
      121 acgcgccgtc cgatcagctc gaacgcttgg ggctcaccta acagacgcgc gaccttgcca
      181 aggggttttg tgctgcgagg aacgccaaat ttgtcgctcc tcgcagcaca aaaccccaag
      241 gggtcaggcg aggagggcgt cgaggagggg gccggggccg tcgcggagca catcggcggt
      301 ggcgatgccg gtctcggcct gcacggcgcg gaccgcgtcg gcagcttcct ccgaagtgag
      361 gtggccgcag ttgagagcga tggcgcggac cttcgccgcc gggtaggccg tggtcgccgc
      421 agccacgcgt tcgtacaggt cgatcacctc ggggagcggg gggatcgcca cgtcgggcag
      481 cccgtcgagg tgtcgttggc cggcccggtg gacgaggacg agatcggtcg gcatcgaacc
      541 gcgcagcagg gcgatccacg ccgtcgagcc cgggtgcagc gcggagccct ggccctcgac
      601 ccacaacacg tcatggtcgg gtccgtgacg gaggcacagc gactcgatcg cccccgacgc
      661 gaagtcgacg cgcaccgcgt cgagcggcac gccatcgccg ctgatgcaca tgccgatctg
      721 gcccgacgcc acgaaccgtg accgcagacc gcggcggcgg gactcggcgt cgagttcgag
      781 gctcgctgtc atcttgccga tggccatgtc ggtgccgacc gtcaggatgc gccggcagga
      841 cagcgcgcgg gcgcggcccg tgccgctcac gaggccctcg ggctcgcggc gcacgtccca
      901 gatgaacgcg ccgggttgga gcgccgccgc gaactcgggg tcctcggcga accgtccgtg
      961 caggccgttg atcagcgaca tcccgctgcg cagcgccgtc acgatctcgg accggtatcc
     1021 cgccgggagc actccgcccg acgtggcgac ggcgatcacg aggacgtcgg caccgatcgc
     1081 cgccgcggcc tccgcgtcgg cgacgatcgg agcgtcggag tcgatgccgg cgacctcgcg
     1141 gagcgacccg ccggcctcgt cggcatcgat gacgctcgtg acggtgccgg gggagtagcg
     1201 cagcagaccg aaccccatct tgccggtgta ctggcgcagg tggccgtgca tcaggagggc
     1261 gaggcgcgtc gaaccggtga tcctcggtgc gtcgctcgtc gacgccgtcg cgggggttgt
     1321 gggggtcgtc atgcgtcggc gccagtccag cgcaccccga gccccggtcg gtcggacggt
     1381 cgcacgaccc cgtcggtgaa cgtcggcccg tcgaaggggt cgtcgagcag gttcaggtgg
     1441 ctgtcgagat cgaggtggtc gacgaaggga gcgagctgcg cgcctgcacc gatggcgagc
     1501 gacgactcac ccatgcaacc gatcatgacc gacaggccgt gggcccgggc ggtgtggatg
     1561 atgcggaggg cctcgtcgag accaccgcac ttcatgagct tgaggttgac gccgtcgacc
     1621 cggtcggcca gcgcggcgac gtcggcggat gtgcgcaccg actcgtcggc gacgatcggc
     1681 aacgacgaac cgcggtggag cgccggcagc tcgtgttcat ggccctgggg gagcggctgc
     1741 tcgacgagtt cgacaccgcg gtcggcgagc caccggtcca tggcgagcgc tccctccacg
     1801 tcccaaccgc cgttggcgtc gacgcgccac accacgggtc ggtcggcggt cgcccgcgcc
     1861 gattcctgtg ccgccgcgaa catctcccgg tcggcgtcga ggccagccgg ttgaccgagc
     1921 ttgaccttca acaccatcgc ccccgtgcgg tggaggatct cgggcacgac ctcgcggacc
     1981 acgtcgggtg gattgatgcc gacggtgagc gaggtgggga cgatccggcc ggcgtcgccg
     2041 ccgaggagct gccagacggg caggccggcg cgcttgccga gccagtcgta cagcgccatg
     2101 tcgagcgcgg cgcggaccgc gctgccggca tcgtcgtgct ccaggagcgt ggcgatgcgc
     2161 tgccgctcga cgggagcgac cggcgccagg agtggccccc atcgctcgag tgtgacctcc
     2221 gtgtcctccg ccgagtcccc ggtgacgtcg ctcggcgcca tctcgccgat gcccacgatc
     2281 ccgtcgtggc tgacctcgac gaccacgttg gtggaaccgg ccatggtgcc ccggctgatg
     2341 gtcagcgcgt gccgcttggt gaggacgatc cggctcgagg tgacgcgcat cagcggatcg
     2401 taccggcgtc aggtccggtg gagcccgatc gcctcggtca ggccgtcgac gagctcgacg
     2461 acctcaccgc ggccgtgggg caggtcgagg cccgcctccc agcggtggag ctcggtgttg
     2521 atcgccacgg cgaggtcgcg gcccccgtcg gtcagctcga tcacgacgcg ccgaccgtcc
     2581 tcggggtccg gtgaacggcg caaccacccc gcctgttcga ggcggtcgag cgtcagcgtc
     2641 atgcccccgg tggtccgtcc gagcgtgtcg gcgatcgccg tcggcgcgct ccgcccgtcg
     2701 ggcgcgccgc ggaccacccc gagcacgagg tagtccgcca tcgtcaggcc gtgggcgccg
     2761 gcgatgcctt cgagcacgcg ggtgaccgcg aggttgaggc ggacgatgcc gccgagcagc
     2821 ccgtcgtcgg ggggagtggc cacggcccgt cgcgtcagac gctgatgccg aagcgctcga
     2881 ggccgtagca acggatggcg ttgccgcgga ggaactcgta gcactcctgc tcgttcatgc
     2941 cggcgccggc gcagagccgg tgcgcgaccg agcgcgagtg aggccacgtg ccgtcggtgt
     3001 gggggtagtc ggtctcgaag aggatgtgct cgaggccgac ggcgtcgcgg ctcttgaggc
     3061 cgtgctggtc gtcgaacacg cagccccaca cccggccctt cacgatctcg ctcggcatgc
     3121 ggtcgagctg caccccggcg gcagcgccct cggtggcgac gatgtccatg cgctcgatca
     3181 ggtagggcat ccagccgatc tggctctcgg cgaacgcgat cttgagcgtg gggaagcggg
     3241 acagcgtgcc cgagaagatc cagtcggtga gtgacccctg ggcgttctgc gaactgagcg
     3301 ccatcgacac accgaggggc gcgtcggggc tggtggtcgg catggtcgac gacgagccga
     3361 tgtgcatgct gacggtggtg tcgctctcct ggcaggcgtc ccacaggacg tcccactcgc
     3421 cgctgtgcat ggaggcgaag ccgagcttcg aggggttctc ggagaaggcg atcgcgtagc
     3481 tgcccttggc ggcgcaacga cggacctcgg cggcggcgag caccgggtcc cacaggggca
     3541 cgagggtgag cgggatgaga cggccgttgg cgtcggcgcc gcaccagtcg tcgatcatcc
     3601 agtcgttgta gatctgcagg cagagcaggg cgaagtcctt gtcgtcgcgc tcggcgaagc
     3661 cctggccggc gaaccggggg aacacattgg ggtagttgat ggcggcctcg acgtggttgg
     3721 ccgtcatgtc ctcgaggcgc gccgtcttgt cgtaggtgcc gggacggaag tcctcgtaga
     3781 tcgccgggat gttgatctgc tcctcgtcgg ggtagccgac ggcggcgtgc aggaggccgg
     3841 tgggcacaac gaggtcgtcg aagagccaca ggtcgcacag cttgccgtcc tcggccccac
     3901 gggtgaaccc gtagtgcccg cccttgaact cgagcgagac cttctcgcgc acgacgcggg
     3961 ggccgcggtc gcgaaggctg ggcggaagct gctcctgcca cagcgtccgg ggttcgagga
     4021 tgtggtcgtc gaccgagatg atcttcggca ggtcctcggc ggccggtgtc acggaggtgg
     4081 ggatgtcggt ggcggtcatg tcgctcctcg tggaagtggg tcggattcgg gttcggtctc
     4141 gggggtgcct gccgcgaccc agtgggtgcg caggtcggtg agccaggcgg ggtagtcgag
     4201 ctcgacgtcg ggcaggggtg tggcgccgtg ctcggcgagg gcggcatcga actgctcggg
     4261 agcgtcgccc cacgatcggg ggtcgcccat catgacctcg aacatcacgg cgccctcgtc
     4321 gccggcgcgc accggcccga acgcggcgcc gagcggtagc tcgatgtggg tgccggccgg
     4381 gcaccagcga tcaccgaacc aggcgccgcc gtcgatgacg aagacgaggt gggggctgaa
     4441 gtggccgtgg cgacgcacga tcatgccggg gtcgtatttg gcgtacagcg acaggtactg
     4501 ggggtcgggc gagaacgcga gccatttctc ccagacggcg gcctcggagc cgtcggcgtt
     4561 gcgctgtcgg cgcacctgct gccagggcac gtcggcgtcg tcgagcgagc ggaaggtggg
     4621 ctcgagaccc gggacccggg ggtcaggtgg tgtggctgac gacgtcacgg agatagcttt
     4681 gcacaaagct acctctgcgg caatcatccc gatgggggag accgagcggc cgtcggccgg
     4741 ggcgcagcgg tcgtgcccgg gggactgccc gggagactgc ccggtagcgg gcgcatccct
     4801 gcgagactca tcgctcgcac ctcgggagga gtcgcgagcg atgagaccag cgcagtacct
     4861 gcgggagacc ctggcctatg cggcgacgcg gcgcggccac gaccgtcacg accgcgaggt
     4921 cgcgacgtcg ctcacgatga cggcggacga cctcgacctc ccgcccggac tcgagctcac
     4981 ctggctcggc gtcgccggag tctcgctcgc ctacgaaggc acgcggatcc tgatcgaccc
     5041 ctatgtcacc cgggcgccgg tcggcgatct gttgcgccgt cgggtcgtcc ggcccgacgc
     5101 caccttgctc gaccgcttcg tgccgaccgc cgacgccatc ctcctcgggc acacccactt
     5161 cgaccacgcg ctcgacgccc cggccatcgc ccgccgcgac gggtgcacgg tgttcggcgg
     5221 cacctccgcc gcccacctca tgggcctgca cggtctggcc gaccgtgcgg tgaccgtcga
     5281 accgcaccgg gtccacgagg tcgggccgtt cgagttccgc ttcgtcccga gcgtgcactc
     5341 caagctcgtg ttgggccgct cggtgccgag cgccggtgag atcacctgtg agcacctcga
     5401 cgggctcacc tcccaggcgt actgctgtgg ccaggtctgg gggatccaca tctcggtcgc
     5461 cgggcgaacc ttctaccacc agggttccgc cgacctgctc gacgacgagt tgcgggccgc
     5521 cgaggtcgac tacttcctgt gcggcatcgc cggtcggcag gtgacggagc ggtacctgcc
     5581 ccggatcctc ccgcgactca gcccccgcca ggtgatcgtc atgcaccagg acgacttcct
     5641 ccgccccctc gacgccacgc cggggttcgc gttcggtgtc gacgtcgccg ggttccccga
     5701 cgaggtcgca gcggtcaccc gcgacgtcgg ggtgggcgtc ctgccgctcc tggagccgtt
     5761 gcgcggctca tgagccgacg gcgggctgtg ccgggtcggg ttgtgccggg tcggactggg
     5821 cccggcgaga cgccgtggtg agcacgtaga gcaaccgggc gcggttgtag cgctggatga
     5881 tcacgaacgg cagatgggtc acgacgccga acacgaccat cacggcggcc acgagcggct
     5941 cggtccaggc ggcgaacgtg aacccgaacc cgacgttcgc ccagtgcacc cactcggcgc
     6001 ggcgggtctc ggccgtgagc cgctcgaggt cctcggtacg gaacgaacgg atccggtcct
     6061 tggcaacacc gccgcggaac cacccgcccg cctcgggaac gcggtccttc caccaccgca
     6121 ccctgaggtt ccggtcgtac catcgacccc gctcctcgaa cgcccggagg cgggtcagtc
     6181 ggccatcgtg gtcgagccac cgcagcggca cctggttgac caaccagccc gatgcgaaac
     6241 cgaagccggc ccacgccgcc gagaacacga gcaccgacac gccggcgctc atggtgtctc
     6301 gaccgccgcg tcgtgcgtcg ccggttcgct gaggtcgatg gttcggcccc gccatgtgac
     6361 cgagcggcgg acgtaggtga accacaccga ccggacgaac accaccacga agaacgccag
     6421 cggtacgggg aagaggacgg gcacccacca gcggaacccg cccacgcggc gcagcatcca
     6481 ccacagggtg gcggcggcga ggacgtaggt gaccgcaccg ggcgcatcga ggtgcccctg
     6541 ggcggcgtcg aacgccaggc cggccgcgta caccatcgcc gtcacccacc aggccacggc
     6601 gagcgaacgc gccagggaga tcgttccggc gccggtcgcc atgttcttcg tccagccctc
     6661 gacgagcgcc gcgaacccgg tgggatacat gcggaacgag atccggtcgc cgccgagacg
     6721 aacccgaagc ggcacgttgt gctcgatgaa tcgtcgggcc agcgccacgt cctcggtgac
     6781 ggcgttgcgg accgacgcgt gaccgcccac cgatcggtac tccgcggccg tgcagcacac
     6841 gacgggcccg aacatccccg tcggccggtc accgccggcc ccgatgccca tcatcgagac
     6901 gacattgaag acggcgctca gctgctcgac cgtgccctcg gggcggtgga acggctggac
     6961 ggacacgacc ccgccggcac gtcgcgccgc cagcacctcg tcgacggcgc gggggcggac
     7021 ccgcacgtcg gcgtcgagga acaccagcac ctcggggtcg ggcagcgtcg caacccttgc
     7081 ggcaccgacc gaacaggccc acggttttcc tcgccaaccc ggcggtggct cgtcggcgcg
     7141 caccacggtg acgaaggagt acgaccgggc gatgtcaccg gtcgcgtcgg tggagcgatc
     7201 gtcgacgacc acgacgtcgg cgccgtcggg gcgggcgatc gcgaggtcgt cgagcagatt
     7261 cgccaacgag gcggcctcgt tgcgcgccgg gacgacgatc cggacctgcg atccggacgc
     7321 agcggtgacc tcgccagccg tcgtcgattc gagccggcgg ggccgaccga acagcgcgac
     7381 gccgaccagc agccctaccg cgacgaacca gaactgtgtc atctcgatca gttcgtaccc
     7441 cgcgaacgac cggattgccc acatttcgga attgttttcg gtgacgtcct gtcggtcacc
     7501 ggttcaactt ggcgacgagc acgatgtcat cggcggcccc gtcggggcgc agcgttcgga
     7561 ggatgtgatc ggcgaagtcg tggatcggca ggtccttcgc ggcgtcggcg accacccgga
     7621 gccgctcgag gccgtcgacg atcgtctcgt cgcgccgttc gaccaggccg tcggagaaga
     7681 ggatcagccg atcgccggtg aggagcgctg ccgattcgtt ctcgtacttg cagtcgcgga
     7741 tcgccgcgag cgggggccca ccggcgccgt cgagccagcg ggtcccggag tcctccgctc
     7801 cgatgacgag gggcgggagg tggccggcgc gggcataggt gatgacactg gtggtccggt
     7861 cgacgatggc gcacaccacc gtggcgcact cggcgccctc gacggactcg gcatagcggt
     7921 gcaggccgtc gagcacgccg gctgggtccc ggccgtcgaa cagcatcgat cgcgctgcga
     7981 tgcgcaactg tgacatcgct gcggctgcgt cgagaccgtg cccgacacag tcgcccacga
     8041 cgagcgccct ggtgtccggg gtgagctcga tcacgtcgta ccagtcgcca ccgactgcga
     8101 gtcgcccgag cgccgggacg taccgcgcgg cgacggtcgg gatgtcgctc gccggtgcga
     8161 gcatcgcgct ctgcaacgtg tcggcgatgc ggcggtactc acccagttcg agcgcccgtt
     8221 ggaacgccga ctcgatcgcc ccgctgacgg tgccggccac gagggccgtg aactcctggt
     8281 agtcggcgtc gaaggcgcgg cgtcgatcca ggtcgaggac gagcacgccg cggacccccg
     8341 ctccccgcag cccgatcggt tcggcaacct ggtggaccag ccccgactcg ccctccaacc
     8401 ggtcggaccg ccagttgctc tcgacggccg ccaggcggtc gagcgacagg ctcggcgctc
     8461 ccggctcgcg tttcgacgcg accgtggtga gcgtcccgtc gatcgtgaga tggatcgccg
     8521 ccccggtgat gtcgggtcgc caccggccga gcgtgtcgac cgcccgggcg cacgcgtcgg
     8581 tgatgtcgcc gtcgcccgac acgctcgccc cgagcgcggc aaggcagcgg aggcgccgtg
     8641 ccgcgaccac ggcagcggtt gtctcggtga cgacgtcgag gacgccgccg acggttccgt
     8701 cgtcgtcgta gatggggctg taggagtact ggaagaagca ctcctcggcg tacccgtgcc
     8761 gatcgacgat cagcgactgg cactcctccc acgtcggcac ggccgtggtg atgaccgatt
     8821 cgaacatcgg cccgaccacg tcccacacct ccgaccacac ttcgccggca gggcgtccga
     8881 gggcgttggc gtgcttgtcg ccgagcatcg gtcggtagcc gtcgttgtag atctggatga
     8941 gctcggggcc ccacacgacg agcatgggga agcgcgaggt caggcacaac gaggtcgcgg
     9001 cgcgaagacc ggcgctccac gtcgacggcg ggccgagtgg tgtcgaggcc cagtcgaacg
     9061 cggcgacgag ccgacccatc tcggtcggtg cgcacacgtg cgtgagccaa ccgtcgtccg
     9121 tcgtgaccgt cacggtgcga acctacccgg gtccgcgggc cgagcgaagg gggagaatgg
     9181 gggattctcc ccggcggcgg gcgggtggtc gtcctagccg ctgtactcgt ggacgatcgg
     9241 ggtgagttcg gtcgggctcg cgccgtggag gatcacggcg tcggcgccga gcgcgagctg
     9301 ttcgcgaacc gtcgccacgc actgcgcagc ggtgcccgtc gcagcggggg cgagccactc
     9361 gtcggggagc agcgtggcga tgtgttcgag ctgttcggtc gtcgcgacct ggtcgatcgc
     9421 cccgaggagg ctgccgacga cggggtcgga acggaacgcg gtgagcaccg ccgggtccca
     9481 cccgttggtg tcgaccatga ggtcgccgta gccctgcaga taggtggcaa gacgtccgac
     9541 ggtcttcttg agtcgcagcg gctcgggcag gtggtcgccg acggtggcga ggcacgacca
     9601 gacgcgcacc gagtcggggt cgcggccggc ctcctcggcc gcctgcttca cgacgccgac
     9661 gcagcgttgg agggtctcgt cggtgaagaa cgtgtggagg atgacgtcgt cgaacgcccg
     9721 accgccgagc gccaggctgt tcggcccgaa cgcaacgagg gcgaggggga tgtcctcgtc
     9781 gaactcgggg tcgagccgca acatgggcca cgatccggcc ggcccgtcgt ggccgaagat
     9841 cgtctcgccc ttccagaggc ggcgcatcag gccgacgaag tcctccatct gcgcggtcgt
     9901 gatacgcggg atgccgtaga ggtcctgcaa catcgcgatg ccccgaccca gcccgagcgt
     9961 gaatcggcct tcggacagga agtgcatggt ggtggcgtgc gatgccgtca cgagcgggtg
    10021 ccgggtgttg tggttggtgg cgccggtcac gatccgaagg tcgctcgaca ccgcggcggc
    10081 ggcgccgctc aacgtgcacg cctccttgat gttgtagcgc tccgagatga acgcggcgcc
    10141 gaggccgagc gcttcggcct cgcgtacctc gtcgagcagt tcccgcggag agcggggcga
    10201 tccggcgagc gtgtagaagc cgagttcagg gtgggtggcc atggcgcccg aggctactgt
    10261 cgggcgggtc ggcgccgtcg tggcgaccgt gacatcgacg agccgtcatc ggcatcagga
    10321 gccccacggt ggaactggaa accctcgagt acgaagaacg cggccacgtt gcggttgtgc
    10381 gcctcaaccg gcccgagcag cacaactcgt tcaacaccgt catgcagcgc gagctgcgcg
    10441 atacctggca ggcgatcaag gtcaacgacg acgttcgggc cgtggtgctc accgccgcgg
    10501 gcaccaaggc gttctgcacc ggcatcgacc gcagcgacat cccggtgacc gaagagcagt
    10561 tcttcttcga cccctacacc tacgacgacc ccggcaagtt catcggcccg cgcagccaag
    10621 agctgtggaa gcccgtgatc gctgcggtca acggcatggc gtgcggcggt gccttctacc
    10681 tgctcggcga gtccgacatc ctcatcgcgt cggaggacgc atcgttcttc gacccgcacg
    10741 tgacctacgg catgcccgcc gtgttcgaac cgatcctgat gatgccgcgc atgccgttcg
    10801 gcgatctgct gcgcatgaca ctcaccggca acgacgagcg gatcagcgcc gcgaccgccc
    10861 tgcggttggg cctcgtgagc gaggtcgtgc cctccgacga gctgttcgac acggcgttcg
    10921 cgctcgccga gtcgatcgcg tcgaacccgc cgcaggcggt gcaggcctcg ttgcggacgg
    10981 tgtgggccgc ccgcgacctc agcccccagc agctgcagtc gctcggcaac ctgttcctta
    11041 ccaaggccat gaccgtcgag gcgctcgccg aggggcaggc gatcttcacg tcgcgggagc
    11101 ggggccgccc ccgcacgcgc tgacgcgatc caggcccagc cgggcacagg tgcccgactg
    11161 agcctggatc cggccggccg gcgcccatcg gccagactgg cgtcatcatg cgagaccgct
    11221 tccgacgtac caccaaccag gcgctccgat cggcggtcgc cttcggagcg caacgcgtcg
    11281 cgtcgagcga gcggctcatg agcctcgtgt acgacaacat gaacgagacg aacttcgccg
    11341 gcctctccca ccacgaggag atgctgagcg acgccgtccg cgtcgacgcc taccaccggg
    11401 gcatccaccg caacgtgcgc cccggcgacg tggtgctcga cctcgggacc ggcacgggcc
    11461 tcctggcgtt catggccagc cgggccggtg ccgccaaggt gtacgccgtc gaacactccg
    11521 acttcatcga ggtcgcccgc gacatcggcc gccgcaacga cttcaccaac gtcgagttcg
    11581 tgcaggccaa cagccgtgag ttcaccccgc ccgagccggt cgacgtggtg ttgcacgagc
    11641 agatgggcga cgagctcttc aacgagaaca tgctcgagaa cctgctcgac ctgcgcgacc
    11701 gggtgctcgc ccccggcggt cgcatcctgc cctcccggtt ccggctgttc atggaacccg
    11761 tgacgttccg tcccgacatg cgggtccgtc gcttccacaa catcgaactg cccgacggca
    11821 tcgacctcgc cggcaccgag ggcgcgccgg cgctggcgcc ctacgagtcg gaccggttcg
    11881 agcagttctg ggcgcgcccc ggagccgtcg ccgccaccgt ggggataccc gagcccgtca
    11941 tcgagttcga cctggccacg ctcagttccg tcgaggcgtt gccccgtcgc cacgacatcg
    12001 ttcggaccgc aacggccgac gcgatcgtcg acggggtgtg cgtctggttc gaggcgcagt
    12061 tcgacgacga gacgaccctc acgacctcgc ccctcgccgc gaacaccagc tggggcaacc
    12121 gcatgttccg cctcgaccgc gaggtccacc gtggcgagac gttggcgctg cgcgtcgaga
    12181 tggaccggct cgtcgacgcg tcgacgtggt cggtgcgcga cgcttgacgc cgcacaggaa
    12241 caggggagac acatgaccac aacaccgacg aacgggccgg cgatggtggc ggcgatggcc
    12301 gacctcgtgc tggccgaggc cgaggcgtgc gagcagctcc gcaccacaac ggcccccgtg
    12361 gtcgacgcga tgtgggacac cggcctgatg acgtggttca acccgtcgga ggccggcggc
    12421 gccgagccgt ccttcgagga catgatccgc acctggatcg acatggcccg gctcgacggc
    12481 tcgttcggtt gggtcggcat cgcgaacctg ccctccaccg cagcggtcgc agcactcctg
    12541 cccgacgccg gcttcgaggc ggtgttcacc gaccacgaca atcgggtgac cctcggcggc
    12601 cagttcttcc cgaacggcac cggcacggcc gtcgccggtg gctaccaggt gacgggagca
    12661 tggaacttcg gctcggccac cggccacagc gagtacatcg ccgcagggtt catgccgatg
    12721 gtcgacggcg agatggtgtg ggccgccgat ggcatcccgg agctgcgggt ggcgatcgtg
    12781 ccacgcgacg aggtcgtgtt caccgacggc tggcacgtgc agggcctgaa gggcaccggg
    12841 tcctacgact acgagctgac cgacgtgttc gttcccgggt accgcacctt cgacctgttc
    12901 gcccggaccg gccgccgcgg cgcggcgccg acgttccgga tgggcatcat gccgatcgtc
    12961 gccgccggcc acgccgcgtg ggcgctcggt gtcgctcgaa gcatgctcga cgacgtgcgc
    13021 gagctggcga tgagcaaggt gcgcatgggc gagccggcga ccttggcgaa cacggcctcg
    13081 ttccaatccg gtctcgccca tcacgagtgc gcgtggcggg cggcccgcct gctcgtcgtg
    13141 cagacgttcg ccgacatcga agcaacggtc ggcgggggcg ccgacctgtc cccgacgatg
    13201 cgcgccgacc tccgggccgc cgccgtctac gccaccgacg tgtcccggga ggtcgcccag
    13261 tgggcgcacc tcgccgccgg cacgaccgcg atccgtgaag gcagccgcct cgagcgtgcc
    13321 ttccgcgacc tcttcaccgg cacccagcac gcgttcatca gccagaaggt ggcgatcgac
    13381 gcggcgcagg tgtggctcgg cctcgtggac gatcccctcg gcctctgacc cgaatccctc
    13441 cagaaccctg ggggtgcagg tgcaccggca cgttcgcg
//
LOCUS       DAOSOA010000052        13444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4610_length_13444_cov_5.870491, whole genome shotgun sequence.
ACCESSION   DAOSOA010000052 DAOSOA010000000
VERSION     DAOSOA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4610_length_13444_cov_5.870491"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PKY13_05255"
     CDS             <1..874
                     /locus_tag="PKY13_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HOT79370.1"
                     /translation="GAMYCMSQRGEHIWEGVSSATTRSRPIINTRDEPHADAERYRRL
                     HVIVGDTNMGEYPTFLKVGAASIILRMLEDPGVVLRDMTLENPIRAIREISHDLTCRH
                     RVRLANGREASAIEIQSEYLNRALRYAQTHDFSPQEKQALSMWEHVMSKLETDPFSLD
                     REVDWVTKHNLIEHYRERHQLPLSSPEASLVDLQYHDISRRRSVFYKLQKRGLVERMV
                     TDRDIADAMEIAPQTTRARLRGEFIKKAKERKRDYTVDWVHLKLNDQAQRTVLCKDPF
                     KSHDERVDKLIESL"
     gene            885..1904
                     /locus_tag="PKY13_05260"
     CDS             885..1904
                     /locus_tag="PKY13_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HOT79371.1"
                     /translation="MPTFRSGEVTGIISERPGLQRVRVRLDGAADESRAYSLTQLTGA
                     VNVGDAVVCNTTAVELGLGTGGWHVVHWNLSRTEFVAPGPDHVMKLRYTSLQFDAGTS
                     ELEYPDLPTTIDGVPVVVCQVHSQVGVVVATIAALRPGTRVAYVMTDGASLPIALSDL
                     VEDLRSRELLHTTVTAGHAFGGDLEAVTVASGLVLAHHVADAELIVVGMGPGVVGTGT
                     KFGTTSVEAAAALDVVDALGGIPVLCVRASDGDARERHQGVSHHTLTAIELTRSAPVV
                     AAVPHEVLTLEGVVVAEVDPPDVRDILGAAGLRITTMGRDIDADPLFFTAAGAAAAAA
                     VTFLS"
     gene            2014..2952
                     /locus_tag="PKY13_05265"
     CDS             2014..2952
                     /locus_tag="PKY13_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT79372.1"
                     /translation="MTTKLERLLNLTALLLDTERPVGIEQIGRVIEGYPPDPASFRRT
                     FERDKDDLREMGIPVEVINTDVHDKTALGYRILPERYYLADPGLEPDEMAALKLAMGA
                     IRLDGIATQETLRKLGDASGDSAQPELASVPTPPVIGDLHAAINEHRLVQFAYNGEQR
                     TVEPQRLEFHKGRWYLRGFDRLREDRRSFRIDRVEGSITAGEPGSFEPSGHDAGPAGF
                     RFDPWRHGDDAPVTAVLRIDATQAAFSRSLVQEEAVWTDLDDGSVTLEVEVTNRRAFR
                     TFVLGLLEHAEIVSPPELREDLVEWLEATIASRGAV"
     gene            2949..3929
                     /locus_tag="PKY13_05270"
     CDS             2949..3929
                     /locus_tag="PKY13_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT79373.1"
                     /translation="MKPRVTASDRFRRIVAIVPWIAERGGPRIDEVCAQFDISREDLL
                     ADLDVIFMVGIPPYTPDELIDVIIDDDRVWVTLGRYFTRPLRLTAQEALAVLAAGSGL
                     MATTGADPEGPLARALTKLQRVVGIGEPGSLEVSLGGADSGTLALLQQATTERRQIEI
                     DYYSYSTDESRVRTIDPYRVYATDGNWYVIGWCHSADAERLFRVDRIRSAHLTDGSFE
                     LPAELPAATSYSGSGADPRIAIEVGPADRWIAEHYPTDRFEELPDGRYRAVLAVGGDA
                     WLERLLLRLGPEATVLDLPDGEVDGPTMSSDAARALTEPMAQSIIGLYRS"
     gene            4000..5445
                     /gene="lepB"
                     /locus_tag="PKY13_05275"
     CDS             4000..5445
                     /gene="lepB"
                     /locus_tag="PKY13_05275"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT79374.1"
                     /translation="MPVSSSTEPLDDRLTDMGRALANSLLGDVTVARPIREPAEPEVP
                     LSRRARREQRERAAAGRGERHEFPPPTAAAPSAPAAPARPAAPSAQAAPASSAPPSAP
                     VARAGEGAALVRPGGVPGVRDAAAPPVAPDGTQFQIARPRRRSAPDPAAPSSSAPTPP
                     RPAAPAAPPVAPQRRPAPPTQRPDSPRPQRPQAPAAPAAASAPATAPAPAPAPPAAAR
                     AGRVDARPDEQPPLRPRLRNLAPDPYAALPWPADEAVSYHDDIRRAPESRPGSRIRDL
                     DLTVARAEPAEQPEDPTEESPRGGLVRTVVEWAMVLGTAAVVALLLQFFVVQLYEIPS
                     ESMYPTLQNNDQVLVNKLAYAVGGGVERGDVIVFKRPESERTDDPDQPPQLIKRVIGL
                     PGDVVEARGGVVYIDGKPLEETGTGHYLDPSVVTNNLPQPVTVPRGHVFVMGDNRERS
                     HDSRYFGAIPESDIIGRAVVIAWPFSRWGSL"
     gene            5448..6101
                     /gene="lepB"
                     /locus_tag="PKY13_05280"
     CDS             5448..6101
                     /gene="lepB"
                     /locus_tag="PKY13_05280"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT79375.1"
                     /translation="MFDQTNTTGVPPEYRPLLDPPAPPVTAEPVPSERVLELNAEPAA
                     NPKGTAMGWALTIAVAVVLTIVVKTFILQAYSIPSESMVPTLNVGDRVVVFRLNTDPA
                     RGDIVVFDRPANDPKTSPSDPDVLIKRVIGLPGDVVESRDGEVWVNGKQLVEDYLPDG
                     TTTIIDTPITVPSGKLLVLGDNRSRSFDGRIFGPIDKDLVVGRAIARIWPLSRLGGL"
     gene            complement(6113..6847)
                     /locus_tag="PKY13_05285"
     CDS             complement(6113..6847)
                     /locus_tag="PKY13_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT79376.1"
                     /translation="MIPRQEPLAGPAILFASGGARGHTDENTPESFRLAVRLGATGIE
                     TDLQVTADGVPVLRRDARLGGLRRRRIADTSSADLPDDVVTLAGFYEEHGATLDLLVH
                     VRTVDAVEEAIALATRHRALDRLWLAASDRAALLAWRARSELVRLVDTTTLNDLGAGA
                     ERHAAGLREDRIDAKLLPQADWNGGRAALFHRFGRRCFGTDAHHDRMILGLLHIGMDG
                     VISSYPDRLVDAAAAIARPDAPQFLE"
     gene            7010..7342
                     /locus_tag="PKY13_05290"
     CDS             7010..7342
                     /locus_tag="PKY13_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79377.1"
                     /translation="MALIRATCSECGDVELRSRDLQVRICGDTGDGTYLFRCPVCRMT
                     EVKNADDQVVDILAAAGVKCVEWRLPAEMTERPTGEPITHDDVLDFHEILGGDDWFTT
                     LSEMTRHG"
     gene            7398..8045
                     /locus_tag="PKY13_05295"
     CDS             7398..8045
                     /locus_tag="PKY13_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOT79378.1"
                     /translation="MLIGGLLGILDSLTGGEVIMILVAALLVLGPERLPETARTMGQW
                     LAKLKSMTGNLQSEVREVLDDPSMKPIRDLGEFAAAPRKKIVEFATAAEAEANLAKQV
                     AAEAEAAAAEAEAAAAKARAAADEAAAEAEEAEQVAEDAAELVEETPPDEPAPGEPGA
                     GREASIWADLEDEPLEDEPVVAEPLEDAPLEDGTPWSSNHGAIQASVDRGASEPT"
     gene            8111..8857
                     /gene="tatC"
                     /locus_tag="PKY13_05300"
     CDS             8111..8857
                     /gene="tatC"
                     /locus_tag="PKY13_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOT79379.1"
                     /translation="MSLMDHLRELRNRIFKSALAVFVAGIGVYFVYHRLYDVLVEPYC
                     EAIKNTAQESCKLLYTDPVSPFAMQLRVSAYVGILIALPVIFWQLWRFVAPGLYQKEK
                     RYAIAFVASSVVLFLLGAALAWYSLPKIFEWLVDQAGDALVQTKVDEYMSLLALMVFA
                     FGLSFEFPLILLALQLMGVVSPDTLAGVRRHAIVGIVAVVAIVTPGGDPVSLFALSIP
                     LCIFYEASIWIARLMLGRRAKNREATDTAA"
     gene            8854..11559
                     /locus_tag="PKY13_05305"
     CDS             8854..11559
                     /locus_tag="PKY13_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT79380.1"
                     /translation="MTVSPSDSAPFTLDRFQLEAIAEFDAGRSVLVAAPTGSGKTVVA
                     DAAIDIALNAGGKTFYTTPIKALSNQKYSDLVARLGTERVGLLTGDTAINGDAPVVVM
                     TTEVLRNMLYSGSAALDNLHVVVLDEVHYLQDAYRGPVWEEVIIGLPPNVRLVCLSAT
                     VSNAGELGEWIETVRGPTSTVIEHERPVELENLYLVGDRSSDRDHLIPVLVDGRPNPE
                     GYRFDGDASGGRGRHTGAPRDRRRRFVTPRRLETIERLAEEDLLPVIYFIFSRNACDD
                     ALAIARDSGLRFTDAEERIRIREILEGSVSMLSDSDLDVLGYDMWAAALEQGIASHHA
                     GLIPAFKEAVERCFVEGLVKVVFATETLALGINMPARSVVIEKLTKYNGDTHEFLTPA
                     QFTQLTGRAGRRGIDDEGFSVVLWSPFVSFAQVAGLAGSREFPLTSSFRPTYNMAANL
                     VRRYEKTEAMSLLGQSFAQFQADRAVVTLERRLARDRERLAVIDEQVGCERGDVAEYA
                     ALDERVNSQRTNRPDGRIAIERSVSLLRPGDVIAPRLREDTTARLHLHPHKPVRLAVI
                     SVAYRGKGSLRVRTVDERGDAVQLVVADFREPSRAVDEIELPTPYAPHDPAFLDETAS
                     RLRAARATVSTAPSGASRWDRLVAELESHPVHDCPDRDDHLAAYRERSGLERDVARLS
                     KQLHQRSSSVVRRFEAVADLLGHLGFLDGWALTAPGERLARIYHERDLLVALALDDGV
                     FDGLDAPGVAALVAAVVFEERRPDGSEPVYPNRNLRQRFGRLERLADKIARAERERGL
                     PQTRPPDPGFMAVCHGWASGRDLGGLIEGEMSGGDFVRTTKMIIDVLGQIAEIAPDLG
                     TRSRAAAAVEAIRRGVVADFGPSEALPKAAEELPKAAEDPPKAGT"
     gene            11556..12152
                     /locus_tag="PKY13_05310"
     CDS             11556..12152
                     /locus_tag="PKY13_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79381.1"
                     /translation="MIRKGEVWGRPVDLDGTEPEASSDAEVARLMSRPLDSPVVLRGG
                     DLYHSLGGRASSPVDLDGPVMALPIDLVAVRVDGVDRVAAAHVVARRRWWSGEFAVAM
                     NGTHLGEWNLGPKAHPNDGLIDVTTGRLDVRDRWEARRRAHTGTHLPHPALSTSRVTS
                     TVWTFDRPTPVYVDGVAVGSARRVELEVQPDRGSVVVG"
     gene            12234..13220
                     /locus_tag="PKY13_05315"
     CDS             12234..13220
                     /locus_tag="PKY13_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT79382.1"
                     /translation="MQSWFLSESPGEYQWGEVPTPEPGVGEIRVRLVASGVNHIDHWQ
                     TRGLPKPKSFPHVPGSDGAGVVDAIGDGVTRFSVGDEVAINAAQTNAAAIEKFGIDSV
                     FDPSLQLMGEHRWGCHGEQVVIPDYGAELRPAGRSWEECAAYPVALTTAWRMFRRARL
                     QAGEIVLVTGIGGGVATAAQMLAQHMGARVFTTSRDAAKRARSIELGAEDSFDSHERY
                     PIKADVIVDSIGPATWNQTVAALKAGGRLATCGGTSGSDVQLSLPRLFFKQHEIVGST
                     LGSQEEFAYVTQLMAEGLPVVIDEVFAWDDYPRAVERIRKADQLGKLILRHP"
BASE COUNT         2026 a         4803 c         4592 g         2023 t
ORIGIN      
        1 cggcgccatg tactgcatga gccagcgcgg cgagcacatc tgggaagggg tcagctctgc
       61 cacgacccgc tcgcggccca tcatcaacac ccgcgacgag ccgcacgccg acgccgagcg
      121 ctaccgccgc ctgcacgtga tcgtcggcga cacgaacatg ggggagtacc ccacgttcct
      181 caaggtcggc gcagcctcga tcatcctgcg catgctcgag gatcccggtg tggtgctgcg
      241 ggacatgacc ctcgagaacc cgatccgggc gatccgcgag atcagccacg acctcacgtg
      301 tcggcaccgc gtgcgccttg ccaacgggcg ggaggcgtcg gccatcgaga tccagtccga
      361 gtacctcaac cgagcgctcc gctacgccca gacccacgat ttctcccccc aggagaagca
      421 ggcgctgagc atgtgggagc acgtgatgag caagctcgag accgatccgt tctccctcga
      481 ccgcgaggtc gactgggtga ccaagcacaa cctcatcgag cactaccgcg agcggcacca
      541 gctgccgttg tcgtcgccgg aggcgtcgct cgtcgacctg cagtaccacg acatcagccg
      601 tcgccgctcc gtgttctaca agctgcagaa gcgtggtctc gtcgagcgca tggtcaccga
      661 ccgcgacatc gccgatgcca tggagatcgc accgcagacg acccgggccc gcctccgtgg
      721 cgagttcatc aagaaggcca aggaacgcaa gcgcgactac acggtcgact gggtacacct
      781 gaagctcaac gaccaggccc agcgcacggt gctctgcaag gacccgttca aatcgcacga
      841 cgaacgcgtc gacaagctca tcgagagcct ctaagcccgt tcgaatgccg acgttccgct
      901 ccggtgaggt caccgggatc atctccgagc gcccgggtct ccagcgggtg cgggtacgac
      961 tcgacggtgc cgccgacgag tcgcgggcgt attccctcac gcagctgacc ggcgccgtga
     1021 acgtcggtga cgcggtggtg tgcaacacca ccgcggtcga actcggcctc ggcaccggcg
     1081 gctggcacgt cgtgcactgg aacctcagcc gcaccgagtt cgtcgctccg ggtcccgatc
     1141 acgtcatgaa gctccggtac acgagcctgc agttcgacgc cggaaccagt gagctggagt
     1201 acccggacct cccgacgacg atcgacggtg ttccggtcgt ggtgtgccag gtgcacagcc
     1261 aggtcggtgt ggtcgtggcg acgatcgccg cgctgcgccc cggcacccgc gttgcgtacg
     1321 tcatgaccga tggggcgtcg ctcccgatcg cgctgagcga cctcgtcgag gatctccgct
     1381 cgcgcgagct gctccacacc acggtcacgg ccggtcatgc cttcggcggc gacctcgagg
     1441 ccgtcacggt ggcgtcagga ttggtgctcg cccatcacgt cgccgacgcc gagctcatcg
     1501 tcgtcggcat gggtcccggg gtcgtcggca ccgggacgaa gttcggcacg acctcagtcg
     1561 aggcggcggc ggcgctcgat gtcgtggacg cactcggggg catcccggtg ctgtgcgtcc
     1621 gggcatccga cggcgacgcc cgcgaacggc accagggtgt cagccaccac acgctcaccg
     1681 cgatcgagct gacgcggtcg gcgcccgtcg tggcggccgt gccccacgag gtgctcacgt
     1741 tggagggcgt cgtggtcgct gaggtcgatc cgcccgacgt ccgcgacatc ctcggcgccg
     1801 ccggcctgcg catcacgacg atgggtcgcg acatcgacgc ggacccgctg ttcttcaccg
     1861 ccgccggagc ggccgcggcg gccgcggtca ccttcctgag ctgactctgg agcgctcagg
     1921 tgacagttcc tgtcacctga gcgctccaga acgggccggg gcgggcaggg ttggcccggc
     1981 gggaacgtct gttcccctaa gatccctggc cggatgacga cgaagctcga acgcctcctc
     2041 aacctcactg ccttgctgct cgacaccgag cggcccgtgg gcatcgagca gatcggtcgg
     2101 gtgatcgagg gctacccgcc cgacccggcg agcttccggc gcacgttcga gcgcgacaag
     2161 gacgacctgc gcgagatggg catccccgtc gaggtcatca acaccgacgt ccacgacaag
     2221 acggcgctcg gctaccggat cctccccgag cggtactacc tcgccgaccc gggcctcgaa
     2281 cccgacgaga tggccgcgct caagctcgcc atgggcgcga tccggctcga cggcatcgcc
     2341 acccaggaga cgttgcgcaa gctcggcgac gccagcggcg acagcgccca acccgagctc
     2401 gcgtcggtgc cgaccccgcc cgtcatcggc gacctgcacg ccgccatcaa cgagcaccgg
     2461 ctcgtccagt tcgcctacaa cggcgagcag cgcaccgtcg agccgcaacg cctcgaattc
     2521 cacaagggcc gttggtacct ccggggcttc gaccgccttc gtgaggatcg gcgcagcttt
     2581 cgcatcgatc gggtcgaggg gtcgatcacg gccggtgaac ccgggtcgtt cgagccgtcg
     2641 ggccacgatg ccggaccggc cggcttccgg ttcgacccgt ggcgacacgg cgacgacgcc
     2701 ccggtgaccg cggtgctccg catcgacgcg acacaggcgg cgttcagccg cagcctcgtg
     2761 caggaggagg cggtgtggac cgacctcgac gacggctcgg tcaccctcga ggtcgaggtg
     2821 accaaccggc gggcgttccg cacgttcgtg ctcggtctgt tggagcacgc cgagatcgtg
     2881 agcccgcccg aactccgtga ggacctggtg gagtggctcg aagcgacgat cgcgtcgagg
     2941 ggcgccgtat gaagccccgc gtcaccgcat cggatcgctt ccggcgaatc gtcgcgatcg
     3001 tcccatggat cgccgagcgc ggcgggccgc ggatcgacga ggtgtgcgcc cagttcgaca
     3061 tcagccggga ggacctcctc gccgacctcg acgtgatctt catggtcggc atcccgccct
     3121 acacccccga cgagctgatc gacgtgatca ttgacgacga ccgggtgtgg gtcacgctcg
     3181 gccgttactt cactcgaccg ttgcgtctca ccgcccagga ggcgctcgcg gtgctcgctg
     3241 ccggctcggg tttgatggcg acgaccggcg ccgaccccga gggcccgctg gcccgggcac
     3301 tcacgaagct gcaacgcgtc gtcggcatcg gcgagcccgg atcgctcgag gtcagcctgg
     3361 gcggcgccga cagcggaacg ctggcgctgc tccagcaggc gacgaccgaa cgacgccaga
     3421 tcgagatcga ctactactcc tacagcaccg acgagagccg ggtgcgcacg atcgacccgt
     3481 accgggtcta cgcgaccgac ggcaactggt acgtgatcgg gtggtgccac tccgccgacg
     3541 ccgagcgcct gttccgtgtc gatcgcatcc ggagcgccca cctgaccgac ggcagcttcg
     3601 agctgccggc ggagctgccg gcggccacgt cgtactccgg gtcgggggcg gacccgcgga
     3661 tcgccatcga agtgggtccg gctgaccggt ggatcgccga gcactatccc accgaccggt
     3721 tcgaagagct ccccgacggc cgctaccgcg ccgtgctcgc cgtcggtggc gacgcgtggc
     3781 tggagcggct gctgcttcgc ctcgggcccg aggcgacggt gctcgacctt cccgacggcg
     3841 aggtcgacgg tcccacgatg tcgagcgacg cggctcgggc gctgaccgag ccgatggcgc
     3901 agtcgatcat cggtctctac cgctcctgat ccggcgggcc ccgcagcaaa atcgcgctcc
     3961 ggggcagatt tcagggatcg tcccgttagc ctgaccggtg tgccggtgag ctcctcgacc
     4021 gaaccgcttg acgatcgcct caccgacatg ggtcgggcac tcgcgaactc gttgttgggc
     4081 gacgtcacgg tcgcccgccc gatccgcgag cccgccgagc ccgaggtgcc cctgagtcgc
     4141 cgggcccgtc gcgagcagcg tgagcgtgcc gctgccggcc gcggtgaacg ccacgagttc
     4201 ccgcccccaa ccgcagcggc gccgtcggct ccggccgctc ccgcccgccc tgccgcaccg
     4261 tcggcccagg ccgctccggc cagctcggcc ccaccgtcgg ctccggtggc tcgggccggc
     4321 gagggagcag cactcgttcg gccgggtggc gttcccggcg ttcgtgacgc cgccgcgcca
     4381 cccgtcgcac ccgacgggac ccagttccag atcgctcggc cccgacgccg gtcggcgccc
     4441 gatcccgctg cgccctcgtc gtcggctccg acaccgccgc gtcccgcagc gcccgcagcg
     4501 cccccggtcg ccccgcagcg gcgacccgcc ccgcccacac aacgcccgga ctcgccgcgt
     4561 ccgcagcgac cccaggcgcc cgctgccccc gccgccgcat cggcaccggc aaccgctcca
     4621 gcccctgctc ccgcgcctcc cgccgcagcg cgggccggcc gcgtcgacgc tcgtccggac
     4681 gagcaaccac cgctgcgacc tcgcctccgc aacctggccc ccgacccgta cgccgcgctg
     4741 ccgtggccgg ccgacgaagc ggtctcctac cacgacgaca tccgccgggc ccccgagtcc
     4801 cggcccggtt cgcggatccg cgatctcgac ctgacggtcg cccgcgctga gccggcggag
     4861 cagcccgagg atccgaccga ggagtcaccc cggggcggcc tcgtccgcac ggtcgtcgag
     4921 tgggcgatgg tgctgggcac cgccgcggtg gtggccctcc tgctgcagtt cttcgtcgtc
     4981 cagctgtacg agatcccgtc ggagtcgatg tacccgaccc tgcagaacaa cgaccaggtc
     5041 ctcgtcaaca agctcgccta tgcggtcggc ggcggcgtgg aacgcggcga cgtcatcgtc
     5101 ttcaaacgcc cggagtcgga acgcaccgac gaccccgacc agccacccca gctcatcaag
     5161 cgcgtcatcg gcctgccggg ggacgtggtc gaggcccgtg gtggtgtggt gtacatcgac
     5221 gggaagccgt tggaggagac cggcacgggt cactacctcg acccgagcgt cgtcaccaac
     5281 aacctcccgc agcccgtcac cgtgccccga ggccacgtct tcgtgatggg cgacaaccgt
     5341 gagcgatccc atgacagtcg ttatttcggt gcgatccccg aatctgacat cattggccga
     5401 gccgtggtca tagcgtggcc cttcagccgt tggggatcct tgtaacgatg ttcgaccaga
     5461 ccaacacgac gggcgttccc cccgagtacc gcccgctgtt ggatccaccg gccccgccgg
     5521 tgaccgcgga accggtccca tcggaacggg tgctcgagct caacgccgaa ccggcggcca
     5581 atcccaaggg caccgccatg ggatgggcgc taacgatcgc cgtcgcggtg gtgctcacga
     5641 tcgtcgtcaa gacgttcatc ctccaggcgt actcgatccc gtcggagtcg atggtcccga
     5701 cactcaacgt cggcgaccgt gtcgtcgtgt tccggctcaa caccgacccc gcccggggcg
     5761 acatcgtggt gttcgatcgt ccggcgaacg atcccaagac gagcccgagc gaccccgacg
     5821 tgctcatcaa gcgcgtgatc gggttgcccg gcgacgtcgt cgagagccgt gacggcgagg
     5881 tctgggtcaa cggcaagcag ctcgtcgagg actacctccc cgacgggacg acgacgatca
     5941 tcgacacgcc gatcacggtg ccgagcggga agttgctggt gctcggcgac aatcgttccc
     6001 ggtcgttcga cgggcggatc ttcggcccca tcgacaagga cctggtcgtc gggcgcgcca
     6061 tcgcccgtat ctggccgctg tcgcgcctcg gcggtctcta gcccctcgcc ggttactcga
     6121 ggaactgcgg cgcgtccgga cgggcgatcg ccgcggcagc gtcgaccagg cgatcggggt
     6181 agctcgagat cacgccgtcc atgccgatgt gcagcagccc gaggatcatg cggtcatggt
     6241 gggcgtcggt gccgaagcag cgccggccga agcggtggaa caacgcggcg cgcccaccgt
     6301 tccaatcggc ctgtggcagg agcttggcgt cgatccggtc ttcacggagc ccagcggcgt
     6361 ggcgttcggc cccggcgccg aggtcgttga gcgtggtcgt gtcgacgagc cgaacgagct
     6421 cggaccgggc ccgccacgcc aggagtgcgg cccggtcgga ggccgccaac cacagccgat
     6481 cgagggcccg gtggcgggtc gcgagcgcaa tggcctcctc gacggcgtcg accgtccgga
     6541 cgtggacgag gaggtcgagg gtggcgccgt gctcctcgta gaagccggcg agggtcacga
     6601 cgtcgtcggg caggtcggcg ctcgacgtgt cggcgatgcg tcggcggcgc agacctccga
     6661 ggcgggcgtc gcgccgcagg accggcacgc cgtcggcagt gacctgcagg tcggtctcga
     6721 tgcccgtggc tccgagccgg acggccagcc ggaacgactc cggggtgttc tcgtcggtgt
     6781 ggccacgggc cccgccactc gcgaacagga tcgctggtcc ggcgagtggc tcctggcgag
     6841 ggatcatccg ccgattgttg ccggtgggtc cggttggtgc gaacgccggg tcgtcgcaac
     6901 ggcgcagatc aggagaacac cacaagttcc gagggcgaaa tcttcaactc acccctggtg
     6961 ggtgccgaaa gaagtggaag ctcatcctga tagcgaagga acgactctca tggctttgat
     7021 ccgggcgact tgcagcgaat gtggtgacgt cgaattgcgt tcgcgcgacc tgcaggtccg
     7081 catctgtggc gacaccggtg acggtaccta cctgttccgt tgccccgtgt gccgcatgac
     7141 ggaggtgaag aacgccgatg accaggtcgt cgacatcctc gccgccgccg gggtgaagtg
     7201 cgtcgagtgg cgtctgcccg ccgagatgac cgaacggccg accggcgagc cgatcaccca
     7261 cgacgacgtc ctcgacttcc acgagatcct gggcggcgac gactggttca ccacgctgtc
     7321 ggagatgacc cgacacggct gacgcccgtc gcagtcgggc ttcccgggcg gttcccgtcc
     7381 gcaggtaggc tgaccgcatg ctcatcggag gactgctcgg catcctcgac agcctcaccg
     7441 gcggcgaagt catcatgatc ctggtcgcgg cgctgctcgt gctcggcccc gagcgcctgc
     7501 ccgaaacggc tcgcacgatg gggcagtggc tggccaagct caagtcgatg accggcaacc
     7561 tccagagtga ggtccgcgag gtgctcgacg acccgtcgat gaagccgatc cgcgatctcg
     7621 gcgagttcgc cgcggcgccc cgcaagaaga tcgtcgagtt cgcgacggcg gcggaggccg
     7681 aagccaacct cgccaagcag gtcgcagcgg aggccgaggc cgccgccgcc gaggcggaag
     7741 ccgctgcggc gaaggcccgc gccgccgccg acgaagcggc tgccgaggcc gaggaggccg
     7801 agcaggtcgc cgaggacgcc gccgaactcg tcgaggagac accgcccgac gagcccgcgc
     7861 cgggcgagcc gggtgcgggc cgtgaggcca gcatctgggc cgacctcgag gacgagccgc
     7921 tcgaggacga gccggtcgtc gccgagccgc tcgaggacgc gccgctcgag gacgggacac
     7981 cctggtcgtc gaaccatggg gcgatccagg cgtcggtgga ccgaggagcg tcggagccaa
     8041 cgtgacgcgc atcgccaact tcagagccat gcgccaacag cgtcgtcggc cgtcgtcgcc
     8101 tgacggggcg atgagcctca tggatcacct gcgcgagctg cgcaaccgga tcttcaagtc
     8161 cgccctcgcg gtgttcgtcg ccggaatcgg cgtgtacttc gtgtaccacc gcctgtacga
     8221 cgtcctcgtc gagccgtact gcgaggccat caagaacacc gcccaggagt cgtgcaagct
     8281 cctctacacc gacccggtgt cgccgttcgc gatgcagctc cgggtgtcgg cctatgtcgg
     8341 catcctcatc gccctgccgg tcatcttctg gcagctgtgg cggttcgttg cccctgggct
     8401 ctaccagaag gagaaacggt acgcgatcgc gttcgtcgcg agttccgtgg tgctcttcct
     8461 gctgggcgca gcgttggcct ggtattcgct cccgaagatc ttcgagtggt tggtcgacca
     8521 ggccggcgac gccctggtcc agaccaaggt cgacgagtac atgagcctgc tcgcgctcat
     8581 ggtgttcgcg ttcgggttga gcttcgagtt cccgctgata ctgctggcgt tgcagctgat
     8641 gggcgttgtg tcgcccgaca ccctcgccgg cgtccgccgc cacgccatcg tcggcatcgt
     8701 cgccgtcgtc gccatcgtca ccccgggcgg agatcccgtc agcctgttcg cgctgtccat
     8761 cccgttgtgc atcttctacg aggcgtccat ctggatcgcc cgcctcatgc tcggccgccg
     8821 cgccaagaac cgcgaggcga cggacacggc cgcgtgaccg tgtcaccgtc ggactccgct
     8881 cccttcacgc tcgaccgttt ccaactcgag gcgatcgccg agttcgacgc cggccgctcc
     8941 gtgctcgtcg ccgcacccac gggttcgggc aagacggtcg ttgccgacgc tgccatcgac
     9001 atcgccctca acgccggcgg caagaccttc tacaccacgc ccatcaaggc gctctcgaac
     9061 cagaagtaca gcgatctcgt cgcccgcctg ggcaccgagc gggtgggact gctcaccggc
     9121 gacaccgcga tcaacggcga cgcgcccgtg gtggtgatga ccaccgaggt gctccgcaac
     9181 atgctctatt cggggtcggc cgccctcgac aacctgcacg tggtggtgct cgacgaggtg
     9241 cactacctgc aggacgccta ccggggcccg gtgtgggagg aggtcatcat cggcctcccg
     9301 cccaacgttc ggctcgtctg cctgtcggcg acggtgtcga acgccggtga gttgggggag
     9361 tggatcgaga cggtacgcgg tccgacgtcg acggtcatcg agcacgagcg tcctgtcgag
     9421 ctcgagaacc tgtacctcgt cggcgaccgc tcatcggatc gtgaccacct catcccggtt
     9481 ctggtcgacg gtcgccccaa ccccgagggc taccgattcg acggcgatgc cagcggcgga
     9541 cgggggcgac acacgggggc gccacgggat cgccgtcgcc ggttcgtgac gccgcgtcga
     9601 ctcgagacga tcgagcggct cgcggaggaa gacctgctgc cggtgatcta cttcatcttc
     9661 agccgcaacg cctgcgacga cgcgctcgcg atcgcccgcg acagcggtct tcggttcacc
     9721 gacgccgagg agcgcatccg gatccgcgag atcctcgagg gctcggtctc gatgctgagc
     9781 gactccgatc tggacgtcct cggctacgac atgtgggccg ccgcgctcga gcagggcatt
     9841 gcgtcgcacc atgccggcct gatcccggcg ttcaaggaag cggtcgagcg ctgcttcgtc
     9901 gagggcctcg tcaaggtggt gttcgccacc gagacgttgg cgctcggcat caacatgccg
     9961 gcccgcagcg tggtcatcga gaagctgacc aagtacaacg gcgacaccca cgagttcctc
    10021 accccggcgc agttcaccca actcaccggc cgggccggcc gccgcggcat cgacgacgag
    10081 gggttctccg tcgtgttgtg gtcgccgttc gtgtcgttcg cccaggtcgc cggcctcgcc
    10141 ggcagccgcg agttcccgct cacgtcgtcg ttccggccca cctacaacat ggccgccaac
    10201 ctggtccgcc ggtacgagaa gaccgaggcc atgtccctcc tcggacagtc gtttgcccag
    10261 ttccaggccg atcgggccgt ggtcacgctc gagcggcggc tggcgcgcga ccgcgagcga
    10321 ctcgcggtga tcgacgagca ggtcgggtgc gagcgcggcg atgtcgccga gtacgccgcc
    10381 ctcgacgagc gggtgaattc ccagcgcacg aatcgtcccg acggtcgcat cgcgatcgaa
    10441 cgcagcgtca gcctgctccg acccggcgac gtcattgctc cgcggctgcg tgaggacacc
    10501 accgcccggc tccacctcca cccgcacaag cccgtgcgcc tcgcggtcat ctcggtcgcg
    10561 taccggggca agggttcgtt gcgtgtccgc accgtcgacg agcggggcga cgccgtccag
    10621 ctggtggtcg ccgacttccg ggagccctcc cgtgccgtcg acgagatcga gctgcccacc
    10681 ccctacgccc cccacgatcc ggcgttcctc gacgagacgg cgtcgcgcct ccgggccgcc
    10741 cgggcgacgg tctcgaccgc gccgtccggg gcgtcgcggt gggatcgcct cgtggccgaa
    10801 ctcgagtccc atccggtcca cgactgccct gaccgcgacg accatctggc cgcctaccgc
    10861 gagcgctcag ggctggagcg tgacgtcgcc cggttgtcca agcagctgca ccagcggtcg
    10921 tcgtcggtcg tccgccggtt cgaggcggtg gccgatctcc tcggccatct cgggttcctc
    10981 gacgggtggg ccctcaccgc accgggggag cgactggcgc ggatctacca cgaacgtgac
    11041 ctgctggtgg cgctggcgct cgacgacggc gtgttcgacg ggctcgacgc ccccggcgtc
    11101 gcggcgctgg tcgcggcggt cgtgttcgag gagcggcgtc ccgacgggtc ggagccggtc
    11161 taccccaacc ggaacctgcg ccagcggttc ggtcggctcg aacggctcgc cgacaagatc
    11221 gcccgcgccg agcgcgagcg ggggttgccg cagacccgac cgcccgatcc cgggttcatg
    11281 gcggtgtgcc acgggtgggc gtcggggcgg gatctcgggg gcctgatcga gggggagatg
    11341 tcgggcggcg acttcgtgcg caccacgaag atgatcatcg acgtgctggg ccagatcgcg
    11401 gagatcgccc ccgacctcgg cacgcggtcg cgggcggcgg cggccgtcga ggcgatccgg
    11461 cgcggggtgg tggccgactt cgggccctcg gaagccctgc cgaaggcagc cgaggaactg
    11521 ccgaaggcag cggaggatcc gccgaaggcg gggacgtgat ccgcaagggc gaggtctggg
    11581 gccggcccgt cgatctcgac ggcaccgagc cggaggcgag ttccgacgcc gaggtcgcgc
    11641 gcctgatgag ccggccactc gactcgccgg tcgtcctgcg tggcggcgac ctgtaccact
    11701 cgctcggtgg tcgggcgagc tcgccggtcg acctcgacgg accggtgatg gccctgccga
    11761 tcgatctcgt cgccgtccgg gtcgacggcg tcgaccgcgt ggccgcggcc catgtggtcg
    11821 ctcgccggcg ctggtggtcc ggggagttcg ccgtggcgat gaacggcacc cacctcggcg
    11881 agtggaacct gggcccgaag gcgcacccca acgacggact gatcgacgtg accaccggcc
    11941 ggctcgacgt ccgcgaccgg tgggaggccc gccgccgggc ccacacggga acgcacctgc
    12001 cgcacccggc gttgtcgacg tcccgggtca cgagcacggt gtggacgttc gaccgaccca
    12061 cgcccgtcta tgtcgacggt gtggccgtgg gttcggcccg ccgggtcgaa ctcgaggtgc
    12121 agcccgatcg gggcagcgtc gtcgtcggtt gaggcgtcag gggtcgccgc cgtggcgcgt
    12181 ccgctcgccc gctgacggca cggttctggc tggaccccgt taccatccca gccatgcaga
    12241 gttggtttct ctcggaatcg cccggcgagt accagtgggg ggaggtcccg acacccgagc
    12301 ccggcgtcgg cgagatccgc gtccggctgg tcgcgagcgg tgtgaaccac atcgaccact
    12361 ggcagacgcg tggcctcccg aagcccaagt cgttcccgca cgtgccgggg tccgacggtg
    12421 cgggggtcgt cgacgcgatc ggtgacggcg tcactcgctt ctcggtcggc gacgaggtcg
    12481 ccatcaacgc ggcgcagacc aacgccgcag cgatcgagaa gttcgggatc gacagcgtgt
    12541 tcgacccgag cctccagttg atgggggagc accggtgggg gtgccacggt gaacaggtcg
    12601 tcatccccga ctacggcgcc gagctgcgcc cggccggtcg ctcgtgggag gagtgcgcgg
    12661 cgtaccccgt cgcgctgacg acggcgtggc ggatgttccg cagggcccgg ctgcaggccg
    12721 gtgagatcgt gctcgtcacc ggcatcggcg gtggcgtcgc caccgcggcc cagatgctcg
    12781 cccagcacat gggagcccgg gtgttcacca cgtcgcgtga tgccgccaag cgggcccggt
    12841 cgatcgagct gggcgccgag gactcgttcg attcccacga gcggtatccg atcaaggccg
    12901 acgtgatcgt cgactcgatc ggtccggcga cgtggaacca gaccgtggcg gcgctcaaag
    12961 ccggtggccg cctggcgacg tgtggcggga cgtcggggtc ggacgtgcag ctcagcctgc
    13021 cgcggctctt cttcaagcag cacgagatcg tcggttccac tctcggttcc caggaggagt
    13081 tcgcgtacgt cacgcagctc atggccgagg ggctccccgt ggtcatcgac gaagtgttcg
    13141 cgtgggacga ctatccgcgt gccgtcgagc gcatccgcaa ggccgaccag ctcggcaagc
    13201 tgatcctgcg ccacccctag gggtttcgta ctgcgaggag ggccgctgac ggtcctcctc
    13261 gcagaacaaa gccctgcggg tcgggcgtgt ccggtcgggg tctaggcttt cgccatggac
    13321 gtcgagggtg ccaagcggtc cgtcaccgaa cgcatcgagc agcgtcgtgc cgacctgatc
    13381 gacacgtcgc accagatctg ggcgaacccc gagctgtgct tcgaggagca cttcgcccac
    13441 gacc
//
LOCUS       DAOSOA010000053        13221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4719_length_13221_cov_5.934376, whole genome shotgun sequence.
ACCESSION   DAOSOA010000053 DAOSOA010000000
VERSION     DAOSOA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13221
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4719_length_13221_cov_5.934376"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PKY13_05320"
     CDS             complement(<1..783)
                     /locus_tag="PKY13_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT79383.1"
                     /translation="MTDHLRLPLAGSPMTGDEFVARCSAMAPLLAEHAAEGEELRRVP
                     AATIEAVAAADLFRAVVPTSMGGHGLGLAALSQGTRELAHGCAASAWTLSFLMLHGWL
                     LAKFPTDGRDEVFAAGPTPMAPAPLAPTGTATAVSGGLIVSGRWEWATGIAHADWVMV
                     NAVQTEPEFELWFAALPRDEVEVDDVWFTSGMRATGSNTVIVRDRFVPEHRLLRARSL
                     LDSTDAIDGDGMAGLPVPAVLGLSASAVALGALVAAGVIGRAG"
     gene            complement(780..1325)
                     /locus_tag="PKY13_05325"
     CDS             complement(780..1325)
                     /locus_tag="PKY13_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT79384.1"
                     /translation="MRPVLRESVPSQRPRDQWLSAGQELLRRGGVKAVKLGALTAELG
                     LTTGSFYHHFDGMAAFLDELARFYGADQVVAILAAAEADDPVERLRNLVRVSRHDARA
                     ALDVAMRDWAGSNDLAASAVRDADEQLLRFVERAFRDLGHDRAAARVRALTLVSVGVA
                     RVIPPWRLPRTTAEQILEILT"
     gene            1407..2471
                     /locus_tag="PKY13_05330"
     CDS             1407..2471
                     /locus_tag="PKY13_05330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOT79385.1"
                     /translation="MTTTLPIAETADMITPAWLGAALGRPVTAVATSPLGTGQMCDSV
                     RVTYRCGDDSDDLGSTVIAKLPASDPVSRNTALVMRSYEKEVRFYEVLAGELPVRTPG
                     LRYGDIDVESGSFVLILDDLAPANQGDQLAGCSPDVAALAVAELVGLHAPRWDDPTLL
                     DLPWLRNDDPDGRAMMAMLMPMLWDGFRDRYGDAVEDHVRMAGDAFFPRIAEFMELPD
                     DGRTIIHGDYRLDNLLFDDAGPTVAVVDWQTCAIGPAMHDVAYFVGAGLDEHDRREHE
                     GRLVDDYHGALVGAGVAGYDRERCWRDYRRGSWAGLLMAVGAAMMVERTERGDAMFLT
                     MASRHARHALDLDATDAVAP"
     gene            complement(2494..3480)
                     /locus_tag="PKY13_05335"
     CDS             complement(2494..3480)
                     /locus_tag="PKY13_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT79386.1"
                     /translation="MPLLVLRFDLRNPAFAGVTLTDRYAAALEMCEWADANGVLAVVL
                     SEHHGSDDGYLPSALTMAAAVAARTSHVAIWVSAVAAPFHDPVRLAEEAVVVDSISAG
                     RLTLVLANGYVPSEFDMFGVPIGERVARTTEAVLAVRAARDGAPVQVGERSVKVTPAP
                     DRRNGRTGPTVLLGGSGPAAARRAARIADGFQPSNPEAWEVYRSARIDAGHADPGGYL
                     AEPTGFTHVSLDPDAAWERIAPHALHESQSYGRWAIEAGIEGASGYRDSDDAAELQAS
                     GRYRVVTPDDLVAELSADPGALLVSSPMMGGIAPDLAWESLRLIEHEVLPRC"
     gene            complement(3500..4099)
                     /locus_tag="PKY13_05340"
     CDS             complement(3500..4099)
                     /locus_tag="PKY13_05340"
                     /inference="COORDINATES: protein motif:HMM:NF036523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT79387.1"
                     /translation="MARSEVTTRPAVAGNASQAARRQRIVDAALELLQDRPYDQIQMR
                     DVAEGAGVALGTLYRYFPSKEQLFAHVLVEWSRRFEPGRRRGPAPETDADRLVAVLRA
                     AARAFERHPNFFQLIALLEVISDPDVAEPFHEYSGAFSTALSDALHDVDPSDVVVIST
                     MSSALIGTLLRSWWLGRISMRSVYDQLDRSVALMFHGVG"
     gene            4209..4712
                     /locus_tag="PKY13_05345"
     CDS             4209..4712
                     /locus_tag="PKY13_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HOT79388.1"
                     /translation="MGINQRNQITMSDDEVAAFLDQSRSITMATIGADGTPHLVAMWF
                     GIIDGKLYFETKLKSQKAVNLRRDPRIVCAAEAGHSYDELRGVSIEGTTTLIEDQDDP
                     EYWAAGVNIWERYQGPYTDEMKPFVEIMMNKRVVVRVEPTRVRSWDHRKLGMDHMEPG
                     GTTAAYL"
     gene            complement(4796..5659)
                     /locus_tag="PKY13_05350"
     CDS             complement(4796..5659)
                     /locus_tag="PKY13_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT79389.1"
                     /translation="MAIPDGIGTIDTMIGFPHGDMKAVYEFITRQTRDRESKEEFEFP
                     AEYMFKQVPEKEFVDADDPVAVTVDAMDRVGIAQGMIGVDEISQEAMRRFPGRFFASA
                     ECDPHAGVEGLRRLRRLKDEFDIKAVGCFPAGTFPQVPINDKLMYPIYATCCELDIPI
                     FCCAGVPGPRLKMNPQRVELIDEVMYDFPELVFVTRHGCEPWADLAVKLMIKWPNLYY
                     STSAFAPKYYPKAIIDYANTRGAHKVIYAGYFPMGLSLDRIHSEMPNVPFKDEVWPGF
                     LRDNAARILRL"
     gene            5737..6699
                     /locus_tag="PKY13_05355"
     CDS             5737..6699
                     /locus_tag="PKY13_05355"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT79390.1"
                     /translation="MSPADLPVPIDPEFAEMLAMLPEIRITAETLPEFRDLLAALQTT
                     PPVDGVTAEEIDVDDHVSVRVLRDGRTEPGGADDVDGAARPAVLWIHGGGYVLGHHAM
                     DDGHLGPWVLELGCIVVSVDYRLAPEHPYPTPLEDCYAALWWLHQHAGDLGVDPDRIG
                     VAGLSAGGGLAAALAVLARDRGELPIAFQLLDSPMLDDRRITPSSNYDGLPVWNRHSN
                     EFGWKAMLGDLAGRDDVPATASPSRATDLSGLPPAFVVVGGADGFRDEDIDYAVRLQQ
                     AGVPTELHVFPGAPHGFGLFVGSSIAQQAEAAQRNWLARQWGSS"
     gene            6696..7457
                     /locus_tag="PKY13_05360"
     CDS             6696..7457
                     /locus_tag="PKY13_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT79391.1"
                     /translation="MTRTYLITGSASGIGRATFELLTGQGHRVIGVDLHDAEVVADLA
                     TAAGRAAMVERVNELTDGVLDAVVANAGVQDTPEGCVRVNYFGAVATLEGLRPLLARG
                     TAPRAVATCSSSVLNENSPRLVAALVAGDEEDAVAASVNTGLAAYPSSKRALARWIRR
                     TAPTSGWAGVGIGLNAVAPGVVETAMTAPLLVDPAMVELIEAAVPMPHGGIAQPGHVA
                     DLIAYLTSPNSARICGQVVFVDGGADVVLRGEDIW"
     gene            7454..8647
                     /locus_tag="PKY13_05365"
     CDS             7454..8647
                     /locus_tag="PKY13_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT79392.1"
                     /translation="MNFDDSPEEHAFRLKVRAFLDEHATLRTGTEADWWSGPTDESDE
                     SDLAFLRRCQDWARTVYDHGWAGIAWPTAFGGRGESVAQMIVWADEVSRYDVTIGFVG
                     IGHGLVGPPLMRHATPEQQQRYLAPLLRGDEIWCQLFSEPGAGSDLAALSTRAELDGD
                     DVVVSGQKVWTSEAHHADFGILLVRTDPTAPKHHGITFLIVDMRSPGIEVRPLRQASG
                     QAHFNEVFLDEVRIPVANVVGEIDGGWKVARTVMASEAGMIGTGGNDPANYDSMLRLA
                     RGLGRTADPLVRQGLADLHAREQMIRFMGMRFQTFLTTGRGTPPDPSVLKNFHTRKNA
                     RKADLGLAIQGAPGLLDGADAWEHGLWQRSMVMQFASRIGGGTDEVHRNMIAERALGL
                     PRDPR"
     gene            8713..9471
                     /locus_tag="PKY13_05370"
     CDS             8713..9471
                     /locus_tag="PKY13_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOT79393.1"
                     /translation="MTEPTPPTPTVRLDDLIDAVTAVHPDELQQLSGAVLAADRLGEL
                     ADHLVGHFVDRARRSGASWSEIGRSMGVSKQAAQQRFTPRSGADRGVDPSQGFERLTT
                     RARNVIVAAHDVAVARSNVEVTPAHLVLGLLSEPDALAGVALQAQGIPFEAVRAAAMA
                     ALPGPVSEPPALVPYDAAAKKVLQLTFREALRHGHSHVGTEHLLLALLEHEDGSGPLN
                     RAGVDKAATEVSIADALASRPARPPASPSEAPLA"
     gene            9483..9794
                     /locus_tag="PKY13_05375"
     CDS             9483..9794
                     /locus_tag="PKY13_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOT79394.1"
                     /translation="MPIPAFRLLALTEAVSWLLLIGAAVLKRAADLPEATQIMGPIHG
                     VIFLVYAGAVLYVRDDLKWPAGRTLMALFAALLPLGTYLIVERRYLAEARVADLDAAG
                     A"
     gene            complement(9860..11395)
                     /locus_tag="PKY13_05380"
     CDS             complement(9860..11395)
                     /locus_tag="PKY13_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOT79395.1"
                     /translation="MTGAGLLVCGTASDVGKTIVVAGLCRALARQGVRVAPFKAQNMS
                     LNSFVTADGLEIGRAQAAQAWAAGVEPEAAMNPVLLKPGSDTSSQVIVMGRAVGERDA
                     STFWGDDGPVAQSSLLDVVVDAYRDLAARFDAVICEGAGSPAEINLRRSDIVNLGFAR
                     ATDTPVLLVGDIDRGGVFASFVGTLAVLDAADQALIAGFVVNKFRGDVSLLEPGLHML
                     ADLTGRPTLGVLPFVRGLGVDAEDAIDFELLRSGPAPLGDDVLRVNVVAFPRMSNHTD
                     IDALALEPGVVIRFVDQPAQLVDADLVVLPGTRATVSDLAWMRERGIDIAVARHAAAG
                     RAVLGICGGYQMLGSSIEDGVESGAGTVAGLGLLPVDTSFGLEKVLARPSMVLADGSV
                     IEAYEIHHGIVGSSGGEPFVAAADGSGEGCRVGAVAGTVWHGVLENDAFRRRYLAEVA
                     HACGRRFVVASDISFAAERAARLDRLGDLVADHLDLVAVEELLAGPRPDLPHLRLSRS
                     SSG"
     gene            complement(11400..12512)
                     /locus_tag="PKY13_05385"
     CDS             complement(11400..12512)
                     /locus_tag="PKY13_05385"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HOT79396.1"
                     /translation="METQAAIMWEPGGKWSVETITLDPPKAGEVLVEMVASGMCHSDD
                     HARTGDLPAVTPLIGGHEGAGIVMEVGDGVTKVKPGDHVVFSFVPACGICQSCADGHS
                     NLCDNGAALLVGAQLDGTSRHHAADGTDLWTMVCLGTFGKHTVVNEMSCVKIEDDIPL
                     ELACLVGCGVTTGWGSSVYAAEVRAGDNVAVIGVGGIGAAAVQGARLAGARNIIAIDP
                     VEFKREQAMKFGATHTANSIGEAFSLIQEITWGRMCDKIICCVGVGDGEQIGPIMQLC
                     AKRGRVVVTNIHPASEGSINISLCDLTLMEKQLVGTIFGSANPNSDIPRLMHLYRSGQ
                     LDLAGMVTREYDLEDINEGFADMLAGKNIRGVLRYK"
     gene            12788..13204
                     /gene="acpS"
                     /locus_tag="PKY13_05390"
     CDS             12788..13204
                     /gene="acpS"
                     /locus_tag="PKY13_05390"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOT79397.1"
                     /translation="MWETTAQGTVRVGTDLMSVSEVASSIDRFGDRYLRRVFTPAELA
                     ACAGPSGPSAARLAARFAAKECAVKILHPIDGVNFHDIEVQTDRDGRPSIAFHGTMAD
                     RARALGIVDASLSLSEERGLATAILVAVIAPAPRLR"
BASE COUNT         2042 a         4701 c         4592 g         1886 t
ORIGIN      
        1 gccagcgcgg ccgatcacgc cggcggccac cagcgcgccc agcgccacgg ccgacgcgct
       61 caacccgagc acggcgggga cgggcagtcc ggccatgccg tcgccgtcga tcgcatcggt
      121 gctgtcgagc agcgaccgcg cccggagcag ccggtgttca ggcacgaacc gatcgcgcac
      181 gatcacggtg ttgctgccgg tggcccgcat ccccgacgtg aaccacacgt cgtccacctc
      241 gacctcgtcg cggggcagcg ccgcgaacca cagctcgaac tcgggctcgg tctggaccgc
      301 gttcaccatg acccagtcgg cgtgggcgat gcccgtcgcc cactcccacc ggcccgacac
      361 gatcagccca ccggacaccg ccgttgcggt gccggtcggg gccaggggtg cgggcgccat
      421 cggggtcgga ccggcggcga acacctcgtc gcgaccgtcc gtggggaact tcgccaacag
      481 ccaaccgtgg agcatgagga acgacagcgt ccacgccgac gccgcgcacc cgtgcgccaa
      541 ctcgcgggtg ccctgcgaca gcgctgcaag gccgaggccg tggccgccca tcgatgtcgg
      601 aacgacggcg cggaacaggt cggcagcggc cacggcctcg atcgtggcgg ccggcacccg
      661 ccgcagctcc tcgccctccg ccgcgtgttc ggcgaggagc ggggccatcg ccgaacacct
      721 cgcgacgaac tcgtcgcccg tcatcgggga cccggcgagg ggcaggcgga ggtggtcggt
      781 cacgtcagga tctccaggat ctgctcggcg gtcgtgcgcg gcaaccgcca gggtgggatg
      841 acccgagcga cgcccaccga cacgagggtc aacgctcgga cacgagccgc ggcgcggtcg
      901 tgcccgaggt cgcggaacgc ccgctcgacg aaccgcagca gttgctcgtc tgcgtcgcgc
      961 acggccgacg cagcgaggtc gttgctcccc gcccaatcgc gcatcgcgac gtcgagcgcc
     1021 gccctggcgt cgtggcggga cacgcggacg aggttgcgca ggcgttcgac cgggtcgtcc
     1081 gcctctgctg cggcgaggat cgccaccacc tggtcggcgc cgtagaagcg ggcgagctcg
     1141 tcgaggaacg ccgccatgcc gtcgaagtgg tggtagaagc tgccggtcgt cagcccgagc
     1201 tcggcagtga gcgcaccgag cttcacggcc ttgacgccgc ctcgacgcaa cagttcctgg
     1261 cccgccgaca accactggtc gcgaggtcgt tgggacggta ccgactcacg caggacgggg
     1321 cgcacgccgg cgaccctagc agccgtactt tttgttacgt ccacggcccc ggggtacggt
     1381 tgcaccagtc gtaccagggg ggtctgatga cgacgacact gccgatcgca gagaccgcgg
     1441 acatgatcac accggcgtgg ttgggagcgg cgctcggccg tccggtcacc gccgtggcga
     1501 cgagcccgct cggcaccggc cagatgtgcg acagcgtccg agtgacctat cgctgcggcg
     1561 acgacagcga cgacctcggc tcgacggtga tcgccaagct gccggcgagc gaccccgtca
     1621 gccgcaacac cgcgctcgtc atgcggagct acgagaagga ggtccggttc tacgaagtgc
     1681 tggccggcga gcttcccgtt cgcacgccgg ggctccgcta cggcgacatc gacgtcgaga
     1741 gcgggtcgtt cgtgctcatc ctcgacgatc tcgcgcccgc caaccagggc gatcagctcg
     1801 ccggctgcag ccccgacgtc gcagcgctcg ccgtcgccga gctcgttggc ctccacgcgc
     1861 cccggtggga cgacccgacc ctgctcgacc tgccgtggct ccgcaacgac gaccccgacg
     1921 ggcgggcgat gatggcgatg ctcatgccga tgctctggga cgggttccgg gatcgctacg
     1981 gcgacgccgt cgaggatcac gtccgaatgg ccggcgacgc gttcttcccc cggatcgccg
     2041 agttcatgga actgcccgac gacggccgga cgatcattca cggcgactac cggctcgaca
     2101 acctcctgtt cgacgacgcc gggccgacgg tcgccgtcgt cgattggcag acctgcgcga
     2161 tcggcccggc gatgcacgac gtcgcctact tcgtcggagc cgggctcgac gagcacgacc
     2221 ggcgagagca cgagggccgt cttgtcgacg actaccacgg cgccctcgtc ggtgccggcg
     2281 tcgcgggcta cgaccgggag cggtgctggc gcgattaccg ccggggctcg tgggccgggc
     2341 tcttgatggc ggtgggcgcg gcgatgatgg tcgaacggac cgagcggggc gacgcgatgt
     2401 tcctcaccat ggcgtcacgg cacgcccgcc acgccctcga cctcgacgcc accgacgccg
     2461 tcgcaccctg acccttgcac cctgacagat cggtcaacag cggggcagca cctcgtgctc
     2521 gatcaggcgc aggctctccc acgccaggtc cggtgcgatg ccgcccatca tcgggctcga
     2581 gaccagcagg gcgccgggat cggcgctcaa ctcggcgacg aggtcgtcgg gggtgaccac
     2641 tcggtaccgg cccgacgcct gcagctcggc ggcgtcgtcg ctgtcgcggt agccgctcgc
     2701 cccttcgatg ccggcctcga tcgcccatcg cccgtacgac tgcgactcgt gcaacgcgtg
     2761 gggtgcgatg cgttcccatg ccgcgtcggg gtcgagcgac acgtgggtga agcccgtcgg
     2821 ctcggcgagg taccccccgg ggtcggcgtg cccggcgtcg atgcgtgcgc tgcggtacac
     2881 ctcccaggcc tcggggttcg acggctggaa gccgtcggcg atgcgggcgg cgcgtcgagc
     2941 ggcagcgggg cccgaaccgc ccaacagcac ggtggggccg gttcggccgt tgcgacggtc
     3001 gggcgccggc gtcaccttca ccgaacgctc gccgacctgc accggcgcgc catcgcgggc
     3061 ggcacgcacg gccagcaccg cttcggtggt gcgggccacc cgctcgccga tcgggacgcc
     3121 gaacatgtcg aactccgagg gcacgtaccc attggccagc acgagagtga gccgaccggc
     3181 gctgatcgag tcgacgacga cggcctcctc ggcgagtcgg acggggtcgt ggaacggcgc
     3241 ggcgaccgcc gacacccaga tcgccacgtg cgaggtgcgg gccgcgacag cggcggccat
     3301 cgtgagggcg ctcggcaggt agccgtcgtc gctgccgtgg tgctcggaca gcacgacggc
     3361 gagcaccccg ttggcgtcgg cccactcaca catctcgagg gcggcggcgt accggtcggt
     3421 gagggtgacc ccggcgaagg cgggattgcg gaggtcgaag cggaggacga gcagcggcat
     3481 gaccggcgag tctgcccgct cagccgacgc cgtggaacat caaggcgacc gagcggtcga
     3541 gttggtcgta caccgaacgc atcgagatcc ggccgagcca ccagctccgc agcagcgtgc
     3601 cgatcagggc ggacgacatg gtcgagatga cgaccacgtc gctcgggtcg acgtcgtgga
     3661 gcgcgtcgga caacgccgtc gagaacgcgc cgctgtactc gtggaacggc tcggcgacgt
     3721 cggggtcgct gatgacctcg agcagcgcga tcagttggaa gaagttgggg tggcgttcga
     3781 acgcccgcgc cgccgcccgg aggacggcga cgagccggtc ggcgtcggtc tcgggggccg
     3841 ggccgcgacg tcgaccgggt tcgaaacggc ggctccactc gacgagcacg tgggcgaaca
     3901 gctgctcctt ggacgggaag tagcggtaga gcgtgccgag ggcgacaccg gccccttcgg
     3961 cgacgtcgcg catctggatc tggtcgtacg gccggtcctg caacagctcg agcgcagcgt
     4021 cgacgatgcg ctgtcgacga gcggcctggg acgcgttgcc ggcgacggcc ggccgggtgg
     4081 tcacctcgct ccgggccatg gggtgagcgt accaagcggc ccgttctgtc ccgcgaggcg
     4141 atgccgcctg gctcctttcg cgggacagaa ccggccgggc cggggcgtgg atctggcagg
     4201 atcacctcat gggcatcaac cagcgcaacc agatcaccat gtccgacgac gaggtcgcgg
     4261 cgttcctcga ccagagccgc tcgatcacca tggccaccat cggcgccgac ggcacaccgc
     4321 acctcgtggc gatgtggttc ggcatcatcg acggcaagct ctacttcgag acgaagctca
     4381 agtcgcagaa ggcggtcaac ctgcgccgcg acccccgcat cgtctgcgca gccgaggcgg
     4441 gccacagcta cgacgaactg cgcggggtgt ccatcgaggg cacgaccacc ctcatcgagg
     4501 accaggacga ccccgagtac tgggccgccg gcgtcaacat ctgggagcgc taccagggcc
     4561 cctacaccga cgagatgaag ccgttcgtcg agatcatgat gaacaagcgc gtcgtggtgc
     4621 gcgtcgagcc cacccgcgtg cgctcgtggg accaccgcaa actcggcatg gaccacatgg
     4681 agcccggcgg cacgacggcg gcctatctgt agggacgcag gtcagccgag gtaccgcagc
     4741 gtaagaagct cccgagggac gagggagcga acctgagcga aggtggccga ggccatcaga
     4801 gacgcaggat gcgggcggcg ttgtcgcgga ggaagccggg ccacacctcg tccttgaacg
     4861 ggacgttcgg catctcggag tggatgcgat cgagcgacag gcccatgggg aagtagccgg
     4921 cgtagatcac cttgtgggcg ccgcgggtgt tggcgtagtc gatgatcgcc ttcgggtagt
     4981 acttcggggc gaacgcgctc gtggagtagt agaggttcgg ccacttgatc atgagcttca
     5041 cggcgaggtc ggcccacggc tcgcacccgt ggcgggtcac gaagaccagc tcggggaagt
     5101 cgtacatgac ctcgtcgatc agctcgacgc gttgtgggtt catcttgagg cggggcccgg
     5161 gcaccccggc gcagcagaag atcgggatgt cgagctcaca gcacgtggcg tagatcgggt
     5221 acatcagctt gtcgttgatg ggaacctgcg ggaacgtgcc cgccgggaag cagccgacgg
     5281 ccttgatgtc gaactcgtcc ttcagtcggc ggaggcgacg gaggccctcg acgccggcgt
     5341 gaggatcgca ctcggccgac gcgaagaacc ggccggggaa gcggcgcatg gcctcctggc
     5401 tgatctcgtc gacgccgatc attccctggg cgatgcccac ccggtccatc gcgtcgacgg
     5461 tgacggcgac ggggtcgtcg gcatcgacga actccttctc gggcacttgc ttgaacatgt
     5521 actcggccgg gaactcgaac tcttccttcg actcgcggtc gcgcgtctga cgggtgatga
     5581 actcgtacac cgccttcatg tccccgtggg gaaagccgat catcgtgtcg atcgtgccga
     5641 tgccgtcggg gatggccatg tgcgagtacc tccggttgca ccgggctcgg aggagaccgg
     5701 tggacgagac tagaatcgca gtctagtttc gctccgatgt cgcccgctga tctgcccgtt
     5761 ccgatcgacc ccgagttcgc cgagatgctg gcgatgctgc ccgagatccg gatcaccgcc
     5821 gagacgttgc ccgagttccg cgacctgctc gccgccctcc agacgacccc gccggtcgac
     5881 ggggtgaccg ccgaggagat cgacgtcgac gaccacgtgt cggtgcgcgt gctccgggac
     5941 ggccggaccg aaccgggcgg agcggatgac gtggacggag cggcgcggcc cgccgtgctg
     6001 tggatccacg gcggtggcta cgtgctcggc caccacgcga tggacgacgg tcacctcggc
     6061 ccgtgggtcc tcgagctcgg gtgcatcgtg gtatcggtcg actaccggct ggcgcccgag
     6121 cacccctacc ccaccccgct cgaggactgc tacgccgcgc tgtggtggct gcaccagcac
     6181 gccggcgacc tcggggtcga ccccgaccgc atcggcgtcg ccgggctcag cgccggtggc
     6241 ggtctcgccg ccgcgctcgc agtgctggcc cgcgatcgcg gcgagctgcc gatcgccttc
     6301 cagttgctcg acagtcccat gctcgacgac cgccgcatca ccccgtcgag caactacgac
     6361 gggctcccgg tgtggaatcg ccactccaac gagttcggct ggaaggcgat gttgggcgac
     6421 ctcgccggtc gcgatgacgt cccggcgacc gcgtcgccgt cgcgggcgac cgacctgtcg
     6481 ggcctccctc cggcgttcgt cgtggtcggc ggggccgacg gcttccgcga cgaggacatc
     6541 gactacgcgg tgcgcctcca acaggccgga gtcccgaccg agctgcacgt gtttcccggg
     6601 gcaccgcacg ggttcggatt gttcgtgggt tcgtcgatcg cccagcaggc cgaggcggct
     6661 cagcgcaact ggttggcccg ccagtggggt tcgtcgtgac ccgcacctac ctcatcacgg
     6721 gttcggcgtc gggcatcggg cgcgccacgt tcgagttgct gaccggccag ggccaccggg
     6781 tcatcggggt cgatctccac gacgccgagg tcgtcgccga tctcgccacc gccgccgggc
     6841 gcgccgcgat ggtcgagcgg gtcaacgagc tgaccgacgg ggtgctcgac gctgtcgtcg
     6901 ccaacgccgg ggtgcaggac acgcccgagg gctgcgtgcg cgtcaactac ttcggcgcgg
     6961 tcgccaccct cgaggggctc cgaccgctgc tcgcgagagg cacggctccc cgggcggtgg
     7021 cgacgtgctc gtcgtcggtg ctcaacgaga actccccccg actcgtcgcc gctctggtgg
     7081 ccggtgacga ggaggacgcc gtggcggcgt cggtgaacac cggcctcgcc gcgtacccgt
     7141 cgtcgaagcg ggcgctcgcc cgctggatac gacgcactgc cccgacgtcc ggctgggccg
     7201 gggtcgggat cgggctcaac gcggtggcgc ccggcgtcgt cgagacggcg atgacagcgc
     7261 cgctgttggt cgacccggcg atggtcgagc tgatcgaggc cgccgtcccc atgccccacg
     7321 gaggcatcgc acaaccgggg catgtggccg atctcatcgc ctacctcacc tcgccgaaca
     7381 gcgcccgcat ctgcgggcag gtcgtcttcg tcgacggcgg cgccgacgtg gtgctgcgcg
     7441 gcgaggacat ctggtgaact tcgacgacag tccggaggag cacgcgttcc gcctgaaggt
     7501 tcgggcgttc ctcgacgagc acgccacgtt gcgcaccggc accgaggccg actggtggag
     7561 cggcccgacc gacgagtccg acgagtccga tctcgccttc cttcggaggt gtcaggactg
     7621 ggcgcgcacg gtgtacgacc acggatgggc tggcatcgcc tggcccacgg cgttcggcgg
     7681 acgcggcgag agcgtcgccc agatgatcgt gtgggccgac gaggtgtcgc gttacgacgt
     7741 gaccatcggg ttcgtcggga tcgggcacgg gctcgtgggc ccgcccctga tgcggcacgc
     7801 gacccccgag cagcagcagc gctacctcgc tccgctgttg cgcggcgacg agatctggtg
     7861 ccaactcttc agcgagcccg gcgccggttc ggatctcgcg gcgctgtcga cccgcgccga
     7921 actcgacggc gacgacgtcg tggtctcggg ccagaaggtg tggacgtcgg aagcccacca
     7981 cgccgacttc ggcatcctgc tcgtccgcac cgacccgacc gctccgaaac accacgggat
     8041 cacgttcctg atcgtcgaca tgcgcagccc cggcatcgag gtgcggccgc tgcgccaggc
     8101 gagtggccag gcccatttca acgaggtgtt cctcgacgag gtccgcatcc ccgtggccaa
     8161 cgtggtcggc gagatcgacg gcgggtggaa ggtcgcccgg accgtgatgg cgagcgaggc
     8221 cggcatgatc ggcaccggcg gcaacgaccc ggcgaactac gactcgatgc tgcgcctcgc
     8281 ccggggcctc ggacgcaccg ccgacccact cgtccgccag ggcctcgccg acctccacgc
     8341 ccgcgagcag atgatccggt tcatggggat gcgcttccag acgttcctca ccaccggccg
     8401 gggcaccccg cccgatccgt cggtgctcaa gaacttccac acccgcaaga acgcccgcaa
     8461 ggccgatctc gggttggcga tccaaggcgc accggggctg ttggacggcg ccgacgcgtg
     8521 ggaacacggc ctgtggcagc gctcgatggt gatgcagttc gcctcgagga tcggcggcgg
     8581 caccgacgag gtgcaccgca acatgatcgc cgagcgggca ctcggcctcc cccgcgatcc
     8641 ccgctgatcg cccgtcactc cccgtcaact tccagttgcc gcacggcctg gcgctccacc
     8701 agggtggtcg acatgaccga accgacgccc ccgaccccga ccgttcgtct cgatgacctg
     8761 atcgacgccg ttacggccgt tcaccccgac gaactccagc aactgtcggg cgcagtgctc
     8821 gccgccgacc gcctcgggga actcgctgac cacctcgtcg gacacttcgt ggaccgggcc
     8881 cgccgctcgg gcgcctcgtg gagcgagatc gggcggagca tgggcgtgag caagcaggcg
     8941 gcgcaacagc gcttcacccc ccgctccggc gccgaccgcg gtgtcgatcc gtcgcagggt
     9001 ttcgagcggc tcaccacccg ggcccgcaac gtgatcgtcg ccgcccacga cgtcgccgtg
     9061 gcgaggtcga acgtcgaggt gacgcctgca cacctcgtgc tcggtctcct cagcgaaccc
     9121 gacgcgctcg ccggggtcgc gctccaggcg cagggcatcc cgttcgaagc ggtgcgggcg
     9181 gcggcgatgg cggcgctgcc gggtccggtc tcggagcctc cggcgctcgt gccctacgac
     9241 gccgcggcca agaaggtcct gcagctgacg ttccgcgagg cgctgcgtca cggacacagc
     9301 cacgtcggaa ccgagcacct cctgctcgcc ctcctcgagc acgaggacgg ttcggggccg
     9361 ctcaaccggg ccggcgtcga caaggccgcc accgaggtga gcatcgccga cgccctggca
     9421 tcgaggccgg cccgtccccc tgcctcgccg tccgaggcgc cgctggccta gcgtgccggc
     9481 ccatgccgat ccccgcgttc cgactgctcg ccctcaccga agccgtctcg tggctgctcc
     9541 tcatcggagc ggcggtgctc aaacgggcgg cggacctccc ggaagcgacc cagatcatgg
     9601 gaccgatcca cggtgtgatc ttcctcgtct acgccggcgc cgtgctctac gtgcgcgacg
     9661 acctgaagtg gccggccggc cgcaccctca tggcgctgtt cgccgcgctg ctgcccctcg
     9721 gcacctatct catcgtcgag cgccgctacc tcgccgaagc ccgggtcgcc gacctcgacg
     9781 ccgccggcgc ctgaggcgga ccggccggac tcgacctcga ctgggtttgt tcggggtcgc
     9841 gagcccgcac gtcgtctcct cagccagatg acgacctgga gagccgcagg tgcgggaggt
     9901 ccggccgggg accagcgagc aactcctcga ccgcgaccag gtcgaggtgg tccgcaacga
     9961 ggtcacccag tcgatcgagt cgggcggcac gctcggcggc gaacgagatg tcggatgcca
    10021 ccacgaaccg tcggccacac gcgtgggcga cctcggccag gtagcggcgg cggaacgcgt
    10081 cgttctcgag cacaccgtgc cacacggtgc cggcgacggc accgacccgg cagccctcgc
    10141 ccgacccgtc ggccgccgcg acgaacggtt caccacccga gctcccgacg atgccgtggt
    10201 ggatctcgta cgcctcgatc accgaaccgt cggccaggac catcgacggt cgggccagca
    10261 ccttctcgag cccgaacgaa gtgtcgaccg gcaacaagcc gagaccggcg acggtccccg
    10321 cacccgactc gacaccgtcc tcgatcgacg acccgagcat ctggtagccg ccgcagatgc
    10381 cgagcaccgc tcgtccggca gcggcgtggc gagcaacggc gatgtcgatg cctcgctcgc
    10441 gcatccacgc caggtcgctg acggtggcac gcgtgccggg caggacgacc agatcggcgt
    10501 cgacgagctg cgccggctga tccacgaagc ggatcacgac acccggttcg agcgccaggg
    10561 cgtcgatgtc ggtgtggttg ctcatgcggg ggaaggccac gacgttgacc cggagcacat
    10621 cgtcaccgag cggcgccgga ccggaacgga gcagctcgaa gtcgatggcg tcctcggcgt
    10681 cgaccccgag accccgcacg aagggcaaca ccccgagcgt cgggcgtccg gtcaggtccg
    10741 ccagcatgtg gaggcccggc tcgagcaggg acacatcgcc acggaacttg ttgacgacga
    10801 acccggcgat caacgcctgg tcggcggcgt cgagcacggc cagcgtgccg acgaacgatg
    10861 cgaacactcc cccacggtcg atgtcgccga cgagcagcac cggcgtgtca gtggcgcggg
    10921 cgaaccccag gttgacgatg tcggagcgcc gcaggttgat ctcggccggg ctgccggccc
    10981 cctcgcagat gacggcgtcg aagcgggccg ccagatcgcg gtacgcgtcg acgacgacgt
    11041 cgagcaggga gctctgggcc acgggaccgt cgtcacccca gaacgtggac gcatcacgct
    11101 cgccgacggc ccggcccatc acgatcacct ggctcgacgt gtcactcccc ggcttgagca
    11161 gcacgggatt catcgccgcc tcgggctcga caccggccgc ccacgcctgg gccgcctgcg
    11221 cccgaccgat ctcgagcccg tcggcggtca cgaacgagtt gagcgacatg ttctgcgcct
    11281 tgaacggcgc gacccgcacg ccctgccggg cgagggcccg acacaggccg gcgacgacga
    11341 tcgtcttgcc gacgtccgac gccgtcccgc agacgagcag gccggcgccg gtcaccgcgt
    11401 catttgtagc ggaggacgcc gcggatgttc ttgccggcga gcatgtccgc gaacccctcg
    11461 ttgatgtcct cgaggtcgta ctctcgggtc accatgccgg ccaggtcgag ctgaccgctg
    11521 cggtacaggt gcatgaggcg cgggatgtcg gagttggggt tggccgagcc gaagatcgtg
    11581 cccaccagct gcttctccat caacgtcaga tcgcagagcg agatgttgat cgagccctcc
    11641 gacgccgggt ggatgttggt cacgaccaca cgaccgcgct tggcgcacag ctgcatgatc
    11701 gggccgatct gctcgccgtc gcccacaccg acgcagcaga tgatcttgtc gcacatccgg
    11761 ccccaggtga tctcctggat gagcgagaac gcctcgccga tgctgttggc cgtgtgcgtg
    11821 gcaccgaact tcatcgcctg ctcacgcttg aactcgacgg ggtcgatggc gatgatgttg
    11881 cgcgccccgg ccaggcgggc accctgcacc gcggcggcgc cgataccacc gacaccgatg
    11941 acggccacgt tgtcgccggc gcggacctcg gcggcataga ccgacgagcc ccacccggtg
    12001 gtgacgccac agccgaccag gcaggcgagc tcgaggggga tgtcgtcctc gatcttcacg
    12061 caggacatct cgttgacgac ggtgtgcttg ccgaacgtgc cgaggcagac catggtccac
    12121 aggtcggtgc cgtcggcggc gtggtggcgg ctggtgccgt cgagctgggc accgacgagc
    12181 aacgccgcgc cgttgtcaca caggttcgaa tggccgtcgg cgcagctctg gcagatacca
    12241 cacgccggaa cgaagctgaa caccacgtgg tcgccgggct tcaccttggt gacgccgtcg
    12301 ccgacctcca tgacgatgcc ggcaccctcg tggcccccga tgagcggcgt gacggccggc
    12361 aggtcaccgg tgcgggcgtg atcgtcggag tggcacatgc cggaggcgac catctcgacg
    12421 aggacctcgc cggccttcgg cgggtcgagc gtgatcgtct cgacgctcca cttgccaccg
    12481 ggttcccaca tgatcgcggc ttgggtctcc actgctactc ctggccggcg gcaaccttcg
    12541 ctaggcacgc acccgactcg gccgggtgct gctaacgctc cggtacctcc ggcagaccgc
    12601 cgctagcagt gagcaaggta catcatctgc gccggcacat ctgacgccgc gtcaggactc
    12661 cgagccaggg cgaaatgggg tcgtgccgac tgagccggac cgcatcgagc ctccacggga
    12721 cttccatgcc atgtcgcaac ctttcacggc tttacattcg acatcgcacc ggccagactc
    12781 cggtcgcatg tgggagacga cggcgcaggg aaccgttcgg gtcgggacgg atctgatgtc
    12841 ggtcagcgag gtcgcctcgt cgatcgatcg gttcggcgac cgctacctcc ggcgggtgtt
    12901 caccccggcg gaactggccg cctgcgccgg gcccagcggc ccgtcggcgg cgcgactcgc
    12961 cgcccggttc gccgccaagg aatgcgccgt gaagatcctg cacccgatcg acggggtgaa
    13021 cttccacgac atcgaggtgc agaccgaccg tgacggtcga ccctcgatcg cgttccacgg
    13081 caccatggcc gaccgcgccc gagcgctggg tatcgtcgac gcctcgctca gcctgtcgga
    13141 ggagcggggc ctggccaccg ccatcctcgt tgcggtcatc gcgcccgctc cacgactccg
    13201 ctgacgacga ggccgagaac g
//
LOCUS       DAOSOA010000054        13188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4728_length_13188_cov_6.705246, whole genome shotgun sequence.
ACCESSION   DAOSOA010000054 DAOSOA010000000
VERSION     DAOSOA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13188
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4728_length_13188_cov_6.705246"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..773
                     /locus_tag="PKY13_05395"
     CDS             45..773
                     /locus_tag="PKY13_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79398.1"
                     /translation="MTSSRGAGGARPARVRVRPIALVAAMIAVFSLVVGCSGDDAAKD
                     ADRRSGSPSAAGGSSSGGSGSAASTGEAEQAAAEELAAAQAASKKPSGVAGVPAPNDD
                     DGARNLAAPDPSSGPSADDTSGSVARDPLASPSPTFPQPGRPDVDGLQPLDGCDEISA
                     PFYRAVSTLNVTSSPSTIVDGVKDLTTVAERAQAPIAAAVQTLLTTAQSAAADAARSQ
                     DFASEAFQNAVKVVHVYLGDACGR"
     gene            882..>2005
                     /locus_tag="PKY13_05400"
     CDS             882..>2005
                     /locus_tag="PKY13_05400"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HOT79399.1"
                     /translation="MTNIQDVTNGSGSGRRRSPAVIVSVLLIASLVGVGIVQLQQDDK
                     VEAAIVVGDGTISEHAGASCWSIKQSYPNSPDGTYWVQTPALIAPMRVYCDMTTDGGG
                     WVLVGRGRDGWNWPSTGQGVQSAIATTPSGTDAFGVAAYSNKIIDGLLNNTRPDSLDD
                     GIRLRRATNNTGTSWQDIRWKLEGPRPGFGGRDKWTWAFGAGISLDSVSINGVSYGKA
                     NTYFSGSNLTGQTGASPNLTGVNAWMTYNNNFPNRRGGFGFNSNTGGNTAATSYWYYN
                     GTRTVPFTQVWLRPKISEAAQPAIPATGTPESTVRKFLKSNPEALNWGVTDNVDPTDQ
                     FPDLNSLVMGFAQIGDTVYVGGEYQNVQNGTGATKIAQPYL"
     assembly_gap    2006..2105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2106..4757
                     /locus_tag="PKY13_05405"
     CDS             <2106..4757
                     /locus_tag="PKY13_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOT79400.1"
                     /translation="ALTRTMAIKGNWLYVGGYFTGISGGVASPATMGAANIVRVSIED
                     GRPDTTWLPTVSANLFHLEPSPSSDRVWLAGNFVSVNGQFARRIAAVSTVDGSRADPA
                     NMQQWQPSQPLADPTQPSECCEYQYVIHEEGNSIWNAGSQHNITKSSLSTYTQERYSE
                     TAAGGDFQGMTSQNGIIYAGCHCTNFTWTDQDIYAHPSQYPKATQADAINSFGAWDAV
                     TGEYITEFYPSIYTTVGEGPWEFFNDNAGCMWVGGDFDRGGWNGTGFDYVGGFAKFCN
                     RDTTAPSVPTNLALTTNGGRAQLTWTASTDNSPTSIRYEILRDDRVIATVWGTYYLDP
                     DSSAGSNYFVRAVDETNNRSASTAAKNFFVQQPPATLVAAADTWRYLDDGSNQGTAWR
                     LPSTSVATWPTGVAPLGKALSPTTAIPTTGITQYFVKDFNVANPASIATLRLTLRADD
                     GAVVYLNGNEIARHNMPFGSPLFSTPAWAAQTVSPFDLNFDLPKDGLVAGPNRLAVEL
                     HQSAAADVDSIFSATLIGNAPTTDVIAPTAPTVTAPAINNTSVRVDWTASTDNVAPVA
                     YRLQRNGVTISYRNAGSTLTFTDQQLVPGTAYTYTVKAIDAAGNESPEGSVVATTTGG
                     APVPIGVVSPSTWRYESSGTDQGTAWRLPATSLASWSTGLSPLGKALGETTAIPATGV
                     TQYYAQDFNVASLTGIGTLLLQFRADDGAVVYLNGNEIARTNMPFGPPGFLTTAWAAQ
                     VGQPGLQTVALPKDALVQGTNRLAVEVHQAVANDTDAFFDATISGVSTSTDVTAPTSP
                     GALTVTERTPTAIRMTWTASTDNVGVVVYRLKRDTTTIAYRQASSSLTYFEQQLTRGT
                     NYTFTVVAVDAAGNESPVATVATSTLP"
     gene            complement(5135..>6595)
                     /locus_tag="PKY13_05410"
     CDS             complement(5135..>6595)
                     /locus_tag="PKY13_05410"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT79401.1"
                     /translation="KRPLSSSLDGSIDVAIILELVIYALVLGYLVMFVVTPPRWRRPT
                     SVQFMLRGYAALMCVSVVYGLYPQLGAVRAVQLVIMVVFAAALANRASRRQMHQLAHG
                     YLALITMSVFLGLIWRVPFSINQEHRFNWMHVHSVIAAAMLALAVVIATAFLIRPRVA
                     SAGVPHMSRGIYGFCLVVCTIALIATETRGSLAGAVGGIGVVVFFTVPRRERLPIGVI
                     GLLGVGTAVAVFINPILEYLSRGESTESITTLSNRTLLWDIAFQLVGERPLTGYGLTA
                     SRGVFFEAIHLGGAHNAFVNVLVDGGIVAAGLWLGVIVTIGIAIRNAARRRDVDTALL
                     AGLLVTLMVNGLTTEGVGSGSGVSALWLFILAGWVGVMQREWAAHRRSPRVVHRNVVQ
                     RTPVRPRSTHPTAPTGDRRRAAIAAAARPTAIGPGPRPEATTALPAGSPAPKALPASP
                     DRPKALEPGTRPGATSDTAPAPGTQRRGKHFRPPVD"
     assembly_gap    6596..6695
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6696..7802)
                     /locus_tag="PKY13_05415"
     CDS             complement(<6696..7802)
                     /locus_tag="PKY13_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79402.1"
                     /translation="MAVRDHLRSGSAALVSQGITAGSSLVLQVLALWQLGKSGLAFFA
                     ILSAGIMVTFNAVHTGLIGDALSVFDRHEPGIRRALVKTSAVSAGASLFVGFFGALLF
                     AERSALSSGLFALALLAWTVEETGRRILMARLEFWKLVVNDAVFAVASLATVGVILVI
                     GDLSIEWLVASMLIGSIASIADAVRQLPREELELGPKAPAEMRRLIEFSGWRAGQIGI
                     RPLSLLVSRIVIVGVASPASLGLLEAARLLIAPVLTAANGLGVFLLPTFAKRAKSDDD
                     LGEMWSIAALSAVAAAVLGAVSVIATPTVSRLLGQDPAIPTTLVASWVVYSIAYCANI
                     PVVNALVARREARSLFNGRLVDAAIGVALSYVLTS"
     gene            complement(7805..9307)
                     /locus_tag="PKY13_05420"
     CDS             complement(7805..9307)
                     /locus_tag="PKY13_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79403.1"
                     /translation="MFASEPTTPEQAPGIIEATLRYRSITLAVTLAGLILAAFFTLAQ
                     PNDAMAEGRLVLTDPRGNTVFHDGNSFQVDLTRYISERSDFASSGSVLDRARELAPSA
                     GSTTQDVRDACQVSPEDTGNVLVIECSYANEKTARSVVDAIATAYRELTTAQTADKAG
                     AAIDALDGERTELDKLIEESRDAATDDPYGNALAQSAGDRLTQLELRASDIRTAQALF
                     GDGTESYDAARVPPSTGLLTMLVRNSIVGALIGFMMAVLIAWFRADRSPIALVATDAA
                     NALGLPLLGEIEHDLIAGDGIDLVTVPEPSFQLVASNLGSVLNGGTVLFAPIHAIDGH
                     ADVVVKVALMAARAGKRVLLIDGDDANRSVSSLFGLANGPGLTEYVSGQAGDDAVTAR
                     IAFDTGPGMSQSALYLMGPGSPAGAMSALFRSSRTSEALRELEQMYDLILIGGPPWLE
                     SAGSTALGQVVDGVVVIVERGTSKESMENTRRQLAFLVSDTLGFVFIHDD"
     assembly_gap    9502..9601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9602..10333
                     /locus_tag="PKY13_05425"
     CDS             <9602..10333
                     /locus_tag="PKY13_05425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT79404.1"
                     /translation="AVVAVRRRSEARDRLVDRYIAPTAALRDRVVGGGIPADRVEVIP
                     HFVPDPGEPVPFDQRSRTLVYAGRLSEEKGIDVAVEAWSRLVDRPVDARFVVIGEGPL
                     EDELRARAVPGVEFLGRRPAAEVAATLATARLAVVPSVWSEPFGLFILEAMAAGLGVV
                     ASSSGGAPEFLADGAGWLVPPGDAAALAERLASLLAADDETDGLVRVAGEAARHRTLT
                     QYGGPEHLARLEQIYRVVRKIGAPK"
     gene            10380..10820
                     /locus_tag="PKY13_05430"
     CDS             10380..10820
                     /locus_tag="PKY13_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOT79405.1"
                     /translation="MATRGVALGSFDMFHVGHLRALRRAFELSDHLTVAVADDALVEA
                     RCGAAPLVPLVHRLEVLGGFGLAQEVVVVDSDLAAGLIEAHAVDHAFVTEVGPVVDEV
                     VGGTPRLPLTLIEVATTISGSLRNAPDQGLLWTPSGALDSVVCT"
     gene            10817..11557
                     /locus_tag="PKY13_05435"
     CDS             10817..11557
                     /locus_tag="PKY13_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT79406.1"
                     /translation="MTTYAEALEGLRSAQKPNYGVPAYSRFVNRPLGRQFAAVACVLG
                     LTPSQVTVISALVSATGIVLIATVDNTWVLGIGAAVALVLGYALDAADGQLARLTKAG
                     SAAGEWLDHSVDAVKISGLHLAVLVGLYRFADLADTAWYLAPIAFELVAVSRFSSMLL
                     IDQLRRSAPPGEQPLPTESSLLRSWAALVIDYGVLCLLFVTWGSVGLFLVLYTVMCVG
                     NWAYFVAASAKQYRELLGRTRVPAVAGP"
     gene            11554..11967
                     /locus_tag="PKY13_05440"
     CDS             11554..11967
                     /locus_tag="PKY13_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOT79407.1"
                     /translation="MTLVGYVPGVFDMFHVGHLRILQRSAERCDRLVAGIVDSAATRS
                     MKGADPLVDLEERLEIVSAVACVDDAVIDHAQSKLEVWERVRFDVLFKGDDWRGTPKG
                     DLLEAELATVGARVVYLPYTPETSSTHFRGQVNGS"
     gene            complement(12026..13021)
                     /locus_tag="PKY13_05445"
     CDS             complement(12026..13021)
                     /locus_tag="PKY13_05445"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT79408.1"
                     /translation="MNSTTMSTDGIVLVTGGGGFIGGHLVASLIADGYQVRSVDIKPF
                     DEWYQRHPEADNRVLDVSLLPDAMSAVEGTRYVFNLAADMGGMGFIENNKALCMLSVL
                     TSTHVLMAARDHGVERLFYSSSACVYAADKQTNPEITALVEADAYPAMPEDGYGWEKL
                     FSERMAHHFYEDYGLETRVARYHNVYGPNGTYDGGREKAPAAISRKVALAKLTGDHTI
                     DIWGDGLQTRSFMYIDDCVYGTRMLLESDIREPLNVGSSELVTINDLVAMTEKIAGVS
                     LKRTHDLSAPQGVRGRNSDNTRILELAGWEPSTSLQEGMERTYAWVYDQLVARGY"
BASE COUNT         2076 a         4335 c         4455 g         2022 t
ORIGIN      
        1 ctgatccgga cgattgctgt ggtgttgcag cgaggaggcc ggtgatgacc agttcccgag
       61 gtgctggcgg agcgcggccc gcccgcgttc gcgttcggcc gatcgcgctg gtggcggcga
      121 tgattgccgt gttctcgctc gtcgtcggct gctccggcga cgacgcagcg aaggacgccg
      181 atcgccgcag cggctcgccg tccgcagcgg gcggttcgtc atcgggcggc tccggttcgg
      241 ccgcttcgac tggcgaggcc gagcaggcag cggcagaaga actcgccgcc gctcaggccg
      301 cgtcgaagaa gccgagtggc gtggcgggcg tgccggcccc caacgacgac gacggtgcca
      361 ggaacctggc ggcccccgat ccctcgagtg gcccctccgc cgacgatacg tcgggctcgg
      421 ttgcccgtga tccgctggcc agtccctcgc cgacgtttcc ccagcccggg cgtcctgacg
      481 tcgacgggct ccagccgctc gacggctgtg acgagatcag cgctccgttc taccgtgcgg
      541 tctcgacgct caatgtgacc tcctcgccga gcacgatcgt cgacggtgtg aaggacctga
      601 cgaccgtggc cgagcgagcc caggccccga tcgccgcagc ggtgcagacg ctgctcacga
      661 cagcccagtc agcggcggcc gacgcggccc gctcgcaaga cttcgcctcc gaggcgttcc
      721 agaacgccgt caaggtcgtg cacgtctacc tcggcgacgc ctgcggccgc tgactccttg
      781 ccccctcggg cctctcgagt cccacggttt ccgccgtctc ggcgactcga ccttccggtt
      841 ccatgtgatg tcctcaccga agtgaacgaa cggtagtaga gatgacgaat atccaagatg
      901 tgaccaacgg ttccggttcc ggccgccgcc ggtctccggc ggtgatcgtg tccgtcctcc
      961 tcatcgcgtc gctcgtcggc gtggggatcg tgcagctgca gcaggacgac aaggtcgagg
     1021 cggccatcgt cgtgggcgac ggcacgatct ccgagcacgc cggagcgtcg tgttggtcga
     1081 tcaagcagtc gtacccgaac tcgccggacg ggacctactg ggtccagacg cccgccctga
     1141 tcgccccgat gcgggtgtac tgcgacatga cgaccgatgg cggtggctgg gtgctcgtcg
     1201 ggcgcggccg tgacggatgg aactggccgt cgacgggcca gggtgtgcag tccgccatcg
     1261 cgacgacgcc cagcggcacc gacgcgttcg gcgtcgccgc gtactccaac aagatcatcg
     1321 acggcttgtt gaacaacacc cgtccggact cgctcgacga cggcatccgc ctgcggcgcg
     1381 ccaccaacaa caccggcacg tcgtggcagg acatccgctg gaagctcgag ggcccccggc
     1441 ccggcttcgg tggtcgcgac aagtggacgt gggcgttcgg cgccggcatc tcgctcgact
     1501 cggtctcgat caacggcgtg tcgtacggca aggccaacac ctacttctcc ggttcgaacc
     1561 tgaccggcca gaccggtgca tcgccgaacc tcaccggcgt caacgcctgg atgacctaca
     1621 acaacaactt cccgaaccgc cgcggcggct tcggtttcaa ctcgaacacc ggcgggaaca
     1681 ccgcagcgac gtcgtactgg tactacaacg gcacgcgcac ggtcccgttc acccaggtct
     1741 ggctccggcc caagatctcc gaggcggccc agccggcgat cccggcgacg gggacgcccg
     1801 agagcaccgt ccgcaagttc ctgaagtcca accccgaggc cctgaactgg ggtgtgaccg
     1861 acaacgtcga ccccaccgac cagttcccgg atctgaactc gctcgtcatg ggcttcgccc
     1921 agatcggcga caccgtgtat gtcggcggtg agtaccagaa cgtccagaac ggtacgggcg
     1981 cgaccaagat cgcccagccc tacctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnngccct gacccgcacg atggcgatca agggcaactg gctgtacgtc ggtggctact
     2161 tcacgggtat ctccggtggt gtggcgagcc cggcgaccat gggggcggcg aacatcgtcc
     2221 gggtgtccat cgaggatggc cgccccgaca cgacctggct gccgacggtg tcggccaacc
     2281 tgttccacct cgagccctcg ccgtcatcgg accgtgtgtg gctcgccggc aacttcgtga
     2341 gcgtgaacgg tcagttcgca cgccgaatcg ctgcggtcag cacagtcgac ggatcgcgtg
     2401 ccgatcccgc caacatgcag cagtggcagc ccagccagcc gctggccgat cccacccagc
     2461 cgtccgagtg ctgcgagtac cagtacgtga tccacgaaga aggcaactcg atctggaacg
     2521 ccggatcgca gcacaacatc acgaagagca gcctctcgac gtacacccag gagcggtaca
     2581 gcgagaccgc tgccggcggt gacttccagg ggatgacctc ccagaacggc atcatctacg
     2641 ccggctgtca ctgcacgaac ttcacctgga ccgatcagga catctacgcc catccgtcgc
     2701 agtaccccaa ggcgacccag gccgacgcca tcaacagctt cggcgcctgg gatgcagtga
     2761 ccggcgagta catcaccgag ttctacccgt cgatctacac gaccgtcggt gagggtccgt
     2821 gggagttctt caacgacaac gccgggtgca tgtgggtcgg cggtgacttc gatcgcggtg
     2881 ggtggaacgg gacgggcttc gactacgtcg gcggcttcgc gaagttctgc aaccgcgaca
     2941 ccacggcacc gagcgtcccg accaacctgg cgctgacgac caacggtggc cgcgctcagc
     3001 tgacgtggac cgcatccacc gacaattcgc cgacgtcgat ccgctacgag atcctccgag
     3061 acgaccgggt catcgccact gtgtggggca cctactacct cgaccccgac tccagcgcag
     3121 gttcgaacta cttcgtccgt gcggtcgacg agaccaacaa ccggtcggcc tcgactgctg
     3181 ccaagaactt cttcgtgcag cagccgccgg cgaccctcgt cgccgctgcc gatacgtggc
     3241 ggtacctcga cgacggctcc aaccagggca cggcgtggcg tctcccgtcg acgtcggtgg
     3301 cgacctggcc gaccggggtc gccccgctcg gcaaggcgct ctcgccgacc accgcgattc
     3361 ccacgacggg gatcacgcag tacttcgtga aggacttcaa cgtcgcgaac ccggcgagca
     3421 tcgccacgct tcgcctcacg ttgcgggccg acgacggcgc cgtggtctat ctcaacggca
     3481 acgagatcgc ccggcacaac atgccgttcg gctcgccgct gttctcgacg ccggcgtggg
     3541 cagcgcagac cgtcagtccg ttcgacctga acttcgacct tccgaaggac ggcctcgtcg
     3601 caggccccaa ccgccttgct gtggagctcc accagagcgc tgcagcggac gtcgactcga
     3661 tcttctcggc gacgctcatc ggcaatgcac cgacgaccga tgtcatcgcg ccgacggcgc
     3721 cgacagtgac ggcaccggcg atcaacaaca cgtcggtgcg ggtcgactgg acggcgtcga
     3781 ccgacaacgt tgcccccgtg gcgtaccgcc tgcagcgcaa cggtgtcacg atcagctacc
     3841 gcaacgccgg cagcacgctc acgttcaccg atcagcagct cgtgcccggc acggcctaca
     3901 cctacaccgt caaggcgatc gacgccgccg gcaacgagtc accggaaggt tccgtggtcg
     3961 ccacgaccac cggtggtgcc ccggtgccga tcggcgtcgt gtcgccgtcg acctggcgct
     4021 acgagtcatc gggcaccgac cagggcaccg catggcgcct gccggcgaca tcgctcgcca
     4081 gctggtcgac cggcttgtcg ccgctcggca aggcgttggg tgagacgacg gccatcccgg
     4141 ccaccggcgt gacccagtac tacgcccagg acttcaacgt tgcgtcactc accgggatcg
     4201 gcaccctgct gctgcagttc cgggccgacg atggcgccgt ggtctacctc aacggcaacg
     4261 agatcgctcg caccaacatg ccgttcgggc ctccgggctt cctgaccacg gcatgggctg
     4321 cgcaggtcgg ccagcccggt ctccagacgg tggcgcttcc gaaggacgcc ctcgtgcagg
     4381 gcaccaaccg gctcgccgtc gaggttcatc aggcggtcgc gaatgacacc gacgccttct
     4441 tcgatgcgac catctccggc gtctcgacga gcaccgacgt gaccgcgccg acgtcccccg
     4501 gggccctgac ggtcaccgag cgcacaccga ccgccatccg gatgacgtgg acggcgtcga
     4561 ccgacaacgt cggcgtggtc gtgtatcgac tcaagcgaga cacgaccacc atcgcctacc
     4621 ggcaggcctc gtcgtccctc acgtacttcg agcagcagct cacccgcggg acgaactaca
     4681 cgttcaccgt ggtcgcggtg gatgcggccg gcaacgaatc gccggtggcg actgtcgcca
     4741 cctcgaccct gccgtagccg gcgacacccc gagcaccgat ttcgaacggc aactcgagcc
     4801 tcacccgtgc ggatccgcac gggtgaggct cgagttcgtc accccacggc cgcagggcga
     4861 ggccggcgtc aaacggcgtt gtgcgccaga tcaccagggg tgcccgccag cggcacgtcg
     4921 agcccgcgcg ctgccacggg gccgcatcga ggaggagccg gtggtcgagg atgttgtggt
     4981 tccagcgcgc cgactcggcg atgtcaccct cgacattcga aacgcgtccg tcggacgcgt
     5041 cggcaacacc ggcgacgggt tggagcgcct gcgatcgcac gggtgaaacc cggtcatgcc
     5101 gacccggccg ctggccgggc gggggccctg ggcgtcagtc gacgggcggt cggaagtgtt
     5161 tgccccggcg ctgggtgccc ggcgctggtg cagtgtcgct cgtcgctccc gggcgggtgc
     5221 cgggttcgag cgccttcggc cgatccgggc tcgcagggag tgccttgggt gccgggcttc
     5281 cggcaggaag cgcggtcgtc gcttcggggc gagggcccgg tccgatggcg gtaggtcgag
     5341 ccgctgccgc gatggcagcc cgtcgtcggt cgcctgtcgg agcggtcggg tgcgtgctcc
     5401 gtgggcgcac gggtgtgcgt tggacgacgt tgcggtggac cacccgtggc gagcggcggt
     5461 gagccgccca ttcgcgttgc atcacgccga cccagccagc gaggatgaac aaccagagcg
     5521 ctgagacccc cgagccggac ccgacccctt cggtcgtgag gccgttcacc atcaacgtca
     5581 cgagcaggcc ggcgagcaac gccgtatcga cgtcacggcg gcgggcggcg ttgcggatgg
     5641 cgatcccgat cgtgacgatg acgccgagcc acaaccccgc ggcgacgatg ccaccgtcga
     5701 cgagcacgtt cacgaacgcg ttgtgagcgc caccgaggtg gatcgcctcg aagaagacgc
     5761 cacgcgatgc cgtgagtccg tatccggtca agggccgctc gccgacgagt tggaacgcga
     5821 tgtcccacag cagcgtgcgg ttcgacagcg tggtgatcga ctcggtgctc tcaccgcgcg
     5881 agaggtactc gaggatcggg ttgatgaaca cggcgacggc cgtgccgacg ccgaggagac
     5941 cgatgactcc gatcggaaga cgttcacgtc gcggcaccgt gaagaagacc acgacaccga
     6001 tgccgcccac agctccggcg agcgatccgc gggtctcggt cgcgatcagc gcaatcgtgc
     6061 agacgaccaa gcagaacccg tagatcccac gggacatgtg cggcacccca gcgctggcga
     6121 cgcgaggccg gatcaggaac gcagtagcga tcacgaccgc gagcgcgagc atcgccgcgg
     6181 cgatgacgga atggacgtgc atccagttga agcggtgttc ctgattgatc gagaacggca
     6241 cgcgccagat gagcccgagg aacaccgaca tcgtgatcag ggccaggtag ccgtgcgcca
     6301 gctgatgcat ctggcggcgg ctcgcccgat tggcgagcgc tgcggcgaac accaccatga
     6361 tcacgagctg caccgcccgc accgctccca gttgcgggta gaggccgtac acgaccgaga
     6421 cgcacatgag cgccgcgtac ccgcgcagca tgaactggac cgacgtcggg cgccgccagc
     6481 gcggtggcgt gacgacgaac atgaccaggt agccgagcac cagcgcgtag atgacgagct
     6541 cgaggatgat ggcgacgtcg atcgagccgt cgagggagct ggagagcggc cgcttnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgacg tgagcacgta cgacagggca
     6721 acgccgatgg cggcgtcgac gagccgaccg ttgaacagcg atcgggcctc gcgccgggcc
     6781 acgagcgcgt tcacgacggg gatgttggcg cagtaggcga tcgagtacac gacccagctg
     6841 gcgacgaggg tggtcgggat cgccggatcc tggccgagca aacggctcac cgtcggcgtc
     6901 gcgatcaccg acacggcgcc gaggaccgcg gccgcgacgg cgctgagcgc cgcgatgctc
     6961 cacatctcgc ccagatcgtc gtcggacttc gcccgcttgg cgaacgtcgg gaggaggaac
     7021 acgccgaggc cgtttgccgc ggtgaggacc ggagcgatga ggagacgcgc cgcctcgagg
     7081 aggccgaggc tggccggaga ggcgacgccg acgatcacga tcctcgacac gaggagtgag
     7141 agcgggcgga tgccgatctg cccggcgcgc cagcccgaga actcgatcag gcgtcgcatc
     7201 tcggcagggg ccttggggcc gagctcgagt tcttcgcggg ggagctgacg gacggcgtcg
     7261 gcgatcgacg cgatcgagcc gatcagcatc gacgccacga gccactcgat gctcaggtcg
     7321 ccgatcacga ggatgacgcc gaccgttgcc aacgaggcga cggcgaacac agcgtcgttg
     7381 accaccagct tccagaactc gaggcgggcc atgaggattc ggcgaccggt ctcttcgacg
     7441 gtccatgcga ggagcgcgag ggcgaacagg cccgaagaaa gggcggagcg ttccgcgaac
     7501 agcagcgcac cgaagaagcc gacgaacagc gaggcgcccg ccgacaccgc cgacgtcttc
     7561 acgagcgccc gacggatccc gggttcgtgg cggtcgaaca ccgacagcgc gtcgccgatc
     7621 aggccggtgt ggacggcgtt gaacgtgacc atgatgccgg cgctgaggat cgcgaagaac
     7681 gcgagcccac tcttgccgag ctgccagagg gcgaggacct ggagcaccag gctggacccg
     7741 gccgtgatcc cctgggagac gagcgctgcg ctcccggatc ggagatggtc gcgtaccgcc
     7801 acggtcagtc gtcgtggatg aagacgaagc cgagggtgtc gctgacgagg aatgcgagct
     7861 ggcgtcgcgt gttctccatc gactccttcg aggtgccgcg ctcgacgatc acgacgacgc
     7921 cgtcgacgac ctggccgagt gcggtcgagc cggccgactc gagccacggc ggcccgccga
     7981 tcaggatcag gtcgtacatc tgctccagtt cgcggagcgc ctcggacgtg cgtgacgagc
     8041 ggaacagcgc cgacatcgcc ccggcggggg aacccggacc catgaggtac agcgcggact
     8101 ggctcatgcc ggggccggtg tcgaacgcga tgcgggcggt gacggcgtcg tcgccggcct
     8161 gacccgagac gtactcggtg agacccggtc cgttggcgag accgaacagg ctcgacaccg
     8221 accggttggc gtcgtcgccg tcgatgagga gcacgcgctt gcccgcccgg gcggccatga
     8281 gtgccacctt gaccacgacg tcggcgtggc cgtcgatggc atggatcggt gcgaacaaga
     8341 ccgtgccacc gttgagcacc gaacccaggt tcgacgccac gagctggaac gagggttcgg
     8401 gcaccgtgac gaggtcgatg ccgtcgccgg caatgaggtc gtgctcgatc tcgccgagca
     8461 gcggcaggcc gagtgcgttc gccgcgtcgg tcgccaccag ggcgatcggg ctgcggtccg
     8521 ctcggaacca ggcgatcagc accgccatca tgaatccgat cagggcaccg acgatgctgt
     8581 tgcgaaccag catcgtgagc agccccgtgg acggagggac gcgggcggcg tcgtaggact
     8641 cggtgccgtc gccgaacagg gcctgtgccg tgcgaatgtc ggacgctcgg agctcgagct
     8701 gggtcagccg gtcgccggcc gactgggcga gagcgttgcc gtacgggtcg tcggtcgcgg
     8761 catcgcggct ctcctcgatc agcttgtcga gctcggtgcg ttcgccgtcg agggcatcga
     8821 tggccgctcc ggccttgtcg gccgtctgcg ccgtcgtcag ctcgcggtac gccgtggcga
     8881 tcgcgtcgac cacggaacgc gctgtcttct cgttcgcgta gctgcactcg atgacgagga
     8941 cgttgccggt gtcctcgggg ctgacctggc acgcatcgcg cacatcctgc gtggtcgacc
     9001 cggccgaggg ggcgagctca cgggcacgat cgagcacgga ccccgacgag gcgaagtccg
     9061 accgttcgct gatgtaccgc gtgaggtcga cctggaacga gttcccgtcg tggaagaccg
     9121 tgttgccccg ggggtcggtc agcacgagtc ggccttccgc catggcgtcg ttcggttggg
     9181 cgagcgtgaa gaacgcggcg aggatcaagc cggcaagggt gaccgcaagg gtgatcgagc
     9241 ggtaccgaag cgtggcctcg atgatgccgg gcgcctgttc gggggtggtg ggttcggacg
     9301 caaacactca gtcagtatcg gtccgagtcg cctggatgtg aggggcggcg gcgagattgc
     9361 cgagaggttg cccatcgcgg agactcagtg cgtgagagtc ctcctcgtcc actgccgcta
     9421 ccggtcgccg tcgggcgagg aagccgttgt cgaacgggag cggacgctgc tcgaggcggc
     9481 cggacacgac gtcgaactgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 ngcggtcgtc gccgtccgtc gacggtccga agcacgagat cggctcgtcg atcggtacat
     9661 cgcgccgaca gcggccctcc gcgaccgggt ggtcggaggt gggatcccgg ccgaccgggt
     9721 cgaggtgatc ccccacttcg tccccgatcc gggcgaaccg gtgccgttcg atcaacggtc
     9781 acggaccctc gtgtacgccg gccggttgag cgaggaaaag ggaatcgacg tcgccgttga
     9841 agcgtggtcg cgcctcgtcg accggccggt ggacgcacgg ttcgtggtga tcggtgaggg
     9901 cccgctcgag gacgagctcc gcgcccgggc ggtcccgggg gtggagttcc tcggtcggcg
     9961 gccggccgcc gaggtcgccg cgacgctggc gacggctcgc ctcgccgtcg tcccgtccgt
    10021 gtggagcgag ccgttcggcc tgttcatcct cgaggcgatg gctgccggtc ttggcgtcgt
    10081 ggcgtcgtca tcgggaggcg cgcccgagtt cctcgccgac ggggcgggtt ggttggtacc
    10141 accgggcgac gcggccgctc ttgcggaacg gctcgcgtcg ctgctggccg ccgacgatga
    10201 aaccgacggc ctcgtccgcg ttgccgggga ggctgctcgg caccgcacac tcacccagta
    10261 cgggggtccc gagcacctgg cgcggctgga gcagatctac cgcgtcgtga ggaaaatcgg
    10321 ggccccgaag tagaatgatg tcctcactgc ccgttggcga cggaggctct cagatcatca
    10381 tggcgacgcg aggtgtggcg ttgggctcgt tcgacatgtt ccatgtcggg cacctccgcg
    10441 ctctccggcg agccttcgag ttgtccgatc acctgaccgt ggcggtcgcc gacgatgcac
    10501 tcgtcgaagc ccggtgtggc gctgctccgc tggtgccgtt ggtccatcgg ctcgaggtcc
    10561 tcggcggatt cggactggcg caggaggtcg tggtcgtcga ctccgacctc gctgccggcc
    10621 tgatcgaagc gcacgcggtg gatcatgcct tcgtcaccga ggtcggcccg gtcgtcgacg
    10681 aggtcgtcgg cggtacgccg cggctgccac tgacattgat cgaggtggca accacgatca
    10741 gtgggtcgct gcgcaacgcc cccgatcaag ggctcctctg gaccccgagc ggcgcgctcg
    10801 acagcgtggt ctgcacatga cgacctacgc ggaggcgctc gagggtctgc ggtctgccca
    10861 gaagcccaac tacggcgtcc cggcatactc gcggttcgtc aaccgtccac tcggtcgtca
    10921 gttcgcggcc gtggcgtgtg tcctcggcct gacgccgtcg caggtcacgg tgatcagcgc
    10981 actggtgtcg gcgacgggca tcgtcctcat cgcgaccgtg gacaacacct gggtccttgg
    11041 catcggtgct gcggttgccc tcgtgctcgg ctacgccctc gacgcggccg acggccagct
    11101 cgcccggttg acgaaggccg gctcggccgc gggcgagtgg ctcgaccatt ccgtcgacgc
    11161 agtcaagatc tccggcctcc atctggccgt actcgtgggc ctctaccgct tcgccgatct
    11221 tgccgacacg gcgtggtacc tcgctcccat cgccttcgag ttggtcgcag tgtcgcggtt
    11281 ctcgtcgatg ctgctcatcg accagctccg tcgatctgca cctccgggtg aacagccgct
    11341 gccgaccgag tcgtcgctcc tccgttcctg ggcggcgctc gtgatcgact acggcgtgct
    11401 gtgcctgctc ttcgtgacgt gggggagcgt tggtctgttc ctggtgctct acaccgtgat
    11461 gtgtgtcggc aactgggcct atttcgtggc ggcatcggcg aagcagtacc gcgagctcct
    11521 cggacgaacc cgggtcccag cggtggcggg cccgtgacgc tcgtcggcta tgtccccgga
    11581 gtgttcgaca tgttccacgt cggccacctg cggatcctgc agcgttccgc cgagcgttgc
    11641 gaccggctgg tcgccgggat cgtcgacagt gctgcgaccc gctcgatgaa gggtgctgat
    11701 cccctggtcg acctcgagga gcggctggag atcgtgagcg cggtcgcatg tgtcgacgac
    11761 gcggtgatcg accacgccca gtcgaagctc gaggtctggg agcgcgtgcg cttcgacgtc
    11821 ttgttcaagg gcgacgactg gcgtggcacg ccgaagggcg atctgctcga ggccgagctt
    11881 gccaccgtgg gtgcacgggt cgtctacctg ccgtacacgc ccgagacctc gagcacgcac
    11941 ttccgcggcc aggtcaacgg ctcctgatcc gccccgccga caccgccgga gcggtgcggc
    12001 gacggcgggt cgagaactcg gtcgatcagt acccgcgggc gacgagctgg tcgtacaccc
    12061 aggcgtaggt gcgttccata ccctcctgca ggctggtgct cggctcccag ccggccagct
    12121 cgaggatgcg ggtgttgtcg ctgttgcggc cgcggacacc ctgcggggcg ctgaggtcgt
    12181 gggtgcgctt gagcgacacg ccggcgatct tctcggtcat agcgacgagg tcgttgatgg
    12241 tgacgagctc gctcgacccg acgttgagcg gctcgcggat gtccgactcg aggagcatgc
    12301 gggtgccgta gacgcagtcg tcgatgtaca tgaacgagcg agtctggaga ccgtcgcccc
    12361 agatgtcgat cgtgtggtcg ccggtcagtt tcgcgagcgc caccttgcgc gagatcgccg
    12421 cgggcgcctt ctcgcggcca ccgtcgtagg tgccgttggg gccgtagacg ttgtggtagc
    12481 gggcgacccg ggtctcgagg ccgtagtcct cgtagaagtg gtgcgccatc cgctcgctga
    12541 acagcttctc ccacccgtat ccgtcctcgg gcatcgccgg gtaggcatcg gcctcgacca
    12601 gcgcggtgat ctcggggttg gtctgcttgt cggcggcgta gacacaggcc gacgagctgt
    12661 agaagaggcg ttcgacgccg tggtcgcggg ccgccatgag cacgtgcgtg gacgtgagca
    12721 ccgagagcat gcacagcgcc ttgttgttct cgatgaaccc catgccgccc atgtcggcgg
    12781 ccaggttgaa gacgtagcgg gtgccctcga ccgccgacat tgcgtcgggg agcagggaca
    12841 cgtcgaggac acggttgtcg gcctcgggat gacgctggta ccactcgtcg aacggcttga
    12901 tgtcgacgct gcgcacctgg tagccgtcgg caatcaggct ggcgacgagg tggccgccga
    12961 tgaagccgcc gccgccggtg acgaggacga tgccgtcggt gctcatggtc gtcgagttca
    13021 ttgcaatttc tcctgggtgg gggtgccggt ggcggtcggc gtgatggaat ttctgggcac
    13081 gaggcgcggc cgccgccggg cgatgccgag gacgaatgtc ggaatgccca cggcgtagcg
    13141 tacgaagagg cgtctcggtt cctgaatgag ccgatagaac cattcgag
//
LOCUS       DAOSOA010000055        13106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4765_length_13106_cov_5.196460, whole genome shotgun sequence.
ACCESSION   DAOSOA010000055 DAOSOA010000000
VERSION     DAOSOA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13106
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4765_length_13106_cov_5.196460"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="cydB"
                     /locus_tag="PKY13_05450"
     CDS             complement(<1..778)
                     /gene="cydB"
                     /locus_tag="PKY13_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOT79409.1"
                     /translation="MELTTVWFILIAVLWVGYFFLEGFDFGVGMLLHPLGRTEVDRRV
                     MVNTIGPTWDGNEVWLLTAGGATFAAFPNWYATLFSGFYLPLFLILVALIARGVAFEY
                     RGKVDDDGWRKRWDLAIAIGSWLPAVLWGVAFANIVAGVPIDKAGDFTGTLFTLLNPF
                     GLLGGVVTASLFALHGANFLALKTEGEIHDAARAAARRIAPVTIVAGAVFLLWNQIER
                     GTGWTWATVLVAAVALLASAAMERADRDGWAFVATGVAITA"
     gene            complement(803..2254)
                     /locus_tag="PKY13_05455"
     CDS             complement(803..2254)
                     /locus_tag="PKY13_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOT79410.1"
                     /translation="MNTLDLARWQFGITTVYHFFMVPLTIGLALVVAWYQTRWYRTGD
                     EAYLRLTKFFGKLFLVNFAMGVVTGIVQEFQFGMAWSNYSRFVGDVFGAPLAMEALVA
                     FFLESTFIGLWIFGWDRLPKKVHLATIWLAAAGTTASAYFIIAANSWMQHPVGVKFNP
                     DTGRAEMVDIWKVLTNSTTLAAYPHVIAGALMTAATFVGGISAWSLLKRRDTLAEVDR
                     RGLRRALRGAIALTLVAGVGVAVSGDFQARLMFEQQPMKMASAEALCNTEQGPGLSVL
                     ALVGEEGADCNLRTWAIPKGLSLLATHTTDATIKGVNDLNTEYQAKYGPGDYRPSLFI
                     TYWGFRLMILFGAIAAFGAVAGLYLTRGGRDLPRWKWLPVTTIAVIATPFLGNVFGWI
                     FTEIGRQPWVVAPNPNGIPEVRLLTADAVSKVSAWTVGTSLVGFTLIYGLLAVVELRL
                     LARYIKAGPAGVMPDPDAEGSDDTEGQPLAFAY"
     gene            2473..3129
                     /locus_tag="PKY13_05460"
     CDS             2473..3129
                     /locus_tag="PKY13_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOT79411.1"
                     /translation="MTCQTSVARGPRTWSIAATIMVLAASMWSAGCANTATGGGGDGA
                     GARKLSGVVVEPVPDVSALSLPEASPAAEPFRFVADGGDLLLVYFGYTACPDVCPTTL
                     ADLKAALKQLGAPSKQVSIAMATIDPRRDTADVISGYLHSFFPSATALRTDDDAALRS
                     VTNAFGASYLTEYPNTGEPRVSHSAYLYVVDDQGHVVLAWPFGTTSADIALDLEQLLA
                     "
     gene            3170..3745
                     /locus_tag="PKY13_05465"
     CDS             3170..3745
                     /locus_tag="PKY13_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOT79412.1"
                     /translation="MKARSLALASVLTLAVLGASACGDDTTSDAASDETTTTLAAGPT
                     ISGAWARNSPMEATNGAAYLAITGGTSDDQLVSASVDSTIAGKVELHETVAAGDSGDG
                     SMGATTMGAPTTAAGDTMTATTLAPAMEMRPVEAVEVPAGKKVELKPGGYHIMLLDLV
                     QPLVVGDSFDLTLTFAEAGEQVVTVEVKDAP"
     gene            3742..4101
                     /locus_tag="PKY13_05470"
     CDS             3742..4101
                     /locus_tag="PKY13_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79413.1"
                     /translation="MIRRVARRAAFGIALVAFVVSSCGSPVRASKQYHYVIPEGTAGL
                     IATNRAPEIFPADLTVRVGDSITIVNEDDFDQQVGPYLVKAGTTMTQHFSSPGVLEGA
                     CTMSMGGTLSVTVLPAE"
     gene            complement(4104..4679)
                     /locus_tag="PKY13_05475"
     CDS             complement(4104..4679)
                     /locus_tag="PKY13_05475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT79414.1"
                     /translation="MDTRTNRHRHPRPAPTSVVALLVAALLGLTAVGCNSSDDTTSSS
                     GDDDTLAAVTVTPLADAPPLQLGSASKTPMVLNVWATWCAPCRKEMPAFDVTASKYEG
                     VVRFVGVNLGDTEVAAQQFVDETGVGFDQYLDPDSTSQVALSITTMPATAFIRADGTI
                     AATHNGALDEAELERLIADELGVPATTTTGP"
     assembly_gap    4912..5011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5221..5661
                     /gene="trxA"
                     /locus_tag="PKY13_05480"
     CDS             5221..5661
                     /gene="trxA"
                     /locus_tag="PKY13_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT79415.1"
                     /translation="MTKTVIECPACGVRNRVADDAGGRPRCSACHTDLPWLVDVGSAD
                     FDGVIERSALPVLVDLWAPWCGPCRAVAPALEQLAMDRAGALRIVKVNVDQAPEVSAR
                     LGVQGIPTMVLFFDGAELSRQVGAVPGPAIARWVDGALLGVKRA"
     gene            complement(5674..6117)
                     /locus_tag="PKY13_05485"
     CDS             complement(5674..6117)
                     /locus_tag="PKY13_05485"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT79416.1"
                     /translation="MARYRTSVRSPWLPADAYDVMKDLSRFDEWDPGITAAVLVSGEA
                     GAVGSTYDLTVKGIGGGTVMRYEIVDAVPGTSVEARSQTSTLESVDVITVVPDGDSGC
                     IVTYDADLRLRGPLRVVDPLLALLFRRIGDRAAAGLRQRLDGVAA"
     gene            complement(6217..7062)
                     /locus_tag="PKY13_05490"
     CDS             complement(6217..7062)
                     /locus_tag="PKY13_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT79417.1"
                     /translation="MVTTGSNRVVVGFDDSEGSRRAAAWAAAVGQHTGADVLLLGASM
                     PGTGDHVRLDEAMRAERRRLDPADGPGSAPVTTAVTDVAGGDPREVLTGSPHTHGATL
                     IVTGRAGHGSGPGLFHVGSVAEYLAHHTTVPLVVVPDDADVTFVRIVVGVDGSPASRA
                     AVAWCADLAAATGAAVTAVNVHEPFFEWSPKPDPEGWRRDAERRIRDDLAPELVDAGI
                     DLTTVARRGERPTDGILKAAHGADLLVIGLGPLGPLGVRAGGTAFRTLHGARCPIALI
                     PVPTG"
     gene            complement(7162..7968)
                     /locus_tag="PKY13_05495"
     CDS             complement(7162..7968)
                     /locus_tag="PKY13_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT79418.1"
                     /translation="MKRLADRVAVVTGAGSGIGRATSDALARAGCHLALVDISPTAVE
                     ETATTIRSLGRHASTHIVDVRDADALAALPAAVVDAHGACHILVNNAGVTSAGPFEDE
                     SLDDLHWIVDINVWGVVNGCRAFLPVLREADEAHIVNLSSMVGLLGLPQNASYSLTKG
                     AVRGFSEALRNELVTTNIGVTAVFPGAINTNITNSARGSQSGRLAELGRSRLAPLAMR
                     PPSAVAKRIVAAIEQDRGRAVVGPDAHLVSLWSRVAPGRAGLIGRLTARL"
     gene            complement(7965..9155)
                     /locus_tag="PKY13_05500"
     CDS             complement(7965..9155)
                     /locus_tag="PKY13_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029256809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOT79419.1"
                     /translation="MTESSTSTAFEPARLGPLTLRNRIIKAATFEGVMPDGAVSDELV
                     EFHRRVAAGGAAMTTVAYCAVSPGGRVQRNTMVMSDQIVPRLRRLTDAVHSEGALAAA
                     QIGHAGLVANTRSNRTKTLAPSTRLSPPAMGLVRGATHAELDEVVDQFGRAARVAVES
                     GFDAIEVHLGHNYLLSSFLSANLNKRSDEYGGSLENRMRLPRRAIAAVRAAVGTDVAV
                     TAKFNMADGVPQGLWLDESLPFAAMLEADGQLDALQLTGGSSLLNGMYFFRGDVPMAE
                     FIATQPAAVGLGLRVMGHKIFPEMPFEEGFFLPFARQFRAALSMPLILLGGINELATI
                     EGALAEGFDFVAMARALLRQPDLPNRMRDGEQSASLCVHCNKCMPTIFTGTRCVLTTP
                     EVNA"
     gene            9222..9818
                     /locus_tag="PKY13_05505"
     CDS             9222..9818
                     /locus_tag="PKY13_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT79420.1"
                     /translation="MGDPTTPQRSPSGRRRDPAIDERVLDATRASLVEHGWDGTSIRG
                     IAERAGVSRPAIARRWPTKAHLAIEALLGAEPDLGAFENVDLLGWVDGVVDGSFDLFA
                     RADMQSAMPALLATLRDHNDLRAAMWDGFAGPATDLVVAQGATATVTARAAIVMAAGA
                     ALFSSVVVGDDPALVDEVRVLVRAALAQSLAAAAATGS"
     gene            complement(9852..>10756)
                     /locus_tag="PKY13_05510"
     CDS             complement(9852..>10756)
                     /locus_tag="PKY13_05510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT79421.1"
                     /translation="GSGCRMGEQCSGRVALVTGASKGGTGTAIAVRLAAEGAAVALVA
                     RDAAGLDATAERIRAAGGSTITIPVDLADPDGGRATLVDRVADALGPVDILVNNAAAN
                     GYRPFTEWTSRQLEIAQQVNVWAPWRLMADVAPSMIERGEGWIVNLTSFAAELPPGPP
                     FPTNLVAQQGAGYGSTKAALNRLTVSVASELYPHGIAVNAVGPQSAIATPHLLARGDL
                     DPLLFEPLETMAEAVLLLATCDPATLTGRIGRSLELLAEVDRPVLGLDGNDLVEGWQP
                     TDLRGVVARLSERLAGLGWPDAFG"
     assembly_gap    10757..10856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10873..12084)
                     /locus_tag="PKY13_05515"
     CDS             complement(10873..12084)
                     /locus_tag="PKY13_05515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT79422.1"
                     /translation="MSKRVLYAGSVHDEAEIEACLDVLRSGPTAFRIGARVTAFEQQV
                     AALFGKAGGIAVNSGSSALYLAVELLDLPKGSEVLTSVLTFSTDVAPLVRAGLVPVFV
                     DVEPDTYNVDPDALERLIGPDTKAMLIPNLVGNTPDWDRLRAIADAHDLLVIEDSCDA
                     LGATLRGTPTGTRSDLSVTSFAASHIITAAGNGGMVLLDDEQLRDRGLLLRRWGRSSE
                     VQFYGSRKGDRRFLAEVDGMEYDSLFVFEELGWNFEPSELGAAFGLEQLKKLPNNREV
                     RQRNFASYHEFFSTFPEVFTPPVQTEGLDTAWLAFPFVIEPDCGIARSEFQRELEGRG
                     IDTRMVWTGNVTRQPMMRGVTYRSDPAGYPNADRIMEHALLLPCNHALTDEDVEFVCS
                     AVSDVVASHTS"
     gene            complement(12188..>13106)
                     /locus_tag="PKY13_05520"
     CDS             complement(12188..>13106)
                     /locus_tag="PKY13_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT79423.1"
                     /translation="LRTMAGMERADGGTIAVGDRVLTDAGSHVAPERRRIGLVPQEGA
                     LFPHLDVAGNVAFGLRRLASDERRRRVDELLELVDLAGHGGRRPHELSGGQQQRVALA
                     RALAPRPDVVLLDEPFSALDTALRSSLRDEVADVLAATGTTAILVTHDQEEALSLADH
                     VAIMRDGRIVQAGTPTDLYRRPVDRWTARFLGDANELPGRRTSPTSTEVGTPLGTVGL
                     DPLSPQPPGTELTVLVRPEQIVRDDGPGSVGAVVERVRFRGSDADVTLTVGPTTVTAR
                     WRSTELADAESAVMVRVTGPVIGYGVTGD"
BASE COUNT         1942 a         4334 c         4743 g         1887 t
ORIGIN      
        1 ccgccgtgat cgcgaccccg gtggcgacga atgcccagcc gtcacggtcg gcccgttcca
       61 tcgctgcgct ggcgaggagt gccaccgccg cgaccagcac cgtggcccac gtccagcccg
      121 tgccgcgttc gatctggttc cacaggagga agacggctcc ggcgacgatc gtgaccggcg
      181 cgatgcgccg ggcggccgca cgggcggcgt cgtggatctc cccctcggtc ttgagcgcca
      241 ggaagttggc gccgtggagg gcgaacagtg acgccgtgac gaccccgccg agcaacccga
      301 acgggttgag cagcgtgaag agcgtgccgg tgaagtcgcc ggccttgtcg atcggcacac
      361 cggcgacgat gttggcgaac gcgacgcccc acagcaccgc cggcagccac gagccgatgg
      421 cgatggcgag gtcccaccgc ttgcgccacc cgtcgtcgtc gaccttgccg cggtactcga
      481 aggcgacacc gcgggcgatc agcgcgacga ggatgaggaa gagcggcagg tagaacccgg
      541 agaacagcgt ggcgtaccag ttcgggaagg cggcgaacgt ggcgccgccg gcagtgagga
      601 gccagacctc gttgccgtcc caggtggggc cgatggtgtt gaccatgaca cggcggtcga
      661 cctcggtgcg gccgagcggg tgcagcagca tcccgacgcc gaagtcgaaa ccctcgagga
      721 agaagtagcc gacccacagg acggcgatga ggatgaacca gacggtggtg agttccatga
      781 cgtgcctcgt tctcgtgtcg gatcagtagg cgaaggccag cggctggccc tcggtgtcgt
      841 cggaaccctc ggcatcggga tcgggcatca cacccgccgg gccggccttg atgtagcggg
      901 cgaggagcct gagctcgacg acggcgagca gcccgtagat gagggtgaag cccacgagcg
      961 acgtgccgac ggtccacgcc gagaccttgg agacggcgtc ggcggtgagg agccgcacct
     1021 ccgggatgcc gttggggttc ggggcgacca cccacggctg gcggccgatc tcggtgaaga
     1081 tccagccgaa cacgttgccg aggaacggtg tggcaatcac ggcgatcgtg gtgaccggca
     1141 gccacttcca ccggggaagg tcgcggccac cccgggtgag gtagaggccg gcgacggcac
     1201 cgaaggcggc gatggcgccg aacaggatca tcaggcggaa accccagtag gtgatgaaca
     1261 ggctcggtcg gtagtcgccg gggccgtact tggcctggta ctcggtgttg aggtcgttga
     1321 cgcccttgat cgtggcgtcg gtcgtgtgcg tcgccaggag cgagagcccc ttggggatcg
     1381 cccaggtacg aaggttgcag tcggcgccct cctcaccgac aagggccagc accgacaacc
     1441 ccggaccctg ctcggtgttg cagagcgcct cggccgacgc catcttcatc ggctgctgtt
     1501 cgaacatgag ccgagcctgg aagtcgcccg agacggcaac gccgacaccg gcgacgaggg
     1561 tcagcgcgat cgctcctcgg agcgcccgtc ggagaccccg gcgatcgacc tcggcgagcg
     1621 tgtcgcgtcg cttcagcagc gaccaggcgg agatgccgcc gacgaaggtc gccgccgtca
     1681 tcagcgctcc ggcgatgacg tgggggtacg cggcgagggt ggtgctgttg gtcagcacct
     1741 tccagatgtc gaccatctcg gctcggccgg tgtcggggtt gaacttcacg ccgacggggt
     1801 gctgcatcca ggagttggcg gcgatgatga agtacgccga ggccgtggtg ccggccgcgg
     1861 cgagccagat cgtggcgagg tgcaccttct tcgggagacg gtcccagccg aagatccaca
     1921 ggccgatgaa ggtcgactcc aggaagaacg caacgagggc ctccatggcg aggggcgcgc
     1981 cgaacacgtc gccgacgaag cgggagtagt tcgaccaggc cattccgaac tggaactcct
     2041 ggacgatgcc ggtgacgacg cccatggcga agttcacgag gaacagcttc ccgaagaact
     2101 tggtgagccg gagataggcc tcgtcaccgg tgcggtacca acgggtctgg taccaggcaa
     2161 cgaccagggc gagcccgatc gtcagcggca ccatgaagaa gtggtacacg gtcgtgatgc
     2221 cgaactgcca tctcgcgaga tcgagtgtgt tcatgtcgtc ctctcatcgc tgtcagcgtt
     2281 gaccctacgg accactatct ctcatagata tggccaaagg tcctcatcgt gctgtgaccg
     2341 atgtcccgta ccgccggcgg cggtggccga tgatctgtga ccaagtgccc taggagcggc
     2401 ggtgggtccg ttcgtaccgt gtgggcgatc gtcgccgctc ggtggcgacg cgagcccgga
     2461 ggaaccaccc cgatgacctg ccagacgagc gtggcacgag gcccccgcac ctggtcgatc
     2521 gcagcgacga tcatggtcct cgctgcgtcg atgtggtcgg ccggctgtgc caacaccgcg
     2581 accggcgggg gtggagacgg agccggcgcc cggaagctca gcggtgtggt ggtcgagccc
     2641 gttcccgacg tgtcggccct gtcgctgccc gaggcgtcgc ccgccgccga accgttccgc
     2701 ttcgtcgcgg acggcggcga tctgctgctc gtctacttcg gctacacggc gtgtcccgac
     2761 gtgtgcccca ccaccctggc cgacctcaag gcggcgctga agcagctcgg tgcaccgtcg
     2821 aagcaggtgt cgatcgccat ggccacgatc gacccccggc gtgacaccgc cgacgtgatc
     2881 tcgggttacc tccacagctt cttcccctcg gcgaccgcgc tgcggaccga cgacgacgca
     2941 gcgctccgtt cggtcaccaa cgcattcgga gcgtcctacc tcaccgagta cccgaacacc
     3001 ggtgagccgc gggtgtccca cagcgcgtac ctctacgtcg tcgacgacca gggccacgtg
     3061 gtcctggcgt ggccgttcgg caccacctcc gccgacatcg ccctcgatct cgagcaactg
     3121 ctcgcctgac ccccgccggg cgccccgctc ggccgacaag gagaacacca tgaaagcccg
     3181 atccctcgcc ctcgcgtccg tcctgacgct cgctgtcctc ggtgcctccg cgtgcggcga
     3241 cgacaccaca tcggatgctg catcggacga gaccaccacg accctcgccg ccggcccgac
     3301 gatctcgggt gcgtgggcgc ggaactcgcc gatggaggcc accaacggcg ccgcctacct
     3361 ggcgatcacc ggtggaacct ccgacgacca gctcgtctcg gcgtcggtcg actcgacgat
     3421 cgccggcaag gtggaactgc acgagacggt ggccgccggt gattccggtg acgggtcgat
     3481 gggcgccacg acgatgggcg ccccgaccac tgctgcgggg gacaccatga cggccaccac
     3541 cctggctccg gccatggaga tgcgcccggt cgaggcggtc gaggtgccgg ccggcaagaa
     3601 ggtcgagctg aagcccggcg gctaccacat catgctgctc gacctcgttc agcccctcgt
     3661 cgtcggcgac tcgttcgacc tgacgctcac cttcgccgag gcgggcgagc aggtcgtgac
     3721 cgtcgaggtc aaggacgcac cgtgatccgg agggtcgcac ggcgagcggc gttcggcatc
     3781 gcgctggtgg cgttcgtcgt gtcgtcgtgc ggctcgcccg tccgggcgtc gaagcagtac
     3841 cactacgtca tccccgaagg aacggccggc ctcatcgcca cgaaccgcgc acccgagatc
     3901 ttcccggccg acctgaccgt ccgagtcggt gactcgatca cgatcgtgaa cgaggacgac
     3961 ttcgaccagc aggtcgggcc ctacctcgtg aaggcgggga ccacgatgac ccagcacttc
     4021 tcgtctccgg gcgtcttgga gggcgcctgc acgatgagca tgggtggcac gctgagcgtg
     4081 acggtgctgc cggcggagtg acctcagggg ccggtcgtgg tggtcgccgg gaccccgagt
     4141 tcgtcggcga tcagccgttc gagttcggcc tcgtcgagcg ccccgttgtg ggtggcggcg
     4201 atggtgccgt cggcgcggat gaacgccgtc gccggcatcg tggtgatgga cagcgcgacc
     4261 tgtgaggtgg agtcggggtc gaggtactgg tcgaacccga cgccggtctc gtcgacgaac
     4321 tgctgcgcgg ccacctcggt gtcgccgagg ttgacgccga cgaagcggac gaccccctcg
     4381 tacttcgagg cggtgacgtc gaaggccggc atctccttgc ggcacggggc gcaccacgtc
     4441 gcccagacgt tcaggaccat cggcgttttc gaggcgctgc cgagttggag tgggggagcg
     4501 tcggcgagcg gcgtgaccgt gaccgccgcc agggtgtcgt catcgcccga cgacgacgtc
     4561 gtgtcgtcgg aggagttgca gcccaccgcg gtcaggccga gcagtgccgc aacgagcagt
     4621 gcgacgacgg aggtcggggc tggccggggg tggcggtggc ggttcgttct cgtgtccacg
     4681 tctcgtcacc tcgcgtcggg cccggtcgtc gggatgaggt cacgctagag cggtgaaccc
     4741 gcggccgtca cacccggccc gggggttctg atcgatcggg agcaggcgcg aacgcgttga
     4801 ctcgcggctg atctccaaat accctggggg gtatgtgcag agcaacgaca tgttcgaagt
     4861 gccacaagcc cacctggtcc ggttgcggtg cccacgtcga gcaggtgctc gnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagtgccac aagcccacct ggtccggttg
     5041 cggtgcccac gtcgagcagg tgctcggcga cgtgccgccg gcccagcgct gccactgtcg
     5101 tgaggacaag gcagcagcga aggcgacgtc cgcggcgtcc gatgccggcc gcggtgggtt
     5161 gttggcccgc ctccggggca agtaggcact ggaggaacac tgacgacgaa cggagctccg
     5221 atgacgaaga cggtgatcga atgcccggcg tgtggggtgc ggaaccgggt ggccgacgac
     5281 gccggcggcc gcccgcgttg cagcgcgtgc cacaccgacc tgccctggct ggtcgacgtc
     5341 ggctcggccg acttcgacgg ggtcatcgaa cgttccgcgc tgcccgtcct cgtggatctc
     5401 tgggcgccgt ggtgcggtcc gtgccgggcg gtcgcgccgg cgctcgaaca gctggccatg
     5461 gatcgtgccg gcgccctgcg gatcgtcaag gtcaacgtgg accaggcgcc ggaggtctcg
     5521 gctcgcctcg gcgttcaggg gatcccgacg atggtgctgt tcttcgacgg agccgaactc
     5581 tcccggcagg tcggtgccgt gcccggcccg gcgatcgccc ggtgggtcga cggcgcgctc
     5641 ctcggcgtca agcgggcctg agcaacgtcg gcctcaggcg gccacgccgt cgagacgctg
     5701 gcgcagccct gcggcggcac ggtcgccgat gcgtcggaac aacaacgcca gcaacgggtc
     5761 gacgacgcgg agcgggccgc gcagccggag gtcggcgtcg taggtcacga tgcatcccga
     5821 gtcgccgtcg ggtacgaccg tgatcacgtc gaccgactcg agcgtcgacg tctgcgaccg
     5881 tgcctcgacc gaggtgccgg gaacggcgtc gacgatctcg tagcgcatga ccgtgccgcc
     5941 gccgatgccc ttcaccgtga ggtcgtaggt cgagcccacc gcgccggctt cgccgctgac
     6001 gagcaccgcg gcggtgatgc cggggtccca ctcgtcgaaa cggctcaggt ccttcatgac
     6061 gtcgtaggcg tcggcgggaa gccacgggga gcggacgctc gtgcgatatc gggccatcgg
     6121 gactcctcgg gttcggatgc ggcgaccgat cggctcgacg tcgacgcccg caacgatcct
     6181 cgcaggctcg ccgtcgatcc ggtcgtcagc cggtcgtcag ccggtcggca ccgggatcaa
     6241 cgcgatcggg cagcgcgccc cgtggagcgt gcggaacgcg gtcccgccgg cgcggacccc
     6301 gagggggccg agcggaccga gcccgatcac gagcaggtcg gcgccatgag cggccttgag
     6361 gatgccgtcg gtcggccgct cgccgcgacg ggccacggtc gtcaggtcga tcccggcgtc
     6421 gacgagctcc ggcgccaagt cgtcgcggat ccgtcgttcg gcgtcacgac gccacccctc
     6481 gggatcgggc ttgggcgacc actcgaagaa gggctcgtgc acgttcaccg cggtcacggc
     6541 cgcgcccgtg gcagcggcga ggtcggcgca ccaggcgacg gccgcccggc tggccggcga
     6601 gccgtcgaca ccgacgacga tgcgcacgaa cgtcacgtcg gcgtcgtcgg ggacgaccac
     6661 cagggggacg gtcgtgtggt gggcgaggta ctcggcgacc gaaccgacat ggaacaggcc
     6721 cggtccgctg ccgtgaccgg cacgaccggt gacgatcaga gtcgcgccgt gggtgtgggg
     6781 cgacccggtg agcacctcac gggggtcgcc gccggcgaca tcggtgaccg cggtggtcac
     6841 cggcgccgag ccgggtccgt cggcgggatc caggcgcctc cgctcagccc gcatcgcctc
     6901 gtcgaggcga acgtggtcac cggtgcccgg catcgacgcg ccgagcagga gtacgtcggc
     6961 gccggtgtgc tggccgacag cggccgccca cgccgcggcg cggcgtgatc cctcggaatc
     7021 gtcgaacccc accacgaccc gattgctacc tgtggtcacc atcgtcgctc ctgtcggtac
     7081 ggctcacgat ctcatcgtcg tgcgaccgag gcctgggcga cagggcccaa cgtcccgagg
     7141 cgagcaccgg cacagcaccc gctacaaccg ggcggtcagc cgtccgatga ggccggcccg
     7201 gccgggggcg acgcgcgacc acaacgagac gaggtgggcg tcggggccca cgacggcgcg
     7261 gccccggtcc tgttcgatcg cggcgacgat gcgcttcgcc acggcggacg gtggccgcat
     7321 ggcgagcggc gcgagtcgtg atcggccgag ctccgccaag cggcccgact ggctcccacg
     7381 cgccgagttc gtgatgttcg tgttgatggc cccgggaaac accgccgtga cgccgatgtt
     7441 ggtcgtgacc agctcgttgc ggagcgcctc gctgaagccg cgcactgccc ccttggtgag
     7501 cgagtacgac gcgttctggg gcaggccgag gagtccgacc atgctcgaca gattgacgat
     7561 gtgggcctcg tcggcctcac gcaggaccgg gaggaacgcc cggcagccgt tcacgacacc
     7621 ccacacgttg atgtcgacga tccagtgcag gtcgtcgagc gactcgtcct cgaacgggcc
     7681 ggccgaggtg accccggcgt tgttgacgag gatgtggcag gcgccgtggg cgtcgacgac
     7741 agcagcgggg agcgcggcga gcgcgtcggc gtcacgcacg tccacgatgt gggtcgacgc
     7801 gtggcgaccg agggagcgga tcgtcgtcgc cgtctcctcg acggcggtcg gtgagatgtc
     7861 gacgagcgca aggtggcagc cggcccgggc cagggcgtcg ctggtggcgc ggccgatgcc
     7921 gctgccggcc ccggtgacga cggcgacgcg gtcggcgagt cgcttcatgc gttgacctcc
     7981 ggggtggtga gcacgcaccg ggtgcccgtg aagatcgtgg gcatgcactt gttgcagtgc
     8041 acgcagaggc tggccgattg ctctccgtcg cgcatgcggt tcggcaggtc gggttgacgc
     8101 aggagcgccc gcgccatcgc cacgaagtcg aagccctcgg cgagcgcgcc ctcgatcgtc
     8161 gccagctcgt tgatgccgcc gaggaggatc aacggcatcg acagcgcggc gcggaactgg
     8221 cgggcgaagg ggaggaagaa cccctcctcg aacggcatct cggggaagat cttgtggccc
     8281 atgacgcgga ggccgagccc gaccgccgcg ggctgggtgg cgatgaactc cgccatcggc
     8341 acgtcgccgc ggaagaagta catgccgttc aagagggagc tgccgccggt gagttggagg
     8401 gcgtcgagct gcccgtcggc ctcgagcatg gccgcgaacg gcaggctctc gtcgagccac
     8461 agaccctggg gcacgccgtc ggccatgttg aacttggcgg tcacggcgac gtcggtgccg
     8521 acggcggcgc gcactgctgc gatggcgcgg cgggggagcc gcatgcggtt ctcgaggctg
     8581 ccgccgtact cgtcgcttct cttgttgagg ttggcgctga ggaacgagct caggaggtag
     8641 ttgtggccga ggtgtacctc gatcgcgtcg aacccgctct cgacggcgac gcgtgcggct
     8701 cgcccgaact ggtcgacgac ttcgtcgagt tcggcgtggg tggcgccgcg gacgagcccc
     8761 atggcaggcg ggctcaagcg ggttgacggg gccagtgtct tggtgcggtt cgagcgggtg
     8821 ttggcgacga ggccggcgtg tccgatctgg gccgcggcaa gtgctccctc ggagtgcacg
     8881 gcgtcggtca gtcgacggag gcggggcacg atctgatcgg acatcaccat ggtgttgcgc
     8941 tggacccgac ccccagggct gacggcgcag tacgcaacgg tggtcatcgc ggccccgccg
     9001 gcggcgacgc gacggtggaa ctcgacgagt tcgtcgctca ccgcgccgtc gggcatcacc
     9061 ccttcgaacg tggcggcctt gatgatgcgg ttgcgaagcg tgagcggccc gagccgggcc
     9121 ggctcgaacg ccgtggacgt gctggactcg gtcatgtgtc gtccctcccg acccgaacga
     9181 taccactggt atcggaatgt ggctggcaga ctgtacggcc tatgggcgac cccaccacgc
     9241 cgcaacgttc gccgagcggt cgtcggcgcg accccgcgat cgacgagcgg gtgctcgacg
     9301 cgacccgggc gagcctcgtc gagcacggct gggacggcac gagcatccgg gggatcgccg
     9361 agcgggcggg tgtgagtcga ccggcgatcg cccgacgatg gccgacgaag gcccatctcg
     9421 cgatcgaggc gctcctgggc gccgagcccg acctcggagc cttcgagaac gtcgacctcc
     9481 tcggctgggt cgacggtgtg gtcgacggca gcttcgacct gttcgcccgg gcggacatgc
     9541 agtcggcgat gcccgcgctc ctcgccacgc tccgcgacca caacgacctc cgcgccgcga
     9601 tgtgggacgg cttcgccggt ccggcgaccg atctcgtggt cgcgcagggc gccacggcga
     9661 cggtgacggc tcgcgccgcg atcgtcatgg cggcgggcgc tgcgctgttc agcagcgtcg
     9721 tcgtcggcga cgacccggcg ctggtcgacg aggtgcgggt cctggtgcgg gcggcgttgg
     9781 cccagtccct cgccgccgcg gcggccaccg ggagctgagc tcgatcgcgt ccggcgtcgg
     9841 cgtaaccgga gtcagccgaa ggcgtctggc cagccgaggc cggcgaggcg ctcgctcaga
     9901 cgtgcgacga cgccccgcag gtcggtcggc tgccacccct cgacgagatc gttcccgtcg
     9961 aggccgagca cggggcggtc cacttcggcg aggagttcga ggctccggcc gatccgtccc
    10021 gtgagcgtgg ccgggtcaca cgtggcgagc agcaggacgg cctcggccat cgtctccagg
    10081 ggctcgaaca gcagggggtc gaggtcaccg cgggcaagca gatggggcgt ggcgatggcg
    10141 gactgcgggc cgacggcgtt cactgcgatc ccgtgggggt agagctccga cgcgaccgac
    10201 accgtcaacc ggttgagcgc ggccttcgtc gagccgtagc cggccccctg ctgggcgacg
    10261 aggttcgtgg gaaacggcgg accgggcggg agttcggcgg cgaaactcgt gaggttgacg
    10321 atccagccct cgccacgctc gatcatcgac ggtgcgacgt cggccatcag gcgccacggc
    10381 gcccacacgt tgacctgctg ggcgatctcg agctggcggc tcgtccactc ggtgaacggt
    10441 cggtagccgt tggcggcggc gttgttgacg aggatgtcga caggcccgag cgcgtcggcg
    10501 acccggtcga cgagggtcgc tcggccgccg tcgggatcgg cgaggtcgac ggggatcgtg
    10561 atcgtgctgc cgccggcggc gcggatgcgt tccgcagtgg catcgagccc ggccgcgtca
    10621 cgggccacga gcgccaccgc agcgccttcg gctgcgagcc gcacggcgat ggccgtgccc
    10681 gttccaccct tgctggctcc ggtcaccagg gcgacgcgcc cgctgcactg ctcacccatg
    10741 cggcaccccg atccgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaccc
    10861 cgatccgatc gatcacgacg tgtgcgacgc caccacgtcc gacacggcgc tgcagacgaa
    10921 ctccacgtcc tcgtcggtca gggcgtggtt gcaggggagc aggagtgcgt gctccatgat
    10981 gcggtcggcg ttggggtagc cggccgggtc gctgcggtag gtgacgccgc gcatcatcgg
    11041 ctgccgggtc acgttgcccg tccacaccat gcgcgtgtcg atgccgcgac cttcgagttc
    11101 acgctggaac tcggaccggg cgatgccgca gtcgggttcg atcacgaacg ggaacgccag
    11161 ccacgcggtg tcgaggcctt cggtctgcac cggtggggtg aagacctcgg ggaacgtgga
    11221 gaagaactcg tggtagctcg cgaaattgcg ctggcgcacc tcccggttgt tcgggagctt
    11281 cttgagctgc tcgaggccga acgccgcgcc cagctcggac ggttcgaagt tccagccgag
    11341 ctcctcgaac acgaagaggc tgtcgtactc catgccgtcg acctcggcga ggaagcgccg
    11401 gtcgcccttg cgcgaaccgt agaactgcac ctcgctgctg cggccccagc ggcgcagcag
    11461 caggccgcgg tcgcgcagtt gctcgtcgtc gagcaggacc atgccgccgt tgccggccgc
    11521 ggtgatgatg tgcgatgcgg cgaagctggt gacgctgagg tcggaacggg tgcccgtggg
    11581 cgtgccgcgg agcgtggcgc ccagcgcgtc gcaggagtcc tcgatgacga ggaggtcgtg
    11641 ggcgtcggcg atcgcgcgaa ggcggtccca gtccggggtg ttgcccacca ggttggggat
    11701 gagcatcgcc ttggtgtccg ggccgatgag gcgctccagc gcatcggggt cgacgttgta
    11761 ggtgtcgggt tcgacgtcga cgaacaccgg gaccaggccg gcccggacga gcggggccac
    11821 gtcggtcgag aacgtcagga ccgaggtgag gacctccgat cccttcggga ggtcgagcaa
    11881 ctcgacggcg aggtacagcg ccgacgaacc cgagttcacg gcgatcccgc cggccttgcc
    11941 gaacagcgct gcgacctgct gctcgaacgc cgtgacccgc gcaccgatgc ggaacgccgt
    12001 cggcccggat cggagcacgt cgaggcacgc ctcgatctcg gcctcgtcgt gaacacttcc
    12061 ggcgtagagc acccgcttcg acatcgtgga tccagtcagt tcaggccgaa caggtgtgat
    12121 cgatcgtgac accacgtccc ccggtcgtgc cgcatcctat ggacggggac cccctcggtc
    12181 gaccgcctca gtcgccggtc accccgtagc cgatcaccgg cccggtgacc cgcaccatga
    12241 cggcgctctc ggcgtcggcc agctcggtcg accgccatcg ggcggtcacc gtcgtcggtc
    12301 ccacggtcag cgtcacgtcg gcgtcggagc cgcggaagcg gacccgttcg acgacggcgc
    12361 cgacggagcc cggtccgtcg tcgcggacga tctgctcggg gcggacgagc accgtgagct
    12421 cggtgccggg cggttgcggc gacagtgggt cgagccccac ggttccgagc ggggtgccga
    12481 cctccgtcga tgtgggcgac gttcgtcgcc ccggcagctc gttcgcgtcg ccgaggaacc
    12541 gggccgtcca ccgatcgacc gggcggcggt agaggtcggt cggcgtgccg gcctggacga
    12601 tccggccgtc gcgcatgatg gcgacatgat cggcgagcga cagggcctcc tcctggtcgt
    12661 gggtcacgag gatggcggtc gtgcccgtgg ccgccaggac gtcggcgacc tcgtcgcgga
    12721 gtgatgagcg cagcgccgtg tcgagcgcgc tgaacggctc gtcgaggagg acgacgtcgg
    12781 gtcggggcgc gagcgcacgg gcgagcgcga cccgttgctg ctgcccgccc gacagttcgt
    12841 gggggcgccg gccgccgtgg ccggcgagat cgacgagctc gagcagctcg tcgacgcggc
    12901 ggcgccgctc gtcggacgcc agccgacgga gcccgaacgc cacgttcccg gcgacgtcga
    12961 ggtgcgggaa caacgcgccc tcctggggga cgagaccgat gcggcgccgt tccggcgcga
    13021 cgtgagagcc ggcgtcggtg aggacccggt cgccgacggc gatcgtgccg ccatcggcgc
    13081 gttccatgcc ggccatggtt cggaga
//
LOCUS       DAOSOA010000056        13081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4775_length_13081_cov_7.111700, whole genome shotgun sequence.
ACCESSION   DAOSOA010000056 DAOSOA010000000
VERSION     DAOSOA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13081
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4775_length_13081_cov_7.111700"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PKY13_05525"
     CDS             <1..979
                     /locus_tag="PKY13_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT79424.1"
                     /translation="HDTLRFWLDRGVDGFRMDVVHLIGKDPVLPDEPPDVAGLPHTIL
                     NDRPETHALLRELRTLLDSYDGDRMAVGEVYLLDTDLVASHYGNGDELHLCFNFPPLY
                     APFDADAWRTQISTTTAALGPRDAWPTWVLSNHDNPRHRTRYGNSEEVARAAAVLLLT
                     LRGTPFLYAGEEVGMQDAVVAPEQVHDPAGRDGCRAPLPWTPEAPHGWATDDTWLPWP
                     PDAAAANAATQQDDPASMLSLYRTLLSLRRSTPALSLGDLALHLDTGPVLVFDRTDGP
                     STFTVAINMSGADTTTTAAGTLVLSSRAGGRSTFDGTLDAYEAVVLSAG"
     gene            complement(966..1934)
                     /locus_tag="PKY13_05530"
     CDS             complement(966..1934)
                     /locus_tag="PKY13_05530"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOT79425.1"
                     /translation="MLAIEASDLLANAGRLGILAVPALLGVLVVRLRQVPDVPAAIGA
                     LNTYTLHLGFPALIAVGVLEVDPSVASTWGFWAVIPVVDLVLIAISWAVGRRLAGRQG
                     GSIAIVLLFGNTAYLGLPFAVSVLGEPARGPASLLVAVQVTLAVLVGPVLLQRWSGDG
                     HGSVDWARLFRQPLLLAPFAAVMVRQLPDGVVGGVHDVLGPLAASTAPVAMFLLGLYL
                     VENWELVRTAEHGVWTHVLLRMVVAPAVNAAVAVWLFEIGTISSTTMAVIVVLGGMPA
                     AISTFSIAHHEGVAADRVASVVVRSSVTALVTMPVLATVAEQLSRR"
     gene            2015..2389
                     /locus_tag="PKY13_05535"
     CDS             2015..2389
                     /locus_tag="PKY13_05535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT79426.1"
                     /translation="MSDTKPTVTIPDTEPPAELVIDDLVVGDGAEATKGANVIVHYVG
                     VAWSNGQQFDASWDRNDPFEFRLGAGQVISGWDEGVAGMKIGGRRQLTIPPHLGYGAQ
                     GAGGVIKGGETLVFVVDLLNLG"
     gene            2477..3658
                     /locus_tag="PKY13_05540"
     CDS             2477..3658
                     /locus_tag="PKY13_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79427.1"
                     /translation="MGHTSATNGGPSRLDGHAPPVSGPIGGALTPLCLRSLVDSYDHG
                     RQLFSHQLFDGVWREVDDLYPHETLTSSSIALLGLARSGESGDVHGIDVQRCVDAIGG
                     EVTRTGYRAGIGLALWANAAVDGAADAAGYLAPLGLSIDSLVEQHVAGFTTMETAWLA
                     SGLLHEFARTGDTAVRAAAHAVVDELRTNRYVGATRLMRHAGPGASALHRARSHIANF
                     ADQIYSVQAFAFAAIVLDDAAARGVGSALAAHHVELQGDRGQWWWHYDAQRGHVALRY
                     AVYSVHQHGMAPMALAAMARADGGDYSAAIAASRRWITDNELGSSMVDVERSTVWRSV
                     ERVEPTVITNVRGGLEALGVADHRAAEVTLNRETRPYEWAWCLYAAAIENDLPRGTSL
                     V"
     gene            3651..4451
                     /locus_tag="PKY13_05545"
     CDS             3651..4451
                     /locus_tag="PKY13_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOT79428.1"
                     /translation="MSDPAAEVPAAALPSAAPVDVFDWQLAALRMPEVVADLRRPADH
                     TRLVMTVNVALMWMAARDDRLGHIVQDAAIRVADGMGVIWTWRALGRRLPERIAGIDL
                     MVQLIDAAAADGRSIYLLGAQQHVIDQLRHVLLQRNPSLIIAGSRNGYFTDQEHHDVV
                     SDVKRSGADLLFIGMPSPFKEVFAADHLDEFGADLVMGVGGSFDVISGFVPRAPSMLQ
                     RTGFEWAWRLACEPRRMWKRYAVTNAWVARQLVTRMVRRRRRRPDPSS"
     gene            complement(4473..5078)
                     /locus_tag="PKY13_05550"
     CDS             complement(4473..5078)
                     /locus_tag="PKY13_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79429.1"
                     /translation="MKLLRQTAAAVSVSALAVLGLQMSAGAAVPSISASDTTIPAGGS
                     VNVSLGEPCLDSEGEPTYGDVISSASSGDTHQETKGAGFIKGATGTFVFDTPGTYTIS
                     RFCDGQGLTSQVTITVTAPTTTTTTSTTTTAAPTTSTTTTAPKSTTTTTAPKSTTTTA
                     KVADATATTTTTTAAVKVESVTSTKATGATRVQAELVSYTG"
     gene            complement(5235..6482)
                     /locus_tag="PKY13_05555"
     CDS             complement(5235..6482)
                     /locus_tag="PKY13_05555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT79430.1"
                     /translation="MKVAYLTGEYPRATDTFIQREILAVERCGVDVERFAVRRPGDEH
                     MVGERQRSERDLTTYLLEQPSSSYAGVLARALRRSPRRFLAALRLAVRTRRLGPRGAA
                     KQAAYFVEAAVLAELMAERDVDHLHNHFADSSCTVAMLASELSGTTYSFTLHGPAIFF
                     EPNEWHLGTKLETAAFCATISNFARSQACLFSPSEVWPRIRIVHCGVDEADVADATAS
                     DGRCQLLFVGRLDAVKGVVVLFDAVASLVAAGNDLHLTLVGDGPQRSELERRSVELGI
                     ASSVTFAGYRNQEQVQQHLRESDVFVLPSFAEGVPVSLMEAMAMTLPVVATNVGGVTE
                     LVRDGRNGKVVAPGDAVALEAAIAELAADPDLRRRLGEVGRATVRADFDSLTEARRLV
                     TLFSDPASPSPGMVRPTPAPLAD"
     gene            complement(6479..7492)
                     /locus_tag="PKY13_05560"
     CDS             complement(6479..7492)
                     /locus_tag="PKY13_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT79431.1"
                     /translation="MRVLVTGSTGFVGAYTVAEALHAGHDVTALVRPASKAPFAALAD
                     HPRLTLARADLRDRRSLDGLFDEIDVVIHLAAAKSGDFATQFAGTVLSTENLLAAIDG
                     SPVTRLVAVSTFSVYDYSHLKAGSVIDETTPIDLHPTRRDEYAQTKLLQEQLYRAEME
                     RREVVILRPGMIYGRDNLWHALLGAELGPLFLRIGSRATLPMTYVENCVAAMVAAIDA
                     PGVASETINIVDDDLPTQAEYVAALERQTEIPSSIPVPWPLMKLAATTISAVNNRFLD
                     GRAKFPGIVVPEKLDGRFKPFRYNNDRARRLLGWAPQYTLAQAFSRSLSDTDLVVERA
                     GSA"
     gene            complement(7489..8604)
                     /locus_tag="PKY13_05565"
     CDS             complement(7489..8604)
                     /locus_tag="PKY13_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT79432.1"
                     /translation="MVEPESGTGPRLRAAVIGTGGISKEHLRFLAGSPRADLVGVCDM
                     SPIAVSHAATTYGTTAYATVDEMLAGARPEVVHVLTPPTTHVALSELCLAAGADVICE
                     KPITLGAADLERLLAFADDAGRRICENHNYRFNPEVLELDSLVESGVLGRVGEVEVRM
                     GLPVNDPDGRFGDPNLPSPIHQLPAGVIHDFITHLTYLVDNFTPGVEWLTVDSRWSNH
                     GGVAHVAVDDLDSILVGDGPDGAVHARIRFSALLAPDCFEITVRGTEGYAQVELFSGR
                     VSRVIPRPGGKQLSPIVNMMVGGATMFAGGPRSLKRKIMQQGPYVGLERFLALTYTAL
                     AAGTELPVTPADMLATSRLIDRLVAGRSVGAAPEVVS"
     gene            8787..9617
                     /locus_tag="PKY13_05570"
     CDS             8787..9617
                     /locus_tag="PKY13_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOT79433.1"
                     /translation="MKRATSHVDGLTRVELFGLDFVDDTSIARVADLVLDSTPDDVEG
                     HPVIVTPNTDLLLQVTKTPDARIREFFAKAWCVLPDGQPIVWASRILGSRLRFRLTGS
                     DLFADLWPRLTARATPVFVLAPSAAVARGLEESNPAAATLVAPMLDVGSRSDLVDLAA
                     QCVAEITAVAAKVVILSLGHPKDVMIADEILRQLDASPTALPFILCLGGSAEMFLGIR
                     KRAPLWVQKCSAEWLYRFAQEPRRLFHRYFVRDIGFIPIVWREYQAKRRNDAAAGHRA
                     "
     gene            9641..10486
                     /locus_tag="PKY13_05575"
     CDS             9641..10486
                     /locus_tag="PKY13_05575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT79434.1"
                     /translation="MSVVMPIGGIDEFLPQQLDALAEQEFDQPWELVLSMNQPGTTHD
                     PAVAAPRLHPLATSLIVDSSDERSAAHARNVGARAASADLLAFCDADDIVEPTWLRAL
                     VESLGSHAAVGGYLDERRLSPPGQERWRPPATPDELPSYLGHRYPVSANMATTREAFD
                     AVDGFVEGLTRCEDIAFGWALADAGIDLAYAPDAVVHYRHRPGLWTMVRQHHYYGIGM
                     TEVIERHGLPGGAQPSGLLQANKQRVTQRSVIHILRRGAIASGRLRGLAQERLTRRHR
                     TQAAR"
     gene            10537..12138
                     /locus_tag="PKY13_05580"
     CDS             10537..12138
                     /locus_tag="PKY13_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79435.1"
                     /translation="MEWSFIVAAVRRRIRFVVLCAILGAVPGLLSIANSVSSYEAVAT
                     IGIATPTSGAGAAVAASQPDRYITSQLSVVTSTDTLDKAAKKLGGGVTAKQIAEQLTV
                     EQEPSADIITVKATASRPKAAAAIANAIADVYITEAASREAAARAPRIQTLQDKLDAI
                     TTQLTTPDTGIYAQITAAMKDLIAKANEAGSTGYVPSESLVVPGLVQQRDALNAEYNR
                     VLQAKNELELSVDDQSVSSLIERATAPSSPTQTKGKIILAGGTVLGGLIGVAVALVLA
                     QLSSKVLDEVSVAQMLGTTVVGAFPRNRALLDNPLAALKTTPADARETMERLSVRAEA
                     MADPEIDHALTIAVVGTQPAAGTTTLTLALARRLSRSGYSVVIVDGDLRTQTVSEIFD
                     TDPSTGISAVLAAEVKKPTKPVNYFTDTDLPRVEVLGVGKIERREALRRDAIGPILEV
                     ARERGQIVLYDGGSLMGSALTLFASKAADVIVLAVPLLEQDQEMLSEVADNLPTERSR
                     VLAVTTAPVRRRHRRPDTAEAEPKD"
     gene            12141..>13081
                     /locus_tag="PKY13_05585"
     CDS             12141..>13081
                     /locus_tag="PKY13_05585"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT79436.1"
                     /translation="MNRTLGYRPGLDGVRAVAIGLVLLNHTGLSIFLGGGNGVIVFFV
                     LSGFLITKLMLEEWDRSGTISVGGFYGRRAVRILPAPTVMLVVLLAASWAITETPSEH
                     SYLLKEILLAATYLTNMKPLLLNDVFVNGAVYDGFLGHMWSLAVEEQFYLVWPLTMIA
                     LALPSRNARRSVTAIVGVAVAIAVVRLGLYYLPGLDPDLASISLFSFDGFALGAAMAF
                     AFHRGTFPAFERLLSRPAVPVLAIAVLVGDLLAGRATHEISSLYVVYTSVAAAALIGH
                     LFVAPDSFASRWASLPPVVLIGKLSYSIYLWHNPIWT"
BASE COUNT         2039 a         4661 c         4393 g         1988 t
ORIGIN      
        1 gcacgacacg ctgcgcttct ggctcgatcg cggcgtcgac ggcttccgca tggacgtggt
       61 ccacctgatc ggcaaggacc cggtgctccc cgacgaaccg cccgatgtgg ccggtctccc
      121 ccatacgatc ctcaacgacc ggcccgaaac ccacgcgctg ctccgcgagc tgcggacgct
      181 gctcgactcc tacgacggcg accggatggc cgtcggcgag gtctacctgc tcgacaccga
      241 cctggtggcc tcccactacg gcaacgggga cgagctgcac ctgtgcttca acttcccgcc
      301 gctgtacgcc cccttcgacg ccgacgcgtg gcgcacccag atctccacga cgaccgcagc
      361 gctcggacct cgcgacgcat ggccgacctg ggtgctgtcg aaccacgaca atcctcgaca
      421 ccgcacccgc tacggcaact ccgaggaggt ggcgcgggcc gcggcggtcc tgctcctcac
      481 ccttcgaggc acaccgttcc tgtacgccgg cgaggaggtc ggcatgcagg acgcggtcgt
      541 cgcgcccgaa caggtgcacg acccggccgg tcgtgacggg tgccgggccc cgttgccgtg
      601 gacgcccgag gctccccacg gctgggcgac cgacgacacc tggctcccgt ggccacccga
      661 cgccgctgca gcgaacgccg ccacccaaca ggacgacccg gcgtcgatgc tgtcgctcta
      721 ccggacgctg ctctcgttgc gacgctcgac gccggcgctg tcgctgggcg acctcgcgct
      781 ccacctcgac accggcccgg tgctggtgtt cgaccgcacc gacgggccct cgacgttcac
      841 ggtcgcgatc aacatgagtg gggccgacac gacgacgacg gccgccggga cgctcgtgct
      901 ctcgagccga gcgggtggcc ggtcgacctt cgacgggacc ctcgacgcct acgaggccgt
      961 cgtcctcagc gccggctgag ctgttcggcg accgtggcga gcaccggcat cgtcaccagc
     1021 gccgtgacgc tcgaccggac gacgacgctg gcgacgcggt cggccgccac gccttcgtgg
     1081 tgggcgatgg agaacgtact gatcgccgcg ggcatgccgc cgagcaccac gatcacagcc
     1141 atcgtcgtgg acgagatggt gccgatctcg aacagccaca ccgccaccgc ggcgttgacc
     1201 gcaggggcga cgaccattcg gaggagcacg tgggtccaca caccgtgctc ggcggtgcga
     1261 acgagctccc agttctcgac caggtagagg ccgaggagga acatcgcgac cggcgccgtg
     1321 gacgcggcga gcggaccgag cacgtcatgg acgcctccga cgacgccgtc gggcagctgt
     1381 cgaaccatga ccgcggcgaa cggcgcgagc agcagcggct gacgaaacaa ccgcgcccag
     1441 tcgaccgacc cgtggccgtc accgctccac cgttggagca gtaccggccc gaccagcacc
     1501 gccagcgtga cctgcacggc gacgaggagg gacgccgggc cacgggccgg ttcaccgagc
     1561 acgctcaccg cgaacggcag tccgaggtac gccgtgttgc cgaagaggag cacgatcgca
     1621 atcgatccgc cctgccggcc ggcgaggcgt cgaccgacgg cccagctgat cgcgatcagc
     1681 accagatcca cgacggggat gactgcccag aagccccagg tcgacgcgac cgaagggtcc
     1741 acctccaaga cacccaccgc gatcagcgcc gggaagccga ggtgcagggt gtaggtgttg
     1801 agcgctccga tcgcagcggg aacgtcgggc acctgacgca gccgcaccac caggacgccc
     1861 aagagcgccg gcacggccag gatgccgaga cgaccggcgt tcgccaacag gtcggacgcc
     1921 tcgatcgcca acatcaccgg caccatcatg gtcgttgcgg cgcccggccc gggtcgtgat
     1981 cgcgccggtc cggtcgcggt ctatggtcac gcccatgagt gacaccaagc cgaccgtgac
     2041 gatccccgac accgagccgc ccgccgagct cgtgatcgac gacctggtgg tcggcgacgg
     2101 cgccgaagcc accaagggtg ccaacgtcat cgtccactac gtcggggtcg cctggtcgaa
     2161 cggccagcag ttcgacgcca gctgggaccg caacgatccg ttcgagttcc gcctcggagc
     2221 gggtcaggtg atctcgggct gggacgaggg cgtcgccggc atgaagatcg gtggtcgccg
     2281 ccagctcacg atcccgcccc acctcgggta cggcgctcag ggcgctggcg gggtgatcaa
     2341 gggcggcgag accctcgtct tcgtcgtcga cctgctcaac ctcggctgat cgcggccggt
     2401 cctccggccc cctcgatccg ggacaatcgg gtgccatccg tcccgaattc caatatctgt
     2461 ggctatcgtg agcgccatgg ggcacacatc tgcgaccaac ggcggaccgt cgcgcctcga
     2521 tggccacgcg ccgccggtgt cgggtccgat cggcggcgcc ctcactccgc tctgtctgcg
     2581 cagcctcgtc gactcgtacg accacggccg acagctcttc tcccaccagc tcttcgacgg
     2641 cgtgtggcgt gaggtcgacg atctctaccc ccacgaaacg ctgacgagca gcagcatcgc
     2701 gctgctcgga ctcgcccgct ccggcgagtc gggcgacgtc cacggcatcg atgtgcagcg
     2761 gtgtgtcgac gccatcggcg gtgaggtcac ccgcaccggc taccgggcgg gcatcggcct
     2821 cgcgctgtgg gccaacgccg ccgtcgacgg tgcggcggac gccgccggct acctggcccc
     2881 gctcgggctc tcgatcgatt cgctcgtcga acagcacgtc gcggggttca cgaccatgga
     2941 gaccgcgtgg ctcgcgagcg gtctgctcca cgagttcgcg cgcaccggcg acacggcagt
     3001 tcgcgccgcg gcgcacgccg tcgtcgacga gctccgcacc aaccgctacg tcggagcgac
     3061 tcgactgatg cgtcacgccg gtccgggcgc ctcagcgctg catcgggccc ggtcccacat
     3121 cgccaacttc gccgaccaga tctactcggt ccaggcgttc gcgttcgccg cgatcgtgct
     3181 cgacgacgca gcggcgcgcg gcgtcgggtc cgcgctcgcc gcgcaccacg tcgagctgca
     3241 gggcgaccgg ggccagtggt ggtggcacta cgacgcccag cgcggccacg tcgcactccg
     3301 gtacgccgtg tactcggtcc accagcacgg catggcaccc atggcgctgg ccgccatggc
     3361 acgagccgac ggcggcgact actcggccgc gatcgctgcg agtcgacggt ggatcaccga
     3421 caacgaactc ggatcgtcga tggtcgacgt cgaacggtcg accgtgtggc gcagcgtcga
     3481 gcgagtcgag ccgacggtga tcacgaacgt gcggggcggg ctcgaggcgc tgggcgtggc
     3541 cgaccaccgt gccgccgagg tcacgctcaa ccgtgagacg cgcccctacg agtgggcgtg
     3601 gtgcctctac gccgctgcga tcgagaacga cctccccagg ggcacgtccc ttgtctgacc
     3661 ccgccgccga ggttccggcc gctgcgctgc cgtcggccgc tcccgtcgac gtcttcgact
     3721 ggcaactcgc cgcgctgcgg atgcccgagg tcgtcgccga cctccgccgg ccggccgatc
     3781 acaccaggct cgtgatgacc gtcaacgtgg cgctgatgtg gatggcggcg cgcgacgacc
     3841 gcctcggcca catcgtgcag gacgccgcaa tccgcgtcgc cgacggtatg ggcgtgatct
     3901 ggacctggcg agcgctcggc cgccgactgc cggagcggat cgccggtatc gacctcatgg
     3961 tgcagctcat cgacgccgcg gcggccgacg gccgctcgat ctacctgctc ggggcgcagc
     4021 agcacgtgat cgaccagctc cggcacgtgc tgctccaacg gaatccgtcg ctgatcatcg
     4081 ccggctcccg caacggctac ttcaccgacc aggagcatca cgacgtcgtg tccgacgtga
     4141 agcggtcggg cgccgacctc ctcttcatcg ggatgccgtc accgttcaag gaggtgttcg
     4201 ccgccgatca cctcgacgag ttcggcgccg atctggtgat gggcgtcggt ggcagcttcg
     4261 acgtcatctc ggggttcgtg ccccgggccc cgtcgatgct gcaacgcacc ggcttcgaat
     4321 gggcctggcg cctcgcctgc gagccgcgtc gcatgtggaa acgctacgcc gtcaccaacg
     4381 cgtgggtcgc acgacagctg gtcacgagga tggtccgccg gcgccgtcgc cggccggacc
     4441 cgtcctcctg acctgtcacc gcagaccgtc ggtcagcccg tgtagctgac caactccgcc
     4501 tggacccggg tggccccggt tgccttggtc gacgtcacgc tctcgacctt cacggccgca
     4561 gtggtcgtgg tcgtcgtcgc ggtcgcgtcg gcaaccttcg ccgtcgtggt ggtcgacttc
     4621 ggtgccgtgg tcgtcgtggt cgacttcggt gccgtggtgg tcgtgctcgt ggtgggcgca
     4681 gccgtcgtgg tcgtcgacgt cgtggtcgtc gtggtgggag cggtcaccgt gatcgtcacc
     4741 tggctggtga gtccctgacc gtcgcagaag cgggagatgg tgtacgtacc cggcgtgtcg
     4801 aacacgaacg tgccggtcgc gcccttgatg aagcccgcac ccttcgtctc ctggtgggtg
     4861 tcaccgctcg aggcggacga gatgacgtcg ccgtaggtgg gctcgccctc gctgtcgagg
     4921 cacggctcgc cgaggctgac gttgacgctg ccgccggcag gaatggtcgt gtcggacgcc
     4981 gagatcgacg ggaccgcggc accggccgac atctgcagac cgaggactgc gagcgcactc
     5041 accgagacgg ctgcggccgt ctgtcgaagt agtttcatgg aaatccctcc agggaagaag
     5101 tggcggtcga ggaccaaccg gccgtgtcgc acggtacgga ggcaacgtcc gcgttaccag
     5161 tctcttgtgc cgtttcaggc acttttgctc gcagcagcca gcaaaccttc cgggggatcc
     5221 gggaggcgtg ggactcagtc tgccaggggt gccggggtgg ggcggaccat ccccggggag
     5281 ggcgacgccg gatcgctgaa caaggtgacc aaccgacggg cttcggtcag ggagtcgaag
     5341 tcggcgcgaa cagtggcgcg acccacttca ccgaggcgcc gccgcaggtc ggggtcggct
     5401 gcgagttcgg cgatcgcggc ttcgagcgcc acggcgtcgc ccggtgcgac caccttgcca
     5461 ttgcgcccgt cgcggaccag ttccgtcacg ccacccacgt tggtggccac gaccggcaac
     5521 gtcatggcca tcgcctccat cagcgagacc gggacccctt cggcgaagct cggcagcacg
     5581 aacacgtcgg actcgcggag atgctgttgc acctgctcct ggttgcggta gccggcgaag
     5641 gtgaccgagg aggcgatgcc gagctcgacc gaccgacgtt ccagttccga acgttggggg
     5701 ccatcgccga cgagggtcag gtgcaggtcg ttgccggcgg cgacgaggga cgcgacggcg
     5761 tcgaacagca ccaccacccc cttgaccgcg tcgagccgac cgacgaacaa cagctggcag
     5821 cggccgtcgg acgcggtggc gtcggcgacg tcggcctcgt ccacaccgca gtgcacgatg
     5881 cggatcctgg gccagacctc cgaggggctg aacaggcacg cctgggaccg agcgaagttg
     5941 ctgatcgtcg cgcagaacgc agcggtctcc agcttggtgc cgaggtgcca ctcgttgggc
     6001 tcgaagaaga tcgcggggcc gtgaagcgtg aagctgtagg tcgtgccgct gagctccgag
     6061 gcgagcatgg ccacggtgca actcgagtcg gcgaagtggt tgtggaggtg gtcgacatca
     6121 cgttcggcca tcagctcggc gaggactgca gcctcgacga agtacgccgc ctgcttggcg
     6181 gcgccacgtg ggccgagccg acgcgtcctc acggcgagcc gaagcgccgc gaggaaccgg
     6241 cgtggcgacc ggcgcaacgc ccgggccagc actccagcgt aggacgacga cggctgctcg
     6301 aggaggtagg tggtcaggtc gcgctcgctg cgctggcgct cacccaccat gtgctcgtca
     6361 cccggacgcc gcacggcgaa ccgttcgacg tccaccccac accgttccac ggcgaggatc
     6421 tcgcgttgga tgaacgtgtc ggtggcccgg gggtactcgc cggtgaggta cgcgaccttc
     6481 acgccgagcc ggcccgttcg acgacgaggt cggtgtcgct caggctccgg ctgaacgcct
     6541 gagcgagggt gtactgcggc gcccacccga gcaggcggcg agcgcggtcg ttgttgtacc
     6601 ggaagggttt gaaccggccg tcgagcttct cgggaacgac gatgccgggg aatttggccc
     6661 gaccgtcgag gaagcggttg ttgaccgccg agatcgtggt cgccgccaac ttcatcagcg
     6721 gccagggcac cgggatcgac gacgggatct cggtctgccg ttcgagggca gcgacgtact
     6781 cggcctgcgt ggggaggtcg tcgtcgacga tgttgatcgt ctcactggcg accccgggcg
     6841 catcgatcgc tgcgaccatc gcggcgacgc agttctcgac gtaggtcatc ggcagggttg
     6901 cccggctgcc gatgcgaagg aacagcggtc cgagctcggc gcccagcaac gcgtgccaca
     6961 ggttgtcgcg gccgtagatc atgcccgggc ggaggatgac gacctcgcgg cgctccatct
     7021 cggcccggta gagctgctct tggaggagct tggtctgcgc gtactcgtcg cgcctggtgg
     7081 ggtggaggtc gatcggcgtc gtctcgtcga tgaccgaacc cgccttgagg tgcgagtagt
     7141 cgtacaccga gaacgtgctc acggcgacga ggcgcgtgac cggggagccg tcgatcgccg
     7201 ccaacaggtt ctcggtgctg agcaccgtgc cggcgaactg ggtggcgaaa tcgccggact
     7261 tggccgcggc gagatggatg acgacgtcga tctcgtcgaa caggccgtcg agcgagcggc
     7321 ggtcacgaag gtcggcccgg gcaagggtga ggcgggggtg atcggcaagc gctgcgaacg
     7381 gagccttcga ggccgggcgg acgagcgcgg tcacgtcgtg gcctgcgtgc agcgcctcgg
     7441 ccacggtgta ggcgccgacg aacccggtcg agccggtgac gaggaccctc acgacaccac
     7501 ctccggcgct gcgcccaccg atcgaccggc gaccagccga tcgatcaggc ggctcgtcgc
     7561 gagcatgtcg gcgggcgtga ccggaagttc cgtcccggct gcgagcgcgg tgtaggtcag
     7621 ggcgaggaat cgctcgagac cgacgtacgg cccctgctgc atgatcttgc gcttcagcga
     7681 gcggggcccg ccggcgaaca tcgtcgcgcc acccaccatc atgttgacga tcggcgacag
     7741 ctgtttgccg ccgggacgcg ggatcacccg cgacacacgc ccggagaaca gctcgacctg
     7801 ggcgtagcct tcggtgccac gaaccgtgat ctcgaagcag tcgggtgcga gaagcgccga
     7861 gaaccggatt cgggcgtgaa ccgcaccgtc ggggccgtcg cccacgagga tcgagtcgag
     7921 gtcgtcgacc gccacatggg ccacaccacc gtggttgctc caacggctgt cgacggtgag
     7981 ccactcgacg ccaggggtga agttgtccac cagatacgtg aggtgcgtga tgaagtcgtg
     8041 gatgacgccg gccgggagtt ggtggatcgg actcggaagg ttcgggtcgc cgaaccttcc
     8101 gtcgggatcg ttcacgggca ggcccatccg gacctcgacc tcgccgaccc ggccaagcac
     8161 gccggactcc acgagcgagt cgagctccag aacctcgggg ttgaaccggt agttgtggtt
     8221 ctcgcagatc cgccgaccgg catcgtcggc gaatgccagg agccgctcga ggtcggcagc
     8281 gcccaacgtg atcggcttct cgcagatcac gtcggcaccg gcggcgaggc acagttcgga
     8341 cagcgcgacg tgggtcgtcg gaggtgtgag cacgtgcacg acctcgggcc gcgctccggc
     8401 gagcatctca tcgacggtcg cgtaggcggt ggtgccgtag gtggtcgccg cgtgggagac
     8461 ggcgatcggt gacatgtcgc agacgccgac gaggtcggca cgaggtgagc cggcgaggaa
     8521 ccgcaggtgc tccttggaga tcccaccggt gccgatcacg gcggcacgca gccgcggtcc
     8581 agtaccgctc tcgggttcca ccatctactt cgctccactc acgtcggggt tgcggtcatg
     8641 ttacggagca acccaccgaa cgcaatcacc agtcgcgcga tcactccctc gggaggcgct
     8701 gtcggggcgg cgtgtcgagc gtcgccgacg agattgggcc ggacgcccgg ttgggagaca
     8761 tcgacccgct ctgtaccatc acatcgatga agcgggcgac atctcacgtt gacgggttga
     8821 cccgcgtcga gctcttcggg ttggacttcg tcgacgacac ttcgatcgcc cgggttgccg
     8881 atctcgtcct cgacagcacc cctgacgacg tcgagggcca cccggtgatc gtgaccccca
     8941 ataccgacct cctcctgcag gtcaccaaga cgcccgacgc ccgcatccgc gagttcttcg
     9001 ccaaggcctg gtgtgtcctg cccgacggcc agccgatcgt gtgggcgagc cgaatcctcg
     9061 gctcgcggct gcgtttccgg ctcaccggct cggacctgtt cgccgacctg tggcctcgac
     9121 tgaccgcccg ggccaccccg gtgttcgtgc tcgccccgag cgcagcggtc gcccgcggac
     9181 tcgaggagtc gaaccccgcc gcagcgacgc tggtggcgcc gatgctcgac gtcgggtcac
     9241 gatccgacct cgtggacctc gcggcccagt gcgtcgccga gatcaccgcg gtcgcggcga
     9301 aggtggtgat cctcagcctc gggcacccca aggacgtgat gatcgccgac gagatcctcc
     9361 gacagctgga cgcgtcaccc accgccctcc cgttcatcct ctgtctcggc ggctcggccg
     9421 agatgttcct cggcatccgc aagcgcgccc ccctctgggt gcagaagtgc tcggccgaat
     9481 ggctctaccg cttcgcccag gaaccacgcc gcctgttcca ccgctatttc gtccgtgaca
     9541 tcggcttcat cccgatcgtg tggcgcgagt accaggcgaa gcgccgcaac gatgccgccg
     9601 cgggccaccg cgcgtgagct cgaggtccga ccatccggtc gtgtccgtcg tgatgccgat
     9661 cggcggcatc gacgagttcc ttccccagca gctcgacgcg ctcgctgaac aagaattcga
     9721 ccagccgtgg gagctcgtgc tgtcgatgaa ccagcccggc acgacccatg atcccgccgt
     9781 cgcagcgcct cgcctccacc ccctcgccac gtcgctgatc gtcgactcgt ccgacgagcg
     9841 aagtgctgcg cacgcacgca acgtgggcgc ccgggccgcc tcggccgatc tgttggcctt
     9901 ctgcgacgcc gacgacatcg tcgagcccac ctggctccgc gccctggtcg agtcgctcgg
     9961 ctcacatgcc gcagtcgggg gctacctcga cgaacgacgc ctctccccgc ccggacagga
    10021 gcgctggcgc ccaccggcga cgcccgacga gctgccctcc tacctcgggc accgctaccc
    10081 cgtctcggcc aacatggcaa cgacccgcga ggccttcgac gccgtcgacg gcttcgtcga
    10141 gggcctgacg cgttgcgagg acatcgcgtt cggctgggcg ctcgccgacg cgggcatcga
    10201 cctggcgtac gcgcccgacg cggtcgtcca ctaccgccac cggccgggcc tgtggaccat
    10261 ggtccgtcaa caccactact acggcatcgg catgaccgaa gtcatcgaac gacacggcct
    10321 tcccggcggc gcccagccgt ccggactctt gcaggcgaac aaacaacgcg tgacacaacg
    10381 ctcggtcatc cacatactgc gacgcggagc gatcgcatcg ggccgactgc gcgggctggc
    10441 ccaggaacgc ctgactcgac gccatcgcac ccaagccgca agatagtcag ttcattcgga
    10501 gcgccgatcc ggcgctccaa cccgaaaggt aagagcgtgg agtggtcctt catcgtcgcc
    10561 gcagttcggc gacggatccg gttcgtcgtg ctgtgcgcca tcctcggcgc ggtgccgggg
    10621 ctcctcagca tcgccaactc ggtcagctcg tacgaggccg tcgcgaccat cggcatcgca
    10681 acgccgacct ccggcgccgg ggctgcggtc gccgccagcc agcccgaccg ctacatcacc
    10741 agccagctgt cggtcgtcac gagcaccgac acgctcgaca aggcggccaa gaagctgggc
    10801 ggcggcgtca ccgcgaagca gatcgcggag cagctgaccg tcgagcagga accgtccgcc
    10861 gacatcatca ccgtgaaggc gactgcgagc cggccaaagg cggccgccgc catcgccaac
    10921 gccattgccg acgtctacat caccgaggct gcctctcgtg aggccgccgc acgtgctccc
    10981 cggatccaga cactccagga caagctcgac gcgatcacga cgcagctgac cacacccgac
    11041 accggcatct acgctcagat cacggcggcg atgaaggacc tgatcgccaa ggccaacgag
    11101 gccggctcca ccggctacgt ccccagcgag tcgctcgtcg tccccggcct cgtgcagcag
    11161 cgtgatgccc tgaacgccga gtacaaccgc gtcctgcagg ccaagaacga gctcgagctc
    11221 agcgtcgacg accagtcggt gtcatcgctc atcgagcggg ccacggcgcc ctcgtcaccc
    11281 acgcagacga agggcaagat catcctcgcc ggtggcacgg tcctcggcgg tctcatcggc
    11341 gtcgcggtgg cgctggtgct cgcccagctg tcgtccaagg tgctcgacga ggtgtcggtc
    11401 gcccagatgt tgggcacgac ggtggtcggg gcgttccccc gcaaccgggc gctgctcgac
    11461 aacccgctcg ccgcgctcaa gaccacgccg gccgatgccc gcgagacgat ggaacggctg
    11521 agcgtccgcg ccgaggcgat ggccgatccc gagatcgacc acgcgctcac catcgccgtc
    11581 gtcggcacgc agccggccgc cggcacgacc acgctcacgc tcgcgctcgc ccggcggctc
    11641 agccgctccg ggtactcggt cgtgatcgtc gacggcgacc ttcgcaccca gaccgtgtcg
    11701 gagatcttcg acaccgaccc gtccaccggc atcagcgcgg tcctcgccgc cgaggtcaag
    11761 aagccgacga agccggtcaa ctacttcacc gacaccgacc ttccccgcgt cgaggtgctc
    11821 ggcgtcggca agatcgaacg gcgtgaagcg ttgcgccgtg acgccatcgg tccgatcctc
    11881 gaggtggccc gcgagcgcgg ccagatcgtc ctctacgacg gcggcagcct catgggctcg
    11941 gccctcaccc tcttcgcgag caaggccgcc gacgtgatcg tgctcgccgt tccgctgctc
    12001 gagcaggacc aggagatgct gagcgaggtc gccgacaacc tcccgaccga gcggagccgg
    12061 gtgctcgccg tcaccacggc gcccgtgcgc cgtcgtcatc gtcgccccga caccgccgag
    12121 gccgagccca aggactagcg gtgaaccgca cgctcggtta ccggccgggg ctcgacggcg
    12181 tgcgcgccgt ggccatcggc ctcgtcctcc tcaatcacac cggtctgtcg atcttcctcg
    12241 gcgggggcaa cggcgtcatc gtcttcttcg tgctgagtgg cttcctgatc acgaagctca
    12301 tgctcgagga gtgggaccgg agcggaacga tctcggtcgg cgggttctac gggcggcgcg
    12361 ccgttcgcat cctcccggct ccgacggtga tgctcgtcgt gctgctcgcc gccagctggg
    12421 cgatcaccga gacgccgtcg gagcacagct acctcctgaa ggagatcctg ctcgccgcga
    12481 cctatctcac gaacatgaag ccgctgctgc tgaacgacgt gttcgtcaac ggcgccgtct
    12541 acgacgggtt cctcgggcac atgtggagcc tcgcggtgga ggagcagttc tacctcgtct
    12601 ggccgttgac gatgatcgcc ctcgccctgc ccagccgcaa cgcccggcgg tcggtcaccg
    12661 cgatcgtcgg cgtggcagtg gcgatcgccg tcgtccggct cgggctgtac taccttccgg
    12721 gcctcgaccc cgacctcgcc tcgatctcgc tcttcagctt cgacgggttc gcgctcggcg
    12781 ccgcgatggc cttcgcgttc catcgcggca ccttcccggc gttcgaacgg ctcctgtccc
    12841 gaccggcggt tcccgtcctc gcgatcgcgg tgctcgtcgg tgatctgctg gcggggcggg
    12901 ccacccacga gatctcctcg ctctacgtcg tgtacacctc ggtcgcggca gcggcgctca
    12961 tcgggcacct gttcgtggca ccggactcgt ttgcctctcg ctgggcgtcg ttgccaccgg
    13021 tggtgctcat cgggaagctg tcgtactcca tctacctctg gcacaacccg atctggacgt
    13081 t
//
LOCUS       DAOSOA010000057        12970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4823_length_12970_cov_7.859543, whole genome shotgun sequence.
ACCESSION   DAOSOA010000057 DAOSOA010000000
VERSION     DAOSOA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12970
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4823_length_12970_cov_7.859543"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKY13_05590"
     CDS             complement(<1..500)
                     /locus_tag="PKY13_05590"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT79437.1"
                     /translation="MTNTVERPSLRPDWRRSGLVLLVGTLALMWLVQIVDEVALNDRF
                     EGDGIHPRHLDGLLGIVWSPFLHSGFAHLLANSIPFLVLGGLVVAHGRIRWIQVTVFV
                     IVVGGALTWLLGRTANHIGASGLVFGYFGYLIGSAFVRRNLTAIVTAIIAVVVYGGLI
                     FGFVPR"
     gene            complement(569..2470)
                     /locus_tag="PKY13_05595"
     CDS             complement(569..2470)
                     /locus_tag="PKY13_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT79438.1"
                     /translation="MSIDLRRSLAGGLVALTAVVALSGCFPDPQPLPPTETTTTTTST
                     TTTTTTSTTTTTTAPAPVFTVKINEIESSGGTPGDWVELINLGPDAADVSGWTVVDND
                     ITHTPAALPAGTVIPAGGYYVVEESLLGFGLGGADSATLANSGGTVDSYSWTAHASST
                     YGRCPNGTGAFATTASSTKASANLCQTAATTVKINEVESSGGTPGDWIELINTGANAA
                     DISGWTVLDNDDTHTPSTIPAGTVIAAGGYYIVEESALGFGLGSADSARVFDTTATIV
                     DSYSWTAHAALTWGRCPDGTGAFGATSSTKGAANACVVGPTTTPWPGGSVIQTVDVAN
                     TFGTNMSGLIYEGSGTATPGTLWAVRNGPGTLHRLISDGTTWAPDSGDWAAGKALRYP
                     NGLGNPDAEGVTFTGAGSAGGIFVATERNNDANGVSRNSILRFDPNTAGTSLTATNEW
                     NLTADLPVVGPNLGMEAITWVPDSELVSEGFVDQVTGTAYNPATYANHGDGLFFIGLE
                     ANGTVYAYALDLVGGGFTKVATISSGFTGVMDLQYDRDLQNLWAVCDDGCQGRSTVLG
                     VNASGAYVPGAIYDRPSSMPNINNEGFAIAAATECVADRKPAFWSDDNNTDGHAIRSG
                     TISCSTPVS"
     gene            2618..3067
                     /locus_tag="PKY13_05600"
     CDS             2618..3067
                     /locus_tag="PKY13_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79439.1"
                     /translation="MPAAFQSDRSWRFEVSRNDLWERISATDDYPTWWPWLRSFDAGD
                     PIEVGATWTCAVSPPLPYMVRFAIRFDRVEPGRLVDTTVTGDVEGTARLILEDRPEGS
                     SARLVSALRPANPLLRGFGMVARPLVEYGHDWILDEGRRQFVERAFP"
     gene            3064..3501
                     /locus_tag="PKY13_05605"
     CDS             3064..3501
                     /locus_tag="PKY13_05605"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT79440.1"
                     /translation="MTVSIGPATAADLLDLRRQVLRDGRDDPPSSHVSDDAPDTVHLA
                     ARDVSGQVVGCVSLVRDSRRFGDVDVDVHLVLMAVAFERQGERIGELLIGAAQAVVSG
                     DGPRIWAAARSTALGFYERCGFRAVGDEFVGAMDLPHRLVVWP"
     gene            3525..4034
                     /locus_tag="PKY13_05610"
     CDS             3525..4034
                     /locus_tag="PKY13_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOT79441.1"
                     /translation="MAVIRDATHADLSAVTALSNALIDTTTTTWTERHETVDERRAWL
                     RKQEGAGYPLLVAEDEKGDLVGFATFGDFRDITKWPGYRFVVEHTVHVRGDQHGTGVG
                     RALMEELFDRARRLGKTQMIAGIDGTNEGSIRFHERLGFVEVARMPSIGFKHNTWLDL
                     VLMQRALVD"
     gene            4082..5593
                     /locus_tag="PKY13_05615"
     CDS             4082..5593
                     /locus_tag="PKY13_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79442.1"
                     /translation="MGREVVTGYCWPESAVGGEQVGLHMSSSGGRPVRVEVARIGADR
                     EVVFDVDAVETGEHETPKDAAATGCGWPVALSIDVDPTWRSGYYEVVMEIDVGDKVRR
                     DRAFFVVRPSADARIVLALATNTWHAYNDFGGPNLYTGGTRVSMQRPMAPGYLYKPPG
                     KGRRVTGTGAPDPQNAAHVGYLMLNHLSGYAGSAGWPDWELPFIEWAERAGFEIAVCT
                     NADLEHHPEVLDGARLYLSVGHDEYWSAGMRDTVEAFIGRGGNAAFFSGNTSLWQVRI
                     EGDDHDVMVGYKGFFKDDPVLGTDREPEVTTFWSDVLVGRPENLMTGVSFTRGGYHRI
                     GRNVSSGLGGYTVHRADHWIFEGTGLGYGDVLGVDATVVGYECDGCVFTYRDGLPYAT
                     GEDGTPSTFEILGTCPTQHFTRETAPRPPKPGEPSELEYIASRVFGTREPEAMDRIRH
                     GHAVLGAYTNDAGATVVTSGSTDWAHGLAARDPQIEQITRNVLGRLGGDGSEG"
     gene            5590..6366
                     /locus_tag="PKY13_05620"
     CDS             5590..6366
                     /locus_tag="PKY13_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT79443.1"
                     /translation="MSANWRHPGRTGAIIYALLAAVFGGLVTATSRLQVERQRGRAEV
                     ARRLPDGAVIVISNHTSYADGVLLVLACRRQGRSLRMLATAGVFKVPVVGWLGRRLGF
                     IPVSRGTKDAAGSLDAAVEALSHGEAVGLYPEGRLTRDPAMWPERAKTGAVRLALRSG
                     APIVPVAMVGAHEVVGRRRILLRLMANVIRRPRVEVRIGAPIDALGLAGGVTDPPPDV
                     VREIADSTMAELIELVAELRGEEPEHPTGVPRGEDPSPQP"
     gene            6447..7562
                     /locus_tag="PKY13_05625"
     CDS             6447..7562
                     /locus_tag="PKY13_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79444.1"
                     /translation="MRWRTFTTIATVLSGFAVTLAGCTPTNTAPPSITTSDVSVVATP
                     AADQSAPCLDSDSALNTFANSDATSFVVTINVIAPLCTPLDAKAVIYAMPNNGQAWPQ
                     ELFKVVPFTLSQAGTVTVTFTKGCGAVQFDVLTGSTPQTIDNPLQHGQLIDPVAAQQW
                     FGAPCPTGTTTTTEATTSTTSSTSTTTEPTTTTTSTSTTTTEPPTTTTTSTTSTTSTT
                     TTTSTTVPPTTTTTSTTTTTAPPLLPLVYTGVADKTAGCYSGAAGDDTFHQVLGTDGY
                     WDFYFQGCSAFATETIALDDPPGGPFANAYEPPTGYKCRMSIDGSADGPTPWYSGADP
                     QVGSVADTGGLIFFQSSSSWSSDWPNPTTWRITCDPI"
     gene            7686..8726
                     /locus_tag="PKY13_05630"
     CDS             7686..8726
                     /locus_tag="PKY13_05630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT79445.1"
                     /translation="MTSSGSDVEFGIDTFGGITKGADGRPLHHAEVIRNIVEEGVLAD
                     EVGLDFIGIGEHHRRDFAVSSPEMVLAAIAARTERIRLGSAVTVLSSDDPVRVFERFS
                     TLDAVSNGRAEVVVGRGSFTESFPLFGLDLARYEELFEDRLGLFAEIVEERPVTWSGS
                     TRASLTSTSIYPPTERGLTVWVGVGGSPESVVRAARFHFGLFLAIIGGAPERFRSYVD
                     LFERAQDEFGVERRPVAVHSPGFVGATDEEAYEAVFDGWAEMRTRIGAERGWPPPTSG
                     DFEREIEHGSLYVGSPETVARKVARTITALGVDRFDLKYDSGSSSHEAMLGSIELFGR
                     KVVPMVKDILSS"
     gene            complement(9292..9972)
                     /locus_tag="PKY13_05635"
     CDS             complement(9292..9972)
                     /locus_tag="PKY13_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79446.1"
                     /translation="MRTQRYWRRLAAVVAIGVATVTTGCGSEARVDVAAGGAANDMSS
                     RAADPISACSGSEYALGDGTPVDWTEVAARFDSDGPFRLPVKPKWLPAEGYCGAILSA
                     ISAPPAPPDGATAVDPGVNRFVLAASATSMDDVRYTNAVLTAMYPASILPVYDGKGNL
                     DGFITPGLGQVGLDTFATAADAAASPEVKKATSDLVAGCSSKSCDSMIEAAIESGNES
                     ILGSPVQP"
     gene            complement(9969..10484)
                     /locus_tag="PKY13_05640"
     CDS             complement(9969..10484)
                     /locus_tag="PKY13_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79447.1"
                     /translation="MNKTTGVRRLTALVAITAILVATSSCVNRQGWFAAFGDTRACYG
                     VALETRTTKLDGGAASWYSAQNTCNYASQLQLATFVQVSVTIQAWIANPGIWANCTLT
                     KTVANNTGTITFDFNETWGGDQFNGGVCGGGPFSGDPQAGVLFATNAGHVVKVNGTNQ
                     FTNQAVTEVAS"
     gene            11361..12533
                     /locus_tag="PKY13_05645"
     CDS             11361..12533
                     /locus_tag="PKY13_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79448.1"
                     /translation="MKNRRTWAARIAVIACAGILTTACQPSDVYNLPRARSGAKCTSP
                     GSFARDTTNVLQCSPKRRWAVNMPISRAVTLIDAYNRSITPTEIVRLGAPQIVIAGSG
                     AADAVVIVRNADGGPVKDAVVQFTQSGTTSLNWASPTETRTGADGKASVTFTPSTSIG
                     GTRVTATVQGTGLSTIYSIDTRAAEPATFTITGSTLVTMNLRSSVTLKARLTDRFGNP
                     VWNEPVDVLTDSEDFSFQPLTSGTQPDRTDGYGEYSGVLSSSAVAGQFGVAFVAGPDD
                     REVAATWDVDVLPGAAYSVHLTSSSLTAPVNTPLPGPIVVTVSDSDGNPVSGIPVTLD
                     IATDTSSATGSFGATSGVTDALGHVTTTFTCGATAGIAYVGAHAAGINGYGPITCT"
BASE COUNT         2040 a         4153 c         4576 g         2201 t
ORIGIN      
        1 cttcggggca cgaatccgaa gatcagcccg ccgtagacga cgacggcgat gatcgcggtg
       61 acgatcgccg tgaggttgcg gcggacgaac gccgatccga tgaggtagcc gaagtacccg
      121 aacacgaggc cgctggcgcc gatgtggttc gccgtgcgac cgagcagcca cgtgagcgcg
      181 ccgccgacga cgatcacgaa gacggtcacc tggatccagc ggatccggcc gtgggcgacc
      241 accaggccac ccagcacgag gaacgggatg ctgttggcga ggaggtgagc gaacccgctg
      301 tggaggaacg gggaccagac gatcccgagg agtccgtcga ggtgccgggg gtggatgccg
      361 tcgccttcga agcggtcgtt caacgccacc tcgtcgacga tctggacgag ccacatcagc
      421 gcaagggtgc cgacgaggag caccaggccc gatcgccgcc agtccggtcg gagggagggg
      481 cgttcgaccg tgttcgtcat cgggcccagc gtcgcacgcg acgccgggcc cgtcgatgca
      541 cccaggggtg ctgttcagct gtcgccggtc aggagaccgg cgtggagcag gagatcgtgc
      601 cggagcggat ggcgtggccg tcggtgttgt tgtcgtcgga ccagaacgcc ggcttgcggt
      661 cggcgacgca ctcggtggcg gcggcgatgg cgaagccctc gttgttgatg ttcggcatcg
      721 acgacgggcg gtcgtagatg gcccccggca cgtaggcacc ggaggcgttc acgccgagca
      781 ccgtcgagcg gccctggcag ccgtcgtcac acacggccca caggttctgg aggtcgcggt
      841 cgtactgcag gtccatcacg cccgtgaatc cggagctgat ggtggcgacc ttggtgaagc
      901 caccgccgac gaggtcgagt gcgtaggcgt acaccgtgcc gttggcctcg agtccgatga
      961 agaacagccc gtcaccgtgg ttggcgtagg tggcggggtt gtacgccgtc ccggtgacct
     1021 gatcgacgaa cccttccgag acgagctcgc tgtcgggcac ccaggtgatc gcctccatgc
     1081 cgaggttggg gccgaccacg ggaaggtcgg cggtgaggtt ccactcattg gtggcggtca
     1141 gcgacgtgcc ggcggtgttc gggtcgaagc ggaggatgct gttgcgactc acgccgttcg
     1201 cgtcgttgtt gcgttcggtg gcgacgaaga tgccgccggc cgacccggcg ccggtgaacg
     1261 tgacgccctc ggcgtcgggg ttgccgaggc cgttggggta gcggagggcc ttgccggcgg
     1321 cccagtcacc ggagtcgggc gcccaagtgg tgccgtcgga gatgagacgg tggagcgtgc
     1381 cgggtccgtt cctcacggcc cagagcgtgc cgggggttgc cgttccggaa ccctcgtaga
     1441 tcaggccgct catgttcgtg ccgaaggtgt tggcgacgtc gacggtctgg ataacggagc
     1501 cgccgggcca cggggtcgtg gtgggtccga cgacgcaggc gttggcggcg cccttcgtgg
     1561 agctcgtcgc gccgaacgct ccggttccgt cggggcagcg gccccacgtg agggcggcgt
     1621 gggcggtcca gctgtagctg tccacgatcg tggcggtggt gtcgaacacc cgcgccgagt
     1681 cggcgctccc gagtccgaag ccgagggcgg attcctcgac gatgtagtag ccgcccgctg
     1741 cgatgacggt gccggcgggg atggtcgacg gggtgtgggt gtcgtcgttg tcgagcacgg
     1801 tccagcccga gatgtcggcg gcgttggcgc cggtgttgat cagctcgatc cagtcgccgg
     1861 gggtcccgcc gctcgactcg acctcgttga tcttcacggt ggtcgccgcg gtctggcaga
     1921 ggtttgccga ggccttggtg gacgaggcgg tcgtggcgaa ggcgcccgtg ccgttggggc
     1981 aacggccgta ggtcgacgac gcgtgggccg tccacgagta cgagtcgacg gtgccaccgc
     2041 tgttggccag cgtggccgag tcggcgccgc cgagcccgaa gcccagcagc gactcctcga
     2101 cgacgtagta gccgccggcg ggaatcacgg tcccggcggg gagggccgca ggggtgtggg
     2161 tgatgtcgtt gtcgacgacg gtccagcccg agacgtcggc ggcgtcgggg ccgaggttga
     2221 tcagttcgac ccagtcgccg ggggttccgc cgctggactc gatctcgttg atcttcacgg
     2281 tgaacaccgg cgccggggcc gtcgtggtgg tggtggtgga cgtcgtggtg gtggtggtgg
     2341 tggacgtcgt cgtggtggtc gtctcggtcg gtggcagggg ctgggggtcg gggaaacagc
     2401 cggacagtgc aaccacggcg gtgagggcaa cgaggcctcc cgcgaggctg cggcgcagat
     2461 cgatggacat tggggatccc ttcagctgtc ctgcggagag caggggcggt gattggggcg
     2521 aacctacgga tcggggtgga ccgctcggcg aacgtcaggc caacgggcgt tgaagcttcg
     2581 gcgagcggtc ggtgcgttcg cggacgagac tgggtcggtg cctgccgcct tccagtccga
     2641 tcggagctgg cgattcgagg tgtcccggaa cgatctgtgg gaacggatca gcgcgaccga
     2701 cgactatccg acgtggtggc cgtggctgcg gtcgttcgac gccggcgacc ccatcgaggt
     2761 gggagcaacg tggacgtgcg cggtctcacc cccgcttccc tacatggtca ggttcgcgat
     2821 ccgcttcgac cgggtcgagc ccggtcggtt ggtcgacacc accgtcaccg gcgacgtcga
     2881 ggggaccgcc aggttgatcc tggaggaccg acccgagggc tcgagcgccc ggctggtgtc
     2941 ggcgcttcgc ccggcgaatc cgctgctgcg tgggttcggc atggtggctc gaccgctggt
     3001 ggagtacggg cacgactgga tcctggacga gggtcggagg cagttcgtcg agcgggcgtt
     3061 cccgtgacgg tgtcgatcgg acccgcgacc gcggccgacc tcctcgacct tcggcgacag
     3121 gtgctccgtg acggacgcga cgatccgccg agttcgcacg tatcggacga cgcccccgac
     3181 acggtccacc tcgcggcgcg cgacgtatcg ggccaagtcg tcggctgtgt gtccctcgta
     3241 cgcgattctc ggcggttcgg cgacgtcgat gtcgacgtcc atctggtcct gatggcggtg
     3301 gcgttcgaac ggcagggcga gcgcatcggt gagctcctca tcggggccgc ccaggcagtg
     3361 gtgtcgggcg acggcccgcg catctgggcg gcggctcgga gtaccgcgct cggcttctac
     3421 gagcggtgtg gcttccgggc ggtcggtgat gagttcgtgg gggccatgga cctcccgcac
     3481 cgcctggtgg tgtggccctg agcgtcgagg tcctaccctt cggcatggct gtgatccgtg
     3541 acgcgaccca cgccgacctg tcggcggtca ctgcgttgag caacgcgctg atcgacacga
     3601 cgacgacgac gtggaccgag cgtcacgaga cggtcgacga gcgacgggca tggctccgaa
     3661 agcaagaggg tgccggctat ccgctgctcg tggccgagga cgagaagggt gacctggtcg
     3721 gattcgcgac gttcggcgac ttccgcgaca tcaccaagtg gcctggatac cggttcgtcg
     3781 tcgagcacac cgtgcatgtg cgtggcgatc agcacggcac aggcgtgggc cgggcgctga
     3841 tggaggagct gttcgatcgg gctcgtcggc tcggcaagac ccagatgatc gccggcatcg
     3901 acgggacgaa cgagggctcg atccggttcc acgagcggct cggattcgtc gaggtcgccc
     3961 ggatgccgag catcggcttc aagcacaaca cgtggctcga cctcgtgctg atgcagcggg
     4021 cgctcgtcga ctgatggacg ggaccgtccc ggcccgaaat tgctcgggat agcttcgggc
     4081 aatgggacgt gaggtggtga cggggtactg ctggccggag tcggccgtgg gcggtgagca
     4141 ggtggggctc cacatgtcgt cgtcgggcgg gcgaccggta cgggtcgagg tggcacggat
     4201 cggcgccgac cgcgaggtcg tgttcgatgt cgatgccgtg gagaccgggg agcacgagac
     4261 gccgaaggat gctgcggcga cgggctgcgg ttggccggtg gcgctctcga tcgacgtcga
     4321 cccgacgtgg cgatcgggtt actacgaggt cgtgatggag atcgacgtcg gtgacaaggt
     4381 gcgtcgcgac cgtgcgttct tcgttgtgcg accgtcggcc gacgcgcgca tcgtgttggc
     4441 gctcgcgacg aacacgtggc acgcctacaa cgacttcggc gggccgaacc tgtacaccgg
     4501 cggcacccgg gtctcgatgc agcggccgat ggccccgggg tacctctaca agccgcccgg
     4561 caaggggcga cgtgtcacgg gaacgggcgc tcccgatccg cagaacgccg cgcacgtcgg
     4621 gtacctgatg ctcaaccatc tctcgggcta tgccggatcc gcagggtggc ccgactggga
     4681 gctgccgttc atcgagtggg ccgagcgagc cggcttcgag attgcggtgt gtaccaatgc
     4741 cgatctcgaa caccaccccg aggtgctcga cggcgcccgg ctctacctgt cggtcggtca
     4801 cgacgagtac tggtcggccg gcatgcgcga caccgtcgaa gcgttcatcg gtcgcggcgg
     4861 caacgccgcg ttcttctccg gcaacacgtc gttgtggcag gtgcgcatcg agggcgacga
     4921 ccacgacgtg atggtgggct acaaggggtt cttcaaggac gacccggtgc tgggcaccga
     4981 ccgggagccg gaggtcacga cgttctggtc cgacgtgctc gtcggacgcc ccgagaacct
     5041 gatgaccggc gtgtcgttca cccgtggtgg gtatcaccgc atcggacgca acgtctcgtc
     5101 gggcctcggc ggctacaccg tgcaccgtgc cgaccactgg atcttcgagg gaaccggtct
     5161 cggctacggc gacgtcctcg gggtggacgc cacggtggtc ggctacgaat gcgacgggtg
     5221 cgtgttcacg tatcgggacg ggctgcccta cgccaccggc gaggacggga cgccgtcgac
     5281 gttcgagatc ctcggaacgt gtccgaccca gcacttcacg cgtgagaccg ccccgcgtcc
     5341 gccgaagccg ggcgagccga gcgagctgga gtacatcgcc tcgcgggtgt tcggcacccg
     5401 tgagcccgag gcgatggacc gcattcggca cggccatgcg gtcttgggtg cgtacacgaa
     5461 cgacgccggc gcgacggtgg tgacgtcggg gtcgaccgac tgggcgcacg gcctcgcagc
     5521 gcgggaccca cagatcgagc agatcacccg caacgtgctc ggtcgtctgg gtggcgacgg
     5581 gagcgagggg tgagcgcgaa ctggcggcac ccgggccgga ccggggcgat catctacgcg
     5641 ctgctcgcgg cggtgttcgg cgggctcgtc acggcgacga gccgcttgca ggtggagcgc
     5701 caacgcggcc gcgccgaggt ggcgcgccgt cttcccgacg gggcggtcat cgtgatctcc
     5761 aaccacacga gctacgccga cggtgtgctc ttggtgctgg cgtgtcgtcg gcagggccgg
     5821 tcgttgcgga tgttggcgac ggcgggggtg ttcaaggtgc ccgtcgtcgg gtggctcggt
     5881 cgacgtctcg gcttcatccc ggtgtcgcgg ggcacgaagg acgcggcggg ttcgctcgat
     5941 gctgcggtcg aggcgctgtc gcatggcgag gcggtcggct tgtaccccga gggtcggctc
     6001 acgcgtgacc cggcgatgtg gccggagcgg gcgaagacgg gtgcggtccg gttggcgttg
     6061 cggagcggtg cgccgatcgt gcccgtcgcg atggtcggcg cgcacgaggt ggtcggtcgg
     6121 cggcggatcc tgctgcggct gatggcgaac gtgatccgcc gtccgcgcgt cgaggtgagg
     6181 atcggtgcgc cgatcgatgc cctgggcctc gccggtgggg tgaccgaccc gccgccggac
     6241 gtggtccgcg agattgccga ttcgacgatg gctgagctga tcgagctcgt ggccgagctg
     6301 cgaggcgagg agcccgaaca ccccacgggt gtgcctcgtg gcgaggatcc gtcgccccag
     6361 ccctgagcag ggaaacccgc ccgaatcagg caaatgccca cgcgaatcac gtgctgtggc
     6421 cacatggtgc gaggtacgtt cacgtcatgc gctggagaac gttcacaaca attgccactg
     6481 tcctcagcgg gttcgccgtc acccttgccg gctgcacccc aacgaacacg gccccgccgt
     6541 cgatcaccac gtccgacgtc tcggtcgtcg ccacgccggc ggccgaccag tcggcgccct
     6601 gtctcgattc ggactcggcg ttgaacacgt tcgccaactc cgacgccacg tcgttcgtgg
     6661 tcacgatcaa cgtgatcgcg ccgctctgca cgccgctcga tgccaaggcc gtcatctacg
     6721 cgatgccgaa caacggccag gcgtggccgc aggagctgtt caaggtggtg ccgttcacct
     6781 tgagccaggc cggcacggtc accgtgacgt tcacgaaggg ctgcggtgcg gtgcagttcg
     6841 acgtgctgac cggctcgacg ccgcagacga tcgacaaccc gctgcagcac ggtcagctca
     6901 tcgaccccgt cgctgcccag cagtggttcg gtgctccgtg cccgacgggc accacgacca
     6961 cgaccgaggc gaccacgtcg acgacctcgt ccacctcgac gaccaccgag ccgacgacga
     7021 ccaccacgtc aacgtcgacc accaccacgg agccgccgac gacgaccacc acgtcgacga
     7081 cctcgacgac ctcgacgacg acgaccacct cgaccacggt gccgccgacg acgaccacga
     7141 cgtccaccac aacgaccacg gctcctccgc tcctgcccct cgtctacacg ggcgtggccg
     7201 acaaaaccgc cggttgctac tcgggtgccg ccggtgacga caccttccac caggtgctcg
     7261 gcacggatgg atattgggac ttctacttcc agggctgcag cgctttcgcc accgagacga
     7321 tcgctctgga cgatccgccc ggcggcccgt tcgccaatgc ctacgagccg ccgaccggct
     7381 acaagtgccg catgtcgatc gacggttcgg ctgacggccc gaccccctgg tactccggtg
     7441 ccgacccaca ggtcggttcc gttgccgaca cgggtggcct gatcttcttc cagtcgtcct
     7501 cgtcatggag ttcggactgg ccgaacccga cgacctggcg gatcacctgc gatccgatct
     7561 gaggccgatg atctgagatc cggcgtcgac ggcgcccacc gtacggtggg cgccgttgcg
     7621 cgtctggacg ccccaccggg cgtcggcgag ggaatccgcc gacctcggtc gctgttgcat
     7681 cggccgtgac ttcatcagga tctgacgtcg agttcggcat cgacacgttc ggcggtatca
     7741 ccaagggagc cgacgggcgc cccctccacc atgccgaggt gatccgcaac atcgtcgagg
     7801 aaggcgtgct ggccgacgag gtcggtctcg acttcatcgg catcggcgag catcaccgtc
     7861 gggacttcgc tgtgtcgagc ccggagatgg tgcttgcggc gatcgccgct cggaccgagc
     7921 gcattcggct cggttcggcg gtgacggtgt tgagctccga tgatccggtg cgggtgttcg
     7981 agcgcttctc gacgctcgat gcggtgtcga acggtcgcgc cgaggtcgtg gtgggtcggg
     8041 gatcgttcac cgagtcgttc ccgctcttcg gcctcgacct cgctcgctac gaggagttgt
     8101 tcgaggatcg gctcgggttg ttcgcggaga tcgtcgagga gcgcccggtg acgtggtcgg
     8161 gttcgaccag ggcgtcgttg acgtcgacgt cgatctatcc gccgaccgag cgcgggctga
     8221 cggtgtgggt cggtgtcggc ggcagccctg agtcggtggt ccgggccgcc cgcttccact
     8281 tcggcctgtt cctggcgatc atcggcggtg cacccgagcg gttccggagt tacgtcgacc
     8341 tgttcgagcg ggctcaggac gagttcgggg tcgagcgtcg cccggtcgcc gtgcactcgc
     8401 cgggtttcgt cggggcgacc gatgaggagg cgtacgaggc ggtgttcgac ggctgggccg
     8461 agatgcgtac ccgcatcggt gccgagcgcg gctggccgcc gccgacaagc ggagacttcg
     8521 agcgggagat cgaacacggt tcgctctacg tcggctctcc ggaaacggtg gcgcgcaagg
     8581 tggctcgcac gatcacggcc ctcggggtcg accggttcga cctgaagtac gacagtggat
     8641 cgtcgagcca cgaggcgatg ctgggatcga tcgagctgtt cggccgcaag gtcgtcccca
     8701 tggtgaagga catcctctcg agctgacggc ccggtcggag cgggcgagag tttctcgtgc
     8761 agtcgaagat gcaatcgctc gcgtgctccg cgggtgaggc gcaccatcga gtggttcgcg
     8821 aggacgtggt ccgtgccgtg ttcgcgcagc tcggcctgca ggcgatcagc gacttgttct
     8881 gccgtctcgc gggtcgcgaa caaggcccgc acccgccagg tccggctgcc cacgacgatt
     8941 gctactgcgt atccgtcatc agacgtcggc aatcttgcgc cggcctcgta gaacgccccc
     9001 ggcaggttgt cgcgcgccca ctcgtggcag cggtcgcgca gcactgtgcg ttcggcggcg
     9061 aaccgttccg cccgctggtc agtagcccga tcagtacgta cccccgactg atcgtctcga
     9121 cgcgatctgt gaggtcgtcg cacacgcggt ccaagtccct cgccgagtcc tcgtcgagca
     9181 cgaactggac gatcaaggca gtcaggctcg gcgcaatcga agcagacgac acgggaccat
     9241 gggagggctc ctcgggctcg gggtgccctc ccatggtcat gttgtgcgcg cctacggctg
     9301 aactggtgat ccgagtatcg actcgttgcc ggactcgatc gcggcctcga tcatcgaatc
     9361 gcacgacttg gaggagcacc ctgcgaccag gtccgaggtg gccttcttca cctcgggaga
     9421 tgctgcagcg tcggcggcgg tagcgaacgt gtcgagccca acttgtccga ggcctggagt
     9481 gatgaatccg tcaaggtttc ccttgccgtc gtagacgggc aggatggacg ctgggtacat
     9541 cgcggtgaga accgcattgg tgtaccgaac atcgtccatc gacgtcgctg atgctgcgag
     9601 tacgaatcgg ttcacgccgg gatcgacagc agtggccccg tctggcggcg ccggcggcgc
     9661 cgagatcgct gacaagatcg ctccgcagta accctctgcg gggagccact tgggcttcac
     9721 cgggagacgg aacggaccgt cgctgtcaaa cctcgcggcg acctccgtcc agtccacggg
     9781 tgtgccatcg cccaaggcat actcggagcc ggaacaggcc gaaatcgggt ccgccgctcg
     9841 ggatgacatg tcattcgctg ctcctccggc agctacgtcg accctcgctt ctgagccgca
     9901 tcccgtagtg acggttgcca cgccaatggc gacgaccgcg gccaagcgtc gccagtacct
     9961 ctgcgtcctc atgatgcgac ctccgtaact gcctggttcg tgaattggtt cgttccgttt
    10021 actttgacga cgtgtcccgc gttggtcgcg aacaggacgc cggcctgtgg gtcgccggag
    10081 aacgggcccc cgccacagac gccgccgttg aactggtcgc cgccccacgt ctcgttgaag
    10141 tcgaaagtga tcgtgcctgt gttgttggca acggtcttgg tgagcgtgca attcgcccag
    10201 atgccggggt tggcgatcca ggcctggatc gtgaccgaca cctgaacgaa ggtggcgagc
    10261 tgtaattggc tagcgtagtt gcaggtattc tgtgccgagt accacgatgc cgcgccccca
    10321 tctagtttgg tcgtccgggt ctcgagtgcg acgccgtagc acgctctcgt gtcgccgaaa
    10381 gcagcgaacc atccctgtcg gttcacacat gagcttgtcg cgacgaggat cgcagtgatg
    10441 gcaactaacg ccgtcagcct gcgaactccg gtcgtcttgt tcatagatgc acccctttcg
    10501 gttcggtcga agctgaaatg ctccggttcc tcaaccggag tccaccgagg atgcccaggt
    10561 cgatcaattc gtacttgcct cgacggtgaa cgatgtgaca aagaccagga gacgaatcct
    10621 cactcgcgat agtcaggaca gcgcaatcct gaggcttgcc aacttgtccg accagggtcg
    10681 tcttagccgt cagggaccac cgttccgtcg gactcaggta cgacgatgac gtcgtcgggg
    10741 agtgtggagt tgacccggct ttgttggaca ccagattctg gagcgcatcg ctctggagga
    10801 aggaggcccc aatgggtcgt ccatcgaatt aacccgagga attccggcgt gaagcggtcg
    10861 cactgttccg cagctcggac cggtctcggg tcgaggtagc ccgatcgttg ggcatcagcg
    10921 acggatcgct ggcgtcatgg gtgaaagccg ccgacgaatc cgaaaatccg ggcgcgttgg
    10981 accccaccga acgcgctgac tcgcgggtcg agaacacggt ccgtacccgc caggtccggc
    11041 tgcccacgac gattgctact gcgtatccgt catcagacgg tgacgagaat ccagtgagta
    11101 ggtctgcgaa caggtcgcgg aagccatacg tccggtagcc gatgtgatcc tgcgccgttg
    11161 ccatgccgct acacgatctc cccgagttga cccgtggcag agcgacatcc tgtcagccgc
    11221 tgccagtggg ccgggcccgc gatcgactca cgaacctgcg tggtggagcg ccgtggagga
    11281 gtgaaacgcc gtcgccgaag accttgcggc aaggagcggc ctcgcagaca atcaccatcc
    11341 acctgagaca aggacgaacg ttgaagaacc gacgaacatg ggccgcccgc atcgccgtca
    11401 tcgcctgcgc tgggatcttg accacggcgt gtcagcccag cgacgtctac aacctcccgc
    11461 gggcacggag cggcgccaaa tgcacgagcc ccggctcgtt cgcccgggat acgaccaacg
    11521 tgttgcagtg cagcccgaag cgccgctggg cggtcaacat gcccatcagt cgggccgtca
    11581 cgttgatcga cgcgtacaac cgctccatca ctcccaccga gatcgttcgg ctgggcgccc
    11641 cgcagatcgt gatcgccgga tcgggcgccg ccgacgcagt ggtgatcgtc aggaacgccg
    11701 acggcgggcc ggtgaaggac gccgtcgttc agttcacgca atcgggaacc acgtcactca
    11761 actgggcctc gcccaccgag acgcgcaccg gtgctgacgg gaaggcgtcc gtgaccttca
    11821 cacccagtac ctcgatcggt ggcacccgtg taacggcgac cgtccagggc accggcctgt
    11881 caacgatcta ctcgatcgac acccgcgccg cggagccggc gacattcacc atcaccgggt
    11941 cgacgctcgt cacgatgaac ctccgctcgt ccgtgactct gaaggcccga ctcaccgaca
    12001 ggttcggcaa cccggtgtgg aacgagccgg tcgacgtcct gaccgactcc gaggacttct
    12061 cgttccagcc gctcacgtcc ggaacgcagc cggaccggac cgatgggtac ggcgagtact
    12121 ccggtgtcct ctcctcctca gcggttgccg ggcagttcgg tgttgccttc gtcgcgggtc
    12181 ccgatgaccg cgaggtcgcc gcgacgtggg acgtcgatgt gttgccagga gcggcctact
    12241 ccgtccatct gacgtcgtcg agcctcaccg ctccggtcaa tacaccactc cccgggccga
    12301 tcgtcgtgac cgtgtccgac agtgacggca acccagtgtc gggaattccg gtgacgctcg
    12361 acatcgcgac cgacaccagc tcggccaccg gcagcttcgg cgcgacgagt ggtgtcaccg
    12421 atgcactcgg tcacgtgacc accacgttca cgtgcggcgc caccgctggc atcgcgtacg
    12481 tcggagctca cgctgcaggg atcaacggct acggcccgat cacctgcacc tagtcgattg
    12541 gcccccccgg gatgacggcg ggcggccgat ggaagcgtta ccttccatcg ggtacatgag
    12601 ctcatgagag ctgcgacaac cgaagacctc ggtgcggcag ttcgagccga gcgaacacga
    12661 cgagggatga ctcaaaccga gcttgcttcg ttgtcgggga ccactcgcca gtggatcagc
    12721 gcgttcgagc gggggcgaac ccgagtcgag acggacctgg cgttcggagc acttggcgcc
    12781 atcggactgt cggtcgatct ctcgcctgac ccgcccgagc cggcgcacgg aatgctcacc
    12841 acagttgagg cggcgaggcg tattcgccgg gagctggaca atggcgacac cgagttcgcg
    12901 ctccgcctcc tggctgattg cgtcccatgt cgtggtgtag gtggccacgt gcgttccccg
    12961 gttgctgatg
//
LOCUS       DAOSOA010000058        12842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_4869_length_12842_cov_5.431297, whole genome shotgun sequence.
ACCESSION   DAOSOA010000058 DAOSOA010000000
VERSION     DAOSOA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4869_length_12842_cov_5.431297"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PKY13_05650"
     CDS             <1..976
                     /locus_tag="PKY13_05650"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOT79449.1"
                     /translation="AGGLGGLGDVLGGALGGAGGGGAGGGSLGGVLGGMFGGKGRKGG
                     VVGVLLVLAAVFLLPKIMSGGGFDVSTAGLDNLPGMSGGGALPGSGDVADASLPAADD
                     PDPELTQFANVLITDTNNVWEQQFDEASKRYDRTKLVLFTGGVNTACGQASSDMGPFY
                     CPGDQLVYIDLTFFEELATRFGAAGDFAQAYVIAHEVGHHVQNELGISAQVRRLEEDD
                     PGAANGPDGLSVRTELQADCLAGVWAHTRYERGESDPTKRLDDGDIDEALRAAAGVGD
                     DSIQKSATGRVNPEGFTHGTSAQRSRWFKKGYDSGDSEQCDTFSTDSL"
     gene            1422..2306
                     /locus_tag="PKY13_05655"
     CDS             1422..2306
                     /locus_tag="PKY13_05655"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT79450.1"
                     /translation="MLAYMDVDLSTGLDALLPLVAPLVTGHSDVAIGSRLASGASVAR
                     GPKREFISRSYNLLLHTVFATHFHDAQCGFKALRADAAAALLPQIDDDEWFFDTELLL
                     VAEHNGLRIHEVPVDWVDDPDSRVRVAQTARRDLAGMARMAWRFLRGAGRIDGATYGR
                     AELADQMGRQLVVFGLIGTISTGVSAGLFLALREPLGAVLANVVAVTATVAVNAWANR
                     RFTFGRRGRLGRSSDYLRAGAIYLVGLVVSTFVLSAISPTDHLVELLALLATWSLTTV
                     ARFTVLSHTPITSTEVQP"
     gene            2303..4447
                     /locus_tag="PKY13_05660"
     CDS             2303..4447
                     /locus_tag="PKY13_05660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT79451.1"
                     /translation="MTVADHPVPATARAAVQPPMEPAAAAQQHRSARLRRVVRGRDDD
                     PVWARPALVGLLVGTAVLYLWTLSESGWANAFYSAAVQAGSQSWKAFFYGSSDAANSI
                     TVDKPPMSLWPMALSVRVLGLSSFSILLPQALMGVATVGVLYAAVRRWFSAHAALLAG
                     VVMALTPVAALMFRFNNPDALLTLLMVIAAYFALRAIESGAWKWFVATGVAVGFGFLT
                     KQLQVLLIVPGLGAAHLAFGGNGFWRRIRDLAIAAAAMVVSAGWWIAIVELVPASMRP
                     YVGGSQDNSILELTLGYNGLGRLTGNETGSVGGGNGPGGGMWGETGIGRLFGNEIGGQ
                     ITWLVPAALILLAAVLVVWWRRPRRDLQRASIAVWGSWLVVTGAVFSFMSGIFHAYYT
                     VALAPAVAALVGIGAVTLWQHRTTSPRAHAATMTALAVIAGVTTWWARVLLARAADWN
                     PWISTLVLVAGGTAVGGLLAAAWLRPLWGDRAANWLGVGAAGLALVAGLAGPTAWTLQ
                     TVATPHTGSIVTAGPTVTGGGFGPGRGGFPGGMTGTMPGGLPGGFPGGNPPAIGTTPP
                     GGTTGGFPGGLGARPGGGGGMGGLLDAVTPGAEIVAALSEDASSYTWVAAAVGSNNAS
                     GYQLATELPVMAIGGFNGSDPSPTLAEFQQYVADGKIHWFIGGGGFGGGGFGNQMGGS
                     DASSEIATWVQENFTATTIDGSTLYDLTAPIA"
     gene            4534..4857
                     /locus_tag="PKY13_05665"
     CDS             4534..4857
                     /locus_tag="PKY13_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79452.1"
                     /translation="MGQPVTVIEKQSPTTGVVRFELNRVLTGTGHERYKPGDTIVGDR
                     PPDVLARRLLEHGGIDGIHINGSVVSVDIAKGHTAEGLKEIIEDLYTYYRPGVEVPQF
                     PAPAE"
     gene            4990..5724
                     /locus_tag="PKY13_05670"
     CDS             4990..5724
                     /locus_tag="PKY13_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79453.1"
                     /translation="MVNPELAEIVERLRPTVLSGERTLPVPEALRPVFPLGGLARGSR
                     LSLRGTGAASICMHTVAEASKAGSWVAVVGVGAWGWAAAARAGWSLERSVFVAEPPTG
                     QWGTVVAALVDAVDLVIVDPVHQVSATDARRLAARSRERGSIVVDLAVDDGRRRFRWP
                     TEADLTLTVESTEWAGLEVGHGVLADRRLRVGADGRRGAARTRRVELSLGSNGALSVI
                     DPAISPAVVGTPAVAAVPSGRHLRAV"
     gene            5714..7336
                     /locus_tag="PKY13_05675"
     CDS             5714..7336
                     /locus_tag="PKY13_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOT79454.1"
                     /translation="MPSEGDAPTRTVVVWCADWPAVALGRPVDRPVVVMFANRVVATN
                     PLARALGVDLGVRRREAQRHCPEAELVERDADREARTFEPVVAALDDITPRIEIVRPG
                     SCLFPARGPSRYFGGDAELADRLTLAVRRQLVIPTAVGVGIADGAFAATLAAKRSAGM
                     EAGGTDVSVIVPPGETPGFLAEFGVGALQQSGPSSPDLVEVLVRLGITTLGSLSALAA
                     SDVVARFGAEGAEAHRLARGLDLRPPRLADPPVDLDVSHEPDPPLERVDQAAFVAKAL
                     ADDLHERLGSRGLAAVAILIEATTTEGHTVERRWRHDGALSSTAVAQRVRWQLDGWLT
                     STQGRRSRGAGALARLRVRPTEVVPDVGRQMGFWGGADAASIRARRGLARVQGVLGSD
                     AVMVAEWRGGRAPGEQYRLVSVDAVAVAADESFDLAAPGPEPWPGRLPDPPPALVWQR
                     PRRAEMHDAEGQMVRVDRRGGCSGEPARLSIESGPWQAVVGWSGPWLSDERWWDALAH
                     RRRARFQLRTVDGTAHLVALESQQWWVEATYD"
     gene            complement(7458..8327)
                     /locus_tag="PKY13_05680"
     CDS             complement(7458..8327)
                     /locus_tag="PKY13_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79455.1"
                     /translation="MTDWFEICRRNARSVQTTIGWIFWDPGAVSRLGALGLPHPLGYI
                     AARAAPLGPAGPEAITAAFGSISPVGIAIATATVAEHTTWDDVWAARDAAVLDGLAEH
                     APAMLEPLAELGPLLWPVVEQLPIAGRTFFAAHLRMERPTDPVLSGWHAVNCVREWRG
                     DTHWALVTAAGLTGTEASVLHNAWLGYERDWLAHSRGSSPEELATAWASLAARGLVDG
                     DPTTGEVNADGIALRQRIEDDTDRLTTLGWELLGEERSRWFAEMFEPPCEQLLARVDI
                     TAGPNYQPASRLR"
     gene            8424..9110
                     /locus_tag="PKY13_05685"
     CDS             8424..9110
                     /locus_tag="PKY13_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT79456.1"
                     /translation="MRVLVIEDEVALADAVARGLEAEGFEVVAVHDGLEGLEKARAER
                     FDLIVLDIMLPGMNGYKVCRTLRDEEVWTPILMLTAKDGEYDEAEALDTGADDFLSKP
                     FSFVVLVARLRALARRRSDANLQPLELGDLVLEPVSRTVRRGDTDIPLSPREFALLEA
                     LMRHTGEVVAKTDLLERVWGEDFEGDPNVVEVYIGYLRRKIDRPFGHSDLETVRGEGY
                     RMGAATHSAS"
     gene            9107..10546
                     /locus_tag="PKY13_05690"
     CDS             9107..10546
                     /locus_tag="PKY13_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT79457.1"
                     /translation="MSSRLAALVGSVRVRLTFAVSIIFAGALTLASFGLVRQVENALV
                     NDVQVRNDTVAQALGKILSAGNPDALSTSDPTQLADGLSGTYDADMLREGLNESIVYA
                     SGPGSATVSQSTNIFDKIRQVITGEAIPLFSKALPSRIDDKDYVVSKVKVPTQQGDMV
                     LSVASSLEPIKRTVDRVQGALFFAVPSLVGAVAILAWMMTGRALRPVSAITSRAQAIT
                     SMTLDQRVPEPRTDDEIGQLARTMNAMLDRLESASERQKRFVSDASHELRSPVASIRL
                     QLETALMRPEQTDWEQVGRTVLAEDQRLAGLVDNLLALARIEEGQRRPQAEVDLDEII
                     HDQLARPRRVSVDRSGVLAGRVYGVRDELTSVVRNLIDNAERHAGSVVKVSLATKGPW
                     VRLAVEDDGPGVEPNLRDKVFERFARLQEARSRDAGGAGLGLALTKRIVETHNGRIFI
                     EDSSLGGAAFVVELPAAELPADDEFEPVD"
     gene            complement(10558..11415)
                     /locus_tag="PKY13_05695"
     CDS             complement(10558..11415)
                     /locus_tag="PKY13_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOT79458.1"
                     /translation="MQSSNPFLNDKYFRGAGAGGAVIDATGTERGWGQVPPSYEAYES
                     TPRTATMTMGGVASATGVMLVFVMVGAWFGWGKVTTEFLGIEQGTGKRVYSADLPMGW
                     LIGSMVIGLVLALICSFKPPMARILGIPYAIAEGVFLGIISHAYDSQSQGIAIQAIVA
                     TAGVFLAMLALYGFRILRVTPRMTKGIIAATFGVLALYAVMFISRLFTDAGTDFMQST
                     SLLSIGVSLLIVGIAAFNLLLDFDFIERGVKEGLPKEMEWFGAFGLIVTLVWLYLELL
                     RLLSKLQRR"
     gene            complement(11508..12704)
                     /locus_tag="PKY13_05700"
     CDS             complement(11508..12704)
                     /locus_tag="PKY13_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT79459.1"
                     /translation="MVRRFTQVTLVVLVTTMLASCGEPGRVTVSGNQAPATGGVSAAP
                     APLSSAAAQYVDDVTAAHHLTAACTVADAVETIASIEEVDVAEDENDIRVVTAYLSRD
                     CSPCLAVGLFDAGPISDDSDPGNAIAGVIVDVIRATLPADLAELATHNDLRVRPTGPA
                     RDYWVANCSDWQTKVLTGLDRARAASTTTRPPDGPNDQSYGDEDSVLGVIGTIRSHPE
                     AGPAPKVLWAVDRGPEAMIVHDGVVYVTDLDAAVTAIDIATGEVAWSVQPDEGADTSG
                     GVTMSYLEGAVRVFIPWSYAAELDAADGSTRSLDSTLGENPWPDQAAWLSDEPGYAFL
                     EGWEPRIAPDDGEKLALVGHTPDGDVSFETTGEPMLDPLPYAFRVGSTVVIADQSGYV
                     VGLDWP"
BASE COUNT         1766 a         4396 c         4675 g         2005 t
ORIGIN      
        1 cgctggtggg ctcggcggcc tgggcgacgt cttgggcggt gcgctcggcg gggcgggcgg
       61 tggaggtgcc ggtggcggca gcctcggtgg ggtgctcggc ggaatgttcg gcggcaaggg
      121 ccgcaagggt ggcgtcgtcg gggtcctcct cgtgttggcc gcggtgttcc tcctcccgaa
      181 gatcatgtcg ggcggcgggt tcgacgtgtc cactgccggt ctcgacaacc tgccgggtat
      241 gtccggcggc ggagcattgc ccggctcggg cgatgtcgcc gacgcgtcgc ttccggccgc
      301 ggacgacccc gatcccgaac tgacccagtt cgccaacgtg ctcatcaccg acaccaacaa
      361 cgtctgggag cagcagttcg acgaggcctc caagcgttac gaccgcacga agctcgtgtt
      421 gttcaccggt ggtgtgaaca ccgcgtgcgg gcaggcgagc tcggacatgg ggccgttcta
      481 ctgtccgggc gaccagctgg tgtacatcga cctgacgttc ttcgaggagc tggcgacgcg
      541 gttcggtgct gcgggtgact tcgcccaggc gtatgtgatc gcgcatgagg tcgggcacca
      601 cgtgcagaac gagctcggta tctcggcgca ggtgcgtcgg ctcgaggagg acgatcccgg
      661 tgccgccaac gggcctgacg gcctctcggt gcggaccgag ctgcaggccg actgtctcgc
      721 cggggtctgg gcccatacgc gctacgagcg cggtgagtcc gatccgacca agcggctcga
      781 cgacggtgac atcgacgagg cgttgcgggc cgccgcagga gttggcgacg actcgatcca
      841 gaagtcggcg acggggcgcg tgaaccccga gggattcaca cacggcacgt cggcgcagcg
      901 gtcccgctgg ttcaagaagg gctacgactc gggcgattcc gagcagtgcg acaccttctc
      961 gaccgactcg ctctgacgtt ctcccggcgc tcctgagccg ctcgacgggg atgcccgcgg
     1021 cgtgagcagt tctgcgggag ttcccaggaa cctctgagcg ccgtccgatg gccggggccg
     1081 atcgttggtt ccatgaccac cgtccacgac cccgagccga ccaccgcggt cacggcgcag
     1141 ccggcggcgg ccccggtcgt tgcgccgacg ctcgacatcg tggtgcccgt ctacaacgaa
     1201 gcccacgtgc tcgacgccaa cgtcgagcga ctcgccgccc acctccgcac caccatgccc
     1261 ttcagctggc ggatcaccat cgtcgacaac ggctcgaccg actcgacgat gcagacggcc
     1321 accgccctgg ccggccggct ggagggggtg cgggtgcggc acctcgatcg caagggccgg
     1381 ggcctcgcgc tccgcaccgc gtgggtgaga gcgactcgcc gatgctcgcc tacatggacg
     1441 tcgacctgtc cacgggactc gatgcgctcc tgccgctcgt tgcgcccctg gtcacgggtc
     1501 actccgacgt ggccatcgga tcacggttgg cgtcgggcgc gtcggtggcg cgcggtccca
     1561 aacgcgagtt catctcccgc tcctacaacc tgctgctgca cacggtgttc gcgacccact
     1621 tccacgacgc ccagtgcggc ttcaaggccc tgcgcgccga tgccgcagcg gccctgttgc
     1681 cccagatcga cgacgacgag tggttcttcg acaccgaact gctgctcgtc gccgagcaca
     1741 acgggctgcg catccacgag gtgcccgtcg actgggtcga cgatcccgac agccgcgtcc
     1801 gggtcgcaca gacggcccgc cgcgacctgg ccggcatggc ccgcatggcg tggcggttcc
     1861 tccgcggcgc cggacggatc gacggcgcca cctacgggcg cgccgagctc gccgaccaga
     1921 tgggtcgcca gctcgtcgtg ttcggactga tcggcacgat cagcaccgga gtctccgcgg
     1981 ggctcttcct ggccctccgc gaaccgctcg gcgcggtgct cgcgaacgtc gtcgccgtga
     2041 cggcgaccgt ggccgtcaac gcctgggcca atcgccggtt cacgttcggg cgccgcgggc
     2101 gtttgggccg ttcgagcgat tacctccgcg ccggggcgat ctacctggtc gggctcgtcg
     2161 tctcgacgtt cgtcctgtcg gcgatcagcc ccaccgatca cctcgtcgag ctcctcgccc
     2221 tgctggcgac gtggtcactc accacggtcg cccgattcac cgtcctcagc cacacgccga
     2281 tcacgtccac ggaggtgcag ccatgacggt tgccgatcac ccggtccccg ccactgcccg
     2341 tgctgcggtg caaccgccga tggagccggc ggctgcagcc caacagcacc ggtcggcgcg
     2401 ccttcgtcgt gtggtccggg ggcgtgacga cgacccggtg tgggcccggc cggccctcgt
     2461 cggactcctg gtcggcaccg ccgtgctgta cctctggacc ctgtcggagt cgggatgggc
     2521 gaacgccttc tactccgcgg ccgtgcaggc cgggtcgcag agctggaagg cgttcttcta
     2581 cggctcgtcc gacgccgcga actccatcac cgtcgacaag cccccgatgt cgttgtggcc
     2641 gatggcgttg tcggtccggg tcctggggct gagctcgttc agcatcctgc tgccacaggc
     2701 gttgatgggc gtcgccaccg tcggtgtgct gtacgccgcg gttcgccgct ggttcagcgc
     2761 ccacgcggcg ttgctggcgg gtgtcgtgat ggcgttgacc ccggtcgcgg cgctgatgtt
     2821 ccgcttcaac aaccccgacg cgctcctgac gctcctcatg gtgatcgccg cctacttcgc
     2881 cctccgggcc atcgagagcg gtgcgtggaa gtggttcgtc gccacgggcg tggccgtcgg
     2941 gttcgggttc ctcaccaagc agctccaggt gctgttgatc gtgccggggc tcggtgcggc
     3001 gcatctcgcg ttcggcggca acgggttctg gcgacgcatc cgtgacctgg cgatcgccgc
     3061 cgcagcgatg gtcgtgtccg ccggctggtg gatcgccatc gtcgagctgg tgccggcgag
     3121 catgcggccc tacgtcggtg gatcccagga caactccatc ctcgagctga ccctcgggta
     3181 caacggtctc ggtcggctca ccggcaacga gaccggcagc gtcggcgggg gcaacgggcc
     3241 cggcggtggc atgtggggcg agacggggat cggtcgactg ttcggcaacg agatcggcgg
     3301 tcagatcacc tggctggtcc cggcggcgct gatcctcctc gccgcagtgc tggtcgtgtg
     3361 gtggcggcga ccacgccgcg acctgcaacg tgcgtcgatc gcggtgtggg gttcgtggct
     3421 cgtcgtcacc ggcgccgtgt tcagcttcat gagcgggatc ttccacgcct actacaccgt
     3481 cgccctggcg ccggcggtcg ctgcgctcgt gggcatcggt gccgtgacgc tctggcagca
     3541 ccgcaccacg tccccccggg cccacgccgc gacgatgacg gcgctggcgg tcatcgccgg
     3601 tgtgacgacc tggtgggcac gggtccttct ggcccgcgcc gcggactgga acccctggat
     3661 ctcgacactc gtcctcgttg ccggaggaac cgccgtcggc ggcctgctcg ccgcggcctg
     3721 gttgcggccc ctctggggtg accgggcggc caactggctc ggagtcggag ccgccggtct
     3781 ggcgctcgtg gcgggactcg ccggcccgac cgcgtggacg ttgcagaccg tcgccactcc
     3841 ccacaccggt tcgatcgtca ccgcgggacc gaccgtcacg ggaggcgggt tcggtcccgg
     3901 ccggggtggc ttccccggcg gcatgaccgg aacgatgccc ggtgggctcc cgggtggttt
     3961 ccccggtggg aacccaccgg ccatcggtac caccccgccc ggtggcacga cgggtggctt
     4021 ccccggcggg ctcggtgccc gccccggcgg tggtggcgga atgggtgggt tgctcgacgc
     4081 cgtgactccc ggcgccgaga tcgtcgcggc gctcagcgag gacgcttcga gctacacgtg
     4141 ggtcgcagca gcggtcggct ccaacaacgc ctcgggatac cagctcgcga ccgaactgcc
     4201 ggtcatggcc atcggtgggt tcaacggctc ggatccgtcg ccgacgctgg ccgagttcca
     4261 gcagtacgtc gccgacggga agatccactg gttcatcggc ggtggcggct tcggcggcgg
     4321 cgggttcgga aaccagatgg gcggcagcga cgcatcgtcg gagatcgcca cgtgggtgca
     4381 ggagaacttc accgccacga ccatcgacgg ctccaccctc tacgacctca ccgccccgat
     4441 cgcctgagcg ccgacccgaa tcctcgacag gtcgggtggc atcggggggt ggttttcggg
     4501 tggagcgtcg ccgcccgcta cgctgcggac gctatgggac agccggtcac cgtcatcgag
     4561 aagcagtcac cgaccacggg tgtggttcgg ttcgagctca accgtgtgct caccggcacc
     4621 ggccacgagc gctacaagcc gggcgacacg atcgtcggcg atcgtccgcc cgacgtgctg
     4681 gcccgtcgcc tgctcgagca cggcggcatc gacggcatcc acatcaacgg ctcggtcgtg
     4741 agcgtcgaca tcgccaaggg ccacaccgcc gagggcctca aggagatcat cgaggatctc
     4801 tacacgtact accgtcccgg cgtcgaggtc ccgcagttcc ccgctccggc ggagtaaccc
     4861 cgtcccgcca gtggttcgcg gtgacgtgtg atcaccgcct ggtgaccact caccgcagat
     4921 ccggtggtgg tcctcgacgc gcccggacgg cccttgcaca gtatcgaaca ggtgttcgat
     4981 actgtgcgga tggtgaaccc ggagctggcc gagatcgtcg aacgtctccg tccgacagtc
     5041 ctgtcggggg agcggacgct gccggtaccg gaggcgttgc ggccggtgtt ccccctcggg
     5101 ggcctggccc gtgggtcccg cctgtcgttg cgggggacgg gggctgcgag catctgcatg
     5161 cacacggttg ccgaggcgtc gaaggcgggt tcgtgggtgg cggtcgtcgg cgtgggggcg
     5221 tggggatggg cggcagctgc ccgggccggc tggtcgctcg agcggagtgt gttcgttgcc
     5281 gagccgccca ccggtcagtg gggcacggtc gtcgcggcgc tggtcgacgc ggtcgacctc
     5341 gtcatcgtcg atcccgtcca ccaggtcagc gccactgatg cgcggcgcct ggcggcgcga
     5401 tcgcgcgaac gggggtcgat cgtcgttgat ctcgcggtcg acgacggtcg ccgccggttc
     5461 cgttggccca ccgaagccga tctgacactg accgtcgagt cgaccgagtg ggccgggctc
     5521 gaggtgggcc acggggtgtt ggccgatcgt cggctccgcg tcggtgccga cggtcgtcgg
     5581 ggtgccgccc ggacgcgtcg ggtggagctg tcgttggggt cgaacggtgc gctgtcggtg
     5641 atcgaccccg cgatcagccc cgcggtggtc gggacgccgg cggtcgctgc cgtgccctcg
     5701 ggtcgtcatc tccgtgccgt ctgaggggga cgcgccgacc cgcaccgtcg tggtgtggtg
     5761 cgctgactgg ccggcggtgg ccctcggtcg cccggtcgac cggccggtgg tggtcatgtt
     5821 cgccaaccgg gtcgtggcca ccaacccgtt ggcccgggcg ttgggcgtcg acctcggtgt
     5881 ccgccgccgt gaggcgcagc gtcactgtcc cgaggccgag ctcgtcgaac gtgacgccga
     5941 ccgcgaggcc cgtacgttcg aaccggtggt cgccgcgctc gacgacatca cgccgcgcat
     6001 cgagatcgtc cggcccggct cgtgcctgtt cccggcacgg ggtccgtcgc gatacttcgg
     6061 cggcgacgcc gagctcgccg atcgcctcac gctggcggtg cgtcgtcagc tcgtgatccc
     6121 gacggcggtg ggtgtcggga tcgccgacgg tgcgttcgcc gccaccttgg ccgccaagcg
     6181 cagcgccggc atggaggcgg gcggcaccga cgtgtcggtg atcgtgccgc cgggggagac
     6241 gcccggcttc ctcgccgagt tcggggtggg cgccctgcag cagtcgggcc cgtcgtcgcc
     6301 ggacctggtc gaggtgctgg tgcgcctggg gatcacgacg ttggggtccc tgtccgcctt
     6361 ggccgcgtcg gacgtggtgg cacgattcgg cgccgagggc gccgaggccc accgactcgc
     6421 ccgtggcctc gatctccggc cgccgcggct cgccgacccg cccgtcgatc tcgacgtgtc
     6481 ccacgagccc gacccgcccc tcgaacgggt cgaccaggcg gcgttcgttg ccaaggcgtt
     6541 ggccgacgac ctccacgagc gcctgggctc gcgtgggctc gcggcggtgg cgatcctcat
     6601 cgaggcgacc acgaccgagg ggcacaccgt cgagcggcgg tggcgtcacg acggtgccct
     6661 gtcgtcgacc gcggtggcgc agcgggtgcg gtggcaactc gacgggtggc tcacctccac
     6721 ccagggtcgc cgttcccgcg gcgccggggc gttggcgcgc ctgcgggtgc gccctaccga
     6781 agtggtgccc gatgtcgggc ggcagatggg gttctggggt ggtgccgacg ccgcgtcgat
     6841 ccgggcacgc cgggggctgg cccgggtgca gggggtgttg ggttccgacg cggtgatggt
     6901 ggccgagtgg cgcggtggtc gggcgccggg ggagcagtac cgcctggtgt cggtcgatgc
     6961 ggtggcggtg gccgccgatg agtcgttcga cctcgccgcg cccggacccg agccctggcc
     7021 gggtcggctt cccgacccgc cgccggcgct cgtgtggcag cgcccccgcc gcgccgagat
     7081 gcacgacgcc gaggggcaga tggtgcgcgt cgaccgtcgg ggtgggtgct cgggcgaacc
     7141 ggcacggctg tcgatcgagt cggggccgtg gcaggcggtg gtggggtggt cggggccctg
     7201 gttgagcgac gaacgctggt gggacgcgtt ggctcaccgc cggcgtgccc gcttccagct
     7261 gcgcaccgtc gacggcaccg cccacctggt cgcactggag tcccagcagt ggtgggtgga
     7321 ggccacctac gactgacccg gcgcaccggt tcccgcccgt tcccacccgt tcccacccgt
     7381 tcccacctca tttgtcctgc gttttcgccc ctggaggggc gaaaacgcag gacaaatgag
     7441 gtgagaacgg cctgggttca gcggaggcgg gatgccggct ggtagttggg gccagcggtg
     7501 atgtcgacgc gggcgaggag ctgctcgcac ggtggttcga acatctcggc gaaccagcgc
     7561 gagcgctcct cgcccaggag ctcccacccg agggtcgtga gccggtcggt gtcgtcctcg
     7621 atgcgttgtc gcagtgcgat gccgtcggcg ttcacctcgc ccgtggtcgg gtcgccgtcg
     7681 acgagtcccc gcgccgccag cgacgcccac gccgtggcga gctcctccgg cgagctgccc
     7741 cgtgagtggg ccagccagtc ccgctcgtaa cccagccagg cattgtggag caccgacgcc
     7801 tccgtacccg tgagcccagc agcggtgacc agcgcccagt gggtgtcgcc gcgccattcc
     7861 cgcacgcagt tcacggcgtg ccaccccgac agcaccggat cggtgggccg ctccatgcgc
     7921 aggtgggcgg cgaagaacgt acgaccggcg atcggcagct gctcgacgac gggccacagc
     7981 agtgggccga gctcggccaa cggctcgagc atcgccgggg cgtgctcggc gaggccgtcg
     8041 agcacggcgg cgtcacgggc cgcccacacg tcgtcccacg tcgtgtgctc ggcgacggtc
     8101 gccgtggcga tcgcgatgcc gacggggctg atcgagccga acgccgcggt gatcgcctcg
     8161 ggaccggccg ggccgagcgg tgcggcccgg gcggcgatgt agccgagggg gtgggggagc
     8221 ccgagcgctc cgagccgcga caccgccccc gggtcccaga agatccagcc gatggtcgtc
     8281 tggaccgatc gggcgttgcg gcggcagatc tcgaaccagt cggtcatggc ggcgaggata
     8341 ggccgggaac ccctgtgacc tcccacagcg acagcgcacc gccatgggcg gcatccggtg
     8401 ccgccacccg gcagaatgga cccgtgcgag tcctggtgat cgaagacgaa gttgcgttgg
     8461 ccgatgccgt cgcccgcggc ctggaggccg aggggttcga ggtcgtggcc gtccacgacg
     8521 ggctcgaggg cctcgagaag gcccgcgccg agcggttcga cctgatcgtc ctcgacatca
     8581 tgttgcccgg catgaacggc tacaaggtgt gccgcaccct ccgcgacgag gaggtgtgga
     8641 ccccgatcct catgctcacg gcgaaggacg gcgagtacga cgaggccgag gcgctcgaca
     8701 ccggcgccga cgacttcctg tccaagccgt tctcgttcgt ggtgctcgtc gcccgcctcc
     8761 gggcgctggc gcggcgccgc agcgacgcca atctccagcc gctcgaactc ggcgacctcg
     8821 tgctcgagcc ggtgtcgcgc acggtgcgcc gcggcgacac cgacattccc ctgtcgccac
     8881 gggagttcgc cctgctggaa gcgctcatgc gccacaccgg tgaggtcgtc gccaagaccg
     8941 acctgctcga acgggtgtgg ggcgaggact tcgagggcga tcccaacgtc gtcgaggtgt
     9001 acatcggcta tctccgccgc aagatcgacc gcccgttcgg ccacagcgac ctcgagaccg
     9061 tccggggcga gggctaccgc atgggggcag cgacccacag cgcgtcatga gttctcggct
     9121 ggccgccctc gtcggctcgg ttcgggtccg cctgacgttc gccgtctcga tcatcttcgc
     9181 cggcgccctc acgttggcgt ccttcgggct cgtccgtcag gtcgagaacg cgctcgtcaa
     9241 cgacgtgcag gtccgcaatg acaccgttgc ccaggcgctt ggcaagatcc tgtcggccgg
     9301 caaccccgac gccctgtcca cgtccgaccc gacccagctc gccgacgggc tgagcggcac
     9361 ctacgacgcc gacatgctcc gcgagggcct caacgagagc atcgtgtacg cgagcggccc
     9421 gggttcggcg acggtctcgc agtcgacgaa catcttcgac aagatccgcc aggtcatcac
     9481 cggcgaggcg attccgctgt tcagcaaggc gttgccgtcg cggatcgacg acaaggacta
     9541 cgtcgtcagc aaggtgaagg tgccgaccca gcagggcgac atggtgttga gcgtcgccag
     9601 ctcgctcgaa ccgatcaagc gcaccgtcga ccgggtgcaa ggggcgttgt tcttcgcggt
     9661 gccgtcgctc gtgggcgcgg tcgcgatcct ggcgtggatg atgacgggcc gtgccctccg
     9721 gccggtgtcc gcgatcacgt cgcgggcaca ggcgatcacg tcgatgaccc tcgaccagcg
     9781 ggtgcccgag ccccgcaccg acgacgagat cggccagctg gcccgcacga tgaacgccat
     9841 gctcgaccgc ctcgagtcgg cgtcggagcg ccagaagcgg ttcgtgtccg acgcgagcca
     9901 cgagttgcgg tcgccggtcg cctcgatccg cctgcagctg gagacggcgt tgatgcgccc
     9961 cgagcagacg gactgggagc aggtcggtcg caccgtcctg gccgaggacc agcgcctggc
    10021 cgggctggtc gacaacctgc tcgcgctcgc ccggatcgag gagggccagc gccggccgca
    10081 ggcggaggtc gacctcgacg agatcatcca cgaccagctg gcccgccctc gtcgcgtcag
    10141 cgtcgatcgg tccggcgtcc tcgccggccg ggtctacggc gtgcgcgacg agctcacgag
    10201 cgtggtccgg aacctgatcg acaacgccga acgccacgcc ggctcggtgg tgaaggtgtc
    10261 actggcgacg aagggcccgt gggtccgtct cgccgtcgag gacgacggcc ccggggtcga
    10321 gcccaacctg cgcgacaagg tgttcgagcg attcgcccgc ctgcaggagg cccggagccg
    10381 cgatgccggc ggggccgggt tgggcctggc gctcacgaag cgcatcgtcg agacccacaa
    10441 cggtcggatc ttcatcgagg actcgtccct cggtggggca gcgttcgtgg tggagctgcc
    10501 ggccgccgag ctccccgccg acgacgagtt cgagccggtc gactgattgc gtatcgctca
    10561 gcggcgctgc agcttcgaca gcaggcgcag cagctcgagg tagagccaca cgagggtgac
    10621 gatcaggccg aaggcgccga accactccat ctccttcggc agaccttcct tgacaccgcg
    10681 ctcgatgaag tcgaagtcga ggagcaggtt gaaggcggcg atgccgacga tcaggaggct
    10741 gacgccgatg ctcaggaggc tggtcgactg catgaagtcg gtgccggcgt cggtgaacag
    10801 gcgggagatg aacatgaccg cgtagagcgc cagcacgccg aaggtggcgg cgatgatgcc
    10861 cttggtcata cggggggtga cccgcaggat gcggaatccg tagagggcga gcatggcgag
    10921 gaacaccccg gcggtggcga cgatcgcctg gatcgcgatg ccctggctct gggagtcgta
    10981 ggcgtgggag atgatgccga ggaacactcc ctcggcgatc gcgtagggaa tgccgaggat
    11041 gcgggccatc ggcggcttga acgaacagat cagggcgagc acgaggccga tcaccatcga
    11101 accgatgagc caacccatgg ggaggtcggc gctgtagacc cgtttgccgg tgccctgctc
    11161 gatgccgagg aactcggtgg tgaccttgcc ccacccgaac caggcgccga ccatcacgaa
    11221 gacgagcatc acgccggtgg ccgatgcgac cccgcccatc gtcatggtgg cggtccgcgg
    11281 cgtggactcg taggcctcgt agctcggggg gacctggccc cacccgcgtt cggtgccggt
    11341 ggcgtcgatg acggcgccgc ccgcgccggc gccacggaag tacttgtcgt tgaggaaggg
    11401 gttgctcgac tgcatctggg cctcctgggc cgtcccggct ctgcgcgggg attcgtcggt
    11461 cagcgtaccg ctggggcgag ggagcgacgg tgcaggtcgg cgtgggctca gggccagtcg
    11521 agaccgacga cgtagccgga ctggtcggcg atgacgaccg tcgagccgac gcggaacgcg
    11581 tagggaaggg ggtcgagcat cggttcgccg gtcgtctcga acgacacgtc gccatcgggc
    11641 gtgtggccga cgagggcgag tttctcaccg tcatcggggg caatgcgggg ctcccacccc
    11701 tccaagaacg cgtagccggg ttcgtcgctc aaccacgcag cctggtcggg ccacgggttc
    11761 tcgccgagcg tgctgtcgag cgagcgggtg gagccgtcgg ccgcgtccag ctcggcggcg
    11821 tagctccacg ggatgaacac ccgcaccgca ccctcgaggt acgacatggt cacaccgccc
    11881 gaggtgtcgg cgccctcgtc gggctggacc gaccacgcca cctctccggt ggcgatgtcg
    11941 atggccgtca ccgctgcgtc gaggtcggtc acgtagacga cgccgtcgtg gacgatcatg
    12001 gcctcgggac cccggtcgac ggcccacagg accttcgggg ctgggccggc ctcggggtgc
    12061 gaacggatcg tgccgatcac gccgagcacg ctgtcctcgt cgccgtagga ctgatcgttc
    12121 gggccgtcag ggggccgagt ggtggtcgag gcggcccggg ctcggtcgag cccggtcagt
    12181 accttggtct gccaatcgga acagttggcc acccagtaat cgcgtgccgg ccccgtcggc
    12241 cgcacgcgga gatcgttgtg ggtcgcgagc tcggcgagat ctgcaggcag ggtggcacgg
    12301 atgacgtcga cgatcacgcc ggcaatcgcg ttcccgggat cggagtcgtc ggagatcggc
    12361 ccggcgtcga acaagcccac ggcgaggcag ggagagcagt ctcggctcag ataggcggtg
    12421 accacacgga tgtcgttctc gtcctcggcg acgtcgacct cctcgatcga cgcgatcgtc
    12481 tccaccgcgt cggcgacggt gcacgcagct gtgaggtggt gtgctgccgt gacgtcgtcg
    12541 acgtactgcg ccgcggccga ggagaggggc gcgggggccg ccgagacccc gccggtggcc
    12601 ggcgcctgat tgccggagac ggtgacgcgt ccaggctccc cgcacgatgc gagcatcgtt
    12661 gtcacgagga cgacgagcgt gacctgggtg aaccggcgga ccatgtggcg aggataacgg
    12721 agttcaccct caaggaattc accgcagtcc gccgatgaag ggcggggcgg gcggtcggaa
    12781 cgatgacgcg gtccgtcgag gagcgatccg cgtgcgtcat ggacgaccaa tcagccgaca
    12841 gc
//
LOCUS       DAOSOA010000059        12526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5020_length_12526_cov_6.325235, whole genome shotgun sequence.
ACCESSION   DAOSOA010000059 DAOSOA010000000
VERSION     DAOSOA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5020_length_12526_cov_6.325235"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PKY13_05705"
     CDS             complement(<1..891)
                     /locus_tag="PKY13_05705"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOT79460.1"
                     /translation="MDGTPDRAMTWINGAIVAVAVLTLVGLVAFWPRGDAPDLGLGHL
                     RYVDATITSVTTSQCRSIEVPDAFSGCREVGVRLTSGDRDGEPATFTVYDTQFEVPDL
                     HEDDDVVLLDSPSSPDEVRYAFSDFQRGTPMVLLALGFGVVVIAFGRRRGLRALFGLA
                     ASALILVVFIVPALLRGSAALPVALVGTAAVAYLALYLAHGITPPTTVALAGTLASLV
                     VIAMLSVLVGAVAHLSGLSDEAAQTIRVTAGSIDLRGLLIAGFVLGALGVLDDVTVTQ
                     VSTVQALLRANPSMPRRDLYR"
     gene            complement(929..2446)
                     /locus_tag="PKY13_05710"
     CDS             complement(929..2446)
                     /locus_tag="PKY13_05710"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOT79461.1"
                     /translation="MLLLADLVTTSGSVAATSSRRAKTTALAELLVRLEQHEVVPAVS
                     YLIGVIPQGRIGVGWATSAIDGTAERPSVTVSDLDADLAALSVIGGPGSQAARRAIID
                     DLGRRLTPPEAGFVRRLLVGELRQGALAGVMTEAIAAASAIPASAVRRAAMLSGDLAG
                     SARLALVEGATAIDAVGLTVGVGVEPMLASTAADVTEALAGTGPASVEWKLDGVRIQA
                     HRDGDEVRLYTRNLNDVTDRLGAVGDLLSALPVRSIVLDGELLGISEDGTPEAFQDSA
                     SSFGRRSDGAAAGLSIAFFDIVHLDGNDLLDLPLGERLDILASVDGLPRIPSTRTDDP
                     QVAQQVLDDALAQGHEGVMVKDLSSPYAAGRRGAAWRKIKPVHTLDLVVLAVEWGSGR
                     RRGKLSNLHLGALDDDGTPVMVGKTFKGLTDELLAWQTERFLAIATEQDDHVVHVRPE
                     QVVEIALDAAQASTRYPGGVALRFARVRRYRDDKSAADADTLDAVRALLPRSRRG"
     gene            2501..3319
                     /gene="nudC"
                     /locus_tag="PKY13_05715"
     CDS             2501..3319
                     /gene="nudC"
                     /locus_tag="PKY13_05715"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOT79462.1"
                     /translation="MSSFSPSFPPTFPPPFLPAVVPGADVTAHRYVHVIGASVHFAEE
                     NDLGDEHAHFIGVLDGVGLWAVDVPAGQDPSDGAALDLFTLSVRAPEAEWVAAGRAVQ
                     IAEWARTHRFCGRCGSRTEPVPNERAMRCPECRLLAFPRVAPAMITLVTKGDPGPDQQ
                     VLLAHGVQFPMPMYSCLAGFVEPGETLEGAVVREVMEEVGVLVGNVTYRSSQPWPFPH
                     SLMIGFRADWQAGDILCDPTEIVDAGWYTRDALPNTPPGVSIARRLIDEWIAEV"
     gene            complement(3377..4171)
                     /locus_tag="PKY13_05720"
     CDS             complement(3377..4171)
                     /locus_tag="PKY13_05720"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOT79463.1"
                     /translation="MPIHSDLTTDLLLVDIDARVATVTLHRPGQRNALSRALQRDLRA
                     AVTAIEADDGIDVVVLTGSDPAFCAGLDLKELGSGELLTGDDSTLSGRGPLPARSKPW
                     IGAVNGAAVTGGFELALACDFLVASERASFADTHARVGVMPGWGLTALLPEAIGVRRA
                     REMSVTGNYLDARTAAEWGLVNHVVSHDELLPFCHGLAADIVSNDRDGVRQMLDTYQQ
                     VVGRTPDDARSVEATISARWMRERHDPAAVEERRRGIVERGRSQQG"
     gene            complement(4194..4466)
                     /locus_tag="PKY13_05725"
     CDS             complement(4194..4466)
                     /locus_tag="PKY13_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOT79464.1"
                     /translation="MLWILGAIVVGLISGFIARAIVPGDDSMGIGGTIVLGLVGSLVG
                     GFIGYLIFHKDSTNGAFQMSGFLGSIVGSVLVLLAWRAYNSRTAVH"
     gene            complement(4586..5746)
                     /locus_tag="PKY13_05730"
     CDS             complement(4586..5746)
                     /locus_tag="PKY13_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOT79465.1"
                     /translation="MIPTAVVGVGQTHHKSRRRDVSFGGLVREAVFRALDDAEMTMSD
                     IDAVVLGKAPDLFEGVMKPELYLSDALGAVGKPMFRVHTAGSVGGTTAIVAASHVQTG
                     KHRTVLAVAFEKQSEGNAQFALGSGKGASLGAGGAFAPFIRSYIHRSGAPEHIGWKVA
                     VKDRLNALKNPYAHLKIEDISIEKVKASPMMWDPIRFLESCPSSDGACAVVLTDEDGG
                     KAAAAAGRPPAWILGASSRSEPPAFPGRDPVRPAACVQCADDVYAQAGITDPRRQLDM
                     AELYVPFSWHEAIWLEAHGIAEPGDGWKMIDDGSTELGGSFPVNCSGGVLSSNPIGAS
                     GLLRFAEAANQVRGAAGEHQVENARTALAMAYGANSQYFSTWVVGSSLQPFD"
     gene            complement(5743..6804)
                     /locus_tag="PKY13_05735"
     CDS             complement(5743..6804)
                     /locus_tag="PKY13_05735"
                     /inference="COORDINATES: protein motif:HMM:NF005924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HOT79466.1"
                     /translation="MVSATSTDIAVVGWAQSPMLRHTKQSETQMLMDVITAALDPLGL
                     TRADVDFTCLGSCDYITGQAFSFVSNLDAIGAWPPKRDSHVEMDGAWALYEAWLRLQE
                     GDIDIAVVTGSGRSSTGDPSLIYPMEMDPYFLAPLGADPLTLAALQARAVIAAGLADE
                     RSMAEVAMRSRGPEGNGGKTIDTLLGEEYVREPLRRHDVPPITDGAAAMVLARGDRAR
                     ELVERPAYITGFDHRTECHNPSFRALDDAPSVRIAADAAGLGSGPVELAELQAAFTHE
                     ELLLRTVLGLGDEVTVNASGGALKANPIMATGLCRIGHAADHIFGGGHRALAHSTSGP
                     CLQQNLICILEDSNEGGHS"
     gene            complement(6804..7796)
                     /locus_tag="PKY13_05740"
     CDS             complement(6804..7796)
                     /locus_tag="PKY13_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOT79467.1"
                     /translation="MTRFVESTITFPYRRSLGPVIGTFMSALTERRILGIRNGDAVLV
                     PPMEWDPATGAELAHDFIEVGPAGTVESWTWVPHPSEQHPLGHAFSFAFVRLDGATTP
                     LLHAVDAGSPEAMSAGMRVAPRWKATRVGRLDDIACFVPGETPEIEGGTDLTETAGEP
                     VEMMDYVASITYRNPVPAATVRAVAASREARLLGSKCPVCGRIYSGGRGYCPIDAVEI
                     GPADEVDLPQSGTITNFAIVTPVQYPGQTETEPFVRAFVLLDDTDVILSYQPVIELPY
                     DEVHVGQRVTAVWASPAEETEDGGAMGGAYGSLLGWIPSGEPSIDDPDLVNRIF"
     assembly_gap    7846..7945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8598..8993)
                     /locus_tag="PKY13_05745"
     CDS             complement(8598..8993)
                     /locus_tag="PKY13_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT79468.1"
                     /translation="MYVRTKPTRCVASSPAMARAWVRESLEDWDGDPAVDHAELLVSE
                     VATNALLHGGTDRPVVDVTHSAELVRVSVRDRTRDRPARTFKDDPMTPGGYGVVLLDE
                     ISSRWGVAQRVGGKTVWFELTSTAGHRRT"
     gene            9309..10484
                     /locus_tag="PKY13_05750"
     CDS             9309..10484
                     /locus_tag="PKY13_05750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT79469.1"
                     /translation="MPVITATTAPNWCRGDIEERAWIVGAEVAARFADEVDRDARFPV
                     EAVEAMRDAGLLSAMVPIELGGAGASLGTIATATRALAYHCSASALVMAMHQIEIWYL
                     NQFGNTDGLRGLLRSVATDGWLIANANSEVGLGGEVGRSFCAVEHGEGTFHLEKEALA
                     ISYGAHADALLLIARRSPEAPENEQVMVAARPGRFSLEQTSSWDTMGLRGTCSSSFKI
                     VVDEAADMVFPTPWLTIGSRCVGATTILLNSTWLGIAEAAAARAHAFVRADARRKIGT
                     LPAAAPLLGEVSVKLGEARAVMDSSVRAYEAAVGTEDIDATSLLLTVRNLKLSAADLA
                     IEIVLKASRICGLAGYKRDTPYAMDRNLRDVIGGPLMANNLRAAGDNAQLLLGMKQF"
     gene            complement(10497..11840)
                     /locus_tag="PKY13_05755"
     CDS             complement(10497..11840)
                     /locus_tag="PKY13_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT79470.1"
                     /translation="MTVPAESEPTESKPTDAADRPVDLPYLGVTYDLTSSSPMELLTR
                     LGDLCRIVWIVDAGDEALGPMRRLLTRAGTVFDTEGRPPAEVAAALVDARFEGHPLAG
                     VIAFTDSQLVLAATIADVLGLPGNPMSAVTRLNDKYHQRQAMLDAGLAVPAFVRIPTG
                     SDPAAGAALASGLRTPLVLKPLRGDSSRDVVAVADRAELQAALTVVATRDVTGDLIVE
                     EYLADREPAANGRIGGYVSVESLVVDGEVIPVAITGKFPLAHPFRETGNFMPHPLDAA
                     GASEVIDLSGAAARSLGVRSGALHTEIKLTPDGPRIIEVNGRIGGGAIDALYARRFGT
                     SLTELAAAIALGIAPDAIAELPASADGPFLYEFFAQPPTTATALRAIGGLDAIVGVAG
                     AESVSPNRSTGDALDWRNGSQGYVLRVSGTADDLAVLGSVPTAVLTAALIEYDED"
     gene            12067..>12526
                     /locus_tag="PKY13_05760"
     CDS             12067..>12526
                     /locus_tag="PKY13_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79471.1"
                     /translation="MACSVDNFWLPTGDGATGACWFWPEGAVASTGVLVVPGIIHEQQ
                     TMAVGLVALAKELASNGVPTLMIDLAGTAQGTGRLDSPRTGQRWDDEVRSAVHHMRDA
                     GLQHIVVLGVRLGALIAAHATVDEPVDLMVMWAPVLSGRRYVRELQLMQAS"
BASE COUNT         1849 a         4219 c         4527 g         1831 t
ORIGIN      
        1 gcggtagagg tctcggcggg gcatcgacgg gttcgcccgc agcagcgcct gcaccgtcga
       61 cacctgggtc acggtgacgt cgtcgagcac cccgagcgct cccagcacga atcccgcgat
      121 caggaggccg cggaggtcga tcgacccggc cgtcacccgg atggtctggg ccgcttcgtc
      181 ggagagcccc gacaggtgcg cgaccgcgcc gaccagcacc gagagcatcg cgatgacgac
      241 gaggctcgcg agcgtgccgg cgagcgccac ggtggtcggc ggggtgatcc cgtgcgccag
      301 gtagagcgcg aggtaggcaa cggccgcggt gccgacgagg gcgacaggga gcgcagccga
      361 accccgcagg agcgccggaa cgatgaacac gacgaggatc agcgcgctgg cggcgagccc
      421 gaacagggct cggaggccac ggcgtcgacc gaaggcgatc accacgacgc cgaaccccag
      481 ggccaggagc accatcggcg tcccgcgttg gaagtcggag aacgcgtacc gcacctcgtc
      541 ggggctcgac ggcgagtcca ggagcaccac gtcgtcgtcc tcgtggaggt cgggcacctc
      601 gaactgggtg tcgtagacgg tgaacgtcgc cggctcaccg tcgcggtcac ccgacgtgag
      661 ccgcacgccg acctcacgac accccgagaa ggcgtcgggc acctcgatcg accggcactg
      721 cgacgtcgtg accgacgtga tcgtggcgtc gacgtagcgg aggtggccca agccgagatc
      781 gggagcgtca ccgcggggcc agaaggcgac cagcccgacg agggtcagga cggcgacggc
      841 gacgatcgct ccgttgatcc acgtcatggc ccgatctggt gtgccgtcca cggcccggga
      901 ccctaccgtc atcgtccgtc gggggcgctc agccccggcg cgagcgggga agcagggcgc
      961 ggacggcgtc gagcgtgtcg gcgtcggcgg ccgacttgtc gtcgcggtag cggcggaccc
     1021 gggcgaagcg gagcgcgacg ccgcccgggt acctggtcga cgcctgcgca gcgtcgagcg
     1081 cgatctcgac cacctgctcg ggacggacgt ggacgacgtg gtcgtcctgc tcggtggcga
     1141 tggcgaggaa ccgttcggtc tgccaggcca ggagctcgtc ggtgaggccc ttgaacgtct
     1201 tgccgaccat gaccggggtt ccgtcgtcgt cgagcgcgcc gaggtggagg ttcgagagct
     1261 tgccgcgacg gcggcccgat ccccactcga cggcgaggac gaccaggtcg agggtgtgga
     1321 ccggcttgat cttgcgccac gccgcgccgc gccgaccggc ggcgtagggc gacgagaggt
     1381 ccttcaccat gacgccttcg tgaccctgcg ccaacgcgtc gtcgagcacc tgctgcgcga
     1441 cctgtgggtc gtcggtgcgc gtcgacggga tgcgggggag gccatcgacg gaggcgagga
     1501 tgtcgagtcg ctcgccgagg ggcaggtcga ggagatcgtt cccgtcgagg tggacgatgt
     1561 cgaagaaggc gatgctcagg ccagcagcgg cgccgtcgct ccggcggccg aagctggacg
     1621 ccgagtcctg gaaggcctcg ggcgtgccgt cctcgctgat gccgaggagc tcgccgtcga
     1681 gcacgatcga tcgcaccggg agcgccgaca gcaggtcgcc cacggcgccc agtcgatcgg
     1741 tcacgtcgtt gaggttgcgg gtgtagaggc gaacctcgtc gccgtcgcgg tgcgcctgga
     1801 tgcggacgcc gtcgagcttc cactcgaccg acgcgggccc ggtgccggcg agggcctcgg
     1861 tgacgtcggc cgccgtggag gccagcatgg gctcgacccc gacgccgacg gtgagcccga
     1921 cggcgtcgat ggcggtggcg ccctccacga gcgccagccg ggcgctcccg gcgaggtcgc
     1981 cgctcaacat ggcggcacgg cggaccgccg acgccgggat cgccgatgcg gcggcgatcg
     2041 cctcggtcat cacacccgcc agcgctcctt ggcgcagctc gccgacgagg agccggcgca
     2101 cgaagcccgc ttcgggcggc gtcaggcgac ggcccaggtc gtcgatgatc gcccggcgcg
     2161 ccgcctggga gcccggcccg ccgatgaccg agagcgcagc gaggtcggcg tcgaggtccg
     2221 acacggtcac cgacggccgt tccgccgtcc cgtcgatggc cgacgtcgcc caccccaccc
     2281 cgatacggcc ctgggggatg accccgatca gatacgacac cgccggcacg acctcgtgct
     2341 gctcgagccg gacgagcagc tcggccaggg ccgtggtctt ggcgcgtcgc gagctcgtcg
     2401 ccgcaacgga gccggaggtc gtgacgaggt cggcgagcag cagcacccca cgattgttgc
     2461 agtgccagcg agtcccggtc cgtcgacgta atgtcccccc atgtcgtcct tctcgccgtc
     2521 gttccctccg accttcccac cgccgttcct ccctgcggtg gtgccgggcg ccgatgtgac
     2581 ggcacaccgc tacgtgcacg tgatcggagc gtcggtccac ttcgccgagg agaacgatct
     2641 cggcgacgaa cacgcccatt tcatcggcgt gctcgacggt gtcggactgt gggccgtcga
     2701 cgtgcccgcg ggccaggacc cgtcggacgg cgcggcgctc gacctgttca cgttgtcggt
     2761 gcgggcaccc gaagccgaat gggtcgctgc gggccgggcg gtccagatcg cggagtgggc
     2821 ccgcacccac cgcttctgcg gtcggtgtgg ttcccgcacc gagccggtgc cgaacgaacg
     2881 ggcgatgcga tgcccggagt gccgcctcct ggcgttcccg cgagtggccc cggcgatgat
     2941 cacgctggtc acgaagggag atcccggtcc ggaccagcag gtgctgttgg cccacggagt
     3001 ccagttcccg atgccgatgt actcctgcct cgccgggttc gtcgagccgg gcgagacgct
     3061 cgaaggcgcc gtcgtccgtg aggtgatgga agaggtcggc gtcctggtcg gcaacgtcac
     3121 ctatcgcagc agccagccct ggccgttccc tcacagcctg atgatcggct tccgagccga
     3181 ttggcaggcc ggcgacatcc tctgcgaccc gaccgagatc gtcgacgccg gctggtacac
     3241 ccgcgacgcg ctgccgaaca cgccgcccgg cgtgtcgatc gctcggcgcc tcatcgacga
     3301 atggatcgcc gaggtctgac cctccgtccg cgaccggagc aacgacgccc gagcgatgag
     3361 cccccgactc ggcgggtcac ccctgctggg accgaccccg ttcgacgatg ccgcggcgac
     3421 gttcctccac cgctgcgggg tcgtggcgct cgcgcatcca ccgtgcggag atcgtggcct
     3481 cgacggagcg ggcgtcgtcg ggggtcctgc cgacgacctg ctggtaggtg tcgagcatct
     3541 gccggacccc gtcgcgatcg ttcgacacga tgtcggcggc gagaccgtgg cagaacggca
     3601 gcagctcgtc gtgggagacg acgtggttga cgaggcccca ctcggcggcg gtgcgggcat
     3661 cgaggtagtt gccggtcacg ctcatttccc gggcgcggcg cacgccgatc gcctcgggaa
     3721 gcagcgcagt gaggccccaa cccggcatga cgccgactcg ggcgtgggtg tcggcgaacg
     3781 acgcccgttc gctggccacg aggaaatcgc atgccaacgc gagttcgaag ccgccggtga
     3841 cggcggcacc gttgacggcg ccgatccacg gcttggagcg ggccggcagc gggccgcggc
     3901 ccgacagcgt cgaatcgtca cccgtcagca gctcgcccga tcccagctcc ttgaggtcga
     3961 ggccggcgca gaacgcgggg tcgctcccgg tgagcaccac gacgtcgatg ccgtcgtcgg
     4021 cctcgatcgc cgtgacggcg gcgcggaggt cgcgctgcaa ggcccggctc aacgcgttgc
     4081 gttggccggg ccggtgaagg gtgacggtgg caacccgtgc gtcgatgtcg acgaggagga
     4141 ggtcggtcgt caggtcgctg tggatcggca tgcaccgatc ctgtcaggtc gggtcagtgg
     4201 accgcggtgc gactgttgta cgcgcgccag gcgagcagca cgagcacgga accgacgatg
     4261 ctgccgagga atcccgacat ctggaaggcc ccgttggtgc tgtccttgtg gaagatcagg
     4321 tagccgatga atccgccgac gagggagccg acgaggccca ggacgatcgt gccgccgatg
     4381 cccatcgagt cgtcgcccgg gacgatggcg cgagcgatga accccgagat gagtccgacg
     4441 acgatggctc cgagaatcca caacatggct gttccttccg tacttcgctg gacgaaggtt
     4501 cgttgagtac tgcaggccgc ggtggcctga gcgtccggta cccgccggcc gaccgcggca
     4561 cacccccgaa ccggggggtc cgggttcagt cgaacggctg cagcgagctg cccaccaccc
     4621 acgtgctgaa gtactgactg ttggcgccgt atgccatcgc gagcgccgtc cgggcgttct
     4681 ccacctggtg ctcgccggcg gcgccccgca cctggttggc cgcctcggcg aagcgcaaca
     4741 accccgatgc tccgatcggg ttcgacgaca ggacccctcc cgagcagttc acggggaacg
     4801 atccgccgag ctcggtggag ccgtcgtcga tcatcttcca gccgtcgccg ggctcggcga
     4861 tgccgtgggc ttcgagccag atggcctcgt gccacgagaa cggcacgtac agctcggcca
     4921 tgtcgagctg tcggcggggg tcggtgatac cggcctgggc gtacacgtcg tcggcgcact
     4981 gcacgcacgc agcggggcgc accgggtcac gacccgggaa ggcgggtggc tccgaccgcg
     5041 acgacgcgcc gaggatccag gccggtgggc gacccgccgc ggcggcagcc ttgccgccgt
     5101 cctcgtcggt gagcaccacg gcgcaggcac cgtcggacga cggacacgac tcgaggaacc
     5161 ggatcgggtc ccacatcatc ggcgacgcct tcaccttctc gatcgagatg tcctcgatct
     5221 tgaggtgggc gtaggggttc ttgagggcgt tgagccggtc cttgaccgcg accttccaac
     5281 cgatgtgctc cggcgctccg ctgcggtgga tgtacgagcg gatgaacggg gcgaacgcgc
     5341 cgccggcacc gagggacgca cccttgccgc tgccgagcgc gaactgggcg ttgccctcgg
     5401 actgcttctc gaacgcgacg gcgagcacgg tgcggtgctt gccggtctgc acgtgcgacg
     5461 cggcgacgat tgccgtcgtg cccccgaccg aaccggccgt gtgcacccgg aacatcggct
     5521 tgcccacggc cccgagggcg tcggacaggt acagctcggg cttcatcacc ccttcgaaca
     5581 ggtcgggcgc cttgccgagg accacggcgt cgatgtcgga catcgtcatc tccgcgtcgt
     5641 cgagggcacg gaacaccgcc tctcggacca gaccgccgaa ggacacgtcg cggcggcggg
     5701 acttgtggtg cgtctggccg acgccgacaa ccgcggtggg gatcatgagt ggccgccctc
     5761 gttcgagtcc tcgagtatgc agatcaggtt ctgttgtagg cacggacccg acgtcgagtg
     5821 ggcgagggcc cggtgaccac cgccgaagat gtggtcggcg gcgtggccga tgcggcagag
     5881 gcccgtggcc atgatggggt tggccttgag tgcgccgccc gatgcgttga cggtcacctc
     5941 gtcgcccagg ccgagcaccg tgcgcagcag gagctcctcg tgggtgaacg ccgcctggag
     6001 ttccgcgagc tcgacggggc cggacccgag gccggccgcg tcggcggcga tgcggaccga
     6061 cggcgcgtcg tcgagcgctc ggaatgacgg gttgtggcac tcggtccggt ggtcgaatcc
     6121 ggtgatgtag gcgggccgtt cgacgagttc ccgggcccgg tcgccccggg ccagcaccat
     6181 ggcggcggcg ccgtcggtga tcggcggcac gtcgtggcgg cggagcggct cgcgcacgta
     6241 ctcctcgccg aggagggtgt cgatcgtctt cccgccgttg ccttcggggc cccgggaccg
     6301 catggcgacc tcggccatcg aacgttcgtc ggcgaggccg gcggcgatga cggcgcgggc
     6361 ctgcagcgcg gcgagcgtca acgggtcggc accgaggggt gcgaggaagt acgggtccat
     6421 ctccatcggg tagatcagcg acgggtcgcc ggtggacgag cgccccgagc cggtgacgac
     6481 ggcgatgtcg atgtcgccct cctggagccg gagccacgcc tcgtagagcg cccacgcgcc
     6541 gtccatctcg acgtggctgt cgcgtttggg cggccacgca ccgatggcgt cgaggttcga
     6601 cacgaacgag aacgcctgcc cggtgatgta gtcgcagctg ccgaggcacg tgaagtcgac
     6661 gtccgcccgc gtcaggccca acgggtcgag cgcggcggtg atgacgtcca tcagcatctg
     6721 tgtctcggac tgcttggtgt gtcgcagcat gggtgactgc gcccacccga cgacagcaat
     6781 gtcggtggag gtggccgaga ccatcaaaag atccggttca cgaggtcggg gtcgtcgatc
     6841 gagggttcgc cgctcgggat ccagccgagg aggctgccgt aggcgccgcc catcgcgccg
     6901 ccgtcctcgg tctcctcggc gggcgacgcc cacaccgccg tcacgcgttg gccgacgtgc
     6961 acttcgtcgt agggaagctc gatgaccggt tggtacgaca agatgacgtc ggtgtcgtcg
     7021 aggagcacga acgctcggac gaacggctcc gtttcggtct gtccggggta ctggaccggt
     7081 gtgacgatgg cgaagttggt gatggtgccc gactgcggga ggtcgacctc gtcggcgggg
     7141 ccgatctcga cggcgtcgat cgggcagtag ccgcggcccc ccgagtagat ccgcccgcac
     7201 acggggcact tcgaccccaa caaccgcgct tcacgcgatg ccgcaacggc tcgaaccgtc
     7261 gcagccggga cgggattgcg gtacgtgatc gaggcgacgt agtccatcat ctcgacgggt
     7321 tcgccggcgg tctcggtcag gtcggtgccg ccctcgatct cgggggtctc accggggacg
     7381 aagcacgcga tgtcgtcgag gcgaccgacc cgcgtggcct tccagcgggg agcgacgcgc
     7441 atcccggccg acatggcctc gggcgaaccg gcgtcgacgg cgtggagcag gggagtcgtc
     7501 gccccgtcga ggcggacgaa ggcgaacgag aaggcgtggc cgagcgggtg ctgctcggag
     7561 ggatgcggca cccacgtcca cgattcgacc gtgcccgccg gaccgacctc gatgaagtcg
     7621 tgggcgagct cggcgccggt cgccgggtcc cactccatcg gcggtacgag cacggcgtcg
     7681 ccgtttcgga tgccgaggat tcggcgctcg gtcagcgccg acatgaacgt gccgatcacc
     7741 ggaccgagcg agcggcggta cgggaacgtg atcgtgctct cgacgaagcg agtcacgaca
     7801 ggtctgctct cacgaggtac ccctgttctg cccgcccccc ggcggnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnncaccc gtgcgtgcga ggcggcacga gagatcgtcg
     7981 cgtccctcgg cctgcgtgtc gacgaggtga tcgtcctcca gaactcgaac aagctcacgc
     8041 tgcgcttgct gccgtgcgat gtggtcgccc gcgtgtccga cgtcgggcac gagcatgcgg
     8101 actccgagct cgagctcgcc agccggctgc tcgactccgg ttgccccgtg gccgcaccgg
     8161 agccgagagg gaggcggcgc ggccaccgca tcgacgggtt cgagatctcg ctgtggacgt
     8221 accacgagac ggcgacggca ccgatcacgc cggtcgccta cgcggccgcg ctcgaacgct
     8281 tgcattccgg catggcgtcc gtcgacgcca gggttcccca tgtcaccgac cggatcgcgg
     8341 gcgccgagcg gctcgtgacg aaccccatcc tcaccccgtc gctcaccccc gacgaccggc
     8401 tgctgctggt caccacgctg gagcagtcgc ggcgggcact ccgagatttc ggagcgccgg
     8461 agcagctgct gcacggcgag ccccaccccg gcaacctcat cggtactgcc gagggtccgg
     8521 tgttcatcga cctcgagacc tgttgtccgg gtcgacgagg tactccgtcg ccgcgacgca
     8581 ccccaagcgg gttcgatcta ggtgcggcga tgcccggccg tggaggtcag ctcgaaccac
     8641 accgtcttgc caccgacccg ttgggcgacg ccccacctcg acgagatctc gtcgagcaac
     8701 acgacgccgt acccgccggg tgtcatcggg tcgtccttga aggttcgtgc cggacgatcg
     8761 cgggtgcggt cgcggacgct cacgcggaca agttcggcgg aatgggtgac gtccacgacc
     8821 ggtcggtcgg taccaccgtg caacagtgcg ttcgttgcca cctcggacac gagcaactcg
     8881 gcgtgatcga cggcggggtc gccgtcccaa tcctcgagcg actcccggac ccacgcccga
     8941 gccatcgccg gtgacgaggc gacacatcgt gtgggcttgg tccgaacgta catgtcgtgc
     9001 tcctacccga tgctctgctg atcagtccgc agtgtgagtg gtgccccgtc cgggggaccc
     9061 gcgcttcgga accgctccgg tggggaagca ctaccccatg atcaaaaagc ttcctggact
     9121 cgtttggatc ctgttcctcg tcgcgatggc ggagcgcaag ctcgcgcccg ggacgttccg
     9181 ccgtctcctc cagaccctgc catgagctcg atcgggaggc ggggcagcga cccgccccgg
     9241 aggaagtcgg ccgacccctc ggtaccctga tcgagacgtc tgacggggaa agaagagggt
     9301 cgacgaacat gccggtgatc actgcaacta ccgctccgaa ctggtgccga ggcgacatcg
     9361 aggaacgcgc ctggatcgtg ggcgccgagg tcgccgctcg gttcgccgac gaggtcgacc
     9421 gcgacgcgag gttccccgtc gaggccgtcg aggcgatgcg agatgccggc ctgctgtcgg
     9481 cgatggtgcc gatcgagctc ggcggtgccg gtgcgtcgct cggcacgatc gccacggcga
     9541 cccgtgccct cgcgtaccac tgctctgcgt cggccctcgt catggccatg caccagatcg
     9601 agatctggta cctcaaccag ttcgggaaca ccgacggtct ccgtggcctg ttgcgctcgg
     9661 tcgcgaccga cggatggctc atcgcgaacg ccaacagcga ggtcggtctg ggtggcgagg
     9721 tgggccggag cttctgcgcc gtggagcacg gcgagggcac gttccacttg gagaaggagg
     9781 cgctcgcgat ctcctacggc gcgcacgccg atgcgctgct gctcatcgcc cggcgcagcc
     9841 ccgaagctcc cgagaacgag caggtgatgg ttgccgcccg cccgggccgg ttctccctcg
     9901 aacagacctc gagctgggac acgatggggc tccgcggcac gtgttcgtcg agcttcaaga
     9961 tcgtggtgga cgaggccgcg gacatggtct tccccacgcc gtggctgacc atcggctccc
    10021 gctgtgtcgg ggccacgacc atcctgctca actccacgtg gctcggcatc gccgaggcgg
    10081 ccgcagcgcg ggcgcacgcg ttcgtgcggg ccgacgcccg ccgcaagatc ggcacgctgc
    10141 cggcggctgc accgctcctc ggcgaggtgt cggtcaagct cggcgaggcc cgcgccgtca
    10201 tggactcgtc ggtgcgtgcc tacgaggctg ccgtggggac cgaggacatc gacgccacgt
    10261 cgctgctcct caccgttcgc aatctgaagc tgtcggccgc cgacctcgcc atcgagatcg
    10321 tcctcaaagc gtctcgcatc tgtggcctcg ccggctacaa gcgcgacacc ccctacgcca
    10381 tggaccgcaa cctgcgcgac gtcatcggtg gaccgttgat ggccaacaac ctgcgcgccg
    10441 ccggcgacaa cgcccagctg ctcctgggaa tgaagcagtt ctgaggctct gcccggtcag
    10501 tcctcgtcgt actcgatgag cgcggcggtg agcacggccg tgggcaccga cccgaggacc
    10561 gcgaggtcgt cggcggtgcc gctgacgcgc agcacgtaac cctggctgcc gttgcgccag
    10621 tcgagcgcgt cgcccgtcga acggttgggt gacaccgact cggcaccggc gacgccaacg
    10681 atcgcgtcca gcccgccgat ggcgcggagc gccgtggccg tcgtcggggg ttgggcgaag
    10741 aactcgtaca ggaacgggcc gtcggccgac gccgggagtt cggcgatcgc gtcgggggcg
    10801 atgccgagcg cgattgccgc ggcgagttcg gtgagcgagg tgccgaaccg cctggcgtac
    10861 agcgcatcga tggcgccccc gccgatccgg ccgttgacct cgatgatgcg gggcccgtcg
    10921 ggggtgagct tgatctcggt gtgcagcgcg cccgagcgga ccccgagcga ccgcgccgcg
    10981 gcgccggaca gatcgatgac ctccgaagcg ccggcggcgt cgagtgggtg cggcatgaag
    11041 ttgcccgttt cgcggaacgg gtgggcgagc gggaacttgc ccgtgatcgc caccgggatg
    11101 acctcgccgt cgacgaccag cgactcgacc gacacgtagc cgccgatgcg gccgttcgct
    11161 gcgggttcgc ggtcggcgag gtactcctcg acgatgaggt cgcccgtgac gtcgcgcgtc
    11221 gcgacgaccg tgagcgccgc ctgcagctcc gctcggtcgg cgacggcgac gacgtcgcga
    11281 ctgctgtcgc cacgcagtgg cttgaggacg agcggagtgc ggaggcccga tgcgagcgcg
    11341 gcgccggcgg ccgggtcgga gccggtgggg atccggacga aggcgggcac ggcgaggccg
    11401 gcgtcgagca tcgcctggcg ctggtggtac ttgtcgttga gtcgcgtcac cgccgacatg
    11461 gggttgccgg gcaggccgag cacgtcggcg atcgtggcgg ccagcaccag ctgggagtcg
    11521 gtgaaggcga tgaccccagc gagggggtga ccttcgaacc gggcatcgac gagcgctgca
    11581 gcgacctcgg cgggaggccg gccctcggtg tcgaacacgg tgccggcgcg ggtgaggagg
    11641 cgtcgcatgg gtccgagggc ctcgtcgccg gcgtcgacga tccacacgat gcggcacagg
    11701 tcgccgaggc gcgtcagcag ttccatcggc gacgacgacg tgaggtcgta ggtgaccccc
    11761 aggtacggga ggtcgacggg ccggtcggca gcgtcggtgg gcttgctctc ggtgggctcg
    11821 ctctcggcgg ggacggtcac cacggccacg gtagagggga gacggcgatc tccggcggaa
    11881 ccgctccgac gctgccgaat cgtgcttccc tttcgatgca tctcgtggtg cgatgcgtga
    11941 catcagccag ggcgaaccgg attattgctc aggcgggcac cggggatcgg tcgatggttg
    12001 gggttcgacg agagaggttc gggggtccgc gtgccggatc gacagcagag cagaggcggc
    12061 gtgcgtatgg cgtgctcggt cgacaacttc tggttgccga ccggcgacgg tgccacgggc
    12121 gcgtgctggt tctggccgga gggcgccgtc gcgtccaccg gtgtcctcgt tgtcccgggc
    12181 atcatccacg aacagcagac gatggccgtc gggctcgtgg cgctcgcgaa ggagttggcg
    12241 tcgaacggtg tcccgaccct gatgatcgac ctcgccggga cggcgcaggg taccggccgc
    12301 ctcgacagcc cccggaccgg ccagcggtgg gacgacgagg tccgctccgc cgtccaccac
    12361 atgcgtgacg ccgggctgca gcacatcgtg gtgctcggag ttcggttggg tgcgctcatc
    12421 gccgcccacg cgaccgtcga cgaaccggtc gacctcatgg tcatgtgggc gccggtgctc
    12481 tcggggcggc gatacgttcg cgagctccag ctcatgcagg cgtcgg
//
LOCUS       DAOSOA010000060        12516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5031_length_12516_cov_5.923682, whole genome shotgun sequence.
ACCESSION   DAOSOA010000060 DAOSOA010000000
VERSION     DAOSOA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12516
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5031_length_12516_cov_5.923682"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PKY13_05765"
     CDS             complement(<1..1139)
                     /locus_tag="PKY13_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT79472.1"
                     /translation="MMDFPLTLTHLFDRAERLFPRKEIVTASPTGRQRISYGEWAERT
                     RRLGTVLDDLGISADGRVATFAWNTSRHLELYFAAPCSGRVCHTLNLRLFPEQLVYIV
                     NHAEDEVIFVDNSLAKLLWPALETFTTVRHVVVMNDTGGDLPTDAPNGIEVHDYETLL
                     AAAAPREWDIPDENLAAAMMYTSGTTGNPKGVVYTHRSITLHTFGAMMTDSLGAHEKD
                     IIMPVVPMFHANAWGLAHAGVATGATLVMPGPDLSPGALITLIEEERVTVAAGVPTIW
                     MGVLGEIDGRDMSSLRAIPCGGSAVPPVLSEGFRAKIGLPVFQAWGMTETSPIAAACH
                     IKSYLEDTADRRATVGLVVPGVEFRTVVPNTTEIVPWDGETTGEL"
     gene            1244..1924
                     /locus_tag="PKY13_05770"
     CDS             1244..1924
                     /locus_tag="PKY13_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT79473.1"
                     /translation="MSYPAEPASPSFTPTDHTRVRYSKRASYDRSEAYRILDDGFVAH
                     VGFVFDGRPFVIPMVYGRDDDQLLLHGSVATRLMRALDHGAEACITVTHIDGLVLARS
                     HFHHSINYRSVVVLGTATRLRDADAEAAFEVIVDHVAPGRAAETRPTNDVERRQTMVL
                     RVPIDEASVKVRTGPPGDDPADLGEGSAEVWAGVLPLALVPGTPVPDEFTGADVVAPG
                     SVAPWRRP"
     gene            complement(1943..2371)
                     /locus_tag="PKY13_05775"
     CDS             complement(1943..2371)
                     /locus_tag="PKY13_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOT79474.1"
                     /translation="MKVVVIYESRTGNTERAAQLIAARFAATGHDVKLYPTKGVDLHE
                     LSEADLAVVGTWTDGAVLFGQRPGGGGNIAKYLPTMWDKPTYAFVTYAIRPGGVLRGF
                     NKLLASKGAKVLGSLELHRKHLDTDAPDFVDGVLAAFTNA"
     gene            2480..3394
                     /locus_tag="PKY13_05780"
     CDS             2480..3394
                     /locus_tag="PKY13_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOT79475.1"
                     /translation="MGHELVGGQALVEGVMIRQGPRWAAAARRPDGSIATTTRHAAPA
                     FAATRGIPVVRGIGALVDSVRIGLAAMRWSREETIDPTSADAAPVGGARERVVVLGVV
                     AGVLAAFLLVPLAIAALARPVVGTGWGAAVVEGLVRLALFVAYLAMLSTLPGVRRTLE
                     YHGAEHMVIAAHEHGEDRTIDSAWDYSVRHPRCGTDFFLLIFVISIVAFALVGHLPAA
                     ALVLSRILLAPVVVGVAYEILRLGGTSEHDGLAAALSAPGMFLQRFTTRQPDDAQIAV
                     ALAALDELLPPSASIGTTSRSNRTTSPL"
     gene            3444..4799
                     /locus_tag="PKY13_05785"
     CDS             3444..4799
                     /locus_tag="PKY13_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT79476.1"
                     /translation="MTTIDRSRLASLRQREERRFVDTHPRSAEASTTTGHLLGGVPMA
                     WMTRWPGAFPIVVDEASGARFRDIDGHEYIDFCLGDTGAMAGHALPAVADALGAQARR
                     GITTMLPSADAAWVADELASRFGLPTWQMAMTATDANRFVLRFARHLTGRSKVLVFDW
                     CYHGTVDEAFATLDDDGAVVARPGTIGAPVDLDATTKVVQFNDLDALEAALAPRDVAC
                     VLAEPALTNIGIVLPDPGFHDALREICDRTGTLLVIDETHTLCAGPGGATREWGLRPD
                     FFVIGKAIGGGMPVAAYGMSAEVATRLAPAIGGAEVDTSGVGGTLTGNALALAAVRAT
                     LSTTLTADDFARMIPLAAAWTDGVQATIDATAVPWCVNQLGCRAEYWFCPPPRNGRDG
                     AAAVDADLDAYLHLFALNRGILMTPFHNMALMSPAHSMADVDRHSEVFAEAVTELVAP
                     G"
     gene            4855..5715
                     /locus_tag="PKY13_05790"
     CDS             4855..5715
                     /locus_tag="PKY13_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT79477.1"
                     /translation="MALPDPMTAPTTSPNIDPTRPVVRDLDGQVAVVTGAGSPSGIGF
                     GIATHLAERGAVVVIGSTTERIHERVGELVEAGHRALGFIGDLVDATVCDRLVAMVAE
                     RIGRIDILVNNAGMTTVGDVADEAGATPAEGRIDDVAWRRAIDRNLTTAFNMSRAVAP
                     VMSAAGYGRIVNMASVSGTSMAYAGDAGYHAAKAGVAGLTRAMALELAPAGVCVNAVA
                     PGWIATGSSTAPELAAGAATPVGRPGTPSEVAAVVGLLASPAASYLCGQVIVVDGGNG
                     LEENRALRAS"
     gene            5771..6232
                     /locus_tag="PKY13_05795"
     CDS             5771..6232
                     /locus_tag="PKY13_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT79478.1"
                     /translation="MSGLPIELDDVDKRIVTELQRDGRISYADLGPIVGLSPAAVRQR
                     VQRILDAGVVQVVGVTDPSAVGYPVMAMLGVQVDGDVTQIADEIAAVDGVIYIVMSAG
                     SFDLMVEVICEDTMALLDLINRKVKTVEGVRQVESFVYFGIHTHRFGWGVR"
     gene            6270..6740
                     /locus_tag="PKY13_05800"
     CDS             6270..6740
                     /locus_tag="PKY13_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HOT79479.1"
                     /translation="MSTRKPTPFTPEQEARGDRFIKLMSRFNTGLYRRTGGRVGAKFP
                     GGAPVCLLTTTGRKSGEPRTMPLLYLRDGDDIVIVASKGGFSSHPQWYLNLLADPEVT
                     IEIKRTKLQMTARVADADEKAALWPKLVGMYSSFDQYQERTERDIPVVVCSPRT"
     gene            6737..7822
                     /locus_tag="PKY13_05805"
     CDS             6737..7822
                     /locus_tag="PKY13_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT79480.1"
                     /translation="MTDLAPNNRPEPEGRVPAGLVVASEWAWRLLLVVVGIGAVVYAA
                     AALGFIVGPVVVALVISALLEPVRRILLRWGASTTVAWLVAFLFGVVVVSSIMVLAIS
                     EFSANFDELSEQAAGGVDEFTNWLEDGPLHMDAGGLNDAVDKAFASIKDDPTEAITGT
                     VSVLSTTSSLLAAGVLTLLTTLFFMKDRSLMWMWFTRLFPERSRQRVDSAGRAGWEVL
                     ISYTRVTLTSAVVDSTVIGIASAIAGLPVAFALAVVVFLFAFIPTVGAIFSGLVVVLV
                     ALVSQGPTTALVMAIIVLIVQQLDANVMYPILASRHLALHPLASLLLVAGGGVVAGLF
                     GAFIAVPLAAVLLAVGGDVRRSSILRA"
     gene            7874..8245
                     /locus_tag="PKY13_05810"
     CDS             7874..8245
                     /locus_tag="PKY13_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79481.1"
                     /translation="MDDRLIARLVGAGRVLFGIACLVAPRQILRQWGKDASPAVLWWI
                     RAFGIRDTVLGAGALVSLNSDQPDTTWVKAGAAADTADAVVVLLGRNELDPSFVIPTL
                     AIAGPAAALGWKASLGLSKRR"
     gene            8272..8856
                     /locus_tag="PKY13_05815"
     CDS             8272..8856
                     /locus_tag="PKY13_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT79482.1"
                     /translation="MKAFLGRLFLKLWRWTPVGTKPARGSVIVCAPHTSNLDYLLMIF
                     TAWTQNMNLSFLGKSQLFKPPLGWLMRATGGIPVDRNAANGLVGQMTEAFTATPDLML
                     AIPAEGTRSKAEYWKSGFYRIAQSAEVPICLAFIDKSKREVGFGLVFEPSGDIAADME
                     RVRVFYDGKIGLKHHNFGPVRLREETPDEGAAAG"
     gene            complement(8935..9555)
                     /locus_tag="PKY13_05820"
     CDS             complement(8935..9555)
                     /locus_tag="PKY13_05820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT79483.1"
                     /translation="MAANVNIRLATEADLPELSQVLGRAFADDPVMEFVISSRPVAPR
                     AAILLGTITSLHISDRSVFVATDPASGAILGASVWGLPGHWRVPTTAYVRHARKLLKA
                     VGLRGLTKISVLSAIERQHPTEPHHYLAIIGTDPEHQGKGIGAALLAPVLNLCDEEGL
                     PAYLESSKDSNVPYYRRFAFEVTTPFTLKGGPTLHFMWRDPEVPTS"
     gene            complement(9567..10559)
                     /locus_tag="PKY13_05825"
     CDS             complement(9567..10559)
                     /locus_tag="PKY13_05825"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT79484.1"
                     /translation="MTGRPALPAATAPGRRLAAALLLGIGVLFALPALAGATGLTERA
                     ATARVAADSGDSLSTGDAIILGAVEGITEYLPVSSTGHLLITERLLDIGTGADKDATD
                     TYTVVIQVGAIIAVLGIFWKRFLSMASGVVGRDEDGRRTLISLLVAFVPAGVIGKVFG
                     DAVKEHLLSPTPIAITWIVGGIVILVFVSQQSRLVARIDSVADIGARNAAIIGVAQTL
                     ALIPGTSRSFVTILAAMLLGCSLATAVEFSFLLGFVTLSAATVFELLQNGDTLVDTFG
                     IASPLIGIVVAGITAFLSVKFMIDWLNKRGLAVFGWYRIAVGVAAIALIAGNRI"
     gene            complement(10556..11326)
                     /locus_tag="PKY13_05830"
     CDS             complement(10556..11326)
                     /locus_tag="PKY13_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79485.1"
                     /translation="MTQPESANPRIAWAIHIFTAVGIIAGFLALTSALDGSPRMSLTW
                     MTIAMVIDGLDGPFARKYEISAVLPHIDGHIGDLIIDYVTTVIAPAFFIHEFDLLPKQ
                     YSLLGISLMLLTSLYLFSNTHIQSNDNYFNGFPAMWNLVINVLFVLQSPPTTNLIVVL
                     VMSVFTIVPVKFIHPIRVRDFRKITIPVLVIWLAAMLYLTWIIDQRTRGCDSACLSSG
                     ARFAQALVYLGALWIVGVGIWRTFRGDPHDSPDVAAPA"
     gene            11374..12516
                     /locus_tag="PKY13_05835"
     CDS             11374..12516
                     /locus_tag="PKY13_05835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HOT79486.1"
                     /translation="MMSPTVLPLADEPIRHLYVHVPFCPTICPFCSFHVLERRSAAVD
                     AYLERLDAELAELADARRLELDTVYLGGGTPSYLRPSEMERLRRSIGHRVGWAGVEAT
                     LEVHPSTASPERAAQWAELGFNRLSVGAQSFDDGVLRTLGRPHDADAGRRAVEWCLAT
                     GATTSVDIMTAIDGQDLASDLLAAVDLGVDHVSAYTLTIEPGTPFERLGVQVPDERAA
                     DALVLTASVLAAAGFERYEVSNHAREGARSVHNRAYWTSAWFAGVGPSASSHLPMRDP
                     RDSSTIAVRSRNADTPAWLEGAAPEWEAVTGRDRLGDAMLCGLRLVDGIDLDELTVMT
                     GLDPRAELAGELAELVGDGLVEIDGAALRPTSAGLLVLDRVAAALL"
BASE COUNT         1819 a         4224 c         4479 g         1994 t
ORIGIN      
        1 tggagctcgc cggtggtctc gccgtcccac ggcacgatct cggtggtgtt cggcaccacg
       61 gtgcggaact cgacaccggg aacaacgagg ccgacggtgg cgcgccggtc ggcggtgtcc
      121 tcgaggtagc tcttgatgtg gcacgccgca gcgatggggg aggtctcggt catgccccag
      181 gcctggaaca ccggcaggcc gatcttggcg cggaagcctt cgctcagcac cggaggaacg
      241 gccgagccgc cgcaggggat cgcccgcagc gacgacatgt cccgtccgtc gatctcgccc
      301 aacacgccca tccagatggt gggtacgccg gcggcaaccg tgacccgctc ctcctcgatc
      361 aaggtgatga gggcgcccgg cgacaggtcg ggcccgggca tgacgagcgt ggcgccggtc
      421 gcgacgccgg cgtgggccag gccccacgcg ttggcgtgga acatcggcac caccggcatg
      481 atgatgtcct tctcgtgggc accgagactg tcggtcatca tggcgccgaa cgtgtgcagc
      541 gtgatcgagc ggtgggtgta caccacaccc ttggggttgc cggtcgtgcc cgacgtgtac
      601 atcatcgccg cagccaggtt ctcgtcgggg atgtcccact cgcggggagc ggccgcggcc
      661 aacagcgtct cgtaatcgtg gacctcgatg ccgttgggcg cgtcggtggg aaggtcacca
      721 ccggtgtcgt tcatgacgac gacgtgtcgc accgtcgtga acgtctcgag cgccggccac
      781 agcaacttgg cgagcgagtt gtcgacgaag atcacttcgt cctcggcatg gttgacgatg
      841 tacacgagct gttcggggaa cagccggagg ttgagggtgt ggcagacccg tccgctgcac
      901 ggtgcggcga agtagagctc gaggtgacgc gacgtgttcc aggcgaaggt cgcgacccgg
      961 ccgtcggcgg agatgccgag gtcgtcgagc acggtgccga gccggcgggt gcgctcggcc
     1021 cactcgccgt agctgatgcg ctgacgaccg gtgggcgagg cggtgacgat ctccttgcgc
     1081 gggaacagcc gctcggcccg atcgaagaga tgggtcaggg tgagcggaaa gtccatcatc
     1141 gtgccgcgca ttgcgtacct ccaggtgatt gccgggcccc cgtggccgac gtgggtccga
     1201 aactacgcga catggatcgc ggcgtgcggg cgatcatggc gcgatgtcat accctgccga
     1261 acccgcgtcg ccctcgttca ccccgaccga tcacacgagg gtccggtact cgaagcgggc
     1321 gagctacgac cggtccgagg cgtaccgcat cctcgacgac gggttcgtcg cccacgtggg
     1381 cttcgtcttc gacgggcgac cgttcgtcat ccccatggtg tacgggcgcg acgacgacca
     1441 gctcctcctg cacgggtcgg tggccacgcg cctgatgcgg gccctcgacc acggtgccga
     1501 ggcctgcatc accgtgaccc acatcgacgg gctggtgctc gcccggtcgc acttccacca
     1561 ctcgatcaac tatcggtcgg tcgtggtgct cggaacggcg acgcgactgc gcgatgcgga
     1621 cgccgaggcc gcgttcgagg tgatcgtcga ccacgtcgct cccggtcggg cggccgagac
     1681 ccgtccgacc aacgacgtcg agcgccgtca gacgatggtg ctgcgggtgc cgatcgacga
     1741 ggcgtcggtg aaggtgcgca cggggcctcc cggcgacgat ccggccgatc tcggcgaggg
     1801 ctcggccgag gtgtgggccg gggtgcttcc gctcgcgctc gtgccgggga caccggtacc
     1861 cgacgagttc acgggagccg acgtcgtcgc ccccggttcg gtcgcgccgt ggcggcgacc
     1921 ctgacgccga ccgtgtcggc gatcaggcgt tggtgaacgc ggccaacacc ccgtcgacga
     1981 agtcgggggc gtcggtgtcg aggtgcttgc ggtgcagctc gaggctgccg agcaccttgg
     2041 cgcccttcga tgcgagcagc ttgttgaagc cgcgcagcac accaccggga cggatcgcgt
     2101 aggtgacgaa ggcgtaggtg ggcttgtccc acatggtcgg caggtacttg gcgatgttgc
     2161 cgccgccgcc gggtcgttgc ccgaagagca cggcaccgtc ggtccacgtg cccaccacgg
     2221 cgaggtcggc ctcggagagt tcgtggaggt caacgccctt ggtggggtac agcttgacgt
     2281 cgtggcccgt cgccgcgaac cgcgccgcga tcagctgggc ggctcgttcg gtgttgccgg
     2341 tacggctctc gtagatcact actaccttca tcgcccctga tcctggccga aaagaggggt
     2401 ccggcgccag catcacgggg ggaggaatcg tccggatggc ccgtttcggt tcgggaacgt
     2461 gctcggtacc ctccgcaggg tgggacatga actcgtcggt ggtcaggcct tggtcgaggg
     2521 cgtcatgatc cgccagggcc ctcgatgggc tgcggcggcg cgacgccccg atgggtcgat
     2581 cgcgaccacg actcgccatg ccgccccggc gttcgccgcc acacggggca tccccgtcgt
     2641 gcgcgggatc ggtgctctgg tcgactccgt gcggatcggg ctggctgcga tgcgttggtc
     2701 gcgggaggag acgatcgacc ccacctcggc cgacgctgcg ccggtcggag gggcccgcga
     2761 acgggtggtc gtcctgggcg tggttgccgg ggtgctcgcc gcgttcctgc tggtaccgct
     2821 cgccatcgcc gcgttggcgc ggcccgtcgt cgggacgggc tggggtgccg ccgtggtgga
     2881 aggtctggtg cgactcgcgc tgttcgtcgc ctacctggcg atgctgtcga cgctgcccgg
     2941 tgtgcggcgc acgttggagt accacggggc cgagcacatg gtgatcgctg cgcacgagca
     3001 cggtgaggac cgcacgatcg acagcgcctg ggactacagc gttcgtcacc cccggtgcgg
     3061 caccgacttc ttcctcctga tcttcgtgat ctcgatcgtc gccttcgcgc tcgtcggcca
     3121 tcttccggca gcggcgctgg tgttgagccg aatcctgctc gcccccgtgg tggtgggcgt
     3181 cgcgtacgag atccttcgcc tcggcggcac ctccgagcac gacgggttgg cagcggccct
     3241 gtccgcaccc gggatgttcc tccagcggtt cacgacccgc cagcccgacg acgcccagat
     3301 cgccgtggcg ctcgccgcgc tcgacgaact gctcccaccg tcggcgtcga tcgggacgac
     3361 ctcacgatcg aatcgaacga catcgccgct ctagacacag gaatcgcttg acgaagtcgg
     3421 ttgagggggc agattcttgt gtgatgacga ccatcgaccg ttcccgtctc gccagccttc
     3481 ggcaacggga ggagcgacga ttcgtcgaca cccacccgcg atccgccgag gcctcgacga
     3541 cgaccggtca cctgctgggc ggcgtgccga tggcctggat gacgcgttgg cccggagcgt
     3601 tcccgatcgt ggtcgacgag gcgtcgggcg cacggttccg tgacatcgac ggccacgagt
     3661 acatcgactt ctgcctgggt gacacggggg cgatggccgg ccacgcgctc ccggcggtcg
     3721 ccgatgcgtt gggggcccag gcccgacggg gcatcacgac gatgctgccg agcgccgacg
     3781 cggcgtgggt cgccgacgaa ctggcgagcc ggttcggcct ccccacctgg cagatggcca
     3841 tgaccgccac cgatgccaac cggttcgtgt tgcgctttgc ccgacacctc accggccgtt
     3901 cgaaggtcct ggtcttcgac tggtgttacc acgggacggt cgacgaggcc ttcgccaccc
     3961 tcgacgacga cggcgccgtg gtcgcccgac ccggcaccat cggggctccc gtcgacctgg
     4021 acgcgacgac gaaggtcgtg cagttcaacg acctcgatgc gctcgaggcg gcgctcgcgc
     4081 cccgggacgt ggcctgtgtg ctggcggagc cggcgctcac caacatcggc atcgtgctgc
     4141 ccgacccggg gttccacgac gcactccggg agatctgcga tcgcaccggc accctcctgg
     4201 tgatcgacga gacccacacg ttgtgcgccg ggccgggcgg ggcgaccagg gagtgggggc
     4261 tgcgtcccga cttcttcgtg atcggcaagg cgatcggcgg tgggatgccc gtcgccgcct
     4321 acggcatgtc tgccgaggtc gccacgcgtc ttgccccggc gatcggcggc gccgaggtcg
     4381 acacgtcggg tgtcggaggc acgctcaccg gtaacgccct ggccctggcg gccgtgcggg
     4441 cgacgctgtc gaccacgctc actgccgacg acttcgcccg catgatcccg ctcgcggcag
     4501 cctggaccga cggcgtgcag gccacgatcg acgcgacggc cgtcccgtgg tgtgtcaacc
     4561 agctggggtg tcgggcggag tactggttct gcccgccgcc acgcaacggc cgcgacggtg
     4621 ccgcggcggt cgacgccgat ctcgacgcct acctccacct cttcgcgctc aaccgcggca
     4681 tcctcatgac gccgttccac aacatggcgc tgatgtcgcc ggcgcactcg atggccgacg
     4741 tcgaccgtca cagcgaggtg ttcgccgagg ccgtcaccga gctcgtcgcg ccgggatgag
     4801 cgaccgcggg gtcacgcgtc cggaggtgtg gcgggatcgt ggtacaaccg ctgcatggcc
     4861 cttcccgacc cgatgaccgc cccgacgacg tccccgaaca tcgacccgac ccgccccgtc
     4921 gtgcgtgacc tcgacggtca ggtcgccgtg gtcaccggtg cgggcagccc gtcgggaatc
     4981 ggcttcggaa tcgcgaccca tctcgccgag cgtggggcgg tggtcgtcat cgggtcgacc
     5041 acggagcgca tccacgagcg cgtcggcgag ctggtcgagg ccgggcaccg ggccctcggc
     5101 ttcatcggcg acctcgtcga cgcgaccgtg tgcgaccgcc tcgtcgccat ggtggccgag
     5161 cgcatcggcc gcatcgacat cctcgtgaac aacgcgggga tgaccaccgt cggcgacgtc
     5221 gccgacgagg ccggggccac ccccgccgag ggtcgcatcg acgacgtggc ctggcgccgg
     5281 gcgatcgacc gcaacctcac gaccgcgttc aacatgagcc gggccgtcgc cccggtcatg
     5341 agcgccgcgg gctacggccg catcgtcaac atggcgtccg tgagcggcac ctccatggcg
     5401 tacgccggtg acgccggcta ccacgcagcc aaggcgggcg ttgccgggct cacccgggcc
     5461 atggcgctcg agctggcgcc cgcgggggtg tgcgtcaacg cggtggcgcc gggctggatc
     5521 gcgaccgggt cgtccaccgc tcccgagctc gcggcgggtg cagccacgcc ggtgggccgc
     5581 cccggcacgc cgtcggaggt cgcggccgtc gtcgggctcc tcgcctcccc ggcagcgtca
     5641 tacctctgcg gtcaggtgat cgtcgtcgac ggcggcaacg ggctcgagga gaaccgggcg
     5701 ctgcgagcgt cctgaccgga ccggtgacag aaccgctggc ggcccgccga tctgtgccag
     5761 gctcgacgag gtgagcggcc tgcccatcga gctcgacgac gtcgacaagc gcatcgtcac
     5821 cgagctgcag cgcgacggcc ggatctccta cgccgacctg ggcccgatcg tcggcctgtc
     5881 gcccgcggcc gtccgccaac gggtgcaacg catcctcgac gccggtgtcg tgcaggtcgt
     5941 gggggtcacc gacccgtcgg ccgtcggata cccggtgatg gcgatgctcg gcgtgcaggt
     6001 cgacggcgac gtgacccaga tcgccgacga gatcgccgcc gtcgacggcg tcatctacat
     6061 cgtcatgtcg gccggcagct tcgacctgat ggtcgaggtc atctgcgagg acacgatggc
     6121 gctgctcgac ctgatcaacc gcaaggtcaa gacggtcgag ggtgtccgcc aggtcgagag
     6181 cttcgtctac ttcggcatcc acacccaccg cttcgggtgg ggcgtccggt gacgcgtccg
     6241 gcgccgcgtc cgcttccgag gagcacccca tgagcacccg caaacccacg cccttcaccc
     6301 ccgagcagga agcgcgcggc gaccggttca tcaagctgat gtcccggttc aacaccgggc
     6361 tctaccgccg gaccggcggc cgcgtcggcg ccaagttccc cggtggcgca ccggtgtgcc
     6421 tgctcacgac gacgggacgc aagagcggcg agcccaggac catgccgctg ctctatctgc
     6481 gcgacggcga cgacatcgtg atcgtcgcgt ccaagggcgg attctcgagc cacccgcagt
     6541 ggtacctcaa cctcctcgcc gatcccgagg tcaccatcga gatcaaacgc acgaagctgc
     6601 aaatgacggc acgggtcgcc gacgccgacg agaaggcggc gctgtggccg aagctcgtgg
     6661 gcatgtactc gtcgttcgac cagtaccagg agcggaccga gcgcgacatc ccggtcgtgg
     6721 tctgctcgcc gaggacatga ccgatctggc gccgaacaac cggcccgagc cggagggtcg
     6781 cgtccccgcc ggcctcgtcg tggcctcgga gtgggcgtgg cggctgctgc tcgtcgtggt
     6841 gggcatcggg gccgtcgtct acgcggcggc tgccctcggg ttcatcgtcg gccccgtcgt
     6901 cgtcgcactg gtcatcagcg cgctcctcga gccggtgcgc cggatcctgt tgcgttgggg
     6961 agcgtcgacg acggtcgcgt ggttggtcgc gttcctgttc ggggtcgtcg tcgtctcgag
     7021 catcatggtc ctcgcgatca gcgagttctc cgcgaacttc gacgagttgt ccgagcaggc
     7081 ggccggtggt gtcgacgagt tcaccaactg gctcgaggac ggtccgctcc acatggacgc
     7141 cggtggcctc aacgacgccg tcgacaaggc cttcgcctcg atcaaggacg accccaccga
     7201 ggcgatcacc ggcacggtgt cggtgctgtc caccacgtcg agcctgctcg ctgccggggt
     7261 gctgaccctg ctcacgacgc tgttcttcat gaaggaccgg tcgttgatgt ggatgtggtt
     7321 cacgcgcttg ttccccgaac ggagccggca gcgcgtcgac tcggccggcc gagccggctg
     7381 ggaggtgctc atcagctaca cgcgggtgac cctcacgtcg gccgtcgtcg actcgacggt
     7441 catcggcatc gcctcggcga tcgccggcct gccggtggcg ttcgcgctgg cggtggtggt
     7501 gttcctcttc gcgttcatcc cgacagtcgg ggcgatcttc agcgggctcg tggtggtgct
     7561 cgtggccctc gtgtcgcaag gcccgaccac ggcgctggtg atggcgatca tcgtgctgat
     7621 tgtccagcag ctcgacgcca acgtgatgta cccgatcctc gcgagccgcc acttggccct
     7681 ccacccgttg gcgagcctgc tgctcgtggc cggtggtggt gtcgtcgccg ggctgttcgg
     7741 ggcgttcatc gcggtgccgc tcgctgcggt gctccttgcg gtcggtgggg acgtccggcg
     7801 ctcgtcgatc ctgcgggcct gacccccgca ccgtcggtcg caggggtcgc ggcggtgtgc
     7861 aacgatgcag gccatggatg accgtctcat cgcccgactc gtcggcgccg gccgtgtgct
     7921 gttcggcatc gcctgcctcg tcgccccccg tcagatcctg cgccagtggg gcaaggacgc
     7981 ctcgccggcg gtcctgtggt ggattcgtgc cttcggcatc cgtgacaccg tgctgggcgc
     8041 cggtgcgctc gtgtcgctca acagcgatca gcccgacacc acctgggtga aggcgggcgc
     8101 agcggccgac accgccgacg ccgtcgtcgt gctgctgggc cgcaacgagc tcgacccgtc
     8161 gttcgtgatc ccgacgctcg cgatcgccgg ccccgccgct gccctcgggt ggaaggcgtc
     8221 cctcggtctg tcgaagcgtc gctgacatcc cccgctgtta gggtcgcggg cgtgaaagcc
     8281 ttcctcggtc gtctgttcct caagctgtgg cgttggacgc cggtcggtac caagcccgcc
     8341 cgtgggtcgg tcatcgtctg cgcaccccac acgtcgaacc tcgactacct gttgatgatc
     8401 ttcacggcgt ggacgcagaa catgaacctg tcgttcctcg gcaagtcgca gctgttcaag
     8461 ccgccgctcg gctggctcat gcgcgccacc ggcggtatcc ccgtcgaccg caacgccgcc
     8521 aacgggctgg tgggccagat gaccgaggcg ttcacggcga cgccggatct gatgctggcg
     8581 atccccgccg aggggacccg aagcaaggcc gagtactgga agtcggggtt ctaccgaatc
     8641 gcccagtcgg ccgaggtgcc gatctgcctg gcgttcatcg acaagtcgaa gcgtgaggtc
     8701 gggttcgggc tggtgttcga gccgagcggc gacatcgccg ccgacatgga gcgggttcgg
     8761 gtgttctacg acggcaagat cgggctcaag caccacaact tcgggccggt gcgcctccgt
     8821 gaggagaccc ccgacgaggg tgcggccgcc ggctgactcc cgttccccgg tgttttgtag
     8881 tgcgaggagc gacaccagcg tcgtctgtcg cagcacaaaa cctcctgagg tgggtcagct
     8941 cgtcggtacc tccgggtcgc gccacatgaa gtgcagggtg ggcccaccct tgagcgtgaa
     9001 cggtgtcgtg acctcgaacg cgaaccgccg gtagtacggc acgttcgaat ccttcgacga
     9061 ttcgaggtac gcgggcaggc cttcctcgtc acagaggttg agtaccggcg ccagcagcgc
     9121 cgccccgatg cccttgccct ggtgctcggg gtccgtcccg atgatcgcca ggtagtggtg
     9181 cggttcggtc gggtgctgtc gctcgatcgc cgagagcacg ctgatcttgg tgaggccgcg
     9241 taggcccacc gccttgagca gcttgcgagc gtggcggacg taggcggtcg tggggacgcg
     9301 ccagtgcccg ggcaggcccc acaccgaggc gcccaggatc gcgcccgagg ccgggtcggt
     9361 cgcgacgaac accgagcgat ccgagatgtg gaggctggtg atcgtgccca gcaggatggc
     9421 ggcgcggggc gcgacgggac ggctgctgat gacgaactcc atcacggggt cgtcggcgaa
     9481 ggcccgtccc agcacctgcg acagttcggg gaggtcggct tcggtggcga gccggatgtt
     9541 cacgttcgca gccatgccgg tgaagctcag atccggttgc cggcaatgag ggcgatggcg
     9601 gcgacgccga ccgcgatccg gtaccagccg aacacggcga gcccgcgctt gttgagccag
     9661 tcgatcatga acttgaccga caggaaggcg gtgatgccgg ccaccacgat gccgatgagc
     9721 ggcgaggcga tgccgaaggt gtcgacgagg gtgtcgccgt tctgcagcag ttcgaacacc
     9781 gtggctgccg agagggtgac gaacccgagc aggaagctga actcgaccgc ggtggcgagg
     9841 ctgcagccga ggagcatcgc ggcgaggatc gtgacgaagc tgcggctcgt gcccgggatg
     9901 agcgcgaggg tctgggcgac gccgatgatc gcggcgttgc gcgccccgat gtcggcgacc
     9961 gagtcgatgc gggccaccaa ccgggactgc tgggagacga agaccaggat gacgatgcca
    10021 ccgacgatcc aggtgatcgc gatcggcgtc gggctgagca ggtgctcctt gacggcgtcg
    10081 ccgaacacct tgccgatgac gccggccggt acgaaggcga cgagcagcga gatgagcgtg
    10141 cgccggccgt cctcgtcgcg accgaccaca ccgctcgcca tcgacaggaa gcgcttccag
    10201 aagatgccga ggacggcgat gatggccccc acctggatga ccacggtgta ggtgtcggtg
    10261 gcgtccttgt cggccccggt gccgatgtcg aggaggcgct cggtgatgag caggtggccc
    10321 gtcgagctga ccggcaggta ctcggtgatg ccctcgacgg ccccgaggat gatcgcgtcg
    10381 ccggtggaca gcgaatcgcc ggagtcggcg gcgacacgcg ccgtcgcggc tcgttcggtc
    10441 agaccggtgg cgccggcgag cgccggaagg gcgaacagca cgccgatgcc caagagcagc
    10501 gccgcggcga ggcggcggcc cggcgccgtc gcagccggaa gagcgggtcg gccggtcatg
    10561 ccggggccgc cacatcgggc gagtcgtggg ggtcaccgcg gaacgtccgc cagataccca
    10621 cacccacgat ccacagggct ccgaggtaca cgagcgcctg ggcgaagcgg gccccggacg
    10681 agaggcacgc cgagtcgcag ccccgggtgc gctggtcgat gatccaggtg aggtagagca
    10741 tcgccgccag ccagatcacc aggacgggga tcgtgatctt gcggaagtcg cggacccgga
    10801 tcgggtggat gaacttgacc ggcacgatgg tgaacacgct catcacgagc accacgatga
    10861 ggttggtcgt gggtgggctc tggaggacga acagcacgtt gatcaccagg ttccacatgg
    10921 cggggaaccc gttgaagtag ttgtcgttgc tctggatgtg cgtgttggag aacaggtaga
    10981 gcgacgtcag cagcatcagg ctgatgccga gcaggctgta ctgcttgggg agcaggtcga
    11041 actcgtggat gaagaacgcc ggggcgatga ccgtcgtgac gtagtcgatg atgaggtcgc
    11101 cgatgtggcc gtcgatgtgg ggcaggacgg cggagatctc gtacttgcgg gcgaacggcc
    11161 cgtcgaggcc gtcgatcacc atcgcgatgg tcatccacgt caacgacatc cgcgggctgc
    11221 cgtcgagggc ggacgtgagc gccaggaacc cggcgatgat gccgacggcg gtgaagatgt
    11281 ggatggccca ggcgatgcga gggttggcgg actcgggctg ggtcatttgc ccgtagatta
    11341 gagcgccggc cgtcccggcg gcacgatcgc cccatgatgt ccccgaccgt cctgccgctc
    11401 gccgacgagc cgatccggca tctgtacgtg cacgtgccgt tctgcccgac gatctgtccg
    11461 ttctgcagct tccacgtgct cgagcgccgg tcggcggccg tcgatgccta cctggagcgg
    11521 ctcgacgccg agctggcgga gctggccgac gcccgccgtc tcgaactcga cacggtgtac
    11581 ctcggcgggg ggacaccgtc ctacctccga ccctcggaga tggagcgcct gcgccgcagc
    11641 atcggccatc gtgtgggctg ggccggcgtc gaagcgaccc tggaagtgca cccgtcgacg
    11701 gcctcacccg agcgggcggc gcagtgggcc gagctggggt tcaaccggct gagcgtgggc
    11761 gcgcagtcgt tcgacgacgg ggtgctgcgc acgctggggc gcccgcacga cgccgatgcg
    11821 gggcgccgcg ctgtcgagtg gtgcctggcg acgggcgcga cgacctcggt ggacatcatg
    11881 accgccatcg acggccagga cctcgcctca gacctcctcg ccgccgtcga tctcggcgtc
    11941 gatcacgtgt cggcgtacac cttgaccatc gagccgggaa caccgttcga gcgcctcggg
    12001 gtgcaggtcc ccgacgagcg tgcggccgac gcgctcgtgc tgacggcgtc ggttctcgcc
    12061 gccgccgggt tcgagcggta cgaagtgtcc aaccacgccc gggagggcgc ccgatcggtc
    12121 cacaaccggg cgtactggac gagcgcctgg ttcgccgggg tcgggccctc ggcatcgagc
    12181 catctcccga tgcgcgatcc ccgcgacagc tccacgatcg ccgtccgttc ccgcaacgcc
    12241 gataccccgg cgtggctcga gggagcggcg ccggagtggg aggcggtgac gggtcgcgac
    12301 cggctcggcg atgcgatgct gtgcggtctc cgtctggtcg acggcatcga cctcgacgag
    12361 ctgacggtca tgaccggcct cgacccgagg gccgagctgg ccggcgagct ggcggagctc
    12421 gtcggcgacg ggctcgtcga gatcgacggt gcggcgctcc gcccgacctc cgccggtctc
    12481 ctcgtgctcg accgggtcgc ggccgcactg ttgtag
//
LOCUS       DAOSOA010000061        12495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5043_length_12495_cov_5.835289, whole genome shotgun sequence.
ACCESSION   DAOSOA010000061 DAOSOA010000000
VERSION     DAOSOA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5043_length_12495_cov_5.835289"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PKY13_05840"
     CDS             <1..608
                     /locus_tag="PKY13_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT79487.1"
                     /translation="GNHVLGAAVAQIGAWNEEFGPGDFWVSCNVAPPQLDDSTFPDRV
                     MRILDGAPVAAHRLCVEVTEDSMMSTDRSKETLDRLRRSGVRIALDDFGTGYSSLVHL
                     IDVNFDILKLAGDLMPPHRGYGAATANGRQILGAVVNMAHSIGLDVVAEAVETEVQWD
                     ELASCGADLVQGWAFSKSRPPGDPFLDALVSGASARVGAVV"
     gene            complement(666..1958)
                     /locus_tag="PKY13_05845"
     CDS             complement(666..1958)
                     /locus_tag="PKY13_05845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT79488.1"
                     /translation="MSAARRHILHVDMDAFFVSVELLERPELRGRPVVVGGTGPRGVV
                     AAASYEARAFGVHSAMPSAVARRLCPHALFLDGRHERYREVSAVVMDIFGSITPLVEP
                     LSLDEAFLDVSGRVRALGPAPGIAATIRRRVLDEVGLTCSVGVAHNKFLAKLATETAK
                     PRATPTGPVFGSGVAVVDPDDVEAFLWPLAASDLWGVGPATLAKLERLGVATVGDIAR
                     LPLDTLCRAVGTASGTQLHRLSHGLDDRPVEPGQVAKSISHEETFPTDVTDRAHLERE
                     VVRLADAVADRLRRAGSYARTVQLKLRHGTFQTLTRSSTLPAGTDDGIEIARVARTML
                     DGIDVSGGVRLLGVGVSGLAEAEHRAEQLSLLDGAGAAPPDGAASPTVDRRRLNEAVD
                     EIRDRFGRASIGPATLARPDVGAFVEGQQQWGPGAQQP"
     gene            complement(1955..2464)
                     /locus_tag="PKY13_05850"
     CDS             complement(1955..2464)
                     /locus_tag="PKY13_05850"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT79489.1"
                     /translation="MDSASVREWLVAGAVIETDEGVLLVRNLRRNGASDWTPPGGVIE
                     ATERADIREGLAREVLEETGLRVTEWGPLLYEVVAEAPDLGWVMRAQVWRVDATDGAL
                     AVGNDPDGIVTDAAYVPLHRCGEHLESTHDWVREPLTDWLDGRFDHQPTYRYRLEDAV
                     AGRGRIVRL"
     gene            complement(2502..4046)
                     /locus_tag="PKY13_05855"
     CDS             complement(2502..4046)
                     /locus_tag="PKY13_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOT79490.1"
                     /translation="MTEIETPSGRVAGRVVDDDTSTVHEFTGIRYGLPPVGPLRLRPP
                     VAVPPWTGVFDATSTPPRSIQPATMLAPAGDPEDEDCLALNVWTPDPTGSLPVMVWMH
                     GGSFTSGSGSIAWYQGARLARRGVVVVTINYRLGALGFLDLAPLGGDDWVGCTNVGLQ
                     DQTLALTWVRDHIAAFGGDPSRVTIFGESAGAMSVSTHLAVPASSGLFRRVIAQSGAA
                     GHVQDTESGRRAATRALDLLGVGPSTLARLADLPTAAFRDVTNTMQNEDPDRDVPLPF
                     RPTVDGSVLPVAPLDALASGAASHIDLLAGTNRDEMNLFRLMALLDGAAPDLEDTRLL
                     RRLDRALARLGRHAGAETALAAYRAHHPDAGNGDLWAAVSTDTVFRMPMLAMLDAHVT
                     GGGSAWSYHFTHPSSGFGGALGAAHAVEIPFVFDNLGQPGTEMMLGDVTPERQRFAAD
                     VADRWATFATADDLTAGSPGWTVPGTDQAWPAYEPDRRAQVQLDVDAAVIEAHQDDLR
                     ELWLGA"
     gene            complement(4087..5382)
                     /locus_tag="PKY13_05860"
     CDS             complement(4087..5382)
                     /locus_tag="PKY13_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT79491.1"
                     /translation="MKRTLVALLILSSLAVGCGDDSKSADDGDSTATTEAAAEGQRTL
                     VVPDDYDTIQAAVDATELGDLVYIKNGTYTEGTVGVTVETENIVIRGEDRNKVIIDGE
                     FKRDNGIKVFSNGVAIENLTVRNNNGNGIFFTGEYNDDTSKNKILTGYRASYITAYNN
                     GLYGVYAFNATKGQFDHSYGSGHPDSAFYIGQCNPCDALLTDNESELNMLGYSGTNST
                     GVTIVNSYFHDNRSGIDPNSLDSEKLAPNSGTIMIGNRVIDNNNVEAPNNEGFQEAYG
                     NGIVLGGVSNNVVERNLITGHILSGVLVTDLPDGYKPEGNKVRNNTLSGNKFDLAYLT
                     VNFPSNLFGNCFEGNTFTTQFPDGLQDKAACGAPEVDLGDLSSVIASVPPTPPDVDWK
                     AVKAPSAQENMPDAKNAKAKPATDVPAKVDLDAITTPTA"
     gene            complement(5462..5911)
                     /locus_tag="PKY13_05865"
     CDS             complement(5462..5911)
                     /locus_tag="PKY13_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HOT79492.1"
                     /translation="MLRRRPLRTPAVAVLAVATLLTAGCGGSDDAADARPSSPTTTFA
                     FPQDELVDETGKAVVEISAIDNSLEPRYVEITVGTRVVWKNEGRNPHDILPAVDGAFA
                     GAVASEMPPGSTHTVTFDTAGDFPYYCSTHGTMKNGMNGAIRVVAAS"
     gene            complement(5945..6880)
                     /locus_tag="PKY13_05870"
     CDS             complement(5945..6880)
                     /locus_tag="PKY13_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT79493.1"
                     /translation="MHEVAGDQTMSSQGLNDWERGSMIPPRRLGALLSDARAKQGNTL
                     EDLAERSNGRFSLAALSSIERGSRDVSDEDARKLATIYGLESTSLVPPRSKLIVDLSE
                     GLLTVDDRSLRVGRSSPSRDEVLARYLTLVYSMRRLEPGAPLTLRVDDLDVLGRALRV
                     GSRTLETDLESLMAHPEQLLGWRSRLLKRRVLIPAAGVMVALLGVGALVLVQGPQSAE
                     AGTNPSVAVPAATATAPVPVDIGSAVVQERNADGTPGPVVVRNGVDAPVSSTSSEVGL
                     IAPETTVAETSVGDDSGAVSVERTPEAAAQDVAVG"
     gene            7083..7376
                     /locus_tag="PKY13_05875"
     CDS             7083..7376
                     /locus_tag="PKY13_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79494.1"
                     /translation="MSEPQSALPSVRARVIAFASILICGALGGAIGYWFADLSDESTL
                     LSGFLMLGGTVVFSLGAAVVSVLSLRALAEWTSSDAARGDELTPVEQLLGKGR"
     gene            7399..8232
                     /locus_tag="PKY13_05880"
     CDS             7399..8232
                     /locus_tag="PKY13_05880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT79495.1"
                     /translation="MPPDVQPLTAGDLPADPAELADLALRTAVAAADMVRAERRAGFG
                     VETKSTDTDMVTSLDKASDVLIRRLLLDARPDDGLLTEEDAEHAGSTGIVWIVDPIDG
                     TTNFVYGHPPYAVSIGAAVGGRPIAGAIVEISADDRYHAWLGGGSHRNGAQLRLGEPP
                     ALAQALISTGFAYDPRRREAQAAVVARIVGQVRDIRRLGAAAYDLCSVAVGRIDGYYE
                     HGLNAWDLAAGEIIAAEAGATVEHLRGGPALPLDGIIAAHPALVGPLRDLLLAHGAGD
                     L"
     gene            8348..>8970
                     /locus_tag="PKY13_05885"
     CDS             8348..>8970
                     /locus_tag="PKY13_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT79496.1"
                     /translation="MGTRILTVEDDERIRTSVKLALEDEGWEVAEATNGEEAIAYFAR
                     QPTDVVLIDIMLPGLDGFDVCRAIRRLSDVPIVMVTARADTHDVVAGLEAGADDYLTK
                     PFVPKELSARIRALLRRARSADTGVTRMRFADLEIVPQEGTVTRNGEEIHLTKTEFRL
                     LVELAANAGRVFSREALLEKVWGHDYFGDGRLVDVHIRRLRTKVEADP"
     assembly_gap    8971..9070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9071..10462
                     /locus_tag="PKY13_05890"
     CDS             <9071..10462
                     /locus_tag="PKY13_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT79497.1"
                     /translation="SNLLDQRERLVQERTLINGATLADKLTSPDIDAQTLFGSLSTAG
                     KPSALVPVTEGSDGDLRSISLDARYGTSALPRALRVQVIVDHQAGIMRYERDGETLLA
                     VGVPIAGGQGGYFEINQIDDIGRAIDSLGYRLVGACLVAALLGGVLGFWASRRVLRPL
                     ADVSQVAEAISLGKFDARLVDTEWADDPDLGQLVSSFNAMVSSMQMRIDRDAQFASDV
                     SHELRSPLTTFNASLEVLRNAREEMPDRAQIALDLLSSDMERFTQLVEDLLEISRFDA
                     GAVRLELDEVLLVETIKQAVRSLSRDPIPVEGSGLDDLLISCDKRRLMRILANFIDNA
                     KKYANSATGITVERVSASTDDPTSGDEEPRLDTVQIAVEDAGPGVPEAERAKIFDRFN
                     RGGQGGSRGTDLGVGLGLALAAEHARLHGGSVWVEGRSDGLPGSRFVVELPLVEPADD
                     PAERSLDEAEAMV"
     gene            10459..11130
                     /locus_tag="PKY13_05895"
     CDS             10459..11130
                     /locus_tag="PKY13_05895"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOT79498.1"
                     /translation="MTVRLRRTAALLVAAGTLVVGACGVPLDSSPRAVIATSSTTEAT
                     TDQANTEAGDTAAFLFFIANNQLINVAQEVPSRSVDDALGALFEGVPPSAADDVVSQI
                     PTGTRLLGVTRIGTLLDVDVSKEFDNLVGTGRSQATAQIVMTATDIAGIDQVSLRIEG
                     QPTQVFSQIRGDTDQVGACDYLGLMPPDDLVQSWALDRRSKQHLTTRHSMLSTQCTAP
                     PAADN"
     gene            11256..12149
                     /locus_tag="PKY13_05900"
     CDS             11256..12149
                     /locus_tag="PKY13_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT79499.1"
                     /translation="MVRPSGASDSGRLSDDPGNSGPHAERSARGPSWTGDTLRESHGP
                     RDRRGLWVGAALAAELRSRQATVLTTDIEGEFDHRLDVRHQDDWQRVLSDTGVPDFLF
                     ANAGISMGGPTQELTRAHWDRIIDVNLNGVVNGLLEVYPAMVERGSGHIVITASGAGL
                     AAPPFVTAYAATKHAVVGLGLGLRSEAALHGVRVSILCPGAVDTPILDRSPPSDLPNT
                     RSKPVTARQYLSLLNQKPVSADRFARLALDRIARDQAIICVPVAAKSLWYLHRLSPSL
                     VARVSKLIAQRVDHHLLGGGN"
     gene            12272..>12495
                     /locus_tag="PKY13_05905"
     CDS             12272..>12495
                     /locus_tag="PKY13_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT79500.1"
                     /translation="MSLVLSIDAGTTGVRAFAVDESGATRGWSYREFTQHFPQPGWVE
                     HDATEIWEAVQTTTAELVAALDEPIAAIGIT"
BASE COUNT         1844 a         4330 c         4320 g         1901 t
ORIGIN      
        1 tcggcaacca cgtcctcggc gccgccgtgg cgcagatcgg cgcgtggaac gaggagttcg
       61 ggcccggcga cttctgggtg tcgtgcaacg tcgccccgcc ccagctcgac gacagcacct
      121 tccccgaccg ggtgatgcgg atcctcgacg gtgcgccggt cgcggcgcac cggctgtgcg
      181 tcgaggtgac cgaggactcg atgatgtcga ccgaccgttc gaaggagacc ctcgaccggc
      241 tccggcggtc gggggtgcgc atcgccctcg acgatttcgg caccggctac tcgtcgctcg
      301 tgcacctcat cgacgtcaac ttcgacatcc tcaagctggc cggcgacctg atgcccccgc
      361 accggggcta cggcgccgcg accgccaacg gtcgccagat cctcggggcc gtcgtgaaca
      421 tggcccactc gatcggcctc gacgtcgttg ccgaggccgt cgagaccgag gtccagtggg
      481 acgagctggc gtcgtgtgga gccgatctcg tccagggatg ggccttctcg aagtcacgcc
      541 cgccgggtga tcccttcctc gacgccctcg tctcgggagc gtcggcacgc gtcggcgccg
      601 tcgtctgacc gctcatcgcc ggtgatcggg gcggccgacg cgaccgatcc cgcctccggc
      661 gttcctcagg gttgctgggc gcccgggccc cactgctgtt ggccctcgac gaacgcaccg
      721 acgtcgggcc gagccagggt ggccgggccg atcgacgccc gaccgaaccg gtcgcggatc
      781 tcgtccacgg cctcgttgag gcgacgtcgg tcgaccgtcg gcgacgccgc cccatcgggc
      841 ggggccgctc cggcaccgtc gagcaacgag agctgctcgg cccggtgctc tgcttcggcg
      901 agaccgctca ccccgacgcc gagcaggcgc acgccaccgg acacgtcgat gccgtcgagc
      961 atcgttcggg cgacccgggc gatctcgatc ccgtcgtcgg tgccggccgg cagggtcgag
     1021 gaacgggtca acgtctggaa cgtgccgtgc cgcagcttga gctgcacggt gcgcgcgtag
     1081 ctcccggccc gtcggagccg gtcggcgacc gcgtcggcca ggcgcaccac ttcacgttcg
     1141 agatgggccc gatcggtgac gtcggtcggg aacgtctctt cgtggctgat cgatttcgcg
     1201 acctggccgg gttcgacggg tcggtcgtcg aggccgtggg acaaccggtg cagctgggtg
     1261 ccggacgcgg tgccgacggc ccgacagagt gtgtcgagcg gcagccgggc gatgtcgccg
     1321 acggtcgcga cgccgagtcg ttccagcttc gcgagcgtcg cagggccgac gccccacagg
     1381 tcggacgcgg cgagcggcca gaggaacgcc tcgacgtcgt cgggatcgac gacggcgaca
     1441 ccgctgccga acactggccc ggtcggagtc gcccgaggct tggcggtctc ggtggccaac
     1501 ttggcgagga acttgttgtg cgccacgccg accgagcagg tgagcccgac ctcgtcgagg
     1561 actcgccgtc ggatcgtggc cgcgataccg ggcgccggac cgagggcacg cacccggcca
     1621 ctcacgtcga ggaacgcctc atcgagggac aacggctcga ccaacggtgt gatcgacccg
     1681 aagatgtcca tcaccacggc gctgacctcc cggtagcgct cgtgacgtcc atcgaggaac
     1741 agggcgtggg ggcacaggcg tcgggcgacc gcactcggca tcgccgaatg cacaccgaac
     1801 gcccgggcct cgtaggacgc cgccgccacc accccgcgtg gaccggtgcc gccgacgacg
     1861 acggggcgac cgcgcagctc ggggcgttcg aggagttcga ccgacacgaa gaacgcgtcc
     1921 atgtcgacat ggaggatgtg ccggcgggcg gcgctcacag tcggacgatt cggcctcgcc
     1981 cggcgacagc gtcctcgagc cggtaccggt aggtcggctg gtggtcgaac cgaccgtcga
     2041 gccaatcggt cagcggctca cggacccagt cgtgggtgct ctccaggtgt tcgccgcacc
     2101 gatggagcgg cacgtaggcg gcgtcggtga cgatgccgtc ggggtcgttg ccgacggcga
     2161 gtgccccgtc ggtggcgtcg actcgccaca cctgcgcccg catgacccac ccgaggtcgg
     2221 gcgcctcggc gaccacctcg tacaggagtg gcccccactc ggtgactcgg aggccggtct
     2281 cctcgaggac ctcgcgagcg aggccctctc ggatgtcggc gcgctcggtc gcctcgatca
     2341 cgccgcccgg cggtgtccag tccgaggcac cgttgcggcg caggttccgc acgaggagga
     2401 caccctcgtc cgtctcgatc acggctccgg cgacgagcca ctccctcacg cttgcgctgt
     2461 ccacgggcac agtctctccc aaccgggcac ggtcgtgcgg ctcaggcgcc gagccacagc
     2521 tcgcggaggt cgtcctggtg cgcctcgatg accgctgcgt cgacgtcgag ctgcacctgg
     2581 gcacggcggt cgggttcata cgcgggccat gcctgatcgg tgccgggcac cgtccacccg
     2641 ggcgaaccgg ccgtgaggtc gtcggccgtg gcgaacgtgg cccaccgatc ggccacgtca
     2701 gcggcgaatc gttgccgttc gggtgtgacg tcgccgagca tcatctcggt gccgggctga
     2761 cccaggttgt cgaacacgaa cgggatctcc acggcgtgcg ccgcgccgag cgcgccgccg
     2821 aaccccgacg agggatgcgt gaagtggtac gaccacgccg acccgccccc ggtgacgtgc
     2881 gcgtcgagca tggcgagcat cggcatgcgg aagaccgtgt cggtggacac cgcggcccac
     2941 aggtcgccgt tgccggcgtc ggggtggtgg gcccgatacg ccgcgagggc ggtctcggca
     3001 ccggcgtggc gtccgagtcg cgccaacgcc cggtcgaggc ggcggagcag ccgggtgtcc
     3061 tcgagatcgg gcgcggcgcc gtcgaggagc gccatgaggc ggaacaggtt catctcgtcg
     3121 cggttcgtgc ccgccagcag gtcgatgtga gacgcggcgc ccgaggcgag ggcgtcgagc
     3181 ggcgcgaccg ggagcacaga gccgtcgacg gtcggccgga acggcagcgg gacgtcgcgg
     3241 tcggggtcct cgttctgcat ggtgttggtg acgtcgcgga aggccgcggt cggcagatcg
     3301 gccagccggg cgagcgtcga cgggccgacg ccgagcaggt cgagggcccg cgtcgcagcg
     3361 cggcgacccg actcggtgtc ctgcacgtga ccggccgcgc cggattgcgc aatgacccgc
     3421 cggaacagac ccgacgaggc cgggaccgcc aggtgggtcg acacgctcat ggccccggcg
     3481 ctctcgccga agatcgtcac ccgggaagga tcaccgccga atgcggcgat gtgatcgcgc
     3541 acccacgtca aggccaaggt ctggtcctgg agcccgacgt tggtgcaccc gacccagtca
     3601 tcgccgccga gcggggcgag gtcgaggaac ccgagggccc ccaaccggta gttgatggtg
     3661 acgaccacca ccccacgtcg agccaggcgg gcaccctggt accaggcgat cgagccggac
     3721 cccgacgtga agctgccccc gtgcatccac accatcacag ggagcgagcc ggtgggatcg
     3781 ggtgtccaca cattgagcgc gaggcagtcc tcgtcctcgg ggtcgccagc gggagcgagc
     3841 atcgtcgccg gttggatgct ccgtggcggc gtcgaggtcg cgtcgaacac accggtccac
     3901 ggcggcaccg ccaccggcgg gcgcaaccgg agcggcccga ccggcggcag gccgtaccgg
     3961 atgcccgtga actcgtgcac ggtggacgtg tcgtcgtcga cgacgcgtcc ggcgacgcgg
     4021 cccgacgggg tctcgatctc ggtcaacgtc gaggaccccg gtcgcggccc gagtgctccc
     4081 ccgtcgtcac gccgtcggcg tggtgatggc gtcgaggtcg accttggccg gcacgtcggt
     4141 cgccggcttg gccttggcgt tcttcgcgtc gggcatgttc tcctgcgccg acggcgcctt
     4201 gacggccttc caatcgacgt cgggcggggt cgggggcacg ctggcgatca cgctgctgag
     4261 atcgccgagg tcgacctcgg gggcgccgca cgccgccttg tcctggagcc cgtcggggaa
     4321 ctgcgtcgtg aacgtgttgc cctcgaagca gttgccgaag aggttcgacg ggaagttcac
     4381 ggtgaggtac gcaaggtcga acttgttgcc cgacagcgtg ttgttgcgca ccttgttgcc
     4441 ctcgggcttg tacccgtcgg ggagatcggt gacgagcaca cccgagagga tgtgcccggt
     4501 gatgaggttg cgctcgacca cgttgttcga cacgccaccg agcacgatgc cgttgccgta
     4561 ggcctcctgg aagccttcgt tgttgggtgc ctccacgttg ttgttgtcga tgacccggtt
     4621 gccgatcatg atcgtcccgg agttgggcgc gagcttctcg ctgtcgagcg agttggggtc
     4681 gatgccgctg cggttgtcgt ggaagtacga gttgacgatc gtgacgcccg tggagttcgt
     4741 gcccgagtag ccgagcatgt tgagctcgga ctcgttgtcg gtcaggagcg catcgcaggg
     4801 gttgcactgg ccgatgtaga acgccgagtc ggggtggccc gaaccgtagg agtggtcgaa
     4861 ctgccccttg gtggcgttga aggcgtagac gccgtacaag ccgttgttgt aggccgtgat
     4921 gtagctggcc cggtagcccg tgaggatctt gttcttcgac gtgtcgtcgt tgtactcgcc
     4981 cgtgaagaag atgccgttgc cgttgttgtt gcgcaccgtc aggttctcga tggccacgcc
     5041 gttggagaac accttgatgc cgttgtcgcg cttgaactcg ccgtcgatga tgaccttgtt
     5101 gcggtcctcg cctcgaatga cgatgttctc ggtctccacg gtcacgccga ccgtgccctc
     5161 ggtgtaggtg ccgttcttga tgtacacgag gtcgccgagt tcggtcgcgt cgacggcggc
     5221 ctggatggtg tcgtagtcgt cggggacgac gagtgtgcgc tggccctcgg cggccgcctc
     5281 ggtggtcgcg gtggagtcgc cgtcgtcggc cgacttcgag tcgtcgccgc agccgacggc
     5341 gaggctggac aggatcagca gtgcaaccag tgtgcgtttc atctgcggca ccctagtgac
     5401 cgctgtcacg tcctgacggc aatctcgggg gatgccgagg ggccgcccgg cccggtcgaa
     5461 cctaggaggc ggcgacgacc cggatcgcgc cgttcatgcc gttcttcatc gtgccgtgcg
     5521 tcgagcagta gtacgggaag tcgccggcgg tgtcgaacgt gacggtgtgg gtcgaaccgg
     5581 gcggcatctc cgacgccacg gcaccggcga acgcaccgtc gacggcgggg aggatgtcgt
     5641 gggggttgcg cccctcgttc ttccacacca cgcgggtccc gacggtgatc tcgacgtacc
     5701 gcggctcgag gctgttgtcg atcgcgctga tctccaccac cgccttgccg gtctcgtcca
     5761 cgagttcgtc ctgggggaac gcgaaggtgg tcgtcgggga cgacggtcgt gcgtcggcag
     5821 cgtcgtcgga gccgccacac cccgcggtca gcaatgtggc aacggcgaga acggcaacgg
     5881 cgggggttcg gagggggcgg cggcgaagca tcgggcgatt gtaggcagcc gtccgccacg
     5941 acgttcagcc gacggcgacg tcctgcgctg ccgcttcggg ggtgcgctcg accgacacgg
     6001 caccgctgtc gtcgccgaca ctcgtctcgg cgacggtcgt ctccggtgcg atgagcccga
     6061 cctcggagct cgtcgacgag accggggcat cgacgccgtt gcgcacgacg accgggccgg
     6121 gggttccgtc ggcgttgcgc tcctgcacga cggcggagcc gatgtcgacg gggaccggcg
     6181 ccgtggccgt ggcggcaggg acggccacgg acgggttggt gcccgcctcg gcggactgcg
     6241 ggccctgcac gaggacgagg gcgccgacgc cgaggagcgc gaccatcaca ccggcggcgg
     6301 ggatgagcac gcggcgcttg agcagacgcg agcgccatcc gaggagctgc tcggggtggg
     6361 ccatgagcga ctcgagatcg gtttcgagcg tccggctgcc gacgcggagc gctcgaccca
     6421 ggacgtcgag gtcgtcgacg cggagggtga gcggagcgcc cggctcgagc cggcgcatgg
     6481 agtagacgag ggtgaggtag cgggcgagca cctcgtcacg tgacggcgag gagcggccga
     6541 cgcgaaggct gcggtcgtcg acggtgagca gcccctcgct caggtcgacg atcagcttcg
     6601 aacgcggcgg gacgagcgac gtggactcga gtccgtagat cgtggcgagc ttccgggcgt
     6661 cctcgtcgga cacatcgcgc gaaccccgct cgatcgagct gagggctgcg agggagaatc
     6721 gaccgttcga gcgttcggcc aggtcttcga gcgtgttgcc ctgcttggcg cgggcgtcac
     6781 tcagcagcgc gccgaggcga cgcggcggga tcatgctgcc ccgctcccag tcgttcagtc
     6841 cctgcgaact catggtctgg tctccggcta cctcgtgcat ggcctgtctc ctcgtcgatc
     6901 gccgtgggcg gtcggtcacc agttcggtgc ggcagtcacc ttctgcggcg acgctgccac
     6961 ttacagtaaa gatctggcga tcgaacacca gaaggcgttg gacccatccc actgggtaga
     7021 tcggcccgct ctcccacccc cttgagcaat tcgcagcggc aacggcgggt acgctcacga
     7081 cgatgtcgga accccagagc gccctcccat cggtgcgagc ccgcgtgatc gcgttcgcgt
     7141 cgatcctcat ctgcggagcg ctcgggggcg ccatcggcta ctggttcgcc gacctgtccg
     7201 acgagagcac gctgctctcg gggttcctca tgttgggagg cacggtcgtg ttctcgctcg
     7261 gcgccgcggt cgtctcggtg ctgagcctgc gggcgctcgc cgaatggacg tcgagcgacg
     7321 ccgcccgggg cgacgagctc acgccggtcg agcagctgct gggcaagggc cggtagcacc
     7381 aaccggtagg ttggccacgt gccacccgat gttcagcccc tcacggccgg cgatctgccc
     7441 gccgatcctg ccgagctcgc cgacctcgct ctccggaccg ccgtcgccgc ggccgacatg
     7501 gtgcgcgccg aacgccgcgc cgggttcggc gtcgagacca agtcgaccga caccgacatg
     7561 gtcacctcgc tcgacaaggc gtccgacgtc ctcatccgtc gcctgctgct cgacgcccgt
     7621 cccgacgacg gcctgctgac cgaagaggac gccgagcacg ccgggtccac cggcatcgtg
     7681 tggatcgtcg acccgatcga cggcaccacc aacttcgtct acggccaccc gccctacgcc
     7741 gtcagcatcg gtgccgcggt cggcgggcgc ccgatcgccg gcgcgatcgt cgagatcagc
     7801 gccgacgacc gctaccacgc ctggctcggc ggcggctccc accgcaacgg ggcccaactc
     7861 cgcctcggtg aaccgccggc gttggcacag gccctcatct cgaccggctt cgcctacgac
     7921 ccccgtcgac gcgaggcgca ggcggcagtc gtggcccgca tcgtcggcca ggtccgggac
     7981 atccgtcgcc tcggcgccgc cgcgtacgac ctgtgctcgg tcgccgtcgg ccgcatcgac
     8041 ggctactacg agcacgggct caacgcctgg gatctggccg ccggcgagat catcgccgcc
     8101 gaggccggcg ccacggtgga gcacctgcgt ggcggtccgg cactccccct cgacggcatc
     8161 atcgccgcgc accccgccct cgtgggcccg ctgcgcgacc tgttgctggc gcacggcgcc
     8221 ggcgacctct gacccgccgt cagcgaccac ccgacggcgg agcggacctg acccgagcgg
     8281 gggaacaacc gggtcgcccg caccgagaga cccgatcgag cggctgcggc gtgcgagact
     8341 cacgggcgtg ggcacacgaa tcttgacggt cgaggacgac gaacgaatcc gcacctcggt
     8401 gaagttggcg ctcgaggacg agggctggga ggtcgccgag gcgaccaacg gtgaggaggc
     8461 catcgcgtac ttcgcccggc agcccaccga cgtcgtcctc atcgacatca tgctgcccgg
     8521 cctcgacgga ttcgacgtgt gccgcgccat ccgccggctg agtgacgtgc cgatcgtcat
     8581 ggtcaccgcc cgcgccgaca cccacgacgt ggtcgccggg ctcgaagcgg gcgccgacga
     8641 ctacctcacc aagccattcg tcccgaagga gctgtcggca cggatccggg cgctgctccg
     8701 acgggcccgg tcggccgaca ccggggtgac ccgcatgcgg ttcgccgacc tcgagatcgt
     8761 cccccaggag ggcacggtca cccgcaacgg cgaggagatc cacctcacca agaccgagtt
     8821 ccgcctgctc gtggaactcg cagcgaacgc cggccgcgtg ttcagtcgcg aagcgctcct
     8881 cgagaaggtg tggggccacg actacttcgg cgacggtcga ctcgtcgacg tccacatccg
     8941 gcgcctccgc accaaggtcg aggccgaccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn tccaacctgc tcgaccagcg cgagcgactc gtccaggaac gcaccctcat
     9121 caacggggcg acgctcgccg acaagttgac gtcgcccgac atcgacgccc agacgctgtt
     9181 cggctcgctc tcgaccgcag gcaagccgtc ggcgctcgtc cccgtgaccg aggggtccga
     9241 cggcgacctc cgctcgatca gcctcgacgc ccgctacggc acgagcgccc tcccccgcgc
     9301 cctgcgtgtc caggtcatcg tcgatcacca ggccggcatc atgcgctacg agcgtgacgg
     9361 cgagacgttg ctcgccgtcg gcgtgccgat cgccgggggc cagggcggct acttcgagat
     9421 caaccagatc gacgacatcg gtcgggcgat cgacagcctc ggctaccgcc tcgtcggcgc
     9481 gtgcctggtg gccgcgctgc tcggcggagt gctcgggttc tgggcgagcc ggcgggtgct
     9541 ccgcccgctc gccgatgtct cccaggtcgc cgaggccatc tcgctcggca agttcgacgc
     9601 ccgtctcgtc gacaccgaat gggccgacga ccccgacctc ggccagctcg tctcgtcgtt
     9661 caacgcgatg gtgtcgtcga tgcagatgcg catcgaccgc gacgcgcagt tcgcctccga
     9721 cgtcagccac gagctgcgct cccccctgac cacgttcaac gcgtcactcg aggtgctgcg
     9781 caacgcccgc gaggagatgc ccgaccgggc gcagatcgcc ttggatctgc tgtcgagcga
     9841 catggagcgg ttcacgcagc tggtcgagga cctgctcgag atcagtcgtt tcgatgccgg
     9901 agcggtgcgg ctcgagctcg acgaggtgct gctggtcgag acgatcaagc aggcggtgcg
     9961 gtcgctctca cgcgatccga tccccgtcga ggggtccggc ctcgacgacc tcctcatctc
    10021 gtgcgacaag cgacgtctga tgcgcatcct cgcgaacttc atcgacaacg ccaagaagta
    10081 cgccaacagc gccaccggca tcaccgtcga gcgggtgtcg gcatcgaccg acgacccgac
    10141 gtccggcgac gaggagcccc gcctcgacac ggtgcagatc gcggtcgagg acgccggacc
    10201 gggtgtgccc gaggcggagc gggcgaagat cttcgaccgg ttcaaccgag gcggccaggg
    10261 tggcagccgt ggcaccgacc tcggggtcgg cctgggcctc gcgctggccg ccgagcacgc
    10321 ccgcctccac ggaggctcgg tgtgggtcga gggccggtcc gacggtctgc ccggctcccg
    10381 cttcgtcgtc gaactgccgt tggtggagcc cgccgacgat ccggcggaac gatccctcga
    10441 cgaggcggag gcgatggtat gaccgtccgg ctgcgccgaa ccgctgccct cctggtggcg
    10501 gccggaacgc tggtcgtcgg cgcctgcggc gtgccgctcg acagctcgcc ccgggcggtg
    10561 atcgccacct ccagcaccac cgaggcgacg accgaccagg ccaacaccga agcgggcgac
    10621 accgcggcgt tcctgttctt catcgccaac aaccagctga tcaacgtcgc ccaagaggtg
    10681 ccgagccgct cggtcgacga cgcactcggc gcgctgttcg agggcgttcc gccgagcgcc
    10741 gccgacgacg tcgtctcaca gatcccgacg ggcacgcgcc tgttgggcgt cacccgaatc
    10801 ggaacgctcc tcgatgtcga cgtgtcgaag gagttcgaca acctcgtcgg caccggccgc
    10861 tcacaggcca ccgcccagat cgtcatgacc gcgaccgaca tcgccggcat cgaccaggtg
    10921 tcgctccgca tcgaggggca gcccacccag gtgttctccc agatccgcgg cgacaccgac
    10981 caggtcgggg cctgcgacta cctgggcctc atgccgcccg acgatctggt gcagtcatgg
    11041 gcactcgatc gccggtcgaa gcagcatctg acgacgcgcc acagcatgct ctcgacgcag
    11101 tgcaccgcac ccccggccgc tgacaactga ccactgtgtc ggccccggcg gagccgacac
    11161 agtgcgcgtt ccgtaatcca tgcgaaaagt gacggccggc gacacatctc gcatggattt
    11221 cgtccgctcg tcgacaccct cgggtgggga accctgtggt ccgacccagc ggagcgtcgg
    11281 acagcggacg actcagcgac gaccccggaa actcaggccc acacgccgag aggtcggcgc
    11341 gtgggccaag ctggacgggt gacacccttc gcgaatccca cggccctcgt gaccggaggg
    11401 gcctctgggt cggcgctgcg cttgctgccg agctcaggtc gcggcaagcc acggtgctca
    11461 cgaccgacat cgaaggcgaa ttcgatcacc gtctcgatgt ccgacaccag gacgactggc
    11521 agcgagtcct cagcgatacc ggcgtacccg acttcctctt cgccaacgcg ggaatctcga
    11581 tgggcggacc gacgcaggag ctgacccgcg cccactggga tcgcatcatc gatgtgaacc
    11641 tcaacggagt ggtcaacgga ctactggagg tctacccggc catggtcgag cgcgggtcgg
    11701 gccacattgt catcaccgcc tccggtgcgg gtctcgctgc gccacccttc gtcactgcgt
    11761 acgccgcgac gaagcacgcc gtcgtcggct tgggtctggg ccttcgatcc gaagcggccc
    11821 tccacggcgt acgcgtgagc attctgtgcc cgggcgctgt cgatacaccg attttggacc
    11881 gctcacctcc cagcgacctc cccaacaccc gatccaaacc tgtcactgcg cggcagtatc
    11941 tgtcgctgtt gaatcagaag ccggtttcgg ccgaccgttt cgcaaggctg gcgttggatc
    12001 gaatcgcccg tgaccaggcg atcatctgtg tgccggtcgc tgcgaagtcg ctttggtact
    12061 tgcatcggct ttcgccgtcg ttggtggccc gggtctcgaa gctcatcgca cagcgggttg
    12121 atcaccacct cctcggcggt ggcaactgag ccggctcgtc cgcggccgat gtgacatcac
    12181 ccggtagcaa gtcggcccgg actggtggca ggtcactacg accgcgccgc cagcgggtcg
    12241 actgcccggg tcgccggtag attcggactc catgtcactc gtgttgtcca tcgacgccgg
    12301 aaccaccggt gtccgagcgt tcgctgtcga cgagtccggg gcgacccggg gctggtcgta
    12361 ccgcgagttc acccagcact tcccgcagcc gggctgggtc gagcacgacg ccaccgagat
    12421 ctgggaggcc gtgcagacca cgaccgccga gctggtggcc gccctcgacg agccgatcgc
    12481 cgcaatcggc atcac
//
LOCUS       DAOSOA010000062        12352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5115_length_12352_cov_5.968773, whole genome shotgun sequence.
ACCESSION   DAOSOA010000062 DAOSOA010000000
VERSION     DAOSOA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12352
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5115_length_12352_cov_5.968773"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKY13_05910"
     CDS             complement(<1..226)
                     /locus_tag="PKY13_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT79501.1"
                     /translation="MGRKILFVTTDQQRYDTLGCNGGTLARTPVIDGLAAQGVRYERA
                     VPQSVVCMPSRSTMLTGQHPSTHGVWMNGVP"
     gene            complement(219..674)
                     /locus_tag="PKY13_05915"
     CDS             complement(219..674)
                     /locus_tag="PKY13_05915"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT79502.1"
                     /translation="MATIRREIRIAASADDIWKVVGRPDLLHHWFPGMSDCTVQTVDG
                     VTTRTIVVGAGIPMPEEILINDPIQRRFQYRLTVPAFEFHRGTIDVIELAPDDTLVVY
                     STDCIPNAMALVIGGATGGALDELKAQFDAGEGPALEAAGVTLKETARG"
     gene            complement(698..2305)
                     /locus_tag="PKY13_05920"
     CDS             complement(698..2305)
                     /locus_tag="PKY13_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT79503.1"
                     /translation="MARNILFVTTDQQRFDSLGCNGGTIARTPVADALARDGINYQRA
                     YNNNTVCMPARCTMLTGQYPRTHGVVANGIALPTDAPSIAAHLKTAGYHTALVGKAHF
                     EPAFDMEGRWEENLRWRRNDTGPWRGFDYSIQAAHVPSFQGRPVQHYGRWLKAEHPEH
                     LDSFCELLNAAPGGDTGAPETKHNPIPTDWYHTDWTADRTIDWLDTVPADDPFFCWMS
                     FPDPHHPWDPPESEMGRVNWRDLDLPPGHPGSPEKIREVLGGKPAHWLGWYDGTFANA
                     EGGPMYFFPASLTDDQVREINARTHVMVELLDEALGRVLAHLDAIGRLADTDIVFTTD
                     HGEMQGDMGFIFKGPWHVDSLMRLPLIWKPAASAGIDAAVVTDPVGQVDLAPTFCAIA
                     GVEPAPWMQGSRLPTFDGEATTDSFGQTAVHERALTEWDSQFPGYGMHLRSIVRDGWM
                     CTAYEESTDGQPNGLEKVWGDSVLRPCGVHYDGTEGELYNLVEDPHQWHNLWDDPAYR
                     AQRDDLVADMVASFPTERRVLPVEAPA"
     gene            2395..3018
                     /locus_tag="PKY13_05925"
     CDS             2395..3018
                     /locus_tag="PKY13_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT79504.1"
                     /translation="MGLPDHVLDDSALDATVIDGRHQRSARSRDAVVEALLSLYEDGH
                     LRPGVALIAERAGVSQSSVFRHFQDVDELVRSAIDHQWERLRELFTRPLVGGSLDERI
                     AALVAQRIRLYDIAGPALRAARLLAPDSAAVRSAFALRRQLLRGQVEDLFAAELAALP
                     SSRRRTTLDALDVVTGFEAVEALRIDRGAGIRRTTDVLTLTIKGLLS"
     gene            3015..3773
                     /locus_tag="PKY13_05930"
     CDS             3015..3773
                     /locus_tag="PKY13_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT79505.1"
                     /translation="MTEAPSPDDRRVVVLNDARRYVGPALSRLLAARGHDLVVGEIED
                     SLAEELAALGASVEIVAGGRDLTDPEAAGRLVAAAHERFGRIDAAVSFGGVIVTGSFR
                     RSTPEDLAHAVSGCLEAPYHFLKAVVDPMVEQGVGQVLVITSAAGARPTDGAPLYSSA
                     RAAANMLVRNVAAEVAGKGVQVNAVGTNFMDFPDFLRATGADDPSVRAAVESMVPMHR
                     LGTMEEFASMCLPFIDGTSRFVTGQFIACAGGWA"
     gene            complement(3797..4009)
                     /locus_tag="PKY13_05935"
     CDS             complement(3797..4009)
                     /locus_tag="PKY13_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79506.1"
                     /translation="MTRSTKKKFRLGALAAVLAFSSSACAIPVGNPLNIPLVTGCTLF
                     IAEPGQGAIVVPISAGFLEIGCNLGL"
     gene            4235..5101
                     /locus_tag="PKY13_05940"
     CDS             4235..5101
                     /locus_tag="PKY13_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT79507.1"
                     /translation="MTVITFDVTDDLSGEGGLRIEADAYGDALARPVLLLHGGGQTRH
                     SWGSTAATLAAAGWYAVAYDQRGHGASGRSPSGHYEPGRFAEDLVEIATTFEQPPVVV
                     GASLGGLAALLAEGVLAPGLFAGIVLVDITPRQEREGVNRIVSFMLDRAVEGFGSLDE
                     AADAVAAYQPHRSRPRDHSGLQKNLRLDADGRWRWHWDPELFNSDNGLHAAQEPGRFV
                     AAAAQLTLPTLLVRGKLSDLVSEETAQEFLELVPHAQFVDVSDAGHMVAGDRNDRFCD
                     AVVEFLETLPTN"
     gene            complement(5131..5586)
                     /locus_tag="PKY13_05945"
     CDS             complement(5131..5586)
                     /locus_tag="PKY13_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79508.1"
                     /translation="MTTSPPAPTTLAERPRRLGVGVWDALVVAAGVVLVTSPGLRGQF
                     HAHVRLTPTRWLSAPSAMATLALVALVVTLVGRRREILGLLGLAAVSLGLAAFWLLLG
                     HHQWAGPVLVDLGDDHGIHLGDFLALVPVAAAGALVWTAAIRFRTTRRV"
     gene            5688..7517
                     /locus_tag="PKY13_05950"
     CDS             5688..7517
                     /locus_tag="PKY13_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT79509.1"
                     /translation="MTDQPPPAPEHVDDPWDGVDIDNAVASFFRALADGPLRDDALLA
                     ALRDDGAWGAIGELGDDDFAAEISDLDVPGLWRTQMAMWFDVFRTLDGRVFTHRVTAD
                     ELAEGAVAALPDLTLVDWDVPARRIVGGSGAIATDFDPAFDRFEGGRLIGPDGWLDGF
                     APGDVLGLRLVRDDTVRVERIDAADLPGPDDSAVAELATAFRPCFDRWSNGHGDELAP
                     AVLDLLTDAPDAFRTATLPLGELAIAAGLERRGLEFGPADRPWLTHAQQAFRRRGDEL
                     AADLVDWLASPLRTVVGVMVEWLGDADALPASSVAKARDALATEGVAGMAGELFRGHT
                     APGRLARFVEALGGPGNDAATPSIHVLVGIAAELGGRFDDAEQEFRAALDVDPGQLDA
                     VVELGILASDRGDAVEAERRFRSAAGPEAWLRALSGLLPSRATGRNEPCPCGSGRKFK
                     QCCLVNPTTPVERRGPWYELRLARHLANRDALAFEIFYGVLSEYLPEEAEINDALVDD
                     LVSLATGNVGSYLEAHADRLSDVDRAGLEAWRDAPVTLHHDPESGVTARRVVPLGTAE
                     YVVWSLPLDATAAEELEGLLGDDDAVTSVVEWALWYASTFEED"
     gene            7625..11329
                     /locus_tag="PKY13_05955"
     CDS             7625..11329
                     /locus_tag="PKY13_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT79510.1"
                     /translation="MAHRRNVIVAFGSAIVGQGVAALVPVIQRVVIDDGIVGRTRPVG
                     PWLAALAATAVFVFGCSYLRRWVGGRVSLDVQYDLRVAIFERLQRLDFAGHDELQTGQ
                     LVSRSSSDVGLIQGLLAFLPIIVGNVVLLVVALVVMVVLSPLLTLVMAVMLPLLLVVT
                     LRMRQWIFPATWDAQQRAGVVAGVVDEAVSGVRVVKGFGQEQREIDRLAGAAESLFAS
                     RSRLIRLNARYTPALQAIPSLAQVAVLALGGYLAIHGHITIGTFLAFSSYLVQLVAPV
                     RMLASLFAIGQQARAGAERLLDVLDATAAVTDRPGAVDLPPVRGAIAFEDVTFGYDRS
                     EPVLSGFDLHIEPGEVVAVVGVSGSGKSTVAALVARFYDVGTGRVTIDGVDVRDVTLA
                     SLRGQIGVVFEDAFLFSDSVRDNIAYAVPGATEEQIVVAARAAGAHDFITALPDGYNT
                     LVGERGLTLSGGQRQRVALARAILADSRVLVLDDATSSVDSITEESIHDTLRTIAADR
                     TTILIAHRRSTLQLADRVVVVEDGRVVEEGTDAELTARSERYRRLLAGPDAGDDLGED
                     DPDSASSAAPTDGRNAWPDEPAAVVGLAGAAAAAAKPAIGGPGGGGMNVAFTATPDLL
                     ERLERLPTATDRTGMDVAAAAAPQRGRFRFVGFIRPWRRPLALGFALVALDSGLTLLG
                     PWFVRRGLDQGVDARDTGALWWASAAFAVTVALDWVVTRSSTLVTGRTAERALASLRV
                     RIFAHLQRMSLDYYDAEMDGRIMTRMTTDVEALAQVIQTGVVSAVVAAVTCLGVAVFL
                     VVLSPPLALAAAVVLPFLALATWWYRRRSSVAYGRARDAIADVNADLQENLSGVRVAQ
                     ASVREERNLVRYRRVNQRYLEHRLGAQRLIALYFPLVLLLGDLAAVSVLATGSALVHR
                     GAITTAVVIAFLLYLDQFFSPIQQLSQVLDTWQQATASLVKIEELMSTPTGTPESTHP
                     VEIGRLTGAIALDDVHFRYPTATGDEAVAGVSVRLDAGSTVALVGSTGAGKSTILKLI
                     ARFYDPVSGAVRVDGRRPDGSSAAGVDLRDVGLSSFRRQLGIVPQEAFLFATTVRDNI
                     AYGRPDATDAEVEAAARAVGAHAFVASLPHGYREPISERGRSLSSGQRQLLALARARL
                     VDPPILLLDEATSNLDLHSEARVQRAMRSAAAGRTTVLVAHRLQTARLADRILVIEGG
                     RIVQDGTHDDLLATPGRYRDLWSSYDGAVG"
     gene            complement(11308..12012)
                     /locus_tag="PKY13_05960"
     CDS             complement(11308..12012)
                     /locus_tag="PKY13_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT79511.1"
                     /translation="MRSADVSPWTAVVFDFDGLILDTETPLYDAWRTTFEHHGAEPIP
                     LDEWLSSLGLHDDDPRRLDPFARLQAAVDRDLDSEEVQAARRVLKDTAIAAELLRPGV
                     TGVLDQADELGLPVAVASSSSFEWVGGHLAGRGLLHRFATISCAENGVPGKPAPDTYL
                     RACAAIGVAPTGALALEDSPNGVRAAKAAGLTCVAVPNPISRTLDYSHSDATLTSLEE
                     LDLTLPIARYQPTAPS"
     gene            complement(12070..>12352)
                     /locus_tag="PKY13_05965"
     CDS             complement(12070..>12352)
                     /locus_tag="PKY13_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79512.1"
                     /translation="TTLVKPSVGDTKKPTFTPVKVSLGRQLFTPTWSAAAQFPFNPAT
                     GYDGNDYNLSAGTALTSSLSSQCLGDGKGVLVTGAVSPPRGRWTPGEDD"
BASE COUNT         1651 a         4314 c         4408 g         1979 t
ORIGIN      
        1 gtggcacccc gttcatccac acaccatggg tggacgggtg ctggcccgtg agcatggtcg
       61 aacgcgacgg catgcacacg accgactgcg gcacggcgcg ctcgtagcgg acaccctgtg
      121 ccgccagccc gtcgatcacc ggggtgcggg cgagcgtgcc gccgttgcag cccagcgtgt
      181 cgtaccgctg ctgatcggtg gtcacaaaga ggatcttcct acccacgtgc cgtctccttg
      241 agggtcacgc cggcggcttc gagggcgggg ccctcgccgg cgtcgaactg ggccttgagc
      301 tcgtcgagcg ctcccccggt ggcgccgccg atgacgagcg ccatggcgtt ggggatgcag
      361 tcggtcgagt acacgacgag cgtgtcgtcg ggggcgagct cgatcacgtc gatcgtgccg
      421 cgatggaact cgaaggcggg gacggtcagg cggtactgga agcgccgctg gatcggatcg
      481 ttgatcagga tctcctcggg catcgggatg ccggcaccca ccacgatggt gcgcgtcgtc
      541 accccgtcga ccgtctgcac cgtgcagtcg gacatgccgg ggaaccagtg gtgcaggagg
      601 tcgggtcgcc cgacgacctt ccagatgtcg tcggcggacg ccgcgatgcg gatctcccgt
      661 cggatggtgg ccatcggtgg gttcctctcc tgccgcgtta cgccggcgct tcgacgggca
      721 gcacccgtcg ctcggtcggg aacgacgcca ccatgtcggc gacgaggtcg tcgcgttgcg
      781 cacgatacgc ggggtcgtcc cacaggttgt gccattggtg gggatcctcg acgaggttgt
      841 agagctcgcc ctcggtgccg tcgtagtgga ccccgcacgg gcgcagcaca ctgtcgcccc
      901 agaccttctc gagaccgttc ggctgaccgt cggtgctctc ctcgtacgcc gtgcacatcc
      961 agccgtcgcg cacgatcgag cggaggtgca tgccgtagcc ggggaactgg ctgtcccact
     1021 ccgtcagcgc ccgttcgtgg acggccgtct gcccgaagct gtcggtcgtg gcctcgccgt
     1081 cgaacgtggg caggcgcgag ccctgcatcc acggcgcggg ttcgacgccg gcgatcgcgc
     1141 agaacgtggg cgccaggtcg acctggccga cggggtcggt gacgacggcg gcgtcgatgc
     1201 ccgccgacgc ggccggcttc cagatcagtg gcagtcgcat gagcgagtcg acgtgccagg
     1261 ggcccttgaa gatgaacccc atgtcgccct gcatctcacc gtgatcggtg gtgaacacga
     1321 tgtcggtgtc ggcgaggcgg ccgatggcgt cgaggtgcgc caggacgcgc ccgagcgcct
     1381 cgtcgagcag ctcgaccatg acgtgggtgc gggcgttgat ctcgcgcacc tggtcgtcgg
     1441 tgagggaggc ggggaagaag tacatggggc cgccctcggc attggcgaag gtgccgtcgt
     1501 accagcccag ccagtgcgcg ggcttgccgc cgagcacttc gcggatcttc tcggggctgc
     1561 cggggtgacc gggcgggagg tcgaggtcgc gccagttgac gcggcccatc tccgactcgg
     1621 gtgggtccca cgggtggtgg gggtcgggga agctcatcca gcagaagaac gggtcgtcgg
     1681 ccggcacggt gtcgagccag tcgatggtgc ggtcagccgt ccagtcggtg tggtaccagt
     1741 cggtcgggat ggggttgtgc ttggtctcgg gggccccggt gtcgccgccg ggcgcggcgt
     1801 tgagcagctc acagaacgag tcgaggtgct cggggtgctc ggccttgagc caccggccgt
     1861 agtgctggac gggtcgaccc tggaacgagg ggacgtgcgc cgcctggatc gagtagtcga
     1921 acccccgcca cgggccggtg tcattgcgcc gccaccggag gttctcctcc cagcggccct
     1981 ccatgtcgaa cgccggctcg aagtgggcct tgccgacgag ggccgtgtga tagccggccg
     2041 tcttgaggtg agcggcgatg ctcggcgcgt cggtcgggag tgcgatgccg ttggcgacca
     2101 caccgtgagt gcgggggtac tgcccggtga gcatcgtgca ccgcgccggc atgcagacgg
     2161 tgttgttgtt gtacgcgcgc tggtagttga tgccgtcacg cgccaaggcg tcggcgaccg
     2221 gtgtgcgggc gatcgtgcca ccgttgcacc cgagcgagtc gaagcgctgc tggtcggtcg
     2281 tcacgaagag aatgttgcga gccacatgtc ctcccttgcc ggtgagtcga tcacctcgga
     2341 gcgcgactgt caatatcttt tgacagtctg actcccagtt cttacgatcc ggtcatgggg
     2401 ctgcccgacc acgtcctcga cgactcggcg ctcgacgcca ccgtcatcga cggtcgccac
     2461 cagcggtcgg cgcgcagtcg cgacgccgtg gtcgaggcgc tgctctccct ctacgaggac
     2521 gggcacctgc gtcccggcgt ggccctcatc gcggaacggg ccggggtgtc gcagagctcg
     2581 gtattccgcc acttccaaga cgtcgacgag ttggtccgca gcgcgatcga ccatcagtgg
     2641 gagcgacttc gcgagctctt cacgcggccg ctcgtcggtg gatcactcga tgaacgcatc
     2701 gccgcgctcg ttgcgcagcg aatccgtctc tacgacatcg ccggccccgc gctccgggcg
     2761 gctcgcctgc tcgctccgga cagcgcggcc gtgcggtcgg ccttcgccct ccgacgtcaa
     2821 ctcctgcgcg gacaggtcga ggacctgttc gccgccgagc tggcggcgct cccctcctcc
     2881 cggcggcgaa cgacgctcga cgcgctcgac gtcgtcaccg gcttcgaagc ggtcgaggcg
     2941 ctgcgcatcg accgcggcgc cggtatcagg cgcactaccg acgtactcac actcaccatc
     3001 aaggggttgt tgtcatgacc gaagctccat ctcccgacga ccgccgcgtc gtcgtcctca
     3061 acgacgcccg gcgctacgtc ggaccggccc tgtcccgcct gctcgcggcg aggggccacg
     3121 acctcgtggt cggcgagatc gaagattccc ttgccgagga gctggccgcc ctcggcgcgt
     3181 cggtcgagat cgtggccggt ggtcgcgacc tgaccgaccc cgaggccgcc ggtcggctcg
     3241 ttgctgctgc acacgagcgg ttcggccgca tcgacgcggc ggtgtcgttc ggtggggtca
     3301 tcgtcaccgg ctcgttccgg cgttcgaccc ctgaggatct cgcccatgcc gtgagcggct
     3361 gcctcgaggc gccgtaccac ttcctcaagg ccgtcgtgga cccgatggtc gagcagggcg
     3421 tcgggcaggt gctcgtgatc acgagcgcag cgggcgctcg accgaccgac ggggcgccgc
     3481 tctactcgtc ggcccgcgcc gcggccaaca tgctggtccg caacgtcgct gccgaggtcg
     3541 cgggcaaggg cgtgcaggtc aacgccgtcg gaaccaactt catggacttc cccgacttcc
     3601 tccgggccac cggcgccgac gatccgtcgg tgcgcgctgc ggtcgagtcg atggtgccga
     3661 tgcatcgcct cggcaccatg gaggagttcg cctcgatgtg tctgccgttc atcgacggca
     3721 cgagccggtt cgtcaccggt cagttcattg cgtgcgccgg cggctgggcc tgagcccaac
     3781 cgccggccgt acggccctac agaccgaggt tgcacccgat ctcgaggaag ccggccgaga
     3841 tcggcacgac gatggcgccc tggcccggct ccgcgatgaa cagcgtgcag cccgtcacga
     3901 gcgggatgtt gagcggattg cccaccggaa tggcgcaggc gctcgacgag aacgccagca
     3961 cggcagcgag cgcgccgagg cggaacttct tcttggtgga acgagtcact ggataacccc
     4021 ctggttgatg gtcgcgccga gaggtcgacg ctgttctccc atgcagtgcc ccaggcactg
     4081 cgccggtcac cctaagggag ttctcagccc ggcccgatga cctccggtca attacccagg
     4141 cctccgaggc ggcgtcgagt gaaccgtgtc acattcctgc tgacagggcg cctccttgac
     4201 cgatcggtca actggggccg gtacgctcgc cgcgatgacc gtgatcacct tcgacgtcac
     4261 cgacgatctc tccggcgagg gtgggctccg aatcgaggcc gacgcctacg gcgacgcgct
     4321 ggctcgaccg gtcctcctgc tccacggtgg tggccagacc cggcactcct ggggttcgac
     4381 ggcggcgacc ctggcggcag ccggctggta cgccgtcgcg tacgaccagc gtggccacgg
     4441 cgcgagtggg cgctccccct cgggccacta cgagcccggg cgcttcgcgg aggatctcgt
     4501 ggagatcgcc acgacgttcg agcagccacc cgtcgtcgtg ggtgcgtcgc tcggtgggct
     4561 cgccgcgctc cttgccgagg gggtcctcgc cccgggcctg ttcgccggga tcgtgctggt
     4621 cgacatcacg ccccgtcagg aacgtgaggg cgtcaaccgc atcgtgtcct tcatgctcga
     4681 ccgcgccgtc gagggcttcg gttccctcga cgaggcggcc gacgccgtcg ctgcgtacca
     4741 gccccaccgc agccggcccc gggaccactc cggtctccag aagaaccttc ggctcgacgc
     4801 cgacggccgc tggcgctggc actgggaccc ggagctgttc aactccgaca acgggctcca
     4861 cgctgcccag gagcccggcc gtttcgtcgc cgcagctgca cagctcacgc tgccgacgct
     4921 gctcgtgagg ggcaagctga gcgatctggt gagcgaggag acagcgcagg agttcctcga
     4981 gctcgtcccc cacgcgcagt tcgtcgacgt gtccgacgcc ggtcacatgg tggcgggcga
     5041 ccgcaacgac cggttctgcg acgcggtcgt cgagttcctc gaaacgcttc cgacgaactg
     5101 accccgcgcc gtcgacgggc ggcgtgtcgc tcagacgcga cgggtggtgc ggaaccggat
     5161 cgcagcggtc cagaccaatg cgccggcagc cgcgaccggc acgagcgcca ggaagtcgcc
     5221 gaggtggatg ccgtgatcgt cgccgaggtc gacgagcacc ggaccggccc attggtgatg
     5281 accaaggagc agccagaacg ccgcgagtcc gagcgacacc gcggcgaggc cgaggagacc
     5341 gaggatctcc cggcgacgac cgacgagcgt gaccacgagc gcgaccaggg ccagcgtggc
     5401 catcgccgac ggtgccgaca gccagcgggt cggggtcaag cggacgtgcg cgtggaactg
     5461 gccgcggagc cccggcgagg tgacgagcac gacgccggcg gcgacgacca gcgcgtccca
     5521 gacgccgacg ccgaggcggc ggggccgctc ggcgagggtc gttggggcag gtggcgacgt
     5581 ggtcacgccc tcgacggtac cgagtccggc acgagtggat ccaccgccgg ctgggggcgg
     5641 tctcagccgg cggtcggtgt cagggcccgc cggtagtgtg cgggaccgtg accgatcagc
     5701 ctcctcccgc gcccgagcac gtcgacgatc cgtgggacgg tgtcgacatc gacaacgccg
     5761 tcgcatcgtt cttccgggcg ttggccgacg gtccgttgcg cgacgacgcg ctcctcgccg
     5821 cactccgtga cgacggtgcg tggggtgcca tcggtgagct cggagacgac gacttcgcgg
     5881 cggagatctc cgacctcgac gtgcccgggc tctggcgcac gcagatggcg atgtggttcg
     5941 acgtgttccg caccctcgac ggtcgagtgt tcacccatcg ggtcaccgcc gacgagttgg
     6001 ccgagggtgc ggtcgcagcg cttcccgacc tcacgctcgt cgactgggac gtcccggctc
     6061 gtcggatcgt cggcggttcg ggtgccatag ccaccgactt cgatcctgcc ttcgaccgtt
     6121 tcgagggcgg gcgcctgatc ggtcccgacg ggtggctcga cgggttcgct cccggcgacg
     6181 tactcggtct ccgcctcgtc cgtgacgaca ccgtgcgggt cgagcgcatc gacgccgccg
     6241 acctccccgg ccccgacgat tcggccgtgg cggagctggc gacggcgttc cgtccctgtt
     6301 tcgaccgctg gagcaacggc catggcgacg agctcgctcc cgccgtgctc gacctgttga
     6361 ccgacgcccc cgacgccttc cggaccgcaa cgctccccct cggcgagctg gccatcgccg
     6421 ccggcctcga acgccgagga ctcgagttcg gtcctgccga ccgcccgtgg ctgacccacg
     6481 cccagcaggc gttccgtcgc cgtggtgacg agctggccgc cgacctcgtc gattggctgg
     6541 cctcgccact gcgcacggtc gtcggcgtga tggtcgaatg gctcggtgac gccgacgcgc
     6601 tccccgcgtc gtcggtcgcc aaggcccgcg atgcgctggc caccgaaggg gttgccggca
     6661 tggccgggga gctcttccgg ggccacacag ctcccgggcg actcgctcgg ttcgtcgagg
     6721 cactcggcgg cccgggcaac gacgccgcca cgccctcgat tcacgtgctg gtcggcatcg
     6781 ccgccgagct cggcgggcgg ttcgacgatg ccgaacagga gttccgcgcc gccctcgacg
     6841 tcgacccggg ccagttggac gccgtggtcg agctgggcat cctcgcgtcg gaccgcggcg
     6901 acgccgttga ggccgagcga cgattccggt cagcggccgg gcccgaggcc tggctccggg
     6961 cgctgtcggg gttgctcccg tcgcgggcga cgggccgcaa cgaaccctgc ccctgcgggt
     7021 cgggtcgcaa gttcaaacag tgctgcctgg tgaatccgac gacgcccgtc gagcgccggg
     7081 gcccgtggta cgaactccgg ctcgcgcgtc atctcgccaa tcgcgacgcc ctggcgttcg
     7141 agatcttcta cggagtcctc tcggagtacc tgcccgagga ggccgagatc aacgatgcgc
     7201 tcgtcgacga cctggtctcg ctcgccaccg gcaacgtcgg gtcatatctc gaggcgcacg
     7261 ccgatcggct gagcgacgtc gatcgggccg gtctcgaggc ctggcgagac gcgccggtca
     7321 ccctccacca cgatcccgaa tccggtgtca cggcacgtcg cgtcgtcccg ttgggcacgg
     7381 ccgagtacgt ggtgtggtcg ttgcccctcg atgcgaccgc cgccgaggag ctcgaggggc
     7441 tgctcggcga cgacgacgct gtgacctccg tcgtggagtg ggccctctgg tacgcgtcga
     7501 ccttcgagga ggattgacgg gccgacgtcg cgcccaccgc gccccgagcg ccgccgaccg
     7561 cccgatcccc tcccccgctc cgccgggatc gggcgggtgg gtccgtcggc tgtggccatt
     7621 cctgatggca caccgccgca acgtgatcgt cgcgttcggc tccgccatcg tcggccaggg
     7681 ggtcgctgcg ctcgtgcccg tcatccagcg ggtcgtgatc gacgacggca tcgtcggacg
     7741 gacccggccg gtgggaccat ggctcgcagc gttggcggcg accgcggtct tcgtcttcgg
     7801 ctgctcctac ctccggcggt gggtcggcgg ccgggtcagc ctcgacgtcc agtacgacct
     7861 ccgcgtcgcc atcttcgaac ggctgcaacg gctcgacttc gccggccacg acgaactgca
     7921 gaccggccag ctcgtgtcgc gctcgtcgtc ggacgtcggg ctcatccagg ggttgctcgc
     7981 gttcctgccg atcatcgtcg gcaacgtggt gctcctcgtc gtggcgctgg tcgtgatggt
     8041 ggtgctgtcg ccgctgctca cactggtgat ggcggtgatg ctgccgctcc tgttggtcgt
     8101 gacgcttcgc atgcgacagt ggatcttccc tgccacctgg gacgcgcaac agcgcgccgg
     8161 tgtggttgcc ggggtcgtcg acgaggccgt cagcggcgtg cgggtggtga aggggttcgg
     8221 gcaggagcag cgtgagatcg accgacttgc cggagcagcc gagtcgctgt tcgcgtcgag
     8281 gagccggctg atccgcctca acgcccggta caccccggcg ctgcaggcca tcccgagcct
     8341 cgcccaggtc gccgtcctgg cgctcggcgg gtacctggcg atccacggtc acatcacgat
     8401 cggcaccttc ttggcgttct cgtcgtatct ggtgcagctc gtcgccccgg tccggatgtt
     8461 ggccagtctg ttcgccatcg gccagcaggc ccgcgccgga gccgaacgac tgctcgacgt
     8521 gctcgacgcc accgccgcgg tcaccgaccg tcccggggcc gtcgacctgc ccccggtccg
     8581 tggcgcgatc gccttcgagg acgtcacgtt cggctacgac cggtccgagc cggtgctgtc
     8641 ggggttcgat ctgcacatcg agcccggtga ggtggtggcg gtggtcggtg tgagtgggtc
     8701 gggcaagtcg acggtggcgg cgttggtcgc ccgcttctac gacgtgggca ctggccgggt
     8761 gacgatcgac ggcgtcgacg tgcgagacgt cacgctggcg tcgctccgcg gccagatcgg
     8821 ggtcgtgttc gaggacgcgt tcctgttctc cgacagcgtc cgcgacaaca tcgcgtacgc
     8881 cgtgcccggc gccaccgagg agcagatcgt cgtcgccgcc cgcgccgccg gcgcgcacga
     8941 cttcatcacc gcgttgcccg acgggtacaa cacgctcgtc ggcgagcgcg ggctcacgtt
     9001 gtcgggtgga cagcgacaac gggtcgccct ggctcgggcg atcctcgccg acagccgcgt
     9061 gctcgtgctc gacgacgcca cgtcgtcggt cgacagcatc accgaggagt cgatccacga
     9121 caccctccgc acgattgccg ccgaccgcac caccatcctg atcgcccacc ggcgttcgac
     9181 cttgcagctg gccgaccggg tggtcgtggt cgaggacggt cgggtggtcg aggagggcac
     9241 cgacgccgaa ctgacggcgc ggagcgagcg ctaccgccga ctgctcgccg gacccgacgc
     9301 cggcgacgac ctcggtgagg acgatcccga ctctgcgagc agcgcggcgc ctaccgacgg
     9361 gcgcaacgcg tggccggacg agccggcggc ggtcgtcggg ctcgccgggg ctgccgccgc
     9421 ggccgcgaag ccggcgatcg gtggcccggg cggaggtggc atgaacgtgg cgttcaccgc
     9481 gacccccgac ctcctcgaac ggctcgaacg gctcccgacg gcgaccgacc ggaccggcat
     9541 ggacgttgcc gcggcggccg ctccccaacg tggacgattc cgcttcgtgg ggttcatccg
     9601 tccgtggcgc cgtcccctcg ctctcggatt cgccctcgtc gcgctcgaca gtgggctcac
     9661 gctgctcggt ccgtggttcg tgcgccgtgg gctcgaccag ggtgtcgacg ctcgtgatac
     9721 cggggcgttg tggtgggcgt cggcggcgtt cgccgtcacg gtcgcgctcg actgggtggt
     9781 cacccggtcc tcgacactcg tcaccggtcg gacggcggag cgggcgctgg cgtcgcttcg
     9841 cgtcaggatc ttcgcccacc tccagcgcat gtccctcgac tactacgacg ccgagatgga
     9901 cggccggatc atgacgcgca tgacgaccga cgtggaggcc ctggcgcagg tcatccagac
     9961 cggcgtcgtc agcgcggtcg tcgctgcggt cacgtgtctc ggcgtcgccg tgttcctcgt
    10021 cgtcctctcc ccgccgctcg cgctggccgc ggccgtcgtg cttccgttcc ttgcgctcgc
    10081 cacgtggtgg taccggcgcc ggtcgtcggt cgcctacggg cgtgctcgcg acgccatcgc
    10141 cgacgtcaac gcagatctcc aggagaacct gtcgggggtg cgggtggcgc aggcgtcggt
    10201 ccgggaggag cgcaacctcg tccgctaccg acgggtcaac cagcggtacc tcgagcatcg
    10261 gttgggagcg caacggctca tcgccctcta cttcccgctc gtgttgttgc tcggcgacct
    10321 ggccgcggtg tcggtgctgg cgaccggctc ggcgctggtg caccgcggcg cgatcacgac
    10381 cgccgtcgtt atcgcgttcc tcctctacct cgaccagttc ttctcgccga tccagcagct
    10441 ctcccaggtg ctcgacacct ggcagcaggc cacggcgtcc ctcgtgaaga tcgaggagct
    10501 catgtcgacg ccgacgggca cgccggagtc gacgcacccc gtggagatcg ggcggctcac
    10561 gggtgcgatc gccctcgacg acgtgcactt ccggtacccg accgcgacgg gcgacgaggc
    10621 ggttgccgga gtcagcgtcc ggctcgacgc cggctcgacg gttgccctcg tcggctccac
    10681 cggtgcgggc aagtcgacga tcctcaagct catcgcgcgg ttctacgacc cggtgtcggg
    10741 cgccgtacgc gtcgacggtc gtcggcccga cggctcgagc gcggccgggg tcgacctgcg
    10801 tgacgtcggg ctcagctcct tccgtcggca actcggcatc gtcccccagg aggcgttcct
    10861 gttcgcgacg accgtccgcg acaacatcgc ctacgggcgg cccgacgcga ccgacgccga
    10921 ggtcgaggcg gcggcgcggg cggtgggcgc gcacgcgttc gtggcgtcgc tcccccacgg
    10981 ctaccgggag ccgatcagcg aacggggccg gtcgctgtcg tcgggccagc ggcagctgtt
    11041 ggcgctggcg cgggcccggc tcgtcgatcc gcccatcctc ctcctcgacg aggcgacgtc
    11101 caacctcgat ctgcacagcg aggcccgggt gcagcgggcc atgcggtccg ccgccgcggg
    11161 ccgaacgacg gtgctcgtgg cccaccgtct ccagaccgct cggctcgccg atcgcatcct
    11221 cgtcatcgag ggcgggagga tcgtgcagga cggcacccac gacgacctcc tcgccacgcc
    11281 gggtcgctat cgcgacctgt ggtcgagcta cgacggcgcg gtcggctgat accgcgcgat
    11341 cggcagggtg aggtcgagtt cctcgagcga ggtcaacgtg gcgtcggagt gggagtagtc
    11401 gagcgtccga ctgatcgggt tcgggacggc gacgcacgtg aggccggcgg ccttggcggc
    11461 tcgcaccccg ttcggcgagt cctcgagggc gagggcgccg gtcggcgcga ctccaatggc
    11521 ggcgcacgcc cggaggtagg tgtcgggcgc cggcttgccg ggaacaccgt tctcagcgca
    11581 gctgatggtc gcgaagcggt ggagcaggcc gcgcccggcc aggtggcccc cgacccactc
    11641 gaacgacgag ctgctcgcca cggcgacggg aaggcccagt tcgtcggcct ggtcgagcac
    11701 cccggtgacg ccggggcgga ggagctcggc agcgatggca gtgtccttga ggacgcgccg
    11761 cgccgcctgc acctcctccg aatcgaggtc acggtccacg gccgcctgga ggcgggcgaa
    11821 ggggtcgagc cggcggggat catcgtcgtg gagcccgagc gacgacagcc actcgtcgag
    11881 cgggatcggt tcggcaccgt ggtgctcgaa cgtggttcgc caggcgtcgt agagcggtgt
    11941 ctcggtgtcg aggatcagcc cgtcgaagtc gaacaccacg gcggtccacg gcgaaacatc
    12001 ggcagagcgc atccgacgat gctcaccgat cgaagcggct cccgtcgacc cggtttggcg
    12061 ccgactcggt cagtcgtcct cgccgggggt ccagcgaccc cgcggcgggg acacggcgcc
    12121 ggtgacgagc acacccttgc cgtcgccgag gcactggctc gagaggctgc tcgtgagggc
    12181 ggtgccggcc gacaggttgt agtcgttgcc gtcgtacccg gtggcggggt tgaacgggaa
    12241 ctgggctgcg gcgctccatg tcggtgtgaa cagctggcgc ccgaggctca ccttcactgg
    12301 cgtgaacgtt ggcttcttgg tgtcgcccac gctcggcttc acgagcgtgg tg
//
LOCUS       DAOSOA010000063        12321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5130_length_12321_cov_6.377548, whole genome shotgun sequence.
ACCESSION   DAOSOA010000063 DAOSOA010000000
VERSION     DAOSOA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12321
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5130_length_12321_cov_6.377548"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2112)
                     /locus_tag="PKY13_05970"
     CDS             complement(<1..2112)
                     /locus_tag="PKY13_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79513.1"
                     /translation="MFGQKIAAALVSVLAVCLVLPIPASAAPPVDLDGDGVADTLDID
                     DDGDGIIDVNETVANFQWATYNPISGSTATGTIGSNPFTYTASTTLQTTPGMYSVGTF
                     PARYNVPNANPTIKNTIASSNTLTFTQPILNPMVVFSSIGNPSTPVPVQFDRPYELLW
                     STGVAQNTTTRITGTEGFAILRFPGEHTTFSFDYLANETYVNFAFGADPRAAVDTDSD
                     GIVDSRDLDSDDDGIPDNVEAQTTAAMLVPSGADTDQNGLDDRYETTPGAGAGITRVD
                     TDSDGTVDALDVDSDGDGTRDVAESGLGLTDANADGRADGPVGTNGLADAAEAADGYA
                     DVNGLAHDGTSFLLADTDGDTAADGSDAAPRGQDLDYRDNRVDCNASDPDLDCDLDGR
                     PNGTDPHPSAATATADSMSARVGVTTTHDVLANDDYLPGSNTTITRRSGGNAGTAGGT
                     VSFDATTGLVSYRPAANEGGSSVTVGYSVCHTAGTPVCANAVVTVNVAVASSDLAVVA
                     TDAGPLAPGAHGGTAVTVSNAGPSDATNVVVVYSPPAGVTADMANLSGNCIADTPVAG
                     NVTCAYGTMLAADSGSVLIPVIVGTGTAPSTVATGADVDVTSDSTDPVGSNSTDQPAD
                     VTVAAASADLAIVLDAVPTLAPGATGQIDLDVHNNGPSPSPSFTVTYTLPPGVALDTA
                     GANPNACTRSAVTVTCPVIGPL"
     gene            2250..>3106
                     /locus_tag="PKY13_05975"
     CDS             2250..>3106
                     /locus_tag="PKY13_05975"
                     /inference="COORDINATES: protein motif:HMM:NF038209.1"
                     /note="Built with help from friend_finder, bracket5, and
                     grep mycofactocin; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-associated electron transfer
                     flavoprotein alpha subunit"
                     /protein_id="HOT79514.1"
                     /translation="MSGRPLAIVPVRAGVLPAGAAETVAEALAIGDADVVLVGSQLGS
                     AAGDLAGVADVVRLAEVGDFAPARWAAALSTLVDTAGVVLLPASPDGRDLAARVAAES
                     GRPLVGNAVSVTGTSATVLGWGGAVQREVHPRGPFVTTLQPGGRGVERGPGDAVRTLA
                     LDLELDDQLRDAVVTEIEPPDAATMDLAESPRIVGGGAGMMIGATDADPVDRRFAQLT
                     AIGEAIGASMGATRVVTDAGWVSHDRQIGTTGVVVDPDLYLSFGISGAVQHTAGLGHP
                     EHIISVNTDP"
     assembly_gap    3107..3206
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3207..4400
                     /locus_tag="PKY13_05980"
     CDS             <3207..4400
                     /locus_tag="PKY13_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOT79515.1"
                     /translation="PFPGSKNMYGGVIYGRILDGIIPNWWDEAPIQRWVTRRATMMMT
                     PTQAMTIDYRTTTWGEAPYNGATAYRPDFDNWLAGHAVAAGAELICSTTATGLLREGS
                     RVVGVRTDRPDGDLTADVVIACDGVNSFLAKEAGLFSHIEPEHYTVGVKETISLGKDT
                     IDERFGLRGLEGADFEMIGCTEGIPGGGFLYTNLDTVAVGVVLQVTALGAQSKRPEEI
                     IAGVKAHPSIAPLLEGGEVKEYSAHLIPEGGFNMMPELSCDGMLVAGDAAAMCLAAGI
                     WLEGVNFAIGSGIAAGQAAVDALRRGDTSDAGLAGYRQRMETNFVLADHRKLRGVADL
                     IMSERVQSIYPQLACNVAERIFTVENPRPKPGFNAIVRSEIKAAGVKLRDLAKDGITG
                     LKGYG"
     gene            4397..4702
                     /locus_tag="PKY13_05985"
     CDS             4397..4702
                     /locus_tag="PKY13_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT79516.1"
                     /translation="MSAANGDYPEISFEHRMDLVEFRVHERAHITVDGDSCRGCTTKA
                     CITACPANLFVPVSDGGILFNYEQCFECGTCYMVCNTEGAITWTYPEGGHGVVFKAS"
     gene            4711..5271
                     /locus_tag="PKY13_05990"
     CDS             4711..5271
                     /locus_tag="PKY13_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79517.1"
                     /translation="MSTIAVCVKWVDLHPEIDPLTGVVTTDPRRSGFSDSDQAALEVG
                     LRLAERRGGSVVLISVAPAVANDALRQLAASGAESLLHIPADDDLPSSAIGATIAAAV
                     TRLGDVELVVAGDHSLDRGSGSVPSFAAHGLGWPQALGLTDVTDDGRGGVRRLDGGRR
                     EHVRWDGPIVISVEGSVARLRRASLR"
     gene            5344..5538
                     /locus_tag="PKY13_05995"
     CDS             5344..5538
                     /locus_tag="PKY13_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79518.1"
                     /translation="MGPTSPVRPRARAFPAPHGDRALSRIVELTGALVERTPPRRVEA
                     DPAAAAAEIVGQLRVWGYLD"
     gene            5619..6275
                     /gene="mftE"
                     /locus_tag="PKY13_06000"
     CDS             5619..6275
                     /gene="mftE"
                     /locus_tag="PKY13_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HOT79519.1"
                     /translation="MTWPEVGERRPTLVVPLGSTEQHGPHLPLDTDARIATEVAQRLA
                     ATRVDLVVAPTVAIGASGEHAGFPGTLSVGAAVFEEMVVEIGRSADAFAGVVWVNAHG
                     GNSVALRSADARLRAEGRRSMIARCSFPGGDAHAGRTETSILLAIAPEVVRIELAAPG
                     DTRSWAEMEADIVAGGVVAVSPSGVLGDPTGASAEEGARLVDDLVAGLAAEVARWADD
                     "
     gene            6268..7071
                     /locus_tag="PKY13_06005"
     CDS             6268..7071
                     /locus_tag="PKY13_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HOT79520.1"
                     /translation="MTDLPATDRPVALVTGAARGIGAATARRLAADGFSVVLVDICAD
                     ISPLPYPMATKADLDASVAACGQHAVGVVADVRRQDELDAAVRTAVDEFGGLDVAVAA
                     AGIMGGGDPAWNISEEAFLVNIDINLTGVWRTAKATIPAMLERPKPRRGRFVAIASAA
                     GMRGNPTIADYTAAKHGVVGLVRSLALELGRRGITANAIAPGSTETAILEASGRLYGL
                     ESIDEFAVHHTIGRNLRPDEIAAGISWLCMPESSGVTGIVLPIDGGMTV"
     gene            7076..8470
                     /gene="mftF"
                     /locus_tag="PKY13_06010"
     CDS             7076..8470
                     /gene="mftF"
                     /locus_tag="PKY13_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HOT79521.1"
                     /translation="MRYRLDPSVEFLHGGRALLGGDPTKLLRLSEAGGALVARWRREG
                     CDPAGPAERQLVDRLVDAAMAHPVPDLEDRSLPAVIVPVRDRPDALERCLAALVASGP
                     TRIIVVDDGSIDPSAHAVVAERHGAAVVRRAISGGPAAARMTGWRALEPRPMLVAFVD
                     SDIEVSPDCWTALVGHFSDHACGLVAPRVAHRTGPGLIDRFEAANSPLDLGPDPAPIR
                     AGTRVSYVPAAALVVRAEAFEEVSGFDEALDVGEDVDLVWRLAEAGWRCRYEPASVVT
                     HVGRPELGPMLRRRFDYGRSAAVLDARHPGALPPVRGSAWSAAVVTAVAAGRPVVAAG
                     LTGWTVRAMAQRLGEVPDAWRLSARLVLRGHVGLARQLARAVIRPWFPITLALGLVSR
                     RVRRLAAVAAVVHPLLTWRERRPALPLPAWVALGLADDVAYSAGVWAGCVAARRLGPL
                     RPEFSQRSPDGPMG"
     gene            8564..9022
                     /locus_tag="PKY13_06015"
     CDS             8564..9022
                     /locus_tag="PKY13_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOT79522.1"
                     /translation="MGKPAVPADLLRADLAIEYPFNRTTGPVIGAFYTGLTKQVVVGI
                     KGVDGRVICPPVEYDPTTGEPLTELVKVSDEGTITSWSWVPAPRAQQALDAPHALALI
                     TLDGADTAILHVVAASGPEAVSTGARVKAHWADERSGAVTDIAYFELAGS"
     gene            9027..9602
                     /locus_tag="PKY13_06020"
     CDS             9027..9602
                     /locus_tag="PKY13_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOT79523.1"
                     /translation="MTDTDIYGVIPEGLWSQPAIDASAIAELDGVEPIQRVRLPASIT
                     YDYIPGLAPTRFLRGLAQKKILGERSAVLGDVYVPPRGMSPAEGLPTTEQVELPHKGS
                     VASFCVVHIGFGTNAPPTPFCSALVLLDGASVSVYGPIQGIEHDKVRIGMRVEPVWVD
                     DDQLAPSFESIKWWRPIDEPDVPAESLKGHM"
     gene            9602..10678
                     /locus_tag="PKY13_06025"
     CDS             9602..10678
                     /locus_tag="PKY13_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOT79524.1"
                     /translation="MADFQKAPRDVAIVSVTQDPHSRAVVERNEVELIQPIIQRALEQ
                     IGVQKGEIDFTCSGSSDYLAGQAFSFVMTLDAVGAWPPIAESHVEMDGAWALYEAWVK
                     IQSGHADTALVYSYGKSSPGDLPLVLSRQLDPYYYQPLWPDAIAMAGLQARQMLDAGL
                     ITERRMAEIAYESREAARNNPNAQLQGSPSVDELLAADFVSDPLRRHDCAPISDGGVA
                     VVLAAGDRARNWVDRPAWITGLDHRIDSHNFGARSLTSAPSAKLAAEKAGVGNDKIDV
                     AELHAPFTHQHQLLVEALGLGDNVSINPSGGALAANPMMAAGLLRLGEVANRIMAGEV
                     DRGVATATSGHLLQQNLVAVLEGA"
     gene            10680..11849
                     /locus_tag="PKY13_06030"
     CDS             10680..11849
                     /locus_tag="PKY13_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOT79525.1"
                     /translation="MGNRVAVIGIGQTKHAAVRGDVSLAGLLREAVYRALADAQLTMD
                     DIDAIVIGKAPDFFEGIMMPEGFLAEAIGAVGKPLLRVHTAGSVGGSTAIVAASHVQA
                     GVGERVLTIAWEQQSCSEAMWALSFPIPFQQQLVAGAGGYFAPFIREYMRRSNAPDDI
                     GILVALKDRLNALKNPYAHLHEPDITYDTVKDSFMLWEPIRYSETCPSSDGAIAMVLA
                     GEAAAEASAAATGTPPAWILGTSMRSEPATANGRDQVSPDAGKDCAADVFRQAGITNP
                     RAEIDVVEMYVPFSWYEPMWLENLGFCEEGEGWKLTEQGVTQLDGDLPVNCSGGVLST
                     NPIGASGMLRFGEAAMQVRGTAGEHQIDGAKTALGQAYGGAAQFFATWVVSSEKP"
     gene            12064..>12321
                     /locus_tag="PKY13_06035"
     CDS             12064..>12321
                     /locus_tag="PKY13_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79526.1"
                     /translation="MLLLAAIAASTLVWGAFGTGADSDAQLAQRELVELSAIRLQAMR
                     VTELAHLDLFRVAGDDVVDPSRLQGVLDTWQVDMAEVARRVP"
BASE COUNT         1701 a         4252 c         4302 g         1966 t
ORIGIN      
        1 gagcggtccg atcaccgggc acgtgacggt gaccgccgaa cgcgtgcagg cgttggggtt
       61 ggcgccggca gtgtcgaggg cgacgcccgg cgggagcgtg taggtcacgg tgaacgacgg
      121 tgacggtgac ggcccgttgt tgtggacgtc caggtcgatc tggcccgtgg cgccgggagc
      181 gagggtcggc acggcgtcga gcacgattgc caggtcggca gaagcggcgg cgaccgtgac
      241 gtcggcgggt tggtcggtgc tgttcgatcc caccgggtcg gtcgagtcgg acgtcacgtc
      301 gacatcggcg ccggtcgcca cggtgctggg cgccgtgccg gttccgacga tcacggggat
      361 caggaccgac ccggagtcgg cggcgagcat cgtcccgtac gcgcaggtca cgttgcccgc
      421 gaccggcgtg tcggcgatgc agttgcccga caggttggcc atgtcggcgg tcacgccggc
      481 cggaggggag tagacgacga cgacgttggt ggcgtcggac ggtccggcgt tggacaccgt
      541 caccgcggtg ccgccgtgcg caccgggagc gagcgggccg gcgtcggtcg ccaccactgc
      601 gaggtcggac gacgccacgg cgacattcac ggtcaccacc gcgttcgcac acacgggcgt
      661 cccggcggtg tggcagaccg agtacccgac ggtcaccgac gaaccgccct cgttggcggc
      721 cggtcggtag gagacgagtc cggtcgtcgc atcgaacgac acggttccgc cagcagttcc
      781 ggcgttgccg ccggagcggc gggtgatcgt ggtgttcgac ccgggcaggt agtcgtcgtt
      841 ggccaggacg tcatgtgtcg tggtcacccc gacccgcgcc gacatcgagt cggccgtcgc
      901 cgtggcagcg gacgggtggg ggtcggtccc gttgggtcgg ccgtcgaggt cgcagtccag
      961 gtccgggtcc gatgcgttgc agtcgacgcg gttgtcccgg tagtcaagat cctgcccacg
     1021 gggggcggcg tcggagccgt cggcagccgt gtcgccgtcg gtgtcggcga ggaggaacga
     1081 ggtgccgtcg tgggcgagtc cgttgacgtc ggcgtagccg tcggcggctt cggccgcgtc
     1141 ggccagacca ttcgtgccga ccggaccgtc cgcccgtcca tccgcgttgg cgtcggtcag
     1201 gcccaggccc gactcggcga cgtcccgggt gccgtctccg tcggagtcga cgtcgagcgc
     1261 gtcgacggtc ccgtcggaat cggtgtcgac ccgggtgatc ccggcaccgg caccgggcgt
     1321 ggtctcgtag cggtcgtcca agccgttctg gtcggtgtcg gctcccgacg ggacgagcat
     1381 tgcggcggtc gtctgtgctt cgacgttgtc ggggatcccg tcgtcgtcgg agtcgaggtc
     1441 gcggctgtcg acgatgccgt cgctgtcggt gtcgacggcg gcgcgggggt cggcgccgaa
     1501 ggcgaagttc acgtaggtct cgttggcgag atagtcgaac gagaaggtcg tgtgctcacc
     1561 ggggaacctg aggatcgcga atccttcggt tccggtgatg cgagtggtgg tgttctgggc
     1621 gacgccggtc gaccagagca gctcgtaggg gcggtcgaac tggaccggca cgggcgtcga
     1681 cgggttgccg atcgacgaga acaccaccat cgggttcagg atgggctgcg tgaacgtgag
     1741 ggtgttcgac gacgcgatgg tgttcttgat cgtcgggttg gcgttgggga cgttgtaccg
     1801 agcggggaac gtgcccaccg agtacatccc cggtgtggtc tgcagcgtcg tgctcgccgt
     1861 gtaggtgaac gggttgctcc cgatcgtgcc cgtcgccgtg cttccgctga tcgggttgta
     1921 ggtcgcccac tggaagttcg cgaccgtctc gttcacgtcg atgatgccgt caccgtcgtc
     1981 gtcgatgtcg agtgtgtcgg ccaccccgtc gccgtcgagg tccaccggcg gcgctgcgct
     2041 cgccggaatg gggaggacga ggcacactgc cagaactgac acgagggccg ctgcgatctt
     2101 ctgtccgaac atttttcgct ctgggttcac tcgaaatcga tcggcggagg agcgaagtca
     2161 atgagcagaa cttcccggtc ttttcctccg gccacccgaa cggggcgacc actgacgggg
     2221 ccccgatccg gcgcgggtaa cctgcaccca tgtccggccg ccccctcgcc atcgtgcccg
     2281 tgcgcgccgg tgtgctcccc gccggcgctg ccgagaccgt cgccgaggcc ctcgcgatcg
     2341 gtgatgccga cgtcgtgttg gtcggttcgc agctgggcag cgcggccggc gacctcgccg
     2401 gtgtggcgga cgtggtgcgg ctggccgagg tgggcgattt cgcccccgcc cgctgggcag
     2461 cagccctgtc gacgctggtg gacacggcgg gcgtggtgct cctgccggcg tctcccgacg
     2521 ggcgcgacct cgctgcccgc gtcgccgccg agtccggtcg gccgctggtc ggcaatgcgg
     2581 tgagcgtcac cggcacgagc gcaacggtgc tcgggtgggg cggcgcagtg cagcgcgagg
     2641 tgcacccccg cggtccgttc gtcacgacac tccaaccggg agggcgcggg gtcgagcgtg
     2701 gtcccggcga cgccgtgcgc acgctggcgc tcgatctcga gctcgacgat cagctccgcg
     2761 acgcggtcgt caccgaaatc gagccgcccg atgccgccac gatggacctg gcagagtcgc
     2821 cgcgcatcgt cggcggtggc gccggcatga tgatcggcgc gaccgacgcc gatccggtcg
     2881 accgtcgatt cgcgcagctc acggccatcg gtgaagcgat cggggcatcc atgggtgcga
     2941 cccgcgtcgt gaccgacgcc ggctgggtct cccacgatcg gcagatcggc accaccgggg
     3001 tcgtcgtcga ccccgacctg tacctgtcgt tcggcatcag cggcgccgtt cagcacaccg
     3061 ccggtctcgg ccaccccgaa cacatcatct cggtcaacac cgacccnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnccgt tccccggctc caagaacatg tacggcgggg
     3241 tcatctacgg ccgcatcctc gacggcatca tccccaactg gtgggacgag gcgccgatcc
     3301 agcgatgggt cacccgtcgg gccaccatga tgatgacgcc cacccaggcg atgacgatcg
     3361 actaccgcac cacgacctgg ggtgaggcgc cctacaacgg tgcaacggcg taccgtcccg
     3421 acttcgacaa ctggctcgcc ggccacgccg tcgccgcggg cgccgagctc atctgctcga
     3481 ccacggcgac cggcctgctc cgcgagggct cccgcgtcgt cggcgtgcgt accgaccgtc
     3541 ccgacggcga cctgaccgcc gacgttgtca tcgcctgcga cggcgtcaac tcgttcctcg
     3601 ccaaggaagc cgggctcttc tcccacatcg agccggagca ctacaccgtc ggggtgaagg
     3661 agacgatctc gctcggcaag gacacgatcg acgagcgctt cggtctgcga gggctcgagg
     3721 gcgcggactt cgagatgatc gggtgcaccg agggcatccc cggcggaggg ttcctctaca
     3781 cgaacctcga cacggtggca gtcggcgtcg tgctgcaggt gacggcactg ggtgcacaga
     3841 gcaagcgccc cgaggagatc atcgccgggg tgaaggccca cccgtcgatc gctccgctgc
     3901 tcgagggtgg tgaggtcaag gagtacagcg cccacctgat ccccgagggc gggttcaaca
     3961 tgatgcccga gctgagttgc gacggcatgc tcgtggccgg tgatgccgca gccatgtgcc
     4021 tggctgccgg catctggctc gaaggtgtca acttcgcgat cggttcgggc atcgccgccg
     4081 gacaggcggc ggtcgacgca ttgcgccgcg gcgacacgtc cgacgccggt ctcgccggct
     4141 atcgccagcg gatggagacg aacttcgtgc tcgccgacca ccgcaagctc cgtggtgtcg
     4201 ccgacctgat catgagcgaa cgcgtccaga gcatctatcc gcagctggcg tgcaacgtcg
     4261 ccgagcggat cttcaccgtc gagaaccctc gaccgaagcc ggggttcaac gcgatcgtcc
     4321 ggtccgagat caaggcggcc ggtgtgaagt tgcgtgacct cgcgaaggac ggcatcacgg
     4381 gtctgaaggg gtacggatga gcgccgcgaa cggggactac ccggagatca gtttcgaaca
     4441 tcggatggac ctcgtggagt tccgtgtgca cgagcgggcc cacatcactg tcgacggtga
     4501 ctcgtgccgc gggtgcacca ccaaggcgtg catcacggcc tgcccggcca acctgttcgt
     4561 gcccgtgtcc gacggcggca tcctcttcaa ctacgagcag tgtttcgagt gcggcacctg
     4621 ttacatggtc tgcaacaccg agggcgccat cacctggacc taccccgagg gtgggcacgg
     4681 cgtcgtcttc aaggcatcct gatcgctgcc atgtcgacga tcgcagtgtg tgtgaagtgg
     4741 gtggacctgc accccgagat cgacccgctc accggagtgg tcaccaccga cccccggcgg
     4801 tcggggttct ccgacagcga ccaggccgcg ctcgaggtgg gtctccgtct cgccgaacgg
     4861 cgcggcggca gcgtcgtgct gatctcggtc gcgcccgccg tcgcgaacga cgcgttgcgt
     4921 cagctggcag cgagcggggc ggagtccctg ctgcacatcc cggccgacga cgaccttccc
     4981 tcatcggcca tcggtgcgac gatcgccgct gcggtgactc ggctcggcga cgtcgagctc
     5041 gtggtcgccg gcgaccacag cctcgaccgc ggctccggct cggtgccgtc gttcgccgcg
     5101 cacggcctcg gttggcccca agcgctggga ctcaccgatg tcacggacga cggtcgcggc
     5161 ggtgtccgtc gcctggacgg tggccgacgt gagcacgttc gttgggacgg cccgatcgtc
     5221 atctcggtgg agggctcggt cgcgcgtctc cgtcgtgcat cgttgcgcta ggtgctcgcg
     5281 gcgaagtcgc ttccgggccc ggaccacgac accgcgccgg tgacggacct cagccgtgta
     5341 gacgtggggc cgacctctcc ggttcggccc cgggcccgag cgtttcccgc gccccacggc
     5401 gaccgcgccc tgtcgcgcat cgtcgaactc accggcgccc tcgtcgagcg cacacctccc
     5461 cgccgcgtcg aggccgaccc ggccgctgcg gcggccgaga tcgtcggcca gctgcgcgtg
     5521 tggggctatc tcgactgaga tgacctccga ccgttttgcc ctgcaaaccg ttcggtttgc
     5581 cgattgaaat gcaggacaaa acgagggtgt tgggcgagat gacgtggccc gaggtggggg
     5641 agcggcggcc cacgctcgtg gtgccgctcg ggtcgaccga gcagcacggt ccgcacctgc
     5701 cgctcgacac cgatgcccgc atcgccaccg aggtggcgca acgtctcgcg gcgacgcgtg
     5761 tcgacctcgt cgtcgctccg accgtggcga tcggcgcgtc gggcgagcac gccggcttcc
     5821 cggggacact gtcggtcgga gcggcggtgt tcgaggagat ggtcgtcgag atcgggcggt
     5881 ccgccgacgc gttcgccggg gtcgtctggg tcaacgccca cggcggcaac agtgtggccc
     5941 tgcgaagcgc cgacgcccgt ctccgcgccg agggtcggcg gtcgatgatc gcccgctgct
     6001 ccttccccgg gggcgacgca cacgccggac gcaccgagac gtcgatcctc ctggcgattg
     6061 cgcccgaggt ggtccgcatc gagttggccg cgccgggcga tacccgatcg tgggccgaga
     6121 tggaggccga catcgtcgcc ggcggcgtgg ttgccgtcag cccgagcggg gtgctgggcg
     6181 acccgacggg tgcctcggcg gaggaaggag cgcggctggt cgacgatctg gtcgccggcc
     6241 tcgctgccga agtggcacgc tgggccgatg actgacctgc ccgctaccga ccgacccgtc
     6301 gccctcgtca ccggtgctgc ccgaggcatc ggggcggcta ccgcccgccg cctcgcagcc
     6361 gacggcttct ccgtcgtgct ggtcgacatc tgtgccgaca tctcgccgtt gccgtacccg
     6421 atggcgacca aggccgatct cgacgcgtcg gtcgccgcct gtggacagca cgcggtgggt
     6481 gtcgtcgccg acgttcgccg tcaggacgag ctcgacgccg cggtgcgcac cgcggtcgac
     6541 gagttcggcg ggctcgatgt ggcggtcgcc gccgcgggca tcatgggcgg tggcgatccg
     6601 gcctggaaca tctccgagga ggcgttcctc gtcaacatcg acatcaacct cacgggcgtc
     6661 tggcgcaccg ccaaggcgac gatcccggcc atgctcgagc gccccaagcc gcggcgtggc
     6721 cggttcgtcg ccatcgcctc ggccgccggc atgcgtggca atcccaccat cgccgactac
     6781 acggcggcga agcacggggt cgtcggtctg gttcgttcgc tcgctctgga gctcggtcgg
     6841 cgcggcatca cggccaacgc catcgccccc ggctcgaccg agaccgccat cctcgaggcg
     6901 tccggtcggc tgtacgggct cgagtcgatc gacgagttcg cggtgcacca cacgatcggc
     6961 cgcaatctcc ggcccgacga gatcgcggcc ggcatctcgt ggttgtgcat gcccgagagc
     7021 agtggcgtca ccggcatcgt gttgccgatc gacggcggca tgaccgtctg atgaggtgag
     7081 gtaccggctc gacccgtcgg tcgagttcct gcacggcggc cgggcgctgc tcggcggcga
     7141 cccgacgaag ctgttgcgac tgagtgaggc gggcggagcc ctggtggcgc gctggcgtcg
     7201 ggagggatgc gatcccgctg gcccggcgga gcgccagctc gtcgaccgcc tggtcgacgc
     7261 ggcgatggcc cacccggtcc cggacctcga ggatcggtcg cttccggccg tgatcgtgcc
     7321 ggtccgggat cggcccgatg cgctcgaacg ctgcctggct gcgctcgttg catcgggacc
     7381 gacgcggatc atcgtcgtcg acgacgggtc gatcgatccg tcagcgcacg cagtggtggc
     7441 cgaacgtcat ggcgctgcgg tcgtgcggcg ggcgatctcg ggaggccccg cggcagcacg
     7501 gatgaccggc tggcgggcgc tcgaaccccg tccgatgctc gtcgccttcg tcgactccga
     7561 tatcgaggtc tcccctgatt gctggactgc cctcgtggga cacttcagcg atcacgcgtg
     7621 cggcctggtc gctccgcggg tcgcccatcg caccggtccg ggtctgatcg accggttcga
     7681 ggcggcgaac tcgccgctcg acctcgggcc ggatccggcg ccgatccgcg ccggcacgcg
     7741 tgtgtcctac gttcccgcgg ccgcgctcgt cgtgcgagcc gaggcgttcg aggaggtgag
     7801 cggattcgac gaggcgctcg atgtcgggga ggacgtcgat ctcgtgtggc gtctcgccga
     7861 agcggggtgg cggtgccgct acgagccggc atcggtcgtg acccacgtcg gccgccccga
     7921 gctcgggccg atgctgcggc gccggttcga ctacggccgc tccgcagcgg tgctcgacgc
     7981 gcggcatccc ggagcgttgc cgccggtgcg tggctccgcg tggtctgctg cggtggtgac
     8041 ggcagtcgcc gctggtcgtc ctgtggtcgc tgccgggctc accgggtgga cggtgcgggc
     8101 catggctcag cggctcggcg aggtgccgga cgcatggaga ctctccgccc gcctggtgct
     8161 gcgcggccac gtcgggctcg ctcgtcagct cgcgcgggcg gtgatccgtc cgtggttccc
     8221 gatcacgctc gcgctgggcc tcgtgagtcg gcgagtccgt cgcctcgccg ccgtcgctgc
     8281 cgtggtccac ccactgttga cgtggcgcga gcggcggccg gcgctccccc tgcccgcctg
     8341 ggtggccctc ggcctcgccg acgacgtggc gtactcggcg ggggtctggg ccggttgtgt
     8401 cgcggcccgg cggctcggcc ccctccgccc ggagttctcg cagcggtcgc ccgacggccc
     8461 gatgggctga attgccaaaa ctagaatccg ttctaatatt ccgcccgaca tgttctcgtc
     8521 ggccgcaacc tgtgcggacc gcaatgatcg aaggggagat gctgtgggga aaccagcggt
     8581 gcctgccgac ctgctccggg cggatctggc catcgagtac ccgttcaacc ggacgaccgg
     8641 tccggtgatc ggcgcgttct acaccgggct gaccaagcag gtcgtcgtcg gcatcaaggg
     8701 cgtcgacggc cgagtcatct gccccccggt cgagtacgac ccgaccaccg gtgagccgct
     8761 caccgagttg gtgaaggtct ccgacgaggg cacgatcacc tcgtggtcgt gggtgccggc
     8821 gccccgcgcg cagcaggcgc tcgacgcccc ccacgccttg gccctcatca cgctcgacgg
     8881 cgccgacacc gcgatcctgc acgtcgtcgc cgcctccggg cccgaggccg tgagcaccgg
     8941 tgcccgcgtc aaggcccact gggccgacga gcgcagcggc gccgtgaccg acatcgccta
     9001 cttcgaactc gccgggagct gagcgcatga ccgacaccga catctacggg gtgatccccg
     9061 agggcctctg gtcccagccg gcgatcgacg cctcggccat cgccgaactc gatggtgtcg
     9121 agcccatcca gcgtgtccga ctccccgcgt cgatcaccta cgactacatc cccggcctgg
     9181 cgcccacccg cttcctccgc gggctcgctc agaagaagat cctcggcgag cgttcagccg
     9241 tgctcggcga tgtgtacgtt ccgccgcggg gcatgtcgcc cgccgagggc ctgcccacca
     9301 ccgagcaggt ggagcttccc cacaagggca gcgtcgcctc gttctgtgtg gtccacatcg
     9361 ggttcggcac caacgcaccg cccaccccgt tctgttcggc gctggtcctc ctcgacggtg
     9421 cctccgtgtc ggtctacggg ccgatccagg ggatcgagca cgacaaggtc cgtatcggca
     9481 tgcgcgtcga acccgtctgg gtcgacgacg accagctcgc tccgagcttc gaatcgatca
     9541 aatggtggcg gcccatcgac gagcccgacg tgcccgccga gagcctgaag ggacacatgt
     9601 gatggccgac ttccagaagg caccccgcga cgtcgccatc gtcagcgtca cccaggaccc
     9661 gcactcccgc gcggtcgtcg agcgcaacga ggtcgagctg atccagccga tcatccagcg
     9721 ggccctcgag cagatcggcg tccagaaggg cgagatcgac ttcacctgct ccgggagctc
     9781 cgactacctc gccggtcagg cgttcagctt cgtcatgacc ctcgatgccg tcggcgcctg
     9841 gccgccgatc gcggagagcc acgtcgagat ggacggcgcc tgggcgctgt acgaggcctg
     9901 ggtcaagatc cagtccggcc acgccgacac ggccctcgtg tacagctacg gcaagagctc
     9961 gcccggcgac ctgccgctcg tgctgagccg ccagctcgac ccgtactact accagccgtt
    10021 gtggcctgac gcgatcgcga tggccggcct ccaggcccgc cagatgctcg acgccggtct
    10081 catcaccgag cgccgcatgg ccgagatcgc ctacgagtcc cgcgaggccg cccgcaacaa
    10141 ccccaacgcc cagctgcagg gttccccctc cgtcgacgaa ctgttggccg ccgacttcgt
    10201 gtcggatccg ctgcgtcgtc acgactgcgc cccgatctcc gacggtggtg tcgccgtggt
    10261 gctcgctgcc ggcgaccgtg cccgtaactg ggtcgatcgt ccggcttgga tcaccggcct
    10321 cgatcaccgc atcgacagcc acaacttcgg cgctcggagc ctcacgtcgg cgccgtcggc
    10381 gaagctcgcg gccgagaagg cgggcgtcgg caacgacaag atcgacgtcg ccgaactgca
    10441 cgccccgttc acccaccagc accagctgct ggtcgaggcc ctcgggttgg gcgacaacgt
    10501 gtcgatcaac ccatcgggtg gtgcgctggc cgccaacccg atgatggccg ccggcctcct
    10561 ccgcctcggc gaggtggcca accgcatcat ggctggcgag gtcgaccgcg gagtggccac
    10621 ggccacgagt ggtcacctcc tgcagcagaa cctcgtcgcc gttctggaag gagcctgaca
    10681 tgggcaaccg ggtagccgtc atcggcatcg gtcagaccaa gcacgccgcc gttcgtggtg
    10741 acgtgtccct cgccggtctc ctccgtgagg ccgtctaccg tgcgctcgcc gacgcacaac
    10801 tcacgatgga cgacatcgac gccatcgtca tcggcaaggc ccccgacttc ttcgagggca
    10861 tcatgatgcc ggaggggttc ctcgccgagg ccatcggtgc cgtcggcaag ccgctgctcc
    10921 gggtgcacac cgccggttcg gtgggtggct ccacggcgat cgtcgcggcg agccacgtgc
    10981 aggcgggtgt cggcgagcgc gtgctcacga tcgcctggga gcagcagtcg tgctcggagg
    11041 ccatgtgggc gctctcgttc cccatcccct tccagcagca gctggtcgcg ggcgcaggcg
    11101 gctacttcgc cccgttcatc cgcgagtaca tgcggcgctc gaacgccccc gacgacatcg
    11161 gcatcctggt ggcgctgaag gatcgcctga acgccctcaa gaacccctac gcccacctgc
    11221 acgagcccga catcacctac gacacggtga aggactcgtt catgctctgg gagccgatcc
    11281 gctactccga gacgtgcccc agctccgacg gtgccatcgc catggtgctc gccggcgagg
    11341 cagcggcgga ggcgtccgcg gcagccacgg gcacgccgcc ggcgtggatc ctcggcactt
    11401 cgatgcgttc cgagccggcg accgccaacg gtcgtgacca ggtgtccccc gatgccggca
    11461 aggactgtgc tgccgacgtg ttccgtcagg ccggcatcac caacccccgc gccgagatcg
    11521 acgtcgtcga gatgtacgtg ccgttcagct ggtacgagcc gatgtggctc gagaacctcg
    11581 gcttctgcga ggagggtgag ggctggaagc tcaccgagca gggtgtgacc cagctcgacg
    11641 gcgacctccc ggtgaactgc tccggcggtg tgctctcgac caaccccatc ggtgcgtcgg
    11701 gcatgctccg cttcggcgag gctgcgatgc aggtccgcgg cactgccggt gagcaccaga
    11761 tcgacggcgc caagacggcc ctagggcagg cctacggcgg tgcggcgcag ttcttcgcca
    11821 cctgggtcgt gtcctccgag aagccgtagt ccgtcggggg gcgccgcaac cccccacgag
    11881 aatcctcaga ccgaggtccc catcgtccga tgggaaggtg atccaccttt ccatcggacg
    11941 aggaccttgg ccggacgtca tcgaaccaac gtcgcccctg cagggctgac cgaacgtcgc
    12001 cgcggtgatc ggcgcgggat cgtcgtgtcg acgggtcgtc ctcgacgggt ccggttgttc
    12061 gtggtgctgc tcctcgccgc gatagcggcc tccacactcg tgtggggtgc gttcggcacc
    12121 ggtgccgaca gcgacgccca gctggcgcag cgcgagctgg tcgaactgtc ggccattcgc
    12181 ctgcaggcga tgcgtgtcac cgagttggcg cacctcgacc tgttccgggt cgccggtgac
    12241 gacgtggtgg atccttcccg cctccagggg gtgctcgaca cgtggcaggt ggacatggcc
    12301 gaggtggctc ggcgcgtgcc g
//
LOCUS       DAOSOA010000064        12074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5246_length_12074_cov_7.164573, whole genome shotgun sequence.
ACCESSION   DAOSOA010000064 DAOSOA010000000
VERSION     DAOSOA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5246_length_12074_cov_7.164573"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="add"
                     /locus_tag="PKY13_06040"
     CDS             complement(<1..851)
                     /gene="add"
                     /locus_tag="PKY13_06040"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOT79527.1"
                     /translation="MRDLLALPKGHLHLHLEGGMRPSTLVDLAEAAGIGVPVVSGFGS
                     FTAFADMYVAACEVLRTPDDLARLVDEAVEDASLSGSVWFEPSFYPPHHRERLGPDDE
                     VLEIVLEALAAASERHGVACGLMLASDRTQPVSEAIVLAELAVRYADRGVVSFGLAND
                     EVLGAPEAFAPAYDIIRDAGLLSTPHAGELCGPESVRGALDALGADRLQHGVRAVEDP
                     ALLARLAEEQVCCDVCPSSNVLLSVSPDLAGHQLVAMLEAGVPCSLNADDPLLFGPGL
                     LEEYEVCR"
     gene            complement(860..1726)
                     /locus_tag="PKY13_06045"
     CDS             complement(860..1726)
                     /locus_tag="PKY13_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT79528.1"
                     /translation="MTIDATTVVAPSASTGVLGAFAKRFAVTLGRAVFSLVLVLALWQ
                     FLVWTWGEQLSFSTRGPADVWKYLFGENSTPEIRSAIFSDLWITLGHAALGLFAGTAV
                     AVLVAFAFNVIRPLEQGLMPVAMVLRSVPLVAMAPLISTIFGRGLVTVAVVGGIVTFF
                     PTLVNVSLGLQSAPQQSVDLIKAYGGSRWYTLLKVQFPFALPPLFAALRATAPLAITG
                     AMLAEFFLTGTGLGSSLNLARNQFKYNSMWSQAAIATLFSVLIYALAVGIEGAVLARF
                     APDRAKAAKVKA"
     gene            complement(1737..2594)
                     /locus_tag="PKY13_06050"
     CDS             complement(1737..2594)
                     /locus_tag="PKY13_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT79529.1"
                     /translation="MNAAINVPVKTGPPIKHDEIVRNATVGERFSAWLRGAIPGALGF
                     AGFLIVWEIVGRVVNSGRQIPMIPPPTSVVDAILTDGWSYYRPNIGTTLGYALDGWVW
                     GNLLAVAAAGLVLMVPQLEIVILQLGVTTYCLPLVAVAPLVFLVYEGPTPYRIITILF
                     VFFTTLVLTLAGLRSADPTSLDVVRAYGGGRWQGLRRVRLISALPSIFAGFKIAGPAA
                     VLGAVLAEFFQPADSGLGVGIIRSQQQLEISRTWGIAVVSTMIGAAAYLLTTFVARVA
                     TPWAPKLEV"
     gene            complement(2621..3475)
                     /locus_tag="PKY13_06055"
     CDS             complement(2621..3475)
                     /locus_tag="PKY13_06055"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT79530.1"
                     /translation="MAATEQIIDLADRRDAAAAVSTAEAGSGIRLRGLTKEFTVKRKK
                     VTALDNADFDAPEGAFVALLGPSGCGKSTILRILADLEQPTSGTAEVHGAAPEEIRKR
                     HQLGIAFQDAALLPWRSVVSNIKLPLQVSGVKVSDEAISDLVKLVGLEGFEQARPGQL
                     SGGMRQRVAIARSLVIEPKVLLLDEPFGALDEMTRQRLNLELLRIWTERATTTLLVTH
                     SIAEAVFLADVVAVMSARPGRIVARIDIDLPRPRTPDMMRSPEFHAYCDQFSDLLFSG
                     GVAPSGDD"
     gene            complement(3615..4700)
                     /locus_tag="PKY13_06060"
     CDS             complement(3615..4700)
                     /locus_tag="PKY13_06060"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT79531.1"
                     /translation="MPSKIDRRRFLRNSAFGGAVMLGGPAFLAACGDDEKSSDSGSSG
                     SSSSGLGTLDVQLNWVKNAEYAGTWIADSEGYFTDAGFDKVNFIAGGPGVSQEELVIS
                     GKGLIGLTAPDFTASAVLEGAELVILGALFQKNPFCFMSLASNPVPDPEALKGKKVGV
                     QDVNQPTWQAFLKANSLDESEMTVVPVQFDPQGLVTGEVDAWFSFVTNEPNALKAEGI
                     DVEVFLLADHGYPMVSQCYFAKKETVESKADVLEAYLTADIKGWRESLKDPAKGAALA
                     ANVYGKDLGLKVEAATEESKSQNELILNEDTKTNGIMTVSDEMVEETIKSLGFAGLDV
                     TADQIFDLSLIEKIYADNPDLKADPTA"
     gene            complement(4916..5893)
                     /gene="add"
                     /locus_tag="PKY13_06065"
     CDS             complement(4916..5893)
                     /gene="add"
                     /locus_tag="PKY13_06065"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOT79532.1"
                     /translation="MLINLHSHLEGRVRPATAAELAQRAGVPEPAGGWEQAIQLDGPA
                     DLTVYLEKVASTYPLFTDLDAVARICAEAVEDAAAEGLDFLELRFGPATHTAPGRSID
                     DVLAAACAGLTDAVARTGMPAGLVPAALRHHDPETNDAVARAAARHAGRGVVGFDLAG
                     DELRFPHLSPYVTAFGIARAAGLGITCHAAEAAPGPAAREAVERFGATRIGHGAHIAS
                     DPATLGWAADEGVVIEVCPTSNWYTGAIGAVVDHPAPVFAAAGIPLVLGDDNPMQTRS
                     SLPAERAVLTDQLGFTDIQLAALDRTSVAAMFADDSVRAVLRSRLGTAS"
     gene            complement(5912..>6658)
                     /locus_tag="PKY13_06070"
     CDS             complement(5912..>6658)
                     /locus_tag="PKY13_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOT79533.1"
                     /translation="AGLDTRQFHIGIAPGEVGTVALLPGDPFRVPLIAEFLSDVRDVA
                     HNREHRTMCGTYKGKLITATSTGMGCPSTAIAVEELGRVGVTSFIRVGSSAGLQPGIA
                     PGDLLISEGALRNDGTTAAYAHPGYPAVPDLAITLELERRARELSEERGADRNVYAGI
                     NASDDAFYAETPAWISELNGLGVLNVEMESSALYVVARLRGWRAAMICSCSSNLVDGA
                     SLYGDTHDQLRDGWMASIEVALDTAVALDL"
     assembly_gap    6659..6758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6759..7513)
                     /locus_tag="PKY13_06075"
     CDS             complement(<6759..7513)
                     /locus_tag="PKY13_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT79534.1"
                     /translation="MNNNLFIVIAASAIYYGTPLFLAALGEVITERSGVLNLGVEGMM
                     LLGAVAASGTASNVSGPGWWVLTVSILAAMLAAGLGALLHAFATITLRVNQTVSGLAL
                     TILAGGAGLSSYLANLWELEKAPVQFEKFDVLGLKDAPIVGPILFNQNALTYLSWVLA
                     VVASWYLFRTRPGLHLRAVGESPQSADAVGINVVRYRYVHTVAGGALAGIGGAFVSLA
                     INTTWADGMTSGQGWIAIALVIFGFWRPDLTLAG"
     gene            complement(7510..8610)
                     /locus_tag="PKY13_06080"
     CDS             complement(7510..8610)
                     /locus_tag="PKY13_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT79535.1"
                     /translation="MIRLERRVDQPKWLAFAVPVGSLIAALLAGSLLIAFSGNDIWGT
                     YQRIVERAFTDDGALSSSILAATPLLFTGLAAAAAFRMGVFNIGGEGQFVMGAIAASG
                     VGLATGESLGAGAIPLMILAGVAAGAAWAAVVGALKAFFNTNEIIVSLMLNYIASNVA
                     YYLIFNSESYWRLLTPSGRQFPQGRPIDPSAEWTFTHIGPIDFPFGFWVGVVVAAITF
                     VLYRNTRYGFEVKVISDAPAAAKYAGMRTRRKVIAVMALSGALAGLGGASDVGDIRHL
                     LDPKGLRQAGYGYTGIVIAALARLNPLGVVFVSVLMGGLNNAGRSLQGPNFPAGLVGT
                     LQGLILFFALGGEVLARYRIRIQRNSKVVATS"
     gene            complement(8607..>9824)
                     /locus_tag="PKY13_06085"
     CDS             complement(8607..>9824)
                     /locus_tag="PKY13_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT79536.1"
                     /translation="REIEGRVRELGERYGLTVDPTAKVWQLSVGEQQRVEILKALERD
                     AKVLVLDEPTAVLTPQEAETLFVTLRAMAAEGRSIVFISHKLDEVMAVSDRVTVLRDA
                     RQVGTIDVASSSKRDLARLMVGRDVVFNADDGSDRPVVDRERVVLDVSDLTADDDRGQ
                     AAVDGVSLQLYAGEIVALCGVAGNGQRELAEAICATRHRTGGTVRVDGVATVGRDPRE
                     MIRLGVSHVPEDRLHTGLAASVTIEENLVLKSYRRPPISVGPFVKRRAVRSTATSLIE
                     RFGVKAPSPSTSTRLLSGGNVQKVLLAREFSAAPKVLIAASPTRGLDVGAIETVRALL
                     LEAARSGVAVLLMTEDLDEAIALSDRILVMYEGRIAGEVPRGHADITEIGLLMGGGRP
                     DEGTGDTDDGSAP"
     assembly_gap    9825..9924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9925..10941)
                     /locus_tag="PKY13_06090"
     CDS             complement(<9925..10941)
                     /locus_tag="PKY13_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT79537.1"
                     /translation="MKRKALVAALLAGSLLASACGKSDDSSDSSSDSGSSDGALKAAW
                     IYVGPTNDGGWTTAHDEGRKAVERALGDKVETTYKENVPEGPEVAQTIDDLVKDGNKI
                     IFATSFGFGDAMKAAAEKYPDVHFEWATGDAATDNMGIYFGAGEESLYLSGMAAGAAT
                     ESGTIGFVAPFPIPEVIRHINAYALGVQAVKPDAVVKVVWVNSWFDPTKEKQAAESLI
                     AGGADVIANGGDSPAPGEAAKAAKVAWTGYDSDQSENFPDEWLTAAVYDWGPYYTKQV
                     QAAIDGTWKAGSYYGDLADGFTNIAPFGKRVTEESRTAIEAKKSEIIEGSFNEFQGPI
                     VDQSG"
     gene            complement(11131..11829)
                     /locus_tag="PKY13_06095"
     CDS             complement(11131..11829)
                     /locus_tag="PKY13_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT79538.1"
                     /translation="MGARVTDAGPADVHLEFLVEPFVAGTPGPHVEAAIAAVEHHGVD
                     VESGPFANVAAGSSGDVAAAVASMLLAAFDRGATRVSTVVDLAVPGAPERPAPRSGRS
                     EPPSDASVNEFLAALRPVVRALGATVERTKRLEAGRGDIPLRWRGEVVGVVRPTPLTE
                     ALDHLIAQVEADLDSPLVSLDRSDKQRAVALLHERGAFALRRSTDAVAEAMGVSRITI
                     YNYLNALETPPESG"
     gene            complement(11814..>12074)
                     /locus_tag="PKY13_06100"
     CDS             complement(11814..>12074)
                     /locus_tag="PKY13_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT79539.1"
                     /translation="GAACLGRDDIGTLGVGKRADFAVWVTDDIADIADPLVGLVFGPD
                     RRVDRLVVGGRSVVEGGALVGVDLAEAHRRLAQRASRLWEHA"
BASE COUNT         1946 a         4015 c         4157 g         1756 t
ORIGIN      
        1 cggcacacct cgtactcctc caacagcccc ggcccgaaca gcaacggatc atcggcgttg
       61 agtgagcacg gcacgccggc ctcgagcatc gcaacgagct ggtgcccggc gagatcgggc
      121 gacaccgaga gcagcacatt ggacgacggg cacacgtcgc agcagacctg ttcctccgcc
      181 aaccgagcga ggagagctgg gtcctccacg gcgcgtaccc cgtgctgcag tcggtcggcg
      241 cccagcgcgt cgagcgcgcc ccggaccgac tcgggaccgc acaactcgcc ggcgtgcggg
      301 gtggagagca ggccggcatc gcggatgatg tcgtaggcag gagcgaatgc ctcgggtgct
      361 cccagtactt cgtcgttcgc gagtccgaag ctcaccacgc cgcggtcggc gtaccgcacg
      421 gcgagttccg ccagcacgat cgcttccgac accggctgcg tgcgatccga cgccagcatg
      481 agaccacacg ccaccccgtg ccgctcggac gcggcggcga gggcctccaa cacgatctcc
      541 aacacttcgt cgtcggggcc gagacgctcg cggtgatgcg gcggatagaa cgagggctcg
      601 aaccacaccg aacccgacag cgatgcatcc tcgacggcct cgtcgacgag tcgggcgagg
      661 tcgtcggggg tacggagcac ctcgcacgct gcgacgtaca tgtcggcgaa cgccgtgaag
      721 ctgccgaacc cgctgacgac gggaaccccg atgccggcgg cctcggccag gtcgacgagc
      781 gtcgacggtc gcatgccgcc ctcgaggtga aggtggagat ggcccttcgg gagcgccagc
      841 agatcccgca tgcaggtagt caggccttga ccttggcggc cttggcccga tcgggagcga
      901 atcgtgccag tacagcacct tcgatgccga cggccagcgc gtagatcagg accgagaaga
      961 gcgtggcgat cgcagcctgg ctccacatcg agttgtactt gaactggttc cgggccaggt
     1021 tcagcgacga gccgaggccg gtgccggtca ggaagaactc ggcgagcatc gcaccggtga
     1081 tcgcgagggg agccgtggcc cgcagcgccg cgaagagggg cgggagcgcg aacgggaact
     1141 gcaccttcag caacgtgtac cagcgactgc cgccgtaggc cttgatcagg tcgacggatt
     1201 gttgcggtgc gctctgcagg cccagcgaca cgttgaccag cgtcgggaag aacgtgacga
     1261 tgccgccaac caccgccacc gtcaccaggc cccggccgaa gatcgtggag atcagcggcg
     1321 ccatggccac gagcgggacc gaacgcaaca ccatcgccac cggcatcagc ccctgctcga
     1381 ggggacggat cacgttgaat gcgaaggcca cgagcaccgc gaccgccgta cccgcgaaca
     1441 gaccgagagc ggcgtgaccg agggtgatcc acaggtccga gaagatcgcc gagcggatct
     1501 cgggcgtcga gttctcaccg aagaggtact tccacacgtc ggcgggaccg cgggtggaga
     1561 acgacagctg ctcgccccag gtccacacga ggaactgcca gagcgccagc accaacacca
     1621 gtgagaagac ggcacggccc aacgtcacgg cgaaccgctt ggcgaacgcg ccgagcacgc
     1681 cggtgctggc cgagggtgcg accaccgtgg ttgcatcgat cgtcacggca gccacgtcac
     1741 acctccaact tcggcgccca cggcgtcgcc acccgggcga cgaaggtcgt gagcaggtag
     1801 gcggcggcgc cgatcatcgt cgacacgacg gcgatgcccc atgtacggga gatctcgagt
     1861 tgctgttggc tccggatgat gcccaccccg aggccgctgt cggccggctg gaagaactcg
     1921 gcgagcaccg cgccgagcac ggcagcaggt ccggcgatct tgaagccggc gaagatgctc
     1981 ggcagcgcgg agatcaggcg cacccgccgc aacccctgcc agcgcccgcc gccataggcg
     2041 cgcacgacgt cgagactggt ggggtcggcg ctgcgcaaac cggcgagcgt gagcaccagg
     2101 gtcgtgaaga acacgaacag gatggtgatg atccggtagg gcgtcgggcc ctcgtacacg
     2161 aggaacacga gcggtgcgac ggccacgagc gggaggcagt aggtcgtcac accgagctgg
     2221 aggatcacga tctccagctg cggcaccatg agcacgagcc cggcagccgc gacggcgagc
     2281 aggttgcccc acacccatcc gtcgagcgcg tacccgaggg tcgtgccgat gttgggccgg
     2341 tagtacgacc agccgtcggt gaggatggcg tcgacgaccg acgtcggcgg cgggatcatc
     2401 gggatctgac ggccggagtt cacgacgcgg ccgacgatct cccacacgat gaggaacccg
     2461 gcgaacccga gggcgccggg gatcgcgccc cgcagccacg cggagaaccg ctccccaacc
     2521 gtcgcgttcc ggacgatctc atcgtgcttg atgggtggtc cggtcttgac cggcacgttg
     2581 atggcggcgt tcacggctgc tcctcgatca tcgggcgccg tcagtcgtcg ccgctcgggg
     2641 cgacgccacc ggagaacagc aggtcgctga actggtcgca gtaggcgtgg aactcggggc
     2701 tgcgcatcat gtcgggcgtg cggggccgag ggaggtcgat gtcgatccgg gcgacgatgc
     2761 ggcccgggcg ggcgctcatc accgcgacga cgtcggccag gaacacagcc tcggcgatgg
     2821 agtgcgtgac gagcagcgtg gtcgtggccc gctcggtcca gatgcggagc agttcgaggt
     2881 tgagccgctg ccgggtcatc tcgtcgagcg caccgaacgg ctcgtcgagc agcagcacct
     2941 tgggctcgat gaccagcgat cgggcgatgg cgacacgttg gcgcatgccg ccggacaact
     3001 ggccggggcg ggcctgctcg aagccctcga gcccgacgag cttcacgaga tcggagatgg
     3061 cctcgtcgga gaccttcact cccgagacct gcagggggag cttgatgttc gacacgaccg
     3121 accgccacgg cagcaacgct gcatcctgga aggcgatgcc gagttggtgc cgcttgcgga
     3181 tctcctcggg agccgcaccg tgcacctcgg cggttcccga ggtgggctgt tcgaggtcgg
     3241 cgaggatgcg caggatcgtg gacttgccgc agcccgacgg ccccagcaat gcgacgaacg
     3301 ccccttcggg ggcgtcgaag tcggcattgt cgagcgcggt gaccttcttg cgcttgaccg
     3361 tgaactcctt ggtgagtcca cgcagtcgga ttcccgaccc ggcctctgcg gtgctcacgg
     3421 cagcggccgc atcgcgtcgg tcggcgagat cgatgatctg ttcggtggcg gccatgtcgt
     3481 cgtctccata cggttcattc gcagcggtag gtcggtgggt atcggtcgga ccgtggccac
     3541 aacggccggt gccggacccc gtccctgagg ggacgggtcc ggcatcgagc gttcgagcgg
     3601 ctccggtgcg gccgtcaggc cgtcggatcc gccttcaggt cggggttgtc ggcgtagatc
     3661 ttctcgatca gggagagatc gaagatctgg tcggcggtca cgtccaggcc ggcgaagccc
     3721 agcgacttga tggtctcctc gaccatctcg tccgacaccg tcatgatgcc gttggtcttc
     3781 gtgtcctcgt tgaggatgag ctcgttctgg ctcttcgact cctcggtcgc cgcctcgacc
     3841 ttgaggccga gatcctttcc gtagacattg gcggcgaggg ctgcgccctt ggccggatcc
     3901 ttgagcgact cacgccagcc cttgatgtcg gcggtgagat aggcctcgag cacgtccgcc
     3961 ttgctctcca ccgtctcctt cttggcgaag tagcactggc tgaccatcgg gtagccgtgg
     4021 tcggcgagga ggaacacctc gacgtcgatc ccctcggcct tgagggcgtt gggctcgttg
     4081 gtgacgaagc tgaaccaggc gtcgacctca ccggtgacga ggccctgggg gtcgaactga
     4141 accggcacca cggtcatctc ggactcgtcg aggctgttcg ccttcaggaa cgcctgccac
     4201 gtcggctggt tcacgtcctg cacaccgacc ttcttgccct tgagggcctc ggggtcgggg
     4261 acggggttgc tcgccaggct catgaagcag aaggggttct tctggaacag ggcaccgagg
     4321 atgaccagtt cggcaccctc gagcacggcc gaggccgtga agtcgggagc ggtgaggccg
     4381 atgaggccct ttccactgat gaccaactcc tcttgggaca caccgggacc gccggcgatg
     4441 aagttgacct tgtcgaaccc ggcgtcggtg aagtacccct cgctgtcggc gatccacgtg
     4501 ccggcgtact cggcgttctt cacccagttg agctggacgt cgagggtgcc gaggccgctc
     4561 gaggaactgc cggacgagcc ggagtccgag ctcttctcgt catcgccgca cgcggcgagg
     4621 aaggccgggc cacccagcat gacggctccg ccaaatgcgc tgttgcggag gaagcgcctc
     4681 cggtcgatct tcgatggcaa gactctctcc ttcgccccgg gttcggtgag cacaaccgtc
     4741 gtgacggtcg cggggagggg ctgggatgag attcgcggtg ccgtgtgcca ggcccgttgc
     4801 cgaactgtga acggctcgtt agcgccgtgt ccggctggac tgatcgtcac tggttccggg
     4861 tacgggcagc gccgacctga agaactgcat cacgaaccga cacctactgg tcgattcatg
     4921 atgcagttcc cagccgggac cggagaaccg cacggaccga gtcgtcggcg aacatcgcgg
     4981 cgacgctggt gcggtcgagc gcggcgagct gtatgtcggt gaacccgagc tgatcggtga
     5041 gcacggcccg ttcggccggg agtgacgacc gggtctgcat ggggttgtcg tccccgagga
     5101 ccaacgggat gccggccgca gcgaacaccg gcgctgggtg atcgacgacg gctccgatgg
     5161 cgccggtgta ccagttcgag gtcgggcaca cctcgatcac gacgccctcg tcagccgccc
     5221 acccgagggt ggccgggtcg gacgcgatat gcgcgccgtg gccgatgcga gtggcgccga
     5281 aacgctcgac ggcctcccgc gccgccgggc cgggcgccgc ctcggcggcg tgacaggtga
     5341 tgccgagccc ggccgcacgg gcgatgccga acgccgtgac atagggcgag aggtggggga
     5401 accgcagctc gtcaccggcc aggtcgaagc ccaccacccc gcgtcccgcg tgccgtgccg
     5461 ccgcccgagc gacggcgtcg ttggtctcgg ggtcgtggtg acgtagggcc gccgggacga
     5521 ggcccgccgg catgccggta cgcgccacgg catcggtgag cccggcgcag gccgccgcca
     5581 gcacatcgtc gatcgaacgg ccgggcgcag tgtgcgtcgc cggaccgaac cgcagttcga
     5641 ggaagtcgag gccctcagcg gccgcatcct ccaccgcctc ggcgcagatg cgggcgacgg
     5701 cgtcgagatc ggtgaagagg gggtaggtcg acgccacctt ctccaggtac acggtgagat
     5761 cggcgggacc gtcgagttgg atcgcctgct cccagccgcc ggcgggctcg ggcaccccgg
     5821 cccgctgcgc cagttccgcc gcggtcgccg gccggacccg cccctcgaga tgggagtgga
     5881 ggttgatcag cacggtagtg gtcgaccggg cctacagatc gagcgcgacg gcggtgtcga
     5941 gcgccacctc gatgctcgcc atccagccgt cgcgcaactg gtcgtgggtg tcgccgtaga
     6001 ggctggcgcc gtcgaccagg ttcgacgagc aggagcagat catggcggcg cgccagcccc
     6061 gcagccgggc gaccacgtac agcgccgagc tctccatctc gacgttgagc actcccagcc
     6121 cattgagctc gctgatccag gccggcgtct cggcgtagaa ggcgtcgtca ctggcgttga
     6181 tgccggcgta cacgttgcgg tcggcgccgc gctcctcgct cagttcccgg gcccgccgct
     6241 ccagttcgag cgtgatggcg aggtcgggca ccgccgggta gcccgggtgg gcgtaggcag
     6301 cggtcgtgcc gtcgttccgc aacgcaccct cgctgatcag caggtcaccg ggagcgatac
     6361 caggctgcag accggccgag ctgccgaccc ggatgaacga cgtgacgccg acgcggccca
     6421 gttcctcgac cgcgatggca gtggagggac accccatgcc ggtcgaggtg gccgtgatga
     6481 gcttgccctt gtaggtgccg cacatcgtgc ggtgctcccg gttgtgggcg acgtcgcgaa
     6541 cgtcgctcag gaactcggcg atcaacggca cccggaacgg gtcgccgggc aacagcgcga
     6601 cggtacccac ctcccccggt gcgatcccga tgtggaactg acgggtatcg aggcctgcnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ggcgagcgtc aggtcggggc
     6781 gccagaaccc gaagatgacg agggcgatgg cgatccagcc ctggcccgac gtcatgccgt
     6841 cggcccacgt cgtgttgatc gccagcgaca cgaacgcgcc gccgatgccc gccaacgctc
     6901 ccccggccac cgtgtgcacg tagcggtagc gcacgacgtt gatgcccacg gcgtcggccg
     6961 actgcggcga ctccccgacg gcgcgcaggt gcaggccggg acgggtgcgg aacaggtacc
     7021 acgaggcgac gaccgccagc acccacgaca ggtaggtgag cgcgttctgg ttgaacagga
     7081 tcgggccgac gatgggcgcg tccttcagcc cgagcacgtc gaacttctcg aactgcaccg
     7141 gcgccttctc gagctcccac aggttcgcga ggtacgacga cagtccggca ccgccggcga
     7201 ggatcgtcag cgccagaccc gacaccgtct ggttgacgcg gagcgtgatg gtggcgaagg
     7261 cgtggagcag cgcgccgagc ccggcagcga gcatcgccgc caggatcgag acggtgagca
     7321 cccaccaacc cggcccgctc acgttcgatg cggttcccga cgccgccacg gcgccgagca
     7381 gcatcatgcc ctcgactccg aggttgagga cccccgaccg ttccgtgatc acctcgccga
     7441 gcgcggcgag gaacagcggt gtgccgtagt agatcgcgga ggcggcgatc acgatgaaca
     7501 ggttgttgtt cacgatgtcg ccacgacctt gctgttgcgc tggatccgga tgcgataccg
     7561 ggccagcacc tcaccgccga gggcgaagaa gaggatcagc ccctgcagcg tgccgacgag
     7621 accggccggg aagttcgggc cctggagcga ccgaccggcg ttgttgagcc cacccatcag
     7681 caccgacacg aacacgacgc cgagcgggtt caaccgggcg agagcggcga tcacgatgcc
     7741 ggtgtagccg tagcccgcct ggcgcaaccc cttcggatcg agcaggtgac gaatgtcgcc
     7801 gacgtcgctg gcaccgccca ggcccgccag ggctccggag agcgccatga cggcgatgac
     7861 cttgcgacgg gtccgcatgc cggcgtactt cgccgctgcc ggcgcgtcgc tgatgacctt
     7921 cacctcgaac ccgtaccggg tgttgcggta gaggacgaac gtgatggcgg ccacgacgac
     7981 cccgacccag aagccgaacg ggaagtcgat cggaccgatg tgggtgaacg tccactccgc
     8041 cgacgggtcg atcgggcgac cctgggggaa ctgccggccc gacggcgtga gcagccgcca
     8101 gtacgactcg gagttgaaga tcaggtagta ggcgacgttg ctcgcgatgt agttgagcat
     8161 cagcgagacg atgatctcgt tcgtgttgaa gaacgccttg agcgctccga ccaccgccgc
     8221 ccacgcagcg ccggcggcga cgccggcgag gatcatcagc gggatggcgc cggcccccag
     8281 gctctcgccg gtggcgaggc cgacgcctga cgctgcgatg gcgcccatca cgaactggcc
     8341 ctcgccaccg atgttgaaca cacccatgcg gaaggctgcg gctgcggcga gcccggtgaa
     8401 gaggagtggc gtcgcggcga ggatcgacga cgacaacgcg ccgtcgtcag tgaaggcccg
     8461 ctcgacgatg cgctggtagg tgccccagat gtcgttgccg ctgaaggcga tgaggagcga
     8521 accggccagc aacgcggcga tcagcgaccc cacggggacg gcgaacgcca gccacttcgg
     8581 ttggtcgacg cgacgttcga gccggatcac ggcgcgctcc cgtcgtcggt gtcgccggtt
     8641 ccttcgtcgg ggcggccgcc gcccatgagc aggccgatct cggtgatgtc ggcgtgaccc
     8701 cgggggacct cgccggcgat ccggccctcg tacatcacca agatgcggtc ggagagagcg
     8761 atcgcctcgt cgaggtcctc ggtcatcagc aacaccgcca ccccggatcg ggccgcctcg
     8821 agcaacagcg ctcgaaccgt ctcgatcgca ccgacgtcga ggcctcgcgt cggcgacgcg
     8881 gcgatgagca ccttcggtgc ggcgctgaac tcccgcgcca acagcacctt ctgcacgttg
     8941 ccgcccgaca ggagccgtgt cgacgtggac gggctcggag ccttcacgcc gaaccgctcg
     9001 atcagtgacg tcgccgtgga tcggaccgct cggcgcttca cgaacgggcc gaccgagatc
     9061 gggggccgcc ggtacgactt gaggaccagg ttctcctcga tcgtgaccga cgccgccaag
     9121 ccggtgtgca accgatcttc gggaacgtgg gagacgccga ggcggatcat ctcccgcgga
     9181 tcccgcccga ccgtcgcgac cccgtcgacc cgaaccgttc caccggtgcg gtggcgggtc
     9241 gcgcagatcg cctcggcgag ttcgcgctgc ccattgccgg cgacgccgca caacgccacg
     9301 atctcgccgg cgtacagctg cagtgacaca ccgtcgaccg ctgcctggcc gcggtcgtcg
     9361 tcggcggtga ggtcggagac gtcgagcacc acacgctcgc ggtcgacgac ggggcggtcg
     9421 gagccgtcgt cggcgttgaa caccacgtcg cgaccgacca tgaggcgggc gaggtcgcgc
     9481 ttggacgagg acgctacgtc gatcgtgccg acctggcggg cgtcgcgcag cacggtgact
     9541 cgatcggaga ccgccatgac ctcgtcgagc ttgtggctga tgaagacgat cgagcgcccc
     9601 tcggccgcca tcgcccgcag cgtcacgaag agggtctcgg cctcctgcgg cgtgaggacc
     9661 gccgtcggct cgtcgaggac gagcaccttg gcgtcgcgct cgagcgcctt gaggatctcg
     9721 acgcgctgct gctcgcccac cgacagctgc cacaccttgg cggtcgggtc gacggtcagg
     9781 ccgtaccgct cgccgagctc gcggacacga ccctcgatct cgcgnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnngccgga ctgatcgacg atcgggccct ggaactcgtt
     9961 gaaggagcct tcgatgatct cggacttctt cgcctcgatg gcggtacggg actcctcggt
    10021 cacgcgcttg ccgaacggag cgatgttggt gaacccgtcg gccaggtcgc cgtagtagga
    10081 gccggccttc caggtgccgt cgatcgcggc ctggacctgc ttggtgtagt agggacccca
    10141 gtcgtacacg gcggcggtca gccactcgtc ggggaagttc tccgactggt cggagtcgta
    10201 gccggtccat gcgaccttgg cagccttggc ggcctcaccc ggtgcggggc tgtcgccgcc
    10261 gttggcgatg acgtcggcgc cgccggcgat cagcgattcg gcggcctgct tctccttggt
    10321 cgggtcgaac cagctgttga cccacacgac cttgacgacc gcgtcgggct tcacggcctg
    10381 gacgccgagg gcgtaggcgt tgatgtggcg gatcacctcg gggatcggga acggggcgac
    10441 gaagccgatc gtgcccgact cggtcgccgc accggcggcc atgcccgaca ggtagagcga
    10501 ttcctcgccg gcgccgaagt agatgcccat gttgtcggtg gctgcgtcgc cggtcgccca
    10561 ctcgaagtgc acgtcggggt acttctcggc ggcggccttc atggcgtcac cgaagccgaa
    10621 cgacgtcgcg aagatgatct tgttgccgtc cttcacgaga tcgtcgatcg tctgggcgac
    10681 ctcgggcccc tcgggcacgt tctccttgta ggtggtctcg accttgtcgc cgagggccct
    10741 ctcgacggcc ttgcggccct cgtcgtgagc ggtggtccac ccaccgtcgt tggtcgggcc
    10801 gacgtagatc cacgccgcct tgagcgcgcc gtccgacgag cccgagtccg agctcgagtc
    10861 gctggagtca tccgatttcc cacacgccga cgcgagcaat gagcccgcga gcagcgcggc
    10921 aacgagtgcc ttccgcttca tgtgttccct ttcgtgatga ccattcgatg ccacccggca
    10981 tggtcatgtc gttcgacacg ttcggccggc cgaagccgac gttctgtgaa ccggctcagc
    11041 cggattcgcc gagtcttgcc ggtgaccgaa cattgcacaa acgttcgcga aacgctctgg
    11101 cgacagcgtt cgatgtcggg atttcggggc tcagcccgac tcgggcggtg tttccaacgc
    11161 gttcagatag ttgtagatgg tgatgcgact gacgcccatc gcctcggcca cggcgtcggt
    11221 ggagcggcgg agcgcgaacg ctccccgttc gtggaggagc gccacggcac gctgcttgtc
    11281 ggaccggtcg agcgagacga gcggcgagtc gagatcggcc tcgacctggg cgatgaggtg
    11341 atcgagcgcc tcggtcagcg gcgtgggccg caccacaccg accacctcac cgcgccaccg
    11401 cagcgggatg tcgccccggc cggcctccaa ccgcttcgtg cgttcgacgg tcgcgcccag
    11461 ggcgcgcacc acgggacgca gcgccgcgag gaactcgttg acggatgcgt ccgacggcgg
    11521 ttcggaacga ccggagcgag gcgccggtcg ctccggcgct ccggggacgg cgaggtcgac
    11581 gacagtcgac acccgcgtcg cgcctcggtc gaacgctgcg agcagcatcg aggccaccgc
    11641 ggcagcgacg tcgcccgacg aaccagcggc gacgttggcg aacggccccg actcgacgtc
    11701 gacgccgtgg tgctcgacgg cggcgatcgc cgcctcgacg tggggaccgg gcgtgccggc
    11761 gacgaacggt tcgacgagga actcgaggtg cacgtcggcc gggccggcgt cggtcacgcg
    11821 tgctcccaca gtcgcgacgc tcgctgcgcg aggcggcgat gcgcctcggc gaggtcgacg
    11881 ccgacgagcg ctcccccctc gaccaccgag cggccgccga cgacgagccg gtcgacccga
    11941 cggtccgggc cgaacacgag acccacgagc ggatcggcga tgtcggcgat gtcgtcggtc
    12001 acccacaccg cgaagtcggc gcgcttcccg accccgagcg tgccgatgtc gtcgcggccc
    12061 agacatgccg cgcc
//
LOCUS       DAOSOA010000065        11970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5294_length_11970_cov_7.752497, whole genome shotgun sequence.
ACCESSION   DAOSOA010000065 DAOSOA010000000
VERSION     DAOSOA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5294_length_11970_cov_7.752497"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2072
                     /locus_tag="PKY13_06105"
     CDS             <1..2072
                     /locus_tag="PKY13_06105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOT79540.1"
                     /translation="PRWARGLNESQATAVSHEGPSLLVVAGAGTGKTGTLAARVARLI
                     EDGVPPNRILLLTFTRRAAQEMLARAGRLTDGAATAQVWGGTFHSVANRLLRTYGRGV
                     GLDPSFTVMDQGDALEMFGIVRAGVVETAGPRFPKKETIAAVYDQVVGTQRPLGDVVR
                     AEFPWVVGHLEALPPIFEEYTRRKRARRVLDYADLLLFWRGMLASREIGDEVAARFDH
                     VLVDEFQDTDPVQADIVHMMGDRGVSVTVVGDDAQSIYGFRAATVANMWSFGDRRPGT
                     RQVTLEDNYRSVGPILDVANAVLAQSDQHFDKHLRPKRPGTRRPLLVTCHDELGQARI
                     VADAILELREEGFPLSDQSVLFRTGYHSDVLELELGRRDIPYQKWGGLKFLEAAHVKD
                     LVSLLRVLDNPWDELAWNRVLRTLPGIGPATVRSMMEAMGLGDLDGIDLAAPARTPLD
                     RFLKDELKVPSAAREELDTLREAFADCLGHDGAEPAVAVQVERLTIWCEPSFDRRYDR
                     ADVRAGDLQQLQISAEAYTSRSRFLTELTLEPPASTGDLADEPHKDDDALTLSTVHSA
                     KGMEWGAVHIIHAADGNFPADMSLSTSEGLEEERRLMYVALTRARDVLSVYVPLRYHH
                     KRAGVGDRHSYAPISRFLAPVRSLFDESSDGSGADGHDPDSVIDVTAKVSVADEVDTI
                     TSGLWTI"
     gene            2219..3310
                     /locus_tag="PKY13_06110"
     CDS             2219..3310
                     /locus_tag="PKY13_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT79541.1"
                     /translation="MKPHSQKFRTSRAAVALLLAAATLSACGPSDSKSDDSAESGSSG
                     APASTISLVGFAVPKAANDALQEAWTNTADGENVTFETSYGASGDQSRAVEAGKQADY
                     VHFSLEGDVTRLVKAGLVAEDWNAGPTKGIVSDSVVVLVVRKGNPKNIEGWDDVVKPG
                     IGIVTPNPGSSGSARWNILAGWAHATAGSTEGDSGSDEDGKEFITKFFENAVTLAGSG
                     REATTAFQGGTGDVLISYENEAIFARQSGEDFEYIIPDDTLLIENPAAVTKDANPKAA
                     KLLEFARSDDGQKIFASKGFRPVSDIEVGEVEGANDPKDPFPAPKKLWTVADFGGWSD
                     VNKKFFATQEKDGADGIITAIQNASGKGN"
     gene            3312..4157
                     /gene="cysT"
                     /locus_tag="PKY13_06115"
     CDS             3312..4157
                     /gene="cysT"
                     /locus_tag="PKY13_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOT79542.1"
                     /translation="MTVRGRRFGRRSPSSGSGSGERLRLGSGIGLGVATIWLSILVLL
                     PLAAVVIKATDNGWSGFWDALSNPSTAAALRLTVTQAFLVTIVNVVLGTIVAWVLVRD
                     DFPGKGIVDLVIDVPFALPTIVAGLVLLSLYGPDSPVNLQFANTRVGIFIALLFVTVP
                     FVVRAVQPVLEELDRDVEEAAASLGADRFTIFRRIVFPNLIPAIAAGTALAFARAVSE
                     YGSLVLISGNLPFKTEVAAVRILSLNENDNPSGAAAVATVLLLVAVVTLVSIDGLRRW
                     AVKRG"
     gene            4150..5019
                     /locus_tag="PKY13_06120"
     CDS             4150..5019
                     /locus_tag="PKY13_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HOT79543.1"
                     /translation="MASAVLTPSAKAASRAQRRAGRKGPVQYLLRFVAVGYIGVVILI
                     PLFVVAQHTFADGASKVWEALTEENMLHAFEVTAYAAGYAVVINTVFGVGVSLLLVRY
                     RFPGRRILSLLVDLPLSVSPIVAGLALVFVYGRNGWFGPALDSAGFQVIFAFPGIVLA
                     TVFVSLPLVVREIVPVLEEIGTEQEQAATILGAGPFQTFRRITLPSIKWAVAYGVVLA
                     LARSLGEFGAVKVVTAGGVAGSTQTVTSLVEQRYGEFDELSAYSASLVLAATSIVCLV
                     VITLIRPKSDKKK"
     gene            5037..6098
                     /locus_tag="PKY13_06125"
     CDS             5037..6098
                     /locus_tag="PKY13_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HOT79544.1"
                     /translation="MGISVRNINKNFGDFVALGDVSVDIHSGNLTALLGPSGGGKTTL
                     LRIIAGLEQPDSGVIEINGTDTTWLRPQERNVGFVFQHYAAFKHLSVYRNVAFGLEIR
                     KVPKAQIRTRVMELLELVHLDQFADRLPAQLSGGQRQRMALARALAVEPNVLLLDEPF
                     GALDAKVRKELRDWLRRLHDEVHVTTVFVTHDQEEALEVSNEIVVINNGRVEQIGSPD
                     DLYDHPANEFVMSFLGSTTRLDGHLLRPHDVDVSTTAATGSVSAKVDRVLRIGFEVRV
                     DAVRPVAGGGDEVTTVQLTRKEANRLDLREGDEVWLSPAPGATTVIASAPISTVDDDL
                     DDDVLDVDVEAEEAAAPVG"
     gene            6195..7226
                     /locus_tag="PKY13_06130"
     CDS             6195..7226
                     /locus_tag="PKY13_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79545.1"
                     /translation="MSDDFLSADDLRAWVEQGVHEPTAEERLAEWERQVRAEELRIRL
                     EAAEDEAWEDERDRRHRRRNRRIVVAGWLVVFALIAGLLVRTFALSDDRRAASAWAAL
                     DRADVGTRVGSGEMPSPPISVSPTPLGTPIDVPSKRGPYEFVSMQPGSDAPVTYDPCR
                     PIHYVVNHTGQPNDAENLVTDAIERISEVTGLQFVADDLTDEEIVPDRQPYQPDRYGD
                     RWAPVLIAWGTPDDTPLLAGSVAGFGGSTQYTVTDQAGISRSVYVTGEVVLDGPQIAN
                     IIRLDRDGLDGARAVVLHELAHVVVLDHVDDPTQLMNPVSQPGVTDFAAGDLRGLRQL
                     GLGACVPGI"
     gene            7287..7964
                     /locus_tag="PKY13_06135"
     CDS             7287..7964
                     /locus_tag="PKY13_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79546.1"
                     /translation="MHRVAGRIVVGLVVIAMSMVFGAGRAAACSCIGLEYLVQNEAEF
                     PIEAAFAGTVTAVPTIDYSVPVVTWSFRIDRVYRGDVGNVGDTIDVRSSASGASCGFE
                     YMTVGDRRDIALQQDGGEWTSGLCSQGAVGTLAPLAGASTTVPPPDTTSATPPTIETT
                     TSGPSETTTSSTPDEGSVTISSDEPTGNEGGSSGLAMGAGAIGATTLIAGAMIVVRRR
                     QNGVASD"
     gene            complement(7961..11752)
                     /gene="hrpA"
                     /locus_tag="PKY13_06140"
     CDS             complement(7961..11752)
                     /gene="hrpA"
                     /locus_tag="PKY13_06140"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOT79547.1"
                     /translation="MEPAITYPPDLPISERRDDLVAAIGAHQVLVVAGETGSGKSTQL
                     PKLCLEAGRGAKDATTGRGGWIGHTQPRRIAARSIAERVAEELGSEVGQAVGYKVRFN
                     DEVGTDTRIKVMTDGILLAEIQRDRELQKYDTIIIDEAHERSLNIDFLLGYLRQLLPR
                     RPDLKLIITSATIDTARFSEHFDGAPIIEVEGRTFPVEVRYRPLDDPDDPAGPKDQVE
                     GICDAVEELRREVDGDILVFCSGEREIRDAAAALGDLHLPGTEVFPLFARLSAAEQHR
                     VFERHAGRRIVLATNVAETSLTVPGIRSVIDPGTARISRYSYRTKVQRLPIEDISQAS
                     ANQRSGRCGRIGPGIAVRLYDEEDFEARPEFTEPEIRRTNLASVILQMANLGLGDVAS
                     FPFVDPPADRNIRDGIALLEELGAVDPDQYGTRKWLTTLGRRLVRMPVDPRLGRMVIE
                     AGTNGCLHEVMVITAGMSIQDPRERPFDHRTQADQQHVRFNHPDSDFLSYVNLWNYLG
                     EQRRERSSNQFRKMCRNEFLNYNRIREWQDIYEQLRRATKQLGFKPNTVPAKPDAVHL
                     SLLSGLLSHIGMKDTPEKGREPRGRDGGRDGREQRDRRGTREYIGARQARFTIAPGSV
                     LAKNPPNWVMAGELVETNRLWARTATRIDPAWVERLGEHLAKYTYSEPWWERERGAAM
                     THERVTIYGLTLVSGRRVPVARVDPAMARDLFIHHALVEGDWDTHHKFMDRNAVVAGE
                     AERLEARARRGDLIVENQAIFDFYDQRVPDQVTTGAAFNQWWKDERRANGKLLDLTLD
                     DLLADDAHAAVDPHAFPEVWHYGAHDLPITYEFDPTSEADGATITIDAGIVGQLEPAA
                     FDWNVPGFRDDLVDALIRSLPKQLRKLFVPVPETVAVIGERITPDNGPVLESVRRELN
                     RLITEPLPVDAFDLDALPKHLRPSFRIVDTDGRELAIGRDLRALAKQVHAEATAIVAT
                     VDTGLEQRGLRTWTFGDLPRTVERNVNGRVITAYPALADDGDSAAIVACTSSDEQIAT
                     MWAGTRRLVRLAMPAPARQVDSLLDQRTKNAMAQSPVQPKVDWYNDIIGCALDHLIAE
                     AGGPVWTGAAFERLVDAVRDRLHDTLVTVTASTAKLIALHSSIRRTLNQLVAAPMASA
                     VTDVREHLDRLVYEGVLTGVGFAKLDDIDRYLRAIERRLANLPNNPKRDADLMERCRK
                     LEAEYENLAETVSPSDDLEALAWALEEFRVSSFAQQLRTPIPVSEKRIRNEIHRLARG
                     L"
BASE COUNT         1776 a         3889 c         4269 g         2036 t
ORIGIN      
        1 tgccccggtg ggctcgcggg ctcaatgagt cccaggcgac cgcggtctcc cacgaggggc
       61 cgtcgctcct ggtggtggcg ggtgccggca cgggcaagac gggcacgctc gcggcgcggg
      121 tcgctcgcct catcgaggat ggtgtgccgc ccaaccggat cctgctgctc acgttcacac
      181 gccgtgcggc ccaggagatg ttggcccgtg ccggtcgcct caccgacggc gccgcgaccg
      241 cgcaggtctg gggcggcacg ttccactccg tcgccaaccg cctgctgcgc acctatggca
      301 ggggagtggg gctcgacccg agcttcaccg tgatggacca gggcgacgcg ctcgagatgt
      361 tcggcatcgt gcgcgccggt gtcgtcgaga cggccgggcc gcggttcccc aagaaggaga
      421 cgatcgctgc ggtgtacgac caggtggtcg gcacgcagcg gccgttgggc gacgtcgttc
      481 gtgccgagtt cccgtgggtg gtcggccatc tcgaggcgct cccaccgatc ttcgaggagt
      541 acacccgtcg caagcgtgct cggcgcgtgc tcgactacgc cgatctcctg ttgttctggc
      601 ggggcatgct ggcctcccgc gagatcggcg acgaggtcgc tgcccgcttc gaccacgtgt
      661 tggtcgacga gttccaggac accgatccgg tgcaggccga catcgtgcac atgatgggcg
      721 atcggggtgt gtcggtgacc gtcgtcggcg acgacgcaca gtccatctac ggcttccgtg
      781 ctgcgacggt cgccaacatg tggagcttcg gcgaccggcg gcccggcacg aggcaggtga
      841 ccctcgagga caactaccgg tcggtcgggc cgatcctcga cgtcgccaat gcggtgttgg
      901 cgcagtccga tcagcacttc gacaagcacc tgcgtccgaa acgtcccggc acccgccgcc
      961 cgctcctggt cacgtgccac gacgagctgg gtcaggcgcg aatcgtcgcc gacgcgatcc
     1021 tcgagctgcg cgaggagggc ttcccgctca gcgaccagtc ggtcctgttc cgcaccggtt
     1081 accactccga cgttctggag ctggagctcg gccgtcgcga catcccgtac cagaagtggg
     1141 gtggcctcaa gttcctggag gcggcgcacg tcaaggatct ggtgtcgctc ctgcgggtgc
     1201 tcgacaatcc gtgggacgag ctggcgtgga accgtgtgct gcgcacgttg cccggcatcg
     1261 gcccggcaac ggtgcgctcg atgatggagg cgatggggct cggcgacctg gacggtatcg
     1321 acctcgcagc gccggcccgc actccgctcg accggttcct caaggacgag ctgaaggtgc
     1381 cgagcgcggc ccgcgaggag ctcgacacgt tgcgcgaggc gttcgccgat tgtctcggtc
     1441 acgacggcgc ggagccggcg gtggcggtgc aggtggagcg gctcacgatc tggtgcgagc
     1501 cgagtttcga tcgccgctac gaccgggccg acgtgcgcgc cggcgacctg cagcagctcc
     1561 agatcagtgc cgaggcgtac acgtcacggt cgcgcttcct gaccgagctc acgctcgaac
     1621 cgccggcctc gaccggcgac ctggccgacg agccgcacaa ggacgacgac gccctcacac
     1681 tctcgaccgt gcattcggcc aagggcatgg agtggggtgc ggtgcacatc atccacgctg
     1741 cggacggcaa cttcccggcc gacatgagcc tgtcgacgag cgaggggctc gaggaggagc
     1801 ggcggctcat gtacgtcgcg ttgacccgcg cccgtgacgt gctgagcgtc tacgttccgc
     1861 tccgctacca ccacaaacgc gccggggtgg gcgaccgcca ttcgtacgcg ccgatcagcc
     1921 ggttcctggc gccggtgcgt tcgttgttcg acgagtcgtc cgacggcagt ggcgccgatg
     1981 gccatgatcc cgactcggtc atcgacgtga ccgcgaaggt gtccgttgcc gacgaggtcg
     2041 acaccatcac gtccggtctc tggaccatct gagacccgaa atgtgctgcg agcgtcgcgc
     2101 cgagcggcga cgctcgcgtc acgtttgcgt cacgtttgcg gccgggactc gatacctgac
     2161 tatttgagtc ggatttattc ttgaccaatt cggtcaagat tacctagggt cgtcggccat
     2221 gaagccccac agccagaagt tccgcacgtc ccgagcagcg gttgcgctgc tcctcgccgc
     2281 cgcaaccctg tccgcctgcg gaccgagcga cagcaagtcc gacgacagcg ccgagtccgg
     2341 ctcgtccggc gcacccgcgt ccaccatctc cctggtcggg ttcgccgtcc cgaaggcggc
     2401 gaacgacgcc cttcaggagg cgtggacgaa caccgccgat ggcgagaacg tcaccttcga
     2461 gacctcctac ggcgcgtcgg gggaccagag ccgcgccgtc gaggccggca agcaggccga
     2521 ctacgtgcac ttctcgctcg agggcgacgt cacccgtctc gtgaaagccg gcctggtcgc
     2581 cgaggactgg aacgccggac ccaccaaggg catcgtgtcc gactccgtcg ttgtcctcgt
     2641 cgtccgcaag ggcaacccca agaacatcga gggctgggac gacgtcgtga agccgggcat
     2701 cggcatcgtc acccccaacc ccggatcctc gggctcggcc cggtggaaca tcctcgccgg
     2761 ctgggcgcac gccacggcgg gttcgaccga aggcgacagc gggtccgacg aggacggcaa
     2821 ggagttcatc accaagttct tcgagaacgc cgtgacgctc gccggctcgg gtcgtgaggc
     2881 cacgaccgcg ttccagggtg gaaccggtga cgtactcatc tcctacgaga acgaggccat
     2941 cttcgcccgc cagagcggtg aggacttcga gtacatcatc cccgacgaca ccctcctcat
     3001 cgagaacccc gccgccgtca ccaaggacgc caaccccaag gctgcgaagc tcctcgagtt
     3061 cgcgcggtcc gacgacggcc agaagatctt cgccagcaag ggcttccgac cggtgagcga
     3121 catcgaggtc ggtgaggtcg agggagcgaa cgatcccaag gacccgttcc cggcgccgaa
     3181 gaagctctgg acggtcgccg acttcggcgg ctggagcgac gtcaacaaga agttcttcgc
     3241 cacccaggaa aaggacggtg ccgacggcat catcactgcc atccagaacg cttcaggcaa
     3301 gggcaactga ggtgacggtc cgtggtcggc ggtttggccg tcggtcaccg tcgtcggggt
     3361 cgggctccgg tgaacgactc cgcctcgggt cgggcatcgg gctcggtgtc gcgaccattt
     3421 ggctgagcat cctcgtgctg ctcccgctcg cggccgtcgt gatcaaggcg accgacaacg
     3481 gttggtcggg gttctgggac gcgctctcga acccttcgac ggccgcggcc ctgcggctca
     3541 cggtaacgca agcgttcctc gtgaccatcg tgaacgtggt gctcggcacc atcgtcgcct
     3601 gggtgctcgt tcgcgacgac ttccccggta agggcatcgt cgacctcgtc atcgacgtgc
     3661 cgttcgccct accgacgatc gttgcgggcc tcgtgctgct gtcgctctac ggccccgaca
     3721 gtccggtgaa tctccagttc gcgaacaccc gggtcgggat cttcatcgcc ctgctgttcg
     3781 tgacggtccc gttcgtggtg cgggcggtgc agccggtgct cgaggaactc gaccgcgatg
     3841 tcgaggaggc tgcggcgtcg ctcggagcgg accggttcac gatcttccgc cgcatcgtgt
     3901 tcccgaacct gatcccggcg atcgctgcgg ggaccgccct cgccttcgcc cgtgcggtga
     3961 gcgaatacgg ctcgctcgtc ctgatctcgg gaaacctccc gttcaagacg gaggtcgcgg
     4021 cggtgcgcat cctgtcgctc aatgagaacg acaacccgtc gggcgcagcg gcggtcgcca
     4081 cggtgttgct gctcgtcgcg gtcgtgaccc tggtgtcgat cgacggtctc cgtcgctggg
     4141 cggtgaagcg tggctagcgc agtgctcacc ccgtcggcga aggcggcgtc cagggcgcag
     4201 cggcgcgccg gccgcaaggg tccggtgcag tacctgctgc gcttcgtcgc ggtcgggtac
     4261 atcggcgtgg tcatcctcat accgctgttc gtggtggcgc agcacacgtt cgccgacggc
     4321 gcttccaagg tgtgggaggc gctgaccgag gagaacatgc tccacgcgtt cgaggtgacg
     4381 gcctacgccg ccggttacgc cgtggtcatc aacacggtgt tcggcgtggg cgtctcgctc
     4441 ctcttggtcc ggtaccgctt cccgggtcgg cggatcctga gcctgctggt cgacctcccc
     4501 ctctcggtct cgccgatcgt cgccggcctg gcgctcgtgt tcgtgtacgg ccgcaacggc
     4561 tggttcggtc cggcgctcga cagcgcgggg ttccaggtga tcttcgcctt ccccggcatc
     4621 gtcttggcca ccgtgttcgt gtcgttgccg ctggtcgtgc gtgagatcgt gccggtgctc
     4681 gaggagatcg gcaccgagca ggagcaggcc gccacgatcc tcggcgccgg gccgttccag
     4741 acgttccggc gcatcacgtt gccgtcgatc aaatgggcgg tcgcgtacgg cgtcgtgttg
     4801 gccctcgctc gcagcctcgg cgagttcggg gcagtgaagg tcgtgaccgc cggtggcgtg
     4861 gccggcagca cgcagaccgt cacgtccctc gtcgagcaac gctacggcga gttcgacgag
     4921 ctcagcgcgt actcagcgtc gctcgtgctc gcggcgacga gcatcgtgtg tctcgtggtg
     4981 atcaccctca tccggcccaa gtcagacaag aagaagtaac gggaagtacc tcagccatgg
     5041 gcatcagcgt tcgcaacatc aacaagaact tcggcgactt cgtcgctctc ggcgacgtct
     5101 cggtcgacat ccacagcggc aacctcacgg cgctgctcgg gccgtccggc ggcggcaaga
     5161 ccacgctgct gcgcatcatc gccggtctgg agcagccgga ctccggcgtc atcgagatca
     5221 acggcaccga cacgacgtgg ttgcgtcccc aggagcggaa cgtcggcttc gtgttccagc
     5281 actacgcagc gttcaagcac ctgagcgtct accgcaacgt ggcgttcggg ctcgagattc
     5341 gcaaggtccc caaggcacag atccgcacgc gcgtcatgga gctgctcgag ttggtgcacc
     5401 tcgaccagtt cgccgatcga ctgccggcgc agctctccgg cgggcagcgg caacgcatgg
     5461 ccctggcccg agcgctcgcg gtcgagccca acgtgttgtt gttggacgaa cccttcggtg
     5521 cactcgatgc caaggtccgc aaggagttgc gcgactggct ccgtcgcctg cacgacgagg
     5581 tgcacgtcac caccgtgttc gtgacccacg accaggagga ggccctcgag gtctccaacg
     5641 agatcgtcgt gatcaacaac gggcgggtcg agcagatcgg ttcgcccgac gacctgtacg
     5701 accatccggc caacgagttc gtgatgtcgt tcctcggttc gacgacgcgt ctcgacgggc
     5761 acctgctccg tccccacgac gtggacgtct cgacgaccgc tgcgacgggt tcggtctcgg
     5821 ccaaggtcga ccgggtgctg cggatcggat tcgaggtgcg tgtcgatgcc gtgcggccgg
     5881 tggccggcgg tggcgacgaa gtgacgacgg tgcagctgac ccgcaaggag gcgaaccgac
     5941 tcgacctccg tgagggtgac gaggtgtggc tctcacctgc ccccggcgcg acgaccgtga
     6001 tcgcatccgc gccgatcagc accgtcgacg atgacctcga cgacgacgtc ctcgacgtcg
     6061 acgtcgaggc cgaggaggct gccgctcccg tcgggtgaag ccgtccgacg accggctggc
     6121 agccgggcgt gggagactgt cgctgttcgg cggcacggtg agcgtgccgt cggaagcgag
     6181 ggccggggcc accgatgtcg gacgatttcc tgagtgctga tgacctgcgc gcctgggtcg
     6241 aacagggcgt gcacgagccg acggccgagg agcgcctcgc ggaatgggag cgccaggtcc
     6301 gcgccgagga attgcgtatc cgcctcgaag ccgcggagga cgaggcgtgg gaagacgaac
     6361 gtgatcgtcg acaccggcgt cggaaccggc ggatcgtcgt ggccgggtgg ctggtcgtct
     6421 tcgcgctgat cgccggcctg ttggtgcgca cgttcgcgct gtccgacgac cggcgggcgg
     6481 cgtcggcgtg ggcggcactc gatcgggccg acgtcggtac gcgtgtcggg tcgggcgaga
     6541 tgccgagccc gccgatctcg gtgtcgccga ccccgctcgg gacgccgatc gacgtgccgt
     6601 ccaagcgcgg gccgtacgag ttcgtctcga tgcagcccgg cagcgacgca ccggtgacct
     6661 acgacccgtg ccgcccgatt cactacgtcg tgaatcacac cggtcagccg aacgacgccg
     6721 agaacctggt gaccgatgcg atcgagcgga tcagcgaggt gacgggtctg cagttcgtgg
     6781 ccgatgacct gactgacgag gagatcgtcc ctgaccgcca gccgtaccaa cccgatcgct
     6841 atggcgaccg gtgggctccg gtgctgatcg cgtggggcac gcccgatgac acgccgctgc
     6901 tcgccggctc ggttgccggt ttcgggggga gcacgcagta cacggtcacc gaccaggcgg
     6961 ggatctcccg ttcggtgtac gtgaccggcg aggtcgtgct cgacggtccg cagatcgcca
     7021 acatcattcg tctcgatcgc gatggtctcg atggtgcccg cgccgtggtg ctgcacgaac
     7081 tcgcacacgt ggtcgtgctc gatcacgtcg acgacccgac gcagctgatg aatccggtga
     7141 gtcaacccgg cgtcaccgac ttcgctgcgg gcgacctccg aggcctgcgg cagctgggcc
     7201 tgggcgcctg cgtgcccggc atctgaccgg tcgatgagtt ctccgggggc accggtcgta
     7261 cgtgactctg cgcctacgat gccgttgtgc atcgggtagc cggacgcatc gttgtggggc
     7321 tcgtcgtcat cgcgatgtcg atggtcttcg gggccgggcg cgctgctgcg tgcagttgta
     7381 tcgggctcga gtacctcgtg cagaacgagg ccgagttccc gatcgaggcg gccttcgccg
     7441 ggacggtaac ggcagtgccg acgatcgact actcggtacc cgtcgtcacg tggtcgttcc
     7501 ggatcgaccg cgtctaccgg ggcgacgtcg gcaacgtggg cgacacgatc gacgtgcgga
     7561 gctcagcgtc gggcgcgtcg tgcgggttcg agtacatgac ggtgggcgac cggcgcgaca
     7621 tcgcgctgca gcaagacggc ggcgagtgga cgagcggact ctgttcgcag ggcgcggtcg
     7681 gcacgcttgc gccgctggcc ggcgcgtcga ccacggtccc gccccccgac acgacctcgg
     7741 cgacgccgcc gaccatcgag accacgacat ccggtccatc cgagacgacg acgtcgtcga
     7801 ctcccgacga gggctccgtg acgatctcgt cggacgagcc caccggtaac gagggcgggt
     7861 ccagtggtct ggcgatgggt gccggcgcca tcggggcgac cacgctcatt gccggggcga
     7921 tgatcgtggt acggcggcgg cagaacgggg tggcgtccga ttagagcccg cgggcgagcc
     7981 ggtggatctc gtttcggatc cgcttctcgc tgacagggat cggggtgcgc agctgctggg
     8041 cgaacgagct gacgcggaac tcctcgagtg cccaggccag cgcttcgagg tcgtcgctcg
     8101 gagagacggt ctcggccagg ttctcgtact ccgcctcgag cttgcggcag cgttccatga
     8161 gatcagcgtc gcgcttgggg ttgttgggaa ggttggcgag gcggcgctcg atggcccgga
     8221 ggtagcggtc gatgtcgtcg agcttggcga acccaactcc cgtgaggacc ccctcgtaca
     8281 cgagtcggtc gaggtgctcc cggacgtcgg tgaccgcaga ggccatcggc gcagcgacga
     8341 gctggttgag cgtgcggcgg atcgacgagt gcagcgcgat gagcttggct gtcgaggcgg
     8401 tcacggtgac gagggtgtcg tggaggcggt cgcgcacggc gtccacgagg cgctcgaagg
     8461 cagccccggt ccacaccggc ccgccggctt cggcgatcag gtggtcgaga gcgcacccga
     8521 tgatgtcgtt gtaccagtcg accttcggtt gcaccgggct ctgagccatg gcgttcttgg
     8581 tgcgctggtc gaggagtgag tcgacctgtc gggccggcgc cggcatggcc aggcgcacga
     8641 gtcggcgggt gcctgcccac atggttgcga tctgttcgtc ggagctggtg caggcgacga
     8701 tggccgccga gtcgccgtcg tccgcgagcg ccggataggc cgtgatgacg cgaccgttga
     8761 cgttgcgttc gacggttcgg gggaggtcgc cgaacgtcca cgtgcggagt ccgcgttgtt
     8821 cgaggccggt gtcgaccgtc gccacgatcg cggtggcctc ggcgtggacc tgcttggcca
     8881 gcgcccgtag gtcccggccg atcgccaact cccggccgtc ggtgtcgacg atccggaacg
     8941 agggacgcag gtgcttgggg agggcgtcga ggtcgaaggc gtcgacgggg agcggttcgg
     9001 tgatcagccg gttgagttcg cggcgcacgc tctccagcac cggcccgttg tcgggcgtga
     9061 tgcgctcacc gatgaccgca accgtctcgg gcaccggcac gaacagcttc cgcaactgct
     9121 tgggcagcga tcgaatgagc gcgtcgacga ggtcgtctcg gaacccgggc acgttccagt
     9181 cgaaggccgc tggctcgagc tggccgacga tgccggcgtc gatggtgatg gtggcgccat
     9241 cggcctcgct cgtcgggtcg aactcgtagg tgatcggcag gtcgtgcgcg ccgtagtgcc
     9301 acacctcggg gaacgcgtgg ggatcgaccg ccgcatgtgc gtcgtcggcg agcaggtcgt
     9361 cgagcgtgag gtcgaggagc ttgccgttgg cgcgccgctc gtccttccac cactggttga
     9421 acgcggcgcc ggtggtgacc tggtcgggga cccgctgatc gtagaagtcg aagatcgcct
     9481 ggttttcgac gatgagatcg ccgcgccggg cgcgcgcctc gagccgctcg gcctccccgg
     9541 cgacgacggc gttgcggtcc atgaacttgt ggtgggtgtc ccagtcgccc tcgacgagtg
     9601 cgtggtggat gaacaggtcc cgggccatcg ccgggtcgac gcgggcgacc ggcacccggc
     9661 ggcccgacac caacgtgagc ccgtagatcg tgactcgttc gtgggtcatc gccgcgccgc
     9721 gttcgcgttc ccaccagggc tcgctgtatg tgtacttggc gaggtgctca ccgaggcgct
     9781 cgacccacgc cgggtcgatg cgggtggccg tgcgcgccca cagccggttg gtctcgacga
     9841 gttcgccggc catgacccag ttgggtgggt tcttggcgag caccgacccg ggggcgatgg
     9901 tgaaccgggc ctggcgggcc ccgatgtact cgcgggtgcc tcgacggtcg cgctgctcgc
     9961 gtccgtcccg cccgccgtct cggcctcggg gctcgcggcc tttctcgggc gtgtccttca
    10021 tgccgatgtg ggagagcaga ccgctcagca gcgacaggtg cacggcgtcg ggtttggccg
    10081 ggaccgtgtt gggtttgaac ccgagctgtt tggtggcccg gcggagttgc tcgtagatgt
    10141 cctgccactc gcggatgcgg ttgtagttca ggaactcgtt gcggcacatc ttccggaact
    10201 ggttggacga ccgctcacgg cgctgttcgc cgaggtagtt ccagaggttg acgtagctga
    10261 ggaagtcgct gtcggggtgg ttgaaccgga catgctgctg gtcggcctgg gtgcggtggt
    10321 cgaacggacg ctcccgcggg tcctggatcg acatgccggc cgtgatcacc atcacctcgt
    10381 ggaggcaacc gttggtgccg gcttcgatga ccatgcgtcc gagccggggg tcgacgggca
    10441 tgcgcacgag gcggcggccc agcgtggtga gccacttccg ggtgccgtac tggtcggggt
    10501 cgacggcgcc gagctcttcg aggagcgcga tgccgtcgcg gatgttgcgg tcggcgggtg
    10561 ggtcgacgaa ggggaaggac gccacatccc cgaggccgag gttggccatc tggaggatga
    10621 ccgaggcgag attggtgcgg cggatctcgg gttcggtgaa ctcggggcgg gcctcgaagt
    10681 cctcctcgtc gtagaggcgg accgcaatac cgggcccgat acgaccgcag cgaccggacc
    10741 gctggttggc cgatgcctgg gagatgtcct cgatcgggag ccgttgcacc ttggtgcggt
    10801 acgagtagcg ggagatgcgg gcggtgccgg gatcgatcac cgatcggatg cccggcaccg
    10861 tgagggacgt ctcggcgacg ttggttgcga ggacgatccg tcgaccggcg tggcgttcga
    10921 acacccggtg ctgctcggcg gccgacaacc gggcgaacag ggggaacacc tcggtgccgg
    10981 gcaggtgcag gtcgccgagc gctgccgccg cgtcgcggat ctcgcgttcg cccgagcaga
    11041 acacgaggat gtcaccgtcg acttcgcggc ggagctcctc caccgcgtcg caaatgcctt
    11101 cgacctggtc cttcgggccg gcgggatcgt cggggtcgtc gagggggcgg taccggacct
    11161 cgacggggaa ggtgcgtccc tcgacctcga tgatcggagc gccgtcgaag tgctcgctga
    11221 agcgggccgt gtcgatcgtc gccgaggtga tgatcagctt caggtcgggc cggcggggga
    11281 ggagctggcg gagatacccg agcaggaagt cgatgttgag cgaccgctcg tgggcctcgt
    11341 cgatgatgat cgtgtcgtac ttctgcagct cgcggtcgcg ttggatctcg gcgagcagga
    11401 tgccgtcggt catcaccttg atgcgggtgt cggtgccgac ctcgtcgttg aatcgcacct
    11461 tgtagccaac cgcctggccg acctcggaac cgagctcctc ggcgacgcgt tcggcgatcg
    11521 atcgcgcggc gatgcgacga ggttgggtgt gaccgatcca accgccccgg cccgtggtgg
    11581 cgtcctttgc cccgcgcccg gcctcgaggc agagcttcgg gagttgtgtg ctcttgcccg
    11641 aaccggtctc gccggccacg acgagcacct ggtgcgcgcc aatcgccgcg acgaggtcgt
    11701 cgcgtcgttc gctgatcggg agatcaggcg ggtaggtgat ggccggttcc acgagggttc
    11761 aaggctacgg cgtgcgatca tctcccgaga atcgattgtt tcccggagga gtgccgtgca
    11821 gaacgcagtg ttggaagtgg accggacggc gaacgaaacg acccgggtag tgcaccagga
    11881 gttgggcgag ttgggcgacg ggcaggtgcg actgcggatc gaccgattcg cgctgaccgc
    11941 caacaacatc acctacgccg gggtcggcga
//
LOCUS       DAOSOA010000066        11908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5326_length_11908_cov_8.328946, whole genome shotgun sequence.
ACCESSION   DAOSOA010000066 DAOSOA010000000
VERSION     DAOSOA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5326_length_11908_cov_8.328946"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKY13_06145"
     CDS             <1..516
                     /locus_tag="PKY13_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79548.1"
                     /translation="KVAMALHSLISMQYPRDYLGIVGFSEVARVLEAHQLPEVSWDFV
                     YGTNMQHGFVLARQLLARQHGTKQIIMITDGEPTAHINAYGEPEFHYPPIRATVDATL
                     LEVSRCTRDQIRINTFMLDADRGLQHFVERMTELNRGRAFFTSPDTLGDFVLVDFLEQ
                     KRSMLRGRRAG"
     gene            689..970
                     /locus_tag="PKY13_06150"
     CDS             689..970
                     /locus_tag="PKY13_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT79549.1"
                     /translation="MAAMNEEEDHSWQLAANCLGVDPDLFFPERGASTKEAKAVCQGC
                     EVRGDCLEYALANGEKFGIWGGLSERERRRIRRQRALARAAESARSATA"
     gene            complement(947..1552)
                     /locus_tag="PKY13_06155"
     CDS             complement(947..1552)
                     /locus_tag="PKY13_06155"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOT79550.1"
                     /translation="MTDELRSRMRALRRSIPIDVQHLRAQALATTAASLPWWPEVATL
                     GVYRAVAGELDTSVLEQWARGRGIETYVPVLDGRQLRFAPSTPTGTWSQNRFGIDEPD
                     DADTIDAARLDLVVVPAVAVDRAGNRIGMGGGFYDRTFAGRPRSGRPILVAAVHPEQL
                     VERIVTEAWDVAMDAVVLPDEVIVVEGTNRDDRRSGGGGTG"
     gene            1641..1817
                     /gene="tatA"
                     /locus_tag="PKY13_06160"
     CDS             1641..1817
                     /gene="tatA"
                     /locus_tag="PKY13_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOT79551.1"
                     /translation="MQGTDWIIVAVIVIVLFGGTQIPKLARSLGQAQKEFKEGLKEGA
                     AEDTPASDSPADKA"
     gene            complement(1949..2221)
                     /locus_tag="PKY13_06165"
     CDS             complement(1949..2221)
                     /locus_tag="PKY13_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT79552.1"
                     /translation="MNVTDFADTTWMSHGNCAHRDPSMFFPSDGVGVEIARKACEGCP
                     VKTQCLEYAIAERIDHGVWGGCSERERRRIIRSRQTKPADQPVAVG"
     gene            2603..3835
                     /locus_tag="PKY13_06170"
     CDS             2603..3835
                     /locus_tag="PKY13_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT79553.1"
                     /translation="MGGSGAAAVLGPPTAPPPAGPPTGPIGGSFAPGESRPGRAPKEP
                     ARVRPWVSGLAVGALIGAVVASLVAGAIYVASVETGGSVLVADQPRSRTPVTIKGQVL
                     DIGALLDKVRPSVVSIQIDGGMSGEAAGSGIVIDDQGTVLTNAHVVSGAAGSSTAIHI
                     AFSDGEVVPATLVGSFPAQDVALVKTTTVKKTTPAELGSSDALQVGDDVVAIGNALNL
                     GAQPSVTKGIVSALNRQLSAEGEELENLIQTDAAINPGNSGGPLVNALGQVVGINTAI
                     IQNSQSVGFALSIDSVKPLIRDIEDGKGTIDGDSPFLGVETMKVADQSAEILQRFQID
                     VEEGAFVSDVQPGSAADQAGLQPGDVMVSIDGADISSPSDVGAAIQEKSPGDTISIEI
                     QRQGETKTLSAALGRRGG"
     gene            3963..4244
                     /locus_tag="PKY13_06175"
     CDS             3963..4244
                     /locus_tag="PKY13_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79554.1"
                     /translation="MSDIAAATNEAPRASVTVVYLGPVAPHWDVRSDYGEKVVVDEFH
                     RRLNARLMLVPPHDPQFRRNRERVIRDAERENILLAWDPPELADSYSSR"
     gene            complement(4229..4639)
                     /locus_tag="PKY13_06180"
     CDS             complement(4229..4639)
                     /locus_tag="PKY13_06180"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT79555.1"
                     /translation="MTVEAAGGIVVDEAGRVAIVHRPRYDDWSLPKGHLETGETAEDA
                     ALREVFEETGLSCRIIGPAGETHYRDRKGRAKRVRYFSMSVTAGSFLVNDEVDELRWI
                     DESGIDVLSYGFDADLVRSELLERPTRGGDQRDE"
     gene            complement(4636..6717)
                     /gene="ppk1"
                     /locus_tag="PKY13_06185"
     CDS             complement(4636..6717)
                     /gene="ppk1"
                     /locus_tag="PKY13_06185"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOT79556.1"
                     /translation="MDGDPGRFINRELSWLEFDERVLAIAADPSTPALERAKFLAIFS
                     QNLDEFYQVRAAGLLDQVEAGVTDLSPDGLTASQQVSLIARQVAVLTRRADGIFLNEV
                     VPALADAGVRFCQWEELVETDRAFLRAEFDRRIFPILTPLAVDPGHPFPDISNLSLNL
                     AIRVHDPEDGEQRFARLKLPQSLPRFFVTEDKQRLVPLEQLISAHLHRLFNGMVVDEH
                     QTFRVTRNADLDLEEEDAEDLLEAVEVEVRRRRFGKPVRLEVPVAMSEEMVDLLQREL
                     ELEDDEQVHRHDGPLDLSGLWSVYALDRPDLKFATLHPLDARALTAPNGVPADFFHVL
                     RRGDVLVHHPYESFTTSVEEFIGQAANDRHVQAIKVTLYRTSGDSAIVASLIRAAESG
                     KQVVALVELKARFDEENNIEWARRLERAGVHVVYGLVGLKTHTKIAMVARSEGDAVRA
                     YCHVGTGNYNARTARMYEDFGLFTAANDVAIDVQNLFNFLTGYSRDVSYLRLLVAPDH
                     MRTDLVSLIRGERPGPVGHTPRGEGRIVMKMNSLVDPEVIDELYAASRDGVEVDLIVR
                     GICCLRPGVPGLSDRIRVRSIVGRYLEHSRIYRFANGSGPGVPSIHLGSADLMPRNLD
                     RRVEALVTIVNPILLERIDDVLAAQLADDQLAWRLVDSTWRKVPTIAGRECQTEFHDL
                     ARTWASQARNP"
     gene            6916..7803
                     /gene="pstB"
                     /locus_tag="PKY13_06190"
     CDS             6916..7803
                     /gene="pstB"
                     /locus_tag="PKY13_06190"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOT79557.1"
                     /translation="MTTNDRTEARAATPAGAALDSARRPTDEEIPSVVTEAVFETRDL
                     SVLYSGFRAVRNVNLTIRRGEITAFIGSSGCGKSTVLRCLNRMNDLIEGASVEGEVSY
                     RGVNLYDPAVNPVEVRRRIGMVFQKPNPFPKSIWHNIAYGPRVAGVKKKSELDDIVER
                     SLRRAALWDEVKDRLDSSAMGMSGGQQQRLCIARAIASEPEVLLMDEPCSALDPIATA
                     RVEDLMRELRDDYTIVIVTHNMQQAARASDKTAFFGVEVNDDGDRRTGMLVECDTTAK
                     IFSNPSDERTENYITGRVG"
     gene            7805..8521
                     /gene="phoU"
                     /locus_tag="PKY13_06195"
     CDS             7805..8521
                     /gene="phoU"
                     /locus_tag="PKY13_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOT79558.1"
                     /translation="MSDTDLRIEFHESLDELRDDIVRLGALTVETIGKGTAAMLDRDL
                     HAAQALIDGDDVIDTLAISIEESCYRLLALQQPMARDLRFIVCALRFASELERSADLM
                     VNICKASRRIYDVTMSPNVRSLIEDMSIEAASLTRKAIDSFIEADDGLASALDDIDDR
                     LDELQVQFVESIFSSHDSDRLGLRSAVQLALIGRYYERIGDHAVNMGERVTFMVTGWL
                     PEHAAVARLEMKSRRGSPDA"
     gene            8552..9742
                     /locus_tag="PKY13_06200"
     CDS             8552..9742
                     /locus_tag="PKY13_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT79559.1"
                     /translation="MTALVVVLAVAVAVSAGVIATLVTRVRALESAARSTPAPVPAAA
                     EDPTDGSLPRLVAALDALSLGVVVADADEVLGFQNRSAQAILSSRDARTIINRAVREL
                     LVAAVRGEPGEREIDLFGPPKSTYLIRAVPLGNGVAVGGGSVAVIEDVTERRRTDQVR
                     RDFVSNISHELKTPVGAVGLLAETLRDETDLATIRRFSERMSLEVARLSRTIDDLLEL
                     SRIEFADDLFIDETRVGDIVEDALGRFRAAADARGITLKSVGESGIVVAGDRRQITSA
                     LGNLIDNAVKYSPDGATVTVSARVNPSGLALSVADTGDGIPSRDLDRVFERFYRVDRA
                     RSRETGGTGLGLAIVRHVAHNHGGEVSVRSKEGQGSEFTLVLPLGTDVVEPESPVDDS
                     EVSA"
     gene            9739..10437
                     /locus_tag="PKY13_06205"
     CDS             9739..10437
                     /locus_tag="PKY13_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT79560.1"
                     /translation="MNATPVILVVEDEESYIEALNVGLRREGFAVEVARTGPEALQRF
                     DDVDPDLILLDVMLPGVSGVDVCREIRAKSSVPIIMVTAKSSEIDTVVGLEVGADDYV
                     TKPYRLRELVARIRAVLRRRSGAPEQAPRDDVVVVGDVEINVDSREVHLQGDLVPLPL
                     KEFELLLLLMEHAGKVLTRGTIIDRVWGYDYVGDTKTLDVHVKRLRAKIEDDPASPVR
                     LLTVRGVGYKYASN"
     gene            10470..11882
                     /locus_tag="PKY13_06210"
     CDS             10470..11882
                     /locus_tag="PKY13_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79561.1"
                     /translation="MSAADTGAGSDGWQPLTRPPRRLNVDWDAAFPRLALCHLLSVSG
                     ETLVVLSLAGSFFFKVDPAQGREKVILGLLFTMAPFALVGPLIGPMIDRVKGGHRVVI
                     QATMALRVVVAITMMIAVINDSLALFPEAFAMLVLAKTYQIAKAAVVPSTVAGDIELV
                     EANSKLQLLSGLAAIVAGALGGILLQIGPEWVLALTTLTFGAAFVAALRIHPSVTGER
                     EESEIAAGPPPSRSLSDALPPGLAVTAFAMSTLRFLVGLLTFLLAFELRGENQITPLA
                     AIVHWTLQVHRSLVVTTVVPPIDGPPPTWYFGVVIIASVAGGLVGAVLAPPLRRRLAE
                     ERILLGACGLAILAGVAGVILDGLGGYSLMSLGIATAAALGKQAFDAVVQRDVEENDR
                     GRIFARFEARFQITWVVGALVPSILHVPNAAGAILIALGGALATVVMATGVRPVLLNR
                     EVDGRRLGRPYLARNRSSDQ"
BASE COUNT         1842 a         3979 c         4124 g         1963 t
ORIGIN      
        1 aaggtggcga tggcgctgca ctcgctcatc tcgatgcagt atccccgcga ctacctcggc
       61 atcgtcgggt tcagcgaggt cgcccgcgtg ctggaagcac atcagctgcc cgaggtgagc
      121 tgggacttcg tgtacggcac caacatgcag cacgggttcg tgttggcccg tcagctgctc
      181 gcccgtcagc acggcaccaa gcagatcatc atgatcaccg atggggagcc gacggcccac
      241 atcaacgcct acggcgagcc cgagttccac tacccgccaa ttcgggcgac ggtggacgcc
      301 acgctgctcg aggtgtcgcg gtgcacccgc gatcagatcc gcatcaacac cttcatgctc
      361 gacgccgacc gtggcctcca gcatttcgtc gaacgtatga ccgagctgaa ccggggtcgg
      421 gcgttcttca cgtcaccgga cacgttgggc gatttcgtgc tggtcgattt cttggaacag
      481 aagcgctcca tgctgcgtgg gcggcgggcc ggttgaggtc tccgatgggg cggttcgtcg
      541 gcccggcggg cccgggaaac catggttgtg gccggaattc gtcacgattt cgatggtgtc
      601 gttcgcaacg atcgcctctt gcatcgctga ggccgaacga ccatgatgga gggctacacg
      661 tatgtaatta cgcgggggtc cagacgggat ggcagcgatg aacgaggaag aagaccacag
      721 ttggcagttg gcggcgaact gccttggggt cgaccccgat ctcttcttcc ccgagcgtgg
      781 cgcttccacc aaggaagcca aggccgtctg ccagggttgt gaagtccggg gtgactgcct
      841 cgagtacgcc ctcgccaacg gcgagaagtt cggcatctgg ggcggcctga gcgagcggga
      901 gcgtcgtcgc atccgtcgtc agcgcgccct cgcccgcgcc gccgagtcag cccgttccgc
      961 caccgcctga ccgccggtcg tcccggttcg ttccctcgac cacgatcacc tcgtcaggca
     1021 gcacgaccgc atccatcgcc acgtcccatg cctcggtgac gatccgctcg accagttgtt
     1081 ccggatgtac ggccgccacc aggatcggcc ggccggaccg ggggcgaccg gcgaaggtcc
     1141 ggtcgtagaa cccgcctccc atcccgatcc ggttgccggc ccggtcgacg gccaccgccg
     1201 gtacgacgac gaggtcgagc cgggcagcgt cgatggtgtc ggcgtcgtcg ggttcgtcga
     1261 tcccgaaccg gttctgcgac caggtgccgg tcggcgtcga cggcgcgaac cggagctgcc
     1321 gaccgtcgag gacggggacg taggtctcga tgccccggcc gcgggcccat tgctcgagca
     1381 ccgaggtgtc gagttctccg gcaacggccc ggtagacccc gagcgtcgcg acctcgggcc
     1441 accacggcag cgacgcagcc gtggtggcga gtgcctgggc tcgcagatgc tgcacgtcga
     1501 tggggatcga tcgccgcagt gcccgcatcc gtgagcggag ttcgtcggtc acggtggcct
     1561 ccacgatgtc gatcagggtc gtctgtcacc ctgcggggcc cgcgtgatgg gaaccgtggg
     1621 catcaggtta ggatgacgcc atgcaaggaa ccgactggat catcgtcgca gtcatcgtca
     1681 tcgttctgtt cggcggaacg cagatcccga agctggcgcg atcgttgggc caggcgcaga
     1741 aggagttcaa ggagggcctc aaggaaggcg ccgccgagga cacccctgcg tcggactctc
     1801 ccgccgacaa ggcctgacgt ccgaccgctc ggcacgagcc gggaggtgac gcgcccgcgc
     1861 tgacctcggc gaaaacgacg agaccgcccc ggctccgtgg agcgggacgg cctcgtgatg
     1921 gtcgggggag gtgagcgggg aggtgagctc agcccaccgc gacgggctgg tcggccggct
     1981 tggtctgacg gctgcggatg atgcgccgac gctcacgctc ggagcagccg ccccagacac
     2041 cgtggtcgat tcgttcggca atggcgtact cgaggcactg ggtcttgacc ggacagcctt
     2101 cgcaggcctt gcgagcgatc tcgacgccca cgccatcgga cggaaagaac atcgacgggt
     2161 cacggtgtgc acagttgccg tggctcatcc aggtggtgtc ggcgaaatca gtgacgttca
     2221 ttgtgggcct cccgctgccc ggcgtgtcgc cggagcagat tgcgtgtacc cagcatgggc
     2281 tcccggcaga cgcgagccac gctgagcact gagatggctc tcagaacgcg ggccttgcgc
     2341 tgagcccggt cacagcggac ctcatcgcgg tggtgtcggc cgcacctgcc cgggtacata
     2401 ctggcgaggc gatcaaggag cgcacatgtc gaaccaccaa cacgcgccgt caccgttcgc
     2461 gcccccgagc aacacgggcg gcgcgccacc cgttgcccca tcgtcggacc ccgtgtgggc
     2521 cccgccgtcg gagccgtccg gacccccgcc gtacctcgcc ggaccgtcgg ggttcggtcc
     2581 gcccccgtac gctccgcagt cgatgggcgg aagtggcgcc gccgcggtgc tgggcccgcc
     2641 gaccgcgccg ccgcccgccg gcccaccgac cggcccgatc ggtggttcgt tcgcacccgg
     2701 tgagtcgcgt cccggccgtg cccccaagga gcctgctcgt gtccggccgt gggtctcggg
     2761 gctggccgtc ggcgcgctga tcggcgccgt ggtggcgtcc ctcgtcgccg gtgccatcta
     2821 cgtcgcgtcg gtcgagaccg gcggcagcgt gctcgtggcg gatcagcccc ggtcgcgcac
     2881 gccggtgacc atcaaggggc aggtgctcga catcggagcg ctcctcgaca aggtccgacc
     2941 gtcggtggtc tcgatccaga tcgacggtgg catgtccggc gaggccgccg gctcgggcat
     3001 cgtgatcgac gaccagggca cggtgctgac gaacgcgcac gtcgtgtcgg gcgccgccgg
     3061 gtcgagcacc gcgatccaca tcgccttctc cgacggagag gtcgtaccgg cgacgctcgt
     3121 cggcagcttc ccggctcagg acgtggcgct ggtcaagacg accacggtca agaagacgac
     3181 acccgccgag ctcgggtcct ccgatgcgct gcaggtcggc gacgacgtgg tggcgatcgg
     3241 caacgcgctg aacctcgggg cccagccgag cgtgaccaag ggcatcgtct cggcgctcaa
     3301 ccgtcagctg tccgccgagg gcgaggagct ggagaacctg atccagaccg acgccgcgat
     3361 caacccgggc aactcgggag gtccgctcgt caacgcgctg ggccaggtgg tgggcatcaa
     3421 cacggccatc atccagaaca gccagagcgt cggctttgcc ctgtcgatcg actcggtgaa
     3481 gcccctgatc cgcgacatcg aggacggcaa gggcacgatc gacggcgaca gtccgttcct
     3541 gggcgtcgag acgatgaagg tcgccgacca gtcggcggag atcctccagc gcttccagat
     3601 cgacgtcgaa gagggtgcgt tcgtgtcgga cgtgcagccg gggagcgccg ccgaccaggc
     3661 cgggttgcaa ccaggagatg tcatggtgtc gatcgacggt gccgacatct cgtcgccgtc
     3721 cgacgtcggt gcggccatcc aggagaagtc gcccggcgac accatctcga tcgagatcca
     3781 acgtcagggc gagaccaaga cgttgtcggc ggcgctgggt cgccgcggcg gctgacctgc
     3841 cgctcgagcg ggtccgtgct gctgaccggc tgcgctcacg ggtacactcc gttcggcccg
     3901 attcgagctc gggcacccca ccaacgtcga gcgcggccgt ttccgcgcca ccaggagtga
     3961 ccatgtcgga catcgcagca gcaaccaacg aggcgccccg ggcgtcggtg acggtggtgt
     4021 acctgggccc ggttgcaccg cactgggacg ttcggagcga ttacggcgag aaggtcgtcg
     4081 tggacgagtt ccatcgtcgg ctgaacgccc ggctcatgct cgtgccgccc cacgacccgc
     4141 agttccgtcg gaaccgcgag cgggtcattc gcgacgccga gcgcgagaac atcctgctcg
     4201 cctgggaccc gcccgagctc gcggactcct actcgtcgcg ctgatcccca ccgcgggtcg
     4261 gacgctccag caactcggaa cggaccaggt cggcgtcgaa tccgtacgac aacacgtcga
     4321 tgcctgactc gtcgatccac cgcaactcgt cgacctcgtc gttcaccagg aacgatccgg
     4381 ccgtgacgct catcgagaag tagcgcacgc gcttggcgcg acctttgcgg tcccggtagt
     4441 gcgtctcgcc ggccggtccg atgatccggc acgacaaccc cgtctcctcg aacacctcgc
     4501 ggagagcggc gtcctcggcg gtctcgccgg tttcgaggtg gcccttcggg agcgaccagt
     4561 cgtcgtagcg gggacgatgg acgatcgcga cccgtccggc ctcgtcgacg acgatgccgc
     4621 cggccgcctc cacggtcacg ggttgcgggc ctgggacgcc caggttcgag ccaggtcgtg
     4681 gaactcggtc tggcattcgc gtccggcgat cgtcggcacc ttgcgccacg tgctgtcgac
     4741 gaggcgccat gcgagctggt catcggcgag ttgcgccgcc agcacgtcgt cgatgcgttc
     4801 gaggaggatc gggttgacga tcgtgacgag cgcttcgacg cgacggtcga ggttgcgcgg
     4861 catcaggtcg gcggatccga ggtggatgct gggcacaccg gggccggagc cgttcgcgaa
     4921 ccggtagatc cgggagtgct cgaggtaccg gccgacgatg ctgcgcaccc ggatgcggtc
     4981 cgacaggccc ggtacgccgg gccggaggca gcagatccct cgcacgatca gatcgacctc
     5041 gacgccgtcg cgtgacgccg cgtacagctc gtcgatcacc tcggggtcga cgaggctgtt
     5101 catcttcatc acgatccggc cctcgccgcg gggcgtgtgg ccgaccggtc cggggcgttc
     5161 tccgcggatg aggctgacga ggtcggttcg catgtggtcc ggcgccacga ggaggcggag
     5221 gtacgacacg tcgcggctgt agccggtgag gaagttgaag aggttctgca cgtcgatggc
     5281 gacgtcgttc gcagcggtga acaggccgaa gtcctcgtac atccgggcgg tccgggcgtt
     5341 gtagttgccg gtgccgacgt ggcagtaggc gcgcacggcg tcgccctcgg accgggcgac
     5401 catggcgatc ttcgtgtgcg tcttcagccc gaccaggccg tagacgacat gcacgccggc
     5461 gcgctcgagg cggcgtgccc attcgatgtt gttctcctcg tcgaaccggg ccttgagctc
     5521 cacgagtgcc acgacctgct tgcccgattc ggcggcgcgg atcagggagg cgacgatcgc
     5581 gctgtcgccg gacgtgcggt agagcgtgac cttgatggcc tggacgtggc ggtcgttggc
     5641 ggcctgcccg atgaactcct cgaccgaggt ggtgaagctc tcgtaggggt ggtgcacgag
     5701 gacatcgcca cgacggagga cgtggaagaa gtccgcggga accccgttgg gtgccgtgag
     5761 ggcgcgggcg tcgagtggat gcagcgtggc gaacttcagg tcgggtcggt cgagcgcgta
     5821 gaccgaccag aggccggaca gatcgagtgg accgtcgtgg cggtgtacct gctcgtcgtc
     5881 ctcgagctcg agctcgcgct ggagcaggtc gaccatctcc tcgctcatcg cgacggggac
     5941 ctcgaggcgc accggcttgc cgaaccggcg gcgtcgtacc tcgacctcga ccgcttcgag
     6001 cagatcctcg gcgtcttctt cctcgaggtc gaggtcggcg ttgcgcgtga cgcggaacgt
     6061 ctggtgttcg tcgaccacca tgccgttgaa gagtcgatgg aggtgcgccg agatcagctg
     6121 ttcgaggggc accaggcgtt gtttgtcctc ggtcacgaag aagcgcggca acgactgcgg
     6181 cagcttcagg cgggcgaagc gctgctcgcc gtcctcggga tcgtggaccc ggatcgccag
     6241 gttcagtgac aggttggaga tgtcggggaa cgggtggccc gggtcgaccg ccaagggcgt
     6301 gaggatcggg aagatccgtc ggtcgaactc ggcccgcagg aacgcccggt cggtctcgac
     6361 cagctcttcc cactggcaga agcggacgcc ggcgtcggcg agcgccggca cgacctcgtt
     6421 gaggaagatc ccgtcggccc gtcgcgtcag gacggcgacc tggcgggcga tcaggctgac
     6481 ctgctgggat gcggtcagac cgtcggggct caggtcggtg acaccggcct cgacctggtc
     6541 gaggaggccg gcggcgcgca cctggtagaa ctcgtcgagg ttctggctga agatggcgag
     6601 gaacttggct cgttccaatg ccggggtcga cggatcggcg gcgatcgcca ggacgcgctc
     6661 gtcgaactcc agccacgaga gctctcgatt gatgaatcgc ccgggatcgc cgtccacggg
     6721 gtgcatggta ccgacgtgtc aagggggttc cgcagggtgc gaaccccgca gaacctgggg
     6781 acgggggctc gctgcgcagt gttcgaccag cgttcacctc ccgttcaccc ggggtgtggg
     6841 gtcgcgacac cggtcctgcg taccgttctc gccgaaacag gtttcctgcg agactgtttt
     6901 cgagaggaac gtgccatgac gaccaacgac cggaccgagg cccgagcagc gacgcccgca
     6961 ggcgctgcgc tcgactccgc ccgtcgaccc accgacgagg agatccccag cgtggtcacc
     7021 gaagcggtct tcgagacccg cgacctgtcc gtgctctact ccggcttccg ggccgtgagg
     7081 aacgtgaacc tcacgatccg tcgtggcgag atcacggcgt tcatcgggtc gtccggttgt
     7141 ggcaagtcga ccgtgctgcg gtgtctcaac cgtatgaacg acctcatcga gggcgcgtcc
     7201 gtcgagggcg aggtgagcta ccgaggcgtc aacctctacg accctgcggt gaaccccgtc
     7261 gaggtccgcc ggcgtatcgg catggtcttc cagaagccga acccgttccc gaagtcgatc
     7321 tggcacaaca tcgcctacgg cccccgcgtg gccggcgtga agaagaagtc cgagctcgac
     7381 gacatcgtcg agcgttcgct gcgccgtgcc gcgctgtggg acgaggtcaa ggaccggctc
     7441 gactcgtcgg ccatgggcat gtccggcggc cagcaacagc ggctctgcat tgcccgggcg
     7501 atcgcttccg agcccgaggt gctcctcatg gacgagccgt gctcggccct cgaccccatc
     7561 gcgaccgctc gcgtcgagga tctgatgcgc gagctgcgcg acgactacac gatcgtgatc
     7621 gtcacccaca acatgcagca ggcagcccgg gcgtcggaca agacggcgtt cttcggtgtc
     7681 gaggtcaacg acgacggtga ccgtcgtacc ggcatgctgg tcgagtgcga caccacggcc
     7741 aagatcttct cgaacccgtc cgacgaacgc accgagaact acatcacagg gcgcgtcggc
     7801 tgacatgagc gacaccgatc tccgcatcga gttccacgaa tcgctcgacg agctccgcga
     7861 cgacatcgtt cgtctcggcg ccctgaccgt cgagacgatc ggcaagggca cggcggcgat
     7921 gctcgatcgc gaccttcacg ctgcgcaggc gctcatcgac ggcgacgacg tcatcgacac
     7981 gctcgcgatc agcatcgagg agagctgcta ccggctgctc gccctgcaac agccgatggc
     8041 gcgtgatctt cgattcatcg tgtgtgcgct tcgtttcgcg agcgagctcg agcgctcggc
     8101 cgacctgatg gtcaacatct gcaaggcgtc gcgacgcatc tacgacgtga cgatgagccc
     8161 gaacgtgcgc agcctcatcg aggacatgag catcgaggcg gcctcgctca cccgcaaggc
     8221 catcgactcg ttcatcgagg ccgacgacgg tctcgcgtcg gccctcgacg acatcgacga
     8281 ccgcctcgac gagttgcagg tgcagttcgt cgaatcgatc ttcagctcgc acgactccga
     8341 ccggctcggt ctgcggtcgg cggtgcagct ggccctgatc ggccgctact acgagcgcat
     8401 cggcgatcac gccgtcaaca tgggcgagcg ggtcaccttc atggtcacgg gctggcttcc
     8461 cgagcacgct gctgttgccc gcctggagat gaagagccgg cgcggttcgc ccgatgcctg
     8521 attctctcga ctcgacgcgg tcgcgacgat catgaccgct ctcgtcgttg tcctcgccgt
     8581 cgcagtggcc gtctcggccg gggtgattgc gacgctggtg acccgggtac gggcgctcga
     8641 gtctgccgcc cggtccacgc cggcaccggt cccggccgca gccgaggacc cgaccgatgg
     8701 gtcactccca cgtctcgtcg ccgccctcga cgccttgagc ctcggggtgg tcgtcgccga
     8761 tgcggacgag gtgctgggct tccagaaccg ttcggcccag gcgatcctct cgtcgcgcga
     8821 tgcccggacg atcatcaatc gggcggtccg tgagctgctc gtcgccgcgg tgcgtggcga
     8881 accgggcgag cgggagatcg acctgttcgg tccacccaag tccacctacc tgatccgggc
     8941 ggtacccctc ggcaatggag tcgccgtggg cggcgggagc gtggccgtca tcgaagacgt
     9001 gacggaacgc cggcgcaccg accaggtgcg gcgggatttc gtgtcgaaca tcagccacga
     9061 gttgaagacc cccgtggggg ccgtcgggct gctggcggag acccttcgcg acgagaccga
     9121 cctggcgacg atccggcggt tctccgagcg gatgagcctc gaggtcgctc gcctgtcgcg
     9181 aacgatcgac gatctgctcg agctgagccg tatcgagttc gcggacgatc tcttcatcga
     9241 cgagacccgg gtgggcgaca tcgtcgagga cgcgctcggg cgcttccgag ccgccgccga
     9301 cgcccggggc atcaccttga aatccgtcgg cgagtccggc atcgtcgtcg ccggcgaccg
     9361 tcgacagatc acgagcgcgc tgggcaacct gatcgacaac gccgtgaagt acagccccga
     9421 cggagccacc gtgacggtct cggcccgagt gaatccttcg ggcttggcac tgtcggtcgc
     9481 cgacaccggc gacggcatcc cgagtcgaga cctcgatcgg gtgttcgaga ggttctaccg
     9541 ggtcgaccgt gcccggagcc gcgagacggg cgggacgggc ctcgggctcg ccatcgttcg
     9601 tcacgtggca cacaaccacg gtggagaggt gtcggttcgt tccaaggagg ggcaagggtc
     9661 ggagttcacc ctcgtcctgc cgttggggac cgacgtggtc gaaccggaga gcccggtcga
     9721 tgattccgag gtgagcgcgt gaacgcaacg ccggtgattc tggtggtcga ggacgaagag
     9781 tcctacatcg aggccctgaa cgtcgggctc cgacgtgagg gcttcgccgt ggaggtggcc
     9841 cgcacgggcc ccgaagccct gcaacggttc gacgacgtcg atcctgacct gatcctgttg
     9901 gacgtgatgc tgcccggcgt ctcgggcgtc gacgtgtgcc gggagatccg ggcgaagtcg
     9961 tccgtgccga tcatcatggt gacggccaag tcgtcggaga tcgacaccgt cgtcggtctc
    10021 gaggtcggcg ccgacgacta cgtgacgaag ccgtaccggt tgcgcgagct ggtcgcccgc
    10081 atccgtgccg tcctccgtcg gcgaagcggt gcgcccgagc aggcgccccg cgacgacgtg
    10141 gtggtggtcg gtgacgtcga gatcaacgtc gacagtcgcg aggtccacct ccagggcgat
    10201 ctcgttcccc tgccgctcaa ggagttcgag ctgctcctgc tcctcatgga gcacgccggc
    10261 aaggtcctca cccggggaac gatcatcgac cgggtgtggg ggtacgacta cgtcggtgac
    10321 acgaagaccc tcgacgtcca cgtgaagcgg ctgcgggcga agatcgagga cgatcctgcc
    10381 tcgccggtga gactgctcac cgtccgcggc gtgggataca agtacgcgtc gaattagtct
    10441 gaccgggtga tcgacccgga ccgtcccgca tgagcgctgc ggacacgggg gcggggtccg
    10501 acggctggca gccgctcacg cgcccgccgc ggcgcctcaa cgtcgactgg gacgcggcgt
    10561 tcccccggct cgcgttgtgc cacctgctct cggtgtcggg cgagaccctc gtggtgctca
    10621 gcctcgccgg ttcgttcttc ttcaaggtcg atccggccca gggccgcgag aaggtcatcc
    10681 tcggtctgct gttcacgatg gcgccgttcg cgctggtcgg tccgctcatc gggccgatga
    10741 tcgaccgggt gaagggcggc catcgcgtcg tcatccaggc gacgatggcg ctgcgtgtcg
    10801 tggtcgccat cacgatgatg attgcggtca tcaacgacag cctggcgctg ttccccgagg
    10861 cgttcgcgat gctcgtgctg gcgaagacgt accagatcgc caaggcggcc gtggtgccgt
    10921 caaccgtcgc gggtgacatc gagctggtcg aggcaaactc caagctccag ctgctgagcg
    10981 ggctcgcggc gatcgtcgcc ggtgcgctgg gcggcatcct cttgcagatc ggccccgagt
    11041 gggtgctggc gctcacgacc ctgacgttcg gagcggcgtt cgtcgccgcg ctgcgaatcc
    11101 atccatcggt gacaggggag cgcgaggagt cggagatcgc cgcgggccca ccaccgtcgc
    11161 gctcgttgtc cgacgccctc cctcccggtc tcgccgtcac cgcgttcgcg atgtcgacgt
    11221 tgcgattcct cgttggcctc ctcacgttcc tcctggcctt cgagttgcgc ggtgagaacc
    11281 agatcacccc gctcgcggcg atcgtccact ggaccttgca ggtgcaccgg agcctcgtcg
    11341 tcacgaccgt cgttccgccg atcgacggtc cgccgccgac ctggtacttc ggtgtcgtca
    11401 tcatcgccag cgtcgccgga ggtctcgtgg gcgccgtcct cgccccgccg ttgcgccgcc
    11461 gcctcgccga ggaacggatc ctgctcggcg cctgtggtct cgcgatcctc gccggggtcg
    11521 ccggcgtgat cctcgacggg ctgggcggct attcgctgat gtcgctcggc atcgccacgg
    11581 cggcggcgct cggcaagcag gcgttcgacg ccgtcgtcca gcgcgacgtc gaggagaacg
    11641 atcggggccg aatcttcgcc cggttcgagg cgcgattcca gatcacgtgg gtcgtcggcg
    11701 cgctcgtgcc gtcgatcctg cacgtcccga acgccgccgg tgcgatcctg atcgccctcg
    11761 gcggcgcact ggcgacggtc gtgatggcga ccggggtccg cccggtgctc ctcaaccgcg
    11821 aggtcgacgg tcggcgcctg ggccggccct acttggcaag gaaccgttcg tcagatcagt
    11881 gaaccccggg ggatcgctct gcgctatg
//
LOCUS       DAOSOA010000067        11906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5327_length_11906_cov_6.925829, whole genome shotgun sequence.
ACCESSION   DAOSOA010000067 DAOSOA010000000
VERSION     DAOSOA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11906
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5327_length_11906_cov_6.925829"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PKY13_06215"
     CDS             complement(<1..731)
                     /locus_tag="PKY13_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT79562.1"
                     /translation="MSGIDSAPRERRVVGGHGIKLSVVERGDADRSTVVLVHGYPDQQ
                     HVWDPVAERLAANHHVVTYDVRGAGRSGVPANVDGYRLDRLVADFVAVVDAVAPGRQV
                     HLVGHDWGSIQCWEIVCTAGVSDRVASFTSISGPSLDHVGQWVRRRTSFGPTIGQLVG
                     QAAKSWYVVALQSPITPVLWRRLIWRRLPPVLARTEGVEADDEWPGSTLAEDGANGTN
                     LYRANIHERVRRPRPRRTSVPTQVIA"
     gene            803..1996
                     /locus_tag="PKY13_06220"
     CDS             803..1996
                     /locus_tag="PKY13_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOT79563.1"
                     /translation="MRALATAALLATATFATACAGATVDSVADDAVEVATDRPDPQVA
                     TTTTTPPSTTTTTIDTIPSDQRVMSKAQVITAGPLTPKSVEASNGGLFFAQNMIYSHT
                     VTVYDRSFALVKQIPDTVNLAEWGFEGHPGESKGGPVEMAFSHDGTKAYVSNYQMYGA
                     GFDNPGDDKCNKGGWDNSFLYRIDTASLAIDQVIEVGPVPKYVAVTPDDSTVLASNWC
                     GYDLSVVDAATGKETKRIDIGRFPRGIAVMPDSTTAFVAAMGTKDIAVVNLASGAVEW
                     IKGVGQGPRHLVLDPKGQFLYATLNGEGKVAKIDVATRTVVAKVASPSQPRSMTIAPD
                     GGSLYVVNYDSDAMSKIRTSDMQQIQRVPTGHHPIGITYDKVDHRVWVACYSGSLYVF
                     DDAAP"
     gene            2029..>2252
                     /locus_tag="PKY13_06225"
     CDS             2029..>2252
                     /locus_tag="PKY13_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT79564.1"
                     /translation="MLIAQISDPHVTTEGTAIRALVDTPQRLVDALDTCAGLPRVPDV
                     ILLTGDLVNDGTPAEYELLAAALASAPCPVL"
     assembly_gap    2253..2352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2353..3065
                     /locus_tag="PKY13_06230"
     CDS             <2353..3065
                     /locus_tag="PKY13_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT79565.1"
                     /translation="AEYELLAAALASAPCPVLPIPGNHDDAARLRATFATLDCCPVEL
                     PDPTGPFHYVVDDHPVRLVAFDTTDAGNHSGLVTTEGAQWLDRTLALRPEAPTIVFMH
                     HVPYATGAWWFDYNGVAGAEFLREVLANHTQVVRVVAGHVHRTSSTQWGRLTLSAAPS
                     TAYLSGTGVAGGPPVIIDQRSAVQLFSWDGDAILASEADLPAPGATLDLRHMNPNWDA
                     YEQAAKAQGPISKERFGG"
     gene            3101..3904
                     /locus_tag="PKY13_06235"
     CDS             3101..3904
                     /locus_tag="PKY13_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOT79566.1"
                     /translation="MDISTFRVKRSDTVDLTKIPTNAPKGISKSEARERIAQLTTELE
                     ELQYLLYADHHHKVLAVIQATDTGGKDGTIRNVFGACNPQGVRVTSFKAPSEDELAHD
                     YLWRVHPEVPGTGELVVFNRSHYEDVLIVRVHDLVPPSRWKKRYQHIVDFERLLTDEG
                     TTIVKFYLHISKGEQKERLQARLDDPTKNWKFAEGDLAERKRWDDYQTAFTDMLNSTS
                     TSEAPWYVIPADNKWYRDLVVAEIMVQTLRGLDMTFPPAPPNLAGIVVE"
     gene            complement(3920..5722)
                     /locus_tag="PKY13_06240"
     CDS             complement(3920..5722)
                     /locus_tag="PKY13_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOT79567.1"
                     /translation="MASSPEGPGWWQASDGQWYPPESHPNYRPPAAPPTAPPTEQYQL
                     PPTQPTLQYQAPPAAPPYQAPTAPPAYGQPNPYGQPPQGPPPGQYVGGAYGAPPPAAP
                     PASGKGKGILIAAVVVAVLLIAGVLVAVLAGGSDDDSADGGPEVIVGGRDTGSTTGGQ
                     VGTDPDKSIKEDAPDATSELDIVGPSNGAENAVISAAIVDIQSFWTDEMPDAYGADYQ
                     PVEGGFYSWSPDEELPPCVDSPDNISGNAFYCGQADVVAWDDTDLIPHLYETYGDLSV
                     AIVFAHEWGHAIQARVGMEGATVTLEQQADCFAGAWVQHVQSGGSDFFTADGPALDQA
                     LAGFLEIADTPGTSAMDENAHGSAFDRINSFQEGLEGGAATCSGYSDETVGPRLVELE
                     FQSQEDLDAGGNAPYEDIIELSTSDLEDYWSKIGQQDFDTTWTTLSPAQAFDGTSGES
                     PPCGDSDTSGYTLFYCAEDNFVAYDDVGLFPEVYDSLGDFAVGALYGSQYSLAAQNQF
                     GIAPDDARDQNLMADCMTGSWAASIFLQDRITDQDEVLRLQLSPGDFDEAIKVLLALG
                     SREEDGGTQGTGFERVTAFRKGVVNGVEACTSGG"
     gene            5871..6173
                     /locus_tag="PKY13_06245"
     CDS             5871..6173
                     /locus_tag="PKY13_06245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOT79568.1"
                     /translation="MIVISGTIVIDPAHLDAATTAIAELETQTRAEPGNIDYGFWLSH
                     SEPGTLHVFEEWADEDALNAHMGSEHMAAFLGAAGSFGISGTSIDRYDVTTKTKFM"
     gene            6182..6832
                     /gene="msrA"
                     /locus_tag="PKY13_06250"
     CDS             6182..6832
                     /gene="msrA"
                     /locus_tag="PKY13_06250"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOT79569.1"
                     /translation="MLFSRAKTTMVEPDAALGGRDQAIVTPEPHFVSGRSLVAPFPEG
                     HEQLVVGLGCFWGAERLFWQTEGVWVTAVGYAGGYTPNPTYEEVCSGKTGHTEVVLVD
                     FDPAVISLEQVLAAFWEGHNPTQGMAQGNDVGTQYRSAVYVTSAEQRAAAERTRDAFQ
                     AKLDAAGYGSITTEIAEAGPFYYAENYHQQYLAKNPGGYCGLGGTGLSCPIGLATD"
     gene            6943..7350
                     /locus_tag="PKY13_06255"
     CDS             6943..7350
                     /locus_tag="PKY13_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79570.1"
                     /translation="MTEESDAPTGARWNRRWPERAGWALTGAVVTAIALSICGSMHAG
                     VSRSWYPPGEIDDPRVQACSVFLRRPLPSLALLGWDDAEMWVGPTADRGTVISVSDDH
                     LDDIKVTWDATGATITESTGVRHHVAARICPDQ"
     gene            complement(7567..7746)
                     /locus_tag="PKY13_06260"
     CDS             complement(7567..7746)
                     /locus_tag="PKY13_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HOT79571.1"
                     /translation="MAKFEVGDRVIITEGDFKGDRGTITDKNLIGDGIKVALEEDGKE
                     IKTEEGHVSLLERED"
     gene            complement(7958..9367)
                     /locus_tag="PKY13_06265"
     CDS             complement(7958..9367)
                     /locus_tag="PKY13_06265"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOT79572.1"
                     /translation="MLSVVAVVTTACPPETGTPEETTTTTSTTTTSTTTSTTSTSTTT
                     STTTTTLPPDDPNGRYVATTGTDAGDCSASAAPCLTVNYAVGQAVSGNTVYVAAGTYA
                     EVVAPNKNLTFKGANAGIAAGVSPGVRSAESIVKGFRNPGNPGTTSYTTTIDGFRVDP
                     QGDTALISATLQPLVWLRGGATVVENNVFSGGSFVSNCTYTCTTMTDSAFTVQSGSVT
                     FSNNTIENFRRPVTINQALGAPATSATVSGNAFTGITSRALALAGSTGVQMGGQTVTG
                     NTFDATGRTSPSSPAGITISNGGNVISGNTFTGLASGVYLDLCKKFVTNNNAITGNTF
                     TGNNGGVNISVNTDGGQCVSSATEGSGGWVAGGGRINGMTITGNNFSGNTSYAIRHAA
                     FNWGFFTATAPTSTGPVDATCNFYGTPAAPNYASYSYPPGTVAANPDGVIGTAAPETS
                     VNSTPWRTAAAPAGACDGV"
     gene            9545..10282
                     /locus_tag="PKY13_06270"
     CDS             9545..10282
                     /locus_tag="PKY13_06270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOT79573.1"
                     /translation="MPVALFLHHDANSSSGLLGEIVTELGYEVRDHHICTELASGVAT
                     GPLPTIEGVDLLIPLGSRWSVYDTENIGSWIDDEVELVREADRRGVPVIGVCFGGQLV
                     ATALGGSVLPAPFEEIGWIEIEPVADVEHPIPTGPWFQWHLDVFTPPADATVLATSPA
                     GAQAFRLRDNLALQFHPEVDTAVLQEWMITDRDQLRECGVDPDELLERTARETAKART
                     RAEAVVRAFLDSVDYAAAPTGASLDVD"
     gene            complement(10240..>11906)
                     /locus_tag="PKY13_06275"
     CDS             complement(10240..>11906)
                     /locus_tag="PKY13_06275"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOT79574.1"
                     /translation="GIFTKAADVGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDCA
                     GMAADLFESFGVTLVASIILGVSAFSAGGLGLEGTEAAKGLIFPLAVVALGLVASVIA
                     IYMVKANESENDALKPINRGLNIASVLALGGTAVVAFGYVGNPEGSEISNPALRMFLA
                     VLAGVVLGQAASRITQYFTSSQFKPVKDIANSGRTGPATVLLSGLSSGLESAVWAAVA
                     ICVAIGVALGLGDGNSQFGLYLIALTGIGMLSTTAIVVAEDTFGPIADNAQGIAEMSG
                     VFEGRTEEILDGLDTVGNTTKAVTKGFAIGTAVIAAVALFASFVETIGEGTVINIAEP
                     KTFIGAIIGGAVVFLFSSLAILAVGRTAGVVVEEVRRQFREKPGIMDYTEKPDYGPVI
                     DICTSAALRELITPALLAVLVPVAVGFGLGPYALGGYLIAAIVVGQLMANFLSNAGGA
                     WDNAKKYIEEGNHGGKGSDVHKAVVIGDTVGDPFKDTAGPAINPLIKVMNLVSLLVLP
                     AVLSLSDDGIRFVIAGGAVVVLIGAVLFSKRSGQSTSSEAPVGAAA"
BASE COUNT         1994 a         4173 c         3807 g         1832 t
ORIGIN      
        1 gcgatcacct gggtcggcac cgacgtccga cgcggtcgcg gtcgtcggac acgctcgtgg
       61 atgttggccc ggtagaggtt cgtgccgttc gctccgtcct cggcgagtgt cgaaccgggc
      121 cactcgtcat ccgcttcgac cccttcggtt cgagccagca ccggcgggag ccgtcgccaa
      181 atcagacgcc gccacaacac cggtgtgatc gggctctgca gcgccaccac gtaccacgac
      241 ttggcggcct ggccgacgag ctgaccgatc gtcggcccga acgaggtgcg tcgacgcacc
      301 cactgaccca cgtggtccag cgacggcccg gagatcgacg tgaatgatgc gacccggtcc
      361 gaaactcctg cggtgcagac gatctcccaa cactggatcg acccccagtc gtgtccgacg
      421 aggtgaacct gacgcccggg cgccaccgcg tcgaccacag cgacgaagtc ggcaaccaac
      481 cggtcgagtc ggtacccgtc gacgttcgcc gggaccccgg accgaccggc gccgcgcacg
      541 tcgtacgtca cgacgtggtg attggcagca agacgttcgg cgaccggatc ccatacgtgc
      601 tgttggtcgg ggtagccgtg cacgaggacg acggtcgatc ggtcggcatc tccgcgctcg
      661 accaccgaga gcttgatgcc gtgaccaccg acgacgcgtc gttcacgagg ggcgctgtcg
      721 attccgctca cggtcgtcac tcttgcagct cacctgaggg ccgtcatcac caccggcgag
      781 ccccgcctga cagactgtcg aggtgcgtgc cctcgccacc gcagccctcc tcgcgaccgc
      841 aacgttcgcc actgcctgcg ccggggcaac cgtcgactcg gtcgccgacg acgcggtcga
      901 ggtcgccacc gaccgtcccg acccgcaggt cgccacgacc accaccactc ccccgtcgac
      961 cacgacaacg acaatcgaca cgatcccgtc cgaccaacgg gtcatgtcca aggcgcaggt
     1021 catcaccgcc gggccgctca cccccaagtc ggtcgaagcc tccaacggcg ggctcttctt
     1081 cgcccagaac atgatctaca gccacaccgt cacggtgtac gaccggtcgt tcgccctcgt
     1141 gaagcagatc cccgacaccg tgaacctcgc cgagtggggc ttcgagggcc accccggcga
     1201 gtcgaagggc gggccggtcg agatggcgtt cagccacgac ggcaccaagg cctacgtatc
     1261 gaactaccag atgtacggcg ccggcttcga caaccccggc gacgacaagt gcaacaaggg
     1321 cggctgggac aactcgttcc tctatcgcat cgacacggcg agcctggcga tcgaccaggt
     1381 gatcgaggtc ggccccgtcc ccaagtacgt cgctgtcaca cctgacgact ccaccgtgct
     1441 ggcgagcaac tggtgcggct acgacctcag cgtcgtcgat gccgccaccg gcaaggagac
     1501 caagcgcatc gacatcggcc gctttcctcg cggcatcgcc gtgatgcccg attccacgac
     1561 cgcgttcgtc gccgcgatgg gcacgaagga catcgccgtc gtgaacctgg catcgggcgc
     1621 cgtcgagtgg atcaagggtg tgggccaagg tccccgccac ctcgtcctcg accccaaggg
     1681 ccagttcctc tacgcgacgc tcaacggcga gggcaaggtc gcgaagatcg acgtggccac
     1741 gcgaaccgtc gtcgccaagg tcgcatcgcc gagccagccg cgatccatga cgatcgcgcc
     1801 cgacggcggc tcgctctacg tcgtcaacta cgactccgac gcgatgagca agatccgcac
     1861 ctccgacatg cagcagatcc aacgcgtccc caccggccac caccccatcg gtatcaccta
     1921 cgacaaggtc gaccaccggg tctgggtcgc ctgctactcc ggcagcctgt acgtgttcga
     1981 cgacgctgcg ccgtgacgcc gactccgtcg tacggcaaga ctcacgacat gctgatcgcc
     2041 cagatctccg acccccacgt cacgaccgag ggcaccgcga tccgggcact cgtcgacacc
     2101 ccccagcggc tggtcgacgc gctcgacacg tgcgcgggcc tgccccgggt gcccgacgtg
     2161 atcctcctca ccggcgacct cgtcaacgac ggaacgccgg ccgagtacga gctgctcgca
     2221 gccgccctcg cgtccgcccc ctgccccgtc ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nncggccgag tacgagctgc tcgcagccgc cctcgcgtcc gccccctgcc
     2401 ccgtcctgcc gatccccggc aaccacgacg acgccgcccg gctccgggca acgtttgcca
     2461 cgctcgactg ttgccccgtc gaactccccg accccaccgg cccgttccac tacgtcgtcg
     2521 acgaccatcc cgtacgactc gtcgctttcg acacaaccga cgccggcaac cacagcggcc
     2581 tcgtcaccac cgagggcgcc cagtggctcg atcgcaccct ggcgctccgc cccgaggcgc
     2641 cgacgatcgt gttcatgcac cacgtcccgt acgcaaccgg cgcatggtgg ttcgactaca
     2701 acggcgtcgc cggcgccgag ttcctgcgcg aggtcctcgc gaaccacacc caggtcgtcc
     2761 gggtcgtcgc cggccacgtg caccgcacct cgtccaccca atggggccgc ctcacgctca
     2821 gcgccgcgcc gtcgaccgcc tacctgagcg gcaccggcgt cgccggcggc ccgccggtca
     2881 tcatcgacca gcggtcggcg gtgcagctgt tctcgtggga cggcgacgcg atcctggcgt
     2941 cggaggccga ccttcccgct cccggagcga cgctcgacct ccgtcacatg aacccgaact
     3001 gggacgcgta cgagcaggcg gcaaaagcgc aggggcccat ctcgaaggaa cgcttcggcg
     3061 gctaggagcg accgggcgtt ccgcgcggca cacttgcccc atggacatct ccacgtttcg
     3121 ggtgaagcgc tccgacaccg tcgacctcac caagatcccg acgaatgcgc ccaagggcat
     3181 ctcgaagtcc gaggctcgcg agcgcatcgc acagctcacc accgagctcg aggaactgca
     3241 gtacctgctc tacgccgacc accaccacaa ggtgctcgcc gtcatccagg ccaccgacac
     3301 gggcggcaag gacggcacga tccgcaacgt gttcggcgcg tgcaaccccc aaggcgttcg
     3361 ggtcacgtcg ttcaaggcac cgtcggagga cgagctcgcc cacgactacc tgtggcgggt
     3421 ccaccccgag gtgcccggta ccggcgagct cgtggtgttc aaccgcagcc actacgagga
     3481 cgtgctgatc gttcgggtcc acgacctcgt cccaccgtca cggtggaaga agcggtacca
     3541 acacatcgtc gacttcgagc gactgctcac cgacgagggc acgacgatcg tgaagttcta
     3601 cctgcacatc tcgaaaggcg agcagaagga acggctgcag gccaggctcg acgacccgac
     3661 gaagaactgg aagttcgccg agggtgacct ggccgaacgc aagcggtggg acgactacca
     3721 gacagcgttc accgacatgc tgaactcgac ctccacgtcg gaggccccgt ggtacgtcat
     3781 cccggccgac aacaagtggt accgcgacct cgtcgtcgcc gagatcatgg tccagacgct
     3841 gcgcggcctc gacatgacgt tcccgccggc accgccgaac ctcgccggca tcgtcgtcga
     3901 gtaaccgacc gcctccggct cagccgccgg aggtgcaggc ctccacaccg ttgaccacgc
     3961 cttttcggaa ggcggtcacg cgctcgaacc cggtgccctg ggtcccaccg tcctcttcgc
     4021 gcgatcccag cgcgagcagg actttgatgg cctcgtcgaa gtcgcccggc gacaactgga
     4081 gacgcaggac ctcgtcctgg tcggtgatgc ggtcctgcaa gaagatgctc gccgcccacg
     4141 atcccgtcat gcagtccgcc atcaggttct ggtcacgagc atcgtcgggt gcgatcccga
     4201 actggttctg cgctgcgagc gagtactggc ttccgtagag cgctccgact gcgaagtcac
     4261 ccagcgagtc gtagacctcg gggaacagcc cgacgtcgtc gtaggcgacg aagttgtcct
     4321 cagcgcagta gaagagcgtg tagccgctgg tgtcggagtc accgcacggc ggggactcac
     4381 cggaggtgcc gtcgaacgct tgcgccgggg agagcgtggt ccaggtggtg tcgaagtcct
     4441 gctggccgat cttggaccag tagtcctcga ggtcggaggt cgacagttcg atgatgtcct
     4501 cgtacggtgc gttgccgccg gcgtccaagt cctcctgtga ctggaactcc agctcgacga
     4561 gccgagggcc gaccgtctcg tcgctgtagc ccgaacaggt cgcggcgccg ccctcgaggc
     4621 cttcctggaa cgagttgatg cggtcgaacg cgctgccatg tgcgttctcg tccatcgccg
     4681 acgtacccgg cgtgtcggcg atctcgagga aaccggcgag ggcctggtcg agcgccggac
     4741 cgtcggcagt gaagaagtcg gagccgccgg actgcacgtg ctgcacccag gcgccggcaa
     4801 agcagtccgc ctgctgttcg agcgtgacgg tcgcaccctc catgcccacc cgcgcctgga
     4861 tcgcgtgacc ccattcgtgg gcaaagacga tcgccacgga gagatcgccg taggtctcgt
     4921 agaggtgcgg gatgagatcg gtatcgtccc aggcgaccac gtcagcctgc ccgcagtaga
     4981 acgcgttgcc cgagatgttg tcgggcgaat ccacgcacgg cggcaattcc tcgtcaggac
     5041 tccacgagta gaagccgcct tcgaccggct ggtagtccgc cccgtacgcg tcgggcatct
     5101 cgtcggtcca gaagctctgg atgtcgacga tcgccgccga gatcaccgcg ttctcggcgc
     5161 cgttcgacgg cccgacgatg tcgagctcgc tggtcgcgtc cggcgcgtct tccttgatgc
     5221 tcttgtcggg gtcggtgccg acctggccgc cggtggtgct cccggtgtcc cgtccgccga
     5281 cgatcacctc ggggccaccg tcggccgaat cgtcatcgga cccgccggcg aggactgcaa
     5341 ccagcacacc ggcgatcagc aacacggcga ccacgaccgc agcgatgagg atgcccttgc
     5401 ccttccccga agcgggcggt gccgccggcg ggggcgcgcc gtaggcacct ccgacgtact
     5461 ggccgggcgg cggaccctgt ggaggctggc catacgggtt gggttgccca tacgcgggcg
     5521 gtgccgtcgg cgcctggtag ggcggcgccg cgggaggtgc ctgatactgc agcgtcggct
     5581 gggtcggggg tagctggtac tgctcggtcg gaggcgcagt cggaggcgcg gccggcggcc
     5641 ggtagttggg atggctctcg gggggatacc actgcccatc ggacgcttgc caccaaccag
     5701 gaccctcggg ggaagaagcc atcgcggaag gctacttccg acaatcgaca agacctacct
     5761 cttcctcatg acgatcctgc gatccgggag tggggtcgac tgcctctaca gtgcggagat
     5821 tcgccgcccg tctcccgacg ggcgactcac acacggggga aaccacgcac atgatcgtca
     5881 tctcgggcac catcgtcatc gacccggcac acctcgacgc ggccaccacg gccatcgccg
     5941 aactcgagac ccagacccgc gccgaacccg ggaatatcga ctacggattc tggttgtccc
     6001 acagcgagcc cggaaccctc catgtgttcg aggagtgggc ggacgaggac gccctcaacg
     6061 cacacatggg ctccgagcac atggccgcct tcctcggggc ggccggctcc ttcggcatct
     6121 ccggtacgag catcgaccgg tacgacgtca ccaccaagac caagttcatg tgaggtaacc
     6181 catgttgttc tcccgagcca agaccacgat ggtcgaaccc gacgccgcgc tcggcggtcg
     6241 ggaccaggcg atcgtcacgc ccgagccgca cttcgtgagc ggccggtcgt tggtggcgcc
     6301 gtttcccgag ggccacgagc agctcgtggt cgggctcggg tgcttctggg gtgccgagcg
     6361 cctgttctgg cagaccgagg gagtgtgggt gaccgccgtc ggctacgccg gcgggtacac
     6421 ccccaacccc acctacgaag aggtgtgcag cgggaagacc ggtcacaccg aggtcgtgtt
     6481 ggtcgatttc gacccggcgg tcatcagcct cgagcaggtg ttggcggcgt tctgggaggg
     6541 ccacaacccc acccagggca tggcccaggg caacgacgtc ggcacgcagt accgctcagc
     6601 ggtctacgtg acgagcgccg agcagcgggc cgccgccgaa cgcacccgcg acgcgttcca
     6661 ggccaagctc gacgccgccg gctacggatc gatcacgacc gagatcgccg aagccggacc
     6721 cttctactac gccgagaact accaccagca gtacctcgcc aagaaccccg gcggctactg
     6781 cggactcggc ggcaccggac tcagctgccc catcgggctc gccaccgact gagcctcgag
     6841 agcgaaccgc gtgcagatcg cgcccaatga gggccgaatc tgcacgcggt tcgtgagtca
     6901 cgctgagctg gcccggtcgg tcgactcacc tcccctgacg ccgtgaccga ggaaagcgac
     6961 gcgccgaccg gggctcgatg gaatcgtcgc tggccggagc gagccggctg ggcgctgacc
     7021 ggcgccgtcg tcaccgcgat tgccctcagc atctgcggct cgatgcacgc cggggtgagt
     7081 cgcagctggt acccgcccgg cgagatcgac gacccgcggg tccaggcgtg cagtgtcttc
     7141 cttcgacgcc cactcccgag cctcgccctc ctcggatggg acgacgccga gatgtgggtg
     7201 ggcccaacgg cggacagggg cacggtcatc tcggtgtcgg acgatcacct cgacgacatc
     7261 aaggtgacct gggatgccac tggcgccacg atcaccgaat cgacgggcgt ccgacaccac
     7321 gtagcagccc ggatctgccc cgaccagtag aaggacgaac cggcgccgac agcagtcgac
     7381 cacgcttgcg cttcatccgg ggccaccgct agcagtacct cgccaagaac cccggcggct
     7441 actgcggcct cggtggcacg gggctcagct gcccaatcgg tctcgccacc gactgacctc
     7501 caagagcgaa ccgcgtgcag atcacgcccc ttgagggccg aatctgcacg cggttcgtga
     7561 ggtgtttcag tcctcgcgct cgaggaggct cacgtggccc tcttcggtct tgatctcctt
     7621 gccgtcctcc tcgagggcga ccttgatgcc gtcgccgatg aggttcttgt cggtgatcgt
     7681 cccgcgatca cccttgaaat caccctcggt gatgatgacc cggtcgccga cctcgaactt
     7741 cgccatgatc ctgctccttc tcggcaccgt ggggaaccgg cgcaccatcg acgctatcgc
     7801 cggagggccc ggaaactgcc tgaccagggc gacggtttcc cgacgcccgt cgccggccgc
     7861 ggtcccgcac gacgatgccg tccggacgcg acgtcacccg gaccacgctc ccgaacggag
     7921 cgcggaccgg gtgacggtca ggatgactcg gttgcgatca gacgccgtcg caggcgcccg
     7981 caggcgcagc tgccgtacgc cacggggtgg agttcaccga ggtctcgggg gcggccgtgc
     8041 cgatgacgcc gtcggggttc gcggcgaccg tcccgggcgg gtacgagtac gacgcgtagt
     8101 tcggagcggc cggggtgccg tagaagttgc acgtggcgtc gaccggaccg gttgacgtgg
     8161 gtgccgtcgc ggtgaagaag ccccagttga acgccgcatg gcgaatcgcg taggacgtgt
     8221 tcccgctgaa gttgttcccg gtgatcgtca tgccgttgat acggccaccg ccggcgaccc
     8281 aaccgccgga cccctcggtc gccgaggaga cgcactggcc gccgtcggtg ttgaccgaga
     8341 tgttcacgcc accgttgttg ccggtgaacg tgttgccggt gatggcgttg ttgttggtca
     8401 cgaacttctt gcagagatcg aggtaaaccc ccgacgccag tccggtgaac gtgttccccg
     8461 agatgacgtt gccaccgttg gagatcgtga tccccgccgg gctcgacggc gacgtacggc
     8521 cggttgcgtc gaacgtgttg ccggtaaccg tctggccacc catctgcaca ccggtcgaac
     8581 cggcgagcgc gagggcacgg gacgtgatgc cggtgaaagc gttgccgctc acggtcgcgc
     8641 tggtggccgg ggcacccagc gcctggttga tcgtcaccgg ccgacggaag ttctcgatgg
     8701 tgttgttcga gaaggtgacc gacccgctct gcaccgtgaa ggcggagtcc gtcatcgtcg
     8761 tgcaggtgta cgtgcagttc gacacgaacg acccgcccga gaacacgttg ttctcgacga
     8821 ccgtcgcccc gccgcgcagc cagacgagcg gctgcagcgt tgcgctgatc agtgcggtgt
     8881 caccctgggg gtcgacacgg aacccgtcga tggtcgtcgt gtaggacgtc gtccccgggt
     8941 ttccggggtt gcggaagccc ttcacgatgg actcggcact gcgaacaccg gggctcacac
     9001 cggcagcgat gccggcgttg gcgcccttga acgtcaggtt cttgttcggt gcgacgacct
     9061 cggcgtaggt gccggcggcg acatacacgg tgttcccgga aacggcctga cccacggcgt
     9121 agttcacggt cagacacggg gccgcgctgg ccgaacagtc accggcatcg gtgcccgtcg
     9181 tggcgacgta gcggccgttc ggatcgtccg gcggcaacgt cgtggtggtc gtcgacgtcg
     9241 tggtcgatgt cgaggtggtc gacgtggtcg tgctggtggt cgttgtgctg gtcgtggtgg
     9301 tggtttcctc cggcgtgccg gtctccggtg ggcacgcggt cgtcacgacc gcgaccacag
     9361 acagcacagc aacaagcagg cccgtaggcc gcgcagttct tcgcattcga tccctccaag
     9421 gacacatcgc gttgcgcgcc cacctcccca gcgagcgccg cacgacgatc atgcgcaggc
     9481 gatggttcaa acgtcaacga tcctgaggaa tcttctggtc gccctccccc ataggctgcg
     9541 ctccgtgccg gtcgcgttgt tcctccacca tgacgccaat tcgtcgtcgg gcctgctcgg
     9601 cgagatcgtg accgagctcg gctatgaggt gcgagaccac cacatctgca ccgagctcgc
     9661 ctccggcgtc gccaccggcc cgctgccgac gatcgaaggc gtcgacctcc tgatcccgct
     9721 cggctcgagg tggtcggtct acgacaccga gaacatcgga tcgtggatcg acgacgaggt
     9781 cgaactcgtc cgcgaagccg accgccgcgg tgtccccgtg atcggcgtct gcttcggcgg
     9841 ccaactcgtt gcgaccgccc tcggcggttc ggtcctgccc gcaccgttcg aggagatcgg
     9901 gtggatcgag atcgagccgg tggccgatgt cgagcacccc atcccgacgg gcccctggtt
     9961 ccagtggcac ctcgacgtgt tcaccccgcc ggccgacgcg accgtgctgg cgacctcgcc
    10021 cgcgggtgcg caggcgttcc gcctgcgcga caacctcgca ctccagttcc accccgaggt
    10081 cgacaccgcc gtcctccagg agtggatgat caccgaccgc gatcaactcc gtgagtgcgg
    10141 cgtcgatccc gacgagctcc tcgagcgcac cgcccgggaa acggcgaaag cccgcacccg
    10201 ggccgaagcc gtggtgcggg ccttcctcga cagcgtggac tacgcggcag ccccgaccgg
    10261 cgcctcactg gacgtcgact gaccggagcg cttcgagaac agcaccgcac cgatgagcac
    10321 cacgaccgcg ccaccggcga tcacgaagcg gatgccgtcg tcgctcaacg acagcacggc
    10381 ggggagcacc agcaacgaca cgaggttcat caccttgatg agcgggttga tggcgggacc
    10441 agcggtgtcc ttgaacggat cgcccacggt gtcaccgatc acgaccgcct tgtggacgtc
    10501 ggaccccttg ccaccgtggt tgccttcctc gatgtacttc ttggcgttgt cccacgcgcc
    10561 gccggcgttg gacaggaagt tcgccatcag ctgaccgacg acgatggcgg cgatcaggta
    10621 gccgccgagc gcgtacggac cgaggccgaa cccaacggcc accggcacga gcacggccaa
    10681 gagcgccggc gtgatgagct cgcggagcgc agcgctcgtg cagatgtcga tgaccggtcc
    10741 gtagtcgggc ttctcggtgt agtccatgat cccgggcttc tcccggaact gacgacgaac
    10801 ctcttccacg accacaccgg ccgtgcgacc cacggcgagg atcgccagcg agctgaagag
    10861 gaacaccacc gcgccaccga tgatggcgcc gatgaacgtc ttcggctcgg cgatgttgat
    10921 gacggtgccc tcaccgatcg tctcgacgaa gctggcgaac agcgccaccg cggcgatgac
    10981 cgccgtgccg atcgcgaacc ccttcgtgac ggccttggtg gtgttcccga ccgtatcgag
    11041 gccatcgagg atctcctcgg tgcggccctc gaagacgccc gacatctcgg cgatgccctg
    11101 agcgttgtcg gcgatcggcc cgaacgtgtc ctcggcgacg acgattgccg tggtcgacag
    11161 catgccgata ccggtcaggg cgatgagata gaggccgaac tgggagttgc cgtcaccgag
    11221 accgagcgcc accccgatgg cgacgcaaat ggcgaccgct gcccacaccg ccgactcgag
    11281 gccggagctg agacccgaca acaggaccgt ggccggaccg gtccgaccgg agtttgcgat
    11341 gtccttcacc ggcttgaact ggctcgacgt gaagtactgg gtgatgcgcg aggccgcctg
    11401 tccgaggacc acgcccgcca ggacggcgag gaacatccgc agggcggggt tggagatctc
    11461 ggatccctcg gggttaccga cgtagccgaa cgcgacgacc gccgtgccgc cgagcgccag
    11521 caccgacgcg atgttgagac cgcggttgat cggcttgagc gcgtcgttct cgctctcgtt
    11581 cgccttcacc atgtagatgg cgatgaccga cgccacgagc ccgagcgcca cgacggcgag
    11641 cgggaagatg aggcccttcg ctgcctcggt gccttcgaga ccgaggccac cggcggagaa
    11701 ggccgacacg ccgaggatga tcgacgccac gagcgtcacg ccgaagctct cgaagaggtc
    11761 ggcagccata cccgcacagt cgccgacgtt gtcaccgacg ttgtcggcga tcgttgcggg
    11821 gttgcggggg tcgtcctcgg ggataccggc ctcgaccttg ccgaccaggt cggcgccgac
    11881 gtcggcagcc ttggtgaaga tgccgc
//
LOCUS       DAOSOA010000068        11903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5329_length_11903_cov_6.748143, whole genome shotgun sequence.
ACCESSION   DAOSOA010000068 DAOSOA010000000
VERSION     DAOSOA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11903
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5329_length_11903_cov_6.748143"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2238
                     /locus_tag="PKY13_06280"
     CDS             <1..>2238
                     /locus_tag="PKY13_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOT79575.1"
                     /translation="RAMLSATARVRRGGQTLDVDAADLVPGDIVLLEAGDRIPADARI
                     LVCIDGEADESSLTGESLAVDKTDVAVDRADAPLGDRSCMVWMNTTVTRGRIEAIVVA
                     TAMETEMGRIADLLTTAENTETPLARQLDGLGKRLAGVAGVAMVTYLGVGLARGEAIS
                     DIIGSAIGLAVAAVPEGLPAVVTVTLALGVHQVAKHGAIVKNLSSVETLGSTSVICSD
                     KTGTLTMNRMSAVEVQLAGDRIEVATSADGTASGDGLDAADRLLEVATLASDAVVTIG
                     DGTDGSEVGDPTEIGVVRLAAQRGVDVPALRDRRRRLAEVPFDSGRKFMATIVPTDDP
                     DRVQIAVKGAVDRLAERCTTIATPAGTVDFDNGWRSRVDAGVDGAARQGRRVLAICTT
                     TVNRTAIEGADQAALVELVNDLELVGLIGLADPPRAEAGEAIALAHGAGIAVKMITGD
                     HPTTATAIAGELGIRGETVTGATLDEWSDDQLTDRVEDIGVFARVSPEHKLRIVKSLQ
                     GRGHVTAMTGDGVNDAPSLKQADVGVAMGITGTEVSKEAADVVLADDQLGSIVSAVGQ
                     GRAIYDNIVTFVRFQVATNVGAIITLVGSQLIGLPKPFAAIQLLWINIIMDGPPAMAL
                     GTDVPKPGVMSRLPRARDAVILNRHRISVLLMHGAIMAALTLGILAWAKGEYGESTGA
                     TMAFTAFVLLQIVNALNVRSEGTIFSPTTFTNRWLWAALGTVTVLQILVVEVAPMRNL
                     FETHDL"
     assembly_gap    2239..2338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2339..3708)
                     /locus_tag="PKY13_06285"
     CDS             complement(<2339..3708)
                     /locus_tag="PKY13_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HOT79576.1"
                     /translation="MTDVIRNFINGELVEAADGATTDLIDPCTGDVFATAPRSSAEDI
                     DRACTAAQAGFEVWRDSTPGERQLALLKIADALEARADEFIAAESRNTGKPIGLTASE
                     ELPPMIDQIRFFAGAARNLEGRSAGEYLAGHTSFIRREPIGVCAQIAPWNYPMMMAVW
                     KWAPAIAAGNAVVLKPSDTTPVTTVMMAEMMAEFLPPGVMNVVCGDRDTGRALVGHEI
                     PQLISITGSVRAGMQVAEQASQTLKRCHLELGGKAPVVVFDDADLEAAAEAIAIGGYF
                     NAGQDCTAATRVLAGPKIYDDFVAALTEQARGTATGQPDDEDVLYGPLNNVNQFDRVS
                     SFFDRVPDHATVQTGGDRFGDRGYFFNPTVVSGLRQDDEMVQDEIFGPVITVQRFTDE
                     DEAVRWANGTPYGLASSVFTQNHGRAMRMSRRLDFGCVWINTHIPLVAEMPHGGFKMS
                     GYGKDLS"
     gene            3846..5216
                     /locus_tag="PKY13_06290"
     CDS             3846..5216
                     /locus_tag="PKY13_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT79577.1"
                     /translation="MTDSAHLDRINANAATIADEQMATLLANTTASKALYERAVRSMP
                     GGVASSFQLGDPYPIYLEKGVGAEVWDVDGTGYYDFHNGFGSIAVGHSNPIVAEAVAA
                     AAYRGMHFAVTVESCVELAEELCRRFNVDQVRFTNSGTESNMTAIRVARAATGRDVIA
                     KIEGSYHGHVDQLMYSCTPGAESMGGRDMPAKTPKSKGMPKGLEDWIRIVPFNDAAAF
                     ERLLEAEGDKIAALIMEPVMMNVGIVLPQPGYLEKIRELCTKYGVVLIFDEVKCGVTI
                     AAGGAQERFGVKPDLASWAKSIGGGAVIGAFGGRGDLMGEINNGAAHQGTFNGNPLSV
                     AASLATLTQVLTPEAYVHFDMLGKRLTDGMNAAIAKYGIPAHTIDLGAKGCVSYRSEP
                     LTNYRDFLDTNTGLYDASYPWMINRGVFMTPGDEEQWTMSVMHTTEHVDRYVDAFVGL
                     CEALAA"
     gene            complement(5283..6077)
                     /locus_tag="PKY13_06295"
     CDS             complement(5283..6077)
                     /locus_tag="PKY13_06295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT79578.1"
                     /translation="MKRRSIGSILHKVMSVGVFVYLFVPLVTIVVFTFNKPTTKFNTT
                     WEGFTWDNWLHPFAQKQFTDSLVVSVKVAVVACVLATVLGSLIALAIGRYRMYGSSAV
                     NLLLVLPLTTPEIVMGTSLFTLFFNFGIERGFWTIVIAHTMFCLSFVALTVKARTRGM
                     DWSLEDAAQDLGSSPIRTFWRVTLPGIMPGVAGAFLLSVALSIDDYIITSFVAGPTIT
                     FPRRVFDSARIALPPQVHVLAAIIMLVAITIIVISSTRRSKNSVEA"
     gene            complement(6088..6987)
                     /locus_tag="PKY13_06300"
     CDS             complement(6088..6987)
                     /locus_tag="PKY13_06300"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT79579.1"
                     /translation="MANDGDDRTLKKAKWAPYLLSFPGMLCIYLFFVVPLVTLTKIAL
                     SIRQERGSSKVDFDWQWGNFSKAFTDFGDQFWRALLYAAIATVICVLLAYPLAYFIAF
                     KTGRWQNLMLGLVMVPFFTSFLLRTIAWQSLFADNGPILGVARSLHVTGILDFLQITT
                     DGKIMNTSSAVIGGLVYNFLPFALLPIYVSLEKIPTNLFDAANDLYSPFGSTFRRVIF
                     PLSVPGVFAGTLLTFIPASGDFINAHLLGDVQKPVIGGVIQGRFLQAGDYPTAAAISL
                     VLMLVITAAVLIYARFLGTDDLV"
     gene            complement(7017..9419)
                     /gene="potA"
                     /locus_tag="PKY13_06305"
     CDS             complement(7017..9419)
                     /gene="potA"
                     /locus_tag="PKY13_06305"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HOT79580.1"
                     /translation="MSETTAEPTVVLQSVRKEFGSYVAVEHADFAIRAGEFFSLLGPS
                     GCGKTTLLKMIAGFEQPSAGAVLLEGVDVSSVPPHKRNVNTVFQQYALFPHLSIVDNV
                     AFGLRSKKVPATESRTRSMEMLDVVKLTEFANRRPNQLSGGQQQRVALARALVNLPAA
                     LLLDEPLAALDLKLREAMQIELKRIQRDVGITFVFVTHDQGEALTMSDRIAVMSRGRV
                     EQIGTPSEIYDRPETIFVAGFIGSANLLPGKVSAPLKVQLAVGPTIDVPLLPSGATGS
                     GEDVTVMLRPERLTPTVDTVAGRSVTGRIDDVIYQGSETLLIVHLEDETEVLVTIDPD
                     HATAATKVGNQVTLTWSPDAPFVMPGRTAIVGATGTDFNEVEATMAGDLDADDDVEGG
                     EGHPNAAPPATPEKLRNEGRRKLLIGSGVAIVAGLGAVGLSNVGSNEGGSSGTSSSSG
                     GAAGGGLGQGDTDLNIINWTEYIDPTADGESGTIDLFSKEFNVKTNYSETWNDNNASY
                     GTEFVAYLEGGNSTPWDIAMPTYWMAARIKSKDWLAPLPFNLIPNYVNLDEQYLNTEW
                     DPGSKFNLPWQAGVTGIAYNIAETGRELTSVADLFAPDLKGRVGFFTEMRDTLGLVML
                     EMGNDPRKPTEDTINAALDRIEQAKNDGQIRRFTGNDYLQDIQNGNFAACIAWSGDIA
                     QASNPDVKFVFPEEGAMNWFDTMVIPIGAPNGVAAAEFMNYVYDPVNAAKITSYVQYI
                     SPVKGVREELEKLGGDAAALAENPLLFPGDEEKARMYIFGDMPEALDESVTDRFTTMT
                     GA"
     gene            9595..10947
                     /locus_tag="PKY13_06310"
     CDS             9595..10947
                     /locus_tag="PKY13_06310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT79581.1"
                     /translation="MTSLWNATIPDLDHPRPALSLHTGDADAWDVVIVGGGFTGLWTA
                     RSLAELDPGLRILVVEAERCGFGASGRNGGWCSALFPTAYDTLADRHGAAAADAQRQA
                     MRAAVDEVGAAAAADGIDCHFAKGGTLTLSTSPAQTDRVRAHAEPDDVWLDADAARVR
                     VNATGVLGAMYTPHCAALHPGRLAVGLAAAVEQRGVRIVENTRIGAIAPGLVTCIDGQ
                     SIRAGAVIRATEGYTPTLRGHRRTVAPLYSLMIATEPLPDESWASIGLTERETFTDGR
                     HMIIYGQRTADGRFAFGGRGAPYHFGSRTRPAFDLDDRVFGLLEATLRSMFPQIGSAA
                     VTHRWGGPLGVPRDWMASVGFDPHSRLGWAGGYVGDGVSTTNLAGRTLADLVLGRETP
                     LTALPWVGHRSPRWEPEPARWLGINGGRALADVADRSEGRTGRPARWAERLMAKLSGG
                     "
     gene            complement(11016..11462)
                     /locus_tag="PKY13_06315"
     CDS             complement(11016..11462)
                     /locus_tag="PKY13_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HOT79582.1"
                     /translation="MTDITNDENYEPSPWEPVAEQVERYLATDGAEGHEWMGGHCIIL
                     TTTGRRTGKLRRTPLMKVKHGDGYIVIASLGGAPQHPQWYLNITEHPDVVINDLAERH
                     ELVARTASPEEKRELWPLAVAEWPDYDKYQESTDRDIPLVICEPKA"
     gene            11574..>11903
                     /locus_tag="PKY13_06320"
     CDS             11574..>11903
                     /locus_tag="PKY13_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79583.1"
                     /translation="MTQHRRLATLAALLLLATTLVACQNGKVGGRCKAGTVAQDATHV
                     LVCKKGKWSRLISKADGMRELARIQAEEAARAQPPATQPPATEPPATAPPATVPPTTA
                     PVVTTPPT"
BASE COUNT         1932 a         4003 c         3968 g         1900 t
ORIGIN      
        1 gcgcgccatg ctcagcgcca cggcccgggt gcgacggggt ggccagaccc tcgacgtcga
       61 cgccgccgac ctcgtccccg gtgacatcgt cctcctcgag gccggcgacc gcatccccgc
      121 cgacgcccgc atcctggtct gcatcgacgg cgaggccgac gagtcgtcgc tgaccggcga
      181 atcgctggcg gtcgacaaga ccgacgtggc cgtcgaccgg gccgatgccc cgctcggcga
      241 tcgctcgtgc atggtctgga tgaacacgac cgtgacccgc ggccgcatcg aggccatcgt
      301 cgtcgccacc gcgatggaga ccgagatggg ccgcatcgcc gacctgctca ccaccgccga
      361 gaacaccgaa accccgctcg cccgccagct cgacggcctc ggcaaacgcc tcgccggcgt
      421 ggccggcgtc gcgatggtca cctacctcgg cgtgggcctc gcccgcggtg aggcgatcag
      481 cgacatcatc ggttccgcca tcggcctcgc cgtcgccgcc gtgcccgaag gtctcccggc
      541 cgtcgtcacg gtgacgctcg cactcggtgt gcatcaggtc gcgaaacacg gggcgatcgt
      601 caagaacctc tcgagcgtcg agacgctcgg atcgacatcg gtgatctgct ccgacaagac
      661 cggaaccctc accatgaacc gcatgtcggc ggtcgaggtg cagttggccg gcgaccgcat
      721 cgaggtcgcc acatctgcgg acggcactgc atcgggcgac ggcctcgacg ccgccgaccg
      781 cctcctcgag gtcgcgacgc tcgcgtcgga cgccgtcgtg accatcggcg acggaaccga
      841 cggctccgag gtcggagatc ccaccgagat cggcgtggtg cgcctggcgg cacagcgcgg
      901 cgtcgacgtt cccgcactcc gggaccgtcg gcgccgcctc gccgaagtgc cgttcgactc
      961 cggccgcaag ttcatggcca ccatcgtccc gaccgacgac cccgaccggg tccagatcgc
     1021 cgtgaagggc gcggtcgacc ggctcgccga gcgctgcacc acgatcgcca caccggcggg
     1081 aacggtcgac ttcgacaacg ggtggcgcag ccgggtcgac gccggtgtcg acggtgccgc
     1141 ccgccagggc cgccgagtgc tggcgatctg caccacgacg gtgaaccgca ccgcgatcga
     1201 gggcgccgac caggccgcgc tcgtcgagct cgtcaacgat ctcgaactcg ttgggctcat
     1261 cggtctggcc gacccgcccc gggccgaggc cggcgaggcc atcgccctcg cccacggcgc
     1321 cggcatcgcc gtgaagatga tcaccggcga ccaccccacg accgccaccg cgatcgccgg
     1381 cgagctcggc atccgcggtg agaccgtcac cggagccacg ctcgacgagt ggagcgacga
     1441 ccagctgacc gatcgggtcg aggacatcgg cgtgttcgcc cgggtgtcgc ccgagcacaa
     1501 gctgcgcatc gtgaagtcgc tgcagggccg cgggcacgta acggccatga ccggcgacgg
     1561 tgtcaacgac gcaccgtcgc tcaagcaggc cgacgtcggc gtggcgatgg gcatcaccgg
     1621 caccgaggtg tcgaaggaag cggccgacgt cgtccttgcc gacgaccagc tgggcagcat
     1681 cgtgtccgcc gtcggacagg gccgcgccat ctacgacaac atcgtgacct tcgtccggtt
     1741 ccaggtcgcc accaacgtcg gcgccatcat cacgctggtc ggctcccagc tcatcgggct
     1801 gccgaagccg ttcgcagcga tccagctgct ctggatcaac atcatcatgg acggtccgcc
     1861 ggccatggcc ctcgggaccg acgtcccgaa gcccggcgtg atgtcacgcc tgccccgggc
     1921 ccgcgacgcc gtcatcctca accgccaccg gatctcggtg ctgttgatgc acggcgccat
     1981 catggcggcc ctcacgctcg gcatcctcgc ctgggcgaaa ggtgagtacg gcgagtccac
     2041 cggcgcgacg atggccttca cggccttcgt cctgttgcag atcgtcaacg ccctcaacgt
     2101 ccggtccgag ggcacgatct tcagcccgac gaccttcacc aaccgctggt tgtgggcggc
     2161 gctcggcacc gtgaccgtcc tgcagatcct cgtggtcgag gtcgccccca tgcgcaacct
     2221 cttcgagacc cacgacctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     2341 gaggtccttg ccgtagcccg acatcttgaa cccgccgtgg ggcatctcgg ccacgagggg
     2401 gatgtgggtg ttgatccaca cgcagccgaa gtcgaggcgg cgggacatac gcatcgcccg
     2461 cccgtggttc tgggtgaaga cggagctggc gaggccgtac ggcgtgccgt tggcccagcg
     2521 caccgcttcg tcctcgtcgg tgaaccgctg cacggtgatg accgggccga agatctcgtc
     2581 ctgcaccatc tcgtcgtcct ggcggagacc cgagacgacg gtggggttga agaagtagcc
     2641 gcggtcgccg aagcggtcgc cgccggtctg caccgtggcg tgatcgggga cccggtcgaa
     2701 gaacgacgac acccggtcga actggttgac gttgttgagc gggccgtaga gaacgtcctc
     2761 gtcgtcgggc tgtcccgtgg ccgtgccgcg cgcctgctcg gtgagcgccg ccacgaagtc
     2821 gtcgtagatc ttcgggccgg cgagcacccg cgttgcggcg gtgcagtcct ggccggcgtt
     2881 gaagtacccg ccgatcgcga tggcctcggc ggccgcctcg aggtcggcgt cgtcgaagac
     2941 cacgaccggt gccttgccgc cgagctcgag gtgacagcgc ttcagcgtct gtgacgcctg
     3001 ctcggccacc tgcatgcccg cacgaaccga gcccgtgatc gagatcagct gggggatctc
     3061 gtgaccgacg agcgctcgac cggtgtctcg gtcgccacac accacgttca tgacgccggg
     3121 cgggaggaac tccgccatca tctcggccat catcaccgtg gtcaccgggg tggtgtcgga
     3181 cggcttgagc accacggcgt tgccggcagc gatggcgggc gcccacttcc acaccgccat
     3241 catcatcggg tagttccacg gcgcgatctg ggcgcacacg ccgatcggct ctcgacggat
     3301 gaacgacgtg tggccggcga ggtactcgcc cgccgatcgg ccctcgaggt tgcgggccgc
     3361 accggcgaag aaccggatct ggtcgatcat cggaggaagt tcttcggacg ccgtgagccc
     3421 gatcggcttg ccggtgttgc gcgactcggc ggcgatgaac tcgtcggccc gggcttcgag
     3481 cgcgtcggcg atcttcagga gggcgagctg ccgttcgccc ggtgtcgagt cccgccacac
     3541 ctcgaagccg gcctgcgctg cggtacaggc gcggtcgatg tcctcggcgc tcgagcgagg
     3601 cgccgtggcg aacacgtcgc cggtgcaggg atcgatcagg tcggtcgtgg cgccgtctgc
     3661 ggcctcgacg agctcaccgt tgatgaagtt gcgaatcacg tcggtcatgg aggtcgagcc
     3721 ttgctcagac cgttgcgatt tgcaatggtt cctgcgaatt cactacctcg aagacgcctg
     3781 gaattgcgaa taccgtcgta gtagcctgac gttccaacgg atttcctcga aaccaggtgc
     3841 ctcccatgac cgatagcgct cacctcgacc ggatcaacgc caacgcggcg accatcgccg
     3901 acgagcagat ggccacgctc ttggccaaca ccacggcctc caaggctctc tacgagcggg
     3961 cagtccgttc gatgcccgga ggcgtcgcct cgagcttcca gctcggcgac ccctacccca
     4021 tctatctcga gaagggcgtc ggcgccgagg tgtgggacgt cgacggcacc ggctactacg
     4081 acttccacaa cggattcgga tcgatcgccg tcgggcactc caacccgatc gtcgccgaag
     4141 ccgtcgccgc tgcggcctac cgaggcatgc acttcgccgt caccgtcgag agctgcgtcg
     4201 aattggccga ggagctgtgc cgccgcttca acgtcgacca ggtgcgcttc accaactcgg
     4261 gaaccgagtc gaacatgacc gccatccggg tggctcgcgc cgcgacgggc cgcgacgtca
     4321 tcgccaagat cgagggcagc taccacggtc acgtcgacca gctgatgtac agctgcacgc
     4381 ccggcgccga gtcgatgggc ggccgcgaca tgccggcgaa gacccccaag tccaagggca
     4441 tgcccaaggg actcgaggac tggatccgca tcgtgccgtt caacgacgcc gcagcgttcg
     4501 aacgcctgct cgaggccgag ggcgacaaga tcgccgcact catcatggag ccggtgatga
     4561 tgaacgtcgg catcgtgctc ccccagcccg gctacctcga gaagatccgc gagctgtgca
     4621 ccaagtacgg cgtcgtcctc atcttcgacg aggtcaagtg cggtgtgacg atcgctgccg
     4681 gcggcgcgca ggagcgcttc ggggtcaagc ccgatctcgc cagctgggcc aagtcgatcg
     4741 gcggcggtgc cgtcatcggg gcgttcggcg gtcgcggcga cctcatgggc gagatcaaca
     4801 acggtgcagc ccaccagggc accttcaacg ggaacccgct gtcggtcgcg gcgtcgctcg
     4861 ccacgctcac ccaggtcctc acgcccgagg cctacgtgca cttcgacatg ttgggcaagc
     4921 gactcaccga cggcatgaac gcagcgatcg cgaagtacgg catcccggcg cacacgatcg
     4981 acctcggcgc caagggctgc gtgtcctacc gttccgagcc gctcaccaac taccgcgact
     5041 tcctcgacac caacaccggc ctctacgacg cctcctaccc gtggatgatc aaccgcggtg
     5101 tgttcatgac gcccggcgac gaggaacagt ggaccatgtc ggtcatgcac acgaccgaac
     5161 acgtcgaccg ctacgtcgac gccttcgtcg gtctgtgcga ggccctcgcc gcgtagtccc
     5221 cgccaccggt cggtcctcga ccggtggttc ctcgacgact cgggcctccg ggcctcgacg
     5281 actcaggcct cgaccgagtt cttcgagcgg cgtgtgctcg agatcacgat gatcgtgatc
     5341 gccacgagca tgatgatcgc cgccagtacg tgcacctgcg gcggcagcgc gatgcgcgcc
     5401 gagtcgaaga cccgccgggg gaacgtgatg gtcgggccgg ccacgaacga cgtgatgatg
     5461 tagtcgtcga tcgacaacgc gaccgacaac aggaacgcac cggccacacc gggcatgatg
     5521 ccgggaagcg tcactctcca gaaggtccgg atcggggacg aacccaggtc ctgcgccgcg
     5581 tcctcgagcg accagtccat gccgcgcgtc cgggccttca ccgtcagcgc cacgaagctg
     5641 aggcagaaca tcgtgtgcgc gatcacgatc gtccagaagc cgcgctcgat gccgaaattg
     5701 aagaacagcg tgaagagcga cgtgcccatc acgatctcgg gcgtggtgag cggcaggacc
     5761 aggaggaggt tgaccgccga actgccgtac atgcggtacc gcccgatcgc cagcgcgatg
     5821 agactgccga gcacggtggc cagcacacac gcgaccacgg ccaccttcac gctgacgacg
     5881 agggagtccg tgaactgctt ctgggcaaag gggtggagcc agttgtccca cgtgaagccc
     5941 tcccacgtgg tgttgaactt ggtggtgggc ttgttgaacg tgaacaccac gatcgtgacg
     6001 aggggcacga acaggtacac gaacacgccg accgacatga ccttgtggag gatcgacccg
     6061 atgctgcgac gtttcatggt tgcctcctca caccaggtcg tcggtcccga ggaaccgggc
     6121 gtagatcaac actgcggcgg tgatcaccaa catcagcacc aggctgatgg ccgcggcggt
     6181 cggatagtcg cccgcctgca ggaaccgccc ctggatcacg ccgccgatca ccggcttctg
     6241 gacatcgccc aacaggtggg cgttgatgaa gtcgccggat gccgggatga aggtgagcaa
     6301 ggtgccggcg aagactccgg gcaccgacag cgggaagatc acccgccgga acgtgctccc
     6361 gaacggcgag tacagatcgt tcgcggcgtc gaagaggttc gtcgggatct tctcgagact
     6421 cacgtagatg ggcagcaacg cgaacggcag gaagttgtag acgagcccgc cgatgaccgc
     6481 ggacgacgtg ttcatgatct tgccgtcggt ggtgatctgc aggaagtcca agatgccggt
     6541 gacatgtagg gagcgggcga cgcccagtat cggaccgttg tcggcgaaga gcgactgcca
     6601 ggcgatggtg cgcaggagga agctcgtgaa gaacggcacc atgaccaggc ccagcatcag
     6661 gttctgccaa cgaccggtct tgaaggcgat gaagtacgcc agcgggtacg ccagcaggac
     6721 gcagatcacg gtcgcgatcg cagcgtagag cagcgcccgc cagaactggt caccgaagtc
     6781 ggtgaacgcc ttcgagaagt tgccccactg ccagtcgaag tcgaccttgc tcgacccccg
     6841 ttcctgtcgg atcgacaagg cgatcttcgt gagggtcacg agcggcacca cgaagaacag
     6901 atagatgcag agcatcccgg ggaacgacag gaggtagggc gcccatttgg ctttcttgag
     6961 ggttctgtcg tcgccgtcgt tcgccatgtg gttccttcct cgatcgtggg agcggctcag
     7021 gcgccggtca tggtggtgaa gcggtccgtg accgactcgt cgagggcctc gggcatgtca
     7081 ccgaagatgt acatgcgggc cttctcctcg tcacccggga acaggagcgg gttctcggcg
     7141 agggcggcgg cgtcaccacc caacttctcg agctcctccc tcaccccctt gaccggggag
     7201 atgtactgca cgtaggaggt gatcttcgcg gcgttgaccg ggtcgtacac gtagttcatg
     7261 aactccgccg cagcgacacc gttgggagcg ccgatcggga tcaccatcgt gtcgaaccag
     7321 ttcatcgcac cctcttcggg gaacacgaac ttcacgtcgg ggttcgaggc ctgcgcgatg
     7381 tcgcctgacc acgcgatgca cgccgcgaag ttgccgttct ggatgtcctg caggtagtcg
     7441 ttgccggtga acctgcggat ctggccgtcg ttcttggcct gctcgatgcg atcgagcgcc
     7501 gcgttgatgg tgtcctcggt gggcttgcgt ggatcgttgc ccatctcgag catcaccagg
     7561 ccgagggtgt cgcgcatctc ggtgaagaac ccgacccggc ccttgaggtc gggggcgaag
     7621 agatcggcga cactcgtgag ttcgcgccct gtctcggcga tgttgtaggc gatgcccgtc
     7681 acgccggcct gccacggcag gttgaacttg ctccccggat cccattcggt gttcaggtac
     7741 tgctcgtcga ggttgacgta attggggatc aggttgaacg gcagcggcgc gagccagtcc
     7801 ttgctcttga tgcgggcggc catccagtag gtgggcatcg cgatgtccca cggcgtggag
     7861 ttcccgccct cgagataggc gacgaactcg gtgccgtagc tggcgttgtt gtcgttccag
     7921 gtctcggagt agttcgtctt gacgttgaac tccttggaga acagatcgat cgtgccggac
     7981 tcgccatcgg ccgtcgggtc gatgtactcg gtccagttga tgatgttgag gtcggtgtcg
     8041 ccctgcccca gcccgcctcc tgcggctcca ccgctcgagc tggacgtccc gctcgagccg
     8101 ccctcgttgc tgcccacgtt ggacagcccg acggcgccga gaccggcgac gatcgccaca
     8161 ccgctcccga tcaacagctt gcggcgccct tcgttgcgca gcttctcggg cgtggcgggc
     8221 ggagcggcgt tcgggtggcc ctcgccgccc tcgacgtcgt cgtcggcgtc gagatcgccg
     8281 gccatcgtgg cctcgacctc gttgaagtcg gtaccggtgg cgcccacgat ggcggtgcgc
     8341 cccggcatga cgaacggagc atcgggcgac cacgtgagcg tgacttggtt gccgaccttc
     8401 gtggcggccg tggcgtggtc gggatcgatg gtgaccagca cctcggtctc gtcctcgagg
     8461 tggacgatga ggagcgtctc ggaaccctgg tagatgacgt cgtcgatgcg acccgtgacc
     8521 gagcggcccg ccacggtgtc cacggtgggc gtgagccgct cgggacgcag catgacggtg
     8581 acgtcctcac cgctgccggt cgccccggag gggagcaacg ggacgtcgat ggtcgggccg
     8641 accgccaact ggaccttgag gggggcgctg accttgcccg gcaggaggtt ggcggaaccg
     8701 atgaagccgg cgacgaagat cgtctcgggt cggtcgtaga tctccgaagg ggtgccgatc
     8761 tgctcgacgc gaccacggct catgacggcg atgcgatcgc tcatcgtgag cgcctcacct
     8821 tgatcgtggg tgacgaacac gaaggtgatg ccgacgtcgc gctggatccg cttcagctcg
     8881 atctgcatcg cctcgcggag cttgaggtcg agcgcggcga ggggctcgtc gagcaacagt
     8941 gccgccggca ggttgacgag cgctcgggcc agggcgaccc gctgctgctg gccgcccgac
     9001 agctggttgg ggcgccggtt cgcgaactcg gtgagcttga cgacgtcgag catctccatc
     9061 gaccgcgtgc gcgactcggt cgccggcacc ttcttcgagc ggaggccgaa ggcgacgttg
     9121 tcgacgatcg agaggtgcgg gaacagcgcg tactgctgga acaccgtgtt gacgttgcgc
     9181 ttgtgcggcg gcaccgacga gacgtcgacg ccttcgagca ggacggcacc ggcagacggc
     9241 tgctcgaagc cggcgatcat cttgaggagg gtcgtcttgc cacaaccgga ggggccgagc
     9301 agtgagaaga actcgccggc cctgatcgcg aagtctgcgt gttcgacggc gacgtacgaa
     9361 ccgaactcct ttcggacgct ctgcaggacg acggttggct cggcggttgt ctcgctcatc
     9421 gacacccttc cacgatgatc ttcttgccgc gtggccggac ctgagcgacg cgtggccgaa
     9481 acatagcgcc gccatcgcgg ggatgcctcg gattcgtgtt ccattttccg ggacgagatg
     9541 agatgttcac agaggtttct cgcagcaaag gcgacatcta ggctcgaatt gcctatgaca
     9601 tcgctctgga acgcgacgat tcccgacctc gatcacccgc ggcccgcgct cagtcttcac
     9661 acgggcgacg ccgacgcgtg ggacgtcgtg atcgtgggcg gcggcttcac gggactgtgg
     9721 acagcccggt cgctggcgga gctcgacccc ggcctgcgca tcctcgtcgt cgaggccgaa
     9781 cggtgcgggt tcggcgcgtc ggggcgcaac ggcggctggt gctcggcgtt gttccccacg
     9841 gcctacgaca cgctcgccga ccggcacggt gcggcggcgg ccgacgcaca gcgccaggca
     9901 atgcgcgccg cggtcgacga ggtcggtgcc gctgcggccg ccgacggcat cgactgccac
     9961 ttcgccaagg gcgggacgtt gacgttgtcg acgagccccg cccagaccga ccgggtgcgg
    10021 gcgcatgccg agcccgacga cgtgtggctc gacgccgatg cggcgagagt tcgagtgaac
    10081 gccacgggtg tgctcggcgc gatgtacacc ccgcactgcg cagcactcca ccccggccgg
    10141 ctggccgtcg ggctcgccgc tgccgtcgag caacgcggcg tccgcatcgt cgagaacacc
    10201 cggatcggcg cgatcgcacc cggactcgtg acgtgcatcg atgggcagtc gatccgcgcc
    10261 ggtgccgtca tccgcgcgac cgaggggtac acaccgacgc tgcggggaca ccggcgaacg
    10321 gtcgcaccgc tctactcgct gatgatcgcg accgaaccgc tgcccgacga gtcgtgggcg
    10381 tcgatcgggc tcaccgaacg ggagaccttc accgacggcc gccacatgat catctacggc
    10441 cagcgaaccg ccgacggtcg gttcgcattc gggggccggg gcgcgcccta ccacttcggg
    10501 tcgaggactc gtccggcgtt cgacctcgac gaccgggtct tcgggctgct cgaggcgacg
    10561 ctgcgctcga tgttccccca gatcggcagc gctgcggtga cgcatcgatg gggcggaccg
    10621 ctcggcgttc cgcgcgactg gatggcgtcg gtcgggttcg atccgcactc gcggctcggc
    10681 tgggccgggg gctacgtcgg cgacggggtc tcgacgacga acctcgccgg gcggacgctc
    10741 gccgacctcg tgctcggccg cgaaacgccg ctcactgcac tgccgtgggt cgggcatcgt
    10801 tcaccacgct gggaacccga accggcccga tggctcggca tcaacggcgg gcgcgccctc
    10861 gccgatgtcg ccgaccgttc ggagggtcgc accggccgcc cggcccggtg ggccgaacgg
    10921 ctgatggcga agctctctgg aggctgaccc cgctcgttct gtcccgcgaa aggtgcaggc
    10981 tgcacgcttt cgcgggacga accgggcggg gcgggtcagg ccttgggctc gcagatgacg
    11041 agcgggatgt cgcggtcggt gctctcctgg tacttgtcgt aatcgggcca ctcggcgacg
    11101 gcgagcggcc agagctcgcg cttctcctcg ggcgacgccg tccgggcgac gagctcgtga
    11161 cgctccgcca ggtcgttgat gacgacgtca gggtgctcgg tgatgttgag gtaccactgc
    11221 ggatgctgcg gggcaccgcc cagcgaggcg atcacgatgt agccgtcgcc gtgcttcacc
    11281 ttcatgagcg gcgtgcggcg gagcttgccg gtgcggcgac ccgtcgtcgt gaggatgatg
    11341 cagtggccgc ccatccactc gtgcccttcg gcgccgtcgg tcgcgagata tcgctcgacc
    11401 tgctcggcga ccggctccca cgggctcggt tcgtagttct cgtcgttggt gatgtcggtc
    11461 atcggcgaaa tctaccgccg cccgggaacg ggggatgacg tcgatgtcga gcttcccttc
    11521 ggcgctgaac atcgctacgt tcacgcagtt cgaatttctc gaaatggatt gaaatgacac
    11581 aacatcgccg tcttgccacc ctcgctgccc ttctcctcct cgccaccacc ctcgttgcct
    11641 gccagaacgg caaggtcggc gggaggtgca aggccgggac ggtcgcgcag gacgccaccc
    11701 acgtgctcgt gtgcaagaag ggcaagtgga gccgcctgat ctccaaggcc gacggcatgc
    11761 gcgagctcgc ccgcatccag gccgaggaag ccgcccgggc ccagccgccg gcaacgcagc
    11821 cgccagcgac tgagccgccg gccaccgctc ccccggcgac cgtcccaccc accaccgctc
    11881 cggtcgtgac cacgccgccg acc
//
LOCUS       DAOSOA010000069        11843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5363_length_11843_cov_8.182728, whole genome shotgun sequence.
ACCESSION   DAOSOA010000069 DAOSOA010000000
VERSION     DAOSOA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11843
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5363_length_11843_cov_8.182728"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKY13_06325"
     CDS             complement(<1..394)
                     /locus_tag="PKY13_06325"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOT79584.1"
                     /translation="MARILVIDNYDSFVYNLVQYLGELGAEPMIWRHDALDLDDVERI
                     APDGILISPGPGTPDDAGLSNVLIAEWSARVPVFGVCLGMQCMGQVFGGDVVRAPEIV
                     HGKTSLVRHSGAGVFAGLPNPLEATRYHS"
     gene            535..846
                     /locus_tag="PKY13_06330"
     CDS             535..846
                     /locus_tag="PKY13_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HOT79585.1"
                     /translation="MKRRIEGGADAQTSTGRTTPKGTRPAGEGPAASTRYTPPTPATK
                     YDMPSPMWVPVLMFSFFGIGLLAIFLNYTEVLPGAPTQWYLLGGLMSILFGIVTATQL
                     R"
     gene            complement(1002..1328)
                     /locus_tag="PKY13_06335"
     CDS             complement(1002..1328)
                     /locus_tag="PKY13_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79586.1"
                     /translation="MRRVSLVFWLVGVVIAIAVATLAFDTSIVQTTIAVLIGAVLIKI
                     GLAMITGLAQPVPEPLEAGKLRKVKLVYRCSICGAEVKMTVASSEDPEPPRHCLEDME
                     LVTPIE"
     gene            complement(1375..2577)
                     /locus_tag="PKY13_06340"
     CDS             complement(1375..2577)
                     /locus_tag="PKY13_06340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT79587.1"
                     /translation="MTTAVIVDAIRTGGGKRNGDLSGWHPADLAAEVLKALVERNNLD
                     PALVDDVIMGCVMQVGGQALNVGRNAVLAAGFPESVPATTIDRQCGSSQQSAHFAAQG
                     VMAGAYDIAIAAGVEVMSLVPMGASVGDIKRTGFPFGDQVGLRYADQGGLVPQGISAE
                     MIADQWDISREELDAFGAESQRRAAQAQAEGRFDNEIVAVQKKRRDKETGNVVVLDEL
                     HTSDEGIRPDTSAESLANLKPAFKPDGKVTAGNSSQICDGASAALIMSEEKAKELGLT
                     PRARFHTFALAGVDPVTMLTGPIPSTQKAIARSGGLTLDDIDLFEVNEAFASVVLAWA
                     KETGADLAKTNVNGGAIALGHPLGGSGTKLMATLLNELERTGGRYGLQTMCEGGGLAN
                     ATIIERLG"
     gene            2720..4528
                     /locus_tag="PKY13_06345"
     CDS             2720..4528
                     /locus_tag="PKY13_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HOT79588.1"
                     /translation="MATSTDEQLRDQVKGQTVVTEFLKTVDAHGSDRALRWMDGEDWK
                     ALTFDELADQVARVAGGLAELGIGRGDRVVLMLRNVHQFHVADLAVLLCGATPVSIYN
                     SSSPDQIAYLAGHCEAKLGIIEDAGFLARFTEVADQLPDLTTLVLVNPSPDVDVEVVE
                     WSSLLEHEPVDLAEAAKVAQPEDLATIIYTSGTTGNPKGVMLDHFNLCWTLACLREAF
                     ETETFAGQKVVSYLPMAHIAERATSHYGLAMNGYEVSCCPDPSLIPTYAREVKPNIMF
                     GVPRVWEKIYSGVTAALSADEEKKRQVDEAVAAAIPIVEKITWGTATDEEKETYAFLD
                     AVAFSTIRQLIGLDELDLAVTGAAPITPELLQWFRAIGVPLAEIYGMSESTGPISFAA
                     RKPKAGTVGPAVPGLTLAIADDGEVIFRGGNVFKGYLNDPEKTAEAIDEDGWVHTGDI
                     GEIDDDGYLRIVDRKKELIITAGGKNISPANLEAALKTIPLVGQAAAIGDNRPFVAAL
                     VVLDPEAAPVWAAQHGLEGKSLDELAEEPAVIDEINAGLADAMSRFNNAERVKRVKVL
                     GEEWLPDSDLMTPTSKLKRRGIHAKFAEEIEAFYGG"
     gene            4554..5789
                     /locus_tag="PKY13_06350"
     CDS             4554..5789
                     /locus_tag="PKY13_06350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT79589.1"
                     /translation="MLVSRFPKVTETFQLREMVALEKMGVPIELYAITHHDDGGTVQA
                     EAAALDARANYFPRMSWEILKAQFIWLRRNPAGYVKAWKWSIASNLGAPEFLLRSFLL
                     VPLAAAMALRMERDGVEHVHAHFATYPTHAALVIKWLAGIPFSFTGHAHDIQVRQDGI
                     GAKIEESEFFVCCTRYSLDSLRSLYGDIVDEKGNVVYHGVDLDQFTFREPHSDDGERA
                     FRMVCVATFEEFKGHTYLIDAVRLLAERGVAVELVLIGGDPPRRSTLQDDIRAQVTAA
                     GLDDSVRFLGKVPSAEVRTWIEWSDIGVLACCRQPDGQMDGLPNFLTECEAMGRPVVS
                     TRQAPVMELIVDGENGKLARTRDAVELADAIESMRRLDPEARAKMGLAGHERVQREHD
                     VTVNTAALYDIYLDRVGRN"
     gene            complement(5786..6445)
                     /locus_tag="PKY13_06355"
     CDS             complement(5786..6445)
                     /locus_tag="PKY13_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT79590.1"
                     /translation="MLPDEVAHLAVTTQLPDGAVLSLRPIRPDDGPMLAEGFRRLSPE
                     SRFLRFFSPLDHLSAAQLRYFTDIDYVDHFAWVATIATPDDPALGVGVARYIRSKSDP
                     SAAEAAVAVADDHHRRGIGSIMLEALAAVALTRGITAFHLLVRSDNHAMIAAMEDLKA
                     GRRPNDDASVIRFVLDLPDLADEMRGTAMWKVFRYVGEGSASIQSSVGQRWLGDPPIS
                     T"
     gene            complement(6460..6921)
                     /locus_tag="PKY13_06360"
     CDS             complement(6460..6921)
                     /locus_tag="PKY13_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79591.1"
                     /translation="MTVHPPVPTSTQLGVSNTKMLWRGFLRRCPACGGRKTHSSYATL
                     LNECPQCSLKFERIFGHSIGYIGLNTVVTFAVTFVVLLGGSIATQPDIPVVPLLIATL
                     SAAGLLPILFLPSAHTMWTAIDLAVRPLTAGEIDPRFIKVDPVVGSWSGRR"
     gene            7056..7916
                     /locus_tag="PKY13_06365"
     CDS             7056..7916
                     /locus_tag="PKY13_06365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT79592.1"
                     /translation="MDQRTIVITGANTGIGLETAVSLASAGERVIIACRNPAKADAAV
                     AEISARAASSDVSAVPLDLASLASVRACAAELGRRCERIDVLINNAGLIQSGRETTVD
                     GFETTFGVNHLGHFLLTDLLGDLVKAAPTPRVINLASVAHWFAVGGLNFEDLQSVRFY
                     NVWVAYGRSKLANILFTDELARRWRDAGVCVSSVHPGVVRSGFGQDGDTRGIADKLID
                     ISKPFSITPDRGADTSVWLATAAAGADLDRSGTFWASRKPSLRAPWARRRTDAARLWA
                     ESEELIASVS"
     gene            complement(7926..8408)
                     /locus_tag="PKY13_06370"
     CDS             complement(7926..8408)
                     /locus_tag="PKY13_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT79593.1"
                     /translation="MEAIVDGDREELDYGTFGVATRQLCQQVVDSGFEPDWIVAIARG
                     GLLIGGAVAYALRCKNIGVVNVEFYTDIDERLEVPVMLPPALNLADLADTKLLIVDDV
                     ADTGETLKLVVDTCAPVVAEIRSLVIYEKPRSIFSADYVWRHTDQWINFPWSSEPPLR
                     "
     gene            complement(8418..8900)
                     /locus_tag="PKY13_06375"
     CDS             complement(8418..8900)
                     /locus_tag="PKY13_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79594.1"
                     /translation="MAPNARDERIDTNDPQRHLVYAAEDSVLDDIGRRLRRWTDVEAF
                     VEAILADPAYLDLFPDAPLDVVLDRRSRSARASVALPDRATILIRDGSWNALTVLHEL
                     SHLVSPDREPHGADFVATELALVRRFCGFDAFATLASTFVAHGVAAASVPLASARGAD
                     "
     gene            complement(8914..9678)
                     /locus_tag="PKY13_06380"
     CDS             complement(8914..9678)
                     /locus_tag="PKY13_06380"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HOT79595.1"
                     /translation="MPPPTDTDGRLATIRALLAKAEATTFAAEAEAFTAKASELMARY
                     AVDEAMLWAGARDTSGTPTEIRLVLHRPFTAQKAVLVNAVANAYGCQALRLGRADDGA
                     TEHMSVIGFASDLSLVETLVTSLFLQLTSAMTAPLPGPAGRPASQVAAWRRSFIMGFT
                     ATITERLRADRARATAETDRERHPEAGEQSAAVVLRDRSAAVDDEFRQRYPRIRTSRV
                     SSGTSESGRRAGTSAGRTADLGARRIGHRQALGQGN"
     gene            10012..10983
                     /locus_tag="PKY13_06385"
     CDS             10012..10983
                     /locus_tag="PKY13_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT79596.1"
                     /translation="MSAIIEARGLTKSFGDTHALAGLDLTAEAGRVTALLGPNGAGKT
                     TFISAVSTLLRPDSGELRVAGIDVLAEPKKVRQVIGLAGQYASVEPAMTGIENLDMVA
                     RLFGLDRVNAKRAAGEVLERIGLTEAGTRVVRDYSGGMRRRLDLGASLVGRPRLLLLD
                     EPTTGLDPRSRSELWDAIRDLVSDGTDVLLTTQYLEEADQLARDVVIVDHGRVIASGT
                     PDELKDQAGRDVIEVRPRSAGDLAAVAEILASVAAEEPRSDDDSHRITATVDGGAGDL
                     ATVVRLLDERGLQVDDIGLRRPTLDEVFLTLTGQPIDPSDGPAADAA"
     gene            10996..>11843
                     /locus_tag="PKY13_06390"
     CDS             10996..>11843
                     /locus_tag="PKY13_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT79597.1"
                     /translation="MTAASTTIDTPAGAVTATRGSHDAHPAGANYLTTALTTGRRSIL
                     QFFRTPQLLMQGTIQGPLFLLMFRYVFGGAIRTGGALTYVNWMVPGFLTTVILWTGMG
                     GAAGVAEDSSSGVYDRLRSLPIPRSAVMMGRSFADVTLVAWGVLVTGAVGFAVGFRVE
                     GSIGGFLLGLVLILVSGYAFSWVFITMGLVSGNAQAAQGLSMLVIPFSFVSSANVPVQ
                     SMPGWMQPFAANQPVSVVINAVRCLMQGGPEVVGGGIGHSTAYWVVLSLIWSAAILVV
                     FSFLATR"
BASE COUNT         1867 a         3970 c         4130 g         1876 t
ORIGIN      
        1 gggagtggta gcgggtcgcc tcgagcgggt tgggaagccc ggcgaacaca ccggctcccg
       61 agtggcggac gagggatgtc ttgccgtgga cgatctcggg tgcccgcacc acgtcgccac
      121 cgaagacctg gcccatgcac tgcatgccga gacacacacc gaagacgggg acgcgggccg
      181 accactcggc gatcagcacg ttggagagcc cggcatcgtc gggtgtgccc gggccggggc
      241 tgatgaggat gccatcgggg gcgatccgtt ccacgtcgtc gagatcgaga gcgtcgtggc
      301 gccagatcat cggctcggcc cccagctcac ccaggtactg gacgaggttg tagacgaacg
      361 agtcgtagtt gtcgatcacg aggatgcggg ccacgagtcg atgctagcgg cggcctcccg
      421 gcggtccgac cgagatgttt ccggcgcggt ggccgatcgg acggaggcgg atcgtggagg
      481 acaccacctc gttggggcgg tgaccgccta tcctctgcac ctatggccaa accggtgaaa
      541 cgacgtatcg aaggcggcgc cgacgctcag acgagcacgg ggcgcaccac gccgaagggc
      601 acgcgcccgg ccggagaggg tccggcggcg tcgacccgct acacgccgcc gacgccggcg
      661 accaagtacg acatgccgag cccgatgtgg gttcccgtgc tcatgttctc gttcttcggt
      721 atcggcttgc tcgcgatctt cctcaactac accgaggtgc tccccggtgc gccgacccag
      781 tggtacctgc tcggtggact gatgtcgatc ctcttcggca tcgtcaccgc cactcagctg
      841 cgctagcgcc cgttagtcag tcgcggtcga cgcgcgcaat tcccaggttt gtccccagcc
      901 tggggagaac tctgtggaca ctccgagcgc gaggaacacc acgaaacgtg agggtgagcg
      961 gcgggtgaat gccagatggc atgccaccaa acgcatcgag gtcactcgat gggcgtcacc
     1021 agctccatgt cctcgaggca atggcggggt ggctcggggt cttcggacga cgcaacggtc
     1081 atcttgacct cggcgccaca gatgctgcac cggtagacca gcttcacctt gcgcagcttc
     1141 ccggcctcga gcggctcggg cacgggttgc gccagcccgg tgatcatcgc gaggccgatc
     1201 ttgatcagca cggcaccgat gaggacggcg atcgtcgtct ggacgatcga cgtgtcgaag
     1261 gcgagcgtcg ccaccgcgat cgcgatgacg acgcccacca accagaagac cagggagacc
     1321 cgacgcacgt cgatcagtct cccacgagtc gggctcagga cctggccgtc aggattaccc
     1381 gagccgttcg atgatcgtgg cgttggcgag cccgccgccc tcgcacatcg tctggaggcc
     1441 gtagcggcca ccggtgcgct cgagctcgtt gaggagcgtg gccatgagct tggtgcccga
     1501 gccgccgagc gggtgaccga gggcgatggc gccgccgttg acgttggtct tggcgaggtc
     1561 ggcgccggtt tccttggccc acgccaggac gacggaggcg aacgcctcgt tgacctcgaa
     1621 gaggtcgatg tcgtccaagg tgagcccgcc cgagcgggcg atcgccttct gggtcgacgg
     1681 gatgggtccc gtgagcatcg tgacggggtc gacgccggcg agggcgaagg tgtggaagcg
     1741 ggcccggggg gtgaggccga gttccttggc cttctcctcg ctcatgatca acgctgccga
     1801 cgcgccgtcg cagatctggc tggagtttcc ggccgtcacc ttgccgtcgg gcttgaaggc
     1861 gggcttgagg ttggcgagcg actcggccga cgtgtcgggc cggatcccct cgtcggaggt
     1921 gtgcagttcg tcgaggacca cgacgttgcc ggtctccttg tcgcgacgct tcttctgcac
     1981 cgcgacgatc tcgttgtcga accggccctc ggcctgcgcc tgggcagcgc gccgctggct
     2041 ctcggcgccg aacgcgtcca gctcctcgcg gctgatgtcc cactggtcgg cgatcatctc
     2101 ggcggagatg ccctggggca cgaggccacc ctggtcggcg tagcggagac cgacctggtc
     2161 accgaagggg aacccggtgc gcttgatgtc gccgaccgac gcgcccatgg gcacgaggct
     2221 catgacctcg acgccggcgg cgatggcgat gtcgtaggcg cccgccataa caccctgcgc
     2281 cgcgaagtgg gccgactgct gggagctgcc gcactgacgg tcgatggtgg tggcgggaac
     2341 cgactcgggg aacccggcgg cgagcaccgc gttgcggccg acgttgagcg cctggccacc
     2401 gacctgcatg acgcagccca tgatgacgtc gtcgacgagg gcggggtcga ggttgttgcg
     2461 ctcgacgagg gccttgagga cctcggcggc aaggtcggcg gggtgccacc ccgagaggtc
     2521 accgttgcgc ttgccaccac cggtgcggat ggcgtcgacg attactgcag tggtcatgag
     2581 tggactcctg ttgtcacgat tgccggcaac atacttgacc aggcggtcaa gtttttacaa
     2641 ttcgtccccg ccgtgatggg tgacacccgt caccgctacc atttccgccg tctgtcacac
     2701 cgaggggatc caccagcaca tggcaacttc taccgacgag cagctccgcg accaggtcaa
     2761 gggtcagacg gtcgtcaccg agttcctcaa gaccgtggac gcccacgggt ccgaccgcgc
     2821 gctccggtgg atggacggcg aggactggaa ggcgctgacc ttcgacgagc tcgccgacca
     2881 ggtggcgcgg gtcgcgggcg gcttggccga gctcggtatc ggccgaggcg accgggtcgt
     2941 cctcatgctg cgcaacgtgc accagttcca cgtcgccgac ctggccgtcc tcctctgtgg
     3001 cgcgaccccg gtgtcgatct acaactcctc gtcacccgac cagatcgcct acctcgccgg
     3061 ccactgtgag gcgaagctcg gcatcatcga agacgccggc ttcctcgccc gcttcaccga
     3121 ggtcgccgac cagctccccg acctcaccac gctggtgctc gtcaacccgt cccccgacgt
     3181 cgatgtcgag gtcgtcgagt ggtcgtcgct cctcgagcac gaaccggtgg acctcgccga
     3241 ggcggcgaag gtcgcacaac cggaggacct tgcgacgatc atctacacgt cgggtaccac
     3301 cggcaacccc aagggggtga tgctcgacca cttcaacctc tgctggacgc tcgcctgcct
     3361 gcgcgaggcc ttcgagaccg agaccttcgc cgggcagaag gtcgtgtcgt acctgccgat
     3421 ggcccacatc gccgaacggg cgacgagcca ctacggcctg gccatgaacg gctacgaggt
     3481 gtcgtgctgc cccgacccga gcctgatccc cacctacgcc cgcgaagtga agcccaacat
     3541 catgttcggg gtgccgcggg tgtgggagaa gatctacagc ggagtcaccg cagcgctgtc
     3601 cgccgacgag gagaagaagc gccaggtcga cgaagcggtc gccgcggcca tcccgatcgt
     3661 cgagaagatc acctggggta ccgcgaccga cgaggagaag gagacctacg cgttcctcga
     3721 cgccgtcgcg ttctcgacca tccgccagct catcggtctc gacgaactcg atctcgctgt
     3781 cacgggcgcc gctccgatca ctcccgagct gctccagtgg ttccgcgcca tcggcgtgcc
     3841 gctcgcggag atctacggca tgagcgagtc gaccggcccc atcagcttcg ccgctcgtaa
     3901 gccgaaggcc ggcacggtcg gtccggccgt ccccggtctc accctggcga tcgccgacga
     3961 cggtgaggtc atcttccggg gcggcaacgt cttcaagggt tacctcaacg accccgagaa
     4021 gaccgccgaa gcgatcgacg aggacggctg ggtccacacc ggcgacatcg gcgagatcga
     4081 cgacgacggc tacctccgga tcgtcgaccg caagaaggaa ctgatcatca ccgccggcgg
     4141 caagaacatc agccccgcca acctggaggc ggcgctgaag acgatcccgc tcgtcggcca
     4201 ggccgcggcg atcggcgaca accgcccgtt cgtcgccgcg ctcgttgtgc tcgatcccga
     4261 ggcggcgccc gtgtgggccg cgcagcacgg actcgagggc aagtccctcg acgagctggc
     4321 cgaggagccg gccgtgatcg acgagatcaa cgctggcctc gccgacgcga tgagccgctt
     4381 caacaacgcc gagcgcgtca agcgcgtcaa ggtcctcggc gaggagtggc tgcccgattc
     4441 cgacctcatg acccccacgt ccaagctgaa gcggcggggc atccacgcga agttcgcgga
     4501 ggagatcgag gcgttctatg gcggctgatg cccgtcggcc accgaaggtg gcgatgttgg
     4561 tgtcacggtt cccgaaggtg accgagacgt tccagctcag ggagatggtg gcgctcgaga
     4621 agatgggcgt cccgatcgag ctgtacgcga tcacccatca cgacgacggt ggaacggtgc
     4681 aggccgaggc cgctgcactc gacgcgcggg cgaactactt cccgcgcatg tcgtgggaga
     4741 tcctgaaggc gcagttcatc tggctgcgtc gcaatcccgc cggatacgtc aaggcgtgga
     4801 agtggagcat cgcttcgaac ctcggggccc ccgagttcct gctgcgcagc ttcctgctgg
     4861 ttcccctggc cgccgcgatg gcgttgcgaa tggaacgcga cggcgtcgag cacgtgcacg
     4921 cccacttcgc gacctacccg acccacgcgg ccctcgtcat caagtggcta gccggcatcc
     4981 cgttctcgtt cacggggcac gcccacgaca tccaggttcg tcaggacggc atcggggcca
     5041 agatcgagga gtccgagttc ttcgtctgtt gcacccgcta cagcctggac tcactccgat
     5101 cgctctacgg cgacatcgtc gacgagaagg gcaacgtcgt ctaccacggt gtcgacctcg
     5161 atcagttcac gttccgtgag ccccactccg acgacggcga gcgtgcgttc cgcatggtgt
     5221 gcgtcgcgac gttcgaggag ttcaaggggc acacctacct catcgacgcc gtgcgattgc
     5281 tcgccgagcg cggcgtcgcc gtggagctgg tgctcatcgg cggcgatccg ccacgccggt
     5341 cgaccctgca ggacgacatc cgtgcccagg tcacggccgc cgggctcgac gattcggtgc
     5401 ggttcctcgg caaggttccg tcggccgagg tgcggacgtg gatcgagtgg tccgacatcg
     5461 gggtgctggc gtgctgtcgc caacccgacg gtcagatgga cgggcttccg aacttcctca
     5521 ccgagtgcga ggccatgggc cgaccggtgg tgtcgacgcg gcaggcgcca gtgatggaac
     5581 tgatcgtgga cggcgagaac ggcaagttgg cccggacccg cgacgccgtc gagctcgcgg
     5641 acgccatcga gtcgatgcgt cggctcgatc ccgaggcccg ggcgaagatg ggcctcgccg
     5701 gccacgagcg cgtgcagcgc gagcacgacg tcaccgtcaa caccgcagcg ctgtacgaca
     5761 tctacctgga tcgggtcggc aggaactaag tcgagatcgg tgggtcgccg agccagcgtt
     5821 gcccaacgct cgactggatc gacgccgagc cctcaccgac gtagcggaac accttccaca
     5881 tcgctgtgcc gcgcatctcg tccgcgagat ccggcaggtc gagcacgaag cggatgacgc
     5941 tggcgtcgtc gttggggcga cggccagcct tgaggtcctc catggcggcg atcatcgcgt
     6001 ggttgtccga tcgcacgagc aggtggaacg cggtgatgcc gcgagtgagg gcgacggccg
     6061 cgagcgcctc gagcatgatc gagccgatac cacgccgatg gtggtcgtcg gcgacggcga
     6121 cggcggcctc ggcggcggac gggtcgctct tggagcggat gtaccgggcg acgccgacgc
     6181 cgagggcggg atcgtcgggc gtcgcgatcg tcgcgaccca cgcgaagtgg tcgacgtagt
     6241 cgatgtcggt gaagtaccgg agctgcgccg cgctcagatg gtcgagcggc gagaagaagc
     6301 ggaggaaccg actctccggt gacaaccgcc ggaacccctc agcgagcatc ggcccgtcgt
     6361 cgggccggat ggggcggagg ctgaggacag cgccgtcggg cagctgcgtc gtgaccgcca
     6421 ggtgtgcgac ctcgtcgggc agcatcgttc cgcggcccct cagcgccggc cgctccagga
     6481 accgacgacc ggatcgacct tgatgaaccg cggatcgatc tcgccggcgg tgagcggacg
     6541 gacggccaga tcgatcgcgg tccacatcgt gtgggccgat ggcaggaaga ggatgggcag
     6601 gaggccggcg gccgagagcg tggcgatgag cagtggcaca acggggatat cgggttgcgt
     6661 cgcgatcgac cctcccagga ggacgacgaa cgtcactgcg aacgtcacga ccgtgttgag
     6721 cccgatgtag ccgatcgaat ggccgaagat ccgctcgaac ttcaacgagc actgcgggca
     6781 ctcgttgagc agggtggcgt aactgctgtg cgtcttgcgg ccaccgcacg ccggacagcg
     6841 tcgcaggaag ccgcgccaca gcattttggt gttggagacc ccgagttggg tggaggtcgg
     6901 gaccggaggg tggaccgtca tgctcgtatt gtgccgcgtt tccgcgcctc gttggtcccg
     6961 gaggtcgtcg gttccgccga cagatgacgc gctcccctga cgtgggcccg acgtcgacga
     7021 cggtcgtggg tgtcgcgtcg cggtagtttc gcccgatgga ccaacggacg atcgtcatca
     7081 cgggcgccaa cacgggcatc ggcctcgaga ccgctgtgtc gctcgcgtcg gcgggggagc
     7141 gcgtgatcat cgcgtgccgc aacccggcga aggccgatgc agccgtcgcc gagatctcgg
     7201 cgcgggcggc ctcgtcggac gtctctgccg tcccgctcga cctcgcgagc ctggcctcgg
     7261 tccgagcgtg tgccgccgaa ctcggtcggc gctgtgagcg aatcgacgtc ctcatcaaca
     7321 acgccgggct catccagtcg ggtcgggaaa cgaccgtcga cgggttcgag acgaccttcg
     7381 gtgtcaatca cctcggccac ttcctgctca ccgacctcct cggcgacctg gtgaaggcgg
     7441 ctccgacgcc gcgggtcatc aacctcgcgt cggtcgccca ctggtttgcc gtcggtggcc
     7501 tcaacttcga ggacctgcaa tcggtccgct tctacaacgt gtgggttgcg tacggtcggt
     7561 cgaagctggc gaacatcctc ttcaccgacg agttggctcg ccggtggcgc gacgccggcg
     7621 tctgcgtgtc gtcggtgcac cccggtgtgg tgcgcagcgg gttcggccag gacggcgaca
     7681 ccagaggcat cgccgacaag ctgatcgaca tctccaagcc gttctcgatc acacccgacc
     7741 gtggcgccga cacgtcggtc tggttggcga ccgcggccgc cggtgccgat ctcgaccgca
     7801 gcggcacgtt ctgggcgagc cgcaagccat ccctccgggc tccgtgggcc cgtcgtcgga
     7861 ccgatgcggc ccggctgtgg gccgagagcg aggagctgat cgcgtcggtc agctgaccga
     7921 gacgctcagc gcaggggcgg ttcgctcgac caggggaagt tgatccactg gtcggtgtga
     7981 cgccacacgt agtcggcgga gaagatggag cggggcttct cgtagatgac gagggagcgg
     8041 atctcggcca cgacgggtgc gcaggtgtcg acgacgagct tgagcgtctc cccggtgtcg
     8101 gcgacgtcgt cgacgatgag cagcttggtg tcggcgagat cggccaggtt gagggccggc
     8161 gggagcatga cgggcacctc gaggcgctcg tcgatgtcgg tgtagaactc gacgttgacg
     8221 actccgatgt tcttgcagcg cagcgcgtag gcgaccgctc caccgatgag gaggcctccc
     8281 cgggcgatgg cgacgatcca gtcgggttcg aacccgctgt cgaccacctg ttggcagagt
     8341 tgccgcgtgg ccaccccgaa cgttccgtag tcgagctctt cgcggtctcc gtcgacgatg
     8401 gcttccatgc gccggttcta gtcggcaccc cgcgcggacg ccaacggcac ggacgcggcc
     8461 gcgaccccgt gggccacgaa ggtcgaggcg agggtggcga acgcgtcgaa gccgcagaac
     8521 ctccggacga gcgcgagctc ggtcgcgacg aagtcggcgc cgtggggttc gcgatcgggc
     8581 gagaccaggt gcgacagctc gtggaggacg gtgagggcgt tccaactgcc gtcacggatg
     8641 aggatggtcg cccggtcggg aagcgcgacc gacgcccggg cggagcggct ccgtcggtcg
     8701 aggacgacgt cgagcggcgc gtcggggaag aggtcgaggt aggcgggatc ggcgaggatg
     8761 gcttcgacga aggcctcgac gtcggtccat cggcggagcc gccggccgat gtcgtcgagc
     8821 accgagtcct cggcggcgta gacgagatgg cgctgggggt cgttcgtatc gatccgctcg
     8881 tcgcgggcat tcggggccac gtcggcggag ccttcagttg ccctgtccca gcgcctggcg
     8941 atggccgatg cggcgggcgc cgagatcggc cgtccggcct gctgacgtgc cggcgcggcg
     9001 gccggactcc gacgtgcccg agctgacccg ggatgtgcgg atgcgggggt agcgctggcg
     9061 gaactcgtcg tcgacagcgg cagagcggtc gcggaggacg acggcggcag actgctcgcc
     9121 ggcctcgggg tggcgttcgc gatcggtctc ggccgtcgct cgggcccggt cggcgcggag
     9181 ccgttcggtg atggtggcgg tgaaccccat gatgaaactg cggcgccacg ccgcgacctg
     9241 cgacgcgggg cgcccagcgg ggccgggcag cggggcggtc atggccgacg tgagttggag
     9301 gaacaggctt gtgaccaggg tctcgacgag cgacagatcg gacgcgaacc cgatgacgga
     9361 catgtgctcg gtcgcgccgt cgtcggctcg gccgaggcgg agcgcctggc acccgtaggc
     9421 gttggcgacg gcattgacga gcacggcctt ctgcgcggtg aacggacgat ggaggaccag
     9481 gcgaatctcg gtgggcgtac cggacgtgtc gcgagcaccc gcccacagca tcgcctcgtc
     9541 gaccgcgtac cgcgccatga gttcggacgc cttggcggtg aaggcttcgg cctcggccgc
     9601 gaacgtcgtg gcctcggctt tggccagcag cgcccggatg gtggccaggc gaccgtcggt
     9661 gtcagtggga ggtggcatcg ccccagtctg gccgaccgca ctgacactga atcgaggtcc
     9721 gcaaccgtca cgtgggagat cgacgaggag accgattcgc tgtgctccct caccgtgacc
     9781 cacgatgtga ccggcgcacc gttccacggc gccgcaaccg tcgggcgttg cccacggcaa
     9841 gctcaacgag ggcggcggcg gttggccgtt cgttctcagc gacctcgagt ctctgctcga
     9901 gaccggcgcg gccttcgagt tctgaggaac ctcaccgatc agttctcgac ctcggcgtcg
     9961 tcgtagacac gacgatcgac gccgtcaccc ccatccaagg agtcacgcca catgtcagcc
    10021 atcatcgagg cccgcggcct cacgaagtcg ttcggggaca cccacgccct ggccggcctc
    10081 gacctcaccg ccgaggccgg gcgcgtcacc gcattgctcg ggcccaacgg cgctgggaag
    10141 accacgttca tcagcgccgt cagcacactc ctccggcccg actcgggcga gctccgggtc
    10201 gccgggatcg acgtgctggc cgaacccaag aaggtgcgtc aggtcatcgg tctcgcgggg
    10261 cagtacgcct cggtcgagcc ggcgatgacg ggcatcgaga acctcgacat ggtcgcccgc
    10321 ctgttcggcc tcgaccgggt caacgccaag cgcgctgccg gcgaggtgct cgagcgcatc
    10381 ggcctcaccg aggccggcac gcgcgtggtc cgcgactact ccggcggcat gcgccgccgg
    10441 ctcgacctcg gcgccagcct ggtcgggcgc ccgcgactcc tgctgctcga cgagcccacg
    10501 accggtctcg acccgcgcag ccggagcgag ctgtgggacg ccatccgcga cctcgtcagc
    10561 gacggtaccg acgtgctgct caccacccag tacctcgaag aggccgatca gctcgctcgc
    10621 gatgtcgtga tcgtcgacca cggacgagtc atcgcgtcgg ggacccccga tgagctgaag
    10681 gaccaggcgg gccgcgacgt catcgaggtg cgcccccgct cggccggcga cctcgccgcc
    10741 gtcgccgaga tcctggcgtc ggtcgccgcc gaggagccgc gcagcgacga cgacagtcat
    10801 cggatcacgg cgaccgtcga cggcggtgcc ggcgacctcg ccaccgtggt gcgcctgctc
    10861 gacgagcggg gcctgcaggt cgacgacatc ggcctccgtc gtccgaccct cgacgaggtg
    10921 ttcctcacct tgaccggcca acccatcgac ccgagcgacg gcccggccgc cgacgccgcc
    10981 tgaacggaga ccaccatgac cgcagccagc accacgatcg acacgccggc gggcgccgtc
    11041 accgcgaccc gcggttcgca cgacgcgcac ccggccgggg ccaattacct gaccaccgcc
    11101 ctcacgaccg ggcgtcgcag catcctgcag ttcttccgca ctccgcagct cctgatgcaa
    11161 gggacgatcc agggtccgtt gttcctgctc atgttccgct acgtgttcgg cggagcgatc
    11221 aggaccggcg gtgccctgac ctacgtgaac tggatggttc ccgggttcct caccaccgtc
    11281 attctctgga ccggcatggg cggggcggct ggcgtggccg aggattcctc atcgggcgtc
    11341 tacgaccggc tccgttcgtt gcccatcccg cggagcgctg tgatgatggg gcggtcgttc
    11401 gccgacgtca ccctcgtcgc gtggggtgtt ctggtcaccg gggccgtcgg gttcgccgtg
    11461 ggctttcggg tcgagggcag catcggcggc ttcctcctcg ggctcgtcct catcctggtg
    11521 tccggctacg cgttctcctg ggtcttcatc acgatgggtc tcgtgtcggg caacgcccag
    11581 gccgcacaag gcctgtcgat gctggtgatc cccttctcgt tcgtgtcgag cgccaacgtg
    11641 ccggtccagt cgatgccggg ctggatgcag ccgttcgccg ccaatcagcc ggtcagcgtc
    11701 gtcatcaacg ccgtgcggtg cctgatgcag ggcggtcccg aggtcgtcgg cggaggcatc
    11761 gggcactcga cggcctactg ggtcgtcctg agcctcatct ggtcggccgc catcctggtc
    11821 gtcttctcgt tcctcgcgac tcg
//
LOCUS       DAOSOA010000070        11604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5533_length_11604_cov_6.308425, whole genome shotgun sequence.
ACCESSION   DAOSOA010000070 DAOSOA010000000
VERSION     DAOSOA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11604
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5533_length_11604_cov_6.308425"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PKY13_06395"
     CDS             <1..1274
                     /locus_tag="PKY13_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79598.1"
                     /translation="TLLVTSVAALLLETPGRTNQVARRIAAGFLGGLACLTRLDSAVL
                     VGTWFLLVLWYEWRQDAPFRPVRVLTAAALLGVPLAAVLVPWLVWKYDYYGDLLPNTL
                     TAKSGGFIVPFLYGVFYLLAFFASYFGFLLVKRYRKYRSEWFANPQAKLVFIPVLVWL
                     LYTCVVGADFMEYRFMVPIIPILAMLAAYLLNTFTKVRSQAILVAALLFGSGLHMIAP
                     TVVAYPVLTFKELNHWPSESKTAWIGLGKYLRDEFPGGELAAGQPKIAIQPLGVISYY
                     SELPVVDMLGLADKEIATDGIQIPLYYPGHVRMATVQQLVDKDVTLIAGLPTFVNDDP
                     DRADYRLSELAAMYSTANLQDLPEEATIVEAPIVPGKVWFMIYLEPNDKVDALIESGE
                     WRQLPISRTCKDSDLNVITRIISEDTCKGLL"
     gene            complement(1271..2494)
                     /locus_tag="PKY13_06400"
     CDS             complement(1271..2494)
                     /locus_tag="PKY13_06400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT79599.1"
                     /translation="MNRAGVEQVEPTRTDGPFVSRAPKLGYFPPFDGVRGGAMIMVLL
                     AHLSYSQFASFAASVDMFFVISGFLITTLLLEEHRKTDRVDMGEFYWRRAFRLFPMLY
                     ATLAATVLGALATGKAQLFKEAWQDALAAGLYVYHVVHPVGIEINTSQFPHHRPLVQL
                     WSLSVEEHFYLIAAVLTVVVVRHRLPKLLIAVFMGIWLFVGVARLTGHVGPRLMWYQR
                     PDSLLIGVCVAYLHALMPPELSEKAARLLRAVGLLALGVIVDVVFIGTGLAPLRIRFQ
                     FSPFDGISQLRDGWYWGRFGFSMVNISLGVLLLAMVRNGEWIVSRFMSWRPLREVGIR
                     SYCIYLIHVPLAVIMFEQWGERDSKGEFKPDPVLFLSYLVALVVLTQLSHKYLEQPLM
                     RKGKELRSRQKAAAS"
     gene            complement(2535..3185)
                     /locus_tag="PKY13_06405"
     CDS             complement(2535..3185)
                     /locus_tag="PKY13_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOT79600.1"
                     /translation="MQIEVWSDVICPWCYIGKRHLELALAEFEHADEVEVIWRAFELD
                     PGAPTDSVADLASTLAAKYGTDRDGAIAMMDRVASVAEGVGLHYRFDLAHRSNTFDAH
                     RVIHLALDEGGAALQDAVKERLLAGYFTEGADLADRNVLVKLAAEAGLDAETVTGMLA
                     SDALATEVRADEAQAREYGISGVPFFLIPGAGGVSGAQPPDRLLLMLQRAWDKIGA"
     gene            complement(3175..4008)
                     /locus_tag="PKY13_06410"
     CDS             complement(3175..4008)
                     /locus_tag="PKY13_06410"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT79601.1"
                     /translation="MHPVIADQELPHFLRARRGAVQPADVGLPPGGRRRTPGLRREEV
                     AMLAGVSVSWYTWLEQGRPINVSLEVLDALARVLRLDPAERDHLVELAGHSIRHTGTP
                     AGADVPEWAERLLSSVDPAPAYLLGPTWEYLAWNAAMEHLFPPLADLPTNERNLVWVM
                     FAMPETRDLIVDWSTEARRVLSQFRADITPWRDEPAVVDLVARLRSVSSEFDEWWERH
                     DVAGFETRLRRFNHPTDGQLTFEYQQLIPAGRTDLRFVVQLPISDHPTTEHEEPDDDA
                     D"
     gene            4091..5278
                     /locus_tag="PKY13_06415"
     CDS             4091..5278
                     /locus_tag="PKY13_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT79602.1"
                     /translation="MESAATLLSARARSSTSSVIRDLLRLVDRPDMLSLAGGLPAAEF
                     LPVERLAAATERVISPRSLQYGPTEGMLELRTVVAHRLGAAVDDIVICTGSQQGLDLV
                     ARALLDPGDVVVVEHPSYLGSLQSYRANGAVLHPVEGDDEGLRVDRLAADLAAGLRPK
                     LVSVVTDFANPTGATLAHDRRVVLVELAARYGFVVLEDNPYGELRWAGEHTTSIGSIA
                     AAAGGSDRVVTLGSASKVLSPGLRLGWMRGPAWLLDAVVKLKQSADLHTSSLDQLLAA
                     DVLGDPAFMADHLERIRTSYRDRSTALVDAIEELVGDRVEARRPDGGMFLWARLRDGS
                     STSDLFDRALDAGVSFVPGAAFAPDDAPSDRMRMCFTTLSAGDLHRAVGRLGEALGAV
                     GPR"
     gene            5372..5572
                     /locus_tag="PKY13_06420"
     CDS             5372..5572
                     /locus_tag="PKY13_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT79603.1"
                     /translation="MATGTVKFFNAEKGFGFIAREQQDDIFVHYSNIQGSGYKSLEEG
                     QQVEFDTAPGRKGEEAQNVRVI"
     gene            5681..5980
                     /locus_tag="PKY13_06425"
     CDS             5681..5980
                     /locus_tag="PKY13_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79604.1"
                     /translation="MGVQRTSFEKLQRDRAKKAKAAAKRDKRINGVPPTTEDPDTTVD
                     PDEVAALMGDEDKQLSAAELLELIEKIHQQFENEDITFEEFEEKKIELFARLPID"
     gene            6028..7038
                     /locus_tag="PKY13_06430"
     CDS             6028..7038
                     /locus_tag="PKY13_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT79605.1"
                     /translation="MKLSKPAGGLIGLGMVALAGVSAVKVAGTRIRHHQDPELDALFA
                     SAGELAHHHLPTADGGELHVVEAGAGRPVVLLHGVTLQWWVWSALISLLRDRYRVVAW
                     DMRGHGESRAGRDGVSLEATASDLAMLLRELDLDDAILVGHSMGGMTLGHFVGRHSDE
                     LAARVSALCFVATSGCATDKSFSRGNLKTLYGLAGRALLAGLRRPSPRYPWHDNDLSA
                     VLLRTAFGPSATADMIDAVRRMSADMATASMIEAGNALAAHDVRQVLPFVDMPTTVVV
                     GDADRITPPGHARELAELIPGATLVELEGVGHQIMQESPVALAGIIDDLDGRAPSLVP
                     VA"
     gene            complement(7039..8847)
                     /locus_tag="PKY13_06435"
     CDS             complement(7039..8847)
                     /locus_tag="PKY13_06435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOT79606.1"
                     /translation="MPREFAHLPALDGLRALAVLAVVLYHADVVLVGGGFLGVEVFLV
                     ISGYLIASLLLHQRDVSGGIALRRFWLGRVRRLVPAVAVLVLGCIAAAAVFAPDALRQ
                     LRTDLPGAVLYVSNWTQILHDESYFLAAGRPPLLRHLWSLAVEVQFYAVFPFVMAVAG
                     ARLRRRHLFVGAGLGAVASALLMAALYHPSADPSRVFFGTDTRASGLLVGVALAAIWP
                     AQRFRPTRVPAAAQRTLDITGGAALVVLVGTFLWVNEFDPFVYRGGFLLVDLATMVLI
                     AAAVHPAGRLVGMLGIRPLAWIGQRSYSLYLWHWPVIVLTRPDLDVDIHGTALLILRL
                     VLSFALAELSYRFVEAPFRSGALGRVVGAYRRPSSPQERLRARWFVGFASAALATSAV
                     ITAWPDLPDADSGLADQPTEVIDVAPTAAPTTAVVSTTADPTAPITPPTTAAPTTTTI
                     PVGVLAVGDSVMAGAVDPMRTLIGSLTVNAKISRACGEAVRIVRASLADGSLPQTVVV
                     HMGTNGPCTETELDDLVALTADRRLVLVDVAAPRPWEHLANERIAAAVARAPGVGLAD
                     WKSRSDGHPEWFVADGVHLTPAGRLAYAALVSEAIG"
     gene            complement(9031..10620)
                     /locus_tag="PKY13_06440"
     CDS             complement(9031..10620)
                     /locus_tag="PKY13_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT79607.1"
                     /translation="MTVPSTNSGQRPGNVLFITVDQWRGDSLSCVGHPLVRTPNLDAL
                     AADGVLFARHFANTAPCGPSRASLYTGMYAQNHRSILNGTPLDARFTNVALLAREAGY
                     SPALFGYTDTSVDPRTVGPDDPRLSNYEGVLPGFDPIVNDPEVYGSLVWADWLRRQGV
                     DVPANPRELYWPIPDFPGSAEHGATWAPTHFAAEHTETAFMVSELIDWIDGRTTAEWD
                     AEPFFVHASFIRPHPPYRNPVGYHDLYSADDVPPFVGHASRDDEIGAHPLNAVSIPLA
                     GVGAPVDDLDRRQLRATYHGMQTEVDDQLGRLFGHLRERGLDRNTLVVITSDHGEMGG
                     DHWLTEKLGYWDESYHIPLIVRDPRPDADATRGLQVGEFTESVDVLPTILEWIGVPIP
                     DQVDGRPLTRFVHAGVAPPHWRDAVFWEWAFNDPEHRFSEFLLNAPAEHCSLNVVRTD
                     EWKYVQFAGAAEAMPPLLFDLVNDPHQLVNLADDPAHTPQVADCLRRLVRWRMQHDER
                     TMSTYHLSESRGLLHVADPRR"
     gene            10664..11602
                     /gene="mmuM"
                     /locus_tag="PKY13_06445"
     CDS             10664..11602
                     /gene="mmuM"
                     /locus_tag="PKY13_06445"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HOT79608.1"
                     /translation="MDRDRGLADLGGRTDLAGLMADHVVRLDGGLSTALEERGHLLTD
                     SLWTARLLLEAPAEVTAAHAAFVAAGAEVLITASYQVSEQGFMAAGLSAADASNALRA
                     SVGAARAAGGALVAASVGPYGAILHDGSEYRGRYGLTVDELVAFHRPRLDVLLSARPD
                     AIAVETIPDLDEAAALAELLGALPAGGPPAWIAFSCAADGRLHSGHDVADAAALVAAV
                     PGVVAVGVNCTRPADVATALERIRSATELPLVVYPNGGGSWDAEHNCWHDQEFAADDL
                     VAEWLDLGVRLLGGCCGYGPSALAALGSVVAALERS"
BASE COUNT         1806 a         3844 c         4205 g         1749 t
ORIGIN      
        1 agacgctgct cgtcacgagc gtcgcggccc tgctgctcga gacgcccggg cgcacgaacc
       61 aggtcgcacg tcgcatcgcc gccggcttcc tcggaggcct ggcgtgcctg acccgcctcg
      121 actccgccgt gttggtgggc acctggttcc tcctcgtcct ctggtacgag tggcgtcagg
      181 atgcaccgtt ccggccggtt cgtgtgctca ctgcggccgc gctcctcggt gtgccgctcg
      241 ccgcggtgct cgtcccttgg ctcgtctgga agtacgacta ctacggcgac ctcctgccca
      301 acacgctgac ggcgaagtcg ggcggcttca tcgttccgtt cctctacggc gtcttctacc
      361 tgctcgcgtt cttcgcgtcg tacttcgggt tcctgctcgt caagcgctac cgcaagtacc
      421 gctccgagtg gttcgcgaac ccgcaggcga agctggtgtt catcccggtc ctcgtgtggt
      481 tgctctacac ctgcgtggtc ggtgcggact tcatggagta ccgcttcatg gtcccgatca
      541 tcccgatcct cgcgatgctc gccgcgtacc tcctgaacac gttcaccaag gtccgctcgc
      601 aggcgatcct cgtcgcggcg ctcctcttcg gatcggggct ccacatgatc gccccgaccg
      661 tcgtggcgta ccccgtgctc accttcaagg agctcaacca ctggccgagc gagtcgaaga
      721 cggcctggat cggcctcggc aagtacctgc gcgacgagtt ccccggcggt gagctcgccg
      781 cgggtcagcc caagatcgcc atccagccgc tcggcgtcat ctcgtactac tccgagctcc
      841 ccgtcgtcga catgttgggt ctcgccgaca aggagatcgc gaccgacggc atccagatcc
      901 cgctgtacta cccgggccac gtgcgcatgg cgacggtgca gcagctcgtc gacaaggacg
      961 tgacgctcat cgccggcctg ccgacgttcg tgaacgacga tcccgaccgg gccgactacc
     1021 gcctcagcga gctggcggcc atgtactcga cggccaacct ccaggacctc cccgaggagg
     1081 caacgatcgt cgaagccccg atcgtgcccg gcaaggtctg gttcatgatc tacctggagc
     1141 cgaacgacaa ggtcgacgcg ctcatcgagt cgggcgagtg gcgccagctc ccgatcagcc
     1201 gcacctgcaa ggacagcgac ctcaacgtga tcacccggat catctccgag gacacctgca
     1261 agggcctgct ctaggaagct gcggccttct ggcgggagcg gagctccttg cccttccgca
     1321 tgagtggctg ttcgaggtac ttgtgcgaca gctgcgtgag tacgacgagt gcgacgaggt
     1381 aggagaggaa cagcaccggg tcgggcttga actcgccctt ggagtcgcgc tcgccccact
     1441 gctcgaacat gatgacggcc agcggtacgt ggatcaggta gatgcagtaa ctgcggatgc
     1501 cgacctcccg gaggggacgc caggacatga agcgggagac gatccactcg ccgtttcgca
     1561 ccatcgccag cagcagcact ccgagcgaga tgttgaccat cgagaagccg aagcgacccc
     1621 agtaccaccc gtcgcgcagt tgcgatatgc cgtcgaacgg cgagaactgg aaccggatac
     1681 gcagcggtgc gagcccggtg ccgatgaaca cgacgtcgac gatgacaccg agcgcgagga
     1741 gaccgaccgc tcgcaggagc cgagcggcct tctcactcag ctcaggcggc atgagggcgt
     1801 gcaggtaggc gacacagacg ccgatgagca ggctgtcggg gcgctggtac cacatgagcc
     1861 gagggccgac gtggccggtg aggcgggcga cgccgacgaa cagccagatg cccatgaaca
     1921 ccgcgatcag gagctttgga agccggtggc ggacgacgac caccgtcagc acggcggcga
     1981 tgaggtagaa gtgctcctcg accgacagcg accagagctg gaccaacggc cggtggtggg
     2041 ggaactggct cgtgttgatc tcgatgccga ccggatgcac cacgtggtac acgtacagcc
     2101 cggcggcgag cgcgtcctgc cacgcctcct tgaacagttg cgccttgccc gtggcgaggg
     2161 cgcccagcac ggtcgcggcg agcgtcgcgt agagcatggg gaagaggcgg aaggcgcggc
     2221 gccagtagaa ctcgcccatg tcgacgcggt cggtcttgcg gtgctcctcg agcagcaggg
     2281 tggtgatgag gaacccgctg atcacgaaga acatgtcgac gctcgcggcg aacgaggcga
     2341 actgcgagta cgagaggtgc gccagcagca ccatgatcat cgccccgccg cgcacaccgt
     2401 cgaacggtgg gaagtagccg agcttcggag cccggctgac gaacgggccg tcggttcgcg
     2461 tcggttccac ctgttcgacc cccgcccggt tcatcagggt gagggtatcg gttcacggcc
     2521 ggttcgggcc gggttcaggc gccgatcttg tcccaggccc gctgcagcat cagcaacagg
     2581 cggtccgggg gctgtgcacc cgacaccccg ccggcgccgg ggatgaggaa gaacgggacg
     2641 cccgagatgc cgtactcgcg ggcctgcgct tcgtcggcgc ggacctcggt cgcgagtgcg
     2701 tcggacgcca gcatgccggt gacggtctcg gcgtcgagcc cggcctccgc cgccagcttc
     2761 acgaggacgt tccggtcggc gaggtcggca ccctcggtga agtacccggc gaggagtcgc
     2821 tccttgacgg cgtcctgcaa cgccgcccca ccttcgtcga gcgcgaggtg gatgacccgg
     2881 tgggcgtcga acgtgttcga ccggtgggcc aggtcgaagc ggtagtggag cccgacgccc
     2941 tcggccaccg atgcgacgcg gtccatcatc gcgatggcgc cgtcacggtc ggtgccgtac
     3001 ttcgccgcga gggtggatgc gaggtcggcg acgctgtcgg tcggggcgcc ggggtcgagc
     3061 tcgaacgccc gccagatcac ctcgacctcg tcggcgtgct cgaactcggc gagtgcgagt
     3121 tccaggtggc gcttgccgat gtaacaccac gggcagatca cgtcggacca cacctcaatc
     3181 tgcatcgtcg tcgggctcct cgtgttcggt ggtcgggtgg tcgctgatcg ggagctggac
     3241 gacgaaccgg agatcggtgc gccccgccgg gatcagctgc tggtactcga aggtcaactg
     3301 cccgtcggtg ggatggttga acctcctcag tctggtctcg aagccggcga cgtcgtgacg
     3361 ctcccaccac tcgtcgaact cggagctgac cgagcgcagg cgtgccacga gatcgacgac
     3421 ggcgggctcg tcccgccagg gcgtgatgtc ggcgcggaac tgcgagagca ctcggcgggc
     3481 ctcggtcgac cagtcgacga tgaggtcgcg ggtctcgggc atcgcgaaca tcacccacac
     3541 gaggttgcgc tcgttggtcg gcaggtcggc gagcgggggg aagaggtgct ccatggcggc
     3601 gttccacgcc aggtactccc acgtgggccc aagcaggtag gcgggggccg ggtcgaccga
     3661 cgagagcagc cgctcggccc attcgggcac gtcggcgccc gccggtgtac cggtgtggcg
     3721 gatcgagtgg ccggccagtt ccaccaggtg gtcgcgctcg gcggggtcga gccggagcac
     3781 ccgggcgagc gcgtcgagca cttccaggct cacgttgatg ggtcgcccct gttcgagcca
     3841 ggtgtaccaa ctgaccgaga caccggcgag catcgcgacc tcctcgcggc ggaggcccgg
     3901 cgtgcgacgg cggccccccg gcggcaaccc gacgtcggcg ggctgcacgg cgccccgacg
     3961 ggcgcgaagg aagtggggca gctcctggtc cgcgatcacg gggtgcattc gagcacagcc
     4021 aacctgtgac tcgcggtccc atgttgggtg cgctctgtcg gtcgggcccc aaccgtggtc
     4081 ggatccggcg atggagtccg ccgccaccct gttgtccgcc cgcgcccgtt catcgacgtc
     4141 gtcggtcatc cgcgacctgt tgcggctcgt ggaccgtccc gacatgttgt cgctcgctgg
     4201 cggtctgccc gctgcagagt tcctgccggt ggagcgcctc gccgcggcga cggaacgagt
     4261 gatcagccct cgctcgctcc agtacggacc gaccgagggg atgctcgagc tgcgcaccgt
     4321 cgtcgcccat cggctcggcg ccgcggtcga cgacatcgtc atctgtaccg gttcgcagca
     4381 gggactggac ctcgtcgccc gggccctgct cgaccccggc gatgtcgtgg tcgtcgagca
     4441 cccgagctat ctcgggtcgc tccagtcgta ccgcgccaac ggggcggtcc tgcacccggt
     4501 ggagggcgac gacgaggggc tccgggtcga ccggctcgcg gcggacctcg ctgcgggtct
     4561 gcgccccaag ctcgtctcgg tcgtcaccga cttcgccaac cccaccggtg cgaccctggc
     4621 gcacgaccgg cgcgttgtcc tcgtcgagct tgcggcgcga tacggcttcg tcgtgctgga
     4681 ggacaacccg tacggcgagc tgcgatgggc cggtgaacac accacgtcga tcggctcgat
     4741 cgccgccgct gccggcggca gcgatcgggt ggtcacgctc ggctcggcgt cgaaggtcct
     4801 gtcgcccggc ctccggctcg ggtggatgcg cgggccggcg tggctcctcg acgcggtcgt
     4861 gaagttgaag cagtccgccg acctgcacac gagctcgctc gaccagctcc tcgccgccga
     4921 cgtgctgggc gatccggcgt tcatggcgga ccatctcgaa cggatccgca cgagctaccg
     4981 cgaccggtcc accgcgttgg tcgatgcgat cgaggaactg gtcggcgatc gggtcgaggc
     5041 acgccgaccc gacgggggca tgttcctgtg ggcgcggctg cgcgacgggt cgtccacgtc
     5101 ggacctgttc gaccgtgcgc tcgacgccgg cgtgtccttc gtgccgggcg cggcgttcgc
     5161 ccccgacgac gccccgagcg accgaatgag aatgtgcttc acgaccctgt cggccggcga
     5221 tctgcaccgc gccgtcgggc gtttggggga ggcgctcggg gcggtcggac cacgatgatc
     5281 ggaccccgac gggcctttcg gcggtcgaac cccctaagct gtccgctgtg gtcgtcaaac
     5341 agggggccac gcaagtagaa gagagagtac gttggcaaca ggcactgtga agttcttcaa
     5401 tgctgaaaag ggcttcggtt tcatcgcacg tgagcagcag gacgacatct tcgtccacta
     5461 ctccaacatc cagggcagcg gctacaagtc gctcgaagag ggccagcagg tcgagttcga
     5521 cactgcgccg ggtcgcaagg gcgaagaagc ccagaacgtc cgagtcatct gagacttcgc
     5581 gtgtgacggg gccgccggcg acttcgccgg cggcttcgtc atgctcgtga gatccacagg
     5641 aagtttctga ctggctgaag tcgtgtgaag ggagcccgag atgggcgtac aacgaacgag
     5701 ttttgaaaag ttgcagcgag accgcgccaa gaaggcgaag gcagctgcaa agcgcgacaa
     5761 gcgcatcaat ggtgtgccgc cgacgacgga ggatcccgat accacggtcg atcccgatga
     5821 agtcgcagcg ctgatgggcg atgaggacaa gcaactctcc gccgccgagc tgctcgagtt
     5881 gatcgagaag atccaccagc agttcgagaa cgaggacatc acgttcgaag agttcgagga
     5941 aaagaagatc gagctgttcg cgcggcttcc gatcgactga tccccgagcc ccacggaccg
     6001 accacaacga gatcgagagg gtcatcggtg aagctcagca agcccgccgg cggcctgatc
     6061 ggtctcggga tggtcgcact cgccggtgtg tcggccgtca aggtcgccgg cacccgcatc
     6121 cgtcaccacc aagatcccga gctcgacgcg ctctttgcgt ccgccggcga gctggcccat
     6181 caccatctcc cgaccgccga tggcggtgag ctccatgtcg tcgaggccgg cgccggtcgg
     6241 cccgtcgtgc tgttgcacgg cgtgacgctc cagtggtggg tgtggtcggc gctgatctcg
     6301 ttgttgcgtg accggtatcg cgtggttgcg tgggacatgc gcggacacgg tgagtcgcgc
     6361 gccgggcgcg acggagtatc gctcgaggcc acggcgtcgg acctcgccat gctccttcgc
     6421 gaactggatc tcgacgacgc gatcctcgtg ggccactcca tgggcggcat gaccctcggg
     6481 cacttcgttg gacggcacag cgacgaactg gctgcccggg tgagtgcgct gtgcttcgtc
     6541 gccacgagcg gctgcgccac cgacaagtcg ttcagccgag gcaacctcaa gacgctgtac
     6601 gggctcgccg gccgagcgtt gctcgccggg ctgcgccggc cgtcgccgcg gtacccgtgg
     6661 cacgacaacg acctctccgc ggtgctcctc cgcaccgcgt tcggcccgtc ggccaccgcc
     6721 gacatgatcg acgccgtgcg ccggatgtcg gccgacatgg cgaccgcgtc gatgatcgaa
     6781 gcgggcaacg ctctcgccgc ccacgacgtg cgacaggtgc tgccgttcgt cgacatgccg
     6841 acgaccgtcg tggtcggcga cgccgaccgc atcacgccgc ccggccacgc ccgcgaactg
     6901 gccgagctga tccccggcgc gaccctcgtc gagctcgagg gtgtgggcca ccagatcatg
     6961 caggagtccc cggtcgccct ggccggcatc atcgacgatc tcgacggtcg ggcaccgagc
     7021 ctggtgcccg tcgcctgatc agccgatcgc ctcgctgacc agcgctgcat aggcgagccg
     7081 cccggccggc gtcaggtgca ccccgtcggc gacgaaccat tcgggatgtc cgtcggagcg
     7141 tgacttccag tcggcgaggc cgacgcccgg tgcccgggcg acggccgcag cgatccgttc
     7201 gttggcgagg tgctcccacg ggcgcggcgc cgccacgtcg acgaggacga gccggcggtc
     7261 ggccgtgagc gcaacgaggt cgtcgagctc ggtctcggtg cagggcccgt tcgtgcccat
     7321 gtgcacgacg acggtctgcg gcagcgagcc gtcggcgagg gatgcccgga cgatgcggac
     7381 ggcctcgcca cacgctcgcg agatcttggc gttcacggtg agcgaaccga tcagtgtgcg
     7441 catcgggtcg accgcgccgg ccatgacgga gtcaccgacg gcgaggaccc ccaccgggat
     7501 cgtggttgtg gtgggcgcag cggtcgtcgg cggggtgatc ggggcggtcg ggtcggccgt
     7561 cgtcgacacc acagcggtcg tcggcgcggc ggtcggtgcc acgtcgatga cctcggtcgg
     7621 ttggtccgcc agcccggagt cggcgtcggg aagatcgggc caggcggtga tcacggcgct
     7681 ggtcgcgagg gcggcggacg cgaagccgac gaaccagcgg gcccggagtc gctcctgggg
     7741 ggacgacggg cgacggtagg caccgacgac ccggccgagt gcaccgctgc ggaacggcgc
     7801 ttcgacgaag cggtacgaga gctcggcgag tgcgaacgac agcaccaacc ggaggatcag
     7861 caacgccgtg ccgtggatgt cgacgtcgag gtcggggcgg gtgaggacga tcaccggcca
     7921 gtgccacagg tagaggctgt aggagcgctg cccgatccac gccaacggac ggatgccgag
     7981 catcccgacc aggcgtccgg caggatgcac cgccgccgcg atcagcacca tcgtcgcgag
     8041 gtcgacgagc aggaatccgc ctcggtacac gaacgggtcg aactcgttca cccacaggaa
     8101 cgtgccgacg aggacgacga gcgcggcccc gccggtgatg tcgagcgttc gctgcgccgc
     8161 ggccggcaca cgggtcgggc gaaagcgctg cgccggccag atcgccgcga gggcaacacc
     8221 gacgagaagg cccgacgctc gggtgtcggt gccgaagaac acccgcgacg ggtccgccga
     8281 cgggtggtag agcgcggcca tgagcagcgc cgacgccacc gccccgagcc cggcaccgac
     8341 gaacaggtgg cggcgccgca ggcgtgcacc ggcgacggcc atgacgaacg ggaacacggc
     8401 gtagaactgc acctccacgg cgagcgacca caggtggcgc agcagcggcg gtcggccggc
     8461 ggcgaggaag tacgactcgt cgtggaggat ctgcgtccag ttcgacacgt agaggacggc
     8521 gcccggcaga tccgtccgga gctgtcgcag cgcgtcgggt gcgaacaccg cggcggcggc
     8581 gatgcagccc agcaccagca cggcaactgc cgggaccagc cgccggaccc ggccgagcca
     8641 gaagcggcgc agcgcgatcc cgccgctgac gtcgcgttgg tggagcagca acgatgcgat
     8701 caggtatccg ctgatcacca ggaacacctc gacgccgagg aacccgccgc cgaccaggac
     8761 gacatcggcg tggtagagca ccacggccag cacggcgagc gcccggaggc cgtcgagcgc
     8821 ggggaggtgc gcgaactctc gcggcacggg aggacgtgct cggcggcgcc gtcccggcga
     8881 gggcggtgcg ctcgaggggg gatcggcgac gccggtcatg gaaggttcga caccagttct
     8941 gtcgtgtgcg gtacggctgg tcccacgttc ccacaccggc acccgcgcgg ctcggatgtc
     9001 ccccgtgccc gcctccgggc ggccgggaac ctaccggcga gggtcggcga cgtggagcag
     9061 gccccgtgac tcgctgaggt ggtaggtgct catcgtccgt tcgtcgtgct gcatccgcca
     9121 ccggacgagg cgacggaggc agtccgcgac ctgaggtgtg tgggccgggt cgtcggcgag
     9181 gttgaccagt tggtgtgggt cgttgacgag gtcgaagagc agcggcggca tggcctcggc
     9241 ggcgccggcg aactgcacgt acttccattc gtcggtgcgg acgacgttga gcgagcagtg
     9301 ctcggccgga gcgttcagca ggaactcgct gaatcgatgc tcggggtcgt tgaacgccca
     9361 ctcccagaag acggcgtcgc gccagtgagg gggtgcgacg cccgcgtgca cgaaccgggt
     9421 gagcgggcgt ccgtcgacct ggtcgggaat cggaaccccg atccactcga ggatcgtggg
     9481 caggacgtcg accgactccg tgaactcgcc gacctgcagg ccgcgggtgg cgtcggcgtc
     9541 gggtcgggga tcgcggacga tcagggggat gtggtacgac tcgtcccagt agccgagctt
     9601 ctcggtgagc cagtggtcgc cgcccatctc accgtggtcg ctggtgatga cgacgagggt
     9661 gttgcggtcg aggccccgct cgcggaggtg tccgaagagc cgaccgagct ggtcgtccac
     9721 ctcggtctgc atgccgtggt acgtggcccg gagctggcgg cgatcgaggt cgtcgacagg
     9781 ggcaccgacc ccggccaacg ggatcgagac ggcgttgagc gggtgggcgc cgatctcgtc
     9841 gtcacggctg gcgtgtccga cgaagggcgg cacgtcgtcg gcggagtaca ggtcgtggta
     9901 cccgaccggg ttgcggtatg gcgggtgggg acggatgaag gaggcgtgca cgaagaacgg
     9961 ttctgcatcc cattcggcgg tcgtacggcc gtcgatccag tcgatgagtt cgctcaccat
    10021 gaacgcggtc tcggtgtgct cggcggcgaa gtgcgtgggc gcccaggtgg cgccgtgttc
    10081 ggccgagccg gggaagtcgg ggatcggcca gtagagctct cgggggttcg ccggaacgtc
    10141 gacgccctgg cggcgcagcc agtcggccca caccagcgag ccgtacacct cggggtcgtt
    10201 cacgatgggg tcgaagccgg gcaggacccc ctcgtagttg gagagacggg gatcgtccgg
    10261 cccgaccgtg cgggggtcga cgctcgtgtc ggtgtagccg aacagcgccg gggagtaacc
    10321 ggcctcacgg gcgagcagcg cgacgttggt gaagcgggcg tcgagcgggg tgccgttcag
    10381 gatcgaccgg tggttctggg cgtacatgcc ggtgtagagc gacgcccgac tcgggccgca
    10441 cggcgccgtg ttggcgaagt ggcgggcgaa caacacaccg tcggcggcga gcgcgtcgag
    10501 gttcggcgtc cggaccagcg gatgaccgac acaggacagc gagtcgccgc gccactggtc
    10561 gaccgtgatg aacaggacgt tgccggggcg ctggccggag ttcgtggagg gaacggtcat
    10621 gacggggaga ccgtagtgcc cggcgcgatc atgggacatg gtcatggatc gtgacagggg
    10681 gctcgccgac ctcggcggcc ggaccgacct cgccggcctg atggccgacc acgtcgtgcg
    10741 actcgacggt ggactgtcga cggccctcga ggagcgcggc cacctgctca ccgactccct
    10801 ctggacggcg cgtctgctgc tggaggcacc ggcggaggtg accgccgcgc atgcggcgtt
    10861 cgtggcggcc ggtgccgagg tgttgatcac ggccagctac caggtgtcgg agcaggggtt
    10921 catggccgcc ggcctcagcg ccgccgacgc gtccaacgcg ctccgggcga gcgtgggcgc
    10981 cgctcgggcg gccggcggcg cgctcgtggc cgcgtcggtc ggcccctacg gggcgatcct
    11041 ccacgatggt tcggagtacc ggggtcgcta cgggttgacg gtcgacgagc tggtcgcgtt
    11101 ccaccggcca cgcctcgacg tgctgctgtc ggcgaggccc gacgccatcg ccgtcgagac
    11161 gatcccggac ctcgacgagg cggctgcatt ggccgagctg ctcggagcgt tgccggcggg
    11221 cggtccaccg gcgtggatcg cgttctcgtg cgctgccgac gggcgcctgc actcgggcca
    11281 cgacgtcgcc gatgcggcgg ccctggtcgc tgcggtgccc ggcgtggtgg ccgtcggagt
    11341 gaactgcacc cgcccagccg acgtggcgac ggcgctcgag cgcatccggt cggcgacgga
    11401 gttgccgctg gtggtctacc cgaacggcgg tgggagctgg gacgcggagc acaactgttg
    11461 gcacgaccag gagttcgctg ccgacgacct ggtcgccgag tggctcgacc tcggggtccg
    11521 gctcctcggc gggtgctgcg gctacgggcc gtcggcgctg gccgcgttgg ggtcggtggt
    11581 ggcggcgctc gagcgttcgt agag
//
LOCUS       DAOSOA010000071        11480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5616_length_11480_cov_6.274748, whole genome shotgun sequence.
ACCESSION   DAOSOA010000071 DAOSOA010000000
VERSION     DAOSOA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11480
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5616_length_11480_cov_6.274748"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="PKY13_06450"
     CDS             <1..1189
                     /locus_tag="PKY13_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT79609.1"
                     /translation="VRFVPCARDTWRAPWSMYADLRDHDPVHRVTHADPGRDYWVLSR
                     FDDVFAAARDTATFSSAQGLTFEYDERNKAGLAEAAPMVMMDPPEHTAFRRLVGKGFT
                     PRHVQELEPAVRAFAVERIERLRSAGGGDVVAELFKPLPSFVVAHYLGVPPADRSRFD
                     GWTEGIVAANAQGDAMSAGDVVAELFLYFSDLIEQKRAHPGDDTISALVATGEAEVSI
                     LQVLGFAFTMVTGGNDTTTGLLGTGAELLTLNPGERAQLLAEPRLLDNAVEEMLRLAA
                     PVQGLARTTTRDVMVGDREIPAGRKVMLLYGSANRDPRRFGPTADEFDIDREVGTIMS
                     FGYGAHHCLGAAAARLQSRVVLEELLARCPRFAVDHEAGRFAPGHFVRRYESLPFVAD
                     TGA"
     gene            1186..1707
                     /locus_tag="PKY13_06455"
     CDS             1186..1707
                     /locus_tag="PKY13_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT79610.1"
                     /translation="MSDRILDAAGRLFAERGVAAVGMAEIAEAAGCSRATVYRAYENR
                     HVLRLAFVQREARRLGVAVAAKVRRIDDPARQLEVAVLSALSAVRSDPLLAAWFAEES
                     AGLAGGVAAASEVIDTLAASFLGDAGDRRTRDAARWVVRVVVSLLTVPGVDAAEERRL
                     VRQFLVPSVLSST"
     gene            1730..2734
                     /locus_tag="PKY13_06460"
     CDS             1730..2734
                     /locus_tag="PKY13_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79611.1"
                     /translation="MSDTKPRRTGVGVIAFPAAVVSVAWCLAVTRWSFEWPTALAALA
                     AALLPAVVAFVTTTELVAWRWARDRGVDFESALVPQVHRHLVRTRVARTFGVTVPMAV
                     DMMLMSRYNASDGSFRWFMDVWNPELRGLWQSGAGYVVATVWVELTKPRELTGRGPGV
                     AVLAPRRLRDFADATVIRWVAPMFVAVPIAASIWLAAPDPAVPVRNRLHEGVVVGALL
                     TAVLAVVVAAAVCRRSERAADDVAVAYEELTRSGTVNALLGVAIGMLGQVVGELLGSQ
                     PVAEWLTPWVTFPAGMLFGLGPLAIWYAAGTKFVFRSRRIDALRDRALAPGGVGAASA
                     "
     gene            2731..3105
                     /locus_tag="PKY13_06465"
     CDS             2731..3105
                     /locus_tag="PKY13_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT79612.1"
                     /translation="MIVRLDLASAASPATQIATQIAAGIDAGEVGPGAPLPTIRALAG
                     DLGVAPGTVARAYAELERDGWVVTQGRRGTSVAPGRPKRFDDDVAAAARALARAVRAS
                     RLDVGDAHRAVDVAFARLERGT"
     gene            3166..3966
                     /locus_tag="PKY13_06470"
     CDS             3166..3966
                     /locus_tag="PKY13_06470"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HOT79613.1"
                     /translation="MSRGTEAEAGSEATEPERRWPGQSRARFAELHRSGCFVIPNPWD
                     VGSARLLEGLGFQALATTSAGLAQSLGKRDQEVTLDELTRHVDALVSAVAIPVHVDSE
                     QCFADTPEGVAATVERLASVGASGLSIEDYSPVAGAIEPIEVFLPRVQAAAEAAHRHG
                     MLLTARAENALYGAGDLADSIARLQRYRDAGADVVYAPGLSDLADIGALVDAVGVPVN
                     VLLWGDAFTPEAAAAVGVRRLSTGSALAGAAFEAASLAASVLPSGRLG"
     gene            4052..5272
                     /locus_tag="PKY13_06475"
     CDS             4052..5272
                     /locus_tag="PKY13_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT79614.1"
                     /translation="MDFEFSEEQQAYKAEVIAFCDEHDDIDIFDVTRENMAQIVDTPK
                     RRAFMKEIGHKGWLGMTWPKEYGGSEGEGVYEYILNEELAGRGGPQIGKGVGIIGKTV
                     IAHGSDFLKEKFLPMILENDVEFAVGYSEPNAGSDAASMRLKGVKHTTEDGQEGWLLN
                     GQKTWTTSAHFAEWYWCGVRTDPDNKHMGITLMLIPMDHPGITINGIWTMGDERTNEV
                     WLDNVFVPDEYVVGEVNHGFKYISQALDLERFTMFTYSPIKQRLDVLTEYVQETTRDG
                     EPLKDDPIVRSKMANLHTEAEVARVMGLKFVAEAVKVEHLVREGKPYQPPTVVASEYK
                     LFATELSKRLASASMDIGGPGSQLRVKTEDAPMAGRSESTYRYTVIDTIGGGASEIQK
                     NIIARRGLGLPKNF"
     gene            complement(5312..5614)
                     /locus_tag="PKY13_06480"
     CDS             complement(5312..5614)
                     /locus_tag="PKY13_06480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT79615.1"
                     /translation="MDGVLAAYVIALIDGTTYRVLDGRMNTDFSAYSPGRILESAVLE
                     RALEDGETELFDWMTGVAPETILAANVWQPRWSLVAGAEPDSSAEADDQRPVAASV"
     gene            5739..6908
                     /locus_tag="PKY13_06485"
     CDS             5739..6908
                     /locus_tag="PKY13_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOT79616.1"
                     /translation="MTQIPALAGVRVLDLASVGPAARASRALADYGAEVIKLGAVPQA
                     GAVQIVPPFYAYSGNRGMQRALLDLKADAGREAFLKLAEGADVIIESFRPGVVDRLGI
                     GFAAVAERNPRIVYCSTSGFGQTGPRSSWAGHDVNYLATGGFLDCTGRRADGRPALPG
                     ATIADIAAGGMQATMAIMAGLLQRERTGVGEYLDVSIADGAFALMSLYVDEYLATGVE
                     PGPGHYILTGRYACYEVYECGDGKFISVGAIEPQFWRNLCGLLGLDEYATKQLDDDAQ
                     PEIAAAMTKVFRTKSRDEWVALLGPADTCVAPVNTVAEAVVDEQYVARGNVADAVHVT
                     EGPFRQSAPVWAGTVPPDGPYEIRDGSATDTEALLAAAGYAPHAIAELVESGVVA"
     gene            complement(6930..7751)
                     /locus_tag="PKY13_06490"
     CDS             complement(6930..7751)
                     /locus_tag="PKY13_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT79617.1"
                     /translation="MSDDQRAAVQQVFDGAADTYDAVGVSMFQPIAEQLVTDLSPRSG
                     ERALDVGCGRGAVLFRLAEAVGPTGRAIGIDLSPRMVEATAAEAAAAGLPAEVAVGDA
                     EAPDVPDAAFELVTSSLVLFFLPDPAAALVAWHRKLVEGGRLGVSTFGPLSDSWYDVD
                     ATFAPHLPPGMADPRTRQADTPFGSDEGMERLVADAGFSDVRTVLTTVPVRFDDEEQW
                     YRWTWSTGQRRMWAAVPEAERPTVRAEAEARLQATRDRDGRIGFDQVARFTYGRR"
     assembly_gap    7894..7993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8001..8531
                     /locus_tag="PKY13_06495"
     CDS             8001..8531
                     /locus_tag="PKY13_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOT79618.1"
                     /translation="MADETAVSDLPSEVTDMIGVPLYHQSADFDVERGYTYNTCAATQ
                     NANPVYWDDSVAAEITGGPIAPPTTISLWMRPHFWQPGAEGEQVALQVHFDLKRIFGL
                     PEAVMTDNTITFYDPVRPGDRISNRQVLTSVSGPKTTKLGTGRFWVIDVEFHNQRDEL
                     VGIETYTGFGYVRGAA"
     gene            8528..9022
                     /locus_tag="PKY13_06500"
     CDS             8528..9022
                     /locus_tag="PKY13_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOT79619.1"
                     /translation="MTTKLTLDQVTVGDAVPALAVPVTPTTVVLGAIASRDWRPMHHD
                     YKFATERNGVQDIFLNTPNQAAWFERFVTDWTGPTGRLGRMKFRMRDSIFPGDTMTFT
                     ASVTDVTTDETGCGWAGLEVTLSATSAERPEPRVCSTCSVRVAVPTSADDNPWSRRGD
                     QWKP"
     gene            9067..10206
                     /locus_tag="PKY13_06505"
     CDS             9067..10206
                     /locus_tag="PKY13_06505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT79620.1"
                     /translation="MLNLDFTEEQDMLREMVRGVCEQYANLDVVRAMEDDAVGYPVDL
                     WAQLAELGILGMTIPEEHGGSGMTMQEGVVVYEELGRALAPLPHLVSAVVSAGAILTG
                     GSAEQIAEWVPKLASGEAIFTPAWTEPDNGFGPKGVQVEAVADGDGFTITGTKQHVYF
                     ASSAQRLIVLARTGAEPAAIDLFLVDPAADGVTLTQKMSISSDTQYRVDFAGVRVSAA
                     DRIGPAGSGWATWQTVMNDAMILQAAYANGGNEHALDITVQYSKDREQFDKPLGAFQA
                     LSHDMADAKTALDGSKVLNYEAAWAHSLGRPVTKLAAMSKLFACNSYRDTTAMAQQIF
                     GGVGFTLEYEIQLYFRRAKQQQITWNDTRTCEDLIAAAVLDESAA"
     gene            10251..10622
                     /locus_tag="PKY13_06510"
     CDS             10251..10622
                     /locus_tag="PKY13_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79621.1"
                     /translation="MPVVLIVALLLVVVGLGIAAVRVQRRGVPEPTSPSERAAHWSRI
                     VSGALLVVAAILVVVGIVAGLEVWPPSLDTYPGLESLDTSGVDIVPLASYLIWAVTGG
                     LAAALFGISWAIRSIERVRGT"
     gene            complement(10579..11193)
                     /locus_tag="PKY13_06515"
     CDS             complement(10579..11193)
                     /locus_tag="PKY13_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79622.1"
                     /translation="MGAVISRRRFVLFGLGVGSTVGAFAVPAGAAATPSPTTATLWRL
                     SADWGYPAGPKGRTRCKGRACHLHAANKIYATEADALAGRLHICCMAQPVAFEVPIEV
                     HHELTSLPPSPGPALGDRRHDEVVQALERAALAAHESSHHAAPPSRPPATLASTGSSV
                     AMLTAGGVALGTLGATALAISRRNEATDDQLRCPGPARSTGSPS"
BASE COUNT         1698 a         3930 c         3981 g         1771 t
ORIGIN      
        1 ggtgaggttc gtgccgtgtg cccgcgacac ctggcgagcg ccgtggtcca tgtacgccga
       61 cctccgcgac cacgaccccg ttcaccgcgt cacccacgcc gatcccgggc gcgactactg
      121 ggtgctgtcg cgcttcgacg acgtcttcgc tgcggcgcga gacaccgcca cgttctcgtc
      181 ggcgcaggga ctgacgttcg agtacgacga gcggaacaag gcggggctcg ccgaggccgc
      241 tccgatggtc atgatggatc cgccggaaca cactgcgttc cgtcgcctgg tgggcaaggg
      301 gttcacgccg cggcacgtac aggagctcga gccggcggtg cgggccttcg cggtggaacg
      361 gatcgagcgt ctccgttcgg ccggtggcgg cgacgtcgtg gccgagttgt tcaagccgct
      421 ccccagcttc gtggtcgccc actacctggg agtgccgccc gccgatcgat cgcgattcga
      481 cgggtggacc gagggcatcg tggccgccaa cgcccagggt gacgcgatgt cggccggcga
      541 cgtggtcgcg gagttgttcc tgtacttcag tgacctgatc gagcagaagc gtgctcaccc
      601 cggcgacgac acgatctccg cactcgtcgc gacgggcgag gccgaggtct ccatcctgca
      661 ggtgctgggg ttcgccttca cgatggtgac cggcggcaac gacaccacga ccggcctcct
      721 cggcaccggc gccgaactgt tgacgctcaa ccctggtgag cgggcgcagc tgctcgccga
      781 accacggctc ctcgacaacg ccgtcgagga gatgcttcga ctggcagcac ctgtgcaggg
      841 gctcgctcgc actacgaccc gggacgtgat ggtgggagat cgcgagatcc ccgcgggccg
      901 caaggtgatg ctcctctacg gctcggccaa tcgcgacccg cgacgattcg ggccaacggc
      961 cgacgagttc gacatcgacc gtgaggtcgg cacgatcatg agtttcgggt acggcgctca
     1021 ccactgcctc ggcgccgccg cggcgcggct ccagtcccgg gttgtgctcg aggaactgct
     1081 ggcccggtgc cctcggttcg cggtcgacca cgaggccggt cgattcgccc cgggccactt
     1141 cgtgcgtcgc tacgagtcgc tgccgttcgt ggcggacacc ggcgcgtgag cgatcggatc
     1201 ctcgacgccg ccggtcgcct cttcgccgaa cggggagtgg ccgccgtcgg catggccgag
     1261 atcgccgagg ccgccggatg ctcacgggcg accgtctatc gggcctacga gaaccgccac
     1321 gtgctgcgac tcgccttcgt gcagcgcgag gcccggcggc tgggagtggc ggtggcggcg
     1381 aaggtccggc gcatcgacga ccctgccagg cagctggagg tcgcggtgct gtcggcgctg
     1441 tccgccgtgc gatccgaccc gttgctggcc gcctggttcg cggaggagtc cgccggtctg
     1501 gccggcggcg tcgcggcggc gtcggaggtg atcgacaccc tggcagcgtc cttcctcggc
     1561 gacgccgggg atcgtcggac ccgcgacgcc gccaggtggg tggtccgggt cgtcgtctcg
     1621 ctcctgacgg tgccgggtgt cgatgcggcc gaggagcgtc ggctggtccg ccagttcctg
     1681 gtgccatcgg tgttgtcgag cacctgacgt ctgtgtacgt tgtatcggca tgagcgatac
     1741 aaagccgcgg cgaacgggag tcggtgtcat cgccttcccg gcggcggtcg tgtcggttgc
     1801 atggtgcctg gccgtgaccc gatggtcctt cgagtggccg acagccctcg ctgcgctcgc
     1861 tgcggcgctt cttcctgcgg tcgtggcctt cgtcaccacg acggagctcg tggcgtggcg
     1921 atgggctcgg gaccggggcg tggacttcga gtcggcgctg gtgccgcagg ttcaccgcca
     1981 cctcgtccgg acgcgggtgg cgcggacgtt cggggtgacc gtgccgatgg ccgtcgacat
     2041 gatgctgatg tcgcggtaca acgcgtccga cggctcgttc cggtggttca tggacgtttg
     2101 gaaccccgag ctccggggcc tgtggcagtc cggcgcgggg tacgtggttg cgaccgtctg
     2161 ggtcgagctg accaagccgc gggaactgac gggtcgtggc ccgggtgtgg ccgtgctcgc
     2221 accacggcgg ctgcgcgact tcgccgacgc gacggtgatc cggtgggttg ccccgatgtt
     2281 cgtcgccgtg cccatcgcgg cgtcgatctg gttggcggca cccgatcccg ccgtcccggt
     2341 ccggaaccgc ttgcacgaag gggtggtggt cggggcactc ctgaccgccg tgctcgctgt
     2401 ggtggtcgcc gctgcggtgt gccgtcgatc cgagcgcgcg gcggacgacg tcgcagtcgc
     2461 gtacgaggag ctgacgcgct ccggcacggt caacgcactc ctcggggtgg ccatcgggat
     2521 gctcggccag gtcgtcgggg agctgctcgg gtcccagccc gttgccgagt ggctcacacc
     2581 gtgggtcacg ttccccgcag ggatgctctt cgggctcggc ccgctggcga tctggtacgc
     2641 cgccgggacg aagttcgtgt tccggagccg gcgcatcgac gcgctgcgcg accgggcgct
     2701 cgcgcccggg ggcgtcgggg cggcgtcggc atgatcgtcc ggctcgacct cgcgtctgcg
     2761 gcatcgccgg cgacgcagat cgcgacccag atcgccgccg gcatcgacgc cggcgaggtc
     2821 ggtccgggcg caccgctccc gacgatccgg gcgctcgccg gagacctcgg agtcgcgccc
     2881 ggcaccgtcg cccgcgccta cgccgaactc gagcgcgacg ggtgggtcgt gacacagggg
     2941 cgacggggga cgtcggtcgc ccccggccgt cccaagcggt tcgacgacga cgtcgccgcg
     3001 gcggcgcgcg ccctcgcccg cgccgtcagg gcgtcccggc tcgacgtcgg cgacgcccac
     3061 cgagcggtcg acgtcgcctt cgcccgcctc gaacgcggga cctgacggga ccgccccgac
     3121 cgattcctgc gactcgctgt cacgggcggg cgcgaggatg ggcgcatgag ccgaggtacc
     3181 gaagcggaag cagggagcga agcgaccgag cctgagcgga ggtggccggg gcaatcgaga
     3241 gcgcgcttcg ccgaactgca ccgcagcggg tgcttcgtca tccccaaccc gtgggatgtc
     3301 gggtcggccc gtctcctcga ggggctgggg ttccaggccc ttgcgaccac gagcgcaggg
     3361 ctggcgcagt cgctcggcaa gcgcgaccag gaggtcacgc tcgacgagct gacgcgccac
     3421 gtcgacgcgt tggtctcggc cgtggcgatc ccggtccacg tcgactccga gcagtgcttc
     3481 gcggacacac cggagggggt ggcggcgacc gtcgagcggt tggcctcggt gggtgcctcc
     3541 ggtctgtcga tcgaggacta ctcacctgtc gccggagcga tcgagccgat cgaggtcttc
     3601 ctgccgcggg tgcaggccgc cgccgaggcc gcgcaccgtc acggcatgct cctcactgcc
     3661 cgcgccgaga acgcgctcta cggcgccggc gacctcgccg actcgatcgc tcgcctgcag
     3721 aggtaccggg acgccggcgc cgatgtcgtc tatgcgcccg ggttgtccga tctcgccgac
     3781 atcggtgccc tggtcgatgc cgtcggcgta ccggtcaacg tgctgttgtg gggcgatgcc
     3841 ttcaccccgg aggcggccgc tgctgtcggc gtccgccggc tgtcgaccgg aagcgcactg
     3901 gcgggcgccg cgttcgaggc ggcgagcctc gctgcgagtg tgttgccgtc ggggcggttg
     3961 ggctgagccc gattgcaggt cgcgagagcg gggtcgtaga atctgacgga ccgtcagaaa
     4021 ccggcggccg gacgagggtg ggagcgacgc catggatttc gagttctccg aggagcagca
     4081 ggcttacaag gccgaggtca tcgccttctg cgacgagcac gacgacatcg acatcttcga
     4141 cgtgacccgc gagaacatgg cgcagatcgt cgacacgccc aagcggcggg cgttcatgaa
     4201 ggagatcggc cacaagggct ggctcggcat gacctggccc aaggagtacg gcggcagcga
     4261 gggtgagggc gtctacgagt acatcctcaa cgaggagctc gccggtcgcg gcggtcccca
     4321 gatcggcaag ggcgtcggca tcatcggcaa gacggtcatc gcccacggct cggacttcct
     4381 caaggagaag ttcctgccga tgatcctcga gaacgacgtc gagttcgccg tcggctactc
     4441 cgagcccaac gccggttccg atgccgcctc gatgcgcctc aagggtgtca agcacacgac
     4501 cgaggacggc caggagggct ggctgctcaa cggccagaag acgtggacga cgtcggccca
     4561 cttcgccgag tggtactggt gcggcgtccg caccgatccc gacaacaagc acatgggcat
     4621 caccctcatg ctcatcccga tggaccaccc gggcatcacg atcaacggca tctggaccat
     4681 gggcgacgag cgcaccaacg aggtgtggct cgacaacgtg ttcgtccccg acgagtacgt
     4741 cgtcggcgag gtcaaccacg ggttcaagta catctcccag gcgctcgacc tcgagcgctt
     4801 caccatgttc acctactcgc cgatcaagca gcgtctcgat gtgctgaccg agtacgtcca
     4861 ggaaacgacc cgcgacggtg aaccgctgaa ggacgacccg atcgtccgtt cgaagatggc
     4921 caacctccac accgaggccg aggtcgctcg tgtgatgggt ctcaagttcg tcgccgaagc
     4981 ggtgaaggtc gagcacctcg tccgcgaggg caagccgtac cagccgccga ccgtcgtggc
     5041 atcggagtac aagctgttcg cgaccgagct ctccaagcgc ctcgcgagtg cgtcgatgga
     5101 catcggtggc cccggcagcc agctccgggt gaagaccgag gacgctccga tggccggccg
     5161 ctcggagtcg acctaccgct acaccgtgat cgacaccatc ggtggcggtg catcggagat
     5221 ccagaagaac atcatcgccc gccgcgggct ggggctcccc aagaacttct gaccccggcg
     5281 tcgctgtgtc ggggcgctcc gcccgacgtt gtcagaccga cgcggcgacc ggacgctggt
     5341 cgtccgcttc ggcggacgag tccggttcgg cgccggcgac gagcgaccat cgcggctgcc
     5401 acacgttggc cgcgaggatc gtctcggggg cgacgccggt catccagtcg aagagttcgg
     5461 tctcaccgtc ctcgagcgcc cgttccagaa ccgccgattc gaggatgcgg ccgggtgagt
     5521 acgcgctgaa gtccgtgttc atccgaccgt cgaggacccg gtacgtggtc ccgtcgatca
     5581 acgcgatcac gtacgcggcg aggacgccgt cgatcgacgg agatctctag ttcgtcggaa
     5641 gcgcagtttc gttcggattc gggggcagaa tgagtcggac ggcccagtgc cggagggtca
     5701 agctgactga cccgccgaag ctgtgtcagg gtggcggcat gacgcagatt ccagccctcg
     5761 ccggtgttcg cgtgctcgac ctcgcctcgg tcgggcccgc ggcgcgcgcc tcccgtgccc
     5821 tcgccgacta cggcgccgag gtgatcaagc tcggcgccgt tcctcaagcc ggcgccgtgc
     5881 agatcgtgcc gccgttctac gcctattcgg gcaatcgggg catgcaacgg gcgctcctcg
     5941 atctcaaggc cgacgccgga cgcgaagcgt tcctgaagtt ggccgagggc gccgatgtga
     6001 tcatcgagtc gttccgtccc ggggtcgtcg atcgcctcgg catcgggttc gcagcggtgg
     6061 cggagcggaa cccgcggatc gtgtactgct cgaccagcgg cttcggccag accggccccc
     6121 ggagttcgtg ggccggccac gacgtgaact acctggccac tgggggcttc ctcgactgca
     6181 ccggtcgccg cgccgacggc cgcccggccc ttccgggcgc gacgatcgcg gacatcgcgg
     6241 cgggcggtat gcaggcgacg atggcgatca tggccggtct gctgcagcgg gagcggacgg
     6301 gcgtgggcga gtacctcgac gtgtcgatcg ccgacggtgc cttcgccctg atgtcgctct
     6361 acgtcgacga gtacctcgcc accggcgtcg agcccgggcc gggccattac atcctcaccg
     6421 gtcgctacgc ctgctacgag gtctacgagt gcggcgacgg caagttcatc tccgtcgggg
     6481 cgatcgagcc gcagttctgg cgcaatctgt gtggactgct cggcctcgac gagtacgcga
     6541 ccaaacaact cgacgacgac gcccagcccg agatcgccgc ggcgatgacc aaggtgttcc
     6601 gcacgaagtc gcgtgacgag tgggtcgcgc tcctggggcc ggcggacacc tgcgtcgctc
     6661 cggtgaacac ggtcgccgag gcggtcgtcg atgagcagta cgtggcccgg ggcaacgtcg
     6721 ccgacgccgt gcatgtgacc gaggggccgt tccggcagtc ggccccggtg tgggcgggca
     6781 ccgtgccgcc cgatggtccc tacgagatcc gcgacgggtc cgccaccgac accgaggccc
     6841 tgctggctgc ggccggctac gccccgcacg cgatcgcgga gctggtggag tcgggcgtcg
     6901 tcgcgtgacg cgtcggtctc gatcgtcggt cagcggcggc cgtacgtgaa ccgtgccacc
     6961 tggtcgaagc cgatgcgccc gtcgcggtcg cgggtggcct gaaggcgcgc ctcggcctcg
     7021 gcgcgcaccg tcggccgctc cgcctccggg acggcggccc acatccgtcg ctggccggtc
     7081 gaccacgtcc accggtacca ctgctcttcg tcgtcgaacc gcaccggcac cgtggtgagg
     7141 accgtccgca cgtcgctgaa cccggcgtcg gcgaccagcc gctccatgcc ctcgtcggag
     7201 ccgaaggggg tgtcggcctg gcgagtgcgg ggatcggcca tccccggtgg gaggtgcggg
     7261 gcgaaggtgg cgtcgacgtc gtaccacgag tcgctcagcg gcccgaacgt cgacacgccg
     7321 aggcgtccac cctcgacgag cttgcggtgc cacgccacga gcgcggccgc cgggtcgggc
     7381 aggaagaaca gcaccagtga cgaggtcacg agctcgaacg ccgcgtcggg gacgtcgggc
     7441 gcctcagcat cgccgacggc cacctccgct ggcaggccgg ccgccgcggc ctcggccgct
     7501 gtcgcctcga ccatgcgcgg cgacaggtcg atgccgatgg cgcgcccggt cgggccgacg
     7561 gcttcggcga gacggaacag cacagcgccg cgaccgcatc cgacgtcgag ggctcgttcg
     7621 cccgaccggg gcgacaggtc cgtgaccaac tgctcggcga tcggttggaa catcgacacg
     7681 cccaccgcgt cgtacgtgtc ggccgcgccg tcgaacacct gctgcaccgc tgcccgctgg
     7741 tcgtcgctca tcgggacagc ttcgcgccga tttgggcgct tggggccgtg ctcgtagaat
     7801 ctgacggttc gtcagatttg gatggtggag ggggagaacg agcccatggc agacgagacc
     7861 gcggtgtccg acctcccgtc ggaggtcacc gacnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnncgagccc atggcagacg agaccgcggt gtccgacctc ccgtcggagg
     8041 tcaccgacat gatcggggtg ccgctctatc accagagcgc cgacttcgac gtcgagcggg
     8101 gctacaccta caacacgtgc gccgcgaccc agaacgccaa cccggtgtac tgggacgaca
     8161 gcgtcgccgc cgagatcacc ggcgggccga tcgctccccc caccaccatc tcgctgtgga
     8221 tgcgcccgca cttctggcag cccggcgccg agggcgagca ggttgccctg caggtgcact
     8281 tcgacctcaa gcgcatcttc ggactgcccg aggccgtgat gaccgacaac acgatcacgt
     8341 tctacgaccc ggtccgcccc ggggaccgca tcagcaatcg ccaggttctc acgtcggtct
     8401 cgggccccaa gacgaccaag ctcggcaccg gccggttctg ggtgatcgac gtcgagttcc
     8461 acaaccagcg cgacgagctc gtcggcatcg agacctacac cgggttcggc tacgtgcggg
     8521 gtgcggcatg accacgaagc tcaccctcga ccaggtcacc gtcggcgatg ccgtgccggc
     8581 gctcgccgtt ccggtcactc ccacgacggt cgtcctcggt gcaatcgcca gccgcgactg
     8641 gcggcccatg catcacgact acaagttcgc caccgaacgc aacggagtgc aggacatctt
     8701 cctgaacacg ccgaaccagg cggcctggtt cgaacgcttc gtgaccgact ggaccggtcc
     8761 gaccggccga ctcggccgca tgaagttccg catgcgcgat tccatcttcc ccggcgacac
     8821 gatgaccttc acggcgagcg tcaccgacgt gacgaccgac gagaccggct gcggctgggc
     8881 cggcctcgag gtcacgctgt cggcgacgag tgccgaacgt cccgagccgc gggtctgcag
     8941 cacgtgctcc gtgcgcgtcg ccgttcccac atcggccgac gacaacccct ggtcccgtcg
     9001 tggcgaccag tggaagccct gacccatccc gacctgaacc accaagcctg agagagcgag
     9061 aacccgatgc tgaacctcga cttcaccgaa gagcaggaca tgctccgcga gatggtgcgc
     9121 ggcgtatgcg agcagtacgc caacctcgac gtggtccggg ccatggagga cgacgccgtc
     9181 ggctatcccg tcgacctgtg ggcccagctc gcagagctgg gcatcctcgg catgaccatc
     9241 cccgaggagc acggcggctc cggcatgacg atgcaggaag gcgtggtcgt ctacgaggag
     9301 ctcggacgtg cgctcgcacc gctcccgcac ctcgtcagcg cggtcgtctc ggcgggggcg
     9361 atcctcaccg gcgggagcgc cgagcagatc gccgagtggg tgccgaagct tgcgtccggc
     9421 gaggccatct tcactccggc ctggaccgag cccgacaacg gcttcggacc caagggtgtg
     9481 caggtcgagg cggtcgccga cggcgacggg ttcacgatca ccggcaccaa gcagcacgtc
     9541 tacttcgcat cgtcggcaca gcggctgatc gtgctcgccc gcaccggtgc ggaaccggcg
     9601 gcgatcgatc tcttcctggt ggatcccgcc gctgatgggg tcacgctcac ccagaagatg
     9661 tcgatcagct ccgacaccca gtaccgcgtc gacttcgccg gagtgagggt ctcggcggcc
     9721 gaccgcatcg gtccggccgg gtccggctgg gccacgtggc agaccgtgat gaacgacgcc
     9781 atgatcctcc aggcggccta cgccaacggt ggcaacgagc acgccctcga catcaccgtg
     9841 cagtactcca aggaccgcga gcagttcgac aagccgcttg gcgcctttca ggcgctgtcg
     9901 cacgacatgg ccgacgccaa gacggccctc gacgggtcga aggtgctcaa ctacgaggcg
     9961 gcctgggccc actccctcgg ccggcccgtc accaagttgg cggccatgtc gaagctgttc
    10021 gcgtgcaaca gctaccgcga caccacggcg atggcccagc agatcttcgg tggcgtgggc
    10081 ttcacgctcg agtacgagat ccagctctac ttccgtcgtg ccaagcagca gcagatcacc
    10141 tggaacgaca cccgcacctg cgaagacctc atcgccgccg cggtcctgga cgagtccgcc
    10201 gcgtaggcgc cgagccggag gcgcttccaa aggcgcgata cgtttgtgat gtgcctgttg
    10261 tcctgatcgt tgccctgttg ctcgtcgtgg tgggtctcgg catcgctgcg gtccgggtgc
    10321 agcgccgagg tgtgccggag ccgacatctc catccgaacg cgcggcccac tggagccgga
    10381 ttgtctcggg agcgctcctg gttgttgccg cgatcctcgt ggtcgtcggc atcgtggcgg
    10441 gtctcgaggt ctggccgccg tcgctcgaca cctacccggg gctggaatcg ctcgacacgt
    10501 ccggcgtcga catcgtgccc ctcgcgtcgt acctgatctg ggcggtcacc ggtgggttgg
    10561 ccgctgccct gttcggaatc agctgggcga tccggtcgat cgagcgggtc cggggcacct
    10621 gagctggtcg tcggtcgcct cgttgcgccg cgagatcgcc agtgccgttg ctccgagagt
    10681 ccccagcgcg accccacctg ctgtcagcat cgcaacgctg gatccggtgc tcgcgagcgt
    10741 cgccggcggt cggctcggcg gcgccgcgtg atgggacgat tcgtgggctg cgagcgcggc
    10801 ccgttcgagg gcctggacga cctcgtcgtg acgccggtcg ccgagcgcgg ggcccggtga
    10861 cggcgggagg ctcgtcagct cgtggtgcac ctcgatcggg acctcgaagg cgaccggctg
    10921 ggccatgcag cagatgtgca ggcgccccgc caacgcatcc gcctcggtcg catagatctt
    10981 gttggcggcg tgaaggtggc acgcacggcc cttgcaacgg gttctgccct tcggcccagc
    11041 ggggtacccc cagtcggcgg agagccgcca gagcgtcgcc gtcgtggggg acggtgtggc
    11101 cgccgcccca gcagggacag cgaacgcccc caccgtcgaa ccgacgccga gcccgaagag
    11161 cacgaaccga cgtcgactga tcactgcacc cacaaccatt cccttcaaac gcgatgcgtc
    11221 ctcatcgtcg ccgtggcggg agcgattgaa acattgggca ggattgccca ccgaacaagg
    11281 ttcgggactg atccggacgg cccagaccac cgagacggcg gtccgctgcg ccgctcagtc
    11341 gagcagggcg atcagcttct tgggggcggt gaggcggtag ctgtcctcga cgatcgcagc
    11401 gagctcgtca cggtcgacgt ccacaaagtt cacgccgatc cacccattgc ggccgacgta
    11461 ggccggcacg tagaagcgct
//
LOCUS       DAOSOA010000072        11258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5751_length_11258_cov_6.354012, whole genome shotgun sequence.
ACCESSION   DAOSOA010000072 DAOSOA010000000
VERSION     DAOSOA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5751_length_11258_cov_6.354012"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKY13_06520"
     CDS             complement(<1..266)
                     /locus_tag="PKY13_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79623.1"
                     /translation="MLELVGDGWGRRIAVGERVTFGRGGDDVDIVVADDPRLHRCCGV
                     ITVDDHGWELVNTGRWLRLRVVSLDRFGVDSLFPGQRLHVPWSEA"
     gene            434..1468
                     /locus_tag="PKY13_06525"
     CDS             434..1468
                     /locus_tag="PKY13_06525"
                     /inference="COORDINATES: protein motif:HMM:NF018018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT79624.1"
                     /translation="MRLGSDVDRLDGLSLLLEDGAGLLVRSRSDLLWETAATVWTGPS
                     RQRVAHDLERTMALADRIAERLATLGREARAQSDRQRRASLAGRPTIDDARATLLFSD
                     LHGDGRAAMAIGDVATATHVVVLVPGMGSDASNFAALVADARRLQYHAGLRLGGSGSV
                     AVVAWLGYDAPAGLDDPLALGQVLGRDRANAGASALRTFLRELDRRDDSSVTLIGHSY
                     GSLVVAGAARANTEVDRVVVLGSPGLGVADVGGLGLSPGTALYAGAFDNDPVARSEWF
                     GRDPSSFGFGALPLATDPWPQDDPPSVLHAHSAYFDEDSQSLDNLARLVTGGVPRGSI
                     AVARRVTGTR"
     gene            complement(1452..>2178)
                     /locus_tag="PKY13_06530"
     CDS             complement(1452..>2178)
                     /locus_tag="PKY13_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT79625.1"
                     /translation="RVRDVGVAACVALGHAHARGVVHRDVKPANLLVSDSGEVALGDF
                     DIAAGGSLPSSTITKDSMSPPHAPPERLNGDGPSGPSSDVWSLGSTLYTLLDDRPPFG
                     TADSPGGMAGLIQRVLHDPLPPLRRTDVPSDLFAVLQRAMAKNPADRWPDVASMADGL
                     RHVELEPAVSSAPTRARSLARPEASVADPTEPPAPVAAPSTPPEPATSGSRWAGFTDA
                     ATIAIVVVAVLVAVAATVVIAFR"
     assembly_gap    2179..2278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2279..2559)
                     /locus_tag="PKY13_06535"
     CDS             complement(<2279..2559)
                     /locus_tag="PKY13_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HOT79626.1"
                     /translation="MSSDRTPTNTVAHDVAIVAARAADAKQADSVMVLDVGPIIGICD
                     YFVICSARNSRLVAAVAQEIEDHVAVEFDRRPMSVEGLDDKKWVLVDFG"
     gene            complement(2595..3872)
                     /locus_tag="PKY13_06540"
     CDS             complement(2595..3872)
                     /locus_tag="PKY13_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOT79627.1"
                     /translation="MSETMTEPRHPGGAGRGESGSSSSPRADGDAPVVHRAHAWKRRR
                     LAVRIFSVLTVLAIGGLGWLGVQGYDAARKIKGGATATIVTDPAAPGFVAAVDASPVH
                     LVAVTDDQDALSAFLVLVPDPAGGSGSIVWSLGELVIEVDGASTSLAEVYKSGGLDAA
                     RAEFEKVLGFGSTDATIVGPTELADAAAGLGTVSVKNPDPISVEVKGKKVEKFKAGTI
                     ELAPDELGEYLTLRGAGERPENRSTRAEALLTALQEQLGGSGETTTGGPTTAAPSVSE
                     TNDLGTLVTRMGTSSFDFIVLPTTRQSFKGSYLYSPDPEAITEQLNGVVQFPVSSFPG
                     QRPRVRVLNGTTDTTMASRIAPDLARAGGEVLLVGNAKSLDVGSTTVVYSNDEFKGVA
                     DAIAEAVGVSAERTDEISDAADIDVVLGADHQP"
     gene            complement(3869..4456)
                     /gene="nadD"
                     /locus_tag="PKY13_06545"
     CDS             complement(3869..4456)
                     /gene="nadD"
                     /locus_tag="PKY13_06545"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOT79628.1"
                     /translation="MTRRLAVFGGTFDPPHVGHLVVASEVRYRGGFDEVLLMVANDPW
                     QKHGTRDITPAAVRFEMVAAAVAGHDGLVASNLEIGRGGPTYTVDTVEQLTAGVDVDV
                     TVVLGADAVAGLDSWHRAEDLASMVSIVAVARPGHPAPSASPRWRLRVVDVPAIEVSS
                     TDLRSRCASGAPIDYLVPDAVRSVIEQRGLYGVRR"
     gene            4698..5528
                     /locus_tag="PKY13_06550"
     CDS             4698..5528
                     /locus_tag="PKY13_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT79629.1"
                     /translation="MSDSVGVYLNEIGKVPLLTAMEEIELSKAIEAGVEARARKEAGE
                     RGADINRKIAAANAAKDRFIRANLRLVVSVARRYPLPSGMELLDLIQEGNLGLEHAVD
                     KFDWRKGFKFSTYATFWIRQSIGRALDQKASLIRLPGDRSASLRAALRAVSGDGDELD
                     TENALLHRLATPTSLDRSVGHDDSNELVDLLADSAPGPEAEYMAQVDDELLDKVLSVL
                     DPRARSAVEQRFGLADGCRRSYREVGEELGVTAEAARRLVKRAVNSVRETALATIDAA
                     "
     gene            5579..6922
                     /locus_tag="PKY13_06555"
     CDS             5579..6922
                     /locus_tag="PKY13_06555"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HOT79630.1"
                     /translation="MTAEWNPSSWRERPAQQQPVWPDESATDAVLKTLNGYPPLVFAG
                     ESRNLLSELGRVAQGEAFLLQAGDCAESFDAFNATSIRERLKLILQMAVVLTYSAGVP
                     VVKVGRIAGQYAKPRSSNTELVDGVELPSFRGHIVNDPAPTASARIPDPNRLIDAYHR
                     SASTLNLLRAFTKGGFADMNRVHAWNQEFVASSAEGARYEALAGQIDRALRFMAACGI
                     DTTSVPRLHEVDFYTSHEALVLGYEEALTRQDSLTGDWYDCSAHMVWIGERTRGLDDA
                     HIEFFRGIHNPIGCKVGPTATVEEVLGLCEALNPERIPGRLTLISRMGADKVRDHLPP
                     ILSAVRDAGHPVVWACDPMHGNTFTSQGGRKTRHFDAILDEIRGFFEAHAGVGTWPGG
                     VHVELTSDDVTECLGGAERLGDSDLGLRYETICDPRLNGRQSLDLAFEVAELLRR"
     assembly_gap    7153..7252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7253..9087
                     /locus_tag="PKY13_06560"
     CDS             <7253..9087
                     /locus_tag="PKY13_06560"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HOT79631.1"
                     /translation="PVTTIEIDPDLAKRTQRGDIFEVPADMAAEIEADIAKFEKMLAL
                     HLAGEIEDDTFRVFRLNNGIYGQRQGGTNQMVRVKAPYGKLNAQQLDLFARVADEYSR
                     GWGHITTRQNFQFHYVELTRVPDLLKEFAALGMTTREACGDTVRNVQGCHLAGACPHE
                     KLDITAWAEAAHRHFLHHPFAQRLPRKFKINFSGCETDCGQAMFNDAGVIAVTRTLED
                     GTVEAGFRVFVGGGLGANPHAALALEEFTPRENLLPTIEAILRIFEQHGNRKNKLRAR
                     LKWVIQDLGWDEVQARILNSRRLLPASSTWPGGIPEVVQQWGDTPAGVAAGVTPTVIG
                     QGTPVALRSTKPYERWEQANVIRGSANGTVSAIAWSRLGDVTSDQFRGLASITREFNA
                     EVRITNRQNLVFRDLTEEQLPLLFERLTAIGMAEPGAELSRDVVACPGADTCNIAVTQ
                     SRGLADAIGVALEEAGLGEVGGIRTNISGCTNSCGQHHTSDIGFFGAERRAHGKAAPG
                     YQMLLGGYVGQEQVHFGEKALRLPAKAAPQAVVRVVGRFNDERQAGETFRSWIERVGG
                     VKEIAATLKDLDIFPTPEDGPDFYVDYDETGPFEVEIGDSECAV"
     gene            9087..>9484
                     /locus_tag="PKY13_06565"
     CDS             9087..>9484
                     /locus_tag="PKY13_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOT79632.1"
                     /translation="MSETSLSVPSARFAPAEFSDEELADLNSDFERLPASKIIQWAVD
                     SFGPYLSLAASMADALVIDIATRVDPAIEVVFIDTGYHFPETLETVETIRRHYGLNLR
                     MMTVAPHDQELWIADPENCCSAVKVGQLDRA"
     assembly_gap    9485..9584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9755..10660
                     /locus_tag="PKY13_06570"
     CDS             9755..10660
                     /locus_tag="PKY13_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOT79633.1"
                     /translation="MDETTVASSTGTIDDASVPTRLLLLGLAHRNGDIVQHEVWPVTE
                     ACGLTADQVRSCLRRLVTEGLFVRSGEGRDAVYRATDEGSAVLSSMMARHVLAYAQDA
                     AGRGWDRTWRLVAFAIPESKRSARDVFRDRLLALGAAPVHNGLYVSPHRWDAEVDDEV
                     RRLGIEEHVSIATTDDLSVGGVSDPRALAAALWPLERIADRYREFIDTYRDVPDELDD
                     LRRRGERLSEADFLPGALHIAIRFNQCFDLDPMLPPELLPKPWPGREARDILARCRRL
                     GVLAREDKKGPALFRVFDDVIATLP"
     gene            10886..>11258
                     /locus_tag="PKY13_06575"
     CDS             10886..>11258
                     /locus_tag="PKY13_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79634.1"
                     /translation="MKFDVKQQFDADAAAVIGCYSSSEMYEQLPEFGKISRPDLLERT
                     ASGDTVKIRLRYKFTADLPTAALAIIDPDRLTWVEETTYDLAGLTSRLKLLPDHYASK
                     LEASASARFVDRGDGSIRDVAG"
BASE COUNT         1739 a         3932 c         3662 g         1625 t
ORIGIN      
        1 gcctcggacc acggaacgtg gagccgttgc ccggggaaga gactgtcgac cccgaagcgg
       61 tcgaggctga ccacccggag gcgcaaccac cgcccggtgt tcaccaactc ccatccgtgg
      121 tcgtcgaccg tgatcactcc gcagcagcgg tggagccgcg ggtcgtccgc cacgacgatg
      181 tcgacgtcgt cgccgccccg tccgaacgtc acccgttcac cgacggcgat ccgccgcccc
      241 catccgtctc cgaccagctc cagcactgtc gtcctccatc acccgatcac gtgtccgctc
      301 ggctgcgatt gccgtcgcga cgcccgccac ggtgaagcgc cgacgtcgaa aaggccagcc
      361 ggaatcggcc gtttggcatg ggggatttcc cccatcgcca ggtgccgacg cggcagtcag
      421 gatcgcccgc cgtatgcgtc tcggaagcga tgtggatcga ctcgacgggc tgtcactgct
      481 gctcgaggac ggggccgggt tgctcgtccg atcccggtcg gacctgttgt gggagaccgc
      541 cgcgaccgtg tggacgggcc cgagccgaca gcgcgtcgcg cacgacctcg aacgcacgat
      601 ggcgctcgcg gaccggatcg ccgagcgcct cgcaactctc ggccgggaag cccgtgccca
      661 gtccgaccga caacgacgag cgagcctcgc cggccgaccg acgatcgacg acgcacgggc
      721 gacgctgttg ttctccgacc tccacggcga cggacgcgcg gccatggcga tcggtgacgt
      781 ggccacggcg acgcacgtgg tcgtactggt gccggggatg ggcagcgacg ccagcaactt
      841 cgcggcgctg gtcgccgacg cccgacgcct ccagtaccac gccggcctcc ggctgggcgg
      901 cagcggctcg gtcgccgtcg tcgcgtggct cggctacgac gcaccggccg gactggacga
      961 ccccctcgcg ctgggccagg tgctcggccg agaccgggcg aacgccggag cgagcgcgtt
     1021 gcgaacgttc ctccgcgagc tcgatcgacg cgacgactcc tcggtcacgt tgatcggcca
     1081 ctcctacgga tcgctcgtcg tcgccggcgc agcccgggcg aacaccgagg tcgaccgggt
     1141 ggtggtgctc ggaagtccgg gcctcggggt cgccgacgtc ggcgggttgg gcctctcccc
     1201 cggcaccgcg ctctacgccg gcgccttcga caacgatccc gtcgcgcgga gcgagtggtt
     1261 cggtcgcgac ccgtcgtcgt tcgggttcgg ggcactgccg ctggcaacgg acccgtggcc
     1321 acaggacgat ccgccgagcg tgctgcacgc gcactccgcc tacttcgacg aggactccca
     1381 gtcgctcgac aacctcgcca ggctcgtcac cggcggggtg ccgcggggct cgatcgccgt
     1441 ggcgcgtcgc gtcaccggaa cgcgatgacg accgtcgccg caacggcgac gagcacggcg
     1501 acaaccacga ttgcgatcgt cgcagcgtcg gtgaaccccg cccaccggga gcccgatgtt
     1561 gccggctccg gcggcgtgga aggtgcggca acaggcgccg gcggctcggt cgggtcagcc
     1621 accgacgctt ccggccgggc cagcgaacgc gcacgcgtcg gtgccgaaga gacggccggc
     1681 tcgagttcca cgtgccggag gccgtcggcc atcgacgcga catcgggcca acgatccgcc
     1741 gggttcttcg ccatggcccg ttgcaggacg gcgaagagat ccgacggcac gtcggtgcgg
     1801 cgaagtggtg ggagcgggtc gtgcaggacc cgctggatca gaccggccat gccaccgggc
     1861 gagtccgccg taccgaacgg cggccggtcg tcgagcagcg tgtagagcgt cgacccgagc
     1921 gaccacacgt cgctcgacgg cccgctcggg ccgtcgccgt tcagccgctc gggcggggca
     1981 tggggcggcg acatcgagtc cttcgtgatc gtcgacgacg gcagggaacc tcccgctgcg
     2041 atatcgaagt cgccgagggc gacctcgccg gagtccgaca ccaggaggtt cgcgggcttc
     2101 acgtcacggt ggacgacacc acgggcgtga gcgtgaccga gcgcgacaca tgcggcgacg
     2161 ccgacgtcgc gaacccggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
     2281 gccgaagtcg acgagcaccc acttcttgtc gtcgagtccc tcgaccgaca tggggcggcg
     2341 gtcgaactcg acggccacgt ggtcctcgat ctcctgggcg accgccgcga cgaggcggga
     2401 gttccgggcc gagcagatca cgaagtagtc gcagatcccg atgatcgggc ccacgtcgag
     2461 gaccatcacc gaatccgcct gcttcgcgtc ggcggcgcga gccgcgacga ttgccacgtc
     2521 gtgggcgacg gtgttggtgg gcgtgcggtc actactcata ggggtgctcg gtgctgatcg
     2581 tgggtgatcg gtcgtcaggg ctgatggtcg gcgccgagca ccacatcgat gtcggctgcg
     2641 tcggagatct cgtcggtacg ctcggcgctg acgccgacgg cctcagcgat cgcatcggca
     2701 acgcctttga actcgtcgtt ggaatatacg acagtcgtgg acccgacatc gagcgacttg
     2761 gcgttgccga cgagcagcac ctcgccaccg gcccgggcga gatcgggggc aatccggctc
     2821 gccatcgtcg tgtcggtcgt gccgttgagg acccggacgc ggggccgctg gccggggaag
     2881 gaactcaccg ggaactgcac aacgccgttg agctgctcgg tgatcgcctc gggatcgggc
     2941 gagtagaggt acgagccctt gaacgactgc cgggtcgtcg gaaggacgat gaagtcgaac
     3001 gacgacgtgc ccatacgtgt gaccaacgtg cccaggtcgt tcgtctcgct gaccgacggg
     3061 gcggccgttg tgggcccccc ggtggtggtc tcgccggaac cgcccagctg ctcctgcagc
     3121 gccgtgagga gcgcctcggc acgcgtcgat cggttctcgg gccgctcgcc cgcaccgcgc
     3181 aacgtgaggt actcgccgag ctcgtcgggc gccagctcga tggtgccggc cttgaacttc
     3241 tcgaccttct tgcccttgac ctcgaccgag atcgggtcgg ggttcttcac cgacaccgtt
     3301 ccgagccccg ccgctgcgtc ggcaagttcg gtggggccga cgatcgtcgc gtcggtggac
     3361 ccgaaaccga gcaccttctc gaactcggcc cgggcggcgt cgagaccgcc ggacttgtac
     3421 acctcggcca gcgaggtcga cgcaccgtcg acctcgatca cgagctcgcc gaggctccag
     3481 acgatcgacc cactgccgcc cgccggatcg gggaccaaca cgaggaacgc gctgagcgcg
     3541 tcctggtcgt cggtgaccgc gacgaggtgg acgggcgacg cgtcgacagc ggcgacgaag
     3601 ccgggggcgg cggggtcggt gacgatcgtg gcggtcgccc cgcccttgat cttgcgggcc
     3661 gcgtcgtagc cctggacacc cagccacccg agcccgccga tcgcgagaac ggtcaggacc
     3721 gagaagatcc gcactgccag acgccggcgc ttccacgcgt gggcgcggtg caccacgggc
     3781 gcgtcgccgt cggcccgagg ggaggaggac gagccgctct cacctcgccc ggctccaccg
     3841 gggtgtcgag gctcggtcat cgtctcactc atcggcgcac accatagagg ccacgttgct
     3901 cgatcacgga gcggacggcg tcggggacga ggtagtcgat gggtgccccc gaagcgcacc
     3961 gggagcgaag atcggtgctc gagacctcga tcgcaggaac gtcgacgacg cgcagccgcc
     4021 atcgtggcga cgccgagggc gccggatggc cgggccgggc gaccgcgacg atcgacacca
     4081 tcgatgccag gtcctcggcg cggtgccacg aatcgaggcc cgcgacggcg tcggccccga
     4141 gcacgacggt gacgtcgacg tcgaccccag ccgtcagctg ctcgacggtg tcgaccgtgt
     4201 aggtgggtcc gccgcggccg atctccaggt tgctcgccac cagaccgtcg tgaccggcga
     4261 ccgccgccgc caccatctcg aaacgaaccg cagccggggt gatgtcgcgg gtcccgtgct
     4321 tctgccacgg gtcgttcgcg accatcagga gcacctcgtc gaacccgccg cgataccgaa
     4381 cctccgatgc cacgacgagg tgcccgacgt ggggcggatc gaacgtccca ccgaacaccg
     4441 ccaaccgtcg cgtcaccccc gaatcgtacg cgggcccggg ggcaccatcg gcatctgggc
     4501 cattcggccc aaaaatctca gggaaggcgc gaaaagtcgc tcaaccctgg ggaggacctg
     4561 ccgataggta cctcagagac gcacccagcg tgtcggtctc ggaatcaagc gtaggggcga
     4621 gtcgggtgaa ttccaccctc cccgcccgga aaattcgcct taccaggaag acagcgccac
     4681 gagcgcaggg tgatgacatg agcgattcag tcggtgtgta cctcaacgag atcggcaagg
     4741 tcccgctcct cacggcgatg gaggagatcg agctctccaa ggcgatcgag gccggcgtcg
     4801 aggcacgggc ccgcaaggag gccggcgagc gcggtgcgga catcaaccgc aagatcgccg
     4861 cggccaacgc tgcgaaggac cgcttcatcc gcgccaacct gcgcctcgtc gtgagcgtcg
     4921 cccgccgcta cccgctgccg tcgggcatgg agctgctgga cctcatccag gagggcaacc
     4981 tcggcctcga gcacgccgtc gacaagttcg actggcgcaa gggcttcaag ttctcgacct
     5041 acgccacctt ctggatccgc cagtcgatcg gccgggccct cgatcagaag gccagcctga
     5101 tccgccttcc cggtgaccgt tcggcctcgc tgcgcgccgc gctccgcgcc gtgtcgggcg
     5161 acggcgacga gctcgatacc gagaacgcgc tcctccaccg cctcgccacg cccacgtcgc
     5221 tcgaccgctc ggtcggccac gacgactcca acgaactggt cgacctgctc gccgacagcg
     5281 cccccgggcc cgaggcggag tacatggcgc aggtcgacga cgaactgctc gacaaggtgc
     5341 tgtcagtgct cgatccccgt gcccgcagcg ccgtggagca gcggttcggt ctcgccgacg
     5401 gctgccgtcg cagctaccgc gaggtcggcg aggagctggg tgtgaccgcc gaggccgccc
     5461 gacgcctcgt gaagcgggcc gtgaactcgg tgcgcgagac cgccctcgcc accatcgacg
     5521 ccgcgtagtc gccaattgat tgggcgggtc gcccgccagc cgcgacaatg gtcggatcat
     5581 gaccgctgag tggaatccat cgagctggcg cgagcgtccg gcccaacagc agcccgtgtg
     5641 gccggacgag tccgccaccg acgccgtgtt gaagacgctc aacggctatc cgccgctcgt
     5701 gttcgccggc gagagccgca acctcctctc cgaactcggc cgggtcgccc agggcgaagc
     5761 cttcctcttg caggccggcg actgtgccga gtcgttcgac gcgttcaacg ccacgtcgat
     5821 ccgcgagcgt ctcaagctca tcttgcagat ggcggtcgtc ctcacctact cggcaggggt
     5881 accggtcgtg aaggtgggcc gcatcgccgg tcagtacgcc aagccgaggt cgtcgaacac
     5941 cgaactcgtc gacggtgtgg aactgccgtc gttccgtggt cacatcgtca acgacccggc
     6001 gccgaccgcc tcggcccgga tccccgaccc gaaccgtctc atcgacgcgt accaccggtc
     6061 ggcctcgacg ctgaacctgc tgcgagcgtt caccaagggc gggttcgccg acatgaaccg
     6121 ggtgcacgcc tggaaccagg agttcgtcgc gtcgagcgcc gagggggccc ggtacgaggc
     6181 cctcgccgga cagatcgacc gggcgctccg cttcatggcg gcgtgcggca tcgacacgac
     6241 gagtgtgccg cgcctccacg aggtcgactt ctacaccagc cacgaggccc tcgtgctggg
     6301 ctacgaggag gccctcacgc ggcaggactc gttgaccggc gactggtacg actgctcggc
     6361 ccacatggtc tggatcggcg agcgcacccg ggggctcgac gacgcgcaca tcgagttctt
     6421 ccgcggcatc cacaacccga tcggctgcaa ggtcggcccg acggcgacgg tcgaggaggt
     6481 gctcgggctc tgtgaggcgc tcaaccccga gcgaattccc ggccggctca cgttgatcag
     6541 ccgcatgggc gccgacaagg tccgcgacca cctgccgccg atcctgagcg ccgttcgcga
     6601 cgccgggcac ccggtggtgt gggcgtgcga cccgatgcac ggcaacacct tcacctcgca
     6661 ggggggtcgc aagacccgcc acttcgacgc catcctcgac gagatccgcg ggttcttcga
     6721 ggcgcacgcc ggggtcggta cgtggcccgg cggtgtgcac gtcgagctga cgagcgacga
     6781 cgtgacggag tgcctgggtg gcgccgagcg actgggcgac tccgatctgg gcctgcgcta
     6841 cgagacgatc tgcgacccgc gcctcaacgg ccgtcagtcg ctcgacctcg cgttcgaggt
     6901 tgccgaactc cttcgtcgtt gaggcggctg acggcatttt tctccgcctt gcgctgttga
     6961 ggattctcga ccgggtcggt cgaggtaccg gagcgttagc agggagcgaa gcgaccgaac
     7021 ctagcgaagg tggccgagac cagtaaatgt tgaccctttt ggtcggaaat tgggatactg
     7081 gagatctccg ggggcgtaca gctcgcgggt gaggagtaca acctgtgacc accatcgaga
     7141 tcgatccgga tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaacctgtg
     7261 accaccatcg agatcgatcc ggatcttgcc aagcgaaccc agcgtgggga catcttcgag
     7321 gtccccgccg acatggccgc cgaaatcgaa gccgatatcg ccaagttcga gaagatgctc
     7381 gccctgcacc tcgccggcga gatcgaggac gacaccttcc gggtcttccg cctcaacaac
     7441 ggcatctacg gccagcgcca gggcggcacc aaccagatgg tccgggtcaa ggcaccgtac
     7501 ggcaagctca acgcgcagca gctcgacctg ttcgcccgcg tcgccgacga gtactcccgc
     7561 gggtggggcc acatcacgac ccgccagaac ttccagttcc actacgtcga gctcacccgg
     7621 gtgcccgacc tcctcaagga gttcgcggcc ctcggcatga cgacccgtga ggcgtgcggc
     7681 gacaccgtcc gcaacgtgca gggctgccac ctcgccggcg cctgccccca cgagaagctc
     7741 gacatcaccg cgtgggccga ggcggcgcac cgccacttcc tccaccaccc cttcgcccag
     7801 cgcctgcccc gcaagttcaa gatcaacttc agtgggtgtg agaccgactg cggccaggcg
     7861 atgttcaacg acgccggcgt gatcgccgtg acgcgcacac tcgaggacgg cacggtcgag
     7921 gccgggttcc gggtgttcgt cggcggcggc ctcggggcca acccccacgc cgccctggca
     7981 ctcgaggagt tcacccctcg cgagaacctg ctcccgacca tcgaagcgat cctccgcatc
     8041 ttcgagcagc acggcaaccg caagaacaag ctgcgtgccc gcctcaagtg ggttatccag
     8101 gacctcgggt gggacgaggt gcaggctcgc atcctcaact cccgccgcct cctcccggcg
     8161 tcgtccacct ggcccggcgg catccccgag gtcgtgcagc agtggggcga cacccccgct
     8221 ggtgtggccg ccggcgtgac cccgaccgtc atcggccaag gcacgcccgt cgcactgcgt
     8281 tccaccaagc cgtacgagcg gtgggagcag gcgaacgtga tccgcggttc ggccaacggc
     8341 accgtgtcgg cgatcgcctg gtcgcgcctc ggcgacgtca cgtcggacca gttccgcggc
     8401 ctcgcgtcga tcacccgtga gttcaacgcc gaggtgcgca tcaccaaccg ccagaacctc
     8461 gtgttccgcg acctcaccga ggagcagctg ccgctcctgt tcgagcgact cacggccatc
     8521 ggcatggccg agccgggcgc cgagctgagc cgcgacgtcg tcgcctgccc cggcgccgac
     8581 acctgcaaca tcgcggtcac ccagtcccgc ggactcgccg acgccatcgg tgtggcgctc
     8641 gaagaggccg gcctcggcga ggtcggcggc atccgcacca acatcagcgg ttgcaccaac
     8701 tcgtgcggcc agcaccacac gtccgacatc gggttcttcg gtgccgaacg tcgcgcccac
     8761 ggcaaggcgg cccccggcta ccagatgctc ctcggcggat acgtcggcca ggagcaggtg
     8821 cacttcggcg agaaggccct gcgcctcccg gccaaggcgg caccacaggc cgtggtccgc
     8881 gtcgtcgggc gcttcaacga cgagcgccag gccggcgaga cgttccggtc gtggatcgag
     8941 cgcgtcggtg gggtgaagga gatcgcggcg acgctcaagg atctcgacat cttccccacc
     9001 cccgaggacg gccccgattt ctacgtcgac tacgacgaga ccggtccgtt cgaggtcgag
     9061 atcggcgact cggagtgcgc cgtctgatga gcgagacgtc gctgtcggtg ccctcggcgc
     9121 gctttgctcc cgccgagttc tccgacgagg agctcgccga cctcaactcc gacttcgaac
     9181 gccttccggc gtcgaagatc atccagtggg cggtcgactc gttcgggccc tacctgtcgt
     9241 tggcggcgtc gatggccgac gcactcgtca tcgatatcgc caccagggtc gacccggcga
     9301 tcgaggtcgt cttcatcgac accggctacc acttccccga gacgctcgag accgtcgaga
     9361 cgatccgtcg ccactacggg ctcaacctcc gcatgatgac ggtggcaccc cacgaccagg
     9421 agctgtggat cgccgacccg gagaactgct gctcagcggt caaggtcggt cagctcgacc
     9481 gggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctccg cttcgctccg
     9601 gaaccgctcg gcctgccagc ctcgtcgctc gtcctcaggc tcgctccctc gtccctcggg
     9661 agcttcttac ggacggctcc ttcggcggcc cgccacgcgt gcggcgcgac gaccgcagct
     9721 tgccctcccg cagtcgccat gacattcttc ggtcgtggat gaaacgaccg ttgcaagttc
     9781 gaccggcacg atcgacgacg cgtcggtgcc cacccggttg ctcctgctgg gcctggctca
     9841 tcgcaacggc gacatcgtcc agcacgaggt gtggcccgtc accgaggcgt gcggcctgac
     9901 cgccgatcag gtgcggtcgt gtctccgtcg cctggtgacc gagggcctgt tcgtccgcag
     9961 tggcgaaggt cgcgacgcgg tctaccgcgc gaccgacgaa ggcagcgcgg tgctgtcgtc
    10021 gatgatggcc cgccacgtgc tcgcgtacgc gcaggacgct gccggccggg gttgggaccg
    10081 cacgtggcgc ctggtcgcgt tcgcgatccc cgagtcgaag cgctccgctc gcgacgtgtt
    10141 ccgcgatcgc ctcctggcgc tcggcgctgc cccggtgcac aacggcctgt acgtgtcacc
    10201 ccaccgctgg gatgccgagg tcgacgacga ggtccgccgg ttgggcatcg aggagcacgt
    10261 gtcgatcgcg accaccgacg acctgagcgt cggtggggtc agtgatcctc gagccctcgc
    10321 agccgcgctc tggccgctcg agcgcatcgc cgaccgctat cgcgagttca tcgacacgta
    10381 tcgcgacgtg cccgacgaac tcgacgacct ccggcgccgg ggcgagcgcc tctcggaggc
    10441 ggacttcctg cccggcgccc tgcacatcgc gatccgcttc aatcagtgct tcgacctcga
    10501 cccgatgctg ccgccggagc tcctgccgaa gccatggccc ggccgcgaag ctcgcgacat
    10561 cctcgcccgt tgtcggcgcc tgggcgtcct cgcccgcgaa gacaagaagg ggcccgcgct
    10621 cttccgggtg ttcgacgacg tcatcgcgac cctgccgtag ccggtcgggg gcgcccgaag
    10681 tggtgcgctg gcgcgacgtt tccatcgcgc tccggcacca cttcactcgg ccacggtcca
    10741 gcgataccca cgatcaggtc aagactcggc ccagctctgc gcctccaggc tggacgcaac
    10801 acgcgcccga tgtgacgcgt cgcgtcggat cgggcgcgga ttacccggcg caggtcgcac
    10861 tcgacgaccg tagagtgacc agcgcatgaa gttcgacgtg aagcagcagt tcgatgccga
    10921 cgccgcggcc gtcatcgggt gctactcgtc ctccgagatg tacgagcagc tgcccgagtt
    10981 cggaaagatc agccggcccg acctgctcga gcgcaccgca agcggcgaca cggtgaagat
    11041 ccgcctgcgg tacaagttca ccgccgacct gccgaccgcg gccctggcaa tcatcgatcc
    11101 cgaccgtctc acctgggtcg aggagacgac gtacgacctc gccgggctca cgtcgcgcct
    11161 gaagctcctc cccgatcact acgcgtcgaa gctcgaggcc tccgcgtcgg cccgcttcgt
    11221 cgatcgtggc gacggctcga ttcgcgacgt cgccggcg
//
LOCUS       DAOSOA010000073        11247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5759_length_11247_cov_6.380540, whole genome shotgun sequence.
ACCESSION   DAOSOA010000073 DAOSOA010000000
VERSION     DAOSOA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5759_length_11247_cov_6.380540"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..263)
                     /locus_tag="PKY13_06580"
     CDS             complement(69..263)
                     /locus_tag="PKY13_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79635.1"
                     /translation="MTAPTKLIVFGIALVALFLASFTIAGAVVPDDVVTRWTERGATH
                     DTGVTGTGTTHTGHTDGHGS"
     gene            complement(260..472)
                     /locus_tag="PKY13_06585"
     CDS             complement(260..472)
                     /locus_tag="PKY13_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOT79636.1"
                     /translation="MQNHTYRVTGMTCGHCEAAVRSEVEGIDGVESVDVQLDTGELVV
                     RGSAEVTDEAVLAAVEEAGYDAVRVG"
     gene            661..1290
                     /locus_tag="PKY13_06590"
     CDS             661..1290
                     /locus_tag="PKY13_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOT79637.1"
                     /translation="MRLRQRLPLIPLVLAIALAAGACTVPSPAPSEKTIAQQLLSDTG
                     DNAAGFDDDWYDFDIATQAILLYPDLVEAASDPSASLTVFLPNDRAFQVLVYSLTGNV
                     VLDEQGVFDAVASLGLPTVKTVLTYHIVGSKISATDALAANGAELTTLQGGKLKVDVV
                     NPALSLILLRDLDPNADDAGVVYSKFNYGGSLANGYIHGLSLVLRPADL"
     gene            1466..2023
                     /locus_tag="PKY13_06595"
     CDS             1466..2023
                     /locus_tag="PKY13_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT79638.1"
                     /translation="MDSNHDADAVLAERFRSGDERALEEVYREHSRLVYSFCRRALDD
                     HRAADATQDVFLAAWRSRERYRPDLGSLAGWLMGIARFKVVDIHRTEQRNPLAPVTAQ
                     DAERGVVAEDLDLMAQRMLIADALMDLPERSREMVRLAYFDDQTHTQIAERSGVPLGT
                     VKSDIRRGLQRLRRHLEAFDDAPRS"
     gene            2004..2660
                     /locus_tag="PKY13_06600"
     CDS             2004..2660
                     /locus_tag="PKY13_06600"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOT79639.1"
                     /translation="MTHHDPEHPSPESDRRLSELLGGLTDADFERVDPPADLLPRITA
                     HAAAERDAASGADAAASGSSAPVVISMDERRRPRPFLFAAAAAVLLVVAAVGAILATR
                     APARTERQLARAELEQLEPLGSTRATARLVEENGTTHLRIDASDMAPAPAGSKYELWL
                     IDRGVTDPRSLGTITGTEDVVVPSSIDPDEYAIVDISLEPDDGDHAHSGHSLMRGTLA
                     "
     gene            complement(2747..3289)
                     /locus_tag="PKY13_06605"
     CDS             complement(2747..3289)
                     /locus_tag="PKY13_06605"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOT79640.1"
                     /translation="MKRSLVASVLVLSSLATACAPMLPTRTSTVPSVDVSRYLGTWYE
                     VASVKQFFSIGLVGSTAEYSLNPDGSIRVFNSGNYFTFDGPPSSIVGSAVPVDSSNAR
                     LNVSFTGFNSAEGAGNYWIVDLDPDYQWAIVSDPTGVSCFLLSRTRSISPELRADLLA
                     RAAAKGVDISNVTETLQPPA"
     gene            3447..4400
                     /locus_tag="PKY13_06610"
     CDS             3447..4400
                     /locus_tag="PKY13_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOT79641.1"
                     /translation="MKRSWIVRMAGAGLALTLLSPMASAGAAPVTAAAAPTGDIVATA
                     QAAGQFSVLLEAAQKAGLVDALKGPGPYTVFAPTDAAFSAALTKLGISKDQLLNSGPI
                     LAAILKYHVLSGAVPSSAITGPLGPTTLEGRQVLVVPKDGKVTLNLSATVITADVEAT
                     NGVIHVIDQVLLPPAHEPDIVDTAANAGSFSTLIAAAKAAGLVDALKAPGPYTVFAPT
                     DAAFRTLLRRLRTTPEKLLADPAKLASILKYHVVSGEITSSQLKVGRNVVTTLNGNKL
                     VVRKYKNGRVTVNGTALVTTADVDASNGVIHVINRVLVPRS"
     gene            4487..5161
                     /locus_tag="PKY13_06615"
     CDS             4487..5161
                     /locus_tag="PKY13_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOT79642.1"
                     /translation="MTDDDAPRPEDCLSPTTYLDADHPDIVDFVERVCAEAGATTERE
                     RAVALFLAVRDGIRYDPYAVSGDRDWYKASSVLHHPAAYCIPKAILLSAACRAAGIPA
                     RLGFADVRNHLQSEKLRESMGTDVFAYHGFSELWLGGRWLKVSSAFNRELCDRFGTQV
                     LEFDGEHDALMHPYDRQGNRHMEYVRSRGSFADLPFDEIMAGFAEVYGQMDDYGGDRA
                     DEAFQA"
     gene            5201..>6367
                     /locus_tag="PKY13_06620"
     CDS             5201..>6367
                     /locus_tag="PKY13_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79643.1"
                     /translation="MSDPPPDPTVAAPLLRTKLHAPSRRRGAVDRPRLAVRAPTGERR
                     RLTLVSAPAGFGKTTLLSEWYAGGAGSGTAWLSLDGRDNDPGTFWTYVIAALRTVAPE
                     VGAAALAILETSTGPTDAAVESLVGDLDALDADVVLVLDDYHVIDEAEIHDAVGFLID
                     HLPPSVGLVLATRVDPPLPLARLRASGDLVERRAADLRFTPDETARYFNTTMGLALSV
                     DEVGALEARTEGWIAALQLAALSLQGRDDVAGFIESFTGDDRFVVDYLIEEVLDRQPP
                     DVRQFLLRTAVLNRLSGSLCDAVTGGTSGRATLERLDRANLFLVPLDDRRHWYRYHHL
                     FADVLRARLLDEQPALVPELHRQAARWWADEGDLVEAVGHAMAGADVELAAELIE"
     assembly_gap    6368..6467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6468..7523
                     /locus_tag="PKY13_06625"
     CDS             <6468..7523
                     /locus_tag="PKY13_06625"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT79644.1"
                     /translation="ADALGCSLGLADMCLAQGHLHGAERACNAGLDLVGAHPGLRGAA
                     DMHIGLCEVCIERNDLGRAREHLAAGVALGERAGLPQYPYRWRVAAARLRRAEGDLGG
                     ALALLDEAKDRYDTDYSPSVRPVPALEARVRLARGELDAALRWAEQQHLSVGDELAYV
                     REFEHLTLARVLVAVEARDRSDLGAVGLVERLMSAAEASGRTGVVIECLVLLSLARSA
                     TGDRSGALTAVEQALVLAEPEGFVAVFLEAGEPLRTLLRTASFDGVAASQCRVVLAAW
                     SGAEPVRFASGPVDELSSRELDVLRLLRSELGGPEIARELMVSLNTMRTHTKNIYAKL
                     GVTSRREAVRRADQLGL"
     gene            7601..7861
                     /locus_tag="PKY13_06630"
     CDS             7601..7861
                     /locus_tag="PKY13_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79645.1"
                     /translation="MTAPQPPIYEIRVAGRLSSNWDAWFDGLSVTSDPDGTSVIRGSV
                     VDQAALHGLLQKLRDVGLVLESLTQVGIADPTDHHHQPGATS"
     gene            7858..8754
                     /locus_tag="PKY13_06635"
     CDS             7858..8754
                     /locus_tag="PKY13_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT79646.1"
                     /translation="MINVSGLTKRYGDHTAVDALSFDAAAGRVTGFVGPNGAGKSTTM
                     RMMVGLTRPDAGEVRYGGTRYTDLVHPARAVGAVLDPRCMHPNRTARNHLRAMAAASK
                     IPRDRVEQVLVEVGLESVMDQKAGQFSLGMRQRLALAGAMLGDPPVLLLDEPSNGLDP
                     DGIRWLRNHLTAYAERGGTVFVSSHLISELSMFADDLVVVGAGRLLAAETVEAITSRS
                     ATSVVVETPQQEELLQLLDARGLAVDTTDDRLTVHGTTKAEISQLAFEHGIRLLELTE
                     ITQSLEDSLLAMTGASAEFTAV"
     gene            9186..9644
                     /locus_tag="PKY13_06640"
     CDS             9186..9644
                     /locus_tag="PKY13_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79647.1"
                     /translation="MAKTVMAVAVGATYGAAAMAAGFAGALAGGAELGDGSAIPARAA
                     WALLYIALAAVIGLGVGMIVRHSAGAISGLLMWSFVIETLFAPALPDGVLHFLPFSAG
                     YRLLDAGADFKAPVVIADELSRPMYAVIFGGYALVTLAVGTLLLARRDTN"
     gene            9713..10087
                     /locus_tag="PKY13_06645"
     CDS             9713..10087
                     /locus_tag="PKY13_06645"
                     /inference="COORDINATES: protein motif:HMM:NF023331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79648.1"
                     /translation="MAKSTSVPSRITADVAAAAAAIAPGEHRTITEQINYWARIGMQV
                     ERSGSLASRRVLAVAAGAEQFSGLATEERLAAHALIDAGIAERAASERFGPAARAAGQ
                     TTVSIEDGHLVEISPGGARRRL"
     gene            10084..10653
                     /locus_tag="PKY13_06650"
     CDS             10084..10653
                     /locus_tag="PKY13_06650"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOT79649.1"
                     /translation="MSRLDLIVGPNGAGQTTLYERVIAPGRPGLPFVNADRIAADRFG
                     DEAEARSYEAAEIAEAARDALIAARLDFCSETVFSHSSKVDLVAGAVEAGYEIVLHVV
                     MIPLELSGPRVAVRVAAGGHSVPQGKLAARYRRLWPNVAAAVPLCRRAVFWDNSSRDG
                     PVEVAAFRLGVADFPPRWPDWTPEELRLL"
     gene            10721..>11247
                     /locus_tag="PKY13_06655"
     CDS             10721..>11247
                     /locus_tag="PKY13_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79650.1"
                     /translation="MTNTSSYRSQLAGQLRDAAVPLRTLLSDLSPADVPLPYAVDILD
                     AFIELERLAASGKVLIAARAAESEAWRRAGSPSAAAWLALRQGTDTGRARADLETSQQ
                     LGGLTGTADAVRNGELSPQQAGAVAAGASVNPAAEDDLLETAKSDSLRHTKEEAARRR
                     AEVEGEESKVERERKV"
BASE COUNT         1700 a         4034 c         3713 g         1700 t
ORIGIN      
        1 gcgcatggcg cacgaggcgc aggtcatgcc gccgatctcg agctcgatgt cggccgtgct
       61 gggcgtggtc acgatccgtg tccgtccgtg tgcccggtgt gggtggtgcc ggtaccggtg
      121 acacccgtgt cgtgggtggc gccgcgctcg gtccagcggg tcaccacgtc gtcggggacc
      181 accgcgccgg cgatcgtgaa cgatgcgagg aacagcgcga cgagcgcgat gccgaagacg
      241 atcagcttcg tcggagcggt cacccgaccc tcacggcgtc gtaccccgcc tcctcgacgg
      301 cggcgaggac ggcctcgtcg gtgacctcgg cgctgcctcg gacgacgagc tctccggtgt
      361 cgagctgcac gtcgaccgac tcgacgccgt cgatcccttc gacctcggac cgcacggcgg
      421 cctcgcagtg gccgcaggtc atcccggtca cccggtacgt gtggttctgc atggtgtcct
      481 cctcggtcga tggatacccc tgtggggtat cgctacggcc ccgacgatat acctgcaggg
      541 ggtatgggtc aatcgccccg gcggccgggg gtgcccgggc ggtcaccaca acgttgcatc
      601 cgcaagggcc ccgtcgaccg aaccacctct tgaccccgac cacgaccgaa aggccgctcc
      661 atgcgacttc gtcaacgact cccactcatc cccctcgtcc tcgccatcgc gctcgccgcc
      721 ggtgcctgca cggtgccgtc cccggcgccg tcggagaaga cgatcgccca gcagctgctg
      781 tccgacaccg gcgacaacgc cgccggcttc gacgacgact ggtacgactt cgacatcgcc
      841 acccaggcga tcctcctcta ccccgacctc gtcgaggctg cgtccgaccc gtcggcgtcc
      901 ctgacggtgt tcctgcccaa cgaccgggcg ttccaggtgc tggtgtacag cctcaccggc
      961 aacgtcgtcc tcgacgagca gggtgtgttc gacgccgtcg cctcgctcgg cctgcccacc
     1021 gtcaagaccg tgctcacgta ccacatcgtc ggttcgaaga tctccgctac cgacgccctc
     1081 gcggccaacg gtgccgagct caccaccctg cagggcggga agctgaaggt cgacgtggtg
     1141 aaccccgcgc tcagcctgat cctgttgcgc gacctcgacc cgaacgccga tgacgccggt
     1201 gtcgtgtaca gcaagttcaa ctacggcggc tccctcgcca acggctacat ccacggcctc
     1261 tccctggtgc tgcgccccgc ggatctctga tccgaccgtc ctcccaccgt gatgcagggg
     1321 ccgcagctgt gagaagctgc ggccctatgc atccggatag ggcggtgact ccgaacgact
     1381 caatgtggct gtcgacacct acgaacgaag cgctgtggcc gtcgatgtgc tcgaacgcgt
     1441 ccggatgagc gatcggactg ttcgaatgga ttcgaaccac gacgccgatg ccgtgctcgc
     1501 cgagcgattc cgttccggtg acgagcgggc cctcgaggag gtctaccggg agcacagtcg
     1561 cctggtgtac tcgttctgcc ggcgggcgct cgacgaccac cgtgccgccg acgccaccca
     1621 ggacgtcttc ctcgctgcct ggcggtcgag ggagcggtac cgccccgacc tcggttccct
     1681 ggccggttgg ctcatgggca tcgcccgctt caaggtggtc gacatccacc ggaccgagca
     1741 gcgcaacccg ttggcaccgg tcaccgcaca ggacgccgag cgtggcgtcg tcgcagagga
     1801 tctcgacctg atggcccaac gcatgctgat cgccgacgca ctcatggacc tcccggagcg
     1861 atcacgggaa atggtccgcc tcgcctactt cgacgaccag acccacaccc agatcgccga
     1921 gcggagcggt gttccgctcg gcacggtcaa gagcgatatc cgacgagggc tccagcggct
     1981 ccgtcgacac ctggaggcgt tcgatgacgc accacgatcc tgagcacccg tcgcccgaga
     2041 gcgaccgccg cctgtccgag ctgctcggcg gcctgaccga cgccgacttc gagcgggtcg
     2101 atccgccggc cgacctcctc ccccgcatca ccgcccacgc cgctgccgag cgcgacgccg
     2161 cgtccggcgc cgacgctgcg gcgtcgggtt cgtcggcacc cgtcgtgatc tcgatggacg
     2221 agcgccgtcg tccccgcccg ttcctgttcg ccgctgccgc cgccgtgctg ctcgtggtcg
     2281 cggccgtcgg agcgatcctc gcgacccgcg ccccggcccg aaccgaacgt caactcgccc
     2341 gcgccgagtt ggagcagctg gagccgctcg gttccacccg cgccacggca cgcctcgtcg
     2401 aggagaacgg gaccacgcac ctgcgcatcg acgcgagcga catggcgccg gcgcccgccg
     2461 gttcgaagta cgagctctgg ctgatcgatc gtggcgtgac cgatccccgg tcgctcggca
     2521 cgatcacggg caccgaggac gtcgtggtgc cgtcgtcgat cgaccccgac gagtacgcca
     2581 tcgtcgacat cagcctcgag cccgacgacg gcgaccacgc gcactcgggc cacagcctga
     2641 tgcgcggcac cctcgcctag cgcgttgttc ccacaacgcg aacggtgcgc cggcacgacg
     2701 tgatcgtcgc accgacgcac cggtgatcag gaacgcaatc gggtgatcag gcggggggct
     2761 gcagggtctc ggtcacgttg ctgatgtcga cgccctttgc cgcggcacgg gcgaggaggt
     2821 cggcgcgcag ctccggcgag atcgagcggg tgcggctcag gaggaagcac gagacaccgg
     2881 tcgggtcgct gacgattgcc cactggtaat cggggtcgag atcgacgatc cagtagttgc
     2941 cggcgccctc ggccgagttg aagccggtga acgacacgtt gagtcgggcg ttcgagctgt
     3001 cgaccggcac ggccgaaccg acgatgctcg acgggggacc gtcgaaggtg aagtagttgc
     3061 cggagttgaa cacccggatc gatccatcgg ggttgagcga gtactcggcg gtgctgccga
     3121 ccagtccgat cgagaagaac tgcttcacgc tcgcgacctc gtaccacgtg ccgaggtagc
     3181 ggctgacgtc gaccgacggg acggtcgacg tccgggtcgg caacatcggc gcgcacgccg
     3241 tggcgagcga gctgaggacg agtacggacg cgacaagaga acgcttcatg agagtcatac
     3301 cggtgcttcg gtcgatccgc ccccaacgga tgcacccgcc ggacctgagc ggcggggtga
     3361 ttctgccgtg gtgcatccga acgtgttgcg ccgcccgaac gaccggtgaa ccaccgaccg
     3421 gtggtgtgac tgaacggagg aatcgaatga agagatcctg gatcgttcga atggccggcg
     3481 ccgggctggc gctgacgctg ttgagcccga tggcctcggc cggggcggca ccggtgacgg
     3541 cggcggcagc gccgaccggc gacatcgttg ccaccgcgca ggctgccggg cagttctcgg
     3601 tgctgctcga ggcggcgcag aaggcgggcc tcgtcgacgc gctgaagggc ccgggccctt
     3661 acaccgtctt cgctcccacc gacgcggcgt tctccgcggc gctcaccaag ctcggcatca
     3721 gcaaggacca gttgctcaac agcgggccca tcctggcggc gatcctgaag taccacgtcc
     3781 tgagcggcgc cgtgccgtcc tcggcgatca ccggcccgct cggccccacc acgctcgagg
     3841 gtcgccaggt cctcgtcgtg cccaaggacg ggaaggtgac cctgaacctg agcgcgaccg
     3901 tgatcacggc cgacgtcgag gccaccaacg gggtcatcca cgtcatcgac caggtgctcc
     3961 ttccgccggc ccacgagccc gacatcgtcg atacggctgc gaacgccggg tcgttctcga
     4021 cgctgatcgc cgccgcgaag gcggccggcc tggtcgacgc cctcaaggcc cccggcccct
     4081 acacggtgtt cgcgccgacc gacgctgcgt tccgtacgct cctgcgtcgc ctccgcacga
     4141 cgccggagaa gctgctggcc gatccggcca agttggcctc gatcctcaag taccacgtgg
     4201 tgtccggtga gatcacctcg tcgcagctga aggtggggcg caacgtcgtc accacgctca
     4261 acggcaacaa gctggtcgtc cggaagtaca agaacgggcg ggtgacggtc aacggcacgg
     4321 ccctcgtcac caccgccgac gtcgacgcct ccaacggcgt catccacgtc atcaaccggg
     4381 tcctcgtccc ccggtcctag cggcgtcgtt cgccggtccg gcatcggggt cagccccgtt
     4441 gccggaccgg agcgcccgac ctgcgcatcg tggcaggctg ggagacatga ccgacgacga
     4501 tgctccccgg cccgaggatt gcctgtcccc cacgacctac ctggacgccg atcatcccga
     4561 catcgtcgac ttcgtcgagc gggtgtgcgc cgaggccggg gcgacgaccg aacgggagcg
     4621 ggcggtggcg ctcttcctgg ccgtgcgcga cggcatccgc tacgacccct acgcggtgag
     4681 cggtgatcgc gactggtaca aggcgagcag cgtcctgcat cacccggcgg cgtactgcat
     4741 ccccaaggcc atcctgctct cggcggcctg ccgtgccgcc ggcatcccgg cccgtctcgg
     4801 gttcgcggac gtccgcaacc acctgcagag cgagaagctg cgcgagagca tgggcaccga
     4861 cgtgttcgcg taccacggct tcagcgagct gtggctgggc ggacggtggt tgaaggtcag
     4921 ctcggcgttc aaccgtgagt tgtgcgaccg gttcggcacc caggtgctgg agttcgacgg
     4981 ggagcacgac gcgttgatgc acccctacga ccggcagggc aaccgccaca tggagtacgt
     5041 gcgatcccgg ggcagtttcg ccgacctgcc gttcgacgag atcatggcgg ggttcgccga
     5101 ggtgtacggc cagatggacg actacggcgg cgaccgggcc gacgaggcct ttcaggcctg
     5161 aaccgccgcc gttccccgcc gtccctggaa gactgcgggg atgtccgacc ctccgcccga
     5221 cccgacggtc gccgcgccgc tcctgcgcac caagctgcac gcgccgagcc gtcgccgtgg
     5281 cgccgtcgac cggccgcggc tcgccgtgcg ggcgcccacc ggcgaacgcc ggcgcctcac
     5341 gctcgtgtcc gcgccggccg ggttcgggaa gacgacgttg ttgtcggagt ggtacgccgg
     5401 tggggccgga tctggcacgg catggctgtc gctggacggg cgcgacaacg atccgggcac
     5461 gttctggacc tacgtgatcg cggcattgcg caccgtcgca cccgaggttg gggccgccgc
     5521 gctcgccatc ctcgagacct cgaccgggcc gacggacgct gcggtggaat cgctcgtcgg
     5581 cgatctggac gccctcgacg ccgacgtggt cctcgtgctc gacgactacc acgtgatcga
     5641 cgaagcggag atccacgacg ccgtcgggtt cctgatcgac catctgccgc cgagcgtcgg
     5701 cctggtgctg gcgacccggg tcgatccacc gctcccgctg gcgcgactcc gggcgagcgg
     5761 ggatctcgtc gaacgtcggg ccgcggacct gcgcttcacg cccgacgaga ccgcccgcta
     5821 cttcaacacc acgatgggcc tcgcgctcag cgtcgacgag gtcggcgcac tcgaggcccg
     5881 caccgagggc tggatcgcgg cgctccagct cgcagcgctc tcgttgcagg gccgcgacga
     5941 cgtcgcggga ttcatcgaga gcttcaccgg tgacgatcgg ttcgtcgtcg actacctgat
     6001 cgaggaggtg ctcgaccgcc agccgcccga tgtccgccag ttcctcctcc gtacagcggt
     6061 cctgaatcgg ctcagcgggt cgctctgcga tgccgtcacc ggagggacct ccggtcgggc
     6121 gacgctggaa cgactcgacc gggcgaacct gttcctcgtc cccctcgacg atcgccgcca
     6181 ctggtaccgc tatcaccatc tcttcgccga cgtcctccgt gcccgcctcc tcgacgagca
     6241 accggccctc gtgcccgagc tgcaccggca ggcggcgcga tggtgggcgg acgaaggtga
     6301 cctcgtcgag gcagtcggtc acgccatggc gggagccgac gtcgagttgg cggcagagct
     6361 catcgagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gacgcgctcg
     6481 gctgctcgct cggcctcgcc gacatgtgcc tcgcccaagg gcatctgcac ggtgcggagc
     6541 gggcgtgcaa cgccggcctc gatctcgtcg gcgctcatcc cggcctccgt ggtgccgccg
     6601 acatgcacat cgggctctgc gaggtgtgca tcgaacggaa cgacttggga cgggcgcgtg
     6661 agcacctcgc cgcgggcgtc gcgctcggcg agcgggccgg actcccgcag tatccctatc
     6721 ggtggcgggt cgcggcggca cgcctgcgtc gcgccgaggg cgacctcggc ggtgcattgg
     6781 cgttgctcga cgaggcaaag gatcgctacg acaccgacta ctcgccgtcg gtccggcccg
     6841 tgcccgcact cgaggcccga gtgcggttgg cccgcggcga gctcgatgca gcgctgcgat
     6901 gggccgaaca gcagcacctg tccgtcggcg acgagctcgc ctatgtccgc gagttcgaac
     6961 acctcaccct ggctcgggtg ctggtggccg tcgaggcacg cgaccggtcg gacctcggcg
     7021 cagtcggtct ggtggagcga ttgatgtcgg cggccgaggc cagcggccgc accggcgtgg
     7081 tcatcgagtg cctggtgctc ctgtcgctgg cccgctccgc caccggcgac cggtcaggtg
     7141 cactgaccgc ggtcgagcag gcgttggtgc tcgccgaacc cgaggggttc gttgcggtgt
     7201 tcctcgaggc cggcgaaccg ttgcggacgc tgctgcgcac ggcgtcgttc gacggggtcg
     7261 ccgcatcaca gtgccgggtg gtgcttgccg cctggtcggg ggcggaaccg gtgcggttcg
     7321 cgtcgggtcc cgtcgacgaa ctcagcagtc gtgagctcga cgtgctgcgc ctgctccgca
     7381 gcgagctcgg cggccccgag atcgcccggg agctcatggt gtcgttgaac acgatgcgca
     7441 cccatacgaa gaacatctac gccaagctcg gcgtcaccag ccggcgtgaa gccgtgcgcc
     7501 gcgccgacca gctcggtctg taggcgcccg cggcaatcac cacccacgat caccacacct
     7561 ggtgatcaca gctcaccagg tcgcttcgta cggtcgaccc atgaccgcac cgcaacctcc
     7621 gatctacgag atccgagtcg ccggccgcct gagctcgaac tgggacgcct ggttcgacgg
     7681 actgagcgtc acgagcgacc ccgacggcac ctccgtcatc cgggggtcgg tcgtcgacca
     7741 ggccgcgctg cacggcctcc tccagaagct gcgcgacgtc ggcctcgtgc tcgagtcgct
     7801 cacccaggtc ggcatcgccg accccaccga tcaccaccac caaccaggag ccacatcatg
     7861 atcaacgtct caggactgac caagcgctac ggcgaccaca ccgcggtcga cgccctctcg
     7921 ttcgacgccg ccgccggacg cgtcacgggg ttcgtcggcc ccaacggagc cggaaagtcg
     7981 accacgatgc gcatgatggt cgggctgacc cggcccgatg ccggcgaggt gcgctacggc
     8041 gggacccgct acaccgacct cgtgcatcct gcccgtgcgg tcggcgcagt gctcgacccc
     8101 cggtgcatgc acccgaaccg cacggcccgg aaccatctgc gagcgatggc tgcggcgagc
     8161 aagatcccga gggatcgggt cgaacaggtg ctcgtcgagg tcggcctcga gtcggtgatg
     8221 gaccagaagg ccggacagtt ctcgctcggg atgcgtcagc gtctcgctct cgccggcgcc
     8281 atgctcggcg atccgccggt cctgctgctc gacgagccgt ccaacgggct cgatcccgac
     8341 ggcatccgct ggctgcgcaa ccacctcacc gcctacgccg agcgcggcgg cacggtgttc
     8401 gtgtcgagcc acctcatcag cgagctcagc atgttcgccg acgacctcgt cgtcgtcggt
     8461 gccggccggc tgctcgccgc cgagaccgtc gaggccatca cctcgcgctc ggcgaccagc
     8521 gttgtcgtcg agacgcccca acaggaggaa ctcctgcagc tcctcgacgc acgcggcctc
     8581 gccgtcgaca cgaccgacga ccggctcacc gttcacggca cgaccaaagc ggagatctcc
     8641 cagctcgcgt tcgagcacgg catccgcctc ctcgagctca ccgagatcac gcagtcgctc
     8701 gaggacagcc tcctcgcgat gaccggcgcg tccgccgagt tcaccgccgt ctgaccaccc
     8761 gaccgaccgt cgttcccgac cgaacccatt ccgaccagaa actcaccaag gaccacgaac
     8821 catgaccacc accatcatca ccccacgacc cgagaccagg accgacacgg tcctccgaag
     8881 ccggaccgac gacgtccacg ccgtccccgc tgccctgcga agcgagtgga tcaaggtgtc
     8941 cacgctgcgc accaacaagg tgatcctcgg cctcacggcc ggggtcggag ccaccatcgc
     9001 ggcggcgctc gccgccaccg ccaccgacgg ggccgtgtcc gcctcggaac tcttcatcta
     9061 cccgttgccg ctcgtggcca cgttggcgat cgtgacgggc atcctgatgt tcaccgccga
     9121 ggcgcagcac ggcacgctgg ccgtgacgct cgccgcccgc ccgcccggcg cggtgggtca
     9181 ccgtcgtggc caagacggtc atggccgtcg ccgtgggtgc cacgtacggc gcagcggcaa
     9241 tggcggcagg gttcgccggg gcgcttgccg gtggcgccga gctcggcgac ggttcggcga
     9301 tcccggcacg cgcagcgtgg gcgctgctgt acatcgcgct cgccgcggtc atcggactgg
     9361 gcgtcggcat gatcgttcgt cacagcgccg gtgccatctc gggcctgttg atgtggtcgt
     9421 tcgtgatcga gaccctcttc gccccggcgc tgcccgacgg cgtgctgcac ttcctgccgt
     9481 tcagtgccgg ctaccggctc ctcgacgccg gcgcggactt caaggcgccg gtcgtcatcg
     9541 ccgacgagct gtcccggccg atgtacgccg tgatcttcgg tgggtacgcc ctggtcacac
     9601 tcgccgtcgg caccctgctc ctggcccggc gggacacgaa ctgacgaccg atcgttcggt
     9661 gttgcacatt gctacacttg ttgcagaccg gtacgcagga ggaagtagcg ccatggcgaa
     9721 gagcacgtcg gttccgtccc gtatcaccgc cgacgtggct gcagccgcgg cggcgatcgc
     9781 tcctggcgag caccgcacga tcaccgagca gatcaactat tgggcgcgaa tcggtatgca
     9841 ggtcgagcgc tcgggctcgc tggccagccg acgtgtgctc gccgtggctg ctggcgccga
     9901 gcagttctcc ggtctcgcga ccgaggaacg gctggcggcg cacgccctga tcgatgcggg
     9961 gattgcggag cgggcggcga gtgagcggtt cgggccggcg gcgagagccg ccggccagac
    10021 gaccgtctcg atcgaggacg gccacctcgt cgagatctcg cccggtggag cacgccgtcg
    10081 gttgtgagcc gcctcgacct gatcgtcggc cccaacgggg ccggccagac gacgctgtac
    10141 gagcgggtga tcgctcccgg tcgaccgggc ctgccgttcg tcaatgccga ccggattgcg
    10201 gcagaccgct tcggcgacga ggccgaagcc cgctcgtatg aggcagcgga gatcgccgag
    10261 gccgcacgag acgcactgat cgctgcacgg ctcgacttct gctccgagac cgtgttctcc
    10321 cattcgtcga aggtcgacct cgtcgccggc gccgtcgagg cgggctacga gatcgtgctc
    10381 cacgtggtga tgattccact cgagctgtcc gggcctcgcg tcgccgtccg cgtcgccgcc
    10441 ggagggcact cggtgcctca gggcaagctg gcggcgcgct atcggcgcct gtggcccaac
    10501 gtggcagcgg ccgtaccgct ctgccgccgc gccgtgttct gggacaactc gtcccgggac
    10561 ggcccggtcg aggtcgcggc gttccgcctg ggggttgccg acttcccgcc gcgttggccc
    10621 gactggactc ccgaggagct gcgtctcctc tgacgccggc tccggagcaa cagcagtcga
    10681 gccggatctc actgtcagga ccacctgcga tactgaaccc atgacgaaca ccagttcgta
    10741 tcgatcacaa ctcgccggcc aactccgaga cgccgcggtg cctctacgca ccctgctgag
    10801 cgatttgtcg ccggccgatg tgccgttgcc gtacgccgtc gacatcttgg acgcgttcat
    10861 cgaactcgaa cgcctcgccg cctccggcaa ggtcctcatc gcagcccgcg ccgccgaatc
    10921 cgaagcatgg cgacgagccg gcagcccatc tgcggccgcg tggttggcgt tgcggcaagg
    10981 caccgacacc ggccgggccc gcgccgatct cgaaacatcc caacaactcg gtgggctcac
    11041 gggcaccgcc gacgcggtcc gcaacggcga gttgtcaccc caacaggccg gtgccgtcgc
    11101 tgcgggggcg tcagtcaacc ccgctgcaga ggacgacctg ctcgagacgg cgaagtcgga
    11161 ttcgctccgg cacaccaaag aagaggcggc gcggcggcgc gctgaggtcg aaggggagga
    11221 atcgaaagtc gaacgcgaac gcaaggt
//
LOCUS       DAOSOA010000074        11210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5787_length_11210_cov_6.645719, whole genome shotgun sequence.
ACCESSION   DAOSOA010000074 DAOSOA010000000
VERSION     DAOSOA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5787_length_11210_cov_6.645719"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKY13_06660"
     CDS             <1..372
                     /locus_tag="PKY13_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOT79651.1"
                     /translation="ASATQDVPLWVVLAAHSAIALGTLSGGWRIVKTMGSKITRLQPV
                     GGFAAETGAALMLFFASAVGIPVSTTHTITGAIVGVGTQKRMSAVRWAVAGQVVWAWV
                     LTIPGAFAVGWVASVIIKALG"
     gene            418..603
                     /locus_tag="PKY13_06665"
     CDS             418..603
                     /locus_tag="PKY13_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79652.1"
                     /translation="MLLIIGAIAAGAMYPVVAPLDTADPSKPQHFGLTVMIVVIVYCV
                     LAILFTLDNISKRSRRR"
     gene            735..2033
                     /gene="hisS"
                     /locus_tag="PKY13_06670"
     CDS             735..2033
                     /gene="hisS"
                     /locus_tag="PKY13_06670"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT79653.1"
                     /translation="MAQPKYQSLKGMHDLLPPESEEWLEVVDRFRRQFGRSGYGYIQT
                     PILEDLAVFTRVGEGTDVVTKEMYDFVDKDGSTIALRPESTAGVARSFIQHRPLTPWK
                     VWYTSQHFRHENTQAGRYRQHHQLGAECFGSDHPDIDVELIVGLWDFYTQSRDDGGLG
                     LRRVRLQLNSLGERETRVRYQEMLRTYLESRLDDLHPTDRDKVQRNPMRVLDTKFAES
                     FPVVEEAPRLVDHLSESDRDRFERVRYGVLEAGIPFDIEPRLVRGLDYYTHTVFEIVS
                     DALESAQSTIGGGGRYDGLVEALGGPSTPGVGFGSGIERILLALRAEEVAPLASFALD
                     AFVVTFGGIHPDPRELMLGLRRHGIGVDRALNQHNPRKQMDRAKRVNPRFVIRLGKDA
                     DRPNVVEVADQRDDITWEVDLGPVVPEQAGRLAEEIHRRR"
     gene            2043..3833
                     /gene="aspS"
                     /locus_tag="PKY13_06675"
     CDS             2043..3833
                     /gene="aspS"
                     /locus_tag="PKY13_06675"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT79654.1"
                     /translation="MTDSTLLPRPDLLAHLPTHLRTNMCGELRPEHVGRTVSVCGWVA
                     SRRVHSEHLAFVDLRDYTGIVQCVVDGAADLRSEYVVRVTGTVVAREEQWLNPKLATG
                     EVELQDCTVEVLSAAEPPPFQLDDRRETDEGIRLRHRYLDLRRDQMQRNLRTRATVNS
                     AIRRAMEGQGFLEVETPMLIASTPEGARDFVVPSRLKPGSFYALPQSPQLFKQLCMVG
                     GIDRYYQIARCLRDEDLRADRQFEFMQLDAEMSFASQDDVLNAISTTVAAVVEAVTGE
                     TIGDIPRMTWLEAAERFGSDKPDVRFGLELIELTEVFASTGFNAFKAPCIKGIRVPGG
                     AEFTRSRLDELTDKAKRWGAKGLVWMRVKEAGELDSPVAKFLSDDEKAALVSATGAET
                     GDLLLIVADERPKTRHVLGLLRIDIGKPPVNEGGLNFLWVVDFPLFESVDEATGKPVS
                     AHHPFTMPHEDDFGLLDGSPQELLDVRSQAYDLVLNGWELGSGSVRIHRGDIQQRVFE
                     LLGISNEEAQLKFGFLLDAFRFGPPPHAGFAFGIDRLVALLVGEENIREVIAFPKTQS
                     GSDPLTQAPTDIDPAQLTELGLRLLPRAAE"
     gene            complement(3945..4334)
                     /locus_tag="PKY13_06680"
     CDS             complement(3945..4334)
                     /locus_tag="PKY13_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79655.1"
                     /translation="MAPRFDLVGIVVADMSASLAFYRRLGFDIPADADGEPHVEATLP
                     NGVRFAWDLRSVITSFDTAWTPSTGGPGITLAFICDSPADVDETYASLVASGAGAHHE
                     PWDAFWGQRYATVVDPDGNHIDLFANL"
     gene            complement(4444..>5020)
                     /locus_tag="PKY13_06685"
     CDS             complement(4444..>5020)
                     /locus_tag="PKY13_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79656.1"
                     /translation="PDAPLPRSFPTAEFPQPARAEAGADLPDGFPPAFPAAFPPMPAD
                     PSTIGSAPAPAAAAPVTPAVPDPEFAGRDAGFARTGREEPEPGDLRTLFKGISMPAGL
                     RPLGPLVPITASFVTEASPAEVHAGLEAEFERLECVANWVEPTVAQTERNGMRGIVTI
                     YPNPAALTDLDGTPLFPGVAPGHVVVKMLAL"
     assembly_gap    5021..5120
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5121..6210
                     /locus_tag="PKY13_06690"
     CDS             <5121..6210
                     /locus_tag="PKY13_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT79657.1"
                     /translation="GRVGAMSDLFASAAEDRLAGRAPLAERLRPRTLDDVLGQGHLTG
                     PGRPLRTMIDRKTLRSIILFGPAGTGKTTLARLITTELGHAFEHLSAVSASVKDIRSV
                     ADAAASRLGMSGTATVLFLDEIHRFTRSQQDALLPHVESGLVTLIGATTESPWATVNR
                     PLLSRTTVLELKPLDEATLGALADRGLALVHKELSPEARRMIVGSADGDGRRLLTAVE
                     MAADLAPAGVIDEDTMTAALGAGIGHYDRGDHYDAASAFIKHMRAGNVDQAVAWVNHM
                     LDGGEDPRFIARRMVIFASEDVGLADRSSLLIATAAANATEFVGMPEVRYSLTHATIV
                     LARAPKSREVGEYMAKAPTITLEPPPGA"
     gene            6221..6583
                     /locus_tag="PKY13_06695"
     CDS             6221..6583
                     /locus_tag="PKY13_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79658.1"
                     /translation="MSGTDVVLVALTVVCLLAVAALAVTVVQLRHVVGRLHRELEQFT
                     ERAVPAATDLSASAQLAAHQVDRLENLLDVASQVTDTMDQATAATVRVLSSPVIKTAA
                     IARGTRKAARRLKSAEGT"
     gene            6583..6867
                     /locus_tag="PKY13_06700"
     CDS             6583..6867
                     /locus_tag="PKY13_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79659.1"
                     /translation="MFKRVIWMGIGAAAGSAGTIWAERKVKTQIDRVSQSVADKATVQ
                     HVTGVARDRIVDVRDTVVAAVSEGRSAKQSAESEMRQNVTERWGRHRPAG"
     gene            6910..9510
                     /gene="alaS"
                     /locus_tag="PKY13_06705"
     CDS             6910..9510
                     /gene="alaS"
                     /locus_tag="PKY13_06705"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT79660.1"
                     /translation="MRANELRHAFTTFFAERGHTPVPSSGLIPLHPSAPMFTNSGMMQ
                     FVPIFLGEEQAPYDPPRAVTVQKCVRAGGKHNDLDAIGRSPRHLSFFEMLGNFSFGDY
                     FKEGAIPMAWEFVTEVLGIDGDRIWVTVHTSDDEAEDIWADQVGFPRERIQRLDKDNF
                     WEMGETGPCGPSSELFYDYGAEIGPDGGPANPEAEHRFVEIWNLVFPQYFRGADGQLS
                     DLPKKNIDTGAGMERILALMAGSPSLYAADVLSVLVDEAQRVTGHRLGESEITDVALR
                     LMADHTRTMTFLVSDGVIPSNEDRGYVLRRIIRRAIRFAYVTGVERLVLGEMIDRCIG
                     VMSDAYPELIGNRDLILSTIEREEAKFRQTLKTGSSLLDSELSALPAGGRLDGEVAFK
                     LHDTYGFPLEVTQEFTELGGYELDVAGFEAAMANQRANSKAGAKATGVATDAEAGEFR
                     TILDEHGQTTFVGREVDESPARVIATVGNTVVLDVTPFYAESGGQIGDTGSISWDGTT
                     LHVIDTNSALPGLVRHVIAAGDADGLAGLAPGTVVTATIDADRRNAIRRHHTATHILH
                     WALRSVLGTHVKQQGSWVGPDRLRFDFSHFEGLTPDQIVAIEDLANREILTNAPVSHE
                     EMTKDEAMKLGAIAFFGDKYGDTVRVLQAGPNSIEFCGGTHVQRGLGDIGPLKIVSET
                     SIGSNIRRLEAIAGLEPIKLIRSEERTLSEVAATLGVPVGDIAEGLRKRLDENKAQKA
                     EIAALRRQVAQASAGDLVDAAVDGVLVARIESETRDEVRDLAVSLRDRPGMKAVVLIS
                     SPGGKGVAVAAAVDPESGLNAGEIIADAVKAVGGGGGKGEHLATAGGRNADGIDEALQ
                     LARAALGVGS"
     gene            9584..10042
                     /gene="ruvX"
                     /locus_tag="PKY13_06710"
     CDS             9584..10042
                     /gene="ruvX"
                     /locus_tag="PKY13_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOT79661.1"
                     /translation="MTRALGLDLGSKRIGVALSTSDGVLATPYEVVQRIGDRAQEHRK
                     IAALAAEAEAEVIVVGVPYHLDGRLSPNAHAALAEAAELHDTIGLPVETYDERLTTVT
                     AQRSLNEMNLSPAARRRAIDQVAAAVMLQAWLDHRRALPDGEPEQADEDP"
     gene            10039..11196
                     /gene="mltG"
                     /locus_tag="PKY13_06715"
     CDS             10039..11196
                     /gene="mltG"
                     /locus_tag="PKY13_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT79662.1"
                     /translation="MSNHDGYDDGYDEYDDDGYSLYDDDDVVYDRVPRTSRRGSPIAV
                     TIVALFAVVGILVAGVLVWATRQMNPSGEQGPKVASIEIPAGSSFDDVADILEKEKVI
                     SSAFVMGWYAKFNDVKPVKAGNYVDFHTNSSMADAVEVLDAGPVPPQTKVLTIIPGTW
                     LEDAFVAINKAFPAISVDQLSLTLLSGSVKSKYHGDDPKASWEGFLLPETYQFEETAT
                     AQDILQRLVDEFDATLTELGYDNATATTGHSAKDLVIIASMIERETGDPEDERPKMAR
                     VIFNRLDAGTPTGIDATILYGLGRKGNEGGLTKTELETPGPYNSRLTPGLPPTAISLP
                     SEQSLDAAIHPAEGDWIYYVLKTQSPRAHVFTASYKEFQKAKEECQKNGFC"
BASE COUNT         1775 a         3848 c         3785 g         1702 t
ORIGIN      
        1 gcgtccgcca cccaggacgt cccgctctgg gtcgtgttgg cggcccactc ggcgatcgct
       61 ctcggcaccc tgtcgggcgg gtggcgcatc gtgaagacga tgggctcgaa gatcacccgg
      121 ctgcagcccg tgggcgggtt cgccgccgag accggcgcgg cgctcatgct gttcttcgct
      181 tcggcggtcg gcatcccggt gtcgacgacg cacacgatca ccggcgccat cgtcggcgtc
      241 ggcacgcaga agcggatgtc ggccgttcgt tgggcggtcg ccggccaggt ggtgtgggcg
      301 tgggtcctca ccatcccggg ggcattcgcc gtcggctggg tggcatccgt catcatcaag
      361 gcgctcgggt agcgccggta cggcacggga aggactgcga acgtggaccg tcgcatcatg
      421 ttgctgatca tcggggccat cgccgccggt gccatgtatc cggtcgtcgc gccgctcgac
      481 accgccgatc cctcgaagcc tcagcacttc ggcctgaccg tgatgatcgt ggtgatcgtc
      541 tactgcgtcc tggcgatcct gttcacgctc gacaacatca gcaagcggag ccgccgccgc
      601 tgagcccgtc ggggcggcga agctttgaac cgccgcgcac gtcctccgtg cgttccggtt
      661 caaacctccg cgtcggtggt cttcggcgct cggtcggcat cgtgagttga gactcgcggg
      721 tagcgttggc ggtcgtggca caacccaagt accagtccct caaggggatg cacgacctcc
      781 tcccaccgga gtccgaggag tggctggagg tcgtcgatcg gttccgccgc caattcggcc
      841 gctccggcta cggctacatc cagacgccga tcctcgagga cctggcggtg ttcacccgcg
      901 tgggcgaggg gaccgacgtc gtcacgaagg agatgtacga cttcgtcgac aaggacgggt
      961 ccaccatcgc gttgcggccg gagtccaccg ccggcgtggc ccgctccttc atccagcatc
     1021 ggccgttgac gccgtggaag gtctggtaca cctcgcagca cttccgtcac gagaacaccc
     1081 aggccggccg gtaccgccag caccaccagc tgggcgcgga gtgcttcgga tccgaccatc
     1141 ccgacatcga tgtcgagttg atcgtcggtc tctgggactt ctacacccag tctcgggacg
     1201 acggcgggtt gggtctacgg cgggtccgtc tgcagctcaa ctccctgggg gagcgcgaga
     1261 cccgcgtccg ctaccaggag atgcttcgca cctacctcga gtcgcggctc gacgacctgc
     1321 acccgaccga tcgcgacaag gtgcagcgca accccatgcg ggtgctcgac accaagttcg
     1381 ccgagagctt cccggtcgtg gaggaggcgc cgcgcctcgt cgaccatctc tcggagtcgg
     1441 accgcgaccg gttcgaacgg gtgcgttacg gcgtcctcga ggcggggatc ccgttcgaca
     1501 tcgaaccccg gctcgtccgt ggcctcgact actacacgca caccgtgttc gagatcgtga
     1561 gcgacgccct cgagagcgca cagtcgacca tcggcggcgg tggccgctac gacggcctgg
     1621 tcgaggcgct cggtggaccg tccacccccg gcgtcgggtt cggctcgggc atcgagcgca
     1681 tcctgctcgc gctgcgcgcc gaggaggtcg ccccgctggc gtccttcgcg ctcgacgcgt
     1741 tcgtcgtgac cttcggcggc atccaccccg acccgagaga gttgatgctt ggcctccgcc
     1801 gacacggcat cggggtcgac cgggcgctca accagcacaa cccccgcaag cagatggatc
     1861 gggccaagcg ggtgaaccca cgcttcgtca tccggttggg caaggacgcc gatcggccca
     1921 acgtggtcga ggtcgccgac cagcgcgatg acatcacctg ggaggtcgac ctcggcccgg
     1981 tggttcccga acaggcgggc cgcctggccg aggagatcca ccgacgccgg taggctcggc
     2041 tcgtgaccga ttcgaccttg ttgccgcgcc ccgacctcct cgcgcacctg cccacccacc
     2101 tccgcaccaa catgtgcggc gagctccgcc ccgagcacgt cggccggacg gtgtccgtct
     2161 gcgggtgggt tgcctctcgg cgcgtccact ccgagcacct cgcgttcgtc gacctgcgtg
     2221 actacacggg catcgtgcag tgcgtcgtcg acggcgcagc ggacctgcgc agcgagtacg
     2281 tggtgcgggt caccggcacc gtcgtggccc gcgaagagca gtggttgaac cccaagctcg
     2341 ccaccggcga ggtcgagctg caggactgca ccgtcgaggt gctctcggcg gccgagccgc
     2401 ccccgttcca gctggacgat cgtcgtgaga ccgacgaggg catccgcctg cgccaccgct
     2461 acctcgacct gcgccgcgac cagatgcagc gcaacctgcg cacacgggcg acggtcaact
     2521 cggcgattcg ccgggcgatg gagggccagg ggttcctcga ggtcgagacc ccgatgctca
     2581 tcgcatcgac gcccgagggc gcccgcgact tcgtggtccc cagccgcctc aagcccggtt
     2641 cgttctacgc gctgccgcaa tcgccgcagc tgttcaagca gctctgcatg gtcggtggca
     2701 tcgatcgcta ctaccagatc gcccgttgcc ttcgcgacga ggatctccgc gccgaccggc
     2761 agttcgagtt catgcagctc gatgccgaga tgagcttcgc gagccaggac gacgtgttga
     2821 acgccatctc gacgaccgtc gctgccgtgg tcgaggcggt gacgggcgag acgatcggcg
     2881 atatcccgcg catgacgtgg ctggaggccg ccgagcggtt cggctccgac aagcccgacg
     2941 tgcgcttcgg gctcgaactc atcgaactca ccgaggtgtt cgcgtcgacg gggttcaatg
     3001 cattcaaggc cccctgcatc aagggcatcc gggtgcccgg tggcgccgag ttcacccgga
     3061 gtcgcctcga cgagctcacc gacaaggcca agcgttgggg tgccaagggc ctggtctgga
     3121 tgcgcgtcaa ggaggcgggc gagctcgaca gccctgtcgc caagttcctg agcgacgacg
     3181 agaaggcggc gttggtctcc gcgaccggtg ctgagacggg cgacctgctc ctcatcgtgg
     3241 ccgacgagcg ccccaagacc cgccacgtgc tcggcctcct ccgcatcgac atcggcaagc
     3301 cgccggtcaa cgagggtggc ctcaacttct tgtgggtcgt cgacttcccg ctgttcgaat
     3361 cggtcgacga ggcgacgggt aagccggtgt cggcgcacca cccgttcacg atgccccacg
     3421 aggacgactt cggtctcctc gatggttcac cccaagagct gctcgacgtg cggtcgcagg
     3481 cctacgacct cgtcctcaac ggttgggagt tgggctcggg ctcggtccga atccaccgcg
     3541 gtgacatcca gcagcgcgtg ttcgagctgc tcggcatctc caacgaggag gcgcagctca
     3601 agttcgggtt cctgctcgac gcattccggt tcggaccgcc gccgcacgcc gggttcgcgt
     3661 tcggcatcga ccggctcgtc gccctgctgg tgggcgagga gaacatccgt gaggtcatcg
     3721 cattccccaa gacgcagtcg ggaagcgacc cgttgaccca ggcgccgacc gacatcgatc
     3781 cggcccagct caccgagctc ggcctgcgtc tcctgccccg ggcggccgag tagctcggtc
     3841 atcgcctcgg ccgccaacgc gtcggggggt tctgcgggta gttgtcgcca cctgatgagg
     3901 acaagtaccc gcgaaacgtg ggtggtgggc cgcgctcgct cgggttagag gttggcgaag
     3961 agatcgatgt ggttgccgtc ggggtcgacg acggtggcgt accgctggcc ccagaaggcg
     4021 tcccagggct cgtgatgggc accggcgccc gatgcgacga gcgacgcgta cgtctcgtcc
     4081 acgtcggcgg gggagtcgca gatgaaggcc agcgtgatgc cgggaccgcc ggtggacggg
     4141 gtccaagcag tgtcgaacga cgtgatcacc gagcggaggt cccacgcgaa gcggacgccg
     4201 ttcggcaggg tcgcctcgac gtgcggttcg ccatcggcat ccgcggggat gtcgaagccg
     4261 aggcgacggt agaaggcgag cgaggcggac atgtcggcga cgacgatgcc cacgaggtcg
     4321 aatcggggag ccattcgctc accgtaccgg ccgccacggt cgcgttcccg acgtggaacc
     4381 ccgatcctcc gggtttcccg tcgggagaag gggtagcggg tgagcgctgg gaggtcgccg
     4441 agttcagagt gcgagcatct tgaccacgac gtggccgggg gcgacgccgg ggaagagcgg
     4501 ggtgccgtcg agatcggtga gcgctgcggg gttcgggtag atcgtgacga tgccccgcat
     4561 gccgttgcgc tcggtctggg cgaccgtcgg ctcgacccag ttcgcgacgc attcgaggcg
     4621 ttcgaactcg gcctcgagcc cggcgtgcac ctcggcgggc gacgcctcgg tcacgaagct
     4681 ggcggtgatg ggcaccaacg gcccgagggg acgcaggccg gcaggcatcg agatgccctt
     4741 gaacaacgtg cgtaggtcgc cgggctccgg ctcctcgcgg ccggtccggg cgaacccggc
     4801 gtcgcggccg gcgaactccg ggtcgggaac ggcgggggtg accggcgcag cagcggccgg
     4861 cgccggcgca ctgccgatcg tcgacgggtc ggccggcatc ggagggaatg ccgccgggaa
     4921 agcgggcggg aacccgtccg ggaggtctgc tccagcctcg gcgcgagccg gctgggggaa
     4981 ctcggcggtg gggaacgaac gcggcagggg agcgtcgggc nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn gggtagggtc ggcgcgatgt cggacctgtt cgcctcggcc
     5161 gccgaggatc gcctggccgg tcgggcgccg ctcgccgaac gcctccggcc ccgcaccctc
     5221 gatgatgtcc tcggccaggg gcacctcacc gggccgggcc gtccgttgcg gaccatgatc
     5281 gaccgcaaga cgctgcgatc gatcatcctc ttcggtccgg cgggcaccgg aaagacgacg
     5341 ctcgcccgcc tgatcaccac cgagctcggc catgcgttcg aacacctgtc ggccgtcagc
     5401 gcgagcgtca aggacatccg ctcggtcgct gacgcggcgg cgtcgcggct cggtatgagc
     5461 ggcacggcca cggtgttgtt cctcgacgag atccaccggt tcacccggtc gcaacaggac
     5521 gcgctgcttc cccacgtgga gtcgggactc gtcaccctca tcggcgccac gaccgagagt
     5581 ccgtgggcca cggtgaaccg tccgctcctg tcgaggacga ccgtcctcga gctcaagccg
     5641 ctcgacgagg cgacgctcgg cgccctcgcc gaccggggtc tggcgctggt ccacaaggag
     5701 ttgtcccccg aagcgcgacg catgatcgtg ggttccgccg acggtgacgg gcgacgcctg
     5761 ctcaccgcag tcgagatggc ggcggacctt gcgccggcgg gcgtcatcga cgaggacacg
     5821 atgaccgccg cgctcggcgc ggggatcggc cactacgacc gcggcgacca ctacgacgcc
     5881 gcgtccgcct tcatcaagca catgcgggcg ggcaacgtcg accaggcggt ggcgtgggtg
     5941 aaccacatgc tcgacggtgg tgaggatccc cggttcatcg cccgtcgtat ggtgatcttc
     6001 gcgtccgagg acgtcggatt ggccgaccgg tcctcgctgc tcatcgccac cgcagcggcc
     6061 aacgccaccg agttcgtggg gatgcccgag gtccgctaca gcctcaccca cgccacgatc
     6121 gtgctcgccc gtgctcccaa gtcccgggag gtcggcgagt acatggccaa ggcgccgacc
     6181 atcaccttgg agcccccgcc cggggcctag cgttcccgcc atgtccggca ccgatgtcgt
     6241 cctggtcgca ctgaccgtcg tctgcctcct ggccgtcgct gcgctcgccg tgacggtggt
     6301 gcagctgcgt cacgtggtcg ggcggctcca ccgggagctc gagcagttca ccgaacgggc
     6361 ggtgccggca gcgaccgacc tgtcggcgtc ggcgcagctg gcggcccacc aggtcgaccg
     6421 gctcgagaac ctgctcgacg tggcgtcaca ggtgaccgac accatggacc aggcgacggc
     6481 cgctaccgtc cgggtcctgt cgagcccggt catcaagacc gcggccatcg cccgcggcac
     6541 ccgcaaggcg gcccgacgac tcaagagcgc tgaggggacc tgatgttcaa gcgagtgatc
     6601 tggatgggaa tcggcgccgc agccggctcg gcgggcacga tctgggccga acgcaaggtc
     6661 aagacgcaga tcgaccgcgt gagccagtcc gtcgccgaca aggcgaccgt gcagcacgtg
     6721 accggtgtcg cccgtgaccg catcgtcgat gtccgtgaca cggtcgtcgc tgcggtgagc
     6781 gagggtcgga gcgccaagca gtccgccgag tccgagatgc gtcagaacgt caccgaacga
     6841 tggggtcgcc accgacccgc cggctaacag gttcgtgtcc tcgccggccg ccggtaggat
     6901 cccgtcacca tgcgcgccaa cgaactccgc cacgcattca cgacgttctt cgccgagcgg
     6961 ggtcacacgc cggtcccgtc gtcgggtctg atcccgctcc acccgagcgc gccgatgttc
     7021 acgaactcgg ggatgatgca gttcgtcccc atcttcttgg gggaggagca ggccccctac
     7081 gacccgcccc gggccgtcac ggtgcagaag tgcgtgcgtg ccggtggcaa gcacaacgat
     7141 ctcgacgcca tcggtcgcag cccccggcat ctctcgttct tcgagatgct cggcaacttc
     7201 agcttcggcg actacttcaa agagggcgcc atcccgatgg cgtgggagtt cgtcaccgag
     7261 gtgctcggta tcgacggcga ccgtatctgg gtgaccgtcc acacgagcga cgacgaggcc
     7321 gaggacatct gggccgacca ggtgggcttt ccccgcgaac gcatccagcg cctcgacaag
     7381 gacaacttct gggagatggg cgagaccggc ccctgcgggc cgtcatcgga gctgttctac
     7441 gactacggcg ccgagatcgg tcccgacggt ggcccggcca accccgaggc cgagcaccga
     7501 ttcgtcgaga tctggaacct cgtgttcccc cagtacttcc gcggcgcgga cggtcagctg
     7561 agcgacctcc ccaagaagaa catcgacacc ggcgccggta tggagcgcat cctggcgctg
     7621 atggcgggca gtccgtcgct gtacgccgcc gacgtgttgt cggtgctcgt cgacgaggcg
     7681 cagcgggtca ccgggcatcg gctcggcgaa tccgagatca ccgatgtggc cctccgcctc
     7741 atggccgacc acacccgcac catgacgttc ctcgtgtccg acggggtcat tccgtccaac
     7801 gaggatcgcg gctacgtcct gcgccgcatc atccgccgtg ccatccgctt cgcgtacgtc
     7861 accggcgtcg agcggctggt gctcggcgag atgatcgacc gttgcatcgg ggtgatgagc
     7921 gacgcctacc ccgagctcat cggcaaccgg gacctgatct tgtcgacgat cgagcgggag
     7981 gaggcgaagt tccgccagac cctcaagacc ggttcgtcgc tcctcgacag cgagctgtcg
     8041 gcgctcccgg ccggcggtcg gctcgacggc gaggtcgcct tcaagctgca cgacacctac
     8101 ggcttccccc tcgaggtcac ccaggagttc accgagctcg gtggctacga gctcgacgtg
     8161 gccggcttcg aggccgccat ggccaaccag cgggccaact cgaaggctgg agcaaaggcc
     8221 accggtgtcg ccaccgacgc cgaagccggc gagttccgga cgatcctcga cgagcacggc
     8281 cagacgacgt tcgtcggccg cgaggtcgac gagtcgccgg cccgggtcat cgccacggtc
     8341 ggcaacacgg tcgtgctcga tgtcacgccc ttctatgccg aatccggcgg ccagatcggt
     8401 gacaccgggt cgatctcgtg ggacggcacc acgctccacg tgatcgacac caactccgcc
     8461 ctgcccgggc tggtgcgtca cgtcatcgcc gccggcgacg ccgacggcct cgccggcctc
     8521 gccccgggca cggtcgtcac cgccacgatc gatgccgatc ggcgcaacgc catccggcgc
     8581 caccacaccg ccacccacat cctccactgg gcgctgcgca gtgtgctcgg cacgcacgtc
     8641 aagcagcagg gttcgtgggt cggtcccgat cgtctgcgat tcgacttcag ccacttcgaa
     8701 ggcctgacgc ccgaccagat cgtcgcgatc gaggatctcg ccaaccgcga gatcctgacc
     8761 aacgccccgg tgtcgcacga ggagatgacg aaggacgagg cgatgaagct gggagccatc
     8821 gcgttcttcg gcgacaagta cggcgacacc gtgcgggtgc tgcaggccgg tccgaactcg
     8881 atcgagttct gcggcggcac ccacgtgcag cgcgggctcg gtgacatcgg tccgctcaag
     8941 atcgtgtccg agacgtcgat cggctcgaac atccggcgtc tcgaggccat cgccggcctc
     9001 gagcccatca agctcatccg gtccgaggag cgcacgctgt ccgaggtggc ggcgaccctc
     9061 ggggtcccgg tgggcgacat cgccgaaggc ctgcgcaagc gactcgacga gaacaaggcg
     9121 cagaaggccg agatcgccgc gctgcgccgc caggtcgccc aggccagcgc cggcgacctc
     9181 gtcgacgctg ccgtcgacgg tgtgctcgtc gcacggatcg agtcggagac ccgcgacgag
     9241 gtccgcgacc tcgccgtctc gctccgcgat cgccccggaa tgaaggccgt ggtgctcatc
     9301 agctcgccgg gtggcaaagg ggtcgccgtc gccgcggcgg tcgatcccga gagtgggctc
     9361 aacgccggcg agatcatcgc cgacgccgtg aaggcggtcg gcggcggtgg tggcaagggc
     9421 gagcacctgg ccaccgccgg tggtcgcaac gccgacggca tcgacgaggc cctgcaactc
     9481 gcccgagcgg cgctgggagt cgggtcctga caccgaaatg tgtcgcgagg tgtgcggttt
     9541 cgcgattgcg ccgccgcaca tttggtgagc ggagcgggtc gcgatgactc gcgcgcttgg
     9601 tctcgacctc gggtcgaagc ggatcggggt cgcgttgtcg acgagtgacg gcgtcctggc
     9661 gacgccgtac gaggtggtgc agcgaatcgg cgaccgcgcc caggagcacc gcaagatcgc
     9721 cgcgctcgct gccgaggcgg aggccgaggt gatcgtcgtc ggcgttccat accatctgga
     9781 cggacgtctc agtcccaacg cccacgcggc cctcgccgag gcggccgagc tgcatgacac
     9841 catcgggctc cccgtcgaga cctacgatga gcgattgacg accgtgaccg cccagaggtc
     9901 gctgaacgag atgaacctga gccccgctgc ccggcggcgg gccatcgacc aggtcgctgc
     9961 ggcggtgatg ctgcaggcct ggctcgatca ccgccgggca ctccccgacg gcgaaccaga
    10021 acaggcagac gaagatcctt gagcaatcac gacggctacg acgacggata cgacgagtac
    10081 gacgacgacg gctactcgct ctacgacgat gacgacgtcg tgtacgaccg ggttccccgc
    10141 acgtcgcggc gtggttcacc catcgccgtc accatcgtcg ccctgttcgc cgtcgtcggc
    10201 atcctcgtcg ccggtgtcct ggtgtgggcg acccgccaga tgaacccgtc gggcgagcag
    10261 ggcccgaagg tcgcgtcgat cgagatccct gcgggctcga gcttcgacga cgtggccgac
    10321 atcctggaga aggagaaggt catctcgagt gccttcgtga tgggctggta cgcgaagttc
    10381 aacgacgtga agccggtgaa ggccggcaac tacgtcgact tccacaccaa ctcgtccatg
    10441 gcggacgcgg tcgaggtgct cgacgccgga ccggtcccgc cgcagacgaa ggtgctcacc
    10501 atcattcccg gcacgtggct cgaggacgcg ttcgtcgcca tcaacaaggc gttccccgcg
    10561 atctcggtcg accagctgag cctgacgctc ctgtcggggt cggtgaagtc caagtaccac
    10621 ggcgacgatc cgaaggcatc gtgggaaggc ttcctgctgc ccgagaccta ccagttcgag
    10681 gagaccgcca cggcgcagga catcctgcag cgcttggtcg acgagttcga cgccaccctc
    10741 accgaactcg ggtacgacaa cgcgaccgcg accacggggc actcggcgaa ggatctcgtc
    10801 atcatcgcct cgatgatcga acgcgaaacc ggcgaccccg aggacgagcg tccgaagatg
    10861 gcccgggtga tcttcaaccg gctcgacgcc ggcacgccca cgggcatcga cgccacgatc
    10921 ctgtacggcc tcggtcggaa gggcaacgag ggcgggctga ccaagacgga gctcgagacg
    10981 cccggcccct acaactcccg cctcaccccc ggcctgccac ccacggcgat ctcgctgccg
    11041 tccgagcagt cgctcgacgc cgcgatccac cctgccgagg gcgactggat ctactacgtg
    11101 ctcaagacgc agtcgccgcg ggcccacgtg ttcacggcga gctacaagga gttccagaag
    11161 gcgaaggaag agtgccagaa gaacgggttc tgttgacccc tcggggtgag
//
LOCUS       DAOSOA010000075        11144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5834_length_11144_cov_5.253044, whole genome shotgun sequence.
ACCESSION   DAOSOA010000075 DAOSOA010000000
VERSION     DAOSOA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5834_length_11144_cov_5.253044"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1231)
                     /locus_tag="PKY13_06720"
     CDS             complement(<1..>1231)
                     /locus_tag="PKY13_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT79663.1"
                     /translation="PRLDQLGELLAWVEWSHPDMPWDASELWFGRLDLGGLEPALVGA
                     RHLAGGRDALTTGDESVVQPEWDQDNRLWFCSDRTDWWNLYHYGIPGPPTGRPIPVAA
                     GTYEVALPAWVFGESRYAFLSDNRVVFAYRSDGVDHLGVYDTIADRVDRVSVPCTSIR
                     QVRAWQTTVIFVGASFTADAAVVATIVGRGGASSGLEAMRPPGAEPGLSSSQLSVGQP
                     ITFPTDGGIAHAIFYPPTNPDFVAPEGERPPLIVMIHGGPTSAAAPELNLRTQYWTSR
                     GFAVVDVNHRGSTGFGRRFRNELRGQWGVVDVADCIAAARFLASAGRGDGERLIIRGG
                     SAGGYTTLAALTTSDAFSAGCSLYGIADLVLLTVDDHKFESRYTASLVAPYPEGAEVY
                     RERSPIHHLERLTSPIIM"
     assembly_gap    1232..1331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1332..1513)
                     /locus_tag="PKY13_06725"
     CDS             complement(<1332..1513)
                     /locus_tag="PKY13_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79664.1"
                     /translation="MGSSSRLGEVAISDDAIWWSEGRPSEAGRTQIVRRSPNGELAEV
                     LPTGTSAQSKVHEYGGG"
     gene            complement(1567..2787)
                     /locus_tag="PKY13_06730"
     CDS             complement(1567..2787)
                     /locus_tag="PKY13_06730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT79665.1"
                     /translation="MTSSPASPKPRVSRRVGSIAPSATLAVDAKAKALKAQGEPVIGF
                     GAGEPDFPTPDYIVDAAVAAARDSANHKYTPAGGLPALQEAIATKTARDSGFEVAPSQ
                     VLVTNGGKHALYNAFATLLDPGDEVLLPAPYWTTYPESIRLAGGAPVELPTHEATGYQ
                     VTIEQLDAALTPRTKVLVFVSPSNPTGAVYPPAAVEAIGRWAVEHGVWVVTDEIYEHL
                     VFGDAEFSSMPTLVPELADTCVVVNGVAKTYAMTGWRVGWMIGPSDVIKAAGNLQSHA
                     TSNVANVSQRAALAAVSGDLSAVAMMREAFDRRRLVMHRMLNDIDGVTCIEPEGAFYA
                     FPNFAGLLHRPIGGREAATTLELADIILEVAKVAIVPGEAFAAPGYARLSFAVADDDI
                     VEGIARIADLVAKG"
     gene            2948..4069
                     /gene="serC"
                     /locus_tag="PKY13_06735"
     CDS             2948..4069
                     /gene="serC"
                     /locus_tag="PKY13_06735"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOT79666.1"
                     /translation="MTETITIPASLLPADGRFGCGPSKVRPEAIAALADAAPTYLGTS
                     HRQAAVLFEVGALRNNIAELFSLPDGYEVLLGNGGSTLFWDVATFGLIRERSQHLVFG
                     EFSSKFFSASKSAPHLGQPSKIETDPGTHPLSVAEDGIDLYAMTHNETSTGVMMPLTR
                     PEGIDDDALVVVDATSGAGGLRVDMSQVDVYYFAPQKCFASDGGLWIACCSPRAIARI
                     EEIAASDRWRPASLDLGIALESSRKDQTYNTPALATIFLANQQLRWVLDNGGLEFAAG
                     RSDQSSSHLYGWADAHELATPFVTVEAERSRVVGTIDFADSVDAARLAAVLRHNGILD
                     VEPYRKLGRNQLRVAMFPAVDPSDVAALTACVDYVLERL"
     gene            4352..4897
                     /locus_tag="PKY13_06740"
     CDS             4352..4897
                     /locus_tag="PKY13_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79667.1"
                     /translation="MANGRILVHAADRPAPSSNTAHVRRPLIVTPDRGSPRALADGEV
                     WGPDAEIFTVSITGAHVWELDVVNFRDRAPEPGLPGDLPTVTQRVDLPDDLWTVLRAY
                     ALPVQRGERYPATHEQVARTVHWTKTTVRRRLERIYDEFYLRRMELPDVLDMRIQVVE
                     AAMSHGLIPRDLGDNGDAASS"
     gene            4927..6846
                     /locus_tag="PKY13_06745"
     CDS             4927..6846
                     /locus_tag="PKY13_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT79668.1"
                     /translation="MDERLWTGPADAPRRYQVDSSGGGPLHEPGGGVEGRVYRAVIVD
                     PGAPDDGDEVALKLLLERRPEELAEIDERLRLLTEVDGADLMAVRGTFVGTALTDEEH
                     PDPADFDVCYVVSDWIEGAPLGQAAEGQDQRWILGVLAGIARSVDHLHRTTGPGAPDG
                     MVHRDVKPSNIRVRADGSPVLVDYGLTRAVTEDASVVGTPGWLAPEVAAGSPGGQAAD
                     VYGVGAIGHDLLVGAPPRRDGVPAAARRINAALDDVPLAHEIGHHIATLLATDPDERP
                     TDLVAWAADLERLLDGQSLAGRSSAHRRWAVGSGLVAAAVVAVVVAATWSGDARDRTA
                     AAVEGTTVTADGASADGALADTGTTTSTATTSTVAGWVCSNDIDLPAGPVADAIRTAY
                     ADHNDLCTGAGDPLGDALVLPLLDRAGEPVAALIASPTTPAVYLSTSQYASYRQIVGR
                     SGDASAPLLGGYPTGVRVDPATGLGIVDLSLGGMVIGPTEGAQGFWLPQQLRGMWEER
                     GGLTGELGAPTSPVFISGTMIRIEFERGYIDAPIKDGLGGGAQLVPLTESTMAVHPVD
                     PAAELAALGDVADRVLRQSNGTAWYVDADLVRHWIPDGPTWGCLQANEKVVPVDAPGY
                     AVAALEVGDRARCRR"
     gene            6919..8280
                     /locus_tag="PKY13_06750"
     CDS             6919..8280
                     /locus_tag="PKY13_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOT79669.1"
                     /translation="MSAPLRYLLTDDEMPTAWYNLVPDLPAPPPPALHPGTLEPAGPD
                     DFAPLFPMDLIMQEVTSDRFVDIPGEVMDIYRLWRATPLHRARRLEQALGTPARIYYK
                     YEGTSPAGSHKPNTAVPQAYYNAKAGIRKLTTETGAGQWGTALAFACQMFGIECEIWQ
                     VRASYDQKPYRRAMMEAFGGTVHPSPSDVTEYGRKLLEQDPDQPGSLGIAISEAVEVA
                     AQDPEIRYALGSVLNHVLMHQTIIGEEALLQMAKAGDTPDLIIGCTGGGSNFGGLTFP
                     FLREKLAGNMNPTIRAVEPAACPSLTRGVYAYDFGDTAGMTPLMKMHTLGHDFIPDPI
                     HAGGLRYHGMSPLISHVYELGLIEAEAIPQSDCFAAGVQFARTEGIVPAPEPTHALAA
                     TIAEALRCKETGEEKVILTALCGHGMLDMAAYDRYFAGDIVDYDYPADKVADALTRLP
                     QVD"
     gene            8401..10152
                     /locus_tag="PKY13_06755"
     CDS             8401..10152
                     /locus_tag="PKY13_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOT79670.1"
                     /translation="MTTFTELQAQMTGPGGPFETQTEVIDTGLGEVEMTMYAQRFGDL
                     RQVAQFAALAHGDKEFIVYGDRRITFAEFFAQANTISAALLAAGVVKGDRVAVLSQNN
                     PEWCLTFWATVNIGAVLVGLNGWWTTDEILYGLEDSGAKVLVVDSKRFTRITTEINNI
                     DTLERVYLVDVDPGESGSTSAKVHAFSELLASSDGPAPDPTVELDEGDHAVIFYTSGT
                     TGRPKGAISTHRSMIANLQNTVFLITATTAIINEKSAAAGGEPPATPTQPVALFTSPL
                     FHVSGCHSTLVVGSLGGLKLVMIKGKFTPEGALQLISDERVTTWSTVPTMIWRTCEHP
                     DRHEYDTSTVTAVAFGGSPSADELQRRIRETFPNVKGTSNAYGLTETSSVATVLHGDA
                     ALTKADSVGLPVPTVRIAISDGRGGLAEPGATGEVLIGGPVLMAGYWNKPEATAKAIQ
                     DGWLYTGDVGHIDEDGYLFITDRAKDMIIRGGENIYCVEIENCLVEHEAIADAAIVGV
                     PHEGLGEEVKAFIELAPGASLTADEVQGWVAKHLAPFKVPTYVEFTDGKLPRNASGKL
                     LKNVLRGEGDVSFQETM"
     gene            10175..10426
                     /locus_tag="PKY13_06760"
     CDS             10175..10426
                     /locus_tag="PKY13_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79671.1"
                     /translation="MSDAPKAPPVPDGTYDVFIVDAVPHPDRPESVVKVEVTIVSGEF
                     KGEVLNLSANGISGSDIDLMGMPAALTVTDGVPVLTVEP"
     gene            10526..>11144
                     /locus_tag="PKY13_06765"
     CDS             10526..>11144
                     /locus_tag="PKY13_06765"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HOT79672.1"
                     /translation="MDCTKCGGRGEYYGIGSTGRFRDEPMMRDCDRCGRTGIEPGTNG
                     ATATGSAPAGGCLSMLFGWLAGWLARIVLALVGLAILGAIVFGFSWWKSRNHGLVEAR
                     PSSEVTTEGYAIGTCLNDPFGAALPVDCGQPHEAEVYRILEAKDPETEAYPGNGRLDD
                     FAAATCPEHFEDYVGATPADADLSIGSRGPTETEWRIGSRRVLCFA"
BASE COUNT         1735 a         4000 c         3744 g         1565 t
ORIGIN      
        1 acatgatgat cggcgacgtc aggcgctcga gatggtggat cggcgaccgc tcccgataca
       61 cctccgcgcc ctcgggatac ggcgcgacga ggcttgcggt gtagcgcgac tcgaacttgt
      121 ggtcgtcgac ggtgaggagc accaggtcgg cgatgccgta caggctgcac cctgcgctga
      181 aggcatcgga cgtcgtgagc gccgccaacg tcgtgtagcc gccggcgctg ccgccacgga
      241 tgatgaggcg ctcgccgtcg ccacggccgg ccgacgccag gaaccgtgcc gccgcgatgc
      301 agtcggcgac gtcgaccact ccccactggc cccgcagctc gttgcggaac cgacgaccga
      361 acccggtgct cccacggtgg ttcacgtcga ccacggcgaa cccacgcgac gtccagtact
      421 gcgttcggag gttgagctct ggggccgccg ccgacgtcgg accaccgtgg atcatcacga
      481 tcagcggtgg ccgctccccc tcgggtgcaa cgaagtcggg gttcgtcggc gggtagaaga
      541 tcgcgtgggc aatgccgccg tcggtcggga acgtgatcgg ctggccgacc gacaactgcg
      601 acgagctgag accgggctcc gcaccgggag gacgcatcgc ctcgaggccg gagctcgcgc
      661 caccacgccc gacgatcgtg gccacgacgg ccgcgtcggc cgtgaacgac gctcccacga
      721 agatcacggt cgtctgccat gcccgcacct ggcggatcga ggtgcacgga acgctgaccc
      781 ggtcgacgcg gtcggcgatc gtgtcgtaca cgccgaggtg gtcgacaccg tcggagcggt
      841 aggcgaacac gacccggttg tcgctgagga aggcgtaccg cgactcgccg aacacccacg
      901 ccgggagcgc gacctcgtag gtcccggcgg caacggggat cgggcgaccc gtcggagggc
      961 ccgggatgcc gtagtggtag aggttccacc agtcagtccg gtccgaacag aaccacagcc
     1021 ggttgtcctg gtcccactcg ggctgcacga cggactcgtc gccggtcgtg agcgcgtcgc
     1081 gaccaccggc gaggtgccgg gccccgacca gcgccggctc gaggccaccg aggtcgaggc
     1141 ggccgaacca cagctcggac gcgtcccacg gcatgtcggg gtgcgaccac tcgacccagg
     1201 cgagcagctc gccgagctgg tcgaggcgcg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nccgccgccg tactcgtgca ccttcgactg cgccgacgtg cccgtcggga
     1381 gcacctcggc caactcaccg ttggggctgc gccgcacgat ctgggtgcgt cccgcctcgc
     1441 tggggcgccc ctccgaccac cagatggcgt cgtcgctgat cgccacctcg ccgagccgcg
     1501 acgaactgcc catcacgcgc tcggcgctga tgggcgaacg ccacgacccg tagggcgcga
     1561 cctcggtcaa cccttggcca cgaggtcggc gatgcgagcg atgccctcga cgatgtcgtc
     1621 gtcagcgacg gcgaagctca gccgggcgta ccccggcgcg gcgaacgcct cgccggggac
     1681 gatcgccacc ttggcgacct cgaggatgat gtcggccagc tcgagcgtcg tggccgcctc
     1741 acgtccaccg atcgggcggt gcagcagccc ggcgaagttc gggaacgcgt agaacgcgcc
     1801 ctcgggctcg atacaggtga caccgtcgat gtcgttgagc atccggtgca tgacgaggcg
     1861 gcggcggtcg aacgcctccc gcatcatcgc cacggcggac aggtcgcccg acacggccgc
     1921 gagcgcggcg cgctggctga cgttggcgac gttggacgtt gcgtggctct ggaggttgcc
     1981 cgccgccttg atgacgtcgg acgggccgat catccacccg acgcgccagc cggtcatcgc
     2041 ataggtcttg gcgacgccgt tgacgacgac gcaggtgtcg gcgagctcgg ggaccaacgt
     2101 cggcatcgag ctgaactcgg cgtcgccgaa cacgaggtgt tcgtagatct cgtcggtgac
     2161 gacccagacg ccgtgctcga cggcccagcg gccgatcgcc tcgacggccg caggcgggta
     2221 caccgcacct gtcgggttgg acggcgacac gaacaccagc accttcgtcc gcggcgtcag
     2281 cgcagcatcg agctgttcga tcgtgacctg gtagccggtc gcctcgtggg tcgggagctc
     2341 gacgggcgcg ccgcccgcca gccggatcga ctcggggtac gtggtccagt acggcgccgg
     2401 cagcagcacc tcgtcgcccg gatcgagcag cgtggcgaaa gcgttgtaga gcgcatgctt
     2461 gccgccgttc gtgacgagca cctggctcgg cgccacctcg aagcccgagt cgcgggcggt
     2521 cttggtggcg atcgcctcct gcagggccgg aaggccaccg gcgggggtgt acttgtggtt
     2581 ggccgagtcg cgggcggcgg cgaccgcagc gtcgacgatg tagtccgggg tcgggaagtc
     2641 gggctcaccg gcgccgaagc cgatcaccgg ctcgccctgg gccttcagcg ccttggcctt
     2701 ggcatcgacc gccagcgtgg cggagggggc gatggacccg acgcgacggg agacgcgggg
     2761 cttgggggag gctggggagg aggtcacggc cccgattttc ggcccccggg gcggcgaagt
     2821 cgaatcggtt tcgggtcgcc tcgatgctcc ggagcgccgc atggggcccg aacgcaccgc
     2881 ggcgctccag gtgaacttcc cgatatcgat tagctggcac cctccggccc ttgggaaact
     2941 ggtcccgatg accgagacga tcacgatccc cgcttccctg ctccccgccg acggacgctt
     3001 cggctgcggc ccctccaagg tgcggcccga ggcgatcgcc gccctcgccg acgccgctcc
     3061 cacctacctg ggcacgagcc accgccaggc ggccgtcctg ttcgaggtgg gcgcgctccg
     3121 caacaacatc gccgaactgt tcagcctgcc cgacggctac gaggtgctgc tcggcaacgg
     3181 tggctcgacg ctgttctggg acgtcgccac gttcggtctg atccgcgagc gcagccagca
     3241 cctggtgttc ggcgagttct cgtccaagtt cttctccgcc tccaagtcgg cgccgcacct
     3301 cgggcagccc tcgaagatcg agaccgaccc cggcacccac ccgctctcgg tcgccgagga
     3361 cggcatcgac ctctacgcga tgacccacaa cgagacgtcg acgggcgtca tgatgccgct
     3421 cacgcggccc gagggcatcg acgacgacgc cctcgtcgtc gtcgacgcca cgtcgggcgc
     3481 cggcggtctc cgcgtcgaca tgagccaggt cgacgtctac tacttcgccc cccagaagtg
     3541 cttcgcctcc gacggtggtc tctggatcgc ctgctgctca ccgagggcca tcgcccgcat
     3601 cgaggagatc gccgcctccg accgctggcg gccggccagc ctcgacctcg gcatcgcgct
     3661 cgagagcagc cgcaaggacc agacgtacaa cacgccggcc ctcgccacga tcttcctcgc
     3721 caaccagcag ctgcggtggg tgctcgacaa cggcggcctc gagttcgccg ccggccgctc
     3781 cgaccagtcg tcgtcacacc tgtacggctg ggccgacgcc cacgaactgg cgactccgtt
     3841 cgtcacggtc gaggccgaac gcagccgcgt cgtcggcacg atcgacttcg cggactccgt
     3901 cgacgccgcc cgcctcgccg cggtgcttcg ccacaacggc atcctcgacg tcgagccgta
     3961 ccgcaagctg ggccgcaacc agctgcgcgt cgccatgttc ccggccgtcg acccgtccga
     4021 cgtcgctgcc ctgacggcgt gcgtcgacta cgtgctcgag cgcctctgac gtttcggggg
     4081 agaccggcag ggctgctcaa cctccgcccg acccgagacc agaaccgggt gatcgtggca
     4141 agctgccaca gatggcgctc acgatggatc tcgtgcaggg ggtcgttcgc ggccgcgtga
     4201 gccgccgcac gatgcccgac ggcgaccgct ccgagtttcc cctctccgac ggggatgtcg
     4261 ttgcgatcgg caggggcgcc gagtgcggca tccgattcgg tcacgcgccc gtccaggacc
     4321 gtgaggtgcc cgccactgcg gatggctggt cgtggccaac ggccgcatcc tggtccacgc
     4381 tgccgaccga ccggccccgt cgagcaacac ggcccacgtc cggcggccgt tgatcgtgac
     4441 ccccgaccgc gggtccccca gggcgctcgc cgatggcgag gtgtggggcc ctgacgccga
     4501 gatcttcacc gtctcgatca cgggcgccca cgtgtgggaa ctcgacgtcg tcaacttccg
     4561 cgaccgcgca ccggagcccg gactgcccgg tgacctcccg acggtgacgc aacgcgtcga
     4621 cctgcccgac gacctctgga cggtgctgcg ggcgtatgcg ttgccggtgc aacgcggtga
     4681 gcgctacccg gccacccacg agcaggtggc gcgaacggtg cactggacga agacgaccgt
     4741 ccgccgacga ctcgaacgga tctacgacga gttctatctc cggcgtatgg aactgcccga
     4801 tgtgctcgac atgcgcatcc aggtcgtcga ggcggcgatg agccacgggc tcatccctcg
     4861 agacctcggc gacaacggcg acgcggcgtc gagctgaacc gggcgactgc gggcggggga
     4921 cggaacgtgg acgagcggtt gtggacgggc ccggcggatg cgccgcggcg atatcaggtc
     4981 gactcgtcgg gaggtgggcc gctgcacgag cccggtggcg gtgtcgaggg ccgggtctac
     5041 cgcgccgtga tcgtcgatcc cggggcgccc gacgacggcg acgaggtcgc gctcaaactg
     5101 ctgctcgagc gtcgccccga ggagctcgcc gagatcgacg agcgtctccg gctcctgacc
     5161 gaggtcgacg gagccgacct catggcagtg cgcggcacct tcgtcggcac ggcgctcacc
     5221 gacgaggagc accccgatcc cgccgatttc gacgtctgct acgtcgtgtc ggactggatc
     5281 gagggagccc cgctcgggca ggcggcggag ggacaggacc aacggtggat cctcggggtg
     5341 cttgccggca tcgcccgatc ggtcgatcat ctgcaccgga ccaccggccc cggcgccccc
     5401 gatggcatgg tgcaccgcga cgtgaagccg tcgaacatcc gggtgcgggc cgacggctcg
     5461 ccggtgctgg tcgactacgg cctcacgcgg gccgtgaccg aggatgcgtc ggtcgtcggc
     5521 acacccggct ggctcgcgcc cgaggtcgcc gccgggagcc ctggtggcca ggcagccgac
     5581 gtgtacgggg tcggtgccat cggccacgac ctcctggtcg gcgcaccacc gcgccgcgac
     5641 ggggtgcccg ctgcggcacg acgaatcaac gcggcactcg acgacgtgcc gctcgctcac
     5701 gagatcgggc accacatcgc cacgctgctc gcgaccgacc ccgacgaacg cccaaccgac
     5761 ctcgtcgcct gggcagccga cctcgagcgg ttgctcgacg ggcaatcgct cgccggtcgg
     5821 agctccgccc accgacggtg ggccgtcgga tccggactgg tcgccgctgc ggtcgtcgcc
     5881 gtcgtggtcg ccgccacctg gtcgggcgac gcacgcgacc gaacggcggc ggccgtcgag
     5941 gggacgacgg tcacggccga cggcgcgtcc gccgacggcg cgttggccga caccgggacc
     6001 acgacctcga ccgccacgac gagcaccgtc gcgggctggg tctgttccaa cgacatcgac
     6061 ctccccgccg ggccggtcgc cgacgccatc cggaccgcgt acgccgacca caacgacctg
     6121 tgcaccgggg ccggggaccc gctcggcgac gcgctcgtcc tgccgctgct cgaccgcgcc
     6181 ggagaaccgg tggctgcgct catcgcatcg ccgaccaccc cggcggtgta cctctccacg
     6241 tcccagtacg cgtcgtaccg ccagatcgtc ggtcgcagcg gcgacgcctc cgctcccctc
     6301 ctcggcggct acccaaccgg ggtacgcgtc gacccggcga cggggctcgg catcgtcgac
     6361 ctgagcctcg gcggcatggt catcggaccg accgaaggcg cgcaggggtt ctggttgcct
     6421 cagcagctcc gcggcatgtg ggaggagcgc ggcgggctca cgggcgagct cggagccccc
     6481 acgtccccgg tgttcatcag cggcacgatg atccgcatcg agttcgaacg cgggtacatc
     6541 gacgcgccga tcaaggacgg gctgggcggc ggggcccaac tcgtgcccct caccgagtcg
     6601 acgatggcgg tccacccggt cgacccggcc gccgaactgg ccgcgctcgg cgatgtcgcc
     6661 gaccgggtcc tgcggcagtc caacggaacg gcgtggtacg tcgatgccga cctggtgcgg
     6721 cactggatcc ccgacggccc gacatggggc tgtctccagg ccaacgagaa ggtcgttccc
     6781 gtcgatgcgc cgggatacgc cgttgcggcc ctcgaggtcg gtgaccgggc gcgctgtcgg
     6841 cgctgacgct cgaccgaggc ctacgatcgc ccgatcggcc ggctgaccga ggcccgccgc
     6901 gaccatcagg gagttctgtt gtccgcaccg cttcgctacc tgctgaccga cgacgagatg
     6961 ccgacagcct ggtacaacct cgtgcccgac ctgcccgcac cacccccgcc ggcactccac
     7021 cccggcacgc tcgagccggc cgggcccgac gacttcgctc ccctgttccc gatggacctc
     7081 atcatgcagg aagtcacgag cgatcggttc gtcgacattc cgggcgaggt catggacatc
     7141 taccgcctgt ggcgcgcgac cccgctgcac cgcgctcgtc gtctggagca ggccctcggc
     7201 accccggcga ggatctacta caagtacgag ggcaccagcc ccgccgggtc gcacaagccc
     7261 aacaccgcgg tgccgcaggc gtactacaac gccaaggccg gcatccggaa gctcaccacc
     7321 gagaccggcg ccggccagtg gggcacggcg ctcgccttcg cgtgccagat gttcggcatc
     7381 gagtgcgaga tctggcaggt ccgggcgagc tacgaccaga agccgtaccg ccgggcgatg
     7441 atggaggcgt tcggcggcac ggtgcacccg tcgccgtccg acgtcaccga gtacgggcgc
     7501 aagctcctcg aacaggaccc cgaccagccc ggctccctcg gtatcgcgat ctccgaggcc
     7561 gtcgaggtcg cggcccagga cccggagatc cgctacgccc tcggctcggt cctcaaccac
     7621 gtgctgatgc accagacgat catcggtgag gaagcgctgc tgcagatggc gaaggccggc
     7681 gacacgcccg acctcatcat cggctgcacc ggcggcggct cgaacttcgg cgggctcacg
     7741 ttcccgttcc tccgcgagaa gttggccggc aacatgaacc cgacgatccg tgccgtcgag
     7801 cccgcggcct gtccgtcact cacccgcggt gtctacgcct acgacttcgg cgacacggcc
     7861 ggcatgacgc cgctcatgaa gatgcacacg ctcggccacg acttcatccc cgaccccatc
     7921 cacgccggcg gcctccgtta ccacgggatg agcccactca tcagccacgt gtacgaactg
     7981 gggctgatcg aggccgaggc gatcccgcag tcggactgct tcgccgccgg ggtccagttc
     8041 gcccgcaccg agggcatcgt cccggcaccc gagccgaccc atgcgctcgc cgccacgatc
     8101 gccgaggccc tccgctgcaa ggagaccggc gaggagaagg tcatcctcac ggcgctgtgc
     8161 ggccacggca tgttggatat ggcggcctac gaccggtact tcgccggcga catcgtcgac
     8221 tacgactacc ccgccgacaa ggtcgccgac gccttgaccc gcctccccca ggtcgactga
     8281 cgcccgaccc ctcggggttt tgtactgcga caacagccgt cagtggcgcg tttcgcagtg
     8341 cagaaccccg ggggacggtt ccgcagcgcg tgacgggcgt cacgctgcca agatgacgcc
     8401 atgaccacgt tcacggagct gcaggcccag atgaccggtc cggggggacc cttcgagacg
     8461 cagaccgagg tgatcgacac cggcctcggc gaggtcgaga tgacgatgta cgcccagcgg
     8521 ttcggcgacc tgcggcaagt cgcgcagttc gccgccctgg cccacggcga caaggagttc
     8581 atcgtctacg gcgatcgccg gatcacgttc gccgagttct tcgcccaggc caacacgatc
     8641 tccgcagcgc tgttggccgc cggggtcgtg aagggcgatc gagtcgccgt gctctcgcag
     8701 aacaaccccg agtggtgcct gacgttctgg gcgaccgtca acatcggcgc cgtgctcgtc
     8761 ggcctcaacg gctggtggac gaccgacgag atcctctacg gcctcgagga ctcgggcgcc
     8821 aaggtgctcg tcgtcgactc gaagcggttc acgcgcatca cgaccgagat caacaacatc
     8881 gacacgctcg agcgggtgta cctcgtcgac gtcgatcccg gcgagagcgg ctcgaccagc
     8941 gcgaaggtgc acgcgttctc cgagctgctc gcgtcgtcgg acggtccggc tcccgacccc
     9001 accgtcgagc tggacgaggg cgaccacgcc gtcatcttct acacgtcggg caccaccggc
     9061 cgtccgaagg gcgccatcag cacccaccgc tcgatgatcg ccaacctcca gaacacggtg
     9121 ttcctgatca ccgcgacgac ggcgatcatc aacgagaagt ctgctgccgc cggtggcgag
     9181 ccaccggcga cgcccacgca accggtcgcc ctgttcacct caccgctctt ccacgtgtcc
     9241 ggctgccact cgacgctggt cgtcggctcg ctgggcgggc tcaagctcgt gatgatcaag
     9301 ggcaagttca cccccgaagg cgcactgcag ctcatctccg acgagcgggt gaccacctgg
     9361 tcgaccgtcc cgaccatgat ctggcgcacg tgcgaacacc cagaccgcca cgagtacgac
     9421 acctcgaccg tgaccgccgt cgcgttcggt ggatccccgt cggccgacga gctccagcgc
     9481 cgcatccgcg agacgttccc caacgtgaag ggcacgtcga acgcctacgg cctgaccgag
     9541 acctcgtcgg tggcgaccgt gctccacggc gacgccgcgc tcaccaaggc cgactccgtg
     9601 ggcctgccgg tgccgacagt acgaatcgcg atctccgacg gcaggggcgg cctcgccgag
     9661 ccgggggcca ccggtgaagt cctcatcggc gggcccgtcc tcatggccgg ctactggaac
     9721 aagcccgagg cgaccgcgaa ggccatccag gacggatggc tctacaccgg cgacgtcggc
     9781 cacatcgacg aggacggcta cctgttcatc accgaccggg ccaaggacat gatcatccgc
     9841 ggcggcgaga acatctactg cgtcgagatc gagaactgcc tcgtcgaaca cgaagccatc
     9901 gccgacgctg cgatcgtcgg cgtgccccac gaggggctcg gcgaggaggt caaggcgttc
     9961 atcgagttgg cgccgggagc gtcgctgacc gccgacgagg tgcaaggctg ggtggcgaag
    10021 cacctcgccc cgttcaaggt gccgacctac gtggagttca ccgacggcaa gctgccccgc
    10081 aacgcgtcgg gcaaactgct caagaacgtg ctgcgcggcg agggcgacgt gagctttcag
    10141 gagacgatgt gatcgtgacg acgagagaga cgatgtgagc gacgcaccga aagctccccc
    10201 ggttcccgac gggacctacg acgtgttcat cgtcgacgcc gtgccccacc ccgaccgtcc
    10261 cgagagtgtc gtgaaggtcg aggtcacgat cgtgagcggc gagttcaagg gcgaggtgct
    10321 caacctcagc gccaacggca tcagcggctc cgacatcgac ctcatgggca tgcccgcggc
    10381 actcacggtc acggacggtg tgcccgtgtt gaccgtggag ccctgacggc agagacaccc
    10441 gaccgctgac gggcgtcccc cactagcctc cctcgaacgc ggccccgggc gggcggccgt
    10501 ggtcggaacg acgggaggcg tgacgatgga ctgcacgaag tgcggtgggc ggggcgagta
    10561 ctacggcatc ggctcgaccg gtcgcttccg cgacgagccg atgatgcgcg actgtgaccg
    10621 ttgtggtcgg accggcatcg agccgggaac gaacggcgcg acggcaacgg gcagcgcgcc
    10681 cgcaggtggt tgcctctcga tgctgttcgg ctggcttgcc ggctggctcg cccgcatcgt
    10741 cctggcgctc gtcggcttgg cgatcctcgg cgcgatcgtg ttcgggttct cgtggtggaa
    10801 gagccgcaac cacgggctcg tcgaggcacg accgtcgtcc gaagtgacca ccgaggggta
    10861 cgccatcggc acctgtctga acgacccgtt cggcgccgca ctccctgtcg actgcggtca
    10921 accccacgag gcggaggtgt accgcatcct cgaggcgaag gaccccgaga ccgaggccta
    10981 ccccgggaac ggacggctcg acgacttcgc cgcggcgacg tgtccggagc acttcgagga
    11041 ctacgtcggg gccactccgg ccgacgccga cctgtcgatc ggcagccgcg gccccaccga
    11101 gaccgaatgg cgaatcggct cccgtcgggt cctgtgcttc gccg
//
LOCUS       DAOSOA010000076        11050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5895_length_11050_cov_5.741701, whole genome shotgun sequence.
ACCESSION   DAOSOA010000076 DAOSOA010000000
VERSION     DAOSOA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5895_length_11050_cov_5.741701"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PKY13_06770"
     CDS             <1..786
                     /locus_tag="PKY13_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79673.1"
                     /translation="LRRGPWHERLAGAANEQLVIHSTAHILRNVARISLEQTRELTAM
                     STELAAAGRLGRLREQRLAQRSLVHDGIAQVLGAVVAGDLDANVLGAWIDEEIARIGV
                     AIDGTERKPASIGDELHGLAAEFGRRGLNVVVEQSAPVPHWTRTATPTVTEIVREALN
                     NVAKHTDRRSATVAVESVDRSPDIAADGAIVVRVTDGGDERSNDVDVDVGVAERVGSG
                     VGTRSMAEAAASIGASIDWIPRVGGGTEVRLVLDPSAPQQQAV"
     gene            846..1457
                     /locus_tag="PKY13_06775"
     CDS             846..1457
                     /locus_tag="PKY13_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT79674.1"
                     /translation="MLPTSDTAALAPRTGSRASSMPADERRAAIAAATLPLLLEVGAG
                     ITTRQIAEAAGIAEGTIFRVFATKEDVIWAAVELAFDPTPVRDGIAAIDRSLPLRERL
                     EAAVAQIQDRTSRIFQLMGVIATIAPDRQGPPLPRKPPGALDGLVDLFLPDADSLSCE
                     PHVAAQVLRSITFGGTHPMFITEDGVLSPSDIVELALGGILAR"
     gene            1506..3239
                     /locus_tag="PKY13_06780"
     CDS             1506..3239
                     /locus_tag="PKY13_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT79675.1"
                     /translation="MLWQLLRTYIRPYRKQLYLVVGLQLVATIAALYLPSLNADIIDQ
                     GVVRQDTGYIWSHGGIMLAVSLVQVLFAIAGVFFGARAAMGFGRDVRHALFHQVTGYS
                     AKEINELGAPSLITRITNDVTQVQMLVLMGCTMFVAAPITTVGGVIMALREDIGLSAI
                     LIFSIPALVTLVGLVVRKMIPTFRVMQVRIDEVNRVLREQITGMRVVRAFVREPAEVK
                     RFGSANDDLTETSLTAGRLMACMFPSIMIVLNVSTVAAVWIGANRINSGDMQVGQMIA
                     FLSYLAQILMSLMMATFMAVMIPRAAVCAERLVEVLETDSSVEEAPDALAELPESAPL
                     ELRNVGFHYPGADEPILTDISLTVAPGTTTAVIGSTGSGKTTLLNLIPRLFDVTAGEV
                     LIGGVDIRTVRLETLWARIGLVPQKPYLFSGTVASNLRYSKPDATDEELWAALEVAQA
                     TDFVKAMPLQLDEPIVQGGFNVSGGQRQRLAIARALVRKPSIFLFDDSFSALDLATDA
                     RLRAALLPHTRDAAVVVVAQRVSTIMGADQILVLEDGHCVGLGTHRELLASCPTYIEI
                     VESQMTQEEAA"
     gene            3239..5143
                     /locus_tag="PKY13_06785"
     CDS             3239..5143
                     /locus_tag="PKY13_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT79676.1"
                     /translation="MTDQTNDHTDDTPVVKTEVVREARQAAGGRMGGMAGMPTEKSKD
                     FAGSTRRLMSRLTPERPGVTAVLLFAVISVTLSVIGPKVLGHATDILFKGIMAKFRGT
                     GSIDFDDMRNVLLAALGIYVVSAAFQWLQAFLLAGVVQRTMLRLRAEVEDKLHRLPLS
                     YVDGQPRGDLLSRVTNDIDNIAQSLQQTLSQLLTSVLSLLGVLIMMFVISPLLALVAL
                     ITIPASLVLIKSITGRSKKKFIAQWAHTGALNAQVEEAFTGHNLVKVFGRQADVEARF
                     GRKNEELFDAGFQAQFISGVIQPAMMFLGNLNYVVIAVVGGLRVASGALSLGDVQAFI
                     QYSRQFTMPLTQLASMINVFQSGVASAERVFELLDAPEQELEEDTAGRDVEVRGRVEF
                     EHISFSYDPANPLIQDLSLVAEPGQTVAIVGPTGAGKTTLVNLIMRFYELDDGRITLD
                     GRDIAKMPRNQLRSEIGMVLQDTWLFGGTIRENILYGNLDATEEQMLAAARATYVDRF
                     VHSLPDGYDTRIDDEGGTVSAGEKQLLTIARAFLADPAILILDEATSSVDTRTEVLIQ
                     SAMAALRSQRTSFVIAHRLSTIRDADTILVMEAGRIVEQGNHEQLLAADGAYARLYNA
                     QFAAAAAEVA"
     gene            complement(5167..5898)
                     /locus_tag="PKY13_06790"
     CDS             complement(5167..5898)
                     /locus_tag="PKY13_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT79677.1"
                     /translation="MATPNTQGTQPYVLVVDDEEHISEMVAMGLGFNGFEVDRAASGR
                     GALDAIAKRRPDLVVLDVMLPDLDGFEVARRLRQAEGAGTRVPVIFLTARDTTSDKVE
                     GLRLGSDDYVTKPFSIEELIERVKAVLRRSSGTGPGEQKLSYADLELDEETRDVWRAG
                     HLIELTPTEYKLLHYLLSNSRRVLTRDQILEHVWDYTFAGNASVLETYISYLRHKVDE
                     YDPPLIHTVRGVGYSLRLPPGHESA"
     gene            6029..7531
                     /locus_tag="PKY13_06795"
     CDS             6029..7531
                     /locus_tag="PKY13_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT79678.1"
                     /translation="MAIALRPSIVTRRTRVRQTARDARLPRTRHRMTLRFRLVVALLA
                     LLTLGMAVFGVVVTSFYSHSQYQRLDERLRNQAPFASARLRQELGLAGGFGNRADPNG
                     GPSQLGPDAYVQLRDTDGTVLGSGTFSDTTNVPAIPDEIDLGTDRAVFLTVEAAKGSG
                     RWRVLASADPFGGLVSVVAVPMSDVSGPLNRLILIEVLSAAGLLAILGTGSWLILRRG
                     LRPLEQMATSASSITAHSMQGGNLAERLSPSDSQTEVGQLGLAINTMLGEIEVAFRER
                     DATEQRLRQFLADASHELRTPLTSIQGFAELFRMGAAEDREKLAIILRRIEEESDRMK
                     VLVEELLLLARLDETRPPERSEVDLAVLCADACSDASATAPGRRVTLLAPAPVTVLGD
                     RDHLRQAIANLVTNALRHTPEGRPIEVGAAREGDTAVITVRDHGDGLDAAALEHAFDR
                     FWRADSARVGSGTGLGLAIVAAIADEHDGTATVENAPGGGAVFSIRLPAM"
     gene            7670..8116
                     /locus_tag="PKY13_06800"
     CDS             7670..8116
                     /locus_tag="PKY13_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79679.1"
                     /translation="MALFWWVASAADRLSTFSVLALPIAGLAVVSFSILRTIDESDGV
                     VIRVILVHAALAVLAVVAWTTDAPRSTRVMAAEWCLRSAQDGDTFDCGGWTGRVTVTT
                     DGWYYDIDDAAADLVLSPDAPTPASFAPTYRHLYGDWYLRAPWGAP"
     gene            8113..8709
                     /locus_tag="PKY13_06805"
     CDS             8113..8709
                     /locus_tag="PKY13_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79680.1"
                     /translation="MTSPFSADEQTTAEMAAEQLVAIARRQRRRLTTAHWGARLATTA
                     VIVWAFGQPDAVGLGPLVFSLAAVGGTAVIGNRRLLEAGPGPVVRIWSATALLGILAI
                     AGWANDWPVAARVQLAGSCLLATDDGGVFNCAGWSGWRSGDRFQIRGQRSWIEHHDKG
                     GPPLEPNGSSFTRTEAAALWGGWYLVEDLTFVDAGYWF"
     gene            complement(8801..9433)
                     /locus_tag="PKY13_06810"
     CDS             complement(8801..9433)
                     /locus_tag="PKY13_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79681.1"
                     /translation="MGRTSLLPAVAILLTGLTLGTTGCGDDKADIKAVATTTEKATTT
                     TEPEDDPTETTLAEDTGDTGESPFEEVDLGDELNPIEGYTYASLPTAAQEALISSFAA
                     DPSVTELVAAVGTTTVSDGTDTALLIFLGLNRELDATETQQFVDGVTAGGTNLEQGEV
                     AGQTGWAYVSADGTQGFITIRQDTVVIGQSDSVDHLSAVIQGLFVANPDL"
     gene            9602..10981
                     /locus_tag="PKY13_06815"
     CDS             9602..10981
                     /locus_tag="PKY13_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT79682.1"
                     /translation="MTLMEATRDEEAMSAKADAIRELSAHLHAANARLAELLADFTAD
                     DTWNVSGDWRSLAHWLGINCGFARAEAEILARVAAAAEAMPTVLERAGRGEVSLHVAA
                     AAARVATPENEAKLAEIAASSTPAQACRVFGNYRTAKRRDNERLDNEHRETEDGDPTA
                     TEPEHRSFWRTWFDDNGTWRVNGAAPPEIGALLDQLYQAGHKQVELSLDETANTHIDT
                     ADTLRAMADLGLDTADHRRLRDEGNERFLVTVLIDLETLVDNTIRPDSICRLQSGPHI
                     TPNLVRRLAEEGTLQLMWHRTGIPLKLGHETRLATREQKRALRFRDGGCTVPGCTAEK
                     HLHAHHIDWWPLGPTDIDNMVLLCRFHHRLLHRGHMSIRTDGTQTFVFRDRYGQEIGR
                     RGPPPGGPPGRLEDLPCHRNLGINADTARPHGGYWPLTNYARSVWVEALLTAGEPDPR
                     VDTTYAVAR"
BASE COUNT         1743 a         4050 c         3562 g         1695 t
ORIGIN      
        1 ctgcgccggg ggccgtggca cgagcggctc gcgggcgccg ccaacgagca actggtgatc
       61 cactcgacgg cgcacatcct ccgcaacgtg gcgcgcatct cgctcgagca gacccgggag
      121 ctcacggcga tgtcgaccga gctcgcggca gccggacgtc tcggccggct gcgcgaacaa
      181 cgtctcgccc aacggtcact ggtgcacgac ggcatcgccc aggtcctcgg agcggtcgtc
      241 gccggcgacc tcgacgcgaa cgtgctcggc gcgtggatcg acgaggagat cgcccgcatc
      301 ggggtcgcca tcgatggcac cgagcgcaag ccggcgtcga tcggcgacga gttgcacggc
      361 ctcgccgcgg agttcggccg ccgtggcctg aacgtcgtcg tcgaacagtc cgcgcccgtg
      421 ccgcactgga cccggaccgc gaccccgacg gtcaccgaga tcgtccgcga ggcgctcaac
      481 aacgtcgcaa agcacacgga ccgtcgatcg gcaacggtcg ccgtcgagtc ggtcgaccgg
      541 tcccccgaca tcgctgctga cggcgcgatc gtcgtgcgcg tgaccgacgg cggcgacgag
      601 cgttcgaacg acgtcgacgt cgacgtcggc gtggcggagc gcgtcgggtc gggggtcggc
      661 acacggtcga tggccgaggc ggcggcaagc atcggcgcct cgatcgactg gatcccccgg
      721 gtgggcggcg gcaccgaggt ccggctcgtc ctcgacccga gtgcacccca gcagcaggcc
      781 gtgtgaccct tttctcagcg taaagcgttc gtgagtgagt gctcactcag gtacggtgct
      841 ccaccgtgct gccgacctcc gacactgccg cgctcgcccc gcgaaccggc tcccgcgcga
      901 gttcgatgcc ggccgacgag cgacgggccg ccatcgccgc ggcaacgctg ccgctcctgc
      961 tcgaagtcgg cgccgggatc acgacccgtc agatcgccga agccgccggc atcgccgagg
     1021 gcacgatctt ccgggtcttc gcgaccaagg aggacgtgat ctgggccgcc gtcgagcttg
     1081 cgttcgaccc cacaccggtc cgtgacggca tcgccgcgat cgaccggtcg ctccccctcc
     1141 gggagcgact ggaggccgcg gtcgctcaga tccaggaccg cacatcgcgg atcttccagc
     1201 tcatgggggt gatcgccacg atcgcgcccg accggcaggg tccgccgctc ccccgcaagc
     1261 cccccggtgc gctcgacggg ctcgtcgacc tcttcctccc cgacgccgac tccctcagct
     1321 gcgagcccca cgtcgcagcc caggtgctcc gctcgatcac gttcggcggc acccatccca
     1381 tgttcatcac cgaggacggg gtgctctcgc cgtccgacat cgtcgagctc gcgctcggcg
     1441 gcatcctcgc ccgctgacct tcagccgctg cccctcaccc gccgacctcg atcatcagga
     1501 accacatgct ctggcaactc ctccgcacct acatccggcc gtaccgcaag cagctctacc
     1561 tcgtcgtcgg gttgcaactg gtcgcgacga tcgccgcgct ctatctcccg agcctcaacg
     1621 ccgacatcat cgaccagggt gtcgtgcggc aggacacggg ctacatctgg tcccacggcg
     1681 gcatcatgct cgccgtcagc ctcgtccagg tgctgttcgc aatcgccggt gtgttcttcg
     1741 gcgcccgcgc cgcgatgggg ttcgggcgcg acgtccgcca cgccctgttc caccaggtca
     1801 ccgggtactc cgccaaggag atcaacgagc tgggcgcgcc gtcgctgatc actcgcatca
     1861 ccaacgacgt gacgcaggtg cagatgctgg tgctgatggg ctgcaccatg ttcgtggcgg
     1921 cgccgatcac gaccgtcggc ggcgtcatca tggcgctgcg cgaggacatc ggactgagcg
     1981 ccatcctcat cttcagcatc ccggcgctcg tcacgctggt cggcctcgtg gtccgcaaga
     2041 tgatcccgac gttccgcgtc atgcaggttc gcatcgacga ggtgaaccgg gtgttgcggg
     2101 agcagatcac cggaatgcgc gtcgtgcggg cgttcgtgcg tgagcccgcc gaggtcaagc
     2161 gattcggctc ggccaacgac gacctcaccg agacgtcgct caccgccggt cgactcatgg
     2221 cctgcatgtt cccgtcgatc atgatcgtcc tcaacgtgtc gacggtcgcc gccgtgtgga
     2281 tcggcgccaa ccgcatcaac agcggcgaca tgcaggtcgg ccagatgatc gccttcctca
     2341 gttacctggc ccagatcctg atgtcgctca tgatggcgac gttcatggcc gtcatgatcc
     2401 ctcgcgccgc ggtgtgcgcc gagcgcctcg tcgaggtcct cgagaccgac tcgtcggtcg
     2461 aggaagcgcc cgacgccctc gccgagctgc ccgagtccgc gccgctcgag ctgcgcaacg
     2521 tcggcttcca ctatcccggc gccgacgaac cgatcctgac cgacatctcg ctcaccgtcg
     2581 cgcccggcac gacgaccgcc gtcatcggct cgaccggttc gggcaagacc acgctgctca
     2641 acctcatccc ccggctcttc gacgtgacgg cgggcgaggt cctcatcggc ggcgtcgaca
     2701 tccgcacggt gcgactggag accctgtggg cccgcatcgg gttggtgccc cagaagccgt
     2761 acctgttcag cgggaccgtc gcctccaacc tccgctactc caagcccgac gcgaccgacg
     2821 aggaactgtg ggcggcgctc gaggtcgcgc aggccaccga cttcgtgaag gccatgccac
     2881 tgcaactcga cgaaccgatc gtgcagggtg ggttcaacgt ctcgggtggg cagcggcagc
     2941 gcctcgccat cgctcgggcg ctggtccgca aaccctcgat cttcctgttc gacgactcgt
     3001 tctcggcgct cgacctggcg accgacgccc ggctccgcgc cgcactactc ccccacaccc
     3061 gtgacgccgc ggtcgtcgtc gttgcgcaac gcgtgtccac gatcatgggc gccgaccaga
     3121 tcctggtgct cgaggacggc cactgcgtcg gcctcggaac ccaccgtgag ctgctggcga
     3181 gctgccccac ctacatcgag atcgtggaat cgcagatgac ccaggaagag gccgcctgat
     3241 gaccgaccag accaacgacc acaccgacga cacacccgtc gtgaagaccg aggtcgtgcg
     3301 cgaggcccgc caggcggcgg gcggccgcat gggcggtatg gcggggatgc cgaccgagaa
     3361 gtcgaaggac ttcgcgggct ccacccgccg cctgatgagc cgcctcaccc ctgagcgccc
     3421 cggcgtgacc gccgtgctcc tgttcgccgt gatcagcgtg acgttgtcgg tcatcggccc
     3481 caaggtgttg ggccacgcca ccgacatcct gttcaagggc atcatggcca agttccgcgg
     3541 caccggcagc atcgacttcg atgacatgcg caacgtcctc ctcgccgcac tcggcatcta
     3601 cgtcgtctcc gctgcgttcc agtggctgca ggcgttcctg ctggccgggg tcgtgcaacg
     3661 aacgatgctg cgtctgcgcg ccgaggtcga ggacaagctg caccgcctcc ccctcagcta
     3721 cgtcgacggc cagcctcgcg gcgacctcct gagccgcgtc accaacgaca tcgacaacat
     3781 cgcgcagagc ctccagcaga ccctcagcca actgctcacg tcggtgctgt cactgctcgg
     3841 tgtgctgatc atgatgttcg tgatctcgcc gctgctggcg ctggtcgcgc tgatcaccat
     3901 ccccgcctcc ctcgtcctca tcaagtcgat caccggtcgg tcgaagaaga agttcatcgc
     3961 ccagtgggcc cacaccgggg cgctcaacgc gcaggtcgag gaggcgttca ccggccacaa
     4021 cctcgtgaag gtgttcggtc ggcaggccga cgtcgaggcc cggttcggcc gcaagaacga
     4081 ggagctgttc gacgccgggt tccaggcgca gttcatctcg ggtgtgatcc agccggcgat
     4141 gatgttcctc ggcaacctca actacgtcgt gatcgcggtc gtcggtggcc tccgggtcgc
     4201 gtcgggcgcg ctctcgctcg gcgacgtgca ggcgttcatc cagtactccc gccagttcac
     4261 gatgccgctg acccagctcg cctcgatgat caacgtgttc cagtccgggg tggcgtcggc
     4321 cgagcgggtg ttcgagctcc tcgacgcccc cgagcaggag ctcgaggagg ataccgccgg
     4381 ccgcgacgtc gaggtgcggg gtcgtgtcga gttcgagcac atctcgttct cctatgaccc
     4441 ggcgaatccg ctgatccagg atctgtcgct cgtcgccgag cccggccaga ccgtcgccat
     4501 cgtcggcccg accggcgccg gcaagacgac gctcgtcaac ctcatcatgc ggttctacga
     4561 gctcgacgac ggccggatca ccctcgacgg ccgcgacatc gccaagatgc cccgcaacca
     4621 gctccgctcc gagatcggca tggtgctgca ggacacgtgg ctgttcggcg gcacgatccg
     4681 cgagaacatc ctctacggca acctcgacgc caccgaggag cagatgctcg cggcggcccg
     4741 ggcgacctat gtcgaccggt tcgtgcactc gctgcccgac gggtacgaca cccgcatcga
     4801 cgacgagggc ggcacggtgt cggccgggga gaagcagctg ctcacgatcg cccgggcgtt
     4861 cctcgccgac cccgcgatcc tgatcctcga cgaggcgacc agctcggtcg acacccgcac
     4921 cgaggtgctc atccagagcg ccatggccgc gctgcgctcc cagcgcacga gcttcgtgat
     4981 cgcccaccgc ctgtccacca tccgcgacgc cgacacgatc ctggtgatgg aagccggacg
     5041 catcgtggag cagggcaacc acgagcagct gctggccgcc gacggcgcct acgcccgtct
     5101 ctacaacgcc cagttcgccg cagccgccgc cgaggtggcg tagacccgaa gctcaccggt
     5161 ttggcatcag gcgctctcgt gacccggggg gagcctgagg ctgtagccga cgccgcggac
     5221 cgtgtggatg aggggcgggt cgtactcgtc gaccttgtgg cgcaggtagc tgatgtaggt
     5281 ctcgaggacg ctggcgttgc ccgcgaacgt gtagtcccac acgtgctcga ggatctggtc
     5341 gcgggtgagc acccggcggg aattcgacag cagatagtgc aacagcttgt actcggtcgg
     5401 cgtcagttcg atcaggtgac cggcccgcca cacgtcacgg gtctcctcgt cgagctcgag
     5461 gtcggcgtag gagagcttct gctcccccgg acccgtgcca ctcgagcggc ggagcaccgc
     5521 tttcacccgc tcgatcagct cctcgatgct gaacggcttg gtgacgtagt cgtcgctgcc
     5581 gagccggagg ccctcgacct tgtcgctcgt ggtgtcacgc gccgtgagga agatgacggg
     5641 cacccgcgtg cccgcgccct cggcctggcg aaggcggcga gcaacctcga acccgtcgag
     5701 gtcgggcagc atcacgtcga gcaccacgag atcgggacgc cgcttggcga tcgcgtcgag
     5761 cgcgccgcgc cccgatgccg cccgatccac ctcgaagccg ttgaacccga gacccatggc
     5821 gaccatctcg ctgatgtgct cctcgtcgtc gaccacgagg acgtagggct gggttccttg
     5881 ggtgttgggg gttgccatac gcccatggtg acgcagggac gaccacatgt ggcgcgcatc
     5941 tcgcggactt cttacggtac ggacgattcg gagcgaactc ccagaatcca ctccgccgac
     6001 gcggagcttc gaggtcgaca gtgaacgagt ggccatcgca ctccgtcctt cgatcgtcac
     6061 ccgccggacg cgggtgcgtc agactgctcg ggatgcccga ctgccccgca cccgccatcg
     6121 gatgacgctc cggttccgcc tcgtcgtcgc gctcctggcg ctgttgacgc tcggcatggc
     6181 ggtgttcggc gtggtcgtca cgtcgttcta ctcccactcg cagtaccagc ggctcgacga
     6241 acgactccgc aaccaagcgc cgttcgcgtc ggcgcggctg cgccaggagc tcggcctcgc
     6301 cggcgggttc ggcaaccgcg ccgatcccaa cggcggaccg tcacagctcg gccccgacgc
     6361 ctacgtgcag ctccgcgaca ccgacggcac ggtcctgggg agcggcacct tctccgacac
     6421 gacgaacgtg ccggcgatcc ccgacgagat cgacctcggc accgatcgag ccgtgttctt
     6481 gaccgtggag gcagcgaagg gatcggggcg ctggcgggtc ctcgcctccg ccgacccctt
     6541 cggcgggctc gtcagcgtgg tcgccgtgcc gatgagcgac gtgagcggcc cgctcaaccg
     6601 cctgatcctc atcgaggtcc tgtcggcggc cggcctgctc gcgatcctcg gcaccgggtc
     6661 gtggctcatc ttgcgccgcg gcctgcgacc gctcgagcag atggcgacgt cggccagctc
     6721 gatcaccgcc cactcgatgc agggcggcaa ccttgccgaa cgcctctcac cgtcggacag
     6781 tcagaccgag gtcggccagc tcgggctcgc catcaacacg atgctcggcg agatcgaggt
     6841 ggcgttccgc gagcgtgacg ccaccgagca acgcctgcgc cagttcctgg ccgacgcctc
     6901 ccacgagctg cgcacgccgt tgacctccat ccaggggttc gccgagctgt tccgcatggg
     6961 tgcggccgag gatcgggaga agctcgccat catcctgcgg cgcatcgagg aggagtccga
     7021 ccgcatgaag gtgctggtcg aggagctgct cctgttggcc cgcctcgacg agacccgccc
     7081 tcccgagcgg agcgaggtcg atctcgcggt gctgtgcgcc gacgcctgca gcgacgcctc
     7141 ggcgaccgcg ccgggccgcc gggtgaccct cctcgccccc gcgccggtga cagtgctcgg
     7201 cgaccgcgac cacctgcgcc aggccatcgc caacctcgtg accaacgccc tgcggcacac
     7261 ccccgagggc cgccccatcg aggtgggcgc ggcccgggag ggtgacactg ccgtcatcac
     7321 cgtgcgggac cacggcgacg ggctggacgc cgccgcgctg gagcacgcgt tcgaccgctt
     7381 ctggcgggcg gacagcgccc gcgtcggcag cggtacgggc ctcgggctcg cgatcgtcgc
     7441 cgccatcgcc gacgagcacg acggcaccgc caccgtcgag aacgcgccgg gcggcggcgc
     7501 tgtgttcagc atccgcctcc ccgcgatgtg aggcctgtgc aaacggtgtg aacagccggt
     7561 gaagcccgtg ccgcccgatc gccgagggcc ctaccgtcga cctcgtggag gtgagcgaag
     7621 gatcggaccc gtcggtgcgg tggggccatc gcatcgtggt cgtcgctgga tggcgctgtt
     7681 ctggtgggtg gcgagcgcgg ctgaccggct ctcgacgttc tcggtcctgg cgctgccgat
     7741 cgccggcctc gccgtcgtgt cgttctcgat cctgcggacg atcgacgagt ccgacggcgt
     7801 cgtgatccgc gtgatcctcg tccatgcggc cctcgccgtc ctggcggtcg tcgcgtggac
     7861 gaccgacgca ccccgctcca ctcgggtgat ggccgccgaa tggtgcctgc ggtcggcaca
     7921 ggacggagac acgttcgact gcggtggatg gacgggcaga gtgacggtca cgaccgatgg
     7981 ctggtactac gacatcgacg acgccgccgc cgacttggtg ctcagccccg acgcgccgac
     8041 accggcaagc ttcgcaccga cgtaccgcca cctctacggg gactggtacc tccgggcacc
     8101 gtggggcgcg ccgtgaccag cccgttcagt gccgacgagc agaccacggc cgagatggcg
     8161 gccgagcagc tcgtggccat cgcacggcga caacggcgtc gactcacgac cgcgcactgg
     8221 ggagcacgct tggcgacgac agcggtgatc gtctgggcct tcggccaacc tgacgccgtc
     8281 ggcctcggtc cgctcgtgtt ctcgctcgct gccgtgggag gaactgcggt cattggcaac
     8341 cgtcgtctcc tcgaggccgg gcctggcccg gtcgtgcgga tctggagcgc gaccgcactg
     8401 ctcggcatcc tcgccatcgc cgggtgggcg aacgactggc ccgtcgccgc ccgggtgcaa
     8461 ctggcggggt cgtgcctcct cgccaccgac gacggtgggg tgttcaactg cgcaggatgg
     8521 agcgggtggc ggtcgggcga ccgcttccag atccggggcc aacgctcctg gatcgaacac
     8581 cacgacaagg gaggtccacc gctcgaaccc aacggatcgt cgttcacccg caccgaggcc
     8641 gcggcgctat ggggcggctg gtacctggtc gaggacctga cgttcgtgga tgccggctac
     8701 tggttctgac cgagagcgga cgcgatccag gctcagccgg gcacgagtgc ccggctgagc
     8761 ctggatcggg gatctcgctg gagaggtgct gaggtcccga tcagagatcg gggttggcga
     8821 cgaagaggcc ctggatcacg gcggacaggt ggtcgacaga gtcggactga ccgatgacga
     8881 cggtgtcctg gcggatcgtg atgaacccct gcgtgccgtc ggcgctgacg tacgcccatc
     8941 ccgtctggcc ggcgacctca ccctgctcca ggttggtgcc gccagcggtg accccgtcga
     9001 cgaactgctg ggtctcggtc gcgtcgagct cgcggttgag gcccaggaag atgaggagcg
     9061 cggtatcggt gccgtcggaa acggtcgtgg ttccgactgc ggcgaccagc tcggtcaccg
     9121 agggatcggc ggcgaagctg gagatcagcg cctcttgggc ggctgtcggc aatgaggcgt
     9181 acgtgtaccc ctcgatcggg ttgagttcgt cgccgaggtc gacctcctcg aacggactct
     9241 caccggtgtc gcccgtgtcc tcggcgaggg tcgtctcggt cggatcgtcc tcaggctcgg
     9301 tggtcgtggt ggccttctcg gtcgtcgtcg cgacggcctt gatgtcggcc ttgtcgtcgc
     9361 cgcacccagt ggtgccgagc gtcagcccgg tcagcaggat ggcgacggcg gggaggaggg
     9421 aagttctgcc catgatgtgg atgtggctcc tgttcggccc cacccggtgt ggagcgaccc
     9481 ctccatcata gggcgaaccc ggcccgaatc gtcgcgtgta cgagagcgcc gccccaggga
     9541 gactgggcaa atcatcccgg tgagcgaaca ccagttcgat caccacgcta tacttccagc
     9601 catgaccttg atggaggcga cacgtgacga ggaggcgatg tcggcgaagg ccgacgccat
     9661 ccgcgagctg tccgcgcacc tccacgctgc gaacgcacga ctggccgaac tgctcgccga
     9721 cttcaccgcc gacgacacct ggaacgtctc cggcgactgg cggtcactcg cccactggtt
     9781 gggcatcaac tgcggattcg cccgcgccga ggccgagata ctggcccgtg tcgccgccgc
     9841 cgccgaggca atgccgaccg tcctggaacg cgccggccgc ggcgaagtgt cgttgcacgt
     9901 ggccgcggcc gccgcacgcg tcgccacccc ggagaacgaa gccaaactcg ccgagatcgc
     9961 ggcgtcgtcg acaccggccc aggcctgccg agtcttcggc aactaccgca ccgccaagcg
    10021 ccgcgacaac gaacggctcg acaacgaaca ccgcgagacc gaagacggtg accccacagc
    10081 aaccgaaccc gagcatcggt cgttctggcg gacctggttc gacgacaacg gcacctggcg
    10141 ggtcaacggc gccgccccac ccgaaatcgg cgcactcctc gaccagctct accaagccgg
    10201 ccacaaacag gtcgaactgt ccctcgacga aacggcaaac acccacatcg acaccgcaga
    10261 caccctccgc gccatggccg acctcggcct cgacaccgcc gaccaccgac gcctccgcga
    10321 cgaaggcaac gaacgcttcc tggtcaccgt cctcatcgac ctcgaaaccc tcgtcgacaa
    10381 caccatccga cccgactcca tctgccgcct ccaatccggc ccccacatca cccccaacct
    10441 cgtacgacgc ctcgccgaag aaggcaccct ccaactcatg tggcaccgca ccggcatccc
    10501 cctcaaactc ggccacgaaa cccgactcgc cacccgcgaa caaaaacgtg ccctccgctt
    10561 ccgcgacggc ggctgcaccg tccccggctg caccgccgaa aaacacctcc acgcccacca
    10621 catcgactgg tggcccctcg gaccaaccga catcgacaac atggtgctcc tctgccgatt
    10681 ccaccaccgc ctcctgcacc gaggccacat gtcgatccgc accgatggca cccaaacctt
    10741 cgtgttccgc gaccggtacg gccaagaaat cggccgacga ggaccacccc caggcgggcc
    10801 acccggccgc ctcgaagacc tcccctgcca ccgcaacctc ggcatcaacg ccgacacggc
    10861 gcgaccccac ggcggctact ggcccctcac caactacgcc cgcagcgtct gggtcgaagc
    10921 actcctcacc gccggcgaac ccgacccccg cgtcgacacc acctacgcgg tcgcgaggta
    10981 gctcagaccg gcgtgccgcc gaccgccgtc tgttcggcga cctcgccggc gagcagctgc
    11041 cgccaccgat
//
LOCUS       DAOSOA010000077        11038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5906_length_11038_cov_6.682327, whole genome shotgun sequence.
ACCESSION   DAOSOA010000077 DAOSOA010000000
VERSION     DAOSOA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_11038_cov_6.682327"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKY13_06820"
     CDS             <1..332
                     /locus_tag="PKY13_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79683.1"
                     /translation="IHGTADTVIRYEGGRNDDADPNAPAYPSAAQSVLEWAKFDGCDD
                     TAAPGDPSSRDLEKAMPAATVTSYSGCRAGTAVELWTQPDGEHVPELSDSFTAQVIDF
                     LLAHPKG"
     gene            complement(357..1796)
                     /locus_tag="PKY13_06825"
     CDS             complement(357..1796)
                     /locus_tag="PKY13_06825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HOT79684.1"
                     /translation="MRRTYVLVASLLAFGTVACTPAAPGNATVPIDEARTARAQAAPS
                     QPAPDGTSWSVYLTVDLGNHIAKVCSGSLISQRSVLTAAHCVERDDGSVAPASAAWAL
                     VGAGDAAGRPAVERNVVAIDVHPQRSYRYIGSDVVASSYDAALITLAAPVNSSPLPLA
                     IDGASVSPGPIVTFGYGYVPAQTPMNADGTRSSLVTVSPSDKLGIAPCTDRDVSADHL
                     CMASVDPAVWGTAPGDSGGPWVRTVRGGQVQVAVTSYGAVDRPGDSVGTNVLDILPWV
                     RATAGLLQVPAGTVVRDPATGSAWRVQRDGYRRLVPTDAALACLTEAGVPVAEHDLVT
                     IEQIPEAVGEVENCALAQRSVAVSPVTTITSPAATPTSLAVVTTTPSPVVVNAPAIAA
                     PIAAEAAAPPAASPAPTAVAGATQPTRPAGTVAVWTYEGPVVSSTDTWLAITGGDQRL
                     LLGGALLLLGTGAAAVTVQLRLRRADARR"
     gene            complement(1902..2408)
                     /locus_tag="PKY13_06830"
     CDS             complement(1902..2408)
                     /locus_tag="PKY13_06830"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOT79685.1"
                     /translation="MAVLLLNASFEPLHVIPLRRAMGLVVAGKVDVIANGDGEIHSAT
                     QTFPIPAVLRLRYMVRVPFRQNIALSRRAVLIRDQRRCQFSHCDRTASTIDHVVPRSL
                     GGEHTWTNVVAACGPCNTRKGSHLLKELGWTLKQRPVAPRGTVVLLSSAGVHTVPDVW
                     ADWLPALA"
     gene            complement(2507..2938)
                     /locus_tag="PKY13_06835"
     CDS             complement(2507..2938)
                     /locus_tag="PKY13_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79686.1"
                     /translation="MIAAVLAVEDSRISSMFVSFFIALLIVSPFGYRRIKATLAERRE
                     LLGRVDPDAGPTESGATTGADPDDLATVFDAIAVAASELSGEPDGHRVVDVPTSPTVE
                     GRPADPALVDALLDDAVRRSGLVAERSTRPDGGRRLRLVRA"
     gene            complement(3224..3832)
                     /locus_tag="PKY13_06840"
     CDS             complement(3224..3832)
                     /locus_tag="PKY13_06840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT79687.1"
                     /translation="MTRLLLVRHGQSTWNAMGRWQGQADPPLTPLGRRQAAEASAAIG
                     AVDAVFSSTLERARVTAEIISEQIGVGPVVSLPGLVERTAGDWEGLTRVEIEEQFPGY
                     LDAGDRPPGWEDDEVLEERAMSALGAIVVGLGEERGDVDLDVVVVTHGGLIYQVERRF
                     GAPFERMSNLGARWIELRRGEWHLGDRVDLLHGVDVTIPDQI"
     gene            3875..4117
                     /locus_tag="PKY13_06845"
     CDS             3875..4117
                     /locus_tag="PKY13_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOT79688.1"
                     /translation="MPTLRLFAQARVAAGTGRDDLPGDTVGEVLDGAIDRYGSGFAAV
                     LDISKIWVNGDEADRSTAVADGDEVAVLPPVSGGCG"
     gene            4122..4922
                     /locus_tag="PKY13_06850"
     CDS             4122..4922
                     /locus_tag="PKY13_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79689.1"
                     /translation="MQRVLDLVRTYAVRQPVGGPRLRLGVIWFLALLVALVVGSFAIT
                     VLFGLIATIGGLQVAGLWKHRKVAVNQLVAGGGAGLMALGAWSSVRAAAVAIVAMAFA
                     ALVLGTDNELGRSSFTKARLSSTLPVASATLRSGLFLGLAAAATVQVHRVDPMSFLYL
                     MSVACVYDCGDYLIGSGSRNRLVGPVAGLVGVLVVVIAMTAINPPPLLDDGDVRKLGV
                     AMALACPLGQLLGSWTLPSSRAKAPALRRLDSWLLTAPACLVGLWVLT"
     gene            4992..>5206
                     /locus_tag="PKY13_06855"
     CDS             4992..>5206
                     /locus_tag="PKY13_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79690.1"
                     /translation="MEGEPMPNPSGRQETPIVITELDELLLANDLGSLQSRPIEELRA
                     LRDQLGEIEAGLSFVRRMAQGRLDIVLA"
     assembly_gap    5207..5306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5533..5901
                     /locus_tag="PKY13_06860"
     CDS             5533..5901
                     /locus_tag="PKY13_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT79691.1"
                     /translation="MKDEIDKRWHDLGEFIREQRNVGQMSLRKLSELAGVSNPYLSQI
                     ERGLRKPSAEILQQIARALEISSETLYVRAGILDEPDGVPDLIGEIRRDPFIGEDQKK
                     TLVRIYESFRRENDAGVAEA"
     gene            5971..7179
                     /locus_tag="PKY13_06865"
     CDS             5971..7179
                     /locus_tag="PKY13_06865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT79692.1"
                     /translation="MLSLHTSPLAQPGEGDSGGMNVYVRELAASLAQAGVPTSVYVRH
                     WREGLAPRVSVEPLFEVVHVPVGPYDLAKEQLPAVADEFADWVGADIEARGDVTVLHA
                     NYWLSAVAAHRLKHELELPLVTTFHTLAQVKTATGDIEPGQRAEAERVVIGCSDAVCA
                     SNGVEADQLIDFYGADRGRIEIVPPGVDHAFFAPGDRRGARWALGLGAEPVLLFVGRI
                     QPLKGLDVAVAALGAMRSQTATLVAVGGPSGAEGDAELARIQAMIGDLGLDARVRLVA
                     PQPHHMLSTYYRAADVCVVPSRSESFGLVAAEAMACGTPVVATAVGGLTTLVDDGVTG
                     LLMPNRDPNAWAAALDGLIEHPLRLAAMSAAAAERGRGYTWSTTAARLRRVYSDLSRS
                     RVPATCGVVG"
     gene            7176..7649
                     /locus_tag="PKY13_06870"
     CDS             7176..7649
                     /locus_tag="PKY13_06870"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOT79693.1"
                     /translation="MSIDEEMPASTPEELARLADLIDAWVQRQIDEPWVAHVERGEGS
                     EPRWFIRVNGEEKSVFSIWFMLRQRSLFVETYVLPAPEENAAAVYEYLLRRNLRIFGM
                     SFAIGEEDAIYLTGEVHNRDVTDAELDRLLGTAYLQTEQCFRPAMRLAFASKFSG"
     gene            7986..8777
                     /gene="proC"
                     /locus_tag="PKY13_06875"
     CDS             7986..8777
                     /gene="proC"
                     /locus_tag="PKY13_06875"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOT79694.1"
                     /translation="MTRLQIVGGGRMGEALIGGILSSGWAEATDLRIVEASSARREQL
                     ASLFPGAEITTEPGAADGTIVATKPADIAAVTAAVSASGGGRILSIAAGVTLATLEAA
                     AGSGVAVVRAMPNTPALIGAGASAIAGGAAAEDADLDWAESLLGAVGAVVRVTEKQLD
                     AVTGLSGSGPAYVFLIAEAMVDAGVAAGLPRDLSTTLAYQTLVGSARLLAEGDASPAD
                     LRAAVTSPGGTTAAGVQVLERNAVRAAVIDAVLAARDRATELSAS"
     gene            8774..9712
                     /locus_tag="PKY13_06880"
     CDS             8774..9712
                     /locus_tag="PKY13_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOT79695.1"
                     /translation="MSEPAEGVDNADPRTYDTVTLLSDLGTADGFVGVIHSVIRQMAP
                     GAGVVDLTHGIAPFDVRAGSLVLARSVQFLCPGVIIGMVDPSVGTEQRAIALEVAGGA
                     AVLLGPDNGLLAPAVGMIGGAERAVVLDNTDLHLPTASTTFPGRDIYAPVAAHLCNGV
                     AFADLGSEVDPGSLMPGLVPLTSLEQDADGVDVLGAEVLWVDHFGNVQLNVDPDELDG
                     WGDRIQLRIGTNVRTATRITAFGQLTTGEIGLLVDADGLVAVVTDRHPAAEELGLATG
                     DAVALSPIGDDQAIGVRTAVTIGRRDPASTPPGGAS"
     gene            complement(9819..10706)
                     /locus_tag="PKY13_06885"
     CDS             complement(9819..10706)
                     /locus_tag="PKY13_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT79696.1"
                     /translation="MTAVLDERSLDLDGREPTDAPTHRPATGLDWTMIRALLAKDISA
                     ARRSKAVVIPMLVVPFVLLVGLPTGIGLFARTGTAPDLSGLMSHLPTSMAQDLQSLPQ
                     RQQLIELVLGYLIAPLFLIVPMMISSALAADTFAGEKERRTLESLLHLPVEERDLYVS
                     KVLFAFLPTVAASWIGFTLFAVSANVVAWPVMGRLFVPTWRWIGLIGFMAPAVAALGL
                     GVMVRVSARARTTQEANQLGGAVIMPLILASVGQTSGLLMMPPYWIFVAGGVVWVLAL
                     LLIRGGLVRFTRDRVASRL"
     gene            complement(10703..>11038)
                     /locus_tag="PKY13_06890"
     CDS             complement(10703..>11038)
                     /locus_tag="PKY13_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79697.1"
                     /translation="AAERWPGLEVHLDLGASPSLRVGQALLDHHAVMAMMPVADGAVI
                     NVRGREDIPAVVALVVGLGATVYGTQARPKGLEDVYFAVQDDLQRSLATTPPARPDAA
                     SSDATERAA"
BASE COUNT         1574 a         3823 c         3891 g         1650 t
ORIGIN      
        1 agatccacgg cacggctgac accgtgatcc ggtacgaggg cgggcgcaac gacgacgccg
       61 acccgaacgc gccggcctac ccgagcgctg cgcagtcggt cctcgagtgg gcgaagttcg
      121 acggctgcga cgacacggcg gcgcccggcg acccgtcgtc gcgggatctc gagaaggcga
      181 tgccggcagc gacggtcacc tcctacagcg ggtgtcgggc cggcaccgcc gtcgagctgt
      241 ggacccagcc cgacggtgag cacgttcccg agttgagcga ctcgttcacg gcgcaggtga
      301 tcgacttcct cctcgcgcac ccgaagggct gagagcgcgc cggcggcgtc tcgtcctcag
      361 cgccgggcgt ctgcccgtcg aagccggagt tgcaccgtga ccgcggcggc gccggttccc
      421 aggagcagga gcgctccgcc gaggagcagg cgttggtcgc cgccggtgat ggcgagccag
      481 gtgtcggtcg acgacacgac ggggccctcg taggtccaca ccgcaaccgt ccccgccggc
      541 cgcgtcggtt gcgtcgctcc ggcgactgcg gtcggggcgg ggctcgccgc gggaggcgcc
      601 gccgcctcgg cggcgatcgg tgccgcgatc gccggggcgt tcacgaccac cggtgagggt
      661 gtggtggtca ccacggcgag gctcgtgggt gtggccgccg gcgaggtgat cgtcgtgacg
      721 ggcgagacgg cgacggatcg ttgggcgagg gcgcagttct ccacctcgcc gacggcctcg
      781 gggatctgtt cgatcgtgac gagatcgtgc tcggcgacgg gtacgcccgc ctcggtcagg
      841 catgcgagcg cagcgtcggt cggcaccagt cggcggtagc cgtcgcgttg cacgcgccac
      901 gccgaacccg tggcggggtc gcgcaccact gtcccggcgg ggacctgcaa cagaccggcc
      961 gtcgcccgca cccacggcag gatgtccaac acgttggtgc ccacgctgtc gccgggacgg
     1021 tcgaccgccc cgtacgaggt caccgcgacc tgcacctggc ctcctcggac ggtgcggacc
     1081 cacgggccgc cgctgtctcc cggtgccgtt ccccatacgg ctgggtcgac actggccatg
     1141 cagaggtggt cggccgacac gtcccggtcg gtgcacggag cgatgccaag cttgtcggat
     1201 ggcgacaccg tcaccaggct cgatcgggtg ccgtcggcgt tcattggcgt ctgcgccggc
     1261 acgtagccgt agccgaacgt cacgatcggc ccgggcgaga ccgaagcacc gtcgatcgcg
     1321 agcgggagcg gcgagctgtt gaccggagcg gcgagcgtga tcagcgcggc gtcgtagctc
     1381 gacgcgacga cgtcgctccc gatgtagcga tagctgcgct gcgggtgcac gtcgatcgca
     1441 accacgttgc gttcgacggc cggtcgtccg gcagcgtcgc ccgcgccgac gagtgcccag
     1501 gccgccgatg ccggcgcgac cgagccgtcg tcacgttcca cgcagtgcgc cgccgtgagg
     1561 actgaccgtt gggagatcag actgccggag cacacctttg cgatgtggtt gccgaggtcg
     1621 accgtgaggt acaccgacca cgatgtcccg tcgggtgccg gctggctcgg cgccgcctgc
     1681 gctcgcgccg ttcgcgcttc gtcgatcgga acggtggcgt tgcccggcgc cgcgggggtg
     1741 caggcaaccg tgccgaacgc gaggagcgac gcgacgagaa cataggtgcg ccgcatgatc
     1801 cccttccgcc cggtcgccgg ctgggacgct accgagccgc tgcggccggg ggcaacgagt
     1861 ccttcgaagt gttcaccggc ccgttgcccg ccggccgcca cctacgcgag cgctggtagc
     1921 cagtcggccc acacgtcggg caccgtgtga acgcctgccg acgagagcag cacgacggtg
     1981 ccccgcggag cgaccggccg ctgcttcagg gtccagccga gctccttcag caggtggctg
     2041 cccttcctcg tgttgcaggg cccgcaggcg gcgacgacgt tggtccaggt gtgctcgccg
     2101 ccgagggaac gcgggacgac gtggtcgatc gtcgaggcgg tgcggtcgca gtggctgaac
     2161 tggcaccggc gctggtcgcg gatcaggacc gcccgccgtg acagcgcgat gttctgacgg
     2221 aacggcaccc gaaccatgta gcgcaggcgg aggaccgcag ggatggggaa cgtctgcgtc
     2281 gccgaatgga tctcgccgtc accgttggcg atgacgtcga ccttgcccgc caccacgaga
     2341 cccatggcgc gtcgcagggg aatgacgtgc agtggttcga acgatgcgtt gagcaacaac
     2401 accgccacgg cgcgctcctt cctgtgacgt cagagcatcg tcctccgagg aggggtcctg
     2461 ggcaacagaa ttacgcgtga accgccggcg cgcacggcat cccgcctcac gctcggacga
     2521 gtcggagccg gcggccaccg tcgggacgcg tcgaccgttc ggcgacgagc ccgctgcggc
     2581 gcacggcgtc gtcgagcaac gcgtcgacca acgccgggtc cgccggacgg ccttcgaccg
     2641 tcggggacgt cggcacgtcg acgacccggt ggccgtcggg ttcgccggac agttcggagg
     2701 cagcgacggc gatcgcgtcg aacaccgtgg cgagatcgtc ggggtcggcg ccggtcgttg
     2761 cacccgattc cgtcgggccg gcgtcggggt cgacacggcc caggagctct cggcgctcgg
     2821 cgagcgtcgc cttgatccgc cggtagccga acggcgacac gatcagcagg gcgatgaaga
     2881 aggacacgaa catcgaactg atgcggctgt cctcgactgc cagtaccgct gcgatcacgg
     2941 cgaagatcct acggtgacgt cgatctgagc ccgaaccgac gcacgcccta ggctgccggc
     3001 cgtgcagacc gatcccacga acaccgccgc cgacacgacc gcgctcgtca tcgacgaggt
     3061 ggagctcgag cgtctcgaac gtgatctggc cgacgctgcg gacacgctcg agtccatcga
     3121 gcggatcaca gcgtcgggcg cgagcggcgc cgagacggca tcggccatcc gccagttgct
     3181 cgacgacggt cggttcgacc tcacggcagg ctccgacgac gggtcagatc tggtcgggga
     3241 tggtgacgtc gacaccgtgc aggagatcga cgcggtcgcc gaggtgccac tcgccgcgac
     3301 ggagctcgat ccagcgggca ccgaggttgg acatgcgctc gaagggggca ccgaatcgcc
     3361 gctccacctg gtagatgagg ccgccgtggg tcacgacgac cacgtcgagg tcgacatcgc
     3421 cgcgttcctc cccgaggccg acgacgatcg ccccgagggc tgacatcgcc cgctcctcga
     3481 gcacctcgtc gtcctcccag cccggcggcc ggtcgccggc gtcgaggtag ccggggaact
     3541 gctcttcgat ctcgacgcgg gtgaggccct cccagtcgcc ggccgtccgt tcgaccaggc
     3601 cgggaagcga gaccaccgga cccaccccga tctgttcgga gatgatctcg gcggtcacgc
     3661 gagcccgttc gagcgtcgag ctgaacaccg cgtcgacggc gccgatcgcg gcgctcgcct
     3721 cagccgcctg tcggcgcccg agcggtgtca gcggcgggtc ggcctggccc tgccaacggc
     3781 ccatcgcgtt ccaggtcgat tgtccgtgcc ggacgaggag gaggcgggtc accggggcca
     3841 cggtagggcg cggaccgacg actacgctca tcccgtgccc acgctgaggt tgttcgccca
     3901 ggcccgcgtc gccgccggca ccggccgcga cgacctcccg ggcgacaccg tcggcgaagt
     3961 tctcgacggg gcaatcgacc ggtacgggtc cggcttcgcc gcggtgctcg acatctccaa
     4021 gatctgggtc aacggcgacg aggccgaccg ctcgacggcg gtggccgacg gcgacgaggt
     4081 cgctgtcctt ccgccggtct cgggtgggtg cggctgaggt ggtgcagcgc gtcctcgacc
     4141 tggtccgcac ctacgctgtt cgccaaccgg tgggcggccc acggctgcgc ctcggtgtca
     4201 tctggttcct cgcgctcctg gtcgccctcg tcgtcgggtc gttcgcgatc accgtgctgt
     4261 tcggtctgat cgccacgatc ggcggattgc aggtcgccgg cctgtggaag caccgcaagg
     4321 tggcggtcaa ccagctcgtc gccggtggcg gtgcggggct catggcgttg ggagcgtgga
     4381 gcagcgtgcg cgccgccgcc gtcgcgatcg tggcgatggc gttcgccgcg ctcgtcctcg
     4441 gcaccgacaa cgagctcggc cgctcgtcct tcacgaaggc gcggttgtcc tcgacgctgc
     4501 cggtggcatc ggcgaccctc cgatcgggcc tgttcctcgg cctcgccgct gccgcgacgg
     4561 tgcaggtgca ccgcgtcgac ccgatgtcgt tcctctacct gatgtcggtc gcctgcgtct
     4621 acgactgcgg cgactacctg atcggcagcg ggagccggaa ccggttggtc ggccccgttg
     4681 ccggcctggt gggggtgctc gtcgtggtga ttgcgatgac ggcgatcaac ccgccgccgc
     4741 tgctcgacga cggcgacgtc cgcaagctcg gcgtcgccat ggccctcgcc tgtccgttgg
     4801 gccagctgct cggctcgtgg acgctcccgt cgagtcgggc gaaagcgcca gcgctccgcc
     4861 gcctcgactc gtggctgttg accgccccgg cgtgtctcgt cgggctctgg gtcctgacct
     4921 gaacgaccgc tgtgttggat tgatcgaggt gggtcattcg gctcaggctc aggattcgcg
     4981 aggatgtgcc gatggaaggt gaaccgatgc ccaacccgtc cggtcgacag gagaccccca
     5041 tcgtgatcac cgaactcgac gaattgctgc tcgcgaacga cctcgggtcg ctgcagagcc
     5101 gcccgatcga ggagctgcgc gctctgcgtg accagctcgg tgagatcgaa gccgggctgt
     5161 cgttcgtccg ccgcatggcg caagggcgac tcgacatcgt gctcgcnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnngaga tcatcgggcg gtaccgcagc ggttcggtct
     5341 ccgtcgacga cctcctgaag gggtagcgca gacgtctcgt cggtgactcc ggatgttgtc
     5401 ggcttcgccg acgaacgaac ggtcctgttg ggtcgggccg ccttcggcgt ccctcctgaa
     5461 ggggtagcgc gagccgcgtt cccccgcagg gcgatgatgc cccagccctg ggttgtgccg
     5521 tagcctgatg ccatgaagga cgagatcgac aagcgctggc atgacctcgg tgagttcatc
     5581 cgcgagcagc gcaacgtggg ccagatgtcg ctgcgcaagc tcagcgagtt ggccggcgtg
     5641 tcgaacccct acctgagcca gatcgaacga gggctccgca agccgtccgc cgagatcctg
     5701 cagcagatcg cccgtgccct cgagatctcg tccgagacgt tgtacgtgcg tgccggcatc
     5761 ctcgacgagc ccgacggtgt tccggacctc atcggcgaga tccggcgcga cccgttcatc
     5821 ggcgaagacc agaagaagac gctcgtgcga atctacgagt cgttccgccg cgagaacgac
     5881 gccggcgtcg ccgaagcctg agagcgacgg atcaccacgg gaatctcggg tcgacgtgtg
     5941 ctgttggggt aactcgtgac tgcggtggcg atgctctccc tgcatacctc accgctcgcg
     6001 cagccgggag agggcgacag cggcggtatg aacgtgtacg tccgcgagct ggcggcgtcc
     6061 ctcgcgcagg ccggggtgcc gacctcggtc tacgttcggc actggcggga gggcctggcc
     6121 ccccgtgtgt cggtggagcc gttgttcgag gtcgtgcacg tgccggttgg cccctacgac
     6181 ctcgccaagg agcagttgcc ggcggtcgcc gacgagttcg ccgactgggt gggcgctgac
     6241 atcgaggccc gaggcgacgt gacggtgctg cacgccaact actggctctc cgctgtcgcc
     6301 gcccaccggc tcaagcacga actcgagctg ccgctcgtga ccacgttcca cacgctggcc
     6361 caggtcaaga ccgcgaccgg cgacatcgaa ccgggccagc gggccgaggc cgagcgggtg
     6421 gtcatcgggt gctccgatgc cgtgtgtgcg tccaacgggg tcgaggccga tcagctcatc
     6481 gacttctacg gcgcggatcg cggccgcatc gagatcgttc ctcccggcgt cgaccacgcg
     6541 ttctttgccc ccggcgatcg ccgtggagcc cggtgggccc tgggtctcgg ggcggagccg
     6601 gtgctcctgt tcgtgggccg catccagccg ctcaaggggc tcgacgttgc cgtcgccgcg
     6661 ctgggtgcga tgaggtcgca gacagcgacg ctcgtcgccg tcggtgggcc gtcgggtgcc
     6721 gagggcgacg ccgaactggc gaggatccag gcgatgatcg gcgacctggg cctcgatgcc
     6781 cgggtgcgtc tcgtcgcacc acagccccac cacatgctgt cgacctacta ccgagcggcc
     6841 gacgtctgcg tggttccgag tcggtccgag tcgttcgggc tcgtcgctgc cgaggcgatg
     6901 gcctgcggca caccggtggt cgcgacggcc gtgggcggtc tcacgacgtt ggtcgacgac
     6961 ggcgtcaccg ggctgctcat gcccaaccgt gatccgaacg cgtgggctgc agcgctcgac
     7021 ggcctgatcg agcacccgtt gcgtctcgcg gcgatgtcgg ctgcagcggc cgagcgtggc
     7081 cgcggataca cctggtccac gacggcggcc cgcctccgcc gggtgtactc cgacctgtcg
     7141 cgctcgcggg tcccggcaac gtgtggagtc gtgggatgag catcgacgag gagatgccgg
     7201 cgagcacccc cgaggaactg gcgcgattgg ccgacctgat cgacgcatgg gtgcagcggc
     7261 agatcgacga accctgggtc gcccacgtcg aacggggcga gggctccgag ccccggtggt
     7321 tcatccgggt caacggcgag gagaagtccg tcttctcgat ctggttcatg ctccggcagc
     7381 ggtcgctgtt cgtcgagacc tacgtgctgc ccgcccccga ggagaacgcc gccgcggtct
     7441 atgagtacct gctccgccgc aatctgcgca tcttcggcat gagcttcgcg atcggcgagg
     7501 aagacgcgat ctacctcacg ggtgaggtgc acaaccgcga cgtcaccgac gccgagctcg
     7561 accgtctgct cggcacggcc tacctccaga ccgagcagtg cttccggccg gcgatgcgcc
     7621 tcgcgttcgc ctccaagttc tcgggctgag ccccgccgcc gggcgaatct ctcccaccac
     7681 atttcagatc acctgttgct ttttcatccg gggtgttgtt acgtgtccgc aaggccagag
     7741 ggtgccgcgc cggacgtcgg ggaagcggac cgatgtgagc gccctctggc cttcatgcgg
     7801 cgcacgacgg gttcggtctc gtcggggctg gtcggagcat cgcgttaggg ggcctcagcg
     7861 aggttccggg cgcgggaacg gtacggatac gacccgctgg cgcgcccgga agccgatcgc
     7921 gagcgagcgt ggggctatcg actgacttcc tgggtggccg aacgggtgcc gtaggctcgg
     7981 gccccatgac caggttgcag atcgtcggcg gcggacggat gggagaagcc ctgatcgggg
     8041 gcatcctgtc ctcgggctgg gccgaagcca ccgaccttcg catcgtcgag gcgagcagcg
     8101 cccgccgaga gcagctcgct tcgctcttcc ccggcgccga gatcacgacc gaaccgggtg
     8161 ccgccgatgg cacgatcgtc gcgacgaagc cggccgacat cgccgccgtc acggctgcgg
     8221 tgagcgcttc cgggggcggc cggatcctgt ccatcgccgc cggcgtgacg ctcgcgaccc
     8281 tcgaggccgc ggccgggtcc ggcgtcgccg tcgtccgcgc catgcccaac accccggcgc
     8341 tcatcggtgc cggtgcttct gccatcgccg ggggcgccgc agcggaagac gccgacctcg
     8401 actgggcgga atcgctcctg ggggccgtcg gtgccgtcgt ccgcgtcacc gagaagcagc
     8461 tcgatgccgt caccggcctc tcggggtccg gtccggcgta cgtgttcctg atcgccgagg
     8521 ccatggtcga cgccggtgtc gccgccgggt tgccccgcga cctgtcgacg acgctcgcct
     8581 accagacgct cgtcgggtcg gcccggctgt tggccgaggg cgacgcgtcc cccgccgatc
     8641 ttcgggccgc ggtcacgtca ccgggcggta ccaccgcggc cggcgtgcag gtgctcgagc
     8701 gcaacgcagt gcgggccgcg gtgatcgacg ccgtcctcgc cgcccgcgac cgagcgaccg
     8761 agttgtcggc atcatgagcg agcccgccga gggcgtcgac aacgccgacc cccgtacgta
     8821 cgacaccgtc accctgctga gcgatctcgg caccgccgac ggcttcgtcg gcgtgatcca
     8881 ctccgtgatc cgccagatgg cgccgggcgc cggcgtcgtc gacctcaccc acggcatcgc
     8941 gccgttcgac gtccgggccg ggtccctcgt cctcgcccgc agtgtgcagt tcctgtgccc
     9001 cggcgtgatc atcggcatgg tcgatccgtc cgtcggcacc gagcagcgcg ccatcgctct
     9061 cgaggtggcc ggcggcgccg ctgtgctcct cggccccgac aacgggctcc tggccccggc
     9121 ggtcggaatg atcggcggcg ccgaacgggc cgtggtcctc gacaacaccg acctgcatct
     9181 gcccacggcg tcgacgacct tccccggccg cgacatctac gcccccgtcg ctgcgcacct
     9241 gtgcaacggc gtggcgttcg ccgacctcgg cagcgaggtc gatccgggct cgctgatgcc
     9301 gggcctcgtg cctctcacca gcctcgagca ggatgccgac ggcgtcgacg tgctcggggc
     9361 cgaggtgctc tgggtcgacc acttcggcaa cgtgcagctc aacgtcgatc ccgacgagct
     9421 cgacggctgg ggcgaccgga tccagctgcg catcggcacg aacgtccgca cggccacgcg
     9481 catcacggcg ttcggccagc tcacgaccgg cgagatcggc ctgctcgtcg atgccgacgg
     9541 cctggtcgcc gtcgtcaccg atcgtcaccc ggccgccgag gaactcgggc tcgcaaccgg
     9601 cgacgccgtt gccctcagcc cgatcggcga cgaccaagcg atcggtgtcc gtaccgccgt
     9661 gaccatcgga cgccgcgacc cggcgtcgac ccctccagga ggtgcctcat gaggcgcgga
     9721 accacgatcg tgatcgccct gttgctcatc gtgctggtcg tcgccgcggc gctccagctc
     9781 gggctcagcg ccaaccgctg acgcctcggg ccatcaggtc agagccgtga ggcgacccgg
     9841 tcccgggtga acctcacgag accgccgcgg atcaggagca gcgccagcac ccacacgacg
     9901 ccgccggcca cgaagatcca gtacgggggc atcatcagca ggccactggt ctggccgacc
     9961 gacgcgagga tcaggggcat gatcaccgcg ccgccgagct ggttggcctc ctgggtcgtc
    10021 cgggctcggg cactgaccct gaccatgacc ccgagcccga gcgcggcgac agccggcgcc
    10081 atgaagccga tgagaccgat ccatcgccag gtgggcacga acagtcggcc catcaccggc
    10141 caggcgacga cgttggccga cacggcgaac agcgtgaacc cgatccatga ggccgccacc
    10201 gtcggcagga acgcgaacag caccttcgac acgtagaggt cgcgctcttc cacgggcagg
    10261 tgcaggagcg actcgagcgt gcgtcgctcc ttctcgccgg cgaacgtgtc ggctgcgagg
    10321 gccgagctga tcatcatcgg cacgatcagg aacaacggtg cgatcaggta gccgagcacg
    10381 agctcgatga gctgctggcg ctgggggagg gactgcaggt cctgcgccat cgacgtgggc
    10441 aggtgcgaca tgagaccgct caggtcgggt gccgtgccgg tccgggcgaa gagaccgatg
    10501 ccggtcggca ggcccaccag cagcacgaac ggcacgacca gcatcgggat cacgacggcc
    10561 ttcgagcgtc gcgccgccga gatgtccttt gcgaggagag cccggatcat cgtccagtcg
    10621 aggccggtgg ccggtcggtg ggtgggagcg tccgtcggct ctcgtccgtc gaggtcgagc
    10681 gaacgctcgt cgagcaccgc ggtcatgcgg cacgctccgt ggcgtcggac gaggcggcgt
    10741 cgggacgagc cggtggagtg gtcgcgagcg agcgctgcag gtcgtcctgc acggcgaagt
    10801 acacgtcctc gagccccttc gggcgggcct gggtgccgta cacggttgca ccgagcccga
    10861 cgacgagcgc gacgacggcg gggatgtcct cccgcccgcg cacgttgatg acggcaccgt
    10921 cggcgaccgg catcatcgcc atgacggcgt ggtggtcgag cagtgcctgg ccgactcgca
    10981 gcgacggcga cgcgccgagg tcgaggtgca cttcgaggcc cggccagcgc tcggccgc
//
LOCUS       DAOSOA010000078        10983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5945_length_10983_cov_6.086018, whole genome shotgun sequence.
ACCESSION   DAOSOA010000078 DAOSOA010000000
VERSION     DAOSOA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5945_length_10983_cov_6.086018"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKY13_06895"
     CDS             <1..242
                     /locus_tag="PKY13_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOT79698.1"
                     /translation="LPVSAVAIVYLIGAVVQSVSPTPGGLGPAEAAYIGGLTAIGLSS
                     ERAVAAVLLFRMLTFWLPVIPGWVAMTWLQRNDAL"
     gene            293..1681
                     /locus_tag="PKY13_06900"
     CDS             293..1681
                     /locus_tag="PKY13_06900"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HOT79699.1"
                     /translation="MDQHFDLCIIGTGSGNSILDDRFDDWNVAIVERGTFGGTCLNVG
                     CIPSKMLIYPADIVEHLREGARLGIDASLDHVRWRDIRDRTFGRIDPIAAGGRQYRIG
                     LPNVTVFEEDARFVGPKTLQVGGEQITADRFVLAAGARAHVPPLPGLDLVPYDTSDTI
                     MRIDEVPERLVILGGGFIACEMAHVFDAFGSKVTIVVRGNGLLKQEDVEIAKRITDRL
                     SERMDVIRFATPMNVSMHDGAYLVEVDTPTGLVSVEADRLLVATGRVPNGGQLNVEAT
                     GVRLDPNGYVVTDEHLFTGVDGVWALGDVRNPLQLKHLANLEGRVVQHNLLHPDDLRA
                     IDERFVPHAVFTNPQIGSVGLTEHQAEMHGRPYVTAVKDYAGTAYGWAMEDTTSVCKV
                     IADAETRQLIGAHIIGPQASILIQQLVQGMRFGQTVDQMARDVIYPHPALSEVVENAL
                     LDLVAALDRTAG"
     gene            1684..2403
                     /locus_tag="PKY13_06905"
     CDS             1684..2403
                     /locus_tag="PKY13_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79700.1"
                     /translation="MEKLVYLLWGDAAAGSDHYRDLLVDTVAPELRDRGVAQLTIDVD
                     DAASDVASPVPTPSGEQPHVAAVSVWLPANDRRGPVEEVLASTGLRSAGYLVSGAVYT
                     DYGDNEWGAERTWGAGERSPSVLTVCLIHRPPDADPTEWVRHWHGVQSPVSAEIQPRM
                     RYVRNEVVRPLTDGAPEVHGIVEEAWPSGQHIIDPMLFFNGFGDRDLMNANIGRMIDS
                     VGAFIDMDRMRNVTMSEYLWP"
     gene            2556..4145
                     /locus_tag="PKY13_06910"
     CDS             2556..4145
                     /locus_tag="PKY13_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79701.1"
                     /translation="MSSRLEIELTSSRPDGVWTWRKAGAKMPKGEVEASKLPEGSKVG
                     DILRADADILIDGVDILAVLPPKDARKDKFESLQVVGTRKDEPLVTQTLAPKGRGGGR
                     DRDRGDRGDRPRRDGDRPRRDGDRPRRDGDRPGGDRGPRGAGGDRPRRPAPEPKPRAK
                     RLRPGRTHRNAVLDSLAPEQRPIAEHVLRGGVPAVRQAVDKQNELNKAQGLPEIHGDE
                     LVALAEGLLPKLRSAEWRDKADNALSILAELDLRDLRSVVVAADTGARDDETRALAQQ
                     LRDGLAKRVEEEHTSWLGELSQTLAEGRVVRALRLSSRPPKAGAPVPAEITTKLTEAT
                     LENLTLETSQDRWAMVIDALAFSPVRTAVTPAAVPAEPTDELKAAIAKVASRVPHIAA
                     LFGIDASAAPKRKRPAKAAGTEKRPPAPPKLEAAAEPATDEAPVVEEALADTSTEAPA
                     PEPATDEAPVVEETVAAEETEAPAEEAVAEEPVAAEAPAEETVAAEAPATHAEEAAEG
                     AGDEPVVEPVSDEHAEAPAEG"
     gene            complement(4215..4763)
                     /locus_tag="PKY13_06915"
     CDS             complement(4215..4763)
                     /locus_tag="PKY13_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT79702.1"
                     /translation="MPAYDIVVFDLDGTLIDSDDALVRPFLTLGMAQENITFGHPIEE
                     FCAAVGIDVDEYVRLYDTDIVEPYPGAIELICGLDRWAVCSNKHPESGTAELVRLGWT
                     PEVALFSDAFGGAAKQLAPVLELVGVEAGRTLFVGDTAHDARCAEVVGCDFAWAAWNP
                     RTAATHPAGTILTDPRQVLDLL"
     gene            4841..5614
                     /locus_tag="PKY13_06920"
     CDS             4841..5614
                     /locus_tag="PKY13_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOT79703.1"
                     /translation="MIDFEKIGNIAVITINRPDARNAVNTAVAQGIEDAIDQIESDDE
                     IWAAILTGAKTEKGYIFCAGADLKQMSVDPGGMSTAKGGFGGLVQRERTKPLIAAVDG
                     PALAGGTELVLACDMVVASKTAVFGIPEVKRNLVAAAGGLFRLPRKIPRNIAMELALT
                     GRLDFPAERAYHFGLVNHLTEEGGALDGAKALAAQICEAAPLAVRESRRIVIEATDQP
                     DEIGWKMSGDGIMAMFSTEDFSEGLNAFIEKRSPVWKGR"
     gene            complement(5702..6127)
                     /locus_tag="PKY13_06925"
     CDS             complement(5702..6127)
                     /locus_tag="PKY13_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT79704.1"
                     /translation="MIDELAPAARPEVAVGVIAVQDDALLLIRRGHGPAAGRWSVPSG
                     RIERGETAAEAIVRELQEETGLAGVCGPLIGWAELISDEAHFVVLNFEVIIMDDGEPR
                     AGSDAAEALWVPLDQVEEYRLTPGLAEFLAEHNIITLIV"
     gene            complement(6138..6575)
                     /locus_tag="PKY13_06930"
     CDS             complement(6138..6575)
                     /locus_tag="PKY13_06930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT79705.1"
                     /translation="MVGARVASADDGTAVAELRRLARSELLEQRGGEQFTAAEMAGPS
                     VDVPPSTPDAAVVVGTIGEDVVGVATVLRSGRRARLEEFFTHPGARGVGIGHAMLGEV
                     SRLARSWECTDLDSYALPGDRDTKNFFESHAMKSRLLIVHTAL"
     gene            complement(6576..7397)
                     /locus_tag="PKY13_06935"
     CDS             complement(6576..7397)
                     /locus_tag="PKY13_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79706.1"
                     /translation="MTDSTPSSPLEALLRLPATLAAELGAALPRFADRTRAQVDFAAT
                     IVEKLRCGDLGSRLSSFGAQVPERLADVVPFEPRAAADAAVGLAATVAASSPVREVIE
                     VVTVAASTRSGAPSEPDADEAPVAAAAPAKKAPAKKAAPAKKAPAKKAPAKKAPAKKA
                     PAKKAAPAKKAAPAKQAAPAKKAPAKKAPAKKAPAKKAPVAAAAPAPAPVTEPTGPVL
                     ESTLAIPGYDALAASQVVPRLESLSASDLEQVRSYELAGRGRRTILSRIAQLQAG"
     gene            complement(7518..8039)
                     /locus_tag="PKY13_06940"
     CDS             complement(7518..8039)
                     /locus_tag="PKY13_06940"
                     /inference="COORDINATES: protein motif:HMM:NF025204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HOT79707.1"
                     /translation="MDDDDVIDEEFVVDEDTADAEPDGPEFEDDDDLEADLEEDDELA
                     DPDVIVEVEDEEEDEEPVVPATRARPAGEDEDEDEDPDPDDVEADLDTILKDRIASGA
                     DDDEDEEEEVPVDRNDPEATEGVVSKREGEFTCTGCFMIVHPRQFGRKGRLTCPTGDE
                     DCPSISIVAAQLP"
     gene            complement(8202..9821)
                     /locus_tag="PKY13_06945"
     CDS             complement(8202..9821)
                     /locus_tag="PKY13_06945"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOT79708.1"
                     /translation="MTPYVAEDFSSTEQAILRRYFTNLDGPVFALVNLPEVVKGALFA
                     RYSRSAKSLRRLFIDEFVNDLDVSGDANIDATIGLERAEALYDKVFFEYGDDSVAQLG
                     GVHLACEQASNILTKALEWGRIMSYLEQSTRYIAYDARLGGRYRFFRDPSVLASPLGA
                     RYVGDMDRMFDTYAELVPRLTDFFREAHPKHPDDSDFVYRQAIRAKAFDALRGLLPAA
                     SLSNVGIYGSGQGYEALLLRLKSLQLPEANAYADLMLTELRKVIPSFLKRVDLPDRGG
                     AWSNYLATNNQAMAEVAAKIFPTVGAPEDKSSVTLVDFDPDAEIKTVAAMLYPYCHEP
                     EEQIEDRVREMTVDERITVMKAYVGDRTNRRHKPGRALERSFYRFDVLSDYGAFRDLQ
                     RHRMMTIEWQALTPYHGYIRPDSVDAAGARDMFDETMERSANLYRDLSANFPQQASYA
                     VSLAYRVRYSMQFNSREAMHLLELRSGPQGHPTYRTVAQQMHEQLSEVAGHRALAAMM
                     IYVDHSEESDLERLDAERRAEAKRSAGAGAI"
     gene            complement(9886..>10983)
                     /locus_tag="PKY13_06950"
     CDS             complement(9886..>10983)
                     /locus_tag="PKY13_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT79709.1"
                     /translation="HQIWANPELCFEEHFAHDLLSGVLESSGFAVERRAYGLDTAFVG
                     RFGDPSATGPTIAMLCEYDALPEVGHACGHNIIAAAGLGAALGAAAVVDALGGRLVVL
                     GTPAEEGGGGKIAMMNAGAFEGIDAAFMVHPADADLDSFWAIAIHELHVQYTGKAAHA
                     AAAPEQGRNALDAAVLGYMNVAALRQHIGRSERIHGIITHGGDKPNVVPHRSAMQWYV
                     RSATAESLAALKPRVLAALEAGALATGCEMTAEWKHYPYDDLLTNGPLDGLYAANAAS
                     LGRVVEARETRPSFMGSTDMGNVSHLVPSIHPMIKAAPDGVAIHTAEFATHAGGALGD
                     AAVIDGALAMAHTAVDLWAEPERLDAARALL"
BASE COUNT         1706 a         3866 c         3782 g         1629 t
ORIGIN      
        1 acctgccggt gtcggccgtg gcgatcgtgt acctcatcgg cgccgtggtg cagtcggtct
       61 cgccgacgcc gggcggcctc ggtcccgccg aagccgccta tatcggtggc ctcaccgcga
      121 tcgggttgtc gtccgagcgg gccgtcgccg cggtgctcct cttccgcatg ctcaccttct
      181 ggttgccggt catccccggc tgggtcgcga tgacctggct ccagcgcaac gacgcgctgt
      241 agtgctgcca cgcggtcacc gccggcgcag cgcagtcgta gggtgagcgt ccgtggacca
      301 acacttcgac ctctgcatca tcggcaccgg ctccggcaac agcattctcg atgaccgctt
      361 cgacgactgg aacgtggcga tcgtcgagcg cggcaccttc ggcggcacgt gcctgaacgt
      421 cggatgcatc ccgtccaaga tgctgatcta ccccgccgac atcgtggaac acctccgcga
      481 gggcgcccgc ctcggcatcg acgcgagcct cgatcacgtg cggtggcgcg acatccgcga
      541 tcgcacgttc ggccgcatcg acccgatcgc cgccggcggc cgccagtacc gcatcggcct
      601 cccgaacgtg accgtcttcg aagaagacgc ccggttcgtg gggcccaaga cgctgcaggt
      661 cggcggtgag cagatcacgg cggaccggtt cgtcctcgcc gccggggccc gggcccacgt
      721 gccgccgctg ccggggctcg acctcgtccc ctacgacaca tccgacacga tcatgcggat
      781 cgacgaggtc cccgagcgcc tcgtcatcct cggtggcggg ttcatcgcct gcgagatggc
      841 ccacgtcttc gacgcgttcg gttcgaaggt gaccatcgtc gtgcgcggca acgggctgct
      901 caagcaggag gacgtcgaga tcgcgaagcg catcaccgac cgcctgtccg aacgcatgga
      961 cgtcatccgg ttcgccacgc ccatgaacgt gagcatgcac gacggcgcct acctcgtgga
     1021 ggtcgacaca ccgaccgggc tggtctcggt cgaggccgat cggttgctcg tcgcgaccgg
     1081 tcgggtcccc aacggcggcc aactcaacgt cgaggcgacc ggggtgcgac tcgacccgaa
     1141 cggctacgtc gtcaccgacg agcacctctt caccggcgtc gacggcgtct gggccctcgg
     1201 tgacgtgcgc aacccgctgc agctgaagca cctcgcgaac ctcgagggcc gtgtcgtcca
     1261 gcacaacctg ttgcatcccg acgacctgcg ggccatcgac gaacgcttcg tcccccatgc
     1321 ggtgttcacg aacccacaga tcgggtcggt cgggctcacc gagcaccagg cggagatgca
     1381 cggccgcccc tacgtcaccg cggtcaagga ctacgccggc accgcgtacg gctgggcgat
     1441 ggaggacacg accagcgtgt gcaaggtcat cgccgacgcc gagacccgtc agttgatcgg
     1501 tgcccacatc atcggtccgc aggcgtcgat cctgatccag cagctcgtgc agggcatgcg
     1561 cttcggccag acggtcgacc agatggcccg cgacgtgatc tacccccacc cggcgctgtc
     1621 cgaggtcgtc gagaacgcgc tgctcgacct cgttgccgct ctcgaccgga cggcgggctg
     1681 acgatggaga agctcgtcta cctgctgtgg ggcgatgcgg ccgccggttc ggaccactac
     1741 cgcgacctgc tcgtcgacac ggttgctccc gagctccgcg accgcggcgt cgcccagctg
     1801 acgatcgacg tcgacgacgc cgcgtccgac gtcgcgtccc cggtcccgac gccatcgggc
     1861 gaacagccgc acgtcgcggc ggtgtcggtg tggttgccgg ccaacgatcg ccgcggaccg
     1921 gtcgaggagg tgctggcctc gaccgggctc cggtccgccg ggtacctggt gtcgggtgct
     1981 gtgtacaccg actacggcga caacgagtgg ggcgccgagc gcacgtgggg tgccggggag
     2041 cgatcgccgt cggtgctgac ggtctgcctg atccaccgtc cgcccgacgc cgacccgacc
     2101 gagtgggtgc ggcactggca tggtgtgcag tcgccggtgt ccgccgagat ccagccccgc
     2161 atgcgctacg tgcgcaacga ggtcgtgcga ccgctgaccg acggcgcccc cgaggtgcac
     2221 gggatcgtgg aggaggcgtg gccgtcgggc cagcacatca tcgatccgat gctcttcttc
     2281 aacggcttcg gcgaccgcga cctgatgaac gccaacatcg gccggatgat cgacagcgtc
     2341 ggtgcgttca tcgacatgga ccggatgcgc aacgtgacca tgtccgagta cctctggccc
     2401 tgagcgcccc accaacccga cccgctccac tgccggtttt gtcccgcgga agcgcgacga
     2461 atcgcgctcc ttcgctggac agaacgggcg gcgtcgccgg gagttcagct cccgttgccg
     2521 gggaagcccc cgccacccgt tagattgggt cccctatgtc gtccaggctt gaaatcgaac
     2581 tgaccagctc ccgccccgat ggggtgtgga cctggcgcaa ggcaggggcg aagatgccca
     2641 agggtgaggt cgaggcctcg aaactccccg aaggctcgaa ggtcggtgac atcctgcgag
     2701 ccgacgccga catcctcatc gacggcgtcg acatcttggc ggtcctcccg cccaaggacg
     2761 cccgcaagga caagttcgag tcgcttcagg tcgtcggcac ccgcaaggac gagccgctcg
     2821 tgacccagac cctcgccccc aagggccggg gtggcggccg tgaccgcgat cgtggtgacc
     2881 ggggcgaccg cccccgccgc gacggcgacc gtccgcgccg cgatggcgat cgtccgcgcc
     2941 gcgacggcga ccgtcccggc ggcgaccgtg gccctcgtgg cgccggcggc gatcgcccgc
     3001 gtcgccccgc ccccgagccc aagccccgcg ccaagcgcct ccgtcccggt cgcacccacc
     3061 gcaacgccgt gctcgactcg cttgcgcccg agcagcgccc gatcgccgag cacgtgctcc
     3121 gcggcggtgt cccggcagtc cgccaggccg tcgacaagca gaacgagctc aacaaggctc
     3181 agggcctccc cgagatccac ggtgacgaac tcgtcgccct ggccgagggt ttgctcccca
     3241 agctccgttc cgccgagtgg cgcgacaagg ccgacaacgc gttgagcatc ctcgccgagc
     3301 tcgatctccg cgacctccgc tcggtcgtcg tggccgccga caccggcgcc cgtgacgacg
     3361 agacccgtgc gctcgctcag cagctccgtg acgggctcgc caagcgtgtc gaggaggagc
     3421 acacctcctg gctcggcgag ctgagccaga ccctcgccga gggtcgggtc gtccgcgccc
     3481 tgcgcctgag ctcccggccg cccaaggccg gtgcgcccgt gcccgccgag atcacgacga
     3541 agctcaccga ggcgaccctt gagaacctca cgctcgagac ctcccaggat cgctgggcga
     3601 tggtcatcga cgcactggcg ttctcgccgg tgcgcacggc cgtgaccccg gccgcggtgc
     3661 cggccgagcc gaccgacgag ctgaaggccg cgatcgccaa ggtcgcgtcc cgcgtgccgc
     3721 acatcgcggc gctgttcggc atcgacgcca gcgctgcccc gaagcgcaag cgcccggcga
     3781 aggcggccgg caccgagaag cgcccgccgg ccccgccgaa gctcgaggcc gccgctgaac
     3841 cggcgactga cgaggcgccc gtggtcgagg aggcactcgc cgacacgtcg accgaagctc
     3901 ccgctcctga gcccgcgacc gacgaagcgc ccgtggtcga ggagaccgtg gctgccgagg
     3961 agaccgaggc acccgccgag gaagcagtcg ccgaggaacc cgtggctgcc gaggcacctg
     4021 ccgaggagac cgtggctgcc gaggcacccg ccacgcacgc cgaggaagca gcggaaggcg
     4081 ccggcgacga accggtcgtc gagcccgtgt ccgacgagca cgctgaagcc cccgccgagg
     4141 gctgaccgac cacctctcgc ggttttgccc tgcagatcga ccggattgcc gattgaactg
     4201 cagggcaaaa tccttcagag caggtcgagc acctgacgag ggtccgtcag gatggttccc
     4261 gccgggtggg tcgcagccgt tcgcgggttc caggccgccc acgcgaagtc gcatccgacc
     4321 acctcggcgc accgcgcgtc gtgtgccgta tcgccgacga agagcgtccg cccggcctcg
     4381 accccaacga gctcgagcac cggcgccagc tgttttgcag cgccaccgaa cgcgtcggag
     4441 aagagcgcga cctcgggcgt ccagcccagg cgcaccagct cagcggtgcc cgactcggga
     4501 tgcttgttgg agcacacggc ccagcgatcg agcccgcaga tcagctcgat cgccccggga
     4561 tacggctcga cgatgtcggt gtcgtagagc cggacgtact cgtccacatc gatcccgacc
     4621 gccgcacaga actcctcgat cgggtgcccg aaggtgatgt tctcctgtgc catcccgagc
     4681 gtcaggaagg gtcgaacgag cgcgtcgtcg gagtcgatga gggtaccgtc gaggtcgaag
     4741 acgacgatgt cgtaggcggg catgcggcgt tcctagcagg ggtcaccggc gcccggtcaa
     4801 gaagtagacc gtccggtcaa gttccaatag ggtgaccgcc atgatcgact tcgagaagat
     4861 cggcaacatc gccgtcatca ccatcaaccg ccccgacgcc cgcaacgccg tcaacacggc
     4921 cgtcgcccag ggcatcgagg acgccatcga ccagatcgag tccgacgacg agatctgggc
     4981 cgccatcctc accggcgcga agaccgagaa gggctacatc ttctgcgccg gcgccgatct
     5041 caagcagatg agtgtcgacc cgggcggcat gagcaccgcc aagggcggct tcggcggtct
     5101 cgtgcagcgc gagcgcacca agcccctcat cgccgcggtc gacggacccg ccctcgccgg
     5161 cggcaccgag ctcgtcctgg cgtgcgacat ggtcgtggcc tcgaagaccg cggtgttcgg
     5221 aatccccgag gtgaagcgca acctcgtcgc tgctgccggt ggcctcttcc gcctcccccg
     5281 caagatcccc cgcaacatcg cgatggagct cgcgctcacg ggtcgcctcg acttcccggc
     5341 cgagcgtgcg taccacttcg gcctggtcaa ccacctcacc gaggagggcg gggcgctcga
     5401 cggcgccaag gccctggcgg cgcagatctg cgaggccgcc ccgctggccg tgcgcgagag
     5461 ccgtcggatc gtcatcgagg ccaccgacca gcccgacgag atcggttgga agatgtcggg
     5521 tgacggcatc atggcgatgt tctcgaccga ggacttctcc gagggcctca acgccttcat
     5581 cgagaagcgc tcccccgtct ggaagggccg ctgaccctcg acctcaccgc atttgtcctg
     5641 cggaatcgcc cctcgagggg cgattccgca ggacagaacg ggtgacgaga acgggtgacg
     5701 ctcagacgat gagcgtgatg atgttgtgct cggcgaggaa ctcggcgagg cccggagtca
     5761 accggtactc ctcgacctga tcgagtggga cccagagggc ctcggccgcg tcgctcccgg
     5821 cgcggggctc gccgtcgtcc atgatgatca cctcgaaatt gaggacgacg aagtgggcct
     5881 cgtcgctgat cagctcggcc cacccgatga gcgggccgca cacgccggcg agaccggttt
     5941 cctcctggag ctcgcggacg atcgcctccg ccgcggtctc ccctcgctcg atgcgcccgc
     6001 tgggaaccga ccagcgccca gcagccggac cgtgaccgcg gcggatgagc agcagcgcgt
     6061 cgtcctgcac ggcgatgacg ccgaccgcca cctccgggcg agctgctggg gccaactcgt
     6121 cgatcaacga accgtcctca caacgccgtg tgcacgatca ggagccgcga cttcatggcg
     6181 tgactctcga agaagttctt ggtgtcacgg tcaccgggga gcgcgtagga gtcgaggtcg
     6241 gtgcactccc agctccgggc cagacggctg acctcgccga gcatggcatg gccgatgccg
     6301 acgcctcggg caccgggatg ggtgaagaac tcctccagac gggcacgccg accggaacgg
     6361 aggaccgtgg cgacgccgac gacgtcctcg ccgatcgttc cgacgaccac cgcggcgtcg
     6421 ggcgtcgagg gtgggacatc gacggacggg ccggccatct cggcggcggt gaactgctca
     6481 ccgccgcgct gctcgaggag ttcggagcgt gcgagacgac ggagctccgc cacggcggtg
     6541 ccgtcatcgg cggacgcgac gcgagctccg accactcaac cggcctgcaa ctgggcgatg
     6601 cggctgagga tggttcgacg accgcgcccc gcgagttcgt aggagcggac ctgctcgagg
     6661 tcggacgccg acaacgactc gagacgaggg acgacctgcg acgcggccag cgcgtcgtag
     6721 ccggggatgg cgagcgtcga ctcgagcacg ggcccggtcg gttccgtcac cggggcgggc
     6781 gccggtgcag cagcggcgac tggcgccttc ttggccggag ccttcttcgc cggagctttc
     6841 ttggcgggag ctttcttcgc cggagcggcc tgcttggcgg gagccgcctt cttggcggga
     6901 gccgccttct tcgccggcgc cttctttgcc ggcgccttct tcgccggcgc cttcttcgct
     6961 ggcgccttct tggcgggagc cgccttcttg gcgggggcct tctttgccgg ggccgccgca
     7021 gcgacgggcg cctcatcggc gtcgggctcg gagggagctc ccgaacgagt ggacgcggcg
     7081 acggtcacga cctcgatcac ttcacgcacc ggcgacgacg ccgcgacggt ggcagcgaga
     7141 ccgactgcag catcggcggc cgcccgcggt tcgaacggca cgacgtcggc cagccgctcg
     7201 gggacctgcg cgccgaacga gctgaggcgg gacccgaggt cgccgcaccg cagcttctcc
     7261 acgatcgtcg ccgcgaagtc gacctgggca cgagtgcggt cagcgaagcg cggcaacgcc
     7321 gccccgagct cggcagcgag ggtggcggga aggcgcagca acgcctccaa cggggaactg
     7381 ggcgtggagt cggtcacggc agggatcatt gccgcgccac ccccggcgcg gcaacccagc
     7441 cgagccggcc ggagcggtcc ggcagcgtca gccggccgac gatcgcggcc agccctcggc
     7501 gagggtggcc gcgacggtca cggcagctgc gccgcgacga tcgagatcga cgggcaatcc
     7561 tcgtcgccgg tcgggcacgt gagtcgaccc ttgcggccga actgacgcgg gtggacgatc
     7621 atgaaacacc cggtgcacgt gaactcgccc tcacgcttcg acacgacgcc ctcggtggcc
     7681 tcggggtcgt tgcggtcgac ggggacctct tcttcctcgt cctcgtcgtc gtcggcgccg
     7741 gacgcgatgc gatccttgag gatcgtgtcc aggtcggcct cgacatcgtc ggggtcgggg
     7801 tcctcgtcct cgtcctcgtc ctcgccggca ggacgggcgc gggtggcggg aacgaccggc
     7861 tcctcgtcct cctcctcgtc ctcgacctcg acgatgacat cgggatcggc gagttcgtcg
     7921 tcctcctcga ggtcggcctc cagatcgtcg tcgtcctcga attcggggcc gtccggctcg
     7981 gcatctgccg tgtcctcgtc caccacgaac tcctcgtcga tcacgtcgtc atcgtccacg
     8041 aattgcttcc ctcgccatgc tgtcttgcgg tcgtgcggcg ctgatcatag acacacttcc
     8101 gcccgacacg tggacttgcc ggcccgggga cctcgcaacg acgaggcggc cgacggcgag
     8161 ggggacggcg ttcacgaccc gcccgaccgg gtcgacggag atcagatcgc gccggcgccg
     8221 gcgctgcgtt tcgcctcggc ccgacgctcg gcgtcgagac gctcgaggtc gctctcctcg
     8281 gagtggtcga cgtagatcat catggccgca agggcgcggt gcccagcgac ctccgacagc
     8341 tgctcgtgca tctgctgggc gacggtccgg taggtcgggt ggccctgcgg accggaacgg
     8401 agctccaaca ggtgcatggc ctcacggctg ttgaactgca tggaatagcg cacgcggtac
     8461 gcgagcgaca cggcatacga ggcctgctgg gggaagttcg ccgacaggtc gcggtacagg
     8521 ttggccgacc gctccatcgt ctcgtcgaac atgtcgcggg caccggcggc gtcgaccgag
     8581 tcgggacgga tgtacccgtg gtagggcgtg agcgcctgcc actcgatggt catcatgcgg
     8641 tggcgctgga gatcgcggaa cgcgccgtaa tcgctcagca cgtcgaaccg gtagaacgac
     8701 cgctcgaggg cacgaccggg cttgtggcgc cggttcgtgc ggtcacccac atatgccttc
     8761 atgaccgtga tccgctcgtc gaccgtcatc tcgcggacgc ggtcctcgat ctgctcctcc
     8821 ggctcgtggc agtaggggta gagcatcgcc gccacggtct tgatctcggc gtcgggatcg
     8881 aagtcgacca gcgtcaccga cgacttgtcc tcgggcgctc ccaccgtcgg gaagatcttc
     8941 gcggccacct cggccatggc ctggttgttg gtcgccaggt agttgctcca ggcgccgccg
     9001 cgatcgggaa ggtcgacgcg cttcaggaac gacggaatga ccttgcggag ctcggtgagc
     9061 atgaggtcgg cgtaggcgtt ggcctcgggg agctgcaacg acttgaggcg caggagcagt
     9121 gcctcgtagc cctgacccga gccgtagata ccgacgttcg acagcgaggc tgccggcagc
     9181 aacccccgca atgcgtcgaa cgccttggcc cggatcgcct ggcggtagac gaaatcgctg
     9241 tcgtcggggt gcttggggtg ggcctcgcgg aagaagtcgg tgaggcgcgg gacgagctcg
     9301 gcgtacgtgt cgaacatgcg gtccatgtcg cccacgtacc gggcgccgag cggcgacgcc
     9361 aggaccgacg ggtcacggaa gaaccggtaa cggccaccga gccgggcgtc gtacgcgatg
     9421 tagcgcgtcg actgctccag gtagctcatg atccggcccc actcgagagc cttggtcaag
     9481 atgttcgacg cctgctcgca ggcgaggtgc acaccgccga gctgggcgac cgaatcgtcg
     9541 ccgtactcga agaacacctt gtcgtagagc gcctcggcac gttcgaggcc gatggtcgcg
     9601 tcgatgttgg cgtcgccgga cacgtcgaga tcgttgacga actcgtcgat gaacaggcgg
     9661 cgcagcgact tcgcggatcg cgagtagcgc gcgaacaagg cgcccttcac gacctcgggg
     9721 aggttcacca gtgcgaacac gggcccgtcg aggttggtga agtaccgccg aaggatcgcc
     9781 tgctcggtac tgctgaagtc ttcggcgacg tagggggtca cggcgttcga ggttaggcgt
     9841 ccggccgggt gggtacgggg atcgtcggcg cagtcggttc ggaggtcaca gcaacgcccg
     9901 cgccgcgtcg agccgctcgg gctcggccca caggtcgacg gcggtgtgtg ccatggcgag
     9961 agcgccgtcg atgacggcgg catcgccgag cgctcccccg gcgtgagtgg cgaactcggc
    10021 ggtgtggatc gccacgccat cgggcgccgc cttgatcatc gggtggatcg acgggaccag
    10081 gtggctcacg ttgcccatgt cggtcgagcc catgaacgag ggtcgggtct cacgggcctc
    10141 gacgaccctc ccgagcgacg cggcgttggc cgcgtagagc ccgtcgagag ggccgttggt
    10201 gagcaggtcg tcgtaggggt agtgcttcca ctccgcagtc atctcgcagc cggtggcgag
    10261 cgctccggcc tcgagggccg cgagcacccg gggcttgagg gcggccaggg actccgcggt
    10321 cgctgagcgc acataccact gcatggccga ccggtgcggc accacgttcg gcttgtcgcc
    10381 gccgtgggtg atgatgccgt ggatgcgctc ggagcgaccg atgtgctgac gcagcgcggc
    10441 cacgttcatg tacccgagga cggcggcgtc gagtgcgttg cggccctgct cgggcgccgc
    10501 cgcggcgtgc gccgccttgc cggtgtactg cacgtgcagt tcgtggatcg cgatcgccca
    10561 gaacgagtcg aggtcggcat cggcggggtg caccatgaac gcggcgtcga tgccctcgaa
    10621 cgcgccggca ttcatcatcg cgatcttgcc gccgccgccc tcctcggccg gcgtccccag
    10681 gacgacgagc cggccgccga gcgcgtcgac gacggcagcc gcaccgagcg ccgcgcccag
    10741 gcccgccgcg gcgatgatgt tgtgaccgca cgcgtggccg acctcgggaa gcgcgtcgta
    10801 ctcgcagagc atcgcgatgg tgggcccggt ggccgacggg tcgccgaacc ggccgacgaa
    10861 cgcagtgtcg agcccgtacg cacgccgttc gacggcgaag cccgacgact cgagcacacc
    10921 cgacagcagg tcgtgggcga agtgctcctc gaagcacagc tcggggttcg cccagatctg
    10981 gtg
//
LOCUS       DAOSOA010000079        10964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5959_length_10964_cov_6.254377, whole genome shotgun sequence.
ACCESSION   DAOSOA010000079 DAOSOA010000000
VERSION     DAOSOA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5959_length_10964_cov_6.254377"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKY13_06955"
     CDS             complement(<1..458)
                     /locus_tag="PKY13_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT79710.1"
                     /translation="MTVFSSVLDMIGNTPMVDVSRLSPNPNVRILGKMEGQNPGGSVK
                     DRIARQMILEAEADGTLYPGATLIEPSSGNTGIALAMICQERGYHIKILLPESVSIER
                     RQMLEVFGAEIMLTPGGEGSNGAVRRAIDLAAENPDWVFLYQYGNEANPRA"
     gene            complement(550..963)
                     /locus_tag="PKY13_06960"
     CDS             complement(550..963)
                     /locus_tag="PKY13_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HOT79711.1"
                     /translation="MLQLTSAVHTAMLAHAYDGLPDEACGLMVGSLGDGHSATVDTFE
                     PCPNVAGSSRIYSIAPKDFMRIERAADDAGADIVGVMHSHTHTEPYPSVTDVNQAPDP
                     SWHYVIVSLRDELPMLRSYRIVDGGITEEAVVVSG"
     gene            1031..1519
                     /gene="smpB"
                     /locus_tag="PKY13_06965"
     CDS             1031..1519
                     /gene="smpB"
                     /locus_tag="PKY13_06965"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOT79712.1"
                     /translation="MAEKSETPEIKNIATNRAARHNYTLTDTFEAGIVLRGSEVKSLR
                     ESKVQIVDAFAQLSNGELWLHNLHIAPYSFSQAYSGHDPLRVRKLLLNRREIEKIRRK
                     QLADRLNIVPLRIYFKDGKAKVEIALGKGKTKGDRRQDLAKKDAERDVRNAMGRALKY
                     GN"
     gene            1643..2365
                     /locus_tag="PKY13_06970"
     CDS             1643..2365
                     /locus_tag="PKY13_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79713.1"
                     /translation="MVVALIVGACAPPPSPPATSVRGEPSVRRPVVIIHGWQFFCGFE
                     DAETWKAWIDDAVARGYERDDVSVFSYDTCQPSATTIADFGSFVDGVLARTGAPKVNV
                     IAHSMGAIEARSCIRFGACAGKVDKFASIAGANHGTVWANVCELAFWSKSTCDMKPDG
                     PFLANLNAGDETWGDVEYVSMISWCDLTIVPFTGAALDGALNIVTDRCLSHTDWRGDT
                     VAADWVLDWFDGAAPATAPLTS"
     gene            complement(2461..3885)
                     /locus_tag="PKY13_06975"
     CDS             complement(2461..3885)
                     /locus_tag="PKY13_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79714.1"
                     /translation="MASNTSSVAATCGMAADILSAVEPVRALVGGLDPRNVPLPWAPE
                     VLAAFVEMERVAASGRLLMTGRAAEAGAWERERFASAADWLANQQGTTAGRARADLET
                     SERLADLDATSEAVREGRLSPEQACAVADAAAVNPDAERDLLDRAQRDSLRRTRQEAE
                     RRKAEVRSEAEKAEREARVRNERSARFWYSNGAGHLEVRGPVAEMKELEQRIQREVDR
                     KFRANRAPAQRSSRANYAFDALIEMGNKVADAKRAPLTRMTLIRVDLAALTRGHTEGA
                     ELCEIGGVSVSVGELRRLLGDSVLQLVLTNGEAVIDVVNLARKPTVAQMLAKLFTDSC
                     CTAEGCDRAARLEFDHRDDWARVQVTELANLDLLCDHHHDLKTLEGWQLVSGTGRRPM
                     VPPGDARHPGHVPDDEPEPVVPIVVSDLDATPMPVEADAEPVSRLGSDARVADLAARL
                     ARIRQKEAERRARRLGQDRLFDAG"
     gene            4051..4438
                     /gene="ssrA"
                     /locus_tag="PKY13_06980"
     tmRNA           4051..4438
                     /gene="ssrA"
                     /locus_tag="PKY13_06980"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4491..5762)
                     /locus_tag="PKY13_06985"
     CDS             complement(4491..5762)
                     /locus_tag="PKY13_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT79715.1"
                     /translation="MPTTSDVEAALTRLAREESGRVLALLARRFRDLDLADEAVQDAL
                     TTAARTWPVDGIPTNPPAWLFTVARNRAIDRLRRDASARRRTLAAAPDLINADESTTS
                     DSSGGLDMIDERDGQTIAEDQLRLMLLCCHPALDRDAQVALTLRLVGGLTTTEIAAGL
                     LVQEPTLAQRIVRAKRKIRDAGIPLTIPVDLAERVDVVLGVLYLIFNEGYLPRSTDTV
                     VRIDLVDEAIRLTQALADLLDDEPEVLGLLALEHFHRARSGTRTDHAGDLILLEDQDR
                     TRWDGPEIDEANGVLLRAIRQMRPGPYQLQAVIARHHAIARTAADTDWPAIVAAYGQL
                     SAMTDSPVVRLNHAVAIAMADGPHAGLAALELVDGLENYHLVHAVRGELLARAGRPDD
                     ATEAFTAAIARTTNEAERRHLTRRRAATRPA"
     gene            complement(5769..6131)
                     /locus_tag="PKY13_06990"
     CDS             complement(5769..6131)
                     /locus_tag="PKY13_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOT79716.1"
                     /translation="MEYMILLYSDESFEMPAPDTAEFGEFMGEWMAYNQKLIDGGHWV
                     AGASLQPTATATTVTKAFGAVPSITDGPYVETKEQLGGYYLISAEDLDEAIALAKEVP
                     IPVGSFEVRPIAFRPDAA"
     gene            complement(6260..6781)
                     /locus_tag="PKY13_06995"
     CDS             complement(6260..6781)
                     /locus_tag="PKY13_06995"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT79717.1"
                     /translation="MSSNSNPNNSSKSPLSVTVSAAIAAPADVVYGLISDVTRMPEFS
                     PENIRGEWLDGATGPTVGARFKGTNKLGSTTWSTKPTVTAAEPGRRFAFKVPGSSGAL
                     WTYELASQDGVTVVTESMVQQKASSPLIRWFQRRAGVTDRAANLRSAMTTTLERVAVA
                     AQCQAVADCPIDV"
     gene            complement(6926..8080)
                     /locus_tag="PKY13_07000"
     CDS             complement(6926..8080)
                     /locus_tag="PKY13_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT79718.1"
                     /translation="MAGTTGAEPIRYGIIGTGMMGCEHILNLGHIDDAVVVAISDPNA
                     PSLEMAQACIDVGPSGRRSERLGVYSDHAQLLARDDLDAVVIASPNHTHADVLADALA
                     SDVHVMVEKPVCTTVPDALRVRSLAAGRSSGGPPLMCWVALEYRYMPTPAMLLDLVRS
                     SLLGTTRMVAIREHRFPFLTKVGNWNRFTANTGGTLVEKCCHFFDLMHQLAGDRPTRV
                     MASGGQDVNHLDELVELPDGSTARPDILDNAFVIVEFRNGVRGSLDLCMFAEGGRYEQ
                     ELTVVGDLGKLETTVPGDTVWHGERPAVGSSPSGAVAVAAPLADEVRYTGFHHGSSFR
                     EHLRFIEQIRSGGDALVTVDDGLWSVAMGAAAHRAIEEGRVVDLAEWDLP"
     gene            8100..9032
                     /locus_tag="PKY13_07005"
     CDS             8100..9032
                     /locus_tag="PKY13_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT79719.1"
                     /translation="MLDLPPIPPTRMLGSSGIEVGSIAYGCWRLAGTGVADASAKIGA
                     ALDAGLTLIDTADIYGFGGGGGERDDGFGEAEALLGRVLRDTPEWRDRMVLASKGGIR
                     PGVPYDQSRDYLAEACDASLRRLGVDHLDLYQIHRPDHLTSPAEVAGALDALVDAGKV
                     RAIGVSNFTVAQHRALAAHLRAPIVTTQPEWHPLHQAPLVDGTLDLCGEVGCTPLVWS
                     PLAGGRLVGDPASLGPDAARVAAVADRIAADRGVDRSAVLLAWIAHHPSGAIPIVGSQ
                     RPDRVTASAQALDVRMGRQEWYEILVAGRGERLP"
     gene            9029..10117
                     /locus_tag="PKY13_07010"
     CDS             9029..10117
                     /locus_tag="PKY13_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT79720.1"
                     /translation="MTEPSTTPGTEIAWFSALCDDDYRWLGVPDPTLRSSFDHCADIV
                     TTAERVGFDNVLLPSGYDLGIDSVAFAGGVAPMTDRIRLLVAVRCGELWPPQLARQLA
                     TLDQMLQGRLTINIISSDLPGAPIDGGPRYARSREVMTILRTLLRGEPLDHHGEFYDL
                     VLDPPRVASDAAQARGGTAPPFYFGGMSEPARDTAAEHADVFLMWPDTMDGVQSTISD
                     MRARADAHGRSLRFGYRVHVVVRETEDAARAAAFDLVSRLEPEVGAAVRQRSLDASSV
                     GVARQTDLRDRADADGYVEANLWTGIGRARSGCGAAIVGDPDQVVAKLREYEALGIAS
                     FILSGYPHREECELVGRHVLPAIRGRTT"
     gene            10141..>10964
                     /locus_tag="PKY13_07015"
     CDS             10141..>10964
                     /locus_tag="PKY13_07015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT79721.1"
                     /translation="MGAGIAGLTAARSLVESGAGVIVVDKSRGVGGRMATRRIGDAVL
                     DHGAQFVTARTDWFRAEIQAWEHAGVATPWFEGTLGADGITASDGPARHRGVPAMTGI
                     AKHLATGLDVRLSTHVDRIHHADGRWVIEISDRFPIEADAIVLTAPAPQTLAMLDPAS
                     IDSSDRTRLEQIGYDPCIAVLAVLDGPSGIGHPAARRPAAGPVAWYADNAGKGISAVP
                     ALTVHATAAASRALWELDDATVVTDLLAGIDLAGVHVVDSHVHRWRFARSAELLPEA"
BASE COUNT         1695 a         3794 c         3730 g         1745 t
ORIGIN      
        1 gcgcgagggt tggcctcgtt gccgtactgg tagaggaaca cccagtcggg gttctcggcg
       61 gccaggtcga tggctcggcg gacggcgccg ttggagccct cgccgccggg ggtgagcatg
      121 atctcggcgc cgaacacctc gagcatctgc cggcgctcga tcgacaccga ctcgggcagc
      181 aggatcttga tgtggtagcc gcgctcctgg cagatcatcg ccagcgcgat accggtgttg
      241 cccgacgacg gctcgatgag cgtggcgccc gggtagaggg tgccgtcggc ctcggcctcg
      301 aggatcatct ggcgggcgat gcggtccttg accgagccac cggggttctg gccctccatc
      361 ttgccgagga tccgaacgtt cggattgggg ctgaggcgtg agacgtcgac catcggcgtg
      421 ttgccgatca tgtcgagcac ggaggagaag acggtcatcg aggttccaga acgttggagg
      481 agccaggtca attcccgacc tgttcggtaa ggattctact gcagtgacag tacccagctc
      541 gaaatcggct cagccggaaa cgaccacggc ctcctcggtg atcccgccgt cgacgatgcg
      601 gtacgagcgc agcatcggga gttcgtcgcg gagcgacacg atcacgtagt gccacgacgg
      661 gtcgggggcc tggttcacgt cggtgaccga cgggtacggc tcggtgtggg tgtggctgtg
      721 catcaccccg acgatgtcgg cgccggcatc gtcggccgcc cgttcgatgc gcatgaagtc
      781 cttcggtgcg atcgagtaga tgcgcgacga cccggccacg ttggggcacg gttcgaacgt
      841 gtcgacggtt gccgagtggc cgtcgccgag gctgccgacc atcagcccgc acgcctcgtc
      901 gggcagcccg tcgtaggcgt gggccagcat cgcggtgtgg acggcggagg tcagctgcag
      961 cacgcctgaa cgttaggccg ccgaaagccc ggcgggcgcc gaccccgcct tcggtagcat
     1021 cgaacccgcc atggccgaga agagcgagac ccccgagatc aagaacatcg ccacgaatcg
     1081 ggcggcccgt cacaactaca cgctgacgga cacgttcgag gccggcatcg tgctccgtgg
     1141 cagcgaggtg aagtcgctcc gcgaatcgaa ggtccagatc gtcgacgcgt tcgcccagct
     1201 gtcgaacggc gagctctggc tgcacaacct ccacatcgcc ccgtactcgt tcagccaggc
     1261 gtactccggt cacgatccgc tccgggtccg caagctgctc ctgaatcgcc gtgagatcga
     1321 gaagatccgc cgcaagcagc tcgccgaccg gctcaacatc gtgccccttc gcatctactt
     1381 caaggacggc aaggcgaagg tcgagatcgc actcggcaag ggcaagacca agggcgaccg
     1441 ccgccaggac ctcgccaaga aggacgccga gcgcgacgtc cgcaatgcca tgggccgcgc
     1501 cctcaagtac ggcaactgag tcgatcgccc acggggatct ccagattcga tctggcatcc
     1561 cccgatgccg acacggcaac ctgtcccgat gcggctgtgg gagttgggga cacgacggac
     1621 tcgagccggg gcggtgctga cgatggtcgt cgccctgatc gtgggggcgt gcgctccccc
     1681 gccgtcgccg ccggccacca gtgtccgcgg cgagccgtcg gttcgtcggc ccgtcgtcat
     1741 catccacggg tggcagttct tctgcgggtt cgaggacgcc gagacgtgga aggcgtggat
     1801 cgacgacgct gttgcccgcg gttacgaacg tgacgacgtc agcgtcttct cgtacgacac
     1861 gtgtcagccc agcgccacca cgatcgcgga cttcgggagt ttcgtcgacg gggtcctcgc
     1921 ccggaccggt gcaccgaagg tcaacgtgat cgcacactcg atgggggcga tcgaagcccg
     1981 ctcgtgcatc cgcttcggtg cgtgcgccgg gaaggtcgac aagttcgcgt cgatcgccgg
     2041 cgccaaccac ggcaccgtgt gggccaacgt gtgcgagctc gcgttctggt cgaagtcgac
     2101 ctgcgacatg aagcccgacg gcccgttcct cgcgaacctc aacgccggcg acgagacgtg
     2161 gggtgacgtc gagtacgtct cgatgatctc gtggtgcgac ctcacgatcg tgcccttcac
     2221 gggcgcagca ctcgacgggg cgctcaacat cgtgaccgac cgctgcctct cccacaccga
     2281 ttggcgcggc gacacagtcg cagccgactg ggtgctcgac tggttcgatg gggccgcgcc
     2341 ggcgacggca ccgctcacgt cctgacggtc gcgccgaggc ggcgggctga gcccgcttcg
     2401 cccactttgc cccgtcctca cagtcgcccg ccgccaccca acccgccacg ccggcgtcga
     2461 tcaaccggcg tcgaacaggc ggtcctgccc caacctccgg gcacgacgct ccgcctcctt
     2521 ctgcctgatc cgggcgaggc gggcggcgag atcggcgacg cgagcatcgg acccgagccg
     2581 cgatactggc tctgcgtcgg cttcgaccgg catcggggtc gcatcgagat ccgacacgac
     2641 gatcgggacc accggctccg gctcgtcatc cggtacgtgg ccaggatgtc gagcgtcgcc
     2701 cggtgggacc atcggccgcc ggccggtgcc cgagacgagc tgccagccct cgagcgtctt
     2761 gagatcgtgg tgatggtcgc agagcaggtc gaggttcgcg agttcggtga cctggacccg
     2821 cgcccagtcg tcacgatggt cgaactccaa ccgtgccgcc cggtcgcacc cctcggccgt
     2881 gcagcacgaa tcggtgaaga gctttgccag catctgcgcc accgtcggct tccgcgccag
     2941 gttcacgacg tcgatcacgg cctcgccgtt cgtgagcacg agttgcagca ccgagtcacc
     3001 caagagtcgt cgcagttcac cgaccgacac cgacaccccg ccgatctcac acaactctgc
     3061 gccctcggtg tgcccacgcg tgagcgcggc gagatccacc cggatcaacg tcatgcgggt
     3121 cagcggcgct cgtttcgcgt cggcgacctt gttgcccatc tcgatgagcg cgtcgaacgc
     3181 gtagttcgca cgcgagctcc gttgtgccgg tgctcggttg gcgcggaact tccggtcgac
     3241 ctcgcgctgg atccgctgct ccaactcctt catctccgca accgggccac gcacctcgag
     3301 gtgccccgcc ccgttcgagt accagaaccg cgccgaccgc tcgtttcgaa cccgagcctc
     3361 acgctccgcc ttctcggcct cggaccgcac ctcggccttg cggcgttccg cctcctgccg
     3421 agtgcgtcgc agactgtcgc gctgcgcccg gtccaacagg tctcgctcgg cgtcagggtt
     3481 caccgctgcc gcgtcggcca ccgcacaggc ctgctccggc gacagccgac cttcccgcac
     3541 cgcctccgat gtcgcgtcca gatctgccag tcgttccgac gtctccagat cggcccgagc
     3601 acgaccggcc gtcgtgccct gctggttcgc caaccagtcc gccgccgacg cgaaccgctc
     3661 ccgttcccac gcccccgcct ccgcagcccg acccgtcatc aagagtcgac ccgacgctgc
     3721 gacccgctcc atctcgacga acgcggccaa cacctcgggc gcccacggca acggcacgtt
     3781 ccgcgggtcg agacccccga ccaacgcacg caccggctcg accgccgaca agatgtcggc
     3841 cgccatgcca cacgttgctg caaccgaact ggtgttcgaa gccatgtgac cattcaagca
     3901 tcgaccaccg acagtcaggc tccgaatcgg tcccgttggg aacaatcacc caacgccgaa
     3961 ctcgctgacg acgacgccgc cggacccgct acggtggagt cccgatcatc cgaggctcca
     4021 tccggaccac acaagaagga cgatcaccga ggggctgact ggtttcgacg ttcggaccgg
     4081 aagcttgttc atgcgacccg agttgctcag actcgctaaa cggggcaacc aaaagaagcg
     4141 ccaacagcga cttcgctctc gccgcctgat ctaaccatta ggtagtagag agacatcgtg
     4201 actcgtaaga gcgcacgggg ccagaccact acagcccggc aacagaagta gtggtggacg
     4261 gcagggagag accgctggcg gtgtgaaaga acactgactt cgaggaaaga ctcggcggtg
     4321 ggttccggcc caatcgaccc ggcgacgtgg cagccgtaga gccacaaccg cgggaatggt
     4381 cgtagcaggt caagtgaccg ccgagcggac gggagttcga ttctccccag ctccaccagg
     4441 gcgtgccgag aggttcgtga ggacgtgatc ctggtcgctc ccgccagccg tcaagccggc
     4501 ctcgttgcag ctcggcgtct ggtgaggtgg cggcgttcgg cctcgttggt agtacgggcg
     4561 atggctgcgg tgaacgcttc ggtggcgtcg tcgggtcgtc ctgctcgggc gaggagctcg
     4621 cctcggacgg cgtggacgag gtggtagttc tcgagcccgt cgacgagctc gagtgcagcg
     4681 agcccggcgt gggggccatc ggccatcgcg atcgcgactg cgtggttcag gcggacgacg
     4741 ggagagtcgg tcatcgccga gagctgaccg taggcggcaa cgatcgccgg ccaatcggtg
     4801 tcggccgcgg tgcgggcgat cgcgtggtgg cgggcgatga cggcctggag ttgatagggg
     4861 ccgggtcgca tctggcggat ggcccggagc aggacgccgt tggcctcgtc gatctcaggg
     4921 ccgtcccatc gagtccggtc ctggtcctcg agaaggatca ggtctccggc gtggtcggtg
     4981 cgcgtgcctg agcgagctcg gtggaagtgc tcgagcgcga ggaggccgag gacctcgggc
     5041 tcgtcatcga ggaggtcggc gagggcttgg gtgagccgga tcgcttcgtc gacgaggtcg
     5101 atgcgaacga ccgtgtcggt agagcgaggg aggtacccct cgttgaagat gaggtagagg
     5161 actccgagga cgacatcgac gcgttcggcg agatcgaccg ggatggtgag cgggatcccg
     5221 gcgtcgcgga tcttgcgttt tgctcgaacg attcgttgcg cgagggtcgg ctcctgcacg
     5281 agaaggccgg ctgcgatctc cgtggtggtg agtccgccga cgaggcgcaa ggtcagggcg
     5341 acctgcgcgt cccgatcgag ggcgggatgg cagcacagca gcatgaggcg gagctggtcc
     5401 tcggcgatgg tctggccgtc gcgttcgtcg atcatgtcga gtcctccgct cgagtccgag
     5461 gtcgtggact cgtcggcgtt gatgaggtcg ggcgcggcgg cgagggtacg acgtcgggcg
     5521 gaggcgtccc ggcggaggcg gtcgatcgct cggttgcggg cgacggtgaa gagccaggcg
     5581 ggagggttcg tcgggatacc gtcgacgggc catgtgcgag cggccgtggt gagagcgtcc
     5641 tgcacggctt cgtcggccag gtcgaggtcg cggaaacggc gcgcgaggag agcgagcact
     5701 cgcccgcttt cctcgcgtgc cagccgggtg agggctgcct cgacgtcgct ggtcgtcggc
     5761 actgcccctc aggcggcgtc ggggcgaaag gcgatcggtc gcacctcgaa cgagccgacg
     5821 gggatcggca cctccttggc gagtgcgatc gcctcgtcga ggtcctcggc cgagatgagg
     5881 tagtagccgc cgagctgttc cttggtctcc acgtacgggc cgtcggtgat cgacggcacg
     5941 gcaccgaagg ccttcgtgac cgtcgtggcg gtcgcggtcg gttgaaggct ggcgccggcc
     6001 acccagtggc ctccgtcgat cagcttctgg ttgtacgcca tccactcgcc catgaactcg
     6061 ccgaactcgg cggtgtcggg ggcgggcatc tcgaagctct cgtcgctgta gagcaggatc
     6121 atgtattcca tggttctcgt tgtacctttc gttgtcgttg aatgtcgggt tgggatggga
     6181 actggtctcg agccgcgccg atgaaccggt catcggcgaa ggaacggccc ggacagcgaa
     6241 ctggcgctcg gtctgggtgt cagacgtcga tggggcagtc tgcgacggcc tggcattggg
     6301 cggccacagc aacccgctcg agcgtggtgg tcatcgccga tcgcaggttc gccgcgcggt
     6361 cggtcacgcc ggcccgacgc tggaaccagc ggatcagcgg ggacgaggcc ttctgctgga
     6421 ccatcgactc ggtcaccacg gtcacgccgt cctggctggc gagttcgtag gtccacagcg
     6481 ccccgctgga accgggcact ttgaacgcga atctccggcc cggctcggcg gcagtgacgg
     6541 tcggcttcgt cgaccaggtc gtgctaccca gcttgttcgt gcccttgaac cgggcgccga
     6601 ccgtcgggcc ggtcgctccg tcgagccact cgccacggat gttctccggg ctgaactctg
     6661 gcatccgcgt cacgtcgctg atcaggccgt acaccacatc cgcaggggca gcaatcgcgg
     6721 ccgacacggt cacactcagc ggcgacttgg acgagttgtt cgggttcgag ttgctgctca
     6781 tctggctctc cgtgtgctct tgcagcgatg ccctgtgcgt cgctggcaca tggatgacgc
     6841 agcaccccgg acgaatcgac agccccgtcg aagaaatctc gagaaatctc tttccaccct
     6901 gaggtcggca gcctcgtgat gtccgtcagg gcaggtccca ctccgccagg tcgacgacgc
     6961 ggccctcctc gatcgcccga tgcgccgctg cgcccatggc gacggaccac aacccgtcgt
     7021 cgaccgtcac gagcgcatcg ccgccggacc ggatctgctc gatgaaccgg agatgttccc
     7081 ggaagctgga gccgtggtgg aacccggtgt agcgaacctc gtcggcgagc ggggcagcga
     7141 ccgcgacggc gccgctcgga gacgacccga ccgcggggcg ttctccgtgc cacaccgtgt
     7201 cgcccggcac cgtcgtctcg agcttgccga gatcgccgac gacggtgagt tcctgctcgt
     7261 accgcccgcc ttcggcgaac atgcacagat cgagcgaccc gcgaaccccg ttgcggaact
     7321 ccacgatcac gaacgcgttg tccaagatgt cgggccgcgc cgtcgaaccg tccggtagct
     7381 cgacgagttc gtcgaggtgg ttgacgtcct gtcctcccga cgccatcacc cgggtggggc
     7441 ggtcgccggc gagctgatgc atcagatcga agaagtgaca gcacttctcg acgagcgtcc
     7501 cgccggtgtt ggcagtgaac cggttccagt tcccgacctt ggtgaggaac gggaagcgat
     7561 gttcgcggat cgcaaccatc ctggtggtgc ccaagaggct cgacctgacc agatcgagca
     7621 gcatcgcagg ggtcggcatg tatcggtact ccaacgccac ccaacacatg agcggcggcc
     7681 cgcccgagga tcgcccggcc gcgagcgacc ggacacgcag ggcatccgga acggtcgtgc
     7741 acaccggctt ctcgaccatg acgtgcacgt cggaggcgag ggcgtcggcg agcacatcgg
     7801 cgtgggtgtg gttcggtgag gcgatgacga cggcgtcgag gtcgtcgcgg gccaggagct
     7861 gggcgtgatc gctgtagacg cccagacgtt cagagcgacg gcctgacggc ccgacgtcga
     7921 tgcacgcctg cgccatctcg agcgacggtg cgttcgggtc gctgatggcg acgacgaccg
     7981 cgtcgtcgat gtggccgagg ttcaggatgt gttcgcaccc catcatcccg gtgccgatga
     8041 ttccgtaccg aatcggctcg gctccggtcg ttcctgccat ctccggtacc gtaccggctg
     8101 tgctcgacct gcccccgatc ccaccgacga ggatgctcgg atcgagcggc atcgaggtcg
     8161 gctccatcgc ctacggctgc tggcggttgg cgggcaccgg tgtcgccgac gcctccgcca
     8221 agatcggcgc ggcgctcgac gccggcctca ccctgatcga cacggccgac atctacggct
     8281 tcggcggtgg aggcggcgaa cgcgacgacg ggttcggcga ggccgaggcg ttgctcggcc
     8341 gcgtgctccg cgacaccccc gaatggcggg accgcatggt gctcgcctcc aagggcggca
     8401 tccgccccgg cgtgccctac gaccagtccc gcgactacct cgccgaggcg tgcgacgcct
     8461 cgctccgtcg gctcggcgtc gatcacctcg acctctacca gatccaccgt cccgaccatc
     8521 tcacgtcgcc ggcggaggtc gccggggcac tggacgcgct ggtcgacgcc ggcaaggtcc
     8581 gcgccatcgg cgtctcgaac ttcacggtgg cccaacaccg tgccctggcg gcgcaccttc
     8641 gggcgcccat cgtcacgaca caaccggagt ggcacccgct ccaccaggcg ccgctggtcg
     8701 acggaacgct cgacctgtgc ggtgaagtcg gctgcacgcc gctggtgtgg agcccgctcg
     8761 ccggcggacg tctcgtcggc gacccggcgt ccctcgggcc cgatgccgcc cgggtggcag
     8821 ccgtcgccga tcgaatcgca gcggaccgcg gcgtcgaccg ctcggcggtc ctgctcgcat
     8881 ggatcgccca ccacccgtcg ggtgcgatcc ccatcgtcgg gtcgcagcga cccgatcggg
     8941 tcaccgcgtc ggcgcaggcg ctcgacgtcc gcatggggcg ccaggagtgg tacgagatcc
     9001 tcgtcgccgg gcggggcgag cggctgccgt gaccgaaccg tcgacgacac ccggcaccga
     9061 gatcgcctgg ttctccgcct tgtgcgacga cgactaccgg tggctcggtg tccccgatcc
     9121 cacgctgcgg tcgtcgttcg atcattgcgc cgacatcgtg accaccgccg aacgcgtcgg
     9181 gttcgacaac gtcctgcttc cttcgggcta cgacctcggc atcgactccg tcgccttcgc
     9241 cggcggggtc gccccgatga ccgaccgcat ccgcctgctg gtcgccgtcc ggtgcggtga
     9301 gctctggccc ccgcagctcg cccgccagct cgcgaccctc gaccagatgt tgcaggggcg
     9361 cctcacgatc aacatcatct cgtccgacct gcccggcgcg cccatcgacg ggggcccgcg
     9421 ctacgcccgc agccgcgagg tcatgacaat cctgcggacg ctgcttcgtg gcgaaccgct
     9481 cgaccaccac ggcgagttct acgacctggt gctcgacccg ccacgggtgg cgagcgatgc
     9541 agcgcaggcc cggggaggga cggcgccgcc cttctacttc ggcggaatgt cggaacccgc
     9601 ccgcgacact gcggcggagc atgccgatgt cttcctcatg tggcccgaca ccatggacgg
     9661 cgtgcagtcc acgatcagcg acatgcgggc ccgcgccgac gcccacggcc gctcgctgcg
     9721 gttcggctac cgcgtgcacg tcgtcgtccg cgagaccgag gacgccgccc gggccgccgc
     9781 cttcgacctc gtctcccgcc tcgaacccga ggtcggagcg gccgtgcggc agcggtcgct
     9841 cgacgcgtca tcggtcgggg tcgcccgcca gaccgacctc cgtgaccgag ccgacgccga
     9901 cggctacgtc gaggccaacc tgtggacggg catcggccga gcccggagcg gctgcggcgc
     9961 cgccatcgtc ggcgaccccg accaggtggt cgcgaagctc cgcgagtacg aagcgctcgg
    10021 catcgcctcc ttcatcctgt cgggttaccc ccaccgcgag gagtgcgaac tggtgggtcg
    10081 ccacgtcctg ccggcgattc ggggtcgaac cacgtgatcg gtcggcccgt cgttgtggtc
    10141 gtgggcgccg gtatcgcggg cctgacggcg gcgcgttcgc tggtcgagtc gggcgccggc
    10201 gtcatcgtgg tcgacaagag ccgaggggtc ggcggacgga tggcgacccg tcggatcggc
    10261 gacgcggtcc tcgaccacgg cgcccagttc gtcaccgccc gcaccgactg gtttcgtgcc
    10321 gagatccagg cctgggagca cgccggcgtc gccaccccat ggttcgaagg gaccctcggc
    10381 gccgacggca tcaccgcgtc cgacggcccg gcccgccatc gcggcgtgcc ggcgatgacg
    10441 ggcatcgcca agcacctcgc gaccgggctc gatgtccgcc tgtcgaccca cgtagatcgg
    10501 atccaccacg ccgacgggcg gtgggtgatc gagatctccg acaggttccc gatcgaggcg
    10561 gacgccatcg tcctgaccgc gccggcgcca cagacgctgg cgatgctcga ccccgcgtcg
    10621 atcgattcgt cggaccgcac tcgcctcgag cagatcggat acgacccgtg catcgcggtg
    10681 ctcgccgttc tcgacggtcc gtcgggcatc ggccatcccg cagcacgacg ccccgccgcg
    10741 ggcccggtcg cgtggtacgc cgacaacgcg ggcaagggca tctcggcggt cccggcactg
    10801 acggtccacg ccaccgcggc cgccagccgg gcgctgtggg agctcgacga cgccacagtg
    10861 gtcaccgacc tgctcgccgg aatcgaccta gccggagtgc acgtggtcga ctcccacgtc
    10921 caccgctggc ggttcgcccg atcagcggaa ctgcttcccg aggc
//
LOCUS       DAOSOA010000080        10917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_5996_length_10917_cov_4.945682, whole genome shotgun sequence.
ACCESSION   DAOSOA010000080 DAOSOA010000000
VERSION     DAOSOA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5996_length_10917_cov_4.945682"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKY13_07020"
     rRNA            complement(<1..315)
                     /locus_tag="PKY13_07020"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            986..3388
                     /locus_tag="PKY13_07025"
     CDS             986..3388
                     /locus_tag="PKY13_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOT79722.1"
                     /translation="MSVTSRPSRWAPALLASCLVLGACGFQGRKLPTINAAASGDRTR
                     IFAADGTLITELASEERRQSLPLDQIPRILQNAVVAIEDERFWEHNGVDPKGILRAAG
                     KTSAAGEVAQGGSTITQQYVKNALLSPEQDIQRKIQEASLAIQLERTHTKGFILEQYL
                     NTIWFGNRSYGVQQASKGYFGHDVQGVTIAEAALLAGIIQSPSRFDPYKHLDAATTRR
                     NTVLGKMLELGYITQEEHDEAEAAPITLGDSTTTEVQQRYPAAHFVEEVKRFIRSDPR
                     FGDTEEERNNLLINGGLSIYTTVDLAMQAKAEEDVKKVYPDQNRPLTDRRKDPDIGLV
                     SIEARTGYVRAMVGGYDYFDTDTKVHPYAQYNLAVGKGRQAGSTFKPIVLAAALASGI
                     KPNATFPAPSSTVIKVAGQPNWSVKGHGLGPKANLTECVVHSDNVCFANLIADKRVGV
                     DRATEFAGKMGVDITPWDPSTRTGFKRVLSLVLGANDSTVLDMTEAYTVFANRGLHVP
                     ATLVTKVVDANGTVLYQHQHTQEKVIEPEASDAITQMMEGVLTRGTAAGKGIGRPAAG
                     KTGTTQDETDAWFIGYTPDLVTGVWAGYSETSKRKVGSAGATAAAPVWQMFMKDALAD
                     RPAVDFDFSTNPVATTTTVPAPNTSIFETAATPTMVTMPEVGSGNVNDAVAKLKKVGL
                     VVQRIDIANPPGVNNGQVLGQSPAAGSSVPKGATVTIEATPGNPPPTGPIPDVAGLPA
                     AEAVAGLEKLGYVVTQTPAPAPAGFLLPNGLAPTPGIVWSIAPAVGTVSPDGKLSVNI
                     QP"
     gene            3431..4030
                     /locus_tag="PKY13_07030"
     CDS             3431..4030
                     /locus_tag="PKY13_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79723.1"
                     /translation="MPKRSPVTDEADRDREAVLPADVVSGESKLPPQVRTWGVRFLIL
                     AGLASTLLWVGAIVYGAVGDPHLAGWLEDRTFPTAAEPICKAALDDVNAFPPAHESPS
                     PAARADVIRASTARLQTMLDDLRVEVPDTADAKWINMWLDDWGVHLDDRLDFARRLDE
                     NGAKEEFFESTKENTQISKSLNGFAETNKMESCSTPGDV"
     gene            complement(4154..4774)
                     /locus_tag="PKY13_07035"
     CDS             complement(4154..4774)
                     /locus_tag="PKY13_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT79724.1"
                     /translation="MAVPGAIMTGVFLTAGAPELDRATDVAEALAAIAAVPFGDDALE
                     RDRILMTHLLDGRADAAVRAARPGHLTGSAFVVDSSAERALLLFHTKLQRWLQPGGHA
                     DGDTNLAAVALKEATEESGIEGLRVWSDPIDLDIHRVAPPKEDPHLHLDLRYLVVAPP
                     GAEPAGNEESQALRWVPEAELVTYDVDAGLLRLARSAFTLARSLPA"
     gene            complement(4750..5325)
                     /locus_tag="PKY13_07040"
     CDS             complement(4750..5325)
                     /locus_tag="PKY13_07040"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOT79725.1"
                     /translation="MRRVPAKLLLDHSSVVAPALLGKVLRVDLGEGDVRSGRITEVEA
                     YGGLDDPASHSARGETPRNRPMFGAAGTVYVYFTYGMHWCANIVTGAVGDPQAVLLRA
                     VEPIEGLDAIRAARPRARRDRDLANGPAKLCEALGIGQWANGVALGADVGGRSIRLLD
                     DGTSPPAVPLVGPRVGISVAQEIPWRFRVPS"
     gene            complement(5322..6737)
                     /gene="argH"
                     /locus_tag="PKY13_07045"
     CDS             complement(5322..6737)
                     /gene="argH"
                     /locus_tag="PKY13_07045"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT79726.1"
                     /translation="MSDENEHTAQPLWHGRFAEGPAAELWEYTLSLPFDQRLARFDIR
                     GCRAHVAGLGVAGILSASEVGAVEAALDTTEAELASGEFTYVETDEDIHTAIERRVTE
                     LAGDAGARLHTGRSRNDQVATAFRLFCKDAVATVAERVVALQDVLVARADEVGDAYLP
                     GYTHLQQAQPVLLRHHLLAHFWSFSRDLDRLVDAHRRLDVSPLGAGALAGSSLPIDPI
                     ANAERLGFASAFANSLDAVSDRDFVAEILFDFTMIALHLSRIGEEIVVWSTEEFGFVR
                     LSDAYSTGSSMLPQKKNPDIAELARGKAGRAIGNLTGLLATLKALPLAYNRDLQEDKE
                     PLFDSFDQVRRGLGAVAGMLETITFNTDRMAASADSPYAAATDLAEYLVRGGMPFRDA
                     HAVVGAIVRRSMDEGVELAPLVEAEPTLGPEAAALMRPGVPVRMRTTAGGAGPVPVET
                     QLVAARVRLADDRARAGSLTR"
     gene            6772..7707
                     /locus_tag="PKY13_07050"
     CDS             6772..7707
                     /locus_tag="PKY13_07050"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOT79727.1"
                     /translation="MRLIPFGTSSGRPTPSRNVSGLGVDLGREWILVDCGEGTQQQVM
                     RSPLKLSRLSAILITHLHGDHVLGLPGLLGTMGMEGRTAPLQLVGVDGIGRWLDVMCE
                     LPILGIDFPITITELVDDTLAGAAGEPLHVADVGGFAVSALALRHRVPAFGYRIVEPT
                     RRGHLDVEAARALGVTDGPDLGLLQRGETVPGRTGPVHPDQVVGPDRAGRIVTVLGDT
                     TRCAASVSLARDADLVVHECTYANRDAALGEQWKHSTPADAAWVATRAGARRLLINHF
                     SSRYDDPNELITEARADFPDTIAAQELEPVDIPLR"
     gene            complement(7792..9003)
                     /locus_tag="PKY13_07055"
     CDS             complement(7792..9003)
                     /locus_tag="PKY13_07055"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT79728.1"
                     /translation="MAKKVVLAYSGGLDTSVAVRWMIENLGCEVICVSANVGQEGTLD
                     GNREKALGAGASEYIELDLREEFARDYLAPVIKANALYEGKYPLVSALSRPLIVKHQV
                     EVARRFGADGIAHGCTGKGNDQVRFEVSTMALAPDLELVAPVRNWGFTREDSIAYAAQ
                     HNIPIRATKEKMYSIDDNLWGRAIECGEMEDPWAEPPSGVWELTKPTATERREVVIGF
                     EAGVPVSVDGARMPLHEIVVHLNGVVGAYGWGRLDMVENRRVGIKSRETYEAPGGLAL
                     MLAHADLESITVERDLGREKMKLEHRYAELIYDGLWFSPLKQALDAFIESSQQYVTGE
                     VRLALTPGVDGAGGQCFVVGRRAPKSLYDYGLATYDAADRFRHADSEGFVQLWGLSVK
                     TWSSIQGTGSA"
     gene            complement(9037..9507)
                     /gene="argR"
                     /locus_tag="PKY13_07060"
     CDS             complement(9037..9507)
                     /gene="argR"
                     /locus_tag="PKY13_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOT79729.1"
                     /translation="MTPRISKTARQHRITKLLEENQVTNQTQLVELLAEAGVEVNQAT
                     VSRDLEELSAVKVRVPGGETAYAIPELPFDQVSTDDHLRRVLGDWVAGLDRSGDLVVL
                     RTPPGCAHVVASAIDRSGTPDIIGTVAGDDTLLVIAAQGSGAAVEAAISDLANL"
     gene            complement(9504..10433)
                     /gene="argF"
                     /locus_tag="PKY13_07065"
     CDS             complement(9504..10433)
                     /gene="argF"
                     /locus_tag="PKY13_07065"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOT79730.1"
                     /translation="MPRHFLEIDDLTPAELVDVLDLAESTDLPLALHRRGVALLFEKP
                     SARTRHSMEIATIELGGHPVYVRPQEVGLDVRESTEDVTRTLMGYHAAIAARVFEHSK
                     LERMAAVSSVPIINLLSDDAHPIQVLADLLTIRQELGRLDGVTVAYVGDANNMARSLA
                     LGLAMCGATLRIAHPAGYAFNDAAVARLAAAGVSVDTTGDPHEAAKDADVVYTDAWYS
                     MGQEAEMVQRRRDFAGFQVDADVMGVASPDAVFLHCLPAHRGDEASDDVLDGPQARIW
                     PQAHNRLHSARGLLAWLFLAADDPGAPAVVEES"
     gene            complement(10558..>10917)
                     /locus_tag="PKY13_07070"
     CDS             complement(10558..>10917)
                     /locus_tag="PKY13_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT79731.1"
                     /translation="HATTYGGQPLAAATARKVLEIMVREDVPARSAAAGAAIAEGLVG
                     RPGIAGVRGKGLLLAVELDGVAAGEAAAFLLSRGVVVNAVSPTALRLAPSLLITDDEV
                     KLAVAAIIGAVGELAGV"
BASE COUNT         1788 a         3860 c         3657 g         1612 t
ORIGIN      
        1 gctgatcgtc ctctcagacc agctacccgt cgatgccttg gtgggccgtt accccaccaa
       61 ctagctgata ggccgcgaag ccctcccgaa gcggaatcca tttcctcacc aaaccatgcg
      121 gtccggtggg ggtatccggt attagctctc ctttcggagg gttatcccgg tcttcggggc
      181 aggttcctca cgtgttactc acccgttcgc cactaggcct tccgcagtgt tgccactgca
      241 tgggcctcgt tcgacttgca tgtgttaggc acgccgccag cgttcgtcct gagccaggat
      301 caaactctcc atcgagatca gaaacgcccc cgaccgaagt cagaggcgag acaaatcaga
      361 gagctgggtt ggttctggcg aaccttccca actggcacag ttcgaatcat cactgacgtg
      421 atatttccga attatgcatt aaatgtattg agggtcgttc gaggtgcgct ttcccgaggg
      481 aaagctccat tcacgtcgaa cgcccgcact agcttttgtg cgtcctctct tccgttgtca
      541 aggagcgcgt cgccggcatg cgaatgccga ctgactccac cggaactcgt ttccgctcgt
      601 ctccgagtcg aaatcgttgg ttccgtttgc cccggctgcg gaccgcttga agcggcgccc
      661 cgaggcgaag aagcacgata gtcgtgcctg gaagccgtgt caacttcttc cggaagaatt
      721 tctgcgccga agcgcaaccg gggaagttgg cgtggtgcgg gatccgagga tcctgctccc
      781 gactgcccga cgtactctcg tgccggcatg gtgtccggcg gagtgtgcgt cgctgggaag
      841 cactgtagcg cgtcccccac gcactacgtc aactcggacg acatcggccc gaaatcctgc
      901 cgaaaccttg gccgtcgggg catggaggca gcgcccggcc gtggtgccac cagatcaggc
      961 cgaccgggaa caagaatggg ccgcgatgtc tgtcacctcc cgacccagcc gctgggcgcc
     1021 tgcgctgctc gcctcgtgtc tcgtcctggg cgcctgcggc ttccaggggc gcaagctccc
     1081 caccatcaac gcagccgcct cgggcgaccg cacccggatc ttcgccgccg acggcaccct
     1141 catcaccgag ctcgccagcg aggaacggcg gcagtccctc ccactcgacc agattccccg
     1201 gatcctccag aacgccgtcg tggcgatcga ggacgagcgc ttctgggaac acaacggcgt
     1261 cgaccccaag ggcatcctcc gcgccgctgg caagacgtcc gctgccggcg aggtcgccca
     1321 gggcggctcg accatcaccc agcagtacgt gaagaacgca ctcctcagtc ccgagcagga
     1381 catccaacgg aagatccagg aggcgagcct cgccatccag ctcgagcgca cccacaccaa
     1441 ggggttcatc ctcgagcagt acctgaacac gatctggttc ggcaaccggt cctatggggt
     1501 gcagcaggcg tcgaaggggt acttcggtca cgacgtccag ggcgtgacga tcgctgaggc
     1561 ggcgctgctc gccggcatca tccagtcccc gagccgcttc gacccctaca agcacctcga
     1621 tgccgccacg acccgccgca acacggtgtt gggcaagatg ctcgagctcg gctacatcac
     1681 ccaggaggag cacgacgagg ccgaggccgc gccgatcacc ctcggcgaca gcacgaccac
     1741 cgaggtgcaa cagcgctacc ccgccgccca cttcgtcgag gaggtcaagc gcttcatccg
     1801 ctccgacccc cggttcggcg acaccgagga ggagcgcaac aacctgctca tcaacggtgg
     1861 cctctcgatc tacaccaccg tcgacctcgc catgcaggcc aaggccgaag aggacgtgaa
     1921 gaaggtctac cccgaccaga accggccgct gaccgaccgc cgcaaggacc ccgacatcgg
     1981 cctcgtgtcg atcgaggccc gtaccggtta cgtccgggcc atggtgggtg ggtacgacta
     2041 cttcgacacc gacaccaagg tgcaccccta cgcccagtac aacctggcgg tcggcaaggg
     2101 tcgccaggca ggttccacgt tcaagccgat cgtcctcgca gcggcactcg ccagcgggat
     2161 caagcccaac gcgacgttcc cggccccgtc gtcgacggtc atcaaggtcg ccggccaacc
     2221 caattggagc gtcaagggcc acggcctcgg accgaaggcc aacctgaccg agtgcgtcgt
     2281 gcactccgac aacgtgtgct tcgccaacct catcgccgac aagcgggtcg gcgtcgaccg
     2341 ggccaccgag ttcgccggaa agatgggcgt cgacatcacc ccgtgggacc cgtcgacgcg
     2401 gaccggcttc aagcgggtcc tgtcgctcgt gctcggagcc aacgactcca cggtcctcga
     2461 catgaccgag gcgtacaccg tgttcgccaa ccggggcctc cacgtcccgg cgaccctcgt
     2521 caccaaggtc gtcgacgcca acggcacggt gctctaccag caccagcaca cccaggagaa
     2581 ggtcatcgag cccgaggcca gcgacgccat cacccagatg atggaaggcg tcctcacgcg
     2641 gggcactgcg gcgggcaagg gcatcggtcg gcctgctgcg ggcaagaccg gcaccaccca
     2701 ggacgagacc gacgcgtggt tcatcggata cacccccgac ctcgtcaccg gcgtctgggc
     2761 cggatactcc gagacctcca agcggaaggt cggctcggcg ggggccacgg ccgccgcccc
     2821 ggtgtggcag atgttcatga aggatgcgct cgccgatcgt ccggcggtcg acttcgactt
     2881 cagcaccaac cccgtcgcca ccacgacgac cgtgccggca ccgaacacgt cgatcttcga
     2941 gacggccgcg accccgacga tggtcaccat gcccgaggtg gggtccggca acgtgaacga
     3001 cgccgtcgcc aagctcaaga aggtgggcct cgtggtccag cggatcgaca tcgccaaccc
     3061 gccgggcgtc aacaacgggc aggtgttggg ccagtccccc gccgccggct cctcggtccc
     3121 caagggagcg accgtgacca tcgaggccac gccgggcaac ccgcccccga ccggcccgat
     3181 tcccgacgtc gccggcctcc ccgccgccga ggccgtcgcc ggcctcgaga aactcggcta
     3241 cgtcgtcacc cagactccag ctcccgctcc cgccgggttc ctcctcccga acggtcttgc
     3301 ccccacgccc ggcatcgtct ggtcgatcgc cccggctgtc gggacggtgt caccggatgg
     3361 caagctgtcg gtcaacatcc agccctgacc gcggccctcg ccgcgtccgc cgccgatcga
     3421 tggtgcaccc atgcccaagc gttctcccgt gaccgacgaa gccgaccgcg accgtgaggc
     3481 cgtgctcccc gccgacgtcg tcagcgggga gtcgaagctg ccgccgcagg tacgcacctg
     3541 gggcgtgcgg ttcctgatcc tcgccggcct cgcctccacg ctgttgtggg tcggcgccat
     3601 cgtctacggc gccgtcggcg atccgcacct ggccgggtgg ttggaggatc gcacgttccc
     3661 cacggctgcc gagccgatct gcaaggccgc gctcgacgac gtcaacgcct tccctccggc
     3721 ccacgagtcc ccgagccccg cagcccgggc cgacgtgatc cgggcgtcga ccgcccgctt
     3781 gcagacgatg ctcgacgacc tgcgtgtcga ggtccccgac accgccgacg ccaagtggat
     3841 caacatgtgg ctcgacgact ggggtgtcca cctcgacgat cgtctcgact tcgcccggcg
     3901 cctcgacgag aacggcgcca aggaggagtt cttcgagtcg accaaggaga acacccagat
     3961 ctccaagtcg ctcaacggct tcgccgagac caacaagatg gagtcctgca gcacccccgg
     4021 cgacgtctga ccgccgcgcc tccacccgaa cctccgactg gagcgctgag gtgacacagg
     4081 ccccccgctg gagcgctgag gtgacagaaa ctgtcaccgg agcgctccag agcaactgag
     4141 cgctccggac gggtcaggcc gggagggatc gcgccagggt gaaggccgac cgggcgaggc
     4201 ggaggagccc ggcgtcgacg tcgtaggtga ccagctcggc ctccgggacc caccggagcg
     4261 cctggctctc ctcgttgccc gccggctcgg ccccgggcgg cgccaccacc aggtagcgca
     4321 ggtcgagatg caggtgggga tcctccttcg gaggagcgac ccggtggatg tcgagatcga
     4381 tggggtcgga ccacacccgc aggccctcga tcccggactc ctcggtggcc tccttcagcg
     4441 cgacggcggc gaggttggtg tcgccatcgg catggccacc cggctgcagc cagcgctgga
     4501 gtttcgtgtg gaacagcagg agggcccgct ctgcggacga gtcgacgacg aaggccgatc
     4561 cggtcaggtg ccccggtcgg gcagctcgca ccgcagcatc ggcgcggccg tcgagcaggt
     4621 gggtcatcag gatgcggtcg cgttcgagcg catcgtcccc gaacggcacc gcagcgatcg
     4681 ccgccagcgc ctcggcgaca tcggtcgccc gatccagttc aggggcacca gcggtcagga
     4741 acacgcccgt catgatggca cccggaaccg ccacgggatc tcctgcgcca ccgagatgcc
     4801 gacccggggg cccacgaggg gaacggccgg gggcgacgtg ccgtcgtcca acagccggat
     4861 cgaccggccc ccgacgtcgg ctccgagcgc caccccgttc gcccattgcc cgatgccgag
     4921 ggcctcgcac agcttggcgg gaccgttcgc gagatcacgg tctcggcggg cacgagggcg
     4981 ggcggcccgg atcgcgtcga gcccctcgat cggctccacc gcccgcagga gcaccgcctg
     5041 aggatcgccg accgcgccgg tcacgatgtt ggcgcaccag tgcatcccgt aggtgaaata
     5101 cacgtacacg gtgcccgccg cgccgaacat cggccggttg cgtggagtct cgccgcgcgc
     5161 cgagtgcgat gccgggtcgt cgagaccgcc gtacgcctcg acctcggtga tccggccgga
     5221 acgaacgtca ccctcgccca gatcgacccg gaggaccttt ccgagcagcg ccggcgctac
     5281 caccgacgaa tggtcgagga gcagcttcgc gggaacgcgt ctcaacgcgt gagcgacccg
     5341 gcccgagcac gatcgtcggc gagacggacc cgcgctgcga cgagctgggt ctcgaccggc
     5401 accgggcccg caccgcccgc cgtcgtccgc atcctcaccg ggacaccagg gcgcatcaag
     5461 gctgcggcct cggggccgag ggtcggctcg gcctcgacca gcggcgccag ctcgacgccc
     5521 tcgtccatcg aacggcgcac gatggccccc acgacggcgt gggcgtcccg gaagggcatg
     5581 ccaccacgca ccaggtactc ggccaggtcg gtcgccgcgg cgtacggact gtcagccgac
     5641 gccgccatgc ggtcggtgtt gaacgtgatc gtttcgagca tgccggcaac ggcacccaga
     5701 ccccgacgga cctggtcgaa gctgtcgaac aacggctcct tgtcctcctg caggtcgcgg
     5761 ttgtacgcga gcggcaacgc cttgagcgtg gccaacagcc cggtgaggtt gccgatcgcc
     5821 cgccccgctt tgccccgcgc cagctcggcg atgtcggggt tcttcttctg cggcagcatc
     5881 gacgagcccg tcgagtaggc gtcggacagt cggacgaagc cgaactcctc ggtcgaccac
     5941 accacgatct cctcgccgat gcggctcagg tgcagcgcga tcatcgtgaa gtcgaacagg
     6001 atctcggcca cgaaatcgcg gtcactgacg gcgtcgagcg agttggcgaa cgccgaggcg
     6061 aacccgaggc gctcggcgtt ggcgatcggg tcgatgggga ggctggagcc ggcgagcgcc
     6121 cccgcaccga gcggcgacac gtcgagccga cgatgcgcgt cgacgaggcg gtcgaggtcg
     6181 cggctgaacg accagaagtg ggccagcagg tggtggcgca gcagcaccgg ctgggcctgc
     6241 tgcagatggg tgtagccggg caggtaggcg tcgccaacct cgtcggcccg agcgaccaac
     6301 acgtcctgga gggcaaccac tcgctcggcg accgtcgcca ccgcgtcctt gcagaacagg
     6361 cggaacgccg tggccacctg gtcgttgcgg ctcctgccgg tgtggaggcg ggcgccggcg
     6421 tcgccggcca gctcggtgac ccgacgctcg atcgcggtgt ggatgtcctc gtcggtctcg
     6481 acgtaggtga actcgcccga ggccagctcg gcctcggtgg tatcgagggc tgcctcgacc
     6541 gctcccacct ccgacgcgct gaggatgccg gccacgccca gcccggccac gtgggcgcgg
     6601 cagccgcgga tgtcgaagcg ggcgaggcgc tggtcgaagg gcaggctgag cgtgtactcc
     6661 cacaactcgg cggcgggccc ttcggcgaat cggccgtgcc acaagggctg tgcggtgtgc
     6721 tcgttctcgt cagacatcgt tcttcaaact agtggtgggt aggttgggcc ggtgagactg
     6781 atcccctttg gaacgtcgtc gggtcgcccg acgccgtcgc ggaacgtcag cggtctcggg
     6841 gtcgacctcg gcagggagtg gatcctcgtc gactgcggcg aaggcaccca gcagcaggtc
     6901 atgcgcagtc cgctgaagtt gtcgaggctc tcggcgatcc tgatcaccca tctccacggc
     6961 gaccacgtgt tggggctccc cgggttgttg ggcacgatgg gcatggaggg ccgcaccgct
     7021 cccctgcagc tggtcggggt cgacgggatc ggtcgctggc tcgacgtgat gtgcgagctc
     7081 ccgatcctcg ggatcgactt cccgatcacg atcaccgagc tggtcgacga caccctcgcg
     7141 ggcgcggccg gcgagccgct ccacgtcgcc gacgtgggtg ggtttgccgt gagcgcgctg
     7201 gcactccgcc accgggtacc ggccttcggc taccggatcg tcgaaccgac gcgccgcggc
     7261 cacctcgatg tcgaggcggc ccgggcgctc ggcgtcaccg acggccccga cctggggctg
     7321 ctgcagcgag gcgagacggt gcccggccga acgggcccgg tgcatcccga ccaggtcgtc
     7381 ggccccgacc gggccggccg catcgtcacc gtgctcggcg acaccactcg ctgcgccgcg
     7441 tcggtctcgc tggcccgcga cgccgacctg gtggtccacg agtgcaccta cgcgaaccgc
     7501 gacgccgccc tcggggagca gtggaagcac tcgactcccg ccgacgcggc ctgggtggca
     7561 accagggccg gggcgagacg cctgctgatc aaccacttca gctcccgcta cgacgatccg
     7621 aacgagctga tcaccgaggc ccgggccgat ttccccgaca ccatcgccgc ccaggaactc
     7681 gagccggtcg acattccgct ccgctgaccg gttctgtccc gcgaggggat gcagattgca
     7741 cgcgttcgcg ggacagaacg ggcggcgtcg ggacagaacg aggggcgcgg gtcaggccga
     7801 gccggtcccc tggatggacg accacgtctt cacgctcagg ccccagagct gcacaaagcc
     7861 ctccgagtcg gcgtggcgga agcgatcggc ggcgtcgtag gtcgcgaggc cgtagtcgta
     7921 gagcgacttg ggtgcccggc gcccgaccac gaagcactgg ccgccggcgc cgtcgacacc
     7981 aggggtgagc gccaggcgaa cctcgccggt gacgtactgc tgggacgact cgatgaaggc
     8041 atcgagcgcc tgcttgaggg ggctgaacca caacccgtcg tagatcagct cggcgtagcg
     8101 gtgctcgagc ttcatcttct cgcggcccag gtcgcgttcg acggtgatcg actcgaggtc
     8161 ggcgtgggcc agcatcagcg ccaggcctcc gggcgcctcg taggtctcac ggctcttgat
     8221 gccgacacga cggttctcga ccatgtcgag gcggccccac ccgtaggcgc cgaccacgcc
     8281 gttgaggtgc accacgatct cgtgcagcgg catccgggcg ccgtcgaccg agaccggcac
     8341 accggcctcg aacccgatga cgacctcacg gcgctcggtc gccgtgggct tggtgagttc
     8401 ccacacaccc gacggcggct cggcccacgg gtcctccatc tcgccgcact cgatcgcacg
     8461 accccagagg ttgtcgtcga tggagtacat cttctccttg gtcgcccgga tcgggatgtt
     8521 gtgctgggcg gcgtaggcga tggagtcctc acgggtgaac ccccagttgc gcaccggggc
     8581 caccaactcg aggtcagggg cgagggccat ggtcgacacc tcgaagcgga cctggtcgtt
     8641 gcccttgccg gtgcacccgt gggcgatgcc gtcggcaccg aaccggcggg cgacctcgac
     8701 ctggtgcttg acgatcagcg ggcgggacag cgccgacacc aacgggtact tgccttcgta
     8761 gagggcgttc gccttgatga cgggagcgag gtagtcgcga gcgaactcct cgcgcaggtc
     8821 gagctcgatg tactcgctcg cgccggcgcc gagcgccttc tcacgattgc cgtcgagggt
     8881 gccctcctgg ccgacgttgg cggagacgca gatcacctcg caaccgagat tctcgatcat
     8941 ccaccgaacc gcgacagacg tatcgaggcc gccggagtac gcgagtacga ctttctttgc
     9001 catgtggctg ggatccttct tgatggtggg ggggggtcag aggttggcga ggtcggagat
     9061 cgcggcctcg accgcggcgc cggagccctg cgccgcgatc acgaggaggg tgtcgtcgcc
     9121 ggcgacggta ccgatgatgt cgggggtgcc cgaacggtcg atggccgacg ccacgacgtg
     9181 ggcgcatcct ggcggggtgc gcagcaccac gagatcgccg gatcggtcga gaccggcaac
     9241 ccagtcgccg agcacccgcc ggagatggtc gtcggtcgac acctggtcga acggcagctc
     9301 gggaatggcg tacgcggtct ccccgccggg cacccgcacc ttgacggccg acagctcctc
     9361 gaggtcgcgg ctgaccgtcg cctggttcac ctcgacaccg gcctccgcca gcagctcgac
     9421 gagctgggtc tggttggtga cctggttctc ctcgaggagt ttggtgatcc gatgctggcg
     9481 ggccgtcttc gagatgcggg gggtcacgat tcctccacga cggccggcgc cccggggtcg
     9541 tcggccgcca agaacagcca cgccaacagc cctcgcgccg agtggagccg gttgtgggcc
     9601 tggggccaga tccgcgcctg ggggccgtcg agcacgtcgt cggatgcctc gtcgccccgg
     9661 tgggcgggaa ggcagtggag gaagaccgcg tcgggggacg ccacgcccat gacgtcggca
     9721 tcgacctgga acccggcgaa gtcacggcgg cgctgcacca tctcggcctc ctgccccatc
     9781 gagtaccagg cgtcggtgta gacgacgtcg gcgtccttgg cggcctcgtg ggggtcaccg
     9841 gtggtgtcga ccgagacccc ggcggcagcg aggcgggcga cggcagcgtc gttgaacgcg
     9901 tatcccgccg gatgcgcgat ccgcagcgtg gcaccgcaca tggcgagacc gagggccagt
     9961 gagcgcgcca tgttgttggc gtcgccgacg tacgcgacgg tgacaccgtc gagccggccc
    10021 agctcctggc ggatcgtcag gagatcggcg agcacctgga tcgggtgggc atcgtcgctc
    10081 aggaggttga tgatcgggac ggacgacacc gctgccatgc gctcgagctt cgagtgctcg
    10141 aacacccggg ccgcgatcgc cgcgtggtac cccatgaggg tgcgggtgac gtcctcggtc
    10201 gactcacgca cgtcgaggcc gacctcctgg ggtcggacgt agaccggatg gcccccgagt
    10261 tcgatcgtgg cgatctccat cgagtggcgg gtacgggccg acggcttctc gaagagcagg
    10321 gccaccccgc gccggtgcaa cgccaggggg aggtcggtgg attcggcgag gtcgagcacg
    10381 tcgaccagct cagcgggagt gagatcgtcg atctcgagga agtgccgtgg catctccgca
    10441 ccctagcagc gagggcgcat gttcatgcac atacttgcat gaacatgtag ctcctggggg
    10501 ttctgtgctg cgaggagcga cgtcagcgtc gctcctcgca cgacagaacc ccgggggtca
    10561 cacaccggcg agctcgccaa ccgccccgat gatcgcggcg acggccagct tcacctcgtc
    10621 gtcggtgatc aacagactcg gagcgagccg cagcgccgtc ggactcaccg cgttcaccac
    10681 caccccgcgc gacaacagga acgccgccgc ctcgccggcg gcaacaccgt cgagctcgac
    10741 cgccaacagc agacccttgc cccggacccc ggcgatgccg ggacggccga cgagaccctc
    10801 ggcgatcgcg gcgccggcag cggccgagcg agcggggacg tcctcgcgca ccatgatctc
    10861 gagcaccttt cgagcggtcg ccgcggcgag cggctgaccc ccgtaggtcg tggcgtg
//
LOCUS       DAOSOA010000081        10832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6062_length_10832_cov_8.844576, whole genome shotgun sequence.
ACCESSION   DAOSOA010000081 DAOSOA010000000
VERSION     DAOSOA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6062_length_10832_cov_8.844576"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKY13_07075"
     CDS             complement(<1..445)
                     /locus_tag="PKY13_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79732.1"
                     /translation="MHYPNARFLLVNPRLMMAMTIHNAEMESLGIDMVADWMSDDYES
                     LDNVAPSATSNQALNRQIDEELRLLEPEGIAEGWRAFVPIDSDPEFDGYVLVDLRNAR
                     AGVGGDGRIVDFPTDVASATALMDAIPAECFLSGHEGGIRGCREAR"
     gene            complement(607..1218)
                     /locus_tag="PKY13_07080"
     CDS             complement(607..1218)
                     /locus_tag="PKY13_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79733.1"
                     /translation="MTSSPQSRQPRGVPSGGQFAAGTLSEAASDVHLRISAHQHEEWQ
                     MRESPSGGRYCAACGENENRPTLLDALKAVADETWLLQRKHELLAAKIAASIVRERFP
                     DARYLMLDHSGADGDEDTLVACGVILVDGTLADLDEEDLRDITGSGGGSEGEGLHFFT
                     SMLNGSDGCWTEFETTKHRLRTGPHLLDLELAEAWDFAEAVPS"
     gene            complement(1215..1685)
                     /locus_tag="PKY13_07085"
     CDS             complement(1215..1685)
                     /locus_tag="PKY13_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79734.1"
                     /translation="MTANRRHPAGTPAGGRFAPGMRNEAESVALDDQPTSVEAPDGTE
                     EWWSNGRLHRADGPAVVFSTGTQQWYSEGLKHRSDGPAVIFSDGAEAWYHYGRLHRGD
                     GPAYTSPDGTAEWWSDGRRHRDDGPAIILPDGAEMWFLHGVRMDPPNQPPTASV"
     gene            complement(1682..2233)
                     /locus_tag="PKY13_07090"
     CDS             complement(1682..2233)
                     /locus_tag="PKY13_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79735.1"
                     /translation="MTTNRRPPGTPTGGQFAPVDHPEASNVELDDATHRVAALLDDFG
                     TSVTEFSRLGRRTELLGAKLAATILRHHHPTARYLLVESNDQGGDDMVPVGLIDGDLN
                     DIDEEDLWALELPGCTDELFYFVGQMTDSDGPNYWTEFEAPEEERDRLGESHPEVIRR
                     HGPSVLDLDKALAWDPNGPSEQP"
     gene            complement(2230..3681)
                     /locus_tag="PKY13_07095"
     CDS             complement(2230..3681)
                     /locus_tag="PKY13_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79736.1"
                     /translation="MATNARHRAGAAGSVGGRFAPTSRRESDVAITDGTPCSSCGTST
                     KRSSGLCRRCDPKRTKPSGPVAGVEHVTRSGPILDRTVTVDIGDIVQCDYGFVPVYRG
                     GARVEGQVRGCRNAVRSPATTCHQHGGSTETSLGRTISKALAEAGRGECHPLAAEHWA
                     QTDERLAAAQGRLLGLLETDTTAIAQLYVEWRRQDADGVARFSPSNQMLVLAQHWANA
                     LDETEDADEADSDAAMQLALKRLKEPHLNVNAWAKKGRAPIDGEPGVAVVWWKPGSKP
                     KQKADETDEEYELRCARSRRGFHGAHLEFPLSSTDGDEYPLVEEPLAAARPTGFGDPD
                     AAISQMERLAADMGIKVVYTDHQDRGAIAYWLASESKINVWTGVGGGDARARAHSLAH
                     ELGHALLGHSTDAQAEERSAEKEVAAESFAALALAHHGIDSSEISAHYIEGWRRGAGI
                     DMKSAGLGPLRSAVVAFDGYVTATANENTGEQP"
     gene            complement(3721..8364)
                     /locus_tag="PKY13_07100"
     CDS             complement(3721..8364)
                     /locus_tag="PKY13_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOT79737.1"
                     /translation="MNATQSHLTALRAIARSIDGEPVTAEEMEAVAAWRGWGPAAKML
                     ENHSPHRSEIIATLDEEQLEAAKRATTTAFYTPPWLAASIWSGVRRSLGFTGGRVLEA
                     GCGSGLFFDDAVGELVGVEADPVTAEAARLRHPEATIVTSRFERFTAPAGSFDLVIGN
                     VPFGKDTPYDPIDNPAGLAIHNYFIAKALRLVRPGGVVAVVTSRFTLDAGTTKARDEI
                     ARWGSLLGVVRFPEGTFADMGTNVVADLLIFRRDDTDGSPRLADASWRASTKLAGRYD
                     LEIPRYMRNRDDTIPTMNTLFDVGSPTVGVVGELVVGSGMYGSGSVTWRLERGADVQR
                     EVTKTVARVFDAVAATHTFDHTAPLASFDPTAATPDTKVLPGTIRIAGSGFERFTAID
                     GWIGHDAGREAAELTALIELRDAVRTMLDDRDGRDNVVELHAAYTKRWGSLRRVGGTA
                     TAPRYPKLGGFRVDPGWPLVASLEIGLTAPAPEAEVVVTDLASAIAVSLDRTGGLDVD
                     LVAELLDWTADSVHEQLTDKRLAFADPLTGRWMMADEYLSGDVRTRHAQAVAAGAEPN
                     ISALAEVVPADATPEEVAARLGAAWIAPEIIAAFVNELVSPGCYGGGIKVEHAAEVGQ
                     WKVVPGPRKDYSTSLTAGTWGTPWMDTFDIVQGMLNMSALRVWESIFVNGAELKRIDN
                     AATEAVNEKAAEIAERFGEWVWEDPDRTETLLAEFNRRFRSHRGRNFDGSHQTFPGLN
                     PAFVPHDHQRDVVWRVVASGNTLIGHDVGAGKTASLIMSAMESRRLGLVNKPMIVVPN
                     HMLEQFTADFMSLYPKANVLAAGRHETSASERAEFVAAAAIGDWDAIVCTHNAFGRIG
                     LSDESMANYIRDEIAWFDAAIGENAEQRSVVKAIERRKRALEAKLERLVLGQQRDDTA
                     VSFEDLQVDALWVDEAHRYKNLAVNTRSDIATGNPSKTASDLDAKLAWIRSTYDPNRA
                     IVFATGTPISNTLAELWVVHHYVDPASLEEAGLAEFDTWLATFAEVVTGLEMDPSGTG
                     WRMKSRIARFSNAPEMVLMFSRFADIQTHEDLDLALPDCTRSIETVDCSPELAEAMEQ
                     FMERADQIRARMVKPDEDNFLKLSTDARHASLDPRLVGLDAPSDGGKIGRCVDRCTRN
                     RHGLQIVFCDLGTPGSTRFDMYAELRSQLVARGFESDRIRFVQDAKTDKAKESLFAAC
                     RNGDVDVVIGSTGTFGEGTNIQDRVTAIHHLDAPWRPSDIRQREGRGVRQGNPNHVRI
                     YRYVVTGSFDAFMWGTLERKARFIEQVYRSEARAIEDVDGDAVFTFAEVKAVASGNPA
                     LLRRAELENLIAKHRRSRTGHIRTNRHRRSMIKDLGVAMSNNVRLRDGLLILEHIDGA
                     LNSNPAFARRYSSTPFDAEEMAIEVLGGRDHQVMVNSRVTLTMRRRGARRIDAEFSFN
                     YDPLLTFEVSARDLRDEAAPTLHHLWATFLNIRPGRIAELDEEIATTEAEIASLTAQS
                     EAAWAGDGELAALEAELVAIDEELRQASVASVGSPTDRSVAEAADQSDEALVA"
     gene            complement(8370..8825)
                     /locus_tag="PKY13_07105"
     CDS             complement(8370..8825)
                     /locus_tag="PKY13_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79738.1"
                     /translation="MNEQAVDIWEAWATGRWAVIPTNCQTRPDGRAVMGAGLAKAAAA
                     RFSGIDRSYGRAIARGEVNLALPAHRLLLAPTKDHWSDPSSLDLVASSCHAVAAYAFR
                     RNVSLVVPKLGCGLGGLDWADVGPVVDDAFACVDYVIAAPVGTAHGPPT"
     gene            complement(8822..9157)
                     /locus_tag="PKY13_07110"
     CDS             complement(8822..9157)
                     /locus_tag="PKY13_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79739.1"
                     /translation="MNSWVVYLLGVATLPAAYVLIGAVDRSCVWIARRWVRVVCGHYE
                     FRDGYESKRIRVWRPTGTHRSGCVVTVRLTRTSRRGFDIGVGFRPYSPDELRARRIAA
                     RAAAADLAS"
     gene            complement(9154..9471)
                     /locus_tag="PKY13_07115"
     CDS             complement(9154..9471)
                     /locus_tag="PKY13_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79740.1"
                     /translation="MNLRIASTLRWESTHRSLNDVVTGRVIDANESEVTPLHVLTVLS
                     ADPDQIRASYTSWCDDNDVLALHDYLGRDGVTLVVPLFELTDAVCRDAIGRYMDDDIG
                     AAS"
     gene            complement(9468..9683)
                     /locus_tag="PKY13_07120"
     CDS             complement(9468..9683)
                     /locus_tag="PKY13_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79741.1"
                     /translation="MAPTDLLVDDGDINFDPYLDYSSDVSTAVRAGLDELTPERLGSS
                     SNRLNLDSLGPAREFSFGPERRRWRRR"
     gene            complement(9685..10452)
                     /locus_tag="PKY13_07125"
     CDS             complement(9685..10452)
                     /locus_tag="PKY13_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HOT79742.1"
                     /translation="MKSKPLPLAVVLIAALFVMQRCPAARDLATRIGHAGSVKEVTES
                     ISIPDISIPELSLPTLPTAPAQPSEPTPPLTATATATQLLEALPVAEPITAGYTRESF
                     KHWSDLDNDCFDARQQVLRDESTSQAQVDVGSYCTVVAGDWVSIYDGKEFTDPGELDV
                     DHFVPLSEAWKSGANTWDAGRREAYANDVTDPGHLVAVSATSNRSKGDKDPARWLPPV
                     QETRCWYVENWVSVKTRWGLSVDAAEKSAIARTLEAC"
     gene            complement(10449..10670)
                     /locus_tag="PKY13_07130"
     CDS             complement(10449..10670)
                     /locus_tag="PKY13_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79743.1"
                     /translation="MTSRRRDEPGWWQRDFDTASTATAIAVAALLVIAAVAFAFLPEP
                     NADVPPGWKNAYPHFIEVGTPALQPTFLP"
BASE COUNT         1602 a         3352 c         3964 g         1914 t
ORIGIN      
        1 gtcgagcctc ccggcatccc ctgatcccac cctcgtggcc gctcaagaag cactccgccg
       61 ggatcgcgtc catgagggcg gtcgccgacg ccacgtcggt cggaaagtcg acgattcgac
      121 catcccctcc gaccccggcg cgggcgttgc gcaggtcaac gagaacgtat ccgtcgaact
      181 cggggtcgct gtcgatcgga acgaaggccc gccaaccctc tgcgatgccc tccggttcga
      241 gcaaccgaag ttcctcatcg atctgacggt tgagtgcctg gttggatgtg gcgctgggag
      301 caacgttgtc gagggactcg tagtcatcgc tcatccagtc ggcgaccatg tcgatgccga
      361 gcgactccat ttcggcgttg tgaatcgtca tcgccatcat taggcgtggg ttgacgagaa
      421 ggaacctcgc gttcgggtag tgcaccgcta tcgccgaggc gatgcggcgg acagctgccc
      481 gctcgtcggc gagcagtcgg ccgaaggtgc cggcggacgg atcgaggtgc ggtgccaggt
      541 cacccccttc cgcgcgtact gcgtgagcga aacgtccgcc ggagggcgtg ccggagggtt
      601 ggcgcttcat gacggcaccg cctcggcgaa gtcccacgct tcggcgagct ccaggtcgag
      661 gaggtggggg cctgttcgca ggcggtgctt cgtcgtctcg aactccgtcc agcatccgtc
      721 gctgccgttc agcatggatg tgaagaagtg gaggccctcg ccttcacttc cgccgccaga
      781 gccggtgatg tcgcgaaggt cttcctcgtc gaggtcggcc agggtgccgt cgacgaggat
      841 cacgccgcac gccaccaacg tgtcctcgtc cccgtcggcg ccactgtggt cgagcatcag
      901 gtacctcgcg tcggggaacc gttcgcgcac gatcgaggcg gcgatcttgg cggccagcag
      961 ttcgtgtttg cgctgcagca gccaggtttc gtcggcgaca gccttgaggg cgtcgaggag
     1021 cgtcgggcgg ttctcgttct ctccgcaggc cgcgcagtag cgtccgcccg acgggctctc
     1081 tcgcatctgc cactcctcgt gctggtgggc cgagatgcgc agatggacgt cgctcgctgc
     1141 ttcgctgagc gttcctgcgg cgaactggcc gcccgacggg acacctcggg gctgacggga
     1201 ctgcggcgaa ctggtcatac cgatgctgtg ggtggctggt tgggcggatc catcctcact
     1261 ccgtgcagga accacatttc ggcgccgtcg gggaggatga tcgcggggcc gtcgtcgcgg
     1321 tggcgtcgac cgtccgacca ccattccgcg gtgccgtcgg ggctcgtgta agcggggccg
     1381 tctccgcgat gcaacctgcc gtagtggtac caggcctcgg cgccgtcgga gaagatgacc
     1441 gccggcccat cggaacggtg cttcagtcct tccgagtacc actgttgggt accggtcgag
     1501 aacacgacgg ctggcccatc ggcgcggtgc agtcgtccgt tcgaccacca ctcctcggtg
     1561 ccgtcgggtg cttcgacgct ggttggctgg tcgtcgagcg cgacgctctc cgcctcgttg
     1621 cgcatccccg gcgcgaagcg tccgccggct ggggtgccgg cgggatggcg ccggttggct
     1681 gtcatggctg ctcgctgggg ccgttcgggt cccacgcgag cgctttgtcc aggtcgagga
     1741 ccgatggacc gtgtcgacgg atcacctcgg gatgcgattc gccgagccgg tcccgctcct
     1801 cctcgggtgc ttcgaactct gtccagtagt tcgggccgtc gctgtcggtc atctggccga
     1861 cgaaatagaa gagctcgtcg gtgcagccgg gcagttcgag agcccagagg tcctcctcgt
     1921 cgatgtcgtt caggtcgccg tcgatcagcc cgaccggcac catgtcgtca ccgccctggt
     1981 cgttggactc gacgaggagg tatcgggcgg tcggatggtg gtgacggagg atcgtcgccg
     2041 cgagtttcgc gccgagcagt tcagtccgac gtccgaggcg gctgaactcg gtgacgctcg
     2101 tcccgaagtc gtcgagcagt gcggcgacgc ggtgggttgc gtcgtcgagt tcgacgttcg
     2161 aggcctcggg gtggtcgacg ggagcgaact ggccgccggt gggggttccg ggcggccgtc
     2221 ggttggtggt catggctgct ctccggtgtt ctcgttggcg gttgcggtca cgtagccgtc
     2281 gaacgcgacg acagcgctgc ggagcgggcc gaggccggcg gacttcatgt cgatgccggc
     2341 gccgcggcgc caaccttcga tgtagtgggc gctgatctcc gacgagtcga tgccgtggtg
     2401 ggcgagggcg agggcggcga acgattcggc cgccacctcc ttctccgcgg accgctcctc
     2461 ggcctgagcg tcggtcgaat gcccgagcag tgcgtggccg agctcgtggg cgagagaatg
     2521 ggcccgggcc cgggcgtcgc cgccaccgac cccggtccac acgttgatct tcgattccga
     2581 agccaaccag taggcgatcg cccctcggtc ctggtggtcg gtgtagacca ccttgatccc
     2641 catatcggcg gcaagacgct ccatctggga gatcgccgcg tcgggatcgc cgaatccggt
     2701 tgggcgggct gcggcgagag gctcctcgac gagggggtac tcgtcgccgt ccgtgctcga
     2761 cagcgggaac tcgaggtgtg caccgtggaa gccgcgtcgc gaacgcgcgc agcggagctc
     2821 gtactcctcg tcggtctcgt cggccttctg cttcggcttc gagccaggct tccaccacac
     2881 cacggcaaca cctggttccc cgtcgatcgg cgcccggccc ttcttcgccc acgcgttgac
     2941 gttgaggtgc ggctccttca acctcttgag ggcgagctgc atcgcggcgt cggagtcggc
     3001 ctcgtcagcg tcttcggtct cgtcgagggc gttcgcccag tgctgagcga gcacgagcat
     3061 ctggttcgac ggagagaagc gggcgacacc gtcggcatct tggcggcgcc actcgacgta
     3121 cagctgcgcg atggcagtgg tgtcggtctc gagcagtccg agaaggcgcc cctgagccgc
     3181 tgcgaggcgc tcgtcggtct gtgcccagtg ctcggcggca agcggatggc actcgccgcg
     3241 ccccgcctca gcgagcgcct tggagatcgt gcgtccaagg ctcgtctcgg tgctcccgcc
     3301 gtgttggtgg caggtggtcg ccggggagcg gacggcgttt cggcacccgc gcacctggcc
     3361 ttcgactcga gcgcctccgc ggtagacggg tacgaacccg tagtcgcact ggacgatgtc
     3421 accgatgtcg acggtgacgg ttcggtcgag gatcggcccg gatcgggtca cgtgttcgac
     3481 gccggcgacg ggccccgacg gcttggtgcg cttggggtcg cagcggcggc acagcccgga
     3541 gctgcgtttg gtcgacgtgc cacaggacga gcagggggtg ccgtctgtga tcgcgacgtc
     3601 ggattcgcgt cggcttgttg gggcgaagcg accgccgacg gacccggcgg ccccggcgcg
     3661 atgtcgtgcg ttggtggcca tggaccgcat gtgggttgcc gaagcggccg ctcctccggg
     3721 ttaggcgacg agcgcctcgt cgctctggtc ggctgcctcg gcgaccgacc tgtcggtcgg
     3781 ggatccgacg gaggcgacgc tggcctgacg cagctcctcg tcgatcgcga ccagttccgc
     3841 ttcgagggcg gcgagctcgc cgtcgcccgc ccacgccgct tcggactgcg ctgtgagaga
     3901 ggcgatctcg gcttcggtgg tggcgatctc ctcgtcgagt tcggcgatgc gtccggggcg
     3961 gatgttcagg aacgtcgccc acaggtggtg aagcgtcggc gccgcttcgt cgcggagatc
     4021 tctggcgctg acctcgaagg tgaggagcgg gtcgtagttg aacgagaact cggcgtcgat
     4081 gcggcgggcg ccgcggcgcc gcatcgtcaa ggtcacgcgg gagttgacca tgacctggtg
     4141 gtctcggccg ccgagcacct cgatcgccat ctcctcggcg tcgaacgggg tcgagctgta
     4201 acggcgagcg aaggcagggt tggagttgag tgcgccgtcg atgtgttcga ggatcagcag
     4261 cccgtcacgg agccgcacgt tgttggacat agcgacgccg agatccttga tcatggagcg
     4321 ccggtgccgg ttggtgcgaa tgtggccggt gcggctgcga cgatgcttgg cgatcaggtt
     4381 ctcgagctcg gcgcgtcgca gcagggctgg gttgccggag gcgacggcct tcacctcggc
     4441 gaacgtgaac actgcatcgc cgtcgacatc ttcgatggcc cgagcttcgc tgcggtacac
     4501 ctgctcgatg aagcgggctt tgcgttcgag ggttccccac atgaaggcgt cgaaggaacc
     4561 ggtgacgacg tagcggtaga tccggacatg gttcgggttc ccctgccgga cgccgcgacc
     4621 ttcgcgctgg cggatgtcgg agggccgcca gggggcgtcg aggtggtgaa tggcggtcac
     4681 ccgatcttga atgttggtgc cttcgccgaa ggtaccggtc gagccgatca cgacatcgac
     4741 gtcgccgttg cggcatgcgg cgaacaggga ctctttcgcc ttgtcggtct tggcgtcctg
     4801 cacgaaccgg attcggtcgg actcgaatcc gcgtgcgacg agctgggacc gcagctcggc
     4861 gtacatgtcg aatcgggtgg agccgggggt gccgaggtcg cagaacacga tctgcaggcc
     4921 gtggcggttg cgtgtgcacc ggtcgacgca tcggccgatc ttgccgccgt cgctgggtgc
     4981 gtcgagcccg acgaggcggg ggtcgagtga agcgtgccgg gcgtcggtgg agagcttcaa
     5041 gaagttgtct tcgtcgggct tcaccattcg ggcccggatc tggtcggcgc gctccatgaa
     5101 ctgctccatg gcttcggcga gctcgggcga gcagtcgacc gtttcgatgc tgcgcgtgca
     5161 gtcgggcaac gccaggtcga gatcctcgtg ggtctggatg tcggcgaagc ggctgaacat
     5221 gaggaccatc tcgggggcgt tcgagaagcg tgcgatgcgc gacttcatcc gccacccggt
     5281 gcctgacgga tccatttcga gtccagtgac gacctcggcg aacgtggcaa gccaggtgtc
     5341 gaactcggcg agcccggcct cttcgagcga cgccgggtcg acgtagtggt gcacgaccca
     5401 cagctcggcg agcgtgttgc tgatcggggt gccggtggcg aacacgatgg cccggttcgg
     5461 gtcgtaggtg gagcggatcc aggcgagctt ggcgtcgaga tccgacgcgg tcttggacgg
     5521 gttgccggtg gcgatgtcgg atcgcgtgtt gaccgcaagg ttcttgtacc ggtgagcttc
     5581 gtcgacccac agagcgtcaa cctgcaggtc ctcgaaggac acggcggtgt cgtcgcgctg
     5641 ctggccgagc acgaggcgtt ccaacttcgc ttcgagggcg cgtttgcggc gttcgattgc
     5701 cttcacgacc gatcgctgct cggcgttctc accgatcgcc gcatcgaacc atgcgatctc
     5761 gtcgcggatg tagttggcca tcgactcgtc agagagtccg atccgaccga acgcgttgtg
     5821 cgtacacacg atcgcgtccc aatcaccgat cgcggcggca gcgacgaact cggctcgctc
     5881 ggaggccgag gtctcatggc ggcccgcggc gagcacgttc gccttcgggt acagcgacat
     5941 gaagtccgcg gtgaactgct cgagcatgtg gttcgggacg acgatcatcg gcttgttgac
     6001 cagcccgagc cgacgcgact ccatcgccga catgatcagc gacgcggtct tgccggcgcc
     6061 cacgtcgtgt ccgatcaggg tgttcccgga tgcgacgaca cgccacacga catcgcgctg
     6121 atgatcgtgg ggcacgaacg ccgggttgag gccggggaac gtctggtggg agccgtcgaa
     6181 gttgcggccg cggtgggatc ggaaccggcg gttgaactcg gcgagcaggg tttcggtgcg
     6241 gtcggggtct tcccacaccc actcgccgaa ccgctccgcg atctcggcgg ccttctcgtt
     6301 gaccgcttcg gtcgcagcgt tgtcgatccg cttcaactcc gccccgttga cgaagatcga
     6361 ctcccacacg cggagcgccg acatgttgag catgccctgg acgatgtcga acgtgtccat
     6421 ccagggggtt ccccaggtgc cggccgtgag cgacgtcgag tagtccttcc gagggccggg
     6481 gacgaccttc cactggccga cctcggctgc gtgttcgact ttgatgccac cgccatagca
     6541 gccgggggag acgagctcgt tgacgaatgc ggcgatgatc tcgggagcga tccacgctgc
     6601 gccgagccgg gcggcaacct cttcgggggt cgcatcggcg ggcacgacct cggcgagagc
     6661 ggagatgttg ggctccgcgc ccgctgcgac cgcttgggcg tgacgggtgc ggacgtcgcc
     6721 ggacaggtac tcgtcggcca tcatccagcg gccggtgagc gggtcggcga acgcaagacg
     6781 cttgtccgtc aactgctcgt gaaccgagtc ggcggtccag tcgaggagtt cggcgaccag
     6841 gtcgacatcg agaccaccgg tgcggtcgag cgagactgca atcgcgctcg ccaggtcggt
     6901 gacgacgacc tcggcctcgg gcgctggtgc ggtcaggccg atctcgaggc tggcgacaag
     6961 tggccaaccc gggtcgacgc ggaacccgcc gagcttcggg tagcggggtg cggtggctgt
     7021 cccgccaact cgtcggaggg aaccccaccg cttcgtgtag gcggcgtgca gttcgacgac
     7081 gttgtcgcgg ccgtcccggt catcgagcat cgttcggacg gcgtcgcgca gttcgatgag
     7141 ggcggtcagt tcagccgcct ctcggccagc gtcatgtccg atccacccgt cgattgcggt
     7201 gaaccgttcg aagccgctgc ctgcgatccg gatggtgccc ggcagaacct tcgtgtcggg
     7261 agtggcggcg gtcgggtcga acgacgcgag cggggctgtg tggtcgaacg tgtgggtggc
     7321 agcgacggcg tcgaagactc gtgcgaccgt cttggtcact tcgcgttgga cgtcggcgcc
     7381 acgttcgagg cgccacgtca ccgaacccga gccgtacatg ccggacccga cgaccagttc
     7441 gccgacgacg ccgacggtag gggagccgac atcgaacaac gtgttcatcg tcgggatggt
     7501 gtcatcgcgg ttgcgcatgt agcgggggat ctcgaggtcg tagcggccgg cgagcttggt
     7561 ggatgcacgc cacgaggcgt cggcgaggcg aggggagcca tcggtgtcgt cacgacggaa
     7621 gatcaggagg tcggcgacga cgttggtccc catgtcggcg aacgttcctt cggggaagcg
     7681 gacgacaccg aggagcgagc cccagcgggc gatctcgtcg cgggctttgg tggtgccggc
     7741 gtcgagcgtg aagcgtgacg tgacgactgc gacgacgccg ccggggcgga cgagacggag
     7801 tgccttggcg atgaagtagt tgtgaatggc aaggccagcc ggattgtcga tcgggtcgta
     7861 gggggtgtcc ttgccgaatg ggacgttgcc gatgacgagg tcgaacgatc cggcaggagc
     7921 ggtgaacctc tcgaatcggc tggtgacgat cgtggcttcg gggtgtcgga gccgtgccgc
     7981 ttcggcggtg actgggtcgg cttcgacgcc gacgagctca ccgaccgcgt cgtcgaagaa
     8041 cagtccggac ccgcaaccgg cttcgaggac ccgaccgcca gtgaacccga gcgaacgacg
     8101 gacgcccgac cagatcgagg ccgccagcca tgggggcgtg tagaacgcgg tggttgtagc
     8161 ccgcttcgct gcctcgagct gttcctcgtc gagcgtcgcg atgatctcgg agcggtgcgg
     8221 cgagtggttc tcgagcatct tcgccgccgg cccccacccg cgccacgcgg cgaccgcttc
     8281 catctcttcc gcggtgacgg gctctccgtc gatgctccgg gcgatggcgc gcaaggcggt
     8341 gaggtgggac tgcgtggcgt tcatgggttc tatgtgggcg ggccgtgagc ggtccccacc
     8401 ggtgcggcga tgacgtagtc gacgcatgcg aatgcgtcgt cgacgactgg gccgacgtcg
     8461 gcccagtcga gccctccgag cccgcacccg agcttcggca cgacgaggct gacgttgcgc
     8521 ctgaacgcgt aggcggcgac tgcgtggcag ctcgatgcga caaggtcgag gctcgaaggg
     8581 tcggaccaat ggtccttggt cggggcgagg aggagtcgat gtgccggcaa tgccaggttg
     8641 acttctcccc gagcgatggc gcggccgtag gagcggtcga tgccgctgaa ccgtgcggcg
     8701 gctgccttgg cgaggccggc gcccatgacg gcccgcccgt cggggcgggt ctggcagttc
     8761 gttgggatga cggcccaccg gccggtcgcc cacgcctccc agatgtcgac cgcttgttcg
     8821 ttcacgacgc caggtctgcg gcggcggctc gagcggcgat gcggcgcgcc cgaagttcgt
     8881 cgggcgagta gggccggaag ccgaccccga tgtcgaagcc gcgtctggac gttctcgtca
     8941 atcggacggt gacgacgcat ccgctccggt gggtgccggt cggacgccac actctgatgc
     9001 gcttcgactc gtatccgtcg cgaaactcgt agtgcccgca gacgactcgc acccatcgcc
     9061 gtgcaatcca gacacaggaa cgatcgacgg cgccgatgag aacgtaggcg gcgggcaagg
     9121 ttgcgacgcc gagcaggtag acgacccaac tgttcacgac gcagccccga tgtcgtcgtc
     9181 catgtaccgg ccgattgcgt ccctgcacac cgcgtcggtg agttcgaaca gcgggacgac
     9241 cagcgttacc ccgtcgcggc cgaggtagtc gtggagtgcg aggacgtcgt tgtcgtcgca
     9301 ccacgaggtg taggaggccc ggatctggtc agggtcggcg gacaggacgg tcaggacatg
     9361 gagaggggtg acctcggatt cgttggcatc tatgacgcgg ccggtgacga cgtcgttgag
     9421 gctgcggtgg gtcgattccc agcgcagtgt tgaagcgata cggagattca tcgccgccgc
     9481 cagcgccgcc gctcggggcc gaacgagaac tcccgggcag ggccgagact gtcgaggttg
     9541 agacggttcg aggacgaccc gaggcgttcc ggagtcagtt cgtcgaggcc ggccctcacc
     9601 gccgtcgaca cgtcggagga gtagtcgagg tacgggtcga agttgatgtc gccgtcatcg
     9661 acgaggaggt cggtgggggc cacgtcagca tgcctccagc gttcgggcga tggcggactt
     9721 ctcggcagcg tcgacgctga ggccccatcg ggtcttgacg gagacccagt tctccacgta
     9781 ccagcatctc gtctcctgca ccggtggcag ccaccgtgcc gggtccttgt cgcccttgga
     9841 acggttggag gtagcgctga cggcgacgag atggcctggg tcggtgacat cgttggcgta
     9901 cgcctcgcgt cggccggcat cccaggtgtt ggctccggac ttccaggcct ccgacagggg
     9961 tacgaagtgg tcgacgtcga gctcgccggg gtcggtgaac tccttgccgt cgtagatcga
    10021 cacccagtcg ccggccacga ccgtgcagta cgagccgacg tcgacctggg cctgggaggt
    10081 ggactcgtcg cgaaggacct gctggcgggc gtcgaagcag tcgttgtcga gatcggacca
    10141 gtgcttgaag gactcacggg tgtagccggc ggtgatgggt tctgcgaccg ggagcgcctc
    10201 gagcaactgg gtcgcggtcg cggtggcggt gaggggcggc gtgggctcgc tgggttgtgc
    10261 cggtgctgtc gggagggtgg gcaggctcag ctcggggatc gagatgtcgg gaatggaaat
    10321 cgattcggtg acctctttga ccgagccggc atggccgatc cgggtggcga gatcgcgtgc
    10381 ggcggggcat cgctgcatca cgaagagcgc tgcgatcagc acgacggcga gagggagtgg
    10441 cttcgacttc atggaaggaa tgtgggttgg agcgcagggg tccccacctc gatgaagtgg
    10501 gggtaggcgt tcttccagcc cggcggcacg tcagcgttgg gttcagggag gaatgcgaag
    10561 gcgacggccg cgatgaccag gagggcggcg acagcgattg cggtggctgt gctggcggta
    10621 tcgaagtcgc gctgccacca gcccggttcg tcgcgacggc gcgaggtcat aggccgcccc
    10681 agtcgctcgg gggccacacg atcgcctcgg ctcgaccgtt gatggcggtg acggggatcg
    10741 ttccgaaggt ccggctgtcc tgagaacgcg ggcggttgtc cccgaggacg aacactcgac
    10801 ctggggcaac gatggcgtcc atcgccaacg tg
//
LOCUS       DAOSOA010000082        10806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6080_length_10806_cov_6.400335, whole genome shotgun sequence.
ACCESSION   DAOSOA010000082 DAOSOA010000000
VERSION     DAOSOA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6080_length_10806_cov_6.400335"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..806)
                     /locus_tag="PKY13_07135"
     CDS             complement(12..806)
                     /locus_tag="PKY13_07135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT79744.1"
                     /translation="MSRLMQHPWRSAVVTGASSGIGEAFARSLADAGVDVVLVARRVD
                     RLDALADGLRAKDVIVEVLAADLASVDGRALVADRLSDGDRPIDLLVNCAGLGAATKF
                     HEGDPDRYRQIIDVNVDAVVELSHAVLPSMVRTGRGWILNVSSLGGLAPGPGFAVYSA
                     TKAFVCSFSESLHEEYRGTGVVVTAVCPGATRTEFGEGSGADSDDLPSLLWQEPEEVV
                     AEGLAAVAAGHALRVTGWPNRLAAGATALLPRIARRRIAGKVTDTL"
     gene            864..2678
                     /locus_tag="PKY13_07140"
     CDS             864..2678
                     /locus_tag="PKY13_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOT79745.1"
                     /translation="MPSPRIEDYAFLSDTRSAAMVSRSGSVDWLTFPRFDSPAAMAAL
                     VGTEENGHFSISPVGEFRTERRYLPGTLVLETTHTTPNGTAVVTDALAMPHHHRSTPR
                     LLRKVVVTSGRVAMRMELRIRFGYGRIVPWVRRPDHHLLAIGGPDALVLRTSVDVHGE
                     GLATVADFEVGEGTGAWFDLAWFESQNATPHPIAVNDSIDAAFDDTITRWRGWSDGIT
                     YDRGWSEEVKASAVVLKGLTYSPTGAIVAAPTTSLPETPGGARNWDYRYCWLRDATFT
                     LYAFVQAGLRAEATHWRDWLLRACAGDPDKLQIMYGLGGERWLPEFELDWLEGFSGAR
                     PVRVGNGAADQLQLDVFGEVMDSMYQSRVAGIPADDFSDALLAALLEHLETIWQQADE
                     GIWEVRGDRQHFTHSKVMSWVAFDRAAKSARRWGGDEGRWRQLADEVRREVCERAVDA
                     TGVFRQAYGSTALDASVLRIPLVGFLPPSDPRVLATVEAIERDLAVDGLLQRYNTDDT
                     DDGLEGDEGAFLLCSFWLADCLDLVGRRDDAVALYERLLALRNDVGLLAEEYGTTFAT
                     QLGNCPQAFSHVAIINTATNLCDDTPSGSGTSRVRLAD"
     gene            complement(2675..3160)
                     /locus_tag="PKY13_07145"
     CDS             complement(2675..3160)
                     /locus_tag="PKY13_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79746.1"
                     /translation="MPTPNPSRQPDPAVPRWSWIAAVGLVAASPGATWFWAGRLSEPE
                     LNPAVADYAWRPWRSLVEHESLVGAVSTGVALAAALALIVMRRRVGGWAMAPVLATTL
                     ALAVLGAWAGLGYRVITAAVVGANIGAGLVILATPALVFVELVAVVVWRSRRRQHQRV
                     G"
     gene            complement(3227..4129)
                     /gene="corA"
                     /locus_tag="PKY13_07150"
     CDS             complement(3227..4129)
                     /gene="corA"
                     /locus_tag="PKY13_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOT79747.1"
                     /translation="MAQMRDEHAFAWIGLVEPTADELEAVAMTFDLHPLAVEDALHAH
                     QRPKLERYDDHSFLVFKTARYVDHDEVIALGEVLLFVGEGFIISVRHGDAGNMGDIRR
                     RLEAEPERLALGPGVVVYELADRIADEFIGALGSVESDIDSIQSDVFAGPKASHASRI
                     FNLKREVLEFRQAANPLIDPLDELMRILPPGFVEELQPYFRDVHDHVLRATQRLAAID
                     DLLSSALDANVAQVSMRQNEDMRKISAWAAIVSVPTAIAGVYGMNFRHMPELEWRYGY
                     LIVIAAIGLICFGLYRNFKRREWL"
     gene            complement(4276..5472)
                     /locus_tag="PKY13_07155"
     CDS             complement(4276..5472)
                     /locus_tag="PKY13_07155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT79748.1"
                     /translation="MADAVIIDAVRTPLGRGKPGGILSSTHPVELLAHTLRAVVERNG
                     LDPEVIDDVIAGNVTQVSEQAFNIGRRAVLAAGYPERVPATTIDRQCGSSQQAIHFAA
                     QAIMSGTQDVVVAAGVESMSRVPMGSSALGAADLDGPSFDERYPDGLVSQGIASELIA
                     ARWGLSRAEVDEFALRSQVNAAAARDRGRFDHEIVPLEVVGPDGDLQLVTSDEGIRES
                     TIDGLAKLRPAFGADWASERFPQIQWVTTAANSSQLTDGAAAVLVTSDAKAKELGLTP
                     RARIHSLAVEGDDPLYMLTAVIPATKKLLARAGLTIDDIDLFEVNEAFASVVLAWQKE
                     LGADPDKVNVNGGAIALGHPLGASGARLAGTLLNALEQRDGRFGLQVMCEAGGLSNAM
                     IIERLD"
     gene            5584..6063
                     /locus_tag="PKY13_07160"
     CDS             5584..6063
                     /locus_tag="PKY13_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT79749.1"
                     /translation="MKRTSVKDMNCSVAQCLEVIGDWWTPLIVRDLLFGVGRFDEIQE
                     RLGISRNVLTQRLELLVENGVVERQPYQDNPPRFDYVLTRKGRDLWFVIQAMKQWGDT
                     WAAPDGPPIELLHTTCDHVVTVVPSCSACGERLSVGDVRAVAGPGATDRSPLPTHRR"
     gene            complement(6088..6975)
                     /locus_tag="PKY13_07165"
     CDS             complement(6088..6975)
                     /locus_tag="PKY13_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOT79750.1"
                     /translation="MDLRQLSYVVAVVDHGGFTRAAEALHVAQPSLSQAIKALETEVG
                     VALFDRVGRQVRLTAAGRALLPAARQALHDVEIAREAVDAVRGLGRGRLDLVSLPTLG
                     VSPVADFIGAFRTRNPDVTVRLVEPDEVGAVAANVAGGESEIGFTELPIADERLVTIE
                     LSGQEYVAVHHRSVERGSLVSVPQLAELPLVTTTPGTSTRRLIDEAFRQSGVAPRIAI
                     ETDLREVITAIVEAGGGYSILPRTVAERMAARGSGDVRVAEIIPTISRRIGVVHRQGT
                     LSPAGRAFLDLVLRSLPPD"
     gene            7046..7804
                     /locus_tag="PKY13_07170"
     CDS             7046..7804
                     /locus_tag="PKY13_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT79751.1"
                     /translation="MASVPLICTAGLAVGVLYGLFGVGSAFATPLLALLGVPGMAAVA
                     GPLPALLPGSMTGAWSYSRDGKVDWWVAKRTVAGAFPAAIVGAIASQWVSGPVLVAIS
                     GAVLLLIGLRVLRPVDPDATRPVWAHDHAGLLGASAIVIGFSSGLLANGGGFLLVPLF
                     LLAVGLDMNRATGTSLVVASALTIPTLITHAAIGDIDWAVSLVFALGLIPGAWVGALA
                     SKRFATDKLRGAFGFLLVGFAVWFLVREVPALLA"
     gene            complement(7857..9122)
                     /locus_tag="PKY13_07175"
     CDS             complement(7857..9122)
                     /locus_tag="PKY13_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT79752.1"
                     /translation="MAATTTPSASSLADIDLLSRDVFADRVPHEWFERLRAQAPIHHH
                     PEPDGPGFWAFTSHRDVGALNRDWHTFSSEHALGGVVQLEEPTPEQAVRRAEIGDAGR
                     LMLEMDPPDHTRFRKLLNRGFTPKVIRSLEDHLRLMSERIVDRAMAADGGRCDFVVDI
                     AAELPLEAIAEFLGVPFEDRHKIFDWSNRLIGTDDPEYAVSEDLLNEALVSMFMYAND
                     LGTDRRTDPRDDIVSKLIHEVVDGDQLTEMDFNQFFLLLSVAGNETTRNALSHGMTAF
                     LANPDQYRLLLDDPSLIDSAVEEILRWASPVMFFRRNVTTDTEYDGHQLKAGDKVSLW
                     YISANRDESVFDDPYRFDITRDPNPHIAFGGGGPHFCLGANLARMEIRLLFQALVDRA
                     PNIEQLGEPERLRSNFINGIKHLPVRLSS"
     gene            complement(9269..10123)
                     /locus_tag="PKY13_07180"
     CDS             complement(9269..10123)
                     /locus_tag="PKY13_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOT79753.1"
                     /translation="MELLDSRAATVGGTPVTRLLPKRAHRTVGPWCFADHFGPVDLGP
                     APTRDGAMAVGPHPHIGLQTVTWLFAGEVLHTDSVGSEQLIRPGQLNLMSAGRGISHA
                     EESPDDAAGEMHGLQLWVAQPDATRWSEPEFQHLAELPVVELGEATATVLVGELAEAR
                     SSARADWPMIGTDVVVRGEAEVPLDPTFEHAVLVMSGSPAVDGQHVPVGTTAYLAPGR
                     PSVTLGGPARVVILGGAPFESTIAMSWNFVARTTEEIDSAYADWAAAGARFGEVRSEL
                     ARIPAPAH"
     gene            complement(10123..>10806)
                     /locus_tag="PKY13_07185"
     CDS             complement(10123..>10806)
                     /locus_tag="PKY13_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HOT79754.1"
                     /translation="HQDSHGGGGLITNGATQWMTAGGGILHIETPPEELVVSGGLFHG
                     VQLWVNLPAKDKMIDPAYQNLEADLVTLVSSPDGGALVRLIAGDVDGHTGPGGTHTPI
                     VMAHVTVAPGAQVVVPWNPQFNALAYVLGGGGTVGAERRPAVTGQLAVFGAGDTITLG
                     GGAGERAGEPMEVLLLGGQPIREPIAQYGPFVMNTRAELAQAIDDFNNGLLGLVPTDG
                     LHPYRGPLG"
BASE COUNT         1624 a         3764 c         3769 g         1649 t
ORIGIN      
        1 ggcgtctccg atcagagtgt gtcggtgacc ttgccggcga tgcgacgccg ggcgatccgg
       61 ggcagcagcg cggtcgcccc ggccgcgagc cggttgggcc aacccgtgac ccggagcgcg
      121 tgcccggcgg cgaccgccgc gagcccctcg gcgacgacct cctcgggctc ctgccagagc
      181 aacgacggca ggtcatcgct gtcggcgccg gagccctcac cgaactcggt tcgcgtcgcg
      241 ccggggcaca cggctgtcac gacgacgccg gtgccgcggt actcctcgtg cagcgattca
      301 ctgaagctgc agacgaacgc cttcgtcgcc gagtagacgg cgaaccccgg gcccggcgcg
      361 agccccccga gcgatgacac gttgagaatc cagccacggc cggtccgcac catggacggc
      421 aggaccgcgt ggctgagctc gaccaccgcg tcgacgttca cgtcgatgat ctggcggtac
      481 cggtccgggt cgccctcgtg gaacttggtc gccgcgccga ggcccgcaca gttgacgagg
      541 aggtcgatcg gccggtcgcc gtcggacagc cggtccgcga cgagggcccg gccgtcgacc
      601 gacgcaaggt ccgccgcgag gacctccacg atgacgtcct tcgcccgcag gccgtcggcc
      661 agggcgtcga gccgatccac gcgccgcgcc acgagtacga cgtcgacgcc ggcgtcggcc
      721 aacgaccggg cgaacgcctc gccgatcccg ctcgatgccc cggtcacgac ggcggaccgc
      781 cacggatgct gcatcagtct gctcatcgcc tcaacctaga gcgacggtcc gtcgtgacac
      841 ggcgcgggta ccgtgagccg catatgccat cgccgaggat cgaggactac gcgttcctct
      901 ccgacacgcg ttcggcggcg atggtgtcgc gatcgggcag cgtcgactgg ctgaccttcc
      961 cccgcttcga ctccccggcc gcgatggcag cgctcgtcgg caccgaggag aacggtcact
     1021 tctcgatcag ccccgtcggc gagttccgca ccgaacggcg gtaccttccc ggaacgctcg
     1081 tcctggagac gacgcacaca acgccgaacg ggaccgccgt cgtgaccgac gcgctggcca
     1141 tgccccacca tcaccggtcg accccgcgcc tgctccggaa ggtcgtggtc accagcggcc
     1201 gggtggcgat gcggatggaa ctccgcatcc gcttcgggta cggccgcatc gtgccgtggg
     1261 tgcgccggcc cgaccaccac ctgcttgcca tcgggggacc cgacgcgctg gtgttgcgta
     1321 ccagcgtcga cgtccacggt gaagggctgg cgaccgtcgc cgacttcgag gtcggtgagg
     1381 gcaccggtgc ctggttcgac ctcgcgtggt tcgagtcgca gaacgcgacg ccgcatccga
     1441 tcgcagtcaa cgactccatc gacgctgcgt tcgacgacac catcacccgt tggcgcggct
     1501 ggtccgacgg catcacgtac gaccggggct ggtccgagga ggtcaaggcg tcggccgtcg
     1561 tcctcaaggg cctcacctac tcaccgaccg gcgcgatcgt cgcggcgccg accacctcgc
     1621 tgcccgagac cccgggcggg gcccggaact gggactaccg ctactgctgg ctgcgagatg
     1681 cgacgttcac gttgtatgcg ttcgtccagg ccgggctccg cgccgaggcc acacactggc
     1741 gcgactggtt gctgcgagcc tgcgccgggg accccgacaa gctgcagatc atgtacggcc
     1801 tcggcggtga gcggtggctg cccgagttcg agctcgactg gctcgagggg ttcagtgggg
     1861 cgcgtccggt gcgcgtcggc aacggggccg ccgaccagct gcagctcgac gtgttcggcg
     1921 aggtcatgga ctcgatgtat cagtcccgcg tcgccggcat cccggccgac gatttcagcg
     1981 acgccctcct cgcagcgctt ctcgaacacc tcgagacgat ctggcagcag gcggacgaag
     2041 gcatctggga ggtccgcggc gaccgtcagc acttcaccca ctcgaaggtg atgtcgtggg
     2101 tcgcgttcga ccgggccgcg aagtcggcgc ggcggtgggg cggcgacgaa ggccgctggc
     2161 gtcagctcgc cgacgaggtg cgtcgtgaag tgtgcgagcg ggccgtcgac gcgaccggcg
     2221 tcttccgtca ggcctacggc tcgacggcac tcgacgcatc agtgctgcgc atccctctcg
     2281 tcgggttcct gcccccgtcg gatccccgcg tgctcgctac tgtcgaggcg atcgaacgcg
     2341 atctcgcggt cgacggcctg ctgcagcggt acaacaccga cgacaccgac gacgggctcg
     2401 agggagacga gggagcgttc ctgctctgct cgttctggct ggccgactgc ctcgacctgg
     2461 tcgggcgccg cgacgacgcg gtcgcgctct acgaacggct cctcgccctc cgcaacgacg
     2521 tcggcctgct cgccgaggag tacggcacca cgttcgccac acagctgggc aactgcccgc
     2581 aggccttctc ccacgtggcg atcatcaaca ccgccaccaa cctgtgcgac gacacccctt
     2641 ccggctcggg cacgtcgcgg gtccggttgg ccgactaacc gacccgttgg tgttgtcggc
     2701 gacgagaccg ccagacgacg acggccacca gttccacgaa gaccagcgcc ggcgtggcga
     2761 ggatcaccag ccccgcgccg atgttggcac cgacgaccgc cgccgtgatg actcggtacc
     2821 ccagtccggc ccaggccccg agcacggcga gcgccagcgt cgtcgccagc accggggcca
     2881 ttgcccatcc gccgacgcgc cgccgcatca cgatcagggc cagggcggcg gcaagagcca
     2941 cccccgtcga gacggcgccg acgagggact cgtgttcgac cagcgagcgc cagggtcgcc
     3001 aggcgtagtc ggcgacggcg gggttcagct ccggttcgga cagtcggccg gcccagaacc
     3061 acgtggcgcc ggggctggcc gcgaccagtc ccaccgcagc gatccacgac caccgaggca
     3121 cggccggatc gggttgacgg gacgggttcg gcgttggcat ggatccagca tcgctccccg
     3181 ggttcgtggt ggccgtaggg gctcccccct catttcgcct ggggcctcag agccactcgc
     3241 ggcgcttgaa gttgcggtac aacccgaagc agatgagccc gatcgccgcg atcacgatga
     3301 ggtagccgta gcgccactcg agctcgggca tgtggcggaa gttcatgccg tacacaccgg
     3361 cgatcgcggt gggcaccgac acgatcgcgg cccacgccga gatcttgcgc atgtcctcgt
     3421 tctggcgcat cgacacctgg gcgacgttgg cgtcgagcgc gctcgacagg aggtcgtcga
     3481 tggcggcgag gcgctgcgtg gcccggagga cgtggtcgtg cacgtcgcgg aagtacggct
     3541 gcagctcctc gacgaacccg ggcgggagga tgcgcatgag ttcgtcgagc ggatcgatca
     3601 gcgggttggc ggcctggcgg aactccagca cctcgcgctt gaggttgaag atgcggctgg
     3661 cgtgggacgc cttcggcccg gcgaacacgt cggactggat cgagtcgatg tcgctctcga
     3721 ccgacccgag cgcgccgatg aactcgtcgg cgatccggtc ggccagctcg tagaccacca
     3781 cgccgggccc gagcgccagc cgttcgggct cggcctcgag ccgacggcgg atgtcaccca
     3841 tgttgccggc gtcgccgtga cggacgctga tgatgaaccc ctcaccgacg aacaggagca
     3901 cctcgcccag cgcgatgacc tcgtcgtggt cgacgtaccg ggccgtcttg aacacgagga
     3961 acgagtgatc gtcgtagcgc tccagcttcg ggcgctgatg ggcgtgcaat gcgtcctcga
     4021 ccgcgagcgg gtggagatcg aacgtcatgg caaccgcttc gagttcgtcg gcggtcggct
     4081 cgaccagacc gatccacgcg aacgcgtgct cgtcgcgcat ctgtgccacg gcctgacgca
     4141 acgagcacga ctccgcccgg cgctttccgc gttcgtacac cgcgcagtcg acgatcaccg
     4201 ggtccctcgc atggggtgaa cgctaccggt gtccggccct cctcggatcg aggagggccc
     4261 gacccggcga cgacttcagt cgagccgctc gatgatcatc gcgttgctga ggccaccggc
     4321 ctcgcacatg acctgcaggc cgaaccggcc gtcacgttgt tccagcgcgt tgagcagggt
     4381 gccggcgagg cgggcacccg acgcaccgag cgggtggccg agcgcgatgg cgccaccgtt
     4441 gacattgacc ttgtcggggt cggcgccgag ctccttctgc caggcgagca cgacggacgc
     4501 gaatgcctcg ttgacctcga acaggtcgat gtcgtcgatg gtgagcccgg cccgtgccag
     4561 cagcttcttc gtggccggga tgacggcggt cagcatgtag agcgggtcgt cgccctcgac
     4621 agcgagcgag tggatgcggg cacggggcgt gagcccgagc tccttcgcct tggcgtcact
     4681 ggtgacgagc acggcggcgg cgccgtcggt gagctgggac gagttggccg ccgtcgtcac
     4741 ccactggatc tgcgggaagc gctccgacgc ccaatcggcc ccgaaggcag ggcgcagctt
     4801 ggcgaggccg tcgatcgtcg actcgcggat gccctcgtcg ctcgtgacga gctggaggtc
     4861 accgtcggga ccgaccacct cgaggggcac gatctcgtgg tcgaaccggc cgcggtcgcg
     4921 tgccgcggcg gcgttgacct gcgaccggag ggcgaactcg tcgacctcgg cgcgactgag
     4981 cccccacctg gcggcgatga gttccgaggc gatgccctgg gacacgaggc cgtcggggta
     5041 gcgctcgtcg aaggaggggc cgtcgaggtc ggcggctccg agagcggacg agcccatcgg
     5101 gacccggctc atcgactcga cccctgcggc gacgaccacg tcctgggtgc cggacatgat
     5161 cgcctgagca gcgaagtgga tcgcctgctg gctcgaaccg cactggcggt cgatggtcgt
     5221 ggccgggacc cgctcggggt agccggcggc gagcacggcg cgtcgcccga tgttgaacgc
     5281 ctgttcggag acctgggtga cgttgccggc gatgacgtcg tcgatcacct ccggatcgag
     5341 gccgttgcgc tcgacgacgg cgcgcagggt gtgggcgagg agttcgacgg ggtgtgtgct
     5401 cgacaggatc cctcctggct tgccgcgccc gaggggtgtc cgcaccgcat cgatgatcac
     5461 tgcatctgcc atgacgtttc tccgttctca acccagtgag ttgtttgaca gaactaacca
     5521 taggagagct agtcctttga agcaacccac tgctacgatg gccccgaaca tgagaggaac
     5581 gtcatgaagc gaaccagcgt gaaggacatg aactgctcgg tggcgcagtg cctcgaggtc
     5641 atcggcgact ggtggacacc gctcatcgtg cgcgacctcc tcttcggggt cggccgcttc
     5701 gacgagatcc aggagcggct cggcatcagc cgcaacgtcc tcacgcagcg gctcgaactg
     5761 ctcgtcgaga acggcgtcgt cgagcgccag ccgtaccagg acaacccgcc acgcttcgac
     5821 tacgtcctca cccgcaaggg ccgcgacctc tggttcgtca ttcaggcgat gaagcagtgg
     5881 ggcgacacgt gggccgcgcc cgacggcccg ccgatcgagc tcctccacac gacctgcgac
     5941 cacgtcgtga cggtggtacc cagctgcagc gcctgcggtg agcgcctctc ggtcggcgac
     6001 gtccgggccg tcgccggccc cggcgcgacc gaccggagcc cgctccccac gcaccggcgc
     6061 tgacgcgccg acgcccgcgc ctgggattca gtccgggggc agcgaacgaa ggaccaggtc
     6121 gaggaacgcc cgaccggccg gcgacaaggt gccctgccgg tggacgacgc cgatacgccg
     6181 cgagatcgtc gggatgatct cggcgacccg cacatcgccc gacccccgcg ccgccatccg
     6241 ctcggccacg gtcctcggca ggatcgagta gccacctccc gcctcgacga tcgccgtgat
     6301 cacctctcgc aggtcggtct cgatggcgat gcgtggggcg acgccggact ggcggaaggc
     6361 ctcgtcgatc aggcgccgcg tcgacgtacc cggggtggtc gtcacgagcg ggagttcggc
     6421 gagctggggg accgacacga gcgaaccccg ttcgaccgac cggtggtgca cggcgacgta
     6481 ctcctgaccc gacagctcga tcgtcaccag ccgctcgtcc gcgatcggca gctcggtgaa
     6541 gccgatctcg gactcaccgc ccgcgacgtt cgcggcgacg gcgcccacct cgtcgggctc
     6601 gaccagccgc acggtgacgt cggggttccg ggtccggaac gccccgatga agtccgcaac
     6661 cggtgacacg ccgagcgtcg ggagcgacac caggtcgaga cggccgcgcc cgagtccgcg
     6721 gacggcgtcg acggcctcac gggcgatctc gacgtcgtgc aacgcttggc gggccgcggg
     6781 gaggagggct ctccctgccg cggtgagtcg gacctgtcga cccacccggt cgaacagggc
     6841 gacgcccacc tcggtctcga gggccttgat ggcctgggac agcgagggtt gcgcaacgtg
     6901 cagcgcttcg gccgccctcg tgaagccgcc gtggtcgacc acggcgacaa catacgagag
     6961 ctgccggaga tccataggta gatcctatcg tttcaatcca catctcatct tggacgaatc
     7021 gatcggcgtc gtcgaccatg cacccatggc ctcggttccc ctcatctgca ccgccggact
     7081 ggccgtcggt gtgctctacg gcctcttcgg cgtcggctcg gcattcgcca cgccgctcct
     7141 cgccctcctc ggtgtgcccg gtatggccgc agtcgctggc cctctcccgg cgctccttcc
     7201 cgggtcgatg accggtgcgt ggtcctactc ccgcgacggc aaggtcgact ggtgggtcgc
     7261 caagcgcacg gtggccgggg cattccccgc cgccatcgtc ggcgcgatcg ccagccagtg
     7321 ggtgtcgggc cccgtgctcg tcgcgatctc cggcgccgtc ctccttctca tcgggctgcg
     7381 ggtgctccgc cccgtcgacc ccgacgccac ccgacccgtc tgggcccacg accacgccgg
     7441 cctgctcggc gcctcggcga tcgtcatcgg cttctcgtcg ggcctgctcg ccaacggtgg
     7501 cggcttcctc ctcgtgccgc tgttcctcct cgccgtcggc ctcgacatga accgggccac
     7561 gggcaccagc ctcgtggtcg cgtcggcgct caccatcccg acgctcatca cccacgcggc
     7621 catcggtgac atcgactggg ccgtgtcact ggtgttcgcc ctcggcctca tccccggcgc
     7681 ctgggtcggc gccctcgcgt cgaagcgatt cgcgaccgac aagctgcgcg gcgccttcgg
     7741 gttcctgctc gtcgggttcg cggtgtggtt cctcgtgcgc gaggtccccg cgctcctggc
     7801 ctgaccagcg ctgccgtctt gaaggcagcg ttcggctccg gccccggtcg ggtcggtcag
     7861 gaactgagtc gcaccggcag gtgtttgatg ccgttgatga agttcgagcg cagtcgttcg
     7921 ggctcgccga gctgctcgat gttcggcgcc cgatcgacga gcgcctggaa caggagccgg
     7981 atctccatac gggccaggtt cgcgcccagg cagaagtggg ggccaccacc gccgaacgcg
     8041 atgtgggggt tcgggtcccg ggtgatgtcg aaccggtagg ggtcgtcgaa caccgactcg
     8101 tcccggttgg ccgagatgta ccagaggctg accttgtcgc ccgccttcag ctggtgcccg
     8161 tcgtactcgg tgtcggtcgt gacgttccgg cggaagaaca tgaccggcga cgcccagcgc
     8221 aggatctcct cgaccgcgct gtcgatcagc gacgggtcgt cgaggagcag acggtactgg
     8281 tcggggttcg ccaggaacgc ggtcatcccg tgcgacaagg cgttgcgggt ggtttcgttg
     8341 ccggcgaccg acaacaggag gaagaactgg ttgaagtcca tctcggtgag ctggtcacca
     8401 tcgacgactt cgtggatcag cttcgacacg atgtcgtcgc gagggtcggt gcgccgatcg
     8461 gtgccgaggt cgttggcgta catgaacatg ctgaccagcg cctcgttgag gaggtcctcg
     8521 ctcaccgcat actccggatc gtcggtgccg atgaggcggt tcgaccagtc gaagatcttg
     8581 tggcggtcct cgaacggcac acccaggaac tcggcgatcg cctcgagcgg caactcggcc
     8641 gcgatatcga cgacgaagtc acaccggccg ccgtcggccg ccatggctcg atcgacgatg
     8701 cgctcgctca tcaggcgaag gtggtcctcg agcgaacgga tgaccttggg cgtgaagccc
     8761 cggttcagga gcttgcggaa ccgggtgtgg tccggcgggt ccatctccaa catgagccgg
     8821 ccggcgtcgc cgatctcggc gcggcggacg gcctgctcgg gcgtcggctc ctccagttgc
     8881 accaccccgc cgagtgcgtg ctccgaggag aacgtgtgcc agtcgcggtt gagcgccccg
     8941 acgtcgcggt gcgacgtgaa cgcccagaag cccgggccat cgggctcggg gtggtggtgg
     9001 atcggagcct gggcgcggag gcgctcgaac cattcgtgag ggacacgatc tgcgaacacg
     9061 tcgcgactca acaggtcgat gtccgccagg ctcgaagctg acggtgtggt cgtcgcggcc
     9121 atgaggtccc ccctcgggtg cgtgcgctcg gttgcccgag cgtaggccct gacgcatgga
     9181 tctgacctgc aggtcgttcc cgcctacaga cgttctgccc tgcaaatcgt gcggttcgcc
     9241 gatcggatcg cgggacaaaa ccagagcgtc agtgagcggg ggcggggatc cgagcgagct
     9301 ctgagcgcac ctcgccgaac cgggcaccgg cggcggccca atcggcgtag gccgagtcga
     9361 tctcctcggt cgttcgagcc acgaagttcc acgacatcgc gatggtgctc tcgaacgggg
     9421 cgccaccgag gatgacgacg cgtgccggcc cgccgagtgt gaccgacggg cggcccggag
     9481 caaggtacgc ggtcgtgccg accgggacgt gctgcccgtc gacggccggc gaccccgaca
     9541 tgacgagcac ggcgtgttcg aacgtcgggt cgagtggaac ctccgcctcg ccgcgcacga
     9601 cgacgtcggt gccgatcatc ggccagtcgg ctcgggcgct cgaacgcgcc tcggcgagct
     9661 cacccacgag cacggtggcg gtggcctcgc cgagctcgac gaccggcaac tcggccaggt
     9721 gctggaactc gggctcggac caccgggtgg catcgggctg cgcaacccac agctggaggc
     9781 cgtgcatctc acccgcggca tcgtccgggc tctcctcggc gtgggagatg ccgcgaccgg
     9841 cggacatcag gttgagctga ccgggccgga tgagctgttc gctcccgacg ctgtcggtgt
     9901 ggaggacctc gccggcgaac agccacgtga ccgtctggag gccgatgtgg gggtggggcc
     9961 cgacggccat ggccccgtcc cgggtgggag cggggccgag gtcgacggga ccgaagtgat
    10021 cggcaaagca ccacggacca acggtgcgat gggcgcgctt gggcagcagc cgggtgaccg
    10081 gcgtcccgcc gaccgtggcg gcgcggctgt cgagcagttc catcagccca gcggcccgcg
    10141 gtaggggtgg agcccgtcgg tcggcacgag tccgagtagg ccgttgttga agtcgtcgat
    10201 cgcctgggcg agctcggcgc gggtgttcat cacgaacgga ccgtactggg cgatcggctc
    10261 acggatcggc tgaccaccga gcagcagcac ctccatcggc tcaccggcac gctcgccggc
    10321 gccgccaccg agcgtgatcg tgtcaccggc cccgaacacc gccaactggc ccgtcacggc
    10381 cgggcgacgc tcggcgccga cggtgccgcc accaccgagg acgtaggcga gcgcgttgaa
    10441 ctgcggattc cacgggacga cgacctgtgc accgggcgcc acggtgacgt gggccatgac
    10501 gatcggggta tgggtgccgc ccggaccggt gtgaccgtcg acgtcgccgg cgatcaggcg
    10561 gacgagcgcc ccaccgtcgg gcgagctgac cagtgtgacg aggtcggcct cgaggttctg
    10621 gtacgccggg tcgatcatct tgtccttggc cgggaggttc acccacagct gcacgccgtg
    10681 gaacaagccg ccgctgacga ccagctcctc gggcggcgtc tcgatgtgga ggatgccgcc
    10741 accggcggtc atccattggg tcgcaccgtt cgtgatgagc ccaccgccgc cgtgggagtc
    10801 ctggtg
//
LOCUS       DAOSOA010000083        10805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6081_length_10805_cov_6.895535, whole genome shotgun sequence.
ACCESSION   DAOSOA010000083 DAOSOA010000000
VERSION     DAOSOA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6081_length_10805_cov_6.895535"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKY13_07190"
     CDS             <1..797
                     /locus_tag="PKY13_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79755.1"
                     /translation="AVPWSYDRERVERVQEARRHEAEEMLRYGQSPSCLMRLLRSSLD
                     DDGAVECGRCAVCTGLGDPVTLDPAVVRAAVEFLRGVDVVIEPRRQWARGGTGDLPKG
                     NIAADGRAEEGRALCRVGDAGWWPVVQRCEQAGLADDELIAGVFAALKRWPWAQRPTW
                     VTWMPGDDAGIAADVAARVGAAGRLPVHATLALAPGDGAGGANSSFRLAEVWRRVGLA
                     GPVPSVAGPVLLVLDRCDSRWTSTVAAHRLLGAGAGPVLPFALARV"
     gene            complement(815..1576)
                     /locus_tag="PKY13_07195"
     CDS             complement(815..1576)
                     /locus_tag="PKY13_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79756.1"
                     /translation="MTDDQRLSERTYDRVTDGPLMDLHDQLVEAIARQQSTVTPLPST
                     RRRPGAHVRRQLVAAAAAVVVMVGAALFVTSTGDDARVAADVTVEQNANGVRVSIESS
                     VSVEELERELTAAGVDVGVRPMATGPSRVNHFVSISGNSGKLIGGDGKTTNKAQFTKG
                     DEVVLYLGVAAGQRPYDAMTDAWARGESLAGTNLDGTTLAESRDEIDRRAAASETSVS
                     YRLLADGQETTPQSGDVIADATGLSAHSAVIMLDR"
     gene            complement(1569..2117)
                     /locus_tag="PKY13_07200"
     CDS             complement(1569..2117)
                     /locus_tag="PKY13_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT79757.1"
                     /translation="MAATIAGDAAAFGEFYIRHGTRLFAYFRARCANTQDAYDLVSET
                     FASAFQSIADFEPERGEPGAWLFGIAKNKYRHYARRGAVETRGRQRMELTIAPLSPDE
                     LDALEDSLDLSAVRRRVDPAILELPPTLRDAVRLRCIDGRDYDEIATHLGVLPATARK
                     RVSRGLRQLEELLGDNPFEHHD"
     gene            complement(2328..2864)
                     /locus_tag="PKY13_07205"
     CDS             complement(2328..2864)
                     /locus_tag="PKY13_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79758.1"
                     /translation="MHIPSLIRRLVPCIAVVAVLTSMGPTAGATSSDSETEITHCVVS
                     VIDQLESGEYVLSDEECYSDFSSAMESVGLGESADTPAKAEAAALSIQSTLATHYDGA
                     SYTGSSFSVLGINCLGGYVNMSASWDNRVSSTYSSFCPRVRHWTGVNLSGQYQDTLPS
                     GNLSSPVNNTVSSIQYLT"
     gene            2992..3456
                     /locus_tag="PKY13_07210"
     CDS             2992..3456
                     /locus_tag="PKY13_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT79759.1"
                     /translation="MPRTSAGLLLHRNRDGVEVLLVHPGGPFWARKDAGAWSIPKGEY
                     EPGEDPESVARREFREELGADVPTGELMPLGEVRQAGGKRVVAWALRGDLDVSTIVSN
                     TFEMEWPPKSGRMATFPEVDRAEWFSIEVARERILAGQVVLLDRLGDELSAR"
     gene            complement(3461..4915)
                     /locus_tag="PKY13_07215"
     CDS             complement(3461..4915)
                     /locus_tag="PKY13_07215"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOT79760.1"
                     /translation="MSVTRRAFLQGTALAGGGLVLPGLVGCVPENGTGPSTASYLHGV
                     ASGDPDSTSVVLWTRHEPVAGSTTPIPVTWEISTDPNFAQNAASGTANADPSRDWTVK
                     VIPGGLAPGTTYWYRFTSAAGRSPVGRTRTAPSGSVARLRLGFASCSNYAEGYFHGYR
                     HLANRADLDAIVHLGDYIYELGSGTRTVDPTTEAVTLADYRRRYAWHRRDADLAEMHR
                     QHPVICIWDDHEFANDPFVGGANNHQPATEGLWTTRVAAALRAHAEWMPTRVENDLAY
                     RTVRYGNLATLVLADRQRRFIAPQADDGDLYLGRAQFDWLDSTISTNTATWLVLGTQS
                     PFGSITPSMTGEGYGNRDRNRVLDRFLSAPAGNLVVASGDFHKFRALDIARTPSTYNA
                     TTGAGSTAVEFTVGSITSSGTTDATIGSQVRYSNGVSRGYAVMTLTPSLMQTDFYGFD
                     DALKRVPNLPAERWLAGFNSHAGARHLSVAAAPA"
     gene            5033..6322
                     /locus_tag="PKY13_07220"
     CDS             5033..6322
                     /locus_tag="PKY13_07220"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT79761.1"
                     /translation="MPRTGGSDTAVASRPSSFAPLRHRQYRLVWTGSLVSNVGTWMQA
                     AALGYYTAHLTKSAGWSAVVAAGEFAPTALLGPFGGAIADRFARRTVFLTSTLVQAVL
                     AVALTWGMVVGQPGAPVLALFALANGCVFALGFPAYAAILPELVPKEEIGAAVGLSSA
                     AWNLGRIAGPLAGTLLYQQAGIAWVLGVNAVSFLAVVAVLLTLRVPDQARSTGPMLAA
                     IREGFAFVRAQPGLRVTAQALCLNALFVAPFIGLIPAMVEKELGGGKRAVGWLITGQG
                     IGAVITGFVFGRLSETFGVRRVMVASLVGGPVALVAYGLSPSWPWAFVAIIGCGACYF
                     AALSSFSTVANLLAPSDLRGRVLSLNQVILGTVYAISLNIEGQLGDRIGLREVTVGGA
                     LASLVVLLAVRLVRPGSTAVLDPADEAIVASPDPSPA"
     gene            6331..6819
                     /locus_tag="PKY13_07225"
     CDS             6331..6819
                     /locus_tag="PKY13_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT79762.1"
                     /translation="MTHGLTSAARGTLVGVTSGSGSEQYLRDLVLLRRVRDRIDRDFA
                     EPLDVDALARGVNMSAGHLSRQFKQAYGEPPYSYLMTRRIERAMTLLRRGDLNVTDVC
                     FAVGFSSLGTFSTRFTELVGVSPSIYRSEAAHATAGLPSCVAKRVTKPVRNREAPMPA
                     RP"
     gene            6830..7240
                     /locus_tag="PKY13_07230"
     CDS             6830..7240
                     /locus_tag="PKY13_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79763.1"
                     /translation="MDITIHTSFLPHTDPEASLTFYRDTLGFEVRNDVGYEGMRWITV
                     GPVGQPDTNIVLSPPAADPGITDDERRTINEMMAKGTYGMLLLATDDLDGAFAKVKAA
                     GNVEIVEEPTDQPYGVRDCSVRDPSGNMIKIQQR"
     gene            7251..7703
                     /locus_tag="PKY13_07235"
     CDS             7251..7703
                     /locus_tag="PKY13_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT79764.1"
                     /translation="MSAAPRTREQRRRDTEERLAADVDVWVATAFEDGTPYLVPLSFE
                     WDGETLLLATAATGPTGSNLAASGQIRLGLGSTRDVTIIEGDVEMLGIDALAADRADR
                     FAARTGFEPRRPDSPHRWFRVTPRRIQAWREENELADRDLMRDGCWLA"
     gene            7787..10174
                     /locus_tag="PKY13_07240"
     CDS             7787..10174
                     /locus_tag="PKY13_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT79765.1"
                     /translation="MATKSQQPAGTPHIADTHDLIRVQGARVNNLKDVSVEIPKRRLT
                     VFTGVSGSGKSSLVFGTIAAESQRMINETYSSFVQGFMPSMARPDVDVLDGLTTAILV
                     DQERMGANIRSTVGTATDANAMLRILFSRLGDPHIGSPQAYSFNVPSVSGGGAITYEK
                     GGKTVRQKESFSITGGMCPRCDGRGSVSDFDLTALYDENKSINEGAITIPGYSMDGWY
                     GRIFNGCGLFDCDKPIKKFTRKELDALLYQEATKIKVEGINLTFLGLIPQIQKSFLSK
                     DREAMQPHIGAFVDRAVVFTTCPECEGTRLTAGARSSKIAGINIADCCAMQISDLAEW
                     VRGLDEPSVGPLLENLGQTLDSFVSIGLGYLSLDRPAGTLSGGEAQRTKMIRHLGSSL
                     TDVTYVFDEPTIGLHPHDIARMNELLLQLRDKGNTVLVVEHKPEAIAIADHVVDLGPR
                     AGSAGGEIVFEGTVEGLRASDTLTGRHLDDRASIKDTVRTSSEALRIRSANANNLQDV
                     DVDIPLGVLVVVTGVAGSGKSSLIHGSVGGREGVVSIDQGAIKGSRRSNPATYTGLLE
                     PIRKAFAKANGVKPALFSANSEGACPNCNGAGVIYTELGVMATVESPCEVCEGKRFDA
                     SVLDYKLGGKDISEVLSMPMSEALEFFGPGDSSVPPAHKLLVHLNDVGLGYISLGQPL
                     TTLSGGERQRLKLAMHMAEQGGVYVLDEPTTGLHLADVEQLLGLLDRLVDSGKSVIVI
                     EHHQAVMAHADWIIDLGPGAGHDGGKVVFEGTPADLVAAKKKTLTGEHLALYVSR"
     gene            10243..10686
                     /locus_tag="PKY13_07245"
     CDS             10243..10686
                     /locus_tag="PKY13_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79766.1"
                     /translation="MKRWVLIPILAGLSITATGCPEPAPAGWRPPVVDSVVVEPTTIM
                     AGDAFTMTAVVTDDKSVQSVDFGLYTGEPGVLEVQRYLECERDPFVAGAAVTVVARCQ
                     MPADAPLGGWNAVVRATDFEYLPSGPQAAGSKLTSFTVVSGPDPA"
BASE COUNT         1607 a         3437 c         3955 g         1806 t
ORIGIN      
        1 ccgcggttcc gtggtcgtac gaccgtgagc gggtcgagcg ggtccaggag gcccgtcgtc
       61 acgaggccga ggagatgctg cggtacggcc agtcaccgtc gtgtctgatg cggttgttgc
      121 gttcttcgct cgacgacgac ggcgccgtcg agtgcggccg gtgcgccgtg tgcacgggcc
      181 tcggggatcc ggtcaccctc gacccggccg tggtgcgcgc cgccgtcgag ttcctgcgcg
      241 gcgtcgacgt ggtcatcgaa cctcgtcggc aatgggcccg cggcgggacc ggcgatctcc
      301 cgaagggcaa catcgccgcc gatggtcgcg ccgaggaagg gcgtgcgctg tgtcgggtgg
      361 gcgacgcggg ctggtggccg gtcgttcagc gctgtgaaca ggcaggcctc gccgacgacg
      421 aactgatcgc gggggtgttc gccgcgctca agcgatggcc gtgggcccag cgtcccacgt
      481 gggtgacgtg gatgccgggc gacgacgcgg gcatcgccgc cgacgtggcc gcccgggtcg
      541 gcgcggccgg cagacttccc gtgcacgcaa cgcttgcgct cgcccccggc gacggtgcgg
      601 gcggcgccaa ctcgtcgttc cggctggccg aggtctggcg tcgggtcggc ctggccggcc
      661 cggtcccatc cgttgccggc ccggtgctgc tggttctcga ccggtgcgac agccgatgga
      721 cgtccaccgt ggcggcccac cgcttgctcg gcgctggtgc cggcccggtg ctgccgttcg
      781 cgctcgcccg ggtgtgatgc ccgggccagc gtccttaccg atcgagcatg atgaccgcgg
      841 agtgggcgga cagccccgtc gcatccgcga tgacgtctcc tgactgcgga gtcgtctcct
      901 ggccgtcggc caggagacgg taggacaccg acgtttcgga tgcagctgcc cgtcgatcga
      961 tctcgtcgcg gctctcggcc agcgtggtgc cgtcgaggtt cgttccggcg agcgactcac
     1021 cgcgggccca cgcgtcggtc atggcgtcgt agggacgctg acccgcggcg acgccgaggt
     1081 acagcacgac ctcgtcaccc ttggtgaact gggccttgtt cgtcgtcttg ccgtcgccgc
     1141 cgatcaactt gccggagttg cccgagatgg agacgaagtg gttgacgcgc gacgggccgg
     1201 tggccatggg ccgaacgccg acgtcgacgc cggcggcggt gagctctcgt tcgagctcct
     1261 ccacggacac gctgctctcg atcgagaccc tcacgccgtt ggcgttctgc tccacggtga
     1321 cgtctgcggc gacccgggcg tcgtcgccgg tgctcgtgac gaacagtgcc gcgccgacca
     1381 tcaccacgac cgcggcagca gcggcgacga gctgtcgacg cacgtgggcg ccgggtcggc
     1441 gacgcgtgga gggaagcgga gtcacggtcg actgttggcg ggcgatcgcc tcgacgagtt
     1501 ggtcgtggag gtccatcaac ggcccgtcgg tcacccggtc gtaggttcgt tcgctcagtc
     1561 gctggtcgtc agtcatggtg ttcaaaggga ttgtcgccga ggagttcctc gagctggcgg
     1621 aggccacggg agacgcgctt gcgcgccgtc gccgggagga cgccgaggtg cgtcgcgatc
     1681 tcgtcgtagt cgcggccgtc gatgcagcgc aatcgcaccg cgtcgcggag ggtcggaggc
     1741 agttcgagga tggccgggtc gacgcgccgg cgcacggccg agagatcgag cgagtcttcg
     1801 agggcgtcca gttcgtctgg tgagaggggc gcaatcgtca actccatccg ttgtcggccg
     1861 cgcgtctcga cggcgccgcg gcgggcatag tgccggtact tgttcttcgc gatgccgaag
     1921 agccacgcgc cgggctcgcc ccgttcgggc tcgaagtcgg cgatggattg gaatgccgat
     1981 gcgaaggtct ccgacacgag gtcgtaggcg tcctgggtat tagcgcatcg tgcgcggaag
     2041 tacgcgaaga gccgcgtgcc gtgtcggatg tagaactcac cgaacgccgc cgcatcgccg
     2101 gcgatcgtcg cggccaacag agtgtggtcg ggacgcgcgt cgacgggaga acgtcctcgg
     2161 gccggggcgc gagggggctc cgttgtggag cgggtggtcg gttcgacgtc ggtcatgtcc
     2221 gcaactactg cctgtcgcgc acggtaccag caccgaaccg ggttcaccca ggggcgaatg
     2281 cggcggcgtc atttggtgtc agtccattct ggggtcggtc acctcgatca cgtcaggtac
     2341 tgaatcgacg agacggtgtt gttgaccggc gatgagaggt tcccgctcgg cagcgtgtcc
     2401 tggtattgac cgctcaggtt cacaccggtc cagtgccgaa cccgcgggca gaacgagctg
     2461 tacgtcgacg agacccggtt gtcccaggag gcactcatgt tgacgtatcc gccgaggcag
     2521 ttgatgccga gcaccgagaa gctcgaaccg gtgtagctgg caccgtcgta gtgggtggcc
     2581 aacgtcgact ggatcgagag ggcggcagct tcggccttcg ccggcgtgtc cgccgactcg
     2641 ccgagaccga cagattccat cgcggaactg aaatcggagt agcactcctc gtcgctgagg
     2701 acgtactcgc cgctctcgag ttggtcgatg acgctgacca cacaatgggt gatttcggtc
     2761 tccgaatcgg acgacgttgc gccggcggtg ggacccatgg aggtaaggac ggcgaccact
     2821 gcgatgcagg ggacgagccg tcggatcaag gagggaatat gcatgtgagg gctcctgatt
     2881 gctgaacgga tttcgagaat ccggttctca gctcttgccg tgtccgcacc gggtgtgacc
     2941 tcgatgtggt gacaaattcg acgaggtcgc cccggatcta cgctgcgaga catgccgaga
     3001 acgagtgccg ggctcctcct ccatcgcaac cgggacggcg tggaggtgct gctcgtccat
     3061 ccgggcggac cgttctgggc ccggaaggat gccggggcct ggtcgattcc gaagggggag
     3121 tacgaaccgg gggaggaccc cgagtcggtc gcccgtcgtg agttccggga agagctcggc
     3181 gccgacgtgc ccaccggcga gttgatgccg ttgggcgagg tccgtcaggc gggaggcaag
     3241 cgtgtggtcg cgtgggcgct gcgcggggac ctcgacgtgt cgacgatcgt gagcaacacg
     3301 ttcgagatgg agtggccgcc gaagtcgggg cggatggcga cgtttcccga ggtcgaccgg
     3361 gccgagtggt tctcgatcga ggtggcccgt gagcggatcc tggccggcca ggtggtgctg
     3421 ctcgatcgcc tgggcgatga gctgtcggct cggtgagcgg tcaggccggt gcggcagcga
     3481 cgctgaggtg acgcgctccc gcgtgggagt tgaaccccgc gagccagcgt tccgcgggca
     3541 ggttggggac tcgcttcagc gcgtcgtcga acccgtagaa gtcggtctgc atcaggctcg
     3601 gggtgagggt catcacggcg tagccgcggc tgacgccgtt gctgtagcgg acctgtgatc
     3661 cgatggtcgc gtcggtcgtg ccggacgacg tgatcgatcc gacggtgaac tcgaccgcgg
     3721 tcgacccggc cccggtggtt gcgttgtagg tcgacggggt gcgcgcgatg tcgagggcgc
     3781 ggaacttgtg gaagtcgccg ctggcgacga cgagattgcc ggcgggtgcc gagaggaacc
     3841 ggtcgaggac ccggttgcgg tcgcgattgc cgtatccctc gccggtcatg ctgggggtga
     3901 tcgagccgaa cggtgactgg gtgccgagca ccagccaggt ggcggtgttg gtggagatcg
     3961 tgctgtccag ccagtcgaac tgagcccgac cgaggtagag gtcgccgtcg tcggcctgcg
     4021 gcgcgatgaa ccgacgttgg cggtcagcga ggaccagtgt cgcgagattg ccgtagcgga
     4081 cggtccggta ggcgaggtcg ttctcgaccc gggtcggcat ccactcggcg tgggcccgca
     4141 gcgcggctgc gacccgcgtt gtccagagtc cctcggtggc cggctggtgg ttgttggccc
     4201 cgccgacgaa ggggtcgttg gcgaattcgt ggtcgtccca gatgcagatg acggggtgct
     4261 ggcggtgcat ctcggccagg tcggcgtcac ggcggtgcca ggcgtaccgg cgccggtagt
     4321 ccgcgagcgt gacggcctcg gtcgtggggt cgacggttcg ggtgccggac ccgagctcgt
     4381 agatgtagtc gccgagatgc acgatggcat cgagatcggc gcggttcgca aggtgtcgat
     4441 agccgtggaa gtagccctcg gcgtagttcg agcacgacgc aaagccgaga cgaagtcgcg
     4501 ccaccgagcc cgacggtgcg gtgcgcgttc ggccgacggg ggagcggccg gcggccgacg
     4561 tgaagcggta ccagtaggtc gtaccgggcg cgagcccgcc ggggatcacc ttcacggtcc
     4621 agtcgcggga tgggtccgcg ttggcggtgc ccgaggcggc gttctgggcg aagttcggat
     4681 cggtggagat ctcccaggtc actgggatcg gggtggtcga gccggcgacc ggctcgtggc
     4741 gggtccagag caccaccgat gtggaatccg gatcgccgga ggcgacaccg tggaggtacg
     4801 acgcggtcga ggggccggtg ccgttctcgg ggacgcagcc cacgagtccg ggcagcacga
     4861 gcccacctcc ggccagtgcg gttccctgca ggaacgctcg acgtgtgaca ctcatcccgg
     4921 gctccccagc tcgctgtcgc tcagggtacc gagggtcccc catgctggcg actccgtgac
     4981 agcgccgcag gtcccgtcgc gatcgccgtg ggtcgcgtgg gaagctgggc ggatgccacg
     5041 gacgggagga tcggacactg cggtcgcgtc gcgaccgtcc tcgttcgccc cgctgcggca
     5101 ccgtcagtac cggctggtct ggaccgggtc gctggtgtcg aacgtcggta cctggatgca
     5161 ggcggcagcc ctcgggtact acacggcaca cctcaccaag agcgccgggt ggtcggcggt
     5221 cgttgccgcc ggagagttcg ccccgacggc gctgctcggc ccgttcggcg gagcgatcgc
     5281 ggaccgtttc gcccgacgca cggtgttcct cacgtcgacg ctggtgcagg cggtcctcgc
     5341 cgtcgcgctc acgtggggca tggtcgtcgg ccagcccggc gcgcccgtgc tggccctgtt
     5401 cgcgttggcg aacgggtgtg tgttcgcgct cgggttcccc gcgtacgcgg cgatcctgcc
     5461 ggagctggtg ccgaaggagg agatcggagc ggcggtcggg ttgagctcgg cagcgtggaa
     5521 cctggggcgg atcgccggcc cgctcgccgg cacgttgctc tatcaacagg ccggaatcgc
     5581 gtgggtgctg ggcgtcaatg cagtgagctt cctcgcggtc gtcgccgtcc tgctcacgct
     5641 gcgggtacct gatcaggcgc gctcgacggg gccgatgttg gctgcgatcc gtgaggggtt
     5701 cgcgttcgtg cgggcgcagc ccggtctgcg ggtgaccgcc caggcgttgt gcctcaacgc
     5761 cctcttcgtc gccccgttca tcgggctgat cccggcgatg gtcgaaaagg agctcggtgg
     5821 cggcaagcgg gcggtgggct ggctgatcac gggccagggc atcggagccg tgatcacggg
     5881 cttcgtgttc gggcggttgt cggagacgtt cggtgtccgg cgcgtcatgg tggcgtcact
     5941 cgtcgggggt ccggtggcgc tcgtggccta cggtctcagc ccgtcgtggc cgtgggcgtt
     6001 cgtcgcgatc atcggatgcg gggcgtgtta cttcgcagca ctgtcgagct tctcgaccgt
     6061 cgccaacctg ttggcgccgt ccgacctgcg cggccgagtg ttgtcgctca accaggtgat
     6121 cctgggcacg gtgtacgcca tctcgctcaa catcgagggc cagctgggcg accggatcgg
     6181 cctccgggag gtgacggtgg gcggtgcgct cgccagtctg gtggtgctct tggccgtgcg
     6241 ccttgttcgc cccggctcga cggcggtgct cgatccggca gacgaggcca tcgtggcgag
     6301 ccccgacccg tcgccggcgt gatggtggcc atgacccatg gcttgaccag tgcggcgcgt
     6361 ggcacgctcg tgggggtgac gagcggttcg gggtcggagc agtacctgcg agatctggtg
     6421 ttgctgcgac gcgttcgcga tcgcatcgac cgcgacttcg ccgagccact cgacgtcgac
     6481 gccctcgctc ggggcgtgaa catgtcggcc ggccacctca gccgccagtt caaacaggcc
     6541 tacggcgagc ctccgtacag ctacctcatg acccgccgca tcgaacgagc gatgacgctc
     6601 ttgcggcgcg gcgacctgaa cgtcaccgac gtctgcttcg ccgtcgggtt ctcgtcgctc
     6661 gggaccttca gcacccggtt cacggaactg gtcggggtgt cgcccagcat ctatcgcagc
     6721 gaggcggcgc acgccacggc cggcctgccg tcgtgcgtgg cgaagcgggt caccaaaccg
     6781 gtcaggaatc gagaagcgcc aatgccggcc cgtccgtagc gttgccccca tggacatcac
     6841 cattcacacg agcttcctcc cgcacaccga tcccgaggcg tccctcacct tctatcgcga
     6901 caccctcggg ttcgaggtgc gcaacgacgt cggatacgag ggcatgcgtt ggatcacggt
     6961 cggcccggtc ggccagcccg acaccaacat cgtgctgtcg ccgcccgcgg ccgacccggg
     7021 catcaccgat gatgagcgtc gcacgatcaa cgagatgatg gcgaagggca cctacgggat
     7081 gctgctgctc gcgaccgacg acctcgacgg cgcgttcgcc aaggtgaagg cggcgggcaa
     7141 cgtcgagatc gtcgaggagc cgaccgacca gccgtacggc gtgcgcgact gctcggtgcg
     7201 tgacccctcc ggcaacatga tcaagatcca gcagcgctga ggcgggcgct gtgtccgctg
     7261 ccccccgaac ccgcgaacag cgtcgccgtg ataccgagga acgcctcgcg gccgacgtcg
     7321 acgtgtgggt cgccaccgcg ttcgaggacg gaacgccgta cctcgtgccg ctgtcgttcg
     7381 aatgggacgg cgagacgttg ctgttggcca ccgccgccac cggcccgacc gggtcgaacc
     7441 ttgcggcgag tgggcagatc cgactcgggt tgggctcgac ccgtgacgtg acgatcatcg
     7501 agggcgacgt cgagatgctt gggatcgacg cactcgctgc ggaccgtgcc gaccggttcg
     7561 ctgcgaggac gggcttcgaa ccgcggcggc cggactcgcc ccaccgctgg ttccgcgtga
     7621 cgccccgccg gatccaggcg tggcgcgagg agaacgagct ggccgaccgc gacctcatgc
     7681 gtgacggctg ctggctggcc tgaccgcccg cccggcgcca cagccgctgc gcgaaactgc
     7741 tggcccgcgt ttcgcggtcc ggccgacaga tggagagacg tacaccatgg cgacgaagtc
     7801 ccagcagccg gccggaacgc cgcacatcgc cgacacccac gacctgatcc gcgtgcaggg
     7861 cgcgcgggtc aacaacctga aggacgtgag cgtcgagatt ccgaagcgac ggctgacggt
     7921 cttcaccggc gtgtccggtt cgggcaagag ctcgctcgtc ttcggcacga tcgcggcgga
     7981 atcgcagcgc atgatcaacg agacctacag ctcgttcgtg cagggcttca tgccgtccat
     8041 ggcgcggccc gacgtggacg tgctcgacgg gctgacgacg gcgatcctcg tcgaccagga
     8101 acgcatgggt gccaacatcc ggtcgacggt cggcacggcg accgacgcca atgcgatgct
     8161 gcggatcctg ttcagccggc tcggtgatcc acacatcggg tcgccgcagg cgtactcgtt
     8221 caacgtcccg tcggtgagcg gaggaggggc gatcacctac gagaagggtg ggaagacggt
     8281 ccgccagaag gagagcttct ccatcacggg cggcatgtgt ccccggtgcg acggccgggg
     8341 gtcggtgagc gacttcgatc tgactgcgct ctacgacgag aacaagtcga tcaacgaggg
     8401 cgcgatcacc attccgggct acagcatgga cggctggtac ggccgcatct tcaacggctg
     8461 tgggctcttc gactgcgaca agccgatcaa gaagttcacg aggaaggagc tcgacgccct
     8521 cctctaccag gaggcgacca agatcaaggt cgagggcatc aatctcacgt tcctcggcct
     8581 gatcccgcag atccagaagt cgttcctgtc gaaggaccgc gaggcgatgc agccccacat
     8641 cggggcgttc gtcgatcgtg ccgtcgtgtt cacgacgtgc ccggagtgcg agggcactcg
     8701 gctgacggcc ggggcgcgct cgtcgaagat cgcgggcatc aacatcgcgg actgctgtgc
     8761 gatgcagatc agcgatctcg ccgaatgggt gcgcgggctc gacgagccgt cggtcgggcc
     8821 gctgctcgag aacctcggtc agacgctcga ctccttcgtc tcgatcggtc tcggctacct
     8881 gagcctcgat cgaccggcgg gcacgttgtc gggtggtgag gcgcagcgca ccaagatgat
     8941 ccgccacctg gggtcgtcgc tcaccgacgt gacctacgtg ttcgacgagc cgacgatcgg
     9001 gctgcaccct cacgacatcg cccgcatgaa cgagctgttg ctccagctcc gcgacaaggg
     9061 caacaccgtg ttggtcgtcg agcacaagcc cgaggcgatc gcgattgcgg accatgtcgt
     9121 cgacctcggt cctcgcgcag gcagcgctgg aggcgagatc gtgttcgagg gcacggtcga
     9181 gggtctccgg gcgagcgaca ccctgacggg ccgtcacctc gacgaccgcg cgtcgatcaa
     9241 ggacacggtt cgcacgtcca gcgaggcgct gcggatccgc agtgcgaacg cgaacaacct
     9301 gcaggacgtc gacgtcgaca tcccgctcgg cgtgctcgtg gtcgtgaccg gtgtggccgg
     9361 gtcgggcaag agctcgttga tccacggttc ggtgggcgga cgtgagggtg tcgtgtcgat
     9421 cgaccagggc gcgatcaagg gttcgcgtcg cagcaacccg gcgacctata ccggcctgtt
     9481 ggagccgatc cgcaaggcgt tcgccaaagc caacggtgtg aagccggcgc tgttcagcgc
     9541 caactccgag ggtgcatgtc ccaactgcaa tggcgccggt gtgatctaca ccgagctcgg
     9601 ggtgatggcg accgtcgaga gtccatgcga ggtgtgcgag ggcaagcgct tcgacgcttc
     9661 cgtgctcgac tacaagctcg gcggcaagga catcagcgag gtgctgtcga tgccgatgag
     9721 cgaggcgctc gagttcttcg ggcccggcga ctcatcggtg cccccggcgc acaagctgct
     9781 cgtgcacctc aacgacgtcg ggttggggta catcagcctg ggccagccgc tgaccacgct
     9841 gtcgggtggt gagcgtcagc gcctcaagct cgcgatgcac atggcggagc agggcggggt
     9901 gtacgtcctc gacgagccga ccaccggcct ccatctcgcc gatgtcgagc agctgctcgg
     9961 gctcctcgat cggctggtcg actccggcaa gtcggtgatc gtgatcgagc accatcaggc
    10021 ggtcatggcg cacgcggact ggatcatcga cctcggtccg ggggccggcc acgacggcgg
    10081 caaggtcgtg ttcgagggca cgcccgccga tctcgtcgcg gcgaagaaga agaccctcac
    10141 cggcgagcat ctcgcgctgt acgtcagcag gtgagggagc cggccgaacg tgcggtacgg
    10201 tcggccgacg ccggacgttc gttcggtgtg gagggggctc cgatgaagcg atgggtgctg
    10261 attccgatct tggccgggtt gtcgatcacg gcgacgggat gtcccgagcc ggctccggct
    10321 gggtggcggc cgccggtggt ggattcggtc gtcgtcgagc ccacgacgat catggctggt
    10381 gacgcgttca cgatgactgc ggtggtcacc gacgacaagt ccgtacagag cgtcgacttc
    10441 ggcctctaca ccggtgaacc cggggtgctc gaggtgcagc ggtacctcga gtgcgaacgc
    10501 gacccgttcg tggcgggcgc tgccgtgacg gtggtagcgc ggtgccagat gcccgccgac
    10561 gcgcccctgg gcggttggaa cgcggtcgtt cgggcgaccg acttcgagta cctcccgtcg
    10621 ggcccgcagg ccgccggttc caagctcacc tcgttcaccg tggtcagcgg ccccgatcct
    10681 gcctgacgcg gttcatcgga gcacgtcgtc aggttcggac ggaatcggca ccgggaacct
    10741 gcgccacgct tggtggagga gatgggactc gaacccacga ccccctgctt gcaaagcagg
    10801 tgctc
//
LOCUS       DAOSOA010000084        10754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6118_length_10754_cov_6.667726, whole genome shotgun sequence.
ACCESSION   DAOSOA010000084 DAOSOA010000000
VERSION     DAOSOA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6118_length_10754_cov_6.667726"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKY13_07250"
     CDS             <1..357
                     /locus_tag="PKY13_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79767.1"
                     /translation="RVHPAARSRVGAAVASVGVFATLGIGLAVAAQTGTAAASISTTD
                     TTTTDAAMAGDSSDTSVDWSATEATTTTTTTTEATTTTTAAPTTAAASRQTSSSTATA
                     GSAGSAGSANATSGGS"
     gene            363..1295
                     /locus_tag="PKY13_07255"
     CDS             363..1295
                     /locus_tag="PKY13_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT79768.1"
                     /translation="MGTHERLLPAMGSMAHIVVVGPDDTSSTPHAPSAPTLVDRAVTR
                     LRELEGRWSRFLPGSEVSRLNAAIASGEEPPVLSDDTLLLLERAAEGFRVTHGRFDAT
                     RLDAVTAAGYVRSLDRHGPSPLATTGSTPEVGFDPGGIGKGLAADIITAELMSDGAAG
                     ALVSIGGDLRVRGIAPGGGAWRIDIEDPRSDNGAALATVTLEDGAVATSSQLKRRWSD
                     EQGRPVHHLIDPATGTPSTSPVLAATVIAAEAWQAEVLTKVAFLDAFGEAALSDDAGF
                     ALIESLSAAALIVTEDLVATTARWERFALEEATR"
     gene            1292..1876
                     /locus_tag="PKY13_07260"
     CDS             1292..1876
                     /locus_tag="PKY13_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOT79769.1"
                     /translation="MNGQLWWYAARSGGYVAWGLLSASVIWGLMLSGKVRPGHVRPNW
                     ILDLHRFLGGLAATFTAVHVGTIMLDSYTSFGLADVLVPFTSSWHPGAVAWGIVGMYL
                     LAAVEITSLLKKRLAHRIWKRVHALSLPLFVTATVHLLTAGTDAVNPISLVAVASVSV
                     SVTALTIWRVAGLVGGSGTSSGRQATARSAMIRG"
     gene            1873..4167
                     /locus_tag="PKY13_07265"
     CDS             1873..4167
                     /locus_tag="PKY13_07265"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOT79770.1"
                     /translation="MTGTLRIGTTIADSTPEFAPRPTPPAGAPNVVVIVLDDLGFGQL
                     GCFGSDLSTPHIDDLADRGLRYHRFHVTALCSPTRAALLTGRNHHSVGMGFLTDVPIG
                     FPGYDGRIPKSAATLPRILRDNGYATFAVGKWHLAPRWEQSAAGPFGQWPLGLGFERF
                     YGFLGGDANQWTPELVSDNGFIDPPARPEDGYHLTDDLVDRSRRLILDQQHARPGAPF
                     LLYFAPGAMHAPHQAPAEWIDRYRGHFDQGWDRWRDGVFAKQVAAGIVPAATELTPRP
                     PWVAEWDSLSHDQRRLYARQMECFAGFLSHTDDQIGRLMGTLRELDVLDNTVVVVISD
                     NGTSAEGGPIGSHNEHRFTHDRLDDFDDSLARIDDLGGFRAYNHYAWGWAWAGNTPMR
                     LWKRYTWLGGVRTPLIVSWPDRIGPDSAGSIRSQFCHAVDITPTLLDAIGITAPSEVD
                     GVPQQPMAGVSLLPTFDDPDLADPRSTQYFEMLGSRAIFHDGWKATTDHIGNQLSIER
                     ELVEGSHDFDDDHWALFDLRSDFSESTDVSAEHPDVVEDLQRRWWAEAEAHHVLPLDD
                     SLIGRAVALEPNPNPMRWKSYYRPGGGPIAEDALPGMGGGFRLHADIDVAADASGIVC
                     ALGDWNNGWAVYLLDGRPNVAFNLFGEAHVARSGDTLPGGAVRLTVGFRRTHPGGGPV
                     TLLVDDVEVASVELPIDLPFRWQIGGTGLRLAADHGFPVCDDYATPFPLDGEVALVTI
                     EIPAFAPRPRPDDRTTVAEALHRE"
     gene            4191..5195
                     /locus_tag="PKY13_07270"
     CDS             4191..5195
                     /locus_tag="PKY13_07270"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOT79771.1"
                     /translation="MWHHLAGQVERCDECRFDGSAYTVDDVSGTLRNLAEWAGHAMGG
                     IEPTDLNRRPTPTTWSPAEYLRHTKRVVWSMAGLAEMALAESGLAVAGSPPDNASADD
                     PPAIVDVYREFVRLDEEAQRLHLLWAKASADERARTIDINGSTADVAGIVRHAAHDAT
                     HHLADIGRGIVALGLGTPDAVGCVDLIHASGGGVPKLTVPQAIVGYRGVGGDRQATRK
                     HHGRVWQAICLWSTEVIDTLAAEGHPVAPGFAGENFTLSGIDWATLRPGARLEIGGRD
                     GVVLETTAWATPCKTLNDVFTNRDFRRIDVLRDPGASRLYAKVLRDGVVRAGDTVRVH
                     "
     gene            complement(5214..5618)
                     /locus_tag="PKY13_07275"
     CDS             complement(5214..5618)
                     /locus_tag="PKY13_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT79772.1"
                     /translation="MSAERQLISSGSPWELTVGYSRAVRFGSRVFVAGTAPKFDDGVC
                     PPDAGLQARRCLEIIGQALDDAGATFGDVVRTRIYLIDAADFEAVGSVHGEFFHDVRP
                     ANTTVVVAALLDAAWKVEIEVEAIISKDRSIH"
     gene            complement(5643..6275)
                     /gene="recR"
                     /locus_tag="PKY13_07280"
     CDS             complement(5643..6275)
                     /gene="recR"
                     /locus_tag="PKY13_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT79773.1"
                     /translation="MADYAHSIQELIDAFGRLPGIGPKSAQRITFHLLKVDKAESQRL
                     VSAISSAVEKVSFCTRCYNLAEGDLCAICSDDQRDAHQLCVVEEPRDVVAIERTREYR
                     GLYHVLGGAIDHLQGVGPDQLKIKELLGRVGAEGITEVILCTNPNIEGEATALYLQRV
                     LDVPGLTVSRIASGLPVGGDLEYADELTLGRALEGRRVVGEPVRPDVSAP"
     gene            complement(6275..6685)
                     /locus_tag="PKY13_07285"
     CDS             complement(6275..6685)
                     /locus_tag="PKY13_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOT79774.1"
                     /translation="MTDGALPENVNDPVEVVGDDAAPGGLGGLDLGSLLGAAQNMQAQ
                     MLESQQRIGATVVEGQSGGGAVTIAVTGGFEFQSVTISPEVLPDGTDPDDRAMLEDLV
                     LAALRDATSKVVALQSEANPLAGLGDLGGLFGGA"
     gene            complement(6682..8622)
                     /gene="dnaX"
                     /locus_tag="PKY13_07290"
     CDS             complement(6682..8622)
                     /gene="dnaX"
                     /locus_tag="PKY13_07290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOT79775.1"
                     /translation="MAHQSLYRRYRPRRFADVRGQDHVVKALRNAVAQGTEGHAYLFS
                     GPRGTGKTSTARILAKALNCPNLVDGEPCGECESCLSIEHGNSYDLFELDAASNNGVD
                     AMRELISRTVVGSPGRNKVYILDEVHMLSPAASNALLKTLEEPPEHVCFVLATTDPQK
                     VLPTIRSRTQHFEFSLLSATELADYVRWIREDAGLTFDDAAVDHVVRQGRGSARDTLS
                     ALDMVVAAGGVTDRAESVEEIMTALVDRDTARVMIAVSDAVAQGREPRVLAEQLLVSL
                     RDAFLASVHAHLEHLSDSDQAQMADLAGRLGTATITRALESLGLAIVDMRQAADPRVP
                     LEVALIRLTTPSADTSVQALVERIDDLERRLARGIPAGAAPAAPAPATSPGSDTPAPA
                     TPVVEPHPEPAPTSGAASSPAEIRAQLAARKGDAPSAPTRPSPPKPPTRPGAPSRPGS
                     ADPSPSAPAATPAPAPAPSSAAPPAPVALVSEPSAPTTAPAPAPAAGGGTVTLEQLAA
                     AMTESVLPTLSGMTKSLYGGGRFVGEADGKFQFALDNVPDRVLPRAQAALADVEAALS
                     VRVGQPVSVHLTNGGEGSSSAPSSSGTPAPTPVVAADTDQDERDHIDVSDLEDATDVS
                     TSGVDKLVQAFPGAELIEQETP"
     gene            8755..9600
                     /locus_tag="PKY13_07295"
     CDS             8755..9600
                     /locus_tag="PKY13_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOT79776.1"
                     /translation="MSFPVAPPPATAPSAPPGWYRDHHNERWLWWDGWQWITPPEPTP
                     VSDDRTWFPRTPTLQLPAAVLGIVLIVVLTIGTRVAALLPETPEALVQLALFAVSTFG
                     MPLAAWYGSRRWGTGRFVDDLGFRFRWIDVPLGIGGAIALTISLMVINIVAHVLGAPS
                     GSNLTEVSERGRDVVTFATLFVTAGLIAPLTEELLFRGLIQRGLSSRWTPWIAVAMQA
                     AVFGAAHITPSEGWGNADLIVSLAVMGFGLGAIARLTGRLGTSIVAHALFNCIQLALL
                     WLTLS"
     gene            9597..10286
                     /locus_tag="PKY13_07300"
     CDS             9597..10286
                     /locus_tag="PKY13_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT79777.1"
                     /translation="MTAATLVLLHPLPLDGSIWAREISSLTPRSIAPTLYAYGDTLDA
                     WAVSVIEEAGDGPLVVVGNSVGGSCALDVVRLDPGRVQLLVLVGTKAAHRPEPRVRDE
                     ALELLARDGIAAAWDRYWAPLFAADADPTVVAAARSVALRQTVDDLAVGVRAFHGRPD
                     RSDLARSIDVPTLVVCGEHDPLSATAPAFAGGLRRGRCRIVEGAGHYVPVERPAELAA
                     LIAAELDSVAW"
     gene            complement(10287..10385)
                     /gene="ffs"
                     /locus_tag="PKY13_07305"
     ncRNA           complement(10287..10385)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKY13_07305"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            10479..10563
                     /locus_tag="PKY13_07310"
     tRNA            10479..10563
                     /locus_tag="PKY13_07310"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10513..10515,aa:Ser,seq:gga)
BASE COUNT         1552 a         3840 c         3726 g         1636 t
ORIGIN      
        1 cgggttcacc ccgccgcccg gtcacgggtc ggtgcggccg tcgccagcgt cggggtcttc
       61 gcaaccctcg ggatcggcct cgcggtcgct gcccagaccg ggaccgccgc ggcgagcatc
      121 tcgacgaccg acaccacgac gactgacgcg gcgatggccg gtgacagctc cgacacctcg
      181 gtcgactgga gtgccaccga ggccaccacg actaccacca ccaccaccga ggccacgacc
      241 accacgaccg ccgcgccgac caccgccgcg gcgtcccgcc agacgtcctc gtcgaccgcc
      301 acggcggggt ccgcgggatc ggccggctcg gcgaacgcca cgagcggagg gagctgaccg
      361 cgatgggcac ccacgaacgc ctgctcccgg cgatgggctc gatggcgcac atcgtcgtcg
      421 tcggccccga cgacacatcg tccaccccgc acgcgccgtc ggccccgacg ctcgtcgacc
      481 gcgccgtcac ccgcctccgc gagctcgaag gccggtggag ccggttcctt cccgggagcg
      541 aggtgagccg gctgaacgcc gccattgcct ccggcgagga accgccggtg ctgtcggacg
      601 acaccctgct cctgctcgaa cgcgctgcgg aggggttccg ggtgacccac ggccggttcg
      661 acgccacccg gctcgacgcc gtcaccgccg ccggatacgt ccggtccctc gatcgccacg
      721 gtccgtcgcc actcgcgaca acagggtcga ctcccgaggt ggggttcgac ccgggcggca
      781 tcggcaaggg cctggcggcc gacatcatca ccgccgaact catgagcgac ggcgccgccg
      841 gcgcgctcgt ctcgatcggg ggcgacctcc gggtacgcgg catcgctccc ggcgggggcg
      901 cgtggcggat cgacatcgag gacccacggt ccgacaacgg cgccgcgctc gccaccgtca
      961 cgctcgagga cggtgccgtc gcgaccagct cccaactgaa gcggcggtgg tccgacgagc
     1021 agggccgccc cgtccaccac ctgatcgacc cggccacggg aacgccctcg acctcgccgg
     1081 tcctcgccgc gaccgtcatc gccgcggagg cctggcaggc cgaggtgctg accaaggtcg
     1141 ccttcctcga cgccttcggc gaggctgcgc tgtccgacga cgccggcttc gcactgatcg
     1201 aatcgctcag cgccgcggcg ctcatcgtca ccgaggatct cgtcgccacc accgcccgct
     1261 gggagcggtt cgcactcgag gaggcgaccc gatgaacggt caactctggt ggtacgccgc
     1321 ccggtccggc gggtacgtcg cgtgggggct gctcagcgcc tccgtcatct ggggcctgat
     1381 gctgtcgggc aaggttcggc cgggccacgt acgccccaat tggatcctgg acctgcaccg
     1441 gttcctcggc ggactggcgg cgaccttcac cgccgtccac gtcgggacga tcatgctcga
     1501 ctcctacacc agcttcggtc tggccgatgt gctggtgccc ttcacgtcgt cgtggcatcc
     1561 cggcgccgtc gcctggggca tcgtcggcat gtacctgctg gcggccgtgg agatcacgtc
     1621 gctcctcaag aagcgactcg cccaccggat ctggaagcgg gtccacgccc tgtcgctccc
     1681 cctgttcgtg acggccaccg tccatctcct gacggccgga accgacgcgg tcaaccccat
     1741 ctccctcgtc gccgtggcca gcgtcagcgt cagcgtcacg gcgctcacca tctggcgggt
     1801 cgccggactc gtcggcgggt cgggcacctc gtcgggccgg caggccaccg cccgatcagc
     1861 catgatccgg ggatgaccgg aaccctccgc atcggcacca ccatcgccga ctcgacgccc
     1921 gagttcgccc cgcgaccgac cccgcccgcc ggggcaccca acgtcgtcgt gatcgtgctc
     1981 gacgatctcg gcttcgggca gctcggctgc ttcggctcgg acctgtcgac ccctcacatc
     2041 gacgacctcg ccgaccgcgg actccgttac caccggttcc acgtgaccgc gttgtgctcg
     2101 ccgacccggg cggcgctgct gaccggccgc aaccaccact ccgtcggcat ggggttcctc
     2161 accgacgtgc cgatcgggtt ccccggctac gacggacgca tcccgaagtc cgcggcgacg
     2221 ctgccgcgga tcctccgtga caacgggtac gcgacgttcg ccgtcggcaa gtggcacctc
     2281 gctccccgct gggagcagag cgcggccggc ccgttcggac agtggcccct gggcctgggg
     2341 ttcgaacgct tctacggctt cctcggcggc gacgccaacc agtggacccc cgagctcgtg
     2401 tcggacaacg gcttcatcga cccaccggcg cgacccgagg acggctacca cctcaccgac
     2461 gacctcgtcg atcgatcccg ccgattgatc ctcgaccagc agcacgcccg ccccggcgcg
     2521 cccttcctgc tctatttcgc acccggcgcg atgcacgccc cccaccaggc gcccgccgag
     2581 tggatcgacc ggtaccgggg ccacttcgat cagggctggg accggtggcg cgacggcgtg
     2641 ttcgcgaagc aggtcgccgc cgggatcgtg ccggcggcga ccgaactcac cccacgaccc
     2701 ccgtgggtcg ccgagtggga ttcgctcagc cacgaccagc gccgcctcta cgcccgccag
     2761 atggagtgct tcgccggctt cctctcccac accgacgacc agatcggccg cctgatgggc
     2821 acgctccgcg aactggacgt gctcgacaac accgtcgtcg tggtcatcag tgacaacggc
     2881 accagcgcgg agggcggacc catcgggtcg cacaacgagc accgcttcac ccacgaccgg
     2941 ctcgacgact tcgacgattc gctggcccgc atcgacgacc tcggtggctt ccgcgcctac
     3001 aaccactacg cgtggggttg ggcgtgggcc ggcaacacgc cgatgcgact gtggaagcgg
     3061 tacacgtggc tcggcggggt ccggaccccg ctgatcgttt cgtggcccga ccgcatcggg
     3121 ccggattcgg ccggctcgat ccggagtcag ttctgccacg cggtggacat cacgccgacg
     3181 ctgctcgacg ccatcggtat caccgcgccg tcggaggtgg acggtgtccc ccaacagccg
     3241 atggccggcg tcagcctgct gccgaccttc gatgaccccg atctggcgga cccccgatcc
     3301 acgcagtact tcgagatgct cggcagtcgc gccatcttcc atgacggatg gaaggcgacc
     3361 accgaccaca tcggcaacca gctcagcatc gaacgcgaac tggtcgaggg aagccacgac
     3421 ttcgatgatg accactgggc gctgttcgac ctgcggtccg acttctcgga gtcgaccgac
     3481 gtgtcggccg agcatcccga cgtggtggag gacctccagc gccggtggtg ggccgaggcc
     3541 gaggcccacc acgtgctgcc gctcgacgac agcctcatcg gacgagctgt cgctctcgag
     3601 cccaacccca accccatgcg ctggaagtcc tactaccggc ccggcggcgg tccgatcgcc
     3661 gaggatgccc tccccggcat gggcggcggc ttccgactgc acgccgacat cgacgtcgcc
     3721 gcggacgcgt cgggaatcgt gtgcgcgctc ggcgactgga acaacggctg ggccgtgtat
     3781 ctgctcgacg gccgacccaa cgtcgcgttc aacctgttcg gcgaggccca cgtggcccgg
     3841 tccggcgaca cgttgcccgg gggcgccgtg cgcctcaccg tcgggttccg ccggacccac
     3901 cccggcggtg gtccggtcac gctcctcgtc gacgacgtcg aggtggcgag cgtcgaactg
     3961 ccgatcgatc tgccgttccg ttggcagatc ggcggcacgg ggctgcggct cgccgccgac
     4021 cacgggttcc ccgtgtgcga cgactacgcg acaccgttcc cgctcgacgg cgaggtcgcg
     4081 ctcgtcacca tcgagatccc ggcgttcgct ccccggccgc gccccgacga ccgcaccacg
     4141 gtcgccgagg cgctccaccg cgaatagccg ctccggtagc gtcggtgccc atgtggcacc
     4201 acctcgcagg ccaggtcgag cgatgcgacg agtgccgctt cgacggctcc gcgtacaccg
     4261 tcgacgacgt gtcgggcacg ctccggaacc tcgcggagtg ggcgggtcac gccatgggag
     4321 gcatcgagcc caccgacctc aaccgtcggc cgacacccac gacctggtcg ccggcggagt
     4381 acctccgcca caccaagcgg gtcgtgtggt cgatggcagg tcttgccgag atggcactgg
     4441 ccgagtcggg tctcgccgtc gctggatcgc cgcccgacaa cgcctccgcg gatgatccgc
     4501 cggcgatcgt cgacgtgtac cgcgagttcg tccggctcga cgaggaggcg cagcggctcc
     4561 atctgctctg ggccaaggcg tccgccgacg agcgggcacg gacgatcgac atcaacggat
     4621 cgacggccga cgtcgccggt atcgtccgcc acgcagcgca tgacgccacc caccacctcg
     4681 ccgacatcgg gcgcggcatc gtcgcgttgg ggctcggcac acccgacgcg gtcggttgcg
     4741 tcgacctgat ccacgcatcg ggtggcggtg tccccaagct caccgtgccc caggcgatcg
     4801 tcggctaccg cggcgtcggc ggcgatcgcc aggcgacccg caagcaccac ggccgcgtgt
     4861 ggcaggcgat ctgtctgtgg tcgaccgagg tcatcgacac gcttgcggca gaagggcacc
     4921 cggtcgcacc cgggttcgcc ggcgagaact tcacgctctc gggcatcgac tgggcgacgc
     4981 tgcggcccgg agcacgcctc gagatcggcg gacgcgacgg ggtcgtgctc gagaccacgg
     5041 cgtgggcgac gccgtgcaag acgctcaacg acgtgttcac gaaccgcgac ttccggcgca
     5101 tcgatgtgct ccgcgatccc ggagcgtcac gtctctacgc caaggtgctc cgcgacgggg
     5161 tcgtgcgggc cggcgacacc gttcgggtgc actgacccga acgtcaggcg acgtcagtgg
     5221 atcgaacggt ccttgctgat gatcgcctcg acttcgatct cgaccttcca cgcagcgtcg
     5281 agcaacgctg cgaccaccac ggtggtgttg gccggacgga cgtcgtggaa gaactcgccg
     5341 tgcacgctgc cgacggcttc gaagtcggcg gcatcgatca ggtagatgcg cgtccggacg
     5401 acgtcaccga acgtcgcgcc cgcgtcgtcg agcgcttggc cgatgatctc gagacagcgg
     5461 cgggcctgga ggcctgcatc ggggggacag accccgtcgt cgaacttcgg ggcggtgcct
     5521 gccacgaaga cccggctgcc gaaccggact gcccggctgt agccgaccgt gagctcccag
     5581 ggagagccgg aagagatgag ctggcgttcc gcagacatga gcagcaccct accgttgtgg
     5641 ggtcaggggg ccgagacgtc ggggcgaacc ggctcaccga ccacccggcg accctcgagc
     5701 gcacgaccga gcgtcaactc gtcggcgtac tccagatcgc cgccgacggg cagcccgctc
     5761 gcgatccgcg acacggtgag accggggacg tcgagcacac gctgcaggta cagcgccgtg
     5821 gcctcgccct cgatgttggg gttggtgcac aggatcacct cggtgatccc ctccgctccg
     5881 acccgaccga ggagttcctt gatcttgagc tgatcggggc cgaccccttg gaggtggtcg
     5941 atggcgccgc cgagcacgtg gtagagcccg cggtactcac gggtgcgttc gatggcgacg
     6001 acgtcgcggg gctcctcgac cacacagagc tgatgagcgt cgcgctggtc gtccgagcag
     6061 atcgcgcaca ggtcaccctc ggccaggttg tagcaccggg tgcagaagct gaccttctcg
     6121 acggcggaac tgatggcgct caccaggcgc tgggattcgg ccttgtccac cttgagcagg
     6181 tggaacgtga tgcgctgcgc ggatttcgga ccgatgccgg gcagccgacc gaaggcgtcg
     6241 atgagctcct ggatcgagtg ggcgtagtcg gccatcaggc tccgccgaag agcccaccca
     6301 ggtcgccgag cccggccaat gggttggcct cggactgcag cgcgacgacc ttgctggtgg
     6361 catcgcggag cgcggcgagc accagatcct cgagcatggc tcggtcatcg gggtcggtgc
     6421 cgtcgggaag gacctcgggg ctgatcgtca cgctctggaa ctcgaagccg ccggtgacgg
     6481 cgatcgtgac cgcacccccg ccggactggc cctcgacgac ggtcgctccg atgcgctgct
     6541 ggctctcgag catctgcgcc tgcatgttct gcgccgcgcc gaggagcgac cccaggtcga
     6601 gacctccgag accgcccggt gccgcgtcgt caccgacgac ctccaccggg tcgttcacgt
     6661 tctcgggaag tgcgccgtcg gtcatggggt ctcctgttcg atcagttcgg caccgggaaa
     6721 tgcctgcacc agcttgtcga ccccggaggt cgagacgtcg gtcgcgtcct cgaggtcgga
     6781 cacgtcgatg tggtcgcgtt cgtcctggtc ggtgtcggct gcgacgacgg gggtcggggc
     6841 cggcgttccg gacgacgacg gcgcggacga cgaaccctcg ccgccgttgg tgaggtgcac
     6901 cgacaccggc tgcccgacgc ggacgctcag cgccgcctcg acgtcggcga gtgcggcctg
     6961 cgcccgggga aggacccggt cgggcacgtt gtcgagggcg aactggaact tgccgtcggc
     7021 ttcgccgacg aaccgaccac cgccgtacag cgacttggtc atgcctgaca gggtcgggag
     7081 caccgactcg gtcatcgccg ccgccagctg ctcgagcgtc accgtgccgc caccagcagc
     7141 cggcgcgggt gcgggtgcag ttgttggtgc agacggttcg gacaccagcg cgacgggagc
     7201 ggggggtgcc gcggaggagg gcgctggtgc aggtgccggc gtcgcggccg gggcggacgg
     7261 cgaggggtcg gcggacccgg gccgcgacgg ggcgccggga cgggtgggcg gcttgggcgg
     7321 ggacggacgc gtcggtgcgg atggcgcgtc gcccttgcga gctgcgagtt gggcgcggat
     7381 ctcggcaggg ctcgacgcag cgccggaagt cggtgccggc tccggatgag gttcgacgac
     7441 cggcgtggcg ggggcgggtg tgtccgaacc cggggacgtg gccggtgccg gcgcggcggg
     7501 agcggcaccc gccgggatgc cacgggcgag gcggcgttcg aggtcgtcga tgcgctcgac
     7561 gagggcctgc accgacgtgt cggcgctcgg cgtcgtcagg cggatcaacg cgacctcgag
     7621 cgggacccga gggtcggcgg cctggcgcat gtcgacgatg gcgagcccga gcgattcgag
     7681 cgctcgggtg atggtcgccg taccgagccg acccgccagg tcggccatct gggcctggtc
     7741 actgtcggac aggtgctcga ggtgggcgtg cacggacgcg aggaacgcgt cgcgcaggct
     7801 gaccagcagc tgctcggcga ggacccgcgg ctcgcgccct tgggccaccg cgtcggagac
     7861 cgcgatcatg acgcgggcgg tgtcgcggtc gacgagtgcc gtcatgatct cctcgaccga
     7921 ctcggcacgg tcggtgacgc cgccggcggc gaccaccatg tcgagtgccg acagggtgtc
     7981 gcgcgccgaa ccccggccct gccgcaccac gtggtcgact gcggcgtcat cgaacgtgag
     8041 gccggcgtcc tcgcgaatcc accggacgta gtcggcgagc tcggtcgccg agaggagcga
     8101 gaactcgaag tgctgtgtcc gcgagcgaat cgtgggcagc accttctggg gatcggtggt
     8161 ggccagcacg aagcacacgt gctccggcgg ctcctcgagc gtcttcagca gcgcgttgga
     8221 cgccgccggc gacaacatgt gcacctcgtc gaggatgtac accttgttgc ggcccggcga
     8281 cccgacgacg gtacggctga tcagctcccg catggcgtcg acgccgttgt tcgaagcggc
     8341 gtcgagttcg aacaggtcgt agctgttgcc gtgctcgatg ctgaggcagc tctcgcactc
     8401 gccgcacggc tcgccgtcga cgaggttcgg acagttgagc gctttcgcga ggatccgcgc
     8461 cgtggacgtc ttgccggtgc cacgggggcc gctgaacagg taggcgtgac cctcggtgcc
     8521 ctgggccacg gcgttgcgca gcgccttcac cacgtggtcc tgcccccgca cgtcggcgaa
     8581 gcggcggggc cggtagcggc ggtagagcga ttggtgggcc atcgggggcc agcctagttg
     8641 tcgccctctg acgcccagac ggggcgggcc tcggagtgcg gacccctgag agcggggtca
     8701 ggtcggcgcg gtcccgacgg acggatcgat gttgccgctc cgtatgctca cggcgtgtcg
     8761 tttcccgtcg cgcctccgcc ggccacggcc ccgagcgcgc ctcccggttg gtaccgcgac
     8821 caccacaacg agcgctggct ctggtgggac gggtggcagt ggatcacgcc gccggagccc
     8881 actccagtgt cggacgatcg cacatggttc ccccgcaccc cgacgctcca gcttcccgcc
     8941 gcagtgctgg gcatcgtgct catcgtcgtg ctgacgatcg ggacgagggt ggcggcgctg
     9001 cttcccgaga cgcccgaggc gctggtgcaa ctcgccctgt tcgccgtgag caccttcggc
     9061 atgccgctgg cggcgtggta cggatcccga cgttggggca ccggccggtt cgtcgacgat
     9121 ctgggattcc ggttccgatg gatcgatgtc ccgctcggca tcggcggagc gatcgccctc
     9181 acgatctcgt tgatggtgat caacatcgtc gcccacgtgc tgggggcgcc gagtggttcc
     9241 aacctcaccg aggtgtccga acgcggccgc gacgtcgtca cgttcgcgac gctcttcgtc
     9301 accgccggcc tcatcgctcc cctcaccgag gagctgctgt tccgcggtct catccaacgg
     9361 ggcctgtcgt cacggtggac gccctggatc gccgtcgcta tgcaggccgc cgtgttcggc
     9421 gcggcgcaca tcaccccgag cgaggggtgg ggcaacgccg acctcatcgt cagcctcgcg
     9481 gtgatgggat tcggcctcgg ggcgatcgcc agactgaccg ggcggctggg cacgagcatc
     9541 gtggcgcacg cgctcttcaa ctgcatccaa ctcgcgctgc tctggctcac gttgtcgtga
     9601 ccgcagccac cctggtcctc ctccatccgt tgccgctcga cgggtcgatc tgggccaggg
     9661 agatcagctc gctgacgccg cgctcgatcg ctcccaccct gtacgcatac ggcgacacgc
     9721 tcgacgcgtg ggccgtgagt gtcatcgagg aggccggcga cggaccgctc gtggtggtgg
     9781 gcaactccgt gggtggatcg tgcgccctcg atgtggtccg gctcgacccc ggtcgtgtcc
     9841 agctcctcgt gctcgtcggg acgaaggccg cccaccgtcc cgaaccacgc gtccgcgacg
     9901 aagccctcga gctcctggcc agagatggga tcgccgctgc ctgggatcgc tactgggctc
     9961 cgttgttcgc cgctgacgcc gatccaaccg tggtcgccgc tgcgcgttca gtcgcgctgc
    10021 gtcaaaccgt tgacgacctc gccgtcgggg tcagagcgtt ccatggtcgg ccggatcgat
    10081 ccgacctcgc ccgatcaatc gacgttccaa cgctcgtcgt gtgtggagag cacgacccgc
    10141 tctcagctac agccccggcg ttcgcaggag ggctccgacg cggtcgttgc cgcatcgtgg
    10201 agggtgccgg ccactacgtg ccggtggagc ggccggccga actcgccgcc ctgatcgcgg
    10261 ccgagttgga ttccgtggcc tggtgagacg gtcaggcgat ctgcggcaca ccctggtatc
    10321 cgctgagggc tgctgccttc cgaccctgac ccgattcacg gccctgagtt gcacaggacc
    10381 cgaccgcctc accagaacac ggaccggggc agtgtagctg gacaaagtgg cggacatgcc
    10441 agcgggacca gctacgctcg atgccggtca cctgcccagg agacgtgcga gagcggacga
    10501 atcggcacgc ctggaacgcg tgtgtggggc aactcaccgt gggttcgaat cccaccgtct
    10561 ccgcagtgtg atctctcacg acataggaac cctcgaaccc tcaggttcgg gggtttgtcg
    10621 tttctgggag cggggctttc cggtgggttg gtagttccgg gtggggtcga gggtgaggta
    10681 gcggaggagc tcgccggtcg cggcgttgac gatgcggatg tcgaggtcgg cgacgaggac
    10741 gatgacgtgg gttc
//
LOCUS       DAOSOA010000085        10739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6127_length_10739_cov_5.944871, whole genome shotgun sequence.
ACCESSION   DAOSOA010000085 DAOSOA010000000
VERSION     DAOSOA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6127_length_10739_cov_5.944871"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKY13_07315"
     CDS             <1..251
                     /locus_tag="PKY13_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79778.1"
                     /translation="LHLVTVNERINRLGDDFGPDAAAFNAKLREIAARRLNTHVIDWN
                     QIVADHVADGIISEDSVHPTPAGVKLLADAYVGALRDC"
     gene            complement(304..1770)
                     /locus_tag="PKY13_07320"
     CDS             complement(304..1770)
                     /locus_tag="PKY13_07320"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT79779.1"
                     /translation="MAAQAGWYVDPRDARQYRYWDGERWSQSTQPIPEPTDTAGANDS
                     NGVATGSLSSGGAKDPVGAAAFDQAAEQTPASSDGVLADFMWTSGFGETPDDPAANAG
                     PTMFTPGDTGPAAPRPPAPPAPPAPAASAPAPSAPPTPAGPFGSTAESTSPAFPPPPA
                     APTSAFGAPTDLGTTSPSAPVGGGSLFSAPPSGAAPQFDPTPSATPTDAGGSLFAPID
                     PSLQPIFGSGDHADPAFGGAAPKRSRKGKADKAAPEPPAPAPVSLSAGGPSAFRMPEP
                     DPFVPRPDISVEPPVGPADAAGAVKIKMDRKPKSPKTKGAPKQKAQKGKDESKSKLPV
                     LLGALAVVVLGAGAFVLLSGGDDEVDTGADVAGTTVEKASTTVVAPTTTAAVVATTAA
                     ASETTAAATPTTVAAADPAAIAALQSAFDAEATRACDVIKADPGLFTENVIQYQDAWA
                     AIPKTYADLQKSVNDCSFQARDEALKRVAEAEGATTGG"
     gene            1915..3669
                     /locus_tag="PKY13_07325"
     CDS             1915..3669
                     /locus_tag="PKY13_07325"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT79780.1"
                     /translation="MAEHPADRSLDVPVVDDPSDLTPAWLTHALRSAGHDATVHAVTV
                     EPVGTGQMGSSFRLTLDVTEQSPTPSGSIPRTLVAKLPTSDVEARVAIASSHRTEVTF
                     YRDVASTIAARTPQCHLALVSEDATCFTLLLEDLAPAAQGDQLAGCTTEQLVTAAHNL
                     AGLHGPRWCDPDIVALRGPLDAADPDTAAFLGEVLAGAVAPFAERMGDAVSPADVALL
                     HDCAAAMPHFLVGRPERFSLMHGDYRLDNLMFHPDGTVAAVDWQTLGVGLAGRDLAYL
                     IATSLEPDARREAEARCDRRVPRPARRADRRCRHGARRCGHHVRRLPVRTPARAAHHR
                     PGGRVRLVDDPRRRDVRDDDGPRVRRHPRPQHALAHQLTGSVPREAHSSRSFAGQNEP
                     PARTPRVLGMTTTEPERIDCTRVGTEFFATAPCRVRVREVVPAPPLRVFDVFADADSW
                     SRWAMPITGVEWTSPWPLEVGSTRTVRMRGGMVGWEEFIAWEPPRRMAFRFNESVAGG
                     PIAFAEDYHLTDLGNGSTRIEWIMAMELSGVSGKLTPVIRSAMQVGNRLMLRKLRNYI
                     ASNPSLTDPSGAWAQPAD"
     gene            3757..4131
                     /locus_tag="PKY13_07330"
     CDS             3757..4131
                     /locus_tag="PKY13_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79781.1"
                     /translation="MTTDSNPSAGDTPLSSVTNTIDDEHGTGQFGVLEHAELRCFTCR
                     ETFPASTVPADRLTRLEGASDPSDMVVVIPVTCPHCSTSGSLVLNYGAEASVDEADAL
                     DAMERRPEVGTPGTNPTPGIQH"
     gene            complement(4128..4955)
                     /locus_tag="PKY13_07335"
     CDS             complement(4128..4955)
                     /locus_tag="PKY13_07335"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOT79782.1"
                     /translation="MTNDDGVGTTGITTLARALRDDGHDVLVVAPKDERSGAGTALGR
                     VVGGDVVSWHRTDADGVEVFALDAPPAMCVLAAMEGIAGARPDLVVSGINHGPNLGFA
                     VLHSGTVGAALTAHIAGIPALAVSVGQPATGVHGVASAADDPIWDAAAALAVALVGLA
                     DEWSGQWVPNVNVPSAWDGGPVRCCRLARLGAIGLAVNGDDSAVVQLRSHAELDEPEA
                     AVDGWPDDLALNAAGHATMTPLTGVAMRSVETDAIEALLSRLRGSIGSAAPAGYRPR"
     gene            complement(4981..5928)
                     /locus_tag="PKY13_07340"
     CDS             complement(4981..5928)
                     /locus_tag="PKY13_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT79783.1"
                     /translation="MRPTAPATGRVAVFSVAPILVITVEDDRSSATHVNVGGQGPWVA
                     RAIATLGGTPVLCAPFGGDTGRTARLLLDDSGFELSATTTASPSGIYVERRDERGTRH
                     CLYEVPAAPLGPRDLDDVYEAALRHGIETGTCVVTGSPFPDHLDPAVVSRLCTDLHAA
                     GVVVVADLSGEHLDAALDAGVDWLKVADDELTRDERCSDGEAEVWCWLDGVVEAGKVR
                     VAAVASSSTGPTLARTPRHRITAQGPHLEVVDTRGSGDAMCAALALAHGNDLPPADAI
                     RIGLAAGAANATRRSTAAPGIEAVLSLQSTVELAVQRVA"
     gene            complement(5925..6413)
                     /locus_tag="PKY13_07345"
     CDS             complement(5925..6413)
                     /locus_tag="PKY13_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79784.1"
                     /translation="MSVHRHDDVVTTPTGTARSTERSSRFSPGQVITGILGVVLAVIG
                     IVAVTRSGIDGSLNQPVTEIVGITHSSYVGLFELFVGLVLLLGASSAGYRGAAGFAGA
                     LLIVGGVVLAAGNLRVLLEVGAKQSTGWMGIVLGAIAISAAMLPSLVRTSRVVEAPVV
                     TE"
     gene            complement(6509..6868)
                     /locus_tag="PKY13_07350"
     CDS             complement(6509..6868)
                     /locus_tag="PKY13_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79785.1"
                     /translation="MTTTYRRLPSSHPTRGAATAAGAPVGRLRRGVAAAVLLAVPAFA
                     VACDDGGSPDGTTPTEEVNSDSTTSGVAPTSDPVNVPTSVSGGPAGQGSVPEQPGGTA
                     QPMDQGGMNTTPAPDAG"
     gene            7121..8104
                     /locus_tag="PKY13_07355"
     CDS             7121..8104
                     /locus_tag="PKY13_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOT79786.1"
                     /translation="MDAITLLKDDHRSLERLFKRYEQAGDRAFVEKRALVDRIIEELS
                     RHAAIEEQLFYPVARATVPGTDDIALESLEEHHIVKWVLSELERMDPTDERFDAKVTV
                     LIENVRHHVEEEESEFFPKVRDELGRNALGDLGDTMAKFKEVAPTHPHPTSPDTPPGN
                     LVTGAAAGVVDHVTDTISGVAQGTVTALQDLIALVLRRKRPSTSPTGSTVARKQAKKV
                     RNEVADLTDSAIDAAKAAQSSAERTVKAAKTGAERTVKTAKTGAKRTATTARNSADRT
                     TTTARKGAKATRSAAKATATSTARTATTAAKRTSSTARTGAKRTASAAKRG"
     gene            8106..8729
                     /locus_tag="PKY13_07360"
     CDS             8106..8729
                     /locus_tag="PKY13_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT79787.1"
                     /translation="MAGLTAFALNCTLTPSSAGASSTGKLLEEVLLELSTHGVTSQGS
                     VRLADFTISPGVSSDEGGDDEWPDIRTRILDADIFVLGTPIWMGHACSNAQRVLERLD
                     AFLGETDDQGRMPAFGKVAIVAVVGNEDGAHHVAAELFQGLDDVGFTIPANGMTYWVG
                     EAMHTTDYQDLPEGSAKTDSATKLAASNAAHLAALLSGANYPGPGSD"
     gene            complement(8763..9797)
                     /locus_tag="PKY13_07365"
     CDS             complement(8763..9797)
                     /locus_tag="PKY13_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HOT79788.1"
                     /translation="MDDAPALPAPRGPMSQHLFEYLRLGSDVLPSLADLPWCEDPLGD
                     DDLQLTLYCCYELHYRGFSDVSDDVEWDPEVLRFRRELEGRFEDALRNCIQPSPRAAL
                     TPADELAAIIDTGAGHPSPSRFMAEEGTIEQMREFAIHRSMYQLKEADAHTWGIPRYA
                     GPSRSAMIEIQMDEYGNGRPGEAHAELFADTMRALGLDDSYGAYLERVGAPTLATMNL
                     VSWLGLHRRLLPALLGHLAVFEMTSVVPMGRYAAACDRLGLGPEARRFYDVHVEADAH
                     HGPLAATHLVGDYVSEHPESRQLVMWGAWAVMEVERRLAAHLMACWERGASSLRTEVE
                     PTGRPHLAAA"
     gene            9901..10416
                     /locus_tag="PKY13_07370"
     CDS             9901..10416
                     /locus_tag="PKY13_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOT79789.1"
                     /translation="MDATDLLVDAVERIRELMHQHGDLDTAVTQRQPAKGSNSIGWLL
                     WHTARVQDAQVAPLIGEPQLWVAEDFARRLGFDPDENDTGYAHTVAQAAAVLIDDLSV
                     LLEYHDAVTGRTIEWLRSCEDADLDRVIDPRFDPPVTVGVRLVSIVADSLEHLGQAGY
                     VRGLLGAPPPG"
     gene            10487..>10739
                     /locus_tag="PKY13_07375"
     CDS             10487..>10739
                     /locus_tag="PKY13_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79790.1"
                     /translation="MFDTTVLTELTAKIAGLDEHLYADDDLLAGVKAIEEARRHLDAA
                     EGALLGELDARGTTDVVSGHRTANWLAHETGIAPAVAKSR"
BASE COUNT         1667 a         3896 c         3680 g         1496 t
ORIGIN      
        1 gcctgcacct cgtgaccgtc aacgagcgca tcaaccgcct gggcgacgac ttcgggcccg
       61 acgccgccgc cttcaacgcc aagctgcgcg agatcgcagc gagacgcctc aacacccatg
      121 tgatcgactg gaaccagatc gtcgccgacc acgtcgccga cggcatcatc tccgaggact
      181 cggtgcaccc caccccggcc ggggtgaagc tcctcgccga cgcctacgtc ggcgcgttgc
      241 gcgactgttg acccgcagcg ttgacccgca aagtcgttga ccgactggcg ctgcgccgga
      301 cgatcagccg ccggtggtcg cgccctcggc ctcggcgacc cgcttgaggg cctcgtcgcg
      361 ggcctggaac gagcagtcgt tcacgctctt ctgcagatcc gcgtaggtct tcggaatcgc
      421 ggcccacgcg tcctggtact ggatgacgtt ctcggtgaac agcccgggat cggctttgat
      481 gacatcgcag gcgcgggtcg cctcggcgtc gaacgccgat tgaagagcgg cgatcgcagc
      541 ggggtcggcc gcagcgacgg tggtgggcgt cgcagcagca gtggtctcgc tcgcggctgc
      601 cgtggtggcg acgaccgcgg cggtcgtcgt gggcgccacc accgtcgtgg acgccttctc
      661 gacggtcgtg cccgccacgt cggcgccggt gtcgacctcg tcgtcgccac cggacagcag
      721 gacgaacgcc ccggcgccga gcaccacaac ggcgagcgca ccgagcagca ccgggagctt
      781 cgacttcgac tcgtccttgc ccttctgcgc cttctgcttg ggcgcaccct tggtcttggg
      841 cgacttgggc ttgcggtcca tcttgatctt gaccgcgccg gctgcatcgg cagggccgac
      901 gggcggctcg acggagatgt cgggccgggg gacgaacgga tcgggctccg gcatgcggaa
      961 cgccgacggc ccgccggcac tcagcgagac cggggcgggt gccggcggct cgggcgccgc
     1021 cttgtcggcc ttgcccttcc ggctgcgctt gggagcggca ccgccgaacg ccgggtcggc
     1081 gtgatcgccg gacccgaaga tcggttggag cgacgggtcg atcggcgcga acagcgatcc
     1141 tccggcatcg gtcggcgtcg cggagggggt cgggtcgaac tgcggtgcgg caccgctggg
     1201 cggagcgctg aacagcgatc ccccgccgac gggtgcggac ggtgaggtcg tcccgaggtc
     1261 ggtgggcgca ccgaacgcgg aggtcggcgc ggccggaggc ggcgggaacg ccggcgaggt
     1321 cgactcggcg gtgctcccga acggcccggc gggggttggc ggggccgagg gcgcgggtgc
     1381 cgatgcggcc ggggccggcg gagcgggcgg agcgggcggc cggggcgctg cgggtcccgt
     1441 gtcaccgggg gtgaacatcg tcgggccggc gttcgcagct ggatcatcgg gcgtctcacc
     1501 gaagccggac gtccacatga agtcggccag taccccgtcg ctggaggcag gagtctgctc
     1561 agcggcctgg tcgaacgctg cggcgcccac cgggtccttc gcgccgccgg aactcagcga
     1621 tccggtggcc acgccgttcg agtcgttcgc gccggcggtg tcggtcggct ccgggatggg
     1681 ctgggtggac tgggaccagc gttcaccgtc ccagtatcgg tactggcgcg cgtccctcgg
     1741 atcgacgtac caaccggctt gcgctgccat gttcctaccc ccgaagcccg attgcccatt
     1801 gctgccgcgg cgaagggtat cggcaaccac cccgcgaagt cacctcccca ttctgagcga
     1861 gatcgcagct cggtttcccg gcccggcaac tggacacgtg tctagattgg ggcgatggca
     1921 gaacaccccg ccgaccgctc cctcgacgtg cccgtcgtcg acgacccgag cgacctcacg
     1981 ccggcctggc tcacccacgc cctccgatca gcgggccacg acgccaccgt gcacgctgtc
     2041 actgtcgagc cggtcgggac cggtcagatg gggtccagct tccggttgac gctcgacgtg
     2101 accgagcagt cgcccacccc gtcgggttcg attccacgaa ccctcgtcgc caagctgccg
     2161 acctccgacg tcgaggcccg ggtcgccatt gcgtccagcc atcgcaccga ggtgacgttc
     2221 taccgcgacg tcgcatcgac gatcgccgcc cgcacgccgc agtgccacct cgccctcgtg
     2281 tccgaggacg ccacctgctt cacgctcctg ctcgaggatc tcgcgcccgc cgcgcagggc
     2341 gaccagctcg cgggctgcac caccgagcaa ctggtgaccg ccgcccacaa cctggccggg
     2401 ctgcacggac cccgctggtg cgaccccgac atcgtggccc ttcggggtcc actcgatgcc
     2461 gccgatcccg acaccgcggc gttcctcggc gaggtcctcg ccggggcggt cgcgccgttc
     2521 gccgagcgga tgggcgacgc cgtgtcgccc gccgacgtcg ctttgctcca cgactgcgcc
     2581 gccgccatgc cgcacttcct cgtcggccgg ccggagcggt tctcgctgat gcacggcgac
     2641 taccggctcg acaacctgat gttccacccc gacgggacgg tcgcggcagt cgactggcag
     2701 acgctcggcg tcggccttgc cggccgggac ctcgcctatc tcatcgcgac cagcctcgaa
     2761 cccgacgcgc ggcgggaagc ggaagcacgc tgcgatcgcc gcgtaccacg accggctcgt
     2821 cgggctgacc gccggtgccg gcacggagcc cgtcgatgcg gccaccacgt tcgacgacta
     2881 ccggtacgga ctcctgcaag ggccgctcat catcgtcctg ggggccgcgt tcggctcgtc
     2941 gacgacccgc ggcgacgcga tgttcgcgac gatgacggcc cgcgtgtgcg ccgccatccg
     3001 cgaccacaac acgctctcgc tcatcagctg accggttctg tcccgcgaga ggcgcacagt
     3061 tcgcgctcct tcgcgggaca gaacgagcca ccggcgcgca cgcctagggt tctgggcatg
     3121 acgacgacgg aaccggaacg catcgactgc acacgggtcg gcacggagtt cttcgccacg
     3181 gcgccctgtc gcgtccgggt gcgcgaggtc gtccccgctc ccccgctgcg ggtgttcgac
     3241 gtcttcgccg acgccgactc ctggagccgg tgggcgatgc ccatcaccgg ggtcgagtgg
     3301 acgtcgccgt ggccgctcga ggtcgggtcg acccggaccg ttcgcatgcg cggcggcatg
     3361 gtcgggtggg aggagttcat cgcgtgggaa ccgccccggc gcatggcctt ccgattcaac
     3421 gagagcgtgg ccggcggccc catcgcattc gccgaggact accacctcac cgacctcggc
     3481 aacggcagca cccggatcga gtggatcatg gcgatggagc tgtcgggcgt gtcgggcaag
     3541 ctgaccccgg tcatccgctc ggccatgcag gtcggcaacc gcctcatgct ccgaaagctg
     3601 cgcaactaca tcgcctcgaa cccatccctc accgacccct ccggggcgtg ggcgcagcct
     3661 gccgactgac gcacggggca agccacacct cccctcgaag cggggatcgc cgccgacggt
     3721 tcgtctcgcg gtccgacacc gggggtagtg cccggcatga ccaccgactc gaacccgtcc
     3781 gccggcgaca cgccgctgag ctccgtcacg aacaccatcg atgacgagca cggcaccggc
     3841 cagttcggtg tgctggagca cgccgaactc cgatgcttca cgtgtcgcga gacctttccg
     3901 gcctcgacgg tccccgccga ccggttgacc cgcctcgaag gcgcctcgga cccatccgac
     3961 atggtcgtcg tgatcccagt cacgtgtccg cactgctcga cgagcggttc actcgtgctc
     4021 aactacggcg ccgaggcgag cgtggacgag gccgatgccc tcgacgccat ggagcgccgc
     4081 cccgaggtgg gcacgccggg caccaacccc actccgggca tccagcacta acgcggccgg
     4141 taccccgccg gcgcggccga gccgatcgag ccgcgcaacc tcgacaacag cgcctcgatc
     4201 gcgtccgtct cgaccgaacg catcgcgaca ccggtcaggg gcgtcatcgt cgcgtgtccc
     4261 gcggcgttga gcgcgaggtc gtcgggccaa ccgtccacgg ccgcctcggg ctcgtcgagt
     4321 tcggcgtgac tccggagctg caccaccgcg ctgtcgtcgc cgttgaccgc gaggccgatg
     4381 gccccgagtc gggccagtcg gcaacagcgg accggcccgc cgtcccacgc cgacggcacg
     4441 ttcacgttgg gcacccactg accgctccac tcgtcggcca acccgacgag ggccacggcg
     4501 agcgcagcag cggcatccca gatcggatcg tcggccgcgc tcgcgacgcc gtgcaccccc
     4561 gtcgccggtt gccccacgct cacggcgagc gccgggatcc cggcgatgtg ggccgtgagc
     4621 gccgcgccca ccgtgccgga gtgcagcacc gcgaacccga ggttcggacc gtgattgata
     4681 cccgacacga cgagatcggg gcgcgccccg gcgatccctt ccatggcggc gagcacacac
     4741 atggccggcg gagcgtcgag cgcgaacacc tcgacgccgt ccgcgtcggt gcggtgccac
     4801 gacacgacgt cgccgccgac cacccgcccg agggcggtgc cggcgcccga acgctcgtcc
     4861 ttcggagcca cgacgaggac gtcgtgaccg tcgtcgcgca acgcccgcgc cagcgtcgtg
     4921 atcccggtcg tgcccacgcc gtcgtcgttc gtcacgcaga tccgcatcgg cccgaccgcc
     4981 tcacgccacc cgctgcacgg ccagctcgac ggtcgactgg aggctgagca ccgcctcgat
     5041 gcccggcgcc gcggtggacc gtcgggtggc gttcgcagcg ccggccgcga gaccgatccg
     5101 gatggcgtcc gccggcggaa ggtcgttgcc gtgggcgagg gcgagcgccg cgcacatggc
     5161 gtcgcccgac ccgcgggtgt cgacgacctc gagatgggga ccctgtgccg tgatgcgatg
     5221 ccggggcgtt cgcgccagcg tgggtccggt cgagctcgag gcgaccgcgg cgacacgcac
     5281 cttgccggcc tcgaccacac cgtccagcca acaccacacc tccgcctcgc cgtcgctgca
     5341 tcgctcgtcg cgagtcagtt cgtcgtcggc caccttgagc caatcgaccc cggcatcgag
     5401 ggcagcgtcg aggtgctcgc cgctcaggtc ggccacgacg acgacaccgg ccgcgtgcag
     5461 gtccgtgcac aggcgactga cgacggccgg gtcgaggtgg tcggggaacg gtgacccggt
     5521 gacaacgcag gtgcccgtct cgatgccgtg acgcagcgcg gcctcgtaga cgtcgtcgag
     5581 gtcccggggt ccgagcggag cggccggcac ctcgtacaga caatgtcggg taccgcgctc
     5641 gtcgcggcgc tcgacgtaga tcccgctcgg cgaggccgtc gtcgtggccg acagctcgaa
     5701 accggagtcg tccaacagca ggcgcgccgt gcgtccggtg tcgccaccga agggagcgca
     5761 gaggaccgga gtgccgccca acgtggcgat agcccgggca acccacgggc cctggccccc
     5821 cacgttgacg tgggtggccg acgaccggtc gtcctcgacg gtgatcacga gaatgggggc
     5881 gacgctgaag accgcgaccc ggcccgtcgc cggcgcggtg ggtctcattc ggtgaccacg
     5941 ggggcctcga cgacgcgtga ggtgcgcacg aggctcggca gcatggccgc ggagatcgcg
     6001 atcgcgccga gcacgatgcc catccacccc gtcgactgct tcgccccgac ctcgagcagc
     6061 acgcgcaggt tgccggcggc gagcacaacg ccgccgacga tcagcagcgc gccagcgaag
     6121 ccggccgctc cccggtagcc ggcgctcgag gcaccgagca ggaggacgag accgacgaag
     6181 agctcgaaca gtccgacata ggagctgtgg gtgatcccga cgatctcggt gaccggctgg
     6241 ttcaacgaac cgtcgatgcc cgaacgggtg acggccacga tgccgatcac ggcgagcacg
     6301 accccgagga tcccggtgat cacctggcca gggctgaagc gcgacgaccg ttcggtcgat
     6361 cgggcggtgc cggtgggtgt ggtgacgacg tcgtcgtggc ggtggacgga catggcggcc
     6421 tcctcgggtc agggtgcctg ccgatgcccg agcgacgccc gcgacaaaca ccccgttgga
     6481 gggacccgac ggggaggcgg cgaggagatc agccggcgtc gggtgccggc gtcgtgttca
     6541 taccgccctg gtccatcggt tgcgccgtgc ccccgggttg ctcgggcacc gagccctgtc
     6601 ccgcaggtcc gccgctgacg ctcgtcggaa cgttcacggg atcgctcgtc ggggccacac
     6661 ccgacgtcgt cgagtcggag ttgacctcct cggtcggggt ggtgccgtcg ggcgaaccgc
     6721 cgtcgtcgca ggcgacagcg aacgcgggga cggccagcag caccgcggcg gcgacacccc
     6781 gccggagccg accgaccggg gcgcccgcgg ctgtagctgc acctctcgtc gggtgactcg
     6841 aaggtaggcg tcggtaggtg gtcgtcactg ggtgctcctg aggtctccgg cccgcagtag
     6901 gtgcgtttgc cggtcggccc gacctacccg atcacggcat ctgcgagtca tccgccgctg
     6961 actcgttcct cagaacccat caggaggtca ccgcgaacag cgcaggatcg gtcatcgctg
     7021 gatcggtcat ggcggatgct tcccgatcga cccacgcccc aaacgggggg tcttcgctcg
     7081 cgatcgtttg ggcgtcgaag ccgacgggta gcgcggaagt atggacgcaa tcacccttct
     7141 caaggacgac caccggtcgt tggaacgact cttcaagcgc tacgagcagg ctggcgaccg
     7201 cgccttcgtc gagaagcggg ccctcgtgga ccgcatcatc gaagaactct cccgccacgc
     7261 cgcgatcgag gagcagctct tctacccggt cgcccgggcg accgtgcccg gcaccgacga
     7321 catcgctctc gagagcctcg aagagcatca catcgtcaag tgggtgctga gcgagctcga
     7381 gcggatggac ccgaccgacg aacgcttcga cgccaaggtc accgtgctca tcgagaacgt
     7441 tcgccaccac gtcgaggagg aggagagcga gttcttcccg aaggtgcgcg acgagctcgg
     7501 gcggaacgcg ctcggcgatc tcggcgacac catggcgaag ttcaaggagg tcgctccgac
     7561 ccacccgcac cccacctcgc ccgacacccc tccgggcaac ctggtcaccg gcgctgccgc
     7621 cggcgtcgtc gaccacgtca ccgacaccat cagcggcgtc gcccagggga ccgtgacggc
     7681 gctacaggac ctcatcgctc tggtcctgcg ccgcaagcgc ccgtcgacgt cccccacggg
     7741 ctcgaccgtc gcccgcaagc aggcgaagaa ggttcgcaac gaagttgctg acctcaccga
     7801 ctcggccatc gacgccgcca aggccgccca gagcagcgcc gagcgcacgg tcaaggcagc
     7861 gaagaccggc gcggaacgca ccgtgaagac tgcgaagacc ggcgccaagc gcacggcaac
     7921 gacggcccgc aacagtgccg accggacgac gacgacggcc cgcaagggag ccaaggcgac
     7981 acggtcggcg gccaaggcga cggccacgag cacggcccgt accgccacca ccgcggccaa
     8041 gcggacctcg agcacggcac gcaccggggc gaagcgcacc gcgtccgccg ccaagcgcgg
     8101 ctgacgtggc aggcctcacg gcgttcgccc tgaactgcac gctcaccccg agcagcgccg
     8161 gtgcatcgag caccggcaag ctgctcgagg aggtgctcct cgaactgtcg acgcacggcg
     8221 tcacgagcca gggttcggta cgactggccg acttcacgat ctcgccgggt gtcagttccg
     8281 acgagggcgg cgacgacgaa tggcccgaca tccgcacccg gatcctcgat gccgacatct
     8341 tcgtcctcgg caccccgatc tggatgggcc acgcctgcag caacgcccaa cgcgtgctcg
     8401 aacggctcga cgcgttcctc ggcgagaccg acgaccaggg tcgtatgccc gcgttcggga
     8461 aggtcgcgat cgttgccgtc gtcggcaacg aggacggtgc acaccacgtc gcagcggagc
     8521 tgttccaagg cctcgacgac gtcggcttca cgatcccggc caatggcatg acgtactggg
     8581 tcggcgaggc aatgcacacg accgactacc aggacctgcc cgagggcagt gccaagaccg
     8641 actcggcgac gaagctcgca gcgtcgaacg cggcacacct ggcggcattg ctcagcggcg
     8701 ccaactaccc ggggccggga tcggactgac ccgacctcgc cgccttcggg ccggtcagcc
     8761 gttcatgccg cggcgagatg ggggcgcccc gtgggctcga cctccgtccg cagggacgac
     8821 gccccacgct cccagcacgc catcaggtga gcggcgaggc gccgctcgac ctccatcacg
     8881 gcccacgcac cccacatgac gagttggcgg gactcgggat gctcactcac gtagtcgccg
     8941 acgaggtgcg ttgccgccag cgggccgtgg tgggcgtcgg cctcgacgtg cacgtcgtag
     9001 aagcgtcgcg cttcgggtcc gaggccgagg cgatcacacg ccgcggcgta ccgacccatc
     9061 gggaccaccg acgtcatctc gaacacggcc aggtgcccga gcagtgccgg caggagtcgc
     9121 cggtgcaacc cgagccacga caccaggttc atggtggcga gcgtcggcgc gccgacccgt
     9181 tcgaggtagg cgccgtagct gtcgtcgagg ccgagggccc gcatcgtgtc ggcgaacagc
     9241 tccgcgtggg cctcgccggg acgaccgttg ccgtactcgt ccatctggat ctcgatcatc
     9301 gccgagcgcg aggggccggc gtaacgaggg atgccccagg tgtgggcgtc ggcttccttc
     9361 agttggtaca tcgagcgatg gatcgcgaac tcgcgcatct gctcgatcgt gccctcctcc
     9421 gccatgaacc gcgacggcga cggatggccc gcgccggtgt cgatgatcgc ggccagctcg
     9481 tcggccgggg tgagcgccgc acgtgggctc ggctggatgc agttccggag cgcgtcctcg
     9541 aaacgaccct cgagctcacg ccggaaccgc aacacctccg ggtcccactc gacgtcatcg
     9601 gagacgtcgg agaacccgcg gtagtgcagc tcgtagcagc agtacagcgt gagctgcagg
     9661 tcgtcatcgc cgagggggtc ctcgcaccac ggcagatcgg cgagtgacgg cagaacgtcg
     9721 ctgccgagcc gcaggtactc gaagaggtgc tgcgacatcg gtcctcgggg ggcgggaagt
     9781 gcaggtgcgt catccatgat gcttcctgat tccccgtcag acccgctccg cagccccctc
     9841 gtcggaggga tttcggccgt gacccgaggc gagctgccgg ccacggggta ggcagtagcc
     9901 atggacgcga ccgacctcct cgtggacgcc gtcgagcgga tccgcgagct catgcaccag
     9961 cacggcgacc tcgacaccgc ggtcacacaa cgccagccgg cgaagggatc caactccatc
    10021 ggctggctgc tctggcacac ggcccgggtg caggacgccc aggtcgctcc gctgatcggc
    10081 gagccgcagc tgtgggtcgc agaggacttc gcccgtcgac tcggcttcga ccccgacgag
    10141 aacgacaccg gctacgccca caccgtggcc caggcggccg ccgtgttgat cgacgacctg
    10201 tcggtcctgc tcgagtatca cgacgccgtc accggtcgaa cgatcgagtg gctccggtcg
    10261 tgtgaggacg ctgatctgga tcgggtcatc gacccgcgct tcgacccgcc ggtgaccgtc
    10321 ggggtgcgcc tcgtcagcat cgtcgccgac agcctggaac acctcggcca ggccggttac
    10381 gtgcgcggtc tgctcggcgc tccaccgcct gggtgattgc acccgatcga cacgaaacgc
    10441 cctgatctgc agttgactgt cagacccccc gagtacactc gaacacatgt tcgacacaac
    10501 agtgctgacc gagctgacgg cgaagatcgc cggcctcgac gagcacctct acgcggacga
    10561 cgatctgttg gctggggtga aagcgatcga ggaagcccgc cgtcatctcg acgctgcgga
    10621 aggcgcgttg ttgggtgagc tcgacgcccg tggcaccacc gacgtggtgt cgggtcatcg
    10681 caccgcgaac tggttggccc acgagaccgg catcgcccct gcggttgcga agtcgcggg
//
LOCUS       DAOSOA010000086        10678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6183_length_10678_cov_6.690012, whole genome shotgun sequence.
ACCESSION   DAOSOA010000086 DAOSOA010000000
VERSION     DAOSOA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6183_length_10678_cov_6.690012"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1581
                     /gene="urtA"
                     /locus_tag="PKY13_07380"
     CDS             325..1581
                     /gene="urtA"
                     /locus_tag="PKY13_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HOT79791.1"
                     /translation="MSHGRKYKWAIAMMAASALLLSACGDDSADDSASGSSGGDSSGT
                     CVDGDSVKLGFLNSTSGAMAISEQTVRDSLMLAAKEINAGGGIMDKKIEFVEEDGQSE
                     PTVFAEKINKLLTEDQVAAVFGGWTSASRKAMLPVVEGANGLLFYPVQYEGLEASKNI
                     YYTGATTNQQIIPAMDFLKTKDVKTLFLAGSDYVFPRTANKIIKQYAKELGIEIVGEE
                     YVPLDSDDWTTQVAKIAAAKPDFVFNTINGSSNVGFIKAYYEAGLGPDNSPIISVSIA
                     EAEAPAMGEDVTGQFAAWNYCQSVESPENTKFIEAFKAEYGEDRPTSDPMEAAYTSLY
                     LYKGMVEKAKSFCVDAINEASDGVSFDAPEGKVTVNGENHHIAKTGLIGQINSDNQFD
                     IVWSSDAPIEPDPFLEGYSWWDPNAG"
     gene            1701..2585
                     /gene="urtB"
                     /locus_tag="PKY13_07385"
     CDS             1701..2585
                     /gene="urtB"
                     /locus_tag="PKY13_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HOT79792.1"
                     /translation="MEAFTTPFLTGTATGAILLLAALGLSLTFGQMGVINMAHGEFLM
                     AGAYAAYATQQVITGAGISIVFAVPVAFLLAGLLGLILEASVIQWMYKRPLDTLLVTV
                     GVSLVLQQAAKDVFGAQGDPVKPPSWLRGGLSVFGYSWPYRQAFTIVLALAVLAGLNA
                     VLKYSSFGRQIRATVQNRELAETMGVSTRKVDRITFFLGSGMAGVGGVAIALISGTNP
                     SLGTTYIIPAFLVVVAGGVGQLKGTLIAAWVVGVVTAFLTDWISGSMAQVISFALVVV
                     FLQLRPQGLFTVRTRSLA"
     gene            2582..3766
                     /gene="urtC"
                     /locus_tag="PKY13_07390"
     CDS             2582..3766
                     /gene="urtC"
                     /locus_tag="PKY13_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HOT79793.1"
                     /translation="MTTTIDTTEASDTVEATPPVGTTAGRFSPAYLFKTYGGLVGIAL
                     VAIVLLVWAPNSLTAFRLGNLGKYCAWAIAAVGIGLAWGRGGMLVMGQGVFFGLGGYA
                     MAMHMKLEAAGPGKVPDFMVLYGDATMPSWWEPFRSGAFTIFAIFAIPALISFILGYA
                     ILKRRVKGAYFAILTQALAVAFATLLVATIKQTGGFNGLNTFTTVFGQNMYDPDVKKN
                     LYMVAAAMLIVCLLISWQLYRSRYGELLVATRDAEERIRFLGYDPANIKLVAFVIAAV
                     MASLGGALFAPLAGIISPSNVDATASIMLLAGVALGGRASLFGAALGAIAVGYGQSTL
                     SEQFPTQWTYFQGALFIVVMLFLPGGIASLWRKVSAALPKRSPKEATVDVTVPTQTEA
                     VA"
     gene            3763..4530
                     /gene="urtD"
                     /locus_tag="PKY13_07395"
     CDS             3763..4530
                     /gene="urtD"
                     /locus_tag="PKY13_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HOT79794.1"
                     /translation="MTASFLGEDYLEVRGLTVDFDGFKAISGVDVTFLQGRLHFLIGP
                     NGAGKTTLVDALTGLVHGTGTAGFRGQDLLSMKSHKIVRLGVGRTFQTATVFEQLTVL
                     QNLDIAAGLHRKAAGLLLSRRKVPESVEQALETIGLTALANRPAGILAHGQKQWLEVG
                     MLLVQDAKVMFLDETVAGMSSEEREETGELLKRIVGDRTIVVIEHDMEFMRRYADFVT
                     VMHGGKVLAEGTVAEVQADTKVQEVYLGTVTEVAADA"
     gene            4523..5215
                     /locus_tag="PKY13_07400"
     CDS             4523..5215
                     /locus_tag="PKY13_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT79795.1"
                     /translation="MPEMMLEIDGVTAGYGRSMVLFDVSASIPVGGGAAIMGHNGAGK
                     TTLLRVAVGLIPVKSGKVLLDGEDVTNLAPNQRVKRGLGYVPQGQLSFTQMTTMENLQ
                     LITTVKSEIDEVLDTFPALTELLKRPAGLLSGGQRQQLAIARTLLTKPRLLILDEPTE
                     GIQPNVVADIEQVIVDLTQRGDLTVLLVEQHVGFALRSTDTYYVLESGRITGSGAGGE
                     GALDEVRLAMAV"
     gene            5257..5721
                     /locus_tag="PKY13_07405"
     CDS             5257..5721
                     /locus_tag="PKY13_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOT79796.1"
                     /translation="MTYEVDFKDVSTVGLESSPVVDALAGLRANEARYFKNKFDHTFV
                     TEPASKAKTTITYVHRILEDERNIEIESPVLEASEFEVDGTRWAYVFYESGLAINVMY
                     AIADGGKRAVGFKLSDGMDVPEELEAKFKFARQKSKLAGTIRGSYFVIKGTY"
     gene            5768..6577
                     /locus_tag="PKY13_07410"
     CDS             5768..6577
                     /locus_tag="PKY13_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT79797.1"
                     /translation="MATGSIRRRVAAIIAGACLVATACGPPPSAPDPAPLRAGQAPLR
                     VMVVGDSFGLSVGLGLTHYGESSGQIEVRNSAFIGCPFGRGGRNNGIGLDRDWLPECR
                     SQDERLATVSAEFRPDVVLLAGGMWDVADRLLPGSGRWTRIGEPAYDWYLALEFQHLS
                     RLLGTAGAKVVWTTAPRWNPRYQPDQFMGRPPYAEAGAGRSDRYNQVLNAAVGGMPGV
                     HVLDLAGWMRYFPGGEFSPDLRTDGVHLTPQSTDVVANEFLGPWVVAIGRS"
     gene            6643..7713
                     /locus_tag="PKY13_07415"
     CDS             6643..7713
                     /locus_tag="PKY13_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOT79798.1"
                     /translation="MRTYWSSDHVHHDPGRLPQPAGGTANYYSEVAARGPAVLDAIDA
                     AGFGTIVDLAGTIDADVVPPAALEVHDPAMVEFLRTAFDRIAAEEQWPVDDTDGSERV
                     VIAETFGRTDDPSLERSTSPWAQLGRWCADTSAPIFASTWVAVVGAARAAAHAADDIA
                     GGAPVAYAFCRPPGHHAGRSRFGGFCYLNNAAIAAHQLTTTGRRVAVLDIDLHHGNGT
                     QDIFWDRADVLYASLHIDPDVDYPYYAGFADERGGPSAEGSNLNLPLPDRCDEARYLD
                     ALDAASRAITAFGADALVVSLGTDTHQHDPIGRFDLPTTTFTSIGRRIAALGLPTAVV
                     QEGGYHLPSLGASVTGVLSAWA"
     gene            complement(7723..8712)
                     /locus_tag="PKY13_07420"
     CDS             complement(7723..8712)
                     /locus_tag="PKY13_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT79799.1"
                     /translation="MSNVRIGILGAAAIAPAALVKPARGVDRASVVAVAARDRGRADA
                     FAAKWSIPAVFDDYEALVGDPDIDAIYNPLPNSLHADWTIRALRAGKHVLCEKPLTSN
                     ADQAREVASVAASSGQVLMEAFHYRYHPMMHRAVEVVRSGELGELRHVESWMQVPLFK
                     RRDIRFDLALGGGAVMDLGCYSIHQLRSITGEEPSVVSAVAKERSGDPGVDRWTRAQL
                     RFPGGATGTFTVSLYGAMALRIGFRATGSKGELRVFNPAGPHLAHRFTVRLADGTTRR
                     ETFPKVPTYRYQLEAFCDSVLDGAPVLTGPTDSVANMEVIDAVYTAAGLPVRP"
     gene            8746..9555
                     /locus_tag="PKY13_07425"
     CDS             8746..9555
                     /locus_tag="PKY13_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT79800.1"
                     /translation="MTNLTTNALFSVAGKVVLVTGGSRGIGEMMAAGFLANGAKVYIT
                     ARKAEACDATAARLSETYDAECISIPANVAELDGIAALVAELGKRESQVDVLINNAGV
                     GWGASIDEFPEVGWDKVFDTNVKGLFFLTQQMLPLLEAGATSDDPSRVINIGSIDGIR
                     TPAFDTVSYGPSKAAVHSITRQLASKLVKRNILMNAIAPGPFPTWMLSTGVGTGGDVE
                     GTDWDAVGATMPRGRIGTPEDIAGLAIFLASRAGAFTVGEVITCDGGIVVS"
     gene            complement(9587..>10678)
                     /locus_tag="PKY13_07430"
     CDS             complement(9587..>10678)
                     /locus_tag="PKY13_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT79801.1"
                     /translation="FDDVTRILRDRTMSSDLNEAKPSAVVDLLRERSRLHEGATTIVL
                     LDDPEHARVRRLVQAPFTVRAVERLRATIVERVDAAVTEVAGRGSMELIGDLAYPLPV
                     AVFCEMLGIPVEAGPQFREWTAAVARSLDLVISEDVYDECMVLIAEMEDYLAEQVERK
                     RAEPADDVLSALIAAEIDGVGLTPDELVAQLVTLYVAGHEPTTALVGNGVAHLLDHPD
                     QLARLQADPSLVPGAVLELLRLDGPNQFVRRIALEPSTFDVPGGTVTVEPGDVVYVGV
                     GAANHDPARWGDDAAELRIDRPDAANHLQFGGGAHSCLGSHLARMQAEVTLGALFERL
                     PNLRSGGPVEWSGRMTLRSVAAVPLEWDA"
BASE COUNT         1769 a         3821 c         3451 g         1637 t
ORIGIN      
        1 ctgcagcggt tcccgaaggt ggcgaaacag ctcggtcccg ccggcagcgg gacggggcgg
       61 gacggggcga tcgtcgtgtg gtgccgaccc cggcgttgac gaactcccga ccggacgtcg
      121 gcccggacat cggcgcacac atcggcgcaa gaaccgcatt accccgccca gcagcacaac
      181 agacggcacg cacgatcggt tcacgcaccg gaaacaagcg ggcaattcga gcgacacgag
      241 ccattcctag gttgcggtca catccggtcg accctcgccg gatgaagacg ctcacgtcac
      301 agacgtgggg aggagtggta atcgatgagt cacggcagga aatacaaatg ggcgatcgcc
      361 atgatggcgg cgtccgcatt gctcttgagc gcatgcggtg acgattccgc agacgactcc
      421 gcgagcggca gctccggcgg tgacagctcc ggcacgtgcg tcgacggcga ttcggtcaag
      481 ctcggcttcc tgaactcgac ctccggcgcg atggcgatca gcgagcagac ggttcgcgac
      541 tcgctcatgc tcgccgccaa ggagatcaac gccggcggcg gcatcatgga caagaagatc
      601 gagttcgtcg aagaggacgg acagagcgag cccacggtct tcgccgagaa gatcaacaag
      661 ctcctcaccg aggatcaggt cgccgccgtc ttcggcggat ggacctctgc ttcccgcaag
      721 gcgatgctgc ccgtggtcga aggcgccaac ggcttgctct tctacccggt ccagtacgaa
      781 ggcctcgagg cctccaagaa catctactac accggcgcga cgaccaacca gcagatcatc
      841 ccggccatgg acttcctgaa gaccaaggac gtgaagacgc tgttcctcgc cggatccgac
      901 tacgtgttcc cccgcactgc caacaagatc atcaagcagt acgccaagga gctcggcatc
      961 gagatcgtcg gtgaggagta cgtgccgctc gacagcgacg actggacgac ccaggtcgcc
     1021 aagatcgccg ccgccaagcc ggacttcgtg ttcaacacca tcaacggctc gtccaacgtc
     1081 ggcttcatca aggcgtacta cgaggccgga ctcggcccgg acaactcgcc gatcatctcg
     1141 gtgtccatcg ccgaagcaga ggcacccgcc atgggtgagg acgtgaccgg ccagttcgcc
     1201 gcctggaact actgccagag cgtcgagagc cccgagaaca ccaagttcat cgaggccttc
     1261 aaggcggagt acggcgagga tcgccccacg tccgacccga tggaagcggc ctacacctcg
     1321 ctgtacctct acaagggcat ggtcgagaag gcgaagtcct tctgtgtcga cgccatcaac
     1381 gaggcctccg acggcgtgag cttcgatgct cccgaaggca aggtcaccgt gaacggtgag
     1441 aaccaccaca tcgccaagac cggtctcatc ggccagatca actcggacaa ccagttcgac
     1501 atcgtctgga gctccgacgc accgatcgag cccgatccgt tcctcgaggg ctactcgtgg
     1561 tgggacccca acgccgggtg atccccgact agtcccaacg ttccccccgg tgggcagggg
     1621 tgtcgtgcga cagcacggca tccctgccca cagcaatccg tacccccgtt cgacccgctc
     1681 aaccctcaag aagcagaggc atggaggcat tcaccacccc ctttctcacc ggcaccgcga
     1741 ccggcgccat cctcctgttg gcggcgctcg gcctgtcgct cacgttcggc cagatgggcg
     1801 tgatcaacat ggcgcacggc gagttcctca tggccggcgc ctacgccgcg tacgccaccc
     1861 agcaggtcat caccggcgcc ggcatctcca tcgtgttcgc cgtcccggtg gccttcctcc
     1921 tcgccgggct cctcggcctc atcctcgagg cctcggtcat ccagtggatg tacaagcgtc
     1981 ccctcgacac actcctcgtc accgtcggcg tgtcgctcgt cctgcagcag gcagcgaagg
     2041 acgtcttcgg cgcccagggc gacccggtga aaccgccgag ctggctgcgt ggcggactgt
     2101 ccgtcttcgg ctacagctgg ccctaccggc aggccttcac gatcgtcctg gcgttggcgg
     2161 tgctcgccgg cctcaacgcc gtcctgaagt acagctcgtt cgggcgacag atccgggcga
     2221 cggtccagaa ccgtgagctc gccgagacga tgggcgtctc gacccggaag gtcgaccgga
     2281 tcaccttctt cctcggatcc ggcatggccg gcgtcggcgg cgtcgccatc gccctcatct
     2341 ccggaaccaa cccgtccctc ggcaccacct acatcatccc ggcgttcctc gtggtggtcg
     2401 ccggcggcgt cggccagctc aagggcaccc tgatcgcagc gtgggtcgtc ggcgtcgtca
     2461 cggcgttcct gaccgactgg atcagcggaa gcatggcgca ggtcatcagc ttcgctctcg
     2521 tggtcgtctt cctccagctc cgcccacaag ggctgttcac cgtgcgcacg aggtcactcg
     2581 catgacgacc accatcgaca ccacggaggc ctccgacacc gtcgaggcca ctcccccggt
     2641 cggcacgacc gccggtcggt tctcgccggc ctacctgttc aagacctacg gcggcctcgt
     2701 cggcatcgcc ctcgtcgcga tcgtgctcct cgtgtgggcg cccaactcgc tgaccgcctt
     2761 ccgcctcggc aacctcggca agtactgcgc gtgggcgatc gccgcggtgg gcatcggact
     2821 cgcctggggc cggggcggca tgctcgtgat gggccaaggt gtgttcttcg gcctcggcgg
     2881 ctacgccatg gcgatgcaca tgaagctcga ggccgccggc cccggcaagg tgcccgactt
     2941 catggtgctc tacggcgacg cgacgatgcc gagctggtgg gagccgttcc gcagcggggc
     3001 gttcaccatc ttcgccatct tcgccatccc ggcactcatc tccttcatcc tcgggtacgc
     3061 catcctcaag cgccgggtga agggcgccta cttcgcgatc ctcacccagg cgctggcggt
     3121 cgcgttcgcg acgctgctcg tcgccaccat caagcagacc ggcgggttca acggcctcaa
     3181 caccttcacc acggtgttcg gccagaacat gtacgacccc gacgtcaaga agaacctgta
     3241 catggtggcg gcggcgatgt tgatcgtgtg cctcctcatc agctggcagc tctaccgcag
     3301 tcgctacggg gagctcctcg ttgccacgcg tgacgccgag gaacgcatcc ggttcctcgg
     3361 ctacgacccc gccaacatca agctcgtggc gttcgtcatc gccgcggtga tggccagcct
     3421 cggtggggcg ctcttcgcac cgctcgccgg catcatctcc ccgtccaacg tcgacgcaac
     3481 ggcgtcgatc atgctgctcg ccggcgtcgc gctcggtggc cgggcctcgc tgttcggtgc
     3541 ggcgctcgga gcgatcgccg tcggctatgg ccagtcgacg ctgtcggagc agttcccgac
     3601 gcagtggacc tacttccagg gagcactgtt catcgtcgtc atgctcttcc tccccggcgg
     3661 catcgcctcg ctgtggcgga aggtgtcggc cgcgttgccg aagcggtccc ccaaggaggc
     3721 caccgtggac gtcaccgtgc cgacccagac ggaggcggtc gcatgaccgc ctcattcctc
     3781 ggcgaggact acctggaggt ccgcggtctg accgtcgact tcgacgggtt caaagcgatc
     3841 agcggggtcg acgtcacctt cctgcagggg cgactccact tcctcatcgg ccccaacggc
     3901 gccggcaaga ccaccctcgt ggacgccctc accgggctgg tccacggcac gggcaccgcc
     3961 gggttccgtg gccaggacct gctgtcgatg aagtcgcaca agatcgtgcg cctcggggtc
     4021 ggccggacct tccagacggc caccgtcttc gaacagctca ccgtcctgca gaacctcgac
     4081 atcgccgccg gcctccaccg caaagcagcg ggtctcctcc tgtcacgccg gaaggtgccc
     4141 gagagcgtcg agcaggccct cgagacgatc ggcctcaccg ccctcgccaa tcgaccggcc
     4201 ggaatcctcg cccacgggca gaagcagtgg ctcgaggtcg gcatgctgct ggtccaggac
     4261 gccaaggtca tgttcctcga cgagacggtc gcgggcatga gttcagagga gcgtgaggag
     4321 accggcgaac tcctcaagcg catcgtcggc gaccgcacca tcgtggtcat cgaacacgac
     4381 atggagttca tgcgccggta cgccgacttc gtgacggtca tgcacggcgg caaggtgctc
     4441 gccgaaggaa ccgtggcgga ggtccaggcc gacaccaagg tccaagaggt ctacctgggg
     4501 accgtgacgg aggtggccgc agatgcctga gatgatgctc gagatcgacg gggtgaccgc
     4561 cggctacgga cggtcgatgg tcctgttcga cgtcagcgcc tcgatccccg tcggcggtgg
     4621 cgccgccatc atgggccaca acggcgccgg caagacgacg ttgctgcggg tcgcggtcgg
     4681 actgatcccg gtcaagtccg gcaaggtcct cctcgacggc gaggacgtca ccaacctggc
     4741 tcccaaccag cgggtcaagc gcggcctcgg ctatgtcccg cagggccaac tctccttcac
     4801 acagatgacc acgatggaga acctccagct gatcacgacc gtgaagtcgg agatcgacga
     4861 ggtgctcgac acgttccctg cgctgaccga gctcctcaag cgacctgccg gcctgctgtc
     4921 gggcggacag cgtcagcagt tggcgatcgc tcgcacgctg ctcaccaagc cacggctcct
     4981 gatcctcgac gaaccgaccg agggcatcca gcccaacgtg gtcgcggaca tcgagcaggt
     5041 catcgtcgac ctcacccagc ggggcgacct cacggtgttg ctggtcgagc agcacgtcgg
     5101 attcgcgctt cggtcgaccg acacctacta cgtgctggag tcgggtcgta tcacgggcag
     5161 cggcgccggc ggcgagggag cgctcgacga agtacgcctg gcgatggccg tctgatcgga
     5221 acgactgcgt cggccacccg gcaataggct gccgacatga catacgaagt ggacttcaag
     5281 gacgtctcga cggtcggcct cgagtcgtcc cccgtcgtcg acgcgctcgc cggcctgcgg
     5341 gccaacgaag cccgctactt caagaacaag ttcgaccaca ccttcgtcac cgagcccgcc
     5401 tcgaaggcga agacgaccat cacgtacgtc catcgcatcc tcgaggacga gcgcaacatc
     5461 gagatcgagt cacccgtgct cgaggcatcg gagttcgagg tcgacggcac ccgctgggcc
     5521 tacgtcttct acgagagcgg cctcgcgatc aacgtgatgt acgccatcgc cgacggcggg
     5581 aagcgcgccg tcgggttcaa gctctccgac ggcatggacg tccccgagga gctcgaggcc
     5641 aagttcaagt tcgcccgcca gaagtcgaag ctcgccggca ccatccgcgg ctcgtacttc
     5701 gtcatcaagg gcacgtactg accggcacga gccggtcggt acgcaacaac gtcgtaccct
     5761 gacggccatg gcgaccggtt cgatccgacg acgcgtcgcg gcgatcatcg ccggcgcctg
     5821 cctcgtcgcc accgcgtgtg ggccgccgcc gtcggcgccc gatcctgctc ctctgcgagc
     5881 cgggcaggca ccgctccgcg tcatggtggt gggtgactcg ttcggtctgt cggttggtct
     5941 cggcctcacc cactacgggg agtcgtcggg gcagatcgag gtccgcaaca gcgccttcat
     6001 cgggtgcccg ttcggccggg gcggccgcaa caacggcatc ggtctcgacc gcgactggct
     6061 gccggagtgc cgctcccagg acgagcgcct ggcgaccgtg tccgccgagt tccggcccga
     6121 cgtcgtgctg ttggccggcg gcatgtggga cgtcgccgac cggctgctcc ccggaagcgg
     6181 tcggtggacc cgcatcggtg agcccgcgta cgactggtac ctcgcgctcg agttccagca
     6241 cctctcgcgg ctgctcggca cggcgggagc aaaggtggtg tggaccaccg ctccccgctg
     6301 gaacccgcgc taccaacccg accagttcat gggacggccg ccctacgccg aggcgggcgc
     6361 cggtcgatcg gatcgctaca accaggtact gaacgctgcg gtcggaggca tgccgggggt
     6421 ccacgtgctc gatctcgccg gttggatgcg gtacttcccc ggtggcgagt tctcgcccga
     6481 cctccgcacc gacggcgtcc acctcacgcc gcagtcgacc gatgtggtgg caaacgagtt
     6541 cctcggcccg tgggtcgtcg ccatcggtcg aagctgatgg cacggcatct cccggcgcgg
     6601 gtcggggcga ccacagctca tgggggtggg tagcctccgg caatgcggac gtactggtcc
     6661 tccgatcatg tccaccacga tccgggccgg ctcccccagc ccgcgggcgg cacagcgaac
     6721 tactacagcg aggtcgcggc ccgaggcccc gccgtgctcg atgccatcga tgccgccggg
     6781 ttcggcacca tcgtcgacct ggccggcacc atcgacgccg atgtggtgcc gccggcagcg
     6841 ctggaggtcc acgacccggc gatggtggag ttcctccgca ccgccttcga ccgcatcgct
     6901 gccgaggagc agtggcccgt cgacgacacc gatggcagcg agcgcgtcgt catcgccgag
     6961 acgttcggta gaaccgacga tccatcgctg gagcggtcga cctcgccgtg ggcccagctc
     7021 ggccggtggt gcgccgacac ctccgctccg atcttcgcgt cgacgtgggt cgcagtggtc
     7081 ggcgcggcac gcgccgccgc ccacgccgcc gacgacatcg ccggcggggc accggtggcg
     7141 tacgcgttct gccgaccgcc gggccaccac gccggccgat cgcgattcgg cggcttctgc
     7201 tacctcaaca acgccgcgat cgcggcgcac cagctcacga cgacgggccg gcgcgtcgcc
     7261 gtgctcgaca tcgacctcca ccacggcaac ggcacccagg acatcttctg ggatcgggcc
     7321 gacgtgctct acgcatcgct ccacatcgat cccgacgtcg actaccccta ctacgccggc
     7381 ttcgccgacg agcggggcgg acccagcgcc gagggctcga acctgaacct gccactcccc
     7441 gaccggtgcg acgaggcccg ctacctcgac gccctcgacg ccgcctcccg cgccatcacc
     7501 gcgttcggcg ccgacgccct ggtcgtgtcg ctcggcaccg acacgcacca acacgatccc
     7561 atcgggcggt tcgacctgcc gaccaccacg ttcacgagca tcggccggcg gatcgccgcc
     7621 ctcggcctgc cgacggccgt ggtgcaagag ggcgggtacc acctgccgag cctcggcgcc
     7681 tcggtcaccg gcgtcctgtc ggcctgggcc tgaggcgccg actcagggcc gcacgggcaa
     7741 tccggcagcg gtgtacacgg cgtcgatgac ctccatgttg gccaccgaat cggtcgggcc
     7801 cgtcaggacc ggcgcaccgt cgagcaccga atcgcagaac gcttcgagct ggtagcggta
     7861 cgtcggcacc ttcggaaagg tctcgcgccg cgtggtgccg tcggccagcc gcacggtgaa
     7921 gcggtgcgcg aggtgcggac ctgccgggtt gaacacccgc aactcgccct tcgacccggt
     7981 ggcccggaac ccgatgcgga gcgccatcgc cccgtacagg gacacggtga acgttcccgt
     8041 ggccccgccg gggaaccgga gctgtgcccg cgtccagcgg tcgacgccgg ggtcgccgga
     8101 ccgttccttc gccaccgccg agacgaccga cggctcctca ccggtgatcg agcggagctg
     8161 gtggatcgag tagcagccca gatccatgac cgctccaccg ccgagcgcga gatcgaaccg
     8221 gatgtcgcgc cgcttgaaca gcgggacctg catccacgac tcgacgtggc gcaactcacc
     8281 cagctcaccg cttcggacca cctcgaccgc ccggtgcatc atcgggtggt accggtagtg
     8341 gaacgcctcc atcagcacct gccccgacga ggcggcgacc gacgcaacct cacgggcctg
     8401 gtcggcgttc gacgtgagcg gcttctcgca caacacgtgc ttgcccgccc ggagcgcccg
     8461 aatggtccag tcggcatgga gggagttcgg cagcgggttg tagatcgcgt cgatgtcggg
     8521 atcgccgacg agtgcctcgt agtcgtcgaa caccgcaggg atcgaccact tggcggcgaa
     8581 cgcgtcggcg cgcccacgat cacgagccgc caccgcaacg accgatgctc ggtcgacgcc
     8641 ccgcgccggc ttgacgaggg cagcgggagc gatggccgcc gcgccgagga tcccgatccg
     8701 aacgttcgac atcaccggag gctaactacg aatacagtcc cgcccatgac gaacctcacc
     8761 accaacgctc tgttctccgt cgccgggaag gtggtcctcg tgaccggcgg gtcgcgcggc
     8821 atcggcgaga tgatggcggc cgggttcctg gccaacggcg cgaaggtcta catcacggcc
     8881 cgcaaggccg aggcgtgcga cgccacggcg gcgcgcctgt cggagaccta cgacgccgag
     8941 tgcatctcga ttccggcgaa cgtcgccgag ctcgacggca tcgccgccct cgtcgcggaa
     9001 ctcggcaagc gcgagtcgca ggtcgacgtg ctcatcaaca acgccggcgt ggggtggggg
     9061 gcgtcgatcg acgagttccc cgaggtgggc tgggacaagg tgttcgacac caacgtgaag
     9121 ggcctgttct tcctgaccca gcagatgctt ccgctcctcg aggccggagc gacctccgac
     9181 gacccgtcgc gcgtcatcaa catcggctcc atcgacggta tccggacccc cgccttcgac
     9241 accgtgtcgt acgggccgtc caaagcagcg gtgcactcca tcacccgcca gctcgcctcg
     9301 aagctggtga agcggaacat cctcatgaac gccatcgcac ccggcccgtt cccgacgtgg
     9361 atgctgagca ccggcgtcgg taccggcggc gacgtcgagg gcaccgattg ggacgcggtc
     9421 ggcgccacca tgccacgcgg tcgcatcggc acccccgagg acatcgccgg cctcgcgatc
     9481 ttcctcgcgt cccgcgccgg cgcgttcacc gtcggcgagg tcattacctg cgacggcggc
     9541 atcgtcgtct cctgacagcg gggcgcagcc cgaggcgccc gaccggtcag gcgtcccact
     9601 ccagcgggac cgcggcgacg ctccggagcg tcatgcgtcc ggaccactcg acgggtccgc
     9661 cggaccggag gttcgggagg cgctcgaaca acgctcccag tgtgacctcg gcctgcatgc
     9721 gggcaaggtg cgagccgagg cagctgtggg cgcctcctcc gaactgcaga tggttcgcag
     9781 cgtcggggcg atcgatgcgc agttcggcgg cgtcgtcgcc ccagcgcgcc ggatcgtggt
     9841 tggcggcgcc gaccccgacg tagacgacat cgccgggctc gacggtgacg gtgccaccgg
     9901 gtacgtcgaa ggtcgacggt tcgagcgcga tcctgcggac gaactggttc ggaccgtcga
     9961 gccggagcag ctccagcacc gcgcccggca cgagtgacgg gtcggcctgc aagcgggcga
    10021 gctggtccgg gtggtcgagc aggtgcgcga cgccgttgcc aacgagcgcg gtggtcggtt
    10081 cgtggccggc gacgtacagc gtcacgagct gggcgacgag ctcgtcgggg gtcagcccga
    10141 cgccgtcgat ctcggccgca atcaacgccg acagcacgtc gtcggcgggc tcggcccgct
    10201 tccgctccac ctgctcggcc aggtagtcct ccatctcggc gatgagaacc atgcactcgt
    10261 cgtacacgtc ctcggagatg accaggtcga gactgcgtgc caccgcagcc gtccactccc
    10321 ggaactgcgg cccggcctcg acgggaatgc ccaacatctc gcagaacacc gcgacgggga
    10381 gcgggtaggc gaggtcgccg atcaactcca tcgatccgcg gccggccacc tcggtcaccg
    10441 cggcgtcgac gcgctcgacg atcgtcgctc gcaggcgctc gacagcccgc accgtgaacg
    10501 gagcctgcac caggcggcgc acgcgggcgt gctcggggtc gtcgagcaac acgatcgtcg
    10561 tggcgccctc gtggagacgg gaacgctcac ggaggaggtc gaccaccgcc gacggcttcg
    10621 cctcgttgag gtcactcgac atcgtccggt cccggaggat gcgggtcacg tcgtcgaa
//
LOCUS       DAOSOA010000087        10668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6197_length_10668_cov_6.673137, whole genome shotgun sequence.
ACCESSION   DAOSOA010000087 DAOSOA010000000
VERSION     DAOSOA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6197_length_10668_cov_6.673137"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKY13_07435"
     CDS             <1..904
                     /locus_tag="PKY13_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT79802.1"
                     /translation="AHHHGAAAMRAGARHQRPLAIAFALTLGFLVVQVVTGFATNSLA
                     LLSDAGHMATDVLGLGMALAAIRMASRGSQTQHNTFGVYRLEILAALTNAVLLVGVAA
                     YVMFEAISRLGEPPHIASAPVLIVGVLGLVVNVVAFLLLRSGSKESLNVEGAYVEVMS
                     DALGSLGVILGAGVMWITGWAWVDPVIAAGIGLFILPRALRLGREALRVLLQAAPRNL
                     DLAAVTADLAGIDGVVDVHDLHVWTLTSDMDVLTAHLMTSPSTDSHAVLDRAREMLGE
                     RHGLHHATLQVEPDDHRGCDELEW"
     gene            943..1305
                     /locus_tag="PKY13_07440"
     CDS             943..1305
                     /locus_tag="PKY13_07440"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT79803.1"
                     /translation="MASRFYHLCIDAHDPSALARFWAAVLDQPILYEDTDEVLVGADL
                     QAYPGLCFLPVSDEKVVKNRLHIDLAPDDADAELDRLIALGATLVDIGQGDVTWTVLA
                     DPEGNEFCLLQPKSSLTG"
     gene            complement(1357..1431)
                     /locus_tag="PKY13_07445"
     tRNA            complement(1357..1431)
                     /locus_tag="PKY13_07445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1396..1398),aa:Gly,seq:tcc)
     gene            1660..2556
                     /locus_tag="PKY13_07450"
     CDS             1660..2556
                     /locus_tag="PKY13_07450"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOT79804.1"
                     /translation="MTLVSAVFVASGCSGGDDAATDAAEAAPPAVAGDRDTSAGGAAD
                     AGELVGVARELVSTADLTVVAKDVEEASAAAEAAVTERGGFVFGQEDAFEGEDQAVLT
                     LKVPPKDFRGLLVALADLGKVEQRSVTTDDVTAAGADLDARILSAQKSVDRVRGFLDQ
                     TKNVTELAGLEAELTRRESDLEQLVGQRRVLDDQVSLATVTLRLSSTPAPPPPVGDSL
                     RSVPGFGGALASGVGALVKIGGVVGAGVGYALPFAVVLGVPMLAVRALRRRRRDGASP
                     GPTGPTPSLTDPPAPPPMAPIA"
     gene            complement(2577..3239)
                     /locus_tag="PKY13_07455"
     CDS             complement(2577..3239)
                     /locus_tag="PKY13_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOT79805.1"
                     /translation="MSCDTWIEALSAAADGEDPGVDPRLVAAHLTRCEPCRAFATELE
                     GVDVASRVAVVPAAPDLARQVARSAAVADRARVSGVLRALLGGIAIAVGVSSIPPLIF
                     GDDGMGTGMSHAARHFGAFSMAYAVGLLVVTVRPARARTMLPVAFVVASALAVTAVFD
                     AAEGRVTFIAEGRHIPELVSLALVWLLTRANVAAGAVAPHTTVPLRLVDHDISPDRRE
                     VG"
     gene            complement(3306..3794)
                     /locus_tag="PKY13_07460"
     CDS             complement(3306..3794)
                     /locus_tag="PKY13_07460"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOT79806.1"
                     /translation="MNPTIVERHRPPQRHRPRLALATLLVGLLGLAACGGDGSVIEIT
                     DAVMPVPGSADETSVYLTITNSGDQDDQLLNADTEIAPMTHLHQTEIETSGRTSMTTS
                     NAVDVAAGSSVRFAAGGRHLMLMGPKELQPGDTFILWLNFRHAGTVKTTVKVIDIGDL
                     DL"
     gene            3902..4702
                     /locus_tag="PKY13_07465"
     CDS             3902..4702
                     /locus_tag="PKY13_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79807.1"
                     /translation="MSIRRTLVRLSLIVSLPLAVVGLITSAAAAHGGDITIEASGSVS
                     GTTVTYSVHMIWEDGHDVIGQGVVINASGPGGSRSATLSPTNSSGRTSGSIDLGEGTW
                     SVTFNTDGGSRTISQRIGAEPVATAAPAPAPAPTAAPSPAPTAAPAPPTTRRAASGST
                     TTTRPGATTTSVAVADTTPTTVAVAAETTAVPDTSTTTASTSTSTTTSSIVVTEIASS
                     STKDDDGGSAGLIVALVVVALIAVGGGAAFWRRRVSAPAGASVDGPGE"
     gene            complement(4659..6125)
                     /locus_tag="PKY13_07470"
     CDS             complement(4659..6125)
                     /locus_tag="PKY13_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOT79808.1"
                     /translation="MCSAVPSSPPRRSDPPGFDREPDSERPAISGTPHSARNELRRAL
                     WRVHFYAGMLTMPVLVLLANTGIVMLYGDSITRWLDPDVKSVEVGSTETSLDAQLAAV
                     QARFPTGAVREIKTATTPTSPTVFLISGTEQDELVFVDPYDARVLGSRTTGTGAVQLA
                     TRLHAFLDVDAPIVSIPTVAALFDDGPGTIPISLSDAALEITAGWAIVLVASGVILWR
                     RPRDRRARGVSSRRSFWRRAHATPGIVLAAGLMVSLLTGMLWSTYWGATWRRIADAAT
                     PNQPFRAPDSTMSMMGMDGDHGSMAMAPITLNTVAATAAELGLDAGYSIVLPRDGAAP
                     DGSPTFGTFELRVDWPTRVSQNRSYYIDQFDGALLSVRTTDQLGALATVSEIGKQTHV
                     GVQFGVVSRVLLTFVCLAVDWSAMSALAMWLRRRSRSGGLALPRRPVTTSQVRQLRSA
                     AVVMGVVFPLWGASALALWALDVAVIRRVRRPRHRPER"
     gene            complement(6085..7002)
                     /locus_tag="PKY13_07475"
     CDS             complement(6085..7002)
                     /locus_tag="PKY13_07475"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HOT79809.1"
                     /translation="MIRKRSLLIGASAVAALVVSAVPASAHVSPTTYGTTYTAGVTNV
                     FALRVPHSCADADTVNSPGGTSLTSKLVVTVPASVTSVKPQFIPGWTLSTVKSPTGAV
                     TEITWQASSAAFAIPDTSYMDFGIRATLNGAAGDKISLPTYQYCDFHENGTAATGLVD
                     PWIGADAPTITLVGADKAVNNPAQIAEVKSKAFSVDAVAADVATIKTSLESTGRQITF
                     LIAVVNQLALNRGEVAASRSAGKVNVNIDLSAVHKGRSVDVLLNGVRLGSKALNAAGD
                     LSTSFKGSAGPNSKIDVVLGGTVVATTTF"
     gene            complement(7157..7522)
                     /locus_tag="PKY13_07480"
     CDS             complement(7157..7522)
                     /locus_tag="PKY13_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT79810.1"
                     /translation="MPTPATANDTADRDATSTTPAMCCGRLTDEPLTPDEATDLAHAF
                     AALGDPIRLRLYSLVASADEICSCDLMPLLDRSQPTVSHHTKALADAGLIVGEKRGRW
                     VYWRAVPEARESLRRTLGD"
     gene            7625..8104
                     /locus_tag="PKY13_07485"
     CDS             7625..8104
                     /locus_tag="PKY13_07485"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HOT79811.1"
                     /translation="MSRVQLALNVSDLDAAVEFYSKLFNAPVNKRKPGYANFAIAEPP
                     LKLVLIEGEGGGTLNHLGVEVETADEVEAAERRLSNDGLATTGIDDTMCCYALKTETW
                     VSDPDGAPWEFYVKTGDAEQMTNTILADGENAQCCAPAASAPVSIGAVSDAGSGGCC"
     gene            complement(8109..8633)
                     /locus_tag="PKY13_07490"
     CDS             complement(8109..8633)
                     /locus_tag="PKY13_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT79812.1"
                     /translation="MTSPGSLRLLLAASAEGDDVAMAEFVRRTQPDVWRLCEALGSTG
                     EVEDLVQETYLQAVRSAAGFRGDGSPLAWLLSIARNVCADHVRRRQRSRRLVERLIPE
                     VVDHVPAVDRSTPQLLDGLAADRREAFALTQIVGLSYEEAAEVIGCPIGTVRSRVARA
                     RSDLLAAVRRSEAM"
     assembly_gap    8866..8965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8966..9641
                     /locus_tag="PKY13_07495"
     CDS             <8966..9641
                     /locus_tag="PKY13_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79813.1"
                     /translation="DAGSDHKVMSCEDCREAISDMVDDRLPSVDPVLEEHLEGCAECR
                     EFAEMSYRLRRRAAIADAPNVPDLSKAVVRNVGIADRERHSPIVRWLLVLVAIQIVVF
                     AIPDFLATAAEAHALSHLGAFSLAYAFGLVVVAIRPARARTMLGVSLVLIAALIATAV
                     EDIITGRTLLVSESVHLLEVCSAFLVWLLTRPGRGLRQTTAEPAAKGDGQHAEPSLRL
                     VTHPEP"
     gene            9645..>10668
                     /locus_tag="PKY13_07500"
     CDS             9645..>10668
                     /locus_tag="PKY13_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79814.1"
                     /translation="MGATPLGVFARAVSVLVAAIGCVVVLAPPASAHGPTGSGAPSSN
                     YRVTVTAVPELAGLSVELSDGGTEFVVTNRSAADLVILDYSGSPYLRIDDTGVWENRN
                     SQAVVLNSQMLPPEVEPLADPTISPDWHRLSTGHTARWHDHRAHWMDDDPAAVRAAPD
                     RTHVVQSWSIPVEVDGRVDLISGTIEWVPPPLLGGWLVVVAAVALVVAVALVWRTRPA
                     LVASTLVLGAVLVTTMVGIWADSSEPTTSKLALATLPILVPALLGGAAFTWGERGTEP
                     LWLAMLAGLLVVGSAAIGSGAWLAESQLPTSLDADLARWLVAGSVGVGLGVGGFSAWR
                     LIATRAQ"
BASE COUNT         1575 a         3497 c         3791 g         1705 t
ORIGIN      
        1 cgcccaccac cacggcgcgg cagcgatgcg cgccggggcc cggcaccagc gtccgttggc
       61 catcgccttc gcactgaccc tcggcttctt ggtcgtgcag gtcgtcacgg ggttcgctac
      121 caactccctc gccctgctct ccgacgccgg acacatggcc accgatgtcc tcggcctcgg
      181 catggcgctc gccgcgatcc gaatggcgtc tcggggaagc cagacccagc acaacacgtt
      241 cggtgtttac cgtctcgaga tcctcgcggc gctcaccaac gcggtcctgc tcgtcggcgt
      301 cgccgcgtac gtgatgttcg aggccatctc tcggctcggc gaaccgcccc acatcgcgtc
      361 ggcacccgtg ctcatcgtcg gagtgctcgg gctcgtggtg aacgtcgtgg ccttcctgct
      421 gctccgctcg gggtcgaagg agtcgctcaa cgtcgagggc gcgtacgtcg aggtgatgtc
      481 cgatgcgctc ggctcgctcg gcgtcatcct cggcgccggc gtgatgtgga tcaccgggtg
      541 ggcgtgggtc gatccggtca tcgctgccgg gatcgggctg ttcatccttc cccgggcctt
      601 gcgtctcggt cgtgaggcgc tgcgcgtcct gttgcaggcg gcgccgcgga acctcgatct
      661 cgccgccgtg accgccgacc ttgccggcat cgacggggtg gtggacgtgc acgacctcca
      721 cgtgtggacc ctcacgtccg acatggacgt gctcactgcg cacctcatga cctcgccctc
      781 gaccgacagc cacgccgtgc tcgaccgtgc tcgcgagatg ttgggggagc ggcacggctt
      841 gcaccacgcc acactccagg tcgaacccga cgaccaccgc gggtgcgacg aactcgagtg
      901 gtagtgcgtt cggagttcgc tgtcatcggg taacccggtc gtatggcttc gcgcttctac
      961 cacctgtgca tcgacgccca cgatccgtcg gcgctcgccc ggttctgggc ggcggtcctc
     1021 gaccagccga tcctgtacga ggacaccgac gaggtcctcg tcggggcaga tctgcaggcc
     1081 taccccggct tgtgcttctt gccggtgtcg gacgagaagg tggtcaagaa tcggctgcac
     1141 atcgacctcg cgcccgatga cgccgacgcc gaactcgacc gcctcattgc gctcggtgcg
     1201 acgctggtcg acatcgggca gggcgacgtg acctggacgg tgctcgccga tccggaaggc
     1261 aacgagttct gcctgttgca gccgaagtcg tcgctcacgg gctgacgcga acggcgggcg
     1321 accgccgagt tcggcgacac gcccgccggc gtccggtgga gcgggtgacg gtaatcgaaa
     1381 ccgcatagcc tgcttggaag gcaggggctc tagccattga gctacacccg caaggcggcg
     1441 ctatgttagc gcccggccgc ctgagaatgg ttcgcatctg ctgagggcct tcgaagtgac
     1501 cgccgtcaca ccggctgccg ccggttctac ggtcgtcggc gaggtgtcgg ggcggggaga
     1561 tgaggtgggt cggggaaccg atcggcttcg gtcgtcgtcc tatccgcacc ttcgggcagg
     1621 cgacgaaacg gggaactcat gcggggacga tggttgacgg tgacgttggt gagcgcggtg
     1681 ttcgtggcct cgggctgcag cggcggtgac gatgccgcga cggatgctgc cgaggcggca
     1741 ccacctgcgg tcgctggcga tcgggacacg tcggcgggcg gcgcggccga cgccggagaa
     1801 ttggtcggcg tggcgcgtga actcgtctcg acggccgacc tgacggtcgt tgcgaaggac
     1861 gtcgaggagg cctcggctgc ggcggaggcg gcggtcaccg agcggggcgg cttcgtgttc
     1921 ggccaggagg atgcgttcga gggtgaggac caggccgtcc tcacgctcaa ggttccgccc
     1981 aaggacttcc gtggcctgtt ggtagcgctc gccgacctcg gcaaggtgga gcaacggagc
     2041 gtgaccaccg acgacgtcac cgccgccgga gcggatctcg acgctcgcat cctgtcggcg
     2101 cagaagagcg tcgaccgagt ccgcgggttc ctcgaccaga cgaagaacgt gacggagctg
     2161 gcgggtctcg aggctgagct cactcgccgc gagtccgatc tcgaacagct ggtcggccag
     2221 cgacgggtgc tcgacgatca ggtgtcgttg gcgactgtca cgctgcgcct cagttcaacc
     2281 ccggcgccac cgcccccggt cggcgactcg ctgcgcagcg tgccgggctt cgggggtgcc
     2341 ctcgcaagtg gggtcggcgc gttggtgaag atcggtggtg tggtcggcgc cggagtcggc
     2401 tacgccttgc ccttcgccgt ggtcctcgga gtgccgatgt tggcggtgcg tgctctgcgc
     2461 cgtcgccgcc gggatggtgc gtcgcccggc ccgacgggtc cgacgccctc gctgacggac
     2521 ccgccggctc cgccccccat ggcacccatc gcctgacagc tgcgcggatc gtcgggttac
     2581 ccgacctcgc gccggtcggg gctgatgtcg tggtccacga ggcggagcgg cacggtcgtg
     2641 tggggtgcga cggcgccggc ggccacgttg gcccgtgtga gcaaccagac cagggcgagg
     2701 ctcaccaact cgggaatgtg tcggccctcg gcgatgaagg tcacgcggcc ctcggcggcg
     2761 tcgaagaccg cggtgacggc gagcgccgag gcgacgacga aggcgaccgg caacatcgtt
     2821 cgcgcccggg ccgggcggac ggtcaccacg agcaggccga cggcgtacgc catgctgaac
     2881 gctccgaagt gtcgggccgc gtgggacatg cccgtgccca tgccgtcgtc cccgaagatg
     2941 agcgggggaa tcgacgacac gccgactgcg atggcgatgc cgccgagtag tgctcggagc
     3001 acaccactca cgcgggcgcg gtcggcgacg gcggcgcttc gagcgacctg ccgagccagg
     3061 tcgggggccg ccgggaccac ggcaacccgt gaggcgacgt cgaccccctc gagctcagtg
     3121 gcgaatgccc ggcacggctc gcacctcgtg agatgtgcag cgacgagtcg agggtcgacg
     3181 ccggggtcct ctccgtcggc cgccgccgac agcgcctcga tccaggtgtc gcagctcatg
     3241 gaaccaaggt cgtcgcggac gccgggtgag ttccacgacg caccgagaac accaccgtcg
     3301 tcgtctcaga ggtcgaggtc gccgatatcg atcaccttga cggtggtctt cacggtcccg
     3361 gcgtgtcgga agttgagcca caggatgaac gtgtcacccg gctggagttc cttggggccc
     3421 atcaacatca ggtgacgtcc gccggcggcg aagcgaacgc tcgatccggc cgccacgtcg
     3481 acggcgttgc tcgtggtcat cgacgtccgg ccactggtct cgatctcggt ctggtgcaga
     3541 tgggtcatcg gggcgatctc ggtgtcggcg ttgagcagct ggtcgtcctg gtcgcccgag
     3601 ttcgtgatcg tcaggtacac cgacgtctcg tcggccgatc ctggcaccgg catcaccgca
     3661 tcggtgatct cgatgaccga tccgtcaccg ccgcacgcgg cgagtccgag gagcccgacc
     3721 agcagtgtcg cgagcgcgag ccgaggtcga tgtcgctggg gtggtcggtg gcgttcgacg
     3781 atcgttggat tcacagacag ggagtgcaca ggaagtggtc ggcgcgaacg tctctggggt
     3841 tcccgacggt gtcgctcgtc acgtcgggaa ctttcggggc ccggatcccg acctgagggg
     3901 catgagtatt cgtaggaccc tggttcgtct gtcgctgatc gtctcgctcc cgctcgcagt
     3961 ggtcggcctg atcacgtccg ctgcggcggc gcacggtggc gacatcacca tcgaggcatc
     4021 ggggtcggtg agcggcacga ccgtgaccta cagcgtgcac atgatctggg aggacggcca
     4081 tgacgtgatc ggccaggggg tggtcatcaa cgcgtcgggc cccggcggga gtcgttcggc
     4141 aacgctgtct ccgacgaact cgtcgggtcg gacgtcgggc tcgatcgacc tcggggaggg
     4201 cacgtggtcg gtcacgttca acaccgacgg aggatcccgg acgatcagcc agcggatcgg
     4261 tgcggagccg gtcgcgaccg cagctccggc tccggctccg gccccgacgg cggcgccctc
     4321 acccgctcca accgcggcgc cggccccgcc gacgacccgc cgggcggcat cggggtcgac
     4381 gacgacgacc cgtcccggtg cgacgacgac ctcggtcgcc gttgccgaca ccaccccgac
     4441 gacggtggcg gtcgctgccg agacgaccgc ggtgcccgac acctcgacca ccaccgcctc
     4501 gacatcgacc tcgacgacga catcgtcgat cgtggtgacc gagattgcgt cgtcctcgac
     4561 gaaggacgac gacggcggct cggccgggtt gatcgtcgca ctcgtcgtcg tggcgctgat
     4621 cgctgtcggt gggggagctg cgttctggcg gcgtcgggtc agcgctccgg ccggtgcctc
     4681 ggtcgacgga cccggcgaat gaccgcaacg tcgagcgccc acagcgccag cgcggaggcg
     4741 ccccagagcg ggaagacgac ccccatgacg acggctgcgg agcggagctg acgcacctgg
     4801 gacgtggtga ccgggcggcg cgggagcgcc agtcctcccg atcgtgaacg gcgtcgcagc
     4861 cacatggcga gcgccgacat cgcgctccag tcgacggcga ggcagacgaa cgtgaggagc
     4921 accctcgaca cgaccccgaa ctgcacaccg acgtgggtct gcttgccgat ctcgctgacc
     4981 gtcgcgagtg ctccgagctg gtcggtggtg cgcacggaga gcagcgcgcc gtcgaactgg
     5041 tcgatgtagt agctgcggtt ctgtgacacg cgtgtcggcc agtcgacgcg gagttcgaac
     5101 gtgccgaagg tcggcgaacc gtccggggct gcgccgtcgc ggggcaggac gatgctgtag
     5161 ccggcgtcga ggcccagctc cgcggcggtg gcggcgacgg tgttcagcgt gatcggcgcc
     5221 atcgccatcg acccgtggtc gccgtccatc cccatcatcg acattgtcga gtcgggtgct
     5281 cggaacggct ggttcggggt cgccgcgtcg gcgattcgtc gccaggtcgc gccccagtag
     5341 gtcgaccaca acatgccggt caggagcgac accatcaggc cggcggcaag gacgatgccg
     5401 ggggtcgcgt gggcgcggcg ccagaacgat cggcggctgc tcacgccgcg ggcgcgccgg
     5461 tcccgcgggc gtcgccagag gatgaccccg ctggcgacca gcacgattgc ccatccggcg
     5521 gtgatctcga gcgcggcgtc actgagcgag atcgggatcg tgccgggccc gtcgtcgaac
     5581 agggcggcga cggtcgggat gctcacgatg ggcgcgtcga cgtcgaggaa cgcatggagc
     5641 cgggtcgcca gctgcacggc gcccgtgccg gtggtgcggg acccgagcac acgtgcgtcg
     5701 tagggatcga cgaagaccag ctcgtcctgt tcggtgcccg agatcaggaa gaccgtcgga
     5761 gacgtcggcg tcgtggcggt cttgatctcc cggactgcgc cggtggggaa ccgtgcctgg
     5821 accgcggcca gttgggcgtc gagcgacgtc tcggtcgatc cgacctccac cgatttcacg
     5881 tcggggtcga gccagcgggt gatcgagtcg ccgtacaaca tgacgatgcc cgtgttggcc
     5941 aacaggacca acacgggcat cgtcaacatg cctgcgtaga aatggaccct ccacagcgcc
     6001 cgccggagtt cgttgcgggc gctgtgggga gtaccggaga tggcaggcct ttcggagtcg
     6061 ggttcgcggt cgaaccccgg tggatcagaa cgtcgtggtg gcgacgacgg taccgccgag
     6121 cacaacgtcg atcttcgagt tcggaccggc cgagcccttg aacgacgtcg acaggtcgcc
     6181 ggcggcgttc agcgccttgc tgccgagccg caccccgttg aggagcacgt cgaccgagcg
     6241 gcccttgtgc acggcggaca ggtcgatgtt cacgttcacc ttgccggcgg agcgggaggc
     6301 ggcgacctca ccccggttca gggcgagctg gttgacgacg gcgatcaaga acgtgatctg
     6361 ccggccggtg gactcgagcg acgtcttgat cgtggcaacg tcagcggcaa cggcgtcgac
     6421 gctgaatgcc ttcgacttca cctcggcgat ctgcgccggg ttgttcacgg ccttgtcggc
     6481 accgacgagg gtgatggtcg gggcatccgc gccgatccac gggtcgacga ggccggtggc
     6541 ggccgtgccg ttctcgtgga agtcgcagta ctggtaggtc ggcaggctga tcttgtcgcc
     6601 ggcggcgccg ttgagcgttg cccggatgcc gaagtccatg tacgacgtgt cggggatcgc
     6661 gaacgccgcg gagctggcct gccaggtgat ctcggtcacg gcgccggtcg gcgacttcac
     6721 ggtcgagagc gtccagccgg ggatgaactg cggtttcacc gaggtcaccg acgccggaac
     6781 cgtcacgacg agcttggagg tgagcgacgt gccgccgggg gagttcacgg tgtcggcatc
     6841 ggcgcacgag tggggcacac gcagcgcgaa gacgttggtg acgcccgcgg tgtaggtcgt
     6901 gccgtaggtc gtgggggaga cgtgagccga tgccggaact gccgagacga cgagcgccgc
     6961 cactgcggac gcgccgatga gaagagatcg ctttcggatc atgaggagcc tttcgagctg
     7021 tcagatgggg gtgacgtccg gatggtgcga ccgatcgcgg ttcgtcgtgt aactgagtcg
     7081 ctcgggatgg cctgacagtt cccggagatc tcagaaacga cgtcggctgc gcacccgggg
     7141 tccgcgagcg gtgcggtcag tcgcccagcg tgcggcgcaa cgactcgcgg gcctcgggga
     7201 cggcgcgcca gtacacccac cgtccccgct tctctcccac gatgagtccg gcgtcagcga
     7261 gcgccttcgt gtggtggctc accgtcggtt gtgagcggtc gagcagcggc atgagatcgc
     7321 acgagcagat ctcgtccgcg gacgccacca ggctgtacag gcggaggcgg atcggatcgc
     7381 cgagcgcggc gaacgcgtgg gcgaggtcgg tggcctcgtc gggggtgagg ggctcgtcgg
     7441 tgaggcgacc gcaacacatc gccggcgtgg tgctggtggc gtctcggtcg gccgtgtcgt
     7501 tggcggtcgc tggggttggc acgagaagtt cctcccaata cattgacatc catcgatgca
     7561 ttcgcgatga tcaatacatc gacatccgcc aatgtaacgg attccgacca cgaggagcat
     7621 cccgatgtca cgagttcaac ttgccctgaa cgtctccgat ctcgacgcag ccgtcgagtt
     7681 ctactccaag ctgttcaacg ccccggtcaa caagcgcaag cccggctacg ccaacttcgc
     7741 catcgccgag cctccgctca agctggtcct catcgagggc gaggggggcg gcacgctcaa
     7801 ccatctgggg gtcgaggtcg agaccgccga tgaggtcgag gcggccgagc gccgactgtc
     7861 gaacgacggt ctcgcgacga ccggcatcga cgacacgatg tgttgctacg ccctcaagac
     7921 cgagacgtgg gtgagtgatc ccgacggggc accgtgggag ttctacgtca agaccggcga
     7981 cgccgagcag atgaccaaca cgatcctcgc ggacggcgag aacgcccagt gctgcgctcc
     8041 ggctgccagc gcacccgtct cgatcggggc ggtgtcggac gccggcagcg gcgggtgctg
     8101 ctgatacgtc acatcgcctc gctgcggcgg acggcggcga gaaggtcgga gcgggcccgg
     8161 gcgacccggg acctgacggt gccgatcggg cagccgatga cttctgcggc ctcctcgtag
     8221 gacaacccca cgatctgggt gagggcaaac gcctccctcc ggtccgcagc gaggccgtcg
     8281 agcagttgcg gagtgctccg atcgaccgcg gggacgtggt cgaccacctc cgggatgagt
     8341 cgctcgacga gtcggcgcga gcgttgacgg cgccgcacgt gatcggcgca cacgttccga
     8401 gcgatcgaca acagccaggc aagcggtgat ccatcacccc gaaaaccggc ggccgaacgg
     8461 accgcctgga ggtaggtctc ctggacgagg tcctcgacct cgccggtgga cccgagggcc
     8521 tcgcacagtc gccagacgtc cggctgcgtc cgccgcacga actccgccat cgcaacgtcg
     8581 tcaccctcgg ccgaggcggc caggaggagg cgaagcgagc cgggcgatgt cacgactgca
     8641 tcgttgcacc gacgtcgccc ggttcggcaa atcgtgcgaa ccgccgccga acgggcgggc
     8701 gatcgtcgcc gctcgatgaa ctcgggaact tttccgacgt tcggggcgac tactgggtag
     8761 ccgctctgat gggggtggca ccgtcaccgg ttcggacaac ggggacgagg aacttgcacc
     8821 cgtgctcggg gccgtcgggg gagtagtgac ccccggtggc cccgannnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnncgacg ccgggagcga ccacaaggtg atgagttgcg
     9001 aggactgccg agaggccatc tcggacatgg tcgatgaccg gctgccgtcg gtcgaccccg
     9061 tcctcgagga gcacttggaa ggctgcgccg agtgccgcga gtttgccgag atgtcctacc
     9121 gcctgcgccg acgcgccgcg atcgccgacg ctccgaacgt gcccgacctg tcgaaggcgg
     9181 tcgtccgcaa cgtcggaatc gccgaccgtg agcgccacag cccgattgtg cggtggctcc
     9241 tcgtgctcgt cgcgatccag atcgtcgtct tcgcaattcc cgactttctc gccaccgccg
     9301 ccgaggccca tgcgttgtcc cacctcggtg cgttcagcct cgcctatgcg ttcggcctcg
     9361 tcgttgtggc gatccgcccg gcgcgggccc gcaccatgct cggagtgtca ttggtgttga
     9421 tcgcggcact catcgcgacc gcggtcgagg acatcatcac ggggcgaacc cttctcgtca
     9481 gcgagtcagt gcacctcctc gaggtgtgca gcgccttcct cgtgtggctg ctcacccggc
     9541 ccggtcgtgg acttcgccag acgacggccg agccggcggc caagggcgac ggtcaacacg
     9601 ccgaaccgtc gctgcgtctc gtgacccacc cggagccctg atcggtgggt gcgactccgc
     9661 tgggagtgtt cgcccgagcc gtctccgtcc tggtggccgc catcggttgc gttgttgtac
     9721 tcgcacctcc ggcgtcggcc catgggccga ccggttccgg tgccccgtcc tccaactacc
     9781 gcgtcacggt caccgcggtg ccggagctcg ctggcctcag cgtcgagctg agcgacggcg
     9841 ggacggagtt cgtcgtgacc aaccggtcgg ctgcggatct cgtgatcctc gactactcgg
     9901 gctccccgta cctgcgaatc gacgacaccg gagtgtggga gaaccggaac tcccaggcgg
     9961 tcgtgctgaa cagccagatg ctcccgcccg aggtcgagcc actcgcggat ccgacgatct
    10021 ctcccgattg gcaccggctg tcgacaggac acacagcgcg gtggcacgac caccgtgctc
    10081 actggatgga tgacgacccc gctgcggtgc gcgcagcgcc cgaccggacc cacgtcgtgc
    10141 agtcgtggtc gataccggtc gaggtcgacg ggcgggtgga tctgatctcc ggaacgatcg
    10201 aatgggtgcc gcctccgttg ctcggggggt ggctggtggt ggtggctgcg gtggcgctcg
    10261 tcgtcgccgt tgcactggtg tggcgaacgc ggccggcgtt ggtcgcctcg accctggtgt
    10321 tgggcgcggt gctcgtgacc accatggtcg ggatctgggc ggacagctcg gagcccacga
    10381 cgtccaagct cgcgctcgcc acacttccga tcctcgtgcc cgccctgctg ggcggggcgg
    10441 cgttcacctg gggcgagcgg ggaaccgaac cgttgtggct tgccatgttg gcggggctgc
    10501 tcgtcgtggg gagtgccgcg atcggcagcg gcgcgtggct cgccgagtcc cagttgccga
    10561 cctcgctcga cgccgatctc gcgcgctggc tggtcgccgg ctccgtcggt gtcggcctgg
    10621 gcgtcggcgg attctcggcg tggcgactga tcgcgacccg agcgcagg
//
LOCUS       DAOSOA010000088        10653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6217_length_10653_cov_4.928100, whole genome shotgun sequence.
ACCESSION   DAOSOA010000088 DAOSOA010000000
VERSION     DAOSOA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6217_length_10653_cov_4.928100"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="PKY13_07505"
     CDS             <1..154
                     /locus_tag="PKY13_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOT79815.1"
                     /translation="LNTSSITLDFPPPRGALSHQALGPWLEFYNHRRPHSALGHTTPA
                     SRLTAD"
     gene            187..1062
                     /locus_tag="PKY13_07510"
     CDS             187..1062
                     /locus_tag="PKY13_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79816.1"
                     /translation="MGVVTSEELRQRQNPDGSFGQQATPTGRVVTTIFAARSLQEAGL
                     VDHPSLGPALDFLARTATCGGCTITGQRDGVLSCYTGMLARLMVRSRRLDDAVPLLEW
                     IVTYQPVVYGDTTYHQPTGPIWGEYLRHRYGGCMAATTCLLGLVPACGALLDARDAGI
                     DVPVDAHLDAMRRLLIDRRLMHRTDGTIIALSGHTKADPHGTRWMLPAFPRDYLIDLI
                     ELVDLAQRLHVPDSAMTEAVDLIASWQLPDGSWPTLASRRVADAYRPDPVNRHRASAI
                     TTARVHSLGLDLNDR"
     gene            1120..1674
                     /locus_tag="PKY13_07515"
     CDS             1120..1674
                     /locus_tag="PKY13_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT79817.1"
                     /translation="MTVLETGRFRLRPPTSHDVPALVEHWRDGAVREYLWDDKLVDVR
                     VVEAVVAASESDHATSGWGGWVIRTRDEEAPLVGWCGLRVGPPDGSAASELLVELTYS
                     LVPTWWRTGATVEASTAVLDHAFANSTLDEVFGGVDAPNLRSVATLRRLGFVPDREVQ
                     LDVGPTPYWRLGRADWLDRRHRPA"
     gene            complement(1716..2414)
                     /locus_tag="PKY13_07520"
     CDS             complement(1716..2414)
                     /locus_tag="PKY13_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79818.1"
                     /translation="MNLYRTLDTEYRHLRRQGVPEHWRLDVPSIDALVETIRTDSPCS
                     DQLLGRLIELAGRDQRAVVVAAYALVPLLRARLGRTVTDEYRSDALTELTIVLLDAPT
                     VGTHLARRLVNRAHTRVHKAARRVTHRGVVNLVEITPTDPDLLARRRDLAHDDLADDV
                     AARVDLARFGNAVADAVDAGALSPVLWDAFRDHRIRRALDTTTPACSAVQRKLASRAS
                     ARLRPLVDEYLHAA"
     gene            2540..4777
                     /locus_tag="PKY13_07525"
     CDS             2540..4777
                     /locus_tag="PKY13_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HOT79819.1"
                     /translation="MNRTPERAAHSPGADIALVAIGGVLIAIGAITWGGAGLAATVTG
                     GQLHGGLADAFGALGRLGSDPSQPAAAWGKNATGLPGPVAYWACTALVAVAVAVLAGG
                     VVWAWRRLASPSRSRLGVPAEARVARRRDVAPLVVSSSEPPTGRLLLGRLAPNGPLLA
                     TEDRERHPMTGRRAAAIQGDRGSVALIGPTRAGKTVLASAGIIGWDGPVVALSVKRDL
                     YDATATARAARGELAVFDPGGVTRLPTARWTPLREVTTTSGALRAGRALAAAIPRQGV
                     TGGDFWATHGETLTSAYMCLAGLSQLLPGDSGGTVREPLTISRLASWAYMHVGITDPT
                     VNNLVRTGLTPRQPLEVQLLAKDAVTKLMAFEQEDPRIRASIYATARLAFDAWTESSV
                     AHSASLDPRDAYWSDELWDRTPRYVDLDWLMEGDNGRSNTLYLTAPATEFARLAPVLG
                     GLLGDLREQIHAWDIAGRRLAKPLLIVIDEAGQLELRWLPEEVSTIAGLGGMFVTGWQ
                     SRAQINHRYGSLADAVLGGHRSKIIFNGTDDLSTLEYVSKVAGTEHVAQRGWSADTGG
                     GRRTVSEHVQREDLLPAHVIRQMRRQEAVLLHGTLPPIHLRAVRWWEDRTLTALVPKR
                     DNGKPVAPPTTGTCPTRAGERGPVSPVLDPAVLAESVNSLPGPRNPSPGAQLPRSRPP
                     RSGPAPGQGQLRLEPSPTEPALVGRNRVVAYCDRCGTRLGIGAGRSERVGARDVVRCA
                     PACAS"
     gene            4844..5464
                     /locus_tag="PKY13_07530"
     CDS             4844..5464
                     /locus_tag="PKY13_07530"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOT79820.1"
                     /translation="MKSDLKKEIPAYQASKGRFDIVTVPTMRYLMIDGHGDPNTSVAY
                     ADALATLYPVAYKLKFWSKRELDRDYVVMPLEALWWSNDMDAFTIARDKSRWDWTAMI
                     MTPDWISHDAFDTVIKEVAASGDTPALDALRFQALDEGLCVQTLHVGAYDDEGPVLAA
                     MHDEFIPASELRMTGKHHEIYLSDPRRAAPDKLRTILRQPVTRSTN"
     gene            5727..8120
                     /locus_tag="PKY13_07535"
     CDS             5727..8120
                     /locus_tag="PKY13_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79821.1"
                     /translation="MAVQGQRIEFPTLQAMTAHLDRWAARRLGTPEEWSDALVGSRAW
                     LDYSARNQVLLASYGVDGPVAGPETWRLVPSTTEDRGCAVRAGEHGYPVRVPITTGGS
                     EPDPYLGGQRPTRAAVERWEWRPVFETGQLARKPAPGTLQPVTLPANLSGPTGDADLL
                     AAVRKVAGATIRGPLPRTNDPQQLLIDAAVRLRRSHKRPPLDPTLAAQVAHLVLDRVD
                     HHPTGGLAAFDPGALDSRERWERLQDVLEPARKLTAALGVAIGVDLTASPLPRMEIID
                     DRVVTSGRRHRLPAASLEQLPIGRWVTVGPYTATEWSARGEHAAGRGAFLRLNKSAYL
                     AAVETGDGATWRLEDIAARTGSGLLQTGTARTLDDARAEAVAAVRDRYPALTAPVTDT
                     GPSPVGAVEGWEQMPRAGRPVAAELWHLTGDITLYVLPAPGGRWQPAIQTPTTDGLEL
                     LCLQPSRNEAREHAELAGRRVARVAAISDPATSDRTLAAFANDGLYTRADLAHLIGGR
                     LTDHDAARIPGADAAELVELLGAAGFSPATTVAVLSADGIDAATAATVLPTAGVPIPD
                     GIRVLHERWHVPTTLAAELLDATASEMRTAGCTPVEIMAVRPRDVLRTLPDDPRMWEL
                     AAGSMATAGHPTSVIVDHLVAHAPSTDTFVTGMLTVVEPADALGVAAGRHAHADQLAA
                     VSEAAGLSPVETAVALAGTTDASIVLDVTTLRCDGDRNAAAEISGDVGIDPSSIASWL
                     EPAVASNVVALRPGLDLDTAALLAGLPAPGPALATDPTHGLDLLARSIDSTGLEPAQ"
     gene            8117..8467
                     /locus_tag="PKY13_07540"
     CDS             8117..8467
                     /locus_tag="PKY13_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79822.1"
                     /translation="MTDHPFTNTPHDDEPLDEGGTWLLDLVSAVDHLRGGYRPGLTVW
                     DALAEAAGWSADASGDGTPGATSFVLSREPARQAETQVAVRRWVTVMAERYNAGFQWP
                     HPLHRRSFPPDRLE"
     gene            complement(8514..8924)
                     /locus_tag="PKY13_07545"
     CDS             complement(8514..8924)
                     /locus_tag="PKY13_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79823.1"
                     /translation="MTTEQTDANPSTAGDRAEIDRLAERYIGVWSTADATRRDDLGSA
                     VYAENAEFFSSEDGDLRLIGRRSILENIGKVNERDVQGHGLVVRHVGTSVNHRVVKVS
                     WQMATAQGGVALEGVNLLVLDPAGKIAQDYIFIG"
     gene            9009..9602
                     /locus_tag="PKY13_07550"
     CDS             9009..9602
                     /locus_tag="PKY13_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT79824.1"
                     /translation="MSPPDGGPTRAAVVAAASALLESGGPDAVTLRAVGAAAGVSRST
                     PYRHFQDKADLLAALALQTLNDLGAAISEASSRGGDGALPRACFAYVRYAVEHSHHYM
                     LVFGDTPMAEPSAAIEEAADAGMGALGVLVERAQADGELGDGPPREMATMLWALLHGL
                     AQLQITGHLREPRTVQGDDGLRQLVTLALTALRPTQP"
     gene            complement(9642..>10653)
                     /locus_tag="PKY13_07555"
     CDS             complement(9642..>10653)
                     /locus_tag="PKY13_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79825.1"
                     /translation="RPLAALEKWAAHASAVDAAATQRLGHHPQVVRDRIARTRHTAEH
                     VAVGQRVKAWDRRNIGTVTALDDPAGTVHVTFVSANGSAARRSLGWGDITIVDPTTPT
                     PRVLDGDTRALVDSLVDRLDHQLENWTGHLAQHGVEPDDAHVYRSAASLAVDRNAAAL
                     QAAEPEWLLELLGARPTHDPAATQVWEDAVRDLAAARLRHAVISPAEPTRTDRSSPVW
                     ADAGSAVIDARQWLDTNRSIEPAKLVRRDSAELRERGSELDRLLATAPPDVRGLIDRI
                     IGGEALPFDDLDSVLYDALSTRDARRAWIVENWPYVVEAAEIATGLAADAAEMTIDEL
                     AL"
BASE COUNT         1602 a         3919 c         3532 g         1600 t
ORIGIN      
        1 gctgaacaca tcgagcatca cactcgattt cccaccaccc cgcggggctc tgtcccacca
       61 ggcgctcgga ccatggctcg agttctacaa tcaccgacga ccacacagcg ccctcggcca
      121 cacaaccccc gccagccgcc tcacggctga ctaacgctcc tgggatctac atctagcctt
      181 ggcgccgtgg gcgtcgtcac cagcgaggaa ctccgccaac gccagaaccc cgacggctcg
      241 ttcggccagc aagccacgcc gaccggtcgc gtcgtgacga cgatcttcgc ggcccggtcg
      301 ctccaagaag ccggattggt cgaccacccc agcctcgggc ccgcactcga cttcctcgcc
      361 cgaaccgcca cctgtggagg ttgcacgatc accggccaac gcgacggggt gttgtcctgt
      421 tacaccggga tgctcgcccg gctcatggtc cgcagccgtc ggctcgacga cgccgtgccg
      481 ctgctcgagt ggatcgtcac ctaccaaccc gtggtctacg gcgacaccac ctaccaccag
      541 ccgaccggac cgatatgggg cgagtatctg cggcaccgat acggcggctg catggccgcc
      601 acgacctgcc tactcggcct cgtccccgcc tgcggcgcgc tgctcgacgc acgcgacgcc
      661 ggcatcgacg ttcccgtcga cgcgcacctc gacgcgatgc ggcgactgct gatcgaccgt
      721 cgactcatgc accgcaccga tggaacgatc atcgcgctgt cgggccacac caaagccgac
      781 ccccacggca cccggtggat gctcccggcg tttccccgcg actacctcat cgacctcatc
      841 gaactcgtcg acctcgccca acgcctccac gttcccgaca gcgccatgac cgaggcagtg
      901 gacctgatcg cctcttggca gcttcccgac ggcagctggc cgacactcgc cagtcgacgc
      961 gtcgccgacg cctaccgacc cgacccggtc aaccggcacc gagcaagcgc catcaccact
     1021 gcacgggtcc acagcctcgg cctggacctg aacgaccggt gaacgagcga cccggcaatc
     1081 ggtcggcgcg cgcgtcgggc ccgcggcttc actgtcggag tgaccgtgct cgaaaccgga
     1141 cgattccgac tccgtccgcc gacttcccat gacgtaccgg ctctggtcga gcactggcga
     1201 gatggcgcag tacgcgagta cctgtgggac gacaagctcg tcgatgtcag ggtcgtggag
     1261 gccgtcgtcg ccgccagcga atccgaccac gcaacctccg gctggggcgg ttgggtcatc
     1321 cgaacccgcg acgaggaagc gccgctcgtt ggctggtgcg gcctgcgtgt cgggcctccc
     1381 gacggatccg cggcatcgga actgctcgtg gaactgacct acagcttggt gccgacgtgg
     1441 tggcgtacgg gcgccacagt ggaagcctca accgcagtgc tcgatcacgc tttcgcgaac
     1501 tcaaccctcg atgaggtgtt cggcggagtc gatgcgccca acctccgatc ggtggccacg
     1561 ttgcgacggc tcgggttcgt ccccgaccgc gaggtgcaac tcgatgtcgg tccgacgccc
     1621 tactggcggc tcggccgcgc tgactggctc gatcggcgac accggcccgc gtgaccggcc
     1681 ggcgctgtcg cgatccgccg ccgacgtggg tgggttcatg cggcgtggag gtactcatcg
     1741 accagaggcc ggagacgggc cgaggcccgc gacgcgagct tgcgctgcac cgcgctgcac
     1801 gctggcgtgg tggtgtcgag ggcgcggcgg attcggtggt cacggaacgc gtcccacagc
     1861 accggcgaca acgcgccggc atcgaccgcg tcagcgacgg cgttgccgaa gcgggcgagg
     1921 tcgactcggg ctgcgacgtc gtcggcgaga tcgtcgtggg cgaggtctcg tcggcgggcg
     1981 aggaggtcgg gatcggtggg ggtgatctcg acgaggttga cgacgccgcg atgcgtgacc
     2041 cggcgggcgg ccttgtggac gcgggtgtgg gcccggttga cgagccggcg ggcgaggtgg
     2101 gtgccgacgg tcggggcgtc aagcagcacg atggtcaact cggtcagcgc gtcgctgcgg
     2161 tattcgtcgg tgacggttcg cccgagccgg gctcgcagca gcggtaccag tgcgtaggcg
     2221 gcgacgacga ccgcccgctg atccctgccg gcgagttcga tgagccggcc gagcagttgg
     2281 tcgctgcagg ggctgtccgt gcggatcgtc tcgacgaggg cgtcgatcga ggggacgtcg
     2341 aggcgccagt gctcggggac accttgccgg cggaggtggc ggtattcggt gtcgagggtt
     2401 cggtaaaggt tcacggatcg ctccttgcat cggttgggat gcggcgagcg tccgaaacgg
     2461 gactcccggt ccaactcccg ggttcagtcc cggagttgct cccgacgcgg ttcgttcgct
     2521 cccggatgcg gccgtggcga tgaaccgaac tcccgaacgc gccgcccact cacccggcgc
     2581 cgacatcgcc ctcgtcgcca tcggcggcgt cctcatcgcc atcggcgcca tcacctgggg
     2641 cggcgcgggg ctcgcagcca ccgtcaccgg cggacagcta cacggcgggc tcgcggacgc
     2701 cttcggcgcc ctgggccgtc tcggttcgga cccgtcgcaa ccggccgccg catggggcaa
     2761 gaacgcgaca gggttgccgg gccccgtcgc ctactgggcg tgcaccgcac tcgtcgctgt
     2821 tgccgtcgcc gtgctcgccg gcggtgtcgt ctgggcgtgg cggcgattgg cgtcgccgtc
     2881 acggtcccgg ctcggggtgc cggccgaagc ccgcgtcgct cgccgtcggg acgtggcgcc
     2941 gttggtggtg tcgtcgtcag agccgccgac gggtcggctg ctgctcggcc ggctcgcccc
     3001 gaacggaccg ttgctggcca ccgaggaccg cgaacgccac cccatgaccg ggcgccgggc
     3061 cgcggcgatc caaggggatc gcggttcggt ggcgctgatc ggcccgaccc gggcgggcaa
     3121 gaccgtgctc gcctcggcgg ggatcatcgg ctgggacggc cccgtcgtcg ccctgtcggt
     3181 gaaacgcgac ctctacgacg ccaccgccac cgcccgcgcc gcacgaggcg aactagccgt
     3241 gttcgacccc ggcggcgtca cccgcctccc gaccgcccgc tggacaccac tacgggaggt
     3301 gaccaccacc tccggggcgc tacgcgccgg ccgggcattg gctgccgcga tccctcggca
     3361 gggggtgacc ggtggggact tctgggcgac ccatggcgag acgcttacct cggcctacat
     3421 gtgcctcgcc gggctctccc aactcctccc cggcgacagc ggcggcacgg tgcgggagcc
     3481 gttgacgatc agccggctgg cgtcgtgggc gtacatgcac gtcgggatca ccgaccccac
     3541 cgtcaacaac ctcgtccgca ccggcctcac tccccgccaa cccctcgaag tgcaactcct
     3601 cgccaaggac gcggtcacca agttgatggc cttcgaacag gaagacccgc gcatccgggc
     3661 cagcatctac gccaccgccc gcctcgcgtt cgacgcctgg accgaatcgt cggtcgccca
     3721 ctccgcgtcg ctcgacccac gcgacgccta ctggtccgac gagctctggg accgcacgcc
     3781 ccgctatgtc gatctcgact ggttgatgga aggcgacaac ggccgctcga acaccttgta
     3841 cctgacagcg ccggcgaccg agttcgcccg cctcgcaccc gtcctcggcg gcctcctcgg
     3901 tgaccttcgc gagcagatcc acgcctggga catcgccgga cgccgactcg ccaagccgtt
     3961 gctgatcgtg atcgacgaag ccggccaact ggagctgcgc tggctccccg aggaagtgtc
     4021 gaccatcgcc gggctcggcg ggatgttcgt caccggctgg caatcacgcg cccagatcaa
     4081 ccaccgctac gggtccctcg ccgacgccgt gctcggcggg caccgttcca agatcatctt
     4141 caatggcacc gacgacctct cgaccctcga gtacgtgtcc aaggtcgccg gcaccgaaca
     4201 cgtcgcccag cgaggatggt ccgccgacac cggcggtgga cgtcggactg tgtccgagca
     4261 cgtgcagcgc gaggacctgt tgccggcgca cgtgatccgt cagatgcgcc gacaggaagc
     4321 cgtcctcctc cacggcaccc tcccgcccat ccacctgcgc gccgtgcgct ggtgggaaga
     4381 ccgcaccctc accgcgctcg tgcccaaacg ggacaacgga aagccggtgg cgccacccac
     4441 gacggggaca tgtccgacgc gtgctggtga acgtggtccg gtgtcgccgg tgctcgaccc
     4501 ggcggtgctg gccgagtcgg tcaacagcct gcccgggcca cgcaacccgt cccctggcgc
     4561 tcagcttccg cgttcgaggc caccacggtc gggaccggcg ccggggcaag ggcagcttcg
     4621 actcgaaccg tcaccgaccg agccggccct ggtcgggcgg aaccgggtgg tcgcctactg
     4681 cgaccgatgc ggcacccgcc tcggtatcgg cgccggccgt tccgaacggg tcggcgcccg
     4741 agacgtcgtt cgctgtgcac ctgcgtgtgc ctcgtgatcc tcgtcggcag ccgacagggg
     4801 acctactgtc gaaggacatc tacccggcga gaggaggctt ggggtgaagt ccgatctgaa
     4861 gaaagagatc cccgcctacc aggcttcgaa gggccggttc gacatcgtga ccgtgccgac
     4921 gatgcgctac ctgatgatcg acgggcacgg cgatcccaac acctccgtcg cctacgccga
     4981 cgcgctcgcg accctgtacc cggtcgccta caagctgaag ttctggagca aacgtgaact
     5041 cgaccgcgac tacgtcgtga tgccgctcga agcgctctgg tggtcgaacg acatggacgc
     5101 cttcaccatc gctcgcgaca agtcccgctg ggattggacg gcgatgatca tgacgcccga
     5161 ctggatcagc cacgacgcct tcgacaccgt catcaaagag gtcgccgcca gcggcgacac
     5221 cccggcactc gacgcgctcc gcttccaagc actcgacgag gggctctgcg tgcagaccct
     5281 tcacgtcggc gcctacgacg acgaaggtcc ggtgctcgct gcgatgcacg acgagttcat
     5341 cccggccagc gagcttcgca tgacgggcaa gcaccacgag atctatctga gcgacccacg
     5401 acgagccgcg ccggacaagc tgcgcaccat cctgcgccaa cccgtcaccc gatcgacgaa
     5461 ctgaccccca tctacaggcg ggcgagagcg acgctgcggt cgatcaactc gaccagcacg
     5521 gcgtggtcga ggtcgtcgag gcgttcgatg tagttcgtca cgggtcgagg aactcggtca
     5581 cgctggcgtc ggtctgacga ctcttcggct cagccatcca tcgagggcca cgaaccggcg
     5641 aaaccgaccg acagcggcgt tcgggagcaa cttcgggagt tcggtcggga gcaactccgg
     5701 gagtgccgtt gcgcatcgtc cccgacatgg ctgtgcaggg tcagcgcatc gagttcccga
     5761 cattgcaggc gatgaccgcg cacctcgacc ggtgggccgc ccgccggctc gggacgcccg
     5821 aagagtggtc cgacgcgctg gtcgggagcc gggcatggtt ggactactcg gcccgcaatc
     5881 aggtgctcct cgcctcctac ggcgtcgacg gaccggtcgc tggccccgaa acctggcggc
     5941 tcgtcccgtc caccaccgag gaccgtgggt gcgcggtgcg cgccggggag cacggctacc
     6001 cggtacgggt gccgatcacc accggcggca gcgaacccga cccgtacctc ggcgggcaac
     6061 gccccactcg cgccgcggtg gagcggtggg agtggcgacc ggtgttcgag accgggcagc
     6121 tcgcccgcaa gcccgccccg ggcaccctgc aaccggtcac actgccagcg aatctgtctg
     6181 gcccgaccgg cgacgccgac ctgttggccg ccgtccgcaa ggtcgccggc gccaccatcc
     6241 gcggaccgct gcctcgcacc aacgaccccc aacagttgct tatcgacgca gcagtccggt
     6301 tgcggcgctc ccacaagcgg ccgccgctcg acccgaccct cgccgcccaa gtcgcccacc
     6361 tcgtcctcga ccgcgtcgac caccatccga ctggtggcct cgccgcgttc gaccccggcg
     6421 ccctcgattc gagggagcgg tgggagcggt tgcaggacgt cctggaaccg gcccgcaagc
     6481 tcaccgccgc cctcggggtc gccatcggcg tcgacctcac cgcctcgccg ctcccgcgga
     6541 tggagatcat cgacgaccgc gtcgtcacct ccggccgccg gcatcggctc cccgccgcgt
     6601 cgctcgagca acttcccatc gggcgatggg tcaccgtcgg cccctacacc gccaccgagt
     6661 ggtccgcccg tggggagcac gctgcggggc ggggtgcgtt cttgcggctg aacaagtccg
     6721 cctacctcgc ggcggtcgag actggtgacg gtgccacctg gcggctcgaa gacatcgccg
     6781 cccggactgg ctcggggctg ttgcagaccg gcaccgcccg caccctcgac gacgcccgcg
     6841 ccgaagccgt cgccgccgtc cgcgatcggt acccggcgct caccgcacca gtcaccgaca
     6901 ccggaccgtc gcctgtgggt gcggtcgaag ggtgggaaca gatgccgcgt gccggacgac
     6961 ccgtcgccgc ggagctgtgg cacctcaccg gcgacatcac cctctacgtc ctgcccgcac
     7021 ccggcggtcg ctggcaaccc gccatccaaa cccccaccac cgacggcctc gaactccttt
     7081 gcctccaacc gtcacgcaac gaggcacgag agcacgccga gctggccggg cggcgcgtcg
     7141 cccgcgtcgc tgcgatctcc gatccggcga cgtcggatcg gacactcgcc gcgttcgcca
     7201 acgacgggct ctacacccgc gcggatctcg cccacctcat cggcggacgg ctcaccgacc
     7261 acgacgccgc ccgcatcccc ggcgccgatg ccgccgaact ggtcgaactg ctcggtgccg
     7321 ccgggttcag ccccgccacc accgtcgcag tgctctcagc cgacgggatc gacgccgcca
     7381 ccgccgccac cgtcctaccg acagctgggg tgccgatccc tgacgggata cgggtgctgc
     7441 acgaacgctg gcacgtcccc accaccctcg ccgccgaact cctcgacgcc accgccagcg
     7501 agatgcgcac cgccggctgc acccccgtcg agatcatggc cgtccgaccc cgcgacgtcc
     7561 tgcgaaccct gcccgacgac ccacgcatgt gggagctcgc cgccgggtcc atggccaccg
     7621 ccggacaccc gacctcggtc atcgtcgatc acctcgtcgc ccacgccccc agcaccgaca
     7681 cgttcgtcac cggaatgttg accgtcgtcg aacccgccga tgcccttggc gtcgcggcag
     7741 gccgtcacgc gcatgccgac caactcgcgg cggtgtccga agccgccggc ctctcacccg
     7801 tcgagaccgc cgtggcgctc gccggcacca ccgacgcgtc gatcgtgctc gacgtcacca
     7861 cgttgcgttg cgacggcgac cgcaacgctg cggcagagat atccggcgat gtcggcatcg
     7921 acccgtcgtc gatcgcgtcg tggctcgaac cagcagtggc gtcgaacgtc gtcgcgctcc
     7981 gccccggcct ggatctcgac acggccgcgc tgctcgccgg gcttcccgcg cctggaccgg
     8041 cgctcgccac cgacccgacc cacggcctcg acctcctcgc tcgcagcatc gattccaccg
     8101 gactggaacc cgctcaatga ccgaccaccc cttcaccaac acgccgcacg acgacgagcc
     8161 gctcgacgaa ggcggcacct ggctgctcga cctcgtctcg gccgtcgacc atctccgtgg
     8221 cggctaccgc ccgggtctca ccgtctggga tgcgctcgcc gaagctgcgg ggtggtcggc
     8281 cgatgcctct ggcgacggca ccccaggagc cacctcgttc gtcctgtccc gagagcctgc
     8341 acgccaagcc gagacgcagg tcgcggtgcg gcgatgggtg acagtgatgg cggagcggta
     8401 caacgccgga ttccagtggc cgcatccgct ccatcgccga tcattcccgc cggaccgcct
     8461 ggagtagagc gtggcggcgg ctgagtcgct gtccctctgg cgctgtgttg gcgctagccg
     8521 atgaagatgt agtcctgtgc gatcttgccg gcggggtcga gcacgagcag gttcacccct
     8581 tccaaggcga cgccgccttg tgcggtcgcc atctgccagc tcaccttcac gacgcgatgg
     8641 ttcaccgagg taccgacgtg gcgcaccacc aacccgtgac cttggacgtc gcgctcgttg
     8701 actttcccga tgttctccag gatgctcctc cgcccgatca ggcgaaggtc cccatcttcg
     8761 gaggagaaga actcggcgtt ctcggcgtag accgccgacc ccagatcatc acgccgggtc
     8821 gcgtcggcgg ttgaccacac gccgatgtag cgctcggcca gcctgtcgat ctcggctcgg
     8881 tcaccggcgg tgctggggtt ggcgtcggtc tgttcggtcg tcatgttgtg ctcctgctcg
     8941 tttgttgaca gtgtcagcat agtcggattg cggacactgt caacagtcgt cgggtatcgt
     9001 ttgagcccat gagcccgccc gacggtggtc cgacccgcgc cgcggtggtc gccgcggcca
     9061 gcgcgctgct cgagtccggc ggccccgacg cggtcacgct gcgagccgtg ggcgcggctg
     9121 ccggggtgtc tcgatcgacc ccgtatcgac acttccagga caaggccgat ctgctcgcgg
     9181 cgctcgcgct gcaaacgctg aacgatctcg gggcggcgat cagcgaggcc agcagccgcg
     9241 gcggggatgg cgcgttacct cgcgcctgtt tcgcgtacgt gcgctacgcc gtggagcact
     9301 cgcatcacta catgctggtc ttcggtgaca cgccgatggc cgaaccttcg gccgccatcg
     9361 aggaagccgc cgacgccggt atgggggcgc tcggggtgct cgtcgaacga gcacaggcag
     9421 acggcgagct cggcgacggg ccaccacgcg agatggccac gatgctgtgg gcgctgctcc
     9481 atggcctggc tcagctgcaa atcaccggac atctgcgaga gccccgaacg gtgcaaggcg
     9541 acgacggcct ccggcaactc gtgaccttgg cgctcaccgc gcttcggccc acccaacctt
     9601 gacgccaacc gtgcgggaga ccacccgccc gagcgcgctg ttcacaacgc caactcgtcg
     9661 atggtcatct cggccgcatc tgcagcaagt cccgtggcga tctcggcggc ttcgacgacg
     9721 tagggccagt tctcgacgat ccatgcccgt cgagcgtccc gcgtggacag ggcgtcgtac
     9781 aggaccgagt cgagatcgtc gaagggcaac gcctcgccac cgatgatcct gtcgatgagt
     9841 cctcgcacgt ccggtggtgc ggtggcgagg agccggtcga gctcactgcc gcgctcccgc
     9901 aactctgcgc tgtcccgccg tacgagcttc gcgggttcga tgctgcggtt ggtgtcgagc
     9961 cattggcggg cgtcgatcac ggccgagccg gcgtcggccc ataccggcga cgaccggtct
    10021 gtgcgggtcg gctcggcggg gctgatcacg gcgtgacgaa gtcgggcggc ggcaaggtcg
    10081 cgcacggcgt cctcccacac ctgggtggcc gccgggtcgt gggtggggcg ggcgccaagc
    10141 agttcgagga gccactccgg ctccgccgct tggagagcag cggcgttgcg atcgacggcg
    10201 agcgatgcgg cggaacggta gacgtgggcg tcgtcgggtt ccaccccgtg ttgggcgagg
    10261 tggccggtcc agttctcgag ctggtgatcg aggcgatcga cgagggagtc gacgagcgcc
    10321 cgggtgtcac cgtcgaggac tcgtggcgtc ggtgtggtgg ggtccacgat ggtgatgtcg
    10381 ccccatccga gcgaccgacg ggccgcgctg ccattggcgg acacgaacgt gacatggacg
    10441 gtgccggcgg gatcgtcgag ggcggtgacc gtgccgatgt tacgacggtc ccacgccttg
    10501 acccgctgcc cgacggcgac gtgctcggcg gtgtgacggg tccgggcgat ccggtcccgc
    10561 actacttgtg gatggtggcc gaggcgttgg gtggcggcgg cgtcgactgc tgaggcgtgg
    10621 gcggcccact tctcgagcgc cgccagcggg cgg
//
LOCUS       DAOSOA010000089        10568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6273_length_10568_cov_5.895082, whole genome shotgun sequence.
ACCESSION   DAOSOA010000089 DAOSOA010000000
VERSION     DAOSOA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6273_length_10568_cov_5.895082"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKY13_07560"
     CDS             <1..678
                     /locus_tag="PKY13_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HOT79826.1"
                     /translation="THGTPAVASTSSFERVELDAHSWVDVCRGFLIGADEVMTELIEG
                     VAWRTGRRQMYDREVDDPRLSRWWRDPNDVPHPVLGAARASICAHYDRELGGVGLNYY
                     RDGADSVAFHRDRELRDPATTLVAIVTLGSQRPFRLRPFGGGPSIDLGPASGDLLVMG
                     GRCQTEWEHGVPKVGRSVGPRVSASWRWSPDGSHRRAQRDGYFTSRQWRAAAPGSAET
                     GPVHPFG"
     gene            792..1298
                     /locus_tag="PKY13_07565"
     CDS             792..1298
                     /locus_tag="PKY13_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79827.1"
                     /translation="MRNKKKLVGAALAVAAIAFTGAASTASNTVAAGAGRLGFSDTIV
                     TGGTINAINYTLDNTMPGKVIAVKINVDDLTPAAVVRVQLSAEAPVNTDPDILTEVIS
                     CVRGLETLAPVPDDPGTAGVDESVPGKYDYDCTVPTTPDDPGTVGVDEADPVIPADKL
                     EHTYVAIS"
     gene            1488..3581
                     /locus_tag="PKY13_07570"
     CDS             1488..3581
                     /locus_tag="PKY13_07570"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT79828.1"
                     /translation="MRRTRGGARRWTRWSTFALILAVAVALFLRSNVALLSTQGTSMA
                     PRFHTGDLAITVAASNYAIGDIVAYKAQPGGQVVLHRIIEKAGDEYRFKGDNNSWVDP
                     VKPTTKDLVGKLWLRVPRAGALVNVRSRFWQIALLVVAALLIFGGGAAHRTRRRRAET
                     ETEAETNPPRPRRPVSTTGDVIAALTYPGIAVAVTLLLAVLAFQRPASSQTTAAREYT
                     QQVRYGYTASAPSGTTYPDGLVTTGSPIFLKLVSSLKFQLTYDVTAKSAMVHNAGGTY
                     SIDLVVDGPVSWRRTIPLQPTTPFTGQSFSSEVAVDIPQIREIVAANAAETGVKGDAI
                     SVRIVPTVNANGFIDGAPFATTFSKSLTMDIGPTTVTLSTQGKSAAPTAMSEGGDMSQ
                     TAPSTAATSTAGPTPDQLTSLQTGEYRADITKPNRFELFSRRLDVSRARLMTGAALVM
                     ALIWMAVAAATQDRLRHRSESTRITHRYREHIVEVTSLSPADQVVDVATIAALVKIAD
                     QSGRFMLHLTGGPADLFVVDDDGTRYRYVSGSPPTASTGPPEPARESPPPPIALLEPQ
                     LEPEPVLDTAPVLETEPEPELVPEPDPEPESEPQPAAGLIVASDAPATDAVFRALLGS
                     NGFFDSEPAHDPGTPPPFVPAPASHDAGSPPPFFGVAPAGPPPPASLDHAIRLTPLTQ
                     AERDELGIDESINRP"
     gene            complement(3568..4317)
                     /locus_tag="PKY13_07575"
     CDS             complement(3568..4317)
                     /locus_tag="PKY13_07575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT79829.1"
                     /translation="MRFEGRSAVVTGAASGLGRSVAQQLAAEGAKVYGVDLNEAGLAE
                     TQGLVQAAGGTMSFGVGDISDRMTAHGLIANEMDEFGQLDVLINCAGVLRAGHAVDFT
                     EADYDLVFNVNVRGTFWMCQAAIPHLLDSRGNIVNVASNAGLMGQAYTAVYAASKAAV
                     VNLTRSLAMEFVKSKIRINAVAPGGIETPLVKSASFPDDTDWALVQPFMGYRKMSKPE
                     EIAEVVLFVASPAASAMHGAIVSADTGITAG"
     gene            4383..5186
                     /locus_tag="PKY13_07580"
     CDS             4383..5186
                     /locus_tag="PKY13_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79830.1"
                     /translation="MDDYPVKLGAMLYTLVDPERGHEVAYNRWYERDHFYGGCMIGPG
                     ILAGSRWVATKPYKDLRFPADSGVAIPTEAGSYLATYFVQKGHEAEHFAWTSPQVFEL
                     YKEGRGFEERIHAHTALYTHQKPFYRDDDGVPVELALDHRFAGLVSVHVDRAEGVKHP
                     QFDEWFEAEGAPLLLGADSPVAIACSWRPIIPREGEGASPMKLGSGPGTLQRTMQLFF
                     LDSEPAQHWQRFVDYAAAVDASGLATVTLAAPFIPTIPGTDTYADQLWE"
     gene            complement(5250..5954)
                     /locus_tag="PKY13_07585"
     CDS             complement(5250..5954)
                     /locus_tag="PKY13_07585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOT79831.1"
                     /translation="MTDDSGAAPGDDASFMTFGRRRSAGQAYLVRPGEDGEGTGPGVL
                     VLSSWWGLTRGIKDFCNRLCDEGFVVLAPDLMGGALPETAAEAELELGETDPNTTANL
                     VLSSAVALRSATDEPAGPVAVIGFSMGASWALWAATRQPESFHKVVAYYGSQTIDFAD
                     MAASVQGHFAENDDLVSADDVVLLEADLFELGRDPEMWHYADTRHWFAEAGVDGHYDE
                     AAAELAWQRTLDFLKD"
     gene            6022..6819
                     /locus_tag="PKY13_07590"
     CDS             6022..6819
                     /locus_tag="PKY13_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOT79832.1"
                     /translation="MSRRLLDRPEIVSHDIVTRAMHEAKSDDFGGMSWSEPLEVLTRS
                     LDREASLTMSGRRAARDRLVARLLDRRSIRDVTSDGTGAVGTVVVTGPDPDSVRSGEQ
                     ALGGSGIDGITLLESTFTSMSFEVDAHIPSYAEWLTEADLHAPYADVARRCTHRVLGA
                     MDFAERTAEIDRALPGALLVEIDRPLEDAVEAITHHSVLGRRHDSRTVDPAKVERYWR
                     WRLGLRQERRAGARPADLTVGYDELVAAPAAFAAAVASAVGGSSPPR"
     gene            complement(6738..8213)
                     /locus_tag="PKY13_07595"
     CDS             complement(6738..8213)
                     /locus_tag="PKY13_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT79833.1"
                     /translation="MTSTIDTTAERRSGNNALAAALVCLVTVVAFETMSVATIMPKVL
                     ADIGGLGLYGWAFSGLALGEVMGIVIAGTWTDRVNPVRPILVGLVVYSVGLVISGLAN
                     DMLVVVIGRVLQGYGSGTVPAVAYVCVGRGFAEAERPRVFAWMSTAWVVPSMIGPSAA
                     GVLEKHVGWRAVFLGLIPVVICVGALAIRPIARLGNPVAGATATPDDGAVGSPARTVR
                     LATVAVLGTGLALGAFQLRNVVALSGLFVLGAVTLVWAFRRIAPAGTLRLARGVPSTV
                     AVRGMLTFAFLGADAYVSLALQDVKGMSATSAGIVLSSAALTWTAGSWYSAKRITRVG
                     PRRLVTAGLAAVVVGIAAMVLVVNSSVNSWWAIGAWLVGGLGIGLAYAPLTQAVISAA
                     DPAQLGAATSALQLSDVLGFALGTGLGGAIIALADRHGATVDGSSVHAGVLLVFGVTA
                     AVGAVGVASARRMHRYLGGDEPPTADATAAANAAGAATSSS"
     gene            complement(8245..8829)
                     /locus_tag="PKY13_07600"
     CDS             complement(8245..8829)
                     /locus_tag="PKY13_07600"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOT79834.1"
                     /translation="MAIELPALPYAQDALAPKGISAETLEYHYGKHHQTYVTVLNGLI
                     EGTDLADSSLEEIILAADAGPLFNNSAQVWNHTFYWNSMSPTGGGEPTGELADKINSA
                     FGSLDGFKEQFAQAGTTQFGSGWAWLIEGDSGLEITKTANADLPLKHGQKALLTMDVW
                     EHAYYIDFRNARPNYIKNFLDNLINWDFAAANLG"
     gene            complement(8932..9684)
                     /locus_tag="PKY13_07605"
     CDS             complement(8932..9684)
                     /locus_tag="PKY13_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79835.1"
                     /translation="MVDAGATDHTAGEPSWSLTIALAAVIVVALLAGIVALTDGSPST
                     VAGAGAGTDRGPASPSSAPTPSTAASTSTSTSAASTSSTTTSSTESSSPPSTEPVAPT
                     TTGAPGTVVGGFAVSFPTPPRVASVPVATASGTVTRTEYDLDGPDGSLISVTSVESAL
                     RGRPVTSVLADVAAQAGRGLGAAPIVGTATTVGGQPALPFRVSTSDGVVRAVVVHGAD
                     RLTTVSMFTPGHGPAVAADRVFDELVASVRLV"
     gene            complement(9696..10052)
                     /locus_tag="PKY13_07610"
     CDS             complement(9696..10052)
                     /locus_tag="PKY13_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOT79836.1"
                     /translation="MADVTVYHNPNCNSSKTALAMAADAGVDVDVVLYLKTKPDAEEI
                     ASIVEKLDGPVTELVRRDANFAQLGLTDADVQTAEQVIPLLAANPKLLQRPIVVKGDR
                     AVVGRPKERIADLLGS"
     gene            10171..>10568
                     /locus_tag="PKY13_07615"
     CDS             10171..>10568
                     /locus_tag="PKY13_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79837.1"
                     /translation="MNQEPAISQHELRSPASVDLAGIPLSMIEDILLRTTIAEGRPSM
                     VRVAERLHCGVGVIESVVSSLRDRRLMEYEGMQGRTYLLSVTEAGRAHSRQRSAECRY
                     AGPLPVPLDLYQRVVRAQRPNVHLDRDQLT"
BASE COUNT         1691 a         3771 c         3567 g         1539 t
ORIGIN      
        1 acgcacggca cgccggcagt ggcgtccacc tcgtcgttcg agcgcgtcga gctcgacgct
       61 cactcgtggg tcgacgtgtg ccgagggttc ctgatcggcg ccgacgaggt gatgacggag
      121 ctgatcgagg gcgtcgcgtg gcgcaccggc cgtcggcaga tgtacgaccg cgaggtcgac
      181 gacccccggc tcagccgctg gtggcgcgac cccaacgacg ttccccaccc agtcctcggg
      241 gcggcacggg catcgatctg tgcccactac gaccgcgagc tcggcggcgt ggggctcaac
      301 tactaccgcg acggcgccga cagcgtcgcg ttccaccgtg accgagagct gcgcgatccg
      361 gcgaccaccc tcgtcgcgat cgtgaccctc gggtcgcaac gcccgttccg gctgcgaccg
      421 ttcggcgggg gcccgtcgat cgacctcggt ccggcgtcgg gcgatctgct ggtgatgggg
      481 ggccgctgcc agaccgaatg ggagcacggc gttcccaagg tcggccggag cgtcggccca
      541 cgcgtgtcgg cgtcatggcg atggtcgccc gacggcagtc atcgccgcgc ccagcgcgac
      601 ggctacttca cgtcgcgcca gtggcgcgcc gcggctcccg gttccgccga aaccggcccg
      661 gttcacccgt tcgggtagtc cgttcggctc ttcatggctg agaactcctc tgatcggtaa
      721 aggcaatgcc cccatccgcc gatcacttct ccgagccagg acggctgttc agaaccaaag
      781 gagtgaccct catgcgtaac aagaagaagt tggtcggcgc cgccctcgct gtggctgcaa
      841 ttgccttcac cggcgccgcg tcgaccgcga gcaacaccgt ggccgccgga gccggccgcc
      901 tcggtttcag cgacacgatc gtgaccggcg gcaccatcaa cgccatcaac tacacgctcg
      961 acaacacgat gcccggcaag gtcatcgccg tgaagatcaa cgtcgatgat ctcacgccgg
     1021 ccgccgtggt gcgcgtccag ctgagcgccg aggccccggt caacaccgac cccgacatcc
     1081 tcaccgaggt catcagctgc gtgcgtggtc tcgagaccct cgcccccgtc cccgatgatc
     1141 ccggcaccgc cggtgtcgac gagtcggttc ccggcaagta cgactacgac tgcaccgtgc
     1201 cgacgacgcc ggacgacccc ggtaccgtcg gtgtcgacga ggccgatccg gtcatccccg
     1261 ccgacaagct cgagcacacc tacgtggcca tcagctagtc gatcatcact cccgaatggg
     1321 caggccggtt ctccggcctg cccattcggc gttttcgggc cggttcggac gatcccggaa
     1381 ccgcaaacga cccaggatcg tgtgcccgga gaccccccag aacagctcaa cttcggggat
     1441 cccgggccga tcggaccagt gcgatatcga cagttcgagg tggagagatg cggagaacga
     1501 ggggaggcgc gcggcgatgg acccgatggt cgacgtttgc gctcatcctc gcggtcgccg
     1561 ttgccctctt cctgcgctcg aacgtggccc tgctctcgac acagggaacg agcatggcgc
     1621 cccggttcca caccggtgac ctggccatca ccgtcgcggc atcgaactac gcgatcggtg
     1681 acatcgtggc ctacaaggcc cagcccggcg gacaggtggt cctgcaccgc atcatcgaga
     1741 aggcgggcga cgagtaccgg ttcaagggcg acaacaactc ctgggtcgac ccggtcaaac
     1801 ccacgacgaa ggatctcgtc gggaagttgt ggctccgggt cccccgcgcc ggcgcgttgg
     1861 tgaatgtgcg gagcaggttc tggcagatcg ccctgctggt cgtcgccgcg ctgctgatct
     1921 tcggtggcgg cgcggcgcac cgcacgcggc gacgacgggc cgagaccgag accgaggccg
     1981 agaccaaccc gccccgcccg cgacggccgg tgagcacgac cggcgacgtg atcgcagcgt
     2041 tgacctatcc gggcatcgcc gtcgccgtga ccctcctgct cgcagtcctc gcgttccagc
     2101 ggccggcgag cagccagacc accgccgccc gcgagtacac ccaacaggtc cgctacggct
     2161 acacggcgtc cgccccgtcg ggcaccacct atcccgatgg cctcgtcacc accggatcgc
     2221 cgatcttcct gaagctcgtc tcgtcgctga agttccaact cacgtacgac gtgacggcga
     2281 agtccgccat ggtgcacaac gccggcggca cctactcgat cgacctggtc gtcgacggcc
     2341 cggtgtcgtg gcgccggacg atcccactcc agccgaccac gccgttcacg ggccagagct
     2401 tctcgtccga ggtcgccgtc gacatccccc agatccgcga gatcgttgcc gcgaacgctg
     2461 cggagaccgg ggtgaagggc gatgcgatct cggtgcggat cgtgccgacc gtcaacgcca
     2521 acgggttcat cgacggggca ccgttcgcca cgacattctc caagagcctc acgatggaca
     2581 tcgggccgac gaccgtgacc ctgtcgacgc agggcaagag cgccgccccc accgcgatgt
     2641 ccgagggtgg cgacatgtcg cagacggctc cgagcaccgc cgcgacctcc acggccgggc
     2701 cgacgcccga ccagctcacg tcgctccaga cgggcgaata ccgcgccgac atcaccaagc
     2761 cgaaccgctt cgagctgttc tcccgtcgcc tcgacgtgtc ccgggcccgg ctcatgaccg
     2821 gggccgcgct ggtcatggcg ttgatctgga tggcggtcgc cgctgcgacg caggaccgcc
     2881 tgcggcaccg ctccgagtcg acccgcatca cccaccgcta ccgcgaacac atcgtcgagg
     2941 tgacgtcgct gtcccccgcc gaccaggtgg tcgatgtcgc cacgatcgcc gccctggtca
     3001 agatcgccga ccagagcggt cggttcatgc tgcatctcac cggggggccg gccgacctct
     3061 tcgtcgtgga cgacgacggc acgcgctacc ggtacgtgag cgggtcaccg ccgactgcgt
     3121 cgaccggccc acccgagcct gcgagggagt cgcccccacc gccgatcgct ctcctcgagc
     3181 cgcaactcga gccggagccc gtgctcgaca cggcgcccgt gctcgaaacg gaacccgagc
     3241 ccgaactcgt cccagaacca gaccccgaac ccgagtccga accgcagcct gcggccgggc
     3301 tcatcgtcgc gtccgacgct ccagcgaccg acgcggtgtt ccgtgccctg ctcggaagca
     3361 acggattctt cgacagcgaa cccgcacacg accccgggac accacccccg ttcgtccccg
     3421 cgccggcgtc gcacgatgcc gggagtcctc cgccgttctt cggggtcgcc ccggccggcc
     3481 cgccgccgcc ggcgagcctc gatcacgcga tccggctgac gcccctcacc caggccgagc
     3541 gggacgagct cgggatcgac gagtcgatca accggccgtg atgccggtgt cggcgctgac
     3601 gatcgcgccg tgcatcgccg acgccgccgg cgacgccacg aacaggacga cctcggcgat
     3661 ctcctcgggc ttcgacatct tgcggtatcc catgaacggc tggacgagcg cccaatcggt
     3721 gtcgtcgggg aagctcgccg acttgaccaa cggcgtctcg atgccgccgg gggccacagc
     3781 gttgatgcgg atcttcgact tcacgaactc catcgccagc gagcgggtga ggttgacgac
     3841 cgctgccttc gacgccgcgt agaccgcggt gtacgcctgg cccatcaggc cggcgttgga
     3901 ggcgacgttg acgatgttgc cgcgggagtc gagcaggtgc ggaattgccg cctggcacat
     3961 ccagaacgtg ccgcggacgt tgacgttgaa cacgaggtcg tagtcggcct cggtgaagtc
     4021 gacggcgtgg ccggcgcgca acacaccggc acagttgatg aggacgtcga gctggccgaa
     4081 ctcgtccatc tcgttggcga tcagaccgtg ggcggtcatg cggtcggaga tgtcaccgac
     4141 gccgaacgac atcgtgccgc cggcagcctg cacgagcccc tgggtctcgg caagaccggc
     4201 ctcgttgagg tcgacgccgt agaccttggc gccttcggcc gcgagctgct gggccaccga
     4261 gcgaccgagc cccgatgccg cgcccgtcac cactgctgat cgaccttcga accgcatacc
     4321 gactcccctt tggaatgccc gtcggcctga gaatctgacg gagcgtcagg tacggtagcc
     4381 gcatggatga ctaccccgtg aaactcggtg cgatgctcta caccctcgtc gaccccgagc
     4441 ggggccacga ggtggcctac aaccgctggt acgaacggga tcacttctac ggcggctgca
     4501 tgatcggccc cggcatcctc gccggcagcc ggtgggtcgc gaccaagccg tacaaggacc
     4561 ttcgtttccc cgccgactcc ggcgtcgcca tcccgaccga ggccggcagc tacctggcca
     4621 cctacttcgt acagaagggc cacgaggccg agcacttcgc gtggacgtca ccgcaggtct
     4681 tcgagctcta caaggaaggc cgcgggttcg aggagcgcat ccacgcccac acggcgctct
     4741 acacccacca gaagccgttc taccgtgatg acgacggcgt gccggtcgag ctcgcgctcg
     4801 accaccgctt cgccggcctc gtgtcggtgc acgtcgaccg tgccgagggt gtgaagcacc
     4861 cgcagttcga cgaatggttc gaggccgagg gtgcaccgct gttgctgggc gcggattccc
     4921 ccgttgccat tgcctgctcg tggcgcccga tcatccctcg tgagggcgaa ggcgcgtccc
     4981 ccatgaagct cggctcgggc cccggcaccc tgcagcgcac gatgcagctg ttcttcctcg
     5041 actccgagcc cgcgcagcac tggcagcgct tcgtcgacta cgccgccgcc gtcgacgcct
     5101 cgggcctcgc aacggtgacg ctcgccgcgc cgttcatccc gacgatcccg ggtacggaca
     5161 cgtacgcgga ccagctctgg gagtaagccc tcgtccaact tcaggctcag ccgtgcggat
     5221 gtgcccgggt gagcctgaag ttcgagcggt cagtccttga ggaagtcgag cgtgcgctgc
     5281 cacgccagtt cggcggcagc ttcgtcgtag tgcccgtcga cccctgcctc ggcgaaccaa
     5341 tggcgggtgt cggcgtagtg ccacatctcg gggtcgcgac ccagctcgaa cagatcggcc
     5401 tcgaggagca caacatcgtc agcgctcacg aggtcgtcgt tctctgcaaa gtggccctgc
     5461 acactcgcgg ccatgtcggc gaagtcgatc gtctggctgc cgtagtaggc aacgaccttg
     5521 tggaacgact ccggctgacg ggttgcggcc cacaacgccc aggacgctcc catcgagaat
     5581 ccgatcacgg cgaccggccc ggcgggttcg tcggtcgcgg accggagcgc caccgcgctc
     5641 gacagcacga ggttggccgt ggtgttcggg tcggtctcgc ccaactcgag ctcggcctcc
     5701 gcggcggtct cggggagggc gccgcccatc aggtcgggtg ccagcaccac gaacccctcg
     5761 tcacacaggc ggttgcagaa atccttgatg ccgcgggtga gcccccacca cgacgacagc
     5821 accagcacgc cggggccggt gccctcgccg tcctcgccag gccgcacgag ataggcctgg
     5881 ccggcgctcc gccggcggcc gaaggtcatg aaggatgcgt cgtcgccggg ggcggcgcct
     5941 gaatcgtcgg tcacggcgcc cagcctgcca cgccgatcgg gcctcgcccg ccgcagttcc
     6001 gcaccggtat ggtcgtcgcc gatgtcacga cgcctgctcg accgtcccga gatcgtgtcc
     6061 catgacatcg tgacccgggc catgcacgag gccaagagcg acgacttcgg cggcatgtcg
     6121 tggtccgagc cgctcgaggt gctgacgcgg tccctcgacc gggaggcgag cctcaccatg
     6181 tccgggcgcc gcgccgcccg cgaccggctg gtcgcccggc tgctcgatcg gcgttcgatt
     6241 cgcgacgtca cctccgacgg aaccggggcc gtcgggaccg tcgtcgtcac gggccccgac
     6301 cccgactccg ttcgatccgg cgagcaggcg ctcggtgggt ccgggatcga cggcatcacg
     6361 ctgctcgaat cgacgttcac gtcgatgagc ttcgaggtcg acgctcacat cccgtcctat
     6421 gccgagtggc tcaccgaggc cgacctccac gctccctacg ccgacgtggc tcgccggtgc
     6481 acccaccgcg tgctgggtgc catggacttc gcggaacgga ccgctgagat cgaccgtgct
     6541 cttcccggcg cgttgctcgt cgagatcgac cgacccctcg aggacgccgt cgaggcgatc
     6601 acccaccaca gcgtgctcgg gcgccgccac gacagccgca ccgtcgaccc ggcgaaggtc
     6661 gagcggtact ggcggtggcg gctggggctg cggcaggaac ggcgagccgg cgctcgaccc
     6721 gccgacctga cggtcggcta cgacgagctg gtcgcggcac ctgcggcgtt cgctgcggct
     6781 gtggcgtcgg cggtcggagg ctcgtcccca cccaggtagc ggtgcatccg ccgcgccgat
     6841 gccacgccga ccgcgccgac cgctgccgtc acgccgaaca cgagcagaac accggcatgc
     6901 acggaggagc cgtcgacggt cgccccgtgg cgatcggcca gggcgatgat cgccccaccc
     6961 agcccggtac cgagcgcaaa gcccaagacg tcgctcagct gcagcgccga cgtcgccgcg
     7021 cccaactggg ccgggtcggc ggcagagatg acggcctggg tcagcggagc gtacgcgaga
     7081 ccgatcccga gccctccgac cagccaggcg ccgatcgccc accaggagtt gaccgaggag
     7141 ttgaccacca ggaccatcgc cgcgatgccg acgaccaccg ccgcgagacc ggcggtgacg
     7201 agacggcggg ggccaacccg ggtgattcgc ttcgcggagt accacgagcc ggcggtccac
     7261 gtcagcgccg ccgacgagag gacgatgccg gcgctcgtgg ccgacatgcc cttgacgtcc
     7321 tgcaatgcga gcgacacgta ggcgtcggcg cccaggaacg cgaacgtcaa catgccccgc
     7381 accgcgacgg tcgacggcac gcctcgcgcc agtcggagcg tgccggccgg agcgatccgg
     7441 cggaacgccc acaccagggt gacggcgccg agcacgaaca acccactgag cgccaccacg
     7501 ttgcggagct ggaacgcgcc gagcgcgagg ccggtgccga gcacggccac ggtggccaat
     7561 cggacggtgc gcgccggcga accgacagcg ccgtcgtccg gcgtggccgt ggcgccggcg
     7621 accgggttgc cgaggcgcgc gatcgggcgg atcgcaagtg cgccgacgca gatcacgacg
     7681 gggatgagcc cgaggaacac cgcccgccac ccgacgtgct tctcgagcac accggccgcg
     7741 ctcggcccga tcatcgacgg cacgacccat gcagtcgaca tccacgcgaa cacccgcggc
     7801 cgttcggcct cggcgaagcc gcgaccgacg cacacgtacg cgacggcggg aaccgtgccg
     7861 gagccgtagc cctgcaggac gcggccgatc accacgacga gcatgtcgtt ggccaggccc
     7921 gagatcacca gaccgaccga gtacacaacg aggcccacga ggatcggtcg caccgggttc
     7981 acgcggtcgg tccacgtgcc ggcgatcacg atgcccatga cctcgccgag ggcgagaccg
     8041 gagaaggccc agccgtacag gccgaggccc ccgatgtcgg cgagcacctt cggcatgatc
     8101 gtcgcgaccg acatcgtctc gaacgcgacg acggtgacga gacagacgag cgccgcagcg
     8161 agggcgttgt tcccgctgcg gcgctctgcc gttgtgtcga tggtggaggt caccgcgggt
     8221 gcggtgtgcc ggcgaccgtt ctcactaacc caggttcgcg gcggcgaagt cccagttgat
     8281 gaggttgtcg aggaagttct tgatgtagtt cggccgcgcg ttgcggaagt cgatgtagta
     8341 cgcgtgctcc cacacgtcca tcgtgagcag ggccttctgg ccgtgcttga ggggcagatc
     8401 ggcgttggcc gtcttggtga tctcgaggcc ggagtcgccc tcgatcagcc aggcccaacc
     8461 cgaaccgaac tgggtggtgc ccgcctgggc gaactgctcc ttgaagccgt cgaggctgcc
     8521 gaacgccgag ttgatcttgt cggccagctc gccggtgggc tcaccgccgc cggtgggcga
     8581 catggagttc cagtagaagg tgtggttcca cacctgggcg gagttgttga agaggggccc
     8641 ggcgtcggca gcgaggatga tctcctcgag cgacgagtcg gcgaggtcgg tgccctcgat
     8701 caggccgttc aggaccgtca cgtaggtctg gtggtgcttg ccgtagtggt actcgagcgt
     8761 ctcggcggag atgcccttgg gtgccagcgc gtcttgggcg tagggcagtg cgggaagttc
     8821 aatggccatg gtttgtcatc cttgtgcggg gagcggttcg ggagctccgt ggccaaacta
     8881 cccgatgacg agccgcctca ttccccgtcc gaggggcagt tcggcccgtg ttcagacgag
     8941 gcggaccgag gccaccagct cgtcgaacac cctgtctgcg gcgacagcgg gcccgtggcc
     9001 cggggtgaac atcgacacgg tggtgaggcg atcggcgccg tggacgacca ccgcccgcac
     9061 cacgccgtcg gaggtcgaca cgcggaacgg gagggcgggc tgaccgccga ccgtggtcgc
     9121 cgtccccacg atcggggcgg caccgagacc ccggcccgcc tgcgccgcga cgtcggcgag
     9181 caccgaggtc acggggcggc cgcggagcgc cgactcgacc gacgtcaccg agatgagact
     9241 gccgtcggga ccgtcgaggt cgtactcggt ccgggtcacg gtgcccgatg cggtggcgac
     9301 cggcaccgac gccacccggg gcggggtcgg aaagctgacg gcgaagccgc ccacaaccgt
     9361 gcccggagcc ccggtggtcg tcggcgccac cggctcggtg gacggtggcg agctggactc
     9421 ggtggacgag gtcgtcgtcg acgaggtcga cgcggcggac gtcgaggtcg aggtcgaagc
     9481 ggcggtggac ggggtcggcg cggaggaggg tgacgccggg cctcgatccg ttccggcacc
     9541 ggcgcccgcg acggtcgacg gggatccgtc ggtcagggcc acgatccccg cgaggagcgc
     9601 gacgacgatg accgcggcga gcgcgatggt caacgaccag ctcggctcgc cggcggtgtg
     9661 gtcggttgcc ccagcgtcga ccatggtgac ggcgcctagc tgcccagcag atcggcgatg
     9721 cgctccttgg ggcgtccgac gacagcgcgg tcacccttca cgacgatcgg ccgctggagg
     9781 agcttcgggt tcgccgccag cagcgggatg acctgctcgg cggtctggac atcggcgtcg
     9841 gtcagaccga gctgggcgaa gttcgcgtcg cgtcgcacca gctcggtcac cgggccgtcc
     9901 agcttctcga cgatggatgc gatctcctcg gcgtcgggct tcgtcttgag gtagagcacg
     9961 acgtcgacgt cgacgccagc atctgccgcc atggcgagcg cggtcttcga ggagttgcag
    10021 ttcgggttgt ggtacacggt cacgtcggcc atgggcacag cgtgacagaa gctcgccggc
    10081 ccgaggtcac cgcatgacgt tcttttctca ggaatcaccg agcaaccggc cgatgagaca
    10141 aggcacgaac gcgatccgtc ctgaggaaac atgaatcaag aaccggcgat cagccagcac
    10201 gaactccgct cccccgcctc cgtcgacctc gcgggcatcc cgttgtcgat gatcgaggac
    10261 atcctcctgc ggacgacgat cgccgagggt cggccgtcga tggtgcgggt cgccgagcgg
    10321 ttgcactgcg gtgtcggcgt catcgagagc gtggtcagct cgctgcggga ccgtcgcctc
    10381 atggagtacg agggcatgca gggtcgcacc tacctgctct cggtcaccga ggcgggccga
    10441 gcccactccc gccagcgctc tgccgagtgc cggtatgccg gccccctgcc ggtgccgctc
    10501 gacctgtacc agcgggtggt ccgggcgcag cgcccgaacg tgcatctcga ccgcgaccag
    10561 ctgaccca
//
LOCUS       DAOSOA010000090        10551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6293_length_10551_cov_7.476658, whole genome shotgun sequence.
ACCESSION   DAOSOA010000090 DAOSOA010000000
VERSION     DAOSOA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_10551_cov_7.476658"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKY13_07620"
     CDS             complement(<1..389)
                     /locus_tag="PKY13_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79838.1"
                     /translation="MTQEILDSEGAYAAARLVPAAVRATLDVARRSGVPNDLDLGAVV
                     LAASARSKLTATTVAAIATTAVARPVITFVDEPLPVWVGPSTLVVALDAGVAERLVTD
                     TTVAGVPIIAVGGAVHDRPAPIVEAAAA"
     gene            complement(386..628)
                     /locus_tag="PKY13_07625"
     CDS             complement(386..628)
                     /locus_tag="PKY13_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOT79839.1"
                     /translation="MAISDVRLLEFLACPEDKGPLYYFEDEDTLYNPRLKRRYQIKDG
                     IPVMLIDEAESVSAAEDERLVAKAKADGIELNFVAS"
     gene            complement(674..2026)
                     /locus_tag="PKY13_07630"
     CDS             complement(674..2026)
                     /locus_tag="PKY13_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOT79840.1"
                     /translation="MTHNFNAIFKAYDVRGTVPDQFDDDMARAIGAAFAKFVSGEDPS
                     VGQVLVARDMRPSGEAMVAAFAEGVTSQGLDVVDLGLGSTDLLYFAAGTLDAPGAMFT
                     ASHNPAQYNGIKFCLSGARPVGEDSGLTQIRDDATAGVAPAATTGTVTRRDVLGAFAD
                     HVRSFVDVGALRPLKVVADTANGMGGLVVPAVFDGLPFDLEVMYGELDGTFPNHPADP
                     IQPENQRDLMARVLEVGADIGLAFDGDADRVFLVDEQGVGLSGSTTTAILADAILKSK
                     PGGKVVHNLICSKAVAEVVVEAGGEPIRSRVGHSFIKEIMASSGAVFGGEHSAHYYFA
                     DNFRADSGLIAAVLVLEQLSIRNLPLSELRKPYERYAASGEINTVVPDTGEVIERVAA
                     AYAGVQQDRLDGLTVDHGDWWFNLRPSNTEPLLRLNLEAADDEAVAARVAEVKAHFGL
                     "
     gene            complement(2064..3242)
                     /locus_tag="PKY13_07635"
     CDS             complement(2064..3242)
                     /locus_tag="PKY13_07635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT79841.1"
                     /translation="MRFARRFASVGAAVTLLDLGLVLLGARFTDWPLPVVDAVAIVVA
                     TFVSYWLHRAISYAAEPARRWYRDLRHYVMAALLALAADVAVFSILVQGSDHSVRSVV
                     IPKFLGLAAGFLVRLTSYRQTMFRSIRADQNRPRGRQAAPGDVRLSLVIPAYFEEDGI
                     AETVTAVEEALGHLRTDGGFELIVVDDGSTDATAERARAAGADRVIALEQNGGKGGAV
                     RAGVLAARGRTIAFTDADLSYSPDQVLRLLAEIEGGWDVVVGSRKHTETRTLVTARRL
                     REVGGRIVNAFTAFVLLGQYRDTQCGLKAFRSDVGKVIFSHSRIDGFAFDVEVFHLVE
                     RYRLTLAEVPVELTNSTRSTVHVVRDTTRLVRDLFRIRSVAHTGGYELEAEELATLGN
                     "
     gene            complement(3239..6265)
                     /locus_tag="PKY13_07640"
     CDS             complement(3239..6265)
                     /locus_tag="PKY13_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79842.1"
                     /translation="MDGHDPGSGLEDDAPVVAPVDQLDEFVDDQSDVQSGDDDYLAEL
                     EAERQQFLSTVAKVFSAVIVGLCCIYVFWVVHPELVFRNTTPTGGDMGAHVWGPAYLR
                     DELLPNFRVTGWTKDWYAGFPAYTFYMVIPSLMIVVLDVGVVTPNSFLGVFGALLIIG
                     VALVAAYRLRSVPSRLARFLGWTACLFVPLLTIDLPYNIAFKLIAVSGIVAFPAGVWY
                     LLKSLSLRRPGPELGAVASVAFLMDKTLFHIYGGNIASTMAGEFAFSISLTLSLFALG
                     SIAVGLRTGRHRARSAVLIALAMLCHVIPGVFFLLVGAVLMVALRPRVASLKWAVPVG
                     LSGGLMALWWYLPFYGRSAFLNDMGWEKLGVELPQQSWARLKVVGVLRGMGSTVADAT
                     SCDAKGTVVNWAQMWRNLLPLGPHEINGVKFDDPNMWAGKVFFVLAGVGVVLSIVMVV
                     RSGIWLTAVTLIAGVAFVVMPQDRFWNARVLPFYYLGIYLLAAVGVALLLRAMVLVVR
                     GRWADPPLAVSVAATTVVMLILWGALGMTLRTNPGGEMLASSPNGKPTYSWLYWTSTY
                     QGPVRDWAKWNFEGLEAKPGYSTVVEPVDPSNPNGPTRTVKTFNHASSDEYFGMINEM
                     ARVGRDEGCGRAFWEYDEKLNDYGTPMAPMLLPYFTDHCIGSMEGLYFEASSTTPFHF
                     LVQSELSQKPSRPERFDCHLGFDESPYRDFDIDAGIKHLQMLGVKYYMALTDASKAAA
                     NADSRLTKVGESGPWQVFEVADIDLVEPLANEPVVWDDVHDDIYTWARPAVDWFNDPD
                     EWGVLRASSGPSEWKRASDKDSTVPVTKAPGAKAKVTNVRTTQDTISFDVSEVGSPVL
                     IRSSYFPNWKAEGAGDVYRVTPNFMVVVPTDRHVTLTYTRAPMELISMFLSFLGLLGL
                     IYFVVRPPKVALAGREFFGDREPEDEPEPEPEFDPELDPELVVGAAEPAPEVDAGDIG
                     DGGDPPAGLVVDPGPGPAIEPDSGSRPDNSDETDPR"
     gene            complement(6331..6597)
                     /locus_tag="PKY13_07645"
     CDS             complement(6331..6597)
                     /locus_tag="PKY13_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79843.1"
                     /translation="MTKKSGRSNGTQDAARPATKTPEVRCGGCGETVVEINLLIDGEE
                     LTMRSCSNCDNRSWHRGGAPVELGGILADLSSSPTRYRRDLASR"
     gene            complement(6751..7470)
                     /locus_tag="PKY13_07650"
     CDS             complement(6751..7470)
                     /locus_tag="PKY13_07650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT79844.1"
                     /translation="MNVRKVLEPGPTVWWGWREAMAVVLGAHVVLIFAGGILVGSRGS
                     GGDPVPIAWQFGATVPFWVAAIGGSWWLGHRSDEGVRVSLGLFLRPFDLVIGAVTGVA
                     MQLVVIPVVYRLIVLPLSNSTMEDLERPAKELVNASTGAVATVLFVLMTCVCAPVAEE
                     VLYRAVLQRGAGRERAIVGIAAAAVVFGFAHFQPLQLIGLVLFGASSGFLVWRTGRLG
                     PGVVAHIAFNTATVVTLLANR"
     gene            complement(7477..7710)
                     /locus_tag="PKY13_07655"
     CDS             complement(7477..7710)
                     /locus_tag="PKY13_07655"
                     /inference="COORDINATES: protein motif:HMM:NF023430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HOT79845.1"
                     /translation="MSRICARPGCPRHAVATLSYAYSDGVVWVEDLAAEAHPMVHDLC
                     AHHADHLSVPRGWECRDVRAVATIGTGARRLSA"
     gene            7814..9823
                     /gene="ftsH"
                     /locus_tag="PKY13_07660"
     CDS             7814..9823
                     /gene="ftsH"
                     /locus_tag="PKY13_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT79846.1"
                     /translation="MAAEPTPPNNRTPNPQNPLSKRDQGLPSWSLWVILVIVLGAILA
                     SFNIDTTSGKKIQYSEFLTQVEAGEVKSATWDNSTTKITGEYKKGDESFETTGPPSPD
                     ESTMKLLRSKTELTFETTEPGLLGQLLPLLLPIALLIGFFFWMQRRAQGQMTGIMSIG
                     RSRAKTYSSERPATTFADVAGYEGVKQEIREVVDFLKEPERFGEIGARIPKGILLVGP
                     PGTGKTLIARAVAGEAGVPFLSVSGSDFMEMFVGVGASRVRDLFDSARKLGRAIIFID
                     EIDSIGRKRGAGLGGGHDEREQTLNQMLNEMDGFEATEGIVMMAATNRPDILDPALLR
                     PGRFDRQVVVPLPELSDRKQILDVHVRHKRLAPDIDLEIVARGTPGMSGAELSNLVNE
                     AALHAVRDGSQMIRNVDFEYARDRVLMGAKRESMVLSDKEKELTAFHEAGHAVCAAVL
                     EHTDPLHKVTIIPMGMALGVTISLPAEDRHSYDQAYLEDSIVMAMGGRIAERLIFGVV
                     STGANNDLVVATERARKMVREWGMSDRIGPMAWGSQGQVFLGEDLMSTRDYSDDTARV
                     IDEEVEKILRDCEQRCVDTLTRERAGLEMVARALLELETISGEDVTRYIAMSRGEAVP
                     PAPVAPPAPPAPASYPPPPAPAPSRIPSLGAIKPPFAPPPDFTGA"
     gene            complement(9872..10318)
                     /locus_tag="PKY13_07665"
     CDS             complement(9872..10318)
                     /locus_tag="PKY13_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT79847.1"
                     /translation="MERLLIVLPLVVVAVAVAFVVQRRRPDAPSQSVEHVAPAQLDRA
                     DFDRPEAPWLVVTFTSATCDTCAGVAAKAAILASDSVATVEVVYQTDRELHDRYHVTA
                     VPTLVIADADGVVQRSFIGATTATHLWAALAELREPGSVPPGCGGH"
     gene            complement(10321..>10551)
                     /locus_tag="PKY13_07670"
     CDS             complement(10321..>10551)
                     /locus_tag="PKY13_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79848.1"
                     /translation="GGTETIIADKIEIAPQQSLPIDVSAATSAGSIGVVVSSSTPVVV
                     SGRTTSTARAEISFVPAIPDAAKVSALPPLGG"
BASE COUNT         1826 a         3646 c         3603 g         1476 t
ORIGIN      
        1 gccgccgccg cctccacgat gggtgccggc cggtcgtgga ccgctccgcc gacggcgatg
       61 atcggtacac cggcgacggt cgtgtcggtc acgagccgtt cggccacacc ggcgtcgagc
      121 gccacgacga gggtggacgg accgacccac accggcagcg gttcgtcgac gaacgtgatg
      181 accgggcgcg ccaccgcggt ggtggcgatc gccgccaccg tcgtggcggt cagcttcgac
      241 cgggccgatg cggccagcac cacggcgccg aggtcgaggt cgttgggcac gccggagcga
      301 cgcgccacgt ccaaggtggc gcggaccgcg gcgggcacga gccgggctgc ggcgtaggcg
      361 ccctcgctgt cgaggatctc ctgcgtcaag aggccacgaa gttgagttcg atgccgtcgg
      421 ccttggcctt ggcgaccaga cgctcgtcct cggcggcaga caccgactcg gcttcgtcga
      481 tcaacatgac ggggatgccg tccttgatct ggtagcgccg cttgaggcgc gggttgtaga
      541 gggtgtcctc gtcctcgaag tagtagaggg gacccttgtc ctcggggcag gcgaggaact
      601 cgaggaggcg gacgtcggag atggccatgg caggagatcc taccggcgac caccccaccc
      661 ccggaaacct gtctcagagg ccgaagtggg ccttcacctc ggcgacccgg gcggcaaccg
      721 cctcgtcgtc ggccgcttcg aggttgaggc ggagcaacgg ctcggtgttg gaggggcgaa
      781 ggttgaacca ccagtcgccg tggtcgacgg tcagcccgtc gaggcggtcc tgctgcacac
      841 cggcgtaggc cgccgcgact cgttcgatca cctcgcccgt gtcgggcacc accgtgttga
      901 tctcgcccga cgcggcgtac cgctcgtagg gcttgcgcag ctcgctcagc ggcaggttgc
      961 ggatcgagag ttgctcgagc accagcaccg cggcgatgag gcccgagtcg gcgcggaagt
     1021 tgtcggcgaa gtagtagtgc gccgagtgct cgcccccgaa caccgcgccc gacgacgcca
     1081 tgatctcctt gatgaagctg tgaccgaccc gggagcgaat gggctcgcca ccggcctcga
     1141 ccacgacctc ggccacggcc ttggagcaga tgaggttgtg gacgaccttg cccccgggct
     1201 tcgacttcaa gatggcgtcg gcgaggatcg cggtcgtggt ggagccggac aggccgaccc
     1261 cctgctcgtc gacgaggaag acgcggtcgg cgtcgccgtc gaacgccagc ccgatgtcgg
     1321 caccgacctc gagcacccgg gccatgaggt cacgctggtt ctcgggctgg atcggatcgg
     1381 cgggatggtt cgggaacgtg ccgtcgagct cgccgtacat gacctcgagg tcgaacggga
     1441 ggccgtcgaa cacggcggga acgacgaggc cgcccatgcc gttggcggtg tcggccacga
     1501 ccttcagcgg ccggagcgcc ccgacatcga cgaacgaacg cacgtggtcg gcgaacgcgc
     1561 cgagcacgtc gcggcgggtc accgtgcccg tggtcgcagc gggggcgacg cccgccgtgg
     1621 cgtcgtcccg aatctgggtc agaccggagt cctcaccgac gggacgggcg cccgacaggc
     1681 agaacttgat gccgttgtac tgagccgggt tgtgcgacgc cgtgaacatg gcgccgggtg
     1741 cgtcgagcgt gccggccgcg aagtacagga ggtcggtcga accgagtccg aggtcgacga
     1801 cgtcgagccc ttgtgacgtg accccctcgg cgaaggccgc caccatcgcc tcgcccgatg
     1861 gccgcatgtc gcgggcgacc aggacctggc cgactgacgg atcctcaccg ctgacaaact
     1921 tggcgaacgc tgcgccgatg gcgcgggcca tgtcgtcgtc gaactggtcg ggcacagtgc
     1981 cccggacgtc gtaggccttg aagatggcgt tgaagttgtg cgtcatgacg gcgcacatcg
     2041 tacgccacga cctcgccgaa acctcagttg ccgagcgtgg ccagctcctc cgcctccagc
     2101 tcgtacccgc cggtgtgcgc caccgagcgg atccggaaca gatcgcgcac caaccgggtc
     2161 gtgtcccgca cgacgtgcac cgtcgagcga gtcgagttcg tcagctccac cggtacctcc
     2221 gcgagcgtca gccggtaccg ctcgacgagg tggaacacct cgacgtcgaa cgcgaacccg
     2281 tcgatgcgcg agtgggagaa gatcaccttg ccgacgtcgg accggaaggc cttcagccca
     2341 cactgggtgt cgcggtattg gccgaggagc acgaaggccg tgaacgcgtt gacgatgcgc
     2401 ccgccgacct cgcggagccg gcgagccgtc accagcgtgc gggtctcggt gtgcttccgg
     2461 gagccgacga cgacgtccca cccgccttcg atctcggcga ggagacggag cacctggtcg
     2521 ggcgagtagc tgaggtcggc gtcggtgaag gcgatcgtcc ggccccgcgc cgcgaggacg
     2581 ccggcgcgca ccgcacctcc cttgcccccg ttctgctcca gggcgatcac gcggtcggcg
     2641 ccggcggcac gcgctcgctc ggcggtcgca tcggtcgaac cgtcgtcgac gacgatgagt
     2701 tcgaacccgc cgtcggtccg cagatggccg agggcctcct ccacggcggt gacggtctcg
     2761 gcgatgccgt cctcctcgaa gtaggcgggg atgaccaggg agaggcggac gtcgccgggg
     2821 gcggcctgcc gaccgcgggg tcggttctgg tcggcgcgga tcgaccggaa catcgtctgc
     2881 cggtacgacg tcagccggac caggaacccg gcggcgagcc ccaggaactt cgggatgacc
     2941 accgagcgca ccgaatggtc cgacccctgg acgaggatgc tgaacacggc gacgtccgcg
     3001 gcgagcgcca gcaacgctgc catcacgtag tggcggaggt cgcggtacca ccggcgcgcc
     3061 ggctccgccg cgtaggagat ggcgcggtgc aaccagtacg agacgaacgt ggcgacgacg
     3121 atggcgacgg cgtcgacgac cggcagcggc cagtcggtga agcgcgcccc gagcagcacg
     3181 aggccgaggt cgagcagcgt gaccgccgca ccgaccgacg cgaagcgccg agcgaatctc
     3241 aacggggatc ggtctcgtcg ctgttgtcgg gacgcgatcc ggagtcgggt tcgatcgccg
     3301 gtccggggcc gggatcgacc acgagaccgg ctggcggatc accaccgtca ccgatgtcgc
     3361 cggcatcgac ctcaggcgcg ggctcggccg ccccgacgac gagctcggga tcgagctcgg
     3421 gatcgaactc tggctcgggc tcgggctcgt cctccggttc acggtcgccg aagaactccc
     3481 ggcccgcgag cgccaccttc ggcggacgca ccacgaagta gatgaggccg agcaggccga
     3541 ggaacgacag gaacatcgag atgagttcca tgggcgcccg cgtgtaggtg agcgtgacgt
     3601 gacggtcggt cgggaccacg accatgaagt tcggggtgac gcggtacacg tcgcccgcgc
     3661 cctcggcctt ccagttcggg aagtacgacg agcggatgag caccggtgag ccgacctcgc
     3721 tcacgtcgaa gctgatcgtg tcctgggtcg tccggacgtt ggtgaccttc gccttcgcac
     3781 cgggcgcctt cgtcacgggc accgtggagt ccttgtcgct cgcccgcttc cactccgacg
     3841 gaccgctcga cgcccgcagg acgccccact cgtcgggatc gttgaaccag tcgaccgcag
     3901 gtcgcgccca ggtgtagatg tcgtcgtgca cgtcgtccca cacgaccggc tcgttggcca
     3961 acggctcgac gagatcgatg tcggcgacct cgaagacctg ccagggaccc gactcgccga
     4021 ccttcgtgag gcgggagtcg gcgttcgcag cggccttcga cgcgtcggtc agcgccatgt
     4081 agtacttcac gcccaacatc tggaggtgct tgatgccggc atcgatgtcg aagtcgcggt
     4141 acggcgactc gtcgaacccg aggtggcagt cgaaccgctc gggccgcgac ggcttctgcg
     4201 acagctccga ctgcacgagg aagtggaacg gcgtggtcga cgacgcctcg aagtagaggc
     4261 cctccatcga tccgatgcag tggtcggtga agtacgggag gagcatcggg gccatcggcg
     4321 tgccgtagtc gttgagcttc tcgtcgtact cccagaacgc ccgaccgcag ccctcgtcgc
     4381 ggccgacacg ggccatctcg ttgatcatgc cgaagtactc gtcggagctc gcatggttga
     4441 acgtcttgac cgtgcgggtg ggaccgttgg ggttcgaggg gtcgaccggc tcgacgacgg
     4501 tgctgtaccc gggttttgcc tcgagcccct cgaagttcca cttggcccag tcccgcacgg
     4561 ggccctggta ggtgctcgtc cagtagagcc aggagtaggt cggcttgccg ttgggcgagg
     4621 acgccaacat ctcgccgccg gggttggtgc gcagcgtcat gccgagcgcg ccccagagga
     4681 tcaacatgac caccgttgtc gcggcgaccg acaccgcgag gggcgggtcg gcccagcggc
     4741 cgcgcaccac cagcaccatc gcccgcaaca gcagggcgac gccgacggcc gcgagcaggt
     4801 agatgccgag gtagtagaag gggagcaccc gggcgttcca gaagcggtcc tgcggcatga
     4861 ccacgaacgc caccccggcg atcagggtca ccgcggtgag ccagataccc gagcgaacca
     4921 ccatcacgat cgacagcacc acaccgacac cggccaacac gaagaagacc ttgccggccc
     4981 acatgttggg atcgtcgaac ttcaccccgt tgatctcgtg cggaccgagc ggcaggaggt
     5041 tgcgccacat ctgggcccag ttgacgacgg tgcccttggc atcacaactg gtcgcgtcgg
     5101 cgaccgtgct gcccatgcca cgcaacacac cgacgacctt cagacgcgcc cagctctgct
     5161 gcggcagctc gacgccgagc ttctcccatc ccatgtcgtt caggaacgcg ctgcggccgt
     5221 agaagggcag gtaccaccac agggccatga gcccgccgct gaggccgacc gggacggccc
     5281 acttgagcga ggccacccgc ggacgcaggg ccaccatgag caccgcgccg accagcagga
     5341 agaacacgcc ggggatcacg tgacagagca tcgccagggc gatcagcacc gccgaccggg
     5401 cccgatgccg gccggtgcgg agcccaacgg cgatcgaacc gagggcgaac agggacaggg
     5461 tcagcgagat cgagaaggcg aactcgcctg ccatcgtcga cgcgatgttg ccgccgtaga
     5521 tgtggaagag cgtcttgtcc atcaggaacg cgacggacgc gacggcgccc agctcggggc
     5581 cggggcgacg caacgacagc gacttcagca gataccagac accggcgggg aaggcgacga
     5641 tgccggagac ggcgatcagc ttgaacgcga tgttgtaggg cagatcgatc gtgagcagcg
     5701 gcacgaacag acacgccgtc caaccgagga agcgggcgag ccgcgagggc accgaacgga
     5761 gccggtaggc agccacgagc gcgaccccga tgatgaggag cgcaccgaag acgcccagga
     5821 acgagttcgg cgtgacgacg ccgacgtcga ggaccacgat catcaacgac gggatgacca
     5881 tgtagaaggt gtaggcgggg aaccccgcgt accagtcctt ggtccagccc gtgacccgga
     5941 agttcggcag caattcgtca cgcaagtacg cggggcccca cacgtgggcg cccatgtcac
     6001 caccggtggg cgtcgtgttg cggaacacga gctcggggtg cacgacccag aacacgtaga
     6061 tgcaacacag cccgacgatg accgcgctga agaccttcgc gacggtcgac aggaactgct
     6121 gccgctccgc ctcgagttcg gcgaggtagt cgtcgtcgcc ggactggacg tcggactggt
     6181 cgtcgacgaa ctcgtcgagc tggtcgacgg gagcgacgac cggtgcgtcg tcctccagtc
     6241 ctgaaccggg atcgtggccg tccatcagtg catcatcgtc tcatgtcagg acgccaggac
     6301 tcgggcacca ccgacccggc tcaccgggga tcagcgactg gccaggtctc gtcggtatcg
     6361 cgtcggggag ctactcaggt cggcgaggat accaccgagc tccaccggag caccgccgcg
     6421 gtgccagctg cgattgtcgc agttggagca cgaacgcatc gtcagctcct ccccatcgat
     6481 cagcaggttg atctcgacca cggtctcgcc gcatccgccg cagcgcacct ctggggtctt
     6541 cgtcgccggc cgggccgcat cctgcgtccc gttcgaacgg ccgctcttct tcgtcatcgg
     6601 tgattccttt cacctcgtgg gccccttctg atcgacgatc gggcacccgc gatgagggtg
     6661 gcggccgaga atggtccgga cgaactaagg cgatgccctc acaacctggg tggcggaccg
     6721 gatggcccat tcccaccccg gccgacggcc tcagcggttc gcgaggaggg tgacgaccgt
     6781 cgccgtgttg aaggcgatgt gcgccaccac accggggccg agacgcccgg tccgccacac
     6841 gaggaacccc gaggaggctc cgaacaggac gaggccgatc agttggagcg gctggaagtg
     6901 ggcgaacccg aagaccaccg ccgccgccgc aatgccgacg atcgcgcgct cgcgacccgc
     6961 cccccgttgc agcacggcgc ggtacaggac ctcttcggcc acgggagcgc acacacacgt
     7021 catgaggacg aacaacacgg tggcgaccgc gccggtgctg gcgttcacga gttccttcgc
     7081 cggacgctcc agatcctcca tcgtcgagtt cgacagcggg agcacgatga ggcggtacac
     7141 gaccgggatc acgacgagtt gcatcgccac accggtgacc gcaccgatga cgaggtcgaa
     7201 cggccggagg aacagtccga gcgagacgcg caccccctcg tcgctccggt gtccgagcca
     7261 ccacgagccc ccgatcgcag caacccagaa cggcaccgtc gcaccgaact gccacgcgat
     7321 ggggaccgga tccccacccg aaccccggga gccgaccagg atgccacccg cgaagatcag
     7381 gacgacgtgg gcgccgagca ccaccgccat tgcttcgcgc cagccccacc agaccgtcgg
     7441 gcccggttcg aggaccttcc ggacgttcac ccgcacctag gccgagaggc gccgcgcacc
     7501 ggtgccgatg gtggccacgg cgcgcacgtc acgacactcc catccccgcg gcacgctgag
     7561 gtggtcggcg tggtgggcgc acaggtcgtg caccatcggg tgcgcctcgg ccgcgaggtc
     7621 ctcgacccac acgacaccat cggagtaggc gtagctgaga gtcgctacag cgtgtcgcgg
     7681 acaaccgggc cgggcgcaga tcctgctcat cgccgctcga cggtagccgc cggacgggtg
     7741 attttcgcgc atggcggacc tgaggccgcg aacccgagta cggaaggtcc gacatccctt
     7801 ctaggctgtg cggatggctg ctgagcccac tcccccgaac aaccgcaccc cgaacccgca
     7861 gaacccactt tccaagcgcg accaaggcct tccgagctgg tcgctgtggg tcatcctcgt
     7921 catcgtcctc ggtgcgatcc tggcgtcgtt caacatcgac acgacgtcgg gcaagaagat
     7981 ccagtacagc gagttcctca cccaggtcga ggccggcgag gtgaagtcgg cgacctggga
     8041 caactcgacc accaagatca ccggtgagta caagaagggc gacgagtcgt tcgagaccac
     8101 gggcccgccc agccccgacg aatcgacgat gaagctgctg cgctcgaaga ccgagctcac
     8161 gttcgagacg accgagcccg gcctcctcgg ccagctcctg cccctcctcc tcccgatcgc
     8221 cctgctcatc ggcttcttct tctggatgca gcgccgggcg cagggccaga tgacgggcat
     8281 catgtcgatc ggtcgaagcc gagccaagac ctactcgagt gaacgccccg cgacgacctt
     8341 cgccgacgtc gccggctacg agggcgtgaa gcaggagatc cgcgaggtcg tcgacttcct
     8401 caaggaaccg gagcgcttcg gtgagatcgg cgcccgcatc cccaagggca tcctgctcgt
     8461 cggtcctccc ggcaccggca agacactcat cgcccgcgcc gtcgccggcg aggccggcgt
     8521 gccgttcctg tcggtctccg gttcggactt catggagatg ttcgtgggcg tcggcgccag
     8581 ccgcgtgcgt gacctcttcg actcggcccg caagctcggt cgggccatca tcttcatcga
     8641 cgagatcgac tcgatcggcc gcaagcgcgg cgccgggctc ggcggtggcc acgacgagcg
     8701 ggagcagacg ctcaaccaga tgctcaacga gatggacggc ttcgaggcca ccgagggcat
     8761 cgtgatgatg gccgccacga accgtcccga catcttggac ccggcgctcc tgcgcccggg
     8821 ccggttcgac cgccaggtcg tcgtgcccct gcccgagctg agcgaccgca agcagatcct
     8881 cgacgtgcac gtgcgccata agcgcctggc acccgacatc gacctcgaga tcgtcgcccg
     8941 cggcacgccc ggcatgagcg gcgccgagct ctcgaacctc gtcaacgagg cggccctgca
     9001 cgccgtacgc gacggctccc agatgatccg caacgtcgac ttcgagtacg cccgcgaccg
     9061 ggtcctcatg ggcgccaagc gcgagtccat ggtgctctcc gacaaggaga aggagctcac
     9121 ggcgttccac gaggcgggcc acgcggtctg cgccgcggtg ctcgagcaca ccgatcccct
     9181 gcacaaggtg accatcatcc cgatgggcat ggcactcggc gtgaccatct cgctgccggc
     9241 ggaggaccgc cacagctacg accaggcgta cctcgaggac tcgatcgtga tggccatggg
     9301 tggccgcatc gccgagcgcc tcatcttcgg ggtcgtgtcg accggggcca acaacgacct
     9361 cgtcgtcgcc accgaacgcg cccgcaagat ggtccgtgag tggggcatga gcgaccgcat
     9421 cggcccaatg gcgtgggggt cgcaggggca ggtgttcctc ggcgaggacc tcatgagcac
     9481 ccgcgactac tccgacgaca ccgcccgggt gatcgacgaa gaggtcgaga agatcctccg
     9541 cgactgcgag cagcgctgcg tcgacacgct cacccgcgaa cgcgccgggc tcgagatggt
     9601 ggcccgggcc ctgctcgagc tcgaaaccat cagcggcgaa gacgtgaccc gctacatcgc
     9661 gatgtcgcgt ggcgaagcgg tcccgccggc accggtggcg ccaccggcgc cgcccgcccc
     9721 ggcgtcgtac ccgccgccac cggctccagc gccgtcacgt atcccgtcgt tgggcgcgat
     9781 caagccgccg ttcgctcccc cgcccgactt cacgggagcc tgaccgcctc ctgatggcct
     9841 cggccgtcag tgtcgccgtc agtgtcggcc gtcagtggcc gccgcaaccc ggcgggaccg
     9901 agcccggctc gcgcagctcg gccagcgctg cccacagatg ggtcgccgtc gtcgccccga
     9961 tgaacgagcg ctgcaccacg ccgtcggcgt cggcgatcac cagggtcggg acagcagtga
    10021 cgtggtaccg gtcgtgcagc tcgcggtcgg tctggtagac gacctcgacg gtcgcgaccg
    10081 agtccgaggc caggatcgcc gccttggcgg cgacgccggc gcacgtgtca caggtcgccg
    10141 aggtgaaggt caccacgagc cagggcgcct cgggtcgatc gaagtcggca cgatcgagct
    10201 gcgccggggc aacgtgctcg actgattggg acggagcgtc gggccgacgc cgctgcacca
    10261 cgaaggcgac cgcaacggcg acgaccacca gcggaagcac gatcagcagg cgctccaccg
    10321 tcagcctccg aggggcggga gggccgacac cttcgccgcg tccgggatgg ccggcacgaa
    10381 cgagatctcg gcccgcgccg tcgaggtggt ccgaccggac accacaacag gtgtcgacga
    10441 cgacaccacc acaccgatcg atccggccga ggtcgcggcg ctcacgtcga tcgggaggga
    10501 ctgttgggga gcgatctcga tcttgtcggc aatgatggtc tccgtgccgc c
//
LOCUS       DAOSOA010000091        10529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6312_length_10529_cov_6.648845, whole genome shotgun sequence.
ACCESSION   DAOSOA010000091 DAOSOA010000000
VERSION     DAOSOA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6312_length_10529_cov_6.648845"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PKY13_07675"
     CDS             <1..890
                     /locus_tag="PKY13_07675"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOT79849.1"
                     /translation="VPAANTPDAEIEIDEPLIRHLLETQSPERARLSLGFLDQGWDNV
                     LFRLGDDEIVRLPRRQLGANLVEHEQTWLHRLAPGLPLQIPTPRFHGRPTERYPWRWS
                     ICPWIEGAPASVAPPTDTVAGATALGEFIAALHRAAPPDAPRNPHRGHPLAERDEITR
                     TRAAQLADLIDADVVVAAWARASVSTPWAGPPTWVHGDLHPGNLIVDDGRLVAVIDFG
                     DLCSGDPATDLGVAWMLFDTDGRAALRAAARVDDATWERGRAWAISLAVAVLSSSADR
                     PEYEAFAHHALNAAVVEHD"
     gene            985..2601
                     /locus_tag="PKY13_07680"
     CDS             985..2601
                     /locus_tag="PKY13_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT79850.1"
                     /translation="MTIQEKAYERRWWILVVLCLSLVVITLDNTILNVALPTLVEKLD
                     ASNSQLQWIVDGYTLVFAGLLLTAGSLGDRLGRKGALQIGLAIFGIGSALSAMATSPT
                     QLIITRCIMGIGGAFIMPATLSILTNVFPPEERGRAIGVWAGISGIGIAIGPLLGGYL
                     VDHFSWGSVFTVNLPVVAVAIIAGGLIVPSSKDPDAQKLDPVGAALSIVGLVSLVFGL
                     IEAPQEGWTDPKILAAFAIGALTLAAFAGWELRTDHPMLDVSFFKNPRFSAASTAITL
                     VFFGMFGFSFLLTQYLQFVLGYSPSQAGLRMMPLALTLMVVAPTSSRIVEKVGTKIVV
                     GTGLTLVSVALLLNTGLAVDSAYVQVVWRMMLLAVGMGLVMAPATESIMGSLPLGKAG
                     VGSAVNDTTRQVGGALGVAIVGSVLSSVYGTKIADFMGEAGLPAKAIDGARDGLGSAL
                     AIAGKVGGPEGAAIAGQARAAFVEALHGGAIVASVAAAVGAVVVIRYLPSHAPTIGDE
                     PQDSATDPTVSITVPSDATTTTESLDPASI"
     gene            2615..3244
                     /locus_tag="PKY13_07685"
     CDS             2615..3244
                     /locus_tag="PKY13_07685"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT79851.1"
                     /translation="MNADAITRPRGRPRDPGVDEGILGAALDLITEGGFEKLSMEAVA
                     ARAGVAKATIYRRFPSKVELIVAMCQAFGPAVQPPPNTGSFRTDVAAIIEALNDNMCP
                     TTDTGRLMPSMVSAAKENAEVREAMQRFTAARRRRIYDVVKAGIASGELRADADADAI
                     GDLLVGSMMYRLIIRGGKLDAKFRTALIDGLIDGFGASAPTSGGRPSPA"
     gene            complement(3213..4340)
                     /locus_tag="PKY13_07690"
     CDS             complement(3213..4340)
                     /locus_tag="PKY13_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79852.1"
                     /translation="MDEPTTDLPDATEPDDVVVPDPADLNGDGHVSQVEAAKKLLADA
                     DPAAYHKSISGLLRGGPGQLLQAMPYNPARPYHDPSCVVVEPNQSGTETMQLYCHAAY
                     RDFDQHLADPFDDPALDHLIASVYHGLVNEGINIALVTNHGQIHDIALVLAALVLALC
                     NDDRTFGVLGERTNLEEISLKCNLLLSRMVAATQVFSVPTTEVLQSMCRTFYSVPQTA
                     SRRRAKLDPELARANNLVMRDRLERQLADGGQLLAMAASGSQDISLASGLARKVRSAW
                     RQRRGEDRRDTPSLHLQPLYNGTINLMLACHYVLPVAISLDSEHPACEVGALTRVRTS
                     EDCHDVMHWIASAHEHATGIDTVYHQREDDLLTQVRDALRS"
     gene            4479..4715
                     /locus_tag="PKY13_07695"
     CDS             4479..4715
                     /locus_tag="PKY13_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79853.1"
                     /translation="MTAVARIGMQLNWAAATDIAAIGYYIVKRNGIVVILRKSAVNHL
                     DQQLTPATPYDDVIAVGTNDNPPPPLALTETTLL"
     gene            4712..5449
                     /locus_tag="PKY13_07700"
     CDS             4712..5449
                     /locus_tag="PKY13_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79854.1"
                     /translation="MSRPSRPLGAVVALAVAVLVAATACNDDDSSARSERTTTTAGAA
                     SGSSADGTATPASGSDGSTTTTLDTSTGTGTDTDGATADAWTAPGEPSPLELGTGVAE
                     PEYPAGNDGTVAEVANGELDLLVLQVDEAGGQVLVDVVELFVGDDAKAAATADGKLRP
                     DETLAGNYYVRNPNPKLRGVALDKDVVLRGEKGTGIATATGIARVSTALIDRLGAAPG
                     IPFLATVKAGKVTELVVPAVKDPTIPG"
     gene            5604..9554
                     /locus_tag="PKY13_07705"
     CDS             5604..9554
                     /locus_tag="PKY13_07705"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HOT79855.1"
                     /translation="MHRKLRASRPSRVLLAALAVTVAVPMLGTGNRTDRAADAAEPTV
                     KDGTTLAQAAVSCWAVKRTTPSATSGAYWLLTPQMTVPRRVWCDQETDGGGWELVGRG
                     RNGWTFTEGGQGTADSLADTPAGTGAFTPATLPTSFVTQLMGGLTVNQLAGGVRLRMA
                     ANTAGSSWQEVRWNFATATPWRWDFPTGLAVQSATLGTTTVTNRNTRDMTIGTKRIIT
                     SAVTANGSVQGFNNASSVHGSSAATSYLWEYNRVGYASPFTQVFIRPKLVEGTALAGE
                     TSVTFPQIPDAGLPAQQTTTSSAPKQTISSKPVAIGWGVATENDPNDQYTTLNTLVTS
                     IEIIGDTVFVGGKFNNVRNGANAPLIRQPYLAAFNRVTGDWISTFRPALDGAVWGLEA
                     TPDGKLLIAGNFSNIDGLPSTRALAKLDPATMAIDPTFAMDITRTGGLRPMGRALTIK
                     GDWAYLAGRFNGASGGTPTVANTGIGGLTKFRWATGEPDPNWRPYTDKTVEAISASPT
                     SDHLWMVGYFSTVSGTTAHSSASVSLTTGVRDDPPGMQTVIENSPIQMYQVNREPQWT
                     VGEWNGFVYQGGTQHYFGRHAISNYAQTGWSNQANGDYQTQEFLNGLVYAGCHCGATN
                     TTRQVNGQQVNTGLLSAFGAWDPDTFARSTTFQPDVRNWSEGPWAMETDPLTACLWVG
                     GDFLAGPADVFINGLAKFCVPGGVPDTTPPTTPTSVTAVAEAPGTRLRWSASTDDKAG
                     VLTYEVLLNDRLIGTTTATSFFSPFSGPGRYFVRAVDVAGNRSATSAVVSPADNQPVA
                     LVAFGSPWKYSADGSLPPAAWDTSGDVAAWPEAAAPLGKGGIGEVSTIPAAGTTQYFV
                     KDVQGTNLSTWGSVRVRLRADDGAIVRINGVQVARTNLGFGVSGPTAVAYQAVTAPGG
                     AATREITIPASLFVAGTNRISIELHQAIANDTDAFVDAELVVTRNGNDSSAPAAPTAP
                     TASSSTGTAIGVAWAVSPEIDVAGYELQRNGQVIGYFNRNEVAYFDQQLTSGTSYTYA
                     VVAYDQSGNRSAATSAVLSTTGTPPPPPPPVALSLVGPTSSWRFSADGTTPPAGWATT
                     DDLSAWGQGVGPLGAGGLGEATTIPTTAITQYLATDVTVTTPSTYRQIELTIAGDDAY
                     VVRINGTEVARSNIAFGVPTPTTPAYGEKVGPAPTATLRVPADLLVIGTNRISVELHQ
                     IRANDTDAYAALTATALPTNGDTVPPQMSTGSMTSIARTGIQLNWAAATDNAAIGYYA
                     VKRNGVVVTILRRTAVNYLDQQLTPATTYNYEVIAVDTNGNPSTPLALTATTLP"
     gene            9654..9890
                     /locus_tag="PKY13_07710"
     CDS             9654..9890
                     /locus_tag="PKY13_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79856.1"
                     /translation="MNERLGLRRLVGRRRGESYEVEEERYDDLRDTEGMVDLTEYAEL
                     ATRQREAYAYIDLSTAILEQDSVDVVDEGDWPAA"
     gene            complement(9944..>10529)
                     /locus_tag="PKY13_07715"
     CDS             complement(9944..>10529)
                     /locus_tag="PKY13_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT79857.1"
                     /translation="ADVDIIIAQGGEGGGHCGEVGSIVLWPQVVKAVAPRPVLAAGGI
                     GNGQQIAAALALGCQGAWSGSQWLMVEEAENTPVQQQAYIDASSRDTVRSRSFTGKPC
                     RMLRNDWTEAWENPENPDPLGMPLQYMVSGIAVAATHNYPDQTVDVAFNPVGQVVGQF
                     EKVEKTSAVIERWVNEYLEATSHLDELNARAAAG"
BASE COUNT         1753 a         3927 c         3333 g         1516 t
ORIGIN      
        1 gcgtgcccgc cgccaacacg cccgatgccg agatcgagat cgacgagccg ctgatccgcc
       61 acctgctcga gacccagtcg ccggaacggg cgcgcctctc actcggcttc ctcgaccagg
      121 ggtgggacaa cgtcctgttc cgactcggcg acgacgagat cgtgcggctg ccccgccgcc
      181 agctgggggc gaacctcgtc gagcacgagc agacctggct ccatcgcctc gctcccggtc
      241 tcccgctgca gatcccgact ccccggttcc acggacgtcc gacggagcgg tacccctggc
      301 gatggagcat ctgcccgtgg atcgagggtg ccccagcgtc ggtcgctccg ccgaccgata
      361 ccgtcgctgg tgcgaccgcg ctcggcgagt tcatcgccgc gctccaccgc gctgcaccgc
      421 ccgacgcgcc gaggaacccc catcgtggcc acccgctcgc cgagcgcgac gagatcaccc
      481 gcacccgagc cgcgcagctc gccgacctga tcgacgccga cgtggtcgtc gccgcctggg
      541 cccgcgccag cgtttccaca ccgtgggccg gtcctcccac ctgggtgcac ggcgatctcc
      601 atcccggcaa cctcatcgtc gatgacggcc gactcgtcgc cgtgatcgat ttcggtgacc
      661 tgtgctcggg tgacccggcg accgacctcg gtgtggcgtg gatgctgttc gacaccgacg
      721 gtcgtgccgc gctgcgcgcc gctgcacgcg tcgacgacgc gacgtgggaa cgcggtcggg
      781 cttgggcgat cagcctggca gtggcggtgt tgtcgtcgtc ggccgatcgc cccgagtacg
      841 aggccttcgc ccatcacgcc ctgaacgcgg ccgtcgtcga gcacgactga gacttcggtc
      901 actttcgata cgagaccgtt gcggatcgta aatggtcgcg cctacattgc gagacgcact
      961 cgtatcgaaa ccagaggtgg ggcaatgacg atccaagaga aggcgtacga acggaggtgg
     1021 tggatcctcg tggtcctctg tctctcgctc gtggtgatca cgctcgacaa caccatcctg
     1081 aacgtcgcgc tcccgaccct ggtcgagaag ctcgacgcct ccaacagcca gctgcagtgg
     1141 atcgtcgacg gctacaccct cgtcttcgcc ggcctcctcc tgaccgccgg cagtctggga
     1201 gaccgcctcg gtcgcaaggg tgcactccag atcggcctcg ccatcttcgg catcggctcc
     1261 gcactctcgg ccatggcgac cagcccgacc cagctcatca tcacccgctg catcatgggc
     1321 atcggtggcg cgttcatcat gcccgccaca ctctcgatcc tgaccaacgt cttcccgccc
     1381 gaggagcgtg gtcgcgccat cggagtgtgg gccggcatct ccggcatcgg catcgccatc
     1441 ggcccgctgc tcggcggcta cctcgtcgac cacttctcgt ggggctcggt cttcaccgtc
     1501 aacctgccgg tcgtcgccgt ggcgatcatc gctggcggcc tgatcgtccc gagctcgaag
     1561 gaccccgacg cgcagaagct cgaccccgtc ggcgccgccc tgtcgatcgt cggcctcgtg
     1621 tcgctcgtct tcggcctcat cgaagcgccg caggaaggct ggaccgatcc gaagatcctc
     1681 gcagcgttcg cgatcggcgc actcacgctc gccgccttcg ccggatggga actgcgaacc
     1741 gaccacccga tgctcgacgt cagcttcttc aagaaccccc gcttcagcgc ggcgagcacc
     1801 gcgatcacgc tggtgttctt cgggatgttc ggcttctcgt tcctgctcac gcagtacctg
     1861 cagttcgtac tcggctactc cccctcccag gccggcctgc gcatgatgcc gctggcgctg
     1921 accctgatgg tcgtcgcacc cacctcctcg cggatcgtcg agaaggtcgg caccaagatc
     1981 gtcgtcggca ccggcctcac cctcgtgtcc gtcgcgctcc tcctcaacac gggcctcgcc
     2041 gtcgacagcg cctacgtcca ggtcgtgtgg cgcatgatgc tcctcgccgt cggcatgggc
     2101 ctcgtcatgg caccggcgac cgagtcgatc atgggatcgc tcccactcgg caaggccgga
     2161 gtcggatccg ccgtcaacga caccacccgc caggtcggcg gcgcactcgg cgtcgccatc
     2221 gtgggcagcg tcctctcctc ggtctacggc acgaagatcg ccgacttcat gggtgaggcc
     2281 ggcctgccgg ccaaggcgat cgacggcgca cgggacggcc tgggcagcgc cctcgccatc
     2341 gccggcaagg tcggtggtcc cgaaggcgcg gcgatcgccg ggcaggcccg cgccgcgttc
     2401 gtcgaagcgc tccacggcgg agcgatcgtc gcctccgtgg cagcggcggt cggcgccgtc
     2461 gtggtcatcc gctacctccc gtcccacgcc ccgaccatcg gcgacgagcc ccaggattcg
     2521 gcgaccgacc ccaccgtgtc catcacggtt ccctccgacg cgactacgac gacggagtcg
     2581 ctcgaccccg cgtcgatctg atgagctggt cgacatgaac gccgacgcca tcacgagacc
     2641 ccgaggacgc ccccgcgacc ccggggtcga cgaaggcatc ctcggcgccg cgctcgacct
     2701 catcaccgag ggcggattcg agaagctgag catggaagcg gttgcggcac gggcgggcgt
     2761 cgcgaaggcg acgatctacc gacgattccc gtcgaaggtg gaactcatcg tcgccatgtg
     2821 ccaggcgttc gggccggccg tccaacctcc gcccaacacc gggtcgttcc gcaccgacgt
     2881 cgcggccatc atcgaagcgc tcaacgacaa catgtgcccc accaccgaca ccgggcgcct
     2941 catgccgtcg atggtgagcg ccgccaagga gaacgccgag gttcgagagg ccatgcagcg
     3001 gttcaccgcc gcacgccgcc gacgcatcta cgacgtggtg aaggccggca tcgcgagcgg
     3061 cgagctccgc gccgacgccg acgccgacgc gatcggcgac ctgctcgtcg gctcgatgat
     3121 gtaccgcctc atcatccgcg gcgggaagct cgacgcgaag ttccgcaccg ccctcatcga
     3181 cggcctcatc gacggcttcg gtgcgtcggc ccctacgagc ggagggcgtc cctcacctgc
     3241 gtgaggaggt cgtcctcccg ctggtgatag acggtgtcga tgcccgtcgc gtgctcgtgc
     3301 gccgaggcga tccagtgcat cacgtcgtgg cagtcctccg acgtccgcac ccgcgtgagc
     3361 gccccgacct cgcacgccgg gtgctcggaa tcgagcgaga tcgccacggg caacacgtag
     3421 tggcacgcca acatcaggtt gatcgtgccg ttgtacagcg gttggaggtg gaggctcggc
     3481 gtgtcgcgac gatcctcacc ccggcgctgg cgccacgctg accgaacctt gcgggcgagg
     3541 cccgacgcga gcgagatgtc ctggctgccg ctggcagcca tcgccagcag ctgtccaccg
     3601 tcggcgagct gccgctcgag ccgatcacgc atcacgaggt tgttggcccg cgccagctcg
     3661 ggatcgagct tggcccgacg gcgactcgcc gtctggggaa cgctgtagaa cgtgcggcac
     3721 atcgactgca gcacctcggt cgtgggcacg ctgaagacct gcgtcgccgc caccatacgg
     3781 ctgagcaaca ggttgcactt gagcgagatc tcctcgaggt tcgtccgctc cccgagcaca
     3841 ccgaacgtgc ggtcgtcgtt gcacaacgcc aacacgagcg ccgccagcac cagcgcgatg
     3901 tcatggatct ggccgtgatt ggtgaccagg gcgatgttga tgccttcgtt gaccaggccg
     3961 tggtacacgc tggcaatgag gtgatcgagc gccggatcgt cgaacggatc ggcgaggtgt
     4021 tgatcgaagt cgcggtacgc ggcgtggcag tagagctgca tcgtctcggt gccggactgg
     4081 ttcggctcga cgaccacgca cgacggatcg tggtacggcc gagcagggtt gtacggcatc
     4141 gcctgcaaca gctggccggg gccaccacgg agcagcccag agatgctctt gtgatacgcc
     4201 gccggatcgg cgtcggccag cagcttcttg gccgcctcga cctgggagac gtggccatcg
     4261 ccgttgaggt cggccgggtc gggcaccacg acgtcgtcgg gctccgtcgc gtcgggcagg
     4321 tcggtggtcg gctcatccat cgccgtcaag cgtagggcgt cgcccgaggg gcaacgttcg
     4381 agaccacccg cgtgggccgc gcactgtcaa aggcgccccc gggaaccgca ctcccgatca
     4441 acggcgatac cgttccatgc cagaggtcca ccagctcgat gaccgctgtc gcccgcatcg
     4501 gcatgcagct caactgggcg gccgctactg acatcgccgc catcggctac tacatcgtca
     4561 agcgcaacgg catcgtcgtc atcctccgaa agagcgcagt gaaccacctc gaccaacagc
     4621 tcacccccgc taccccctac gacgacgtca tcgccgtcgg caccaacgac aacccgcccc
     4681 caccactcgc actcaccgaa acgaccctgc tgtgagccgg ccctcccgtc ccctcggcgc
     4741 cgtcgtcgcc ctcgccgtcg ccgtgctggt cgcagcgacg gcgtgcaacg acgacgactc
     4801 gtcggcccgc tccgaacgca ccacaacgac ggcaggggcc gcgtcggggt cgagtgccga
     4861 cggcacggcg acgcccgcct ccggctccga cggctcgacc accaccacac tggacaccag
     4921 caccggcacc gggaccgaca ccgacggagc gacggccgac gcgtggaccg ctccgggaga
     4981 accgtcgccg ctcgagctgg gaacgggcgt cgccgagccc gagtaccccg ccggcaacga
     5041 cggcaccgtg gccgaggtcg ccaacggcga actcgacctc ctggtcctgc aggtcgacga
     5101 ggcgggaggc caggtgctcg tcgacgtcgt cgagctgttc gtcggtgacg acgccaaggc
     5161 cgcagcgact gccgatggca agctgcggcc cgacgaaaca ctcgccggca actactacgt
     5221 ccgcaatccc aatccgaagc tcagaggggt ggccctcgac aaggacgtgg tgctccgcgg
     5281 cgagaagggc accggcatcg ccacggcgac cggcatcgcc agggtgagta cggcgctcat
     5341 cgaccggctc ggcgctgcac cgggcatccc cttcctggcg acggtgaagg cgggcaaggt
     5401 caccgagctc gtggtaccgg ccgtgaagga tccgaccatt ccggggtgag cgctcccgtg
     5461 gtcccacgga tggcggtcgg cgacacgtag gccgatcggc ccttgtccta cccaggcaac
     5521 tctcgcgatc atggggtccc gaacgtcggc ggaagacgct cggaacgtgc gaggcggacc
     5581 agacaaccat gacaatcact cggatgcacc gaaagctccg ggcttcgcga ccctcccggg
     5641 tcctgctcgc ggcactggcc gtcaccgtgg ccgtcccgat gctcggaacc ggcaaccgga
     5701 ccgaccgggc agcagacgcc gccgagccca ccgtcaagga cggcaccacg ctcgcccagg
     5761 cagcggtgtc gtgttgggcc gtcaaacgca cgacgccctc ggcaacctcg ggtgcctact
     5821 ggctcctcac cccccagatg acggtgccgc ggcgcgtgtg gtgcgaccag gagaccgacg
     5881 gcggcggctg ggaactcgtc ggccgtggcc gtaacggttg gaccttcacc gaaggcgggc
     5941 agggcaccgc cgacagtctc gccgacaccc cggcgggcac cggcgcgttc acccccgcca
     6001 cccttcccac ctcgttcgtc acgcagctca tgggtggcct caccgtcaat cagctggccg
     6061 ggggcgtgcg gctccgcatg gccgccaaca ccgccggttc gagctggcag gaggtccgct
     6121 ggaacttcgc gacggccacg ccgtggcggt gggacttccc gaccgggctc gcggtccaga
     6181 gcgccacact cggaacgacg acggtcacca accgcaacac ccgggacatg acgatcggca
     6241 ccaagcgcat catcacctcg gcggtcacgg ccaacggcag cgtccaaggc ttcaacaacg
     6301 cgtcgagcgt gcacggttcg agtgctgcga ccagctacct gtgggagtac aaccgcgtcg
     6361 gctacgccag cccgttcacc caggtcttca tccgcccgaa gctggtcgag ggcaccgcgc
     6421 tcgccggcga gacgtcggtg acgttcccgc agattcccga cgccggactc cccgcacaac
     6481 agacgacgac ctcgagcgct cccaagcaga cgatcagctc gaagcccgtg gccatcggct
     6541 ggggcgtcgc caccgagaac gaccccaacg accaatacac caccctcaac acgctggtga
     6601 cgtcgatcga gatcatcggc gacacggtct tcgtcggagg caagttcaac aacgtccgca
     6661 acggcgcgaa cgctccgctc atccgccagc cctacctggc ggcgttcaac cgcgtcaccg
     6721 gcgactggat ctcgacgttc cggcccgccc tcgacggcgc ggtctggggg ctcgaagcga
     6781 cgcccgacgg caagctgttg atcgccggca acttcagcaa catcgatggt ctgcccagca
     6841 cccgggcatt ggccaagctc gaccccgcga ccatggcgat cgacccgacc ttcgccatgg
     6901 acatcacccg caccggcgga ctccggccga tgggacgcgc cctcacgatc aagggcgact
     6961 gggcgtacct cgccggccgc ttcaacggtg cgagcggcgg caccccgacc gtcgccaaca
     7021 ccggcatcgg tgggctcacc aagttccgat gggcaaccgg cgagcccgac ccgaactggc
     7081 ggccgtacac cgacaagacc gtcgaggcga tctcggcgtc gccgacgagc gaccacctct
     7141 ggatggtggg ctacttcagc acggtcagcg gcaccacggc gcattcgtcg gcatcggtct
     7201 cgctcacgac cggggtgcgc gacgaccctc cgggcatgca gacggtcatc gagaactcgc
     7261 cgatccagat gtaccaggtc aaccgcgagc cccagtggac cgtcggcgag tggaacgggt
     7321 tcgtctacca gggcggcacc cagcactact tcggccgcca cgccatctcc aactacgccc
     7381 agaccggctg gtccaaccaa gccaatggtg actaccagac ccaggagttc ctcaacggcc
     7441 tggtctatgc gggctgccat tgtggcgcca ccaacacgac ccggcaggtc aacggccagc
     7501 aggtcaacac cggcctgctc tcggcgttcg gcgcctggga ccccgacacg ttcgcccgga
     7561 gcaccacctt ccagcccgac gtgcggaact ggagcgaggg accgtgggca atggagaccg
     7621 accccctcac cgcgtgcctg tgggtgggag gcgacttcct cgcaggaccg gccgacgtgt
     7681 tcatcaacgg cctcgcgaag ttctgcgtgc ccggcggtgt tcccgacacc acgccgccca
     7741 ccacgcccac cagcgtcacg gccgtggccg aggcgcccgg cacgcggctc cggtggtcgg
     7801 cctccaccga cgacaaggcc ggtgttctca cctacgaagt gctcctcaac gaccgactga
     7861 tcggcaccac caccgccacg agcttcttca gcccgttctc gggacccggt cgatacttcg
     7921 tgcgcgccgt cgacgtcgcc ggcaaccgct cggcgacgag cgcagtcgtc agtccggccg
     7981 acaaccagcc ggtcgccctc gtggccttcg ggtcgccgtg gaagtactcc gccgacggat
     8041 cgttgccgcc ggcggcgtgg gacacgagcg gcgatgtggc ggcatggcct gaagcggccg
     8101 cgccactcgg caagggaggc atcggcgagg tctcgacgat ccccgctgcc ggcaccaccc
     8161 agtacttcgt gaaggacgtg cagggcacca acctctccac gtggggcagt gttcgggtgc
     8221 gactccgcgc cgacgacggc gccatcgtcc gaatcaacgg cgtccaggtc gcccggacca
     8281 acctcgggtt cggcgtgtcg ggcccgaccg ccgtcgccta ccaggccgtg accgcgcccg
     8341 gcggagccgc gacccgcgaa atcaccatcc cggcgtcgct cttcgtcgcc ggcaccaacc
     8401 gcatctcgat cgagttgcac caggccatcg ccaacgacac cgatgcgttc gtcgacgccg
     8461 agctggtcgt cacccgcaac ggcaacgact cgtcagcacc ggcggcaccg acggcaccga
     8521 cggcctcgag cagcacgggc accgccatcg gcgtcgcgtg ggccgtgtcg cccgagatcg
     8581 acgttgccgg ctatgagctg cagcgcaacg gccaggtgat cggctacttc aaccgcaacg
     8641 aggttgcgta cttcgaccag cagctgacct cgggcacctc ctacacctac gccgtcgtgg
     8701 cctacgacca gtccggcaac cgctcggccg ccaccagcgc ggtcctgtcc acgacgggca
     8761 ccccgccgcc accacccccg ccggtcgcgc tctccctggt cggtccgacc tcgagctggc
     8821 ggttctcggc cgacggaacg actcccccgg caggatgggc caccaccgac gacctgtcgg
     8881 cgtggggtca aggcgtcggc cccctcggcg ccggcgggct cggcgaggcc accaccatcc
     8941 cgaccacggc gatcacccag tacctcgcca ccgacgtcac cgtcaccaca cccagcacct
     9001 accgccagat cgagctgacc atcgccggcg acgacgccta cgtcgtccgc atcaacggca
     9061 ccgaggtggc ccgctccaac atcgcgttcg gtgtgccgac acccacgacg cccgcatatg
     9121 gcgagaaggt ggggccggcc ccgaccgcca cgctccgcgt ccccgccgac ctcctggtga
     9181 tcggcacgaa ccggatcagc gtcgaactcc accagatccg agccaacgac accgacgcct
     9241 acgccgcact gacggcgacc gcgttgccga ccaacggcga caccgtgccc cctcagatgt
     9301 ccaccggctc gatgacctcc atcgcccgaa ccggcatcca gctcaactgg gcagccgcca
     9361 ccgacaacgc cgcgatcggc tactacgccg tcaagcgcaa cggtgttgtc gtgacgatcc
     9421 tccggaggac cgccgtgaac tacctcgacc agcagctcac ccccgccacc acctacaact
     9481 acgaagtgat cgccgtcgac accaacggca acccgtccac gccactcgca ctcaccgcaa
     9541 cgacccttcc gtgaccgatc cggagtgaac ccccggactc gtcgaaacgg ggggatgccg
     9601 cgaccacggc gcaccactac ggtgacgcca atcggagagt gagggagctg gggatgaacg
     9661 agcggctggg gctgcgacga ctcgtcggac gacgacgcgg cgagagctac gaggtcgagg
     9721 aggagcgcta cgacgacctt cgcgacacgg agggcatggt cgacctcacc gagtacgccg
     9781 agctggcgac ccggcagcgg gaggcctacg cctacatcga tctgtccacc gccatcctcg
     9841 agcaagactc cgtcgacgtc gtggacgagg gcgactggcc cgcagcatga acgaaccccg
     9901 gccgtgacgg ccggggttcg aacagcaact ccgatgggat cggtcagccg gcggcagcgc
     9961 gggcgttgag ctcgtcgagg tggctggtgg cctcgaggta ctcgttgacc caccgctcga
    10021 tgacggccga ggtcttctcg accttctcga actggccgac gacctggccg acagggttga
    10081 acgcgacgtc gacggtctgg tcggggtagt tgtgcgtcgc cgcgacggcg atgccggaga
    10141 ccatgtactg cagcggcatg ccgagcgggt cggggttctc ggggttctcc cacgcctcgg
    10201 tccagtcgtt gcggagcatg cggcacggct tgccggtgaa cgaccgcgag cgcaccgtgt
    10261 cacgcgacga agcgtcgatg taggcctgct gctggacggg cgtgttctcg gcctcctcga
    10321 ccatcagcca ctgcgagccc gaccacgcgc cctggcaacc gagcgccaga gctgccgcga
    10381 tctgctgacc gttgccgatg ccaccggcgg cgagcacggg ccgcggggcc acggccttga
    10441 cgacctgggg ccacaacacg atcgacccga cctcaccgca gtggccgcca ccctcgccgc
    10501 cctgggcgat gatgatgtcg acgtcggcg
//
LOCUS       DAOSOA010000092        10377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6434_length_10377_cov_5.482174, whole genome shotgun sequence.
ACCESSION   DAOSOA010000092 DAOSOA010000000
VERSION     DAOSOA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_10377_cov_5.482174"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKY13_07720"
     CDS             complement(<1..208)
                     /locus_tag="PKY13_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79858.1"
                     /translation="MPDTPGHAEVPQRKQLPRDEGASLSEGGRGHRLVLIDHPAPHVA
                     RITLNRPERLNAMSIDLVIELWDAL"
     gene            complement(219..1841)
                     /locus_tag="PKY13_07725"
     CDS             complement(219..1841)
                     /locus_tag="PKY13_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOT79859.1"
                     /translation="MLPRTHLRRAGAALVLIGLVGSVACTSGKSSSSTTTTTAPAACA
                     PKTGEKAAKALLRCAEGSVAYVENEDASGTGVVIERDGDRYVLTNAHVVDPFATADVT
                     VGGERYDALPILGVDAGADIALVGPFDGKKGPSPLPVDPEAAVERGDDVYLVGFPGEV
                     STDDPEDLEATIASGIVSRTRKVKAFEQTYFQTDASIAGGQSGGPLFDAAGHLIGISG
                     LSFADDEFALVLSGGDVDEAIARILAGKGDDEYPAVPRVETDDMVKSGTLTVSGASDY
                     QQLFIPSSDDDRTLDFTVDHTDVVTVDISTNLSDSSLAVSHNTPLLMAELMKQVAAAT
                     GGKPDELPDPTAYGVDEKAMKPETSPGVFSVEIPADEDVYIGIVAPLSTDSITVAFTS
                     SIPAVPLTTKLSEQPIEVGDVVDRVIPTYESAADYLLDLEAGQKVEIRAESPQSDMAF
                     AVIDPGVVLTPVANFSPESAEGIELVDDSDEGLYGLDAVTTVTADDAGTYRIRLYSNE
                     GLTALARLSVTDCENAKCGKGDKKASEDEGSN"
     gene            complement(1902..3200)
                     /locus_tag="PKY13_07730"
     CDS             complement(1902..3200)
                     /locus_tag="PKY13_07730"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT79860.1"
                     /translation="MTGSTTSDRPLLLPSAGAVDVAALDAGVRVSVLSSGVRVATDPM
                     AHSQSVAIACFVGVGSRDEPSELAGASHFLEHLLFKGTMRRTAREINRAVDAVGGDFN
                     AYTVRESTVFYVRVPAAHAQFATDLLCEVIASPRFDPADVEIERNVILGELDGALDSP
                     DDVVFMNLAEAMFVGHSLGRETLGDPDTLERMTADEIAGFHHRWYRPSNLVFSVAGAV
                     DHDLVAAAIDARFDGAVDGERPDRVPPGSSMTGLVEARRPIEQVHLALGWRGVTALDG
                     DRVPLGVMNHALGDGPSSRLHEEIRERRGLAYSVSSMAASNLDAGTQTVYCATGPEHL
                     SEVATIIDETIAAIVADGIDDEELAVAKGYLSGSMLMALEDSSSRMSRLGSAVLTHGR
                     HIPIRESLDAVEAVTHDDVRRVAGQVFSGSRVTSLVGPDT"
     gene            complement(3233..5746)
                     /locus_tag="PKY13_07735"
     CDS             complement(3233..5746)
                     /locus_tag="PKY13_07735"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOT79861.1"
                     /translation="MADAIRVESTVGGDETKTMSFETGKLASLAGGAVLAAIGDTQIL
                     VTATAAKGVRDGIDFFPLTVDIEERSYAAGKIPGSFFRREGKSSDQAILTCRLIDRPL
                     RPSFPEGFRNEVHIVGTVIAADMENPHDVLAINAASAALMISDVPFDGPIGAVRIAYD
                     VGGEWVAHPTYQEGETSVFEMVVAGRELADGDVAVMMVEAGGTENSWEFYSDGAPKVD
                     EAALAAGLQASKQWIKEAIALQNQLIEASGGKKSVMDFPVQVDYTDEQYSAVSDAAGA
                     KISEIMTIADKHDRTAAEGALRDETVGALIDQFAGGDAALALKQLKAAFKSLTKATVR
                     SRIVNDGARIDGRGTRDLRPLSAEVGYVPTAHGSGLFQRGETQVLNVATLAMGKMDQM
                     IDGITPLTSKRYMHHYNFPPFSTGETGFMRGPKRREIGHGLLAERALLPVVPPMAEFP
                     YVIRCVSDVLASNGSTSMASVCGSTLSMMDAGVPLRAPVAGIAMGLVFAEGKYTTLTD
                     ILGAEDAYGDMDFKVAGTAEFVTALQLDTKIDGIPADVLSAALEQAKEARLAILEVMA
                     TAIPENRSEVSPNAPKIVSFEIPADKIGEIIGPKGKVINAIQAETGANISVDDDGMVG
                     IVSIAAVDPACVTEAERQIRLILNPPTAGVGEVYQGKVVNITKFGAFVNILPGRDGLV
                     HISKLGGGKRIDSVESVLSLGDEIEVRVEDIDPNGKISLRPTASDSADGGSDPKAEAA
                     GAASEGVSAETASRHQDDAGSAAASGGRPRGAASASVSDEPAPPESGDAVLVSFEDFQ
                     DEKTREAQGELGPVAKGVGAGAGGGGGRDRNRARRPRRR"
     gene            complement(6061..6339)
                     /gene="rpsO"
                     /locus_tag="PKY13_07740"
     CDS             complement(6061..6339)
                     /gene="rpsO"
                     /locus_tag="PKY13_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOT79862.1"
                     /translation="MSRPPANLPAKADTIAKHARGKGDTGSPEVQIALLTDRINHLTA
                     HLKDHKKDHHSRRGLLMLVGRRRRFLDYLKEIDIERYRAIVAELGLRR"
     gene            complement(6559..7641)
                     /locus_tag="PKY13_07745"
     CDS             complement(6559..7641)
                     /locus_tag="PKY13_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT79863.1"
                     /translation="MSHVVEPIPGPVPEGVPGDGVPVAAPRLARHRITISDGHTVGIT
                     VAGAGIPLVVVHGFSVEGFLYAQTLSRLVSMGFKVIAVDTAGHGGTRGLPADGADLKA
                     YSELLGRIIDDLGIEQCILAGHSMGGRLVTQLAAVRPERTIAVMLIDAIVGDTWDKMV
                     YAFRLAPPLVGVVGAALTVDAITILPVWRDPRQAIKFLRLALPTIVGDIVHPWRLLGP
                     VVSILRSRSSRYALNELAHEDVPVYVMHGDRDLAVPFRTAREAAERTEGTLITIKGGG
                     HSWLLRDPETLPGIVAQLLPGTLGEACRAAIRRGGVTAKEPTLSDIERALYRPDARIF
                     ELTPASNVTTVAGRHRRPKFRWTIAD"
     gene            complement(7638..8594)
                     /locus_tag="PKY13_07750"
     CDS             complement(7638..8594)
                     /locus_tag="PKY13_07750"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOT79864.1"
                     /translation="MRIARDLTELAAPGPGSVVSIGFYDGVHLGHQALISSVLDRAAA
                     AGLTTTVVTFDRHPAQVLRPESAPLLLCDLDQKLERLGETGIDQTLVVHFDEERARET
                     AEHFVRDVLVGGLGAKVVVVGEDFHFGHRRSGNVAMLQEMGAELGFEVIGHHLVGLDW
                     TDARPELQVSSTAIRRALVEGRLDDANAMLGRPHEMRGPVVDGDKRGRTIGFPTANVA
                     IRSEILLPSDGIYAGHLERPTGERLPSAIYLGTRPTFYDDQPASLLEVHCLDWEGDLY
                     GEDVRVHFEHRLRGDRKFDTIEELTAQLAIDCQASSELLRPR"
     gene            complement(8606..9493)
                     /gene="truB"
                     /locus_tag="PKY13_07755"
     CDS             complement(8606..9493)
                     /gene="truB"
                     /locus_tag="PKY13_07755"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT79865.1"
                     /translation="MGRRGKDSGDGPIHGVAIVDKDAGWTSHDVVAKARGILGTRKVG
                     HSGTLDPDATGVLVLGVGRATKLLRFLQLLPKSYECEIVFGTETNTLDAAGEVTATYD
                     MTVDPAEATAATAQFTGDIQQVPPMVSAVKIDGKRLHELARKGIEVDRPPRPVTVYRY
                     DVEPTDDPLVYRAFVDCSSGTYVRVLAADLGHALGGGAHLRKLRRTSVGAFTLADATS
                     LEDVTVIPMAEAVRGFPIVRVDEATEAAIRVGAVLDSAALGVAGTGPWPILSPTGELL
                     AMYEPFGDGRVKPVVVLVG"
     gene            complement(9507..10004)
                     /gene="rbfA"
                     /locus_tag="PKY13_07760"
     CDS             complement(9507..10004)
                     /gene="rbfA"
                     /locus_tag="PKY13_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT79866.1"
                     /translation="MGRPRQRAQARHQYNRTDRISETIREIVATELERLGDERVDMVT
                     VTGVTVDNDLNTAKVFYSALTAEAEGRVDEVAESLDEIRWPIQRVVNGAIRARKTPQI
                     VFLPDEVLAAALRIDDIIAHRVLPAGEDVDGTGAAPTPPPAGDGPATSAAQRPQVGDS
                     RPDPS"
     gene            complement(10133..>10377)
                     /locus_tag="PKY13_07765"
     CDS             complement(10133..>10377)
                     /locus_tag="PKY13_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOT79867.1"
                     /translation="IGKIAGCYVLNGKISRNSKVRFIREGTVIWKGEIQSLRRFKDDV
                     REVAAGFECGIGLSDYQDLRDGDIIETFDLVEVPRT"
BASE COUNT         1629 a         3405 c         3677 g         1666 t
ORIGIN      
        1 cgagggcgtc ccacagctcg atgacgaggt cgatcgacat cgcgttcaac cgctccgggc
       61 ggttcaacgt gatgcgggcg acgtggggcg cgggatgatc aatgaggacc aatcgatggc
      121 ctcggccacc ttcgctcagg ctcgctccct cgtccctcgg gagctgctta cgctgcggta
      181 cctcggcgtg gccgggagtg tctggcatcc gggggaagct agttggaacc ctcgtcctcc
      241 gaggccttct tgtcgccctt gccgcacttg gcgttctcgc agtcggtgac ggagagccgc
      301 gcgagcgccg tcaggccctc gttgctgtac aagcggatcc ggtacgtgcc ggcgtcatcg
      361 gcggtcacgg tcgtcacggc gtcgagtccg tagagtcctt cgtcgctgtc gtcgacgagc
      421 tcgatgccct cggccgactc cggactgaag ttcgccaccg gcgtgagcac aacgccggga
      481 tcgatgacgg cgaacgccat gtccgactgc ggtgactcgg cgcggatctc gaccttctgg
      541 cccgcttcga ggtcgagcag gtaatcggct gcggactcgt aggtggggat cacccggtcg
      601 acgacgtcac ccacctcgat cggctgttcg ctgagcttcg tcgtgagcgg aacggccggg
      661 atgctcgacg tgaacgccac cgtgatcgag tcggtggaca gcggtgccac gatgccgatg
      721 tagacgtcct cgtcggcggg gatctcgacg gagaacaccc cgggcgacgt ctcgggcttc
      781 atggccttct cgtccacgcc gtacgccgtc gggtcgggca actcgtcggg cttgccgccc
      841 gtcgctgcgg cgacctgctt catgagctcg gccatcaaca gcggggtgtt gtggctgacg
      901 gcgagcgagc tgtccgacag gttggtggag atgtccaccg tcacgacgtc ggtgtgatcg
      961 acggtgaagt cgagggttcg atcgtcgtcg gacgagggga tgaacagctg ctggtagtcc
     1021 gatgcccccg acaccgtcag cgtgccgctc ttcaccatgt cgtcggtctc gacccgtggc
     1081 accgccggat actcgtcgtc gcccttcccg gcgaggatgc gggcaatggc ctcgtcgacg
     1141 tccccaccgg agagcacgag tgcgaactcg tcatcggcga acgagagtcc ggagatgccg
     1201 atcaggtggc cggcggcgtc gaacaacggc ccaccggact gcccaccagc gatggacgcg
     1261 tcggtctgga agtaggtctg ctcgaacgcc ttcaccttcc gagtccggct gacgatgccg
     1321 ctggcgatcg ttgcctcgag gtcctcggga tcgtcggtgc tgacctcgcc ggggaacccg
     1381 acgagataga cgtcgtcgcc tcgctcgacc gcggcctcgg ggtcgaccgg caggggcgac
     1441 gggcccttct tgccgtcgaa cgggccgacc aacgcgatgt cggcgccggc atcgacgccc
     1501 aagatcggga gggcgtcgta gcgctcaccg cccacggtga cgtcggccgt ggcgaacgga
     1561 tcgaccacgt gggcgttggt gaggacgtag cggtcaccgt cgcgctcgat gaccacgccg
     1621 gtgccggaag cgtcctcgtt ctcgacgtac gccacggacc cctcggcgca acgcagcagc
     1681 gccttcgctg ccttctcgcc ggtcttggga gcgcaggcag cgggagcggt ggttgtggtc
     1741 gtcgacgacg acgacttgcc ggaggtacac gccacggaac cgacgaggcc gatcagaacg
     1801 agcgccgctc cggcgcggcg aaggtgagtg cggggaagca tgctgccgac gctatcggga
     1861 ctcggtgaaa accgatcacc tgctccccaa cgggacctcg gtcaggtgtc gggcccgacc
     1921 agcgacgtga cccgggagcc ggagaagacc tgaccggcga cgcgccggac atcgtcgtgg
     1981 gtcacggcct cgacggcatc gagcgactcg cggatcggga tgtggcgccc gtgggtgagc
     2041 accgccgagc cgaggcgact catccgcgag gacgaatcct cgagggccat caacatcgag
     2101 ccgctgaggt agcccttcgc gacggcgagt tcctcgtcat cgatgccgtc ggcgacgatc
     2161 gcggcgatcg tctcgtcgat gatggtcgcc acctcgctca ggtgctcagg acccgtggcg
     2221 cagtacacgg tctgggtgcc ggcatcgagg ttggacgccg ccatcgacga caccgagtag
     2281 gcgaggcccc gacgttctcg aatctcctcg tggagtcgac tcgagggacc gtcgccgagg
     2341 gcgtggttca tcaccccgag tggcacccga tcaccatcga gcgccgtgac accacgccag
     2401 ccgagggcga ggtgcacctg ttcgatcggc cgccgcgcct ccaccagccc cgtcatcgac
     2461 gagcccggag gaacccggtc gggtcgctcg ccgtcgaccg ccccgtcgaa ccgtgcgtcg
     2521 atcgccgcgg caacgaggtc gtggtcgact gctccggcaa cggagaacac gaggttcgac
     2581 ggccggtacc accgatggtg gaagccggcg atctcatcgg ccgtcatccg ctcgagggtg
     2641 tcgggatcgc cgagggtctc gcgaccgagt gagtggccga cgaacatggc ctccgccagg
     2701 ttcatgaaga ccacgtcgtc ggggctgtcg agcgctccgt cgagctcgcc caagatgacg
     2761 ttgcgttcga tctcgacgtc ggccgggtcg aaccgggggc tggcgatcac ctcgcacaac
     2821 aggtcggtcg cgaactgcgc gtgggcagcc ggaacccgga catagaacac cgtcgactca
     2881 cggaccgtgt aggcgttgaa gtcgccaccg acggcgtcga cggcgcggtt gatctcacgg
     2941 gcagtgcgtc gcatcgtgcc cttgaacagc aggtgttcga ggaagtgcga cgccccggcg
     3001 agctcggacg gctcgtcgcg gcttcccact cccacgaaac atgcgatcgc gaccgactgg
     3061 ctgtgcgcca tggggtcggt cgcgacccga acgcccgacg acagcaccga cacgcggaca
     3121 ccggcgtcga gcgcggcaac gtccaccgcc cctgccgaag gcaggagcag tggacgatcc
     3181 gacgtggtgg aacctgtcaa acgaggctcg tcatcggagc cactcggcac tgtcagcgcc
     3241 ggcgaggacg acgggcacgg ttgcggtcac gaccgccgcc gccaccggca ccggcaccga
     3301 cgcccttggc gacgggaccg agctcacctt gggcctcgcg ggtcttctcg tcctggaagt
     3361 cctcgaacga gacgagcacg gcgtcgcccg actccggcgg agccggctca tcgctcaccg
     3421 acgccgaagc ggcgccacgg ggacgaccac ccgaggccgc agcggacccg gcatcgtcct
     3481 ggtgacgcga tgcggtctcg gcgctgacac cctcgctggc cgctccagcg gcctcagctt
     3541 ttgggtcgga cccaccgtct gccgaatcgg aggcagtcgg tcggaggctg atcttgccgt
     3601 tgggatcgat gtcctcgacc cgcacctcga tctcgtcgcc gaggctgagt accgactcga
     3661 ccgagtcgat gcgcttgccg ccgccgagct tggagatgtg caccagacca tcacgccccg
     3721 gaaggatgtt gacgaaggca ccgaacttgg tgatgttgac caccttgccc tggtacacct
     3781 cgcccacacc cgcggtcggc gggttgagga tgaggcggat ctggcgctcg gcctcggtca
     3841 cgcatgccgg gtcgaccgca gcgatcgaca cgatgccgac catgccgtcg tcgtcgacgg
     3901 agatgttggc gccggtctcg gcctggatgg cgttgatgac cttgcccttc gggccgatga
     3961 tctcgccgat cttgtcggcg ggaatctcga agctcacgat cttgggagcg ttcggggaga
     4021 cctcggaacg gttctcgggg atggcggtgg ccatgacctc gaggatcgcg aggcgggctt
     4081 ccttggcctg ctcgagggcc gcggacaaga cgtcggcggg gatgccgtcg atcttggtgt
     4141 cgagctggag cgccgtcacg aactcggccg tgccggccac cttgaagtcc atgtcgccgt
     4201 aggcatcctc ggcgccgagg atgtcggtga gggtcgtgta cttgccttcg gcgaacacga
     4261 ggcccatggc gatgccggcg accggcgccc gcaggggcac accggcgtcc atcatcgaca
     4321 gggtcgagcc gcagaccgac gccatcgacg tcgagccgtt ggaggccaac acgtcggaca
     4381 cgcagcggat cacgtagggg aactcggcca tcggcgggac gaccggcagc agggcgcgct
     4441 cggcgagcag gccatggccg atctcacgac gcttcggccc acgcatgaag ccggtctcac
     4501 ccgtcgagaa cggcgggaag ttgtagtggt gcatgtagcg cttcgacgtc agcggcgtga
     4561 tgccgtcgat catctgatcc atcttgccca tggcgagggt ggccacgttg aggacctggg
     4621 tctcgccacg ctggaacagg cccgagccgt gcgcggtggg cacgtagccg acctcggccg
     4681 agagcgggcg gaggtcgcgg gtgccgcggc cgtcgatgcg ggcaccgtcg ttgacgatgc
     4741 ggctgcggac ggtggccttg gtgagcgact tgaacgccgc cttgagctgc ttgagcgcca
     4801 gtgcggcgtc gccgccggcg aactggtcga tgagcgcacc gacggtctcg tcacgaaggg
     4861 cgccttcggc tgcggtgcga tcgtgcttgt cggcgatcgt catgatctcg gagatcttgg
     4921 cgccggcagc gtcggacacg gccgagtact gctcgtcggt gtagtcgacc tgcaccggga
     4981 aatccatcac ggacttcttg ccgccggatg cctcgatcag ctggttctga agggcgatgg
     5041 cttccttgat ccactgcttg gaggcctgga ggccggcagc gagggcggct tcgtcgacct
     5101 tgggggcgcc gtcgctgtag aactcccagc tgttctcggt gccaccggcc tcgaccatca
     5161 tcacggcaac atcgccgtcg gcgagctcgc gcccggcgac gaccatctcg aagacggagg
     5221 tctcgccctc ctggtaggtc gggtgtgcga cccattcgcc gccgacgtcg taggcgatgc
     5281 gcacggcgcc gatggggccg tcgaagggaa cgtcggagat catcagcgct gcggaggcgg
     5341 cgttgatggc cagtacgtcg tgggggttct ccatgtcggc ggcgatgacg gtgccgacga
     5401 tgtggacctc gttgcggaag ccctcgggga acgacgggcg cagcggccgg tcgatgaggc
     5461 ggcaggtcag gatcgcctgg tcggaggact tgccctcacg ccggaagaac gagccgggga
     5521 tcttgcccgc agcgtacgag cgctcttcga tgtcgacggt cagggggaag aagtcgatgc
     5581 cgtcacgcac gcccttggcg gcggtggcgg tcacgaggat ctgggtgtca ccgatcgcgg
     5641 cgagcacggc gccgccggcg agcgaagcaa gcttgcccgt ctcgaacgac atggtcttgg
     5701 tctcgtctcc accaacggtg gattcgacac gaatggcgtc tgccattgtg gtctcactct
     5761 ctgtctggcg gacgccccga gtgggcatgg tccgcatctg tgagagatgt gtgtggttga
     5821 ccagttccca gttgtcgttg tccgaactcg ctgtgcccac tcgtcgatgg cgatcggacg
     5881 gggtgaggcg agcggtggct cgggcagcgg cgaccaggca ctgatcggca tggtcacatc
     5941 tctgggtgtt caggctaccc cacatgtggc ccaagcgtgg ggagccccgg aaaacgacga
     6001 actcccgacg tgaaacccgc aactggcggg ttccccgtcg ggagaacgat acgagtttcg
     6061 tcagcgacgc aggcccagct cggccacgat ggcgcggtag cgctcgatgt cgatctcctt
     6121 gaggtagtcg aggaaacgac ggcgacggcc gaccagcatg aggaggccac gacgggagtg
     6181 gtggtccttc ttgtggtcct tgaggtgggc ggtcaggtgg ttgatgcggt cggtgaggag
     6241 cgcgatctgc acctcgggag aaccggtgtc gcccttgccg cgggcgtgct tggcgatcgt
     6301 gtcggccttg gccggaaggt tggcgggggg acgtgacatt gggagtctcc tgaggtgggt
     6361 atcggacccg gcctcccaca cgcacagcgc agagtgggac gcacaaattg accggtgaac
     6421 cttaccagag ccgaccacca tctcccgaac ctcgccgcaa atgtgtcgca gcgcgatcgc
     6481 gagaccgtca acctcgcggc gcgtttcgcc gggcggcagt caggcccggc ggtcatgccg
     6541 cggcgctcaa cgcggcgatc agtccgcgat ggtccagcgg aacttggggc gacggtgacg
     6601 accggcaacg gtggtgacgt tgctcgccgg cgtgagctcg aagatgcgag cgtcggggcg
     6661 gtagagcgcc cgctcgatgt cggagagcgt cggctccttg gccgtcactc ccccacggcg
     6721 gatggccgca cggcaggcct cgccgagggt gcccggcagg agctgggcga cgatgccggg
     6781 caacgtctcg ggatcacgca ggagccacga gtggccaccg cccttgatgg tgatgagggt
     6841 gccctccgtc cgctcggccg cctcgcgggc cgtgcggaac ggcaccgcga ggtcacggtc
     6901 accgtgcatc acgtacaccg ggacgtcctc gtgtgcgagc tcgttgaggg cgtaccgact
     6961 cgaccgggac cgaaggatcg acaccaccgg gcccaacagt cgccacgggt ggacgatgtc
     7021 accgacgatc gtcggcaggg cgagccgcag gaacttgatc gcctgccggg gatcacgcca
     7081 gacgggcagg atcgtgatgg cgtcgacggt gagggccgcg cccacgacac ccacgagcgg
     7141 cggagccaga cggaacgcgt agaccatctt gtcccacgtg tcgccgacga tcgcgtcgat
     7201 gagcatcacg gcgatcgtgc gctccggacg caccgcagcg agttgggtga cgagacgacc
     7261 ccccatggag tgtccggcga ggatgcactg ctcgatgccg aggtcgtcga tgatgcggcc
     7321 gagcagctcg ctgtaggcct tgaggtcggc gccgtcggcg gggagcccgc gggtgccgcc
     7381 gtgcccggcg gtgtcgacgg cgatgacctt gaaccccatc gacacgagac gggacaacgt
     7441 ctgcgcgtac aggaacccct cgacggagaa cccgtgcacg accacgagcg ggatgccggc
     7501 accggcgacg gtgatgccga ccgtgtgccc gtcggagatc gtgatgcggt gccgtgccag
     7561 gcgcggggcg gccacgggaa cgccgtcgcc gggcacaccc tcggggaccg ggccgggaat
     7621 cggctcgacg acgtggctca tcgcggcctc aggagttcgc tcgacgcctg gcagtcgatg
     7681 gccagctgcg cggtcaattc ctcgatggtg tcgaacttcc ggtcgccccg gagtcggtgc
     7741 tcgaaatgga ccctcacgtc ttcaccgtac agatccccct cccaatcgag gcagtgcacc
     7801 tcgagcagag atgccggctg gtcgtcgtag aaggtcggcc gggtacccag gtagatcgcg
     7861 gacggcaacc gctcccccgt cggtcgttcg aggtgaccgg cgtagatgcc gtcggacggc
     7921 agcaggatct cgctccggat ggcgacgttg gcggtcggga acccgatggt gcggcctcgt
     7981 ttgtcgccgt cgacgacggg accccgcatc tcgtgcgggc gcccgagcat cgcgttggcg
     8041 tcatcgagac ggccctcgac gagcgcccgg cgaatcgccg tcgacgacac ctggagctcg
     8101 ggacgggcgt cggtccagtc gaggccgacg agatggtggc cgatgacctc gaagccgagc
     8161 tccgcgccca tctcctggag catcgcgacg ttgccggaac gtcggtgtcc gaaatggaag
     8221 tcttcgccga ccaccacgac cttcgcgccg aggcccccga cgagcacgtc acgcacgaag
     8281 tgctccgctg tttcgcgggc acgctcctcg tcgaagtgca cgacgagcgt ttggtcgatg
     8341 ccggtctcgc cgagccgctc gagcttctgg tcgaggtcgc acaggagcaa cggcgcgctc
     8401 tcggggcgga gcacctgggc ggggtggcgg tcgaacgtca cgaccgtggt ggtgagcccg
     8461 gccgctgcgg cccggtcgag cacggaactg atcagtgcct ggtggccgag gtggacaccg
     8521 tcgtagaacc cgatgctcac gacgctgccg ggacccggcg cggcgagctc ggtgaggtct
     8581 ctggcgatgc gcatcgcggc cgaacctacc cgaccagtac cacgaccggc ttgacccgac
     8641 cgtcaccgaa cggttcgtac atggccagca gctccccggt cggcgacagg atcggccacg
     8701 gtccggtgcc ggcgacgccc aacgcggcgc tgtccagcac ggcgccgaca cggatcgcgg
     8761 cttcggtcgc ctcgtcgacg cggacgatcg ggaagccgcg aaccgcctcc gccatcggga
     8821 tcaccgtcac gtcctcgagg ctggtcgcat cggcgagggt gaacgctccg accgacgtgc
     8881 ggcggagctt gcgcaggtgc gccccaccgc cgagggcatg tccgagatcg gcggcgagga
     8941 cccgcacgta ggtgcccgac gaacagtcga cgaacgcccg gtagacgagc gggtcgtcgg
     9001 tcggctcgac gtcgtagcgg tacacggtga ccggacgcgg cgggcgatcg acctcgatgc
     9061 ccttgcgcgc aagttcgtgg agccgcttcc cgtcgatctt gaccgccgac accatcggcg
     9121 gcacctgttg gatgtcgccg gtgaactgcg ccgtcgcggc cgtcgcctcg gccggatcga
     9181 cagtcatgtc gtaggtcgcc gtgacctcgc cggcggcgtc cagcgtgttc gtctcggtgc
     9241 cgaacacgat ctcgcactcg taggacttcg gcagcaactg caggaaccgc agcagcttcg
     9301 tcgcccggcc gacccccagg accagcacac cggtcgcgtc gggatcgagc gtgccggagt
     9361 gcccgacctt gcgggttccg aggatgcccc gggccttcgc aaccacgtcg tggctcgtcc
     9421 agccggcgtc cttgtcgacg atcgctaccc cgtggatggg tccgtcgccg gagtccttgc
     9481 cccttcgtcc catcagaacg cgcctgtcac gagggatcgg ggcgcgagtc gcccacctgc
     9541 ggacgctgcg ccgccgacgt cgccgggccg tcccccgccg ggggcggggt gggtgcggca
     9601 ccggtgccgt cgacgtcctc gccggcgggc aggacccggt gggcgatgat gtcgtcgatg
     9661 cggagcgccg cggcgagcac ctcgtcgggc aggaacacga tctgtggcgt cttgcgggcc
     9721 cgaatggcgc cgttgacgac ccgttggatc ggccaacgga tctcgtcgag tgattcggcg
     9781 acctcgtcga cgcgtccctc ggcctcggcg gtgagggccg agtagaagac cttggcggtg
     9841 ttgaggtcgt tgtcgacggt cacgccggtg acggtgacca tgtcgacccg ctcgtcgccg
     9901 agccgttcga gctcggtcgc gacgatctca cggatcgtct ccgagatgcg atcggtgcgg
     9961 ttgtactggt gacgggcctg ggcccgctgg cgtgggcgcc ccatggagac caccccttcc
    10021 ttcgttcgaa tttggccgcg cccggccgcc gacggcaccc acggcgtggg cacggtcggc
    10081 ggcggaacgg gacgaacagg tgtaccaggt cgggagaccg ggcacgccgg gattaggtcc
    10141 gtgggacctc gacgagatcg aaggtctcga tgatgtcgcc gtcgcggagg tcctggtagt
    10201 cgctgagacc gataccgcac tcgaacccgg cggccacttc gcggacatcg tccttgaatc
    10261 ggcgcagcga ctggatctcg cccttccaga tgaccgtgcc ttcacgaatg aagcggacct
    10321 tggagttgcg gctgatcttg ccgttgagca cgtagcagcc ggcgatcttg ccgatct
//
LOCUS       DAOSOA010000093        10284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6505_length_10284_cov_5.306677, whole genome shotgun sequence.
ACCESSION   DAOSOA010000093 DAOSOA010000000
VERSION     DAOSOA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6505_length_10284_cov_5.306677"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKY13_07770"
     CDS             <1..782
                     /locus_tag="PKY13_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79868.1"
                     /translation="GSTKKFHTDFGSGTWEGAPIGIPYNVVGAGQPKVPISFYYPEDS
                     DPGPYPLAYDAPVEGGADSDGDRHVLTVDSATCTLYEVFDARRGASPTAAWTAGSGAV
                     FDLNSNAMRPDTHTSADAAGLPILPGLVRYDEVASGHIDHAIRFTANRTSKSYIWPAK
                     HQAGSTTDPNVPPMGAWFRLRADYPTAGLSNEAKVIIEAMKTHGIILADNGTSFMLSG
                     APDERWNNPALTPIWNITGADIVAVDTSGLMVSPNSGQVRQ"
     gene            complement(831..1775)
                     /locus_tag="PKY13_07775"
     CDS             complement(831..1775)
                     /locus_tag="PKY13_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOT79869.1"
                     /translation="MRNDALRVVSMLAASSNNVFTRTQAASAGLDRRQVRRLIDTDVL
                     HEPWPGILIALPPGGDPTWRQLCTGSTRKGAVASHRAAGRLHRLDGLESCDLIETSAE
                     GGSPRLAGVVQHQASSLPRCDLVTVQGVRCTGNARTLTDLGSVASIETVERAFDDAVR
                     RGTNPRWLRETAERLRRPGVAGPLVLLSLVDAFERRGTVRGSWFERLVELALQHPELD
                     DLVIQHRLERSDGTHVATFDLALPDAKLAIEAHSRKHHFTEADESSDEFRDHEASVLD
                     WDVMYIGYIATRRPEQALRMVLDRARSRRALLAERSSS"
     gene            complement(1883..3187)
                     /locus_tag="PKY13_07780"
     CDS             complement(1883..3187)
                     /locus_tag="PKY13_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOT79870.1"
                     /translation="MGQALDIAPATGKLGILTPGMGAVASTAFAGIVATRMGLAAPIG
                     SLTQMAHIRLGERSEGRNPLIREFVPLASLDDIVFGGWDPISPNALEAARTSGVLSNE
                     DLAPLSAELEGVVAMDAVFDQRWVKRLDGVRVKTGATKWDLVEQLVADIERFRSENGC
                     DRLVMIWCGSTEAYQEPTDVHQTVAAFEQGLRDSDESISPSQMYAYAAISSGVPFGNG
                     APNLAIDIPCMDELAAANNVPLTGKDFKTGQTLMKTILAPGFKARMLGLRGWYSTNIL
                     GNRDGEVLDDPENFKTKEMSKLGVLHEILQPEVYPDLYGNIDHVVRINYYPPRGDNKE
                     GWDAIDIFGWMGYPMQIKVDFLCRDSILAAPIVLDLALFLDLAQRAGQSGVQEWLSFY
                     WKAPQTAKDHDPEHDLFIQQTKLKNTLREWMGEPAVTHSEAG"
     gene            3308..4246
                     /locus_tag="PKY13_07785"
     CDS             3308..4246
                     /locus_tag="PKY13_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOT79871.1"
                     /translation="MGHENGIDRFAGIELTEIASGLQFPEGPVALSDGSVLVVEIQGR
                     CLTRIGPDGARETVAEFAGAPNGAAIGPDGAVYLCDNGGYFDYDDTSGVNVPSSGASG
                     WRKGSIQRVDLATGETEELYTEADGHRLLAPNDLVFDAHGGFWFSDHGVHAHDTDVGA
                     AVLYARADGSTIRGVISGLDATNGVGLSPDGTRLYVVETYRGRVLAWDVTGPGEVADP
                     GDPQVMHDAGGTAMFDSLAVDADGSVCVATLLTGGVSVVSPEGSVEFLATGDPLTTNI
                     CFGGDDLRTAWITSSGTGRLLRCRWPVPGLRLEHTA"
     gene            4298..5614
                     /gene="rimO"
                     /locus_tag="PKY13_07790"
     CDS             4298..5614
                     /gene="rimO"
                     /locus_tag="PKY13_07790"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOT79872.1"
                     /translation="MTRTYWIETLGCPKNQVDSDKIGGKLVADGYEAASDPSSADLVV
                     VNTCAFVEEARQESIDTVLALSDDRADGASVVVTGCMAERYGDELAAALPEVDTVAGF
                     GKAFAEPASSPRRSLIPVTSAPVPTFDLLNLPRPASASPWAYVKIAEGCDRNCGFCAI
                     PSFRGPQRSRSIDDIAAEVEALGAREIVLVAQDLAAFGRDQGVGERAIVPLVRRITDL
                     VDRVRLLYLYPSDLTEELIEAMLATGVPYFDLSLQHVSPPLMRRMRRWGNGDKFLERI
                     ERIRTLEPDAAFRSNFIVGYPGETEADHDALLRFVEAAHLDWCGFFGFSDEEGTYAHT
                     LDGHVAPDLMDDRLAELRELQDGITASRRDALIGREVTVLVDAPGEGRTFREAPEIDG
                     IVTVPDTLEPGRFHTVRVIDAQGPDLVAEPVLEPSAAGPSPRGADS"
     gene            5611..6189
                     /locus_tag="PKY13_07795"
     CDS             5611..6189
                     /locus_tag="PKY13_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT79873.1"
                     /translation="MTDLKAPTTYGPSAILTPANLVTLVRLAVAPVAFAMMLTDANQS
                     SWPLVALWFALSSSDMIDGNLARKYGATRSGAFLDPLADKVLTMGGIAAMCSQGRFPW
                     IALVLIAVREAAVSGFRAVYVRRGLAVPASKLAKWKAFVQMGAVGWVTLPWTNEYEWL
                     STTTLWVGVVIGLISGVQYFVAGGRAATTMAR"
     gene            6358..7263
                     /locus_tag="PKY13_07800"
     CDS             6358..7263
                     /locus_tag="PKY13_07800"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOT79874.1"
                     /translation="MRQRRWIVTGVALMALVATAAGCGSDDESKADAKDQATSTASST
                     TAAGGSSTTAAGSVNTRPPPEESDPSKEALRILVSNDDGIGAKGIDALVMALRMLPNT
                     EVTVVAPKDNQSGTGSKTTEGEVAVAEATTISGYNGTAVSGYPSDAVIYALDKGGMPT
                     RPHVVITGINEGANIGPLVNISGTVGAARAAAQRGIPALASSQGLATEPDYPSGVKYV
                     LEWLAEHRAALLDGTAPVTVTSLNIPTCASGSIRGVVEVPTAADAGGRELFTSNCEST
                     TPAPTDDVDGFSNGYATFSTVPNAA"
     gene            7415..8677
                     /locus_tag="PKY13_07805"
     CDS             7415..8677
                     /locus_tag="PKY13_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOT79875.1"
                     /translation="MRADVVAIGTELLLGQIIDTNSSWIGEQLALTGIDTHFQTKVGD
                     NVARIVEAIHQALERSDAVICCGGLGPTQDDLTRQAIAQVLGVELVFDADKADLIRHM
                     FGSRGRDMPDNNLLQAFRPEGAHFIEQQPGTAPGLVAPLTVTDADGNTVEKVIYAVPG
                     VPWEMREMVEGTVLPDLKARSGDDFVIRSRVLRTWGQSESGLAEMLADRIVELDELGN
                     PTLAFLASGIEGLKVRVTAKAATDAEATDLLDEEEARLRRVLGELVFGVDDDTMESVV
                     LDLLAERGFTLSVAESVTGGLVAARLVGVPGASANFVGGVVAYDSRVKFNVLGVPEGP
                     VITLEAAEAMASGVRKLLGSTVGIGITGVAGPDAAEGRPPGTVCVAVDLDGDVSSMEY
                     RLPGRRNQVREFSCITALGMLRSKLTPG"
     gene            8749..9534
                     /locus_tag="PKY13_07810"
     CDS             8749..9534
                     /locus_tag="PKY13_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT79876.1"
                     /translation="MKRALFLPPFDDLADVRVLAGLAAEAESAGWDGVFLWDHVLRPD
                     PPRPVADPWIALAALAMRTERVRLGTMVTPLARRRPQKLAREVATLDHLSGGRVTLGL
                     GLGVDTGRELSGFGEPVIGAAERGDLLDEGLPLLRAMLRGERIDHHGRFFTADGVTLV
                     PAALQRPVPIWMAVRTSNRRPARRAATVDGIFPIELSPTEVGELLGVIADERGGLDRF
                     DVVALASSGFPQAEWEAAGATWWFADLEPGATAVDVRALIHGS"
     gene            9712..>10284
                     /gene="recA"
                     /locus_tag="PKY13_07815"
     CDS             9712..>10284
                     /gene="recA"
                     /locus_tag="PKY13_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT79877.1"
                     /translation="MERDKALDGALAQIEKQFGKGSVMRMGEKTSMKIGTISTGALAL
                     DIALGVGGLPRGRVTEIYGPESSGKSTLAMHVVAEAQRNGGTCAYIDAEHAMDPMYAA
                     KIGVDVDQLLISQPDTGEQALEITDMLIRSGALDVVVIDSVAALTPRAEIEGDMGDTH
                     VGLQARLMSQALRKLTANLNKSDTICIFINQ"
BASE COUNT         1609 a         3471 c         3582 g         1622 t
ORIGIN      
        1 tcggttcgac caagaagttc cacaccgact tcgggtcggg cacgtgggag ggcgcaccga
       61 tcggcatccc ctacaacgtg gtcggtgccg gccaaccgaa ggtgccgatc agcttctact
      121 accccgagga cagtgatccc gggccctacc cgctcgccta cgacgcgccc gtcgagggtg
      181 gcgccgactc cgacggcgac cgccacgtcc tcacggtcga cagtgccacg tgcacgctgt
      241 acgaggtgtt cgatgcccgt cgaggcgcct cacccactgc ggcctggacc gccggctcgg
      301 gagcggtgtt cgacctgaac tcgaacgcga tgcgtcccga cacccacacg tcggccgatg
      361 cggcgggcct gcccatcctc cccggtctcg tgcgctacga cgaggtcgcc tcgggccaca
      421 tcgaccacgc catccgcttc acggccaacc gcacctcgaa gtcctacatc tggccggcca
      481 agcaccaggc agggagcacg accgacccaa acgtgccgcc catgggagcg tggttccgcc
      541 tccgcgccga ctacccgaca gcgggcctct cgaacgaggc gaaggtcatc atcgaggcga
      601 tgaagaccca cggcatcatc ctcgccgaca acggtacgag cttcatgctg agcggcgccc
      661 ccgacgagcg ctggaacaac cccgccctca ccccgatctg gaacatcacg ggcgccgaca
      721 tcgtcgccgt cgatacctca ggcctgatgg tcagccccaa cagcggccag gtccgtcagt
      781 agctcgaggg aacgtccgaa gttcgtcgtg tcggtaccgg tgccgctcgg tcacgacgaa
      841 cttcgttccg caagcaacgc ccgtcggctt cgggcacggt cgagcaccat tcggagcgcc
      901 tgctccgggc gacgggttgc gatgtagccg atgtacatca cgtcccagtc gaggacgctc
      961 gcctcgtggt cccggaactc gtcgctcgac tcatcggctt cggtgaagtg gtgcttgcga
     1021 ctgtgcgcct cgatggcgag cttggcatcc ggaagcgcga ggtcgaaggt cgccacgtgg
     1081 gtgccatcgg aacgctcgag gcggtgttgg atcaccaggt catcgagttc gggatgctgc
     1141 agcgcgagtt cgaccaacct ctcgaaccag gaaccccgga cggttccacg gcgttcgaac
     1201 gcgtcgacca acgacagcag gaccagtgga ccggcgacgc ccggccgccg gagtcgctcc
     1261 gcggtctcgc gaagccaccg cggattcgtc ccccggcgaa cggcgtcgtc gaaggcccgc
     1321 tcgacggtct cgatcgacgc caccgagccg aggtcggtca gggtccgagc gttaccggtg
     1381 caacgaactc cctgcacggt cacgagatcg catcgaggga ggctgctggc ctggtgctgc
     1441 acgaccccgg ccagccgggg cgagccaccc tcggcggagg tctcgatgag gtcgcagctc
     1501 tcgagtccgt cgaggcgatg gagacggccg gcagcgcggt gcgacgcgac cgcgcccttg
     1561 cgggtcgacc cggtgcacag ttggcgccag gtcgggtctc cgccaggtgg gagcgcgatc
     1621 aggatgccgg gccagggctc gtggaggacg tcggtgtcga tcaagcgccg gacctggcgg
     1681 cggtcgagtc cggcggaagc agcctgagtg cgggtgaaga cgttgttcga agaggctgcg
     1741 agcatcgaga caacgcgcaa ggcgtcgttt cgcatggatc gatatccaac acgacacccc
     1801 cgacaccgag aagttcgtcg tcagcgggct gcgccagcgc gcagacgacg aacttccggc
     1861 tcgggtggga gctggcggcg gctcagccgg cttcggagtg ggtgacggcg ggttcgccca
     1921 tccactcgcg gagggtgttc ttgagcttgg tctgctggat gaacagatcg tgctcgggat
     1981 cgtggtcctt cgccgtctgc ggggccttcc agtagaagct cagccactcc tgcacgccgg
     2041 actggccggc gcgctgggcg agatcgagga acagcgccag gtccaagacg atcggcgccg
     2101 cgaggatcga gtcgcggcac aggaagtcga ccttgatctg catcgggtac cccatccatc
     2161 cgaagatgtc gatggcgtcc cagccctcct tgttgtcgcc ccgaggcggg tagtagttga
     2221 tgcgcacgac gtggtcgatg ttgccgtaga ggtcggggta cacctcgggc tgcaggatct
     2281 cgtggaggac accgagcttc gacatttcct tggtcttgaa gttctcggga tcgtcgagca
     2341 cctcaccgtc gcggttgccc aagatgttgg tcgagtacca cccgcgcagg ccgagcatcc
     2401 gggccttgaa gccgggcgcc aggatcgtct tcatgagtgt ctggccggtc ttgaagtcct
     2461 tgccggtgag cggcacgttg ttggccgcgg cgagctcgtc catgcagggg atgtcgatgg
     2521 cgaggttggg ggcaccgttg ccgaacggta cgccggagct gatcgccgcg tacgcgtaca
     2581 tctgagaagg ggagatgctc tcgtcgctgt cacggaggcc ctgctcgaac gctgcgacgg
     2641 tctggtggac gtcggtgggc tcctggtacg cctcggtcga cccacaccag atcatgacga
     2701 gccggtcgca gccgttctcg gagcggaacc gctcgatgtc ggcgacgagc tgctcgacga
     2761 ggtcccactt cgtggcgccg gtcttcaccc ggacgccatc gagtcgcttg acccagcgct
     2821 ggtcgaacac cgcatccatg gcgacgacac cctcgagctc ggccgacagg ggagcgaggt
     2881 cctcgttgct gagcacaccc gacgtgcgag cggcctcgag cgcgttgggc gagatcgggt
     2941 cccagccgcc gaacacgatg tcgtcgagcg acgccagggg gacgaactcg cgaatgagcg
     3001 ggttgcggcc ctcactgcgt tcgccgagac ggatgtgggc catctgcgtg agcgaaccga
     3061 tcggtgcggc gaggcccata cgagtggcca cgatgccggc gaaggcggtc gaggcgacgg
     3121 cgcccatgcc gggcgtgagg atgccgagct tgccggtcgc cggcgcgatg tcgagtgctt
     3181 gacccacgtg ttgtgactct ctttcgaatg gaggctgtcg gaacggacgc tgacagacaa
     3241 cgaacggtct agcttcgcac tcgcgccggc cgacagttgg tatggcgaac gaacatcggg
     3301 ggatgacatg ggtcacgaga acggaatcga tcgtttcgcg gggatcgagc tgaccgagat
     3361 tgcgtcgggg ctgcagttcc cggagggccc ggtggcgctg tccgacggca gcgtgctcgt
     3421 cgtcgagatc cagggtcgct gcctcacgag gatcgggccc gacggcgccc gggaaaccgt
     3481 ggcggagttc gccggggccc ccaacggcgc cgcgatcggc cccgacggag ccgtctacct
     3541 gtgcgacaac ggcggctact tcgactacga cgacacgagc ggcgtgaacg tgccgtcgtc
     3601 gggtgcgtcg ggctggcgga aggggtcgat ccaacgcgtc gacctggcga ccggcgagac
     3661 cgaggagctg tacaccgagg ccgacggcca ccggctcctg gcacccaatg acctcgtgtt
     3721 cgacgctcac ggcggcttct ggttcagcga ccacggcgtg cacgcccacg acaccgacgt
     3781 gggcgcagcg gtgctgtacg cccgggcgga cggttcgacg atccgcggtg tcatcagtgg
     3841 cctcgacgca acgaacggcg tcggcctgtc gcccgacgga acccgtctct acgtcgtcga
     3901 gacctaccgg ggtcgagtcc tcgcgtggga cgtgacgggc cccggcgagg tggcggaccc
     3961 cggcgatccc caggtcatgc acgatgcggg cgggaccgcg atgttcgact cgctcgccgt
     4021 cgacgccgac ggctcggtgt gcgtggcgac gctgctcacc ggcggggtca gtgtcgtgtc
     4081 gccggaggga tcggtcgagt tcctcgcgac cggtgatccg ctcaccacga acatctgctt
     4141 cggcggtgac gacctccgta cggcctggat cacctcgtcg ggtacgggtc ggctgctccg
     4201 ttgccggtgg cccgtccccg ggctccgact ggagcacact gcctgacccg tcacggcggc
     4261 gctcggcgcg gtcgaccgcc tccgctaccc ttgccccgtg acgcgcacgt actggatcga
     4321 gacgctgggc tgtcccaaga accaggtcga ttcggacaag atcggcggca agctcgtggc
     4381 cgacgggtac gaagcggcgt ccgatccgtc gtcggccgac ctcgtggtcg tcaacacgtg
     4441 tgccttcgtc gaggaggcgc ggcaggagtc gatcgacacc gtcctcgcgc tgagcgacga
     4501 tcgggccgac ggcgccagcg tcgtcgtcac ggggtgcatg gccgagcgct acggcgacga
     4561 actcgcggcg gcactacccg aggtcgacac cgtcgccgga ttcggtaagg cgttcgccga
     4621 accggcatcg tcgccccgtc gctcgctgat tccggtcacg agcgcacccg tcccgacctt
     4681 cgacctgctc aatctcccgc gcccggcctc cgcgtcgccg tgggcgtacg tgaagatcgc
     4741 cgaggggtgc gaccgcaact gcggcttctg cgccatcccc agcttccgcg gacctcaacg
     4801 cagccgctcg atcgacgaca tcgccgccga ggtcgaggcg ctcggcgccc gcgagatcgt
     4861 cctggtcgcg caggacctgg cggcctttgg gcgtgatcag ggcgtcgggg aacgggcgat
     4921 cgtgccgctc gtgcgccgga tcaccgacct ggtcgaccgg gtccgcctgc tctacctgta
     4981 cccctccgac ctcaccgagg agctcatcga ggcgatgctc gccaccggtg tgccgtactt
     5041 cgacctgtcg ttgcagcacg tcagcccgcc gctcatgcga cgcatgcgcc ggtggggcaa
     5101 cggggacaag ttcctcgagc gcatcgaacg gatccgcacc ctcgaacccg acgccgcgtt
     5161 ccgatcgaac ttcatcgtcg gctaccccgg cgagaccgag gccgaccacg atgccctcct
     5221 ccggttcgtc gaggccgcgc atctcgactg gtgtggcttc ttcgggttct ccgacgagga
     5281 aggcacctac gcccacaccc tcgacggaca cgtcgccccc gacctgatgg acgaccgcct
     5341 ggccgaactc cgagaactgc aggacggcat cacagcgagc cgccgcgacg ccctgatcgg
     5401 ccgtgaggtc accgtgctgg tcgacgctcc gggtgagggt cggacgttcc gtgaagcccc
     5461 cgagatcgac ggcatcgtca ccgtccccga caccctcgag ccggggcggt tccacacggt
     5521 ccgagtcatc gacgcccagg gccccgacct cgtggcggag ccggtgctcg aaccgagcgc
     5581 ggcggggccg agcccgcgag gggccgactc gtgaccgacc tgaaggcgcc gacgacgtac
     5641 ggcccgtcgg ccatcctgac gccggccaac ctcgtcaccc tggtccgtct ggccgtcgcc
     5701 cccgtcgcgt tcgccatgat gctgaccgac gcgaaccaga gctcgtggcc actcgtcgcg
     5761 ctgtggttcg ccctgtcgag cagcgacatg atcgacggca acctggctcg caagtacggg
     5821 gcgacgcgtt cgggggcgtt cctcgacccg ctcgccgaca aggtgttgac gatgggcggc
     5881 atcgcagcga tgtgctccca gggtcggttc ccgtggatcg cgctggtgtt gatcgccgtg
     5941 cgcgaggccg ccgtgagcgg cttccgcgcc gtgtacgtgc gtcggggcct cgccgtaccg
     6001 gcgtccaagc tcgccaagtg gaaggcgttc gtgcagatgg gtgcggtggg ctgggtgacg
     6061 ctgccgtgga ccaacgagta cgagtggttg tcgacgacca ccctgtgggt cggcgtggtc
     6121 atcgggctga tcagcggcgt gcagtacttc gtcgccggcg ggcgggccgc gacgaccatg
     6181 gcccgctgag gccggtcgct ctccgcgttc ccggacggca ccgcaactcc gtcgtggtgt
     6241 cgtcgcggcg tcacccggcc gtcacctcgt cgttgctccg ccgtcacccg gcgatcggcc
     6301 atactcctac gggtccgagc cacatggggt ggtcggaccg atgtcgaggg agggccgatg
     6361 cggcaacgcc ggtggatcgt gacaggtgtg gcgctcatgg cgctcgtggc gacggcggcg
     6421 ggctgcgggt ccgacgacga gtcgaaggcc gacgccaagg accaagcgac ctcgaccgcg
     6481 tcctcgacga cggcggcggg tggatcgtcg accaccgcgg ccggttcggt caacacccgg
     6541 ccaccgccgg aggagagcga cccgtcgaag gaagcgctcc ggatcctcgt gagcaatgac
     6601 gacggcatcg gggcgaaggg catcgatgcc ctggtgatgg cactgcgcat gctcccgaac
     6661 accgaggtca ccgttgtggc tccgaaggac aaccagagcg gcaccggctc gaagacgacc
     6721 gagggcgagg ttgccgtcgc tgaggccacc acgatctcgg ggtacaacgg cactgcggtc
     6781 tccggatacc cgtcggacgc ggtcatctat gcgctcgaca agggcggtat gccgacgcgg
     6841 ccgcacgtcg tgatcacggg catcaacgaa ggggcgaaca tcggcccgct ggtgaacatc
     6901 tcgggcaccg tcggcgccgc ccgtgccgca gcccagcggg gcatcccggc cctggcgtcg
     6961 agccaggggt tggcgacgga gccggactac ccgtcgggtg tgaagtacgt gctcgagtgg
     7021 ctggccgagc accgagcggc ccttctcgac ggaaccgctc cggtgaccgt gacgagcctc
     7081 aacatcccca cgtgcgcgag cggttcgatc cgcggggtcg tcgaggtgcc gaccgcggcg
     7141 gacgccggag ggcgcgagct gttcacgagc aactgcgagt cgacgacgcc ggcgccgacc
     7201 gacgacgtcg acggcttcag caacggctac gccacgttct ccacggtccc gaacgcggcc
     7261 tgagccatct cgcccccagt tggccaggtg aacctgcccg aggtggcgga gctcacgtcg
     7321 atcgtcgacg acgtctgagg ccgtcgaccg tttcggctcg atcgatcatc cggagcggcg
     7381 gggggaggcg cgggggaggg ctagcctccg ccccatgcgc gctgacgtgg ttgccatcgg
     7441 caccgaactg ctcctcggcc agatcatcga cacgaactcg tcgtggatcg gtgagcagct
     7501 cgcgctcacc ggcatcgaca cgcacttcca gacgaaggtg ggcgacaacg tcgctcgcat
     7561 cgtcgaggcc atccaccagg cgctcgagcg cagcgacgcg gtcatctgtt gtggaggtct
     7621 cggccccacc caggacgatc tcacccgtca ggcgatcgcc caggtcctcg gggtcgagct
     7681 ggtcttcgac gcggacaagg ccgatctgat ccgccacatg ttcgggtcgc gtggccgtga
     7741 catgcccgac aacaacctgc tgcaggcgtt tcggcccgag ggcgcacact tcatcgagca
     7801 acagcccggc accgcgcccg gcctcgtcgc cccgctcacc gtgaccgatg ccgacggcaa
     7861 caccgtcgag aaggtcatct acgcggttcc cggtgtgccg tgggagatgc gagagatggt
     7921 cgaaggcacg gtgctgcccg acctcaaggc tcgcagcggc gacgacttcg tgatccgcag
     7981 ccgggtgttg cgaacgtggg gccagtccga gtccggcctc gccgagatgc tcgccgaccg
     8041 gatcgtcgag ctcgacgagc tgggcaaccc gaccctggcg ttcctggcgt cgggcatcga
     8101 gggcctgaag gtgcgcgtca ccgcaaaggc agcgaccgat gccgaggcga ccgacctgct
     8161 cgacgaggag gaggctcggc tccgccgggt gctcggtgag ctggtgttcg gggtcgacga
     8221 cgacacgatg gagtccgtgg tgctcgacct gctcgccgaa cggggcttca cactgtcggt
     8281 cgccgagtcg gtcaccggtg ggctcgtcgc cgctcgtctc gtcggcgtgc cgggagcgtc
     8341 ggcgaacttc gtcggagggg tcgtggccta cgactcgcgg gtcaagttca acgtcctcgg
     8401 ggtgcccgag ggtccggtca tcacgctgga ggccgcagag gcgatggcgt cgggcgtgcg
     8461 gaagctgctc ggctcgacgg tgggcatcgg catcaccggc gtggccggtc ccgacgcggc
     8521 cgagggtcgt ccgcccggca cggtgtgcgt cgccgtcgac ctcgacggcg acgtgtcctc
     8581 gatggagtac cggctcccgg gtcggcgcaa ccaggtgcgc gagttcagct gcatcacggc
     8641 gttgggtatg ttgcggtcga agctcacccc ggggtgagcc gggtccaggg ggtcgtcatg
     8701 cagcgggcat cgtcgtgaag cgggcactgt cgtgaagcgg gcactgtcgt gaagcgggcg
     8761 ctgttcctgc cgccgttcga cgacctggcg gacgtccgcg tgctcgccgg cctggccgcc
     8821 gaggccgagt cggcgggctg ggacggtgtg ttcctgtggg accacgtgct gcgtcccgat
     8881 ccaccccggc ccgtcgccga tccgtggatc gccctggccg ccctggccat gcgcaccgag
     8941 cgcgtgcgtc tcggcaccat ggtcacgccg ctcgctcgcc gtcggcccca gaaactggcc
     9001 cgagaagtgg cgacactcga tcatctgtcg ggcggacggg tgacgctcgg tctcggcctc
     9061 ggtgtggaca ccggccgcga gctctcggga ttcggcgagc cggtgatcgg tgccgctgag
     9121 cgcggcgatc tcctcgatga agggctgccg ttgctccggg ccatgctccg cggcgaacgc
     9181 atcgatcatc acggacggtt cttcaccgcg gatggcgtga cgctcgtccc tgccgcactg
     9241 caacggcccg tgccgatctg gatggcggtg cggacgtcga accgccgtcc ggctcggcgc
     9301 gccgccaccg tcgacgggat cttcccgatc gagctctcgc ccaccgaggt gggggagttg
     9361 ttgggcgtga tcgccgacga gcggggcggt ctcgatcgct tcgacgtcgt ggccctcgcg
     9421 tcgagcggct tcccgcaggc ggagtgggag gcggctggtg ccacgtggtg gttcgccgac
     9481 ctggagcccg gggcgacggc agtcgacgtc cgggcactca tccacggctc ctgatccgac
     9541 aactgcaaaa tctgtccgag ggtgcttgat cgaacagctg ttcgaaagta cggtgctgtc
     9601 attcgaaacg gcgccgctcc ggactcactg tcaggggtcg tcgttaggtt cagcaccaac
     9661 gtgatcgctg cacgatgcag cctcagatga cccagtgaag ggatgaatgc agtggagcga
     9721 gacaaagcac tcgacggagc gttggcacag atcgagaagc agttcggcaa gggctcggtc
     9781 atgcggatgg gcgagaagac gtccatgaag atcggcacga tctcgaccgg cgcgctggcg
     9841 ctcgacatcg ccctcggggt cggtggtctg ccccgtggtc gtgtcaccga gatctacggt
     9901 ccggagtcgt cgggcaagtc cacgctcgcc atgcacgtgg tggccgaggc gcagcgcaac
     9961 ggcggcacgt gcgcctacat cgacgccgag cacgccatgg accccatgta cgcggcgaag
    10021 atcggcgtcg acgtcgacca actcctcatt tcccagcccg acacgggcga gcaggcgctc
    10081 gagatcaccg acatgctcat ccgctcgggt gcgctcgacg tggtggtcat cgactcggtg
    10141 gcggcactca cgccccgggc cgagatcgag ggcgacatgg gcgacaccca cgtcggtctg
    10201 caggcacgtc tgatgtccca ggcgctgcgc aagctcaccg ccaacctcaa caagagcgac
    10261 accatctgca tcttcatcaa ccag
//
LOCUS       DAOSOA010000094        10209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6566_length_10209_cov_5.795056, whole genome shotgun sequence.
ACCESSION   DAOSOA010000094 DAOSOA010000000
VERSION     DAOSOA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6566_length_10209_cov_5.795056"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKY13_07820"
     CDS             <1..371
                     /locus_tag="PKY13_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79878.1"
                     /translation="QLAWQPFVKQVEMLPEAFGDVVDADQVDEIDRGRYTRNSDADYG
                     YFGAEPRIDVWWLWMGPTDASGYTWLFALPIVGLFAAAGYLLVRDRRIGQWDEATASS
                     ADRYQSTSAVSSDGSPTSSI"
     gene            complement(281..1015)
                     /locus_tag="PKY13_07825"
     CDS             complement(281..1015)
                     /locus_tag="PKY13_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79879.1"
                     /translation="MTSAHGTNRVHPTAVIGPGVELGGDNVIGPYAVLLGPARIGNGN
                     WIGPHTAIGTPGEMRGGDHPTAWDEPAVHGGVVIGDRNVIREFATVQAPAFGQTIIGD
                     DCYVMTKAHVPHDGVLSDGVTVSCGALIGGHGRIGANANLGLNSVLHQELVVGPGAML
                     GMGTIVTKAVPPYATVTGNPGRVRGVNWRGMQRVGCSDEAIEWLRALYVPDTKLDQIE
                     LVGEPSELTADVDWYRSALEAVASSH"
     gene            1068..2447
                     /locus_tag="PKY13_07830"
     CDS             1068..2447
                     /locus_tag="PKY13_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT79880.1"
                     /translation="MAPTSNALRFVIIGGGPAGTQAATVAARLGADVTLIERDIIGGA
                     AHLWDCIPSKAMIATGAAFGEFGEAAQMGLHAQTAQIDLDALRGRITGIVGRLEQSGR
                     RLLESQGVRIVMGTGRLVDEHTVEVTTPDGLTEELPADRILVSTGSRPRIPEFAHPDG
                     DRVLTTRDAYPPKEIPEHLIVIGSGVTGVEFVHMFLSLGSKVTLVVSRNQVLPQKDPE
                     VAAVLEDDFVRRGVKLLKGARATGVERTDDGVKVICDDGRVAVGSHALLAIGSIPNSE
                     GLGFAEVGVEMDGGYVSTTDHHLRTNLPNIYVAGDLSGKLPLSSVASMQGRKIAEHVM
                     GMHIVAHRHLDYEKAASAIFTEPEIADVGLAEADAFAEGRKVRVTKVPFATTAKALIN
                     NDSRGFVKLVSDPATGVVLGGSIVGQHAAELISVIAVAVTAGLRVNDLAESVLVHPAL
                     AEALMEAAE"
     gene            2444..2968
                     /locus_tag="PKY13_07835"
     CDS             2444..2968
                     /locus_tag="PKY13_07835"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT79881.1"
                     /translation="MSEGPWQQPPPTPWAPVDETVGRAGVIPPLWKRVVARVVDELLL
                     AIPRLALTLPFVTWADPPELNLPQWALALSVVIPLLYDFAFVAIWRATPGKLLFGIAV
                     CDSPDGGRASIQQAALRALVPSIGSALLLVMPTSEASSFLALITPVVYASVAWDPRRR
                     GLHDKAAGTIVLYR"
     gene            complement(3057..4811)
                     /locus_tag="PKY13_07840"
     CDS             complement(3057..4811)
                     /locus_tag="PKY13_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOT79882.1"
                     /translation="MLIANRGEIAVRVIRACKELGISTVAVYSELDRDALHVRLADEA
                     YALGGQTAAESYINTEKLIDVIQRSGAEAVHPGYGFFSENSDFAKAITDMGVKFIGPR
                     PDAIDVMGDKISARLAAERVEVYGVPGTTELITDPQQIIDFGAEYGYPIAIKAAYGGG
                     GRGMKVVENPESAAAAFESAQRESLAYFGRDECYMERYLTKPRHVEVQIIADEHGNCV
                     YLATRDCSAQRRHQKLIEEAPAAEIPADIIKQMGEAAVRVAKGCDYVNAGTVEFLYED
                     GGFYYLEMNTRLQVEHCVTELITGVDLVEMQLRVASGEELPFTQDDVQINGHAFEVRI
                     NCEDVAGGRFLPSPGTITKLRIPSGNGVRWDGGYEEGDEISQFYDNLAGKLCVWAKDR
                     PTAIARMIRALEEMVIEGVATTIPADLAILKHPDFAAFQHSTKWVETVLDLKDVSSKP
                     AKAPEPVDGEEPKVRRDVDVEVNGKKFSVSMWVPESAGAPAAGGGAAKPKPRRAAGAG
                     ASAAAASGSLTAPMQGTIVKVTVAEGDEVAEGDTICVLEAMKMENAITADKAGKVAKL
                     NVEAGQSVGSGDVLAVIE"
     gene            4840..5625
                     /locus_tag="PKY13_07845"
     CDS             4840..5625
                     /locus_tag="PKY13_07845"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT79883.1"
                     /translation="MDAPRATSALTGTRFSRFHWVDETGSTNADLLRVARDGGPEQVL
                     GADHQTAGRGRMGRVWEAPPGASLLCSVLVRPPAGSLDPHVVTTALGLAAAEAVEAVT
                     GVAVGIKWPNDLVAGTSAAPDDRKLAGVLAESTVVDGRVDAVVAGIGVNVNWPDGFPD
                     ELAATAVSLRQLVGADVDRTDLVVAMLRRLEVHLADPSRVRDAVAERSATIGRTVRVE
                     RGDGSVVGLATGLTADGRLVLDVDGTLHEISVGDVVHLRPTDG"
     gene            complement(5709..6551)
                     /locus_tag="PKY13_07850"
     CDS             complement(5709..6551)
                     /locus_tag="PKY13_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT79884.1"
                     /translation="MTTAKVWVGDRLFAAADATVRFDDHGVTVGDGVFETIKLSDGIP
                     FALTRHLRRLAGSAARMGIPMPGEDRLTEAVAAVCASHDGDGFLRVTLTAGPGPLGSN
                     RGDLDPTLVVAVRPGAVRTDPTDVLVVPWTRNERGPLAGVKSTSYGDNVVALVAAADV
                     GAGEALFANTRDELCEGTGSNVFVGFGKRLVTPPLDSGCLAGVTRELVLEAGIGEEAV
                     VPMSSLADASEMFLVSTGREVQPVRHVISPDGRRRELPWCSGPFTVEARRVWLEQIAP
                     QHDP"
     gene            complement(6548..7591)
                     /locus_tag="PKY13_07855"
     CDS             complement(6548..7591)
                     /locus_tag="PKY13_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOT79885.1"
                     /translation="MADEQGPTPVAVVGDRLCTDLVDVTSDLSALDSEGFWAVVIPYS
                     GAPTCARFATVRPAVPWSGRPWRGPAPDAWRSSLDEGAFSAGVRSIRESIAAGDVYQV
                     NLTRRLRARLDDPPNQRIEALGAALAVGNPAPFSAVVSLPDHGVAVASASPERFLSFD
                     GRSGDGDIVSSSPIKGTASTAEGFLPKDRAENVMIVDLVRNDLGRVCEFGSVAVPELL
                     AVEAHPGLFHLVSTVTGRLRPGCGWSDAIDATFPPGSVTGAPKIAACGVIDDLETEPR
                     GVYCGAIGWVDADRRVGDLNVAIRTFWIEEDELNFGTGGGITWDSTPEGEWAETELKA
                     RNLLRLASGPALA"
     gene            7712..9289
                     /locus_tag="PKY13_07860"
     CDS             7712..9289
                     /locus_tag="PKY13_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT79886.1"
                     /translation="MGIRRRRGHRSWPQRLLLLVNSILVIACIGAAGVFNFIRTKAES
                     LPVVDIGSEARQRPDLSGPRNFLLVGTDNSDSLAKGDPVKKGRNQGEHLADVIMILRV
                     DPATKEASLLSIPRDTWVPIAPSWSKSKINSAFGGNDGPSTLIATIKHNFGISIDNYV
                     QVDFGGFRDLVEVLGGLTVFNTHPIRDPATGLYLPLTGCIYVEPTQALAYARSRHLEY
                     QNTDVYAKGAKWIQDPTGDLGRITRQQDFLRQAAQRAIDEGIRNPSTAIGLVNAGIES
                     VQTDNDLNAGQIVDLISTFRDFSVENLKAEQIPTKGSGSKGVSYQEVIWGEAEGLLDI
                     YRGIRADGDVVPADVIVGLPPAVATSAELALQLDAAGFDAGVEDSSVVAGGKKSAKGT
                     VIRFGIRGIEAARVLASHIEGAVTYEFSATLPGRRLELIPGTVPPQLLDTPTPIDQVP
                     VPSIVEQKNGRGSTTTSSTSTTVVGRSSTTIPPVSTPTSVDSVTTTSTLNEDVTTTTA
                     IGVNTFDAKAATSCPGS"
     gene            9336..>10209
                     /locus_tag="PKY13_07865"
     CDS             9336..>10209
                     /locus_tag="PKY13_07865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOT79887.1"
                     /translation="MASKIAVIGTGYVGLTTGACLAHLGHDVVCADIDEAKVEALNNG
                     EIPILEDGLDNLVIEGRRAGRLRFVVGAANAVGDREFVYLCVPTPQGDDGSADLSYIE
                     SAAREIGPELAPEAIVINKSTVPVGSTRVVEQALQRGDVSVVSNPEFLREGSAVHDFL
                     NPDRVVIGAVDQAAAIRMAGLYIGLTAPIMVTDPASAETIKYASNAFLATKISFVNAV
                     AAVCEAVGADVNDVVLGMGYDRRIGQEFLKPGPGWGGSCFPKDTVALIHIAEEAGYDF
                     RLLKGVVDVNNEQFE"
BASE COUNT         1564 a         3512 c         3547 g         1586 t
ORIGIN      
        1 accagctggc ctggcaaccg ttcgtgaagc aggtcgagat gttgcccgag gcgttcggcg
       61 acgtggtcga cgccgatcag gtcgacgaga tcgaccgcgg ccggtacacc cgcaactccg
      121 atgccgacta cgggtacttc ggtgccgagc cgaggatcga cgtctggtgg ctctggatgg
      181 ggccgaccga cgcgagcggc tacacctggc tgttcgcgct cccgatcgtc gggctgttcg
      241 ccgctgcggg ctacctgctg gtgcgcgatc gccgcatcgg tcagtgggac gaggcgaccg
      301 cctcgagcgc cgaccggtac cagtcgacgt cagcagtcag ctcggacggc tcgcccacca
      361 gctcgatctg atccagcttg gtgtcgggca cgtacagggc ccggagccat tcgatcgctt
      421 cgtcgctgca cccgactcgc tgcatccccc gccagttgac cccccgcacg cgcccgggat
      481 tgcccgtgac ggtcgcgtag ggcggaacgg ccttcgtgac gatggtgccc atgccgagca
      541 tggcccccgg cccgacgacg agctcctggt gcaacaccga gttgagcccg aggttggcgt
      601 tcgctccgat acggccgtgg cctccgatga gtgccccgca cgacacggtc acgccgtccg
      661 acaggacacc gtcgtggggg acgtgcgcct tggtcatgac gtagcagtcg tcgccgatga
      721 tcgtctggcc gaacgcagga gcctgcacgg tggcgaactc gcggatgacg ttgcgatccc
      781 cgatgacgac gccgccgtga acggccggct cgtcccaggc ggtggggtgg tcgccaccgc
      841 gcatctcgcc cggagtgccg atcgcggtgt ggggcccgat ccagttgccg ttgccgatcc
      901 gggcggggcc gaggaggacg gcgtaggggc cgatgacgtt gtcgccgccg agctcgacgc
      961 ccgggccgat caccgccgtg ggatgaacac gattggtgcc gtgagcactg gtcatcgcgc
     1021 cgaccctagt ggccgagcca tggtcccccg gccactagcc tgccgccatg gcccccacgt
     1081 cgaatgccct gcgcttcgtg atcatcggcg gcggccccgc cggcacccag gccgcaacgg
     1141 tcgctgcccg gctcggcgcc gacgtcacgc tgatcgaacg cgacatcatc gggggtgcgg
     1201 cgcacctgtg ggactgcatc ccgtcgaagg cgatgatcgc cacgggcgcc gccttcgggg
     1261 aattcggcga ggccgcccag atgggcctgc acgcccagac ggcgcagatc gacctcgacg
     1321 cgctccgtgg ccgcatcacc ggcatcgtgg ggcggctcga gcagtccggt cgccgcctgc
     1381 tcgagagcca gggggtgcgc atcgtcatgg gcaccggtcg actggtcgac gagcacaccg
     1441 tcgaggtgac gacgcccgac ggtctcaccg aggaactccc cgccgaccgg atcctggtat
     1501 caaccggaag tcgcccccgc atccccgagt tcgcccatcc cgacggcgac cgggtcctca
     1561 cgacccgcga cgcgtacccg cccaaggaga tccccgagca cctcatcgtg atcggttcgg
     1621 gcgtcacggg cgtcgagttc gtgcacatgt tcctgtcgct cggctccaag gtgacgctgg
     1681 tcgtcagccg gaatcaggtg ctgccccaga aggaccccga ggtcgccgcc gtgttggagg
     1741 acgacttcgt gcgccgcggc gtcaagctgc tcaagggtgc gcgtgcgacc ggcgtcgagc
     1801 gcaccgatga cggggtcaag gtcatttgcg acgacgggcg ggtggcggtc ggctcgcacg
     1861 cgctgctcgc catcggttcg attccgaact ccgaggggct cgggtttgcc gaggtcggcg
     1921 tcgagatgga cggcgggtac gtcagcacca ccgaccacca cctgcggacg aacctgccga
     1981 acatctacgt cgccggtgac ctctcgggga agctgccact cagttcggtc gcgtcgatgc
     2041 agggccgcaa gatcgccgag cacgtcatgg gcatgcacat cgtcgcccac cgccatctcg
     2101 actacgagaa ggcggcgtcg gccatcttca ccgagccgga gatcgcggat gtcggcctcg
     2161 ccgaggccga cgcgttcgcc gagggccgca aggtccgcgt caccaaggtc ccgttcgcca
     2221 cgacggcgaa ggcgctcatc aacaacgact cccgcgggtt cgtgaagctc gtgtccgatc
     2281 ccgccaccgg cgtggtgctg ggcgggtcga tcgtgggtca gcacgcagca gagctcatca
     2341 gcgtcatcgc ggttgccgtg accgcgggcc tccgggtcaa cgacctcgca gagagcgtgc
     2401 tcgtccaccc ggcgctcgcc gaggcgctga tggaagcagc tgagtgagcg agggcccctg
     2461 gcagcaaccg ccgccgaccc catgggcgcc ggtcgacgag accgtcggcc gggccggcgt
     2521 cattccgcca ctgtggaagc gcgtcgtggc ccgcgtggtc gacgaactgc tcctggcgat
     2581 cccacgtctg gcgctcacgc tgccgttcgt gacgtgggcg gatccgcccg agctgaacct
     2641 tccccagtgg gcgctcgctc tgtcggtcgt catcccgctg ctgtacgact tcgccttcgt
     2701 ggcgatctgg cgcgccacac cggggaagct gctcttcggg atcgcagtgt gcgactcgcc
     2761 cgacggcggc cgtgcatcga tccagcaggc cgcgctccga gcgctcgtcc ccagcatcgg
     2821 cagcgcgttg ctgctggtga tgcccacgtc ggaggcgtcg tcgttcctcg cgctgatcac
     2881 gccggtggtc tacgcctcgg tcgcctggga cccgcggcgg cgcggcctcc acgacaaggc
     2941 ggccggcacc atcgtgctgt atcgatgacc tcggccacct cgctgaggct cgctcccgac
     3001 tccgtcgggg gcttctagcg cttcggtacc tcggtggccg aggccatcag gcgccgctac
     3061 tcgatgacgg cgaggacgtc gcccgagccg acggactgac cggcctcgac gttgagcttg
     3121 gccaccttgc ctgccttgtc ggcagtgatg gcgttctcca tcttcatggc ctcgagcacg
     3181 cagatcgtgt cgccctcggc gacctcgtca ccctcggcga cggtcacctt gacgatcgtg
     3241 ccctgcatcg gggcggtgag cgaacccgac gccgcggcgg ctgaagcacc agcgccggcc
     3301 gcacggcgcg gcttgggctt ggcggcaccg ccgcccgctg cgggggcgcc ggcggactcc
     3361 ggtacccaca tcgacacgga gaacttcttg ccgttgacct cgacgtcgac gtcgcggcgg
     3421 accttgggct cctcgccgtc gacgggctcg ggggccttgg ccggcttcga ggacacgtcc
     3481 ttcaggtcga gcacggtctc gacccacttg gtcgagtgct ggaatgccgc gaaatcgggg
     3541 tgcttgagga tggcgaggtc ggcggggatc gtggtcgcca cgccctcgat gaccatttcc
     3601 tcgagcgccc ggatcatgcg ggcgatggcg gtcgggcggt ccttggccca cacgcagagc
     3661 ttgccggcga ggttgtcgta gaactggctg atctcgtcgc cctcttcgta gccaccgtcc
     3721 cagcgaacac cgttgccgga ggggatgcgg agcttggtga tcgtgccggg ggaggggagg
     3781 aagcggccgc cggcgacgtc ctcgcagttg atgcggacct cgaaggcgtg gccgttgatc
     3841 tggacgtcgt cctgggtgaa ggggagttcc tcgcccgacg cgacgcgcag ctgcatctcg
     3901 acgaggtcga cgccggtgat gagctcggtc acgcagtgct cgacctgcag gcgggtgttc
     3961 atctcgaggt agtagaaacc accgtcctcg tagaggaact cgacggtgcc ggcgttgacg
     4021 tagtcgcacc ccttggcaac gcggaccgcg gcctcgccca tctgcttgat gatgtcggcg
     4081 gggatctcgg cggcgggagc ttcctcgatc agcttctggt ggcgacgctg ggcggagcag
     4141 tcacgggtgg cgaggtagac gcagttgccg tgctcgtcgg cgatgatctg gacctcgacg
     4201 tggcgcggct tggtcaggta gcgctccatg tagcactcgt cgcggccgaa ataggcgagc
     4261 gactcgcgct gggccgactc gaacgcggcg gcggccgact ccgggttctc gacgaccttc
     4321 atgccgcgac cgccgccgcc gtaggcggcc ttgatggcga tcgggtagcc gtactcggct
     4381 ccgaagtcga tgatctgctg cggatcggtg atgagttcgg tcgtgccggg cacgccgtac
     4441 acctcgaccc gctcggccgc gaggcgggcc gagatcttgt cgcccatcac gtcgatcgcg
     4501 tcggggcggg gaccgatgaa cttcacgccc atgtcggtga tcgccttggc gaagtcgctg
     4561 ttctccgaga agaagccgta gccggggtgc accgcctcgg ccccggaacg ctggatcaca
     4621 tcgatcagct tctcggtgtt gatgtagctc tcggcggcgg tctgcccgcc gagggcgtag
     4681 gcctcgtcgg ccaggcgcac gtgcagtgcg tcccgatcga gctcggagta gacggcgacg
     4741 gtggagatgc cgagttcctt gcaggcccgg atgacccgga cggcgatctc accacggttt
     4801 gcgatcaaca ctttggaaaa cacgccatat cttgggcgaa tggatgcccc gcgtgccaca
     4861 tcagcgttga ccggaacccg attctcccga tttcactggg tcgacgagac cggttcgacc
     4921 aacgccgacc tgctccgcgt ggcccgtgac ggcgggcccg agcaggtgct gggtgccgat
     4981 caccagaccg ccggccgagg tcggatgggc cgcgtctggg aggccccgcc cggcgcctcg
     5041 ttgttgtgct cggtgctcgt gcggcctccg gcgggctcgc tcgaccccca cgtcgtgacg
     5101 accgcgctcg gtctcgcggc cgccgaggcg gtcgaggcgg tgaccggggt cgcggttggg
     5161 atcaagtggc cgaacgatct ggtggcaggc acgagcgccg ctcccgacga ccggaagttg
     5221 gcgggggtgc tcgcggaatc gaccgtcgtc gacggccgcg tcgatgccgt ggttgccggc
     5281 atcggcgtga acgtgaattg gcccgacggg ttccccgacg agctggccgc caccgccgtg
     5341 tccctccggc agctcgtcgg cgccgatgtc gaccggaccg atctggtggt cgcgatgttg
     5401 cggcgcctcg aggtccacct cgcggatccg tcccgggtgc gcgacgcggt cgccgaacgg
     5461 tcggcgacga tcgggcggac ggtcagggtg gaacgaggcg atggttcggt cgtcggcctc
     5521 gccaccggcc tcaccgccga cggtcgcctg gtgctcgacg tcgacggcac gctccacgag
     5581 atctccgtcg gcgacgtcgt ccacctccgc ccgaccgacg gttgaccgcg cccggggttc
     5641 tgtagtgcga ggagcgacgc cagcgtcgct cctcgcagca caaaaccccc gaggggcggg
     5701 gtggggcgtc aggggtcgtg ctggggggcg atctgctcga gccagacgcg gcgggcctcg
     5761 acggtgaacg ggccgctgca ccaggggagt tcgcggcgcc gaccgtcggg tgagatcacg
     5821 tggcgcacgg gttgcacctc gcgacccgtc gacacgagga acatctcgga cgcgtcggcg
     5881 agcgacgaca tcggtaccac ggcctcctcg ccgatgcccg cctcgagcac gagctcccgt
     5941 gtgaccccgg ccagacaccc cgagtcgagc gggggcgtga cgagccgctt gccgaagccg
     6001 acgaacacgt tggagcccgt gccctcgcac agctcgtccc gggtgttcgc gaacagcgcc
     6061 tcgcccgccc cgacgtcggc cgcggcgacg agtgccacca cgttgtcgcc gtaggaggtc
     6121 gacttgaccc cggcgagtgg gccacgctcg ttgcgcgtcc acggaacgac caggacgtcg
     6181 gtcggatcgg tgcggacggc gcccggacgg acggcgacca ccagcgtcgg gtcgaggtcg
     6241 ccgcggttcg agccgagcgg gccggggccg gcggtcagcg tgacccgcag gaatccgtcg
     6301 ccgtcgtggg acgcgcacac ggctgcgacc gcctcggtca gccgatcctc gcccggcatc
     6361 gggataccca tccgtgcggc ggagcccgcc agccggcgga ggtgacgagt cagcgcgaag
     6421 gggatgccgt cggacagctt gatggtctcg aagacgccgt cgccgacggt cacgccgtgg
     6481 tcgtcgaacc ggacggtggc gtcggcggcg gcgaacagtc ggtcacccac ccagaccttg
     6541 gccgtggtca cgcgagggcc ggtcccgacg cgagccgcag gagattgcgg gccttcagtt
     6601 cggtctccgc ccactcgccc tcgggtgtcg aatcccaggt gatgcccccg ccggtgccga
     6661 agttcagttc gtcctcctcg atccagaacg tgcggatcgc gacgttgaga tcgccaacac
     6721 ggcgatcggc gtcgacccag ccgatggcgc cgcagtacac accccgcggc tcggtctcga
     6781 ggtcgtcgat cacgccgcag gcggcgatct tgggagctcc cgtcaccgaa ccgggtggga
     6841 acgtcgcgtc gatggcatcg gaccagccgc aacccggccg caggcgaccc gtgaccgtcg
     6901 acacgaggtg gaagagcccg gggtgcgcct cgaccgcgag gagctccggc actgcgaccg
     6961 agccgaattc gcagacacgg cccaggtcgt tgcggaccag gtcgacgatc atcacgttct
     7021 cggcgcggtc cttcggcagg aagccctcgg ccgtcgaggc ggtgcccttg atcggggagg
     7081 agctgacgat gtcgccgtca cccgaccggc cgtcgaagga gaggaagcgt tccggtgacg
     7141 ccgaggccac ggcaaccccg tggtcgggga gggacacaac ggcgctgaac ggcgccgggt
     7201 ttcccacggc gagcgcggcg ccgagcgcct cgatgcgctg gttcgggggg tcgtcgagcc
     7261 gggcccggag ccggcgggtg aggttgacct ggtagacgtc gccggcggcg atcgactccc
     7321 ggatgctgcg gaccccggcg gagaacgcac cctcgtcgag gctggatcgc cacgcgtcgg
     7381 gagcaggacc gcgccagggg cggccgctcc agggcacggc agggcggacg gtcgcgaacc
     7441 gggcgcaggt cggcgctccc gagtagggga tgacgaccgc ccagaacccc tccgaatcga
     7501 gagcggacag gtcgctcgtg acgtcgacga ggtcggtgca gagccgatcg cccaccaccg
     7561 cgaccggggt cggtccctgc tcgtcggcca cgatccagac actacaggcg gggtaccgga
     7621 gcggaagaag ctcccgaggg acgagggagc gagcctgtgc ggaggtggcc ccgcccagac
     7681 acgagttgtg tgggcactag cctcgtccct cgtgggaatc cgacgccgac gcggccaccg
     7741 ctcatggccc cagcgcctgt tgctgctcgt gaactcgatc ctcgtgatcg cctgcatcgg
     7801 tgctgcgggc gtgttcaact tcatccgcac caaggcggaa tcgttgccgg tcgtcgacat
     7861 cggctccgag gcccgccagc ggcccgacct gtccggcccg cgcaacttcc tcctggtcgg
     7921 caccgacaac tccgacagcc tcgccaaggg tgaccccgtc aagaaggggc gcaaccaggg
     7981 cgagcacctc gccgacgtga tcatgatcct gcgcgtcgac ccggcgacga aggaggcgtc
     8041 actgctgtcg atccctcgtg acacgtgggt gccgatcgct ccgagctggt cgaagagcaa
     8101 gatcaactcg gcgttcggcg gcaacgacgg tcccagcacg ctcatcgcca ccatcaagca
     8161 caacttcggc atctcgatcg acaactacgt gcaggtcgac ttcggcgggt tccgcgacct
     8221 cgtcgaggtc ctcggcgggc tcacggtctt caacacccat cccatccgcg acccggcgac
     8281 gggcctgtac ctcccgctga cgggctgcat ctacgtggag ccgacccagg ccctcgccta
     8341 cgccagatcg cgtcacctcg agtaccagaa caccgacgtg tacgcgaagg gcgcgaagtg
     8401 gatccaggac ccgaccgggg accttgggcg gatcacccgc cagcaggatt tcctgcgtca
     8461 ggccgcccag cgtgcgatcg acgaaggcat ccgcaacccg tcgaccgcga tcggtctggt
     8521 gaacgccggc atcgagtcgg tgcagaccga caacgacctg aacgccggtc agatcgtcga
     8581 cctcatcagc acgttccgtg acttctcggt cgagaacctg aaagccgagc agatccccac
     8641 caagggttcc ggcagcaagg gggtctcgta ccaggaggtg atctggggcg aggccgaggg
     8701 attgctcgac atctaccgcg gcatccgtgc cgacggcgac gtcgtaccgg ccgacgtgat
     8761 cgttggcctc ccacccgccg tcgccacctc cgccgaactc gcgctgcagc tcgacgccgc
     8821 cgggttcgac gccggcgtcg aggacagttc cgtggtcgcc ggcggaaaga agagcgccaa
     8881 gggcacggtc atccggttcg gcatccgagg aatcgaggca gcccgggtcc tcgccagcca
     8941 catcgaggga gccgtgacct acgagttcag cgcgacgctg cccggccggc gtctcgaact
     9001 gatcccgggg accgtgccgc cacagctgct cgacaccccg acgccgatcg accaggtccc
     9061 ggtgccgagc atcgtcgaac agaagaacgg tcgggggagc accacgacgt catcgacgag
     9121 caccacggtg gtcggccgga gttccaccac gattcctcca gtgtccaccc caacgagtgt
     9181 ggattcggtc acgaccacct ctaccctgaa cgaagatgtg accacgacga cagcgatcgg
     9241 cgtgaacacg ttcgacgcca aggccgcgac ctcgtgtccg gggtcgtgac accattcgga
     9301 agtcggtccg tcgggacctg agcaaagagg aactgatggc aagcaagatc gcggtgatcg
     9361 gtactggcta tgtcggattg acgacgggcg cttgcctggc ccatctcggc cacgacgtgg
     9421 tgtgcgcgga catcgacgag gccaaggtcg aggcgctcaa caacggcgag atcccgattc
     9481 tcgaggacgg gctcgacaac ctggtgatcg agggtcgtcg tgccggtcgc ctgcgcttcg
     9541 tcgtgggtgc cgccaatgcc gtcggtgacc gcgagttcgt gtatctgtgc gtgccgacgc
     9601 cccagggcga cgacgggtcg gccgacctct cctacatcga gtccgccgcc cgggagatcg
     9661 gtcccgaact cgcgcccgag gcgatcgtca tcaacaagtc cacggtgccc gtcggctcga
     9721 cccgtgtggt cgagcaggcc ttgcagcgcg gcgacgtgtc ggtcgtgtcc aaccccgagt
     9781 tccttcgtga gggttcggcg gtgcacgact tcctgaaccc cgaccgcgtc gtcatcggcg
     9841 ccgtcgacca ggcggccgcc attcgtatgg ccggtctcta catcggcctg accgcaccca
     9901 tcatggtcac cgaccccgct tcggccgaga ccatcaagta cgcctccaac gcgttcctcg
     9961 cgaccaagat cagcttcgtg aacgccgttg ccgccgtctg cgaggcggtc ggcgccgacg
    10021 tgaacgacgt ggtgctgggc atgggctacg accggcggat cggccaggag ttcctgaagc
    10081 ccggtccggg ttggggtggt tcctgcttcc ccaaggacac ggttgcgctg atccacatcg
    10141 ccgaggaggc cgggtacgac ttccggctgc tcaagggtgt ggtcgacgtc aacaacgagc
    10201 agttcgagc
//
LOCUS       DAOSOA010000095        10145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6621_length_10145_cov_8.978196, whole genome shotgun sequence.
ACCESSION   DAOSOA010000095 DAOSOA010000000
VERSION     DAOSOA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6621_length_10145_cov_8.978196"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1851
                     /locus_tag="PKY13_07870"
     CDS             124..1851
                     /locus_tag="PKY13_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOT79888.1"
                     /translation="MTNATATPTPRVGRSSEHGYVLMTCALVLVPLLIVAGMAIDFGG
                     NYWQGVKMQRATDAAALAGVVWLPDLTKATQVANDAMTKNGWANGGGNTVTITQVGAR
                     RLKVSLTAPATGYLAAKLLKTNYNMTRSATAEYLQAIPMGSPIASLANDPESAAAPPQ
                     IWLNQSGSGSTKNNGDEFTSGVCGTSGSGFSSGCTGSVNNEISDDGYIYVTRVSALGS
                     GPLKMQAFDPAFIYTDDNCGTNSYSGGEETTLIAQYSGGSVADAKAAQRYDYSNKAYC
                     PGDQRINSLSNVTTTFIVRAPDTTPTDMTDNPAICAISFSPYTSGYFSKLDQTRPGWN
                     SNQGLENLPFWKIFRRWVDICTISSPVVGDYVTQITTTADQSSPQFSTTASLSPSLGA
                     GSLEQRDPGIVTGGHNRFALRSGFGASVNGTGVSVFAGGRLPIFVNQNTSSATFYLAK
                     IQPQYAGATLSLQFFDIADTAGSATMTVQPPTDSNYSTFPSCAFVKDASPPTAISSSG
                     CSVSGLTSAQYDARIITLTLVVPTDYTCNTSVATGCWLKVKLDFTGAPADTTTWSASL
                     LGDPVRLVE"
     gene            complement(1873..3018)
                     /locus_tag="PKY13_07875"
     CDS             complement(1873..3018)
                     /locus_tag="PKY13_07875"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT79889.1"
                     /translation="MTSPEVHGVCDERFAAVRQVFEGNFANGLEAGASVAVTVDGEFV
                     VDLWGGVADVDTGRPWERDTIVNVWSSTKTMVALVALELADRGVLDLHAPVATYWPEF
                     AAAGKEGIEVRHVLGHTAGLSGWRDPMTVDGLYDLPTANSLLAAQAPLWEPGTASGYH
                     AITEGHLMGEIVRRVTGVSFGEYFRTTFAEPLGADFQIGVGATDDARVATIVPPSFPL
                     GGGVDLEVGSIPHMTLCNPVVHATISATEAFRRAELPAANGHGNARSMARVQSIVSNG
                     GVLDGHRFLSEAGIEALFEEQACGVDLVQAVPLRLGVGYGINGPELPVSPNPRACYWG
                     GWGGSIVLNDLDANVTFAYAMNRMGEGSTVDFRGVGLALAMHTVLMA"
     gene            complement(3015..4106)
                     /locus_tag="PKY13_07880"
     CDS             complement(3015..4106)
                     /locus_tag="PKY13_07880"
                     /inference="COORDINATES: protein motif:HMM:NF022447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HOT79890.1"
                     /translation="MIPPAQDYASSRASFLDLVRAAGSVVESHLHPEQGPAGEELACD
                     VARFGAPPGDAATVVLISSGLHGVEGHAGWGLQRLLVESGRLATLTPSVAVVLVHAVN
                     PFGFAWSRRVDHDNIDVNRNFVDVDHLPANPRYAEVDSALNPTADQFDPDDLSFLAEL
                     QELVDLAGMLEAFRVISGGQYSHPQGVQFGGQHESWSRRTLERIWADHLPGAVRAVAL
                     DIHTGLGPTGRLTVFQTANEDEPAAELGRSWFPDYLYRADRDEEQATDTGLMGPGFDS
                     WVAASEDLVADTAAFVIEFGTHDPIQGVTVFRADNWLHHHGDPRSDLGVEIRRRMRDF
                     FFVEDEEWRRVVAEQGMAAITAALDGVGA"
     gene            complement(4103..5740)
                     /locus_tag="PKY13_07885"
     CDS             complement(4103..5740)
                     /locus_tag="PKY13_07885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT79891.1"
                     /translation="MDLAARHYRDAVVVTSGGNGGPVPTSVATLGDRIVAVGSDADCV
                     AALPTDHEIVDVGGRTLSPGLVDAHLHPLIMAVFEQQLLLDQCGCLADVFDLVAERAL
                     QGDDGTAIMGFQLDDAVLAERRLPTAIELDACGRGRPVILVRRDGHHAVASTSALVAA
                     GFDRPGNEPAGGVVERDASGRPTGLVRENAVAPLLAFLPDLDLDGLALGVDAWADRLV
                     RQGVTGISAICQTTAEGPSGVAGELEVYGWEMFVDRVPFDVQTILVAPDLAAVDAARS
                     TSLHAPARRRRVDAVKLFIDGTLGGHTACMHQPFSDRPETAGMRTMDSDEAERRTREA
                     HARGLAVCVHAIGDRANREAAEMFRRVLADDPGPHRHRVEHASVLDDPTVELLAELGV
                     SAVVQPISLRSERHWLRRRVGDERMMRTYPFRSLLDAGVNVAGSSDAPIERTDPFAAM
                     AAATERVAGHEPQAISATEALGLYTTGAARARGCETDQGAIRVGGLADLVVLSADPRT
                     SSPDQLERIDVLSTVIGGAECYRDPRLSSRPSREGAP"
     gene            complement(5744..6937)
                     /locus_tag="PKY13_07890"
     CDS             complement(5744..6937)
                     /locus_tag="PKY13_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOT79892.1"
                     /translation="MDQTIRAGSGERVERIELFELRVPLSDLARGAMAESPNGLGMAI
                     PSEAPWLEADFLYCRLVDGDGNEGWGEAYVWLPETGVSQRELALSIEHHLGAYVLGAR
                     PADTQALAARFDRNVARNEIAKGLVDLACHDLAARQVGRPVHDLIGGAAVDRIPLCGL
                     VPLGSPDTVAALCAGYQRGGYGTVRIKLGTGPTADRDVIAAVRERCGDDLRVRVDYNQ
                     AYSAAGAIRALRLLEPFGIDAAEQPLPLGDLLGMVEVQRQTSIPLFLHEGFFSMSDLV
                     TLIELGGCRVVGVNLERPGGLTGALRAIDYGAARGLRTIIHNQSLGLGTAAQLHLAAA
                     RFDRLGHAVELAGDVMFDEHLVVDRCVQADGHLLVPTGPGWGVTVDRDAIERHLVAEP
                     IVVRG"
     gene            complement(6986..7831)
                     /locus_tag="PKY13_07895"
     CDS             complement(6986..7831)
                     /locus_tag="PKY13_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOT79893.1"
                     /translation="MVGRSIQAKVAELADSLPDSQRRVAALVVSDPQSVAFGTLASVA
                     EQAATSTPTVIRFAAQLGYDGFTALRDAVRSEVSGQLRSAASRLRRSSDEPLIEEALR
                     VERENVERTFAQLDPTDLDRAVELVSDTSRRLWVLANSQLAGLGGHLADELQLCRDRV
                     VRLEGSEFRIATTLGKLRAGDVLVSIDTQRHEGWFVRVQRQAVGRGAVPVALTDRLPC
                     SLDLTGGVAMTFACETTSPFESQVGVLALGNLLVAAVVDRLRSDVTERVDVLERLWVD
                     DGLFD"
     gene            complement(7923..9167)
                     /locus_tag="PKY13_07900"
     CDS             complement(7923..9167)
                     /locus_tag="PKY13_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT79894.1"
                     /translation="MSAEAASLTTAEADGLLLAVVLDDETRQDPVPVYRRLLAESPRW
                     ESATGMTILAGYHDGLEYLRNPHWGRAEVDMDLPPTLNGSARRSDRDTTTMLFLNPPD
                     HTRIRSLVARAFTPRRVEELRGRIDELLTPILDEVADAGECDIMSTLAVPFPVAVISA
                     LLGVPQDPTGNFTSLVRDMTSFIDAAADETAITRAEAAAVQMAGYFLEMIEEKRATPD
                     DGLLSALIQVEAEGERLSEDELLANTLLMYAAGFETTSNLIGNGLRALLAHPDELARL
                     RSDRSLMSSAVQEILRFDSPVQLNVRFALQDDQLFGAPVPRGTAVMILQGSGNHDPAA
                     YDRPERFDVGRFAGEGVPAPLSFGWGAHHCLGAHLARAEGEIVLGALLDRFEHIEVVG
                     TEPRYRPSFTLRGLDQMTLAVS"
     gene            complement(9164..9814)
                     /locus_tag="PKY13_07905"
     CDS             complement(9164..9814)
                     /locus_tag="PKY13_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOT79895.1"
                     /translation="MPRLREIPRDEVTAPVVTMMYDFLFGERDPVAEPGTTTGTPGDW
                     WTVFANSPDIFEHAVQGFGIYRSSRRQLDPVLRELGQTRAGWAVGSQFVFSQHCKSCR
                     LLGMSESKIQAIAYWQASDEFTPVERLVLAYTDALAVSGGRMHDQLFAALREHLSDLE
                     ILELTYITSLYVMHAIMSKALRTEWDNRDDPIVELEGPEGFQGYDVGAAISGGAAE"
     gene            9866..>10145
                     /locus_tag="PKY13_07910"
     CDS             9866..>10145
                     /locus_tag="PKY13_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOT79896.1"
                     /translation="MSAVAVGRFAPSPTGDLHLGNLRTAVLAWLWSRHAGSEFVVRME
                     DLDRVTSSPEHEASQLRDLAAIGLDWDGPVVRQSDRFDLYHDAIATLDR"
BASE COUNT         1692 a         3690 c         3308 g         1455 t
ORIGIN      
        1 caacgggccc gactacctcg gtgtcgccat ctccctgacc gtgcccacct tcacgcggct
       61 cgtcggcacg accaagacct tcaacgaccg cttcaccatg cgtctcgacc cgtccggact
      121 gggatgacga acgcaaccgc cacccccacg ccccgcgtcg gtcgctcgtc ggagcacggc
      181 tacgtgctca tgacctgcgc cctggtgctg gtgccactgc tcatcgtcgc cggcatggcc
      241 atcgacttcg gaggcaacta ctggcagggc gtcaagatgc aacgcgccac cgacgccgcc
      301 gcgctggccg gggtcgtctg gctccccgac ctcaccaagg cgacccaggt cgcgaacgac
      361 gccatgacca agaacggctg ggcgaacggc ggcggcaaca ccgtcaccat cacccaggtc
      421 ggtgctcgcc gactgaaggt ttccctcacc gccccggcga ccggctacct cgccgccaag
      481 ctcctcaaga cgaactacaa catgacccgc tcggccaccg ccgagtacct ccaggcgatt
      541 cccatgggca gccccatcgc ctccttggcg aacgaccccg agtcggccgc cgcaccaccg
      601 cagatctggc tcaaccagtc cggatcgggt tcgaccaaga acaacggcga cgagttcacc
      661 tcaggcgtct gcgggacctc cggcagcggg ttcagctccg ggtgcaccgg gtcggtgaac
      721 aacgagatca gcgatgacgg gtacatctac gtcacccggg tctcggccct cggaagcgga
      781 ccgctcaaga tgcaggcgtt cgacccggcg ttcatctaca ccgacgacaa ctgcggtacg
      841 aactcctact cgggaggcga ggagaccacc ctcatcgccc agtactccgg tggatcggtc
      901 gccgacgcca aggccgcgca gcgctacgac tactccaaca aggcgtactg ccccggcgac
      961 cagcgcatca acagtctcag caacgtcacg accaccttca tcgtccgcgc ccccgacacc
     1021 accccgaccg acatgacgga caaccccgcg atctgcgcca tcagcttctc gccttacacc
     1081 tccgggtact tctcgaaact cgaccagacc cgaccggggt ggaactcgaa ccaggggctc
     1141 gagaacctgc ccttctggaa gatcttccga cgttgggtcg acatctgcac gatctcgtcg
     1201 ccggtcgtcg gcgactacgt cacccagatc accacgacgg ccgaccagtc gagtccgcag
     1261 ttctccacga ccgcctcgct gagcccatcg ctcggtgccg gcagtctcga gcaacgcgac
     1321 ccgggcatcg tcaccggcgg acacaaccgg ttcgcgctgc gctcggggtt cggggcgagt
     1381 gtcaacggca ccggcgtgtc ggtgttcgcc ggtggccggc tgcccatctt cgtcaaccag
     1441 aacacgtcgt cggcgacgtt ctacctggcg aagatccaac cgcagtacgc cggcgcgacg
     1501 ctctcgctcc agttcttcga catcgccgac accgccggtt cggcgacgat gacggtgcag
     1561 ccaccgaccg acagcaacta ctcgacgttc ccctcgtgtg ccttcgtgaa ggacgcctcg
     1621 ccgccgaccg caatctcgtc gtcgggctgc tcggtctcgg gcctcaccag cgcccagtac
     1681 gacgcccgga tcatcacgct cacgctggtc gtcccgaccg actacacgtg caacacgtcg
     1741 gtcgccacgg gttgctggct caaggtgaag ctcgacttca ccggcgctcc cgccgacacg
     1801 accacctggt cggcatcgct cctcggtgat cccgtccggc tggtcgagtg accctgccga
     1861 cagcggaacc gatcacgcca tcagcaccgt gtgcatcgcc aacgcgaggc ccacgccgcg
     1921 gaagtcgacc gtgctcccct cgcccatccg gttcatcgcg tacgcgaacg tgacgttcgc
     1981 atcgaggtcg ttcaacacga tcgagccgcc ccagccgccc cagtagcagg cgcgcgggtt
     2041 ggggctgacc ggcagctcgg gcccgttgat gccgtagccg acaccgaggc ggagcggcac
     2101 cgcctgcacg aggtcgacac cacaggcctg ctcctcgaac agcgcctcga tcccggcctc
     2161 cgacaggaac cggtggccgt cgagcacccc gccgttcgac acgatgctct ggaccctcgc
     2221 catcgaccgg gcgttgccgt gaccgttggc agccggcagt tcggccctcc ggaacgcctc
     2281 ggtcgcggag atcgtggcgt gcacgaccgg gttgcacagg gtcatgtgcg ggatcgatcc
     2341 cacctcgagg tcgactcccc cgcccaacgg gaacgagggc ggaacgatcg tggcgaccct
     2401 cgcgtcgtcg gtggcgccga ccccgatctg gaagtcggcg ccgaggggct cggcgaacgt
     2461 cgtgcggaaa tactcgccga acgacacacc ggtgacgcgg cgaacgatct cgcccatcag
     2521 gtggccctcg gtgatggcgt ggtaccccga cgccgtgccc ggctcccaga ggggcgcctg
     2581 cgctgcgagg agcgagttgg cagtgggaag gtcgtagagc ccgtcgaccg tcatcggatc
     2641 ccgccacccc gacaggcccg ccgtgtgtcc gagcacgtgg cggacttcga tgccctcctt
     2701 cccggcggcc gcgaactccg gccagtaggt cgccaccggc gcgtgcaggt cgagcacacc
     2761 ccgatcggcg agttccagcg cgacgagcgc gaccatcgtc ttggtcgacg accagacgtt
     2821 cacgatcgtg tcgcgctccc agggtcgccc ggtgtcgaca tcggcgacgc caccccacag
     2881 gtcgacgacg aactcgccgt cgaccgtgac ggccacgctc gctccggcct cgagtccgtt
     2941 ggcgaagttc ccctcgaaca cctggcggac cgcggcgaac cgctcgtcgc acaccccgtg
     3001 gacctcgggc gaggtcacgc gccgaccccg tcgagcgcgg cggtgatcgc cgccatgccc
     3061 tgctcggcga cgacccgccg ccactcctcg tcctcgacga agaagaagtc gcgcatccgc
     3121 cgccggatct ccacgccgag atccgaccgg gggtcgccgt ggtggtggag ccagttgtcc
     3181 gcgcggaaga ccgtcacgcc ctggatcggg tcgtgggtgc cgaactcgat cacgaaggcg
     3241 gcggtgtcgg cgaccaggtc ctcgctcgcc gcgacccacg agtcgaagcc gggtcccatg
     3301 aggccggtat cggtcgcttg ctcctcgtcg cggtcggccc gatagaggta gtccgggaac
     3361 caactgcgac cgagttctgc ggccggctcg tcctcgttcg ccgtctggaa cacggtgagc
     3421 cgaccggttg gcccgaggcc ggtgtggatg tcgagggcga ccgcccgcac ggcgccggga
     3481 agatggtcgg cccagatccg ttcgagcgtg cgtcgcgacc agctctcgtg ctgcccaccg
     3541 aactgcaccc cctgcggatg gctgtactgg ccgccgctga tgacccggaa cgcctcgagc
     3601 atcccggcga gatccaccag ttcctgcaac tccgccagga acgagagatc gtcgggatcg
     3661 aactggtcgg cggtcggatt gagcgccgag tccacctcgg cgtaccgggg attggccggg
     3721 aggtggtcca cgtcgacgaa gttgcgattc acgtcgatgt tgtcgtggtc gacccgtcgt
     3781 gaccacgcga agccgaacgg gttgaccgcg tggacgagca ccaccgcgac cgacggcgtc
     3841 aacgtggcga gacgcccgga ctccacgagc agtcgctgca ggccccaccc cgcgtgcccc
     3901 tcgaccccgt ggagccccga ggagatgagg accaccgtcg cagcgtcgcc cggcggcgcg
     3961 ccgaagcggg cgacatcgca cgccaactcc tctcccgccg gaccttgttc gggatggagg
     4021 tgcgactcga cgaccgagcc ggcggcgcgt accaggtcga ggaacgacgc tcgggaggac
     4081 gcatagtcct gggcgggagg aatcatggcg ctccttcacg ggagggacgg gaggacaggc
     4141 ggggatcgcg atagcactcg gccccgccga tgacggtcga caggacgtcg atgcgttcga
     4201 gctggtcggg cgacgacgtc ctcgggtcgg cggacaacac gaccagatcg gccaggccgc
     4261 cgacgcgaat cgcaccctgg tccgtctcgc acccccgcgc ccgagccgca ccggtcgtgt
     4321 acaaaccgag cgcctcggtc gcgctgatcg cctgcggctc gtgaccggcc acccgctcgg
     4381 tcgcggccgc catggccgca aacggatcgg tgcgttcgat cggggcgtcc gacgacccgg
     4441 cgacattcac cccggcgtcg agcaacgacc ggaagggata cgtgcgcatc atccgttcgt
     4501 cgcccacacg gcgccgtaac cagtgacgct cgctgcgcaa gctgatcggc tgcacaacgg
     4561 cggacacgcc gagttcggcc agcagctcga ccgtcgggtc gtcgagcacg gaagcgtgct
     4621 cgacgcgatg ccggtgcggt cccggatcgt cggcgagcac ccgccggaac atctccgccg
     4681 cttcgcggtt cgcgcggtcg ccgatggcgt ggacgcacac ggccaggccg cgggcatggg
     4741 cttcccgggt gcgccgctcg gcttcgtcgg aatccatcgt tcgcatacca gcggtctccg
     4801 gccgatcgga gaagggctga tgcatgcagg cggtgtggcc gccgagcgtt ccgtcgatga
     4861 acagcttcac ggcatccacc cgccggcggc gggccggagc gtgaagcgag gtcgatcggg
     4921 ccgcgtcgac ggcagcgaga tcgggcgcga ccaggatcgt ctgcacgtcg aacggcaccc
     4981 ggtcgacgaa catctcccag ccgtacacct cgagttcgcc ggccactccc gaaggtccct
     5041 cggcggtggt ctgacagatc gccgagatcc cggtgacgcc ctggcgcacc agccgatccg
     5101 cccacgcgtc cacgccgagc gccaacccgt cgaggtcgag gtcgggcaga aacgcgagga
     5161 gcggtgccac ggcgttctcc cgcacgagcc cggtcgggcg gcccgatgcg tcgcgttcca
     5221 ccacgccacc ggccggctcg ttcccggggc gatcgaaccc tgctgcaacc agcgcgctgg
     5281 tggacgcgac cgcatggtgc ccgtcgcgac gcacgaggat cacgggccgc ccgcgcccac
     5341 acgcgtcgag ctcgatcgcg gtcgggagac gccgctcagc gaggaccgcg tcgtcgagct
     5401 ggaaccccat gatcgccgtg ccgtcgtcgc cctgcagcgc tcgttccgcc acgagatcga
     5461 acacgtcggc aagacaaccg cactggtcca acagcagctg ctgctcgaac accgccatga
     5521 tcaacgggtg gagatgggcg tcaacgaggc ccggcgacag ggtccgcccg cccacgtcga
     5581 cgatctcgtg atcggtcggc aacgcggcga cacagtccgc gtcggagccg accgcaacga
     5641 tccggtcgcc caacgtcgcc acgctcgtcg gcaccggccc gccgttgccg ccagacgtca
     5701 ccacgaccgc gtcgcgatag tggcgggcag cgaggtccac gactcagccc cgaacgacga
     5761 tcggctcggc gacgaggtgc cgctcgatgg cgtcacgatc gaccgtcaca ccccacccgg
     5821 gcccggtcgg gaccaacagg tgcccgtccg cctggacgca ccggtcgacc acgaggtgct
     5881 cgtcgaacat cacgtcgccg gccagttcca ccgcatgccc gagccggtcg aaccgggcgg
     5941 cggcgaggtg caactgcgct gcggtgccga ggccgaggga ctggttgtgg atgatggtgc
     6001 gcagtccccg tgccgccccg tagtcgatcg cccggagcgc cccggtgagg ccgccgggtc
     6061 gctcgaggtt caccccgacc acccgacacc ccccgagctc gatcagcgtg accaggtcac
     6121 tcatcgagaa gaacccttcg tggaggaaca acgggatcga cgtctgtcgc tggacctcga
     6181 ccatgccgag gaggtcaccc agcggcaacg gttgctcagc agcgtcgatg ccgaagggct
     6241 cgagcagtcg gagcgcgcgg atggctcccg ccgccgagta cgcctggttg tagtcgaccc
     6301 ggacacggag gtcgtcgcca cagcgttcac gcacggcggc gatcacgtcg cgatcagccg
     6361 tcggccccgt cccgagcttg atccgcaccg tgccgtagcc accgcgttgg tatccggcgc
     6421 agagcgccgc gaccgtgtcc ggcgaaccca gcgggacgag cccacacagt gggatccgat
     6481 cgacggcggc accgccgatg aggtcgtgga ccggccgccc gacctgccga gcagcgaggt
     6541 cgtggcacgc gaggtcgacg aggcccttgg cgatctcgtt gcgcgccacg ttgcgatcga
     6601 atcgggcggc gagggcctgg gtatcggcgg gtcgggcgcc cagtacgtag gcgcccaggt
     6661 ggtgctcgat cgacagcgcg agttcgcgtt gcgacacccc cgtctcgggc aaccacacgt
     6721 acgcctcgcc ccagccctcg ttgccgtcgc cgtcgaccag ccggcagtag aggaagtcgg
     6781 cctcgagcca gggcgcctcg ctcgggatgg ccatgccgag cccgttggga ctctccgcca
     6841 tcgcgccccg cgccaggtcg gagagtggga cacgcagctc gaacagctcg atgcgttcga
     6901 cccgttcccc tgaacccgcc cgtatcgttt gatccacgat gcgtaccgta tcgtacaagt
     6961 ggtgatggtg ctggttcgga tcagttcagt cgaacaggcc gtcgtccacc cacaggcgct
     7021 cgagcacatc cacccgctcg gtcacatcgg accggagccg atcgaccacg gccgcgacga
     7081 gcaggttgcc gagagcgagg acccccacct gactctcgaa cggactcgtc gtctcgcacg
     7141 cgaacgtcat ggcgacaccg ccggtgaggt cgagcgagca cgggagccga tcggtgaggg
     7201 cgaccggcac ggcgccacga cccaccgctt gccgctggac ccggacgaac cagccctcgt
     7261 gccgctgggt gtcgatcgac acgagcacgt cgccggcccg gagcttgccc agcgtcgtcg
     7321 cgattcggaa ctccgaaccc tccagacgca cgacgcggtc gcgacagagc tgcagctcgt
     7381 ccgcgagatg cccacccagg ccggccagct gcgagttcgc aaggacccac aggcggcgcg
     7441 acgtgtccga gaccagctcg acggctcggt cgagatcggt cgggtcgagc tgggcgaacg
     7501 tgcgctcgac gttctcgcgc tcgacacgca gcgcctcctc gatcagtggc tcgtcgctcg
     7561 accgacgaag ccgcgaggcc gccgaacgca actgacccga cacctcggac cgcaccgcat
     7621 cgcggagcgc cgtgaacccg tcgtagccga gttgcgccgc gaacctgatc accgtcggcg
     7681 tgctcgtcgc cgcctgctcc gcgaccgagg ccagcgtccc gaacgcgacg ctctgcggat
     7741 cggacaccac gagggcagcg acccgccgct gcgagtccgg caacgaatcg gcgagctccg
     7801 ccaccttcgc ctggatcgaa cggcccacca tgggcgacac cgtaaccggc tcgctccgcg
     7861 tcagatcgat gcgccgaaca ccggcgcgac gacgaccgca atcgccgcga tcaacccgtg
     7921 tctcaggaga cggccaacgt catctggtcg aggccgcgca aggtgaagct cggccgataa
     7981 cgcggctcgg tgcccacgac ctcgatgtgt tcgaagcggt cgagcaacgc gccgagcacg
     8041 atctcgccct cggctcgcgc caggtgggcc ccgaggcagt ggtgagcacc ccagccgaag
     8101 ctcaacggcg ccggaacgcc ctcgccggcg aatcgcccca cgtcgaaccg ttccggacgg
     8161 tcgtacgccg ccgggtcgtg attgcccgat ccctgcagga tcatgaccgc ggtaccgcgc
     8221 ggcaccgggg ctccgaagag ctggtcgtcc tgcagcgcga acctcacgtt caactgcacc
     8281 gggctgtcga agcgcaggat ctcctgcacg gccgaactca tgagcgaccg atcggaccgc
     8341 aggcgcgcca gctcatcggg atgagcgagc aacgcccgca gaccgttgcc gatgaggttc
     8401 gacgtcgttt cgaacccggc ggcgtacatg aggagcgtgt tggccaacag ttcgtcctcc
     8461 gacaggcgtt cgccctcggc ctcgacctgg atcagcgccg acaggagacc gtcgtcgggt
     8521 gtcgcccgct tctcctcgat catctcgagg aagtagcccg ccatctgcac cgccgccgct
     8581 tcggcgcgag tgatcgcggt ctcgtccgcg gcggcgtcga tgaacgaggt catgtcccgg
     8641 accaggctcg tgaagttccc ggtcggatcc tgaggaacgc ccagcaacgc ggagatcacc
     8701 gcgacgggga acgggaccgc gagcgtcgac atgatgtcgc actccccggc gtcggcgacc
     8761 tcgtcgagga tcggggtcag gagttcgtcg atccggccgc ggagctcctc gacgcgtcgc
     8821 ggcgtgaacg cccgcgccac caatgaacgg atccgcgtgt ggtcgggcgg attgaggaac
     8881 agcatcgtgg tcgtgtcgcg atcggaccga cgggccgaac cattgagcgt gggcggcagg
     8941 tccatgtcga cctcggcgcg accccagtgc ggattccgca ggtactcgag accgtcgtgg
     9001 tagccggcga ggatcgtcat gccggtggcg ctctcccaac gcggcgattc cgcgagcagt
     9061 cgccggtaga ccgggacggg gtcctggcga gtctcgtcgt cgagcacgac ggccagcagc
     9121 agaccgtcgg cctcggcagt ggtgagcgac gccgcctcgg cgctcactcc gccgctcccc
     9181 cgctgatggc cgcgccgacg tcgtacccct ggaacccctc gggaccttcg agctcgacga
     9241 tcgggtcatc gcggttgtcc cactcggtcc ggagcgcctt gctcatgatt gcgtgcatga
     9301 cgtagagcga ggtgatgtac gtgagctcga ggatctccag atcgctcagg tgctcacgga
     9361 gcgcagcaaa caattggtcg tgcattcgac cacccgatac cgcgagcgca tcggtgtacg
     9421 cgagcaccag acgttcgacc ggagtgaact cgtcggacgc ctgccagtag gcgatcgcct
     9481 ggatcttcga ctcgctcata ccgagcaacc ggcacgactt gcagtgctgg gagaagacga
     9541 actggctgcc gaccgcccac cccgcccgag tctgcccgag ctcgcggagc accgggtcga
     9601 gctggcgacg cgagctgcgg tagatgccga agccctgcac cgcgtgctcg aagatgtcgg
     9661 gggagttggc gaagacggtc caccagtcac cgggagtgcc ggtggtcgta cccggctcgg
     9721 ccaccgggtc gcgctcgccg aacaggaagt cgtacatcat cgtgacgacc ggggccgtca
     9781 cttcgtcacg tgggatctcg cggaggcggg gcatctccgt accctaggac tccgtacccc
     9841 gatcgtctcg gagaacgctg gcatcatgtc ggccgtggcc gtcggacgat tcgcacccag
     9901 ccccacgggc gacctccacc tcggcaacct ccgcaccgca gtgctcgcct ggctgtggtc
     9961 acggcacgcc ggtagcgagt tcgtcgtccg gatggaggac ctcgatcggg tcacgtccag
    10021 tccagagcac gaggcgtccc agttgcgtga ccttgccgca atcgggctcg actgggacgg
    10081 ccccgtcgtg cgccagtccg accggttcga cctctaccac gacgccatcg cgaccctcga
    10141 tcgag
//
LOCUS       DAOSOA010000096        10124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6640_length_10124_cov_6.142715, whole genome shotgun sequence.
ACCESSION   DAOSOA010000096 DAOSOA010000000
VERSION     DAOSOA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6640_length_10124_cov_6.142715"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKY13_07915"
     CDS             <1..781
                     /locus_tag="PKY13_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT79897.1"
                     /translation="VVDARGVTVRYGKVVAVREVDLRLAEGEIVALMGRNGSGKSSLM
                     WALQGSGPRAGGIVSVTGSDGVAVDPGALKAGEARRRVGLVPQTPADLLYLATVAAEC
                     ARADADAAQPAGSCAALLGRLVGEIDDTIHPRDLSEGQRLALVLAIQLTAAPRAMLLD
                     EPTRGLDYDAKAGLGAVLRELADEGRSIMVSTHDVEFVASVADRVVVMAQGEVVADGT
                     TAEVVVASPTFAPQVAKVLAPEPWLTVEAVAAALARTEAPT"
     gene            778..1659
                     /locus_tag="PKY13_07920"
     CDS             778..1659
                     /locus_tag="PKY13_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT79898.1"
                     /translation="MTTQTIPDTIPDRRHDVMRLRARAVMVLSLASVAGLCMFLWPLL
                     VVPPTEFSHNTDAPFVFVLIMPVVVAVVLSELSSGGMDTKSLAMLGVLSAIGAALRPM
                     GAGVAGIETVFFMLVLAGRVYGPGFGFVLGNTTLFASALLTGGVGPWLPFQMLASAWV
                     GLGAGLLPWPRIRGRAEIALLAAYGAVAAYAFGFLMNMWFWPYSIGEGTELSFVPGDP
                     IWSNLHRFALFTLASSTWGWDTGRAITNVVAVALLGSTVLATLRRATRKANFGAPVTF
                     TAAHATADDGGSSPNRG"
     gene            complement(1768..2148)
                     /locus_tag="PKY13_07925"
     CDS             complement(1768..2148)
                     /locus_tag="PKY13_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT79899.1"
                     /translation="MTIELVYRAQTPSDARHFVRDALMNAPTVAVDTAELLTSELVTN
                     AITHACLSSALITLASDGDAVRVEVHDTSTDLPRLGSSLDPLDPGGYGLAIVDQMSTK
                     WGVEERTDGKVVWFELVLADDAPT"
     gene            complement(2255..2452)
                     /locus_tag="PKY13_07930"
     CDS             complement(2255..2452)
                     /locus_tag="PKY13_07930"
                     /inference="COORDINATES: protein motif:HMM:NF037605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HOT79900.1"
                     /translation="MIDPQAVAAHDRAVAAGAPTYVDPATGYAVMTSVYLLDRGYCCG
                     NGCRHCPYPPDEQRAAGRPGV"
     gene            complement(2449..2967)
                     /locus_tag="PKY13_07935"
     CDS             complement(2449..2967)
                     /locus_tag="PKY13_07935"
                     /inference="COORDINATES: protein motif:HMM:NF037679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT79901.1"
                     /translation="MARPSSRERILDTFEQVLLDDGAGSATLDAVAERAGISKGGLIY
                     HFPSKAALLAGLVERLMTRVDEIVAAAPDEPAELIRWYLSYDPSDPVELAVWRSLLAA
                     IHAVDDGLAQAISDAFVRFTRPLAVLDPFLAEQVRLIGDGLYLNALVGAPPPSPEHLE
                     RIIVDLTERLGR"
     gene            complement(2972..4474)
                     /locus_tag="PKY13_07940"
     CDS             complement(2972..4474)
                     /locus_tag="PKY13_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT79902.1"
                     /translation="MAPDTAAARRRWFALGVLVLPVLIISMDATVLGFAVPQLSEALE
                     PSSSQLLWIIDIYSFVLAGLLVTMGALGDRIGRRRLLTIGAGAFSVASLVSAFSTSPE
                     MLIGARALLGIAGATLMPSTLSLIRNIFEDDRERQTAIALWAASFAVGTALGPIVGGF
                     LLGHFWWGSVFLAGVPVTGALLVAAPRLVPESKDPQPGAFDLTSAALSMLTMIPSVYA
                     VKMFAEHGPTASAAVALVVGVVSGVLFVRRQRRLTDPMIDVTLFRVPKFRMAVSGNLL
                     ACFGFAGTLFIVTQYLQLVSGMSPLRAGLQLLPALVASMAMMAAAPAAARRFGPFAVI
                     AVGLTMGAVGFALLTQLGTDSVPLVTVAVLVLNGGLGMAMSVAVDGILASVPPARAGA
                     GASVSETANELGIALGTALLGSIMTAVYRSRMELLDAVPDDALRRARETLGAAIVASE
                     ELSGAAGAALESGARSAFVEGVHTAAIVASAATFVVAVWAAVTRLRDGGD"
     gene            complement(4566..5315)
                     /locus_tag="PKY13_07945"
     CDS             complement(4566..5315)
                     /locus_tag="PKY13_07945"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT79903.1"
                     /translation="MMIFGVVNCSPDSLNTESFVGDEREASRRIDALLSTGADGIDIG
                     GQGSTEIATLTDWQTEWDRVAPAIRAATARTSDVSIDTFRVEVARAAFEAGVTVLNAA
                     NGMQDDTFWELAAEFGTSIVVPFMNGPNPLELEHVVGDPLDAMIDYFGDRLSTADRFG
                     VRDRCWLDPGTGFGPHGWAWEDRYHYQKAVYSGLDRLRVLDRPLYIPLPWRETPQHEE
                     LLEIVLAQRPEYGRAHYPDRIRAVEERLTAS"
     gene            5314..6216
                     /locus_tag="PKY13_07950"
     CDS             5314..6216
                     /locus_tag="PKY13_07950"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HOT79904.1"
                     /translation="MGENLTVGTTRRDHVDVDVDLASILRRKGSRTVSVCLPCRNEEA
                     SVGRVVESLLPDGDARNLIDELIVIDDRSTDRSAERALAAGAKVVSIDEIENAHGPTV
                     RPGAGKGNVLWSSLWCTTGDLVVWIDADVETVDVTWVARLVEPLLDDDGVGLVKASYH
                     RPQDAGGGGRTTELVVRPLLSLLAPGLSWLHQPLGGEVAVRRTMIEQLPIAQGWGVEI
                     GMLLDVVERFGPGAIVQVDLGERRHRHHDLLDLRVQAAEVAATILRRTGVDIGELPQL
                     LVPDGPAVDLNLADRPPVASLRHG"
     gene            complement(6234..6818)
                     /locus_tag="PKY13_07955"
     CDS             complement(6234..6818)
                     /locus_tag="PKY13_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79905.1"
                     /translation="MTHFTAIGRLTKRGPVVIATSAVLVAGALAAGITAASASGSADD
                     GTPDRGRGDVIVPAAPATVPTTPTTTVAAVPSTPATDDDGTPDQGRGDAPVPAVPAPD
                     SSAPAVTVAPVPAPAPASGGSTDDDGTPDQGRGDRPSTTAAPVPSPTVTIDDHGGDRD
                     RGADHDSDDDGSGRGRGRGRGGDDDSSGHGSGHD"
     gene            complement(6900..7619)
                     /locus_tag="PKY13_07960"
     CDS             complement(6900..7619)
                     /locus_tag="PKY13_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79906.1"
                     /translation="MSDHDDLDDALRQRMQAGIRGGGDAHHELSSMRPRFERARRRRA
                     TVTAAASTAAVAIVVVGALSLGGGPRDERVQVADSSVAPRSTTTTSTTTTVAPTSAAP
                     TSPPTSADPGPSITTPTPAPTPTADGGTNPGTSPVPATSTPPANPLPTVTSAVPAPTT
                     ATPGGQRVLSSDGGTATVTWTATSLVVDRTDPAAGWTVEQVERKEPTRVVVKFRRDDG
                     GPGSSSATIDARVVAGRLEVN"
     gene            complement(7616..8167)
                     /locus_tag="PKY13_07965"
     CDS             complement(7616..8167)
                     /locus_tag="PKY13_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT79907.1"
                     /translation="MSGAGEDAVVRAVPDFDALFLREHARLVRVVAVVSGDPEAAADC
                     VADAFERAYVRWRRVGRLDDPAGWVRRVAINRAIDQQRRRSTGAVALRRLAGRADASR
                     TAELEWSDSAAFHDSELATAVAGLSPQQRAVVALHYLDDLAVADVASTLRLSEGAVKY
                     HLHQARARLRVALADLPDPIEAP"
     gene            8421..9005
                     /locus_tag="PKY13_07970"
     CDS             8421..9005
                     /locus_tag="PKY13_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOT79908.1"
                     /translation="MSVKYLLQAFATVFPAELPDKTMIATIILVTRYRRPGWVWLGAV
                     SAFTIHVIVAVAAGSAISLLPETLVKVVVATLFAVGAVLLFRSARNPEEEDTDTEIVT
                     GATVRATVLGSFGLILLAEWGDLTQLATASLAAKSGAPISTGVGALLALACVAGIAAT
                     FGRQIVARFPIERINYVGAAVFAGLAVWTVVELV"
     gene            complement(9024..9146)
                     /locus_tag="PKY13_07975"
     CDS             complement(9024..9146)
                     /locus_tag="PKY13_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79909.1"
                     /translation="MAFPLLRVVAICAGAGYTYWRLRFDERETQRVAARDARQD"
     gene            complement(9210..9494)
                     /locus_tag="PKY13_07980"
     CDS             complement(9210..9494)
                     /locus_tag="PKY13_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOT79910.1"
                     /translation="MLIIAGHLVVDPAHRDTYVADCVPVVAAARVAPGCLDFSVTADT
                     VDTAVVRIFERWETEASLVAFRGTGAEADRQIQMLAADVRSYEVASVGEP"
     gene            complement(9588..>10124)
                     /locus_tag="PKY13_07985"
     CDS             complement(9588..>10124)
                     /locus_tag="PKY13_07985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT79911.1"
                     /translation="AALSGSDGPFALMRGEIPALPAGWDELHRGRGHQLTVHVDAYVA
                     APRPDVRRLTVDDVPQMLDLVAVTEPGPFRPRTIEMGRYYGHFDGARLVAMAGERLHL
                     DGFTEISAVCTHPDARGRGLASGITGVVAEGIFERGEQPFLHVAENNAGALRVYEGLG
                     FVRRRWVEFALIQASSGH"
BASE COUNT         1455 a         3589 c         3625 g         1455 t
ORIGIN      
        1 cgtcgtggac gcccgcgggg tcacggtgcg ctacggcaag gtcgtcgcgg tgcgggaggt
       61 cgacctccgc ctcgccgagg gcgagatcgt cgcgctgatg ggccgcaacg ggtcgggcaa
      121 gtcgtcgctg atgtgggcgc tgcaggggtc ggggccccgc gccgggggca tcgtgtcggt
      181 gaccggatcc gacggcgtcg cggtcgatcc cggcgcgctc aaggccggcg aggcgcgccg
      241 gcgggtcggt ctcgtgccgc agaccccggc cgacctcctc tacctcgcga ccgtcgcggc
      301 cgagtgcgcg cgggcggacg ccgacgccgc ccagcccgcc gggtcgtgcg cggcgctcct
      361 gggtcggctc gtcggcgaga tcgacgacac catccatccc cgtgacctgt cggagggcca
      421 gcgcctggcg ctcgtgctcg ccatccagct cacggccgcg ccccgggcca tgctgctcga
      481 cgagccgact cgaggcctcg actacgacgc caaggccggt ctcggcgcgg tgctccgtga
      541 actggccgac gaggggcgtt cgatcatggt gtcgacccac gatgtggagt tcgtcgcatc
      601 ggtcgccgac cgcgtcgtgg tgatggccca gggcgaggtg gtcgccgacg gcacgacggc
      661 cgaggtggtc gtcgcgtcac cgacgttcgc gccgcaggtt gcaaaggtgc tggctcccga
      721 gccctggctg accgtcgagg ccgtcgccgc agcgctcgcc cgaaccgagg cgccgacgtg
      781 accacccaga cgatccccga cacgatcccc gaccgccgtc acgacgtgat gcgcctccgc
      841 gcccgggccg tgatggtgct gtcgctcgcc tcggtcgccg gcctctgcat gttcctgtgg
      901 ccgctgctcg tcgtcccgcc gaccgagttc tcccacaaca ccgacgcgcc gttcgtcttc
      961 gtgctgatca tgccggtggt cgtcgccgtg gtcctgtcgg agctgagcag cggcggcatg
     1021 gacacgaagt cgctggcgat gctcggcgtg ttgtcggcga tcggcgccgc gttgcgcccg
     1081 atgggcgccg gggtcgccgg catcgagacg gtgttcttca tgctggtgct cgccggccgt
     1141 gtctacggtc ccgggttcgg gttcgtgctc ggcaacacga cgctgttcgc ctcggcactg
     1201 ctcaccggtg gggtcggccc gtggttgccg ttccagatgc tggcatcggc gtgggtcggg
     1261 ctcggcgccg ggctcctgcc gtggccccgg atccgcggac gggccgagat cgcgctcctc
     1321 gccgcgtacg gggccgtcgc tgcgtacgcg ttcggcttcc tgatgaacat gtggttctgg
     1381 ccgtactcga tcggcgaggg taccgagctg tcgttcgtac cgggcgatcc gatctggtcg
     1441 aacctgcacc gattcgccct cttcaccctg gcgagctcga cgtggggctg ggacaccggt
     1501 cgcgccatca cgaatgtcgt ggccgtcgcg ttgctggggt cgacggtgct cgcgacgcta
     1561 cggcgggcga cacgcaaggc gaacttcggg gccccggtga cgttcacggc agcccacgcc
     1621 acggctgacg acggcgggtc gtcgccgaac cggggttgaa caacacctgc ccgtcgacac
     1681 cgacggtatc gccacgtgcg tgtcggcgag caccagaacg gtcagcgggc ggtccacgcc
     1741 ctcggttcgg tgcagtcgga ccgcacgtca ggtgggggcg tcgtcggcga ggacgagctc
     1801 gaaccagacg accttgccgt cggtccgctc ctcgacgccc cacttcgtcg acatctggtc
     1861 gacgatcgcc aggccgtacc cgcccgggtc gagcgggtcg agcgacgaac cgaggcgggg
     1921 caggtccgtc gacgtgtcgt ggacctcgac gcggacggcg tctccgtcgc ttgccagggt
     1981 gatcagagcg gaggacaggc aggcatgggt gatggcgttg gtcacgagct ccgacgtcag
     2041 caactcggcg gtgtcgacgg ccacggtcgg cgcattcatc agtgcgtcgc ggacgaagtg
     2101 gcgggcatcc gacggggtct gggcccggta cacgagctcg atggtcatca ctcagaccgg
     2161 tgcccccttc gcaggtggcc gagacacatc atcgacagaa cctgcggtcg gccccccacc
     2221 ccgggcgccg aatcaccgag cagccgattg cccctcagac tccgggacgg ccggcagcgc
     2281 gctgctcgtc gggcggatac gggcagtggc gacagccatt gccgcagcag taaccacgat
     2341 cgagcaggta caccgacgtc atcactgcgt accccgttgc cgggtcgacg taggtcgggg
     2401 cgccggcggc gacggctcga tcgtgcgctg cgacggcctg cggatcgatc atcggccgag
     2461 gcgctcggtg aggtcgacga tgatccgctc gaggtgctcg ggcgacggcg gcggagcacc
     2521 gacgagcgcg ttgaggtaga gcccgtcgcc gatcaaccgc acctgctcgg cgaggaacgg
     2581 atcgagcacc gccaagggcc gggtgaagcg cacgaaggcg tcgctgatcg cctgggcgag
     2641 cccgtcgtcg acggcgtgga tcgcggcgag gagcgaacgc cacaccgcca gctccacggg
     2701 gtcggacgga tcgtaggaga ggtaccagcg gatcagctcg gcgggctcgt ccggcgctgc
     2761 ggcaacgatc tcgtcgaccc gtgtcatcag gcgctcgacg aggccggcga gcagcgccgc
     2821 cttcgacggg aagtggtaga tcaacccgcc cttggagatg ccggcgcgtt cggcgacggc
     2881 atcgagcgtc gcgctgccgg cgccgtcgtc gaggaggacc tgttcaaagg tgtcgaggat
     2941 gcgttcacgg ctggacggtc gggccacggc gtcagtctcc cccatcacgg agccgggtca
     3001 ccgcagccca caccgcgacg acgaaggtcg ccgccgacgc cacgatcgcc gcggtgtgca
     3061 ctccctcgac gaaggccgac ctcgctcccg actccagcgc ggcgccggcc gcgcccgaca
     3121 gctcctccga cgccacgatc gcggcgccga gggtctcgcg ggcccggcgc aacgcgtcgt
     3181 cggggaccgc gtcgaggagc tccatcctcg accggtagac cgccgtcatg atgctgccga
     3241 gcagcgcggt gccgagggcg atgccgagct cgttcgcggt ttccgacacc gaggctccgg
     3301 cgccggcccg cgccggcggg accgacgcca agatgccgtc gaccgcgacc gacatggcca
     3361 tcccgaggcc gccgttgagc accaacaccg cgacggtgac cagcgggacc gagtcggtcc
     3421 cgagttgcgt gaggagcgcg aacccgacgg ctcccatcgt gagcccgacg gcgatcacgg
     3481 cgaacggccc gaaccgccgg gcagccgccg gcgccgcggc catcatcgcc atcgatgcca
     3541 cgagggccgg gagcagctgc agtccggccc gcagcggcga catgcccgac accagctgga
     3601 ggtactgcgt cacgatgaac aacgtgcccg cgaacccgaa gcacgcgagc aggttgcccg
     3661 acacggccat ccggaacttc gggacgcgga acagcgtcac gtcgatcatc gggtcggtca
     3721 gtcgccgctg acgccgaacg aacagcactc cggagacgac gccgaccacc agcgccactg
     3781 cagcgctcgc cgtcggaccg tgttcggcga acatcttcac cgcgtagacg ctcgggatca
     3841 tcgtcagcat cgagagggcg gcgctggtga ggtcgaacgc gcccggctgc ggatccttcg
     3901 attcgggcac caggcgcggc gccgccacga gcagtgcgcc ggtgacgggg accccagcga
     3961 ggaacaccga gccccaccag aagtggccga ggaggaagcc gccgacgatc ggcccgagcg
     4021 ccgtgccgac ggcgaacgat gcggcccaca gagcgatcgc ggtctgacgc tcacggtcgt
     4081 cctcgaagat gttgcggatc agcgagaggg tcgagggcat cagggtcgcc ccggcgatcc
     4141 cgagcaacgc ccgggcgccg atcagcatct ccgggctcgt cgagaacgcc gagacgagcg
     4201 acgcgacgct gaacgccccc gcaccgatcg tgaggagtcg ccggcgaccg atgcgatcgc
     4261 cgagggcgcc catcgtgacg aggagccccg ccaggacgaa cgagtagatg tcgatgatcc
     4321 acagcagttg cgagctggac ggttcgagcg cctcggagag ttgtgggacg gcgaacccga
     4381 ggacggtggc atccatcgag atgatgagca ccgggagcac gagcaccccg agcgcgaacc
     4441 agcgtcgtcg agccgctgcg gtgtcgggag ccatcggata actgtaccaa ccagtcggta
     4501 cagtattccg gggttttgta ctgcgaggtg cgccagatgc ggcgcgtctc gcagtacaga
     4561 acccctcagc tggcggtgag ccgctcctcg accgcccgga tccggtccgg gtagtgggcg
     4621 cggccgtact cgggacgctg ggcgaggacg atctcgagga gttcctcgtg ctggggcgtc
     4681 tcccgccacg gcaacgggat gtacagcgga cggtcgagga cgcgcagtcg gtcgaggccc
     4741 gaatacacgg ccttctggta gtggtagcgg tcctcccacg cccagccgtg gggcccgaag
     4801 ccggtgccgg ggtcgagcca gcagcgatcc cgcaccccga accggtcggc ggtcgagaga
     4861 cggtcgccga agtagtcgat catggcgtcg agcgggtcgc cgacgacgtg ctcgagctcc
     4921 aggggattcg ggccgttcat gaacggcacc acgatcgagg tcccgaactc ggcggcgagc
     4981 tcccagaacg tgtcgtcctg cataccgttc gcggcgttga gcacggtgac accggcttcg
     5041 aacgccgccc gtgcgacctc gacgcggaac gtgtcgatgc tcacgtcgga ggtgcgtgcc
     5101 gtggccgccc ggatcgccgg ggccacgcgg tcccactcgg tttgccagtc ggttaacgtc
     5161 gcgatctccg tgctcccctg accaccgatg tcgatcccgt cggcaccagt gctgaggagt
     5221 gcgtcgatgc gccgggaggc ctcccgttca tcacccacga acgattcggt gttgagcgaa
     5281 tcgggagagc aattgacgac tccgaagatc atcatgggcg agaacctaac ggtcggaacg
     5341 acgcgccgcg accacgtcga tgtcgatgtc gacctggcgt cgatcctccg ccgcaaggga
     5401 tcccgcaccg tgtcggtgtg cctgccgtgc cgcaacgagg aggcttcggt cggtcgcgtc
     5461 gtcgagtcgc tccttcccga cggcgacgcc cgcaacctga tcgacgagct gatcgtcatc
     5521 gacgaccggt ccaccgatcg ctccgccgag cgggcgctcg ccgccggcgc caaggtcgtg
     5581 tcgatcgacg agatcgagaa cgcccacggc cccaccgtcc gccccggggc cggcaagggc
     5641 aacgtgttgt ggtcctcgct ctggtgcacg accggcgacc tcgtcgtgtg gatcgacgcc
     5701 gacgtcgaaa ccgtcgacgt cacgtgggtc gcccgcctcg tcgaaccact cctcgacgac
     5761 gacggtgtcg gtctcgtcaa ggcgtcctac caccgacccc aggacgccgg cggcggaggt
     5821 cgcaccaccg agctcgtcgt ccgcccgctg ctctcgctgt tggctcccgg cctgagctgg
     5881 ctccaccagc ccctcggcgg cgaggtcgcg gtgcggcgca cgatgatcga gcagctgccg
     5941 atcgcccagg gctggggtgt cgagatcggc atgctgctcg acgtcgtcga acggttcggt
     6001 ccgggcgcca tcgtgcaggt cgacctcggc gagcgccggc accgtcacca cgacctcctc
     6061 gacctgcggg tccaggccgc cgaggtcgca gcgacgatcc ttcgccgaac cggcgtcgac
     6121 atcggcgagc tgccgcagct cctcgtcccc gacggtccgg ccgtcgacct caacctcgcc
     6181 gaccgcccgc cggtcgcgag ccttcgccac ggctgaacgt tcgggcctcc cggtcagtcg
     6241 tgaccggagc cgtgtcccga gctgtcgtcg tcgcccccac gtccgcgacc gcggccacga
     6301 ccggagccgt catcgtcgga gtcgtggtcg gcgcctcggt ctcggtctcc accgtggtcg
     6361 tcgatcgtga cggtgggtga cggcaccggc gccgccgtgg tggaggggcg gtcgccccgg
     6421 ccctgatcgg gcgtgccgtc gtcgtcggtc gagccgccgc tggcgggagc gggagcggga
     6481 accggcgcca cggtgaccgc cggcgccgac gagtcgggag cgggcacggc cggcaccggc
     6541 gcgtcgcccc gaccctggtc gggggtgccg tcgtcgtcgg tcgccggagt cgaggggacg
     6601 gcggcgaccg tggtggtcgg cgtcgtcgga acggtcgccg gtgccgcggg gacgatgacg
     6661 tcgccccggc cccggtcggg agtgccgtcg tcggcgctgc cgctggcgct cgctgcggtg
     6721 atgccggcgg cgagggcccc ggcgaccagc accgcgctcg tggcgatcac caccgggccc
     6781 cgcttcgtca gtcgtccgat tgccgtgaag tgcgtcatgt cgatcctctc gtcgggcggc
     6841 tcgcgtcgag ccgacgaaga gggacacggg tgacccggtc ccgaggtttg gccgacgcct
     6901 cagttcacct cgagccgacc ggcgaccacg cgtgcgtcga tcgtcgcgga gctggagccc
     6961 ggccccccgt cgtcgcggcg gaacttcacg acgacacgcg tcggttcctt gcgctcgacc
     7021 tgctccacgg tccacccggc ggccgggtcg gttcggtcga cgacgaggga cgttgcagtc
     7081 caggtcaccg tcgccgtgcc gccgtcggac gacaggaccc gctgtccgcc gggcgttgcc
     7141 gtcgtcggcg ccggcacggc cgacgtgacg gtcggcaggg ggttcgccgg cggcgtcgac
     7201 gtcgccggga ccggcgaggt gccggggttg gtcccgccgt ccgccgtcgg cgtcggcgcc
     7261 ggcgtcggcg tcgtgatcga cggcccgggg tcggcggacg tcggagggct cgtcggcgct
     7321 gcgctggtgg gagcgacggt ggtcgtcgtc gaggtcgtcg tcgtggaccg cggcgcgacc
     7381 gacgagtcag cgacctggac gcgttcgtcg cgagggccac cgcccagcga caacgctccc
     7441 acgacgacga tcgccaccgc cgccgtcgac gctgccgcgg tgaccgtcgc acgccggcgg
     7501 cgcgcccgtt cgaaccgagg ccgcatcgac gacagttcgt ggtgcgcgtc gccgccccca
     7561 cgaatgccgg cctgcatccg ctgccggagt gcatcgtcga ggtcgtcgtg gtcgctcatg
     7621 gtgcctcgat cgggtcggga aggtcggcca gtgctacccg gaggcgggcc cgggcctggt
     7681 gcaggtggta tttgacggcg ccctcgctca accggagcgt cgacgcgacg tcggcgaccg
     7741 cgaggtcgtc gaggtagtgc agtgcgacga cggcgcgttg ttggggactc agtccggcga
     7801 ccgcggtcgc cagttcgctg tcgtggaacg cggcggagtc cgaccactcg agctcggcgg
     7861 tccgcgacgc gtcggcgcgg ccggcgagac gccgcagggc cacggcgccg gtggagcgac
     7921 gacgctgctg gtcgatggcc cggttgatcg ccacgcgccg gacccaccct gcggggtcgt
     7981 cgagccggcc cacccggcgc cagcgcacgt aggcgcgctc gaacgcgtcg gccacgcagt
     8041 cggcggccgc ctcgggatcg ccggacacga ccgcgacgac ccggacgagg cgggcgtgct
     8101 cccgcaggaa gagcgcgtcg aagtcgggca ctgccctcac cacggcgtcc tcaccggcgc
     8161 ccgacatcca cgacacggtc gtcattcaat cgggtgacac gcacggggcc gcgccaaggt
     8221 ttggtagcgt ctcgtcgaac tgaaggggag tagcccccaa tccgtcgatc gacacactgg
     8281 cccgccgacc tctcgtcgtc ggacccggtc ggcggggcct gtgcgagcag gcgggcgaga
     8341 ccttcggccg aacgaccgct gtcgttccgg ccggaggagt ctgcctctga tggtcgggac
     8401 ccgaaaccca ccgaggacct atgtccgtca agtacctgct ccaggccttc gccaccgtct
     8461 tccccgccga actccccgac aagacgatga tcgcaacgat catcctcgtg acgcgctacc
     8521 gccgtccggg ctgggtctgg ctcggcgccg tgagcgcgtt caccattcac gtcatcgtgg
     8581 ccgtcgccgc ggggagcgcc atcagcctgc tccccgagac gctcgtgaag gtcgtggtcg
     8641 ccaccctgtt cgcggtcggc gccgtcctcc tgttccgctc cgcccggaat cccgaggagg
     8701 aggacaccga caccgagatc gtcacgggcg ccacggtccg agcgacggtg ctcggcagct
     8761 tcggcctgat cctgctcgcc gaatggggcg acctcaccca gctcgcgacc gccagcctgg
     8821 ctgccaagag cggtgcgccg atctcgaccg gcgtcggggc gctgctcgcc ctggcctgcg
     8881 tcgccggcat cgctgccacg ttcggccgtc agatcgtcgc ccgcttcccc atcgagcgca
     8941 tcaactacgt cggcgcggcg gtgttcgccg gcctcgccgt gtggaccgtc gtcgaactgg
     9001 tctgacgccg acgcggcggg gcgtcagtcc tgacgggcgt cgcgtgccgc aacccgctgc
     9061 gtttcacgtt cgtcgaaccg gaggcgccag taggtgtacc cggctccggc gcagatcgcg
     9121 acgactcgca acagtgggaa ggccatgtcc tctcctcggc agggcagtca acgatcttga
     9181 gcttctccga tcgggacggc gaatgggcgt cagggctccc cgaccgacgc cacctcgtag
     9241 ctccgcacgt cggcggcgag catctggatc tgccggtcgg cctcggcgcc ggtgccgcgg
     9301 aacgcgacga gcgaggcctc tgtctcccac cgctcgaaga tgcgcaccac agcggtgtcg
     9361 accgtatcgg cggtgaccga gaagtcgaga cagcctggcg caacgcgcgc tgctgccacg
     9421 acggggacgc aatccgcgac gtacgtgtcg cggtgagcgg gatcgacgac gagatgtccg
     9481 gcgatgatga gcatgtcggt gcagacgatc tctcggccgc caactcatcg cgtgaccctg
     9541 gcgccggggc ggggcgacct cgcgacccgg ttccgcggct gcgcccgtca gtgccccgac
     9601 gatgcctgga tcagcgcgaa ctcgacccag cggcggcgga cgaacccgag accctcgtag
     9661 acccgcagcg ctccggcatt gttctcggca acgtgcagga acggttgctc gccccgctcg
     9721 aagatgccct cggcaacgac gccggtgatg cccgaggcga ggccgcggcc acgggcgtcg
     9781 ggatgggtac agacggcgga gatctcggtg aacccgtcga ggtgcagccg ctcgcccgcc
     9841 atcgccacca gccgggcacc gtcgaagtgc ccgtagtacc ggcccatctc gatggttcgg
     9901 ggccggaacg ggccgggctc cgtcaccgcc acgaggtcga gcatctgcgg cacgtcgtcg
     9961 acggtgagcc gacgcacgtc ggggcgcggc gcggcgacgt acgcgtcgac gtgcaccgtc
    10021 aactggtgac cgcgaccacg atgcagctcg tcccacccgg ccggcaacgc tgggatctca
    10081 ccgcgcatca gcgcgaacgg cccgtcggaa ccgctcagcg cagc
//
LOCUS       DAOSOA010000097        10069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6683_length_10069_cov_7.802776, whole genome shotgun sequence.
ACCESSION   DAOSOA010000097 DAOSOA010000000
VERSION     DAOSOA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10069
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6683_length_10069_cov_7.802776"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PKY13_07990"
     CDS             <1..1271
                     /locus_tag="PKY13_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT79912.1"
                     /translation="EPVSPALVPSEPVEPEPEAVIAESEPEAVIAEPEPEPEPAPEAE
                     TLVAEPEPEALVAENEPTAAPKPVVGASAPAGTAPPPPPVVGASRPAVAVGRIVSVEV
                     VASSPSEIEVKLADGRAGVISRADFGDEPMPSPGSTIEAALLARDDPQKRVVLSRSWA
                     VKLQRWERVEAAKESGQPLTGPVTRSIKGGLIVDLGLRAFLPASMIDEHQPGDETTDP
                     ASLVGTEVTVVVTEVDRAQDRVVVSRRDHLRRQRRRTERDVFASLTVGKRVTGKVVGL
                     VEYGAHVDLGGVRALLHRSEMSWGRVNRPADVVSVGDTIETVVIEVNKSKRRVGLSLR
                     QLEADPFAGVEAGVVTTAVVTRVVEYGVFARLDDTDVVGLIHMSELTDLPGYRPDELV
                     TPGESIHVKVLSVDPKKRRVSLSVRQALWS"
     gene            complement(1338..2180)
                     /gene="purU"
                     /locus_tag="PKY13_07995"
     CDS             complement(1338..2180)
                     /gene="purU"
                     /locus_tag="PKY13_07995"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOT79913.1"
                     /translation="MTVLLLSCPDRPGLVAATAGLIHERGGDIAHADQHADDRHGLFL
                     QRIEFSFADDADHERFRVEFASLAESLGMTWEIVEPGQRARVALLVSKEGHCLLDLLA
                     RIRLGELAADVPVVISNHEDHREITEHFGVPFLHLPVDPDDKAAQQRALLAALDEVNV
                     ELVVLARYMQILPPEIIERFPRQIINIHHSFLPAFVGAKPYHQAYDRGVKIIGATAHY
                     ATAELDQGPIICQEVTPVSHRDTPERLIRLGGDLEQTVLARAVRLHLERRVLTYANRT
                     VIFD"
     gene            complement(2202..2618)
                     /locus_tag="PKY13_08000"
     CDS             complement(2202..2618)
                     /locus_tag="PKY13_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT79914.1"
                     /translation="MPVLPDEFPPHPARDASLKSMTAVEAGDREAWLDLFADDAVVQD
                     PVGVSALDPTGLGHRGRDAIGAFFDNVIGPNEVRFTLRESWEADHEVANVGRITTTMA
                     DGLVVHTDGVFTYRVDDEGKVVSLRAHWSVDRLTFG"
     gene            complement(2602..3900)
                     /locus_tag="PKY13_08005"
     CDS             complement(2602..3900)
                     /locus_tag="PKY13_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT79915.1"
                     /translation="MTTAPIEIDTSGIDLADPQFATIEAWADGSATRAAYFKKLRDDA
                     PVMFAEEAAIEGFPQGPGFWSVTRFDDIMHVSRHPELFCSGLGTNIPDLPVEIAEFYG
                     SMISMDAPRHTRLRRIVSKSFTPRMVARIDEDVHVKARGVLAEVGSKGECDFVAEIAS
                     VLPLQIICEMMGIPRESWTRIMELTNVILGGGDPELTPDFGTLLAASLEMAAIAQAVG
                     EDRLANPRDDLVSALMHASVDGESLTPQEMASFFILLAAAGNETTRNAISHGAWELHN
                     DRAQWDAWATDPETLSPTAVEEIVRWATPVIHFRRTATEDTEISGQKIAKGEKVVMWY
                     ESGNRDERHFEDPHRFDIARTPNDHIGFGAGGPHFCLGANLARREIRVMFEEIFRWLP
                     DLRITAPPDYLQSPFIHGIKRMRCEFTPADVPRLPHASPA"
     gene            4021..5685
                     /locus_tag="PKY13_08010"
     CDS             4021..5685
                     /locus_tag="PKY13_08010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT79916.1"
                     /translation="MTTSIRLRPWQKDALEKLLASDADNFLAVATPGAGKTTFALTAA
                     AHHLADRSCRGLVVVAPTQHLKLQWAESAHRLGLHLDHEWSARDGELPADMHGIVTTY
                     QQVSTSAAVLRTLSAGAFVILDEVHHAGDDKSWGESVLTAFASARRRLMLSGTPFRSD
                     TSAIPFVDYHLDEARPDFEYGYGDALTDGRVVRPVYFPAFGGHMEWVAPDGSEFSASF
                     DDALDFQRANQRLRTALSLEGQWLPKVLAEAHAQLLGIRRQHPNAGGLVIAMDQEHAQ
                     GIARLMRQRFGVSPTIAVSDDPMASAKIHAFSSSTDPWIIAVRMVSEGVDIPRLRIGV
                     FATNTTTELFFRQAVGRLVRWTKGVRSQKAFLFIPDDPRLRRWATGIAEQRRHSLKKR
                     QREDDEFGGLDGSELDDRDAMEAADNDQEQMSLFAVISAVATEESTVQNSLFSDDHPD
                     RWDDDDESDAVMDAALEVRLAVPPPPGAAGAAALPDGKTRKQLKGELRDANAELVREL
                     VRFSNLDHRAVNVELNRLAGIRKITEATVEQLQTRLDQGNKWLRKL"
     gene            complement(5813..5986)
                     /locus_tag="PKY13_08015"
     CDS             complement(5813..5986)
                     /locus_tag="PKY13_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79917.1"
                     /translation="MGTRPRMDHSIRNAMLVAALAGLASAVRRELDHGRMHQPPRIGE
                     TALGEPGEVLWYC"
     gene            6112..6513
                     /locus_tag="PKY13_08020"
     CDS             6112..6513
                     /locus_tag="PKY13_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT79918.1"
                     /translation="MNADGGAVGVLMKIGQLADAVGVGVETVRYYERRGLLEPPLRTE
                     AGYRQYGASDVARIEFLLRAKELGFTLAEISELFAATTTDVAAAVRAKLTAVEAQISE
                     LAETRCRLRQLIDLCDHGDGSACLQLRLVGS"
     gene            6543..6869
                     /locus_tag="PKY13_08025"
     CDS             6543..6869
                     /locus_tag="PKY13_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT79919.1"
                     /translation="MTTTSDLTRSIDVDELIASTELPLLLDFGADWCPPCRALDLILD
                     QIAAEHIDSIVIRKVDVDQVPDLVVRFGIASAPTMLLFVGGELEAKIVGARPKAALLE
                     MLGDHL"
     gene            6963..>8458
                     /locus_tag="PKY13_08030"
     CDS             6963..>8458
                     /locus_tag="PKY13_08030"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOT79920.1"
                     /translation="MTESPPAEPTDGGLADVSASALRVFPRHLLPAKRSEIARRATQI
                     AWVTAKHFTPLAIRSARKREVDPDAFARPLRLTFEELGTTFMKFGQLVSSSPGVFGEE
                     VSAEFRSCLDTGPAVPFEQVRRTVEAELGMHLEEAYAHFDPEPIGRASIAVVHRATLH
                     DGTDVAVKVLRPGIEKRVSIDMDLFEPMLTIIARTTGEYAAGQLVQMFDGFRMQLGEE
                     LDLRNEARAIQHMQKLLRVVELPLVTVPEVYPAFSAARVLTMEFLDGVPVDDMSRIAE
                     YGFDPAPVVSQTVKGFLMTTVRWGFFHGDVHAGNLLLLRDGRVGVIDWGIVGRLDKDT
                     HMFFRRIVEAALGDEDAWSDIAAHTIKQYGALLETGMGMNHEDTTAFMRMMLEPLLKA
                     PFGEVSLVEILNAPQAKVAEAKGLEVENRSIASIYKRFKEQRKLRLLAEAEGIYESEF
                     ERGMFLLTKALMYFERYGKMYMSDVGIFHDEAFFRAALAAPTSTLRDAP"
     assembly_gap    8459..8558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8609..9244
                     /locus_tag="PKY13_08035"
     CDS             8609..9244
                     /locus_tag="PKY13_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT79921.1"
                     /translation="MPAASKRIAPVRRSQAERRETTRAALMVAALEQLLVDGLAGFTT
                     TEVCKRAGLSQGALFKHFDTKADLLAAVTEHLFDELRAEFESTFIDLPAARRNPRSGI
                     ELLWDQMLDARLAAAYELYTAARTDTDLRAALEPVVAAHVERIHELAAALIPGVDDDR
                     RRSAVDLALLAMQGLVVQQMALPDPAQHERLRELLDGLTVVLLTNENEGTN"
     gene            9244..>10069
                     /locus_tag="PKY13_08040"
     CDS             9244..>10069
                     /locus_tag="PKY13_08040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOT79922.1"
                     /translation="MDDLKNPAVLAVPLYFALIWWEVRALTRLKAAGKDVLGYERRDT
                     WASLLIGAVSLITVGGLNLVVKILSEWLWDYRLTDLGNGILGWTVALLGWDFAYYWLH
                     RVEHESRILWSAHVNHHSSERYNLSTALRQPWTPVVLVLFFPPLALIGVRPWMIAVSG
                     GFNLVYQFWIHTEAVDKLPRWFEFVFNTPSHHRVHHGSQQQYLDRNHGGVLIIWDRLF
                     GTFEPEGERVRYGLTKNINTFNLWKIFDHELVAIGRDVKAAPRWRDRFGYVFFGPGW"
BASE COUNT         1543 a         3348 c         3422 g         1656 t
ORIGIN      
        1 ccgagccggt gtcgccggct ctcgtgccgt ccgaacccgt cgagccggaa ccggaggcgg
       61 tcatcgccga atcggaaccg gaggcggtca tcgccgagcc cgagcccgag cccgaacctg
      121 cgcccgaagc ggagacgctc gtcgccgagc cggaaccgga ggcgctcgtc gccgagaacg
      181 agccgaccgc tgcgcccaag ccggtcgtcg gcgcctcggc acccgccgga acagcgcctc
      241 cgcctccgcc ggtggtcggg gcgtcgcgcc cggccgtcgc cgtcggacgc atcgtgtcgg
      301 tcgaggtggt cgcgtcgagc ccgtcggaga tcgaggtgaa gttggccgac ggccgagcgg
      361 gggtcatctc acgagccgac ttcggtgacg agccgatgcc gtcgccgggg tcgacgatcg
      421 aggcagcgtt gttggcgcgc gacgaccccc agaaacgggt cgtcctcagc cgttcgtggg
      481 ccgtgaagct ccagcgatgg gagcgggtcg aagccgcgaa ggagagcggt cagccgctca
      541 ccggaccggt gacacgaagc atcaagggcg ggctcatcgt cgacctcggc ctccgggcct
      601 tcctgccggc gtccatgatc gacgagcacc agcccggcga cgagaccacc gatccggcct
      661 cgctcgtcgg gaccgaggtg accgtcgtgg tgaccgaggt cgaccgggcc caggaccgtg
      721 ttgtcgtctc gcgtcgcgat catctgcgtc gtcagcgtcg tcgtaccgag cgcgacgtgt
      781 tcgccagcct caccgtgggc aagcgcgtca ccggcaaggt ggtcgggctc gtcgagtacg
      841 gcgcccacgt cgacctcggc ggagttcggg cgctgctgca ccgcagcgag atgtcgtggg
      901 ggcgggtcaa tcggcccgcc gacgtcgtgt ccgtcggcga caccatcgag accgtcgtca
      961 tcgaggtcaa caagtcgaag cggcgggtgg ggctgtcgct ccgtcagctc gaagccgacc
     1021 cattcgccgg ggtcgaggcc ggtgtcgtca ccacggcggt ggtcacccgt gtcgtcgagt
     1081 acggcgtgtt cgcccgcctc gacgacaccg atgtcgtcgg gctcatccac atgagcgagc
     1141 tcaccgacct gcccggctac cggcccgacg agctggtgac gccaggcgag agcatccacg
     1201 tgaaggtgct ctcggtggac ccgaagaagc ggcgcgtgag cctctcggtt cgtcaggcac
     1261 tctggtcctg acgcctcggg ctttgtactg cgaggaacgc cacgagcggc gctcctcgca
     1321 gtacacaacc cgtgaggtca gtcgaagatg acggtgcggt tggcgtaggt cagcacgcgg
     1381 cgttcgagat ggaggcgcac cgcccgggcc agcaccgtct gctcgagatc gccgcccagc
     1441 cggatcagtc gttcgggggt gtcgcggtgg ctgaccggcg tgacctcctg gcagatgatc
     1501 ggcccctggt cgagctccgc ggtcgcgtag tgcgctgtgg ccccgatgat cttgaccccg
     1561 cggtcgtacg cctggtggta cggcttggcg ccgacgaacg ccggcaggaa cgagtggtgg
     1621 atgttgatga tctgccgggg gaaccgttcg atgatctcgg gcggcaggat ctgcatgtag
     1681 cgagcgagca cgaccagctc gacgttcacc tcatcgagcg ccgcgaggag cgcccgctgc
     1741 tgggccgcct tgtcgtcggg atcgacggga agatgcagga acggcacccc gaagtgctcg
     1801 gtgatctcgc ggtggtcctc gtggttcgag atgacgacgg ggacgtcggc ggcgagttcg
     1861 ccgaggcgga tccgtgccag gaggtcgagg aggcagtgcc cctccttcga cacgagcagc
     1921 gcaaccctcg cccgttgtcc aggctcgacg atctcccacg tcatgcccaa cgactcggcg
     1981 agcgacgcga actcgacgcg gaatcgctcg tggtcggcgt cgtcggcgaa cgagaactcg
     2041 atgcgctgca ggaacaagcc gtggcggtcg tcggcgtgct ggtcggcgtg ggcgatgtcg
     2101 cccccacgtt cgtggatgag gccggcggtc gcggccacca gaccggggcg gtcggggcag
     2161 gagaggagga ggacggtcat cccggtttca atcggtcgtg atcacccgaa cgtgagccgg
     2221 tcgacggacc agtgggcccg cagcgacacg accttgccct cgtcgtcgac ccggtacgtg
     2281 aacacgccgt cggtgtgcac cacgaggccg tcggccatcg tggtcgtgat gcggccgacg
     2341 ttggcgacct cgtggtcggc ttcccacgac tcgcggagtg tgaaccggac ctcgttgggg
     2401 ccgatcacgt tgtcgaagaa cgcaccgatg gcgtcgcggc cgcgatggcc gagcccggtc
     2461 gggtcgagtg cggacacgcc gacggggtct tggacgacgg cgtcgtcggc gaagaggtcg
     2521 agccaggctt cgcgatcgcc cgcctcgacg gcggtcatgg acttgagcga tgcgtcgcgg
     2581 gcggggtgcg gggggaactc gtcaggcagg actggcatgg ggcaagcgtg gcacgtcggc
     2641 gggggtgaac tcacaccgca tccgcttgat gccgtggatg aacggtgact gcaggtagtc
     2701 gggcggcgcc gtgatgcgaa ggtcggggag ccaccggaag atctcctcga acatcacgcg
     2761 gatctcgcgg cgggccaggt tcgctccgag acagaaatgg ggaccgccgg caccgaaccc
     2821 gatgtggtcg ttgggcgtgc gggcgatgtc gaagcggtgg ggatcctcga agtgacgctc
     2881 gtcgcggttg cccgactcgt accacatgac caccttctcg cccttggcga tcttctggcc
     2941 gctgatctcg gtgtcctcgg tcgcagtgcg gcggaagtgg atgaccggag tggcccaccg
     3001 gacgatctcc tcgacggcgg tgggggagag cgtttcgggg tcggtggccc aggcgtccca
     3061 ttgcgctcgg tcgttgtgga gctcccaggc gccgtggctg atcgcgttgc gcgtcgtctc
     3121 gttgccggcc gccgccagga ggatgaagaa actcgccatc tcctgcggtg tcagcgactc
     3181 gccgtcgacc gacgcgtgca tgagcgcgga gacgaggtcg tcgcgggggt tggcgagccg
     3241 gtcctcgccg acggcctggg cgatggcggc catctcgagc gatgcggcga ggagcgtgcc
     3301 gaagtcgggg gtgagctcgg ggtcgccacc gccgaggatc acgttcgtga gctccatgat
     3361 ccgggtccac gactcgcgag ggatccccat catctcgcag atgatctgga gggggaggac
     3421 cgacgcgatc tcggcgacga agtcacactc gcccttgctg ccgacctcgg cgagcacgcc
     3481 gcgggccttc acgtgcacgt cctcgtcgat gcgggcgacc atgcgcgggg tgaacgactt
     3541 cgagacgatt cggcggagcc tggtgtggcg gggagcatcc atgctgatca tcgagccgta
     3601 gaactcggcg atctcgacgg gcaggtcggg gatgttggtg ccgagccccg aacagaacag
     3661 ttcggggtgg cgggacacgt gcatgatgtc gtcgaagcgg gtcaccgacc agaacccggg
     3721 gccctgcggg aacccttcga tggcggcttc ctcggcgaac atcaccggag cgtcgtcgcg
     3781 gagcttcttg aagtaggcgg cgcgggtggc ggagccgtcg gcccacgcct cgatcgtcgc
     3841 gaactgtggg tcggcgagat cgatcccgct cgtgtcgatc tcgatcggag ccgtcgtcat
     3901 cgtggtcccc cctggtccgt gatgcagcgc ccaccctagc cctctccctt gaccgaacgg
     3961 tcaaggacac gcgacccgga tgatctgggt gtcggtggcg gctgcaagaa tgttccgccc
     4021 atgacgacgt cgatccggct ccggccgtgg cagaaggatg cgctcgagaa gctgttggcg
     4081 agcgatgccg acaacttcct cgcggtcgcc acgcccggcg ccggcaagac gacgttcgcg
     4141 ctcacggcgg cggcccacca cctggccgat cgcagttgtc gaggtcttgt tgtcgtcgct
     4201 ccgacccaac acctcaagct gcagtgggcg gaatcggcgc atcgcctggg cctgcacctc
     4261 gatcacgagt ggtcggcgcg cgacggcgag ctccccgccg acatgcacgg catcgtcacc
     4321 acctaccaac aggtgtccac gtccgcggcg gtgctccgca ccttgtcggc gggcgcgttc
     4381 gtgattctcg acgaggtgca ccacgccggc gacgacaagt cgtggggcga gagtgtgctc
     4441 accgcgttcg cttccgcacg ccgtcggctc atgctctccg gcacgccgtt ccggtccgac
     4501 acctcggcca tcccgttcgt cgactaccac ctcgacgagg cccgccccga cttcgagtac
     4561 gggtacggcg acgccctcac cgacggtcgg gtggtccgtc cggtctactt cccggcgttc
     4621 ggtgggcaca tggagtgggt ggcccccgac ggctccgagt tctcggcgtc gttcgacgac
     4681 gcgctcgact tccagcgggc caaccagcga ttgcgcacgg cgctgtcgct cgagggccaa
     4741 tggctgccca aggtcctcgc cgaggcccac gcccagttgc tcggcatccg ccgtcagcac
     4801 cccaacgccg ggggccttgt catcgccatg gaccaggagc acgcgcaggg catcgcgcgg
     4861 ctcatgcggc agcgcttcgg cgtcagtccc accatcgccg tgtccgacga tccgatggcc
     4921 tcggccaaga tccacgcgtt ctcgtcgtcg accgacccgt ggatcatcgc ggtccgcatg
     4981 gtgtcggagg gtgtggacat cccccggctc cgcatcggcg tgttcgccac caacacgacg
     5041 actgagctgt tcttccggca ggccgtcggt cggctcgtgc gctggaccaa gggcgtccgg
     5101 tcgcagaagg cgttcctgtt catccccgac gatccccgtc tgcgccgctg ggccacgggc
     5161 attgccgagc agcgtcgcca ttccctgaag aagcgccaac gcgaagacga cgagttcggt
     5221 ggcctcgacg gcagcgaact cgacgaccgc gatgccatgg aggccgccga caacgaccag
     5281 gagcagatgt cgctgttcgc cgtcatctcc gccgtcgcga ccgaggagtc gacggtgcag
     5341 aactcgctgt tcagcgacga ccatcccgat cgctgggatg acgacgacga gtccgacgcc
     5401 gtgatggacg ctgccctcga ggttcgcctc gccgtgccgc cgccacccgg tgcggccggt
     5461 gccgccgcgt tgcccgacgg caagacccgc aagcagctca agggcgagct gcgtgacgcc
     5521 aacgccgaac tggtccgtga gttggtgcgg ttctcgaacc tcgaccaccg ggccgtcaac
     5581 gtcgaactca accggctggc gggcatccgc aagatcaccg aggcaaccgt cgagcagttg
     5641 cagacgcgcc tcgatcaggg caacaagtgg ctgcgaaagc tgtgagctga ccatccgtga
     5701 cccggatcgg gcgaggtcga cgaagtcgcg cctcgccccc gtcaggaccg ttcgttcgtc
     5761 ggcgaagctg acaagaattc cggagccacc gacgtgactg atgcgctgac cttcagcagt
     5821 accagagcac ttcgcccggc tcgccgagcg cggtctcgcc gatgcgcggc ggctggtgca
     5881 tcctgccgtg atcgagctcg cggcgcactg ctgacgccaa gccggcgagc gcagcgacga
     5941 gcatcgcgtt gcggatcgag tggtccattc gtggccgtgt gcccatggcg tccccctgct
     6001 cggttcggcg cctgcccgac gccccgttcc caactggtac agatctgact cgtcgagagc
     6061 aagagatttc ggcttgaccc tggaccatgg tccggggtct accgtcgtcg tgtgaacgca
     6121 gatggaggtg ccgtgggcgt gctcatgaag atcgggcaac tcgccgatgc ggtcggcgtc
     6181 ggtgtcgaga cggtgcgcta ctacgaacgg cgcgggctgt tggagccgcc tctgcggacc
     6241 gaggccggct atcgccagta cggcgcgtcc gacgtcgccc gcatcgagtt cctcctccgc
     6301 gccaaggagc tcggtttcac gctcgccgag atctccgagc tgttcgctgc gaccaccacc
     6361 gacgtcgccg ccgcggtgcg cgccaaactc accgcggtcg aggcgcagat ctccgagctc
     6421 gccgagaccc gctgccgatt gcgccagctc atcgacctgt gcgaccacgg cgacggctcg
     6481 gcctgcctcc aactccgact cgtcggctcc tgacgggccg caccaacgaa cggactcgtg
     6541 acatgaccac gacctctgac ctcacgcgct cgatcgatgt cgacgagctg atcgcgtcga
     6601 ccgagctgcc gctgctgctc gacttcggcg ccgactggtg cccgccgtgc cgcgcgctcg
     6661 acctgatcct cgaccagatc gccgccgaac acatcgactc gatcgtgatc cgcaaggtcg
     6721 atgtcgacca ggtgccggac ctcgtggtgc ggttcggtat cgcctcggcg ccgacgatgc
     6781 tgttgttcgt cgggggtgaa ctcgaggcga agatcgtcgg agcccggccg aaggccgcgc
     6841 tcttggagat gttgggcgac cacctctgag cggagctcag cgaccacctc tgagcggagc
     6901 tcagcgatcg tcctgagccc gcgagcttgt ggcgcggtgc gattggcggg aatactctcg
     6961 gcatgaccga gtcgcctccc gctgagccca ccgatggcgg tctcgccgat gtctctgcgt
     7021 cggcacttcg ggtcttcccc cggcacctgc tcccggccaa gcgcagtgag atcgcccgac
     7081 gcgccacgca gatcgcctgg gtgacggcca agcatttcac gccgctcgcg atccggagcg
     7141 cccgcaagcg cgaggtcgac cccgatgcct tcgcccggcc cctccgcctc acgttcgagg
     7201 agctcgggac cacgttcatg aagttcggcc agctcgtgag ttcgtcgccg ggcgtgttcg
     7261 gcgaggaggt gtcggcagag ttccgttcct gcctcgacac cggtcccgcc gtgccattcg
     7321 agcaggttcg tcgaaccgtc gaggccgagc tcggcatgca tctcgaagag gcgtacgccc
     7381 acttcgatcc cgaacccatc ggtcgcgcct cgatcgccgt ggtgcaccgc gccacgttgc
     7441 acgacggcac cgacgttgcg gtgaaggtgt tgcggcccgg catcgagaag cgggtgtcca
     7501 tcgacatgga cctgttcgag ccgatgctga cgatcatcgc ccggacgaca ggggagtacg
     7561 cggccggcca gctcgtgcag atgttcgacg gtttccgtat gcagctcggc gaggagctcg
     7621 acctgcgcaa cgaggctcgg gcgatccaac acatgcagaa gttgctccgt gtcgtcgagc
     7681 tgccgctcgt cacggtcccc gaggtctacc cggcgttcag cgcagcgcgg gtgctgacga
     7741 tggagttcct cgacggggtt ccggtcgatg acatgtcgcg catcgccgag tacgggttcg
     7801 atccggcgcc ggtcgtgtcc cagaccgtca aggggttcct gatgacgacg gtgcgttggg
     7861 ggttcttcca cggggacgtg cacgccggca acctgctcct cctgcgcgac ggtcgggtcg
     7921 gcgtgatcga ctggggcatc gtcggtcgcc tcgacaagga cacgcacatg ttcttccgtc
     7981 gcatcgtcga ggcagccctc ggcgacgagg acgcgtggag cgatatcgcc gcccacacga
     8041 tcaagcagta cggcgcattg ctcgagaccg gcatgggcat gaaccacgag gacaccaccg
     8101 ccttcatgcg gatgatgctc gagccgctcc tcaaggcgcc cttcggcgag gtgtccctcg
     8161 tcgagatcct caatgcgccg caggcgaagg tcgccgaggc caaggggctc gaggtcgaga
     8221 accggtcgat cgcttcgatc tacaagcggt tcaaggagca gcggaagctg cggttgctcg
     8281 ccgaggccga gggcatctac gagtcggagt tcgagcgagg catgttcctg ctcacgaagg
     8341 cgctcatgta cttcgagcgc tacgggaaga tgtacatgtc cgacgtcggc atcttccacg
     8401 acgaggcgtt cttccgcgcc gcgctcgccg cgcccacgtc gacgctcagg gacgcgccnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca cttagaaagt gacttgtcac
     8581 tttctaagtg gcggtctacg ctcggccggt gcccgcagcc agcaagcgca tcgctcccgt
     8641 tcgccggtcc caggccgagc gtcgcgaaac gacccgcgcc gcactgatgg tcgcagctct
     8701 cgagcagctg ctggtcgacg gcctggccgg cttcacgacc accgaggtgt gcaaacgcgc
     8761 cggcctgagt cagggcgcgc tgttcaagca cttcgacacg aaggccgacc tcctcgcggc
     8821 ggtcaccgag cacctgttcg acgaactgcg cgccgagttc gagtcgacgt tcatcgacct
     8881 gccggccgca cgccggaacc cgcggtcggg gatcgaactg ctgtgggacc agatgctcga
     8941 cgcccgcctc gctgcggcct acgagctcta cacggctgcc cgtaccgaca ccgatctgcg
     9001 ggcggcgctc gaaccggtcg tcgccgcgca cgtcgaacgc atccacgagc tcgctgccgc
     9061 actcattccc ggggtcgacg acgatcgtcg ccggagcgcc gtcgatctgg ccctcctcgc
     9121 gatgcagggt ctcgtcgtgc agcagatggc cctgcccgac ccggcgcagc acgagcggct
     9181 ccgcgaactg ctcgacggcc tgaccgtcgt gttgttgacg aacgagaacg aggggacgaa
     9241 ctgatggacg acctgaagaa cccggcggtt ctggcggtgc cgctctactt cgcgctgatc
     9301 tggtgggagg tgcgggcgct gacccgtctg aaggcggccg gcaaggacgt gctcgggtac
     9361 gagcggcggg acacgtgggc gtcgctcctc atcggggccg tgtcgttgat cacggtcggc
     9421 ggcctgaacc tggtggtcaa gatcttgtcg gagtggctct gggattaccg cctgaccgac
     9481 ctcggcaacg gcatcctcgg ctggacggtc gcgttgttgg gttgggactt cgcgtactac
     9541 tggctccacc gtgtcgagca cgagtcgcgc atcttgtggt cggcccacgt caaccaccat
     9601 tcgagcgagc gctacaacct gtcgaccgcg cttcgtcagc cgtggacgcc ggtcgtgctc
     9661 gtgctgttct tcccgccgct cgcgctcatc ggtgtgcgcc cgtggatgat cgccgtgtcg
     9721 ggcgggttca acctcgtcta ccagttctgg atccacaccg aggcggtcga caagctgccc
     9781 cgctggttcg agttcgtctt caacacgccc tcgcatcacc gggtgcacca cggcagccag
     9841 cagcagtacc tcgatcgcaa ccacggcggg gtgctcatca tctgggaccg gctgttcggc
     9901 acgttcgagc ccgagggcga gcgcgttcgc tacggcctca cgaagaacat caacacgttc
     9961 aacctctgga agatcttcga ccacgagctc gtcgccatcg gacgcgacgt gaaggcggcg
    10021 cctcgttggc gtgaccgctt cggctacgtg ttcttcgggc cgggctgga
//
LOCUS       DAOSOA010000098        10024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6720_length_10024_cov_6.814324, whole genome shotgun sequence.
ACCESSION   DAOSOA010000098 DAOSOA010000000
VERSION     DAOSOA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6720_length_10024_cov_6.814324"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1204
                     /locus_tag="PKY13_08045"
     CDS             233..1204
                     /locus_tag="PKY13_08045"
                     /inference="COORDINATES: protein motif:HMM:NF023015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HOT79923.1"
                     /translation="MTSTISRPVSSTTVDPERAFTTMVGRLSRLSAGKGFDAYRDVDW
                     PEADSPDAVIDPTDPRWILSDVDPFAASEWYRSRSADEQARLGLVRMASCLKTGWHFE
                     NLLQQGLLHRALYLPDGSEEFRYVLHEVIEESQHTLMFNEFVNRSGAPVRGMARWLRL
                     AVEQLVPRVSISDPAFFFVMVLGGEDPIDCLQRKMLANGGGHPLLEQIMRIHIAEEAR
                     HISYARSALKKYVPRLPGWRRHLLSVTVPIVLGIMVRLMVRPTGDLVRAGVPWSVIRD
                     SYRTDAGTELFAECAAKPRVLARELGLMTPLGERVWKRFRIWDADAA"
     gene            1269..>1507
                     /locus_tag="PKY13_08050"
     CDS             1269..>1507
                     /locus_tag="PKY13_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOT79924.1"
                     /translation="MGSIVEFPSNGSTGAGYFAPAAGGGPGVVVIQEWWGLVPHIEEI
                     CDRFAAAGFSALAPDLYHGDSVPNTEPDEAGKLMMA"
     assembly_gap    1508..1607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1608..2672
                     /locus_tag="PKY13_08055"
     CDS             <1608..2672
                     /locus_tag="PKY13_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79925.1"
                     /translation="RRLILDLDADASAADHQLDTQRRHWLRSQTVGLHTVARMLHGDH
                     LDFIDEVELCFGIRPERITEDEVRDAQRELDGALRGAGGGTSAPLAERTAALRDRHVI
                     PVDRLRPVIDNLAQELRERTRTAFGLPDGEHVTFDLVSDKPWSGFNVYEGGLRSLVTI
                     NTDLPVLATSIAHLVAHEAYPGHHTEGCHKEVGLVRERDRIEQTIALIGTPACLLAEG
                     LADLGLEALMGPDTVTAVAPMLADAGVNFEVEPVLAMSHFSEVLARARGTLAIQLHSD
                     GRPVEDVIATAERWFVVDHARATQMVRFLTDPTWRAYVFCYAEGHRLCRSFMGGDPRR
                     FARLLDEQLTPADLIAGAAA"
     gene            2669..4411
                     /locus_tag="PKY13_08060"
     CDS             2669..4411
                     /locus_tag="PKY13_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT79926.1"
                     /translation="MRLLVDRARTFALGGPSGDDDLAAVIDVTATVSPDGQRWSMTIG
                     NPTAVAVPIRRIGWVVPLEVHGPLRIFCNGYQSWSPTGVATLGVDRDPSTSSSFLEVF
                     GGALHADQRQVQGDELRSELFTVLADGSDDIVVAGFSGGDRHDGTFRVRNGAGGPELV
                     IEAFLGGAHLPVGGERVLHDVLVLPGATGEHSALLDRWATDVGRAASARVEAPYQVGW
                     CSWYHYFHKIDEAALDANLALAGDWPFDVFQLDDGYQPAIGDWLRTNDTFDSDLDAIA
                     ARISAAGRRPGIWLAPFSMHPNYEMFGRHPEWLATTTDGAGPIPSMIQDIWDGVAYAL
                     DVTRPEVLDHLEGIARGLHDAGFSYLKLDFTYAPSFDGIYADMTQTPAERVRAAFAAI
                     RRGAGDDAFLLGCGAPLASVLGLVDGMRIGPDVAPNWEPSPDHVGMNGYEETSPSTRN
                     AWQSTLARSFMHRRFWLNDPDCIMLRTEHTDLAPESARAWALAVGASGGMALVSDDLS
                     LLGPAERELLDEVVTLGRMVDDEIRAGAPAPSSPDLMDHRTPTTLVAGRTHIVGTPDA
                     GTAELHHDREQPTP"
     gene            4408..5280
                     /locus_tag="PKY13_08065"
     CDS             4408..5280
                     /locus_tag="PKY13_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79927.1"
                     /translation="MSDATTGMPLPDPIRPPHLVDAGEIDSRLLPVPVRVGAWVELVD
                     GALVGHLRVHPTLTRHGVLEPTALVTMIDMLGGLSADDDIEHWVFTSNMSLRMPPVPA
                     PEALRCTVRTLRAGARSSTSEILINDAGGTPLGMGFVSFSRVPRRPTDLPKPEFDLEI
                     VGEAWSRIEPLDEPPRDAAGMRVVDAATGHLELDLADKVNNPAGALHGAMIGMLLAAA
                     AETLADTTVGGAHVAGDLDIRYLNQARSGPVATRARFVGDPELGSVVVDLVDHGRDDV
                     LVAVATVRTYRVGD"
     gene            complement(5262..5885)
                     /locus_tag="PKY13_08070"
     CDS             complement(5262..5885)
                     /locus_tag="PKY13_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOT79928.1"
                     /translation="MAAATANGGAIPVVRAFDPDDLPPLRVDAPVVLVHGGPSAGAVD
                     GRTIVQLVAARHLGVDPAVIEIDRADRGKPRLGGAPSPFAFNLSDADDHVAAAFGLGL
                     EIGVDIERADRRTVEPERFARRVLSVAEQSAIEGLEGDALDRALIRMWTRKEAVLKAA
                     GHGLRRELADVDTVADVVMLDGSPWNCWTTNWRDLVVSVAWGQSPTR"
     gene            complement(5920..7524)
                     /locus_tag="PKY13_08075"
     CDS             complement(5920..7524)
                     /locus_tag="PKY13_08075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT79929.1"
                     /translation="MIDLTAAASALEAANQVVAAGIARLAEIGLDENQVLAYDVAHAA
                     AAVQTGQSLLGYGIKGDVEASIANAFIADAIAELAGKVFGRESEWGVGFDAFDSTREY
                     VATYRNPEFLAGLAGTAGPRHLSDDFEMVQDAFRRFADEKLKPVAEHIHRHNGDIPED
                     IITGLAEMGAFGLSIAEEYGGFASGTDGDYIGMVVATEELSRGSLGAGGSLITRPEIL
                     ARALEAGGTEEQKHEWLPKLASAEVMNAVAVTEPDFGSDVAGVTVSATATTEDGVEGY
                     VINGVKTWCTFGARADVLMVLARTDPDRSIGHRGLSMFIVPKLRGEGHGFVVHQNDME
                     GPGAGKMEGRPIDTIGYRGMHSYEIALEDWFVPATNLIGLDGGKGKGFYYQMAGFENG
                     RLQTAARAVGVMQAAYESALAYAGDRKVFGKAVGDYQLTQAKLARMAVIIQASRQFSY
                     DVARLMAKGEGQLEASLVKAYVCKAAEWVTREAMQIHGGMGYAEEYDVSRYFVDARVL
                     SIFEGADETLCLKVIARRLVENHQKG"
     gene            complement(7535..>10024)
                     /locus_tag="PKY13_08080"
     CDS             complement(7535..>10024)
                     /locus_tag="PKY13_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT79930.1"
                     /translation="IIAIAPMFGPHRLWFATVFAAATAVQNLALGRIVAATGRMPWGM
                     AAFDHALACAAGLLIPETLIWAIMVNAIGLPVTVISHGRRFALGLLAVSVPAYVTVGL
                     VTGADLWPIAVGVFVVAGLGNCGLVGDAAEEERSVRARYSDILDQLQVVVFESPGIGQ
                     PLTYVNDHVGKMLGFTRTEWLEPAWWESRLHPDDHEAWVESNVQSIAGVSHQATYRMY
                     AEDGRVVHILEITRVLEVGSGRTKIRGVLVDVSRQALAEAEASQLSMFVDQIPLALQI
                     LEITDPDDVAAIRMVAANRVACENVDATLEELVHEWPRRYAVVDSQPGNLEAFAEVRR
                     TGTSLTKHERSWVDRDGRDRRVNIEAFALGGDYVGVSYEDITERANAEKALRHQANHD
                     ALTGLPSRAFLAQRLDAALTACADGADGACQGALLMMDLNQFKEVNDSLGHHHGDRLL
                     VELSGRLSAIAGDEHVIARLGGDEFAMIVEGGTPRQALELAAHIGRRFAEPIVIDGVT
                     LQTNVSVGIALYPEHASDADGLMQRADAAMYRAKTGGTGVSLFTPEQSTAGIRRLQLL
                     SDLRTAVGNSELMLHYQPRVLFGSGEIEGLEALVRWVHPTWGMLPPDEFISLAEVSGL
                     IQELTQFVIRVAVADAAHLRSVGHDLPVAVNLSVRNLYSPDLVDGILKALAAHDLPSS
                     SLRVELTESEIMDDPSVAMQVLQRLRSEGVEVSIDDFGTGYSSLSYLRDLPIDEIKID
                     RSFVADITDGDDVVVRSIIDLGHALGVSVVGEGVETVQQWDHLHRLGCDVAQGYLISR
                     PLPFEELQRFLDDRRDFSASPTTLGAPVTRG"
BASE COUNT         1469 a         3565 c         3363 g         1527 t
ORIGIN      
        1 ccgtcgagcg gcgtcgactt gagcaggtgc agtccggcgt cgaccagctg ggcgcgccgg
       61 tcgtcggggt tcaggcggcg ctgggtcggg gcgctcggcg cagcagcggg gtcggagggc
      121 acgtcgggag cgtacccctc gcccgacgtg ggtggacggt tgactctgac actgttgacc
      181 ggcggtcagt tcgttgccta ctgtgctgac ctcaggtcaa caggaggttc gcatgaccag
      241 cacgatcagc cgccccgtca gcagtacgac cgtcgatccc gaacgcgcct tcaccaccat
      301 ggtcggtcgg ctcagccgtc tgtccgccgg caagggcttc gacgcctacc gcgacgtcga
      361 ctggcccgag gccgactcgc ccgacgcggt catcgatccg accgaccccc gctggatcct
      421 gtccgacgtc gatcccttcg ccgccagcga gtggtaccga tcacgatcag ccgacgaaca
      481 ggcccggctc ggcctcgtcc gaatggcgtc gtgcctgaag accgggtggc acttcgagaa
      541 cctcctccag cagggcctcc tccaccgggc gctctacctg cccgacggca gcgaggagtt
      601 ccgctacgtc ctccacgagg tcatcgagga gtcccagcac accctcatgt tcaacgagtt
      661 cgtcaaccgg tcgggcgcgc cggtgcgggg catggcccga tggctccgac tcgcggtcga
      721 gcagctcgtt ccccgggtct cgatcagcga ccccgccttc ttcttcgtga tggtgctggg
      781 cggagaggat ccgatcgact gcctgcagcg caagatgctc gccaacggcg gtgggcaccc
      841 gctgctcgag cagatcatgc gcatccacat cgccgaggag gcccgccaca tctcctacgc
      901 ccgctcggcg ctcaagaagt acgtgcctcg cctgccgggg tggcggcggc acctgctgtc
      961 ggtcaccgtt ccgatcgtgc tcggcatcat ggtgcggctg atggttcgac cgaccggcga
     1021 cctggtccgt gccggcgtgc cgtggtcggt catccgcgac tcgtaccgca ccgacgccgg
     1081 caccgagctg ttcgccgagt gcgcggcgaa gcctcgcgtc ctcgcccgcg agctcgggtt
     1141 gatgaccccg ctcggcgaac gggtgtggaa gcgcttccgc atctgggacg ccgacgccgc
     1201 ctgacgccga cgccgcacgg ccctgacgcc cgcggctggt cccgaatcgg tcgggctatg
     1261 gtctgctcat gggcagcatc gtcgagtttc cgagcaacgg ttccactgga gcgggctact
     1321 tcgcccctgc ggccggaggc ggtccgggcg tcgtcgtcat ccaggagtgg tggggtctcg
     1381 tcccccacat cgaggagatc tgtgaccggt tcgcggctgc cggcttcagc gctcttgccc
     1441 ccgatctcta ccacggtgac tcggtgccga acaccgaacc cgacgaggcc ggcaagctca
     1501 tgatggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc cgcctcatcc
     1621 tcgacctcga tgccgacgcc agcgcggccg accaccagct cgacacccag cgtcgacatt
     1681 ggctccggtc ccagacggtc ggtctgcaca cggtcgcccg catgctgcac ggcgaccacc
     1741 tcgacttcat cgacgaggtc gagctgtgct tcgggatccg tcccgaacgc atcaccgagg
     1801 acgaggtccg cgacgcgcag cgtgagctcg acggagcgct gcggggcgcc ggcggaggca
     1861 cctcggcacc gctcgccgaa cgcaccgcgg cgttgcgtga tcgccacgtc atccccgtcg
     1921 atcggctgcg gccggtcatc gacaacctgg cgcaggagct gcgggaacgc actcgcaccg
     1981 cgttcggcct gcccgacggc gaacacgtca cgttcgatct cgtgagcgac aagccctggt
     2041 cgggcttcaa cgtctacgag ggcggtctcc gcagcctcgt gacgatcaac accgaccttc
     2101 cggtgctggc cacctcgatc gcccacctcg tcgcacacga ggcctacccg ggtcatcaca
     2161 ccgaggggtg tcacaaggag gtcggcctcg tacgcgaacg cgatcgcatc gagcagacga
     2221 tcgcgctcat cggcaccccg gcctgcctac tcgccgaagg cctcgccgac ctgggcctcg
     2281 aggcgctcat gggccccgac accgtgacgg cggtcgcgcc gatgctggcg gatgccggcg
     2341 tcaacttcga ggtcgagccg gtgctggcca tgagccactt ctccgaggtc ctcgcccggg
     2401 cgcggggaac gttggccatc cagctccact ccgacggccg ccccgtcgag gacgtgatcg
     2461 cgacggccga gcgctggttc gtcgtcgatc acgcccgggc cacccagatg gtgcgtttcc
     2521 tcaccgaccc cacctggcgg gcgtacgtgt tctgctacgc ggagggccac cggctctgcc
     2581 gctccttcat gggcggcgat ccccgccggt tcgcccgcct gctcgacgag cagctgaccc
     2641 ccgcggatct gatcgccggc gccgcggcat gaggctcctg gtcgaccgcg cccgcacgtt
     2701 cgcgctcggt gggccctccg gggacgacga cctcgcagcg gtcatcgacg tcacggccac
     2761 cgtcagcccc gacggccaac ggtggtcgat gacgatcggc aacccgaccg cggtcgccgt
     2821 cccgatccgg cgcatcggct gggtggtacc gctcgaggtg catggtccgc tccgcatctt
     2881 ctgcaacggc taccagtcat ggtcgccgac gggcgtcgct accttgggcg tcgaccggga
     2941 cccgtcgacg tcgtcgtcct tcctcgaggt cttcggcgga gcgttgcacg ccgaccagcg
     3001 ccaagtgcag ggcgacgagc tccggtcgga gctgttcacg gtgctcgccg acggcagcga
     3061 cgacatcgtt gtcgcggggt tctccggcgg tgaccgccac gacggcacgt tccgggtccg
     3121 caacggcgcc ggcggtcccg agctggtgat cgaggcgttc ctcggcggag cgcatctccc
     3181 ggtcggcggt gagcgggtgc tgcacgacgt gctcgtgctc ccgggcgcga cgggtgagca
     3241 cagcgcgctg ctcgaccgat gggccaccga cgtcggtcga gctgcgagcg cccgagtgga
     3301 ggcgccgtac caagtggggt ggtgctcctg gtaccactac ttccacaaga tcgacgaggc
     3361 cgcgctcgac gccaacctcg cgctcgccgg agactggccc ttcgacgtct tccagctcga
     3421 cgacggctac cagcccgcca tcggcgattg gctgcgtacc aacgacacct tcgattcgga
     3481 cctcgacgcg atcgcggccc ggatctccgc cgccggccgc cggcccggca tctggctcgc
     3541 cccgttctcg atgcacccga actacgagat gttcggccgt catcccgagt ggctggcgac
     3601 caccaccgat ggtgccggcc ccatcccgtc gatgatccag gacatctggg acggggtcgc
     3661 ctacgcactc gacgtcaccc ggcccgaggt gctcgaccac ctcgagggca tcgcccgggg
     3721 actccacgac gccggattct cctacctcaa actggacttc acctacgcgc cgagcttcga
     3781 cgggatctac gccgacatga cgcagacccc ggccgagcgg gtgcgggcgg ccttcgctgc
     3841 gatccgtcgt ggtgccggcg acgacgcctt cctccttggc tgcggtgccc cgctcgccag
     3901 cgtcctcggc ctggtcgacg gcatgcgcat cggtcccgac gtcgcgccga actgggagcc
     3961 gtcgcccgat catgtcggga tgaacggcta cgaggagacg tcgccgtcca cccgcaacgc
     4021 gtggcagtcg accctggccc ggtccttcat gcaccgccgc ttctggctca acgaccccga
     4081 ttgcatcatg ctgcgcaccg agcacaccga cctggcgccc gaatcggcac gcgcgtgggc
     4141 gctcgccgtc ggggcctccg gtggcatggc gttggtgtcc gatgatctgt cgttgctcgg
     4201 tccggcggag cgcgagctgc tcgacgaggt cgtcacgctc ggccgaatgg tcgacgacga
     4261 gatccgcgcc ggcgccccgg caccctcgag ccccgacctg atggaccatc gcaccccgac
     4321 gaccctcgtc gccggtcgga cccacatcgt cggcacaccc gacgccggca cggccgaact
     4381 gcaccacgac cgagagcaac cgacaccatg agcgacgcca ctaccggcat gcccctcccc
     4441 gacccgatcc gaccgccgca cctcgtcgac gccggcgaga tcgactcgcg cctcctgccc
     4501 gtccccgttc gggtcggcgc ctgggtcgaa ctcgtcgacg gggcgctcgt cggccacctg
     4561 cgcgtgcatc ccaccctcac ccggcacggg gtcctcgaac cgacggcact cgtgacgatg
     4621 atcgacatgc tcgggggcct gtccgccgac gacgacatcg aacactgggt gttcacgtcg
     4681 aacatgtcgc tccgaatgcc accggttccg gcgcccgagg cgctccgctg caccgtgaga
     4741 acacttcgcg ccggtgcccg gtcgtccacg tcggagatcc tcatcaacga cgccggcgga
     4801 actccgctcg gcatggggtt cgtgtccttc tcacgggtgc cgcgccgccc caccgacctc
     4861 cccaagcccg agttcgacct cgagatcgtg ggcgaagcct ggtcgaggat cgagccgctc
     4921 gacgagccac cccgcgatgc cgccggcatg cgggtggtgg acgctgcgac cggccacctc
     4981 gagctcgacc tcgccgacaa ggtcaacaat ccggccggcg ccctgcacgg tgccatgatc
     5041 ggcatgctgc tcgccgctgc cgccgagacc ctcgccgaca cgaccgtcgg gggcgctcat
     5101 gtcgccggcg acctcgacat ccggtacctc aaccaggccc gcagcgggcc ggtggccacc
     5161 cgggcgcgct tcgtcggcga cccggagctc ggttcggtcg tggtcgacct cgtcgaccac
     5221 ggccgcgacg acgttctcgt cgccgtggcg accgtgcgca cctaccgggt cggcgactga
     5281 ccccacgcga ccgacaccac gaggtcgcgc cagttcgtcg tccagcagtt ccacgggctc
     5341 ccgtcgagca tcaccacgtc ggccaccgtg tcgacgtcgg cgagctcgcg ccgcagacca
     5401 tggccggccg ccttcaacac ggcctccttg cgggtccaca tgcggatgag ggcccggtcc
     5461 aacgcgtcgc cctcgaggcc ctcgatcgcc gactgttcgg cgacgctcaa cacccggcgg
     5521 gcgaagcgct cgggttccac cgtgcgacgg tcggcccgtt cgatgtcgac gccgatctcg
     5581 agccccaacc cgaaggccgc ggcgacgtgg tcgtcggcgt cgctcaggtt gaacgcgaag
     5641 ggcgaggggg cgccaccgag gcggggtttg ccgcggtccg cccggtcgat ctcgatgacg
     5701 gccggatcca cgccgaggtg ccgcgccgcg accagctgca cgatcgtgcg gccgtcgacc
     5761 gctccggccg acggcccgcc gtgcacgagc acgaccgggg catcgacgcg gagcggcggt
     5821 aggtcgtcgg gatcgaaggc gcggacgacg gggatcgctc caccgttcgc ggtggcggcg
     5881 gccaccggcg gtgcgctccg ctgcgctccg gcctctccgc tagcccttct ggtggttctc
     5941 gacgaggcgg cgggcgatga ccttgaggca caaggtctca tccgcgccct cgaagatcga
     6001 cagcacgcgg gcgtcgacga agtagcggct cacgtcgtac tcctcggcgt agcccatgcc
     6061 gccgtggatc tgcatggcct cgcgggtgac ccactcggcc gccttgcaca cgtacgcctt
     6121 gacgagcgac gcctcgagct ggccctcgcc cttggccatg aggcgggcga cgtcgtagga
     6181 gaactggcgg gacgcctgga tgatgaccgc catgcgagcg agcttggcct gcgtgagctg
     6241 gtagtcgccg acggccttgc cgaacacctt gcggtcgccg gcgtacgcga gggccgactc
     6301 gtacgcggcc tgcatgacgc cgacggcgcg ggccgcggtc tggagccggc cgttctcgaa
     6361 cccggccatc tggtagtaga agcccttgcc cttgccaccg tcgagaccga tcaggttggt
     6421 ggccggcacg aaccagtctt ccagcgcgat ctcgtaggag tgcatgccgc ggtagccgat
     6481 ggtgtcgatc gggcgaccct ccatctttcc ggcgccgggg ccctccatgt cgttctggtg
     6541 caccacgaag ccgtgaccct cgccgcgcag cttggggacg atgaacatcg acaggccgcg
     6601 gtggccgatc gaacggtcgg ggtcggtacg ggcgagcacc atcaggacat cagcgcgggc
     6661 accgaacgtg caccacgtct tgacgccgtt gatgacgtag ccctcgacgc cgtcctcggt
     6721 cgtcgcagtg gccgacacgg tgacgccggc gacgtcggat ccgaagtcgg gctcggtcac
     6781 ggcgacggcg ttcatcacct cggccgacgc gagcttggga agccactcgt gcttctgctc
     6841 ctcggtgccg ccggcctcga gggcccgggc gaggatctcg gggcgggtga tcagcgaacc
     6901 gccggcaccg agggagccac gcgagagttc ctcggtcgcg acgaccatgc cgatgtagtc
     6961 gccgtcggtg ccggaggcga agccgccgta ctcctcggcg atgctgaggc cgaaggcgcc
     7021 catctcggcg agccccgtga tgatgtcctc ggggatgtcg ccgttgtggc ggtggatgtg
     7081 ctcggcgacc ggcttgagct tctcatcggc gaagcggcgg aaggcatcct gcaccatctc
     7141 gaagtcgtcg ctcaggtgac gaggcccggc agtgcccgcc aacccggcga ggaactcggg
     7201 gttgcggtag gtcgccacgt actcacgggt cgagtcgaag gcgtcgaagc cgacacccca
     7261 ctccgactca cgaccgaaca ccttgccggc cagctcggcg atggcgtcgg cgatgaaggc
     7321 gttcgcgatc gaggcctcga cgtcgccctt gatgccgtag cccaacaggc tctggccggt
     7381 ctgcactgcg gcagccgcgt gggccacgtc gtaggcgagg acctggttct cgtcgaggcc
     7441 gatctcggcg agccgggcga tgccggcggc cacgacctgg ttggcggcct cgagggcgct
     7501 tgcagcagcg gtcagatcaa tcatggagcc gaggttaccc gcgggtaacc ggagctccca
     7561 aggtggtcgg ggaggcgctg aagtcgcggc gatcgtcgag gaaacgctgc aactcctcga
     7621 acgggagcgg acgactgatc aggtacccct gggcaacgtc gcagccgagc cggtggaggt
     7681 ggtcccactg ctgcacggtc tccaccccct cgcccacgac cgacacgccc agcgcgtgac
     7741 cgaggtcgat gatcgaacgc actacgacgt cgtcgccgtc ggtgatgtcg gcgacgaagg
     7801 aacgatcgat cttgatctcg tcgatcggca ggtcgcggag gtacgacagc gacgagtagc
     7861 ccgtgccgaa gtcgtcgatg gacacctcga cgccttccga ccggagccgc tgcagcacct
     7921 gcatcgcgac cgaggggtcg tccatgatct cgctctcggt gagttcgaca cgcaacgaac
     7981 tcgacggcag gtcgtgcgca gcgagcgctt tcaggatgcc gtcgacgagg tcgggggagt
     8041 agaggttgcg gaccgacagg ttgacggcga cgggcaggtc gtggcccacg ctgcggaggt
     8101 gggcggcgtc ggcgacggcg acgcggatga cgaactgggt cagctcctgg atcaggcccg
     8161 acacctcggc gagcgagatg aactcgtcgg gcgggagcat gccccacgtc gggtgcaccc
     8221 agcgcaccag cgcctcgagg ccctcgatct ctccggagcc gaagagcacc cggggctggt
     8281 agtgcagcat cagctcactg ttcccgaccg cggtgcgcag gtcgctcagc agctgcagcc
     8341 gccggatccc ggcggtcgac tgctcggggg tgaacagcga gacaccggtg ccgcccgtct
     8401 tggcgcggta catcgcggcg tcggcgcgtt gcatcagccc gtcggcatcg gacgcgtgct
     8461 cggggtacag cgcgatgccg acgctcacgt tggtctggag cgtgaccccg tcgatcacga
     8521 tcggctcggc gaacctccgc ccgatgtgcg ccgcgagctc gagggcctgt cgcggcgtgc
     8581 cgccctcgac gatcatggcg aactcgtcgc cgccgagccg ggcgatcacg tgctcgtcgc
     8641 ccgcgatggc gctcaggcga ccgctcaact cgacgagcag ccggtcgccg tggtgatgac
     8701 cgagcgagtc gttgacctcc ttgaactggt tgaggtccat catcagcagc gctccctggc
     8761 aggcgccgtc cgcgccgtcg gcgcacgccg tcagcgcagc gtcgagccgc tgggcgagga
     8821 acgcccggct gggcaggccc gtgagagcgt cgtggttcgc ctggtgccga agcgccttct
     8881 cggcgttcgc ccgctcggtg atgtcctcgt aggagacccc gacgtagtcg ccaccgaggg
     8941 cgaacgcctc gatgttgacg cggcggtcac ggccgtcgcg gtcgacccac gaccgctcgt
     9001 gcttggtgag cgacgttccg gtccgacgga cctcggcgaa ggcctccagg ttcccgggct
     9061 ggctgtccac cactgcgtag cgacggggcc actcgtgcac gagttcctcg agggtcgcgt
     9121 cgacgttctc gcacgcgacg cggttcgccg cgaccatgcg gatcgctgcg acatcgtcgg
     9181 ggtcggtgat ctcgaggatc tgcagtgcca gcgggatctg gtccacgaac atgctgagct
     9241 ggctggcctc ggcctcggcg agcgcctgtc ggctcacgtc gacgaggacc ccacggatct
     9301 tcgtacgacc cgaacccacc tcgagcacac gggtgatctc gaggatgtgc accacccgac
     9361 cgtcctcggc gtacatgcgg taggtcgcct ggtgggagac cccggcgatc gactgcacgt
     9421 tcgattcgac ccatgcttcg tggtcgtcag ggtgcagcct cgactcccac cacgccggct
     9481 cgagccactc ggtgcgggtg aagccgagca tcttgccgac gtggtcgttg acgtacgtca
     9541 acggttgccc gattcccggc gactcgaaga cgacgacctg cagctggtcg aggatgtcgc
     9601 tgtaccgggc gcggacgctg cgctcctcct cggcggcgtc gccgacgagg ccacagttgc
     9661 cgagcccggc gaccacgaac accccgaccg cgatcggcca gagatcggcg cccgtgacga
     9721 gcccgaccgt gacgtacgcc gggaccgaca ccgcgaggag gccgagggcg aagcgtcgac
     9781 cgtgcgagat caccgtgacc ggcagtccga ttgcgttgac catgatcgcc cagatcaggg
     9841 tctcggggat caggagtccg gccgcgcagg cgagtgcgtg gtcgaacgcg gccatgcccc
     9901 acggcatgcg cccggtggcg gcgacgatgc gcccgagcgc gaggttctgg accgcggtgg
     9961 ctgcggcgaa caccgtcgcg aaccacaggc ggtgcgggcc gaacatcgga gcgatcgcga
    10021 tgat
//
LOCUS       DAOSOA010000099         9944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6773_length_9944_cov_5.656588, whole genome shotgun sequence.
ACCESSION   DAOSOA010000099 DAOSOA010000000
VERSION     DAOSOA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6773_length_9944_cov_5.656588"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1265
                     /locus_tag="PKY13_08085"
     CDS             180..1265
                     /locus_tag="PKY13_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79931.1"
                     /translation="MNLPDEARSPDDEPPADHPTVILWNGFDPDIGHRARMPIFCGVP
                     MQLMDDVMYIATARSRPAARLLARTPDLMRNLSHRTTVKTERCVGHVRGPIQWSETIT
                     AWASGVGVNDVFVCGTPRRDFDVPENRLIVWLLKRLVSAGRRTAGDAAAWFDPDEIER
                     VRLQGRTAQQLLSHPSLRGISSRKLDGREWRLVRKSRHLDTYGPAIKLADRVAKPFGA
                     NEARAIASAATQEHHRVMVMLLEAVRSRGYAVPQLTIRGDFLESGHLRYRNPYIMVDR
                     RLSAESGLFFGSARVLTRADTVPEDDRGTAVLVITPADAAGVVDAVLGEPLPAPVVAP
                     VPTPPSAPSGAQSSASGLVQLPGSYSS"
     gene            complement(1214..1387)
                     /locus_tag="PKY13_08090"
     CDS             complement(1214..1387)
                     /locus_tag="PKY13_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79932.1"
                     /translation="MKKGRHRRFEEARALKRGPDIDIEGILNDGAPTADVEDDDDYEE
                     YEPGSWTNPEADD"
     gene            complement(1550..2737)
                     /locus_tag="PKY13_08095"
     CDS             complement(1550..2737)
                     /locus_tag="PKY13_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOT79933.1"
                     /translation="MASPAAPPPAPPHDLITTASALDALVEEVCSADAYAIDTEFHRE
                     RTYFPQLALIQIEVNDVIHLVDPLAIDPTAMRPMFESDALAILHAARQDLEVLEHATG
                     APPRRVFDTQLAAGFLGYSSPSLSSLLEGELGVRAPKADRLTDWLRRPLTDRQLVYAA
                     ADVANLRALHAKQTAELEQRGRIGWVAEATAEMVAEPRGPRDPSEAWRRIKEVRHLRG
                     ADLAVAQELAAWRERRAQELDLTPRYVLADLALVGLAVARPTQLDDLKDIRGVDQRSL
                     RHVADDLFATIAGAAENKPRRDAGSVVSELPAAMRPAVPLVTAWVAQLSRELSIDPAI
                     IATRSDLEAFLRDDPDARLGTGWRAELVGIPLRRLVSGEVAMAFDGQRGLVIEERSGI
                     PAG"
     gene            complement(2730..3056)
                     /locus_tag="PKY13_08100"
     CDS             complement(2730..3056)
                     /locus_tag="PKY13_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT79934.1"
                     /translation="MTEPREIDVDELAERLAEGVVVIDVREADEYDSGHVPGALFVPL
                     SELEQRVDEVPSGEPVLVICKSGGRSMRACNFLAPLGRDVTNIAGGTMAWIDSGRDVV
                     TGMERG"
     gene            complement(3061..3639)
                     /locus_tag="PKY13_08105"
     CDS             complement(3061..3639)
                     /locus_tag="PKY13_08105"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOT79935.1"
                     /translation="MTGAPRPTTIGAVLVGGESSRMGADKASLDVAGTPLGARSVRAL
                     EAAGLTDIMLVGATSAHGGLGGRVVEDLWPGEGPVGAVLTALHAAFLVGATHVVCLAC
                     DLPAVTADAVRPLLDVVEARQAVLVAVDGRPAPPNGVWPVALLPALEDRFGDGASSFR
                     ELLDGIDVVEVDGGAAFADADEPGDLAPFQVG"
     gene            complement(3653..4882)
                     /locus_tag="PKY13_08110"
     CDS             complement(3653..4882)
                     /locus_tag="PKY13_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT79936.1"
                     /translation="MGDSTFPITGRSSADLLTELAEMRPRDIDWRHGKAFSLVYNADE
                     PELEELLHTVANDFLHENALNPFRYETLLKMETEVIDYAKSLFGASAGSLSSGGTESI
                     FLAVYTAREWALQRGITHPTLVCTDTAHPAFAKACHYLGVEMVRLDHGLDGRAVPALY
                     EEAIDERTAILVASTPCYPYGVIDPVTEIAAIAAENELLCHVDACLGGWLLPWWERLG
                     EPVPPWDFRVEGVTSLSADIHKYGYVFKGVSTITYRDRELYQHQIFMYDSWPGGLYAS
                     STAAGTRPGPPIAGAWAAINHLGEEGYLRLATRVLDATRRMQAGIRAIDGLHITSDPD
                     MSVFEFGSDRHDMDAVCDEMDDRGWNLDRQQGGLHLMLAPGHDRVVDAFLADLAESVA
                     SHGPARGGEHVYGGVVE"
     gene            complement(4913..>5136)
                     /locus_tag="PKY13_08115"
     CDS             complement(4913..>5136)
                     /locus_tag="PKY13_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HOT79937.1"
                     /translation="GYHRGLDALRGNGWRGSGYVRWRHESNRGVLAAINGLLSAATEI
                     GEQDEIERLSIFLRQLDPEWDSHGGGQLR"
     assembly_gap    5137..5236
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5237..5499)
                     /locus_tag="PKY13_08120"
     CDS             complement(<5237..5499)
                     /locus_tag="PKY13_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HOT79938.1"
                     /translation="MSDINLSPSGPPETVLPPEPAALSAQLAAAGSDRDAVAAVVVAY
                     PRSSTAWAALGALGRDPVERYAAYRVGYHRGLDALRGNGWRGSG"
     gene            complement(5509..6591)
                     /locus_tag="PKY13_08125"
     CDS             complement(5509..6591)
                     /locus_tag="PKY13_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT79939.1"
                     /translation="MARVVRPLVRRRPRTAVVLGGGGNLGAIQVGQLRALAERGIVPD
                     IVLGCSVGALNGGAFAVDPSMAGVRHLEQIWTEIQHEPETLMPGSWIPNPVQLLRKGS
                     ALNPNDGLSRTIRRFLSGRERFDELTVPFQCVATDVDAGAETWFSEGPLLEPILASAA
                     LPAVYPVVTIGERRFLDGGVLNNVPIARAIELGARKIYVLHVGLHGKPKPAITRPADA
                     ALIAYWIARNGRFSRDLASLPRNVEAIVLPPGERPELRFDDFDHTDELMEQGYLNAVG
                     YLDQLEADDEDEAMGDRAERLRADARRVIDEMRGKVTGRWGHGQPTERDAALERDELD
                     PDDADVPSELDGGSPDDADVDVTPLT"
     gene            complement(6603..7514)
                     /locus_tag="PKY13_08130"
     CDS             complement(6603..7514)
                     /locus_tag="PKY13_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT79940.1"
                     /translation="MSESVRDVVRRPAPEAITIAGLAADHYRPAGEPDGPPLPDGDGR
                     LRSGSLLRPSGAASASAPPLVIVHGAMDRAAGFRRTARHLPFRDIVAYDRRGYAGSRD
                     VPPVREFATQVDDLVAVLTELIAANGERRLVVGHSAGGLIALHALARPGIEELAVGAV
                     VWEAPLAWNDWYTSRGSGLLDLAPDVAAEAFMRGVLGDRLWERLPATMQAERRAEGRA
                     LQADMFNARSPEAAVDLSLITLPVVIGLGTESSEHHRRSATEAAAGIESCRLVAIEGA
                     DHGVHLSGPTAFAELIESWPSGPESPR"
     gene            complement(7511..8941)
                     /gene="pyk"
                     /locus_tag="PKY13_08135"
     CDS             complement(7511..8941)
                     /gene="pyk"
                     /locus_tag="PKY13_08135"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOT79941.1"
                     /translation="MPRRTKIIATVGPASDDPVVIRQMIEAGVDVFRIGLAHGSLSDA
                     VERYRTIRQIAADANRVVGILADLPGPKVRLGQFDEGGVALVPGSTVVLRPGDPTSSA
                     SSLGVDYTDLLVDLQPGDSLAVGDGTVRLTVSSVDRTSAKAEVTYGGLVQGRPGLHIP
                     SDRLGLATPTAEDLYKLDAFVEEGVDMIAVSFVRSAHDIRRVGTEPYPRGPLVIAKIE
                     TRAAVENLEGIIEASGAVMVARGDLGIEMGIEELPHLQKSIIRECIAYGRPVITATQM
                     LESMITAATPTRAEVSDVANAIFDGSSAVMLSAESAIGASPVNSVATMARICERADAE
                     FDYEGWARRLRALRSIHVSDDETRLTDAMTGAGWRAATEMGASAILCISESGFTVRSI
                     ARFRPSMPILGFSPNPRTERQLTLSWGTTPLIAPDISEGDLMNGLVVAARDAGHIRSG
                     DYVAVLAGARQNRTRATDQLRLVRVP"
     gene            9277..9708
                     /locus_tag="PKY13_08140"
     CDS             9277..9708
                     /locus_tag="PKY13_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79942.1"
                     /translation="METSTPTSRSARRRRRIAVAVIAGVGTFGVIGASAASLGGITGS
                     SLGADAAVIGSCDTDGVSLTYTNTYDATLGRYQTTGVNVTGIAAACAGKALALTLKDT
                     GGVSLGSGTVASIVGTSSSVTLTAPGANANAVVGAAVVISG"
     gene            9708..>9944
                     /locus_tag="PKY13_08145"
     CDS             9708..>9944
                     /locus_tag="PKY13_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79943.1"
                     /translation="MSRRARRPSLGIVGLVVGAQLMVSGFGVASAASLGGLTTARLTA
                     NTVAGTSGAPTVVAWENFNGTTGTNLGGTTTDGGA"
BASE COUNT         1508 a         3468 c         3455 g         1413 t
ORIGIN      
        1 tgcgatcctt gatctgatcg agcgaatgcg gctccagctc gacggcatcg acgagagtcg
       61 cggcccggcg ctgttccggg cgatcaccga gcacctcgac ggcccggaga tcgccgaggt
      121 ccagcgctgc atgtccgaca tcttgggttt cgaacccgtc ggctgagcgc cggcccgtca
      181 tgaacctccc ggacgaggcc aggtcgcccg acgacgaacc gcccgccgat cacccgacgg
      241 tgatcctctg gaacggcttc gatcctgaca tcggccaccg cgcccgcatg ccgatcttct
      301 gcggcgtgcc catgcagctc atggacgacg tcatgtacat cgccacggcg cggtcccggc
      361 cggcggcccg cctgctcgca cgcacccccg acctgatgcg caacctgtca caccgcacga
      421 cggtgaagac cgagcgttgc gtcggccacg tccgcggccc catccagtgg agcgaaacga
      481 tcaccgcgtg ggcatcgggt gtgggggtga acgacgtgtt cgtctgcggc acgccgcgac
      541 gcgacttcga cgtgccggag aaccgcctca tcgtgtggtt gctgaagcgc ctcgtctcgg
      601 ccggccgccg caccgccggt gacgccgccg cctggttcga ccccgacgag atcgagcggg
      661 ttcggctcca gggccgcacc gcccaacaac tcctctccca tccgagcctg cggggcatct
      721 cgtcgcgcaa gctcgacggc cgggaatggc ggctcgtgcg caagagccgc cacctcgaca
      781 cctacggtcc ggccatcaaa ctggccgacc gggtggccaa acccttcgga gccaacgagg
      841 cccgggcgat cgcatcggcg gcgacccagg agcaccaccg ggtgatggtg atgctgctcg
      901 aggcggtcag gtcacgcggc tacgcggtgc cccagctcac gattcgcggc gacttcttgg
      961 agtcgggcca cctgcgctac cggaacccct acatcatggt ggaccgccgg ctgagcgccg
     1021 agtccggtct gttcttcggc tcggcccggg tcctcacccg cgccgacacc gtcccggagg
     1081 acgatcgagg gaccgctgtg ctcgtgatca cccccgccga tgcggcagga gtggtcgacg
     1141 ccgtgctcgg ggagccgctg ccggcaccgg tggtggcacc ggttccgacg ccgccctcag
     1201 ctccctcagg agctcagtcg tcggcctcgg ggttggtcca actgcccggc tcgtactctt
     1261 cgtagtcgtc gtcgtcttcg acgtcggcgg tcggagcgcc gtcgttcagg atgccctcga
     1321 tgtcgatgtc cggaccacgc ttcagcgcac gtgcttcctc gaatcgacga tgacgaccct
     1381 tcttcacggc tgtctcccaa tcactgacga acgactcaac gtgccatcct aacgtcggca
     1441 tgaaggcccg caccaaagcg ggtggtcact ttctcacgcc caccggccaa ccaccggtca
     1501 ggttccggaa ttccgctccg gaccgccgat gtgcacgatt tcgggcgcct cagccggcgg
     1561 gaattccgct tcgctcttcg atcaccaacc cgcgctgacc gtcgaatgcc atggcgacct
     1621 cgccgctcac gaggcggcgc agggggatgc ccaccagctc agctcgccac ccggtcccca
     1681 gacgggcatc ggggtcgtcg cggaggaagg cctcgaggtc ggaacgcgtc gcgatgatcg
     1741 ccggatcgat cgacagctcc cgggacagct gggcgaccca ggccgtgacg agcggaacgg
     1801 ccggacgcat cgccgcaggc agctcgctga ccaccgaccc ggcatcgcga cgaggcttgt
     1861 tctccgccgc accggcaatg gtggcgaaca ggtcgtcggc cacatggcgg agcgaacgct
     1921 ggtcgacgcc ccggatgtcc ttgaggtcgt ccaactgcgt cgggcgggcg acggcgagcc
     1981 cgaccagcgc gagatcggcg agcacgtagc gcggcgtgag gtcgagctcc tgggcccgac
     2041 gctcgcgcca cgctgccagc tcctgggcca cggcgagatc ggcaccgcgg aggtggcgca
     2101 cctccttgat gcgacgccac gcctcggacg gatcgcgggg cccgcggggc tcagcgacca
     2161 tctccgcggt cgcctcggcg acccagccga tccgaccgcg ctgctccagc tcggcggtct
     2221 gcttcgcgtg gagcgctcgg aggttcgcta catcagcagc ggcatagacg agctgccggt
     2281 cggtgagcgg ccggcgcagc cagtcggtga ggcggtcggc cttcggcgcc cggacgccga
     2341 gctcgccctc gaggagcgag gacaaggagg ggctcgagta accgaggaac cccgcggcga
     2401 gctgggtgtc gaacacccgg cgcggcgggg cgccggtggc gtgttcgagc acttcgaggt
     2461 cctggcgagc ggcatgcagg attgccaggg cgtcggactc gaacatgggg cgcatcgccg
     2521 tgggatcgat ggcgagcggg tcgacgaggt ggatgacgtc gttgacctcg atttggatca
     2581 gcgccagctg cgggaagtac gtgcgttcgc ggtggaactc ggtgtcgatc gcgtacgcat
     2641 cggccgagca gacctcctcg acgagcgcgt cgagggccga ggcggtggtg atcaggtcgt
     2701 ggggaggggc cggaggtgga gccgccgggc tagccacgct ccatcccggt caccacgtcg
     2761 cgccccgaat cgatccacgc catggtgccg ccggcgatgt tggtgacgtc gcggccgagc
     2821 ggcgcgagga agttgcaggc gcgcatgctg cgcccaccgc tcttgcagat cacgagcacg
     2881 ggttcgcccg acgggacctc gtcgacacgc tgctccagct cgctcagcgg cacgaacagt
     2941 gcgccgggga catggccgga gtcgtactcg tcggcctctc ggacatcgat gaccacgaca
     3001 ccctcggcca gccgttcggc cagctcatcg acgtcgatct cacgtggttc tgtcatggcg
     3061 tcaacctacc tggaagggag cgagatcacc gggttcgtcc gcgtcggcga acgccgcgcc
     3121 cccgtcgact tccacgacgt cgatgccgtc gagcagctcc cggaacgacg acgcaccgtc
     3181 gccgaagcgg tcctcgagcg ccgggaggag ggcgacgggc cacacaccgt tgggcggagc
     3241 cggccggccg tcgacggcga cgaggacggc ctggcgggcc tccacgacgt cgaggagtgg
     3301 gcggacggcg tcggcggtca ccgccggcag gtcgcacgcc aggcagacca cgtgggtcgc
     3361 gccgacgagg aaggcggcgt ggagggcggt caagaccgcc ccgaccgggc cctcgccggg
     3421 ccacaggtcc tcgacgacac ggccgccgag gcccccgtgg gcggacgtcg cgcccaccag
     3481 catgatgtcg gtgagaccgg cggcctcgag ggcgcgtacc gagcgggcac cgagcggggt
     3541 gccggcgacg tcgaggctgg ccttgtcggc gcccatccgg gagctctcgc cgccgacgag
     3601 aacggcgccg atggtggtcg gacggggcgc gcccgtcacc gggtcgaccc gctcattcga
     3661 cgacgccgcc gtacacgtgc tcaccgccgc gggccgggcc gtggctggcg accgactcgg
     3721 cgagatcggc gaggaacgcg tcgacgaccc gatcgtggcc gggcgcgagc atcaggtgca
     3781 ggccgccctg ttggcgatcg aggttccagc cgcgatcgtc catctcgtca cagaccgcgt
     3841 ccatgtcgtg gcggtccgaa ccgaactcga acaccgacat gtcgggatcg ctcgtgatgt
     3901 gcaggccgtc gatggcacgg atcccggctt gcatgcgccg ggtcgcgtcg agcacgcggg
     3961 tggccagccg gaggtagccc tcctcaccga ggtggttgat cgccgcccac gcgccggcga
     4021 tgggcggacc cggccgcgtg cccgcagccg tcgaactcgc gtacagacca ccgggccacg
     4081 agtcgtacat gaagatctgg tgctggtaca gctcccgatc gcggtaggtg atcgtcgaca
     4141 cacccttgaa cacgtagccg tacttgtgga tgtcggcgga cagcgacgtg accccctcga
     4201 cgcggaagtc ccacggtggc accggctcgc cgaggcgctc ccaccagggc agcagccagc
     4261 cgccgagaca ggcgtcgacg tggcagagca gctcgttctc ggcagcgatg gcggcgatct
     4321 cggtcaccgg gtcgatcacg ccgtaggggt agcagggggt ggaggcgacg agaatcgccg
     4381 tgcgttcgtc gatcgcctcc tcgtacagcg ccggaacggc ccgaccatcg aggccgtggt
     4441 cgagccggac catctcgacg ccgaggtagt ggcaggcctt ggcgaacgcg gggtgcgccg
     4501 tgtcggtgca cacgagcgtc gggtgcgtga tgccgcgctg gagcgcccac tccctcgccg
     4561 tgtacacggc gaggaagatc gattcggtgc cgcccgagga caacgacccg gcggacgccc
     4621 cgaacaggga cttggcgtag tcgatgacct cggtctccat cttgaggagc gtctcgtagc
     4681 ggaacgggtt gagtgcgttc tcgtggagga agtcgttggc gacggtgtgc aggagctcct
     4741 cgagctcggg ctcgtcggcg ttgtacacga ggctgaacgc cttgccgtgc cgccagtcga
     4801 tgtcgcgtgg tcgcatctcg gcgagttcag tgaggaggtc cgcgctgctc cggccggtga
     4861 tcgggaaggt cgagtcgccc atggccgcag agcgtaggcg cagcgaccgt gcctagcgca
     4921 gctggcctcc gccatgggag tcccattcgg ggtcgagctg gcggaggaag atcgagagtc
     4981 gctcgatctc gtcctgctcg ccgatctcgg tcgcggcgct caacaggccg ttgatcgctg
     5041 cgagcacgcc gcggttggac tcgtgacgcc accggacgta accgctcccc cgccatccgt
     5101 tgccacgcag cgcgtcgaga cctcggtggt agccgannnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnccgc tcccccgcca tccgttgcca cgcagcgcgt cgagacctcg
     5281 gtggtagccg acgcggtagg ccgcgtaccg ctcgacggga tcgcgcccga gcgccccgag
     5341 cgcggcccag gccgtgctgc tgcgggggta cgcgaccaca acagcggcca cggcgtcgcg
     5401 gtcggagccg gcagcggcca gctgggcact gagcgccgca ggctccggcg gaaggacggt
     5461 ctcgggagga cccgagggcg agaggttgat gtcggacatg gcgcgaccct acgtcagcgg
     5521 cgtcacgtcc acgtcggcgt cgtcgggcga acctccgtcg agttcgctcg ggacgtcggc
     5581 atcgtcgggg tcgagctcgt cgcgctcgag tgcggcgtca cgctcggtcg gctgaccgtg
     5641 gccccaccgg ccggtgacct tgccccgcat ctcgtcgatg acccggcggg cgtcggcgcg
     5701 cagccgctcg gcacggtcgc ccatcgcttc gtcctcatcg tcggcctcga gctggtcgag
     5761 gtatccgaca gcgttcaagt agccctgctc catcagctcg tcggtgtggt cgaagtcgtc
     5821 gaagcgcagc tcgggccgct cgccgggcgg caggacgatc gcttcgacat tgcgcggcag
     5881 cgacgccagg tcgcgggaga accggccgtt gcgggcgatc cagtaggcga tcagcgcggc
     5941 gtcggccggc cgggtgatcg ccggcttcgg tttgccgtgc agaccgacgt ggagcacgta
     6001 gatcttgcgg gcaccgagtt cgatcgcacg agcgatcggc acgttgttga gcaccccacc
     6061 gtcgaggaac cggcgctcgc cgatcgtcac aaccgggtag accgcgggca gcgcggccga
     6121 cgccaggatc ggttcgagca acggaccctc gctgaaccac gtttcggccc cggcgtcgac
     6181 gtcggtcgcc acgcactgga acggcaccgt cagctcgtcg aacctctccc ggcccgagag
     6241 gaatcgccgg atcgtccgac tcagcccgtc gttcgggttg agcgcggagc ccttgcggag
     6301 gagctgcacg gggttgggga tccacgagcc cggcatgagc gtttcgggct cgtgctggat
     6361 ctcggtccag atctgctcga ggtgacggac acccgccatc gaggggtcga cggcgaacgc
     6421 cccaccgttc aaggccccga ccgagcagcc gagcacgatg tcgggaacga tcccgcgctc
     6481 ggcgagcgcc cggagctggc ccacctggat ggcacccaga ttgccgccac cgccgaggac
     6541 cacggcggtc ctggggcggc ggcggacgag gggacggacg acccgggcca tggggcgtga
     6601 acctaccgcg gggactccgg acccgagggc cacgactcga tcagctcggc gaacgcggtc
     6661 ggcccgctca ggtgcacgcc gtggtcggcg ccctcgatcg cgacgagccg gcatgactcg
     6721 atgccggccg ccgcctcggt ggcggaccgc cggtggtgct cactcgactc ggtgccaagg
     6781 ccgatcacca ccggcagggt gatcagcgac aggtcgaccg cggcctcggg actgcgggcg
     6841 ttgaacatgt cggcctgcag ggcgcgaccc tcggcccggc gttcggcctg catcgtcgcc
     6901 gggaggcgct cccacagccg gtcaccaagc acgccccgca tgaaggcttc ggctgcaacg
     6961 tcgggggcga ggtcgagcag cccgctcccc cgcgacgtgt accagtcgtt ccacgccagg
     7021 ggtgcctccc agacgacggc gccgaccgcg agttcctcga tgccggggcg ggcaagggcg
     7081 tgcaacgcga tcaggccacc ggcgctgtgg cccaccacga ggcgacgctc gccgttcgcc
     7141 gcgatcaact cggtcagcac ggcgacaaga tcgtcgacct gcgtcgcgaa ctcgcgtacc
     7201 ggcggcacgt cacgcgaccc ggcgtagccg cgccggtcgt aggccacgat gtcgcgaaac
     7261 ggcaggtgcc gggcggtccg acggaacccc gccgcgcggt ccatggcacc gtgcacgatc
     7321 accagcggcg gtgccgaagc ggaagcagct cctgagggac gaaggagcga gcctgagcgg
     7381 aggcggccgt cgccatctgg gagcggtggg ccgtcgggct cgccggccgg tcgatagtgg
     7441 tcggcggcca ggccggcgat cgtgatcgcc tcgggcgcgg gccgacggac gacgtcgcgg
     7501 acggactcgc tcaaggaacg cgaacgagtc ggagctggtc ggtggcccgg gtgcggttct
     7561 gccgggcacc ggcgagcacc gccacgtagt cgcccgaccg gatgtgaccg gcgtcgcggg
     7621 ccgccaccac cagaccgttc atcaggtcgc cctcggagat gtccggagcg atgagcggcg
     7681 tcgttcccca gctcagggtc agctgccgtt cggttcgggg gttggggctg aacccgagga
     7741 tcggcatcga cggccggaac cgggcgatcg atcgcaccgt gaagcccgac tcgctgatgc
     7801 acaggatggc cgaggcgccc atctcggtcg ccgcccgcca gcccgcgccc gtcatcgcat
     7861 cggtgaggcg cgtctcgtcg tcggacacgt ggatgctgcg gagcgcacgc aggcgacgag
     7921 cccacccttc gtagtcgaac tcggcgtcgg cccgttcgca gatgcgggcc atcgtcgcca
     7981 ccgagttgac cgggctcgcg ccgatcgccg attcggcgct caacatgacc gccgacgaac
     8041 cgtcgaagat cgcgttcgcc acgtcggaca cctcggcccg ggtcggcgtc gccgcggtga
     8101 tcatcgactc gagcatctgg gtcgcggtga tgaccggacg gccgtaggcg atgcactccc
     8161 ggatgatgct cttctgcagg tggggcagct cctcgatgcc catctcgatg ccgaggtcac
     8221 ctcgggcgac catcacggcc cccgacgcct cgatgatgcc ctccaggttc tccacggcgg
     8281 cgcgggtctc gatctttgcg atgacgagcg gccctcgcgg atagggctcg gtgccgacac
     8341 ggcggatgtc gtgggccgac cgcacgaacg acacggcgat catgtcgacg ccctcctcga
     8401 cgaacgcgtc gagcttgtac agatcctcgg cggtgggcgt cgcgaggccg aggcggtccg
     8461 acgggatgtg caaccccggc cgaccctgaa cgagaccacc gtaggtgacc tcggccttgg
     8521 ccgaggtgcg atcgaccgac gacacggtca ggcggacggt gccatcgccc acggcgaggc
     8581 tgtcgccggg ttggagatca acgaggagat cggtgtagtc gacgccgagg ctcgacgccg
     8641 acgaggtcgg gtcgccggga cggagcacca ccgtcgagcc gggcacgagg gccacaccgc
     8701 cctcgtcgaa ctgcccgagc cgcaccttcg ggcccgggag gtcggccagg atcccgacga
     8761 cgcggttggc gtccgccgcg atctggcgga tggtgcggta acgctcgacc gcatcggaca
     8821 gcgacccgtg cgccagcccg attcggaaca cgtccacccc cgcctcgatc atctggcgga
     8881 tgacgaccgg atcgtccgac gccgggccga cggtggcgat gatcttcgtg cgacgtggca
     8941 tgtgacttcc cttcgattcg aggccaaccg gctcgattcg gtcgcttcgc gtccgacctg
     9001 cggttcggaa cctcggcctc caacctaccg gccgggactt tcgccgcctc ggcatatcga
     9061 accggcaacg atcagttcac ccaagcggca cccgggccct acggcccaac gggcctgagc
     9121 aatcggcaga tgaactgtag aggggcacat cagctcccga tcagtgtcat gtcaggtcaa
     9181 cgacgtacgg aaatccgtcc cgccgtcggc ccggcagtgc gagagcaagg aagcggtcgc
     9241 caccccaccc gttcaatccg ctcaaaggag cacacaatgg aaacgtcaac ccccacctct
     9301 cgttcggcgc gccgtcgtcg tcgaatcgcc gtcgccgtca tcgccggcgt cggcacgttc
     9361 ggcgtgatcg gagcatcggc cgccagcctc ggtggcatca ccggttcgag cctcggagcg
     9421 gacgctgcgg tcatcggctc gtgcgacacc gacggtgtca gcctcaccta caccaacacc
     9481 tacgacgcga ccctcggccg ctaccagacg accggcgtca acgtcaccgg catcgccgcc
     9541 gcctgcgccg gcaaggcact ggccctcacc ctgaaggaca ccggcggcgt ctccctcggc
     9601 tcgggcacgg tcgcttcgat cgtcggcacg tcgtcgagcg tcaccctcac cgcgcccggc
     9661 gccaacgcga acgccgtcgt cggcgccgcc gtcgtcatca gcggctgatg tcccggcggg
     9721 ctcgacgccc gtccctcggc atcgtcggac tggtcgtcgg cgcacagctg atggtgagcg
     9781 ggttcggggt ggcgtcagcc gcctccctcg gcgggctcac cacggcgcgt ctcaccgcca
     9841 acaccgtcgc gggcacctcc ggtgccccga cggtggtggc gtgggagaac ttcaacggca
     9901 ccaccggaac caacctcggt ggcaccacca ccgacggcgg cgcg
//
LOCUS       DAOSOA010000100         9906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_6810_length_9906_cov_6.453253, whole genome shotgun sequence.
ACCESSION   DAOSOA010000100 DAOSOA010000000
VERSION     DAOSOA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6810_length_9906_cov_6.453253"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..3455
                     /locus_tag="PKY13_08150"
     CDS             312..3455
                     /locus_tag="PKY13_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT79944.1"
                     /translation="MHDDRSIIEGRLRRLAFERLNPAIHGARVPLTVTAWEVPGEPVT
                     AAVAAAQQYEPFAIGSMFGRPWGTTWFRMSGTVPEDWAGRAVDAVIDLGFGHSPGFQS
                     EGLLWEVADDGSWAPVRGLHPLNRDVFVSSAATGGEAVQYVVEAASNPAIAHTRPDPN
                     SDLLTAATGPIYGLSRAELVVVNTDVVALIEDIRALNGLMLELPLDQPRRHEILRAIE
                     AMMDRLLPDDIPGTAAAARAELAGVLAQPASASAHRISAIGHAHIDSAWLWPIRETIR
                     KCARTFSNVLALMDRYPDFRFGCSQAVQYEWMREHYPSVFEGIKRRVADGRWVPIGGM
                     WVEADTNLAGGEALIRQITHGQRYFEEQFGVTCTEVWIPDVFGYPASLPQIMQVCGLD
                     RFLTQKLSWNKTNRFPHHSFWWEGIDGSTVFTHFPPVETYNANFQGNELAHAVRTFTD
                     KGRSTRSLMPFGHGDGGGGPTAQMMEQYLRVRDLEGSPRVEIESPEAFFDAAIAEYPD
                     APRWVGELYFEMHRGTFTSQAKTKAGNRRCELLLREAEIWCAAAYGGGSAYPAADLDR
                     LWKTVLLHQFHDILPGSSIGWVHREAEVAYGQVTDELISIIDRAAAALAGDRTSLANP
                     APHPVDGVVVVDAPSLVGDPACQILGDGRVAAVVSLPALAVTIADPLDLAHPPVQVSA
                     TTLDNGLLRVTLDEQGLIVSIIDVEEGREIVAPGTRANLLQLHQDLPNEYDAWDIEEF
                     YRKQVVDLDAVDTVEVLDRGPLIGRVQVTRTFRSSSVTQVLELRAGSRRLDVVCDVDW
                     HEHEQMLKVAFPVDVHADEVTREIQFGHLRSPIHTNTSWDAARFEVCAHRWIDVAEPG
                     YGIALLNNCKYGHDITRSRSEGPDGEVPTTTLRLTLLRGARYPDPEQDQGRHHFTYSL
                     LPHTGSLSQAGVVAEGYRLNCPVRVVEPLRADERSGRLVDDGASVVTSDHPGVVVETV
                     KAADDGGGDLIVRCYEAWGGRARAQLGFGFDVADVRVVDARERPGGAASALVGNGPAV
                     EIVVRPFQILTLRVTPR"
     assembly_gap    3554..3653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3654..4286
                     /locus_tag="PKY13_08155"
     CDS             <3654..4286
                     /locus_tag="PKY13_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79945.1"
                     /translation="LRTATDPQTSPAVVVVAIVVGVLLVGAVGWFTLGGDSRGDVRAN
                     GPATTLTPVARPAIPASGQTRATGDGWVTYRTPDGSWLVDLAVVPSAPGPLPSNPTIQ
                     ISYGKTDFGRVGFGWSAGQVAPGTDETALLVEIMKGIALSRGATGIEPTVLPTDRGNV
                     VTARLQLDSGSALFVLRSVDRRPLVAMVIADLGEPDPAIANRMLDSFRPV"
     gene            4323..5495
                     /locus_tag="PKY13_08160"
     CDS             4323..5495
                     /locus_tag="PKY13_08160"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT79946.1"
                     /translation="MAAPAARPAPGWIHPTDLGGAVALAAFCIVVGRKQPLTFPGARP
                     ADLAQAVIIAVACLGLAFRRPMPSVSVLIGSAGVAVYLGCSYTGGPVLLVPLVGMFFA
                     GYHLTRARVWQLAGAFAVFLAVAVVATDAPAAQAWVWLLAWPPMAAALGLAGAARRRH
                     ELDLAERDEEEDRRQHAEARRQLAEARLQTARELHDVIGHSLASITILAGAGSRRVET
                     DPAAAKEVLDEIRAVSATALSDVRQALDALNDPAQAFLGDDGADLAHLIERLRRVGLV
                     VDDRIEVDPASLDPAVATALHRIAQESLTNVMRHSGSLHASIAVQQSHGGVLMTVSDD
                     GNGAVDLQEGRGIQGMRRRAEELGGRLSIVGSGTGETGRSGLSVVVWVPVRVAVEP"
     gene            5492..6199
                     /locus_tag="PKY13_08165"
     CDS             5492..6199
                     /locus_tag="PKY13_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT79947.1"
                     /translation="MTRTEPITVGVADDQALIRRGIRLLVENEPDLVVVGEAANGLEA
                     IELVRRHRPDVMLIDVHMPEMDGLEATEVIAADPELAATGVIVLTTFELDEYVDRALR
                     GGASGFLLKDTEPDLIIQAIRLVANGEALIDPAVTKAMIARFSEASASSTEAAPGPID
                     ESLAVLTTREREVLQLVAAGLSNAEIAESLFISVATARTHVGRMLMKLGARDRAQLVV
                     IAFQTGLVAPGGHPRTH"
     gene            6215..6781
                     /locus_tag="PKY13_08170"
     CDS             6215..6781
                     /locus_tag="PKY13_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOT79948.1"
                     /translation="MRPLRYSINVTLDGCCLHEAGLPPDEESMRYWAAEMERADALLF
                     GRVTYEMMESAWRKPTTGAWPDWMDDTQVPFADGIDRAKKYVVSSTLSGVDWNAELVR
                     GDLGPAVQRLKEESGNGLWVGGVTLPTALAELELIDEYEFIVHPVLAGHGPTLLAGLS
                     ERVQLELVGRHEFGSGAVALRYRPTRAT"
     gene            6930..7691
                     /locus_tag="PKY13_08175"
     CDS             6930..7691
                     /locus_tag="PKY13_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT79949.1"
                     /translation="MTSTTFPPAAPAGTAVARAVNAVKHYGEGDTVVKALDGVTVEFD
                     RSRFTAIMGPSGSGKSTLMHCVAGLDTLTSGQALIGDVDLGTLADKDLTILRREQIGF
                     IFQAFNLIPTITALENIVLPLRLGGKTADQDWLDSVVDTVGLRDRLSHRPSELSGGQQ
                     QRVAVARALVSRPQIVFADEPTGNLDSRSGAEVLSFMRRSVDEFGQSIAMVTHDPVAA
                     SYAHRVLFLADGKVVGHLDDPTAASVLDVMRGLGD"
     gene            7694..>9906
                     /locus_tag="PKY13_08180"
     CDS             7694..>9906
                     /locus_tag="PKY13_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT79950.1"
                     /translation="MIKLSLQNTWAHKRRLVGTFLAVLIGVSFLSGTLVLGDTLRDNF
                     DKLFKAGYAGIDVIVRPERPADGSDDDTGFSAKTIDASLADTIRGIDGVRAVAAQTTG
                     FAQVIGADGERIGGSGPPTLAANWDPDDGLNPYNLVKGRAPEGPEEVILDDETVETGK
                     LKLNGTATLLMPTPLEVTIVGIAEFGDGSSKVGVTYAFFSESGTAAHLTREPGQVNQI
                     AVAAKRGVTEEALLKRIEPVIGSGNEALTGTAAADEAVSDINADFLGFITTFLVVFAG
                     IALFVATFSIYNTFSIIVAQRSRESALMRAIGATRGQVLQATMVEALVIGVLASVVGL
                     FGGIGVAALLKAAFDAFGLALPAGGLTIKTSGIVIAIVVGVVVTLGAAIVPARRGSRV
                     RPIEALRASALESEVPRTRRTVIGVVLAIVGVGVLLWSVLAEPDNMINYAGPAAFVIL
                     VAMLVLGPVVARPVAGLLGAPIAKAGGITGELSRDNAIRNPRRTASTAAALLVGVAIV
                     VLFSVFIASLKSLITDSVDRTFGGDLVVANGGFGIPTLPPELAGQIAGIDGVKSALGY
                     GVAQVGVDGEDPDFYGAVDVPELAKVLDLDIVQGSAADFGDGDVGISEETAKDNRLSL
                     GDSLELSYPDGAKEQGTVAMVYRNADIVGSYLFSRATTAPHQTIKADAVVFVTLDEGA
                     SPAKARKDIKQFTDPIGKPKIQDKQEYTDSVAANLDALLGIIIVMLALAIIIAAMG"
BASE COUNT         1455 a         3443 c         3354 g         1554 t
ORIGIN      
        1 ccaggccgtg tcgttggcga gcccgccctt ccggaagtgg cgtttgatgc ccgtgtcctc
       61 cgccggcggt gctccgggct cgaggagctc gtactcctgg aagccggtca cgggcgcgtc
      121 accgggtgat cgggaagaat gcgcatcgtg gtcggcgtga cgtctcgggg gaagggcatg
      181 agatctcttg gtgcggggcc gcgggcgcgg cgtgcgaagg ttcagggtaa cgagacgagc
      241 ccgagagtgg aacccggagt tgtcgaaaat gactgagatg ttgcgagaga gtgggaacgg
      301 gcaggtcacg catgcatgac gatcgatcga tcatcgaagg gcgcctccgc cgactggcgt
      361 tcgagcgcct caatcctgcc atccacggcg cccgggtccc gctcaccgtc accgcctggg
      421 aggtccccgg cgagccggtg accgcggcag tggccgcagc acagcagtac gaaccgttcg
      481 cgatcggttc gatgttcggt cggccgtggg gcaccacctg gttccggatg tcgggcaccg
      541 tgcccgagga ctgggccggt cgcgccgtcg acgccgtcat cgacctgggg ttcgggcaca
      601 gcccggggtt ccagagcgag ggcctcctct gggaggtcgc cgacgatggg tcgtgggcgc
      661 cggtgcgagg cctccacccc ctcaaccgcg acgtgttcgt gagttcggcg gcgaccggtg
      721 gggaagcagt gcagtacgtg gtcgaggcgg cctccaaccc cgcgatcgcg cacacgcgtc
      781 ccgatcccaa cagcgacctg ctgacggccg cgaccggtcc gatctacggc ctgtcccggg
      841 ccgagctcgt ggtggtcaac accgacgtcg ttgcgctcat cgaggacatc cgcgccctca
      901 acgggctcat gttggagctc ccgctcgatc agccgcgacg ccacgagatc cttcgcgcca
      961 tcgaagcgat gatggaccga cttctccccg acgacatccc cggcaccgcg gcagctgctc
     1021 gagccgagtt ggcgggggtc cttgcccagc cggcgtcggc gtcggcacat cggatctccg
     1081 cgatcgggca cgcccacatc gactccgcct ggctctggcc gatccgcgag accatccgca
     1141 agtgcgcccg cacgttctcg aacgtgctgg ccctcatgga tcgctacccc gacttccggt
     1201 tcgggtgctc ccaggccgtc cagtacgagt ggatgcgtga gcactacccc tcggtgttcg
     1261 agggcatcaa gcggcgcgtc gccgacgggc ggtgggtccc catcggcggc atgtgggtcg
     1321 aggccgacac caacctcgcc ggcggtgaag ccctgatccg gcagatcacc cacggccagc
     1381 gctacttcga ggagcagttc ggcgtcacgt gcaccgaggt ctggatcccc gacgtgttcg
     1441 gctaccccgc gagccttcct cagatcatgc aggtctgcgg gctcgatcgg ttcctcaccc
     1501 agaagctgtc ctggaacaag acgaaccggt tccctcacca cagcttctgg tgggagggca
     1561 tcgacggatc cacggtcttc acccacttcc cgcccgtcga gacgtacaac gccaacttcc
     1621 agggcaacga gctcgcgcac gcggtgcgga cattcaccga caagggccgc tccacgcggt
     1681 cgctcatgcc gttcggccac ggcgacggcg gtggtggtcc gacggcccag atgatggagc
     1741 agtacctgcg ggtccgcgat ctcgaaggct cgccccgcgt cgagatcgag tcacccgagg
     1801 cgttcttcga cgcggcgatc gccgagtacc ccgacgcgcc ccgttgggtc ggtgagctct
     1861 acttcgagat gcaccgcggc acgttcacgt cgcaggcgaa gaccaaggcc ggcaaccggc
     1921 ggtgtgagct gctcctccgc gaggccgaga tctggtgcgc cgcggcgtac ggcggcgggt
     1981 ccgcctaccc cgcggccgac ctcgaccggc tgtggaagac ggtgctcctg caccagttcc
     2041 acgacatcct tcccggttcc tcgatcggct gggtgcaccg cgaggcagag gtcgcctacg
     2101 ggcaggtgac cgacgagttg atctcgatca tcgaccgtgc tgccgcggcg ctggccggcg
     2161 atcgcacgtc gctggccaat ccggcccccc accccgtcga cggggtggtc gtcgtcgacg
     2221 ccccctcgct ggtcggcgac ccggcctgcc agatcctcgg cgacggacgc gtcgcggcgg
     2281 tggtgtcgtt gccggcgctc gccgtcacga tcgccgaccc gctcgacctt gcccacccgc
     2341 cggtgcaggt gtcggcgacg accctcgaca acggtctcct ccgcgtcacc ctcgacgagc
     2401 aggggctcat cgtgtcgatc atcgacgtgg aggagggtcg cgagatcgtc gctccgggga
     2461 cccgcgccaa cctgctccag ctccaccagg acctgcccaa cgagtacgac gcgtgggaca
     2521 tcgaggagtt ctaccggaag caggtcgtcg acctcgacgc cgtcgacacc gtcgaggtgc
     2581 tcgaccgtgg tccgctgatc ggccgggtcc aggtcacgcg gacgttccgt tcgtcgagcg
     2641 tgacgcaggt cctcgagctc cgcgccggat cccgccgact cgacgtggtc tgcgatgtcg
     2701 actggcacga gcacgagcag atgctcaagg tcgcgttccc cgtcgacgtc cacgccgacg
     2761 aggtgacgcg cgagatccag ttcggccatc tccgcagccc gatccacacc aacacgagct
     2821 gggacgcggc gcggttcgag gtgtgcgccc accgctggat cgacgtcgcc gagccgggct
     2881 acggcatcgc cctcctcaac aactgcaagt acggccacga catcacgcgc tctcgctcgg
     2941 aaggtcccga cggcgaggtg ccgaccacca cgctgcggct gacactgctc cgcggggccc
     3001 gctaccccga ccccgaacag gaccagggcc gacaccactt cacctactca ctgctcccgc
     3061 acaccggaag cctgtcgcag gcgggcgtcg tcgccgaggg gtaccggctc aactgcccgg
     3121 tgcgcgtggt cgaaccgctc cgggccgacg aacggtccgg gcgactggtc gacgacggtg
     3181 cgagcgtcgt gacgagtgac catcccggtg tggtcgtgga gaccgtgaag gccgccgacg
     3241 acggcggtgg cgacctcatc gtccggtgct acgaggcctg gggtggacga gcgcgcgccc
     3301 aactcgggtt cggcttcgac gtcgcggacg tccgggtggt cgatgcccgg gagcggcctg
     3361 ggggcgcggc ctccgcgctg gtgggaaacg gaccggcggt ggagatcgtg gtgcgcccgt
     3421 tccagatcct cacgctgcgg gtgacaccgc gctgaatgtt ctcaattcct ctcgtcggcg
     3481 tgtcgattcc cagtggagtc gtcccccacg atcaacgggg ccgcgcacga ggagttccgc
     3541 cgttgctcac ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctccgga
     3661 ccgcgaccga tccgcagacg tcgccggcgg ttgtggtggt ggcgatcgtc gtcggggtac
     3721 tgctcgttgg ggcggtgggg tggttcaccc tcggcggcga cagccgtggg gacgtccgcg
     3781 ccaacggtcc agcgacgacg ttgacgccgg tcgcccgccc ggccatcccc gcttcgggtc
     3841 agacccgtgc gaccggcgac gggtgggtca cctaccgcac gcccgacggc agctggctcg
     3901 tcgatctcgc ggtcgttccg tcggcgccgg gcccgctgcc gtcgaatccg acgatccaga
     3961 tctcttacgg caagaccgat ttcgggcgcg tcgggttcgg gtggagcgcc ggccaggtcg
     4021 ctccgggcac cgacgagacc gcgctgctcg tcgagatcat gaagggcatc gcgctcagcc
     4081 gtggtgccac gggcatcgag ccgaccgtcc tgccgacgga ccgcggcaac gtcgtgacgg
     4141 cgaggctcca gctcgacagc ggttcagccc tgttcgtgtt gcgttcggtc gatcgccggc
     4201 cgctcgtcgc catggtcatc gccgacctcg gagagcccga tcccgcgatc gccaaccgga
     4261 tgctcgattc gttccgtccc gtctgacggt cgcggggtcc ccgctgtgca agggtgatgg
     4321 ccatggccgc accggcagcc cgccccgctc ccggctggat ccaccccacc gacctcgggg
     4381 gtgcggtcgc gctcgcagcg ttctgcatcg tcgtcggccg caagcagccc ctcacctttc
     4441 ccggcgcccg cccggcggat ctggcgcagg ccgtgatcat cgccgtggcg tgcctcgggc
     4501 tggccttccg tcgtccgatg ccgtcggtgt cggtgttgat cggcagtgcc ggcgtcgcgg
     4561 tgtacctcgg ctgcagttac accggtggcc cggtcctcct ggtgccgctc gtcggcatgt
     4621 tcttcgccgg ataccacctg acccgcgctc gggtctggca actcgccgga gccttcgccg
     4681 tgttcctggc ggtcgcggtg gtggccaccg acgccccggc ggcgcaggcg tgggtgtggc
     4741 tgctcgcctg gccgcccatg gctgcggcgc tgggcctggc cggagcagcg cggcgacgcc
     4801 acgagctcga cctcgccgaa cgcgacgagg aagaggatcg ccgtcaacac gccgaggccc
     4861 gccggcagct cgccgaggcg cggctccaga cggcgcggga gctccacgac gtcatcggac
     4921 actcgctcgc cagcatcacg atcctcgccg gcgccggttc acggcgggtc gagaccgacc
     4981 cggccgcggc gaaggaggtg ctggacgaga ttcgcgccgt cagcgcgacc gcattgtccg
     5041 acgtgcgaca ggcactcgac gcgctcaacg acccggccca ggcgttcctc ggcgacgacg
     5101 gcgccgacct cgcccacctc atcgaacggc tccgtcgcgt cggcctcgtc gtggacgacc
     5161 ggatcgaggt cgacccggcc tcgctcgacc cggccgtggc gacggcgctc caccgcatcg
     5221 cgcaggagtc gttgaccaac gtgatgcggc attcgggctc gctccacgcg tcgatcgccg
     5281 tccagcagtc gcacgggggc gtgctcatga ccgtgtccga cgacgggaac ggcgcggtcg
     5341 atctgcagga gggccgcggc atccagggca tgcggcggcg ggccgaggag ctggggggtc
     5401 ggctctcgat cgtcggcagc ggcaccggcg agacggggcg gtcggggctc agcgtggtgg
     5461 tgtgggtgcc cgtccgtgtg gcggtggagc cgtgacgcgc accgaaccga tcaccgtcgg
     5521 cgtggccgac gaccaggcgt tgatccgtcg cggcatccgg ctgttggtcg agaacgagcc
     5581 ggacctcgtg gtggtcggcg aggcggcgaa cggcctcgag gccatcgagc tggtgcgacg
     5641 ccaccgcccc gacgtgatgc tgatcgacgt ccacatgccc gagatggacg ggctggaggc
     5701 gaccgaggtg atcgccgccg accccgagct cgccgccacc ggggtgatcg ttctcacgac
     5761 gttcgagctc gacgagtacg tggaccgcgc gctgcgcggc ggcgcgtcgg gcttcctcct
     5821 gaaggacacc gagcccgacc tgatcatcca ggccatccgg ctggtggcca acggtgaggc
     5881 actgatcgat cccgccgtca cgaaggcgat gatcgcccga ttctccgagg cgagcgcgtc
     5941 atcgaccgag gccgcgccgg gaccgatcga cgagtcactc gcggtgctca cgactcgtga
     6001 gcgcgaggtc ctgcagctgg tggcagcggg cctgtccaac gctgagatcg ccgaatcgct
     6061 gttcatctcg gtggcgacgg cgcgcaccca cgtcgggcgg atgctcatga aactcggcgc
     6121 ccgcgatcgc gcccagctcg ttgtcatcgc gttccagacc ggcctcgtcg ctcccggcgg
     6181 ccacccgcga acccactgac gaaagggcgc acccatgaga ccacttcgat actcgatcaa
     6241 cgtcaccctc gacggttgtt gccttcacga ggcaggcctc ccccctgacg aggagtcgat
     6301 gcgctactgg gccgccgaga tggaacgagc cgatgcgctg ctcttcggcc gagtgaccta
     6361 cgagatgatg gagtcggcgt ggcggaagcc gacgacgggt gcctggcccg actggatgga
     6421 tgacacgcag gttccgttcg ccgacggcat cgaccgggcg aagaagtacg tcgtgtcgag
     6481 cacgctgagc ggggtcgact ggaatgccga gctggtgcga ggcgacctcg gaccggcggt
     6541 tcaacggctc aaggaggaat ctggcaacgg cctgtgggtg ggtggcgtga cgctccccac
     6601 ggcgttggcg gaactcgagc tgatcgacga gtacgagttc atcgtgcacc cggtgctcgc
     6661 cggacacggg ccgacgctgc tcgccggtct gagcgagcgc gtgcagctcg agctcgtcgg
     6721 tcgccacgag ttcgggtcgg gggcggtcgc cctgcgatac cgccccacac gagcgacgta
     6781 gcgcccgcgg ctgcggcgcg ctgtcgggct caccacagca ccgggcgggg ggacaacccc
     6841 cgtcgcgtac gtcagctgac gtacgcgacg cccgacgatc atccttgtgg ccgatgtcgc
     6901 tgtcggggga gacgtcgata cttgtcttca tgaccagcac caccttcccc ccagccgccc
     6961 ccgcgggcac cgccgttgcc cgggccgtca atgccgtcaa gcactacgga gagggcgaca
     7021 ccgtcgtgaa ggcgctcgac ggtgtgaccg tcgagttcga ccgttcgcgc ttcacggcga
     7081 tcatgggccc gtcgggctcg ggcaagtcga cgctcatgca ctgcgtcgcc ggcctcgaca
     7141 cgctcacgtc ggggcaggcc ctgatcggcg acgtcgacct cggcaccctc gccgacaagg
     7201 acctcacgat cctccgccgt gagcagatcg ggttcatctt ccaggcgttc aacctgatcc
     7261 ccacgatcac agcgctcgag aacatcgtcc tgccgctccg gttgggcgga aagaccgccg
     7321 accaggactg gctcgactcc gtcgtcgaca ccgtcgggct gcgcgaccgc ctctcgcacc
     7381 gaccttccga gctctcgggt ggtcagcagc agcgtgtggc cgtcgcccgt gcgctggtca
     7441 gtcgtccgca gatcgtcttc gccgacgaac ccacgggcaa cctcgactca cgctccggcg
     7501 ctgaggtgct gtcgttcatg cgccgcagcg tcgacgagtt cggccagagc atcgccatgg
     7561 tcacccacga ccccgtcgca gcgtcgtacg cccaccgggt gctgttcctc gccgacggca
     7621 aggtcgtcgg tcatctcgac gacccgaccg cggcctcggt gctcgacgtc atgcgcggac
     7681 tgggcgactg atcgtgatca agctctcact ccagaacacc tgggcgcaca aacgacggct
     7741 cgtcggcacc ttcctcgcgg tgctgatcgg cgtcagcttc ctgtcgggca cgctcgtcct
     7801 cggcgacacg ctccgggaca acttcgacaa gctgttcaag gccgggtacg ccggcatcga
     7861 tgtcatcgtt cgccctgagc gtccggccga tggcagtgac gacgacaccg gcttcagcgc
     7921 caagacgatc gacgcatcgt tggccgacac gatccgcggc atcgacggag ttcgtgcggt
     7981 cgccgcccag accacgggct tcgcccaggt catcggggcc gacggcgagc gcatcggtgg
     8041 cagcggcccg ccgacgctgg ccgccaactg ggaccccgac gacggcctca acccgtacaa
     8101 cctcgtcaag gggcgcgccc ccgagggtcc cgaagaggtc atcctcgacg acgagacggt
     8161 cgagaccggc aagctgaagc tgaacggcac cgcgacgctg ctcatgccga cgcccctcga
     8221 ggtgaccatc gtcggcatcg ccgagttcgg tgacgggtcg agcaaggtcg gcgtcacgta
     8281 cgcgttcttc tccgaatccg gcaccgcagc gcacctcacc cgagagccgg gacaggtcaa
     8341 ccagatcgcg gtggccgcga agcggggtgt caccgaagaa gcgttgctga agcggatcga
     8401 gccggtcatc ggttcgggca acgaggcgct cacgggcacg gccgcggccg acgaggccgt
     8461 ctccgacatc aacgccgact tcctcggatt catcaccacc ttcctcgtgg tcttcgccgg
     8521 gatcgcgttg ttcgtggcga cgttcagcat ctacaacacc ttctcgatca tcgtcgccca
     8581 acggagccgg gagtcggccc tcatgcgggc gatcggcgcg actcgtgggc aggtcttgca
     8641 ggccacgatg gtcgaggccc tggtgatcgg tgtcctcgcg tcggtcgtcg gcctgttcgg
     8701 tggcatcggc gttgctgcgc tgttgaaggc agcgttcgac gcgttcggtc tggcgttgcc
     8761 ggccggcgga ctcaccatca agacgtccgg catcgtgatc gccatcgtcg tcggggtggt
     8821 cgtcaccctg ggcgccgcga tcgtcccggc tcgtcggggc agccgtgtgc ggccgatcga
     8881 ggcactgcgg gcgtcggcgc tcgaatccga ggtgccccgc acccggcgca cggtcatcgg
     8941 cgtggtgctc gccatcgtgg gcgtcggcgt gctgctgtgg tcggtgctgg ccgagcccga
     9001 caacatgatc aactacgccg gcccggctgc attcgtgatc ctcgtcgcga tgctggtgct
     9061 cggtccggtg gtcgcccggc ccgtggccgg cctgttgggg gcgccgatcg ccaaggccgg
     9121 cggcatcacc ggcgagctgt cgcgggacaa cgccatccgc aatccgcgac gcacggcctc
     9181 gacggcggcc gcgctgctcg tcggcgtcgc catcgtggtg ctcttcagcg tgttcatcgc
     9241 ttcactgaag agcctcatca ccgactcggt cgaccgaacc ttcggcgggg atctggttgt
     9301 cgccaacggc gggttcggca tcccgaccct tccgccggaa ctggccggcc agatcgccgg
     9361 catcgacggg gtgaaatcgg ccctcgggta cggcgtcgcc caggtcggcg tcgacggcga
     9421 ggacccggac ttctacggcg cggtcgacgt gcccgagctc gccaaggtcc tcgacctcga
     9481 catcgtgcag gggtcggccg ctgacttcgg ggacggggat gtcggcatca gcgaggagac
     9541 cgcgaaggac aatcggttgt cgctcggcga ttccctcgag ctgtcctacc ccgacggcgc
     9601 gaaggagcag ggcaccgtgg ccatggtgta ccgcaacgcg gacatcgtcg gcagctacct
     9661 gttctcgagg gcgaccaccg caccccacca gaccatcaag gctgatgccg tggtcttcgt
     9721 gacgctcgac gagggggcgt cgccggcgaa ggcacgcaag gacatcaagc agttcaccga
     9781 tccgatcggc aagcccaaga tccaggacaa gcaggagtac accgactcgg ttgcggccaa
     9841 cctcgatgcg ctcctcggca tcatcatcgt gatgctcgcc ctggcgatca tcatcgccgc
     9901 catggg
//
LOCUS       DAOSOA010000101         9665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7029_length_9665_cov_6.190323, whole genome shotgun sequence.
ACCESSION   DAOSOA010000101 DAOSOA010000000
VERSION     DAOSOA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7029_length_9665_cov_6.190323"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKY13_08185"
     CDS             <1..456
                     /locus_tag="PKY13_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOT79951.1"
                     /translation="YDFRLLKGVVDVNNEQFERVVRKVVAAVGGDVSGRHISAWGLTF
                     KARTDDTRMSPAIEVIKRLQALGAEVTAYDPAIRMDIEGITVANDPMTACKGSELLMI
                     LTEWDEFKWVDLDEVAEQMTERRIVDTRNLLDRSRAQRAGFEYQGIGRR"
     gene            585..1529
                     /locus_tag="PKY13_08190"
     CDS             585..1529
                     /locus_tag="PKY13_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT79952.1"
                     /translation="MRVVLTGGAGFLGFHLSEILLGRGDEVVCVDNLISGTQSNVDAL
                     SRHDGFEFLLQDASVPYEVAGPVDAVMHFASPASVPDYLANPLETLRVGSNGTFNALD
                     LARAHDARFLVASTSEIYGEPLVHPQPESYWGNVNPNGPRSVYDEAKRFAEAVTAAYR
                     RTYGTDTSIVRFFNTYGPRMRPEDGRVVTNFIVQALLGKPLTIYGDGSQTRSFGYVGD
                     IAAGTIALLDSGHAGPMNLGNPDEFTMIELADLVLELTGSTSDLAFEPLPEDDPTQRR
                     PDISLARDVLNWEPTTALREGLVPTIAYLRELLGLEDA"
     gene            complement(1526..>2484)
                     /locus_tag="PKY13_08195"
     CDS             complement(1526..>2484)
                     /locus_tag="PKY13_08195"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOT79953.1"
                     /translation="SDFWGTKRVLVTGGNGFLGTALRAELESRRPDAIVTPSSAEFDL
                     RNPTDVDKMLTTHAPDIVVHLAARVGGIGANMERPADLYLDNLLMGTYVIDAARRLGI
                     AKTVVIGTICSYPKFTPVPFSETSLWQGYPEETNAPYGVAKLVQLVQVQSNRAQYGQS
                     AIYLMPTNLYGPGDKFNSSVSHVIPAIIKKCVDAKEAGADHIDVWGTGSASREFLYVD
                     DAARGIVAATEFYDGEDPVNLGTDEERPIRELVPLIAELVGFEGEIRWDPSKPDGQPR
                     RNVDTSRAAELFGFRAQIPFEEGLRRTIDWYLSHRAEAETRT"
     assembly_gap    2485..2584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2585..3770
                     /locus_tag="PKY13_08200"
     CDS             <2585..3770
                     /locus_tag="PKY13_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT79954.1"
                     /translation="GGGPARWAVGCRRRPGPVGLCQTPAMGEPVRVAYTVMQCWHRVP
                     GGTASSVLSLASALGDRDDVDLVGVGPWFGGVPAAPWVPPMPVRRLPVPYQLAYEAWY
                     RSSLLAPTWVVPDADVVHATTVTVPAKGRARGLVVTVHDLFPLTSPEQFTPRGVRIMT
                     GGIRAAIDRADLVCCPSQDTMDDCLRAGFAADRLRLVPWGATAHPVTDVDRRRVRERY
                     RLDRPFVLWVGTIEPRKNLPTLLEAFRRIQPTDVDLVLVGPVGWHEQLEGHIEGIGSK
                     VRQLGFVPAEDLPALYAEARLFCFPSTREGFGLPALEAMAQGTPVIAGAGTAVAEVVG
                     DGGVTIAPLDVDAWSEVLVGLLDDEPRRSALGAAALVRAGEFTWERCAEQMAAAYHEV
                     GG"
     gene            3767..4945
                     /locus_tag="PKY13_08205"
     CDS             3767..4945
                     /locus_tag="PKY13_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT79955.1"
                     /translation="MTRRVGVNLAWLVPGVVGGSEEATTRALGAVGDLAVAAGIEVVV
                     FGLPSLTDAHPALVERFEFHPVGAAGGSKVRRVAAETVDLPRLVARAGVDMVHHAGGV
                     IPLTARGRVTLAVHDTQPLDLPDNFSAVKRRYLGAMVPSSVRRADLITVPSRFVLGRL
                     VDGLGADPDRIAVVPWSVPRVVAPAAGAGAEVRRRHGLGDRRFVLYPAIAYLHKNHAV
                     LLDAVAAMGRDGGAPVDLVLTGAAGPLDAEVAERAAAPDLAGRVHVLGRVPAGDLAVL
                     LAEADAVAVPSRYEGFGLPALEALAAGRPTVVADAGSLPEVVDGAALVVDPDDVEGWA
                     AALRIAVDDPAERARLVDAGLAVAASGTPERTATALIDVWLRCLGGDETPPRTVRNPS
                     "
     gene            4942..6171
                     /locus_tag="PKY13_08210"
     CDS             4942..6171
                     /locus_tag="PKY13_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT79956.1"
                     /translation="MNLLVICPHYAPDVAPTGEVMTSIVEALAARGHRLHIVTALPWY
                     RHHRIDEGWDHRLVQTADTPWGAITRLHPFPTDKSNIPARALAFGGFTGVATAVSLRP
                     GLKPDAVLVMSPPLILGIAGWLAARRWRVPMVFNIQDVFPDVAIEVGAIKNPTVIKAA
                     RWLERFLYRRSDAVTVLSEDLRLNLAGKLAGHRPERVRVIPNFVDTDRIRPASRVNAY
                     RDELGLGDRTVVMYAGNIGYSQSVELMVGAARRFADRDDVVFVVNGSGSGRASVVESA
                     EGLDNIIFVGLQPKERLSEVLAAGDIHTVLLRRGLARSSVPSKMYSILASGRPCVASV
                     DDGTEVARSLAAADGGVAVPPEDVDAFCAALQPLLDDAAERERLGANGRRWVEAWASP
                     EAVAESYEALFDELLAR"
     gene            6230..7030
                     /locus_tag="PKY13_08215"
     CDS             6230..7030
                     /locus_tag="PKY13_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79957.1"
                     /translation="MATPSTSKKIQRVQQSGVTRRVGQRRPLGFPAAVVAIIVVGLVL
                     VWFARDARINVDGDRPRANQDKWYEAYGLYICDSYQANPPAPAESNDISTRGNGLIDV
                     FPFSDATAGDNATLGKFFSSIGLKVTTDGITTADGTKYEAGDECGVGKKKTDKTVVKL
                     FVWPPQASDKTKPEVITDDFGDVRIDEDKGAYALALVPESTDQIDLPGSVSNLADPEA
                     SVPATTSAPATTVPGETTVAPETTAAPAETTVAPTTTAPKATTTTAKG"
     gene            7033..8043
                     /locus_tag="PKY13_08220"
     CDS             7033..8043
                     /locus_tag="PKY13_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOT79958.1"
                     /translation="MRAVVLVGGFGTRLRPLTLDTPKQMLPVVDVTMFERVIARLGAC
                     GVDEAVVSLGFRPDRFVEAFPDGTCAGVALHYAIESEPLDTAGAIRFAATHAGVDDTF
                     IVVNGDVLTDLDYAWLVDEHRRLGAEATLHLIAVDDPSRFGVVATDDAGRVLEFVEKP
                     APGTEPTNLINAGTYVMEPSVIDRIADGRKVSVERETFPALAAAGTLFAVSTDDYWID
                     AGTPAAYLQANLDLIDGSRAEERAIDPAAQIDETATITRSVIGEGAVVGPDAVVADSM
                     VMAGAHLGAGVVVSGSIIGRSAQIGDRSAVTAGSVVGHDVVLAADTELTGATSPDPST
                     WQ"
     gene            8104..9054
                     /locus_tag="PKY13_08225"
     CDS             8104..9054
                     /locus_tag="PKY13_08225"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT79959.1"
                     /translation="MRALVTGGAGFIGSTLVDRLLAEGHAVDVIDNLSSGKLANLADA
                     RANRDHDVTFHQIDIREPGVVDLIERRSPDVVFHLAAQADVRVSVADPVLDASINVIG
                     ALNVLEGTRRAGADKIVFASSGGTIYGEPDVSSLPVEETHPLRPESPYGITKRVFTDY
                     LTAYRLLHDLEFTSLALGNVYGPRQDPHGEAGVVSIFAGRLLAGEPCTIFGDGQQTRD
                     FVFVDDVVDAFVRAATRGGGLLFNIGTGVEVSVNELYTSMAAAAGVTEPAVIAPARAG
                     ELNRIALSAAKAKIHLGWESWTTLDEGTKAVIDWTRETSS"
     gene            8991..>9665
                     /locus_tag="PKY13_08230"
     CDS             8991..>9665
                     /locus_tag="PKY13_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79960.1"
                     /translation="MDHARRGHEGRHRLDPRDLVLIEDIVTIRPGIPQLVAGVASLAI
                     WTAAVVALLVSRPLGGGWIVVAFTLFLGPQVAYCLASTARVVPRGIQVRNGLRRRFVD
                     FDEVAELRVTRVAYSFAARCPVKTEGLQRLRLDVVTTPGVVFPMVASERLVAVTHAQQ
                     TLAVGKQMHIRAPVRLEMEKDRAVRRDNLSGLTRQGRAHIVPVLRHAEQQAQLVIGKG
                     NARGRAA"
BASE COUNT         1354 a         3346 c         3320 g         1545 t
ORIGIN      
        1 tacgacttcc ggctgctcaa gggtgtggtc gacgtcaaca acgagcagtt cgagcgggtc
       61 gtccgcaagg ttgtcgccgc cgtcggtggc gacgtctccg gtcgccacat ctcggcctgg
      121 ggcctcacct tcaaggcccg caccgacgac acccgcatgt cgccggcgat cgaggtcatc
      181 aagcgcctgc aggcgctcgg cgccgaggtg accgcgtacg acccggcgat ccgtatggac
      241 atcgagggca tcacggtcgc caacgacccg atgacggcct gcaagggctc cgagctgctc
      301 atgatcctga ccgagtggga cgagttcaag tgggtcgacc tcgacgaggt cgccgagcag
      361 atgaccgagc gccgcatcgt cgacacccgc aacctgctcg accgctcccg cgcccagcgc
      421 gccgggttcg agtaccaggg catcggtcga cgctgagacc ttcgtcgccg cgatgatcgt
      481 cgagtaggcc aagtggcctg cttgactcgg gcgggcactg tgagttgaga tcgtccggcg
      541 tgcccgacgg ggggcacaca cgacgatggt gagcgggtag tgacgtgaga gtggtgttga
      601 ccggcggggc gggcttcctc ggtttccatc tgagcgagat cctgttggga cgtggcgacg
      661 aggtcgtgtg cgtcgacaac ctgatcagcg gcacccagag caacgtcgac gcgctctcac
      721 ggcacgacgg cttcgagttc ctcctgcagg acgcgtcggt gccgtacgag gtggccggtc
      781 cggtcgacgc agtgatgcac ttcgcgtcgc ccgcgtcggt gcccgactac ctggccaacc
      841 cgctcgaaac ccttcgggtc ggcagcaacg gcacgttcaa cgccctcgac ctcgctcgtg
      901 cccacgatgc ccgattcctc gtggcgtcca cgagtgagat ctacggtgag ccgctcgtac
      961 acccccagcc cgagtcgtac tggggcaacg tgaaccccaa cggcccccgc agcgtctacg
     1021 acgaggcgaa gcggttcgcc gaggcggtga cggccgcgta ccggcgtacg tacggcacgg
     1081 acacctcgat cgttcggttc ttcaacacct acggcccccg gatgcgcccc gaggacggcc
     1141 gcgtcgtcac gaacttcatc gtgcaggcac tgctgggcaa accgctcacg atctacggcg
     1201 acggcagcca gacccggagc ttcggctacg tgggcgacat cgccgccggc acgatcgcct
     1261 tgctcgacag cggtcacgcc ggcccgatga acctcggcaa ccccgacgag ttcacgatga
     1321 tcgagctggc cgacctcgtg ctggaactca ccggctcgac gtccgacctc gcgttcgagc
     1381 cgctgcccga ggacgacccg acccagcgtc gtcccgacat ctcactggcc cgggacgtgc
     1441 tcaactggga gccgacgacg gcgctccgcg agggtcttgt gccgacgatc gcgtacctcc
     1501 gggaactgct gggcctcgag gacgcctagg tccgggtttc ggcctcggcg cggtgggaga
     1561 ggtaccagtc gatggtgcgt cgcaggcctt cttcgaaggg gatctgcgcc cggaagccga
     1621 acagctccgc ggcccgtgac gtgtcgacgt tgcgtcgcgg ttgcccgtcg ggcttcgacg
     1681 ggtcccagcg gatctcaccc tcgaacccga ccagctcggc gatcaggggc acgagttcgc
     1741 ggatcggtcg ttcctcgtcg gtgccgaggt tgaccggatc ctcgccgtcg tagaactcgg
     1801 tggcggcgac gatgccacgt gcggcgtcgt cgacgtagag gaactcgcga ctcgccgagc
     1861 cggtgcccca cacgtcgatg tggtcggccc ccgcctcctt ggcgtcgacg cacttcttga
     1921 tgatcgcggg gatcacgtgg ctgaccgagg agttgaactt gtcgccgggg ccgtacaggt
     1981 tggtcggcat gaggtagatg gcggactggc cgtactgggc gcggttggac tgcacctgca
     2041 cgagctggac cagcttggcg acgccgtagg gggcgttggt ctcctcgggg tagccctgcc
     2101 agagtgacgt ctcgctgaag gggaccgggg tgaacttggg gtaggagcag atcgtcccga
     2161 tgaccacggt cttcgcgatg ccgagccgac gggcggcgtc gatgacgtag gtgcccatca
     2221 gcaggttgtc gaggtacagg tcggccggac gttccatgtt ggcgccgatg ccgccgaccc
     2281 gagcggcgag atgcaccacg atgtcgggcg cgtgcgtggt gagcatcttg tcgacatcgg
     2341 tcgggttccg gaggtcgaac tcggcgctcg acggcgtgac gatcgcgtcg ggacgccgcg
     2401 actcgagctc ggcgcggagc gcggtgccga gaaagccgtt gccgcccgtg accagcacgc
     2461 gcttggtgcc ccagaagtcg ctcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnncggagg cggcccggcc cggtgggcgg tcgggtgccg gaggcggccc ggcccggtgg
     2641 ggctgtgcca gactccggcg atgggtgagc cggtccgcgt cgcgtacacg gtcatgcagt
     2701 gctggcaccg ggtgccgggc gggacggcca gctcggtgct ctcgctggcg tcggcgctcg
     2761 gcgaccgtga cgatgtcgat ctcgtcggcg tcggcccctg gttcggcgga gtcccggcgg
     2821 caccgtgggt gcccccgatg ccggtgcggc ggctgccggt gccgtaccag ctcgcctatg
     2881 aggcgtggta ccgctcgtcg ctgctcgccc cgacgtgggt ggtgcccgat gctgacgtgg
     2941 tgcacgcgac gaccgtcacg gttcccgcca agggccgcgc tcgcgggttg gtcgtgacgg
     3001 tgcacgacct gtttcccctc acgtcgcccg agcagttcac cccccgtggt gtccggatca
     3061 tgacgggagg gatccgtgcg gccatcgacc gggcggacct cgtctgttgt cccagccagg
     3121 acacgatgga cgactgtctc cgggccgggt tcgccgctga ccgactgcgg ctcgtgccgt
     3181 ggggcgccac cgcccatccg gtcaccgacg tcgaccgccg ccgcgtccgc gagcggtatc
     3241 gactggaccg accgttcgtg ctgtgggtcg gcacgatcga gccgcggaag aacctgccga
     3301 ccctgctcga ggcattccgt cggatccaac caaccgacgt cgacctcgtc ctggtgggcc
     3361 cggtgggctg gcacgagcag ctcgagggcc acatcgaggg gatcggcagc aaggttcgcc
     3421 agctgggctt cgtgccggcc gaggacctgc cggcgttgta cgccgaggcc cgcctgttct
     3481 gctttccgag cacccgtgag gggttcggtc tgccggcgct cgaggccatg gcgcagggca
     3541 cgccggtgat cgccggtgcc ggcaccgcgg tcgcggaggt ggtgggcgat ggcggcgtca
     3601 cgatcgctcc gctcgacgtc gatgcctggt ccgaggtgct ggtgggactg ctggacgacg
     3661 agccgcggcg gtcggcactc ggcgcggccg cgctggtgcg cgccggcgag ttcacgtggg
     3721 agcggtgcgc ggagcagatg gcggccgcct atcacgaggt cggtgggtga cccgccgggt
     3781 cggcgtgaac ctggcctggc tggtgcccgg cgtggtcggt gggagcgagg aagcgacgac
     3841 gcgggcgctc ggagccgtcg gcgacctcgc cgtggccgca ggcatcgagg tcgtcgtctt
     3901 cgggctgccg tcgctcaccg acgcccaccc tgcgctcgtc gaacgcttcg agttccaccc
     3961 cgtcggcgcg gccggaggca gcaaggtgcg ccgcgtcgct gccgagaccg tcgacctccc
     4021 tcgcctcgtc gcccgggccg gcgtcgacat ggtgcaccat gccggtggtg tgatcccgct
     4081 gacggcgaga gggcgcgtga ccctcgccgt gcacgacacc caacccctcg atctgcccga
     4141 caacttctcg gcagtgaagc gccggtatct cggtgcgatg gtgccgagct cggtccggcg
     4201 cgccgatctc atcaccgtgc cgtcccgatt cgtgctgggt cggctggtcg acgggctggg
     4261 ggccgacccc gatcggatcg ccgtcgtgcc ctggtcggtc cctcgggtcg tggcgccggc
     4321 agccggcgcc ggcgccgagg ttcgccgccg tcacggtctg ggagaccgtc ggttcgtcct
     4381 gtacccagcc attgcctatc tccacaagaa ccacgccgtg ctgctcgacg ccgtcgcagc
     4441 gatgggtcgt gacggtggag cgcccgtgga cctggtgctc accggtgccg ccggcccgct
     4501 cgacgccgag gtggccgagc gtgccgctgc tcccgatctt gcgggccggg tccacgtgct
     4561 gggtcgggtg ccggccggcg acctcgcggt cctgctcgcc gaggcggatg cggtcgcagt
     4621 gccgtcgcgc tacgagggct tcgggctccc cgccctggag gcgctcgcgg cgggtcgtcc
     4681 cactgtcgtc gccgacgccg gctcgttgcc cgaggtcgtc gacggtgcgg cgctcgtcgt
     4741 cgatcccgac gatgtcgagg gatgggctgc tgcgctccgg atcgccgtcg acgaccccgc
     4801 cgaacgggct cgacttgtcg acgccgggct cgccgtcgca gcatctggga cgcccgagcg
     4861 cactgcgacg gccctgatcg atgtctggct tcgttgcctc ggcggcgacg agacacctcc
     4921 ccgaaccgtc cggaacccgt cgtgaatctc ctcgtcatct gtccccacta cgcccccgac
     4981 gtcgccccca cgggcgaggt catgacctcg atcgtggagg cgctcgccgc tcggggtcat
     5041 cgactccaca tcgtcacggc actgccgtgg taccggcacc accgcatcga cgaggggtgg
     5101 gaccaccggc tggtccagac ggccgacacg ccctgggggg cgatcacccg cctccacccg
     5161 ttcccgaccg acaagtcgaa cattccggcc cgcgccctcg cgttcggggg cttcaccggt
     5221 gtggcgacgg ccgtctccct ccggccggga ctgaagcccg atgccgtcct ggtcatgtcg
     5281 ccgccgctga tcctcgggat cgccggctgg ctggccgccc gtcgctggcg ggtgcccatg
     5341 gtcttcaaca tccaggacgt gttccccgac gtcgccatcg aggtcggggc catcaagaac
     5401 cccaccgtca tcaaggcggc gcggtggctc gagcggttcc tgtaccgccg ctccgacgcg
     5461 gtgacggtgc tcagcgagga cctccggctg aacctcgccg ggaagctcgc cggccaccgt
     5521 ccggaacggg tccgggtcat tccgaacttc gtcgacaccg atcgaatccg tcccgcgtcg
     5581 cgggtcaacg cgtatcgcga cgagctcggt ctcggtgacc ggaccgtggt gatgtacgcc
     5641 ggcaacatcg gctactcgca atcggtcgaa ctgatggtgg gtgcggcccg gcgattcgcg
     5701 gaccgtgacg atgtggtgtt cgtcgtcaac ggttcgggct cggggcgcgc ctcggtggtc
     5761 gagtcggcgg aggggttgga caacatcatc ttcgtcgggc tccagccgaa ggaacgcctg
     5821 tcggaggtgt tggccgctgg agacatccac acggtgctcc tccgccgcgg gctcgcccgg
     5881 tcgagcgtcc cgtcgaagat gtactcgatc ctggcgtcgg gtcgcccctg tgtggcgtcg
     5941 gtcgacgacg gcaccgaggt cgcccgctcg ttggccgccg ccgacggcgg tgtggccgtg
     6001 ccgcctgagg atgtcgacgc cttctgcgcc gccctgcagc cgctgctcga cgacgccgcc
     6061 gaacgcgagc gcctcggcgc gaacgggcgt cgatgggtcg aggcgtgggc atcgcccgaa
     6121 gccgtcgccg agtcgtacga ggcgttgttc gacgagctgc tggcccgctg atcgactgct
     6181 gcttgcgggt tcacgagtcg gcgtgtccgt cggctaccgt ggtcccgcta tggctacgcc
     6241 ttccacgtcc aagaagatcc agcgcgtcca gcaatccggt gtgacgcgcc gggtcggcca
     6301 gcgccgaccg ctcgggttcc ccgccgccgt cgtcgcgatc atcgtcgtcg ggctcgtgct
     6361 cgtgtggttc gcccgcgacg cccgaatcaa cgtcgacggc gaccgccccc gcgccaacca
     6421 ggacaagtgg tacgaggcgt acggcctcta catctgcgac tcctaccagg ccaacccgcc
     6481 ggcccccgcc gagtcgaacg acatctcgac gcggggcaac ggcctcatcg acgtgttccc
     6541 gttctccgac gcgacggcag gcgacaacgc cacgctcggc aagttcttct cgtcgatcgg
     6601 gttgaaggtg accaccgacg gaatcaccac cgccgacggc acgaagtacg aggccggtga
     6661 cgagtgcggc gtcggcaaga agaagaccga caagaccgtg gtgaagttgt tcgtgtggcc
     6721 gccgcaggcg tccgacaaga ccaagcccga ggtcatcacc gacgacttcg gcgacgtccg
     6781 catcgatgag gacaagggcg cgtacgcctt ggcgctcgtc cccgagtcga ccgaccagat
     6841 cgaccttccc ggttcggtct cgaacctcgc tgatcccgag gcgtcggtcc cggccacgac
     6901 gtcggcaccg gcgaccaccg tccccggcga gaccacggtc gcgcccgaga ccaccgccgc
     6961 cccggcggag acgaccgtgg caccgaccac gacggccccg aaggcgacga ccacgaccgc
     7021 gaagggctga gcgtgcgcgc cgtcgtgctg gtcgggggct tcggcacccg cctccgaccg
     7081 ctcacgctcg atacgccgaa gcagatgctg cccgtcgtcg acgtgacgat gttcgagcgc
     7141 gtcatcgccc gcctcggggc ctgcggcgtc gacgaggctg tggtgtcgct cgggttccgc
     7201 cccgaccggt tcgtcgaggc cttccccgat ggcacgtgtg ccggtgtcgc gctgcactat
     7261 gcgatcgagt ccgaaccgct cgacacggcc ggcgccatcc gcttcgccgc cacccacgcc
     7321 ggggtcgacg acacgttcat cgtcgtcaac ggcgacgtgc tgaccgatct cgactacgcc
     7381 tggctcgtcg acgaacatcg tcgcctcggg gccgaggcca cgctccacct gatcgccgtc
     7441 gacgatccgt cgcggttcgg cgtcgtggcc accgatgacg ccgggcgggt gctcgagttc
     7501 gtcgagaagc cggcgccggg caccgagccg accaacctga tcaacgccgg tacctacgtg
     7561 atggagccgt cggtcatcga ccgcatcgcc gacggccgca aggtgtccgt cgagcgcgag
     7621 acgttcccgg cccttgccgc cgccggcacg ctcttcgccg tgtcgaccga cgactactgg
     7681 atcgacgccg gcaccccagc ggcctacctg caggccaacc tcgacctgat cgacggttcc
     7741 cgggccgagg aacgcgctat cgaccctgca gcgcagatcg acgagacggc gacgatcaca
     7801 cggtccgtca tcggcgaggg cgccgtcgtc ggacccgatg ccgtcgtggc cgactcgatg
     7861 gtcatggccg gggcacacct gggggcgggc gttgtggttt cgggctcgat catcggccga
     7921 tcggcccaga tcggtgaccg cagcgcggtg accgccggca gcgtggtggg ccacgacgtg
     7981 gtgctcgccg ccgacaccga actgaccggc gcgacgtcgc cggatccgtc cacctggcaa
     8041 tgacgccagc acgatcaggg cggagccggg cggggagtcc gtccgccgac gaaagggatt
     8101 gatatgcgag cactcgtgac cggcggagca gggttcatcg gttcgacgtt ggtcgaccgg
     8161 ttgctcgccg agggccacgc tgtcgacgtc atcgacaacc tgtcctcggg caagttggcc
     8221 aatctcgccg acgcccgcgc caaccgcgac cacgatgtca cgttccacca gatcgacatc
     8281 cgggagccgg gtgtggtcga tctgatcgag cggcgctcac ccgacgtcgt gttccacctc
     8341 gccgcccagg ccgacgtgcg ggtctcggtc gccgacccgg tgctcgatgc gtcgatcaac
     8401 gtcatcgggg cactgaacgt tctcgagggc acacggcgcg ccggcgccga caagatcgtg
     8461 ttcgcctcca gcggcggcac gatctacggc gagcccgacg tgtcgagcct gccggtcgag
     8521 gagacccacc cgctgcgtcc ggagtcgccg tacggcatca ccaagcgggt gttcaccgac
     8581 tacctcacgg cgtaccggct cctgcacgat ctcgagttca cgtcgctcgc cctgggcaac
     8641 gtctacgggc cccgccagga tccccacggt gaagccggtg tggtgtcgat cttcgccggc
     8701 cggctcctcg ccggggagcc ctgcacgatc ttcggtgatg gtcagcagac ccgcgacttc
     8761 gtgttcgtcg acgacgtggt cgacgcgttc gttcgcgccg ccacccgtgg cggtggcctg
     8821 ctgttcaaca tcggcacggg cgtcgaggtg tcggtcaacg agctgtacac gtccatggct
     8881 gcggccgccg gcgtgaccga gccggcggtc atcgctcccg cccgcgccgg cgagctcaac
     8941 cggatcgccc tcagcgctgc gaaggccaag atccatctcg gctgggagtc gtggaccacg
     9001 ctcgacgagg gcacgaaggc cgtcatcgac tggacccgcg agacctcgtc ctgatcgagg
     9061 acatcgtcac gatccggccg ggaatcccgc aactggtcgc gggcgtcgcg tcccttgcaa
     9121 tatggaccgc tgccgtcgtt gcgcttctcg tctctcgccc gctcggaggc ggttggatcg
     9181 tcgtcgcgtt cacgctcttc ctcggtcccc aggtggcgta ctgcctcgcg tcgaccgcgc
     9241 gtgtcgtacc ccggggcatc caggtccgca acgggctgcg gcggcgcttc gtggacttcg
     9301 acgaggtcgc cgagctcagg gtcacccggg tggcgtacag cttcgccgct cgctgcccgg
     9361 tgaagacgga gggattgcag cggctccgcc tcgatgtcgt gaccaccccg ggtgtcgtct
     9421 tcccgatggt ggcaagtgaa cggctcgttg cggtcacgca cgcgcaacag acacttgctg
     9481 tcggaaagca aatgcacatt cgggctccag tccgcctcga aatggagaaa gaccgtgccg
     9541 tccggcgcga caacctgagt ggcctgacac ggcaagggcg ggctcacata gttccggtcc
     9601 ttcggcacgc cgagcagcag gcgcaacttg tcatcggcaa gggcaacgcc cgtggccgtg
     9661 ccgcc
//
LOCUS       DAOSOA010000102         9561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7117_length_9561_cov_5.099726, whole genome shotgun sequence.
ACCESSION   DAOSOA010000102 DAOSOA010000000
VERSION     DAOSOA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7117_length_9561_cov_5.099726"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKY13_08235"
     CDS             complement(<1..286)
                     /locus_tag="PKY13_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT79961.1"
                     /translation="MEFGLFLNGYLPGPAAHDPASEHEMIMREMSYAIHADKFNWKYA
                     WLGEHHALTEYSHMSAPEVVIPYVAAKTERIHLGSAIINLSPPVNQPTRNA"
     gene            405..716
                     /locus_tag="PKY13_08240"
     CDS             405..716
                     /locus_tag="PKY13_08240"
                     /inference="COORDINATES: protein motif:HMM:NF021552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HOT79962.1"
                     /translation="MCRNITTLRGLEPAATPTEIEAAARQYVRKVTGVQKTAPATEEA
                     FERAVALIAEATADVIAALPPRRNPPPTLPPLRRIKAAGVSPGSAPHPRARGGARSGT
                     I"
     gene            complement(798..2537)
                     /locus_tag="PKY13_08245"
     CDS             complement(798..2537)
                     /locus_tag="PKY13_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOT79963.1"
                     /translation="MTGVFDVSKGDLVGLVIGAGGAGATAAVATGTATASVTLDEIAT
                     MEAEHGPRWVVWSQATTGALVAAGIRVARAWDLVAVHRLLYGGWSSDPGRIWARLNGL
                     STANLPQVRPPDLFNQVDPAAAGNDPIDDDGYVRAEWAAGAWAESPERAAAWARLAWR
                     TAQLQQGWLDASSDPARMTATARSESAAELLCAELAHDGLPLDRERAEAIIESFVGRR
                     PRSDADAAVLRAERDELVLRHAPPDSRFDLRSPGQVKSLLRRIGIEVTDTRAWRLESL
                     RDEHPLVEALLAWRKAERVATTYGYAWLDEYVGDDPETGAGRLRGEWSGSDGAAGRMT
                     ASAGLHNLPGDMRGAVVADDGWVFVRADLGQIEPRVLAAVSGDVALARASADDDMYTP
                     VARQLGVDRATAKVAVLGAMYGQTTGHGAQALRGLQQNYPVAIEYLEAASRTAQVGTD
                     LRTYGGRLVRMGTANANDVSERDLRSHAAARGRYGRNAMVQGAAAEFFKLWAVTVRAR
                     VAPLGARIVLCLHDELLVHTPLESAEATARLVDECLQEAAHRWAPRPVAGAPPVRFLT
                     DLSVVGCWADAKD"
     gene            2589..2801
                     /locus_tag="PKY13_08250"
     CDS             2589..2801
                     /locus_tag="PKY13_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HOT79964.1"
                     /translation="MAEKVKGPASYFPSIEAKYGRPIAEWKALIRSSDIEGHMKLVTW
                     LKAEHGLGHGHANALVADTLAEDRDS"
     gene            2814..3104
                     /locus_tag="PKY13_08255"
     CDS             2814..3104
                     /locus_tag="PKY13_08255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT79965.1"
                     /translation="MPVEVAHAPERSEFVALDETGQVGVLVYALADGVIDLQHTVVQP
                     AAGGRGIGSDLVVAALEFARAEGLRVIPTCPFVPAVIARHPDYADLVRPTPR"
     gene            3140..>3694
                     /gene="yihA"
                     /locus_tag="PKY13_08260"
     CDS             3140..>3694
                     /gene="yihA"
                     /locus_tag="PKY13_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOT79966.1"
                     /translation="MPPLPLRFVTSASTYETLPATPAELAFIGRSNVGKSSLINALAN
                     QKQLAKVSKTPGRTQLLNLFELRDGRTLVDLPGYGFANAPKAARANWGRMTERYLLER
                     EGLERILVLVDGEIGPTKLDVNMLEWLRDCDLPFAVIATKHDKVKSSVRQKRKRELAA
                     GCGLEERDITWVSASKGVNLDQLRD"
     assembly_gap    3695..3794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3795..4339)
                     /locus_tag="PKY13_08265"
     CDS             complement(<3795..4339)
                     /locus_tag="PKY13_08265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT79967.1"
                     /translation="MDRDPRSAAPSPVGGWNDGEQVAWYLERVGRLEARAAGESMLVD
                     LLPPAPRRVLDLGCGDGRLAALVLDVRPSVEAAVAVDRSEVMLAHARERFVDDDRVEV
                     RAWDLDDPIGPLGRFDVVVSGFAIHHLDDERKRALFAEIAAQLEPGGAFLNLEVVASG
                     SERRHADFLTAIGRTADDPEDR"
     gene            4375..4653
                     /locus_tag="PKY13_08270"
     CDS             4375..4653
                     /locus_tag="PKY13_08270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT79968.1"
                     /translation="MILIVDDADRSEYVATLDGVEAGVIVYERRDGAIDLEHTIVRPE
                     AEGHGVGSTLIRFALDAARTSGEPVIPSCPFVQAFLEKHPEYLDVVRP"
     gene            complement(4678..7272)
                     /locus_tag="PKY13_08275"
     CDS             complement(4678..7272)
                     /locus_tag="PKY13_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT79969.1"
                     /translation="MSAALARREIRSRPGRFAWLIVAVTVAVGFTVGAFGFSTQLTKL
                     LDPSATGADGLDALPRGAVVLTADTKAVTTATALDERLSATVEAIAGVALVEGSYDQP
                     IGFRLPAGSQPERPVILRGVVLSSTYSEQRWAIQVGRAPSGPDEVAVDLAGLVVGQTS
                     VGAVATIEFPTGARDVAVVGLLAPVGDTTPLPSARPDDASSIALAAGHAILDPGVAPI
                     LLDAAGRVDRITVTPQPGVDPGALERRIRAAVPPGVRVSSALSRSAVTQQTVERIDEG
                     VQQAVTVFAGLTVLISALVVTNTLGVLVAQRTREFGLLRLVGASRAQVLRTVMGESLG
                     VGLVGAVLGLGLGVVLAYAAALVVRDSTVPVGFELTSTMVLVAVGVGVLVTGVGGIVP
                     ALRAGRVTPLAALSDTRAGADRRGAAWVPLAAFAVGCAMAGAAATRPGGPDGAWIVVA
                     GVGALVAFVGVAGLSRWLVGPLLATVGAVLGPVSGRAGRLGVANARRSPGRTAGAAST
                     LMVGLALVGLVATIGASIRSTVESQFDTAGSADLYLERRGVVRVSTQALDVELAGRAR
                     RPFRYASITTVDGVVLGRAGATTPLSASFLPELAELVDLGITDGAIPGFADDPAAERV
                     SVMLSDSAAAELGLAVGDEALLRTISGSERTLVVAATYSNTAIAGDAVVDVTGVDPGT
                     LGTFELGVLRFEGRHGDRSVRAVERAAGHFPKVRVHTPAEFGALNASVTDTVLRIIAV
                     VLAGMIGIGYLGLVATLGLATLERRNELVMLRAIGAARAQVRAMVRIEAMLVGLVASV
                     VGLGVGVGLGWVAAISAPPDLVASVVVPWASIVAVGVVSVVLALTVSLGVARRASLVP
                     PAEAGRSV"
     gene            7329..8414
                     /locus_tag="PKY13_08280"
     CDS             7329..8414
                     /locus_tag="PKY13_08280"
                     /inference="COORDINATES: protein motif:HMM:NF040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79970.1"
                     /translation="MRQRVVVWGTGNMGGAAIRSATGFPGLDLVGVVTGTDSKAGQDA
                     AAFAGLDEPTGVLLTTDVEAALAACDAVAYMASGDTRPDEALADIERCLRAGANVVTP
                     SLYSLYDPSSAPAELRERLNDACEAGGASLLVSGVDPGWANDAMAVLAAGLCTRIEEI
                     RCQEIFDYSTYDQPHAVRVLCGFGQPMEEVPMMLLPSVPTMVWGGNIRLIGRGLGIEI
                     DEISEVVERRPLEASLDTEMGRFDAGTQGAFRLQIIGKVAGRSRVVIDHITRVDPACA
                     PEWPQPDEGVGDHRIIVDGDPKLTIVVRADVAGGTRADGGNATAANRLLGAISWLATA
                     TPGLYDGLDVPLNSPLEPSIAAERWSS"
     gene            8414..8935
                     /locus_tag="PKY13_08285"
     CDS             8414..8935
                     /locus_tag="PKY13_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79971.1"
                     /translation="MFLEIPEGQHPIFHVWGEMVPGIGPAASRFSQKVYEDTTLELET
                     FEAARLRMAQINGCLFCKDWRTERDGQTVGDDFPGFVEDWAGDGGAHLSARARIAAEY
                     AERFALDHHGIDDAAWARFRAEFADAELVELTMCLGSWLAFGRLNHLFGLDSACQLPT
                     MEEREAVTKAPGA"
     gene            complement(8974..9390)
                     /locus_tag="PKY13_08290"
     CDS             complement(8974..9390)
                     /locus_tag="PKY13_08290"
                     /inference="COORDINATES: protein motif:HMM:NF036804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HOT79972.1"
                     /translation="MAKPNQRTTAAACPCGSGEPYSRCCRPFHLRLAEPPTAEQLMRS
                     RYSAYAKGLDAYLLHSWDPAHRPATIELDPRHRWTGLTIIATSGGAPDDTAGTVEFVA
                     AFEIDRGAGPRPGELRENSSFGRHEGRWVYETEVDG"
BASE COUNT         1468 a         3535 c         3196 g         1262 t
ORIGIN      
        1 cggcattgcg ggtgggctgg ttcaccggcg gcgagaggtt gatgatcgcc gagccgaggt
       61 ggatgcgctc ggtcttggcg gccacgtagg ggatgaccac ctcgggcgcc gacatgtggg
      121 agtactcggt gagggcgtgg tgctcgccga gccacgcgta cttccagttg aacttgtcgg
      181 cgtggatggc gtagctcatc tcacgcatga tcatctcgtg ctccgacgcc gggtcgtgag
      241 cggcgggtcc gggaaggtac ccgttgagga agagaccgaa ctccatgaga tgcacccctg
      301 ttgtggaggg cccgaacgcc gggccacatt tcgagaacgc gttctaatgt aagccattag
      361 acggtgcccg gcaaccgttc gtgacgaggc gcgagactgg ccgcatgtgc cgcaacatca
      421 ccaccttgcg ggggctcgaa cccgccgcca caccgaccga gatcgaggcc gccgcccgtc
      481 agtacgtccg caaggtgacc ggggtgcaga agaccgcccc ggcgaccgag gaggccttcg
      541 aacgcgccgt cgccctcatc gccgaggcca cggcggacgt catcgccgcg ctcccgccgc
      601 gccgcaaccc gcctccgaca ttgccgccgc tccgccgtat caaggccgcg ggtgtgtcgc
      661 ccggcagcgc accacacccc cgtgcccgcg gcggtgcccg ctccggcacc atctgacccg
      721 ccctcggggt tttgtgctgc gaggagcgac gtcagcgtcg cttctcgcag cacaaaaccc
      781 ctgacgcggc tgaccgttca gtccttggcg tcggcccagc acccgacgac actcaggtcc
      841 gtgaggaagc gcacgggcgg cgcaccggcc accggccgcg gcgcccaacg gtgcgccgcc
      901 tcctggaggc actcgtcgac caaccgggcg gtcgcctccg ccgactccag cggagtgtgc
      961 acgagcagct cgtcgtggag gcacaacacg atgcgcgccc ccaggggcgc aacccgggcg
     1021 cggaccgtga cggcccacag cttgaagaac tccgccgccg cgccctggac catcgcgttt
     1081 cggccgtagc gcccccgcgc cgcagcgtgg ctgcggaggt cgcgctcgct cacgtcattg
     1141 gcgttggccg tgcccatccg caccagccgg ccgccgtagg tccgcagatc ggtcccgacc
     1201 tgcgccgttc gcgacgccgc ctccaggtac tcgatcgcca ccggatagtt ctgctgcagc
     1261 ccccgcaacg cctgggcgcc gtgccccgtc gtctgcccgt acatcgcgcc gagcacggcc
     1321 accttcgccg tcgcccggtc gacgccgagt tgccgggcca ccggggtgta catgtcgtcg
     1381 tcggcgctgg cccgggccag ggcgacgtcg ccggataccg cggcgaggac acgcggttcg
     1441 atctggccca ggtcggcgcg cacgaacacc cagccgtcgt cggcgacgac cgcgccccgc
     1501 atgtctcccg gcaggttgtg caggccggcc gacgccgtca tccgcccggc ggccccgtcg
     1561 ctccccgacc actcgccacg gagccgtccc gcgcccgtct ccggatcatc gcccacgtac
     1621 tcgtcgagcc atgcgtaccc gtacgtcgtg gccacacgtt cggccttccg ccaggccagt
     1681 agggcctcga ccagcgggtg ttcgtcgcgg agcgactcca accgccacgc ccgcgtgtcg
     1741 gtgacctcga tcccgatccg ccggagcagc gacttcacct ggcccgggct ccgcaggtcg
     1801 aaccggctgt cgggcggggc gtggcgaagc accagctcgt cacgctcggc acgcaacacc
     1861 gccgcgtcgg cgtccgaccg cggtcgacgg cccacgaacg actcgatgat cgcctcggcg
     1921 cgctcacggt cgagtgggag cccgtcgtgg gcgagttcgg cgcagagcag ctccgctgcc
     1981 gactccgagc gagcggtggc ggtcatgcgg gcgggatccg acgacgcgtc gagccatccc
     2041 tgctgcagct gtgcggttcg ccaggcgaga cgcgcccacg ccgcggcgcg ttcgggcgac
     2101 tccgcccacg ctccggcggc ccactcggcg cgtacgtagc cgtcgtcgtc gatcgggtcg
     2161 ttccccgccg ccgcgggatc gacctggttg aacaggtcgg gcggacgaac ctgcgggagg
     2221 ttcgcggtcg acaggccgtt caaccgcgcc cagattcgcc ccgggtcgga cgaccagccg
     2281 ccgtacagca atcggtggac ggcgaccagg tcccacgctc gtgccacgcg aatccccgcg
     2341 gccacgagcg ccccggtggt ggcctgcgac cagaccaccc atcgtggtcc gtgctcggcc
     2401 tccatcgtcg cgatctcgtc gagggtgaca ctcgccgttg ccgtgccggt ggccacggcg
     2461 gcggtcgcgc ccgcaccgcc cgcaccgatc accagaccga cgagatctcc cttcgacaca
     2521 tcgaacacac ccgtcaccct gccatcccca cgccgtcggg agtggcaccg ccggtacgct
     2581 cgggccccat ggcagagaag gtgaagggcc ccgcgtcgta cttcccgtcg atcgaggcca
     2641 agtacggccg gccgatcgcg gagtggaagg cgctgatccg gtcgagcgac atcgaggggc
     2701 acatgaagtt ggtcacctgg ctgaaggcgg agcacggcct cggccacggc cacgcgaacg
     2761 ccctggtcgc ggacaccctg gccgaggacc gcgacagctg attggatgga cgcatgcccg
     2821 tcgaggtcgc ccacgcccct gaacgttccg agttcgtcgc cctcgacgag accggccagg
     2881 tcggtgtact ggtgtacgcc ctcgcggacg gggtgatcga cctccagcac acggtggtgc
     2941 aacccgccgc tggaggtcgt ggtatcgggt cggatctcgt ggtcgccgcg ctcgagttcg
     3001 cccgcgccga gggcctgcga gtgatcccga cgtgcccatt cgtcccggcc gtcatcgccc
     3061 gccaccccga ctacgccgac ctggtccgac cgacgccccg ctgaccgtcc cgtcccccgg
     3121 ttgctgccat catgggcgga tgccgccact ccccctccgg ttcgtgacgt cggcgtcgac
     3181 gtacgagacg ctgcccgcca caccggccga gctggcgttc atcgggcggt ccaacgtcgg
     3241 caagtcgtca ctcatcaacg cgctggccaa ccagaagcag ttggccaagg tgtcgaagac
     3301 gccgggccgg acccaactcc tcaacctgtt cgagctccgt gacggccgca ccctcgtcga
     3361 cctgccgggc tacggcttcg ccaacgcccc caaagcggcc cgcgccaact ggggccgtat
     3421 gaccgagcgc tacctgctcg aacgtgaggg actcgaacgc atcctcgtgc tcgtcgacgg
     3481 cgagatcggg ccgaccaagc tcgacgtcaa catgttggag tggttgcgcg actgcgacct
     3541 gccgttcgcg gtgatcgcca ccaagcacga caaggtgaag tcgtccgtgc gtcagaagcg
     3601 caagcgggaa ctcgcggcgg gctgtggcct cgaagagcgc gacatcacgt gggtcagcgc
     3661 atcgaagggt gtgaacctcg accagctccg cgacnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnncgatcc tcggggtcgt cggccgtccg accgatcgcg gtgaggaagt
     3841 ccgcgtggcg gcgctccgat ccgctcgcca cgacctcgag gttcaggaac gcgcccccgg
     3901 gctcgagctg cgccgcgatc tcggcgaaga gcgcccgctt ccgctcgtcg tcgaggtggt
     3961 ggatcgcgaa tcccgagacg acgacgtcga acctaccgag cgggccgatg gggtcatcga
     4021 ggtcccatgc ccgcacttcg acgcggtcat cgtcgacgaa acgctcacga gcgtgggcga
     4081 gcatcacctc cgagcggtcg accgccaccg cggcctcgac gctcggccgg acgtcgagca
     4141 ccagcgcggc aagcctgccg tccccacaac ccagatcgag cacacgccgg ggcgccggcg
     4201 gcagcagatc cacgagcatc gactcgccgg cagcgcgtgc ctcgagccga ccgacgcgct
     4261 cgagatacca cgcgacctgc tcgccgtcgt tccaaccgcc gaccggtgac ggggcagcag
     4321 agcgggggtc cctgtccatg gcccgagtgt ggccgcgtgc catgatccat gtccatgatc
     4381 ctgatcgtcg acgacgccga ccgctccgag tacgtcgcca ccctcgacgg ggtcgaggcg
     4441 ggcgtcatcg tgtacgagcg ccgtgacgga gcgatcgacc tggagcacac gatcgtgcga
     4501 cccgaggccg aaggtcacgg ggtcggttcg acgctcatcc ggttcgcgct cgacgccgcc
     4561 cgcacgtcgg gcgagccggt gatcccgtcg tgcccgttcg tgcaggcgtt cctcgagaag
     4621 caccccgagt acctcgacgt cgtgcgcccc tgagggcatc tgcccgcggc tgcacgatca
     4681 gacactccga ccggcttccg ccggtggtac gagcgaggcc cgacgagcga cgcccagcga
     4741 caccgtcagc gcgagcacca ccgacaccac gccgaccgcg acgatcgacg cccacgggac
     4801 caccacgctc gccacgagat cgggcggcgc cgagatcgcg gcaacccaac cgaggccgac
     4861 gccgacgccg agtccgacga ccgacgcgac gaggccgacg agcatcgcct cgatccgcac
     4921 catcgccctg acctgcgctc gcgccgcgcc gatggcccgc agcatgacca gctcgttgcg
     4981 ccgctccagc gtcgcgaggc cgagtgtcgc gacgagcccc agatagccga tgccgatcat
     5041 gccggcgagc acgacggcga tgatccgcag cacggtgtcg gtcaccgagg cgttgagcgc
     5101 accgaactcg gcgggggtgt gcacccgcac cttcgggaag tgccccgcag cccgctcgac
     5161 ggcgcggacc gatcgatcgc cgtgccggcc ctcgaaccgg agcacgccga gctcgaacgt
     5221 tccgagcgtg ccggggtcga cgccggtgac atcgacgacg gcgtcgccgg cgatcgctgt
     5281 gttcgagtag gtcgctgcga cgacgagcgt ccgctctgaa cccgagatcg tgcggagcag
     5341 cgcctcgtcg ccgaccgcga ggccgagctc ggcggccgcc gagtccgaca acatgaccga
     5401 cacacgttcg gccgcggggt cgtcggcgaa accggggatc gccccgtcgg tgatgccgag
     5461 atcgacgagt tcggcgagtt cagggaggaa cgacgccgac aacggcgtcg tcgcgcccgc
     5521 acgtccgagg acgacgccgt cgacggtcgt gatcgacgcg tagcggaagg ggcgccgggc
     5581 ccgtcccgcg agctcgacat cgagggcctg ggtcgacaca cgaaccacac cgcgccgttc
     5641 caggtacaga tcggcgctgc cggccgtgtc gaactgcgac tcgaccgtcg accgaatcga
     5701 cgcgccgatg gtggccacga ggccgacgag cgcaaggccc accatcagcg tcgacgccgc
     5761 accggcggtc cggcccggag aacgccgggc attcgcgaca ccgagacggc cggcgcgccc
     5821 gctgaccggt ccgaggaccg cgccgacagt cgccaacaac ggcccgacca accatcgcga
     5881 cagccctgcg accccgacga acgccacgag cgcaccgaca ccggcgacga cgatccacgc
     5941 tccgtcgggc ccgcccggcc gggtcgcggc agcccctgcc atcgcacacc ccacggcgaa
     6001 cgcggcgagt ggtacccacg cggcgccccg gcggtcggca ccggcacggg tgtcggacag
     6061 cgctgcgagt ggcgtcaccc gaccggcgcg cagcgcgggc acgatgccgc cgacgccggt
     6121 cacgagcacc ccgacgccga cggcgacgag caccatcgtc gaggtcagtt cgaagccgac
     6181 gggcacggtg ctgtcgcgca cgacgagcgc cgccgcgtac gcgagcacga cgccgagccc
     6241 aaggccgagg accgcaccga cgaggccgac gccgagcgac tcccccatca cagtccgcaa
     6301 cacctgcgcc cgcgacgctc cgaccagtcg caacaacccg aactcccggg tgcgttgcgc
     6361 cacgagcacg cccagggtgt tggtcacgac cagcgcgctg atgaggaccg tcaggccggc
     6421 gaagaccgtc accgcctgct ggaccccctc gtcgatccgc tcgacggtct gctgggtcac
     6481 cgccgatcgc gacagggccg agctgacccg aacaccgggc ggcaccgcgg cccgaatccg
     6541 ccgctccagc gctccgggat cgacgcccgg ttgtggggtc acggtgatgc ggtcgacgcg
     6601 cccggcagcg tcgaggagga tcggggcgac gccgggatcg aggatcgcat gccccgcggc
     6661 caacgcgatc gacgacgcat cgtcggggcg ggccgacggg agcggcgtgg tgtcgccgac
     6721 cggcgcgagc agacccacga ccgccacgtc gcgggcgccg gtcgggaact cgatcgtcgc
     6781 aaccgcaccg accgacgtct ggccgacgac gaggccggcg aggtcgacgg ccacctcgtc
     6841 gggtccactg ggcgctcgtc ccacctggat cgcccagcgc tgttcggagt aggtcgatga
     6901 caggacgacg ccgcgcagga tcaccgggcg ctccggttgt gatccggccg gcaggcggaa
     6961 cccgatcggc tggtcgtagg agccctcgac gagcgccacg ccggcaatcg cctcgacggt
     7021 ggccgacaac cgctcgtcga gcgctgtcgc cgtggtcacc gccttcgtgt cggccgtgag
     7081 cacgaccgca ccccgtggaa gcgcgtccaa gccgtcggcg ccggtcgccg acgggtcgag
     7141 caacttcgtc agctgcgtcg agaacccgaa cgccccgacg gtgaacccca ccgcgacggt
     7201 cacggccacg atcagccagg cgaaccttcc cgggcgggac cggatctccc gacgagcgag
     7261 cgctgccgac atcgggcgca cgctacggca ggacaccccg agccccagcg cgggggtagg
     7321 ttggcccgat gcgacaacgt gtggtggtgt ggggaaccgg caacatgggt ggcgcagcca
     7381 tccgctcggc gacgggcttc cccggtctcg atctcgtcgg tgtcgtcacc ggcaccgact
     7441 ccaaggccgg acaggacgcc gctgcgttcg ccggtctcga cgaaccgacc ggggtgctgc
     7501 tgacgaccga cgtcgaagcc gccctcgccg cgtgcgacgc ggtcgcctac atggcgtcgg
     7561 gcgacacccg ccccgacgag gccctcgccg acatcgagcg gtgcctccgc gccggcgcca
     7621 acgtcgtgac cccatcgctc tactcgctct acgacccgtc gtccgctccc gccgagctcc
     7681 gggaacgcct caacgacgcc tgtgaggcgg gcggcgcatc gctgctcgtg agcggcgtcg
     7741 accccggctg ggcgaacgat gcgatggcgg tgctcgcggc cggtctctgc acccgcatcg
     7801 aggagatccg ttgccaggag atcttcgact actcgaccta cgaccagccg cacgccgtgc
     7861 gagtcctgtg cggcttcggg caacccatgg aggaggtgcc gatgatgctg ctgccgtcgg
     7921 tgccgacgat ggtgtggggc ggcaacatcc gactcatcgg ccgtgggctc gggatcgaga
     7981 tcgacgagat ctccgaggtc gtcgaacgtc gcccgctcga agcgtcgctc gacaccgaga
     8041 tgggccggtt cgacgccggc acccaggggg cgttccgcct ccagatcatc ggcaaggtcg
     8101 ccggtaggtc ccgagtcgtg atcgaccaca tcacccgtgt cgacccggcg tgcgcaccgg
     8161 aatggccaca acccgacgag ggcgtgggcg accatcgcat catcgtcgac ggcgacccca
     8221 agctcacgat cgtcgtgcgc gccgacgtcg ccggtggcac acgagccgac ggcggcaacg
     8281 ccaccgcggc caaccggctg ctcggcgcca tctcgtggct cgccaccgcg acccccggcc
     8341 tgtacgacgg cctcgacgtg ccgctcaact cgccgctcga accctccatc gccgctgaaa
     8401 ggtggtcctc ctgatgttcc tcgagatccc cgagggtcaa cacccgatct tccacgtgtg
     8461 gggcgagatg gtgcccggca tcggcccggc agcgtcccgc ttctcccaga aggtctacga
     8521 ggacacgaca ctcgagctcg agacgttcga ggccgcccgt ttgcggatgg cccagatcaa
     8581 cgggtgcctg ttctgcaagg actggcgcac cgagcgcgac gggcagaccg tcggcgacga
     8641 cttccccgga ttcgtcgagg actgggctgg cgacggtggc gctcacctgt cggcgcgcgc
     8701 ccgcatcgcc gcggagtacg ccgaacggtt cgccctcgac caccacggca tcgacgacgc
     8761 tgcgtgggcc cgcttccgtg ccgagttcgc tgatgccgaa ctcgtcgagc tgaccatgtg
     8821 tctcggttcc tggttggcgt tcggtcgcct caaccacctg ttcggcctcg actcggcgtg
     8881 ccagctcccc acgatggagg aacgcgaggc cgtcaccaag gcgcccggcg cctgacgtca
     8941 ccgatcggag cgggtccgac cggggtaccc aactcagccg tcgacctcgg tctcgtagac
     9001 ccagcggccc tcgtgccggc cgaacgagct gttctcgcgc agctcgcccg gacgcgggcc
     9061 cgcaccccgg tcgatctcga acgcggccac gaactcgacg gtcccggcgg tgtcgtcggg
     9121 ggcaccgccg gacgtcgcga tgatcgtcag tcccgtccac cggtgccgtg gatcgagctc
     9181 gatcgtcgcc ggacggtggg cgggatccca cgagtgcagc agatacgcgt cgagaccctt
     9241 ggcatacgcg ctgtagcggg accgcatcag ctgctcggcg gtcggcggct ccgccaaccg
     9301 caggtggaac ggccgacagc accgcgagta cggctcgccg ctcccgcacg ggcacgctgc
     9361 ggcagtcgtt cgctgattcg gcttggccat ggcggccgtc acgctagcgg cgccatcggc
     9421 gacgcaattc gtgccagaag tgacgcgacg cggcacatcg ggcacggatt gcgtggcgcc
     9481 accggtctca ggcgtcgacc tcgcccgcca ccgccgcggc caggtgttcg gccgtcagcg
     9541 ggtggcccgt cgctcgttcg a
//
LOCUS       DAOSOA010000103         9450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7220_length_9450_cov_7.445343, whole genome shotgun sequence.
ACCESSION   DAOSOA010000103 DAOSOA010000000
VERSION     DAOSOA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7220_length_9450_cov_7.445343"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1118
                     /locus_tag="PKY13_08295"
     CDS             261..1118
                     /locus_tag="PKY13_08295"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOT79973.1"
                     /translation="MLLTRRSKRTEPETEPEAPAKSPLDEAIERLGPLNAVAVQVLRS
                     IDHPMSTGATVAAAIELDPVLTSQVLRVANSASFGVLSTVSTTVRAVAVVGMPGVRSI
                     AAVSTVQRGAEMPPGFWEHSAATAAGCVAASSAIGVSKDEAFSMGLLHDLGWAILDVL
                     DPDTHRSMTDVLDDATLCAAEELAFGASHAQIAGRLLESWNFPATIVEAIGAHHLALD
                     DHRSTVLSPHTQVLLIGDALAHRLLAGAGYPFAGVDLGEYGFSEPAIEALLELTDEYM
                     RDIVQVMPR"
     gene            complement(1330..2523)
                     /locus_tag="PKY13_08300"
     CDS             complement(1330..2523)
                     /locus_tag="PKY13_08300"
                     /inference="COORDINATES: protein motif:HMM:NF023224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2399 domain-containing protein"
                     /protein_id="HOT79974.1"
                     /translation="MRVPQSLTTDELAMLWTRVRDRLEHNGDEWKGRVGVPALSAEGR
                     LTLESLVGRRLKSQVALGDLEAGLVRLGVGESLTDAIDRLGHPAVGDVAARRAARASA
                     AGAREQARSIVTTWPEPWAGEWIDGVIRAGVLAGLDAPDATRLVTSTRSVLDALGATD
                     GAAMSRVDLAARLLGSSHALDTGTRLERAVTRALSLNGDGDDGRSVWERAGAHSDLVS
                     GAALTWNLPLAGRHPLAPAVTASTGSGVPFVFTQLALRSYPVELMIGSVVLVVENPRV
                     LEHAAQIRSGAAVVCANGNPSATVQLLARQLVAGGVEVRYHGDFDAAGIAMCARMQSV
                     GAMPWRMTAVDYADALAAADAQAVELPVDKHDAPPTPWDPELAVAFNRDRRIVHEERL
                     LGALV"
     gene            complement(2520..3674)
                     /locus_tag="PKY13_08305"
     CDS             complement(2520..3674)
                     /locus_tag="PKY13_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02678 family protein"
                     /protein_id="HOT79975.1"
                     /translation="MSDDQRDDPQAAGEQRDAARHLLMNPITTAERDPAMLRLIRRHE
                     AAVDRWFTQRLGYRLHVDTDTARLYKSTYVPERRPLLATWSTTRPLRQLEYTMLALLC
                     ACTAAGPAVVSLRDLVDEVRSAAAEAGVDLAGDAAERRALVAALRWMIEQGLASELHE
                     QVERYSTDETADAVLRMRPDRIALVPLPTLGDLDFEASAAGSSRGEIPVRQWLRARLV
                     EDPVLYRDDCSDEEWGELRRRLGEESAWLDEMFGVTLESRAEGIAAIDGLGRLSDQRF
                     PTSGTTGHAALLLIERLVAQRVDRSQPVWSQTEVIEIVSDLAERNATVWANDKIEAPP
                     KLAAEVADLLVDLRLARRSDDHDCWLTLLPAAARFFVDPDLVTGVQERLL"
     gene            complement(3671..>5187)
                     /locus_tag="PKY13_08310"
     CDS             complement(3671..>5187)
                     /locus_tag="PKY13_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02677 family protein"
                     /protein_id="HOT79976.1"
                     /translation="RLLTSSPDEARLLFRYVTVEEWRDYRSIMAVFADTFFAEYAPDD
                     VAFALADAGLPIDGDVVADRLEQLRRWGNLDASSSVGSPASLTDYYRRRNRYVISAAG
                     QEVHGLVEGVLTRVDQIRDVSPGRLRAVRDALDALLALNPAVADPSDIADAVRAVFDP
                     HREFTDEVTQFFAAVNQWQTRFDLDPTEFKLFAEVLVGYVGDRLDEIARSARPLGAKL
                     SQLEPALPALLERMSGGLAARVEQAGMSAQVAVSRQVGADRADWDHLAAWFRSEPGQL
                     SRIDQLGRDAVAAIRTLTLNLTRLSRLGVGSSSRRHEFLRLAAAFNGASDVEACHRIA
                     GAALGLFPARHLGVLAADADDPVPTSSSWWTAPRAPVAVSLRQRGTTSNRGAATPLRD
                     RTQELELLRRRRETELADRRHVDAELTAVGHSLDGAELSEAALERLEQLMGRASHLRG
                     RGVHRTGTDAGLTCTMTTQAGHNTVITSPAGRLTLLDTVVVVEPHAEGVAVAPS"
     assembly_gap    5188..5287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5288..9343)
                     /locus_tag="PKY13_08315"
     CDS             complement(5288..9343)
                     /locus_tag="PKY13_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02680 family protein"
                     /protein_id="HOT79977.1"
                     /translation="MAERWKLSRAGIINVYQYGDEVLTFAGGRLLLRGVNGSGKSTAM
                     NMLLPFLLEADVRRIDAAGEQTGVLRSWMLTGREEQQPVGYLWLEFERGGEYLVCGCG
                     IRANQSTPKATTWWFVTPRRPGIDLHLVAGRVPLSTDQLRAEIGQHAVFTHEQRAAYR
                     LKIRADLFGGSDIDQHLRLLHIVRSPRVGDRIDSELTAYLDDALPQLSDAAIDDAAQP
                     LENLEEHRRQVAALAATAETLDGLSEVYRAYAATEIRRRAADALHLVGESRACHRRER
                     AAAATVREAEAECDQARATVAGLESDVERLTSELTALKEQPAYSEGKRLEDLREHVNG
                     LAKLILEARDLVEARLDRLEVARVAVTNAAGVADGDQRLLVGALSEISAIAIDLRLEG
                     GALDAPGVATSTVDDLSDLLVPSGPLAADVLANAASALLGTVAARRGDVAEVRRLLGI
                     ADRAEEALQRAERDLDAARSNALEATERMNRADEALAGTTCAFIDDLRTWASSTAALV
                     EEADQGPAYDAIRGVDDPTAGVDVVDQRVELVNRLAEVVADFAESLRSRRVVIEARRT
                     RETERSAELMAELDRLLTLPEPEVPRAAWQTTTGAAAFGQLIDFADGLAEAERLGYEA
                     ALEASGLLGASVSDGGVTLSIGELVVGGGPPVDSPLSAALRVIVPDDLSGTVDADAVQ
                     RTLDSISTDPASSATTVVAADGSFRLGALQGRHQRSELAHVGSAARFAALQRRRDEVR
                     AELARAEQAVSRSDLELDVITARVDRTTGHGRLLPTTRALDRALDAAERAAEEVERTG
                     ALVEERSNARDARERAVSDTGDAVRRAASTAGLPHSREDLEQADVSLADLAERARGVP
                     HLIRSLSRSCDAWIEAGDRHEVARTEVEVAEDHLARRIAEHQPLAMQLATAQDALGVG
                     YEEIIAAIDQSAAELAEAHAALPGARSIVEHHVGVLADRRAAFATAQGVAAGADRAAS
                     AELPRLRRVLAVTGFREALARALVGDDGEPVVVGSDLPAVAESPDGVAELAGVLLDQV
                     PEPAAPATAESVRQSLRQRRDRLGANWDAGDRQPDESLPCEVEVTGPLGRMPLMTATA
                     AVRRQLAELTSLLTAQQDQALRNLLQGLIAQEVSRKLHAARELVELMNKRLAAVSTAH
                     GISAKLRWTRRDDLDEGLRAIVELMAKLPDLRSEEEDATLAAALSTRLDDARRADPEA
                     PYRQLIGDVLDYRQWHEMAVLLERPGRKPERLSRRTALSEGEKKVVSYLPLFAAVAAS
                     CDALAEAEPSTPRFVLLDDAFAKVSEDNHPKLFGLLVDLDLDFIATSERLWGTYATVP
                     ELAITEVVRDARSGVILLEHARWDGTSRVER"
BASE COUNT         1427 a         3337 c         3183 g         1403 t
ORIGIN      
        1 gcgcccgctc tccactcgat ctcggaggac cggcctttcg gtgcttgcga cccaccctcc
       61 cggggtggtt ttcggccgtt tcgccgcgag atccttgcgt atgaagcgcc acctgccgat
      121 ctgagagagg cgcgacggga tttgttgtgc gacgggaggt gcagaatgac aacagcgagc
      181 ggcctgatcg acgtcagtga cgagtgcgtc ggaacggtcg gactccacgg cctcctcgcc
      241 atggtcgagg aggacaacgg atgctcctga cccggcgttc caagcgaacc gaacccgaga
      301 ccgaacccga agcgccggcg aagtcacccc tcgacgaagc catcgaacgc ctcggccccc
      361 tcaacgccgt cgccgtccag gtgctccgct cgatcgatca tccgatgtcg accggtgcga
      421 ccgtcgccgc cgccatcgag ctcgacccgg tgctcacgag ccaggtgctg cgagtggcga
      481 actcggcgag cttcggtgtg ctcagcaccg tgtcgacgac ggttcgggcc gtcgccgtcg
      541 tcggcatgcc cggcgtccgc tcgatcgcag cggtctccac cgtgcagcgc ggcgctgaga
      601 tgccgcccgg gttctgggag cattccgccg cgacggcagc cggctgcgtc gcagcgtcgt
      661 cggcgatcgg cgtctccaag gacgaggcct tctcgatggg gctcctgcac gatctcggct
      721 gggcgattct cgacgtgctc gatcccgaca cgcaccgctc gatgaccgac gtgctcgacg
      781 acgcgaccct ctgtgcagcc gaggagctcg cgttcggggc cagccacgcc cagatcgcgg
      841 gccggttgct cgagagctgg aacttcccgg ccacgatcgt cgaggcgatc ggcgctcacc
      901 atctggctct cgacgaccat cggtcgaccg tcttgagccc ccacacccag gtcctcctga
      961 tcggtgacgc gctggcgcat cgtctgttgg ccggtgccgg gtaccccttc gccggtgtcg
     1021 atctcggcga gtacgggttc tcggaaccgg cgatcgaggc gttgctcgag ctcaccgacg
     1081 agtacatgcg cgacatcgtg caggtgatgc cccgctgacg cgcggcgagg aaggcgtcgt
     1141 cgaccaactc ctggcgatga ccgccggtgg tgatcctgac gagtcgaccg ccgcaacggt
     1201 gttgctgtcg gacaaggagc acgaggtgct ggcattcctg tccacgctcg cgaactcggc
     1261 ctgatcgccg accgagagcc cggccgggct cgaaaccgac cggcctgatc tgactgtcag
     1321 gggtcccgct cagaccagcg ctccgagcag ccgctcttcg tggacgatcc gacggtcacg
     1381 gttgaaggcc acggcgagtt ccggatccca cggcgtcggc ggcgcgtcgt gtttgtcgac
     1441 cggtagttcg acggcctggg cgtcggcggc ggcgagtgcg tcggcgtagt ccaccgctgt
     1501 catccgccac ggcatcgcac ccaccgactg catccgggca cacatggcga tgcctgcggc
     1561 gtcgaagtcg ccgtgatacc gcacctcgac cccacccgcg acgagttggc gcgccagcag
     1621 ctgcaccgtc gccgacggat tgccgttggc acacacgact gcggccccgg accggatctg
     1681 cgcggcgtgc tcgagaaccc gcgggttctc gacaacgagc accaccgatc cgatcatgag
     1741 ctcgaccgga tacgaccgga gcgccagttg cgtgaagacg aatggcactc ccgaccccgt
     1801 ggaagccgtc accgccgggg cgagcgggtg acgtcccgcg agtggcaggt tccacgtcag
     1861 ggccgccccc gacaccaggt cggaatgggc gccggctcgc tcccagacgc tccgtccgtc
     1921 atcgccatcc ccgttcagcg acagcgcgcg agtcacggcc cgctcgaggc gggtgcccgt
     1981 gtcgagcgcg tgggacgacc ccaacaaccg ggccgcgagg tcaacgcggc tcatggcagc
     2041 cccgtccgtc gcccccagcg cgtccaggac cgagcgggtc gacgtcacca aacgggttgc
     2101 atccggtgcg tcgagtcccg ccaggacgcc cgctcgaatc acaccgtcga tccactcgcc
     2161 ggcccacggt tcgggccacg tcgtcacgat cgagcgtgcc tgctcccgtg ctccggcggc
     2221 ggatgctcgt gccgcccgac gagcagcgac gtcgccgacc gccggatgtc cgagccggtc
     2281 gatggcgtcg gtcagcgact cgccgacgcc gagtcggacg agtccggcct cgaggtcgcc
     2341 cagcgccacc tgcgacttca accgccgacc caccagcgat tcgagcgtca gtcgaccctc
     2401 ggcagagagc gcggggacac ccacgcgccc cttccactcg tcgccgttgt gctcgaggcg
     2461 atcgcgcacc cgcgtccaca gcattgccag ctcgtcagtg gtgagcgact ggggaaccct
     2521 cacaacaagc gctcctgaac cccggtcacc aggtcgggat cgacgaagaa ccgtgccgcc
     2581 gccgggagca gcgtcagcca gcagtcgtgg tcatcggagc gacgagccag gcggaggtcc
     2641 acgaggaggt ccgcaacctc ggcggcgagc ttcggcggtg cctcgatttt gtcgtttgcc
     2701 cacacggtcg cgttgcgctc agccagatcg gagacgatct cgatcacctc ggtctgggac
     2761 cacaccggct gcgaacggtc gacgcgctga gcgaccaacc tctcgatcag caacagcgct
     2821 gcgtgacccg tcgtgcccga cgtggggaac cgttgatccg acagtctacc gagaccgtcg
     2881 atggcagcga tgccctcggc ccgtgactcg agcgtcaccc cgaacatctc gtcgagccac
     2941 gccgactcct cgccgagccg tcgccgcagc tcgccccact cctcatcgct gcagtcgtcc
     3001 cggtagagca ccgggtcctc caccaaccgg gcccgcaacc actggcgaac gggaatctct
     3061 ccccgcgagc ttccagccgc ggacgcctcg aagtcgaggt cgccgagcgt cggcagcgga
     3121 accagtgcaa tgcgatcggg tcgcattcgt aacaccgcgt cggccgtctc gtcggtgctg
     3181 tagcgctcca cctgctcgtg cagctcgctc gccaaccctt gttcgatcat ccatcgcaac
     3241 gcggcgacca gcgcccgccg ctcggcagcg tcgcctgcca ggtcgacccc ggcctcggcc
     3301 gcggccgacc gaacctcgtc gaccagatcg cgcaggctga ccaccgccgg ccccgccgcg
     3361 gtgcaggcgc acaacagcgc cagcatcgtg tactcgagct gccgtagcgg ccgggtggtc
     3421 gaccacgtcg ccaggagcgg ccgccgctcg ggaacgtagg tggacttgta gagacgtgcg
     3481 gtgtcggtgt cgacgtggag tcggtagccg agccgctggg tgaaccaacg atcgacggcg
     3541 gcctcgtggc gacggatcag tcgcaacatc gccggatcgc gctcggcagt ggtgatcggg
     3601 ttcatcagca gatggcgcgc tgcatcgcgc tgctccccag ctgcctgtgg atcgtcgcgc
     3661 tggtcgtcgc tcatgacggc gccactgcca caccctcggc gtgcggctcg acgacgacca
     3721 cggtgtcgag caacgtgagc cgccccgccg gtgaggtgat cacggtgttg tggccggcct
     3781 gggtcgtcat cgtgcaggtg aggccggcgt cggtgccagt ccgatgaacg ccgcgccccc
     3841 ggaggtgcga cgctcgaccc atcagctgtt ccagtcgctc cagcgcggcc tccgacagct
     3901 cggcgccgtc gagtgaatga ccgaccgcag tgagttcggc gtcgacgtgg cggcgatcgg
     3961 cgagctcggt ctcacgtcgg cgcctcagga gttcgagttc ctgggtgcgg tcgcgaagtg
     4021 gggtggcggc gccccggttg gatgtcgtgc ctcgctgccg cagcgacacc gcgacgggtg
     4081 cacgtggtgc ggtccaccac gacgacgagg tcggcaccgg atcgtcggca tcggcagcaa
     4141 gcaccccgag gtggcgcgcc gggaacagac cgagggcggc gccggcgatc cggtggcacg
     4201 cctcgacatc ggacgcgcca ttgaacgccg ctgccagccg gaggaactcg tggcggcgcg
     4261 acgacgagcc gaccccgagc cgcgacaggc gggtgaggtt cagggtgagc gtgcggatcg
     4321 ccgccacggc gtctcggccg agctggtcga tccgtgacag ctgccccggc tccgagcgga
     4381 accaggccgc caagtgatcc cagtcggcgc ggtcggcgcc gacctgtcgt gacaccgcga
     4441 cctgtgccga catgcccgcc tgttcgaccc gcgccgcgag gccacccgac atccgctcga
     4501 gcagcgccgg caacgccggt tcgagctggg acagcttcgc gccgagcggt cgcgccgatc
     4561 gtgcgatctc gtcgaggcgg tcgccgacat accccacgag gacctcggcg aacagcttga
     4621 actctgtggg atcgaggtcg aagcgggtct gccattggtt gacggcagcg aagaactggg
     4681 tgacctcgtc ggtgaactcc cgatgcgggt cgaacacggc gcggaccgcg tcggcgatgt
     4741 cgctcgggtc ggcgacggcc gggttcaacg ccaacagggc gtcgagcgcg tcgcgcacgg
     4801 cgcgcagccg gccgggcgag acatcgcgga tctggtcgac acgcgtcagg acaccctcga
     4861 cgagaccgtg cacctcctgc cccgccgccg agatcacgta ccggttgcgg cggcggtagt
     4921 agtcggtgag gctcgccggc gatccgactg acgacgacgc gtcgagattg ccccagcgcc
     4981 gcagctgctc cagtcgatcg gccacgacgt cgccatcgat cgggaggccg gcatcggcga
     5041 gtgcgaacgc gacgtcgtcg ggcgcgtact cggcgaagaa cgtgtcggcg aacaccgcca
     5101 tgatcgaccg gtagtcgcgc cactcctcga cggtgacgta gcggaacagc aggcgcgcct
     5161 cgtcggggct gctcgtcagc aggcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnntca tcgctcgacc cgggacgtgc cgtcccaccg ggcgtgctcc agcaggatca
     5341 cgcccgagcg ggcgtcgcgg accacctcgg tgatcgccaa ctcgggcaca gtggcgtagg
     5401 tgccccacag gcgctcactg gtcgcgatga agtcgaggtc gagatcgacc agcaacccga
     5461 acaacttcgg gtggttgtcc tcggacacct tggcgaacgc atcgtcgagc agcacgaacc
     5521 tcggcgtcga cggctcggct tcggcgagcg catcgcacga ggcggcgaca gcggcgaaca
     5581 acggcaggta cgacacgacc ttcttctcgc cctccgacag cgccgtgcgg cgactcagcc
     5641 gttcgggctt gcgacccggt cgctcgagca gcacggccat ctcatgccac tgccggtagt
     5701 cgagcacgtc accgatcaac tgccggtagg gcgcctcagg gtcggcccga cgggcgtcgt
     5761 cgaggcgcgt cgacaacgct gccgccagcg tcgcgtcctc ctcttcggag cgcaggtccg
     5821 ggagcttggc catgagttcg acgatcgctc gcaagccctc gtcgaggtcg tcgcggcgcg
     5881 tccatcggag cttggcagag atgccgtgcg ccgtggacac cgccgccaaa cgcttgttca
     5941 tcagctcgac cagctcgcgg gcagcgtgca gcttgcgcga gacctcttga gcgatgagcc
     6001 cctgcaacag attgcggagt gcctggtcct gctgggcggt cagaagactg gtgagctcgg
     6061 cgagctgacg acgcaccgcc gcggtagcgg tcatgagcgg catacgaccc aacggcccgg
     6121 tgacctcgac ctcacacggc aacgattcgt cgggctggcg gtcgccggcg tcccagttgg
     6181 ctccgagccg atcgcggcgt tggcgcaacg actgccggac gctttcggcc gttgccggtg
     6241 ccgccggttc gggaacttga tcgaggagca ctccggccaa ctcggcgaca ccgtcgggag
     6301 actccgctac cgccgggaga tccgacccga cgacaacggg ttcgccatcg tcgccgacca
     6361 acgcgcgcgc taacgcctcg cggaagcccg tgaccgcaag gacacggcgg agccgtggca
     6421 gttccgcact cgccgcccgg tcggcgccgg cagcgacacc ctgggccgtg gcgaacgcgg
     6481 cccggcggtc ggcgagcaca ccaacgtggt gttccacgat cgagcgcgcc cccggaagcg
     6541 ccgcgtgcgc ctcggcgagt tcggcggccg actgatcgat cgccgcgatg atctcctcgt
     6601 atccgactcc gagcgcgtcc tgagccgtcg ccagctgcat cgccaacggc tggtgctcgg
     6661 cgatccgcct ggcgaggtga tcctcggcca cctcgacctc ggtccgggcg acttcgtgtc
     6721 ggtctccggc ctcgatccac gcgtcgcacg atcgcgagag ggagcggatc aagtgcggca
     6781 cgccgcgagc ccgctcggcg aggtcggcca acgacacgtc ggcctgctcc aagtcctcgc
     6841 gggagtgcgg cagccccgct gtggatgcgg cgcgccggac ggcgtcgccg gtgtcggaca
     6901 ctgcccgctc ccgggcgtcg cgtgcgttcg atcgttcctc gaccagcgct ccggtccgct
     6961 ccacctcctc agcggcccgt tccgccgcgt cgagggcacg atcgagggcg cgggtcgttg
     7021 gaagtaaccg accgtgaccc gtggtgcggt cgacccgggc cgtgatgaca tcgagttcga
     7081 gatcggagcg gctgacggct tgctcggctc gggccagctc ggcgcgaacc tcgtcgcgcc
     7141 ggcgctgcag cgctgcgaac cgcgccgccg acccaacatg ggccaactcg gaacgttggt
     7201 gtcgaccttg cagcgccccg aggcggaacg acccgtccgc cgcgacgacc gtcgtggctg
     7261 aggatgccgg gtcggtcgag atcgagtcga gcgtccgttg caccgcgtca gcgtcgacgg
     7321 tccccgagag gtcgtcgggc acgatcactc gcagcgcggc gctcaacggg ctgtcgaccg
     7381 gcggaccacc tccgacgacc agttcgccga tgctgagcgt cactccgccg tcggagaccg
     7441 acgctccgag cagaccagat gcctcgagtg cggcctcgta tccgagccgc tccgcctcgg
     7501 cgagcccatc ggcgaagtcg atgagctgtc cgaacgcggc cgctccggtg gtggtctgcc
     7561 atgcagcgcg cggaacctcc ggctcgggaa gtgtcaacag gcggtcgagc tctgccatga
     7621 gctcggccga acgctccgtc tctctggttc gccgggcctc gatcacgacc cgtcgtgatc
     7681 gcagcgactc ggcgaagtcg gcgacgacct cggcgagtcg gttcacgagc tcgactcgtt
     7741 ggtcgacgac gtcgaccccc gccgtcggat cgtcgacgcc acgaatggcg tcgtaggccg
     7801 ggccctggtc ggcctcctcg accagcgcag cggtgctcga tgcccaggtc cggaggtcgt
     7861 cgatgaaggc gcaggtggtg ccagcgagcg cctcgtccgc tcggttcatc cgctcggtgg
     7921 cctcgagggc attggatcgt gctgcgtcga ggtcgcgttc ggcgcgctgg agcgcctcct
     7981 ccgcacgatc ggcgatgcca aggagacgac ggacttcggc cacatcgcct cgccgcgcag
     8041 cgaccgtccc gaggagggcg ctcgccgcgt tcgccaggac atcggctgcg agcggtccgg
     8101 agggcaccaa gaggtcgctc aggtcatcga cagtggacgt ggcgaccccg ggagcgtcga
     8161 gtgcgccgcc ctcgaggcgg aggtcgatcg cgatcgccga gatctccgac agcgccccga
     8221 cgagcaggcg ctgatcgccg tcagcgacac cggcagcgtt cgtcaccgcg actcgggcga
     8281 cctcgaggcg gtcaaggcgg gcctcgacga ggtcccgggc ctcgaggatc agctttgcca
     8341 gcccgttgac gtgctcacgc aagtcctcca gccgcttgcc ttcgctgtac gccggttgtt
     8401 ccttgagtgc cgtgagttcg gaggtcagtc gttcgacatc gctctcgagg cccgcaaccg
     8461 tcgcccgggc ctgatcgcat tccgcttcgg cctcgcgcac cgtggcagcc gcagcgcgtt
     8521 cgcgccggtg gcaggcccgt gactcgccga ccaagtgcaa cgcgtcggcc gcccggcggc
     8581 ggatctcggt ggcggcatag gcgcggtaga cctcgctcag gccatcgagc gtctcggcgg
     8641 tggccgctag cgccgcaacc tggcgacgat gctcctccag gttctcgagc ggctgggctg
     8701 catcgtcgat cgccgcgtcg gacaactggg ggagcgcgtc gtcgagatac gccgtcagtt
     8761 cgctgtcgat ccgatcgccg acccgcgggc tccgcacgat gtgcagcaat cggaggtgct
     8821 ggtcgatgtc ggaaccaccg aacaagtcgg cgcggatctt gaggcgatac gccgcccgct
     8881 gctcgtgggt gaacaccgca tgctggccaa tctcggcgcg gagctggtcg gtgctcaagg
     8941 ggacccggcc ggcgacgagg tgaaggtcga tcccgggccg tcggggcgtg acgaaccacc
     9001 acgtcgtggc cttcggcgtc gactggttcg cccggattcc acagccgcac acgaggtact
     9061 cgccgccgcg ctcgaactcg agccacaggt agccgactgg ttgctgctcc tcgcggccgg
     9121 tcaacatcca cgaccgcagt acaccggttt gctcgccggc cgcatcgatg cgccggacgt
     9181 cggcctccag gaggaacggc agcagcatgt tcatcgcagt ggacttgccc gagccgttca
     9241 cgccgcgaag caacagacgc cctcccgcga acgtcagcac ctcgtcgccg tactggtaga
     9301 cgttgatgat gcccgctcgg ctgagtttcc agcgctcggc catcggcgga tggtagcccc
     9361 cgaggcatcg gccgacgtcc gccgaccggt cgggccgacc ccaggacgac gctcgtgcca
     9421 gccccggccg agcgtggtcc ccgacaaccc
//
LOCUS       DAOSOA010000104         9362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7300_length_9362_cov_7.044160, whole genome shotgun sequence.
ACCESSION   DAOSOA010000104 DAOSOA010000000
VERSION     DAOSOA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7300_length_9362_cov_7.044160"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PKY13_08320"
     CDS             complement(<1..695)
                     /locus_tag="PKY13_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOT79978.1"
                     /translation="MVGLIAGEAAYQEFVLDSTAKLWLIIAGVIALAAIGLGFVMMKG
                     VLAKDTGTEKMGEIAAAIQEGAMAYIKRQFRTIGIIVVPLAVIVFLTSEAIEKTGNSP
                     DMSGTLSFAQSGTFRTLAFLVGALLSGFIGFLGMWLSTRANLRTAAAAQTGSMNSAMQ
                     VAFKAGGTIGLFTAGLGLLGATLIILIFKNSASAILVGFGFGGSLLALFLRVGGGIFT
                     KAADVGADLVGKV"
     gene            complement(829..1476)
                     /locus_tag="PKY13_08325"
     CDS             complement(829..1476)
                     /locus_tag="PKY13_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOT79979.1"
                     /translation="MLALLPEWLDPETLLSKGGLALLFLIIFAESGLLVGFFLPGDSL
                     LFTAGAVAAGAFAKTLPDLDFNIWVLMIGCFLAAVLGDQTGYLIGKKAGPRLFNREDS
                     KFFKKSHVQKGTEFFEKHGGRAIILARFVPIVRTFVPTIAGVSEMRYRDFLRYNVVGG
                     LIWGVGVTLLGFVFGRIEVVQNNIEVALIGVVVISLLPIVFEVVKHRREAASKTA"
     gene            complement(1537..4545)
                     /locus_tag="PKY13_08330"
     CDS             complement(1537..4545)
                     /locus_tag="PKY13_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOT79980.1"
                     /translation="MRNPEDLPRRRVRWGVSGRGRSVLIAVAVAFVVLLFSLRGLARV
                     YTDFLWFDSLRVAAAWERQWGYQGIMGLLFALAFFALLGTNLLVANRIAPAVVARRTG
                     DELIERYRELVGVRQRAVWLSVSLGFALIAGVGASSQWKNWVLFRYGQSFGQQDPLNK
                     VDLGFYVFKLPFITYLVGWTFAALVIVTLLVAMAHYLTGSLHLPGAGAAAGIKVKAHL
                     SILLASIALVKAIDYYLDRFRMTLSDRGVVTGALYTDVKATLPARLLLVLIAVLVAAM
                     FVANIRRRGWGLPMIGLALWLLMAIVAGTVYPAALQKLKVDSKQSALEAPYIKDNIAA
                     TRGAFGLDRVVERDFDYQDSLSDEEITTNASTVSNVRLLDPDVIPSTFTTLQSKFDFY
                     QFRDLDVDRYKFNGQETEVIIGARELNQQSIPVDTWEGRHLSYTHGYGVAMAPANAVN
                     GDGEPDFAVSDVPVRIDETQLKGLELTRPEIYFGEGLDSEDEAGYAIVGTSLNEESFG
                     GGTSTARQYDGTGGVKVDSFFRKAAFALRFGELEPLTSDYLQDSSRVLYIRGVKERVA
                     EVAPFLQWDHDPYPVLIDGGIKYIVDGYTTSSLVPYSQQADTSYLDADSGLAGLDFNY
                     VRNSVKAVVDAYDGSITMYLTDEMYGGDRDPIIRAYDAAFPELFTPSSEMPDELRSHL
                     RYPEDMFRVQTTMWGRYHLDDPGDFYKQQDRWDVAQNPPEGIRRSGTEAGSAATPIAP
                     NYLQMRLPTESADEFVLFRPFVPHTKDGGDNTKQQLNAFMVGRSDPGQYGNLVLYTMT
                     ETLPDGTVQRNLDVDGPLTAHENMVSDTSTRLSERLTQLNSQGGSSVVKFGNMVLVPI
                     EQGMLYVRPIYVSSERRGSAQQLRIVVVSIGGRVAIGDTLAEALGQLFPTARIATREG
                     GTATPDDPETTPESPGEVTPDDAAELIARAVELFDEADALLRAEGATNLEEYQAKTAE
                     AEELVRKAQESLAVAPAASATTSTSTTTPG"
     gene            complement(4559..7057)
                     /locus_tag="PKY13_08335"
     CDS             complement(4559..7057)
                     /locus_tag="PKY13_08335"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT79981.1"
                     /translation="MSLSGDAPPFDPDDAASREFSKARKGFDPTEVRVHLTKLTEEIK
                     RLRATEADLRAQLEDAATRVPDVESLDPAAISRALGAETVRIIDTAREASDEIRAKAE
                     ENAGRVVRDAHERANRLTRDAAELRETAAREAEELTSSARADADALLAESRAEAEATR
                     SDAAAEAASILEEAALILDERTTEAQLEAVRIRGEAEAARAEAEAYAAQRRADADREA
                     AATAEQAVADAEAKVESARERGREMIREAKEARERMIRDLAERRHNSRQQLEALRAGR
                     ERLIEAFATAREALDEATDELVESLPSARAAADAAARSVDEDLDAAVLELDTLIDRGA
                     DTDELIDGSVDPIDRPLEVPAETGDAQPGEPPVEPVGEVESDELIDELDDDELDEDEP
                     ADGEAAAMIDIALDAADDDDDDVGAADEAADVLVLESVPAGFDVVDMDVEGTVETDDD
                     DDDDDDDFDDDDDDDDFDDDDDEDEDEDGDDAEIPQRGRLRLVSSNEGGPSPVGIEAL
                     VDATAVAEPAEEPRSAEVEELFARLRADRSAVDDDDFDDDFDDDDDDEDDDAPSAEVT
                     TLSTGAVVIDLSAVEAERSVAGGTDVTDVTDDIDLLDDAGAAAQQAMLDQRDALLGDV
                     AQALGRRVRRVVTDQENEVLDLVRRNRKLKGSDDLLPSRDTQIEAYRAAIHKDLREVL
                     AAGADFLAIGNELDDSVVEAALDELLAAVGEWGIDPLRAKLARVVDDSDDTSPDRNEL
                     VDRLRATYREWRNDRIGELSGDIATLGFSRGVMAAASPDQQLCWMVDHGGLPCPDGED
                     NQLAGPVTVGHEFPTGDICPPAHPGCRCLLVPVP"
     gene            complement(7134..8141)
                     /locus_tag="PKY13_08340"
     CDS             complement(7134..8141)
                     /locus_tag="PKY13_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOT79982.1"
                     /translation="MWPWFVAMVAIVVAIAAFVVPLPYYVDGPGVVRATQSRVSVSGH
                     PAYESKGEIFFTTVSERRATPYLLLQAWLDEAIDTLPEKVATPTGDRDAERRAEQQQM
                     DRSKLTSIEVAFRVLDLPLTITGTGAFINEVGKDYPAAAQLRAGDVITAVDGSPVTVV
                     GDLRPLLADKPVGAMVQLAVRRDGTTSDVTVELGRNPDDDSHGYLGVVPSTADEDVDF
                     HFDIELDSGSVIGPSAGLAWTLGVIDRLTPGDLTDGKKVAVTGTIAPDGTVGPIGGIG
                     QKVVGAKEAGATLFLYPAATSPKDVKWLKRLAGDDIRLEPVATVEDALKVLDPTGLGA
                     D"
     gene            8178..8537
                     /locus_tag="PKY13_08345"
     CDS             8178..8537
                     /locus_tag="PKY13_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79983.1"
                     /translation="MDPTLLIGIAVVALGTGVGALMMFGQRANSLAEASGSPGTIPVA
                     PATSPSELSGSSAQYETPEELRPPVPRGLRSTDVHIRWYQRLRAGLGLLLITVGIGMA
                     FGAVLGLGALALSLFLG"
     gene            complement(8680..9138)
                     /locus_tag="PKY13_08350"
     CDS             complement(8680..9138)
                     /locus_tag="PKY13_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79984.1"
                     /translation="MSHMVIFQTPDGNPGYNQFETVDESIGFVEKLRNEQNVLNARIF
                     KLEELKFEFKPYFRVQLAMLNSGDAPAAAPVAAAPAPVAPVAAPAPAPAPAAPFGGAP
                     AAEAPAAPPAPQGAPVSNIDSPVGAPVPNGNSFSGAPAEDDRGRRGLFGR"
BASE COUNT         1490 a         3195 c         3156 g         1521 t
ORIGIN      
        1 tcgaccttgc cgaccaggtc ggcgccgacg tcggcagcct tggtgaagat gccgccgccg
       61 actcgaagga agagtgcgag cagcgaaccg ccgaagccga acccgaccag gatggccgac
      121 gccgagttct tgaagatcag gatgatcagc gtggcaccca gaagcccgag gccggccgtg
      181 aacagaccga tcgtgccgcc ggccttgaac gccacctgca tcgccgagtt catcgaaccg
      241 gtctgcgccg cagccgcggt gcgcaggttg gcccgcgtcg acaaccacat gccgaggaac
      301 ccgatgaagc ccgagagcag ggcgccaacg aggaaggcga gggtacggaa cgtgcccgac
      361 tgggcgaacg acagcgtgcc gctcatgtcg ggagagttgc ccgtcttctc gatcgcctcc
      421 gacgtcagga acacgatgac cgccaacgga acgacgatga tgccgatggt gcggaactgg
      481 cgcttgatgt acgccatcgc gccttcctgg atcgccgcag cgatctcgcc catcttctcg
      541 gtgccggtgt ccttggccag cacccccttc atcatcacga agccgagtcc gatcgcagcg
      601 agcgcgatga cgcccgcgat gatgagccac aacttggccg tcgaatccag gacgaactcc
      661 tggtacgccg cttctccggc gatcagtcca accatgggtg gtcctctctg ttcgaaaggg
      721 cgcgccaaga cgacgtgccc cacctcgttc accccgtgtc gtccatgctc accgcgagcg
      781 gtgtgaccga cacacggggg gagagtccag ccgagttggc tgggcgcgtc aagcggtctt
      841 ggaggccgct tcgcgccggt gcttcacgac ctcgaacacg atcgggagca gcgagatcac
      901 gaccaccccg atgagcgcga cctcgatgtt gttctgcacg acctcgatcc gcccgaacac
      961 gaagccgagc agcgtcacac cgactcccca gatgaggccg ccgacgacgt tgtaccgcag
     1021 gaagtcgcgg tacctcatct ccgagacacc ggcgatcgtc ggcacgaagg tgcggacgat
     1081 cggcacgaag cgggcgagga tgatggcccg gccgccgtgc ttctcgaaga actcggtgcc
     1141 cttctggacg tgactcttct tgaagaactt cgagtcctct cgattgaaca accggggccc
     1201 ggccttcttg ccgatcaggt agccggtctg atcgccgagc accgctgcga ggaaacatcc
     1261 gatcatcagg acccagatgt tgaagtcgag atcggggagc gtcttggcga acgcgcccgc
     1321 tgcgaccgct ccggcggtga agagaagcga gtcacccggc aggaagaatc cgaccaacaa
     1381 tcccgactcg gcgaagatga tgaggaacag cagggcgaga ccgcccttgc tgagcagggt
     1441 ttcgggatcg agccattccg gcagcagcgc aagcacgagg tgggagcgta gccgtggacc
     1501 cccacactcc ccgagtcagg ggcaggggca gggcgttcag cccggcgtcg tcgtcgatgt
     1561 cgacgtggtc gccgacgctg cgggagcgac ggcgagcgac tcctgcgcct tgcgcaccag
     1621 ttcctccgcc tcggcggtct tcgcctggta ctcctcgagg ttcgtcgcgc cctcggctcg
     1681 caacagggcg tcggcctcgt cgaacagctc gacggcgcgg gcgatgagct cggcggcgtc
     1741 gtcgggggtg acctcgcctg gcgactccgg cgttgtctcg gggtcgtcgg gggtggccgt
     1801 gcccccctcg cgcgtcgcaa tgcgcgccgt ggggaacagc tggccgagcg cctcggccag
     1861 cgtgtcgccg atcgccaccc gaccgccgat cgacaccacg acgatgcgca gctgctgcgc
     1921 cgaccctcgc cgttcgctcg aaacgtagat cgggcgcacg tacaacatgc cctgctcgat
     1981 ggggaccagc accatgttgc cgaacttcac gaccgagctg ccgccctgcg agttcagctg
     2041 ggtgagccgc tcggacagac gggtgctcgt gtcggacacc atgttctcgt gcgcggtcag
     2101 cggcccgtcg acatcgaggt ttcgttgcac ggtgccgtcg ggcagcgtct cggtcatcgt
     2161 gtagaggacc aggttgccgt actggcccgg atcgctccga ccaaccatga aggcgttgag
     2221 ctgctgcttg gtgttgtcgc cgccgtcctt cgtgtgcggc acgaacggcc ggaagagcac
     2281 gaactcgtcg gcgctctcgg tcgggaggcg catctgcagg tagttgggag cgatcggggt
     2341 cgccgccgac cccgcctcgg tgcccgagcg acggatgccc tcgggcgggt tctgcgcgac
     2401 gtcccaccgg tcctgctgct tgtagaagtc acccgggtcg tcgaggtggt accgacccca
     2461 catcgtcgtc tgcacccgga acatgtcctc ggggtagcgg aggtgggagc gcagctcgtc
     2521 gggcatctcc gagctcgggg tgaagagctc ggggaacgcg gcgtcgtagg cccggatgat
     2581 cggatcccgg tcgccgccgt acatctcgtc ggtcaggtac atcgtgatcg agccgtcgta
     2641 ggcgtcgacc acggccttga ccgagttgcg cacgtagttg aagtcgaggc cggcaaggcc
     2701 cgagtcggcg tcgaggtagc tcgtgtcggc ctgctgggag tacggcacga gcgacgaggt
     2761 cgtgtagccg tcgacgatgt acttgatgcc gccgtcgatg aggacggggt acggatcatg
     2821 gtcccactgc aggaacggcg cgacctcggc gacccgctcc ttcaccccgc ggatgtagag
     2881 cacacgcgac gagtcctgca ggtagtccga cgtcaacggc tcgagctctc cgaagcgcaa
     2941 cgcgaacgcc gcctttcgga agaacgagtc gaccttcacc ccgcccgtgc cgtcgtactg
     3001 acgagcggta ctcgtaccgc cgccgaaact ctcctcgttg agcgaggtcc cgacgatcgc
     3061 gtatcccgcc tcgtcctcgc tgtcgagacc ctcgccgaag tagatctcgg gccgcgtgag
     3121 ttcgaggccc ttgagctgcg tctcgtcgat gcgcaccggc acgtcgctga ctgcgaagtc
     3181 gggttcaccg tcgccgttga ccgcgttggc gggggccatc gcgacgccgt agccgtgggt
     3241 gtaggagagg tgtcgccctt cccaggtgtc gaccgggatc gactgctggt tcagctcgcg
     3301 ggccccgatg atgacctcgg tctcctggcc gttgaacttg tagcggtcca cgtccagatc
     3361 gcggaactgg tagaagtcga acttcgactg cagggtcgtg aacgtcgacg ggatgacatc
     3421 gggatcgagc aagcggacgt tcgacaccgt cgacgcgttg gtggtgatct cctcgtcgct
     3481 cagggagtcc tggtagtcga aatcccgttc gacgacgcgg tcgagtccga acgccccccg
     3541 ggtcgcggcg atgttgtcct tgatgtacgg cgcctcgagg gccgactgct tcgagtccac
     3601 cttcagcttc tgcaatgccg ccgggtacac agtgcccgca acgatcgcca tcagcagcca
     3661 cagcgcgagg ccgatcatcg ggaggcccca tccccgccgc cggatgttgg cgacgaacat
     3721 cgcagcgacc aacaccgcaa tgaggacgag cagcaacctc gccggcaggg tcgccttcac
     3781 gtcggtgtag agggcacccg tgacgacccc acgatcggac aacgtcatgc ggaaccggtc
     3841 gaggtagtag tcgatcgcct tcaccagcgc gatcgaggcc aggaggatcg agagatgcgc
     3901 cttgaccttg ataccggcgg cagcgccggc tccggggagg tgcagcgagc cggtgaggta
     3961 gtgcgccatg gcgacgagca acgtgacgat gacgagcgca gcgaacgtcc agccgacgag
     4021 gtaggtgatg aagggcaact tgaagacgta gaagccgagg tcgaccttgt tgagcggatc
     4081 ctgctggccg aagctctgcc cgtaccggaa gagcacccag ttcttccact gcgacgacgc
     4141 cccgacgccg gcgatcagcg cgaagccgag cgacaccgac aaccagacgg cacgctgccg
     4201 aactccgacg agctcgcggt accgctcgat cagttcgtca ccggtacggc gggcgacaac
     4261 ggcgggcgcg atgcggttgg ccaccaacag attcgtgccg aggagggcga agaacgccag
     4321 cgcaaacagc agtcccatga tgccctggta gccccactgg cgctcccagg ccgcggccac
     4381 gcgaagcgaa tcgaaccaca ggaagtcggt gtagacccga gccagcccgc ggagcgagaa
     4441 gaggagcacc acgaacgcca ccgccacggc gatgagcacg gagcggcctc gccccgagac
     4501 tccccagcgg acgcggcgac ggggcaggtc ctctgggtta cgcatcaggc cgagcgtact
     4561 aagggacggg caccaacaga caccggcacc cggggtgcgc cggtgggcag atgtcacccg
     4621 tggggaactc gtggccgacc gtcaccgggc cggcgagctg gttgtcctcc ccgtcgggac
     4681 acggcagccc cccgtggtcg accatccagc acagctgttg gtcgggggac gctgccgcca
     4741 tcacgccgcg gctgaacccc agcgtggcga tgtcgccgga cagttcgccg atccgatcgt
     4801 tgcgccactc ccggtaggtg gcgcgcaatc ggtcgaccaa ctcgttgcgg tcgggtgacg
     4861 tgtcatcgct gtcgtcgacc acccgggcca acttggctcg gagcggatcg atcccccact
     4921 ccccgacggc ggccagcaac tcgtcgagcg ctgcctcgac cacggagtcg tcgagctcgt
     4981 tgccgatggc aaggaagtcg gcaccagcgg cgagcacttc ccggaggtcc ttgtggatcg
     5041 cggctcggta ggcctcgatc tgggtgtcac ggctcgggag cagatcgtcg gagcccttga
     5101 gcttgcggtt ccggcgaacg aggtcgagga cctcgttctc ctggtcggtc accacccgcc
     5161 ggacgcgtcg gccgagggcc tgggcaacgt cgccgaggag ggcgtcgcgc tggtccaaca
     5221 tcgcctgttg cgccgctgcc ccagcgtcgt cgagtaggtc gatgtcgtcg gtcacgtcgg
     5281 tcacgtcggt gccgccggca accgaccgct cggcctcgac cgcactgagg tcgatgacca
     5341 cggcgccggt cgacagggtc gtcacctcgg ccgagggtgc gtcgtcgtcc tcgtcgtcgt
     5401 cgtcgtcgtc gaagtcgtcg tcgaagtcgt cgtcgtcgac cgccgaccgg tcggctcgca
     5461 ggcgagcgaa caactcctcg acctcggcgg accggggctc ctcggccggc tcggccacgg
     5521 cggtggcgtc gacgagcgcc tcgatgccga ccggcgacgg cccgccctcg ttggaactca
     5581 ccagtcggag ccggccccgc tggggaatct cggcgtcgtc gccgtcctcg tcctcgtcct
     5641 cgtcatcgtc atcgtcgaag tcgtcgtcat cgtcgtcgtc atcgaagtcg tcgtcgtcat
     5701 cgtcatcgtc gtcgtcggtc tcgaccgtgc cctcgacgtc catgtccacc acgtcgaacc
     5761 cggccggcac ggactcgagg acgaggacgt ctgccgcctc gtcggcggca cccacatcgt
     5821 catcgtcgtc atcagcagca tcgagcgcga tgtcgatcat cgccgctgcc tcgccgtcag
     5881 ccggctcgtc ctcgtccagc tcgtcgtcgt ccagctcgtc gatcagttcg tcggactcca
     5941 cctcgccgac cggttcgacc ggcggttcgc cgggctgagc atcgccggtc tccgccggga
     6001 cctcgagcgg tcggtcgatc ggatcgacag agccgtcgat cagttcgtcg gtgtcggcgc
     6061 cgcggtcgat cagcgtgtcg agctcgagca ccgctgcgtc cagatcctcg tccacggacc
     6121 gggccgcggc atcggcggcc gcccgtgccg acggcaacga ctcgaccagc tcgtcggtgg
     6181 cctcgtcgag ggcctcgcgg gccgtcgcga acgcttcgat gagccgctcc cgaccagccc
     6241 gaagcgcctc gagctgctgg cgggagttgt gtcggcgctc ggccaggtcg cggatcatcc
     6301 gctcccgggc ctccttggcc tctcgaatca tctcccggcc gcgctcgcga gcggattcga
     6361 ccttggcctc ggcgtcggcg accgcctgct cggccgtcgc cgctgcctcg cggtcggcgt
     6421 ccgcccgtcg ttgggcggca tacgcctcgg cctcggcgcg agctgcctcc gcttcgccac
     6481 gaatgcgaac cgcctcgagc tgggcctcgg tggtccgctc gtcgaggatc agcgcagctt
     6541 cctcgagaat ggaggctgcc tccgccgccg cgtcggaacg tgtcgcctcg gcctcggctc
     6601 gcgactccgc gagcagggcg tcagcgtcgg cccgagcgct cgacgtcagc tcctcggcct
     6661 ctcgtgccgc ggtctcccgc aactcggctg cgtcccgggt gaggcggttg gcccgttcgt
     6721 gagcatcccg aaccacacga ccggcgttct cctcggcctt ggcgcggatc tcgtcgctcg
     6781 cctcgcgagc ggtgtcgatg atgcgcaccg tctccgcgcc gagggcccga ctgatcgctg
     6841 cgggatccag cgactccaca tcggggaccc tggtggcagc gtcctcgagc tgggcgcgga
     6901 ggtcggcctc ggtcgcccgc aaccgcttga tctcctcggt cagcttggtg aggtggacgc
     6961 gcacttcggt ggggtcgaac cccttgcggg ccttggagaa ctcccgactc gctgcgtcgt
     7021 ccgggtcgaa cgggggcgcg tcgcctgaga gactcatgac tcgggatgct acgaccgatc
     7081 cggcgcctcg gggggtatac cccccgacgc ccccgcactg ctggaccgaa ccctcaatcg
     7141 gccccgagac ccgttggatc gagcaccttc aacgcgtcct cgacggtcgc caccggctcc
     7201 aggcggatgt cgtcgccggc caggcgtttc aaccacttca cgtccttcgg actggtggct
     7261 gcggggtaga ggaagagcgt cgcgccggcc tccttggccc ccaccacctt ctggccgatc
     7321 cccccaatgg gaccgaccgt accgtcgggc gcgatggtgc cggtcacggc gaccttcttg
     7381 ccgtcggtca ggtcacccgg ggtgagccga tcgatcaccc cgagcgtcca ggcgagaccg
     7441 gcggacggtc cgatgaccga gccggaatcg agctcgatgt cgaagtggaa gtcgacgtcc
     7501 tcgtcggcgg tcgacggcac gacaccgagg tagccgtggc tgtcgtcatc gggattgcgt
     7561 ccgagctcca cggtcacgtc ggacgtcgtc ccgtcgcggc gcaccgccag ctggaccatc
     7621 gcaccgaccg gcttgtcggc aagcaggggg cgcaggtcgc cgacgacggt gacgggcgat
     7681 ccgtcgaccg ccgtgatgac gtcgccggcg cgcaactggg cggccgcggg atagtccttg
     7741 ccgacctcgt tgatgaaggc gcccgtgccg gtgatggtga ggggcaggtc gagtacccgg
     7801 aacgccactt cgatcgacgt cagcttcgaa cggtccatct gctgttgttc cgcgcgtcgc
     7861 tcggcgtccc ggtccccggt cggggtcgca accttctccg ggagcgtgtc gatcgcctcg
     7921 tcgagccatg cctgcaacag gaggtagggg gtcgcccgtc gctccgacac tgtcgtgaag
     7981 aagatctcac ccttcgactc gtaggcggga tgacccgaga ccgacacgcg ggactgcgtg
     8041 gctcggacca caccaggccc gtcgacgtag tagggcagcg gaacgacgaa ggccgcgatg
     8101 gcgaccacga tggccaccat cgccacgaac cacggccaca cccgcccacg tgaccgtgcc
     8161 gatagcatgt cggggtcatg gaccccacgc tgctcatcgg catcgcggtt gtcgctctcg
     8221 gaaccggagt cggagccttg atgatgttcg gacaacgcgc caactctctc gccgaggcct
     8281 cagggtctcc gggcacgata cccgtcgctc ccgccacttc cccgtccgag ctgtcggggt
     8341 cctcagctca gtacgagact cccgaagaac tgcgaccccc ggtcccccgt ggtctccgct
     8401 cgactgacgt ccacatccgt tggtaccaac ggcttcgagc cgggctcgga ctgctcctga
     8461 tcaccgtcgg catcgggatg gcgttcggcg cggtcctggg cctcggcgca ctggccctca
     8521 gcctcttcct gggctgagcc cggacgcgcc acagcgcacc ggcggaccgg tgcgctgtgg
     8581 ttcgaatctg ttccggcggg cgagcgcgac cgacctgctg cgtgtcgcgt ggttcgcatc
     8641 ggccgacccg ttctggtgac ggtcgaccgg tcggtgggct cagcgcccga acagaccccg
     8701 gcgaccgcgg tcgtcctcgg cgggagcgcc ggagaacgag ttgccattgg gcaccggagc
     8761 gccgaccggc gagtcgatgt tcgacacggg cgcaccctgg ggagccggcg gagcggcagg
     8821 cgcctcggca gccggagcgc caccgaacgg cgccgcggga gcgggggccg gggccggggc
     8881 ggcgaccggg gcgaccggag ccggagcagc ggcaaccggg gcagcggcag gggcgtcgcc
     8941 ggagttcaac atcgccagct ggacacggaa gtagggcttg aactcgaact tcagctcttc
     9001 gagcttgaag atgcgggcgt tcaggacgtt ctgctcattc ctgagcttct cgacgaagcc
     9061 gatcgactcg tcaaccgtct cgaactggtt atagcccggg tttccatctg gggtttgaaa
     9121 gatgaccatg tgtgacacga gcgcgtctcc ttaagcaggc acggggaagg ctcaggagtt
     9181 taggctcgcg acatggccgc gcgtccacag aatctttcca gaagatccgt ggtcatcgcg
     9241 ggatggtcga atgatgagtc gggggcccgg cagcagctgg gctccgacga cggggctgtg
     9301 cgtgcttcag cggtgtcagc gctggcccga atgggcgctc tcagtatccc agacctcctc
     9361 gc
//
LOCUS       DAOSOA010000105         9287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7366_length_9287_cov_6.093263, whole genome shotgun sequence.
ACCESSION   DAOSOA010000105 DAOSOA010000000
VERSION     DAOSOA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7366_length_9287_cov_6.093263"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2348
                     /locus_tag="PKY13_08355"
     CDS             <1..2348
                     /locus_tag="PKY13_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79985.1"
                     /translation="PGSDLDGSFGSNGVARVDFPKQAQMVESVATTSGGTTFLLTENG
                     GANYSRTLRRISASGQVDPSFGTNGSIVLSRCSGCEIAVDELNHLYVFSPGLDPIGVP
                     AIYVTRYSSDGVVDTSYGTAGRAYFNTTTFQMVSVGRQGSVAIVDAINPRVAMISADG
                     KNVNVVATNHCSVVYGGAFDSVGRFLFLSGRCTPIAPSGSTYVQSVFRLTASGLDSAF
                     DTDGVANVPGTNGLANRLLVDSANRPLVFSDRSVLRMTTAGSPDGSYHNGTAATLPET
                     GGRTQATAVAINTNDEVFGIVHTPSGQYNEDFAISKLLASGQPDPQFGIGGVVVPSVF
                     AGPLTSTFGMAPFNSPILMTSGSGVLISSSVQATDPSFGPLIANGFARLDGQGAAVSG
                     YGSNSVLALRDAPLMLPVGATWPGPNGSVYVSFERDRGVGVVLARFAASGGLDTSFSG
                     DGLIVDPLATSVTVDPTGRPAFFRVTVSAGTLSFGMQTGPTARSAARFTTTGSPDPTF
                     GGGTVTWPELTLPAGATSYCDALLTATSDGAIVVAYGPAGGVAECSTTGLDRISATGV
                     LTQGWAPAMSSLAGTRVVKTTAAASNGRTVVLLATPGAQDSRMVRLTPAGDIDSGFGS
                     AGVINIGDSGSSQLGVLNDDSVVVSATSGSANSFSAGGIDIRRFLVDGTPVTTGGFGA
                     TGRMHSTGTQRTTVAGSHIVTAGFNADAGVDSTEGFVLAARSDGAAETSFPRLGDDGR
                     ADFASNFSVLHYPVVSGQRAFVASLSITGVVEIRKFSGTLS"
     gene            2432..3679
                     /locus_tag="PKY13_08360"
     CDS             2432..3679
                     /locus_tag="PKY13_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79986.1"
                     /translation="MILLAALVAVVAGCTDADSSSSPSNTGATVSTTVIRATTSVPPP
                     PAATMPGAFAASGPISAPVRIGPPAGSALDGIDRDAGVSVRLRDGSTLWLFGDTARAD
                     DNGALEYFEIGTAAWAAPGEPTITRDVDQGGTPVAFATPQAGFPPCPGSAPTPGMWPS
                     SAVVQPLGDVDRVIVWMENICLGSSIHGAARGIAVAEWIYDPARPPTDAPIRARILNQ
                     TLFPQRSFGLASLVDDDGRAIVYTCQAPEQGGMPEQYGPCHAAKVDLDRVADPTAYST
                     WTGQDFTGNVMSSMPLSFEPGSIAAPLPAGSFSVTKDPHLGRYVMVYSPWPGYSDSLV
                     IRTATAPTGPWSVPTVVPLPNCSDAVGPITFWCYAASAQPIFSEPGKLGVGYYDRSVS
                     TGPVRGSYLVATVPYDAAVPAGG"
     gene            complement(3649..5004)
                     /locus_tag="PKY13_08365"
     CDS             complement(3649..5004)
                     /locus_tag="PKY13_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOT79987.1"
                     /translation="MDIAYLLIGLLLGSAGGIWLAVQARSALADAARESARREEFHVD
                     RANRQAEREALLREHIARVEAAAATDDQMLDAFRSLSQEALAAQSAQLVQLAEAKYGA
                     LHQTTDSVLAGHSKAVGDGLRALSERLVALERERNDATSALRAIVGDLSVAHRATQAE
                     TSKLAAAMRDNRVRGAWGEVQLRRVLEMAGLDRHADFIEQRGVTDGESRSRPDVVVPL
                     PNGRCVVIDAKVPLDRYLDAANAGDPAIERRLQAEHAKAVAGHVTALAQRDYAAKVDG
                     SVDMVLLFLPGEPFLAAALDADPTLFEAAAAKGVHLVTPSSLVPLLRGIALGWREHQA
                     ERAAAEIQQLGIELHERIAIFAEHFAAVGSQLDRTVNAFNRSVGSLDLRVVSTARKLS
                     EHGAGSTRTVPDVVEVPTVSRPIRMLELIVASRPDESTAPLSAAAGESPGQPPAGTAA
                     S"
     gene            complement(5098..5898)
                     /locus_tag="PKY13_08370"
     CDS             complement(5098..5898)
                     /locus_tag="PKY13_08370"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HOT79988.1"
                     /translation="MRILGIDLAAQPRNTGVVVLDPVEPGRWRADLPPEVATDEHLVE
                     LGGHVDLIGVDAPLGWPIAFVQAVRAHEDREPWPGTKDRRSLTHRHTDNVVRDHGRGQ
                     PMSASADLLGHVAMRCALLQRDWAERWGGAAPRDGSGRLVEVYPAAALRAWHLPERGY
                     KGQSAEAGRVRKAILAGIVDDCSTWLDLDRVRSACIDSDHTLDALLSALNALAARAHQ
                     TFPPESVEDHHCAVLEGWIHVPSVPLASVRPPVDPASLVDPARDESPF"
     gene            5988..>7324
                     /locus_tag="PKY13_08375"
     CDS             5988..>7324
                     /locus_tag="PKY13_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT79989.1"
                     /translation="MSSTIIRGGTVFDGTGSDGRRADVLIENGLVARIDHNIEVGPDV
                     EVVDANGCWVTPGFIDLHTHYDAELEIDPSLVESVRHGITTVLVGSCGLSFAVGEPED
                     LADMFCRVEGVPRAEVLPLLEQVQDWDSPKGYLDHLERLPLGPNVISFLGHSAIRAHV
                     MGLGRALDDTVTPTRSELDRMASMLDEALDVGYLGLSINTLPWDKMDGDRYRSRPTPS
                     VFANWKEYRFLGEVLRRRDAVLQGVPDLSGRWNFALFVAIASGIRRKPLRVSIISMLD
                     AKAAPGAFKVIGGAMTFARRYLRADVRLQSLPQPFRLWTDGLENPVIEEIGAGTEALH
                     LATNARGDLLRDPVYRERFRKQWSNKLKGRAYHRDLAEARIVECPDDTLVGLTFAEVA
                     VRRGRDRIDTFLDLQAEFGNDLRWTTTVGNTNEESVAWIVNHPQAQIGFSDAGA"
     assembly_gap    7325..7424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7425..7698)
                     /locus_tag="PKY13_08380"
     CDS             complement(<7425..7698)
                     /locus_tag="PKY13_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79990.1"
                     /translation="MPSHSMPSGVVVAGPDHWSDPIAHTPGRPDHQMVDVVTGAADRQ
                     PVEEVRVRRERGERRRRGALGFGTSTPSGAGPVIAHGLAPSRRDGRR"
     gene            7682..8518
                     /locus_tag="PKY13_08385"
     CDS             7682..8518
                     /locus_tag="PKY13_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79991.1"
                     /translation="MLWLGTTPVSVEADHMSSRAEYARHALAGEVRPVLCVVGAQLPQ
                     PVHRINGVDVVDADWLVSLLTSHPTILSTVEVDAAIHAATRWRVQPPDHVPERWVPPI
                     FESSRALPPPPPQRFDRDPRRVSASQVMIAATVVAVSMLGLIGYLLTADRGTVSAFPQ
                     TASMASGRVVVTDVPGYGALRVEVVCPAEGGRFDLVGHVQGVYDGRVRIATTVGGEGR
                     YLGEFSSLQRTPVISVLATATDTTFSVQRVDRAGRGGEVSTFAVTTPAEPCRPASEPT
                     GG"
     gene            8589..>9287
                     /locus_tag="PKY13_08390"
     CDS             8589..>9287
                     /locus_tag="PKY13_08390"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HOT79992.1"
                     /translation="MRRIDLSAAPHDVRTVPARSFAGRTGRWWLAVTSLAIAAIAPLP
                     YLTSGLGDMAREGQEIAGNYVDRPGWLQVAFYVHVCLGGLAMLTAPVQLSSRVRRRAP
                     RLHRVAGRVGIASILVAAIAGAVLSPYSVADGVGFAGFGLLAVAWFTCALASYRTIRR
                     GDVAAHRRWAIRTFALTYAAVTLRLWLGVMIGVQAGIMGVDGDLAFDRAYHIVPFLAW
                     VPNLILAEVIIDRTT"
BASE COUNT         1307 a         3266 c         3188 g         1426 t
ORIGIN      
        1 cgcccggctc cgatctcgac ggatcgttcg ggtcgaacgg tgtcgcgagg gtggacttcc
       61 cgaagcaggc ccagatggtc gagtcggtgg ccaccacgtc gggtggaacg acgttcctgt
      121 tgaccgagaa cggcggcgcc aactactcgc gcacactccg acgcatctcg gcctccggcc
      181 aggtcgaccc gtcgttcggc accaacggca gcatcgtcct ctcccgttgc tccggctgcg
      241 agatcgctgt cgacgagctg aaccatctgt acgtgttctc gcccgggctc gacccgatcg
      301 gtgtcccggc gatctacgtc acccgctaca gctcggacgg ggtcgtcgac acgtcgtacg
      361 gcacggccgg ccgtgcctac ttcaacacga ccacgttcca gatggtttcg gtcggccggc
      421 agggtagcgt cgccatcgtc gacgcgatca accctcgggt cgccatgatc tcggcggacg
      481 ggaagaacgt gaacgtggtc gccacgaacc actgcagcgt cgtgtatggc ggagccttcg
      541 actccgtcgg gagattcctc ttcctgtccg gtcgttgcac cccgatcgct ccctcgggat
      601 cgacgtacgt ccagagcgtg ttccgcctga cggcaagtgg cctcgattcg gcgttcgaca
      661 ccgacggtgt cgcgaacgtt cccggcacga atggtttggc gaatcgactg ctggtcgact
      721 ccgccaaccg tcccctcgtg ttctccgacc gttccgtgct gcggatgacg acagcgggtt
      781 cgcctgacgg gtcctaccac aacgggacgg cggcgacgct gcccgagacc gggggccgga
      841 cccaggccac ggcggtggcc atcaacacca acgacgaggt gttcggaatc gtccacaccc
      901 ccagcggtca gtacaacgag gacttcgcca tctccaagtt gctggcgtcg ggccaacccg
      961 atccgcagtt cggcatcggc ggggtcgtcg tgccgtcggt gttcgccggt ccgctcacgt
     1021 cgacgttcgg aatggccccg ttcaactcac cgatcctgat gacctcgggg agcggcgtgc
     1081 tgatctcgtc gagcgtgcag gccactgatc cctcgttcgg accgctgatc gccaacggct
     1141 tcgcgcgcct cgacggacag ggcgcggcgg tgtccggata cggttcgaac tcagtgctcg
     1201 cactccgcga cgctccgctg atgctccccg tcggcgcgac ctggccgggt ccgaacgggt
     1261 cggtctacgt ctccttcgaa cgcgaccgtg gggtcggcgt cgtgctcgcc cgcttcgctg
     1321 cgtccggcgg cctcgacaca tcgttcagtg gtgacggcct catcgtcgat ccgctggcga
     1381 cgtcggtgac cgtcgatccg accggacggc cggcgttctt cagggtcacc gtctcagccg
     1441 gcaccctgtc gttcggcatg cagaccggcc cgaccgctcg ttcggcggct cgattcacga
     1501 cgacggggtc accggacccg acgttcggcg gcgggacggt gacctggccc gaactgacgc
     1561 ttccggcggg agcaacgtcc tactgcgacg cgctcctgac tgcgaccagc gacggagcga
     1621 tcgtcgtggc gtacgggcct gccggcgggg ttgccgagtg ttcgacaacg ggcctcgacc
     1681 gaatctcggc gaccggggtc ctcacccagg ggtgggcgcc ggccatgagc tcgctcgcgg
     1741 gtacccgggt ggtgaagacc acggctgcag cctccaacgg gcggaccgtg gtgctcctcg
     1801 cgacaccggg cgcgcaggac tcacggatgg tccgactgac gccggccggc gacatcgact
     1861 ccgggttcgg gagtgccggt gtcatcaaca tcggcgacag cggctcttcc cagctcggcg
     1921 tgctgaacga cgactcggtg gtggtctccg cgaccagcgg atcggccaat tcgttcagcg
     1981 ccggaggcat cgacatccga cggttcctcg tcgacggcac gccggtcacg accggcggct
     2041 tcggcgccac ggggcgaatg cactcgaccg ggacgcagcg cacgaccgtc gctggctcgc
     2101 acatcgtgac cgccggattc aacgccgacg ccggggtcga ctcgacggaa ggctttgtcc
     2161 tcgcggcacg ctccgacggc gctgccgaaa cgtcgttccc cagactgggc gacgacggtc
     2221 gggccgactt cgcctcgaac ttctcggtgc tccactaccc cgtcgtgtcg ggtcaacggg
     2281 cgttcgtggc atcgctgagc atcaccgggg tcgtggagat ccggaagttc tccggcacac
     2341 tctcctgacg tttccgatca cagttcccct ctcgttccga tcccgttcga cgctccgcgc
     2401 cggtcaccgg ctcgcgcccc gaacattggg cgtgatcctg ctcgcagcgc tcgtcgccgt
     2461 cgtcgcaggt tgcaccgacg cggactcgtc gtcgtccccc agcaacaccg gggcgacggt
     2521 gtccaccact gtcatccgtg caaccacctc ggtcccgccg ccaccggcgg caacgatgcc
     2581 gggcgcgttt gccgcctccg ggcccatctc ggcgcccgtc cggatcgggc cgccggccgg
     2641 gagcgcgctc gacggcatcg atcgcgacgc cggtgtgagt gtgcggctcc gcgacggctc
     2701 gaccctctgg ctgttcggcg acacggcgag ggccgacgac aacggtgcgc tcgagtactt
     2761 cgagatcggc acggcggcct gggcggcgcc gggcgaaccg accatcacga gggacgtcga
     2821 tcagggaggc accccggttg cgttcgcgac gccccaggcc ggcttcccgc cgtgcccggg
     2881 ttcggccccg actcccggca tgtggccgtc gtccgccgtc gtccaaccgc ttggcgatgt
     2941 cgaccgcgtc atcgtctgga tggagaacat ctgcctcggt tcgagcatcc atggcgcggc
     3001 acggggcatc gccgttgccg agtggatcta cgacccggcg cgcccgccga ccgacgcacc
     3061 gatccgggcc cgaatcctca accagaccct gttcccgcag cgttcgttcg ggctcgcctc
     3121 gctggtcgac gacgacgggc gggcgatcgt ctacacctgc caggcacccg agcagggcgg
     3181 gatgcccgag cagtacgggc catgccacgc ggcgaaggtc gacctcgacc gggtggccga
     3241 tccgaccgcg tactcaacgt ggaccggcca ggacttcacc ggcaacgtga tgagctcgat
     3301 gccgctgtcg ttcgagcccg gcagcatcgc cgcgccactc ccggccggct cgttctcggt
     3361 cacgaaggat ccccatctcg gccggtacgt gatggtgtac agcccgtggc cgggctacag
     3421 cgactcgctg gtgatccgca cggcgaccgc accaaccggc ccgtggagcg ttccgaccgt
     3481 cgtgccgctc ccgaactgct ccgacgccgt gggcccgatc acgttctggt gctacgcggc
     3541 ctcggcacag cccatcttca gcgagcccgg caagctcggc gtcgggtact acgaccgcag
     3601 cgtgtcgacg ggaccggtgc gggggtcgta cctcgtggcc acggtcccct acgacgccgc
     3661 cgtgccggcc gggggctgac caggtgactc accggccgca gcgctgagcg gcgccgtcga
     3721 ttcgtcgggc cgcgaggcga cgatcagctc cagcatgcgg atgggtcgcg agacggtggg
     3781 cacctcgacg acatcgggga cggtgcgggt cgagccggca ccgtgctcgg acaacttgcg
     3841 cgccgtcgac acgacacgga ggtcgagcga gccgacggag cggttgaacg cgttgacggt
     3901 tcgatcgagc tggctgccga cggcggcgaa gtgctcggcg aagatggcga tgcgctcgtg
     3961 caactcgatg ccgagttgct ggatctcggc ggcggcgcgc tcggcctggt gctctcgcca
     4021 gcccaacgcg atgccacgga gcagcgggac gagcgacgac ggcgtgacga ggtggacgcc
     4081 cttggccgcc gcagcctcga agagcgtcgg gtcggcgtcg agtgcggcgg cgaggaacgg
     4141 ctcgcccgga aggaacagca ggaccatgtc gaccgagccg tcgaccttgg ccgcgtagtc
     4201 acgctgcgcc aacgccgtga catggccggc gacggccttg gcgtgctcgg cctggagccg
     4261 ccgctcgatg gcggggtcgc ccgcattggc ggcgtcgagg taccggtcga ggggcacttt
     4321 ggcgtcgatc acgacgcagc ggccgttggg gagtggcacg acgacatcgg gtcgcgagcg
     4381 ggactcgccg tcggtcacgc cccgctgctc gatgaagtcg gcgtggcggt cgagccccgc
     4441 catctcgagc acccgacgga gctgcacctc gccccacgcg ccgcgcacgc ggttgtcgcg
     4501 catggcagcc gcgagcttgg atgtctcggc ctgggtggcc cgatgggcga ccgacaggtc
     4561 gccgacgatg gcccgcagcg cgctcgtcgc gtcgttgcgc tcccgttcga gcgcgaccag
     4621 acgctccgac agcgcgcgga ggccgtcgcc gaccgccttg ctgtggcccg cgagcaccga
     4681 atcggtcgtc tggtggaggg cgccgtactt cgcctcggcg agttgcacga gttgggcgct
     4741 ctgcgctgca agcgcctcct gggagagtga gcgaaacgca tcgagcatct ggtcgtcggt
     4801 ggcggccgct gcctcgacac gggcaatgtg ctcgcgcagc agcgcctcgc gttcggcctg
     4861 acgattggcc cgatcgacgt ggaactcctc gcgccgggcg ctctcgcgag cagcatcggc
     4921 gagggccgag cgggcctgga cggcgagcca gattccgccg gcggaaccga ggagcagacc
     4981 gatgagcaag taggcgatgt ccatggaacg gaccgtaacg gcgaccactg acagcgagcc
     5041 gaggagcccg ttgcgtggcg ggttcgccgg accccaggcc cgtgcgaacg tggtcgatca
     5101 gaagggcgac tcgtcgcgcg ccgggtcgac cagactcgcc gggtcgaccg gcggtcgcac
     5161 cgacgcgagg ggcaccgacg gcacgtggat ccagccctcg agcaccgcgc agtggtggtc
     5221 ctcgaccgac tcgggcggga aggtctggtg ggcgcgagcg gcgagggcgt tgagcgccga
     5281 gagcaacgcg tcgagggtgt ggtcggagtc gatgcaggcc gaccgcaccc ggtcgagatc
     5341 gagccacgtg ctgcaatcgt cgacgatgcc ggcgaggatc gccttccgga cgcggcccgc
     5401 ctcggccgac tgccccttgt agccgcgctc gggcaggtgc cacgcccgga gggccgccgc
     5461 cggatacacc tcgaccaggc ggccgctgcc gtcacgcggc gcggcgccac cccaccgctc
     5521 cgcccagtcc cgctgcaaca gcgcgcagcg catggcgacg tgcccgagga ggtcggccga
     5581 cgccgacatc ggctgcccgc gtccgtggtc gcgcaccacg ttgtcggtgt ggcgatgggt
     5641 cagcgagcga cggtccttcg tgccgggcca cggctcgcgg tcctcatggg cccggacggc
     5701 ctgaacgaac gcgatgggcc acccgagcgg cgcgtccacc ccgatgagat cgacgtggcc
     5761 gccgagctcg acgaggtgtt cgtcggtggc gacctccggc gggaggtcgg cccgccaacg
     5821 accgggttcg accggatcga gcaccaccac accggtgttc cggggctgag cggccagatc
     5881 gatcccgagg atgcgcacac cgtgaaccta cgccggatcg tcgacggcgt ccgtcggggc
     5941 gccggcacat ggggcggtac catcgcggca cgaacgaggg ggcgtcgatg agcagcacaa
     6001 tcatccgggg tggcacggtc ttcgacggca cgggttccga cggccgccgc gccgacgtgc
     6061 tcatcgagaa cggtctcgtc gcgcgtatcg accacaacat cgaggtcggg cccgacgtcg
     6121 aggtggtcga cgccaacggg tgctgggtca cccccggctt catcgacctg cacacccact
     6181 acgacgctga actcgagatc gacccgtccc tcgtcgagtc ggtccgacac ggcatcacca
     6241 ccgtcctcgt cggatcgtgt ggactgtcgt tcgccgtcgg cgagcccgag gacctggccg
     6301 acatgttctg ccgggtcgag ggggtacccc gcgccgaggt gctcccgctc ctcgaacagg
     6361 tgcaggactg ggattcgccc aagggctacc tcgaccacct ggaacgtctt ccgctcggcc
     6421 cgaacgtcat ctcgtttctc ggccactccg cgatccgagc gcacgtcatg ggcctgggcc
     6481 gagcattgga cgacacggtg acgccgaccc ggtccgagct cgaccgcatg gcctcgatgc
     6541 tcgacgaggc cctcgacgtc gggtacctcg ggctctccat caacacgctg ccgtgggaca
     6601 agatggacgg cgatcggtac cgcagccggc cgaccccgtc ggtgttcgcg aactggaagg
     6661 agtaccgctt cctcggcgaa gtgcttcgcc gccgcgacgc cgtgctccaa ggcgtccccg
     6721 acctgtcggg ccgatggaac ttcgcgctct tcgtcgccat cgcgtcgggc atccgtcgca
     6781 agccgctccg cgtgtcgatc atctcgatgc tcgacgccaa ggcggcgccc ggagcgttca
     6841 aggtcatcgg cggggcgatg acgttcgccc gtcgctacct gcgggccgat gtgcgcctcc
     6901 agtcgctccc ccagccgttc cgcctctgga ccgacggcct cgagaacccg gtgatcgaag
     6961 agatcggagc cggcaccgag gcgctgcacc tggcgacgaa cgcacgggga gacctgctgc
     7021 gtgacccggt gtaccgggag cgcttccgca agcagtggag caacaagctc aagggccggg
     7081 cgtatcaccg cgatctcgcc gaggcccgca tcgtcgagtg ccccgacgac acgttggtcg
     7141 gcctcacgtt cgccgaggtt gccgtgcggc gcggccgcga ccggatcgac acgttcctcg
     7201 acctgcaggc cgagttcggc aacgacctgc ggtggacgac caccgtcggc aacaccaacg
     7261 aggagtccgt cgcctggatc gtgaaccatc cgcaggccca gatcgggttc tccgacgccg
     7321 gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcctcc gaccatcgcg
     7441 tcgggagggg gcgagaccat gagcgatcac cggcccggcc ccggatggcg tcgaggttcc
     7501 gaagcccagc gcgccacggc ggcggcgctc accgcgctcc cggcgcacac gcacttcttc
     7561 cacgggctgc cgatcggccg caccggtgac gacgtcgacc atctggtgat cgggccgacc
     7621 gggggtgtgg gcgatcgggt cgctccagtg gtcggggccg gcgaccacca caccggacgg
     7681 catgctgtgg ctgggcacca cgcccgtcag cgtcgaggcc gaccacatgt cgtcacgcgc
     7741 cgagtacgcc cgccacgcgt tggccggcga ggtgcggccg gtcctgtgcg tcgtcggagc
     7801 ccagttgcca caaccggtgc accggatcaa cggtgtggac gtcgtcgacg cggactggct
     7861 cgtgtcgttg ctcacgtccc acccgacgat cctctcgacc gtcgaggtcg acgctgccat
     7921 ccacgctgcg acgcggtggc gggtgcagcc tcccgaccac gttcccgagc ggtgggtgcc
     7981 gccgatcttc gagtcgtcga gggcgcttcc gccacctccg ccgcagcggt tcgaccggga
     8041 tccccgtcgt gtgtccgcct cgcaggtgat gatcgcggcg accgttgtcg ccgtgtcgat
     8101 gctcggcctc atcggctacc tcctcacggc cgaccggggc acggtgtcgg cgttcccaca
     8161 gaccgcgtcg atggcgtcgg gtcgagtcgt cgtgaccgac gtgccgggct acggcgcgct
     8221 ccgcgtcgag gtggtctgcc cggccgaggg cggccgtttc gatctcgtcg gccacgtgca
     8281 gggtgtgtac gacggtcggg ttcggatcgc gacgacggtg ggcggggaag gtcggtacct
     8341 cggcgagttc tcgagcctgc agcggacccc cgtgatcagc gtgttggcga ctgccaccga
     8401 caccacgttc tcggtccaac gcgtcgatcg ggcgggtcgc ggcggagagg tgtcgacgtt
     8461 cgccgtgacg acgccggccg agccgtgccg accggcgtcg gagcccaccg gagggtaggg
     8521 acaacccgac aacggacaca ccggctggcg ggatcggcag gcgacgggtc gattcctacg
     8581 ctcgtcccat gcgccgaatc gacctctccg ccgccccgca cgacgttcgc accgtcccgg
     8641 ccaggtcgtt cgccggacgg accgggaggt ggtggttggc ggtgacctcg ctggcgatcg
     8701 ccgcgatcgc tccactgccg tacctgacgt cgggcctcgg cgacatggcc cgggagggac
     8761 aggagatcgc cggcaactac gtcgatcggc cgggatggtt gcaggtcgcc ttctacgtgc
     8821 acgtgtgcct cggcggcctc gccatgctga cggcgccggt gcagctgtcc tcgcgggtcc
     8881 ggcggcgggc gccccgcctc caccgggtgg ccggtcgcgt cggcatcgcc tcgatcctgg
     8941 tcgcggcgat cgccggtgcc gtcctgtcgc cgtactcggt cgccgacggt gtcggcttcg
     9001 ccggattcgg gctgctcgcc gtcgcgtggt tcacatgcgc cctcgcctcg tatcgaacca
     9061 tccgccgcgg cgacgtcgcc gcgcaccgac gctgggcgat ccggacgttc gcgctcacct
     9121 acgcggccgt caccctccgc ctctggctcg gcgtgatgat cggcgtgcag gccggcatca
     9181 tgggcgtcga cggcgacctc gccttcgatc gggcgtacca catcgtcccg ttcctggcgt
     9241 gggtcccgaa cctgatcctc gccgaggtga tcatcgaccg gaccacg
//
LOCUS       DAOSOA010000106         9226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7443_length_9226_cov_6.281976, whole genome shotgun sequence.
ACCESSION   DAOSOA010000106 DAOSOA010000000
VERSION     DAOSOA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9226
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7443_length_9226_cov_6.281976"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKY13_08395"
     CDS             complement(<1..520)
                     /locus_tag="PKY13_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79993.1"
                     /translation="MTARLTARSGSSTGTRLRGGDLLRRSALVVGVLAVSVVAACVPE
                     EPPAPNPDVTVGAPSPTVVYGDGISVLHPTYTGNITPATPATCAADVSPASPPGTYPV
                     TCAGAADPGRTFIYENGYVTVVPAPLTVLSTSTSMTYGGTVPTIVAGYLGLKNGQTSP
                     AGAPTCSTTATSS"
     gene            complement(619..1380)
                     /locus_tag="PKY13_08400"
     CDS             complement(619..1380)
                     /locus_tag="PKY13_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT79994.1"
                     /translation="MRDAVGNINSVLVLGGASEIATETVQLLASRRCQLVVLAVRDPE
                     QVSDLLERYRALGMNAEAVAFDGAASATHQQVIDDVFAAHGDIDLVISAFGILGNQDD
                     FDADPVAAADAVVVNFAGQVSALTAVAAAMKRQGHGDIVVMSSVAGERVRKDNAVYGA
                     TKAGLDGYAQGLGDRLAGTGVRVLVVRPGFVHTKMTEGMEPAPFSTTPDKVAADIVAG
                     LDRGATTVWSPGVLRYLFTVMRHLPRPVWRIVSNR"
     assembly_gap    1725..1824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1825..2865
                     /locus_tag="PKY13_08405"
     CDS             <1825..2865
                     /locus_tag="PKY13_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT79995.1"
                     /translation="HQVHVAGTDGTETMLIGTSVILATGSVPRTIPGFEPDGRVVFTS
                     DEFLDLRTLPLRAAVIGGGAIGCEFASTLADMGTEVTILEGLPKILPGCDKDVAAIVE
                     RSFKKKGMTIRTGVSVTGHAPKADGGTVVSFGEETLEVDIVVVSVGRSPIARGVVAEG
                     PAVAVSERGFFEVDANCRTAEPGVWAIGDCIATPQLAHVGFIEGVNAIKDILGENPAA
                     VDYGKVPWAIYCHPEVSFAGHSEESAKEAGYEVVTNKHRFIGNGRAVILGETDGLVKV
                     IAEKRPDGSGGKILGVHMVGPWVTEQLGAGYFAVNWEATVDEVAELIQPHPSMSELFG
                     ETVIALTGRSLH"
     gene            complement(2882..3268)
                     /locus_tag="PKY13_08410"
     CDS             complement(2882..3268)
                     /locus_tag="PKY13_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT79996.1"
                     /translation="MNEERWETADQIQGALDRFVADVPGYRMPAVYAVGMVDDTGVAA
                     FPHVNRGDHPLPAAVLANVVGYRSGTRAHPLSPDELDRAIALLAPAEACRLVQHPNLW
                     AWREMRTRGGPDAAYVAIFVGDAELG"
     gene            3487..>4040
                     /locus_tag="PKY13_08415"
     CDS             3487..>4040
                     /locus_tag="PKY13_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOT79997.1"
                     /translation="MADIVMPQLGESVTEGTITKWFKQVGDHVAEDETLFEVSTDKVD
                     SEVPSPSAGYITEIKVPEGETVDVGTVLLVLADSPAGGAAAADAPAPVAEAPATEAPA
                     PAPEPPAPVAAPAPVAEAPTPPPAPAPAPAPAAAPAPAPAAAPEGSGAGGQLLSPLVR
                     RLIEENGLDATAIPGTGVGGRITR"
     assembly_gap    4041..4140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4141..4869
                     /locus_tag="PKY13_08420"
     CDS             <4141..4869
                     /locus_tag="PKY13_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOT79998.1"
                     /translation="PVLAGQGDSVVALSNIRLRTGEHMVRSLATSPHAITAIDVDFEA
                     VERARRAQKEQFKAEEGFSLTYLPFIARAVVDALAEFPHMNATVGERDLIVHHAVNLS
                     IAVDLNFEGLLAPVIQGADGKRLRAIAREVNDLATRARQKKLSPDDLNGGTFTISNSG
                     SYGTLLVAPIINQPQVGILSTDGVKRRPVVVELPDGSESIAIHSVGNLTLSWDHRAFD
                     GAYAAAFVARIKEILETRDWVAEL"
     gene            4869..6599
                     /gene="lipA"
                     /locus_tag="PKY13_08425"
     CDS             4869..6599
                     /gene="lipA"
                     /locus_tag="PKY13_08425"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOT79999.1"
                     /translation="MTLRVRWLGRVDYSDALALQRALCAGTDDYLLLLEHPHTYTMGV
                     RADAANILVDPHDVGARLVRADRGGDVTYHGPGQLVGYPILTLADHAAGEMPSTRAHV
                     LNIEHLVIEALADLGLPGASVIEGSPGVWLDADGHRPRKIAAVGVKISRGRTMHGFAI
                     NVDPDPAMFGHIVPCGITDKAVTSLRSEGVDVTMRQVVDAIVARAAVRFGDGDGDGVG
                     RDVDRADVVWRTSSTDADLAPFSRGEGAGTAVRYSSNTTQSDGTSLRLLGRLAEAGVS
                     ERVSITEPKPDWMRAKVRLDAEVLKVRSTLRDAGLVTVCEEAGCPNLSECWSDGTATF
                     MLCGERCTRACGFCLVDTSKPLPLDVDEPARVAQAVADMGLRFAVLTMVARDDLEDGG
                     AAHVAATIDAIHALGSAASPGPSVEALISDLRGDDRALDVVFAARPEVLNHNIETVAR
                     LQRAVRPSASYARSLSVLARAKAAGLTTKSGFVAGMGETFDEVVQTLADLASIGVEIV
                     TIGQYLRPTSHHLPVERWWTPTDFAEWKRIGEELGIGHVESSPLTRSSYHARSAAEGV
                     AATPVAIGAG"
     gene            6599..7159
                     /locus_tag="PKY13_08430"
     CDS             6599..7159
                     /locus_tag="PKY13_08430"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT80000.1"
                     /translation="MGKDYTALTEVHREWMTEQHLFFVATAPLAGDGHVNMSPKGYDT
                     FRVLDDHTVAYLDLTGSGIETIAHLREVPRLTIMFVAFEGRPRILRLYGRGSVVLHDE
                     PGFDRAAVPWTDVDGQLPLGSRAVVRLDIELVRSSCGYSVPFMAYGGERETLAEWATK
                     KGPDGIEQYWDDRNAVSIDGMPGLTA"
     gene            7176..8294
                     /locus_tag="PKY13_08435"
     CDS             7176..8294
                     /locus_tag="PKY13_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOT80001.1"
                     /translation="MTTSDAPFAARLGRVTARLDDLGVDALMVSVGPDLPWLTGYEAM
                     PLERLTMAVVTRDGRCTLVVPALEAPRVVARDHVEIVPWGESDDPIALVTERVGAATR
                     IAFGDRTWARFLVEIQDRLPDATFVRSNEVTGPLRAVKDGHEIAMLRRAGAAVDAIAA
                     DLQAGRIDLVGRTEAEVSADLGRRIVEAGHHRVNFAIVAAGANAASPHHEAGSRVIQE
                     GEVVLCDFGGTMFDEWGVGYCSDITRCVWTGPIPGEVADIYGVLHEAQAAGVAAGRVG
                     VACEDVDRACRDIIDAAGYGEFFIHRTGHGIGVEEHEDPYMVTGNTDVLAAGHCYSVE
                     PGIYLPGRLGFRLEDIVIATVEGPSPANNADHALAAIG"
     gene            8367..9224
                     /locus_tag="PKY13_08440"
     CDS             8367..9224
                     /locus_tag="PKY13_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80002.1"
                     /translation="MLNLDAATVLLQWSVGGLLFLWVTSRRREVGIGYGWLLRSVYGL
                     MAVGAAAAGFRYNRVWVREISSIAVAVACLGVLVVSWQRRKAGVLYQREQSQARSARV
                     AEMTGIEREEQTFDASVPEFPPILDLIAPVLGLVGLVAAGIDAGSPVWLQLFRVLIGT
                     AFLGCVTDAMLLGHWYLTQPGLPRRPLLELVRILTWLWPFELVAMLLPTGMVSVLNGS
                     IDDGWNGMLGWFWVACAITTIVLCRVTRAALKERAYSAVMAATGLLYLAILTGFGQDL
                     VARLVLNQP"
BASE COUNT         1309 a         3150 c         3153 g         1414 t
ORIGIN      
        1 tgctcgaggt cgccgtcgtg gaacacgtgg gggcgccggc agggctcgtc tgcccgttct
       61 tcaggccgag gtagccggcg acgatcgttg gcaccgtacc gccgtaggtc atggaggtcg
      121 aggtcgacag taccgtgagc ggggcgggca cgaccgtgac gtacccgttc tcgtagatga
      181 acgttcggcc ggggtcggcg gcgcccgcac acgtgacggg gtaggtgccg ggagggctgg
      241 ccggcgacac gtcggcagcg cacgtcgccg gggtcgccgg cgtgatgttg ccggtgtagg
      301 tggggtgcag caccgagatc ccgtcgccgt acacgaccgt cggcgacggg gctccgacgg
      361 tgacgtcggg gttcggcgcc ggtggctcct ccggaacgca cgccgctacg acgctcacgg
      421 ccaacacccc gacgacgagc gccgagcgtc ggagcaggtc gccgccgcga aggcgcgtcc
      481 ccgtggacga cccggatcgc gcagtcagtc gtgcagtcat gcccacccct tgcatgccga
      541 cggagatcga gcccgctgat cgggcgccgt tccgccggcg agccctcgtg aaccgtatcc
      601 tgtccgcccg aacggtgctc agcggttcga gacgatgcgc cacaccgggc gcggcaggtg
      661 ccgcatcacg gtgaacaggt accgcagcac ccccggcgac cacaccgtcg tcgcgccgcg
      721 atcgagcccg gcgacgatgt cggccgcgac cttgtcgggg gtcgtcgaga acggcgccgg
      781 ctccatgccc tcggtcatct tcgtgtgcac gaacccgggg cggaccacca acacccgcac
      841 gccggttccg gcgaggcggt cgccgagacc ctgggcgtaa ccgtcgaggc cggccttcgt
      901 cgccccgtac acggcgttgt ccttgcgtac ccgctcgcca gcgacggacg acatgacgac
      961 gatgtcgccg tgcccctgcc gcttcatggc ggcggcgacc gcggtgagcg ccgacacctg
     1021 gccggcgaag ttgacgacca cggcgtcggc cgctgcgacg ggatcggcgt cgaagtcgtc
     1081 ctggttgccg aggatgccga acgccgagat caccaggtcg atgtcgccgt gggcggcgaa
     1141 cacgtcgtcg atcacctgtt ggtgggtcgc gctggcggcg ccgtcgaacg ccacggcctc
     1201 ggcgttcatg ccgagggccc ggtaccgctc gaggaggtcc gacacctgct cgggatcacg
     1261 caccgcgagc acgacgagct ggcaccggcg gctggccagg agctgcacgg tctcggtcgc
     1321 gatctccgag gcgccgccca gaaccagcac cgagttgatg ttgccgactg cgtcgcgcat
     1381 ggagctcctg ttcagaacgc ggaccgtgaa tgggaccgaa cgacgggttc accgtatcct
     1441 ggctgcccgt gagcacccga atgctgatca ccctcgccgt catctgcggt gtcctcatcc
     1501 tggcggctgc gggcctccag ttcgctctgg tcaaatagct gtcggcaagt agctgtcggc
     1561 aggcgactgc cgacgtcgct cgcgggaaca ccgccacccg gccgcgggtt taggatcagc
     1621 gagaccaccg attgctcgca aggacctccc gtggaacaga accccccagt caagtacgac
     1681 gtcgtgatca tcggtggtgg cccgggtggc tacgccgctg cgctnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnncaccag gtgcacgtcg ccggcaccga cggcaccgag
     1861 acgatgctga tcggcacgtc ggtgatcctg gcgacggggt cggtgccacg cacgattccc
     1921 ggcttcgaac ccgacggtcg cgtcgtcttc acgagcgacg agttcctcga cctccgcacg
     1981 ctgcccctcc gcgccgccgt catcggcggt ggcgccatcg gctgcgagtt cgcgtcgacg
     2041 ttggccgaca tgggcaccga ggtcacgatc ctcgaggggc tgccgaagat ccttcccggg
     2101 tgcgacaagg acgtggctgc gatcgtcgag cggtcgttca agaagaaggg catgacgatc
     2161 cgcaccggcg tgtcggtcac cggccatgcc cccaaggccg acggcggcac cgtcgtgtcg
     2221 ttcggcgagg agaccctcga ggtcgacatc gtggtggtgt cggtcggtcg gtcgcccatc
     2281 gcccgcggcg tggtcgccga gggcccggcg gtcgccgtga gcgaacgtgg cttcttcgag
     2341 gtcgacgcga actgccgcac ggccgagccc ggcgtgtggg caatcggcga ctgcatcgcg
     2401 acgccccagc tggcccacgt cggcttcatc gagggcgtca acgcgatcaa ggacatcctc
     2461 ggcgagaacc cggcggcggt cgactacggc aaggtgccgt gggcgatcta ctgccacccc
     2521 gaggtcagct tcgccggcca ctccgaggag tcggccaagg aggccgggta cgaggtcgtc
     2581 accaacaagc accgcttcat cggcaacggc cgtgcggtga tcctgggcga gaccgacggc
     2641 ctcgtcaagg tcatcgcgga gaagcgaccc gacggcagcg gcggcaagat cctcggtgtg
     2701 cacatggtcg gcccgtgggt caccgagcag ctgggcgcgg gctacttcgc ggtcaactgg
     2761 gaggcgaccg tcgacgaggt ggccgagctg atccagccgc acccgtcgat gagtgagctc
     2821 ttcggcgaga ccgtgatcgc gctcacgggt cgttcgctgc actgagcctc gtcgtcgggt
     2881 gtcagccgag ttcggcgtca cccacgaaga tggcgacgta ggccgcgtcg gggccgccgc
     2941 gggttcgcat ctcgcgccag gcccacaggt tggggtgttg gacgagccga cacgcctccg
     3001 ccggggccag gagcgcgatc gcacgatcga gttcgtcagg cgagagcggg tgtgcccggg
     3061 tgcccgagcg gtacccgacg acgttggcga gcacggcggc cggcagcggg tggtcgccac
     3121 ggttcacgtg gggaaaggcc gcgacgccgg tgtcgtcgac catgccaacg gcgtacaccg
     3181 ccggcatgcg gtatcccggc acgtcggcga cgaagcggtc cagcgctcct tggatctggt
     3241 ccgccgtctc ccagcgctcc tcgttcatcg agggatcaca acagattcgg ccgtgctgcg
     3301 cactgagaaa ttcggaccct gttcgacgca tgggaacaca tcggccggtg gtggggtttt
     3361 cgtgggcgat ggtgccgcta gcgtggtgga atacaggttg catacatgtt cgggaggcgc
     3421 cgcactggtt ccccgtgacg agcgacaggc tcgtcaccgc ctcccgtcga gaagggacgt
     3481 tcagcgatgg ctgacatcgt gatgccgcag ctcggagaat cggtcaccga gggcacgatc
     3541 accaagtggt tcaagcaggt cggcgaccat gtcgccgagg acgagacgct gttcgaggtc
     3601 tccaccgaca aggtcgattc cgaggtcccc tcgccgtccg ccgggtacat caccgagatc
     3661 aaggtgcccg agggtgagac ggtcgacgtc ggcaccgtgc tcctggtcct ggccgactcg
     3721 ccggccggcg gagccgctgc cgccgatgca ccggctcccg tcgctgaggc gcccgcaacc
     3781 gaggcgcccg cgcctgcacc cgagccgccc gccccggttg ccgccccggc gccagtggcc
     3841 gaggccccga cgccacctcc cgcccccgcc cctgccccgg caccggccgc ggctcccgcc
     3901 ccggcaccgg ctgcggcgcc cgaaggctcc ggtgccggtg gccagctgtt gtccccgctc
     3961 gtccgtcgcc tgatcgagga gaacgggctc gacgccaccg ccatccccgg caccggtgtc
     4021 gggggccgga tcactcgtga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 ccggtcctcg ccggtcaggg cgactcggtc gtggcactga gcaacatccg cctgcgcacc
     4201 ggcgagcaca tggttcgttc gttggccacg tcgccgcacg cgatcacggc gatcgacgtc
     4261 gacttcgagg ccgtggagcg tgcccgccgt gcccagaagg aacagttcaa ggcagaggag
     4321 ggcttctccc tcacctacct gcccttcatc gcccgggccg tcgtcgacgc actcgccgag
     4381 ttcccgcaca tgaacgccac ggtgggagag cgcgacctga tcgtgcacca cgccgtcaac
     4441 ctgtcgatcg ccgtcgacct caacttcgag ggcctgttgg ccccggtcat ccagggcgcc
     4501 gacggcaagc ggctccgcgc catcgcccgc gaggtcaacg atctggccac ccgtgcccgt
     4561 cagaagaagc tctcgcccga cgatctcaac ggtgggacgt tcacgatctc gaactccggt
     4621 agctacggca cgctgctcgt ggccccgatc atcaaccagc cgcaggtggg catcctgtcg
     4681 accgacggtg tcaagcgccg cccggtcgtc gtcgagttgc ccgacggctc ggaatcgatc
     4741 gccatccact cggtcggcaa cctgacgctc agctgggacc accgggcctt cgacggcgcg
     4801 tacgccgcgg cgttcgtcgc acggatcaag gagatcctcg agacccgcga ctgggtggcg
     4861 gaactgtagt gaccctgcgc gtccgctggt tgggtcgcgt cgactacagc gacgcgctcg
     4921 cgctccagcg ggccctgtgc gccgggacgg acgactacct cctcctgctg gagcacccgc
     4981 acacctacac gatgggggtg cgggccgacg cggccaacat cctcgtcgac ccccacgacg
     5041 tcggggcccg actggttcgg gccgatcgag gtggcgacgt gacgtatcac gggccgggtc
     5101 agttggtggg gtatccgatt ctcaccctgg ccgatcacgc tgccggtgag atgccgtcga
     5161 cgcgggccca cgtcctcaac atcgagcacc tggtcatcga ggcgctcgcc gacctcggtc
     5221 tgcctggcgc ctcggtcatc gaggggtcac cgggggtgtg gctcgacgcc gacggccatc
     5281 ggccccgcaa gatcgcagcg gtcggggtga agatcagccg cgggcgcacc atgcacggct
     5341 tcgcgatcaa cgtcgatccc gatccggcca tgttcggcca catcgtgccg tgcggcatca
     5401 ccgacaaggc ggttacgtcg ctccgttccg agggcgtcga cgtgacgatg cgacaggtcg
     5461 tcgacgcgat cgtggcccgg gcggcggttc ggttcggcga tggcgatggc gatggcgtgg
     5521 gccgcgatgt cgaccgcgcc gatgtggtgt ggcgcacgtc gtcgaccgac gcagacctgg
     5581 cgccgttcag ccggggcgag ggcgcgggca cggcggttcg ctactcgtcg aacaccacgc
     5641 agtccgacgg cacgagcctt cggttgctgg gacgcctcgc cgaggccggc gtcagcgagc
     5701 gggtgtcgat caccgagccg aagcccgact ggatgcgggc caaggtccgc ctcgacgccg
     5761 aggtcctgaa ggtgcggtcg acactgcgcg acgccggcct cgtgacggtg tgcgaggaag
     5821 cagggtgccc gaacctcagc gagtgctggt cggacggcac tgccaccttc atgctgtgtg
     5881 gcgagcgatg cacccgggcc tgcgggttct gcctcgtcga cacgtccaag ccgctgccgc
     5941 tcgacgtcga cgagccggcg cgggtggctc aggcggtcgc cgacatggga ctgaggttcg
     6001 ccgtactcac gatggtcgcc cgcgacgacc tcgaggacgg cggcgcggcc cacgttgcgg
     6061 ccaccatcga cgcgatccac gcgctcggct ccgcagcgtc gccggggccc tcggtcgagg
     6121 cgctcatcag tgacctgcgc ggcgacgacc gagccctcga cgtcgtcttc gcggcccggc
     6181 ccgaggtcct gaaccacaac atcgagacgg tcgcccgcct ccagcgggcg gtgcgtcctt
     6241 cggcttcgta cgcccgcagt ctctcggtcc tcgcccgggc gaaggcggca ggactcacga
     6301 ccaagtcggg gttcgtcgcc ggaatgggcg agacgttcga cgaggtcgtc cagacgctcg
     6361 ccgatctggc ctcgatcggc gtcgagatcg tcacgatcgg ccagtacctc cgtcccacct
     6421 cccaccacct gccggtcgag cggtggtgga cgccgaccga cttcgccgaa tggaagcgca
     6481 tcggtgagga gctcggcatc ggtcacgtcg agtcgtcgcc gctgacccgg tcgagctatc
     6541 acgctcggag cgctgccgag ggcgttgcag cgacgcccgt cgccatcgga gccgggtagt
     6601 gggcaaggac tacacggcgc tcaccgaggt gcaccgggag tggatgaccg agcagcacct
     6661 gttcttcgtg gccacggccc cgctcgctgg ggacggccac gtcaacatgt ccccgaaggg
     6721 ctacgacacg ttccgggtgc tcgacgacca caccgtcgcg tacctcgacc tcaccggttc
     6781 gggtatcgag acgatcgccc acctccgcga ggttccccgg ctgacgatca tgttcgtcgc
     6841 gttcgagggt cgcccccgca tccttcgtct gtacggccgc ggttcggtgg tgctccacga
     6901 cgaaccaggg ttcgaccggg ccgcggtgcc gtggaccgat gtcgacggcc aactgcctct
     6961 gggctccaga gcggtcgtgc ggctcgacat cgagctcgtg cgcagctcct gcgggtactc
     7021 cgtgccgttc atggcgtacg gcggggaacg cgagacgctg gcggagtggg cgaccaagaa
     7081 gggtcccgac gggatcgagc agtactggga cgaccgcaac gccgtgtcga tcgacggcat
     7141 gcccggcctg acggcctgaa gggaacacac cgaacatgac aacttccgac gcgccgttcg
     7201 cagcccgact cggtcgggtg acggcccgac tcgacgacct cggtgtggac gcgctgatgg
     7261 tgtcggtcgg acccgacctg ccgtggctga ccggctacga ggcgatgccg ctcgaacgtc
     7321 tcacgatggc cgtcgtgacc cgtgacggcc gctgcaccct cgtcgtgccg gccctggaag
     7381 cgccccgtgt cgtcgcccgc gaccatgtcg agatcgtgcc gtggggcgaa tccgacgacc
     7441 cgatcgccct ggtcaccgag cgggtcgggg cggcgactcg gatcgccttc ggcgaccgga
     7501 cctgggcccg gttcctcgtc gagatccagg accgtctccc tgacgccacg ttcgtacggt
     7561 ccaacgaggt caccggcccg ctgcgcgcgg tgaaggacgg ccacgagatc gcgatgcttc
     7621 gccgagcggg ggccgccgtc gacgcgatcg ccgccgacct gcaggccggc cgcatcgatc
     7681 tcgtcggacg aaccgaagcc gaggtgtcgg ccgatctcgg tcgtcgcatt gtcgaggccg
     7741 gccaccaccg ggtgaacttc gccatcgtcg cggcgggtgc caacgcggcg agcccccatc
     7801 acgaggcggg gagtcgggtc atccaggaag gtgaggtcgt gctgtgcgac ttcgggggca
     7861 cgatgttcga cgagtggggc gtgggctact gctccgacat cacccggtgt gtctggaccg
     7921 ggccgattcc cggtgaggtg gccgacatct acggcgtgtt gcacgaggcg caggccgccg
     7981 gggtcgccgc cggccgggtc ggcgtcgcct gcgaggacgt cgaccgcgcg tgccgcgaca
     8041 tcatcgacgc tgccgggtac ggcgagttct tcatccatcg caccggccac ggcatcggcg
     8101 tcgaggagca cgaggacccg tacatggtca ccggcaacac cgacgtgctc gccgcgggcc
     8161 actgctattc cgtcgagccg ggcatctacc tgccgggccg gctcggcttc cggctggagg
     8221 acatcgtcat cgcaaccgtc gagggcccgt cgccggccaa caacgccgat cacgccctcg
     8281 cagcgatcgg ttgacgcagc tgcgtcggtg gtcgatcgcg gtcgttttcc gtccggaacg
     8341 cccgacgtcc tagcctgcct gccgtcatgt tgaacctcga cgccgccacc gtgctgctgc
     8401 agtggtcggt cggtgggctg ctcttcctct gggtcacgtc gcgtcgccgc gaggtcggca
     8461 tcggctacgg ctggctcctg cgctcggtct acgggttgat ggcggtcggc gccgccgccg
     8521 ccggcttccg atacaaccgg gtctgggtgc gcgagatctc gtcgatcgct gtggccgtgg
     8581 cctgcctcgg ggtgctcgtc gtcagctggc agcggcgcaa ggccggggtg ctctaccagc
     8641 gggagcagtc gcaggcgcgg tccgctcgcg tcgccgagat gaccggcatc gagcgggagg
     8701 agcagacgtt cgacgcctcg gtgcccgagt tcccgccgat cctcgacctc attgccccgg
     8761 tcctcgggct cgtgggcctc gtcgccgctg gcatcgacgc cggcagcccg gtctggctcc
     8821 agctcttccg ggtgctgatc ggcaccgcgt tcctcggctg cgtcaccgac gccatgctgc
     8881 tggggcactg gtacctcacg cagccgggtc tgccgcggcg gccgctcctc gaactcgtcc
     8941 gcatcctcac gtggctctgg ccgttcgagc tcgtggcgat gttgctgccg accggcatgg
     9001 tgtcggtgct gaacggctcg atcgacgacg gctggaacgg gatgctcggg tggttctggg
     9061 tggcgtgcgc gatcacgacg atcgtcctgt gccgcgtcac ccgcgccgcg ctcaaggagc
     9121 gtgcctactc ggccgtgatg gcggccaccg ggctcttgta cctcgcgatc ctgacggggt
     9181 tcggtcagga cctcgtcgcc cgcctcgtcc tcaatcagcc ttgatg
//
LOCUS       DAOSOA010000107         9210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7460_length_9210_cov_5.784162, whole genome shotgun sequence.
ACCESSION   DAOSOA010000107 DAOSOA010000000
VERSION     DAOSOA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7460_length_9210_cov_5.784162"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKY13_08445"
     CDS             <1..219
                     /locus_tag="PKY13_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80003.1"
                     /translation="REGIGNPRPEAEGIENQDQLAMLRNGGCDIGQGYYLARPLPAAA
                     VREMLVVSRAADRRDRNIVLPVLPVASA"
     gene            complement(247..2484)
                     /locus_tag="PKY13_08450"
     CDS             complement(247..2484)
                     /locus_tag="PKY13_08450"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT80004.1"
                     /translation="MLLVIVAGALGGGVFGRLSGGGFEDPAADSSRAEAALQDVFDTG
                     VPNYLLLVDANEGAEGATPPAVDDASVSDAATAIETRLRNDPAVAEVLSYWSLGKVAP
                     LRSDDGTAALIVARLGGDETEQQEAAERLDGALLGTDGPITVGSGGRSPIFQSVSSQI
                     EGDLAGAESIAVPITLVLLVLVFGGLVAAGLPLVVGAVSVLGTFFTLWVITQFTDVSI
                     FSINLVTALGLGLAIDYSLFIVSRFREEIRAGRSVEAAIIRTVETAGRTIAVSALTVA
                     VSLSALLVFPLYFLRSFAYAGVGVTLVAMATSVITLPALMAVLGHRVDSLRLWKRTEP
                     KPVGDGMWHTIATVVMKRPLVIGAAVVALLVFLGTPFFGVEFGLPDQRVLPEDTPARI
                     TTERIADDFSSEESSAMPVVALDAADPDDTTIDAYAVALSSVPGVTRVDAATGRFIDG
                     TKVIGPDPSLAGFRSAETGAVRFSVVPDVVPISTEAETLVHDLRDVESPIGRTLVGGQ
                     SAALVDTKASIADRLPLAAGIIAGATFVLLFLMFGSVLVPLKAIVLNVLSLSATFGAM
                     VWIFQDGHGAGLLGFTATGLTDTTTPILMFCIAFGLSMDYEVFLLSRIKEEHDRTGDN
                     AASVAMGLERTGRIVTAAAALLAVTFIAFATSGISFIKLFGLGLTLAVVMDATLIRAT
                     LVPAFMKLAGEANWWAPRWMRWIHDRIGFSESDPPEPPEPDAAGLDLTLDPIDLSGDR
                     EQAVV"
     gene            2617..3297
                     /locus_tag="PKY13_08455"
     CDS             2617..3297
                     /locus_tag="PKY13_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT80005.1"
                     /translation="MSSVTPRPRNARGQGAQLRDDLLDATLAGLAVTGDPEDVSIRGV
                     AKTTGVSPTAVYQHFTDRDALIEAACDRAFDHFAAYLLAAVEPAVDAFDRLRLAGHAY
                     LRYAVDEPGLYRVLFSNPLHLALHTDETKAVIPGAPGAPGTPGVSAFGVLVDMVQACL
                     DAGAPASTSSGTAPDATYLSFQVWTWLHGIVDLRITHPAMPWPPAEPMVDDIARVLGL
                     DSPRVAPR"
     gene            complement(3400..4227)
                     /locus_tag="PKY13_08460"
     CDS             complement(3400..4227)
                     /locus_tag="PKY13_08460"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOT80006.1"
                     /translation="MPPSARTDAGKSQRVAELADALENVQAARPIAMQVLQLADDPDA
                     DARKVAACIEQDPILVAQVLRLANSAAFGMARQVKTTQIAVAILGFSTIRSMAILVAS
                     GLRNLKHPAPAGFWEHSAATAAACSVISRQFGVSPGDGFAIGLIHDIGIPMLNTVDPV
                     AFAGISSVDDAAACELEADLFGLSHAEAAAMVLSNWSFPESLVGAIGSHHLDAPLETN
                     ESRLLFAGDAIAHLASDDGSKADRRRLRSLGFEKSEIDRLVGDVGGRSGGILASLLR"
     gene            complement(4349..5308)
                     /locus_tag="PKY13_08465"
     CDS             complement(4349..5308)
                     /locus_tag="PKY13_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HOT80007.1"
                     /translation="MFQTLTPSITHRAVPVVPVSWTAPVAPTDGPTTPAFVRFAARSA
                     RTLPDAAYDALVDLGDDLDGVGAVVLRGLPVGRVPATPPHPAAPTDKDTTSELTLLTV
                     ARLLGQPVGYLPEHGGDLVQNIVPTSSDVARQTSTSSRVQLAFHTETAFHPHKPRFLV
                     LLCLKGDPAASTTLCSIDDILPGLDTRQRQVLAEPRFHTRADESFGGGVDARFLPPMR
                     VLTGDPDHPTLTFDAELTTGLDDEAQAALDALTALVETHHTSLVLEAGDLLVVDNHRC
                     VHGRSPFAARFDGTDRWLQRSFVVADLSASADDRVGRIITTTF"
     gene            complement(5377..6228)
                     /locus_tag="PKY13_08470"
     CDS             complement(5377..6228)
                     /locus_tag="PKY13_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOT80008.1"
                     /translation="MGDVFDRIHAAADRLTAAERKVAASITDDPQAVAFGTVAEIASR
                     SGSSGASVVRLATKLGYRGFSDLQAAVQAELGRRLRPATERIHDPHPGDLRGQGLAAA
                     LECVQATLAGVESADLDAIVKVLSIRSRPVWVVAGDASSGIARQFATELSMLRPSVVE
                     LTGSPVNVARQLADIDSGDVVVALDLRRYDRWVLDAVAAATAAGATLIALTDGPLSPL
                     ASGAAHVISIEADGIGPFDNYVGALAALATITAGVAVRLRAPAAAHLERIEGAWRRSG
                     ALVDPQD"
     gene            complement(6238..7545)
                     /locus_tag="PKY13_08475"
     CDS             complement(6238..7545)
                     /locus_tag="PKY13_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOT80009.1"
                     /translation="MPERQPTRRLSLTEAAALFDPVDSLGLPLGPGQPVGLVHALGER
                     SDWQRFDVLGAMLVDFYALFDHPGVFLTSTFFGPAERIYRDMGRNIAYVPSDFRRFAT
                     VMEARRPRVIATMATAPDADGWMSLSLHAGASVGELHRAAADPDRLLIVEASPHFPRT
                     AGITPEGHRLHVDEVDVVCESDRRPVELVDPEVTEVELAIAAHAQRFIPDGATLQTGF
                     GSIPSTIASVLAHGDGGEYGVHSEMFTTGLMRLHQAGKVTNVHKDQFEGYSLVTFAAG
                     TAELYAWLDGRDDVRFAPVSVVNSPERIAANHTMVTINGAIAVDLWGQVTADSIGGLQ
                     FSGVGGHEDFVSGSGLELEDRSLICLPATVEVGGVLRSRIEPTFGPGTIVTTPRHQID
                     TIITEFGAAEVRGLSTSDRARALAGIAHPAFRDELLAAAPTGP"
     gene            complement(7646..>9210)
                     /locus_tag="PKY13_08480"
     CDS             complement(7646..>9210)
                     /locus_tag="PKY13_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOT80010.1"
                     /translation="RCHLGTVTDTRKASSAAATGNCDGNSRPQVELSCPTSVLYGEPI
                     SFTAIGIDDDGDPLTYLWKINGTAVVGQTGATATLTIQAGDRINVTAIDDSETASAST
                     TIQCTGTSRPTVTITCPAKLVYGEPMIFIANGLDPDGDTKLAYSWYVNGTPVPKESSP
                     KLTATVAKGDVLTVTVTDSEGAVSASAAADCAGNSRPTVALVCPEKLVYGQPTTFTAT
                     GTDADGDELTYEWKIGDDVVKGQTGATAELTVAKGDRVTVTATDSKGATSTAATSTCD
                     GDSTPTVTTTCPARVVWGEPVVFEAVGKDDDGDTLTYAWTVNGTPVAGQTAARVTLTL
                     AKGDVVTVTATDPTGLASAAATFDCTGKERPKISVTCPADFTFGQPTKLVATSTNAKV
                     ADLVFTWAVNGKVVDGTTSQITLTLRPDDVIGVSATDTDGLVAVTATTTCIGVTEPPR
                     TPVPPTPSMPDVLGSTVYKAATQSSRPSSGSLPVTGGEAAAMALVALVALGSGLVLVR
                     FSRRKGRRPEQS"
BASE COUNT         1417 a         3049 c         3446 g         1298 t
ORIGIN      
        1 agggagggca tcggtaatcc ccgccctgag gctgagggca tcgagaacca ggaccagctg
       61 gcgatgctcc gcaacggcgg ttgcgacatc gggcagggct actacctggc ccggccgctg
      121 cccgccgctg cggtgcgcga gatgctcgtc gtcagccggg cagccgatcg ccgtgaccgc
      181 aacatcgtgt tgcccgtgtt gcccgtggcg agcgcgtgat cggtaccacg ggcgggggac
      241 cgcccgtcag acgactgcct gctcccggtc gccggagagg tcgatcgggt ccagcgtgag
      301 atcgaggccg gcagcgtcgg gttccggagg ctccggaggg tccgactccg agaacccgat
      361 ccggtcgtgg atccaccgca tccaccgcgg cgcccaccag ttggcctcgc cggcgagctt
      421 catgaacgcc ggcaccagcg ttgcccggat cagcgtcgcg tccatcacga cggcgagggt
      481 gaggccgagg ccgaacagct tgatgaacga gatgcccgag gtggcgaagg cgatgaacgt
      541 gacggcgagc agtgccgctg cggccgtgac gatgcgtccg gtgcgctcga gacccatcgc
      601 caccgaggcg gcgttgtcgc cggtgcggtc gtgctcctcc ttgattcggg agagcaggaa
      661 gacctcgtag tccatcgaga ggccgaaggc gatgcagaac atgaggatcg gcgtcgtcgt
      721 gtcggtcagg ccggtcgccg tgaacccgag cagcccggca ccgtgaccgt cctggaagat
      781 ccacaccatt gccccgaacg tcgcactcag ggacaggacg ttcagcacga tcgccttgag
      841 tggcacgagc accgacccga acatcaggaa cagcaggacg aaggtggccc cggcgatgat
      901 cccggccgcc agcggcagac ggtcggcgat cgacgccttg gtgtcgacga gggccgccga
      961 ctggccgccg acgagggtcc gaccgatcgg cgactcgacg tcgcggaggt cgtggacgag
     1021 cgtctcggcc tccgtcgaga tcggcaccac gtcgggaacg acgctgaacc ggaccgcacc
     1081 cgtctcggcc gatcggaagc cggcgagcga cgggtcgggc ccgatcacct tggtgccgtc
     1141 gatgaaccgc ccggtggcgg cgtcgacccg ggtcaccccg gggaccgacg acagcgcgac
     1201 cgcgtaggcg tcgatcgtcg tgtcgtcggg atcggccgcg tcgagcgcga cgacgggcat
     1261 ggccgacgat tcctccgacg agaagtcgtc ggcgatgcgc tcggtggtga tgcgggcggg
     1321 cgtgtcctcg ggcagtaccc gctggtcggg cagaccgaat tccaccccga agaagggggt
     1381 gcccaggaac acgaggaggg cgaccacggc ggcgccgatc acgaggggcc gcttcatgac
     1441 gaccgtggcg atggtgtgcc acatgccgtc tccgaccggc ttgggctcgg tccgcttcca
     1501 gaggcgcagc gagtcgaccc ggtgaccgag gaccgccatg agcgccggca gtgtgatgac
     1561 gctcgtggcc atcgccacga gcgtgacgcc gacgcccgcg taggcgaacg agcggaggaa
     1621 gtagagcggg aacacgagga gcgccgagag cgacacggcg acggtgagcg ctgagacggc
     1681 gatcgtcctg ccggccgtct cgacggtgcg gatgatcgcc gcctcgaccg accggccggc
     1741 gcggatctcc tcgcggaacc tcgacacgat gaacagcgag tagtcgattg ccaggccgag
     1801 ccccaatgcg gtgacgaggt tgatcgagaa gatcgagacg tcggtgaact gggtgatcac
     1861 ccacagggtg aagaacgtgc cgagcaccga gacggcaccg acgacgagcg ggagcccggc
     1921 ggcgacgagg ccgccgaaca cgaggacgag caggacgagc gtgatcggta cggcgatgct
     1981 ctcggcgccg gcgagatcgc cttcgatctg gctcgacacc gactggaaga tcggagagcg
     2041 gccgcccgac ccgaccgtga tcggtccgtc cgttccgagc agtgcgccgt cgagtcgttc
     2101 cgctgcctcc tgctgctcgg tttcgtcgcc gccgaggcgg gcgacgatga gcgctgcggt
     2161 gccgtcgtcg gatcgcaggg gagcgacctt gccgagcgac cagtagctca gtacctccgc
     2221 cacggcgggg tcgttgcgga ggcgggtctc gatggccgtg gccgcatcgg agaccgacgc
     2281 gtcgtcgacg gcgggcggcg tggctccttc ggcgccctca ttggcgtcga cgaggaggag
     2341 gtagttgggg acacccgtgt cgaacacgtc ctgcagggcc gcctcggcgc gggacgaatc
     2401 ggcggccgga tcctcgaacc cgccgcccga caggcggccg aacactccgc cgccgagtgc
     2461 gccggcgacg atgaccaaca gcacggatgc gacgaggatg gctcggcggc ggcgtacggt
     2521 gaaacttcca agtctgctga acatcgatcg atccgtcccc cggtagcgtt ttgttaacgg
     2581 gtgtacatat atgcctgtca acctaggatg agcaacatgt catcggtcac accccgacct
     2641 cgaaatgccc gcggccaggg cgcacaactc cgcgacgacc tcctcgacgc gaccctcgcc
     2701 gggctcgccg tcaccggcga ccccgaggac gtctcgatcc gcggcgtggc caagaccacc
     2761 ggcgtctcac cgaccgcggt gtaccagcac ttcaccgacc gcgacgccct gatcgaggcc
     2821 gcgtgcgacc gggcgttcga ccacttcgcg gcgtacctgc tcgccgcggt cgagcccgcc
     2881 gtcgacgcgt tcgaccgcct gcgcctggcc ggtcacgcct acctccgcta cgccgtcgac
     2941 gagccgggcc tctaccgggt gttgttcagc aatccactgc acctcgccct gcacaccgac
     3001 gagacgaagg cggtcatccc cggcgcgccg ggcgcgccgg ggacgcctgg cgtctcggcg
     3061 ttcggggtgc tcgtcgacat ggtgcaggcc tgcctcgacg ccggggcacc ggcgtccacg
     3121 tcgtcgggca ccgctcccga cgcgacgtac ttgtcgttcc aggtatggac ctggctgcac
     3181 ggcatcgtcg acctgcgcat cacgcacccc gccatgccat ggccgccggc cgaaccgatg
     3241 gtcgacgaca tcgcccgggt gctgggtctc gacagccccc gagtcgctcc gagatgagag
     3301 ttccgagcgt ggcacctgct cgggaagcgt cgcgttcctg aggatggtgc tcgtgcggtc
     3361 cgattcgggc gcgctggcgc accatcctcg caacgacggc tagcgcaaca ggctcgccaa
     3421 gatgccgccc gagcggccgc ccacgtcacc aacgaggcgg tcgatctcgg atttctcgaa
     3481 gccgagcgac cggagccgtc ggcggtccgc cttcgacccg tcgtccgacg cgaggtgcgc
     3541 gatggcgtcg ccggcgaaga gcaggcggga ctcgttcgtc tcgagcggcg cgtcgaggtg
     3601 gtgcgaaccg attgcgccga cgagcgactc gggaaacgac cagttggaga gcaccatggc
     3661 cgcggcctcg gcgtggctga gtccgaagag gtccgcctcg agttcacacg ccgcggcgtc
     3721 gtcgaccgat gagatgccgg cgaaggccac cggatcgacg gtgttgagca tcgggatgcc
     3781 gatgtcgtgg atgagtccga tcgcgaatcc gtcgccgggt gagacgccga actggcggct
     3841 gatcaccgag cacgcagcgg ccgtcgctgc ggagtgctcc cagaacccgg ccggggcagg
     3901 atgcttcagg ttgcggaggc ccgaggcgac gagaatcgcc atcgatcgga tcgtcgagaa
     3961 gccgaggatc gcgacggcga tctgcgtcgt cttcacctgg cgggccatac cgaacgcggc
     4021 cgagttggcc aaccggagga cctgcgccac gaggatcgga tcctgctcga tgcacgctgc
     4081 gaccttgcgg gcgtcggcgt cggggtcgtc ggccagttgc agcacctgca tggcgatggg
     4141 gcgggccgcc tggacgttct ccagtgcgtc ggcgagctcc gcgacccgct gcgatttccc
     4201 cgcgtcggtg cgcgcgctcg gtggcatcaa cgacatcgtg acctcctggt gtccccagag
     4261 gtgtcggcag gcccgcgaga aacgttgagc gattgtcggg agaacgacgc cggagcgcgc
     4321 gcggcacggg cccgcccaac tcctggcgtc agaaggtcgt cgtgatgatg cggccgacgc
     4381 ggtcgtcagc ggaggccgag aggtcggcga cgacgaacga gcgttgcagc caccgatcgg
     4441 tgccgtcgaa gcgagcggcg aacggactgc gtccgtggac gcaccggtgg ttgtcgacga
     4501 cgaggaggtc gccggcctcg agcacgagcg acgtgtggtg cgtctcgacg agcgccgtga
     4561 gggcatcgag cgcggcctgg gcctcgtcgt cgagaccggt cgtgagctcg gcgtcgaacg
     4621 tgagggtggg gtggtcgggg tcgcccgtga gcacccgcat cggggggagg aaccgggcgt
     4681 cgacgccgcc gccgaaggac tcgtcggcgc gggtgtggaa ccggggttcg gcgagcacct
     4741 ggcgctgacg ggtgtcgagg ccggggagga tgtcgtcgat cgagcacagc gtggtcgacg
     4801 ccgccgggtc gcccttgagg cacaggagca cgaggaaccg gggcttgtgg gggtggaacg
     4861 ccgtctcggt gtggaacgcg agctgcaccc gcgacgaggt cgaggtctgg cgggcgacgt
     4921 cggaggacgt cgggacgatg ttctgcacga ggtcgccgcc gtgttcgggc aggtagccga
     4981 cgggttggcc gaggaggcgg gcaaccgtga ggagggtcag ctcgctcgtg gtgtccttgt
     5041 ccgtcggtgc ggcggggtgt gggggagtcg cggggactcg gccgacgggg agaccgcgca
     5101 ggaccacggc gccgacgccg tcgaggtcgt caccgagatc gacgagtgcg tcgtaggccg
     5161 cgtcggggag cgtgcgggcg gagcgggcgg cgaagcggac gaacgcaggg gtggtgggtc
     5221 cgtcggtggg ggcgaccggc gcagtccagg acacgggcac gaccggcacg gcccggtggg
     5281 tgatcgaggg tgtcagcgtc tgaaacatac gtgccagaat acacacgagc tgaaacaaat
     5341 gctacggcgg cacaccggcg ggcgctgggc ccggcatcag tcctgagggt cgacgagggc
     5401 gccgctgcgg cgccaggctc cctcgatgcg ttcgaggtgc gccgcggccg gggcccgcag
     5461 gcgcaccgcc acgccggcag tgatcgtcgc cagcgcggcg agcgccccga cgtagttgtc
     5521 gaacggcccg atgccgtccg cctcgatcga gatgacatgg gcggcgcccg acgccagcgg
     5581 ggagagcggc ccgtcggtga gcgcgatcag cgtcgctccg gcggccgtcg ccgcggcgac
     5641 ggcatcgagg acccagcggt cgtagcggcg caggtcgagc gcgacgacca cgtcgccgga
     5701 gtcgatgtcg gcgagctgac gggcaacgtt gaccggggat ccggtgagct cgaccaccga
     5761 cggccgcagc atcgacaact cggtcgcgaa ctggcgggcg atgcccgacg acgcgtcgcc
     5821 ggccaccacc cagaccgggc gcgaccgaat cgacaggacc ttcacgatcg cgtcgagatc
     5881 cgccgactcg acacccgcca acgtcgcctg gacgcactcg agcgcggcag cgagcccctg
     5941 tccccgcagg tcgccggggt ggggatcgtg gattcgctcg gtggcggggc gcagtcgacg
     6001 gccgagctcg gcctggacgg ccgcctgcag gtcggagaac ccgcggtacc cgagcttcgt
     6061 cgcgagtcgt acgaccgacg caccgctcga gcctgaccgg gacgcgatct cggcgaccgt
     6121 gccgaacgcg accgcctggg ggtcgtcggt gatcgacgcc gccaccttgc gctcggccgc
     6181 ggtcagtcgg tcggcggcgg cgtggatgcg gtcgaacacg tcccccatgc cgggacctca
     6241 ggggccggtc ggggcggcgg cgagcaactc gtcgcggaac gcagggtggg caatgccggc
     6301 cagcgcccgc gcccggtcgc tcgtcgacag gccccggacc tcggcggcgc cgaactcggt
     6361 gatgatcgtg tcgatctggt ggcggggcgt cgtgacgatg gtgcccggcc cgaacgtcgg
     6421 ttcgatccgc gaccgcaaca cgccgccgac ctcgacggtg gcaggaagac agatcaggga
     6481 ccggtcctcg agctcgaggc ccgagcccga cacgaagtcc tcgtgcccac cgacgccgga
     6541 gaactgcagg ccgccgatcg agtcggccgt cacctgcccc cagagatcca cggcgatggc
     6601 tccgttgatc gtcaccatcg tgtggttcgc cgcaatgcgc tcgggtgagt tgaccaccga
     6661 caccggagcg aaccgcacgt cgtcgcggcc gtcgagccag gcgtagagct cggcagtacc
     6721 ggccgcgaag gtcacgagcg agtacccctc gaactggtcc ttgtgcacgt tcgtcacctt
     6781 gcccgcctgg tggaggcgca tgaggccggt ggtgaacatc tcggagtgga cgccgtactc
     6841 gccgccgtcc ccgtgggcga gcaccgacgc gatcgtcgac gggatcgaac cgaagccggt
     6901 ctgcaacgtc gcgccgtcgg ggatgaaccg ctgcgcgtgc gccgcgatgg cgagctcgac
     6961 ctcggtgacc tccggatcga cgagctcgac gggtcggcgg tcggattcgc acaccacgtc
     7021 gacctcgtcc acgtggagcc ggtgaccctc gggagtgatg cccgccgtgc gcgggaagtg
     7081 ggggctggcc tcgacgatga gcagccggtc cgggtcggcg gcggcccggt gcagctcgcc
     7141 gacgctggcg ccggcgtgga gcgagagact catccagccg tcggcgtcgg gcgccgtggc
     7201 catcgtggcg atcacccggg ggcgccgggc ctccatgacc gtggcgaacc gacggaagtc
     7261 cgacgggacg taggcgatat tgcggcccat gtcgcggtag atgcgttcgg ccgggccgaa
     7321 gaacgtcgac gtgaggaaca ccccggggtg gtcgaacagg gcgtagaagt cgacgagcat
     7381 cgcccccagc acgtcgaagc gttgccagtc cgagcgctct cccagcgcgt gcacgaggcc
     7441 gaccggttgg ccggggccga gtggcaggcc gagggaatcg acgggatcga agagcgccgc
     7501 cgcctcggtg agcgacaggc gccgggtggg ttggcgttcg ggcacagcga gaccgtagcc
     7561 gcgcgacgga accgtgcagg cgcccggacc cgtgtcgggc ccgggcgctg ctcgaatcag
     7621 gccgctcgag tcaggactgc tcggatcagg actgctcggg gcggcggccc ttgcggcggc
     7681 tgaagcgcac gagcacgagc ccgctgccga gcgcaacgag tgcgacgagc gccatcgcgg
     7741 ccgcttcgcc gccggtcacc gggagtgagc ccgacgacgg acgcgagctc tgggtggcgg
     7801 ccttgtagac ggtcgaaccg agcacgtcgg gcatcgaggg cgtcggcggc accggggtgc
     7861 gcggcggttc ggtcacaccg atgcaggtcg tggtcgcggt gacggcgacg aggccgtccg
     7921 tgtcggtcgc cgacacgccg atgacgtcgt cggggcggag cgtcaacgtg atctgcgacg
     7981 tcgtgccgtc gacgaccttg ccgttgaccg cccacgtgaa gacgaggtcg gcgaccttgg
     8041 cgttggtcga cgtcgccacg agcttggtcg gctggccgaa cgtgaagtcg gcggggcacg
     8101 tcaccgagat cttcgggcgc tccttgccgg tgcagtcgaa cgtggccgcc gccgaggcga
     8161 gacccgtcgg atcggtggcc gtcaccgtca cgacgtcgcc cttggcgagg gtcagcgtga
     8221 ccctcgctgc ggtctgaccc gcgacgggtg tgccgttcac ggtccaggcg taggtcaggg
     8281 tgtcgccgtc gtcatccttg ccgaccgcct cgaagacgac gggctcgccc cacaccacgc
     8341 gagcggggca ggtcgtcgtc accgtcgggg tggagtcgcc gtcgcacgtg gaggtggcgg
     8401 cggtggacgt cgcacccttg gaatcggtcg cggtgaccgt cacccggtcg cccttggcga
     8461 cggtcagctc ggccgtcgcc ccggtctggc ccttcacgac gtcgtcgccg atcttccact
     8521 cgtaggtgag ctcgtcaccg tcggcgtcgg tcccggtagc ggtgaacgtg gtcggctgcc
     8581 cgtagaccag cttctcgggg cagacgaggg ccacggtcgg gcggctgttg ccggcgcagt
     8641 cggcggccgc cgacgccgac accgcgccct cggagtcggt cacggtgacg gtgagcacgt
     8701 cacccttcgc gaccgtcgcg gtgagcttgg ggctcgactc cttcggcacc ggggtgccgt
     8761 tgacatacca ggagtacgcg agcttggtgt cgccgtcggg gtcgagaccg ttggcgatga
     8821 agatcatcgg ctccccgtag acgagcttgg cggggcaggt gatcgtcacg gtcggccgcg
     8881 acgtgcccgt gcactggatc gtcgtgctgg ccgacgcggt ctcggaatcg tcgatcgccg
     8941 tgacgttgat gcggtcgccc gcctggatgg tcagcgtcgc ggtggcgccg gtctggccga
     9001 ccacggccgt gccgttgatc ttccagaggt aggtcaacgg gtcgccgtcg tcgtcgatcc
     9061 cgatcgcggt gaacgagatc ggctcgccgt acaggaccga cgtcgggcag gacagctcga
     9121 cctgcggacg gctgttgccg tcgcagttgc cggtggcggc ggccgaggac gccttgcggg
     9181 tgtcggtgac cgtcccgaga tgacatcgcc
//
LOCUS       DAOSOA010000108         9201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7470_length_9201_cov_6.893177, whole genome shotgun sequence.
ACCESSION   DAOSOA010000108 DAOSOA010000000
VERSION     DAOSOA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7470_length_9201_cov_6.893177"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PKY13_08485"
     CDS             <1..611
                     /locus_tag="PKY13_08485"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT80011.1"
                     /translation="VGGAVHDRPAPIVEAAAALAALDQFGLAPGLIADMEAACDLLDA
                     RLAELAASPSPADRLAKRIGRTMSLVYGGGAVGAAAAASWKHSINRNAKAPAFANSVP
                     GLDHDEVCGWAQHGDVTRQVFTLAVLRHGFETSDQAHRLDVTAETCDEIVAGVHPVTS
                     GATTAVGALFDLMLYGELVSLEMAARADIDPGPTPITSRYTD"
     gene            783..2213
                     /gene="ahcY"
                     /locus_tag="PKY13_08490"
     CDS             783..2213
                     /gene="ahcY"
                     /locus_tag="PKY13_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOT80012.1"
                     /translation="MDFKVADLSLAGFGRKEINLAEHEMPGLMATRAEFTDGQPLKGA
                     RIMGSLHMTIQTAVLIETLTALGADVRWVSCNIFSTQDHAAAAVVVGPEGTVDQPAGV
                     PVFAWKGETLEEYWWCTDQALRWPDGKGPNMILDDGGDATMLVHKGTEYEAHGSVPEP
                     HESNSDEWNVVLDLLAKSVASEPTRWTEVGKDILGVTEETTTGVHRLYQMFEADQLLF
                     PAINVNDSVTKSKFDNLYGCRHSLIDGINRATDVMIAGKVAVVCGYGDVGKGCAASLK
                     GQGARVIVTEIDPICALQAAMEGYQVTTLEDVLETADIFITTTGNKGIISSEHMARMK
                     HQAIVGNIGHFDNEIDMAGLGRMSDIQRVNIKPGTDEFVFPDGHSVIMLAEGRLLNLG
                     CATGHPSFVMSNSFTNQVIAQIELFTKTADYPVGVYVLPKLLDEKVARLHLDALGVKL
                     TKLSQEQADYIGVPVDGPYKPDHYRY"
     gene            2310..3437
                     /locus_tag="PKY13_08495"
     CDS             2310..3437
                     /locus_tag="PKY13_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT80013.1"
                     /translation="MKRMTAILMILLLSAVALTGCDTVRAGTRCKAGAAPGRDATHVL
                     FCQGGKWKRVMTIGQAADFILGTIPGAVTPVNATATVKAGDQLSLAVAFTVTSRTGKP
                     LPKAKVTLSAPSTGAGLVPSAELSGVTGDDGRVWFPKAVINNALGSFTLTAKVDGSDV
                     AGTVAVTVVAGEPAAITIVSGQGQTATVGTALGAPMSARVTDARGNPVSGKSVGVYYS
                     GGLQSGNPPKAVLTTDANGVVVTTLPVSAPTTPGSFPVYFEVERTSYFATFNHTVVVG
                     SPDTITPEGPSPLTAVHDQAFADPLQVLVKDRYGNAISGATVTFTAPSSGATAVLAAP
                     TAVTGTDGRASVTATAGSTVGTYQVSVAAGSANSSVSMQNT"
     gene            3669..4862
                     /locus_tag="PKY13_08500"
     CDS             3669..4862
                     /locus_tag="PKY13_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80014.1"
                     /translation="MAHRNVRRSVAVLALAASVLTVAGCDMVKQGAKCRNGAAPGRDA
                     THVLFCQGGKWKRVMTIGQAADFIMSTWPSNVELLEGGGQTTGVGEAFGQVVVRVTRK
                     DGQPVKGADVVFKGPDSGAGLAAPSGLIATDANGVARFTPVANSQLGGYAVSATVNGG
                     TSPYVVFGLNNGAAPASSIVVVSGAGQSAAAGTQFAPVGVKAVDRFGNVVQYKNFEFS
                     SPVAGVWFGPAQAFAGEDGIATTTIGTGNAAGTVPVTVKVVGTDTATTFNLTVVAAAI
                     DHTLDFFTGDNQSANPTSDPQFPRRVNNPLNVGMLDRFGNAAAGQPVIFTIQPSGGAS
                     ATFDATPNTTVTSATTATITAEADGIAKARLTGNGTPGTFTVVASFDGRTRTFTVTNP
                     ALPPA"
     gene            4911..5780
                     /locus_tag="PKY13_08505"
     CDS             4911..5780
                     /locus_tag="PKY13_08505"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT80015.1"
                     /translation="MDEFAGKVAIITGGGAGIGLAMARRFGAEGMKLVIADIQQDALD
                     DAVTALSAEGIETIGVQADVSDRAQVQAIADAAMERFGAVHLACNNAGVGSGGLSWEL
                     TEADWKWVLGVDLWSVIHGVGIFTPLIIASGGGHIVNTASMAGLTSPPFMAPYNVAKH
                     GVVALSESMFIELQMMAPNVGITVVCPGWVQTQIHNSVRNRPTTGEQAPDSAATEEDA
                     ENLRNTIGALIADGLQPSDVAEQVFTAVSTDRFYVLTHPDWSHMVLRNTERMLAGENP
                     QMAIPGQDARPGE"
     gene            5836..6477
                     /locus_tag="PKY13_08510"
     CDS             5836..6477
                     /locus_tag="PKY13_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT80016.1"
                     /translation="MGLTRCFLPVHCVVCRASGASLCPDCAALLVPPPDAGALGGLDR
                     AVALFAYDGAGRTVIRRLKFDNHRDALHPLAAMLALAVDEPIDVVTWVPTSPARRRGR
                     GYDQAELLARAVGRAAGSPVRATVRHVGGVTQTGRGRAERLTTRFEARPAARTIGANA
                     TVVVVDDVRTTGASLVGVAAALRSVGIRKVIGATLGATPRSVPFPRPDPLQSG"
     gene            6544..7200
                     /gene="raiA"
                     /locus_tag="PKY13_08515"
     CDS             6544..7200
                     /gene="raiA"
                     /locus_tag="PKY13_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT80017.1"
                     /translation="MQINVSARHTEVAKGDRDLIVEKLSRLGGKFLEMDRAEVHFFQE
                     RNPRIHDKECCEITLEGHGHHIRCKSHGPDHLTAVDRAIEKLENKLHKLKTKLSTYRT
                     HRDVTRAKQWANASTDIPDDLVREVVSTNGAAADVVEYTDASAEATELVESYRIVKTK
                     TVENMVLTPIDAAMRMDLVGHSFYFFTNVETGGPAVVYRREDGDIGLIDEGVSSDRFA
                     "
     gene            7271..>9201
                     /gene="secA"
                     /locus_tag="PKY13_08520"
     CDS             7271..>9201
                     /gene="secA"
                     /locus_tag="PKY13_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT80018.1"
                     /translation="MAFFDKLLRAGETRKVRALADLVPDIGALEPELQQLDDHALQHK
                     TVEFRERLDRGEDVDNLLIEAFAVTREAANRVIGQRHYDVQLMGGAALHFGWIAEMKT
                     GEGKTLVSSLPVYLNGLTGKGVHVITVNDYLATRDSEWMGQIHNFMGLSIGLVVPDAY
                     DWEHKRQQYAADITYGTNNEFGFDYLRDNMAMSREEQVQRGHAYAIVDEVDSILIDEA
                     RTPLIISGQLADAAKLYYRFASIVRGLKRDADYEVDEEKRTVSPLESGIEKIEKALGI
                     ENMYDDVSSNLVHQLTVALKAKELFHRDKDYLIADGEVKIVDEFTGRVLEGRRWSEGI
                     HQAVEAKEGVRIKEESQTLATITLQNYFRLYEKLAGMTGTAQTEAAELMNTYNLQVVS
                     IPTHRGIVRNDESDLIYKTEDAKFDAAVEEILSKNEHGQPVLVGTISVEKSEKLSRLL
                     DKRGIDHEVLNAKQHTREAEIVTQAGRLGKVTVATNMAGRGVDILLGGNPEGLAHKEC
                     IAEGLIDGSEEYQARFDELLPGLKAQCKAEGDKVREAGGLFVLGTERHESRRIDNQLR
                     GRSGRQGDPGESRFYLSLEDELMRIFATGAIQMVMGKNWPDDLPIEHRMVSKAIERAQ
                     NTVEQRNAEIRKNVLKYDEV"
BASE COUNT         1589 a         3181 c         3041 g         1390 t
ORIGIN      
        1 ccgtcggcgg agcggtccac gaccgaccgg cacccatcgt ggaagcggcg gcggcgctgg
       61 ccgcgctcga ccagttcggt ctggcccctg ggctcatcgc cgacatggag gccgcgtgcg
      121 atctgctcga tgcccgcctg gcggagctgg cggcgtcgcc gtcgcccgct gatcgactcg
      181 ccaagcgcat cggccgcacc atgtcgctcg tctacggcgg cggggcggtc ggcgcggcag
      241 cagcggcgtc gtggaaacac tcgatcaacc gcaatgccaa ggcgccggca ttcgcgaact
      301 cggtccccgg tctcgaccat gacgaggtct gcgggtgggc ccagcacggc gacgtcacgc
      361 ggcaggtgtt cacgctcgcg gtccttcggc acgggttcga aacctccgac caggctcacc
      421 gcctcgacgt caccgccgag acctgcgacg agatcgtcgc cggtgtgcac cccgtgacca
      481 gcggcgcgac gaccgccgtc ggggcgctgt tcgacctcat gttgtacggc gagctggtct
      541 cgctcgagat ggccgcacgc gccgacatcg accccggtcc cacgccgatc acgagccgct
      601 acaccgactg atccgccccc gctcgctgtg atggcctacg ctgtcgcgtc gtacgggctg
      661 ccgtgtaccc ggtgtctgat gaccccagcc acgcacactt cacacacgag cggtgacccg
      721 cgtgcgccga gaccgtcggc gccgagagca ccgcacacga tttgtcgcgg aggcgcgcac
      781 acatggactt caaagtcgca gacctgtcct tggccggatt cggccgcaag gaaatcaacc
      841 tcgccgagca cgagatgccc ggcctcatgg ccacccgagc cgagttcacc gacggccagc
      901 ccctgaaggg cgcccgcatc atgggctccc tccacatgac gatccagacc gcggtgctca
      961 tcgagacgct cacggccctc ggtgccgacg tgcgctgggt cagctgcaac atcttctcga
     1021 cccaggatca tgccgctgct gcggtcgtcg tcggccccga gggcaccgtc gaccagccgg
     1081 ccggtgtgcc ggtgttcgcc tggaagggtg agacgctcga ggagtactgg tggtgcaccg
     1141 accaggcgct ccgctggccc gacggcaagg gcccgaacat gatcctcgac gacggcggcg
     1201 acgccacgat gctcgtgcac aagggcaccg agtacgaagc gcacggttcg gtccccgagc
     1261 cccacgagtc caactccgac gagtggaacg tcgtgctcga cctgctggcc aagtcggtcg
     1321 cgtcggagcc gacccgctgg accgaggtcg gcaaggacat cctcggcgtg accgaggaga
     1381 ccaccaccgg tgtgcaccgc ctctaccaga tgttcgaggc cgaccagctc ctgttcccgg
     1441 cgatcaacgt caacgactcg gtgaccaagt cgaagttcga caacctctac ggctgccgtc
     1501 actccctcat cgacggcatc aaccgtgcga ccgacgtgat gatcgccggc aaggtcgcgg
     1561 tcgtgtgcgg ctacggcgac gtcggcaagg gatgtgctgc gtcgctcaag ggtcagggtg
     1621 cccgggtgat cgtcaccgag atcgacccga tctgcgcgct gcaggcggcg atggagggtt
     1681 accaggtcac caccctcgag gacgtgctcg agacggccga catcttcatc acgaccacgg
     1741 gcaacaaggg catcatcagc tccgagcaca tggcgcgcat gaagcaccag gccatcgtcg
     1801 gcaacatcgg ccacttcgac aacgagatcg acatggccgg cctgggccgc atgagcgaca
     1861 tccagcgcgt gaacatcaag ccgggcaccg acgagttcgt cttccccgac ggtcactcgg
     1921 tgatcatgct cgccgagggt cgtctcctga acctgggctg cgccaccggc cacccgagct
     1981 tcgtgatgtc gaacagcttc acgaaccagg tcatcgcaca gatcgagctc ttcacgaaga
     2041 cggccgacta tcccgtcggt gtctacgtgt tgcccaagct cctcgacgag aaggtcgccc
     2101 gcctgcacct cgacgcgttg ggtgtgaagc tcacgaagct gagccaggag caggccgact
     2161 acatcggagt gccggtcgac gggccctaca agcccgatca ctaccgctac tgatcgtccg
     2221 tttctccgcc ggttctgtgg cgtgggttcg gggtcatcgt ccccgacctg cgccatcgaa
     2281 ccggcgtgag gccgataccg ttgccgccca tgaaacgaat gacggcaatt ttgatgattc
     2341 tgctcctgtc ggccgttgcg ctgacgggct gcgatacggt ccgggccggc acacggtgca
     2401 aggccggcgc tgcgcccggt cgcgatgcca cccacgtgct gttctgccag ggcgggaagt
     2461 ggaagcgcgt gatgacgatc ggccaggccg ccgatttcat cctcggcacg atcccgggcg
     2521 ccgtgactcc ggtgaacgcc acggccacgg tcaaggccgg ggaccagctc tccctcgccg
     2581 tcgcgttcac ggtcacgagc cggacgggca agccactgcc caaggcgaag gtgacgttga
     2641 gcgcaccctc gaccggtgcg ggcctcgtgc cgtcggccga gctcagcggc gtcaccggtg
     2701 acgacggacg ggtctggttc ccgaaggccg tcatcaacaa cgcactcggc tcgttcacgc
     2761 tgaccgcgaa ggtcgacggc agtgacgtag cgggcacggt ggccgtcacg gtcgtggccg
     2821 gcgagccggc ggcgatcacg atcgtcagtg ggcaggggca gaccgcgacc gtcggcaccg
     2881 cgctgggcgc gccgatgtcc gcccgcgtga ccgacgctcg cggcaacccc gtgagcggga
     2941 agtccgtggg cgtgtactac tccggcggcc tgcaatcggg gaacccgccg aaagccgtcc
     3001 tgacgaccga cgccaacggt gtggtggtca cgaccctccc cgtgtccgcg cccaccacgc
     3061 ccggctcgtt cccggtgtac ttcgaggtcg agcgcaccag ctacttcgcg accttcaacc
     3121 acaccgtcgt cgtcggcagc ccggacacga tcacacccga gggcccgtcg ccgttgacgg
     3181 cggtgcacga tcaggcgttc gccgatccgc tccaggtgtt ggtcaaggac cggtacggca
     3241 atgcgatctc cggcgcgacc gtcacgttca cggctccgtc gagcggggca accgcagtgc
     3301 tggcggcgcc gacggcggtc accggcaccg atggtcgggc gtcggtcacc gcgacggccg
     3361 gatcgacggt cgggacctac caggtgagcg tggcggcggg ttcggcgaac tcgtccgtct
     3421 cgatgcagaa cacctgaccc accagctcca ccaccgagcg gatgccgatc ggggcgtcgg
     3481 gtatgacccg gcgccccgat gtcgcgcccg ggttcggtgc gttctctgcg tcccggccct
     3541 ccctcgcccg tctggcggtc ctgcgccgtc gacacacctc gttgtatttc atgcgtcggc
     3601 gttcgctacc atcggcggat cgcgatcgcc tgctggggga ggtcgctcca gaacgaagga
     3661 agcccgtcat ggcgcaccgg aacgtccgtc gatcagtcgc cgtgttggcg ttggccgcct
     3721 cagtgctcac cgtcgccggc tgcgacatgg tcaagcaggg tgcaaagtgc cgcaacggcg
     3781 ccgcgcccgg ccgtgacgcc acccacgtgc tgttctgcca gggcggaaag tggaagcgcg
     3841 tgatgaccat cggtcaggcc gccgatttca tcatgagcac gtggccgtcg aacgtcgagc
     3901 tgctcgaggg tggcggccag acgaccggcg tcggcgaagc gttcggtcag gtcgttgtgc
     3961 gggtgacccg caaggacggt cagccggtca agggcgccga cgtcgtgttc aagggccccg
     4021 attcgggcgc cggcctcgcc gctccgagcg ggttgatcgc taccgatgcc aacggcgtgg
     4081 cccgattcac cccggtggcc aacagccagc tcggtggcta cgccgtgagc gcgacggtca
     4141 acggcggcac cagcccgtat gtggtcttcg ggctcaacaa cggcgccgct ccggcgtcga
     4201 gcatcgtcgt cgtgtctgga gctgggcaga gcgccgcagc gggcacccag ttcgcccccg
     4261 tcggcgtgaa ggcggtcgac cggttcggca acgtcgtgca gtacaagaac ttcgagttct
     4321 cgtcgccggt ggccggcgtg tggttcggtc cggcccaggc gttcgctggc gaggacggga
     4381 tcgcgacgac cacgatcggc acaggcaacg ccgccggcac cgtgccggtg acggtcaagg
     4441 tcgtcggcac cgacaccgcc acgacgttca acctgacggt ggtggccgcg gcgatcgatc
     4501 acacgctcga cttcttcacc ggcgacaacc agagtgccaa cccgacctcg gatccgcagt
     4561 tcccgcgacg cgtcaacaac ccgctgaacg tcgggatgct cgacaggttc ggcaacgcgg
     4621 cggcgggcca gccggtgatc ttcacgatcc agccgagcgg cggggcgagc gccacgttcg
     4681 acgcgacgcc gaacacgacg gtgacgtcgg cgacgacggc gacgatcaca gcggaagccg
     4741 acggcatcgc caaggctcga ctcaccggga acggcacccc cggcacgttc accgttgttg
     4801 cgtccttcga cggtcggacg cgcaccttca cggtcacgaa cccggccctt cccccagcct
     4861 gattccgccc cgttagtctc ccgggtcgaa tccgatcgag ggggaagcac atggacgagt
     4921 tcgcgggcaa ggtcgcgatc atcaccggtg gcggggcggg catcggcctc gccatggctc
     4981 ggcgcttcgg cgccgaaggc atgaagttgg tcatcgccga catccagcag gacgcgctcg
     5041 acgacgccgt caccgcgctg agcgcggagg ggatcgagac gatcggtgtg caggccgacg
     5101 tgtcggaccg cgcccaggtg caggcgatcg ccgacgctgc catggaacgg ttcggcgcag
     5161 tccacctggc atgcaacaac gccggcgtcg gatcaggtgg cctgtcgtgg gaactcaccg
     5221 aggccgactg gaagtgggtg ctcggcgtcg acctgtggag tgtcatccac ggcgtcggca
     5281 tcttcacccc gctgatcatc gcgtcgggcg gtggtcacat cgtcaacacg gcgtccatgg
     5341 ccggcctcac gtcgccgccg ttcatggcgc cgtacaacgt ggcgaagcac ggtgtcgtcg
     5401 cgctgtcgga gtcgatgttc atcgaactgc agatgatggc gccgaacgtc ggcatcacgg
     5461 tcgtgtgccc gggatgggtg cagacgcaga tccacaactc ggtgcgcaac cgtccgacga
     5521 cgggggagca ggcgcccgac agcgctgcga ccgaggagga cgccgagaac cttcggaaca
     5581 cgattggcgc gctgatcgcc gacggtctgc agccctccga cgtggccgag caggtgttca
     5641 cggcggtctc gacagatcgc ttctacgtgt tgacccatcc ggactggtcc cacatggtgc
     5701 tccgcaacac cgagcggatg ttggccggtg agaacccgca gatggccatc cccggccagg
     5761 acgcacgacc gggggagtga cagcggtccc cacaacgggg tttcgccgtc agtggccgtc
     5821 cgtagggtcg gtcccatggg actcacccgc tgcttcttgc ccgtgcactg cgtcgtgtgc
     5881 cgggcgagcg gcgcgagtct gtgccccgac tgcgctgcgc tgctcgtgcc gccgcccgac
     5941 gccggagccc ttggaggact cgatcgcgcc gtcgccctgt tcgcctacga cggcgcgggg
     6001 cgcaccgtga tccggcgcct caagttcgac aaccatcgcg acgccctcca ccccctcgcc
     6061 gcgatgctcg cgttggcagt cgacgaaccc atcgacgtcg tcacgtgggt gcccacgagt
     6121 ccggcgaggc ggcgaggtcg cgggtacgac caggcggagc tgctcgcccg tgccgtcggc
     6181 cgggccgcgg gctcgccggt gcgtgccacc gtgcgtcacg tcggcggagt cacccaaacg
     6241 ggtcggggcc gcgctgaacg cctgaccacc cggttcgagg cccgtccggc cgcccgcacc
     6301 atcggcgcga acgccaccgt ggtcgtggtc gacgacgtcc gaaccaccgg cgcgtcgctg
     6361 gtcggcgtgg ccgcagcgct ccgctccgtc gggatccgga aggtgatcgg tgcgaccctc
     6421 ggcgcaaccc ccagatcggt gccgtttccg cgacccgatc ctctacagtc gggttgacca
     6481 acggcactcc cttcgtggac cggttgtgca gccggctgat cgagggggaa caggaggtca
     6541 accgtgcaga tcaacgtgag cgctcgccac accgaagtcg ccaagggaga tcgggatctc
     6601 atcgtcgaga agctctccag gctcggcggg aagttcctcg agatggatcg ggcggaggtg
     6661 cacttcttcc aggagcgcaa cccgcgcatc catgacaagg agtgttgtga gatcacgttg
     6721 gaaggccacg gccaccacat ccggtgcaag tcccacgggc ccgaccatct gaccgccgtc
     6781 gaccgggcga tcgagaagct cgagaacaag ctccacaagc tgaagacgaa gctgtccacc
     6841 taccgcaccc accgcgacgt gacccgggcc aagcagtggg ccaacgcctc gaccgacatc
     6901 cccgatgacc tggttcgcga ggtcgtgtcg accaacggcg ccgccgccga cgtcgtcgag
     6961 tacaccgacg ccagcgctga agcgaccgag ctggtcgaga gctaccgcat cgtgaagacg
     7021 aagacggtcg agaacatggt gctcacgccg atcgacgcag cgatgcgcat ggatctggtc
     7081 gggcattcct tctacttctt caccaacgtc gagaccggcg gccccgccgt cgtctatcgc
     7141 cgcgaggacg gcgacatcgg gctcatcgac gaaggtgtga gctccgaccg attcgcctga
     7201 accggccctc cgggcaggtc ctcctgcctg acgatgtgac ggggagtctg ccggtaggct
     7261 tggccgcgct atggccttct tcgacaaact ccttcgagcc ggcgagaccc gcaaggtccg
     7321 cgccctcgcc gatctggtcc ccgacatcgg cgcgctcgag cccgagctgc agcagctcga
     7381 cgaccacgcg ctccagcaca agaccgtcga gttccgtgag cggctcgacc gcggcgagga
     7441 cgtcgacaac ctcctgatcg aggccttcgc ggtgacccgc gaggccgcca accgggtcat
     7501 cggtcagcgc cactacgacg tgcagctcat gggcggtgcc gcgctccact tcggctggat
     7561 cgccgagatg aagaccggtg agggcaagac cctcgtgagc tccctcccgg tgtatctcaa
     7621 cgggctcacc ggcaagggcg tccacgtcat cacggtcaac gactacctgg ccacccgtga
     7681 ctcggagtgg atgggacaga tccacaattt catgggcctg tcgatcggcc tcgtggttcc
     7741 cgacgcctac gactgggagc acaagcgcca gcagtacgcg gccgacatca cctacggcac
     7801 caacaacgag ttcggtttcg actacctgcg cgacaacatg gccatgagcc gtgaggagca
     7861 ggtgcagcgc ggccacgcct acgcgatcgt cgacgaggtc gactcgatcc tcatcgacga
     7921 ggcccggacc ccgctcatca tcagcgggca gctcgcggat gccgccaagc tctactaccg
     7981 gttcgcgtcg atcgtgcgcg gtctcaagcg cgacgccgac tacgaggtcg acgaggagaa
     8041 gcgcacggtg tccccgctcg agtcgggcat cgagaagatc gagaaggcgc tcggcatcga
     8101 gaacatgtac gacgacgtgt cgtcgaacct cgtgcaccag ctcaccgtgg ccctcaaggc
     8161 caaggagctg ttccaccgcg acaaggacta cctgatcgcc gacggcgagg tgaagatcgt
     8221 cgacgagttc accggtcgtg tgctcgaggg gcgacgctgg tccgagggca tccaccaggc
     8281 ggtcgaggcc aaggaaggcg ttcgcatcaa ggaggagagc cagacgctcg ccaccatcac
     8341 gctgcagaac tacttccgcc tctacgagaa gctcgccggc atgaccggta cggcccagac
     8401 cgaggccgcc gagctgatga acacctacaa cctgcaggtg gtctcgatcc cgacgcatcg
     8461 tggcatcgtc cgcaacgacg agagcgatct catctacaag accgaggacg cgaagttcga
     8521 cgccgcggtc gaggagatcc tgtcgaagaa cgagcacggg cagccggtgc tcgtcggcac
     8581 catctcggtc gagaagtccg agaagctgtc gcgtctgctc gacaagcgtg gcatcgacca
     8641 cgaggtgctc aacgccaagc agcacacccg tgaggccgag atcgtcaccc aggccggtcg
     8701 tctcggcaag gtcaccgtcg ccaccaacat ggccggacgt ggcgtcgaca tcctcctcgg
     8761 tggcaatccc gagggcctgg cccacaagga gtgcatcgcc gaagggctca tcgacggcag
     8821 cgaggagtac caggctcgct tcgacgagct gctgccgggc ctcaaggcac agtgcaaggc
     8881 cgagggtgac aaggtgcgcg aagcgggcgg cctgttcgtg ctcggcaccg agcgacacga
     8941 gtcgcgacgt atcgacaacc agctccgcgg ccggtccggg cgccagggcg atcccggcga
     9001 gagccgcttc tacctgtcgc tcgaggacga gctcatgcgc atcttcgcga ccggcgcgat
     9061 ccagatggtg atgggcaaga actggcccga cgatctgccg atcgagcacc gcatggtctc
     9121 caaggccatc gagcgggcgc agaacaccgt cgagcagcgc aacgccgaga tccgcaagaa
     9181 cgtgctcaag tacgacgagg t
//
LOCUS       DAOSOA010000109         9197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7475_length_9197_cov_5.882301, whole genome shotgun sequence.
ACCESSION   DAOSOA010000109 DAOSOA010000000
VERSION     DAOSOA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9197
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7475_length_9197_cov_5.882301"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1068)
                     /locus_tag="PKY13_08525"
     CDS             complement(163..1068)
                     /locus_tag="PKY13_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80019.1"
                     /translation="MTSDPTTAPPSPSPSRRLWELIEPIHAVTYFSAACQDAMTEVGM
                     KGFWMGYFAARSAPFGAAAPALVEATFYNFAPTLVRRALPDAWARATPADVRTARSAA
                     AAIALRQLAPEADEYARRALPLLESAAGAARCDGRPLAAANQALAAAADPVERLWQAT
                     TTLREHRGDGHVAALVAADLSGLEAHVLLSATVVGAATPERLQQARGWTPDQWDVAVD
                     ALRSRGLLDDGGAATAEGHLLRVGVEASTDDIAWQPYRDGLTDTGLDLLRTVLRPLAT
                     VVRQSGLIPFPNPIGLPDPHGDGSR"
     gene            complement(1080..2354)
                     /locus_tag="PKY13_08530"
     CDS             complement(1080..2354)
                     /locus_tag="PKY13_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80020.1"
                     /translation="MRTKVKVGLGVAGAMVLLAVAAGVVAQHQSGTADGPPATLPPGT
                     DPMVVVRPFAPEVAWNRPVAEFGRSETYAAYAEKFWKYGSYGGWDDPDQRGDVYLELR
                     NYSTPIYDARLATSTKRAYFANFGFPPSAGYSMVIPWNDNWEAASGNDAFILMVNPDT
                     GENWVVWSYQKNNPTTCLNAANLANGFTPFRDLCTGGVGKLTNEDGSDGDYRTWNSYQ
                     NGRGMGIPKLALITTPYEVQAGSIDHALEMTVFNSMFGPPCTEAEVGTAAAGSTCGFY
                     VPPATRVEWNDGPPNNCGENTQPNTVATREKTIPEGMRFALDISDADIEAWLDARGYT
                     DPLRSTARIFAVALRDYGWIIAETGCFGLSIEIDGMVNPDAKAIWNTLGFEDTPDAGH
                     MLDGLFTEDRIYVVNPPAPFEGTTQPSPGRAR"
     gene            complement(2371..4419)
                     /locus_tag="PKY13_08535"
     CDS             complement(2371..4419)
                     /locus_tag="PKY13_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT80021.1"
                     /translation="MNRLATETSPYLRQHADNPVDWYPWGPEAFAVARETDRPVLLSV
                     GYSACHWCHVMAHESFEDPETAALMNRLFVNVKVDREERPDVDAIYMEATQAMTGSGG
                     WPMTVFMDADGAPFYCGTYFPRTGRGGMPSFTQLCAAIDDAWRHKREGLAEQAQHLTE
                     YLRRQPKFNTDAEPVGVEALGQGEEQLLRQHDTRWGGFGGAPKFPQSMSVDLLLHQAL
                     RTGSTEALAAATRSLDAMCAGGMYDHLGGGFARYSVDEQWLVPHFEKMLYDNALLLRP
                     YLHAWQMTGNAAYRRVVDETITYLLRDLRHPDGGFFSAEDADSLDAHGHSEEGAFYVW
                     SLDELHDVVAAATDDATATLFEETYGVTRSGNFETGSILFRSVGAFGDDGDHEQAGRL
                     AAARHALFEHRGRRSRPGLDDKVLTEWNGLLLASLAEAAAACGEPAWLEAAVAQAEFL
                     CRELRGADGRWHRSWQADGGARHAGYAHDYAALVDGFTRLYEATGSLRWLDEARATAT
                     ALLDLFEDTEADGLFTTGHDAEALISRPKDLMDNATPSALSTAAIGLQRLAALGSADD
                     PETARYSSSARTMMAILGHVAGETPLAFANLLLAIELDAVGITEVVIVGDRPDLVAEA
                     RATWRPLTVLAWGETGSGSLWEARENGLAYVCEGQVCHQPVGTAAELAAQLGRGSQRS
                     "
     gene            4464..5219
                     /locus_tag="PKY13_08540"
     CDS             4464..5219
                     /locus_tag="PKY13_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT80022.1"
                     /translation="MELRLDGKVALITGASKGIGKAIAAAYAAAGAQVMISSRKLEAL
                     EAARSEILAATPDADIEVFEANAGNLDAAQACVAATVERFGGIDILVNNAATNPYMGP
                     TLDIDPGRWDKTTEVNLKAPLFWSQYAWHASMKDRPGGSIINVASIGGLTVEPSLGNY
                     NVTKAALIHLTKTLAKELAPTVRVNALAPGLVKTDMARALWEGNEESVGKFVTMQRLG
                     EPDDIANAALFLASDAASWICGHTLVVDGGMLL"
     gene            5293..6918
                     /locus_tag="PKY13_08545"
     CDS             5293..6918
                     /locus_tag="PKY13_08545"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOT80023.1"
                     /translation="MDRRTFLTSLAAATVAVACTTDDDASTRSSASPPTTSPEPVTAA
                     TPAPAAALAAGSFALGVASGDPLHDRVVIWTRLASAPVAVDGGSPSTDVEVAYDVATD
                     EAFTDLVASGIATATAALAHSVHVDVTGLRPDTWYFYRFRAGDQTSPTGRTRTFPAAD
                     ASPNSFRFVFASCQDFQWGRYGAWRHAAAEADLDAVLFLGDYIYETNLGDLSPDASGD
                     RVWANGAAFTLAEYRQRYAQTKGDADLQAAHHAAPWLTIFDDHEVSNNYAGDVSQPDR
                     DRPNSRDRRLAAYQAWYEHTPIRIEPEPTAFDDLRVHRSLQFGNLASIFAIETRQHAD
                     PPPCRSDQPEDTPILALTDDGPDCAERNDPARSNLGTDQEAWLGTGLADSTTTWNIIA
                     NPLMLAGLNVGTAEAPAYTRDTWDGYTAARDRLLDGIVTADVSNPVVVTGDWHASFVL
                     DVKKTPESATIMPEFLATSMTTVNFSQDYRAANPHVRYFDGTHHGYGAVTVTPDELRC
                     EFKYVGDVWDAASPIERTDAWKVAAGSHEAEPA"
     gene            7024..8388
                     /locus_tag="PKY13_08550"
     CDS             7024..8388
                     /locus_tag="PKY13_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80024.1"
                     /translation="MRLGADPQQLDAVAAAMDRSGATLDAVARALDAAGRDLAWHGPD
                     ADRFRHRWRAELRPAVVGAAAAVRSGATDARRQAAEQRRTSGAASVAALAMVGAGSGA
                     TAGSAPSGATPSSAGVPSAPVSIRLVTAELTASGAVVEVGASVRLRVEDLGDGRSRIV
                     ELDELAAGATAQADVRVGVSFGDGRVDRGSGAAAALGASAGVERSWLVPTGDVDEFIL
                     SRLVDPNGNSGRNVVGSALAAVPGAGLAASVLGLGDGYRAVIYERPEPERVGVVAGAS
                     GSVDADARTEIPDVAVTASAATGVVLGVSRSSVDGSVSMRMSTGLVASATAGRTGSDI
                     DWTGTTTLVVDRSGTPLSLSTVATMADDRWVEQQHGVIDLTAPAVRSAARELVDAVGA
                     AATDPTQVSERVAGALRDVIVAGAVHESTRTDRYAVVDPVSYGIELPVGSAVVASERL
                     RLER"
     gene            complement(8428..>9197)
                     /locus_tag="PKY13_08555"
     CDS             complement(8428..>9197)
                     /locus_tag="PKY13_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT80025.1"
                     /translation="LACLPLHHIGGFSVITRALLCGTGLVVHPSFDAADVELAALAGA
                     THVSLVPTTLRRVDASLFRRILLGGSAIPADRPANTVATYGMTESGAGVVYDGLALNG
                     VGLMVGNDGAISLSGPSMLRCYRDGSDPKDAAGWYRTGDVGSVEPTTGRLTVAGRADD
                     LIISGGENVWPDPVERLVAADPRVAEVAVIGRPDPEWGQRVTAVVVPVDVAEPPSLEA
                     LRELVKAELPAWCAPRALELATSLPRTALGKVRRRQL"
BASE COUNT         1308 a         3256 c         3309 g         1324 t
ORIGIN      
        1 ccgcggccgc ggccgtggtc gaggtcgagg tcgaggtcga ggtcgaggcg tcgctatcct
       61 ggcgatcggg ggcgacggtg gtcgcgccgt cggtgccgct gcacgacgtc aggcccgacg
      121 acaacaggag tgcggcgatg agggaacggc ggcggccgcc gctcatcgac tcccgtcccc
      181 gtgggggtcc gggagaccga tggggttggg gaacgggatg agccccgact gtcgcaccac
      241 cgtcgcgagc gggcgcagca ccgttcggag gaggtcgaga ccggtgtcgg tgagaccgtc
      301 ccggtacggc tgccaggcga tgtcgtcggt gctcgcctcg accccgaccc gcaggaggtg
      361 cccctcagcg gtagccgccc caccatcgtc gaggaggcct cgggagcgga gggcgtcgac
      421 cgcgacgtcc cactggtcgg gcgtccaccc ccgcgcctgt tggaggcgct ccggcgtcgc
      481 ggcgccgacc accgtcgcgg acaggagtac gtgggcctcg agcccgctga ggtcggcggc
      541 gacgagcgcg gcgacgtgcc cgtcgccacg gtgctcgcgc agggtggtgg tcgcctgcca
      601 cagccgctcc accggatcgg ccgccgccgc gagggcctgg ttcgccgcag cgaggggacg
      661 gccgtcgcat cgggcggcgc ccgccgctga ttccagcagg ggcagggccc gccgggcgta
      721 ctcgtccgcc tccggagcga gttggcgcag ggcgatcgcc gcggccgccg agcgcgccgt
      781 gcgcacgtct gcgggggtgg cgcgggccca ggcatcgggc agggcgcggc ggacgagcgt
      841 gggggcgaag ttgtagaacg tcgcctccac gagggcgggc gcagcggcgc cgaagggggc
      901 actccgcgct gcgaagtagc ccatccagaa gcccttcatc ccgacctcgg tcatcgcgtc
      961 ctggcacgcg gccgagaagt aggtgacggc gtggatcggt tcgatcagct cccacaaccg
     1021 tcggctcggg gacggggagg gtggcgccgt tgtcgggtcg gaggtcatgg gggaccgttt
     1081 tacctggcgc gaccgggcga cggctgcgtc gttccctcga acggggccgg cgggttcacg
     1141 acgtagatgc ggtcctcggt gaagagcccg tcgagcatgt gcccggcatc gggggtgtcc
     1201 tcgaacccga gcgtgttcca gatcgccttg gcgtcggggt tcaccatgcc gtcgatctcg
     1261 atcgacaggc cgaagcagcc ggtctcggcg atgatccatc cgtagtcgcg gagggcgacg
     1321 gcgaagatgc gggccgtcga gcggagtggg tccgtgtacc cgcgggcatc gagccaggcc
     1381 tcgatgtcgg cgtcggagat gtcgagggcg aaccgcatcc cctccggaat cgtcttctcc
     1441 cgggtcgcga cggtgttcgg ttgagtgttc tctccgcagt tgttcggcgg gccgtcgttc
     1501 cactccaccc gcgtcgcggg cgggacgtag aagccgcacg tcgagccggc cgccgccgtc
     1561 ccgacctcgg cctcggtaca cggcggtccg aacatcgagt tgaacaccgt catctcgagg
     1621 gcgtggtcga tcgaaccggc ctgcacctcg tagggcgtgg tgatcagcgc cagtttgggg
     1681 atccccatgc cacggccgtt ctggtagctg ttccacgtgc ggtagtcgcc gtcgctgccg
     1741 tcctcgttcg tgagcttgcc cacaccaccg gtgcagaggt cgcggaacgg cgtgaacccg
     1801 ttggccaggt tggcggcgtt cagacaggtc gtcgggttgt tcttctggta cgaccacacg
     1861 acccagttct ccccggtatc ggggttcacc atcaggatga acgcgtcgtt gccgctcgcg
     1921 gcctcccagt tgtcgttcca ggggatcacc atcgagtagc ccgccgacgg cggaaacccg
     1981 aaattggcga agtaggcgcg cttggtgctc gtggcgagcc gggcgtcgta gatcggtgtc
     2041 gagtagttgc gcagctccag gtacacgtcg ccgcgctggt cggggtcgtc ccaaccgccg
     2101 tagctcccgt acttccagaa cttctcggcg tacgcggcgt aggtctcgga gcggccgaat
     2161 tcggcgaccg ggcggttcca ggcgacctcg ggagcgaagg gccgcacgac gaccatcggg
     2221 tcggtccccg gcgggagggt ggcgggcggg ccgtcggcgg tgccgctctg gtgctgagcg
     2281 accacgcccg cggcgacggc gagcagcacc atcgccccgg ccacaccgag cccgactttg
     2341 accttggtcc tcaccggccc gacgctccgg tcacgaccgc tgcgagccgc ggcccagctg
     2401 cgcagccagc tcggccgcgg tgccgaccgg ttggtggcac acctggccct cgcacacgta
     2461 ggcgagcccg ttctctcgcg cctcccacaa cgatccgctc ccggtctcgc cccacgccag
     2521 cacggtgagg ggacgccagg tcgcgcgggc ctcggccacc aggtcgggac gatcgccgac
     2581 gatgaccacc tcggtgatcc ccacggcgtc gagttcgatc gccaacagga gattggcgaa
     2641 ggccaggggc gtttccccag cgacgtggcc gaggatcgcc atcatcgttc gggccgacga
     2701 cgagtagcgg gcggtctcgg gatcgtccgc cgaacccagc gccgcgaggc gctgcaaccc
     2761 gatcgccgcc gtggacaacg ccgacggtgt tgcgttgtcc atcagatcct tggggcggga
     2821 gatgagcgcc tcggcgtcgt ggccggtggt gaacaggccg tcggcttcgg tgtcctcgaa
     2881 gaggtcgagc agcgccgtgg ccgttgcccg tgcctcgtcg agccagcgca gcgaccccgt
     2941 cgcctcgtag aggcgggtga acccgtcgac gagcgcggcg tagtcgtgcg cgtagcccgc
     3001 gtgacgggcg ccgccgtcgg cctgccagct gcggtgccag cggccgtcgg ctccgcggag
     3061 ctcgcggcag aggaactccg cctgggccac ggctgcctcg agccacgccg gttcgccgca
     3121 cgccgcggcc gcctcggcga gcgatgcgag caggagcccg ttccactcgg tgagtacctt
     3181 gtcgtcgagg ccggggcgcg accggcgacc gcggtgctcg aacagcgcgt gacgggcggc
     3241 ggcgaggcgc ccggcctgct cgtggtcgcc gtcgtcaccg aaggccccga cggaccggaa
     3301 caggatcgag ccggtctcga agttgccgct gcgggtcacc ccgtaggtct cctcgaacaa
     3361 cgtcgccgtc gcgtcgtcgg tggcggcggc cacgacgtcg tgcagctcgt cgagggacca
     3421 cacgtagaac gcgccctcct cgctgtggcc gtgggcgtcg agcgagtcgg cgtcctcggc
     3481 cgagaagaac ccgccgtcgg gatggcggag gtcgcggagc aggtacgtga tcgtctcgtc
     3541 gacgacccgg cggtacgccg cgttgccggt catctgccac gcgtgcaggt acggccgcag
     3601 cagcagggcg ttgtcataga gcatcttctc gaaatgcggc accagccact gctcgtcgac
     3661 ggagtagcgg gcgaacccgc cgcccaggtg gtcgtacatg ccgcccgcgc acatcgcgtc
     3721 gagcgagcgg gtcgcggccg ccaatgcctc ggtcgagccg gtgcggagcg cctggtgcag
     3781 caggagatcg accgacatcg attgcgggaa cttcggtgcg ccgccgaagc caccccaccg
     3841 ggtgtcgtgt tgtcgcagca gctgctcctc gccctggccg agcgcctcga cgcccaccgg
     3901 ctcggcgtcg gtgttgaact tcggctgccg gcgtaggtac tcggtgaggt gttgcgcctg
     3961 ctcggcgagg ccctcgcgct tgtgtcgcca cgcgtcgtcg atcgctgcgc acagctgggt
     4021 gaacgacggc atgccgcccc ggcccgtcct cggaaagtac gtgccgcaat agaacggggc
     4081 accgtcggcg tccatgaaga cggtcatcgg ccacccgccg ctgcccgtca tggcctgcgt
     4141 ggcctccatg tagatggcgt cgacgtcggg gcgttcctcg cggtcgacct tgacgttgac
     4201 gaagaggcgg ttcatcaacg ccgccgtctc ggggtcctcg aacgactcgt gcgccatgac
     4261 gtggcaccag tggcacgccg agtaccccac cgacaggagg acggggcggt cggtctcacg
     4321 ggcgacggcg aaggcctcgg ggccccacgg gtaccagtcg accggattgt ccgcgtgctg
     4381 gcgaagatag gggctggtct cggtggcgag gcggttcacg ggcggcacgc taccggccgc
     4441 gccgcatcga tagtctccgg ccgatggaac ttcgtctcga tggaaaagtc gcactgatca
     4501 ccggcgcgtc gaaggggatc ggcaaggcga tcgccgcggc gtacgccgct gccggcgccc
     4561 aggtcatgat ctcgtcgcgc aagctcgagg ccctcgaggc ggcgcgctcc gagatcctcg
     4621 cagccacccc ggacgccgac atcgaggtgt tcgaggccaa cgccggcaac ctcgacgccg
     4681 cgcaggcgtg cgtggcggcg accgtcgagc gcttcggcgg catcgacatc ctcgtcaaca
     4741 acgctgcgac caacccgtac atgggcccca ccctcgacat cgaccccggg cggtgggaca
     4801 agacgaccga ggtcaacctg aaggcgcccc tgttctggtc gcagtacgcc tggcacgcct
     4861 cgatgaagga ccgtcccggc gggtcgatca tcaacgtcgc gtcgatcggc ggcctcaccg
     4921 tggagccctc gctcggcaac tacaacgtca ccaaggcggc cctgatccac ctgacgaaga
     4981 cgctcgcgaa ggaactcgct cccaccgttc gggtcaacgc gctggcgccg ggcctcgtga
     5041 agaccgacat ggcccgggcc ctctgggagg gcaacgagga atcggtgggg aagttcgtca
     5101 cgatgcagcg tctcggcgaa cccgacgaca tcgccaacgc cgcgctgttc ctcgcctccg
     5161 acgccgcctc gtggatctgc ggccacaccc tcgtcgtcga cggcgggatg ctgctgtaga
     5221 cgggctctgg tcccgaaggt gaacacgagg tgaacacagc gttactctga tgcccttccc
     5281 gagggggtgg gtgtggaccg cagaacgttc ttgacctcgt tggccgcggc caccgtcgcg
     5341 gtggcgtgca ccaccgacga cgacgcctcg acgaggtcgt cggcatcgcc gccgaccacg
     5401 tcgcccgagc cggtgaccgc tgcgacgcca gcgccggctg cggcgctcgc cgccgggagc
     5461 ttcgccctcg gggtcgcgag cggtgacccg ctccacgacc gcgtggtcat ctggacccgc
     5521 ctcgcctcgg cgccggtcgc cgtcgacggt gggtcgccct ccaccgacgt cgaggtcgcc
     5581 tacgacgtgg cgaccgacga ggccttcacc gacctggtgg cctccggcat cgcgactgcg
     5641 accgcggcgc tggcccactc cgtacacgtc gacgtaaccg gcctgcggcc cgacacgtgg
     5701 tacttctacc gcttccgggc cggcgaccag acgagcccga cgggccggac ccgcaccttc
     5761 ccggcagcgg acgcgtcgcc gaactcgttc cgcttcgtgt tcgcctcgtg ccaggacttc
     5821 cagtggggtc gctacggcgc ctggcggcac gccgccgccg aggccgacct cgacgccgtc
     5881 ctgttcctcg gcgactacat ctacgagacc aacctgggtg acctctcgcc cgacgcgtcg
     5941 ggcgatcggg tgtgggccaa cggcgcggcg ttcacgctgg ccgagtaccg ccagcgctac
     6001 gcccagacca agggcgacgc cgacctccag gccgcccacc acgccgcccc gtggctgacg
     6061 atcttcgacg accacgaggt gtccaacaac tacgccggcg acgtcagcca gcccgaccgg
     6121 gaccgaccca acagccgtga ccgccggctc gccgcctacc aggcctggta cgagcacacg
     6181 ccgatccgga tcgaaccgga gccgacggcc ttcgacgacc tgcgcgtgca ccggtcgctg
     6241 cagttcggca atctcgcgtc gatcttcgcc atcgagaccc gccagcacgc cgatccgccg
     6301 ccgtgccgct ccgaccagcc cgaggacacg ccgatcctcg ccctcaccga cgacggtccc
     6361 gattgcgccg aacgcaacga cccggcccgc tcgaacctcg gcaccgacca ggaggcgtgg
     6421 ctcggcaccg gcctcgccga ctcgacgacg acgtggaaca tcatcgccaa cccgttgatg
     6481 ctcgccggcc tcaacgtcgg caccgccgag gcgccggcgt acacccgcga cacgtgggac
     6541 gggtacaccg cggcgcggga tcgcctcctc gacggcatcg tcacggccga cgtgtcgaac
     6601 cccgtggtcg tcaccggcga ctggcacgcg agcttcgtgc tcgacgtcaa gaagaccccc
     6661 gagtcggcca cgatcatgcc ggagttcctg gcgacgtcca tgacgacggt caacttctcc
     6721 caggactacc gggccgcgaa tcctcacgtc cgctacttcg acggcaccca ccacggctac
     6781 ggcgccgtca cggtcacgcc cgacgagctg cgttgcgagt tcaagtacgt gggtgacgtc
     6841 tgggacgcgg cgtcgccgat cgaacggacc gatgcctgga aggtcgccgc gggcagtcac
     6901 gaggccgaac ccgcctgaag ggttcgcccg ccgccatggg ggacgtcccc cgtggctcgt
     6961 ggcgttcgtc gccgtcatcg tggacggcat ggccgccgat gaccgagatc gccggacccg
     7021 ggcgtgaggc tcggggccga cccccaacaa ctcgacgccg tcgccgcagc gatggatcgc
     7081 agcggggcga cgctcgacgc ggtggcgcgg gcgctcgacg ccgccggtcg cgacctcgcc
     7141 tggcacggac ccgacgccga ccggttccgc catcgatggc gggcggagtt gcgaccggcc
     7201 gtcgtcgggg cagccgccgc cgtccggtcg ggtgcgaccg atgcccgtcg gcaggcggcc
     7261 gagcagcgcc gaacgtcggg cgccgcctcg gttgcagcgc tcgccatggt tggtgccgga
     7321 tcgggcgcca cggccggttc ggcaccgtcg ggcgcgacac cgtcgagcgc gggcgttccc
     7381 tcggctccgg tctcgatccg gctcgtgacg gcggagctga cggcatcggg tgccgtcgtc
     7441 gaggtcggtg cctcggtccg cctgcgggtc gaggacctcg gcgacggccg ttcccgcatc
     7501 gtcgaactcg acgagctcgc cgccggcgcc acggcccagg ccgatgtgcg cgtgggggtg
     7561 tcgttcggcg acggacgtgt cgaccgggga agcggcgcgg cggcagcgct cggtgcgtcg
     7621 gcaggcgtcg agcgctcgtg gctcgttccg accggcgatg tcgacgagtt catcctcagc
     7681 cgcctcgtcg atccgaacgg caacagcggg cgaaacgtgg tcggttcggc cctcgctgcg
     7741 gtccccgggg ccgggctggc cgcctcggtg cttggtctcg gtgacggcta ccgcgccgtc
     7801 atctacgaac gacccgagcc cgagcgagtc ggggtcgtgg cgggcgcctc gggatcggtc
     7861 gacgccgatg cccggaccga gatccccgac gtggcggtga ctgcgtcggc agcgacggga
     7921 gtggtgctgg gcgtcagtcg ttcctcggtg gacgggtcgg tctcgatgcg catgtcgacc
     7981 ggcctggtgg cctcggcgac ggcgggtcgc accggctccg acatcgactg gacgggaacg
     8041 acaacgctcg tggtcgaccg atcgggcaca ccgctgtcgt tgtcgacggt ggcgaccatg
     8101 gctgatgacc gatgggtgga gcagcagcac ggggtgatcg acctcaccgc cccggccgtg
     8161 aggtcggccg cccgagaact cgtggatgcc gtgggcgcag cggcgaccga ccccacgcag
     8221 gtgtcggagc gtgttgccgg cgccctccgc gacgtcatcg tcgccggtgc cgtgcacgag
     8281 tcgacccgca ccgatcgcta cgcggtcgtc gaccccgtct cctacggaat cgagctgccg
     8341 gtgggctcgg cggtcgtggc gagcgaacgg ctccgactcg agcgctgatc cggctcgagt
     8401 gctgatcggc ttcgagcgct gatcggccta cagctggcgg cgccggacct tgccgagcgc
     8461 ggtacggggc agcgaggtcg ccagctccaa cgcacgaggg gcgcaccacg ccggcaactc
     8521 ggccttcacg agctcgcgca gcgcttcgag cgacggcggt tcggcgacgt cgaccggcac
     8581 cacgaccgct gtgacgcgct ggccccactc ggggtcgggt cgcccgatga cggcaacctc
     8641 ggcgacgcgg gggtcggccg ccaccagtcg ctcgacggga tcgggccaca cgttctcgcc
     8701 gccgctgatg atcaggtcgt cggcgcgacc ggcgacggtg agcctgcccg tcgtcggctc
     8761 gaccgacccg acgtcacccg tccggtacca ccccgccgcg tccttggggt cggagccgtc
     8821 gcggtagcag cgcagcatcg acgggcccga cagggagatg gcgccgtcat tgccgaccat
     8881 gagccccaca ccgttgagcg ccaagccgtc gtagacgacc ccggcacccg actcggtcat
     8941 gccgtaggtg gcgacggtgt tggccggccg gtcggccgga atggccgaac cgccgagcag
     9001 gattcgtcgg aacagcgagg cgtcgacgcg gcgcagggtg gtgggcacga gcgagacgtg
     9061 ggtcgctccg gccaacgcgg cgagctcgac gtcggcggcg tcgaacgacg ggtggacgac
     9121 gaggccggtg ccgcacagca gggcccgggt gatcaccgag aagccaccga tgtggtggag
     9181 gggcaggcag gccaacc
//
LOCUS       DAOSOA010000110         8997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7708_length_8997_cov_5.284053, whole genome shotgun sequence.
ACCESSION   DAOSOA010000110 DAOSOA010000000
VERSION     DAOSOA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7708_length_8997_cov_5.284053"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PKY13_08560"
     CDS             <1..920
                     /locus_tag="PKY13_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT80026.1"
                     /translation="QAAPADHARLRKLVVPTFSPAAAERHRPQVQEVVDELVADWRPG
                     DTVDLAAELAIVAPVRIISRLLGVDPSRDDWFLTMAADIAALADPTAGPDVMDRAEVQ
                     LGHFKELLESAGSDGLYGELLGLQEDGDRLSPTETFSLVVALMTAGMETTASAMSLGA
                     LALMAHPDQADLLRSEPERWPAAVVELLRYSYIGYGLTRFTTRQVELGGHTIRKGQLI
                     WINVGAALHDPEVFPEPGRLDIGRPNAEAFPFGFGAHYCLGAALAKVEIECALRTLVD
                     RFEGMHQVGDPSWRIHMLLRGPSSLPVRL"
     gene            complement(928..1326)
                     /locus_tag="PKY13_08565"
     CDS             complement(928..1326)
                     /locus_tag="PKY13_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80027.1"
                     /translation="MTTATSTTAATRPDPMAIAVGALRLASGVSFLVAPEAANRLWGD
                     DEEPNVTTSLLLRSMGYRDALIGGVLLASALRGRPTAPWFLASAGADAADLLGGAANH
                     TRMSRQQQLKGLGGAAVGIAVGIGGALRAR"
     gene            1462..4323
                     /locus_tag="PKY13_08570"
     CDS             1462..4323
                     /locus_tag="PKY13_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT80028.1"
                     /translation="MSADRVRVARTAPAVVGSRTLRSASLDEVLPQLIDGLLDAIDSA
                     VIVVDPDGVVELWNDAAERLYGWSAAEAVGTSLADLIVPISQSDVGHEIAASLRNGDG
                     WKGTFLCRRRDGSHVPVRVTDVPVPGRDGSVRAVIGISRNVTDLLDADRDGRTIAALS
                     HASRDAVMTAVDGRVATWSAGAQRLFGYSEAEIIGAVAATFLPDETREAAAAVQARVE
                     AGETIIGFETDCVARDGGRVPVSIDVAPVFDAAAQRWVAVVTVRDRRTRDEVVEAAIV
                     AESRSRALLSQASETVFILEADYTLRSASAAVERVLGCAPEDLIGVEASRFMHPDDLP
                     KVRDAFDLAVSAPVGRAQVTYRRRNAAGDWAWAEATITNQLDDPVVAGVVANVRDVDE
                     RERALASVVEREARLSAVMSRSSDVAIFFDRDGTIRWVSPAITAALGLAPDNLLGTNA
                     IDLVHPEDRGVALRTFGRWLEAPSEHVRVEFRFLDPAGRVRWVEQVATDLTDDPSVGC
                     IVANLRDVTDRRLAHDELARLALVDTLTGLPNRNALQELAIAALDRSPDDRFCAIAFF
                     DLDDFGDVNDALGHVAGDELLVGIARRVEAALPANATLTRFGGDQFAVLCDGVAHLPE
                     TLAITAAVQQALAAPFVIDQKEVFAVVSFGLALSPPDDIDVLLRRADVALRRAKSAGR
                     GQTVVFEREQGTESVHRLHYAGEVRRAIARGEIVAHYQPVVDLATGRAVGVEALARWD
                     HPDRGRIPPDLFIPVAESTGLIGELGDRILEQACADAMRWLERGRRLQLAVNASAVQL
                     MDPAFPDRVAATLRNQGMSPQQLAIEVTETAALRDLGLASQTLDALRCQGIELSLDDF
                     GTGYSSLSLLKRLPVGALKVDRSFVSGLGQSPEDDQIVTGVIGLALALGFIVVAEGVE
                     TVEQADALRRLGCHYGQGYLWAPAVPADDLLATIASFEG"
     gene            complement(4320..4838)
                     /locus_tag="PKY13_08575"
     CDS             complement(4320..4838)
                     /locus_tag="PKY13_08575"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT80029.1"
                     /translation="MLRDATLDDLPLLGPIEAAGDARFAAAGHPELADGSTIPLDVAR
                     RSIAEERLIVADVDATVVAWAQFSRDPGEWCLGQISVLPEHQGAGIGTALLGEVIARA
                     VTAGVRTLVLNTQDDVPWNRPWYEHAGFVVVPEAQWTATMRDDTAAQVAAGLDWSTRV
                     HMRLTLAGTDRS"
     gene            4888..6345
                     /locus_tag="PKY13_08580"
     CDS             4888..6345
                     /locus_tag="PKY13_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT80030.1"
                     /translation="MVWVRRVLSFLGVAAAVLIVAPMIGSSTTEVGPGEVTIGFHPAS
                     SGSTTLAVPPIGKAGAPTHRGPVEVRLELKSLDVARLVGTDGRVDAGALKASIDDDLR
                     GAAAGIVLRFCAVGLVVGAVAAGLLPNRRLLSVALGAVEGLVVSALLVASAVPGFDVG
                     RFQDLTFEGPITTSGQIVSALSATGGPVGRRVDVLAEKIADLYSASATDDIGNDEGEV
                     VVLHISDLHLNPLGAQLARRLAVAFEVDAVVDTGDTTSFGTDFEGAYAELLADFPVPY
                     LFVAGNHDSRPNRAAIKATPGVIPLHNTSYDVGEGDDRVRIAGFDDPLITTTEDVPRA
                     EREQVELDATPRLNALIEREHPDVLAIHNPVILQKVVGMVPLALGGHQHQAKLGARSG
                     TLISLAGSTGATGLGSLLVDADLPASASLLRFRGGVLVSIDDLEAVGTSGDLTIRRHT
                     ITAADREADNADFIGRDADEGRLPAADETTTTTSG"
     gene            6469..6663
                     /locus_tag="PKY13_08585"
     CDS             6469..6663
                     /locus_tag="PKY13_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOT80031.1"
                     /translation="MRLVIDNERCEGHGRCYSIAKDLFEPDDIGNGVVIGDGVVPPDR
                     LHDAELAVENCPELAIRLEP"
     gene            6663..7880
                     /locus_tag="PKY13_08590"
     CDS             6663..7880
                     /locus_tag="PKY13_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT80032.1"
                     /translation="MGRHEPVVDWATDFDHTDDAYVADPFGIWDELRTTCPVAHTERY
                     NGAWLPTRHEDIAAIAYDTDRFTSRSVIMSEFRAPLELAPQGIAPPISSDPPFHKGAR
                     RVLLPAFAPQSVDRLEASTRAYCEELVASLRADLDGGVDVVDAAVGYSQHIPVRVIAT
                     MLGLPESDGDRFRGFVNHVLEGVDKPMEERALGMGELFEYLQAQIHDHLDRPRDDLIS
                     YLLESEMDGQPLDVFHVARTVALLLIAGIDTTWSAIGASLWHLAGHPADRERLVAEPE
                     LLPTAIEEFLRAYAPVTMARLVREDMDFAGCPMKANDFVLLPFPAANRDPEAFDRADE
                     VLIDREVNRHAAFGLGIHRCAGSHLARMELRVALEVWLEAFPSFELADPSAVRWSGGQ
                     VRGPRTLPVRVMR"
     gene            complement(7902..8708)
                     /locus_tag="PKY13_08595"
     CDS             complement(7902..8708)
                     /locus_tag="PKY13_08595"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOT80033.1"
                     /translation="MNHLFRDLAPVTDSAWAQIEDEATRSLRHFLAGRKLIDMTGPHG
                     WDHSAVDLHRIAALESPIQGTEAAVRRVLPLIEVRIPFALKLSELAAADRGADDLDLD
                     AVVEAARLAAVAEDGAIFHGFEPAGIEGLGGASPHDPVDISDDYDEYPSHVAKAVAVL
                     RAAGVDGPYAIALGPRCYTGVVESTEHGGYPVFEHIKMILGGPIIWAPAVNGAVVLSQ
                     RGGDAEVVIGQDFAIGYRGTTGDEVNLYLEESFTFKISSPEAAVALTYPA"
     gene            complement(8705..>8997)
                     /locus_tag="PKY13_08600"
     CDS             complement(8705..>8997)
                     /locus_tag="PKY13_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOT80034.1"
                     /translation="YDQRAAVATDPDLAAVLGHNRDEEKEHFVMTLEWLRRNDPKLDE
                     HLRTYLFTDPQDIIEVEKSAEHGGDEGSSGAESTATNSLRVGNLRNETEGTL"
BASE COUNT         1221 a         3200 c         3196 g         1380 t
ORIGIN      
        1 tccaggcggc ccccgccgac cacgcccgcc tccgcaaact cgtcgtgccc acgttctcgc
       61 cggccgccgc cgaacggcat cggccccagg tccaagaggt cgtcgacgag ttggtggcgg
      121 actggcggcc cggcgacacc gtcgacctgg ccgccgagct ggcgatcgtc gccccggtgc
      181 gcatcatcag ccgactcctc ggtgtcgacc cgagccgaga cgactggttc ctgacgatgg
      241 ccgcggacat cgccgccctg gccgatccca ccgccggtcc cgacgtcatg gatcgtgccg
      301 aggtccagct cgggcacttc aaggagttgt tggagtcggc gggctccgac gggctctacg
      361 gcgagctgct cggcctccag gaggacggcg accggctcag cccgaccgag acgttctcgc
      421 tcgtcgtggc gctcatgacc gccggtatgg agaccaccgc gagcgcgatg tcgctcggcg
      481 cgctggccct catggcgcac cccgaccagg ccgacctgct ccgctccgag cccgagcgct
      541 ggcccgccgc cgtcgtcgag ctgctgcggt acagctacat cgggtacggc ctcacacgct
      601 tcacgacgcg gcaggtcgag ctgggcggtc acacgatccg caaggggcag ctgatctgga
      661 tcaacgtcgg tgccgccttg cacgaccccg aggtgttccc cgagcccggg cgactcgaca
      721 tcggtcggcc caacgccgag gcgtttccct tcgggttcgg ggcgcactac tgcctgggcg
      781 ccgccctcgc gaaggtcgag atcgagtgcg cgctgcgaac cctggtcgac cggttcgagg
      841 ggatgcacca ggtcggtgac ccgtcgtggc gaatccacat gctgctgcgc ggtccgtcgt
      901 cgctgccggt ccgtctctga cgtcgggtca ccgggcccgg agcgccccgc cgatcccgac
      961 ggcgatgccg acggcggcac cgcccagccc cttgagctgc tgctgacgac tcatccgtgt
     1021 gtggttcgca gcgccgccca gcagatcggc ggcgtcggcc ccggccgagg cgaggaacca
     1081 cggcgcggtc ggtcggcccc gcagcgcgct ggccaggagg accccgccga tcagggcgtc
     1141 gcggtacccc atcgatcgga ggaggaggct ggtcgtcacg ttgggctcct cgtcgtcgcc
     1201 ccacaggcgg ttcgccgcct cgggggcgac gaggaacgac acccccgacg ccagtcggag
     1261 ggcaccgacg gcgatggcca tcgggtcggg acgggtcgcg gcggtcgtgc tcgtggcggt
     1321 ggtcatgccg cagtctggcc cgggcggcgt cgtccgcgtc ggccggatgc tcgggcggcg
     1381 gcgtcaggct cccctgggcc ttttcgccct tggttgggtg aaatgactca tcggcgcggt
     1441 gatgtggccg atggctgtgc catgagcgca gatcgtgttc gggtcgcacg gaccgcgccc
     1501 gccgtcgtcg ggtcgcgcac gctgcgctcg gcatcgctcg acgaggtgct cccacagctg
     1561 atcgacggtc tgctggacgc catcgactcc gccgtgatcg tcgtcgaccc cgacggcgtc
     1621 gtcgagctgt ggaacgatgc cgccgaacgg ctgtacggct ggtcggcggc cgaggcggtc
     1681 ggcacgtcgc tcgccgacct catcgtcccg atctcgcagt ccgacgtggg acacgagatc
     1741 gccgcgtcgc tccgcaacgg tgacgggtgg aagggcacat tcctctgccg tcgccgggac
     1801 gggtcccacg tccccgtgcg ggtcaccgac gtccccgtcc cgggacgcga cggctcggtc
     1861 cgtgcggtca tcggcatctc ccgcaacgtg accgacctgc tcgacgccga ccgcgacggc
     1921 cggaccatcg cagccctgtc gcatgcatcg cgcgatgcgg tcatgaccgc cgtggacgga
     1981 cgggtcgcga cgtggagcgc tggcgcccag cgcctgttcg ggtactccga ggccgagatc
     2041 atcggtgccg tcgctgcgac gttcttgccc gacgagaccc gggaggcggc agccgcggtg
     2101 caggcccggg tggaggcggg ggagacgatc atcgggttcg agaccgactg cgtcgcccgc
     2161 gacggcggac gggtcccggt gagcatcgac gtcgcgccgg tcttcgacgc cgcggcccag
     2221 cgctgggtcg cggtcgtgac ggttcgcgac cggcgtaccc gcgacgaagt ggtcgaggcg
     2281 gcgatcgtcg ccgaatcccg ctcgagggcg ctgctctccc aggcctccga gaccgtgttc
     2341 atcctcgagg ccgactacac ccttcggtcc gccagcgctg cggtcgagcg ggtgctcggg
     2401 tgcgcgccgg aggacctcat cggtgtcgag gcgagccggt tcatgcaccc cgacgacctc
     2461 ccgaaggtcc gcgacgcgtt cgacctcgcg gtgtctgcgc cggtcggtcg cgcccaggtc
     2521 acctaccgcc gccgcaacgc cgccggcgac tgggcctggg ccgaggcgac gatcaccaat
     2581 cagctcgacg atccggtcgt ggccggcgtc gtcgccaatg ttcgcgacgt cgacgagcgg
     2641 gaacgggcgc tggcctcggt cgtcgagcgc gaggctcggc tctcggccgt gatgagccgc
     2701 tcgagcgacg tcgcgatctt cttcgatcgg gacgggacca tccgttgggt cagccccgcc
     2761 atcacggccg cgctcgggct ggcccccgac aacctgctcg gcaccaacgc catcgacctg
     2821 gtgcaccccg aggaccgcgg cgtcgcgttg cgcaccttcg gccgctggtt ggaggcgccg
     2881 tcggagcacg tgcgcgtcga gttccggttc ctcgacccgg caggtcgggt ccgttgggtc
     2941 gaacaggtgg cgaccgacct gaccgatgac ccctccgtgg ggtgcatcgt cgccaacctg
     3001 cgtgacgtca ccgatcggcg tctcgcccac gacgaactcg cccggctcgc gctcgtcgac
     3061 acgctcacgg gcctcccgaa ccgcaacgcg ctccaggagc tggcgatcgc ggcactcgac
     3121 cgatcgcccg acgaccggtt ctgcgccatc gccttcttcg acctcgacga cttcggcgac
     3181 gtcaacgatg cgctgggcca cgtcgccggc gacgagctgc tcgtcggcat cgcccgacgg
     3241 gtcgaggcgg cgcttcccgc gaacgcgacg ttgacgcggt tcggcggtga ccagttcgcc
     3301 gtgctgtgcg acggcgtcgc gcatctgccc gagaccctcg cgatcacggc tgcggtgcag
     3361 caggccctcg cggctccgtt cgtcatcgac cagaaggagg tgttcgccgt cgtgagcttc
     3421 ggtctggcgc tgagccctcc cgacgacatc gacgtgctcc tgcgtcgcgc cgacgtggcg
     3481 ctccggcggg cgaagtcggc cgggcggggc cagaccgtcg tcttcgaacg cgagcagggc
     3541 accgagagcg tgcaccgact ccactacgcc ggcgaggtgc gacgtgcgat cgcccggggc
     3601 gagatcgtgg cccactacca gcccgtcgtg gacctggcca cgggccgcgc cgtcggtgtc
     3661 gaggcgctcg cccgatggga ccatcccgac cgcggtcgga tcccacccga cctgttcatc
     3721 cccgtcgcgg agtcgaccgg tctgatcggc gagctcggcg accggatcct cgaacaggcg
     3781 tgtgccgacg caatgcggtg gctggagcgg ggccgccggc tgcagctcgc ggtgaacgcc
     3841 tcggccgtgc agctgatgga tccggcgttc cccgaccggg tcgcggcgac gttgcgcaac
     3901 cagggcatga gcccccagca gctcgccatc gaggtcaccg agacggcggc gctgcgcgac
     3961 ctcggcctcg cgtcacaaac gctcgacgcc ctgcggtgcc aggggatcga gttgtccctc
     4021 gacgatttcg gcacgggcta ctcctccctg tcgctgctca agcggcttcc cgtcggagcg
     4081 ctcaaggtcg accgcagttt cgtgagtggc ctcggccagt cgcccgagga cgaccagatc
     4141 gtgaccggtg tgatcggcct ggccttggcg ctcgggttca tcgtcgtggc cgagggggtc
     4201 gagaccgtcg aacaggccga cgcactgcga cggctcggct gtcactacgg gcaggggtac
     4261 ctgtgggcgc cagcggtgcc cgccgacgac ctgctcgcca cgatcgcctc cttcgagggc
     4321 taagaccggt cggtccccgc gagcgtcagg cgcatgtgca cccgggtcga ccagtcgagg
     4381 ccggcggcga cctgcgctgc ggtgtcgtcg cgcatcgtgg cggtccactg cgcctcgggg
     4441 acgacgacga aaccggcgtg ctcgtaccac ggccggttcc acggcacgtc gtcctgggtg
     4501 ttgagcacga gggtccgcac tcccgcggtg acggctcgtg cgatcacctc acccagcagc
     4561 gccgtgccga tgccggcgcc ctggtgctcg ggcaggacgg agatctgacc gaggcaccat
     4621 tcgccggggt cacggctgaa ctgcgcccac gcgacgaccg ttgcgtcgac gtcggcgacg
     4681 atgaggcgct cctcggcgat gctgcgccgg gcgacgtcga gggggatcgt gctcccgtcg
     4741 gccagctcgg gatggcccgc cgctgcgaag cgggcatcac ccgcggcctc gatcgggccc
     4801 aagaggggaa gatcatcgag cgtggcgtcg cggagcaccg ggacaggcta cgcccggccg
     4861 gcgcgggcca gccggtagcg tggcgccgtg gtctgggtcc gtcgtgtgct gtcgttcctg
     4921 ggcgtcgctg cggcggtcct catcgtcgca ccgatgatcg gatcgtcgac gaccgaggtc
     4981 ggtcccggcg aggtcaccat cgggttccac ccggcgtcga gcggcagcac cacgttggcc
     5041 gtgccgccga tcggcaaggc cggtgccccg acccatcggg gccccgtcga ggttcgtctc
     5101 gagctgaagt cgctcgacgt cgcccgcttg gtcggaaccg acgggcgcgt cgacgccggc
     5161 gcgctcaagg cgtcgatcga cgacgatctc cgcggtgcgg cggcggggat cgtgctgcga
     5221 ttctgcgccg tcggcctcgt ggtcggagcg gtcgcggcgg ggctgctccc gaaccgccgc
     5281 ttgctgagcg tggccctcgg cgccgtcgag gggctcgtgg tctcggcgct gttggtcgcc
     5341 tcggccgtgc ccgggttcga cgtggggcgg ttccaggacc tcacgttcga gggcccgatc
     5401 accaccagcg gacagatcgt gtcggcgctc tccgcgaccg ggggaccggt cgggcgccgc
     5461 gtcgacgtcc tggcggagaa gatcgcagac ctctactcgg cctcggcgac cgacgacatc
     5521 ggcaacgacg agggtgaggt cgtggtgctc cacatctccg acctccacct caacccgctc
     5581 ggcgcccagc tcgcccggcg gctcgcagtg gcgttcgagg tcgacgccgt cgtcgacacc
     5641 ggcgacacca cgtcgttcgg caccgacttc gagggggcgt acgccgagct gctggccgac
     5701 ttcccggttc catacctctt cgtcgccggc aaccacgact cgcgcccgaa ccgggccgcg
     5761 atcaaggcga caccgggggt gatcccactc cacaacacgt cctacgacgt cggtgagggc
     5821 gacgaccggg tgcggatcgc cggcttcgac gacccgctca tcaccaccac cgaggacgtt
     5881 ccccgtgccg aacgtgagca ggtcgagctc gacgccactc ctcggctcaa cgcgctgatc
     5941 gaacgtgagc atcccgacgt gctggcgatc cacaatccgg tgatcctcca gaaggtcgtc
     6001 ggcatggtgc cgctcgccct cggtggacac cagcaccagg ccaagctcgg ggcgcgctcg
     6061 ggcaccctca tctcgctcgc cgggtcgacg ggagcgaccg gcctcgggtc gctgctggtc
     6121 gacgccgacc tgcccgcgtc cgcgtcgctc ctccggttcc gcggcggggt gctggtgtcg
     6181 atcgacgatc tcgaggccgt cggcacgtcg ggcgacctca ccatccgccg ccacaccatc
     6241 acggcggccg accgggaggc cgacaacgcc gacttcatcg gccgcgacgc cgacgaggga
     6301 cgactgccgg cggccgacga gacgaccacc acgacgagcg gctgagctga cacggcggcc
     6361 gttctggagc gctgaggtga cagaaactgt cacctcagcg ctccagcgcg aggggagggc
     6421 aactcagcgc tccggtcctg ggaggcggcg cggcagtagc gtgcgctcat gcgactcgtg
     6481 atcgacaacg aacggtgcga gggccacggc cgctgttaca gcatcgccaa ggacctcttc
     6541 gaacccgacg acatcggcaa cggtgtggtc atcggcgatg gggtggttcc acccgaccga
     6601 ctccacgacg ccgagctggc ggtcgagaac tgccctgagc tggccatccg actggagccc
     6661 tgatgggccg ccacgagccg gtcgtcgact gggcgaccga cttcgaccac accgacgacg
     6721 cctacgtcgc cgatcccttc ggcatctggg acgagctgcg tacgacgtgc cccgtcgccc
     6781 acaccgagcg gtacaacggc gcgtggttgc cgacccgcca cgaggacatc gcggccatcg
     6841 cgtacgacac cgaccggttc acgtcccgca gcgtcatcat gagcgagttc cgggcaccac
     6901 tcgaactcgc gccccaggga atcgccccgc cgatctcgtc cgacccgccg ttccacaagg
     6961 gggcgcgtcg agtcctcctg ccggcgttcg ccccgcagtc cgtcgaccgt ctcgaagcgt
     7021 cgacccgtgc ctactgcgag gagttggtgg cctcgctgcg ggccgacctc gacggcggcg
     7081 tcgacgtggt ggacgccgcg gtcggctatt cgcaacacat cccggtccgg gtgatcgcca
     7141 cgatgctcgg cctgcccgag tccgacggcg accgcttccg tgggttcgtg aaccacgtgc
     7201 tcgaaggggt cgacaagccg atggaggagc gcgccctcgg catgggcgag ctgttcgagt
     7261 acctccaggc gcagatccac gaccacctcg accggccccg tgatgatctg atcagctacc
     7321 tgctcgagtc ggagatggac ggccagccgc tcgacgtgtt ccacgttgcc cgcaccgtcg
     7381 cgctgctcct catcgccggc atcgacacga cgtggagcgc gatcggcgcg tcgctgtggc
     7441 acctcgccgg gcaccccgcc gaccgcgagc gcctcgttgc ggagcccgag ctgctgccga
     7501 cggccatcga ggaattcctg cgggcctacg caccggtgac catggcccgg ctggtccgtg
     7561 aggacatgga cttcgccggt tgcccgatga aggccaacga cttcgtgctc cttccgttcc
     7621 cggctgcgaa ccgtgacccc gaggcgttcg atcgggccga cgaggtgctc atcgaccgtg
     7681 aggtcaaccg tcacgccgcg ttcggtctcg gtatccaccg gtgcgcgggc tcgcacctgg
     7741 cacggatgga gcttcgtgtc gcactcgagg tgtggctcga ggcgttcccg tcgttcgagc
     7801 tggccgaccc gtcggccgtg cggtggtccg gtggccaggt ccgtggcccg cggaccctgc
     7861 ccgtccgggt gatgcgctga tgtcgacccg gccgagcggg actacgcggg gtaggtcagg
     7921 gccacggcgg cctcgggtga gctgatcttg aaggtgaagc tctcctcgag atacaggttc
     7981 acctcgtcgc cggtggtgcc gcggtagccg atcgcgaagt cctggccgat caccacctcg
     8041 gcgtcgccgc cgcgctggct gagcacgacc gcgccgttga cagcgggcgc ccagatgatc
     8101 gggccgccga ggatcatctt gatgtgctcg aacacggggt agccaccgtg ctcggtcgac
     8161 tcgacgacgc cggtgtagca gcgggggccg agggcgatcg cgtagggacc gtcgacgccg
     8221 gcggcccgca gcacggcaac ggccttggcg acatggctcg ggtactcgtc gtagtcgtcg
     8281 gagatgtcga cggggtcgtg ggggctggcg ccaccgaggc cctcgatgcc cgccggctcg
     8341 aacccgtgga agatcgcgcc gtcctcggcg accgcggcga ggcgggcggc ctcgacgacg
     8401 gcgtcgagat cgagatcgtc ggctccgcgg tcggcggcgg cgagctcgga gagcttgagc
     8461 gcgaacggga tgcgaacctc gatgagcggg aggacccggc gcaccgcagc ttcggttccc
     8521 tggatggggg actcgagggc ggcgatccgg tggagatcga ccgcgctgtg gtcccagccg
     8581 tggggaccgg tcatgtcgat gagcttgcgg ccggccagga agtggcgcag cgagcgggtc
     8641 gcctcgtcct cgatctgggc ccaggcggag tcggtgacgg gtgcgaggtc ccggaagagg
     8701 tggttcacag tgttccctcg gtctcgttgc ggaggttgcc cacccgcagg ctgttggtgg
     8761 cggtggactc ggcgccggac gagccctcgt cgccgccgtg ctcggcggac ttctcgacct
     8821 cgatgatgtc ctgggggtcg gtgaagaggt aggtgcggag gtgttcgtcg agcttggggt
     8881 cgttgcgccg caaccactcc agcgtcatca cgaagtgctc cttctcctcg tcgcggttgt
     8941 ggccgaggac ggccgccagg tcgggatcgg tggcgactgc ggcccgctgg tcgtacc
//
LOCUS       DAOSOA010000111         8987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7722_length_8987_cov_6.195477, whole genome shotgun sequence.
ACCESSION   DAOSOA010000111 DAOSOA010000000
VERSION     DAOSOA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7722_length_8987_cov_6.195477"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKY13_08605"
     CDS             complement(<1..373)
                     /locus_tag="PKY13_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80035.1"
                     /translation="MVTNTSSHPGEGPKSQLASDIAAIGVALRAVVSELRPNDVPLPF
                     APEVLAAFADVQRVAESGRVLMTARAAQAREWERQGYASAADWLAAQQGTTTGRARAD
                     LETSGHLDNLEATADAVRAGHL"
     gene            511..2661
                     /gene="glgX"
                     /locus_tag="PKY13_08610"
     CDS             511..2661
                     /gene="glgX"
                     /locus_tag="PKY13_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOT80036.1"
                     /translation="MTTTDPPATPPVEIWPGRPAPLGAHWDGQGTNFAVFSEQAESID
                     LCLFDLDGEAAGTETCIPIEEVDSHTWHVYLPGVGPGTHYGYRVTGPWNPDGGQWFNH
                     NKLLLDPYAKAIEGEVAWDEACFAYVRGDEDTMNETDSAPFVPRSVIATPWFDWSTDR
                     PPDTPMERSVIYELHVKGFTQLHPAVPEHLRGTYAGLAHPEVIGYLTDLGVTAVELQP
                     VHHFIHDSHLVEQGLRNYWGYNSIGFFAPHAGYSSATRAEDAAGEFKSMVRDFHQAGL
                     EVILDVVFNHTAEGNHLGPVLSFKGLDNHAYYRLMDDEPKYYRDFTGTGNSLNMRHPS
                     SLQLVMDSLRYWVTEMHVDGFRFDLAAALARELYDVDRLSAFFDLIQQDPIISRVKLI
                     AEPWDVGEGGYQVGNFPPLWSEWNGKYRDTIRDLWRGEGGVLGDVATRMAGSSDLYSW
                     ERGSPTASINFVTAHDGFTLRDLVSYNEKHNEANGEDNNDGESHNRSWNCGTEGPTDD
                     PDILRLRGQQLRNLLATLLMSQGVPMLLAGDELGRTQQGNNNTYCQDNELNWIDWTLL
                     DEDADGGGPDAGSGKGLWEATKQLIALRAEHPALRRRRWLTGHEDGEAAVRWLHPDGH
                     DMADGDWDGSSEMAMLLDGTVIPERGPRGEVIRDDRILLLIDAGSEPTDWSLPPTPGS
                     WVEQFSSALGSQLNDDGTYRTPGRSVVVFVEAAD"
     gene            2662..4092
                     /locus_tag="PKY13_08615"
     CDS             2662..4092
                     /locus_tag="PKY13_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT80037.1"
                     /translation="MPAPDVRWLPEGIGGPALEDNPAAFEWTDDGFRPEPWPHAVLYE
                     LHLGTFSPSGTAVAAIDHLDHLVELGVTHVEVMPLGTYGGRWGWGYDGVYWSAPQHTY
                     GSPNDLRAFVDACHARGLGVIVDVVYNHLGPVGADDPGFEPFLTDAHHTPWGKAINLD
                     GPGSRVVRDRIVDDATMWIVDYHADGLRLDAIHALVDDSPEHLLAELSRTVEGLGLDR
                     EVVLIDEDERPDSTPVRPRTDGGYGLTAKWADELHHAVHAYLTGERHAYYEPYGDPEL
                     IGKELASGAPWKVVSLQNHDQVGNRPFADRLHQTTSIETVLTVLPLFLLGPSVPMLFQ
                     GEEWAASTPFPYFADHHGELAGAIREGRAREFAWVAAEDGPPSFPNGLPDATAESTYR
                     SALLRWGEIAAPEHAAVLDRYRELIALRRSLPHVFGADAPCVIDQEGAVITMRRGGSA
                     GDRGEGPGVATVVCDLDAPRVTITGV"
     gene            4164..4460
                     /locus_tag="PKY13_08620"
     CDS             4164..4460
                     /locus_tag="PKY13_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOT80038.1"
                     /translation="MIRRTKSRTPNSVKVSFALPLDVAPNPVSVVGDFNDWDPTAHPL
                     RKRSNGTRSVTVELAEGESVQFKYLGDNGDWFPEHEADGAVVNEWGEVNSRLDL"
     gene            4457..6439
                     /locus_tag="PKY13_08625"
     CDS             4457..6439
                     /locus_tag="PKY13_08625"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HOT80039.1"
                     /translation="MSGRHSSTKVRDGGVADPSSEPAPAPTRLAIEVIHPIVDAARFT
                     AKASVIEPVELVADVFADGHDHLAASATFRHTGFPVETDIDLVPVGNDRWTGSVALAR
                     IGRWEFTVSAWIDPWEYWRRSIAARQAAGADVSAELLDGAALLRRAADAGFDVPLDVD
                     PAATSADPDVATTMRSWARTLPGGTTTPPLPLEVARERAAYGAWYELFPRSTVVGGPE
                     HEHGTLVDLVDRLGHVADLGFDVVYLPPIHPIGLTARKGPDNRLTADPADPGSPWAIG
                     AADGGHTAVHRELGTIADFDRVVAAANERGLEIALDLAFQCSPEHPWVTEHPQWFARR
                     ADGTIRTAENPPKRYEDIYPIDFSCADWESLWTALADVVRHWVDHGVKIFRVDNPHTK
                     PFAFWQWLIGEIRADHPDVVFLAEAFTRPRVLERLAKVGFDQSYGYFTWKRTPTELRE
                     YLRDLDEVADFLRPNLWPNTPDILTEQLQNGGRPVFESRAVLAALLAANYGVYGPAYE
                     LLEAQPVRRPSEDYASSEKYEVRRWDLDEPQSIAPLLRRLNEIRAAHPALHSDRRRVF
                     HHCDDPGVLAWSKRSFDGRDVILVIVNVEADLTRSGMVRLDLDALGLPPGATFTVADL
                     LSGEIYQWQGADNYVALDPHRRVAHVFEVTASEGTS"
     gene            6436..>8987
                     /gene="treS"
                     /locus_tag="PKY13_08630"
     CDS             6436..>8987
                     /gene="treS"
                     /locus_tag="PKY13_08630"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HOT80040.1"
                     /translation="MTAVLRPPTPPSPKVPRPAPVPALVGTADDPEWYRSGVIYEMHV
                     RAFADSNGDGIGDFPGLTAHLDYVAELGVTAIWLLPFYPSPGRDDGYDIADYMSVNPD
                     YGTLAQFKKFLKAAHDRGIRVITELVVNHTSDQHPWFQRARRAPAGSRWRNFYTWSDD
                     DSRWPDARIIFSDTEASNWAWDPVAGQYYWHRFFSHQPDLNYDNPEVQTAITKVVDFW
                     LGMGVDGLRLDAVPYLYQRDGTNCENLPETHAFLRGLRRHVDANFPGRMLLAEANQWP
                     EDAAEYFGDGDECHMNFHFPLMPRMFMALQMEHRTPIVDILEQTPALPEGGQWATFLR
                     NHDELTLEMVTDEERDYMYRAYANDPQMRINVGIRRRLAPLLGNDRRRIELLNAVLYS
                     LPGTPVLYYGDELGLGDNVYLGDRDGVRTPMQWSPDRNAGFSTASPHRLYLPLVVEPG
                     YHYENVNVEAQQSNSASLLWWMSQLISLRKQHDVLGRGSVRFLDPDNHRVLALVRELD
                     GHPPFLVVANLSRHAQPVELDLSEYAGRRPREVLGHTSFPPIGDLPYFLTIAPYGFYW
                     FSLEADEGAVDADAPLPRLDVAWPDALQRDRRGLERALVAFVGRSRWFAGRSRGLRSA
                     TITDTVRLGSSSDGATTLLVVDVAHRDGSSDSYHVPVVAADAAPPGAVIAELPDGRVL
                     VDAMASPVGITDLACATTQRRIKGDRVELRGAAGRTLRAALADGAPARWAGREQTNSS
                     AFLGDAAVIKLIRRIEDGTNPELEIGGFLTDEAAFAHSPPVIGSLELRRDVARNGSDP
                     EPGTIAVVTELVPHDDDAWTSTADELARFFDRAIGSGEKRPDPVRCADLLGVA"
BASE COUNT         1406 a         3316 c         2907 g         1358 t
ORIGIN      
        1 acaggtgccc ggcacgcacc gcgtccgcgg ttgcttccaa gttgtcgagg tggcctgatg
       61 tttcgaggtc ggcacgcgcc cgccccgtcg tggtgccttg ttgggctgcg agccagtccg
      121 cggcggacgc gtatccttgg cgttcccact cccgtgcttg ggcggcacgc gcggtcatga
      181 gtactcgacc cgactcggcg acccgctgta cgtcggcgaa cgcggccagc acctccggag
      241 caaacggcag cggcacatcg tttggccgca actccgacac cactgcgcgc aacgccacgc
      301 cgatcgcggc gatgtccgaa gcgagttgac tcttcggccc ttcgccggga tgcgaactgg
      361 tgttcgtgac catgtcaaca gtcaagcacg gacccctgac agtcagatcg aggtgccggg
      421 tcggtcgcag ctcgggacga aatcaggtcc cctgcccgaa cgggggatgt gccgtttgct
      481 gagcccggga cccgggtaga gaagcgcacc atgacgacga ccgatccccc ggccacccct
      541 cccgtcgaga tctggcccgg tcgcccggcg ccactcggag cccactggga cgggcagggc
      601 acgaacttcg cagtgttctc cgagcaggcc gaatccatcg acctgtgcct gttcgacctc
      661 gacggagaag ccgccggcac cgagacctgc atccccatcg aggaggtcga ctcccacacg
      721 tggcacgtgt acctgccggg cgtcggcccc ggcacccact acggctaccg cgtgaccggc
      781 ccgtggaacc ccgacggggg ccagtggttc aaccacaaca agctcctgct cgacccgtac
      841 gccaaggcga tcgagggcga ggtcgcctgg gacgaggcgt gcttcgccta cgtccggggc
      901 gacgaggaca cgatgaacga gaccgactcg gcaccgttcg tgccgcgctc ggtcatcgcg
      961 acgccctggt tcgactggtc gaccgatcgt ccgcctgaca cgccgatgga gcgcagcgtc
     1021 atctacgagc tgcacgtgaa ggggttcacc caactgcacc cggccgtgcc cgaacaccta
     1081 cgcggcacct acgccggcct ggcccacccc gaggtcatcg ggtacctcac cgatctcggc
     1141 gtcacggcgg tcgagctcca gccggtgcac cacttcatcc acgactccca tctcgtcgag
     1201 cagggcctcc gcaactactg ggggtacaac tcgatcgggt tcttcgcccc gcacgccggc
     1261 tactcctcgg cgacccgcgc cgaggacgcc gccggcgagt tcaagtcgat ggtgcgcgac
     1321 ttccaccagg ccggcctgga ggtgatcctc gacgtcgtgt tcaaccacac cgccgagggc
     1381 aaccacctcg gaccggtgct ctcgttcaag ggcctcgaca accacgcgta ctaccggctg
     1441 atggacgacg agccgaagta ctaccgcgac ttcacgggca cggggaactc cctcaacatg
     1501 cggcacccca gttcgctgca gctcgtcatg gacagcctcc gctactgggt gaccgagatg
     1561 cacgtcgacg ggttccgttt cgacctcgcc gcggcgctgg cccgcgagct ctacgacgtc
     1621 gaccgactgt cggcgttctt cgacctgatc cagcaggacc cgatcatcag ccgggtgaag
     1681 ctcatcgccg agccctggga cgtcggcgag ggcgggtacc aggtcggcaa cttcccgccg
     1741 ctgtggtcgg agtggaacgg caagtaccgc gacacgatcc gcgacctgtg gcgtggcgag
     1801 ggcggggtgc tcggcgacgt cgccacccgg atggccggca gttccgacct gtactcgtgg
     1861 gagcgcggca gccccaccgc cagcatcaac ttcgtgaccg cccacgacgg gttcacgctg
     1921 cgcgacctcg tcagctacaa cgagaagcac aacgaggcga acggcgagga caacaacgac
     1981 ggcgagtccc acaaccgttc gtggaactgc ggcaccgagg gcccgaccga cgaccccgac
     2041 atccttcgcc ttcgaggtca acagctgcgc aacctcctcg cgacgttgct gatgtcgcag
     2101 ggggttccca tgttgctggc cggcgacgag ctgggtcgca cccagcaggg caacaacaac
     2161 acctattgcc aggacaacga gctcaactgg atcgactgga ccctcctcga cgaggacgcc
     2221 gatggtggcg gtcctgatgc agggagcggg aaggggttgt gggaggcgac gaagcaactg
     2281 atcgcgctgc gcgccgagca cccggcgttg cgccgtcgcc gctggttgac cgggcacgag
     2341 gacggggagg ccgcggttcg ctggctccat cccgacggcc acgacatggc cgacggtgac
     2401 tgggacggtt cgagcgagat ggcgatgctg ctcgacggga cggtcatccc cgaacgaggg
     2461 ccccgcggtg aggtgatccg cgacgaccgc atcctgctgc tgatcgacgc cggcagcgag
     2521 ccgaccgatt ggtcgctccc gccgacgccc ggatcgtggg tcgagcagtt cagctcggcg
     2581 ctgggctcgc agctcaacga cgacggcacg tatcgcacac cgggccggag cgtcgtggtg
     2641 ttcgtcgaag cggcggacta ggtgccagcc cctgacgttc gctggttgcc cgaaggcatc
     2701 ggcggtcccg cgctcgagga caacccggct gccttcgagt ggaccgacga cgggttccgc
     2761 cccgagccct ggccgcacgc cgtcctctac gagctccacc tcggcacgtt ctcgccgtcg
     2821 gggaccgccg ttgcggcgat cgaccacctc gaccatcttg tcgagctggg cgtgacccac
     2881 gtcgaggtga tgccgctcgg tacctacggt ggccggtggg ggtggggcta cgacggcgtg
     2941 tactggtcgg cgccgcagca cacctacggc tcgccgaacg acctccgggc gttcgtcgac
     3001 gcctgccacg cccgagggct cggcgtgatc gtcgacgtgg tctacaacca cctcgggccc
     3061 gtgggtgccg acgatcccgg gttcgagccc ttcctcaccg acgcccacca cacgccgtgg
     3121 ggcaaggcga tcaacctcga cggaccgggc tcgcgggtcg tccgcgatcg catcgtcgac
     3181 gacgccacca tgtggatcgt cgactatcac gccgacggcc tccgcctcga cgccatccac
     3241 gcgctcgtcg atgactcgcc cgagcacctg ctggccgagc tgagccgcac cgtcgagggc
     3301 cttggcctcg accgcgaggt tgtgctgatc gacgaggacg aacggcccga ctcgacgccg
     3361 gtacggccgc gtaccgatgg cgggtacggg ctgaccgcca agtgggccga cgagctgcac
     3421 cacgcggtgc acgcgtacct gaccggggag cggcacgcct actacgagcc gtacggcgac
     3481 ccggagctga tcgggaagga gctggcgtcg ggcgctccct ggaaggtcgt gtcgctccag
     3541 aaccacgatc aggtgggcaa ccggcccttc gccgatcgcc tgcaccagac cacctcgatc
     3601 gagacggtgc tcacggtgct cccgctgttc ctgctgggcc cgtcggtgcc gatgctgttc
     3661 cagggcgagg agtgggcggc gtcgacaccg ttcccgtact tcgccgacca ccacggcgaa
     3721 ctggccggcg cgatccgcga gggccgcgct cgggagttcg cgtgggtcgc cgccgaagac
     3781 gggccgccgt ccttcccgaa cgggctgccc gacgccaccg ccgagtcgac ctaccggtcg
     3841 gcgctcctgc gttggggcga gatcgcggca ccggagcacg ctgccgtgtt ggatcgctac
     3901 cgggagctga tcgccctccg ccggtcgttg ccccatgtgt tcggcgccga cgccccgtgc
     3961 gtcatcgacc aggagggcgc cgtgatcacg atgcgccgcg gtggcagcgc cggcgaccgc
     4021 ggcgaagggc cgggcgtcgc caccgtcgtg tgcgacctcg acgccccgag ggtgacgatc
     4081 accggcgtct gacgccggtg cgacctaccg cctcaccctc gtgggggatt ctcgatccgg
     4141 gggagatcgg gtagcagacc cccgtgattc gacgaacgaa gagccgaacc cccaacagcg
     4201 tcaaggtctc cttcgcattg ccgctcgacg tcgcaccgaa cccggtgtcg gtcgtcggcg
     4261 acttcaacga ttgggatccg accgcccatc ccctccgcaa gcgcagcaac ggaacccggt
     4321 ccgtcaccgt tgagctggcc gaaggcgagt ccgtgcagtt caagtatctc ggcgacaacg
     4381 gggactggtt ccccgagcac gaggccgacg gcgccgtcgt caacgagtgg ggcgaggtca
     4441 actctcgcct cgacctgtga gcggtcggca ctcctcgacg aaggtccgtg acggtggcgt
     4501 cgccgacccg tcgagtgagc ctgcaccggc gccgacccgg cttgccatcg aggtcatcca
     4561 tccgatcgtc gacgccgccc gattcactgc gaaggcgtcg gtcatcgagc ccgtcgagct
     4621 cgtcgccgac gtgttcgccg acggtcacga ccacctcgct gcctccgcca cgttccgcca
     4681 caccggcttc ccggtggaga ccgacatcga cctcgtgccg gtcggcaacg accgttggac
     4741 cggctcggtg gccctcgccc ggatcggccg gtgggagttc accgtgtccg cctggatcga
     4801 cccgtgggag tactggcggc gctcgatcgc agcccgtcag gccgccggtg ccgacgtctc
     4861 ggccgaactg ctcgacggtg ccgcgttgct gcggcgagcc gctgacgccg ggttcgatgt
     4921 gcccctcgac gtcgacccgg cggccacgtc ggccgacccc gacgtcgcga ccacgatgcg
     4981 atcgtgggcc cggacactgc cgggcggaac gaccacacca ccgctcccgt tggaggtggc
     5041 gcgtgagcgt gccgcgtacg gcgcctggta cgagctgttt ccccgctcca ccgtcgtcgg
     5101 tggtcccgag cacgaacacg gaaccctcgt cgatctcgtc gatcgcctcg gccacgtggc
     5161 cgacctcggc ttcgacgtcg tgtacctccc gccgatccac cccatcggcc tgaccgcccg
     5221 caagggaccc gacaaccgcc tgaccgccga tcctgccgac cccggcagcc cctgggcaat
     5281 cggcgccgcc gacggcggac acacggcggt gcaccgcgag ctcgggacga tcgccgactt
     5341 cgaccgcgtc gtcgcggcag cgaacgaacg cggcctcgag atcgccctcg acctcgcgtt
     5401 ccagtgctcg cccgaacacc cgtgggtgac cgaacatccc cagtggttcg cccgccgcgc
     5461 cgacggcacg atccgaaccg ccgagaaccc gcccaagcgc tacgaggaca tctatccgat
     5521 cgacttctcg tgcgcggact gggagtcgct gtggaccgcc ctcgccgacg tcgttcgtca
     5581 ctgggtcgac cacggcgtga agatcttccg ggtcgacaac ccccacacca aaccgttcgc
     5641 cttctggcag tggttgatcg gcgagatccg cgccgaccat cccgacgtcg tgttcctcgc
     5701 cgaggcgttc acgcggcccc gggtgctgga acgactcgcg aaggtcggct tcgaccagag
     5761 ctacggctac ttcacgtgga agcgcacgcc gaccgagctg cgggagtacc tgcgcgacct
     5821 cgacgaggtc gccgacttcc ttcgccccaa cctctggccc aacacccccg acatcctgac
     5881 cgagcagctg cagaacgggg gccgccctgt cttcgagagc cgagcggtgc tcgccgcgtt
     5941 gctcgccgcc aactacggcg tctacggccc cgcgtacgag ctgctcgagg cgcaaccggt
     6001 tcggcggccg agcgaggact acgcgtcgtc ggagaagtac gaggtccgcc gctgggacct
     6061 cgacgagccg cagtcgatcg ccccgctgct gcgccggctc aacgagatcc gtgccgccca
     6121 ccccgcgctc cactcggatc gtcgccgggt cttccaccac tgcgacgacc ccggggtgct
     6181 ggcatggtcc aagcgctcgt tcgacgggcg cgacgtcatc ctcgtgatcg tcaacgtcga
     6241 ggccgacctg acccggtctg ggatggtccg tctcgacctc gacgcgctcg gcctcccgcc
     6301 gggcgccacc ttcaccgtcg ccgacctcct gagcggcgag atctaccagt ggcagggcgc
     6361 cgacaactac gtcgcgctcg atccccatcg acgggttgcc cacgtcttcg aggtcacggc
     6421 ctcggaagga acctcatgac cgcagtgctc cgccccccga cgcccccgtc ccccaaggtg
     6481 ccccggcccg ccccggtacc cgcgttggtc ggcacggccg acgaccccga gtggtaccgc
     6541 agcggggtga tctacgagat gcacgtgcgg gcgttcgccg acagcaacgg cgacggtatc
     6601 ggcgatttcc ccgggctcac cgcccacctc gactacgtcg ccgagctcgg cgtgacggcc
     6661 atctggctgc tgccgttcta cccgtcaccc ggtcgcgacg acggctacga catcgccgac
     6721 tacatgtcgg tcaaccccga ctacggcacc ctcgcgcagt tcaagaagtt cctgaaagct
     6781 gctcacgatc gtggcatcag ggtcatcacc gagctggtcg tgaaccacac ctccgaccag
     6841 cacccgtggt tccaacgcgc ccgccgtgcc ccggccggct cgcggtggcg caacttctac
     6901 acgtggtccg acgacgactc ccgctggccc gacgctcgca tcatcttctc cgacaccgag
     6961 gcgtccaact gggcgtggga cccggtggcc ggccagtact actggcaccg cttcttcagc
     7021 caccagcccg acctcaacta cgacaacccc gaggtgcaga cggccatcac gaaggtcgtc
     7081 gacttctggc tcggcatggg cgtcgacgga ctccgcctcg acgcggtccc gtacctgtac
     7141 cagcgggacg gcacgaactg cgagaacctc cccgagaccc acgcgttcct tcgtgggctg
     7201 cgccgccacg tggacgcgaa cttccccggc cgcatgctcc tcgccgaggc gaaccagtgg
     7261 cccgaggacg ccgcggagta cttcggcgac ggcgacgagt gccacatgaa cttccacttc
     7321 ccgctgatgc cgcggatgtt catggcgctc cagatggagc accgcacgcc catcgtcgac
     7381 atcctcgaac agacgccggc cctccccgaa ggcgggcaat gggcgacgtt cctgcgcaac
     7441 cacgacgagc tgacgctcga gatggtgacc gacgaggagc gcgactacat gtaccgggcc
     7501 tacgcgaacg atccgcagat gcgcatcaac gtcggcatcc gacgacggtt ggcgccgctc
     7561 ctgggcaacg accgtcgtcg gatcgagctg ctcaacgccg tgctgtactc actgcccggc
     7621 acgcccgtcc tgtactacgg cgacgagctg ggtctcggcg acaacgtgta cctcggcgac
     7681 cgcgacggtg tccggacccc gatgcagtgg agccctgacc gcaacgccgg cttctccacc
     7741 gccagccccc accgcctcta tctgccactc gtcgtcgaac ccggttacca ctacgagaac
     7801 gtcaacgtgg aggcccagca gtccaactcg gcgtcgttgc tgtggtggat gagccagctg
     7861 atctcgctgc gcaagcagca cgacgtgctc ggccgggggt cggtgcgctt cctcgacccc
     7921 gacaaccatc gtgtgctggc gctggtgcgc gagctcgacg gccatccgcc gttcctggtg
     7981 gtcgcgaacc tctcccggca cgcccagccc gttgagctcg acctgtcgga gtacgccggt
     8041 cgccgccccc gcgaggtgct gggtcacacg tcgttcccgc cgatcggaga cctgccgtac
     8101 ttcctgacga tcgcgccgta cgggttctac tggttctccc tcgaggcgga cgagggcgcg
     8161 gtcgatgccg acgccccgct gccccgactc gacgtcgcat ggcccgatgc cctgcagcgt
     8221 gaccgtcgtg gtctcgaacg agcgctggtc gcgttcgtgg ggcggagccg ctggttcgcc
     8281 ggtcgcagtc ggggacttcg gtccgccacg atcaccgaca ccgtgcggct ggggtcgtca
     8341 tcggacgggg cgaccacgct cctcgtcgtc gacgtcgctc accgcgacgg ttcgtcggac
     8401 agctaccacg tgcccgtggt ggcggccgac gctgccccgc caggagccgt catcgccgaa
     8461 cttcccgacg gacgtgtgct ggtcgacgcc atggcgtccc cggtgggtat caccgacctg
     8521 gcctgcgcca cgacgcagcg ccggatcaag ggcgatcgtg tcgagctgcg cggcgccgcc
     8581 gggcgcaccc tgcgcgccgc gctcgccgac ggcgctcccg cccgctgggc gggtcgggag
     8641 cagaccaaca gctcggcgtt cctcggcgac gccgcggtga tcaagctgat ccgccgcatc
     8701 gaggacggca cgaaccccga attggagatc ggcgggttcc tcaccgacga ggccgccttc
     8761 gcgcactcgc cacccgtgat cggatcgttg gagctgcgac gcgacgtcgc ccggaacggg
     8821 tcggaccccg agcccggcac catcgccgtc gtcaccgagc tcgtgcccca cgacgacgac
     8881 gcctggacat cgaccgccga cgaactggct cggttcttcg accgcgccat cggatcgggc
     8941 gagaagcgtc ccgaccccgt gcgatgcgcc gacctgctcg gcgtggc
//
LOCUS       DAOSOA010000112         8925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7796_length_8925_cov_8.833822, whole genome shotgun sequence.
ACCESSION   DAOSOA010000112 DAOSOA010000000
VERSION     DAOSOA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7796_length_8925_cov_8.833822"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..942)
                     /locus_tag="PKY13_08635"
     CDS             complement(115..942)
                     /locus_tag="PKY13_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOT80041.1"
                     /translation="MTTVDEPTNTPAAPASHAAMLNLTLRVWRQDGPDSPGEFETYTT
                     TIADDASFLEMLDYVNEDLIANDREPITFDHDCREGICGTCGVMINGQAHGPQKATAT
                     CQLHMRQFKSGDTIVIEPWRAAAFPIVKDLMVNRAAFDRIIESGGYITVSTGSAPEAN
                     LTPVPKDAADAAMDAAACIGCGACVAACPNSAAQLFTSAKMQHLNMLPQGQAERWTRA
                     ENMVETMEQFFGSCTNHGECQEACPKEITIDFIAMMNRDYVKAKMANRRRGGQRKAG"
     gene            complement(957..2894)
                     /locus_tag="PKY13_08640"
     CDS             complement(957..2894)
                     /locus_tag="PKY13_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOT80042.1"
                     /translation="MTLTLDSKVPGGPIADMWENYKFDLKLVNPANRRKFEVIVVGTG
                     LAGGAAAATLGELGYNVKVFTYHDSPRRAHSIAAQGGINAAKNYRGDGDSVHRLFYDT
                     VKGGDYRSREANVHRLAEVSVNIIDQCVAQGVPFGREYGGLLDNRSFGGSQVARTFFA
                     RGQTGQQLLLGAYQALAHQIHLGTCTLYNKMEMVEVVVDPETGLATGIIARDLISGEL
                     TRHSAHAVVLATGGYGNVYFLSTNAKNSNATAAWRAHRKGAAFANPCYTQIHPTCIPA
                     ADEFQSKLVLMSESLRNDGRIWVPSNIDDCGKQASEIPETDRDYYLERKYPAFGNLVP
                     RDIASRNAKTMVDQGHGIGPLRNGVFLDFSEAIGRVGLDTIKARYGNLFDMYERITGE
                     NPYETPMRIYPATHYTMGGLWVDYNLQTTIPGLFAIGECNFSDHGANRLGASALMQGL
                     ADGYFVLPSTIAGYLAPQLNKKPMATDAAAFDQAEADARGRIDQLMGARGTKSVDHYH
                     RELGKIIWDYCGMARNAHDLEKALSEIPVLREEFRKDVKILGTAEGVNQSLEKALRVD
                     DFMELAELKVRDALHREESCGGHFREEHQTPEGEALRDDENFAYVGAWEYAGPNIQKD
                     AVLHKEPLVFESVPLSQRSYK"
     gene            complement(2956..3705)
                     /locus_tag="PKY13_08645"
     CDS             complement(2956..3705)
                     /locus_tag="PKY13_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOT80043.1"
                     /translation="MKRPGADAPVINPPAARRAPWPVQFYRSAVGKKWVMAISGIMML
                     GFVVAHLVGNLKLYLGKEPDGKYAIDVYGEALRELLHPIMPNMVVLWILRIGLIVALV
                     AHVHAAATLTIMNRKARPTSYQSSRDYVAVNFASRSMRYTGVILLAYLVFHLLDLTIG
                     SANPDFVDGAVHNNMIASMQRWPVAIAYIIANIAIAVHLYHGTWSMFQSLGLNNPRYN
                     AARKAFATGISLLIGVGNVLFPILIVTRVVK"
     gene            complement(3802..4494)
                     /locus_tag="PKY13_08650"
     CDS             complement(3802..4494)
                     /locus_tag="PKY13_08650"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT80044.1"
                     /translation="MTAPTEGFRQHRFAPAPGATRVLLVRHGESAPAHPDRPFPLADG
                     HGDPPLHPDGELQAQAVGRRLAETEAVDAIYVTTLQRTHQTAAPLAALTGLSPAVEPD
                     LREVHLGEWEGGELRVRAAAGDPAFVAAMAAQRWDLIPGAEPLEDFDRRLQDGLSRIV
                     AAHPDQTVVAVVHGGVIGQLLAHAVGVEGFAFVGADNASISELVVHDGRQVLRRFNDT
                     AHLDAPPNVTSV"
     gene            complement(4523..5671)
                     /locus_tag="PKY13_08655"
     CDS             complement(4523..5671)
                     /locus_tag="PKY13_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80045.1"
                     /translation="MPRPTSVTVDRGIVRIHGRRRFLSGIRLTPRGTLISGFDVDPHS
                     AVSVVAATGANAVVVEPDDATPELLDAANDCGVLVLVDVTGIDDPRRRSAAAGVAGWF
                     VEGRGWRPKRPADRAGPPIVSAPRPWALGWATGDERDLPDRMRRARRPPTALTGFGAP
                     SVAATDAWFDGAGRAWRRLGEHDGPPVGERDLACLLRHSPPAAFDDPHDWAAATRAYQ
                     AMVIRFHVETARRLRYSPLGVVVPEALYDPAGGVSSALIGPDGTPKEALRALTDAYHP
                     VTVIADRLPPHLHGHEALAIDVHVVNDLAEPLADASVVATLTWADGRQRYGFGGGAAP
                     DSVQRVGTIPIVVPPVSGPLDLVIELRHGDAVVTRTYRSEIFADPHEH"
     gene            complement(5804..7417)
                     /locus_tag="PKY13_08660"
     CDS             complement(5804..7417)
                     /locus_tag="PKY13_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOT80046.1"
                     /translation="MVALVATACPEQPGGGGTTTTTAPTTTSTTTTTLADSDGDGYTT
                     GQDCNDNDPAVNPGQAVDTVGDGVDSNCDGQDGIATNTVFVSNTGADSSTCGDIAAPC
                     ATIDQGIVRAGALSRTQIQVAEGTYAKFDVVAGLEIGGHYDSATWTKVGAGDTVVTAS
                     FDASVNGPVGITADGIATPTKLADLVVNGVVAGAGLASYGVIVRNSTSALVFDTVKIN
                     GGTGGSGSAGANGISASSAPAASGMGGGGGFEPSGTYNNTDSGAGGGGAGGGGVGGNG
                     GVVDASTSVFGNHDAQAGQQGGIGAGGAAGGTGGQPDTTALIGCGSNGLPTPGGAGTA
                     GVAGTNGTVGTGGSASAGAVSGGLWTASASSAGGNGGAGGPGRPGGGGGGGGASDCDS
                     DDAGAGGGGGGAGGAGAFSGGGGGGAGAASIALLLDNSSPTLTGVQIVLGTGGNGGNG
                     GNGAFGQPGGTGGTGGPGFEDGGAGGAGGNGGNGGTSGAGGGGAGGPAIGIVRQGSAT
                     GTATFSGGSGGAGGTNGGATGTVTNSVTL"
     gene            complement(7534..8847)
                     /locus_tag="PKY13_08665"
     CDS             complement(7534..8847)
                     /locus_tag="PKY13_08665"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOT80047.1"
                     /translation="MVEGSGACWFGAPGFEVLSVADDGAELVIEVETTASTVGCGRCG
                     VRARAKDRRWVTVRDAPSGSRAVLVRWRKRVWACPDAECPAKTWTELSPLVAPRRVLT
                     ARAAEWATGRVASVEGTPASIAAQFGVSWSTVWTAVERIGRQRVEDPDRVGPAAMVGF
                     DETVMQPAHRRRRRRFITAVVDVGTGQILDVFEGRDARDLRAWMAAMPPDWLARIEVV
                     SVDPHEGYRSAVTGPTSPLRDVTIVVDPFHIVRLGNAAVTRCRQRVQAETLEHRGWKG
                     DPLYDVRKLLLMGAERLDDNGWDRLHRALRDGDPDGEVRDCWVAKEKLRDVYLTNDPD
                     EAAGRLDDVIVWCSDADSGPELRRLAKTLERWRQQILNRHSTGASNGPVEAANLLIKQ
                     VKRSGRGFRNLDNYRLRILLAGGTNPMRETQQVTSIRPRRPRLVA"
BASE COUNT         1287 a         2981 c         3208 g         1449 t
ORIGIN      
        1 ccttcagccg ccttgccgat ggtgatcttc ccatcgccac tgctgcaccc cacgagggtg
       61 atgcagagcg tggcgacggc gagggcggtg aaacgggtgc gcatgaacgg aacgctagcc
      121 cgccttgcgt tgtcccccgc ggcgacggtt cgccatcttt gccttgacgt agtcacggtt
      181 catcatggcg atgaagtcga tcgtgatttc cttggggcag gcttcctggc actcgccgtg
      241 gttggtgcag ctgccgaaga actgctccat ggtctcgacc atgttctcgg cgcgggtcca
      301 ccgctcggcc tgaccctgcg ggagcatgtt caggtgctgc atcttcgccg aggtgaagag
      361 ctgtgccgcc gagttcgggc acgccgcgac gcaggcgccg cagccgatgc acgccgcagc
      421 gtccattgct gcgtccgccg cgtccttcgg gaccggcgtg aggttcgcct cgggtgcgga
      481 gcccgtgctc acggtgatgt aaccgcccga ctcgatgatg cggtcgaagg cagcgcggtt
      541 gaccatgagg tccttgacga tcgggaaggc cgcggcgcgc cagggttcga tcacgatcgt
      601 gtcgccggac ttgaactgcc gcatgtgcag ctggcaggtg gccgtggcct tctgcggccc
      661 gtgggcctgg ccgttgatca tcacgccgca ggttccacag atgccctcgc ggcagtcgtg
      721 gtcgaacgtg atgggctcac ggtcgttggc gatcagatct tcgttgacgt agtcgagcat
      781 ctcgaggaac gacgcgtcgt cggcgatggt ggtggtgtag gtctcgaact caccggggga
      841 gtcggggccg tcctggcgcc agacccggag cgtgaggttg agcattgccg cgtgcgatgc
      901 gggcgcagcg ggagtgttgg tgggttcgtc gacggtcgtc atcgggggct tcctgcctac
      961 ttgtagctgc gctggctgag cgggacggat tcgaacacga gcggctcctt gtggagcacg
     1021 gcgtccttct ggatgttggg cccggcgtac tcccaggcac cgacgtacgc gaagttctcg
     1081 tcgtcgcgga gcgcttcgcc ctcgggggtc tgatgctcct cgcggaagtg gccgccgcac
     1141 gactcctcgc ggtgcaaggc gtcgcggacc ttgagctcgg cgagctccat gaagtcgtcg
     1201 acccggagcg ccttctcgag ggactggttg accccttcgg ccgtgccgag gatcttgacg
     1261 tccttgcgga actcctcgcg aagcacgggg atctccgaca gggccttctc gagatcgtgg
     1321 gcgttgcggg ccatgccgca gtagtcccag atgatcttgc cgagctcgcg gtggtagtgg
     1381 tcgaccgact tggtgccgcg ggcgcccatc agctggtcga tgcgaccgcg ggcgtcggct
     1441 tccgcctggt cgaacgccgc ggcgtcggtg gccatcggct tcttgttgag ttgcggtgcc
     1501 aggtagccgg cgatggtgga cggcagcacg aagtagccgt cggccaggcc ctgcatgagc
     1561 gccgacgcac cgaggcggtt ggcgccgtgg tcgctgaagt tgcactcgcc gatggcgaac
     1621 aggccgggga tcgtggtctg caggttgtag tccacccaca ggccacccat cgtgtagtgg
     1681 gtcgcggggt agatgcgcat gggcgtctca taggggttct cacccgtgat gcgctcgtac
     1741 atgtcgaaga ggttgccgta gcgcgccttg atggtgtcga gcccgacgcg gccgatggcc
     1801 tcgctgaagt cgaggaacac accgttgcgg agcgggccga tgccatggcc ctgatcgacc
     1861 atggtcttgg cgttgcggga cgcgatgtcg cgagggacga ggttgccgaa ggccgggtac
     1921 ttgcgctcga ggtagtagtc gcggtccgtt tcggggatct ccgacgcctg cttgccgcag
     1981 tcgtcgatgt tgctcggcac ccagatgcga ccgtcgttgc ggagcgactc ggacatgagc
     2041 acgagcttcg actggaactc gtcggcggcc ggaatgcagg tcgggtggat ctgcgtgtag
     2101 caggggttgg cgaaggccgc gcccttgcgg tgcgcccgcc acgctgcggt ggcgttggag
     2161 ttcttggcat tggtcgacag gaagtagacg ttgccgtagc cgccggtcgc caacaccacg
     2221 gcgtgtgccg agtgccgggt cagctcaccg ctgatgaggt cgcgggcgat gatgcccgtg
     2281 gcgagaccgg tctcggggtc gacgacgacc tcgaccatct ccatcttgtt gtagagggtg
     2341 caggtgccga ggtggatctg gtgggcgagc gcctggtagg cgccgagcag cagctgctgg
     2401 ccggtctggc cgcgggcgaa gaacgtgcgg gccacctgcg aaccaccgaa cgatcggttg
     2461 tcgaggaggc cgccgtactc gcggccgaac ggcacaccct gggcgacaca ctggtcgatg
     2521 atgttgaccg acacctcggc gagacggtgc acgttggcct cgcgggagcg gtagtcgcca
     2581 cccttgaccg tgtcgtagaa gaggcggtgg acgctgtcgc cgtcgccgcg gtagttcttg
     2641 gcggcgttga tgccaccctg cgccgcgatg gagtgcgccc ggcgggggga gtcgtggtac
     2701 gtgaagacct tgacgttgta gccgagctcg ccgagcgtcg ccgctgcggc gccaccggcg
     2761 aggccggtgc ccaccacgat gacctcgaac ttccggcggt tcgccgggtt cacgagcttg
     2821 aggtcgaact tgtagttctc ccacatgtcg gcgatcggac cgccggggac cttcgagtcg
     2881 agcgtaagtg tcatgtcggt tcctgggtcg gttcgaacga ggtggttgct cgggtcactt
     2941 caccacttgc gtggttcact tcaccactcg cgtgacgatg agaatgggga acagcacgtt
     3001 gccgacaccg atgaggagcg agatgccggt ggcgaaggcc ttgcgtgcgg cgttgtagcg
     3061 ggggttgttg aggcccaggc tctggaacat cgaccaggtg ccgtggtaca ggtgcaccgc
     3121 gatggcgatg ttggcgatga tgtaggcgat ggccacgggc cagcgctgca tcgaggcgat
     3181 catgttgttg tggacggcgc cgtcgacgaa gtcggggttg gccgagccga tcgtgaggtc
     3241 cagcaggtgg aacacgaggt acgccaacag gatgacgccg gtgtagcgca tcgaccgtga
     3301 cgcgaagttg accgcgacgt agtcgcgcga cgactggtag ctcgtgggcc gcgccttgcg
     3361 gttcatgatc gtcagggtcg ccgcggcgtg gacgtgggcg accagcgcca cgatcaggcc
     3421 gatccgcagg atccagagca ccaccatgtt gggcatgatc gggtgcagca gctcgcggag
     3481 cgcctcgccg tacacgtcga tcgcgtactt cccgtcgggc tccttgccca ggtacagctt
     3541 gaggttgccg acgaggtggg cgacgacgaa gccgagcatc atgatgccgc tgatcgccat
     3601 gacccacttc ttgccgactg ccgatcggta gaactggacg ggccacggtg cccgtcgggc
     3661 ggccggggga ttgatcaccg gagcgtcggc tccgggtcgc ttcatcgttt gcgccatgtg
     3721 cggtgagctc ccactcgacg ggtttccctt acgtgagaaa cctatcgtcc acgtcggcga
     3781 cagcggaaac tgagacgttg ctcagacgga cgtcacattc gggggagcgt cgaggtgcgc
     3841 cgtgtcgttg aagcggcgca gcacctggcg gccgtcgtgg acgacgagct cggagatcga
     3901 tgcgttgtcg gcgccgacga acgcaaagcc ctcgacgccg acggcgtggg ccaggagctg
     3961 gccgatcacg ccgccgtgga cgacggccac cacggtctgg tcggggtggg ccgcgacgat
     4021 gcgcgagagg ccgtcctgga ggcggcggtc gaagtcctcg agcggttctg caccgggaat
     4081 gaggtcccat cgctgcgccg ccatcgccgc gacgaacgcc gggtcgccgg cagccgcccg
     4141 gacacgcagt tcgcctccct cccactcacc gaggtgcacc tctcggagat ccggctcgac
     4201 ggccgggctg aggcccgtga gcgcagccag cggggccgcg gtctggtggg tgcgctggag
     4261 ggtggtgacg tagatggcgt cgacggcttc ggtctcggcg agccgccgtc ccactgcctg
     4321 cgcctgcagc tcgccgtcgg ggtggagggg cggatcgccg tggccgtcgg cgagcgggaa
     4381 gggccggtcg ggatgggccg gcgccgattc gccgtggcgg acgaggagca ctcgcgtggc
     4441 cccgggggcg ggagcgaagc ggtgttggcg gaagccttcg gtcggtgcgg tcacgggtga
     4501 gctcctgagt gggcggggcg ggtcagtgtt cgtgggggtc ggcgaagatc tcgctgcggt
     4561 aggtccgcgt gaccacggca tcgccgtggc gaagctcgat gacgagatcc agtgggccac
     4621 tcaccggggg gacgacgatc gggatcgtgc ccacgcgttg cacgctgtcg ggggcggctc
     4681 caccgccgaa tccgtagcgt tggcggccgt cggcccacgt cagcgtggcg acgaccgacg
     4741 cgtccgcgag gggctccgcc agatcgttca cgacgtggac gtcgatggcc agcgcctcgt
     4801 gcccgtggag gtgcgggggc agccgatccg cgatcacggt gacggggtga taggcgtcgg
     4861 tcaacgccct cagcgcctcc ttcggcgtgc cgtcgggccc gatgagggcc gacgacacac
     4921 cgccagccgg gtcgtagagc gcctccggga cgaccacgcc gagcggcgag taccggagtc
     4981 ggcgggccgt ttcgacatgg aagcggatca ccatcgcttg ataggcccgc gtcgctgctg
     5041 cccagtcgtg ggggtcgtcg aacgctgcgg ggggactgtg ccggaggagg cacgcgaggt
     5101 cccgctcgcc gacgggggga ccatcgtgtt ctccgagccg gcgccacgct cgcccggcgc
     5161 cgtcgaacca ggcgtcggtc gcggcgaccg acggtgcccc gaacccggtg agtgccgtcg
     5221 gtggtcgacg cgctcgccgc atgcggtcgg gcaggtcgcg ttcgtcgccg gtcgcccatc
     5281 cgagtgccca gggtcgtggc gcggacacga tcggcggacc ggcccggtca gccggtcgct
     5341 tcgggcgcca ccctcgaccc tcgacgaacc aacccgccac ccccgccgcg gcggaccgac
     5401 ggcgggggtc atcgatcccc gtgacgtcga cgagcacgag gacgccgcaa tcgttcgcgg
     5461 catcgagcag ctccggtgtc gcgtcgtcgg gttcgacgac gactgcgttc gctccggtcg
     5521 cagcgacgac cgacacggcg gagtgcgggt cgacgtcgaa ccctgagatc aacgtgcccc
     5581 gtggcgtgag acggatcccg ctgaggaatc gacgtcgtcc gtggatcctg acgatgcccc
     5641 gatcgaccgt cacggaggtg gggcggggca tccgccgact gtagtgagac gttgctcccg
     5701 acggagaccg tggcggggaa cgacgaaccc cgggggttcg ccgagcacgg agctcgacga
     5761 acccccgggg tccgggtcgg tcagatgatg tcgggtgccc cggtcagagg gtgacggagt
     5821 tcgtcaccgt gcccgtggcc ccgccattcg tgccgccggc gccgcccgat ccaccggaga
     5881 aggtcgccgt gccggtcgcc gagccctggc ggacgatacc gatggccggg ccgccggccc
     5941 caccaccgcc ggctcccgac gtgccgccgt tgccgccgtt gccgccggcg ccaccggcgc
     6001 cgccatcctc gaagcccggt ccgcccgtgc cgcccgtgcc gcccggctga ccgaaggcgc
     6061 cgttgccccc gttaccgccg ttgccgcccg tgccgagcac gatctggacg cccgtgaggg
     6121 tgggcgagga gttgtcgagc agcagtgcga tcgatgccgc tccggcgccg ccgccaccac
     6181 caccggagaa cgctccggcg ccaccggcgc cgccgccgcc accaccggcg ccggcgtcgt
     6241 cgctgtcaca gtcggaggcg ccgccgccac caccgccgcc gccgggacga cccgggccgc
     6301 cggcgccacc gttgccaccg gccgacgagg cggatgccgt ccaaaggcca ccgctcaccg
     6361 ctccggccga ggccgaaccg ccggtgccga cagtgccgtt cgttccagcg acacctgcag
     6421 ttccggcgcc gccgggcgtc gggaggccgt tgctaccgca gccgatgagc gctgtcgtgt
     6481 ccggctggcc gccggtgccg ccggcagcgc cgcccgcgcc gatcccgccc tgctggcccg
     6541 cctgggcgtc gtggttaccg aagacgctcg tcgacgcgtc gaccacaccg ccgttgccgc
     6601 cgacgccacc gccgcccgca ccgccgccac cggctccgga gtcggtgttg ttgtaggtgc
     6661 cggacggttc gaagccgccg ccaccaccca tgcccgaagc ggcgggagcc gaggaggccg
     6721 agatgccgtt cgccccagcc gaaccgctcc cgccggtgcc gccgttgatc ttgaccgtgt
     6781 cgaacaccag ggccgaggtc gagttgcgca cgatcacgcc gtacgacgcc aggccggcgc
     6841 cggcgacgac gccgttgacc acgaggtcgg cgagcttggt cggcgtcgcg atgccgtcag
     6901 cggtgatgcc gacgggaccg ttaacggacg cgtcgaacga cgccgtgacg acggtgtcgc
     6961 cggcgccgac cttcgtccag gtcgccgagt cgtagtgtcc gccgatctcg agaccggcga
     7021 cgacgtcgaa cttggcgtac gtgccttcgg ccacctggat ctgggtgcgg ctgagggcac
     7081 cggcgcgcac gatgccctgg tcgatggtgg cgcagggagc ggcgatgtcg ccgcaggtgg
     7141 acgagtcggc gccggtgttg gacacgaaca cggtgttggt ggcgatcccg tcctggccgt
     7201 cgcagttgga gtcgacgccg tcgcccaccg tgtcgacggc ctggcccggg ttgacggccg
     7261 ggtcgttgtc gttgcagtcc tggccggtcg tgtaaccgtc gccgtcgctg tccgcgagcg
     7321 ttgtggtcgt cgtcgacgtg gtcgtcggtg cggtggtggt agtggtaccg ccgccgccgg
     7381 gttgttcggg acacgcagtg gcgacgagcg caaccatccc cagcagcggt aacagagcac
     7441 gacgtacgcg catgatgaac ctcccagttc gtcccaccgc ccctcggtga gggtgcctga
     7501 ctatcggtcg cccggatccg aagcaatggg gccctacgcg accaacctgg gacggcgggg
     7561 tcggatgctc gtgacctgtt gagtttcgcg catggggttg gtgccgccgg cgaggaggat
     7621 gcggagccgg tagttatcga ggttgcggaa ccctcgacct gaacgcttga cctgcttgat
     7681 cagcaggttc gccgcctcga caggaccgtt cgaggcgccg gtgctgtgcc ggttcaagat
     7741 ctgctggcgc cagcgttcca gggtcttcgc gaggcgtctc aactcggggc ccgagtcagc
     7801 atcggagcac cacacgatga cgtcgtcgag acggccggcc gcttcgtcgg ggtcgttggt
     7861 gaggtagacg tcgcggagct tctccttcgc gacccagcag tcccgcacct cgccgtcggg
     7921 gtcgccatca cgcagcgccc gatggagccg gtcccagccg ttgtcgtcga ggcgttcagc
     7981 gcccatcaac aacagcttgc ggacgtcgta gagcgggtcg cccttccacc cgcgatgctc
     8041 gagcgtctcg gcctgcaccc gctggcgaca ccgtgtgacc gcggcgttgc ccaacctgac
     8101 gatgtggaac ggatcgacga cgatcgtgac gtcacgcaac ggactcgtcg gcccggtcac
     8161 cgctgaccgg tagccctcgt gcggatcgac cgacaccacc tcgatccggg ccaaccagtc
     8221 cggcggcatg gccgccatcc aggcccgcag atcacgggca tcacggccct cgaacacgtc
     8281 gaggatctga ccggtgccga catcgacaac agcggtgatg aaccgtcgtc ggcggcgccg
     8341 gtgcgctggt tgcatcacgg tctcatcgaa ccccaccatc gccgccggtc cgacccggtc
     8401 agggtcctcg acacgctgac ggccgatccg ctcaaccgcc gtccacaccg tcgaccacga
     8461 caccccgaac tgcgcggcga tcgacgccgg cgtcccctcc acgctggcga cccggcctgt
     8521 ggcccactcc gccgcccggg ccgtcagcac ccgccgcggt gcgaccagcg gtgacaactc
     8581 ggtccacgtc ttcgccgggc actccgcgtc ggggcaggcc cacacgcgtt tgcgccaccg
     8641 gaccagcacc gccctgctgc ctgacggggc gtcacgcacc gtcacccacc gccggtcctt
     8701 cgcacgggca cgcacaccgc accggccgca cccgaccgtc gacgcggtcg tctcaacctc
     8761 gatgaccaac tcggcaccgt catcggcgac gctcagcacc tcgaaccctg gcgcgccgaa
     8821 ccagcacgca ccactacctt caaccaacgg gggcctccct cgtctctgac cgtcaagcag
     8881 tcacgagatt tgtggaggcc cctccatcac cggtggaccc cgacc
//
LOCUS       DAOSOA010000113         8811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7927_length_8811_cov_5.513362, whole genome shotgun sequence.
ACCESSION   DAOSOA010000113 DAOSOA010000000
VERSION     DAOSOA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7927_length_8811_cov_5.513362"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKY13_08670"
     CDS             <1..561
                     /locus_tag="PKY13_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80048.1"
                     /translation="HCTVVASSLTGAYATKAIADSIRPGDTPADTPRLVLVTPTGLGD
                     AQRRRGGLVARAAYAVGRHTPIGDLAVAALTSGPSVRWFQRNKTYSNPEALHEDEVVE
                     TRRAGRLPNGKHLQLAFVTGRLALPLSRADVRAARPLVVWARGQAFSEDSDMALWQAA
                     GATVVAVPSGLPQVEEPGRLADLIRT"
     gene            624..1541
                     /locus_tag="PKY13_08675"
     CDS             624..1541
                     /locus_tag="PKY13_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT80049.1"
                     /translation="MSDTTAQQRAEGCSEEVRAWADRGAIHQLAGHGVFVVDVPAADP
                     DPALEPLLVLHGFPTSTFDYAGIIDRLAAHRRVIAADFLGFGLSDKPDLHYTMGLQAD
                     LIVALTAELGLTRVALLSHDMGDTVGGELLARNTEGAWPIEVTRRVVTNGSIYIGLAQ
                     LTDGQQFLLALPDERVAEGLGPQPEGLAASLVATLSPTTTLGVADLLPHGELVAALDG
                     GRVLPRTIRYIEERRANERRFTGAIESHPSPLAIVWGDDDPIAVTAMTAELVAARPDA
                     ALTILDGIGHYPMLEAPERFLDAVLPALG"
     gene            complement(1552..1935)
                     /locus_tag="PKY13_08680"
     CDS             complement(1552..1935)
                     /locus_tag="PKY13_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80050.1"
                     /translation="MTLNVLVSDNLVSIRFTGSDRLLSLCRYVDLHGDEITGARLLPR
                     SEAKALLGWRVGGSYWPGAFANGWFTTPGQRRQLQLWDVYRDDEVLVIDTTRPKPSRI
                     VLQHPDRARLVERINAVASAPPSAE"
     assembly_gap    1955..2054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2055..>4039)
                     /locus_tag="PKY13_08685"
     CDS             complement(<2055..>4039)
                     /locus_tag="PKY13_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOT80051.1"
                     /translation="LGELEHINYTDEELESALDDAVARAGAEGERMLVALDAFVVGLN
                     TWLDRNTFPPEARALGLQWRHWTRADVLAVGIVVDDIFGAGGGDELGNANALATLEAR
                     LGTAAGRATFDDLRSADDPAATAHTAAAFPYPQFTGAGVGVSSANTVDPAAVARPDAP
                     VAVARTEAGTPTMSNYIAVAGDHTASGHPILVGGPQSSYFAPQLLFEMELRGGGYDSR
                     GITFPGLGPWVVIGRSRTYAWTATAGGSDLTDQRMELLCEPDGSAPSQLSMHYEFEGR
                     CLPMTRPANDDPWTAWRTVHGIVVGRSTVDGRPVAVARQRMSRFQTAHAAPAFWALNQ
                     GAVTSAADFAPTMSDIPMSFNWLYVNASDIAYFHSGWYPIRARGVDPDLPSWGTGDWE
                     WQGRLDWRRQPQEIDPAKGYAVSWNNKVAPGWREPDNDWDAGIVQRVDTLASRTEALI
                     DRKDGAVTPADVFVAAQDAATVDVRGELVVPEVLAILDGAPAPNPDLAAARDRLSSWV
                     SNGAHRRDRADDGFYDDLAVSLMDALFEPMTRAVFTPGLGSYLGDGVRRPRVIDNPPS
                     QTGGAFAHGWYSAVVHDLRRVRGVESTPGGVPSFCGSGDLAACRALLWQVLDSSRVAV
                     GPFPRLALLERIRFIPYITNGSSVRWMNRPTYQQIIS"
     assembly_gap    4040..4139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4140..4740)
                     /locus_tag="PKY13_08690"
     CDS             complement(<4140..4740)
                     /locus_tag="PKY13_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOT80052.1"
                     /translation="MFRNRAGRHAISSSLLVITALIAGCTGAPPSGEPLPAGGVASNP
                     GGNADPALVALAVLPPGNGNVTGHTAAAIDDQRVMYDAVEDAVADGTLTDANLGQFFT
                     DNHLGAGRIVRVDRPAPGVRVEWDEHGVPHVIGDTAADVGFGAGWAVAESRLVVAELG
                     RLLGRAGTLELGGPDIPTAVARLGELEHINYTDEELESAL"
     gene            4898..6229
                     /locus_tag="PKY13_08695"
     CDS             4898..6229
                     /locus_tag="PKY13_08695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOT80053.1"
                     /translation="MTNLVGYGSERTTEFLAAHDGSLLGEPNGRDAVFEALYHAAGAS
                     ADPVFIVGGRRDRLEIRWANRAFADAVCAEVSDLADTPLEAVLVSSAGALPLNDLRRA
                     EFRATLSPRVGFASPWEAVAVPSVGPAGRCWVVTLRRSGDSLNADQLLRASEERFRAL
                     SERAPIGIITSEVGLRIGYINDFLAELVGLPTEQLLGTGWMQVVDPEHLETVCSCLQE
                     ALAGNPVETPARLITHSGDERWVTIRAVPIHAPDSPAAFLGTIEDVTDRRHFEQLLSW
                     QASHDPLTRLPNRARLIEEIGVATADASDDVAVLFVDLDDFKSVNDTLGHAAGDELLV
                     EVAARLRATTGDSCRVFRFAGDEFVVLARGIHDDEEAAAIGERLRVELAEPMLLESGP
                     LVVTCSVGVARATGDDSVDGLLHNADVAMYEAKRRGKGATAVFGALPSGQG"
     gene            6232..7131
                     /locus_tag="PKY13_08700"
     CDS             6232..7131
                     /locus_tag="PKY13_08700"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOT80054.1"
                     /translation="MALSAPGAPGAPAAAAALAAATPLSAAERDADERREITVELCRA
                     LDSVSVAKPAAMRVLSVVDNPNTNAQMVAQAIELDPTFAAQIMRLGNSAYYGMSGKVG
                     NTGFAVSVIGFAAVRSMAAISATGLSTQSAIPDGFWRHAAAAAAGASVVAPRLGLPAA
                     DCFAAGLLHDVGTAFLHGFDQQGHLALIAEHGTDGPGLAAAEADRYGMGHDAAAAHVL
                     SAWKFPLPFVEAVAWHHHATEGNALADAVRTGDLLALLSEGLGDALVVSELIEAGIPE
                     EEQEELVVATGDRAAEILSTLPI"
     gene            7374..>8811
                     /locus_tag="PKY13_08705"
     CDS             7374..>8811
                     /locus_tag="PKY13_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80055.1"
                     /translation="MIRRPRAANRSTPHRGAGALATLAIGVGVLVPALQPGAGAAPVA
                     DATNATAAASAWLAATAPSGVLTTGGVADWGLTADGVFAHAAGGRGPTAGETATDQLE
                     THLLDYASAPGGTARGAGPIAKLLVVAVVQGRDVTDFGGWNLRSELLALMNTTAGDAN
                     LGRFQSKGVGDTTNGFSQALAVIGLSRTGTVPAEAVSFLLAQQCPSGGFRLFYAGPGC
                     TADAAADVDATAMAVQALAAAKASGATGTETPTANGLDWLEVLQGVDGSFTASGPNGV
                     PNTNTTALSSQALRAAGRANAADNASSWIRGLQLDCDLPALSSEIGAVAYGPTAYDDT
                     VGGGVIDDLYADQFVRATAEAALGLGAPSFATLSGTTASDGPGGFDCDAPTTSSTSST
                     SSTSSTSTTSSTVPGSTTTSTTTSTTVRAATTTTTIAVGLIDAAVGSSSRAVVEGTSQ
                     SAGSRSSSSSRSTGLAATGLGAGRLAVAS"
BASE COUNT         1209 a         3110 c         3008 g         1284 t
ORIGIN      
        1 cactgcacgg tcgtcgccag ttccctcacg ggggcgtacg cgacgaaggc catcgccgat
       61 tcgatccgac cgggcgacac tccagcggac acgccgcgcc tcgtcctcgt cactccgact
      121 ggcctcggcg acgcccagcg ccggcgcggc ggcctcgttg cccgggcggc gtacgccgtc
      181 ggcaggcaca ccccgatcgg agatctcgcc gtcgccgcgc tcacgtcggg cccgagcgtg
      241 cggtggttcc agcgcaacaa gacctactcg aaccccgagg cgcttcacga ggacgaagtc
      301 gtcgagaccc gacgcgccgg tcgtctgccc aacggcaagc acctgcagct cgccttcgtg
      361 accggtcgcc tcgccctccc gctgtcgcgt gccgacgtgc gcgccgcccg accgctcgtg
      421 gtgtgggcgc gtggccaggc cttcagcgag gacagcgaca tggcgttgtg gcaggcggcg
      481 ggggcgaccg tcgtcgccgt cccgagcggg ctgccacagg tcgaggaacc gggccggctg
      541 gccgacctga tccgtacctg aacctgcggt tgcgctgcgg tgggccgcgc cggcgagccg
      601 cgccggggta gcgtcgccgt ccgatgagcg acacgacagc acaacaacgg gccgagggat
      661 gttccgagga ggttcgtgcc tgggccgacc gtggtgcgat ccaccaactc gccggccacg
      721 gcgtgttcgt cgtcgacgtg ccagcggccg accccgatcc ggcgctcgag ccgctgctcg
      781 tcctccacgg gttcccgacg tccacgttcg actacgccgg gatcatcgac cggttggccg
      841 cccaccggcg agtcatcgca gcggacttcc tcggcttcgg gctcagcgac aagcccgacc
      901 tgcactacac gatggggctc caggccgacc tgatcgtggc gctcaccgcc gagctcggcc
      961 tcacccgggt cgcgctcctg tcgcacgaca tgggtgacac cgtcggcggc gaactgcttg
     1021 cccgcaacac cgagggcgcg tggccgatcg aggtcacccg ccgggtggtc accaacggca
     1081 gcatctacat cgggctggcc cagctcaccg acggtcagca gttcctcctg gcgctccccg
     1141 acgaacgcgt cgccgagggc ctgggccccc aacccgaggg gctcgccgcg agtctcgtcg
     1201 ccacgctcag ccccacgacg acgctcggcg tcgccgatct gctgccccac ggtgagctgg
     1261 ttgccgcgct cgacggtggt cgggtgctgc cgcgaacgat ccgctacatc gaggagcgac
     1321 gcgccaacga gcgacgcttc acgggcgcga tcgagtcgca tccgtcaccg ctcgcgatcg
     1381 tgtggggcga cgacgacccg atcgccgtca cggcgatgac cgccgagctg gtggccgccc
     1441 gcccggacgc cgcgctcacc atcctcgacg gcatcggtca ctacccgatg ctcgaggccc
     1501 ccgagcgctt cctcgacgcc gtgctcccag ccctgggctg agccggcgtc gtcactccgc
     1561 cgacggtggc gcgctggcga cggcgttgat gcgttcgacg agccgagccc gatcggggtg
     1621 ctgcagcacg atgcggctcg gcttcgggcg ggtcgtgtcg atcaccagca cttcgtcgtc
     1681 gcggtacacg tcccacaact gcagctggcg gcgctggccc ggcgtggtga accatccgtt
     1741 ggcgaacgcg cccggccagt agctgccccc gacccgccag ccgagcagtg ccttggcctc
     1801 gctgcgcggc aacaaccgcg ccccggtgat ctcgtcgccg tggaggtcga cgtagcgaca
     1861 cagcgagagc agccgatcgg agcccgtgaa gcggatcgac accaggttgt cggagacgag
     1921 gacgttcagc gtcacggcag agcatcgtac cggcnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnaagctg atgatctgct ggtaggtggg ccggttcatc caccgcaccg
     2101 aactgccgtt ggtgatgtag gggatgaacc ggatccgttc gagcagcgca agccgcggga
     2161 atgggcccac cgcgacccgt gacgagtcga gcacctgcca cagcagggcc cggcacgcgg
     2221 cgaggtcgcc gctcccgcag aacgacggca caccacccgg ggtcgactcg acgccacgta
     2281 cccgacgcag gtcgtgcacc acggcggagt accacccgtg cgcgaatgcg ccaccggtct
     2341 gcgacggcgg gttgtcgatg acgcggggtc gacgtacgcc gtcacccagg tacgaaccga
     2401 ggccgggcgt gaacacggcg cgcgtcatcg gctcgaacag ggcgtccatc aacgagacgg
     2461 cgaggtcgtc gtagaacccg tcgtcggcgc ggtcccggcg atgggcaccg ttcgacaccc
     2521 agctcgacag ccggtcgcgc gccgctgcca gatcggggtt cggagcgggg gcaccgtcga
     2581 ggatcgcgag gacctccggg accaccagtt cgccgcgaac gtcgacggtc gccgcgtcct
     2641 gggcggcgac gaacacatcg gcgggtgtga cggcaccgtc ctttcggtcg atgagcgcct
     2701 cggtgcggga cgcgagggtg tcgacccgtt gcacgattcc ggcgtcccag tcgttgtccg
     2761 gctcgcgcca gcccggcgcc accttgttgt tccacgacac cgcgtagccc ttggcggggt
     2821 cgatctcctg gggctgtcgg cgccagtcga gccgaccctg ccactcccag tccccggtgc
     2881 cccacgaggg caggtccggg tcgacgcccc gggcccgaat cgggtaccag ccgctgtgga
     2941 agtacgcgat gtcggacgcg ttcacgtaga gccagttgaa gctcatcggg atgtcgctca
     3001 tcgtcggggc gaagtcggcg gccgacgtga ccgctccctg gttcagtgcc cagaacgccg
     3061 gggcggcgtg cgcggtctgg aaccgcgaca tccgttggcg ggcgacggcg acggggcgcc
     3121 cgtcgacggt cgaccggccg acgacgatcc cgtgcaccgt gcgccacgcc gtccaggggt
     3181 cgtcgttggc cgggcgggtc atcgggaggc accggccttc gaactcgtag tgcatcgaca
     3241 gctgcgacgg cgccgaaccg tcgggctcgc acaggagctc catgcgctgg tcggtgaggt
     3301 ccgatccgcc ggcggtggcg gtccaggcgt aggtgcggga ccggccgatc acgacccacg
     3361 gaccgaggcc ggggaacgtg atgccccgcg agtcgtagcc gccgccgcgc aactccatct
     3421 cgaacagcaa ctggggagcg aagtacgacg attgtggccc gcccacgagg atggggtggc
     3481 ccgatgccgt gtggtcgccg gcgacggcga tgtagttcga catcgtgggt gtgccggcct
     3541 cggtgcgggc gacggcgacc ggtgcgtcgg gtcgtgccac ggcggcgggg tcgaccgtgt
     3601 tcgccgacga cacgcccacc cccgccccgg tgaactgcgg gtaggggaac gctgcggccg
     3661 tgtgggcggt ggctgcgggg tcgtcggcgg agcggaggtc gtcgaacgtg gcgcgaccgg
     3721 cggcggtgcc cagccgcgcc tcgagggtgg ccagtgcgtt ggcgttgccc agttcgtcgc
     3781 ccccgccggc accgaagatg tcgtcgacga cgatgccgac ggccaggacg tcggctcggg
     3841 tccaatgacg ccactgcagg cccagtgccc gagcttcggg cgggaacgtg ttgcggtcga
     3901 gccaggtgtt cagtccgacc acgaaggcgt cgagcgcgac gagcatccgc tcgccctcag
     3961 cgcccgctcg ggccacggcg tcgtcgaggg ccgactcgag ttcctcgtcg gtgtagttga
     4021 tgtgttcgag ctcacccagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4141 gagggccgac tcgagttcct cgtcggtgta gttgatgtgt tcgagctcac ccaggcgggc
     4201 gaccgcagtg gggatgtcgg ggcccccgag ttcgagggtg ccggcccgcc ccaggaggcg
     4261 gccgagttcg gcgacgacga gccgcgactc ggcgacggcc caccccgccc cgaacccgac
     4321 gtcggccgca gtgtcgccga tcacatgggg aaccccgtgc tcgtcccatt cgacccggac
     4381 ccccggggcc ggccggtcga cccgcacgat ccgccccgca ccgaggtggt tgtcggtgaa
     4441 gaactgcccc aggttcgcgt cggtcagggt gccgtcggcg acggcgtcct cgacagcgtc
     4501 gtacatcacc cgttgatcgt cgatcgccgc ggccgtgtgc ccggtgacgt tgccgttgcc
     4561 gggcggcagc acggccagcg cgaccaacgc ggggtcggcg ttgcctccag gattggacgc
     4621 aactccgccc gccggaaggg gctcccccga tggaggtgcc ccggtgcatc cggcgatcag
     4681 ggccgtgatc acgaggagcg atgacgagat cgcgtggcgt ccggctcggt ttctgaacat
     4741 tctcgctccc tgtcgtgcgc tttcacgcca cgccgaaagg gtactgattc gccggacctc
     4801 gagtcgcacc gagcaacttc atgttcaagt ccgggccgtt cgttccgata tttgacagga
     4861 aggttcaccc aacccacgtg tgcgaagcga gagacagatg accaacctgg tgggatacgg
     4921 gagcgagcgg acgacagagt tcctcgccgc ccatgacggg tcgctgctcg gcgaaccgaa
     4981 cggcagagac gccgtgttcg aggcgctgta tcacgccgcc ggcgcgtcgg ccgatcccgt
     5041 gttcatcgtg gggggtcgcc gcgaccgcct cgagatccga tgggcgaacc gtgcgttcgc
     5101 cgacgccgtg tgcgccgagg tgtcggatct cgccgacacc ccgctcgagg cggtgctcgt
     5161 gtcctcggcc ggagcgctgc cgctcaatga cctccgcagg gccgagttcc gagcgacgct
     5221 gtcgccgcgg gtcgggttcg cctcaccctg ggaagccgtt gcggtcccga gcgtcggccc
     5281 cgccggccgc tgctgggtcg tcaccttgcg tcgatcaggc gacagcctga acgccgacca
     5341 gctcctccgc gccagcgagg agcgcttccg ggcgctgtcc gaacgtgcgc cgatcggcat
     5401 catcacgtcc gaggtcggcc tgcggatcgg gtacatcaac gacttcctcg ccgagctcgt
     5461 ggggcttccc accgagcagc tgctcggcac cggctggatg caggtggtcg accccgagca
     5521 cctcgagacg gtgtgctcgt gcctccagga agcgcttgcg gggaaccccg tcgagacccc
     5581 cgcccgactc atcacccaca gcggtgacga acggtgggtc acgatccgcg ccgtcccgat
     5641 ccacgcgccg gattcaccgg cagcgttcct cgggacgatc gaggacgtca ccgaccgtcg
     5701 ccatttcgaa cagctgctgt cgtggcaggc cagtcacgac ccgctgacgc gtctgcccaa
     5761 ccgcgcccgg ctgatcgagg agatcggcgt tgcgacggca gatgccagcg acgatgtcgc
     5821 cgtcctgttc gtcgatctcg acgacttcaa gtcggtcaac gacacgctcg ggcacgccgc
     5881 cggcgacgag ctgctggtcg aggtcgctgc ccggctccgg gcgacgaccg gcgacagctg
     5941 ccgtgtcttc cgcttcgccg gcgacgagtt cgtggtgctc gcccgcggga tccacgacga
     6001 cgaggaggcg gccgccatcg gcgagcggct ccgcgtcgag ctcgccgagc cgatgctgct
     6061 cgagagtggg ccgctggtcg tgacctgctc ggtgggcgtg gcgagggcga cgggcgacga
     6121 ctccgtcgac ggtctgctgc acaacgccga cgtcgccatg tacgaggcca agcgccgcgg
     6181 caagggcgca acggcggtct tcggcgctct gccgtccgga cagggttgat catggcgctc
     6241 agtgcccccg gagcccccgg cgcaccggcg gcggccgccg ccttggccgc cgcgactccc
     6301 ttgtccgctg cggagcgtga cgccgacgag cgtcgggaga tcaccgtcga gctgtgccgg
     6361 gccctcgact cggtgtcggt cgccaagccg gccgccatgc gggtgctgtc ggtcgtcgac
     6421 aaccccaaca ccaacgccca gatggtcgcc caggcgatcg agctcgaccc gacgttcgcc
     6481 gcgcagatca tgcgtctcgg gaactcggcg tactacggga tgagcggcaa ggtcggcaac
     6541 accggcttcg ccgtctcggt catcgggttc gccgccgtcc gctccatggc ggcgatcagc
     6601 gccacggggc tctcgaccca gagcgccatt cccgacggct tctggcgtca cgccgctgct
     6661 gctgccgccg gcgcctccgt cgtcgcgccc cgcctgggcc ttccggccgc cgactgtttc
     6721 gccgccggcc tgctccacga cgtcgggacc gcgttcctgc acggtttcga ccagcagggg
     6781 catcttgccc tgatcgccga gcacggcacc gacgggcccg ggctcgccgc cgctgaggcc
     6841 gaccgctacg gaatgggcca cgacgccgct gccgcgcacg tgctgtcggc gtggaagttc
     6901 ccgctgccgt tcgtcgaggc ggtcgcctgg caccatcacg ccacggaggg caacgccctc
     6961 gccgatgccg tgcgcaccgg cgatctgctc gccctcctgt cggaggggct cggcgatgcc
     7021 ctcgtcgtga gcgagctgat cgaggccggc atccccgagg aggagcagga agagctcgtc
     7081 gtcgccaccg gtgaccgcgc cgccgagatc ctctcgacgc ttccgatcta gctgcttggg
     7141 tccacgcgcg gcacgtcctg gtggaccgtc gaggtgttga ggatggtgct gtcgcggcct
     7201 gattcgggcg cgccggcgca ccatccttcg gaggccggtg agttcgctgg tgagttcgcc
     7261 agcgcgggcc ttcgggatcc ccccaactac tgacctcatg atccggacat ctacgatgcc
     7321 ggtcacttgg cgtgcgggag tggcgcgccg gtgaccgaat cgaggacgtc cacatgatcc
     7381 gcaggccccg agccgccaac cgcagtacgc ctcatcgagg tgccggagcg ctggccaccc
     7441 tcgcgatcgg tgtgggggtc ctggtcccgg cgctgcagcc gggtgcgggg gcggccccgg
     7501 tggccgacgc caccaatgcc accgctgccg cgtcggcgtg gttggccgcc acggcaccgt
     7561 cgggggtcct gacgacgggg ggcgtcgccg attggggtct cactgcggac ggcgtgttcg
     7621 cccacgctgc tggtggtcgc ggccccaccg ccggcgagac ggccaccgac cagctcgaaa
     7681 cccatctcct cgactacgcg tcggcgccgg gcggtacggc tcgtggcgcc gggccgatcg
     7741 cgaagctgct ggtcgtcgcc gtcgtccaag gccgcgacgt caccgacttc ggcggttgga
     7801 acctccgctc cgaactcctg gccctcatga acaccaccgc cggcgacgcc aacctcgggc
     7861 ggttccagag caagggcgtg ggcgacacga ccaatggctt ctcccaggcc ctggccgtga
     7921 tcgggctgtc gcgtaccggc accgttcctg ccgaggcagt gtcgttcctc ctcgcacaac
     7981 agtgcccgag cggcgggttc cggctcttct atgcgggccc cggctgcacc gccgacgctg
     8041 ccgccgacgt cgacgccacc gcaatggcgg tgcaggcgct cgctgcggcc aaggcgtcgg
     8101 gcgcaaccgg caccgagaca ccgacggcga acggcctcga ctggctggag gtcctccaag
     8161 gggtcgacgg ttcgttcact gcgtcgggtc ccaacggggt gcccaacacc aacacgacgg
     8221 cgctgtcgtc gcaggcgttg cgtgccgccg gccgcgccaa cgccgccgac aacgcgtcct
     8281 cgtggatccg cggcctccag ctcgactgcg atcttccagc cctctcctcg gagatcggcg
     8341 ccgtcgccta cggccccacc gcctacgacg acaccgtcgg cgggggtgtc atcgacgatc
     8401 tctacgccga tcagttcgtg cgagccaccg ccgaggcggc gctcggcttg ggcgctccgt
     8461 cgttcgccac cttgagcgga accacggctt cggacggtcc gggcggcttc gactgcgacg
     8521 ctccgacgac ctcgagtacg tcgtccacct cgagtacgtc gtccacttcg accacgtcct
     8581 ccaccgtccc gggttcgacc acaacgtcga cgacaacgtc gacgacggtc cgggccgcga
     8641 ccacgaccac cacgatcgcc gtcggtctga tcgacgccgc ggtcggctca tcgtcgcgtg
     8701 ccgtggtcga ggggacgagt cagtcggcgg ggtcgaggtc gagttcgagc tcgcgctcca
     8761 cgggcctggc cgcgacgggg ctcggtgccg gccgcctggc ggttgcctcc g
//
LOCUS       DAOSOA010000114         8762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_7993_length_8762_cov_7.429080, whole genome shotgun sequence.
ACCESSION   DAOSOA010000114 DAOSOA010000000
VERSION     DAOSOA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7993_length_8762_cov_7.429080"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1341
                     /locus_tag="PKY13_08710"
     CDS             <1..>1341
                     /locus_tag="PKY13_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT80056.1"
                     /translation="IDDQHRQFLVALATAPAERRVLTIARGDLRGSRRSLPSRWLLDT
                     ASALAGTVVHSTEFDRLPPSVVTVVPSHATGVVTAPVHASLADRDLAAMSRLSAQGVD
                     VTDHPTMANVHRGVWAQILRRSDRFTEWDGNLAHQRVRSPGADPWSPSRLQAWASCGY
                     RYFLGYQLGLSERDEPERVLDLGPLDRGSGVHEVLERFLTEVIEHGPPAPDERWLPHH
                     RERLREIAAGVFDEYERRGRTGRPVHWALTRADLLDTLDAFLVADDTHRAATGSRPDR
                     VEYPFGLKGNEPATIRLANGRSLAFRGMADRIDRSDTGRVLVSDYKTGKGAQYKGLGA
                     DGDDPVRGGTLLQLGIYAEAALQQLDATEADAHYWMINGDVAFARFGYRWTPERRQRL
                     IDVVTTIVDGIDAGVFPAVPGEWNNFRNTHENCTYCEFDRVCSRDRGEHAEAKVG"
     assembly_gap    1342..1441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1442..4846
                     /locus_tag="PKY13_08715"
     CDS             <1442..4846
                     /locus_tag="PKY13_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT80057.1"
                     /translation="VERIANLVVDRGVRLAEIAAITFTEAAAAELQSRIRVQFERRAE
                     ASTDPAERERCRVAIAEADLAAISTLHGFASRLLGEFSTEAHLPPRVRVLDEVSSQLA
                     HEARWERFVDGLYDDPANEAVLVRAAMVGVPLEPRYQGHATLKDVAAELAQNWDRIGE
                     LAAVDHPPLGPAVTEPFDRAAEAVMALPSACTDPDDKFFVHLVTEVIPEMSAIVGIAD
                     DERKLQALSGAKWSKGRGGTKAAWGGDVASCKALIDEVNEAAAAVRDAAADEVLQRLL
                     TLAAREVLAAAAARRDEGGLEFHDLLVLARELLRTSPTARTTLHERYTHLLLDEFQDT
                     DPIQIELAMLIASSIDAGDGDPGPGFEPPAAAPAHWSDLRVAEGRLFFVGDPKQSIYR
                     FRRADIDLFLRARDRFGIDGASVKLTTNFRTVAPVVEWVNALFEDAMPEEVPGAQPRY
                     EPLVPFRRPEAGNDHRPLLLGGPHPNPKVRAAELREAEAADVARTVRDVQHHPERWPV
                     HDEHSGEWRPATLADVTILVPTRTSLPFLRAALEASAIPYRLATGTLVYDSQDVADVL
                     AAVRAVDDPSDELSLVAALRSPLYACSDVDLFTFHDAGGRWNLRVKPPESVAADHPVR
                     LALAHLRSLWVDRWWLTPATLVDRIVRERQADLLAFGDPRPREVWRRLRFLVDQARTF
                     EEAGGGGLRAFVEWAALHGADGARVHEPLLPETDDDAVRILTIHGSKGLEFPITILSG
                     MTTQPGNARRGVSVVWGDDGPPEVRLNAKAATANHEPRADIEIEMDAYEKLRLLYVAT
                     TRARDHLVVSCHHKVSDKPVDTYARRVWTFFHDRPDLVRPEAPDQGSWREVEMFPGVG
                     GVPATPEAVVPAAPLTPSPPAAGDPDRDAWIAEREALLAPQRRARFVSATAVATSALA
                     PPVNPSATGSAATAPAAGDPADPAWADDDPVTEPPTADDAVGAVPTRRRGRAGTAIGR
                     AVHATLQVLDLADPHDIELQAHRQCDVEAIPEEVRTVTALVRSALDSTTVREAAVSTH
                     HKELYVAAPVGARVIEGFVDLLIETPDGLIVVDYKTDAAASDAAIDAALARYELQGAS
                     YAVALEAVTGIPVIDCRFVFCKARGAVERSVRDLPAAMARVRATVA"
     gene            complement(4859..6031)
                     /locus_tag="PKY13_08720"
     CDS             complement(4859..6031)
                     /locus_tag="PKY13_08720"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOT80058.1"
                     /translation="MRTIRRSGPHGWFVSFAATAVLLLVALTACGSGERSAASGRDDP
                     AGTAPAAAESGRPSSTVDTDTPTVAPGPAVPSRGCAAGPTAAVQLERRTVPVAELSRW
                     YLLTVPEASDPQVPFPLVLDFHGLTEGADVHAQLTGMGPYGASHGFVAVIPQGSGDLA
                     RWAAGPGGSAGEPNIDVDFVTAMLDQVEDSVCIDTSRVYATGMSNGAMLTSMLACRLA
                     GRLAAVAPISGATTFDSCEPVATVPMMTIHGTADPILLFNGGVGDLVKALSGSLADVG
                     SAPSSGPSTTTPAPTTTAAVDLDGPGYPANVAAWAQRNGCGPTPSDEAVSATVTRRTY
                     DCPAGADVEMYVVEGGGHAWPGSELSRALEGLVGTTTFDIDATDLVWAFFQRFRRG"
     gene            6053..8041
                     /locus_tag="PKY13_08725"
     CDS             6053..8041
                     /locus_tag="PKY13_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT80059.1"
                     /translation="MQSQITSGGVVVADAVLGVHLGPALPTLSMTVGGNRHDWRATSL
                     VDATHGWLARVGDTGVTVELMANPTPDGMAVGARIVNHSGTDVTLHRSSVIATEHFGL
                     SGARADWRIYRHSYQSWAGTWTIGVGEHDRDVPTRFGRLGVTDGRHRAARHRGHVRSD
                     SLSVIAGNGSGALGAGFTTLSDAFGFVEIDAPSGRVRGFDVWVDLDDTVLAAGAATPW
                     YEVRIVIAADGNTALRSLIAAAGAAAGARGVDRPHPTGWCSWYYYFTKVTEADVLANL
                     RVLESDGRGGPRFGCEYVMVDDGHQTAIGDWLSTNDKFPRGMAAVAADIGAAGFDAGI
                     WWAPFIVSSRSRIAAEHPEWLVRNQRGRPVLGLFNPGWGLHPMWVLDTTHPDALAHIT
                     EVARVISRDWGYAIQKIDFLFAASHPGVRHDPTATRAQALRRGLDAVRAGAGDDAFVL
                     GCGSPLGPAVGVVDAMRIGADVTPSWTTTLARTVGRNRHALSTANALLNTMTRSAFDG
                     VWFLNDPDCLMVRDTDTKLTEAEVHTMCTVFGLTDGMVVVSDRLDLLPPERQDLVART
                     LALTGGEVTVVDLFERALPRVLVSRHADRVDVAVVNLGDGPCSGRVDLAAAGIDDTWV
                     SGELREHWTGRVVSSGPDHVDLGEVAPHGCGLVQLRRR"
     gene            8082..8657
                     /locus_tag="PKY13_08730"
     CDS             8082..8657
                     /locus_tag="PKY13_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT80060.1"
                     /translation="MGYRHQRQDLLDAAVELALSDGLSTLTFGGVAEILGINDRTVVY
                     YFPSKRNLVTDVVLALDIRLRALVGDAVGPEPLPVEALLQRVWPVLASTAADPLTAAL
                     LELSGLAAAGHQPYADLAPGLIAGWVDRLVRQIVPSGPTSARPEASAALAQLSGLVTL
                     RHLSGARAANSAARHLGVIATAPVGRTGRRA"
BASE COUNT         1241 a         3219 c         2957 g         1245 t
ORIGIN      
        1 catcgacgac cagcaccgcc agttcctcgt tgccctcgcc acggcgccgg ccgagcgccg
       61 ggtcctcacc atcgcccggg gcgatctgcg cggcagccga cgttcgctcc cctcacgatg
      121 gctcctcgac accgcctccg cgctggccgg caccgtcgtg cacagcaccg agttcgaccg
      181 tctcccgccg tcggtcgtga ccgttgtgcc gtcccacgcc accggtgtcg tgacggcacc
      241 ggtccacgcc tcgctcgccg atcgagacct ggccgccatg agtcggctct cggcgcaggg
      301 cgtcgacgtg accgaccacc ccaccatggc caacgtccac cgcggcgtgt gggcgcagat
      361 cctgcggcgg tccgaccggt tcaccgagtg ggacggcaac ctcgcgcacc agcgcgtgcg
      421 ctctccgggc gccgatccct ggtcgcccag ccggctgcag gcgtgggcgt cgtgtggcta
      481 ccgctacttc ctcggctacc agctcgggct cagcgaacga gacgaacccg aacgggtgct
      541 cgacctcggc ccgctcgatc gcggctcggg tgtccacgag gtgctcgagc ggttcctcac
      601 cgaggtcatc gaacacgggc cacctgctcc cgacgagcgc tggttgccgc accaccgcga
      661 gcgcctccgt gagatcgcgg cgggggtgtt cgacgagtac gagcgccgcg gccgcaccgg
      721 ccgtccggtg cactgggcgc tcacccgggc cgatctgctc gacacgctcg atgcctttct
      781 cgtcgccgac gacacccacc gcgccgccac cggctcgcgg cccgatcgcg tcgagtatcc
      841 gttcgggctg aagggcaacg agccggcgac catccgcctc gccaacggcc gcagcctggc
      901 gttccgcggc atggccgatc gcatcgaccg gtccgacacg ggccgggtgc tcgtgagcga
      961 ctacaagacc ggcaagggag cccagtacaa gggtctgggc gccgacggcg acgatccggt
     1021 gcggggcggc accctgctcc agctcggcat ctacgccgaa gcggccctgc aacaactcga
     1081 cgccaccgag gccgacgccc actactggat gatcaacggc gacgtcgcct tcgcacggtt
     1141 cgggtaccgg tggacgccgg aacgacgcca gcgcctgatc gacgtcgtga ccacgatcgt
     1201 cgacggcatc gacgccggcg tcttccccgc cgtgcccggt gagtggaaca acttccgcaa
     1261 cacccacgag aactgcacct actgcgagtt cgaccgcgtg tgctcgcgcg accggggcga
     1321 acacgccgag gccaaggtcg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 ngtcgaacgg atcgccaacc tcgtcgtcga ccggggtgtc cgactcgccg agatcgccgc
     1501 catcacgttc accgaggccg cggcggccga gctgcagagc cgcatccggg tgcagttcga
     1561 acgccgggcc gaggcctcca ccgacccggc cgaacgcgaa cggtgtcgag tcgcgatcgc
     1621 cgaggccgac ctcgccgcca tctcgaccct ccacgggttc gcgagccgac tgttgggcga
     1681 gttctcgacc gaggcgcacc tccccccgag ggtgcgggtg ctcgacgagg tgtcgtcgca
     1741 gttggcacac gaggcgcggt gggagcgctt cgtcgacggc ctctacgacg accccgcgaa
     1801 cgaggccgta ctcgtgcggg ccgccatggt gggggttcct ctcgagccgc gctaccaggg
     1861 ccacgccacc ctgaaggacg tcgctgcaga gctcgcacag aactgggacc gcatcggcga
     1921 actcgccgcc gtcgaccacc ctccgctcgg tccggccgtc accgaaccgt tcgaccgggc
     1981 ggccgaggcg gtgatggcac tgccgtccgc gtgcaccgat cccgacgaca agttcttcgt
     2041 gcacctcgtc accgaggtca tcccggagat gtcggcgatc gtcggcatcg ccgacgacga
     2101 acgcaagctc caggccctct cgggggcgaa gtggagcaag ggtcgcggcg gcaccaaggc
     2161 tgcatggggt ggcgacgtcg cctcctgcaa ggccctgatc gacgaggtca acgaggcggc
     2221 ggccgcggtc cgcgacgctg cggccgacga ggtgctccaa cgtctcctca cactcgccgc
     2281 ccgtgaggtg ctggccgccg ccgcggcccg gcgcgacgag ggcggcctcg agttccacga
     2341 cctgctggtg ctggcccgcg agctgctgcg caccagcccg accgctcgga cgaccctgca
     2401 cgagcgctac acccacctgc tcctcgacga gttccaggac accgacccga tccagatcga
     2461 gctggccatg ctcatcgcca gctcgatcga cgcgggcgac ggcgaccccg ggccggggtt
     2521 cgagccgccg gccgcagcgc cggcccactg gtccgacctc cgggtcgccg aagggcgcct
     2581 gttcttcgtc ggcgatccca agcagtcgat ctaccggttc cgccgcgccg acatcgacct
     2641 gttcctccgg gcgcgcgacc gcttcggaat cgatggcgca tcggtgaagc tcacgaccaa
     2701 cttccggacc gtggcgccgg tcgtcgagtg ggtcaacgcg ctgttcgagg atgcgatgcc
     2761 cgaggaggtc cccggcgccc agccacgcta cgagcccctc gtgccgttcc gccggccgga
     2821 ggccggcaac gatcaccgtc ccctgctcct cggcggtccg caccccaacc ccaaggtccg
     2881 cgccgccgag ctccgtgagg ccgaggccgc cgacgtcgcc agaaccgtgc gcgacgtcca
     2941 acaccatccc gagcggtggc ccgtgcacga cgagcattcg ggcgagtggc ggcccgccac
     3001 gttggccgac gtcacgatcc tcgtgcccac gcgcacctca ctccccttcc tccgcgccgc
     3061 cctcgaggcc tcggccatcc cgtatcgcct cgccaccggc acgctggtgt acgactccca
     3121 ggacgtcgcc gacgtgctcg ccgcggtccg agcggtcgac gacccgagtg acgagctcag
     3181 cctggtcgca gcgctccgct cgccgctgta cgcgtgctcc gacgtcgatc tcttcacgtt
     3241 ccacgacgcc ggcgggcggt ggaacctccg cgtgaaaccg cccgagagcg ttgcggccga
     3301 tcatccggtg cgcctggcgc tcgcccatct gcggtcgctc tgggtcgatc ggtggtggct
     3361 cacgccggcg acactcgtcg accgcatcgt tcgcgagcgc caggccgatc tcctggcctt
     3421 cggcgatcct cgtccccgcg aggtctggcg gcgcctgcgg ttcctcgtcg atcaggcgcg
     3481 cacgttcgag gaggccggcg gcggtgggct ccgagccttc gtcgagtggg cggcgctcca
     3541 tggcgccgac ggtgcgcggg tgcacgagcc gctgctgccg gagaccgacg acgacgctgt
     3601 gcggatcctc acgatccacg ggtcgaaggg gctcgagttc cccatcacca tcctgtcggg
     3661 catgaccacc caaccgggaa acgcccgtcg gggggtcagc gtcgtctggg gcgacgacgg
     3721 cccgcccgaa gtgcgcctca acgccaaggc cgcgaccgcc aaccacgaac cgcgcgccga
     3781 catcgagatc gagatggacg cctacgagaa gcttcgtctc ctctacgtcg cgaccacccg
     3841 ggcgcgagac cacctcgtgg tgtcgtgcca ccacaaggtg tccgacaagc cggtcgacac
     3901 gtacgcacga cgggtgtgga cgttcttcca cgaccgtccc gacctcgtcc gtcccgaagc
     3961 acccgaccag gggtcgtggc gggaggtcga gatgttcccg ggcgtcggtg gagtcccggc
     4021 cacgcccgag gcggtcgtcc cggcggcccc gctcacgccg tcacccccgg cggccggcga
     4081 ccccgatcgc gacgcctgga tcgccgagcg cgaggcgttg ctggcgccgc aacgccgggc
     4141 acggttcgtc tcggcgaccg ccgtcgcgac cagcgccctc gcgccaccgg tcaacccttc
     4201 agcgaccggc tcggcagcca cggccccggc ggccggcgat ccggccgacc cggcgtgggc
     4261 cgacgacgac cccgtcaccg agccgccgac cgccgatgac gccgtgggtg cagtgcccac
     4321 ccgtcgccgc gggcgcgccg gcaccgcgat cggtcgggcc gtccacgcga cgttgcaggt
     4381 gctcgatctc gccgaccccc acgacatcga gctgcaggcg caccgccagt gcgacgtgga
     4441 ggccatcccc gaagaggtgc gtaccgtcac cgcactggtg cgttccgcgc tcgactccac
     4501 gaccgtgcgc gaggccgcgg tgtcgacaca ccacaaggag ctgtacgtcg ctgccccggt
     4561 cggggcgcgg gtgatcgaag gattcgtcga tctactcatc gagactcccg acgggctgat
     4621 cgtcgtcgac tacaagacgg acgctgcggc gtccgacgcc gcgatcgatg ccgccctcgc
     4681 ccgctacgaa ctccagggtg cgtcctacgc cgtcgcgctc gaagcggtca ccggcatccc
     4741 cgtcatcgac tgccggttcg tgttctgcaa ggcccgcggc gcggtcgagc ggtcggtccg
     4801 agacctgccc gcagcgatgg ctcgggtccg cgccaccgtg gcctgatccc gagccggctc
     4861 agccccgccg gaaccgctgg aagaacgccc acacgaggtc ggtcgcgtcg atgtcgaagg
     4921 tggtcgtgcc gacgaggcct tcgagcgccc ggctgagttc gctgccgggc caggcatggc
     4981 cgccgccctc gacgacgtac atctcgacgt cggccccggc gggacagtcg taggtgcgac
     5041 gcgtcacggt ggcagagacg gcctcgtccg acggggtcgg gccgcacccg ttgcgctgcg
     5101 cccacgcggc aacgttggcg ggatagccgg ggccgtcgag atcgaccgca gcggtcgttg
     5161 tcggtgccgg cgtggtggtg gacggccccg acgatggggc cgacccgacg tccgcgaggg
     5221 agccggagag ggccttcacg agatcaccga caccgccgtt gaacaggagg atcgggtcgg
     5281 ccgtgccgtg aatggtcatc atcggcacgg tggccaccgg ctcgcacgag tcgaacgtcg
     5341 ttgcgcccga gatcggtgcg acggcagcga gacggccggc cagccggcac gccagcatcg
     5401 acgtgagcat ggcgccgttg gacatgccgg tcgcgtacac gcgggaggta tcgatgcaga
     5461 cggaatcctc gacctggtcg agcatcgcgg tgacgaagtc gacgtcgatg ttgggctcgc
     5521 ccgccgatcc gccggggccg gcggcccacc gggcgagatc tccgctgccc tgcggaatga
     5581 ccgcgacgaa gccgtgggac gcaccgtacg gccccatgcc ggtcagctgc gcgtgcacgt
     5641 ccgcgccttc ggtgaggccg tggaagtcga gcacgagtgg gaacggcacc tgcgggtcgc
     5701 tcgcctcggg gaccgtgagg aggtaccaac gggacagctc ggcgaccggc acggttcgcc
     5761 gttcgagctg gaccgcagcg gtcggaccgg cggcgcagcc tcgggatggc accgcgggac
     5821 cgggggcgac ggtcggcgtg tcggtgtcga cggtcgacga aggtcggccc gactcggccg
     5881 ctgcgggcgc tgtccctgcc gggtcatcgc ggcccgacgc tgcggatcgt tcccccgaac
     5941 cacaggcagt gagcgccacc agcaggagca ccgctgtcgc tgcgaacgag acgaaccacc
     6001 cgtgagggcc cgatcgtcgg atggtgcgca tctcgccgac catactggcg ccgtgcagag
     6061 ccagatcacc tccggcgggg tcgtcgtcgc cgatgccgtc ctcggcgtcc acctcggtcc
     6121 ggcactgccg accctctcga tgaccgtcgg tggcaaccgt catgactggc gtgcgacctc
     6181 gctcgtcgac gccacccacg gatggctcgc ccgggtgggc gacaccggtg tgaccgtgga
     6241 gctcatggcg aatcccacgc ccgacggaat ggcggtcggg gcgcggatcg tcaaccacag
     6301 cggcaccgac gtcacgctcc accgcagttc ggtcatcgcg accgagcact tcgggctcag
     6361 cggcgcccgt gccgactggc ggatctaccg gcacagctac cagtcgtggg cggggacctg
     6421 gacgatcggc gtcggcgaac acgaccgcga cgtcccgacc cggttcggtc gcctgggggt
     6481 caccgacggc cggcaccgcg ccgcccgcca tcggggccac gtccgctccg attcgttgtc
     6541 ggtcatcgcc ggcaacggga gcggagccct cggcgccggg ttcacgacgc tctcggacgc
     6601 attcggtttc gtcgagatcg acgctcccag cggccgggtc cgagggttcg acgtgtgggt
     6661 cgatctcgac gacacggtcc tcgccgcagg agcggcgaca ccctggtacg aggtccgaat
     6721 cgtgattgcc gccgacggca acaccgcact gcgctcgctg attgccgccg ctggtgctgc
     6781 ggccggcgcc cgtggcgtgg accgccccca tccgaccgga tggtgctcct ggtactacta
     6841 cttcaccaag gtgaccgagg ccgacgtgct ggccaatctc cgggtgctcg agtccgatgg
     6901 ccgcggcggg ccccgcttcg gctgcgagta cgtgatggtg gacgacgggc accagacggc
     6961 gatcggcgat tggctgtcga cgaacgacaa gttcccccgg gggatggcgg ccgtcgccgc
     7021 ggacatcggc gccgcgggct tcgacgccgg gatctggtgg gctcccttca tcgtgtcgtc
     7081 ccgctcacgg atcgcggcag aacaccccga gtggttggtt cgcaaccaac gtggcagacc
     7141 ggtgctcgga ctgttcaacc ccggatgggg cctccacccg atgtgggtgc tcgacaccac
     7201 ccaccccgat gcccttgccc acatcaccga ggtggcccgc gtgatcagcc gcgactgggg
     7261 ctacgcgatc cagaagatcg acttcctctt cgcagcgtcg caccccggag tgcgtcacga
     7321 cccgaccgcg actcgggccc aggccctgcg tcgcgggctc gacgccgtgc gcgccggagc
     7381 aggcgacgac gcgttcgtgc tcggttgcgg ttctcccctc gggccggccg tcggagtggt
     7441 cgacgccatg cggatcggcg ccgacgtgac cccgagctgg acgacgacgc tggcaaggac
     7501 agtgggccgg aaccgccacg cgctgtcgac ggccaacgca ctgctcaaca cgatgacccg
     7561 atcggcgttc gacggggtct ggttcctcaa cgatcccgac tgtctgatgg tgcgcgacac
     7621 cgacacgaag ctgaccgagg ccgaggtgca cacgatgtgc acggtgttcg gcctgaccga
     7681 tggcatggtc gtcgtgtcgg accgactcga cctcctgccg ccggaacgtc aggatctcgt
     7741 ggcgcgcacc ctggccctca cgggcggcga ggtcacggtg gtcgacctct tcgaacgggc
     7801 gttgccgcgg gtgctcgtgt cacggcacgc cgatcgtgtg gacgtcgccg tcgtgaacct
     7861 cggcgacggg ccgtgctccg gccgcgtcga cctcgctgcg gccggcatcg acgacacctg
     7921 ggtgtcgggc gagctgcgcg aacactggac cggtcgcgtc gtgtcctcgg gcccggacca
     7981 tgtcgacctc ggcgaggtgg ccccgcacgg gtgcggactg gtgcagctcc ggcgccgctg
     8041 aggcgccgac cgaaccgtag cggtcgctac gatcggtgct cgtgggctac cggcaccaac
     8101 gtcaggacct gttggatgcg gcggtcgagc tggcgctgag cgacggcctg agcacactca
     8161 ccttcggcgg agtcgccgag atcctcggga tcaacgaccg aaccgtcgtc tactacttcc
     8221 cctcgaaacg caacctcgtc actgatgtcg tgctcgccct cgacatccgg ctgcgagcgc
     8281 tggtcggcga tgccgtcggt cccgaaccgc tgccggtcga ggcgttgttg cagcgagtgt
     8341 ggccggtgct cgcatcgacg gctgccgacc cgctgacggc cgcgctcctc gagttgtccg
     8401 gcctcgccgc cgccggccac cagccctacg ccgaccttgc gccgggcctc atcgccggtt
     8461 gggtcgaccg gctcgtccga cagatcgtgc cctcggggcc gacgtcggcg cgcccggagg
     8521 catcggccgc tctggctcag ctctccgggc tcgtgaccct gcgccacctc agcggggccc
     8581 gcgccgccaa ctccgctgcc cgacacctcg gtgtgatcgc aactgcacca gtcgggcgca
     8641 cggggcggcg cgcgtgatca cccagcaccg acccgctccg gccaacgtcg tcatcctcgg
     8701 ttgcggtcgg agcggcacgt cgatcttcgg cgaactgttc gaggccattc cgggctacac
     8761 ct
//
LOCUS       DAOSOA010000115         8754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8000_length_8754_cov_6.109668, whole genome shotgun sequence.
ACCESSION   DAOSOA010000115 DAOSOA010000000
VERSION     DAOSOA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8000_length_8754_cov_6.109668"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PKY13_08735"
     CDS             complement(<1..632)
                     /locus_tag="PKY13_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80061.1"
                     /translation="MAARRFRYSPWDGTQRGFELDATDVMEQLTDDLLYHGDLNAALR
                     RMLQDGFNDRNGERIQGLREMLEKLRAERQERLDRFDLGGVFDDIAKELRDVVEQERA
                     ALDELAEEARTSGDERRRELTDQVVGERNMQLEMLPNDLAGQVKSLQNYDFTSSEARE
                     KFEELVDKLRQQLMQQAVDQMSGAMSDMSPEDMARMKDMLASLNEMLAQRA"
     gene            complement(642..2027)
                     /locus_tag="PKY13_08740"
     CDS             complement(642..2027)
                     /locus_tag="PKY13_08740"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOT80062.1"
                     /translation="MTEISADDFGDGFTWGVATAAYQIEGAWDADGKGPSVWDEFTHG
                     RGPLGLRRIKDGATGDVATDSYHRFEEDIALVAAMGFSAYRFSISWPRVMPDGTGAVN
                     AAGLDYYSRVVDACLAAGVEPWVTLYHWDLPAALERRGGWTNREIVGWFSDYVSVVVA
                     ALGDRVRHWMVFNEPLSFTMLGYLLGAHAPGRRGPAAFCAAAHHVNLATAEGARAARA
                     ASPRSDASIGTTQYLSPVLASGVGPLAGIAERSADALVNRLFLEPNLGLGYPWDDCGL
                     LRGIKRHHRPGDDELATIDLDFLGVQYYTRLRAPWLPIPGIWTIPHFGQDRGCELTSM
                     GWEVRPEGLGMVLDRVHAYGAYDRVVVTESGASFDEELVDGRVHDNRRIAYFQRHLAE
                     LCATIDRGVPVEGYFCWSLLDNFEWTFGYRPRFGLVHVDYATQKRVVKDSGRWFARLL
                     GGRAADAASQG"
     gene            complement(2037..3671)
                     /locus_tag="PKY13_08745"
     CDS             complement(2037..3671)
                     /locus_tag="PKY13_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80063.1"
                     /translation="MAGAGPVSPTRVFVLLKARLVVNGVRRMTRSTWARVGFVFSLLF
                     ALVAAGVGVGIALGLRVYWDADAQHRNLVLLSAGIVFGWWFAPVLGGGVDETIDPARL
                     SLLPLTRSQLRRGQIAAGFVGIAPVVMICWVIGLVVGSARTWASVPFVVVCGAVLLLA
                     ALVGSRALSTSLARLSRTRRGGDLAAVVAVLGGSAIFAGSQLLRFLTDEQLDAAARVL
                     RWSPPGMAGEAFELAGRGSFVAAGWRIAVLAAVVVLAGWWWSRQLDRLLVEPAQTGRS
                     ALRNDGEALAIFGGWRRRLPRTAAGAAAARELVYLTRSPGRRSALLGGTLLGLVYVVI
                     PVAWVGSPRTWLVLVAPVAMLLALQFASNQLGVDPAVFWIEVAAGPEPAARWVGRQLL
                     GCIAVLFPVLLAATLLAVWTGGWIEFGVVVITMVPATLAMVGVGSLLSPMLVTPVPDS
                     GNPFGGRQAMSGTGCSAVIVGLLYFVLVGVLIAPAEIALRWAWRSSSPLVAIAIGAGA
                     SAVNVGVWWWSTAAAVRQLPDRELEVLSLLDARLNT"
     gene            complement(3650..4474)
                     /locus_tag="PKY13_08750"
     CDS             complement(3650..4474)
                     /locus_tag="PKY13_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002547050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT80064.1"
                     /translation="MAEPIPSASVPSGSDEVGAAVESRSVVVRGLTKSFGDKVAVAGI
                     SLDVPRGSFYGLVGPNGAGKSTTIKMCTALLRPDAGDVWIDGQNVWADTHEAKRRLGV
                     LPDDLPLFERLTGDEFLTFLGMFRGLDPDVVASRTAELLDVLDLTEAARVLVADYSTG
                     MRKKISLAAAVLHGPRVLFLDEPFESVDPVSARTIQDLLRTFCSGGGTVVLSSHVMDT
                     VERLCDHVAIVSAGRIVVHGSVDDVRGGRRLEDVFIDAVGGVHLGAGSIGWLAQDR"
     gene            complement(4467..5867)
                     /locus_tag="PKY13_08755"
     CDS             complement(4467..5867)
                     /locus_tag="PKY13_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT80065.1"
                     /translation="MSRPGTLGELKSSGWESTSVKEEIRRNAIARIRAGEPLFPQVMG
                     YEDTVVPQLENALIAGHDIVFLGERGQAKTRMIRSLVELLDEWMPIIAGSEVNDDPLA
                     PISRYAIHLIAEHGDETRIDWVHRSDRYGEKLATPDTSIADLIGEVDPIKVAEGRYLS
                     DELTLHYGLVPRTNRGIFAINELPDLAERIQVGLLNVLEERDIQVRGYKIRLPLDVVL
                     VASANPEDYTNRGRMITPLKDRFGSQIRTHYPLEVATEVRIIEQEATPLVADGLTVDV
                     PDFMSEIIATFSHLARQSSHISQRSGVSVRLSVSNQESVVANAARRALRAGETHVVPR
                     ICDLEALPASTAGKVEIESLEEGRDGQIIDQLINGAVLSVFRERVAPEHLLNVVEAFD
                     GREPVEIGEDVPAAAYVQLCTELPALGRAASSLVAAETPEDIASAVELVLEGLHLSKR
                     LNKDAIGGRAQYRSRG"
     gene            5975..6751
                     /locus_tag="PKY13_08760"
     CDS             5975..6751
                     /locus_tag="PKY13_08760"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOT80066.1"
                     /translation="MRTSLRGRLVIASLTSLVTLTTTVGSAGAQADPSSTTTPETTTT
                     TTTTAPPAPPAPPAPVVLNYLPLSGATLAEGAKGSEIFLMQITLSARGFWLQDFPGNF
                     GASTRHALVAFQKYSGLPRTGKLDPLTRFVLGATLDRAQPKVPKAGRSIEVDIARQIL
                     TISDAGQVVWVFDVSTGKSSTPTPRGSYKLTRQINGQRVSNLGQLWRPKYFTGGYALH
                     GSPSVPNYPASHGCVRMTNQEINFLWDSNLAPIGTPVTLF"
     gene            6922..8571
                     /locus_tag="PKY13_08765"
     CDS             6922..8571
                     /locus_tag="PKY13_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOT80067.1"
                     /translation="MRVRRALVPVLGMLALVATACPEQPGGGGPTSTTTTSTTTTTLA
                     DNDGDGFNTGQDCNDNDPTINPGQAVDTVGDGVDSNCDGTDGIQTNTVFVATTGQDIS
                     TCGEALTPCATINQGLTRASSLGRTQVQVAGGTYAKFTVVAGFEIGGHYNSATWLKAG
                     GTTTFVTAAFDATVNGPVGILASGISTPTKVSDLTVSGVAAGPGQRSYGVAVLNSTSA
                     LTFDTVTVNGGTGGVGSAGANGTAGWSTAANAGSTGNNGFEPGGLGCNNTSAGAGGAG
                     GTGGSNGGTGGKGGVVDSPGCSNFLGVCSSGCDAQAGSTGATGGGPGGIGGAGGAAGT
                     SGFPGVCDLNGAPAFAGSAGAPGANGTAGSSGAGGTAGTAGGTGGLGSAGRGGGGGGG
                     GGANDCDQDDAGAGGGGGGAGGLRAATAGAGGQPGLAAISLYLSNSSPTLIGVQLNLG
                     TGGVGGTGGAGAAGQPGGGGGPGGSGYQVSGAGGPGGSGGTGGASGAGGGGGGGAAIG
                     LGTTGASSPVGTPTFSGGAGGAGGSGGNNGATGIVSNNATV"
BASE COUNT         1447 a         3185 c         2944 g         1178 t
ORIGIN      
        1 gcgcgctggg cgagcatctc gttgagcgag gccaacatgt ccttcatgcg ggccatgtcc
       61 tcgggggaca tgtcgctcat cgctcccgac atctggtcga ccgcctgctg catcaactgc
      121 tgacggagct tgtcgacgag ctcctcgaac ttctcccggg cctcggagct ggtgaagtcg
      181 tagttctgca acgacttcac ctgaccggcc agatcgttcg gcagcatctc cagctgcatg
      241 ttgcgctcac cgaccacctg atcggtgagc tcccgacgcc gttcgtcgcc cgacgtccgg
      301 gcctcctccg ccagttcgtc gagcgcggca cgctcctgct cgaccacgtc gcggagctcc
      361 ttggcgatgt cgtcgaacac cccgccgaga tcgaagcggt cgaggcgctc ctgccgttcg
      421 gcgcgcagct tctcgagcat ctcgcggaga ccctggatgc gttcgccgtt gcgatcgttg
      481 aagccgtcct gcagcatgcg ccgaagcgct gcgttgagat cgccgtggta gagcagatcg
      541 tcggtcaact gctccatgac gtcggtggcg tcgagctcga acccccgctg tgtgccatcc
      601 caaggtgaat agcggaaacg tcgagcagcc atgacgggac gctacccctg cgacgcagcg
      661 tctgcggcgc gaccgcccag caggcgggcg aaccaacggc ccgagtcctt cacgacccgc
      721 ttctgggtgg cgtagtcgac gtgcacgaga ccgaaccggg gccggtagcc gaacgtccac
      781 tcgaagttgt cgagcaggga ccagcagaaa tacccctcga cggggacacc gcgatcgatc
      841 gtcgcgcaca gttccgcgag gtgccgttgg aagtaggcga tgcgacggtt gtcgtgcacc
      901 cggccgtcga cgagctcctc gtcgaacgac gcacccgact cggtcacgac cacacggtcg
      961 tacgcgccgt aggcgtgcac ccgatccagc accatgccga gaccctcggg ccgcacctcc
     1021 caccccatcg acgtgagctc gcaccctcgg tcctgaccga agtgcgggat ggtccagatg
     1081 ccggggatcg gcagccacgg tgcccgcagc cgcgtgtagt actgcacgcc gaggaagtcg
     1141 aggtcgatcg tggcgagctc gtcgtcgccg ggacggtgat gccgtttgat cccgcggagc
     1201 aacccgcagt cgtcccacgg gtagccgagc ccgagattcg gttcgaggaa caggcggttg
     1261 accagtgcgt ccgccgaccg ttcggcgatg ccggcgagcg gaccgacccc actcgccaac
     1321 acgggcgaca ggtactgcgt cgtgccgatc gacgcgtcgc tcctcgggct cgcagctcgc
     1381 gccgcccggg cgccctcggc ggtcgcgaga ttgacgtggt gcgcggcggc acagaacgcg
     1441 gccgggcccc gtcgaccggg agcgtgtgcc ccgagcaggt agcccagcat cgtgaagctg
     1501 aggggttcgt tgaacaccat ccagtggcgc acccggtcgc cgagggctgc gaccaccacc
     1561 gagacgtagt cactgaacca gcccacgatc tcgcgattgg tccagccgcc ccggcgctcg
     1621 agcgcggccg gaaggtccca gtggtagagc gtgacccacg gttcgacccc cgcggcgagg
     1681 catgcgtcga cgacgcggga gtagtagtcg agcccggccg cgttgaccgc ccccgtgccg
     1741 tcgggcatca cccgcggcca cgagatcgag aagcgatagg cgctgaaccc catcgccgcg
     1801 accagggcga tgtcctcctc gaagcggtgg tacgaatcgg tggcgacgtc gcccgttgcg
     1861 ccgtccttga tccggcggag cccgagcggc ccccggccgt gggtgaactc gtcccacacc
     1921 gagggaccct tgccgtcggc gtcccaggcg ccttcgatct gatacgccgc cgtggcgacc
     1981 ccccaggtga aaccgtcgcc gaagtcgtcg gccgagatct ccgtcatcgc ccgaatctag
     2041 gtgttcaacc gagcgtccag gagcgagagc acctccagct cgcggtccgg cagctgacgc
     2101 accgccgccg cggtcgacca ccaccacacc ccgacgttga ccgcagacgc acccgcgccg
     2161 atggcgatcg ccacgagcgg agaactcgag cgccacgccc accgcagggc gatctcggcg
     2221 ggggcgatga gcaccccgac gagcacgaag tacagcaggc cgacgatcac ggccgaacac
     2281 cccgtgcccg acatggcctg ccgccccccg aacgggttcc cggaatcggg caccggcgtc
     2341 acgagcatcg gcgagagcag cgaccccacg ccgaccatcg cgagggtggc ggggaccatc
     2401 gtgatgacca ccaccccgaa ttcgatccac ccaccggtcc acaccgcgag gagggtggcg
     2461 gccaggagga cggggaacag cactgcgatg cagccgagga gctgacgccc gacccaccgg
     2521 gccgccggct cgggcccggc cgccacctcg atccagaaca cggccggatc gacgccgagc
     2581 tggttcgacg cgaactgcag cgcgagcagc atcgccacgg gagccaccaa caccaaccag
     2641 gtgcgggggc tcccgaccca cgcgaccggg atcaccacgt acacgagccc caacaacgtg
     2701 ccgccgagga gggctgaacg ccgtccgggc gagcgggtga gatacacgag ttctcgcgcc
     2761 gccgcggcgc cggccgcagt ccgcggcagc cgccgacgcc acccgccgaa gatcgccagc
     2821 gcctccccgt cgttccgcaa cgcgctgcga ccggtctgtg cgggttcgac gagcaggcga
     2881 tcgagctgac gagaccacca ccagcccgcg agcacgacga ccgcggcgag caccgcaatc
     2941 cgccacccgg cggcgacgaa actcccgcgg ccggccagtt cgaacgcctc accggccatc
     3001 cccggaggcg accaacgaag cacgcgcgcc gcggcgtcga gctgctcgtc ggtcaggaag
     3061 cggagcagct gggatccggc gaagatcgcc gacccaccga gtacggccac gaccgcggcg
     3121 agatcacccc cgcggcgcgt gcgcgacagg cgcgccaacg aggtggagag ggcacggctg
     3181 cccacgagcg ccgccaacag cagcaccgca ccgcagacca ccacgaacgg cacggacgcc
     3241 caggtccgcg ccgagcccac gacgagcccg atgacccagc agatcatcac caccggagcg
     3301 atcccgacga agccggcggc gatctgcccc cgccggagct gggaccgcgt caggggcaac
     3361 agcgacaggc gcgccgggtc gatcgtctcg tcgacaccgc cgccgagcac cggtgcgaac
     3421 caccagccga acacgatccc ggcggagagg agcaccagat tgcggtgctg ggcatcggca
     3481 tcccagtaca cccgcaggcc cagcgcgatg ccgacgccga cccccgctgc gaccagcgcg
     3541 aacaggaggg agaagacgaa gccgacccgc gcccacgtcg accgcgtcat cctccgcacg
     3601 ccgttgacga ccaaccgcgc cttcagcagc acgaacaccc gcgtgggact caccggtcct
     3661 gcgccagcca tccgatcgat ccggctccga ggtgcacccc tccgactgcg tcgatgaaga
     3721 cgtcctcgag tcggcgtcca cctcgaacgt cgtcgacgga cccgtgcacg acgatgcggc
     3781 cggcactcac gatggccacg tgatcgcaca ggcgttccac cgtgtccatg acgtggctcg
     3841 acagcaccac cgtgccgccg ccggagcaga aggtgcgcag caggtcctgg atcgtgcgcg
     3901 ccgacaccgg gtcgaccgac tcgaacggct cgtcgaggaa cagcacccgc gggccgtgca
     3961 acaccgcggc ggcgaggctg atcttcttgc gcatgcccgt cgagtagtcg gcgaccagca
     4021 cacgcgccgc ctcggtcaga tcgagcacgt cgagcaactc cgcggttcgc gacgccacga
     4081 cgtcggggtc gagaccccgg aacatgccga ggaacgtgag gaactcgtcg ccggtcagcc
     4141 gctcgaacaa cggcaggtcg tcggggagca cccccagccg ccgcttggcc tcgtgggtgt
     4201 cggcccacac gttctggccg tcgatccaga cgtccccggc gtcgggccgc aacaacgcgg
     4261 tgcacatctt gatcgtcgtc gacttgccgg cgccgttcgg tccgaccaac ccgtagaacg
     4321 atccgcgggg cacgtcgagg ctgatccccg cgacggcgac cttgtcgccg aacgacttgg
     4381 tgagaccgcg cacgaccacc gatcgggact cgaccgcggc accgacctcg tccgatcccg
     4441 acggcaccga cgccgacggg atcggttcag ccacgagacc ggtactgcgc ccgtcccccg
     4501 atggcgtcct tgttgagacg cttggagagg tggagccctt cgagcaccag ctcgaccgcg
     4561 ctggcgatgt cctcgggagt ctcggccgcc accagcgacg acgcggcccg accgagcgcc
     4621 ggcagctccg tgcagagctg cacgtacgcg gccgccggaa cgtcctcacc gatctcgacg
     4681 ggctcgcggc cgtcgaaggc ctcgacgacg ttgagcagat gctcgggcgc gacccgctcg
     4741 cggaacacgc tcaacaccgc accgttgatg agctggtcga tgatctgacc gtcacggccc
     4801 tcctcgagcg actcgatctc gacctttccg gcggtcgacg ccggcagcgc ctcgaggtcg
     4861 cagatgcggg gaacgacatg ggtctcgccg gcgcgcagtg ctcgccgcgc cgcgttggcc
     4921 acgacgctct cctggttgga caccgacaga cggaccgaca cacccgaccg ctggctgatg
     4981 tggctgctct gacgggcgag atggctgaac gtggcgatga tctccgacat gaagtcgggc
     5041 acgtcgacgg tgaggccgtc ggcgacgagc ggcgtcgcct cctgctcgat gatgcggacc
     5101 tcggtcgcga cctcgagcgg gtagtgcgtg cggatctggc tgccgaagcg gtccttcagc
     5161 ggggtgatca tgcgcccgcg gttggtgtaa tcctccgggt tcgccgaggc aacgagcacg
     5221 acgtcgagcg ggaggcggat cttgtagcca cgaacctgga tgtcgcgctc ctcgagcacg
     5281 ttgagcagac ccacctggat ccgctcggcg agatcgggga gctcgttgat ggcgaagatg
     5341 ccgcggttgg tgcgcggcac gagcccgtag tgcagcgtga gctcgtcgga gaggtaccga
     5401 ccctcggcga ccttgatggg gtcgacctca ccgatgaggt cggcgatcga cgtgtccggc
     5461 gtcgcgagct tctcgccgta acggtcgctg cgatgcaccc agtcgatccg ggtctcgtcg
     5521 ccgtgctctg cgatgaggtg gatcgcgtac cgcgagatcg gcgcgagcgg gtcgtcgttg
     5581 acctccgaac cggcgatgat gggcatccac tcgtcgagca gctcgacgag cgaacgaatc
     5641 atgcgggtct tggcctggcc ccgttcgccg aggaagacga tgtcgtggcc ggcgatgagc
     5701 gcgttctcga gctgggggac gacggtgtcc tcgtaaccca tcacctgggg gaacagcggc
     5761 tcgccggctc ggatccgagc gatcgcattg cgccggatct cctccttcac cgaggtggac
     5821 tcccaaccag acgatttcag ttcaccgagc gttccgggtc tactcatgtc ggcaacctac
     5881 cccatgggtt cgggccacgg tcgggcgccg aaccgaccgc tcccaggccc ttggacttac
     5941 cgctctgctc ccgcgagcgg taccctcgcc agccatgcgc acgtcactcc gcggccggtt
     6001 ggtcatcgcc tcgctcacct cgctcgtcac gttgacgacc actgtcgggt ctgcgggtgc
     6061 acaagccgat ccgtcctcga cgaccacacc ggagacgacg accaccacaa cgacgaccgc
     6121 tcccccggcc ccacccgctc cccccgcacc ggtggtgctc aactacctgc ccctctcggg
     6181 cgcgaccctc gccgaagggg cgaagggcag cgagatcttc ctcatgcaga tcacgctgtc
     6241 ggcccgcggt ttctggctgc aggacttccc cggcaacttc ggggcgtcga cgcggcacgc
     6301 gctggttgcg ttccagaagt actccggcct gccccgcacc gggaagctcg accccctcac
     6361 ccgcttcgtg ctcggcgcca ccctcgaccg cgcccagccg aaggtcccca aggccggccg
     6421 cagcatcgag gtcgacatcg ctcgtcagat cctcaccatc tccgacgccg gccaggtcgt
     6481 ctgggtgttc gacgtgtcga ccggcaagag ctcgacgccc accccgcgtg ggtcctacaa
     6541 gctcacccgt cagatcaacg ggcagcgggt ctccaacctg ggccagctgt ggcggcccaa
     6601 gtacttcacc ggcggctacg ccctgcacgg ctcgcccagc gtgccgaact atccggcgag
     6661 ccacggctgc gtgcggatga ccaaccagga gatcaacttc ctgtgggaca gcaacctggc
     6721 accgatcggc acgcccgtca cgctcttctg atcggccatc gcgtcggccc gacgggccag
     6781 ctgatcgagc ccccccccga acggggtgcc agatgacaga gtttgccgtc tcgtcacccg
     6841 agagacgttg acctttcgaa ctgttcaagt taggaaggaa ctctcgccgg ggccggcggg
     6901 aacgtccttg gagggatcag catgcgcgta cgtcgtgctc tcgtacctgt cctcggcatg
     6961 ctcgcgctcg tcgccaccgc atgccccgag caaccgggcg gaggcgggcc cacgtcaacg
     7021 acgaccacgt cgacgaccac gaccaccctc gccgacaacg acggcgatgg cttcaacacc
     7081 ggccaggact gcaacgacaa cgatccgacc atcaacccgg gccaggccgt cgacacggtg
     7141 ggcgacggag tcgactcgaa ctgcgacggc accgacggca tccagaccaa caccgtcttc
     7201 gtcgccacga ccggccagga catctcgacc tgcggcgagg cgctcacgcc ctgcgccacc
     7261 atcaaccagg gcctgacccg tgcgagctcg ctcggccgca cccaggtcca ggtggccggc
     7321 ggcacgtacg ccaagttcac cgtggtggcc ggcttcgaga tcggcggcca ttacaactcc
     7381 gcgacctggc tcaaggcggg cggcacgacg accttcgtga ccgccgcctt cgatgcgacc
     7441 gtgaacgggc ccgtcggcat cctcgcctcg gggatctcca caccgacgaa ggtctccgac
     7501 ctcaccgtca gtggcgtcgc ggccgggccg ggccagcgct cctacggcgt cgccgtgctc
     7561 aactcgacct cggcgctcac gttcgacacc gtcaccgtga acggcggcac cggcggcgtg
     7621 ggctctgccg gggccaacgg aaccgccggg tggagcacgg cggccaacgc cggcagcacc
     7681 ggcaacaacg gcttcgaacc gggcggcctc ggatgcaaca acacgagtgc cggcgccggt
     7741 ggcgccggcg ggaccggtgg cagcaacggt gggaccggcg gcaagggcgg cgttgtcgac
     7801 tcgcccgggt gcagcaactt cctcggtgtc tgcagcagcg gctgtgatgc gcaggccggg
     7861 tcgaccggcg cgacgggcgg tggccccggt ggcatcggcg gcgccggcgg cgctgccggg
     7921 acgagcgggt tccccggtgt gtgcgacctg aatggtgctc cggcattcgc cggcagcgcg
     7981 ggtgcgcccg gggccaacgg caccgcgggt tcgtcggggg ccggaggcac tgcgggtact
     8041 gccggcggca ccggcgggct cggctccgcc ggccgaggtg gcggcggcgg cgggggcggc
     8101 ggcgccaacg actgtgacca ggacgatgcc ggggccggcg gtggcggcgg cggtgccggc
     8161 ggccttcgcg ctgcgacggc gggtgcgggc ggccaacccg gcctcgccgc catctcgctc
     8221 tacctcagca actcgagccc gacgctgatc ggtgtgcagc tcaacctcgg caccggcggc
     8281 gtcggcggca ccggcggcgc cggcgcggcc ggtcagcccg gtggcggcgg cgggcccggc
     8341 ggatcggggt accaggtgag cggtgccggc ggtcccggag ggtcgggcgg gaccggtgga
     8401 gcatccggtg ccggcggcgg aggaggcggc ggcgccgcga tcgggctcgg cacgaccggt
     8461 gcgagcagcc ccgtgggcac ccccacgttc agcggtggcg ccggcggggc cggtggctcg
     8521 ggtggcaaca acggcgccac gggcatcgtg tccaacaacg cgacggtctg acgcccaacg
     8581 acgactacga cgcgtcgaca acatcgagaa cgaaccggag ccgccctgga gggcggctcc
     8641 ggtcgcgtcc gggcactgct gcccacaacc ccgggggcaa aggttcccga tccattgctg
     8701 gccctacgcg atgaagctgg gatgacggtg ttggtcgggg tccaccggtg atgg
//
LOCUS       DAOSOA010000116         8704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8063_length_8704_cov_5.813273, whole genome shotgun sequence.
ACCESSION   DAOSOA010000116 DAOSOA010000000
VERSION     DAOSOA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8063_length_8704_cov_5.813273"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="PKY13_08770"
     CDS             <1..1672
                     /locus_tag="PKY13_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HOT80068.1"
                     /translation="TAMVDEAMLTLPERIERAAALGGTVAFVDGDDHELLTWAELHED
                     ARSVAAGLQSLGVGEGDHVALMGPTTRTLVTTIQAVWLAGGVLVMLPLPMRMGSIDQF
                     IEQTRDRIRFAKASTVVLDPQLAEFIEHQPGDPTFLTYADLLPEATGQTAEDWKRPTP
                     DPDALAVLQFTSGSTSEPKGVMLAHRQLCANIDGCYEAAQMSLDDTVVSWLPLYHDMG
                     LIGLLTIPMTTGAGLVQGAPQDFLSKPARWMRWISDLDGTITAGPNFSFALATRSLNR
                     SEGLDLSRLRICLNGAEPIDADGFRTFLAAGARFGMNPGAAFPAFGMAEVCIAGSFPV
                     PGTGFETDVVDGAALEHEHVARPVEPGTEGARELAKLGFPIPGLELRIVDPSTGAARG
                     DREVGELQIRGTSLTLGYYERPEATDELFTDGWLHSGDLAYMVDGQVVVCGRIKDVII
                     IGGRNIYPQDIEKVVGGIDDVRTGNVIAFGEEGAHAKQHIVVVAETRSDDPAALAKVI
                     SRKVTEDIGVPPRDVVLVPPGTIPKTSSGKLQRSACRASYQAGEYQTV"
     gene            complement(1743..2765)
                     /locus_tag="PKY13_08775"
     CDS             complement(1743..2765)
                     /locus_tag="PKY13_08775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT80069.1"
                     /translation="MKVDGGLGPDLSHDLGTFAAQAAEQEAAGYNGIWTAETNHDPFF
                     PLLLAAGTTSDVDLGTGIAVAFSRSPMTLAATANDLQAFTGGRFILGLGSQIKPHIEK
                     RFSMPWSHPAARMREFILAMRAIWACWNDGTKLDFQGDFYTHTLMTPFFDPGPNPHGA
                     PRVFLAGVGEKMTEVAGEVCDGFICHAFTTERYLREVTIPALERGLDASGRTLADIEI
                     SGPAFVVTGSDEESFEKSKAGTRQQIAFYGSTPAYRGVLDLHGWGDLQPELNAMSKQG
                     KWKEMGELIDDDMLDTFAVVAEPSGIAPAFTARFGDVVDRLSFYAPYKNDPDTWLPVI
                     EALQKA"
     gene            2935..5010
                     /locus_tag="PKY13_08780"
     CDS             2935..5010
                     /locus_tag="PKY13_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT80070.1"
                     /translation="MFDVRSVVRAVAPAAAIIVLQLVLWPVSAGTFVSGLILGGLGAL
                     IALGMALIYRANNVVSFAQADLGSLPATAAIVVMEVWGFTYWMSLVGGLLAAALVGAI
                     VELAFIRRFFSAPRLIVTVATIGVGQFLFFGSAMFPQIWHLTPTIRTFDPPFTFRLTI
                     GAVLFDANDLMAAIVAPIILIGLTAFLRTTDTGVAIRAAASAPDRASMLGIPVRRLQT
                     QVWVIAAVMAFLGVFLQAGVTGLPPGGLFTWSILLRALAALVMGNMTNLGAIALSSIA
                     LGILQNAMERANDGALVGPVLLAVILVALLARRRGTSRLAAHEQGSWALAGEFHAIPA
                     HLAKLPQVRAFRAAVGITIAAVVLVAPEVLGTNGLIKAGAMLVFATIGLSMVVLSGWA
                     GQVSVGQMAFVGLGGAVAAWAIVERGYDPVLAMGMAGLAGAALAIVIGIPALRLRGLN
                     LAVVTLGLAVAASNALFSNELWRWIPTGVFDRPKLLGRFDVDSPGRLYYLSLVVLVIS
                     VVAVRGLRRSRFGRVLVAQRDNEVAVSSFGANVVTAKLASFAVSGFIAAVAGAVFVLH
                     QAAFRDENYDVGFGISVFVAAVIGGLGSPLGGILGAVYLRGAQWLLPGNWQILASSVG
                     VLLVLLMIPDGLAGLWYRTRDQLIRAMAGAAADSQPDPDDAIASSANGSAPPASGADV
                     EPELAEVTE"
     gene            5007..8066
                     /locus_tag="PKY13_08785"
     CDS             5007..8066
                     /locus_tag="PKY13_08785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT80071.1"
                     /translation="MSARLDSLKPYVEGMFSPRRWWATIRHPVDALRSVAGDGPVFAL
                     VVLFGLNAVDELDRTGFGILIPTIRDHFGMSDTGILSLVALTALGALLLQLPIAWWAD
                     RGSRVRLAMFGGIAWGVFSFMTGMSATVWMLVLVRSGAGIGRAVVDPTHSSLLSDYYP
                     VERRPAVFSFHRSANAVGQFVGPLAAGALSNAFGWQVPFFVFAVPTVVFVILAMRLKE
                     PIRGAQERKAMGASQDAIDTEEPSASFGEAWRMVWKIESLRRIWYAIPFLAVSIIGFV
                     SLAGLLYDRVYQYDDLQRGILASLVEPFQLVGLAIGGRVGVRLLMKDPALVFRFLRYV
                     AFACGAWSALFAWSPNIVVTVIANIGLTATLAILLPGILASLSMAIPARARAVGFSVA
                     SYWAIPGLIFVPAIGWVSDNWGIRWGMLLMTPLMVAGGVMISTVASSIKRDIDDVWTA
                     SSTRSQALFQRRKGLSKLLLVKDLNVSYGSVQVLFDVSVEIGEGEVVALLGTNGAGKS
                     TLLKAISGIAEADFGAVIFDGRDITHAPPHEIAVLGVGQMPGGHGVFPTLTVGENLRA
                     AGWQSRRAKAQYAVRVEQALDAFPILRTRHEEPAANLSGGQQQMLALAMNLVNAPRLL
                     MIDELSLGLAPVVVERLAEMVREVAAAGTTVILVEQSVNVALTLADTAYFMEKGEIRF
                     SGPTAELLERPDLLRSVFLGKAVEAAARPTAAVVADGTDTADADNGNELEAGVALATH
                     DLTVTFGGIRAVDGVDIEVGRREIVGLIGPNGAGKTTLFDLICGFTAMDRGRVVLDGR
                     DVAGLPPHRIARLGLGRSFQDAALFSSLTVEETIAVALDRWVAVKDPISAMLRLPGSV
                     ESEDHVLRRVDELIELFDLGRFRERFIGELSTGSRRIVDLACVVAHSPSVLLLDEPSS
                     GIAQRETEALGPLILRLRDELGCAIVVIEHDMPLLASICDRLVALESGALVAAGPVAD
                     VLADERVVASYLGTTPEVIARSGSLTDRPPSSNGSANGSSPTGGTHE"
     gene            8059..>8704
                     /locus_tag="PKY13_08790"
     CDS             8059..>8704
                     /locus_tag="PKY13_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80072.1"
                     /translation="MSDTNGSGLRPSERPPLSPAKKYGPLAIVAAVVIAVVAVIATSS
                     GDDETADRTPVTVDQGAKTLADVDNLPDGVETFARAEKLGTAGSIDWGERCDTSVGKV
                     KMPMWPQPDCFKPFTGDNGGATATGVTADTIKVVVWLAQPEDPVLKFIYAQIGNNDTP
                     DQTFETFEGFAEIFNTYYETYGRKVELIRYNATGPINDSTANRRRRDHRPRHQAV"
BASE COUNT         1229 a         3065 c         2932 g         1478 t
ORIGIN      
        1 gacggcaatg gttgacgagg caatgctgac acttcccgag cggatcgaac gggccgcagc
       61 gctcggcggg acggtggcgt tcgtcgacgg ggacgatcac gaactcctga cctgggccga
      121 actgcacgag gacgcccgct ccgtcgctgc cggcctccag tcgctgggcg tcggcgaggg
      181 cgaccacgtc gcgctcatgg ggccgaccac ccgcaccctc gtcacgacca tccaagcggt
      241 ctggctcgcg ggcggggtgc tcgtgatgtt gccgctcccg atgcgaatgg gctcgatcga
      301 ccagttcatc gaacagaccc gtgaccgcat ccggtttgcg aaggcgtcca cggtcgtgct
      361 cgatccgcag cttgccgagt tcatcgaaca ccagccgggc gacccgacgt tcctcaccta
      421 cgccgacctc cttcccgagg ccacgggcca gaccgccgag gactggaagc gcccgacgcc
      481 ggatcccgac gccctcgccg tcctccagtt cacgagcggg tccacctccg agcccaaggg
      541 cgtgatgctc gcccatcgtc agctctgcgc caacatcgac ggctgctacg aggcggcgca
      601 gatgtcgctc gacgacaccg tcgtgtcgtg gctgccgctc taccacgaca tggggctgat
      661 cgggctcctg accatcccga tgacgaccgg cgccggtctg gtgcagggcg ctccacagga
      721 cttcctctcc aagccggcgc ggtggatgcg gtggatctcc gacctcgacg gcaccatcac
      781 cgcggggccg aacttcagct tcgcgctcgc aacccgctcg ttgaaccggt ccgagggcct
      841 cgacctgtcc cgcctgcgca tctgcctcaa cggcgccgag ccgatcgacg ccgacgggtt
      901 ccgcaccttc ctcgctgccg gcgcccgctt cggtatgaac cccggcgcgg cgttcccggc
      961 gttcggcatg gccgaggtgt gcatcgccgg gtcgttcccc gttcccggaa ccggtttcga
     1021 gaccgacgtc gtcgacggtg ctgcgctcga acacgaacat gtcgcccggc cggtcgaacc
     1081 gggcaccgag ggcgcccggg agctggccaa gctcggcttt ccgatccctg gcctcgagct
     1141 ccgcatcgtc gatccgtcga ccggcgcggc gcggggcgac cgcgaggtcg gcgagctaca
     1201 gatccgcggc acatccctca cgctcgggta ctacgagcgg ccggaggcga ccgacgagct
     1261 gttcaccgac gggtggctcc acagcggtga cctcgcctac atggtcgacg gccaggtcgt
     1321 ggtgtgcgga cgcatcaagg acgtcatcat catcggcggc cgcaacatct acccccagga
     1381 catcgagaag gtcgtcggtg gcatcgacga cgtccgcacc ggcaacgtca tcgcgttcgg
     1441 cgaggaaggc gcgcacgcca aacagcacat cgtggtcgtc gccgagaccc gctccgacga
     1501 ccctgccgcg ctggcgaagg tcatctctcg caaggtcacc gaggacatcg gtgtgccgcc
     1561 acgcgacgtt gtgctcgtcc cgccgggcac catcccgaag acctcctcgg gcaagctcca
     1621 acggtccgcc tgccgcgcct cctaccaggc cggcgagtac cagaccgtct gacgggtttt
     1681 ccgggtagtt gtcctcacct gctgaggaca actccccgcg aaactcgagc gaaggcctct
     1741 cgctacgcct tctggagcgc ctcgatgacc gggagccagg tgtcggggtc gttcttgtag
     1801 ggggcgtaga agctgaggcg gtcgacgaca tcgccgaaac gggcggtgaa cgccggtgcg
     1861 atgcccgagg gctcggcgac gacggcgaac gtgtccaaca tgtcgtcgtc gatcagttcg
     1921 cccatctcct tccacttgcc ctgcttcgac atggcgttga gctcgggctg caggtcgccc
     1981 catccgtgga ggtcgagcac gccgcggtac gcgggcgtcg agccgtagaa cgcgatctgc
     2041 tggcgggtgc ctgccttcga cttctcgaac gactcctcgt cggacccggt gacgacgaag
     2101 gccgggccgg agatctcgat gtcggcgagc gtgcggcccg acgcatcgag gccgcgttcc
     2161 agcgcgggaa tcgtgacttc gcgcaggtag cgctcggtcg tgaaggcgtg gcagatgaag
     2221 ccgtcgcaca cctcgccggc gacctcggtc atcttctcgc cgacaccggc gaggaagacc
     2281 cgcggcgcac cgtgaggatt ggggccggga tcgaagaacg gcgtcatgag ggtgtgggtg
     2341 tagaagtcgc cctggaagtc gagcttggtg ccgtcgttcc agcacgccca gatggcacgc
     2401 atcgccaaga tgaactcgcg catgcgggct gcggggtgcg accacggcat cgagaagcgc
     2461 ttctcgatgt gtggcttgat ctggctcccg agccccagga tgaagcggcc gccggtgaac
     2521 gcctgcaggt cgttggccgt cgcggcgagc gtcatcgggc tgcgggagaa cgccaccgcg
     2581 atcccggtgc cgaggtcgac gtcggacgtg gtgccggcgg ccaacaggag cgggaagaac
     2641 ggatcgtggt tcgtctccgc cgtccagatg ccgttgtaac cggcagcttc ctgctcggcc
     2701 gcctgcgcgg cgaacgttcc gagatcgtgt gagaggtcgg gcccgagccc gccgtcaacc
     2761 ttcatcgcat ctgccccctg gtgatgtccg gcgctcacac tacgtcgtgc gcgaacagcc
     2821 ggcatgaggt gtcacccgtc ggtatacact tcgccctcgt tacggagaga cagtttcagg
     2881 gacagaccgt cgccgacttc ggggggagca gcgtgatcgg ggtgcgttcg accgatgttt
     2941 gacgtccgtt ccgtggttcg agcggtcgcg ccggcggcgg cgatcatcgt cctccaactc
     3001 gtgctctggc cggtgtcggc gggcacgttc gtgtcggggc tcatcctcgg cgggctcggc
     3061 gccctcatcg cgttgggcat ggccctgatc taccgggcga acaacgtcgt gagcttcgcc
     3121 caggcggacc tcggctccct gccggcgacc gccgccatcg tcgtgatgga ggtctgggga
     3181 ttcacctact ggatgtcgtt ggtcggtggc ctgctggcgg cggccctcgt cggagcgatc
     3241 gtcgagctcg cgttcatccg tcggttcttc agcgccccac gcctgatcgt cacggtcgcg
     3301 accatcggcg tcgggcagtt cctgttcttc gggtcggcga tgttcccgca gatctggcac
     3361 ctcaccccga ccatccggac gttcgacccg ccgttcacgt tccgtctgac gatcggcgcc
     3421 gtcctcttcg acgccaacga cctgatggcg gcgatcgtcg caccgatcat cctcatcggc
     3481 ctcaccgcct tcctccggac gaccgacacc ggcgtcgcca tcagggccgc cgcgtcagcg
     3541 cccgaccgcg catcgatgct cggcatcccg gtgcggcgtc tccagaccca ggtgtgggtc
     3601 atcgctgcgg tgatggcgtt cctgggcgtg ttcctccagg cgggggtcac cggcctgccg
     3661 ccgggcggcc tgttcacgtg gtcgatcctc cttcgggcgc tcgccgcgct cgtcatgggc
     3721 aacatgacga atctgggtgc gatcgcactg tcgtcgatcg ccctcggcat cctgcagaac
     3781 gccatggaac gggcgaacga cggtgcgctc gtgggccccg tactgctcgc cgtgatcctc
     3841 gtcgcgctgc tggcccgtcg acggggcacc agccgactcg ccgcccacga gcagggatcg
     3901 tgggcgctgg ccggcgagtt ccacgccatt cccgcgcacc tcgcgaaact gccgcaggtc
     3961 cgggcgttcc gcgctgccgt cggcatcacg atcgcggcgg tcgtgctggt cgcccccgag
     4021 gtgctcggca ccaacgggct catcaaagcg ggagcgatgc tcgtgttcgc cacgatcggc
     4081 ctgtcgatgg tggtgctgtc tggctgggcc gggcaggtgt cggtgggcca gatggcgttc
     4141 gtcggcctcg gcggcgccgt cgcagcgtgg gcgatcgtgg agcggggcta cgacccggta
     4201 ttggcgatgg gcatggcggg gctcgccggc gcggcgctcg ccatcgtgat cggcatcccc
     4261 gcgttgcgcc ttcgtggcct gaacctcgcc gtcgtcacac tcggtctcgc ggtggcagcg
     4321 tcgaacgcgc tgttctccaa cgagctgtgg cggtggattc ccaccggggt gttcgaccgg
     4381 ccgaaactgc tcggccggtt cgacgtcgac tcgcccggtc gcctctacta cctgtccctc
     4441 gtggtcctcg tgatctcggt ggtggcggtg cgtggcctcc ggcgcagccg gttcggtcgc
     4501 gtgctcgtcg cccagcgcga caacgaggtc gccgtcagct cgttcggggc gaacgtcgtg
     4561 acggccaagc tggcgtcgtt cgcggtctcg gggttcatcg ccgcggtcgc cggcgccgtg
     4621 ttcgtgctcc accaggccgc gttccgagac gagaactacg acgtcggatt cggcatcagc
     4681 gtgttcgtcg ccgcggtgat cggcggactg ggcagcccgc tcggcgggat cctgggcgcg
     4741 gtctacctcc ggggagccca gtggctcctg ccgggcaact ggcagatcct cgcctcatcg
     4801 gtgggggtgc tcctcgtgct gttgatgatc cccgacgggc tggcaggcct ctggtaccgc
     4861 acccgtgacc agctgatccg ggccatggcc ggtgccgctg ccgacagcca gcccgaccca
     4921 gacgacgcga tcgcttcgtc cgccaacggc agcgcgcctc cggcgagcgg agccgacgtc
     4981 gagcccgaac tcgccgaggt gaccgaatga gcgcacgcct cgactcgctc aagccgtacg
     5041 tcgagggcat gttcagcccc cgccggtggt gggcgaccat ccgccacccg gtcgacgcgc
     5101 tgcgatccgt cgccggcgac ggcccggtgt tcgcgctcgt tgtcctgttc gggctgaacg
     5161 cggtggacga gctcgaccgc accgggttcg gcatcctcat cccgacgatc cgcgaccact
     5221 tcggcatgag cgacaccggg atcctgtcgc tcgtcgcgct caccgcgctc ggcgcgctgc
     5281 tgctccagct cccgatcgcc tggtgggccg accgcggctc ccgggtccgg ctcgccatgt
     5341 tcggtggcat cgcctggggt gtgttctcgt tcatgaccgg gatgtccgcc acggtgtgga
     5401 tgctcgtgct ggtgcgctcc ggagccggca tcggtcgggc cgtggtcgac ccgacccaca
     5461 gttcgctcct gtcggactac taccccgtcg agcgccggcc cgccgtgttc tcgttccacc
     5521 ggtcggccaa cgccgtcggc cagttcgtcg gaccactggc cgccggcgcc ctctcgaacg
     5581 cgttcggatg gcaggtgccg ttcttcgtct tcgccgtccc caccgtggtc ttcgtgatcc
     5641 tcgcgatgag gctgaaggag ccgattcgtg gcgcccagga gcgcaaggcg atgggggcgt
     5701 cgcaggacgc catcgacacc gaggagccgt cggcttcgtt cggcgaggcg tggcggatgg
     5761 tgtggaagat cgagtcgctg cgacgcatct ggtacgccat cccgttcctc gcggtctcga
     5821 tcatcggatt cgtctcgctc gccgggctgc tctacgaccg ggtgtaccag tacgacgatc
     5881 tgcagcgcgg catcctggcg tcgctcgtcg aaccgttcca gcttgtcggg ctcgccatcg
     5941 gcgggcgtgt cggtgtgcgc ctgttgatga aggacccggc gctggtcttc cgattcctcc
     6001 ggtacgtggc gttcgcgtgc ggtgcgtggt cggccctgtt cgcctggtca cccaacatcg
     6061 tggtgacggt catcgccaac atcggcctca cggccaccct cgccatcctg ctgcccggca
     6121 tcctggcgtc gctgtcgatg gcgatcccgg cgcgggcccg ggccgtcggc ttctcggtcg
     6181 cctcctactg ggcgattccc gggctcatct tcgtgccggc catcgggtgg gtgtcggaca
     6241 actggggcat ccgctggggc atgttgctga tgacaccgtt gatggtggcc ggcggcgtga
     6301 tgatctccac cgtcgcgtcg tcgatcaagc gcgacatcga cgacgtgtgg acggcatcgt
     6361 ccacccgcag ccaggccctc ttccagcgac gcaagggcct gtcgaagctg ctcctcgtca
     6421 aggacctcaa cgtctcgtac ggcagcgtgc aggtgctgtt cgacgtgagc gtcgagatcg
     6481 gtgagggcga ggtcgttgcg ctgctcggca ccaacggcgc cggcaagtcc accttgctca
     6541 aggccatctc gggcatcgcc gaagccgact tcggcgccgt gatcttcgac gggcgggaca
     6601 tcacccacgc cccgccgcac gagatcgcgg tgctcggggt ggggcagatg ccgggcggtc
     6661 acggtgtgtt cccgacgttg accgtcggcg agaacctcag ggccgccggg tggcagtcga
     6721 ggcgggcgaa ggcgcagtac gccgtccggg tcgagcaggc gctcgacgcg ttcccgatac
     6781 tccgcacccg ccacgaggaa cccgctgcca acctgtcggg cggtcagcag cagatgctgg
     6841 cgttggcgat gaacctcgtc aacgcgccgc ggctcctgat gatcgacgag ctctcgttgg
     6901 ggctggcgcc ggtcgtcgtc gaacgcctcg ccgagatggt gcgagaggtc gctgccgccg
     6961 gtacgacggt gatcctcgtc gagcagtcgg tcaacgtggc gctcacgctc gccgacacgg
     7021 cgtacttcat ggagaagggt gagatccggt tctccggccc gaccgccgaa ctcctcgaac
     7081 ggcccgatct cctgcgctcg gtgttcctcg gcaaggccgt cgaggcggcg gcccggccga
     7141 ccgcagcggt cgttgccgac gggaccgaca cggccgacgc ggacaacggc aacgagctcg
     7201 aggcaggggt cgccctcgcc acccacgacc tcaccgtcac gttcgggggc atccgagccg
     7261 tcgacggcgt cgacatcgag gtgggccgcc gagagatcgt cggcctgatc ggccccaacg
     7321 gggcaggcaa gacgaccctg ttcgacctca tctgcgggtt caccgccatg gaccgcggtc
     7381 gcgtcgtgct cgacggtcgc gacgtcgccg ggctcccgcc ccaccgcatc gcccggctcg
     7441 ggctcggccg ctcgttccag gacgccgcgt tgttctcgtc gctcaccgtc gaggagacga
     7501 tcgccgtggc gctcgaccgc tgggtcgccg tgaaggaccc gatcagcgcg atgctgcgcc
     7561 tgcccggttc ggtcgagtcc gaggaccacg tcctgcgtcg cgtcgacgaa ctgatcgaac
     7621 tgttcgacct cggccgattc cgggagcggt tcatcggcga gctgtcgacc ggctcgcggc
     7681 gcatcgtcga cctcgcctgc gtcgtcgccc actcgccgtc ggtgctcctg ttggacgagc
     7741 cgtcgtccgg catcgcccaa cgcgaaaccg aagcgctcgg gcccctcatc ttgcgccttc
     7801 gcgacgagct cgggtgcgcc atcgtcgtca tcgaacacga catgccgttg ctcgcgtcga
     7861 tctgtgaccg cctcgtcgcg ctggagtcgg gcgcgctggt cgcagccggc cccgttgccg
     7921 acgtgctcgc cgacgagcgc gtggtcgcct cgtacctcgg cacgaccccc gaggtgatcg
     7981 cccgttccgg gtcgctcacc gaccgcccac catcgtccaa cggatcggcg aacggcagct
     8041 cgccaacagg aggaacacat gagtgacacc aacgggtccg gcctccgacc gagcgagcgc
     8101 ccgccgctga gtcctgcgaa gaagtacggc ccgttggcca tcgtggcggc cgtcgtgatc
     8161 gcggtcgtcg ccgtcatcgc cacgtcgtcg ggcgacgacg agacggcgga tcgcacgccg
     8221 gtcaccgtcg accagggcgc caagacgctc gcggacgtcg acaacctgcc cgacggggtc
     8281 gagacgttcg cccgcgccga gaagctgggc acggccggct cgatcgactg gggcgagcgg
     8341 tgcgacacgt cggtcggcaa ggtgaagatg ccgatgtggc cccagccgga ctgcttcaag
     8401 ccgttcaccg gtgacaacgg cggagcgacc gccactggcg tcaccgccga cacgatcaag
     8461 gtcgtcgtgt ggctcgccca acccgaggac ccggtcctca agttcatcta cgcccagatc
     8521 ggcaacaacg acacgccgga ccagaccttc gagaccttcg aggggttcgc cgagatcttc
     8581 aacacctact acgagaccta cggccgcaag gtcgagctca tccgctacaa cgccaccggg
     8641 cccatcaacg actcgaccgc caaccgccga cgccgagacc atcgcccgcg acatcaagcc
     8701 gttc
//
LOCUS       DAOSOA010000117         8519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8249_length_8519_cov_8.870392, whole genome shotgun sequence.
ACCESSION   DAOSOA010000117 DAOSOA010000000
VERSION     DAOSOA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8249_length_8519_cov_8.870392"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKY13_08795"
     CDS             <1..431
                     /locus_tag="PKY13_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80073.1"
                     /translation="YRFACDQFTSRGYRNTLDGWQTNMRAIALGMESLRRLDRYGIAA
                     GGEQYVGFEALPAGRAMPAGGDTMTVQAAAEILAAEAIDPAVSAVPIFTAAELLSQPV
                     ARVSAYRMAVRRLHPDQGGEPAAFVRLNTARDVLGEYVHS"
     gene            531..1379
                     /locus_tag="PKY13_08800"
     CDS             531..1379
                     /locus_tag="PKY13_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80074.1"
                     /translation="MKSIRFPKFMIAAAVIVGAASITTAAPASASTVTGSDVTVSRTV
                     KADQSAPCVDPLFSLSNYVNSTADAFTLTVNVNAPLCSPLEAKAVIYAMPGDGVAWPQ
                     ELVKTVPFTLSEAGTVTVTFSKGCGATQFDVITGHAPQTINTGFDHGPLLFPAQLQTA
                     QQFWGARCVDETTTTSTVPATTSTVPTSSTTTAATSTSTTAAEAKTTTTAASADVNIS
                     ATSTVAEPTTTLAIAVAGQSVDNPVPATSLAFTGSATNIIAALAIGLVLIGISALVIR
                     ERRNSK"
     gene            1480..2133
                     /locus_tag="PKY13_08805"
     CDS             1480..2133
                     /locus_tag="PKY13_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80075.1"
                     /translation="MSSPARHPKGTPSSLGGQFRSTDHHESDVALADAGTDERSTRER
                     AEAALSWFAMGQDTPPTVELIDDYVAVRLDECTISLTIGMDDGGVTVTHLDGEEFGLA
                     EYLEPFDHWTADERDGVGLAVQSCMYDLASWANAGAADAVASLNASLGRTDPEVLEKY
                     ALLVDEWDYSHTRLAELADGDSTGVWRDELEQSTERGFDLLRRIAAATRAAHDGRVC"
     gene            2167..2973
                     /locus_tag="PKY13_08810"
     CDS             2167..2973
                     /locus_tag="PKY13_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOT80076.1"
                     /translation="MKCMNAPVDLTAYDLILINTSGGKDSQAMLSYVHELATEVGIAD
                     RLVAVHADLGRVEWPGTAELAETQAAAYGIRFVKVSRPQGDLLTHIEQRGMFPSSTTR
                     YCTSDHKRGQIRKVMTALADEHRAAGGGRIRILNCIGLRREESPARARRVEFVPEGPA
                     SNGRRRVDEWSPIIDWSTADVWDTIRSTGLPHHPAYDAGMGRLSCTFCVLASRSALVR
                     AAQLAPELAAEYAAVEARIGHRFNHKLSMAEIIAAAAVSETPVEVSAWVA"
     gene            3087..4157
                     /locus_tag="PKY13_08815"
     CDS             3087..4157
                     /locus_tag="PKY13_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80077.1"
                     /translation="MSQRAGFDDGDRHGWNAVFCDRLERMMHGRAAADLAAAPGALVT
                     LPMFLWVPGVAAGRKVNATGPTGGGLRLPVRMTWSCGHAVIVGRDAPVTVRWKAAGLG
                     ISASGTTSITAGAGAPAKRRTTVGPAVVCDDLGAPADIIGVWRTKTANGQFLGALVSD
                     GHAASWEARMSIEGYVDRACRLAADFIAREIAEFRGAPTATSVVSDITLENIVTRMLV
                     GTSSDGATGERTGSVTNLLAKSLEPATFWRVDPQRFVRVWLQRDAESEVRRAIGDPWI
                     APKVRKVATATAAASSRRKYPFRPKTQLKKTKELYNKKYPADRLGDRRLETALTPASD
                     PNVDAVDLEQFAEETAENRLAV"
     gene            4220..4564
                     /locus_tag="PKY13_08820"
     CDS             4220..4564
                     /locus_tag="PKY13_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80078.1"
                     /translation="MRAEWSVGVAEGLLTSVDVIEHACTVEGRPLRRMKLRQLLLSQP
                     HWGQARTGEAMTRFARFAGLAPSADLDELTVGWLIDSRAGGRRISAWLDAVEVRRDGP
                     PWPGFPYLRKPA"
     gene            4567..5412
                     /locus_tag="PKY13_08825"
     CDS             4567..5412
                     /locus_tag="PKY13_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80079.1"
                     /translation="MDTDASHAALTIIHQTTDPGRRRRLVEDFHRERIDFYTAVASKG
                     ARRYGAPRSQIPDLAQEVGMLAAKMVESWDPSLMKFEAMLHVVASRHIRELVNSPAWV
                     GVSGASLLNRRRGHLIATQRRLAREHGVEPTDEQVVEAWNREAKTRFGDPGRAGMIAS
                     LSDFTGVSVMPREPQGDSDSTAWASPGSPDMLADTSVLSAEESPEFVAAVIEAARSVS
                     DELGTVAEAWLGSWGPGETRRCAEIREVAAALGQKYEWVRRRVNQLKVLAVEVAVTDF
                     GITSP"
     gene            complement(5399..6016)
                     /locus_tag="PKY13_08830"
     CDS             complement(5399..6016)
                     /locus_tag="PKY13_08830"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80080.1"
                     /translation="MQHNTLAARRGRDLAVLDGLDIGDEKRRLLDEFTDRWPWETVDR
                     TYAVRRLVSLTPARYRDDIRQVAAIAAWRAVESWDPEQRSLVGWCHFKGHYAALDALR
                     ALYRDEPPAEPGAEQVATDGDPEGAVEGYSADEILDQLRSIPGLVTERHLRVLQLITA
                     DKSWSQKAIADHYGVSESAISQTLRDLRNRLTPDVVRRLPALMER"
     gene            complement(6150..6575)
                     /locus_tag="PKY13_08835"
     CDS             complement(6150..6575)
                     /locus_tag="PKY13_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80081.1"
                     /translation="MTDTTAARHVLDIHPWDAPHADGGHDLRGEYVERFWLPILGPST
                     LVLVRRIARGLDHAPGGFRIDLELTAAALGLGTAQGEKGPLPRALYRAQTFGFARPVG
                     TNKVAFRTRAATLTVRQLARLPHIVQAAHVGFAEPATVR"
     gene            6714..7151
                     /locus_tag="PKY13_08840"
     CDS             6714..7151
                     /locus_tag="PKY13_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80082.1"
                     /translation="MSPEIDPAIAAALRAREAQDSQSQRAAAEQARYQARLESLHVLS
                     PRAQDMIRQTIRRTKATKVKLPVVRGGSVKLTWLCRGWVLTTYLFDVMDYDSQSMGTR
                     EAPSVALRDNGELRYRNHPMTVGDWETAVGSDDRLAELLATIW"
     gene            complement(7239..8357)
                     /locus_tag="PKY13_08845"
     CDS             complement(7239..8357)
                     /locus_tag="PKY13_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80083.1"
                     /translation="MIAVAAGLGIVSSLLYLPTSSAAAGTDTLYANETLANEQAIRSS
                     NGYTFVMQGDGNAVIYKPTVGGRTAIWSTKTPGRSNSYLAMQSDGNLVLYSNGAAYWA
                     TGMKGAGSYLVMQTDGNLVMYRPSGNSRVAVWSSGTAQASTPTPATTIIVATYNQAGG
                     ADAYPNGYKATFATGTSFIGTQEVCQLRLTSTDPAEGVRDPQPMIRSFGYTKPGCHTS
                     GPGYGNAVRAAWTGAYEPAINRVFAQKDPTGDPRGVVCQRGVISTRWATFCSTHLTAQ
                     AGTAQDNARRNQQTELLAFVGLVRRPGDLIVISGDFNSRPASRMEANTLGLVRATPTD
                     QIDQIWTNATVVRSFSAVPCSPSRCSDHDIWYATLRIR"
BASE COUNT         1376 a         2787 c         2985 g         1371 t
ORIGIN      
        1 cgtaccggtt cgcctgcgac cagttcacga gtcgcgggta ccgcaacacc ctcgacggct
       61 ggcagacgaa catgcgggcc atcgcgttgg ggatggagtc gctgcgacgc ctcgaccgct
      121 acgggatcgc tgccggcggt gagcagtacg tcgggttcga ggcgttgccg gctgggcgcg
      181 caatgcccgc gggcggcgac acgatgacgg tgcaagcggc cgcggagatc ctggcggctg
      241 aggcgatcga cccggcggtg tcggccgtcc cgatcttcac ggcggcggag ctgttgtccc
      301 aaccggtggc acgggtgagc gcgtaccgga tggccgtgcg gcgcctccac cccgaccagg
      361 gcggcgagcc ggccgccttc gtgcggctga acacagcacg cgacgtcctc ggcgagtacg
      421 tgcacagctg agcggccgac gtcggcggga accaccttca accggtgcac agaagcagtg
      481 agggaggcac caccgccgcc cctcaccact tccccaggag aacccccacc atgaagagca
      541 tccgtttccc caagttcatg atcgccgcag ccgtcatcgt cggtgccgcc tcgatcacca
      601 ccgcggcgcc ggcgtcggca tcgacggtca ccggctcgga cgtcaccgtc tcccgcacgg
      661 tgaaggccga ccagtcggcc ccatgtgtcg acccgctgtt ctcgctgtcg aactacgtga
      721 actcgaccgc tgacgccttc accttgacgg tcaacgtcaa cgctccgctc tgctcgccgc
      781 tcgaggcgaa ggccgtgatc tacgccatgc ccggcgacgg tgtcgcctgg ccccaggagc
      841 tcgtcaagac ggtgccgttc acgctcagcg aggccggcac ggtcaccgtc acgttctcga
      901 agggttgcgg cgccacccag ttcgacgtga tcacgggcca cgccccgcag accatcaaca
      961 ccggcttcga tcacgggccg ttgctgttcc ccgcgcagtt gcagaccgcc cagcagttct
     1021 ggggcgctcg gtgtgtcgac gagacgacga ccacatcaac cgtccccgcc accacctcga
     1081 ctgtcccgac atcgtcgacc accactgctg ccacctcgac gtcgaccacg gccgctgagg
     1141 ccaagaccac gacgaccgcg gcctccgccg acgtcaacat cagcgccacc agcacggtcg
     1201 ccgaaccgac gaccacgctc gccatcgcgg tcgccggaca gagcgtcgac aacccggtgc
     1261 cggcaacgag cctggccttc acgggttcgg ccacgaacat catcgccgcc ctcgccatcg
     1321 gcctggtcct gatcggcatt tcggccctcg tcatccggga gcgccgcaac tcgaagtgac
     1381 cggtacctga cctccacacg aagcgccgtc gccccagcgt gacggcgctt cgtcgcgtcc
     1441 gggggagtat cggtcggttc tgccacccac atctctgcca tgtcctcgcc cgcacgccac
     1501 cccaagggca cgccgagctc gctcggcggc cagttccgtt caaccgacca ccacgaaagc
     1561 gacgtcgcac tggccgacgc cggaaccgac gagaggagca ctcgtgagcg cgccgaggca
     1621 gcactctcgt ggttcgcgat gggccaggac acgccaccga cggtcgaact gatcgacgac
     1681 tacgtggcgg tgcggctcga cgagtgcacg atcagcctga cgatcggaat ggacgacggt
     1741 ggtgtcaccg tcacccatct cgacggtgaa gagttcggcc tcgccgagta tctggagccg
     1801 ttcgatcatt ggaccgccga cgagcgcgac ggtgtcggcc tggccgtcca gtcgtgcatg
     1861 tacgacctgg cctcgtgggc caacgctggc gcagcggacg cggtcgcctc cctgaacgca
     1921 tcgctcggcc gcaccgaccc cgaggtgttg gagaagtacg ccttgttggt cgacgagtgg
     1981 gactacagcc acacccggct cgccgagctc gctgacggcg attcgaccgg cgtgtggcgc
     2041 gacgagctcg agcagtcgac cgagcgtggg ttcgacctgc tgcgccggat cgccgcggcg
     2101 acccgggcgg cgcacgacgg tcgcgtctgc tgacgcggtg gtgggcatcg aacgaacccg
     2161 gcgcacatga agtgcatgaa cgctccggtc gacctcaccg cctacgacct catcctgatc
     2221 aacacgagcg ggggcaagga ctcccaggcg atgttgtcct acgtgcacga gctggcgact
     2281 gaagtcggga ttgccgatcg tctggtagca gtgcacgctg atctcggccg agtggaatgg
     2341 ccgggcacag cggagctcgc tgaaacccag gccgcggcgt acggcatccg gttcgtgaag
     2401 gtgtcgcgtc cgcagggcga cctgctcacc cacatcgaac agcgcggcat gttcccctcg
     2461 tcgacgacga ggtactgcac ctccgaccac aaacgcggcc agatccgcaa ggtgatgacg
     2521 gcgctggccg acgagcaccg agctgccggc ggcggtcgga tccggatctt gaactgcata
     2581 gggttgcgcc gcgaagaatc gccggcccgg gcccgccgcg tcgagttcgt cccggagggc
     2641 ccggcgagca acgggcgccg ccgcgtcgac gaatggtcgc cgatcatcga ctggtcgaca
     2701 gccgacgtgt gggacacgat ccggtcgacc gggcttccgc accacccggc ctacgacgcc
     2761 ggcatgggca ggctctcgtg caccttctgc gtactggcgt ctcgatcggc cctggtccga
     2821 gcagcgcagc tcgcgccgga gctggcggcg gagtacgccg cggtggaggc ccggatcggt
     2881 catcgcttca accacaagct gtcgatggcg gagatcatcg ctgcagccgc tgtgtccgaa
     2941 acccccgttg aggtctcggc ctgggtggcc tagccgccgg cgggactgcg aacagcgtcc
     3001 cgagctgtgt cgaagcctgc atagctcgcc aacagcaccc ataggtcacc accgagcggg
     3061 atcgccccgc acggaaggac gaacgggtgt cgcagcgagc cgggttcgat gacggtgacc
     3121 gccacgggtg gaacgccgtg ttctgcgacc ggctcgagcg gatgatgcac ggccgtgctg
     3181 ccgccgatct cgccgccgcc ccaggcgcac tggtgacgct tccgatgttc ctgtgggtcc
     3241 ctggagtcgc cgcaggacgc aaggtcaatg cgaccgggcc gaccggtggt ggcttgcgcc
     3301 tcccggtccg gatgacctgg tcgtgcggcc acgctgtgat tgttggtcgc gacgccccgg
     3361 tcacggtccg gtggaaggcg gcgggcctgg ggatcagcgc atcggggaca acgtcgatca
     3421 ccgctggagc gggcgcaccg gccaaacggc gaacgactgt tggtccggcg gtcgtttgcg
     3481 acgacctcgg cgccccggcc gacatcatcg gcgtgtggcg gaccaagacc gcgaacggtc
     3541 agttcctcgg cgccttggtg tccgacggtc atgcggcgtc gtgggaggcg cgaatgtcga
     3601 tcgaggggta cgtcgatcga gcgtgccgcc tcgccgcaga tttcatcgcc cgcgagatcg
     3661 ccgagttccg cggtgcaccc accgcgacat cggtggtctc cgacatcacg ctcgagaaca
     3721 tcgtgacgcg gatgctggtc ggcacgtcga gcgacggtgc gaccggcgag cgaaccggtt
     3781 cggtcaccaa cttgttggcg aagagccttg agcccgcgac gttttggcgc gtggaccctc
     3841 aacggttcgt gagggtctgg ttgcaacgcg acgccgaatc ggaggtgcgt cgtgcgatcg
     3901 gcgacccgtg gatcgcaccg aaggttcgca aggtggcgac ggcaacagca gccgcctcgt
     3961 ctcgaaggaa gtaccccttc cggcccaaga cacaactcaa gaagaccaag gagctctaca
     4021 acaagaagta cccggcggac cgtctgggcg atcggcgact cgagacagcg ttgacgccgg
     4081 cgtcggaccc gaacgtcgat gccgtggacc tcgagcagtt cgccgaagag accgccgaga
     4141 atcggctcgc cgtatgaggc ccgcaccgcc gcccacaccg ctggcgaacg cgaactcggc
     4201 gctcaggtcc gcgaggacga tgcgcgccga atggtctgtc ggagtcgccg agggcctcct
     4261 cacgagcgtc gacgtcatcg agcacgcgtg caccgtcgag gggcgtccgc tgcgacggat
     4321 gaagctccgc caactgctgt tgagccagcc gcactgggga caggcacgca ccggcgaggc
     4381 gatgacacgg ttcgcccgct tcgccggact ggccccctcg gccgatctcg acgagctgac
     4441 ggtcggctgg ctgatcgaca gccgcgccgg cggccgcagg atctccgcgt ggctcgacgc
     4501 tgtggaggtc cgtcgtgacg gcccgccatg gccggggttc ccctacctac ggaagccggc
     4561 gtagccatgg ataccgacgc gtcgcacgcg gcactgacca tcatccacca gacgactgac
     4621 ccgggacgtc gccgacgcct cgtcgaggac ttccaccgcg aacggatcga cttctacacc
     4681 gctgtcgcct ccaagggtgc ccgtcgttac ggtgcgccca ggtcccagat ccccgacctc
     4741 gcacaggagg tcgggatgct cgccgcgaag atggtggaat cctgggaccc gtccctgatg
     4801 aagttcgagg cgatgctgca cgtggtcgcg tcgcgtcaca ttcgcgagct cgtcaactcg
     4861 ccggcgtggg tcggggtgtc gggagcctcg ctgctgaatc ggcgacgggg tcacctgatc
     4921 gcgacccagc gtcgcctggc ccgtgagcac ggggtggaac cgaccgacga acaggtcgtt
     4981 gaggcgtgga accgcgaagc caagacgcgt ttcggtgatc ctggacgcgc cgggatgatc
     5041 gcgtcgctgt cggatttcac cggggtcagc gtcatgccgc gggagccgca gggcgacagc
     5101 gactcgaccg cttgggcgtc tccgggttcg cccgacatgt tggcggacac gtcggtgttg
     5161 tcggcggagg agtcaccaga gttcgtggct gcggtcatcg aggccgcccg aagcgtcagc
     5221 gacgagctcg gcacggttgc ggaggcgtgg ctgggatcgt ggggcccagg ggagactcga
     5281 cgctgcgccg agatccgcga ggtcgcggcg gccctgggtc agaagtacga gtgggtgcga
     5341 cgccgcgtga accagctgaa ggtgctcgct gttgaggtcg cggtcacgga cttcgggatc
     5401 acctctccat gagcgccggc aaccgcctga cgacgtcggg ggtgagccgg ttgcggagat
     5461 cgcgcaaggt ctgggagatc gccgactcgc tgacgccgta gtggtcggcg atcgccttct
     5521 gggaccagga cttgtcggcg gtgatgagct gcagcacccg gaggtggcgt tcggtgacca
     5581 gcccggggat ggaccggagc tggtcgagta tctcatcggc ggagtacccc tcgacggctc
     5641 cttcggggtc gccgtcggtg gcgacctgct cggcgccggg ttcggctggc ggctcgtcgc
     5701 gatacagggc ccggagggca tcgagggccg cgtagtggcc cttgaagtgg caccagccga
     5761 ccagtgaccg ctgctccggg tcccacgatt cgacggctct ccacgctgcg atggcggcga
     5821 cttggcggat gtcgtctcgg taccgtgccg gggtgaggct gacgagccgt ctgaccgcgt
     5881 aggtccgatc gacggtttcc catggccacc ggtcggtgaa ctcgtcgagg agtcgacgtt
     5941 tctcgtcgcc gatgtcgagt ccgtcgagca cggcgaggtc gcgccctcga cgtgcggcga
     6001 gtgtgttgtg ttgcacacgg tgaacatgcg tagaagcagg tccccgggcg ggggttgggc
     6061 cgctttggcc gggtgttagt cgtcaacgat gcgacgccgc tccgcaggat ccatggacgg
     6121 gtcggcaacg tgggggctcg ccactgtgct catcgcaccg tggccggctc cgcgaacccc
     6181 acatgggcgg cctggacgat gtgggggagc cgcgccaact gccggacggt cagcgtcgca
     6241 gcccgggtac ggaacgccac cttgttggtg ccgacggggc gggcgaatcc gaacgtctgg
     6301 gcacgataga gggcacgcgg cagcggcccc ttctcgccct gggcggtgcc gaggcccagg
     6361 gcagcggcag tgagctcgag gtcgattcgg aacccgccgg gtgcgtggtc gaggccgcgg
     6421 gcgattcggc gcacgaggac cagtgttgag gggccgagga tcggcagcca gaaccgttcg
     6481 acatactcgc cgcgaaggtc gtggccaccg tcagcgtgag gcgcgtccca cggatggatg
     6541 tcgagcacat ggcgcgcagc agtggtgtcg gtcatgccaa ctatgtggcc cggtcccggg
     6601 cgcttcccgc catcgagccg gcggggtggg gatcgcgccg gcggcgtcct ggccgctgtg
     6661 gccgggcacg acacgtcctc gacggcgcgg gagcgcgaag ctaccgtctg gccatgtcgc
     6721 ctgagatcga tccagcgatt gcagcagcgt tacgagctcg tgaggcacag gattcgcaga
     6781 gccagcgtgc cgccgcggaa caggctcgct accaagcacg gctggagagc ctgcatgtgc
     6841 tgtcccctcg agcccaggac atgatccgcc agacgattcg tcggacgaag gcaacgaagg
     6901 tgaagttgcc cgttgtgcgt ggggggtccg taaagctcac gtggttgtgt cgaggatggg
     6961 tcctgaccac gtacctgttc gacgtgatgg actacgactc tcagtcgatg ggtacccgcg
     7021 aggccccctc ggtcgccctg cgggacaacg gcgaactccg ctaccgaaac cacccgatga
     7081 ccgtcggtga ctgggagacc gctgtcggat cggacgaccg tctcgctgag ttgctggcca
     7141 cgatctggta gagcgctgac ccacgcctca accgggtctg gtcggtgagg gccggccggg
     7201 tgcagatgtc ttcggctcaa gcccggcgaa gggcggggtt atcggatgcg cagggtggcg
     7261 taccagatgt cgtgatccga gcatcgagag ggcgagcacg gaaccgctga aaaggatcgg
     7321 acaacggtgg cgttcgtcca gatctggtcg atctggtcgg tcggcgtcgc tcgaacgagc
     7381 ccgagcgtgt tcgcctccat gcgcgaggct ggcctcgagt tgaaatcgcc agagatcaca
     7441 atgagatcgc cggggcggcg gaccaggccg acaaacgcga gcagctcggt ctgttgattc
     7501 cgccgagcat tgtcttgcgc ggtacctgct tgtgcagtga ggtgcgtcga gcagaaggtc
     7561 gcccagcggg tggagatcac accccgttga cagacaaccc cgcgggggtc acccgtcgga
     7621 tccttctggg caaagaccct gttgatcgcc ggttcgtagg cgccggtcca ggctgctcgt
     7681 accgcattgc cgtatccggg gccactcgtg tggcagcccg gcttggtgta gccaaatgat
     7741 cggatcatcg gctgtgggtc tcggacacct tcagcggggt cggtgctggt gagtcgtagt
     7801 tggcagacct cttgagtgcc gatgaacgat gtccccgtcg cgaaggtcgc cttgtagccg
     7861 ttcgggtagg cgtcggcgcc gccggcctgg ttgtaggtgg caacgatgat cgtcgtcgct
     7921 ggcgtcggtg ttgatgcctg ggcggtgccg ctcgaccaga ctgcgacgcg gctgttccct
     7981 gatgggcggt acatgacgag gttgccgtcg gtctgcatca cgaggtacga cccggccccc
     8041 ttcatgccgg tcgcccagta cgcggcgccg ttggagtaga gcacgagatt cccgtcgctc
     8101 tgcatcgcca ggtaggagtt ggagcgtccc ggcgtcttgg ttgaccagat cgcggtgcgc
     8161 ccgccgacgg tcggcttgta gatgacggcg ttgccgtcgc cctgcatgac gaaggtgtag
     8221 ccgttggagg agcggatggc ttgctcgttg gccagcgtct cgttggcgta gagggtgtcg
     8281 gtgccggctg cggcgctcga ggtgggcagg tagaggagcg acgagacgat gccgaggcct
     8341 gcggccactg cgatcactcg cctgaacgga cttttgatca tggccacccc aatgctgttg
     8401 gtcgtcgcgg tgctgaacaa catccaccgc cgatgaccgg aagctagttc ggcgacagct
     8461 ggtcgagaag ggcgcaggtc actttggggc aggactgctc acgggcgggg cggggggtg
//
LOCUS       DAOSOA010000118         8497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8289_length_8497_cov_5.040986, whole genome shotgun sequence.
ACCESSION   DAOSOA010000118 DAOSOA010000000
VERSION     DAOSOA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8289_length_8497_cov_5.040986"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..581)
                     /locus_tag="PKY13_08850"
     CDS             complement(117..581)
                     /locus_tag="PKY13_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80084.1"
                     /translation="MFRCKPLAVAVFAMWTLLVWANRIYNIVEKDGGDGVDLARAIGF
                     VVVGLVVAVVAVRPMPAPLAHRLVSVAGWASIALWATRIVTIPLGDYSVGFVVVHLVL
                     AAISISLALWAMRSTRSLAAVDGASLGDPDATSDHHRPSTGTDVVDPATTRA"
     gene            700..1770
                     /locus_tag="PKY13_08855"
     CDS             700..1770
                     /locus_tag="PKY13_08855"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOT80085.1"
                     /translation="MSTLPPPDVPAIFWIDGAGQQQGPEPLATVIERIAHDQIPSTTP
                     VWWQGAVNWSPFNSNAELAAALATRLAPAPAPAPAPEPQVATQSPFAATTSSPFSSPA
                     PSESTSVFGSPASFEQPVTFEAPVTSPFEAPATIEIPAAAATFDAAAPVVADPTPTFD
                     TASAPVTYDAPTTGFDAPAAADAPVAVEIPVAEAAPVVSDVPEVIVPDEPVVAAPSLQ
                     LGDAAELESTFASLTERSSAFDAEVARAAALDNTVAGLVRQALGELGFSIDESLTSDD
                     YHSFRLSEPGGAVASLAIQRVPAAIDVAAATERPIACFVDRAGRASVGLFVGDYLTDD
                     GTDQALFTKHLASVVNAAGTAS"
     gene            complement(1820..2611)
                     /locus_tag="PKY13_08860"
     CDS             complement(1820..2611)
                     /locus_tag="PKY13_08860"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT80086.1"
                     /translation="MSSRTTRPTAQKRPKVAAPLDNASRAVRRLLAAEGDVAVERVTE
                     QIPAIAPQGTGTERPLPEWVELNQSLYRVFAFVDVCGFTKYTDQFGAHAATEMLSRFR
                     SACRDVTARRGARVAKWLGDGVMLVGTEPGPVIATVAELLLRFRDDAFEIHAGIAAGT
                     VLLFEGDDYIGRSVNLAARLCEAAAPGELLAHAVDDAVPDWVNLAGTVTVRAMGIGDV
                     AGVSQLRVDDQAWATMSPVVSHPSLALVSTEFSPLPDERSDDPTD"
     gene            complement(2671..3435)
                     /locus_tag="PKY13_08865"
     CDS             complement(2671..3435)
                     /locus_tag="PKY13_08865"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT80087.1"
                     /translation="MAPRRPPNLGVERTFWDAGVEYVVGIDEVGRGSWAGPLTIGAAI
                     VPRDRRVNRIRDSKQLTERNREALFDRIAGWCEAWAVGHASPQECDDLGMSEAQRVAT
                     RRALAALGIEPGVALIDGKWDFVGGGPTGRTVTRTIVKGDATSLSIASASILAKVSRD
                     RIMRELADEFPAYSFETNKGYPCPRHKTALQGYGPSSIHRRSWVFMDHLVWGGLHRPV
                     ADLPEGDQLALDLTERSDGEPMPLPDGLDELEISRW"
     gene            3556..3816
                     /locus_tag="PKY13_08870"
     CDS             3556..3816
                     /locus_tag="PKY13_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80088.1"
                     /translation="MQYNANDTERADRARELVEVITIALGTSVEVRRRFDQAWARGFE
                     VASATEEGYRLRRQSDGAVLPVSFPPDDVRRSSGSPTIHLPR"
     gene            3944..4774
                     /locus_tag="PKY13_08875"
     CDS             3944..4774
                     /locus_tag="PKY13_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOT80089.1"
                     /translation="MTTEARSPRRGRPRRAERGAILILTAVSMVGMMCFAGLAIDVGM
                     VRSQRAQYQAAADAAALYATFLIRQPGADVSTVAAQVRTFVAQNLDIADGTADSTWAH
                     CADAKKLTTVSPLDASIGNDCISFVLSGSASQARVRIPMRRVKPVIGLGLATMEVTAV
                     AGAEGSGAGCDAYAVAGCATPTTAAPTTTSTTIKATTTTTRPGPTTTVKPTTTIYGTT
                     TTTKKPVTTTTRKPTTTTTVKPGPTTTTIKATTTTAKPTTTVTTTTAPPVPTTLDIGF
                     "
     gene            complement(4797..5804)
                     /locus_tag="PKY13_08880"
     CDS             complement(4797..5804)
                     /locus_tag="PKY13_08880"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOT80090.1"
                     /translation="MRITVGIIGGGSWGTTVGHLAAHNVSTVLWARHAETAEEINSHH
                     RNSRYLDGFDLHPALRATADLGEAVRQADVLVMGVPSQSFRSTLEEVSDHLRAWVPIV
                     SLAKGLEQGTRLRMSEVAHQVVPGHPVAVLTGPNLAKEILAGDAAASVLATHDPVVAA
                     RLQEVFSTNLFRVYTNPDVVGCEVAGALKNVIAIASGMADGLGAGDNTRAAVITRGLA
                     ELSRLGVAMGGNELTFAGLAGMGDLLATCISPQSRNRHVGEQLGRGRSIDEVVAEMNM
                     VAEGVKTAPVVLDIGAEFGIDVPISSQVYAVCHQGRPAAEAYEGLLRKGSGVEHPNLD
                     F"
     gene            5928..6578
                     /locus_tag="PKY13_08885"
     CDS             5928..6578
                     /locus_tag="PKY13_08885"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOT80091.1"
                     /translation="MTTDRTSIRRRPTRRPARRGRGERGAALIEFALAFPILFLLMMG
                     VVDFGVNYGNKVETSHAAREGARAGSVGRVGTDTSCYVARTPPSTEARQLACLVKART
                     HMDSEDVRVRFTYMDGDGRYTNDFSEAKRQANRYSIMVCVSTRAFSLSGLLAPVFNGK
                     FHHSRAVIKTGTTPWSNVAASGATVYSYVPQYAETTFAGDSWSWCTSDDPAADINL"
     gene            6584..7192
                     /locus_tag="PKY13_08890"
     CDS             6584..7192
                     /locus_tag="PKY13_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOT80092.1"
                     /translation="MTLSHLLHRRRWARLDGRTERGAVLVELAMVVPILVVLIMGIVD
                     YAVLFNQKIGIRGGVREAAWNGSRGIFGAAPNSSCSLTFPAALPDDNTQRIMCMVKRR
                     SGLDQSELRVMVRVVDLDNPANPATYANGKGLMVCAMRKASSATHFFSSLFATSYQVS
                     RLTTVILSIDPNVTFVGGGSETPFPTKAGGWSFCDPTRPVPA"
     gene            7217..7825
                     /gene="cofC"
                     /locus_tag="PKY13_08895"
     CDS             7217..7825
                     /gene="cofC"
                     /locus_tag="PKY13_08895"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HOT80093.1"
                     /translation="MPAGCLVLVPLRSFLDPKTRLADVLTLPERADLARRLAGDVLDA
                     LDGWDVAVLTDDDDVIRWADQRDVTVARPGIAGLNAAVTAGAELAVDRRAEVIAVVHA
                     DLAMPDALTDVLTSATAPERAGGVTAVPDRHGDGTNVLVVPAGAGFRFGYGPGSFGRH
                     RDEATRLGLAFVEVAHAGLGWDVDTPSDLSAYLTLGDPTRGG"
     gene            7972..>8497
                     /locus_tag="PKY13_08900"
     CDS             7972..>8497
                     /locus_tag="PKY13_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HOT80094.1"
                     /translation="MLTSIVMIVLMAFCAFAVDIGQRNQQLAGAQHAIDAAVITAAQY
                     LSEHDGDYAGASARVKEIIRQNLGIDPASWPACDDPNHLDVSAPNGHDLHQLPAHRRD
                     RDDPGQERHPGSAPLLPDGHDLRVGAGARLDRPGSDRCLQREQLRRRDQPGVRAGHEC
                     GDHDHHSAALAHHHD"
BASE COUNT         1344 a         3134 c         2821 g         1198 t
ORIGIN      
        1 gcaccgccca ggacgtcgcc caggccgccg agcccaccag cggggtcacc ggcaccgcgc
       61 tgatcgatca tgtcggagga cttcgcctcg tccttgtccc attccatgga gcctcctcag
      121 gcgcgcgtcg tcgccggatc gacgacatcg gtaccggtgg agggccggtg atggtcggag
      181 gtggcgtcgg ggtcaccaag agaggcaccg tcgacggcgg ccagcgaccg cgtcgaacgc
      241 atggcccaga gggccaacga gatcgagatg gcggcgagca cgaggtgcac gacgacgaag
      301 ccgaccgagt agtcgccgag cgggatggtg acgatgcgag tggcccacag cgcgatcgag
      361 gcccagccgg ccaccgacac caaccggtga gcgagcggcg ccggcatcgg tcgcacggca
      421 acgacggcga ccaccagtcc gacgacgacg aacccgatgg cgcgagccag gtcgaccccg
      481 tcgccaccgt ccttttcgac gatgttgtag atgcggttgg cccagacgag cagggtccac
      541 atcgcgaaca cggcgaccgc caacggtttg caacggaaca tgagccggga ggttagttgc
      601 cgaccccgcg tcgccgtcca tcggtgggag gatcacggcg cgcgtaaaaa agcccacgga
      661 aggatttacc cggcggaagt gctgtgggag gctctcggca tgagcacgct tccccctccc
      721 gatgtgcccg ccatcttctg gatcgacggc gccggccagc agcagggccc cgagcccctc
      781 gccacggtga tcgagcgcat cgcccacgac cagatcccgt ccacgacccc ggtctggtgg
      841 cagggcgcgg tgaactggtc gccgttcaac tcgaacgccg agttggccgc ggcgctcgcc
      901 actcgcctgg cacccgcgcc ggcacccgcc cctgcgcccg agccccaggt cgcgacccag
      961 tcgccgttcg cggcgaccac gtcgtcaccg ttcagctccc cggcaccgtc ggaatccacg
     1021 tcggtcttcg ggtcgcccgc ctcgttcgaa cagccggtca ccttcgaggc cccggtgacg
     1081 tcgccgttcg aggcacccgc cacgatcgag atcccggcgg cggcagcgac gttcgacgcg
     1141 gcggcgccgg tggtcgccga ccccaccccc accttcgaca ctgcctcggc accggtcacc
     1201 tacgacgctc ccaccaccgg cttcgacgct ccggcggccg ccgacgctcc tgtggccgtc
     1261 gagatccccg tcgccgaggc cgcgccggtc gtgtccgacg tccccgaggt catcgtgccc
     1321 gacgagcccg tcgtcgccgc tccgtcgctc cagctcggcg acgccgccga actcgagtcg
     1381 acgttcgcgt cgctcacgga gcggtcctcg gccttcgacg cggaggtcgc ccgggccgca
     1441 gcgctcgaca acaccgtcgc cggcctcgtg cgccaagcgc tgggcgagct cggcttcagt
     1501 atcgacgagt cgctcacgtc cgacgactac cacagcttcc gtctgtccga gcccggagga
     1561 gcggtcgcct ccctcgccat ccagcgggtc cctgccgcca tcgacgtcgc tgccgcgacc
     1621 gagcgcccga tcgcgtgctt cgtggaccgc gccggacggg catcggtcgg cctcttcgtc
     1681 ggcgactacc tcaccgacga cggcaccgac caggcgctct tcaccaagca cctggcgagc
     1741 gtcgtcaacg ccgccggcac ggcgagctga atcggtcggc cgttcgctgc gccgccgggc
     1801 ccggcggctg acggcgacgt cagtcggtgg ggtcgtcgga gcgttcgtcg gggagcgggc
     1861 tgaactccgt cgacacgagt gcgagcgacg gatggctcac gaccggtgac atcgtcgccc
     1921 acgcctggtc gtcgacgcgg agctgactga cgccggccac atcgccgatg cccatggccc
     1981 gcaccgtgac ggtgccggcg aggttgaccc agtcgggaac agcgtcgtcg accgcgtgcg
     2041 ccaacaattc gccgggagcg gcggcctcgc acaggcgggc ggccaggttg accgaacggc
     2101 cgatgtagtc atcgccctcg aacagcagca cggtgcctgc ggcgatgccg gcgtggatct
     2161 cgaacgcgtc gtcgcggaac cgcaggagca actcggcgac cgtcgcgatg accggcccgg
     2221 gctcggtgcc gacgagcatg acgccgtcgc ccaaccactt ggccactcgt gcgccacggc
     2281 gtgccgtgac gtcgcggcac gccgagcgga accgcgacaa catctcggtg gcggcgtgcg
     2341 ccccgaactg gtcggtgtac ttggtgaatc cgcacacgtc gacgaaggcg aacacccgat
     2401 agagcgactg gttgagttcg acccactccg gcagcgggcg ctcggtgccc gtgccctgcg
     2461 gagcgatcgc cgggatctgc tcggtgaccc gctcgaccgc cacatcaccc tcggccgcga
     2521 ggagccgccg gacagcgcgg gaggcgttgt cgagcggggc agcgaccttc ggacgcttct
     2581 gggcggtcgg gcgtgtggtt cggctgctca tgtcgagttc gatcgtccca ggtagtgctt
     2641 gctgttgcaa gcatacccag ggtgacatct ttaccaacgc gagatttcga gttcgtcgag
     2701 gccgtcgggc agcggcatcg gctcgccgtc ggagcgttcg gtgagatcga gcgccagctg
     2761 gtcgccctcg gggaggtcgg cgaccggccg gtgcaggcct ccccacacga ggtgatccat
     2821 gaacacccag gaccgccggt ggatcgacga cgggccgtac ccctgcagag cggtcttgtg
     2881 ccgggggcag gggtacccct tgttggtctc gaagctgtac gccgggaact cgtcggcgag
     2941 ctcccgcatg atgcggtcgc ggctgacctt ggcgaggatc gacgccgagg cgatcgacag
     3001 cgacgtcgcg tcgcccttca cgatcgtgcg ggtcacggtc cggccggtcg gcccaccgcc
     3061 gacgaaatcc cacttgccgt cgatcagcgc aacaccgggt tcgatgccga gcgccgcgag
     3121 ggcacggcgg gtggcgaccc gttgggcttc ggacatgccg aggtcgtcgc actcctgcgg
     3181 ggaggcgtgg ccgacggccc acgcctcgca ccagccggcg atgcgatcga acagggcctc
     3241 gcgattgcgt tcggtgagct gtttggagtc tcggatgcgg ttgacccgtc ggtcgcgggg
     3301 cacgatcgcc gcgccgatcg tgagcggccc ggcccacgag ccgcggccga cctcgtcgat
     3361 ccccaccacg tactcgacgc ccgcatccca gaacgtgcgc tcgacgccca ggttcggtgg
     3421 gcgtcgaggg gccatggccc ggagcgtacc gcgtggtccg tcgcgttgac tcgcccccca
     3481 ggggcggtat cctgaacgca cacgggggtc acagcaccac acaaagtggt cgttggcgag
     3541 actcgagggg gacgaatgca gtacaacgcg aacgacaccg agcgagccga ccgagcacgc
     3601 gagctcgtcg aggtgatcac catcgccctc gggacgagcg tcgaggtgcg ccgacggttc
     3661 gaccaggcct gggcccgcgg cttcgaggtc gcgtccgcga ccgaggaggg ctaccggctc
     3721 cgccgacagt ccgacggagc ggtcctcccc gtgagcttcc cgcccgacga cgtccggcgg
     3781 tcgtcgggct cccccaccat ccacctcccc cgctgatctc gacaccggcc ggagcggcgc
     3841 gtcagcgcca cgctcaccgg agggccggtg ggcccaccgc accaacgaga acccgcccaa
     3901 cggcccattg cccgaagcgg cgtatgacgt cacggtgtac ctcgtgacca ccgaagcccg
     3961 ctccccacgc cgaggccgcc cccgccgcgc cgaacggggc gcgatcctga tcctgaccgc
     4021 cgtgtcgatg gtcgggatga tgtgcttcgc cggcctcgcg atcgacgtcg gcatggtgcg
     4081 gtcccagcgg gcgcagtacc aggcagctgc cgacgccgcc gcgctctacg ccacgttcct
     4141 gatccgtcaa ccgggcgccg acgtctcgac cgtcgccgcc caggtccgaa ccttcgtggc
     4201 acagaacctc gacatcgccg acggcacggc cgactcgacc tgggcccact gcgccgacgc
     4261 caagaagctc accaccgtga gcccgctcga cgcctcgatc ggcaacgact gcatcagctt
     4321 cgtgctgagc ggctccgcca gccaggcccg ggtccggatc ccgatgcgtc gggtgaaacc
     4381 ggtcatcggg ctcgggctcg cgaccatgga ggtcacagcg gtcgccggcg ccgagggcag
     4441 cggggccgga tgcgacgcct acgcggtcgc cgggtgtgcc acccccacga ccgctgcacc
     4501 gacgaccacg tccaccacga tcaaggcgac gacgaccacg acgaggcccg ggccgaccac
     4561 caccgtcaag ccgaccacga ccatctacgg cacgacgacg accaccaaga agccggtgac
     4621 cacgacgacc cgcaagccca ccacgaccac gacggtgaag cccggtccga cgaccacaac
     4681 gatcaaggcg accacgacca cggcgaagcc caccacgacc gtgacgacca cgaccgcgcc
     4741 gccggtcccg acgaccctcg acatcgggtt ctaggaccgg cgcagtgtct cgctcgtcag
     4801 aagtcgaggt tggggtgttc gacccccgag cccttccgga ggagaccctc gtaggcctcc
     4861 gctgccgggc ggccctggtg gcacacggcg tacacctggc tcgagatcgg cacgtcgatg
     4921 ccgaactcgg cgccgatgtc gagcacgacc ggcgcggtct tgaccccttc ggcgaccatg
     4981 ttcatctcgg cgacgacctc gtcgatggaa cgacctcgac cgagctgctc gccgacgtgg
     5041 cggttgcggc tctgcggcga gatgcacgtg gcgagcagat cgcccatgcc ggccagtccg
     5101 gcgaacgtga gctcgttccc gcccattgcg acgccgaggc ggctcagctc ggccagccca
     5161 cgggtgatga ccgcggcacg ggtgttgtcg ccagcaccga gcccgtcggc catgcccgac
     5221 gcgatggcga tcacgttctt gagcgccccc gcgacctcgc aaccgacgac atcggggttc
     5281 gtgtagacgc ggaacaggtt cgtggagaac acctcctgca gccgggcggc cacgaccggg
     5341 tcgtgggtgg cgagcaccga cgccgcggcg tcgccggcca ggatctcctt ggccaggttg
     5401 gggcccgtga gaacagcgac gggatgacca gggacgacct ggtgggcgac ctcgctcatc
     5461 cgcagtcggg tgccctgctc gagccctttc gcgaggctca cgatcggcac ccacgcccgc
     5521 aggtggtcgg acacttcctc gagcgtcgag cggaacgact gggacggtac gcccatcacg
     5581 agcacgtcgg cctgacggac ggcctcaccg aggtcggccg tggcccgcag cgccggatgc
     5641 aggtcgaagc cgtcgaggta ccggctgttg cggtggtggc tgttgatctc ctcggcggtc
     5701 tccgcgtgcc gcgcccacag gaccgtggag acgttgtggg cagccaggtg gcccaccgtc
     5761 gttccccacg aaccgccacc gatgatgcca accgtgatgc gcatgctgtg accctattgc
     5821 gttgcgaccg ctgcggagcg gccgaaccgg ggactgagcc gatcggcccg gcggctgtgg
     5881 gaaaagtgcc cggtcggatc ctgcgtccga gcctgttcga tggatgcgtg accaccgatc
     5941 gcacatcgat ccggcggcgc cccacccggc gtccggcccg acgggggcgc ggcgagcgcg
     6001 gtgccgcgct gatcgagttc gcgctggcct tcccgatcct cttcctcctg atgatggggg
     6061 tcgtcgactt cggggtgaac tacggcaaca aggtcgagac cagccacgcg gcccgcgagg
     6121 gcgctcgagc cgggtcggtc ggacgcgtcg gtaccgacac ctcgtgctac gtcgcccgga
     6181 ccccaccgtc gaccgaggcg cggcagctcg cctgcctcgt caaggcccgc acccacatgg
     6241 acagcgagga cgttcgcgtg cgattcacct acatggacgg tgacggtcgc tacacgaacg
     6301 acttctccga agccaagcgg caggccaacc gctactcgat catggtgtgc gtctcgacca
     6361 gggcgttctc cctgagcggc ctcctcgcac cggtcttcaa cggcaagttc caccactccc
     6421 gggccgtgat caagaccggt accacaccct ggtcgaacgt cgcagcgagc ggcgccaccg
     6481 tctactccta cgtcccgcag tacgccgaga ccacgttcgc cggcgacagc tggtcgtggt
     6541 gcacgtccga cgaccctgcc gccgacatca acctgtgacc accatgacgc tctcccacct
     6601 cctccaccgg cgccgctggg cccgactcga cggtcgcacc gaacgcggcg ccgtgctggt
     6661 cgagctcgca atggtggtgc cgatcctcgt ggtcctcatc atgggcatcg tcgactacgc
     6721 cgtgctcttc aaccagaaga tcggcatccg tggtggtgtc cgcgaggcgg catggaacgg
     6781 cagccgtggc atcttcggcg ccgcgcccaa ctcgtcgtgt tcgctgacgt tcccagcggc
     6841 gttgcccgac gacaacaccc agcgcatcat gtgcatggtc aagcgccgca gcggcctgga
     6901 ccagtccgag ctgcgggtca tggtgcgagt cgtcgacctg gacaaccccg ccaacccggc
     6961 gacctacgcc aacggcaagg gcctgatggt ctgtgcaatg cgcaaggcgt cgtcggcgac
     7021 ccacttcttc tcgtcgctgt tcgccacgag ctaccaggtg tcgaggctga ccaccgtcat
     7081 cctctcgatc gacccgaacg tcacgttcgt cggcggtggc agcgagacac cgttccccac
     7141 gaaggccggc ggctggtcgt tctgcgatcc caccaggccg gtcccggcct aggggccacg
     7201 ccgtagagtc gggcccatgc ccgccggttg cctcgtgctc gtccccctgc gctccttcct
     7261 cgatccgaag acccgcctcg ccgacgtgct caccctgccg gaacgggccg acctggcgcg
     7321 acgactggcc ggcgatgtcc tcgatgccct cgacggatgg gacgtcgcgg tgctcaccga
     7381 cgacgatgac gtgatcaggt gggcggacca acgcgacgtc accgttgcgc gacccggcat
     7441 cgccgggctg aacgctgccg tcaccgccgg cgccgaactc gccgtcgacc gacgggcgga
     7501 ggtgatcgcc gtcgtccacg ccgacctcgc catgcccgat gccctcaccg acgtgctcac
     7561 atccgcaaca gcgcccgagc gggccggcgg ggtgaccgcc gtacccgacc gccacggcga
     7621 cgggaccaac gtgctcgtcg tcccggccgg tgccgggttc cgcttcgggt acggaccggg
     7681 ttcgttcggc cgccatcgag acgaagcgac ccggctcggc ctcgcgttcg tcgaggtcgc
     7741 ccacgccggg ctcggctggg acgtcgacac cccgagcgac ctctcggcct acctcaccct
     7801 gggtgatccg acccggggag ggtgagccga ccaggcccga gaacgagaga tcaacctgtc
     7861 acagcaggcg gctctcctgc caatagagac aggatgcgtc cgacactcct ccgttccgca
     7921 agcggccggc cgcggcggcg ccgcgagcgt ggcgatcgag gggccatcct cgtgctcacg
     7981 agcatcgtga tgatcgtcct catggcgttc tgcgcattcg cggtcgatat cggccagcgg
     8041 aaccagcaac tcgccggcgc tcagcacgcc atcgacgctg cggtgatcac cgcagcgcag
     8101 tacctgagcg aacacgacgg cgactatgcc ggtgcgtcgg cgcgggtcaa ggagatcatc
     8161 cgccagaatc tcggcatcga cccggcgagc tggcccgcct gcgacgaccc caaccacctc
     8221 gacgtgtccg cgccgaacgg acacgacctg catcagcttc cggcgcatcg ccgcgaccgc
     8281 gacgacccag gtcaagaacg acatccgggt tcggctcccc tcctaccgga tggacacgat
     8341 cttcgggtcg gcgctggggc tcgactcgat cgacctggca gcgaccgctg cctccaacgg
     8401 gaacaactgc ggcgccggga ccagccaggc gtgcgggccg ggcacgagtg cggcgaccac
     8461 gaccaccaca gcgccgccct cgcccaccac cacgacg
//
LOCUS       DAOSOA010000119         8417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8393_length_8417_cov_8.260464, whole genome shotgun sequence.
ACCESSION   DAOSOA010000119 DAOSOA010000000
VERSION     DAOSOA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8393_length_8417_cov_8.260464"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKY13_08905"
     CDS             <1..663
                     /locus_tag="PKY13_08905"
                     /inference="COORDINATES: protein motif:HMM:NF018436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HOT80095.1"
                     /translation="EGAIEWIAGQPFGKFLLVLMTGALFSLAAWRILDAVFGDPVEGD
                     SASDRARFAAKAAVYLGFAIASLSAMLANWSGTSGSGSGSSDGQTQKEATAFVIGWPM
                     GQWIVGAVGLGVIGYAVYMFKHHAIDERFMKRLSADLTSVRTLGRAGYGARSVVWAVV
                     GGLLIQAAVTYDPNKAGGLSAALQRLAQTTGGPVLLALVALGLFAFAAFCVAEAKYRR
                     AA"
     gene            832..905
                     /locus_tag="PKY13_08910"
     tRNA            832..905
                     /locus_tag="PKY13_08910"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Lys,seq:ttt)
     gene            complement(930..1568)
                     /gene="vanX"
                     /locus_tag="PKY13_08915"
     CDS             complement(930..1568)
                     /gene="vanX"
                     /locus_tag="PKY13_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HOT80096.1"
                     /translation="MSGPWLFVDEAVPGVRCDAKYATWDNFTGRPVDGYAANRVVGTQ
                     ALCAALLLARDVAGSLGFGLLVWDGYRPQRAVDAFARWSEQPEDGRAKDRHYPNIERT
                     EMFDLGYIAARSGHSRGSTVDLTLFHLDSGELAAMGGHHDLMDPVSHHGATGITSVET
                     SNRLALRTVMEVSGFDAYEFEWWHYTLRDEPFPDTYFDFPIESVPGRFQSVD"
     gene            complement(1565..2614)
                     /locus_tag="PKY13_08920"
     CDS             complement(1565..2614)
                     /locus_tag="PKY13_08920"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOT80097.1"
                     /translation="MDRTRIGIVFGGCSEEHAVSVKSAREVARHLDRARFEPVFLGIT
                     LDGAWSWCDGPEDGWELRGRPVVLSPDRTVHGLLVPGQHPDRYESLRLDVVFPVLHGT
                     SGEDGAIQGLLELAGIPYVGCDIQSSALCMDKSLAYVICSAAGISTPTFRVVTREDVG
                     VGDLSGLEYPLYVKPARSGSSFGVTRVEHGGELSEALAAARTYDSKVVIEQAVVGTEV
                     GCAVLGRGTELSVGCLDRISLSHGLFRIHQEDDPEHGSENSTVTVPADIPEETAQLVR
                     ETALAIYRALGCRGLARVDMFVTPDGDVVLNEVNTLPGLTSYSRYPRMMAAAGWSLGD
                     VIERLVALAMADGSS"
     gene            complement(2571..3566)
                     /locus_tag="PKY13_08925"
     CDS             complement(2571..3566)
                     /locus_tag="PKY13_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT80098.1"
                     /translation="MSGVTVFGCRPDVLGVVEREALRLGLSATVTAAAPSIRHLELVA
                     GNRCISIGHDCVVGPELLAALRASGVTYVSTRSAGTDHIDVLDARHHGIVVENVEYSA
                     DSVADHTLMLILMAVRHARTTVGRADAGDYRLPETPGRELRDLTIGIVGTGRIGSAVI
                     DRLRGFGCRLLGHDRSASGSVPHVPLDELLRESDVVTLHTPLTIDTHHLLDRSRLALM
                     KPGAFLVNTGRGALVENEALIAALETGHLAGAALDVIEGERGTFYADCSGVRDDQSAI
                     ARLQRLPNVVITPHVAFYTDRALAEMFVTSLRNCARFEGRRYGSNEDRHRVRGVL"
     gene            complement(3563..4114)
                     /locus_tag="PKY13_08930"
     CDS             complement(3563..4114)
                     /locus_tag="PKY13_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT80099.1"
                     /translation="MSSTRSVARSATRPTIAVVLVGVVVCLVAWQWSGTSSERVVPDG
                     TTVFDLDVPAVSRLAPDLLDALRRATTDADRDGVVLDIKSGWRSSAEQADLLADAVTR
                     YGSAEIAARWVAPPSRSAHVSGDAVDVGGHGASAWLSEHGVAYGLCRIYDNEPWHFEL
                     RSAAVSDGCPPRYPDASHDPRMR"
     gene            4201..4932
                     /locus_tag="PKY13_08935"
     CDS             4201..4932
                     /locus_tag="PKY13_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT80100.1"
                     /translation="MRVLIVEDEPLLADAIRDGLRLEAIAADVAGDGDTALALLDVNT
                     YDIAVLDRDIPGPSGDEVAARIVASGSGMPILMLTAASGLDDKVSGFELGADDYLTKP
                     FELRELVLRLRALDRRRADPRPPIRELAGLRLDPFRREVYRDGRYVALTRKQFAVLEV
                     LVDASGGVISAEELLERAWDAHADPFTNAVRITVSALRKRLGEPWLIVTVPGVGYRID
                     ATPVGEVPETGGTDGSGADDGGERA"
     gene            4958..>5662
                     /locus_tag="PKY13_08940"
     CDS             4958..>5662
                     /locus_tag="PKY13_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT80101.1"
                     /translation="MSVRLKLTLSYAGFLMVAGVLLLATVWVFLLRYVPARIDAVSSE
                     SSPGSPSTFVPSRVDLLNAFAPKAAWALAFLLVFGLVGGWLLAGSMLAPLRRITGVAR
                     QAGAGSLSQRVQMEGRNDEFRELADTFDTMLARLEAHVAEQQRFAANASHELRTPLAI
                     TQALLQVAREDPTHDADELVKRLIHVNSRAIDLTEALLLFSRAEQGVMSREPVDLSLL
                     AEDAVETLLPLAEDRGI"
     assembly_gap    5663..5762
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5763..6237
                     /locus_tag="PKY13_08945"
     CDS             <5763..6237
                     /locus_tag="PKY13_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT80102.1"
                     /translation="LAEDAVETLLPLAEDRGIAIENSGEVATTSGSRTLLQQVTTNLV
                     HNAIVHNVPASDRDEAGSVWITTSPRADWAALTVENTGDVLTPEHVATLVEPFNRGAA
                     RVRTEHTGVGLGLAIVDTIVDAHGGRLELTPRRGGGLRATVLLPLLTPSSGTAAV"
     gene            complement(6248..7192)
                     /locus_tag="PKY13_08950"
     CDS             complement(6248..7192)
                     /locus_tag="PKY13_08950"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT80103.1"
                     /translation="MELEPAVRRIVNVVNKLDRRDETLPWSRRRQEGAAVSRRLRGLV
                     MPPGPKMASETNVPIAVDGGHINVRVYRPHHGRLPVHLFLHGGGWCQGALDERDPRCR
                     TIAEGADCVVVSVDYRLAPENAFPVPPEDCYRSLEWVVAEADALVVDATRIGVSGESA
                     GANLAAVLCLMTRDRGGPPIAHQWLDVPATDLTMTQPSVRSTPSGYLLDYESMVEYRD
                     AYLLSEADWTTPYASPFHADDLSGLPPAWILTCGADPLRDDGTVYAARLRDAGVAVTE
                     ERLEGHVHPSFAFTRIASAAAYERRAVAALRSALHPVG"
     gene            complement(7205..>8417)
                     /locus_tag="PKY13_08955"
     CDS             complement(7205..>8417)
                     /locus_tag="PKY13_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOT80104.1"
                     /translation="TVFHLENFIAEHVTERTTSLFADDLATHHDELAARIDGRAALVI
                     GGAGTIGSSFIRALLQFRPSKLYVVDNNENGLTELTRDLRSSVDVNVPDDYKTYPINF
                     GDPVFAKLLANEGPFEIVANFAAHKHVRSEKDPYSIEAMIENNVLRAKHLLELLAGHP
                     PERFFCVSTDKAANPVNIMGATKKLMEEVILAYSAEIPITTARFANVAFSNGSLPEGF
                     LARMQRRQPLSAPEGIRRYFVSPAESGQLCLLACVLGESGDIFFPKLGEESMKTFTEI
                     GDALLRDMGYEPVPCESDATARRMAAELPDDSVVYPVHYEPADTSGEKSYEEFFTDDE
                     TLDLDRFAKLGVIKNAPRRSVDEIDEMFAVLRGLFTGEPIDKAAIVEALGRFIPNFEH
                     IETGRHLDQKM"
BASE COUNT         1374 a         2893 c         2772 g         1278 t
ORIGIN      
        1 gaaggcgcga tcgagtggat cgcaggtcaa ccgttcggca agttcctcct cgttctcatg
       61 accggcgccc tgttctcgct cgccgcctgg cggatcctcg acgccgtgtt cggcgacccc
      121 gtcgagggtg acagtgcgtc ggatcgggcc cgcttcgcgg cgaaggccgc cgtctacctc
      181 gggttcgcga tcgcgtccct ctcggccatg ctcgccaact ggtccgggac ctccggctcg
      241 ggctcaggat cgagtgacgg ccaaacgcag aaggaggcga cggcattcgt catcggctgg
      301 ccgatgggcc aatggatcgt cggcgccgtc ggcctcggcg tcatcggcta cgccgtctac
      361 atgttcaaac accatgcgat cgacgagagg ttcatgaagc gactgtcagc cgatctcacg
      421 agcgtgcgca cgctcggccg ggccggctac ggggcacgat cggtcgtctg ggcggtcgtc
      481 ggtgggctgc tcatccaagc ggcggtcacc tacgacccga acaaggccgg cggtctgtcg
      541 gccgccctcc aacggctggc acaaacgacc ggcgggcccg tgctcctcgc gctcgtcgca
      601 ctcggactgt tcgcattcgc cgccttctgt gtcgccgagg cgaagtaccg ccgagccgca
      661 tgacccacta cgaggtcgcc gtgctcccga acggggaaca cgccgcgaac tcgcgaagtg
      721 gcgcggcgcc gtcgtcgacg cctcggtccg cagcatcgct ggcttccaga acctccggga
      781 cgcggccact acccgctggc agcgtttcgc gccgcgtact agaccagtcc cgcccctgtg
      841 gcgcagttgg ttagcgcagc ggacttttaa tccgagggtc gtgggttcga gtcccaccgg
      901 gggcacgatt ggatccagtg gcctcgacgt cagtcgacgc tctggaacct tccgggaacg
      961 gactcgatcg ggaagtcgaa gtaggtgtcc ggaaacggct cgtcgcgaag ggtgtagtgc
     1021 caccactcga actcgtaggc gtcgaagccg ctcacctcca tgaccgttcg gagcgcgagc
     1081 cggttcgacg tctcgacact ggtgatcccg gtagcgccgt gatgggagac gggatccatc
     1141 aggtcgtgat gtccacccat cgcagccaac tcacccgagt cgaggtggaa cagcgtgagg
     1201 tcgaccgtgc tgccccggct gtgtccggat cgtgcggcga tgtaccccag gtcgaacatc
     1261 tccgtgcgtt cgatgttcgg gtagtgccgg tccttggcac gaccgtcctc gggttgctcg
     1321 gaccaccgcg caaacgcgtc gaccgcccgc tgaggtcggt agccgtccca gacgagcaag
     1381 ccgaacccga gggaccccgc aacgtcacgg gccagcagca aggccgcgca cagcgcttgc
     1441 gttccgacga cccggttggc ggcgtacccg tcgaccggcc ggcccgtgaa gttgtcccac
     1501 gtcgcgtact tggcgtcgca acggaccccg gggacggctt cgtcgacgaa caaccacggc
     1561 ccgctcacga cgacccgtcc gccatcgcca gggcgacgag gcgctcgatg acgtcgccga
     1621 gcgaccagcc ggcggctgcc atcatccggg gataccggct gtaggaggtc aaccccggga
     1681 gcgtgttcac ctcgttcagc accacatcgc cgtccggggt cacgaacatg tcgacccgag
     1741 cgaggccgcg gcaacccagg gcacggtaga tcgcgagtgc ggtctcgcgg acgagctgcg
     1801 ccgtctcctc ggggatgtcg gccgggacgg tgaccgtcga gttctccgag ccgtgttcgg
     1861 gatcgtcttc ctggtggatg cggaagaggc cgtgcgacaa cgagatccgg tcgaggcagc
     1921 cgacggacaa ctcggtgccg cgaccgagca cggcgcagcc cacctcggtg ccgacgacgg
     1981 cctgctcgat cacgaccttc gagtcgtacg tgcgagcagc ggccagcgcc tccgacagct
     2041 cacctccgtg ttccacacga gtcaccccga acgacgagcc cgagcgcgcc ggcttcacgt
     2101 agagcgggta ctcgaggccg gacaggtcac cgacgccgac gtcctctctg gtcacgaccc
     2161 gaaacgtcgg ggtcgagatc ccggcggcgc tgcaaatcac gtaggcgagg gacttgtcca
     2221 tgcacaacgc tgagctctgg atgtcgcagc cgacgtacgg gatgccggcg agttcgagaa
     2281 gtccctggat cgcgccgtcc tcgccggagg tgccgtgcag gacggggaac accacgtcca
     2341 accgcagcga ctcgtatcga tcgggatgct gccccggcac gaggagacca tgaaccgtcc
     2401 gatccggcga cagcaccacg ggccgacccc ggagctccca gccgtcctcc ggcccgtcac
     2461 accagctcca cgcaccatcg agggtgatgc cgaggaagac cggttcgaag cgcgcccggt
     2521 cgagatgccg agcgacctcc cgagccgact tcaccgagac ggcgtgttcc tcagagcacc
     2581 ccccgaacac gatgccgatc ctcgttcgat ccataccttc tcccttcgaa tcgagcgcag
     2641 ttgcgcaacg acgtgacgaa catctcggcg agtgcacggt cggtgtagaa cgcgacgtgc
     2701 ggggtgatga ccacgttcgg caaccgctgc aaccgggcaa tcgccgactg gtcgtcgcgt
     2761 acaccggagc agtcggcgta gaacgtcccg cgctccccct cgatgacgtc gagggcagcg
     2821 cctgcgagat gcccggtttc cagcgcggcg atgagcgcct cgttctccac gagcgcgccg
     2881 cgcccggtgt tcacgaggaa ggcgcccggc ttcatgaggg cgagccgtga tcgatcgaga
     2941 agatgatgcg tgtcgatcgt gagcggtgtg tgcagcgtga ccacgtcgct ctcgcgaagc
     3001 agctcgtcga gcgggacgtg aggaaccgag ccgctcgcgc tccggtcatg gccgagaagc
     3061 cggcacccga accctcgaag ccgatcgatg accgcggaac cgattcggcc ggttccgacg
     3121 atgccgatcg tcaggtcgcg cagctcgcgt cccggcgtct cgggcaaccg gtagtcaccg
     3181 gcatccgcgc gaccgacggt cgttcgcgcg tggcgcacgg ccatcaggat cagcatgagc
     3241 gtgtggtccg cgacgctgtc ggctgagtac tcgacgttct ccacgacgat gccgtggtgt
     3301 cgggcatcga ggacatcgat gtgatcggtg ccggcactcc tcgtgctgac gtaggtgacg
     3361 ccggatgccc ggagcgccgc gagaagctcg ggcccgacca cgcagtcgtg accgatgctg
     3421 atgcaccggt tgccggcgac cagttcgagg tggcggatgg agggagcggc cgcggtgacg
     3481 gtcgccgaca accccagccg gagcgcctcc cgttcgacca ccccgagcac gtcggggcga
     3541 cagccgaaca cggtgacccc gctcaccgca tcctcggatc gtgcgacgcg tcggggtacc
     3601 ggggcggaca gccatcggac actgccgccg accgcagctc gaaatgccac ggctcgttgt
     3661 catagatgcg gcacagtccg tacgcgacgc cgtgctcgga caaccaggcc gatgcaccgt
     3721 gtcccccgac gtcgaccgcg tcgcccgaca cgtgtgccga tcgggagggc ggcgccaccc
     3781 accgggcagc gatctcggcc gagccgtacc tcgtcacggc atctgcgagc aggtccgcct
     3841 gctcggcgga cgaccgccaa ccactcttga tgtcgagcac aacgccgtct cgatcggcgt
     3901 cggtcgtggc ccgccggagc gcgtcgagaa gatcgggcgc gagacgcgac acggccggga
     3961 cgtcgaggtc gaacaccgtg gtgccatcgg ggacgacgcg ttcggagctc gtgccggacc
     4021 actgccacgc gaccaggcac accacgacac cgacgaggac aactgcgatg gtgggacggg
     4081 tcgccgagcg cgctaccgag cgagtggagg acatggcctc actcgagcgg atccgacgtt
     4141 gtcacagcgt ctgcactttt cgatatgccg acgacaggtc gtcgctgcga acatcggggt
     4201 atgcgggtct tgatcgtcga ggatgagccg ttgctggctg acgccatccg cgacggcctc
     4261 cgcctggagg cgatcgcggc cgacgtggcc ggcgacggtg acacggcact ggcactcctc
     4321 gatgtcaaca cctacgacat cgcggtgctc gaccgcgaca tcccgggccc atcgggtgac
     4381 gaggtcgccg cccgaatcgt cgcgtccggc agtggcatgc cgatcctgat gctgactgcg
     4441 gcgagcgggc tcgacgacaa ggtgtccggg ttcgaactcg gcgccgacga ctacctgacg
     4501 aagcccttcg agctccgtga gctcgtgctc cgcttgcgag cgctcgaccg ccgccgggcc
     4561 gacccccgcc caccgatccg cgaactcgca ggcctccggc tggatccgtt ccgccgggag
     4621 gtctaccgcg acggccgcta cgtcgcactg acgaggaagc agttcgcagt gctcgaagta
     4681 cttgtcgacg cgagcggagg ggtcatcagc gccgaggaac tcctcgagcg ggcgtgggac
     4741 gcgcacgccg atccgttcac caacgccgtc cggatcaccg tctccgcgct ccgcaagcgg
     4801 ctcggcgagc cgtggctcat cgtcaccgtt cccggcgtcg gctaccgcat cgacgcgact
     4861 cccgtcggcg aggtgcccga gacgggcggc actgacggat ccggtgccga cgacggcggc
     4921 gaacgtgcgt gagcccacct ccgcacgccg tccgggaatg agcgttcgcc tcaagctcac
     4981 cctcagctat gccgggttcc tcatggtcgc cggagtgctc ctgctcgcga cggtctgggt
     5041 gttcctgctg cgctatgtgc cggcgaggat cgacgccgtc tcgagcgagt cctcccccgg
     5101 aagtccgagc acgttcgtgc ccagccgggt cgacctcctc aacgcgttcg ctccgaaagc
     5161 agcctgggcg ctcgcgttcc tcctcgtgtt cggcctcgtc ggcggatggc tcctcgccgg
     5221 cagcatgctc gcgcctctgc gccgaatcac cggtgtggca cggcaagccg gagcgggttc
     5281 gctctcacaa cgagtccaga tggagggccg caacgacgag ttccgggaac tcgccgacac
     5341 cttcgacacc atgctggccc gactcgaggc acacgtcgcc gaacaacagc ggttcgctgc
     5401 caacgcgtcg cacgagctcc gcaccccgct cgcgatcacc caggcgctct tgcaggtcgc
     5461 cagagaggac ccgacccacg acgccgacga actcgtcaaa cgcctcatcc acgtcaactc
     5521 ccgtgccatc gatctgaccg aggcgctgct gctcttcagc cgcgccgaac agggcgtgat
     5581 gagccgcgaa cccgtcgacc tctccctcct ggcagaggac gccgtcgaga cgctcctccc
     5641 actcgccgag gaccggggga tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nncctggcag aggacgccgt cgagacgctc ctcccactcg ccgaggaccg ggggatcgcg
     5821 atcgagaact ccggcgaggt ggccaccaca tcgggctcgc gcaccctgct gcagcaggtc
     5881 acgaccaacc tcgtgcacaa cgcgatcgtc cacaatgtcc cggcatccga ccgcgacgag
     5941 gcaggctcgg tgtggatcac cacgagtcct cgcgccgact gggcggccct caccgtcgag
     6001 aacaccggag acgtcctcac acccgaacac gtcgccacgc tggtcgagcc gttcaaccgc
     6061 ggggccgccc gagttcgcac cgagcacacc ggggtcgggc tcggcctcgc catcgtcgac
     6121 acgatcgtcg acgctcacgg tgggcgactc gagctcacgc cccgcagagg cggtggactc
     6181 agggcaacgg tcctgctccc actcctcaca ccgtcgagcg gaaccgccgc ggtctgagcg
     6241 ccgctcgtca gcccaccggg tgcaacgccg aacgcagggc cgccacggca cgccgctcgt
     6301 acgctgcggc cgacgcgatg cgggtgaacg cgaacgacgg gtgcacgtga ccctcgagcc
     6361 gctcctcggt cacggcgacc ccggcatcgc ggagccgcgc cgcgtaaacc gttccgtcgt
     6421 cgcggagcgg gtcggcacca cacgtcagga tccacgccgg cggcagcccc gacaggtcgt
     6481 cggcgtggaa cggcgacgcg tacggcgtcg tccagtcggc ttcgctcaac aggtaggcgt
     6541 cgcggtactc gaccatgctc tcgtagtcga ggaggtagcc cgacggcgtc gaccgcaccg
     6601 acggctgggt catcgtgagg tcggtggcag gcacatcgag ccattggtgg gcgatgggtg
     6661 ggccgccacg atcgcgggtc atcagacaca acaccgcagc gaggttggcg ccggccgact
     6721 cgccgctgac cccgatgcgg gtggcgtcga ccaccaacgc gtccgcctcc gccacgaccc
     6781 actccagcga ccggtaacag tcctcgggcg gcacggggaa cgcgttctcg ggagcgagcc
     6841 ggtagtcgac cgagacgacg acgcagtcgg cgccctcggc gatggtgcga caccggggat
     6901 cgcgttcgtc gagcgcaccc tggcaccagc cgccgccgtg gaggaacagg tgcacgggca
     6961 gtcggccgtg gtgcggccgg tagacccgca cgttgatgtg acccccgtcg acggcgatcg
     7021 ggacgttcgt ctcactcgcc atcttcggcc cgggcggcat caccaaccca cggagcctgc
     7081 ggctgacggc cgctccctcc tgacgacgac gtgaccacgg cagggtctcg tcgcggcgat
     7141 ccagcttgtt gacgacgttg acgatgcggc gaaccgccgg ctccagctcc atggccggag
     7201 actgtcacat cttctggtcg agatgacgtc cggtctcgat gtgttcgaag ttcgggatga
     7261 agcgcccgag cgcttcgacg atcgcggcct tgtcgatcgg ctcgccggtg aacagtccgc
     7321 ggaggaccgc gaacatctcg tcgatctcgt cgacgctgcg gcggggcgca ttcttgatca
     7381 cgccgagctt ggcgaagcgg tccaagtcga gggtctcgtc gtcggtgaag aactcctcgt
     7441 acgacttctc gccgctggtg tccgccggct cgtagtgaac ggggtacacg accgagtcgt
     7501 cggggagctc ggcggccatg cggcgcgccg tcgcgtcgga ctcacacggc accggctcgt
     7561 agcccatatc gcggagcagc gcgtccccga tctcggtgaa cgtcttcatc gactcctcgc
     7621 ccagcttcgg gaagaagatg tcgcccgact cgccgagcac acaggcgagc agacacagct
     7681 gacccgactc cgcgggcgac acgaagtacc ggcggatccc ttcgggcgcc gacagcggct
     7741 gccgccgttg catccgtgcc aggaagcctt cggggaggct gccgttggag aaggcgacgt
     7801 tggcgaagcg ggcggtcgtg atcgggatct ccgcggagta cgccaggatc acctcctcca
     7861 tcagcttctt ggtcgcaccc atgatgttga cggggttggc ggccttgtcg gtcgacacgc
     7921 agaagaagcg ctccggcggg tgaccggcca acagctcgag caggtgcttg gcccgtagga
     7981 cgttgttctc gatcatcgcc tcgatcgagt acgggtcctt ctcgctccgc acgtgcttgt
     8041 gcgccgcgaa gttggcgacg atctcgaacg ggccctcgtt cgccagcagc ttggcgaaca
     8101 ccgggtcacc gaagttgatg gggtacgtct tgtagtcgtc gggcacgttg acgtcgacgg
     8161 agctgcgcag gtcacgcgtg agctcggtca gcccgttctc gttgttgtcg acgacgtaga
     8221 gcttcgacgg acggaactgg agcagcgccc ggatgaagga cgaaccgatg gtgccggcac
     8281 caccgatgac cagcgccgcc cgaccgtcga ttcgggcagc gagctcgtcg tgatgggtcg
     8341 ccaggtcgtc ggcgaacaga ctggtcgtcc gttccgtgac gtgctcggcg atgaagttct
     8401 cgagatggaa cacggtg
//
LOCUS       DAOSOA010000120         8358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8461_length_8358_cov_5.293267, whole genome shotgun sequence.
ACCESSION   DAOSOA010000120 DAOSOA010000000
VERSION     DAOSOA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8461_length_8358_cov_5.293267"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PKY13_08960"
     CDS             <1..912
                     /locus_tag="PKY13_08960"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOT80105.1"
                     /translation="NADELTRRTVLEMGARGVGHIAQLCTIGRPDVGIVTAVAAAHTE
                     MFGSIDAVAAAKGELVEALPTDGTAVLNADDPRVLAMASRTSARVLTYGTSGTSADVG
                     ASDVVLDDELRAAFLLTTPWGSAPVRLGVRGRHNVGNALAAAAAALVTGVGIDDVVAG
                     LGTAQLSPSRMDLIVLPDGGIVLDDAYNANPASMRAALAALGSVDAGRRVAVVGLMAE
                     LGDDAPRLHAEIAAEARVAGIEVVAVGTDLYGGDADGVTTVADLDELGERLDPPGPGE
                     AVLLKASRVVGLDQVARRWRHGDRPAR"
     gene            complement(839..2092)
                     /locus_tag="PKY13_08965"
     CDS             complement(839..2092)
                     /locus_tag="PKY13_08965"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT80106.1"
                     /translation="MTTTTQPRVGTPRSAFAHRAFRWVFLGAFASNIGTWMQNVALLA
                     FANQLGGAKYVGIVTFAQLGPMLVLSPFGGVLADAVNRKLVMIVAATIQLMMSIGLAV
                     VASGDHPSKPLIVGLVLTMGIAAAANAPANQATLPALVDRADLAGAVSLNSAQMNASR
                     VLGPLLAVIPFLESTSTVFYFNAATYLFVIAAVAFVDFDGSPPGRSRRKPLQEIAGGI
                     IEARRDPVIGRALATVAIYSLFSLVFIYQMNGFATSELGITDSTHFRLLFGCFGLGAA
                     IGAIAVGTRLRAVDRTTLVRVGLGGFAVALGVFAMLTSAGPAYPVVGLTGFFYFIVIT
                     ALSTAIQHEVSDAARGRVMGLWMMAWAGLVPVGSLFAGYVIDDLGYRTVLLVGAGVAA
                     ALIGLADLRGASAEPPDPAPQLDSL"
     gene            2161..3297
                     /gene="wecB"
                     /locus_tag="PKY13_08970"
     CDS             2161..3297
                     /gene="wecB"
                     /locus_tag="PKY13_08970"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOT80107.1"
                     /translation="MAVQRVLSVFGTRPEAIKMAPVVAALEASPDFESRVCVTAQHRE
                     MLDQVLALFELEAHHDLDVMSAGQSPTQVLARVVAQLEPILASEQPDWVLVQGDTTTA
                     MAAGLAAAYSGAKVGHVEAGLRTFDRRQPFPEEINRLVVGVAADLHFAPTTRSRDNLL
                     REAKPPSTVFVTGNTVIDALLEVAELPAPAGPGPLDAIDPERDVILVTAHRRENFGEP
                     FRQAFEGLAELAESFADSAHLVYPVHPNPNVADVARARLGGLANVTLCDPLDYHRLVQ
                     VMARARLVITDSGGIQEEAPSLGKPVLVLRDVTERPEAVDAGTVELVGCDRDLIVKRA
                     TQLLTDSAAYDAMAQRTNPYGDGGAAARIVDALAGRPVSEFSPS"
     gene            3345..4685
                     /locus_tag="PKY13_08975"
     CDS             3345..4685
                     /locus_tag="PKY13_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOT80108.1"
                     /translation="MSGGPYGLEDTVGSAGAGMGLLLIIITPFIWSLPTALMVAEMGT
                     TIPVQGGYYHWSKTTLGPFWGFLSGWWMWVVSWVDMAIYPVLFTTFAANFGVFDFLNP
                     DTGNPWLAWLVRFAMIVLLGVLNVFGAKAIGDSSKVFLAIVLAPFVLVTIIGVFNMDR
                     NPVEPFVLEGLSPAAAFGAGLFVVMWNYMGWDSMSTFASEIDNPKKSFPKALAISIPL
                     ITLCYLLPTLVSLAIVGPDQIEWTAGAYTVIAEEVGGKWLGFIMNAAALLSAVGLYAS
                     WMLSNSRIPFALAEDGYLPASIAKLHPKYGSPWISILVCSIICSAFILGPFQGLVAID
                     VTVYAFALALEFASLIALRRSHPDLERPFKVPGGWIGVFLATLFPMLVTVAAIWFQVL
                     DVGAVQGIGWAAAAAGSGVIAYFPLRRYKARNGRDVTYTIEMIERGEFVKETAE"
     gene            4682..5197
                     /locus_tag="PKY13_08980"
     CDS             4682..5197
                     /locus_tag="PKY13_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT80109.1"
                     /translation="MSEMLRPDDWEDPVVIPDLPAMARILVPFDGSHTAERALAWAEL
                     LAGASGAEIVVMVAYQPPLTKKGRGATYVEAMRDEMQAESESLANEAVELVRSRGGAS
                     RGIVVRGEAAAAALDVAETESVDLIVIGRRGLSSELTGIGGSMERLRSSLGGGVTERV
                     ARYADAPVMVV"
     gene            5197..6282
                     /locus_tag="PKY13_08985"
     CDS             5197..6282
                     /locus_tag="PKY13_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT80110.1"
                     /translation="MSADLHLTRRSFLGATGLVGLGAVGLGGCGDNEAQRLVFYNWQD
                     YIDTKLLKDFGADAGIETVYETYASNDELSARLGQAGATRRRGRKGTTFDLIVPSDNL
                     LTDLRDADALLELGSLAGLDHLASEFRRTSFDPGNRFSVPWATGTTGIGYSMAAFPDG
                     PPDWSAFLDPAVSGKATVLDETRDAMAMALFLLGEDPNTTDASTIAAAGKKLKEVVAN
                     GVELDASTYLKRLRSGSTVLAEAFSSDVLQAMSDNDDLAFVVPDNGGLRWIDSLCIPA
                     EAPHPDIAKRFIEFYLRPEVSAANSVAVRVDTGNEAAREFVPQTVLDDPAIFPPESIV
                     DRLSFTEALDAEVEDRYSKAFAAAKGS"
     gene            complement(6301..6374)
                     /locus_tag="PKY13_08990"
     tRNA            complement(6301..6374)
                     /locus_tag="PKY13_08990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6338..6340),aa:Val,seq:cac)
     gene            complement(6391..7038)
                     /locus_tag="PKY13_08995"
     CDS             complement(6391..7038)
                     /locus_tag="PKY13_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT80111.1"
                     /translation="MGTEPSRSHYTILGVGPRATSKEIRVAYLNLARALHPDRVRSGS
                     PAEQQLAGRRMREVNEAYEVLKDTARRQAYDRDHGVSTARPTSGSGSGTGGASRAPGG
                     PSAATDPSRPRARPPGNPERFDADPAWRDQYPGGHSAWMDDVDDVEVSPFVAFVLQRG
                     PLIAVLVLAAFLFIATAYAGGGASDEPGPTAPTSIECPDPSTTSPSGGSVDVQPC"
     gene            complement(7045..8208)
                     /locus_tag="PKY13_09000"
     CDS             complement(7045..8208)
                     /locus_tag="PKY13_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80112.1"
                     /translation="MSTPAGSRAIFTDEHEMFRSAYRSFLDREMVPRAHEWEQAGIVS
                     RDVWTSAGASGFLAFDIPETYGGPGVEDFRYNLVVVEELASAGILAAGAGFTLHNDVT
                     LPYFMKYCTEEQKQRWLPGMASGDLITAVAMTEPGMGSDLASMKTTAIKDGDTYVLNG
                     SKTFITNGINSDLVIVAAKTDPNERHKGMSLLVVERGMDGFERGRNLDKIGLHAQDTA
                     ELFFNDVRVPAENLLGSEGQGFFQLVNNLPQERLGIAAAGVAHARAALGWTLAYCKER
                     TAFGQPIGSFQNSRFKLAEMATEVEIGQTFVDRCVLELMEGTLTAETAAMAKWWCTEM
                     QKRTVDTCLQLHGGYGYMLEYPVATAYLDARITTIYGGTTEIMKEVIGRSLGV"
BASE COUNT         1245 a         2770 c         2971 g         1372 t
ORIGIN      
        1 aacgccgacg agctgacgcg gcggacggtc ctcgagatgg gtgcccgcgg ggtcggccac
       61 atcgcccagc tctgcacgat cggtcgcccc gacgtcggga tcgtcacggc cgtcgctgcg
      121 gcccacaccg agatgttcgg gtcgatcgac gccgtcgcgg ccgcgaaggg tgagctggtc
      181 gaggcgctcc cgaccgacgg cactgcggtg ctgaacgccg acgacccgcg ggtgctggcg
      241 atggcgtccc ggacgtcggc ccgggtgctg acgtacggca cgtccggcac gtccgccgac
      301 gtcggcgcat ccgatgtggt gctcgacgac gagctgcggg cggcgttcct gctgaccacg
      361 ccgtggggtt cggcgccggt tcgactcggg gttcggggtc gccacaacgt gggcaacgcc
      421 ttggcggcgg ccgcagcggc gctggtgacg ggcgtcggaa tcgacgatgt cgtcgccggg
      481 ctgggcaccg cgcagctctc gccgtcgcgg atggatctga tcgtgttgcc cgatggcggc
      541 atcgtgctcg acgacgccta caacgccaac ccggcgtcga tgcgggcggc gctcgccgcg
      601 ctcggatcgg tcgacgccgg tcgccgtgtc gccgtggtcg gcctgatggc cgagctcggc
      661 gacgacgctc ctcggttgca cgccgagatc gccgccgagg ccagggttgc cggcatcgag
      721 gtcgttgccg tcggcaccga tctctacggc ggcgacgccg acggcgtgac gacggtggcg
      781 gacctcgacg agctggggga gcggctggac ccgccggggc ccggcgaggc ggtactgctc
      841 aaagcgagtc gagttgtggg gctggatcag gtggctcggc gctggcgcca cggagatcgg
      901 ccagcccgat gagggcggcc gccacgccgg cgccgaccag gagcacggtg cggtacccga
      961 ggtcgtcgat cacgtaaccg gcgaacaggc tgccgacggg gaccaggccg gcccacgcca
     1021 tcatccacag gcccatcacc cgcccgcgcg cggcgtcgct cacctcgtgc tggatcgccg
     1081 tggacagggc ggtgatcacg atgaagtaga agaacccggt gaggccgacg acgggatacg
     1141 cgggccccgc cgaggtcaac atcgcgaaca ccccgagcgc gacggcgaat ccgcccaacc
     1201 cgacccggac gagggtcgtg cggtcgaccg cccggagacg ggtaccgacg gcgatcgctc
     1261 cgatcgcggc gcccagcccg aagcacccga agagcagccg gaagtgcgtg ctgtcggtga
     1321 tgcccagctc cgacgtggcg aacccgttca tctggtagat gaacacgagc gagaagagcg
     1381 agtagatcgc cacggtggcg agcgcccggc cgatcacggg gtcgcggcgg gcctcgatga
     1441 tgcccccggc gatctcctgg aggggtttgc ggcgggagcg tcccggcggg gacccgtcga
     1501 agtcgacgaa cgcgacggca gcgatgacga acaggtacgt ggcggcgttg aagtagaaga
     1561 ccgtgctcgt cgactcgagg aacgggatca cggcgagcag tggtccgagc acacgcgatg
     1621 cgttcatctg cgccgagttg agcgagacgg cgccagcgag gtcggcgcgg tcgacgagcg
     1681 ccggcagcgt cgcctggttg gccggtgcgt tggcggcggc agcaatgccc atcgtgagca
     1741 ccaggccgac gatcaggggt ttggacggat ggtcgcccga cgcgacgacg gcgaggccga
     1801 tgctcatcat gagctggatc gtggcggcaa cgatcatcac gagtttgcgg ttcaccgcgt
     1861 cggccagcac gccgccgaac ggcgagagga cgagcatcgg accgagttgg gcgaacgtga
     1921 cgatgcccac gtacttggcg ccgccgagct ggttggcgaa cgccagcagc gcgacgttct
     1981 gcatccaggt gccgatgttc gacgcgaacg ccccgaggaa cacccagcgg aaggcccggt
     2041 gggcgaacgc cgaccggggc gtccccacgc ggggctgggt cgtcgttgtc actgctccag
     2101 actacggcgt gggcgcgacg gcccattccc ggatttccgg tgtcgtcgta tcctggcgcc
     2161 atggcggttc agcgagttct ctccgtgttc ggcacgcgcc ccgaggcgat caagatggcc
     2221 ccggtggtcg cggcgctcga ggcatccccc gacttcgagt cccgggtgtg tgtgacggcg
     2281 cagcatcgcg agatgctcga ccaggtgttg gcattgttcg agctcgaggc gcatcacgac
     2341 ctcgacgtga tgagcgccgg ccagtcgccc acccaggtgc tggcgcgtgt ggttgcgcag
     2401 ctcgaaccga tcctggcctc cgaacagccc gactgggtgc tcgtgcaggg cgacacgacc
     2461 acggcgatgg cggcggggct cgcggctgcg tactccggcg cgaaggtcgg ccacgtcgag
     2521 gccggcctgc ggacgttcga ccgtcgccag ccgttccccg aggagatcaa ccggctcgtg
     2581 gtgggggtgg ccgccgacct gcacttcgcc ccgaccaccc ggagccgtga caacctcctt
     2641 cgggaggcga agccgccgtc gacggtcttc gtgaccggca acacggtgat cgacgccctg
     2701 ctcgaggtgg ccgaactacc ggcccctgca ggccccggtc cgctcgacgc catcgatccc
     2761 gagcgcgacg tgatcctggt gacggcgcac cgacgtgaga acttcgggga gcccttccgg
     2821 caggcgttcg agggattggc cgagctggcc gagtccttcg ccgattctgc ccatctcgtc
     2881 tacccggtgc acccgaaccc gaatgtggcc gacgttgccc gggcccgact cggtggtctc
     2941 gccaacgtca cgttgtgcga cccgctcgac taccaccggc tcgtgcaggt catggcgcgg
     3001 gcccggctgg tgatcaccga ttccggtggc attcaggagg aggcgccgtc gctcggcaag
     3061 ccggtgctgg tgctccgcga cgtgaccgag cggcccgagg ccgtcgacgc cggaacggtc
     3121 gaactcgtcg gttgtgatcg cgacctcatc gtgaagcggg ccacgcagct gttgaccgac
     3181 tctgctgcct acgacgccat ggcccagcgg accaatccct acggcgatgg cggggcggca
     3241 gcccgcatcg tcgacgccct cgcggggcgc cccgtgtccg agttcagtcc gtcgtagccc
     3301 tcagtccctg gcggagcgga tcgttcggca ggcgctgccg ttccgtgtcg ggcggcccgt
     3361 acggcctcga ggacaccgtc ggctcggcgg gtgcgggtat gggcctgctg ctcatcatca
     3421 tcaccccgtt catctggagc cttccgacgg ccctcatggt cgccgagatg ggcaccacga
     3481 ttccggtgca gggtggctac taccactggt ccaagacgac cctgggcccg ttctgggggt
     3541 tcctgtcggg ctggtggatg tgggtcgtgt cctgggtgga catggcgatc tatcccgtgc
     3601 tgttcaccac gttcgccgcc aacttcggtg tgttcgactt cttgaatccc gacacgggca
     3661 acccgtggct ggcctggctg gtgcggttcg ccatgatcgt gctgctcggc gtgctcaacg
     3721 tgttcggcgc gaaggcgatc ggcgacagct ccaaggtgtt cctggcgatc gtgctcgcgc
     3781 cgttcgtgct cgtcacgatc atcggcgtct tcaacatgga ccggaacccg gtcgagccgt
     3841 tcgtgctcga gggattgtcg cccgccgcgg ccttcggggc cggcctcttc gtggtcatgt
     3901 ggaactacat gggttgggac tcgatgtcga cgttcgccag cgagatcgac aacccgaaga
     3961 agagcttccc gaaggcgctt gcgatctcga tcccgctgat cacgctctgc tacctgcttc
     4021 ccaccctggt ctcgttggcg atcgtcggcc ccgaccagat cgaatggaca gccggcgcct
     4081 acaccgtcat cgccgaggag gtcggtggaa agtggctggg gttcatcatg aacgccgccg
     4141 ccctgctgtc cgccgtcggc ctgtacgcct cgtggatgct ctccaacagc cgcatcccct
     4201 tcgcgctcgc ggaggacggc tacctgccgg catcgattgc caagctgcac cccaagtacg
     4261 gctcgccgtg gatctcgatt ctcgtgtgct cgatcatctg ctcggccttc atcctcggcc
     4321 cgttccaagg tctcgtcgcc atcgacgtca cggtgtacgc cttcgcactg gcgctcgagt
     4381 tcgcttccct gatcgccctg cgccgcagcc atcccgatct cgaacgcccg ttcaaagtgc
     4441 cgggtggttg gatcggggtg ttcctcgcaa cgctctttcc gatgctcgtc accgtcgccg
     4501 cgatctggtt ccaggtcctc gacgtcgggg ccgtgcaggg catcggatgg gcggctgcgg
     4561 ccgccggctc cggggtgatc gcctacttcc cgcttcgtcg atacaaggcg cgcaacgggc
     4621 gtgatgtgac ctacacgatc gagatgatcg aacgcggtga gttcgtgaag gagacagccg
     4681 aatgagcgag atgctgcgac ccgacgattg ggaagatccc gttgtcatcc ccgacctgcc
     4741 ggcgatggcg cgcatcctcg tgccgttcga cgggtcccac accgccgagc gggcgctcgc
     4801 gtgggccgag ctcctcgccg gtgcctccgg tgcggagatc gtggtgatgg tggcgtacca
     4861 acccccgctc accaagaagg ggcgtggtgc cacctacgtc gaggcgatgc gtgacgagat
     4921 gcaggccgag tccgagtcgc tcgccaacga ggccgtcgag ctggtgcggt cgcgtggcgg
     4981 cgcctcgcgc ggcatcgtcg tgcgcggcga agcggcggca gcggccctcg acgtcgcgga
     5041 gaccgagagc gtcgacctca tcgtgatcgg ccggcgcggc ctgtcgtcgg agctcacggg
     5101 catcggcggt tcgatggaac ggctccgatc gagcctgggc ggcggcgtga ccgaacgagt
     5161 ggcgcgctac gccgacgctc cggtgatggt cgtctgatga gcgccgatct gcacctcacg
     5221 cggcgatcgt tcctcggggc gacgggcctc gtgggcctcg gggccgtcgg tctcggcggt
     5281 tgtggtgaca acgaggcaca acgcctcgtc ttctacaact ggcaggacta catcgacacg
     5341 aagctgctga aggacttcgg tgccgacgcg ggcatcgaga ccgtgtacga gacctacgca
     5401 tccaacgacg agttgagcgc tcgcctcggg caggcgggcg cgacccggcg acgtgggcgc
     5461 aagggaacga cgttcgacct gatcgtcccg tcggacaacc tgctcaccga tctccgcgat
     5521 gccgatgcgc tcctggaact cggttcgctc gctggtctcg accatcttgc ctcggagttc
     5581 cggcgcacgt ccttcgaccc cggcaaccgc ttcagcgtcc cgtgggcgac cgggacgacc
     5641 ggtatcggct actcgatggc ggcgttcccc gatgggccgc ccgactggtc ggcgttcctc
     5701 gacccggcgg tgtcgggcaa ggccaccgtc ctcgacgaga cccgcgacgc catggcgatg
     5761 gcgttgttcc tgctcggcga ggacccgaac accactgacg catcgacgat cgccgcagcg
     5821 gggaagaagt tgaaggaggt cgtcgccaac ggtgtcgagc tcgacgcgtc gacctacctg
     5881 aagcggcttc gttccggttc gacggtgttg gccgaggcat tctcgtcgga cgtcctgcag
     5941 gccatgtccg acaacgacga cctggcgttc gttgtgcccg acaacggtgg gctgcggtgg
     6001 atcgacagtc tgtgcattcc ggcggaggcg ccccaccccg acatcgccaa gaggttcatc
     6061 gagttctatc tccgacccga ggtctcggcg gccaactcgg tcgcggtgcg ggtcgacacg
     6121 ggcaacgagg ccgcgcggga gttcgtgcca caaacagtcc tcgacgaccc ggcgatcttc
     6181 ccgcccgagt cgatcgtcga tcggctgtcc ttcaccgagg cgctcgacgc tgaggtcgag
     6241 gatcgatact cgaaggcgtt cgcggccgcc aagggatcct gacccggggc tgcgacgggg
     6301 tgggcgatgg cagactcgaa ctgccgacct cacgcgtgtg aagcgtgcgc tctaaccaac
     6361 tgagctaaac gcccgaaagc agcggccacc ttaacacggc tggacgtcga ccgatccacc
     6421 cgatgggctc gtcgtcgacg ggtcgggaca ctcgatcgag gtcggcgccg tggggcccgg
     6481 ctcgtcggac gcgccgccgc ccgcataggc ggtggcgatg aacaggaacg cggcgagcac
     6541 gagcacggcg atgaggggcc cgcgttgcag cacgaacgcg acgaacggcg acacctcgac
     6601 gtcgtcgaca tcgtccatcc acgccgagtg gccaccgggg tactggtcgc gccatgccgg
     6661 gtcggcgtcg aagcgttcgg gattgcccgg aggtcgggcc cgcggtcgtg atgggtccgt
     6721 cgccgctgac gggccgccgg gtgctcggct cgcgccgccg gtgcccgagc cgctgccgct
     6781 cgtcgggcgt gccgtcgaga cgccgtgatc gcggtcgtag gcctgacgcc gagccgtgtc
     6841 cttgagcacc tcgtacgcct cgttgacctc gcgcatgcgc cgaccggcga gctgctgctc
     6901 ggccggtgac ccggagcgga cgcggtcggg gtggagcgcc cgggccaggt tcaggtatgc
     6961 gacgcggatc tccttggagg tggcccgcgg cccgaccccg aggatcgtgt agtggctccg
     7021 gctcggctcg gtgcccatcg gggatcagac gcccaacgag cgcccgatca cctccttcat
     7081 gatctcggtc gtgccgccgt agatggtggt gatgcgggca tcgaggtagg ccgtcgcgac
     7141 ggggtactcg agcatgtacc cgtagccacc gtgcagctgc aggcacgtgt cgacggtgcg
     7201 cttctgcatc tcggtgcacc accacttggc catcgccgcg gtctctgcgg tgagggtgcc
     7261 ctccatgagc tcgagcacgc agcggtcgac gaaggtctgg ccgatctcga cctcggtggc
     7321 catctcggcg agcttgaacc ggctgttctg gaacgagccg atcggctggc cgaacgcggt
     7381 gcgttccttg cagtaggcga gcgtccagcc caatgcggcc cgcgcgtggg cgacgccggc
     7441 ggcggcgatg ccgaggcgct cctgggggag gttgttgacg agctggaaga acccctggcc
     7501 ctcggagccg agcaggttct cggcgggcac gcggacgtcg ttgaagaaca gctcggcggt
     7561 gtcctgggcg tgcagaccga tcttgtcgag gttgcggccg cgttcgaagc cgtccatgcc
     7621 gcgctcgacg acgaggaggc tcatgccctt gtggcgctcg ttggggtcgg tcttggcggc
     7681 gacgatgacg aggtcggagt tgatgccgtt ggtgatgaac gtcttggagc cgttgaggac
     7741 gtaggtgtcg ccgtccttga tggcggtcgt cttcatcgac gcgaggtcgg atcccatgcc
     7801 gggctcggtc atggcgacgg cggtgatgag atcgcccgat gccatgcccg gaagccagcg
     7861 ctgcttctgc tcctcggtgc agtacttcat gaagtacggg agcgtgacgt cgttgtggag
     7921 tgtgaacccg gcgcccgcgg cgaggatgcc ggccgaggcc agttcctcga ccaccacgag
     7981 gttgtaacgg aagtcttcga cgccgggccc gccgtaggtc tcggggatgt cgaaggcgag
     8041 gaagcccgac gcgccggcgg acgtccagac gtcgcggctg acgatgccgg cctgctccca
     8101 ttcgtgggcc cgcggcacca tctcccggtc gaggaaggag cggtaggcgc tccggaacat
     8161 ctcgtgctcg tcggtgaaga tcgctcgcga tcccgcaggt gtgctcatgg gcgtgagctt
     8221 cccacgatct cggcgggtcg gcacacccgg tcagaacccg ccgaagtcgt cgggggagcc
     8281 gccgttcctc atgatggcgg cgaagaacag cacgaacagc gccaccgtga cgacgagggc
     8341 gatcagtgcg tagacgag
//
LOCUS       DAOSOA010000121         8342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8488_length_8342_cov_7.541209, whole genome shotgun sequence.
ACCESSION   DAOSOA010000121 DAOSOA010000000
VERSION     DAOSOA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8488_length_8342_cov_7.541209"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PKY13_09005"
     CDS             complement(<1..681)
                     /locus_tag="PKY13_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT80113.1"
                     /translation="MVAPRASISRSRQRLPSRTGTLPVVIALVTLGLFAVVFVFSPTL
                     NPADLLAGRFLEVPVPKVTGLTQDRALIELERGRLHGDVHFVYSSTVQRGKVVRQKPL
                     AASSMRRDSTADIYVSRGPEFVPLPELVGQKRSDVISTIRELGLEVAEERVNDETAPA
                     GDVIAQSPAPGTVVEGGATVSISVSTGPMTRTVPEVTGLPIEGAAFNLGKAGFTPSTF
                     SSLSTISTP"
     gene            complement(684..2681)
                     /gene="pknB"
                     /locus_tag="PKY13_09010"
     CDS             complement(684..2681)
                     /gene="pknB"
                     /locus_tag="PKY13_09010"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOT80114.1"
                     /translation="MTSSDPTGSAAPVVLGGRYEVHRRLARGGMAEVFLARDQALDRP
                     VAVKILFPEYATDPAFVERFRREAQAAANLSHPNIVGVYDWGSESGTYYIVMEYVEGQ
                     SLAEVIRDAGPLHPRRAAEVIFEVAGALGFAHQRGVVHRDVKPGNVLISTSGTAKVTD
                     FGIARALSGPADDLTQAGSVMGTATYFSPEQAQGFQVDARSDLYSLGVVLYEILCGRP
                     PFVGDSPVAIAYKHVQEWPPRPSQFVTGVPAGLESVILKLLAKKPEQRYRSADDLRAD
                     LRRFLDGQTTLAEEELARPAAAPAAAATSAATVVNPAVTADPSHTTVMPAQQAPVPAE
                     PDEYDDEPPARTGLFVALTVLLLVGLGALGFWLVSSLNDDADVSDGVLVTNTVDMTKD
                     DAVAALQKQGFNPVVTEEASDDAVVGTVFRQNPAANTRLAKGKDVEIFVSTGPANVVI
                     PPVANLSEAEATKALTDLKFTVKVEPFESDTVEEGKAVETSPPAGQPAPAASEVLLRI
                     STGAGSEVVPVVTGKTVDAAKKALTDKGFVVGETIQQASADVKKDLVITTDPVGSAPK
                     KSTINLIVSTGAEPVKVPAVKGQREDEATALLKGKGFDVSVEPKALPPGDANDGRVIS
                     VDPAAGTSIDAGSTVTITVGVAGAATTTAPTSSTTTTTTSP"
     gene            complement(2780..4312)
                     /locus_tag="PKY13_09015"
     CDS             complement(2780..4312)
                     /locus_tag="PKY13_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT80115.1"
                     /translation="MNRQLRKLAIALMACYLALFVKLNFLQVIDAKALNDEPGNGRAV
                     VRDFNRPRGDIVTADGTTVAHSEESDGRYKYQRTYPTDDLFAQTVGSYSLLFGTDGVE
                     KRYNDELSGNTTDFRVRGFLDPFLEEPNVGTVEVTLRADVQQVAKEALGNKPGSVVAL
                     DPRTGAVLAMWSYPSYNPNLTSLNDATAARAFKEAYDKQADKPLLAKTFRERYFPGST
                     FKVVTASAGLESGKVTETTPSFPVSTGYQPPLTTRTIRNFDGANCGGTLFELLRVSCN
                     GGFAAMGAEVLGPEIMIGGAEAFGFNDVPPIDLPGAVRSVFPTDFGKRLRDGDDPGDA
                     AVYEKTPQLAQASIGQNDVSATPLQMAMVAGAIGNGGTMMTPHVMAEVRDRKQESIDT
                     YQPSVWKTPVSKETAEIMRRGMVEVATNGTAKAMAIGGVEVGGKTGTAQLGTPDPKSH
                     AWVIGFAGPAGQPAQVAVAVLVQGQPGASEQTGGRVAAPIAKRVLEAALAAPVQPGTA
                     GR"
     gene            complement(4326..5666)
                     /locus_tag="PKY13_09020"
     CDS             complement(4326..5666)
                     /locus_tag="PKY13_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOT80116.1"
                     /translation="MTVALAPHGRNRRATEAGLLALAGLIIGVAFALVSLGRTKALPA
                     NLIPFVVAVFVLFLGAHIGVRRLAPRADPLILPVAVMLNGLGYVMIARLNPKLASQQA
                     GWTAVGVIGFIVTLAVIKRVRTLERVRYTVGLGGVLLLVLPLIPGLGVRINGARIWVR
                     LGIVNFQPGEFAKIALAIFFAAYLVERRELLGTATFKVGPLMTPDPKHLLPVLVAWGF
                     ALMVMMFQRDLGSALLFFVLFMTVLWIATGRGSYLAVGTTLFGGGAYLSWALFSHVQD
                     RVSMWLNPWQDAQDKGYQIIQSTYAFAWGGVAGTGLGVGLGNRVPIAESDFIFAIIGE
                     ELGLLGATAVLVLFLLLVGSGLRVAMRSTDPFAKLLAAGLTTLIGVQSFIIMAGVTRL
                     LPLTGLTLPFVSYGGSSLISNWVLIALLVRLSDETAGRAEPVGDDETTVLAMAS"
     gene            complement(5663..6940)
                     /locus_tag="PKY13_09025"
     CDS             complement(5663..6940)
                     /locus_tag="PKY13_09025"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOT80117.1"
                     /translation="MRLEVGSGTDVGQLRSLNEDSLLVADGLFAVADGMGGHRGGEVA
                     SARALVAFEQSAATPTLESLMAAVQAANADVLEHARSSPELMGMGTTLCALAPLDDRR
                     VAIVNVGDSRVYLLRGQALFQVSEDHSFVETLVREGRLTRAQADVHPQRNVITRALGI
                     EPHIDVDGWEVLVADGDRFLLCSDGLFNEVGDSDIELILVELTDPVEAAGRLIDAANA
                     GGGRDNITCVVVDVHDVGAGSAEAAAIAVGRPIDQSITRRSTAADFDETSVDDPNLSA
                     SNARPTPAEAAAPAKVRRPRRLTWRTVVFLLLIVLVFAAALFATDWYYKNTYSVGVSD
                     EQVVIFRGPPDTDILWFKTEILPTDVAVDDLRDAEQRLVEQGVGDLSLAEANRYVERL
                     EAQAAAATTTTTTTTTTTTTTAPGAETTVTTIP"
     gene            complement(6940..7434)
                     /locus_tag="PKY13_09030"
     CDS             complement(6940..7434)
                     /locus_tag="PKY13_09030"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT80118.1"
                     /translation="MPDELITIFKICFLVLLYLFFFRVLRAVWTEVTAVPAGLAPEAP
                     TKAPKAPKPAKTPRKAKVKQPTEPTVPTRLVAVDPPHLVGIEYPLGEGMSIGRGGDST
                     VVIEDSFLSTRHAAFERRDGEWVVEDEGSTNGTYVNNVKVERIARLHLGDRVQVGNVI
                     LEVQ"
     gene            complement(7445..8104)
                     /locus_tag="PKY13_09035"
     CDS             complement(7445..8104)
                     /locus_tag="PKY13_09035"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HOT80119.1"
                     /translation="MGLSDFERKLEKGVEGVFGRVFRSGVRPVELGRKLVREMDVHRT
                     VGVNGATMAPNSFVLVLAEADFEQLADMEDTLANELTQLAKTHASEERYRFAGPVEVF
                     FEVEPSQRPGVVTIDARFRQPEPGTAIAALILPGGDRVPLSNEVVSVGRASDCTIVLG
                     DQNASRRHAEFRPVEGGFAIIDLASTNGTKVNGRNVTEHLLKHGDEVLFGATSIRFEQ
                     D"
     gene            8201..8284
                     /locus_tag="PKY13_09040"
     tRNA            8201..8284
                     /locus_tag="PKY13_09040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8235..8237,aa:Leu,seq:cag)
BASE COUNT         1310 a         2683 c         3011 g         1338 t
ORIGIN      
        1 cggggtgctg atcgtggaca gtgaactgaa ggtgctgggc gtgaacccgg ccttgccgag
       61 gttgaacgct gcgccttcga tgggcaggcc ggtcacctcg ggcacggttc tcgtcatggg
      121 gccggtcgac acgctgatcg acacggtggc accgccttcg acgacggtgc ccggagccgg
      181 cgactgcgcg atcacgtcac ccgccggtgc ggtctcgtcg ttgacccgtt cctcggccac
      241 ctcgaggccc agctcacgga tcgtggagat gacgtcggac cgcttctgac ccacgagttc
      301 gggcagcggc acgaactccg gtccacgact cacgtagatg tcggcggtcg agtctcgacg
      361 catgctcgac gcggcaagcg gcttctgccg gacgaccttg ccgcgctgca cggtggacga
      421 gtacacgaag tggacgtcgc cgtggagacg cccacgttcg agctcgatca gcgctcggtc
      481 ctgggtcagt ccggtgacct tcgggaccgg cacctcgagg aaccgaccgg ccagcaggtc
      541 tgcggggttg agggtgggag agaacacgaa caccacggcg aacagaccga gcgtcaccaa
      601 cgcgatgacg accgggaggg tccccgtgcg gctcgggagt cgctggcggg accgcgagat
      661 cgaggcccgg ggcgcgacca cgactacggg ctcgtggtcg tcgtggtggt cgaactggtc
      721 ggagccgtcg tcgtggcagc accggcgacg ccgacggtga tcgtgacggt ggacccggcg
      781 tcgatcgagg tgccggcagc cgggtcgacc gagatcacac ggccgtcgtt ggcgtcaccg
      841 ggcgggaggg ccttgggctc gacgctgacg tcgaatccct tgcccttgag gagcgcggtc
      901 gcctcgtcct cgcgctggcc cttgactgcg gggaccttca cgggttccgc tccggtcgag
      961 acgatgaggt tgatcgtcga cttcttcgga gcagaaccga cggggtcggt cgtgatgacg
     1021 aggtccttct tcacgtcggc cgacgcctgc tggatcgtct cgccgacgac gaatcccttg
     1081 tcggtcagcg ccttcttggc agcgtcgacg gtcttgccgg tgacgaccgg aacgacctcc
     1141 gagccggcgc cggtcgagat gcgcaacaac acctcgctgg cggcgggtgc cggttggccg
     1201 gcgggcgggg acgtctcgac ggccttgccc tcctcgacgg tgtccgattc gaacggctcg
     1261 accttcacgg tgaacttgag gtcggtcagc gccttggtcg cctcggcctc cgagaggttc
     1321 gccaccggcg ggatgaccac gttggccgga ccggtgctca cgaagatctc gacgtccttg
     1381 cccttggcga ggcgggtgtt ggccgccggg ttctgacgga acacggtgcc cacgacggca
     1441 tcgtcggacg cttcctcggt gaccaccggg ttgaagccct gtttctggag cgcggccacc
     1501 gcgtcgtcct tggtcatgtc gaccgtgttc gtgacgagga ccccgtcgct cacgtcggcg
     1561 tcgtcgttga ggctcgagac gagccagaac ccgagggcgc cgaggccaac gagcaacagc
     1621 acggtgagcg cgacgaacag gccggtgcgg gcggggggtt cgtcgtcgta ctcgtcgggt
     1681 tcggcgggga cgggtgcctg ctgggcgggc atgaccgtgg tgtggctggg atccgcggtg
     1741 accgcagggt tgaccacggt cgcggcggag gtggcggcag ctgccggggc cgccgccggt
     1801 cgggccaact cctcttcggc gagggtcgtc tgaccgtcga ggaagcggcg gaggtcggcg
     1861 cggaggtcgt cggccgagcg gtagcgctgc tcgggcttct tggccaggag cttgaggatc
     1921 acggactcga ggccggccgg cacgcccgtc acgaactgcg acggacgggg cggccattcc
     1981 tgcacgtgct tgtaggcgat cgcgaccggc gagtcgccga cgaacggcgg acggccgcac
     2041 aggatctcgt agagcaccac gccgagcgag tacaggtcgc tgcgggcgtc cacctggaag
     2101 ccctgggcct gctcgggcga gaagtatgtg gcggtgccca tgaccgaacc ggcctgggtg
     2161 aggtcgtcgg cgggacccga cagcgcccgg gcgataccga agtcggtcac cttggcggta
     2221 ccggaggtgg agatcaggac gttgcccggc ttcacgtcgc ggtgcaccac accacgctgg
     2281 tgggcgaacc cgagtgcgcc ggccacttcg aagatgacct cggcggcacg acggggatgc
     2341 agcgggccgg cgtcgcggat gacctcggcg agggactgac cctcgacgta ctccatgacg
     2401 atgtaatagg tgccgctctc cgaaccccag tcgtacacgc cgacgatgtt ggggtgggag
     2461 aggttggcgg cggcctgggc ctcgcgccgg aagcgctcga cgaatgccgg gtcggtcgcg
     2521 tactcgggga agaggatctt gacggccacc gggcggtcga gggcctggtc gcgggccaag
     2581 aacacctcgg ccatgccgcc gcgcgccaag cgtcggtgca cctcgtaccg tccgccgaga
     2641 acgactggcg cggcggaacc cgtggggtcg gaggaagtca tggcagcctc agactagtga
     2701 gcgccgcgcg ttcaaccgtg gcactccgaa agacgacaaa cgggctcaac atgttgcgac
     2761 gaggggcgtg ggtcggtggt cagcggccgg ccgtgcccgg ctggacaggg gccgccaggg
     2821 ctgcttccag cacccgcttg gcaatggggg cggccacccg accaccggtc tgctcgctgg
     2881 cgccgggttg gccctgcacg agcacggcga ccgcgacctg cgccggttgg ccggcgggcc
     2941 cggcgaagcc gatgacccat gcgtgcgact tggggtcggg ggtgccgagc tgtgccgttc
     3001 cggtcttgcc gccgacctcg acgccgccga tcgccatggc cttggcggtg ccgttggtcg
     3061 cgacttcgac catgccgcgc cgcatgatct ccgcggtctc cttggagacg ggggtcttcc
     3121 ataccgacgg ctggtaggtg tcgatcgact cctgcttgcg atcccgcacc tcggccatca
     3181 cgtggggggt catcatcgtc ccgccgttgc cgatcgctcc ggccaccatc gccatctgca
     3241 gcggcgttgc cgacacgtcg ttctgaccga tggatgcctg ggcgagttgt ggggtcttct
     3301 cgtacaccgc ggcgtcaccg gggtcgtcgc cgtcgcggag gcgtttgccg aagtcggtcg
     3361 ggaagacgga ccgcacggcg ccgggcagat cgatcggcgg cacgtcgttg aacccgaagg
     3421 cctcggctcc accgatcatg atctccggac cgagcacctc ggcgcccatt gcggcgaagc
     3481 cgccgttgca cgagacgcgt aggagttcga agagcgtgcc gccgcagttc gcgccgtcga
     3541 agttacggat cgtccgggtc gtgagcggtg gctggtatcc ggtggacacc gggaacgacg
     3601 gcgtggtctc ggtgaccttg cccgattcga ggccggcgga cgcggtgacg accttgaacg
     3661 tcgaaccggg gaagtagcgc tctcggaacg tcttcgcgag cagcggcttg tcggcctgct
     3721 tgtcgtaggc ctccttgaag gcacgcgccg cggtcgcgtc gttcagcgag gtgaggttcg
     3781 ggttgtacga cgggtacgac cacatggcga ggacggcgcc cgtgcggggg tcgagggcga
     3841 cgaccgagcc cggcttgttg ccgagggcct ccttggcgac ctgctggacg tcggcgcgga
     3901 gtgtgacctc gacggtgccg acgttgggtt cctcgaggaa cgggtcgaga aagccccgaa
     3961 cccggaagtc ggtcgtgttg cccgacagtt cgtcgttgta gcgcttctcg accccatcgg
     4021 tcccgaagag gagggagtac gagccgaccg tctgggcgaa caggtcgtcg gtgggatacg
     4081 tgcgctgata cttgtaccgg ccgtcgctct cctcggagtg agcgacggtc gtgccgtcgg
     4141 ccgtgacgat gtcgccgcgg ggtcggttga agtcgcgtac gacggcgcgt ccgttgcccg
     4201 gctcgtcgtt gagggccttg gcgtcgatga cctgcaggaa gttgagcttg acgaacagtg
     4261 cgaggtagca cgccatcaag gcgatcgcga gcttgcggag ttgccggttc atgccgtcac
     4321 cgcgctcacg acgccatcgc caggacggtc gtctcgtcgt cgcccacggg ctcggcccgg
     4381 ccggcggtct cgtcggacag gcggacgagc aacgcgatca gcacccagtt ggagatgagc
     4441 gacgagccgc cgtaggagac gaacggcaag gtgagacccg tcagcgggag gagtcgagtg
     4501 acgccggcca tgatgatgaa cgactggacg ccgatcagcg tggtcaggcc ggcggcgagg
     4561 agcttggcga acgggtcggt cgagcgcatc gccactcgca ggccgctgcc gacgagcagc
     4621 aggaacagta cgagcaccgc ggtcgcgccg agcaacccga gctcttcgcc gatgatcgcg
     4681 aagatgaagt cgctctcggc gatggggact cggttgccca ggccgacacc gagcccggtg
     4741 cccgccaccc cgccccaggc gaacgcgtac gtcgactgga tgatctggta gcccttgtcc
     4801 tgggcgtcct gccacgggtt gagccacatc gagacgcggt cctgcacgtg actgaagagc
     4861 gcccagctga ggtaggcgcc gcctccgaac agcgtggtgc cgaccgccag gtacgaccca
     4921 cgcccggtgg cgatccacag cacggtcatg aacagcacga agaacagcag tgccgagccg
     4981 aggtcgcgct ggaacatcat gaccatgagc gcgaagcccc acgcgacgag caccgggagg
     5041 aggtgcttgg ggtcgggtgt catgagcggc ccgaccttga aggtcgccgt accgaggagt
     5101 tcgcggcgtt cgaccaggta ggcggcgaag aagatggcga gggcgatctt ggcgaactca
     5161 ccgggctgga agttgacgat gccgagccgg acccagatcc gggcaccgtt gatgcggacc
     5221 ccgagcccgg ggatgagcgg gagcaccagc agcagcacgc cgccgaggcc gacggtgtag
     5281 cggacccgtt cgagggtccg cacccgtttg atcaccgcca gggtcacgat gaagccgatg
     5341 acacccacgg cggtccaccc cgcctgctgc gacgccaact tggggttgag ccgggcgatc
     5401 atcacgtagc cgaggccgtt gagcatcacg gcgaccggca ggatcagggg gtcggcccgg
     5461 ggggcgaggc gccgcacacc gatgtgggcg ccgaggaata ggacgaacac cgcgacgacg
     5521 aacgggatga ggttggcggg gagcgccttg gtgcggccga ggctcaccag cgcgaacgcg
     5581 acgccgatga tcaggccggc gagggcgagg agcccggcct cagtggcgcg ccgattgcgc
     5641 ccgtggggcg cgagggcgac ggtcatggaa tggtcgtcac cgtggtctcc gcgcccggtg
     5701 cggtcgtcgt ggtcgtcgtt gtggtggtcg tcgtcgtcgt ggtcgccgca gcggcctgtg
     5761 cctcgagccg ttcgacgtac cggttggcct cggcgaggga gagatcgccg acgccctgtt
     5821 cgaccaagcg ttgctcggcg tcgcgaaggt cgtcgacagc aacgtcggta ggcaggatct
     5881 cggtcttgaa ccacaagatg tcggtgtcgg gtgggccccg gaagatcacg acctgttcgt
     5941 cactcacccc gaccgagtag gtgttcttgt agtaccagtc ggtcgcgaac aatgcggcgg
     6001 cgaacacgag cacgatcagc aggaggaaca cgacggtccg ccaggtgagg cgacggggtc
     6061 gccgcacctt cgccggtgct gccgcctctg ccggggtcgg gcgggcgttc gatgccgaca
     6121 ggttcggatc gtcgacgctg gtctcgtcga agtcggcggc ggtgctgcgc cgggtgatgc
     6181 tctggtcgat cggacgaccc acggcgatcg ccgcggcttc ggcgctgccg gccccgacgt
     6241 cgtgaacgtc gacgacgacg caggtgatgt tgtcgcgccc gccgccggcg ttggccgcgt
     6301 cgatgaggcg acccgcggct tcgaccggat cggtgagctc gaccaggatc agctcgatgt
     6361 cgctgtcgcc cacctcgttg aagaggccgt cgctgcacag gaggaaccgg tcaccgtcgg
     6421 cgacgagcac ctcccaccca tcgacgtcga tgtggggctc gatgccgagc gcgcgggtga
     6481 tgacgttgcg ctgcggatgc acgtcggcct gggcccgcgt gagtcggccc tccctcacca
     6541 gcgtctcgac gaagctgtgg tcttccgaca cctggaacag cgcctgacca cggagcaggt
     6601 agacccgcga gtcgccgacg ttcacgatgg cgacgcgccg gtcgtcgagc ggagcgagcg
     6661 cgcacagcgt cgtgcccatg cccatcagct cgggcgacga acgggcgtgc tcgagcacgt
     6721 cggcgttcgc cgcttgcact gccgccatca gcgactcgag ggtcggcgtc gcggcggact
     6781 gttcgaacgc cacgagcgca cgggccgacg cgacctcacc gccgcggtgg ccgcccatgc
     6841 cgtcggccac ggcgaacagg ccgtcggcga cgagcaggct gtcctcgttc agcgaacgca
     6901 actgcccgac gtcggtgccg gatcccacct cgagacgcat cactgcacct ccaggatcac
     6961 gttgccgacc tgcacccgat caccgaggtg caggcgcgcg atgcgttcga ccttgacgtt
     7021 gttcacgtag gtgccgttgg tcgagccctc gtcctcgacg acccactcgc cgtcgcgtcg
     7081 ttcgaaggca gcgtgacggg tcgagaggaa gctgtcttcg atcaccacgg tcgaatcgcc
     7141 accccgaccg atcgacatcc cctcgcccag gggatattcg atgccaacga ggtgcggtgg
     7201 atcgacggca acgaggcgcg tcggcaccgt gggttcggtc ggttgcttga ccttggcctt
     7261 gcgaggggtc ttcgccggct tcggagcttt cggtgctttc gtcggcgctt ccggtgccag
     7321 acctgccggc acggcagtga cctcggtcca cacggcacgc aggacgcgga agaagaagag
     7381 gtacagcagt accaggaagc agatcttgaa gatcgtgatc agctcgtcgg gcacggcggc
     7441 gcgctcagtc ctgctcgaac ctgatggacg tcgccccgaa gagcacctcg tcgccgtgct
     7501 tgagcagatg ctccgtgacg ttgcgaccat tcaccttggt gccgttggtg gacgccaggt
     7561 cgatgatcgc gaacccaccc tcgacgggac ggaactcggc gtgccgacgc gacgcattct
     7621 ggtcaccgag cacgatcgtg cagtccgagg cgcgtccgac ggacacgacc tcgttgctga
     7681 gcggcacccg atcgccgcca ggaaggatga gtgcggcgat ggccgtgccc ggttcgggtt
     7741 ggcggaaccg ggcgtcgatc gtgaccacgc cgggccgctg ggacggctcg acctcgaaga
     7801 acacttcaac cggaccggcg aagcggtacc gctcttcgct ggcgtgggtc ttcgccaact
     7861 gggtcagctc gttggcgagc gtgtcctcca tgtcggcgag ctgttcgaag tcggcctcgg
     7921 caagcacgag cacgaacgag ttgggtgcca tggtggcgcc gttcacgccg accgtccggt
     7981 ggacgtccat ctcgcggacc aacttccgac cgagctccac cggacgcacg ccactgcgga
     8041 agacccgccc gaagacgccc tccacacctt tctccagctt gcgttcgaag tcggacaaac
     8101 ccacgggccg tacgctatcg gcactctccg gggcggtcgg ggcagaactg gcttgttcgg
     8161 cgcccttcgc cgtcggctat cgtctgaccg ctcacttctt gcgggagtgg cggaattggc
     8221 agacgcgcag gattcaggtt cctgtgtccg aaaggatgtg ggggttcaag tcccccctcc
     8281 cgcacccatg tgatctctca cgacatagga aaccctcgaa ccctcaggtt cgggggtttt
     8341 gt
//
LOCUS       DAOSOA010000122         8320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8529_length_8320_cov_5.178584, whole genome shotgun sequence.
ACCESSION   DAOSOA010000122 DAOSOA010000000
VERSION     DAOSOA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8529_length_8320_cov_5.178584"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PKY13_09045"
     CDS             <1..1124
                     /locus_tag="PKY13_09045"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOT80120.1"
                     /translation="WAAIWGYRHPVLDAAAHEQIDTMSYVMFGGLTHEPAVELATTLV
                     DLAPDGLEHVFLCDSGSVSVEVAIKMCLQHWQSVGRPSKRRLLTWRGGYHGDTFHPMS
                     VCDPEGGMHALWTGVLPEQLFADAPPPGFGAALDEDYVAALRSLVAAHHDELAAIIVE
                     PVVQGAGGMSFHSPEYLRVWRELADEFDVLLVFDEIATGFGRSGELFAADHAGVTPDV
                     MCVGKALTGGYLSMAATLCTTRVAAGISQGEVPVLAHGPTFMGNPLAAAIANASLGLL
                     RSTDWRANVGRIESGLVDGLRGAVDIAGVVDVRVQGAIGVVELDHPVDMAAATAAAVA
                     AGVWLRPFRNLIYTMPPYVTDDDDLQRICSAVRSAALAG"
     gene            complement(1166..1840)
                     /locus_tag="PKY13_09050"
     CDS             complement(1166..1840)
                     /locus_tag="PKY13_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80121.1"
                     /translation="MPRATLDDTRAALLAAGERLVRQRARERSAAVDGALGHLKAGDV
                     AAEAGLSKGMIYHLWPSQEEYRRDLLLHLARATTTESYVATEPTSVVTDYDDADDAIA
                     GIASVTLDRALADEQWFMFMEVSSFVANDGVRGEMAGGQAEALAATARFYEAGLAIWG
                     RRVRAGYTAEDLVLLTRAYSRGVTLQAKVDGGSLFDERPWRGARPRTLYEIGVLALFD
                     ALTETV"
     gene            1942..3156
                     /locus_tag="PKY13_09055"
     CDS             1942..3156
                     /locus_tag="PKY13_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80122.1"
                     /translation="MRTTRPTTRRLATAAVMALTVSLTGCPPGPPPAEPKPQGPSPAS
                     VTAPRPGGSAGAASVSSDGALLAYVHSQLGIDFTSRAEAIAWHRPTDQYRSSRDVFPN
                     ATDTTSPAVSGDGRFVFFSPSDAAESGGARLVRYSTITGAIDQFPIDPVLPGYRITEQ
                     VASNDGSVVALELRGPQGAARSTDVAFWTEARGFDVVEPYGSDVQGSLSDSVGISRNG
                     RYLGASVTLRSGTFLLAVYDSETRSWGSRRVGAPGQWVKELATLDDGSVIFDATTFVT
                     GAAPTSDGVRLWPWAGGSAQQLVPSDVHARGIAASANGRFVAYTESDPDAPPSFSWVG
                     APRLYDRATGVSVPAGRPGDMYDSDGATYPVAVSDDGLTVVLETTEIDLLVVGGPLES
                     PTNRVVVWDRST"
     gene            complement(3162..4595)
                     /locus_tag="PKY13_09060"
     CDS             complement(3162..4595)
                     /locus_tag="PKY13_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT80123.1"
                     /translation="MLLHEVVEFAAVRTPDAVALIFEDRSWTFAELWADVQDVAAWLA
                     ERTEVGDRIAIVSDNRPEVVVLMYAAPMAGVIAMFANTRLVESEINDLIADVTPVLVL
                     RTPELDALLPSGAPRRQFLSPERSSGPVETDTAWIIHTSGTTGRPKGAMLTHRSLMAG
                     VMATALARPVTPDDTYLFPFPLFHVASYGVVHLHFRGRPVVLVPKFDAVPVMAAIEQH
                     RVTTISLAPTMIAMLLDHPRRADFDLTSLRNIFYGASAIPLDVLRRGLVEFGCGFGQG
                     YGMTELSGNAVFLDADDHRRAVTTDPQLLAAAGRVGPLAAVKLADDGEILIRGDQVVP
                     GYWNRPEANEAAFVDGWFRTGDIGRFDDEGYLSIVDRSKDIIVSGGENVASLEVEDVL
                     SEHPAIGAVAVVGVPDERWGERVVAVAVARAGTELDPDEVVAWCNGRLAGFKRPRQLL
                     VVDALPTNASGKVLKREVRVLAAEHLA"
     gene            complement(4606..5133)
                     /locus_tag="PKY13_09065"
     CDS             complement(4606..5133)
                     /locus_tag="PKY13_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOT80124.1"
                     /translation="MIERIDPELQVGERETLNQYLDYGRATLVKIVTGLTKEQLATTV
                     GASDLTLAGLIKHLALVEDAWFVETFLGEPIPEPWTPIDWDADPEWEFRTALDDDPAY
                     LLGLYDAACARARDIVAGAVSLDALSVGVSRRDETLGQPFSLRWIILHMIEETARHLG
                     HADLIRQAIDGATDL"
     gene            5353..7071
                     /gene="leuA"
                     /locus_tag="PKY13_09070"
     CDS             5353..7071
                     /gene="leuA"
                     /locus_tag="PKY13_09070"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOT80125.1"
                     /translation="MPPTPAQPKQMPFEKYQAFVPLELPDRQWPDRKLTKAPRWCAVD
                     LRDGNQALIDPMDPVRKLRMFQTLVAMGFKEIEVGFPSASQTDFDFVRQLIEDDLIPD
                     DVTIQVLTQCREELIERTYESLAGAHSAIVHFYNSTSILQRKVVFGLDRDGITAIAVN
                     AARYCKKLEQQYPETAFRYEYSPESYTGTEIEYAVEICNAVADVIEPTPERPLIMNLP
                     ATVEMYTPNIYADTIEWFLRTVRNRDSIVLSLHPHNDRGCAVAAAELGVLAGADRVEG
                     CLFGNGERTGNVDIVTLAMNLFSQGVDPELDISDIDGLRRTAEYCNRLPVHERHPYVG
                     DLVYTAFSGSHQDAIKKGFAALYAEAEAAGKGHDYDTWAVPYLPIDPAHVGRSYEAVI
                     RVNSQSGKGGVAYIMETEHGFSLPRRLQIEFSKTIQSVVEDTGTEITPGAMWDHFQEI
                     YLPDEPSLRLRSHELVSADDNTTTITAQVTVDGEPATWTGTGNGPLSAFVAAMRDGLG
                     LVLDVVDYQEHSTGNNADASAVAYVETVDEQNSIRWGIARHHNTSTASLLAVLSAAER
                     VRREGR"
     gene            complement(7466..7867)
                     /locus_tag="PKY13_09075"
     CDS             complement(7466..7867)
                     /locus_tag="PKY13_09075"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT80126.1"
                     /translation="MDWNDVTPHLSGLAHIATTGPDGPAVAVVAPMVEGDVVWVQTRR
                     SSHKARNLVVDPQIAMMWQSGSETYLWGTVEVVDDVPTKERLWSLWPYEAAGFFGSPE
                     RPDVVLLRIDPTRATVMAAGPDGPSRLLWHA"
     gene            complement(7880..>8320)
                     /locus_tag="PKY13_09080"
     CDS             complement(7880..>8320)
                     /locus_tag="PKY13_09080"
                     /inference="COORDINATES: protein motif:HMM:NF016212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT80127.1"
                     /translation="ERLAEYGGAYGDLGCHDGLWDIAVRTAHDVGARMALVPLVFEAR
                     GLDVTPGLIERFERHGDVASAEVLRIVLRDEVGHVEVGSRWFAHVCDRRGLEPVAEFE
                     RLVAEYSLLIVPPFNVEARVAAGFAEADLRRWEEAFLGTRGTPR"
BASE COUNT         1354 a         2868 c         2803 g         1295 t
ORIGIN      
        1 ggtgggcggc gatctgggga taccggcacc cggtcctcga tgccgcagcc cacgagcaga
       61 tcgacacgat gagctacgtc atgttcggtg gtctcaccca cgagccggcg gtcgagctgg
      121 cgaccacgct cgtcgacctc gcccccgacg gtctcgaaca cgtcttcctg tgcgactccg
      181 gttcggtctc ggtcgaggtc gccatcaaga tgtgcctcca gcactggcag tcggtcggcc
      241 gtccgtcgaa gcgccggctc ctcacctggc gcggcggcta ccacggcgac accttccacc
      301 cgatgtcggt gtgcgacccc gagggcggta tgcacgcgct ctggaccggt gtcctgcccg
      361 agcagctgtt cgccgacgca cccccgcccg gcttcggagc cgcgctcgac gaggactacg
      421 tggccgcgct ccgctcgctg gtcgcagcgc atcacgacga gctggccgcg atcatcgtcg
      481 aaccggtcgt gcagggcgcc ggtgggatgt cgttccactc gcccgagtac ctccgggtat
      541 ggcgtgaact cgccgacgag ttcgacgtgc tgctcgtgtt cgacgagatc gcgacgggct
      601 tcggacgcag cggcgagctg ttcgccgccg accacgccgg ggtgactccc gacgtgatgt
      661 gtgtcggcaa agcgctgacg ggcggctacc tgtcgatggc ggcgacgctc tgcacgaccc
      721 gcgtcgccgc cggcatttcc cagggcgagg tgcccgtcct cgcccacggc cccacgttca
      781 tgggcaaccc actcgctgcg gcgatcgcga acgcgtcgct gggcctgttg cgatcgaccg
      841 actggcgagc gaacgtgggc cgcatcgagt ccgggctggt cgacgggctc aggggtgccg
      901 tcgacatcgc gggcgtcgtc gacgtccggg tgcagggggc gatcggggtg gtcgagctgg
      961 accatcctgt cgacatggcc gcggccaccg ccgcggcagt agccgccggg gtctggctcc
     1021 gtccgttccg gaacctgatc tacacgatgc ccccgtacgt gaccgacgac gacgatctgc
     1081 agcggatctg ctccgccgtc cggtcggcgg cgctggccgg ctgatccggt ggtcaccgag
     1141 ccaccagcac cacccgccgg cgggatcaca ccgtttcggt gagtgcgtcg aagagggcga
     1201 gcactccgat ctcgtagagc gtgcgcggcc gggcaccacg ccacggccgc tcgtcgaaga
     1261 gtgaaccgcc gtcgactttc gcctgcagtg tgaccccgcg ggagtaggca cgggtgagca
     1321 gcacgaggtc ctcggcggtg tagccggcgc gtacccgtcg tccccagatg gcgaggcccg
     1381 cctcatagaa gcgagcggtc gccgcgaggg cctcggcctg gccgccggcc atctcgccgc
     1441 gaacaccatc gttggcgacg aacgacgaca cctccatgaa catgaaccac tgctcgtcgg
     1501 caagggcccg gtcgagcgtc acggaggcaa ttccggcgat cgcgtcatcg gcatcgtcgt
     1561 agtcggtgac cacgctggtc ggctcggtag cgacgtagga ctctgtcgtg gtggcccggg
     1621 cgaggtggag caggaggtcg cgtcggtact cctcctggga cggccagagg tggtagatca
     1681 tgcccttgct caggccggcc tcggcagcga cgtcgccggc tttgagatgt ccgagggcgc
     1741 cgtcgacggc ggccgagcgc tctcgggctc gttgtcgcac gagccgctcg ccggccgcaa
     1801 gcagcgcggc gcgagtgtcg tcgagtgtcg cgcggggcat ggggcgatgg tatccggcag
     1861 ggaatcaacg accgcttcgt tgactttttg tcagtcaatg agtaccgtgg ggtccaacat
     1921 cgacgttcac cgagggggac catgcgaacg acacgaccga ccacccgacg cctggccacg
     1981 gccgccgtga tggcactcac cgtgtccctg accggttgcc cacccggccc accgccggcc
     2041 gagccgaagc cgcagggacc gtcgccagcg tcggtcacgg ccccccgccc cggcgggtcg
     2101 gcgggcgcgg cgtccgtctc ctccgatggc gcgttgctcg cctacgtgca ctcgcaactc
     2161 ggaatcgact tcacttcgcg tgccgaggcc atcgcatggc atcgtcccac cgaccagtac
     2221 cgcagctcgc gtgacgtctt ccccaacgcc accgacacca cgtccccggc cgtctccggc
     2281 gacggccgct tcgtcttctt ctcgccgtcc gacgctgccg agtcgggcgg agcgcgcctc
     2341 gtgcgttact cgaccatcac cggtgcgatc gaccagttcc cgatcgatcc cgtcctcccc
     2401 ggttaccgga tcaccgagca agtcgcgtcg aacgacgggt ccgtcgtcgc gctcgagctg
     2461 cgcggccccc agggtgcggc ccgatcgacc gacgtcgcgt tctggacgga agcccggggg
     2521 ttcgacgtgg tcgaaccata cgggtccgac gtgcagggca gcctctccga cagcgtgggc
     2581 atctcccgca acggccgcta cctcggggcg agtgtcaccc tccgcagtgg aacgttcctg
     2641 ctcgccgtct acgacagcga gacccgctcc tggggtagcc gccgggtcgg ggcgccggga
     2701 caatgggtca aggagctcgc aactctcgat gacggttccg tcatcttcga cgcgaccacc
     2761 ttcgtcaccg gcgcagcgcc gacctcggac ggcgtcagac tctggccgtg ggccggaggc
     2821 tcggcgcagc agctggtccc gagcgacgtg cacgctcgcg gcatcgcggc gtcagcgaac
     2881 ggtcggttcg tcgcatacac ggaatcggac cccgatgctc cgccgtcgtt ctcgtgggtg
     2941 ggcgcacctc gcctgtacga ccgggccaca ggtgtcagtg ttcccgccgg aaggcccggt
     3001 gacatgtacg actccgacgg agcgacgtac ccagtcgcgg tcagcgatga cggcctgacc
     3061 gtcgtgctcg aaacgacgga gatcgacctc ctcgtggtgg gtggacctct cgagtcgccg
     3121 acgaaccgcg tcgtggtctg ggaccggtcg acctgacgtg gtcaggcgag gtgttcggcg
     3181 gcgaggacac ggacctcccg cttgagcacc ttgcccgacg cgttcgtcgg gagcgcgtcg
     3241 accaccagga gctgacgcgg acgcttgaac ccggcgagcc gaccgttgca ccaggcgacg
     3301 acctcgtcgg gatcgagttc ggtacccgcc cgggcgacag cgacggcgac gacgcgttcg
     3361 ccccatcgct cgtcgggcac gcccacgacc gcgacggcgc cgatggcggg gtgctcgctg
     3421 agcacgtcct cgacctcgag tgacgccacg ttctctccgc ccgacacgat gatgtccttg
     3481 ctgcggtcaa cgatcgacag gtagccctcg tcgtcgaatc gtccgatgtc gccggtgcgg
     3541 aaccagccgt cgacgaacgc agcctcgttg gcctcgggcc ggttccagta cccgggcacg
     3601 acctggtcgc cccggatcag gatctccccg tcgtcggcca gcttcaccgc ggcgagcggc
     3661 ccgacccgtc cggcggccgc gagcagctgc gggtcggtgg tcacggcacg acggtggtcg
     3721 tcggcatcga ggaacaccgc gttgcccgac agctcggtca tgccgtagcc ctgtccgaac
     3781 ccgcagccga actcgacgag gccgcgccgc agcacgtcga gtgggatcgc cgatgcgccg
     3841 tagaagatgt tgcgcagcga cgtcaggtcg aagtcggcgc gccgcgggtg atcgagcagc
     3901 atcgcgatca tcgtgggtgc cagcgagatc gtggtcacac ggtgctgttc gatcgccgcc
     3961 atgaccggca cggcgtcgaa cttcggcacg aggacaacgg ggcggccacg aaagtgcagg
     4021 tgcaccacgc cgtaggacgc gacgtggaag agcgggaacg ggaacaggta cgtgtcgtcg
     4081 ggggtgacag ggcgggccag cgccgtcgcc atcacccccg ccatgagact gcgatgggtg
     4141 agcatcgcgc ccttggggcg gccggtcgtg ccgctggtgt ggatgatcca cgcggtatcg
     4201 gtctcgaccg gcccgctgga gcgctcaggt gacagaaact gtcgcctggg cgctccagaa
     4261 ggcaggaggg cgtcgagttc gggagtgcgg aggacgagca cgggagtgac gtcggcgatc
     4321 aggtcgttga tctcgctctc gaccaaccgg gtgttggcga acatggcgat gaccccagcc
     4381 atcggcgcgg cgtacatgag caccacgacc tcgggacggt tgtccgacac gatggcgatg
     4441 cgatcaccga cctcggtgcg ctccgctagc cacgccgcca cgtcctgcac gtcggcccac
     4501 agctcggcga acgtccacga ccgatcctcg aagatcaggg cgacggcgtc cggcgtgcgg
     4561 accgcggcga actcgacgac ctcgtgcagc agcatcggag ggaggttaga ggtcggtggc
     4621 gccgtcgatc gcctggcgga tgaggtcggc gtggccgagg tgccgagcgg tctcctcgat
     4681 catgtgcagg atgatccacc gcaacgagaa gggctgcccc aacgtctcgt cacggcggct
     4741 gacaccgacc gagagagcgt cgagcgacac ggcccccgcg acgatgtctc gggccctggc
     4801 gcacgcagcg tcgtagaggc cgaggaggta cgcgggatcg tcatcgaggg cggtgcggaa
     4861 ctcccattcg ggatcggcgt cccagtcgat cggcgtccag ggttccggaa tcggctcgcc
     4921 gaggaacgtc tcgacgaacc acgcgtcctc cacgagcgcg aggtgcttga tcagaccggc
     4981 gagtgtgagg tcggacgcgc caacagtcgt ggcgagctgt tccttcgtga gaccggtgac
     5041 gatcttgacc agggtcgctc gtccgtagtc gaggtactgg ttcagggtct cgcgctcgcc
     5101 gacttggagc tcagggtcga tgcgttcgat catccacgac acgctacgac gcggcgccgt
     5161 caccgggtgg tacactcacc accgtgacgg gcaccgctct cctccttctc atgtaggcaa
     5221 gtacccgtcc gggtacttgc cgaagctcct gcgcctccgg gctcgggagc ttctttcgtt
     5281 ttccgcagcg gcggagcgaa cggagacggc gaccgtcacg accatcatca ccaccgagca
     5341 agtgagcaca ccatgcctcc cacgccagcc caaccgaagc agatgccgtt cgagaagtac
     5401 caggccttcg tgccgctcga actgccggat cgccagtggc cggaccgcaa gctcacgaag
     5461 gccccccgtt ggtgcgcggt cgacctccgt gacggcaacc aggcgctgat cgacccgatg
     5521 gaccccgtgc gcaagctgcg gatgttccag accctggtgg ccatgggctt caaggagatc
     5581 gaggtcgggt tcccctcggc gagccagacc gacttcgact tcgtccgcca gctcatcgag
     5641 gacgacctga ttcccgacga cgtcaccatc caggtgctga cgcagtgtcg tgaggaactg
     5701 atcgagcgca cgtacgagtc gctggccggc gcacacagcg cgatcgtcca cttctacaac
     5761 tcgacctcga tcctgcagcg gaaggtggtg ttcggcctcg atcgcgatgg catcacggcg
     5821 atcgccgtca acgctgcccg ctactgcaag aagctcgagc agcagtaccc cgagacggcg
     5881 ttccgctacg agtactcgcc ggagagctac acgggcaccg agatcgagta cgccgtcgag
     5941 atctgcaatg ccgtcgccga tgtcatcgaa ccgactccag agcgtccgct catcatgaac
     6001 ctgccggcga ccgtcgagat gtacacaccg aacatctacg cggacacgat cgagtggttc
     6061 ctgcgcaccg ttcgcaaccg ggattcgatc gtgttgtcgc tgcaccccca caacgaccgc
     6121 gggtgtgccg tggcagccgc cgagctgggt gtgctggcgg gcgccgaccg cgtcgagggc
     6181 tgcctgttcg gcaacggaga gcgcaccggc aacgtcgaca tcgtgacctt ggcgatgaac
     6241 ctgttcagcc agggcgtcga ccccgagctc gacatcagcg acatcgacgg cctgcgccgc
     6301 accgccgagt actgcaaccg cctgccggtg cacgagcgcc atccctacgt cggcgacctc
     6361 gtgtacacgg ccttctcggg ttcccaccag gacgccatca agaaggggtt cgctgcgctg
     6421 tacgccgagg ccgaggcggc cggcaagggc cacgactacg acacgtgggc ggtgccgtac
     6481 ctgccgatcg acccggcgca cgtcggccgt tcctatgagg cggtcatccg cgtcaacagc
     6541 cagtcgggca agggcggcgt ggcctacatc atggagaccg agcacgggtt ctcgctgccg
     6601 cggcgcctgc agatcgagtt ctcgaagacg atccaatcgg tggtcgagga caccggcacc
     6661 gagatcacgc cgggagcgat gtgggaccac ttccaggaga tctacctgcc cgacgagccg
     6721 agccttcggc tccggtccca cgagctcgtc agcgctgacg acaacaccac gacgatcacg
     6781 gcgcaggtga cggtcgacgg tgagcccgcc acctggaccg gcaccggcaa cggtccgctg
     6841 tcggcgttcg tcgcggcgat gcgcgatggc ctgggcctgg tgctcgatgt cgtcgactac
     6901 caggagcact cgacgggcaa caacgccgat gcgtcggccg tcgcctacgt cgagaccgtc
     6961 gacgagcaga actcgatccg ctggggcatc gcccgacacc acaacaccag cacggcgtcg
     7021 ctgctcgcgg tgttgtcggc agcggagcgc gtgcgccgcg agggtcgctg aacctcgggt
     7081 cctcgccagt tccatcgaac aacgacgaag gccggagcgc agagcgctcc ggccttcgtg
     7141 attgctgcac cggtcgacga ccggcggagc gagatcaggc gaacgtgcag ctgatgttcg
     7201 tcggaccctc gaggatcgtc aggacgcacg gacccaccgg aatctggcag gcggcggtgg
     7261 cgaacgggat cgccatgatc atggaagcga gcagaactcg gctggaacga cgcatttcga
     7321 ataccccttt tgtggtgata tgaccttcgt cacacttgtc ggtgtccaag tggcaagtca
     7381 aggaccgtcg caacatttca cgcgtcgccc cttgcccctc cggtgatcat cattcccgcc
     7441 tgtgcccgct ctcagacatc gtcggtcagg cgtgccacag caaccgcgac ggaccgtcgg
     7501 gacccgcggc catgaccgtg gcacgggtcg ggtcgatgcg cagcaggacc acgtcgggtc
     7561 gctccggcga accgaagaat ccggcggcct cgtagggcca caacgaccac agtcgttcct
     7621 tggtcggcac gtcgtcgacg acctcgaccg tgccccacag gtaggtctcg ctcccggact
     7681 gccacatcat cgcgatctgc gggtcgacca cgaggttccg ggccttgtgt gagctgcgtc
     7741 gcgtctgaac ccacacgacg tcgccctcga ccatcggtgc gacgacggcg accgctgggc
     7801 cgtccggccc cgtcgtggcg atgtgagcaa ggccggacag gtgtggggtg acgtcgttcc
     7861 aatccatcgg gtgatcttgt caccgagggg taccgcgtgt gccgaggaac gcctcctccc
     7921 atcggcggag gtcggcctcg gcgaatccgg ccgcgaccct cgcctcgacg ttgaacgggg
     7981 gaacgatcag gagggagtac tccgcgacca gacgctcgaa ctcggcgacc ggctcgaggc
     8041 cacggcggtc gcacacgtgg gcaaaccacc ggctgccgac ctcgacgtgg ccgacctcgt
     8101 cccggagcac gatccggagc acctcggccg acgcgacgtc accatgccgt tcgaaccgct
     8161 cgatgaggcc gggggtgacg tcgaggcccc gcgcctcgaa cacgaggggc acgagggcca
     8221 tacgggcgcc gacgtcatgg gcggttcgca cggcgatgtc ccacaatccg tcgtggcaac
     8281 cgaggtcgcc gtacgccccg ccgtactcgg caagccgttc
//
LOCUS       DAOSOA010000123         8318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8532_length_8318_cov_8.312477, whole genome shotgun sequence.
ACCESSION   DAOSOA010000123 DAOSOA010000000
VERSION     DAOSOA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8532_length_8318_cov_8.312477"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1368
                     /locus_tag="PKY13_09085"
     CDS             121..1368
                     /locus_tag="PKY13_09085"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT80128.1"
                     /translation="MTRSHRLRIALLAAVSMMAAACGGTTSVDAPTTSSTQKAGTLRV
                     PADFPTIQDAVDAAKPGALILIAPGIYNEGVTVETEDLTIRGEDRNTVILDGGDRFEN
                     GVLAFSNGTVVENLTARNFKGNGIMFSGSYGDSQILDGFRGSYLTVYNNGLYGVYAFA
                     AKNGQIDHVYGSGHPDSAVYVGQCNPCNTTVTDSVGELNAVGFQGTNAGGGLIVTTSV
                     WSRNRVGIESTSSTREQVYPQRNATFVANLVSDNNGDGTPQATDAAGVGIALSGGQTN
                     IVDRNVIVGNAASGVIVTSGEGFEPKGNTVKNNTLAGNGVDLVFTTTTGAQDDNCFEN
                     NQFTTSSPTAIEQALVCNAAGTANGTEPTIPVPTHADYRTVPAPGPQQSMPNALTAKA
                     KPARGPLELVYADLTAPDAPEAP"
     gene            1365..1604
                     /locus_tag="PKY13_09090"
     CDS             1365..1604
                     /locus_tag="PKY13_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80129.1"
                     /translation="MTTLAEPAWVVWDRVAAYAVGTTDSPAALAERFGVDEDLVEHEL
                     GERSIHVCADCGWWFDEEADGASDDNFDPVCADCQ"
     gene            1672..2067
                     /locus_tag="PKY13_09095"
     CDS             1672..2067
                     /locus_tag="PKY13_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80130.1"
                     /translation="MSTTREPEIDLAARLAALEAFAEFIPLEDRRSFEGVIELRGSGL
                     LEDEWTLTSAARDSTVLAVTASHWKRRNGQPGTLVVGPPDLDDGVWQVRVTYMPAGEQ
                     LLNWFTVSGPTATAAVVGAIEAFPEGAAR"
     gene            2064..2516
                     /gene="dut"
                     /locus_tag="PKY13_09100"
     CDS             2064..2516
                     /gene="dut"
                     /locus_tag="PKY13_09100"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT80131.1"
                     /translation="MSTEPLEVPIVLLGSGAVVPCVASPGDAGVDLRSTVDLVIDPGG
                     HRAIVPTGVAVAIPAGYAGFIQPRSGLAAKHGVTVLNSPGLIDSGYRGELKVVLVNTD
                     PVEPFVVSVGDRIAQLVIQRVVEPVFVEVTSLDDTVRGAGGFGHTGRR"
     gene            complement(3107..3502)
                     /locus_tag="PKY13_09105"
     CDS             complement(3107..3502)
                     /locus_tag="PKY13_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80132.1"
                     /translation="MTATYTYDVERRALPDHEHRVFNTRSEVEDFLFDVADRHTIPLE
                     LVEWIPARSTCAYASAVTGTVTLPDDPFGRSIITTLHELAHVAAPVDAGHTTEWLYWF
                     TYLLRHEVSITAEAALRFELGIPSATPLR"
     gene            complement(3499..4218)
                     /locus_tag="PKY13_09110"
     CDS             complement(3499..4218)
                     /locus_tag="PKY13_09110"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HOT80133.1"
                     /translation="MSDMTETVRKLLDKAQATDFPEEAEAFFAKAQELITLHGLSQDA
                     IAAAGNVQSDEVTHRDFRFEAPYSAGKLLIAVQIASVFRLKCVRTESGANNVLCAFGT
                     KSKLDDFEMLLTAVNMHATGELLKARPPARENARSFRAAFLQGYASTIGQVLGTADAA
                     ARAAADESFTVEERRGVALVQLSEKERVDEAFTAKFPRTRTTRMSGARSHTGRAAGAA
                     AGERFTGHQNGIGGQRKALSR"
     gene            complement(4299..4553)
                     /locus_tag="PKY13_09115"
     CDS             complement(4299..4553)
                     /locus_tag="PKY13_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80134.1"
                     /translation="MHINTMTDTAAAPNPDSLLLFAELLRRIAAYAIRWGRPDHHEHP
                     SMSLDVSVVNEWTPGHTGVVVEWTPFNSATYSLAFETQKS"
     gene            complement(4584..5417)
                     /locus_tag="PKY13_09120"
     CDS             complement(4584..5417)
                     /locus_tag="PKY13_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80135.1"
                     /translation="MIRTLTEINPAQDHCQRWAARTPTWRHTSDGGFDADRYVVEQID
                     EAVAKAYIVANHYSGSYPASKYRFGLFDGARLAGVLVFGIPASARVLTIPFPGLDPFI
                     ESVELSRLVLDDTVPANGESWFIARCFEEAAARGVRGVVSFADPVPRIVAGRIVHPGH
                     VGIIYQATNAQLCGRSTARTLTLLPDGQVLNDRAAQKVRRQDRGHDYVERHLVQLGAR
                     PLRSGEVPAAWLSAALADVGAVQLRHRGNWRYCFRIGTPAQRRRVHLALPAVPYPKTT
                     D"
     gene            complement(5414..5731)
                     /locus_tag="PKY13_09125"
     CDS             complement(5414..5731)
                     /locus_tag="PKY13_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80136.1"
                     /translation="MTDTAPHQTELPHVSRSGEFPAHMPDTGERVPADPSNFIRDLFL
                     ANGGRINARLVDNAIARHTFTYGAAITEAWESLRTDSYVETDGEEWIWPEQLAYLDWA
                     SHQ"
     gene            5859..6689
                     /locus_tag="PKY13_09130"
     CDS             5859..6689
                     /locus_tag="PKY13_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80137.1"
                     /translation="MSARGYSIPTIKRLWALSGNQCYMPECKVPLAFVDAEHVMGDIA
                     HICAANPKGCRFDPSMTDSERAAFENLLLMCPTHHRLVDKVSCDQWSVDELRKMKAAH
                     ETANHPTSLPEELLGWAARGSLHLLEVAEANDLGHASARRPRQPGPMRPGITASGGVP
                     GRRVQKVQPVPSAPGTGWSVPPHEQSDGFILDSSAVSRDQLGPDPGPDPDGEPPVPGP
                     RFGQARFGQSRFGVNDPGAAVSVMPSPEEAAKHEALAAAAEEDRLGDELSSRRSRPDS
                     "
     gene            complement(6714..7508)
                     /locus_tag="PKY13_09135"
     CDS             complement(6714..7508)
                     /locus_tag="PKY13_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80138.1"
                     /translation="MSAAERITVEHVIDNAEDYADERSTTVEWIIENAEDVADEENAR
                     ATRPEFRMEDRRPMLVLERADLEIVVTHPWVGVAPLDLTCATCGGYGRHPIQLQSDGL
                     FGSKGKCLDCSDGQIELPVGLATMLLADTTGRIHGTWTGRLLPMVDVTPDPPSAPHIY
                     IRPGDNAVYIWNHPHPLAYMPTESLTGTWAPGGHAIHAGTFTSVSTPGGCPACDGEGC
                     IGGWVAEGGTICWLCHGYHRLDGPFRAPGAPNIPCVTTIDLEELAA"
     gene            complement(7505..7711)
                     /locus_tag="PKY13_09140"
     CDS             complement(7505..7711)
                     /locus_tag="PKY13_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80139.1"
                     /translation="MSLQVRRSTYRGTSGWLLCGRPEGAAFPVKVFFENEEVARRERA
                     ALVANPNHQLSFRPVDTTDREGLT"
     gene            complement(7708..>8318)
                     /locus_tag="PKY13_09145"
     CDS             complement(7708..>8318)
                     /locus_tag="PKY13_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80140.1"
                     /translation="GRSDPVPRGHRVTAALILRPHHAALVAAGHPWVGVPGLDLTCPT
                     CGGGWNLKCGRCGASASLPCGMTCDDHQPRRWCPNPNCAEGKASLPDCPSMLLLADTT
                     GNVWGTLHDTEVLDIASLVDGPPIDDDSPVVGVWGISGHAIHVGRYEASVETAHAGRL
                     GPNDLMADLIESVNRHRRRIPFVVPGLPDQPCITTIDLGDAA"
BASE COUNT         1308 a         2606 c         3032 g         1372 t
ORIGIN      
        1 gatgaccgga agctagttcg gcgacagctg gtcgagaagg gcgcaggtca cttttgggca
       61 ggactgctca cgggcggggc ggggggtggg gatcgcgccg gctcggaaca catctccggc
      121 atgactcggt cgcatcgact ccgaatcgcc ctcctcgccg ccgtatcgat gatggccgcc
      181 gcatgcggcg gcaccacctc ggttgacgcg cccaccacct cctcgaccca gaaggctggg
      241 acgcttcggg tgccggcgga cttcccgacg atccaagacg cagtggacgc cgccaagccg
      301 ggggcgctga tcctgatcgc gccggggatc tacaacgagg gcgtcaccgt cgagaccgag
      361 gacctgacga tccgtgggga ggaccgcaat acggtgatcc tcgacggtgg tgaccgcttc
      421 gagaacgggg tgctggcgtt ctccaacggc actgtcgtcg agaacctgac ggcccgcaac
      481 ttcaagggca acggcatcat gttctccggt tcctacggcg acagccagat cctcgacggg
      541 ttccggggca gctacctcac cgtctacaac aacggcctgt acggggtgta cgcgttcgcc
      601 gccaagaacg ggcagatcga ccacgtgtac gggtcgggcc atcccgattc tgcggtgtac
      661 gtcggccagt gcaacccgtg caacacgacg gtcaccgact cggtgggtga gctcaacgcg
      721 gtcgggttcc agggcaccaa cgccggcggc ggcctgatcg tcaccacgtc ggtctggtcc
      781 cgcaaccggg tcgggatcga gtcgacgtcg tcgactcgcg agcaggtcta cccacagcgc
      841 aatgcgacgt tcgtggcgaa cctcgtctcc gacaacaacg gcgacggcac cccgcaggcc
      901 accgacgctg ccggggtcgg catcgctctg tcgggaggtc agaccaacat cgtcgaccgc
      961 aacgtcatcg tcggcaacgc ggcgtcgggc gtgatcgtca ccagcggcga aggcttcgaa
     1021 ccgaagggca acaccgtcaa gaacaacact ctcgccggca acggtgtcga cctggtgttc
     1081 acgaccacca cgggggcaca ggacgacaac tgcttcgaga acaaccagtt cacgacgtcg
     1141 tcgccgacag cgatcgagca ggcgctcgtg tgcaacgcgg cgggcacggc gaacggcacc
     1201 gagccgacga tcccggtccc gacccacgcc gactaccgga cggtgcccgc gcccggcccg
     1261 cagcagtcga tgccgaacgc gttgacggcg aaagcgaagc cggcccgcgg cccgctcgaa
     1321 ctcgtctacg ccgacctcac cgcgccggac gctcccgaag cgccatgacc acgctcgcgg
     1381 agccggcgtg ggtggtgtgg gatcgggtcg cggcgtacgc ggtcggcacg accgattcac
     1441 cggccgcgct ggcggaacgg ttcggggtcg acgaggacct ggtcgaacac gagctcggcg
     1501 agcgatcgat ccacgtctgc gcggactgcg gctggtggtt cgacgaggag gccgacggtg
     1561 cgtccgacga caacttcgac ccggtgtgcg ccgactgcca gtgatcggtt taggcccagg
     1621 cctcgcggca tagagcacca ccaccaggac cagagaggac agacagaagc cgtgagcaca
     1681 acacgtgagc ccgaaatcga tctggcggct cgcctcgctg cgctggaagc gttcgcggag
     1741 ttcatcccgc tcgaggatcg ccgctcgttc gagggggtga tcgaactccg aggttcaggc
     1801 ctgctcgagg acgagtggac gctcacgtcg gcggcacgtg actcgacagt cctggcggtc
     1861 accgcctcgc actggaaacg tcgcaacggc cagccgggca cgctggtggt cggtccaccc
     1921 gacctcgacg acggtgtgtg gcaggtgcgg gtcacgtaca tgcccgccgg cgagcagctg
     1981 ctcaactggt tcacggtgtc gggcccgaca gcgacggccg ctgtggtcgg tgcgatcgag
     2041 gcgttccccg aaggtgccgc ccgatgagca ccgagccgct cgaggtcccg atcgtcttgc
     2101 tgggctcagg cgccgtggtg ccttgcgtgg cgagccctgg cgacgccggc gtcgacctgc
     2161 gctccaccgt cgacctggtg atcgatcccg gcggccatcg tgcgatcgtg ccgaccggtg
     2221 tcgccgtcgc gatcccggcg ggatacgcgg ggttcatcca gccccggagc gggctcgcag
     2281 cgaagcacgg ggtgacggtg ttgaacagtc ccggactgat cgactccggc tatcgcggcg
     2341 aactcaaggt ggtgctcgtc aacacggacc cggtcgaacc gttcgtggtg tcggtggggg
     2401 accggattgc gcagctggtg atccagcggg tggtggagcc agtcttcgtg gaggtgacgt
     2461 cgctggacga cacggtgcgt ggcgccggcg gtttcggcca caccggccgt cgctgagcag
     2521 gccgacgggt cggcctgcac cgcacatcgg gcaatcttcc ccgtgtcagc cctcccggct
     2581 gtctgcggcc gccatccttg gtcgcatgca cacccacgtt gacgtagcga aggacacggc
     2641 ggccgctgtc cggcgccggc gcatcgagct cgggctcacc caagtggagg cggcgggccg
     2701 cagtcgcggt caagtgtcgg tggcgacttg gcgggtactc gagaaagggt tgcgttcttc
     2761 gtttcgggag cgcaccgttg ccggcgtcgg tgtcgctctg gactggccgc ccgacgccct
     2821 cgtgagagtg aacgccggcg agacacatct gccgacggtg tggcgcgatg aaggtggcca
     2881 ccaggcagga gatttgccac caaccgttcg cgcgaggttg gtcgagcagg atcgtcgcat
     2941 cactgagctt gagcaccagg tcgtcgcgtt gcggaggcgt cagcggtacc ggcgcctgtc
     3001 gccaacgatc acgatgagac agcggagcag gccggcgatg ccgatggcgg aactcacgaa
     3061 gtagaccgac aagaccgtct ccacgctgcg atatgtcgcg gcaggcctag cgaagtggtg
     3121 ttgccgacgg gatgccgagc tcgaagcgca gcgctgcttc ggcggtgatc gacacttcgt
     3181 ggcggagcag gtacgtgaac cagtagagcc actcggtggt gtgcccggcg tcgacgggcg
     3241 cggcgacatg ggcgagttcg tggagggtgg tgatgatcga acggccgaac gggtcgtcgg
     3301 ggagcgtcac ggtcccggtg accgcggacg cgtatgcgca ggtcgatcgg gcggggatcc
     3361 attcgacgag ctcgaggggg atcgtgtggc ggtcggcgac atcgaacagg aagtcctcca
     3421 cctcggaccg ggtgttgaac acccggtgct cgtggtcggg gagggcgcgc cgttcgacgt
     3481 cgtaggtgta ggtcgcggtc accgggagag cgccttgcgc tgcccgccaa tcccgttctg
     3541 gtggccggtg aaccgttcgc cggctgcggc acccgcggcg cgaccggtgt ggctgcgagc
     3601 gccgctcatc cgggtcgtcc tggtgcgggg gaacttggcg gtgaacgcct cgtcgacccg
     3661 ctccttctcg gacagctgga cgagggcgac acctcgccgc tcctcgacgg tgaacgactc
     3721 atccgcggca gcacgggctg cggcatcggc ggtgccgagc acctgcccga tggtggaggc
     3781 gtagccctgc aggaaggcgg cgcggaagct gcgggcgttc tcgcgggccg gcggacgggc
     3841 cttgaggagt tcgccggtgg cgtgcatgtt gacggcggtg agcagcatct cgaagtcgtc
     3901 gagcttcgac ttggtaccga aggcgcacag gacgttgttg gcgccggatt cggtgcggac
     3961 acacttcagt cggaacacgg aggcgatctg tacggcgatg aggagcttcc cagcgctgta
     4021 gggggcttcg aaccggaagt cgcggtgagt gacttcgtcg gactgaacgt tgccagcggc
     4081 ggcgatcgca tcctgggaca gtccgtggag agtgatgagc tcctgggcct tcgcgaagaa
     4141 cgcttcggcc tcctcgggga agtcggtggc ctgagccttg tcgagcagct tgcgcacggt
     4201 ttcggtcatg tcggacatcg gtggttctcc tcggttcgtt cgcttgttct ccacctatgt
     4261 ggccgggttc gcgccgatgc ccgtcacctt ctgcgcggct aggacttctg ggtctcgaag
     4321 gcgagggagt aggtcgccga gttgaacggg gtccactcga caacgacccc ggtgtgaccg
     4381 ggcgtccact cgttcaccac ggacacgtcg agactcatcg acgggtgttc gtggtggtcg
     4441 ggccgccccc accgaattgc gtaggccgcg atcctgcgga gcagttcggc gaagaggagc
     4501 aggctgtcgg ggttcggtgc tgcagcggtg tcggtcatgg tgttgatgtg caccggttgg
     4561 ccacggtgcc cgcctctcga cgatcaatcg gtcgtcttgg ggtacggcac ggccggcagg
     4621 gcgagatgga ctcgtcggcg ttgagcgggc gtcccgatgc ggaagcagta gcgccagttc
     4681 ccgcgatgtc ggagctggac ggcgccgaca tcggcgagcg ccgcactgag ccatgccgcg
     4741 ggaacctcac cgcttcgaag gggtcgagct ccgagctgga cgaggtggcg ttccacgtag
     4801 tcgtggccac ggtcctggcg tcgcaccttc tgcgccgctc ggtcgttgag gacctggccg
     4861 tccggaagga gggtgagggt gcgtgccgtc gagcggccgc acagttgggc gttggtcgct
     4921 tggtagatga tgccgacgtg gccggggtgg acgatgcggc cggcgacgat gcgtggcacc
     4981 gggtcggcga acgacacgac accgcgcacg ccgcgagcgg cagcctcttc gaagcatcgg
     5041 gcgatgaacc acgactcgcc gttggcaggc actgtgtcgt cgaggacgag gcgcgagagc
     5101 tcgacggatt cgatgaacgg atcgaggccc gggaacggga tggtaagcac tcgcgccgag
     5161 gccgggatgc cgaacacgag aacgccagcg agcctggccc cgtcgaacag tccgaaccgg
     5221 tacttggatg ccgggtagct gcccgagtag tggttggcga cgatgtacgc cttcgcgacc
     5281 gcctcatcga tctgctcgac gacgtagcgg tccgcatcga atccgccgtc ggaggtgtgc
     5341 cgccaggttg gggtgcgagc ggcccagcgt tggcagtggt cttgggcggg gttgatctcg
     5401 gtgagcgttc ggatcactga tgcgatgccc agtcgaggta ggcaagttgt tcgggccaga
     5461 tccactcctc gccgtcggtt tcgacgtagc tgtcggtccg aagcgactcc cacgcttcgg
     5521 tgatggcggc gccgtaggtg aacgtgtggc gggcgatcgc gttgtcgacg agacgagcgt
     5581 tgatgcgtcc gccgttggcg aggaacaggt cgcggatgaa gttcgagggg tcggctggga
     5641 ctcgttcgcc ggtgtcgggc atgtgcgccg ggaactcgcc gctgcgactg acgtgcggaa
     5701 gttcggtctg gtgcggggcg gtgtcggtca tcgttgctcc tcggtgcggt gtgtcgtcgc
     5761 gacgtatgtg caccggtccg ggccgacccc cacctcggac gacgcacccc gtgtctgcga
     5821 gcagtccggc ggagcatctg ggatctagcg tgctcggggt gagcgcgcgc ggctactcga
     5881 tcccgaccat caagcggttg tgggctctgt cgggtaacca gtgctacatg ccggagtgca
     5941 aggtgccgtt ggcgttcgtc gacgcggagc atgtgatggg cgacatcgct cacatctgtg
     6001 cggcgaaccc gaagggttgc cggttcgatc cgtcgatgac cgacagcgag cgggctgcgt
     6061 tcgagaatct gctgttgatg tgcccgacac atcatcggct cgtcgacaag gtgtcgtgcg
     6121 accaatggtc ggtcgatgaa ctccggaaga tgaaggccgc ccatgagaca gcgaaccatc
     6181 cgacgtcgct gccggaggaa ctgctcgggt gggcggcgcg cggttcgctc cacctgctcg
     6241 aggtggccga ggcgaacgat ctcggtcatg cctcggcccg ccggccgagg cagccgggcc
     6301 cgatgcgtcc cggcatcact gcgtcgggtg gcgtgcccgg tcgtcgggtg cagaaggttc
     6361 aaccggtacc gtcggcccct ggcaccgggt ggtcggtacc acctcacgag cagagcgacg
     6421 ggttcatcct cgacagctcc gcggtcagcc gcgatcagct gggccccgac cccggccccg
     6481 accccgacgg cgagccaccg gtgccggggc cgcgcttcgg tcaggcacgc ttcggacaat
     6541 cgcggttcgg cgtcaacgat cctggggcgg cggtgtcggt gatgccgtcg cccgaagagg
     6601 cagccaagca cgaggctctc gcggcggccg cggaagagga ccgtctcgga gacgagctgt
     6661 cgtcgagacg gtcccgaccg gacagctgag cgcggccgcc ggacgacagg cagttacgcc
     6721 gcgagctcct cgagatcgat ggtggtgacg cacgggatgt tcggtgcgcc cggggcccgg
     6781 aacggtccgt cgagccggtg gtagccgtgg cacagccagc agatggtccc gccttcggcg
     6841 acccacccgc cgatacagcc ttcgccgtcg cacgcggggc atcctcccgg tgtgctgacc
     6901 gaggtgaaag tgccggcgtg gatggcgtga ccgccgggcg cccacgttcc ggtcagcgac
     6961 tcggttggca tgtaggcgag ggggtgcggg tggttccaga tgtagaccgc gttgtcgccg
     7021 ggtcggatgt agatgtgcgg ggccgacggc ggatcagggg tgacgtcgac catgggcagg
     7081 agtcgaccgg tccaggttcc gtggattcgg cctgtggtgt cggcgagcag catcgtggcc
     7141 aagccgaccg gcaactcaat ctggccatcg gagcagtcga gacacttccc cttgctgccg
     7201 aacagcccgt cgctctggag ctggatcgga tgcctcccat accctccgca ggtcgcgcag
     7261 gtgaggtcga gcggagcgac gccgacccag gggtgggtga ctacgatctc gaggtcggct
     7321 cgctcgagga cgagcatggg ccgccgatcc tccatccgga actcggggcg agtcgctcgt
     7381 gcgttctctt cgtcggcgac gtcctccgca ttctcgatga tccactcgac ggtggtcgac
     7441 cgttcgtcgg cgtagtcctc ggcgttgtcg atgacgtgct cgacggtgat ccgctcggcg
     7501 gcgctcacgt gagcccctcg cggtcggtgg tgtcgacggg tcggaacgac agttggtggt
     7561 tcgggttggc gacgagcgcg gctcgctcgc gtcgtgccac ctcctcgttc tcgaagaaca
     7621 ctttgaccgg aaacgctgca ccctccggcc ggccgcatag cagccagccg gaggtgccgc
     7681 ggtaggtcga tcgtcggacc tggaggctca tgctgcgtct ccaagatcga tggtggtgat
     7741 gcacggctgg tcgggcagac cagggactac gaacgggatg cggcgacgat ggcgattcac
     7801 gctctcgatc aggtcggcca tcaggtcatt ggggccgagg cgtccggcgt gagcggtctc
     7861 gacgctcgcc tcgtatcggc cgacgtggat ggcgtggccg ctgatacccc acacgccgac
     7921 gaccggcgag tcatcgtcga taggtggacc gtcgaccagc gacgcgatgt cgagcacttc
     7981 ggtgtcgtgg agagtgcccc acacgttgcc ggtggtgtcg gcgaggagga gcatgctcgg
     8041 gcaatcaggg agcgacgcct tgccctcggc gcagttcggg ttcgggcacc agcgacgcgg
     8101 ctggtggtcg tcacacgtca taccgcacgg gagcgatgct gacgcgccgc atcggccgca
     8161 cttgaggttc caaccgcctc cgcacgtcgg gcaggtgagg tccaggccgg ggacgccgac
     8221 ccacgggtgg cctgcagcga cgagcgcggc gtggtggggg cgcaagatca gggcggcggt
     8281 cactcgatga cctcgcggca caggatcgct tcgaccca
//
LOCUS       DAOSOA010000124         8246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8641_length_8246_cov_5.455134, whole genome shotgun sequence.
ACCESSION   DAOSOA010000124 DAOSOA010000000
VERSION     DAOSOA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8641_length_8246_cov_5.455134"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKY13_09150"
     CDS             <1..592
                     /locus_tag="PKY13_09150"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOT80141.1"
                     /translation="GTKNPDLDAIIRLAPDVVVLDEEENRRPDADALVAAGVPVHVTA
                     VRSVDDVAPALAALAAAVGADPLDAADPLDAADPAPGAGTGASRRAFVPIWRRPWMTI
                     NADTYGASLLARVGWMTVFGDATERYPTVELDDVRRSAPDAALVPTEPYAFDERHCRE
                     LEAELGCPALLVDGKDVFWWGTRTPSALRRLAQVLP"
     gene            589..1497
                     /locus_tag="PKY13_09155"
     CDS             589..1497
                     /locus_tag="PKY13_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HOT80142.1"
                     /translation="MNDLPVIDLRMGDDAATAIDEACRSRGFFVVTGHGVSLDLVADL
                     DACARAFFARPDAEKEPIAMRHGGRAWRGWFPLGGELTSGRPDQKEGLYFGAELPADD
                     HRPMHGPNLFPAEPAELRGLVLRYLDELTALGHRLVALLDRGFGADGRLSSLVADPLV
                     LFRIFGYPAGTEGWGVGEHTDYGLLTILHQDESGGLQVRDGDTWTDVPPLPGSFICNI
                     GDMLEKATGGVYRSTPHRVQNTSSRYRVSMPFFFDPGWDARVERLIEDTPTGVARWDG
                     ADPHLFDGTYGDYVWQKVGSVFPDLR"
     gene            complement(1494..2141)
                     /locus_tag="PKY13_09160"
     CDS             complement(1494..2141)
                     /locus_tag="PKY13_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HOT80143.1"
                     /translation="MATDLDLRERHELCDLMLELGPDVPTLCGEWTAFDLAAHLAIRE
                     RNPLSAPGILVGGPFEAFTRKLMDKEQRRGFEAVVARVRTPPKGPLSVPAIRAAMSLV
                     EYTVHHEDVRRPQGLAPRTDRDDLQTEVWGKVRQLSGLILRKARIGDVALQLEATGAV
                     AGSHRAGKGDRVVTVTGAPVELLLFLYGRQAIAEVEVTGSDVAAVETARTAAFGI"
     assembly_gap    2194..2293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2294..2714
                     /locus_tag="PKY13_09165"
     CDS             <2294..2714
                     /locus_tag="PKY13_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT80144.1"
                     /translation="MRRTYRLDAPPTGYWAGSPTNEAGTGMEPSSTDVEGIGARVEQT
                     GDSAWRVTIAGELDAATAPELAAVLDPLVVTDSPTVVVNLADVTFMDSSGLRTLVRAA
                     NEAKDSGGSLVLARASGAVVRLLEVTGLVDHLPTAEP"
     gene            complement(2764..3246)
                     /locus_tag="PKY13_09170"
     CDS             complement(2764..3246)
                     /locus_tag="PKY13_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT80145.1"
                     /translation="MTKIDGLGPDALTAAIEPIRLLLVEDDPGDVLITREALEQSKLI
                     HHLDVVENGEEAMAYLRHEGRFSDAIAPHLVLLDLNLPRKDGKEVLAEVKGDPNLSHI
                     PVIVLTTSEAEVDVIDTYQLHANAFITKPVDFERFHDVIRRIDDFFLTVVRLPNRSAG
                     "
     gene            complement(3243..4877)
                     /locus_tag="PKY13_09175"
     CDS             complement(3243..4877)
                     /locus_tag="PKY13_09175"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT80146.1"
                     /translation="MSEHPTERRGALGSLGSLANLSMAVAVTVLLVGTIVVIVSFSNL
                     LAARSEVFNRLGPALVQTEKLRTSLLDQETGVRGFAQTHDGRFLEPYEDGKTAEAALT
                     RNLHSLLADEQAPIERRLDDVTGAAATWRRDVAEPIANSPDAANGDDGEQAARMSKLA
                     FDDVRAAVDRLERDVSAGSRAARSDLDTATTSLGVAIGLALAGVTGSGLFGAWLLRRR
                     VVVPIEQLVDQTDSVDLGRFDTEIVVSGPREIEQLATRVDDMRRRLVAELAAAEIARS
                     ELAHQSQSLERSNRDLEQFAYVASHDLQEPLRKVASFCQLLEQRYADELDEKGRLYIE
                     YAVDGAKRMQALISDLLNFSRVGRTTDTFVECDLAAIADTVAESYSAPIIATEAEFHH
                     EHLPVVPGDPSLLAALLQNLLANALKYRRTDVAPRIHLTAEPTDDRWTFTFTDNGIGI
                     DETFRERVFVIFQRLHSRDAFEGTGIGLALCKKIVEFHGGEIWIDAPPRNGGTSVRWT
                     LPSSSAEPSGYIDEHGLAGATVPSDGPGASPSEDLP"
     gene            complement(4874..6067)
                     /locus_tag="PKY13_09180"
     CDS             complement(4874..6067)
                     /locus_tag="PKY13_09180"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT80147.1"
                     /translation="MADTRRTPATTTVAAPRDAAYRVLLIEDDAADTVLFRHGVGEID
                     PDLSITTVASLDEASSHLHDDLDCIVLDLGLPGSIELDALHAVRSTCPEVAIVVLTGW
                     GDESVGTAAVAAGAQDYLIKGEATAPTIARSIRFAIERKRNERSTAMLLRAQLQNVER
                     ERLERALLGTPSFRRADFSWATKYTAARTGVVSGDFIDGVELPDGSIRAIIGDVAGHG
                     PDEAALGVSLRAGWRALVLSDLPADAVLAGLEDLLMTERSTADAYATVCDVTVSPNLR
                     RVEVRSAGHPPPVLAGVGALVDEHLRSPLGMRFGERRWQGTAFELPEVWSIALYTDGL
                     FEIRSDDGEILSIDDVPGAVEQALVDDFIDPDLLLARFAARSTEGWRDDVALAIVSSR
                     VWGSP"
     gene            6263..6706
                     /locus_tag="PKY13_09185"
     CDS             6263..6706
                     /locus_tag="PKY13_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT80148.1"
                     /translation="MDEPPRVDDPTSTWNPRERRMARMEFDASFSSPRRTRVFVGSTA
                     RGWGLGDVGVLAELLVSEVVTNAVEHGSSGGAVEIIALPTGLRVEVTDQSLGLPELRN
                     PSATEPTGRGLSIVDQLSRWWGVERGTDGKKVWFEVDDLDVSRTG"
     gene            6804..7127
                     /locus_tag="PKY13_09190"
     CDS             6804..7127
                     /locus_tag="PKY13_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT80149.1"
                     /translation="MAMDESPSFTVETDEDMGSVVVRATGEIDVATAPQVEGQLLAII
                     DSGSAVVLDLHGVTFIDSSGLRTLVTARQRAEEMSVAFSLAGRSQAVDRLLQVTGLDS
                     VFESS"
     gene            7249..7680
                     /locus_tag="PKY13_09195"
     CDS             7249..7680
                     /locus_tag="PKY13_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80150.1"
                     /translation="MSQHDPEPAFEGSISITIAPDPRLVRPVRLAMGGLASLAGFDVE
                     AIEDLRVAVNELTATLIERGDGSELRLLLTVLDSGAIRIEGSTGLGSVDVTDERFALS
                     DQILSVVADDHGFDTSAGVASAWLERAKVEWLDDDGGEEST"
     gene            7677..>8246
                     /locus_tag="PKY13_09200"
     CDS             7677..>8246
                     /locus_tag="PKY13_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80151.1"
                     /translation="MSGPDRDSGDEPEVVERFRRWKATGNRRLRDQLVVDHQSIAQAC
                     ARRFASRGEPLDDLTQVALLGLVKAVERFDPEKGIPFAGFAVPTITGELRRHFRDATW
                     AVHVPRRAKDLHVRMPATVERLRVSLGRPPTPDELAEELGCSPEDVLDALDAGAAYRS
                     ASTDTPEGFHAASMSQLGTSAGIANEERVL"
BASE COUNT         1205 a         2779 c         2900 g         1262 t
ORIGIN      
        1 cggcaccaag aaccccgatc tcgacgcgat cattcgactc gcccccgacg tcgtggtgct
       61 cgacgaggag gagaaccgtc gccccgacgc cgacgcgctc gtggccgccg gcgtcccggt
      121 gcacgtcacc gccgtccgct ccgtcgacga cgtggccccg gcgctcgccg cgctggccgc
      181 tgcggtcggg gccgacccgc tcgacgccgc cgaccccctc gatgccgccg atccggcgcc
      241 aggcgccggc accggcgcct cccgtcgcgc cttcgttccg atctggcggc ggccgtggat
      301 gacgatcaac gccgacacct acggcgcgtc gcttctcgcc cgcgtcggct ggatgacggt
      361 gttcggcgac gccacagagc ggtaccccac cgtcgaactc gacgacgtcc gccgctcggc
      421 acccgacgcc gccctcgtcc cgaccgagcc ctacgcgttc gacgaacgcc actgccgtga
      481 gctggaggcc gagctcgggt gcccggcgct gctcgtcgac ggcaaggacg tgttctggtg
      541 gggcactcgc acaccgtccg cactccggcg attggcacag gtactcccat gaacgacctc
      601 ccggtcatcg atctccggat gggtgacgac gcagcgaccg ccatcgacga ggcctgcagg
      661 tcgcggggct tcttcgtcgt gacgggacac ggggtctccc tcgacctcgt cgccgacctc
      721 gacgcgtgcg cccgggcgtt cttcgcccgc cccgatgccg agaaggagcc gatcgcgatg
      781 cgccatggcg gtcgggcgtg gcgcggctgg ttcccgttgg ggggcgagct cacgtcgggt
      841 cgtcccgacc agaaggaagg cctctacttc ggggccgaac tgccggccga cgaccatcgc
      901 ccgatgcacg gtccgaacct ctttcccgcc gaaccggccg agctgcgagg gctcgtcctg
      961 cgctacctcg acgagttgac ggccctcggc caccggcttg tcgccctgct cgaccgcgga
     1021 ttcggcgccg acggccgact cagctcgctc gtcgccgatc cgctcgtcct gttccggatc
     1081 ttcgggtacc cggccggcac cgaggggtgg ggagtgggcg agcacaccga ctacgggctg
     1141 ctcacgatcc tccatcagga cgagagcggt ggcttgcagg tccgcgacgg ggacacgtgg
     1201 accgacgtgc cgccgttgcc gggctcattc atctgcaaca tcggcgacat gttggagaag
     1261 gccaccggcg gggtctaccg ctcgacgccc caccgggtgc agaacacgtc gtcgcggtac
     1321 cgggtgtcga tgccgttctt cttcgacccg ggctgggacg cccgggtcga gcggctcatc
     1381 gaggacacac cgacgggggt tgcccggtgg gacggcgccg atccccacct gttcgacggc
     1441 acctacggcg actacgtctg gcagaaggtc ggctcggtgt tcccggacct tcgttagatg
     1501 ccgaaggcgg cggtgcgagc cgtttcgacc gcggcgacat cggagccggt gacctcgacc
     1561 tcggcgatcg cctggcgacc gtagaggaac aacaacagct cgaccggcgc tccggtcacc
     1621 gtgacgaccc ggtcaccctt gccggcgcgg tgcgaaccgg cgacggcacc cgtcgcctcc
     1681 agttgcagcg ccacgtcgcc gatgcgcgcc ttgcgcagga tcagccccga cagctgccgg
     1741 accttgcccc acacctcggt ctgcaggtcg tcgcggtcgg tgcggggcgc aagcccctgg
     1801 ggccgacgga cgtcctcgtg gtgcaccgtg tactcgacga ggctcatcgc cgcccggatc
     1861 gccggcaccg acaaggggcc cttcggcggc gtacggaccc gggcgacgac cgcttcgaat
     1921 ccccggcgct gctccttgtc catcaacttc cgggtgaacg cctcgaacgg gccgcccacc
     1981 aggatgccgg gcgccgagag cgggttgcgc tcgcggatgg cgaggtgtgc cgccagatcg
     2041 aacgcggtcc attcgccaca gagcgtcggt acatcgggac ccagctccag catcaggtca
     2101 cacagctcgt ggcgttcgcg gaggtcgagg tcggtggcca tgcgtcgaac ctaccgtctg
     2161 gacgcgccgc ccacggggta ctgggccggt tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnncatgcgt cgaacctacc gtctggacgc gccgcccacg gggtactggg
     2341 ccggttcgcc gaccaacgag gcaggtacgg gcatggaacc cagcagcacg gacgtcgagg
     2401 gcatcggtgc ccgggtcgag cagaccggcg acagcgcctg gcgcgtcacc atcgccggcg
     2461 aactcgacgc tgcgaccgcg cccgaactcg ccgccgtgct ggacccgctc gtcgtgacgg
     2521 actcgccgac ggtcgtcgtc aacctcgccg acgtcacctt catggactcg tcgggcctgc
     2581 ggaccctcgt gcgcgccgcc aacgaggcga aggactcggg cggttcgctc gtcctcgccc
     2641 gggcatcggg tgcagtcgtc cggctgctcg aggtcaccgg gctggtcgac cacctgccga
     2701 cagcggagcc ctgacctcgg gcggtcacgg gccgcgtgag cgaccggtcg tcgcggcggg
     2761 cggtcagccg gcggagcggt tgggaagtcg cacgacagtc aggaagaagt cgtcgatgcg
     2821 gcggatcacg tcgtggaagc gctcgaaatc gaccggcttg gtgatgaacg cgttcgcgtg
     2881 cagctggtag gtgtcgatca cgtcgacctc ggcctctgac gtggtgagca cgatcacggg
     2941 gatgtggctg aggttcgggt cgcccttgac ctcggccagc acctccttgc cgtccttgcg
     3001 ggggaggttg aggtcgagca gcacgaggtg gggcgcgatc gcgtcggaga atcggccctc
     3061 gtggcggagg tacgccattg cctcctcgcc gttctcgacg acgtcgaggt ggtggatcaa
     3121 cttgctctgc tcgagggcct ctcgggtgat gaggacatca ccgggatcgt cctccaccag
     3181 gagcagccgg atcggctcga tcgcggcagt gagtgcgtcg ggtccgagcc cgtcgatctt
     3241 ggtcatggaa ggtcctcgct cggcgacgcg ccgggaccgt cggaggggac ggtcgctccg
     3301 gcgaggccgt gttcgtcaat gtaccccgat ggttcggcgg acgagctcgg caaggtccat
     3361 ctgaccgaag tccccccatt gcggggaggg gcgtcgatcc agatctcgcc gccgtggaac
     3421 tcgacgatct tcttgcagag cgccaggccg attccggtgc cctcgaacgc gtcacggctg
     3481 tgcaaccgct ggaagatcac gaacacccgc tcgcggaacg tctcgtcgat cccgatgccg
     3541 ttgtcggtga aggtgaacgt ccaccggtcg tcggtgggct cggcggtcag gtggatccga
     3601 ggagcgacgt cggtccggcg gtacttgagg gcgttcgcca acaggttctg gaggagggcg
     3661 gccaggagcg acgggtcgcc ggggacgacc gggaggtgct cgtggtggaa ctccgcctcg
     3721 gtcgcgatga tcggcgctga gtagctctcg gccaccgtgt cggcgatcgc ggcgaggtcg
     3781 cactccacga acgtgtcggt cgtgcgcccg acccgggaga agttgaggag gtcgctgatg
     3841 agcgcctgca tgcgcttggc gccgtcgacg gcgtactcga tgtacaaccg gcccttctcg
     3901 tcgagctcgt cggcgtagcg ctgttcgagg agctggcaga acgacgccac cttgcgaagc
     3961 ggttcctgca ggtcgtgcga cgccacgtag gcgaactgct cgaggtcgcg gttggatcgt
     4021 tcgagcgatt ggctctggtg ggcgagctcc gagcgggcga tctccgccgc cgccagttcg
     4081 gcgaccaacc ggcggcgcat gtcgtcgacg cgggtcgcga gctgttcgat ctcgcggggc
     4141 cccgacacca cgatctcggt gtcgaaccgg ccgagatcga cgctgtcggt ctggtcgacc
     4201 agctgctcga tcgggaccac gacccgacgc cggagcagcc atgcgccgaa caaccccgac
     4261 cccgtgacgc cagcgagggc gagaccgatc gcgacgccga gcgacgtcgt cgcggtgtcg
     4321 aggtcggagc gggcggccct gctgcccgcg gacacgtcgc gttcgaggcg gtcgaccgcg
     4381 gcgcgaacgt cgtcgaacgc gagcttcgac atgcgcgcgg cctgctcgcc gtcgtcgccg
     4441 ttcgctgcgt cgggtgagtt ggcgatgggc tccgcgacgt cccgacgcca cgtcgccgcg
     4501 gccccggtga cgtcgtcgag gcgccgttcg atcggtgcct gctcgtccgc gagcaacgaa
     4561 tgcaggttgc gggtgagggc ggcctcggcg gtcttgccgt cctcgtaggg ctcgaggaac
     4621 cggccgtcgt gggtttgggc gaatccacgc acccccgtct cctggtcgag cagggacgtc
     4681 cggagcttct cggtctgcac gagggccgga cccagacgat tgaagacttc ggagcgtgcc
     4741 gcgaggaggt tggagaagct gacgatgacc acgatcgtgc cgacgagcag cacggtcacc
     4801 gcgacggcca tcgacaggtt ggcgagcgag ccgagcgagc cgagcgcgcc gcgacgctcc
     4861 gtcgggtgtt cactcaaggg gagccccaca cccgcgacga gacgatcgcc agtgccacgt
     4921 cgtcgcgcca tccctccgtg ctgcgggcgg cgaagcgagc gagcaggaga tcggggtcga
     4981 tgaagtcgtc gacgagcgcc tgctcgacgg cgccgggcac gtcgtcgatc gagaggatct
     5041 caccgtcgtc ggagcggatc tcgaagagcc cgtcggtgta gagcgcgatc gaccacacct
     5101 cggggagctc gaaagcggtg ccctgccagc ggcgttcacc gaacctcatg ccgagtggcg
     5161 agcgcaggtg ctcgtcgacc agggcgccga cgccggccag caccggaggt gggtggccgg
     5221 cgctgcgcac ctccacccga cggaggttgg gcgacaccgt gacgtcgcac acggtcgcgt
     5281 acgcgtcggc ggtggaacgt tcggtcatga gcaggtcctc gagaccggcg agtacggcgt
     5341 cggccggcag gtcggacagc acgagcgccc gccagccggc gcggagggag acgccgagcg
     5401 cggcctcgtc ggggccatgg ccggcgacgt cgccgatgat cgcccggatc gatccatccg
     5461 gcagctcgac gccgtcgatg aagtcgccgc tcacgacgcc ggtgcgggca gccgtgtact
     5521 tcgtcgccca ggagaagtcg gcgcgtcgga agctcggggt accgagcagc gcccgctcga
     5581 ggcgttcgcg ctcgacgttc tggagctgag cccgaagcag catcgccgtc gaccgttcgt
     5641 tgcgtttgcg ctcgatcgcg aagcggatgg aacgggcgat ggtgggcgcc gtggcctcac
     5701 ccttgatgag gtagtcctgc gccccggcgg cgacggctgc ggtgccgacc gactcgtcgc
     5761 cccagcccgt gaggacgacg atggcgacct cggggcacgt cgaacggacg gcgtgcaacg
     5821 catcgagctc gatcgaaccg ggcagaccga ggtcgagcac aatgcagtcg agatcgtcgt
     5881 ggagatgcga cgacgcctcg tcgagcgacg cgacggtcgt gatcgacagg tcggggtcga
     5941 tctcgccgac gccatgacgg aacaggacgg tgtcggcggc gtcgtcctcg atgagcagaa
     6001 ctcggtaggc ggcgtcgcgg ggcgcggcaa cggtcgtcgt ggcgggcgtg cggcgcgtgt
     6061 cggccatgtc gtcggtacct cgtcggccag tgggtccgac cacctgcacc ccgtcgcgtt
     6121 cgtgtgacag ggcgcgttcg tgtcggaacg agatcatcgc atcggttacc cccggtgggg
     6181 gatcgccaaa cgtcggggga tcggcttcga cgagcttcgt ccaatccggc tgacatcgtc
     6241 actggccggg tacggagcgc gcgtggacga accaccccga gtcgatgacc cgacctccac
     6301 ctggaatcca cgcgaacgcc ggatggcccg catggagttc gatgcgtcgt tctcgtcccc
     6361 tcgcaggaca cgggtgttcg tcggctccac agcgcggggg tggggcctcg gcgatgtcgg
     6421 tgtgctcgcc gagctcctgg tgagcgaggt cgtgacgaac gccgtggaac acggctcgtc
     6481 gggcggcgcc gtcgagatca tcgccctgcc caccgggctc cgggtcgagg tcactgatca
     6541 atcgctcggg cttcccgaac tccggaatcc gagtgcgacc gagccgacgg gtcgcgggtt
     6601 gtcgatcgtc gaccagctca gccgatggtg gggtgtggaa cggggcactg acggcaagaa
     6661 agtgtggttc gaggtcgacg atctcgacgt gtccaggacc gggtgacggc gcccgccctg
     6721 accgcgcacc cccggatggg gggttgtagg ccggacggct tggacgcatg tcgattcgcg
     6781 acgagcgttg ctacgctccg ctgatggcca tggatgaatc accctcgttc accgtcgaga
     6841 ccgatgagga catgggatcc gtcgtcgttc gggcgaccgg cgagatcgac gtggccaccg
     6901 ccccccaggt cgaggggcag ctgcttgcca tcatcgactc cggctcggcc gttgtactcg
     6961 acctccatgg cgtgacgttc atcgactcct ccggcttgcg cacgctggtc acggcgcgcc
     7021 aacgcgccga ggagatgtcg gtggcgttct ccctcgccgg ccgcagtcag gccgtcgatc
     7081 ggctgctgca ggtcacgggg ctcgacagcg tgttcgagtc cagctgattc cgacgctcgc
     7141 cagcgtggtg gtcgtcgatg tctggccgtt ccccctcgag ggtacgaccg cacgacgccc
     7201 agacccacga ggtacccaat tgctcgtcac cccgttcgag gagaccacgt gagccagcac
     7261 gatcccgagc ccgccttcga gggttcgatc agcatcacca tcgcccccga ccctcgcctc
     7321 gtgcggccgg ttcgactggc gatgggcggg ctggcgtcgc tggccggctt cgacgtcgag
     7381 gcgatcgaag acctccgcgt ggcggtcaac gagctcaccg ccacgctcat tgagcgtggc
     7441 gacgggtcgg aactccggtt gctcctcacc gtgctcgaca gtggagcgat ccgcatcgag
     7501 gggtcgaccg gtctcggcag tgtcgacgtg accgacgagc gcttcgcgtt gagcgaccag
     7561 atcttgtcgg tggtcgccga cgaccacggg ttcgacacgt ccgcgggcgt tgcctcggcg
     7621 tggctcgaac gcgccaaggt cgagtggttg gacgacgatg gcggcgaaga gtcgacgtga
     7681 gtggccctga ccgtgactcg ggcgacgagc ccgaggtggt cgagcggttc cgacggtgga
     7741 aggccaccgg aaaccgccgg ctacgcgacc aactcgtcgt cgaccaccag tcgatcgccc
     7801 aggcctgcgc ccggaggttc gcctcgcggg gcgagcccct cgacgacctg acccaggtcg
     7861 ccctgctcgg cctggtcaag gccgtcgagc gattcgaccc cgagaagggc atcccgttcg
     7921 ccggattcgc cgtgccgacg atcacgggcg agctgcgtcg ccacttccgt gacgcgacgt
     7981 gggccgtgca cgttccgcgg cgcgccaagg acctccacgt ccggatgccg gccaccgtcg
     8041 aacgcctgcg ggtgtccctg gggcgaccgc cgacccccga cgagctggcc gaggagctcg
     8101 ggtgctcacc cgaagacgtg ctcgacgcac tcgacgccgg cgcggcctac cgttcggcct
     8161 cgaccgacac ccccgagggg ttccacgcgg cgtcgatgtc acagttggga acgtcggcgg
     8221 gcatcgcgaa cgaagagcgg gtcctg
//
LOCUS       DAOSOA010000125         8187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8721_length_8187_cov_6.926340, whole genome shotgun sequence.
ACCESSION   DAOSOA010000125 DAOSOA010000000
VERSION     DAOSOA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8187
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8721_length_8187_cov_6.926340"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..1339
                     /locus_tag="PKY13_09205"
     CDS             431..1339
                     /locus_tag="PKY13_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOT80152.1"
                     /translation="MVDVPYLLLDVFTQTQFEGNPLAVVIDPPPLTDGQMANIAREFN
                     LSETIFLGGVGGGRWTTRIFTPAHELPFAGHPTVGGAIALALTGRAQPSPDLTAPTVV
                     LVEGAGDVPVGIVPTGAGLHATFQAPRSPSLDAPIDRAQAAAVLNAVGLEPSDALTAL
                     EPGVWNAGVPITVVPLASVDALGRVAVDTSKLAELVAAGLPPEIYLLAPDSSRSERWR
                     ARMFAPGIGIAEDPATGAAAVAAAGLLATVAVDGEARWLIEQGVEMGRRSRLDLSATV
                     RSGVAQTARLGGSAVLVGEGTLRLAD"
     gene            complement(1340..1753)
                     /locus_tag="PKY13_09210"
     CDS             complement(1340..1753)
                     /locus_tag="PKY13_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HOT80153.1"
                     /translation="MTDMNDFNASIIAEFRANDGKVGGPFEGSPMLLLTTTGAKSGLP
                     RTAPLVSYREGDRLFIFASKAGAPTNPDWFHNISANPTVTVEFGPDTFEATATVVEGS
                     ERDRIYSAQATLMPGFAEYQENTTRVIPVVELVRS"
     gene            complement(1789..3531)
                     /locus_tag="PKY13_09215"
     CDS             complement(1789..3531)
                     /locus_tag="PKY13_09215"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOT80154.1"
                     /translation="MLLVPISVGCLAAIGANRLIAPPDSVVPALLRAAAIVVISSAVV
                     WGADRFARRLLPLAALMQLSLVFPDQAPSRFRSALRSGSSRTLERTLDDTRRHGLAKD
                     PGDAARQVVQLIGAIGDHDRRTRGHSERVRLFADLVGEELKLSKEERQKLQWGALLHD
                     LGKLMVPSDILNKKGKPSAEEWRILEGHPDAGMELIAPLREFLGEWVHAIGGHHEKWD
                     GSGYPAGLRGGQIPRAAAIVAVADSFEVMTAVRSYKKAMPLAEARAELTRCAGTHFSP
                     EVVRAFLSISLGHLRIVAGPLAALAHLPFLGNLAQLPGAAAAAAPAMVSAAAVPAAAM
                     VTAAALTLGTPPKADAVENLTLTQEATAVAAAGAEAPAASGANGDSTTTSAAAANPAI
                     AAAAAGDTATPASTPTTSPTATTAATAAVAPPTTAAAPAGAVSSTTVAAPSTDAARRP
                     ASTTTLPPTTTTSTPPAAAPATSAPTSPYGTFTLLVSTAAIRLPPQSLNGASFARGTN
                     LYVYAPTSAERVEFEFPGGTHRAENYPFDMVTSTGIYASPWIAPATPGTYTITAKTHG
                     SGASKTATATFTIT"
     gene            complement(3709..4146)
                     /locus_tag="PKY13_09220"
     CDS             complement(3709..4146)
                     /locus_tag="PKY13_09220"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HOT80155.1"
                     /translation="MARRFKGVYKVLNVVHERMFTVSNGRITGRAGGMTVVKLTTTGR
                     KSGEPRITMLTSPLVEGDTVVLVASFRGGPNHPAWFLNLRDQPRVQAMRRGADLAMDA
                     EILTGDERVSLWNRVTSLEPRYARYQSRTEREIPLIVLRPAAS"
     gene            4378..>4635
                     /locus_tag="PKY13_09225"
     CDS             4378..>4635
                     /locus_tag="PKY13_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80156.1"
                     /translation="MRWTSGHVRGLLLVLGTSLLVGLSLGSTHVPVGAAAGSVSPVGD
                     PAIRAARGAARLIGGDQVAPSSDTRIDVVPHAAERGLARRTY"
     assembly_gap    4636..4645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5003..6256
                     /locus_tag="PKY13_09230"
     CDS             5003..6256
                     /locus_tag="PKY13_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOT80157.1"
                     /translation="MDSGNLAWVLVCAALVLFMTPGLAFFYGGMVRSKNVLNMLMMNF
                     WCLLVIPVLWAVVGYSLAFSGDGAVIGNFDKAFMRGLDFGGMEPAVLIFLATFASITP
                     ALISGAVADRMKFSAWAIFVPIWSLLVYVPVTYWVFAPNGWLRERGSLDFAGGTAIHV
                     NAGVAALAMILVLGKRRGWPEKVSPPHSMPLVMLGTGILWFGWFGFNAGSELAADGVA
                     VQAFINTFLAAAAAGGCWAFVERLRDGHFTNLGVASGIVAGLVAITPACGFVTSMSAV
                     AIGAIGGAVCSFAIGLKQKFRFDDALDVIGVHLVGGLVGSVLIGFFADPAALGYAPGK
                     GVGADGLLQAEQGLFFGGGAGLLVEQVLANAVAMVFSFVVTYAIAKVLDATIGLRVSE
                     ETEVEGLDIKEHAETAYSSMDRSTI"
     gene            6278..6616
                     /locus_tag="PKY13_09235"
     CDS             6278..6616
                     /locus_tag="PKY13_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT80158.1"
                     /translation="MKLITAIIKPFRLDNVKEALKAVGVEGLTVTEVQGFGRQGGHTE
                     TYRGAEYKIDFVPKVKVEAVVSDSDVEQVVDAIVTGARTDKIGDGKVWVTPAELLVRI
                     RTGERDDDAV"
     gene            complement(6678..>8187)
                     /locus_tag="PKY13_09240"
     CDS             complement(6678..>8187)
                     /locus_tag="PKY13_09240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOT80159.1"
                     /translation="WAVRTDSKWGLKRWFHPADEDSVASVSHIPRGRATQRLASLAVY
                     GSAPAAFAALWWMRSSHLVADTPFWVYAVILAGGAVANLTTSVLVRANPTSSAYIHAR
                     VAASALATAATVYAVGWGSLLLVAYAIGSSELLRTVGPASARPNLLWNCLAIGGGELA
                     IELGIAPSVVDHRLAHVIAISGAVMLAIVTKVLGEAAQATEEAEDLVRARARHFEALI
                     EHASDLIGVVSIDGTIVSVSPAVTPMLGYHPDDVAGQPIARYVEDRYVDGIEDMLRLA
                     VEDVGSASSFELRLRHHDGSTRLVIATLTTPSTDWSDNIVLNVHDITTQRDLESQLRH
                     DARHDPLTGLLNRKAFGEASERSCVRATRSGDTVGMLYIDLDGFKQVNDTFGHDKGDR
                     VLIEAGERLHDCLGEGETLARLGGDEFAVLIDSVDGERPVELAEAILESLGRPIRGLP
                     NDVRVGASIGIALRSSDGIEISTLMQDADAAMYMAKRNGRSRWEINEPVIEV"
BASE COUNT         1176 a         2755 c         2914 g         1332 t
ORIGIN      
        1 ttcacgccgg ccaacaggtc gtcgtccgcg tagaggtgct cgtcgaggcc ggcgatcttc
       61 gccgtcatct cggtcagcac cgttgtgtcg aacatgtgtt cgagtatacg ggaggggtct
      121 gacactcaag cacggcaacg accgtcagcc cggcgacggc cgcaactcga cgagcggttc
      181 caggcgtggc tcctgctcgt gaggcgtcgc gtcgccgagg atggtgccac cgcggcccga
      241 ttcgggcgcg ctggcgcacc atccgcgccg actgcggcga gccctggttc ttctccggcg
      301 gccgcgccaa tcgaacgcac cacctggatt tgagcgcccg gcgtcgagat cgagaggtgc
      361 accatgctcc tggccggtcg ggccggcaaa ccacgccaat cgaacgcatc cacgcaccta
      421 aggtcgggag atggttgacg ttccgtacct cctcctcgac gtcttcaccc agacccagtt
      481 cgagggcaat ccgttggccg tcgtgatcga tccgccgcct ttgaccgacg ggcagatggc
      541 caacatcgcc cgcgagttca acctgtcgga gacgatcttc ctcggcggtg tcggaggcgg
      601 tcgctggacc acgaggatct tcacgccggc gcacgagctc ccgttcgccg ggcaccccac
      661 cgtcggcgga gcgatcgcgc tcgcgctcac cggccgggca cagccgtcgc ccgacctcac
      721 cgcgccgacg gtcgtgttgg tcgagggcgc cggggacgtg ccggtcggga tcgtgccgac
      781 cggggcagga ttgcacgcga ccttccaagc accgcggtcg ccgtccctcg acgctcccat
      841 cgaccgggcg caggccgccg cggtactcaa tgccgtgggg ctggagccgt ccgacgcgct
      901 gaccgcactc gagcccggtg tgtggaacgc cggcgtgccc atcaccgtgg tcccgctggc
      961 gtcggtggat gcgctggggc gcgtcgccgt cgacacatcg aagctcgctg agctggttgc
     1021 cgctggactc cccccggaga tctacctcct cgcgcccgac agctcacgat ccgaacggtg
     1081 gcgggcccgc atgttcgctc ccggcatcgg catcgcggag gacccggcga cgggagcggc
     1141 agcggttgcc gctgcggggc tgctcgccac ggtcgcggtc gacggcgagg cgcgatggtt
     1201 gatcgaacag ggcgtcgaga tgggccgacg cagtcgtctc gacctcagcg cgacggttcg
     1261 gtcgggtgtc gcccagacgg ctcggctcgg ggggtccgcg gtgttggtcg gggagggaac
     1321 cctgcgcctc gccgactgat cagctgcgaa cgagttccac gacggggatg acccgcgtgg
     1381 tgttctcctg gtactcggcg aagcccggca tcagggtcgc ctgcgccgag tagatccggt
     1441 cgcgttcgga accctcgacg acggttgccg tggcctcgaa ggtgtcgggc ccgaactcga
     1501 cggtgacggt cgggttcgcg gagatgttgt ggaaccagtc ggggttggtc ggcgcgcccg
     1561 ccttcgacgc gaagatgaac aggcggtctc cctcccggta ggagacgagc ggtgcggtcc
     1621 ggggcagccc gctcttggcc cccgtggtgg tgagcaggag catcggagag ccctcgaacg
     1681 gcccgccgac cttgccgtcg ttggcgcgga actcggcgat gatggaggcg ttgaagtcgt
     1741 tcatgtcggt catgtcctca acgtagctcc gcgccggccg cgcgcccgtc aggtgatcgt
     1801 gaacgtcgct gtggccgtct tcgacgctcc cgacccgtgg gtcttcgccg tgatcgtgta
     1861 ggtgcccggc gtggcgggcg cgatccacgg cgacgcgtag atcccggtcg aggtcaccat
     1921 gtcgaacggg tagttctcgg cgcgatgggt gccgcccggg aactcgaact cgacgcgctc
     1981 ggcggaggtc ggtgcgtaca cgtacaggtt cgttccccgg gcgaaggaag cgccgttgag
     2041 cgattgcggt ggcagccgga tcgccgccgt cgagaccagc agcgtgaacg tgccgtacgg
     2101 actggtcggc gcgctcgtcg ccggcgcggc agccgggggt gttgacgtcg tggtcgtcgg
     2161 cggcagcgtt gtcgtggacg ccggccggcg ggcggcgtcg gtggacggcg ccgccaccgt
     2221 ggtggaggac acggcgccgg cgggagccgc cgcggtggtc ggaggcgcga cggctgcggt
     2281 tgcggcggtg gtggcggtcg gcgaggtcgt cggcgtcgat gccggggtcg ccgtgtcgcc
     2341 ggcggccgct gccgcgatcg ctgggttcgc cgcggcggcg ctggtcgtgg tcgaatcgcc
     2401 gttcgcgcct gatgccgctg gcgcctcggc tccggcagct gcgacggcgg tggcctcctg
     2461 ggtgagggtc aggttctcga ccgcgtcggc cttgggtggg gtcccgagcg tgagggccgc
     2521 tgcggtcacc attgcggcag cgggaacggc ggcggcgctc accatcgccg gtgccgcggc
     2581 cgcagcggcg ccggggagct gggcgaggtt gccgaggaac gggaggtggg cgagcgccgc
     2641 gagcgggccg gcaacgatcc gcagatgtcc gagcgagatc gacaggaagg cgcggacgac
     2701 ctcgggtgag aagtgggtcc cggcgcaacg cgtcagctcg gcgcgtgcct cggccagtgg
     2761 catcgccttc ttgtacgacc ggacggccgt catcacctcg aagctgtcgg cgacagcgac
     2821 gatggccgca gcgcggggga tctggccgcc acgaaggccc gccgggtacc ccgacccgtc
     2881 ccacttctcg tggtggccgc cgatcgcgtg gacccactcg ccgaggaact cgcgcagcgg
     2941 ggcgatgagc tccatgccgg cgtcgggatg gccctccagg atgcgccact cctcggcgct
     3001 gggcttgccc ttcttgttga ggatgtcgct cggcaccatc agcttgccga ggtcgtggag
     3061 cagcgctccc cactggagct tctgccgctc ctccttggac agcttgagct cctcgcccac
     3121 caggtcggcg aacaggcgaa cgcgctcgga gtggcctcgg gttcggcggt cgtggtcgcc
     3181 gatcgccccg atcagttgaa cgacctggcg ggcggcgtca ccggggtcct tggccaggcc
     3241 gtgcctgcgg gtgtcgtcga gggtgcgctc gagggtgcgg ctgctccccg accgcagggc
     3301 tgaacggaac cgcgacggcg cctggtcggg gaacacgagg gacaattgca tcagtgccgc
     3361 gagcggaagc agccggcggg cgaaccggtc agcgccccag acgaccgcgg acgagatcac
     3421 cacgatcgcg gcggcgcgga gcagcgccgg caccaccgag tcgggcggag cgatcaaccg
     3481 gttggcgccg atcgcagcga ggcatccgac cgagatcggg acgagcagca ccgcgacgcg
     3541 gatgactcgt gccgcgacag gtcgtgcttc ccaggatctc tccggcgcgg acgagttcat
     3601 agacgtggtg atcgacccaa tccacgaact ccttgatcat tggatcggcg tcgtgaccga
     3661 cggccggtcg acgcagttcg acaacgcgtc gggccgctgc cggcagggtc agctcgcggc
     3721 gggacggagc acgatgagcg ggatctcccg ttcggtcctg gactggtacc gggcgtagcg
     3781 gggttcgagg gaggtgaccc ggttccacag cgacacccgc tcgtcgccgg tcaggatctc
     3841 ggcatccatc gcgaggtcgg ctccccggcg catcgcctgc acccgtggct gatcgcggag
     3901 gttcaagaac caggccggat ggttgggacc gccgcggaac gacgcgacga gcaccacggt
     3961 gtcgccctcg acgaggggag acgtcagcat cgtgatgcgt ggttcgccgg acttgcgacc
     4021 ggtggtggtg agcttcacca cggtcatccc gcccgcccga cccgtgatgc gaccgttgga
     4081 gaccgtgaac atgcgctcgt gcaccacgtt cagcaccttg tagacaccct tgaatctcct
     4141 ggccatcggc cggattgtag gtgcctccga cccgtccccc gcggagccgg agcgacgaac
     4201 tcatcgttaa cacgcccgta acaaggccgt tgcagccctg caatcagcgc cctcgaagct
     4261 gtcgcccatc gacgaacacg gcgttaacac ccgagtaaca cagcggaatc ggtggggcaa
     4321 tcaccctccc caagacttcg tgcggtcact gcgggcgagc gaggaaggaa cctgccgatg
     4381 cgatggacat ctggtcatgt gcgcggtctc ctgctcgtcc tcggcaccag cctcctcgtg
     4441 gggttgtccc tcgggtcgac ccatgtgccc gtcggcgcgg cagcggggag cgtgtcaccc
     4501 gtcggtgacc ccgcgatccg ggcggcacgt ggagcggccc gcctcatcgg cggcgaccag
     4561 gtcgcaccgt cgtcggacac ccggatcgac gtcgtccccc acgccgccga acgcgggctg
     4621 gcccgacgga cctacnnnnn nnnnncctcg acggcccggt cactccgact ctcgccattg
     4681 ctgtcgcgtc caccctggtg ttcgcgacgt cgcggcagtc gcgtcgagtc gtccgctgcg
     4741 ccgacctgcc ggcgcgcgcc ccgccgcatc tcgccctcgg ctgagtacgg ccgcgctctg
     4801 gcgcacccac cgaccgaccc ggtcggcacc cggccgacat cggcggcgtc ccaccttcca
     4861 ccggtggccg ctgcggtgat ccccgggccc caactcctcc cgctgaccgg ccacccgtgg
     4921 tcggtccgta ccccaccgct cgggccgcgc cccgagccga ccatccacgg gttgcagccc
     4981 ggaaacgaaa gagagcaagc agatggatag cggaaacctt gcatgggtgc tggtatgtgc
     5041 cgcactcgtg ctgttcatga cgccgggcct ggcgttcttc tacgggggca tggtgcgttc
     5101 caagaacgtg ctcaacatgc tgatgatgaa cttctggtgc ctcctcgtga tcccggtgct
     5161 gtgggccgtg gtcgggtatt cgctggcgtt cagcggcgac ggtgccgtca tcggcaactt
     5221 cgacaaggcg ttcatgcggg gcctcgactt cggcggcatg gagccggcgg tcctcatctt
     5281 cctggcgacg ttcgcctcga tcacccccgc cctcatctcg ggagcggtgg ccgaccgcat
     5341 gaagttctcc gcctgggcga tcttcgtgcc gatctggagc ctgctcgtct acgtgccggt
     5401 cacctactgg gtcttcgccc ccaacggttg gctccgtgag cgggggtcgc tcgacttcgc
     5461 cggtggcacc gccatccatg tcaacgccgg cgtcgctgcg ctcgcgatga tcctggtcct
     5521 cggtaagcgt cgtggctggc ccgagaaggt ctcgccgccc cactccatgc cgctcgtcat
     5581 gctcggcacc ggcatcttgt ggttcggttg gttcggcttc aacgccggct ccgagctcgc
     5641 cgccgacggc gtcgccgtcc aggcgttcat caacaccttc ctcgctgcgg ctgcggccgg
     5701 cggctgctgg gcgttcgtcg agcgtctgcg cgacggccac ttcaccaacc tgggtgtggc
     5761 gtcgggcatc gtggccggac tcgtggccat cacgccggcg tgcgggttcg tgacgtccat
     5821 gtcggcagtc gccatcggtg ccatcggtgg cgccgtgtgc tcgttcgcca tcggcctgaa
     5881 gcagaagttc cgcttcgacg acgcgctcga cgtcatcggc gtgcacctcg tgggtggtct
     5941 ggtcggctcg gttctcatcg gcttcttcgc cgatccggct gctctcggct acgcccccgg
     6001 caagggcgtc ggtgccgacg gtctgctcca ggccgagcag ggactgttct tcggcggcgg
     6061 tgccggcctg ctcgtcgagc aggtgttggc caacgctgtc gctatggtct tctcgttcgt
     6121 cgtcacctac gccattgcga aggtgctcga tgccaccatc gggctgcggg tctccgagga
     6181 gaccgaggtc gaaggcctcg acatcaagga gcacgccgag acggcctaca gctccatgga
     6241 ccgctcgacc atctgacact gacaggatcg atcacacatg aaactcatca cagccatcat
     6301 caagccgttc cgcctcgaca acgtgaagga ggccctgaag gccgtcgggg tcgaagggct
     6361 caccgtcacc gaggttcagg ggttcggtcg ccagggcggc cacaccgaga cctaccgcgg
     6421 tgcggagtac aagatcgact tcgttcccaa ggtcaaggtc gaggccgtcg tgtccgacag
     6481 cgacgtggag caggtcgtcg atgccatcgt gacgggcgca cgcaccgaca agatcggcga
     6541 cggcaaggtg tgggtcaccc ccgccgagtt gctcgttcgc atccgcaccg gcgaacgcga
     6601 cgacgacgcc gtctgatcca ccagatcgat cgtgggtcgg ccgatgcggt acagcgcatc
     6661 gccggcccac gttcgcgtca gacctcgatg acgggctcgt tgatctccca gcgggagcgg
     6721 ccgttgcgct tggccatgta catggcggcg tcggcgtcct gcatgagcgt cgagatctcg
     6781 ataccgtcgc tcgagcggag cgcgatgccg atgctggcgc ccacccgcac gtcgttcggc
     6841 aggccccgga tcgggcgccc caacgactcg aggatcgcct cggccaactc cacgggtcgt
     6901 tcgccgtcca ccgagtcgat cagcacggcg aactcgtcgc cgccgagccg ggccagcgtc
     6961 tcgccctcgc cgaggcagtc gtggaggcgt tcgccggcct cgatgagtac ccggtcgccc
     7021 ttgtcgtggc cgaacgtgtc gttgacctgc ttgaacccgt cgaggtcgat gtagagcatg
     7081 ccgacggtgt cgccgctgcg ggtggcccga acgcaggaac gctccgacgc ctcgccgaac
     7141 gccttgcggt tgaggagacc ggtgagcggg tcgtggcggg cgtcgtgacg gagctgcgat
     7201 tccaggtcgc gttgggtcgt gatgtcgtgc acgttgagca cgatgttgtc cgaccagtcg
     7261 gtactcgggg tggtgagcgt cgcgatgacg agccgggtgc tgccatcgtg gtgccggagg
     7321 cggagctcga acgacgaggc ggaaccgacg tcctcgacgg cgagacggag catgtcctcg
     7381 atgccatcga cgtagcggtc ctcgacgtag cgggcgatcg gctgcccggc gacgtcgtcg
     7441 gggtggtagc cgagcatcgg ggtgacggcg gggctcaccg acacgatcgt gccgtcgatc
     7501 gacaccacgc cgatgaggtc ggacgcgtgc tcgatgagcg cctcgaagtg gcgggcgcgt
     7561 gcccgcacga ggtcctcggc ctcctcggtg gcctgcgcag cttcgcccag caccttcgtg
     7621 acgatggcga gcatgacggc cccggagatc gcaatgacgt gggcgagacg gtggtcgacg
     7681 accgatggtg cgatgccgag ctcgatcgcg agctcaccgc cgccgatcgc caggcagttc
     7741 cacaacaggt tcggtcgggc cgatgccggc cccacggttc gcaggagttc ggaggaaccg
     7801 atcgcgtagg cgacgaggag gagcgacccc cacccgacgg cgtacacggt cgcggcggtc
     7861 gcgagggcgg aggcggcgac gcgggcatgg atgtaggcgc tgctcgtcgg gttggcgcgg
     7921 acgaggacgc tcgtcgtcag gttggcgacg gcgccaccgg cgaggatgac ggcgtacacc
     7981 cagaacgggg tgtcggcgac caggtgggac gagcgcatcc accacaacgc tgcgaacgcg
     8041 gcgggagccg atccgtagac ggcgagtgaa gcgagccgct gtgtggctcg acctcggggg
     8101 atgtgcgaca cggacgccac ggagtcttca tcggctgggt ggaaccacct cttgagaccc
     8161 cacttcgagt cggtccggac ggcccaa
//
LOCUS       DAOSOA010000126         8174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8738_length_8174_cov_4.944944, whole genome shotgun sequence.
ACCESSION   DAOSOA010000126 DAOSOA010000000
VERSION     DAOSOA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8738_length_8174_cov_4.944944"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1545
                     /locus_tag="PKY13_09245"
     CDS             301..1545
                     /locus_tag="PKY13_09245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT80160.1"
                     /translation="MRRLRHIDRSRSDVHDDARSALDDRAALVTGSAMFLLPTLWMVA
                     QVDLGSVPIVTMLLATFAYAAASTVEFEVGSASAVPTEPLLVVMLVLLPPGLVPLVVF
                     AGLGLGSWWIRERRGRSEPLLATACSAAHAVGAAAVVGLIGLGPPGGGSVRMWSVLGL
                     ALVVQGVADLACSWIRNCLGLGVGVSELAHMMWWSFRVDVLLAPLGVAAAIAFPSGPM
                     AVVVATTPLLLLAMLAGDRRRHIDGMLELSSAFHDAADRARRDQLTGLGNRLAWDEAV
                     SRVERRTGPIGVIMLDVDWLKVVNDDYGHEAGDRLLRVVAGVLDAVVPDDATVCRLGG
                     DEFGVLLVDSDLVEADRVAAFIRAGLAACAPVYDTVVSASIGTAASHGPGSVQAALYD
                     ADRDIYRHKAARGRSRSRPRSI"
     gene            1600..>1876
                     /locus_tag="PKY13_09250"
     CDS             1600..>1876
                     /locus_tag="PKY13_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80161.1"
                     /translation="MGFNNPDVPWKEIERALSDRRPAGGNGRHAGDHRTKARDEEHPG
                     DGGDAPAWSRKRLPYEAGVARSRGGVQFGVRHTTTGPGDACPMRPASP"
     assembly_gap    1877..1886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1887..5187
                     /locus_tag="PKY13_09255"
     CDS             <1887..5187
                     /locus_tag="PKY13_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOT80162.1"
                     /translation="PYAELHCHSNFSFLDGASHPEELVEEAARLGLEAIGICDHNGFY
                     GVVRFAEAARVVGIPTVFGAELTLGAASKHGRTVDPDPDGKHLVVMARNPTGYARLAR
                     AISVAQMAGEKNAPRITFEELAVTLRGTSGGSGNIGRGDHLVLTGCRKGTVPAALVAD
                     GPAAAERELRRLVDTFGRENVAVEIWDHGDPLDGPRNDALVEIALRQGCEIVATNNVH
                     YATPDQRRLATALAAVRSRRSLDELDGWIPGGGAAHLRSGAEQARRFARYPGVVDAAA
                     RLGLECAFDLRLVAPQLPPYPCPPGPDGRPLTEMQFLRQLVVEGSERRYGSPGSMKMA
                     KFEAMHGKNPQPQLDHELDLIESLGFAGYFLIVWDIVAFCERNGILCQGRGSAANSAV
                     CYALGITNADAIALGLLFERFLSPERDGPPDIDIDIESNRREEVIQYVYERYDRAHAA
                     QVANVITYRAKSSIRDMARALGYAPGQQDAWSKETDRWGSLPEVGTQAEHDMTVPDVV
                     LEMANEVLHFPRHLGIHSGGMVICDRPVIEVCPTEWGRMENRSVLQWDKEDCAAVGLV
                     KFDLLGLGMLGALHETLDLIEQHHGERIDLSQLPQDDDVYDMICEADTIGVFQIESRA
                     QMATLPRLRPRTFYDLVVEVALIRPGPIQGGSVHPYIRRRNGQEPVTYLHPLLENSLA
                     KTLGVPLFQEQLMQMAIDVAGFTPSEADQLRQAMGSKRSVERMERLRQRLYEGMAVNG
                     IEGDVADQLYEKLVAFASYGFPESHSVSFAYIVYSSCWLKYHYPAAFCAGLLRSQPMG
                     FYSPHSLVQDARRHGVVVRSPDVDASSWQADLEPCDESTGGFAVRLGLNYVRHIGDDL
                     AQELDAGRPYTSIEDVARRGSSAPGGVRRNALEALATAGAFQCFSHPETRRGSIAEPQ
                     GSRRVSGKGAGKDRDRRRDLWTAGAAAASSPDRLAGIVVGLDAPQLPFMTEQEEAVAD
                     LWATGVSADGHPTRFVRPHLDSLGVITAAELRTAEAERNVAVAGVVTHRQRPATAGGT
                     MFINLEDETGLINIVCSLGCWTRYRVVARRAPALLVRGRLERAEGVVNVIAHSIHTLP
                     VAAANPSRDFR"
     gene            5267..5995
                     /locus_tag="PKY13_09260"
     CDS             5267..5995
                     /locus_tag="PKY13_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOT80163.1"
                     /translation="MPIHGSMFQDYQETTGQDPFTLQNKKLLKIYMQYGPVNCRTGSM
                     VAYQGDVRFENTGSGGLNKMLKKAVTGEGVNVMQCTGTGELFVADMASEIQVFYLEND
                     TISCNGASVLAFSGSIEWDIQRIGGGMAGAMAGGLYNVTLRGTGYVALTTKGEPVVLD
                     VSGPPTFADPNAVVFWTSGVQMTIKTDTGGLKSMVRGGTGEMFQMGFSGQGYVMVQPA
                     ENVVTTGGQSAGGGGGLLGGIMGQ"
     gene            6107..7444
                     /locus_tag="PKY13_09265"
     CDS             6107..7444
                     /locus_tag="PKY13_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT80164.1"
                     /translation="MRITSASPRTIGCACFAAAALMVGAAWHGAPAGATTSSAAQPSP
                     SGVATTTTAPATGTGDSIDVLRADADQVRAALADTAAKRSAAQAQLATVRAEAAAAAA
                     AATEAEAAEQVAAAKARRLDTEAKAAAVHAFVGSGRDVPLGMLVDDDTQDAAWESTVL
                     RYQAEQLSDLLARQEAAHDQLKQQRRAREASAAAAAEAEQRSAAQVDALIQLENDQTA
                     LVAGVDERLESALSEAAALAAIDEQAAATLASQEQDLASAAALSVSSPPAATPVTAQP
                     AVRTTTTAPAPTIPPGPSAPATTAPTPTTVPSGPTPSPVPVDTVNVGGFIVARSIGDQ
                     LGRMLAAAQGAGLSLGGSGYRDVNVQIDLRRQHCGTTDYDIWLKPASQCSPPTAIPGR
                     SMHEKGLAIDITCSGVLIRDRASPCFAWLAGNAAGFGFYNLPSEPWHWSTNGN"
     gene            complement(7467..8030)
                     /locus_tag="PKY13_09270"
     CDS             complement(7467..8030)
                     /locus_tag="PKY13_09270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT80165.1"
                     /translation="MPRIDALLAYNCYRWARFQAYAAVLRGRLTTGGRGLLGRRSSLQ
                     VRPGGSMRIGHKFVTGNDVLVASSGSLVLGTNVFVNSFSRIVAHESIEIGDNVVIAAG
                     VSILDHDHRTHVENGALVVENLEFTSEPIRIGSNVWLGDKVTVLKGVTIGDNVIVAAN
                     AVVTKDVAAGTIVGGIPAAKIRDLDLS"
BASE COUNT         1210 a         2806 c         2899 g         1249 t
ORIGIN      
        1 gttgaggaag gggttgctcg actgcatctg ggcctcctgg gccgtcccgg ctctggcgcg
       61 gggattcgtc ggtcagcgta ccgctgcgca cgcgctgcgc ccgggcagcg cccgctgcga
      121 gcggcatccc ctgaccgggt gtgacctcgg aggacgaccg gtttcctcct caaggaatcc
      181 gccgcagttc gccgatgaag ggcggggcgg gcggtcggaa cgatgacggc ggtccgtcga
      241 ggagcgatcc gcgtgcgtca tggacgacca atcagccgac agcccaggtt cgacgcgctc
      301 gtgcgacgcc tccggcacat cgaccgctcg cggtccgatg tccacgacga cgcccggtcg
      361 gcgctcgacg accgggcggc gctcgtcacc gggagcgcca tgttcctcct gccgaccctc
      421 tggatggtgg cgcaggtcga cctggggagc gtcccgatcg tcacgatgct gctggcgacg
      481 ttcgcgtacg cggcggcgtc gacggtcgag ttcgaggtgg gctcggcatc cgcggtcccc
      541 accgaaccgt tgctggtcgt catgttggtg ctgctcccgc cgggcctggt gccgctcgtc
      601 gtcttcgccg ggctcgggct cgggtcgtgg tggatccgcg agcgacgggg gcgctcggag
      661 cccctgctgg cgacggcatg ttcggcggcg cacgccgtcg gtgcggcggc ggtcgtcggc
      721 ctgatcggtc tcggcccgcc cggtggcggg tcggtgcgca tgtggtcggt gttgggcctg
      781 gcgctggtcg tccagggggt ggcggacctg gcctgctcgt ggatccgcaa ctgtctcggt
      841 ctcggcgtcg gcgtgagcga gctggcgcac atgatgtggt ggtccttccg ggtcgacgtg
      901 ctgctggcgc cgctcggggt ggcggcagcc atcgcgttcc cgtcggggcc gatggcggtg
      961 gtggtcgcca cgacgccgct gctcctcttg gcgatgctgg ccggcgaccg gcgtcgtcac
     1021 atcgacggca tgctcgagct gtcgtcggcg ttccacgacg ctgccgatcg ggcgcgtcgt
     1081 gatcagctca ccggtctggg caaccgtctc gcgtgggacg aggccgtgtc ccgggtcgag
     1141 cgccggaccg gcccgatcgg ggtcatcatg ctcgatgtcg actggctcaa ggtcgtgaac
     1201 gacgactacg ggcacgaggc gggggaccgg ctcctgaggg tggtggcggg ggtcctcgac
     1261 gccgtggtgc ccgacgacgc gacggtgtgt cgactgggtg gcgacgagtt cggtgtgctg
     1321 ctggtggata gcgatctcgt cgaggccgat cgggtggcgg cattcattcg ggccgggctg
     1381 gcggcgtgcg cgccggtgta cgacacggtc gtctcggcgt cgatcggcac ggcggcgtcg
     1441 catgggcccg gttcggtgca ggccgccctg tacgacgccg accgtgacat ctaccgccac
     1501 aaggcggcgc gtggccggag tcggtcgcgc cctcgttcga tctgaccctc ccgggaactc
     1561 cggatcgcag agcgaacagg tgttcggtag ggtcggggta tggggttcaa caacccggat
     1621 gttccgtgga aggagatcga gcgagcgctc agtgaccgtc gacccgcggg cggaaacggc
     1681 cgccatgccg gcgatcaccg aaccaaggcc cgggacgagg aacaccccgg cgacggcggt
     1741 gacgccccgg cgtggtcgcg caagcgcctg ccctatgagg ccggcgtcgc ccggtcccgt
     1801 ggtggtgtgc agttcggcgt acggcacacc accacgggcc cgggcgacgc ctgccctatg
     1861 aggccggcgt cgcccgnnnn nnnnnngccg tacgccgaac tgcactgcca ctccaacttc
     1921 agcttcctcg acggtgcatc gcatcccgag gagctggtcg aggaggccgc ccgcttgggg
     1981 ctcgaggcca tcggcatctg cgaccacaac ggcttctacg gggtcgtgcg gttcgccgag
     2041 gcggcccggg tggtgggcat ccccaccgtg ttcggggcgg agctgacgct cggggcagcc
     2101 tcgaagcacg ggcgcacggt cgatcccgat cccgacggca agcacctggt cgtgatggcc
     2161 cgcaatccca cggggtacgc ccggctggcc cgggccatca gcgtcgccca gatggccggc
     2221 gagaagaacg ccccgcgcat cacgttcgag gagttggcgg tgaccctgcg gggcacgtcg
     2281 ggtggctcgg gcaacatcgg tcggggcgac cacctggtgc tcacggggtg ccgcaagggc
     2341 acggtgccag cagcgctcgt cgccgacggt ccggcggcgg ccgaacgcga gctccgacgg
     2401 ctcgtcgaca cgttcgggcg ggagaacgtg gccgtcgaga tctgggatca cggcgatccg
     2461 ctcgacggtc cccgcaacga cgccctcgtc gagatcgccc tgcggcaggg gtgcgagatc
     2521 gtggcgacga acaacgtcca ctacgccacc cccgaccagc ggcggctggc gacggcgctg
     2581 gcggcggtcc ggtctcgtcg cagcctcgac gagctcgacg ggtggatccc gggcggggga
     2641 gcggcccacc tccggtccgg ggccgagcag gcccggcggt tcgcgaggta tcccggcgtg
     2701 gtcgacgccg cggcgcgcct cggtctggag tgcgcgttcg acctccggct ggtggcgccg
     2761 cagctgccgc cgtatccgtg tccccccggc cctgacggtc gtccgctcac cgagatgcag
     2821 ttcctccgtc aactggtggt cgagggttcg gaacgccgct acggatcacc aggctccatg
     2881 aaaatggcca agttcgaggc catgcacggc aagaaccctc aacctcaact cgaccatgag
     2941 ctcgacctga tcgagtcgct cgggttcgcc gggtacttcc tgatcgtgtg ggacatcgtc
     3001 gcgttctgcg agcgcaacgg catcctctgc caaggtcgcg gctcggccgc gaactcggcg
     3061 gtctgctacg cgctgggcat caccaacgcc gatgccatcg ccctcggact cctgttcgag
     3121 cggttcctgt cgcccgagcg cgacgggccg cccgacatcg acatcgacat cgaatcgaac
     3181 cggcgcgaag aggtcatcca gtacgtctac gagcggtacg accgcgccca cgcggcccag
     3241 gtcgccaacg tgatcaccta ccgggcgaag tcgtcgatcc gcgacatggc ccgggcgctg
     3301 gggtacgccc cgggtcagca ggatgcctgg tcgaaggaga ccgaccggtg gggcagcctc
     3361 cccgaggtcg gcacccaggc cgagcacgac atgacggttc ccgacgtcgt gttggagatg
     3421 gcgaacgagg tgctgcactt cccccgtcat ctcggcatcc attcgggcgg catggtcatc
     3481 tgcgaccggc ccgtcatcga ggtgtgtccc accgagtggg gtcgaatgga gaaccggtcg
     3541 gtcctgcagt gggacaagga ggactgcgct gcggtcgggc tcgtgaagtt cgacctgctg
     3601 ggcctcggca tgttgggtgc cctccacgag acgctcgacc tcatcgaaca gcaccacggc
     3661 gagcgcatcg acctgtcgca gctcccccag gacgacgacg tgtacgacat gatctgcgag
     3721 gccgacacca tcggggtgtt ccagatcgag tcacgggccc agatggccac gctgccccgc
     3781 ctgcggcccc gcacgttcta cgacctggtg gtcgaggtgg cgctcatccg ccccggcccc
     3841 atccagggtg ggtcggtgca cccctacatc cggcggcgca acggccagga acccgtcacc
     3901 tacctccacc cgctcctcga gaactccctg gccaagaccc tcggggtgcc gctgttccag
     3961 gagcagctca tgcagatggc gatcgacgtc gccggcttca ccccgtcgga ggccgaccag
     4021 ctgcgccagg cgatgggctc gaagcgttcg gtggaacgta tggaacgcct ccggcagcgg
     4081 ctctacgagg ggatggccgt caacggcatc gagggcgacg tcgccgatca gctctacgag
     4141 aagctcgtcg cgttcgccag ctacggcttt cccgagagcc actcggtgag cttcgcctac
     4201 atcgtgtact cgtcgtgctg gctcaagtac cactacccgg ccgcgttctg tgccggcctg
     4261 ctgcgttccc aacccatggg cttctactcg ccgcactcgc tggtgcagga cgcccgccgt
     4321 cacggggtgg tcgttcgcag tcccgacgtc gacgcgtcga gctggcaggc cgatctcgag
     4381 ccgtgcgacg aatccaccgg tgggttcgcc gtccggctcg gcctcaacta cgtccgccac
     4441 atcggcgacg atctcgccca ggaactcgac gccggccggc cctatacctc catcgaggac
     4501 gtcgcccgcc gggggagcag cgcccccggc ggggtccggc gcaacgcgct cgaggccctg
     4561 gccactgccg gggcgttcca atgcttctcc caccccgaaa cgcgtcgcgg atcaattgcc
     4621 gaaccgcaag gctcgcgacg cgtttccggg aagggagcgg ggaaggatcg ggatcgacgg
     4681 cgcgacctgt ggaccgccgg ggcagcggcg gcgagcagcc ccgaccgtct ggccggcatc
     4741 gtcgtcggcc tcgacgcccc gcagctgccg ttcatgaccg agcaggagga ggcggtcgcc
     4801 gacctgtggg caaccggcgt gtccgccgac ggccacccca cccggttcgt ccgtccgcac
     4861 ctcgactcgt tgggggtgat caccgccgcc gaactgcgca ccgccgaggc cgaacgcaac
     4921 gtcgccgtcg ccggggtggt gacccaccgg cagcgcccgg cgaccgccgg gggcacgatg
     4981 ttcatcaacc tcgaggacga gacgggcctc atcaacatcg tctgttccct cgggtgctgg
     5041 acccgctacc gcgtcgtcgc tcggcgggcc ccggccctcc tcgtccgggg tcggctcgaa
     5101 cgggccgagg gagtggtcaa cgtcatcgcg cacagcatcc acacgctgcc ggtggcggcg
     5161 gcgaacccgt cgcgcgattt ccgctgattc gtgcggattc ccgccggaac gggggacccc
     5221 gtggttacca ttcctggcat ccgcccccgc ctcgtaggag accgccatgc ccatccacgg
     5281 ttcgatgttc caggactatc aggagacgac ggggcaggac cccttcaccc tgcagaacaa
     5341 gaagctcctc aagatctaca tgcagtacgg cccggtgaac tgccgcaccg gttcgatggt
     5401 cgcctaccaa ggcgacgtcc ggttcgagaa caccggctcg ggtggtctca acaagatgct
     5461 caagaaggcc gtgaccggtg agggtgtcaa cgtgatgcag tgcaccggca ccggtgagct
     5521 gttcgtcgcc gacatggcgt cggagatcca ggtcttctac ctggagaacg acaccatctc
     5581 ctgcaacggc gccagtgtgc tcgcgttctc cggctcgatc gagtgggaca tccagcgcat
     5641 cggtggcggc atggccggtg ccatggctgg tggtttgtac aacgtgacgc tgcgcggcac
     5701 gggctacgtg gcgctcacga ccaagggcga gcccgtggtg ctcgacgtct ccggcccccc
     5761 gaccttcgcc gatcccaacg ccgtggtgtt ctggacgtcg ggtgtccaga tgaccatcaa
     5821 gaccgacacc ggcgggctca agtcgatggt ccgtggcggc accggtgaga tgttccagat
     5881 gggcttcagc ggccagggct acgtgatggt gcagccggcc gagaacgtcg tgacgaccgg
     5941 tggccagtct gccgggggtg gtggggggct gctcggcggc atcatgggtc agtagtcggc
     6001 acgaccgagg ggtgatgccc cgcagtccgg cgatctgctg aactgtgacg gcgtctcggc
     6061 acacgagacg tcaagttggg ttcccgaaag gccgatactc aggcgagtgc gaatcacgtc
     6121 cgcttcccca cgaacgatcg gatgcgcctg cttcgccgcg gccgcactga tggtgggcgc
     6181 ggcgtggcac ggcgccccgg caggagcgac gacctcctcg gcggcccaac cgtcgccgag
     6241 cggggtcgcg accaccacga ccgccccggc caccgggacc ggcgactcga tcgacgtgct
     6301 ccgcgccgat gccgaccagg tgcgcgccgc gcttgcggac acggcggcca agcgcagtgc
     6361 ggcccaggcg cagctggcga cggtccgggc ggaggcggcc gcggccgcag cggcggcgac
     6421 cgaggccgag gccgcggagc aggtggccgc ggcgaaggcg cgccggctcg acaccgaagc
     6481 gaaggcggcc gccgtccacg cgttcgtcgg gagcggtcgt gatgtcccgc tcggcatgtt
     6541 ggtcgacgac gacacccagg acgctgcgtg ggaatccacg gtgctgcgct atcaggccga
     6601 gcagctcagc gatctgctgg cccgccagga ggcggcgcac gaccagctga agcagcaacg
     6661 gcgtgcacgt gaggcgtcgg cagcagccgc cgccgaggcc gagcagcgtt ctgccgccca
     6721 ggtggacgcg ctgatccagc tcgagaacga ccagaccgcg ctcgtcgccg gtgtcgacga
     6781 gcggctggag tcggcgctgt cggaggcggc cgccctcgca gcgatcgacg agcaggcagc
     6841 ggcgacactc gcgtcccagg agcaggatct ggcgtcggcc gcagcgctga gcgtcagctc
     6901 gccgcccgca gcgacgccgg tgacggcgca accggccgtg aggacgacga cgacggcgcc
     6961 cgctcccacg atccctcccg gcccgtccgc gccggcgacg accgctccca cgccgaccac
     7021 ggtgccgtcg ggcccgaccc cgtcgccggt acccgtcgac accgtcaatg tgggcggctt
     7081 catcgtggcc cgctcgatcg gcgaccagtt ggggcggatg ctcgccgcgg cgcaaggcgc
     7141 cgggctgtcg ctgggcggca gcgggtaccg cgacgtcaac gtccagatcg acctccgccg
     7201 ccagcactgc ggcaccaccg actacgacat ctggctgaag ccggcgtcgc agtgctcacc
     7261 gccgacggcg atccccggcc gctcgatgca cgagaagggg ttggccatcg acatcacgtg
     7321 ttcaggtgtg ctgatccgcg accgtgccag cccgtgcttc gcgtggctcg cggggaatgc
     7381 cgccggattc gggttctaca acctgccgtc ggagccgtgg cactggagca cgaacggcaa
     7441 ctgacgctgg tcggctctgg agcggatcaa gacaggtcga ggtcgcggat cttggcagcg
     7501 gggatgccac cgacgatcgt gccggcggcg acgtccttcg tgacgacggc attggcggcg
     7561 acgatcacgt tgtcgccgat cgtgacgccc ttgagcacgg tcaccttgtc gccgagccac
     7621 acgttggagc cgatccggat cggttcggac gtgaactcga ggttctcgac gacgagggcg
     7681 ccgttctcga cgtgggtgcg gtggtcgtgg tcgaggatcg acacaccggc ggcgatgacc
     7741 acgttgtcgc cgatctcgat cgactcgtgg gcgacgatgc gcgagaagct gttgacgaac
     7801 acgttggtgc cgaggacgag cgagccgctc gacgcgacga gcacgtcgtt gccggtcacg
     7861 aacttgtgcc cgatccgcat cgatccaccg ggccgcacct gaagcgagga acgacggccc
     7921 aacaggccgc gcccgccggt cgtcagccga ccgcgcaaca ctgcggcgta ggcctggaac
     7981 cgagcccagc gatagcagtt gtaggcgagg agggcatcga ttcgtggcat agcggatcca
     8041 tcgtggcaga aattgccgtc gatccggttc tccccggcgc tagaacttcc ctctgtgaat
     8101 tcccgtgagt gacctcgaac ccgtcgacat cccgaccgag ctcgctgcgc tcgaccgggc
     8161 ccgggatgcg acga
//
LOCUS       DAOSOA010000127         8164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8759_length_8164_cov_6.984092, whole genome shotgun sequence.
ACCESSION   DAOSOA010000127 DAOSOA010000000
VERSION     DAOSOA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8759_length_8164_cov_6.984092"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKY13_09275"
     CDS             complement(<1..202)
                     /locus_tag="PKY13_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80166.1"
                     /translation="MADLQPPELAELVDFPERPRTENWSLRAALTRYAQPQPMRVSRL
                     LDVVRRIEAAFAADLAVLRSDGP"
     gene            complement(240..467)
                     /locus_tag="PKY13_09280"
     CDS             complement(240..467)
                     /locus_tag="PKY13_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80167.1"
                     /translation="MTRNRKMVVLTATLLVVAVVVSSCELSLNNVDCSGIDQPDMGGL
                     VCLGAKVVALQAGAALILAVLAALLGGAFPG"
     gene            complement(622..697)
                     /locus_tag="PKY13_09285"
     tRNA            complement(622..697)
                     /locus_tag="PKY13_09285"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Glu,seq:ttc)
     gene            816..2291
                     /locus_tag="PKY13_09290"
     CDS             816..2291
                     /locus_tag="PKY13_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80168.1"
                     /translation="MYPDPYGAYWPPPPPSGPSVRTRVGLAMAAALALVGASAVAGAV
                     VFDGGPIYPERWDPRVAEIARFVEEERGHTYRHPVRVYFLAPDRYREIAGGGPDEAAP
                     TDEEREYSENTVAEYRALGLMEGDPDLLAANETMQDSGTLAFYSPEDDVVNVRGTDIT
                     PAMRVTLAHELTHALQDQYFDLSPVGDAATADESGAARAIVEGDAVSVEDAYVAAMSD
                     EDRADYERESSSDSDAADTDLGDVPAVLQVLFGSYYAVGNAFVEFWQADPSGVVDREN
                     IDAVLRRLPEGSHQLFEPARYLDFTEPQVVEPPVDESSSDEFERSSHGVDLLYVMLAE
                     RIDPLTALRATDGWWGDAYVAAVGDNDKMCVSSRVAFGAEEDVTEFRDALNEWSTTMP
                     ADAEVAIRNDDEPLQVTFTTCDPGPLAEMNLTDQAGPALAFPVTRLRAAADAVSNGRT
                     RESALCYGQALIDRLTLTDLQSDERTPAITAALEEAAAACP"
     gene            complement(2324..4183)
                     /locus_tag="PKY13_09295"
     CDS             complement(2324..4183)
                     /locus_tag="PKY13_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HOT80169.1"
                     /translation="MKALTLGALGVVFGDIGTSPLYALRESFHEHHGRQLVVDEANVL
                     GLLSLVFWALVIIISIKYLLFVMRADNGGEGGILALTALVRPSGSLLGGSTRWLVLLG
                     LFGASLLYGDGMITPAISVLSAVEGVKVVTTELDDYIIPIAIVILVALFAVQKRGTAA
                     VGKVFGPIMVVWFLVLGVLGVSHIADDLSILRALSPHFAVAFMVHNGILGFLVLGSVF
                     LVVTGGEALYADMGHFGRNPIKFAWYGLVLPALLLNYFGQGAMMLSNPGKRFENPFYE
                     MAPRWALWPLVILATCATVIASQALISGAFSLTRQAVQLGYLPRVKITHTSESEEGQI
                     YVPAINWLLLAACVGLVLGFKTSSNLAAAYGLAVTATMAITTILFYRVARTRFGWSTA
                     KTLPLCTLFMVMDLGFLGANLPKIPDGGWVPLVIGGGFLIVLTTWFAGRKITAERIEH
                     RNLQLLDFIENLGDTPILRGPGVGVYLGSNPDIVPQALSSHLRHASVLPAAVVVLAVK
                     VENRPHVFGDERLTYDDLGNGVHRLVHHAGFSDDLDIPLLLATDGARLTGLDFSAATF
                     VLGRETLRVTDRPGMAKWRERLFVTMLRNATTADVFFKLPPDRTIELGVQVEL"
     gene            complement(4278..5180)
                     /locus_tag="PKY13_09300"
     CDS             complement(4278..5180)
                     /locus_tag="PKY13_09300"
                     /inference="COORDINATES: protein motif:HMM:NF020989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HOT80170.1"
                     /translation="MPDAWSGADERDLDDEPPWIVLGEDDEFTIAVPAERLALMVARL
                     EQAADAFSTELTVEVDSPTGRRLLERTEIARLIDDASSTCLDIVRHSPATLDYLELVD
                     LTQSSELLTTIWSLSLQADDPDGADLERELAWVAAIGKLAADCEDMFLTRIRPRDDGT
                     FTLRWSLVDRALVREAIAELRSLMSSDDPAISRLFPSAYGSDAERNAGWDVLMRGELI
                     DKRLAALQVTEELMTRRSCTEDELNAFMRCLNDARLVLGTQLDVDESGFPADPDGATR
                     TDRIVYESLTHLLGRTVEALSGGL"
     gene            complement(5186..5488)
                     /gene="clpS"
                     /locus_tag="PKY13_09305"
     CDS             complement(5186..5488)
                     /gene="clpS"
                     /locus_tag="PKY13_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOT80171.1"
                     /translation="MSEPTFEPSSPVGVLEPDETVDVERPWVVLVWNDPINLMPYVTF
                     VLRKLFGYSESKAHALMLEVHLNGRAAVSSGTRESAERDVFRLHEHGLWATMQQDD"
     gene            complement(5499..6488)
                     /locus_tag="PKY13_09310"
     CDS             complement(5499..6488)
                     /locus_tag="PKY13_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOT80172.1"
                     /translation="MLLADRFRPTPRQYVRITQIALAGLATIIVTGAAVRLTGSGLGC
                     TDWPLCEQGKPVAPLEFHPMVEFVNRLITGLVSASVALAVLGSMRRRPRRRDLTRWSW
                     FLVAGVVAQIIIGAFVTLSELKYSVVAVHFLVSMVLVWAAVVLMHRAADEDLDRPEVL
                     DGTVAPPAPRRWTTEAKVLVGLGTAVLVSGTMVTSAGKHPGALPDSAGVEHVVERLPF
                     DVSSVVRIHSILVWLLCLTTIVTALRLRDRTPAAFQTCTRLLVVVVAQGALGYVQYFS
                     DVPALMVGFHVAGAIVVWVMALRVGFVTADAAPSPSAVHETPRRDRAGAPVGR"
     gene            6689..7573
                     /locus_tag="PKY13_09315"
     CDS             6689..7573
                     /locus_tag="PKY13_09315"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOT80173.1"
                     /translation="MTHGVMAKVGGYVALTKPRIIELLLVSTVPTMIVAERGIPSLWL
                     MFATVVGGTLSAGGANAINMYVDRDIDALMDRTKGRPLVTGVMSPRAALVFAVGIEIV
                     SFAFLWAFVNLLSAVLAVSACLFYVFVYTLWLKRTSERNIVIGGAAGAAPVLIGWSAV
                     TNSLSWAPVVLFAVMFYWTPPHFWALAIKYKDDYSKADVPMLPSVAAYETVTRKIIAY
                     TVVVVALTFVFGPVADLGAVYLVSAGVLGAVFLALAVKVHRTESAAAAMKLFTYSITY
                     ITLLFGAMAVDVLVKHGA"
     gene            7736..>8164
                     /locus_tag="PKY13_09320"
     CDS             7736..>8164
                     /locus_tag="PKY13_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80174.1"
                     /translation="MALTETRPQAAGAASRPDGGAPERTQVTAVEKVLGTGDHKTIGR
                     IFIGASLLFVMSDLVMAALVNLQASAGGDFLEATIANRLSLNHPLGLLLCGALPLMLG
                     LAIYLVPLQVGSPTIAFPRAAAASLWGWLLGTVLFVVALLI"
BASE COUNT         1325 a         2728 c         2858 g         1253 t
ORIGIN      
        1 ccgggccgtc ggatcggagc acggcgagat cagcggcgaa cgcggcttcg atccggcgaa
       61 cgacgtcgag cagccggctc acccgcatcg gttgcggctg cgcgtagcgg gtcaacgcgg
      121 cgcgcaggct ccagttctcc gtccgcggcc gctccgggaa gtcgaccagt tcggcgagct
      181 cgggcggttg tagatcggcc accccgagat catggcagcc acgctgccgc cgagccggat
      241 cagccgggga atgccccgcc gagcaatgcg gcgagcactg cgaggatcag cgccgctccg
      301 gcctgcaggg cgaccacttt cgcgcccaag cacacgaggc cgcccatgtc gggctggtcg
      361 atcccgctgc agtcgacgtt gttcaagctg agctcacacg aactcacgac cacagcgacc
      421 acgagcaggg tcgccgtcaa caccaccatc ttgcggttcc gcgtcatcgt gccctccccg
      481 gcgtcgtcgc tacccgcaac ctatcgggtc ggctcgtcgc gagcggcgga acgtgcacct
      541 accgacgggt tggtacccca accggcaccg cttcgaaccg tcggcccgga gggtcgacat
      601 cctccggcgc caccgacggt ttggtacccc caacgggatt cgaacccgtg ctgtcgccgt
      661 gaaagggcga tgtcctaggc cgctagacga tgggggcagg tgcacggccg cgcacgaccg
      721 aatgtagcag cgatgcccac gctccctttc gaccgtcgcc ggtacactcg gcccggcagt
      781 cgcccggagc tacggagcgc ggaccaagga tgtcgatgta ccccgatccg tacggtgcct
      841 actggccgcc tcccccgccc agcggaccct ccgtgcgcac ccgggtgggg ctcgcgatgg
      901 cggcagcgct cgcgctcgtg ggggcgagcg cagtcgccgg cgccgtggta ttcgacggcg
      961 gcccgatcta ccccgaacgg tgggaccccc gggtcgccga gatcgcccga ttcgtcgagg
     1021 aggagcgtgg ccacacgtac cggcatccgg tgcgcgtcta cttcctcgct cccgaccgct
     1081 accgcgagat cgccggcggc ggtcccgacg aagctgctcc gaccgacgag gaacgcgagt
     1141 actccgagaa caccgtggcc gagtaccggg ccctcggtct gatggagggc gatcccgacc
     1201 ttctcgccgc caacgagacg atgcaggact cgggcacact cgccttctac tcacccgagg
     1261 acgatgtcgt gaacgtgcgg gggaccgaca tcacgcccgc gatgcgcgtc accctcgccc
     1321 acgagctgac ccacgcgctg caggaccagt acttcgatct ctcgcccgtc ggcgacgcgg
     1381 ccacggccga cgagtccggt gcggcgcgtg ccatcgtcga gggcgacgcc gtctcggtcg
     1441 aggacgccta cgtggcggcg atgtcggacg aggaccgcgc cgactacgag cgggagtcgt
     1501 cgagcgacag cgacgcagca gacaccgacc tcggcgacgt ccccgcagtg ctgcaggtgc
     1561 tgttcgggtc gtactacgcg gtcggaaacg ccttcgtgga gttctggcag gcggatccga
     1621 gcggggtcgt cgaccgcgag aatatcgacg cggtgctgcg gcggttaccg gagggttccc
     1681 atcaactgtt cgaaccggcc cggtatctgg acttcaccga gccccaggtc gtggagccac
     1741 ccgtcgacga gtcgtcgagc gacgagttcg agcggagctc gcacggggtc gacctgttgt
     1801 acgtcatgct ggccgagcgc atcgacccgt tgacggcgct gcgcgccacc gacgggtggt
     1861 ggggcgatgc ctacgtcgca gcggtcggcg acaacgacaa gatgtgtgtg tcctcgcggg
     1921 tcgccttcgg ggccgaggag gacgtgaccg agttccgcga tgcgctcaac gagtggtcga
     1981 cgacgatgcc ggccgatgcc gaggtggcga tccgcaacga cgacgaaccg ctgcaggtca
     2041 cgttcacgac ctgtgatccg gggccgctcg cggagatgaa cctgaccgac caggcggggc
     2101 cggcgctcgc gttcccggtg acccggttgc gcgctgcagc cgacgccgtg tccaacggac
     2161 ggacccgcga gtcagcgctc tgctacggcc aggcgctgat cgatcggctc acgctcactg
     2221 acctgcagtc cgacgaacgc accccggcga tcacggcagc gctcgaggaa gccgcggcgg
     2281 cctgcccctg agcgtcgggc cgagcgggtc ggccgtggtc agatcacagc tcgacctgga
     2341 cgcccagttc gatggtgcgg tcgggtggga gcttgaagaa gacgtcggcc gtcgtcgcgt
     2401 tgcgcagcat cgtgacgaag aggcgctcac gccacttggc catccccggt cggtcggtga
     2461 cccggagggt ctcgcgtccg agcacgaacg tcgccgccga gaagtcgagg cccgtgagcc
     2521 gagcgccgtc ggtggcgagc agcaggggga tgtcgaggtc gtcggaaaag ccggcgtggt
     2581 gcacgagtcg gtggacaccg ttgccgaggt cgtcgtaggt gaggcgttcg tccccgaaca
     2641 cgtgcggtcg gttctcgacc ttcacggcga gcacgaccac cgctgccggg agcaccgacg
     2701 cgtgacggag gtgggacgac agcgcctgcg gcacgatgtc gggattggaa ccgaggtaca
     2761 cgccgacacc ggggccgcgg aggatcggcg tgtcgccgag gttctcgatg aagtcgagca
     2821 gctgcaggtt gcgatgctcg attcgctcgg cggtgatctt gcggccggcg aaccacgtcg
     2881 tcaggacgat gaggaacccg ccgccgatca cgagcggcac ccagccgccg tcggggatct
     2941 tcgggaggtt ggcgccgagg aacccgaggt ccatcaccat gaagagggtg cagagcggca
     3001 acgtcttggc ggtactccag ccgaagcggg tccgggcgac gcggtagaag aggatcgtgg
     3061 tgatcgccat ggtggcggtc accgcgaggc cgtacgccgc ggcgaggttc gacgacgtct
     3121 tgaacccgag caccaggccg acacaggcgg cgagcaggag ccagttgatg gcgggaacgt
     3181 agatctggcc ctcttcgctc tccgacgtgt gggtgatctt cacccgtggc aggtagccga
     3241 gttgcacggc ctgcctggtg agcgagaacg ctcccgagat cagggcctgc gaggcgatga
     3301 ccgtcgcgca ggtggcgaga atgacgaggg gccacagcgc ccaccgcggc gccatctcgt
     3361 agaaggggtt ctcgaaccgc ttgcccgggt tcgagagcat catggcgccc tggccgaagt
     3421 agttgagcag cagcgccggc aggaccagtc cgtaccaggc gaacttgatg gggttccggc
     3481 cgaagtggcc catgtcggcg tagagggctt cgccgccggt cacgaccagg aacaccgagc
     3541 ccagcacgag gaatccgagg atgccgttgt gcaccatgaa ggccacggcg aagtggggag
     3601 acagtgcccg gaggatgctg aggtcatccg cgatgtggct gaccccgagc accccgagta
     3661 cgaggaacca caccaccatg atcgggccga acaccttgcc gacggctgcg gtgccccgct
     3721 tctgcacggc gaacagggcc acgaggatga cgatggcgat cgggatgatg tagtcgtcga
     3781 gttcggtcgt cacgaccttg acaccctcga ccgccgacag caccgagatc gccggcgtga
     3841 tcatgccgtc gccgtagagc agtgacgccc cgaagaggcc gagcagcacg agccaccgcg
     3901 tggagccgcc cagcagcgag cccgacggtc gcacgagcgc cgtgagcgcc aggattcctc
     3961 cttcgccccc gttgtcggct cgcatcacga agagcaggta cttgatcgag atgatgatga
     4021 cgagggccca gaacacgagg gagagcagtc ccagcacgtt cgcctcgtcg acgacgagct
     4081 gccgaccatg gtgttcgtgg aacgactcac gcagcgcgta gagcgggctg gtgccgatgt
     4141 cgccgaagac gacgccgagg gcgccgagcg tgagcgcctt catcgacgaa ctgtggtgac
     4201 cgggggagtc cgacatatgt ctgcaatatc ctagagcgat cgggggaggg cgccgtgaac
     4261 tcggggtcga cggccggtca caggcccccg ctgagcgctt cgacggtgcg gcccaggagg
     4321 tgcgtcaggc tctcgtagac gatgcggtcg gtgcgcgtgg cgccgtcggg gtcggcgggg
     4381 aacccgctct cgtcgacgtc gagttgtgtg ccgagcacga ggcgggcgtc gttgaggcat
     4441 cgcatgaagg cgttgagctc gtcctcggtg cacgaacgtc gagtcatgag ctcctcagtg
     4501 acctgcagcg cagcgaggcg cttgtcgatg agttcgccgc gcatcaggac gtcccatccg
     4561 gcattgcgct cggcatcgga tccgtacgcc gacgggaaca ggcgcgagat cgccgggtcg
     4621 tcgctgctca tcaacgaacg cagctcggcg atcgcctcgc ggaccagcgc tcggtcgacc
     4681 aagctccacc gcaacgtgaa cgtcccgtcg tcgcggggtc ggatgcgggt gaggaacatg
     4741 tcctcgcagt ctgctgccag tttgccgatc gcagccaccc aggccagctc ccgttccagg
     4801 tccgccccgt cgggatcgtc ggcctggagg ctgagcgacc agatggtggt caggagttcc
     4861 gacgactggg tgaggtcgac gagctcgagg tagtcgagcg tcgccggtga atgccgaacg
     4921 atgtcgagac acgtcgagga ggcgtcgtcg atcagtcggg cgatctcggt gcgctcgagc
     4981 agtcgccgcc cggtggggga gtcgacctcg acggtgagct cggtcgagaa cgcatccgct
     5041 gcctgctcga ggcgcgcgac catcaacgcg aggcgctccg ctgggacggc gatcgtgaac
     5101 tcgtcgtcct cgccgagcac gatccacggc ggctcgtcat cgagatcgcg ctcgtcggcg
     5161 ccggaccagg cgtcgggcat gccgctcaat cgtcctgctg catggtggcc cagaggccgt
     5221 gctcgtggag tcggaagacg tcgcgttcgg ccgattcccg cgtgccgctg ctcacggcgg
     5281 cccgtccgtt gaggtgcacc tccaacatga gcgcgtgggc cttggactcg gagtacccga
     5341 acagctttcg cagcacgaag gtgacgtagg gcatgagatt gatggggtcg ttccagacga
     5401 gcacgaccca cggtcgctcg acgtcgaccg tctcgtcggg ctcgagcacc ccgacgggac
     5461 tggacggctc gaaggtgggc tcgctcatcg ggtcgccgtc accgaccgac gggggcgccg
     5521 gcccgatcgc ggcggggtgt ctcgtgcacc gcgctcggcg acggtgcggc gtccgccgtg
     5581 acgaaaccga cgcggagggc catgacccac acgacgatgg cgccggcgac atggaacccg
     5641 accatgagcg ccggaacgtc gctgaagtac tgcacgtagc cgagcgcacc ctgcgcgacg
     5701 acgaccacca ggaggcgggt gcacgtctgg aaggccgccg gcgtgcggtc gcggagccgg
     5761 agcgcggtca cgatcgtcgt gaggcagagc agccaaacga ggatcgagtg gatccgcacg
     5821 accgagctga cgtcgaaggg caaccgctcg accacgtgct cgacgccggc ggaatcgggg
     5881 agcgcgccgg ggtgcttacc ggccgaggtc accatcgttc cgctgacgag cacggccgtg
     5941 ccgagtccga cgaggacctt cgcttcggtg gtccagcggc gcggcgccgg cggcgccacg
     6001 gtgccgtcga ggacctcggg gcggtcgaga tcctcgtcgg cggcccggtg catgaggacc
     6061 acggcggccc agaccaggac catggacacg aggaagtgga cggcgacgac ggagtacttc
     6121 agctcgctca gcgtcacgaa cgcaccgatg atgatctggg cgacgacccc ggccacgagg
     6181 aaccacgacc atcgggtgag gtcgcggcgt cggggacgtc ggcgcatcga accgagcacc
     6241 gcgagggcta ctgacgccga caccagaccg gtgatgagtc ggttgacgaa ctcgaccatg
     6301 gggtggaact cgaggggggc aaccggtttg ccctgctcgc acagtggcca gtcggtgcag
     6361 ccgagccccg agcccgtcag ccgcaccgcg gcgccggtca cgatgatggt cgcgagcccg
     6421 gccagcgcga tctgggtgat gcggacgtac tggcgtggag tcggccggaa tcggtcagcg
     6481 agcagcacgt cgccaccgta cgccccgccg gcgccgacgg gccagcgggc cccggccgca
     6541 gcctccggcc cggtgtggcg tgagggtgac cgagatgcgt cgacggtcgg cggggtcgtc
     6601 tccgcgtcgc attggccatg cggcgggcaa cggacctacc ctctggccgg tgtcatcttt
     6661 gatcgcgacc cccgtatcca cagctccggt gacgcacggt gtgatggcga aagtcggcgg
     6721 ctacgtcgct ctcaccaagc cccgcatcat cgaactcctg ttggtcagca ccgttccgac
     6781 gatgatcgtc gccgaacgtg ggatcccctc gctctggctg atgttcgcga ccgtcgtcgg
     6841 cggaacgctg tcggccggtg gtgccaacgc catcaacatg tacgtcgacc gcgacatcga
     6901 tgccttgatg gaccgcacca agggtcgtcc gctggtcacc ggggtgatga gcccccgcgc
     6961 cgcgctggtg ttcgccgtgg ggatcgagat cgtctcgttc gccttcctct gggcgttcgt
     7021 gaacctgctg tcggcggtcc tcgccgtgtc ggcgtgtctc ttctacgtct tcgtgtacac
     7081 gctctggctc aagcggacct ccgaacgcaa catcgtcatc ggcggcgccg ccggcgctgc
     7141 cccggtcctg atcgggtggt cagcggtcac caacagcctg tcgtgggccc ctgtggtgct
     7201 gttcgccgtg atgttctact ggacaccgcc ccacttctgg gccctggcca tcaagtacaa
     7261 ggacgactac agcaaggccg acgtgccgat gctgccgtcg gtcgccgcgt acgagacggt
     7321 gacccgcaag atcatcgcct acacggtggt ggtcgtggcc ctgacgttcg tgttcggccc
     7381 cgtggccgat ctcggcgccg tctacctggt gtccgccggc gtgctcggcg ccgtgttcct
     7441 cgccctggcc gtcaaggtgc accgcaccga atcggcggcc gctgcgatga agctgttcac
     7501 gtactccatc acctacatca cgctgctctt cggggcgatg gcggtcgatg tactcgtgaa
     7561 gcacggtgcc tgaggtggcg atcaggaacc gagttgacgc cggtaagatg accacccggt
     7621 tttccccagc agatggatgc cgaagtagtg tcctctgccg cagtaccgtc cgggcagcgc
     7681 gccgccgcgc cgcgacgtcc gtccgttcga acgatcacga gcctcgaggc aacacatggc
     7741 actgaccgag acccgaccgc aagctgctgg cgccgcgagc cgtcccgacg gcggtgcccc
     7801 cgagcgcacg caggtgacgg ccgtcgagaa ggtgctcggc accggcgacc acaagacgat
     7861 cggccgcatc ttcatcggtg cgtcgctcct gttcgtcatg tccgacctgg tgatggcggc
     7921 cctcgtcaac ctgcaggcgt ccgccggtgg tgacttcctc gaggccacca tcgccaaccg
     7981 cctgtcgttg aaccacccgc tcggcctgct cctctgcggc gcgttgccgc tgatgctcgg
     8041 cctggcgatc tacctggtgc cgctgcaggt gggctccccc accatcgcct tcccccgggc
     8101 cgccgcggcg tcgttgtggg gctggctcct cggcacggtg ctgttcgtcg tggcgctcct
     8161 catc
//
LOCUS       DAOSOA010000128         8151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8785_length_8151_cov_6.431818, whole genome shotgun sequence.
ACCESSION   DAOSOA010000128 DAOSOA010000000
VERSION     DAOSOA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8785_length_8151_cov_6.431818"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKY13_09325"
     CDS             complement(<1..540)
                     /locus_tag="PKY13_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80175.1"
                     /translation="MTVTDPPRPPVSGSGSGSASAVGEVDPHDPIDRSGRWKVLARPF
                     SIGTLAAVGSTAAGSLVGAIASVDSPVAAVAGTTIDNVPGPVRRWAISTFGTSDKLVL
                     GIGIVVVLALIATFVASAAARRRWVVTAAIVALMLIGFLALDDVTPGAVVATIASALA
                     AWVPFGVLLRFAAPPRRVPV"
     gene            complement(545..3424)
                     /gene="gcvP"
                     /locus_tag="PKY13_09330"
     CDS             complement(545..3424)
                     /gene="gcvP"
                     /locus_tag="PKY13_09330"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOT80176.1"
                     /translation="MPSTDQPSIIELLSAGREERFADRHLGAAEADLAVMVAAVGYGS
                     VDELIDHVVPESIRNRDLLDLPPALTEVEALARIRSYADRNRPLVSMIGRGYHDTTTP
                     PVILRNILENPAWYTAYTPYQPEISQGRLEAIINFQTMVADLCGLDIANASMLDEGTA
                     AAEAMTMARRATKTKAEAFFVDIDCHPQTIAVVETRAQPLGIKVLIGDPATDLDPASV
                     FGALLAYPGSSGAIVDPRRVIESLHEHGALAVVVSDLLALCLLEAPGTLGADIAVGSA
                     QRFGVPMGFGGPHAGFLASTDAHKRSMPGRLVGYSLDAGGRPGLRLALQTREQHIRRE
                     KATSNICTAQVLLAVMASMYAVYHGPEGLRRIAARTHAMASRLAAGLAAAGIPTTNDV
                     WFDTLTVEVPGRAAAVIAAALERGINLRPVDADHVGVSCDETTTVEVIESVLAAFGAG
                     GMPADETVATGLPTALARTTDFCAHPVFHSHRSETEMMRYLRRLADKDIALDRAMIPL
                     GSCTMKLSAATEMIPVTWPEFGRLHPFAPLDQAQGYLDLFADLEAWLATITGYDAVSL
                     QPNAGSQGEYAGLMAIRAYHQANGDLGRTVCLIPSSAHGTNAASAVMVGMKVVVVACD
                     DDGNVDVDDLRTKIEAHRAELAALMVTYPSTHGVFEEAIGEICGLIHDAGGQVYLDGA
                     NMNALVGLAQPGKFGSDVSHLNLHKTFCIPHGGGGPGVGPIGVGAHLAPYLPSTGGAS
                     EGGSVGPVSAAPWGSASILPISWSYIAMMGPDGLRSATTSAIVAANYIAKRLDPHYPV
                     LYSGRDGLVAHECIVDIRPIERECGVTGEDIAKRLIDYGFHAPTMSFPVAGTLMIEPT
                     ESESLAEIDRFCDAMIQIRAEIERVASGEWELAESPLRNAPHPAEDLLAEPWTRPYSR
                     ELAAFPVESLRTNKYWVPVSRVDNGYGDRNLVCSCPPVEAFAAPD"
     regulatory      complement(3494..3587)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3775..4689
                     /locus_tag="PKY13_09335"
     CDS             3775..4689
                     /locus_tag="PKY13_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOT80177.1"
                     /translation="MDIEAHLLALAERQHSLATRRQYAELGAGPKATRHLFEGNPRWE
                     PLTDEVIRLRGSRRTDAQAAMAAVLDAGPGAKLSHLSGGNQWGLSCSLRPFHVVTTVY
                     SRRRTTLSRVHQVRTLPDTWTTELDGIPIVRPELLALQIFAVCREARATDLVEHLWAM
                     RLLSGGSLAAFLSQLGERGRNGTAGLRRYLEPRGLNYVPAATNLERRAISILEQAGYP
                     MRRQVDSGGEHWDGRVDLRHETLPLIVEVQSDRFHSALSFRSNDAERRARLEQDGFTV
                     VEVWEDEVWYRPWEVTARVRAKLRQAST"
     gene            complement(4778..5539)
                     /locus_tag="PKY13_09340"
     CDS             complement(4778..5539)
                     /locus_tag="PKY13_09340"
                     /inference="COORDINATES: protein motif:HMM:NF004855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOT80178.1"
                     /translation="MSRPQQFSFPTFESPAEERTHLRQRLAAGFRLFGRFGFDEGVAG
                     HITARDPERSDCFWVNPFGMSFKQIRASDLILVSHEGEVVEGDWPVNQAAFAIHSRVH
                     AARPDVIAAAHSHSKYGRAFSTLGQKLDPITQDSCAFFDDHEVFDDFTGVVNDTAEGD
                     RIAGALGKNKAAILQNHGLLTVGTSVDAAVWWFITMERSCEVQLAAMAAGTPKLIDPE
                     VAAATYRQVGGELAGAFQFHPLWEWITSQEPDLLD"
     gene            complement(5536..6624)
                     /locus_tag="PKY13_09345"
     CDS             complement(5536..6624)
                     /locus_tag="PKY13_09345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT80179.1"
                     /translation="MTAPAPDLPGATVELPGQPDGVSWPTLTWATGAQLTGDADRVSD
                     VLDRAFATNPNPELALSLAFIAVQGGRIVAERYGPGVSAATPLTSWSVAKSITQSALG
                     LALADGVVDLDAVPAAPEWADPSDPRHAISLRHLMAMRSGLEFNEDYVDAGTSHCLEM
                     LFGEASADMAHYAASQQLVAPVDSTFNYASGSTNIVSRAVKQALGLDHAGFDAYLHER
                     LFDPIGMSRATPHYDGAGTFVGSSYVYATARDFARYGYLHLRDGMWDGRRILPEGWVD
                     VARTARSGPDEDGQYYGAFWWVWGDEHGTFAGQGYEGQLVAVVPALDLVLVRLGKSPI
                     AHRPALFQFYRDVISAFADGAAGEGDPR"
     gene            complement(6621..7478)
                     /locus_tag="PKY13_09350"
     CDS             complement(6621..7478)
                     /locus_tag="PKY13_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80180.1"
                     /translation="MQITKTSGADGFVVVDLPDAATATGIVRCARKILQDGAVNLARS
                     LTYSYAAFGIPTSGASAGINADGDGRDEAVAAFVAELAPRVADGSLQLSAGKGVTDAD
                     LEPWAVVADDGADAALVASVVAAAAATVDLAGATVAVEAGAPASEAIAAGFAAAGADA
                     AVLPLAELLAAGRGVVVVGSKAGVLDHENVGALGAAIIVGAAGQAVTARALATARRNG
                     ATVLPDFVSAAGPLLAASGEADIAARVTEVVRSVLDHEEGPYLGACYAAEAFLRTWSS
                     ELPFGRPLA"
     gene            complement(7842..8138)
                     /locus_tag="PKY13_09355"
     CDS             complement(7842..8138)
                     /locus_tag="PKY13_09355"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOT80181.1"
                     /translation="MFRHIVLLTLVPDTTDEQRQFLLDGLATLPPAISEIDSYRYGTD
                     AGLADGNATIGIVADFADQAAYELYRDHPVHRAVIEERILPVLAGRTAMQIVDA"
BASE COUNT         1287 a         2723 c         2976 g         1165 t
ORIGIN      
        1 gacggggact cgccggggtg gggcggcgaa gcgcaggagc acaccgaacg gcacccacgc
       61 ggcgagcgcg gacgcgatcg tcgcaacgac ggcgccgggc gtcacgtcgt cgagcgcgag
      121 gaacccgatg agcatcaggg ccacgatcgc cgccgtcacg acccaccgcc gacgggccgc
      181 ggcagacgcc acgaacgtgg cgatcagggc gagcaccacc acgatcccga tgccgagcac
      241 cagcttgtcc gaggtaccga acgtgctgat cgcccagcgc cgcaccgggc cgggaacgtt
      301 gtcgatcgtg gtgccggcga ctgcggccac cggagagtcg accgaggcga tcgcgccgac
      361 gaggctgccg gccgcggtgg agcccaccgc cgccaatgtg ccgatcgaga acgggcgggc
      421 gagcaccttc caccggcccg atcgatcgat cgggtcatgc ggatcgacct cgccgaccgc
      481 cgacgccgac ccgctaccgg acccactgac aggagggcgg ggcggatcgg tgacggtcat
      541 gggctcagtc tggcgcggcg aatgcctcga ccggtgggca cgagcacacc aagtttcgat
      601 ctccgtaacc attgtcgacc cggctcaccg ggacccagta cttgttcgtg cgcaacgact
      661 cgaccgggaa ggccgcgagc tcacgggagt acgggcgggt ccacggctcg gcgaggaggt
      721 cctcggccgg atggggggca ttgcggagcg gggactcggc gagctcccac tcgccggacg
      781 caacgcgttc gatctcggcg cggatctgga tcatggcgtc gcagaaccgg tcgatctcgg
      841 cgagcgactc cgactcggtc ggctcgatca tcaacgtccc ggcgacgggg aacgacatcg
      901 tcggggcgtg gaacccgtag tcgatcagcc gcttcgcgat gtcctcgccg gtgaccccgc
      961 actcgcgttc gatcggccgg atgtcgacga tgcactcgtg ggcgaccagc ccgtcacgac
     1021 ccgagtagag cacggggtag tgcgggtcga gacgcttggc gatgtagttg gcggcgacga
     1081 tggcactcgt cgtggcgctg cgcagcccgt cgggccccat catggcgatg tacgaccacg
     1141 agatcggcag gatcgatgcc gacccccacg gggccgccga caccggcccg accgagccgc
     1201 cctcgctcgc tccgccggtc gacggcaggt acggggcgag gtgcgccccg acgccgatcg
     1261 gaccgacccc cggtccgccg ccgccatggg ggatgcagaa ggtcttgtgc aggttgaggt
     1321 ggctgacgtc ggagccgaac ttgcccggct gggccaatcc gacgagggcg ttcatgttgg
     1381 cgccgtcgag gtacacctga ccgccggcgt cgtggatgag gccgcagatc tcgccgatcg
     1441 cctcctcgaa cacgccgtgg gtcgacgggt aggtcaccat gagcgccgcg agttcggcgc
     1501 ggtgggcctc gatcttggtg cggaggtcgt cgacgtcgac gttgccgtcg tcgtcgcacg
     1561 ccaccaccac gaccttcatg ccgaccatca cggcgctggc ggcattggtg ccgtgggccg
     1621 acgacgggat gaggcacacc gtgcgcccca ggtcgccgtt ggcctggtgg taggcgcgga
     1681 tcgccatgag accggcgtac tcgccctggc tgccggcgtt gggctgcaac gacacggcgt
     1741 cgtagcccgt gatggtcgcg agccaggcct cgagatcggc gaacaggtcg aggtagccct
     1801 gcgcctggtc gagcggtgcg aaggggtgga gccgaccgaa ctccggccac gtcacgggga
     1861 tcatctcggt ggccgcgctg agcttcatcg tgcagctacc cagcgggatc atggcccggt
     1921 cgagcgcgat gtccttgtcg gcgaggcgcc ggaggtaccg catcatctcg gtctcgctcc
     1981 gatggctgtg gaagaccggg tgggcgcaga agtcggtggt gcgggcaagc gcagtgggca
     2041 gcccggtcgc gacggtctcg tcggccggca tcccgccggc gccgaacgcc gccaggaccg
     2101 actcgatcac ctcgacggtg gtggtctcgt cgcagctgac ccccacgtgg tcggcgtcga
     2161 ccggacgcag gttgatgccc cgctccagtg cggcggcgat caccgcagcg gcccgcccgg
     2221 gcacctcgac ggtgagcgtg tcgaaccaga cgtcgttggt cgtggggatg cccgcagcgg
     2281 cgaggccggc ggcgagccga gacgccatcg cgtgggtgcg agcggcgatg cggcggaggc
     2341 cctcggggcc gtggtacacg gcgtacatcg aggccatgac ggcgagcagc acctgggccg
     2401 tgcagatgtt cgacgtcgcc ttctcacgac ggatgtgctg ctcgcgggtc tgcagtgcga
     2461 ggcgcagacc ggggcgaccg ccggcgtcga gcgagtagcc gacgagccgg cccggcatcg
     2521 accgcttgtg ggcatcggtg gacgccagga accctgcgtg ggggccgccg aaccccatcg
     2581 gcaccccgaa gcgctgggcc gagcccacgg cgatgtcggc accgagcgtg cccggcgcct
     2641 ccagcaggca gagcgccagg aggtcgctga ccacgacggc gagcgcgccg tgctcgtgca
     2701 acgactcgat gacgcgccgg ggatcgacga tcgctcccga cgaccccggg taggcgagca
     2761 gtgcgccgaa caccgacgcc ggatcgagat cggtcgcggg gtcaccgatc aggaccttga
     2821 tgccgagcgg ctgggcgcgg gtctcgacga ccgcgatcgt ctgcgggtgg cagtcgatgt
     2881 cgacgaagaa cgcctcggcc ttcgtcttgg tggcccggcg agccatggtc atggcctcgg
     2941 cggccgccgt gccctcgtcg agcatcgacg cgttggcgat gtcgaggcca cacaggtcgg
     3001 cgaccatcgt ctggaagttg atgatcgctt cgaggcggcc ctggctgatc tcgggctggt
     3061 acggcgtgta cgccgtgtac caggccgggt tctccaagat gttgcgcagg atgaccgggg
     3121 gagtggtggt gtcgtggtag ccgcgtccga tcatggagac gagggggcgg ttgcggtcgg
     3181 cgtaggagcg gatgcgggcg agcgcctcga cctcggtcag ggcgggcggc agatcgagca
     3241 ggtcgcggtt gcggatcgac tccggcacga cgtggtcgat cagctcgtcg accgatccgt
     3301 agccgactgc ggcgaccatt acggcgaggt cggcctcggc cgccccgagg tgacggtcgg
     3361 cgaagcgttc ctcacgaccg gcggacagca actcgatgat cgagggttga tcggtggacg
     3421 gcatcgggcg aactcctgtt ggggttcccc tctgtcaccg ggacccgctg accgggttcg
     3481 attccagagt tgcctacgtc gagcggtccc gggacctgag agattgcggg gcgttgctcc
     3541 ttcggtgccc gacggcggtg ccgccggaac tctcccgctc gatgtcaacg gcgtcggttg
     3601 gtccgcagaa actaccacgc ccgcctccca cctgatcccg gaccagcccg gcgcgccctc
     3661 accggttctg tcctgcgttc tcgcccctcc aggggcggaa cggcaggaca gatcaagtgc
     3721 catgggggaa gcccccgata tcggcggggt gcatgctcca gcgaacgtca ttccatggac
     3781 atcgaggcgc atctcttggc actggccgaa cggcaacact cgctcgcaac tcggcggcag
     3841 tacgcggaac tcggagccgg cccgaaggcc acccgccatc tcttcgaggg aaacccccga
     3901 tgggagcccc tgaccgacga ggtcatccga cttcgaggtt cgcggcgcac cgatgcgcaa
     3961 gcggcgatgg cagcggtgct cgacgccggc ccgggagcga agctgtccca tctgtccggc
     4021 ggaaaccagt ggggactctc ctgcagcctt cgccccttcc atgtcgtcac gacggtatac
     4081 agccgccgaa ggacgacgct ctcgagggtt catcaggtga ggacactgcc cgacacctgg
     4141 acgacggaac tcgacggcat cccgatcgtg aggcccgaac tcctggccct ccagatcttc
     4201 gccgtctgcc gagaggcacg ggcgaccgac ctggtcgagc acctgtgggc gatgcggctg
     4261 ctctcgggag gatcgctcgc tgcctttctg tcccagctgg gcgagcgtgg ccgcaacggc
     4321 acggcggggc ttcgtcgcta cctcgaaccc cgcggcctga actacgtacc ggccgcgacc
     4381 aacctcgagc gacgggcgat ctcgatcctg gagcaggccg gataccccat gcgccgccag
     4441 gtggactcgg gtggcgagca ctgggatggt cgcgtcgacc tccgccacga gacgctgccc
     4501 ctgatcgtcg aggtacagag cgaccgattc cactcggccc tctcgttccg ttcgaacgac
     4561 gcggagcgtc gcgctcggct ggagcaggac ggcttcaccg tcgtcgaggt gtgggaggac
     4621 gaggtctggt accgcccgtg ggaggtcacg gcccgcgtcc gtgcgaagtt gcggcaggcc
     4681 tccacctgat ttgtcctgcg ttctcgcccc tccaggggcg gaacggcagg acagaagcgg
     4741 tgaggtccgc gcagacgggg ttgcggaggg cgagggctca gtcgaggagg tcgggctcct
     4801 gggacgtgat ccactcccac aacgggtgga actggaacgc cccggcgagc tcgccaccga
     4861 cctggcggta ggtggccgcc gcgacctcgg gatcgatcag cttcggggtg ccggcggcca
     4921 tggcagcgag ctgcacctca cacgaacgct ccatcgtgat gaaccaccac acggcggcgt
     4981 cgaccgacgt gccgaccgtg agcagcccgt ggttctggag aatggcggcc ttgttcttgc
     5041 cgagggcccc ggcgatgcgg tcgccctcgg cggtgtcgtt gacgacgccg gtgaagtcgt
     5101 cgaacacctc gtggtcgtcg aagaacgcac aggagtcctg cgtgatcgga tcgagcttct
     5161 gcccgagggt cgagaacgcc cgcccgtact tcgagtgcga gtgggcggcg gcgatgacgt
     5221 cggggcgagc ggcgtgcacc cgcgagtgga tggcgaaggc cgcctggttg accggccagt
     5281 cgccctcgac gacctcaccc tcgtggctca cgaggatcag gtcggaggca cgaatctgct
     5341 tgaagctcat gccgaacggg ttcacccaga agcagtcgga acgctcgggg tcacgggccg
     5401 tgatgtgccc ggcgacgccc tcgtcgaacc cgaatcgccc gaacaaccgg aaaccggcgg
     5461 cgagtcgctg ccgcaggtgg gttcgttcct cggcgggcga ctcgaaggtc gggaacgaga
     5521 actgctgtgg tcgactcatc ggggatcccc ttctccggca gcaccgtcgg cgaaggcgct
     5581 gatcacgtca cgatagaact ggaagagcgc cggccggtgc gcgatcggcg acttgcccaa
     5641 tcgcacgagc accaggtcga gcgccgggac gacggcgacg agctgcccct cgtagccctg
     5701 cccggcgaac gtgccgtgct cgtcgcccca gacccaccag aacgcgccgt agtactggcc
     5761 gtcctcgtcg gggcccgagc gggcggtgcg ggcgacgtcg acccaaccct cgggaaggat
     5821 ccgacggccg tcccacatgc cgtcgcgcag atggaggtag ccgtagcggg cgaagtcccg
     5881 ggccgtcgcg tacacgtagg aggatccgac gaaggtgccg gcgccgtcgt agtgcggggt
     5941 cgccctcgac ataccgatcg ggtcgaacag ccgctcgtgg aggtaggcgt cgaagcctgc
     6001 atggtcgagg cccagcgcct gtttcacggc gcggctgacg atgttggtcg agcccgacgc
     6061 gtagttgaac gtcgagtcga cgggcgccac cagctgctgg ctcgccgcgt agtgggccat
     6121 gtccgccgac gcctcaccga acagcatctc gaggcagtgc gacgtgccgg cgtcgacgta
     6181 gtcctcgttg aactcgaggc ccgatcgcat ggccatcagg tgccggagcg agatggcgtg
     6241 tcggggatcg gaggggtcgg cccattcggg cgcggccgga acggcgtcga ggtcgacgac
     6301 gccgtcggcg agggcgaggc cgagtgcgct ctgcgtgatc gacttggcga ccgaccagga
     6361 cgtgagcggc gtcgcggcgc tgacgccggg gccgtagcgc tcggccacga tgcggccgcc
     6421 ctgcacagcg atgaacgcca ggctgagggc gagttcgggg ttcgggttcg tcgcgaacgc
     6481 ccggtcgagc acgtcggaga cccggtcggc gtcaccggtg agctgcgcgc cggtcgccca
     6541 ggtgagcgtc ggccacgaca cgccgtcggg ctggcccggc aactcgacgg tagcgccggg
     6601 caggtcggga gcgggtgccg tcatgcgagg ggacgcccga aggggagctc ggaggaccag
     6661 gtgcgaagga acgcctcggc cgcgtagcag gcaccgaggt acggaccttc ttcgtggtcg
     6721 aggaccgagc ggaccacctc ggtcacccgc gcggcgatgt cggcctcgcc ggaggcggcg
     6781 agcagcgggc cggcggcgct cacgaagtcg ggcaacaccg tggcaccgtt gcgccgcgcc
     6841 gtggcgaggg cccgggccgt gacggcctgg ccggcggcac cgacgatgat cgctgcgccg
     6901 agggcgccga cgttctcgtg gtcgaggacc ccggccttgg agcccaccac cacaacgccg
     6961 cgaccggcgg cgaggagctc ggcgagcggg agcaccgcgg catcggctcc ggccgctgcg
     7021 aatccggccg caatcgcctc cgatgccggg gcaccggctt cgacagcgac cgtggcaccg
     7081 gcgaggtcga cggtcgcggc cgccgccgcc accaccgacg ccacgagcgc cgcatcggcg
     7141 ccatcgtcgg cgacgaccgc ccacggctcc aggtcggcat cggtcactcc cttgccggcg
     7201 ctgagctgca gcgagccgtc ggccacgcgt ggcgccagct cggcaacgaa ggccgccacg
     7261 gcctcgtcgc gaccgtcgcc atcggcgttg atgccggccg acgcccccga cgtggggatg
     7321 ccgaacgcgg cgtaggagta ggtgagcgaa cgagcgaggt tgacggcgcc gtcctggagg
     7381 atcttgcggg cgcaccggac gatgccagtg gcggtcgctg cgtcggggag gtcgacgacg
     7441 acgaagccgt cggcgcccga ggtcttggtg atctgcacgg ctgcattgtg ccatgtgacc
     7501 gttcggcgag ccgcggccga tccgatctgt ccggaagcgg ttcgcccgac cctgcagttc
     7561 tgtcctgcga accacgcaca cttcaggccg gttcgcagga cagaacgcaa aggttctccc
     7621 actaacacga cgaggggccg catgagcaga ccccccgaac tgctcacacg gcccatcgat
     7681 tggcggcaaa ccggggggcc gaggcctgcc ggatcactgc gactccgagg cattccgcct
     7741 cggaacggca ccatagcaag gcgtggtgac atctgtcacc cagaaactgg gtgcagtcat
     7801 cgcgagcagt cgttacacag tcgcgagcag caccgggagg tctacgcgtc gacgatctgc
     7861 atcgccgtgc gacccgcgag gacagggagg atccgctcct cgatgaccgc acggtgcacc
     7921 gggtggtcgc ggtacagctc gtacgccgcc tgatcggcga agtcggccac gatgccgatg
     7981 gtcgcgttgc cgtcggccag accggcgtcg gtgccgtagc ggtaggagtc gatctcggaa
     8041 atcgccggcg gcagcgtggc gagcccgtcg aggaggaact ggcgttgctc gtcggtggtg
     8101 tcgggcacga gcgtcaggag cacgatgtgg cggaacatgg cccgaagcta c
//
LOCUS       DAOSOA010000129         8019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_8977_length_8019_cov_8.015445, whole genome shotgun sequence.
ACCESSION   DAOSOA010000129 DAOSOA010000000
VERSION     DAOSOA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8977_length_8019_cov_8.015445"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKY13_09360"
     CDS             complement(<1..596)
                     /locus_tag="PKY13_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT80182.1"
                     /translation="MPRGQLRIYLGAAPGVGKTYAMLQEGHRRRERGADVVIGVVETH
                     GRPSTIEQIGDLEVVPRRHLTYRGAELEEMDVDAIIERAPRICLVDELAHTNVPGSRT
                     AKRYEDVELLLDAGIDVISTVNIQHLESLNDVVERITGAAQREIIPDALVRAADQVHL
                     VDQTPEALRRRMAHGNIYAPERAEAALANYFRAGNLGAL"
     gene            complement(631..1254)
                     /gene="kdpC"
                     /locus_tag="PKY13_09365"
     CDS             complement(631..1254)
                     /gene="kdpC"
                     /locus_tag="PKY13_09365"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HOT80183.1"
                     /translation="MNTSKQSQVVGSAGRPFVKQLLPAVLMVALFTVGLGVVYPLAVT
                     GIAQVTMSGRADGSLVERDGAVVGSSLIGQSFTAPEYFHPRPSAAGTDGYDAMASGGS
                     NLGPSSADLIAAVGERVAAYRAENGLADDVPVPADAVTTSGSGLDPHISIANARLQTR
                     RVADERGLSVDRVEELVDANTDGRALGFLGQPGVNVLGLNLALDAAS"
     gene            complement(1254..3347)
                     /gene="kdpB"
                     /locus_tag="PKY13_09370"
     CDS             complement(1254..3347)
                     /gene="kdpB"
                     /locus_tag="PKY13_09370"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HOT80184.1"
                     /translation="MTAVLTEATPEEPPELAGKESAQLFSAEIARSAIVDSLKKMHPR
                     VQAKNPVMFVVLIGSVLTTVLFLKDLGSSTAQENVFSGVVALFLWFTVLFANFAEAVA
                     EGRGKAQADTLRKTRSETTARRRSADGSIETVSSAILDLDDVVVVTAGEIIPSDGEVV
                     EGIATVDESAITGESAPVIRESGGDRSAVTGGTRVLSDEIVVRITARAGETFVDRMIA
                     LVEGSARQKTPNEIALNILLAGLTIIFLLATVTLQFFAVYSGARQSIIVLTALLVCLI
                     PTTIGGLLSSIGIAGMDRLVRRNVLAMSGRAVEAAGDVATLLMDKTGTITFGNRQAAE
                     FRPVPGTSADELAAAALASSLADETPEGRSIVVLAETAHSQTPPELPDAELVPFTAQT
                     RMSGIDDGDMSIRKGASDSVKAWVLGRGGSVPPDLDDLVRGVSELGGTPLVVATGDAS
                     GTRVLGVIYLKDVIKPGIAERFTQLRAMGIKTVMITGDNPITAKYIAAEAGVDDFLAE
                     ATPEDKMELIKAEQAGSRLVAMTGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDL
                     DSDPTKLLDIVEIGKQLLITRGALTTFSIANDVAKYFAIIPAMFAGVLPALDRLNVMH
                     LQSANSAILSAVIFNALIIVILIPLALRGVKFVPSSAAALLRRNLLIYGLGGLVSPFI
                     GIKLIDMGLTAIGVK"
     gene            complement(3360..5084)
                     /gene="kdpA"
                     /locus_tag="PKY13_09375"
     CDS             complement(3360..5084)
                     /gene="kdpA"
                     /locus_tag="PKY13_09375"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HOT80185.1"
                     /translation="MQATDWLQAAALLIVLGVACPLLGRYIAAVFADGDPAERRAPGD
                     RVFGPIERLVYRVAGVDPTREQRWNIYAVSVLAFSTVSILAVYVLQRIQGSLPLNPAD
                     LRGVTPGIAWNTAVSFVTNTNWQAYAGESTMSHLTQMAALAVQNFVSAAVGLAVAVAL
                     IRGLVRQRRGTIGNFWVDLTRGVTRILLPISFVAALVLAGAGVVQNFSGGTTVRTLEG
                     ASQVIPGGPIASQEAIKEVGTNGGGFLNANSIHPFENPNGLTNFLQIVLLLLIPFALA
                     FAFGRMAKDRKQGYVVAATMFVLLFGLAANNVYQETHGNPRLTEVGVSQAATAENPGG
                     NFEGKDVRIGSASCGEFSAATTNTSTGAVNCQHDSMTPLGGGGALFGMMLGEISPGGV
                     GSGLYGMLVFVLMAVFLAGLMVGRTPEYLGKRIQATEIKLVVLYILFVPLVVLGFGAV
                     SVVIGSGLEGQLNSGAHGLSEILYAFTSAGNNNGSAFGGLTVTNDWYTTTLGLCMLIG
                     RFFLIVPALAIAGSLVRKRRVPVTSGTFPTNTPLFAGLLTVTVLIVVGLTYFPMLALG
                     PIVEHLAL"
     gene            complement(5084..5173)
                     /locus_tag="PKY13_09380"
     CDS             complement(5084..5173)
                     /locus_tag="PKY13_09380"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HOT80186.1"
                     /translation="MSGEEIAALVVAVVLLAYLIAAFVVPEKF"
     gene            complement(5170..5349)
                     /locus_tag="PKY13_09385"
     CDS             complement(5170..5349)
                     /locus_tag="PKY13_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80187.1"
                     /translation="MRDVMYVVVTVAFFALCTGLVRACDLLLGPDEDELLDVDDDPGS
                     DTEVDELVAVSGSWS"
     gene            5524..6297
                     /locus_tag="PKY13_09390"
     CDS             5524..6297
                     /locus_tag="PKY13_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOT80188.1"
                     /translation="MDIQQYSSRREAETGTGTDWFVGLGGFGVSLLIAGVLEGPRATI
                     GSTNVALFLAVVVVAAGVLGRRAGGWITALGAALSFNFFHTRPVHTLRIASGRDVITV
                     VLLAALGGLAGELAHRRQTAVRNAEGGRESTRVLHLNAEECRTATSANAAVLSSTAAI
                     TQLLGAESVAFEHGAAGGPGSRRIEHNGTLTGVPLRATPSGYDLGEIPVTIDVTARDR
                     ILGRFLVVPTDGHPVSLDARLGAIVVADQLSLALVGTLA"
     gene            6389..6919
                     /locus_tag="PKY13_09395"
     CDS             6389..6919
                     /locus_tag="PKY13_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80189.1"
                     /translation="MKKLLLACAALTLATTACFVDPPATPPTGGNWATCPTAAVGQVQ
                     VAIVTEGVPNPAHEVVCVVVPDGSSSIQALQARAARLGTEAPRLAFGGSFVCSIDGNP
                     VAPECGELPGDATGWRSWNYSNGGAQWTGSQVGAASHSVHQGDVDGWNFGTWNYSTTF
                     PGNPTHSPSFSVLTGS"
     gene            6958..7785
                     /locus_tag="PKY13_09400"
     CDS             6958..7785
                     /locus_tag="PKY13_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT80190.1"
                     /translation="MAGEWSNLKEVARTLDVHYMTAYRYVRQGRLPATWVDNGWQVHR
                     DDLAEFVASPGRTPDQAPVDWAARFAPHLVNGDEPGAWSVIESALAAGVDARGCYLGV
                     LSAALEQLGEVWATGSDDDHVDQRLATATALRIVHRLGARFTRPGRRKGSIVLGSPIG
                     ERHTLPVAIAADLIRLAGFTVLELGADVSSETFVAAAQRTERLVAVGISLTTAEGLDA
                     ALHVQRRLAEELPDVPVIVGGQAIANAEVAAMVGSAAWAANGADLVDVLDELARAHR"
     gene            complement(7795..>8019)
                     /locus_tag="PKY13_09405"
     CDS             complement(7795..>8019)
                     /locus_tag="PKY13_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80191.1"
                     /translation="PAIFAHAASGLGVDDPSAFAHVGDLRRTDVGGATAAGWTAVRYR
                     GLNDDVDASSPDAAIVVDHHGELLAALGLA"
BASE COUNT         1298 a         2771 c         2718 g         1232 t
ORIGIN      
        1 agtgctccga ggttgccggc gcggaagtag ttcgccaacg ccgcctcggc gcgttcgggt
       61 gcgtagatgt tgccgtgggc catacggcgt cgcagtgcct cgggcgtttg gtcgacgagg
      121 tgcacctggt cggcagcccg aaccagcgcg tcgggaatga tctcgcgttg cgccgccccg
      181 gtgatgcgct cgacgacgtc gttgagcgat tcgaggtgct ggatgttgac cgtcgagatc
      241 acgtcgatgc ccgcgtcgag gagcagctcg acgtcctcgt agcgcttcgc tgtgcgactc
      301 cccgggacgt tggtgtgggc gagctcgtcg acgagacaga tacgcggcgc ccgctcgatg
      361 atggcgtcga cgtccatctc ctcgagctcc gcaccgcgat aggtcaggtg ccgtcgcggc
      421 accacctcga gatcaccgat ctgctcgatg gtgctcggcc ggccgtgggt ctccaccacc
      481 ccgatgacga cgtcggcgcc gcgctctcgg cgacgatgtc cctcctgcag catggcgtag
      541 gtcttgccca cacccggtgc cgcgccgagg tagatgcgga gctgaccacg aggcacggaa
      601 cgatcctacg agcaggcgcc ggacagacga tcaggaggcc gcgtcgagcg ccaggttcag
      661 gccgagcacg ttgaccccgg gctgaccgag gaacccgagg gcacggccgt cggtgttggc
      721 gtcgacgagt tcctcgacac ggtcgaccga gagaccccgc tcatcggcga cccggcgcgt
      781 ctggagacga gcgttggcga tcgagatgtg cgggtcgaga cccgagcccg acgtggtcac
      841 agcgtcggcc gggacgggga cgtcgtcggc caatccgttc tcggcccgat aggcggcgac
      901 ccgctcaccg accgccgcga tcaggtcggc gctgctcggc ccgaggttcg agccccccga
      961 cgccatggcg tcgtacccgt ccgtaccggc agcggacggc cgcgggtgga agtactccgg
     1021 tgccgtgaac gactggccga tcagcgacga acccaccacg gcgccgtcac gctcgaccag
     1081 cgaaccgtcg gcacgacccg acatcgtcac ctgggcaatg ccggtgaccg caagcgggta
     1141 cacgacgccg agcccgacgg tgaagagcgc caccatcagg actgccggga gcagctgctt
     1201 gacgaatggg cggccggccg agccgaccac ctgggattgc ttggacgtgt tcatcacttc
     1261 actccgatcg cggtgagacc catgtcgatc aacttgatgc cgatgaacgg cgacacgagg
     1321 ccgcccagcc cgtagatcag caggttgcgg cgcagcaggg ccgccgcgct cgagggaacg
     1381 aacttcacac cacgcagcgc cagcgggatc aggatcacga tgatcaacgc attgaagatg
     1441 acggccgaca gaatcgccga gttggcgctc tggaggtgca tgacgttcag tcgatcgagc
     1501 gccggcagca ccccggcaaa catcgccgga atgatcgcga agtacttcgc cacgtcgttc
     1561 gcgatcgaga aggtggtgag cgccccacgg gtgatcagca gctgcttgcc gatctcgacg
     1621 atgtcgagca gcttggtcgg atcgctgtcg aggtcgacca tgttgccggc ctccttggcg
     1681 gcctgcgtcc cggtgttcat cgcgacaccg acgtcggcct gcgccagggc gggcgcgtcg
     1741 ttggtgccgt ctccggtcat ggccacgagg cgggaaccgg cctgctcggc tttgatcagc
     1801 tccatcttgt cctcgggcgt cgcctcggcg aggaagtcgt cgacacccgc ctccgccgcg
     1861 atgtacttcg cggtgatcgg gttgtcaccg gtgatcatca ccgtcttgat gcccatggcc
     1921 cggagttggg tgaaccgttc ggcgataccg ggcttgatga cgtccttgag gtagatgacg
     1981 cccaggacgc gagtcccgga cgcgtcgccg gtggcgacga ccagcggggt gccacccagc
     2041 tcggacacac cccggacgag gtcgtcgagg tccggtggca ccgagccgcc gcgtcccaac
     2101 acccatgcct tcaccgagtc ggacgcaccc ttgcggatgg acatgtcccc gtcgtcgatc
     2161 cccgacatcc gcgtctgcgc ggtgaacggg accaactcgg cgtcgggcag ctccggtggc
     2221 gtctgtgagt gcgccgtctc ggcgagcacc acgatcgagc gaccttccgg agtctcatcg
     2281 gcgaggctcg acgccaaggc cgccgcggcc aactcgtcgg ccgacgtgcc aggcaccggg
     2341 cggaactcgg cggcctggcg gttgccgaac gtgatcgtgc cggtcttgtc catcagcagc
     2401 gtcgcgacgt cgcccgccgc ctcgacggca cggcccgaca tcgcgagcac gttgcggcgc
     2461 accaggcggt ccatgccggc gatgccaatg gaactcaaga ggccgccgat ggtcgtgggg
     2521 atcaggcaca cgagcagggc ggtcaacacg atgatcgact ggcgagcacc gctgtagacg
     2581 gcgaagaact ggagggtgac cgtcgccaac aggaagatga tcgtcagacc ggccaggagg
     2641 atgttcagag cgatctcgtt gggcgtcttc tgcctcgcgg acccctcgac cagggcgatc
     2701 atccggtcga cgaacgtctc gccggcgcgg gcggtgattc gtacgacgat ctcgtcggac
     2761 aacacccgag tgccaccggt gaccgccgag cgatcaccgc cggactcccg gatgacgggt
     2821 gccgactcgc cggtgatggc cgattcgtcg accgtcgcga tgccctccac cacctcgccg
     2881 tccgacggga tgatctcgcc agcggtgacc accaccacgt cgtcgaggtc gagaatcgcc
     2941 gaactcacgg tctcgatcga gccgtcggcg ctgcggcgtc gggccgtcgt ctcgctccgg
     3001 gtcttgcgga gcgtgtcggc ctgggccttg ccccgtccct cggcgacggc ctcggcgaag
     3061 ttggcgaaca acacggtgaa ccagaggaag agcgccacga cgcccgagaa cacgttctcc
     3121 tgcgcggtgc tcgacccgag gtccttcagg aagaggaccg tcgtgagcac actgccgatc
     3181 aggacgacga acatgacggg gttcttggcc tgcacccgcg ggtgcatctt cttgaggctg
     3241 tcgacgatcg ccgagcgggc gatctcggcg ctgaacagct gcgccgattc cttgccggcc
     3301 agctccgggg gttcttctgg ggttgcttcg gtgagtactg cagtcatcgg ttctgactcc
     3361 tagagcgcga ggtgttcgac gatggggccg agcgccagca tcgggaagta ggtcaggccg
     3421 accacgatca acaccgtgac cgtgaggagc ccggcgaaca gcggggtgtt ggtggggaag
     3481 gtgccgctcg tgacggggac acggcgcttg cgcaccaacg aaccggcaat ggcgagcgcc
     3541 ggaacgatca ggaagaagcg tccgatcaac atgcagagac cgagggtggt cgtgtaccag
     3601 tcgttcgtga ccgtgagccc accgaacgcc gacccgttgt tgttgcccgc cgaggtgaag
     3661 gcgtagagga tctcggacag cccgtgagca cccgagttga gttggccctc gagtccggag
     3721 ccgatgacca cggacacggc tccgaacccg aggacgacca gggggacgaa caggatgtac
     3781 agcaccacca gcttgatctc ggtggcctgg atccgtttgc ccagatactc cggcgttcgg
     3841 cccaccatga ggcccgccag gaacacggcc atgaggacga acaccaacat gccgtagagg
     3901 cccgacccga cgccgccggg gctgatctcg ccgagcatca tcccgaagag ggcaccgcca
     3961 cccccgaggg gggtcatcga gtcgtgttgg cagttgacgg cgccggtcga cgtgttggtg
     4021 gttgccgcgg agaactcacc acagctggcc gatccgattc ggacgtcctt gccctcgaag
     4081 ttgcctccgg ggttctccgc cgtcgcagcc tgcgacacgc cgacctcggt gagccggggg
     4141 ttgccgtgcg tctcctggta gacgttgttg gccgcgagcc cgaagagcag cacgaacatc
     4201 gtggcggcca ccacgtaccc ctgcttgcgg tccttggcca tgcgaccgaa ggcgaacgcc
     4261 agggcgaacg ggatgagcag gagcagaacg atctgcagga agttcgtcag cccgttcggg
     4321 ttctcgaacg gatggatcga gttggcgttg aggaagccac cgccgttcgt cccgacctcc
     4381 ttgatggcct cctggctggc gatcggtccg ccggggatca cctggctcgc gccctcgagg
     4441 gttcgaaccg tcgtgccgcc ggagaagttc tggaccaccc cggccccggc gagcacgagc
     4501 gctgcaacga agctgatggg caggaggatc cgggtcaccc cgcgggtgag atcgacccag
     4561 aagttgccga tggtcccgcg tcgttgacgg accagcccac ggatgagggc gaccgccacg
     4621 gccaggccga ccgcggccga cacgaagttc tggacggcga gcgccgccat ctgggtgagg
     4681 tgggacatcg tcgattcccc ggcgtacgcc tgccagttcg tgttcgtcac gaagctgacg
     4741 gcggtgttcc aggcgatgcc cggtgtgact ccgcgcaggt cggcgggatt gagcgggagc
     4801 gaaccctgga tccgctggag gacgtacacc gcgaggatcg acacggtgct gaacgccagc
     4861 acggacacgg cgtagatgtt ccagcgctgt tcacgggtcg ggtcgacgcc ggccacgcgg
     4921 tacacgagcc gttcgatcgg accgaagacc cggtctccgg gcgctcgacg ctcggccggg
     4981 tcgccgtcag cgaacacggc ggcgatgtaa cgacccagca gtgggcaggc cacccccaga
     5041 acgatcagga gggctgccgc ttgcagccaa tcggtcgcct gcatcagaac ttctccggga
     5101 ccacgaacgc ggcgatcagg tacgcgagca gcacaacggc cacgaccagc gcagcgatct
     5161 cctcgccgct catgaccacg accccgacac ggccacgagc tcgtcgacct cggtgtccga
     5221 tcccgggtcg tcatcgacgt ccaacagctc gtcctcgtcg ggtccgagca acagatcgca
     5281 cgcccggacc aggccggtgc acagggcgaa gaaggccacg gtcaccacca cgtacatcac
     5341 gtctcgcatc tcacacactc ctcatctcga tgccgacggt cggggtccga cgaccggctc
     5401 gattcgaggt ctacgcctcg gtccgaccgt cgagaagagc ctttgcgcgc tcttgacgcg
     5461 gtcgtcgtga accttgacgc gcccttgatt ccgcccggct ccgacccggc gtagaacgag
     5521 ggcatggaca ttcagcagta ctcatcccgc cgcgaggccg agaccggaac cggaaccgac
     5581 tggttcgtcg ggctcggcgg attcggcgtg tcactcctca tcgccggggt gctcgagggc
     5641 ccgcgggcga cgatcggttc gaccaacgtc gcgctgttcc tcgccgtcgt ggtcgtcgct
     5701 gccggtgtgc tcggccgccg ggccggaggc tggatcaccg cgctcggcgc cgcgttgtcg
     5761 ttcaacttct tccacacccg gccggtccac acgctgcgga tcgcgtccgg tcgcgacgtg
     5821 atcaccgtcg tcctgctcgc cgcgctcggc gggttggccg gagaactggc gcaccgccga
     5881 cagaccgcgg tccggaacgc cgagggaggt cgggagagca cccgggtcct gcacctcaat
     5941 gccgaggaat gccgcacggc gacgtccgcc aacgccgcgg tgctgagttc gacagccgcc
     6001 atcacccagt tgctcggcgc cgagtccgtc gcgttcgagc acggcgccgc cggcgggccg
     6061 ggatcacggc ggatcgagca caacggcaca ctcaccggcg tcccgctgcg ggcgactcca
     6121 tccggatacg acctcggcga gataccggtg accatcgacg tcaccgcccg cgaccggatc
     6181 ctggggcgat tcctcgtcgt cccgaccgac ggtcacccgg tctcgctcga cgcccggctg
     6241 ggcgccatcg tcgtggccga ccagctgtca ctcgcgctcg tcggcacgtt ggcctgaggt
     6301 gccctcgatt tagcactaag cgccaggagc tggccttcgg acggggcgat tcgtagggtc
     6361 gatcatccac tgttctggag ggaaccgaat gaagaagctc ctgttggcct gcgctgcgct
     6421 gaccctggca accaccgcat gtttcgtcga cccgcccgcg acgccaccca cgggcggcaa
     6481 ctgggcgacg tgccccaccg ccgccgtcgg ccaggtgcag gtcgccatcg tgaccgaggg
     6541 ggtgccgaac ccggcgcacg aggtcgtgtg cgtcgtcgtg cccgacggat cgagcagcat
     6601 ccaagcgctc caggcacgcg cggcccgcct cggcaccgaa gcgccccgcc tggcgttcgg
     6661 cggctcgttc gtctgctcga tcgacggcaa ccccgtcgct cccgagtgcg gtgagcttcc
     6721 cggcgacgcg accggctggc gttcgtggaa ctactcgaac ggcggcgcgc agtggaccgg
     6781 ttcgcaggtc ggcgccgcat cgcacagcgt ccaccagggc gacgtcgacg gctggaactt
     6841 cggcacctgg aactactcga ccacgttccc gggcaacccc acccactccc ccagcttctc
     6901 ggtcctgacc gggagctgag gtcgacgctc cgctcgcccg ccacgtagcg ttgtcgcatg
     6961 gcgggcgagt ggagcaatct caaagaggtc gccaggacgc tcgacgtcca ctacatgacc
     7021 gcctaccggt acgtgcgcca gggccgactg cccgccacgt gggtcgacaa cggttggcag
     7081 gtccaccgcg acgacctcgc cgagttcgtc gcctcgcccg gacggacgcc cgaccaggcg
     7141 ccggtcgact gggcggctcg tttcgccccg cacctggtca acggcgacga gcccggcgcc
     7201 tggtcggtga tcgaatcggc gcttgccgcc ggcgtcgacg ctcgggggtg ctacctgggc
     7261 gtgctctccg ctgcgctcga gcaactgggc gaggtctggg cgaccggcag cgacgacgac
     7321 cacgtcgacc agcggctggc cacggccacc gcgctgcgga tcgtgcaccg gttgggcgcc
     7381 cggttcaccc gccccggtcg tcgcaagggt tcgatcgtgc tgggctcacc catcggtgag
     7441 cgccacaccc tgccggtggc gatcgccgcc gacctgatcc gactcgccgg tttcacggtg
     7501 ctcgagctcg gggccgatgt ctcttccgag acgttcgttg ccgccgccca gcgcaccgaa
     7561 cggctcgtcg ccgtcggcat cagcctgacg accgccgaag gcctcgacgc cgcgctccac
     7621 gtgcagcgtc gcctcgccga ggagctgccc gacgtccccg tcatcgtggg cggccaggcc
     7681 atcgccaacg ccgaggtcgc agccatggtc ggctcggccg cctgggcggc gaatggcgcc
     7741 gacctcgtcg atgtcctcga cgagctggcc cgcgcccacc gctgacgatc cggatcaggc
     7801 gaggccgagc gcagcgagca actcgccgtg gtggtcgacc acgatcgcag cgtcgggtga
     7861 cgacgcgtcg acgtcgtcgt tgagcccccg gtaccgcacc gccgtccagc cggctgccgt
     7921 ggctccaccc acgtcggtcc gacgcagatc gcccacgtgg gcgaacgcgc tggggtcgtc
     7981 gacaccgagt ccactcgcgg cgtgggcgaa gatcgccgg
//
LOCUS       DAOSOA010000130         7962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9070_length_7962_cov_5.252435, whole genome shotgun sequence.
ACCESSION   DAOSOA010000130 DAOSOA010000000
VERSION     DAOSOA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9070_length_7962_cov_5.252435"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /gene="prcA"
                     /locus_tag="PKY13_09410"
     CDS             complement(<1..304)
                     /gene="prcA"
                     /locus_tag="PKY13_09410"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HOT80192.1"
                     /translation="MSMPFYVAPEQMMKDRADYARKGIARGRSLVAVAYDEGILLCAE
                     NPSRMLRKISEIYDRIGFAGVGKYNEFDQLRIAGVRHADLKGFSFSREDVDARSLAN"
     gene            complement(301..1110)
                     /gene="prcB"
                     /locus_tag="PKY13_09415"
     CDS             complement(301..1110)
                     /gene="prcB"
                     /locus_tag="PKY13_09415"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HOT80193.1"
                     /translation="MLPSFTPADDPGPDFGALVRRVGLNSPAGDASSTDASLITHGTT
                     CIAVRYADGVVMAGDRRATSGNWISHRTIEKVFPADKYSGVAIAGAAGPAIEMVRLFQ
                     LQLEHYEKVEGSHLSLEGKANQLSQMLRNHLPAALSGMAVVPIFAGFDTKRELGRLFQ
                     YDITGGRYEESDYAATGSGSMHAATVTKLGFRPGLTRDEAVDLVIHALWQAADEDSAT
                     GGPDLIRKIFPVVATITAQGFHRLGDDELEERSRALIADIEARRGGGGEAR"
     gene            complement(1116..1310)
                     /locus_tag="PKY13_09420"
     CDS             complement(1116..1310)
                     /locus_tag="PKY13_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HOT80194.1"
                     /translation="MPEREQIKKQAPVRTEEVEVTDVAPSESGSKIKAELDDLLDEID
                     EVLETNAEDFVKSYIQKGGE"
     gene            complement(1378..2910)
                     /gene="dop"
                     /locus_tag="PKY13_09425"
     CDS             complement(1378..2910)
                     /gene="dop"
                     /locus_tag="PKY13_09425"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HOT80195.1"
                     /translation="MAVPIVCGIETEYGIIVRRSGGGEQPESNPMAASSTLVSSYVSD
                     LADQGRGVGRPHRVGWDFEDETPANDARGGPSPYSYAPEVESHLVNAVLTNGSRYYVD
                     HAHPELSTPECRTALQTLLYDRAGEEILLRSMRVAPTFMPPGQEIVVYKNNSDGKGQS
                     YGCHENYMLSRGLPFGKLVTNATAHFVTRQIYCGAGKVGTELPGVRRDAVSYQISQRA
                     DFFEEEVGLETTLKRPIVNTRDEPHADARKYRRFHVIIGDANMSEVATFLKLGTTALW
                     MAVIDDDAVDRTFKFAHPIPALRAISNDLTLSEPVEMIDGTTMTALEIQWDIYRQCAA
                     YAERHGLEAVGTDVGEMVLDRWRAVLEGLESDPMTLAGQIDWIAKYRLLNGYRERHDI
                     GWDDARLRAMDLQYHDLRPEKSLARRVGLETLVDPDDVAAAMSSPPPDTRAWFRGTCL
                     QRFADEIVAANWDSLVFDTGGSSLRRVPMLEPTRGTREHVGELLDSVSSAAELIAKLG
                     AT"
     gene            complement(2965..5502)
                     /locus_tag="PKY13_09430"
     CDS             complement(2965..5502)
                     /locus_tag="PKY13_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80196.1"
                     /translation="MLPRRAVAQFAAVALLFVVGFSALALLVSDPDEAGASTGSARRP
                     APSAESRNPYGEMNLAWTEPGMIGVVGWAKDPDVNRPIEVVVAVDRGLFVRTAGVYNP
                     FLPGSYGEKFRSYLVAIPVTPGPHTMCVAALNVGPGTGFRLLGCKVFGNVESADPQGN
                     LEGATLRPSGDRLQVTGWALDRETSAALAVDLTVDGAPAGRVLASAGRPDLASTFPGL
                     GTGHGFTADVAVAPGRRRVCAIARNYNRGADATIGCTDVDVPATSPSGVIDAVSVAPD
                     AIVVSGWAGDPSPRTPINVVVTDRVVGAVDDAVRTTVAATGSRPDVAATTGLDPRAGY
                     AVALAATVPGVHEICVTAVNVGIGADQFLGCRTVSVVDHRPAFNIEGVQPQGSAVTVS
                     GWAFDPDSSNPVTVRVAVDGAPTVVTANQSRPDVGAAFPGVGQNRGFQSTIGVGGGLH
                     TVCVTFVDQVPGLPGVTGDRAPACGSVIVGPTAIGTTGIAGPTQVVGPDGGPLRDVDR
                     DGGVSVTLRDGSVLWLFGDSTAYNGDGSLRYFVGGTAAWASAAQPTITRDAATPSGAP
                     YVLAQPGEGYPACSASRPNRVMWPVSAVKVPDGGRDRVVAFFANMCIGSVNDYEFKGI
                     SLAQWFYDPASPPIFSPVQFTVINQQLQVTRAFGTASTYDPVSGFAYLYACDRPANPL
                     NVGGYGPCRVARVDPVNIGDPNAYRYFNGTTWTLGESSAQAMDMPQGTSSITRYPAAA
                     FTVTFDPTKGVYVMVYSPWPGFIDRAMVRVSRSPQGPWTAPVEITLPGCSNTIDGQAR
                     NCYAATAQPAFSTGAELGLGFYDQSTPADRNRGRYLVARVPFAVALS"
     gene            complement(5550..7442)
                     /gene="arc"
                     /locus_tag="PKY13_09435"
     CDS             complement(5550..7442)
                     /gene="arc"
                     /locus_tag="PKY13_09435"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HOT80197.1"
                     /translation="MTIASTEVESEDRGVRFQRSEEVVPIDAHDESNGTHDEVGDRPW
                     GDPIGDELRQSSGDEVTDLRDRVAELQGEVSTLLRRIQDAPKRVRTLEERLLETKGQL
                     AQAVAQNEKLSYTLREARDHIAALRDEVDKLTQPPAAYATLLGINDDGTVDIVSSGRK
                     MRVEVSPSIEASELRCGVEVVLNESFTVVMVRSQEIVGEVVTLREVLEDGRRALIVGR
                     ADEERVAELGDALRSQRLRAGDVMLMDTRSSVLLEKLPRPEVDELMLEEVPDIAYSDI
                     GGLDDQIEQITDAVELPFLHQAKFSEYKLPAPKGILLYGPPGCGKTLIAKAVANSLAK
                     KVAEMTGRDQNSYFISIKGPELLNKYVGETERHIREVFQRARDKAADGTPVIIFFDEM
                     ESLFRTRGTGISSDIESTIVPQLLAEIDGVETLRNVIVIGASNREDLIDPAILRPGRL
                     DVKIKINRPDADSAAQIFSQYLTSDLPIDPTEVMTLGGGDVDKAVRVMIETTVEEMYR
                     TDENNQFLEVTYQNGDKEVMYFKDFSSGAMIENVVRRAKKLAIKRELADGAKGIKVDD
                     LLESIHQEFKEHEDLPNTTNPDDWAKISGKKGERIVYIRTLVHREDNRFEGGRAIERV
                     ATGQYL"
     gene            7601..7846
                     /locus_tag="PKY13_09440"
     CDS             7601..7846
                     /locus_tag="PKY13_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOT80198.1"
                     /translation="MGMKVWIDQDLCTGDGLCEEIAPKVFTLLDDGLAYVVDPAGKVY
                     SDPGGPEGLAEVPEGEWEGTIESAEECPGECIFIEME"
BASE COUNT         1201 a         2641 c         2800 g         1320 t
ORIGIN      
        1 ggttggcgag gctgcgggcg tcgacgtcct cgcggctgaa cgagaagccc ttgagatcgg
       61 cgtggcgcac gccggcgatg cggagctggt cgaactcgtt gtacttgccg accccggcga
      121 acccgatgcg gtcgtagatc tcggagatct tgcgcagcat gcggctgggg ttctcggcgc
      181 agagcaggat gccctcgtcg taggcgacgg ccacgagcga gcggccacgg gcgatgccct
      241 tgcgggcgta gtcggcccgg tccttcatca tctgctcagg ggcgacgtag aagggcatgc
      301 tcatcgcgct tcacctcccc cgccccggcg ggcttcgatg tcggcgatca acgcacggct
      361 ccgttcctcg agctcgtcgt cgcccagacg gtggaacccc tgggcggtga tggtggcgac
      421 caccgggaag atcttgcgga tcagatcggg cccaccggtc gccgagtcct cgtcggcggc
      481 ctgccagagc gcgtggatga ccaggtcgac tgcttcgtcg cgggtcaggc ccgggcggaa
      541 gccgagcttg gtgaccgtcg cagcgtgcat cgagccggag ccggtcgccg cgtagtcgga
      601 ttcctcgtag cggccaccgg tgatgtcgta ctggaagagc cgccccagct cccgcttcgt
      661 gtcgaaaccg gcgaagatcg gcacgaccgc catgcccgac agcgctgcgg gcaggtggtt
      721 ccgcagcatc tggctcagct ggttggcctt gccctcgagg ctgaggtggc tgccttcgac
      781 cttctcgtag tgctcgagct gcagctggaa caggcgcacc atctcgatcg ccggacccgc
      841 ggccccggcg atcgcgacac ccgagtactt gtcggcgggg aacaccttct cgatcgtgcg
      901 gtggctgatc cagttgcccg aggtggcgcg acggtcgccc gccatcacca cgccgtcggc
      961 gtagcggacg gcgatgcagg tggtgccgtg cgtgatcagg ctcgcatcgg tggacgaggc
     1021 atcgccggca ggactgttga gcccgacacg ccgcacgagc gccccgaaat ctggccccgg
     1081 atcatcggcc ggggtgaacg acggcagcat gtggcctact cgccgccctt ctggatgtag
     1141 gacttcacga agtcctcggc gttggtctcg agcacctcgt cgatctcgtc gaggaggtcg
     1201 tcgagttcgg ccttgatctt ggaccccgac tcgctcggag cgacgtcggt gacctcgact
     1261 tcctcggtgc ggacaggggc ttgcttcttg atctgttcac gttctggcac tggtctcaac
     1321 ctccggtccc atcaacgtac cgcgcgtgct gatgattcct gtgccatatc gggccactca
     1381 ggtggctccg agcttggcga tcagctcggc ggccgacgac acgctgtcga gcagttcgcc
     1441 gacgtgctcg cgggtgccgc gggtcggctc cagcatcggc acgcggcgca gcgacgagcc
     1501 accggtgtcg aacacgagcg aatcccagtt ggcggcgacg atctcgtcgg cgaagcgttg
     1561 caggcaggtc ccccggaacc aggctcgggt gtcgggcggc ggcgaactca tggcggcggc
     1621 gacgtcgtcg gggtcgacga gggtctcgag cccgacccgg cgggccagcg acttctccgg
     1681 ccggaggtcg tggtactgca ggtccatcgc ccgcagccgg gcatcgtccc agccgatgtc
     1741 gtggcgttcg cggtacccgt tgagcagccg gtacttggcg atccagtcga tctgtcccgc
     1801 gagggtcatc gggtcggatt cgagcccctc gaggacggcc cgccaacggt cgagcaccat
     1861 ctctcccacg tcggtaccca ccgcctcgag accgtgacgc tcggcgtacg cggcgcactg
     1921 ccggtagatg tcccattgga tctcgagcgc cgtcatcgtc gtgccgtcga tcatctcgac
     1981 cggctcggac agtgtgagat cgttcgagat ggcccgcagc gccgggatcg ggtgggcgaa
     2041 cttgaacgtc cgatcgacgg cgtcgtcgtc gatcaccgcc atccacaggg ccgtcgtgcc
     2101 gagcttgagg aaggtggcga cctcactcat gttggcgtcg ccgatgatca cgtggaatcg
     2161 gcggtacttg cgggcatcgg cgtgcggttc gtcgcgggtg ttgacgatcg ggcgcttcag
     2221 tgtggtctcg aggccgacct cctcctcgaa gaagtcggcg cgctgggaga tctggtagct
     2281 gacggcgtcg cggcggacac cgggcagctc ggtaccgacc ttgccggcac cgcagtagat
     2341 ctgacgggtg acgaagtggg cggtcgcgtt ggtgaccagc ttcccgaagg gcaggccccg
     2401 ggagagcatg tagttctcgt ggcagccata gctctggccc ttgccgtcgg agttgttctt
     2461 gtagacgacg atctcctggc ccggcggcat gaacgtgggc gccacccgca tcgagcggag
     2521 gaggatctct tcgccggcgc ggtcgtacag cagcgtctgc agggcggtac ggcattcggg
     2581 cgtcgacagc tcggggtggg cgtggtcgac gtagtaccgg ctgccgttgg tgagtaccgc
     2641 gttcacgagg tgcgactcga cctcgggggc gtagctgtag gggctgggac cacctctggc
     2701 atcgttggcc ggcgtctcgt cctcgaagtc ccatcccacg cggtggggac gtccgacgcc
     2761 acggccctgg tcggcgagat cggacacgta cgacgacacc agcgtcgaac tcgccgccat
     2821 cgggttggac tcgggctgct caccaccacc ggaccggcgc acgatgatgc cgtattcggt
     2881 ttcgattcca cagacgatcg ggacggccac gggcggcagg ctaccgggcg ccgccggtcg
     2941 gactgggccc agggcgtgcc gagatcacga cagcgcgacc gcgaacggga cccgcgcgac
     3001 caggtaccgg ccccggttgc ggtcggctgg cgtcgactga tcgtagaagc cgagaccgag
     3061 ctcggcgccg gtgctgaacg ccggctgggc ggtggccgca taacagttgc gggcctgccc
     3121 gtcgatggtg ttcgaacaac ccggaagggt gatctccacg ggtgccgtcc acggaccttg
     3181 cggggaccgg gagacgcgga ccatggcgcg gtcgatgaag cccggccacg ggctgtacac
     3241 catcacgtac acgcccttgg tcggatcgaa ggtgacggtg aaggcggccg ccggataccg
     3301 cgtgatcgac gacgtcccct gcggcatgtc catcgcctgc gccgagctct cgcccagcgt
     3361 ccacgtggtg ccgttgaagt agcggtatgc gttgggatca ccgatgttca ccgggtcgac
     3421 ccgggcgacc cggcacgggc cgtacccgcc cacgttgagc gggttcgccg ggcggtcgca
     3481 cgcgtagagg taggcgaagc ccgagacagg gtcgtaggtc gacgccgtgc cgaaggcgcg
     3541 ggtcacctgg agctgctggt tgatgacggt gaactggacc ggagagaaga tcggcggcga
     3601 cgccgggtcg tagaaccatt gcgccaacga gatgcccttg aactcgtagt cgttcaccga
     3661 cccgatgcac atgttggcga agaacgcgac gacccggtcg cgcccgccgt cgggcacctt
     3721 gaccgccgag accggccaca tgacccggtt gggacgcgac gcgctgcacg ccgggtagcc
     3781 ctctcccggt tgtgccagga cgtacggcgc ccccgagggg gtcgcagcgt cgcgggtgat
     3841 cgtcggctgg gcggcgctcg cccacgccgc ggtgccgccc acgaagtacc ggagcgaacc
     3901 gtcgccgttg taggccgtgc tgtcgccgaa cagccacagg accgagccgt cgcgcagtgt
     3961 caccgacaca ccgccatcac ggtcgacgtc gcggagcggg ccgccgtcgg gaccgaccac
     4021 ctgggtcggg ccggcgatgc cggtggtgcc gatcgcggtc gggccgacga tcaccgatcc
     4081 gcaggcggga gcgcgatcgc cggtcacccc gggcagcccc gggacctggt cgacgaacgt
     4141 gacacagacc gtgtgcaggc cgccgccgac accgatggtc gactggaacc cgcggttctg
     4201 cccgaccccg gggaaggcgg caccgacgtc ggggcgagac tgattggcgg tcacgacggt
     4261 gggcgcaccg tcgacggcca cgcgcacggt caccgggttc gacgagtcgg ggtcgaacgc
     4321 ccaaccgctc acggtgaccg ctgaaccctg cggttggacc ccttcgatgt tgaacgcggg
     4381 ccggtgatcg accaccgaga cggtgcgaca gccgaggaac tgatcggcgc cgatcccgac
     4441 gttgacggcc gtcacgcaga tctcgtggac gccgggcacc gtggccgcca aggcgaccgc
     4501 gtagccggcc cgggggtcga gaccggtggt cgcggccacg tcgggacgcg acccggtcgc
     4561 ggcgaccgtc gtccggaccg cgtcgtcgac ggcaccgacc actcggtcgg tcacgacgac
     4621 gttgatcggc gtgcgcggcg acggatctcc cgcccacccc gagacgacga tggcgtccgg
     4681 ggcgacgctg accgcgtcga tcactcccga cgggctggtc gcggggacgt cgacgtcggt
     4741 acaaccgatc gtggcgtcgg cgccccggtt gtagttgcgc gcgatggcgc acactcgccg
     4801 ccgcccgggg gcgacggcca cgtcggccgt gaagccgtgg cccgtgccca ggccgggaaa
     4861 ggtgctggcg aggtcggggc ggccggccga tgcgagcacc cggccggcgg gggcgccgtc
     4921 gacggtgagg tcgacggcga gcgctgcgct cgtctcacga tcgagcgccc agccggtgac
     4981 ctggagccgg tcgcccgacg ggcggagcgt cgctccctcg aggttgccct gcgggtcggc
     5041 cgactccacg ttgccgaaca ccttgcagcc gagcaggcgg aacccggtgc ccgggccgac
     5101 gttcagcgcg gcgacgcaca tcgtgtgtgg gcccggcgtg acggggattg cgacgaggta
     5161 actgcggaac ttctccccgt aggaccccgg caggaacggg ttgtagacgc cagcggtacg
     5221 cacgaagagc ccgcggtcga cggcgacgac cacctcgatc gggcggttga catcgggatc
     5281 cttcgcccac ccgaccacac cgatcatccc cggttcggtc caggccaggt tcatctcgcc
     5341 gtatgggttc cgcgattcgg ccgacggcgc cggtcgtcgc gccgagccgg tcgacgcacc
     5401 ggcctcgtcg ggatcggaga cgagcagcgc cagtgcgctg aagcccacga cgaacaggag
     5461 ggcgacggcg gcaaactgcg ccacggcgcg tcggggcaac atctcggaat tcataccaga
     5521 ccggccgagt cgccggacat cgccccccgt cagaggtact ggcccgtcgc cactcgctcg
     5581 atcgcccgac caccttcgaa ccggttgtcc tcacggtgca ccagcgtgcg gatgtagacg
     5641 atccgctcac ccttcttgcc ggagatcttc gcccagtcgt cggggttggt ggtgttcggg
     5701 aggtcctcgt gttccttgaa ctcctggtgg atcgactcga ggagatcgtc gaccttgatg
     5761 cccttggcac cgtcggccag ctcacgcttg atggcgagct tcttggcccg acgcacgacg
     5821 ttctcgatca tggcgcccga cgagaagtcc ttgaagtaca tgacctcttt gtcgccgttc
     5881 tggtacgtca cctcgaggaa ctggttgttc tcgtcggtgc ggtacatctc ctcgacggtc
     5941 gtctcgatca tcacccgcac ggccttgtcg acgtcgccgc ctccgagggt catcacctcg
     6001 gtcgggtcga tcggcaggtc ggacgtgagg tactggctga agatctgcgc cgccgagtcg
     6061 gcgtcgggac ggttgatctt gatcttcacg tcgaggcggc ccggccgcag gatggccggg
     6121 tcgatgaggt cttcacggtt ggacgcaccg atcacgatga cgttgcgcaa cgtctcgaca
     6181 ccgtcgatct ccgccaacaa ctgcggcacg atcgtcgact cgatgtcgct cgagatgccg
     6241 gtgccgcggg tacggaacag cgactccatc tcgtcgaaga agatgatcac cggcgtgccg
     6301 tcggccgcct tgtcacgcgc ccgctggaac acctcgcgaa tgtgccgctc ggtctcgccc
     6361 acgtacttgt tgagcaactc cggccccttg atggagatga agtagctgtt ctggtcgcga
     6421 ccggtcatct cggcgacctt cttggcgagg ctgttggcga ccgccttggc gatcagcgtc
     6481 ttgccgcacc cgggaggccc gtacagcagg atgcccttcg gagcgggcag cttgtactcg
     6541 ctgaacttgg cctggtggag gaacgggagc tcgacggcgt cggtgatctg ctcgatctgg
     6601 tcgtcgaggc caccgatgtc gctgtaggcg atgtcgggca cctcttcgag catcagctcg
     6661 tcgacctcgg gccgcggcag cttctcgagg agcaccgaac tgcgggtgtc catgagcatg
     6721 acgtcgccgg cacggaggcg ctggcttcgc agggcgtcgc ccaactcggc cacacgctcc
     6781 tcgtcggcgc gcccgacgat cagcgcacgg cggccgtcct cgaggacctc gcgcagggtc
     6841 acgacctcgc ccacgatctc ctggctgcgc accatgacca ccgtgaacga ctcgttgagc
     6901 acgacctcga caccgcaccg gagttcgctc gcctcgatcg acggggacac ctcgacgcgc
     6961 atcttgcgac ccgagctgac gatgtcgacg gtgccgtcgt cgttgatccc gagcagcgtg
     7021 gcgtaggccg ccggcggctg ggtgagcttg tcgacctcgt cgcggagggc cgcgatgtgg
     7081 tcgcgcgcct cgcggagggt gtagctcagc ttctcgttct gcgcgacggc ctgcgccagc
     7141 tggcccttgg tctcgaggag tcgctcctcg agcgtgcgca cccgcttcgg cgcgtcctgg
     7201 atgcgacgga gcagggtgga cacctcgccc tggagctcgg ccacccggtc gcggaggtcg
     7261 gtgacctcgt cgccactcga ctggcgaagc tcgtcgccga tcggatcgcc ccaggggcgg
     7321 tcgccgacct cgtcgtgggt gccgttggat tcgtcgtgag cgtcgatcgg aaccacctcc
     7381 tcggaacgtt ggaacctaac accgcgatct tcggattcca cctcggttga cgcgatcgtc
     7441 acacgccgtt cgtcgtgccg ttttggcgac gacgacgtgc caagagcgac accgcgattc
     7501 tgcccggggt tggtctggga gcaggtggat ggggctacac tcacggcacg tggcgtcggg
     7561 ccccgaagcg cccgcaccga atccctgagg agcttgcaac atgggtatga aggtctggat
     7621 cgatcaggat ctgtgcaccg gcgatggtct gtgcgaagag atcgcaccca aggtgttcac
     7681 cctcctcgac gacggcttgg cctacgtggt cgacccggcc ggcaaggtct actccgatcc
     7741 gggtggcccc gagggtctcg ccgaggtgcc cgagggcgag tgggaaggca ccatcgaatc
     7801 cgccgaggag tgccccggcg agtgcatctt catcgagatg gagtagtcgg aaccgactga
     7861 gttcttcgag cgacccgggc ccgtgtgccc gggtcgttcc gcgtccagtc gccggtcagt
     7921 cgacgctgtc ggcgaaggtg atgggaggcg aggcgaggag cc
//
LOCUS       DAOSOA010000131         7750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9402_length_7750_cov_5.999090, whole genome shotgun sequence.
ACCESSION   DAOSOA010000131 DAOSOA010000000
VERSION     DAOSOA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9402_length_7750_cov_5.999090"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="PKY13_09445"
     CDS             <1..1216
                     /locus_tag="PKY13_09445"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOT80199.1"
                     /translation="GPSGDCGELTTTTDSNGYYEFTDLPPATGTITFGPEPDTIVTAR
                     HVGSDTSVDSDADPATRSLPFVVRSDEHVQHLDEGTYQGVSVTGFAWSDRDHNGVRSA
                     EEPPRPGITVTLVPAAANGSLSGLTAVTGNGSSTERTAVTGDDGAVSFVGLPPGRYAL
                     RYSSDAANRLTLRDVGTDDTADSDADPATGATAEVAAADGTTVHPGDAGYWAPTNVAI
                     AKDVLDGPDADGNVTYRLRVTNTGDTPTLGAITVIDDLPDGLVGVSVEGGGWTCDLTA
                     GALGCTTSTSLAPGQYHDIRLVARVAAGTTGPVVNRAVVRTGDDADNASTTDDDDTAA
                     VEVKPATAEAPSTPVPKPSPKPAPTPPTVPHERLAFTGGDPRRIVTVALAAVIVGVVL
                     RRRSRKKRPSAS"
     gene            complement(1308..2117)
                     /locus_tag="PKY13_09450"
     CDS             complement(1308..2117)
                     /locus_tag="PKY13_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT80200.1"
                     /translation="MATFVFFHAHPDDECIQTGGTMAKVAADGHRVVLVLATRGELGE
                     VPDGFLDEGEQLGLRRIAETYASADVLGADRVEFLGYVDSGMIDTPGNDGPYCFWRAN
                     VEAAAGRLAAILREEAADVLTIYDDNGGYGHPDHIQVHRVGARAAELAGTPGVFEATM
                     NRDDIIRSMRESSEYLTEEQRAEMPDLEGEDAENFGVDEARITHAVDVTDFTDAKRAS
                     MRCHRSQIADEAFFLAMPDDAFARAFGTEWYISHGHTRGAGDPFLTDLLAE"
     gene            complement(2117..3265)
                     /locus_tag="PKY13_09455"
     CDS             complement(2117..3265)
                     /locus_tag="PKY13_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOT80201.1"
                     /translation="MGPLSGLKIVEVAGIGPGPFAAMMLADMGADVIRVDRSAQVRGG
                     DPALPPGDTLNRGRRSIGLDLKSPDGVETLLQLVERADGLIEGFRPGVAERLGFGPDV
                     ALARNPKLAFGRMTGWGQDGPYALTAGHDINYIALAGVLDTFSRAGGQPTPPINLVGD
                     FGGGGMFLAYGMVCAILSAKLGGTGQVVDAAMVDGSATLMSMIWAFKGMGIWESDAPG
                     TNLLDTGAHFYDTYRCADGRWVSIGSIEPQFYAELLRYTGLEGEELPSQMDKTQWGSM
                     KEKLAEIFATKTRDEWSAIMEGTDVCFAPVLSMDEATRHPHNVQRGTFIELDGVTQPA
                     PAPRFSATPGGVQRPPAHAGQHTDEVLSEWLGLDDAGIALLRESGTVA"
     gene            complement(3325..4167)
                     /locus_tag="PKY13_09460"
     CDS             complement(3325..4167)
                     /locus_tag="PKY13_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT80202.1"
                     /translation="MPSAPVNGIEIYYDEFGAVDDPVLVLMAGLGGQLLNYPDEFCLA
                     FVDRGMRVVRFDNRDAGLSTHLPDGTSYTLDDMAADVFGLLDHLGVAQAHVWGSSLGG
                     MIAQTMAIAHPERVRSLISVQSTTGEPGVGQPDPTALAEIIESTQPSSGREEAIAKAA
                     RLYEVLQNNPAVTDPEALRIRAEANYDRSYDPAAGARQVGAVLLATPRAEALAVLDVP
                     TLVIHGNRDPLIDISGGRRTAELVPGATFLEVDGMGHDLAPVFWSQYVDAVVAHVAAV
                     ETAG"
     gene            4238..4753
                     /locus_tag="PKY13_09465"
     CDS             4238..4753
                     /locus_tag="PKY13_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80203.1"
                     /translation="MRLKLALAITGLALAITACFPDQGGSPTPTTVSTTAAPTTTSTT
                     TTTAPLPTLTYTGTADKTAGCYSGLLGDDTFEQSLGTDGWWHFGFQGCTLFSTETVAL
                     IDPAGGPTGDAFQSPTGYQCRFEIVGTSDATGYATNQIGNVADTGGLIFLESSSSWSS
                     PTTWKITCIQA"
     gene            4789..5697
                     /locus_tag="PKY13_09470"
     CDS             4789..5697
                     /locus_tag="PKY13_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT80204.1"
                     /translation="MADIVFGVHTGPSNTTTAELISLWRHIEDGPFDWISIWDHFYGA
                     DGTSPDNLEAVAMHTALAMTTTRVTCGSLVYCAGYRHPAVLANAMATIDHLSGGRCAF
                     GIGAGWATFEYAAYGIPYDRAGVRLDVMEESLRCVQGLLHHGADHRFDFAGQHFTMTD
                     AACVPAPVQPHLPLWVGGAGERRTLPIAAKYADGWNVPFISPGQFARKNALLDELCER
                     EGRDPSSVARAVNVGYAVDAASIASQFGPLAEALAPGVLTGSTDEIAEGVGRFINAGA
                     TQINLALRAPFSLEAIDALATYIESR"
     gene            complement(5706..6194)
                     /locus_tag="PKY13_09475"
     CDS             complement(5706..6194)
                     /locus_tag="PKY13_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT80205.1"
                     /translation="MTTRVLGPDDVTTYRSIRLEALRSDPAAFCSTHEREAAFDDATF
                     AERLAGYAGRPGVVIVDDAADPAGMVGIGEAEAGGAAMMWGMWVRPTERRSGLGARLV
                     EAAVAWADEAGLTAVDLWVVVGNGAAISLYERCGFLATGAADVVPGRPGVAEVEMRRK
                     LR"
     gene            6271..7284
                     /gene="galK"
                     /locus_tag="PKY13_09480"
     CDS             6271..7284
                     /gene="galK"
                     /locus_tag="PKY13_09480"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOT80206.1"
                     /translation="MDIRAWAPGRVNLIGEHTDYSGGLVFPMAIQLGTTVEGDLLDGE
                     IRLRSDDQPDPLHLTLPVVDPAAVTPAWGRYVAGVASELAVETGLDGRVASNLPAGAG
                     LSSSAALEVAVALALGAADEYSPDEIAAVAQRAEFAATGVPCGIMDQLASARGVAGHA
                     LRIDCTTFEVTAVPVGDDVAVVVIHSGQERQLADSAYAERRAQCEAAAAIVGPLRQAT
                     LESLDAIEDSVVARRARHVISENGRVDEFAAALAAGDHAAAGAAMAASHASLRDDFDV
                     STPVLDELVEHLRGVDGVHGARLTGAGFGGCVVALCRPGVDPTPPSLRGWVVRPSDGA
                     HLR"
     gene            complement(7341..>7750)
                     /locus_tag="PKY13_09485"
     CDS             complement(7341..>7750)
                     /locus_tag="PKY13_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOT80207.1"
                     /translation="DVQYVDRSGERIALHGANEVEGIINVVAPYGAFQRSDLEPEVPA
                     GDPIPGRTEVTGLRVGGYPITYGASTLMVVGFDDDGPIGRGLLTYGQSGDAASEHHAD
                     QMREFSAKRLRPLRFDEDDIVADPEYWTMTLHG"
BASE COUNT         1126 a         2846 c         2663 g         1115 t
ORIGIN      
        1 cggacccagc ggcgattgcg gtgaactcac cacgacgacg gactcgaacg gctactacga
       61 gttcaccgat ctgcccccgg cgaccggcac gatcacgttc ggtcccgagc cggacaccat
      121 cgtcaccgcc cggcacgtcg gttcggacac ctcggtcgac tccgacgccg accccgccac
      181 ccggtcgctc ccgttcgtgg tccgctccga cgaacacgtc cagcacctcg acgagggcac
      241 ctaccagggc gtctcggtca cgggcttcgc ctggtcggat cgcgaccaca acggtgtcag
      301 gtcggccgag gagccgccga ggccggggat cacggtgacg ctggtcccgg ccgccgccaa
      361 cggcagcctg agcggcctca cggccgtcac gggcaacggc agctcgaccg agcgcacggc
      421 cgtcaccggt gacgacggcg ccgtcagttt cgtcggcctg ccgccgggac gatacgccct
      481 gcgctacagc tccgatgccg ccaaccgact cacgctccgc gacgtcggga ccgacgacac
      541 ggctgactcc gacgccgatc cggcgaccgg tgcgaccgcc gaggtcgccg ccgccgacgg
      601 caccacggtg caccccggtg acgccggcta ctgggcaccc accaacgtgg ccatcgccaa
      661 ggacgtcctc gacgggcccg acgccgacgg caacgtgacc taccgcctgc gggtgaccaa
      721 caccggtgac accccaaccc tcggcgccat cacggtcatc gacgatctcc ccgacggtct
      781 cgtcggggtc agcgtcgagg gcgggggctg gacctgcgac ctcaccgccg gggccctcgg
      841 ctgtacgacg tcgacatcgc tcgccccggg gcagtaccac gacatccggc tcgtggcccg
      901 ggtcgctgcg ggcacgaccg ggccggtcgt gaaccgggcc gtggtccgca ccggtgacga
      961 tgccgacaac gcctccacca ccgacgacga cgacaccgca gcggtggagg tcaaaccggc
     1021 caccgccgag gccccgtcga cgccggtgcc caagccgtcg cccaagccgg cgccgacgcc
     1081 tccgacggtt cctcatgagc gcctggcgtt caccggcggc gacccgcgcc gcatcgtcac
     1141 cgtggcgctg gccgcagtga tcgtgggtgt cgtgctccgc cggcggtcac gcaagaagcg
     1201 ccccagcgcc tcctgaccgg cgacgggtcg cccgtcagcc gagcgaccac tcgactgccg
     1261 ccaacgcgtg cagtcgagtg gtcgcggcgg cgcgcttcga ctcggggcta ctcggcgagg
     1321 aggtcggtga ggaacggatc gccggcccca cgggtgtggc cgtggctgat gtaccactcg
     1381 gtgccgaacg cccgggcgaa cgcgtcgtcg ggcatcgcca ggaagaaggc ctcgtcggcg
     1441 atctggctgc ggtggcagcg catcgacgcc cgtttcgcgt cggtgaagtc ggtgacgtcg
     1501 acggcgtggg tgatgcgggc ctcgtcgaca ccgaagttct cggcgtcctc gccctcgagg
     1561 tcgggcatct cggcgcgctg ttcctcggtc aggtactcgc tcgactcccg catgctccgg
     1621 atgatgtcgt cgcggttcat ggtcgcctcg aacacgccgg gggtgccggc gagctcggcg
     1681 gcgcgggcac cgacccggtg cacctggatg tggtcggggt gcccgtagcc gccgttgtcg
     1741 tcgtagatcg tgagcacgtc cgccgcttcc tcacgaagga tggccgccag tcgccctgcc
     1801 gcggcctcga cattggcgcg ccagaagcag tacgggccgt cgttgccggg ggtgtcgatc
     1861 attccggaat cgacgtagcc gaggaactcg acgcggtcgg cgccgagcac gtcggccgac
     1921 gcgtaggtct cggcgatccg gcggaggccg agctgctcgc cctcgtcgag gaacccgtcg
     1981 ggcacctcgc cgagctcgcc gcgggtcgcg agcaccaaca ccacccggtg cccgtccgcg
     2041 gccaccttgg ccatggtgcc gccggtctgg atgcactcgt cgtcgggatg ggcgtggaag
     2101 aagacaaacg tcgccatcag gcgaccgtac ccgactcccg cagcaaggcg atcccggcat
     2161 cgtccaggcc gagccactcc gacagcacct cgtcggtgtg ctggccggcg tgggccgggg
     2221 gccgctgcac cccgcccggc gtcgccgaga agcgtggcgc cggtgccggc tgggtcacgc
     2281 cgtcgagctc gatgaacgtc ccacgttgca cgttgtgggg gtggcgagtc gcctcgtcca
     2341 tcgagagcac cggagcgaag cacacgtcgg tgccttccat gatggcgctc cactcgtcgc
     2401 gggtcttcgt ggcgaagatc tccgccagct tctccttcat cgagccccac tgcgtcttgt
     2461 ccatctgcga cggcagctcc tcgccctcga ggcccgtgta gcggagcagc tcggcgtaga
     2521 actggggctc gatcgacccg atcgacaccc agcggccgtc ggcacaccgg taggtgtcgt
     2581 agaagtgggc ccccgtgtcg aggaggttgg ttcccggcgc gtcggattcc cagatcccca
     2641 tgcccttgaa cgcccagatc atcgacatga gcgtcgccga gccgtcgacc atcgcggcgt
     2701 cgacgacctg tccggtgccg ccgagcttgg ccgagaggat cgcgcacacc atgccgtagg
     2761 cgaggaacat gccaccaccg ccgaagtcgc ccacgaggtt gatgggcggg gtcggctggc
     2821 cgcccgcccg gctgaacgtg tcgagcacac cggcgagcgc gatgtagttg atgtcgtggc
     2881 cggcagtcag cgcgtacggg ccgtcctggc cccacccggt catccgcccg aacgcgagct
     2941 tcgggttacg cgccagcgcg acgtcgggac cgaacccgag acgttcggcg actccgggac
     3001 ggaagccctc gatgaggcca tcggcccgct cgaccagctg caggagcgtc tcgacgccgt
     3061 cgggcgactt gaggtcgagc ccgatcgaac gccgcccacg gttgagcgtg tcgccgggcg
     3121 gtagggccgg atcgccgccg cgaacctgcg ccgaccggtc gacgcggatc acgtcggcgc
     3181 ccatgtcggc caacatcatc gccgcgaacg gacccgggcc gatgccggcc acctcgacga
     3241 tcttcaaccc tgacaacggt cccatggcga tcctgttccc cggccgcggc cggtccttcg
     3301 tagacggatg gtgtgtggcg ggcttcagcc cgcggtttcg acggccgcga cgtgcgcgac
     3361 gacggcgtcg acgtactgac tccagaacac cggcgccaga tcatggccca tgccgtcgac
     3421 ctcgaggaac gtcgccccgg ggacgagctc ggcggtgcgg cgcccgccgg agatgtcgat
     3481 cagggggtcg cggttgccgt ggatcaccag cgtgggcacg tcgagcaccg ccagcgcctc
     3541 ggcgcggggc gtcgcgagca ggacggctcc gacctgccga gcgccggccg cggggtcgta
     3601 ggagcggtcg tagttggcct cggcccggat gcggagcgcc tcgggatcgg tgacggcggg
     3661 gttgttctgc agcacctcgt acagccgtgc cgccttggcg atcgcttcct cgcggcccga
     3721 cgacggttgt gtcgactcga tgatctcggc gagggccgtc ggatcgggtt ggccgacacc
     3781 cggttcgccc gtcgtcgact ggaccgagat gaggctgcgc acccgctccg gatgggcgat
     3841 cgccatcgtc tgggcgatca tgccgccgag cgaactgccc cagacgtgcg cctgtgcgac
     3901 accgaggtgg tcgaggagtc cgaaaacgtc ggcggccatg tcgtcgagcg tgtacgacgt
     3961 gccgtcgggc aggtgggtcg acagaccggc gtcgcggttg tcgaaccgga cgactcgcat
     4021 gccccgatcg acgaacgcca gacagaactc gtcgggatag ttgaggagct gaccgccgag
     4081 cccggccatg aggacgagca ccggatcgtc gaccgccccg aactcgtcgt agtagatctc
     4141 gatcccgttc acgggggcac tgggcatggg gaggcatcgt actgacccgc cccgggcgat
     4201 gatgcgtccc tcactcacga cgcgttacgt tctttcgatg cgtttgaaac ttgcacttgc
     4261 catcaccggc ctcgctctcg ccatcaccgc ctgcttcccc gaccagggcg ggtcacccac
     4321 cccgacgacc gtctcgacca cggcagctcc cacgaccacg agcaccacga cgacgacggc
     4381 gccgctcccg acgctcacct acaccggcac cgccgacaag acggcgggct gctattccgg
     4441 gctgctcggc gacgacacct tcgagcagtc gctcggcacc gacgggtggt ggcacttcgg
     4501 gttccagggc tgcacgttgt tctcgaccga gaccgtggcg ctgatcgacc ccgccggcgg
     4561 gcccaccggc gatgcgttcc agtcgccgac gggctaccag tgccgcttcg agatcgtcgg
     4621 cacgagcgac gccacggggt acgccaccaa ccagatcggc aacgtcgccg acaccggcgg
     4681 cctcatcttc ctcgagtcgt cgagctcgtg gtcgagcccg acgacctgga agatcacctg
     4741 catccaggcg tagccgtccg tgggtgaccg tggcggtact gtcggcccat ggccgacatc
     4801 gtcttcgggg tgcacaccgg gccgtccaac acgacgaccg ccgagctgat ctcgctctgg
     4861 cgccatatcg aggacggccc gttcgactgg atcagcatct gggaccactt ctacggggcc
     4921 gacggcacct cgccggacaa cctcgaggcg gtcgcgatgc acacggcgct cgccatgacc
     4981 accacgcggg tgacgtgtgg gtcgctcgtg tactgcgccg gctaccgcca ccccgcggtg
     5041 ctcgctaacg ccatggcgac catcgaccac ctgtcgggcg gccggtgcgc gttcggcatc
     5101 ggggccggat gggcgacctt cgaatacgcc gcgtacggca tcccctacga ccgggcgggc
     5161 gtccgactcg acgtgatgga agagtcgctg cgctgcgtcc agggcctgct ccaccacggc
     5221 gccgaccacc ggttcgactt cgcgggacag cacttcacga tgaccgacgc cgcgtgcgtc
     5281 ccggccccgg tccaaccgca cctgcccctg tgggtcggtg gcgccggcga acggcgcacg
     5341 ctgccgatcg cggcgaaata cgccgacggc tggaacgtgc ccttcatctc ccccggccag
     5401 ttcgcccgca agaacgccct cctcgacgaa ctgtgcgaac gcgaggggcg ggacccatcg
     5461 tcggtcgccc gggcagtgaa cgtcgggtat gccgtcgatg ccgcgtcgat cgcgtcgcag
     5521 ttcggtcccc tcgccgaagc gctcgcaccc ggcgttctca ccggttcgac cgacgagatc
     5581 gccgagggag tcggccgctt catcaacgcc ggagccacgc agatcaacct cgcgctgcgg
     5641 gcgccgttct ccctcgaggc gatcgacgcg ctggccacct acatcgaatc ccgatagcgc
     5701 cggcgtcagc gcagcttgcg gcgcatctcg acctcggcga cgccggggcg acccggcacc
     5761 acgtcggccg cgcccgtcgc gaggaatccg cagcgctcgt acagggagat cgccgctccg
     5821 ttgccgacga cgacccagag gtcgaccgcc gtcaggcccg cctcgtcggc ccacgcgacc
     5881 gccgcctcga cgagccgggc gccgaggccg gagcgacgct ccgtcgggcg gacccacatc
     5941 ccccacatca tcgccgcacc cccggcctcg gcctcaccga tgccgaccat ccccgccggg
     6001 tcggcggcgt cgtcgacgat gaccaccccg ggccgtcccg cgtagccggc gagacgctct
     6061 gcgaacgtgg catcgtcgaa cgccgcctcg cgttcgtggg tcgagcagaa ggccgccgga
     6121 tcggaccgga gcgcctccaa ccggatgctc cggtacgtgg tcacgtcgtc ggggccgagc
     6181 acgcgcgtcg tcatggacgg gaccgtagcg aggtaccccg tcgcgggtac ggcgaatatc
     6241 ggtcccggca ccccgggtag cctcgatccg gtggacatca gggcgtgggc accggggcgg
     6301 gtcaacctga tcggcgagca caccgactac tccggcggcc tggtgttccc gatggccatc
     6361 cagctcggca cgaccgtcga gggcgacctc ctcgacgggg agatccgtct gcggtccgac
     6421 gaccagcccg acccgctgca cctcaccctg ccggtcgtcg acccggccgc ggtgacaccc
     6481 gcctggggtc ggtacgtcgc gggcgtggcg tccgaactgg ccgtcgagac cgggctcgac
     6541 ggtcgagtgg cctcgaacct cccagcgggt gccgggctct cgtcgtccgc cgcgctcgag
     6601 gtggccgtgg ccctcgcgct cggtgccgcc gacgagtact cccccgacga gatcgcggcg
     6661 gtcgctcagc gagccgagtt cgccgccacg ggcgtgccgt gcgggatcat ggaccagttg
     6721 gcgtcggcgc ggggcgtcgc cggtcacgcc ctccggatcg actgcacgac gttcgaggtc
     6781 accgccgtgc cggtcggcga cgacgtcgcc gtggtggtga tccactcggg gcaggagcgt
     6841 cagctcgccg attcggccta cgccgagcgt cgggcccagt gcgaggccgc cgcagcgatc
     6901 gtcggaccgc tgcggcaggc gaccctcgaa tcgctcgacg cgatcgaaga ctccgtcgtg
     6961 gcccggcggg cccgacacgt gatctccgag aacggacgcg tcgacgagtt cgccgcagcg
     7021 ctcgctgcgg gcgaccacgc cgccgccggc gcggcgatgg ccgccagcca cgccagcctg
     7081 cgcgacgact tcgacgtctc gacgccggtg ctcgacgaac tcgtcgagca cctgcggggc
     7141 gtggacgggg tccacggcgc ccgactcacg ggagcggggt tcggcggctg cgtcgtcgcg
     7201 ctgtgccgcc ccggcgtcga ccccacgccg ccctcgttgc gaggttgggt cgtgcggccg
     7261 tccgacggag cgcacctgcg ctgatcacgc cggtgcgcgc gagcgccaga gcgccggagc
     7321 gccacgccag ccgtcagacc tcagccgtgg agggtcatcg tccagtactc cgggtcggcg
     7381 acgatgtcgt cctcgtcgaa tcggagtggc cgaaggcgtt tcgccgagaa ctccctcatc
     7441 tggtcggcgt ggtgctccga ggcagcgtcg cccgactgtc cgtaggtgag gaggccgcgg
     7501 ccgatcggcc cgtcgtcgtc gaacccgacg accatgagcg tcgacgctcc gtaggtgatc
     7561 gggtagccgc cgacccgcag gccggtgacc tcggtgcgcc cggggatcgg gtcgccggcc
     7621 ggcacctcgg gctcgaggtc gctccgctgg aaggcaccgt acggagcgac gacgttgatg
     7681 attccctcga cctcgttggc cccgtggagt gcgatgcgtt cgccactgcg gtcgacgtac
     7741 tgcacgtcgc
//
LOCUS       DAOSOA010000132         7684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9514_length_7684_cov_4.310001, whole genome shotgun sequence.
ACCESSION   DAOSOA010000132 DAOSOA010000000
VERSION     DAOSOA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9514_length_7684_cov_4.310001"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKY13_09490"
     CDS             complement(<1..534)
                     /locus_tag="PKY13_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80208.1"
                     /translation="MTKATLDEMVVRAPAPEPAVAPALLRRRRLLVVLLLVVTGVSMV
                     MAGGHSYAIDNEVQFQTTRSLVNLRPDLTELDDGWAARDDGPYRVRDDGTRVAIVPIG
                     QSILSAPFYVASRAVAQLAPSYQRDQFVRTGTFFTNSLLLGLTAVVVALLAIELGATD
                     RHGILLGYVYGLGTYALP"
     gene            complement(531..1730)
                     /locus_tag="PKY13_09495"
     CDS             complement(531..1730)
                     /locus_tag="PKY13_09495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT80209.1"
                     /translation="MRIVVVNNFFPPRVGGSSHLSALLAKHYVEAGHEVLVITGAYAD
                     APETEERHGYRIVRLPVARMPQLGLSIDFDITFAARWSNMRRIKKLLREFCPDVIHQH
                     GQFLDLSWMTGRWAAKNDVPVLLSIHTRLENPNRVYALLFAALDYLVVRPLVNYYDPH
                     MVVMDRLMDAYIRGRYGTGEARLVDIPVGVDLTQFKVPGDGARVREQWGLGDKPVIVS
                     LGHVIPLRDRVLLIEALPGILRQHPETMVVVVGKVHHDAFLRLANTFGVEDHVLCVGP
                     VPPSEVPDYLAAGQVETHDLQGFGLGTASLEAMAAGTPIVAAVRMDNFRGIELRNGEN
                     IVLVSEGRPAELCGAISQILSDPAGAQLIAKEQVDLAERCFSIEAVTQAHLDTFERLV
                     DQRRSAR"
     gene            complement(1727..2164)
                     /locus_tag="PKY13_09500"
     CDS             complement(1727..2164)
                     /locus_tag="PKY13_09500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT80210.1"
                     /translation="MTDPAPHPYHPSVWLTGDPEIGAGTWIGAFCVVDGSGGLRIGAG
                     CDISAGSQIYTHSSAKRCVSGRSYPTVDRAPTVIGDHVFIGAGAIVNMGVTIGSGAVI
                     GAGAVVTADVAPGTIVAGVPAREIGRVEIHGDRVEFVHGGADR"
     gene            complement(2168..3112)
                     /locus_tag="PKY13_09505"
     CDS             complement(2168..3112)
                     /locus_tag="PKY13_09505"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT80211.1"
                     /translation="MTTPRTVLFTGGAGFIGMHVMPLLLERGYKVRLFDSMTHRANRD
                     EIDALIATGDVELIEQDVRYGGAIERAIKGCESVIHFATVSINKSIADPDESIDINMV
                     GNHNVIAAAANAGVRRLVFASSASVYGDPPSLPMAEDGPLNPLTPYCIGKLTGEQLCG
                     FYERQKQLSWIALRFFNVYGEGQKTSAYYTSVINHFVNRIANGEAPVIDGAGEQSMDF
                     VHVTDIARSVVAALDAERSNLPINIGTGIPTSVAQLAKILIQAVGADVEPIFNPREVL
                     VSRRSADISRAREILGWEPTISVEDGMSALARAAIGQR"
     gene            complement(3150..4289)
                     /locus_tag="PKY13_09510"
     CDS             complement(3150..4289)
                     /locus_tag="PKY13_09510"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT80212.1"
                     /translation="MPDQIALGQPTTGPEELEAVRKVLESGWVAGQGPMSREFEAAFA
                     ARVKAPHALAVNNCTAGLHLALLAKGVGPGDEVIVADYTYPATGHAVLYCGARPRFAD
                     VRPDTALVDPAAVEALVGERTVGILAVDTFGQCADYDELNAIASAKGLFVIEDAACAA
                     GATYRGKPAGHPDLADLAAFSLHGRKGITSGEGGVVTTGDAEAAAFVGKHLCFGVESA
                     LARAGSNDLAVPSFDQLGYNYKLSDIASAIALVQLDRLEGLLDTRRKIAHEYAQLLSE
                     CELLDLPVELEGREHPWQAFVLTLDPSVSRGEMAKSLREAGIGCNIGTFACHLQPVYG
                     VTDACPVSADLFGRHLAIPMHANLTQDQVERVATAVIDAATASRS"
     gene            4479..5579
                     /locus_tag="PKY13_09515"
     CDS             4479..5579
                     /locus_tag="PKY13_09515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT80213.1"
                     /translation="MIPISVVDLGPGAEELVQQVLKSGHLAQGARVAEFEARFAELTG
                     VTHAVAVNNGTTALVAALRVLELEPGDEVITTPFTFVATLNAIVDAGASVRFADIADA
                     DFNIDPALVEAQLTDRTRVLLPVHLYGQCADLDRLDPIVAANDLQLVEDSAQAHGATI
                     GSRAAGATGLGCFSFYATKNITTGEGGMITTDDDRLADRLRILRNQGMRSRYQYEMAG
                     NNYRLTDLQAVLALPQLDGYATNVARRRANAAALSAGLAGIDGVVTPSELPGRGHVWH
                     QYTIRITGDAPVSRDTVLERLQADGIGCGVYYPKVVFDYDCFAGHPGIHTADLPVARR
                     LAGEVLSLPVHPALTDAELDRIVEGVRDAVSA"
     gene            5579..6553
                     /locus_tag="PKY13_09520"
     CDS             5579..6553
                     /locus_tag="PKY13_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT80214.1"
                     /translation="MAGVRIGLVGAGSMGSLHARVVSESPRADLAWIADPSRPAGEAA
                     ATRFGTTWLPHLRTDDVDAVVIAAATEAHPKLADEVLDAGIPLLVEKPLADDIVDTRR
                     LVERSAAAGIPLMCGFLERYNPAIRTALDFVEHPLHATAIRHSPYVPRIRTGVASDLL
                     IHDLDTMLRLFGQFPDSASGTAGYFHPDSDPRSEDVADAVLRFSNGSIGAASASRISQ
                     RKVRTLVIGELDRQIELDLVRQDITIYRHLGNAPTDEESGLGYSQQTVIDIPVIRHQR
                     EPLASQLDRFLDLIDGTGDPDEERATLLPPHELLATIRASIDDAATRA"
     gene            6546..>7684
                     /locus_tag="PKY13_09525"
     CDS             6546..>7684
                     /locus_tag="PKY13_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80215.1"
                     /translation="MPESGGTDGRPIDRIRQAMDLARSGQAAMTVGLLANGIASYVFL
                     AASGRALGPSRFAAVSVLWAVLFLVGSGLFQPFEQELGRSIAARRARGDGFGALVRRA
                     AVLAMALFAIVAVGIAIATRPIADALFRGDEGFVVALIVGIGGVGVMLVTRGLLAGSG
                     RYYGYAVLFFADAATKSIPALALAVAGVREPLVFAGVVAVSAYLGALVPLSKGTRLSA
                     PGPEPAWGPLSSSVGFLLLTSLLSQITLNIGTLAVEILATKSEHDRAGVFLSGLVIAR
                     VPLFLFQAVQAIVLPRLSRLAASGDLDGFRDNVRKLLAAMVGATALATAVSAAVGPIA
                     VKILFGEDYGLLGSRDMGMLTFASLLMMCALTINQAQIALHHQHQT"
BASE COUNT         1148 a         2725 c         2625 g         1186 t
ORIGIN      
        1 cgggagggcg tacgtgccga ggccgtacac gtagccgagc aggatcccgt gacggtcggt
       61 agcgccgagc tcgatcgcca gcagcgccac gacgaccgcg gtcagcccga gcagcagcga
      121 gttggtgaag aacgtgccag tgcggacgaa ctggtcgcgc tggtacgacg gcgccagctg
      181 cgcaacagcg cggctggcaa cgtagaaggg ggccgacagg atcgactggc cgatcggcac
      241 gatcgcgacg cgggttccgt catcgcggac gcggtacggc ccgtcgtcgc gtgccgccca
      301 cccgtcgtcg agctcggtca ggtcgggacg gaggttcacc aggctccggg tcgtctggaa
      361 ctgcacctcg ttgtcgatgg cgtagctgtg cccaccggcc atcaccatcg acacgcccgt
      421 gaccacgagc aggaggacga cgagaaggcg ccggcgccgg agcagtgccg gggcgactgc
      481 gggttcgggc gccggagcgc gcacgaccat ctcgtcgagg gtcgccttgg tcatcgcgcc
      541 gaacgtcgct ggtcgacgag gcgctcgaag gtgtcgaggt gcgcctgcgt caccgcctcg
      601 atcgagaagc accgttccgc gaggtcgacc tgctccttgg cgatcagctg cgcaccggcg
      661 ggatcgctca ggatctggct gatcgcaccg cacagctcgg cgggacgacc ttccgagacg
      721 agcacgatgt tctcgccatt gcgcagctcg atgccgcgga aattgtccat gcgcaccgct
      781 gcgacgatcg gggtgcccgc ggccatcgct tcgaggctcg ccgttcccag accgaacccc
      841 tgcaggtcgt gggtctcgac ctggccggcc gccaggtagt cggggacctc gctgggtggc
      901 accgggccga cgcacagcac gtggtcctcg accccgaacg tgttcgccag ccggaggaac
      961 gcgtcgtggt ggaccttgcc aaccaccacc accatcgtct cggggtgctg acggaggatg
     1021 cccgggagcg cctcgatgag caggacccga tcgcgcagcg ggatcacgtg gccgagggac
     1081 acgatgaccg gcttgtcgcc cagaccccac tgctcgcgca cccgggcccc gtcgccggga
     1141 accttgaact gcgtcaggtc gacgcccacc gggatgtcga cgagacgggc ctctccggtg
     1201 ccgtaccgac cgcggatgta ggcgtccatg agccggtcca tcacgaccat atgagggtcg
     1261 tagtagttca cgagcggtcg gaccacgagg tagtcgagcg ccgcgaagag gagggcgtag
     1321 acccggttcg ggttctcgag gcgcgtgtgg atgctgagga gcaccggcac gtcgttcttg
     1381 gcggcccaac ggcccgtcat ccaggacagg tcgaggaact ggccgtgctg gtggatcacg
     1441 tcgggacaga actcgcgcag cagcttcttg atccgccgca tgttggacca gcgggcggcg
     1501 aacgtgatgt cgaagtcgat cgacaacccg agctggggca tgcgggccac cggcagccgc
     1561 acgatccgat acccgtgacg ctcctcggtc tccggagcgt cggcgtacgc gccggtgatc
     1621 acgaggacct cgtgacccgc ctcgacgtag tgcttcgcga ggagcgccga gaggtgcgag
     1681 ctgccaccga cgcgaggcgg gaagaagttg ttgacgacga cgatcctcac cggtctgctc
     1741 ccccgtggac gaactcgacg cgatcgccgt ggatctcgac gcggccgatc tcccgcgccg
     1801 ggacgccggc gacgatggtc ccgggcgcca catcggccgt cacgaccgcc ccggcgccga
     1861 tcacggcgcc cgatccgatg gtcactccca tgttgacgat cgccccggcg ccgatgaaca
     1921 catggtcgcc gatcacggtc ggggcgcgat cgaccgtcgg atacgagcga cccgacacac
     1981 atcgcttggc ggaggagtgc gtgtagatct gggagccggc ggagatgtcg cagccggctc
     2041 cgatccggag gccgccggag ccgtccacca cgcagaacgc gccgatccag gtgccggcgc
     2101 cgatctcggg atcaccggtc aaccacacac tcgggtggta cggatgtggg gcgggatccg
     2161 tcacgcgtca ccgttggccg atggcagccc gcgccaacgc gctcatgccg tcctcgaccg
     2221 agatcgtggg ctcccacccg aggatctcgc gagcccggga gatgtcggcc gatcggcggc
     2281 tcaccaggac ctcgcggggg ttgaagatcg gctcgacgtc cgcgccgact gcttggatga
     2341 ggatcttcgc cagctgcgcc accgaggtcg ggatgcccgt cccgatgttg atgggcaggt
     2401 tcgagcgctc ggcgtcgagc gccgcaacga ccgaacgggc gatatcggtc acgtgcacga
     2461 agtccatgga ctgctcaccg gcaccgtcga tcacgggcgc ctcgccgttg gcgatgcggt
     2521 tcacgaagtg gttgatcacg gaggtgtagt aggcgctggt cttctgaccc tcgccgtaga
     2581 cgttgaagaa gcgcagtgcg atccacgaca gctgcttctg ccgctcgtag aagccgcaga
     2641 gctgctcgcc ggtcagcttg ccgatgcagt acggcgtgag cgggttgagg gggccgtcct
     2701 cggccatcgg gaggctgggc ggatcgccgt agaccgacgc gctcgacgcg aacacgaggc
     2761 ggcggacccc ggcgttcgcc gctgcggcga tcacgttgtg gttaccgacc atgttgatgt
     2821 caatgctctc gtcgggatcg gcgatgctct tgttgatcga tacggtcgcg aagtggatga
     2881 cgctctcgca acccttgatg gcccgctcga tggcgccgcc gtaccgcacg tcctgctcga
     2941 tcagctcgac gtcacccgtg gcgatcagcg cgtcgatctc gtcgcggttg gcccggtgcg
     3001 tcatcgagtc gaacaggcgc accttgtagc cgcgctcgag caggaggggc atcacgtgca
     3061 tgccgatgaa accggcgccg ccggtgaaga ggactgtacg aggcgtggtc atgtcggttc
     3121 ctgtcgggtc gaggaggtcg gaggtggcgt caggagcgcg acgccgtggc ggcatcgatc
     3181 accgcggtgg cgacccgttc cacctgatcc tgcgtgaggt tggcgtgcat cgggatggcg
     3241 aggtgacgtc cgaacaggtc ggccgagacc gggcaggcat cggtgacacc gtagaccggc
     3301 tgcagatggc aggcgaacgt gccgatgttg cacccgatgc ccgcctcgcg gagcgatttg
     3361 gccatctcgc cgcgggacac agacggatcg agcgtcagga cgaacgcctg ccaagggtgc
     3421 tcgcgtcctt ccagctcgac gggcaggtcg agcagctcgc actccgagag tagctgcgcg
     3481 tactcatggg cgatcttgcg gcgggtgtcg agcaacccct cgagacggtc cagttggacg
     3541 agcgcgatcg cgctagcgat gtcggagagc ttgtagttgt acccgagctg atcgaaggac
     3601 gggacggcga ggtcgttgga gccggcgcgg gcgagcgccg actcgactcc gaaacacagg
     3661 tgcttgccca cgaacgctgc ggcttcggcg tcaccggtgg tgacgacgcc gccctcgccg
     3721 gacgtgatgc ccttgcgccc gtgcaggctg aacgcggcga gatcggccag atcgggatgg
     3781 ccggccggtt tgccgcggta ggtcgcgccg gcggcgcacg cggcgtcctc gatcacgaac
     3841 aggcccttgg ccgacgcgat ggcgttgagc tcgtcgtagt cggcgcactg cccgaaggtg
     3901 tcgacggcga ggatgccgac ggtgcgctcg ccgacgagcg cctcgaccgc tgcggggtcg
     3961 acgagcgcgg tgtcgggccg gacgtcggcg aaccggggcc gtgccccgca gtacagcacg
     4021 gcgtggccgg tcgcgggata ggtgtagtcg gcgacgatca cctcgtcgcc ggggccgacc
     4081 cctttagcca ggagcgccag gtgcaggccg gcagtgcagt tgttgaccgc gagcgcgtgc
     4141 ggtgccttta cgcgagcggc gaacgccgcc tcgaactcgc gcgacatggg gccctggccg
     4201 gccacccagc cggattccag cacctttcgg acggcttcga gctcctcggg gccggtcgtc
     4261 ggctggccga gtgcgatctg gtctggcatc gaggtcctcc ggcgggcggg acccacgccg
     4321 atggccgggg tcctggcgag atgtccggca gggagtctac ggactgtccc ggtggccgtg
     4381 ggtcatggct cgccgccgag tcacggctgt gcggcgggca tcacctagca tcgacggccg
     4441 acccaccgcg ttccgtgtcc ccctggaggg tgtcccgcat gatcccgatc tccgtcgtcg
     4501 acctcggccc cggtgccgag gagctcgtcc aacaggtgct caagtccggg cacctcgccc
     4561 aaggagcccg tgtcgcggag ttcgaggccc ggttcgccga gctcaccggc gtcacccacg
     4621 ccgtggcagt caacaacggc acgaccgccc tggtcgctgc gctccgtgtg ctcgaacttg
     4681 agcccggcga cgaggtgatc acgaccccgt tcacgttcgt cgcgaccctc aacgcgatcg
     4741 tcgacgccgg cgcgtcggta cggttcgccg acatcgccga cgccgacttc aacatcgatc
     4801 cggccttggt cgaggcccag ctcaccgacc ggacccgcgt cctgctaccg gtgcacctct
     4861 acggccagtg cgccgatctc gatcgactcg acccgatcgt ggccgcgaac gacctccagc
     4921 tcgtcgagga cagcgcccag gcgcacggcg ccaccatcgg cagccgcgcc gccggagcca
     4981 ccggcctcgg ttgcttctcg ttctacgcga cgaagaacat caccaccggc gaagggggga
     5041 tgatcaccac cgacgacgac cggctcgccg atcggctccg gatccttcgg aaccagggca
     5101 tgcggagccg ctaccagtac gagatggccg gcaacaacta ccgactcacc gacctccagg
     5161 ccgtcctcgc gctgccacag ctcgacggct acgccacgaa cgtcgcccgg cgtcgggcca
     5221 acgccgccgc cctgtccgcc ggtctcgctg gaatcgacgg ggtcgtgacg ccgagcgagc
     5281 tgcccgggcg cggccacgtc tggcaccagt acacgatccg catcaccggc gacgcgcccg
     5341 tgagccgtga caccgtgctc gagcgcctcc aggccgacgg catcggctgc ggcgtgtact
     5401 acccgaaggt cgtgttcgac tacgactgct tcgccggcca tcccggcatc cacaccgccg
     5461 acctcccggt cgcccgccgt ctggccggcg aggtgctgtc gctgccggtg cacccggcgc
     5521 tcaccgacgc cgagctcgac cgcatcgtcg agggcgtccg cgacgccgtc agcgcctgat
     5581 ggccggcgtc cgcatcggcc tcgtcggggc cggcagcatg ggttcgttgc acgcccgcgt
     5641 cgtgtcggag tccccccgcg ccgacctcgc ctggatcgcc gacccgagcc gaccggccgg
     5701 cgaggccgcc gcgacgcggt ttggtaccac ctggctcccg catctccgga ccgacgacgt
     5761 cgatgccgtc gtcatcgccg ccgcgaccga agcccacccg aagctggcgg acgaggtgct
     5821 cgacgctggc atcccgctgc tggtcgagaa gcccctcgcc gacgacatcg tcgacacccg
     5881 acgtctggtc gagcggtccg ccgccgccgg catcccgctc atgtgcgggt tcctcgagcg
     5941 ctacaacccg gcgatccgca cggcgctcga cttcgtcgag cacccgctcc acgccacggc
     6001 catccgccat tcgccctacg tgccccggat ccgcaccggc gtcgcctccg atctcctcat
     6061 ccacgatctc gacacgatgt tgcgcctgtt cgggcagttc cccgactcgg cgtcgggcac
     6121 ggccggctac ttccaccccg acagcgaccc ccgctccgag gacgtcgccg acgccgtgct
     6181 gcgattctcg aacggctcga tcggcgccgc ctccgccagc cggatctccc aacggaaggt
     6241 ccggacgttg gtcatcggtg agctcgaccg ccagatcgag ctcgacctcg tccggcagga
     6301 catcacgatc taccggcacc tcggcaatgc tccgaccgac gaggagagcg gcctcggcta
     6361 cagccaacag accgtgatcg acattccggt catccgacac cagcgcgagc ccctcgcttc
     6421 ccagctcgac cggttcctcg acctgatcga cggcaccggc gaccccgacg aggagcgggc
     6481 gaccctgttg ccgccgcacg agctcctcgc caccatccgc gcttcgatcg acgacgccgc
     6541 cacacgtgcc tgaatcgggc ggcaccgacg gccggccgat cgaccggatc cgtcaggcga
     6601 tggacctggc ccgctcgggt caggcggcga tgaccgtcgg actgctcgcc aacggcatcg
     6661 cctcgtacgt cttcctcgcc gccagcggtc gggccctggg cccgtcccgg ttcgccgccg
     6721 tctcggtgct ctgggcggtg ctgttcctcg tcggctcggg cctcttccag ccgttcgagc
     6781 aggagctcgg ccggtcgatc gcggcgcgcc gcgcccgtgg cgacgggttc ggcgcgctcg
     6841 tccgccgagc cgccgtgctg gccatggcac tgttcgcgat cgtggcggtc ggcatcgcga
     6901 tcgcgacccg ccccatcgcc gacgcactgt tccggggcga cgaaggcttc gtcgtcgcgc
     6961 tgatcgtggg catcggcggc gtcggcgtca tgctcgtgac gcgcgggctc ctggccggct
     7021 cgggccgcta ctacggctac gcggtgctgt tcttcgccga tgccgcgacc aagtcgatcc
     7081 cggcgctggc gctcgccgtc gccggtgtca gggagccgtt ggtcttcgcc ggcgtggtgg
     7141 ccgtcagcgc gtacctcggg gccctcgtcc cgctgtcgaa gggcacgcgg ctcagcgcgc
     7201 ccggccccga gcccgcctgg ggtccactct catcgtcggt cggcttcctc ctgctcacgt
     7261 cgctgctgag ccagatcacc ctcaacatcg gcacgttggc agtcgagatc ctcgcgacca
     7321 agtcggagca tgaccgggcc ggggtcttcc tgtccggcct cgtcatcgcc cgcgtcccgc
     7381 tgttcctgtt ccaagccgtc caggccatcg tgctcccccg cctgtcccga ctggcggcct
     7441 cgggtgacct tgacgggttc cgggacaacg tccggaagct gctcgcggca atggtcggcg
     7501 ccaccgccct ggcgacggcg gtgtcggcag cggtcggacc catcgcggtg aagatcctgt
     7561 tcggggagga ctacggcctc ctcggatcgc gcgacatggg catgctgacg ttcgccagcc
     7621 tcctgatgat gtgcgcgctg accatcaacc aggcgcagat cgccctccac caccagcacc
     7681 agac
//
LOCUS       DAOSOA010000133         7679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9525_length_7679_cov_4.441238, whole genome shotgun sequence.
ACCESSION   DAOSOA010000133 DAOSOA010000000
VERSION     DAOSOA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9525_length_7679_cov_4.441238"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKY13_09530"
     CDS             complement(<1..798)
                     /locus_tag="PKY13_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOT80216.1"
                     /translation="MAPSPAQFEALFNPRGVVVAGASTHPGKFGFVSLHNILASGYEG
                     KVFATNLDGAPVLGINTVPSIDDLPDGEADLVFVCTPKGANPDLLRACARKGITAAFL
                     TSAGYGEAGEQGRVDEAELVALCDELGILLAGPNGQGVVSTPVGLCAQIVAPYPPAGS
                     IGVASQSGNFVSSFLNMATATGIGISRAVSAGNAAATTVADYLSWYSTDPATSVGLAY
                     VEGIDDGRAFFDAVRAITPTKPVVLVKGGATAGGARAASSHTGSLATD"
     gene            complement(798..1520)
                     /locus_tag="PKY13_09535"
     CDS             complement(798..1520)
                     /locus_tag="PKY13_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT80217.1"
                     /translation="MSSLTPPSRSTRTLSEAESKQFLAPYGLPIAPERVVGTAAAAAA
                     AAAEMGFPVVAKLCGDSIAHKTERGLVRLNLGTAAAVEAAAADLLGAATADDGEVGVL
                     VAPMVAGTRELIAGLSRDEQFGMTVMLGIGGILAEAIADVAIRLVPITADDATSMLDE
                     LEHQSLLGAFRGEVEVDRAAVVATLMALNDAAVANPELVSVDLNPLIINAGRPVAVDA
                     LVELTDDPTEAPTEARAPGAAR"
     gene            1602..1991
                     /locus_tag="PKY13_09540"
     CDS             1602..1991
                     /locus_tag="PKY13_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80218.1"
                     /translation="MQIVAALFTDEINLRQVAGPSTRIDLTGVQFSAVAPSPLPLEWA
                     PHLVVIIRCGAGESTTAALEVVYYRDGEQIARNVQAVEIEQNKFGYRLVRAEMEFTEY
                     GTVEAHCRMDMGPVTIVPYTLLPPPEA"
     gene            1991..>3105
                     /locus_tag="PKY13_09545"
     CDS             1991..>3105
                     /locus_tag="PKY13_09545"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOT80219.1"
                     /translation="MGPTFAAALSEHPLATQAVGEVVGQVLESLGTPPDVAMLFVTGP
                     HAGALEDIARAVRRLVNPRVLVGATAVSVLGGSQEVEETPGIVLWAARFDVDVEPVRL
                     EAIPTADNLFVAGGGVLNRNDGTLLLVADPFSFPVEQVLGHLRESAPDVAIVGGMASA
                     ARGPGGNVLVLDDELFRSGAVGVWLPPSVPISTVVSQGCRPIGQPFVVTKAERNVIYE
                     LAGQEALSRLNETIDSLSDHDKRLLANGLHIGHVIDEQRIDYGRGDFLIRGVLGADRK
                     IGAIAIGDEVPVGATVQFQVRDAESADEDLHALIDGQRARGALVFTCNGRGSNLFGTP
                     GHDAEVVSEQTGGAVAGMFCAGEFGPVGSVNFVHGFTA"
     assembly_gap    3106..3205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3206..3980
                     /locus_tag="PKY13_09550"
     CDS             <3206..3980
                     /locus_tag="PKY13_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT80220.1"
                     /translation="MRRRFLIVLTTAALFAGVAGCDAAAFNRWLVERGKPAMTEPELS
                     KVVALITAAENENTRKASFTGTLVGVDAARLGLSWRPGCPVGPADLRLITVSYWGFDN
                     AGHTGELIVHRKIALQTIRAFRELWDAKFPINRMDTAEKFASPTDFRPDGSFIETHGP
                     DLVNDTSAFFCRPTTGSSSGWSQHSYGTAIDINPVQNPYIKGATVIPENGTTARNASV
                     PGTIVKGSLPVAAMKRAGLVWGGTWSSLKDYMHFSATGR"
     gene            4155..6128
                     /gene="tkt"
                     /locus_tag="PKY13_09555"
     CDS             4155..6128
                     /gene="tkt"
                     /locus_tag="PKY13_09555"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT80221.1"
                     /translation="MGFTADQERLGINVVKALAMDGPAKANSGHQGTAMALAPLAHVL
                     FTRVMRYDATAPDWPDRDRFVLSAGHASILQYSFLYLTGYGLTLEDLEQFRQWGSATP
                     GHPEVHHTAGVEVTTGPLGQGFANAVGMAMAEQSLRARFGEAATNHHIWCIAGDGDLS
                     EGISHEAASLAGHLQLDHLTVIYDDNHVSIDGVTELALTDNAAERFRGYGWNVVEVGE
                     IAEDLDALEAALVAAKAHSGAPTIIILRSHIGFPSPTLTDSPSAHGLAFKPADIEATK
                     EVMGLPTDQSFYVPDEVVELYRAAGTAGSAARLAWEAGLDSALGERHAEWTARLGGTG
                     LPGWESALPTFEVGDAVATRVALAKALTAIVDVVPGLMAGGADLTGNTGTELKGQTAL
                     SPEHPEGRQIHFGVREHAMGATMNGMALHGGVLPVGGTFLVFSDYMRPSVRLAALMGT
                     KVIFAWSHDSVGVGEDGPTHQPVEHVAALRAIPGLDVMRPADANETVHAVRLAVEGNA
                     PMALIMSRQNLPVLEGTSFEGVANGGYVLREAEGAQLNLVATGSEVSVILAAADRLAA
                     DGIVARVVSLPSWERFEAAGRSYQASVLPSDVPTLAVEAQVTFGWHRWADDVIGIDRF
                     GASAPGDLVLDKLGINVDNVVARATALVQDEQS"
     gene            6142..7239
                     /gene="tal"
                     /locus_tag="PKY13_09560"
     CDS             6142..7239
                     /gene="tal"
                     /locus_tag="PKY13_09560"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOT80222.1"
                     /translation="MGRLHDLYEFEGQSPWLDNLKRAWLESGEIQQWIDRGVRGITSN
                     PSIFQKAIETGEEYTAQFSELIGSGSSVEDAYWKLVVQDIEGALALLRPVYDESDGID
                     GYVSVEVAPSLAHDREGTEEAARVLDDLIDEPNLLVKIPATEAGVGAIRAMIGESRSV
                     NVTLIFSLERYAAVMEAYIAGLEDALADGAVDLSAIASVASFFISRVDVEVDKRLEAV
                     GTPEALALRGKAAYVQGQLAYEMFTQAFSGPRWEALEAKGARVQRPLWASTSTKNPSY
                     PDTLYLDTLIGPDTVNTVPDATLVAFEDHGTVARTVDADLPGAHAVWDAVGALVDLDD
                     VAATLETEGVDAFMKSFDDLLEVLSKKAAEL"
     gene            complement(7181..>7679)
                     /locus_tag="PKY13_09565"
     CDS             complement(7181..>7679)
                     /locus_tag="PKY13_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80223.1"
                     /translation="PYVAGFWYTENSFKRIPRWAFAAASAACAAGAWWYDGIRPGAVN
                     ASGIKHLLVGGAWLAASFAAAPQLRRVADRFRTVIDRISRRTFTIFLWGWTTSVVAWD
                     RAGSITDGVWQRRSLYWVFALALLAAAVAAFGWVEDVAAGRTRRSTALRPSWTAPRGG
                     RRSSS"
BASE COUNT         1155 a         2711 c         2618 g         1095 t
ORIGIN      
        1 gtcggtcgcg agcgaaccgg tgtgactcga cgcggcgcgg gccccgcccg ccgtggcgcc
       61 gcccttgacg agcaccaccg gtttggtggg cgtgatcgct cgcacggcgt cgaagaacgc
      121 ccggccgtcg tcgatgccct cgacgtacgc gagcccgacc gacgtggccg ggtcggtcga
      181 gtaccaactc aggtagtcgg ccacggtcgt ggcggcggcg ttgccggcgg acacggcccg
      241 actgatgccg atgcccgtcg ccgtcgccat gttgaggaac gacgagacga agttgcccga
      301 ctggctggcg acgccgatcg agcccgccgg cgggtacggc gccacgatct gcgcgcacag
      361 gccgaccggt gtcgacacga caccctggcc gttggggccg gccaacagga tgccgagctc
      421 gtcgcacaag gcgaccagct cggcttcgtc cactcggccc tgctcccccg cctcgccgta
      481 gccggccgac gtcaggaatg ccgccgtgat gcccttgcgg gcgcaggcgc gcagcaggtc
      541 ggggttggct cccttggggg tgcacacgaa gacgaggtcg gcctcgccgt cggggaggtc
      601 gtcgatgctc ggaaccgtgt tgatgcccag gaccggggcg ccgtcgaggt tggtggcgaa
      661 caccttgccc tcgtagccac tcgcgaggat gttgtggagc gacacgaagc cgaacttgcc
      721 ggggtgggtc gacgctcccg cgacgacgac accgcgaggg ttgaacagcg cctcgaactg
      781 ggcgggcgac ggtgccatca gcgggctgca cccggcgccc gggcctcggt gggggcctcc
      841 gtcgggtcgt cggtcagctc gaccaacgcg tcgaccgcca cggggcggcc ggcgttgatg
      901 atgagcgggt tgaggtcgac cgagacgagt tcggggttgg cgaccgctgc gtcgttgagc
      961 gccatgagcg ttgcgacgac ggcggcacga tcgacctcga cctcgccccg gaaggcgccg
     1021 agcaacgact ggtgctccag ttcgtcgagc atcgaggtgg cgtcgtcggc ggtgatgggc
     1081 acgagccgga tcgccacgtc ggcgatcgcc tcggccagga tgccgccgat gccgagcatc
     1141 accgtcatgc cgaactgctc gtcgcggctg agcccggcga tgagctcgcg ggtgccggcg
     1201 accatcggag cgaccagcac tcccacctcg ccgtcgtcgg cggtcgccgc tccgagcagg
     1261 tcggcggcgg cggcctcgac ggcggcggcg gtgccgaggt tgaggcggac gaggccgcgt
     1321 tcggtcttgt gggcgatcga gtcgccgcag agcttcgcga cgacggggaa acccatctcg
     1381 gcggcggcag cggcggcagc ggcggcggtg ccgacgaccc gttcgggggc gatgggcaga
     1441 ccgtaggggg cgaggaactg cttcgattcg gcctcggaca acgtacgcgt gctgcgggac
     1501 ggtggtgtga gcgacgacat gatcggacga gcctacgcgc ggcccgcacc ccccgacgct
     1561 gaccgcaccc caccggttcg ggcagttggt accgtgctcg catgcagatc gtcgcggccc
     1621 tgttcaccga tgagatcaac ctccgccagg tggcgggccc gtcgacgcgg atcgacctga
     1681 cgggcgtcca gttctccgcc gtcgccccgt cgccgctccc actcgagtgg gcgccgcacc
     1741 tggtcgtcat catccgctgt ggcgccggcg agtcgacgac cgcggcgctc gaggtcgtgt
     1801 actaccgcga cggcgagcag atcgcccgca atgtgcaggc cgtcgagatc gagcagaaca
     1861 agttcggcta ccgcctcgta cgggccgaga tggagttcac cgagtacggg acggtcgagg
     1921 cgcattgccg catggacatg ggcccggtca cgatcgtgcc ctacacgctg ctccccccgc
     1981 cggaggcctg atgggcccga ccttcgccgc ggcgctcagc gagcaccccc tcgccacgca
     2041 ggccgtcggc gaggtcgtcg ggcaggtact cgagtcgctc ggcaccccgc ccgatgtcgc
     2101 catgctgttc gtgaccggcc cccatgccgg cgcattggag gacatcgccc gggcggtgcg
     2161 ccgactggtc aacccacggg tgctcgtcgg ggccaccgcc gtgtcggtcc tcggagggtc
     2221 ccaggaggtc gaggagacgc cggggatcgt gttgtgggcg gcgcggttcg acgtcgacgt
     2281 cgagccggtc cgcctcgagg ccatcccaac cgccgacaat ctcttcgtgg ccggcggcgg
     2341 tgtcctcaac cgaaacgacg gcacgttgct gctggtcgcc gacccgttca gcttccccgt
     2401 cgagcaggtg ctcggtcatc tccgcgaatc ggcaccggac gtcgcgatcg tcgggggcat
     2461 ggcgtccgcg gcgcggggtc ccggcggcaa cgtcctggtg ctcgacgacg agctgttccg
     2521 cagcggtgcg gtcggggtgt ggctcccacc gtcggtgccg atcagcacgg tggtgtcgca
     2581 gggttgccgg ccgatcggcc aaccgttcgt ggtgaccaag gcggaacgca acgtcatcta
     2641 cgagctcgcc ggccaggagg cgctcagccg tctcaacgag acgatcgact cgctcagcga
     2701 ccacgacaag cggctgctgg cgaacggcct ccacatcggc cacgtgatcg acgagcagcg
     2761 gatcgactac ggccgcggcg acttcctcat ccggggcgtc ctcggcgccg accgcaagat
     2821 cggggcgatc gccatcggcg acgaggtgcc ggtgggagcg acggtgcagt tccaggtccg
     2881 cgacgccgaa tccgccgacg aggacctcca cgccctcatc gacggccaac gagcccgggg
     2941 cgccctcgtg ttcacgtgca acggccgggg ctcgaacctg ttcggcacac ccggccacga
     3001 cgccgaggtc gtcagtgaac agaccggcgg cgcggtcgcc ggcatgttct gcgccgggga
     3061 gttcggtccg gtcggcagcg tcaacttcgt gcacggcttc accgcnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnncatgc gccgccgctt cctgatcgtc ctcaccaccg
     3241 ccgcactgtt cgccggtgtc gccggttgcg acgctgcagc gttcaaccgc tggctcgtcg
     3301 agcggggcaa gccggccatg accgagcccg aactgtcgaa ggtcgtggcc ctcatcacgg
     3361 ctgcggagaa cgagaacact cgcaaggcgt cgttcaccgg aaccctcgtc ggcgtcgacg
     3421 ccgcacggct cgggttgtcg tggcgcccgg ggtgtcccgt cggcccggcg gacctgcggc
     3481 tgatcaccgt gagctactgg ggcttcgaca acgccgggca caccggcgaa ctgatcgtcc
     3541 atcgcaagat cgccctgcag acgatccggg cgttccggga gctgtgggac gcgaagttcc
     3601 cgatcaaccg catggacacg gcggagaagt tcgcgagccc aaccgatttc cgccccgacg
     3661 gaagcttcat cgagacccac gggcccgatc tggtcaacga cacgtccgcc ttcttctgcc
     3721 ggccgacgac gggctcgtcg agtggctggt ctcagcactc ctacggcacg gccatcgaca
     3781 tcaaccccgt ccagaacccc tacatcaagg gtgcgacggt catccccgag aacggaacga
     3841 cggcacgcaa cgcttcggtg ccgggcacga tcgtgaaggg ctcgctgccg gtggccgcca
     3901 tgaagcgcgc cggtctggtc tggggcggca cgtggtcatc gctgaaggac tacatgcact
     3961 tcagcgcgac cggccgctga cgccgactca cgagtggtgc gcgcgttctg ggcgcgccag
     4021 cgggtcacat ccgacccggc ggcgcgcccg gaagctgcga cccacccgtt cgggcccgcc
     4081 acggcgagcg attagggttg cctcgcacgg cgcgactcat cccccgtcgg cacttctacg
     4141 agaaggggaa ccacgtgggc ttcaccgcgg accaggaacg gctcggcatc aacgtcgtca
     4201 aagcactcgc gatggacgga ccggcgaagg ccaactccgg ccaccagggc acggcgatgg
     4261 cgttggcacc actcgcccac gtcctgttca cgcgggtcat gcgctacgac gccaccgccc
     4321 ccgactggcc cgaccgtgac cgcttcgtgc tctcggccgg ccacgcatcg atcctgcagt
     4381 actcattcct gtacctcacc gggtacggcc tgaccctcga ggacctcgag cagttccgcc
     4441 agtggggttc agcaacgccc ggccaccccg aggtgcacca caccgccggc gtcgaggtca
     4501 ccaccggacc cctgggccag ggcttcgcca acgccgtcgg catggcgatg gccgagcagt
     4561 cgctgcgcgc ccggttcggc gaggccgcca ccaaccacca catctggtgc atcgccggcg
     4621 acggcgacct gtcggagggc atcagccacg aggccgcgtc gctcgccggt cacctgcagc
     4681 tcgaccacct cacggtgatc tacgacgaca accacgtgtc gatcgacggc gtcaccgagc
     4741 tcgcgctcac cgacaacgcc gccgagcggt tccgtggcta tggctggaac gtcgtcgagg
     4801 tcggcgagat cgccgaggac ctcgacgcgc tcgaggccgc cctcgtcgcg gcgaaggccc
     4861 acagcggggc gccgacgatc atcatcctcc gtagccacat cgggttcccg tcgccgacgc
     4921 tgaccgacag cccctcggcg cacggcctgg ccttcaagcc cgccgacatc gaggcgacca
     4981 aagaggtcat gggcctgccg accgaccagt cgttctatgt gcccgacgag gtcgtcgagc
     5041 tgtaccgcgc cgccggcacc gccggctcgg cggcccgcct cgcctgggaa gccggcctcg
     5101 acagcgccct cggcgaacgg cacgccgagt ggaccgcccg cctcggtggc accggcctcc
     5161 ccggctggga atcggccctg cccaccttcg aggtcggcga cgcggtcgcc acccgcgtcg
     5221 cactggccaa ggcactcacc gcgatcgtcg acgtggtgcc cggcctcatg gccggcggcg
     5281 ccgacctcac cggcaacacc ggtaccgagc tcaagggcca gaccgccctc agccccgagc
     5341 accccgaagg tcgccagatc cacttcgggg tccgcgagca cgccatgggc gcgacgatga
     5401 acggcatggc gctgcacggc ggcgtcctgc ccgtcggcgg cacgttcctc gtgttcagcg
     5461 actacatgcg cccgtcggtg cgcctcgccg cactcatggg cacgaaggtg atcttcgcct
     5521 ggtcgcacga ctcggtcggt gtgggcgagg acggccccac ccaccagccc gtcgagcacg
     5581 tcgccgccct tcgggccatc cccggcctcg acgtcatgcg tccggccgac gccaacgaga
     5641 cggtgcacgc cgtccgcctc gccgtggagg gcaacgcccc gatggcgctc atcatgagcc
     5701 gccagaacct gccggtgctc gagggcacct cgttcgaggg cgtcgccaac ggtggctacg
     5761 tgctccgtga ggccgagggt gcacagctca acctggtcgc cacgggctcg gaggtctcgg
     5821 tcatcctggc cgctgccgac cgcctcgccg ccgacggcat cgtggcccgg gtggtctcgc
     5881 tgcccagttg ggaacggttc gaggcggcag ggcgctccta ccaggcctcg gtcctgccct
     5941 ccgacgtgcc gaccctcgcc gtcgaggcgc aggtcacgtt cgggtggcac cgctgggccg
     6001 acgacgtgat cggcatcgac cgcttcggtg catcggcgcc gggggatctg gtgctcgaca
     6061 agttgggtat caacgtggac aacgtcgtcg cccgggccac cgccctggtt caggacgagc
     6121 agagctgaac ggagacagat gatgggtcgt ctacacgatc tctacgagtt cgagggccag
     6181 agcccgtggc tcgacaacct caagcgtgcg tggctcgagt ccggggagat ccagcagtgg
     6241 atcgatcgcg gcgtccgggg catcacctcc aacccgtcga tcttccagaa ggcgatcgag
     6301 accggcgagg agtacaccgc gcagttctcg gagctgatcg gctcgggctc ctcggtcgag
     6361 gacgcgtact ggaagctggt cgtgcaggac atcgaagggg ccttggcgct gctgcgcccc
     6421 gtctacgacg agtccgacgg catcgacggc tacgtctcgg tcgaggtggc accgtcgctc
     6481 gcccatgacc gcgagggcac cgaggaagcg gcccgcgttc tcgacgatct catcgacgag
     6541 ccgaatctgc tggtcaagat ccccgccacc gaggccgggg tcggggccat ccgggcgatg
     6601 atcggcgaga gccggtcggt caacgtgacg ctgatcttct ccctcgagcg ctacgccgcg
     6661 gtcatggagg cgtacatcgc cggcctcgag gacgccctcg ccgatggtgc agtcgacctg
     6721 tccgccatcg cgtcggtggc ctcgttcttc atcagccgcg tcgacgtcga ggtcgacaag
     6781 cgtctcgagg cggtcggcac gcccgaggcg ctcgcgctgc gcggcaaggc tgcctacgtc
     6841 cagggccagc tggcatacga gatgttcacc caggcgttct ccggtccgcg ctgggaggcg
     6901 ctcgaggcga agggggcccg ggtgcagcgg ccgctgtggg cgtcgacgtc gacgaagaac
     6961 ccgtcctacc ccgacacgct ctacctcgac acgctcatcg gccccgacac ggtcaacacg
     7021 gtgcccgacg ccaccctcgt ggcgttcgag gaccatggca ccgtcgctcg caccgtcgac
     7081 gccgacctgc ccggagccca tgccgtgtgg gacgcggtcg gagccctggt cgatctcgac
     7141 gacgtcgctg cgaccctcga aaccgagggc gtcgacgcgt tcatgaagag cttcgacgac
     7201 ctcctcgagg tgctgtccaa gaaggccgca gagctgtaga tcgccgcgtt cgcccggccg
     7261 cgacgtcctc gacccacccg aacgcggcga ccgccgcggc caggagtgcg agggcgaaca
     7321 cccagtacag cgagcgtcgc tgccagacgc cgtcggtgat ggacccggcg cggtcccagg
     7381 cgacgacgct cgtcgtccat ccccagagga agatcgtgaa cgtgcggcga ctgatccggt
     7441 cgatgacggt gcggaaccgg tcggccacac ggcggagctg aggcgctgct gcgaacgagg
     7501 ccgcgagcca cgctcccccg acgaggaggt gcttgatgcc cgacgcgttc acggctccgg
     7561 gccggatccc gtcgtaccac cacgccccgg cggcacacgc tgccgacgct gccgcgaacg
     7621 cccaccgggg gatccgcttg aacgagttct cggtgtacca gaagccggcg acgtagggg
//
LOCUS       DAOSOA010000134         7665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9547_length_7665_cov_6.071748, whole genome shotgun sequence.
ACCESSION   DAOSOA010000134 DAOSOA010000000
VERSION     DAOSOA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9547_length_7665_cov_6.071748"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKY13_09570"
     CDS             <1..671
                     /locus_tag="PKY13_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT80224.1"
                     /translation="IAGAGSTPWYWHLVEPLLREYGHDTIAPHLPVDDVAATFEDYAQ
                     VVIDTIGDRRDRDDIVLVAQSLGGYTAPLVADRLAARLIVMVAAMVPVPGESAGEYWA
                     ATGAEQARIDLARADGRNPDDMDAMFLHDVPEAVVIDGQAHLRNQSGTPFAAPFPLAA
                     WPDVPTRFLLCSDDRFFPADLLRRIVPERLGITPDEMEGGHLPALAHPDELARRLHDY
                     ADGR"
     gene            793..2661
                     /locus_tag="PKY13_09575"
     CDS             793..2661
                     /locus_tag="PKY13_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80225.1"
                     /translation="MNSPIPSPPSTVGSRLKYRFDNALSRGPSVVIGWLGFITIGIVI
                     MVGLLVALAQVGGVSGGKPVGIIEGVWQALTRVVDAGTFVGDTTWPARLIGLLITLAG
                     IFIAGSLIGLIASAVDGQIEQLRKGRSAVLENDHVLILGWSERVPAIVAELVIANESE
                     KRAAVIVLANEEKDLMEDTLRDAIEDPQTTRIVCRNGDPAAPKDLIRANLANSRAVIC
                     VNGSEGDAGVVKAVLAVNTLDEHFAGRHLVAELEDPELAMTLRTIMGDRIVPVNSDRL
                     IAELTAQACRQRGLSQVFRDLLDYDGDEIYFGAFPELEHHTFGEARLAFEQCSPIGIQ
                     PVGGRVILNPPGETVLGPGDELVAIARDDSEFTISSVPTVSGGGHRGAPGVSEQRNLL
                     VVGWSRLAPTVIRELDEFLPDGAKVTVLVDPDLADPDEVKRVVATARTEITVDVHSGG
                     PEQILERDLSDVEKAVVLGYRDSLDVGDADARSLLTVLALRHCKPVGLQIVAEVLEAR
                     SVPIAQASGADDFVVSGELTSLMMAQLSGRHELFDVFNDLFDAGGATVQIHAMHRYEI
                     PGGASYHDIVEILGSAGVSAIGYRQSGSGDVVLNPSRSVVPNLGPSDELIVIGSVG"
     gene            complement(2726..3619)
                     /locus_tag="PKY13_09580"
     CDS             complement(2726..3619)
                     /locus_tag="PKY13_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT80226.1"
                     /translation="MELSTVRVHGHEVAYRTAGEGPVVLLVHGMAGSSSTWKPVIPLL
                     AAHATVVAPDLPGHGRSAKPRGDYSLGASASFLRDLITTLGHDRVTIVGQSLGGGVAM
                     QFCYQYPERVERLVLVGSGGLGEEVMSILKVLAAPGVDLLMTPAFLPFFRDAGNAVGR
                     ALAKIGLRPSPQAFEMWRAYASLTDGATRSAFVQTLRAVVDTHGQRVSAHDKLYLSAR
                     VPSMIVWGDQDPVIPVSHAYAAHEAMPASRLEIFEGCGHFPHFEAPAEFAEILLDFMA
                     TTEPATITADDLKLMLAPPAA"
     gene            complement(3671..3997)
                     /locus_tag="PKY13_09585"
     CDS             complement(3671..3997)
                     /locus_tag="PKY13_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80227.1"
                     /translation="MDDQEKEDIGKMAGLGGGVLSGAALGSAIPIPVVGTFVGAVVGG
                     VVGSQVGQSLGKAVYDGAEAVGKAVVEGAGGIGKAVANGAEAFVDTIKGSSSEKPSGD
                     DEGNDL"
     gene            4146..4532
                     /locus_tag="PKY13_09590"
     CDS             4146..4532
                     /locus_tag="PKY13_09590"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT80228.1"
                     /translation="MPSFPVTIRPLSLRVLEGALVVEAGDDLDGIDDSVIGVVRAPDS
                     VTIVRRSAPGSPPAAAWRALYADAGHALDLPGVLVGVLAPLANAGIEVYVLSTIDRDL
                     VLVPEDLVADAADALRLAGHAVAPID"
     gene            4585..5547
                     /locus_tag="PKY13_09595"
     CDS             4585..5547
                     /locus_tag="PKY13_09595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT80229.1"
                     /translation="MTKLGYQIPNFTYPGVAADQIFDNLVTQAKTAEAAGFDRIFLMD
                     HFYQLPGIGDPDDYMLECYTTLAALAQHTDKVRLSALVTGNTYRNPAVLAKIITALDH
                     VSHGRASLGIGSGWFELEHDAYGIPFNTFSERFEKLEEALNIILPMLRGERPTLHGEH
                     YHVTEAINSPAPVSKIPVMIGGNGEKKTLRMVAQYADEANFTCTPEEVPRKMEALSTH
                     CERLGRDRSEIKVTKLVMMVIGNTHDEAVASADEFIANHGWPPEVVELLKPRMTLGDP
                     DEVGAEIVKLKELGLDGITMNLVAGAHRPEMIELAAKTSLKALG"
     gene            complement(5620..6294)
                     /locus_tag="PKY13_09600"
     CDS             complement(5620..6294)
                     /locus_tag="PKY13_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HOT80230.1"
                     /translation="MTPSVSTRPGIRARAAVVAEFRSGRTALTTLRSEPPLTLRPTAD
                     ALYVIGTAAGPLGGDELTFDIAVGDGATLTLRTVAASIVLPGPGGGRSSTVTNVVVGR
                     GATLVYEPEPVIAVAGCDHVATTIIQLAADATLVWNEHLVLGRHGEESGSILQRLRVD
                     RAGAPLYRNDLALGPVHPGWDTPAGIGEARRVDTTLTVGAAAPVRPGATVMPLAPDAT
                     LTLALR"
     gene            complement(6311..6982)
                     /gene="ureG"
                     /locus_tag="PKY13_09605"
     CDS             complement(6311..6982)
                     /gene="ureG"
                     /locus_tag="PKY13_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HOT80231.1"
                     /translation="MGAHHGPDDPHRHTHAGDALRIGIGGPVGSGKTELVGALCRAFA
                     EELEMVVVTNDVFTMEDAAILERKAVIPVERIAGVQTGCCPHTAIRDDISENLDVVER
                     LEAKFKPDLVLLESGGDNLTATFSKALVDLQLFVIDVAGGDKVPRKGGPGITTSDLLV
                     INKTDLAPLVHADLDVMRRDADEKRGDLPTAFISLTEDPLATDIATWIRQQLGEWDPG
                     AHHEH"
     gene            complement(7000..>7665)
                     /locus_tag="PKY13_09610"
     CDS             complement(7000..>7665)
                     /locus_tag="PKY13_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HOT80232.1"
                     /translation="RFPAGGHVHSAGIESAVADGRVTGQRSLEDYLRGRLTTVGLTDA
                     ALVAASVIRLDAEQSDGSDLLGSVIAELDAEVEARIAPPPLRSASRKLGRQLTRVAAR
                     CWPDPLFASLSAAHLEGVHQPVALGAAAVVSGASPVGAATVSVHHALNTPAQAAVRLL
                     GLDPFAAVAIAVSLAPEADAIVAAAVAAAHGPLADLPARTGPRCEIAAVDHETWDLRL
                     FAT"
BASE COUNT         1228 a         2739 c         2555 g         1143 t
ORIGIN      
        1 tgatcgcggg cgcggggtcg accccgtggt actggcacct cgtcgagcca ctcctgcgcg
       61 agtacggcca cgacacgatc gcgccgcacc tccccgtgga cgatgtcgca gcgacgttcg
      121 aggactacgc gcaggtggtg atcgacacca tcggcgatcg gcgcgaccgt gacgacatcg
      181 tgctcgtcgc ccagtcgttg ggcgggtaca ccgcacccct ggtcgccgac cggctcgccg
      241 cccgactgat cgtgatggtc gctgccatgg tgccggttcc gggtgagtca gcgggcgagt
      301 actgggcggc caccggcgcc gagcaggcac gcatcgacct ggcgagagcc gacggccgca
      361 accccgacga catggacgcc atgttcctgc acgacgtgcc cgaggccgtc gttatcgacg
      421 gccaggcgca ccttcgcaac cagtccggca cgccgttcgc ggcaccgttc cccctggccg
      481 cctggcccga cgtgccgacg cggttcctgc tgtgcagcga cgaccggttc ttccccgccg
      541 acctgctccg ccgcatcgtg cccgagcggc tcggcatcac ccctgacgag atggaggggg
      601 gacacctccc ggcgctcgcc catcccgacg agctcgcccg gcgcttgcac gactacgccg
      661 atggccggta gcccgccgat cttcggaccg cgacggtcgc tcacgcaaga tccgggccga
      721 agccgcgtta cgactgctgc agaaccattc agtgttgaat cgattcggga gatcggtcgc
      781 tagcgtgacc gggtgaactc acccattcct tccccgccgt cgaccgtcgg ctcgcggctg
      841 aagtaccgct tcgacaacgc cctgtcccga ggaccctccg tcgtcatcgg ctggctcggg
      901 ttcatcacca tcggcatcgt catcatggtg ggcctcctcg tcgcgctggc gcaggtcggc
      961 ggcgtcagcg gcggcaagcc cgtcgggatc atcgaagggg tgtggcaggc gctgacgcgc
     1021 gtcgtcgatg cgggcacgtt cgtcggcgac acgacctggc cggcccggct gatcggcctc
     1081 ctcatcaccc tcgcgggcat cttcatcgcc ggcagcctca tcggtctgat cgcctctgcc
     1141 gtcgacggcc agatcgagca gctccgcaag ggccggtccg ccgtgctgga gaacgaccac
     1201 gtgctcatcc tcggatggtc cgagcgcgtg cccgccatcg tggccgagct ggtcatcgcc
     1261 aacgagagcg agaagcgcgc cgcagtcatc gtcctcgcca acgaggagaa ggacctgatg
     1321 gaggacacgc tccgcgacgc catcgaggac cctcagacga cccgcatcgt ctgccgcaac
     1381 ggcgacccgg ccgcaccgaa ggacctcatc cgcgccaacc tggcgaacag tcgcgccgtc
     1441 atctgtgtga acggcagcga gggcgacgcc ggggtcgtga aggccgtgct cgccgtgaac
     1501 accctcgacg agcacttcgc gggacggcac ctcgtggcgg agctcgagga tccggaactg
     1561 gcgatgaccc tccgcacgat catgggcgac cgtatcgtgc ccgtgaactc ggatcgcctc
     1621 atcgccgagc tgacggcgca ggcgtgccgc cagcgaggcc tgtcgcaggt cttccgcgac
     1681 ctgctcgact acgacggcga cgagatctac ttcggagcgt tccccgaact cgagcaccac
     1741 accttcggcg aggcccgcct cgcgttcgaa cagtgctcac cgatcggcat ccaacccgtc
     1801 ggtggacggg tcatcctgaa cccacccggt gagaccgtgc tcggccccgg cgacgaactc
     1861 gtcgccatcg cccgcgacga cagcgagttc accatctcgt cggtgccgac cgtttccggt
     1921 ggcgggcacc gcggcgcacc cggcgtgagc gaacagcgca acctgctggt cgtcggctgg
     1981 agccggctcg ccccgaccgt cattcgcgag ctcgacgagt tcctccccga cggcgccaag
     2041 gtgaccgtcc tggtcgaccc ggacctcgcc gaccccgacg aagtcaagcg ggtcgtcgcc
     2101 accgcccgca ccgagatcac cgtcgacgtc cactcgggcg gcccggaaca gatcctcgaa
     2161 cgcgacctgt ccgacgtcga gaaggccgtc gtgctcgggt accgcgacag cctcgacgtc
     2221 ggcgatgccg acgcccgctc gctcctcacc gtcttggcgc tgcggcactg caagcccgtc
     2281 ggcctgcaga tcgtcgccga ggtgctcgag gcgcgttcgg ttccgatcgc ccaggcgtcg
     2341 ggcgccgacg acttcgtcgt cagcggcgag ctgacatcgc tgatgatggc gcagctgtcg
     2401 ggccgccacg agctgttcga cgtgttcaac gacctgttcg acgcaggcgg tgccaccgtc
     2461 cagatccacg cgatgcaccg ctacgagatc cccggcggcg cgagctacca cgacatcgtc
     2521 gagatcctcg ggtccgccgg tgtgtccgcg atcggctacc gccagagcgg cagcggcgac
     2581 gtggtcctca acccctcgcg ctcggtggtg ccgaacctcg gaccgagcga cgaactgatc
     2641 gtgatcggct cggtgggctg accttctggc ggcgaaactt cggcctcacc cgttcgtatg
     2701 tgaacggctg agcctcgagt tcgactcaag ctgccggtgg ggccagcatc aacttgaggt
     2761 cgtcggccgt gatggtggcg ggttcggtcg tcgccatgaa gtcgagcagg atctcggcga
     2821 actcggccgg ggcttcgaag tgcgggaagt gtccgcaccc ctcgaagatc tcgagacggc
     2881 tggccggcat ggcctcgtgc gccgcgtacg cgtgcgagac cgggatcacc gggtcctggt
     2941 cgccccacac gatcatcgac ggcacccggg ccgagaggta cagcttgtcg tgagcgctga
     3001 cccgctgacc gtgggtgtcg accacggcac gcaacgtctg cacgaacgcc gatcgggtcg
     3061 caccgtcggt caacgacgcg tacgcccgcc acatctcgaa ggcctgcggc gacggtcgca
     3121 acccgatctt ggcgagggcc cggcccaccg cgttgccggc atcgcggaag aacgggagga
     3181 acgccggggt catcaacagg tcgacgcccg gcgcggcgag gaccttgagg atcgacatga
     3241 cctcctcgcc gaggccgccc gagccgacca gcacgagccg ctcgacgcgc tcggggtact
     3301 ggtaacagaa ctgcatggcg acgccaccgc cgagtgactg gccgacgatc gtcacgcggt
     3361 cgtggccgag cgtcgtgatc aggtcgcgca ggaacgacgc cgatgcgccg agcgagtagt
     3421 cgccgcgggg cttcgcggaa cggccgtgac cgggaaggtc gggcgcaacg accgtggcgt
     3481 gggcggcgag gagcgggatg accggcttcc acgtcgacga gctacccgcc atgccgtgga
     3541 cgagcaggac cactgggccc tcgcccgcag tccgataggc gacctcatga ccgtgcaccc
     3601 gtaccgttga cagttccacg acgacctccg atgatcggac gctcgagcgt accccttcgg
     3661 tcgggttcgg ctacaggtcg ttgccctcgt cgtcgccgga cggcttctcg gaggacgatc
     3721 ccttgatggt gtcgacgaac gcctcggcac cgttggccac agcctttccg atcccaccgg
     3781 ctccctcgac gacggccttg ccgaccgcct cggcgccgtc gtagacggcc ttgccgagcg
     3841 actggccgac ctggctgccg acgacaccac cgaccaccgc accgacgaac gtgccgacca
     3901 cggggatcgg gatggcactc ccgagagccg cccccgacag cacaccgccg ccgagcccgg
     3961 ccatcttgcc gatgtcttcc ttctcctggt cgtccatctc cgccaccttt cgatcggact
     4021 gcgccgttgc gccggacggc atcacgagcg acgccggccg tgaccgaacg gtaacgcagt
     4081 tccgtcgccc gggcgcctcg ctcacgaccg cggaacccct cgggcgctca gccaaactga
     4141 gggtcatgcc atcctttccc gtcacgatcc gtccgctctc gttgcgagtg ctcgaaggtg
     4201 cgctcgtcgt ggaggcgggt gacgacctcg acggcatcga cgactcggtc atcggcgtcg
     4261 tccgcgcgcc ggacagcgtc acgatcgtgc gtcgatcagc gcccggctcg ccaccggctg
     4321 cggcgtggcg ggccctctac gccgacgcgg gacacgcgct cgaccttccc ggcgtgctcg
     4381 tcggtgtgct cgctcccctc gcgaatgcgg gcatcgaggt gtacgtactc tcgacgatcg
     4441 accgggacct ggtcctcgtg cccgaggatc ttgtggcgga cgccgccgac gcgctgcgcc
     4501 tcgccggtca cgccgtcgca ccgatcgatt gacgactgcg ggtcgcgggc gcggccatcc
     4561 tggcgcgtgc ataaggtgac cgccatgacc aagctcggct accagatccc caacttcacc
     4621 tatcccggcg tggccgcgga ccagatcttc gacaacctcg tcacgcaggc gaagacggcc
     4681 gaagcggccg ggttcgaccg tatcttcctg atggaccact tctaccaact gccgggcatc
     4741 ggcgatcccg acgactacat gctcgagtgc tacacgaccc tcgcggcgct cgcccagcac
     4801 accgacaagg tccgcctctc ggcactcgtc accggcaaca cctaccgcaa tcctgcagtg
     4861 ctggcgaaga tcatcaccgc cctcgaccac gtgtcgcatg ggcgtgccag cctcggtatc
     4921 ggctcggggt ggttcgagct cgagcacgac gcctacggca tcccgttcaa cacgttctcc
     4981 gagcggttcg agaagctcga ggaggcactc aacatcatcc tgccgatgct gcgcggcgaa
     5041 cggcccacgc tgcacggcga gcactaccac gtgaccgagg ccatcaactc cccggcgccg
     5101 gtgtcgaaga tccccgtcat gatcggcggc aacggcgaga agaagacgct ccgcatggtg
     5161 gcgcagtacg cggacgaggc gaacttcacg tgcacccccg aggaggtgcc tcgcaagatg
     5221 gaagcccttt cgacgcactg cgaacggctg ggccgtgatc gctccgagat caaggtcacc
     5281 aagctcgtga tgatggtgat cggcaacacc cacgacgaag cagtggcgtc agcggacgag
     5341 ttcatcgcca accacggctg gccgccggag gtcgtcgaac tgctcaagcc ccgcatgacc
     5401 ctcggagacc ccgacgaggt cggcgccgag atcgtcaagc tcaaggagct cggcctcgac
     5461 ggcatcacca tgaacctggt cgccggcgcg caccggcccg agatgatcga gctcgccgca
     5521 aagacctccc tgaaggcgct cggctagccg caatcaccgg ggttttgtac tgcgaaacac
     5581 gccatgtgtg gcgcgtttcg cagtgcaaaa ccccgtgggt cagcggaggg cgagcgtgag
     5641 ggtggcgtcg ggggcgaggg gcatgacggt ggcgcccggc cgaaccggag cggccgcccc
     5701 gaccgtgagc gtcgtatcga cgcggcgggc ctcgccgatt ccggcgggcg tgtcccaccc
     5761 cgggtggacc ggaccgagcg cgagatcatt gcggtagagc ggcgcaccgg ctcggtcgac
     5821 gcggagacgc tgcaggatcg agcccgactc ctcaccgtgg cgcccgagca cgaggtgctc
     5881 gttccacacg agcgtggcgt cggcggcgag ctggatgatc gtcgtggcca cgtggtcgca
     5941 cccggcgacg gcgatgacgg gctccggctc gtagaccagg gtcgccccgc ggccgaccac
     6001 cacgttggtg acggtgctcg accgaccgcc accgggccct ggaaggacga tcgaggccgc
     6061 caccgtccga agcgtgagcg ttgccccgtc gccgacggcg atgtcgaacg tcagctcgtc
     6121 tcccccgagg ggaccggcgg cggtgccgat cacgtacagc gcgtcggcgg tcggacgcaa
     6181 cgtcaggggc ggttcggaac ggagcgtcgt gagcgcggtc cgcccacttc ggaattccgc
     6241 gacgaccgcg gcccgggcgc gaatgccggg tcgcgtcgag accgacgggg tcacggaggg
     6301 ccgatagcgc tcagtgctcg tggtgtgcac ccgggtccca ctcccccagc tgctggcgga
     6361 tccacgtggc gatgtcggtc gcgagcggat cctcggtgag cgagatgaac gccgtgggca
     6421 gatcgccccg cttctcgtcg gcatcgcggc gcatcacatc gagatcggcg tggaccagcg
     6481 gggccaggtc ggtcttgttg atcacgagga gatccgacgt cgtgatgccc gggcctccct
     6541 tgcgcggcac cttgtcgcca ccggcgacgt cgatcacgaa caactgcaga tcgaccagcg
     6601 ccttgctgaa cgtggcggtg aggttgtccc caccgctctc cagcaggacg aggtccggct
     6661 tgaacttcgc ctccaaccgc tcgaccacat cgaggttctc gctgatgtcg tcacggatcg
     6721 cagtgtgcgg gcagcacccg gtctgcactc cggcgatccg ctccaccggg atgacggcct
     6781 tgcgttcgag gatcgcagcg tcctccatcg tgaacacgtc gttcgtcacg acgaccatct
     6841 cgagctcctc ggcgaatgcc cggcacagcg cgccgaccag ctcggtcttg ccgctgccga
     6901 ccggtccccc gatcccgatg cgcagcgcat caccggcatg ggtgtgccgg tgtgggtcgt
     6961 cgggtccgtg atgtgctccc atggcgatca gtgctcctgt caggtggcga agagacgcag
     7021 gtcccacgtc tcgtggtcga cggccgcgat ctcgcagcgc gggccggttc gggcgggaag
     7081 atcggcgagc ggcccgtgag cggctgcgac cgctgcggcg acgatcgcgt cggcctcggg
     7141 cgccaacgag accgcgatcg cgactgcggc gaacgggtcg aggcccagca gacgaacggc
     7201 agcctgggcc ggcgtattca acgcgtgatg caccgacacg gtcgcagccc cgacgggtga
     7261 ggcaccggac acgacggccg cagctccgag ggcgaccggc tggtgaacac cctcgaggtg
     7321 ggccgctgac aacgaggcga acagcggatc gggccagcac cgggcagcga cccgcgtgag
     7381 ctgccgaccg agcttgcgcg acgcggagcg caacggtggc ggcgcaatgc gagcttcgac
     7441 ctccgcatcg agctcggcga tcacggagcc caacaggtcg ctgccgtcgc tctgctcggc
     7501 atcgagccgg atcacactcg cggcgacgag cgccgcgtcg gtgagaccga cggtcgtcag
     7561 ccgaccccgc aggtagtcct cgagagaacg ctggcccgtg acccgaccgt cggcaacggc
     7621 cgactcgatg cccgccgagt gcacgtggcc cccggcgggg aaccg
//
LOCUS       DAOSOA010000135         7613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9637_length_7613_cov_5.348373, whole genome shotgun sequence.
ACCESSION   DAOSOA010000135 DAOSOA010000000
VERSION     DAOSOA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9637_length_7613_cov_5.348373"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1644
                     /locus_tag="PKY13_09615"
     CDS             148..1644
                     /locus_tag="PKY13_09615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT80233.1"
                     /translation="MTQTAIVVATALIVAYTLAMYVKGRGKSDADSGADDGSASPDLS
                     FVFMVPCLNEELVLGRTIDRLLAIESDHRFAVMVIDDGSTDGTAAVTESYDPDRVWLF
                     QRVAPNARQGKGEALNAAYRHLRQHVLTAGQSPDDVVVAVMDADGRLDPRALDLVAPH
                     FADPRRASVQIGVRIHNASDHVVARLQDMEFASYTELFQRGRSALGSAGLGGNGQFAR
                     LSALMSLGDAPWSDCLTEDLELGIEFLLGGWTNRFCHETHVSQQGIVEMRPLIKQRTR
                     WFQGHLQCTRFIGRILRSDLRPWPKFDLAFHLVNPLLMLLLQTASLVWLARLGWLVAT
                     QPAADTVMMFSGVRPVLLYLLAFGLAPVVTFVYLRAEPKLGIGAGIAFAHLYIFYGYM
                     WYFAGVRATVRHISRRRGWAKTARTTGTGDEARPTGFDVLPYLPASVRWFARIAEPPP
                     TPVARRTDVHIELVTLDGDPIGRLDELLAHRPAELPAGLPAERVGALR"
     gene            1695..3308
                     /locus_tag="PKY13_09620"
     CDS             1695..3308
                     /locus_tag="PKY13_09620"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOT80234.1"
                     /translation="MTNQPRTVRPRKMVRRSILLALAVITATFGIGLAEPEAERVADA
                     APQAAAPAPTTLVLYDSTGPYAWLGDIYAVQTANLAGHFGTVTRKKVTAYTKGDIAKY
                     TATIYLGSTYDEPLPAAFLSDVTSATKPVIWVADNIWQLTAATPTFAADYGWMWSGYD
                     TRPVSTVAYKGTTLTRSSLNNAGIMNSVVYDTAKANVVATAVADDGTQFPWAIRSKRL
                     TYVGENPYSYINETDRYLAWSDLLFDALAPQTAERHRAMIRIEDVGPTTDPTQLRQIV
                     DYLYSKKIKFTLATYSWYRDPRGVNNGGKAVSTYLYQSPQLTAALRYAQARGGTVIQH
                     GRTHQLDSLINPYDGVSANDFEFYRAHVDAATDAVVLDGPVANDSTANAKARIQSGRT
                     DFALALVSVSNIFEFPHYAASEADYQAVKALNLTAYDRRLYFPRNAAGKADTTRPTGQ
                     FFPYVVTDVYGTKVIPENMGNYEPEAYNQHPAVQIPELVQRARTSLVVRDGFASYFYH
                     PFLGVEPLKQLVEGVNAIGRYTWVGPNDV"
     assembly_gap    3333..3432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3433..4287
                     /locus_tag="PKY13_09625"
     CDS             <3433..4287
                     /locus_tag="PKY13_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80235.1"
                     /translation="IAATGASAVTIIPTWYVDDTSTSLPHRDPEQTASDASLRAAIVE
                     AHRVGLRVILKPHVDVEDDTDRAEIVPVDPAAWFDRYRDLMVGYANLATETGVEMLSV
                     GTELAGTMDRPVEWADVIAAIRSVYSGPLTYASNFDGYTDVPFWDRLDDIGVDAYFEL
                     STEPTTDVDRLVTAWGPVLDELDTFAAGLGKPVIFTEAGYASQVGSTTQPWNWEISEE
                     RSDAEQAAGYEALFTAVADRSWIDGVQWWMWDDLADTGEDQALDYTPHGKAAERVLRS
                     QWAVGDER"
     gene            4287..6017
                     /locus_tag="PKY13_09630"
     CDS             4287..6017
                     /locus_tag="PKY13_09630"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOT80236.1"
                     /translation="MRRVTTMAAVGTVAVVAAVVLGVGGGADPLAPSDGSSAVVPESE
                     PGSASGSGLASVSPTTTRPERAALPMLVLYDTSGEWSAVGQTDAILSANLLGHFGPVT
                     RLPIGDYTAGMAASFGGVLYVGTTFDEPIPTAFLDDVVGGPTPIVWAGLNIWQLGDRL
                     TAGGYGFTPGLLDARPMAKVRYEGAEFDRAASGDSNEVVTLTVDNPAVATTLGTIVAE
                     DGTEFPWGVRARNLTYLAESPYTFLSERDRYVAWADVLLDAFTPGTPERHRALVRLED
                     IGPASDPVLLRASIDVLVKRRIPFTMAVYPLYADPTGVHGTKYGSKGIPALRLDQRPK
                     VVAAITYGLEHGGTIVSHGLTHQYGKVDNPYDGVSANDFEFFRAHVDPATDDVVLDGP
                     VEEDSVEWAADRLRTARTMLAKAGIPLDEVRMFEPAHYTASDATYRAIGEVYAGLNSG
                     GPATRFDRGMYFAPGESGDLDADHPIGQFFPFAVRDVYGHLVVPENLGNVIEVGYNNN
                     DPRTPEEIVENARRSRVVRDNVVGFFFHPYLDADLLAETVDGIVAAGHEFVPSTSVTT
                     GWDMPSSSPP"
     gene            6044..7576
                     /locus_tag="PKY13_09635"
     CDS             6044..7576
                     /locus_tag="PKY13_09635"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT80237.1"
                     /translation="MTDAPDAPLAAALARAPQGNLHKEAEKLARQNKLFVRERIDLLC
                     DPGTFVEDGLLANASATDLPADGVVTGVGEVDGRAAVIVANDPTVKAGSWGARTVEKI
                     VRMTDIALRDNLPVFWLIDSAGARITDQVELFPGRRGAGRIFYNQVRLSGKVPQICCL
                     FGPSAAGGAYIPSFCDIVIMVEGNASMYLGSPRMAEMVIGEIATLEETGGARMHCSVS
                     GCGDNLAHDDADAIEQAKAYFTYMPTYWRHDPPTYEAVAPAHDLDDSIVPAEDTMGYD
                     IHRVIDCLVDAESFFEVKPLFAPEMVVGFGLMDGSPVGFVANNPMIKGGVLFTDTADK
                     AARFIWCCDAFNIPLIFLADVPGFMIGTEVERQGIIRHGAKMITAVSEATVPKISVVL
                     RKAYGAGLYAMSGPAFEPEATIALPTAKIAVMGPEAAVNAVFANKIAAIEDLDEREAF
                     ILEQRRIYEEDVDLLRLGSELVIDAVVQWGDLRTEIIRRLTRAAGKTRVFTDRRHGVP
                     PV"
BASE COUNT         1189 a         2719 c         2439 g         1166 t
ORIGIN      
        1 cgaactgatc agctcctcga cggagcgcac cccgggcgtc gctgcgacgt tgaccggggc
       61 caacgcaccg ctgctcaccg tggcggggtt cctgctcatc gcgctcgccc tcgtggctgt
      121 ggcgtggacg gcgaagcacc cacgtcgatg acccagaccg ccatcgtcgt ggcgaccgcc
      181 ctgatcgtcg cgtacacgtt ggcgatgtac gtgaagggcc ggggcaagtc cgacgcggac
      241 agcggcgccg acgacggttc ggcgtccccg gacctgtcgt tcgtcttcat ggtgccgtgc
      301 ctcaacgaag agctggtgct cggccggacc atcgaccgtc tcctggcgat cgagagcgac
      361 caccgttttg ccgtgatggt gatcgacgac gggtcgaccg acggaaccgc ggcggtcacc
      421 gagtcgtacg accccgaccg tgtgtggctc ttccagcggg ttgcgcccaa cgcccggcag
      481 ggcaagggcg aggcgctcaa cgccgcgtat cgccacctcc gccagcacgt gctgaccgcc
      541 gggcaatcgc ccgacgacgt cgtggtcgcg gtcatggacg ccgacggccg gctcgacccg
      601 cgggcgctcg acctcgtcgc gccgcacttc gcggatcctc ggcgggcgtc ggtgcagatc
      661 ggcgtccgga tccacaacgc gtccgaccac gtcgtcgcac gtctccagga catggagttc
      721 gcgagctaca cggagctctt ccaacgtggc cgctccgcgc tggggtcggc ggggctcggc
      781 ggcaacggcc agttcgcccg gctcagcgcc ctcatgtccc tcggtgacgc gccgtggtcc
      841 gactgcctca ccgaggacct cgagctcggc atcgagttcc tcctgggcgg ttggacgaac
      901 cggttctgcc acgagaccca cgtcagccag cagggcatcg tcgagatgcg accgctgatc
      961 aagcagcgga cgcgctggtt ccagggccac ctgcagtgca cccggttcat cggccggatc
     1021 ctgcgctcgg acctgcggcc gtggccgaag ttcgacctgg cgttccacct ggtgaacccg
     1081 ctcctgatgc tgctcttgca gaccgcgtcg ttggtctggc tggcgcgact cggctggttg
     1141 gtggccaccc agcccgccgc cgacacggtg atgatgttct ccggcgtccg tccggtgctg
     1201 ttgtacctgc tggcgttcgg cctcgcgccc gtcgtcacct tcgtgtacct gcgcgccgag
     1261 cccaagctcg gcatcggggc cggcatcgcg ttcgcccacc tctacatctt ctacgggtac
     1321 atgtggtact tcgccggcgt gcgggcgacg gttcgtcaca tcagccgccg ccgcgggtgg
     1381 gccaagacgg cacgcaccac cgggaccggc gacgaggctc gcccgaccgg gttcgacgtg
     1441 ttgccctacc tcccggcatc ggtccgctgg ttcgcccgga tcgccgagcc gccgccgaca
     1501 cctgtcgccc gtcggaccga cgtccacatc gagctggtga cgctcgacgg cgacccgatc
     1561 ggtcgactcg acgagctgct cgcccaccgc ccggcggaac tccccgcggg gcttcccgcc
     1621 gaacgagtcg gagcgctccg atgaccacct accccgaaac ccgatccacg accaccgaga
     1681 ccaggagtcc cgcaatgacg aatcaacctc ggaccgttcg gcctcgaaag atggtgcgcc
     1741 gctcgattct gctcgcactg gccgtcatca ccgcgacgtt cgggatcgga ctcgccgagc
     1801 ccgaggccga gcgtgtcgcc gatgcggcac cccaggctgc cgcgccggct ccgacgacgc
     1861 tcgtgttgta cgactcgacg gggccgtacg cctggctggg cgacatctac gccgtgcaga
     1921 ccgccaacct cgccggccac ttcgggaccg tgacccgcaa gaaggtcacc gcgtacacga
     1981 agggcgacat cgccaagtac accgccacga tctacctcgg ctcgacctac gacgagccgc
     2041 tgcccgctgc attcctgtcc gacgtgacgt cggccacgaa gccggtcatc tgggtcgctg
     2101 acaacatctg gcaactgacc gcagcgaccc cgaccttcgc cgccgactac ggctggatgt
     2161 ggtccggata cgacacccgc ccggtgtcga cggtcgccta caagggcacg accctgaccc
     2221 gctcgtcgct caacaacgcc ggaatcatga actcggtcgt ctacgacacc gccaaggcca
     2281 acgtggtggc gacggcggtc gccgacgacg gcacccagtt cccgtgggcg atccgctcca
     2341 agcgcctcac ctacgtcggc gagaatccgt acagctacat caacgagacc gaccgctacc
     2401 tggcctggtc ggacctgctg ttcgacgccc ttgccccgca gactgccgag cggcatcggg
     2461 cgatgatccg catcgaggac gtcggcccca cgaccgaccc cacccagctc cgtcagatcg
     2521 tcgactacct gtacagcaag aagatcaagt tcaccctcgc cacctacagc tggtaccgcg
     2581 accctcgggg cgtcaacaac ggcggcaagg ccgtcagcac gtacctgtac cagtcgccgc
     2641 agctgacggc ggccctccgg tacgcccagg cccgcggtgg caccgtcatc caacacggcc
     2701 ggacccacca gctcgactcg ctgatcaacc cctacgacgg cgtgtcggcc aacgacttcg
     2761 agttctaccg cgcccacgtc gacgcagcga ccgacgccgt ggtcctcgac gggccggtcg
     2821 ccaacgactc gaccgccaac gcgaaggcgc ggatccagtc gggccgcacc gacttcgcgc
     2881 tcgccctcgt ctcggtgtcg aacatcttcg agttcccgca ctacgcggcg tcggaggccg
     2941 actaccaggc ggtgaaagcg ctgaacctga cggcgtacga tcggcgtctg tacttccccc
     3001 gcaacgccgc cgggaaggcc gacacgaccc gtccgaccgg ccagttcttc ccgtacgtcg
     3061 tcaccgacgt gtacggcacc aaggtcatcc cggagaacat gggcaactac gagccggagg
     3121 cgtacaacca gcatccggcg gtgcagatcc ccgagctggt gcaacgggcc cgcacgtcgt
     3181 tggtggtccg tgacggtttc gcgagctact tctaccaccc gttcctgggc gtcgaaccgc
     3241 tcaagcagct cgtcgagggc gtgaacgcga tcgggcggta cacctgggtc gggccgaacg
     3301 acgtctgatg ccgtcgacgg ctccccgcct cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnatcgcggc caccggcgcg agcgcggtca cgatcatccc gacctggtac
     3481 gtcgacgaca cgtcgacgtc gttgccccat cgtgaccccg agcagacggc gagcgacgcc
     3541 agcctgcgcg ccgcgatcgt cgaggcgcac cgtgtgggtt tgcgggtcat cctcaagccg
     3601 catgtcgacg tcgaggacga caccgatcgt gccgagatcg tgcccgtcga ccccgcggcc
     3661 tggttcgatc gctaccgcga cctcatggtc ggctacgcga acctcgccac cgagaccggg
     3721 gtggagatgc tcagcgtcgg caccgaactg gccggcacca tggatcggcc cgtcgagtgg
     3781 gccgacgtga tcgcagcgat ccgctcggtc tacagcgggc cactcacgta cgcctcgaac
     3841 ttcgacggct acaccgacgt cccgttctgg gatcgtctcg acgacatcgg cgtcgacgcg
     3901 tacttcgagc tgtcgaccga acccacgacc gacgtggacc gactggtgac cgcctggggc
     3961 cccgtgctcg acgaactcga tacgttcgcg gccggtctcg gcaagccggt gatcttcacg
     4021 gaagccggat acgccagcca ggtggggtcg accacccagc cgtggaactg ggagatcagc
     4081 gaggaacggt ccgacgccga acaggccgcc ggatacgagg ccttgttcac ggccgtcgcc
     4141 gaccgttcgt ggatcgacgg tgtgcagtgg tggatgtggg acgacctggc cgacaccggg
     4201 gaggaccagg ccctcgacta caccccacac ggcaaggcgg ccgagcgggt gctgcgctcg
     4261 cagtgggcgg tcggtgacga acgctgatgc ggcgcgtcac cacgatggcg gcggtcggta
     4321 cagtggcagt cgtcgctgcc gtggtgctgg gcgtcggtgg gggagccgat cccctcgctc
     4381 cgtcggacgg ctcgtcggca gtcgtgccgg agtcggagcc cgggtcggcg tcggggtcgg
     4441 ggttggcttc ggtgtcgccg acaaccaccc gcccggaacg ggcggcgctg ccgatgttgg
     4501 tgctgtacga cacgtcgggg gagtggtcgg cggtcggcca gaccgacgcc atcctgtcgg
     4561 cgaatctcct cggacacttc ggcccggtca cccgtctgcc gatcggtgac tacaccgccg
     4621 gtatggcggc gtccttcggc ggtgtgctct acgtggggac gacgttcgac gagccgatcc
     4681 ccacggcgtt cctcgacgac gtcgtcggcg ggcccacccc gatcgtgtgg gcgggtctca
     4741 acatctggca gctcggggac cggctgaccg ccggcgggta cggcttcact cccggactcc
     4801 tcgacgcgcg cccgatggcg aaggtgcgct acgagggtgc cgagttcgac cgcgctgcct
     4861 ccggcgactc caacgaggtc gtgacgctga ccgtcgacaa cccggcggtg gcgacgacgc
     4921 tcggcacgat cgtcgccgag gacggcacgg agttcccgtg gggcgtacgc gcccgcaacc
     4981 tgacctacct cgcggaatcg ccctacacgt ttctctccga acgcgaccgc tacgtcgcct
     5041 gggccgacgt cctcctcgac gccttcaccc ccggcactcc cgaacgacac cgagccctgg
     5101 tccggctcga ggacatcgga ccggccagtg atccggtcct cctgcgggca tccatcgatg
     5161 tcctcgtgaa gcggcggatt cccttcacga tggcggtcta ccccctgtac gcggacccga
     5221 ccggcgtgca cggcaccaag tacggctcga agggcatccc ggcgcttcgg ctcgaccagc
     5281 gcccgaaggt ggtcgccgcc atcacctacg gcctcgagca cggcggcacg atcgtgagcc
     5341 acgggctgac ccaccagtac ggcaaggtcg acaaccccta cgacggggtg agcgccaacg
     5401 acttcgagtt cttccgtgcc cacgtggacc cggcgaccga tgacgtcgtc ctcgacggcc
     5461 ccgtcgagga ggattccgtc gagtgggccg ccgaccggct gcgcacggcg cgcacgatgc
     5521 tcgcgaaggc agggattccg ctcgacgagg tccggatgtt cgaaccggcg cactacaccg
     5581 cctccgacgc cacctatcgg gccatcggtg aggtctacgc gggcctcaac agcggcggac
     5641 cggcaacgag gttcgaccgc gggatgtact tcgccccggg cgagtcgggc gatctcgacg
     5701 ccgaccaccc gatcgggcag ttcttcccct tcgcggtgcg tgacgtctac gggcacctcg
     5761 tcgtccccga gaacctcggc aacgtcatcg aggtcgggta caacaacaac gaccctcgga
     5821 cgcccgagga gatcgtcgag aacgcacggc gcagccgcgt ggtccgcgac aacgtcgtcg
     5881 gcttcttctt ccacccgtac ctcgacgcag atctcttggc cgagaccgtc gacggcatcg
     5941 tcgcagcggg gcacgagttc gtgccgtcca cctcggtgac gacggggtgg gacatgccga
     6001 gcagttcgcc gccgtgaccc ccgagccgcc aaactcgtac cccatgaccg acgcacccga
     6061 cgccccgctc gccgccgcac tggcccgtgc tccccagggc aacctccaca aggaggccga
     6121 gaagctggcg cgccagaaca agctgttcgt gcgggaacgc atcgacctcc tgtgcgaccc
     6181 cgggaccttc gtcgaggacg gcctgctcgc caacgcgagc gccaccgacc tgccggccga
     6241 cggcgtcgtc accggcgtcg gcgaggtcga cggccgcgcc gccgtcatcg tcgccaacga
     6301 ccccaccgtg aaggcgggct cgtggggcgc ccgcaccgtc gagaagatcg tgcgcatgac
     6361 cgacatcgcc ctccgcgaca acctgccggt cttctggctg atcgactcgg ccggcgcccg
     6421 catcaccgac caggtcgagt tgttccccgg tcgtcggggc gccgggcgga tcttctacaa
     6481 ccaggtgcgg ctgtcgggga aggtgcccca gatctgctgc ctgttcggcc cgtccgctgc
     6541 cggcggggcg tacatcccgt cgttctgcga catcgtgatc atggtcgagg gcaacgcctc
     6601 gatgtacctc ggctcacccc gcatggccga gatggtcatc ggtgagatcg ccaccctcga
     6661 ggagaccggc ggcgcacgga tgcactgctc ggtgtcgggg tgcggcgaca acctggccca
     6721 cgacgacgcc gacgccatcg agcaggccaa ggcgtacttc acgtacatgc cgacctactg
     6781 gcgccacgac ccgcccacgt acgaggccgt cgctcccgcc cacgacctcg acgattcgat
     6841 cgtgcccgcc gaggacacca tgggctacga catccaccgg gtcatcgact gtctcgtgga
     6901 cgccgagagc ttcttcgagg tcaagccgtt gttcgcgccg gagatggtcg tcgggttcgg
     6961 tctgatggac ggctcgccgg tcgggttcgt ggcgaacaac cccatgatca agggtggtgt
     7021 gctcttcacc gataccgccg acaaagcggc ccggttcatc tggtgctgcg acgcgttcaa
     7081 catcccgctg atcttcctgg cggatgtgcc cggcttcatg atcggcaccg aggtggagcg
     7141 ccaggggatc atccgccacg gggcgaagat gatcaccgcc gtgtcggagg cgacggtgcc
     7201 gaagatctcg gtggtgctcc gcaaggcgta cggcgccggt ctctacgcca tgagcggacc
     7261 ggcgttcgag ccggaggcga cgatcgccct gccgaccgcc aagatcgccg tgatgggccc
     7321 cgaggcggcc gtcaacgccg tgttcgccaa caagatcgcg gcgatcgagg acctcgacga
     7381 acgcgaggcg ttcatcctcg agcagcgacg gatctacgag gaggacgtcg acctgctgcg
     7441 gctgggttcg gagctcgtca tcgacgccgt cgtgcagtgg ggtgacctgc gcaccgagat
     7501 catccggcgc ctgacccgcg ccgccggcaa gacccgcgtc ttcaccgacc gtcgccacgg
     7561 cgtgccgccg gtctgacctg cgttctgcgg gtttgatttg gcgtggttgc tat
//
LOCUS       DAOSOA010000136         7585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9681_length_7585_cov_6.834529, whole genome shotgun sequence.
ACCESSION   DAOSOA010000136 DAOSOA010000000
VERSION     DAOSOA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9681_length_7585_cov_6.834529"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..549)
                     /locus_tag="PKY13_09640"
     CDS             complement(43..549)
                     /locus_tag="PKY13_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80238.1"
                     /translation="MATRTRESEAGFEAAFRELYPRAFGLALRMLGNRAVAEDLAAET
                     MARAFAQWGRLDPDRRVGWVLRVTSNQAIDLLRKKGHTMVPEVIDLEDGTALRIALAA
                     ALAQLPRRQREAIALRYLSDLTEAETAQALGIGVGSVKTHTHRGLAALRSKFGAEFED
                     RQMEQLGV"
     gene            complement(706..1557)
                     /locus_tag="PKY13_09645"
     CDS             complement(706..1557)
                     /locus_tag="PKY13_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80239.1"
                     /translation="MSRSVLRRATWLGVLRSAAVAIVIVVVFDAVWTRVGAPAIATRH
                     GRDRVIEEQIRGSLQSLSPSSLVAPPTRTVGRDGSLRYQFFLLDPVPGDNPRQRMFEF
                     EVSSLGVVDVDDGAFSMAISSSETLRFRDASRRSAVSFVAAVSQLPPGAVVSFALYLP
                     SPLDDGDAQALWRALEVDGVMPSGYLMAPDQQGNPRGWSGPELDQYRRWDAAMSRDDG
                     VETGSSNVGITGANAGRAPDAAVIGLVYTNLPADRVAVAIDRTGATETASGPVGLSLE
                     EFTSPSR"
     gene            complement(1692..2195)
                     /locus_tag="PKY13_09650"
     CDS             complement(1692..2195)
                     /locus_tag="PKY13_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80240.1"
                     /translation="MRSPDPVERLFVAHRSTVFGYLARRSGDRELASDLTQETFVRVA
                     RNLNGFSGGSETAWLLTIARNLLIDHWKRRRLPINDRALIDALAGDEQLEDAATTRLF
                     VHHTLTQLEPRDRRLLTLLYLEGFTTREVAAMAGTTDGSVRMAALRARDAFRVHAANQ
                     HQGSETT"
     gene            complement(2717..3664)
                     /locus_tag="PKY13_09655"
     CDS             complement(2717..3664)
                     /locus_tag="PKY13_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT80241.1"
                     /translation="MALSDEVRDLGKKLSNWGRWGDDDQLGCGNFLTPEAARRGAACV
                     RTGKRFSLALDLKHDGVQTGQPKGRFNPILTFTSLNERDKMAPGIWEGTDDLVTMSTC
                     AATHIDALSHISYDGLLYGGRSQKTIGANNGATWCGAEQLPDIVTRGILLDVARALGV
                     DRVDPGHAVTAADLDAALAATGLTVESGDVICIRTGDIGWYHDGDRRRYAVGVDWKTV
                     GLGFSCVEWFAEREVAGAFVDTYTYEVMPPESGNWDDLLAVHMVQLRDMGLLQGQNWD
                     FEALAVDCAEDGVSEFLLVAAPEPIVGATSAPVVPIATK"
     gene            complement(3683..4768)
                     /locus_tag="PKY13_09660"
     CDS             complement(3683..4768)
                     /locus_tag="PKY13_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT80242.1"
                     /translation="MRAIVWDGKQASVESGIEARDLRAGEVRVRIEAAGLCHSDVSVL
                     NGTIMFPPPVVLGHEGAGEVIQIAPDVTNVAVGDHVVLSTLGNCGQCGQCDRGKPTFC
                     RQSLGKLSRPFTSGEGEEQKKLFQFANLGVFAEETVVKATQAVRIPKEVPLTSASLIG
                     CGVLTGVGAVFNRAKVQQGESVVVIGVGGIGLNAIQGAALSGGLPVIAVDTNPGKEAF
                     ARQFGATHFICPDGPDFDLVAAVKEICPNGVDYVFECVGSTALIKTSTDLLDWGGTLV
                     MLGVPKMGSEASFVVNTMYNDKTIMGCRYGSARPQYDIPLMVRLYQAGRLKLDELVSQ
                     TYPLEDFNLALDELHEGKLARGVLTFA"
     gene            complement(4827..6104)
                     /locus_tag="PKY13_09665"
     CDS             complement(4827..6104)
                     /locus_tag="PKY13_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80243.1"
                     /translation="MVTIASSPLSDEHHETSSAEPTPSLDQFLVEARVFLDANATPKP
                     AVAAEFVWGEGDDFVGIVEEGDPETEVEQLAAARAWAATRFDAGFGWIDGPIELGGRG
                     LGADHVRAYRSLESAYELPDQSIFTIGLGMVAPTISAHATPEVAAQYLQPLQRGDLIA
                     CQLFSEPGAGSDLAAVATRAERDGDDWIVNGQKVWTSNAHHAGLGEIICRTDPDAPKH
                     AGMTAFLVDMAAPGVEVRPLRQMTGGAGFNEVFFSDVRIPDTHRLGEVGQGWGVAMTT
                     LMNERASIGSGMGLGRGPGPFERIIALTREMGRQDDPVIRDRLAQLYIQHKVIGYTGK
                     RALAAVKAGKLPGPEMSILKLAGTLQLLDTAEFAAQVLGPRLCADTGEWGTFAWTKLV
                     CGAPGGRLGGGTDEILRNIIGERVLGLPREPKG"
     gene            complement(6147..6758)
                     /locus_tag="PKY13_09670"
     CDS             complement(6147..6758)
                     /locus_tag="PKY13_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT80244.1"
                     /translation="MPPRPRTRLDPDLRRGQILGAAADIFREQEFSAVSLDAVAAAAG
                     VTRGLLHHYFGSKRGLYLAVVEQAVRVPESARIVPDDTSGDLDEVLGACVESWMRMIE
                     AAGGLWSGSAPAGGFAVSDVDEVITRARDDLVERMVDELPFPASLDPDLLRGALRSYA
                     ALARVASQEWLVAGTLTRAQTAAMLHASLLAIVETVVPRMMEA"
     gene            complement(6783..>7585)
                     /locus_tag="PKY13_09675"
     CDS             complement(6783..>7585)
                     /locus_tag="PKY13_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80245.1"
                     /translation="VTPTSVRDRVLATAVVGGGPGGLGPRRWRGDGFPPHPDRARRPG
                     GFAVAAVVVLVLVIAGLWALPADDATTVATSDRRGGSTTSVTSTTATPRVTTSAPLPS
                     STVPLVVDTAAPTTVLRPTSTRVVAPPTTVPLASTSTTSVPTTTTSAPASTTTTTAAP
                     PTLRLSGPSALSARAAGGGACANTAFAATITSGGIPTSVTLRWIAGTTSGSVAMVRNR
                     TRNGWNGTIDLPYGSTGAVTIVAEATSGGATGRSNTLTATGNPCPVIE"
BASE COUNT         1225 a         2577 c         2591 g         1192 t
ORIGIN      
        1 tcgaggtcga ggtcgtcgac cgatctggtg atgtgctcgt gctcagacac ccagctgctc
       61 catctgtcga tcttcgaact ccgcgccgaa cttcgagcgg agtgcggcaa ggccgcgatg
      121 ggtgtgcgtc ttgaccgagc cgacaccgat tccgagcgcc tgcgcggtct cggcttcggt
      181 caggtcgctc aagtagcgga gcgcgatcgc ctcccgctga cgccgcggca gctgcgccag
      241 cgcagcggcc agcgcgatcc ggagggcggt gccgtcctcg aggtcgatga cctcgggaac
      301 catcgtgtga cccttcttgc gcagcaggtc gatcgcctgg ttcgacgtca cccggagcac
      361 ccagccgacc cggcggtcgg ggtcgaggcg accccactgg gcgaacgccc gcgccatcgt
      421 ctcggcggcc aggtcctccg ccaccgcacg gtttcccaac atgcgcaacg ccaggccgaa
      481 ggcccgggga tacaactccc ggaaggcggc ctcgaagccc gcctcgctct ccctcgttcg
      541 tgtcgccatc gcacccatca tgaaggagta cgtccgaccc caccagaagg ttgacgcgat
      601 cccgaaagaa tccgtcgggc cgggttgagc gcgacgaggg ggctctggcc ctacgacggg
      661 tcccgtgctt ctgtacgacg ttgtgagttc ggggtggaac gacgactatc gcgacggtga
      721 ggtgaattcc tcgaggctca gcccgaccgg acctgacgca gtctcggttg cgccggtccg
      781 gtcaatcgcg accgcgacac ggtcggccgg aagattggtg tacacgagtc cgatgaccgc
      841 agcatcgggt gcccgaccgg cgttggcgcc ggtgatcccg acattgctcg acccggtctc
      901 aaccccatcg tcgcggctca tcgcggcgtc ccagcggcgg tactggtcga gttccgggcc
      961 cgaccacccg cgagggttcc cctgctggtc gggggccatg aggtagccgc tcggcatcac
     1021 tccgtcgacc tccagtgccc gccagagggc ttgagcatcg ccgtcgtcga ggggcgacgg
     1081 caggtacaga gcaaacgaaa cgactgctcc gggcggaagc tgcgagacgg cagcgacgaa
     1141 ggagacagcc gagcgtcggg acgcgtcgcg gaatcgaagc gtttcagacg acgagatcgc
     1201 catcgagaac gctccgtcgt cgacatcgac gactcccaat gaggacacct cgaactcgaa
     1261 catccgttgc cgagggttgt caccggggac ggggtcgagg aggaagaact ggtagcggag
     1321 cgacccatcg cgcccgaccg tgcgcgtcgg aggcgccacg agactcgaag ggctcagcga
     1381 ttgaagggat ccacggatct gttcctcgat cactcggtcg cgaccgtgac gggtcgcaat
     1441 cgccggcgct ccgacccgcg tccacactgc gtcgaacaca acgacgatca cgatcgcgac
     1501 cgccgccgac cgcagaacgc ccagccatgt ggcgcgccgc aggactgatc ggctcatcac
     1561 aggaaggtcg ccgaggtcgg ggtgaaggtc gacgtccaga tcgtctcggt ccagagggtc
     1621 ccggtccgga tcctcgatgt tcacaggctc cggaatcccg tcgatcggcg ctcgtctgac
     1681 tgggtcgtcg ttcatgtcgt ctcgcttcct tggtgctggt tcgccgcatg gacgcgaaac
     1741 gcgtcccggg cacgaagtgc tgccattcgc acgcttccgt cggtggttcc agccattgcc
     1801 gcgacttctc tggtcgtgaa gccctcgagg tagaggagtg tcaggagtcg acggtcacgg
     1861 ggctcgagtt gggtgagcgt gtggtgaaca aacagccgcg tggtcgcggc atcttcgagt
     1921 tgttcgtcgc cggcaagagc gtcgatgagt gcacggtcgt tgatcgggag tcgtcgccgc
     1981 ttccagtggt cgatcaacag attgcgggcg atcgtcagga gccacgccgt ctcggaaccg
     2041 ccgctgaagc cgttcaggtt tcgtgccacc cggacgaacg tctcctgggt gaggtcggag
     2101 gcgagttctc gatcgccgct gcgccgtgcc agatatccga acacggtcga gcggtgagcg
     2161 acgaagagcc gctctaccgg atcaggagat ctcaccggag gatcgtaggt tcagtacccg
     2221 acccacgact gcgacgtcgc gccgtcctcg aacgtcgtgc ggcacaccca cctcggccgc
     2281 ccttccacga ctgcggggac gacagattgg cgtgaaaccg ctgcaggtgc gtacgagatt
     2341 cccaggtcga tcccacagat ctcggccagg gacggccgag taacccacag tcctgccagg
     2401 gtcatgctga ccagaccaac gaaagccacg actttcgtcc gccgccggac catcggtcgg
     2461 cgagtggacg gcatgtccgc tggcccggca gttgtcgtgg atgatgccgc ttcgagtgtc
     2521 tcggtcacgt gggttctccc tggggtcggt cggcgttgat cgccgactct gccaagtgga
     2581 aacgactcag ccgagctcgc gtcacaagaa tccgaaggtt ttctcgacga ggttgtgatc
     2641 atcagtgtcg acatcacctc atgggctcac cggctcatgg cctcgactcc gaccgttgtc
     2701 gctgttgtcg agtgggctac ttggtggcga tgggtacgac gggggccgac gtggcgccga
     2761 cgatcggctc cggtgcggcg accaggagga actcggacac gccgtcctcg gcgcagtcga
     2821 cggccagcgc ctcgaagtcc cagttctgac cctgcagcag acccatgtcg cggagctgca
     2881 ccatgtgtac ggccaacagg tcgtcccagt tgcccgactc ggggggcatg acctcgtacg
     2941 tgtacgtgtc gacgaaggcg ccggcgacct cacgctcggc aaaccactcg acgcagctga
     3001 atccgagacc gaccgtcttc cagtcgaccc ccaccgcgta gcgacgacga tcaccgtcgt
     3061 ggtaccagcc gatgtcgccg gtgcggatgc agatgacgtc gcccgactcg acggtgaggc
     3121 cggtggcggc gagggccgcg tcgaggtcgg cggcggtcac ggcgtgaccc gggtcgaccc
     3181 gatcgacccc gagcgcccgg gcgacgtcga gcaggatgcc gcgggtcacg atgtcgggga
     3241 gttgttcggc cccacaccac gtcgcgccgt tgttggcgcc gatcgtcttc tgcgaacgtc
     3301 cgccgtagag gaggccgtcg tagctgatgt ggctgagggc gtcgatgtgg gtcgccgcgc
     3361 acgtcgacat cgtgacgagg tcgtcggtgc cctcccagat accgggcgcc atcttgtcgc
     3421 gctcgttcag cgaggtgaac gtgaggatcg ggttgaaccg gcccttcggt tgcccggtct
     3481 gcaccccgtc gtgcttgaga tcgagggcga gcgagaagcg cttgcccgtc cgcacgcacg
     3541 cggcgccgcg ccgcgccgcc tcgggggtga ggaagttgcc gcagccgagc tggtcgtcat
     3601 cgccccatcg gccccagttc gaaagcttct tgcccaggtc acggacctcg tcggaaagtg
     3661 ccatgcgtcg atgtccgccg gctcaggcga acgtgagcac gccgcgggcc agcttgccct
     3721 cgtgcagctc gtcgagcgcg aggttgaagt cctcgagcgg gtacgtctgc gacaccaact
     3781 cgtcgagctt gagacgaccg gcctggtaga ggcggaccat cagcgggatg tcgtactgcg
     3841 ggcgcgccga gccatagcgg caccccatga tcgtcttgtc gttgtacatc gtgttgacca
     3901 cgaagctggc ctcgctgccc atcttgggca caccgagcat gacgagggtg ccgccccagt
     3961 ccagcaggtc ggtcgacgtc ttgatgaggg ccgtcgagcc gacgcactcg aagacgtagt
     4021 cgacaccgtt ggggcagatc tccttgacgg cggcgaccag atcgaagtcg ggaccatcgg
     4081 ggcagatgaa atgcgtggcg ccgaactgac gggcgaacgc ctccttgccg gggttggtgt
     4141 cgacggcgat gaccggcagg ccgccgctga gcgctgcgcc ctggatggcg ttgaggccga
     4201 tgccaccgac cccgatgacc acgaccgatt cgccctgctg caccttggcc cggttgaaca
     4261 ccgcgccgac gccagtgagc acaccgcacc cgatgagcga cgccgacgtg agtggcacct
     4321 ccttggggat gcgcaccgcc tgggtcgcct tcacgacggt ctcctcggcg aagaccccga
     4381 ggttggcgaa ctggaacagc ttcttctgct cctcaccctc gccgctggtg aagggccgag
     4441 agagcttgcc gagcgactgc cgacagaacg tgggcttgcc gcggtcgcac tgcccgcact
     4501 gcccgcagtt gccgagcgtc gacaacacca cgtggtcacc gacggcgacg ttggtgacgt
     4561 caggagcgat ctggatgacc tcacccgcgc cctcgtggcc gagcacaacg gggggcggga
     4621 acatgatcgt gccgttcagc accgacacgt cgctgtggca gaggccggcg gcctcgatgc
     4681 gcacccggac ctcaccggca cgcaggtcgc gagcctcgat tcccgactcg accgatgcct
     4741 gcttgccgtc ccacacgatt gctctcatgg ggagcgatgg tagccgggaa ctagaacgcg
     4801 tttcatttgg gccgacgtcc ccgggatcag cccttcggct ctcggggcag gcccagcacc
     4861 cgctcgccga tgatgtttcg caggatctcg tcggtccccc cgccgaggcg cccgccgggt
     4921 gcaccgcaca ccagcttcgt ccacgcgaac gtgccccact ccccggtgtc ggcgcacagc
     4981 ctcgggccga gcacctgcgc cgcgaactcg gcggtgtcga gcagttggag cgtgcccgcc
     5041 agcttgagga tcgacatctc gggcccgggc agcttgccgg ccttcaccgc tgcgagcgcc
     5101 cgtttgccgg tgtacccgat caccttgtgc tggatgtaga gctgcgccaa ccggtcgcgg
     5161 atcacggggt cgtcctgcct gcccatctcc cgggtgaggg cgatgatccg ctcgaacggt
     5221 ccgggtccac ggccgagacc catgccgctc ccgatcgacg cccgttcgtt catcagcgtc
     5281 gtcatggcga cgccccagcc ctggccgacc tcgccgaggc gatgggtgtc cggaatgcgg
     5341 acgtcgctga agaacacctc gttgaacccg gcgccaccgg tcatctggcg aagcggtcgc
     5401 acctcgacgc cgggcgccgc catgtcgacg aggaacgcgg tcatgccggc gtgcttggga
     5461 gcgtcggggt cggtccggca gatgatctcg ccgagcccgg cgtgatgagc gttcgacgtc
     5521 cagaccttct gcccgttgac gatccagtcg tcgccgtcgc gttcggcgcg ggtggcgacc
     5581 gcggcgagat cggaacccgc gccgggttca ctgaacagct ggcaggcgat caggtcgcca
     5641 cgctgcagcg gctggaggta ctgcgccgcc acctcgggcg tggcgtgtgc ggagatcgtc
     5701 ggggccacca tgccgagacc gatcgtgaag atcgactggt cggggagctc gtaggcggac
     5761 tcgagcgacc ggtacgcccg gacgtggtcc gctcccagcc cgcgaccgcc gagctcgatg
     5821 ggcccgtcga tccaaccgaa cccggcatcg aagcgcgtgg ccgcccacgc gcgggccgct
     5881 gccagctgtt cgacctcggt ctcggggtcg ccctcctcga cgatgccgac gaagtcgtcg
     5941 ccttcgcccc acacgaactc ggcggcgacg gccggcttgg gcgtcgcgtt cgcgtcgagg
     6001 aacacccgcg cctccacgag gaactggtcg agcgacggtg tgggctcggc ggaggaggtt
     6061 tcgtggtgtt cgtcgctcaa cggagacgag gcgatggtga ccatcgtcac attccagcac
     6121 actattggcg aagtgccaat agtgtcctag gcctccatca tcctggggac gaccgtctcc
     6181 acgatggcca gcagggaggc gtgcagcatc gccgccgtct gcgcccgcgt cagcgtgccg
     6241 gccaccagcc actcctgcga tgcgacacgc gccagcgcgg cgtaggagcg aagcgccccg
     6301 cgcagcagat cgggatcgag ggaggcgggg aacggcaact cgtcgaccat ccgctcgacg
     6361 agatcgtccc gcgcccgggt gatcacctcg tcgacgtcgg acaccgcgaa gccgcccgcc
     6421 ggggccgaac ccgaccagag gccgccggcc gcctcgatca tccgcatcca gctctcgacg
     6481 cacgctccga gcacctcgtc gaggtcaccg ctcgtgtcgt ccggaacgat ccgagccgat
     6541 tcgggaacgc ggaccgcctg ctcgaccacg gcgagataca gcccgcgctt ggacccgaag
     6601 tagtggtgga gcagacccct cgtcacaccg gccgcagcgg ccaccgcgtc gagcgacacc
     6661 gccgagaact cctgttcacg gaagatgtcc gcggccgcac cgaggatctg gccacgacgt
     6721 aggtcggggt cgagtcgggt acggggacgc ggcggcatgg cgacgacgct aaccggcgcc
     6781 cgtcactcga tgacggggca ggggttcccg gtcgcggtca gcgtgttgct gcgaccggtc
     6841 gccccgcccg aggtggcctc ggcgacgatc gtgaccgcac ccgtcgagcc gtacgggaga
     6901 tcgatggtgc cgttccatcc gttacgggtt cggttgcgca ccatcgcaac cgagccggag
     6961 gtcgtgccgg cgatccaccg cagcgtgacc gacgtcggga taccacccga ggtgatcgtg
     7021 gcggcgaacg ccgtgttggc gcacgctcct ccgccggcgg cgcgtgcgga cagggccgat
     7081 gggccgctca gccgcaacgt ggggggcgcc gccgtcgtgg tggtcgtcga tgcaggagcg
     7141 gacgtggtgg tcgtcggaac cgacgtcgtc gacgtcgacg cgaggggcac ggtcgtgggc
     7201 ggcgcgacga cccgcgtgga tgtcgggcgc agcaccgtcg tcggcgccgc ggtgtcgacg
     7261 acgagcggga cggtcgacga cggaagcggt gccgaggtgg tgacccgcgg tgtggccgtg
     7321 gtcgacgtga cgctggtcgt cgaaccgcca cgccggtccg aggtcgcaac cgtcgtggcg
     7381 tcatcggccg gcaacgccca gagtccggcg atcaccagca ccagcacgac caccgccgcg
     7441 accgcgaatc cacccggccg ccgagcccga tccggatgcg gcggaaaccc gtcgcctcgc
     7501 caccggcgtg ggcccaaccc acccgggcca ccgccgacga ccgccgtggc gagcacccga
     7561 tcgcggaccg aggtcggcgt gacga
//
LOCUS       DAOSOA010000137         7545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9759_length_7545_cov_6.416422, whole genome shotgun sequence.
ACCESSION   DAOSOA010000137 DAOSOA010000000
VERSION     DAOSOA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9759_length_7545_cov_6.416422"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKY13_09680"
     CDS             <1..475
                     /locus_tag="PKY13_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80246.1"
                     /translation="VMASVATGPGATSERVTIAAAPASTTTSTSTMPTTTSTSTTTTT
                     TTTTEPTSDHAPPAAEAAIPTESTTIGTTDPVPTPQASTPTDDLPNLWVMGPGDHLWR
                     VAEVAMTNHLGRPVANHEIDGYWRRLVALNRESLIDPDNPDLVFSGQVIELPPFL"
     gene            513..2561
                     /locus_tag="PKY13_09685"
     CDS             513..2561
                     /locus_tag="PKY13_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOT80247.1"
                     /translation="MGIVAEPTESTTVDTPILEPATRRPELDGLRAIAVLAVVVYHAD
                     PDVLPGGFVGVDLFFVLSGYLITTLLIGEHDRSGRVSLRAFWGRRARRLWPLAWCTLA
                     AIAVASATGVWGADRQRTLPAEIGAAFAQVANWYQVGHGGYVSSFAAPSPIRHFWSLA
                     IEEQFYVVWPLVLVLLLRDARFRRWAVPSGIAAIVAIAVARGWAVRPDSTATADRAYL
                     GTDVRMVALAIGAALAWAWRRTPMAGPATQRWRRVIVVWGPGGAIVLAVAAAWLHADD
                     DRLARGGFALIAIAGCGVIAATLTLPTLGAILGTRPMAALGRASYALYLTHWPLMVAL
                     GPGRSTWLRLLVGLGGGTVLAVALHHVVELPLIRRTVRPRRWIPVGTAIAAAAIIALV
                     VAVPEGPTPTEQVEASLGAVPDPVDTAPAGAPSTTASEGSDDETPTPPPSTAPPCVTA
                     APTTTVYAATGGFDPATVDEIADPTSGCGAELKVLVVGDSTGRGAANGLAALGNPLVK
                     LWDRTVLGCSFGDEDCPSWRERWAAAVAEIDPDVVLVYDNVVSDFQGVDDAPFLSDEA
                     ALVRTNQFIEATKLLSAQGAAVVFEAPAVPLRPNGLFFCDGDATNSNCDPAWLTRWNE
                     DLHAAAALTDSTVLDVRGWIAARGATTADRPDGLHLAGPALVELAAWMVPQLFAAAGR
                     "
     gene            complement(2594..3196)
                     /locus_tag="PKY13_09690"
     CDS             complement(2594..3196)
                     /locus_tag="PKY13_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOT80248.1"
                     /translation="MAVDDEDQAFKELYGRKVGERLRLIRRQKRLSLQDVEATSDQEF
                     KASVLGAYERGERAISVPRLQRLARFYRVPVDQLLPFDEDGDAPGDGADDGARRGVDT
                     DAPTKLDLARLEHLGGTEAETLSRYVRMIQVQRQDFNGRVLTIRRDDMRAIAAILGVG
                     EEHADRRLDDLGLKLGSAPVEPVDASADVPADASEPNDAP"
     gene            complement(3413..4093)
                     /locus_tag="PKY13_09695"
     CDS             complement(3413..4093)
                     /locus_tag="PKY13_09695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOT80249.1"
                     /translation="MAFVPDLDHPVLGDDDRHHLARVLRVRDGDPMVVCDGGGRWRRS
                     RFGDDAAIEEALGDVEAVPAPAPLLQVGFALIKGDRPELVVQKLTELGVDRIVPFTSD
                     HGVVRWDPKRAQRQHERFERIAREASMQCRRTHLPVLAPVTSFAELAVATPRPLLADV
                     DGAALASAAVAPDAGTVTVLIGPEGGWSAAERSAGLAAVRIGDHVLRAETASIAAATL
                     LAALRANR"
     gene            complement(4133..5263)
                     /gene="dnaJ"
                     /locus_tag="PKY13_09700"
     CDS             complement(4133..5263)
                     /gene="dnaJ"
                     /locus_tag="PKY13_09700"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT80250.1"
                     /translation="MTTDYYELLEVSQTSTADEIKRSYRRLALEFHPDKNPDNPEAEE
                     KFKELARAYETLSDPDRRARYDRFGPDESINIGDPFGNGGMGGLGDLFDAFFGGGGGG
                     SPFGGRQRGPSGPPRGPDLEAVASLSFTEAVFGAQHDVSVRTAVTCDVCSGKGAAEGS
                     SATQCPDCGGSGEVRSVRQTMLGQIVSASVCRTCSGQGEVVEDPCENCSGDGRVVTDK
                     TYTVDVPAGVDTGATLRLTGRGAVGPRGGPAGDLYVKVSVAPHDRFVRQGFDLVHDLP
                     ITMTQATLGHHIAYETLDGDEDLVIPRGTQSGRVFRLKGRGVPHLGGRGRGDLLVRAI
                     VQTPTDLRPAEAELLEQFAKLRGDEVTPADGGLLSRIRSAFK"
     gene            complement(5291..6316)
                     /gene="hrcA"
                     /locus_tag="PKY13_09705"
     CDS             complement(5291..6316)
                     /gene="hrcA"
                     /locus_tag="PKY13_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOT80251.1"
                     /translation="MLDDRKSAILRAVVQTYIETAQPVGSTHVVDATAIGVSPATVRS
                     EMTVLEREGYLTQPHTSAGRIPTEKGYRFFVDEIGPGELGPAQHRQVRGFFSHAHGEL
                     EEMLRATSRMLSKLTSTAALVVGEVADPASVRSVQLVGLGPTTYLIVVVLSSGSVIKR
                     TVERTVDVADDTLHAVGAHLTSKLMGKSLATLNSVVPSEGPPPGQHVIDADADVVLVE
                     AIEAIQAATNESGRSVHVEGASHIAGAFDASSTLREVLGLLEQQVAVVTLLRDVMDRG
                     LTVAIGSETGVTTLAECSLVVAPYMVGGEQAGTIGVLGPTRMNYQETLAAVAVVSNRL
                     SRLLSEG"
     gene            complement(6456..>7545)
                     /gene="lepA"
                     /locus_tag="PKY13_09710"
     CDS             complement(6456..>7545)
                     /gene="lepA"
                     /locus_tag="PKY13_09710"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOT80252.1"
                     /translation="EVGGRRPENTPVPALGPGEVGYLIAGIKDVGEARSGETITEAAR
                     PAAQPLDGYMEPKPMVFCGLFPVDGDAFDDLRDALEKLRLNDSSFTYEPEVSGALGFG
                     FRLGYLGLLHMEIVRERLEREFDLNLIATAPSVEYRLVKEDGTEVVVDNPADVPPPSE
                     YKHILEPYFACSIITPSNYVGTLMDLCQSRRGEMKKIDYLSPERVELQYRIPLAEVVI
                     DFFDQMKSRTQGYASLDYEPDGYDASQLVRVDILLNAEQVDAFCTIVHKDRAYDYGKK
                     MCEKLKEIIPRQQFDVPIQAAIGGRFIARQTVKAYRKDVTAKLYGGDITRKKKLLTKQ
                     KEGKKRMKNIGRVELPSDAFITALRLDD"
BASE COUNT         1098 a         2688 c         2589 g         1170 t
ORIGIN      
        1 ggtgatggcg agcgtcgcaa ccggtcccgg cgccacctcc gagcgcgtca ccatcgcggc
       61 cgctccggcg tccaccacga ccagcacgtc cacgatgccc accacgacca gcacgtccac
      121 gacgacgacg acgaccacca ccacggagcc gaccagcgac cacgctccac cggcggccga
      181 agccgcgatt cccaccgagt cgacgacgat cgggacgacc gaccccgtcc ccacgcccca
      241 ggcctcgact ccgaccgacg acctccccaa cctctgggtg atgggcccgg gcgaccacct
      301 gtggcgtgtc gccgaggtag cgatgaccaa ccacctcggg cgcccggtcg ccaaccacga
      361 gatcgacggc tactggcgcc gcctcgtggc cttgaaccgg gaatcgctca tcgaccccga
      421 caatcccgac ctcgtgttct ccggtcaggt catcgagctg ccgccgttcc tctgacgtca
      481 cccttccggc cgaccgtcgg tagcgtgccc tcatggggat cgtggccgag ccgacggagt
      541 cgaccaccgt cgacaccccg atcctcgagc cggcaacgcg ccggccggag ctggacggcc
      601 tccgagcgat cgcggtgctc gccgtcgtcg tctaccacgc cgaccccgac gtcctccccg
      661 gcgggttcgt cggggtcgac ctcttcttcg tcctctccgg ctacctcatc accacgctgc
      721 tgatcggcga acacgaccgg tcgggacggg tctcgctccg ggcgttctgg gggcgccgag
      781 cccgacggct gtggccactg gcctggtgca ccctcgccgc catcgcggtc gcctcggcca
      841 cgggcgtctg gggggccgac cgccaacgaa cgctccccgc cgagatcggc gccgcgttcg
      901 cacaggtggc caactggtac caggtcggcc acggcgggta cgtcagctcg ttcgccgcgc
      961 cgtcgccgat ccgccacttc tggagcctcg cgatcgagga gcagttctac gtcgtctggc
     1021 cactcgtact cgtcctcctc ctgcgtgacg ctcgcttccg gcggtgggcg gtgccctcgg
     1081 gcatcgcggc gatcgtcgcc atcgcggtgg cacggggctg ggcggtgcgc cccgactcga
     1141 cggctaccgc cgaccgcgcc tacctcggta ccgatgtgcg gatggtggcg ctcgcgatcg
     1201 gcgccgcgct ggcgtgggcg tggcggcgca ccccgatggc cggcccggcg acgcaacgct
     1261 ggcgacgagt gatcgtcgtg tggggccctg gtggggccat cgtgctcgcg gtcgccgcgg
     1321 cgtggctgca tgccgacgac gaccggctgg cacgcggcgg gttcgccctc atcgcgatcg
     1381 ccgggtgcgg cgtgatcgcc gccacgctga cgttgccgac actgggcgcg atcctcggta
     1441 cccgtccgat ggccgcactc ggccgggcgt cctacgcgct ctacctcacg cactggccgc
     1501 tgatggtcgc gctcggcccc ggtcgcagca cctggctgcg cctcctggtc gggctcggcg
     1561 gcggaaccgt cctcgccgtc gccctgcacc acgtcgtgga actcccgctc atccgacgca
     1621 cggtgcgacc acgtcgttgg attcccgtcg gcaccgcgat cgcagcggcc gcgatcatcg
     1681 cgctggtcgt ggccgtcccc gaggggccga ccccgaccga gcaggtcgag gcatcgctcg
     1741 gcgccgtacc cgacccggtc gacacggccc ccgccggcgc tccatccacc acggcatcgg
     1801 agggatccga cgacgagaca ccgactcctc ccccttcgac cgcgccgccg tgcgtgactg
     1861 ccgctccgac caccaccgtc tacgcggcga ccggcgggtt cgaccccgca acggtcgacg
     1921 agatcgccga tccgaccagc gggtgcggcg cggaactgaa ggtgctggtg gtcggggatt
     1981 ccaccggtcg cggcgccgcc aacgggctcg cggcgctcgg caaccccctg gtcaagctgt
     2041 gggatcgcac cgtgttgggc tgctcgttcg gcgacgagga ctgtccgagc tggcgggagc
     2101 gctgggcggc ggccgtcgcc gagatcgatc ccgatgtggt gctcgtctac gacaacgtgg
     2161 tgtcggattt ccagggcgtc gacgatgctc ccttcctctc cgacgaggcg gcgctcgttc
     2221 gcaccaacca gttcatcgag gcgacgaagc tgttgtcggc gcagggcgcg gccgtggtct
     2281 tcgaggcacc tgccgttccg ctccggccca acgggctgtt cttctgcgac ggcgacgcca
     2341 cgaactccaa ctgcgatccg gcgtggctca cccggtggaa cgaggacctc cacgccgccg
     2401 cggcgctcac ggactcgacc gtgctcgacg tgcgcggatg gatcgcggcc cgtggcgcga
     2461 ccacagccga ccgccccgac gggttgcatc tcgccgggcc ggcgctcgtc gaactcgccg
     2521 cgtggatggt gccccagttg ttcgccgccg ccggccgctg acggcgggag gccgacgacc
     2581 ggaggccgac cggctacggg gcgtcgttgg gctccgacgc atcggcgggc acatccgctg
     2641 acgcgtccac cggttcgacg ggagcgctcc cgagcttgag cccgagatcg tcgagccgcc
     2701 gatcggcgtg ctcctcgccg acacccagga tcgctgcgat cgcccgcatg tcgtcgcggc
     2761 ggatcgtgag gacgcgcccg ttgaagtcct ggcgttgcac ctggatcatg cggacgtagc
     2821 ggctgagcgt ctcggcctcg gtaccaccga ggtgctcgag gcgggcgagg tcgagcttgg
     2881 tcggagcgtc ggtgtcgacc ccgcggcggg caccgtcgtc ggcgccgtca ccgggagcgt
     2941 cgccgtcctc gtcgaacggc aggagttggt cgacgggcac gcgatagaac cgggcgagac
     3001 gctggagccg cggcaccgag atggcccgct cgccccgttc gtaggcaccg agcaccgacg
     3061 ccttgaactc ctggtcggac gtggcttcga cgtcctgcag cgacagtcgc ttctgtcgcc
     3121 ggatcaaccg gagccgctcc ccgaccttgc ggccgtacag ctccttgaac gcctggtcct
     3181 cgtcgtcgac tgccaactgg ttccccttgt ctccccagat ccgggcgcca cgatcgggct
     3241 cccgggtact ccattcggct ctcccaagcc gccggtggcc gtcgccacgc ttcgtcaccg
     3301 gctcggtgat agtagaggac gattctccgg ctcatcaaca ctctgagtca ccgtcgaacg
     3361 gcgagtccgg cccgaatctc accctccgtt gtcgtaggtc gcgaacgggc cgtcagcggt
     3421 tcgcgcgcag cgcggccaag agcgtcgctg ctgcgatcga tgccgtctcg gcgcggagca
     3481 cgtggtcgcc gatacgaacc gccgccaggc cggccgagcg ttccgccgcc gaccatccgc
     3541 cctcgggccc gatcagcacg gtgacggtgc ccgcgtcggg cgcgaccgcg gccgacgcca
     3601 gcgcggcgcc gtcgacgtcg gcaaggagcg gacgcggggt ggccaccgcc agctcggcga
     3661 aggaggtcac cggggcgagc acgggcagat gggttcgacg acattgcatc gacgcttcgc
     3721 gggcaatgcg ttcgaagcgc tcgtgctgcc gttgcgcccg tttggggtcc caccgaacca
     3781 cgccgtgatc ggacgtgaac gggacgatgc ggtcgacccc gagttcggtg agcttctgca
     3841 cgacgagctc gggtcgatca cccttgatca gggcgaaccc gacctgcagg agcggcgccg
     3901 gtgccggcac ggcctcgacg tcgccgagcg cttcctcgat cgcggcgtcg tcgccgaacc
     3961 ggctgcgtcg ccagcgaccc ccaccgtcgc acacgaccat cgggtcgccg tcgcggacgc
     4021 ggagcacccg cgccagatga tggcggtcgt cgtcgccgag cacgggatgg tcgaggtcgg
     4081 gaacgaaggc catcgggcca tcgcgatggg tcggccccgt cacgtcgtca gctcacttga
     4141 aggcgctgcg gatgcgggag aggagcccgc cgtcggcagg ggtgacctcg tcgccgcgga
     4201 gcttggcgaa ctgctcgagc aactcggcct cggccggacg cagatcggtc ggcgtctgga
     4261 cgatggcgcg gacgagcagg tcgccacgac ctcgcccgcc gagatgcggg acgccgcggc
     4321 ccttcagccg gaagacacgc cccgactggg tgccccgcgg gatcacgagg tcttcgtcgc
     4381 cgtcgagggt ctcgtaggcg atgtggtgac cgagcgtcgc ctgggtcatc gtgatcggca
     4441 ggtcgtgtac gagatcgaac ccctgtcgca cgaaccggtc gtgcggagca accgagacct
     4501 tgacgtagag gtcgcccgcc ggaccgccac gaggaccgac cgcgccgcga ccggtgagcc
     4561 gcaacgtggc gccggtgtcg acgcccgccg ggacgtcgac ggtgtacgtc ttgtcggtca
     4621 cgacccggcc gtcgcccgag cagttctcgc acgggtcctc gacgacctcg ccctgtccgc
     4681 tgcaggtgcg acacaccgac gccgagacga tctggccgag catggtctga cgcaccgagc
     4741 gaacctcgcc cgagccaccg cagtcggggc actgcgtcgc gctgctgccc tccgccgcgc
     4801 ccttgccgga acacacgtcg cacgtgaccg cggtgcgaac actcacgtcg tgctgcgcac
     4861 cgaagaccgc ctcggtgaac gagagcgagg cgaccgcctc gaggtcgggg ccgcggggcg
     4921 gacccgacgg gccgcgctgg cgaccaccga acggtgaacc gccgccgccg cctccgaaga
     4981 aggcgtcgaa cagatcgccg aggccgccca tgccaccgtt gccgaacggg tcgccgatgt
     5041 tgatcgactc gtcgggcccg aaccggtcgt agcgggcacg ccggtcgggg tcgctcaggg
     5101 tctcgtaggc gcgcgccagt tccttgaact tctcctcggc ctcggggttg tcggggttct
     5161 tgtcgggatg gaactccaac gccaggcgcc gataggaccg cttgatctca tctgccgtcg
     5221 aggtctggct gacctcgagg agctcgtaat agtcggtcgt caccgctcaa cacactcttt
     5281 ctgcacgggc tcagccttcg ctcagcagtc ggctcaggcg gttgctgacc acggccaccg
     5341 ccgccaacgt ctcctggtag ttcatgcggg tcggaccgag tacaccgatc gtgcccgcct
     5401 gctcgccgcc caccatgtag ggagcgacga ccaacgaaca ctcggcgagc gtcgtgaccc
     5461 cggtctcgga accgatcgcg accgtgaggc cgcggtccat cacgtcgcgg agcagcgtga
     5521 ccacggcgac ctgctgttcg agcaacccca ggacctcgcg caacgtcgac gacgcgtcga
     5581 acgcgccggc gatgtgggac gccccttcga cgtgcacgga tcggcccgac tcgttggtgg
     5641 ccgcctggat ggcctcgatc gcctcgacga gcaccacgtc ggcgtcggca tcgatgacat
     5701 gctgaccggg cggcggaccc tcggacggca cgaccgagtt gagggtcgcc agcgacttgc
     5761 ccatcagctt cgacgtgagg tgggcaccca ctgcgtgcag ggtgtcgtcg gccacgtcca
     5821 ccgtgcgctc gacggtgcgc ttgatgaccg agcccgagct caggaccaca acgatcaagt
     5881 aggtggtcgg gccgaggccc acgagctgca ccgaccgcac cgacgccggg tcggcgacct
     5941 cgccgacgac gagcgctgca gtcgaggtca acttcgacag catcctcgac gtggcccgca
     6001 gcatctcctc gagctcgccg tgggcgtggg agaagaagcc ccggacctgg cggtgctggg
     6061 ccggaccgag ctcaccgggt ccgatctcgt cgacgaagaa gcggtagccc ttctcggtcg
     6121 ggatgcggcc ggcggacgtg tgcggctggg tcaggtaccc ctcgcgttcg agcaccgtca
     6181 tctcactgcg cacggtggcg ggagacaccc cgatcgccgt cgcatcgacc acgtgcgtcg
     6241 agccgaccgg ctgggccgtc tcgatgtagg tctggacgac ggcacggagg attgcggatt
     6301 tccggtcgtc gagcatgatt ggcactcagt gtacgcgagt gccaaccgtc ggcgggaacg
     6361 cgtcgatcag tcgtccaggc gcacggagcc gagcgaagcg aggcgaccat caggaccgat
     6421 cgctgtcggc gaagccgaca acacaacgag ttcaatcagt cgtccaacct caacgcggtg
     6481 atgaaggcgt cggaggggag ttcgacccgg ccgatgttct tcatccgctt cttgccttcc
     6541 ttctgcttgg tgagcagctt cttcttgcga gtgatgtcgc cgccgtacag cttggcggtg
     6601 acgtccttgc ggtaggcctt gacggtctgc cgggcgatga atcgcccccc gatcgctgcc
     6661 tggatcggca cgtcgaactg ctgacggggg atgatctcct tcagcttctc gcacatcttc
     6721 ttgccgtagt cgtacgcccg gtccttgtgg acgatcgtgc agaacgcgtc gacctgctcg
     6781 gcgttgagga ggatgtcgac ccggacgagc tgcgaggcgt cgtacccgtc gggctcgtag
     6841 tcgagcgagg cgtagccctg ggtgcggctc ttcatctggt cgaagaagtc gatgacgacc
     6901 tcggcgaggg ggatgcggta ctgcagctcg actcgctcgg gcgagaggta gtcgatcttc
     6961 ttcatctcgc cgcggcgact ctggcagagg tccatgaggg tgccgacgta gttgctcggg
     7021 gtgatgatcg agcaggcgaa gtacggctcg aggatgtgct tgtactccga cgggggcgga
     7081 acgtcggccg ggttgtcgac gacgacctcg gtgccgtcct ccttgaccag gcggtactcg
     7141 accgacggtg cggtcgcgat caggttgagg tcgaactccc gctcgaggcg ttcgcgcacg
     7201 atctccatgt gcaacaggcc gaggtacccc aaccggaatc cgaagccgag cgcgcccgac
     7261 acctcgggct cgtaggtgaa cgagctgtcg ttgagccgga gtttctcgag ggcgtcgcgg
     7321 aggtcgtcga acgcgtcgcc gtcgacgggg aacagcccgc agaagaccat gggcttgggc
     7381 tccatgtagc catcgagcgg ctgggcagcg ggacgggcgg cctcggtgat ggtctcgccg
     7441 gagcgagcct cgccgacgtc cttgatgcca gcgatgagat agccgacctc gccggggccg
     7501 agcgccggga ccggggtgtt ctcgggccgc cggcccccga cctcg
//
LOCUS       DAOSOA010000138         7505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9832_length_7505_cov_6.517718, whole genome shotgun sequence.
ACCESSION   DAOSOA010000138 DAOSOA010000000
VERSION     DAOSOA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9832_length_7505_cov_6.517718"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1093)
                     /locus_tag="PKY13_09715"
     CDS             complement(107..1093)
                     /locus_tag="PKY13_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOT80253.1"
                     /translation="MSEPTIDVVGIGNALVDVISHETPDFIAGQGLVPGSMNLIDEER
                     REALYGAMGVAIEASGGSGANTTVGVASLGGTSHYLGRVRDDQLGAVFNHDIHAAGVG
                     YSTPLATDGPPTGCCLILVTEDGQRTMNTYLGASTLFGVPDVDPDVIRSGQNLYLEGY
                     LFDPPAAQEAFHYAAGIAHDAGRRVSLTLSDSFCVDRHRDAFRGLLETHVDLLFANED
                     ELCSLYEVDDVEVALKAVREDCELAAVTRSEKGSVIVTRTDRIDVPVEPVDRVVDSTG
                     AGDMYAAGFLYGLSHGRDLATCGRIASIAASEVIAHVGPRPAVTLADLIRNL"
     gene            1237..2280
                     /locus_tag="PKY13_09720"
     CDS             1237..2280
                     /locus_tag="PKY13_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT80254.1"
                     /translation="MKRTRRLLSLVALASAVSLLGAACGDDKDDASTDSGSETKSEIK
                     VGLAYDLGGRGDKSFNDAAAAGLDEAKKELGVTGKELSPSSDEDREANLKLLAEGDYN
                     PIIGVGFAYADSIKLVAEANPDVTFGTIDTVVDLPNVHNNVFAENEGSFLVGAAAGLK
                     TKSNKVGFIGGVETELIKKFEAGFVAGVKETNPTATVEVKYITPDGDFSGFNAPDKAK
                     EIAGSMYDGGADVIFHAAGGSGTGLFDAAVAAGDGKWAIGVDSDQYLGATADQQKHIL
                     TSMLKRVDVAVRDTIKAFADGDTKGKTVVLDLKADGVGYSTSGGYVDDIADQLDALKE
                     KIIAGDITVPEAP"
     gene            2373..3929
                     /locus_tag="PKY13_09725"
     CDS             2373..3929
                     /locus_tag="PKY13_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT80255.1"
                     /translation="MVDSSAAVELVGIWKRFPGVIANRDINLRVTAGTIHSLVGENGA
                     GKSTLMKTLYGMHRPDEGTISINGEEVHFRSPTDAIAYGVGMVHQHFMLADNLTVTEN
                     IILGAEPQTPRTWWKPWRTVDFAGARQELNRLKADYGIDLDLDQTVEHLGVGERQRVE
                     ILKVLFRGARILILDEPTAVLVPQEVDELFANLRSLVAEGVTVIFISHKLDEVLNISD
                     DITVIRDGTTVATVQPADVDKRRLGELMVGSELPVPDTEESTVTDEIELSVTGVSACA
                     SETDTRTVVRDVSFDIHKGEIVGIAGVEGNGQFELVEAILGLRDTTAGTVVLSGQDLA
                     GMAVHDRRHAGIGYIPFDRHREALLLAAPLWENMILGHDDSQRFSSGPWINARHAQAE
                     CDAAITRFDVATPGNGVPAYALSGGNQQKLIAGREMTAGPSLLIAAHPTRGVDVGAQA
                     AIWEELRRARAAGLAVLLISADLDELIGLSDSLLVMFRGEITAHLDPSEATPEQLGMY
                     MAGERQEVGA"
     gene            3926..5077
                     /locus_tag="PKY13_09730"
     CDS             3926..5077
                     /locus_tag="PKY13_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT80256.1"
                     /translation="MTVTEAAPAPAAPEAKASKRRPLDWGNLALSVAAPIVALLIAMG
                     ITSVLMAISGIDPGVAFSTIGKRFQESTAWIDATQAAVPLYISAIAVAIGFKMNLFNI
                     GVEGQYRVAMLGTVAIGGLVELPPVLHVTFCILVAMTIGALWAAVPAVLKVYRGVSEV
                     ISTIMLNYIATGVLAFLFSEVFAQKKAGDLNPKSKVLPTSAWMPNIVEDNLHNITGFI
                     FVAGLLGVVYYLVIWKSRFGFQLRASGANPTAARTAGINPKSMILRAMLLSGAVAGLV
                     GMPQLLGDKHAYSSDLQLQLGFDGIAVALLGRNSPAGIAAAAMTFGFLDSAARFLDLE
                     SIPKEIVTIMQGVLVLTVVVVYEIVRRIKERRTQEAASRALDASLAVAA"
     gene            5206..6360
                     /locus_tag="PKY13_09735"
                     /pseudo
     CDS             5206..6360
                     /locus_tag="PKY13_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            6360..6770
                     /locus_tag="PKY13_09740"
     CDS             6360..6770
                     /locus_tag="PKY13_09740"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOT80257.1"
                     /translation="MAPTIDWDELRDSARQMTRRSYAPYSGVHVGTAGLTDDGRVVLG
                     ANVENASYGLTLCAECSLVSHLVGTGGTRLVAICTVAGDGEPIAPCGRCRQVLFEFGG
                     ADLLVDTNAGPVTLGELLPWAFGPDDVVTRRDEP"
     gene            6785..>7505
                     /locus_tag="PKY13_09745"
     CDS             6785..>7505
                     /locus_tag="PKY13_09745"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOT80258.1"
                     /translation="MAAVTILDVIRTKRDGGELSDAQIEWFISSFTDRSIADEQASAL
                     LMAIVWRGLSTRELSTWTAAMIASGDRLDLSPVGRATCDKHSTGGVGDKVSLPLCPIV
                     AACGVAVPQLSGRGLGHTGGTLDKLETIPGWSPHMTNEQFVTQLRDIGAVIAAAGDDL
                     APADRRMYALRDVTGTVEAIPLIASSIMSKKIAEGTDALVLDVKVGSGAFLPDIGLAR
                     ELARTMVGLGSDHGVRTSALLT"
BASE COUNT         1212 a         2585 c         2512 g         1196 t
ORIGIN      
        1 gggcttggtt gtgtggtcgc gcaaccgcgc caacttgtcg tggatcagcg ggtggtcgag
       61 gaccaccagc ttcggctcgg cggttgcgtc ggtcatggcg tcccttctag aggtttcgga
      121 tcaggtcggc gagggtcacg gccgggcggg ggccgacgtg ggcgatgacc tccgacgcag
      181 cgatcgacgc gatgcgcccg cacgtcgcca gatcacgccc gtgggacaac ccgtagagga
      241 acccggcggc gtacatgtcg ccggccccgg tcgaatcgac gacccggtcg accggctcga
      301 cgggcacgtc gatgcgatcg gtgcgggtca cgatgaccga ccccttctcg ctccgcgtga
      361 cggcggccag ttcacagtcc tcacgcaccg ccttgagcgc gacctccacg tcgtcgacct
      421 cgtagagcga gcacagctcg tcctcgttgg cgaacaacag gtcgacgtgg gtctcgagaa
      481 gaccgcggaa cgcatcgcgg tgccggtcga cgcagaacga atcgctgagc gtcagcgaca
      541 cgcgccggcc ggcgtcgtgc gcgatgccgg cggcatagtg gaacgcctcc tgcgctgccg
      601 gcggatcgaa caggtagccc tccaggtaga ggttctggcc gctgcggatg acgtcggggt
      661 cgacgtcggg cacaccgaac agcgtcgatg ctccgaggta ggtgttcatg gtgcgctggc
      721 cgtcctcggt gacgaggatc aggcaacagc ccgtgggcgg accgtcggtc gcgagcggcg
      781 tcgagtagcc gacgccggcg gcgtggatgt cgtggttgaa gaccgcgccc agctgatcgt
      841 cacgtacccg accgaggtag tgcgaggtgc cgcccagcga agcgaccccg accgtggtgt
      901 tcgcacccga cccgccactc gcctcgatcg ccacgcccat ggcgccgtac agcgcctcgc
      961 gacgctcttc gtcgatgagg ttcatcgagc cgggaacgag gccttgccct gcgatgaagt
     1021 cgggggtctc gtggctgatc acgtcgacga gtgcgttgcc gatgccgacg acatcgatgg
     1081 tgggttcgct catagcccgc aaccgtaggc gccgcggggt ggccaccaca gaatcgcaac
     1141 cgggtcctcg gtcgggctgc taggaacagg tgcagtaacc cagttgccag ccccgggatg
     1201 ccggggcggc acgcacagcc tccaggaggg caccccgtga agcgaaccag aagactgtta
     1261 tcgctcgtcg cactcgcctc cgcagtcagc ctcctcggcg cggcctgcgg cgacgacaag
     1321 gacgatgcgt cgaccgactc cggcagcgag acgaagtccg agatcaaggt cggcctcgcg
     1381 tacgacctcg gcggtcgtgg cgacaagtcg ttcaacgacg ctgccgctgc cggcctcgac
     1441 gaggccaaga aggagctcgg cgtcacgggc aaggagctgt ccccctcgag cgacgaggac
     1501 cgtgaggcca acctcaagct cctcgccgag ggcgattaca acccgatcat cggtgtgggc
     1561 ttcgcgtacg ccgacagcat caagctcgtg gccgaggcca accccgacgt cacgttcggc
     1621 acgatcgaca cggtcgtgga cctgcccaac gtccacaaca acgtgttcgc cgagaacgag
     1681 ggctcgttcc tcgtcggcgc ggccgccggt ctcaagacca agagcaacaa ggtcgggttc
     1741 atcggcggcg tcgagaccga gctcatcaag aagttcgagg cgggtttcgt cgccggtgtg
     1801 aaggagacca acccgacggc caccgtcgag gtgaagtaca tcacccccga cggcgacttc
     1861 tcgggcttca acgcccctga caaggcgaag gaaatcgccg gttcgatgta cgacggcggc
     1921 gccgacgtga tcttccacgc cgctggcggc tcgggcaccg gcctcttcga cgctgcggtc
     1981 gccgcgggcg acggcaagtg ggcgatcggt gtcgactccg accagtacct cggtgcgacc
     2041 gccgaccagc agaagcacat cctcacctcg atgctcaagc gtgtcgacgt ggccgtgcgc
     2101 gacaccatca aggccttcgc cgacggcgac acgaagggca agacggtcgt cctcgacctg
     2161 aaggccgacg gcgtgggcta ctccacgtcg ggtggctacg tcgacgacat cgccgaccag
     2221 ctggacgcgc tcaaggagaa gatcatcgcc ggcgacatca cggtgcccga agcaccctga
     2281 tcgaccccgt gatcgacggg cccggtgtcg ttgcggcgac accgggcccg cgctcgtttt
     2341 agggtaggca cctcatccac gaagggacgt acgtggtcga ctcctcggca gctgtggaac
     2401 tggtcgggat ctggaaacgg ttccccggcg tcatcgccaa ccgtgacatc aacctgcggg
     2461 tgacagccgg cacgatccac tcgctcgtgg gcgagaacgg ggccggcaag tcgaccctca
     2521 tgaagacgct gtacgggatg caccgacccg atgagggcac catctcgatc aacggcgagg
     2581 aggtgcactt ccggtcgccc accgacgcga tcgcgtacgg cgtcggcatg gtgcaccagc
     2641 acttcatgct cgccgacaac ctgacggtca ccgagaacat catcctcgga gccgagcccc
     2701 agacccctcg cacgtggtgg aagccgtggc gcaccgtcga cttcgccggg gcccgccagg
     2761 aactgaaccg cctcaaggcc gactacggca tcgatctcga cctcgaccag accgtggagc
     2821 acctcggtgt cggtgagcga cagcgcgtcg agatcctcaa ggtgctgttc cgcggcgcca
     2881 ggatcctgat cctcgacgag ccgacagcgg tgctcgttcc ccaggaggtc gacgagctgt
     2941 tcgcgaacct ccgcagcctg gtcgccgagg gggtcacggt catcttcatc agccacaagc
     3001 tcgacgaggt gctcaacatc tccgacgaca tcacggtgat ccgtgacggc acgaccgtcg
     3061 cgaccgtgca gcccgccgac gtcgacaagc gtcgactcgg cgagctcatg gtgggcagcg
     3121 aactcccggt gcccgacacc gaggagtcga ccgtcaccga cgagatcgag ctgtcggtca
     3181 cgggcgtcag cgcgtgcgcc tcggagaccg acacgcgcac cgtggtccgc gacgtctcgt
     3241 tcgacatcca caagggcgag atcgtcggga tcgcaggggt cgagggcaac ggccagttcg
     3301 agctggtcga ggcgatcctc gggctacgcg acaccaccgc cggcaccgtc gtgctgagtg
     3361 ggcaggatct cgccgggatg gcggtgcacg atcgacgtca cgccggaatc gggtacatcc
     3421 ccttcgaccg acaccgtgag gcgctgctgc tcgcggcacc gctgtgggag aacatgatcc
     3481 tcggccacga cgactcgcag cgcttctcga gcggcccgtg gatcaacgct cgccacgccc
     3541 aagcggaatg cgacgcggcc atcacccggt tcgacgtcgc gacccccggc aacggggtcc
     3601 ccgcctacgc cctgtcgggt ggcaaccaac agaagctcat cgccggccgc gagatgacgg
     3661 cgggcccgag cctcttgatt gcagcgcacc cgacccgcgg cgtcgatgtc ggcgcgcaag
     3721 cggcgatctg ggaagagttg cgccgggcca gggccgccgg gctcgccgtg ttgctgatct
     3781 cggctgatct cgacgaactc atcggtctca gcgacagcct gctggtgatg ttccgtggtg
     3841 agatcacggc gcacctcgac ccgtcggagg cgacccccga acaactcggc atgtacatgg
     3901 ccggcgaacg acaggaggtc ggcgcatgac cgtgaccgag gcagctcccg cgccggcggc
     3961 cccggaagcg aaggcgtcga agcggcgccc actcgactgg ggcaatctcg cgctctcggt
     4021 cgcagccccg atcgtcgcgc tgctgatcgc catgggcatc acgtcggtac tcatggcgat
     4081 cagtggcatc gaccccggcg tcgccttctc gaccatcggc aagcggttcc aggaatcgac
     4141 ggcgtggatc gacgccaccc aggcagcggt cccgctgtac atctcggcga tcgcggtcgc
     4201 gatcgggttc aagatgaacc tgttcaacat cggggtcgaa ggtcagtacc gggtggccat
     4261 gctcggcacc gtggcgatcg gcgggctcgt cgaactcccg cccgtgttgc acgtgacctt
     4321 ctgcatcctc gtcgccatga cgatcggcgc gctgtgggcg gccgttccag cggtgctcaa
     4381 ggtgtaccgg ggtgtcagtg aggtgatctc gacgatcatg ctcaactaca tcgcgaccgg
     4441 cgtcctcgcc ttcctgttct ccgaggtgtt cgcccagaag aaggcgggcg acctcaaccc
     4501 gaagtcgaag gtgctgccga cgtcggcgtg gatgccgaac atcgtcgagg acaacctgca
     4561 caacatcacc ggcttcatct tcgtcgccgg cctcctcggt gtcgtctact acctggtgat
     4621 ctggaagagc cggttcgggt tccagctgcg ggcgtccggc gccaacccga ccgccgcacg
     4681 taccgccggc atcaacccaa agtcgatgat ccttcgggcg atgctgctgt cgggtgctgt
     4741 cgccggtctc gtcggcatgc cgcaactcct gggcgacaag cacgcgtaca gcagcgacct
     4801 gcagctccaa ctggggttcg acggcatcgc cgtcgcgctg ctcggtcgca acagcccggc
     4861 ggggatcgcc gcagcggcga tgaccttcgg cttcctcgac tcggcagccc ggttcctcga
     4921 cctcgagtcg atcccgaagg agatcgtgac catcatgcaa ggggtgctgg tgctgaccgt
     4981 cgtcgtcgtc tacgagatcg tgcgacggat caaggaacgg cgaacccagg aagcggcgtc
     5041 gagagcgctc gacgcgagcc tggcggtggc ggcatgaccg tcgccgaacc cacaccggcc
     5101 ggccaggcca cccttcccgt tccgacggag gagtccggcg gatggtgggc gtcgctgccg
     5161 aagcggcaac gctccgcgtt cctgctgatc ggtggcgtcg tcgtgatgtc ggtcgtgcgc
     5221 ctcgtcagcg gcgctgacgg gctgacgtcg tcgggcacga tcggcgccgc cctgcgcctg
     5281 gccgtcccga tcggcatggc cggcctcggc ggcatcttcg ccgaacgggt cggggtcgtg
     5341 aacatcggcc tcgagggcat gatgatcctc ggcacctggt tcggcgccta cggcgcctac
     5401 gagttcgggc cgtggtgggg tctcgccctc ggagtcgtcg gcggtgcgat cggcgggctc
     5461 ctgcacgcca tctccaccgt gacgttcaac gtcgaccaca tcatctcggg tgtcgccatc
     5521 aacatcgtcg gtgccggcct ggcgcgctac ctgtcggtcg tcgcctagga cgaaagctcc
     5581 ggcggcagcg cgacgcagtc gccgccacgc aagtccgaga tcagcacctg gaccgtgccg
     5641 ggggtgagca gcgggctcag gtcgctcgag gagaagcact ggttcctgct gagcgacgtc
     5701 gccggcatcg tccgggggtt caccaccaag atcagcccgg cgacgatcct cgctgcggtg
     5761 atcctgttgc tcgcctacct cttcctgtgg cgcacgaagt tcgggctccg cctgcgagcg
     5821 atcggcgaga acccgtacgc cgccgagtcg ctcggcgttc cggtgctgcg catcaagtac
     5881 gccggggtga tcctctccgg catgtgcgcc ggcttcggcg gcgccttcct ggcgacggtg
     5941 ctgtcgtcga tctaccggga gggccagacc agcggcaagg gcttcatcgg cctcgccacg
     6001 atgatcttcg gcaactggcg cccaggtggc ctcgccgccg gcgccctcct gttcgggtac
     6061 tccgacggct tgcagttgcg ttcgtcggcg tcggttccgg cgctgttcct gttggcggcg
     6121 atcctgtgca ccctgctgtt ggcgatcgac atccggcgtg gccacaccac cgccgcgatc
     6181 ctcgtcggcg tcgctgccgt cagcttcctc gtcgcctacc tcacgatcaa cgagctcgcc
     6241 gactcgctct cgtacctcac gccctacgtc gtgacgctcg tggtgctcgc gacctcgtcg
     6301 cagcggctcc gcccgccggc gtggggggcc gggcagtacc ggcgaggaga ggcgcactga
     6361 tggccccgac gatcgactgg gatgagttgc gcgactcggc tcggcagatg acgaggcgct
     6421 cctacgcccc gtactcgggg gtccacgtcg gcacggccgg cctcaccgac gacggccgcg
     6481 tcgtgctcgg cgcgaacgtc gagaacgcct cgtacggcct caccctctgc gccgagtgct
     6541 cgctcgtgtc gcacctcgtc gggaccggcg gcacccgact cgtcgcaatc tgcacggtgg
     6601 ccggcgacgg cgaaccgatc gccccgtgcg ggcggtgccg gcaggtgctg ttcgagttcg
     6661 gcggcgcgga cctgctggtc gacaccaacg cggggccggt cacattgggc gaactcctgc
     6721 cctgggcgtt cgggcccgac gacgtggtca cacgacgcga cgagccgtag gtacggtctg
     6781 accgatggcc gctgtcacga tcctcgacgt cattcgcacc aaacgagacg ggggtgagct
     6841 gagcgacgca cagatcgagt ggttcatcag ctcgttcacc gaccggtcga tcgccgacga
     6901 gcaggcctcc gccctgttga tggcgatcgt gtggcgaggc ctctccaccc gggagctgtc
     6961 gacatggacc gcagcgatga tcgcgtcggg cgaccgcctc gacctgtcgc cggtcggccg
     7021 tgccacctgc gacaagcact cgaccggcgg ggtcggcgac aaggtgtcgc tccccctgtg
     7081 tccgatcgtc gccgcgtgcg gcgtggcggt gccgcagctg tcgggtcgtg gcctcggcca
     7141 caccggtggc acgctcgaca agctcgagac gatccccggc tggtcgccgc acatgaccaa
     7201 cgagcagttc gtgacacagc tccgcgacat cggcgcggtg atcgccgctg ccggcgacga
     7261 cctcgccccc gccgatcggc gtatgtacgc gctgcgcgat gtcaccggca ccgtcgaggc
     7321 gatcccactc atcgccagct ccatcatgag caagaagatc gccgagggca ccgacgcgct
     7381 cgtcctcgac gtgaaggtcg gatcgggcgc gttcctaccc gacatcggtc tcgcccggga
     7441 gctggctcgc accatggtcg gtctcggatc cgaccacggc gtccgtacca gcgcactcct
     7501 caccg
//
LOCUS       DAOSOA010000139         7477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9886_length_7477_cov_4.526677, whole genome shotgun sequence.
ACCESSION   DAOSOA010000139 DAOSOA010000000
VERSION     DAOSOA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9886_length_7477_cov_4.526677"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PKY13_09750"
     CDS             <1..840
                     /locus_tag="PKY13_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT80259.1"
                     /translation="GDGNDVLEHELSAALTTLADGVTPRNVSAADVLAAAADRPGADP
                     FVRRRRATTASLAAAAVVLTVVGVVAINTDRSDQLEIGAVTGSNGPATSDEAQVTTSV
                     VPWPMEVADETLQVTGTRIPSPDPDAQSPLEHFDVADAPSVPTITGRAPDGSPLTVAP
                     GAPRLIVVVAHWCPHCQAELPALRSMLTDGTIPGAVDFTVVSTAVDPVRGNYPPSSWI
                     RAWLPDVQPLVDDRLMTAANALGVTSFPMFLAVDADGRLLARGQGQLDADQVRELVGD
                     IVK"
     gene            complement(870..1790)
                     /locus_tag="PKY13_09755"
     CDS             complement(870..1790)
                     /locus_tag="PKY13_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT80260.1"
                     /translation="MPAPEVVPLSGRRIAITGVTGQVAAPIARALAVDNEVIGLARFS
                     DATAQSSLEAAGVDCRVVDLTRGGPEASGVAAVPADVDHVLHFAVTKIGRWAKDLTAN
                     GEAVGDLMSHFRGARSFLHCSSTAVYRPTGGAPMAETDPFGDHHGAVMPTYSIAKIAG
                     ETMAQFGARHWELPTTIARLNVPYGDDDGWPVFHLEMLLAGAAIDVHSDGGRYNPIHH
                     DDIIALIPALLAVAAVPATVVNLAGPEVASIEEWSAELGRLAGGTPTFHHTDQAIPSA
                     VIDTTRQHELVGAASIGWREGMARIVAARG"
     gene            complement(1792..2625)
                     /locus_tag="PKY13_09760"
     CDS             complement(1792..2625)
                     /locus_tag="PKY13_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80261.1"
                     /translation="MRLASLAVLALVSSVSLVGCGDSGGGITISKEPAASTTAAEGDD
                     NGDSGSDDDRQAVIDYVVGVGEDSIDPEAGECVADAVLGDLSSDGLDTIRGGGDFDLT
                     EFESDDNDLLVAALDDCVDLDQALRAFSEGITSQADLPLTEDEAQCVSGGFAEEFTKA
                     GEFIAAVTAMDDDEAGARFFEALGPCMTDESAVTFLAGILSSQGQDEASAQCVAESVV
                     GTAGAQTMLGWFSEASTSGDSSELEVALSRGFLDCGVDTGDGSAPVESIPSIGGGLSG
                     N"
     assembly_gap    2700..2799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2800..4202)
                     /locus_tag="PKY13_09765"
     CDS             complement(<2800..4202)
                     /locus_tag="PKY13_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT80262.1"
                     /translation="MTDTAPAPGTTAAAPNFRELVDGVRATFDSGRTRPMQWRRRQLE
                     GLLKMMKEHESDFVDAISADLGRPVMEAFAADIGHARLQIKHVLKHFEGWAKPTKVNP
                     GVLSQPAKAEIIHEPLGVALVIAPWNYPIQLLIEPMAAALAAGNAVVAKPSELSPASA
                     AVLSRLIPQYLDNDAVVVVEGGVPETTALLEERFDHIFFTGSTAVGKVVMAAAAKHLT
                     PVTLELGGKSPTIVAADADLPVAAKRIAWGKFMNAGQTCIAPDYVLVDESVKDQFVDL
                     LTGAIGDFYGADPKATKDLGRIVNERHHDRLTGLLKDGGGTVAIGGGGDRATKFLAPT
                     VVVDPDLESGLMNEEIFGPVLPVVSVSSVDEAISFVNARPKPLALYIFSKAKETADRV
                     LERTSSGGACVNHAVVHILPDGLPFGGVGPSGMGSYHGKSGFDVFSHHKSVVRKTTRP
                     DPPLLYPPYGGWREKLVR"
     gene            4287..4784
                     /locus_tag="PKY13_09770"
     CDS             4287..4784
                     /locus_tag="PKY13_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80263.1"
                     /translation="MVDIVSRAEPDDGPEPTDETFAAFFATEYAPMVRLALLLVDDLA
                     TAEEITQDAFVQLHRRWESGNIDNPSGYLRRCVANGGRDVQRRRGVRRRARLEIPEST
                     EPAYRELDDALVRLPQRERTAVVLRYFDDLHETEIAEVLGVRPSTVRTLVRRGLNQLR
                     REVER"
     gene            4781..6760
                     /locus_tag="PKY13_09775"
     CDS             4781..6760
                     /locus_tag="PKY13_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80264.1"
                     /translation="MNPPNSPVLDEDFTGWAADLRGRVDAVDVQVPDLDDVLAEAADG
                     ATRATRATRVARPNGRVLAGIAAAVLLLVGATVVLAGRDGPSEIRTRPDRTPDRRSDP
                     LDGAVLLGPPADTVSGVQLGPDPQVLDSDEYRGDIRNWIGGQVGSPVRLLTLLTSPTG
                     STNPPFAGIAAGFIQLGDWFADGLVGTPVGATIVPIDILGAEVAFDVTLAGRLGDEDH
                     RLDLIVAQGNGQDVVLAGTGIAPDELRSLAPLLSITTDGASMVSPPLGLEPVGGNGAR
                     RSSTLYTVNGAAETVFGTRLADAAPAIAAIHRVDVLDSTSDSPTVERGGPDWALSYRT
                     TAADGDVLGIRDGMWIRIMQNSRAEPPASPLDRLAALVRYDRAEFNRLQAATPPDSGP
                     GVTDLERVVPLVDGLGSWVPTWLPDGAQVRSVASTMHSPQHAVPGLPMLWRPDTGATA
                     HFVPDPLPLGIRLDEKLVGRLRGDIYRPTTGSYLAEVVDPTVAGSPSIGIRTQGLDLE
                     QLTEVFGALERGDDGVWSMPVPPVGYEAVAGTDVEQEQPTEPDGYGLVFGRTMLWISP
                     GTGAQKASNLANFLQAVAVERSTVDGRTVYAGYDEQLGWASVFVPGDGDRPDVFVDEA
                     PWSLDDTIELALSVEQVDDAALLEFLAPLSTSRDR"
     gene            6772..7380
                     /locus_tag="PKY13_09780"
     CDS             6772..7380
                     /locus_tag="PKY13_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80265.1"
                     /translation="MADVVTLGEALALLGLAPSTTDPAVVRAAYLAAIRQAHPDVNEA
                     ANATTASARLTVAYTVITRALADGQTGPADPPTPEPDPGPDRRPAPEPVVITVDVQVI
                     ADDTIEVAAPPDLAFAWLVQTGHVVGDVTFLDRSAALLQILVSFVDEPVCQMIFDLQG
                     RAARGTTEIFCTIDSIEDRPAPPIDAVTRFVAEHLAETISAG"
BASE COUNT         1139 a         2654 c         2523 g         1061 t
ORIGIN      
        1 ggcgacggaa acgatgtgct cgaacatgaa ctgtcagcgg cgctcaccac cctcgccgac
       61 ggggtgactc cccgtaacgt cagtgccgcc gacgtcctcg cagcggccgc cgaccggccc
      121 ggcgccgatc ccttcgttcg ccggcggcga gcgaccacgg cgagcctcgc cgccgcggcc
      181 gtggtgctta ccgtggtggg ggtcgtggcg atcaacaccg accgatcgga tcagttggag
      241 atcggagccg tcaccggcag caacgggccg gccacgtccg acgaggcgca ggtcacgacg
      301 agcgtcgtgc cgtggcccat ggaggtcgcc gacgaaacgc tgcaggtcac tggcacgcgg
      361 ataccgtcgc cggaccccga tgcgcagtcc ccgctcgaac acttcgacgt cgcggacgca
      421 ccatcggtcc cgacgatcac cgggcgagca cccgatggat caccgctgac cgtcgcgcct
      481 ggagcaccgc gcctgatcgt ggtggtcgcg cactggtgcc cgcactgcca agccgagctg
      541 cccgccctgc gctcgatgct gaccgacggg acgatcccgg gcgcggtcga cttcaccgtc
      601 gtgtccaccg ctgtcgatcc cgtccggggc aactacccgc cgtcgtcgtg gatccgagcg
      661 tggctacccg atgttcagcc gctcgtcgac gaccgactca tgactgccgc caacgcgttg
      721 ggcgtcacca gcttcccgat gttcctcgcc gtcgatgccg acggccgcct cctcgcccgc
      781 ggccagggcc agctggatgc cgaccaggtc cgggagctgg tcggcgacat cgtgaagtga
      841 ccccgcccgc ccgggactca acgcacggct cagccccggg ccgccacgat ccgggccatg
      901 ccctcacgcc acccgatcga cgccgccccg accagctcgt gctggcgggt ggtgtcgatc
      961 acggcgctgg ggatcgcctg atcggtgtgg tggaacgtcg gcgtcccacc ggccaggcgg
     1021 ccgagctcgg cgctccactc ctcgatcgag gcgacctcgg gtccggcgag gttcaccact
     1081 gtcgccggca ccgccgccac ggcgaggagt gccggaatca gggcgatgat gtcgtcgtgg
     1141 tggatcgggt tgtagcggcc gccgtcgctg tggacgtcga tcgcagcgcc cgccaacagc
     1201 atctcgaggt ggaagaccgg ccagccatcg tcgtcgccgt agggcacgtt gaggcgggcg
     1261 atcgtcgtgg ggagttccca atggcgcgca ccgaactgcg ccatcgtctc cccagcgatc
     1321 ttcgcgatcg agtacgtcgg catgacggcg ccatggtggt cgccgaacgg gtcggtctct
     1381 gccatcgggg ccccgcccgt cggccggtac acggcggtcg acgagcagtg caggaacgac
     1441 cgggcgccgc ggaagtgcga catgagatcg ccgaccgcct cgccgttggc ggtgaggtcc
     1501 ttcgcccacc gaccgatctt ggtgacggcg aagtggagca cgtggtcgac gtcggccggc
     1561 accgcggcga cgccggacgc ctcgggcccg ccccgggtca ggtcgacgac acgacagtcg
     1621 acccccgctg cctcgagcga ggactgcgcc gtggcgtcgg agaaacgggc gaggccgatg
     1681 acctcattgt cgacggccag agcgcgagca atgggcgcgg cgacctgacc ggtcacgccg
     1741 gtgatggcga tgcggcgacc ggagagcggg acaacctcag gagccggcat ctcagttgcc
     1801 ggagagcccg ccgccgatcg acgggatcga ctcgacggga gcgctcccgt cgcccgtgtc
     1861 gacgccgcag tcgaggaagc cccggctgag cgccacctcg agctccgacg agtcgcccga
     1921 cgtgctcgcc tcggagaacc acccgagcat cgtctgggca ccggcggtgc cgaccaccga
     1981 ctcggcgacg cactgcgccg acgcctcgtc ctggccctgc gaactcagga tccccgcgag
     2041 gaaggtcacc gccgactcgt cggtcatgca gggaccgagc gcctcgaaga accgggcacc
     2101 cgcctcgtcg tcgtccatcg cggtgacggc ggcgatgaat tcgccggcct tcgtgaactc
     2161 ctcggcgaaa ccgccggaga cacactgcgc ctcgtcctcg gtcaacggga gatcggcctg
     2221 cgaggtgatg ccctcggaga atgcccggag ggcctggtcg aggtcgacgc aatcgtcgag
     2281 cgcagcgacg agcaggtcgt tgtcgtcgga ctcgaactcg gtcagatcga aatcgccgcc
     2341 gccgcggatc gtgtccaggc cgtccgagct cagatcgccg aggacagcgt cggcgacgca
     2401 ctcgcccgct tcgggatcga tgctgtcctc accgacgcca acgacatagt cgatgacggc
     2461 ctggcggtcg tcgtcggaac cggagtcgcc gttgtcgtcg ccctcggccg ccgtcgtgct
     2521 ggccgccggt tccttcgaaa tggtgatgcc gccgccgctg tcgccgcagc cgacgaggga
     2581 gacggaggac acgagggcca ggacggcaag tgaggcaagg cgcacgggaa ctcctgtgat
     2641 cggggaagct ctcaggctat ccggcgtctc cccggttcaa cggcccgaat cgggcgctgn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggaccagctt ctcgcgccag
     2821 ccgccgtagg gcgggtacag gaggggcgga tcggggcggg tggtcttgcg cacgaccgac
     2881 ttgtggtggc tgaacacgtc gaacccgctc ttgccgtggt acgagcccat gccagagggg
     2941 cccacgccgc cgaacggcag cccatcgggg aggatgtgca cgaccgcgtg gttgacacag
     3001 gcgccgcccg agctggtgcg ctcgagcacc cggtcggctg tctccttggc cttggagaag
     3061 atgtacagcg ccagcggctt gggccgggcg ttgacgaacg agatggcctc gtcgaccgac
     3121 gacaccgaca cgacgggcag caccggcccg aagatctcct cgttcatgag gcccgactcc
     3181 aggtcggggt cgacgacgac ggtcggggcg aggaacttgg tcgcacggtc gccgccgccg
     3241 ccgatggcga ccgtgccgcc gccgtccttc agcaggccag tgagccggtc gtgatgacgc
     3301 tcgttgacga tgcgcccgag gtccttggtc gccttgggat cggcgccgta gaagtctccg
     3361 atcgcgccgg tgagcaggtc gacgaactgg tccttcaccg actcatcgac gagcacgtag
     3421 tcaggggcga tgcaggtttg gccggcgttc atgaacttgc cccaggcgat gcgcttggcg
     3481 gcgacgggca ggtcggcgtc ggccgcgaca atcgtcgggc tcttgccgcc caactcgagc
     3541 gtgaccgggg tgaggtgctt ggctgcagcg gccatgacga ccttgccgac ggcggtcgaa
     3601 cccgtgaaga agatgtggtc gaaccgctcc tcgagcagcg cggtggtctc ggggacacca
     3661 ccctcgacca ccacgacggc gtcgttgtcg aggtactgcg ggatgaggcg ggacagcacc
     3721 gcagccgacg ccggtgacag ctccgagggc ttggcgacca ccgcattgcc ggccgcgagc
     3781 gctgctgcca tcggctcgat cagcagctgg atcgggtagt tccacggagc aatcacgagg
     3841 gcgacaccga gcggctcgtg gatgatctcg gccttcgccg gctgcgagag cacaccgggg
     3901 ttgaccttgg tcggcttcgc ccagccctcg aagtgcttga gcacgtgctt gatctgcagg
     3961 cgggcatgcc cgatgtcggc ggcgaacgcc tccatgaccg ggcgacccag gtcggcggag
     4021 atcgcgtcga cgaagtcgct ctcgtgctcc ttcatcatct tgaggaggcc ttcgagctgg
     4081 cgacgccgcc actgcatggg gcgcgtacga ccggaatcga aggtggcgcg cacgccgtcg
     4141 acgagctcac ggaagttggg agcggcggcg gtcgtaccgg gtgcaggggc ggtatcggtc
     4201 atggcgcaat ggtactcctc cgacgtcaca acggaatggc cggccgctgc gtcagtacgg
     4261 ttgtggagga catcgaggac gttgccgtgg tcgacatcgt gagccgagcc gagcccgacg
     4321 acgggcccga gccaacggac gagaccttcg cagcgttctt cgccaccgag tacgcaccga
     4381 tggtccgatt ggcgctcctg ctcgtcgacg acctggccac ggccgaggag atcacccagg
     4441 acgcgttcgt gcagctccac cggcggtggg aatcggggaa catcgacaac cccagcggct
     4501 acctccggcg atgcgtcgcc aacggcggcc gcgacgtgca acgtcggcgc ggcgtgcgga
     4561 ggcgagcccg gctcgagatt cccgagtcga ccgagccggc ctaccgcgag ctcgacgatg
     4621 cgttggtccg cctcccccag cgtgaacgca ccgcggtggt gctccgctac ttcgacgacc
     4681 tccacgagac cgagatcgcc gaagtactcg gcgtccgacc gagcaccgtg cgcaccctcg
     4741 tgcgccgcgg gctcaaccaa ctccgacgag aggtggaacg atgaatcccc ccaactcccc
     4801 cgttctcgac gaggacttca ccggctgggc cgccgacctc cggggtcggg tcgacgccgt
     4861 cgacgtgcag gtaccagacc tcgacgacgt actcgccgaa gcagccgacg gagcgacccg
     4921 agcgacccga gcgacccgcg tggcgcgccc gaacggcagg gtcctggcgg ggatcgctgc
     4981 ggccgtactc ctcctggtcg gagcgaccgt cgtgctcgcc ggacgggacg ggccgagcga
     5041 gatccgcacg cggcccgatc ggacccccga ccgacgctcg gacccgctcg acggcgcggt
     5101 cctgctcggc cccccggcgg acaccgtgtc cggtgtccag ctcggcccgg acccacaggt
     5161 gctcgactcc gacgagtacc gcggtgacat ccgcaactgg atcggcgggc aggttgggag
     5221 cccggtccga ctcctcacgc tcctcacctc accgacgggt agtaccaacc cgccgttcgc
     5281 cggcatcgca gccggcttca tccaactcgg cgactggttc gccgacgggc tcgtcggtac
     5341 acccgtcggg gcaacgatcg tgccgatcga catcctcggc gccgaggtcg cgttcgacgt
     5401 gacgttggcg ggccgactcg gcgacgagga ccaccgcctc gacctgatcg ttgcgcaggg
     5461 caatgggcag gacgtcgtgc tcgccggcac cggcatcgct cctgacgaac tccgctcctt
     5521 ggcaccactg ctctcgatca ccaccgacgg cgcgtcgatg gtgtcaccgc cgttgggcct
     5581 cgaaccggtc ggagggaacg gcgcccgccg ttcctcgacc ctgtacaccg tgaacggagc
     5641 cgcggagacc gtcttcggca cccggctcgc cgacgccgct cctgcgatcg ccgccatcca
     5701 ccgcgtcgat gtcctcgaca gcacgagcga cagcccgacc gtcgagcggg gcggccccga
     5761 ctgggcgctg tcgtaccgca ccaccgccgc tgacggcgac gtcctcggca tccgtgacgg
     5821 gatgtggatc cggatcatgc agaacagtcg ggccgaaccg ccagcctccc cgctcgaccg
     5881 tctcgccgcc ctggttcgct atgaccgggc cgagttcaac cgcctacaag ccgcgacgcc
     5941 acccgactcc ggcccggggg tcaccgacct cgaacgggtc gtaccgctcg tcgacggcct
     6001 cggtagctgg gtcccgacgt ggttgcccga cggcgcccag gtccgctcgg tcgcgtcgac
     6061 gatgcactcg ccccagcacg cggtcccggg cctgccgatg ctgtggcgcc ccgacaccgg
     6121 agcgacagca cacttcgtcc ccgacccgtt gcccctcggc atccgcctcg acgagaagtt
     6181 ggtcggtcgt ctccggggcg acatctaccg accgacgaca gggtcgtacc tggccgaggt
     6241 agtcgacccg acggtggcgg gctcgccctc catcggcatc cgaacgcagg gcctcgacct
     6301 cgagcagctc accgaggtgt tcggtgccct cgagcgcggc gacgacggtg tgtggtcgat
     6361 gccggtgcca cccgtcggct acgaggccgt ggccggcacc gacgtcgaac aggaacaacc
     6421 gaccgagccc gacgggtacg gcctcgtgtt cggacgcacg atgctgtgga tcagccccgg
     6481 caccggcgcg cagaaggcga gcaacctggc caatttcctg caagccgtcg ccgtcgagcg
     6541 gagcaccgtc gacggtcgca ccgtctatgc cgggtacgac gaacagctgg ggtgggcgtc
     6601 ggtgttcgtc cccggcgacg gcgatcgtcc cgacgtcttc gtcgacgagg cgccgtggtc
     6661 gctcgacgac accatcgagc tggccctctc ggtcgagcag gtcgacgacg ccgcactcct
     6721 cgagttcctg gccccgctct ccaccagtcg ggacaggtaa cgtgccgccc gatggcggac
     6781 gtggtgaccc tgggcgaggc gctggccctg ctcgggctcg caccgtcgac gaccgacccg
     6841 gccgtcgtcc gcgctgccta cctggcggcg atccgccagg cgcaccccga cgtcaacgag
     6901 gccgccaacg cgaccaccgc atcggcgcgg ctcaccgttg cctacaccgt gatcacccgg
     6961 gcgttggccg acgggcagac cggccccgcc gatccgccaa cacccgagcc cgacccggga
     7021 cccgatcgcc gaccggcccc cgagccggtc gtcatcaccg tcgacgtgca ggtgatcgcc
     7081 gacgacacca tcgaggtcgc ggcaccgccc gacctggcct tcgcgtggct ggtgcagacc
     7141 gggcacgtgg tcggcgacgt gacgttcctc gaccgatccg ccgcgctgct gcagatcctg
     7201 gtgtcgttcg tggacgagcc cgtctgccag atgatcttcg acctccaggg ccgggccgcc
     7261 cgcggcacga cggagatctt ctgcacgatc gattcgatcg aggaccggcc ggccccgccg
     7321 atcgacgccg tcacccgctt cgtcgccgag cacctggccg agaccatcag cgccggctga
     7381 tgccagggtg accgcgagtt ccgggcgcgc cagcggtacg ttttcgaccc gctggcgcgc
     7441 ccggaagtcg agcgagcgac ccgatcagac ctcgtcg
//
LOCUS       DAOSOA010000140         7441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_9958_length_7441_cov_4.895613, whole genome shotgun sequence.
ACCESSION   DAOSOA010000140 DAOSOA010000000
VERSION     DAOSOA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9958_length_7441_cov_4.895613"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..2517
                     /locus_tag="PKY13_09785"
     CDS             118..2517
                     /locus_tag="PKY13_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80266.1"
                     /translation="MSPQRSALHRWSWIVVPIAALVAVGIVALTGEGVAQPVSRWSDG
                     WSVIADGARGRLLVIDLKSGRVMQRIPVEASIGTALSVQEVASYAIVRVPGPRGSTLS
                     SIRLADLEVTGSRQLPPDATTVLDGDRLWQVDLEEGEAQAIDPDDLDPVGRPVRIGER
                     RHRVLPGVASVGGRVAAISAGPNPMLEIADGDSDDRRSVRLAPGSTAAIAAGDSELWL
                     AEPGRGTVTAITGSGDDRVTRIALPEAVATMFATDGRLFFPSADEGIVEVLDPVAGTI
                     TSTSTNLDWPGSAVRWDQVATTDGTLVLNQSTGDHAALLGPSGVVDVAKADDSATSDP
                     VTVPSTSAPPTTLPPSPTSTRPPTTTTPTRVNGKTTTRSTTPGSTTPGSTTPGSTTPG
                     SSTPGSTTPGATSTTTPFDPDNPTPLSTVPVTSPVSTSPSTTPTTQPGPTLVGATSPL
                     GLVAGNDFSCAVTPSGGAGCWGSNNSGQLGDGSQIDSSRPVTVSGLSDIAGLARGVGS
                     YHMCAVLGGAQAVCWGNNWDGQLGTTTATTWSTTPVVVEGLHGGVRDLTVGHGHTCAL
                     LVTGRVDCWGLNVHGQLGSGTRTQTSSTVAVTGLQGVASVVAGLEYTCALQVVGAVQC
                     WGINDVGQLGDGTTTDSAIPVPVQGLQHGVLAISAGTFSTCALLATGRVTCWGLLDWE
                     NEVISLTPTVATGVSGARQLSTGRFSTCTIGDDRLVRCWGDNDDGQLGDGGFERSASP
                     VIVAGLGPADALSAGSRQVCARRTDGEIRCWGRNEFGQVGIGSADPAAVPTPTAVASF
                     P"
     gene            2528..3817
                     /locus_tag="PKY13_09790"
     CDS             2528..3817
                     /locus_tag="PKY13_09790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT80267.1"
                     /translation="MTERYRIDELIGAGAHGSVSKAWDRRERRVVAVKLAPVASTPEI
                     DHRFLQEGRLRFDHPNVMGAIDTVRSGGRPGIVMEFGDGGDLANAVDEHGCLGEAAVL
                     LLASELLAGLDHVHSRGVMHRDIKPANVMLRSVDGRMTAALADFGATLDLDGPRLSVS
                     STQVGTIGFIAPEVMDGSDADEQSDLYSVGRTLEYALTGSTRIPVDPDEITPELGELL
                     RRLLAADPRDRPRSAATARRLIEAAVPAGAVLPGSPLPTAPPDARHRGRGRSIVTIGA
                     AAITLVVAILVAKEVLDSEDTRASGDVAADMTTTPTTPESSAADTTPPVPTSPDSSTP
                     VPTSPPPPQNLVRLVTKCGDIDVYDAVDPEFRTVSWVAPQTDAVREISLGHLDWSTQP
                     SIDIGSRLPGASPDFAVEVRCSTSGVGALIQDTTTGR"
     gene            complement(3880..5070)
                     /locus_tag="PKY13_09795"
     CDS             complement(3880..5070)
                     /locus_tag="PKY13_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80268.1"
                     /translation="MTRLVPSFDADLSRWAMTAATVPGPAGVATVSLTPGVRLEVDAL
                     DGSLLRVTVDSDDSAGLALDDLDLGVLRRFGADPGRLFGGDLAPIDLDAEACEIGGRL
                     AVLSEAARQQSFLGPRTAWWDAEANALRRMLGVADIALESSEPGAASAAAEALLEPIE
                     WGDDARSEWADTCVAPFETPRGVTRSVVSPSNAIALSDHIGDLPLGALPLGGDGEVAL
                     DTSWLPPGLVDLSRGAVVRRDRADHLLQISTDLAPGMYAAEVQQLVAVIIDETSDVIT
                     ALSSLQAGWVAGRAVATAELTVGGNRQLDDLYLLITAHPSRLPSARLRRARSATGLSR
                     AAARADRLGDEAAARRLLDDGLTRWASVDREFDRGTLRFSGTRPFLGELVESGRRVGG
                     VDLW"
     gene            complement(5067..5831)
                     /locus_tag="PKY13_09800"
     CDS             complement(5067..5831)
                     /locus_tag="PKY13_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80269.1"
                     /translation="MAWSVERFEEWFLDVIESPEAAQRCRAKAHQRGISDLGSDLLSE
                     WYLSIVGTISTWRAGGSGPPNSVSDETSAWKYILRALDNDACDLGRRTGRSPISFTTL
                     GDEQHEFDPAIEDVGGGSTGSPEFTATWSTGQDDMRRELGQLLASGRAGCRGCAGSEA
                     VAVALGVLELHDVGFLPPGDGSSDDIRGGATYWDKLVYEFLRLRDPSRVSTTAGRLSA
                     STRKWKERCGKCARELLRVVALSALPDPDERGVTGS"
     gene            complement(6113..>7441)
                     /locus_tag="PKY13_09805"
     CDS             complement(6113..>7441)
                     /locus_tag="PKY13_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOT80270.1"
                     /translation="APVPTTAPVPTTAPVPTTVPVPDPPVAVVNPSLSATPIARTPKG
                     DGYWTVEGKGKVHAFGAARFAGDANVGAGQAIGVASTRSGNGYWVFASDGGVFSFGDA
                     AFYGSMGGQRLNQPMVGAAVAPTGDGYWLVGGDGGIFTFGNLGFYGSTGGQRLNQPIV
                     GMSSTPSGRGYWLVAADGGVFSFGDAAFYGSMGGKTLNQPVVSMSATPSGRGYWLVAA
                     DGGVFAFGDAVFYGSMGGQRLNQPVYGIAPTSTGYGYWLVARDGGVFSFGDAPFLGSS
                     PTTMPPTVAGCPTGTYAELCARSVQQASSPTAARAIVFAMNQIGQPYCKPGAKAALAP
                     CNGDTNRFGDGPRRAFDCSGLVWKAYKVAGFDIVANYTGTMLTLDSTSRGRAVSSPAP
                     GDLMVNHNSTSEHVAIALADGKTVEASDWNVGVVVKSQRTFQKYIRITPN"
BASE COUNT         1209 a         2656 c         2493 g         1083 t
ORIGIN      
        1 ccgcccgcag cctcaccgcc tacgaccact gaccccttgg aaccaatcat ctaggacgga
       61 aggccgaggg cacgaaagct gtttgcaggg cccggcgggg gctgcaatgg tgaactgatg
      121 tcaccgcaac gttcggcgct gcaccggtgg agctggatcg tggtcccgat tgctgctctg
      181 gtcgcggtcg gcatcgtcgc gctcacgggc gagggagtcg cccaaccagt ctcacgctgg
      241 agcgatggat ggtcggtcat tgccgacgga gcgcgcggcc gactgctggt catcgacctc
      301 aagtccgggc gggtcatgca gagaattccg gtcgaggcgt cgatcggaac tgcgttgagc
      361 gtgcaagagg tcgcctcgta cgccatcgtt cgggtacctg gccctcgcgg ttcgacgctc
      421 tcctccatcc gactcgccga ccttgaggtc acggggtcgc gtcagctgcc gcccgacgcg
      481 acgaccgtcc tcgacggcga ccggctgtgg caggtcgacc tggaggaggg tgaggcgcaa
      541 gccatcgatc cggacgacct ggacccggtc ggtcggcccg tccggatcgg ggagcgtcgg
      601 catcgggtcc tccccggcgt cgcgagcgtg gggggccgtg tggccgccat ctcggccggc
      661 ccgaatccga tgctcgagat cgccgacggc gacagcgacg accgacgctc cgtgcggctc
      721 gcaccgggca gcacggcggc gatcgccgcg ggcgactccg agttgtggct cgccgagccc
      781 ggtaggggca ccgtgaccgc catcaccggg tccggcgacg accgggtcac ccggatcgcg
      841 ctccccgagg cggtcgccac catgttcgca accgacggtc ggctgttctt tccctcggcc
      901 gacgagggaa tcgtcgaggt cctcgatccg gtcgcgggca cgatcacctc cacatcgacg
      961 aacctcgact ggcccggaag cgcagtgcgg tgggatcagg ttgcgacgac cgacggcacc
     1021 ctggtgctca accagagcac cggcgaccat gccgcgctgc tgggtccgtc cggtgtcgtc
     1081 gacgtcgcca aagccgacga cagcgccacc agcgatccgg tcaccgtccc gtcgacgagc
     1141 gcgccgccca ccacactccc gccctccccc acgtcgaccc gtccgccgac cacgacgact
     1201 cccacacgtg tgaacgggaa gacgacgacg cggtcgacca cccctgggtc gaccacccct
     1261 ggctcgacca cgcctggctc gaccacgccc ggttccagca ccccgggttc cacgacaccc
     1321 ggtgcgacca gcaccacgac acccttcgat cccgacaacc ccacgccgct gtcgaccgtc
     1381 ccggtgacga gcccggtcag cacgagcccg tccaccacac cgaccacaca acccggcccg
     1441 acgctcgtgg gtgcgacctc gccgctcggt ctggtggccg gcaacgactt cagctgcgca
     1501 gtgaccccga gtggaggggc cggctgctgg ggcagcaaca acagcggcca gctcggcgac
     1561 ggctcgcaga tcgactcctc ccgaccggtc acggtctcgg gcctgtccga catcgcgggg
     1621 ttggcccgcg gcgtcggctc ttaccacatg tgcgcagttc tcggaggcgc acaggccgtg
     1681 tgctggggca acaactggga cggccagctc ggcaccacca ctgccacgac ctggtcgacc
     1741 acacccgtcg tcgtcgaagg cctccacggc ggggtccgag acctgaccgt aggacacggg
     1801 cacacctgtg cgttgctcgt gaccggacga gtcgactgct ggggcctcaa cgtccacggc
     1861 caactcggga gcggcaccag gacgcagacg tcgtcgaccg tcgcagtcac cggcttgcaa
     1921 ggtgtggcgt cggtcgtcgc cggtctcgaa tacacgtgcg ccctccaggt ggttggtgcc
     1981 gtgcagtgct ggggcatcaa cgacgtcggc cagctgggcg acggcacgac caccgattcg
     2041 gcgatccccg tcccagtcca ggggttgcag cacggcgtcc tggcgatctc cgccggcacc
     2101 ttctcgacgt gcgcgctgct ggcgaccggg cgggtcacgt gttggggact cctcgactgg
     2161 gagaacgagg tgatctcgct caccccaacg gtcgcgaccg gggtctcggg cgcgcgccag
     2221 ttgagcaccg gacggttctc aacgtgcacg atcggcgacg accgcctcgt ccgctgctgg
     2281 ggcgacaacg acgacggtca actcggggat ggcggcttcg aacgctcggc ctcgcccgtc
     2341 atcgtcgccg gcctcggccc cgccgacgcc ctgtcggccg gatcgaggca ggtgtgcgct
     2401 cgtcgaaccg acggtgagat ccggtgctgg ggccgcaacg aattcggcca ggtgggcatc
     2461 ggcagcgccg accccgcagc ggtccccacg cccacggccg tggcgtcgtt cccgtgagga
     2521 cgtaccggtg accgagcggt accggatcga cgagctcatc ggcgccggcg cacacgggtc
     2581 ggtatcgaag gcctgggatc gacgcgagcg acgtgtggtc gcggtcaaac tcgcgccggt
     2641 cgcgtcgacg cctgagatcg accatcgctt cctacaggag ggccgtcttc gtttcgatca
     2701 ccccaacgtc atgggcgcga tcgacaccgt gaggtcgggc ggacgccccg ggatcgtcat
     2761 ggagttcggc gacggaggcg acctggccaa cgcggtcgac gaacacgggt gcctcggcga
     2821 agccgcggtc ctgctcctcg cgtccgaact cctcgcgggc ctcgaccacg tccactcccg
     2881 aggcgtcatg caccgagaca tcaaaccggc gaacgtcatg ttgcgttcgg tcgacggccg
     2941 gatgacggca gcgctcgcag atttcggtgc gaccctcgat ctcgatggcc cacggttgtc
     3001 ggtctcgtcg acccaggtcg gcacgatcgg cttcattgct ccggaggtga tggacgggag
     3061 cgacgccgac gagcagtcag acctgtactc cgtcggccga acgctcgagt acgcactgac
     3121 ggggtcgacg cggatcccgg tcgatcccga cgaaatcacg ccggagctgg gtgagctgtt
     3181 gcgccgactc ctggcggcag atccgcgtga ccgcccgcgg agtgcggcga cggcacggcg
     3241 cctcatcgag gctgcggtcc ccgccggtgc tgtgctgccc ggttcacctc tcccgaccgc
     3301 gccacccgac gcacggcatc ggggccgggg ccggagcatc gtgacgatcg gcgctgcagc
     3361 gatcacgctg gtcgtcgcca tcctcgtagc caaggaggtc ctcgacagcg aggacacccg
     3421 ggcctccggc gatgttgctg ccgacatgac gacgacacca acgacaccgg aatcgtccgc
     3481 tgccgacacg acgccccccg ttccgacctc gcccgattcc tccacaccgg tgccaacgtc
     3541 cccgccgccg ccgcagaact tggtccgact cgtgaccaag tgcggcgaca tcgatgtcta
     3601 cgacgccgtc gaccccgagt tccggaccgt gtcgtgggtc gcaccgcaga ccgatgccgt
     3661 ccgggagatc agcctgggtc acctcgactg gagcacccag ccctcgatcg acatcggcag
     3721 tcgcctcccc ggggcgtcac ccgacttcgc ggtcgaggtg cggtgctcga cgagcggtgt
     3781 cggtgcactc atccaggata caaccacggg acggtgaccg aaggagccgg caggatcccg
     3841 ggcgtcaact ccgcgatcgg tccatcgatc ggcgccacct caccagagat cgacgccgcc
     3901 cactcggcgg ccggactcga ccagttcgcc caggaacgga cgggtgccgc tgaagcggag
     3961 ggtcccgcgg tcgaactctc ggtcgacgga cgcccaccgg gtgaggccgt cgtcgagaag
     4021 acgtcgagcg gcggcctcgt cgcccaatcg atcggcacgg gcggcagcgc gcgacagccc
     4081 tgtggcgctg cgtgccctgc ggagccgagc ggagggcagc cggctcgggt gggccgtgat
     4141 gaggaggtag agatcgtcga gttgccggtt gccgccgacg gtcagctcgg cggtcgcaac
     4201 agcccggccg gcgacccagc ccgcctggag cgaggacagg gccgtgatca cgtcggacgt
     4261 ctcgtcgatg atcaccgcga cgagttgctg cacctcggcc gcgtacatgc ccggggcgag
     4321 gtcggtcgag atctggagca gatggtcggc acggtcgcgc cgtaccacgg ccccgcgcga
     4381 gaggtcgacc aggcccggcg ggagccacga cgtatcgagc gcgacctcac cgtcgccgcc
     4441 gagcgggagc gcccccaggg gaaggtcacc gatgtggtcg gacagagcga tcgcgttgct
     4501 cggcgaaacg accgaacggg tcaccccacg cggtgtctcg aacggagcga cgcacgtgtc
     4561 ggcccattca ctgcgagcgt cgtcccccca ctcgatcggc tccagaagtg cctccgccgc
     4621 agcggacgcg gcgccgggct cgctcgactc cagggcgatg tcagccacgc cgagcatccg
     4681 tcggagcgcg ttggcctcgg cgtcccacca tgcggtccgc ggaccgagga acgactgctg
     4741 ccgagcggcc tcggacagga ctgccaaccg cccaccgatc tcgcacgctt cggcgtcgag
     4801 gtcgatcggc gccaggtcgc cgccgaacag gcgcccgggg tcggccccga atcggcggag
     4861 cacaccgagg tcgagatcgt cgagagcgag gccggcggag tcgtcggagt cgacggtgac
     4921 gcggaggagg ctgccgtcga gggcatcgac ctcgagccgc accccagggg tcagcgagac
     4981 ggtcgcgacg ccggccggac cgggcacggt ggcagctgtc atggcccacc gggacaggtc
     5041 ggcgtcgaag ctcgggacga gtctggtcat gagcccgtca ccccccgctc gtcagggtcg
     5101 gggagcgcgc tcagcgcaac gacccggagg agctcgcgtg cgcacttgcc acagcgctcc
     5161 ttccacttcc gcgtgctggc cgacaggcga ccggcggtcg tgctgacacg actgggatct
     5221 cgcagcctga ggaactcgta cacgagcttg tcccagtacg tcgcgccgcc ccggatgtcg
     5281 tcggaggagc cgtcgccggg cggcaggaac ccgacgtcgt ggagctcgag cacaccgagg
     5341 gcgactgcga cggcctcgga gccggcacaa ccgcggcatc cggctcgacc cgacgccagg
     5401 agctgcccca actcccgacg catgtcgtcc tgaccggtcg accacgtcgc cgtgaactcc
     5461 ggcgatccgg tggacccgcc gccgacgtcc tcgatcgcag gatcgaactc gtgctgctcg
     5521 tctccgagcg tagtgaaact gatcgggctc ctgcccgttc ggcggccgag atcgcacgca
     5581 tcgttgtcga gggctcggag gatgtacttc catgccgagg tctcgtccga gacggaattg
     5641 ggggggccac tccccccggc tcgccacgtc gagatcgtcc ccacgatcga caggtaccac
     5701 tcgctgagga ggtcgctccc gagatcggag attccacgct ggtgcgcctt cgccctgcag
     5761 cgttgcgctg cttcgggcga ttcgatcacg tcgaggaacc attcctcgaa acgttcgacc
     5821 gaccaagcca ccgggggatt ctgcgcagag agcgcgatcc aaatcaaccg gcaggagcgc
     5881 cgcgaacagc gcgccggcag gtcgacccga cctctccgct cgtggtgggt gatcgccgtc
     5941 gtgcgggaga gcggccccga tctccgccgg cgggtggttg ctgaccgtac atcggacatg
     6001 actttctcct gacccggtcg gcgccgtgtg gccgccggtt cgtcgtttcg tcgggcacgg
     6061 ggacacctcg ggatccgaag gagtcccgag gtgtccccgt gcgagcggat gatcagttgg
     6121 gggtgatccg gatgtacttc tggaaggtcc gttgcgactt cacgacgacg ccgacgttcc
     6181 aatccgacgc ttcgaccgtc ttgccatcgg ccagagcgat ggcgacgtgt tcgctcgtgg
     6241 agttgtggtt caccatcagg tcacctggag cgggtgaact cacggcccgt ccccgcgacg
     6301 tggagtcgag ggtgagcatg gtccccgtgt agttggccac gatgtcgaac ccggcaacct
     6361 tgtacgcctt ccagacgagt ccggagcagt cgaaggcccg tcgcggaccg tctccgaagc
     6421 ggttggtgtc gccgttgcag ggggcaagcg ccgccttcgc tcccggcttg cagtacggct
     6481 ggccgatctg gttcatggcg aacacgatgg cgcgggcagc agtcgggctg gaggcctgct
     6541 gcaccgaacg agcgcacagt tcggcgtagg tcccggtcgg gcaaccggcg acggtcggcg
     6601 gcatcgtggt cggcgaactg ccgaggaagg gggcgtcgcc gaagctgaac acaccaccgt
     6661 cgcgagcgac gagccagtag ccgtaccccg tcgacgtcgg agcgatcccg tacaccggct
     6721 gattcaaccg ctgaccaccc atggacccgt agaacaccgc gtcaccgaag gcgaacaccc
     6781 cgccatcagc agccaccagc cagtagccac gcccactcgg cgtcgcactc atcgacacca
     6841 cgggctggtt cagggtcttt cctcccatcg acccgtagaa cgccgcatcg ccgaacgaga
     6901 acaccccacc atcagccgcc accaaccagt agccacgccc actcggcgtc gaggacatcc
     6961 ccacgatcgg ctggttcagc cgctgaccac ccgtcgaccc gtagaagccg aggttcccga
     7021 acgtgaagat cccgccatcg cccccgacca gccaatagcc atcgccggtg ggagcaaccg
     7081 cagcaccgac catgggctgg ttcaaccgct gaccacccat cgacccgtag aacgctgcgt
     7141 caccgaaact gaagacccca ccatcgctgg cgaagaccca gtagccgttg ccgcttcggg
     7201 tcgatgccac cccgatcgct tgacccgcac ccacgttggc gtcgcctgcg aaccgagccg
     7261 caccgaacgc gtgcaccttg cccttcccct cgacggtcca gtacccgtcg cccttcggtg
     7321 tacgagcgat cggtgtcgcg ctcaacgacg gattcaccac cgcaaccggc ggatccggaa
     7381 ccggaaccgt ggtcgggaca ggtgccgtgg tcgggacagg tgccgtggtc gggacaggtg
     7441 c
//
LOCUS       DAOSOA010000141         7384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10081_length_7384_cov_6.610179, whole genome shotgun sequence.
ACCESSION   DAOSOA010000141 DAOSOA010000000
VERSION     DAOSOA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10081_length_7384_cov_6.610179"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKY13_09810"
     CDS             complement(<1..629)
                     /locus_tag="PKY13_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80271.1"
                     /translation="MTDRSAFAPTFVDRALERLLRAAPILSRVSQALLVLAASLFALL
                     DVGFVFAVWSNRGSDGNSLQAVTAGIGVLAVLVVVTRRRLLHTGLVTLGVASLVATAA
                     VIVARGQALPSLAALFAFALLTVSLLRSSPGRIAAGLGLLTTAAVASEALRLNQTLAA
                     SAVVVCEACFLVAVAAGLYLRWSDWRRLVGEESARTEERLEIARELHDLV"
     gene            783..1514
                     /locus_tag="PKY13_09815"
     CDS             783..1514
                     /locus_tag="PKY13_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT80272.1"
                     /translation="MISNHHLDGPGTPVLEAHSLVKRYGATTALAGVSLSISAGRSTA
                     VMGPSGSGKSTLLHCLAGFTRADEGEVLLDGHRIDRLAERASSRLRRERFGFVFQSDQ
                     LLPELPAVENTALPLMLGGTPRRRAVSEATRWFEPLGLAGMEQRRPGQLSGGQLQRVA
                     IARALVVSPSVVFADEPTAALDRATGASTMSVLTEACRQADAALLVVTHDPEVAAACD
                     RVVHMRDGQVLEIEDRVDAPTVVVR"
     assembly_gap    1528..1627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1628..3281
                     /locus_tag="PKY13_09820"
     CDS             <1628..3281
                     /locus_tag="PKY13_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80273.1"
                     /translation="SDQLGDRFGRVVGTIGDAGLARPDELVAVLGRTAAAIGPVDSQE
                     IGSSPQMRGPGVASAVTPITRYDTTQQDNDLQVYREMALVAVVLVAVPALMLVGSAAR
                     LTAARRELRLAAIRLAGATPGAVRVQAAAETALGAVAGAIVGVGASLFLAPALRGVPI
                     AGGQWFASDLRLDAARAAGLAAAAGLVSVAAAVLSLRRVASAPLGVARSAEPQRARWP
                     RLLGLGVALVGLVLSTLLARGGSSGEAVVMIAALGIVIGSLGLGGPWICSVVGRTIAR
                     FARVPSTLIAGRRIADDPKAAYRVVSSVVLAGLIAGFLAGVLPTAEAKDDRYGSTDEL
                     SISLPTADAEAVLAFDLPTRFAGTTVEQVGVGSDPAGNETVDLAITPGAGASVEQLRT
                     ITAPIRHGNPLVSSRDDLWSQATLVGDVRRGSLVVLIAGLLLAAASSAVAAAASVVDQ
                     RRTIGRLALTGVPIEMLQAARRWQTTVPLVAATTGAIAVGLGGGIALMLGFGQSSEGL
                     VPPETLQLVAAIVGAAGIGLVSAAITRPLLVNAARSGTATPE"
     gene            complement(3330..4334)
                     /locus_tag="PKY13_09825"
     CDS             complement(3330..4334)
                     /locus_tag="PKY13_09825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80274.1"
                     /translation="MRFAFTQDQLDFAEAVRDLLTNECGPERLRVAWDNDSGRIPGLW
                     DRLVEMGVVGLLAPEGVGGLALAEIDLVRIAYEAGRVALPEPLSAQAGVVVPALRDHA
                     PTADAERFLTDVCAGTSTVALGLAVNPLVVGAADASMFLLEAADGFHLVDPDVVELVP
                     EVSVDGSRRVSRVVWNPSPATLMCGAEGAVEALARAATFTAAELCGLADTMLGLTTGY
                     VTERKQFGVPIGSFQAIKHHCANALLKVEFAKPLVWRAAYSLAQGDPDAPLHASMAKA
                     AASDAADVVGRIAMQCHGGIGYTVEYDLHLYLKRAWALVAQDGSAASHRRRVADAVLG
                     "
     gene            complement(4356..5549)
                     /locus_tag="PKY13_09830"
     CDS             complement(4356..5549)
                     /locus_tag="PKY13_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80275.1"
                     /translation="MDLTWTADEQAFREECRTWLAENAPNPPLPSGDTADGFALHLEW
                     ERKLHADRWAVVSWPAEHGGRGASLWQWLIFEEEYYRAGAPQRVTQNGIFLLAPSIYE
                     FGTPAQQDYYLPRMSAGEDLWCQGWSEPNAGSDLAGITSKAVRDDEAGGWRLYGQKTW
                     TTRGAFCTHLFGLFRTDPDSVRHHGLTYLLVPLDIEGVTVRGFGRLDGDEGFAEVFFD
                     GAFVPDGDVDPAAGGVLGDVNGGWGVAMATTSSERGLTLRSPGRFTAAAARLVQLYRE
                     RGGSIDDPGVRDAVAQAWIDAEAYALYTLSDVTGIVEGRSPGARSSLNKIFWSELDVR
                     LHEAALRLLDGEAELTEWGPEGERVENPWLKGYQFSLSGPIYAGTNEIQRNIVAERVL
                     GLPRK"
     gene            5677..7140
                     /locus_tag="PKY13_09835"
     CDS             5677..7140
                     /locus_tag="PKY13_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOT80276.1"
                     /translation="MTSNTSSIHEKDTTTPQSQLAAEILAAAEPVQVLVSGLDPRNVP
                     LTWAPEVLDAFTKMEKIAASGRLLMTGRAAEAGVWEAERFATPADWLAKQQGTTAGRA
                     RADLETSQRLGGLDATADAVREGRLSPEQAGAIADAAAVNPDAERDLLARAQQDSLRR
                     TRQEAERRKAEVRSEEEKAARDKKVRDERSARFWYGNGAGHLEVRGPVAELKELEQLI
                     QREVDRTFRANRAAAQRGSRANYAFDALMAMGRGSTEAGPGAGSAKRAPLTRMTLIRV
                     DLAALMRGHVGSGELCEIGGVSVSVAELRRLLGESVLQLVLTNGEAVIDVVNLARKPT
                     VAQMLAKLFTDSCCTAEGCDRAARLEFDHRDNWARVQVTELANLDLLCDHHHDLKTRE
                     NWQLVAGTGRRAMVSPNDERHPDHLPDTTSEPVVPVLVADLEATDMPVDADAEPVARP
                     GSDARIADLAARLARIRAKDAEHRARRSGQDRLFDTA"
BASE COUNT         1100 a         2516 c         2634 g         1034 t
ORIGIN      
        1 acgaggtcgt ggagctcgcg ggcgatctcg agccgttcct cggtccgcgc cgactcctca
       61 ccgacgagtc ggcgccagtc agaccatcgg aggtacaggc cggcggcaac ggcgacgagg
      121 aagcacgcct cgcacacgac gaccgcggac gccgccaggg tctggttcaa ccgaagagct
      181 tcgctggcca ccgccgcggt cgtgaggagg ccgagtccgg cagcgatccg acccggcgac
      241 gagcgcagga gactcaccgt caggagtgcg aaggcgaaca gggcagcgag cgacggcagc
      301 gcttggcccc gggcgacgat cactgccgcg gtcgcgacga ggctcgcgac gccgagggtc
      361 acgaggccgg tgtgcaggag tcgccgacgg gtcacgacga ccagcaccgc gagcacgccg
      421 atgccagcgg tgaccgcctg cagcgagttg ccgtcagacc cacggttgga ccacaccgcg
      481 aagacgaacc cgacgtccaa caacgcgaac agcgatgcgg cgaggacgag cagggcctgg
      541 gacacccggc tcaggatcgg cgcggcccgc agcagccgtt cgagcgcgcg gtcgacgaag
      601 gtgggggcga aggccgaccg atccgtcacc ccggcatggt acgaggggac gaccaccgcc
      661 cgatatcggc caatcggctg atatcggagc acgggttcgg cgttcgaacc ggtcgaacgg
      721 ccgagccgtt cctggctgcc gggccgatgt caccgggcca tcgggtcccg atgatcgaag
      781 tcatgatctc gaaccaccac ctcgacggtc ccggcactcc ggtgctcgag gcccattccc
      841 tcgtcaagcg ctacggcgcc accaccgccc tcgccggagt gagcctctcg atttccgccg
      901 gccggtcgac cgcggtgatg gggccgagcg ggtcgggcaa gtcgacgctc ctgcactgtc
      961 tcgccgggtt cacccgtgcc gacgaaggcg aggtgctcct cgacggccac cgcatcgacc
     1021 gactcgccga gcgggcatcg tcgcggctgc gccgcgagcg cttcgggttc gtgttccagt
     1081 ccgaccaact gcttcccgag ctgcccgccg tcgagaacac ggcgctcccg ctgatgctcg
     1141 gcggcacccc acgccgccgg gccgtctccg aggccacgcg ctggttcgag ccgctcgggc
     1201 tcgccggcat ggagcagcgc cggccgggac agctgtcggg tggccagttg cagcgcgtcg
     1261 ccatcgcccg ggcactcgtc gtctcgccga gcgtggtctt cgccgacgag cccaccgccg
     1321 cgctcgaccg ggccacggga gcatcgacga tgtcggtcct caccgaggcg tgccgtcagg
     1381 ccgacgcggc gctcctggtc gtcacccacg atcccgaggt cgccgcggcc tgcgatcggg
     1441 tggtccacat gcgcgacgga caggtgctcg agatcgagga ccgagtcgac gccccgacgg
     1501 tggtggtccg atgatcgccg cactccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnngtc cgaccaactc ggggaccggt tcggccgcgt cgtcggaacg atcggtgatg
     1681 ccggcctggc ccgacccgac gagttggtgg ccgtactggg acgaacggcc gcggcgatcg
     1741 gtcccgtcga ttcgcaggag atcggcagct ccccccagat gcgcgggccc ggcgtcgcgt
     1801 cagcggtgac cccgatcacc cgatacgaca ccacacaaca ggacaacgat ctgcaggtgt
     1861 accgggagat ggccctcgtc gccgtggtac tcgtcgccgt tccggcgctc atgctggtcg
     1921 gatccgccgc ccgactgacc gcggcgcgac gggagctccg gctggccgcg atccgtctgg
     1981 ccggcgcaac gcctggagcg gtccgggtgc aggccgcggc cgagacggcg ttgggcgccg
     2041 tggccggcgc gatcgtcggc gtcggcgcgt ccctcttcct cgcgccggcg ctgcgcggcg
     2101 tgccgatcgc cggtggtcag tggttcgcgt cggaccttcg gctcgacgcc gcacgggccg
     2161 ccggtctcgc cgctgcggcc gggctggtca gcgtcgccgc cgccgtcctc tccttgcggc
     2221 gggtggcctc ggcgcccctg ggtgtcgccc gctcggccga accacaacgg gcacggtggc
     2281 cccgcctcct cgggctcggg gtcgcgctcg tcggactggt cctctccacg ctgctcgccc
     2341 gaggtggcag ctccggcgag gccgtcgtca tgatcgctgc gctcggaatc gtcatcgggt
     2401 cgttgggact gggcgggccc tggatctgct cggtggtcgg gcgcaccatt gcccgattcg
     2461 ctcgcgtccc gtcgacgctg atcgccggac gacggatcgc cgacgatccc aaggccgcct
     2521 accgggtcgt gtcgtcggtc gttctcgccg ggctcatcgc cggcttcctc gccggcgtgc
     2581 tgccgaccgc cgaggcgaag gacgatcggt acggttcgac cgacgaactc tcgatctcgc
     2641 tgccgacggc cgacgccgag gccgtgctcg cgttcgacct ccccacccgg ttcgccggaa
     2701 cgaccgtcga gcaggtcggg gtcggctcgg accccgccgg caacgagacg gtcgatctcg
     2761 caatcacccc gggagcggga gcgtcggtcg agcagctgcg cacgatcacg gcgccgatcc
     2821 ggcacggcaa tccgctggtg tcgtcgcgcg acgacctctg gagccaggcc acactcgtgg
     2881 gtgacgtccg ccgaggatcg ctggtcgtcc tcatcgccgg actcctcctc gcggcggcct
     2941 cctccgcggt ggctgcggca gcgtcggtcg tcgaccagcg ccgtaccatc gggcgcctcg
     3001 ccctcaccgg tgtcccgatc gagatgctgc aggcagcgcg gcgctggcag acgacggtac
     3061 cgctcgtcgc cgcgaccacc ggagcgatcg cggtcggtct cggcgggggg atcgcactca
     3121 tgttgggatt cggtcagtcg tccgaaggtc tggtgccacc ggagacactc cagctggtcg
     3181 ccgccatcgt cggagcggca ggaatcggac tcgtcagcgc tgccatcacc cggccgctcc
     3241 tcgtgaacgc cgcccgctcc ggcaccgcca cgcccgagtg acgaccgacc tcgcgaacga
     3301 cccgttctct gctctcgacg cgtgggaact cagccgagga cggcgtcggc gacgcgtcgc
     3361 cggtgcgatg ccgccgatcc gtcctgggcc acgagcgccc aggcccgctt gagatacagg
     3421 tgcaggtcgt actcgaccgt gtagccgatg cctccatggc actgcatcgc gatgcggccg
     3481 accacgtcag ctgcatcgct cgctgccgcc ttggccatcg aggcgtgcaa cggagcatcg
     3541 ggatcgccct gagcgagtga gtacgcggcc cgccacacga gtggcttggc gaactcgacc
     3601 ttgagcagcg cgttggcgca gtggtgcttg atcgcctgga acgaaccgat cggaacaccg
     3661 aactgcttgc gctcggtgac gtagccagtg gtcagcccga gcatggtgtc ggccagaccg
     3721 cacagctcgg cggcggtgaa cgtcgctgcc cgggcgaggg cctcgaccgc tccctcggcg
     3781 ccacacatga gcgtcgccgg cgaagggttc cagacgaccc gcgacacccg acgggaaccg
     3841 tcgacggaga cctcgggaac gagttccacg acatcggggt cgacgaggtg aaacccgtcg
     3901 gcggcttcga gcaggaacat cgacgcgtca gctgcgccga cgacgagcgg gttgacggcg
     3961 agacccagag cgaccgtgga cgtgccggca cacacgtcgg tgaggaaccg ctcggcatcg
     4021 gcggtcgggg cgtgatcgcg gagcgccggg acgacgacac ccgcctgtgc cgagagcggc
     4081 tcgggcaagg caacccggcc ggcttcgtag gcgatgcgca cgaggtcgat ctcggcgagg
     4141 gccaacccgc cgacgccctc gggagcgagg aggccgacga cgcccatctc gacgagtcgg
     4201 tcccacaggc cggggatacg gccgctgtcg ttgtcccacg cgacgcgcag ccgttcgggg
     4261 ccgcactcgt tggtgagcag gtcgcggacg gcctcggcga agtcgagctg gtcctgggtg
     4321 aaggcgaatc tcatggtcag tctctcccga accgcttact tccgcggcag accgagcaca
     4381 cgctcggcga cgatgttgcg ttggatctcg ttcgtgcccg cgtagatcgg accggacaac
     4441 gagaactggt agcccttgag ccacgggttc tcgacccgct cgccctcggg accccactcg
     4501 gtcagctcgg cctcgccgtc gagcaggcga agcgccgcct cgtgcaggcg gacgtcgagc
     4561 tccgaccaga agatcttgtt gaggctcgat cgtgcacccg gcgagcgacc ttcgacgatg
     4621 ccggtcacgt cgctcagcgt gtagagggca taggcctcgg cgtcgatcca ggcctgggcc
     4681 accgcgtcgc gaacgcccgg gtcgtcgatg gacccgccac gttcgcggta gagctgcacg
     4741 agacgcgccg cggccgcggt gaaccgaccg ggcgagcgca gcgtgaggcc gcgctcggag
     4801 gaggtggtcg ccatcgcgac accccaaccg ccattcacgt cgccgagcac accacctgca
     4861 gccgggtcga cgtcgccgtc gggaacgaac gcaccgtcga agaagacctc ggcgaagccc
     4921 tcgtcgccgt cgagtcggcc gaagccgcgg accgtgacgc cctcgatgtc gaggggcacg
     4981 aggaggtagg tgagcccgtg gtggcggacc gaatcggggt cggtgcggaa caacccgaag
     5041 aggtgggtgc aaaacgcgcc gcgggtcgtc cacgtcttct gaccgtacag ccgccagccg
     5101 ccggcctcgt cgtcgcgcac ggccttggag gtgatgcccg ccaggtcgga accggcgttc
     5161 ggttcggacc agccctgaca ccacaggtcc tcgcccgcgg acatgcgcgg aaggtagtag
     5221 tcctgctgcg ccggcgtgcc gaactcgtag atcgaggggg ccaggaggaa gatgccgttc
     5281 tgcgtcaccc gctggggtgc tcccgcccgg tagtactcct cctcgaagat cagccactgc
     5341 cagagcgacg cgccgcgacc gccgtgttcg gccggccagg agacaaccgc ccagcggtcg
     5401 gcgtgcagct tccgctccca ctcgaggtgg agggcgaacc cgtccgcggt gtcaccgctg
     5461 ggcaggggcg ggttgggggc gttctcggcg agccaggtgc ggcactcctc gcggaacgcc
     5521 tgctcgtctg ctgtccacgt cagatccacg gccacgattc tgccagcgaa tctgacgggg
     5581 tgtcagattc gtgaccactg ggtgatgctg cccaatctgc ccggcgacgg ccggatccgg
     5641 ggcctcagtg tcacgggtac gtgattgaat gatcgcatga cctcgaacac cagttcgata
     5701 cacgagaagg acacgacgac gccgcagtcg cagttggccg cggagatctt ggcagcggcc
     5761 gaaccggtcc aggtgctcgt gtcgggcctc gaccctcgga acgtgccgtt gacgtgggca
     5821 cccgaagtgc tcgatgcgtt cacgaagatg gagaagatcg cagcatcggg ccggttgttg
     5881 atgacgggtc gcgctgcgga ggcgggcgtg tgggaggcgg agcggttcgc gacaccggcg
     5941 gactggttgg cgaagcagca aggcaccaca gcggggcggg ctcgggccga tctggagacc
     6001 tcccagcgcc tgggtggcct ggacgccacc gccgacgcgg tgcgggaggg gcggttgtcg
     6061 ccggagcagg cgggggccat tgcggacgcg gcggcagtga accctgatgc cgagcgggat
     6121 ctgctggcac gggcgcagca ggattcactg cggcggacgc ggcaggaggc ggagcgccgc
     6181 aaggccgagg tgcgctcgga ggaagagaag gcggcgcggg acaagaaggt gcgtgacgag
     6241 cgcagcgccc ggttctggta cggcaacggt gccggtcatc tggaagtacg cggcccggtg
     6301 gcggagctga aggagttgga gcagttgatc caacgggagg tcgaccgcac gttccgggcg
     6361 aaccgggcgg cggcgcagcg gggatcacgg gcgaactacg cgttcgatgc gttgatggcg
     6421 atgggtcgcg gttcgacgga ggccggcccg ggggcgggtt cggcgaagcg ggcgccgttg
     6481 acccggatga cgttgatccg ggtcgacctg gccgcgttga tgcggggcca cgtcgggtcc
     6541 ggtgagctgt gtgagatcgg cggggtgtcg gtctcggtcg cggagttgcg ccgcctgttg
     6601 ggtgagtcgg tgctgcaact cgtcctcacc aatggcgagg ccgtgatcga cgtggtgaac
     6661 ctggcccgca agccgaccgt cgctcagatg ctggcgaaac tgttcaccga ctcgtgctgc
     6721 acggcggagg ggtgtgatcg ggcggcacgc ttggagttcg atcatcgtga caactgggcc
     6781 cgggtccagg tcaccgaact cgcgaacctc gatctgctgt gcgaccacca ccacgatctg
     6841 aagacccggg agaactggca gctcgttgcc gggactggcc gccgtgcgat ggtctcaccg
     6901 aacgacgagc gccaccccga tcacctgccg gacacgacgt cggagcccgt ggtgccggtc
     6961 ctggtggccg atctcgaggc gaccgacatg ccggtcgacg ccgacgccga gccggtggcc
     7021 cggccggggt ccgacgcccg gatcgccgat ctcgccgccc ggctggcccg catccgagcc
     7081 aaggacgccg agcaccgagc ccgcagatcc gggcaggacc ggctcttcga caccgcctga
     7141 gggcgcacgg cgggcgggtc gatcgagcgc atcgagccgg cgcgggcacg gcccgccgcc
     7201 gcgccgcgcc gctggacgag ttcgctctgg tcgggatggc gacggttcag gcgaggcggg
     7261 tgagcgtcgc ctcggcctcg gcgacgatgg tggagatcac ctcggcgacc gtcggcagct
     7321 cctcgatcga gccgaccacc tgcccggtcg gcaggatgcc cgcctcgacc cgaccgtcga
     7381 cgac
//
LOCUS       DAOSOA010000142         7310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10195_length_7310_cov_7.334528, whole genome shotgun sequence.
ACCESSION   DAOSOA010000142 DAOSOA010000000
VERSION     DAOSOA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10195_length_7310_cov_7.334528"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKY13_09840"
     CDS             complement(<1..674)
                     /locus_tag="PKY13_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT80277.1"
                     /translation="MTSTTNLGAPNAEPTKRRWLPLVGLLVLVVVLLVLPQTLNEFWT
                     GRITSWIPLAIAALGLNLLTGYNGQISVGHGALFGIGAYSTALILNEWKWPYLGAIVA
                     SAVICFVAGVIVGLPALRIKGLYLALVTLAVATLFPQVIEQFSDTTGGSTGLGITAPE
                     LNSRGVMAERSIRFTAPEWSGQADDQWRYYLFLVVAIVCFVLVRNLVHSRAGRSIIAI
                     RDNETAA"
     gene            complement(671..1555)
                     /locus_tag="PKY13_09845"
     CDS             complement(671..1555)
                     /locus_tag="PKY13_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT80278.1"
                     /translation="MSEFLRYLYIGLGNGAIYAMLGLGLVVIYRSTGLLNFAQGEMAM
                     FATFIVWMLHDAGLPLWVALLGGMVGGFLIGAIVHQVVVKPVGDPHQKPLAVVIVTIG
                     MFLGFNALAKLIWGSDDKTFVNLFGSGGLNLFGTEMAWQKIGALAVLGVEALLFWLLF
                     QRTKMGLAMRSVASNPESSALCGIPVNRILMVGWGLAAAIGAVAGTFTAPIGSLSSNL
                     MQLPLIYAFAAITLGGFDSFVGAIVGGLLVGILTDVVPRYVSAFEKMSLAPAFILILV
                     VLLVRPEGIFGQRKVSRV"
     gene            complement(1582..2289)
                     /locus_tag="PKY13_09850"
     CDS             complement(1582..2289)
                     /locus_tag="PKY13_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT80279.1"
                     /translation="MALLEVSGLTAGYGIVQVLFGLDFTVDAGEAVVILGANGSGKTT
                     TLRAVCGMIDSAGEVLLDGQRVTNQRPDQLLRSGVAHVPQGRGTIVDLSVEDNLRIGA
                     YTRAPKDVDADIGRWYEVFPRLGERRDQAAGSMSGGEQQMLAIARAFMSRPRLVLLDE
                     PSLGLAPIVTREVFDRVGQLVSETGTAVLLVEQNANLALEFASRAYVLEAGKIVNSGS
                     SAELHGDESIRKAYLGS"
     gene            complement(2289..3059)
                     /locus_tag="PKY13_09855"
     CDS             complement(2289..3059)
                     /locus_tag="PKY13_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT80280.1"
                     /translation="MALLEVSDIGVRFGGVVALAGLSFTIDEGQVCALIGPNGAGKTT
                     LFNVVSRLYEPSEGSVRFADVDLLGLAPHRIAPLGIARSFQNLALVPGLSVLDNVIIG
                     SHSRTTGGFWAAALWVPLLRQERRVRARAMEILDGLQLAHLAARPCAGLPYGTLKRIE
                     IARSLMAEPRLLLLDEPASGLTHGEVDELGDVILALREEHSLTVLLVEHHMGMVMRIS
                     DKVVVMELGQKIAEGTPSEVQANPRVIAAYLGEPVEEH"
     gene            complement(3200..3697)
                     /locus_tag="PKY13_09860"
     CDS             complement(3200..3697)
                     /locus_tag="PKY13_09860"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT80281.1"
                     /translation="MPMATDAAPTPGSIAARLGAAFIDFVAIALLLSLASQAWTTTSV
                     ENGVIVERPATAAYIFALGLRAAYDIVGVGRWGTTIGKRLARLGVVATDGTRAGWWRA
                     VVRFVVANAAWSLAWFAPKSWSPASGWACLVVLLAVYAPVLVDDRRRGLHDRAAGTIV
                     VATPA"
     gene            complement(3697..4461)
                     /locus_tag="PKY13_09865"
     CDS             complement(3697..4461)
                     /locus_tag="PKY13_09865"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOT80282.1"
                     /translation="MDEHRFEVICEIEPATRPDLMHVRHQVGLMSEIADTFLVPDNHI
                     GRATVSSIAVAHEVAQMGGQAIACLNARDRNVLGFRRDLLTAAAYGVDDFLFVYGDRP
                     ESGSRSDDLTVRSMIAEARAATEADGMGGPVRIGVATGLGRVPTWKLEADRLFAQVSF
                     SVDALAEWRSTVEFRGKVYAGVMVVASASMARKLSADIPQLAVPDDVVARVEADPDAG
                     VEIAARLVEDIRESGAFDGVHLVPVSRYRQLAARLA"
     gene            4882..5880
                     /locus_tag="PKY13_09870"
     CDS             4882..5880
                     /locus_tag="PKY13_09870"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT80283.1"
                     /translation="MPSSPPERLEPTPVPDGVRVMTYNLLGAQADGNVFDEWAGWAAR
                     LDQLRPDVVVLQEAQGDDVSAILGRTTTPYALASYRIWECDAKGNPEGVAILVRTSIA
                     VVGSGGTHVGESCVDPGVRRVLVWADLDVGSGPLRVYGTHLTAGGGASAASREAQIRA
                     IKRIIVAADPGDDRRWLFAGDVNVTPGSFGYRLLTGAVATEPGPYRFIDTVGDDHPNA
                     VDPVVCPTVPADPTSMATLLANPAHVRDCGYTSGWPKDDNLLGCDLLSLCTSWEQRRD
                     LSVRERIDVVLRPEGGPVTVTSTFVPNRTDADWAATGSEWFRLSDHLPYVVDVRVE"
     gene            complement(5896..6933)
                     /locus_tag="PKY13_09875"
     CDS             complement(5896..6933)
                     /locus_tag="PKY13_09875"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOT80284.1"
                     /translation="MPTGPGEAAPSSLTGAVIPTGPLAELTSGIGGAPWDVLRLDTGS
                     TLISQRDRGVIAELTPAGTLRDMATLPGPTGGGSGVLGLAVLQGTGGTPSYLYAFHTA
                     TDDNRVERAPITGTPGSYGLGPLQPILTGLPKGSFHGGGRIGFGPDGMLYVASGDAFV
                     SSNAQDLGSNAGKILRITPTGAVPADNPFPGSRVWSYGHRNIEGIGWGADGRMWASEF
                     GEDATDELNLIQPGRNYGWPLREGTTGVSDPGLTDPVATWTVAEASPAGITVVGDTVF
                     VAALRGRRLLAVDVSASPATTTSHFVNTVGRIRDVTPGPGSTIWVLTSNSDGFGTPQA
                     GDERLLSVPVG"
     gene            6935..>7310
                     /locus_tag="PKY13_09880"
     CDS             6935..>7310
                     /locus_tag="PKY13_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80285.1"
                     /translation="MAIVVVVLVLVLVVVVLVVVVLVVVLDVVLVVDAEVVLVVVEPT
                     GSAPPPSGTQPITAKVAAVNTTRRGVRVMSSSDRSACNANGTARFGRGQVNFEARAIG
                     DVNWYCGMWPRRSSTGRPRYCRS"
BASE COUNT         1157 a         2311 c         2742 g         1100 t
ORIGIN      
        1 gccgccgtct cgttgtcgcg gatggcgatg atcgatcggc cggcccggct gtggaccagg
       61 ttgcgcacga ggacgaagca cacgatggcg accacgagga acaggtagta gcgccattgg
      121 tcgtcggcct ggccgctcca ctcgggtgcg gtgaagcgga tcgaccgctc ggccatgacc
      181 ccacggctgt tgagctcggg tgcggtgatc ccgagcccgg tgctgccgcc ggtcgtgtcg
      241 gagaactgct cgatgacctg ggggaacagc gtggcgacgg ccagggtcac gagggcgagg
      301 tacagcccct tgatgcgcag ggcagggagc ccgacgatca cccccgccac gaagcagatg
      361 acggcggagg cgacgatggc accgaggtac ggccacttcc actcgttgag gatgagcgcc
      421 gtcgagtagg cgccgatgcc gaacagcgcg ccgtgaccga ccgagatctg gccgttgtat
      481 ccggtgagga ggttgagccc cagcgcggcg atggcgaggg ggatccaact ggtgatccgg
      541 ccggtccaga actcgttgag ggtctgcggc aggacgagca gcacgacgac gagtacgagc
      601 agccccacca gggggagcca ccggcgtttg gtgggttcgg cgttcggcgc gcccaggttg
      661 gtggtggagg tcatacccga ctcaccttcc gttggccgaa gatgccctct ggtcgaacga
      721 gcagcaccac gaggatcagg atgaaggctg gggccaggga catcttctcg aaggccgaca
      781 cgtagcgggg gacgacgtcg gtgaggatgc cgaccaagag gccgccgacg atggccccga
      841 cgaacgagtc gaatccgccg agcgtgattg cggcgaacgc gtagatgagg gggagctgca
      901 tcaggttcga gctgagcgaa ccgatcggcg ccgtgaatgt gccggcgacg gccccgatgg
      961 ctgcggcgag accccagccc accatgagga tgcggttgac cggtatgccg cacagtgcgg
     1021 aggactcggg gttcgaggcc accgagcgca ttgccagccc catcttcgtg cgctggaaca
     1081 gcagccagaa cagcagcgct tcgacgccga ggacggcgag cgccccgatc ttctgccacg
     1141 ccatctcggt gccgaacagg ttgaggccac cgctgccgaa gaggttgacg aaggtcttgt
     1201 cgtcggagcc ccagatgagt ttcgccaggg cgttgaagcc gaggaacatg ccgatggtca
     1261 cgatgaccac ggccagcggc ttctggtggg gatcaccgac cggtttgacc acgacctggt
     1321 ggacgatggc gccgatgagg aagcccccga ccatgccacc gaggagcgcg acccacagcg
     1381 gcagcccggc gtcgtggagc atccacacga tgaacgtggc gaacattgcc atttcgccct
     1441 gggcgaagtt gagcaggccg gtcgagcggt agatcacgac gaggccgagg ccgagcatgg
     1501 cgtagatcgc gccgttgccg aggccgatgt agaggtaacg gaggaactca gacacgggtg
     1561 ctcccgggaa tcgcagtcac atcagctccc gaggtaggcc ttgcggatcg actcgtcgcc
     1621 gtggagttcg gcggacgaac ccgagttgac gatctttccc gcctcgagga cgtacgcacg
     1681 ggaggcgaac tcgagtgcca ggttggcgtt ctgttcgacg agcaggacgg ccgtgccggt
     1741 ctcgctgacg agctggccga cacggtcgaa gacttcgcgg gtcacgatcg gggcgagacc
     1801 gagcgacggc tcgtcgagga gcacgaggcg aggccggctc atgaacgccc gagcgatcgc
     1861 gagcatctgc tgttcgccgc cgctcatcga gccggcagcc tggtcccggc gttcgccgag
     1921 ccgcgggaac acctcgtacc accggccgat gtcggcgtcg acatccttgg gtgcgcgggt
     1981 gtaggcgccg atccggaggt tgtcctcgac gctcaggtcg acgatggtgc cgcggccctg
     2041 gggaacgtgc gcgacgcccg agcggagcag ttggtcggga cgctggttcg tcacccgttg
     2101 gccgtcgagg agcacttcgc cggccgagtc gatcatgccg cagacggcgc ggagggtggt
     2161 cgtcttgccc gagccgttcg ctcccaggat gacaacggcc tcgccggcgt cgacggtgaa
     2221 gtcgagtccg aacagcacct ggacgatgcc gtagccggcg gtgaggcccg acacttcgag
     2281 gagcgccatc agtgttcctc caccggttcg ccgaggtacg cggcgatgac ccgggggttg
     2341 gcctgaacct ccgacggcgt gccttcggcg atcttctggc cgagttccat cacgaccacc
     2401 ttgtcggaga tgcgcatgac catgcccatg tggtgttcga cgagcagcac ggtgagcgag
     2461 tgctcctcgc gaagggcgag gatgacgtcg ccgagctcgt cgacctcgcc gtgggtgagc
     2521 ccgctcgccg gttcgtcgag caggaggagg cggggctccg ccatcaacga gcgggcgatc
     2581 tcgatgcgct tcagcgtgcc gtagggcagg ccggcgcagg gacgcgccgc caggtgggcg
     2641 agttggagac cgtcgaggat ctccatggcc cgggcacgga cgcggcgttc ctggcggagc
     2701 aggggaaccc acagcgccgc tgcccagaag ccgccggtgg tgcgggagtg cgatccgatg
     2761 atgacgttgt cgaggaccga caggccggga acgagcgcca ggttctggaa gctgcgggcg
     2821 atgccgaggg gcgcaatgcg gtgtggcgcg aggccgagga ggtcgacgtc ggcgaagcgg
     2881 acggagcctt cgctcggctc gtagaggcgg ctgaccacgt tgaagagcgt ggtcttgccg
     2941 gcgccgttgg gcccgatgag ggcacacacc tgaccctcgt cgatcgtgaa cgagaggccg
     3001 gccagcgcga cgactccacc gaagcggacg ccgatgtcgg agacctcgag cagcgccaac
     3061 ccatccacct tctctcgaca tggagggagc aacgtcggtc cccccgggcg ccgttcacag
     3121 tagtcgacat gttgcggaca gcaacgggca tcgtgccgtt cttctgcccg ttacttcggg
     3181 caccgatcgc gcgttcggct cacgccggcg tcgcgacgac gatcgtgccg gcggcgcggt
     3241 cgtggaggcc tcgtcgccgg tcgtccacga gcacgggcgc gtagacggcg agcaggacga
     3301 ccaggcacgc ccagcccgag gccggcgacc agctcttcgg ggcgaaccac gccagtgacc
     3361 atgcggcgtt ggcaacgacg aaccgcacca cggcgcgcca ccagccggcc cgcgttccgt
     3421 cggtggccac gacgccgagc cgggcgagtc gcttgccgat cgtggtgccc caccgaccga
     3481 ccccgacgat gtcgtaggcg gcgcgcaggc ccaacgcgaa gatgtacgca gccgtggccg
     3541 ggcgctcgac gatgaccccg ttctcgacgc tggtggtcgt ccatgcctgc gacgcgaggc
     3601 tcaacaacag cgcgatggcg acgaagtcga tgaaggcggc gccgagccgg gcggcgatcg
     3661 aaccgggcgt tggtgcggcg tcggtggcca tcggcatcag gcgagccgcg ccgccagttg
     3721 gcggtagcgg ctgaccggaa cgaggtggac gccgtcgaag gcgccggact cccggatgtc
     3781 ctcgacgagg cgggcggcga tctccacgcc ggcgtcgggg tcggcctcga ccctggcgac
     3841 gacgtcgtcg gggacggcga gctgggggat gtcggccgac agcttccggg ccatcgacgc
     3901 gctggcgacg accatgacac ctgcgtagac cttgccgcgg aactcgacgg tcgatcgcca
     3961 ctccgccaac gcgtcgacgg agaacgacac ctgggcgaac agccggtcgg cctcgagttt
     4021 ccacgtcggc acgcggccca gcccggtggc gaccccgatg cgcaccggac cgcccatccc
     4081 gtcggcctcg gtcgcggccc gggcctcggc gatcatgctg cggacggtga ggtcatcgga
     4141 cctcgatccg gattcggggc ggtcgccgta gacgaacagg aagtcgtcga ccccgtaggc
     4201 ggctgcggtg aggaggtcgc gtcggaagcc caggacgttg cggtcgcggg cgttcaaaca
     4261 ggcgatcgcc tgcccgccca tctgggcgac ttcgtgggcc acggcgatcg acgacacggt
     4321 ggcgcgaccg atgtggttgt cggggaccaa gaacgtgtcg gcgatctcgc tcatgaggcc
     4381 gacctggtgg cgaacgtgca tcaggtcggg gcgcgtcgcc ggctcgatct cgcagatcac
     4441 ctcgaaccgg tgctcatcca tggcgggaag tgtagggagc atccggccgg cgcccggatc
     4501 tcggagaatt cgagaatttt ctggaacgaa gtgtcacacc ggtgcgtcag agatccaatg
     4561 actggacgcg acgatcgatg ccggggcagg catcggcagg gatgtcgcgt gatccgagga
     4621 acaacaacat gagacgagga ctgatccgct gtcgcgggct gcggtcgacc cggctggcgt
     4681 ggtgcacgtc gatcgctcgg gaggacaccg acgactccga ggagcgctgc gatcacgcgg
     4741 tgacacggcg ccgcacctac ggttgaggcg tcgttgagga gtcgcggggc tcccacgaac
     4801 gaacggagaa cgccgatggc gcgatggtgg ggtgtgcggg cgacgatcgg ggcagggctc
     4861 acggtggtcg tagctgcttg cgtgccgtcg tctccgcccg agcggttgga gccgacgccg
     4921 gtgccggacg gggtgcgggt catgacgtac aacctgctcg gcgcccaggc cgacgggaac
     4981 gtgttcgacg agtgggcggg gtgggcggcc cgactcgacc agctccggcc cgacgtggtg
     5041 gtgctccaag aggcgcaggg cgacgacgtg tcggccatcc tcggtcgaac gacgacgccg
     5101 tacgcgctcg cgtcgtaccg gatctgggag tgcgacgcca agggcaaccc ggaaggtgtg
     5161 gcgatcctgg tgcggacgtc gatcgcggtc gtcgggagcg gtggaaccca cgtcggcgag
     5221 tcctgcgtcg atccgggggt ccgacgtgtt ctggtgtggg ccgacctcga cgtgggttcg
     5281 gggccccttc gggtgtacgg cacgcacctc acggcgggcg gcggagcgag cgcggcgtcg
     5341 cgggaggcgc agatccgggc gatcaagcgg atcatcgtcg cggccgaccc cggggatgac
     5401 cgccgctggt tgttcgccgg cgacgtcaac gtgacgcccg gaagcttcgg gtaccggctc
     5461 ctcaccggag cggtggcgac cgagccgggc ccgtaccggt tcatcgacac ggtgggtgac
     5521 gaccacccga acgctgtgga cccggtggtg tgtccgaccg ttcccgccga tcccacgtcg
     5581 atggcgaccc tgctcgcgaa cccggcgcat gtccgtgact gtgggtacac gtcgggatgg
     5641 ccgaaggacg acaacctcct cgggtgtgac ctgctgtcgc tgtgcacgtc atgggagcag
     5701 cgtcgtgacc tcagcgtccg agagcggatc gacgtcgtcc tccggccgga gggtgggccg
     5761 gtgacggtga cctcgacgtt cgtgcccaac cgcaccgacg ccgactgggc ggccacgggt
     5821 tcggagtggt ttcgactctc cgaccacctg ccgtacgtgg tcgacgtccg ggtggagtag
     5881 cggctgcccg gtcgttcagc cgaccgggac cgagagcagg cgttcgtccc ctgcctgggg
     5941 tgtgccgaaa ccgtcggagt tgctggtgag cacccagatc gtcgagcccg gcccgggggt
     6001 cacgtcgcgg atacggccga ccgtgttgac gaagtgcgag gtcgtcgttg ccggcgacgc
     6061 ggacacgtcg acagcgagga ggcgtcgccc gcgcaaggcc gccacgaaca cggtgtcgcc
     6121 gacgacggtg atccccgcgg ggctggcctc ggcgacggtc cacgtcgcaa cgggatcggt
     6181 caggccgggg tcgctgaccc cggtcgtgcc ttcgcggagt ggccatccgt agttgcggcc
     6241 cggttggatg aggttgagtt cgtcggtcgc gtcctcgccg aactcgctgg cccacatgcg
     6301 tccgtcggcg ccccagccga tcccctcgat gttgcggtgt ccgtatgacc agacgcggga
     6361 gcccgggaac gggttgtcgg cggggaccgc gccggtcggc gtgatccgga ggatcttgcc
     6421 tgcgttcgac ccgaggtctt gggcgttgga cgagacgaac gcgtcgccgg atgcgacgta
     6481 cagcattccg tcgggcccga agccgatgcg accgccgccg tggaagctgc ccttgggaag
     6541 gcccgtcagg atcggttgga gtgggccgag accgtacgac cctggggtgc ccgtgatcgg
     6601 ggcgcgctcg acgcggttgt catcggtggc cgtgtggaac gcatagagat agctgggggt
     6661 gccaccggtg ccctgcagga ccgcgaggcc gaggacgccg ctaccgccac cggtcgggcc
     6721 gggcagcgtg gccatatctc gcagcgttcc ggcgggggtg agctcggcga tcacgccgcg
     6781 gtcacgctgg ctgatgaggg tggagccggt gtcgagccgc aggacgtccc acggggctcc
     6841 gccgatcccg gacgtcagtt cggcgagcgg gccggtcggg atcacggcgc cggtgagcga
     6901 ggagggtgcc gcctcgccgg ggcccgtcgg caccgtggcg atcgtggtcg tcgtgctcgt
     6961 gctggtgctc gttgtggtgg tgctcgttgt cgtggtgctc gtcgttgtgc tcgacgttgt
     7021 gctggtggtc gacgcggagg tcgtgctggt cgtggtcgag cccaccgggt cggcgccgcc
     7081 gccgtcgggc acgcagccga tcaccgccaa ggtcgccgca gtcaacacca cgcgtcgcgg
     7141 ggtgcgtgtc atgagtagct ccgatcggtc cgcctgcaat gccaacggta ccgctcgatt
     7201 cgggcggggt caggtgaact tcgaggcccg cgcaatcggt gacgtgaact ggtattgcgg
     7261 catgtggccg cgtcgctcgt cgacggggcg gccgaggtac tgccggagcg
//
LOCUS       DAOSOA010000143         7274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10274_length_7274_cov_6.794985, whole genome shotgun sequence.
ACCESSION   DAOSOA010000143 DAOSOA010000000
VERSION     DAOSOA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10274_length_7274_cov_6.794985"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKY13_09885"
     CDS             <1..784
                     /locus_tag="PKY13_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT80286.1"
                     /translation="GITIESALVLIGGQGVLWLRSASWLVNIGWSVVNLLPVMPLDGG
                     HVVRELVEVASRRRGVTIFWLAAGLTTATLTAWFLVSPGHRFWVAIVAALMVATNIRY
                     FAITERQLKAQAIDVAHEQLMDGDLDAGIATLLPVALSHESALVSDQAYTTLAWALLH
                     QRRFVDLCQLNASRFHVQHRDLLDGATAWYRGDLYGAFNHVSTALADGRVEPPDTYFS
                     RVFGRIGEMERLAHHISMLPSDASNRAAVRLRSGLAAAGAAA"
     gene            complement(794..1522)
                     /locus_tag="PKY13_09890"
     CDS             complement(794..1522)
                     /locus_tag="PKY13_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOT80287.1"
                     /translation="MAGSELAEDAPSTTGDLGSAMARVREMRIFGAYGPKPVWRGRIH
                     QVAFFLSLPAGLWLLAGATTAAARVAVAVYWASMAGLFFTSSSYHLFAHSENAVKWWR
                     RADHSMIFVLIAGSYTPFCLLVLPRVWGIPMLVVMWVTALAGIIMKMVRVTAAGGKSG
                     SWLYVVMGWAAMIALPQLVSELGFGRMLLMFGGGILYTVGAVILGKRWLNFSPAVFGY
                     HEVWHALTVGAGVCHFTLVAMIVR"
     gene            complement(1682..2431)
                     /locus_tag="PKY13_09895"
     CDS             complement(1682..2431)
                     /locus_tag="PKY13_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80288.1"
                     /translation="MPGPRVYIHEFIDILGHNRARYMYHMTANWGPIGRAERNQLCFG
                     VWGVVGSTGRWPQVVNLWEYEDWAALGHNFEVELTGSGLQDPSLADWWAEAAEFRSGG
                     FDRILVAPDWSPSIGELTSRVGDRPPAGYAHEVVRCRPGTAGELLDAVRDGGIDAHSA
                     AGWSLVGAFRRAMADDDECILIWSFPSWASWAQFEAENDAGGGGGPAAWWRGMRSTVT
                     GRERILLADAPLSPLRTGRQPDVTDRGPVDV"
     gene            2464..5094
                     /locus_tag="PKY13_09900"
     CDS             2464..5094
                     /locus_tag="PKY13_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80289.1"
                     /translation="MSLRERLLELVVNTTEEFVGRFAPGFDLPHTFGGHLVGTDAVAD
                     VAFTLGWLGEAGVDTIAGVSIDDALRTVLARVDGPATHSFFSYRIAETLARYGTFDDN
                     RLLDGMPRAHVENLRIACDSTELLPLLDGLLPRNYLAVLARCELGRARLGILDDPAVL
                     DDLVARTADLLAGNPHGYLDESHEGAGRFDIYAADIYLFCEPLAERLGDVWQRGARNA
                     VDLVGRIATPDGNAVTWGRSTGALSIALTIELAALAAHHDLAADPVRWAALATAATAS
                     IEPWFANGVTTAHQHRAPEDYRGPQRRLQMTFDLLGKLADAAVRLPSASPDTTFAAPP
                     VDELIELSDRAAIWNVSNEQIGFVLPLVSGRSSDYLPSPRRPGLFETPVDSVLACFVP
                     IAHVAPRPLQSQGRFVPGEVPASIGRSSDGRLQVTHDSFVATLPIDGAPNGSQRLGAR
                     RDATYRLDGGSLHVEEHLTFDEAPAAVSLLIPSTVDRPLRVSADGPVRVGTTAVDGMK
                     EWQGAWSALDAVTEIEIEPSTAVSFEWSVTPKSRVSLTSPDAGYVRTVYDPISDRLAG
                     RIVERPWTSAREATDVAHIHWPEWCFGYGTSLEEHNRIAARLRRQGTRIVWTQHNLTP
                     HLDEPDVYDPIYQVWADACDLAIHHSQWGLEQVTERYRFGDHCRHEVIPHPHFGSSLT
                     ASIGLDRDDAAERLGLSTGADAPAIRIGVIGAPRKEKSLELVMDAVRRSHRDDIELVV
                     WSMSFTDVAPDDPRIVASVYENVDRDVYDLRLAACDAIALPFNGHTMLGTGTTSDLVA
                     AGIPGLVSDWPYLSEHLGGLGMAMGVGVDDWTAAIDSLTPEDLAEAAAHAPELQARYD
                     PARIGLATAIAIEALGVQRA"
     gene            5087..6061
                     /locus_tag="PKY13_09905"
     CDS             5087..6061
                     /locus_tag="PKY13_09905"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT80290.1"
                     /translation="MPEPTDTTSGTNLVERLRPNRRITGMSAILLPFTASGEVDWFGF
                     EGHVERTLAAGLAPAVNMDTGYVQSIDPATRSAVLQLTGELCGDRRFVAGAFVADAPG
                     DPFDAAGYRAAATEIVEAGGTPVVFPSWGLHELDERGWIDVQAALGADIGEFIGFELG
                     LMFVPNGRIASLDGYRELMAVPECIGAKHSSLSRRLEWDRLAIRDAERPDFMVLTGND
                     LAIDMVMWGSDYLLGLSTFAPDAFALRDRMWLEGDPSFYELNDVLQELGAFTFRHPVP
                     GYRHDAAMFLKARGWIDHDGVLGGAVVRPDSDREILVDLARRIEGWVS"
     gene            6058..>7274
                     /locus_tag="PKY13_09910"
     CDS             6058..>7274
                     /locus_tag="PKY13_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOT80291.1"
                     /translation="MTVRYPQVKKMASPAALRERTLELGLDLPVDDVVDPAGPLSSTF
                     VVRDGGAGERTVGNRFAILPMEGWDGTTGGGPTELVERRWKRFGHGGAKLVWGEATAV
                     IPDGRANPNQLVMSEGTVDDIGALRTSLVAAHRDRFGTTDDLLVGLQLTHSGRWSRPN
                     GAPEPRLAHAHPLLAGRTAPGATVFTDDELHGLVDAYVAAAVRAQRAGFDFVDVKHCH
                     GYLLHELLTAVDRPGPFGGSFDGRTRFFRTVVERIRVEAPGLAVALRLSAYDFVPFAP
                     GEGRVGRADELGTGPRPFGGDATGTGVDLTETHTMLDLCAELGVGLVSVTAGSPYYNP
                     HIQRPAYFPPSDGYLPPEDPLVGVVRMVDATRELQRRHPGIAVVTGGMSYLQDWIAHV
                     GQHLVADGSATSIG"
BASE COUNT         1137 a         2652 c         2397 g         1088 t
ORIGIN      
        1 cggcatcacg atcgagtccg cgctcgtgct gatcggcggg cagggggtgc tgtggctgcg
       61 ctcggcctcc tggctcgtca acatcggctg gagcgtcgtg aacctcctcc cggtcatgcc
      121 gctcgacggc ggccacgtgg ttcgggaact cgtcgaggtg gcgtcgcgcc ggcggggcgt
      181 caccatcttc tggctggccg ccggcctgac cacggcaaca ctcaccgcct ggttcctcgt
      241 gagccccgga caccggttct gggtagcaat cgtggctgcc ctgatggtcg ccaccaacat
      301 ccgctacttc gcgatcaccg agcgccagct caaggcgcag gcgatcgacg tcgcccacga
      361 acaactcatg gacggcgacc tcgatgccgg catcgccacc ctgctcccgg tcgcgctgtc
      421 acacgaatcg gcgctcgtgt ccgaccaggc gtacaccacc ctggcgtggg cgctcctcca
      481 ccagcgtcgc ttcgtcgacc tgtgccaact caacgcgtcg cggttccacg ttcagcatcg
      541 agacctgctg gacggggcga cggcttggta ccggggtgac ctctacggcg cgttcaacca
      601 cgtgtcgaca gcgctggccg acggccgcgt cgaacctccg gacacctact tctcccgcgt
      661 gttcggccgc atcggtgaga tggaacgcct ggcgcaccac atctcgatgt tgccgagcga
      721 tgcatcgaac cgggcagccg tccgactgcg gtcgggcctc gctgcggccg gcgccgcagc
      781 ctgacgcgac ggatcagcga acgatcatcg ccacgagggt gaagtggcag acgcccgcac
      841 cgacggtgag cgcgtgccag acctcgtggt atccgaacac cgccggcgag aagttgagcc
      901 agcgcttgcc gaggatgacg gcaccgacgg tgtagaggat gccgccgccg aacatcagca
      961 gcatgcgtcc gaatccgagt tcggagacca gctgcggcaa cgcgatcatc gcggcccagc
     1021 ccatcacgac gtagagccac gatcccgact ttccgccggc ggccgtcacg cggaccatct
     1081 tcatgatgat gccggcgagc gctgtgaccc acatgaccac gagcatgggg atgccccaca
     1141 cccgcgggag caccaacagg cagaacgggg tgtacgaccc ggcgatcagc acgaagatca
     1201 tcgagtggtc ggctctgcgc caccacttca cggcgttctc ggagtgggcg aacaggtggt
     1261 acgaggagct ggtgaagaac aggcccgcca tcgacgccca gtagacggcg acagcgaccc
     1321 gggcggcagc agtcgtcgcg ccggcaagca gccagagacc agccgggagc gagaggaaga
     1381 acgcgacctg atggatgcgg ccccgccaga cgggcttcgg gccgtaggca ccgaagatgc
     1441 gcatctcccg gacacgggcc atcgcgctgc cgagatcccc ggtggtcgac ggtgcgtcct
     1501 ctgccaactc gcttcccgcc atcatgtccc ctgccgaatc cgatccccag ccactccggc
     1561 ggtcggccgg gcgagcgaag ttgtagtgat aacacgccgt ggcgaggtcg ccaagggcat
     1621 gatcggcagg ttcgggaggg ttctgagcaa gttcatcggc acgagagcgc gatgtcaccc
     1681 atcagacgtc gacgggaccc cgatcggtca cgtccggctg gcggccggtg cgcaggggcg
     1741 agaggggggc gtcggcgagc aggatgcgtt cgcgacccgt gacggtactt cgcattcctc
     1801 gccaccacgc cgcgggtcca cccccaccgc cggcatcgtt ctcagcttcg aactgcgccc
     1861 acgaggccca ggatgggaac gaccagatca ggatgcactc gtcgtcgtcg gccatcgccc
     1921 gccggaacgc gcccaccagc gaccaccccg cggccgagtg ggcgtcgatg ccgccgtccc
     1981 gcacggcgtc gaggagctcc cccgccgtgc cgggccgaca ccggacgacc tcgtgggcgt
     2041 agccggcggg aggacggtcg cccacccggc tcgtcagctc gccgatcgag ggcgaccagt
     2101 ccggggcaac gaggattcgg tcgaacccac cgctgcggaa ctcggccgcc tcggcccacc
     2161 agtcggccag ggacggatcc tgcaggccgg agccggtcag ctccacctcg aagttgtggc
     2221 ccaacgccgc ccagtcctcg tactcccaga ggttcacgac ctgtggccag cgtcccgtcg
     2281 agccgaccac gccccacacc ccgaagcaca actggttccg ctcggcccgg ccgatcggac
     2341 cccagttcgc ggtcatgtga tacatgtacc tcgcccggtt gtggccgagg atgtcgatga
     2401 actcgtggat gtagacgcgg ggtcctggca cccggccaac ctagcgatgg gcgaaggagc
     2461 atcgtgagcc tgcgagagcg actgctggaa ctcgtcgtga acacgaccga ggagttcgtc
     2521 gggcgcttcg cccccggctt cgacctgccc cacaccttcg gtggccacct cgtcgggacc
     2581 gacgccgtcg ccgacgtggc gttcacactc ggatggctcg gcgaggccgg tgtcgacacc
     2641 atcgccggag tctcgatcga cgacgccctc cgcaccgtgc tcgcccgcgt cgacggtccc
     2701 gcgacccaca gcttcttctc gtaccggatc gccgaaacgc tggcgcgcta cggcaccttc
     2761 gacgacaacc gcttgttgga cggcatgcca cgcgcccacg tcgagaacct gcggatcgcc
     2821 tgcgacagca ccgagctgct gccgctcctc gacggtctgc tgcctcgcaa ctacctggcg
     2881 gtgctcgccc gctgcgaact cggccgcgcc cgcctcggga tcctcgacga cccggccgtg
     2941 ctcgacgacc tcgtcgcacg gaccgccgac ctgctcgccg gcaaccccca cggctacctc
     3001 gacgagtccc acgagggcgc cggacggttc gacatctacg cggcggacat ctacctgttc
     3061 tgcgagccgt tggctgaacg cctcggcgac gtctggcaac gcggggcgcg caacgccgtc
     3121 gacctcgtcg gcaggattgc cacacctgac gggaacgcgg tcacctgggg tcgatcgacg
     3181 ggggcgctgt cgattgcgct cacgatcgag ctcgcggccc tggccgccca ccacgacctc
     3241 gccgccgacc cggtgcggtg ggcagcgctc gcgacggcgg cgaccgcgtc gatcgaaccg
     3301 tggttcgcca acggcgtgac caccgcgcac cagcaccggg cacccgagga ctatcgagga
     3361 cctcagcggc gactgcagat gacgttcgac ctgctcggca aattggcgga cgctgccgtc
     3421 cggctcccga gcgcgtcgcc ggacacgacg ttcgcggccc cgccggtgga cgagctgatc
     3481 gagctgtcgg atcgggccgc gatctggaac gtctcgaacg agcagatcgg gttcgtgctc
     3541 ccgctcgtgt ccggccgatc cagcgactac ctgccctccc cccgccgacc gggactcttc
     3601 gagacaccag tggactcggt gctcgcgtgc ttcgtcccca tcgcccacgt cgcgccgcgg
     3661 ccgctccaga gtcagggccg gtttgttccg ggcgaggttc cggcgtcgat cggacgcagc
     3721 agcgacggtc ggctccaggt gacccatgac tcattcgtcg cgacgctgcc catcgacggc
     3781 gcacccaacg gttcccagcg cttgggggca cgacgcgacg cgacctaccg actggacggg
     3841 gggtcgctcc acgtcgagga acacctgacg ttcgacgagg ctccggctgc ggtcagcctg
     3901 ctcatcccat cgacagtcga ccggccgctc cgcgtcagcg ccgacggacc ggtgcgcgtc
     3961 ggcaccactg ccgtcgacgg catgaaggaa tggcagggag cgtggagcgc gctggatgcc
     4021 gtcaccgaga tcgagatcga accgtcgacc gccgtctcgt tcgagtggag cgtgacgccc
     4081 aagtcgcggg tgagcctgac atcgcccgac gccggctacg tgcgcaccgt gtacgacccg
     4141 atctcagatc gactcgctgg acgcatcgtc gagcgaccgt ggacgtcggc acgcgaggcc
     4201 accgacgtcg cccacatcca ctggcccgaa tggtgcttcg gctacgggac cagcctcgag
     4261 gagcacaacc gcatcgcggc ccggttgcgg cggcaaggga cgcggatcgt gtggacgcag
     4321 cacaacctca ccccgcacct cgacgagccc gacgtctacg acccgatcta ccaggtgtgg
     4381 gccgatgcct gtgacctcgc gatccaccac tcgcagtggg ggctcgagca ggtcaccgag
     4441 cgctaccggt tcggcgacca ctgccgccat gaggtcatcc cccaccctca cttcgggtcg
     4501 tcgctcaccg cgtccatcgg cctcgatcgc gacgacgccg ccgagcgcct cggtctctcc
     4561 accggcgccg acgctccggc gattcgcatc ggtgtgatcg gggcgcctcg caaggagaag
     4621 tcgctggagc tcgtgatgga cgcggtccgc cggagccacc gcgacgacat cgagctggtg
     4681 gtctggtcca tgtcgttcac cgacgtcgcg cccgacgacc cccgcatcgt ggcctcggtg
     4741 tacgagaacg tcgaccgcga cgtctacgac ctccgcctcg ccgcctgcga tgcgatcgcc
     4801 ctccccttca acggccacac catgctcgga accggcacga cctccgacct cgtcgccgcc
     4861 ggaatccccg gtctcgtgtc ggactggccc tacctctccg agcacctcgg cggcctcggc
     4921 atggcgatgg gtgtcggcgt cgacgactgg accgcggcga tcgactccct cactcctgaa
     4981 gacctcgccg aggcagcggc gcacgccccc gagctgcagg ctcgctacga tccggcgcgc
     5041 atcggcctgg caaccgccat cgccatcgaa gccctgggag tgcaacgtgc ctgagccgac
     5101 cgacacgacg tccggcacga acctggtcga acgcctccgg cccaaccgcc gcatcaccgg
     5161 gatgtcggcg atcctcctgc cgttcacggc gagcggcgag gtggattggt tcggcttcga
     5221 gggccacgtc gaacgcaccc tggcagccgg actcgcgccg gccgtcaaca tggacaccgg
     5281 ctacgtgcag tcgatcgatc ccgccacccg cagcgcggtg ctccagctca ccggcgagct
     5341 gtgtggcgac cgtcggttcg tcgccggtgc cttcgtcgcc gatgcacccg gcgacccgtt
     5401 cgacgccgcc ggctaccggg ccgcggcgac cgagatcgtc gaggcgggcg ggactccggt
     5461 cgtgttcccg tcgtgggggc tccacgagct cgacgaacgc ggatggatcg acgtccaagc
     5521 ggccctcgga gccgacatcg gtgagttcat cgggttcgag ctggggctca tgttcgtgcc
     5581 gaacggccgc atcgcgtcac tcgacgggta tcgagagctg atggccgtgc cggagtgcat
     5641 cggcgcgaag cactcgtcgc tctcccgtcg cctcgagtgg gaccggctcg cgatccgcga
     5701 tgccgagcgt cccgacttca tggtgctcac gggcaacgac ctcgccatcg acatggtgat
     5761 gtggggctcg gactacctcc tcggcctgtc gacgttcgcg cccgacgcgt tcgccctgcg
     5821 ggatcgcatg tggctcgagg gtgacccgtc gttctacgag ctgaacgacg tgctccagga
     5881 gttgggggcc ttcaccttcc ggcacccggt ccctggctac cgccacgacg cggcgatgtt
     5941 cctgaaggct cgcggctgga tcgaccacga cggcgtgctc ggcggggccg tcgtccggcc
     6001 ggacagcgac cgcgagatcc tcgtcgacct cgcccgacgc atcgaggggt gggtctcgtg
     6061 accgtccggt acccccaggt caagaagatg gcgtcgcccg cggcgctccg ggagcgcacg
     6121 ctcgaactgg gtctcgacct accggtcgac gatgtcgtcg atccggctgg tccgctgtcc
     6181 tcgacgttcg tcgtgcgcga cgggggcgcc ggcgagcgca cggtcggcaa ccggttcgcg
     6241 atcctcccga tggaagggtg ggacggcacg accggcggtg gaccgaccga gctggtggag
     6301 cggcgatgga agcgcttcgg tcacggtggc gccaagctcg tctggggcga ggccacggcc
     6361 gtgattcccg acggtcgagc caaccccaac caactggtga tgtcggaagg aaccgtcgac
     6421 gacatcggcg cgttgcgaac ctcgctggtc gccgcccacc gagaccggtt cggcaccacc
     6481 gacgacctgc tcgtcggcct ccagctcacg cactccggtc ggtggtcccg ccccaacggt
     6541 gcacccgaac cacgattggc tcacgcccac ccgctgctcg ccggacggac cgctccgggt
     6601 gcgaccgtgt tcaccgatga cgaactgcac ggcctggtcg acgcgtacgt cgcggccgca
     6661 gtgcgggcac agcgagccgg cttcgacttc gtcgacgtga agcactgcca cggatacctc
     6721 ctgcacgaac tgctgacggc ggtcgatcgc cccggcccgt tcggtgggtc gttcgacggg
     6781 cgcacgcgtt tcttccgcac cgtcgtcgaa cgcatccgcg tcgaggcccc gggcctggcg
     6841 gtcgcgttgc gactgtcggc ctacgacttc gtgccgttcg ctcccggtga ggggcgcgtg
     6901 ggcagagcgg acgaactcgg gacggggcca cgaccgttcg ggggcgacgc caccgggact
     6961 ggggtcgacc tgaccgagac ccacaccatg ctcgacctgt gcgccgaact cggcgtcggc
     7021 ctggtgtcgg tgaccgccgg cagcccgtac tacaacccgc acatccagcg gcccgcctac
     7081 ttcccaccca gcgacggtta cctcccaccc gaggatccgt tggtcggcgt ggttcgcatg
     7141 gtcgacgcca cccgcgagct gcaacggcga caccccggaa tcgccgtggt gaccggcggc
     7201 atgtcgtacc tgcaggactg gatcgcgcac gtgggccaac acctcgtcgc cgacgggtcg
     7261 gcgacgtcga tcgg
//
LOCUS       DAOSOA010000144         7241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10334_length_7241_cov_6.181464, whole genome shotgun sequence.
ACCESSION   DAOSOA010000144 DAOSOA010000000
VERSION     DAOSOA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10334_length_7241_cov_6.181464"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..921
                     /gene="truA"
                     /locus_tag="PKY13_09915"
     CDS             139..921
                     /gene="truA"
                     /locus_tag="PKY13_09915"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT80292.1"
                     /translation="MDIELNEESRQRVRLLVAYDGSAFSGFARNLGVTTVAGELEAHL
                     RRVLKHEVVITGAGRTDKGVHAWGQVVTFDTFADRLQPERLQRSINSVCGPAIAVRAI
                     DLVDTDFDARFSAKWRRYRYTILTAATPNPFLYRYAWHVTDPISVDAMDEAGHAILGE
                     HDFSSFCRRPPDRHDGTPASLVREVTGLRWERLEGDLLRCEITARAFCHQMVRSIVGT
                     LVDVGRGRLATDQVATILAARDRNAAGDLAPPQGLCLHEVGY"
     gene            1113..1562
                     /gene="rplM"
                     /locus_tag="PKY13_09920"
     CDS             1113..1562
                     /gene="rplM"
                     /locus_tag="PKY13_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT80293.1"
                     /translation="MTTYSPKASEITREWHVVDAEGMVLGRLATEVARILRGKHKPIF
                     SPHMDTGDHVIIVNADKVVLTKNKAADKMVYSHSGYPGGIKSSTYAQDLDRKPADAVR
                     RTVRGMLPKNRLGRQQLTKLKVYAGPTHPHQAQHPQTLVVEGARARS"
     gene            1609..2001
                     /gene="rpsI"
                     /locus_tag="PKY13_09925"
     CDS             1609..2001
                     /gene="rpsI"
                     /locus_tag="PKY13_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT80294.1"
                     /translation="MPHPLVQSTGRRKRAVARVRFRPGTGTITINKRPLEDYFPNDTH
                     RMVLSEPLKVTSTTESYDIDATIHGGGVSGQAGAMRLGISRALIALDEELRPTLKKAG
                     FLTRDSREKESKKYGLKKARKAPQYSKR"
     assembly_gap    2251..2350
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2351..3157
                     /locus_tag="PKY13_09930"
     CDS             <2351..3157
                     /locus_tag="PKY13_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT80295.1"
                     /translation="VLDCGNGASSDVASTVFTRLGAEVVALHVTPDGSNINDASGSTH
                     PESLRTAVVAEGAQLGFAFDGDADRMLAVDHQGRLIDGDQLIAVAAVDLKRRGLLVND
                     TVVVTVMSNLGFRIGMAAEGIEVVDTAVGDRYVLEALDQGGHVLGGEQSGHIIFRDRA
                     TTGDGVLSAVVIAEIVARTGRPLADLADAAMTRLPQVLRNVRIEAPMPDVTVRIADEL
                     TAAAAALGADGRVLVRPSGTEPVVRVMVEAVDAVTAQEWCDHLVQVVESL"
     gene            3370..6735
                     /locus_tag="PKY13_09935"
     CDS             3370..6735
                     /locus_tag="PKY13_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT80296.1"
                     /translation="MIDRVAGGAPSIEQLEQTTQLVAAVNLLLRPATGMAALLGNPEL
                     SFEVETVIDALNAEILEIDGVLDAGFAGADLELEQLNAAMIDLKDAVWSVARDRLRAA
                     REVAALSNGDVSPAGAIALFSLHQALSAIDRLEVRGRDSAGIQLVVHRHDVDLGDPAV
                     AAEIARRNDVNFSSGAIRVEGDSVVFVYKAAFEIGELGDNTAALRAAVRSDALLQRLL
                     GAPSVEALVLGHTRWASVGIISEPNAHPQSSDELDLADGPLWTAVLNGDVDNHADIIA
                     AESMTIAPAVTTDAKVIPALVSRRRAGGLAAHDAFRDSVASMDGSVAIGASQAGDPES
                     LFLALRGSGQALYVGLADDAFIVASEPYGVVEETSRFLRMDGETPANPENPTASRGQI
                     VQLTATGAGTVDGIRRWSYDGTELVVTEDELAHAEITTRDIDRGSFPHFLLKEITDAP
                     VSFRKTLRGKLLERDGVLSVELGELTLPTSVRERIADGSIGRVMVIGQGTAAAAGASL
                     ALALGDLVADTTVRVEAVLATELSGFRLRADMSDTLIVAVSQSGTTTDTNRTVDLVRG
                     RGATVIAIVNRRNSDLCDKSDGVLYTSDGRDVEMSVASTKAFYAQIAAGLVLATAIAD
                     LVPGAVHSSSSGQQQLLAALRELPDRMAETLARRDQIATAAHQFGPPRRYWAIVGNGP
                     NQIAAREIRIKLSELCYKSIACDATEDKKHIDLSAEPMILVCAAGLIGSTAADVGKEV
                     AIYRAHKAAPIVIATDGENPFDAALSVISVPATHERLAFILSAMAGHLFGYEAALAID
                     AQARPLREARSVIESLVTDHADELRTGEGAVELLAGTARSLQPLAAQFMDGLRSGVYN
                     GHLEAATAVKVSSLFRYATGTSSLDSYQSEFGKVGTPGVVLDDLTTGLTAAIEELTRP
                     VDAIKHQAKTVTVGISRSDESLLQVPLVLGVLEAGAPRDRLSYATLRSLAELDPAVED
                     VIGYTRYRVENGEGDDATAVVVDRGGISTGLSSRTDRDPRLRGTKHLVAREHELMVAK
                     GRGDGRTVLIVPETKDSQTTGLTLLHLRLRDRLPAATARGVLSGYRRRFQALRDAVTE
                     TEDVFRDDLLAEQDTLDLLCEPILDLADRWRSA"
     gene            complement(6990..>7241)
                     /locus_tag="PKY13_09940"
     CDS             complement(6990..>7241)
                     /locus_tag="PKY13_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80297.1"
                     /translation="ASSVIHDGVVSELSDREMAVLKVLGKGSVVSRAELIRDAGLSEL
                     SARRCESLIMSLRQILGAETIINLRRRGWRLAADVELRP"
BASE COUNT         1134 a         2545 c         2370 g         1092 t
ORIGIN      
        1 caacgcgccg atggcgcgca tcgaacttgt ctaactccct gggccaaggc caccttcgct
       61 aggcacggtc gcttcgctcc ctgctatcgc tccggtacct tggccgatcc tggcaacgaa
      121 cgggtagatc ggcgagtcat ggacatcgaa ttgaacgagg agtcgcgcca acgggtgcgg
      181 ctcctcgtcg cgtacgacgg ctccgccttc agcggcttcg cccgcaacct cggcgtgacg
      241 accgtcgccg gggagctgga agcgcacctg aggcgggtcc tcaagcacga ggtcgtgatc
      301 accggtgccg gccgtaccga caaaggtgtg cacgcctggg gccaggtcgt caccttcgac
      361 acgtttgccg accgacttca accggagcga cttcagcgtt ccatcaattc ggtgtgcggc
      421 ccggcgatcg cggtgcgcgc catcgacctc gtcgacaccg acttcgacgc ccggttcagc
      481 gccaagtggc ggcggtatcg ctacacgatc ctcacggcag cgacccccaa cccgttcctc
      541 taccggtacg cgtggcatgt caccgacccg atctcggtcg acgcgatgga cgaagccggc
      601 cacgcgatcc tcggagagca cgacttctcg tcattctgtc gccgcccgcc agaccgtcac
      661 gacggcactc cggcgtcgct ggtccgagag gtgaccggcc tgaggtggga gcgactcgag
      721 ggcgacctcc tgcggtgtga gatcaccgcc cgggccttct gtcaccagat ggtgcgcagc
      781 attgtcggca ccctcgtcga cgtcggtcgg ggtcgcctcg ccacggacca ggtcgcaacc
      841 attctcgcgg cacgcgatcg caacgccgcc ggcgacctcg cgccgcccca agggctgtgc
      901 ctccacgagg tcgggtactg atcggtgccg accgtcgggg acggccattt gcggcgccga
      961 ggaccgagcc gatatcgtgt cgagtcgcgt ctgcggccgg tcggctgcgc acgccgacac
     1021 gaatcacccg atcggacacg cggcagccgc caccgtctcg acactcggct cgagatcgac
     1081 cggaccgggc acaccgcaac agaaggttca ccgtgaccac gtactctccc aaagccagcg
     1141 agatcacccg cgaatggcat gtcgtcgacg ccgaaggcat ggtcctcggt cgcctcgcca
     1201 ccgaggtcgc tcgcatcctg cgaggcaagc acaagccgat cttctcgccg cacatggaca
     1261 ccggtgatca cgtgatcatc gtcaacgccg acaaggtcgt cctgaccaag aacaaggccg
     1321 ccgacaagat ggtgtacagc cactccgggt acccgggcgg catcaagtcc tccacctacg
     1381 cccaggacct cgatcgcaag ccggctgacg cagtgcgtcg cacggttcgc ggcatgctcc
     1441 cgaagaaccg gctcggccgc cagcagctca ccaagctcaa ggtgtacgcc ggcccgaccc
     1501 atccgcacca ggcccagcac ccccagacgc tcgtcgtcga gggcgcccgc gcccggtcgt
     1561 agtcctcagc ccgcacactc gtcacgtcgc cgttccggaa agatcgccat gcctcatcct
     1621 ctcgtccagt ccaccggtcg tcgcaagcgg gcggtcgccc gtgtccgctt ccgtccgggc
     1681 accggcacca tcacgatcaa caagcgcccg ctcgaggatt acttcccgaa cgacacgcac
     1741 cgcatggtgc tctccgagcc gctcaaggtc acgtcgacga ccgagtcgta cgacatcgac
     1801 gcgacgatcc atggtggcgg cgtctccggt caggccggcg cgatgcgcct cggcatctcc
     1861 cgggcgctga tcgcactcga cgaggagctt cgcccgaccc tcaagaaggc cggattcctg
     1921 acccgcgact cccgcgagaa ggaatcgaag aagtacggcc tcaagaaggc ccgcaaggcg
     1981 cctcagtact ccaagcgcta gtccggaggg ctcggtgcgg ttcggtaccg acggcatccg
     2041 aggcgttgca aacgcacagc tgacgccaga gcttgctctg gcgatcggcc gcgccgcggc
     2101 acgagtgctc ggcggtcccg tcatcaccgt cggccgtgac cctcgatggt ccggtccgat
     2161 gctcgaggca gcgttcgtcg ccggggcctg tgccgagggc gtgtcggtcg aactgctggg
     2221 cgtcgttccc accccggcag tggcgctgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn gtgctcgact gcgggaacgg cgcctcgtcc gacgttgcgt cgacggtgtt
     2401 cacccgcctc ggggccgagg tcgtcgcgct ccatgtgacc cccgacggca gcaacatcaa
     2461 cgacgccagt ggatccactc accccgagtc attgcgcacc gcagtcgtcg ccgagggggc
     2521 acagctcggg ttcgccttcg acggggacgc cgaccggatg ttggcggtcg atcatcaggg
     2581 tcgtctcatc gatggcgatc agctcatcgc ggtggcggcg gtcgacctca agcgacgcgg
     2641 actgctcgtc aacgacacgg tggtcgtcac cgtcatgagc aacctcgggt tccggatcgg
     2701 catggccgcc gagggcatcg aggtggtgga tacggcggtc ggcgaccggt acgtgctcga
     2761 agccctcgat caggggggcc acgtcctcgg cggcgaacag tcgggccaca tcatcttccg
     2821 tgaccgggcc acgaccggcg acggtgtgct gtcggccgtg gtgatcgcgg agatcgttgc
     2881 gcgtaccggc cgcccgctcg ccgacctggc ggacgctgcg atgacccgac tcccgcaggt
     2941 cctgcgcaac gtccggatcg aggcgccgat gcccgacgta accgtccgga tcgccgacga
     3001 gctgaccgcg gcggccgctg ccctcggcgc cgacgggcgg gtactcgttc gcccgagcgg
     3061 gaccgaaccc gtcgtccggg tgatggtcga ggcggtcgac gcggtgacgg cacaagagtg
     3121 gtgcgaccac ctcgtgcagg tggtcgaatc cctctgatcc ggccgtcggt ccggcgcgga
     3181 tgggctgccg atggaggatc gcccgcgctc ccgtacactc gatgaggcat ccgccagctc
     3241 cgggtgggtg ccggcaggtc aggagagtgt ggaatgtgcg ggatcatcgc ggttgtcaga
     3301 cggccctcgg atcggcctga gccgaccgcc gacgaggtcc tcacgccgct gcgcgaggcg
     3361 ctcgcgctgg tgatcgatcg ggtcgccggt ggagcgccct ccatcgaaca gctcgagcag
     3421 acgacccagc tggtcgcagc ggtcaacctg ctgctccgac cggccaccgg aatggcagcg
     3481 ctgctcggca atccggagct gtcgttcgag gtcgagacgg tcatcgacgc cctcaacgcc
     3541 gaaattctcg agatcgacgg cgtgctcgat gcagggttcg ccggcgcgga cctcgagttg
     3601 gaacaactca acgcggcgat gatcgatctc aaggacgccg tgtggagcgt ggcacgtgac
     3661 cgtctgcggg cggcccgcga ggtggcggcg ctgtccaacg gcgatgtgtc cccggccggc
     3721 gcgatcgcgc tgttcagcct gcaccaggcg ctgtcggcca tcgaccggct cgaggtccga
     3781 ggccgcgact ccgccgggat ccaactcgtg gtgcaccgcc acgacgtcga tctcggggat
     3841 ccggccgtgg ctgccgagat cgcgcgtcgc aacgacgtga acttctcctc gggggcgatc
     3901 cgtgtcgagg gcgactccgt ggtcttcgtg tacaaggcgg cgttcgagat cggtgagttg
     3961 ggcgacaaca ccgccgcgtt gcgggccgca gttcgctccg atgccctcct ccaacggttg
     4021 ctgggtgcac catcggtcga ggcgctcgtg ctcgggcaca cgcggtgggc gtcggtcggc
     4081 atcatcagcg agcccaacgc ccatccgcag agctccgacg agctcgatct cgcagacggg
     4141 cccttgtgga cggctgtcct caacggcgac gtcgacaacc atgccgacat catcgccgcg
     4201 gagtcgatga cgatcgctcc ggccgtcacg accgacgcga aggtgatccc agccctcgtg
     4261 tctcgtcgcc gtgccggcgg tctcgcggcc cacgacgcgt tccgcgactc cgtcgcgtcg
     4321 atggacggat cggtcgccat cggcgccagc caggccggcg accccgagtc gctcttcctc
     4381 gcgctgcgcg gcagcggcca ggccctctac gtcgggctcg ccgacgacgc gttcatcgtc
     4441 gcctccgagc cgtacggcgt cgtcgaggag accagccggt tcctgcggat ggacggcgag
     4501 acgccggcca accccgagaa ccccacggcc agccgcggcc agatcgtcca gctgacggcg
     4561 acgggcgccg gcaccgtcga cggcattcgt cggtggagct acgacggcac cgagctcgtg
     4621 gtgaccgagg acgagctcgc gcacgccgag atcacaaccc gtgacatcga ccgagggtcg
     4681 ttcccccact tcctgctcaa ggagatcacc gacgctccgg tgtcgttccg caagacgctt
     4741 cgcggcaagc tcctcgaacg cgacggcgtc ctctcggtgg agctcggcga gctgaccctg
     4801 ccgacgtcgg tgcgggagcg gatcgccgac ggttcgatcg ggcgggtgat ggtcatcggc
     4861 caggggaccg ccgctgccgc cggagccagc ctggcgctcg cgctcggaga cctggtcgcc
     4921 gacacgacgg tgcgggtcga agcagtgctc gcgaccgaac tctcgggctt ccgacttcgc
     4981 gccgacatga gtgacacgct gatcgttgcg gtgtcccagt cgggaacgac caccgacacc
     5041 aaccgaaccg tcgacctggt ccgcgggcgc ggcgccacgg tcatcgcgat cgtgaaccgg
     5101 cgcaacagcg acctctgtga caagagcgac ggggtgctct acacgtccga cggacgagac
     5161 gtggagatga gcgtcgcctc caccaaggcg ttctacgccc agatcgctgc cggtctcgtg
     5221 ctggccaccg cgatcgccga tctggtgccg ggcgcggtgc acagctcctc gtcgggccag
     5281 cagcaattgc ttgccgcctt gcgcgaactg cctgaccgga tggccgagac ccttgcacgc
     5341 cgagaccaga tcgcgacggc cgcccaccag ttcgggccgc cacgtcggta ctgggcgatc
     5401 gtcggcaacg gccccaacca gatcgccgcg cgcgagatcc ggatcaagct gtccgagctc
     5461 tgctacaagt cgatcgcctg cgacgcgacc gaggacaaga agcacatcga cctctccgcc
     5521 gaaccgatga tcctggtgtg tgccgccggc ctgatcggca gcactgccgc tgacgtcggc
     5581 aaggaagtcg cgatctaccg ggcccacaag gccgcgccga tcgtgatcgc caccgatggt
     5641 gagaacccgt tcgacgctgc gctgtcggtc atctccgtac cggccaccca cgagcgactg
     5701 gccttcatcc tctctgccat ggccgggcac ctcttcggct acgaggcagc cctggcgatc
     5761 gacgcgcagg cccgcccgtt gcgcgaagcg cgctccgtca tcgagagtct cgtcaccgac
     5821 cacgccgacg agctgcggac cggcgagggc gccgtcgagc tgctcgccgg gacggcgcgg
     5881 tcgctgcagc ctctcgcggc gcagttcatg gacggtctcc gctcgggggt ctacaacggc
     5941 catctcgaag ccgcgacggc cgtgaaggtc tcgtcgctgt tccgctacgc aaccggcacg
     6001 agttcgctcg acagctacca gagcgagttc ggaaaggtcg gcacacccgg cgtcgtgctc
     6061 gacgacctca cgaccggcct cacggcggcg atcgaggaac tgacccgtcc ggtcgacgcc
     6121 atcaagcacc aggccaagac cgtcacggtc ggcatcagcc gctccgacga gtccctcctg
     6181 caggtcccgc tggtgctcgg cgtgttggag gccggcgctc ctcgcgaccg gctgtcctat
     6241 gcgacgctcc gctcgctcgc cgagctcgat cccgccgtcg aggacgtgat cgggtacacc
     6301 cgataccggg tcgagaacgg cgaaggtgac gacgcgaccg ccgtcgtcgt ggaccggggt
     6361 ggcatctcga cgggactcag ctcccgtacc gaccgcgatc cccgcctccg cggcaccaag
     6421 cacctcgtgg ctcgcgaaca cgaactcatg gtcgcgaagg gtcgcggcga cggccggacc
     6481 gtgctgatcg tgcccgagac gaaggactcc cagacgacgg ggctcacgct cctccacctc
     6541 cggctccgag accggctccc cgccgccacc gcgcggggag tgctgtcggg gtaccgtcgc
     6601 cggttccagg cattgcgtga cgccgtgacc gagaccgaag atgtgttccg cgatgacctg
     6661 ctcgccgaac aggacaccct cgatctcctg tgcgagccga tcctcgatct cgccgaccgc
     6721 tggcggtcgg cttgatggcc tcggccacct tcgttcggtt cggtcgctgc gctcccttcg
     6781 ttcactccgg tacctcggcg ggttgacggg cccgcgccaa ggagcggacc gggagggacc
     6841 ccggcccgct cactcggcgt gatgggtcac agccgcgggg gcgcgctgct accgccgact
     6901 ccgggggtgg accggagccg ggcgaccctg gagggtggtg gagccgccat cacgaagggg
     6961 tgggcactcg aggaccatgg gcgcggcgat cacggtcgca gctcgacgtc ggcggcgagg
     7021 cgccagcccc ggcgtcggag gttgatgatg gtctcggcac cgagaatctg acggagggac
     7081 atgatcaggc tttcgcagcg tcgggcggac agctcgctga gcccggcgtc gcggatgagt
     7141 tcggcgcgac tgacgaccga gcccttcccg agcaccttca gcacggccat ctcgcggtcg
     7201 gagagctccg acacgacccc gtcgtggatc acgctgctcg c
//
LOCUS       DAOSOA010000145         7239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10337_length_7239_cov_5.814170, whole genome shotgun sequence.
ACCESSION   DAOSOA010000145 DAOSOA010000000
VERSION     DAOSOA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10337_length_7239_cov_5.814170"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1194
                     /locus_tag="PKY13_09945"
     CDS             88..1194
                     /locus_tag="PKY13_09945"
                     /inference="COORDINATES: protein motif:HMM:NF039919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5925 domain-containing protein"
                     /protein_id="HOT80298.1"
                     /translation="MATTSASARRHDRPGTGGPRRAPMQMAAHVDDYWDSIEALAMER
                     FLDGQRFGAVLEFHGVDNLATIAPDANPAAAVVTEYYETWLSLGDTWTFWATRRRDGG
                     MTLRISAVDAATRDKLVASTRAKVPPMPADDGKVAVGFWYFADRPRTSTRRVDAPAWT
                     DIAANYPGAVRPRLVEAMSMVRPSGRGRLLLWHGPPGTGKTTAARALARSWEPWCRTV
                     IVTEPEQLFASTGRILDLLDPDDDDETGCSRWCLLVVEDADELLSAEARSASGQALSR
                     LLNLADGFLGQGMELLILISTNERLAGLHPAVSRPGRALSVLEFPRFDRAEAAEWLGT
                     SLPAAVADASLAELVAHRDGHEAPPAPAPVGQYL"
     gene            complement(1270..3396)
                     /locus_tag="PKY13_09950"
     CDS             complement(1270..3396)
                     /locus_tag="PKY13_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT80299.1"
                     /translation="MATAVVTVVGARGTADAATARTLAEFPYIPHAATEYDRCGELDA
                     AVVGGQVVAPPYSAGLFANARTVASTSTLRRVAGAPVGTLPAEMVIPAGTSAALINQW
                     IALAQTQDAAHTFFVEPGVTSLTAAIVVPPGRPGRKLTLAGLITAPAAAVADSTHAVF
                     EGVEVAGAGRAASYSAVRGDSADVEIRHLEIRLFGDSSKMRTIASPNPSAAFMASAWV
                     VDAANDVNGNGLNSLAAVNTDGAVRWNVIRNWVRLNAGAGVRVGDFNNLWDSCLDQNG
                     QYGVQSYFANDANVWTNEIRDNGLHFKYRLDLRLPMQDDGMSGGTKFWASRNIRTQAN
                     LVRNNHGPGIWYDTNNFDSFIFDNWIEANTREGIMYETSYNAVISDNYLKNNSTTRLP
                     ADDIRTDSSVYISNSSGAQLYENVGGAAVPIRSAMSGRIVVDNNFIEGNKWPITIFHR
                     AGRFCSSELESSGGSANGFSSGACTLTGARSSMSPVPTAVSNRVMSPLAQPRYGTGVN
                     LQYGTDASWWDCSDQSLWWSNRETTFERCAWNTNNVTVVNNKIALPATGPSPSYAAQN
                     GGHIIQIASRATCGSTAPLQYCSFPLVGSKVVNGQPVPVTVKLTGKVRDASRAFEPWV
                     PDDYFLRSMTTGPKANVIRDNQFFVTAGAAGATTYTELGGEPGAGNCTNSTLADPATE
                     VVNFGVWSGAAAAGDNRYCRLLITLQ"
     gene            complement(3500..4510)
                     /locus_tag="PKY13_09955"
     CDS             complement(3500..4510)
                     /locus_tag="PKY13_09955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOT80300.1"
                     /translation="MTVPTDDLSSVRTLYREIEDVGYDRAFSFEAKHDPFLPLATAAE
                     HTDHIQLGTAIAIAFARTPMTLANIGWDLQTLTGGRFTLGLGSQIAPHITQRFSMPWS
                     DPVGRMRELVLGIRAIWDAWTDGTRLSFDGDFYTHTRMIPAFDPGPNEFGRPPILTAG
                     FGPAMTAVSGEVADGFLVHPLNSRRSLLELTVPAIERGLAAAGRPALTDPASTFDLTA
                     VTIVVTGRTAEELDRSREAVRQQLAFYGTTPAYRPVFDVHDRGELHPELKRRGAAGEW
                     VAMAELIDDEFLEEIAVIGEPHEIAPKIRARLTGITDRVSLVNNRAPDPGHFAEVVAG
                     LA"
     gene            complement(4527..5210)
                     /locus_tag="PKY13_09960"
     CDS             complement(4527..5210)
                     /locus_tag="PKY13_09960"
                     /inference="COORDINATES: protein motif:HMM:NF039921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT80301.1"
                     /translation="MADDTSSTTAVPDDELRAADGRVPGRRGRATRDRLTTCTLNMLR
                     DTNYRDLKVVDIAREAGTSPATFYQYFPDVESAILALAEDMAEAGNDRLTRIVRESDW
                     KGKRGYDAAEAIADAYIQFWDDNAALMRVLDLTSLEGDERFRSIRVRLLNEFTIALAG
                     VVEAQRAAGKHPDDLDPMAVAGVLVSMLAHVASHRAGFEGYDIGADDLRRSMARIVFT
                     SVCGTKPPR"
     gene            complement(5263..6888)
                     /locus_tag="PKY13_09965"
     CDS             complement(5263..6888)
                     /locus_tag="PKY13_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HOT80302.1"
                     /translation="MSATAIEGLDSENLTVPGLLARAVERFGDNEAIVDLSVEPPVRL
                     TFAQVADEARATAKALMATGVGPGDRVAIWAPNVWEWPVTLFGIHLAGAAVVPLNTRY
                     KGSEAAYILNRSGAKVLFTVQGFLGNDYVSMLDGLDTPTLEHTIVLRGDTPGGATGCG
                     DFIAAGAGISDDELDARIAGISPDDLADILFTSGTTGAPKGVMCAHRQTVLAFDAWGT
                     IVGLRADDRYLVINPFFHSFGYKAGIIASLVKGNVIVPQATFDVGAAMSLIESEQITT
                     LPGPPTIFQTIINHPDFDIAKLSSLRLAVTGAASIPVQLIKDMRDVLEFDTVLSAYGL
                     TEACGIATVSRHDDPPEIIANYSGRAIPDVEVRIADDEGNEVPRGEQGEVLIRGYQVM
                     IGYFEAPEQTADTIDADGWLHTGDVGIMNEDGYLKITDRTKDMFIVGGFNAYPAEIES
                     LMAEHPDIAQVSVVGVPDERMGEVGYAFVVLKHGATATAADIKAWARGHMANYKAPAT
                     VEIIDELPVNAGGKVMKFELRDRAVASSSAGTP"
     gene            complement(6965..>7239)
                     /locus_tag="PKY13_09970"
     CDS             complement(6965..>7239)
                     /locus_tag="PKY13_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80303.1"
                     /translation="PFDRHALAFESISDDPDGYSFGFVEESTSWMQRRWRHERTVTPL
                     SATTCRVTDRLTVVPRLAGARHVVRRLVPLVFEHRHRRLVARWGAA"
BASE COUNT         1142 a         2435 c         2497 g         1165 t
ORIGIN      
        1 ccgatgagac atcttggtcc atatgtctca acgtagccgc gaaactcgat cgacgtgggg
       61 cgaaccatcg ggcaccgtgg tcgccccatg gccacgactt ctgcatctgc gcgtcggcac
      121 gaccgaccgg gcaccggcgg gccccgccgc gccccgatgc agatggcagc ccacgtcgat
      181 gactactggg actcgatcga ggcgctggcg atggaacggt ttctcgacgg ccaacgattc
      241 ggtgccgtac tggaattcca cggcgtcgac aacctcgcca ccatcgcgcc ggacgcgaac
      301 ccggccgcgg cggtcgtgac cgagtactac gagacctggc tgtcgctggg cgacacctgg
      361 acgttctggg cgacgcgccg ccgggacggc ggcatgacgt tgcgcatctc tgccgtggac
      421 gccgccaccc gcgacaagct ggtcgcatcc acccgggcca aggtcccccc gatgccggcc
      481 gatgacggca aggtcgccgt cggattctgg tacttcgccg accgtcccag gacgagcacg
      541 cgccgagtgg atgcgccggc gtggacggac atcgccgcca actacccggg cgccgtgcga
      601 ccccgcctcg tcgaggccat gtcgatggtg cgcccgagcg ggcggggtcg cctgctcctg
      661 tggcacggcc ctcctggtac cggcaagacc acggcggcgc gagcgctcgc ccgcagctgg
      721 gagccatggt gtcgaacggt gatcgtcacc gagcccgaac agctcttcgc gtcgaccggt
      781 cgaatcctcg acctgctcga tcccgacgac gacgacgaaa cgggttgctc gcggtggtgt
      841 ctgctggtcg tcgaagacgc cgacgagctc ctgagcgcag aggcacgatc cgcctcaggg
      901 caagcgctgt cgcgcctcct caaccttgcc gatgggttcc tcggccaagg catggagctc
      961 ctgatcctca tcagcacgaa cgagcggctc gcgggcctgc atccggccgt cagccgaccg
     1021 ggccgcgcgc tgtcggtgct ggagttccca cgcttcgacc gagcggaggc cgccgaatgg
     1081 ctcgggacct cgctccccgc agcggtggcg gacgcgtcgc tcgccgagct cgtcgcccac
     1141 cgcgacggcc acgaggcacc gccggcgccg gcgcccgtcg gccagtacct ctgaccgctc
     1201 caccctctcg ttctgtcccg cggtcctttc gctgggcgaa agaacagcgg gactcatgga
     1261 gttcggtggc tactgcagtg tgatcaggag gcggcagtag cggttgtcgc cggcggcggc
     1321 agcgccgctc cagacgccga agttcacgac ctcggtcgcc gggtcggcca acgtcgagtt
     1381 ggtgcagttg cccgcaccgg gctcaccacc gagttcggtg taggtcgtcg cgccggcggc
     1441 acccgcggtg acgaagaact ggttgtcacg gatcacgttg gccttcgggc ccgtggtcat
     1501 ggaccgcagg aagtagtcgt cggggaccca cggctcgaag gcccggctcg cgtcgcgcac
     1561 cttgcccgtg agcttcaccg tgacgggcac tggttgaccg ttcacgacct tcgagccgac
     1621 cagcgggaac gagcagtact ggagcggagc ggtcgacccg cacgtcgccc gcgaggcgat
     1681 ctggatgatg tggccaccgt tctgtgccgc gtacgacggg ctcggcccgg tggcgggcag
     1741 ggcgatcttg ttgttgacca cggtgacgtt gttcgtgttc cacgcgcacc gctcgaacgt
     1801 cgtctcgcgg ttcgaccacc agagcgactg gtcggagcag tcccaccacg acgcatcggt
     1861 gccgtactgc agattcaccc ccgtgccgta cctcggttgg gccaagggag acatcacccg
     1921 gttcgacacg gcggtgggca cggggctcat cgacgaccga gcgcccgtca gcgtgcacgc
     1981 tcctgacgag aagccgttgg ccgacccgcc cgacgactcc agctcgctcg agcagaatcg
     2041 accggcccgg tggaagatcg tgatgggcca cttgttgccc tcgatgaagt tgttgtcgac
     2101 gacgatgcga ccgctcatcg ccgaccggat cggcaccgct gccccgccga cgttctcgta
     2161 gagctgcgca cccgacgagt tggagatgta caccgacgag tcggtgcgga tgtcgtcggc
     2221 gggcaggcga gtcgtcgagt tgttcttgag gtagttgtcc gagatcaccg cgttgtagct
     2281 ggtctcgtac atgatccctt cacgggtgtt cgcctcgatc cagttgtcga agatgaacga
     2341 gtcgaagttg ttcgtgtcgt accagatgcc ggggccgtgg ttgttgcgaa cgaggttcgc
     2401 ctgggtccgg atgttgcgcg acgcccagaa cttcgtgccg ccactcatac cgtcgtcctg
     2461 catggggagg cgcaggtcga gccggtactt gaagtgcaga ccgttgtcgc ggatctcgtt
     2521 ggtccacacg ttggcgtcgt tcgcgaagta gctctgcacg ccgtattggc cgttctggtc
     2581 gaggcaggag tcccacaggt tgttgaagtc accgacacgc accccggcgc cggcgttgag
     2641 ccggacccag ttgcggatca cgttccagcg gacggcgcca tcggtgttga cggcagcgag
     2701 gctgttgagg ccgttgccgt tcacgtcgtt cgccgcatcg acgacccagg cgctcgccat
     2761 gaaggcggcg gacgggttgg gggaagcgat cgtccgcatc ttcgacgagt cgccgaagag
     2821 ccggatctcg agatgacgga tctcgacatc ggccgagtca ccacggacgg cggagtacga
     2881 cgcagcgcga ccggccccgg ccacctcgac gccctcgaac accgcgtggg tcgagtcggc
     2941 aacggccgca gccggagcgg tgatgagtcc ggccagggtc agcttccgcc ccggccgacc
     3001 cggcggaacg acgatcgccg ccgtcagcga cgtgacgccc ggctccacga agaaggtgtg
     3061 cgcggcgtcc tgcgtctggg cgagggcgat ccactgattg atgagcgctg ccgacgtgcc
     3121 ggcggggatc accatctcgg cgggcagcgt gcccacgggg gcaccggcga cgcgtcggag
     3181 cgtcgacgtg ctcgccacgg tgcgagcgtt ggcgaagagt ccggccgagt agggcggagc
     3241 gacgacctgc ccgccgacga cggcggcatc gagctcgccg cagcggtcgt actcggtcgc
     3301 ggcgtgcggg atgtagggga actcggccaa cgtccgtgcc gtcgcggcgt cggcagtgcc
     3361 acgagcgccg acgaccgtca cgaccgcggt cgccatcagg aaggcaccag cgatccgagc
     3421 gcgagtacca cgctccgtga acgtctttga atcactttca ccgcgtttca tcactgcaca
     3481 gtaaaagtca cacgccgccc tacgccagac cggcgaccac ctccgcgaag tgcccggggt
     3541 cgggagcccg attgttcacg aggctcaccc ggtcggtgat gccggtgagc ctggcccgga
     3601 tcttcggagc gatctcgtgc ggctcaccga tcaccgcgat ctcctcgagg aactcgtcat
     3661 cgatgagctc cgccatcgcg acccactccc ccgccgcccc acggcgtttc agctcggggt
     3721 ggagctcccc ccgatcgtgc acgtcgaaga cggggcgata ggcgggcgtc gtgccgtaga
     3781 aggccaactg ctggcggacg gcctcgcggg accgatcgag ttcctcggcg gtgcggccgg
     3841 tcacgacgat cgtgacggcc gtcaggtcga acgtgctcgc gggatcagtg agcgccgggc
     3901 gaccggcggc ggccaggccc cgttcgatcg ccggaacggt caactcgagg agcgaccgcc
     3961 gggagttgag cgggtgcacc aggaagccgt cggcaacctc gcccgacacc gccgtcatcg
     4021 ccggaccgaa cccggcggtg aggatgggcg gtcgaccgaa ctcgttcgga cccgggtcga
     4081 acgcggggat catccgggtg tgggtgtaga agtcgccgtc gaacgagagc ctcgtgccgt
     4141 cggtccaggc gtcccagatc gcacggatgc cgagcaccag ctcgcgcatc cgaccgacgg
     4201 ggtcggacca cggcatcgag aaccgctggg tgatgtgcgg ggcgatctgc gatcccaatc
     4261 ccagggtgaa tcgaccgccg gtgagcgttt ggaggtccca cccgatgttg gccagtgtca
     4321 tcggtgtccg ggcgaacgcg atggcgatgg cggtgccgag ctggatgtga tcggtgtgct
     4381 cggctgccgt tgcgagcgga aggaacggat cgtgtttcgc ctcgaacgag aaggcccggt
     4441 cgtagccgac gtcctcgatc tcgcggtaca gggtccggac cgacgacagg tcgtcggtcg
     4501 ggacggtcat cgagacgtgc acgggctcag cgcggaggtt tcgtgccgca cacgctggtg
     4561 aacacgatcc gggccatcga acgacggagg tcgtcggcgc cgatgtcgta gccctcgaaa
     4621 ccggcacggt gcgacgcgac gtgcgccagc atcgagacga gcacgccggc cacggccatc
     4681 gggtcgagat cgtcggggtg cttgccggca gcgcgttgtg cctcgaccac accggcgagc
     4741 gcgatggtga actcgttgag caaccgcacc cggatcgacc ggaaccgctc gtcgccctcg
     4801 aggctcgtca gatcgagcac acgcatgagg gcggcgttgt cgtcccagaa ctggatgtac
     4861 gcgtcggcga tggcttcggc cgcgtcgtag ccccgcttgc ccttccagtc gctctcgcgg
     4921 acgatgcggg tcaggcggtc gttgccggcc tcggccatgt cctcggcaag ggcgaggatc
     4981 gccgactcga cgtcggggaa gtactggtag aaggtcgccg gcgacgtgcc ggcctcccgg
     5041 gcgatgtcga cgaccttcag gtcgcggtag ttggtgtcgc gcaacatgtt gagggtgcac
     5101 gtggtcaacc ggtcacgggt ggcccgaccg cgacgccccg gcacccgacc gtcggccgct
     5161 cgcagctcgt cgtccggtac cgctgtggtg ctgctcgtgt cgtccgccac gtcgctcatc
     5221 ctcgcatggc cccggcccgg cgatcgtcat cggacccggg catcacggcg ttcccgccga
     5281 cgacgacgcc accgcccggt cgcggagctc gaacttcatg accttgccgc cggcgttcac
     5341 cggcaactcg tcgatgatct cgacggtcgc cggcgccttg tagttggcca tgtgcccgcg
     5401 ggcccaggcc ttgatgtcgg cggccgtcgc cgtcgcgccg tgcttcaaca cgacgaacgc
     5461 gtaccccacc tcgcccatcc gttcgtcggg cacaccgacg accgagacct gcgcgatgtc
     5521 ggggtgctcg gccatgagcg actcgatctc cgcggggtag gcgttgaagc caccgacgat
     5581 gaacatgtcc ttcgtacgat cggtgatctt gaggtagccg tcctcgttca tgatcccgac
     5641 atcgccggtg tgcagccacc cgtcggcgtc gatggtgtcg gcggtctgct cgggcgcctc
     5701 gaagtagccg atcatcacct ggtacccacg gatgagtacc tcgccctgct cgccgcgagg
     5761 gacctcgttg ccctcgtcgt cggcgatgcg aacctcgacg tcggggatcg cccggccgga
     5821 gtagttcgcg atgatctccg gaggatcgtc gtggcgggag accgtcgcaa tgccgcacgc
     5881 ctcggtgagg ccgtacgccg acagcacggt gtcgaactcg aggacgtcgc gcatgtcctt
     5941 gatgagctgg acggggatgc tcgctgctcc ggtcacggcc aggcgcaggc tcgacagctt
     6001 cgcgatgtcg aagtcggggt ggttgatgat cgtctggaag atggtgggcg gaccgggcaa
     6061 cgtcgtgatc tgctccgact cgatcagcga catcgccgct cccacgtcga aggtggcctg
     6121 cgggacgatg acgttgccct tcacgagcga cgcgatgatg ccggccttgt acccgaacga
     6181 gtggaagaac gggttgatca cgagatagcg gtcgtcggcg cggaggccca cgatcgtgcc
     6241 ccacgcgtcg aaggcgagca ctgtctgacg gtgggcgcac atcacgccct tgggggcgcc
     6301 ggtcgtgccc gaggtgaaca ggatgtccgc gaggtcgtcg ggtgagatcc cggcgatgcg
     6361 ggcatcgagc tcgtcgtcgg agatgccggc tccagcggcg atgaagtcgc cgcaccccgt
     6421 cgccccgccg ggagtgtcgc cccggagcac gattgtgtgc tcgagcgtcg gcgtgtcgag
     6481 accgtcgagc atcgagacgt agtcgttgcc caggaacccc tggaccgtga acagcacctt
     6541 cgcgccgctg cggttgagga tgtacgcggc ttcgctgccc ttgtaccggg tgttgagcgg
     6601 aacgaccgca gcgccggcaa ggtggatgcc gaacagcgtg accggccact cccacacgtt
     6661 cggggcccag atcgcgaccc gatcgccggg gcccaccccc gttgccatca acgccttcgc
     6721 cgtggcacgg gcctcgtcgg caacctgggc gaatgtgagc cggaccggcg gttcgacgct
     6781 caggtcgacg atcgcctcgt tgtcgccgaa gcgctcgacc gcccgggcca ggaggccggg
     6841 cacggtgagg ttctccgagt ccaacccttc gatcgcagtc gcactcacca gcactccctc
     6901 cacacctgac ggccctcaaa tctgacggac cgtcagaact gtaacagcgg tgggcgccga
     6961 gctctcaggc cgcgccccac cgggcaacga ggcgacggtg gcggtgctcg aacaccagtg
     7021 gcacgagcct ccgcaccacg tggcgggccc cagcgaggcg gggcacgacc gtcagccgat
     7081 cggtcacccg acacgtcgtg gcggacaacg gcgtgaccgt ccgttcgtgc cgccagcgtc
     7141 gctgcatcca tgacgtcgac tcctccacga atccgaagga gtacccgtcg gggtcgtcgg
     7201 agatcgactc gaacgcgagc gcgtggcgat cgaacggca
//
LOCUS       DAOSOA010000146         7197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10403_length_7197_cov_5.813778, whole genome shotgun sequence.
ACCESSION   DAOSOA010000146 DAOSOA010000000
VERSION     DAOSOA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10403_length_7197_cov_5.813778"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="PKY13_09975"
     CDS             complement(<1..2033)
                     /locus_tag="PKY13_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT80304.1"
                     /translation="MTANPELEAEQAYIDHAYACLERTREQARSVRSEVQTGPGGTFQ
                     ARYERDVMIEQADQRLAQTEIGDQSLCFGRIDQLGADEPGPTSARRLEGDRFYIGRVA
                     VSDATSEPVIVDWRAPVAEPFYRATGREPMGLVRRRHFATSGRVLRGIDDEFFGEARD
                     ELESGAVVGQGALISALEEARSGKLGDIVATIQGEQDEVIRAPLPGVLVVQGGPGTGK
                     TVVALHRAAYLLYTHRFPLEGQGVLVLGPNRLFLSYIEQVLPSLGEAGVQIAVLSDMV
                     PNIRASGVDAPDVARIKGELRICDLIAKAVRDRQRRLRETAIIPLGVERLRVTPEDSA
                     ELIASARKRARTHNGGRKFFVEGFYRLVASKMREPLDWHVVRDRIRQLPETREALERM
                     WPQLTPAELLNDLFGSPALTRLAGRKWFTDDELALLRRERADVASDIVWSTNDVPLLD
                     EALELLGPRPKHKDEDAIRTYGHIVVDEAQDMSPMELRVIDRRSLNGSMTIVGDIAQA
                     TSASGHTSWESILRHLPMRREPNRAELLIGYRVPAPSMELASRILTLAAPGLRPPRSI
                     REAGDEPVIERTTGDRFGVDLCAAVGREVAAIGSGSVAVICPSSWVSRVEGALDAGGV
                     DYGQAHRGRFDHQVTVAPVTLVKGLELDACIVIDPQVILDEEYRGPQTLYVALT"
     gene            2166..3233
                     /gene="hemE"
                     /locus_tag="PKY13_09980"
     CDS             2166..3233
                     /gene="hemE"
                     /locus_tag="PKY13_09980"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOT80305.1"
                     /translation="MSEPDVPASPPDLSGSPFLAACRSLPHERIPVWFMRQAGRSLPE
                     YRAIRGEGSILNAIARPDLATEITLQPVRRYGTDAAILYSDIVTPVHAIGFGVDIAPG
                     IGPVVEQPFRSAADLDRLRPLDPEADTPYVIETVKNLVAELPVPLIAFAGAPFTVASY
                     LIEGKPSRTYGLVKTLMLTDEPLWFRLMDRLADLAIASLQSQVGAGASAVQLFDSWAG
                     ALSPSQYERYVQPASRKVFDAVRDLDVPLIHFGVHTGELLGLLADAGADMVGVDWRTP
                     LDVARTRVPDRCALQGNLDPTVVLAGWEPTRAAAADILRRNAGHPGHVFNLGHGVLPE
                     TDPAVLEQLVAWLHEQPVVPT"
     gene            3200..4204
                     /gene="hemH"
                     /locus_tag="PKY13_09985"
     CDS             3200..4204
                     /gene="hemH"
                     /locus_tag="PKY13_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOT80306.1"
                     /translation="MAPRTAGRADVSGPVGVLVMAYGTPSSVSEIEPYYTHIRRGRPP
                     EPHQLADLIARYEAIGGISPLAARTEAQRSAIAAALEARWPGRFLVGLGQKHAAPFIE
                     DGVAALVDAGATSIVGVVLAPHFSGFSVGQYHARGAAAAAEHDVPFDGVDHWYDLPEF
                     VAHQGAALRATLAEMPERTKVVFTAHSLPERVLVGDPYPDQLFASAAAIAGAAGLGRW
                     AGWGVAWQSAGRTPEPWRGPDILQVIDDLAATGRADGIVVVPHGFTSDHLEVLYDVDI
                     EARARAAALGLQLGRPAVVNADETVMAALATRVAATARDDASSAGDAAPGTDPGGAPR
                     "
     gene            4201..5535
                     /locus_tag="PKY13_09990"
     CDS             4201..5535
                     /locus_tag="PKY13_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT80307.1"
                     /translation="MTHIAVVGGGISGLAAAWELSADPRVRVTVHEAADRLGGKIRTS
                     PFAGRAVDEGADAFLRRMPEALALCREIGLEGLIAPAHGTACVWIDGELRPLPGGLVL
                     GVPARFDELAESGILTHWGLARARTEPDLEGEPLVGDTTIGALISRRYGKEVAQRLVG
                     PLLGGIAAGSIDTMSLDAAVPQLAAAAHRSTSLTVALAESLQGAAGSAEPVFAAPPNG
                     MGELIDLLAVRLEERGVDFRLGAPVDGLPVADGIVLTTPADVSARLVAGRSPRAAETL
                     AGIEFASVVFTTLAFRRCDIPGDLDATGFLVPRDAGLTVTAASWSSTKWDRLAGDPVI
                     LRVSMGHSEDHEPIEFDDEEVLARIAGDLGTTMGIRAEPVEHRISRYRDGFPQYAVGH
                     LDRIASLETALAADAPDLAVCGMAHRGVGIPACIREARAAAAALRVRLADAG"
     gene            5560..>7197
                     /gene="lnt"
                     /locus_tag="PKY13_09995"
     CDS             5560..>7197
                     /gene="lnt"
                     /locus_tag="PKY13_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOT80308.1"
                     /translation="MLTAVHFTPSLPTGRWARRGLAVLAGLLSCAAMPPWGWWPLAFL
                     GVGLWALLLADRTWKQRFWVGVLVGQGWYLPSTLWMVKFSPVGWPVGVAIWFPLVFGV
                     LSACCPPRHAALALPGAIVLGEWFRWHAPFGGVPLSMLAYTQARGPLLPVARVGGTLA
                     VSGAVAVVGAAVAALAGRPAGEPGSARARLGGAVALVAVVAVAIGGVVAPDGHRVRTI
                     VAATVQGGGEQQTRSTGNESGLVLDRHMNTAATVKTPVDLVLLPENIVNINGEYRGSY
                     EEQRLTNVATSLHATVVAGVVEDRGSREHFWNFAVAISPDGGLEGRYDKVRRVPYGEY
                     VPMRFLLDPLAKDILPPRDDVEGTGPAVLDTSLGPLGVVISWETFFPRRVREALHHDA
                     QIVLNPTNGSSYWLTQVQTQQIASSTLRAVESGRWVLQAAPTGFSAIIDPRGTVEQRS
                     GMREARVLQQQVELRDGRTLAMIWGEAPALGLAMVALAVAWVLQRRSDREQNVTKSID
                     RGMAVRDPGDYVASIQVVEDANAQPHRDDTDRPLRDGG"
BASE COUNT          971 a         2544 c         2579 g         1103 t
ORIGIN      
        1 gtcagcgcga cgtagagcgt ctggggcccg cggtactcct cgtcgaggat gacctgggga
       61 tcgatgacga tgcacgcgtc gagctcgagg cccttgacca gggtcacggg agcgacggtc
      121 acctggtgat cgaagcgacc acgatgggcc tggccgtagt cgacccctcc ggcgtcgagg
      181 gcgccctcga cgcggctcac ccacgacgag gggcagatga cggcgacgct tcccgatccg
      241 atggcggcga cctcgcgccc gacggcggcg cacaggtcga cgccgaagcg atctccggtc
      301 gtccgttcga tgacgggctc gtcgcctgcc tcgcggatcg agcggggcgg acggagcccg
      361 ggtgcggcga gggtcagaat gcgcgaagcg agttccatcg acggtgcggg cacgcggtag
      421 ccgatgagca gctcggcgcg gttgggttca cgccgcatcg gcaggtggcg gaggatcgac
      481 tcccagctcg tgtgtccgct ggccgatgtc gcctgcgcga tgtcgccgac gatcgtcatc
      541 gatccgttca gtgatcgacg gtcgatgacc cgcagctcca tcggcgacat gtcctgggcc
      601 tcgtcgacga cgatgtgacc gtaggtgcgg atcgcgtcct cgtccttgtg cttgggacgc
      661 gggccgagca gctcgagcgc ttcgtcgagc aggggcacgt cgttggtgga ccagacgatg
      721 tccgatgcga cgtccgcccg ttcgcggcgc agcaacgcca gttcgtcgtc ggtgaaccac
      781 ttgcggccgg cgagtcgggt gagcgccgga gaaccgaaca gatcgttgag cagctcggca
      841 ggcgtgagct ggggccacat gcgctccaac gcctcgcggg tctcggggag ctggcggata
      901 cggtcacgca ccacgtgcca atcgagcggt tcgcgcatct tcgacgcgac caaccggtag
      961 aagccctcga cgaagaactt gcgaccgccg ttgtgggtgc gggcccgctt gcgcgccgac
     1021 gcgatgagct cggcgctgtc ctcgggggtg acgcgaagtc gttcgacgcc gagcgggatg
     1081 atcgccgtct cgcggagcct ccgctggcgg tcacgcactg ccttggcgat gagatcgcag
     1141 atgcgcagct cgcccttgat ccgggcgacg tcgggtgcgt cgacgcccga cgcccggatg
     1201 ttggggacca tgtcggacaa cacggcgatc tggacgccgg cctcgcccag cgacggcagc
     1261 acctgctcga tgtaggagag gaacagccgg ttgggcccga gcaccagcac gccttgtccc
     1321 tcgaggggga agcggtgggt gtagaggagg taggcggcgc ggtggagtgc gacgaccgtc
     1381 ttgccggtgc cgggaccgcc ctgcacgacg agcacccccg gcagtggcgc tcggatgacc
     1441 tcgtcctgct cgccttggat cgtcgcgacg atgtcgccga gctttcccga gcgggcttcc
     1501 tcgagtgcgg agatgagggc accctggccg acgacggcgc ccgactcgag ctcgtcgcgt
     1561 gcctccccga agaactcgtc gtcgatgccg cgcagcaccc gaccggacgt ggcgaagtgg
     1621 cgccgccgga ccaggcccat cggttcgcgg cccgtggcgc ggtagaacgg ctcggccacg
     1681 ggggcgcgcc agtcgacgat gaccggctcc gacgtcgcgt cggacacggc gacgcgtccg
     1741 atgtagaagc ggtcgccttc caggcgtcgc gccgacgtcg ggccgggttc gtcggctccg
     1801 agctggtcga ttcggccgaa gcagagcgac tggtcgccga tctcggtctg ggcgagccgc
     1861 tggtcggcct gctcgatcat gacgtcgcgt tcgtaccggg cctggaaggt gccgccggga
     1921 ccggtctgca cctcggagcg cacggagcgg gcctgttcgc gagtgcgttc gaggcaggcg
     1981 taggcgtggt cgatgtacgc ctgctctgct tccagttcgg gattcgcggt catggcgaca
     2041 cctcggccgt gctgcgattc cgacgggccc ccgtgccggc cgacacaagg gggttcacga
     2101 ttgtaccggt agcgaccggc gatggcgacg tggctcgccg agggagaggt cggtaggttg
     2161 gccgcgtgtc cgaacccgac gttccagcgt ctcctcccga cctgtccggc tcaccgttcc
     2221 tcgctgcctg ccggagcctg ccccacgagc gcatccccgt gtggttcatg cgacaggccg
     2281 gccgctccct gcccgagtac cgagccatcc gcggcgaggg ctcgatcctg aacgcgattg
     2341 cccgacccga cctggcaacc gagatcacgc tccaaccggt gcgccgctac ggcaccgatg
     2401 ccgcgatcct gtactccgac atcgtgacgc ccgtgcacgc catcgggttc ggggtcgaca
     2461 tcgccccggg catcggtccc gtcgtggagc agccgttccg gtcggcggcc gatctcgatc
     2521 gcctccggcc actcgacccg gaggccgaca cgccctacgt gatcgagacc gtgaagaacc
     2581 tggtcgccga actgccggtg ccgctcatcg cgttcgccgg tgcaccgttc acggtggcca
     2641 gctacctgat cgaggggaag ccgtcgcgga cctacggcct cgtgaagacg ctgatgctca
     2701 ccgacgagcc gctgtggttc cggctgatgg accgactcgc cgatctggcc atcgcctcgc
     2761 tccagagcca ggttggggcc ggcgcgtcgg cagtccagct cttcgactcc tgggcgggtg
     2821 ccctctcccc gtcccagtac gagcggtacg tccagccggc gagccgcaag gtgttcgacg
     2881 ccgtgcgcga cctcgacgtg ccactcatcc acttcggtgt gcacaccggc gagctgttgg
     2941 ggttgttggc cgacgccggg gcggacatgg tcggggtcga ctggcggacc cccctcgatg
     3001 tcgcccggac ccgggtaccg gatcgctgtg ccctgcaggg caacctcgac ccgacggtcg
     3061 tgttggccgg atgggagccg acgagggccg cggctgcaga catcctccgc cgtaacgccg
     3121 ggcatcccgg ccacgtgttc aacctcggcc acggggtgct gcccgaaacc gatcccgccg
     3181 tcctcgagca actggtggcg tggctccacg aacagccggt cgtgccgacg tgagcggccc
     3241 cgtcggcgta ctcgtcatgg cctacggcac gccctcgtcg gtgtcggaga tcgagccgta
     3301 ctacacccac atccgccgag ggcgtccgcc cgagccgcat cagctcgccg acctgatcgc
     3361 ccggtacgag gcgatcggcg gcatctcgcc gctggccgcc cggaccgagg cccagcggtc
     3421 ggcgatcgcc gcggcgctcg aggcccgctg gcccggccgc ttcctcgtgg gactcggcca
     3481 gaagcacgcg gccccgttca tcgaggacgg cgtggccgca ctcgtcgacg ccggagcgac
     3541 gtcgatcgtc ggggtcgtgc tcgcgccgca cttctcgggc ttctcggtcg gtcagtacca
     3601 cgcccgcggc gcggcagcgg cggcggaaca cgacgtgccc ttcgacggcg tcgaccactg
     3661 gtacgacctt cccgagttcg tcgcccatca gggcgcagcg cttcgggcga cgctggcgga
     3721 gatgccggaa cggaccaagg tcgtgttcac ggcacactcg ctccccgagc gggtgctggt
     3781 cggcgatccg tatcccgacc aactgttcgc gtcggccgcg gcgatcgccg gcgccgccgg
     3841 tctgggccgc tgggcgggct ggggcgtggc gtggcagagc gccggacgca cccccgagcc
     3901 ctggcgcgga cccgacatcc tccaggtgat cgacgatctg gccgccaccg gtcgggccga
     3961 cggcatcgtg gtcgtgccgc acggcttcac ctccgaccac ctcgaggtgc tctacgacgt
     4021 cgacatcgag gcccgggccc gcgccgccgc gctcggactg cagctcggcc gcccggcggt
     4081 cgtcaacgcg gacgagaccg tgatggcggc cctcgcgacg cgcgtggcgg ccaccgcgcg
     4141 cgacgacgca tcgtccgcag gtgacgcggc gcccggcacc gatcccggcg gcgccccgcg
     4201 atgacgcaca tcgccgtcgt cggcggtggc atctccggtc tggcggcagc atgggagctg
     4261 tcggccgacc cgcgagtgcg ggtcacggtg cacgaggccg ccgaccggct cggcgggaag
     4321 atccgcacgt ccccttttgc cggacgggcg gtcgacgagg gcgccgacgc gttcctccgg
     4381 cggatgcccg aggcactggc cctgtgtcgc gagatcgggc tcgagggctt gatcgccccg
     4441 gcgcacggca cggcgtgcgt gtggatcgac ggcgagttgc gtccactgcc gggagggctc
     4501 gtgctcgggg tccccgcacg tttcgacgag ctggccgagt cgggaatcct cacgcactgg
     4561 ggactcgccc gggcccgaac cgaacccgac ctcgagggcg agcccctcgt gggcgacacg
     4621 acgatcgggg cgttgatcag tcggcggtac ggcaaggagg tcgcgcagcg actggtcggg
     4681 cccctgctcg gcggtatcgc ggccggctcg atcgacacga tgagcctcga cgcggcggtg
     4741 ccccagctgg cggccgccgc acaccggtcg acgtcgctca ccgtcgcgct ggccgagtcg
     4801 ctgcagggcg ccgccggctc ggccgaaccg gtcttcgccg ccccgccgaa cgggatgggc
     4861 gaactcatcg atctcctggc cgtccgcctc gaagagcgtg gcgtcgattt ccggttgggt
     4921 gctcccgtcg acggactgcc cgtagccgac gggatcgtgc tgaccactcc tgccgacgtg
     4981 tcggcccgcc tcgtcgcggg ccggtcgccc cgggccgccg agacactggc cggcatcgag
     5041 ttcgcatcgg tcgtgttcac gacactcgcc ttccgccggt gcgacatccc cggcgatctc
     5101 gacgcgaccg ggttcctcgt cccccgcgat gccggtctca ccgtcaccgc ggcgtcgtgg
     5161 tcgtcgacca agtgggaccg cctcgccggt gacccggtca tcctcagggt gtcgatgggc
     5221 cactccgagg accacgaacc gatcgagttc gacgacgagg aggtgctcgc ccggatcgcc
     5281 ggcgatctcg gcaccacgat ggggatccgg gcagagcccg tggagcaccg gatcagccgt
     5341 taccgcgacg ggttcccgca gtacgcggtg ggccacctcg accgcatcgc ctcactggaa
     5401 acggccctgg cagccgacgc gcccgacctc gcggtctgcg gcatggcgca ccggggtgtg
     5461 ggaattccgg cgtgcatccg cgaggcgcgc gccgccgccg ccgcactccg ggtacggctg
     5521 gccgacgccg gctgacgatc cgtccccgac cgacgtgcga tgctgaccgc cgtgcacttc
     5581 accccctccc tgccgacggg ccgctgggcc cgccgcgggc tcgcggtcct cgccggcctc
     5641 ctgagctgcg ccgccatgcc cccgtggggg tggtggccgc tggccttcct cggcgtcggc
     5701 ctgtgggcgt tgctgctcgc cgaccgtacg tggaagcagc ggttctgggt cggcgtcctc
     5761 gtcggccagg gctggtacct cccgtcgacg ctgtggatgg tcaagttctc cccggtgggg
     5821 tggccggtcg gcgtggccat ctggttcccg ctcgtcttcg gggtcctgtc ggcgtgctgc
     5881 ccgccgcgcc acgcggcgct cgctctcccc ggcgccatcg tcctcggcga gtggttccgc
     5941 tggcacgcac cgttcggcgg tgtccccctg tcgatgcttg cgtacaccca ggcccgcggc
     6001 cccctcctcc cggtggcgcg ggtgggcgga acgctggccg tgtccggagc ggtggcggtc
     6061 gtcggcgcag cggtggcggc gcttgccggt cgcccggccg gcgagccggg cagtgcacgc
     6121 gctcgactgg gtggcgcggt cgcgctcgtg gcggtcgtgg cggtcgccat cggcggggtg
     6181 gtcgcgcccg acggccaccg tgtccgcacc atcgtcgcgg cgacggtgca gggcggcggt
     6241 gagcagcaga cccgctccac cggcaacgag tccgggctcg tcctcgaccg ccacatgaac
     6301 acggcggcga cggtgaagac acccgtcgac ctggtgctcc tcccggagaa catcgtcaac
     6361 atcaacgggg agtaccgagg gtcctacgag gagcaacgac tcacgaacgt cgcgacctcg
     6421 ctccacgcga ccgtcgtcgc cggggtggtc gaggaccggg gcagccgcga gcacttctgg
     6481 aacttcgcgg tggccatcag ccccgacggt gggctcgagg gccgatacga caaggttcgc
     6541 cgggttccct acggcgaata tgtcccgatg cggttcctgc tcgacccgct ggcgaaggac
     6601 atcctcccgc ctcgcgacga cgtggagggc accggtccgg ccgtgctcga cacgagcctc
     6661 ggtccgctcg gggtcgtgat ctcgtgggag acgttctttc cgcggcgcgt ccgcgaggcg
     6721 ctccaccacg acgcgcagat cgtgctcaac ccgaccaatg ggtcgagcta ctggctcacg
     6781 caggtgcaga cgcagcagat cgcgtcgtcg acgctgcggg ctgtcgagag cggccgctgg
     6841 gtcctgcagg cggcgccgac cgggttcagc gcgatcatcg atccccgggg cacggtcgag
     6901 caacggtccg gcatgcgtga ggcccgggtc ctgcaacagc aggtcgaact ccgtgacggc
     6961 cggaccctgg cgatgatctg gggcgaagca cccgcactcg gcctggcgat ggtcgccctc
     7021 gccgtcgcgt gggtgctcca acggcgctct gatcgcgaac aaaatgtcac gaaaagcatt
     7081 gatcggggta tggcggtccg tgatcctggg gactacgttg ccagtattca ggtcgtcgag
     7141 gacgcgaacg cgcagcctca ccgagacgat actgaccgac cattgaggga tggggga
//
LOCUS       DAOSOA010000147         7190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10414_length_7190_cov_5.561458, whole genome shotgun sequence.
ACCESSION   DAOSOA010000147 DAOSOA010000000
VERSION     DAOSOA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10414_length_7190_cov_5.561458"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKY13_10000"
     CDS             <1..457
                     /locus_tag="PKY13_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80309.1"
                     /translation="GPLWIGIAVTAIRFAKSGVAWPDDLRRHWLLLGGAALLAVVPML
                     ATLDETRVYSLTTAPLLVGAAVLSRRTLSKVDGARLTGLAGSRATAAVATTAVLLAAS
                     PGVFTAGKASLSYDLPGVEFARFLVDGDHPGDLTRWLMSPFDFEIPTAN"
     gene            complement(467..1711)
                     /locus_tag="PKY13_10005"
     CDS             complement(467..1711)
                     /locus_tag="PKY13_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOT80310.1"
                     /translation="MPPRFEFSTSGWGQAWVDALESSARLDPSRLGRGRSYARQGAVG
                     ALMIDPGRVRSRVSGSHGRHYVTEVEVRRIPDAAWDPVVDALASKSAHAAALADGELD
                     PAIVADVRAAGIELLPGPGDLRPHCTCPDWAEPCKHAAAVCYLVAAELDRDPFLLFLL
                     RGLPREALVAMMAERRRGGAGGAGDAVRGVVAATAWAGWTLDGREDAAEADADAALPR
                     ALARHIATPAHPGRPAPWEARIAPGDPVDPRRVDELAEDATDRAWLMLADAAPSGLAA
                     SVWGDLARRAGAAHPRELATLAARTRLATSTLNAWAAAWERGGDLGVQVVADEDSWSI
                     DQSRLAAGRDQLVEMGFPKRSVALNYDSLGMPKGVKIVVGPDGRWYHLQFRSGPRGTP
                     DQVHLAAAPSDDIADVIEPPLA"
     gene            complement(1711..4530)
                     /locus_tag="PKY13_10010"
     CDS             complement(1711..4530)
                     /locus_tag="PKY13_10010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT80311.1"
                     /translation="MLLTDRARASEMDLTGVHATILPATPARRSRLALWAPERNDAFS
                     VAADAEPVELVWPAGNGVRRRTVVARQVEIGLLLDALLATGSQPVTGSVAAWSAITKV
                     VLGLVARGRLQPAISAEGDDQWVLSPLDDKDRAVRAHFVEHLPPAAHCLVVGAGPPVR
                     MLSADAAVVAFYQAVADSLPRTAAAPLAVGQTEWASTDPNDVSRLRNVLVGSDDAERT
                     IVGIRLRLPDVDDLPFAVDVQLRSAVDPTLVADAAQLWAGTVAWYEPSFDTDLLRSIR
                     RGAAIWPPLRRLLDQPEPASLELDDDEAMELFGGAAVDLGAAGIEVLIPSALTRTLRA
                     EAHVEPPPGAEDGPSSFDLASVCHLTWRATLDGRPVSDAELAALAASRRPLVKVNDEW
                     VVVDPHVVAKLARRDRMTAADALSAALGGQAVVDDEVVDVVVDGPIADLADRLRRATG
                     PTELADPPGLAAELRPYQRRGVSWIQDMSVLGFGGVLADDMGLGKTIQVIALLLHRAD
                     LRAQAGRPATADGPTLVVCPASVVSNWQREVERFAPGMAVRSYVGTDRSLGGLRGDEV
                     VVTTYGLARRDLANLSEVEWGLVVADEAQHIKNPNSSTAKALRRVPSRARLALTGTPV
                     ENRLTELWALLDWTTPGLLGSVETFRRRISIPIERDRDEDVTARFGRMIAPFLLRRRK
                     DDPAIAPELPPKTETVHTVLLTPEQAGLYKATADAILEQISTSAGIARRGLVLKLLTA
                     LKQICNHPAHYLAQAGPLPARSGKLEAFDELVGAITEAGDSTLVFTQYVVMGDLLVDR
                     LTELGIEARFLNGSVPLSKRSVMVDDFQAGRFPVFVISLKAGGTGLNLTRATHVVHYD
                     RWWNPAVENQASDRAWRIGQDRPVQVHQLISEGTLEERIATVLDAKQALADAVVGSGE
                     AWLTELGNDELAALVELGQTGTGDA"
     gene            complement(4517..5293)
                     /locus_tag="PKY13_10015"
     CDS             complement(4517..5293)
                     /locus_tag="PKY13_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT80312.1"
                     /translation="MATAPTMDYTLTSHGFRLSAHLATPPLASPGPGLVLCHGFPVRG
                     RESPASGKSFPELADRIATEMGWTVLTINFRGCGGSEGDFSMAGWLDDIHAAVVHLIE
                     LGVSGVWLAGYGSGGALCLSEGARNPDVRGVAAIAAPADFDDWARHPRRLVQHSRQVG
                     VIKTAGFPRNFDAWAAEFRQFTAVAAAAGLAPRPLLVMHGEADDLVPSLDARVVADSH
                     GAAELRIIGGGGHELRHDPRAVAVLLGWLSRQHNATVAVD"
     gene            complement(5347..5574)
                     /locus_tag="PKY13_10020"
     CDS             complement(5347..5574)
                     /locus_tag="PKY13_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80313.1"
                     /translation="MKRLGKFEHNRYVGDKRTQVVYDIDELAPEDEPLIEELLAAETF
                     LCFGPDTLAEANNRGYKLFRKQRAAAAAAAD"
     gene            complement(5571..6038)
                     /locus_tag="PKY13_10025"
     CDS             complement(5571..6038)
                     /locus_tag="PKY13_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80314.1"
                     /translation="MIWIFVVVSALVAFVVAAVAVGSVTAAQAGKSRPAVYDLNDAVD
                     HVADHLPADITAVLSFDDVRQVLLWHLDYLQAKGVASYGTDADVDGSLVVVTDDEPIA
                     FILGRADDAEVEITDEQIVAILTAQEGYYRAIGAYGPEVSGPDDPAPSRTMDA"
     gene            6110..>7190
                     /locus_tag="PKY13_10030"
     CDS             6110..>7190
                     /locus_tag="PKY13_10030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT80315.1"
                     /translation="MKQRSNTPLLLAIVAEAMGYGAIFGLLADLQDKFGFQDVGLGLI
                     AASAFPAALVGQLGLSRHADRGHTRRLLWFGLATAAAGMIWFWLGNSLWEFIVARMLV
                     GLGSGTFIPSARRAILARDPHNPGKAISMAGAADIGGFLIGIPVAKQLEGAFDSPNTP
                     FLVLAVILMIIGPLATLIDEPPIHEHHPGGEELRAVFGLPLARAGIFIGLGFAALIGS
                     FDSIASRFLNDLGGTDNELTIVMIALFVPLVVAMPLAGRLVDRIGPVRAGTTALLVAS
                     PVVFAFGLTRHLGVISVLGATVALAFAVVYTSGQAAVAGATVPVGLSGAGQGAYEATY
                     AVGSMICALMAPLIYLKSNAMPMWTV"
BASE COUNT         1044 a         2563 c         2541 g         1042 t
ORIGIN      
        1 gggaccgctc tggatcggca tcgccgtcac cgcgatccgg ttcgcgaagt cgggcgtagc
       61 gtggcccgat gacctccgcc gccactggtt gctgctcggc ggtgcagcgc tcctcgccgt
      121 cgttccgatg ctcgcgaccc tcgacgaaac tcgtgtgtac tccctcacga ccgctcccct
      181 gctcgtgggc gcggccgtgc tgagccggcg cacgctgtcg aaggtcgacg gcgcacggct
      241 caccggcctc gccgggtcca gggcgacggc ggcggttgca acgaccgccg tgctcctcgc
      301 cgcatcgccg ggggtgttca ccgccgggaa ggcatcgctg tcctacgatc ttcccggtgt
      361 cgagttcgcc cggttcctcg tcgacggcga ccatcccggc gacctgacac ggtggctcat
      421 gagtccgttc gatttcgaga tcccgacggc gaactgaacc ggacggtcag gcgagcgggg
      481 gctcgatcac gtcagcgatg tcgtcggacg gtgccgcggc gaggtgcacc tggtcggggg
      541 tgccgcgagg accactgcgg aactgcaggt ggtaccagcg accgtcgggc ccgaccacga
      601 tcttgacccc cttcggcatg ccgaggctgt cgtagttgag tgcgaccgat cgtttgggga
      661 accccatctc gacgagctgg tcgcgaccgg ccgccaagcg ggactggtcg atcgaccagg
      721 agtcctcgtc ggccacgacc tggacaccga gatcgccgcc tcgttcccac gcagcagccc
      781 acgcgttaag ggtggacgtg gcgagacggg ttctggcggc cagcgtcgcg agttcgcggg
      841 gatgggcggc gccggcgcga cgggccagat ctccccacac actcgcggcg agccccgacg
      901 gagccgcatc ggccaacatc agccacgccc ggtcggtcgc gtcctcggcc agctcgtcga
      961 cgcgtcgagg atcgacgggg tcacccggcg cgatccgcgc ctcccacggc gccggacgcc
     1021 ccgggtgcgc cggcgtcgcg atgtgccgag ccagcgcgcg gggcagggca gcatcggcgt
     1081 ccgcctccgc cgcgtcctcg cggccgtcaa gggtccaccc cgcccaggcc gtcgctgcga
     1141 cgaccccgcg gaccgcgtcc cccgccccgc cggcgccacc gcggcgccgc tccgccatca
     1201 tcgccacgag cgcctcacgc ggcagcccgc gcaggaggaa cagcaggaac gggtcgcggt
     1261 cgagctcggc ggcgacgagg taacagaccg cggctgcgtg cttgcagggc tcggcccagt
     1321 ccgggcaggt gcaatggggc cggagatcgc cgggacccgg cagcagctcg ataccggccg
     1381 cccgcacgtc ggcaacgatg gcggggtcga gctcgccgtc ggcgagcgct gcggcgtggg
     1441 cggacttcga cgccaacgcg tcgaccaccg gatcccacgc cgcatcgggg atccggcgca
     1501 cctcgacctc ggtgacgtag tgccgaccgt gggatcccga tacccgcgag cggactcggc
     1561 ccgggtcgat catgagggcc cccacggcac cctgtcgggc gtacgagcga ccccgaccca
     1621 ggcggctggg gtcgaggcgc gccgagctct cgagcgcgtc cacccaagcc tgcccccacc
     1681 ccgacgtcga gaactcgaac cgcggcggca tcaggcgtct cccgtcccgg tctggcccag
     1741 ttcgacgagc gccgcgagct cgtcgttgcc gagctcggtg agccacgctt cgccggagcc
     1801 gaccacagcg tccgcgagcg cctgcttggc gtcgaggacg gtggcgatgc gttcctccaa
     1861 cgtgccctcg gagatgagtt ggtgcacctg caccggacgg tcctgcccga tgcgccaggc
     1921 acggtccgac gcctggttct ccacggcggg gttccaccag cggtcgtagt gcaccacgtg
     1981 cgtcgcccgg gtgaggttga gccccgttcc gccggccttc agggagatga cgaacaccgg
     2041 gaagcgaccc gcctggaagt cgtcgaccat caccgaccgc ttcgagagcg ggaccgagcc
     2101 gttgaggaac cgcgcttcga tgccgagttc ggtgaggcga tcgacgagca ggtctcccat
     2161 caccacgtac tgggtgaaca ccagcgtgga gtcgcccgct tcggtgatgg ctcccaccag
     2221 ctcgtcgaag gcctcgagct tgcccgatcg agcgggcagc ggaccggcct gcgccaggta
     2281 atgcgctgga tggttgcaga tctgcttcag cgcggtcagg agcttgagca ccaaccctcg
     2341 gcgggcgatg ccggccgagg tcgagatctg ctcgaggatg gcgtcggccg tcgccttgta
     2401 gagccccgcc tgctcgggtg tgagcaagac ggtgtgcacc gtctcggtct tcggcggcag
     2461 ctcgggcgcg atcgccgggt cgtccttgcg gcgtcgcagc aggaacggag cgatcatgcg
     2521 accgaaccgg gcggtgacgt cctcgtcccg gtcgcgttcg atcgggatcg agatgcgccg
     2581 tcggaacgtc tcgacgctgc cgagcagccc cggggtcgtc cagtcgagga gcgcccagag
     2641 ttcggtcagc cggttctcga cgggagtacc ggtgagcgcg agacgcgccc gcgacggaac
     2701 ccggcgcagc gccttggcgg tggacgagtt cgggttcttg atgtgctggg cctcgtccgc
     2761 gacgaccagg ccccactcga cctcggacag gttcgcgagg tcgcgacggg ccaggccgta
     2821 ggtcgtgacc accacttcgt cgccgcgcag tcctccgagg ctgcgatcgg taccgacgta
     2881 ggaacgcacg gccatgcccg gagcgaaccg ttcgacctcc cgctgccagt tcgacacgac
     2941 cgacgccggg cacaccacga gcgtcggacc atcggcggtg gcgggacgac cggcctgcgc
     3001 cctcaggtcg gcgcggtgca acagcagcgc gatcacctgg atcgtcttgc cgagacccat
     3061 gtcgtcggcg agcactccac cgaacccgag gaccgacatg tcctggatcc aggagacacc
     3121 acggcgctgg tacgggcgga gctccgcggc gagccccgga ggatcggcga gctcggtcgg
     3181 gccggtcgcc cggcggaggc gatccgcgag gtcggcgatc ggcccgtcga ccaccacgtc
     3241 gacgacctcg tcgtcgacca cggcctggcc cccgagcgcc gccgacaacg cgtcggcagc
     3301 tgtcatccga tcgcgccggg cgagcttggc gacgacgtgc ggatcgacga cgacccactc
     3361 gtcgttcacc ttgaccagcg gtcgccgact ggcggccaac gccgcgagct cggcgtcgct
     3421 gaccggccga ccgtcgagcg tggcgcgcca cgtcaggtgg cagaccgatg cgagatcgaa
     3481 cgacgacggc ccgtcctcgg cacccggcgg tggctcgacg tgggcctcgg cgcgcagtgt
     3541 tcgggtgagc gctgacggga tgaggacctc gatgccggcg gcgccgagat cgacggcggc
     3601 gccgccgaac aactccatcg cctcgtcgtc gtccaactcg agcgacgccg gctccggctg
     3661 gtcgagcagc cgtcgcaacg ggggccagat cgcggcgccc cgccggatgg accgcaacag
     3721 atcggtgtcg aaactgggct cgtaccacgc gacggtgccg gcccacagct gggcagcgtc
     3781 ggcaaccagg gtcgggtcga ccgccgagcg gagctggaca tcgacggcga acggcagatc
     3841 gtcgacgtcg ggcaggcgca agcggatgcc gacgatcgtg cgctcggcgt cgtcggagcc
     3901 gacgagaacg ttgcgcaggc gactcacgtc gttggggtcg gtcgacgccc attcggtctg
     3961 tccgaccgcg agcggcgcgg ccgcagttcg tggcagggag tcagcgaccg cctggtagaa
     4021 cgccaccacc gcggcatcgg cggacagcat ccggacgggc gggccggcgc cgacgaccag
     4081 gcagtgggcc gccggcggaa ggtgctccac gaagtgggcg cgcaccgccc ggtccttgtc
     4141 gtcgagcggg ctcagcaccc actggtcgtc gccctcggcc gagatcgccg gctgcagccg
     4201 gccgcgggcg acgagcccga gcacgacctt cgtgatcgcc gaccacgccg cgaccgatcc
     4261 ggtgaccggc tgactgcccg tggccagcag cgcgtcgagc aggagcccga tctcgacctg
     4321 gcgggcgacg accgtccggc gtctgacacc gttgcccgcc ggccagacca gctcgaccgg
     4381 ttcggcatcg gccgcgacgc tgaacgcgtc gttgcgctcg ggcgcccaca gcgcgagtcg
     4441 actccggcga gccggggtcg ccggaaggat ggtggcgtgg acgccggtga gatccatctc
     4501 ggaggcgcgt gcccgatcag tcaacagcaa cggtcgcgtt gtgctggcgg ctgagccaac
     4561 ccagcaggac cgccacggcg cgtgggtcgt ggcggagttc gtggcccccg ccaccgatga
     4621 tgcgcagctc ggccgcaccg tgggaatcgg ccaccacccg agcgtcgagg ctggggacga
     4681 ggtcgtcggc ctccccgtgc atgacgagca acgggcgcgg cgccaacccc gcggccgccg
     4741 cgaccgcggt gaactgccgg aactccgccg cccacgcgtc gaagttgcgc gggaagccgg
     4801 ccgtcttgat gacgccgacc tgacgggagt gctggacaag ccgtcgcggg tgacgggccc
     4861 agtcgtcgaa gtcggccggc gcggcgatgg cggcgacgcc gcgaacatcg gggttccggg
     4921 cgccttcgga caggcagagt gcgccgccac tgccgtatcc ggcgagccac acacccgaca
     4981 cgccgagctc gatgaggtgc accaccgcgg catggatgtc gtccaaccat cccgccatcg
     5041 agaagtcgcc ctcggagcca ccacagcccc ggaagttgat ggtcaacacc gtccagccca
     5101 tctcggtcgc gatgcggtcg gccagctcgg gaaacgactt gcccgacgcc ggcgactcac
     5161 gcccgcgaac ggggaacccg tgacacagca cgagccccgg gccggggctc gccagcggcg
     5221 gtgtggccag atgcgccgac aggcggaatc cgtgcgacgt caacgtgtag tccatggtcg
     5281 gggcggtggc catgggactc cggggctccg tcgtgggcgg ggtgggcagc gtggcgatcg
     5341 cggacgtcag tcggccgctg ctgcggccgc ggcgcgctgc ttgcggaaca gcttgtaccc
     5401 gcggttgttg gcctcggcga gggtgtcggg gccgaagcac aggaacgtct cggcggcgag
     5461 cagttcctcg atgagcggct cgtcctcggg agccagctcg tcgatgtcgt acaccacctg
     5521 ggtgcgcttg tccccgacgt agcggttgtg ttcgaacttg cccagacgct tcatgcgtcc
     5581 atcgtacgtg acggcgccgg gtcgtcgggt ccgctgacct ccggaccgta ggccccgatg
     5641 gcgcggtagt acccctcctg cgcggtgagg atggcgacga tctgctcgtc ggtgatctcg
     5701 acctcggcgt cgtcggcccg gccgaggatg aacgcgatcg gttcgtcgtc ggtgaccacc
     5761 acgagcgacc cgtcgacgtc ggcgtcggtg ccgtaggacg ccaccccttt ggcctggagg
     5821 tagtcgaggt gccagaggag cacctggcgg acatcgtcga acgacaacac ggcggtgatg
     5881 tccgcaggca ggtggtcggc cacgtgatcg acggcgtcgt tcaagtcgta gaccgccggg
     5941 cgggatttgc cggcctgcgc tgcggtgacc gaacccacgg ccacggctgc cacgacgaat
     6001 gccaccaggg cgctgacgac gacgaagatc cagatcacgg tccgtacgct aggtcctccc
     6061 cgggtgatgg tgtggtgcgg ggatgacgac gccatagagt caacccactg tgaagcagcg
     6121 atcgaacacc cccctcctcc tggccatcgt ggcggaggcc atggggtacg gcgcgatctt
     6181 cggcctgctc gccgatctcc aggacaagtt cggattccag gacgtcgggt tggggctgat
     6241 cgcagcgtcg gccttcccgg ccgcgctggt cggacagctc gggctgtcgc gccacgccga
     6301 ccgcgggcac acccgccggc tcctgtggtt cggcctcgcc accgcggcgg ccggcatgat
     6361 ctggttctgg ttgggcaact cgctgtggga gttcatcgtc gcccgcatgc tcgtgggcct
     6421 cgggtcgggc accttcatcc cctcggcacg ccgcgccatc ctcgcccgcg atccgcacaa
     6481 ccccggcaag gccatctcca tggccggggc cgccgacatc ggtggcttcc tgatcggcat
     6541 tcccgtggcc aagcaactcg agggtgcatt cgactccccg aacacgccgt tcctcgtgct
     6601 cgcagtgatc ctcatgatca tcgggccgct cgccacgctc atcgacgagc cgccgatcca
     6661 cgagcaccac ccggggggcg aggagctccg cgccgtgttc gggctccccc tcgcccgcgc
     6721 cggcatcttc atcggtctcg ggttcgccgc actcatcggc agcttcgact cgatcgcctc
     6781 gaggttcctc aacgatctcg gtggcaccga caacgagctg acgatcgtga tgatcgccct
     6841 gttcgtcccg ctcgtcgtcg cgatgccgct cgccgggcgc ctggtcgatc gcatcggccc
     6901 cgttcgcgcc ggcacgacgg ccctgctcgt ggcctcgccc gtcgtgttcg cattcgggct
     6961 cacccgccac ctcggggtga tctcggtgct cggagcgacc gtcgccctcg cgttcgccgt
     7021 cgtctacacg tccggccagg ccgctgtcgc cggggcgacc gttcccgtcg ggctcagcgg
     7081 tgcggggcag ggcgcgtacg aggccacgta cgccgtgggc tcgatgatct gcgcgctgat
     7141 ggcgccgctc atctacctca agagcaatgc gatgccgatg tggacggtca
//
LOCUS       DAOSOA010000148         7143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10518_length_7143_cov_7.113854, whole genome shotgun sequence.
ACCESSION   DAOSOA010000148 DAOSOA010000000
VERSION     DAOSOA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10518_length_7143_cov_7.113854"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKY13_10035"
     CDS             <1..275
                     /locus_tag="PKY13_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOT80316.1"
                     /translation="IAHVGQHLVADGSATSIGLGRMALSYPDLPADVLAGNDLDRHLV
                     CRTLSDCTTAPRNGLISGCFPLDDFYKDRPERIELAEIKRRLRSAT"
     gene            complement(296..1489)
                     /locus_tag="PKY13_10040"
     CDS             complement(296..1489)
                     /locus_tag="PKY13_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80317.1"
                     /translation="MNDQELSEVIAGIAKALPPSAIDLDVLRRRVAAEDRSRQRGRRN
                     VAAAIAAAAAVAAVIAGVLVAGYDDSSVRIEEPAVRPTSPTSVPTVPSTATAPSPASV
                     TTVVGVADGAAVLVERLDRAAGTVVVELADRVTGAVIRTVEERPAVAGSQSPFLDMDL
                     APDGTAYLAVLGDNPEWVGQLMIVAPDGTERTSLPFVRSVRLSPDGRSLAVVVVSPDG
                     DGDGLGLQAVRVLDIDSERITTVFSTPLDIGPDGTADGGTAWYRIESWSPDGQAVIVT
                     EECCDFSNLFVVRTDGSSAGALPRVDGDAAALLGFDPAGRYIVERRPFNDAGGADLVV
                     VTLDPESGKVSSPILELPDLPDVTAYELVGDQVPGVRTGRGEDALIDRAPFRSEGMDI
                     LSVHT"
     gene            complement(1486..1971)
                     /locus_tag="PKY13_10045"
     CDS             complement(1486..1971)
                     /locus_tag="PKY13_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80318.1"
                     /translation="MKLAKAGWESAFRDHHADLVRLATFLTGDHHAADEVVATVFVRT
                     HDKLVGVADVRAFLRRAVVNGVRDAHRRRVVRMRPMPGAEGSTDPVIEGALIGDQQRR
                     VWRVVQGLPTRQRECVVLRFYEDLTEASIAEILGISRTAVNTNLDRARRRLAAELEGE
                     R"
     gene            2185..2355
                     /locus_tag="PKY13_10050"
     CDS             2185..2355
                     /locus_tag="PKY13_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOT80319.1"
                     /translation="MEFMVARLHAVRNSERGSAIVEYALLVTLVSVACVGAVQKLGVE
                     VSNVFQTAADAL"
     gene            complement(2367..4565)
                     /locus_tag="PKY13_10055"
     CDS             complement(2367..4565)
                     /locus_tag="PKY13_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80320.1"
                     /translation="MTRRLRTSMVVLLVGALCVTACSSGSDDDDAAADSTTTTTDGSA
                     GDVSSGFADRSDDYLAVAAKVELGDDASRDKVWRAVARLAAVPETTTVDLAPAELATI
                     DERFATFTDTTDFDMIALLNLWYRADRGERLSPETREHMKGLILAFKYWYDEPQPAGI
                     VDERWYWSENHQILFHTIEYLAGQAFPDETFTNDGMTGRDHMAHAQPLIEKWVEQRAR
                     YGFSEWYSNVYYQEDLEATVALAEFSDDEALATRGAIASDLVLYDMASHTFDGAFGAT
                     HGRTYKKDKMTALDEDTWDVSKLVLDDTELPYRSDLGAVFMASATKYRPSAVLGEIVA
                     DQGEAPAKGNVGGVVEMARHSLKLDPLTDVVPNPEGPQGLAFDDPDNLMTWWGMAALT
                     PWQTVVETTNEMTKYNLWQTELFSDFKPFEAIVKAAPPDTVRSLARSLAPQLNIGLLS
                     EANTYTWRSGGAMLSTVQDFRKGQASQQHHVWQATFSPDAQVFTTHPRVATEPGVPWH
                     ENTEDWTGNASLPRSAQVHNVNISIYAPLFASKDVLQGSYQEYTHAYLPQEKFDEVVR
                     SDHWTFARLGDEYLALYSWRDPDWVTYDTEAFDTNGLKKPFELIADGGPNNVWITEIG
                     SKGADGSFEEFRKAITSNEPEIRPLGDPTVFSTAFDVSWNSPSQGPITFGWDAPLTVN
                     GTEQPIADYPRIESPWARVPFDSTNYVIKGGKHTLRLDVSAPSRESDADA"
     gene            4660..5685
                     /locus_tag="PKY13_10060"
     CDS             4660..5685
                     /locus_tag="PKY13_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT80321.1"
                     /translation="MAHLPLRIGVIGIEHLHVFEMVDGLVNAGAATICHSAADGPLTT
                     MYEGWRTDSERRSVYGVLGDDSLDLVVLADVPAERGHLAADALVAGRHVLTAKPAVTS
                     PDELDAIDDAVRSSGRRWWVFFSERLCNRAITEAIRLVHAGAIGELVAVAGAAPHTLA
                     ADSRPGWFFDRNRSGGILVDLAAHQADQLLTAAGPGATEVLAATVANVATPTHPGFQD
                     LGRMSLRHTTADGRVVLSDHHVDWLSPAGLGTWGDVRLTLTGTTGSIEVRSNIDVAGA
                     PGGEHLIIVDADSARRVDCSGIAVDWAERLRADIELGTDSFMDHDHTVAACRLALAAQ
                     AHAEADR"
     gene            5682..6704
                     /locus_tag="PKY13_10065"
     CDS             5682..6704
                     /locus_tag="PKY13_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT80322.1"
                     /translation="MSRLRVGVAGLGIGQAHLLAYVLDPERFDVVAIADPDDRRRSRA
                     EADWGVAGVATFEELLDLDLDVVDLCTPPNLHEPQCIAALESGRHVICEKPLVSTLDA
                     LGRLETAEAASSGRLMPIFQYRFAPGIARMKAAVDAGLCGALRLATSETSWLRGDDYY
                     AAPWRGTFAGEGGGAVFGHGTHAHDLLSFVAGPLVSISGQVATLVNDIETEDTATGFG
                     AVADGGLVSMAVTLGSIREITRLRFSFEHVSAESSVEPYEPGNEPWTFDWASAELAAE
                     ADALWASMPETPSGFSGQLAAFHHAVTTDGDLPVTLADARRALELVTGWYESARTGRP
                     VELSGR"
     gene            6739..>7143
                     /locus_tag="PKY13_10070"
     CDS             6739..>7143
                     /locus_tag="PKY13_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT80323.1"
                     /translation="MPALLTERDGHSLIVTLNRPERQNAINGEMLVRLYDACVEADTD
                     PDIRTLILTGAGGNFCSGADLKEMAGGHQGDAAADEIDVQGRLAADGDLPWKALLRTW
                     RPAVPIILAAEGTAIAGGTELLGATEIRVAGAS"
BASE COUNT         1101 a         2524 c         2369 g         1149 t
ORIGIN      
        1 ggatcgcgca cgtgggccaa cacctcgtcg ccgacgggtc ggcgacgtcg atcggcctgg
       61 gtcgcatggc actctcgtat cccgacctcc ccgccgatgt cctcgccggg aacgacctcg
      121 accgccacct cgtctgccgc acactcagcg actgcaccac agcgccccgc aatggcctga
      181 tctcgggatg cttccccctc gacgacttct acaaggatcg ccccgaacga atcgagctcg
      241 ccgagatcaa acgacgactg cggtccgcga cctgatccgg ctcaccgccc ggccttcagg
      301 tgtgcaccga caggatgtcc atcccctcgg agcggaacgg tgctcgatcg atgagtgcgt
      361 cctcgccacg gccggtgcgc acccccggca cctgatcacc gacgagttcg taggcggtca
      421 catcgggcag atccggcaac tccaagatcg gcgaggacac cttgcccgac tccggatcca
      481 aggtgaccac caccaggtca gcgccaccag cgtcgttgaa cggtcgccgc tcgacgatgt
      541 agcggccagc cggatcgaag ccgaggagcg ccgcggcgtc tccgtcgacg cgcggcaagg
      601 cccctgctga gctgccgtcg gtccgaacaa cgaagaggtt ggagaaatcg caacactcct
      661 cggtcacgat cacggcctgc ccgtcgggcg accacgactc gatccgatac cacgcagtcc
      721 cgccatcagc ggtcccgtcc ggcccgatgt ccaacggcgt tgagaagacc gtcgtgatcc
      781 gttcggagtc gatgtccaag acccgcaccg cttgcaggcc caggccgtcg ccgtcgccat
      841 ccggcgacac gacgacgacg gcgagagaac gcccatcggg cgacaggcgc acgcttcgca
      901 cgaacgggag cgacgttcgc tcggtgccgt caggcgccac gatcatgagc tggccaaccc
      961 actccggatt gtcgcccaac acggcgagat acgccgtacc gtcgggtgcc agatccatgt
     1021 cgaggaacgg gctctgcgag ccggcgacgg cgggtcgctc ctcaacggtg cggatcaccg
     1081 caccggtcac tcgatctgcg agctcgacga cgaccgtccc ggcagcacgg tcgagtcgtt
     1141 cgacgagcac cgccgcgccg tcggcgacac cgacgaccgt tgtgaccgac gccgggctcg
     1201 gcgccgtagc ggtggacgga accgtcggga ccgaggtcgg tgacgttgga cgaacggccg
     1261 gttcctcgat gcggaccgac gaatcgtcgt atcccgcgac gagcacgccg gcgatcaccg
     1321 ctgccaccgc agccgctgcc gcgatcgctg cagcgacgtt tcggcgaccg cgttgcctcg
     1381 accgatcctc ggcggcgaca cgccgacgca gcacgtcgag atcgatcgcc gacggcggca
     1441 gtgccttggc gatcccggcg atgacctcgg acagttcttg gtcgttcatc gctctccctc
     1501 cagctcggcg gccagtcgac gacgggcgcg atcgaggttc gtgttgacgg cggtgcggga
     1561 gatcccgagg atctcggcga tcgaggcctc ggtcagatcc tcgtagaacc gcagcacgac
     1621 gcactcacgc tgacgcgtcg gcaacccttg gacgacccgc cacacccgac gctgctgatc
     1681 gccgatcaac gcgccttcga tcaccgggtc ggtcgacccc tcggcgccgg gcatcggccg
     1741 catgcgtaca acacgacgac ggtgggcgtc ccgcacaccg ttgacgacgg ctcgccggag
     1801 gaacgctcga acgtctgcaa cgccgacgag cttgtcgtgc gtccggacga acaccgtcgc
     1861 cacgacttca tcggcagcgt ggtggtcgcc ggtcaggaac gtcgcgaggc ggacgaggtc
     1921 ggcgtggtgg tcacggaatg ccgactccca cccggccttg gccagtttca cgtccacctc
     1981 ccctacccca acgttcgatc ccatgcggat tcctctcgtc cgatcggcgg ctcgatgcca
     2041 ccggacgcga tgtacgacgc agcagcacca cgaattgacc acacggggga ttgacgggcc
     2101 agatttcgtt cttcacattg ggtccgacac gacgggcctg gaagcccgtc tggcgcaacg
     2161 gaagggcgat cgatgttgat ggcaatggaa ttcatggtgg cgcggctcca cgccgttcgg
     2221 aacagcgagc gtggatcggc gatcgtcgag tacgcgctgc tcgtgacctt ggtctccgtc
     2281 gcctgtgtcg gcgccgtgca gaagctcggc gtcgaggtca gcaacgtgtt ccagacggcg
     2341 gccgacgcgc tgtagcgccg accccgtcac gcgtcggcgt cggattcccg gctcggagcg
     2401 ctcacgtcga gccggagcgt gtgcttaccg cccttgatca cgtagttggt cgaatcgaac
     2461 gggacgcggg cccacggcga ttcgatgcgg gggtagtcgg cgatcggttg ctcggtgccg
     2521 ttcaccgtga gcggagcgtc ccacccgaag gtgatcgggc cctgcgacgg cgagttccaa
     2581 ctcacgtcga acgccgtcga gaacacggtg ggatcgccca ggggacgaat ctcgggctcg
     2641 ttcgacgtga tcgccttgcg gaactcctcg aacgagccgt cggcaccctt cgacccgatc
     2701 tcggtgatcc acacgttgtt cggcccaccg tcggcgatca gctcgaatgg cttcttcaga
     2761 ccgttggtgt cgaacgcctc ggtgtcgtag gtgacccagt ccgggtcccg ccacgagtac
     2821 agggcgaggt actcgtcgcc gaggcgggcg aacgtccagt ggtcggagcg gaccacctcg
     2881 tcgaacttct cctggggaag gtaagcgtgg gtgtactcct ggtagctgcc ctggagcacg
     2941 tccttgctcg cgaagagcgg cgcatagatc gagatgttga cgttgtgtac ctgggcgctg
     3001 cgcggcagtg acgcgttgcc cgtccaatcc tcggtgttct cgtgccacgg cacacccggc
     3061 tccgtggcca cccgcgggtg ggtcgtgaac acctgcgcgt cgggcgagaa cgtcgcctgc
     3121 cacacgtggt gctgctggct cgcctgaccc ttccggaagt cctgcaccgt cgagagcatc
     3181 gccccgccac tgcgccacgt gtacgtgttc gcctccgaca gcaggccgat gttgagctgc
     3241 ggagcgagtg aacgggccag cgaccggacg gtatcgggcg gggcggcctt cacgatcgcc
     3301 tcgaacggct tgaagtcgct gaacagctcc gtctgccaga ggttgtactt cgtcatctcg
     3361 ttggtcgtct cgaccaccgt ctgccacggc gtgagcgcgg ccattcccca ccaggtcatc
     3421 aggttgtcgg gatcgtcgaa cgccagcccc tgcggccctt cggggttcgg cacgacgtcg
     3481 gtcagggggt cgagcttcag ggagtgccgc gccatctcga cgacgccacc cacgttgccc
     3541 ttcgccgggg cctcaccctg gtcggcgacg atctcgccga gcaccgccga gggccggtac
     3601 ttcgtcgcgc tcgccatgaa cacggcgccg aggtcggacc ggtatggcag ctcggtgtcg
     3661 tcgagcacca gcttcgacac gtcccaggtg tcctcgtcga gggcggtcat cttgtccttc
     3721 ttatacgtcc ggccgtgcgt cgctccgaac gctccgtcga acgtgtgcga cgccatgtcg
     3781 tagagcacga ggtcgctggc gatggcgccg cgggtggcga gtgcttcgtc gtcggagaac
     3841 tcggcgagcg cgaccgtcgc ctcgaggtcc tcctggtagt acacgttcga gtaccactcg
     3901 ctgaacccgt agcgggcgcg ctgttcgacc cacttctcga tcagcggctg ggcgtgagcc
     3961 atgtggtcgc ggccggtcat gccgtcgttg gtaaaggtct cgtcggggaa cgcctgcccg
     4021 gcgagatact cgatcgtgtg gaacaggatc tggtggttct ccgaccagta ccagcgctcg
     4081 tcgacgatgc ccgccggctg gggctcgtcg taccagtact tgaaggcgag gatcagcccc
     4141 ttcatgtgct cccgggtctc cggggagagc cgctcgccac gatcggcccg ataccagagg
     4201 ttgagcaagg cgatcatgtc gaagtcggtc gtatcggtga acgtcgcgaa ccgttcgtcg
     4261 atcgtcgcca gttcggcggg tgccaggtcg accgtggtgg tctcggggac ggcggcgagg
     4321 cgggccacgg cccgccacac cttgtcgcgg ctcgcatcgt cgcccagctc aaccttggcc
     4381 gccacggcga gatagtcgtc ggaccggtcg gcgaaccccg agctcacgtc gcccgccgat
     4441 ccgtcggtcg tagtggtcgt cgagtcggca gcagcatcgt cgtcgtcact ccccgagctg
     4501 cacgcggtga cgcacagcgc ccccaccagc agcacgacca tcgaggtcct gagtcgtcgc
     4561 gtcatgggcg acgacgctaa ccggccagga cgttcgggca cggcctcccg gcggggcgtc
     4621 ctcggctcgc cttcgtcggg gttgggtagc ttgacctccg tggcccacct acctctccgc
     4681 atcggtgtga tcggcatcga gcatctccac gtcttcgaga tggtcgacgg gctggtgaac
     4741 gccggtgccg cgacgatctg ccactccgct gccgacggcc cgctgacgac gatgtacgaa
     4801 ggatggcgga ccgactccga gcggaggtcg gtgtacggcg tgctgggcga cgactcgctc
     4861 gacctggtgg tgctcgccga cgttcccgcc gaacgcggac acctcgccgc cgatgccctc
     4921 gtcgccggac gccacgtgct caccgccaag ccggctgtga cgtcgccgga cgagctcgat
     4981 gcgatcgacg acgcagtgcg gtcgtcgggt cgtcgatggt gggtgttctt ctccgagcgc
     5041 ctctgcaacc gggcgatcac cgaggcgatc cgcctcgtcc acgccggtgc catcggtgag
     5101 ctcgtggccg tggccggtgc ggccccgcac accctcgccg ccgacagccg gccggggtgg
     5161 ttcttcgacc gcaaccgctc gggaggcatc ctcgtcgacc tcgccgccca ccaggccgac
     5221 cagctcctca cggccgcagg accgggtgcg accgaggtgt tggcagcgac ggtcgccaac
     5281 gtcgcgacgc cgacgcaccc cggcttccag gacctcggtc ggatgtcgtt gcggcacacg
     5341 acggccgacg gtcgggtcgt gttgagcgat caccacgtgg actggttgtc gccggccggc
     5401 ctcggcacct ggggggacgt ccgcctgacg ctcaccggca ccaccggcag catcgaggtg
     5461 cggtcgaaca tcgacgtcgc cggcgcaccc ggcggggagc acctgatcat cgtcgacgcc
     5521 gactcggccc gccgggtcga ctgctcgggg atcgccgtcg actgggccga gcgcctgcgc
     5581 gccgacatcg aactgggaac ggacagcttc atggaccacg accacaccgt cgccgcctgc
     5641 cgcctcgccc tcgcggcgca agcacacgcc gaggccgacc gatgagccgg ctccgcgtcg
     5701 gggtcgctgg gttgggcatc ggacaggcgc acctgctcgc ctacgtgctc gaccccgagc
     5761 ggttcgacgt ggtcgccatc gccgaccccg atgaccgccg gcggtcgcgg gccgaggccg
     5821 actggggcgt cgccggggtc gccacgttcg aggaactcct cgacctcgac ctcgatgtcg
     5881 tcgacctgtg caccccgccg aacctccacg agccccagtg catcgccgca ctcgagtcgg
     5941 gtcgacacgt catctgcgag aaacccctgg tgagcacgtt ggacgcgctc ggccggctgg
     6001 agacggccga ggcggcgtcg tcggggaggc tgatgccgat cttccagtac cggttcgcgc
     6061 ccggcatcgc ccggatgaag gccgcggtgg acgccggcct gtgcggtgcg ctccggctcg
     6121 cgacctcgga gacctcatgg ttgcgcggcg acgactacta cgccgccccg tggcggggaa
     6181 ccttcgccgg cgagggcggc ggcgccgtgt tcggtcacgg cacgcacgcc cacgacctgc
     6241 tcagcttcgt cgccggcccg ctggtgtcga tctccgggca ggtggccacc ctcgtcaacg
     6301 acatcgagac cgaagacacc gcgaccggat tcggcgcggt cgccgacggc gggctcgtct
     6361 cgatggccgt cacactcggc tcgatccgcg agatcacccg actgcgcttc agcttcgagc
     6421 acgtgagtgc ggaatcgtcg gtcgagccgt acgaaccggg caacgaaccg tggacgttcg
     6481 actgggcatc ggccgaactc gccgccgagg cggacgccct gtgggcgtcg atgccggaga
     6541 cgccgagcgg tttcagcggt caactggcgg cgttccacca cgccgtgacg actgacggcg
     6601 acctaccggt cacgctcgcg gacgcccgcc gggcgttgga gctggtcacc ggctggtacg
     6661 agtcggcacg caccggacgc cccgtcgagc tcagcggcag gtagaacgcg ttctcgtttt
     6721 cctctagcct cgcctgccat gcctgctctt ctgaccgaac gcgatggcca ctcgctcatc
     6781 gtcacgctga accgtccgga acgacagaac gccatcaacg gcgagatgct cgtccgcctc
     6841 tacgacgcct gtgtcgaggc cgacaccgat cccgacattc gcaccctgat cctcaccggt
     6901 gccggcggca acttctgctc gggcgccgat ctcaaggaga tggccggcgg ccatcagggc
     6961 gatgccgccg ccgacgagat cgacgtgcag ggccgcctcg ccgccgatgg cgaccttccg
     7021 tggaaggccc tcctccgcac gtggcgcccc gcggtcccga tcatcctcgc cgccgagggc
     7081 accgccatcg ccggtggtac ggagctcctg ggcgccaccg agatccgcgt cgccggtgcg
     7141 agc
//
LOCUS       DAOSOA010000149         7141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10523_length_7141_cov_5.997460, whole genome shotgun sequence.
ACCESSION   DAOSOA010000149 DAOSOA010000000
VERSION     DAOSOA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10523_length_7141_cov_5.997460"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKY13_10075"
     CDS             complement(<1..272)
                     /locus_tag="PKY13_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80324.1"
                     /translation="MDLRTYPDMAAFITDVHDEIAQWLDDSDIGEYTVRPTLGGISYA
                     NQYSVFVVTDSWMEEIFRSTAERNDANEWIAESWEVTLRVSRSACS"
     gene            complement(426..1139)
                     /locus_tag="PKY13_10080"
     CDS             complement(426..1139)
                     /locus_tag="PKY13_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT80325.1"
                     /translation="MKKAIILVALLVVVGLGGFLVYDNMFSSEADEEFTLDSATTDST
                     AGSGGDDNAGGDNAAADLAKLSGTWTVGPTSEAGYRIVEDTPTGETTVTGRTGTVEGS
                     VTLSDGELTATQVAVDLASVASDQPLRDTAFRDTIMKTSEFPKATFRQTDAVAIPGSP
                     SATEPTSVEVAGTLDLRGADQPAIATIDAVADGDTITVVGKIEVQLADFGIDPPKIPG
                     LVTVRDSGTIEFKLVLTKN"
     gene            1267..2625
                     /locus_tag="PKY13_10085"
     CDS             1267..2625
                     /locus_tag="PKY13_10085"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOT80326.1"
                     /translation="MLTIDRLRELHDLGEIDTVLVVFPDMQGRFMGKRVLSSFFLSEV
                     HNNGGSIEACDYLLAVDVDMKVLDGFRFTSWETGYGDFVCRPDLSTLHLVPWLEKTAL
                     VIGDLVDHDGAPIEVSPRAILQRQLGLAADQGYTVKAGTELEFFLFQESMEQAWKQDF
                     KGLTPSSPYVIDYHILQTSKDEWLIRKIRNDMAAAGIPVENSKGEAGLGQHELNLRYA
                     EALEMADNHTIYKNGAKEIAALAGKAITFMAKHHIDEVGSSCHIHSSLWDTASDRSLM
                     PDESGHGLSDTFRWYLGGLLATAAEFALCWAPFINSYKRYQPGSWAPTAIGWGTDNRT
                     LGFRVVGHGQGMRVESRVPGADCNPYIALAATVAGGLYGIENKIEPGDVFAGNGYESD
                     LPRIPHTLVDAIAAWENSEIAKAAFGDDVHFHLLHMARQEWLEFNNHVTDWELRRNFE
                     RL"
     gene            2629..3390
                     /locus_tag="PKY13_10090"
     CDS             2629..3390
                     /locus_tag="PKY13_10090"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOT80327.1"
                     /translation="MTRPLIAITCQRYPAGKLYKLEPAFAVISDYVDAVWRAGGMPMP
                     VFPVPGTDADGGGDASSNATAVLDQVDGVIAIGGLDVDPARYGQERHETTLPASVEQE
                     DFEVAMIEGALDRGTPLLAICRGTQLLNVALDGTLHQHITGRDGLQAHGVPNGGGGTT
                     NDYLLDEGSLVAEVMGGVTASGRCHHHQAVDEVAPGLVVTGRTADGTVEVLELADAAS
                     WMLAVQWHPEETAASDAQNQALFDALVAEAAARNA"
     gene            complement(3457..4533)
                     /gene="gcvT"
                     /locus_tag="PKY13_10095"
     CDS             complement(3457..4533)
                     /gene="gcvT"
                     /locus_tag="PKY13_10095"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOT80328.1"
                     /translation="MTLKTSPLDAAHRDLGAKMVPFGGWDMPLNYSAGTLAEHRACRT
                     SAVAFDVSHLGTVRVSGPGAHDALQRAFTNDLDKVAPGRCQYTHSLDTDGSVADDIIV
                     WWRSDDVFDVMPNASNTDRIVAALTSLAADDVTVEDVGSTRAVIAVQGPQARSMLAAV
                     MPEAATVGRFRCIDVDFDGTTIVAAGTGYTGEDGLELAVPTDRAADVWRAVLSVGIEP
                     AGLGARDTLRLEAGLPLHGHELGPGITTLNADLEWVVSWTKGDFIGRDALAAQKEAGV
                     TPILRGLSVEGKRPPRADMAVLSGAEPVGTVSSGNFSPVLQHGIAMAFLDPSIEIGAT
                     VQLDARGTLVDATVVEMPFITKAS"
     gene            complement(4603..5094)
                     /locus_tag="PKY13_10100"
     CDS             complement(4603..5094)
                     /locus_tag="PKY13_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT80329.1"
                     /translation="MTENNVAAAETVGFSGRRTAEIVGITYRQLDYWARTDLVRPSLT
                     DASGSGSRRQYSYRDLLELKVIKSLLDAGIKLEQVRTVFTYLKEHLGEDVTTANLVIS
                     GTGSVLVRTGEELIDALRSGQGVLNVLQLGGVKDELDASIVDLHPSPDTPEVTYGGAQ
                     HAG"
     gene            complement(5229..5732)
                     /locus_tag="PKY13_10105"
     CDS             complement(5229..5732)
                     /locus_tag="PKY13_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOT80330.1"
                     /translation="MIEVELIGVSVEVASNTPIVVLQELTGSARSLSIFIGGPEATAI
                     AFALEGVQAPRPLTHDLFKDVLEAVNIEIDHVVVSELKEATFFAELHLTNGSDDWIVS
                     ARPSDALALAARVECPIYVNDAVMNEAGVADVVDDDDEVDSGNPEEVVEEFRQFIDSV
                     NPEDFAS"
     gene            complement(5794..6873)
                     /locus_tag="PKY13_10110"
     CDS             complement(5794..6873)
                     /locus_tag="PKY13_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT80331.1"
                     /translation="METTDDIGHLSIGEVLALLQSEFPDVTISKIRFLEAQGLIDPER
                     TPSGYRKFYEPDIVRLRWILRQQRDHFLPLKVIKAKLEQGGEIDFESAIAVQPNLWSE
                     DGAAPAPTPPMEAPWAPRAPGRTRPAAASPQASPAASAVATADADRPAPESTPPVEAP
                     PAPAASPAAPAAAASPAVGPAAGSVTEPSASETVAGAEVEEHNAAAWLAALQESPRKS
                     APTRPAEPTAVGIGRGVRFGLAEVIEVTGVPAEAIESMIEYGLVTPMVIGGEATYDAS
                     SVAVVRAVAQFLARGVEPRHLRMFKNAAEREAGFYEQMILPLLKQRNPKARDQASATL
                     GELAGAGDDLSRALVRQALRSHLGD"
     gene            complement(6860..>7141)
                     /locus_tag="PKY13_10115"
     CDS             complement(6860..>7141)
                     /locus_tag="PKY13_10115"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT80332.1"
                     /translation="GTNAGSRFALDPEVTTIGRHPESTLFLDDVTVSRRHAEIRLEGT
                     HYLVSDVGSLNGTYLNGDRVDVHELAEGDQLQVGKYRLVFAFGAHDGDD"
BASE COUNT         1138 a         2428 c         2455 g         1120 t
ORIGIN      
        1 aatgagcacg ccgagcgcga cacccgcagc gtaacctccc agctctcggc aatccactcg
       61 ttggcgtcgt tgcgctcggc cgtgctgcga aagatctcct ccatccacga atcggtgacc
      121 acgaacaccg agtactggtt ggcgtaggag attccaccaa gggttggcct gaccgtgtac
      181 tcgccgatgt ctgagtcatc tagccactgc gcgatctcgt catgcacgtc tgtgatgaat
      241 gccgccatgt cggggtacgt gcgcaggtcc atgtccacat actccacgag cgatcgacgt
      301 ggccaggacc ctcgtctcat gcgacatggg accagggcgc cttccgtaat ccatgcagaa
      361 ggtgatggtc gacgacacat ttggcatgga tttcgtccgg tcgtcgtgcg tggggacccg
      421 gccggtcagt tcttcgtgag caccagcttg aattcgatgg tgccggagtc gcgcaccgtg
      481 accaggccgg ggatcttcgg ggggtcgata ccgaagtcgg cgagttgtac ctcgatcttg
      541 ccgacgacgg tgatcgtgtc gccgtcggcc acggcgtcga tggtcgcaat cgcaggctga
      601 tcggcgcccc gcaggtcgag cgtgccggcc acctccaccg acgtgggttc cgtcgcggac
      661 ggtgagcccg gaatcgccac ggcgtcggtc tgacggaagg tcgccttcgg gaactccgag
      721 gtcttcatga tggtgtcgcg gaaggccgtg tcccggagcg gttggtccga ggcaacggac
      781 gccaggtcga cggccacctg ggtcgcggtc aactcgccgt cgctcagcgt gaccgagccc
      841 tcgacggtgc ccgtgcgccc cgtgacggtc gtttcgcccg tgggcgtgtc ctcgacgatc
      901 cggtagcccg cctccgacgt cgggccgacg gtccacgtgc ccgacagctt tgcgaggtcg
      961 gccgcggcgt tgtcgccccc agcgttgtcg tcgccacccg aacccgccgt cgaatccgtc
     1021 gtcgccgagt cgagggtgaa ctcctcgtcc gcctccgagg agaacatgtt gtcgtacacg
     1081 aggaacccgc ccagaccgac gacgacgagc agagctacga ggatgatcgc cttcttcatg
     1141 ccgagaagct agggcctctg cgggccgcac cggaccggac ggccgatcgg gcggccccgg
     1201 gccgctacat tctgcggtca ccggcagcga cgccggcgag gcacgtgcca cgagaaggac
     1261 tccgccatgc tcacgatcga ccggctccgc gaactgcacg acctcggcga gatcgacacg
     1321 gtcctcgtgg tgttccccga catgcagggc cgcttcatgg gcaagcgcgt gctcagctcg
     1381 ttcttcctct cagaggttca caacaacggg ggctcgatcg aggcctgcga ctacctgctg
     1441 gccgtcgacg tcgacatgaa ggtgctcgac ggcttccgct tcacgagctg ggagacgggc
     1501 tacggcgact tcgtgtgtcg ccccgacctg tccacgctcc acctcgtgcc gtggctcgag
     1561 aagacggccc tcgtcatcgg cgacctcgtc gaccacgacg gcgcccccat cgaggtgtcg
     1621 ccccgggcga tcctgcaacg gcagctcggc ctcgccgccg atcagggcta caccgtcaag
     1681 gccggcaccg agctcgagtt cttcttgttc caggagtcca tggagcaggc gtggaagcag
     1741 gacttcaagg gcctcacgcc gtcgagcccg tacgtcatcg actaccacat cctccagacg
     1801 tcgaaggacg agtggctgat ccgcaagatc cgcaacgaca tggcggcggc gggcatcccg
     1861 gtcgagaact ccaagggcga ggccggcctc ggccaacacg agctcaacct gcgctacgcc
     1921 gaggcgctgg agatggccga caaccacacc atctacaaga acggcgccaa ggagatcgcg
     1981 gccctcgcgg gcaaggccat cacgttcatg gcgaagcacc acatcgacga ggtcggcagc
     2041 tcgtgccaca tccactcgtc cttgtgggac acggcgtccg atcgttcgct catgcccgac
     2101 gagtcgggcc acggcctgag cgacaccttc cgctggtacc tcggcgggct cctcgccacc
     2161 gcagcggagt tcgccctgtg ctgggcaccg ttcatcaaca gctacaagcg ctaccagccc
     2221 ggctcgtggg cgcccaccgc catcggctgg ggcaccgaca accgcacgct cggcttccgg
     2281 gtggtgggcc acggccaggg catgcgggtc gagagccggg tgcccggcgc cgactgcaac
     2341 ccctacatcg cgctggccgc caccgtcgcc ggtgggctct acggcatcga gaacaagatc
     2401 gagcccggtg acgtgttcgc tggcaacggc tacgagtccg acctgcctcg catcccccac
     2461 acgctcgtcg acgccatcgc ggcgtgggag aactcggaga tcgccaaggc cgcgttcggt
     2521 gacgacgtcc acttccacct gctgcacatg gcccgccagg aatggctgga gttcaacaac
     2581 cacgtcaccg actgggagct ccgccgcaac ttcgagcggt tgtaggccat gactcgcccc
     2641 cttattgcca tcacctgcca gcgctacccg gccggcaagc tctacaagct cgagccggcg
     2701 ttcgccgtca tcagcgacta cgtcgacgcg gtctggcgag cgggtggcat gccgatgccg
     2761 gtcttcccgg tgccggggac ggacgccgac ggagggggcg acgcgtcgag caacgccacg
     2821 gccgtgctcg accaggtcga cggggtgatc gccatcggcg gtctcgacgt cgaccctgcc
     2881 cggtacggcc aggagcgcca cgagaccacg ttgccggcgt cggtcgaaca ggaggatttc
     2941 gaggtcgcga tgatcgaggg cgcactcgac cgcggcactc ccctgttggc catctgccgg
     3001 ggcacgcagc tcctcaacgt ggcgctcgac ggcaccctcc atcagcacat caccggccgc
     3061 gacgggctcc aggcgcacgg tgtccccaac ggcggcgggg gaacgacgaa cgactatctc
     3121 ctcgacgagg ggtcactggt cgccgaggtc atgggaggcg tgacggcgtc gggtcgctgt
     3181 caccaccatc aagcggtcga cgaggttgct cccggcctcg tcgtgaccgg ccgcaccgct
     3241 gacggcacgg tcgaggtgct ggagctggcc gacgcagcgt cctggatgct ggcggtgcag
     3301 tggcaccccg aggagacggc ggcgagcgac gctcagaacc aggcgctctt cgacgccctg
     3361 gtcgccgagg ccgccgcccg aaacgcgtga acctcggggg tttgtgctgc gaggagcgac
     3421 gtcagcgtcg ctcctcgcac cacaaaaccc cctgggtcag gaggctttgg tgatgaaggg
     3481 catctcgacg acggtggcgt cgacgagggt gccgcgggca tcgagctgga cggtcgcccc
     3541 gatctcgatc gacggatcga ggaacgccat ggcgatgccg tgctgcagca ccggcgagaa
     3601 gttgcccgac gacacggttc cgaccggctc ggcacccgac agcacggcca tgtcggcacg
     3661 cggcgggcgc ttgccctcga cgctgaggcc acggaggatc ggcgtgacac cggcctcttt
     3721 ctgggcggcg agggcgtcgc ggccgatgaa gtcgcccttg gtccacgaca cgacccactc
     3781 gaggtcggcg ttgagcgtgg tgatgccggg gccgagctcg tggccgtgca acggcaggcc
     3841 ggcctcgagc cggagcgtgt cgcgggcgcc gagcccggcc ggctcgatgc ccaccgacag
     3901 cacggcgcgc cacacatcgg cggcccgatc ggtgggaacg gcgagctcga ggccgtcctc
     3961 gccggtgtag ccggtgccgg cggcgacgat cgtggtgccg tcgaagtcga cgtcgatgca
     4021 ccggaaccga cccaccgtcg cggcctcggg catgacggcg gccagcatcg agcgggcctg
     4081 cggcccctgc acggcgatca ccgcccgggt cgagccgacg tcctcgacgg tcacgtcgtc
     4141 ggcagccaac gacgtgagcg ctgccacgat gcggtcggtg ttggaggcgt tcggcatgac
     4201 gtcgaagacg tcgtcggatc gccaccacac gatgatgtcg tcggcgaccg atccgtcggt
     4261 gtcgagcgag tgggtgtact ggcaccgacc cggcgcgacc ttgtcgagat cgttggtgaa
     4321 tgcccgttgc agcgcgtcat gggcgccggg acccgacacc cgcacagtgc cgaggtggct
     4381 gacgtcgaac gccaccgcag aggtgcggca ggcccggtgc tcggcgagcg tgcccgccga
     4441 gtagttgagc ggcatgtccc agccaccgaa cggcaccatc ttggcgccga ggtcgcggtg
     4501 ggcagcgtcg agcggggagg tcttgagcgt cacgggccga ccttactggc cccgacgcgg
     4561 ggcgaacgaa gcctcgcggt gcgccaccgg cgccggaggg cgtcagcctg cgtgctgggc
     4621 gccgccgtag gtgacctcag gggtgtcggg gctcgggtgc agatcgacga tgctcgcgtc
     4681 gagctcgtcc ttcacaccac cgagctgcaa cacgttgagg actccctggc cggaacggag
     4741 tgcgtcgatg agctcctcgc cggtgcggac gagcaccgaa ccggtgccac tgatgacgag
     4801 gttggcggtc gtcacgtcct cgccgaggtg ttccttcagg tacgtgaaga ccgtgcgcac
     4861 ctgctcgagc ttgatgcccg cgtcgaggag gctcttgatg accttgagct ccaggaggtc
     4921 gcggtaggag tactggcgcc ggctcccgct gcccgaggca tcggtgaggg acgggcgcac
     4981 gaggtcggtg cgcgcccagt agtcgagctg ccggtacgtg atccccacga tctcggcggt
     5041 tcgacgccct gagaatccga cggtctcggc tgctgcaacg ttgttctcgg tcattcctgc
     5101 cctcacgaca tcggtgaaac tacgtgcgtg tcaacatacc gagggccgcg cgtcaaggca
     5161 acgaccattc gcgcgatccg cgcgattatt cgcgcggatc gcgcgaacgc cgcctcgggc
     5221 ggcggtgatc aggacgcgaa gtcctccgga ttgaccgagt cgatgaactg gcggaactcc
     5281 tccaccacct cttcggggtt gcccgagtcg acctcgtcgt cgtcatcgac cacgtcggcg
     5341 acgccggcct cgttcatgac tgcgtcgttg acgtagatcg ggcactcgac tcgagcggcg
     5401 agggccaggg catcggacgg gcgagccgac acgatccagt cgtcgctgcc gttggtgagg
     5461 tggagctcgg cgaagaaggt cgcctccttg agctcgctca cgaccacgtg gtcgatctcg
     5521 atgttgaccg cctcgaggac gtccttgaag agatcgtggg tgagcgggcg tggcgcctgc
     5581 accccctcga gggcgaaggc gatcgccgtc gcctcggggc cgccgatgaa gatcgacaac
     5641 gatcgcgcag accccgtcag ctcctgcaac accacgatgg gcgtgttgga ggcgacttcc
     5701 acactgacac cgatcagctc gacctcgatc attcccggaa cgatagcccg ctcccacccc
     5761 cgtcagggag ggcaggcgcg ggtgatgttc gggtcagtcg ccgaggtgcg accggagggc
     5821 ctgacgcacg agcgcccggc tcaggtcgtc acccgccccg gccagctcgc ccagggtcgc
     5881 cgacgcctgg tcgcgggcct tgggattccg ctgcttgagc agcggcagga tcatctgctc
     5941 gtagaacccg gcttcgcgct cggcggcgtt cttgaacatc cgcaggtgcc gtggttcgac
     6001 accacgggcc aggaactgag cgaccgcccg caccacggcc accgacgacg cgtcgtaggt
     6061 ggcttcaccg ccgatgacca tcggtgtcac caggccgtac tcgatcatcg attcgatcgc
     6121 ctcggccggg accccggtca cctcgatcac ctcggccagc ccgaaccgga ccccgcggcc
     6181 gatgccgacg gcggtcggtt cggccggacg cgtcggcgcc gacttgcgcg gggactcctg
     6241 cagcgccgcc agccacgccg cggcattgtg ctcctcgacc tcggcgccgg cgacggtctc
     6301 cgacgcggag ggctcggtga ccgaaccggc cgccggaccg acggcagggc tcgcagcggc
     6361 agcgggcgcg gcagggctgg cagcaggcgc gggcggcgcc tcgacgggcg gggtggactc
     6421 cggtgcggga cgatcggcgt cggccgtcgc gaccgcagac gctgccggcg atgcctgggg
     6481 tgatgcagcg gcgggacggg tgcgaccggg tgcccgtggc gcccacggtg cctccatcgg
     6541 tggagtcggc gccggagcgg caccgtcctc ggaccacagg ttgggctgca cggcgatggc
     6601 tgactcgaag tcgatctcgc caccctgctc gagcttcgcc ttgatgacct tcagtggcag
     6661 gaagtggtca cgctgctggc gcaggatcca acgcagccgg acgatgtcgg gctcgtagaa
     6721 cttccggtag cccgacggcg tgcgctcggg atcgatgagg ccctgcgcct cgaggaaccg
     6781 gatcttcgag atcgtgacgt cggggaactc cgactgcagg agcgccagca cctcgccgat
     6841 cgagaggtgc ccgatgtcgt cagtcgtctc catcgtgcgc tccgaatgcg aagaccaacc
     6901 ggtacttgcc gacctgcagt tggtcaccct cggcgagctc gtgcacgtcg acacggtcgc
     6961 cgttgaggta ggtgccgttg agcgatccga cgtcgctgac gagatagtgg gtgccctcga
     7021 ggcggatctc ggcgtggcgg cgcgacaccg tcacgtcgtc gaggaacagc gtgctctcgg
     7081 ggtggcgacc gatcgtggtc acctcgggat cgagtgcgaa tcgcgagccg gcgttggtgc
     7141 c
//
LOCUS       DAOSOA010000150         7124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10553_length_7124_cov_5.796718, whole genome shotgun sequence.
ACCESSION   DAOSOA010000150 DAOSOA010000000
VERSION     DAOSOA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10553_length_7124_cov_5.796718"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..1238)
                     /locus_tag="PKY13_10120"
     CDS             complement(306..1238)
                     /locus_tag="PKY13_10120"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT80333.1"
                     /translation="MDTTTATTTDPIETDWDVIIVGRSFAGLSAALTLGRARRSVLVV
                     GTGGPRNEAVGHAHGMLSRDHESPTAIIAVAEAQLARYPNIELVDDRVLAIEPIGPGA
                     GFRATVGRRTTTATGVVLANGVNDDPMPIPGVAEHWGRGVFTCPFCDGWEHRDLHLAV
                     IGDPAFAPHTGQLLTGWSDRVTVFCDLDADVVERLAGFGVAVDDRPVARVLGDGAAVS
                     GIELEDGTVVAVGAVFHARLPVPNTPLAVSLGCELAPMGFVVTDEMQRTTVDGVWAAG
                     DLTGMRHQMTFAIADGAAAASSLVAYLMGTLPPS"
     gene            1287..1880
                     /locus_tag="PKY13_10125"
     CDS             1287..1880
                     /locus_tag="PKY13_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOT80334.1"
                     /translation="MTQDLAHPADVDGSLEETARRRLRSLRTARGWSLDELARRSHIG
                     ASTISRIETGHRRVALDQLVSLARALGTSVDDLLATPEEDVVIRPERDTAGGVTTWPL
                     TRHHDASGRVVVKMRFPKVTGRPSPQVHPGRDWFYVIEGTARLFLGDREHLVEAGQAA
                     EFDTMTPHFVGGYGGPVEVISIFDRHGELAHLAHGPR"
     gene            complement(1935..2801)
                     /locus_tag="PKY13_10130"
     CDS             complement(1935..2801)
                     /locus_tag="PKY13_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT80335.1"
                     /translation="MSDTTISAVPSGTFSIGGDLPVVRLGYGAMQITGPGVWGPPRDH
                     AEALAVLRRAVELGVNFIDTADSYGPYVSEELIREALHPYADDLVIATKAGLVRTGPG
                     EWWPVGRPEYLRQEAELSLRRLGLERIDLFQLHRIDPQVPVEEQIGVLVNLQTEGKIR
                     HIGLSEISVDELRTAQAITSIATVQNRYNLIDRASEALVDVCDDEGIGFIPWFPIATG
                     ELAKDGGPLAALADETGATPAQLALAWLLHRSASMLPIPGTSSVAHLDDNVGAAAVSL
                     TADQIATLDGLA"
     gene            2970..3455
                     /locus_tag="PKY13_10135"
     CDS             2970..3455
                     /locus_tag="PKY13_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80336.1"
                     /translation="MGMIRKTMSIGTLGLVSWRSKAEKLEIAEYVLAQAQSRTEAEHA
                     ARLGAEARADLATAALARSEKRTRRQRRREETKTLLAKSSEAVEEIGHAVAHSAKAAE
                     PKVRRARRHARRSAKQARRTATSRAQDLAGTVREQAEAAKETVLDQAEVIRDRAASLT
                     D"
     gene            complement(3508..4953)
                     /locus_tag="PKY13_10140"
     CDS             complement(3508..4953)
                     /locus_tag="PKY13_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT80337.1"
                     /translation="MRRQLTVFVVLGALLASGCGGAKGSDKADTSSSSDGTTATTAGG
                     SDSSAPDGGAGKFGSIESPCGPGDAKVKADQAGAGADKLYIGVANDRTSIRNGLLKEL
                     FDGATGFAKWCNEQGGVAGLPVEVVDMDGKLFEIEAAMAKACTGVFAMVGGGYAQDNL
                     IFTGKDGSDFHKCGMVAVPGFAVSTEFAEASDQIQAIPNPPHLKGVSHFEGLKSVYPD
                     KIAKYGVAFGDLPSIVQNKDQTVATVKAVEGYGDPVEISYDPINQDFAVTAQQVIDKG
                     LELVSYIGEPSGLSKFSQALKDQGWDGVITADANQYDSRLLAASGPAAVEGVTVRSNM
                     HPFEEADEWPATKQFMEIMDANVDGWEHAVLAIQAFSSTLLFATLAKQCSEDGEITRE
                     CIMEKGLEVHDWDGGGLHSSGDPGANTPGSCSMIVHVENGTWTRLYPEVGSDDDDGDG
                     FHCGDVVEVTGNFGEGNQESSVLGTWPVKTS"
     gene            5318..6649
                     /locus_tag="PKY13_10145"
     CDS             5318..6649
                     /locus_tag="PKY13_10145"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HOT80338.1"
                     /translation="MTASTGPDLAAAGYTETEFAASGTATAYTQDGELPADGRLALRP
                     TTSAPYSTRVVVRRPATDAAFNGTMVVEWLNVSSGADASPDYAYLSDELVRKGYAWAG
                     VSAQRIGIEGGDVAVPVSLGIDTGAGKGIRTIDPERYGALRHPGDAYSYDIFTQVGRA
                     LRGDSAPGPATDGLEVDRVLAVGESQSAFALTTYANGVQPLTDAFDGFLIHSRGGAGA
                     PLGAPDSGIDITSTVLGPPTLLRTDLDVPTIVVQTETDVLGILNYYPSRQPDTDRLRV
                     WEVAGTAHADRFQIGPVESALGCAGPINNGQQAFVLRAALRDLDEWAAGGDAPPTAEP
                     LTIEESAGSPEFVLDEVGNVTGGVRTPAVEAPTAVLSGLPADGASIVCILMGSTSPFP
                     ADQLAARYPGPDDYATAWAAATDATIDAGFFLPEDRAAIIADARPTGLAAG"
     gene            complement(6678..>7124)
                     /locus_tag="PKY13_10150"
     CDS             complement(6678..>7124)
                     /locus_tag="PKY13_10150"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80339.1"
                     /translation="GAAEAAVGMYRERLATRVLAYTLGDQAAEQPAAQVRLATATSDV
                     AAARHHWEASIAAVERGGAAGAVTEELRVSARLAAAATVRSARRIVTDVCDGAGASVY
                     FSSHPLQRLQRDVETLKGHVMFDWDRTAELAGRHALGFGLRPTDMV"
BASE COUNT         1072 a         2573 c         2468 g         1011 t
ORIGIN      
        1 ggtcgagaac acggtcgaca aggccaagga caagctgcac aagtagccgt cactgacgac
       61 ggctcggccc ggttcgcccg gccgagccgt cgtcagctcg tgcagttcaa gtcgagcgtc
      121 gccacgggta cgtcccacga gttcgggtcc tccacgggca tccgctcgca ggcaggagcg
      181 gccgtctccc tccgccggtc ggaacggcac cacgacctca ccgtcctcac gcggaccatc
      241 gccctcacgc ggaccatcgt cgcgccgacc cccggggtca gaacggtggt ctcgggacgg
      301 cggtctcagg acggcggcag cgtgcccatc agatacgcca cgagcgagga agcggcggcg
      361 gcgccgtcgg cgatggcgaa cgtcatctga tggcgcatcc cggtgaggtc cccggcagcc
      421 cagacgccgt cgacggtcgt tcgctgcatc tcgtcggtga ccacaaaacc catcggtgcg
      481 agctcacagc cgagcgacac cgccaatggc gtgttcggca cggggagacg agcgtggaag
      541 accgccccga cagcgacgac ggtgccgtcc tcgagctcga tccccgacac ggcggcgccg
      601 tcgcccagca cccgcgccac gggtcggtcg tcgaccgcca cgccgaagcc ggcgagccgt
      661 tcgaccacgt cggcgtcgag gtcgcagaac acggtgaccc ggtccgacca cccggtgagg
      721 agttgcccgg tgtgcggagc gaacgccggg tcgccgatca ccgccaggtg cagatcccgg
      781 tgttcccagc catcgcagaa cggacaggtg aacacgccgc gaccccagtg ttcggcaacg
      841 ccggggatcg gcatcggatc gtcgttgacg ccgttggcga gcaccacgcc cgtcgccgtg
      901 gtcgtgcgac gcccgacggt cgcccggaag cctgccccgg gaccgatcgg ttcgatggcg
      961 aggacccggt cgtcgacgag ctcgatgttc gggtaccgag cgagctgtgc ctcggccacg
     1021 gcgatgatgg cggtgggcga ttcgtgatcg cggctcagca tgccgtgggc gtgaccgacg
     1081 gcctcgttgc gaggcccgcc ggtcccgacc acgagcaccg agcggcgggc acgcccgagg
     1141 gtgagcgctg cgctgagccc ggcgaaactg cggccgacga tgatgacgtc ccagtcggtc
     1201 tcgatcgggt cggtggtggt ggccgtagtg gtgtccatgt cggcgaacgt acccaacccg
     1261 agcccgagtg gcataggctc ttgccaatga cgcaagatct cgcccacccc gccgacgtcg
     1321 acggctcgct cgaggagacc gcgcggcgcc ggctccgctc gttgcgcacg gcacgggggt
     1381 ggtcgctcga tgaactcgca cgccgcagcc acatcggcgc gtccacgatc agtcgcatcg
     1441 agacggggca ccggcgcgtg gcgctcgatc agctcgtcag cctggcccgc gccctgggca
     1501 cgagcgtcga cgacctgctc gccacccccg aggaggatgt cgtcatccgc cccgagcgtg
     1561 acaccgccgg cggtgtcacg acgtggccgc tcacccggca ccacgacgcg tcgggccggg
     1621 tcgtggtcaa gatgcgcttc cccaaggtga ccgggcggcc gtcaccccag gtgcacccgg
     1681 gccgggactg gttctatgtg atcgagggga cggcgcggct gttcctcggc gatcgtgagc
     1741 atctggtcga ggccggccag gcggccgagt tcgacacgat gacaccgcac ttcgtcggcg
     1801 gctacggcgg acccgtcgag gtgatcagca tcttcgaccg gcacggagaa ctggcccacc
     1861 tcgcacacgg cccccgctga cccgaaccgc gcaggcggcc cgtctcaggc gagcccgcct
     1921 caggcgagcc cgtctcaggc gaggccgtcg agcgtggcga tctgatcggc cgtcaacgac
     1981 accgccgcgg caccgacgtt gtcgtcgagg tgggcgaccg acgacgtgcc ggggatcggg
     2041 agcatcgagg cggaccggtg cagcaaccac gcaagggcga gctgcgccgg cgtggccccg
     2101 gtctcatcgg cgagcgctgc gagcggaccg ccgtccttcg cgagttcacc ggtggcgatg
     2161 gggaaccacg gaatgaaccc gatgccctcg tcgtcgcaca cgtcgacgag cgcctcggag
     2221 gcccggtcga tcaggttgta gcggttctgg accgtggcga tcgacgtgat ggcctgcgcc
     2281 gtgcgcagct cgtcgacgct gatctccgac agcccgatgt ggcggatctt gccttccgtc
     2341 tgcaggttga cgagcacccc gatctgctcc tcgacgggca cctgcgggtc gatgcggtgg
     2401 agctggaaca ggtcgatgcg ttcgagcccg agacggcgaa ggctcagctc cgcttcctga
     2461 cgcaggtact cggggcggcc gacgggccac cactcccccg gtccggtgcg cacgaggccg
     2521 gccttggtcg cgatcacgag atcgtcggcg taagggtgga gcgcctcgcg gatcagctcc
     2581 tcgctcacgt acggaccata ggagtcggcg gtgtcgatga agttcacacc cagctcgacg
     2641 gcacgccgca gcacggcgag tgcttcggcg tggtcgcggg gaggacccca cacgccggga
     2701 ccggtgatct gcatggcgcc gtagccgaga cggacgacgg ggaggtcgcc cccgatggag
     2761 aaggtgccgc tcgggacggc ggagatggtg gtgtcgctca tgggtcgggt caacccgctc
     2821 gacgggggat ctgttccggc cacgcccgac gacctcggcg gtcgacgacg tcagcgctcc
     2881 cgccggtctc ccccgatcgg ggcaccgacg cttggcgcag gtggtgagcg ggtatcggtc
     2941 cggcgtcccc caacaaggag tcgaatccca tgggcatgat ccgcaagacg atgtcgatcg
     3001 gcacgctcgg cctggtgtcg tggcgcagca aggccgagaa gctggagatc gccgagtacg
     3061 tgctcgctca ggcgcagtcc cgcaccgagg ccgagcacgc ggcccgcctc ggggccgaag
     3121 cgcgcgccga cctcgccacg gccgcactgg cccgctccga gaagcggacg cggcgccagc
     3181 gccgtcgcga ggagaccaag accctccttg ccaagagcag cgaggcggtc gaggagatcg
     3241 gccacgccgt cgcgcactcg gccaaggccg ccgagcccaa ggtgcggcgg gcccgccgtc
     3301 acgcccgacg gtccgccaag caggcccgcc gcacggcgac gtcgagagcc caggatctgg
     3361 ccggcaccgt gcgggaacag gcggaggccg ccaaggaaac cgtgctcgat caggccgagg
     3421 tgatccgcga cagggccgcg tcgctgaccg actgatcgac tgagccgagc ggagcggccg
     3481 acgaacgcgg ccgctccgct cgctcgctca ggacgtcttg accggccagg tgccgaggac
     3541 cgagctctcc tggttgccct cgccgaagtt gccggtgacc tcgacgacgt cgccgcagtg
     3601 gaacccgtca ccgtcgtcgt cgtcggagcc gacctcgggg tagaggcggg tccacgtgcc
     3661 gttctcgacg tggacgatca tcgagcagct cccgggcgtg ttggcgccgg ggtcacccga
     3721 tgagtgcaga ccgccgccgt cccagtcgtg cacctcgagg cccttctcca tgatgcactc
     3781 gcgggtgatc tcgccgtcct cggagcactg cttggccagg gtggcgaaga ggagcgtcga
     3841 cgagaacgcc tggatggcga gcacggcgtg ctcccagccg tcgacgttgg catccatgat
     3901 ctccatgaac tgcttcgtgg cgggccactc gtcggcctcc tcgaagggat gcatgttcga
     3961 ccggaccgtg acgccctcga cagcggccgg acccgacgcc gccagcaacc gcgagtcgta
     4021 ctggttggcg tcggccgtga tcacgccgtc ccacccctgg tccttcaggg cctgggagaa
     4081 cttcgacaga cccgacggtt cgccgatgta gctcaccagc tcgagaccct tgtcgatcac
     4141 ctgctgcgcg gtgacggcga agtcctggtt gatcgggtcg tagctgatct cgacgggatc
     4201 gccgtagccc tcgaccgcct tcacggtcgc cacggtctgg tccttgttct gcacgatcga
     4261 cgggagatcg ccgaacgcaa cgccgtactt ggcgatcttg tcggggtaca ccgacttcag
     4321 gccctcgaaa tggctgacgc ccttgaggtg cggcgggttg gggatcgcct ggatctggtc
     4381 ggacgcctcg gcgaattccg tcgacacggc gaagccggga acggccacca tgccgcactt
     4441 gtggaagtcg gagccgtcct tgccggtgaa gatgaggttg tcctgggcgt agccaccgcc
     4501 gaccatggcg aacacgccgg tgcacgcctt ggccatggcc gcttcgatct cgaacagctt
     4561 gccgtccatg tcgaccactt cgacgggcag gcccgccacg ccgccctgct cgttgcacca
     4621 cttcgcgaag cccgtggcac cgtcgaacag ctccttcagc aggccgttgc ggatcgacgt
     4681 gcggtcgttg gcgacgccga tgtagagctt gtcggcaccg gcgccggcct ggtcggcctt
     4741 gaccttcgcg tcaccgggac cacacgggct ctcgatcgac ccgaacttgc cggcaccgcc
     4801 gtcgggcgcc gacgagtcgg acccgccggc ggtcgtcgcc gtggtgccgt cggacgagga
     4861 cgacgtgtcc gccttgtccg agcccttcgc tcccccacag ccgctcgcga gcagtgcccc
     4921 caatacgacg aacaccgtca actgacgtct catctcgtcc cccccgggtc gatggatcga
     4981 acagcgaccc tatacccacc gacctccgga ccgtcggtca aaccgaccgg tggtcgatcc
     5041 cgctggccgg gacgaccgct agcttcggcc aatcgcaacg acacgatcgt ccatccaccg
     5101 ggcccgccgc cgtccggtca ccccgctgct cgtcctgtgc gccaccggag cgctcctgat
     5161 ctcggcatgc accgacgact cgaccgccga cgacgccccg gccgatcgat ccacgacctc
     5221 ggcggcgccc acgagcacga ccgctgccga gcgggtccgc ccggccggtc cggcagcgac
     5281 gttcgcgccg ctgaccggcg gcaagggccg cttcctcctg acggcgagca ccggtcccga
     5341 tctcgccgcc gccggctaca ccgagaccga gttcgcggcg tcggggacgg ccacggcgta
     5401 cacccaggac ggggagctgc ctgccgacgg caggctggcg ctgcgcccca cgaccagcgc
     5461 gccgtattcg acacgagtcg tcgtgcgtcg ccccgccacc gacgccgcct tcaacggcac
     5521 catggtcgtc gagtggctga acgtgtcgag cggagccgac gcttctcccg actacgccta
     5581 cctctccgac gagctcgtgc ggaaggggta cgcgtgggcg ggggtgtcgg cgcagcgcat
     5641 cggcatcgag ggcggcgacg ttgcggtgcc cgtctcgctc ggcatcgaca ccggtgccgg
     5701 caagggaatc cggacgatcg atccggagcg gtacggcgcg ctccgccacc ccggcgacgc
     5761 gtactcctac gacatcttca cccaggtcgg ccgcgccctc cgcggcgaca gcgccccggg
     5821 gccggcgacc gacggcctcg aggtcgatcg tgtgctcgcc gtcggcgaat cgcagtcggc
     5881 gttcgcgctg acgacctacg cgaacggtgt gcagcccctg accgacgcct tcgacgggtt
     5941 cctgatccac agccgtggcg gggcgggtgc gccgctcggc gcgcccgaca gcggcatcga
     6001 catcacctcg accgtcctcg gcccgcccac cctgctgcgc accgacctcg acgtgccgac
     6061 gatcgtcgtg cagaccgaga ccgatgtgct cggcatcctg aactactacc cctcccgcca
     6121 gcccgacacc gatcggctgc gggtgtggga ggtcgccggg accgcccacg cggaccggtt
     6181 ccagatcggc ccggtcgaat cggcgctcgg gtgtgccggg ccgatcaaca acggccagca
     6241 ggcgttcgtg ctgcgtgctg cgttgcggga tctcgacgag tgggcggccg gcggtgatgc
     6301 cccaccgacc gccgaaccgt tgacgatcga ggagtccgcg ggctcgccgg agttcgtgct
     6361 cgacgaggtc ggcaacgtca cgggcggggt ccgcactccc gcggtcgagg cgcccactgc
     6421 ggtgctctcg ggcctgccgg cggacggggc gtcgatcgtc tgcatcctca tgggttcgac
     6481 ctcgccgttc cccgcggacc agctcgcggc gcggtacccc gggcccgacg actacgccac
     6541 tgcctgggcg gcggcgacgg acgcgaccat cgacgccggg ttcttcctgc ccgaggaccg
     6601 cgctgcgatc atcgccgacg cccggcccac cggactcgcc gccggctgat cggtcggacc
     6661 atcagaccat cagaccatca gaccatgtcg gtcgggcgga gaccgaaccc gagcgcgtgc
     6721 cgaccggcaa gctcggcggt gcggtcccag tcgaacatca cgtggccctt caacgtctcg
     6781 acgtcgcgct gcaggcgctg gaggggatgt gaggagaagt acacgctggc cccggcacca
     6841 tcgcacacgt cggtgacgat gcggcgagcg ctgcggaccg tcgcggcggc agcgaggcgc
     6901 gccgacaccc ggagctcctc ggtgacggcg cccgctgcgc cgccgcgctc gaccgccgcg
     6961 atcgaggctt cccagtggtg gcgggccgcg gcgacgtcgc tcgtcgccgt ggcgagccgg
     7021 acctgtgctg cgggctgctc ggcggcctgg tcgcccagcg tgtaggcgag cacgcgcgtc
     7081 gccagtcgct cgcggtacat gccgacggcc gcctcggccg cgcc
//
LOCUS       DAOSOA010000151         7090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10634_length_7090_cov_5.915281, whole genome shotgun sequence.
ACCESSION   DAOSOA010000151 DAOSOA010000000
VERSION     DAOSOA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10634_length_7090_cov_5.915281"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKY13_10155"
     CDS             <1..408
                     /locus_tag="PKY13_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80340.1"
                     /translation="PALRALRCEVARWALAAGQPLNLDAIGVILGARHAEAIVEGRPF
                     NRWTTNTVLTFLFGTAEDWCLRQHVTMPPHLGESLLTYVNFLAAAGVLAPGSSSVRQL
                     QNTISDLAGLTATGHRRPSRVDDGAPTPTPLRR"
     gene            434..1864
                     /locus_tag="PKY13_10160"
     CDS             434..1864
                     /locus_tag="PKY13_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT80341.1"
                     /translation="MPLPHRSTEYDATIVGTGPNGLVAAVTLARAGWRVLAVEAAPTP
                     GGGTRSAELTRPGLVHDVCSAIHPLGVASEAMRNLPLADHGLEWVQPEIAAGHPLDGG
                     RAGLLYPSLDRTVAGLGTDGDAYRRLLAPLVRQDLVDSLLSPLSLPSAPVALARYGLA
                     GILPLDRLARSRFSTDEAKALLAGLAAHSMLSLRSPITAGYGLMLGLLGHLVGWPMAK
                     GGSQAIADALVSLLEAHGGTVECGRPIRSLDELPPSRATIFDVTPTQLVAICGDRMPD
                     RYRRRLTSFRRGPGVWKVDWALDGPVPWTNPDLHRTATVHLGGTLEEIVEAEDEVIAG
                     RHPERPYVLYVQTSQFDRSRAAAGGETGWAYCHVPNGSTVDMTARIEAQIERFAPGFR
                     DRIVERHVMSPAAMQAHNANYLGGDINGGMANLRQFVARPTLSLHPWRTPMPGIYLCS
                     SSTPPGGGVHGMCGLHAARAALSDLG"
     gene            complement(1910..2923)
                     /locus_tag="PKY13_10165"
     CDS             complement(1910..2923)
                     /locus_tag="PKY13_10165"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOT80342.1"
                     /translation="MQPFSTDDSDGRAWTLPPPPPPRKRRRWLWGCLPALVIGVVLLI
                     IAALLVARQQDGAIDRSPDGTVLPPNTVTTPVPGSTAVTSSCAITQGLITRECAVILP
                     PGVPEGTKLPVVFLLHGLGDGPGTIRDAGAWTDAVLARRFMLVTPSGIASSWNAGDCC
                     GIAKGTGIDDTSYLATLVEEIARRPDVDTSRMYMAGFSNGGMMAYRFACVSDRLAGIA
                     SVSGTRVINCPPRQPLPILHIHGTADDTVPYQGGAGLVAAIMGVSFQPVPTMAEQVAA
                     DDGCTAPPSDRTDGDLTYEEWNGCGGGSRVELITIANWGHLWPLTGSLDATDEILSFF
                     GIS"
     gene            3036..4232
                     /locus_tag="PKY13_10170"
     CDS             3036..4232
                     /locus_tag="PKY13_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80343.1"
                     /translation="MQLGDSQADAEFRAEIRDWMAANLTGEFEQLRFRGGPGDEHMFV
                     EERMAWERHLAAHRWTCVGWPAEHGGRGLPISQEVIFHEEYARAGGPGRAGLIGEGLL
                     GPTLIHFASPEQQQRFLPGIVAGTEYWCQGYSEPNAGSDLANIKTTARLEGEGDDAEW
                     VINGQKVWTSLAQWSDWCFVICRVEEGSQRHAGLAYLLVPMDADGIEVRPIRQITGTA
                     EFNEVFFTDVRTAAHNIVGEPGEGWKVAMGTLAFERGVLTLGQQMAFQRELDEIIETA
                     RHNGRIDDPILRDRLVGLWIRLRIMRTNAIRTLSLASAQLGREAMIGKLYWANLHQEM
                     GEVAMDVLGPEAMVIADRYDAHEPLDALHRLFMFTRSDTIYGGSDQIQRNVIGERALG
                     LPREPR"
     gene            4259..5041
                     /locus_tag="PKY13_10175"
     CDS             4259..5041
                     /locus_tag="PKY13_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT80344.1"
                     /translation="MAAPVPTYPAAHGLLAGKTVVVTAAAGTGIGFSLAKRCVEEGAT
                     VLISDLHERRLGEAADNIEAETGTRPATFLCNVTDEEQVQGLVAASIEALGHIDVWMN
                     NAGLGGNAAVVDMTDDQWNVVLDVTLTGTFRCLRAVLPHMYERGTGAIVNNASVLGWR
                     AQELQAHYAAAKAGVMALTRCTAMEAAKHNVRVNAVSPSLAMHPFLAKVTPDGLLEEL
                     EAREAFGRAAEPWEIANVMVMLASDYTTYMTGEVVAVSSQHA"
     gene            5092..5454
                     /locus_tag="PKY13_10180"
     CDS             5092..5454
                     /locus_tag="PKY13_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80345.1"
                     /translation="MKLRRSVAVVAGSVLLLSACGGEELPSKSEFISKLKQEMSSEMS
                     SLESSGIDKAAAEKIFDDFLGCTYDEIKGDTELLSRVNDGADDAATTAEITKKATACA
                     NDLQQAAVEAATGGSAGG"
     gene            5447..5818
                     /locus_tag="PKY13_10185"
     CDS             5447..5818
                     /locus_tag="PKY13_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80346.1"
                     /translation="MADLRRRAAVAAVALSLVATGCGGPPTKAEFVTKMRESVGDLDA
                     GLASQDIDPETGNELITKLLECQYESIKDDDDLLQKAYDDPGDTEIAPQLDAKTTACV
                     DDFSTAMADLAGPTTTTIPEG"
     gene            5824..6282
                     /locus_tag="PKY13_10190"
     CDS             5824..6282
                     /locus_tag="PKY13_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOT80347.1"
                     /translation="MSLSVPTPADLLEHVGDSLGPTEWVVVDQERINQFAEATGDHQW
                     IHVDVERATAESPFGGPIAHGYLTLALANLFLPQLLSVETFSAGLNIGLDKVRFPSPV
                     PAGSKIRGSGEIVSAVEAGGGVQVVVRVTIEAEGATKPACVADTVSRFLP"
     gene            6449..>7090
                     /locus_tag="PKY13_10195"
     CDS             6449..>7090
                     /locus_tag="PKY13_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOT80348.1"
                     /translation="MPEAVIVATARTPIGRAMKGSLVDMRPDDLTAQIVNAVFEKVPS
                     LDRNLVEDLILGCGQPAGESGVNLARVVAVLSDMPNVPGVTINRYCSSSLQAIRMAGH
                     AIKAGEGDIFLAAGVETVSRFINGMADDGPANPKFDDARARTSARQAGDQGDWTAPVG
                     LPDVYMGMGDTAENVAEQEKVGRQEQDEFAARSQQLATESLENGFWAAEITPVT"
BASE COUNT         1092 a         2608 c         2357 g         1033 t
ORIGIN      
        1 ccggccctgc gcgccctccg ctgcgaggtc gcccgttggg ccctcgccgc cgggcaacca
       61 ctcaacctcg atgccatcgg ggtcatcctc ggggctcggc acgccgaggc gatcgtcgag
      121 ggccggccgt tcaaccgctg gaccaccaac acggtgctca cgttcctctt cggcaccgcc
      181 gaggactggt gccttcgcca gcacgtcacg atgccgcccc acctcggcga gtcgctcctg
      241 acctacgtca actttctcgc cgccgcgggg gtgctcgccc ccggctcgtc gtcggtccgc
      301 cagctgcaga acacgatctc cgacctcgcc ggcctcacgg cgaccggtca tcggcggccg
      361 tcccgggtcg acgacggcgc acccactccc acgccgcttc gacgctgacg cgatcggggt
      421 agcgtccccg ccgatgcctc tgccccaccg atcgaccgag tacgacgcca ccatcgtggg
      481 caccggcccc aatgggctgg tcgccgccgt caccctcgct cgcgccggat ggcgcgtgct
      541 cgcggtcgag gccgctccca ctcccggggg cgggacgcgc tcggccgagc tgacccgacc
      601 gggtctcgtg cacgacgtgt gttccgcgat ccatcccctc ggcgtcgcgt ccgaggcgat
      661 gcgcaacctg ccgctcgcgg accacggcct cgaatgggtc caacccgaga tcgccgccgg
      721 ccacccgctc gatggcggcc gggcggggct cctgtacccg tcgctcgacc ggacggtcgc
      781 cggtctcggc accgacggcg acgcctaccg ccgcctcctc gccccactcg ttcgtcagga
      841 cctggtcgac tcgctgctgt cgcccctgtc gcttccctcg gcaccggtgg cgctcgcacg
      901 gtacgggctg gccggcatcc tgccactcga tcgcctcgcc cgctcccgct tctcgaccga
      961 cgaggccaag gcgctgctcg ccgggctcgc cgcccactcg atgctgtcgc tgcggtcgcc
     1021 catcaccgcg gggtacggcc tgatgctcgg cctgctcgga catctcgtgg gctggccgat
     1081 ggcgaagggc gggtcgcagg cgatcgccga cgcgctggtg tcgctgctcg aggcccacgg
     1141 cggcaccgtc gagtgcgggc gtcccatccg ctcgctcgac gagctcccgc cgtcgcgggc
     1201 aaccatcttc gatgtcacgc cgacccaact cgttgcgatc tgcggcgacc ggatgcccga
     1261 tcgttaccgc cgtcggctca cgtcgttccg acgcgggccg ggcgtgtgga aagtcgactg
     1321 ggcactcgac gggccggtcc cgtggaccaa ccccgacctc caccgcaccg cgacggtgca
     1381 cctcggcggg acgctggagg agatcgtgga ggccgaggac gaggtgatcg ccggccgcca
     1441 tcccgaacgc ccctacgtcc tctacgtgca gacgagccag ttcgaccgga gccgcgccgc
     1501 cgccggcggc gagacagggt gggcgtactg ccacgtgccc aacgggtcga cggtcgacat
     1561 gaccgcgcgg atcgaggcac agatcgagcg cttcgccccg ggcttccgcg accgcatcgt
     1621 cgaacgccac gtgatgagcc ccgccgcgat gcaggcgcac aatgccaact acctcggcgg
     1681 cgacatcaac ggcggcatgg cgaacctccg ccagttcgtc gcccggccga cgctgtcgct
     1741 ccacccgtgg cgcaccccga tgccgggtat ctacctgtgc tcgtcgtcga caccgcccgg
     1801 cggcggcgtc cacgggatgt gtggcctcca cgcggcccga gccgcgctgt ccgacctcgg
     1861 ctgaggatcg cgtcgatctc gtcgccggcg cggcttgggc gccgcggcgc tagctgatgc
     1921 cgaagaacga gaggatctcg tcggtcgcgt cgagggagcc ggtgagcggc cacagatggc
     1981 cccagttcgc gatcgtgatg agctcgacgc gcgatccccc gccgcagccg ttccactcct
     2041 cgtaggtgag gtcgccgtcg gtgcggtcgc tgggcggagc ggtgcagccg tcgtcggcag
     2101 cgacctgttc ggccatcgtg ggcaccggct ggaagctcac tcccatgatc gccgcgacca
     2161 gcccggcacc gccctggtac ggcacggtgt cgtccgccgt gccgtgaatg tggaggatgg
     2221 ggagcggttg tcggggcggg cagttgatca cgcgtgttcc cgagacgctg gcgatgccgg
     2281 caagtcgatc gctgacgcac gcgaaccggt aggccatcat gccgccgttc gagaaccccg
     2341 ccatgtacat gcgactggtg tcgacgtcgg gccggcgggc gatctcctcc accagggtcg
     2401 cgaggtagct ggtgtcgtcg ataccggtgc ccttggcgat gccgcagcag tcaccggcgt
     2461 tccaactgct cgcgatgccc gacggcgtga cgagcatgaa ccttcgggcg aggacggcgt
     2521 cggtccacgc tccggcgtca cgaatcgtgc ccgggccgtc accgagcccg tgcagcagga
     2581 agaccaccgg cagcttggtt ccctcgggca cgcccggcgg gaggatcacc gcacattccc
     2641 gggtgatcag gccttgggtg atcgcacaac tcgaggtgac ggccgtgctg cccggcaccg
     2701 gggtcgtcac ggtgttcggc ggcagcaccg tgccgtcggg gctgcggtcg atcgccccgt
     2761 cctgctgccg ggcgacgagc agcgcggcga tgatgagcaa cacgacgccg atgaccaggg
     2821 cggggagaca cccccacagc catcgacgcc gcttcctggg cggcgggggt ggcgggaggg
     2881 tccacgccct tccgtcggag tcatcggtcg aaaagggctg caaggcggga catggtaggc
     2941 ggggcctcga tccctccccc atcaggtcct gattccgctc cggccgccgt gcccgattcc
     3001 gagatctgac gaggcgtcag atacgatcga ccaccatgca gttgggagat tcgcaggccg
     3061 acgccgagtt ccgcgccgag atccgtgact ggatggcggc gaacctcacc ggcgagttcg
     3121 agcagctccg cttccgcggc ggccccggcg acgagcacat gttcgtcgag gagcgcatgg
     3181 cctgggagcg ccacctggcg gcgcaccggt ggacgtgcgt cggttggccg gccgagcacg
     3241 ggggccgcgg gctgccgatc agccaggagg tcatcttcca cgaggagtac gcccgcgcgg
     3301 gtggccccgg acgcgccggc ctcatcggcg agggcctgct cggcccgacc ctcatccact
     3361 tcgcgtcgcc cgaacagcag cagcggttcc tccccggcat cgtggcgggc accgagtact
     3421 ggtgccaggg ctactccgag cccaatgccg gcagcgacct cgccaacatc aagacgaccg
     3481 cccgcctcga gggcgagggc gacgacgccg aatgggtgat caacggccag aaggtctgga
     3541 cgtcgctcgc gcagtggtcc gactggtgct tcgtcatctg ccgggtcgag gagggcagcc
     3601 agcgccacgc cggcctcgcc tacctgctgg tgcccatgga cgccgacggc atcgaggttc
     3661 gcccgatccg ccagatcacc ggcaccgccg agttcaacga ggtgttcttc accgacgtcc
     3721 gcaccgcggc ccacaacatc gtcggcgagc ccggcgaggg gtggaaggtc gcgatgggca
     3781 cgctcgcgtt cgaacgtggg gtactcacgc tcggccagca gatggcattc cagcgtgagc
     3841 tggacgagat catcgagacc gcccggcaca acggtcgaat cgacgacccg atcctgcgag
     3901 accgtctcgt cggcctgtgg atccgcctgc ggatcatgcg caccaacgcg attcgcaccc
     3961 tgtcgctggc gtcggcgcag ctcggccgcg aagcgatgat cggcaagctc tactgggcga
     4021 acctccacca ggagatgggt gaggtcgcga tggacgtcct cggtcccgag gccatggtca
     4081 tcgccgaccg ctacgacgct cacgagcccc tcgacgcgct gcaccgtctc ttcatgttca
     4141 cgcggtccga caccatctac ggcgggtccg accagatcca acgcaacgtc atcggcgagc
     4201 gggcgctcgg cctgccgcgc gaaccccgct agcagtccca ccgaccttca ggagcaccat
     4261 ggccgcaccc gttcccacct atcccgctgc gcacgggctg ctcgccggca agaccgttgt
     4321 ggtcaccgcc gccgcgggca ccggcattgg tttctcgctg gccaagcgct gcgtcgaaga
     4381 gggcgcgacc gtgctgatca gcgatctcca cgagcgtcgc ctgggcgagg ccgccgacaa
     4441 catcgaggca gagacgggca cccgtccggc gacgttcctc tgcaacgtca ccgacgagga
     4501 gcaggtgcag gggctggtcg cagcgtcgat cgaggcgctc ggccacatcg acgtgtggat
     4561 gaacaacgcc ggcctcggag gcaacgccgc agtcgtcgac atgaccgacg accagtggaa
     4621 cgtcgtgttg gacgtgaccc tcaccggcac gttccgttgc ctgcgggccg tgctccccca
     4681 catgtacgag cgaggcacgg gcgcgatcgt caacaacgcg tcggtcctcg gatggcgggc
     4741 gcaggaactg caggcgcact acgccgcagc gaaggccggc gtgatggccc tgacccgctg
     4801 caccgcgatg gaagcggcca agcacaacgt ccgggtgaac gcggtgtcgc cgagcctggc
     4861 gatgcacccg ttcctcgcca aggtgacgcc cgacggcctg ctggaggagc tcgaggcccg
     4921 tgaagcgttc ggccgggccg ccgagccgtg ggagatcgcc aacgtcatgg tcatgttggc
     4981 gtcggactac acgacctaca tgacgggcga ggtcgtcgcc gtgtcgagcc agcacgcgtg
     5041 agggccggtg gccctacact cgcgcgtcgc cgccaaccga aaggcccgcc catgaagctc
     5101 cgccgatccg tcgccgtcgt ggctgggtcc gtcctcctgc tctccgcctg cgggggtgag
     5161 gaactgccct cgaagagcga gttcatctcc aagttgaagc aggagatgtc gtcggagatg
     5221 tcgtcgctcg aatcctcggg catcgacaag gccgccgccg agaagatctt cgacgacttc
     5281 ctcggctgca cctacgacga gatcaagggg gacaccgagc tgctgtcccg ggtcaacgac
     5341 ggtgccgacg acgctgcgac caccgccgag atcacgaaga aggcaacggc gtgtgcgaac
     5401 gacctgcagc aggcggcggt cgaggccgcc accggaggct cggccggtgg ctgacctccg
     5461 acgacgcgcc gcggtcgcag ccgtcgccct ctcactcgtc gcaaccgggt gcggcggtcc
     5521 gcccaccaag gccgagttcg tgacgaagat gcgcgagtcc gtcggtgacc tcgatgccgg
     5581 gctcgcgagc caggacatcg accccgagac gggcaacgag ctgatcacga agctgctcga
     5641 gtgccagtac gagtcgatca aggacgacga cgacctgttg cagaaggcgt acgacgatcc
     5701 gggcgacacc gagatcgcac cgcagctcga tgccaagacg acggcctgcg tcgacgactt
     5761 ctcgacggcg atggccgacc tcgccggtcc gaccaccacc acgatccccg aaggctgagc
     5821 ccgatgtcgc tgtccgtccc cacgcccgcc gacctgctcg agcacgtcgg cgactcgctc
     5881 ggccccaccg agtgggtggt cgtcgaccag gagcgcatca accagttcgc cgaggcgacc
     5941 ggcgaccacc agtggatcca cgtcgacgtc gagcgagcga ccgccgagag tcccttcggc
     6001 gggccgatcg cccacggcta cctgacgctc gctctcgcca acctgttcct gccgcagctg
     6061 ctgagcgtcg agacgttctc cgccgggctc aacatcggcc tcgacaaggt ccgcttcccg
     6121 tcgccggtcc cggccggctc caagatccgc gggagcggcg agatcgtgtc cgccgtcgaa
     6181 gccggtggcg gcgtccaggt ggtcgtacgg gtcacgatcg aggcggaggg cgccaccaag
     6241 cccgcgtgtg tcgccgacac cgtctccaga ttcctgccct gaaccgcctg ccctgatctc
     6301 accggttctg tcctgcgctt ccgcccctcc aggggcggat ctgcaggaca gaacgggtgc
     6361 atctctcggt tcgctcgccg ttgttaccgg ccggtaacat gcggcaccga caatcgccac
     6421 ccacccaacc caacgggagc caagtccaat gcctgaagca gtcatcgtcg ccaccgcccg
     6481 cacgcccatc gggcgggcca tgaagggttc gctggtcgac atgcgtcccg acgacctcac
     6541 cgcgcagatc gtcaacgccg tcttcgagaa ggtgccgtcg ctcgaccgca acctcgtcga
     6601 agacctcatc ttgggctgcg gccagcccgc cggtgagtcc ggcgtgaacc tcgcccgcgt
     6661 cgtcgcggtg ctgtccgaca tgccgaacgt ccccggcgtc accatcaacc gctactgctc
     6721 gtcgtcgctc caggcgatcc gcatggccgg ccacgccatc aaggccggcg agggcgacat
     6781 cttcctcgct gccggcgtcg agaccgtcag ccgcttcatc aacggcatgg ccgacgacgg
     6841 ccccgccaac ccgaagttcg acgacgcccg cgcccgcacc tccgcccgcc aggccggcga
     6901 ccagggcgat tggaccgcgc cggtcggcct gcccgacgtc tacatgggca tgggtgacac
     6961 cgccgagaac gtcgccgagc aggagaaggt cggccgccag gagcaggacg agttcgccgc
     7021 ccgctcccag cagctcgcca ccgagtcgct cgagaacggc ttctgggccg ccgagatcac
     7081 ccccgtcacc
//
LOCUS       DAOSOA010000152         7060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10696_length_7060_cov_6.766310, whole genome shotgun sequence.
ACCESSION   DAOSOA010000152 DAOSOA010000000
VERSION     DAOSOA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_7060_cov_6.766310"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PKY13_10200"
     CDS             complement(<1..698)
                     /locus_tag="PKY13_10200"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HOT80349.1"
                     /translation="MTATTPSPDTTTAYTDGACIGNPGPGGWAWAVPGGAFASGSAAA
                     TTNQRMEVQAALEAIRAIEGPLRIVSDSKYVVDCFKQRWYDGWERRDWKNAKKEPVKN
                     QDLWKPLIGLYLARRDEITFEWVKGHSGDEMNDVVDRLAVEAARTQGARSGGEPPTDL
                     GEADVVDTGPAPSTASSGIAGWKVAAFGHRPPQLGGYDVDNPTAADVRSRLRDVLRAW
                     RQIHPDLVVLTGLGL"
     gene            833..3619
                     /locus_tag="PKY13_10205"
     CDS             833..3619
                     /locus_tag="PKY13_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT80350.1"
                     /translation="MTLLVGLHFVVGLAIVALGSRVDRRALLVGALAPAAVVIWLVTR
                     IGDLGAGRVVEESTSWAPGLGLSVDLRLDGFAALMVCLVSGLGVVVLVYGYGYFPQRR
                     EGLGRLTGLMLCFSGSMLGLVLADNLLVLATFWELTAVTSFLLIGNDHTSQAARAAAL
                     QAILTTGLGGLAMLGGFIVIGNEAGTYRLSSILASPPTGTAVTVGLMLVLLGAVTKSA
                     QYPFHSWLPGAMVAPTPVSAYLHSATMVKAGVYLVARLALPFASAPGWRPTVVTIGLV
                     TMLAGGLRAMRQTDLKLLLAFGTVSQLGFMFVLFGAGTPGTVEAGCVLLVAHGLFKAG
                     LFMGVGVLDHQLGTRDVSQLPALGPGWRTARAVMVASAASMAGLPLVLGFIAKEAAYE
                     SLLHDETFGGRVTLVGVVLGSVLTLAYSARFVWGAFLAPRRRGVHEAAASTGPSAPFL
                     APVVVVTALTVVFGLVPALLDGLAGRAAGSLRPGEGTTGVHLAIWHGVGAALLLTVIT
                     YALGAALHRGSVPVARILGRGSALPSGTVVYRSVLRSMNRLADLVTGTVQNGSLPVYA
                     GVVLLTAAAFPVVALVRGAPAPDRPPWIDGPGQAALVALVLGAALGAASARRRFAAAL
                     FLGLAGYSMAGLFVLQGAPDLALTQVAIETLTTVLFVLVLRRLPDRFERVGAIAGRPA
                     RVAIAASVGLMVFVFALVAASERVGPPVSEEMTARALPDGGGRNVVNVILVDFRGFDT
                     LGEITVLAAAAIGAVALARAGRRPGAPATESPAEVRRVFHLDQSVSVIFYVMLAASIY
                     LLFAGHNQPGGGFVGGLVAGAAVALRYVAGGIDEVRRVSPVKPWTILGSGVVASAACA
                     LVPVLFGNPVLDNGYVNADLPGIGKMKLSSAIVFDFGVYLVVIGLVLMVVEAFGERFA
                     PAPAPAQVAKGEPT"
     gene            3616..4059
                     /locus_tag="PKY13_10210"
     CDS             3616..4059
                     /locus_tag="PKY13_10210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOT80351.1"
                     /translation="MTVALAAAAALLFAIGTYLVLQRRLSRIIIGIGLMGHGANVLFL
                     VSGRRGDPPLVGSAPASDFSDPLPQAMVLTAIVITFGVTALLLALAYRSWLLTDDDEV
                     EDDVSDRVIAGGGFIDREVTDADAALADVIDPAGGHRATNRRRRR"
     gene            4056..5549
                     /locus_tag="PKY13_10215"
     CDS             4056..5549
                     /locus_tag="PKY13_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HOT80352.1"
                     /translation="MTALLTLPILLPLIGSGCSMIVGRSRVLQRIVSLTVLSAMAAVS
                     IGLLVVVDRDGPIAVQAGGWAAPMGITLVADRLSALMLAVASVMLLAVLVYAIGQPGA
                     ERNHVGFQSVYLVLSAGVAGAFLTGDLFNLFVSFEMMLAASYVLITLGARRDQVRAGM
                     TYVVISLIASTLFVTALALVYSATGTVNLADLSQRMEQLSPGLRTAFSVLLLVVFGIK
                     AGLFPLFFWLPDSYPTAPSAVTAVFAGLLTKVGVYAIIRTQTLLFPPESRPSTLILVL
                     AGATMVVGVLGAIAQDDVKRILSFHIVSHVGFMVMGLGFFTVAGLGGAVFYIVHHIVV
                     MTTLFLTGGLIEHVGGSSRLSRVGGLVTGAPVVAVLFLVPALSLAGVPPFSGFVAKLG
                     LLQAGVSASHGVIVGVALVVSLLTLFSMMKIWSAVFWGEKPSEPAVSLGTAPPLMVAP
                     TLVLAVVSLVIAVSAGPLHDLGVRAATGLLDPHEYLEAVRQPEGLAR"
     gene            5546..6076
                     /locus_tag="PKY13_10220"
     CDS             5546..6076
                     /locus_tag="PKY13_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOT80353.1"
                     /translation="MSRLTLGVCIVIGWMFLWGDVGVGPVIGGTLVVVTLYVVFPSSR
                     ASFPRVVVHPFAVARLFAYFVRQLVVSNALLTRELLRRRNSICTRVVEIPMRTSVPSL
                     LTMVTNLASLSPGTIVVDADELAPGTPPTLVVHVLMFGDLDDPEAAVRSVHALEERCV
                     LAFGTNDAIRELELAR"
     gene            6073..6351
                     /locus_tag="PKY13_10225"
     CDS             6073..6351
                     /locus_tag="PKY13_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOT80354.1"
                     /translation="MTVVTMLVLAAAAAAYLVRLVRGPGLSERVIALDGLIVVGISAV
                     AVHAMSTGQGAFLPVLVVITLVGFVATAAAARFIERTDGVDQRPGDAR"
     gene            6348..6653
                     /gene="mnhG"
                     /locus_tag="PKY13_10230"
     CDS             6348..6653
                     /gene="mnhG"
                     /locus_tag="PKY13_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOT80355.1"
                     /translation="MIGQLLALGGAVLILFSALGIVRFDDVFAQMHFLTKASTLGVVL
                     VLGGAALVMERANDWSSLLLAAFLQLLTSPVAANLIGRSTYLTRVRPIQGSTTTPVE"
     gene            6650..>7060
                     /locus_tag="PKY13_10235"
     CDS             6650..>7060
                     /locus_tag="PKY13_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80356.1"
                     /translation="MSAGIDEPGGQVLQAGQDIETVPGWTSLIAGEPWTASELWLSAA
                     SIDIRRVAPRWSGAAEPSAGLELSGPPELTSIGLADVVGVDHIGDDPDGLAILEFALA
                     AGNVVHARLPESFVDEVVAALQRSRAGVPPTFEPA"
BASE COUNT          792 a         2413 c         2510 g         1345 t
ORIGIN      
        1 aggcccagtc cggtgagcac cacgagatcg ggatggatct ggcgccatgc ccgcagtacg
       61 tcgcggaggc gactccggac gtcggctgcc gtcgggttgt cgacgtcgta gccgccgagc
      121 tgcggagggc ggtgaccgaa ggccgcgacc ttccaaccgg cgatgcccga tgatgctgtt
      181 gagggcgccg ggcccgtgtc gacaacgtcg gcctcgccca ggtccgtggg cggttcgccg
      241 cccgaccggg cgccctgggt ccgggccgcc tcgacggcga ggcggtcgac cacgtcgttc
      301 atctcgtcgc cgctgtggcc cttgacccac tcgaacgtga tctcgtcgcg tcgggcgaga
      361 tacaggccga tcagtggctt ccacaggtcc tggttcttga ccggttcctt cttcgcgttc
      421 ttccagtcgc gccgctccca gccgtcgtac cagcgctgct tgaagcagtc gacgacgtac
      481 ttggaatcgc tgacgatgcg gaggggtccc tcgatggcgc ggatcgcctc gagcgccgcc
      541 tgcacctcca tccgctggtt cgtggtggca gccgccgaac cgctcgcgaa cgccccgccc
      601 ggcacggccc atgcccatcc gccggggccg gggttcccga tgcaggcgcc gtcggtatag
      661 gcggtcgtgg tgtcggggga aggagtcgtg gcggtcatcg gcatcgacgt taccgatccc
      721 agtctgaggc gttcggcccc taacctgccc cgatgggtcg gtcgacagtg cggttcggtg
      781 acgtctcgcc gcctgcgatg ggcccggtcg gcgattggga ggagtcatct cgatgacgct
      841 cctggtgggc ctccacttcg tggtgggact cgccatcgtc gctctgggga gtcgcgtcga
      901 tcggcgggct ctgctcgtcg gcgccctcgc cccggcggcg gtcgtcatct ggttggtgac
      961 acgaatcggc gacctcggag cgggtcgggt cgtggaggag tcgacgtcgt gggcccccgg
     1021 actcgggctg tcggtcgacc tacggctcga cgggttcgcc gcgctcatgg tgtgcctcgt
     1081 gtcgggcttg ggcgtcgtcg tactggtcta cggctacggg tacttccccc agcggcggga
     1141 gggcctcgga cgattgaccg gcctcatgct gtgcttcagc ggttcgatgc tcgggctggt
     1201 actcgccgac aacctgctcg tcctcgcgac gttctgggag ctcaccgcgg tcacgtcgtt
     1261 cctgttgatc ggcaacgacc acacgagcca ggccgcccgc gccgccgcgc tgcaggcgat
     1321 cctgacgacc gggctcggcg gcctcgcgat gctcggtgga ttcatcgtga tcggcaacga
     1381 ggcgggcacc taccggctga gctcgatcct cgcctcgccg ccgacgggca cggccgtcac
     1441 cgtcgggctg atgctggtgc tgctcggcgc cgtcaccaag tcggcgcagt acccgttcca
     1501 ctcgtggttg ccgggagcga tggtcgcgcc gacaccggtg agcgcgtacc tgcactcggc
     1561 gacgatggtg aaggccgggg tgtacctggt ggcccgcctg gcgctgccgt tcgcctccgc
     1621 tcccggctgg cgcccgacgg tggtcaccat cgggctcgtc acgatgctgg ccggtgggct
     1681 ccgggcgatg cgtcagaccg acctcaagct cctccttgcg ttcggcaccg tgagccagct
     1741 ggggttcatg ttcgtgctct tcggcgccgg cacacccgga accgtcgaag cagggtgtgt
     1801 cctgctggtc gcccacggcc tgttcaaggc cggcctgttc atgggggtgg gggtgctcga
     1861 ccaccagctc ggcacccgcg acgtgtccca gctgccagcg ttgggcccgg gctggaggac
     1921 agcgcgggcg gtcatggtcg cgtccgccgc gtcgatggcg gggctgccgc tcgtcctcgg
     1981 tttcatcgcc aaggaggccg cgtacgagtc gttgctccac gacgagacct tcggcggtcg
     2041 ggtcacgctc gtgggcgtgg tgctgggctc ggtgctcacc ctcgcgtaca gcgcccggtt
     2101 cgtctggggt gcgttcctgg cgccccgacg ccggggagtt cacgaggcgg cggcgtcgac
     2161 cggcccgtcg gccccgttcc tcgctcccgt ggtcgtggtc acagcgctga ccgtcgtgtt
     2221 cgggctggtg cccgccctgc tcgacggcct cgccggtcgg gccgccggct cgctccgtcc
     2281 gggcgagggg acgaccggtg tccacctggc gatctggcac ggcgtcggtg ccgcactgtt
     2341 gttgacggtc atcacgtacg cgctcggcgc cgcgttgcac cgtgggtcgg ttcccgtcgc
     2401 gcgaatcctc ggccggggga gcgcgctccc gagcggaacg gtcgtgtacc gctcggtctt
     2461 gcggtcgatg aaccgcctcg ccgacctggt cacgggcacc gtgcagaacg ggtcgctccc
     2521 cgtgtacgcg ggggtggtgc tgctcacggc agcagcgttc cccgtcgtgg cgttggtgcg
     2581 aggtgccccc gcgcccgacc gcccgccgtg gatcgacggc ccgggtcagg cggccctcgt
     2641 cgcgctcgtg ctgggggctg cgctcggcgc ggcgtcggca cgccgtcgct tcgccgcggc
     2701 cttgttcctc ggcctggccg gctactcgat ggcgggcctg ttcgtcctcc agggggcgcc
     2761 cgaccttgcc ctcacacagg tggcgatcga gaccctgacc acggtgctct tcgtcctcgt
     2821 gctgcgacgc ctgcccgacc ggttcgagcg cgtcggcgcg atcgccgggc gtcccgcccg
     2881 cgtcgccatc gccgcatcgg tcggcctgat ggtgttcgtg ttcgcgctcg tcgcggccag
     2941 cgagcgggtc ggcccaccgg tctcggagga gatgacggcg cgtgcgcttc ccgacggcgg
     3001 ggggcgcaac gtcgtcaacg tgatcctcgt cgacttccgg gggttcgaca ccctggggga
     3061 gatcacggtg ctggccgccg cagcgatcgg cgcggtggcg ctcgcccgtg ccggccggcg
     3121 ccccggcgct cccgcgaccg agtcgccggc cgaggtgcgg agggtgttcc acctcgacca
     3181 gtcggtgtcg gtgatcttct acgtgatgct cgccgcctcg atctacctgt tgttcgccgg
     3241 tcacaaccag cccggtggcg gattcgtcgg cggtctcgtc gccggggctg ccgtcgcgct
     3301 ccggtacgtg gccggcggca tcgatgaggt ccggcgggtc agcccggtca agccgtggac
     3361 gattctcggt tcgggcgtcg tggcctcggc ggcatgtgcg ttggtcccgg tcctgttcgg
     3421 caacccggtg ctcgacaacg ggtacgtgaa cgccgatctg ccgggcatcg ggaagatgaa
     3481 actgtcgtcg gcgatcgtgt tcgacttcgg tgtctacctg gtggtgatcg ggctggtgct
     3541 catggtcgtg gaggccttcg gcgagcgctt cgcccccgcc ccggccccgg cccaggtcgc
     3601 caagggggaa ccgacatgac cgtggcgttg gctgcagcgg ccgccctcct cttcgccatc
     3661 ggcacgtacc tggtgctgca gcgacggctc agtcggatca tcatcgggat cggcctcatg
     3721 ggacacggcg ccaacgtgct gtttctcgtg tcgggccgac gcggcgatcc gccgctcgtg
     3781 gggtcggcgc cggcctcgga cttcagcgac ccgctcccgc aggcgatggt gctgacggcg
     3841 atcgtcatca cgttcggcgt gaccgcactc ctcttggcgc tggcgtaccg cagctggctg
     3901 ctcaccgacg acgacgaggt cgaggacgac gtctccgacc gggtgatcgc cggtggcggt
     3961 ttcatcgacc gggaggtcac cgatgccgac gccgctctcg ccgacgtgat cgatcccgcc
     4021 ggtgggcacc gggcgacgaa ccggaggcgg cgccgatgac agcgctcctc acgctcccca
     4081 tcctgctgcc gctcatcggt tccgggtgtt cgatgatcgt cgggcgcagt cgggtactgc
     4141 agcggatcgt gtcgttgacc gtgctgtcgg cgatggcggc cgtgtcgatc ggcctgctcg
     4201 tggtcgtcga ccgtgatggc ccgatcgcgg tgcaggccgg tggctgggcc gcgccgatgg
     4261 gcatcacgct cgtcgccgac cgtctgtcgg ccctcatgtt ggccgtggcg tcggtgatgc
     4321 tgttggcggt cctcgtgtac gcgatcggcc agccgggcgc cgaacgcaac cacgtcgggt
     4381 tccagtcggt gtacctcgtg ctgtccgccg gggtcgccgg ggccttcctc accggtgacc
     4441 tgttcaacct cttcgtctcg ttcgagatga tgttggccgc gagctacgtg ctgatcaccc
     4501 tcggggcccg acgcgaccag gtgcgcgccg gcatgaccta cgtggtgatc agtctgatcg
     4561 cgtcgaccct gttcgtgacg gcgctcgcgc tcgtctactc ggcgacgggc accgtcaacc
     4621 tcgccgacct gtcacagcgc atggagcagc tctcgccagg tctgcgcacc gccttctcgg
     4681 tgttgctgct cgtcgtgttc ggcatcaagg cgggtctgtt cccgttgttc ttctggttgc
     4741 ccgacagcta cccgacggcg ccgtccgcgg tgacggcggt attcgccggc ctcctcacga
     4801 aggttggggt ctacgcgatc atccgcaccc agacactcct gttccctccc gaatcgcggc
     4861 cctcgacgct gatcctggtg ttggcgggag cgacgatggt ggtcggcgtg ttgggcgcga
     4921 tcgcgcagga cgacgtgaag cggatcctgt cgttccacat cgtcagccac gtggggttca
     4981 tggtgatggg cctcggcttc ttcaccgtcg ccggtctcgg cggcgccgtc ttctacatcg
     5041 tccaccacat cgtggtgatg acaaccctgt tcctgaccgg cggactcatc gagcacgtgg
     5101 gcgggtccag ccgactgagc cgcgtcggtg ggctggtcac cggcgctccc gtggtcgcag
     5161 tgctcttcct ggtgcccgcc ctgagcctcg ccggcgtgcc gcccttctcg gggttcgtcg
     5221 cgaaactcgg tctcctgcag gccggtgtct ccgcgtccca cggcgtgatc gtcggcgtcg
     5281 ccctcgtcgt cagcctgctc accctgttct cgatgatgaa gatctggtcg gcggtgttct
     5341 ggggcgagaa gccgtcggag cccgctgtgt cactggggac cgcaccgccg ctgatggtcg
     5401 cgcccacgct ggtgctggcg gtcgtgagcc tcgtcatcgc cgtgtcggcc ggtccactgc
     5461 acgatctcgg ggttcgcgcc gcgaccggtc ttctcgatcc gcacgagtat ctcgaagcgg
     5521 tccgccaacc tgaggggctg gcccgttgag ccggctcacg ctcggggtgt gcatcgtcat
     5581 cggctggatg ttcctgtggg gcgacgtcgg tgtcggtccg gtcatcggcg ggacgctcgt
     5641 cgtcgtgacc ctgtatgtcg tgtttcccag ttcgcgggcc tcgttcccgc gtgtcgtggt
     5701 gcacccgttc gccgtggccc ggctgttcgc gtacttcgtt cgtcagctcg tcgtgtcgaa
     5761 tgcgttgctg acccgtgagt tgctgcgccg ccggaactcc atctgcaccc gggtggtcga
     5821 gatcccgatg cgaacctcgg tcccgtcgct cctcaccatg gtcaccaacc tcgcgtcact
     5881 gagtcccggc acgattgtgg tcgacgccga cgagttggct cccgggacac cgccgaccct
     5941 cgtcgtgcac gtgctcatgt tcggcgacct cgacgatccc gaagcggccg tccgttcggt
     6001 ccacgcgctc gaggaacgct gtgtgctcgc gttcgggacg aacgacgcga tccgagagct
     6061 ggagctggcg cgatgaccgt cgtgaccatg ctcgtgttgg ccgcggccgc ggcggcgtac
     6121 ctcgttcgcc tggtccgagg gccggggctg tccgagcgcg tcatcgcgct cgacgggctg
     6181 atcgtggtgg gcatcagcgc cgtcgctgtc cacgcgatgt cgaccggaca gggggcgttc
     6241 ctcccggtgc tcgtggtcat cacgctcgtt gggttcgtcg cgacggccgc ggctgctcgc
     6301 ttcatcgagc gcaccgacgg cgtcgatcag cgaccggggg acgcgcggtg atcgggcaac
     6361 tgctcgcgtt gggcggcgcc gtgctcatct tgttctcggc gctcggcatc gtgcggttcg
     6421 acgacgtgtt cgcccagatg catttcctca ccaaggcgtc gacgttgggg gtcgtgctcg
     6481 ttctcggggg tgccgccctg gtgatggagc gggcgaacga ctggtcgtcg ctcctcttgg
     6541 ccgcgttcct gcagctgttg acgtcgccgg tcgcggccaa cctgatcggc cgttcgacgt
     6601 atctgacgcg ggtccgaccg attcaaggtt caacgaccac gccggtagaa tgagcgctgg
     6661 gatcgacgag ccgggcggac aggtgttgca agcgggtcaa gacatcgaga cggtgccggg
     6721 ctggaccagc ctgatcgccg gcgagccgtg gacggcgtcg gagttgtggc tgtccgcggc
     6781 gtcgatcgac attcggcgcg tcgcgccccg ctggtcgggt gcggcggaac catcggccgg
     6841 gctggagctg tcgggaccac cggagctgac gtcgatcggg ttggccgatg tggtcggcgt
     6901 cgaccacatc ggcgacgatc ccgacggtct ggcgatcctc gagttcgcgc tcgccgccgg
     6961 caacgtggtc cacgcccgcc tgcccgagtc gttcgtggac gaggtcgtcg cggccttgca
     7021 gcggagtcga gccggggtcc cgccgacctt cgagccggca
//
LOCUS       DAOSOA010000153         7045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10728_length_7045_cov_5.692275, whole genome shotgun sequence.
ACCESSION   DAOSOA010000153 DAOSOA010000000
VERSION     DAOSOA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10728_length_7045_cov_5.692275"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PKY13_10240"
     CDS             <1..1023
                     /locus_tag="PKY13_10240"
                     /inference="COORDINATES: protein motif:HMM:NF020119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HOT80357.1"
                     /translation="LIPENTLRWFEFLDFYVAGERTPLPGFLTQFGPALVGDTFGAQL
                     AFPPERLLDGDFATLKAQYEAEPEVNILWDRGARTTNPGAPESTGSTTFAAWPPAGRK
                     AQRWFLQPDGGLGATKPSVASSASRWTTDLSLADKVTLPGKKEGQAFDVAPEFEWDQD
                     AAGDAAVFVSSPLAADTVLLGGGSADLWIRSSGSDADLGLTLSEVRPDGKETYLQSGY
                     LRASLRKEGPKATDLDPDHTGTEADSAPLVPGEWTEARVEILPVGHVLRKGSRIRISI
                     HTPGGDKPRWSWIVDIGHDSAHPSSIVLPVVPGVTGYQKTLPACPSLRGQPCRTFQPY
                     QNTPAP"
     gene            complement(1067..1987)
                     /locus_tag="PKY13_10245"
     CDS             complement(1067..1987)
                     /locus_tag="PKY13_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80358.1"
                     /translation="MQFDLDIRRLAEAQHSLVARPQLSSLGLSPNEVDRLVTSGLWEI
                     AGGRVLRLVGSRRSRAQRLLLPILDQGGNTALAYMACASWWGLRGCSDRPVTLATTGA
                     SRRTTDFAVVRRVRHLPPRWVVPLRGVPTLRPEYLAIHLFADCRFERAEVLVDRLWSM
                     RLLSGPSIELALNDLGRSGRNGIVGARQHLERRPPGYIPTATGLEGRVKRLLEEHGIT
                     LRSQIDSGAEMWTGRVDFRHDTVPFVLEVQSEAFHSALTDVEHDQRRHQRLAADGFTY
                     REVWDTAVWAQPKVVVDTVRDGIAEALGAL"
     gene            complement(2130..3359)
                     /locus_tag="PKY13_10250"
     CDS             complement(2130..3359)
                     /locus_tag="PKY13_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80359.1"
                     /translation="MSIDFTLTPELEDLRLRVRTFVEEVVKPGEAKIGDPDEIERDEY
                     INILFGMRKAAQEAGIWLPHMPKEWGGMGLGHVELAMVQAEAAKAYYGPFVFNCQAPD
                     EGNMHTLLHWATDEQKQKYLKPLCDGFAMSCFAMTEPEVAGSDPTLIRTTAVQDGDEW
                     VINGHKWFISNARRARFAILVCRTEDNPDLPQAANTAFIIDLPSEGWNRVREVETMHG
                     GSGHSEIVIEDLRVPNSNMLGGRGQGHLLGQYRLGPARLAHCMRWVAQAETALDMMVE
                     RSLNRYSHGSMLAEKQGVQWMIADSTMELYQAKLMVLHAAYRIDNGLDFKSEVSMAKH
                     FVANMLGRVIDRSIQVHGALGYSTDTPLAHMYQHARWARFADGADEIHQMRIAQRTIA
                     AFRDEHSTRRATGDLPL"
     gene            complement(3370..5229)
                     /locus_tag="PKY13_10255"
     CDS             complement(3370..5229)
                     /locus_tag="PKY13_10255"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HOT80360.1"
                     /translation="MRLIAVTACPTGVAHTFMAAEALKRQAAAMGHEMSVETQGAQGA
                     ADVLTADEVGAAAAVIVAADVHVDLVRFSGLPMIAVSTADAIRNTAAVIDRALAEVPV
                     EEVPVEEAPVAGSQVDAAPVADASARTAGRRLVAITSCPTGIAHTFMAAEGLQRAAED
                     AGHSIKVETQGSVGAKNVLTAADVAAADAVIIAADTKVDLGRFVGKRIYETGTKEALK
                     HGDDVVAKALASPIVHEGDGGAGSGSSAPAEDGAPAGARAAVKQVYQHLMTGVSYMLP
                     FVVAGGLMIAISFAVNINANDPDLAGSFAGRLFSTGVAALTLFLPVFAGYISFSIADR
                     PGLVPGFIGGYLASQVGAGFLGALLAGFFAGYVTRELANRIKLPVSMAGLKPVLILPL
                     LSTLAVGLVMYYVIGGPISWVQDHLTDWLSGLQGSNAVVLGLILGAMMAADMGGPLNK
                     VAYTFAVGLIGSGVTGPMAAVMAAGMTPPIGLALATKLFPNRWTNDEHGSAAPALVLG
                     LSFITEGAIPFAARDPLRVIPAIVVGSATAGAISMGADSATSVPHGGVFDLFVPGAVE
                     KPLIWLLAVVAGTVVTAGVLFVTKRPIAASPIDLTAVPATVGADTPSSALATV"
     gene            complement(5226..6206)
                     /gene="pfkB"
                     /locus_tag="PKY13_10260"
     CDS             complement(5226..6206)
                     /gene="pfkB"
                     /locus_tag="PKY13_10260"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HOT80361.1"
                     /translation="MTAVVTVTVNPALDQTLWIPGFAAARVNRVEREVVRPGGKGVNV
                     AARLAELGVSVAATGLLGNDNAAPFVALMADRGIEDGFVRQPGATRIGVKIVDPVGDR
                     TTDINFPGEPPDARSLVDLEDRVSALAGGASWIVLAGSLPPGVPTDLYRRLIEVAHER
                     GASVALDTSGEALRRAVEARPDLIKPNEHELEDLVGRRLDRPDDVLDAARELADFGLE
                     FAVVSMGAQGALFCRRDLLVHAAPLQVPVASTVGAGDAMVAGVIAARLRHLPLHAVAA
                     FATACSASAISAVDARLDRGAVEALATTVHVTPKPVVAPAGSRTGIQGES"
     gene            complement(6203..>7045)
                     /locus_tag="PKY13_10265"
     CDS             complement(6203..>7045)
                     /locus_tag="PKY13_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOT80362.1"
                     /translation="DAAVAVEAGAEGIGLMRTEFMFLGRDEPPSEDEQYEVLAEMVRS
                     LNGLPLVVRTLDIGGDKNVPYLDLPHEDNTFLGIRGIRLCFERPDLFAPQLRAIYRAS
                     KLGPVSMMFPMIARIEDLDAAMLHAEIARSEVGAEPIDIGVMIEVPSAALIAPELAKK
                     VQFFSIGTNDLTQYTLAMDRLHPMLAAQADALHPAVLRLIRMVVDAARPEDVWVGVCG
                     GIAGDPEGALILSGLGVDELSMSIPAVGAVKARLRNVSMADARRLAERALACSTADDV
                     RALL"
BASE COUNT         1111 a         2448 c         2444 g         1042 t
ORIGIN      
        1 ctcatccccg agaacaccct gcggtggttc gagttcctcg acttctacgt cgccggcgag
       61 cggaccccgc tgcccggctt cctgacgcag ttcgggcccg cactggtcgg cgacacgttc
      121 ggcgcccagc tcgccttccc gccggagcga ttgctcgacg gagacttcgc caccctgaag
      181 gcgcagtacg aggccgaacc ggaggtcaac atcctctggg accggggtgc ccggaccacc
      241 aaccccggtg cgcccgagag caccggatcg accacgtttg ccgcctggcc gccagccggc
      301 cgcaaggcac agcgctggtt cctccagccc gacggcggcc tcggggccac gaagcccagc
      361 gtggcgtcat cggcatcgcg gtggacgacc gacctctcgc tggccgacaa ggtcacgctc
      421 ccgggcaaga aggagggcca ggcgttcgac gtcgcccccg agttcgagtg ggaccaggac
      481 gccgccggcg acgccgcggt gttcgtctcg tcgccactgg cggccgacac ggtgctgctg
      541 ggtggcggca gcgccgacct gtggatccgg tcgagcggct ccgacgccga cctcggcctg
      601 accctgtcgg aggtgcgtcc cgatggcaag gagacctacc tccagtcggg ctacctgcga
      661 gcgtcgctcc gcaaggaggg cccgaaggcc accgatctcg accccgacca caccggtacc
      721 gaggcagatt cggcgccgct cgtgcccggc gagtggaccg aggcccgcgt cgagatcctt
      781 ccggtcggac acgtgctccg gaaggggtca cggatccgca tcagcatcca cacacccggc
      841 ggcgacaagc cgcgttggtc gtggatcgtc gacatcggtc acgactcggc gcacccgtcg
      901 agcatcgtgc tccccgtcgt gcccggcgtg accggctacc agaagacgct gccggcatgc
      961 ccctcgctcc ggggccagcc ctgccgcacc ttccagccgt accagaacac ccccgccccc
     1021 taggggtttt gtgctgcgag gagcgacgct gacgtcgctc ctcgcactac aaagccccga
     1081 gggcttcggc gattccgtcg cgcaccgtgt cgacgacgac cttcggctgg gcccagaccg
     1141 ccgtgtccca tacctcgcgg tacgtgaacc cgtccgcagc cagccgctgg tggcgacgtt
     1201 ggtcgtgctc gacgtcggtc agggcggaat ggaacgcttc gctctgcacc tcgagaacga
     1261 acggcacggt gtcgtgacgg aagtcgaccc gccccgtcca catctcggca ccactgtcga
     1321 tctgcgatcg caacgtgatg ccgtgctcct cgaggagccg cttgacccga ccctcgaggc
     1381 cggtcgccgt cgggatgtag ccggggggac gtcgctccag atgctgcctc gctccgacga
     1441 tgccgttacg gcccgaccgg cccagatcgt tgagcgcgag ctcgatggat ggtcctgaca
     1501 gcaggcgcat cgaccacagc cggtcgacga ggacctccgc ccgctcgaag cgacagtccg
     1561 cgaacagatg gatggcgagg tactccggtc gcagtgtcgg gaccccgcga agcggcacga
     1621 cccaacgagg cgggaggtga cggacgcgcc gtaccactgc gaagtccgtg gtacgccgcg
     1681 acgcgccggt ggtcgcgagg gtgaccggtc ggtcgctgca gccgcgaagg ccccaccacg
     1741 acgcgcacgc catgtaggcg agggccgtgt tcccaccttg gtcgaggatc ggcaggagga
     1801 ggcgctgcgc ccgcgagcgg cgtgacccga ccagtcggag cacgcgaccc ccggcgatct
     1861 cccacagacc gctcgtgacc aggcggtcca cctcgttggg ggacaatccc aaggagctga
     1921 gctgcggccg agccactagg gagtgctgcg cctctgccag gcgacgaatg tccaggtcga
     1981 actgcatgca gcgaacgctg cctgcagtcg ctcacgactg ccatgggggc catcccccat
     2041 cgggtttcgt agtgcgagga gcgacgtcag cgtcgctcct cgcagcacaa aaccccgagg
     2101 gtgcggaggg ccggtcgggg ctgtcggggc tacaggggga ggtcgccggt ggcgcgtcgg
     2161 gtggagtgct cgtcgcggaa tgcggcgatg gtgcgctgag cgatccgcat ctgatggatc
     2221 tcatcagcac cgtcggcgaa ccgggcccag cgggcgtgct ggtacatgtg ggcgaggggc
     2281 gtgtcggtcg agtacccgag cgcgccgtgc acttggatcg accgatcgat gacccggccg
     2341 agcatgttgg ccacgaagtg cttggccatc gacacctcgg acttgaagtc gaggccgttg
     2401 tcgatgcgat aggcggcgtg gagcaccatg agctttgcct ggtacagctc catcgtggag
     2461 tccgcgatca tccactggac accctgcttc tcggccagca tcgagccgtg gctgtagcgg
     2521 ttgagcgagc gctcgaccat catgtcgagc gccgtctcgg cctgagccac ccaacgcatg
     2581 cagtgagcca gacgggcggg cccgagccga tactggccca ggagatggcc ctgcccccgg
     2641 ccgccgagca tgttcgagtt cggcacccgg agatcctcga tgacgatctc gctgtgaccc
     2701 gaaccaccgt gcatcgtctc gacctcacgc acccggttcc acccctctga gggcaggtcg
     2761 atgatgaagg cggtgttggc ggcctgcggc aggtcagggt tgtcctcggt gcgacacacg
     2821 aggatcgcga acctggcccg ccgggcattg gagatgaacc acttgtggcc gttgatgacc
     2881 cactcgtcgc cgtcctgcac agcggtcgtg cggatgaggg tcgggtccga gcccgccact
     2941 tcgggctccg tcatggcgaa gcaactcatc gcgaatccgt cgcacagcgg cttgaggtac
     3001 ttctgcttct gctcgtcggt cgcccagtgg agcagcgtgt gcatgttgcc ctcgtcgggc
     3061 gcctggcagt tgaacacgaa cgggccgtag tacgccttgg cggcctcggc ctgcaccatg
     3121 gccagctcga cgtggccgag gcccatgcca ccccactcct tcggcatgtg cggcaaccag
     3181 atgcccgcct cctgcgccgc cttgcgcatg ccgaacagga tgttgatgta ctcgtcgcgc
     3241 tcgatctcgt cggggtcgcc gatcttcgcc tcgcccggct tgaccacctc ctcgacgaag
     3301 gtgcggaccc gtaggcggag gtcctcgagc tcgggcgtga gggtgaagtc gatcgacatg
     3361 cgccggagac taaaccgtgg cgagcgccga tgacggggtg tccgctccga ccgtcgctgg
     3421 cacggccgtg aggtcgatcg gggaggcggc gatcggccgc ttggtgacga acagcacccc
     3481 ggcggtcacc accgttcccg cgaccacggc gaggagccag atcaggggct tctcgaccgc
     3541 acccggcacg aacaggtcga agaccccgcc gtgcggcacc gatgtcgccg aatccgcgcc
     3601 catcgagatc gccccggctg tcgcggagcc gacgacgatc gccgggatca cccggagcgg
     3661 atcgcgggcg gcaaacggga tcgccccttc ggtgatgaac gacaagccca gcacgagcgc
     3721 cggtgcggcg gagccgtgct cgtcgttcgt ccaccggttc gggaacagct tggtggcgag
     3781 cgccaggccg atcggcgggg tcatgccggc agccatgacg gccgccatcg gtccggtcac
     3841 cccgctgccg atcagcccga ccgcgaaggt gtacgccacc ttgttgaggg gcccacccat
     3901 gtcggcggcc atcatcgctc cgaggatcag accgagcacc acggcgttgc tcccctgcaa
     3961 cccgctcaac cagtcggtca ggtgatcctg cacccaggag atcggcccgc cgatcacgta
     4021 gtacatgacg agaccgacgg cgagcgtcga caggagcggc aggatcagga cgggtttgag
     4081 gccggccatc gacaccggga gcttgatgcg gttcgccagc tctcgggtca cgtagccggc
     4141 gaagaacccg gcgaggagcg ccccgaggaa gccggcaccg acctgtgacg cgaggtagcc
     4201 gccgatgaat cccggcacga gcccgggccg gtcggcgatc gagaagctga tgtaaccggc
     4261 gaagaccggg aggaacagcg tgagggcggc gacgcccgtc gagaacaggc gaccggcgaa
     4321 actcccggcc aggtcggggt cgttggcgtt gatgttgacg gcgaacgaga tggcgatcat
     4381 caggccgccg gcgacgacga acgggagcat gtaggacacc ccggtcatca ggtgctggta
     4441 cacctgcttg acggcggccc gggcgcctgc gggtgccccg tcctcggccg gcgcggacga
     4501 cccacttcct gccccgccgt cgccctcatg gacgatgggc gacgccaggg ccttcgcgac
     4561 gacgtcgtcg ccgtgcttga gcgcctcctt ggtgccggtc tcgtagatcc gcttcccgac
     4621 gaatcgaccg aggtcgacct tggtgtcggc agcgatgatc acggcgtcgg ccgccgcgac
     4681 gtctgccgcc gtgaggacgt tcttcgcccc gaccgagccc tgggtctcga ccttgatcga
     4741 gtgcccagca tcctccgccg cccgctggag cccttcggcc gccatgaacg tgtgggcgat
     4801 gcccgtcggg cacgacgtga tcgccacaag gcgtcgtcca gccgttcgcg cggatgcatc
     4861 ggcaaccggc gctgcgtcaa cctggctccc agcgaccggc gcctcctcga ccgggacctc
     4921 ctcgaccgga acctcggcga gggctcgatc gatcaccgcg gcggtgttgc ggatggcatc
     4981 ggcggtcgac acggcgatca tcggaaggcc cgagaagcgc acgaggtcga cgtggacgtc
     5041 agccgccacg atcacggcag cggccgcccc gacctcgtcg gccgtgagga cgtcggcggc
     5101 gccctgggcc ccctgcgtct cgacggacat ctcgtgtccc atcgccgcgg cttggcgctt
     5161 gagcgcctcg gccgccatga aggtgtgcgc gacccccgtc gggcacgctg tgactgcgat
     5221 caacctcatg attccccctg gattcctgtt cgacttccgg ctggtgcaac aactggcttg
     5281 ggtgtgacgt ggacagtggt ggcgagcgcc tcgacggcac cgcggtcgag acgggcgtcg
     5341 accgccgaga tggccgacgc agagcacgcc gtggcgaagg cggcgacggc gtgcagcggg
     5401 agatgcctga ggcgagcggc gatgaccccg gcgaccatcg cgtcgccggc gcccaccgtg
     5461 ctcgcgacgg gaacctgcag tggcgcagcg tgcaccagga ggtcccgtcg gcagaacagc
     5521 gcgccctggg cgcccatcga gaccacagca aattcgagac cgaagtcggc gagctcgcgg
     5581 gcggcgtcca ggacgtcgtc ggggcggtcg agtcgtcgtc ccaccaggtc ctcgagttcg
     5641 tgctcgttgg gcttgatgag gtcgggtcgc gcttcgacgg ctcggcggag ggcctcgccg
     5701 ctcgtgtcga gtgcgaccga cgcgccccgc tcgtgcgcca cctcgatgag ccgccgatag
     5761 aggtcggtcg gaacaccggg gggcaggctg ccggcgagca cgatccacga cgcgccgccc
     5821 gccagcgcgc tcacgcggtc ctcgaggtcg acgagggagc gggcgtcggg cggctctccg
     5881 gggaagttga tgtcggtcgt tcgatcgccg acgggatcga cgatcttcac gccgatgcgc
     5941 gtggcgcccg gctgccggac gaatccgtcc tcgatgccac gatcggccat cagggccacg
     6001 aacggcgcag cgttgtcgtt gccgagcagg ccggtggcgg cgacggagac accgagctcg
     6061 gcgaggcggg cggcgacgtt gacacccttg ccccccggcc tcacgacctc gcgctcgacc
     6121 cggttcaccc gggccgcagc gaaaccggga atccacaggg tctggtcgag cgccggattg
     6181 acggtgaccg tgacgacggc ggtcacagga gcgcccggac gtcgtcggcg gtcgaacacg
     6241 cgagcgcccg ctcggcgagc cggcgagcgt cggccatcga aacgttgcgg agccgcgcct
     6301 tgactgcgcc gacggcgggg atgctcatgg acagctcgtc gaccccgagt ccgctgagga
     6361 tgagggcacc ctcggggtca ccggcgatcc cgccgcacac gccgacccac acgtcctcgg
     6421 ggcgggcggc gtccacgacc atgcggatca gtcgcaggac cgccgggtgc agcgcgtccg
     6481 cctgcgcggc gagcatcgga tggagccggt ccatcgccag ggtgtactgg gtgaggtcgt
     6541 tcgtgccgat cgagaagaac tgcaccttct tggcgagctc cggcgcgatg agtgcggcgg
     6601 acggcacctc gatcatcacc ccgatgtcga tcggctcggc gccgacctcg ctccgggcga
     6661 tctcggcgtg cagcatcgcc gcgtcgaggt cctcgatccg ggcgatcatc gggaacatca
     6721 tcgagaccgg cccgagcttc gaggcccggt agatcgccct gagttgcggg gcgaacaggt
     6781 cgggccgctc gaagcacagc cggatacccc ggatgccgag gaacgtgttg tcctcgtgcg
     6841 gaagatcgag gtacgggacg ttcttgtcgc cgccgatgtc gagcgtacga accacgagcg
     6901 gcagcccgtt gagcgagcgc accatctccg ccaggacctc gtactgctcg tcttccgacg
     6961 gtggttcgtc acgcccgagg aacatgaact cggtgcgcat caggccgatc ccctcggcgc
     7021 cggcctcgac cgccacggcg gcgtc
//
LOCUS       DAOSOA010000154         7033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10750_length_7033_cov_5.375466, whole genome shotgun sequence.
ACCESSION   DAOSOA010000154 DAOSOA010000000
VERSION     DAOSOA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10750_length_7033_cov_5.375466"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1990)
                     /locus_tag="PKY13_10270"
     CDS             complement(188..1990)
                     /locus_tag="PKY13_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT80363.1"
                     /translation="MNPFIGRKAFVGLASVVATAVLVVDLVVGGAASAQTASTPGDAA
                     SGVVAITVTADGALDPAAVAAATGGTGRITATIDAIGATTVEVPAGRALDAVRRLGAV
                     PEVDTAQADQQVRAFERVPTDPYWPQQEPAMRGIGAPAAWGVSTGAPSVVIAVVDSGV
                     NPVGDLAGRVLPGYDFVNGDSDASDDADHGTESALVAAAAGDAGGGTAGMCWSCQVLP
                     VKVLDSDGVGSMFDVAAGIVWAADHGADVINLSLGGRFSDPAVTDALNYAYARDVVVV
                     ASAGNDGKSEQSYPAAEAGVISVAGIVNGQRNLHPLSRRGPTWVDVAAPWCNWVVMGS
                     PDYFCGTSSSAPLVTGTAALLRTIRPAADRQMIATALLTTADHAPTTGLVGYGILDAG
                     RAAAAVSTVAPGPTPNPPDVTPPTATMTAPGGYQIGSTVVQVVSDDDQGIVMVEVRLD
                     GTLVAALANPARVQDLRFDTGSFGDGPHVVEAVVTDVGGHRVLAGSATMVTDNANPLG
                     LLVSPSAGAKVSGSYVARVFVTDPNGIMGTFFIANDQVVGGFLGAGFGQVTVPVTRNG
                     PIRVVALTVDNAGHISGTNTAVVTGSVPRRKARR"
     gene            complement(1987..2622)
                     /gene="pgsA"
                     /locus_tag="PKY13_10275"
     CDS             complement(1987..2622)
                     /gene="pgsA"
                     /locus_tag="PKY13_10275"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOT80364.1"
                     /translation="MIHRPDADEQGLGDLAVDRIVTLPNIITTVRLACVPLFLWLLFG
                     RDAPGWAGFLLGTLGATDWVDGYVARRFDQTSNFGKMYDPTVDRFMMVVGIVAIIVDG
                     AAPLWFAWLILVREVLVSLWVVAITAMGAKRMDVTWWGKCGTFANMGAFPAFLIASQP
                     GMSDWWTTTWKVVAYVCLVPGVIFSLLAAGQYVVKGREALAEGRAEREAAA"
     gene            2696..>3123
                     /locus_tag="PKY13_10280"
     CDS             2696..>3123
                     /locus_tag="PKY13_10280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOT80365.1"
                     /translation="MTDETPEPTLFTRIIRGDLPGRFVWRDDEIVAFLTIAPHQPGHT
                     LVVPVAPVDRWTDLADEVWIRLNVVALEIGRAMETAFDCVRVGTVIAGLEVPHCHVHL
                     IPIQSEADLGFGNADHHASPESLDAAAERLRAELRARGNGA"
     assembly_gap    3124..3223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3228..3959)
                     /locus_tag="PKY13_10285"
     CDS             complement(3228..3959)
                     /locus_tag="PKY13_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOT80366.1"
                     /translation="MCGRFVSASPPEEIAEYFAAMMMGDPLEPNFNVAPTLDVYVVYE
                     DGSERKLDQFHWGLVPGFAKDIRIGNRMINARAETLATNNSFKHSFARRRCIVPADGF
                     YEWQAVAGQKRKQPYFIHRKDDEPLAFAGLWAEWRGQVAGEQVVVRSTTIITTEANET
                     MAPVHDRMPVILPRRAWDDWLDPSNLQVERLQPLLVPAPASLLTMHPVSTDVSNVRNV
                     GRHLAEPIPAEQVLGAGDPEQQSLL"
     gene            4031..5224
                     /locus_tag="PKY13_10290"
     CDS             4031..5224
                     /locus_tag="PKY13_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT80367.1"
                     /translation="MTDPHSTAGNRRFGERRRAPQLSVITPTFNEVDNVVPLLDKLEA
                     ALGDLSHEILVVDDDSPDRTWEVAEEYAATHPSVRVLRRFSDHGLSSAVMAGMEASTG
                     KVIAVIDADMQHDERVLPDMVAVLESGDADLVVGSRAADGGSYGEWSAFRRLVSWVAA
                     MIARVFLRVSTTDPMSGFFAISRDAYRTAAPAVNAQGFKILLEFIGRNPGLRVAEVGY
                     EFRNRVAGETKLSPSIIRSYLLAVFELRFGRQVKGQFFLYCLVGASGVMINLAVFSLF
                     ELLDAGSIDVGFARPLRWSLIAGIAASVVTNFVLNNYFTFWERRYRRHHLVQGFALFA
                     LVSGLGVIVHVAVFQFLQENGWGNDVFGEGPTRLIHDGVGFVVALVSNYFLNVNYIWR
                     RRPEV"
     gene            complement(5246..6688)
                     /locus_tag="PKY13_10295"
     CDS             complement(5246..6688)
                     /locus_tag="PKY13_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80368.1"
                     /translation="MTVVVVVAGVVGWAASARLTGTPDSLDYLVAARSWLAEGRPLDT
                     AGRPFTQFAPGYPAALAALTWFGAELLTAARVVAAVATAVTAFAAGRWARRITGSSRA
                     GVVAAVAAVSIWPVWTAAHAWSESLLIALAVSAGAVLSAGVARRSVPLVGAAGILVGC
                     AAATRYAGLVFVLLALPLSVQWGGTLRQRWLAASVFVLPVLVIGGAPVVHNLTVDRSQ
                     PLGPRAPADASTLDVLMQAVTGVVKLAAPPDAPALVGVVSFGIVAVVVTLAARSLSAP
                     QRSDLRRSAALPVGFAGLYFVATVAGAVGSRLDRLDDGRLLAPCGPLLAVAAVALVSP
                     AVGALSERTSGVRPALRWTRLGLLAAAGAAAINVVVSNGLARRPQGLLSPAVVRSDVL
                     QEAAAADAPLVVSNSPGVVAWALDRPVVTPPLRGAGRAADGWPAGTLMVWMDVGLADV
                     ADLDDLTGACDVDERADLAGGKLVVVNRCA"
     gene            complement(6786..>7033)
                     /locus_tag="PKY13_10300"
     CDS             complement(6786..>7033)
                     /locus_tag="PKY13_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOT80369.1"
                     /translation="EDVDAIGLSMLSGAHLALVPPVVELLREQGLTIPVVVGGIIPER
                     DVPVLEAAGVAAILAPGASSAEVVATIESVIAANSGV"
BASE COUNT         1078 a         2470 c         2442 g          943 t
ORIGIN      
        1 ctcccggtgg accgcgatcg accgcggcgc ctcgatgccg agccggaccg tgccaccctc
       61 gagccggacc cactcgtggt cggaggagta gcgcagatcg tcgggaaagt tcatggccac
      121 ggactctagt ggatcgccgg acccgccagc aggggtccga cggtggcgac ggcgaccggc
      181 gcgggcgtca ccgccgcgcc ttgcggcggg gcaccgaccc ggtgaccacg gcggtgttcg
      241 tgcccgagat gtggccggcg ttgtcgacgg tgagtgcgac cacgcggatg gggccgttgc
      301 gggtgacggg gaccgtgacc tgcccgaatc cggcgccgag gaacccgccg acgacctggt
      361 cgttggcgat gaagaaggtg cccatgatgc cgttggggtc ggtcacgaag acccgggcga
      421 cgtaggagcc cgacaccttg gcaccggcgg acgggctgac gaggaggccg agggggttgg
      481 cgttgtcggt gaccatggtc gccgagccgg ccagcacgcg gtggccgccg acgtcggtga
      541 cgacggcctc gacgacgtgg ggcccgtcac cgaaggaacc cgtgtcgaac cggaggtcct
      601 gcacccgcgc cgggttggcg agggcggcga cgagggtgcc gtcgaggcgc acctcgacca
      661 tcacgatgcc ctggtcgtcg tcgctcacga cctgcacaac ggtggatccg atctggtagc
      721 caccgggagc ggtcatcgtt gcggtcggcg gcgtcacgtc gggcgggttc ggcgtcgggc
      781 ccggggcgac cgtcgacacg gccgcagcgg cccggccggc gtcgaggatg ccgtagccga
      841 cgaggccggt cgtcggcgcg tggtcggcgg tcgtgagcag cgccgtcgcg atcatctggc
      901 gatcggcggc gggtcggatc gtccgcagca gtgctgccgt gccggtcacg agcggcgccg
      961 aggacgacgt gccgcagaag tagtcgggcg aacccatcac gacccagttg caccacggtg
     1021 ccgccacgtc gacccaggtc ggacctcgcc ggctcaacgg gtggaggttg cgttgaccgt
     1081 tgacgatgcc ggcgaccgag atgaccccgg cctcggcagc cgggtagctc tgctcgctct
     1141 taccgtcgtt gcccgccgag gccacgacga ccacgtcgcg ggcgtaggcg tagttgaggg
     1201 cgtcggtcac ggccgggtcg ctgaaccgac cgccgaggct gaggttgatg acgtcggcac
     1261 cgtggtcggc cgcccacacg atgccggcgg cgacgtcgaa catcgagccg acaccgtcgc
     1321 tgtcgagcac cttcaccggc agcacctggc agctccagca catgccggcg gtcccgccgc
     1381 cggcgtcacc ggccgcggcg gcgacgagcg ccgactcggt gccgtgatcg gcgtcgtcgg
     1441 aggcgtcgga atcgccgttg acgaagtcgt agcccggaag gacccggccc gcgaggtcgc
     1501 cgaccgggtt gacccccgag tcgacgacgg cgatgacgac cgacggcgcg cccgtcgaga
     1561 caccccacgc cgccggtgcg ccgatgccac gcatcgcggg ctcctgttgt ggccagtacg
     1621 gatcggtcgg cacccgttcg aaggcgcgga cctgttggtc ggcctgggcg gtgtcgacct
     1681 cgggcacagc gccgagacgg cggactgcgt cgagggcccg gccggccggc acctcgacgg
     1741 tcgtggcgcc gatcgcgtcg atcgtggcgg tgatgcgacc ggtgccgccc gtcgctgcgg
     1801 cgacggccgc cgggtcgagc gcgccgtccg cggtgaccgt gatggcgacc acgcccgacg
     1861 cggcatcacc gggcgtcgat gccgtctggg ccgaggcagc gcccccgacc acaagatcga
     1921 cgacgagcac cgcggtcgcc acgaccgacg cgaggccaac gaaggccttc cgcccgatga
     1981 acggattcac gctgcggcct cccgctcggc acggccctcg gccagtgcct cacgcccctt
     2041 gacgacgtac tgcccggcgg cgaggagcga gaagatcaca cccggaacga ggcacacgta
     2101 ggcgacgacc ttccacgtgg tcgtccacca gtcggacatg ccgggctggc tggcgatcag
     2161 gaacgccggg aacgcaccca tgttcgcgaa ggtgccgcac ttaccccacc acgtgacgtc
     2221 catgcgcttg gcgcccatcg ccgtgatggc cacgacccac agcgatacca gcacctcacg
     2281 aaccaggatg agccacgcga accacaaggg cgccgcgccg tcgacgatga tcgccacgat
     2341 gccgaccacc atcatgaacc ggtcgacggt cgggtcgtac atcttgccga agttggacgt
     2401 ctggtcgaag cggcgggcga cgtagccgtc gacccagtcg gttgcgccca acgtgccgag
     2461 caggaacccg gcccaccccg gtgcgtcgcg cccgaacaac agccacagga acagtgggac
     2521 gcaggccagg cgaacggtcg tgatgatgtt gggcagcgtc acgatccggt cgacggccag
     2581 gtcgcccagg ccctgctcgt cggcgtcggg gcgatggatc aagtgatgat cgtggctgct
     2641 cacagacgaa gagcctacgc cccaccaccg acaacctccc cgttacgatt cgaccatgac
     2701 cgacgagacc cccgagccca cgctgttcac ccgcatcatc cggggcgatc tccccgggcg
     2761 tttcgtgtgg cgagacgacg agatcgtggc gttcctgacc atcgcaccgc accagccggg
     2821 gcacaccctc gtggtgccgg tcgcgccggt cgaccgctgg accgacctcg ccgacgaggt
     2881 ctggatccgg ctcaacgtcg tggcgctcga gatcggtcgg gccatggaga cggcgttcga
     2941 ctgcgtgcgc gtcggcaccg tcatcgccgg cctcgaggtg ccgcactgcc acgtgcacct
     3001 catcccgatc cagtccgagg ccgacctcgg cttcggcaac gccgaccacc acgcgtcacc
     3061 cgagtcgctc gatgccgccg ccgaacggct gcgggccgag ctgcgagctc gcggcaacgg
     3121 cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgcta cagcagcgac
     3241 tgttgctcgg ggtcgccggc gccgagaacc tgctccgcgg ggatcggttc ggccaggtgc
     3301 cggccgacat tgcggacgtt gctgacgtcg gtcgacaccg gatgcatcgt gagcagcgac
     3361 gccggtgcgg gcacgagcaa cggctgcaac cgctcgacct gcaggttgga cggatcgagc
     3421 cagtcgtccc acgcccgccg cgggaggatc accggcatgc ggtcgtgcac gggcgccatc
     3481 gtctcgttgg cctcggtcgt gatgatggtg gtcgaccgca ccacgacctg ctcccctgcc
     3541 acctggccac gccactccgc ccacagaccg gcgaacgcca gcggctcgtc gtccttccgg
     3601 tggatgaagt acggctgctt gcgcttctga ccggcaacgg cctgccattc gtagaagccg
     3661 tccgccggga cgatgcaccg ccgccgggcg aaggagtgct tgaacgagtt gttggtcgcc
     3721 agcgtctccg cccgggcgtt gatcatgcgg ttgccgatcc tgatgtcctt cgcgaacccc
     3781 gggacgagcc cccagtggaa ctggtcgagc ttccgctcac tgccgtcctc gtagaccacg
     3841 tacacgtcga gggtcggtgc gacgttgaag ttgggttcga gcggatcgcc catcatcatc
     3901 gccgcgaagt actcggcgat ctcctccggt ggcgatgccg acacgaacct tccgcacatg
     3961 tctccgaccg tagttcgggc caccgacgct caccggagcg cgccgcctcc gcaactaggt
     4021 tcttcggatc gtgaccgatc cccactcaac cgccggcaac cgccggttcg gtgaacgccg
     4081 ccgggcaccc cagttgtcgg tgatcacccc gaccttcaac gaggtcgaca acgtggtgcc
     4141 cctgctcgac aagctcgagg ccgcactggg cgacctgagc cacgagatcc tcgtggtgga
     4201 cgacgactcc cccgaccgca cctgggaggt cgccgaggag tacgccgcga cccaccccag
     4261 cgtgcgggtg ttgcgccgct tcagcgacca cggcctcagc tccgccgtga tggccggcat
     4321 ggaggcgagc accggcaagg tgatcgccgt catcgacgcc gacatgcagc acgacgagcg
     4381 ggtgctgccc gacatggtgg cggtgctcga gtcgggcgat gccgatctcg tcgtcggctc
     4441 ccgcgccgcc gacggtggga gttacgggga atggtcggcc ttccgccgcc tcgtgtcctg
     4501 ggtcgccgcc atgatcgccc gggtcttcct gcgggtctcg acgaccgacc ccatgtcggg
     4561 gttcttcgcg atctcccgcg atgcctaccg aacagccgca ccggcggtga atgcccaggg
     4621 gttcaagatc ctgctcgagt tcatcggccg caaccccggc ctccgcgtcg ccgaggtcgg
     4681 ctacgagttc cgcaatcgtg tcgccggcga gacgaaactg tcgccgtcga tcatccggtc
     4741 ctacctgctc gcggtgttcg agttgcgctt cggacgtcag gtgaagggcc agttcttcct
     4801 gtactgcctc gtcggggcct ccggcgtcat gatcaacctc gcggtgttct cgctgttcga
     4861 actgctcgat gccgggtcca tcgacgtcgg gttcgcccgc cccctgcgct ggtcgctcat
     4921 cgccggcatc gccgcgtcgg tcgtcacgaa cttcgtgctc aacaactact tcacgttctg
     4981 ggagcgccgg taccggcgcc accacctcgt gcagggcttc gcgttgttcg cgctggtatc
     5041 gggcctcggc gtgatcgtgc acgtggccgt gttccagttc ctgcaggaga acggctgggg
     5101 caacgacgtg ttcggtgagg gtccgacccg gctgatccac gacggcgtcg ggttcgtcgt
     5161 ggcgctcgtg agcaactact tcctcaacgt caactacatc tggcgacgcc gacccgaggt
     5221 gtgagcctcg gagctctggg accggtcatg cgcaccggtt gacgacgacg agcttcccgc
     5281 cggcgagatc ggcgcgctcg tcgacgtcgc aggcgcccgt gaggtcgtcg aggtcggcga
     5341 cgtcggcgag cccgacgtcc atccagacca tcagggtgcc cgccggccaa ccgtcggcgg
     5401 cgcgtccggc gccacggagc ggcggcgtga cgaccggtcg atcgagcgcc catgcgacca
     5461 cacccgggct gttgctcacg accaatggcg cgtcggcggc agccgcctcc tgcagcacgt
     5521 cggaacgcac gaccgccggc gacaggagtc cctggggccg gcgggcgaga ccgttgctca
     5581 ccacgacgtt gatcgccgcc gctcccgccg cagcgaggag ccccagtcgg gtccatcgaa
     5641 gggccggccg aacgcccgag gttcgctcgc tgagcgcacc gacggcgggc gagaccagcg
     5701 cgaccgcagc cacggcgagg agtggtccgc acggcgccag gagccggccg tcgtcgagcc
     5761 gatcgaggcg cgagcccacc gcaccggcga cggtcgcgac gaagtagagc ccggcgaatc
     5821 ccacgggaag ggccgccgac cggcggaggt cggaacgctg cggagccgac agcgaccgtg
     5881 cggccagggt cacgaccacc gccacgatgc cgaacgacac gacgccgacg agcgccgggg
     5941 cgtcgggcgg ggccgccagc ttcaccaccc cggtgaccgc ctgcatcagc acgtcgaggg
     6001 tcgacgcgtc ggcgggggcc cgcgggccga ggggctgtga ccggtcgacc gtcaggttgt
     6061 ggacgaccgg cgccccaccg atcacgagga ctggcaggac gaacacgctc gcggcgagcc
     6121 accgctgccg cagcgtccca ccccactgca cggacagcgg gagcgccaac agcacgaaca
     6181 cgagaccggc gtaccgcgtc gccgcggcgc accccaccag gattccggca gcgccgacga
     6241 gcggcacgga gcggcgcgcc acgccggcgg agagcacggc gccggcagag acggccaacg
     6301 cgatcaggag cgattccgac cacgcatgag cggccgtcca caccggccag atcgacacgg
     6361 cggcaaccgc ggcgacgacg ccggcgcggg acgaaccggt gatccggcgt gcccaccggc
     6421 ccgcagcgaa cgcggtcacc gcggtcgcca ccgccgccac gacccgcgcc gcggtgagga
     6481 gttccgcccc gaaccacgtg agggctgcca gcgcggcggg gtagcccggg gcgaactggg
     6541 tgaacggccg cccggcggtg tcgagcggtc gaccctccgc cagccacgag cgggccgcga
     6601 ccaggtagtc gagtgaatcg ggggtgccgg tcaggcgagc gctcgccgcc cagcccacga
     6661 cgccggcgac gaccaccacg acggtcatcg ggagcgctga acgcgccggg aggcgggtcg
     6721 ggacaacgat ccacccaatg taacgttatt ctccggcgtc gctccgtcgg ggtgcggatt
     6781 cggggtcaga cgccggagtt ggcggcgatg acggactcga tcgtcgcgac gacctcggcc
     6841 gacgacgcac ccggtgccag gatcgcggcg accccggcgg cctcgagcac cggcacgtcg
     6901 cgttcgggga tgatgccgcc gacgacgacg gggatggtca ggccctgctc gcgcagcagc
     6961 tccacgaccg gagggacgag cgcgaggtgg gcgcccgaga gcatcgagag gccgatggcg
     7021 tcgacatcct cgt
//
LOCUS       DAOSOA010000155         7013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10791_length_7013_cov_5.831992, whole genome shotgun sequence.
ACCESSION   DAOSOA010000155 DAOSOA010000000
VERSION     DAOSOA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10791_length_7013_cov_5.831992"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PKY13_10305"
     CDS             complement(<1..953)
                     /locus_tag="PKY13_10305"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOT80370.1"
                     /translation="MIRVDVDLGERSYPVLVGPEVRHELAAVLDPAVRRVAIVTEEGL
                     DIPVDPGRPHEVFEIRGGETNKTLATVEDLCRQFARWELTRADAVVGVGGGIVTDIAG
                     FVASVYHRGLPVVQVATTLLAQIDAAVGGKTGVNLPEGKNLVGAYWQPSAVLCDTEVL
                     ATLPPDEWRCGLGELAKYHWLGGGDLDALELDERVAACVRIKADVVASDEREGGRRAI
                     LNYGHTLAHAIETASDHALRHGEAVAIGLLYAAEVAYRLGRIDAAAVAEHRRVIGAYE
                     LRSTVPDGLSDDELIGLFARDKKATREVTFVLDGPNGVEPVT"
     gene            complement(950..1516)
                     /locus_tag="PKY13_10310"
     CDS             complement(950..1516)
                     /locus_tag="PKY13_10310"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT80371.1"
                     /translation="MTPSSPPPLHLAFIGMAGVGKSAISRRIAQMLGVDVVDIDAAVV
                     DAAGRPIPEIFATDGEARFRQMETDALRAALERPDPTVIATGGGIVESHTNRDLLARR
                     ARVVLLHTTDAVLVERLRNSSNRRPLLEGDLEANLAALRQRRDHLYREVADLAVEVGF
                     FDLAGTSGVVLAAIAERWPDTESQESPR"
     gene            complement(1526..2203)
                     /locus_tag="PKY13_10315"
     CDS             complement(1526..2203)
                     /locus_tag="PKY13_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80372.1"
                     /translation="MLYLVFNEGYSGDVDLAAEAIRLTRQLSRLADDPEVHGLLALML
                     LHHARRTSRTGPDGRLVPLAEQDRSQWDTELIAEGVDVLQAALARDRLGEYQAQAAIA
                     ALHADAPRVEETDWVQIVEWYDELLRYTDTPVVRLNRAVAVGEADGAPAGLVALSDVD
                     ESVPRHAAVAAHLHERNGDIELAAELYAAAAHDAPTLAERAHQTLQAARCNQQLRNGS
                     GRRNPDR"
     gene            complement(2711..3118)
                     /locus_tag="PKY13_10320"
     CDS             complement(2711..3118)
                     /locus_tag="PKY13_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOT80373.1"
                     /translation="MAKYLLLKHYRGAPAPANNIPMERWTPDEIDAHIQFMGDFAARL
                     EASGEFVDGQALAPEGMWVRSDGAGRPPVTDGPFAETKDLIAGWMIIDVDSEARAIEL
                     AGELSSAPGANGEPIHEWLEVRPFLTAPPTITD"
     gene            complement(3233..>3762)
                     /locus_tag="PKY13_10325"
     CDS             complement(3233..>3762)
                     /locus_tag="PKY13_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80374.1"
                     /translation="ETVPVETGPFEAASGTFDPGALGDWMQEPVEPAPPTREASFTAG
                     PPPWQAPGAVWPDPLRGVLYLGGHPGHPRKRKNGTMMFSPNGLEVAGSGFQSWEMNMD
                     WAFVHSMEIQGPDEIMFGDHLKIDSTSSALIITMVDETRMFFEIRTRRPPSLRAALAP
                     VLLMVENIRSHRANG"
     assembly_gap    3763..3862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3863..4737)
                     /locus_tag="PKY13_10330"
     CDS             complement(<3863..4737)
                     /locus_tag="PKY13_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80375.1"
                     /translation="MITLNDAITKRFPQRNEWASEALAALSDDIEPTSLVHEVAEGWM
                     RRGAGATAELIDTLFVLTSDRLGLGRTDRDGDEPRWIELRSIVAVDAVDDSPLPLQTI
                     ELEHGDDLVVCIGWPEAFTERFIGVLVALAGGPDAGESAAETMPRELIDPTALVDDVS
                     DLDSLSPSAWVEGSVAPAADVTVAEPDTVEPIEPIESAAPEASAVTAAELGESPESET
                     GGDRPVVAALDFFNPTPAPEPIAEPLATPEPPAPVPDPFSIDVAAAEAWTSLDRQHDT
                     LPLAPDAFEVAHPPI"
     gene            complement(4876..6039)
                     /locus_tag="PKY13_10335"
     CDS             complement(4876..6039)
                     /locus_tag="PKY13_10335"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOT80376.1"
                     /translation="MEVTSTMALQGNIDSFSVVDVLRLLGSSSKSGRLVVDGDRGSGS
                     LWLGDGSVLGGTTTRTGASAVAAPPSEVLFDVLRFSEGAFVFDAGLEPTELARSGAGP
                     AVDVESMIAEAEEALVEWDDIVAIVPSLRTSISLTLDLAVESVVVDRRLWSGIVAVAA
                     GVATGGDRTTVGAVAELSGLSELPAMRLVRDLVIGGLVDVGAEAPDAPRHTAFDDASF
                     VPFGAPAFEPAGLDGVAFDPPAAPVAEPAAPSYAPPAYEPADLEFPAHQGAPVDVPPP
                     IGNVGVPAERYDAPVADVGESFDAFAPFDRFGTAEPDTGTHDALSDVADPATSPAEAD
                     PVDAAEEAAQQAEFARQLAMLSPRAAEAVASAEAGPSTEDEERARVARFLGSV"
     gene            complement(6149..>7013)
                     /gene="aroE"
                     /locus_tag="PKY13_10340"
     CDS             complement(6149..>7013)
                     /gene="aroE"
                     /locus_tag="PKY13_10340"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOT80377.1"
                     /translation="EGEGRVPEERVLLTPRGERAYAAVIGDPVGHSLSPAIHNAAFSA
                     LGIDARYEAVTVAVGEVPAVVARMRTERWLGMSVTMPHKETVVAELDSLTPIAAELGA
                     VNCVYWVDDDRLGGDNTDGAGVVWALETHVGVAVAGRRVAVLGAGGAARACVAAIAAA
                     GAGEVVVVNRTPERAVQAAALAPAVARTGTIDDLPGADIVINATPVGMAATPGDASVF
                     VPSRGVVAMDLIYHPLETAWLAAARAGGATAVNGVPMLVGQAAVAFERWTGRPAPTDA
                     MLAAVADALDA"
BASE COUNT         1023 a         2441 c         2458 g          991 t
ORIGIN      
        1 gtgaccggct cgacgccgtt cggaccatcg aggacgaacg tgacctcccg cgtcgccttc
       61 ttgtcgcggg cgaacaagcc gatcagctcg tcatcggaca ggccgtcggg cacggtcgaa
      121 cgcagctcgt acgcgccgat gacccggcgg tgctcggcga ccgcggccgc gtcgatgcgc
      181 ccgagacggt acgccacctc ggccgcgtac agcagaccga tggcgaccgc ctcaccgtgg
      241 cggagggcat gatcgctggc ggtctcgatg gcgtgcgcga gggtgtgccc gtagttgagg
      301 atggcccgcc ggcccccctc gcgctcgtcg gacgcaacga catcggcctt gatccgaaca
      361 cacgccgcga cccgttcgtc gagctcgaga gcgtcgaggt cgccgccgcc caaccagtgg
      421 tacttggcca gctcgccgag cccgcaccgc cactcgtcag gcggcagcgt ggcgagcacc
      481 tcggtgtcgc acagcaccgc cgacggctgc caataggcac cgacgaggtt ctttccctcg
      541 ggcaggttga ccccggtctt gcccccgacg gcggcgtcga tctgcgccaa cagcgtcgtg
      601 gccacctgca ccacgggaag gccgcggtgg tacaccgagg cgacgaaccc ggcgatgtcg
      661 gtgacgatgc ctccgccgac accgaccacg gcgtcggcgc gggtcagctc ccaccgggcg
      721 aactgccggc acaggtcctc gaccgtcgcc aaggtcttgt tggtctcgcc gccgcggatc
      781 tcgaacacct cgtggggccg gcccggatcg acggggatgt cgagcccctc ctccgtcacg
      841 atcgcgactc gacgaacagc gggatcgagg acagcggcga gctcgtgacg cacctcgggg
      901 ccgacgagga ccggatacga ccgctcgccg aggtcgacgt cgactcggat catcgggggg
      961 actcctggga ttcggtgtcg ggccagcgtt cggcgatggc ggccagcacc actccggacg
     1021 tgcccgccag gtcgaagaag cccacctcga cggcgaggtc ggcgacctca cgatagaggt
     1081 ggtcacgtcg ctgccggagc gccgcaaggt tcgcctcgag atcgccctcg agcagcggtc
     1141 gccggttgga gctgttgcgg aggcgttcga cgaggactgc gtcggtcgtg tgcagcagta
     1201 cgacccgggc ccggcgggcg agcaggtcgc ggttggtgtg tgactcgacg atgccgccac
     1261 cggtggcaat gacggtgggg tcgggccgtt cgagcgccgc ccgcagcgcg tcggtctcca
     1321 tctgccgaaa ccgcgcttcg ccatcggtgg cgaagatctc ggggatcggc cggccggccg
     1381 catcgacgac cgctgcatcg atgtcgacga cgtcgacgcc gagcatctgt gcgattcgcc
     1441 gggagatcgc cgacttgccg acaccggcca tgccgatgaa cgccaggtgc agcggaggtg
     1501 gactgctcgg ggtcaccgcc cgaggttacc ggtctgggtt tcggcggccg gacccgttgc
     1561 ggagctgctg gttgcagcgt gccgcttgca gcgtctggtg ggcgcgctcg gcgagggtgg
     1621 gtgcgtcgtg cgccgctgcc gcgtagagct cggccgccag ctcgatgtcg ccgttgcgtt
     1681 cgtggaggtg ggccgccacc gcagcgtggc gcgggaccga ttcgtcgacg tcggacaacg
     1741 ccaccagccc tgccggcgca ccatcggcct cgccgaccgc gacggcgcga ttgagccgaa
     1801 cgaccggcgt gtcggtgtac cgcaacagct cgtcgtacca ctcgacgatc tgcacccaat
     1861 cggtctcctc gacccgcggc gcgtcggcgt gcagcgcggc gatcgccgcc tgcgcctggt
     1921 actcgccgag ccgatcgcgg gccagggccg cctggaggac gtcgacaccc tcggcgatga
     1981 gctcggtgtc ccactggctc cgatcctgtt cggcgagcgg aaccagacgg ccgtcgggcc
     2041 cggtgcggct cgttcgtcgg gcgtggtgca ggagcatcag ggcgagcaac ccgtggacct
     2101 ccggatcgtc ggcgagacgg gacaactgcc gggtcagccg gatcgcctcg gccgcgagat
     2161 cgacgtcgcc ggagtacccc tcgttgaaga ccaggtacaa cactcgcatc accgtgctgt
     2221 ccgacaccgt gctgtccgac gccgtgctgc ccgacgactc tcttggcgcg gctgatgcgc
     2281 tgcgccatcg tcgcctcggg caccaggtac gccctggcga tctggcgggt ggtgaggcca
     2341 cccacggcgc gaagggtcag cgcgacggcc gacgacggag tcaggtccgg gtgggcgcac
     2401 aggaagtaca gctgaagggt gtcgtcgacg ttcgggacgg cgccggttgg aggttcggtg
     2461 tcgaaccgga cctcccggtc gcggcgggcg ctctcggacc gggtcaggtc gaggaacgcc
     2521 cgccacgcca cggtgatcag ccaggcggtg gggtccgacg gcggcgaact ctgccaggcc
     2581 ccgagcgccc ggatcagcgc ctcctgcacc gcgtcctcgg cagtggcgaa gtccgcgccc
     2641 cggccgacga gcacccccag cacccgcggg acgagcacgc gcagctcgac gtcggcatcc
     2701 atcggagcgg tcagtcggtg atcgtcgggg gcgccgtcag gaacggccgg acctcgagcc
     2761 actcatggat cggctcgccg ttggcgcccg gcgccgacga cagctcaccg gccaactcga
     2821 tcgcccgtgc ctcgctgtcg acgtcgatga tcatccagcc cgcgatgagg tccttggtct
     2881 cggcgaacgg gccgtcggtg accggcggac gccccgcgcc gtcggaacgc acccacatgc
     2941 cttccggcgc cagcgcctgc ccgtcgacga actcacccga cgcctcgagg cgggcggcga
     3001 agtcacccat gaactggatg tgggcgtcga tctcgtcggg cgtccagcgc tccatcggga
     3061 tgttgttggc gggggcgggg gcgccccggt agtgcttcag caacaggtac ttggccatcg
     3121 tgttctcctc gtgtgaggcc ggcggtgccg gctggttcac ccaggtgacg gaacaggccc
     3181 ggcgttctcg acatcctcgc cgagaaatgt ccgtgtcgac tgcgaccgac gatcagccgt
     3241 tggcgcggtg gctccggatg ttctcgacca tgaggagcac cggggcgagc gccgcacgca
     3301 acgacggagg gcgccgggtg cggatctcga agaacatccg cgtctcgtcg accatcgtga
     3361 tgatcagggc gctgctggtg gagtcgatct tgaggtgatc gccgaacatg atctcgtcgg
     3421 gcccctggat ctccatgctg tgcacgaacg cccagtccat gttcatctcc cagctctgga
     3481 agccggagcc ggccacttcg agcccgttcg gggagaacat catcgtgccg ttcttgcgct
     3541 tccgggggtg cccgggatgg ccaccgaggt acaacacgcc gcgcaacggg tcgggccaca
     3601 ccgctccggg ggcctgccac ggcggtgggc cggcggtgaa ggacgcttcg cgtgtcggcg
     3661 gtgccggctc gaccggctcc tgcatccagt cgcccagtgc acccgggtcg aaggtgcccg
     3721 acgccgcctc gaacgggccg gtctcgacgg gaaccgtctc gannnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nntggatcgg cgggtgggcg acctcgaagg cgtcgggcgc
     3901 gagcgggagg gtgtcgtgct gacgatcgag ggacgtccac gcctcggcag cggcgacatc
     3961 gatcgagaac gggtccggca ccggcgccgg cggctcgggc gtcgccagcg gctccgcgat
     4021 cggctcggga gcgggcgtgg ggttgaagaa gtcgagcgct gcgacgaccg ggcgatcgcc
     4081 accggtctcg gactcgggcg actcccccag ttcggctgcg gtcacggccg acgcctcggg
     4141 tgccgccgac tcgatcggtt cgatcggttc gaccgtgtcg ggttcggcga ccgtgacgtc
     4201 ggccgcgggc gccaccgacc cctcgaccca cgcgctggga gacaacgagt cgaggtcgga
     4261 cacgtcatcg acgagcgccg tcgggtcgat caactcccgc ggcatcgtct cggcggcact
     4321 ctcaccggca tcgggacccc cagcgagggc cacgagcaca ccgatgaagc gttcggtgaa
     4381 ggcttcgggc cacccgatgc agacgacgag gtcgtcgccg tgctccagct cgatggtctg
     4441 cagcggcagc ggcgagtcgt cgacggcgtc gacagcgacg atcgatcgca gctcgatcca
     4501 ccggggctca tcgccgtcac ggtcggtgcg gcccaggccc agtcgatcgc tcgtgagcac
     4561 gaacagcgtg tcgatcagct ccgcagtggc gccggcgccg cggcgcatcc acccctccgc
     4621 cacctcgtgg acgagggacg tgggctcgat gtcgtcggag agcgcggcga gcgcctccga
     4681 tgcccactcg ttcctctgcg ggaaacgctt cgtgatcgcg tcgttcaatg tgatcatcgg
     4741 gtcaccatcg cgccctcggc gctgttctcg agtgctgttg ctgtcggtgt tgtcgggcga
     4801 cgccacgcgg cggctcctga aaggatcgga atctcgctgg acgtcgggga atgatctgtt
     4861 cgggtcgtca tcgaatcaga cggatcccag gaatctagcc actctggccc gctcctcgtc
     4921 ctcggtcgat gggccggcct cggcgctggc caccgcttcg gcggcgcgag ggctcaacat
     4981 ggcgagctga cgggcgaact ccgcctgttg cgcggcctcc tcggccgcgt cgacgggatc
     5041 ggcctcggcc ggcgacgtcg caggatcggc gacgtcggac agcgcatcgt gggtgccggt
     5101 gtcgggctcc gcggtgccga accggtcgaa gggagcgaac gcgtcgaacg actcaccgac
     5161 gtccgcgacc ggggcgtcgt agcgctcggc gggaacgccg acgttgccga tgggtggcgg
     5221 gacgtcgacg ggggcgccct ggtgagcggg aaactcgaga tcggcgggct cgtaggccgg
     5281 gggtgcgtac gagggcgcgg caggctcggc cacgggcgcc gccggcgggt cgaacgcgac
     5341 gccgtccagg ccggccggct cgaaggccgg cgcgccgaac ggcacgaacg acgcgtcgtc
     5401 gaaggcggtg tgccgaggcg catcgggagc ctcggccccc acgtcgacga ggccgccgat
     5461 cacgaggtcg cggaccagac gcatcgccgg gagctcgctg agccccgaca actcggcgac
     5521 ggcaccgacc gtcgtacggt cgccgccggt ggcgacaccg gccgccaccg cgacgattcc
     5581 cgaccacagc ctccgatcga ccaccaccga ttccacagcg aggtcgagcg tcagcgagat
     5641 gctcgtgcgc aacgacggca cgatcgcaac gatgtcgtcc cactcgacga gggcctcctc
     5701 ggcctcggcg atcatcgact cgacgtcgac ggccggaccg gcccccgagc gagccagttc
     5761 ggtcggctcc agccccgcgt cgaacacgaa ggcaccctcg ctgaaccgca gcacgtcgaa
     5821 caggacctcc gacggcgggg ccgccacggc cgacgcgccc gtccgcgtcg tggtgccacc
     5881 gaggacgctg ccatcgccca accagaggct gccgctcccg cggtcaccgt cgacgaccag
     5941 gcgccccgat ttcgaggacg aaccgaggag ccgcaggacg tccaccacgg agaacgagtc
     6001 gatgttgccc tgtagtgcca tggtgctggt gacctccacc ggtgagaacg ttcggactcc
     6061 tatcggcacc tttcaccgaa tcatgaacgc gttctgggcc aaatccccca ctcgatccct
     6121 gccggatggc ccggtgcggg aaccgtgctc aggcgtcgag ggcgtcggcg acggccgcca
     6181 gcatggcgtc ggtcggagcc ggcctgccgg tccaccgttc gaaggccacc gccgcctggc
     6241 cgacgagcat cgggacgccg ttcaccgcgg tcgcaccacc ggcccgcgcc gccgccaacc
     6301 acgcggtctc gagagggtgg tagatcaggt ccatcgccac gacaccgcgg ctgggcacga
     6361 agacgctcgc gtcgccggga gtcgccgcca tcccgaccgg cgtcgcgttg atcacgatgt
     6421 ccgcgcccgg caggtcgtcg attgtgccgg tgcgcgccac cgcaggggcg agcgccgctg
     6481 cctgcacggc gcgttcgggg gtgcggttga cgacgaccac ctcgccggcg ccggccgcgg
     6541 cgatcgcggc cacgcatgcc cgggcggcac cgccggcgcc gagcaccgcc actcggcggc
     6601 cggcgacagc gacaccgacg tgggtctcga gggcccacac cacgccggca ccgtcggtgt
     6661 tgtcgccgcc cagccggtcg tcgtcgaccc agtacacgca gttgaccgca ccgagctcgg
     6721 ccgcgatggg cgtgagcgag tccagctcgg cgacgacggt ctccttgtgc ggcatcgtca
     6781 ccgacatccc caaccagcgc tccgtgcgca tgcgggccac gacggcggga acctccccga
     6841 ccgctaccgt caccgcctcg taccgggcgt cgatgccaag ggcggagaag gccgcgttgt
     6901 ggatggccgg agagagcgag tgaccgaccg ggtcgccgat gacggccgcg taggcccgct
     6961 caccccgagg ggtcaacaga acccgttctt ctggcactct tccttcgcct tct
//
LOCUS       DAOSOA010000156         6995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10826_length_6995_cov_6.503314, whole genome shotgun sequence.
ACCESSION   DAOSOA010000156 DAOSOA010000000
VERSION     DAOSOA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10826_length_6995_cov_6.503314"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKY13_10345"
     CDS             complement(<1..252)
                     /locus_tag="PKY13_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80378.1"
                     /translation="MPTGQVVVTGILQPDETFYADAASLPGTVASIAHDRLAELWGTT
                     VLPGFTVLAAQEPATEPAPVPVQPTVQTADVPFPLQNFAY"
     gene            263..541
                     /locus_tag="PKY13_10350"
     CDS             263..541
                     /locus_tag="PKY13_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80379.1"
                     /translation="MPYTIGSTVVEFEQDRRIAWAHIGGHRWRYELTELEPVDGDPRT
                     EVVETFDWSTARVPKAIELVGYPTKHVPNMERTLEKLDAFVTNRASAL"
     gene            546..1166
                     /locus_tag="PKY13_10355"
     CDS             546..1166
                     /locus_tag="PKY13_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HOT80380.1"
                     /translation="MSDDSVRRVAAWDFDGTITERDTLVGFLEFVGGRAALARAFAAE
                     SPRLARGARSSTQRNAAKEGVLGRILGGRREDDIVEAGIRYANRLPRGFRRSSIERIA
                     HHRTSGHDLVIVSASLVYYLRPIASDLGFHHVIAVEMEADTDGVLTGALTTPNVRREH
                     KETRLREWLGDEPFELWAYGDSSGDEHLLGMAHHPTWVGRRAKQND"
     gene            complement(1179..1952)
                     /locus_tag="PKY13_10360"
     CDS             complement(1179..1952)
                     /locus_tag="PKY13_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT80381.1"
                     /translation="MEITGSSAVVTGGASGLGEATSKLLAAKGMKVVVMDLGHQQEKG
                     DALAKEIGGLFVAADVTDAEQVQAAIDAAVEMGPLKALVNCAGIGWASRTIGKNGEPH
                     DLDIFRRVIEINLIGTFNCIRLAASAMNANEGDADGAKGAIVNTASVAAYDGQIGQAA
                     YSASKGGVVGMTLTIARDLGAAGIRVNTIAPGLIDTPIYGEGEHADAFKAKLGESVLF
                     PRRLGVSEEFASLALELITNSYINAQDIRIDGGIRMPPK"
     gene            2082..2567
                     /locus_tag="PKY13_10365"
     CDS             2082..2567
                     /locus_tag="PKY13_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOT80382.1"
                     /translation="MDQMRDGGVAGRAARSFPGGQVRLPRLNEDRSKGPQIDMVDTAF
                     IGGGTATVLKEEPSGFTGYYTYESLFDEPAEGDDDFRGESPYDVLRVAPTASWSEIVT
                     AHRTLVKEFHPDRFVDHPAEVVAQAEVEIKRINRAYGELQQLRTSSDRRTGGDRRQNP
                     R"
     gene            complement(2574..3107)
                     /locus_tag="PKY13_10370"
     CDS             complement(2574..3107)
                     /locus_tag="PKY13_10370"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HOT80383.1"
                     /translation="MSLTTEPGTIRVVYTDGACRGNPGPGGWAWALDEDRFASGFDVD
                     TTNQRMEILAAYQAVRSLDGLLEVRSDSTYVVNCFVKQWHRGWHSRGWLNSQKQPVAN
                     RDLWEPFIDLVLGRGDVRFTWVKGHSTDTMNQLVDRLAVEASRVGEGRTGGDFEFPVA
                     PERPVPVAPAAVQSQLF"
     gene            3169..5877
                     /gene="aceE"
                     /locus_tag="PKY13_10375"
     CDS             3169..5877
                     /gene="aceE"
                     /locus_tag="PKY13_10375"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOT80384.1"
                     /translation="MSSDGFLQQLPDVDPIETAEWVDSLEAVATKLGAPRASFLLARI
                     EARAEALGIPNSGLLTTPYLNTIPPADQPWFPGDEDIERRIRAFIRWNAVAMVVRANH
                     AADGIGGHLSTFASSAALYDVGFNHFFRGKDDGNAGDHIYIQGHAAPGIYARAFLEGR
                     LSESQLDRFRREIGGGGLSSYPHPRLMPDFWEFPTVSMGLGPINSIYQARFNRYLHNR
                     RIDATDSSRVWCFVGDGECDEPETLGSIALAGREGLDNLTWVVNCNLQRLDGPVRGNA
                     KIIQELEGVFRGAGWNVVKVVWGTPWDELIAQDFDGALVHKMNTTVDGEFQRYAVEDG
                     SYAREHFFGPDTRLRQLVEHMSDEEIAALPRGGHDYRKLYAAYHWATHNSTGKPTVIL
                     AKTIKGWTLGSEVMGRNATHQIKKMSAVQLRTLRDRLQLGDHISDEDLADGEPPFARP
                     AEGSPEHDYLMARRKALGGHIPSRRTTPRRPLAQPEASTFAELLAGSGGQAASTTTAF
                     TRLLRNLSRDPNVGDRIVPIVPDEARTFGMDSLFRDLKIYAAQGQKYEPVDYHLLLTY
                     AESSDGQILEEGITEAGSMASWTAAATSYATRGVPMIPFFIFYSMFGFQRVGDLIWAA
                     ADARARGFLLGATAGRTTLLGEGLQHQDGHSLVLASTVPSCRAYDPAFAYEMAILVHD
                     GIQRMYRDGEDVFYYLTLYNENYPMPAMPEGVEPEVVAQGVVDGLYRWKAAPEGLAHP
                     ATILFSGTGWTAADEARTVLADDYGVGAELWSATSYKALREQALEVERWNRLHPTEPD
                     RVPQVVELLGQSAGPIVAVSDFVRAVPDQIARWAPRPWLALGTDGFGRSDDRAALRRF
                     FETDTGHVVVAVLSKLADAGEIEPKVVQSAIERFEIDSESRAPWVD"
     gene            5968..6555
                     /locus_tag="PKY13_10380"
     CDS             5968..6555
                     /locus_tag="PKY13_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HOT80385.1"
                     /translation="MATKKATIPITNTPEANQLLVEDPLALLLGMLLDQQVPMEWAFL
                     GPYTLKERLGGKLDAHTIAAMDRDEFVTICCDKPAIHRFPGSMGKRIWDVCAFLVEHY
                     DGNAAKIWNRVPSGDELYRRLREVPGYGDEKAKIFMAILAKRFGKQPAGWEEAAAPFS
                     DSQPRSVADVADAATLAQVREWKKAKKAAAKTKQD"
     gene            6724..>6995
                     /locus_tag="PKY13_10385"
     CDS             6724..>6995
                     /locus_tag="PKY13_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80386.1"
                     /translation="MLPDRGPARLAAWGAVAVAVALLHNRLWATFNLAFFTAISKNLG
                     RNPFGNGFDGDYLLTNLTGPTLARLLHQTEPHEYARLHLVVLLLGLG"
BASE COUNT         1170 a         2474 c         2326 g         1025 t
ORIGIN      
        1 gtacgcgaag ttctgcagcg ggaacggcac gtcggcggtc tgcacggtcg gctggaccgg
       61 aacgggggcc ggctccgtgg ccggctcctg cgcggcgagg accgtgaacc ccgggagcac
      121 cgtggtcccc cagagctcgg ccagccgatc gtgcgcgatg gaggcgacgg tgccgggcag
      181 cgaggccgcg tcggcgtaga aggtctcatc gggctgcagg atgcctgtga cgaccacctg
      241 gccggtgggc atgcgcttgg gcgtgccgta cacgatcggc agcaccgtcg tggagttcga
      301 acaggatcgc cgtatcgcgt gggcccacat cggcggacac cgctggcgct acgagctgac
      361 cgagctcgag cccgtcgacg gcgatccccg caccgaggtc gtggagacgt tcgactggtc
      421 gacggctcgt gtcccgaagg cgatcgagct cgtcgggtac cccaccaagc acgtacccaa
      481 catggaacgg accctggaga agctcgacgc gttcgtcacc aaccgggcga gcgccctgta
      541 gtcacatgag cgacgactcc gtgcgacggg tcgcggcctg ggacttcgac ggcacgatca
      601 ccgagcgcga caccctggtc ggcttcctcg aattcgtcgg tgggcgcgcc gcgctggcac
      661 gggcgttcgc cgccgagtcg ccgcgcctgg cccgcggcgc ccggtcgagc acgcaacgca
      721 acgccgcgaa ggaaggcgtc ctcgggcgca tcctcggcgg acgccgggag gacgacatcg
      781 tcgaggcggg catccgctac gcgaaccggt tgccccgcgg cttccgacgg tcgtcgatcg
      841 aacggatcgc ccaccaccgc acgagcgggc acgacctcgt gatcgtgagc gcatcgctcg
      901 tgtactacct gcgccccatc gcgtccgacc tggggttcca ccacgtgatc gccgtcgaga
      961 tggaggccga caccgacggc gtcctcaccg gtgcgctcac cacgcccaac gtcaggcggg
     1021 agcacaagga gacgcggctc cgggagtggc tcggcgacga gccgttcgag ctctgggcct
     1081 acggcgacag ctcgggcgac gagcacctgc tcggcatggc ccatcacccc acctgggtgg
     1141 ggcgacgggc caagcagaac gactgactca gactcgcatc acttcggggg catccggatg
     1201 ccgccgtcga tgcggatgtc ctgggcgttg atgtagctgt tcgtgatcag ctcgagcgcg
     1261 agcgacgcga actcctcgga cacaccgagc cggcgcggga agaggaccga ctcaccgagc
     1321 ttcgccttga aggcatcggc gtgctcaccc tcgccgtaga tcggggtatc gatgaggccg
     1381 ggggcgatgg tgttgacccg gatgccggcg gcgccgaggt cgcgggcgat ggtcagcgtc
     1441 atgccgacga cgccaccctt ggacgccgag taggcggcct ggccgatctg gccgtcgtag
     1501 gcggcgaccg aggcggtgtt gacgatggcg cccttggcac cgtcggcgtc accctcgttg
     1561 gcgttcatcg ccgaggccgc gaggcggatg cagttgaacg tgccgatgag gttgatctcg
     1621 atgactcgac ggaagatgtc gaggtcgtgc ggctcgccgt tcttgccgat ggtccggctg
     1681 gcccaaccga tgccggcgca gttgaccagc gccttgagcg gacccatttc gacggcggcg
     1741 tcgatggccg cctggacctg ctcagcgtcg gtgacgtcgg cggcgacgaa caggccaccg
     1801 atttccttgg cgagcgcatc gcccttctcc tgctggtggc cgaggtccat gaccacgacc
     1861 ttcatgccct tcgccgccag gagcttggac gtggcctcgc cgaggcccga cgcaccaccc
     1921 gtgacgactg ctgatgatcc cgtgatttcc atggggggaa gcctaggcgt tgaccggtcg
     1981 gtcaagtcca gtccggcacc gtcgtgcccg tcggggggaa ggcggagaag tcccgcgccg
     2041 agaaggtgaa gtttcgcgac ggttcgaccg atacaggtcc tatggaccag atgcgtgacg
     2101 gcggtgtagc agggcgggca gctcgatcgt tccccggcgg gcaggtccgg ctgccgcgtc
     2161 tgaacgagga ccggtcgaag ggcccgcaga tcgacatggt cgacacggcc ttcatcggcg
     2221 gcgggaccgc cacggtgctc aaggaggaac cgagcgggtt caccgggtac tacacctacg
     2281 agtcgctgtt cgacgagccg gccgaaggcg acgacgactt ccgcggcgag agcccctacg
     2341 acgtgctcag agtcgcaccg accgccagct ggtccgagat cgtgaccgcg caccgcaccc
     2401 tcgtgaagga attccacccc gaccgcttcg tcgatcaccc cgccgaggtc gtggcgcagg
     2461 ccgaggtcga gatcaaacgg atcaaccggg cgtacggtga gctgcagcag ctgcgcacga
     2521 gctctgaccg ccgcaccggc ggagaccggc gccagaaccc gcgctgaccc aggtcagaag
     2581 agctgggact gcacggccgc cggcgcaacc ggcacggggc gttcgggtgc caccgggaac
     2641 tcgaagtcgc cacccgtgcg gccctcacca acacgcgatg cctcgacggc gagccggtcg
     2701 accagctggt tcatcgtgtc ggtgctgtgt cccttgaccc acgtgaagcg gacgtcgccg
     2761 cgcccgagga cgagatcgat gaacggctcc cacaggtcac ggttggcaac gggctgcttc
     2821 tgggagttga gccacccccg cgagtgccat ccgcggtgcc actgcttcac gaagcagttc
     2881 acgacgtagg tcgaatcact ccggacctcg agcaggccgt cgagcgagcg cactgcctgg
     2941 tacgcggcga ggatctccat ccgctggttc gtcgtgtcca cgtcgaaccc ggacgcgaag
     3001 cgatcctcgt cgagcgccca cgcccacccg ccgggtccgg gattcccgcg gcacgcgcca
     3061 tcggtgtaga cgacccggat ggtcccgggc tcggtggtga ggctcacccg ggggaccgta
     3121 gtcgtaaggt gggccgcaac tgaacgatcg cggaggttcc ccccgaccat gagctccgac
     3181 ggcttcctgc aacagctgcc cgacgtcgat cccatcgaga ctgccgaatg ggtcgactcg
     3241 ctcgaagccg tcgccaccaa gctcggcgcc ccccgcgcca gcttcctcct ggcacggatc
     3301 gaagcgcgcg ccgaggcatt gggcatcccc aactccggcc tcctgaccac cccgtacctc
     3361 aacaccatcc cgccggccga ccagccgtgg ttccccggcg acgaggacat cgagcgacgt
     3421 atccgagcct tcatccgctg gaacgcggtg gccatggtgg ttcgggcgaa ccacgccgcc
     3481 gacggcatcg gcggccacct ctcgacgttc gccagctccg cagcgctgta cgacgtcggg
     3541 ttcaaccact tcttccgcgg caaggacgac ggcaacgccg gcgaccacat ctacatccag
     3601 ggccacgcgg cacccggcat ctatgcccgc gcgttcctcg agggtcgcct gagcgaatcc
     3661 cagctcgacc gcttccgccg tgagatcggc ggcggcggcc tctcgagcta tccccacccc
     3721 cgcctgatgc ccgacttctg ggagttcccg acagtgtcga tggggttggg cccgatcaac
     3781 tcgatctacc aggcccgttt caaccgctac ctgcacaatc gccgcatcga cgcgaccgat
     3841 tcgtcgcgag tgtggtgctt cgtcggcgac ggcgagtgcg acgaacccga gacgctcggc
     3901 tcgatcgcgc tggcaggccg cgagggcctc gacaacctca cctgggtcgt caactgcaac
     3961 ctgcaacgcc tcgacgggcc ggtgcgcggc aacgccaaga tcatccagga gctcgaaggg
     4021 gtgttccgcg gcgccgggtg gaacgtcgtc aaggtcgtgt ggggcacgcc gtgggacgag
     4081 ctgatcgccc aggacttcga cggcgcgctg gtgcacaaga tgaacaccac cgtcgacggc
     4141 gagttccagc ggtacgcggt cgaggacggc agctacgccc gcgagcactt cttcggaccc
     4201 gacacccgcc tgcgccaact cgttgagcac atgagcgacg aggagatcgc agcgctcccc
     4261 cgcggcggcc atgactaccg caagctctac gccgcctacc actgggccac ccacaactcg
     4321 acgggcaagc cgaccgtcat cctcgccaag accatcaagg ggtggacgct cggcagcgag
     4381 gtgatgggcc gcaacgccac ccatcagatc aagaagatgt cggcggttca gttgcgaacg
     4441 ctgcgcgacc gcctccagct cggggaccac atcagcgacg aggacctcgc ggacggtgag
     4501 ccgccgttcg cccgacccgc cgagggctcc cccgagcacg actacctgat ggcgcgacgc
     4561 aaggcactcg gcggccacat cccgtcgcgg cgcaccacgc cccggcgccc cctcgcccaa
     4621 ccggaggcct cgacgttcgc cgagctgctc gccggttccg gcggccaggc ggcgagcacg
     4681 accacggcgt tcacccgcct cctccgcaac ttgtcacgtg atcccaacgt cggcgaccga
     4741 atcgtcccga tcgtgcccga cgaggcacgc acattcggca tggactcgtt gttccgcgac
     4801 ctcaagatct acgccgctca gggccagaag tacgagccgg tcgactacca cctcctcctg
     4861 acctacgccg agtcgtccga cggccagatc ctggaagagg gcatcaccga ggccgggtcc
     4921 atggcgtcgt ggaccgccgc ggcgacgagc tacgcgaccc gcggcgtccc gatgatcccg
     4981 ttcttcatct tctattcgat gttcgggttc caacgggtgg gtgatctcat ctgggcggcg
     5041 gcggacgccc gggcccgcgg cttcctcctg ggcgccacgg ccgggcgcac caccctcctc
     5101 ggcgagggcc tccagcacca ggacggccac agtctggtgc tcgcttcgac ggttccgtcc
     5161 tgtcgggcgt acgaccccgc gttcgcctac gagatggcga tcctcgtgca cgacggcatc
     5221 cagcgcatgt accgcgacgg cgaggacgtc ttctactacc tcacgctcta caacgagaac
     5281 taccccatgc cggccatgcc cgagggtgtc gaacccgagg tcgtggccca aggcgtcgtc
     5341 gacggcctgt accgctggaa agctgccccg gagggtctcg cacacccggc cacgatcctg
     5401 ttctcgggca ccggctggac cgccgcggac gaggcgcgca ccgtgctcgc cgacgactac
     5461 ggcgtcggcg ccgagctgtg gagcgcgacg agctacaagg cgctgcggga gcaggcgctc
     5521 gaggtcgaac ggtggaaccg gctgcacccg accgaacccg atcgggtgcc acaggtggtg
     5581 gagctgctcg ggcagtcggc cggtccgatc gtcgcggtgt ccgacttcgt ccgtgccgtt
     5641 cccgaccaga tcgctcggtg ggcgccgcgc ccgtggctgg cgctcggcac cgacgggttc
     5701 ggccgctccg acgaccgcgc cgcgctccgt cgcttcttcg agaccgacac cggccacgtc
     5761 gtcgtcgccg tgctcagcaa gctcgccgac gccggcgaga tcgagccgaa ggtcgtgcag
     5821 tcggccatcg agcgcttcga gatcgacagc gagagcagag cgccgtgggt tgactaaccg
     5881 aggggttttg tgttgcgagg agcgacgcca gcgtcgctcc tcgcactaca aaaccccgaa
     5941 gttgggatcg ttctaggctg ccgggccatg gcgacgaaga aggcgacgat cccgatcacc
     6001 aacacccccg aggccaacca actcctggtc gaggatccgt tggcgctgct tctcggcatg
     6061 ctgctcgacc agcaggtccc gatggaatgg gcgttcctcg gtccgtacac cctgaaggag
     6121 cgcctcggcg gcaagctcga cgcccacacg atcgcggcga tggaccgcga cgagttcgtc
     6181 accatttgtt gcgacaagcc agccatccac cgcttccccg gctcgatggg caagcgcatc
     6241 tgggacgtgt gcgccttcct cgtcgagcac tacgacggca acgcggcgaa gatctggaac
     6301 cgggtcccgt ccggcgacga gctctaccgt cgcctgcgcg aggtaccggg ctacggcgac
     6361 gagaaggcca agatcttcat ggcgatcctc gcgaagcggt tcggcaagca gccggccggt
     6421 tgggaggaag cggcggcccc gttcagcgac tcgcagccgc gctcggtggc cgacgtcgcc
     6481 gacgctgcca cactcgccca ggtgcgggag tggaagaagg ccaagaaggc cgccgccaag
     6541 accaaacagg actgagcgcc ggaagatcag cccgagcaga tcgggaacgg cacccggccg
     6601 gcgatcccga ggaagatgcc cgcgagaccc cgacggtcgt cccgcaggaa cgaccttggt
     6661 gacagagtgt caccaacgtc gagatggcgt gacccggaga accgatggtg taggaaggca
     6721 cgcgtgctcc ccgaccgcgg acccgcccgc ctcgccgcct ggggcgccgt cgccgtggcg
     6781 gtcgcgctcc tgcacaatcg cctgtgggcg acgttcaacc tggcgttctt cacggcgatc
     6841 tcgaagaacc tgggccgcaa ccccttcggc aacggcttcg acggcgacta cctgctgacc
     6901 aacctcaccg gcccgacact cgcccgcctc ctgcaccaga ccgagccgca tgagtacgcc
     6961 cggctccacc tcgtggtgct cctcctcggc ctcgg
//
LOCUS       DAOSOA010000157         6981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10863_length_6981_cov_6.972712, whole genome shotgun sequence.
ACCESSION   DAOSOA010000157 DAOSOA010000000
VERSION     DAOSOA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10863_length_6981_cov_6.972712"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKY13_10390"
     CDS             <1..411
                     /locus_tag="PKY13_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOT80387.1"
                     /translation="PPAMLFDEAGPPGPHANLVRFRLGKADGVTFTLNAKQPGKDLDS
                     EPIDIAVDFAAALGVRAEAYERLLDDALDGNPRRFGRYDVVEETWRIVQPALDAPGPV
                     HPYARGSWGPAIADQLVGSEGWYEPSIGTEARSS"
     gene            408..1097
                     /gene="pgl"
                     /locus_tag="PKY13_10395"
     CDS             408..1097
                     /gene="pgl"
                     /locus_tag="PKY13_10395"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOT80388.1"
                     /translation="MIQFRYVATEEFASAGADLMATHITRAIDDRGACHLALSGGRAP
                     WELFGVLARTDLPWSKVHVWQVDERVAPDRDVDRNSVGLKTSLLALAPILASNVHLMD
                     VTAPDLGEAAAAYAADLARLCDSTLDLVHLGLGADGHTASWPPGDPVIDIDDRDVAIS
                     KLYNGFVRMTLTVPCINRARHRVFLITGGDKEHAINELGAMGDIPASRVSVDGTDALV
                     ALPMPLLQELD"
     gene            complement(1122..1730)
                     /locus_tag="PKY13_10400"
     CDS             complement(1122..1730)
                     /locus_tag="PKY13_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80389.1"
                     /translation="MHRRETHRRRFRRARLIAGITLAAVVTAGVAGCGDEPRDGDSAV
                     AVTASTVAPGGVNALADPEKVPILIREHLGESPEIRRLSLNSNGFSVQVRDPAKRDNM
                     DDYDFYNGTWTTRPVSVSVSEIEAYESVTFYLGDISWEKIPGLIDQALTGLDLEGEEV
                     GNVGFDRLAGERPRVYIGVNGLRGNGRLIARADGTEVDIQRN"
     gene            complement(1682..2131)
                     /locus_tag="PKY13_10405"
     CDS             complement(1682..2131)
                     /locus_tag="PKY13_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT80390.1"
                     /translation="MSAPRNKSDLADAAWSSVLAFFLARKEVLVEVAVEFELTPGDLQ
                     ALMSLDPDEPKPMKVLAETFRCDPSNATWMVDRLEQRGIVERQSLPHDRRVRAVAVTT
                     DGRLLRDSVMARLSTAPPEFAALSTTELDTLRCIVAKLTDAGSDERG"
     gene            2163..2399
                     /locus_tag="PKY13_10410"
     CDS             2163..2399
                     /locus_tag="PKY13_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80391.1"
                     /translation="MSLGERETDDEVAGPEGPPRWPAPSSRPVGSHHAYPAPDSEAPA
                     GSGTSGPSTTVIAVCLGLGISLFALGVALMTIVL"
     gene            2437..3024
                     /locus_tag="PKY13_10415"
     CDS             2437..3024
                     /locus_tag="PKY13_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOT80392.1"
                     /translation="MTELIGALGGLGLASSAGLNAYIPALLTALLARYTDLVTLPKEF
                     EFLSSGWVLITLTVLLVIEEVVDKVPGADHLNDVVQTVVRPVSGAVLFAAGSEDAFGR
                     HTWLALGVGFVAALGVHATKAAGRGVVNVSTGGIGAPVVSVIEDIVSLTGAVVAIVAP
                     VLVILFVIAFVWLLVVILRKRRERRERKAAAAVPT"
     gene            3073..4710
                     /locus_tag="PKY13_10420"
     CDS             3073..4710
                     /locus_tag="PKY13_10420"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOT80393.1"
                     /translation="MTEFAFSEILPVSGDSTPYRLLTTEGVSTFEANGNRFLQVEPEV
                     LTTLTATAMRDIAHLLRPGHLQQLRNILDDPEASSNDCFVALELLKNANIAAGGVLPS
                     CQDTGTAIIKGKKGQFVVVNGGHDEEHLARGVFDTYQTSNLRYSQMAPLTMWDEVNTA
                     SNLPAEIKISAIDGDAYKFMFMAKGGGSANKSYLFQETKALLNPKSLMAFLDKNLRYI
                     GTSACPPYHLAIVIGGTSAEFAVETAKLASTRYLDDLPKQGSPTGHGFRDVELEAEVL
                     ALTQSFGIGAQFGGKYFCHDVRVIRLPRHGASCPVAIAVSCSADRQALAKITADGVFL
                     EQLEHDPARFLPEVNEAHIGGEVISIDLSRPMDEIRAELTRYPVKTRLSLNGTLVVAR
                     DIAHAKIAERLDAGEEMPSYLRDHAVYYAGPAKTPSGMASGSFGPTTAGRMDSYVDKF
                     QAAGGSMVMLAKGNRSQVVTDACNTHGGFYLGSIGGPAAKLAQDCIRHVEVLDYPELG
                     MEAVWKIEVEDFPAFIVVDDKGNDFYADVSTPVALRK"
     gene            complement(4731..5756)
                     /locus_tag="PKY13_10425"
     CDS             complement(4731..5756)
                     /locus_tag="PKY13_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80394.1"
                     /translation="MARRRRETNAEIATTTPERRGNRTPDFLRPNEEPAAKVADAEQR
                     SAAATSSDADTPTTTVTATATKNGDAADDDLRDRLASLQTMVEQLVAAQVSPVATSGD
                     PEVDSASRTLRFAQQTADSVIAEAREEALAIVADAERQRSEIIRRARQQADQDYTAER
                     DEILAASAAWQAQRSEILEQLDALTAVFGRYHDGLADLGGTVDAVGTRLRTGIAAEPI
                     VIASAANDAEPVGDDESDGRSDGTTVDHASADDTAAEVADAEVVEPADSDDPVVEAVG
                     DVTNEGDVIDLTRRNPFTRAPVSKVQKIDADTDPFVFDVAELPETTGDFGWETDPVDG
                     PDGATTF"
     gene            5940..>6981
                     /locus_tag="PKY13_10430"
     CDS             5940..>6981
                     /locus_tag="PKY13_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80395.1"
                     /translation="MVTNTSSHSGGGPKSQLAADIIAVGSTLRGLVSGLEPKDVPLPF
                     APEVLAAFADVQRVAESGRVLMTARAAQAREWERQGYASAADWLAAQQGTTTGRARAD
                     LETSGHLDNLEATADAVRAGRLSPEAAGAVADAAAANPGAEDDLLDLAQRESLKRTRQ
                     EAERRKAEVRSEDEKRQREERVRKDRSLRFWFGNGAGNLHATGPIDVLKELEALIQRE
                     VDRKFRANRDAASRESRDNYAFDALIDLARTGGTGANKVGEPAEDRAESAGSASSAGR
                     ARRAPLTRLTLIRVDLAALLRGSVAHGEVCEIGGIAVSVAELRQLLGESIMQLVLTNG
                     EAVIDVVNLGRKP"
BASE COUNT         1032 a         2433 c         2399 g         1117 t
ORIGIN      
        1 cccccggcga tgctgttcga cgaggcgggc cctcccggcc cccacgccaa cctcgtgcgc
       61 ttccggttgg gcaaggccga cggcgttacc ttcacgctca acgccaagca gccgggcaag
      121 gacctcgatt ccgaaccgat cgacatcgcc gtcgacttcg ccgcggccct gggcgtacga
      181 gccgaggcct acgaacgcct gctcgacgac gcgctcgacg gcaacccgcg ccgcttcggc
      241 cgctacgacg tggtcgagga gacctggcgg atcgtccagc ccgcgctgga cgcgccgggc
      301 ccggtgcacc cctacgcccg cgggagctgg ggtcccgcga tcgccgatca gctcgtaggc
      361 tcggagggct ggtatgaacc gtcgatcggc accgaggctc gttcttcatg attcagttcc
      421 gttacgtcgc caccgaagag ttcgcatccg cgggtgcgga cctcatggcg acccacatca
      481 cccgggccat cgacgaccgc ggcgcctgcc acctcgcgtt gtccggcggc cgtgctccct
      541 gggagctgtt cggtgtcctc gcccgcaccg atctcccgtg gtccaaggtc cacgtctggc
      601 aggtcgacga gcgcgtcgcc ccggaccgtg acgtcgaccg caacagcgtc ggtctcaaga
      661 cgtcgctcct ggcgctcgct ccgatcctcg cgtccaacgt tcacctcatg gacgtcacgg
      721 cgcccgatct gggcgaggcc gctgccgcgt acgccgccga tctggcccgc ctgtgcgact
      781 ccacgctcga cctcgtccac ctcggactcg gcgccgacgg ccacacggcc agctggccac
      841 ccggcgaccc ggtgatcgac atcgacgacc gcgacgtcgc catcagcaag ctctacaacg
      901 gcttcgtccg catgaccctc accgtgccgt gcatcaaccg agcccgccac cgcgtgttcc
      961 tgatcaccgg cggcgacaag gagcacgcca tcaacgagct gggtgccatg ggcgacatcc
     1021 cggcatcacg ggtcagcgtc gacggtaccg acgcgctcgt ggcgctcccg atgccgctcc
     1081 tgcaagaact cgactgaccg gcggggtcgt gccggggcgg ctcagttgcg ctggatgtcg
     1141 acctcggtgc cgtcggccct ggcgatgagt cgaccgtttc cgcgcaggcc gttgacgccg
     1201 atgtagaccc ggggccgctc gcctgcgagg cggtcgaacc cgacgttgcc gacctcttct
     1261 ccctcgaggt cgagaccggt gagcgcctgg tcgatgaggc cggggatctt ctcccaggag
     1321 atgtcgccga ggtagaaggt gacactctcg tacgcctcga tctccgacac cgacaccgac
     1381 acgggtcgtg tcgtccacgt gccgttgtag aagtcgtagt cgtccatgtt gtcgcgcttg
     1441 gccggatccc ggacctggac actgaacccg ttggagttga gcgacaggcg ccggatctcg
     1501 gggctctccc ccaggtgctc gcggatgagg atcggcacct tttcggggtc ggccagcgcg
     1561 ttgacaccgc cgggagcgac cgtcgacgcc gtcacggcca ccgccgagtc gccgtcgcgg
     1621 ggctcgtcac cgcaaccggc gacaccggcc gtgacgacag cggccagcgt gatgccggcg
     1681 atcagccgcg ctcgtcggaa ccggcgtcgg tgagtttcgc gacgatgcat cggagggtgt
     1741 ccagttcggt ggtggagagc gccgcgaact ccggcggcgc cgtcgacagt cgggccatga
     1801 cactgtcacg gaggagccgg ccgtcggtcg tgaccgcgac ggcgcgcacc cggcgatcgt
     1861 ggggaaggct ctgtcgttcg acgatgcctc gttgttcgag gcggtcgacc atccaggtgg
     1921 cgttggacgg gtcgcaccgg aaggtctcgg cgaggacctt catgggcttc ggctcgtcgg
     1981 ggtcgagcga catcagcgcc tggaggtcgc cgggggtgag ttcgaattcg accgccacct
     2041 cgaccagcac ctccttacgg gcaaggaaga acgcgagcac cgacgaccat gcggcgtcgg
     2101 cgaggtcgga cttgttcctc ggggcgctca cgtgggccac tgtaccgacc gatgtgagat
     2161 gcatgagtct gggagaacgc gagaccgacg acgaggtcgc cgggcccgag ggtccaccgc
     2221 gttggcctgc cccgtcgagc cgacctgtcg gctcgcacca cgcgtacccg gcgcccgaca
     2281 gcgaggcgcc ggccgggtcc ggaacgtccg gcccgtcgac gaccgtcatc gcggtctgcc
     2341 tcggcctcgg gatctcgttg ttcgccctgg gcgttgccct catgacgatc gtcctctgac
     2401 gacgttcgac ggggcatcgg cctacgcttc ggctcgatga cggaactgat cggtgccctc
     2461 ggcgggttgg ggttggcctc gtcggcagga ctcaacgcct acatcccggc gttgctgacc
     2521 gcccttctcg cccggtacac cgatctggtc accttgccga aggagttcga gttcctgagc
     2581 tccggctggg tcctgatcac gctgaccgtg ctcctcgtga tcgaggaagt cgtcgacaag
     2641 gtccccggcg ccgaccacct caacgacgtg gtccagacgg tggtccgacc ggtgtcgggc
     2701 gccgtcctgt tcgccgccgg gtcggaggac gcgttcggca ggcacacctg gcttgcgctc
     2761 ggcgtggggt tcgtcgccgc gctgggagtc cacgcgacga aagcggccgg tcgcggcgtc
     2821 gtcaacgtgt cgacgggcgg catcggcgca ccggtcgtgt cggtgatcga ggacatcgtg
     2881 tcgctcaccg gcgccgtcgt cgcgatcgtc gctccggtcc tcgtgatcct gttcgtgatc
     2941 gcgttcgtct ggttgttggt cgtgatcctg cggaagcggc gcgaacgccg ggaacgcaag
     3001 gcggcggctg ccgttccgac ctgagtaccg ctcggctcgc agcccgagcc gagcaccaca
     3061 taccgtgtgc ccatgaccga gttcgcgttc tccgagatcc tccccgtcag cggcgactcc
     3121 accccgtacc gcctgctcac caccgagggt gtcagcacct tcgaggccaa cggcaaccgc
     3181 ttcctccagg tcgaacccga ggtgctcacg acactcacgg cgaccgccat gcgcgacatc
     3241 gcgcacctcc tgcgccccgg ccacctgcag cagctccgca acatcttgga cgaccccgag
     3301 gcgtcgtcga acgactgctt cgttgcgctc gagctgctga agaacgccaa catcgctgcc
     3361 ggcggtgtgc tcccctcctg ccaggacacg ggcacggcga tcatcaaggg caagaagggc
     3421 cagttcgtcg tcgtcaacgg cggccacgac gaggagcacc tcgcccgcgg cgtgttcgac
     3481 acctaccaga cgtccaacct gcgctacagc cagatggcgc cgctcacgat gtgggacgag
     3541 gtcaacacgg cgtcgaacct gcccgccgag atcaagatct ccgcgatcga cggcgacgcc
     3601 tacaagttca tgttcatggc gaagggcggc ggctcggcca acaagagcta cctcttccag
     3661 gagacgaagg cgctgctcaa cccgaagagc ctcatggcgt tcctggacaa gaacctccgc
     3721 tacatcggca cctccgcgtg cccgccgtac cacctcgcga tcgtcatcgg cggcacgtcg
     3781 gccgagttcg ccgtcgagac ggcgaagctc gcgtcgaccc gctacctcga cgacctgccc
     3841 aagcagggct cacccacggg ccacggcttc cgtgacgtcg aactcgaggc cgaggtgctg
     3901 gcgctcacgc agagcttcgg catcggcgcc cagttcggcg gcaagtactt ctgccacgac
     3961 gtgcgcgtga tccgccttcc ccggcacggg gcgtcgtgcc cggtcgcgat cgccgtgtcg
     4021 tgctcggccg accgccaggc gctggccaag atcacggccg acggtgtgtt cctcgagcag
     4081 ctcgaacacg acccggcccg gttccttccc gaggtgaacg aggctcacat cggcggcgag
     4141 gtgatctcga tcgacctgag ccggcccatg gacgagatcc gcgccgagct gacccgctac
     4201 cccgtgaaga cacggttgtc gctcaacggc acgctcgtcg tcgcccgcga tatcgcccac
     4261 gccaagatcg ccgagcgcct cgacgccggc gaggagatgc cctcgtacct tcgggaccac
     4321 gcggtctact acgccgggcc ggccaagacc ccgagcggta tggcgtcggg ctcgttcggt
     4381 ccgaccaccg ccggccgcat ggacagctac gtcgacaagt tccaggccgc cggcggctcg
     4441 atggtcatgc tggccaaggg caaccgcagc caggtcgtca cggacgcgtg caacacccac
     4501 ggcggcttct acctcggctc gatcggcggc cctgcggcga agctggcgca ggactgcatc
     4561 cgccacgtcg aggtgctcga ctaccccgag ctcggcatgg aggccgtgtg gaagatcgag
     4621 gtcgaggact tcccggcctt catcgtggtc gacgacaagg gcaacgactt ctacgcagac
     4681 gtctcgacgc cggtcgccct caggaagtag cgcaggcccc cagcccggcc tcagaaggtc
     4741 gtcgctccgt cgggcccgtc gaccgggtcg gtctcccatc cgaagtcgcc cgtggtctcg
     4801 ggcagctcgg cgacgtcgaa cacgaacggg tcggtgtcgg cgtcgatctt ctgcaccttg
     4861 ctcacgggtg cgcgggtgaa ggggttgcgg cgggtcagat cgatgacgtc gccctcgttg
     4921 gtgacgtcac cgacggcctc gacgactggg tcgtcgctgt cggcgggctc gacgacttcg
     4981 gcgtcggcga cctcggccgc ggtgtcgtcg gctgaggcgt ggtcgaccgt ggtgccatcg
     5041 gatcggccgt cggactcgtc gtcgccgacc ggctcggcgt cgttcgcggc cgacgcgatc
     5101 acgatcggct cggccgcaat ccccgtacgc aagcgcgtgc cgacggcatc gacggtgccc
     5161 ccgaggtcgg cgagaccgtc gtggtagcga ccgaagacgg cagtcagcgc gtcgagctgc
     5221 tcgaggatct cggagcgctg cgcctgccag gcggcgctcg ccgccaggat ctcgtcccgt
     5281 tcggcggtgt agtcctggtc cgcctgctgg cgcgcccggc ggatgatctc gctccgctgc
     5341 cgctcggcgt cggccacgat ggccaacgcc tcctcgcgcg cctcggcgat gaccgaatcg
     5401 gcggtctgct gagcgaaccg cagcgtgcgg gaggcactgt cgacctcggg atccccgctc
     5461 gtcgccacgg gggacacctg ggcggccacg agctgctcga ccatcgtctg caggctggcg
     5521 agccggtccc ggagatcgtc gtcggcggcg tcaccgttct tcgtcgccgt ggccgtcacc
     5581 gtggtcgtgg gcgtgtcggc gtcggaggac gtggccgcgg cggaacgctg ctcggcgtcg
     5641 gccaccttcg cggcgggctc ctcgttgggg cggaggaagt ccggggtcct gttgccccga
     5701 cgctccgggg tcgtcgtcgc gatttccgcg ttcgtctctc gtcgcctgcg tgccatgtcg
     5761 atcttctccc agcgccgggg gtcgctcccg cctgctgggc agggtaccga tgccggcccg
     5821 tgccgccaag acgggatcta cgacagcgcc gccgcgactc gacccccacc gaccggctcc
     5881 gccgcgtgcg ctgatgcatg gctcaactca ctgtcagggg tccgtgcttg actggtggca
     5941 tggtcacgaa caccagttcg cactccggcg gagggccgaa gagtcagctt gcggccgaca
     6001 tcatcgccgt ggggtcgacc ctgcgcgggt tggtgtcggg gctggaaccg aaggacgttc
     6061 cgttgccgtt cgccccggag gtgctggccg cgttcgcgga cgtgcagcgg gtggccgagt
     6121 cggggcgggt gttgatgacg gcgcgtgccg cccaagcgcg ggagtgggaa cgccaagggt
     6181 acgcgtccgc tgctgactgg ctcgcagccc agcagggcac cacgaccggg cgggcgcgtg
     6241 ccgacctcga aacgtccggg cacctcgaca acttggaagc aaccgcggac gcggtccgcg
     6301 ccggcaggct ctcgcccgag gcggcgggtg cggtggctga cgcggcggca gcgaaccctg
     6361 gagcggaaga cgacctgttg gacctggcgc agcgtgaatc gttgaagcgg acccgtcagg
     6421 aggcggagcg tcgcaaggcc gaggttcgat ccgaggacga gaaacgtcag cgcgaggagc
     6481 gggtgcggaa ggaccggagt ctgcggttct ggttcgggaa cggtgccggc aacctccacg
     6541 ccaccggccc gatcgacgtg ttgaaggaac tggaggcgtt gatccagcgt gaggtcgacc
     6601 ggaagttccg ggccaaccgt gacgcggcaa gtcgggagtc gcgggacaac tacgcgttcg
     6661 acgcgctgat cgacttggcc cgcaccggcg gcaccggggc caacaaggtt ggcgaaccgg
     6721 ccgaggaccg tgcggaatcg gcgggctcgg cgagttcggc agggcgggcc cggcgggcgc
     6781 cgttgacccg cctcacgttg atccgggtcg atctcgcagc gttgctgcgc gggagcgtcg
     6841 cccacgggga ggtgtgtgag atcggtggga tcgcggtatc ggttgctgaa ctccggcagt
     6901 tgttggggga gtcgatcatg cagctggtgc tcaccaacgg tgaagcggtc atcgacgtcg
     6961 tgaacctcgg tcgcaaaccg a
//
LOCUS       DAOSOA010000158         6973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10880_length_6973_cov_6.878867, whole genome shotgun sequence.
ACCESSION   DAOSOA010000158 DAOSOA010000000
VERSION     DAOSOA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10880_length_6973_cov_6.878867"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PKY13_10435"
     CDS             complement(<1..797)
                     /locus_tag="PKY13_10435"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOT80396.1"
                     /translation="MSLVVESVSHRNWEPWVFTANELDDLEAFFRVYGFGVLRGLWTE
                     EQMAELTTQCVTAQNGVIAGTLDERHGNTILVDDANSQIPFANYVQYLTEVAPVSRDM
                     ILDPMVVEVMERWLGPERWLLEHQRFGVVYQDARPGKESAYTRIGWHSDWQSAPHRDM
                     WPSVAFTIHIDGTSPANGFLRVVPGSHLWATPAPYRNANGVPVPDGSAPWGGYTDEPP
                     PFEMPLGFEKVPGEIATYCERGDVLFHDAYLWHSAARASAEGTMRRHV"
     gene            898..1920
                     /locus_tag="PKY13_10440"
     CDS             898..1920
                     /locus_tag="PKY13_10440"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOT80397.1"
                     /translation="MLLTNTTQELRHCWHVVATTDELGDEPLGVLLLGEPWVVVRLDG
                     IVRAFVDRCPHRLAPMSAGAVVPGEHGAELQCGYHGWRFDGDARCTAIPALSADDHIP
                     GRARLVPAFGVAERYGLIWIAPEEPLADLPTFDWYGADGYDSAMTELMRTPVSAAQLV
                     DNFLDAAHFPFVHTATFGVDEASEVHDRGITRDGWAVETIFDTWYRNGDDPLVATGEH
                     EAIQPQVLTKRGLASTTVVLTLGFPVTGATLAILFVCTPETATATRVYKMISRNDFGG
                     DAARMAGCVVEEDEILREDLSILERYHEMSLHLDPRVELHTKADRLSLAWRRMMAELL
                     AEVAER"
     gene            complement(1942..2988)
                     /locus_tag="PKY13_10445"
     CDS             complement(1942..2988)
                     /locus_tag="PKY13_10445"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOT80398.1"
                     /translation="MTVPTTNTVLTNDHPALLSQWHPIARVGADGGVGPAEDSVIAAE
                     LFARRYVVTVIGGVVTVAPDACPHRGVRLSRGEIVEGCLRCPYHGWRFGPTGECVAIP
                     AIGESPVPSRADLPTVPNEVALGLVWIRGDHGADGFDPIDVAEWGAVGIDAAWMSDVV
                     VGCSAGQFIDNFLDIAHFPVVHAGTFGADEATEVGDLAVESEPDDLGPGTLRLRHAHE
                     IVNREDPLVDTGEHPLVQPRVMEYTVKVPFSARLRIELPLTGVENTIVVVASPTGRER
                     TTLFTVLLRNDLRGDAEAARHAVDYEMAVLAEDLTILEHLADPGLPLDLPAQVHTRAD
                     KFTAAYRLALRRLL"
     gene            complement(2985..3593)
                     /locus_tag="PKY13_10450"
     CDS             complement(2985..3593)
                     /locus_tag="PKY13_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT80399.1"
                     /translation="MATFTDRYPVRIGSQSVELPLVPLHSSAAISLLMTIDRGVSFMA
                     TAGADLALELAALEPDIVATNATLGIPVAIEVTRALGLDDYLVLQKTPKIHLGDALVE
                     PLTSITTDSAQVLRLDRARLPAIDGKRVVLVDDVIASGGSIAAGCRLLRSGGANVVGI
                     GALLVEGSAWTEALGDDAALVRSLGSIPGFEPDGDGWAEVWE"
     gene            3707..5065
                     /locus_tag="PKY13_10455"
     CDS             3707..5065
                     /locus_tag="PKY13_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT80400.1"
                     /translation="MSTILIRNATVLPMSGTRQVFDPGSVLFDGTTIVAVGPVDEIDA
                     HPLAAGAEVVDATGHAVLPGLHNCHMHSGLLRGTAESMSLWDWLEAYVDPAHKAMTPE
                     IAKAASLVAYTENLRFGNTSVMDMWRFMEGSAEAAAAVGIRATLVPYVADEPGYDYFE
                     TLDSNLSLLKSHRTASDGRVRAWVGLEHLLYCSPTCFRDAMALAEEYDTGLHTHSSES
                     IWEVQQSLSRFGRRPIEEFFNRGILSERTVVAHCVWLDDREIELMAQTGTSAVHCPCS
                     NMKLASGPARVRDMRAAGIAFGLGSDGEKENNNLDLFEEMKFASLLQKVSTLDPTIGD
                     PWEVLTMATIEGAKALGLDDVTGSLEAGKRADIVMVDLGSIKFVPTFTAANGNGANFN
                     VAAHLVFTANGDDVDSVWVDGVRLVEGRTIRSVDEASVIATGQAAAEELFERRAAVLA
                     AQ"
     gene            5104..5844
                     /locus_tag="PKY13_10460"
     CDS             5104..5844
                     /locus_tag="PKY13_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT80401.1"
                     /translation="MGLANDRVDLLEHGEIGEEASPDESTFWDEMVEHIEQRRAATVD
                     PEEASFFNRPLSEHELVEWLSFQAYYELRACQFIGGWLATTPETDAMVLLAQQVEDEG
                     NHYEYCMRALARRGVTSLDNWTPEPEWEEWIDGWYPSGADTIERVAAHNLTGELGACQ
                     AFLQISPRLPDDVRKAFDRIIPDEQFHMKLGRKIIQKYCVTDDDRARVLARVDRTFEL
                     EQAGRLAYNRRMARLGIADADDAQLPMP"
     gene            5889..>6973
                     /locus_tag="PKY13_10465"
     CDS             5889..>6973
                     /locus_tag="PKY13_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HOT80402.1"
                     /translation="MSSPALNSLIVANPFRPAFRPSRRSPTMSTLVPVVDLSSPDAPA
                     AIDAACRESGFFQVVNHGLDLTLVEDLLAALETFFHLPADVKLQYPPPSPDVNNGYSA
                     IGAESLAYSLGVEAPPDLFEAFNFGPEDPDLTNPAVAAERDRIFAPNIWPAEVPELRT
                     AGVAYFDAAVALAHRITAACAVGLGLPGDFFEAFTGHSTDTMRCNWYSRVAGSPDPLP
                     GQQRMGAHTDYGIVTVLYADRVPGLELLDKRGEWIGVVPEPGAYLVNVGDLLAQWSND
                     RWVSTLHRVVPPPATTDGPSLRRSIAFFHDGDWDAMIQCLPTCCSEDEPPKYAPVRAL
                     DHLLNKLVGGRAMEPAETTAHIGDRIDA"
BASE COUNT         1138 a         2467 c         2346 g         1022 t
ORIGIN      
        1 acgtggcggc gcatggtgcc ctcggccgag gcccgcgccg ccgagtgcca caggtaggcg
       61 tcgtggaaga gcacgtcgcc tcgctcgcag tacgtggcga tctcaccggg caccttctcg
      121 aagccgagcg gcatctcgaa cggcggcggc tcgtcggtgt acccacccca gggcgccgac
      181 ccgtcgggga cgggcacgcc gttggcattg cggtagggag cgggggtcgc ccacaggtgg
      241 ctccccggca cgacccggag gaacccgttg gcggggctcg tcccgtcgat gtggatggtg
      301 aaggccaccg acggccacat gtcccggtgg ggcgccgact gccagtcgct gtgccacccg
      361 atgcgggtgt acgccgactc cttgcccggc cgggcgtcct ggtacacgac accgaagcgt
      421 tggtgctcga ggagccaacg ctcggggccg agccatcgtt ccatcacctc gacaaccatc
      481 ggatcgagga tcatgtcccg ggacaccggc gcgacctcgg tcaggtactg cacgtagttg
      541 gcgaacggga tctgactgtt ggcgtcatcg acgaggatcg tgttgccgtg acgctcgtcg
      601 agcgtcccgg cgatcacccc gttctgggcg gtcacgcact gcgtcgtgag ctcggccatc
      661 tgctcctcgg tccacagacc ccggagcaca ccgaacccgt acacccggaa gaaggcctcc
      721 aggtcgtcga gctcgttcgc ggtgaagacc cacggctccc agttgcgatg actcacggat
      781 tccacgacca gcgacatggc ccaaccctac gaacgcgccc ggcgcctccg acaggcacga
      841 tcgtggtctt cacgcgcacg aaacacaccg gcgacgtcgg gtggggacaa tgcctccatg
      901 ctcctcacca acaccaccca ggagctccga cactgctggc acgtcgtggc cacgaccgac
      961 gaactcgggg acgaaccgct cggcgtgctg ctcctcggcg agccgtgggt cgtggtccgt
     1021 ctcgacggga tcgtccgggc gttcgtcgac cgctgccctc accgcctcgc acccatgtct
     1081 gccggcgcgg tcgtgccagg cgagcacggc gcggagctgc agtgcggcta ccacgggtgg
     1141 cgcttcgacg gagacgcccg ctgtaccgcg atcccggcgc tgtccgccga cgaccacatc
     1201 cccgggcgcg cccgactcgt gccggcgttc ggtgtcgccg agcggtacgg cctgatctgg
     1261 atcgcgccgg aggaaccgct cgccgacctg ccgaccttcg actggtacgg cgcggacggg
     1321 tacgactcgg cgatgaccga gctgatgcgc accccggtgt cggcggcgca gctggtcgac
     1381 aacttcctcg acgcggcgca cttcccgttc gtgcacacgg ccaccttcgg ggtcgacgag
     1441 gcatcggagg tccacgaccg gggcatcacc cgcgacggct gggcggtcga gacgatcttc
     1501 gacacctggt accgcaacgg cgacgatccg ctcgtggcga ccggtgagca cgaggcgatc
     1561 cagccgcagg tgctcaccaa acgtgggctc gcgtcgacga cggtcgtgtt gaccctcggc
     1621 ttccccgtga ccggtgcgac gttggccatc ctcttcgtgt gcacgccgga gacggcgacg
     1681 gcgacgcggg tgtacaagat gatctcgcgc aacgacttcg gtggtgatgc ggcgcgcatg
     1741 gccgggtgtg tggtcgagga ggacgagatc ctccgcgagg acctgtcgat cctcgagcgc
     1801 taccacgaga tgtcgctgca cctcgacccc cgggtcgagc tgcacacgaa agcggatcgt
     1861 ctcagcctgg cgtggcggcg aatgatggcg gagctgctcg ccgaggtagc ggagcggtag
     1921 tcgggagcgc ggcgatcgaa cctagaggag ccggcgaagg gccagccggt acgcggcggt
     1981 aaatttgtcg gcccgcgtgt ggacctgcgc cggaaggtcg agtgggagac cggggtcggc
     2041 gagatgctcg aggatggtca ggtcctcggc gagcaccgcc atctcgtagt cgaccgcgtg
     2101 gcgggcggcc tcggcgtctc cccggaggtc gttgcggagc aacaccgtga acaacgtggt
     2161 ccgctcgcgc ccggtgggtg aggccacgac gacgatcgtg ttctcgacgc cggtcaacgg
     2221 cagctcgatg cggagccggg ccgagaacgg caccttcacc gtgtactcca tcacccgcgg
     2281 ctgcacgagg ggatgctcac cggtgtcgac gagcgggtcc tcgcggttca cgatctcgtg
     2341 ggcgtgccgg agccgaagcg tccccggccc gaggtcgtcg ggttcggact cgacggcgag
     2401 gtcgcccacc tcggtcgcct cgtccgcacc gaacgtgccg gcgtggacca ccgggaagtg
     2461 ggcgatgtcg aggaagttgt cgatgaactg cccggccgaa caccccacga cgacgtcgga
     2521 catccaggcc gcgtcgatgc cgaccgcacc ccactccgca acgtcgatcg gatcgaaacc
     2581 gtcggcgccg tggtcgccgc ggatccacac caggccgagc gcgacctcgt tcggaacggt
     2641 cggcaggtcg gccctcgacg gcacgggcga ttcgccgatg gcggggatgg cgacgcactc
     2701 gcccgtcggg ccgaatcgcc atccgtggta cgggcagcga aggcacccct cgacgatctc
     2761 gccgcgtgac agtcgcacac cacggtgtgg gcaggcgtcg ggggccaccg tgacgacacc
     2821 gccgatcacg gtcaccacgt accggcgggc gaacagctcg gcagcgatga cgctgtcctc
     2881 ggcaggtcct acaccgccgt cggcaccgac ccgggcgatc gggtgccact gcgacaacaa
     2941 cgcggggtga tcgttggtca ggacggtgtt cgtggtcggc acggtcactc ccacacctcc
     3001 gcccagccgt cgccgtcggg ctcgaacccc ggaatcgatc cgagcgaacg gacgagcgca
     3061 gcgtcgtcgc ccagggcctc cgtccacgcg cttccctcga ccaggagtgc gccgatgccg
     3121 acgacgttcg cgccgccgga gcgcagcagc cggcaccccg cagcgatcga cccaccgctc
     3181 gcgatgacgt cgtcgaccaa cacgacccgc ttgccgtcga tggccggcag gcgcgcccgg
     3241 tcgaggcgga gcacctgagc gctgtcggtc gtgatcgacg tcagcggctc gacgagggcg
     3301 tcaccgaggt ggatcttggg cgtcttctgc aacacgaggt agtcgtcgag cccgagtgct
     3361 cgggtcacct cgatcgcaac cggaatgccg agcgtggcgt tcgtggccac gatgtcgggc
     3421 tcgagcgcgg cgagctcgag cgccagatcg gcgccggccg tggccatgaa cgagacccca
     3481 cgatcgatcg tcatcagcag cgagatcgcg gcggacgagt gcagggggac aaggggcagc
     3541 tcgacggatt gcgagccgat ccggacgggg tagcggtcgg tgaacgttgc catcgggcga
     3601 aggtaacgaa gccgatcgcg ccgttgtcga ccgctccgtg aatggcgtgt gaacaggtgc
     3661 ccgagtgcct tgaccatcgg gcggtcaacc gggagattgg cctcagatgt ccacgatcct
     3721 gatccgcaat gcgaccgttc tcccgatgtc cggcacccgc caggtgttcg atccgggctc
     3781 cgtgctgttc gacggcacga ccatcgtggc cgtcggcccc gtcgacgaga tcgacgccca
     3841 tccgctcgcc gccggcgccg aggtcgtcga cgccaccggc cacgccgtgc tgcccggcct
     3901 ccacaactgc cacatgcact ccgggctgct gcggggaacc gccgagtcga tgtcactgtg
     3961 ggactggctc gaggcctacg tcgatccggc ccacaaggcg atgacccccg agatcgccaa
     4021 ggcggcgagc ctcgtcgcct acaccgagaa cctgcggttc gggaacacct cggtcatgga
     4081 catgtggcgc ttcatggaag ggtcggccga ggccgctgcc gccgtcggca tccgggcgac
     4141 gctcgtgccc tacgtcgccg acgagccggg ctacgactac ttcgaaaccc tcgactccaa
     4201 cctgtcgctc ctcaagagcc accgcacggc gtcggacggc cgggtgcgtg cgtgggtcgg
     4261 gctcgaacac ctcctctact gctcccccac gtgctttcgc gacgccatgg cgctcgctga
     4321 ggagtacgac accgggctgc acacccacag ctcggagtcg atctgggagg tccagcagtc
     4381 gttgtctcga ttcggccggc ggccgatcga ggagttcttc aaccgcggca tcctgtcgga
     4441 gcgcacggtc gtcgcgcact gcgtctggct cgacgatcgc gagatcgagc tgatggccca
     4501 gacggggacg tcggccgtgc actgcccgtg ctcgaacatg aagctggcat cgggtccggc
     4561 ccgggtgcga gacatgcgcg ccgccggcat cgcgttcggc ctcggcagcg acggcgagaa
     4621 ggagaacaac aacctcgacc tcttcgagga gatgaagttc gcgtcgctgc tgcagaaggt
     4681 gtcgacgctc gaccccacca tcggcgaccc gtgggaggtc ctcacgatgg ccaccatcga
     4741 gggcgccaag gccctcggcc tcgacgacgt gaccggctcg ctcgaagcag gcaagcgggc
     4801 cgacatcgtg atggtcgacc tcggctccat caagttcgtc ccgaccttca ccgcggccaa
     4861 cggcaatggc gccaacttca acgtggccgc acacctcgtg ttcaccgcca acggcgacga
     4921 cgtcgactcg gtgtgggtcg acggcgtgcg actcgtcgag ggccgcacga tccgaagcgt
     4981 cgacgaggcg tcggtcatcg cgaccggcca ggccgccgcc gaggaactgt tcgaacgacg
     5041 ggctgccgtc ctcgcagcgc aatgacatga accacgagac cgacaccgag agacaggact
     5101 gacatgggat tggccaacga ccgggtggac ctcctggagc acggcgagat cggcgaggag
     5161 gcatcaccgg acgaatcgac gttctgggac gagatggtcg agcacatcga gcagcggcgg
     5221 gcggccaccg tcgaccccga ggaggcgtcg ttcttcaatc gaccgctgtc ggagcacgag
     5281 ctggtcgagt ggctgtcgtt ccaggcgtac tacgagctgc gggcctgcca gttcatcggc
     5341 gggtggctgg cgaccacccc cgagaccgac gccatggtgc tgctcgcaca gcaggtcgag
     5401 gacgagggca accactacga gtactgcatg cgcgccctcg ctcgacgcgg cgtcacgtcg
     5461 ctcgacaact ggacgccgga gcccgagtgg gaagagtgga tcgacggctg gtacccgtcg
     5521 ggcgccgaca cgatcgaacg ggtcgccgcc cacaacctca ccggcgaact cggcgcgtgc
     5581 caagcgttcc tgcagatcag cccacgtctc cccgacgacg tgcgcaaggc cttcgaccgg
     5641 atcatccccg acgagcagtt ccacatgaag ctcggccgca agatcatcca gaagtactgc
     5701 gtcaccgacg acgaccgcgc ccgcgtgctc gcccgcgtcg accgcacctt cgagctcgag
     5761 caggccggac gcctcgccta caaccgccgc atggcccggc tcgggatcgc cgacgccgac
     5821 gacgcccagt tgccgatgcc gtgagccgga ccaccacctg aaccaccgac cggaggaacc
     5881 tctcgccgat gagcagccca gcactcaaca gcctgatcgt cgccaacccg ttccgccccg
     5941 cgtttcgacc ctcccggagg tcacccacga tgtccaccct cgttcccgtc gtcgacctgt
     6001 cgtcgcccga cgctcccgcc gccatcgacg ccgcctgccg tgagagcggt ttcttccagg
     6061 tggtcaacca cggcctcgac ctgacgctgg tggaggacct cctcgcggcg ctcgagacgt
     6121 tcttccacct gccggccgac gtgaagctgc agtacccgcc gccgtcaccc gacgtgaaca
     6181 acggctactc ggcgatcggc gcggagtcgc tggcgtacag cctcggagtc gaggcaccgc
     6241 ccgacctgtt cgaggccttc aacttcgggc cggaggatcc cgacctcacc aacccggcgg
     6301 tcgccgccga acgcgaccgc atcttcgccc ccaacatctg gccggccgag gtgcccgagc
     6361 tgcgcaccgc gggcgtggcg tacttcgacg ccgccgtcgc cctggcccac cgcatcacgg
     6421 cggcctgcgc ggtggggctc ggcctgccgg gcgacttctt cgaggcgttc acgggccact
     6481 cgaccgacac gatgcggtgc aactggtact cccgggtggc cggctcgccc gatcccctcc
     6541 ccgggcagca gcgcatgggc gcgcacaccg actacggcat cgtcacggtg ctgtacgcgg
     6601 accgtgtccc cggcctcgaa ctcctcgaca agcgtggtga gtggatcggg gtcgtgcccg
     6661 aaccgggcgc gtacctcgtg aacgtgggtg acctgttggc gcaatggtcg aacgaccggt
     6721 gggtgtcgac gttgcaccgg gtcgtgccac cgccggcgac gacggacggc ccgtcgctgc
     6781 gccgctcgat cgcgttcttc cacgacggcg attgggacgc catgatccag tgcctcccga
     6841 cctgctgctc ggaggacgaa ccgccgaagt acgcgccggt ccgggccctc gaccacctcc
     6901 tgaacaaact cgtcggcggc cgggccatgg aaccggcgga gaccaccgcc cacatcggcg
     6961 accgaatcga cgc
//
LOCUS       DAOSOA010000159         6973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10881_length_6973_cov_6.693842, whole genome shotgun sequence.
ACCESSION   DAOSOA010000159 DAOSOA010000000
VERSION     DAOSOA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10881_length_6973_cov_6.693842"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..666)
                     /locus_tag="PKY13_10470"
     CDS             complement(4..666)
                     /locus_tag="PKY13_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT80403.1"
                     /translation="MTEPTEPPTTASALGPVRLVVADDHPIWRSGIRADLGDNFVIVG
                     EAGDATEAIEVIRTTTPDLVLSDLHMPGGGGVAVVRACGATTRIVILTVSEAERDLLD
                     AVAAGAVGYLVKSTPMEELRQQLWRAAHGEPVFSPSLAALVLGEFRRLSRDTNPGAPL
                     SDREREVLQLVARGHKYKEISASLFIAEKTVENHVRNILGKLHLNRREELIRFAVEHG
                     LD"
     gene            complement(663..1784)
                     /locus_tag="PKY13_10475"
     CDS             complement(663..1784)
                     /locus_tag="PKY13_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT80404.1"
                     /translation="MPPPLVDPTDRAGLDQGLEVGVILFRWTAWIWMVVVAVVERDHL
                     SHPLPAALLIGAAFAVTASASVMRAVVPGSSIRPATVVVELAVGAGLLVGSAVFQDGD
                     QSQSLGSAWPLVGPLTAGLVFGPRIGIASGLALGVARGASGFFGGPGVSAVAVVSSAV
                     LYGLAGGVCGWVAARLRRSAANAALLADMEASLAVAREREDVARTLHDGVLQTLAVVQ
                     RRSTDPELATLARRQELDLRTYLFGVEPAVDLLASLRDVAARHEQSLGTRVTVVCPDE
                     LGHVGSELIDAMRGAVTEALTNAAKHGRATNVTVFVERDDDGIFVSVKDDGVGFDPES
                     TDDGVGLARSIRARMREIGGRVEVDSRPGSGAEVRLWGR"
     gene            complement(1829..4438)
                     /locus_tag="PKY13_10480"
     CDS             complement(1829..4438)
                     /locus_tag="PKY13_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT80405.1"
                     /translation="MIRVALRGLRGNARRLVGTFIAIVLGVSFMSGVRILGDTTRASF
                     DSLFATIGNGIDVVVRVTPRDDGRDGQEGGDNSGSAAFDLRTVPAALATEVEAVDGVA
                     AVQTVHETRRGVVVVGRDGQPVSLGPGISFLASEYPTVPAMDPYTLTRGGRAPQADDE
                     VVLDRATAEAGELSVGDTVTIRTPTSVATYRLVGTVSLGETSGGGAGRALFTTAVATS
                     FMTDPAEASRLLLLADPGVSPDVLRDRVGAVVAADVTPTRKLKTITGEEYVADLRGRI
                     GEFLDILQNGLTGFAGLSLFVGAFVIYNTFSILVAQRQRELALLRAVGAARRQVVGAV
                     LVEAAAVGAVAGIAGVAGGALLSRGLSAGFTAFGFDLPSSTIVVTFAVAVTSFVIGLT
                     MALGCALFPALRAARIPPIAALRDIAVDRSDRSRGRLVVGSLFVVASAALLVRGLSVT
                     EEGSTAAVTVGVATAVAFVAVAVLGPLISAPIARLVGAPLLAAGTTGRLARANATRNP
                     RRTASTAAALMIGVSIVTFVLVMSATLESVVAETTERSLTADVIVSGDAFGRGAIAGD
                     AAAEIAAVSGVVKVGTVTSLRAKYFDDLQSLAAVSPDALSQAVLTVTDGSLDGLGLDG
                     VAVEQDEADEFGLRVGDRIPVAFVDGAIATPRIVAVLDNLDVIGSNLLTSSSFAEQYQ
                     PEIGPSQILVETSTNTSTARIIRDIKAIPILIGTQVQDLSTYADAQTSALDSILGIFG
                     VMLALTVLIALFGIANTLSLSIHERTREIGMLRAVGTTRRQVGRMVRMESVILALLGT
                     FEGAVIGLLFGWAIADTLVSDPTAPPGLAVVLPLGQVAGVVLIAIVAGVVAAATSARR
                     ARRLDVLAAISSE"
     gene            complement(4435..5136)
                     /locus_tag="PKY13_10485"
     CDS             complement(4435..5136)
                     /locus_tag="PKY13_10485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOT80406.1"
                     /translation="MRTLATADGPMDLIVAEPAGAPKGGVVVVQEAFGLTPHLHNICA
                     RLAEAGWLAVAPALYHRTGSPVLGYDEFDQVAPHIMAIDPDGLATDLGAALEVLDDAG
                     IDPDRRGVIGFCMGGTIALWAATVFELGAAVTFYGSGIASNRFGLPPLVELAPALRAP
                     WQGHYGDEDTGIPVEQVEDLRAAAGHSAVEFELHRYPDAPHGFNCDERGSYHEPSARL
                     AWQRSLDWFDRHLAG"
     gene            5244..6434
                     /gene="dinB"
                     /locus_tag="PKY13_10490"
     CDS             5244..6434
                     /gene="dinB"
                     /locus_tag="PKY13_10490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT80407.1"
                     /translation="MSRTAAILHADLDAFFASVEQRDDPGLRGRPVIVGGGVVLAASY
                     EARAYGVRGAMGGSQARALCPQVVVVQPRFSAYVEASRAVFSVFERTTPFVEGISIDE
                     AFLDVGGLRRVAGTPVEIAVRLRRQVREEVGLAITVGVASTKFLAKVASAVGKPDGLL
                     VVPNGGELDFLHPLPVGRLWGVGPKTAEKLHERGITTVAQVARIPESALVAMLGGHAG
                     RHLHALAHNRDHRSVQTGRRRRSIGSQRALGRSNPSLGELDTTLVGLVDRVTRRLRDA
                     DRLARTVVLRLRFSDFTRATRSHTLGESTSHTDTILEALRALLADAGPTIAERGITLL
                     GVALANLDDGDVTQLELPFGRRSGNALDDVLDDVRERFGGDALGRAVMLGRDQGWSMP
                     TLPE"
     gene            6550..6927
                     /locus_tag="PKY13_10495"
     CDS             6550..6927
                     /locus_tag="PKY13_10495"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT80408.1"
                     /translation="MSADPLSKVFSALADPIRRDLVARLADGDATVNELAAPYDVSLQ
                     AVSKHLKVLEDAGLVTKGRQAQRRPVHLEAEVFDLMTKWIERYRRQAEARYRRLDDIL
                     ATMPDDVRPEPPPKRQNPTGGPS"
BASE COUNT         1059 a         2394 c         2569 g          951 t
ORIGIN      
        1 cgttcagtcg agaccgtgct cgacggcgaa gcggatcagc tcctcccgcc ggttgaggtg
       61 cagcttgccg aggatgttcc gtacatggtt ctcgacggtc ttctcggcga tgaacaggct
      121 cgccgagatc tccttgtact tgtggccgcg ggccacgagc tggaggacct cccgctcccg
      181 gtcgctgagc ggcgcgccag ggttggtatc gcgcgacaga cggcggaact cgccgagcac
      241 gagtgcggcg agcgacggcg agaacaccgg ttccccgtgg gcggcgcgcc agagttgctg
      301 ccgcaactcc tccatgggcg tcgacttcac gaggtacccg acggcgccgg cggcgacggc
      361 gtcgaggagg tcgcgctcgg cctccgacac ggtgaggatg acgatgcgcg tcgtcgcgcc
      421 gcaggcgcgg accacggcga ctccgccacc gccgggcatg tgcagatcgc tgagcacgag
      481 atcgggggtg gtggtccgga tgacctcgat ggcctcggtc gcgtcgcctg cctcgccgac
      541 gatgacgaag ttgtcgccga ggtccgcccg gattcccgat cgccagatcg gatggtcgtc
      601 ggcgacgacg agccgaaccg ggccgagcgc gctggcagtg gtgggtggct ccgttggctc
      661 ggtcatcggc cccacaatcg cacttcggcg cccgagcccg ggcgcgaatc gacctcgacc
      721 cgacctccga tctcgcgcat ccgggcgcgg atggaccggg ccagaccgac gccgtcgtcg
      781 gtgctctcgg ggtcgaagcc gaccccgtcg tccttcaccg acacgaagat cccgtcgtcg
      841 tcccgctcca cgaagaccgt cacgttcgtg gcccgtccgt gcttcgcagc gttggtcagc
      901 gcctcggtga ctgcaccgcg catggcgtcg atcagctcgg acccgacgtg gcccagctcg
      961 tcggggcaga cgacggtgac ccgggtgccg agtgactgct cgtggcgggc ggcgacgtcg
     1021 cgcagcgatg ccagcaggtc gaccgccggt tcgacgccga agaggtacgt ccgcaggtcg
     1081 agctcctggc gtcgggccag ggtcgcgagt tcggggtcgg tcgagcgtcg ctggaccacg
     1141 gcgagcgtct ggagcacgcc gtcgtgcagg gtgcgggcga cgtcctcacg ctcgcgggcg
     1201 acggcgaggc tcgcctccat gtcggcgagg agcgctgcgt tggcggctga ccggcgaaga
     1261 cgggccgcga cccagccgca cactccgccg gcgagcccgt acaggacggc cgatgacacc
     1321 acggccacgg ccgacacccc gggaccgccg aagaagccgg acgcgccgcg ggcgacgccg
     1381 agggcgaggc ccgacgcgat cccgatccgc ggtccgaaca ccaagccggc ggtgagcggt
     1441 ccgacgagcg gccacgccga cccgagcgac tggctctggt cgccgtcctg gaacacggcg
     1501 ctgcccacga gcaggccggc gccgactgcc agctcgacga cgacggtggc gggacggatc
     1561 gatgagcccg gaaccacggc ccgcatcacc gatgccgagg cggtgacggc gaacgccgcg
     1621 ccgatgagca gcgcggcggg gagcggatgg ctcaggtggt cgcgttccac gaccgccacg
     1681 acgaccatcc agatccacgc cgtccaccgg aacaggatca cgccgacctc gagaccctgg
     1741 tcgagcccgg cgcggtccgt cggatcgacg agcggtggtg gcacgtcgtc gagtctacgg
     1801 atcgaccccg gcggtccggc ggcgaccgtc actccgacga gatcgctgcc aggacgtcga
     1861 ggcgccgcgc ccgtcgagcc gaggtcgcgg cggcgacgac accggcgacg atggcgatga
     1921 gcacgacccc ggcgacctgg ccgagcggca gcaccaccgc gaggccagga ggcgccgtgg
     1981 gatcgctgac gagcgtgtcg gcgatggccc agccgaacag caggccgatc accgcgccct
     2041 cgaacgtgcc gagcagcgcc aggatgaccg actccatccg caccatgcgc ccgacctgac
     2101 ggcgggtggt gccgacggcg cgcagcatgc cgatctcgcg ggtccgctcg tggatcgaca
     2161 ggctcagcgt gttggcgatc ccgaacagcg cgatgagcac cgtgagggcg agcatgaccc
     2221 cgaagatccc gaggatgctg tcgagggccg acgtctgcgc gtcggcgtag gtcgagaggt
     2281 cctggacctg ggtgccgatc aggatcggga tggccttgat gtcccggatg atcctcgccg
     2341 tcgacgtgtt cgtcgacgtc tcgacgagga tctgactcgg gccgatctcc ggctggtact
     2401 gctcggcgaa cgaactcgag gtgagcaggt tcgatccgat cacgtcgagg ttgtcgagca
     2461 cggcgacgat ccggggagtg gcgatcgctc cgtcgacgaa cgcgacgggg atgcggtcgc
     2521 cgacccggag tccgaactcg tcggcctcgt cctgctcgac agcgaccccg tcgagcccga
     2581 ggccgtcgag cgaaccatcg gtcaccgtga gcacggcctg ggacaacgcg tcgggactga
     2641 cggcggcgag gctctggagg tcgtcgaagt acttggccct caggctcgtc acggtgccga
     2701 ccttcaccac cccggacacg gcggcgatct cggctgcggc gtcgccggcg atggcgcccc
     2761 ggccgaacgc gtcgccgctg acgatgacgt cggcggtgag cgatcgttcc gtcgtctcgg
     2821 ccacgacgga ctcgagcgtt gccgacatga cgagcacgaa cgtcacgatc gacacgccga
     2881 tcatcaacgc cgcggccgtg gacgcggtgc ggcgggggtt gcgcgtcgcg ttggcccggg
     2941 cgagccggcc ggtcgtcccg gcggcgagca gcggcgcgcc gaccagccgg gcgatcggtg
     3001 ccgagatgag cggtccgagc accgccactg cgacgaacgc gacggccgtc gccaccccga
     3061 cggtgacggc tgcggtcgac ccctcctcgg tcacgctcag gccgcggacg agcagcgcgg
     3121 ccgacgcgac cacgaacagc gacccgacga cgaggcgtcc tcgggaccgg tcggaccggt
     3181 cgacggcgat gtcgcgcaac gctgcgatcg gcgggatccg ggcggcgcgg agggccggga
     3241 acagcgcgca gccgagcgcc atcgtgagcc cgatgacgaa cgaggtgacg gccacggcga
     3301 acgtgacgac gatcgtgctg gaggggaggt cgaacccgaa cgccgtgaac ccggcggaca
     3361 gcccccgcga cagcagcgcg ccgccggcca cgccggcgat gccggcgacg gccccgaccg
     3421 cggctgcctc gacgaggacc gccccgacca cctgtcgccg tgccgcgccg actgcccgga
     3481 gcagggcgag ctcacgctgt cgttgggcga cgaggatgga gaacgtgttg tagatcacga
     3541 acgccccgac gaacagcgac aggccggcga acccggtcag cccgttctgc aggatgtcga
     3601 ggaactcgcc gatccggccc cgcaggtcgg cgacgtactc ctcgccggtg atggtcttga
     3661 gtttgcgggt gggggtgacg tcggcggcca cgacggcgcc gacgcggtca cggaggacgt
     3721 cgggcgagac gcccggatcg gcgagtagga ggagccggct cgcttctgcg gggtcggtca
     3781 tgaacgaggt cgcgaccgcc gtggtgaaca acgcccgacc ggcaccaccg cccgaggtct
     3841 cgccgaggga cacggtgccg acgaggcggt aggtggcgac cgacgtcggg gtgcggatcg
     3901 tgacggtgtc gccgacgctg agctcgccgg cctcggcggt cgctcgatcg agcacgacct
     3961 cgtcgtcggc ctgcggtgcg cggccgcccc gggtgagggt gtaggggtcc atcgccggca
     4021 cggtggggta ctccgaggcc aggaacgaga taccgggccc gagggacacg ggttgaccgt
     4081 cgcgcccgac gaccacgacg ccgcgacgcg tctcgtgcac cgtctgcacc gcggcgaccc
     4141 cgtcgacggc ctcgacctcg gtcgcgagcg ccgccggcac ggtgcgcagg tcgaaggccg
     4201 cgctcccgct gttgtcgccg ccctcttgac cgtcgcgccc gtcgtcgcgg ggcgtgaccc
     4261 ggaccacgac gtcgatgcca ttgccgatgg tcgcgaacaa cgagtcgaac gaggccctgg
     4321 tcgtgtcgcc gaggatcctc acgccggaca tgaacgacac acccagcacg atcgcgatga
     4381 acgtgccgac gaggcggcgg gcgttgccgc gcaggccgcg gagtgcgacg cgaatcaccc
     4441 ggcgaggtgt cggtcgaacc agtcgagcga ccgctgccac gcgagccgcg ccgagggttc
     4501 gtggtacgag ccgcgttcgt cacagttgaa gccgtgcggg gcgtcgggat agcggtgcag
     4561 ctcgaactcg acggcgctgt ggccggccgc ggcgcggagg tcttccacct gctcgacggg
     4621 gatgccggtg tcctcgtcgc cgtagtgccc ctgccacggt gctcgcagcg ccggagccaa
     4681 ctcgacgaga ggtggcagcc cgaagcggtt cgaggcgatc cccgatccgt agaaggtgac
     4741 ggctgcgccc agctcgaaca cggttgcagc ccacagcgcg atggtgccgc ccatgcagaa
     4801 gccgatgacc ccgcgacggt ccgggtcgat accggcgtcg tcgagcacct cgagcgcggc
     4861 gccgaggtcg gtcgccagcc cgtcgggatc gatggccatg atgtgcggtg ccacctggtc
     4921 gaactcgtcg tagccgagca ccggtgagcc ggtgcggtgg tagagcgccg gggcgaccgc
     4981 caaccatccg gcctcggcga ggcgagcgca gatgttgtgg aggtgggggg tgaggccgaa
     5041 cgcttcctgg acgacaacga cgccgccctt gggtgcgccg gccggttcgg cgacgatcag
     5101 gtccatcggg ccgtcggcag tggcgagggt acgcatcggg gcagcctggc acgtcaccgg
     5161 cgaggggtgc aggcacccgg gaccgtgccg acggtgcggc gaagggcttg ccgcctccct
     5221 ggtcgtgggc gaacatatgt tcgatgtctc gaactgcggc catcctgcat gccgatctcg
     5281 acgcgttctt cgcgtcggtg gagcagcgcg acgacccggg gttgcggggc cgacccgtga
     5341 tcgtcggggg cggggtggtg ttggcggcca gctacgaggc ccgggcgtac ggcgtgcgtg
     5401 gtgcgatggg cggctcgcag gcgagggcgc tgtgtccgca ggtggtggtc gtccaaccgc
     5461 gtttctcggc gtacgtcgag gcgagccggg ccgtgttctc cgtcttcgag cggacgacgc
     5521 ccttcgtcga gggcatttcg atcgacgagg ccttcctcga cgtgggtggg ctccggcggg
     5581 tggcgggcac gccggtcgag atcgcagtcc gtctgcgccg gcaggtgcgg gaagaggtcg
     5641 ggctcgcgat cacggtgggc gtcgcctcca cgaagttcct ggccaaggtg gcgagcgccg
     5701 tcggtaagcc cgacgggctc ctcgtggtgc cgaacggggg tgagctcgac ttccttcacc
     5761 cgctccccgt cgggcgcctg tggggtgtcg gtcccaagac ggcggagaag ctgcacgagc
     5821 gggggatcac caccgtggcc caggtggcgc gcatcccgga atcggcgctg gtcgccatgc
     5881 tcggtggtca cgccgggcgg catctccacg cgctcgccca caaccgggac caccggtcgg
     5941 ttcagacggg ccgtcggcgg cggtcgatcg gctcgcagcg tgccctcggt cgctccaatc
     6001 cttcgctcgg cgagctcgac accacgctcg ttggtttggt cgatcgggtg acccgccgtc
     6061 tgcgtgacgc cgaccggctg gcccggaccg tggtgcttcg gctccggttc tccgacttca
     6121 cgcgggcgac gcgctcgcac accctgggcg agtcgacgtc gcacaccgac acgatcctcg
     6181 aggcgctccg ggccctgctg gcggacgccg ggccgaccat cgcggagcgg gggatcacgt
     6241 tgctcggggt cgcgctggcc aatctcgacg acggcgacgt cacgcagctg gagcttccct
     6301 tcggacggcg gagcggcaac gcgctcgacg acgtcctcga cgacgtgcgg gaacggttcg
     6361 gtggtgacgc cctcggacgg gcggtgatgc tgggccgcga tcagggctgg agcatgccca
     6421 cgctgcccga gtgaacgggt gtggtgcgcc gggacgacgt cgacgaccga atgttcaacc
     6481 aagtggttga caatgtccgg cggcccgtcg taccttgttc aaccaagagg ttgaacaagg
     6541 agctcgtcga tgtcggcaga cccactctcg aaggtgttct cggccctcgc cgacccgatc
     6601 cgccgcgacc tggtggcgcg gttggccgac ggcgatgcca ccgtcaacga gctggcagcg
     6661 ccgtacgacg tctcgctcca agccgtgtcc aagcacctca aggtgctcga ggatgcgggc
     6721 ctcgtcacca aggggcggca ggcgcaacgc cggcccgtcc acctcgaggc ggaggtcttc
     6781 gatctcatga ccaaatggat cgagcgctac cggcgccagg ccgaagcgcg gtaccgccgc
     6841 ctcgacgaca tcctggcgac catgcccgac gacgttcgac ccgaaccacc acccaagcgc
     6901 cagaacccga ccggaggacc atcatgacca ccaacccgaa cgagaccgag atcttcgtcg
     6961 atcccgacgt gcc
//
LOCUS       DAOSOA010000160         6951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10938_length_6951_cov_4.912848, whole genome shotgun sequence.
ACCESSION   DAOSOA010000160 DAOSOA010000000
VERSION     DAOSOA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10938_length_6951_cov_4.912848"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKY13_10500"
     CDS             complement(<1..749)
                     /locus_tag="PKY13_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOT80409.1"
                     /translation="MTQTSPSRRSSPASPAGRPRDGLLDLLRSLSITRVIGIHLIGRS
                     GFWFWPAPTYVLPGMPMVFFVSGALVRKTLGTVDGARRSALTYWRSTFRRLLVPYWAY
                     YAVMVLVAVVADAGHTSDYWTVHHDDLLLGATGFVVPEGSLFMRRHTGHLWFMSAFLV
                     LTLFAPLLVRLFERFRGLVMILPLSLFAWVEWRISGGEDVRAELDNPPLADLLALDDA
                     AFRARFRGTPVKRTGRDRFVRNVLIAIGNSG"
     gene            922..1455
                     /locus_tag="PKY13_10505"
     CDS             922..1455
                     /locus_tag="PKY13_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT80410.1"
                     /translation="MSQVTESPPIPDAPEPDAGAAPAAELDCSIARALGVIGDRWTIL
                     IMRDAFRGIRRFDDFRRDLDIARPVLADRLKKLVDAGIMVRRRYQTSPERFEYRLTPM
                     GMELSPALVALMRWGDRWLNDGRSPTVLVHEPCGSELEQGFWCRTCQQTFTPGDIASR
                     NPHSATPHSSGATHVHH"
     gene            1442..3832
                     /locus_tag="PKY13_10510"
     CDS             1442..3832
                     /locus_tag="PKY13_10510"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HOT80411.1"
                     /translation="MSTTDRSLTDVPLAELLAEAAARRDAHHGNRVTYSPKVFIPLTM
                     LCQDKCGYCTFAQPPARLDAPYLTPEQVLAIAAAGAEVGCHEALFTLGERPELRYPVA
                     AEWLAEHGYASTVDYLAAMCALVRDETGLLPHANAGALTRDELAQLREVSASQGMMLE
                     SLNPNLDAHRGSPDKTPERRLATLEWAGELQIPFTTGILVGIGESRSDRVDALEAIAE
                     SHRRHGHVQEVIVQNFLPKPGTSMHAARPCPTDDYLDAIALARLILPADVHLQVPPNL
                     TDDFGSLLGAGIDDWGGVSPVTADHVNPERPWPAIDRLRAVTEAAGHTLAPRLTIYPE
                     FAAAPERWLAGPMRFPVLDRSDAESLARDRDTLDEHPGATVWYSGADTDPPVVIETSS
                     VTIHGALANIGIVRSEGPALTGAVAAVLDGVRAGELVGEDEITTLFSARGPEVLAVAA
                     LADQLRRQVVGDDVTWVSNRNINYTNVCTFKCKFCGFSKGPLSLNLRGKPYLLTLEEI
                     AGRVSEAWDMGATEVCLQGGIHPDFDGEYYLDVTRAVKEAAPDIHVHGFTALEVTEGA
                     KRNGEPLADYLRRMMDAGLRTLPGTAAEILDDEIRKILCSDKIDTQEWLDAHRTAHQV
                     GLRSNVTIMFGSIESPRSWARHIVATRDLQAETGGFTEFVPLPFVHMASPIYLQKRSR
                     RGPTFRESILMHAVGRIAYHGSIDNIQASWVKLGLGGVRQLLAAGVNDLGGTLINENI
                     SRAAGAEHGQMLTEADFAALVEPMGRTLVQRTTLYGRPEPRDLPPQKARIPVAVTS"
     gene            3829..4956
                     /locus_tag="PKY13_10515"
     CDS             3829..4956
                     /locus_tag="PKY13_10515"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOT80412.1"
                     /translation="MTGEPHGARPTDNSPRSADPGAAPPPPVTAPQPSRGRSSRPRTG
                     NPAIDQAILDLIATTGVSDDVDVITDLVTTAVRFGLDGNDRGELKLISSALRDLRRAA
                     NQFRPYRSRRKCSIFGSARTPEGTAAYAAAKQMGPPLAANDWMVITGGGPGIMTAGVD
                     GAGRDNSFGVTIRLPFEPMDAGGIVPDERLVRFRHFFSRKLTFMRESSAYVVFPGGFG
                     TLDETFELLTLIQTGKEPPVPVVLFEPEGDTYWKLWKEFVNDELLVAGLVSPVDLDLV
                     RITSSVTEAMEYIDGFYRVFHSMRYVDGRLVLRLESDVSDATLQRLNAEFSDVLVEGE
                     IERTTALPPEVEDDDEVWRPRLLLAFDNWHFGRLHQLVRAL"
     gene            complement(5089..5304)
                     /locus_tag="PKY13_10520"
     CDS             complement(5089..5304)
                     /locus_tag="PKY13_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80413.1"
                     /translation="MSDGAFQEIRDRLEVIAEELADLSMERLRRSIDVGGDQLPIDEK
                     RLSRARRAVLKAADILSEPDRRAGFDD"
     gene            5330..6100
                     /locus_tag="PKY13_10525"
     CDS             5330..6100
                     /locus_tag="PKY13_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80414.1"
                     /translation="MAASPTAPVTHEWVGFEEPNGATWLFDATFLLSSWTCIFGNGCQ
                     GVLTADASDLAQGCCSYGAHFADKADRKTVTDAAKHLTPATWQFAAETKAAGGPITRN
                     DDGDWVTRQIDDACCFLNRPEFEAGPGCALHSVAIAHGERPMDWKPDVCWQLPLRLLS
                     ETDQNGHVTNTLREWKRRDWGEGGEDFHWWCTDAPDAFVGHRAVYEEMRDEIVELVGQ
                     EAYDWFVAYAESLPPTTIRPHPAAQPLPDPVRRGRRKS"
     gene            6133..>6951
                     /locus_tag="PKY13_10530"
     CDS             6133..>6951
                     /locus_tag="PKY13_10530"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT80415.1"
                     /translation="MAAFTSSELRNAVLDDGLLLPEFVCDQMVAAIDSGKHVILTGPP
                     GTGKTTLAYITAELGRRAMMCTGYLPTTATSEWTTYETIGGMQPTLEGLIYRPGMFVD
                     AIESGRWLVIDEMNRSNFDRAFGQLFTVLSGSAVVLPFKRAGQSRRISIVPFGEEAPA
                     GTDPIRVPASWRIIATMNAFDKNLLYDMSYALMRRFAFIEVGTPTDATFETLLDGPGD
                     IVKKLLPLRRFVDLGPAVFIDASRYAERRLRDDISRSRVLFEAFYAYFLPQLDGI"
BASE COUNT         1106 a         2556 c         2262 g         1027 t
ORIGIN      
        1 tcgccgctgt tgccgatggc gatcaggacg ttgcggacga agcggtcgcg gccggtgcgc
       61 ttcacgggcg tgccgcggaa gcgggcgcgg aaggccgcgt cgtcgagcgc caggagatcg
      121 gcgagcggcg ggttgtcgag ctcggcgcgg acgtcctcgc cgccggagat gcgccactcg
      181 acccacgcga agagggacag cgggaggatc atcacgaggc cgcggaaccg ttcgaagagt
      241 cggacgagca atggcgcgaa cagggtcaac acgaggaaag ccgacatgaa ccacaggtgc
      301 ccggtgtggc ggcgcatgaa gagggagccc tcgggcacca cgaagcccgt cgccccgagc
      361 agcaggtcgt cgtggtggac cgtccagtag tcgctcgtgt ggccggcgtc cgcgacgaca
      421 gcgacgagca ccatcaccgc gtagtaggcc cagtacggca ccagcagacg gcggaacgtc
      481 gagcgccagt aggtgagcgc ggaccgacgg gctccgtcga cggtgccgag ggtcttgcgc
      541 accagcgcac ccgagacgaa gaacaccatc ggcatgcccg gcaggacgta ggtgggggcc
      601 ggccagaacc agaaaccgga tcgaccgatc aggtggatac cgatcacgcg ggtgatcgac
      661 aggctccgca gcaggtcgag cagaccgtcg cgagggcggc cggccggcga ggcgggcgac
      721 gatcggcgcg acggtgaggt ctgcgtcatc agggttctcc gggaggggcg ggcagcaatg
      781 acacggcccg atgtcacccg gagccgaggg ggagcctatt gcgtcgatcc cccacaccgc
      841 ccatctgtgg gctccgtgcg cccgagtcgg ttcgcgatgt gaactcacgt cggttcgttt
      901 tccgaactaa ggtcccccgg catgtcccag gtcaccgaat ccccgccgat tcccgacgcg
      961 cccgagcccg acgccggcgc ggccccggcg gccgaactcg actgctcgat cgcccgagca
     1021 ctcggtgtca tcggcgaccg ctggaccatc ctgatcatgc gggacgcgtt ccggggtatc
     1081 cgccggttcg acgacttccg gcgcgatctc gacatcgccc ggccggtcct cgccgaccgc
     1141 ctgaagaagc tggtcgatgc cgggatcatg gtgcgacgcc gctaccagac cagccccgag
     1201 cgcttcgagt accgcctcac gcccatgggc atggagctgt caccagcact cgtcgccctc
     1261 atgcgctggg gcgaccgctg gctcaacgac ggccgctctc ccaccgtgct cgttcacgaa
     1321 ccctgcggat ccgagctcga gcagggcttc tggtgccgca cctgccaaca gaccttcaca
     1381 cccggcgaca tcgccagccg caacccccac tccgccaccc cccactcgtc gggagccaca
     1441 catgtccacc actgaccgct cgctcaccga cgtgccgttg gccgaactcc tcgccgaggc
     1501 cgctgcccgc cgcgacgcgc accacggcaa ccgggtgacc tactcgccca aggtgttcat
     1561 cccgctcacg atgttgtgcc aggacaagtg cgggtactgc acgttcgccc agccgccggc
     1621 acgcctcgat gcgccgtacc tcacgcccga gcaggtgctc gccatcgctg cggccggcgc
     1681 cgaggtcgga tgccacgagg cgctgttcac cctcggtgag cgcccggagc tccggtatcc
     1741 cgtcgccgcc gagtggctcg ccgagcacgg atacgcgtcg accgtcgact acctcgcggc
     1801 gatgtgcgcg ctggtccgcg acgagaccgg gctgctcccc cacgccaacg ccggcgcgct
     1861 cacccgcgac gagctggcgc agcttcgcga ggtgtcggcc agccagggca tgatgctgga
     1921 atcgctgaac ccgaacctcg acgcccatcg tgggtcgccc gacaagacgc ccgaacgtcg
     1981 gttggcgacg ctcgaatggg cgggcgagct ccagatcccg ttcacgacgg ggatcctcgt
     2041 cggcatcggc gagtcccgct ccgaccgggt cgacgcgctg gaggccatcg ccgagtcgca
     2101 tcgccgccac ggccacgtgc aggaggtcat cgtccagaac ttcctcccca agcccggcac
     2161 ctcgatgcac gcagcacgcc cgtgcccgac cgacgactac ctcgacgcca tcgccctggc
     2221 ccggctcatc ctccccgccg acgtccacct gcaggtccca ccgaacctca ccgacgactt
     2281 cggttcgctc ctcggcgccg gcatcgacga ctggggtggc gtgtcgccgg tgaccgccga
     2341 ccacgtgaac cccgaacggc cctggccggc gatcgaccgg ctccgcgccg tgaccgaagc
     2401 ggccggccac accctggcgc cgcgcctcac gatctacccc gagttcgccg ccgccccgga
     2461 gcgttggctc gccgggccca tgcggttccc ggtgctcgac cgctccgacg ccgagtcact
     2521 ggcccgcgat cgcgacaccc tcgacgagca ccccggcgcc accgtctggt actcgggcgc
     2581 cgacaccgat ccgcccgtcg tcatcgagac gtcctcggtc accatccacg gcgcgctcgc
     2641 caacatcggc atcgtccggt ccgagggtcc ggcactcacc ggcgctgtcg ccgcggtgct
     2701 cgacggcgtc cgtgccggcg agctggtcgg cgaggacgag atcaccaccc tgttctcggc
     2761 ccgcggcccc gaggtgctcg ccgtggccgc gttggccgac cagctgcgcc ggcaggtggt
     2821 cggcgacgac gtcacctggg tgtcgaaccg caacatcaac tacacgaacg tgtgcacgtt
     2881 caagtgcaag ttctgcggat tctcgaaggg tccgctctcc ctcaacctcc gcggcaagcc
     2941 gtacctcctc accctcgagg agatcgccgg tcgcgtcagc gaggcgtggg acatgggcgc
     3001 gaccgaagtg tgcctgcagg gaggcatcca ccccgacttc gacggcgagt actacctcga
     3061 cgtcacccgc gccgtgaagg aggcggcgcc cgacatccac gtgcacggat tcaccgcgct
     3121 cgaggtcacc gagggcgcga agcgcaacgg cgagccgctc gccgactacc tgcgccgcat
     3181 gatggacgcc ggtctccgca cgctccccgg taccgctgcg gagatcctcg acgacgagat
     3241 ccgcaagatc ctgtgctccg acaagatcga cacccaagag tggctcgacg cccatcgcac
     3301 cgcccaccag gtcggcctcc gctccaacgt gacgatcatg ttcgggtcga tcgagtcgcc
     3361 gcggagctgg gcgcgccaca tcgtcgccac ccgtgacctc caggccgaga ccggcgggtt
     3421 caccgagttc gtgccgttgc cgttcgtgca catggcgtcg ccgatctacc tccagaagcg
     3481 gtcgcggcga gggccgacgt tccgcgagtc gatcctgatg cacgccgtcg ggcgcatcgc
     3541 ctaccacggc tccatcgaca acatccaggc gtcgtgggtg aagctcggac tcggcggggt
     3601 gcggcagctg ctggccgccg gcgtcaacga tctgggcggc accctcatca atgagaacat
     3661 cagccgtgcc gccggagcgg agcacggcca gatgttgacc gaggccgact tcgcggcgct
     3721 cgtcgagccg atgggtcgga cgctggtcca gcgcacgacg ctgtacggcc ggcccgagcc
     3781 tcgcgacctg ccgccgcaga aggctcgcat ccccgtggcg gtcacctcgt gaccggcgag
     3841 cctcacgggg cccggccgac cgacaacagt ccccgctcgg ccgatccggg cgctgctccg
     3901 ccccctcccg tcacggcccc ccagccgtcc cggggtcgga gcagccgccc acgcaccggc
     3961 aacccagcga tcgatcaggc catcctcgac ctcattgcga ccaccggagt gtccgacgac
     4021 gtcgacgtga tcaccgacct ggtcaccact gcggtccggt tcggcctcga cggcaacgac
     4081 cgtggcgagc tcaagctcat ctcgagcgcc ctccgggacc tccgccgcgc cgccaaccag
     4141 ttccggccgt accggtcccg tcggaagtgc tcgatcttcg gctccgcccg caccccggag
     4201 ggcacggccg cctacgccgc cgccaagcag atgggcccgc cgctcgccgc caatgactgg
     4261 atggtcatca ccgggggtgg ccccgggatc atgaccgccg gcgtggacgg cgctgggcgc
     4321 gacaactcgt tcggtgtgac cattcgcctg ccgttcgaac cgatggacgc cggaggcatc
     4381 gtgcccgacg agcgcctggt ccggtttcgt cacttcttca gccggaagct caccttcatg
     4441 cgggagtcct cggcgtacgt ggtgttcccg ggcggcttcg gcaccctcga cgagacgttc
     4501 gagctgctga cgctcatcca gaccggcaag gaaccgccgg tccccgtggt gctgttcgag
     4561 cccgagggcg acacgtactg gaagctgtgg aaggagttcg tcaacgacga actcctcgtc
     4621 gccggcctcg tctcccccgt cgacttggat ctggtgcgca tcacgtcgtc ggtcaccgaa
     4681 gccatggagt acatcgacgg cttctaccgg gtcttccatt ccatgcgcta cgtggacggt
     4741 cggctcgtgc tgcgcctcga gagcgacgtg agcgacgcga cgctgcagcg gctgaacgcc
     4801 gagttctccg acgtgctcgt ggaaggcgag atcgagcgca cgacggcgtt gccgcccgag
     4861 gtcgaggacg acgacgaggt ctggcgcccg cgcctcctgc tcgcgttcga caactggcac
     4921 ttcggccgcc tccaccagct cgtccgcgcc ctctgacccc cacccgtccg gagcgctgac
     4981 gtgctctgga gcgcccaggt gacagaaact gtcgcaccag cgctccagag caactgggcg
     5041 ctccagccgg gttggtgcgg ggtgcggggg taagggcgga ggaatcggtc agtcgtcgaa
     5101 gccggcgcgg cggtcgggtt cggacaggat gtccgccgct ttgagcacgg cgcgacgggc
     5161 acggctcaac cgcttctcgt cgatcggaag ttgatcgcct cccacgtcga tcgagcggcg
     5221 caaccgctcc atcgacaggt cggccaactc ctcggcgatg acctcgaggc ggtcacgaat
     5281 ctcctggaag gcaccgtcgc tcacgggggc agtctagatt ctgcctccca tggcagcttc
     5341 cccgaccgcc cccgtcaccc acgagtgggt cggcttcgag gagcccaacg gcgccacgtg
     5401 gttgttcgac gcgaccttcc tcctcagcag ctggacgtgc atcttcggca acggttgcca
     5461 gggtgtgctc accgctgacg cgtccgacct ggcccagggc tgctgcagct acggcgccca
     5521 tttcgccgac aaggccgacc gcaagaccgt gaccgacgcc gccaagcacc tcaccccggc
     5581 gacctggcag ttcgcagcgg agacgaaggc ggccggcggc ccgatcaccc gcaacgacga
     5641 cggcgactgg gtgacccgcc agatcgacga cgcgtgctgc ttcctcaacc gtcccgagtt
     5701 cgaggccggc cccgggtgcg ccctgcactc ggtggcgatc gcccatggag agcggccgat
     5761 ggactggaag cccgacgtgt gctggcagct ccccctccgc ctcctcagcg aaaccgacca
     5821 gaacggtcac gtgaccaaca cgctccgcga gtggaaacgc cgcgattggg gcgagggcgg
     5881 cgaggacttc cactggtggt gcaccgatgc gccggacgcg ttcgtcggcc atcgggcggt
     5941 ctacgaggag atgcgcgacg agatcgtcga gctcgtcggc caggaggcgt acgactggtt
     6001 cgtggcctac gccgagtcgc tcccgccgac cacgattcgg ccgcatccgg cggcgcagcc
     6061 gctcccggac ccggttcggc gaggtcgccg caagtcttaa gcacgccgcg gttcgtgccg
     6121 acagtggagg ggatggctgc cttcacgagt tcggaactcc gcaacgcggt gctcgacgac
     6181 gggctgctcc tgcctgaatt cgtctgcgac cagatggtcg ctgccatcga cagcggcaag
     6241 cacgtgatcc tgaccgggcc ccccgggacc gggaagacga ccctcgcgta catcaccgcc
     6301 gagctcggtc gacgggcgat gatgtgcacg ggctacctgc ccaccacggc cacgtcggaa
     6361 tggacgacct acgagaccat cggcggcatg cagccgacgc tcgaaggcct gatctaccgc
     6421 cccggcatgt tcgtggatgc catcgagtcg ggccgttggc tcgtcatcga cgagatgaac
     6481 cgctcgaact tcgaccgcgc cttcggccag ctcttcaccg tgctctcggg atccgcggtc
     6541 gtgctgccgt tcaagcgcgc cggccagagc cgacgcatct cgatcgtccc gttcggcgag
     6601 gaggcccctg cggggaccga tccgatccgg gtgccggcgt cgtggcggat catcgccacc
     6661 atgaacgctt tcgacaagaa cctgctctac gacatgtcgt acgccctcat gcgacgcttc
     6721 gcgttcatcg aggtcggtac gccgacggac gcgacgttcg agacgctgct cgacggaccc
     6781 ggcgacatcg tgaagaagct gctcccactc cgtcggttcg tcgacctcgg gcctgccgtg
     6841 ttcatcgacg cctcgcggta cgccgagcgg cggctgcgtg acgacatcag ccgctcccgc
     6901 gtgctgttcg aggccttcta cgcctatttc cttccccagc tcgacggcat c
//
LOCUS       DAOSOA010000161         6947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10947_length_6947_cov_4.376524, whole genome shotgun sequence.
ACCESSION   DAOSOA010000161 DAOSOA010000000
VERSION     DAOSOA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10947_length_6947_cov_4.376524"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PKY13_10535"
     CDS             complement(<1..1143)
                     /locus_tag="PKY13_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOT80416.1"
                     /translation="MDLISRRTVLSAVAWLPLGWGLAGCAGDDRVDATLPSGRQLVMP
                     KELRSVDGVLDIDLRVARADLAAGSGRRFAYTYDGTSPGPTLRVRPGDTVTVRLRNDL
                     DESTNLHTHGLHVDPSGSSDNVFVEVQPRSTHTYTYRIPDDHRSGTFWYHPHHHGMVA
                     AQVSAGLFGVIIVEDDLDTLPDLGDAAERLLVLNDPRVGSDASVLDVSAMEVMQGREG
                     DATLVNGQDEPVIPAETGRLEHWRIVNASPSRYYRMALEGHALHLIGTDGGRLSEPTT
                     VDEVLIAPGERVEVMVALTEGRHELRSLGYDRGSAGMGGGMMGGRSGAGSGPQVLAVV
                     DTTVTSVVPPVPGSLADPATTRLPGPTASRRLELAMGMGGGMGGGMM"
     gene            complement(1180..2094)
                     /locus_tag="PKY13_10540"
     CDS             complement(1180..2094)
                     /locus_tag="PKY13_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOT80417.1"
                     /translation="MTLLGLLLPALASVAFARSADRIVGVDPRRSVWRLAVGGSILAV
                     AAVASAAIAAWVLVARIPLIASIGGWDVRRVQGSVDVPVFVSIVGLVVLVVAGARLFA
                     ASSRAMCGWRAVLGLHRSLRPGPRIIDASVVAGAAVAVPALPGHPGRIVLDSALLDAL
                     EPAERTAVIAHETEHLDARHAWASTVVALAVAANPLLRSLGPVHALAVERAADEQAAA
                     RTDRRTTARAVAMAALHASTTQRLILGIGSSVTAIRVEALLAEPTGGRGRPALFFALP
                     FLAALGALAAGVRMETVFEALMRVTGSA"
     gene            complement(2091..2480)
                     /locus_tag="PKY13_10545"
     CDS             complement(2091..2480)
                     /locus_tag="PKY13_10545"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOT80418.1"
                     /translation="MPGRPPIERRSRRRSGQLENEVLAALWSADGALTAGEVQEQVPG
                     DLAYTTVMTILARLHEKGVIERAKVGRAFAYRPVVAESDAVAQQVREMLDHSGDRRAA
                     LLGLVAGLEPGEEAVLRELLSRAEGQP"
     gene            2666..3592
                     /locus_tag="PKY13_10550"
     CDS             2666..3592
                     /locus_tag="PKY13_10550"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT80419.1"
                     /translation="MLLTGATRAAAEPSGNSVAAEHRDHDTATGPSTLTTGKAVVLGL
                     VEGATEYLPVSSTGHLLISERILDVGQSPAEKSVTDTYTVVIQIGAIVAVLGIFRKRF
                     KLMLDGVLHADPGGRSLLRSLLVAFIPAAIIGKLFGDPIKEHLLRPWPVVGAWVVGGI
                     VILAFVANRSRFRVRIDSLQAIPPTTALAIGAAQTVALWPGTSRSFVTILAALALGCS
                     MTVAVEFSFLLGFVTLSAATALELAQNGSEMMDHFGILNPIIGIVVAGVAAFASVRWM
                     ITYLERHPLTIFGWYRIALGLGVGVLLVTQTI"
     gene            3788..4468
                     /locus_tag="PKY13_10555"
     CDS             3788..4468
                     /locus_tag="PKY13_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT80420.1"
                     /translation="MNARLLIVEDDAGIRSGLVRALTNAGYDCAASASAEAARAADRP
                     DLVLLDLGLPDGDGMDLALEFQARWPTVPIIMVTARAEEMDVVAGLDTGAVDYITKPF
                     RLAELLARVRAQLRKPASPPEQPVLRSAGVEVDRQARTVTVSGEPVMLRAKEFDVLVT
                     LMTNEGKVVTREDLMSQVWDEHWFGSTKTIDVHISSLRRRLGEAVGEASRITALRGVG
                     YRWERDDA"
     gene            4502..5797
                     /locus_tag="PKY13_10560"
     CDS             4502..5797
                     /locus_tag="PKY13_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT80421.1"
                     /translation="MRRRVLTAIVAVTLLATLVLTVPLTLLIAAREHEDADQELALAA
                     ERGANELTFNDLNSGGLRLPTFERALSVAVYAPDGRRLAGNGPRTADRFTQATKLLTR
                     TGTTSTTRILVRPVVDNEVKVAMIRVAEPLAEAERRAWRDIAILLGIDVAAVAIAAAV
                     GAWVSTRLVRPLTAIRDDAIRLGDGDFSIEPAPSGIAELDDTAAALAETASRLNGILQ
                     RERAFTADASHQLRTPLTSLRLAIETELATPSTDPTPMLEEALGEIDRLEDTISTLLS
                     VARDQPISREPLDLADWLADVERRWSLVATERQRLLHCTCTGDAHPHVSRAVLDHVTD
                     VLVSNAIQHGQGTVSVTVESEPDGAALTVADVGRLHRNPGDLFTRRDPGASGHGVGLS
                     LARTLAEAEGGRLTIVSTSPTTFRLVLPGRGLTGDRTAP"
     gene            5928..6722
                     /locus_tag="PKY13_10565"
     CDS             5928..6722
                     /locus_tag="PKY13_10565"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80422.1"
                     /translation="MTRAVTPPASVGRTLLNKVPEVTLLFWVIKIMATTVGETAADYL
                     AGNLGFGLTATTLIMTVLLGGALVLQFRRPRYVPWVYWLAIVLISVVGTLFSDNLVDN
                     FGVSLWTTTALFGVAMAIAFGAWYRTEGTLSIHHVDTPKREAFYWIAILFAFALGTSA
                     GDLISEKLDLGYWKSIVLFGALIALVAAGRFVAKLSATLTFWVAYVLTRPLGASIGDY
                     LSQKRRDGGLGLGTTGTSIIFLVIIVGLVGWFTIEQRSDTKELTPA"
     gene            6719..>6947
                     /locus_tag="PKY13_10570"
     CDS             6719..>6947
                     /locus_tag="PKY13_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HOT80423.1"
                     /translation="MTDSTNESEPTQRPAARRRVALFVGALLLVAVGIQFIPYGVDNP
                     SARDEPKWDSARTRQLFMTACADCHSNQTKIL"
BASE COUNT         1161 a         2600 c         2182 g         1004 t
ORIGIN      
        1 catcatgccg ccacccatcc cgccacccat gcccatagcc agctcgagac gacggctggc
       61 ggtcgggccg ggcaggcgtg tcgtagcggg atcggcgagc gacccaggca cgggcgggac
      121 caccgacgtc acggtggtgt ccacgaccgc gagcacctgc gggcccgacc ccgcaccgga
      181 gcggccgccc atcatccctc cacccatgcc ggccgagccc cggtcgtagc ccagcgaccg
      241 caactcgtgt cggccctcgg tcagcgccac catgacctcc acccgctccc caggagcgat
      301 cagcacctcg tcgaccgtcg tcggttccga cagacggccg ccgtcggtgc cgatcaggtg
      361 gagcgcgtga ccctccagcg ccatccggta gtagcgcgac ggcgaggcgt tgacgatgcg
      421 ccagtgctcg agccggccgg tctccgctgg gatgacgggt tcgtcctgcc cgttgaccaa
      481 ggtggcgtcg ccctcccggc cctgcatcac ctccatcgcc gacacgtcga gaacggaggc
      541 gtcggatccg acccggggat cgttcagcac cagcagccgt tcggcggcgt cgccgagatc
      601 cggaagggtg tcgaggtcgt cctcgacgat gatcacgccg aacaacccgg cggacacctg
      661 cgccgcgacc atcccgtggt gatgggggtg gtaccagaac gtcccggaac gatggtcgtc
      721 ggggatccgg taggtgtagg tgtgggtgga gcgcggttgg acctccacga acacgttgtc
      781 gctcgacccg ctcgggtcga cgtggaggcc atgggtgtgc aggttcgtcg actcgtccag
      841 gtcgttgcgc agacggaccg tcaccgtgtc gcccgggcgg acccgcagcg tcggacccgg
      901 cgaggttccg tcgtaggtgt acgcgaaccg ccgtccggac cctgcggcga ggtcggccct
      961 agcgacccgc aggtcgatgt cgagcacgcc gtcgacgctt cgcaactcct tcggcatgac
     1021 gagctgacgg cccgagggaa gcgtggcgtc gacccgatcg tcgccagcgc atccggccag
     1081 accccagccc aacggcaacc aggcgacagc gctcagtacg gtacgccgag agataagatc
     1141 cataccccca agggtatctg ccccacaacc cggacgcagt caggcagacc ccgtgacgcg
     1201 catgagcgct tcgaacacgg tctccatgcg aactcctgcc gccagcgcac cgagggcggc
     1261 caggaacgga agggcgaaga acagcgctgg ccgaccgcgg ccgccggtgg gctcggcgag
     1321 caacgcctcg acacggatcg ccgtcaccga cgagccgatg ccgaggatca gccgctgcgt
     1381 cgtcgaggcg tgcagcgctg ccatcgccac cgcccgagcg gtcgtccgcc gatcggttcg
     1441 tgctgcggcc tgctcgtccg cagctcgttc gactgccaag gcgtggaccg ggcccagcga
     1501 gcgaagcagc gggttcgccg caaccgcaag agcgacaacg gtcgacgccc acgcgtgccg
     1561 ggcgtcgagg tgctcggtct cgtgggcgat caccgccgtg cgctcagccg gttcgagcgc
     1621 atccaggagc gcactgtcga gaacgattcg acccggatga ccggggagag ccggcaccgc
     1681 gactgcagcg cctgcgacga ctgaagcgtc gatgatcctc gggccgggcc gcagggaacg
     1741 atgcagcccg aggacggcgc gccacccaca catcgcacga gacgacgcgg cgaacagtcg
     1801 agctccagcg acaacgagca cgacgagacc gacgatcgag acgaacacgg gaacgtcgac
     1861 cgagccctgc acacgccgga cgtcccaccc accgatcgac gcgatcagcg ggatccgagc
     1921 aaccagcacc cacgcagcga tcgcggccga agccacggcg gcgaccgcca ggatcgaccc
     1981 gccgaccgcg agccgccaaa cactgcgtcg tgggtcgact ccgacgattc gatcggcgga
     2041 ccgagcgaac gcgacgctcg ccagcgccgg gagcagcagc ccgaggaggg tcacggctga
     2101 ccctcggcac gcgagagcag ttcgcggagc acggcttcct ctcccggctc gagcccggcc
     2161 accaacccga gcagcgcggc ccgccgatca ccactgtggt cgagcatctc gcgaacctgt
     2221 tgcgccacgg cgtccgactc ggccacgacc ggacgatagg cgaacgcccg accgaccttg
     2281 gcccgctcga tcacgccctt ctcgtgcagc cgggcgagga tcgtcatcac cgtcgtgtag
     2341 gccagatcgc cgggcacctg ctcctgcacc tcgccagcgg tgagcgcgcc gtcggctgac
     2401 cacaacgccg cgagcacctc gttctccaac tgccccgagc ggcgtcggga gcgtcgttcg
     2461 atcggaggac ggcccggcat cgacccagac tacgacacgt gtcgtagttc gcgctaccgt
     2521 acgacagctg tagtagttga gtgaggagac gacgatgcgg acaacgacat cgagaacacg
     2581 aacgcgaacg ccgagaacgc gaacaacgag aacgggaacg gcggggcaac gtcgtcggat
     2641 cctcctgctg atcgtcggcg cggcggtgct cctcaccggc gcgacccggg ccgcggccga
     2701 accctcgggc aacagcgtgg ccgccgagca ccgcgaccac gacaccgcga ccgggccatc
     2761 gaccctgacc acgggcaagg ccgtggtcct cggtctcgtc gaaggcgcga ccgagtacct
     2821 gcccgtgagc tccacaggcc acctcctcat cagcgagcgc atcctcgacg tcggccagtc
     2881 ccccgcggag aagtcggtca ccgacaccta caccgtcgtc atccagatcg gtgccatcgt
     2941 cgccgtgctc ggcatcttcc ggaagcggtt caagctcatg ctcgacggcg tcctccacgc
     3001 cgaccccggc ggccgttcgc tcctgcggtc actcctcgtg gccttcatcc ccgccgccat
     3061 catcggcaag ctcttcggcg acccgatcaa ggaacacctg ctccgaccat ggcccgtcgt
     3121 cggagcatgg gtcgtcggcg gcatcgtcat cctcgccttc gtggccaacc ggagccggtt
     3181 ccgcgtccgc atcgactcgc tgcaggcaat ccccccgacg accgctctgg ccatcggcgc
     3241 agcgcagacc gtggcactgt ggcccggcac gagccgcagc ttcgtcacca tcctcgccgc
     3301 cctcgccctg ggttgctcca tgaccgtggc cgtcgagttc tcgttcctcc tcgggttcgt
     3361 caccctcagc gcagcgaccg ccctcgaact ggcgcagaac gggtccgaga tgatggacca
     3421 cttcggcatc ctcaacccca tcatcggcat cgtcgtcgcc ggcgtggccg cgttcgcctc
     3481 cgtccgctgg atgatcacct acctcgaacg ccacccgctg acgatcttcg ggtggtaccg
     3541 gatcgccctc ggcctcgggg tcggcgttct cctcgtgacc cagacgatct gactcgacgc
     3601 ccttgtgttc tcccggtctc gtcggtggat cgctccggaa cggccaccca aatagccggg
     3661 ctcgggaacc ggcacagacc cgcctgttgg gcatggcgca accggcgcgc gagcggggtg
     3721 tctaactgtc ctctgaccat cggtccgcca gacttgccaa catgttggac gtcgggcagc
     3781 gacccggatg aacgctcgct tgctgatcgt cgaggacgac gcgggtatcc ggtcggggct
     3841 ggtgcgagcc ctgaccaacg ccggctacga ctgcgccgcg agcgcatcgg ccgaggcagc
     3901 gcgcgccgcc gaccggcccg acctggtgct gttggacctg ggcctccccg acggcgacgg
     3961 catggatctg gctctggagt tccaggcccg ttggccgacg gtcccgatca tcatggtgac
     4021 cgcccgcgcc gaggagatgg acgtggtcgc cggcctcgac accggagcgg tcgactacat
     4081 caccaaaccg ttccgcctcg ctgaactgct ggcacgggta cgtgcccagc tccgcaagcc
     4141 ggcgtccccg cccgagcagc cggtgctccg gagcgcaggg gtcgaggtcg atcggcaagc
     4201 ccgcacggtg acggtcagcg gcgagccggt gatgctgcgg gccaaggagt tcgatgtcct
     4261 ggtgaccttg atgaccaacg agggcaaggt cgtcacccgg gaggacctga tgtctcaggt
     4321 gtgggacgag cactggttcg gttccaccaa gaccatcgac gtccacatca gctcgctccg
     4381 ccgtcgcctc ggagaggccg tcggggaggc gtcgcggatc actgcacttc gcggtgtcgg
     4441 gtaccgctgg gaacgcgatg acgcgtgacc ggcgagccgt cccgacgtcg aggtcagccc
     4501 gatgagacgc cgggtactga cggcgatcgt tgcggtcacg ctgctcgcca ccttggtctt
     4561 gacggttccg ctgaccctgt tgatcgctgc ccgtgagcac gaggacgccg accaggagct
     4621 ggccctcgcc gccgaacgag gcgccaatga gctgaccttc aacgacctga acagcggagg
     4681 cctccgtctc ccgacgttcg aacgggccct gagcgtcgct gtctacgccc ccgacggacg
     4741 gcgcctcgcc ggaaacggac cccgcaccgc cgaccggttc acgcaggcga ccaagctgct
     4801 tacgagaacc gggaccacgt ccacgactcg catcctggtc cggcccgtcg tcgacaacga
     4861 ggtgaaggtc gcgatgatcc gagtggccga gccgctcgcc gaagccgaac gacgcgcgtg
     4921 gcgagacatc gccatcctcc tcggcatcga cgtcgcggcg gtcgccatcg ccgcagccgt
     4981 cggtgcctgg gtgtctactc gtctcgttcg accgctgacc gccatccggg atgacgccat
     5041 ccgcctcggc gacggcgact tctcgatcga accagcaccc agcggcatcg ccgagctcga
     5101 cgacaccgcc gcagccctcg ccgagaccgc ctcacgactc aacgggatcc tgcaacgcga
     5161 gcgggccttc accgcagacg catcacacca actccgaaca cctttgacgt cacttcgcct
     5221 cgccatcgag accgagctgg cgacccccag caccgacccc accccgatgc tcgaggaggc
     5281 gttgggagag atcgaccggc tcgaggacac catctccacg ctcctcagcg tcgcccgcga
     5341 ccagcccatc agccgcgaac cgctcgacct cgcggactgg ctcgcggacg tcgaacgcag
     5401 atggtcgctc gtcgccaccg aacggcaacg gctcctgcac tgcacctgca ccggtgatgc
     5461 ccatcctcac gtgagtcgcg ccgtgctcga ccacgtcacc gacgtcttgg tgtccaacgc
     5521 catccaacac ggccagggca cggtatcggt caccgtcgaa agcgagcccg acggggccgc
     5581 tctcaccgtc gccgacgtcg ggcgcctcca ccggaacccc ggagacctgt tcacgcgacg
     5641 tgaccccggt gccagcggcc acggcgtcgg gctgtcactg gcacgaacac tcgctgaggc
     5701 cgagggcgga cgcctcacga tcgtcagcac ctcaccgacg acgttccgac tcgtgctgcc
     5761 cgggcgtggc ctgaccggag accgcaccgc gccgtgacgg ccagccgacg gcaggagatt
     5821 tgtccgtcgt cgaaccgagc cttgaccgac cccgcaccgg tccctccgta cggtccccgg
     5881 tgatcccaga catcggctgg gaccacctgc gccggaagga tccacgaatg acccgtgccg
     5941 tcaccccgcc cgcctcggtc gggcgaaccc tgctcaacaa ggtccccgag gtcacgctcc
     6001 tgttctgggt catcaagatc atggccacca ccgtgggcga gacggccgcc gactacctgg
     6061 ccggcaacct cggcttcggg ctcaccgcca cgaccctgat catgacggtg ctgctcggcg
     6121 gtgcccttgt gctccagttc cgacggcccc gctacgtgcc gtgggtctac tggctcgcca
     6181 tcgtgctcat cagcgtcgtc ggcaccctct tcagcgacaa cctggtcgac aacttcggcg
     6241 tcagcctctg gaccaccacc gccctcttcg gcgtggccat ggccatcgcc ttcggcgcct
     6301 ggtaccgcac cgagggaaca ctgtcgatcc accacgtcga cacccccaag cgcgaagcct
     6361 tctactggat cgccatcctc ttcgccttcg ccctcggcac ctccgccggc gacctcatct
     6421 ccgagaagct cgacctcggc tactggaagt ccatcgtcct cttcggcgcc ctcatcgcac
     6481 tcgtcgctgc gggacgcttc gtggccaagc tcagcgccac gctcacgttc tgggtcgcct
     6541 acgtcctgac ccggcccctc ggcgcgtcca tcggcgacta cctctcacag aagcgccgcg
     6601 atggcggcct cggactcggc accaccggga ccagcatcat cttcctcgtg atcatcgtcg
     6661 gcctggtggg ctggttcacg atcgagcagc gcagcgacac caaggaactg acaccggcat
     6721 gaccgactcg acgaacgaat ccgagcccac gcagcggccc gcggcgcggc gccgcgtcgc
     6781 cctgttcgtg ggagcgctcc tgctggtcgc ggttggcatc cagttcatcc cctacggcgt
     6841 cgacaacccc tcggcccggg acgaaccgaa gtgggacagt gcccgcactc gccagctctt
     6901 catgacggcc tgcgccgact gccacagcaa ccagaccaag atcctgt
//
LOCUS       DAOSOA010000162         6946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_10951_length_6946_cov_4.992309, whole genome shotgun sequence.
ACCESSION   DAOSOA010000162 DAOSOA010000000
VERSION     DAOSOA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10951_length_6946_cov_4.992309"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PKY13_10575"
     CDS             complement(<1..701)
                     /locus_tag="PKY13_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80424.1"
                     /translation="MGAPKIAERLFRGYGPLIGFAALFTAMAVFVPSVGQEIREVTTS
                     ASAANSTADSDAADTEALGETTSSVPGTETTVAATDTTAGGTDTSGGGTTGSTGGGTT
                     KPGATVPGGGATGGTNTGPQALSACGAHQVPSDPYSPPCIKFTGANGGATSKGVSADT
                     ITVAVRIEAFSSGLVDAISKAAGADLPSEDEQDIRRTLDGLVEFFNRTYQFYGRKLKL
                     EIYNGRGDVLKEVLGG"
     gene            complement(701..2233)
                     /locus_tag="PKY13_10580"
     CDS             complement(701..2233)
                     /locus_tag="PKY13_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80425.1"
                     /translation="MPGRRRIRRAAWSLAVGCSASLLGGALLPAAVDAQVEGEPAGFI
                     PGEATAQASTLTLGLSPGGGKPLEIVLGKSTARYQNRTATAEGTALSLGLLQIFFGPS
                     SACGDRPPIIPADQLPPLTTNDSRRNQPMATALEVRSPGYNGTPTTPGQLGGVIGTQI
                     ANASATPQASTAGTTTVSQDYGILAIDNGSTQVTTQLSGGVRTATAVMTASRIRILGD
                     MVVINSPRWEASTTSGGTETATGTFTFASATILGIDRTVANFPGDFNDFASGVASFLS
                     ALGVSLQYPQLVTEPGRVTMTPIVVGLNNPPIGLDFIQPLLKAIAPLREESNRKMISE
                     DCNNQAILQVADLALGILSGSGAVSLSAGGVTAFTAATEFPEPPALEIPDLDAPVIDV
                     LAPVVTAPPAVVNDLTLSRSTGSTSSSFASDDLGSSVVESAPPIDTGIVETAPVAAPV
                     AEAAGPGFELPSAELVSRRIEPGSKGGTAALVGAGGLVGLLLLAGADRFVMKRTKREM
                     PD"
     assembly_gap    2538..2637
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2638..4218)
                     /locus_tag="PKY13_10585"
     CDS             complement(<2638..4218)
                     /locus_tag="PKY13_10585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOT80426.1"
                     /translation="MSRDTALPPGARAQPASRADVAGGTTEFQPSTGHSPAEVVKLPV
                     KHADGPFGSVRPRRKGSLSLRRRLVAFDLGSVLVGWGLALTLPLLPEINTNDGRVVAL
                     TGLAVLIQLIAIAGQRLHQARVAARRAAELSGIARSALFAGLFLAAFADIYGVAFPIA
                     RIIAGGLLTVALLSTCRAIFSSWLRTQRALGKYARNLLLVGTNAEAVSLKQLLDTHPE
                     LGYHVAGITGDEEGYRNHAWGVPYLGESASTLEAVVDIDADGVLIAASAIDGTELNGL
                     SRELLRHEVHVHLSSGLQGIDHRRLRPLPLAHEPLFYLERSTLSSGQMRVKRALDIVL
                     SATTLLLTSPILIVAAIAIKINDGGPVLFTQRRVGRNCTPFTVYKLRTMVPDAEARLN
                     EVLETLGNGRDNVLFKLDNDPRRTKVGRFLEATSLDELPQLFNVLNGSMSIVGPRPAL
                     PEEVAKFDDELMDRFNVPPGVTGLWQVEARDNPSFSAYRRLDLFYVENWNLLLDLVLM
                     METCSSVAARVLRRGKKSR"
     assembly_gap    4509..4608
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4642..5487)
                     /locus_tag="PKY13_10590"
     CDS             complement(4642..5487)
                     /locus_tag="PKY13_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT80427.1"
                     /translation="MELADILTLSSHGPDTFVGSGPRYPWGGLYGGQIVAQALEAAAA
                     TVEDHLVVHSLRAYFIRRGDQDEPVRYEVDRIRNGRSFATRRVVARQAIGAILNLEAS
                     FQAKEESPDLDVVTMDRALPGPEHLESTSWSPVFERAFVPEDQLEPDSRLGAGRAAAW
                     LRTSKPIGDDALQNFAALAYLSDDLPTDAVVRAHPIGAEPEELRYRVLFTASLDHTIW
                     FHRPMRADEWHLHDFTCHSFVGGRGLAIGHVFDLAGRHVATVAQEVLLRDTRDRADRA
                     TTERS"
     gene            complement(5569..>6946)
                     /locus_tag="PKY13_10595"
     CDS             complement(5569..>6946)
                     /locus_tag="PKY13_10595"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT80428.1"
                     /translation="DLTILEKSDGVGGTWRANTYPGAACDVPSHLYSFSFAPKKDWTR
                     KFAQQPEILSYFEDVTDRYDLRHRIRFGTEATSARFDEDAGEWHVTVADGTSLTADVL
                     ISGLGQLNRPFTPEIEGLAEFGGDVFHSARWNHDTDLAGRRVGVIGNGASTVQFLPPT
                     AEVAGHLTLFQRSANWILPKPDREFSPLERRIFRNVPFAERLFRWNIYWRLEKNFFFM
                     RRGSRLGKLIERVATKELRKIVSDDLPAEALIPDYPPGCKRILISNDYYASLLRPNVE
                     VELSHIDHIEPGAVVTADGTRHELDVLIFGTGFRSTEFLAPMEVTGRHGADLNTAWAK
                     GAKAHLGVAVAGFPNFFMLYGPNTNLGHNSIIFMIEAQARYISRAVRELRDDHLAWLD
                     VRDDVMDLYNTRIQHNADGTVWVADCDSWYKNEHGVVTNNWPAFTVSYWKRMRDFRSH
                     EFVRRART"
BASE COUNT         1033 a         2146 c         2469 g         1098 t
ORIGIN      
        1 ccaccgagca cctccttgag cacgtcgccg cgaccgttgt agatctcgag cttcagcttg
       61 cgtccgtaga actggtaggt gcggttgaag aactcgacga gcccgtcgag ggtgcggcgg
      121 atgtcctgct cgtcctcgga cgggagatcg gcgccggcgg ccttggagat ggcgtcgacg
      181 aggcctgacg agaaggcctc gatccgaacc gcgaccgtga tcgtgtcggc gctcacgccc
      241 ttcgacgtgg caccgccgtt ggcgcccgtg aacttgatgc acggcggcga gtaggggtcg
      301 ctcggcacct gatgggcgcc gcacgccgac agggcctggg ggccggtgtt ggtgccgccg
      361 gtggcgccgc ctccggggac ggttgccccg ggcttggtgg ttcccccgcc ggtcgagccg
      421 gtggttccgc cgccggaggt gtccgtgccg cctgcggtgg tgtcggtcgc ggcgacggtg
      481 gtctcggtgc cgggcacgga gctggtcgtc tcaccgagcg cctcggtgtc ggcggcatcc
      541 gaatcggcgg tcgagttggc cgcgctggcc gacgtcgtga cttcgcggat ctcctgaccg
      601 accgacggca cgaacacggc catcgcggtg aacaacgcgg cgaacccgat caggggcccg
      661 tagccccgga acagccgctc ggcgatcttg ggtgctccca tcagtcgggc atctccctct
      721 tggttcgctt catgacgaat cggtcggcgc cggccaacaa cagcagtccg accaggccgc
      781 cggcaccgac gagcgccgcc gtaccaccct tgcttccggg ctcgatgcgc cgtgacacca
      841 gttctgccga aggcagttcg aaccccgggc cggcggcctc ggcgaccggt gccgccacgg
      901 gggcggtctc gacgatgccg gtgtcgatcg gtggcgccga ctcgacgacg ctgctcccga
      961 gatcgtcgga ggcgaacgaa ctcgaggtcg aacccgtcga tcgcgagagg gtcaggtcgt
     1021 tgacgacggc cggtggcgct gtcacgaccg gtgcgagaac gtcgatcacg ggggcgtcga
     1081 ggtcggggat ctcgagggcc ggtggctcgg ggaactcggt cgccgcggtg aaggcggtga
     1141 ccccgccggc gctgaggctg acggcgccgc tgccgctgag gatgccgagc gccaggtcgg
     1201 cgacctgcag gatcgcctgg ttgttgcagt cctcgctgat catcttgcgg ttgctctcct
     1261 cgcgcagcgg cgcgatcgcc ttgaggaggg gctggatgaa gtcgaggccg atgggcgggt
     1321 tgttgagccc gacgacgatc ggcgtcatgg tgacccggcc cggctcggtg acgagctggg
     1381 ggtactgcag cgacacaccc aaggcgctga ggaagctcgc gaccccgctg gcgaagtcgt
     1441 tgaagtcgcc ggggaagttc gccacggtgc ggtcgatccc gaggatcgtc gcactggcga
     1501 acgtgaacgt gccggttgcg gtctccgtgc cgccgctggt ggtcgacgct tcccatcggg
     1561 ggctgttgat gacgaccatg tcgccgagga tccggatgcg gctcgcggtc atcacggccg
     1621 tggcggtgcg gaccccgccc gagagctggg tggtgacctg ggtggagccg ttgtcgatgg
     1681 cgaggatgcc gtagtcctgg ctgacggtgg tggtgccggc cgtgctggcc tgcggggtgg
     1741 cggaggcgtt ggcgatctgg gtgccgatga caccgccgag ctgacccggg gtcgtcggtg
     1801 tgccgttgta gccgggcgat cggacctcga gcgccgtcgc catcggctgg ttgcggcgcg
     1861 agtcgttggt ggtcagcggc gggagctggt cggcggggat gatgggcggg cggtcgccgc
     1921 acgccgacga cgggccgaag aagatctgca ggagcccgag cgacaacgcc gtgccctcgg
     1981 ccgtcgcggt ccggttctgg tagcgcgctg tggacttgcc gagcacgatc tcgagcggct
     2041 tgccaccgcc ggggctgagg cccagcgtga gcgtgctggc ctgcgcggtc gcctcgcccg
     2101 ggatgaagcc cgccggctcc ccctcgacct gggcgtcgac cgcggccggc agcagcgccc
     2161 cgccgagcag cgaggccgag cagcccacag cgaggctcca ggcggcgcgc cggatccgtc
     2221 gtcggccggg caccggtgtg cgccgtcggc cagtcgttgc cccctccgtt cccacgtcga
     2281 caccccccgg tggtcttcgc tcggtcttat tggcactgcg tcaatagacg atcggcacag
     2341 tatccgacgc gcctgctcgg cacacgcgaa ggactccgta ccccgagtcc cccgacccga
     2401 aacgtcttat tgaccgtagc tcaagaagcg ggcgccgcaa agcaccacgg cgttcgggct
     2461 ggcgtgccgc gacgactcag ccgcgcgact tcttgccgcg acgcaggacc cgggcggcga
     2521 ccgacgagca cgtctccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     2641 cgacttcttg ccgcgacgca ggacccgggc ggcgaccgac gagcacgtct ccatcatcag
     2701 caccaggtcg agcagcaggt tccagttctc gacgtagaag aggtcgagac gccggtaggc
     2761 cgagaacgac gggttgtcgc gggcctcgac ctgccacagg ccggtgacgc cgggcggaac
     2821 gttgaaccgg tccatgagtt cgtcgtcgaa cttcgccacc tcttcgggca acgccggacg
     2881 gggcccgacg atgctcatcg aaccgttgag cacgttgaac agctgcggga gctcgtcgag
     2941 actcgtcgcc tcgaggaacc gccccacctt ggtgcgacgg ggatcgttgt cgagcttgaa
     3001 caggacgttg tcgcgaccgt tgccgagcgt ctcgagcact tcgttgagcc gtgcctcggc
     3061 gtcgggcacc atcgtgcgca gcttgtacac ggtgaacggg gtgcagttgc ggccgacgcg
     3121 acgctgggtg aacagcaccg ggccgccgtc gttgatcttg atggcgatcg cggcgacgat
     3181 caggatcggc gacgtcagga gcaacgtcgt ggccgagagc acgatgtcga gcgcacgctt
     3241 gacccgcatc tgacccgacg agagtgtgct gcgctcgagg tagaagaggg gttcgtgcgc
     3301 cagcggcagc gggcgcagac gacgatggtc gatgccctgg aggccgctcg acaggtgcac
     3361 gtgcacctcg tggcggagga gttcccggct caggccgttg agctcggtgc cgtcgatcgc
     3421 gctcgcagcg atgaggacgc cgtcggcgtc gatgtcgacg accgcttcga gcgtggatgc
     3481 cgactcgccg aggtagggaa cgccccacgc gtggttgcgg tagccctcct cgtcgccggt
     3541 gatgccggcc acgtggtagc cgagctcggg atgggtgtcg agcagctgct tgagcgacac
     3601 agcttcggcg ttggtgccga cgaggagcag gttgcgggcg tacttgccga gggctcgctg
     3661 ggtgcgcagc cacgagctga agatggcgcg gcacgtgctg agcaacgcca ccgtgaggag
     3721 gccgccggcg atgatgcggg cgatcgggaa cgcgacgccg tagatgtcgg cgaacgctgc
     3781 caggaacagg ccggcgaaca acgccgaccg ggcgatgccg gacagctcgg cggcgcggcg
     3841 ggctgcaacc cgtgcctggt ggaggcgttg accggcgatg gcgatgagct ggatgagcac
     3901 ggcgagcccg gtgagcgcaa cgacgcggcc gtcgttggtg ttgatctcgg gcaggagcgg
     3961 cagggtgagg gcgaggcccc acccgacgag caccgagccg aggtcgaacg ccacgaggcg
     4021 tcgacgcagg gacagggagc ccttgcggcg gggccggacg ctgccgaacg gtccgtcagc
     4081 gtgcttgacg gggagcttga cgacctcagc cgggctgtga cccgtgctcg gctggaactc
     4141 ggtcgtacct ccagcgacgt ccgcccgcga ggccggttgg gctctcgctc ccggcggcaa
     4201 cgctgtatct ctgctcatcc cagtcctttt ccatcgcaac cgcagcgcga aacagcatcc
     4261 gaggatgctt ggtcccgctg tgatccgtcc tgcgaaggcc ccagccctcg attatttgtt
     4321 cacaagaaca cctgaaaccg caatgtacac aagatgttct gtaataccag gagagacttt
     4381 caatctctcc gccgcgccgt gttgcgtacc aaggtttagc gcccgggcac aggtagatca
     4441 agcaccctcg gcgcaaatga catctttgtc attccagtcc tcggggtcag gatcgttccg
     4501 tggtagctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca aatgacatct
     4621 ttgtcattcc agtcctcggg gtcaggatcg ttccgtggta gctctgtcgg cgcgatcccg
     4681 ggtgtccctg agcaaaactt cctgggcgac cgtggcaacg tgccgacccg caaggtcgaa
     4741 aacgtgcccg atcgccagtc cgcggcctcc gacgaagctg tggcacgtga agtcgtggag
     4801 gtgccactca tcggcacgca tcggccggtg gaaccagatc gtgtggtcga ggctggctgt
     4861 gaacaggacg cggtagcgga gttcctcggg ttcggcgccg atgggatggg cgcggacgac
     4921 cgcgtcggtc ggcaggtcgt cggagaggta ggcaagggcg gcgaagttct ggagcgcgtc
     4981 gtcgccgatc ggcttcgacg tacgaagcca cgccgcggcc ctcccggcgc cgagccgcga
     5041 gtcgggttcg agctggtcct ccggcacgaa cgcccgctcg aagaccgggc tccacgacgt
     5101 cgattccagg tgttcggggc ccggcagcgc acggtccatc gtcaccacat cgaggtcggg
     5161 cgactcctcc ttcgcctgga acgacgcctc gaggttgagg atcgccccga tggcctggcg
     5221 cgcgacgacc cgacgggtgg cgaacgaccg gccgttgcgg atgcggtcga cctcgtagcg
     5281 gaccggttcg tcctggtcgc cgcggcggat gaaataggcg cgcagcgaat gcaccacgag
     5341 gtgatcctcg acggtcgcgg ccgctgcctc gagcgcctgc gccacgatct ggccgccgta
     5401 gagacctccc caggggtaac ggggccccga gcccacgaac gtgtcggggc cgtgagagga
     5461 cagggtcagg atgtcggcga gctccatcgg cccattctgc cccacccgtt ttgccctgca
     5521 ttccaatcgg cagaccgatc gttctgcagg gcagaacccg ggtgggtcct acgttcgcgc
     5581 tcgtcggacg aactcgtggg agcgaaagtc gcgcatgcgc ttccagtacg agacggtgaa
     5641 cgccggccag ttgttggtga cgaccccgtg ctcgttcttg taccagctgt cgcagtcggc
     5701 cacccacacg gtgccgtcgg cgttgtgctg gatccgggtg ttgtagaggt ccatcacgtc
     5761 gtcgcggacg tcgagccagg ccaggtggtc gtcgcgcagc tcgcggacgg cgcgactgat
     5821 gtaccgcgcc tgcgcctcga tcatgaagat gatcgagttg tgtccgaggt tggtgttcgg
     5881 gccgtagagc atgaagaagt tggggaagcc ggcgacggcg acaccgaggt gcgccttggc
     5941 ccccttcgcc caggccgtgt tgaggtccgc gccgtgtcgg ccggtcacct ccatcggcgc
     6001 caggaactcg gtgctgcgga acccggtccc gaagatgagc acgtcgagct cgtgacgggt
     6061 gccgtcggca gtgaccacgg ctccgggctc gatgtggtcg atgtgggaca gctcgacctc
     6121 gacgttcggg cgcaggagcg aggcgtagta gtcgttcgag atcaggatcc gcttgcagcc
     6181 cggcgggtag tcggggatga gggcctcggc gggaaggtcg tcggagacga tcttgcggag
     6241 ctccttggtg gcgacacgtt cgatgagctt gccgagccgc gagccgcgcc gcatgaagaa
     6301 gaagttcttc tcgaggcgcc agtagatgtt ccaccggaag agccgctcgg cgaacggcac
     6361 gttgcggaag atccggcgct cgagcgggga gaactcacgg tcgggcttgg ggaggatcca
     6421 gttggcgctg cgctggaaca gcgtcaggtg accggcgacc tcggcggtcg ggggtaggaa
     6481 ctgcaccgtg ctcgccccgt tgccgatgac gccgacgcgg cggccggcga ggtcggtgtc
     6541 gtggttccaa cgcgccgagt ggaacacgtc gccgccgaac tcggcgaggc cctcgatctc
     6601 gggggtgaac ggacgattga gctgaccgag accggagatg agcacgtcgg cggtgagcga
     6661 cgtgccgtcg gcgacggtga cgtgccactc ccccgcgtcc tcgtcgaacc gggccgaggt
     6721 ggcctcggtc ccgaagcgga tccgatggcg caggtcgtag cggtcggtca cgtcctcgaa
     6781 gtacgagagg atctccggct gctgcgcgaa cttgcgcgtc cagtccttct tcggggcgaa
     6841 cgagaacgag tacaggtgcg acggcacgtc gcacgccgcg cccgggtagg tattggcacg
     6901 ccacgtgccg ccgacgccgt cggacttctc gaggatcgtc aggtcg
//
LOCUS       DAOSOA010000163         6902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11042_length_6902_cov_11.082518, whole genome shotgun
            sequence.
ACCESSION   DAOSOA010000163 DAOSOA010000000
VERSION     DAOSOA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11042_length_6902_cov_11.082518"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(683..1150)
                     /gene="ssb"
                     /locus_tag="PKY13_10600"
     CDS             complement(683..1150)
                     /gene="ssb"
                     /locus_tag="PKY13_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT80429.1"
                     /translation="MSHANTDLDGNVTRDPELRFTPSGQPVATFGLAVNRRWQNRQTQ
                     EWEESVSFFDVVCWGQLAENVAASVTKGDGVIVVGRLDQRSWETQEGDKRSKVEIVAN
                     NVGPSLMWATAEVTKNPRQGGDGAPHPADAASGSASAAAPAATTAPDYDEEPF"
     gene            complement(1359..2060)
                     /locus_tag="PKY13_10605"
     CDS             complement(1359..2060)
                     /locus_tag="PKY13_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOT80430.1"
                     /translation="MNGHDHALFGLAAYQAAVLAGVTTFGQPPPGLATIALGAAVAVG
                     AALIADVDEQHSVSAQGLGVAGAAVRVASSATGGHRTLTHYPLLCVGGLIALCRWLIG
                     AGPGWWFAGLCGVMIAAGTPHLIGKKRRSKAGPFVSVVALGAGALAAWLLHRYNVAPD
                     WWLYAAVPIPYLAHLVGDTPTPAGVPWLHPLSSRKFGLPVFHSGGTFENTVVCPLLIA
                     ANAFALYRLHVANVI"
     gene            2136..2606
                     /locus_tag="PKY13_10610"
     CDS             2136..2606
                     /locus_tag="PKY13_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80431.1"
                     /translation="MADMGGLVERRPNRSRTFRVVAIAAVVVVASVVVYLATRPKPMV
                     EGALLFPALEERGIRCAPMVTPAVSDDDHECTIDGVDQLFRVSPNGVWVEIGPISAAT
                     GSRDLLDALGSVFGWGPSDSQRMIGTTAFDGQEEGGDVVWVFDGEGVVIEAPPP"
     gene            complement(2614..3237)
                     /locus_tag="PKY13_10615"
     CDS             complement(2614..3237)
                     /locus_tag="PKY13_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80432.1"
                     /translation="MARGEPFANHHLRSEGDRVCAELARRSIAHGLSSAVKISTIFPR
                     EMADTALVDIFVEARPDIYYSLYSVNPSFRRRWLPRALPTDVALQALREWQLATTLTP
                     MLHWALIAGENDSPADVDAICQAVTDAGLRCNINLVRYNPFSERHGSEPAIETIDQVA
                     ARLLDGLPGTTVTIVSRVGMDVAASCGMFVDDASGQPVVVSGRKAKS"
     gene            complement(3464..4762)
                     /locus_tag="PKY13_10620"
     CDS             complement(3464..4762)
                     /locus_tag="PKY13_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT80433.1"
                     /translation="MTDRRTFVLDTSVLLADPHAIDRFDEHHVVVPVAVITELEQKRT
                     HPELGWAAREALRRLEQIRVEFGELHTANRNGKGTVRVEMNNVGTANLPEALRTDSAD
                     TRVLAVARNLGTRVDCDVVLVTKDLPLRLRAALADVTAEEYQYDQVTQQWTGMIEVTD
                     PSLMDELQDNGDAPADSIPDPVANCGVIVRGDGRSRSTIGRIVFAGPQDKAPTVQRLA
                     NRQLFGLRPKSAPQRVAVDLLTDPTVGIVSIGGKAGTGKSVLALAAGLDAVLEQETHR
                     RVLVFRPIQPVGGQDLGYLPGSEAEKMGPWAGAVDDALEAICGPEVRAEVQRRGLLEV
                     LPLTHIRGRTLTDSFVVIDEAQNLERMVLVTALTRIGSGSRVVLTHDVDQRDNPHVGP
                     NDGVAAVIAALKGSHLFGHVTLTKTERSPVAALVANAFGG"
     gene            complement(4759..5121)
                     /locus_tag="PKY13_10625"
     CDS             complement(4759..5121)
                     /locus_tag="PKY13_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80434.1"
                     /translation="MDQRPTPIHQPHDGETERVDQPPAPIDEAIGAAAAGATDDLDED
                     DPDLDARIATIVRSHLAKLDPLDPQLTDKCAAILAEVIVTSMATGLRFKVPLVADMRA
                     HVFQVAEVRDILARRAAA"
     gene            complement(5190..5519)
                     /locus_tag="PKY13_10630"
     CDS             complement(5190..5519)
                     /locus_tag="PKY13_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT80435.1"
                     /translation="MPHRIDDQWELSAACADSGAESARLFFSDDPTEIATAKQVCALC
                     PVITECLDTAIENREQWGVWGGQLFIDGRIASIKRRPGRPPAVPNPDDEIPMLPVPDR
                     HRHLLSQ"
     gene            complement(5588..>6902)
                     /locus_tag="PKY13_10635"
     CDS             complement(5588..>6902)
                     /locus_tag="PKY13_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80436.1"
                     /translation="TTTTAPPTTTTTSTTVPPTTTTTTTAPPSTTTTSTTAPTTTTTT
                     AAPSDLCAELTAKQALNPPKYDRNTYWNRPAACFQTRPDSAQWAYRWWNFANVPGRTN
                     PAHRGEIAVSFDEYSTPVYDKADATGTARVFTTGWGYGHNLGADNSLPWNPSWEPAPG
                     NDAELIIKEPSTGREWGLWLLQKINWSSCLTLENLFAGYQPGVDLCAGAASIGRNPDG
                     TVSNYFTDSGFADPSTGRGMGAIQGMALLPTLDEIENGSINHALNMETYATMFGPACT
                     AAQLGTPAAGVDCGFAVTPATRLEFWQGPATAECGPVAQQNTSAGRSMTVPEGMRFVI
                     DLSDAEIETWLDGRGYTGAKRNTARIFAVALRDYGWIISDTTCWTSATAVEGVANPQA
                     RARWAALGVTNITPQANSEFLQGLVPAESKVRTIEGPDGVIRTNP"
BASE COUNT          950 a         2014 c         2685 g         1253 t
ORIGIN      
        1 tgggtcacgt gcacgcgcct tgggacgtga acgagcttgc gacggttcct cccgtcgcgg
       61 tgaacgttgc cgtgtagacc ccggcaggac cggtgagggt gaccgtggcc gagccgctgc
      121 ccgtgctctg gcctgttgct gggccggtga catcgagagt gaccgtgccc ggcccagtcg
      181 cggagatggt gagtgtcgcg tcggtggtgc aggtgatggc ctgtgtcccg cgagctggtg
      241 ttggggtcga acgccgcgtt gtcggcggag cggttggcgc gaccgtgggc gccggctgcg
      301 ttgggggtgc ggtggtggca gcggtggtgg cgggcggggc cgttgttggg ggtgcggtgg
      361 gttcgggtgc ggggagcggt ggaatcgtgg tgctcggtgg cgcacttgtt gttgtggcga
      421 gagggtcgtt cacgagggag gtggacgagc tggcaggggt gtcggttggg acgctgtcgg
      481 gtgtcgatgg gagcgccgcc aacgcgagaa caccgatcgc cgcgatgagg gtcgcagcga
      541 tcggtgttct cagtcgccag acgtaccagc gtgcggtatc ccgattcgtt cggagcagag
      601 gtgtgtcggc ggccatggtt ggtgaagggt tccgggggcc acggcaggat cagaccgtgg
      661 cccccggaag ggggtatccc gactagaacg gctcctcgtc gtagtcggga gcggtcgtcg
      721 cagcgggagc tgcggccgaa gccgacccgc tcgcagcgtc agccggatgg ggcgcaccgt
      781 cgccgccctg acgggggttc ttcgtgacct cggcggtcgc ccacatgagg ctcgggccga
      841 cgttgttggc gacgatctcg accttggagc gcttgtcgcc ctcctgggtc tcccacgatc
      901 gctggtcgag acggccgacg acgatgacgc cgtcgccctt ggtgacgctc gcggcgacgt
      961 tctcggcgag ctggccccag cagacgacgt cgaagaagga gacggactcc tcccactcct
     1021 gggtctgacg gttctgccag cgccggttga cggcgagacc gaacgtggcc accggctgac
     1081 cgctgggcgt gaaacgcagc tcggggtccc gggtgacgtt gccgtcgagg tcggtgtttg
     1141 cgtgtgacat gggtggttct cctcgcccgg gtttcgggct tctctgttgc cgccttgtgg
     1201 cggcgtgggt tcctatggac gtcaccaatc cgctatgggc ggactgggac gggggccgac
     1261 gctgtgctcc tttcgagtgg cggctgggtg gtggcgtcga tgcctgtggg gtatgtgggc
     1321 cgggttgggg tggatcccgc ccaaggtcgc cgcgggacct agatcacgtt ggcgacgtga
     1381 agccggtaga gggcgaatgc gttggcagcg atgagcagcg ggcagaccac cgtgttctcg
     1441 aaggtcccgc cggagtggaa gacggggagc ccgaacttcc ggctcgagag cggatggagc
     1501 caggggaccc cggcgggggt tggggtgtca ccgaccagat gtgcgaggta cgggattggt
     1561 acggctgcgt agagccacca gtcgggcgcg acgttgtagc ggtggagcag ccatgctgcg
     1621 agggcgccgg cgccgagggc gacgacgctg acgaagggac cggccttgga gcgccgtttc
     1681 ttgccgatca ggtgtggtgt gcctgcggcg atcatcacgc cgcagagccc ggcgaaccac
     1741 cagccgggac ctgcaccgat gagccatcga cagagggcaa tgagaccgcc gacgcagagc
     1801 agcggatagt gggtgagggt tcgatggccg ccggtcgccg acgatgcgac gcggaccgct
     1861 gcgccggcga ctccgaggcc ttgggcgctg acggagtgtt gctcgtcgac gtcggcgatc
     1921 agggctgctc cgaccgctac agcggcgccg agagcgatcg tggcgagtcc gggcggtggt
     1981 tgcccgaagg tggtgacgcc ggcgagtacg gcggcctggt aggcggcgag tccgaagagt
     2041 gcgtggtcgt gtccgttcac acctcgaaca tgcgtaggcg ggttccatca cccccccccc
     2101 gggccccgcc gagcgctcgt gcctagattc gaaccatggc cgacatgggc gggttggtgg
     2161 aacggcggcc gaaccgctct cgaacgttcc gggtggtcgc tatcgccgcg gtcgtggtgg
     2221 tcgccagcgt cgtcgtctat ctcgcgacgc ggcccaagcc gatggtcgag ggcgcgctgc
     2281 tgttccctgc gctcgaggag cgcggcatca ggtgtgcacc gatggtgacg ccggccgtgt
     2341 ccgacgacga ccacgagtgc acgatcgacg gtgtcgatca gctgttccgt gtctcgccga
     2401 atggggtgtg ggtcgagatc ggcccgatct cagctgcaac aggttctcga gaccttctcg
     2461 atgcgttggg ttcggtgttc ggctggggtc cgtcggactc gcagcggatg atcggcacca
     2521 cggcgttcga tggccaggaa gagggcggcg atgtcgtgtg ggtgttcgac ggcgaaggcg
     2581 tcgtgatcga ggccccgcca ccctgaccgc tacctaggac ttcgccttgc gaccactgac
     2641 gacgacgggt tggccgctgg cgtcgtccac gaacatgccg cacgaggccg cgacatccat
     2701 tccgacgcgg ctcacgatcg tcaccgtcgt cccgggcaac ccgtcgagca gccgagcggc
     2761 aacctgatcg atcgtttcga tggccggctc ggagccgtga cgttccgaga aggggttgta
     2821 acgcacgagg ttgatgttgc atcgcagtcc ggcgtcggtg accgcctgac agatcgcgtc
     2881 tacgtcggcc ggcgagtcgt tctcgccggc gatgagcgcc cagtgcaaca tcggcgtgag
     2941 tgtggtggcg agctgccact ccctgagcgc ttggagggcg acgtcggtgg gcagggcgcg
     3001 aggcagccat cgacgccgga acgacgggtt caccgagtag agggagtagt agatgtccgg
     3061 ccgggcctcg acgaagatgt cgacgagtgc ggtgtcggcc atctctcggg ggaagatcgt
     3121 cgagatcttg actgcggagc tgagtccgtg ggcgatgctg cgacgggcga gctcggcgca
     3181 gacgcggtcg ccctccgagc gaagatggtg gttcgcgaag ggctcacctc gcgccatgaa
     3241 gttgaaatga accttggcgg agggccgacc cctcgggtct gcccgctctg ggtgaggtga
     3301 cagaagcggc agcccttgtt gcaccccgtt tgcgagctca cgtacgcgat gaagtagtca
     3361 tcgcggcgtc gcacgaaccg cgcctcgacg attccgtcag cagtgtcggc tacgaagttg
     3421 accgagaggt cctgcctgga gacgagccga gtggacgcca cggtcagcct ccgaacgcgt
     3481 tggcgaccag cgcggcgaca ggggagcgtt cggtcttggt gagggtgacg tggccgaaca
     3541 ggtggctgcc cttgagcgcc gcgatgactg cggccacccc gtcgttcggt ccgacgtggg
     3601 gattgtcgcg ctggtcgacg tcgtgggtga ggacgacacg gctgccgctc ccgatgcgag
     3661 tgagggcggt gaccagaacc atgcgctcga ggttctgtgc ctcgtcgatc acgacgaacg
     3721 aatctgtgag ggtacggccg cgtatgtggg tgagcgggag gacttcgagg agcccgcgcc
     3781 gctgcacttc tgcgcggacc tcgggcccgc agattgcttc gagggcgtcg tcgacggcgc
     3841 ctgcccacgg gcccatcttc tcggcctcgg agccggggag gtagccgagg tcctgaccgc
     3901 cgaccggctg gatcggtcgg aacaccagga cgcgccgatg ggtttcctgc tcgagcacag
     3961 cgtcgagccc ggcagcgagg gcgaggaccg acttgccggt gcccgccttg ccgccgattg
     4021 acacgatgcc gacggtgggg tcggtgagga ggtcgacggc gactctctgg ggcgcagatt
     4081 tgggcctgag gccgaacagt tgacggttgg ccaaccgctg cacggtcggc gccttgtcct
     4141 ggggcccggc gaacacgatc cggccgattg tcgaacgtga ccgcccgtcg cctcggacga
     4201 tcacgccgca gttggcgacc ggatcgggga tcgagtcggc cggagcgtcg ccgttgtcct
     4261 ggagctcatc catcagcgac gggtcggtca cctcgatcat cccggtccac tgctgagtga
     4321 cctggtcgta ctggtactcc tcggcggtca cgtcggcgag cgcggcgcga aggcggagag
     4381 ggaggtcttt ggtgacgagc acgacgtcgc agtcgactcg cgttccgagg ttgcgggcca
     4441 cggcgagcac cctggtgtcg gcgctgtcgg ttcgcagcgc ttcgggcagg ttcgctgtgc
     4501 cgacattgtt catctcgacg cgcacagtgc ccttgccgtt gcgattcgcg gtgtggagct
     4561 ctccgaactc gactcggatc tgctcgaggc ggcggagcgc ctcgcgtgcg gcccagccga
     4621 gctcgggatg ggtgcgcttc tgctcgagtt cggtgatgac ggcgacgggc acgacgacgt
     4681 ggtgttcgtc gaagcggtcg atggcgtgag ggtcggcgag caggacgctg gtgtcgagta
     4741 cgaaggttcg ccgatcggtc atgcggctgc tcgccgggcc aggatgtcgc ggacctcggc
     4801 cacttggaac acgtgggccc tcatgtcggc gaccaagggg accttgaagc ggaggcccgt
     4861 ggccatcgac gtgacgatga cctcggcgag gatcgctgcg cacttgtcgg tgagctgcgg
     4921 gtcgagtggg tccagcttgg cgagatgtga gcgcacgatc gtcgcgatgc gagcgtccag
     4981 gtctgggtcg tcctcgtcga gatcgtcggt tgcgccggcc gcggcggcac cgatggcttc
     5041 gtcgatcggt gccggcggtt ggtcgactcg ttcggtctcg ccgtcgtgcg gttggtggat
     5101 tggggtcgga cgctggtcca tgggcacctg gcctttcggt ggctcgtcgg gttgccctat
     5161 gtggcgccgg cgtggctatg ggcgcgtcgt cactgcgaga ggaggtgccg gtgacggtcg
     5221 ggcacgggca gcatcggaat ctcgtcgtcg gggttgggca ccgctggcgg tcggcctggc
     5281 cgacgcttga tcgaggcgat gcgtccgtcg atgaacagct gtccgcccca cacaccccac
     5341 tgctcacggt tctcgatggc ggtgtcgagg cactcggtga tcacggggca gagcgcgcag
     5401 acctgtttcg ccgtggcgat ttcagtgggg tcgtcggaga agaacagtcg cgcggactcg
     5461 gctccgctgt cggcgcacgc tgcgctgagt tcccactggt cgtcgatgcg atgaggcata
     5521 gaacacatgt gggtcggtag ccgccggccc ccgcccgatg gaggggaggg gggcgggggc
     5581 cggcggtcta ggggttggtg cggatgacgc cgtcggggcc ttcgatcgtg cgcaccttcg
     5641 attcggctgg gacgaggcct tggaggaact cgctgttggc ctggggtgtg atgttggtga
     5701 cgccgagggc ggcccaacga gctcgagcct gggggttggc gacaccttcc accgcggtgg
     5761 cgctcgtcca gcaggtggtg tcggagatga tccagccgta gtcgcggagc gccacggcga
     5821 agatgcgggc cgtgttccgt ttcgctccgg tgtatccacg cccgtcgagc caggtctcga
     5881 tctcggcgtc ggagaggtcg atgacgaagc gcatcccttc gggaacggtc atcgagcggc
     5941 cagccgaagt gttctgttgg gcgacgggac cgcactcggc ggtggctgga ccttgccaga
     6001 actcgaggcg ggttgcgggc gtgacggcga acccgcagtc gacgccggca gcgggcgttc
     6061 cgagctgggc ggccgtgcag gcggggccga acatggtcgc gtaggtctcc atgttgaggg
     6121 cgtggttgat ggagccgttc tcgatttcat cgagcgtcgg caggagcgcc attccctgga
     6181 ttgcccccat gccacgaccg gtgctggggt cggcgaatcc gctgtcggtg aagtagttgc
     6241 tgaccgtccc gtcggggttg cgcccgatgc tcgctgctcc ggcgcagagg tcgactcctg
     6301 gttggtagcc ggcgaacagg ttctccagcg tgaggcagga gctccagttg atcttctgga
     6361 ggagccacag tccccattcc cggccggtgg acggttcctt gatgatgagc tcggcgtcgt
     6421 tgccgggggc tggctcccag gaagggttcc agggcagcga gttgtcggcg ccgaggttgt
     6481 ggccgtatcc ccagccggtg gtgaacacgc gggctgtgcc ggtggcgtcc gccttgtcgt
     6541 agacgggggt gctgtactcg tcgaagctga cggcgatctc cccgcggtgg gccgggttcg
     6601 ttcggccggg gacgtttgcg aagttccacc accggtaggc ccactgcgcg gagtcgggtc
     6661 gggtctggaa gcaggcggcg ggccggttcc agtaggtgtt gcggtcgtac ttcggcgggt
     6721 tgagggcctg cttcgccgtg agctcggcgc agagatcgct tggcgcagcg gtggtcgtcg
     6781 tggtggtcgg cgccgtcgtg gaggtcgtcg tcgtcgacgg cggggcggtg gtcgtagtcg
     6841 tcgtggtcgg cggcaccgtc gtggaggtgg tcgtcgtggt cggcggggcg gtggtcgtgg
     6901 tg
//
LOCUS       DAOSOA010000164         6896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11055_length_6896_cov_6.737465, whole genome shotgun sequence.
ACCESSION   DAOSOA010000164 DAOSOA010000000
VERSION     DAOSOA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11055_length_6896_cov_6.737465"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..77)
                     /locus_tag="PKY13_10640"
     tRNA            complement(1..77)
                     /locus_tag="PKY13_10640"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Ala,seq:tgc)
     gene            161..559
                     /locus_tag="PKY13_10645"
     CDS             161..559
                     /locus_tag="PKY13_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HOT80437.1"
                     /translation="MTIDADLKALAQGKNFAALTTILPSGHPQTQIMWVGADDEHVLI
                     NTEAGRQKFRNVEADPRVTVCVFNNDNPYQYVEARGKVVGTAAGDEARAHIDELSQKY
                     MGAPYGNPIGPEGRVILKIEVERIHKNGIG"
     gene            complement(592..1794)
                     /locus_tag="PKY13_10650"
     CDS             complement(592..1794)
                     /locus_tag="PKY13_10650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT80438.1"
                     /translation="MDDPTERLITPTFIAITLSTFAYFMSLGSLLPTLPRFIEEELGG
                     SSVAVGFGIGTFAIAAAILRPFAGRLGDLRGRRILIVGGSFLVGISVLGYTFVDSLTT
                     LLVLRFVTGVGEAAMWVGAATAIQDMAPEARRGEAASYFSVALYAGLAFGPLVGEALV
                     DGHDYQPLFIASGISALIASALGFFTPSAAPADPQPFRLFHPAAVAPGGILLLGLLPF
                     VGFSTYVSLYGPKIGIDDVAPLLLLYGGLVLVIRIVAAKAPDRLGWRRASTIALCLLG
                     GAGAIMAAWGAAPGLWIATFGLALGMSLLFPALMSAVVADVPENERGQAVGTFSLAFD
                     LANGLGPVLLGAVVAISSYRPAFSVAAVGAFAGLLLVSRMAGQTAALTSAGIPHGSTV
                     EDSVHPPS"
     gene            complement(1897..2916)
                     /locus_tag="PKY13_10655"
     CDS             complement(1897..2916)
                     /locus_tag="PKY13_10655"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HOT80439.1"
                     /translation="MSGGRSRTRLLALIALPTVLLAACGSGKEASDSTTTTKGAPTTS
                     TATTTPAKAAGSSAGAPTSARAPEVGDCLEPMTQPIAGSHQLPAIVPCDGPHGGEVVS
                     VLEIPAPTDDTYPGWGRNLEGADEQVSACEGDGDAPGQLTEFLGSAPLTLTDAQVAKG
                     AFGAYAVTGIQYATFIPGPAAWQAGERWLACSAVLSNRLKVPSSYTGTLKGALAKPGS
                     LDEQFSWCKQQPPDSRDTFTVVPCSEPHNYEQLASYSAGSNDTQYPGDEALGALAEEL
                     CPALSSQATGKRSDDLPDGIGLGWTYPLEYEWSNGDRAVRCFAVTEDGESTGGIGMGT
                     AKLAS"
     gene            complement(3019..3414)
                     /locus_tag="PKY13_10660"
     CDS             complement(3019..3414)
                     /locus_tag="PKY13_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80440.1"
                     /translation="MSDDGFTPPSRERFRQERRAAQAAKRRSRMVGVPAVFLGIGLVG
                     AGMYMWYENDVSDSPVDVQGTSVERTTTSTTTSTTTSTTTTTSTTTTTLPPTTLAPTT
                     TAKPKPKPKPTTSVAPVETTAATVPVTPT"
     gene            complement(3609..5192)
                     /locus_tag="PKY13_10665"
     CDS             complement(3609..5192)
                     /locus_tag="PKY13_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOT80441.1"
                     /translation="MADILTESKRRRTFAIISHPDAGKTTLTEKFLLYGGALTQDAGA
                     VKARADRRSATSDWMALEQQRGISITSTVLQFPYRDCVVNLLDTPGHRDFSEDTYRVL
                     TAADAAVMVLDAAKGIEPQTLKLFEVCRDRGLPLLTFINKWDRPGRDGLELLDEIESM
                     IGVQPTPVTWPVGFAGDFRGVVDRRTGEYTRFTRTARGSKIAPEEVVDAEHAASEEGE
                     AWSTAVDELDLLEGQALDMETFLAGKTTPTFVGSAMTNFGVGKLLDGVIDLVPPPGPR
                     PTKAGTIRPLEAPFSGFVFKVAANMDPSHRDRIAFVRVCSGRFERGMVVTHDRTGKPF
                     ATKYAHQVFGQDRETVEEAFPGDVVGLVNAGDVRVGDSLFESEPVTFPQIPSFAPEHF
                     SIARVRDTGRYKQFRKGVAQLDEEGVVQVLRSPDTGDQAPVLAAVGPMQFEVVAYRLE
                     HEFGAPIELAPTAYRVCRRTDEEGRERLKAMRGVDVLERSDGTLLALFESTYWLDRLI
                     SEEPDLCLEKLVAEGDLHY"
     gene            complement(5193..6374)
                     /locus_tag="PKY13_10670"
     CDS             complement(5193..6374)
                     /locus_tag="PKY13_10670"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT80442.1"
                     /translation="MAEVTGFAAPGFERVRDAFAGNFDDRGDVGAAVALYRDGELVVD
                     LHGGVTEAGGSTPYGPDHLQLVFSTTKGATAICAHILADRGLLDFDAPVSEYWPEFKA
                     HGKADAPVAWLLSHRMGLVDVDRPMTFEQAIAWDPVVEALADSVPLWEPGTDRGYHAV
                     TYGWLVGEVIRRVSGKSVGQFFSDEVAGPLGLDFWIGLPAEQFDRVVPLIPLGPPPGV
                     HFGDPDAPQPGMIEMLQMLMGADSLIARALAAPGGAFTEQDAWNTPEVWQAEIPAANG
                     ITNAPSLARMYAATVGDVEGVRLFGEDTMRNAIEIRSSGPDAVLVFDIPFGLGFMRDS
                     AFAKFGSPSAFGHYGAGGSVGFADHEHGIGFGYVMNKMDLGIAGDPRTAALIDAVYAS
                     L"
     gene            complement(6491..>6896)
                     /locus_tag="PKY13_10675"
     CDS             complement(6491..>6896)
                     /locus_tag="PKY13_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80443.1"
                     /translation="TTIAGQPAPTTTTTTTTTIAGQPAPTTTTPPITATGLFSSVYSD
                     HREPTPLDKSRAGARVWIFYDGPGVTSVTYWVDDPTGSGRPTRVAEGPFDLDPAGTDW
                     RVQYGLGSHTVLAEVTTVDGTFRRLATFDVYR"
BASE COUNT         1130 a         2359 c         2411 g          996 t
ORIGIN      
        1 tggtggagga gatgggactc gaacccacga ccccctgctt gcaaagcagg tgctctagcc
       61 aactgagcta cacccccgcg aacgggcatc ggtcatcgta tcgggtcatc ggcaaccacc
      121 gcgaccgaga tcccctccga gcggcagtag cttcggtcac atgacgatcg acgcagacct
      181 caaggcgctc gcgcagggca agaatttcgc cgcgctgacc accatcctcc cgagcggcca
      241 cccgcagacc cagatcatgt gggtcggcgc cgacgacgag cacgtcctca tcaacaccga
      301 ggccggtcgt cagaagttcc gcaacgtcga ggccgacccg cgggtcaccg tctgcgtctt
      361 caacaacgac aacccgtacc agtacgtcga ggcccgcggg aaggtcgttg gcacggccgc
      421 tggcgacgag gcccgcgccc acatcgacga gctctcccag aagtacatgg gtgcgcccta
      481 cggcaacccg atcggccccg agggtcgtgt gatcctcaag atcgaggtcg agcgcatcca
      541 caagaacggg atcggctgac gctgaccctc cacggtcgaa ccgtggcgag atcagctcgg
      601 ggggtggacg gaatcctcca ccgttgaacc gtgggggatt cccgcggacg tgagcgcggc
      661 ggtctggccc gccatccggc tcacgaggag cagcccggcg aacgcaccga ctgcggcgac
      721 ggaaaaagcc gggcggtacg acgagatcgc aacgacggca ccgagcagca ccgggcccag
      781 gccattggcc aggtcgaacg cgagcgagaa cgtgccgacg gcctggcccc gctcgttctc
      841 cggcacgtcc gccacgacgg cggacatgag ggctgggaac agcaacgaca tgccgagcgc
      901 gaggccgaac gtggcgatcc acaaccccgg cgcggcaccc cacgccgcca tgatcgcccc
      961 ggcaccgccg agcaggcaca gcgcgatcgt cgacgcccgc cgccaaccga gccggtcggg
     1021 tgccttggcc gcgacgatgc ggatcacgag caccagcccg ccgtacaaca agagcagggg
     1081 agcgacgtcg tcgatgccga tcttcgggcc gtacaacgag acgtaggtgc tgaacccgac
     1141 gaaggggagc aggcccagga gcaggatgcc tcccggcgcg acggccgcag ggtggaacag
     1201 ccggaatggc tgggggtcgg ccggcgcagc cgacggggtg aagaacccga gtgcgctggc
     1261 gatgagtgcc gagatgccgg acgcgatgaa caacggctga tagtcgtgcc cgtcgaccag
     1321 tgcttcgccg acgagcgggc cgaacgccag accggcgtac agggcgaccg agaagtacga
     1381 cgctgcctca ccacgacgag cttcgggtgc catgtcctgg atggccgtcg ccgcgcccac
     1441 ccacatcgcc gcctcgccga ccccggtcac gaaccgcagc acgaggagcg tcgtgagcga
     1501 gtcgacgaag gtgtacccca acaccgagat gccgacgagg aacgagcctc cgacgatgag
     1561 gatgcgccgg cctcgcaggt cgccgagccg accggcgaac ggccgcagga tcgcggcggc
     1621 aatcgcgaac gtaccgatgc cgaacccgac ggcgacactg cttccgccga gctcctcctc
     1681 gatgaaccgg ggcagtgtcg ggagcagcga cccgagcgac atgaagtacg cgaacgtcga
     1741 cagggtgatc gcgatgaacg tcggggtgat cagtcgctcg gtcgggtcgt ccatccgggg
     1801 caacaacgtc gagggtcgtc ggcgaattcc gccgggccgg agtcggcccc ccgatcgggg
     1861 agccgagcgg tctcccgatc agggagcaaa tcgccgtcag ctcgcgagct ttgccgtgcc
     1921 cattccgatg ccgcccgtgc tctcgccgtc ctcggtgacg gcgaagcagc gcacggcgcg
     1981 gtcgccgttc gaccactcgt actcgagggg gtacgtccag ccgagtccga tgccgtcggg
     2041 tagatcgtca ctgcgtttgc cggtcgcctg ggagctgagc gccgggcaca gctcctcggc
     2101 cagggcgccg agtgcctcgt cgccggggta ctgcgtgtcg ttggaccccg ccgagtacga
     2161 cgccaactgc tcgtagttgt ggggttcgct gcacggtacg acggtgaacg tgtcccgcga
     2221 gtccggcggc tgctgtttgc accagctgaa ctgctcgtcg agcgaaccgg gcttcgccaa
     2281 cgcgcccttg agcgtgccgg tgtagctcga cggaaccttg aggcggttgc tgagcacggc
     2341 cgaacaggcc agccagcgtt cgccggcctg ccacgcggcc gggccgggga tgaacgtcgc
     2401 gtactggatg ccggtcaccg cgtaggcccc gaacgcgccc ttggccacct gagcgtccgt
     2461 caacgtcagc ggggccgacc cgaggaactc ggtcagctgg ccgggagcgt cgccatcccc
     2521 ctcgcaggcg ctgacctgct cgtcggcccc ctcgaggttg cgaccccagc cggggtacgt
     2581 gtcgtcggtc ggcgccggga tctcgagcac ggagacgacc tcgccaccgt ggggcccgtc
     2641 gcacggcacg atggccggca gctggtggct ccctgcgatc ggctgggtca tcggctcgag
     2701 gcagtcgccg acctcaggag cgcgcgccga cgtcggagcg cccgcggatg aaccggccgc
     2761 cttggccggc gttgtcgtgg cggtcgacgt ggtcggtgca cccttggtcg tggtggtgga
     2821 gtcggacgcc tccttgcccg aaccacacgc agccagcagc accgtcggaa gcgcgatcag
     2881 cgccaggagg cgggtccggg accggccgcc gctcacgcgg cgacgatcag gaacggcaca
     2941 tcttcgccaa ggcggcggtc acgcggaacg ggcatccgcc gagggtagcg gagcgtcggc
     3001 gagacccgtc ggggaacgtc aggtcggtgt gacgggcacg gtcgcggcgg tcgtctccac
     3061 cggtgcgacc gacgtggtcg gcttgggttt cggcttcggt ttggccgtgg tggtgggcgc
     3121 gagggtggtc ggcggaagcg tggtggtcgt ggtcgacgtc gtggtggtgg ttgacgtcgt
     3181 cgtcgaggtg gtcgtcgacg tcgtggtgcg ctcgacgctc gtgccctgca cgtcgacggg
     3241 cgaatcgctc acgtcgttct cgtaccacat gtacatgccg gcaccgacga gcccgatgcc
     3301 caggaacacg gccgggacgc cgaccatccg cgagcggcgc ttcgctgcct gtgctgctcg
     3361 gcgttcttgc cggaaccgtt cccggctcgg cggtgtgaag ccatcgtcgc tcacggacag
     3421 cccctccttc atcccagacc gccgccaaaa ctagttcggc ccggccgggg ttccgcggac
     3481 ccaggggtga tggtggtcgc gatcacgcgg cggtcgacga agtcgggccg ccgcccggaa
     3541 ggacagttcg ttcgtcggcg gagccgacag aattccggag ccaccgaggt gaagctggcc
     3601 gaaaaccgtc agtagtggag gtcgccctcg gccaccagtt tctcgaggca caggtcgggt
     3661 tcctcggaga tgaggcggtc gagccagtag gtgctctcga acagggcgag cagcgtgccg
     3721 tcggatcgct ccagcacgtc aacgccccgc atcgccttca ggcgctcgcg gccctcctcg
     3781 tcggtgcgcc gacagacccg gtacgccgtc ggggccagct cgatcggcgc cccgaactcg
     3841 tgctccagcc ggtacgcgac cacctcgaac tgcatcgggc ccaccgcagc gagcacgggt
     3901 gcctggtcgc cggtgtcggg agagcggagc acctgcacca ctccctcctc gtcgagttgg
     3961 gcgacacctt tgcggaactg cttgtagcga ccggtgtcgc gcacccgagc gatcgagaag
     4021 tgctcggggg cgaagctcgg gatctggggg aacgtgacgg gttcggactc gaacaacgaa
     4081 tcgccgaccc ggacgtctcc ggcgttcacc aggccgacga cgtcgccggg gaacgcctcc
     4141 tcgaccgtct cgcggtcctg gccgaacacc tggtgggcgt acttcgtcgc gaacggcttg
     4201 ccggtgcggt cgtgggtcac gaccatgccc cgctcgaacc ggcccgaaca cacccggacg
     4261 aacgcgatcc ggtcacggtg cgacggatcc atgttggccg cgaccttgaa cacgaagccc
     4321 gagaacggcg cctcgagcgg ccggatcgtg ccggccttcg tcgggcgcgg gccgggcggg
     4381 gggaccagat cgatcacacc gtcgagcagc ttgccgacac cgaagttggt catggccgac
     4441 cccacgaacg tcggcgtggt cttacccgcc aggaaggtct ccatgtcgag cgcctggccc
     4501 tcgagcagat cgagctcgtc gaccgcggtc gaccaggctt ccccctcctc ggacgcggcg
     4561 tgctccgcgt cgacgacctc ctcgggagcg atcttcgaac cacgggcggt ccgggtgaac
     4621 cgcgtgtact cgccggtccg acgatcgacc acaccccgga agtcgccggc gaatcccacc
     4681 ggccacgtga ccggtgtcgg ctgcacgccg atcatcgact cgatctcgtc gaggagctcc
     4741 aggccgtcac ggccgggacg gtcccacttg ttgatgaacg tgaggagcgg caggccccgg
     4801 tcgcggcaga cctcgaacaa cttgagggtc tgcggctcga tgccctttgc agcgtcgagc
     4861 accatcaccg cggcgtcggc ggcggtcagc acccggtacg tgtcctcgga gaagtcccgg
     4921 tgacccggcg tgtccagcag gttcacgaca cagtcgcggt aggggaactg cagcaccgtc
     4981 gacgtgatcg agatgccacg ctgttgctcg agcgccatcc agtccgacgt tgccgatcgg
     5041 cgatcggcgc gggccttcac tgcgcccgcg tcctgggtga gcgcgccgcc gtacagcagg
     5101 aacttctcgg tcagggtcgt cttgccggcg tcgggatgac tgatgatggc gaacgtccgt
     5161 cgccgcttgg actcggtgag gatgtcggcc atctacagcg acgcgtagac ggcgtcgatc
     5221 agcgccgcgg tgcggggatc cccggcgatg ccgaggtcca tcttgttcat gacgtagccg
     5281 aagccgatgc cgtgctcgtg gtcggcgaac ccgaccgaac ccccggcgcc gtagtggccg
     5341 aacgccgacg gcgaaccgaa cttcgcgaag gcggagtcgc gcatgaagcc cagcccgaac
     5401 gggatgtcga acacgagcac cgcgtcgggc ccggagcttc ggatctcgat ggcgttgcgc
     5461 atggtgtcct cgccgaagag ccggacgccc tcgacgtcgc ccaccgtcgc tgcgtacatc
     5521 cgggccaacg acggtgcgtt ggtgatgccg ttggccgccg ggatctcggc ctgccacacc
     5581 tcaggggtgt tccacgcgtc ctgctcggtg aaggcgccac ccggagcggc gagcgcccga
     5641 gcgatgaggc tgtcggcgcc catgagcatc tgcagcatct cgatcatgcc tggctgcggg
     5701 gcatcgggat caccgaaatg cacaccgggc ggcggcccga gcgggatgag tgggaccacg
     5761 cggtcgaact gctcggcggg caggccgatc cagaagtcga ggccgagcgg ccccgccacc
     5821 tcgtcggaga agaactggcc gaccgacttg ccgctgaccc ggcggatgac ctcgcccacc
     5881 agccacccgt aggtcacggc gtgatagccg cggtccgtgc cgggctccca cagcggaacc
     5941 gaatcggcca acgcctcgac gaccggatcc caggcgatcg cctgctcgaa cgtcatcgga
     6001 cggtcgacgt cgacaagacc catccggtgc gacagcagcc aggccacggg agcatcggcc
     6061 ttgccgtgcg ccttgaactc cggccagtac tccgacaccg gagcgtcgaa gtccaggagt
     6121 ccacgatcgg ccaggatgtg ggcgcagatg gcggtcgcgc ccttcgtggt ggagaacacc
     6181 agctgcaggt gatcgggacc gtacggggtg gatccgccgg cctccgtgac gccgccgtgc
     6241 aggtcgacga cgagttcacc atcgcggtac agcgcgacgg ccgcgccgac gtcaccgcgg
     6301 tcgtcgaaat tgccggcgaa cgcatcgcgc actcgttcga accccggtgc cgcgaatccg
     6361 gtcacttcgg ccatgtcatc tcctcaggtc ggcgttcgat ccggctcgat ggtacgggcc
     6421 ccaccctgcg gcgtcgatcc gtcgtcagag cccggtcgcg acggccggcc tcgacgtcgt
     6481 ccggtctcgg tcagcgatag acgtcgaagg tggcgagccg ccggaacgtg ccgtcgaccg
     6541 tcgtgacctc ggcgagcacg gtgtgggagc cgagcccgta ctggacccgc cagtcggtac
     6601 cggccggatc gaggtcgaag gggccctcgg cgacccgcgt cggccggccc gagccggtcg
     6661 ggtcatcgac ccagtaggtc accgacgtga ccccgggccc gtcgtagaag atccacaccc
     6721 gagcgccggc ccgactcttg tcgagcggcg tgggctcgcg atggtcggag tacacgctcg
     6781 agaacaggcc cgtggccgtg atcggcggcg tggtggtggt cggagccggc tggccggcaa
     6841 tggtcgttgt ggtcgtcgtc gtggtggtcg gagccggctg gccggcaatg gtcgtt
//
LOCUS       DAOSOA010000165         6892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11064_length_6892_cov_4.896300, whole genome shotgun sequence.
ACCESSION   DAOSOA010000165 DAOSOA010000000
VERSION     DAOSOA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11064_length_6892_cov_4.896300"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(658..1434)
                     /locus_tag="PKY13_10680"
     CDS             complement(658..1434)
                     /locus_tag="PKY13_10680"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOT80444.1"
                     /translation="MGRLDDKIAIITGGGAGMGRIACQRFAAEGAKVVVADNNADGAA
                     ETVEAITAAGGTALPATVDVRDAGQVEAMVKIAVDNWGGLDILYNNAGVSPGDDDTPL
                     NTSEETWQFTFDVNVKGLFLCNKYGIQAMLDSGGGSIVNVASFVAHMGAATPQIAYTS
                     SKGAVLAMTQEIAAIYARQGIRANSLCPGPVLTPLLAKFLSDEEKRQRRLVHIPMGRF
                     GEAEEMVNGALFLASDESSYMTGQSLLIDGGITKTYTTPL"
     gene            1575..3035
                     /locus_tag="PKY13_10685"
     CDS             1575..3035
                     /locus_tag="PKY13_10685"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT80445.1"
                     /translation="MSSAPLHQPLASSYDLVIAGEKVPAAEGTTFTAYEPSTGAALAT
                     VAQAGSVDVDRALNAAVAEFEEGTWRTTNATARGKVLLKVSQLLRERAEDFAIAESRN
                     SGHPIGNSRWEANAAADVFEYFAGAANKHYGQTIPVQDNGLDVVLREPVGVCGLIVPW
                     NFPLLITTWKVAPALACGNPVVIKPATLTPITALMLADVLLDAGVPGGCISVIPGPGA
                     SVGDKLAGDPRVAKLSFTGDSSTGAQIMRRSSDNITRLSLELGGKSACIVFEDADIEA
                     AIAATPMSVFDNTGQDCCARSRFIVHRSVYDQFVEGFVKVTEGLVTGDPLDESTQLGP
                     MISEGQRQTSLDYLAIGAAEGANRLCGGEESGPGWYLTPAVLADVDNSMRVAQEEIFG
                     PVASMIPFDTEADAIRIANDSPYGLSGSLWTNDLGRSIRVTRAVRTGVISVNSTRSVR
                     TEAPFGGFKRSGMGRELGMAAMDHYTEVKNVFYSQD"
     gene            3043..3909
                     /locus_tag="PKY13_10690"
     CDS             3043..3909
                     /locus_tag="PKY13_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80446.1"
                     /translation="MSRSMMTLPTAADPASVVDTDAYPFGDSDCYPWILAARADLAAD
                     GVAILPGFVRPEAVATMCVEADRLAPLGHHSVVQGTPYLGLPDESFPEGHPRRALVDN
                     SLTAVAYDLIGADSLLRVLYEWPRFTEMIRRMLDRPALYHYADPFGALNIAAMSAGDE
                     LGWHFDQTDFVVSIALQSSSGGGDFESVARLRSAEDDAYDEVSAVLAGDRSRVSSVPM
                     TPGTLMLFEGRNSLHRVTKIEGDVPRHVALLAYDTVPGTDSSELLKLVRYGRSPETVA
                     GPGVRSDGGQQQ"
     gene            3929..4441
                     /gene="fae"
                     /locus_tag="PKY13_10695"
     CDS             3929..4441
                     /gene="fae"
                     /locus_tag="PKY13_10695"
                     /inference="COORDINATES: protein motif:HMM:NF020297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HOT80447.1"
                     /translation="MSWTGMLIGEGFEGSGTSAAHINAVLGDRTSPVGQAWVQALATP
                     SAGHAPFMVVARPGLPVKPFTLFVSKATTSNRDHADVTWGAAQAGVAAGVVEAVSAGI
                     IPTDVAEEALLICAVWVDPGATVAHSDVVFLNNRVATYAALENAGHHRPSVADVLSDD
                     QGIWNPFYRP"
     assembly_gap    4536..4635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4774..5049
                     /locus_tag="PKY13_10700"
     CDS             4774..5049
                     /locus_tag="PKY13_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80448.1"
                     /translation="MTGPRKKICALVLAASVSVGLAGCSRPDANAPVRITGDKDAAIP
                     ATDPTTTTSTTEAPPTTLFYSPDGPGSKIAAETTTTTEAEPTTTEAE"
     gene            5085..5717
                     /locus_tag="PKY13_10705"
     CDS             5085..5717
                     /locus_tag="PKY13_10705"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HOT80449.1"
                     /translation="MEPLTGRPLLGTPVPPPYQAFELHPNAFTPRQCDRIVALGEQLR
                     AESAGLEGTDGDEVNDESIRRSRTAWIAPDDDSWWVYDKLAKIAERANRRYGFDLTGF
                     GEDLQFTFYDEPGAFYSWHQDGLDGGVATRKLSIVIQLTDPEDYEGGEIQFFEVADDY
                     DDEQLDAFTELSRRRGTAVVFPSFEYHRVLPLRSGVRHSLVSWVSGPPFR"
     gene            5834..6508
                     /locus_tag="PKY13_10710"
     CDS             5834..6508
                     /locus_tag="PKY13_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80450.1"
                     /translation="MPATSTVLDEENLVPAARFGAALHRERAQSGATMAAIARESDGR
                     FLPDQLVVVERGAATLSDADVALLARLYGLGERAWASASSLELVLDRAPMSDIGTGGP
                     TTSDRTREESLSWVAARFLALSVLLGIDVTSGPVGLDVLADAVELSLDATVDLVATTL
                     DNDAATVGSMIGEMESRVVVPEVGFLVAETTTGSLLLVGRGPSATDAVVVPACGALSD
                     LLEAVR"
     gene            complement(6561..>6892)
                     /locus_tag="PKY13_10715"
     CDS             complement(6561..>6892)
                     /locus_tag="PKY13_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80451.1"
                     /translation="AVLGADDDTYPMSGGNSPSFEETFGDRLGDGAEYKPAAPPAALD
                     AWADGLGCVSNATQQIGLATARIDTTCDDDGEVWRVVIPGAGHEWYPATYDLMVQFLT
                     TGPFRTT"
BASE COUNT         1015 a         2365 c         2338 g         1074 t
ORIGIN      
        1 agcacggccg agcgcaacga cgccaacgag tggattgccg agagctggga ggttcacgct
       61 gcgggtgtcg cgctcggcgt gctcattcag cacgaccttg aatgggcaga gccgaacccg
      121 accggatcgt gacggaggta ctggttgagc gatgggctga tgcgtgacgc cgagggtgcc
      181 catccggccg atgttgctga atggccggtg gaggccgccc gagcgacggt ggtgcgggaa
      241 gctcgcatga acagtctgcc ggactcgggt gatcaatcct tcggtcgaca ggagccgacg
      301 tctggcagcc agccgaactt cagctcgccg gagcgaagca tcttctcgat ccggcgagcc
      361 gactccacag ctcggtcatg tcgacggaac aaccggctac gtaccaccca gaggagcgaa
      421 gggtcgcgaa cgacgacggc ggaggagtcg gtgtgcaagg cagcggggtg gcgcttccgg
      481 gcgtgaccct cgtcgagcgg gtgacggtct gggaatgtca cccgatccga ggtcgaaccg
      541 gtgctgacac tctcgatcgc tcagacgtag tccgactgca cctcagcgat cgagagttca
      601 ccgccgccca gtcgacggtg aattcgtctc accggcagtt gtgcggcccc gccccgttca
      661 gagcggcgtc gtgtaggtct tcgtgatgcc gccgtcgatc aggagcgact ggcccgtcat
      721 gtacgagctc tcgtcgctgg cgaggaacag ggcgccgttg accatctcct cggcctcgcc
      781 gaagcgaccc atcgggatgt ggacgaggcg gcgctggcgc ttctcctcgt cggacaggaa
      841 cttggcgagg agcggggtga gcaccggacc ggggcagagt gagttggcac ggatgccctg
      901 gcgggcgtag atcgccgcga tctcctgggt catcgccagc acggcgccct tggacgaggt
      961 gtacgcgatc tgcggggtgg cggcacccat gtgggccacg aagctggcga cgttgacgat
     1021 cgagccgccg ccggagtcga gcatcgcctg gatcccgtac ttgttgcaga ggaacaggcc
     1081 cttcacgttg acgtcgaacg tgaactgcca cgtctcctcg ctcgtgttga gcggggtgtc
     1141 gtcgtcaccg ggggagacgc cagcgttgtt gtagaggatg tcgaggccgc cccagttgtc
     1201 gacggcgatc ttgaccatgg cctcgacctg gccggcgtcg cggacgtcga cggtcgcggg
     1261 gagcgcggtg ccgccggcgg cggtgatcgc ctcgaccgtc tcggcagcgc cgtcggcgtt
     1321 gttgtcggcg acgacgacct tggcgccctc ggcggcgaac cgctggcagg cgatccggcc
     1381 catgccggct ccgccgcccg tgatgatggc gatcttgtcg tcgaggcgac ccattgctgt
     1441 gctcctgagg gggtgacgtc ggggttctgt agtgcgagga gcgacgctcg cgtcgctcct
     1501 cgcactacaa aacccctggg ggcgatgaac ttccgttgcg gcgacacact atgggccggg
     1561 ggtacgatcc ggccatgtcg tcagcccccc ttcaccagcc cctcgccagc tcctacgacc
     1621 tcgtgatcgc cggcgagaag gtgcccgcag cggagggcac cacgttcacc gcttacgagc
     1681 cgtcgaccgg cgcagcgttg gccaccgtgg cccaggcggg cagcgtcgat gtcgaccggg
     1741 cgctgaacgc cgccgtcgcc gagttcgagg agggcacgtg gcgcaccacc aacgcgactg
     1801 cccgcggcaa ggtgctcctc aaggtcagcc agttgctccg cgagcgggcc gaggacttcg
     1861 cgatcgccga gtcccgcaac agcggccacc cgatcggcaa ctcccgctgg gaggccaatg
     1921 ccgccgccga cgtgttcgag tacttcgccg gcgccgccaa caagcactac ggccagacga
     1981 tccccgtgca ggacaacggc ctcgacgtcg tcctccgcga gccggtgggc gtgtgcggcc
     2041 tcatcgtccc gtggaacttc ccgctgctca tcacgacctg gaaggtcgcc cccgccctgg
     2101 cgtgtggcaa ccccgtggtc atcaagcccg ccaccctcac gccgatcacg gccctgatgc
     2161 tcgccgacgt gctgctcgac gccggcgtgc ccggcgggtg catctcggtc atccccggtc
     2221 ccggcgcatc ggtgggcgac aagttggccg gcgacccccg cgtcgccaag ctcagcttca
     2281 ccggtgacag ctcgacgggc gcgcagatca tgcgtcgctc gagcgacaac atcacccggc
     2341 tgtcgctcga gctcggcggc aagtcggcgt gcatcgtgtt cgaggacgcc gacatcgagg
     2401 cggcgatcgc cgcgactccc atgtcggtgt tcgacaacac cggccaggac tgctgcgccc
     2461 gcagccggtt catcgtccac cgcagcgtgt acgaccagtt cgtcgagggc ttcgtgaagg
     2521 tgaccgaggg cctcgtgacg ggcgaccccc tcgacgagtc gacccagctc ggcccgatga
     2581 tctcggaggg gcagcgtcag accagcctcg actatctggc gatcggcgcc gccgagggcg
     2641 cgaaccggct gtgcggcggc gaggagagcg gccccggttg gtacctcacg ccggccgtcc
     2701 tcgccgacgt cgacaactcc atgcgcgtcg cccaggagga gatcttcggt ccggtcgcct
     2761 cgatgattcc cttcgacacc gaggccgacg ccatccgcat cgccaacgac agcccctacg
     2821 gcttgtcggg ctcgttgtgg accaacgacc tcgggcgctc catccgggtg acccgcgcgg
     2881 tgcggaccgg tgtcatttcg gtcaactcga cgcgtagcgt acgcaccgag gcgccgttcg
     2941 gcggcttcaa gcgttctggc atgggccgcg agctcggcat ggcggcgatg gaccactaca
     3001 ccgaggtcaa gaacgtcttc tactcccaag actgagggcc acatgagccg atcgatgatg
     3061 acactgccga cagcggccga tccggcgtcg gtggtcgaca ccgacgcgta tccgttcggc
     3121 gacagtgact gctacccgtg gatcctggcc gcccgcgccg atctcgccgc cgacggcgtg
     3181 gcgatccttc cgggcttcgt ccgtcctgag gcggttgcga cgatgtgcgt cgaagccgac
     3241 cgtctggccc cgctcggcca ccattccgtc gtccagggca ccccgtatct cgggttgccc
     3301 gacgagtcgt ttcccgaggg ccacccgcgt cgggccctcg tcgacaattc gctgacggcc
     3361 gtcgcctacg acctcatcgg agccgactcg ttgctccgcg tgctgtacga gtggccacgg
     3421 ttcaccgaga tgatccgccg catgctcgac cgccctgcgt tgtaccacta cgccgacccg
     3481 ttcggtgcgc tcaacatcgc cgccatgagc gccggcgacg aactgggatg gcacttcgac
     3541 caaaccgact tcgtggtgtc gatcgccctg cagtcgagct ccggcggcgg tgacttcgag
     3601 agcgtggccc gcctgcgcag cgccgaggac gatgcgtacg acgaggtgtc ggccgtgctc
     3661 gccggagatc gttcgcgggt cagcagcgtg cccatgactc ccggcacgct catgttgttc
     3721 gagggtcgca attcgttgca ccgggtcacc aagatcgagg gtgacgttcc ccgccacgtg
     3781 gcgttgctcg cgtacgacac cgttcccggt accgattcga gtgaactgtt gaagctcgtg
     3841 cggtacggca ggtcgcccga gaccgtcgcc gggcccggtg ttcgatccga cggaggacaa
     3901 cagcaatgac gaggaacgga ggtcggccgt gagctggacc ggcatgctca tcggcgaggg
     3961 gttcgagggc agcggcacgt cggcggcaca catcaacgcc gtgctcggcg accgcacgag
     4021 ccccgtcggc caggcctggg tccaggcgct cgcgacgccg agcgccgggc acgcgccctt
     4081 catggtcgtg gctcgccccg ggctccccgt gaagccgttc accctgttcg tctccaaggc
     4141 gaccacctcg aaccgcgacc acgccgacgt gacgtggggc gcggcgcagg ccggggtcgc
     4201 cgcgggtgtc gtcgaggcgg tctcggccgg catcattccg accgacgtcg cggaggaggc
     4261 cctcctcatc tgcgccgtgt gggtcgaccc gggcgcgacg gtcgcccatt ccgacgtcgt
     4321 gttcctcaac aaccgggtgg ccacctacgc cgccctcgag aatgccggcc accaccgccc
     4381 gtcggtcgcc gacgtgctct ccgacgacca gggcatctgg aatcccttct accggccgtg
     4441 acgaccaacg tcgtgacgac ccacgccgtg acgcgcccgg tctgtcccgc gagggcgtgc
     4501 gatctgcacc catccgcggg acagaacggg cggcgnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnntctgt cccgcgaggg cgtgcgatct gcacccatcc gcgggacaga
     4681 acgggcggcg cggcagaatt gctcggctcg agcgtcgtgg gacagagtgt cgggtccccg
     4741 agagcaccgg atgactctgg agagaatcga acgatgactg gtccccgcaa gaagatctgc
     4801 gccctcgtgc tggcggcatc cgtgtccgtc ggccttgccg gctgctcccg tcccgatgcc
     4861 aacgcaccgg tgcgcatcac gggcgacaag gacgcggcga tccccgccac cgatccgacg
     4921 accaccacct cgacgaccga ggcgcccccg acgacgttgt tctacagccc cgacggcccc
     4981 ggctcgaaga tcgctgccga gacgaccacg acgaccgagg ccgagcccac caccaccgag
     5041 gccgagtagt cccggccgtc ggcccgacgc tctcccgcac caccttggaa cctctcaccg
     5101 gtcggcccct gctcgggacc ccggttcccc cgccgtacca ggcgttcgag ctgcacccga
     5161 acgccttcac ccctcgccag tgcgaccgga tcgtcgcgct cggtgagcag ctgcgggccg
     5221 agtcggccgg gctcgagggc accgacggcg acgaggtgaa cgacgagtcc atccgccggt
     5281 cacgaaccgc ctggatcgcc cccgacgacg acagctggtg ggtgtacgac aagctcgcca
     5341 agatcgccga acgggcgaac cgccggtacg gcttcgacct gacgggattc ggcgaggacc
     5401 tccagttcac gttctacgac gagcccggtg cgttctactc gtggcaccag gacgggctcg
     5461 acggtggggt cgccacccgc aagttgtcga tcgtgatcca gctgaccgat cccgaggact
     5521 acgagggcgg cgagatccag ttcttcgagg tggccgacga ctacgacgac gagcagctcg
     5581 atgccttcac cgagctgagc cgccggcggg ggaccgccgt cgtcttcccc tccttcgaat
     5641 accaccgggt gctgccgctg cgttcgggtg ttcggcactc cctcgtgtcg tgggtgagcg
     5701 ggccgccgtt ccggtgaccc ccgttcgtca gcaccgatcc acgggcggtg cggacgaacc
     5761 ccggccatag gtcgggtggc cgtttggcgc cgccgatcct caaccggacg gcgggacgcg
     5821 ccgatgagac tgcgtgcccg caacgtcaac cgtcctcgac gaggagaacc tcgtccccgc
     5881 cgcccggttc ggtgctgcgc tgcaccgcga gcgcgcacag tccggcgcga cgatggcggc
     5941 gatcgcccgt gaatccgacg gacggttcct tcccgaccag ctggtcgtgg tcgagcgagg
     6001 tgcggcgacg ttgtccgacg ccgacgtggc gctgctcgcc cgcctctacg ggctcggcga
     6061 gcgtgcgtgg gcctcggcct cgtcgctcga actggtgctc gaccgggccc ccatgagcga
     6121 catcgggacg ggcggtccga cgacgtcgga ccggacccgc gaggagtcgc tcagctgggt
     6181 ggcggcccga ttcctcgccc tgtcggtgct cctcgggatc gacgtgacgt cggggcccgt
     6241 cggcttggat gtgctcgccg acgcggtgga gctgtcgctc gacgcgaccg tcgacctggt
     6301 ggccacgacg ctcgacaacg acgccgccac cgtcgggtcg atgatcggcg agatggagtc
     6361 gcgcgtggtg gtgcccgagg tcgggttcct cgtcgccgag acgacgaccg ggtcgttgct
     6421 cctcgtcgga cgtggcccgt cggcgaccga cgcggtcgtg gtgccggcgt gtggggcgct
     6481 gtccgatctg ctcgaagccg tccgataggc cgacgcccgt tggtgccggc cggacccgtc
     6541 aggtcggtcg gagcggccgg tcaggtcgtg cggaacggcc ccgtcgtgag gaactgcacc
     6601 atcaggtcgt aggtcgcggg gtaccactcg tggccggcgc cggggatgac gacgcgccag
     6661 acctcgccgt cgtcgtcgca cgtcgtgtcg atccgggcgg tggcgagccc gatctgttgg
     6721 gtcgcgttgc tgacacagcc gagtccgtcg gcccaggcgt cgagcgctgc cgggggtgcc
     6781 gcgggtttgt actccgcgcc gtcgccgagg cggtcgccga acgtctcctc gaacgagggg
     6841 gagttgccgc ccgacatcgg gtacgtgtcg tcgtcggcgc cgaggaccgc ga
//
LOCUS       DAOSOA010000166         6869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11115_length_6869_cov_4.501027, whole genome shotgun sequence.
ACCESSION   DAOSOA010000166 DAOSOA010000000
VERSION     DAOSOA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11115_length_6869_cov_4.501027"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..906
                     /locus_tag="PKY13_10720"
     CDS             184..906
                     /locus_tag="PKY13_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80452.1"
                     /translation="MPAHRSSNLRRYLTLAMAVGALLAACTPQPSGPTEPTSTTTASS
                     TTTVPGPAASISLTIPLPGSELDSSFAAAVDVESPPPGGWIRYSLAGSVVFQEAAGVT
                     TTVLDGGTTTGPAELLVELLDDHNNVVASDSTGVLLLGTPVPSLDDLHAAFERAELSP
                     DQLLLRGAAAIMAMDQAEDHIGELTPIVLSLAAAAYSSALPEAQRAFAALSGGTELGG
                     TSISGVLRRGRPTTPTAHSCRD"
     gene            1083..3488
                     /locus_tag="PKY13_10725"
     CDS             1083..3488
                     /locus_tag="PKY13_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80453.1"
                     /translation="MLTGDDTPDVVERAAIKLWKAQAVWSEMGFVLPQEHRGGTPVTI
                     VVTNNNLAGYSSPFAVTRPGTNLGHMIRVSGPKGHLASIVVHEFFHTHEWSWINWNAD
                     IGLGDDGRGVTFYESAANWGREQYAARYEPSLDGDFTQAVDEWLSQPARAFLTRAAKA
                     TPGAQAYGWFTALEWLDEHGGADEFDTSGLIRDTLRSYTDHSFGAEPIDLISDQLDRG
                     AYATDDTYRTSLAWPRMWTALYLLRDGATQADVFAEPEWRWSMFWNHPLERLSDELDR
                     SPATKRDEFGKARPARITDSPVALTLNDGTWSTEVEIDRGGAAFVDVTTPAEKRGTVE
                     VELVPDDDGDAPKVGGLAISYNRTGHPEVCVRDDRPELHVSDGTHNDDGSIVFEVPFD
                     PDCPTMTVGLVHAEPASEDPLAATISLRLTSVQGAPYEDSRDHTCVAAGIALQNAKAW
                     CWGANSYGQVDPGTQTDVVLQPELVDGLTLPRGPAPGGGHTCAFDYSEVIDLGPDGVS
                     VTYNPRFSCWGRNDHGQLGSGTTTDQFRAVQVALPAAPVRVTTGMTHTCAIAKVGAEP
                     DSLWCWGSNRYGELGDGTTVQRTSPVRVPGTDGVTRVSAGDGYTCAVIDGTTSCWGRN
                     DHGELGLGTIDSLPHPSPQPVALAGLTGVAAGRGTTCAIGIDARAYCWGRNHRGQVGN
                     GTLTDTATPDRVELPAGVTTRQITVGGGHACATTAVGDAPTVVYCWGRNDSSQTGLDL
                     SESEPPHPVPSVDGAAQVVAYDRHTCVVLTTLRCFGDNSRGQLGNGTTVTTKEPQTVA
                     IPG"
     gene            complement(3600..>3828)
                     /locus_tag="PKY13_10730"
     CDS             complement(3600..>3828)
                     /locus_tag="PKY13_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80454.1"
                     /translation="AEAGPNAAALTSVTVNVNDVQYLSATRARVVYSLSVPDQPPVVG
                     REGQARVASGAWKVTRATVCADLDAIGSACS"
     assembly_gap    3829..3928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4056..5732)
                     /gene="ilvD"
                     /locus_tag="PKY13_10735"
     CDS             complement(4056..5732)
                     /gene="ilvD"
                     /locus_tag="PKY13_10735"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOT80455.1"
                     /translation="MTTMKPRSTDVTEGFQKAPARAMLRAIGMSDGDWDRTQVGVASS
                     WNEVTPCNMPLDRLAKQAKLGVHSAGGFPIEFNTIAVSDGISMGHEGMRASLVSREVI
                     ADSVETVMHAERMDALVSFAGCDKSLPGMMMASARLNLPSVFLYGGSKLPGQLNGRAL
                     DITSVFEAVGAVAAGNMEQSELDQIERNACPTIGSCAGMFTANTMASVGEALGLSLPG
                     SASAPAVDRRRDDYAFASGVAVINLVRENIRPRDIITRNSLMNAIAVVNALGGSTNAV
                     LHLLAIAYEAEVELELQDFNRVAAKVPHLADTKPHGKYHMLDIDRVGGVPVVMQMLAD
                     EGLLHLDEITVTGKTVGENLELIKAPEPDGKVIHAFSDPIHQLGGLAVLEGSLAPNGA
                     VLKVAGIDFDFFEGPARVFDGEDKAMEAVLGEQINPGDIVVIRYEGPKGGPGMREMLA
                     ITGAIKGVGRGGDTALITDGRFSGGTHGFCIGHVAPEAVDGGPIALVAEGDLIRIDVK
                     NHTIDLLVDDEVLAERRLDLTYPEPRYTKGVLAKYAKLALGAEFGAVTSA"
     gene            5809..>6869
                     /locus_tag="PKY13_10740"
     CDS             5809..>6869
                     /locus_tag="PKY13_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT80456.1"
                     /translation="MSSSRRTIDRQIAAVAIPAALALATDPLYDLCDTAILGHIGTAQ
                     LAGAALATRILAFGYAAFVFLMFGTTAAVARHHGAGREREAAEQGVGAMWLAAGCGLL
                     AAGVFAVVGRSLIGWFGGTSDVARYAWTYLWVSLGGLPAFTMVMAGVGFLRGGQDTRR
                     PLAVSAVTVTLNLVLEVLFVYGAGLGVGASALGTVIAKWVGAAAYLTLLGRSVRRHGA
                     SWRPQPAAIRGQLVVGRDLVVRTVVLLGVFSAAQAGAARVGVDELAAYAIAFQLWMIG
                     AYAVDGIEAAGQSLIAHRIGAGRTDEVRAVVGRLVRWALTLGCSVGAILAATSALSPH
                     LFTNDTNVVAAASASLLWVA"
BASE COUNT         1063 a         2434 c         2235 g         1037 t
ORIGIN      
        1 tccgcgaccc cctcaccctc cacaacagca ccgccaacac caccaccgaa ctcgcccccg
       61 ccggcacctg caccaacacc tacgacgtcc ccgtcttcgc cgccccacca ggagcattcc
      121 caccaccatg atgtgtagtt cttcctaagt acacgaaagt tggtggaagg taccgtcgct
      181 ccgatgccag cacaccgcag ttccaacctt cgcaggtacc tcacgctggc catggccgtc
      241 ggcgcactgc tggccgcatg cacaccccaa ccatccggcc cgaccgagcc gacctccaca
      301 acaacggcct cgtccaccac tacggtgccc ggtcctgcgg cctcgatctc gctcacgatc
      361 cccttaccgg ggtcggagtt ggattcgtcg ttcgccgctg cggtcgacgt cgagtcaccg
      421 ccaccggggg gatggattcg ctactcgctc gccggctccg tcgtgtttca ggaggcagcg
      481 ggagtaacga ccaccgtgct ggacggcggc acaacgaccg gccccgccga gctcttggtc
      541 gaactgctcg acgatcacaa caacgtcgtc gcaagcgact ccaccggggt gcttctcctc
      601 ggcacgccgg tgccgtcact cgacgacttg cacgccgcgt tcgagcgggc ggaactctcc
      661 cccgaccaac tgctgctccg cggcgccgcc gccatcatgg ccatggacca agccgaggac
      721 cacatcggcg agctcacgcc aatcgtgctc tcgctggccg ccgccgcgta cagctcggcg
      781 ctgcccgaag ctcaacgggc attcgccgcg ctgagtggcg ggaccgaact cggaggaacc
      841 tcgatcagcg gcgtcctgcg gcgcgggcgg ccgacgaccc cgactgccca ctcctgcagg
      901 gactgacgtg ggggttcggg ctcatctgga acgacgactt ttgttcgtac ctggttcaac
      961 cgggcgacct cgccgaccgc ctgcccgaca gtgtcgatcc cgccgatctc gtttcgctgc
     1021 ggttcctcgt cgatccccga ctcgacgaca ccgacctcga cagcgacgga atcgacgacg
     1081 cagtgctcac cggtgacgac accccagatg tcgtggagcg ggccgcgatc aagctctgga
     1141 aggcccaggc ggtgtggtcc gagatggggt tcgtcctacc gcaagagcac cgcggtggca
     1201 cgccggtcac catcgtcgtc accaacaaca accttgcggg gtactcgtcg ccgttcgcgg
     1261 tgacccggcc cggtacgaac ctcggccaca tgatccgggt gtcagggccg aagggccacc
     1321 tggcgagcat tgtggtccac gagttcttcc acacccacga gtggtcgtgg atcaactgga
     1381 acgccgatat cgggctgggc gacgacgggc ggggcgtgac gttctacgag tcggctgcca
     1441 actggggccg cgagcagtac gcggcccgct acgagccgtc gctcgatggg gacttcacgc
     1501 aagctgtcga cgagtggctc agccagccgg cccgagcgtt cctcacccgc gccgccaaag
     1561 cgacaccggg cgcccaggcc tacggatggt tcaccgccct cgaatggctc gacgagcacg
     1621 gtggcgccga cgagttcgac acctccgggc tgatccgcga cacgctccgg agctacaccg
     1681 accactcctt cggtgccgaa ccgatcgacc tgatctccga ccaactcgac cgcggcgcgt
     1741 acgccaccga cgacacgtac cgcacctccc tcgcctggcc ccgcatgtgg acggcgctct
     1801 acctcctccg ggacggtgcc acccaggccg acgtgttcgc cgagcccgag tggcgttggt
     1861 cgatgttctg gaaccatccg ctggaacgat tgagcgacga gctcgatcga agcccagcca
     1921 cgaagcgcga cgagttcgga aaggcccgtc ccgcccggat caccgactcg ccggtcgcac
     1981 tcacactcaa cgacggcacc tggagcaccg aggtcgaaat cgaccggggc ggcgccgcct
     2041 tcgtcgacgt caccacgccc gccgagaagc gcggcaccgt cgaggtcgaa ctcgttcccg
     2101 acgacgacgg cgacgcaccc aaggtcggcg gcctggccat ctcctacaac cgcaccggcc
     2161 accccgaggt gtgtgtccgc gacgaccggc ccgagctgca cgtgagcgac gggacccaca
     2221 acgacgacgg cagcatcgtc ttcgaggtgc cgttcgaccc cgactgcccg acgatgacgg
     2281 tcggcctggt ccacgcggaa ccggcgagtg aggatcccct tgccgcgacc atcagcctcc
     2341 gactcacgtc cgttcagggc gcgccctacg aggactcccg agaccacacg tgcgtcgcgg
     2401 cgggaatcgc tcttcagaac gccaaggcgt ggtgctgggg agcgaactcc tacggccagg
     2461 tcgatccggg gacgcagacc gatgttgtgc tccaacctga actcgtcgac ggtctcacgt
     2521 tgccccgtgg cccggcgccc ggaggtgggc acacgtgtgc gttcgactac agcgaagtca
     2581 tcgacctcgg tcccgatgga gtctcggtca cgtacaaccc ccggttcagc tgctggggtc
     2641 gcaacgacca cggacagctc ggtagtggaa ccaccaccga ccagttcagg gcggtgcagg
     2701 tcgcattgcc cgcagccccg gtgcgggtca cgaccgggat gacccacacg tgtgcgatcg
     2761 ccaaggtcgg agccgaaccc gactcgctgt ggtgttgggg ttcgaaccgg tacggcgagc
     2821 tcggtgacgg gacgacggtg caacgcacct ccccggttcg cgtccccggc accgacggtg
     2881 tgaccagggt gtcggccggc gacggctaca cctgcgctgt catcgacggc acgacttcct
     2941 gttggggccg aaacgaccac ggcgaactcg gcctcgggac gatcgactcg cttccgcacc
     3001 cttcgcccca gccggtggcc ctcgccgggt tgaccggcgt cgccgccgga cggggcacca
     3061 cgtgcgcgat cggcatcgac gctcgcgcct actgctgggg aaggaaccac cggggacagg
     3121 tcggcaacgg cacgctcacc gacacagcga cgcctgatcg ggtcgagctg cccgctggag
     3181 taacgacacg ccagatcacc gtcggcggcg gccatgcgtg cgcgaccacc gcagtcggcg
     3241 acgccccgac cgtcgtctac tgctggggtc gaaacgactc gagccaaacg gggctcgact
     3301 tgtccgagag cgaacctcca cacccggtgc cgtcggtcga cggggcggcc caggtcgtcg
     3361 cctacgaccg gcacacctgt gtcgtgctga cgaccctgcg atgcttcggg gacaacagcc
     3421 gaggccagct cggcaacggc accacggtca ccaccaagga accacagacc gtcgcgatcc
     3481 ctggctgacg gcggatcgcc caaggccacc agtttcgcgg gtagttatcg ccaccccctg
     3541 ggaacaactt cccgcgaaac cggggcgttg cgctcgacga tggcgggtga gggcggccct
     3601 caactgcagg cgctgccgat ggcgtcgagg tcggcgcaca ccgtcgctcg ggtcaccttc
     3661 cacgctcccg aggcgacgcg agcctgtccc tcgcggccga cgaccggcgg ctggtccggc
     3721 accgacaacg agtagacgac gcgagcccgc gtcgccgaca ggtactgcac gtcgttgacg
     3781 ttcacggtga ccgacgtgag tgccgcggcg ttggggccgg cctcggccnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gtcctcgagg tcactgaccc ggatgtgggc
     3961 gagcacggcg aacaccaggg ccccgagggc gaggacgagc gcggcggccg cccacgcccc
     4021 cgctgacgaa ccgcgctcgc gctcgacctc gggtatcaag ccgaggtcac cgccccgaac
     4081 tcggcgccga gtgcgagctt cgcgtacttg gcgagcacac ccttggtgta gcggggctcc
     4141 gggtacgtga ggtcgagccg gcgttccgcc agcacctcat cgtcgacgag gaggtcgatc
     4201 gtgtggttct tgacgtcgat gcggatcagg tcgccttcgg cgaccagcgc gatcggaccg
     4261 ccgtcgaccg cctcgggggc gacgtggccg atgcagaatc cgtgggtgcc acccgagaac
     4321 cggccgtcgg tgatgagcgc cgtgtcgcca ccgcggccga cacccttgat ggcgccggtg
     4381 atggcgagca tctcgcgcat gcccgggcca cccttcggac cctcgtagcg gatgaccacg
     4441 atgtcgccgg gattgatctg ctcgccgagc acggcctcca tcgccttgtc ctcgccgtcg
     4501 aacacacggg ccggaccctc gaagaagtcg aagtcgatgc ccgccacctt gagcaccgca
     4561 ccgttggggg cgagcgaccc ctcgaggacg gcgaggccac cgagctggtg gatcgggtcg
     4621 ctgaacgcgt ggatgacctt gccgtcgggc tcgggcgcct tgatgagctc gaggttctcg
     4681 ccgacggtct tgccggtgac ggtgatctcg tcgaggtgca gcaggccctc gtccgccagc
     4741 atctgcatca cgaccggcac accaccgacc cggtcgatgt cgagcatgtg gtacttgccg
     4801 tgaggcttgg tgtcggcgag gtggggcact ttcgcggcga cccggttgaa gtcctgcaac
     4861 tccagctcga cctcggcctc gtaggcgatg gcgaggaggt ggaggaccgc gttggtggag
     4921 ccgcccagcg cgttgacgac ggcgatggcg ttcatcagcg agttgcgggt gatgatgtcg
     4981 cggggccgga tgttctcgcg aacgagattg atgacggcga cgcccgaggc gaacgcgtag
     5041 tcgtcgcggc ggcgatcgac cgctggagcc gacgccgaac ccggcagcga caacccgagg
     5101 gcctcgccca ccgacgccat ggtgttggcg gtgaacatgc cggcgcagct gccgatcgtc
     5161 gggcaggcgt tgcgttcgat ctggtcgagc tcgctctgct ccatgttgcc ggcggcgacg
     5221 gcgccgaccg cctcgaacac gctggtgatg tcgagcgcac gaccgttgag ctggccgggg
     5281 agcttcgacc cgccgtacag gaagaccgac gggaggttga ggcgggccga cgccatcatc
     5341 atgccgggca gcgacttgtc gcagccggcg aagctcacga gtgcgtccat acgctcggcg
     5401 tgcatcaccg tctcgacgga atcggcgatc acctcacgac tgacgagcga cgcccgcatt
     5461 ccctcgtggc ccatcgagat gccgtcggac acggcgatgg tgttgaactc gatcgggaag
     5521 ccgccagcgg agtgcacccc gagcttcgcc tgcttggcga gccgatcgag cggcatgttg
     5581 cacggcgtca cctcgttcca cgacgacgcc acccccacct gcgtacggtc ccagtcgccg
     5641 tcgctcatgc cgatggcgcg cagcatcgcg cgggcaggcg ccttctggaa tccttcggtg
     5701 acgtcggtgg accggggctt catcgtggtc atggccgcag gttatgcgag cggggccgcg
     5761 ccacgggatt cgaatacgga ccgaccccga gggtcctacg ctccggcgat gtcgtcgagc
     5821 cgccgcacca tcgaccgaca gatcgcggca gtggcgatcc ccgccgcgct cgcgctggca
     5881 accgatccgc tgtacgacct ctgcgacacc gccatcctcg gacacatcgg cacggcacag
     5941 ctcgccgggg cggcgctcgc cactcgcatc cttgccttcg gttacgccgc cttcgtgttc
     6001 ctcatgttcg gcacgaccgc ggcggtcgcc cgccaccacg gggccggtcg cgaacgtgag
     6061 gccgccgagc agggggtcgg cgccatgtgg ctcgctgctg gttgcggcct gctggcggcc
     6121 ggggtcttcg ccgtggtcgg tcgctccctg atcggttggt tcggcggcac cagcgacgtc
     6181 gcccgatacg cctggacgta cctctgggtc agcctcggcg gactcccggc gttcaccatg
     6241 gtgatggcgg gcgtcgggtt cctccgcggt gggcaggaca cccgtcggcc tctcgcagtg
     6301 tccgctgtca ccgtcacgct caacctcgtc ctcgaagtgc tgttcgtcta cggcgccggg
     6361 ctcggggtcg gagcatccgc gctgggcacg gtgatcgcga agtgggtcgg cgcggcggcg
     6421 tacctgacac tgctcggccg gagtgtgcga cgccacggcg catcgtggcg cccccaaccg
     6481 gcggccatcc gtggtcagct cgtcgtcggt cgcgacctcg tcgtccgcac cgtcgtgctc
     6541 ctcggggtgt tcagcgcggc tcaggccggc gcggcccggg tcggcgtcga cgagctcgcc
     6601 gcgtacgcga tcgcgttcca gctgtggatg atcggggcgt acgccgtcga cgggatcgag
     6661 gccgccggcc agtcgttgat cgcccatcgc atcggcgccg gacgcaccga cgaggtccgc
     6721 gccgtcgtcg gccggctcgt ccggtgggcg ctcacgctcg ggtgcagcgt gggggcgatc
     6781 ctcgccgcga cctcggcact cagcccccac ctgttcacca acgacacgaa cgtggtcgcc
     6841 gccgcctcgg cgtcgctcct gtgggtcgc
//
LOCUS       DAOSOA010000167         6825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11201_length_6825_cov_6.086854, whole genome shotgun sequence.
ACCESSION   DAOSOA010000167 DAOSOA010000000
VERSION     DAOSOA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11201_length_6825_cov_6.086854"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..647)
                     /locus_tag="PKY13_10745"
     CDS             complement(36..647)
                     /locus_tag="PKY13_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80457.1"
                     /translation="MDSTDVLGIVASAVFLARLTPQPVRLARSGVADGVSPLAALNAV
                     IAALAWLVYGLVADDPVVWIVSLVAVVPGVWAVLLLRRRTALADLLAAGAWAGIVVAS
                     AAAGFLAAALAMGVVVSQGPQVWQAVREHDLSGLSPATWWLSILDAATWGAYGLAVGD
                     PALKGYAVVLTLSAVVVLARISWTRRVDQPVNADMISAPNRAS"
     gene            complement(655..1773)
                     /gene="ald"
                     /locus_tag="PKY13_10750"
     CDS             complement(655..1773)
                     /gene="ald"
                     /locus_tag="PKY13_10750"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOT80458.1"
                     /translation="MRVGVPREIKNAERRVGLTPASVRELVAHGHEVVVERHAGVGIG
                     ASDADYAAAGATLHNTAAEVFAAADMIVKVKEPQASERAMLRPGQVLFTYLHLAPDPD
                     QTADLVASGAVCIAYETVTDSQGGLPLLAPMSQVAGRMSIQAGAHCLESAQGGAGVLL
                     GGVPGVAPGKVVVIGGGVVGENAIQMAVGLDADVTVLDRDVTVLDRLSRRFGSTLRTQ
                     YSTRTALDDLVVNADLVIGAVLVKGSRAPHLVTADMVRAMRAGSVLVDVAIDQGGCFE
                     TSHATTHDDPTYVVDDVVHYCVANMPGAVPHTSTHALNNVTLPFVLALADQGATAALL
                     ANPHLLGGLNVCRGLVTESAVAEALGYEYVPPADALAR"
     gene            1853..2599
                     /locus_tag="PKY13_10755"
     CDS             1853..2599
                     /locus_tag="PKY13_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT80459.1"
                     /translation="MAKSAGKVEQNRQHRMRRFADASLHIVATEGLAALTMARLAEEL
                     DTVPSAVYRYFASKGALITAVQCDAIERLTASYGLIRTLSEDHFVELGLDEADTVVAR
                     LVLFGRWFCATADTHLEELRLLQMIMSERTHDLDPQGGFEVLPIAMVLLGQAAECLDA
                     AEGIGLATPGESLERVIVWAAALGGVLETDSLARYVPELLGDGRLARRTNLDLLRGWG
                     VPDDRLIAATHVVDDLATRGSLAPAVERAS"
     gene            2596..3972
                     /locus_tag="PKY13_10760"
     CDS             2596..3972
                     /locus_tag="PKY13_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT80460.1"
                     /translation="MTLARLVGDELTVPCIDGTDRVYLGLDAGASTAAFESVAARVQE
                     FLPWYSSVHRGAGRKSQVATQAYEDAREAMLEFAGRGDSDDVAIFCRNTTEAINILAY
                     RLDVEPGDTIVTTVAEHHANLLPWQRLAHERGVTIRYVECDERGEFAAEDVAEAIETA
                     DRPRLLAITGATNVTGWMPPLAGIIDHAHRHGAEVLVDAAQLAPHRPLATSGAAAADY
                     LAWSGHKMYAPFGTGVLIGPRRTFETGTPLLVGGGAVNLVDLDEVLWTDPPERDEAGS
                     PNVLGAVALHAAVDEFARIGWDAIVAHEEALATRLRVGLRDLAGVRVLGEVPDRDDRD
                     YLAVGSFVIGTAGSGSAGPLPHPLVAARLSAEHAIGVRHGCFCAHPYLVRLLGLSNEE
                     TARYRHDIVVGDRRNIPGAVRASVSISTTEADVDRFLDALDRVISGPEAPVRYRQDPT
                     TGDYWPVP"
     gene            complement(4028..4450)
                     /locus_tag="PKY13_10765"
     CDS             complement(4028..4450)
                     /locus_tag="PKY13_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOT80461.1"
                     /translation="MRSPVSVHRRVRVAVAALLTVAVPAALSACTDDSGPPVKLSAEG
                     TKGKTVAARFQCQNCHSVDGDELTGPTWKGLLGSKVTLKDGSVTTVDADYLVRAVREP
                     NAQRREDATGQMPTFDEDRLSDEELAQVVAYITDLSAK"
     gene            complement(4461..5306)
                     /locus_tag="PKY13_10770"
     CDS             complement(4461..5306)
                     /locus_tag="PKY13_10770"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOT80462.1"
                     /translation="MRWWCSNDGSNWSWTWKPYIGAWAIVAAVAVWLWRSGAFRSEVP
                     ARRKVSLALGLVLILGGTDWPLSTLGAGYVVSAQMVRQVLIVMIACPLVLYGAPESLG
                     RWLEATPRRRAVLRIATHPLLALVVSVSLLFAVSAPILVDPLVTSQLGSFALDLAWIS
                     AGFLIWMPVQPPSPMVPRLRGPVAIVYLIGVSVAPLPVAFFMTWSPTPIYSVYELAPR
                     VWDTFDAQSDQELAAAIFQVAGGLVIWAQIAVRFVRMAGGGGGSAAGPKFRGTLVGAT
                     PATDG"
     gene            complement(5350..6018)
                     /locus_tag="PKY13_10775"
     CDS             complement(5350..6018)
                     /locus_tag="PKY13_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOT80463.1"
                     /translation="MAEHDRRSFLRASGLAALGVAAGAGLVTACSDDDSAALDDDNLG
                     FAGTVLGYPVAKPTIAFTDTSGAPYDVAAETVGKLTLMLFGYTSCPDVCPVHLSILAD
                     TLADLTGPASKTNVIFVGVDTQRDTPEVMRTYLDKRNPDFVGLSADPAQLDEALASMQ
                     LPSIIIGEPRDDGSYVVEHPSQILAFTPDNECHIVYPFGIRKAAWLKDLPRLIDYEWP
                     VTTR"
     gene            complement(6011..6484)
                     /locus_tag="PKY13_10780"
     CDS             complement(6011..6484)
                     /locus_tag="PKY13_10780"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOT80464.1"
                     /translation="MPRRSSALRFVVPLVGSLGVAALVGCGPGTSRIEVADAVVPVPA
                     TADTAAIYLRITNDGDADDRLLRVSTSRAELTTLHETTVDPDGRATMAGVGSVKIPAG
                     ETVSLSPGGLHLMMLQPDRLAAGDTVDLTLTFEQAGTVTVPATVTDDIDDVVGRG"
     gene            complement(6590..>6825)
                     /locus_tag="PKY13_10785"
     CDS             complement(6590..>6825)
                     /locus_tag="PKY13_10785"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80465.1"
                     /translation="DGAEMRSRFWMGGSHIALRGDRPGAALADRAARPVIARLLGASA
                     QSAADLLIHCAQEMSHLAAFLPELHADVTGSVL"
BASE COUNT         1071 a         2421 c         2423 g          910 t
ORIGIN      
        1 gagtggttgt gggacgaccc ggccgaacgc gccgatcagc tggcgcggtt cggtgcggag
       61 atcatgtcgg cgttcaccgg ctgatcgact cgccgcgtcc acgagattcg ggcgagcacc
      121 acgaccgccg acaacgtgag gacgaccgcg tagcccttga gtgccgggtc accgacggcc
      181 aggccgtacg caccccaggt ggcggcgtcg aggatcgaca gccaccacgt cgccggcgac
      241 aaccctgaga ggtcgtgctc gcgaaccgct tgccacacct gcgggccctg gctcacgacg
      301 acgcccatcg cgagcgcagc cgcgaggaat ccggcggcgg cggacgcgac gacgatgccg
      361 gcccacgccc cggcggcgag caggtcggcg agcgccgtgc ggcgccgcaa gagcagcacc
      421 gcccacacgc cgggcaccac ggccaccagg gagacgatcc agacgacggg atcgtcggcc
      481 acgaggccgt agacgagcca cgccaacgcg gcgatgacgg cattgagcgc cgccagcggc
      541 gacaccccgt cggcgacgcc ggaacgggcc aaccggaccg gctgtggcgt caggcgggcg
      601 aggaagacgg cggacgccac gatgcccagg acgtcggtgg aatccatcgc cacctcagcg
      661 tgcgagggcg tcggccggcg gcacgtactc gtacccgagc gcttcggcca ccgccgactc
      721 cgtgacaagt ccacgacaga cgttgagacc gccgaggagg tgcggattcg ccagcagggc
      781 ggccgtcgcg ccctggtcgg cgagcgccag cacgaacggc aacgtgacgt tgttgagcgc
      841 gtgggtcgac gtgtggggaa cggcaccggg catgttggcg acgcagtagt gaacgacgtc
      901 gtcgaccacg taggtggggt cgtcgtgcgt cgtggcgtgc gacgtctcga aacagccgcc
      961 ctggtcgatg gcgacgtcga cgagtaccga accggcccgc atcgctcgca ccatgtcggc
     1021 ggtgacgagg tgcggggccc gcgacccctt cacgaggacc gctccgatca cgaggtcggc
     1081 gttcacgacg aggtcgtcga gtgccgtccg ggtcgagtac tgcgtgcgga gcgtcgaacc
     1141 gaaccgccgt gacaggcggt cgagcacggt cacgtcacgg tcgagcacgg tcacatcggc
     1201 gtcgaggccg acggccatct ggatcgcgtt ctcgccgacg accccgccgc cgatgaccac
     1261 gaccttgccg ggagcgaccc cggggacgcc gccgagcaac acaccggcgc cgccctgcgc
     1321 actctcgagg cagtgggccc cggcctggat ggacatgcgg ccggcgacct gcgacatcgg
     1381 ggcgagcagg ggcagcccgc cctgggaatc ggtcaccgtc tcgtaggcga tgcacaccgc
     1441 gccgctcgcg acgaggtcgg cggtctggtc ggggtcgggg gcgagatgca ggtacgtgaa
     1501 caacacctga ccggggcgaa gcatggcccg ctccgatgct tggggttcct tcaccttgac
     1561 gatcatgtcg gcggcggcga acacctcggc cgcggtgttg tgcagggtcg ctcccgccgc
     1621 ggcgtagtcg gcgtcggacg cgccgatgcc gaccccggcg tgtcgctcga ccacgacctc
     1681 gtggccgtgg gccaccagct cccggacgct cgcgggcgtg agcccgacgc gtcgctctgc
     1741 gttcttgatc tcgcggggca ctccgacacg catgggccca ccctacgccc gcatggtcac
     1801 gacggggccg tccgatggtg agagaatgtc ggcgatcaga aggggccgag acatggcgaa
     1861 gagcgcagga aaggtcgagc agaaccggca gcaccgcatg cggcgcttcg ccgacgcgtc
     1921 gctccacatc gtcgccaccg aggggctcgc cgcgctgacg atggcccggc tggccgaaga
     1981 gctcgacacc gtgcccagcg cggtctaccg gtactttgcg tccaagggcg cgctcatcac
     2041 cgccgtgcag tgcgacgcga tcgaacgcct gaccgcgagc tacggcctca tccgcaccct
     2101 gagcgaggac cacttcgtcg agctcggcct cgacgaggcc gacaccgtgg tggcgcgtct
     2161 cgtcctgttc gggcgttggt tctgcgccac cgccgacacc catctcgagg agcttcgact
     2221 cctccagatg atcatgtccg agcgcaccca cgacctcgat ccgcagggcg gattcgaggt
     2281 gctcccgatc gccatggtcc tcctgggcca ggcggccgag tgcctcgacg ccgccgaggg
     2341 catcggcctg gcgacccccg gcgagtcgct cgagcgggtc atcgtgtggg ccgcagcgct
     2401 cggcggtgtg ctcgagaccg acagcctcgc ccgatacgtg ccggagctgt tgggcgacgg
     2461 tcggctcgcc cgtcgcacca acctcgacct gctccgcggg tggggtgtcc ccgacgatcg
     2521 gctcatcgcc gcgacccacg tggtcgacga tctggcgacg cgtgggagcc tcgcacccgc
     2581 cgtcgagcgg gcatcgtgac gctcgcccgg ctggtcggcg acgagctcac cgtcccctgc
     2641 atcgacggta ccgaccgcgt ctacctgggg ctcgacgccg gcgcgtcgac cgcggcgttc
     2701 gagtcggtcg cggcccgtgt ccaggagttc ctgccgtggt actcgagcgt gcaccggggt
     2761 gctggccgca agtcgcaggt ggccacccag gcctacgagg atgcccgcga ggcgatgctc
     2821 gagttcgcgg gccggggtga ctccgacgac gtcgccatct tctgccgcaa cacgaccgag
     2881 gcgatcaaca tcctggccta ccgactcgat gtcgagccgg gcgacacgat cgtgaccact
     2941 gtggccgagc atcacgccaa cctgctgccg tggcagcggc tggctcacga gcgcggcgtg
     3001 accatccgct acgtcgagtg cgacgagcgg ggggagttcg ccgccgagga cgtcgccgag
     3061 gcgatcgaga ccgccgatcg cccccgcctg ttggccatca cgggcgccac caacgtcacc
     3121 ggctggatgc ccccgctcgc cggcatcatc gaccacgcgc accggcacgg cgccgaggtg
     3181 ctcgtcgacg cggcgcagct cgcgccccac cgtcccctcg ccacgtcggg cgctgcggcc
     3241 gccgactatc tcgcgtggag tggccacaag atgtacgcgc ccttcggcac cggcgtgctc
     3301 atcggaccgc gccgcacgtt cgagacgggg acaccgctgc tcgtgggcgg cggtgctgtg
     3361 aacctcgtcg acctcgacga ggtgctgtgg accgatcccc ccgaacgcga cgaggccggc
     3421 tcgccgaacg tgctgggtgc ggtggcgctg cacgcggcgg tcgacgagtt cgcccgcatc
     3481 ggctgggacg ccatcgtcgc ccacgaggag gcgctcgcca cgcggctgcg ggtcggcctc
     3541 cgcgacctcg ccggagtccg ggtgctgggt gaggtccccg accgcgacga ccgtgactac
     3601 ctggctgtcg gctcgttcgt gatcggcacc gccgggtcgg gctccgccgg gccgctcccc
     3661 cacccgctcg tggcggcgcg gctcagcgcc gagcacgcca tcggggtgcg ccacgggtgc
     3721 ttctgcgccc acccctacct ggtgcgactg ctcggcctct ccaacgagga gacggcgcgt
     3781 taccgccacg acatcgtcgt cggcgaccgc cgcaacatcc ccggcgccgt tcgggccagc
     3841 gtgtcgatct cgacgaccga ggccgacgtc gatcggttcc tcgacgcgct cgatcgtgtc
     3901 atcagcggcc ccgaggcgcc ggtgcgctac cgacaggacc cgaccacggg cgactactgg
     3961 cccgtcccct gacgcaccga acagggccga acaggccgaa caggtccgcc gggtcagccg
     4021 ggtcagctca cttggcgctc aggtccgtga tgtaggcgac gacctgggcg agctcctcgt
     4081 cactgagacg gtcctcgtcg aaggtgggca tctggccggt cgcatcctcg cggcgctggg
     4141 cgttgggctc gcggacggcg cggacgaggt agtcggcgtc gacggtcgtg accgagccgt
     4201 ccttcaaggt gaccttcgaa ccgaggagac ccttccaggt ggggccggtc agctcgtccc
     4261 cgtcgaccga atggcagttc tggcactgga accgggcagc gaccgtcttg cccttcgtgc
     4321 cctccgcgga gagcttgacc ggcgggcccg aatcgtcggt gcacgcggac agggcggcgg
     4381 ggacggcgac ggtcagcaac gccgcgactg cgacgcgcac acgacgatgg acggagacgg
     4441 gtgagcgcat cggtccagta tcacccgtcg gtcgcggggg tcgcgcccac cagggtgccc
     4501 cggaatttcg gccctgcggc ggacccgccg ccgccacccg ccatccgcac gaaccgcacg
     4561 gcgatctgcg cccagatgac caggccaccg gccacctgga agatcgccgc ggccaactcc
     4621 tggtcggact gggcgtcgaa cgtgtcccac acccgcggcg ccagctcgta caccgagtag
     4681 atgggcgtcg gcgaccaggt catgaagaac gccaccggca gcggcgcgac cgacacgccg
     4741 atgagataga cgatcgccac cgggccccgc agtcgcggca ccatcggcga cggcggttgc
     4801 accggcatcc agatgaggaa cccggcgctg atccacgcca ggtcgagcgc aaacgagccg
     4861 agctgcgacg tcaccagcgg gtcgacgagg atcggcgcgc tcacggcgaa cagcaggctc
     4921 accgacacca cgagcgccag gagcgggtgg gtggcgatcc gcagcacggc acggcggcgg
     4981 ggtgtcgcct cgagccagcg gccgagcgat tcgggtgcac cgtagagcac gagcgggcac
     5041 gcgatcatca cgatcaacac ctgccggacc atctgcgccg acacgacgta ccccgcgccg
     5101 agcgtcgaca gcggccagtc ggtgccaccg aggatcagca cgaggccgag ggccagcgag
     5161 accttgcgcc gcgccgggac ctccgagcgg aacgcgcccg accgccagag ccacaccgcc
     5221 acagcggcca cgatggccca cgccccgatg tacggcttcc acgtccacga ccagttgctg
     5281 ccgtcgttcg agcaccacca tctcatgacg gatctccgac ccggctcatg acggatcccc
     5341 gaacccggct catcgcgtcg tcaccggcca ctcgtagtcg atgagccgcg ggagatcctt
     5401 cagccacgcc gccttgcgga tgccgaacgg atacacgatg tggcactcgt tgtcgggggt
     5461 gaacgccagg atctggctcg gatgttcgac cacgtaggag ccgtcgtcgc ggggctcgcc
     5521 gatgatgatc gacggcagct gcatcgaggc gagggcctcg tcgagctgtg ccggatcggc
     5581 gctcaggccg acgaaatcgg gattgcgctt gtcgagatag gtgcgcatca cctcgggggt
     5641 gtcgcgttgg gtgtcgacgc cgacgaagat gacgttggtc ttcgacgccg gaccggtgag
     5701 atccgccagt gtgtcggcga ggatgctcag gtggaccgga cagacatcgg ggcacgacgt
     5761 gtacccgaac agcatcagcg tgagcttgcc cacggtctcc gccgccacgt cgtagggggc
     5821 gccggacgtg tcggtgaacg cgatcgtcgg cttcgccacc gggtacccga gcaccgtgcc
     5881 ggcaaagccg aggttgtcgt cgtcgagcgc ggcgctgtcg tcgtcggagc aggccgtgac
     5941 gaggccggca ccggcggcga cgccgagcgc tgccaggccg gaggcccgga gaaacgagcg
     6001 gcgatcgtgc tcagccacgg ccgaccacgt catcgatgtc gtcggtcacc gtcgccggaa
     6061 ccgtcaccgt gccggcctgc tcgaacgtga gcgtgaggtc cacggtgtcg ccggcggcga
     6121 gccggtcggg ttgcagcatc atcaagtgga gccctccggg gctgagggag acggtctcgc
     6181 ccgccgggat cttgaccgat ccgacgcccg ccatggtggc gcggccgtcg ggatcgacgg
     6241 tggtctcgtg caacgtcgtc agctcggccc gggacgtgga gaccctcagg agccggtcat
     6301 cggcgtcgcc gtcgttggtg atcctcaggt agatcgccgc cgtgtcggcc gtggcaggga
     6361 cggggacgac ggcatcggcg acctcgatcc tcgacgtgcc gggcccgcac ccgaccagcg
     6421 cggccacgcc gaggctgccg acgagcggca ccacgaaccg gagggcggag gatcgacggg
     6481 gcacgtcgtc cagttcagcg cgccgggacc gtgccgccca agtcggcggt cgggcgtcac
     6541 atcacggcga cccgttctat cccgcgaacg cgcgctagaa cgcgcgcatc tacaggacag
     6601 aaccggtgac gtcggcgtgc agctcgggga ggaacgcggc gagatgggac atctcctgag
     6661 cgcagtggat gagcaggtcg gcggcgctct gagccgacgc tccgaggagc cgcgcaatca
     6721 cgggtcgggc ggcccggtca gccagcgccg cgcccggccg atcgccgcgg agcgcgatgt
     6781 gggagccacc catccagaaa cgcgaccgca tctcggcacc gtcgg
//
LOCUS       DAOSOA010000168         6780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11300_length_6780_cov_4.665130, whole genome shotgun sequence.
ACCESSION   DAOSOA010000168 DAOSOA010000000
VERSION     DAOSOA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11300_length_6780_cov_4.665130"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..829
                     /locus_tag="PKY13_10790"
                     /pseudo
     CDS             1..829
                     /locus_tag="PKY13_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611786.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            856..1392
                     /locus_tag="PKY13_10795"
     CDS             856..1392
                     /locus_tag="PKY13_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80466.1"
                     /translation="MQQSHLQGVEGRDTYKYLQHLRGVRLVSRRTSAGIAAVLALLTI
                     APGCSRSEDRSSEEYWYLDGQGRETLLIRSCWTDRIRVEIAPFDDGRYGPYRDLATQR
                     NGDEAGVWTAEIPEDVEHAERDHGQRYRIRVVADVDGSSTLVSAGGNADRSSIPSAPN
                     AVTFDENVVDTRSVGCTS"
     gene            1530..3998
                     /locus_tag="PKY13_10800"
     CDS             1530..3998
                     /locus_tag="PKY13_10800"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT80467.1"
                     /translation="MHRLAPSRRARTLLRAGLGATLVAVGLSVVPLPIVTAVTDYRVD
                     REPLRFEPMAAVQEAPSTDGEVISQVAEVEPFVTIGVALPEVPTSPVSVRWRDDEGWS
                     DWHELEVEVDKGPDQSTDEGAKAAEHREGVVTEPLWIGNADAYQVRLPEEVVDPAADT
                     STTTTSAPIDGAGPEVVVVRPDGQRLEVESDSVPAGAQSVGRPAIRTRGEWGARAPSN
                     SVGTSSSLKLAVVHHSATGNDYSPADVPGIIRSIQAFHIDGNGWSDIGYNFIVDKFGG
                     MWEGRDGSLLRNSIGAHAEGFNTGSVGVMALGNYTAGAPGAAQVTNIADVIAWKFANA
                     GIDPNTRVDFTSGGSSTVPAGQVVNLPRVVGHRDVGSTDCPGSNLYAQLSSRIRPILP
                     ALVAGKASPVGIVDVLAGGPRSVVLSGWTFDPLETDPIEVHVYIDGVGRNLGPTPYER
                     PDLATAYPGVGTRHGFATTIGDLSPGSHSVCVFGINTGPGSNTLLSCRDVVVQTGDPV
                     GNLDSVWAGPDGTVAVSGWALDPDQGAGIEIHAYVDGRGTNLGPAANYRPDIAGAFPG
                     YGGSHGFSWVTSGLGAGRHDVCVFAINVGAGSNILLGCRTVVAPGGAPVGVLDAVVGG
                     PDGALALWGWTLDPDTVGSTEVHVYVDGAGVNLGRASGDRPDIAGAFPGYGSRHGYSW
                     TTTGLAPGRHQVCAFAIDTGGGPNTLLRCTDATVPGGSPVGSIDAISGAGGRVGVVGW
                     TIDPDTSASIEAHVYIDGVGYNTGPAAQYRPDVAAVAPGYGAAHGISWMSPALAPGNH
                     SVCVFGINRGAGGNTLLTCRTVAV"
     gene            4084..5193
                     /locus_tag="PKY13_10805"
     CDS             4084..5193
                     /locus_tag="PKY13_10805"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOT80468.1"
                     /translation="MKGLILSGGAGTRLRPITHTSAKQLVPVANKPILFYGIEDMVAA
                     GITEIGIITGETGPEVRAAVGDGSAFGAHVTYIPQDEPLGLAHCVLIARDFLGDDDFV
                     MYLGDNMLQQGLVDFLARFHADRQAANELTLGDVGSEPPAAQILLCPVPDPHRFGIAE
                     VDADGHVVRLVEKPEDPPSDLALVGVYLFTKLIHEAVAAIEPSPRGELEITDAIQWLI
                     ENGHRVRHDLLDGWWLDTGKKDPLLESNRRVLEELEQRIDGELDESSEIDGRVVIEAG
                     AKLVNSHVRGPAIIGAGTTLTNSYVGPFTSVAAGCAISDSEVENSVILERARISGVPR
                     LVDSLIGRDTHVHRTEQRPKATRLMIGDDCSIDLE"
     gene            5213..5779
                     /gene="rfbC"
                     /locus_tag="PKY13_10810"
     CDS             5213..5779
                     /gene="rfbC"
                     /locus_tag="PKY13_10810"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOT80469.1"
                     /translation="MATITESDVIAGVQIVEPKKFGDERGYFIENYRREWFPQGREMI
                     QGNRGNRIAGCLVGLHYHLHQADYWYVPFGHARVVLHDLRQGSPTDGATQVLDLGEVD
                     GGENNHLGIFIPPGVAHGFASLSDMAITYLVDGYYNPADENGVAWNDPAVDADWGLTD
                     PILSARDQANPLRTELDPMFQPHYQLRP"
     gene            5793..6761
                     /gene="rfbB"
                     /locus_tag="PKY13_10815"
     CDS             5793..6761
                     /gene="rfbB"
                     /locus_tag="PKY13_10815"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOT80470.1"
                     /translation="MKLFITGGAGFIGSNFARHILTNTDDSVVVFDALTYAGNRENLA
                     DLEDDPRFSFVHGNICDRDAVRAAMDGCDQVVHFAAESHVDRSLLDPDVFVRTNCDGT
                     NVMCDIATQIGVERFLHISTDEVYGSIEEGSFVETDALHPRSPYSSAKAGSDLIAMSY
                     HETYDLPVVITRSSNQFGPYQFPEKVIPFFVTTLLEGGKVPLYGDGLNVRDWLFVLDN
                     CTGVQTVLRDGKVGEVYNIGAGNEKTNREITDTILAALGKDESSVEYVEDRKGHDRRY
                     SIATDKSAALGWAPSRTFEQAIDDTIKWYVDHRDWWAPLRERVKNR"
BASE COUNT         1126 a         2380 c         2177 g         1097 t
ORIGIN      
        1 tcacggtgac gtgtcgggtc ctcaaccttg cccgccagcc ctactaccgg tggctcctgg
       61 agcccttcac tgacgccgag ttgaacgagg cgtggctggc gaacgcgatc ttcgacgcgc
      121 atcgcgacga tcccgagttc gggtatcggt tcttgtgcga cgaagttcgc gatgccggcc
      181 acacggtgtg tgaacggacg gtgtggcgga tctgccgtga catgggctgg tggtcggtgt
      241 tcggcaagaa gcgtggctcg aagaagtcca aggtcggcgc ccccgcccac gatgacctgg
      301 tgcgccgcga gttcaccgcg gatcggccga accggctgtg gctcacggac ctcacggagc
      361 attggacgaa cgaaggcaag ctctacctct gcgccatcaa ggacgcgtag tcgcggcgga
      421 tcgtcggcta ctccatcagc gaccggatgc aggcccggat cgcggtcgcg gcgctcgaca
      481 acgctgtcgc ccgccgtggc gatgtcgccg gttgcatcct gcattcggac cgtggatcgc
      541 atttcgatcc cggaaactcc acagtgcgct cgcccgccac gacatggtcg ggtcgatggg
      601 ccaggtcggt tcagccggcg acaacgcggc gatggaatcg ttcttcgcgc tcttgcagaa
      661 gaacgtcctc gatcgccaac gctgggcgac ccgcgacgag ttgcggatcg cgatcgtcac
      721 ctggatcgag cgcacctacc accgccgacg gcgtcaagcc gccctcggcc ggttgacccc
      781 gatcgagttc gaaacgatca tgaccaaccc cgccacccag gcggcctaac cccaaacccc
      841 acctgtcacc cgatcttgca gcagtcccat ctgcaaggcg ttgaaggccg cgacacgtac
      901 aaatacctac aacatcttcg gggagtcaga ttggtgagtc gacgaacaag cgccggtatt
      961 gcggcggtac tcgctctact gaccatcgca ccgggctgct cccggtcgga ggatcgctcc
     1021 agcgaggagt attggtacct ggacgggcaa ggccgcgaga cgttgctcat ccgttcgtgc
     1081 tggaccgatc ggatacgcgt cgagatcgct ccgttcgacg acggacggta cgggccgtac
     1141 cgcgacctcg ccacgcaacg caacggtgac gaggcaggag tgtggacggc ggagataccg
     1201 gaggacgtgg aacatgccga acgcgaccat ggccagcgat atcggatcag agtcgtggcc
     1261 gatgttgacg gcagctcgac gttggtcagc gcgggtggaa acgccgaccg atcctcgatt
     1321 ccgtccgcgc cgaacgcggt gaccttcgac gagaacgttg tcgacacccg atccgtcgga
     1381 tgtacttcct gactgctcag cgctctcgaa cggatccaac cgggcgcagg tggggcgagt
     1441 tcccgaccgc ccgcggcaat cggcctgcca aggactcggc ggccccggct cctacagtgt
     1501 cgcggccgct tccgaaggat ccttccctca tgcaccgtct cgccccgtcc cgccgcgccc
     1561 gcaccttgct tcgtgccggg ctcggtgcca ccctggttgc cgtcgggttg tccgtcgtcc
     1621 cgctgccgat cgtcaccgcc gtcaccgact accgcgtcga ccgcgaaccg ctccgattcg
     1681 agcccatggc cgcggtccag gaagcgccgt ccaccgacgg cgaggtcatc tctcaggtgg
     1741 ccgaggtcga gccgttcgtg acgatcgggg tcgcgttgcc cgaggtcccg acgtcaccgg
     1801 tgtcggtgcg ctggcgcgac gacgagggct ggtccgactg gcacgagctc gaggtcgagg
     1861 tcgacaaggg ccccgaccag tccaccgacg aaggggcgaa ggccgccgag caccgtgaag
     1921 gtgtcgtcac cgagcccctg tggattggca acgccgacgc ctaccaggtg cgcctccccg
     1981 aggaggtggt ggatccggca gccgacacgt cgacgaccac aacgagcgct cccatcgacg
     2041 gagcggggcc tgaggtcgtc gtcgtgcggc ccgacggcca acggctggag gtggagtccg
     2101 acagcgtgcc cgccggagcg cagagcgtcg gccggcccgc cattcgtacc cgcggcgagt
     2161 ggggcgcccg ggcaccgtcc aactcggtcg gtacttcgtc gtccctgaag ctcgccgtag
     2221 tgcaccactc ggcgacgggc aacgactact cgccggccga cgtgcccggc atcatccgat
     2281 cgatccaggc gttccacatc gacggcaacg gctggtccga catcgggtac aacttcatcg
     2341 tcgacaagtt cggtggcatg tgggaaggcc gcgacggcag cctgcttcgc aacagcatcg
     2401 gcgcccacgc cgagggcttc aacacgggct cggtcggtgt gatggccctc ggcaactaca
     2461 ccgccggtgc cccgggcgcg gcccaggtca ccaatatcgc cgacgtcatc gcctggaagt
     2521 tcgcgaacgc cggcatcgac cccaacaccc gcgtcgactt cacgtcgggc ggcagctcga
     2581 ccgtccccgc cggccaggtc gtgaaccttc cccgcgtcgt cggccaccgc gacgtgggct
     2641 cgaccgactg cccgggttcc aacctgtacg cacagctgtc gtcacgcatc cgtccgatcc
     2701 tcccggcgct ggtcgccggc aaggcgtcgc ccgtcggcat cgtcgacgta ctggccgggg
     2761 gaccgcgctc ggtggtgctg tcgggttgga cgttcgatcc actcgagacc gatcccatcg
     2821 aggtgcacgt gtacatcgac ggcgtcggcc gcaacctcgg cccgaccccg tacgagcggc
     2881 cggaccttgc gacggcctac cccggggtcg gcaccaggca tggctttgcg acgaccatcg
     2941 gcgatctctc gccgggaagc cacagcgtgt gcgtgttcgg catcaatacg ggaccgggct
     3001 ccaacacgct gctgtcgtgc cgcgatgtcg tggtgcagac gggtgatccc gtgggcaacc
     3061 tcgacagtgt ctgggccggc cccgacggca cggttgccgt gagtggttgg gcgctcgatc
     3121 ctgaccaggg cgccgggatc gagatccacg cctacgtcga cggacgggga acgaacctcg
     3181 gcccggcagc gaactaccga cctgacatcg ccggcgcgtt ccccggctac ggcgggtcgc
     3241 acgggttctc gtgggtcaca tcaggcctcg gtgccggacg ccatgacgtg tgcgtgttcg
     3301 ccatcaacgt cggagccggg tccaacatcc tcctgggatg tcgcacggtc gtcgcgcccg
     3361 gcggtgcccc ggtcggtgtg ctcgacgcgg tggtcggcgg gcccgacggg gcactggccc
     3421 tgtggggttg gacgctcgat cccgacacgg tgggttcaac ggaggtccat gtctacgtcg
     3481 acggcgccgg cgtcaacctc ggccgcgcgt cgggcgatcg ccctgacatc gcgggagcgt
     3541 tccccggcta tggctcgcgc cacgggtact cgtggacgac gacgggactg gcgccgggcc
     3601 gccatcaggt ctgcgcgttc gccatcgaca ccggcggcgg tcccaacacc ttgctccgct
     3661 gcaccgacgc gaccgtcccc ggcggttcgc cggtcgggtc gatcgacgcg atctccgggg
     3721 ccggcggccg ggtcggtgtg gtcggctgga cgatcgaccc ggacacgtcc gcatcgatcg
     3781 aagcgcacgt ctacatcgat ggtgttggct acaacaccgg acctgcggcg cagtaccgcc
     3841 ccgatgtcgc ggcggtggct ccgggctacg gcgccgctca cggcatctcg tggatgtcgc
     3901 cggcgctcgc accgggcaac cactcggttt gtgtgttcgg tatcaaccgc ggtgccggtg
     3961 gcaacacgct cttgacctgc cggacggtcg ccgtctgacc gttcccgagc gctggtcgga
     4021 gcccggtccc gccatggtcc gggctgcgtc tcgacactgg atcgctgcta gacagacacc
     4081 actgtgaagg gcctcattct ctccggcgga gcgggcaccc gcctgcgacc gatcacccac
     4141 accagcgcca agcagctggt gccggtggcc aacaagccga tcctgttcta cggcatcgag
     4201 gacatggtgg ccgccggaat caccgagatc ggcatcatca ccggtgagac cggtcccgag
     4261 gtgcgggcgg cggtgggtga cggctcggcg ttcggagcac acgtcaccta cattccccag
     4321 gacgagccgc tcgggttggc gcactgcgtc ctcatcgccc gtgatttcct gggtgacgac
     4381 gacttcgtca tgtacctcgg cgacaacatg ttgcagcagg gtctggtcga cttcctcgcc
     4441 cgcttccacg ccgaccgtca ggcggccaac gaactgacgt tgggcgatgt tggttcggag
     4501 cctccggccg cgcagatcct gctctgtccg gtgccggatc cccatcgttt cgggatcgcc
     4561 gaggtggacg ccgacggtca cgtcgtgcga ttggtcgaga agcccgagga cccgccgtcg
     4621 gatctggcgc tcgtcggggt gtacctcttc accaagctca tccacgaagc ggtcgcggcg
     4681 atcgagccgt cgccccgtgg cgagctcgag atcaccgacg ccatccagtg gttgatcgag
     4741 aacgggcatc gggtacgcca cgacctgctc gacggctggt ggctcgacac cggcaagaag
     4801 gacccgctgc tcgaatcgaa ccggcgggtg ctcgaagagc tcgagcagcg catcgacggc
     4861 gagctcgacg agtcgagcga gatcgacggc cgcgtggtga tcgaggccgg agcgaagctc
     4921 gtgaacagcc acgtgcgcgg tcccgccatc atcggcgccg gcaccaccct gaccaacagc
     4981 tacgtcggcc cgttcacctc ggtcgccgcc ggctgcgcca tcagcgattc cgaggtcgag
     5041 aactccgtga tcctcgaacg tgcccgcatc tcgggcgttc cccgcctcgt cgactcgttg
     5101 atcggccgcg acacccacgt gcaccggacc gagcagcgcc ccaaggccac ccgcctgatg
     5161 atcggcgacg actgctccat cgacctcgaa tagcgagaca ggtagatctt ccatggccac
     5221 catcaccgaa tccgacgtca tcgccggcgt acagatcgtc gagccgaaga agttcggcga
     5281 cgagcgcggg tacttcatcg agaactaccg ccgcgagtgg ttcccccagg gtcgcgagat
     5341 gatccagggc aaccgcggca accgcatcgc cgggtgcctc gttggcctgc actaccacct
     5401 gcaccaggcc gactactggt acgtcccgtt cgggcatgcc cgcgtcgtcc tgcacgacct
     5461 ccgtcagggc tcgcccaccg acggcgccac gcaggtcctc gacctcggcg aggtcgacgg
     5521 tggcgagaac aaccacctcg gcatcttcat cccgcccggt gtcgcccacg gcttcgcctc
     5581 gctgtcggac atggcgatca cctacctcgt cgacggctac tacaaccccg ccgacgagaa
     5641 cggcgtggcg tggaacgatc ctgcggtcga cgccgactgg ggtctcaccg acccgatcct
     5701 gtcggctcgc gaccaggcca acccgctgcg caccgagctg gacccgatgt tccagccgca
     5761 ctaccaactc cgaccctagg atctccccaa tcatgaagct gttcatcacg ggcggcgcgg
     5821 ggttcatcgg ttcgaacttc gcgcgccaca tcctcaccaa caccgacgac tcggtggtcg
     5881 tcttcgacgc cctcacctat gccggaaacc gcgagaacct cgccgatctc gaggacgacc
     5941 cacggttctc gttcgtgcac ggcaacatct gtgaccgcga cgccgtccgt gctgccatgg
     6001 acgggtgcga tcaggttgtg cacttcgcgg ccgagagcca cgtcgatcgt tcgctcctcg
     6061 accccgacgt cttcgtccgc accaactgcg acggcaccaa cgtcatgtgc gacatcgcca
     6121 cccagatcgg cgtcgagcgc ttcctgcaca tctccaccga cgaggtgtac ggctccatcg
     6181 aagagggcag cttcgtcgag accgacgccc tgcacccgcg ttcgccgtac tcgtcggcga
     6241 aagcagggtc cgacctgatc gccatgtcgt accacgagac ctacgacctc ccggtggtca
     6301 tcacccggtc gtccaaccag ttcgggccgt accagttccc cgagaaggtc atcccgttct
     6361 tcgtcacgac gctgctcgag ggcggcaagg tgccgctcta cggcgacggt ctcaacgtcc
     6421 gcgactggtt gttcgtgctg gacaactgca ccggcgtgca gaccgtgctg cgcgacggca
     6481 aggtcggtga ggtctacaac atcggcgccg gcaacgagaa gaccaaccgc gagatcaccg
     6541 acacgatcct cgccgccctc ggcaaggacg agtcgagcgt cgagtacgtc gaggatcgca
     6601 agggtcacga tcgccgctac tcgatcgcca ccgacaagag cgcagccctc ggctgggcgc
     6661 cgagccgaac cttcgagcaa gcaatcgacg acacgatcaa gtggtacgtc gaccaccgcg
     6721 actggtgggc accgctccgc gagcgggtca agaaccgctg atcatgcgaa tcctgatcac
//
LOCUS       DAOSOA010000169         6714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11444_length_6714_cov_5.569755, whole genome shotgun sequence.
ACCESSION   DAOSOA010000169 DAOSOA010000000
VERSION     DAOSOA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11444_length_6714_cov_5.569755"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /gene="hisF"
                     /locus_tag="PKY13_10820"
     CDS             complement(<1..726)
                     /gene="hisF"
                     /locus_tag="PKY13_10820"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOT80471.1"
                     /translation="MNVTRVIPCLDVDAGRVVKGTNFVNLVDAGDPVELAARYDAEGA
                     DELVFLDITASSDRRDTTIDMVARVAEEVFIPFTVGGGVRSVADARALLRAGADKVGV
                     NTAAVERPELVSEIAAEFGAQCVVVAVDSRHTGATPSGWEVFVHGGRTPTGVDVLEWV
                     ERVAALGAGEILLTSMDRDGTKDGFDVEVTRAVAERVNVPVIASGGVGTLDHLVRGVT
                     EGGADAVLAASIFHFGEFTVAEAK"
     gene            complement(723..1472)
                     /gene="hisA"
                     /locus_tag="PKY13_10825"
     CDS             complement(723..1472)
                     /gene="hisA"
                     /locus_tag="PKY13_10825"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOT80472.1"
                     /translation="MIDLYPAIDLRGGRCVRLYQGDYDRETHYGDDPVSQARAFADAG
                     APWIHVVDLDAARSGEATNREVIAAICAAVDVPVQSGGGVRTVDAAAALADAGVSRVV
                     IGTAALEQPELVAAVAARQPVAVGLDARGSDLAAHGWEVSSGLDLTTVATRFADAGVA
                     AFVVTEIARDGTLEGPSIEQLSSVLAALSPFGADAPGVIASGGVGTVDHVTALDALAV
                     DGSRLAGVIMGRALYEGAFTLTEALSAVGAR"
     gene            complement(1486..2106)
                     /gene="hisH"
                     /locus_tag="PKY13_10830"
     CDS             complement(1486..2106)
                     /gene="hisH"
                     /locus_tag="PKY13_10830"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOT80473.1"
                     /translation="MVLVKLAVLDYGIGNLRSAQKAFEHVGADARLTADLGDIEAADA
                     VVLPGVGAFGACMDALRSCGLDTVALDVVASGRPFMGICVGMQMMYASSDEDPGAEGL
                     AIFDLPIHLLPATVKRPQMQWNRLVAKDPDHPMLRGLVDPWVYFVHSYAAEGGDEVVA
                     TCDYGIDVTAAVARDNVWATQFHPEKSADAGLTILRNFLTWAGEQT"
     assembly_gap    2116..2215
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2217..2807)
                     /gene="hisB"
                     /locus_tag="PKY13_10835"
     CDS             complement(2217..2807)
                     /gene="hisB"
                     /locus_tag="PKY13_10835"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOT80474.1"
                     /translation="MTRSASRSRTTKETDISITVEVDGDGTNDIATGLPFFDHMLDQL
                     GRHGGMNLTVHATGDLHVDGHHTVEDVGILLGETFREALGDKAGVRRFASGLFPLDEA
                     LIEVALDLSGRPYLVYDVPFGEVLPLGTPPFNPEMAEHFFRSFVDAAHVTLHVTRKGG
                     TNTHHVIEATFKGVARCIRDAVRVEGTALPSTKGVL"
     gene            complement(2804..3880)
                     /gene="hisC"
                     /locus_tag="PKY13_10840"
     CDS             complement(2804..3880)
                     /gene="hisC"
                     /locus_tag="PKY13_10840"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT80475.1"
                     /translation="MARPQVRDDIAMMSGYHSPQLDVAIRLNTNEAPEPPPAEFRERV
                     AAATADIAWHRYPSRDAAGLRARIAEVEGVAPAQVFAANGSNEVLQTVMLAYGGAGRT
                     ALTFEPTYALHGHISRLVGTRVVEGERTDDFGLDLDVVARLIESERPAVTFLCSPNNP
                     TGVIETEATIRTVLDLVHAVDGLLVVDEAYGQFAPWSALELVDDANSLVVSRTYSKTW
                     SMAAARLGYGIAPAWVVEELDKVVLPYHLDAAKQIAGTIALDYLDEMRARVATLVEER
                     GRVAAALDDLDVDHWPSGANFILLRPRHRPGQEVWQDLVDRSILVRNCASWPRLDDCL
                     RLTLGTPDENTEFLAALAASLEPS"
     gene            complement(3880..5193)
                     /gene="hisD"
                     /locus_tag="PKY13_10845"
     CDS             complement(3880..5193)
                     /gene="hisD"
                     /locus_tag="PKY13_10845"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOT80476.1"
                     /translation="MLNRLDLRAIEGDVTAHLPRPDVASEGPVAAVRSILAAVRADGD
                     AALHRYTKEFDGIDLDTLRVDPAEIAAALDRIPADVRDALHVAAGRIEAFHRTQLHEP
                     TSYVDGGTIRSFAVPVDRAGCYVPGGRAAYPSTVLMTAIPAKVAGVGQVVVCVPPDRT
                     TGAIVDVTLAACAVAGVDEVYAIGGAQAIGALAYGTESVAKVDVICGPGNKYVAIAKR
                     EVAEVVGIASAFAGPSEIVVVADHSAPPSFAAVDVVVQAEHGPDGLAWLVCWDDTVAD
                     AVSAEVARITTESPRRAEIESTLAVAGYAVVCRDEAQAMEVANAIAPEHLQLMTSDPE
                     ALVPSVRNAGAIFCGPWSPASLGDYVAGPSHVLPTDGTARFSSALTVGDFQKHHHVIT
                     FSPAEAAALAPAVEAFATAEGLDAHALSARLRREAFEGTTGEGSR"
     gene            5259..5456
                     /locus_tag="PKY13_10850"
     CDS             5259..5456
                     /locus_tag="PKY13_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80477.1"
                     /translation="MSSAAELSSAASSLDQLVHRIGRAVDEFTGSNDEDLTLGLMEVE
                     RSLKTASRRLERLVKELRARQ"
     gene            complement(5825..6475)
                     /gene="nth"
                     /locus_tag="PKY13_10855"
     CDS             complement(5825..6475)
                     /gene="nth"
                     /locus_tag="PKY13_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOT80478.1"
                     /translation="MARPRSPKGRARLTHERLAVEYPEAECELDHTNAFELLAATILS
                     AQSTDQRVNMVTPELFRRWPTPAALSVAPPEEVQEVIHSTGFFQSKTKSLLGMATRLV
                     EEFGGEVPGAMADLVSLPGVGRKTANVVRSVALGLPGLPVDTHVLRLTKRLGIIEPTD
                     TDPVKVELLLNPMVPEAERGEFSLRIILHGRRVCFARKPNCGGCALADFCPSAGAA"
     gene            6498..>6714
                     /locus_tag="PKY13_10860"
     CDS             6498..>6714
                     /locus_tag="PKY13_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80479.1"
                     /translation="MTSTGPRPGHPTEAELADAIERHGGRLDESQGRPRWWITDPSSA
                     AEDTATHIGLAEHRRLFQMRRPLPLDLT"
BASE COUNT         1019 a         2239 c         2417 g          939 t
ORIGIN      
        1 cttggcctcg gcgacggtga actcgccgaa gtggaagatc gacgcggcga ggaccgcgtc
       61 ggcgccgccc tcggtgacgc cacgcacgag gtggtcgagc gtgccgacgc cgcccgaggc
      121 gatgacgggg acgttcaccc gttcggcgac ggctcgggtg acctcgacgt cgaagccgtc
      181 cttggtgccg tcgcggtcca tcgaggtgag caggatctcc cccgccccga gcgcggcgac
      241 gcgttcgacc cactcgagca cgtcgacccc ggtgggcgtg cgtccgccgt gcacgaacac
      301 ctcccagccc gacggcgtgg cgccggtgtg acgggaatcg acggcgacga ccacgcactg
      361 ggcgccgaac tcggcggcga tctcggaaac gagctcgggc cgctcgacgg cggccgtgtt
      421 cacccccacc ttgtcggcgc cggcgcgaag gagggcacgg gcgtcggcga ccgaacgcac
      481 cccgccaccg acggtgaacg ggatgaacac ctcctcggca acccgggcca ccatgtcgat
      541 ggtcgtgtcg cgacggtccg agctagcggt gatgtcgagg aacaccagct cgtcggcgcc
      601 ctcggcgtca tagcgagccg ccagctcgac gggatcgccg gcgtcgacga ggttcacgaa
      661 gttggtgccc ttcacgacgc gcccggcgtc gacgtcgagg cacgggatga cccgggtgac
      721 gttcatcgcg cccccaccgc cgacagtgcc tcggtcaggg tgaacgcccc ctcgtacagg
      781 gctcggccca tgatgacgcc ggcgagtcgg gagccgtcca cggcgagggc gtccagcgcg
      841 gtgacgtgat cgaccgtgcc gacaccgcct gatgcgatca cgcccggggc gtcggcgccg
      901 aagggtgaca gcgccgccag cacggagctc agctgttcga tcgacggacc ttcgagggtg
      961 ccgtcgcggg cgatctcggt caccacgaac gccgcgacac cggcatcggc gaaccgggtg
     1021 gcgacggtcg tcaggtcgag accggagctg acctcccagc cgtgcgcggc gaggtcactg
     1081 ccgcgggcgt cgaggccgac cgccacgggt tggcgggcgg cgacggcggc gaccagttcg
     1141 ggttgttcga gggcggccgt gccgatgacg acccggctca cgccggcgtc ggccagcgcc
     1201 gcggcggcgt cgacggtgcg gacgccaccg cccgactgca cgggcacgtc gaccgctgcg
     1261 cagatggcgg cgatcacctc acgattggtg gcctcgcccg agcgtgcggc gtcgaggtcg
     1321 acgacgtgga tccagggcgc tccggcgtcg gcgaaggcac gggcctgact cacggggtcg
     1381 tcgccgtagt gcgtttcgcg gtcgtagtcg ccctggtaga ggcggacaca gcggccgcca
     1441 cgcaggtcga tggcggggta cagatcgatc atggcgtcct tcgggtcacg tctgctcgcc
     1501 ggcccaggtg aggaagttgc ggaggatggt gaggccggcg tcggccgact tctcggggtg
     1561 gaactgggtg gcccacacgt tgtcgcgggc gacggcagca gtgacgtcga tgccgtagtc
     1621 gcaggtggcg acgacctcgt cgccgccttc cgcggcgtag ctgtgcacga agtagaccca
     1681 cgggtcgacg aggccgcgca gcatcgggtg gtcgggatcc tttgcgacca ggcggttcca
     1741 ctgcatctgc gggcgcttga ccgtggccgg caggaggtgg atcggcagat cgaagatcgc
     1801 gagcccctcg gcgcccgggt cctcgtcgga gctggcgtac atcatctgca tgccgacgca
     1861 gatgcccatg aacgggcggc cggacgccac gacgtcgagt gcgacggtgt cgaggccaca
     1921 cgagcgaagc gcgtccatgc aggcgccgaa cgctccgact ccggggagga cgacagcgtc
     1981 ggcggcttcg atgtcgccga ggtcggcggt gagtcgggcg tcggcgccga cgtgttcgaa
     2041 cgccttctgc gccgaccgca ggttgccgat gccgtagtcg aggacggcca gcttcacgag
     2101 caccacccca ccctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcac
     2221 aggacgccct tggtggacgg gagggccgtg ccctcgacgc ggacggcgtc gcggatgcat
     2281 cgcgccacgc ccttgaatgt cgcctcgatc acgtggtgcg tgttcgtgcc ccccttgcgg
     2341 gtcacgtgga gcgtgacgtg ggcggcgtcg acgaacgagc ggaagaagtg ctcggccatc
     2401 tcggggttga acggcggggt cccgagcggc agcacctcac cgaacggcac gtcgtagacg
     2461 aggtacgggc ggcccgacag gtcgagcgcg acctcgatca gcgcctcgtc gagcgggaag
     2521 agaccggacg cgaaccgacg caccccggcc ttgtcgccga gcgcctcgcg gaacgtctca
     2581 ccgagcagga tgccgacgtc ctcgacggtg tggtgcccgt cgacgtgaag gtcaccggtg
     2641 gcgtgcaccg tcaggttcat gccgccgtgc cgaccgagct ggtcgagcat gtggtcgaag
     2701 aacgggagtc cggtggcgat gtcgttggtg ccgtcaccgt cgacctcgac cgtgatcgag
     2761 atgtcggttt ccttggtggt acggctacgg gaggcactgc gtgtcatgag ggctccaggc
     2821 tcgcagccag cgctgcgagg aactcggtgt tctcgtcggg ggtgccgagc gtgaggcgaa
     2881 gacagtcgtc gaggcggggc cacgacgcgc agttgcgcac caggatcgag cggtcgacga
     2941 gatcctgcca gacctcctgg ccggggcggt gacgaggacg caggaggatg aagttggcgc
     3001 ccgacggcca gtggtcgacg tcgaggtcgt cgagcgccgc tgccacccgg ccgcgttcct
     3061 cgaccagcgt ggcgacgcgg gcccgcatct cgtcgaggta gtcgagggcg atggtgccgg
     3121 cgatctgctt ggcggcgtcg aggtggtacg gcagcaccac cttgtcgagt tcctcgacga
     3181 cccaggccgg cgcaatgccg tacccgagtc gggcagccgc catcgaccag gtcttggagt
     3241 acgtgcgcga cacgacgagc gagttggcgt cgtcgacgag ctcgagcgcc gaccacgggg
     3301 cgaactggcc gtacgcctcg tcgacgacga ggaggccgtc gacggcgtgc accaggtcga
     3361 gcacggtgcg gatcgtcgcc tcggtctcga tgaccccggt cgggttgttg ggcgagcaca
     3421 ggaacgtgac ggcgggccgc tcggactcga tgagccgggc gacgacgtcg aggtcgagac
     3481 cgaagtcgtc ggtgcgctcc ccctccacga cacgagtgcc caccaaccgg gagatgtgac
     3541 cgtggagcgc gtacgtgggt tcgaacgtga gggcggtgcg accggctccg ccatacgcca
     3601 gcatgacggt ctgcagcacc tcgttcgacc cgttggccgc gaacacctgg gctggcgcga
     3661 cgccctcgac ctcggcgatg cgagcgcgca ggccggcagc gtcgcggctc gggtagcggt
     3721 gccaggcgat gtcggcggtc gccgcggcga cacgctcgcg gaactcggcg ggtggtggct
     3781 ctggtgcctc gttggtgttg aggcgaatgg cgacgtcgag ctggggcgag tggtagcccg
     3841 acatcatcgc gatgtcgtcg cggacctggg ggcgggccat cagcgggacc cctcgccggt
     3901 cgtgccctcg aacgcctcgc gacgcaggcg ggccgacagc gcgtgggcgt cgaggccctc
     3961 ggccgtcgcg aacgcctcga cggcgggagc caacgctgcg gcctcggccg gcgagaaggt
     4021 gatgacgtgg tggtgcttct ggaagtcgcc gacggtgagc gccgagctga accgggccgt
     4081 gccgtcggtg gggagcacgt gcgacggccc ggccacgtag tcacccaacg atgcgggtga
     4141 ccagggtccg cagaagatcg cgccggcgtt ccggaccgac ggcaccaggg cttcggggtc
     4201 ggaggtcatc agctgcaggt gctcgggggc gatggcgttg gcgacctcca tggcctgcgc
     4261 ctcgtcacgg cacacgacgg cgtacccggc gacagcgagg gtcgactcga tctcggcgcg
     4321 ccgcggcgac tcggtggtga tgcgggccac ctcggcgctg acggcgtcgg cgaccgtatc
     4381 gtcccaacag accagccagg cgagcccgtc gggcccgtgc tcggcctgca cgacaacgtc
     4441 gacggcggcg aagctcgggg gtgcggagtg atctgccacc acgacgatct cgctcgggcc
     4501 ggcgaaggcc gaggcgatgc ccacgacctc ggcgacctcg cgcttggcga tggcgacgta
     4561 cttgttgccg ggaccacaga tgacgtcgac cttggcgacc gattcggtgc cgtaggccaa
     4621 cgccccgatc gcctgcgcac cgccgatggc gtacacctcg tcgacgccgg cgaccgcgca
     4681 cgcggccagg gtcacgtcga cgatcgcccc ggtcgtccgg tcggggggaa cgcacacgac
     4741 gacctgaccg accccggcca ccttggcggg gatcgcggtc atgagcacgg tcgacggata
     4801 ggcggcccgc ccacccggca cgtagcaccc ggcccggtcg accggcacgg cgaagctccg
     4861 gatcgtgccg ccgtcgacgt agctcgtcgg ctcgtgcagc tgggtgcggt ggaacgcctc
     4921 gatgcggccg gcggcgacgt ggagggcgtc gcggacatcg gcggggatcc ggtcgagcgc
     4981 cgcagcgatc tcggcagggt cgacacggag ggtgtcgagg tcgatgccgt cgaactcctt
     5041 cgtgtaccgg tggagcgcgg cgtcgccgtc ggcacggacg gcagcgagga tcgagcgcac
     5101 ggcagcgacc gggccctccg atgcgacgtc gggccggggc aggtgggcgg tcacgtcgcc
     5161 ctcgatggcg cgcaggtcga gacggttcaa cacggtccgc acgctaccgt ccgcccgaac
     5221 ggcggcccga atcagttacc gctgctagac aggcggagat gagctcagcc gccgaactct
     5281 cctccgccgc gtcctcgctg gaccagctgg tgcaccgcat cggtcgggcc gtcgacgagt
     5341 tcaccggcag caacgacgag gatctcaccc tcgggctcat ggaggtggag cgctcgctca
     5401 agacggcgag ccgccggctc gaacgcctcg tcaaagagct tcgcgcccgc cagtagcgcg
     5461 cctcgcttcc gtcacggcgg ctcgcgaacg ctgcgtttcg gtggtgcgcg tcacggctcg
     5521 agcagggtcg aacttgccgg aaaacaagga ccggcgccac cacgaagggg cgccggtcgg
     5581 gcgaaacggg tggcgggatc ccattgtcgc cggttccggg tggcggacac ccggattgcg
     5641 gaacaactta cagggcccaa cgaccactgt ccagtgtttc cgtaccactc ttcgtgacgt
     5701 ctggagacaa ttcgcaccat attagtgcga ctcccttaac tgtaaccccc aaagagttcg
     5761 tgaaggattg cccaagagcg cgagaacgac gttctggttc gcgacagatt gtcgcgaacg
     5821 gggttcaggc cgctccggcc gacggacaga agtcggcgag cgcacaccca ccgcagttgg
     5881 gtttgcgggc aaagcagacc cgacgcccgt ggaggatgat gcgcaggctg aactcgcccc
     5941 gctccgcctc gggcaccatc gggttgagga ggagttcgac cttgaccgga tcggtgtcgg
     6001 tcggctcgat gatgcccagc cgcttcgtga gccggagcac gtgggtgtcg accggcaacc
     6061 ccggcaggcc caacgccacg ctccgcacga cgttggccgt ctttcgcccg acgcccggca
     6121 acgacacgag gtcggccatc gcccccggca cctccccacc gaactcctcg acgagccggg
     6181 tggccatgcc gagcaggctc ttcgtcttcg actggaagaa ccccgtcgag tggatgacct
     6241 cctgcacctc ctcgggaggt gctaccgaca gtgccgccgg cgtgggccac cgacggaaca
     6301 gctccggggt gaccatgttg acccgctggt cggtgctctg cgccgagagg atcgtggcgg
     6361 cgagcagctc gaaggcgttc gtatggtcga gttcgcactc ggcctcgggg tattcgacgg
     6421 cgagccgctc gtgggtgaga cgggcacgcc ccttcggcga tcgcggtcgg gccatgcggt
     6481 gaacgtagcc tgtccccatg acatcgaccg gcccccgccc gggccacccg accgaggccg
     6541 agctcgccga cgccatcgaa cgccacggcg gccgactcga cgagtcccag ggccgtcccc
     6601 gttggtggat caccgacccg tcgtccgccg ccgaggacac cgcaacgcac atcggcctgg
     6661 ccgagcaccg ccgcctcttc cagatgcgac gcccgctccc cctcgacctc acac
//
LOCUS       DAOSOA010000170         6654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11591_length_6654_cov_4.238369, whole genome shotgun sequence.
ACCESSION   DAOSOA010000170 DAOSOA010000000
VERSION     DAOSOA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11591_length_6654_cov_4.238369"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /locus_tag="PKY13_10865"
     CDS             complement(<1..1901)
                     /locus_tag="PKY13_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80480.1"
                     /translation="MTAEAATSPDVVLVVRQLREHHPAATITVVVTHPLQLAAPVPGA
                     TSTVSARHLTVAGVRYIDAWLGGGPAFARWALVPAMAELVGHAEPVLVLPAESRVLAP
                     LDDLTDHVARIGLVPRAHDAGAAIASDGWVPDLAVIGPDSAAAMAWWADVAAAAYRCA
                     PTASAIDLDDPWRTFSTAGDGITLIPDASVRLSPWSVAHLRLAIVDGAITVDDRPVRL
                     AQFPAFDVERPWWYSTSQADDPAVLTSGSAPLRRLCLEHAADLAAAIGTGDTTSPKRS
                     LVDILGVRHSDALREEVRVRTVAAMDAGELPPNPFDPESAEQFFSWLRDPADAELTGV
                     SVAADLVWRERPDLAAAFPHVRWGDRDNFLRWLWIHGLKEGLISTALLPDLPGTATPA
                     TVISPSGTDELKFGVNLVGYHDSDLGLGVAVRRVAAALDAAAIPWTKVTYDRTHSRRR
                     REGGGPASAAAPYRYNLILIAPDQLAFFVDDVGTAFLDGRYNIGLWYWETDVLSDRQV
                     AALGLIDEIWGSTQYLVDIFAAHTTRPVVRIPVPLEFRQAPPTPGRREALGLDERFTF
                     LFTFDFFSIVERKNPLELVEAYTAAFSPEDGCRLILKSINGARHLSESEELRAAFGDR
                     DDIELWDRYL"
     gene            1979..2296
                     /locus_tag="PKY13_10870"
     CDS             1979..2296
                     /locus_tag="PKY13_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80481.1"
                     /translation="MALSPSPRAIARRILGRSQLETVEQLARQQEILQRSDRERLDAI
                     EERVAALTLTLDGVSASVDRLHATIVAADPQMMRSVLESVRADVTQLSADVSSQLARI
                     AQG"
     gene            complement(2287..3903)
                     /locus_tag="PKY13_10875"
     CDS             complement(2287..3903)
                     /locus_tag="PKY13_10875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT80482.1"
                     /translation="MPIEYPEPLISILTPVYNTDIDVLELCVQSVLDQTYGHWELCLV
                     DDASPAPHVWPLLQEIATRDPRILIGRREVNGGIVAASNDCAALANGDVVSLLDHDDA
                     LHPSALRKVADAFVHIENVDYVYSDEDVVDPQGRRVAPFYKPDWSPERFRCQMYTCHL
                     SSIRRELFDRVGGFRDGFDGSQDWDLVLRVTEQARRIVHLPEILYHWRTVPTSVLAGD
                     GVKPYAYEAAKRAIAAHCERVGIDGEVEELERRGHFRVRRSVDEQQLISIVIPTRGGA
                     GRPWGQRRVMVVEAVRSVVERSTYENIEIVVVYDSVTPQSVLDELRRIADDRLRLIEW
                     TLPFDFSAKCNLGAAHAAGDLLLFLNDDVEVITADWLDVLSGFLREDDVGAAGAHLLF
                     EDGRLQHGGHVHLNGMAGHLMFGQDPHSDRNRLALWLDREVAGVTAACLMTRADVFSE
                     VGGFSSEFPNNYNDVDLSLKIRSTGRRIVVSPHARLFHFESITRDPTVVPTETRRLHE
                     RWYPELHRDPYYNPNHLGGLDSYPEPMAYP"
     gene            complement(3971..6499)
                     /locus_tag="PKY13_10880"
     CDS             complement(3971..6499)
                     /locus_tag="PKY13_10880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT80483.1"
                     /translation="MTTLPFVRVIVLNWNAAWLTTRCVRSIQRTDYPSDRFEIVVVDN
                     ASIDGSLARLRHDLDGVRFVANDHNLGFAEGNNRALRDLEGCDAVALVNNDAVVEPGW
                     LYPLVAALDDPTVGAVAPKMLLEAPFTPVRLSGAGIVERVTVDGVDVTRRCVYDGVVE
                     RAHETVPLGLVRSFDGSATVHVPVGSEPADIVVSFDDDRHLAVRSGPHGDRRINSLGT
                     ALTPWIEGFERWFGERDRPHLAPHEVWGFSGGGVLLRAAMLREVGVFDPRFFAYYEDT
                     DLAWRARRHGWRTMCSPESVVHHLHGGSAGPEAQGFFFLNYRNWLLTAARNASPRQAL
                     AAAAVARRLSWGPFRRNVFGRIRRWQRPETAISIAWARVFAGVAAALPSAIASRVTRR
                     RVGSVPTDTVRSLLMPATPPRPPRPRAGGPLLCYVDVTETLRSGWRAGIQRVVCELVK
                     ALPNARPDIELVPVCWSKVHSAFRRLDADEYASLLEPTTAQQPAGPPPPPTAARKALA
                     LAMHRSGAAPVVHSFRRRRELAAVPQHHRDLLLDRFEPGTVFFDLDATWNPTTQPRSD
                     LLPQLVASGVHVAVLQHDLIPHTHPNWFIPQMVDVFMAHVDAHLDAGSAFLCNSEHTR
                     SDLLEYAAGRGAVPTASVFRLGDTPLVSATPPEPANDAPAGAGPYFLTVGTVEPRKGH
                     ATILDAFDKVRADHPDAKLVVVGRAGWNNSGVVERLTERPQGVVWRSDVSDAELDRLY
                     RGAVVVIVASVTEGFGLPVVEALQRGVPVLSSRGGALPEAGGDLVEYFDTGSSDQLHA
                     LMERHLTDADHHAARVGIVGEFQPNAWSDAAAAVGQVLRSLGDE"
BASE COUNT         1083 a         2132 c         2450 g          989 t
ORIGIN      
        1 aggtacctgt cccacagttc gatgtcgtcg cgatccccga atgcggcccg caactcctcg
       61 gattccgaca agtgccgcgc gccgttgatc gacttgagga tgagtcgaca gccgtcctcg
      121 ggagagaacg cagcggtgta ggcctccacc agctcgagcg ggttcttgcg ctcgacgatc
      181 gagaagaagt cgaacgtgaa caggaaggtg aaacgctcgt cgagccccaa cgcctcacgg
      241 cggcccggcg tggggggtgc ctggcggaac tccaacggaa ccgggatcct gacgacgggc
      301 cgggtggtgt gggcggcgaa gatgtcgacg aggtactgcg tcgagcccca gatctcgtcg
      361 atcagcccga gcgcggcgac ttgccgatcg ctcagcacgt ctgtctccca gtaccagagc
      421 ccgatgttgt agcggccgtc gaggaatgca gtcccgacgt cgtcgacgaa gaacgccagt
      481 tggtccggtg cgatgaggat gaggttgtag cggtacggag cggctgccga ggctgggcca
      541 ccaccttcgc ggcgtcgacg gctgtgggta cggtcgtagg tgaccttcgt ccacgggatg
      601 gcggcggcgt cgagtgcggc cgcgacgcgc cggacggcga cgcccagccc gaggtcggag
      661 tcgtgatagc ccacgaggtt cacgccgaac ttcagctcat cggtcccgga aggcgagatc
      721 actgtggccg gcgtcgcggt gcccgggaga tcgggcagca gggccgtgct gatcaggcct
      781 tccttgaggc cgtggatcca caaccagcgg aggaagttgt cgcggtcgcc ccaccgtaca
      841 tgcgggaagg cggcggcgag gtcgggtcgc tctcgccaga cgaggtcggc ggcgacactc
      901 actcccgtca gctcggcgtc ggcgggatcg cggagccagg agaagaactg ctccgccgac
      961 tcggggtcga atgggttcgg cggaagctcg ccggcatcca tcgccgccac ggtccggacg
     1021 cgcacctcct cgcggagggc atcactgtga cgcacgccga ggatgtcgac cagggatcgt
     1081 ttcggcgatg tcgtgtcgcc cgtcccgatc gcggctgcga ggtcggccgc gtgttcgagg
     1141 cagagccggc gcaacggggc cgacccgctg gtgagcacgg cgggatcgtc ggcctgggac
     1201 gtggagtacc accacgggcg ttccacatcg aacgccggga actgcgccag tcgaacgggt
     1261 ctatcgtcga cggtgatcgc gccgtcgacg atcgcgaggc gcaggtgggc gacggaccac
     1321 ggcgacagtc gcacggacgc atcggggatg agggtgatgc cgtcgccggc ggtgctgaac
     1381 gtgcgccatg ggtcgtcgag gtcgattgcc gacgccgtgg gagcgcaccg gtaggccgca
     1441 gccgcgacat cggcccacca tgccatcgcg gcggcggagt cggggccgat gacggcaagg
     1501 tctggcaccc agccgtcgga ggcgatcgcg gcgccggcgt cgtgggcccg cgggacgagg
     1561 ccgatccttg cgacgtggtc ggtgagatcg tcgagtgggg cgagcaccct ggactcggcg
     1621 ggaaggacga gcaccggttc cgcgtggccg acgagctcag ccatcgccgg caccagcgcc
     1681 caccgggcga aggccgggcc accgccgagc cacgcgtcga tgtagcgaac gcccgcgacg
     1741 gtcaggtggc gcgccgatac ggtggaggtg gcccccggca ccggcgctgc gagttggagc
     1801 gggtgggtga cgaccacggt gatggtagcg gcgggatggt gctcgcggag ctgtcgtacc
     1861 acgagcacca catcggggct cgtggccgct tcggctgtca cgatcgcgtg cattcgggtc
     1921 ccaccgccgt tcggccaacc ggcgcggtcg agctcgaccg cagtagcctc cgaccctaat
     1981 ggctctttcc ccgtcccccc gcgccatcgc ccgccgcatc ctcggtcgct cccagctcga
     2041 gactgtcgag cagctggcac gacagcagga aatcctccag cggtccgatc gcgagcgcct
     2101 tgacgccatc gaggagcgtg tcgccgccct tacgttgacg ctcgatgggg tgtcggcgtc
     2161 ggtcgatcgg ctgcacgcca cgatcgtcgc cgccgacccg cagatgatgc ggtcggtgct
     2221 cgagtcggtc cgtgccgacg tcacccagct gagcgccgac gtcagctcac agctcgcccg
     2281 aatcgctcag gggtaggcca tcggctccgg ataggagtcg agcccaccga gatggttggg
     2341 attgtagtac gggtcgcggt gcagctcggg gtaccagcgt tcgtggagtc gtcgcgtctc
     2401 ggtcggtacg acggtcggat cccgggtgat cgactcgaag tggaagagcc gggcgtgcgg
     2461 cgacacgacg atgcgtcgac cggtcgagcg gatcttcaac gacaggtcga cgtcgttgta
     2521 gttgttgggg aactcggagc tgaagccgcc gacctcggag aagacgtcgg cccgagtcat
     2581 gagacacgca gcggtgacac cggcgacctc ccggtcgagc cagagcgcca ggcggttcct
     2641 gtcgctatgc gggtcctggc cgaacatgag atggccggcc atgccgttga ggtgcacgtg
     2701 gccgccgtgc tggagccgac cgtcctcgaa caggaggtgg gcgccggcgg cgccgacgtc
     2761 gtcctcacgg aggaaaccgc tgagcacgtc gagccagtcg gcggtgatga cctcgacgtc
     2821 gtcgttgagg aacagcagga ggtcaccggc ggcatgcgcc gctccgaggt tgcacttcgc
     2881 ggagaagtcg aacggcagag tccactcgat cagacggaga cggtcatcgg cgattcggcg
     2941 caactcgtcg agcaccgact ggggggtcac cgagtcgtac acgacgacga tctcgatgtt
     3001 ctcgtacgtg gaccgctcca cgacggaacg taccgcctcc accaccatca cgcgacgttg
     3061 gccccacgga cggccggccc cacctcgagt ggggatgacg atgctgatca gctgctgctc
     3121 gtcgacggac cgccggacgc ggaagtggcc tcgtcgttcg agctcctcga cctcgccgtc
     3181 gatgccgacc cgctcgcaat gagcggcgat ggcacgcttg gcagcctcgt aggcgtaggg
     3241 cttgacgccg tcgcccgcca gcactgacgt cgggacggtc cgccagtggt agaggatctc
     3301 gggcaggtgc acgattcggc gggcctgctc ggtcacgcgc agcacgagat cccagtcctg
     3361 ggaaccgtcg aatccgtcgc ggaacccgcc gacccggtca aacagctcgc gacggatcga
     3421 cgacagatgg caggtgtaca tctggcagcg gaagcgttcc ggcgaccagt cgggcttgta
     3481 gaacggcgcg acccggcgac cctgtgggtc gaccacgtct tcgtcggagt acacgtagtc
     3541 gacgttctcg atgtgcacga acgcgtcggc gaccttgcgg agggcggacg ggtgcagggc
     3601 gtcgtcgtgg tcgaggagcg acacgacgtc accgttggcg agggcggcgc agtcgttcga
     3661 cgccgcgacg atgccgccgt tgacctcacg gcggccgatg aggatgcgcg ggtcgcgggt
     3721 ggcgatctcc tggagcagcg gccacacgtg cggggccggg gaggcgtcgt cgacgaggca
     3781 cagctcccag tgcccgtagg tctggtcgag caccgactgc acacacaact cgaggacatc
     3841 gatgtcggtg ttgtagacgg gcgtgaggat cgagatcagc ggctcggggt actcgatcgg
     3901 catccgcggc atcgtatcgg ccgtcatcgg tggctcctgg cgggtgggtt gatcggattg
     3961 gtcacggtgt tcactcgtcg ccgagcgagc ggaggacctg gccgacggcg gctgcggcgt
     4021 cggaccaagc gttcggttgg aactcgccga cgatgccgac gcgagcggcg tggtgatcgg
     4081 cgtcggtgag gtggcgctcc atgagcgcgt gtagctggtc gctcgagccg gtgtcgaagt
     4141 actccacgag gtcaccgccg gcttcgggca gcgcgccgcc ccgcgacgac aggacgggca
     4201 caccacgttg cagggcctcc acgacgggca ggccgaagcc ctccgtgacc gacgccacga
     4261 tcacgacaac ggcgccgcgg tagaggcggt cgagctcggc gtcggataca tcggagcgcc
     4321 aaacgacgcc ctgaggccgc tcggtcaagc gctcgaccac cccgctgttg ttccatccgg
     4381 cgcgcccgac taccacgagc ttggcgtcgg gatgatcggc tcgcaccttg tcgaacgcat
     4441 cgaggatcgt ggcatggccc ttgcgcggct ccacagtgcc gacggtcagg aagtaggggc
     4501 ccgcccccgc cggtgcgtcg tttgcgggct ccggtggcgt cgctgagacg agtggagtgt
     4561 cgccgaggcg gaacaccgaa gcggtcggga cggcgccacg cccggcggcg tactcgagca
     4621 ggtcgctgcg ggtgtgttcg gagttgcaga ggaacgcgga gccggcgtcg aggtgcgcgt
     4681 cgacgtgtgc catgaacacg tcgaccatct gcggaatgaa ccaattcgga tgggtgtgtg
     4741 ggatcaaatc gtgttgcagc acggcgacgt ggacaccgct tgctaccagc tgcgggagga
     4801 ggtcagaccg cggttgggtc gtcgggttcc aggtggcgtc gaggtcgaag aagaccgtgc
     4861 caggctcgaa ccggtcgagc aacagatctc ggtggtgctg cggcaccgcg gcgagctcgc
     4921 ggcggcggcg gaagctgtgg acgaccggtg ctgcgccgct ccgatgcata gccagggcca
     4981 gtgccttgcg agccgcggtc ggtggcggcg gaggaccggc cggctgctgg gcggtggtcg
     5041 gctcaagcag gctcgcatac tcatcggcat ccaagcggcg gaaggcgctg tgaaccttcg
     5101 accagcacac ggggacgagc tcgatgtcgg gcctggcatt tgggagcgct tttaccagct
     5161 cgcacacgac tcgttggata ccggcccgcc atcccgaccg cagtgtctcg gttacgtcca
     5221 catagcagag gagcgggcca ccggcccggg gtcgcggggg tcgcggaggt gtcgccggca
     5281 tgaggaggct acgcaccgtg tcggtgggca ccgaaccgac ccgacggcga gtaacgcgcg
     5341 aggcgatggc cgatggcaac gccgccgcga ccccggcgaa cacgcgggcc cacgcgatgc
     5401 tgatcgccgt ctcggggcgc tgccatcggc gtatgcgacc aaagacgttg cgccggaacg
     5461 ggccccagga caggcgccgc gccaccgccg ccgccgccag ggcctgtcga ggcgaggcgt
     5521 tgcgtgccgc ggtcaggagc caattgcggt agttcaggaa gaagaacccc tgcgcctcgg
     5581 gcccggcgct gcccccgtgt aggtggtgga cgaccgactc gggcgagcac atcgtgcgcc
     5641 agccgtggcg ccgggcccgc caggctaggt cggtgtcctc gtagtatgcg aagaagcggg
     5701 ggtcgaacac gccgacctcg cgcagcatcg ccgcccgcag gaggacgccg ccgccgctga
     5761 agccccagac ctcgtgtggg gcgaggtggg ggcgatcccg ctcgccgaac caacgttcga
     5821 acccctcgat ccagggcgtg agcgcggtcc cgaggctgtt gatgcggcga tcaccatgcg
     5881 gacccgagcg gaccgcgagg tggcgatcgt cgtcgaacga gaccacgatg tcggcgggct
     5941 cgctcccgac cgggacgtgc accgtggcgc tgccgtcgaa cgaccggacc agaccgaggg
     6001 gcacggtctc gtgcgcccgc tcgaccacgc cgtcgtagac gcaacggcgg gtgacgtcga
     6061 cgccgtccac cgtgacccgt tccacgatgc cggcgccgct caggcggacc ggggtgaaag
     6121 gcgcttcgag caacatcttg ggagcgacgg cgccgaccgt cgggtcgtcc agcgccgcca
     6181 cgaggggata cagccaaccg ggctcgacga cggcgtcatt gttgacgagt gccacagcgt
     6241 cgcacccctc taggtcgcga agggcgcggt tgttcccttc ggcgaagccg aggttgtgat
     6301 cgttggcgac gaagcgaacg ccgtcgaggt cgtggcgcag ccgagcgagg gagccgtcga
     6361 tcgacgcgtt gtcgaccacc acgatctcga accggtcgct ggggtagtca gtgcgttgga
     6421 tggaccggac gcatcgggtg gtcagccacg cagcgttcca gtttaggacg atgacgcgaa
     6481 cgaacggcag ggtcgtcatt gcgccgatca gcgcaccagg cgacgggctg ccttgagtgg
     6541 cctgagcaca ccgtcgccca ggcggaatgt gagtgactgc tcgacgtggg ccaggcgggc
     6601 gatctcggat cgcagctggt gaaccatcgc ctcgagctcg gcgttgcgcg cccg
//
LOCUS       DAOSOA010000171         6645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11618_length_6645_cov_5.809256, whole genome shotgun sequence.
ACCESSION   DAOSOA010000171 DAOSOA010000000
VERSION     DAOSOA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11618_length_6645_cov_5.809256"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..697)
                     /locus_tag="PKY13_10885"
     CDS             complement(116..697)
                     /locus_tag="PKY13_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80484.1"
                     /translation="MNQLTRLIAATVLTATLAGPVVGLDATTASAQTTDSRKAPCKPY
                     PWHLHKHTYSNIRVVSRQTQNLGQVSQAIRNPYSSSIRTQVRVDRAKSKQISVNASVE
                     VEASGLVAAVRASTGIDVATSVTSTVGVDVPLIIPAKKFGWVQAENETVTITATVTSL
                     DLMCNVVDYRGTIRSTMPRSKAYLTPKVAASAP"
     gene            complement(788..2845)
                     /locus_tag="PKY13_10890"
     CDS             complement(788..2845)
                     /locus_tag="PKY13_10890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOT80485.1"
                     /translation="MSLATRSLDLLDRVRESMDAGEDRPGQHQMVEAIATAIGEKQHL
                     VVQAGTGTGKSLAYLIPALTSGRRTIVATATKALQDQLATKELPLLAKHMSKKISWAV
                     LKGRSNYLCLQRLDEATKPEAQLGLDGVAEAAGGTSRIPDKTLKELQKFAETSSTGDR
                     ADLPTEPQPWVWSAVSVGPRECPGATKCPRGEDCFAERARSLAAAADVVIVNTHLYGL
                     NLGSRGAVLPDHDVVIFDEAHEVEDIISATTGLELGPGTITSIARTISGLIADDSVTD
                     GLEDDATRLTRQLAPLVGSRLRRGLPPETADLLNATRARVMEASAALKKIPDSGSGDT
                     ATRKARVATAVGGLVLDIDALVSPPGDTVMWVGGTEDHPRIEMAPLDVGATLDSLLWD
                     PEPNGLEIVIGGGSSAGPTGGPTIDLVDDEPFGTGRSRGGDETDPTFGLPDTVVFTSA
                     TVPTALVERLHIPPDEVTVIDVGTPFDFANHALLYCATHMPDPRSAKYGEAVADELVR
                     LITAAGGRTLALFTSHRAMNEAADAVAARVDLPVLRQGDRPKPALIEEFADDEASCLF
                     ATMGFWAGIDVPGPSLSLVTIDKIPFPRPDEPLLAARREKAAAQGFATIDLPRAATLL
                     AQGAGRLIRSATDQGVVAVLDPRLSTKPSYRWQLIHALPPMKRTKDFDEVAAFLAALG
                     TPV"
     gene            2918..3640
                     /gene="uppS"
                     /locus_tag="PKY13_10895"
     CDS             2918..3640
                     /gene="uppS"
                     /locus_tag="PKY13_10895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOT80486.1"
                     /translation="MELSDLDPTYIPRHVAVVMDGNGRWAQQRGLPRTDGHAAGEEAL
                     MDTIWGAHAAGIRWLTVYAFSTENWKRPLDEVRFLMNFNERLLLTRRDEIHARNIKMQ
                     FLGRRDWRVPRRILKRIDETLELTKDNDAMTVSMAFNYGGRAELVDAVRKIVEAGVPA
                     NKINEKMISAHMYDPTMPDPELVVRTSGEIRTSNYLMWESAYSEWVFTDVLWPDFRRE
                     NLYDSIREYQQRERRFGGLANE"
     gene            3643..4362
                     /gene="recO"
                     /locus_tag="PKY13_10900"
     CDS             3643..4362
                     /gene="recO"
                     /locus_tag="PKY13_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOT80487.1"
                     /translation="MPLYRDVGVVLRTYKLGEADRIVVFCTQGRGKVRAVAKGVRKTK
                     SRFGSRLEPGSHVSLQLYEGRELDIVSQAELVEAQQALRDDLDRMGRAAAMLEAVDQI
                     CQEGEVNPALYDMLVGGLRAVANWDTPLVTPSFFFKLLAAEGVGLQVDCCLECGAEDD
                     LVALDLDGGGLRCRTHRDGLAVSSQAVTILQLVLGGRLSNALALPIGPATFEVDHLAS
                     VAMERHLDRRLRSLHLLDHPG"
     gene            complement(4451..5458)
                     /locus_tag="PKY13_10905"
     CDS             complement(4451..5458)
                     /locus_tag="PKY13_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80488.1"
                     /translation="MSDPNDPYSGGATPPPPPFGGGAITPPPGPGATPPPPAPPGGGY
                     QAPPPPAPPGGGYTPPPPPGPPGGGYAPPPPGGGYVPPPAPPGGYQAYQPGGFPSGGG
                     QADIGTAFSWTFAKFQQHLVPLLSLSGVIFLVNLLGGITASKLAETSVDDLRINDNGV
                     LVNDGNFWSGIIGSILISIVIALLVAFLRIGLLRAALRVTRGETPSFNDLMTGTNLVP
                     YIVVAIVSGILTAIGLVLCVIPGLLVAFFLLFAPIHALDKGAGVGDALNWSFNAVKAN
                     VVPCIVLVLIGIVVGIISSFAGGIGGAVVAALLGLFIDPISSLLTANLYRQMGNEPIA
                     A"
     gene            complement(5572..5967)
                     /locus_tag="PKY13_10910"
     CDS             complement(5572..5967)
                     /locus_tag="PKY13_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOT80489.1"
                     /translation="MAQHSIDPENLPYEPSKLPRKHDISGPDFFAVDMRVGEVVDVRR
                     FPEARKPAYQLVVDFGPVVGILETSAQVTNYDRDELVGRRVVGAINLGVRRIAGFESQ
                     FLVLGGLAPDGTVHLLAPDADVPLGAPVA"
     gene            6033..>6645
                     /locus_tag="PKY13_10915"
     CDS             6033..>6645
                     /locus_tag="PKY13_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOT80490.1"
                     /translation="MSEAESAVAPDPEIFDKIVNLSKRRGIAFQSAEIYGGFRSTYDY
                     GPVGVLLLRNVKDAWWRSMVQMRHDVVGLDAAILGPPAIWEASGHLANFTDPLVDCTV
                     CQSRHRLDKLEDPNVCPTCGSKGSFTEPRDFNLMFKTQAGPVAGAGADCYLRPETAQG
                     MFVNFANVLNSTRKKPPFGIAQIGKSFRNEITPGNFVFRTREFE"
BASE COUNT         1035 a         2119 c         2437 g         1054 t
ORIGIN      
        1 gtcaccttgc cacggagcgt cagtcatgag aacgatcttc tcactccgcg gccgagccgc
       61 ccaaacgggc ggctcggcgg gacctgggcg atgtggatgg gcgggtcggc gccggtcagg
      121 gcgccgaagc ggccaccttg ggtgtcaggt aggccttgct acggggcatg gtcgaacgga
      181 tcgtgccccg gtagtcgacc acattgcaca tgaggtcgag cgaggtcacc gttgcggtga
      241 tcgtgaccgt ctcgttctcg gcctggaccc agccgaactt cttcgccggg atgatgagcg
      301 gcacatccac accgacggtc gacgtgaccg atgtggcgac atcgatcccc gtggaggctc
      361 gcacagcggc cacgagcccg ctggcctcga cctcgacgct ggcgttgacg gagatctgct
      421 tcgacttcgc ccggtcgacc cgcacctggg tccggatcga ggagctgtac ggattgcgga
      481 tcgcctggct gacctggccg aggttctggg tctggcggct gacgacacgg atgttggagt
      541 acgtgtgctt gtggagatgc cacgggtagg gcttgcacgg cgccttgcgg ctgtcggtgg
      601 tctgggccga cgccgtggtt gcgtcgagac cgacgaccgg gccggcgagg gtggcggtga
      661 ggacggtggc ggcgatgagg cgggtgagct ggttcatggg acgactcctt gggtgaggtg
      721 tcgacggtgg ttccgttcga caacctcatc ttccagtggg tcccttcagt gctccttcaa
      781 gggtgcttca gacgggagtg cccagcgcgg cgaggaaggc ggcgacctcg tcgaagtcct
      841 tcgtccgctt catcggtggc agggcgtgga tgagttgcca ccgatagctc ggcttcgtcg
      901 acaggcgtgg gtcgagcacc gcgacgacgc cttgatcggt cgccgagcgg atgaggcgac
      961 ccgcaccctg ggccaacagc gtcgcggcac ggggcaggtc gatggtggcg aacccctgag
     1021 ccgcggcctt ctcgcgtcgc gctgccagga ggggttcgtc ggggcgcggg aacgggatct
     1081 tgtcgatcgt gaccaggctc agcgacgggc cgggcacgtc gatgccggcc cagaacccca
     1141 tcgtcgcgaa gaggcatgac gcttcgtcgt cggcgaactc ctcgatgagc gcgggcttgg
     1201 gccggtcgcc ctggcggagg acgggcaggt cgacccgcgc cgccacggcg tcggctgctt
     1261 cgttcatggc ccggtggctg gtgaagaggg ccagggtgcg gccgccggcc gcggtgatga
     1321 ggcggacgag ttcgtcggcc acggcctcgc cgtacttggc cgaccggggg tcgggcatgt
     1381 gggtcgcgca gtagaggagc gcgtggttcg cgaagtcgaa cggggtgccc acgtcgatga
     1441 cggtgacctc gtcgggcggg atgtggaggc gttcgacgag cgcggtgggg acggtggcgc
     1501 tcgtgaacac gaccgtgtcg ggcagcccga acgtcggatc ggtctcgtcg ccgccccgtg
     1561 accgcccggt gccgaacggc tcgtcgtcca cgaggtcgat ggtcggtcct ccggtcgggc
     1621 cggccgacga tccgccgccg atgacgatct cgaggccgtt cggttcggga tcccacagca
     1681 gggagtcgag cgtcgcaccc acgtcgaggg gagccatctc gatgcgcggg tggtcctcgg
     1741 tgccgcccac ccacatgacg gtgtcgccgg gtgggctgac cagcgcgtcg atgtcgagca
     1801 cgaggccccc cacggccgtg gccacccggg ccttgcgggt cgccgtgtcg cccgatccgc
     1861 tgtcggggat cttcttgagt gctgcggacg cctccatgac ccgcgcccgc gtggcgttga
     1921 ggaggtcggc ggtctcgggc gggaggccac gccggagccg agagccgacg aggggagcga
     1981 gttggcgagt gagccgcgtc gcgtcgtcct cgagtccgtc ggtgacggag tcgtcggcga
     2041 tcaggcccga gatcgtgcgg gcgatcgatg tgatcgttcc ggggccgagc tccaggccgg
     2101 tcgttgccga gatgatgtcc tcgacctcgt gcgcctcgtc gaagatcacc acgtcgtgat
     2161 cgggaaggac cgccccgcgc gacccgaggt tcagcccgta caggtgggtg ttgacgatga
     2221 cgacgtcggc ggcagcggcc aacgagcggg cccgctcggc gaagcagtcc tcgccgcgtg
     2281 ggcacttcgt tgcacccgga cactcgcggg ggccgaccga gaccgccgac cagacccacg
     2341 gctggggctc ggtcgggagg tcggcgcggt cacccgtcga cgacgtctcg gcgaacttct
     2401 ggagttcctt cagcgtcttg tcggggatgc ggctggtgcc gccggcggct tcggcaacgc
     2461 cgtccaggcc cagctgcgcc tcgggtttgg tcgcctcgtc gagtcgttgg aggcacaggt
     2521 agttcgagcg acccttgagc acggcccacg agatcttctt gctcatgtgc ttggccagga
     2581 gcgggagctc cttggtggcc agttggtcct ggagcgcctt ggtggcggtc gcgacgatgg
     2641 tgcgccggcc cgaggtcagc gccgggatga ggtaggcgag cgacttgccg gtgcccgtgc
     2701 ccgcctggac gaccaggtgc tgcttctcgc cgatcgccgt ggcgatcgcc tccaccatct
     2761 ggtgctggcc gggtcggtcc tcgccggcgt ccatcgactc gcggacgcgg tcgaggaggt
     2821 cgagtgatcg ggtcgccaaa ctcacgtccc tactctcgca tgtgaaggaa cggtggctgg
     2881 gcacgggggt ccacacgcca gcaggtacgg tgcgggtgtg gagctgagcg acctggaccc
     2941 gacctatatc cctcgccatg tggcggtcgt catggatggc aacgggcgat gggcccagca
     3001 gcgcggcttg ccccgcaccg acggccatgc cgccggtgag gaggcgctca tggacacgat
     3061 ctggggagcg cacgccgccg gcatccgctg gctcacggtg tacgcgttct cgacggagaa
     3121 ctggaagcgt ccgctcgacg aggtccggtt cctcatgaac ttcaacgagc ggctgttgct
     3181 cacccgccgc gacgagatcc atgcccgcaa catcaagatg cagttcctcg gccggcgcga
     3241 ctggcgagtg ccgcgccgca tcctcaagcg catcgacgag accctcgaac tcacgaagga
     3301 caacgacgcc atgacggtgt cgatggcctt caactacggc ggtcgcgccg agctggtcga
     3361 cgccgtgcgc aagatcgtcg aagcgggtgt gccggccaac aagatcaacg agaagatgat
     3421 cagcgcccac atgtacgacc cgacgatgcc cgatcccgag ctcgtggtcc gcacctcggg
     3481 cgagatccgc acgtcgaact acctgatgtg ggagtcggcg tactcggagt gggtgttcac
     3541 cgacgtcctg tggccggact tccggcgcga gaacctctac gactccatcc gcgagtacca
     3601 gcaacgggag cgccgcttcg gcggtctcgc caacgagtag ccgtgccgct ctaccgcgac
     3661 gtcggcgtgg tgctccgcac ctacaagctc ggcgaggccg atcgcatcgt cgtgttctgc
     3721 acccagggtc gcggcaaggt gcgcgctgtc gcgaaaggcg tgcgcaagac gaagtcacgg
     3781 ttcgggtcgc ggctcgagcc cggcagccac gtgtcgctgc agctctacga gggccgcgag
     3841 ctcgacatcg tgagccaggc cgagctggtc gaggcgcagc aggcgttgcg tgacgacctc
     3901 gaccgcatgg gtcgcgccgc ggcgatgctc gaagccgtcg accagatctg ccaggagggc
     3961 gaggtcaacc ctgcgctcta cgacatgttg gtgggcgggc tgcgggccgt cgcgaactgg
     4021 gacaccccgc tcgtaacccc gtccttcttc ttcaagctgc tcgcagccga aggcgtcggc
     4081 ctgcaggtcg actgctgcct cgagtgcggg gcagaagacg atctcgtcgc cctcgacctc
     4141 gatgggggtg ggttgcggtg ccgcacccac cgcgacggac tcgccgtgtc gagccaggcc
     4201 gtgaccatcc tccagctggt gttgggtgga cggctgtcga acgcgctcgc gctcccgatc
     4261 ggtccggcga ccttcgaggt cgatcacctg gcgtcggtcg ccatggagcg gcacctcgac
     4321 cgtcgcttgc gctcgttgca cctgctcgac catccgggct gaccccgaaa caagcccgaa
     4381 ccagaccccg aacgcggaac gaccccgggc cggagcccgg ggtcgttcgg tgatcggggt
     4441 gatcgctcga tcaggcggcg atcggctcgt tgcccatctg gcggtagagg ttggccgtca
     4501 ggagggacga gatcgggtcg atgaacagcc cgagcagtgc ggcgacgaca gcgccaccaa
     4561 tgccaccggc gaacgagctg atgatgccaa cgacgatgcc gatcagcacg agcacgatgc
     4621 agggcaccac gttcgccttg accgcgttga agctccagtt gagcgcatcg ccgacgccgg
     4681 cccccttgtc gagggcgtgg atcggtgcga acagcaggaa gaacgcgacc aggaggccgg
     4741 ggatgacaca cagcacgagg ccgatcgccg tcaggatgcc ggacacgatc gccacgacga
     4801 tgtagggcac gaggttcgtg ccggtcatca ggtcgttgaa cgacggggtc tcgccgcggg
     4861 tgacccggag tgcggcgcgg agcaggccga tgcggaggaa cgcgacgagc agggcgatca
     4921 cgatcgagat caggatgctg ccgatgatgc cgctccagaa gttgccgtcg ttgacgagca
     4981 caccgttgtc attgatgcgg aggtcgtcga cggaggtctc cgccagcttc gatgcggtga
     5041 tgccgcccag caggttgacg aggaagatca cgccggagag cgagagcagc ggcacgaggt
     5101 gctgctggaa cttcgcgaag gtccagctga acgcggtgcc gatgtcggcc tggccgccgc
     5161 cgctcgggaa gccgccgggc tggtacgcct ggtagccgcc aggaggcgcg ggcgggggca
     5221 cgtagccgcc accgggaggc ggcggggcgt aaccaccgcc gggcggacca ggaggcggag
     5281 gaggggtgta accgccgccg gggggcgctg ggggaggagg cgcttggtag ccgccaccgg
     5341 gaggggccgg agggggcggg gtcgccccgg gaccgggcgg cggggtgatg gcaccgccac
     5401 cgaacggcgg cggcggcgga gtggcgcctc cggagtacgg gtcgttggga tcgctcacga
     5461 gcagctcctt gggcagtgct tgaggaagta gacggtcacg ctatcaacgc ggctgcgcga
     5521 aaccggggaa catcacacga acgaaacggt cgtcgccggt gcgtaggtgc gtcaggcgac
     5581 cggcgcgccg aggggcacgt cggcgtcggg tgcgaggagg tggacggtgc cgtcgggggc
     5641 gagtccgccg aggacgagga actgcgactc gaagccggcg atgcgccgaa ccccgaggtt
     5701 gatggcgccc accacacgcc ggccgaccag ttcgtcgcgg tcgtagttgg tcacctgggc
     5761 gctcgtctcg aggatgccga cgaccggtcc gaagtcgacg acgagctggt acgccggttt
     5821 ccgggcctcg gggaagcggc ggacgtcgac cacttcaccg acccgcatgt cgactgcgaa
     5881 gaagtcgggg ccactgatgt cgtgtttgcg ggggagcttc gacggttcgt acggcaggtt
     5941 ctcggggtcg atcgaatgtt gggccatgtc gcgaacctac ggcggggaac caggtggggg
     6001 agcacatctc ccacccggta acctcggcct ctatgtccga agcagagtcc gccgtcgccc
     6061 ccgatcccga gatcttcgac aagatcgtca acctgtcgaa gcgtcgcggt atcgccttcc
     6121 agtcagcgga gatctacggc gggttccgct cgacctacga ctacgggccc gtcggtgtgc
     6181 tgctgctccg caacgtcaag gacgcctggt ggcgctcgat ggtgcagatg cgccacgacg
     6241 tcgtgggcct cgacgccgcg atcctcggtc cgccggcgat ctgggaggcc tcgggtcacc
     6301 tcgccaactt caccgatccg ctggtcgact gcacggtgtg ccagtcgcgt caccgtctcg
     6361 acaagctcga ggatccgaac gtgtgcccca cgtgtgggtc gaagggcagc ttcaccgagc
     6421 cgcgtgactt caacctgatg ttcaagacgc aggccggccc ggtcgccggc gccggtgccg
     6481 actgctacct ccgccccgag acggcgcagg gcatgttcgt gaacttcgcc aacgtgctca
     6541 acagcacgcg caagaagccg ccgttcggca tcgcccagat cggcaagtcg ttccgcaacg
     6601 agatcacgcc cggcaacttc gtgttccgca cccgcgagtt cgagc
//
LOCUS       DAOSOA010000172         6633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11648_length_6633_cov_5.992399, whole genome shotgun sequence.
ACCESSION   DAOSOA010000172 DAOSOA010000000
VERSION     DAOSOA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11648_length_6633_cov_5.992399"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="PKY13_10920"
     CDS             complement(<1..1381)
                     /locus_tag="PKY13_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT80491.1"
                     /translation="MTGRSRRRGRSVAIVAAVLLWAVFYGWPVVAIVRRSLEGVAGQP
                     GLSFSTVGDVLSDGRVGRLAMWSVSQCLASTLVVTVLGVPMGWVLGRLRFRGRRLLSA
                     VVMTPFVLPTLTIAGAMIALAGGLPSSTAARFGLIVAAHVTFNVGLMVRSVSAAVAAV
                     PPSIEQAAATLGRSQRRAITSTTLAAIRPAIASAAIVVAVFCLTSFGVIVALGGASIG
                     TVEVEVWYLTTRSLDLGRAAVLAGGQLLVVMLLVVAYQRIHRPVRGRVAHELRAVRTN
                     VERRLVRGSVFVASLVSAAPLVALVVRSLRGPDGWTIDNYRILTGDGAGALGYSLAGA
                     IVATIIALGLAAPLVAAAARVDRVGRWFEGAMVVPLALSAATLGLGFLVAFSGPTLDL
                     RGTWIIVPLVQAASAAPVVVRIVLTAARSVDGSLLDAASVSGAGPLRRFTSVVWPALR
                     RPVGVASGFA"
     gene            complement(1476..2561)
                     /locus_tag="PKY13_10925"
     CDS             complement(1476..2561)
                     /locus_tag="PKY13_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT80492.1"
                     /translation="MSRSLPSIRPRRRFPALAAALALVAAAACGDESSSSDAGDGPPT
                     ITIVTYSSYVLDEAVQAKVEKELGVKLEVNATGDGAEALSAAILTAGRPEGDVFFGVD
                     NTLLSRALDGDVFAETASEDLPNLAAIPDELRLDDTGRLVPIDVGPVCVDYDQQWFTE
                     HQLEPPTSLQDLTKPEYRDLLVVESPVTSSTGLVFLMGTHSALGDGTSQFWKDLVANG
                     VSVAGSWDDAWGAQYTVSGGDRPLVVSYASSPPAEVVYSEGKVTTPRTGVVEATCADQ
                     IEFAGVLRGTEHPTEAAQVLNAMLGEEWQASLPLANFVYPARPGIEEPDVFAQFAPRP
                     TKTIALDPKMIGERRDEWVDEWRQIAE"
     gene            complement(2558..3232)
                     /locus_tag="PKY13_10930"
     CDS             complement(2558..3232)
                     /locus_tag="PKY13_10930"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOT80493.1"
                     /translation="MRIGVVLAGGASSQSSAEQLLPRVLADDVAMADAVIVAADSGLD
                     LARRLGIVPTLVVGDLDSVTDDALRWARGLGIEVRSFPPDKDETDLDLALAAVVVAGV
                     DRVIVLGGAGGRLDHLLGNIGAIGRLGGELPVEAWLGEEYFAVVTSSWSAALPAAAIV
                     SVLPLGGPVVVSEAGVRWPLDRHRLPLGSTQGISNEAVATDGPVRITVHEGCALVVVP
                     TAKDLT"
     regulatory      complement(3224..3333)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3494..3742
                     /locus_tag="PKY13_10935"
     CDS             3494..3742
                     /locus_tag="PKY13_10935"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT80494.1"
                     /translation="MTNNGSHRRHLHLPHLSNRHRDGDGQSVSPGWYDDPTGRFEQRY
                     WNGEEWTTEVLRLSLKHYDLDEIDLRILVSPPVSGDAG"
     gene            complement(3765..4916)
                     /locus_tag="PKY13_10940"
     CDS             complement(3765..4916)
                     /locus_tag="PKY13_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80495.1"
                     /translation="MSLFTEEHHMFRKTLRDFVEREINPHAEAWEEAGTFPAHELFPK
                     LGELGVLGLTYDPAYGGGGSDYGYVAIMAEEFGRMPCLGVAMAIGVQTDMATPSLARF
                     GTHELKEQFLAPAISGQHVAAVAVTEPDAGSDVAGIRTKATRDGDEWVINGAKTYITS
                     GTQADWVCLLVRTSDEGGSRGVSQVIVPTDTPGFIVSRKLDKLGMRSSDTAELSFDNM
                     RVPVANTIGEIGRGFQQQMSQFQDERMIASYQMVGAMEYALERTADYLKQRMAFGKPL
                     LANQHLAYELADLASEVDLLKYYNYNAAVAQTNGEDITRFATVAKLRSARLARKVADI
                     CLQFHGGIGYMEETWTARFFRDTRLWSIGGGADEVMLRTLARVDGYWVD"
     gene            4965..6269
                     /locus_tag="PKY13_10945"
     CDS             4965..6269
                     /locus_tag="PKY13_10945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT80496.1"
                     /translation="MSYDAPTPPPPGDPGRNRTPWLVALAAVVVTIVIAVAVAFMGGS
                     GDTGKITISAGGSATTSPDGDRPITGEVDVDGDALPPYLDGESDDAVGVTIPTITGTD
                     YEGRPVTIEPGTPLLIVVMAHWCPHCQAEIPRIVGWNDDGRVPEELDVIGVSTAVDET
                     RGNYPPSAWLERENWPFPVLADDDRTTALQALGSSSFPTLIAVGADGRVALRASGEGD
                     EDRFDELVAAALEGAPGDAEAPDTTTGGAPAGSAACPAADGSSAKQQEFDGPPPMCID
                     SAATYTAAVDTTMGSFTITFDPTIAPVTVNNFVFLARYHYFDGIIFHRIIDGFVIQGG
                     DPEGTGMGGPGYEFEDELPRQGSYEKYSVAMANAGPDTNGSQFFVITGPDGEALPPSY
                     SLFGKVTAGTEVIDAISAVPTDVQDAPTTAVVINSVVITEGS"
BASE COUNT         1101 a         2465 c         2182 g          885 t
ORIGIN      
        1 acgcgaagcc cgatgccacg ccgaccggtc gtcgcagcgc gggccacacg accgacgtga
       61 accggcgaag cggtccggcg cccgagacgg aggcagcgtc cagcaacgag ccgtccacgg
      121 accgagccgc cgtcagcacg atccgcacga cgacgggcgc cgccgacgcc gcttgcacca
      181 gcggcacgat gatccacgtg ccccgcaggt cgagggtcgg cccgctgaac gccacgagga
      241 acccgaggcc gagcgtcgcc gccgacagcg ccagcggcac gaccatcgcc ccctcgaacc
      301 aacgaccgac ccgatcgaca cgagcggcgg cagccaccag cggagcggcc agccccagcg
      361 cgatgatcgt ggcgacgatc gcccccgcca acgagtagcc gagcgcaccg gcaccgtcgc
      421 cggtgaggat gcggtagttg tcgatcgtcc atccgtcggg gccccgcagg gaccgaacca
      481 cgagggcgac gagcggcgcg gcgctgacga gcgaagcgac gaacacactc ccccgcacga
      541 gccgacgctc gacgttcgtc cgcaccgccc gcagctcgtg ggcgacccgg ccgcgcaccg
      601 gacgatggat ccgctgatac gcgacgacga gcagcatcac gacgaggagc tgtccgccgg
      661 cgaggaccgc ggcacgcccg aggtcgagcg agcgcgtggt gaggtaccag acctccacct
      721 cgaccgtgcc gatcgacgct cccccgagcg ccacgatcac cccgaacgac gtgaggcaga
      781 acacggccac gacgatggcg gcgctggcga tggccggccg gatggcggcg agcgtggtgg
      841 aggtgatcgc ccgccgctgc gagcggccca gggtcgccgc cgcctgctcg atcgacggcg
      901 gcacggccgc gacagcggcg ctcaccgacc gcaccatcag ccccacgttg aacgtgacgt
      961 gcgctgcgac gatcagcccg aaccgtgccg cggtggacga gggcagccct ccggcgagcg
     1021 cgatcatggc gccggcgatc gtgagggtcg gcaacacgaa cggcgtcatg acgacggccg
     1081 acagaagccg tcgcccccgg aaccgcaatc gcccgagcac ccaccccatc ggcaccccca
     1141 gcaccgtgac gaccagcgtc gacgccaggc actgcgacac gctccacatg gccagtcgcc
     1201 cgacccgtcc gtcactcagc acgtcaccga ccgtggagaa cgacagcccc ggttggccgg
     1261 ccacgccctc gagactccgc cgcacgatgg ccacgaccgg ccagccgtag aacacggccc
     1321 agagcagaac cgccgcaacg atcgcgaccg accgccctcg ccggcgcgac cggccggtca
     1381 cgagccgatg cgagttcgta atccatgcga gaagtgacgc gacacggcgc atctcgcatg
     1441 gatttggccg ccggggcggg gagctggcgc tgctctcact cggcgatctg ccgccactcg
     1501 tccacccact cgtcacgacg ctcgccgatc atcttcgggt cgagggcgat ggtcttggtc
     1561 gggcggggtg cgaactgcgc gaacacgtcg ggctcctcga tgccgggccg ggccgggtac
     1621 acgaagttcg ccaacggcaa cgacgcctgc cactcctcgc ccagcatcgc gttcaacacc
     1681 tgggcggcct cggtcgggtg ctcggtgcca cgcaggaccc cggcgaactc gatctggtcg
     1741 gcgcacgtcg cctcgaccac acccgtccgc ggcgtcgtca ccttgccctc ggagtacacg
     1801 acctcggcag gcgggctcga cgcgtaggag acaacgagcg gacggtcgcc accgctcacc
     1861 gtgtactgcg caccccaggc gtcgtcccag ctgccggcca ccgacacacc gttggcgacg
     1921 aggtccttcc agaactgcga cgtcccgtcg cccagggcgc tgtgggtccc catgaggaac
     1981 acgaggccgg tgctcgacgt gacggggctc tcgacgacca gaaggtcgcg gtactcgggc
     2041 ttcgtgaggt cctgcagcga cgtcggcggc tcgagctgat gctcggtgaa ccactgctgg
     2101 tcgtagtcga cgcacacggg gccgacatcg atcggcacca ggcgacccgt gtcatccagg
     2161 cgcagctcgt cggggatcgc ggcgagattc ggcaggtcct cgctcgccgt ctcggcgaac
     2221 acgtcgccgt cgagcgcccg cgacagcagg gtgttgtcga cgccgaagaa gacatcgccc
     2281 tcgggccgac cggccgtgag gatcgctgca gagagcgctt cggcgccgtc gccggtggcg
     2341 ttgacctcga gcttcacgcc gagctccttc tcgaccttcg cctgcaccgc ctcgtcgagc
     2401 acgtacgacg agtacgtcac gatcgtgatc gtcggcgggc cgtcgccggc atccgaggac
     2461 gacgactcgt cgccgcaggc ggccgctgcc acgagagcga gcgccgcagc gagcgccggg
     2521 aagcgacgac gcggacggat ggagggaagg gaacggctca tgtcaggtcc tttgcggtcg
     2581 ggacgaccac gagcgcgcag ccctcgtgga cggtgatgcg gacggggccg tcggtggcga
     2641 cggcctcgtt gctgatgccc tgggtggagc cgagcggaag ccggtgacgg tcgagcggcc
     2701 atcgcacgcc cgcttccgac accaccaccg ggccgccgag cggcagcacg gagacgatgg
     2761 cggcggcagg cagcgcagcc gaccacgacg acgtgacgac ggcgaagtac tcctcgccga
     2821 gccacgcctc gacgggaagc tccccgccga gccgaccgat cgcgccgatg ttgccgagca
     2881 gatggtcgag ccgaccacct gccccaccca gcacgatcac ccggtcgaca ccggccacaa
     2941 ccaccgccgc gagggcgagg tcgagatcgg tctcgtcctt gtcgggaggg aacgagcgaa
     3001 cctcgatgcc gagcccacgg gcccaccgca acgcgtcgtc ggtgaccgag tcgaggtcac
     3061 cgacgacgag cgtgggcacg atccccagac gacgcgccag gtcgagaccg ctgtcggcgg
     3121 ccacgatcac ggcgtcggcc atcgccacat cgtccgcgag gacgcgtggc agcaactgct
     3181 cggccgacga ctggctggat gcgccgcccg cgaggacgac tccgatccgc atgactcgat
     3241 tcctccgctg gcattaccca gatcaggtgt gcgggtcggt gacgcatcgg tcaccctctc
     3301 agcctggctg cccaagctcc ccgttcgttc aactcacgag tggtctagca cagcggcccg
     3361 gcgatcccga cccaccctcg acgcatttca gccaattccg gcgaggatgg cgggccgatt
     3421 tccggcagac aggggtgctc atttccggcg aagtgcttgc accacccgac cgcatttggg
     3481 aatttattga cccatgacca acaacggctc gcatcgacgg cacctgcacc ttccccacct
     3541 gtcgaaccgc caccgtgacg gcgacggcca gtcggtcagc cccggttggt acgacgaccc
     3601 gaccgggcgt ttcgagcagc gctactggaa cggcgaggaa tggaccaccg aggtgctccg
     3661 cctcagtctc aagcactacg acctcgacga gatcgacctg cgaatcctgg tgtcaccgcc
     3721 ggtgagcggc gacgccggct gaccctgccg ggccggccgg gtgatcagtc gacccagtag
     3781 ccgtccacgc gggcgagggt gcgcaacatg acctcgtcgg cgccgccacc gatcgaccac
     3841 agacgagtgt cgcggaagaa ccgagccgtc caggtctcct ccatgtagcc gatgccaccg
     3901 tggaactgca ggcagatgtc ggcgaccttg cgagcgagac gggcgctgcg cagcttggcg
     3961 accgtggcga accgggtgat gtcctcaccg ttcgtctgcg ccaccgcagc gttgtagttg
     4021 tagtacttga gcaggtcgac ctcgctcgcc agatcggcga gctcgtaggc gaggtgctgg
     4081 ttggcgagca gcggcttacc gaacgccatg cgctgcttca ggtagtcggc cgtgcgctcg
     4141 agggcgtact ccatggcgcc caccatctgg tagctggcga tcatgcgctc gtcctggaac
     4201 tgcgacatct gctgctggaa gccacgaccg atctcgccga tcgtgttggc gaccggcacc
     4261 cgcatgttgt cgaagctgag ctcggcggtg tcggagctac gcatgcccaa cttgtcgagc
     4321 ttgcggctga cgatgaaccc gggagtgtcg gtcgggacga tcacctggga gacaccgcgc
     4381 gagccaccct cgtcggacgt gcgcacgagc aggcagaccc agtcggcctg ggtgccgctc
     4441 gtgatgtacg tcttcgcgcc gttgatgacc cactcgtcgc cgtcgcgggt ggccttggtg
     4501 cggatgcccg ccacgtcgga gccggcgtcg ggttcggtga cagccaccgc ggcgacgtgc
     4561 tgtccggaga tcgccggggc gaggaactgt tccttgagct cgtgggtgcc gaaacgagcc
     4621 agcgacgggg tcgccatgtc ggtctgcaca ccgatcgcca tcgcgacacc gaggcacggc
     4681 atccgaccga actcctcggc catgatcgcc acgtagccgt agtcggagcc gccaccgccg
     4741 taggcggggt cgtaggtgag cccgagcaca cccagctcgc cgagcttggg gaacaactcg
     4801 tgggccggga acgtgccggc ttcctcccac gcctcggcgt gggggttgat ctcacgctcg
     4861 acgaagtccc gcagggtctt gcggaacatg tggtgctctt cggtgaacag gctcactggt
     4921 actcccggaa tgtttcgttc tcgaccccag cctacgctgc agcgatgtcg tacgacgctc
     4981 ccaccccacc tccccccggc gaccccggcc gcaaccggac cccgtggctc gttgcgctcg
     5041 ccgccgtggt cgtcacgatc gtgatcgccg tcgccgtcgc gttcatgggc gggagcggcg
     5101 ataccggcaa gatcacgatc tcggccggag ggtcggccac cacgtcaccc gatggcgacc
     5161 gccccatcac gggtgaggtc gacgtcgacg gcgatgcgct gccgccgtac ctcgacggcg
     5221 agtccgacga cgccgtcggg gtcacgatcc cgacgatcac cggcaccgac tacgagggcc
     5281 gcccggtgac gatcgagccc ggcactcccc tgctgatcgt cgtgatggcc cactggtgcc
     5341 cccactgcca ggccgagatc ccgcgcatcg tcggctggaa cgacgacggc cgcgtccccg
     5401 aggagctcga cgtgatcggc gtgtcgaccg ccgtcgacga gacccgcggc aactacccgc
     5461 cctcggcgtg gctcgagcgg gagaactggc cgttccccgt gctcgccgac gacgaccgca
     5521 ccaccgcgct ccaggctctc ggctccagct cgttccccac gctgatcgcc gtcggcgccg
     5581 acggacgggt cgccctgcga gcgagcggcg agggcgacga ggaccgcttc gacgaactcg
     5641 tcgccgccgc gctcgaggga gcacccggcg acgccgaggc ccccgacacg accacgggtg
     5701 gagcgcccgc cggcagcgca gcctgtccgg cggccgacgg ttcgtccgcc aagcaacagg
     5761 agttcgacgg cccgccgccg atgtgcatcg actccgcagc gacctacacc gcggccgtcg
     5821 acacgacgat gggctcgttc accatcacct tcgaccccac catcgctccg gtgacggtca
     5881 acaacttcgt gttcctcgcc cgctaccact acttcgacgg catcatcttc caccggatca
     5941 tcgacggctt cgtgatccag ggcggcgacc ccgaaggcac cggcatgggc gggcccggct
     6001 acgagttcga agacgaactc ccgcggcaag gcagctacga gaagtactcg gtggccatgg
     6061 cgaatgccgg ccccgacacc aacggcagcc agttcttcgt catcaccggc cccgacggcg
     6121 aggccctccc gccgtcgtac tcgctgttcg gcaaggtcac cgccggcacc gaagtcatcg
     6181 acgccatctc ggcggtcccc accgacgtcc aggacgcccc cacgaccgct gtcgtgatca
     6241 acagcgtcgt gatcaccgag ggttcgtgac gtgagcgggg acgagcgcgg ggacgcgagc
     6301 ggcgagccgg ccccctgggc cccgttcgac gcctcgtcgg gtacccccgc ttcgccgggt
     6361 cccgctgccc cgtcgacgcc cccgtcgaca cccccgctgt accaaccccc cgcccacctg
     6421 ccaccctcgc acgtgccccc gtcgcacgtg cccccatcgc acctaccccc gccgcacctg
     6481 ccgccggggc tcgtgccggc cccgatgccg aactggggcc cgcccgtcga ccagcagaac
     6541 tacctccaac gccccggcgc cgccgagacc agggcgcgca atcgccgggc actcgtctac
     6601 gcactgatcg ccctcgtcgt cacggtggcg ctg
//
LOCUS       DAOSOA010000173         6607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11717_length_6607_cov_5.673687, whole genome shotgun sequence.
ACCESSION   DAOSOA010000173 DAOSOA010000000
VERSION     DAOSOA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11717_length_6607_cov_5.673687"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKY13_10950"
     CDS             complement(<1..491)
                     /locus_tag="PKY13_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT80497.1"
                     /translation="MNVPTCFDVTIDNGVAHLRLNRPDELNTMNVAFWSELPELIEQI
                     DAAASARAIVISSTGRHFSAGMDLSVFTGGGGLLGDPGVSETGRQRAHLWSMVQKLQD
                     SFTVLERVRMPVLAAIQGGCIGGAVDMVCAADMRYASADAFFCIQEINIGMTADVGTL
                     QRLP"
     gene            573..3098
                     /locus_tag="PKY13_10955"
     CDS             573..3098
                     /locus_tag="PKY13_10955"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOT80498.1"
                     /translation="MEPNNPHRLTRDVVPSAYRVAIDTDLGEARFTGTVAIDATVEQP
                     TDRIVCNAVDLTIGHASVRAGDHAIDATVELDADTERLILSLAEPVAVGDVVIELAFD
                     GVLNDQLVGFYRSSFTVDGRTEYMAVTQFEAPYARRAFPCWDEPEFKATFEISLTCDA
                     GLTAVSNGAETARTDLDDGRTRFDFAPTMKMSTYLVAWVIGELEVSDTVRGGTTDIRV
                     IHRPGQGHLTSFALECAAHSVAWFEEYYGIDYPGQKLDLVAVPDFAFGAMENLGCVTF
                     RENLLLIDPERSNRSEQERAATVIEHEIAHMWFGDLVTMQWWNGIWLNEAFATFMEFC
                     CADSFRPDWEIWTSFGLSKSQAFETDALAHTRPIEFEVVTPADAEGMFDILTYEKGAS
                     VLRMFERWAGADEFREGVRLYLARHAYANTDTPDLWESLAEATGVDVGAMMNTWILQG
                     GHPLVSVTSTGTGIRLEQQRFAFAGSDAETGSSWIVPVRVDASVSGERVERTVLLDGK
                     TLDIDLGGPVDWVNANAGFTGFYRTEYESGLRSALLAVVTDLESIDRYALLDDAWSLL
                     LADRLDLSDLAATLRAVAAVETEPSVWRRIAGILGSLSSLCLPSDRPAVAALVRELVG
                     PLLADDTTSPTVDHDLHAVLFRLAGTVGGLDSVIDEARRLYDGLPTSVDGNPDLGAAV
                     LDVVASKGTAADFDSLITMFRTATTPQEELRALSALAQFPDLDLTARLCEMCATEVRS
                     QNAPYVLAQAMANPVCGPQVWAFVAARWDELVARFPSSSVIRMAGGIRSFADPDLAAE
                     IAAFFETHEISQATLTLDQHLERLQVNVATRRRLGEGISGQLA"
     gene            3095..4249
                     /locus_tag="PKY13_10960"
     CDS             3095..4249
                     /locus_tag="PKY13_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT80499.1"
                     /translation="MIALRSGPTVFAVEDRGAQLTWRRVAAGSVRIVCGPVDLVVESD
                     GGPAAVDLDGLTPDTAYDLTVTTGDGTATSTFRTLPSPPGEELFRFATMSDLHLGATA
                     FGVRFTMVEAPGTVDHPIRCASAALSDLTRWGARHLVVKGDVVQSNEDSSWKLAADLL
                     GSATMPCDVLPGNHDVAHGGPDPFGVARRHGIDLVEELEIRDLPGIRLVLMNSAIVGI
                     DRGRWHHLVDGAAAAVATADGPAMLLVHHHPQPAPVPTHFPPGIDSITARRLLRAVHA
                     ANPAVIGSSGHTHRHRRRTEAGVPWSEVGSTKDFPGVWAGYVVHEGGIRQVVRRVSSP
                     DCLPWLDRTRRAAGGAWGWYSPGRLSDRCFTHDWPRATRPARSEPGDTSP"
     gene            complement(4185..4835)
                     /locus_tag="PKY13_10965"
     CDS             complement(4185..4835)
                     /locus_tag="PKY13_10965"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOT80500.1"
                     /translation="MNALTWTLLALTVVVGAVDWFAVHTERKPLEYVAKPATMLPLIG
                     AALALDPAVPAMRPWFVAALVCSLAGDVFLMLPKPEQFFVPGLASFLVGHVLYVIGLT
                     KGDLSTGGLLVGAVLTFTALVAIGPTIVKSAAEVDSRLGVPVLLYMLTISVMVTCAIG
                     AGPWAGIVGALLFYGSDFCIGWSRFVQDFRHSHLVIMVTYHLAQIGLVASLVASHG"
     gene            complement(4832..6103)
                     /locus_tag="PKY13_10970"
     CDS             complement(4832..6103)
                     /locus_tag="PKY13_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT80501.1"
                     /translation="MSLDLDRVVIVGGSLAGFTAAATLRGEGWDGDLIVVEASATMPA
                     DRPPLSKQVLAGTTSPADAVSALTARIDGLDVDVRLDHRVAGAGFAADDLRLKFFNDH
                     EITADAIIVATGSAPRWPSGFLVETEESTANEPKFRPSLTGVHVVRDLPDAEALKADL
                     DAGPQRLVVIGAGFIGAEVAATARAAGIDVTIVEFAPLPLARVFGGLVGEFIAQLHRD
                     EGVDLRLGVGLDQIVGGADGRVAGVTLTDGSALDCDLLVLGLGAAPDLEWAIGSGLTI
                     DNGVVCDDTLSAGPGVVAAGDVANWHNPLFGERMRVEHWENAIEQGEAAAKRLLAARD
                     DVPGVPFASVPWFWSDQYDVKIQMAGRPAADDEIVLIDGDPAERRFAVAFRRGDRCTG
                     VFAANRPRIAVVARMRMRESLDWSHVVPTAS"
     gene            complement(6110..6304)
                     /locus_tag="PKY13_10975"
     CDS             complement(6110..6304)
                     /locus_tag="PKY13_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOT80502.1"
                     /translation="MKVVVDYDVCESNAVCMAVVPEVFEVRDDDFLYVLEEHPGEELR
                     AKVEEAVKRCPKQAISIEED"
     gene            complement(6384..>6607)
                     /locus_tag="PKY13_10980"
     CDS             complement(6384..>6607)
                     /locus_tag="PKY13_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT80503.1"
                     /translation="IRANSLIPGWTETDLTQPLLGWEKFMAATTARTPVRRWGLPSDM
                     GPVAVFLADPTLTFHTGDCVVVDGGYTVF"
BASE COUNT         1058 a         2363 c         2218 g          968 t
ORIGIN      
        1 ggcaggcgct ggagcgtgcc gacatcggcg gtcatgccga tgttgatctc ctggatgcag
       61 aagaaggcgt cggcgctggc gtaccgcatg tcggcagcgc acaccatgtc gacggcccca
      121 ccgatgcacc cgccctggat cgcggccagg acgggcatac gaacccgttc gagcacggtg
      181 aacgagtcct gcagcttctg caccatcgac cacaggtgcg cccgctgccg gccggtctcg
      241 ctcacccccg gatcgcccaa gaggccacca ccaccggtga acaccgacag gtccatgcct
      301 gccgagaagt gccgaccggt cgaactgatg acgatggccc gggccgacgc cgcggcgtcg
      361 atctgctcga tcagctcggg caactcgctc cagaacgcca cgttcatggt gttgagctcg
      421 tcaggccggt tgaggcgcag gtgggcgaca ccgttgtcga tggtgacgtc gaagcaggtg
      481 ggcacgttca tctccctggg gtgagcggcg ccgggaaggt cggtgccaga gcacagtggc
      541 caaatggtac ccccgctacg ctgagtaccg tcatggaacc caacaacccg caccggctca
      601 cccgcgacgt cgtcccgtcc gcctaccgag tcgccatcga caccgacctc ggcgaggccc
      661 gcttcacggg aacggtggcc atcgacgcga ccgtcgagca gccgaccgac cgcatcgtgt
      721 gcaacgccgt cgatctcacg atcgggcacg catcggtccg cgccggcgac cacgccatcg
      781 acgcgaccgt ggaactggac gccgacaccg agcggctgat cctctcgctg gccgagcccg
      841 tggcggtcgg tgacgtggtg atcgagctcg cgttcgacgg agtgctcaac gatcagctgg
      901 tcggcttcta ccgttcgtcg ttcaccgtcg acggccgcac cgagtacatg gccgtcaccc
      961 agttcgaggc gccgtacgcc cgacgggcgt tcccctgctg ggacgaaccc gagttcaagg
     1021 cgaccttcga gatcagcctc acgtgcgacg ccggtctgac cgcggtgtcg aacggtgccg
     1081 agaccgcacg caccgatctc gacgacggcc gcacccggtt cgacttcgcc cccaccatga
     1141 agatgtcgac gtacctcgtc gcatgggtca tcggcgagct cgaggtcagc gacacggtcc
     1201 gcgggggtac gaccgacatc cgggtcatcc accgtccggg gcaagggcac ctgacgtcgt
     1261 tcgcactcga gtgcgccgct cactccgtgg cgtggttcga ggagtactac ggcatcgact
     1321 acccgggcca gaagctcgac ctggtcgccg tgcccgactt cgcgttcggt gcgatggaga
     1381 acctcgggtg cgtcaccttc cgcgagaacc tgctgctcat cgatccggaa cgatcgaacc
     1441 ggtccgagca ggagcgggcg gcaacggtca tcgagcacga gatcgcgcac atgtggttcg
     1501 gcgacctcgt gacgatgcag tggtggaacg gcatctggtt gaacgaggcc ttcgccacgt
     1561 tcatggagtt ctgctgcgcc gactcgttcc gtcccgactg ggagatctgg acgtcgttcg
     1621 gcctctcgaa gtcgcaggcg ttcgagaccg acgcgctcgc ccacacccgt ccgatcgagt
     1681 tcgaggtcgt gacgcccgcc gacgccgagg gcatgttcga catcctcacc tacgagaagg
     1741 gcgcttcggt cctccggatg ttcgagcggt gggccggcgc cgacgagttc cgcgagggcg
     1801 tccgcctcta cctcgcccgg cacgcctacg cgaacaccga cacccccgat ctgtgggagt
     1861 cgctcgcgga ggcgacgggt gtcgacgtcg gggcaatgat gaacacctgg atcctccagg
     1921 gcggccaccc cctggtgtcg gtcacgtcca ccggcacggg tatccggttg gagcagcagc
     1981 ggttcgcgtt cgccgggagc gacgccgaga cgggcagctc ctggatcgtc cctgtccgcg
     2041 tcgacgcctc ggtgagcggc gaacgcgtcg agcgcacggt gttgctcgac ggcaagacgc
     2101 tcgacatcga cctcgggggc ccggtcgact gggtgaacgc gaacgctggc ttcacgggct
     2161 tctaccgaac cgagtacgag tccggtctgc ggtcggccct gctcgcagtg gtcaccgatc
     2221 tcgagtcgat cgaccgctac gcactgctcg acgacgcctg gtcgctgttg ctcgccgacc
     2281 ggctcgacct gtccgacctc gcagcgacgc tccgggccgt ggcagcggtc gagaccgagc
     2341 cgtcggtgtg gcggcgaatc gccggcatcc tcggcagcct ctcctcgttg tgcctcccgt
     2401 cggaccgccc cgccgtggcg gcgctggtgc gggagctcgt tggtccgctc ctggccgacg
     2461 acacaacgtc gccgaccgtc gaccacgacc tccacgcggt gctcttccga ctggccggca
     2521 ccgtcggagg gctcgactcc gtcatcgacg aggcccgccg cctctacgac gggctgccga
     2581 cgtcggtcga cggcaacccc gacctcggcg ccgcggtgct cgacgtcgtg gcgagcaagg
     2641 gcacggccgc cgacttcgac agcctgatca cgatgttccg aactgcgacg accccacaag
     2701 aagagttgcg ggcactgtcc gcgctggcac agttccccga tctcgacctg acggctcggc
     2761 tctgcgagat gtgcgccacc gaggtccgca gtcagaacgc cccctacgtg ctcgcccagg
     2821 caatggccaa ccccgtctgc ggcccgcagg tgtgggcctt cgtcgccgca cgctgggacg
     2881 agctcgtcgc acggttcccg tcgtcgagcg tgatccgcat ggccggcggg atccgttcct
     2941 tcgccgaccc cgacctcgcc gccgagatcg ccgcgttctt cgagacccat gagatctccc
     3001 aggcgacgct gacgctcgac cagcacctcg agcggctgca ggtgaacgtc gcgacccgcc
     3061 ggcggctcgg cgaagggatc agcggccagc tggcatgatc gctctccgat ccggcccgac
     3121 ggtgttcgcc gtcgaagacc gcggtgcaca actgacgtgg cggcgcgtcg cggccggatc
     3181 ggttcggatc gtgtgcggcc ccgtcgacct cgtggtcgag tccgatggtg ggccggcggc
     3241 ggtcgacctc gacggcctca cccccgacac ggcgtacgac ctgaccgtca ccaccggcga
     3301 cggcacggcg accagcacgt tccgcaccct cccgtcaccg ccgggtgagg aactgttccg
     3361 gttcgcgacc atgagcgacc tgcacctcgg cgccaccgcc ttcggggttc gattcacgat
     3421 ggtcgaggcg ccgggcaccg tcgaccaccc gatccgctgc gcgtccgcag cgctcagcga
     3481 cctgacacgg tggggcgccc gccacctcgt cgtgaagggt gatgtcgttc agagcaacga
     3541 ggacagctcg tggaagctcg ccgccgacct gctcggctcg gcgacgatgc cgtgcgacgt
     3601 cctccccggc aaccacgacg tcgcccacgg cggaccggac cccttcggcg tggcccggcg
     3661 acacggcatc gacctcgtcg aggagctcga gatccgcgac ctccccggca tccgcctggt
     3721 cctcatgaac tcggcgatcg tcggcatcga ccggggccgg tggcaccacc tcgtcgacgg
     3781 cgccgccgcg gccgtcgcca ccgccgacgg tccggcgatg ctcctcgtcc accaccaccc
     3841 ccagcccgcc ccggtgccaa cgcatttccc gcccggcatc gactcgatca ccgcccgccg
     3901 acttctccgg gcggtgcacg ccgccaaccc cgccgtcatc ggttcgtccg gacacaccca
     3961 ccggcaccga cgccggaccg aggccggcgt gccgtggtcg gaggtcggtt ccaccaagga
     4021 cttccccggc gtgtgggccg gctacgtcgt gcacgaaggc ggcatccgtc aggtcgtcag
     4081 gcgggtgagc tcgcccgact gccttccgtg gctcgatcgc acgcgccgag ccgccggcgg
     4141 cgcctggggc tggtactcac cgggccgatt gtcggaccgg tgcttcaccc atgactggcc
     4201 acgagcgacg cgaccagccc gatctgagcc aggtgatacg tcaccatgat cacgaggtgg
     4261 gagtgccgga aatcctgcac gaacctcgac cagccgatgc agaagtccga gccgtagaac
     4321 aacagggcgc cgacgatgcc cgcccatggg ccggcaccga tcgcgcaggt caccatcacc
     4381 gaaatggtga gcatgtacag gaggaccggc accccgaggc gggagtcgac ctcggccgcg
     4441 ctcttcacga tcgtcggacc gatcgcgacg agcgccgtga acgtgagcac cgcccccacg
     4501 aggaggccgc ccgtcgacag gtcgcctttc gtcagtccga tcacgtacaa cacgtggccg
     4561 accaggaacg aggcaagtcc gggaacgaag aactgctccg gcttcgggag catgaggaac
     4621 acgtcgccgg cgagcgagca cacgagcgcc gccacgaacc agggccgcat cgccggcact
     4681 gccgggtcga gggccagtgc agcgccgatc agcggcagca tcgtcgcggg cttggcgacg
     4741 tactcgagcg gcttccgctc ggtgtggacc gcgaaccaat cgacggcgcc gacgacgacg
     4801 gtgagcgcga gcagcgtcca ggtgagagcg ttcacgaggc ggtcggcacg acgtgggacc
     4861 agtcgagcga ctccctcatg cgcatacggg ccaccaccgc gatgcggggc cggttcgccg
     4921 cgaacacgcc ggtgcaccgg tcgccgcgac gaaaggccac cgcgaaccgg cgttcggccg
     4981 gatcgccgtc gatgagcacg atctcgtcgt cggcggcggg gcgcccggcc atctggatct
     5041 tgacgtcgta ctgatcggac cagaaccacg gcaccgaggc gaacggcacg cccggcacgt
     5101 cgtcacgagc ggccaggagc cgcttcgccg cggcctcgcc ctgttcgatg gcgttctccc
     5161 agtgttcgac gcgcatgcgt tcgccgaaca gggggttgtg ccagttggcg acgtcaccgg
     5221 cggcgaccac ccccggtccg gccgacaacg tgtcgtcgca gacgacgccg ttgtcgatcg
     5281 tcaggcccga tccgatggcc cactcgaggt cgggcgcggc gccgaggccg agcaccagga
     5341 gatcgcagtc gagggcagag ccatcggtca gggtgacccc ggcgacgcga ccgtcggccc
     5401 cgccgacgat ctggtcgagc cccacgccga gacggaggtc gacgccctcg tcgcggtgca
     5461 gctgcgcgat gaactcaccg acgaggccac cgaacacgcg ggccagcggc aacggtgcga
     5521 actcgacgat cgtgacgtcg atgcctgcgg cacgggccgt ggccgcgacc tcggccccga
     5581 tgaacccggc gccgatcacg acgagacgtt gggggccggc gtcgaggtcg gctttgagcg
     5641 cctcggcgtc agggaggtcc cgcacgacgt ggaccccggt cagcgacggc cggaacttcg
     5701 gctcgttggc ggtcgattcc tcggtctcga cgaggaatcc ggacggccac cggggcgccg
     5761 agccggtcgc cacgatgatc gcgtcggcgg tgatctcgtg gtcgttgaag aacttgaggc
     5821 gcaggtcgtc ggcagcgaaa ccggcaccgg cgacgcgatg atcgagccgg acgtcgacgt
     5881 cgagaccgtc gatgcgcgcg gtcagcgccg acacggcgtc ggccggcgag gtcgtgccgg
     5941 cgaggacctg cttggacagc ggcgggcgat cagcgggcat cgtcgccgat gcctcgacga
     6001 cgatcaggtc gccgtcccac ccctcgcccc gcagcgtggc cgcggccgtg aaccccgcca
     6061 gcgacccacc gacgatgacg acgcggtcga ggtcgaggga caacggcggt cagtcctctt
     6121 cgatggagat ggcctgcttg gggcagcgct tcactgcctc ttcgaccttg gcgcgcagtt
     6181 cctcgcccgg gtgctcctcg aggacgtaca ggaagtcgtc gtcgcgcacc tcgaacacct
     6241 cgggcaccac ggccatgcac accgcgttgg actcacacac gtcgtagtcg acgacaacct
     6301 tcatgctgca cccctcatgt ctgcgagcgt tcggcggtca ctctaacccg agcgggggtg
     6361 gggtgaatcg ctgcgccacc ggatcagaag acggtgtaac cgccgtcgac aacgacgcag
     6421 tcaccggtgt ggaacgtgag ggtcgggtcc gccaggaaga cggccacggg ccccatgtcg
     6481 gagggcagtc cccaccgtcg cacgggtgtc cgggcggtgg tcgccgccat gaacttctcc
     6541 caccccagca acggttgggt gaggtcggtc tcggtccagc ccgggatgag cgagttggca
     6601 cggatgc
//
LOCUS       DAOSOA010000174         6600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11734_length_6600_cov_5.218946, whole genome shotgun sequence.
ACCESSION   DAOSOA010000174 DAOSOA010000000
VERSION     DAOSOA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11734_length_6600_cov_5.218946"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1344)
                     /locus_tag="PKY13_10985"
     CDS             complement(124..1344)
                     /locus_tag="PKY13_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT80504.1"
                     /translation="MQRDLERIFREERGRVTATLIRLFGDIDVAEEAVQEAFLVATQR
                     WPETGLPPNPGGWILTTARNRAIDRVRRESTRHDRHVAAHRLAEPDDRPPDPREEGPV
                     QDDQLRLMFTCCHPALATGAQVALTLRLLGGLETTEIARAFLVPEATIAQRIVRAKRK
                     IRDANIPYRVPRDADLPDRTRSVLAVVYLVFNEGYTASAGDALGRDDLCAEAIRLGRL
                     LAELMPDEPEVLGLLSLMLLVASRRPARTAADGSIVLLADQDRTAWDHTMAAEGRALA
                     AACLRRGVPGTYQLQATINAVHSEAVTSADTDWAQIVRIYDHLLAIAPSPVVALNRAV
                     AVAEVDGPAAALALVDELDLDGYHLVHATRADLLARLGRVAESASAYERAESLATNAA
                     EQRFLRERRAALSG"
     gene            complement(1344..1712)
                     /locus_tag="PKY13_10990"
     CDS             complement(1344..1712)
                     /locus_tag="PKY13_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOT80505.1"
                     /translation="MKQYMLSVHHDSKDAFEGRDLTPDDFQRMFEQVDAFNQDVTAKG
                     AWVFGGGLTLPDTATTVRNKDGELLLTDGPYAETKEVLGGFWVIQVADLDEALEWAKK
                     ASLACEGDVEVRPFQEEPED"
     gene            complement(1792..2532)
                     /locus_tag="PKY13_10995"
     CDS             complement(1792..2532)
                     /locus_tag="PKY13_10995"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HOT80506.1"
                     /translation="MESVSPLVDWLRTDEAKSRLAMAVTLSATSAFPVALLALRARLG
                     DGLGYRFLLWNLFLAWIPLGFAFVAELGWRRHWSRRSLLWPLFGWLLFFPNAPYVITD
                     VIHLRKNEFSPLWFDALILFSTGLAGLLVGFVSLRMVQLIVNASFSRRIGWVVSVGVI
                     MLSGFGIYLGRFGRYNSWDVLSSPRNLLYDVQSVALNPVSNGRAIAISVIFSAFLAVT
                     YLGMIALGQLRLPWRPPAPFPEESEKSS"
     gene            2586..3302
                     /locus_tag="PKY13_11000"
     CDS             2586..3302
                     /locus_tag="PKY13_11000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOT80507.1"
                     /translation="MTPPASPTADPALPQGEAKIAAVRSMFDTIAPRYDLVNRLMTFR
                     MDVGWRQRAVSSLGLVPGSVVADLACGTGDFCFELQRKGMVPLGFDLSFGMLAAAPRN
                     FPRAQADVLRLPLADGVLDGVTCGFALRNLVELDGFFAELARVVRPGGRIALLDVAQP
                     PNPLLRFGHGIYFGKVVPLVGGLLSDKQAYAYLPKSVAYLPEPEEMVSSLQRHGFADA
                     RRSLFTVGISQLFSGTRDRT"
     gene            3302..4525
                     /locus_tag="PKY13_11005"
     CDS             3302..4525
                     /locus_tag="PKY13_11005"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HOT80508.1"
                     /translation="MEATSRRANGRPDAPGLPTTMFLSPRRELRGWGEAFRIEVPAPW
                     VEHLDVVTAALAAVTPGAASDVGDRPASGPVAFAALPFDRDAAATFVVPALVHVSTAE
                     GTHWVTEIASPPVLSRESVQNESLRGTEPVAGPTELSIRSTISADEWCDLVDEATKRI
                     ASGDLQKVVLARELVVTADAPFDAHEVAHRLAAAHPHAFRFDVDGFVGASPELLVSRF
                     GDVVRAHPMAGTTPRSGDPEIDQRQAAELLHSAKNRAEHQITIDMVLDTLLGWSSFVD
                     AAPAPAVVAAGSVQHLATLVEGRLSEPPASVLELVAALHPTPAVGGYPREAALDLIAE
                     LEPDPRGRYAGPVGWVDAAGNGAWAVGLRSASLDGTTARLFAGVGVVADSDPRSELAE
                     TQAKFAATLGSITRI"
     gene            complement(4512..6263)
                     /gene="menD"
                     /locus_tag="PKY13_11010"
     CDS             complement(4512..6263)
                     /gene="menD"
                     /locus_tag="PKY13_11010"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HOT80509.1"
                     /translation="MVPDTPAPDVSDAGEPAADNAGITFCATLVDQWVRHGVRHAVVS
                     PGSRSTPLALACANHPGLRTHVHHDERSASFMALGIGLATRVPALLVTTSGTAVAELH
                     AAVVEAHQACVPMLVCSADRPPELQGVHAPQTIDQTRIFGEAVRWFVEPGPPSDDFRQ
                     AWRHLAADAFGAAMGATLGHPPGPVHLNLAFREPLLGPLGQLPDPVDLEPAVPALWGL
                     LDEQLGQLARAMSGTRGVIVAGDRAVVEDGDREAIHTLAVVTGWPILADHLSGLRGDE
                     RAVTAFDSMLRVDELAAELRPEFVLRIGGLLASRVTNEWLAASGATHVGLDRWGVIPD
                     PDHVVGERLVADILLVCDQLAQASPAPAPEEWTRRWYDAERSARRAIIEERAGEPLVT
                     NTALTKVPPGGNLVVSSSMPVRDLEWYGDRRGDIAVYSNRGANGIDGVTSTAVGVALG
                     SGKPTVLVIGDVAFVHDTNGLLGMGRREVPLCIVVIDNDGGGIFSFLPQGTELDHERF
                     EQLFGTPHAVDLESLARAHGLDFVRTYRHDDIVAALEKWVAFPRPLVVFARSERRRNV
                     VDHTAINAAVAEALRSA"
     gene            6296..>6600
                     /locus_tag="PKY13_11015"
     CDS             6296..>6600
                     /locus_tag="PKY13_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT80510.1"
                     /translation="MSSPSPVVLLHGFGQNGRCWGRLGESLAAVGEVTALDLPGHGAA
                     GAVQADLWTTADLVAEAIDPERPAAVVGYSMGGRIALHTALAHPERVGRLVLISATAG
                     "
BASE COUNT          990 a         2381 c         2308 g          921 t
ORIGIN      
        1 tgcagcacgc ctgcgagcgg ggcggcaccg ccgacgagat tgccgagcac ctccgagaac
       61 gcatcgaggc ggtcctgccg ttggcgctcc gcaacgacga catcgccgtg ttggtgctgc
      121 ggatcacccg ctgagcgccg cccgacgttc ccggaggaac cgctgctcgg ccgcgttggt
      181 cgccaaggac tcggcgcgct cgtaggcgga cgcactctcg gcgacgcggc cgaggcgggc
      241 gagcaggtcg gcgcgggtgg cgtgcacgag gtggtagccg tcgaggtcga gctcgtcgac
      301 cagcgccaac gccgcggccg ggccgtcgac ctcggcgacg gcgaccgccc ggttcagcgc
      361 gacgaccggg gacggggcga ttgcgagcag gtggtcgtag atccggacga tctgggccca
      421 gtccgtgtcg gccgacgtca ccgcctcgct gtgcacggca ttgatcgtcg cctgtagctg
      481 gtaggtacca ggcacccctc gacgaaggca cgccgccgcg agggcccgac cctcggccgc
      541 catcgtgtgg tcccaggcgg tgcggtcctg atcggcgagc aggacgatcg agccgtcggc
      601 agccgtccgg gccggacgac gcgacgcgac cagcagcatc agcgacagga ggccgagcac
      661 ctcgggttcg tccggcatca gctctgcgag cagacgaccg agccggatcg cctcggcgca
      721 caggtcgtcg cgcccgagcg cgtcgcccgc cgaagccgtg tacccctcgt tgaacacgag
      781 atacaccacc gccaggaccg accgcgtgcg gtcgggcagg tcggcgtcac gcggcacgcg
      841 gtaggggatg ttggcgtcgc ggatcttgcg cttggcccgc acgatccgct gggcgatcgt
      901 cgcctcgggc acgaggaacg cccgggcgat ctcggtggtc tcgaggcccc cgagcaggcg
      961 gagggtgagc gccacctggg cgccggtcgc caacgccgga tgacagcagg tgaacatgag
     1021 tcgcagttgg tcgtcctgca cgggaccctc ctctcgggga tcaggcggcc gatcgtcggg
     1081 ttcggcgagg cgatgggcgg ccacgtggcg gtcgtggcgt gtcgactcac gccggacgcg
     1141 gtcgatcgcc cggttccggg ccgtcgtcag gatccagccg cccgggttcg gcggcagccc
     1201 ggtctcgggc caccgctggg tcgcgaccag gaacgcctcc tggaccgcct cctcggcaac
     1261 gtcgatgtcg ccgaagaggc ggatgagcgt ggcggtgacc cgaccccgct cctcccggaa
     1321 gatccgctcg aggtcgcgct gcatcagtcc tcgggttcct cctggaacgg ccgcacctcg
     1381 acgtcgccct cgcacgccag cgaggccttc ttggcccact cgagcgcctc gtcgagatcg
     1441 gcgacctgga tcacccagaa cccgccgagg acctccttgg tctccgcgta gggaccgtcg
     1501 gtgagcagca gctcgccgtc cttgttacgc acggtcgttg cggtgtcggg cagcgtgagc
     1561 ccgccgccga acacccaggc gcccttcgcg gtcacgtcct ggttgaacgc gtcgacctgc
     1621 tcgaacatgc gctggaagtc gtcgggcgtg aggtcccgac cctcgaaggc atccttcgag
     1681 tcgtggtgga cggacagcat gtactgcttc atggggatct cctcctgtga ggaccggcac
     1741 cgtaccggtt cctgcactct ctacgaacga ccgacacgtc gatcgacggg ttcaggaaga
     1801 tttctcggat tcctcgggaa acggggccgg cgggcgccac ggcagccgga gctggccgag
     1861 cgcgatcatc ccgaggtacg tcaccgcgag gaacgccgag aagatgacgc tgatggcgat
     1921 cgcccgcccg ttcgacaccg ggttcaacgc caccgactgc acgtcgtaca gcaggttgcg
     1981 ggggctcgag aggacgtccc agctgttgta gcgcccgaac cggcccaggt agatgccgaa
     2041 gccactcagc atgatcaccc cgaccgacac gacccacccg atgcgacggc tgaacgacgc
     2101 gttgacgatc agctggacca tgcggagcga cacgaacccc acgagcagac cggcgaggcc
     2161 cgtcgagaac aggatgagcg cgtcgaacca gagcggcgag aactcgttct tccgcaggtg
     2221 gatgacgtcg gtgatgacgt agggggcgtt cgggaagaac agcagccacc cgaacagcgg
     2281 ccacaggagg ctccggcgcg accagtgccg acgccagccg agctcggcga cgaacgcgaa
     2341 gccgaggggg atccaggcca ggaacaggtt ccacaagagg aatcggtacc cgagcccgtc
     2401 ccccagtcgt gcccgcagcg cgaggagggc gaccgggaac gccgaggtcg ccgacagtgt
     2461 gacggccatc gccaggcgtg acttcgcctc gtcggtccgc aaccagtcga cgaggggcga
     2521 caccgattcc accggcccca cggtaccggt gcagggccgt cgcaccgagg ccataggctc
     2581 acagcgtgac tcccccagcg tcccccaccg ccgatccggc cctcccgcag ggcgaggcca
     2641 agatcgccgc cgtccggtcg atgttcgaca cgatcgcgcc gcggtacgac ctcgtgaacc
     2701 gcctcatgac attccggatg gacgtggggt ggcgccagcg ggccgtgtcg tcgctcggcc
     2761 tcgtgcccgg ctcggtcgtg gccgacctgg cgtgcgggac cggcgacttc tgcttcgagc
     2821 tccagcgcaa ggggatggtc ccgctcgggt tcgacctcag cttcgggatg ctggccgccg
     2881 ctcctcgcaa cttcccccgg gcgcaggccg acgtgctgcg tctgccgctg gccgacggtg
     2941 tgctcgacgg cgtcacgtgc ggcttcgcgc tgcggaacct ggtcgagctc gacgggttct
     3001 tcgccgagct ggcccgtgtg gtccgccccg gcggccgcat cgcgctcctc gacgtggcac
     3061 agccgcccaa cccgctcctc cgcttcgggc acggcatcta cttcggcaag gtcgtgccgc
     3121 tggtcggcgg gctgctgtcg gacaagcagg cctacgccta cctccccaag tcggtcgcct
     3181 acctgcccga gcccgaggag atggtgtcgt cgctgcagcg ccacggcttc gccgacgcgc
     3241 ggcggtcgct cttcaccgtc ggcatcagcc agctgttcag cgggacccgg gaccgcacct
     3301 gatggaggcg accagtcggc gggccaacgg tcgtcccgac gccccgggcc tgccgacgac
     3361 catgttcctc tcaccgcgcc gcgagctgcg ggggtggggc gaggcattcc gcatcgaggt
     3421 cccggcgccg tgggtcgagc acctcgacgt cgtgaccgcc gcgctcgccg ccgttactcc
     3481 cggtgccgcc tccgacgtcg gcgaccgccc cgcctcgggc ccggtcgcgt ttgccgcgct
     3541 gcccttcgac cgcgacgccg cagcgacttt cgtcgtcccc gcactcgtgc acgtgtcgac
     3601 ggccgagggc acccactggg tcacggaaat cgcgtcccca cccgttttgt cccgcgaaag
     3661 cgtgcagaac gaatcgcttc gcgggacaga accggtggcg gggccgacgg aactgtcgat
     3721 ccgatcgacg atcagtgccg acgagtggtg cgacctcgtc gatgaggcga ccaagcgcat
     3781 cgcctcaggc gacctccaga aggtcgtcct ggcgcgtgaa ctggtcgtca ccgccgacgc
     3841 accgttcgac gcccacgagg tcgcacatcg tctggcggcg gcacatcccc acgcgttccg
     3901 cttcgacgtc gacgggttcg tgggggcgag ccccgagctg ctcgtgtcgc gcttcggcga
     3961 cgtcgtccgc gcccacccca tggccggcac gaccccgcgc agcggcgatc ccgagatcga
     4021 ccagcgccag gccgccgagc tcctgcattc ggccaagaac cgcgccgagc accagatcac
     4081 gatcgacatg gtgctcgaca cgttgctcgg ctggtcgagc ttcgtcgacg ccgccccggc
     4141 gcccgcagtc gtcgccgccg gcagcgtcca gcacctcgcc acgctcgtcg agggccggtt
     4201 gagcgagccg ccggcatcgg tcctcgagct ggtcgccgca ctgcacccga cccccgcggt
     4261 cggcggatac ccccgcgagg ccgcgctcga cctgatcgcc gagctcgaac ccgacccccg
     4321 cgggcgctac gccggccctg tcggctgggt cgacgccgcg ggcaacggtg cctgggccgt
     4381 cggtctccgc agcgcgtcgc tcgacggaac gaccgcccgc ctgttcgccg gggtcggggt
     4441 ggtcgccgac agcgaccccc gcagcgaact cgccgagacg caggccaaat tcgccgccac
     4501 cctcgggtcg atcacgcgga tctgagcgcc tcggcgaccg cagcgttgat ggcggtgtgg
     4561 tcgacgacat tgcgccgacg ctcactccgg gcgaacacga ccagcggccg agggaacgcc
     4621 acccacttct cgagggcagc gacgatgtcg tcgtgccggt acgtccgcac gaaatcgagg
     4681 ccgtgcgccc gggccaacga ctcgagatcg acggcatggg gcgtgccgaa gagctgttcg
     4741 aaccgctcgt ggtcgagttc cgtcccttgg ggaaggaacg agaagatgcc gccgccgtcg
     4801 ttgtcgatca ccacgatgca cagcggcacc tcgcgtcgac ccattccgag cagcccattg
     4861 gtgtcatgga cgaaggcgac gtcaccgatc accagcacgg tcggtttgcc actcccgagc
     4921 gcaaccccga ctgccgtcga ggtgaccccg tcgatgccgt tggccccgcg gttcgagtac
     4981 acggcgatgt cgccccgccg gtcgccgtac cactcgagat cacgcaccgg catcgaggac
     5041 gacacgacga gattgccacc gggcggcacc ttggtgagcg ccgtgttggt gacgagcggc
     5101 tcgcccgccc gctcctcgat gatggcccgc cgcgccgagc gttcggcgtc gtaccagcgg
     5161 cgcgtccact cctcgggcgc cggggccggc gaggcctggg cgagctgatc gcacaccagg
     5221 aggatgtcgg cgacgagccg ttcgccgacc acgtgatcgg gatcggggat gacgccccac
     5281 cggtcgagcc cgacgtgcgt tgcacccgac gcggccaacc actcgttggt gacacgcgac
     5341 gccagcagcc caccgatgcg caacacgaac tccgggcgca gctccgcggc caactcgtcg
     5401 acgcgcagca tcgagtcgaa cgccgtgacg gcgcgttcgt cgccgcgcag acccgacagg
     5461 tggtcggcca ggatcggcca gccggtgacg acggcaagcg tgtggatcgc ctcgcggtca
     5521 ccgtcctcga ccacggcccg atcgccggcg acgatcacgc cgcgcgtccc cgacatggcc
     5581 cgggcgagct gcccgagctg ctcgtcgagg aggccccaca gcgccggcac cgccggttcg
     5641 aggtcgaccg gatcgggcag ttgcccgagc ggtccgagga gtggctcgcg gaacgcgagg
     5701 ttcagatgaa cgggcccggg cgggtgaccg agcgtcgccc ccatcgccgc tccgaacgcg
     5761 tcggcggcca gatgccgcca cgcctgccgg aagtcgtcgg acggcgggcc gggctcgacg
     5821 aaccagcgga cggcctcgcc gaagatgcgg gtctggtcga tcgtctgggg ggcgtggacg
     5881 ccctggagct caggaggccg atcggcactg cacaccaaca tcggcacaca cgcctggtgc
     5941 gcctccacga cggcggcgtg cagttcggcg acggcggtgc cgctcgtcgt gaccaacagg
     6001 gccggaaccc gggtggcgag cccgatgccg agcgccatga acgaggccga gcgctcgtcg
     6061 tggtgcacat gggtgcgcaa cccggggtgg ttcgcgcacg ccaacgccag cggggtcgac
     6121 cgggagcccg gtgacacgac ggcgtggcgg acgccgtgcc ggacccactg gtcgacgagg
     6181 gtcgcgcaga aggtgatccc ggcgttgtcg gcggccggtt ctcccgcatc ggaaacgtca
     6241 ggggcggggg tgtcggggac cattccgggc agcctacggc tacggtctcc cggcgatgag
     6301 cagcccctcc cccgtcgtcc tgttgcacgg tttcggtcag aacggccggt gttggggtcg
     6361 cctgggcgaa tcgctcgccg ccgtgggcga ggtgaccgca ctcgacctcc ccggtcacgg
     6421 cgccgccggc gccgtgcagg ccgatctctg gacgaccgcc gatctcgtcg cggaggccat
     6481 cgacccggag cgaccggctg ccgttgtcgg ttattcgatg ggtggccgca tcgcgctcca
     6541 cacggcgctg gcccaccccg agcgggtcgg gcgactggtg ctgatcagcg cgacggccgg
//
LOCUS       DAOSOA010000175         6597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11743_length_6597_cov_7.554418, whole genome shotgun sequence.
ACCESSION   DAOSOA010000175 DAOSOA010000000
VERSION     DAOSOA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11743_length_6597_cov_7.554418"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PKY13_11020"
     CDS             <1..1016
                     /locus_tag="PKY13_11020"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOT80511.1"
                     /translation="AQVFYAMKANPHPAVIGLLATMGCGIDAASTNEVDVAVAHGVPP
                     ERISYGNTVRRASEIEHARDAGVAMFAVDDDHELTKVLDHAPGSTVLARLNTEGTGAA
                     WPLSRKFGTDVPTAQSMLVRAQSRGHGVGATFHVGSQQSDPSSWDDALVQVAQLLEVV
                     EVTPDTPFTVNLGGGLPSRLADPTPALAEFSEVIHAAIARHLDGAPISFAIEPGRFLV
                     GDAGVIEAEVIGVVERPGQACSRWVFIDVGLFQGLSEASGESIRYPLSTTVGGPTSPA
                     VVAGPTCDSTDILYERRPVELPLALTSGDRIRLHGAGAYTVSCSSLGFNGFEPLRVEI
                     APS"
     gene            1051..2313
                     /locus_tag="PKY13_11025"
     CDS             1051..2313
                     /locus_tag="PKY13_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOT80512.1"
                     /translation="MARSRHAAGGLRPDRLLRRLVELLGPTGSAARQSLVALGFNSAT
                     SFAAGAMLVGFEDTWSRLSPMLILVPAAIGLRGNVFSTLGNRLSTSIHTGTFRVSFKA
                     DSVLGQNLLASFSLTLVMSVILAGFAKVLAVALGVVQHVSFLELAMVSALGGILGSVV
                     VAAATVLLTIGAVRFEWDLDNLVAPTVSTLGDVITIPALYLAAQLIGRGSVANVLGGV
                     VVVITAVVAVMSWRTGLELFKEIFRESLPVLAAALVLSALAGLVLQKQQNLLHVLPAL
                     GILQPAFVSSAGALGGILCGRIATNLHIGSVEPTLAPGAEARRDFSLVFGLAVPLLLF
                     NALGAWLVSLLTGSSGAPGFGWLLSTSLLAAIVTMAFVAALAYYSTIGAWRFNVDPDT
                     YGTPMVTASVDFVGTMALVVVAVALGLI"
     gene            2386..3570
                     /locus_tag="PKY13_11030"
     CDS             2386..3570
                     /locus_tag="PKY13_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80513.1"
                     /translation="MNLDFTPEEQAFREEARTWLAENLPTEERPPDGQPMREFDLAWQ
                     KTLFEGGWAGINWPKEFGGKGLNDIQQMIWYEEFARADPPFTLNNSCTFVGNNHGGPT
                     LIVSGTDEQKSFHLPKILGGEVVWCQGFSEPGAGSDLANLSCKAVIDGDELVVNGQKI
                     WTSYAHVADYQELLVRTDPTAPKHKGISWVICDMKTPGIEIREITTMSHVNDFCEVFY
                     DDVRIPLTNVVGQINDGWRVTMTTLSFERGTAFMADQVELASVVERLIEEARVRTGPD
                     GKRPAIADDEIARRLALARAEVAALKAMTIAGISRTQRTGVPGPEGSMTRLFHGELHQ
                     RVYQLALDIIGPEALKLTPVDGNGWTGPYLQSFAYTIGGGTTDIQRNIVGERVLGLPR
                     VK"
     gene            3596..>3777
                     /locus_tag="PKY13_11035"
     CDS             3596..>3777
                     /locus_tag="PKY13_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80514.1"
                     /translation="MDLLPTSEQEEIVGSIASVIAGNHTLGEPLDDRLWDAAVAQGWL
                     GLGLEEAFGGVGYSLA"
     assembly_gap    3778..3877
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3878..4138
                     /locus_tag="PKY13_11040"
     CDS             <3878..4138
                     /locus_tag="PKY13_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80515.1"
                     /translation="MCQVYYAALALRDGRDDAQFHAHAARVVAARAAIDNAQINVQNH
                     GGIGFTWEHTAHRYVTRSRVYGSALGTTHGHLGETLAASAPH"
     gene            complement(4154..5371)
                     /locus_tag="PKY13_11045"
     CDS             complement(4154..5371)
                     /locus_tag="PKY13_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT80516.1"
                     /translation="MDFDDSPEEAAFRQECRTWLAANAKLRTPGIAEVWRTLRPRDEG
                     EDDASLLVGKRWQALKADAGFAGIQWPVEYGGRGLSGHLAGVFKQEEARYDVPANSFQ
                     VGVDMVGPTLIAHGTPAQCQRHLDPIKRGDEVWCQLFSEPDAGSDLAGLSTRAVRDGD
                     EFVVNGQKVWTSGAQSSDWGILLVRTDPDQPKHRGITYLLVDMRTPGIEVRPLTQIDG
                     AVHFNEVFLSDVRVPVENVVGEIDGGWGVTMTTLVNERSAIGGGGMVQFAEILMLAQE
                     LGRTDEPVLRQELAKIYTYFQITRFLGYRVQTSISNGRQPGPESSVMKLHISRQYGEG
                     GDLYMSLLGAAGMLWLDDAPFDGFFQGLFLAQWAPRIGGGTDQIQRNIAGERVLGLPA
                     EPRVDKAVPFKDL"
     gene            complement(5485..6210)
                     /locus_tag="PKY13_11050"
     CDS             complement(5485..6210)
                     /locus_tag="PKY13_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80517.1"
                     /translation="MKRVLTVASSLVLATTCLWVSSAASQETTTTTTVEVTTTTEATT
                     TTAAPTTTVAEPEVTTTTEAPAETTTTVPEEPLVLPGQHVTAGQLAFDIRFTDSPAAN
                     CTKTLDLAGGQVNIGLDESGNIGGVYGLAPSGGGGNAGIFMIGLGAVPAGVAIVSVSD
                     GDCEFDAVGIGSFASDGGWARLNGVGAGLHPGYETPDYVNMFLVDAKVDASAAPAVLD
                     MNYVKSFLLRQRPGLEGYLPPEA"
BASE COUNT          985 a         2309 c         2152 g         1051 t
ORIGIN      
        1 acgcccaggt cttctacgcg atgaaggcca acccccatcc ggccgtgatc ggcctcctgg
       61 ccaccatggg gtgcggcatc gacgccgcgt cgaccaacga ggtcgacgtc gccgtcgccc
      121 acggcgtgcc acccgagcgc atctcgtacg gcaacacggt ccggcgggcg agcgaaatcg
      181 agcatgcccg cgacgccggt gtcgcgatgt tcgcggtcga cgacgaccac gagctcacca
      241 aggtgctcga ccacgccccc ggatcgacgg tcctggcccg cctgaacacc gagggaaccg
      301 gcgcggcgtg gccgctctcg cgcaagttcg gcaccgacgt gccgacggcg cagtcgatgc
      361 tcgtgcgggc gcagtcgcgg ggccacgggg tgggcgccac gttccacgtc ggctcgcagc
      421 agtccgaccc ctcctcctgg gacgacgcac tggtgcaggt cgcccagctg ctcgaggtgg
      481 tcgaggtgac gccggatacc ccgttcaccg tcaacctcgg aggtggcctg cccagtcgcc
      541 tcgccgaccc gactccggcc ttggccgagt tctccgaggt gatccacgcg gcgatcgccc
      601 gccacctcga cggtgccccc atctcgttcg ccatcgagcc cggccggttc ctcgtcggcg
      661 acgccggggt catcgaggcc gaggtcatcg gggtggtcga acgtccgggc caggcctgca
      721 gccgctgggt gttcatcgat gtcgggctgt tccaggggtt gtcggaggcg tcgggcgaat
      781 cgatccggta tccgctgtcg acgacggtcg gcggtccgac ctcgcccgcc gtcgtcgcgg
      841 ggccgacgtg cgacagcacc gacatcctct acgagcgccg gccggtcgag ctgccgctgg
      901 cgctgacgtc gggcgaccgc atccgtctcc acggcgccgg ggcctacacg gtctcgtgtt
      961 cgtccctggg cttcaacggg ttcgaacccc tccgcgtgga gatcgccccg tcctaggggc
     1021 ccgctgccaa cgccagtagg gtcggcgacc atggcacggt cgcgtcacgc cgccgggggc
     1081 ctgcgccccg accgcctgct gaggcgactg gtcgagctcc tgggcccgac cggcagcgcg
     1141 gcgcgccaga gcctcgtcgc cctcgggttc aactcggcga ccagcttcgc cgccggcgca
     1201 atgctcgtcg ggttcgagga cacgtggtcg cggctctcgc cgatgctcat cctcgttccg
     1261 gcggcgatcg gtctccgtgg caacgtgttc tcgacgctcg gcaaccggct ctcgacctcg
     1321 atccacaccg gtacgttccg cgtctccttc aaggccgatt ccgtgctcgg ccagaacctg
     1381 ctggcctcgt tcagcctgac gctcgtcatg tcggtgatcc tcgccgggtt cgccaaggtg
     1441 ctggccgtgg ccctcggcgt ggtccagcac gtgtcgttcc tcgaactcgc gatggtgtcg
     1501 gccctcggcg ggatcctcgg ttcggtggtg gtggcggcgg cgaccgtgct gctcacgatc
     1561 ggcgcagtgc gtttcgagtg ggacctcgac aacctcgtcg ctcccaccgt ctcgaccctc
     1621 ggcgacgtca tcaccatccc ggcgctgtac ctggcggcgc aactgatcgg acggggcagt
     1681 gtggcaaacg tgctcggcgg cgttgtggtc gtcatcacgg cggtggtcgc cgtgatgtcg
     1741 tggcgcaccg gcctcgagct cttcaaggag atcttccgtg agtcgctgcc ggtgctcgct
     1801 gcggcgctcg tgctgtcggc actcgccggg ctcgtcctgc agaagcagca gaacctcctc
     1861 cacgtgctcc cggcgctcgg catcctccag ccggcgttcg tgtcgtcggc cggcgcgctc
     1921 gggggcatcc tgtgcggacg catcgccacc aacctgcaca tcggttcggt cgaaccgacg
     1981 ctggctcccg gggccgaggc gagacgcgac ttctcgctcg tgttcggtct ggcggtgcca
     2041 ctgctcctgt tcaacgcgct gggcgcgtgg ctcgtctcgc tcctgacggg gtcgagcggc
     2101 gcccccgggt tcggatggct cctgtccacc agcctcctcg ccgccatcgt cacgatggcg
     2161 ttcgtggccg ccctcgcgta ctactccacc atcggggcct ggcggttcaa cgtcgacccc
     2221 gacacctacg gcaccccgat ggtgacggcg tcggtcgact tcgtgggcac gatggcgctc
     2281 gttgtcgtcg ccgtggcact cggcctgatc tgagcggctg gcgagtcgga ctggtcagcc
     2341 ctccgatggg ccgatctgac acaccgtcag atactctcgg catcgatgaa tctcgacttc
     2401 acccccgagg agcaggcatt ccgtgaggag gcccgcacct ggctcgcgga gaacctgccg
     2461 accgaggaac gtccgcccga cggccaaccg atgcgcgagt tcgaccttgc gtggcagaag
     2521 accctcttcg aagggggatg ggccggcatc aactggccga aggagttcgg tggcaagggc
     2581 ctcaacgaca tccagcagat gatctggtac gaggagttcg cccgcgccga cccgccattc
     2641 acgctcaaca actcgtgcac gttcgtgggc aacaaccacg gcgggccgac actcatcgtg
     2701 agcggcaccg acgaacagaa gtcgttccac ctgccgaaga tcctcggcgg cgaggtcgtc
     2761 tggtgccagg ggttctcaga gcccggcgcc ggttccgatc tcgccaacct cagctgcaag
     2821 gccgtcatcg acggcgacga gctcgtggtc aacggtcaga agatctggac gtcgtacgcc
     2881 cacgtcgccg actaccagga gctcctcgtc cgcaccgacc ccaccgcccc caagcacaag
     2941 ggcatcagct gggtcatctg cgacatgaag acgcctggta tcgagatccg tgagatcacg
     3001 acgatgagcc acgtcaacga cttctgcgag gtcttctacg acgacgtgcg catccccctg
     3061 acgaatgtgg tcggccagat caacgacggc tggcgggtca cgatgaccac gctcagcttc
     3121 gagcgcggta ccgcgttcat ggccgaccag gtcgagttgg cgtcggtcgt cgagcgcctc
     3181 atcgaggagg cccgggtccg caccggtccc gacggcaagc gcccggcgat cgccgacgac
     3241 gagatcgccc gccgcctcgc cctcgcccgg gccgaggtgg cggcgctcaa ggccatgacg
     3301 atcgccggga tcagtcggac ccagcgcacc ggcgtgcccg ggcccgaagg ttcgatgacc
     3361 cgcctcttcc acggcgaact ccaccagcgc gtgtaccagc tggccctcga catcatcggc
     3421 cccgaggccc tgaagctcac ccccgtcgac ggcaacggct ggaccggccc ctatctccag
     3481 agcttcgcct acacgatcgg tggtggcacc accgacatcc aacgcaacat cgtgggcgag
     3541 cgcgtgctcg gcctgccccg ggtcaagtga cccgcgatcg acaggactga ctgacatgga
     3601 tctactcccc acttcggaac aggaagagat cgtcggctcg atcgcctcgg tgatcgccgg
     3661 caaccacacg ctcggcgaac cactcgacga ccgcctctgg gacgccgcgg tggcccaggg
     3721 ctggctcggg ctcgggctcg aggaggcgtt cggcggtgtc gggtactcgc tcgccgannn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg tgccaggtgt actacgcggc
     3901 gctcgcccta cgcgacggac gcgacgacgc ccagttccat gcccacgccg ctcgcgtggt
     3961 cgccgctcgg gccgcgatcg acaacgccca gatcaacgtg cagaaccacg gtggcatcgg
     4021 cttcacctgg gaacacaccg cacatcgcta cgtcacccgg tcccgggtgt acggctcggc
     4081 cctgggcacg acccatggcc acctcggcga gacgcttgcc gcctccgcac ctcactgagc
     4141 ccgacccgtc agctcacaga tctttgaagg gcactgcctt gtcgacgcgg ggttcggcgg
     4201 gcaggccgag cacacgctca ccggcgatgt tgcgctggat ctgatcggtg ccgccgccaa
     4261 tgcgcggcgc ccactgggcg aggaacaagc cctggaagaa cccgtcgaac ggggcgtcgt
     4321 cgagccacag catgcctgcc gcgccgagca acgacatgta cagatcgccg ccctcgccgt
     4381 actgccggct gatgtgcagc ttcatgaccg agctctccgg accgggctgg cgaccgttcg
     4441 agatcgacgt ctgcacccgg tagcccagga accgcgtgat ctggaagtac gtgtagatct
     4501 tggcgagctc ctgacggagg accggctcgt cggtgcgacc cagctcctgg gccagcatca
     4561 ggatctcggc gaactgcacc atgccgccgc caccgatcgc actgcgttcg ttgacgagcg
     4621 tcgtcatcgt gacgccccaa ccgccgtcga tctcgcccac gacgttctcc accgggacgc
     4681 gcacgtcgct caggaacacc tcgttgaagt gcactgctcc gtcgatctgc gtgagcgggc
     4741 gcacctcgat gccgggcgtg cgcatgtcga cgagcaggta ggtgatgccc cggtgcttcg
     4801 gctgatcggg gtcggttcgg acgaggagga tgccccagtc cgacgactgg gcacccgacg
     4861 tccacacctt ctggccgttg accacgaact cgtccccgtc gcggaccgcg cgggtcgaga
     4921 gccccgcgag gtcggagccg gcgtcgggct cgctgaagag ctggcaccac acctcgtcgc
     4981 cccgcttgat cgggtcgagg tggcgttggc actgtgccgg cgtgccgtgg gcgatcagcg
     5041 tcggaccgac catgtcgacg ccgacctgga aggagttggc cggcacgtcg taccgcgcct
     5101 cctcctgctt gaagacgccg gcgaggtgac ccgacaggcc acgccccccg tattcgaccg
     5161 gccactggat accggcgaac ccggcgtcgg ccttgagcgc ctgccagcgt ttgccgacca
     5221 gcaggctggc gtcgtcctcg ccctcgtcac gaggccgcag ggtgcgccac acctcggcga
     5281 tacccggggt gcgcaacttg gcgttggccg ccaaccaggt gcggcactcc tggcggaacg
     5341 ccgcttcctc ggggctgtcg tcgaaatcca taccgggggg actgtacgca ggccacggcc
     5401 cgacaggcca acgccgaggc catccgccga tcggcgggaa tgggccgagg gacgactcgg
     5461 aggaccggca gcgccggacc ccgatcaggc ctccggcggg aggtacccct cgagaccggg
     5521 acgctggcgg agcaggaagc tcttgacgta gttcatgtcg aggaccgcag gggccgccga
     5581 ggcgtcgacc ttggcgtcga ccaggaacat gttgacgtag tcgggggtct cgtacccggg
     5641 gtgcagaccg gcgccgacac cgttgaggcg ggcccagccg ccgtcggagg cgaacgagcc
     5701 gatgcccacg gcgtcgaact cgcagtcgcc gtcgctcacg ctgacgatgg ccacaccggc
     5761 gggcacggcg ccgaggccga tcatgaagat gccggcgtta ccgccgccac ccgagggggc
     5821 gaggccgtac acgccaccga tgttgccgga ctcgtcgagg ccgatgttga cctgaccgcc
     5881 ggcgagatcg agcgtcttgg tgcagttggc tgcgggcgag tcggtgaagc ggatgtcgaa
     5941 ggcgagttga ccggcggtga cgtgctggcc ggggagcacg agcggctcct cggggaccgt
     6001 ggtcgtggtc tcggccggcg cttcggtcgt ggtggtcacc tcgggctcgg cgacggtggt
     6061 cgtcggagcg gcggtggtcg tggtcgcctc ggtcgtggtg gtgacctcga ccgtcgtggt
     6121 ggtcgtggtc tcctgcgacg ccgcgctgct gacccacagg caggtggtgg ccagcaccaa
     6181 tgaacttgcc accgtcagta cacgcttcac cgtcgcacct ctctcgtctc ttgcccgccg
     6241 tcgcccgcga gctgcaccag agtcgtcccc agacgccctg tcacagtaac cggggacccg
     6301 gggtccaaca aggcgtctgg gacattcacc tgcattcgat gcatttccgg gtccttcatc
     6361 ctcagtccct acccatcgac ggtgccgcct aacctcttga acgttctggc cgaacggtcg
     6421 gagacagggg gaccacatgt ccgaaccgga ccttctcgag ctgtcggagc gaatcatctc
     6481 cggagcgatg tcgatcgacg agcaccatcc cctgcagatg gcgagcaacg cccgtctgca
     6541 gccgatcacc gacaacatcg cgttcatcga gtcgttcgcc aacgtcgtcg cgacgcg
//
LOCUS       DAOSOA010000176         6588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11771_length_6588_cov_4.516149, whole genome shotgun sequence.
ACCESSION   DAOSOA010000176 DAOSOA010000000
VERSION     DAOSOA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11771_length_6588_cov_4.516149"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2556)
                     /locus_tag="PKY13_11055"
     CDS             complement(<1..2556)
                     /locus_tag="PKY13_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80518.1"
                     /translation="MNSAMRQVRPRHLVVCVLLALPLAVFFAPTLIAGNAPGAFDLSY
                     QSSPWRDEVTRPLDAQSPVQIDQAEQLPWVDGMWESLREGRIPMWSPAVGGGTSLGTN
                     PVFTTYSVFTIVGQLVGSATSLAVGLAVRILLATAVAELFGYLFLRRLKLSVGASVFG
                     TVAYVFSGAAVAFETRNAIPLLLPVVLWCTDRLAERVTPGRIVALAGTVLALWLEGFP
                     AMLVHALVVAALWWAVRVAIDNGLVFWRRPALGQWVRQAAAFGGSVGLGFVLSAVSAV
                     PFALQLGYNDVFAARSDEATPLGRISAWWLLDERALGVPDRGPWYIGLNPFEGVSAVG
                     TLVMVLALAGVLVSAFGRRGADRRQRDAVRNTWAVLVAVLAIAVFMGGPLLDALYALP
                     GFSGNPFSRIRYVIAFGLAVLAAIGIDEVEGWWRERSGEPVTRRWPHVVVALGFVACL
                     GLPFVASLGEYLDVLRGVRDLAVTTWRSELIVLGVGVAVLGVLVVTTRRLPERWTVGR
                     SLALGGVAAAIAFTQVGLQITRLTPQVDRAFYYPETNGYDALRAVAGHDLRFIGSGMG
                     TFAPNTAMTNDVVDARSHSFRDPQWKTLILSVFPDALGPDPLKINVDFHGNVDWDSPV
                     FDDLAIGVVAMSSSEVPLGDRHDVPEAQSWIPVRGVSAVDADAPDGGYVGLEFRLRTS
                     GNCGQGDVVVTATPDGGPAAVSRRPLGDARAAGVADAPLPFAVPMDDAVRSEMSVRLD
                     GAPADCVLEVGATDASETVPAVAPITRPVDAPWEMASADGGWLYTRSSAHPLVRTVAD
                     WTPLADRSDALEAAVDPSRTSAAPMPVATATDVPDGATAGTVESWSADDHGISA"
     gene            complement(2650..3828)
                     /gene="moeB"
                     /locus_tag="PKY13_11060"
     CDS             complement(2650..3828)
                     /gene="moeB"
                     /locus_tag="PKY13_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HOT80519.1"
                     /translation="MPSFRELLTATKSEIAEVTTAEAEELRADGAIVLDVREADEFEQ
                     GAIPGAVFIPRGHLEGQIENRIADKDAKIVVHCAGGVRSAFAAKTLNELGYTDVVSMA
                     GGFNKWKDEGRDWRTPQVLTPEQRNRYQRHLLVPEVGEEGQQKLLDAKVLLLGAGGLG
                     SPAALYLAAAGVGTIGIIDMDVVDASNLQRQILHNLDRIGDRKVDSAKKTLTLLNPDV
                     DVITYDRRLDASNIEEIITGYDLIVDGADNFPVRYMLNDASVKLGIPVVHGSIFRFEG
                     QVTVFDPKNGPTYRDMLPEPPPAEMAPSCAEAGVLGALPGIIGTIQAVEAIKLILGAG
                     SPLIGRYLTFDALDMEFREYKIRKDPNNQVTWENRDQIQVVELEGLCQPAPLQAPANA
                     "
     gene            4009..4596
                     /locus_tag="PKY13_11065"
     CDS             4009..4596
                     /locus_tag="PKY13_11065"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT80520.1"
                     /translation="MSERKHQPIYTRKGDDGTTGLYFGGRVSKDSVRPAAYGDVDELQ
                     SIMGLARSHPHDDQRLDEIVISLQRDLWVVMADLATGADNRNKLTDGSTKVTTTMVER
                     LETLIDEVSTWFTPPTEFVVPGGNVVAATIDVARTVARRSERSAVAIGDTGSLATTYL
                     NRLSDLLWTLARWVEGTSLTVRSVPTNENVPQESP"
     gene            4593..6098
                     /locus_tag="PKY13_11070"
     CDS             4593..6098
                     /locus_tag="PKY13_11070"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOT80521.1"
                     /translation="MTTLKLARTASARAAVVATLVPSDQLRSSGVDKRLLTRLRFEGK
                     PGQTATVTGESPAVHVLVGVGPSLEVDTTGVRKAVAAAVRALSSDKTVVIDLSLIDLS
                     LSDADLTADDLAQAAAEGAGLAAYRFDRYKAENDDPRLETATLVVAGGGKDGFARGLA
                     IVDAVNFARDLVNEPGGTLTPTRFADLAAERAGDAGLTVEVLGPDEIEEGALGGLLSV
                     NRGSEQEARFVKLTYTPSDEAAAARDSVALVGKGITFDSGGLSIKPADGMMTMKCDMG
                     GAAAVIATMCALPALRVGVPVTSYTPMTDNMTGGDATRPGDVMTARNGKTVEILNTDA
                     EGRLVLADALSLASEDGHSAIVDLATLTGACMVALGDQIAGLMGNDDELIGEIEAAAE
                     TSGEKFWHLPLPPEYRKMLDSPIADMKNIGSRYGGALTAGLFLQEFVSDDISWAHLDI
                     AGPAFTEAADGEIPRGGTGFAVRTLLAWLEARAAGAELDEEADASDEPSAD"
     gene            complement(6150..>6588)
                     /locus_tag="PKY13_11075"
     CDS             complement(6150..>6588)
                     /locus_tag="PKY13_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80522.1"
                     /translation="IYADTDRSLTNGPGALLDGGFPVSAGVNTYTWDGHGVPAGAYYI
                     HVKIRRGGRDVGATSTGQVAVGTPGAGPAPDARGDLAGQAAPPSSGPTQAQLIAFFNY
                     ILTIKFFCSVARARNILAAGAAPVCNQLLGPWVRPKAKAKRRR"
BASE COUNT         1102 a         2363 c         2224 g          899 t
ORIGIN      
        1 ggcggagatg ccgtggtcgt cggcggacca cgattcaaca gtgcccgcgg tcgccccgtc
       61 gggaacgtcg gtcgcggtcg cgaccggcat cggcgcggcc gacgtacgcg acggatcgac
      121 ggccgcctcg agagcgtcgg accggtcggc gagcggggtc cagtcggcga ccgtgcggac
      181 gagcgggtgc gcggaggagc gggtgtagag ccatccgccg tcggcggagg ccatctccca
      241 gggggcatcg acgggacggg tgatcggcgc cacagccggc accgtctccg aggcgtcggt
      301 ggccccgacc tcgagcacgc agtccgcggg ggcgccgtcg aggcggaccg acatctcgga
      361 acgaactgcg tcgtccatcg gcacggcgaa cggcaacggt gcatcggcga caccggccgc
      421 acgggcgtcg cccaacggac ggcgggacac cgccgccgga ccgccatcgg gagttgcggt
      481 caccacgacg tcgccctgcc cgcagttgcc cgacgtgcgc agccggaatt cgaggccgac
      541 gtacccaccg tccggcgcgt cggcatcgac ggccgagaca ccccggaccg gaatccagct
      601 ctgcgcctca gggacgtcat gacgatcgcc cagcggcacc tcgctcgacg acatcgcgac
      661 gacgccgatg gcgaggtcgt cgaagaccgg cgaatcccag tcgacgttgc cgtggaagtc
      721 gacgttgatc ttgagcggat cgggaccgag cgcgtcgggg aacaccgaca ggatcagcgt
      781 cttccactgc ggatcgcgga acgagtggct gcgcgcatcg acgacgtcgt tcgtcatcgc
      841 ggtgttcggc gcgaacgtgc ccatgccgct gccgatgaac cggaggtcgt gaccggcgac
      901 ggcccgtaac gcgtcgtagc cgttggtctc ggggtagtag aacgcgcggt cgacctgcgg
      961 agtgaggcgg gtgatctgca ggccgacctg ggtgaacgcg atcgccgcag ccacaccgcc
     1021 caacgccaga ctgcggccga cggtccagcg ctccggcagt cgccgcgtcg tgacgacgag
     1081 caccccgagc accgcgacgc cgacgccgag cacgatcagc tcgctccgcc aggtggtgac
     1141 ggccaggtcg cggacccctc gcagcacatc gaggtactcg ccgagcgacg ccacgaacgg
     1201 gagtccgaga cacgcgacga agccgagggc gaccacgacg tggggccatc ggcgcgtcac
     1261 cggttcgcca ctccgctcac gccaccagcc ctcgacctcg tcgatgccga tggcagcgag
     1321 caccgccagg ccgaacgcga tcacgtaccg gatgcgcgag aacggattgc cggagaaccc
     1381 cggcagggcg tacagcgcgt cgagcagcgg cccacccatg aacacggcga tcgcaagcac
     1441 ggcgaccagc acagcccacg tgttccgcac agcgtcgcgt tgtcgacgat cggcgccccg
     1501 acgtccgaat gcgctcacga gcacccctgc gagggccagg accatcacca gcgtgccgac
     1561 ggccgacacc ccttcgaacg gattgagccc gatgtaccac ggaccccggt ctggcacacc
     1621 gagcgcccgc tcgtcgagga gccaccaggc actgatgcga ccgagcgggg tcgcctcgtc
     1681 ggaacgagct gcgaacacgt cgttgtaccc gagctggagg gcgaacggca ccgcgctgac
     1741 ggcgctcaaa acaaacccga gcccgaccga tccgccgaac gccgcagcct gacgaaccca
     1801 ctgcccgagc gccggccgcc gccagaacac gagcccgttg tcgatcgcca ctcggaccgc
     1861 ccaccacagc gccgcgacca cgagcgcatg gacgagcatc gcggggaagc cctcgagcca
     1921 cagcgccagc accgtccccg ccagcgccac gatcctgccg ggcgtgaccc gctcggcgag
     1981 acgatcggtg caccacagca caacagggag gagcaacggg atggcgttgc gcgtctcgaa
     2041 cgcgaccgcc gccccactga acacgtacgc gaccgtgccg aagacgctcg cacccacgct
     2101 caacttcaga cggcgcagga acaggtagcc aaacagctcg gcgacggccg tggccagcag
     2161 gatacgcacg gcgaggccga cggcgaggct cgtggcactg ccgaccagct ggccgacaat
     2221 cgtgaacacc gagtaggtgg tgaacaccgg gttcgtaccc agcgaggtgc cgccaccgac
     2281 cgccgggctc cacatcggga tccgcccctc gcggagcgat tcccacatgc cgtcgaccca
     2341 gggcagctgc tcggcctggt cgatctggac gggcgactgc gcatcgagcg gtcgggtcac
     2401 ctcgtcacgc cacggcgagc tctggtagct gaggtcgaac gccccgggag cgttcccggc
     2461 aatcagggtc ggcgcgaaga acaccgccaa cggcagcgcc agaaggacgc acaccaccag
     2521 gtgccgggga cggacctgcc gcatcgccga attcatcggc agcgattctg tcagtcgcgc
     2581 tccgtcgcga ccgaaatgcg tcgtgaggtg cacgcgcagg tgtgcacctc gcgaggcatt
     2641 cggatcttgc tacgcgttcg ccggcgcctg cagcggcgcc ggctggcaga ggccctcgag
     2701 ctccacgacc tggatctggt cccggttctc ccaggtgacc tggttgttcg ggtccttgcg
     2761 gatcttgtac tcgcggaact ccatgtcgag cgcgtcgaac gtcaggtagc gcccgatgag
     2821 cggcgagccg gcgccgagga tcagcttgat cgcctcgacg gcctggatgg tgccgatgat
     2881 gcccggcagc gcacccaaca cgccggcctc ggcgcagctc ggcgccatct cggcgggcgg
     2941 cggttcggga agcatgtcgc ggtaggtggg gccattcttc ggatcgaaca ccgtgacctg
     3001 gccctcgaag cggaagatcg acccgtgcac cacggggatg ccgagcttca ccgacgcatc
     3061 gttgagcatg taccgcaccg ggaagttgtc ggcgccgtcg acgatgaggt cgtagccggt
     3121 gatgatctcc tcgatgttgc tggcatcgag acgacggtcg taggtgatga cgtcgacgtc
     3181 ggggttgagg agcgtgagcg tcttcttggc cgagtcgacc ttgcggtcgc cgatgcggtc
     3241 gaggttgtgg aggatctgac gctgcaggtt ggaggcgtcc accacgtcca tgtcgatgat
     3301 gccgatcgtg ccgacaccgg ccgccgccag gtagagcgct gcaggcgaac cgagcccgcc
     3361 ggccccgagc agcaggacct tggcgtcgag gagcttctgc tgaccttcct cgcccacctc
     3421 gggaacgagg agatggcgct ggtagcggtt gcgctgctcg ggcgtgagca cctgcggcgt
     3481 ccgccagtcg cggccctcgt ccttccactt gttgaagccg ccggccatcg acacgacgtc
     3541 ggtgtagccg agctcgttca gcgtcttcgc agcgaaggcg gaacggaccc cgccggcgca
     3601 gtggacgacg atcttggcgt ccttgtcggc gatgcggttc tcgatctggc cctcgaggtg
     3661 gccgcggggg atgaagaccg ccccgggaat ggcgccctgc tcgaactcgt cggcctctcg
     3721 cacgtcgagc acgatcgcgc cgtcggcacg cagctcctcg gcctcggccg tggtgacctc
     3781 ggcgatctcg gacttggtgg cggtgagcaa ctcacggaat gacggcatga cgaactctcg
     3841 ctatctctgg acctgatgaa aaccctactg aattggtcaa cattgtggaa agtggatgca
     3901 ttccgcacgg cctgtcgacg tcaccgatag ggtcgaacga gcgccgtagg cgctccaatc
     3961 aggacggatc gttgtcggcg aggccgacgg cccatcggag ccactgacgt gagtgagcga
     4021 aagcaccaac ccatctacac gcggaagggc gacgacggca cgaccggcct gtacttcggt
     4081 ggacgggtgt cgaaggactc cgtgcgtccc gccgcctacg gcgacgtcga cgagctccaa
     4141 tcgatcatgg gcctggcccg ctcccaccct cacgacgacc agcgcctcga cgagatcgtc
     4201 atctccctgc agcgcgacct gtgggtcgtg atggccgatc tcgccaccgg cgccgacaac
     4261 cggaacaagc tgaccgatgg cagcaccaag gtcacgacca ccatggtcga gcggctcgaa
     4321 acgctcatcg acgaggtcag cacctggttc actcccccga cggagttcgt ggtgcccggc
     4381 ggcaacgtgg tcgcggccac gatcgacgtc gcccgcaccg tcgcccgtcg ctccgaacgg
     4441 agcgccgtgg ccatcggtga caccggctcg ctcgccacga cctacctcaa ccgcctctcc
     4501 gacctgctgt ggacgctcgc ccgttgggtc gagggcacgt cgctcaccgt ccgctccgtc
     4561 cccaccaacg agaacgtccc ccaggagtcc ccatgaccac cctcaagctc gcccgtactg
     4621 ccagcgcccg cgccgcggtc gtcgccactc tcgtgccgtc cgaccagctc cgatcctcgg
     4681 gcgtcgacaa gcgcctcctc acccgcctcc ggttcgaggg caagcccggc cagacggcga
     4741 cggtcaccgg cgagtcaccc gctgtgcacg tgctcgtcgg cgtcggcccc tcgctcgagg
     4801 tcgacacgac cggcgtccgc aaggccgtcg cagccgccgt ccgggcgttg tcctccgaca
     4861 agacggtggt catcgacctg tccctcatcg acctctcgct gagcgacgcc gacctcaccg
     4921 ccgacgatct cgcccaggca gcagccgagg gtgccgggct cgccgcctac cgcttcgacc
     4981 ggtacaaggc cgagaacgac gacccccgcc tcgagacggc gacgctcgtg gtcgccggcg
     5041 ggggcaagga cggcttcgct cgcgggctcg cgatcgtcga cgccgtgaac ttcgcccggg
     5101 acctcgtcaa cgagcccggc ggcacgctca cgcccacccg tttcgccgac ctcgccgccg
     5161 aacgcgccgg cgacgccggc ctcaccgtcg aggtgctcgg gcccgacgag atcgaggagg
     5221 gggcgctcgg cgggctgctc tcggtcaacc gcggctcgga gcaggaagcc cgcttcgtga
     5281 agctgaccta caccccgtcc gacgaggccg ccgccgcccg cgactcggtc gccctcgtgg
     5341 gcaagggcat cacgttcgac tccggcggac tcagcatcaa gcccgccgac ggcatgatga
     5401 cgatgaagtg cgacatgggc ggtgccgccg cggtgatcgc cacgatgtgt gccctgccgg
     5461 ccctgcgcgt cggcgtgccc gtcaccagct acacgccgat gaccgacaac atgaccggcg
     5521 gcgacgccac ccgccccggc gacgtcatga cggcgcgcaa cggcaagacc gtcgagatcc
     5581 tcaacaccga cgccgagggc cgcctggtgc tcgccgacgc gctgtcactg gcgtcggagg
     5641 acggccactc ggccatcgtc gacctcgcca cgctgaccgg cgcctgcatg gttgccctgg
     5701 gtgaccagat cgccgggctc atgggcaacg acgacgagct gatcggcgag atcgaggcgg
     5761 ctgcggagac atcgggcgag aagttctggc acctgccgct gccccccgag taccgcaaga
     5821 tgctcgactc ccccatcgcc gacatgaaga acatcggcag ccgctacggc ggggcgctca
     5881 ctgccggact gttcctccag gagttcgtga gcgacgacat ctcgtgggcc cacctcgaca
     5941 tcgccggccc ggccttcacc gaggcggccg acggcgagat cccccgcggc ggcaccggtt
     6001 tcgcagtacg aaccctgctc gcctggctcg aggcccgcgc tgccggggcc gagctcgacg
     6061 aggaggccga cgcgtccgac gagccgagcg ccgactgacg cgatcggagg gtcagcgggg
     6121 cgtcgatgct ccgcggaccc ccgatcccgt caacggcgcc gcttcgcctt cgccttgggg
     6181 cggacccacg ggccgagcag ctggttgcac accggcgccg cgccggcagc gaggatgttg
     6241 cgagcccggg cgacactgca gaagaacttg atcgtcagga tgtagttgaa gaacgcgatc
     6301 agctgcgcct gcgtcggccc ggacgacggc ggtgccgcct ggccggcgag atcaccccgg
     6361 gcgtcgggcg cgggcccagc gcccggagtc ccgacggcga cctgcccggt cgaggtggct
     6421 ccgacgtcgc gaccgccacg gcggatcttg acgtggatgt agtaggcacc cgccggcacc
     6481 ccgtgtccgt cccatgtgta ggtgttgacc ccggcggaca ccgggaagcc cccgtcgagg
     6541 agtgctccag gtccgttggt gaggctgcga tcggtgtccg cgtagatg
//
LOCUS       DAOSOA010000177         6569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11814_length_6569_cov_5.743783, whole genome shotgun sequence.
ACCESSION   DAOSOA010000177 DAOSOA010000000
VERSION     DAOSOA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11814_length_6569_cov_5.743783"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PKY13_11080"
     CDS             <1..1371
                     /locus_tag="PKY13_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80523.1"
                     /translation="ELDPAAVDRLESIHDEIFELSGRTSRFGAGKQRRRLEHLRAEET
                     KLLEELGFDTWSSYIMGITSAGSDPARRRDYENAVRAYEQAEAELEHAANDPITVEVD
                     PEYNDLQRARADLVHLIEAFLGTEVGADPVPALRAVQVEVAAIAPPAALPRSQPAVPV
                     APLGDVDGDAGELGRALQDALVGTGANLPDRELSLIELTSLAAGWLETMEQLPQRISA
                     ATQQRDGIEEEISELAVELDALPDEATVVDAHQPVEPDPEPELGEPEETLELIEARAR
                     VAEGEARVRAHEEAVARIAEVEAEYERLLQRSRELQKSLAEREPRVASLLEQRVAARN
                     RLRRAENNRSAVESARWDNRHQTSEESSRFLGGSAGAEAVEWYVLARLAQQRSVSFVG
                     SVPLVIDDAFANWPVEALGDVFTRLERMGEVIQIVYLTDDPDVGAWARSLGSDRALVL
                     DMRVAS"
     gene            1494..2672
                     /locus_tag="PKY13_11085"
     CDS             1494..2672
                     /locus_tag="PKY13_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT80524.1"
                     /translation="MEFRRITSLPPYVFTIIDSLKIEQRRAGKDIIDLGFGNPDLASP
                     DVAVDKLIEAAQNSRNHRYSSSRGIPKLREAIAGYYQRRFGVTLDPETEVINTIGAKE
                     GFSHLMWTLLQPGDAALVPSPSYPIHIYGPLLAGAELRELPMINGAEQFFDAMVDTWK
                     YSLPKPRVIVLSFPHNPTTTCVDLDFFQRVVDFAREKEVVVVHDNAYADLGFDGYQPP
                     SILQAEGAKECAVELYSMTKSFSMAGWRVAFMLGNPEVIAALAKLKSYLDYGTFQPIQ
                     IAATVAINEAPDFPAEQNAIYQVRRDTLCEGLNRIGWEIEPPKGTMFAWAPIPEPYRD
                     LGSIEFAKLLVTEAEVAVSPGVGFGPDGDGHVRFAMIENEQRTAQAIRNLRRALVKLG
                     "
     gene            2689..3819
                     /locus_tag="PKY13_11090"
     CDS             2689..3819
                     /locus_tag="PKY13_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80525.1"
                     /translation="MAHVVERIVAIVVGILLVCWVLDAAIRNFLLPRASRVRLTQWIS
                     RVVARVFNLFARPSQPYERRDKVLAMRPPFTLLAFQAVWLFIVFVGFVLIFWGAAEEV
                     SWRTAMNESGSALFTLGFATPESGVPFFVVFAEAVIGLTLTALLISYLPTIYAAFQKR
                     EFMVAKLAVRTGSSGAPWRALSVAHITGSLEWMDTSFWQEWEDWFIDIAESHTSLTIL
                     NFYRSPDPSNHWISAARTVLDMAALRIAAVDRPTTVGPQIAIRNGTIALRVLARHFRI
                     EHDPDPQPHHPISLTREHFDVACRMMEESGVPIVADRDAAWIAFAGWRVNYDTIIEQA
                     AVAFMAPPSPWDTIADAQPVNAALFPKAAGGAIVGAGDDSPL"
     gene            complement(3830..4444)
                     /locus_tag="PKY13_11095"
     CDS             complement(3830..4444)
                     /locus_tag="PKY13_11095"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT80526.1"
                     /translation="MPSDDTPRPEKRRLTPRGTQRRLQLIELATAKFAADGYHPTSVA
                     DIVDGLGVGKGVFYWYFDSKEMLFEEILRDAQKDLRRRQQRAVSDLPDPIERMEQGIR
                     AAVHWSFEHRDLAKLVDFAQTDERFAHLIRHGRSRLVGDAMPLIKEAMVEGLIPEGDP
                     EQLAYAILGVSTNLTAVYINEQGQDPDAIAQVAVDFCLRGIGAR"
     gene            4611..5258
                     /locus_tag="PKY13_11100"
     CDS             4611..5258
                     /locus_tag="PKY13_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT80527.1"
                     /translation="MALFVVRVWLPDRPGALGAVASRIGAVHADVVGIDILERGAGRA
                     VDELTIDIADARHVDLLVQEIAQVDGVDVEHVRPARSSFSDRAGESLEVAASIMEAAS
                     TEAALDVLVIGVRDLFDADWAAVIDPSAGRSIAVAGGAEMPSAEWLSAFIIGTGVGEG
                     AYAAVDELARTSLPDHGAELVVSRQHLPLREGERRIMATLARITDRRLEAAADVS"
     gene            complement(5283..>6569)
                     /gene="glpK"
                     /locus_tag="PKY13_11105"
     CDS             complement(5283..>6569)
                     /gene="glpK"
                     /locus_tag="PKY13_11105"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOT80528.1"
                     /translation="RSTGMPLHRAIVWQDRRTAARCDELVEAGHLPLVRSTTGLVLDP
                     YFSGSKVEWLRRHGGVDLGPDTAVGTIDSWILWNLTGGTAAAGAVHATDPSNASRTML
                     FDIGTGTWSDELCELFGVPRSVLPEVRPSAGRFGVTTDGFAGGAGIPVSGIAGDQQSA
                     LFGQACFEVGMTKNTYGTGSFVLMNIGDTLPVPTDGLLTTVAWTLPGSMLSPGESPDL
                     VTHYALEGAIFATGAAIQWLRDGLGIIADAAETGPLAESVESSGGVFLVPAFAGLGSP
                     YWDPYARGTIVGLTRGSGRAELVRATVESMAYQTRDVIDSMSAASGTPVKELRVDGGA
                     SVMPILLRLVAEQLGVPVVRPRLQETTALGAAYLAGLAEGVWTTVEEVSASWAVDERV
                     APPASQADGDRAAVDAGYAMWQRAVERSRSWATDEA"
BASE COUNT          987 a         2331 c         2226 g         1025 t
ORIGIN      
        1 gagctcgacc cggcggcggt cgaccgcctc gagtcgatcc acgacgagat cttcgagctc
       61 agcggccgga cgtcacgctt cggtgccggc aagcaacgtc gacgtctcga gcacctgcgg
      121 gccgaagaga cgaagctcct ggaggagctc ggcttcgaca cgtggagctc ctacatcatg
      181 ggcatcacgt cggcggggtc cgatcccgcc cgtcgccgtg actacgagaa cgccgtgcgt
      241 gcctacgaac aggccgaggc ggagctggag cacgctgcca acgatccgat caccgtcgag
      301 gtcgatccgg agtacaacga cctgcagcgt gcccgcgccg acctggtcca cctcatcgag
      361 gcgttcctcg gcaccgaggt cggggccgat cccgtgccgg cgctgcgcgc cgtccaggtc
      421 gaggtcgcag cgatcgcccc tcccgccgcc ctccctcgct cacagccagc agtccccgtt
      481 gccccgctcg gagacgtcga cggcgacgcc ggcgagctcg gtcgggcact ccaggatgcc
      541 ttggtcggca ccggggcgaa tctgcccgat cgcgagctct cgctgatcga actcacgtcg
      601 ctcgccgccg gatggctgga gaccatggag cagctgccgc agcgcatctc ggccgccacc
      661 cagcagcgcg acgggatcga ggaggagatc agcgagctgg ccgtcgaact cgacgccctc
      721 cctgacgagg ccaccgtcgt cgatgcgcac cagccggtcg aacccgaccc cgagccggaa
      781 ctcggcgagc ccgaggagac cctcgaactg atcgaggcgc gtgcccgggt tgccgagggc
      841 gaagcgcggg ttcgtgccca cgaggaggcc gtcgcccgca tcgccgaggt cgaagcggag
      901 tacgagcgcc tcctgcagcg gtcccgtgag ctccagaagt ccctcgccga acgcgagccc
      961 cgggtcgcat cgctgctcga gcagcgcgtc gccgcccgca accggctccg tcgtgccgag
     1021 aacaaccgca gcgcggtcga gtcggcccgg tgggacaacc gccaccagac gtcggaggaa
     1081 tcgtcccggt tcctcggcgg gtcggccggc gccgaggcgg tcgagtggta cgtgctggcc
     1141 cgcctggcgc agcagcggtc ggtgtccttc gtcggctcgg tgccgctcgt gatcgacgac
     1201 gccttcgcca actggcccgt cgaggcgctc ggcgacgtct tcacccgcct cgagcgcatg
     1261 ggcgaggtca tccagatcgt gtacctcacc gacgaccccg acgtcggtgc gtgggcccgc
     1321 agcctcggct ccgaccgggc cctcgtgctc gacatgcgag tggcctccta agtcgcccgc
     1381 ctcaccggtt ctgtcctgca gaaccgcccc tccaggggcg gattcgcagg acagtacggg
     1441 tgtcacacgc aattgatccg ggccctgcgc gggggaccgg tagtgtcgac ggcatggagt
     1501 tccggcgcat cacgtcccta ccgccgtatg tcttcaccat catcgactcg ctcaagatcg
     1561 agcagcgacg cgctggcaaa gacatcatcg acctggggtt cggcaacccc gacctggcgt
     1621 cccccgacgt cgcggtggac aagctgatcg aggcggcgca gaacagccgg aaccaccgct
     1681 actcgtcgag ccgtggcatc cccaagctcc gcgaggcgat cgccgggtac taccagcgtc
     1741 gtttcggcgt cacgctcgat cccgagaccg aggtcatcaa cacgatcggc gccaaggagg
     1801 ggttcagcca cctcatgtgg acgctcctgc agcccggcga tgccgcgctc gtgccgtccc
     1861 cgagctaccc gatccacatc tacgggccgc tcctggccgg ggccgagctg cgcgagctcc
     1921 cgatgatcaa cggcgccgag cagttcttcg acgccatggt cgacacgtgg aagtacagcc
     1981 tccccaagcc gcgggtgatc gtgctgtcgt tcccgcacaa ccccacgacc acgtgcgtcg
     2041 acctcgactt cttccagcgg gtcgtcgact tcgcgcgcga gaaggaggtc gtggtcgtcc
     2101 acgacaacgc ctacgccgac ctgggcttcg acggctacca gccgccgtcg atcctgcagg
     2161 ccgagggcgc gaaggagtgc gccgtcgagc tgtactcgat gaccaagtcg ttctcgatgg
     2221 ccggttggcg tgtcgcgttc atgttgggca accccgaggt catcgctgcg ctcgccaagc
     2281 tcaagagcta cctcgactac ggcacgttcc agccgatcca gatcgcggcg actgtggcca
     2341 tcaacgaggc acccgacttc ccggccgagc agaacgccat ctaccaggtc cggcgcgaca
     2401 ccctctgcga gggtctcaac cgcatcggat gggagatcga gcccccgaag ggaacgatgt
     2461 tcgcgtgggc gccgattccc gagccctacc gcgacctcgg ttcgatcgag ttcgcgaagc
     2521 tgctcgtgac cgaggccgag gttgcagttt cgcccggcgt ggggttcggc cccgacggcg
     2581 acggccacgt ccggttcgcc atgatcgaga acgagcagcg cacggcccag gccatccgca
     2641 acctgcgtcg tgcgctcgtg aaactgggct gatcggcacc gtcggccgat ggcgcacgtc
     2701 gtcgaacgca tcgtcgccat cgtcgtcggc atcctgctgg tgtgctgggt gctcgacgcc
     2761 gccatccgca acttcctgct cccacgggcg agccgggtgc ggctgacgca gtggatcagt
     2821 cgggtcgtcg ctcgggtgtt caacctgttc gcccggccct cgcagcccta cgagcggcgc
     2881 gacaaggtcc tggccatgcg gccacccttc acactgctgg cgttccaggc cgtgtggctg
     2941 ttcatcgtgt tcgtgggctt cgtgctgatc ttctggggcg cggccgagga ggtgagctgg
     3001 cggacggcga tgaacgagag cggctcggcc ctgttcaccc tcgggttcgc cacgcccgag
     3061 agcggggtgc cgttcttcgt ggtcttcgcc gaggccgtca tcggcctgac gctcaccgcg
     3121 ctgctcatct cgtatctgcc gacgatctac gcggcgttcc agaagcgcga gttcatggtt
     3181 gcgaagttgg ccgtccgcac cggcagctcc ggcgcgccgt ggcgggcact ctcggtggcg
     3241 cacatcaccg ggtcgctcga gtggatggac acctcgttct ggcaggagtg ggaggactgg
     3301 ttcatcgaca tcgcggagtc gcacacgtcg ctgacgatcc tcaacttcta ccggtcgccg
     3361 gacccgtcga accactggat ctcggcggca cgcacggtgc tcgacatggc agcgctgcgg
     3421 attgccgcgg tcgatcggcc gaccaccgtc gggccgcaga tcgcgatccg caacggcacg
     3481 atcgccctac gcgtcctggc ccgccacttc cgcatcgaac acgatccgga tcctcagccc
     3541 caccacccga tctcgctcac ccgtgagcac ttcgacgtgg cctgccggat gatggaggag
     3601 tcgggcgtgc cgatcgtcgc cgaccgcgat gcggcgtgga tcgcgttcgc cggctggcgg
     3661 gtcaactacg acacgatcat cgagcaggcc gcggtggcgt tcatggcgcc gccgtcgccg
     3721 tgggacacca tcgccgatgc gcaaccggtg aatgcagcgt tgttccccaa ggctgcaggc
     3781 ggcgcgatcg tcggcgccgg cgacgactcg ccgctctgat cggcagcgct cagcgggcgc
     3841 cgatgccgcg gagacagaag tcgacggcca cctgggcgat cgcgtcggga tcctgaccct
     3901 gttcgttgat gtacaccgcg gtgaggttgg tgctcacgcc gaggatcgcg tacgccagct
     3961 gctcgggatc gccctcaggg atgaggccct cgaccatggc ctctttgatg aggggcatgg
     4021 cgtcgcccac gagtcgggag cggccgtggc ggatcaggtg cgcgaaccgt tcgtcggtct
     4081 gggcgaagtc gacgagcttg gcgaggtcac gatgttcgaa cgaccagtgc accgcggccc
     4141 gaatgccttg ctccatccgc tcgatcgggt cgggaagatc ggacacagca cgttgctggc
     4201 gccgacgtag gtccttctgt gcgtcgcgca ggatctcctc gaagagcatc tccttcgagt
     4261 cgaagtacca gtagaagacg cccttgccca cgccgaggcc gtcgacgatg tcggccaccg
     4321 acgtcgggtg gtacccgtcg gcggcgaact tggcggtcgc cagctcgatg agctggaggc
     4381 ggcgctgcgt tccccgcggc gtcagccgac gcttctcggg acgcggcgtg tcgtcgctgg
     4441 gcatgcgccg aagctacgga cccttgaccg cgcggtcaag agagcgcagc gcagtcgcac
     4501 cgtgtggggt cgtgtgcgcg ggccacgaga cggcggcgac gcgctcgtgg ttcatcgtcc
     4561 gttgacatcg caccttcatt gcattcacag accccaacta cggtgtgtcg atggctttgt
     4621 tcgtcgttcg agtctggctg ccggatcgcc cgggggcgct cggagcagtc gcgtcgcgca
     4681 tcggtgccgt ccacgccgac gtcgtcggca tcgacatcct cgaacgcggc gccggtcgtg
     4741 cggtcgacga gctgacgatc gacatcgccg atgcgcgcca cgtcgatctg ctggtgcagg
     4801 agatcgccca ggtcgacggc gtcgacgtcg agcatgtccg tccggctcgc tcctccttct
     4861 cggatcgcgc cggcgagtcg ctcgaagtgg ctgcctcgat catggaggcc gcctccacgg
     4921 aggcggccct cgacgtgctg gtgatcgggg tgcgtgatct gttcgacgcc gactgggctg
     4981 cggtcatcga tccgtccgcc ggccgctcga tcgccgtcgc cggtggcgcc gagatgccct
     5041 cggcggaatg gctgtcggcg ttcatcatcg gcaccggcgt cggtgaaggg gcctacgccg
     5101 cggtcgacga gctggcccgg acgtcgctgc ccgaccacgg cgccgagctc gtcgtgtccc
     5161 gacagcacct gccgctgcgc gagggcgagc ggcgcatcat ggccaccctc gcccgcatca
     5221 ccgaccgtcg cctcgaggcg gccgccgacg tctcctgagc cgaacccggt gaggggcggg
     5281 cgtcaggcct cgtcggtggc ccaggagcgc gaacgttcga cggcgcgctg ccacatcgcg
     5341 tagcccgcat cgacggccgc ccggtcgcca tcggcctggc tcgccggcgg cgcgacccgc
     5401 tcgtcgacgg cccaactcgc cgatacctcc tcgaccgtcg tccacacacc ttcggccagt
     5461 ccggccaggt aggccgcgcc cagggcggtc gtctcctgaa ggcgaggtcg caccacgggc
     5521 accccgagct gctcggccac gagccggagg aggatcggca tcaccgacgc cccgccgtcg
     5581 actcgcaact ccttgaccgg cgtgccgctc gctgccgaca tggagtcgat gacgtcgcgg
     5641 gtctggtacg ccatcgattc gacggttgcc cgcaccagct cggcccgccc cgagccgcgg
     5701 gtcagcccga cgatggtgcc gcgggcgtac gggtcccagt acggcgagcc gaggccggcg
     5761 aacgccggga ccaggaacac cccaccgctg ctctcgaccg actcggcgag cgggccggtc
     5821 tccgcagcgt cggcgatgat gccgaggccg tcacggagcc actggatcgc ggcgccggtc
     5881 gcgaagatcg ccccttcgag cgcgtagtgg gtcacgaggt cggggctctc gccgggggag
     5941 agcatcgagc ccggcaacgt ccacgccacg gtcgtgagta ggccgtcggt cgggacgggc
     6001 agcgtgtcgc cgatgttcat caggacgaac gaaccggtgc cgtacgtgtt cttggtcatg
     6061 ccgacctcga agcacgcctg gccgaacagg gccgactgtt ggtcgccggc gatgcccgac
     6121 accgggatgc cggcgcctcc ggcgaacccg tcggtcgtga ccccgaaccg gcccgcggac
     6181 ggccgaacct cgggcaggac cgaccggggc acaccgaaca gctcgcacaa ctcgtcggac
     6241 cacgttccgg tgccgatgtc gaacaacatg gtgcggctgg cgttcgacgg gtcggtcgca
     6301 tgcactgctc ccgccgctgc cgtgccgccg gtcaggttcc agaggatcca cgagtcgatc
     6361 gtcccgaccg cagtgtccgg cccgaggtcg acgccgccgt gtcgccgcag ccactcgacc
     6421 ttcgagcccg agaagtacgg atcgagcacg agccccgtcg tcgaccggac cagcgggagg
     6481 tggccggcct cgaccagctc gtcgcagcgg gcggcggtgc gccggtcctg ccacacgatg
     6541 gcgcggtgca gcggcatccc ggtggagcg
//
LOCUS       DAOSOA010000178         6510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11934_length_6510_cov_5.578156, whole genome shotgun sequence.
ACCESSION   DAOSOA010000178 DAOSOA010000000
VERSION     DAOSOA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11934_length_6510_cov_5.578156"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKY13_11110"
     CDS             <1..730
                     /locus_tag="PKY13_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80529.1"
                     /translation="IPPDELRYQWTIGAFFVFTFVLLLAATADAPAAWRVSAVRPVIA
                     LTAIAVVANAVIVVGDVGDWNDGVETAVPGVRANLWVAEVAERSGTLERDRALPVSYV
                     RVTAGEYVDAVMALGSPLAGFGADEFGGSADSRRAADEAFVADFDVAMTNGSNGPDDC
                     ERTFTGPGEVSVAAGTIAHVAARSASVDVGFAVFGSGVPLGTVYPDDVSAGVVATPDL
                     PSGSPVSSYRITLSAAAIVAVCDT"
     gene            complement(679..3099)
                     /locus_tag="PKY13_11115"
     CDS             complement(679..3099)
                     /locus_tag="PKY13_11115"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT80530.1"
                     /translation="MTSTATRPSSAATSVARRPGLDVLRGVAATAVVVSHVAFVSGVV
                     NPERWSSQLRLILPRLDVGVPIFFVLSGLLIARPFLRAVADDRPAPAPVRYLLRRAAR
                     IYPLYWVVLAASLAAASAELPSIAKLLSYVGLVHIYRPATAIGPITQSWSLATELSFY
                     LLVPVWFWVCRRVLDRFGVTERERRLRWMGLALVGWVAVALAWRAGVVAATDTFDYTK
                     AGAVDVRGALLTWLPNHLDEFAVGIGLAVLLELRRDRERFGAIGPVGRITCYVIAAAA
                     LWIASAHLDLPPLHTGFDGFQTLARHVLFLVTAALVVAPSALAVPSATGSVLQVRPSG
                     GENAGQNRRGPGENAGQRWGARVAAAVGLGSYGVYLWHQFVADRWMARPGHREFQVAF
                     PTMLVVVLAGSAALAFVGYWLVEKWPQPMVEAVLDAPAAADSGPPRQLGTSPTLDGLR
                     GLSILAVLGTHVIFVDPPNQHGFFRGGFLGVDVFLVLSGFLISATLLRERDRTSTVKL
                     GGFLRRRAERLVPPMVVFLAIHAVVVLILGDSFTEEIRQAVLALTFLSNWQLSFGHQP
                     PYDLVHLWSLAVEGQLYVLCGLAVYWARKRLDRTSLVVGSLVAAAFAVAIWRLVEFRH
                     GVDLEALYQRTDARADSMIIGGLGAVLWRARLVSDDVARRVGVVAGVAIFVSWWWFEG
                     GDGSLFAGGFSLIAVAVVAVILAALVPDRSPVAAVAGWAPLRAIGRMSYSLYLWHLPV
                     YRWVSRGLPDVPWIPRYAIAVVLSFVVAALGYRFVEQPFLRRRRAVAPADQVSHTATM
                     AAADNVIR"
     gene            3130..5037
                     /locus_tag="PKY13_11120"
     CDS             3130..5037
                     /locus_tag="PKY13_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80531.1"
                     /translation="MDEPPSTGAAGRDIPTLVTGLLAGAVVLMGAVASAKGWIPTGDD
                     AFLSLRTRHLFSTDPILLNNASSAGPSAGSQYNHPGAFPLTLLAPVTLLGGPGSLALA
                     TAIVNAAWLSLIAFMVRRIAGPTAQLAALFGLAVFVWSLGGTFLVDPWNPTYGILPVA
                     AMVVAAWGTRLGHTRMLAVTVFAASVAFQTHLSLTVLSAAVAAWALLGAVAAGYRARR
                     DERADDVRGIARALVLAVAVGLVANAQLLVDQFFGTGNLGKIIAGTGFKEATVSVRQI
                     AGVLASKMILPPMWLRGSWDGPVLPGDEPSTGATVLGLIVVAVVTVLAIRSARRRSPE
                     VVTLFGVLAVAFVSASLVATRFPLRIGIPLPYFRWVWPLAAIWAAAVIGSLFSPPVLW
                     RAFAAAGAEARHRTGAIAVAAVTIVVLALAFVPTNVDISPNPAWAQDISRDFSAAAVR
                     ETDKRQPVVIDQSIQEAALWVIPALMDQLDAAGIDVRADDAVLIQQTDDAYRATGDEQ
                     WHLAVRGGLQIDDAPPGGREIARHDALSASQRAELDRLIEDLSPALAEPGAIRLTTTG
                     RAETDAADLGHLTTGELPVEELLRSVPFRLAFDRGAIEAPGVDQDDLRRALDLGALDG
                     GRSIALYLYQP"
     gene            complement(5121..6332)
                     /locus_tag="PKY13_11125"
     CDS             complement(5121..6332)
                     /locus_tag="PKY13_11125"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT80532.1"
                     /translation="MPALFVATPPRPGDIRTSFPYVPALDGIRGLLVFPVLLFHFSVT
                     ASVDGVPAAHLAPGSYFAPSMFFTLSGFLITSLLLIEKQRTAVDGGAGSVDWRGFWRR
                     RFRRLVPASLFVILAAAAIPLVWTNAWGKLPTSNALAAIFSVKNWQDIHYAASADQGL
                     RTLGPLSPYWSLSIEEQFYLGMSIVIGLAMLRVDWRKWLTATFTAIGIASIVAMVVVQ
                     STPNRELFGTDIRASELVAGCLLAMAVQRFGWPTKWWWGVVGFASLAVTFVLWGWVTE
                     QDSWVLNGGLAAVSLLNVGMILGASSWGSSFARAMSFRPLVEIGKLSYPLYLVHWPIA
                     LATQPVHTGLSGWTLMVVRFLLSMVVAWPLAHFVEAPIRKQKVLKGRQFAIAWASVAA
                     LIVVICTIAAD"
BASE COUNT         1084 a         2302 c         2268 g          856 t
ORIGIN      
        1 catcccgccc gacgagttgc gctaccagtg gacgatcggt gcgttcttcg tcttcacgtt
       61 cgtcctcctg ctcgcagcga ccgccgatgc gccggcggcg tggcgcgtca gcgccgtccg
      121 gccggtcatc gcactcacgg cgatcgccgt ggtggccaac gccgtcatcg tggtcggtga
      181 cgtcggcgac tggaacgacg gcgtcgagac ggccgtgccc ggcgtgcggg cgaacctgtg
      241 ggtggcggaa gtcgccgagc gttccggcac gttggaacga gatcgggcgc tgccggtgtc
      301 ctacgtgcgg gtgacggccg gcgagtacgt cgacgcggtg atggcgctgg gcagcccgct
      361 cgccgggttc ggagcggacg agttcggcgg atcggccgac tcgcgacgcg ccgcggacga
      421 ggcgttcgtc gccgacttcg acgtggcgat gaccaacgga tcgaatgggc ccgacgactg
      481 cgagcgcacg ttcaccggcc ccggcgaagt atccgtcgcg gccggaacga tcgcccacgt
      541 ggcagcgcgg tccgcatccg tcgacgtcgg gttcgcagtg ttcgggagcg gggtcccgct
      601 cggcaccgtg taccccgacg acgtgtccgc cggcgtcgtc gccaccccgg acctgcccag
      661 cggctccccg gtgtccagct atcggatcac gttgtcggca gccgccatcg tcgccgtgtg
      721 cgacacctga tcggccggcg ccaccgcccg ccgacgccgc aggaacggct gctcgacgaa
      781 ccggtagccg agggcagcga cgacgaagct gagcaccacc gcgatggcgt agcgcgggat
      841 ccacggcacg tcgggcagac cgcgtgacac ccagcggtac acgggcaggt gccacaggta
      901 cagggagtac gacatgcggc cgatcgcccg cagcggcgcc cacccggcga ccgcagcgac
      961 gggcgaacga tccggtacca gcgccgccag gatcacggcg acgacagcga cagcgatcag
     1021 gctgaaccca ccggcgaaca gggagccgtc gccgccctcg aaccaccacc acgacacgaa
     1081 gatggcgacg cccgccacca caccgacccg gcgggcgacg tcgtcggaaa cgagtcgggc
     1141 acgccacagg acggccccga gcccgccgat gatcatcgag tcggcgcggg cgtcggtgcg
     1201 ttggtagagc gcctcgaggt cgactccatg gcgaaactcg accagccgcc agatcgccac
     1261 cgcgaacgcc gcggcaacga gcgaaccgac gacgagcgac gtgcggtcga gccgcttgcg
     1321 agcccagtag accgcaaggc cgcacaggac gtagagctgc ccctcgacgg cgagcgacca
     1381 caggtggacg aggtcgtagg gcggctggtg gccgaagctc agctgccagt tgctgaggaa
     1441 cgtcaacgcc aggacggcct ggcggatctc ctcggtgaac gagtcgccga gaatcaggac
     1501 gacaacggcg tggatggcga ggaacacgac catcgggggc accagtcgct cggcgcgccg
     1561 acgaaggaac ccgcccaact tcacggtcga cgtgcggtca cgttcgcgca gcagggtcgc
     1621 gctgatgagg aagcccgaca ggaccaggaa cacgtcgacg ccgaggaacc cgccacggaa
     1681 gaacccgtgc tggttcggag ggtcgacgaa gatcacgtgg gtgccgagga ccgcaaggat
     1741 cgacaggcca cggagcccgt cgagggtcgg ggacgtgccg agctgacggg gcgggcccga
     1801 gtcggcggct gccggggcgt cgagcaccgc ctcgaccatc ggttgcggcc acttctcgac
     1861 gagccagtag ccgacgaacg ccagggcggc gctgccggcg agcacgacga cgagcatcgt
     1921 cgggaacgcg acctggaact cgcgatggcc gggacgcgcc atccatcgat cggccacgaa
     1981 ctggtgccac aggtacaccc cgtaggaccc gagcccgacc gccgcggcga ccctcgcccc
     2041 ccacctctgc cctgcgtttt cgcccgggcc tcgccggttc tgtcctgcgt tttcgccccc
     2101 agaggggcga acctgcagga cagaaccggt ggcgctggga acggccaggg ccgagggggc
     2161 gacgacgagc gcagccgtca cgaggaagag gacgtggcgg gcgagggtct ggaacccgtc
     2221 gaacccggtg tggaggggcg ggaggtcgag gtgggccgag gcgatccaga gcgccgctgc
     2281 cgcgatcacg tagcaggtga tccgaccgac cgggccgatc gcgccgaacc gctcgcgatc
     2341 gcgtcgcagt tcgagcagca cggccaggcc gatgccgacg gcgaactcat cgaggtggtt
     2401 cggaagccag gtgagcagcg cgccccgcac gtcgacggca ccggccttcg tgtagtcgaa
     2461 cgtgtcggtc gcagcgacca caccggcacg ccacgccaac gcgacggcga cccaaccgac
     2521 gagcgcgagc cccatccagc gcaggcggcg ctcccgctcg gtcacgccga agcggtcgag
     2581 caccctgcgg cacacccaga accacaccgg caccagcagg tagaacgaca actcggtcgc
     2641 caggctccac gactgggtga tcggcccgat ggcggtggcc ggccgataga tgtggacgag
     2701 gccgacgtag ctcagcagct tggcgatcga gggcagctcg gccgacgctg ccgccagcga
     2761 agcggccagc acgacccagt acagggggta gatccgggcg gctcggcgca ggagatagcg
     2821 aaccggcgcc ggagcgggac gatcgtcggc caccgcccgg aggaacggcc gagcgatcag
     2881 gagccccgac agcacgaaga agatcggcac gccgacgtcg aggcggggca ggatcaaccg
     2941 gagctgcgat gaccaacgct ccgggttcac gacgccgctc acgaacgcca cgtgcgacac
     3001 gacgaccgcc gtcgctgcga caccacgcaa cacgtcgagc ccgggccgac gcgcgaccga
     3061 ggtcgcggcg ctcgagggac gcgtggcggt cgaagtcact ccgttagcat gctccacgcc
     3121 ggagcaccca tggacgaacc cccttccacc ggcgcggccg gacgcgacat cccgacgctg
     3181 gtcacgggcc tcctggccgg cgcggtggtg ctgatgggtg ccgtggcgtc ggcgaagggg
     3241 tggatcccga cgggcgacga cgcgttcttg tcgttgcgca cccgccacct gttctcgacc
     3301 gacccgatcc tgctcaacaa cgcgtcgtcg gccggtccgt cggcggggtc gcagtacaac
     3361 caccctggag cgttcccgct cacactgctc gcgccggtca cgctgctggg cggccctggc
     3421 tcactcgcgt tggcgacggc gatcgtcaac gcggcgtggc tctcgctcat cgcgttcatg
     3481 gtccggcgca tcgccgggcc gaccgcccaa ctcgcagcgc tgttcggcct cgccgtgttc
     3541 gtgtggagcc tgggcgggac gttcctcgtc gatccgtgga accccaccta cggcatcctg
     3601 ccggtcgcgg cgatggtggt cgccgcctgg ggcacgaggc tgggccacac ccggatgctc
     3661 gcggtgacgg tgttcgccgc gtcggtcgcg tttcagacgc acctgagcct gaccgtcctc
     3721 tcggcggccg tcgccgcgtg ggcgctgctc ggcgccgtcg ccgcggggta tcgagcccgt
     3781 cgtgacgagc gtgccgatga cgtccgcggc atcgcccggg cgctcgtgct cgcggtcgcg
     3841 gtcgggctcg tcgccaacgc ccagttgctc gtcgaccagt tcttcggcac cggcaacctc
     3901 ggcaagatca tcgccggcac cgggttcaag gaggcgacgg tctcggtgcg ccagatcgcc
     3961 ggggtgctgg catccaagat gatcctcccg ccgatgtggc tgcggggcag ctgggacggg
     4021 ccggtgctcc cgggagacga accatcgacc ggggccaccg tgttgggcct gatcgtggtc
     4081 gcggtcgtga cggtcctggc gatccgatcg gcccggcggc gctcccccga ggtcgtcacc
     4141 ctgttcggtg tacttgcggt cgcgttcgta tcggcatcac tcgtcgcgac ccggttcccg
     4201 ctccgcatcg gcatccccct tccgtacttc cgctgggtct ggcccctcgc cgccatctgg
     4261 gcggcagccg tcatcggctc cctcttcagc ccaccggttc tgtggcgtgc gttcgctgct
     4321 gcaggtgccg aagcacgcca cagaaccggc gcgatcgcgg tggccgcagt gacgatcgtg
     4381 gtcctggcgc tggcattcgt gcccacgaac gtcgacatct cccccaatcc ggcgtgggcg
     4441 caggacatct cccgcgactt cagcgccgct gccgttcgcg agaccgacaa acgccaaccc
     4501 gtcgtgatcg accagtcgat ccaggaggcc gcgctgtggg tgatcccggc gctgatggac
     4561 caactcgacg ccgccggcat cgacgtgcgc gccgacgacg cggtgctcat ccaacagacc
     4621 gatgacgcct accgagcgac tggtgacgag caatggcacc tcgccgtgcg cggggggctg
     4681 cagatcgacg atgcgccgcc cggcggccgg gagatcgccc gtcacgacgc gttgtcggcg
     4741 tcgcaacggg ccgagctcga tcgcctgatc gaggacctga gcccggcgct ggcggagccc
     4801 ggcgctatcc ggctcacgac cacgggtcgc gccgagaccg acgccgccga cctcgggcac
     4861 ctcaccaccg gcgagctgcc cgtcgaggag ctgttgcgct ccgtgccgtt ccgcctcgcg
     4921 ttcgatcgtg gggcgatcga ggcgcccggt gtcgatcagg acgatctccg gcgggcgctc
     4981 gacctcggcg ccctcgacgg cggccgctcg atcgccctct acctgtacca accctgaccc
     5041 gatctcccac gccgaccggt tctgtcccgc gaacgcgtgc agaccgaatc gcttcgcggg
     5101 acaaaaacag cggggccggg tcagtcggcg gcgatcgtgc agatgaccac gatcaacgcg
     5161 gcgaccgacg cccaggcgat cgcgaactgc cgccccttca gcaccttctg cttgcggatc
     5221 ggcgcttcga cgaagtgggc cagcggccag gccaccacca tcgacagcag gaaccggacg
     5281 accatgagcg tccagcccga caggccggtg tgcacgggct gggtcgccag cgcgatgggc
     5341 cagtgcacca ggtacaaggg gtagctgagc ttgccgatct cgacgagcgg ccggaacgac
     5401 atcgcccggg cgaacgacga cccccacgac gaggccccga ggatcatgcc gacgttgagc
     5461 agcgacacgg cggccaaccc gccgttcagc acccacgaat cctgctcggt cacccatccc
     5521 cagagcacga acgtgaccgc caggctcgcg aacccgacca ccccccacca ccacttcgtc
     5581 ggccagccga accgctgcac cgccatggcc aacagacagc cggcgacgag ctccgacgcc
     5641 cggatgtcgg tgccgaacag ctcccggttc ggtgtcgact gcaccaccac catcgccacg
     5701 atcgaggcga tgccgatggc tgtgaacgtt gcggtcagcc actttcgcca gtcgacgcgc
     5761 agcatggcga ggccgatgac gatcgacatg ccgaggtaga actgctcttc gatcgacagg
     5821 gaccagtatg gcgacagcgg gcccagcgtc cgcagtccct ggtccgcgga tgcggcgtag
     5881 tggatgtcct gccagttctt gaccgagaag atggcggcaa gcgcattcga ggtggggagc
     5941 ttgccccacg cgttcgtcca gaccagcggg atggccgccg cggccaggat cacgaacagc
     6001 gacgccggga cgagtcgacg gaagcggcgg cgccagaacc cccgccagtc gaccgatccg
     6061 gccccaccgt cgacggctgt gcgctgcttc tcgatcagga gcagcgacgt gatgaggaac
     6121 cccgacaacg tgaagaacat cgacggggcg aaatacgaac cgggcgcgag gtgtgccgcc
     6181 gggaccccgt cgaccgatgc ggtgaccgag aagtggaaca acaacaccgg gaacaccagg
     6241 agcccgcgga tcccgtcgag cgccggaacg taggggaacg acgtccggat atcgcccggt
     6301 ctggggggtg tggcgacgaa cagggcgggc acgaaggggg aacctagtgg ggccgggtgg
     6361 cagcccttga ggatggcccg aacccggggc gcgagtaact tcgtccaccg tgccgatccg
     6421 tcgtttccag cgtccgcagt tcgggcgccc ccgcgtcggt gacgtgatgt tggcggtcgt
     6481 ctcgtacgtc ccgctgctcc tgacgcaccg
//
LOCUS       DAOSOA010000179         6503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_11951_length_6503_cov_5.905707, whole genome shotgun sequence.
ACCESSION   DAOSOA010000179 DAOSOA010000000
VERSION     DAOSOA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11951_length_6503_cov_5.905707"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /gene="glnS"
                     /locus_tag="PKY13_11130"
     CDS             <1..1530
                     /gene="glnS"
                     /locus_tag="PKY13_11130"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOT80533.1"
                     /translation="IAEEFDGTCFLRFDDTNPATEDVSYVDAIQEDLAWLGVTPSGPP
                     RYASDYFEQIYEWAEELVRKGLAYVDDQDKETISEQRGGFGQPGVESPHRDRSVEENL
                     GLFRQMRDGVFADSEKVLRAKIDMQADNMWLRDPVLYRIRRVPHHRTDDRWVIYPTYD
                     WAHGQSDAIEGTTHSICTLEFTDHRALYDWCLDQLELPFDRPEQTEFARLNLTHTVLS
                     KRVLRTLVEQGHVDGWDDARMPTVRGLRRRGYPAPALRAFTDHISVAKTNSVVDIELL
                     EYFVRTHHNLHALRRMVVLEPLKLTITNWPTDVDGTAHVEWMDVVNNPEDESAGTRSV
                     PFTGELWIEQDDFRLEPPPKYFRLTPGREVRLRNAFLVTATEAVLDDEGNVVEVLATY
                     DPETLGGTAPDGRKVKSTMHWVSRPHAIDATVHRYERLFRSPHPAAEGRDPLDDLDPD
                     SRQTTRAKAEPALAEVETGQVVQFERLGYFAADLDDTTVFHRTVGLKDEWANLQKART
                     T"
     gene            complement(1562..3781)
                     /locus_tag="PKY13_11135"
     CDS             complement(1562..3781)
                     /locus_tag="PKY13_11135"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOT80534.1"
                     /translation="MASSKTTDAGTTTTGTTPPPRAKAPSRRRPSRGATTSTEPQPSG
                     TPVGGTVEGDPTAPATAPAKVSAKTGSAPRNTAVDPAWAKQGDALTTSQGQRVEDTDN
                     SLRAGRRGPSLLEDHHLREKITHFDHERIPERVVHARGAGAHGHFELFESLESVTSAR
                     VLTEVGVSTPVFTRFSTVAGSRGSADTARDVRGFAVKMYTSEGNWDLVGNNIPVFFIQ
                     DGIKFPDLIHAAKPEPDREIPQAQTAHDTFWDFVSLQPESTHMLMWAMSDRGIPRSYR
                     TMEGFGVHTFRLINAAGESSLVKFHWKPTVGIHSLVWEESQKLGGIDPDFHRRDLWNA
                     IEAGAFPSWDLGVQIMPDTDDQTFEGIDLLDATKIVPEELCPVRLVGRMTLDANPTNY
                     FAETEQVAFHPGHVPPGIDVTDDPLLHARLFSYIDTQITRLGGPNFAQLPINRAAAPV
                     NDNLRDGFGQQAIHSGRTAYSPNTIGGGCPFATGDAPFVHFPQPVDGVKARKRPQSFD
                     DHFSQATLFWNSLTEVERDHEVDAFSFELSKVADPGVVARMVGNLANVDPDLASSVAA
                     NLGLVAPSGSPEGDAGASPALSLVPSASGPIDGRVVGVLVTPGADIGGVAALRKVLDA
                     AGAAMHVIAPTAGSVKGSGRSTLAADATVFNSDSVVYDALVVAGGVAELDPKSAMMLQ
                     EAYRHHKTVAAWGTGTDALAAASIDQTANGVVTGDKVAKGFTTALVDAIGWHRHWAR"
     gene            3905..4468
                     /locus_tag="PKY13_11140"
     CDS             3905..4468
                     /locus_tag="PKY13_11140"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HOT80535.1"
                     /translation="MTTRVAFLRAVNVGKRTVAMARVVEIFEELGYEQVWTYINSGNV
                     VFDATGSRAEHERRIGDALAAEYGFEVTTFVRTAAQIRAVLAAQPFEVVDGDTYFVTF
                     LQATPSVAVATEFEACSNDVDSVVVDGAEVHWRMHGSSMDTTLGKKHWKLLGEFSTSR
                     NTTMLHKLSDRIVARSGAAQPGSSSRK"
     gene            complement(4441..4914)
                     /locus_tag="PKY13_11145"
     CDS             complement(4441..4914)
                     /locus_tag="PKY13_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT80536.1"
                     /translation="MDPAPIVRLADPSDAVELAGLLHDFNTEFDTPSPGPAVLADRLA
                     TLLGGDRTLALLAGAPAVGVALVTLRPNVWYEGPVALLDELYVRPALRSRGIGTAIVD
                     RLGVECARRGVDAIEINVDEGDVDAQRFYERQGFSGVEPTTGERAFYYFRELDPG"
     gene            complement(4961..5929)
                     /locus_tag="PKY13_11150"
     CDS             complement(4961..5929)
                     /locus_tag="PKY13_11150"
                     /inference="COORDINATES: protein motif:HMM:NF025847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4436 family protein"
                     /protein_id="HOT80537.1"
                     /translation="MPRLRPRRPRSMSLRRRRPTKTQLAALALVTILSFGPIVALLAV
                     IGRDSGPVASLSTGRTPPDDGAKVDVSFTAVSPSTGTMTALVQVEPFPGLLDEQGLTD
                     ELVMRVNDARGDTSIVFPAGEPLRAVTVTLPLSGRSISRYPFDRYRSAVLMRLEHPTG
                     AETEPEPVRFTVDVSSQLADFDVGLDDFTDGDTRAAPAVGVGFELSRATTTTVYAVGI
                     MILMWGLALAGVAILWAVTIWGIEVPFWSVGYLVGVLFALAPLRASLPGSPPPGTLLD
                     FVAFYWSIGLVGLTLIMVMAVWLQRARPAKPAPAPPDDEETATNPS"
     gene            complement(5970..>6503)
                     /locus_tag="PKY13_11155"
     CDS             complement(5970..>6503)
                     /locus_tag="PKY13_11155"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HOT80538.1"
                     /translation="GVPGEMVDAEPSRFFHPPTTGASSAWLGVYLDGTGEDAVDWDEI
                     AAMIADAHMTVTGSTTASGHAAAGHEVADSPGTFRFTARLWIHDGGSWYFATVPPVIA
                     TAIDLLQAEERRGFGSRRVHVTIGATSWDTSIFPDAATKTYVLPVKKAVRTAERLDEG
                     DDAHVTIELVDVTDARG"
BASE COUNT         1001 a         2191 c         2325 g          986 t
ORIGIN      
        1 atcgccgagg agttcgacgg cacctgcttc ctccggttcg acgacaccaa ccccgcgacc
       61 gaggacgtgt cctacgtcga cgcgatccag gaggatctgg cgtggctcgg tgtcacgccg
      121 tcgggtccgc cccgctacgc gtcggactac ttcgagcaga tctacgagtg ggccgaggag
      181 ctcgtgcgga aggggcttgc ctacgtcgac gaccaggaca aggagacgat ctccgagcag
      241 cggggtgggt tcggccagcc cggcgtcgag agcccccatc gcgaccgctc cgtcgaggag
      301 aacctcggcc tgttccggca gatgcgcgac ggcgtcttcg ccgacagcga gaaggtgctc
      361 cgcgccaaga tcgacatgca ggccgacaac atgtggctgc gtgacccggt gctgtaccgc
      421 atccgtcgtg tgccccacca ccgcaccgac gaccggtggg tcatctaccc gacctacgac
      481 tgggctcacg gccagtccga cgccatcgag ggcaccacgc actcgatctg caccctcgag
      541 ttcaccgacc accgggcgct ctacgactgg tgcctcgacc agctcgagct gcccttcgac
      601 cggcccgagc agaccgagtt cgcccggctc aacctgaccc acaccgtgct gtcgaagcgg
      661 gtgttgcgca cgctggtcga acagggtcac gtggacggct gggacgacgc ccggatgccg
      721 acggtgcgtg gcctgcgtcg ccgtggctac ccggcgcccg cgttgcgggc gttcaccgac
      781 cacatctcgg tggccaagac caactccgtg gtggacatcg agctgctcga gtacttcgtg
      841 cgaacgcacc acaacctgca tgcgctccgg cggatggtgg tcctcgaacc gctgaaactc
      901 acgatcacga actggcccac cgacgtggac ggcacggccc acgtcgagtg gatggacgtg
      961 gtcaacaacc ccgaggacga gtcggccggc acccgttcgg tgccgttcac cggcgagctc
     1021 tggatcgagc aggacgactt ccggctcgag cccccaccca agtacttccg gctcacaccc
     1081 ggccgggagg tccggctgcg gaacgcgttc ctcgtgaccg ccaccgaggc cgtgctcgac
     1141 gacgagggca acgtcgtcga ggtgctcgcc acctacgacc ccgaaacgct cggcggcaca
     1201 gcacccgacg gtcgcaaggt gaagtcgacg atgcactggg tgtcccggcc gcacgcgatc
     1261 gacgcgacgg tgcaccgtta cgagcgcctc ttccgatcgc cgcaccccgc tgcggagggc
     1321 cgcgacccgt tggacgacct cgatcccgac tcccgccaga cgacccgggc gaaggccgaa
     1381 ccggcgctcg ccgaggtcga gaccgggcag gtcgtgcagt tcgaacggct ggggtacttc
     1441 gccgcggacc tcgacgacac gaccgtgttc caccgcaccg tcggcctgaa ggacgagtgg
     1501 gccaacctgc agaaggctcg cacgacctga ccgccgtgcc caggctgccc tggtcgaggg
     1561 gtcagcgcgc ccaatgccgg tgccagccga tcgcgtcgac gagcgccgtg gtgaagcctt
     1621 tcgccacctt gtcacccgtc accacgccgt tcgccgtctg atcgatgctc gctgcggcga
     1681 gcgcgtcggt gccggtaccc caggcggcca cggtcttgtg gtggcggtag gcctcctgca
     1741 gcatcatcgc cgacttcggg tcgagctcgg cgaccccgcc tgcgacgacc agggcgtcgt
     1801 agaccacgga gtcggagttg aagacggtgg cgtcggcggc gagggtcgaa cggccggagc
     1861 ccttgaccga accggcggtc ggtgcgatga cgtgcatcgc cgcaccggcg gcgtcgagca
     1921 ctttgcgcaa cgctgcgacg ccgccgatgt cggcgccggg cgtgaccagc acgccgacga
     1981 cccgaccgtc gatcgggcca ctcgccgacg ggacgagcga cagggcgggc gatgccccgg
     2041 cgtcaccctc gggtgaaccc gacggggcga cgagaccgag gttggcggcg accgacgacg
     2101 cgaggtcagg gtcgacgttg gcgaggttcc ccaccattcg cgccacgacc ccggggtcgg
     2161 cgaccttcga cagctcgaag ctgaacgcgt ccacttcgtg gtcacgttcc acctcggtga
     2221 ggctgttcca gaacagggtc gcctggctga aatggtcgtc gaaggactgc ggccgcttgc
     2281 gggccttgac gccgtcgacg ggctggggaa agtgcacgaa cggcgcgtcg ccggtggcga
     2341 aggggcagcc gcccccgatc gtgttcggcg agtaggcggt tcgaccgctg tggatcgcct
     2401 gctggccgaa tccgtcgcgc aggttgtcgt tcaccggcgc cgctgcccgg ttgatgggga
     2461 gctgggcgaa gttcggcccg ccgagacggg tgatctgggt gtcgatgtag gagaagagac
     2521 gggcatgcag cagcgggtcg tcggtgacgt cgatgcccgg cggcacgtgg ccgggatgga
     2581 acgccacctg ttcggtctct gcgaagtagt tggtcgggtt ggcgtcgagc gtcatccgtc
     2641 cgacgagccg gaccgggcac agctcctcgg ggacgatctt cgtggcgtcg aggagatcga
     2701 tgccctcgaa cgtctggtcg tcggtgtcgg gcatgatctg cactcccagg tcccacgaag
     2761 ggaacgcgcc ggcctcgatc gcgttccaca ggtcacgacg gtggaagtcg gggtcgatgc
     2821 cgcccagctt ctgggactcc tcccagacga ggctgtggat gccgacggtc ggcttccagt
     2881 ggaacttcac gagcgacgac tcgccggctg cgttgatcaa ccggaacgtg tggacgccga
     2941 agccttccat cgtgcggtac gagcggggga ttccgcggtc cgacatggcc cacatcaaca
     3001 tgtgggtgct ctcgggctgg agcgacacga agtcccagaa cgtgtcgtga gcggtctgtg
     3061 cctgcgggat ctcgcgatcg ggctccggct tggcggcgtg gatcaggtcg gggaacttga
     3121 tgccgtcctg gatgaagaac accgggatgt tgttgccgac gagatcccag ttgccctccg
     3181 acgtgtacat cttcacggcg aagccccgca cgtcgcgggc cgtgtcggcc gatccgcggg
     3241 aaccggcgac ggtcgagaag cgggtgaaca ccggtgtgct gacccctacc tcggtcagga
     3301 cacgcgccga cgtcacggac tcgagcgact cgaacagttc gaagtggccg tgggcgccgg
     3361 cgccgcgggc gtgtaccacg cgctcgggga tgcgttcgtg gtcgaaatgg gtgatcttct
     3421 cgcggaggtg gtggtcttcc aacagcgacg gaccccgacg gccggcccgg aggctgttgt
     3481 cggtgtcctc cacgcgctgg ccctggctcg tggtgagcgc atcaccctgc ttcgcccacg
     3541 ccgggtcgac ggcggtgttg cgcggtgcgg agcccgtctt ggcgctcacc ttcgccggtg
     3601 cggtggctgg tgccgtcgga tcgccctcga ccgttccgcc gacgggggtg ccgctcggct
     3661 gcggttcggt cgacgtggtc gcaccacgcg agggccgtcg ccgcgagggg gccttcgccc
     3721 ggggcggcgg ggtggtgccc gtcgtggtcg ttccggcgtc ggtcgtcttg gaggaggcca
     3781 tgtgctgtga gtccttcgtg tcggcgtcgt tgccctgctg cccgacggcc ggctacggca
     3841 gaccccccga ccgggtgaac cgacgccatc ggtcgccggg gcggcgcgct gccatgatgt
     3901 cggcatgacc actcgtgtcg cgttcctccg tgccgtcaac gtcggcaagc gcaccgtcgc
     3961 gatggcgcgg gtcgtcgaga tcttcgagga gctgggctac gagcaggtgt ggacctacat
     4021 caacagcggc aacgtcgtgt tcgacgcgac cgggtcgcgt gcggagcacg aacgccgcat
     4081 cggtgacgcg ctcgccgccg agtacgggtt cgaggtcacc acgttcgtcc gcaccgcagc
     4141 gcagatccgc gccgtgttgg cggcgcagcc gttcgaggtc gtcgacggcg acacgtactt
     4201 cgtcacgttc ctccaagcga cgccgagcgt cgccgtggcc accgagttcg aggcgtgctc
     4261 caacgacgtc gactccgtcg tcgtcgacgg tgcggaggtc cactggcgga tgcacggttc
     4321 gtcgatggac accaccctgg gcaagaagca ctggaagttg ctcggcgagt tcagcaccag
     4381 ccgcaacacg acgatgctgc acaagttgtc cgaccgaatc gtcgcccggt ccggcgccgc
     4441 tcagccggga tccagctcgc ggaagtagta gaaggcccgc tccccggtgg tcggttcgac
     4501 gccggagaat ccctgacgct cgtagaagcg ttgagcgtcg acgtcgccct cgtccacgtt
     4561 gatctcgatg gcgtcgacac cccggcgggc acactcgacg ccgagtcgat cgacgatcgc
     4621 cgtaccgatg ccccgactgc ggagcgccgg ccggacgtac agctcgtcga gcagggcgac
     4681 tggcccctcg taccagacgt tggggcggag ggtcacgagt gcgacgccga cggcgggtgc
     4741 cccggcgagg agggcgagcg tgcggtcgcc gccgagcagc gtcgccaggc ggtcggccag
     4801 cacggcgggg cccggcgacg gcgtgtcgaa ctcggtgttg aagtcgtgca ggagtccggc
     4861 cagctcgacc gcgtcgctcg ggtcggcgag tcgcacgatg ggtgctgggt ccatgccccg
     4921 atgctacggg ctggtcccgc cgcatccgcg agctgctggc tcacgacgga ttggtcgccg
     4981 tctcctcgtc gtcgggcggt gccggggccg gcttggccgg gcgcgctcgt tgcagccaca
     5041 ccgccatcac catgatcaat gtgagcccga cgaggccgat cgaccagtag aaggccacga
     5101 agtcgagcaa cgtgccgggc gggggactgc cggggagcga cgctcgcagc ggggcgagag
     5161 cgaacagcac accgacgagg tagcccaccg accagaacgg cacctcgatg ccccagatcg
     5221 tcactgccca caggatggcg accccggcga gggcgaggcc ccacatcagg atcatgatcc
     5281 ccaccgcgta cacggtggtg gtcgtggccc gtgacagctc gaacccgacg ccgacggcgg
     5341 gcgccgcccg ggtgtcgccg tcggtgaagt cgtcgaggcc gacgtcgaag tcggccagct
     5401 gcgacgagac gtcgacggtg aagcggacgg gttcgggctc ggtctcggca ccggtcgggt
     5461 gctccagccg catcagcacg gcggaccggt agcggtcgaa cgggtacctc gagatggaac
     5521 gaccgctgag cggcagcgtg acggtgacgg cccgcagcgg ttcgccggcc gggaacacga
     5581 tcgacgtgtc accgcgggcg tcgttgacgc gcatcacgag ctcgtcggtc aggccctgct
     5641 cgtcgagcag cccggggaac ggctcgacct ggaccaacgc ggtcatggtg cccgttgacg
     5701 gggacacggc cgtgaacgac acgtcgacct tggccccgtc gtcgggtggg gtgcggcccg
     5761 tcgacaggga ggcgaccggg cccgagtccc tcccgatcac ggccaggagg gccacgatcg
     5821 ggccgaagga caggatcgtg acgagcgcga gcgctgccag ctgcgtcttg gtcggccgac
     5881 ggcgccgcag cgacatcgac cgcggtcggc gtggtcgcag tcggggcacc catgcgacca
     5941 taccggggtc ggcgtccgac gtccggccgt cagcctcggg cgtcggtcac gtcgaccagc
     6001 tcgatcgtga cgtgggcgtc gtcgccctcg tcgagccgct ccgcggtccg aacggccttc
     6061 ttgaccggga gcacgtacgt cttggtggcg gcgtcgggga agatcgacgt gtcccacgac
     6121 gtggccccga tcgtgacgtg gacacgccgg gaaccgaagc cgcgtcgttc ctcggcctga
     6181 agcagatcga tcgctgtggc gatcaccggt gggacggtgg cgaagtacca ggagccgccg
     6241 tcgtggatcc acaggcgggc ggtgaaccgg aacgtgccgg gggagtcggc cacctcgtga
     6301 ccggccgcag cgtgaccgga cgcagtggtc gaaccggtga cggtcatgtg ggcgtcggcg
     6361 atcatggcgg cgatctcgtc ccagtcgacc gcgtcctcgc ccgtgccgtc gaggtacacc
     6421 ccgagccacg ccgatgacgc tcccgtcgtg ggcgggtgga agaaccgcga cggttcggcg
     6481 tcgaccattt cgccgggcac ccc
//
LOCUS       DAOSOA010000180         6477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12018_length_6477_cov_5.498131, whole genome shotgun sequence.
ACCESSION   DAOSOA010000180 DAOSOA010000000
VERSION     DAOSOA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12018_length_6477_cov_5.498131"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKY13_11160"
     CDS             <1..807
                     /locus_tag="PKY13_11160"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HOT80539.1"
                     /translation="VERVDGGDQRIVAGAPLSGSSLAAFLDLHVEPRAELVADRSGAL
                     TARTTRNVGSIVIGVDAGHAPDRLQVAAAAAAVLRERGAALLGWSDTARDLQRRAVFA
                     RLADDRVPDVSDEALVASAPSWIGSFLPDGPRVDLGAISAGDVLAAAIGASRSRIDEL
                     TPTHFVVPSGRRHRIEYGPDGPVVRVKLQEMFGSPVSPTVGAGTVPVTLHLLSPAGRP
                     LQVTQDLASFWANAYAQVRSEMRGRYPRHPWPEDPTAAVPTASVKHPRKR"
     gene            complement(857..1804)
                     /locus_tag="PKY13_11165"
     CDS             complement(857..1804)
                     /locus_tag="PKY13_11165"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT80540.1"
                     /translation="MTTAAPFSNNRFCALAGVDVPIVQAPMGWIARAQLASAVSNAGA
                     LGIIETSSGELDVVRGEIEMMRELTDRPFGVNIAQAFVRDPSIVEFVVDQGVRLVTTS
                     AGSPMKYTASLKAAGATVFHVVPTLAAALKAIEAGVDGLVVEGGEGGGFKNPRDVATM
                     VLLPLVRSHTDLPIVAAGGITDGPSMAAAFALGADAVQMGTRMVSALESPVHDNWKQA
                     ILAATDTGTVMLNRHTSPALRALRTGRTEALEFSDDNAMAQFGNPTDLYFGGDMESAI
                     PLSGQVAGRIDEILPVQEIVDRTVAGFTATIERLSAGVL"
     gene            complement(1920..3431)
                     /gene="guaB"
                     /locus_tag="PKY13_11170"
     CDS             complement(1920..3431)
                     /gene="guaB"
                     /locus_tag="PKY13_11170"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT80541.1"
                     /translation="MAQLPTDELGGDAAFNARFGRIGLTFDDVLLMPAESNVLPHESD
                     TSTWFTPDIRLAVPIVSAAMDTVTESRMAIALARLGGIGVIHRNLPPEEQAAEIDRVK
                     RSESGMITDPVTLQPHLPVQAALDLMERFHISGIPITDEKNRLVGILTNRDLRFETDF
                     SRRIDEVMTSEGLHTVAVGTTLDQAQEVFRHIKVEKLPVVDADGFLRGLITIKDLQKR
                     TDFPNATKDAEGRLRVAAAVGTGTDVFDRAKQLIDAGVDAIVVDTAHGHATSVAETVR
                     EMRKIWDGPLIAGNVATAAATEALIDAGADAIKVGIGPGSICTTRVVAGIGVPQITAV
                     FDCAQVARRRGRTIIADGGLQFSGDVAKALAAGADCAMFGSLLAGTDESPGDTVLYQG
                     ERYKSYRGMGSLGAMKARSFSKDRYFQEHVVDAEKLVPEGIEGRVAYVGSVGNIVHQL
                     VGGLQQSMGYCGAPTIEALKEAQFIRMTAAGLREAHPHDITITEQAPNYRGGF"
     gene            complement(3490..4032)
                     /locus_tag="PKY13_11175"
     CDS             complement(3490..4032)
                     /locus_tag="PKY13_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80542.1"
                     /translation="MAHHPTLADSSNAPAPPVGPSPRRQKAQRAALVAIAVAFAGVSY
                     WAADGTGGPSIVPARYVVDIVNTCGPGLGAKLSDDPMSTMGDGRPEVDLAVSADVPPI
                     SEGTTTLERPGRGDPQDDQLIVVRFDASASSPSLDGIDGALPTTVASFPMSDQVFDEV
                     DGVMRTTFELGGPRCAQLGE"
     gene            complement(4093..4704)
                     /locus_tag="PKY13_11180"
     CDS             complement(4093..4704)
                     /locus_tag="PKY13_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80543.1"
                     /translation="MIGHRVRSLPRCDLVVVDGIRCTNVARTLVDLGDVVERDRVERA
                     FDHSIRTGANPRWLRQTAERLVRPNVVGPALLLDLLDRHEQRGTVRGSWFERMVELCL
                     VHPELDGLVIQYELRNAEGGHVATFDLAVPDAMLGIEAHSREHHFGAENEASDDHRDN
                     AAGVEAWDVMYLGYESVQSPSAVRDLVVARVRARRRLIGGSAS"
     gene            complement(4701..5042)
                     /locus_tag="PKY13_11185"
     CDS             complement(4701..5042)
                     /locus_tag="PKY13_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOT80544.1"
                     /translation="MRNDAVSVVSKLAASPNNVFTSAQAAAHSLTRRQLRSAIERGLI
                     VEPHRGVFVGHVPDGGPTWRQQIAAATAGRDGAVASHRSAARLARLEGFEHEPSIEAS
                     IERPRLVRLDG"
     gene            5225..>6477
                     /locus_tag="PKY13_11190"
     CDS             5225..>6477
                     /locus_tag="PKY13_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80545.1"
                     /translation="MTMTATTPSPAVPAVGAAGAAGPERRPSLLRTDTPGRMRVWSIA
                     VAVVAVIFGIVGSTAIANRATDLGDARDAAAQLERLESVRTAVVEADSLAASAYLEGG
                     LESPDRRATYEDRLDAAQAGLAAAAARATDAEVEQLGGLASALTTAAGLIEQSRANSR
                     QGFPVGAAYQRNASALIRSDVLPELDSIATATAARTSDEVDRGSRAQLVLWGPALVAL
                     GLIGAASVWLTRRTRRVVNVGLAIAALAVLGFTGFASSVLASSTSNARATLDGSYAGA
                     TAFAQARTAAFDARSNEALTLIARGNGAAFETRWQEQAAQVAAGLTDAAKADSVNGQN
                     AIDGFGSYGSEHVQVRELDDAGDYDTAVERALAVGNGGDGFFQFDEASRTGLESLSAD
                     TDAALGEARDSVSGHRWLVLVAGFVA"
BASE COUNT          933 a         2250 c         2302 g          992 t
ORIGIN      
        1 gtcgagcggg tcgacggcgg cgatcagcgc atcgtggccg gggcacccct gtcgggctcc
       61 tcgctcgccg cgttcctcga ccttcacgtc gagcctcggg ccgagctcgt ggcggaccgg
      121 tccggtgcgc tcacggcgcg caccacccgc aacgtggggt cgatcgtgat cggggtcgac
      181 gccgggcacg ctcccgatcg cctccaggtc gccgccgccg ctgcggccgt gctccgtgag
      241 cggggtgcgg cgctgttggg atggagcgac acagcgcgcg acctgcagcg ccgagccgtg
      301 ttcgcccgcc tcgccgacga tcgggttcct gacgtgtccg acgaggcact cgtggcgtcg
      361 gcgccctcgt ggatcgggtc gttcctgccg gacggtccga gggtggacct cggcgccatc
      421 tcggccgggg acgtgctcgc tgcggcgatc ggtgcgtctc gaagccggat cgatgagctc
      481 acgccgacgc atttcgtcgt cccgtcgggt cggcgtcacc gcatcgagta cggccccgac
      541 gggccggtcg tgcgggtgaa gttgcaggag atgttcggct cgccggtgtc cccgacggtc
      601 ggcgccggga cggttccggt cacgctccac ctgttgtcgc cggccggtcg tccgttgcag
      661 gtcacccagg acctggcgtc gttctgggcg aacgcgtacg cccaggtccg ctccgagatg
      721 cggggtcgct atccccgcca cccctggccc gaggacccga ccgccgccgt gcccaccgcc
      781 tcggtcaagc acccacggaa gcgctgatcc tcggggcttt gtgctgcgag gagcgacgcc
      841 agcgtcgctc ctcgcactac agaaccccag cgctgaggcg ctcgatcgtc gccgtgaacc
      901 cggcgacggt gcggtcgacg atctcctgga cggggaggat ctcatcgatg cggcccgcca
      961 cctggcccga cagcgggatc gccgactcca tatcaccgcc gaagtagaga tcggtcgggt
     1021 tgccgaactg cgccatcgcg ttgtcgtcgg agaactcgag cgcctcggtg cgcccggtgc
     1081 ggagcgcccg cagcgccggg ctggtgtggc ggttgagcat cacggtgccg gtgtcggtcg
     1141 ccgcgaggat cgcctgcttc cagttgtcgt gcaccggcga ctcgagtgcc gacaccatgc
     1201 gtgtgcccat ctgcacggcg tcggcaccga gtgcgaacgc cgcagccatc gacgggccgt
     1261 cggtgatgcc accggccgcc acgatcggca ggtcggtgtg ggaccgcacg agaggcagca
     1321 gcaccatcgt ggcgacgtca cgggggttct tgaagccgcc gccctcgccc ccttcgacga
     1381 cgagaccgtc cacgccggcc tcgatcgcct tcagcgccgc agcgagcgtg ggcaccacat
     1441 ggaagaccgt tgcgccggcg gccttgagcg acgccgtgta cttcatcgga gatccagcgg
     1501 acgtcgtcac cagccgcact ccctggtcga ccacgaactc gacgatcgag ggatcacgca
     1561 cgaacgcctg ggcgatgttg accccgaacg gtcggtcggt gagctcgcgc atcatctcga
     1621 tctcgccgcg gaccacatcg agctcgcccg acgacgtctc gatgatgccg agggccccgg
     1681 cgttcgacac ggccgacgcc agctgggcac gggcgatcca gcccatcggg gcctgcacga
     1741 tcggcacgtc gacgccggca agtgcgcaga accggttgtt cgagaagggc gccgcggtgg
     1801 tcatcctcgt caggtcagtc gacagccgcc ccggatgcaa ggactaccaa gcctcggtac
     1861 cgcagcgtac gaagcgagcg cagcgagcga gcctgagcgg aggtgaccga ggccgtgttt
     1921 cagaacccgc cgcggtagtt gggggcctgc tcggtgatcg tgatgtcgtg gggatgggct
     1981 tcgcgcagcc ctgcggccgt catccggatg aactgggctt ccttgagcgc ctcgatcgtc
     2041 ggggcgccgc agtagcccat cgactgctgc aggccaccga ccagctggtg gacgatgttg
     2101 cccaccgatc cgacgtaggc cacgcgccct tcgatgccct cgggcaccag cttctcggcg
     2161 tcgacgacgt gctcctggaa gtagcggtcc ttcgaaaacg agcgggcctt catggcgccg
     2221 agcgacccca tgccccgata gctcttgtac cgctcgccct ggtacagcac cgtgtcgccc
     2281 gggctctcgt cggtgccggc gagcagcgag ccgaacatgg cgcagtcggc gccggccgcg
     2341 agcgccttgg ccacgtcgcc ggagaactgg agaccaccgt cggcgatgat cgtgcgacca
     2401 cgacggcggg cgacctgggc gcagtcgaac acggccgtga tctgcggcac gccgatgccg
     2461 gcgaccaccc gcgtggtgca gatcgagccc gggccgatgc ccaccttgat ggcgtcggcg
     2521 ccggcgtcga tgagggcctc ggtcgcagcg gccgtggcga cattgccggc gatgagcggg
     2581 ccgtcccaga tcttgcgcat ctcgcgcacc gtctcggcga ccgacgtggc gtggccgtgc
     2641 gccgtgtcga cgacgatcgc gtcgaccccg gcgtcgatga gctgcttggc acggtcgaag
     2701 acgtcggtgc cggtgccgac cgcggcggcg acgcggaggc gaccctcggc atccttggtc
     2761 gcgttcggga agtcggtgcg cttctggagg tccttgatgg tgatgaggcc tcgcaggaac
     2821 ccgtcggcgt cgacgacggg cagcttctcg accttgatgt gccggaacac ctcctgggcc
     2881 tgatcgagcg tggtgccgac ggccacggtg tgcaggccct ccgacgtcat gacctcgtcg
     2941 atgcgacggc tgaagtcggt ctcgaagcgg aggtcacggt tggtgaggat gccgaccaga
     3001 cggttcttct cgtcggtgat cgggataccg gagatgtgga agcgctccat gaggtcgagc
     3061 gctgcctgca ccggcaggtg cggctgcaat gtgacggggt cggtgatcat gcccgactcc
     3121 gaacgcttga cccggtcgat ctcagcggcc tgctcctcgg gaggcaggtt gcggtggatg
     3181 acgccgatgc caccgaggcg cgccagggcg atggccatgc gcgactcggt gacggtgtcc
     3241 atcgctgccg acacgatcgg cacggcgagg cggatgtcgg gtgtgaacca ggtcgacgtg
     3301 tcgctctcgt gcgggaggac gttcgactcg gcgggcatca acagcacgtc gtcgaacgtc
     3361 aggccgatcc ggccgaagcg ggcgttgaag gcggcgtcgc cgccaagttc atccgtgggg
     3421 agctgtgcca tacggcagca taactcccgc aaacgcgtgg gcaaggaacc cgatcgggtg
     3481 tgaactgcat cactccccga gttgtgcgca gcgcggtccg ccgagttcga acgtcgtgcg
     3541 catcactccg tcgacctcgt cgaacacctg gtcgctcatc gggaatgacg cgaccgtcgt
     3601 cggcagtgct ccgtcgatgc cgtcgaggga gggactcgac gcggaggcgt cgaaccggac
     3661 cacgatgagc tggtcgtcct gtggatcgcc ccgcccgggg cgctcgagtg tggtggtgcc
     3721 ctcgctgatc gggggcacgt cggcagagac ggccaggtcc acctcgggac gtccgtcgcc
     3781 catcgtcgac atcgggtcgt cggacagctt cgccccgagc ccggggccgc acgtgttcac
     3841 gatgtcgacg acgtagcggg ccggaacgat gctcgggccg ccggtgccgt cggcggccca
     3901 gtaggacacc ccggcgaacg cgacggcgat ggcgacgagt gccgctctct gcgccttctg
     3961 tcgccgaggc gacgggccga ccggcggcgc cggcgcattc gaggaatcgg ccagcgtggg
     4021 gtggtgtgcc atggcgtcga cggtatcggg gcactcggga tctgcatcat gaacggaccg
     4081 gtaggggtcc gttcatgatg cagatcctcc gatcagccga cgtcgggcgc ggacacgggc
     4141 aacgacgaga tcacggacgg cggacgggct ctgcaccgac tcgtatccga ggtacatcac
     4201 gtcccatgcc tcgacgccgg cggcgttgtc acggtggtcg tcgctcgcct cgttctcggc
     4261 accgaagtgg tgctcccggc tgtgcgcctc gatgccgagc atcgcgtcgg gcaccgccag
     4321 gtcgaaggtt gccacgtggc cgccctcggc gttgcggagc tcgtactgga tcacgaggcc
     4381 gtcgagctcg gggtggacga ggcacaactc gaccatccgc tcgaaccacg aaccccgcac
     4441 cgtcccgcgt tgctcgtgtc gatcgagcag gtccagcagc agcgccggcc cgaccacgtt
     4501 gggtcgcacc aggcgttccg ctgtctggcg cagccatcgg gggttcgcac ccgttcggat
     4561 cgagtggtcg aaggcgcgct ccacccgatc ccgctcgacg acgtcgccga ggtcgaccag
     4621 ggtccgggcg acgttcgtgc accggatccc gtcgaccacg acgaggtcgc agcgcggcag
     4681 cgaccgcacc cgatgtccga tcacccgtcg agtcggacca gacgtggcct ctcgatcgac
     4741 gcctcgatcg acggttcgtg ctcgaagccc tcgaggcggg cgagccgcgc tgcggaacga
     4801 tgggacgcca ccgcaccgtc acggccggcg gtcgcagcgg cgatctgctg gcgccacgtc
     4861 ggtccgccgt cggggacatg gcccacgaac acccctcgat ggggttcgac gatgaggccc
     4921 cgctcgatgg cggagcggag ctggcggcgg gtgagtgagt gggcagcagc ctgggcactg
     4981 gtgaagacgt tgttcggaga ggctgcgagc ttcgaaacaa cgcttacggc atcgtttcgc
     5041 atccagcgat gtcatcacgg acccctgaca ccggggcgct cggaccgatc ggtatcgcca
     5101 acggaccggt aggtgtcggt tggtgatgca gatcgagacc ggccggcgcg accggaccgg
     5161 agacctgggc aaagttgcgc caactcggtc cgtccggccc cgcccgggcc tacagtgctg
     5221 ggcggtgacg atgacggcga ccaccccctc ccccgctgtg cccgccgtgg gcgctgcggg
     5281 cgctgcgggt cccgaacgcc gcccgagcct gcttcgcacc gacaccccgg gccgcatgcg
     5341 ggtgtggtcg atcgccgtcg ccgtcgtcgc cgtgatcttc ggcatcgtcg gatcgaccgc
     5401 catcgccaac cgcgccaccg acctcggcga cgcccgggac gccgcagcgc agctcgaacg
     5461 cctcgaatcg gtgcgcacgg cggtggtcga ggccgactcc ctggcagcgt cggcgtatct
     5521 cgagggtggc ctcgagtcgc cggatcggcg ggccacctac gaggaccgtc tcgacgcggc
     5581 gcaggcgggc ctcgccgctg ccgccgcccg tgccaccgac gcggaggtcg aacagctcgg
     5641 cgggttggcc agcgccctca ccaccgccgc cggcctgatc gagcagtccc gggccaacag
     5701 ccgccagggg ttcccggtgg gcgcggccta ccagcgcaac gcgtcggcgc tcatccgctc
     5761 cgacgtgctc ccggagctcg actcgatcgc gacggcgacc gcggctcgga cctccgacga
     5821 ggtcgatcgt ggttcccgag cccaactcgt cctgtggggc cccgccctcg tggcgctcgg
     5881 gctcatcggg gccgcctccg tgtggctcac acggcgaacc cgtcgggtcg tcaacgtcgg
     5941 gttggcgatc gcagcgctcg cggtgctcgg cttcaccggc ttcgccagct ccgtgctggc
     6001 gtcatcgacg agcaacgccc gggccacgct cgacgggtcc tacgccggag cgaccgcgtt
     6061 cgcgcaggct cgaacagcag ccttcgacgc ccgctccaac gaggcgctga ccctcatcgc
     6121 tcgtggcaac ggtgctgcgt tcgagacccg ctggcaggaa caggctgcgc aggtcgcggc
     6181 cggcctcacc gacgcggcga aggccgactc ggtcaacggt cagaacgcga tcgacgggtt
     6241 cggcagctac ggcagcgagc atgtccaggt gcgcgagctc gacgacgccg gcgactacga
     6301 caccgccgtc gaacgggccc tcgccgtcgg caacggcggt gacgggttct tccagttcga
     6361 cgaggccagc cggaccgggc tcgagtcgtt gtcggccgac accgacgcgg cgctcggcga
     6421 ggcccgcgac agcgtgtcgg gccatcggtg gctcgtgctc gtggcggggt tcgttgc
//
LOCUS       DAOSOA010000181         6426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12170_length_6426_cov_6.798148, whole genome shotgun sequence.
ACCESSION   DAOSOA010000181 DAOSOA010000000
VERSION     DAOSOA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12170_length_6426_cov_6.798148"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PKY13_11195"
     CDS             complement(<1..473)
                     /locus_tag="PKY13_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80546.1"
                     /translation="MSAPIRPQRTVTTAAARLLAAAVLLSGALAASIAATPEAGAQAR
                     LVNALVRTFPARPDPGNDVTVLVGVTGCPPGPVTVEIYLTTSDGATQAATLMTRTAAT
                     TNLVLRTKAVLELPDAIEGWYGARVLCGNFRPPKVAMTNTLFEPTAKSVFVIATFG"
     gene            565..1044
                     /gene="tsaE"
                     /locus_tag="PKY13_11200"
     CDS             565..1044
                     /gene="tsaE"
                     /locus_tag="PKY13_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT80547.1"
                     /translation="MIQARTTSTEQTAALAAALAEMATSGDLIVLSGDLGAGKTAFAK
                     GFGAALGVTEPITSPTFTLVREYQGRLKMYHLDVYRLEQIEEALDLGLAELLDDDAVT
                     LVEWGEAILPALPPEYLEVRLELGEDDDERLLELVMIGRRWAARSVGIARAVGPWAA"
     gene            1088..1786
                     /gene="tsaB"
                     /locus_tag="PKY13_11205"
     CDS             1088..1786
                     /gene="tsaB"
                     /locus_tag="PKY13_11205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT80548.1"
                     /translation="MLILGIDTATSQAGCAIGGHEGVIASAQCAKGRRHAENLAPAIE
                     FICSQSQIELSEISVIAVDLGPGLFTGLRVGIATGKGLAHALGVPMIGVASLDLLAFA
                     VRFSSRLIVTAIDARRSELYYAFYRQVPGGIQRLGEHAVGSPDELVSQLSVRREEMLL
                     VGDGVHRYRELFETVPKAEIADAGLSFPSAASLVQLAHAQALREEFQPATSLEPIYLR
                     KPDAEINWVTRDRA"
     gene            1802..2320
                     /gene="rimI"
                     /locus_tag="PKY13_11210"
     CDS             1802..2320
                     /gene="rimI"
                     /locus_tag="PKY13_11210"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOT80549.1"
                     /translation="MVESGSQRVAVVPMRSRHLRGVVAIEQVTSPRPWSKDLFASELK
                     QASSRGFVAVRPDASVVGFACLMSTGFETHITNIAVHPSFRLQRVATMLMLRLVAETK
                     AWGLDDMTLEVRESNTGAQDLYRKFGFEQDGVRPRYYAETGEDALIMWVRGVGGEDYQ
                     RRIDAVIAAMRR"
     gene            2331..3344
                     /gene="tsaD"
                     /locus_tag="PKY13_11215"
     CDS             2331..3344
                     /gene="tsaD"
                     /locus_tag="PKY13_11215"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT80550.1"
                     /translation="MTTDFSPDAAILGIETSCDETAAALVCGGRVVLSSVVSSQVDLH
                     ARFGGVVPEIASRAHLDLLIPIVAESVVSSGVSESDIAAVGATYGPGLIGSLLVGVSA
                     AKALAMAWDVPFVAVNHLEAHLYASLLEDPDLEFPLVVLLVSGGHTLLVRMDGPGRYK
                     ILGQTLDDAAGEAFDKVARFLGLGYPGGPAIDRLAMSGDPTAINFPRAMLDDGYDFSF
                     SGLKTSVINWVRKHPDVDAADVAASFQQAVVDVLVTKARRAAVDVGARGLAIGGGVAA
                     NSQLRESFLDVCVGQGIRGFLPSREMCTDNAAMVAAAAWYKLTLDGPSPLDTGALPNL
                     ALS"
     gene            3476..3763
                     /gene="groES"
                     /locus_tag="PKY13_11220"
     CDS             3476..3763
                     /gene="groES"
                     /locus_tag="PKY13_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT80551.1"
                     /translation="MNLQPLEDRIVVRPAQAEATTASGLVIPDTAKEKPQQGEVLAVG
                     EGRFGDDNERIPMEVKVGDKVVYSKYGGTEITIDGEDLLILAARDVLAIAK"
     gene            3785..5416
                     /gene="groL"
                     /locus_tag="PKY13_11225"
     CDS             3785..5416
                     /gene="groL"
                     /locus_tag="PKY13_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT80552.1"
                     /translation="MAKILKFDDEARRALEAGVNKLADAVKVTLGPKGRNVVLDKKFG
                     APTITNDGVSIAREIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATVLAQALVKEG
                     LRNVAAGASPLGLKRGIEAAVAAAVESIQAQAKDVDDKSEIAQVAAISAADVKIGEVI
                     ADAIDKVGKDGVVTVEESNTFGMELDFVEGMQFDKGYLSPYFVTDAERQEAVLNDPYI
                     LFVNGKISSVQDLVPVLEKVMQGGKPLLIIAEDVDGEALATLVVNKIRGTFSSVAVKA
                     PGFGERRKAMLADMAILTGGQVISEEVGLKLDNTTIDLLGRARKVVITKDETTIVEGA
                     GNEDDVKGRVAQIKREVEDTDSDWDREKLQERLAKLSGGVAVVKVGAATEVELKEKKH
                     RIEDALSATRAAIEEGVVAGGGTALLRARASLQKTIDSLEGDEATGARAVYRALEAPA
                     RHIAENAGIEGAVAVQRTEAETGSVGLNAATGVFEDLLKAGVIDPAKVTRAALQNAAS
                     IAGLLLTTECLVADKPEEASAGGGMPDMGGMGGMM"
     gene            5757..6416
                     /locus_tag="PKY13_11230"
     CDS             5757..6416
                     /locus_tag="PKY13_11230"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOT80553.1"
                     /translation="MTSDPASLLILPSVTDRTRRSFYERTGKRAFDIVVATTMLACLA
                     PLMAAIWCLLRVTLGHDVVISQDRVGLDGETFGMFKFRTMRWSRRSAERPFQGVDRRQ
                     SHKVDHDPRHTPVGRVLRKLSLDELPQLVNVLLGSMSVVGPRPEMASVVDRLGERGHV
                     RHSVRPGMTGEWQVTGRQTGAPLHECFEADLPYLTRVTFRNDLGILIRTIAVVLQRGG
                     R"
BASE COUNT         1023 a         2168 c         2176 g         1059 t
ORIGIN      
        1 ccgaaggtgg cgatgacgaa cacgctcttc gccgtcggtt cgaagagcgt gttcgtcatc
       61 gccaccttcg gtgggcggaa gttgccgcac aggacacggg cgccgtacca cccctcgatc
      121 gcgtcgggga gctcgagcac cgccttcgtg cgcagcacca gattcgtcgt cgccgcagtc
      181 cgggtcatga gcgtcgccgc ctgggtcgcc ccgtcggatg tggtgaggta gatctcgacg
      241 gtcacggggc cggggggaca cccggtcacc cccacgagca cggtcacgtc gttgcccggg
      301 tcgggtcgtg ccgggaacgt ccgcacgagc gcgttcacga gtcgggcctg cgctcccgcc
      361 tcgggcgtcg cagcgatcga ggcggcgagg gcccccgaca gcaggacggc cgccgccagg
      421 aggcgcgccg ccgcggtggt gacggttcgc tgagggcgga tcggcgcgga catgtcgttc
      481 gtagggttgc agggcgcgcc ggagtgcgtc aacgcggcgg cgcccgacgc gggttccgtt
      541 cgtgaggccg tagtctggcg gtcgatgatt caagcgcgga cgacctccac cgagcagacc
      601 gctgccctcg cggcggcgct cgccgagatg gccacgtcgg gcgacctcat cgtgctgtcc
      661 ggcgatctgg gggccggcaa gaccgccttc gccaagggat tcggtgcggc gctcggcgtg
      721 accgagccga tcacgtcgcc gacgttcacg ctggtgcggg agtaccaggg ccgtctcaag
      781 atgtaccacc tcgatgtgta ccgtctcgag cagatcgagg aagcgctcga cctcgggctg
      841 gccgaattgc tcgacgacga cgccgtgacg ctggtcgaat ggggcgaggc gatcctgcct
      901 gccctgccgc ccgagtacct cgaagtgcga ctcgagctgg gcgaggacga cgacgagcgt
      961 ctcctcgaac tggtcatgat cggccgccgg tgggcggcgc ggtcggtcgg catcgcccgg
     1021 gcggtcggcc cgtgggccgc gtgaccgtgc ccgacctcat cgacgttccc agccgcgacg
     1081 gagtgacatg ctgatcctcg gaatcgacac cgcgacctcc caggcgggct gcgccatcgg
     1141 cggccacgaa ggggtcatcg cctcggcgca gtgcgcgaag ggtcggcgtc acgccgagaa
     1201 cctcgctcct gccatcgagt tcatctgctc gcagtcccag atcgagttgt cggagatcag
     1261 cgtgatcgcc gtggacctcg gtccgggcct cttcaccggg ctcagggtcg gcatcgccac
     1321 cggcaagggc cttgcccacg cgctcggggt gccgatgatc ggggttgcga gtctcgacct
     1381 gctcgcgttc gcggtgcggt tctcgtcgcg gctgatcgtg acagcgatcg atgcccgtcg
     1441 ctccgagctg tactacgcgt tctaccgcca ggtacccggc ggcatccaac ggctcggcga
     1501 gcacgcggtt ggttcacccg acgagctggt gtcgcagctg tcggtgcgtc gcgaggagat
     1561 gctgctcgtc ggcgacggtg tccaccggta ccgcgagctg ttcgagacgg tgccgaaggc
     1621 cgagatcgcc gacgccgggc tctcgtttcc atcggccgcc tcgctcgtgc aactggccca
     1681 cgcccaggcc cttcgcgagg agttccagcc ggcgacctcc ctcgagccga tctacctgcg
     1741 caagcccgac gccgagatca actgggtcac gcgggaccgg gcctgaccga cgtggccgac
     1801 cgtggttgag tccgggtccc agcgcgtcgc cgtggtgccg atgcgcagtc ggcacctccg
     1861 tggtgtcgtg gcgatcgaac aggtcacgag cccccggccg tggtcgaagg acctcttcgc
     1921 gtcggagctg aagcaggcgt cctcacgcgg cttcgtggcc gttcggcccg atgcctccgt
     1981 ggtcggcttc gcgtgcctca tgtcgaccgg cttcgagacc cacatcacca acatcgccgt
     2041 ccatccctcg ttccgcctcc agcgcgtggc gaccatgctc atgctccggc tggtcgcgga
     2101 gaccaaggcg tgggggctcg acgacatgac cctcgaggtc cgggagtcga acaccggagc
     2161 gcaggacctg taccgcaaat tcgggttcga gcaggacgga gtacgtcccc ggtactacgc
     2221 cgagacgggt gaggacgcgt tgatcatgtg ggtgcggggt gtcggcggcg aggactacca
     2281 gcgtcggatc gacgccgtga tcgcagccat gcgccggtag ggttggcgcg atgaccaccg
     2341 acttctcgcc agacgcagcg atcctcggca tcgagacgtc gtgcgacgag accgcggccg
     2401 ccctggtgtg cgggggtcgg gtggtgctgt cgtcggtggt gtcctcccag gtcgacctgc
     2461 acgcccggtt cggtggcgtg gttcccgaga tcgcgagtcg tgcgcacctc gatctcttga
     2521 tcccgatcgt ggccgaatcg gtcgtgtcgt cgggtgtgtc cgagtccgac atcgccgccg
     2581 tcggcgcgac gtacggtcca ggcctcatcg gttccctcct cgtcggcgtc agcgccgcca
     2641 aggccctggc gatggcgtgg gacgtgccgt tcgtcgccgt caaccacctc gaggcccacc
     2701 tctacgcgtc gctcctcgag gatccggacc tcgagttccc cctcgtcgtc ctgttggttt
     2761 cggggggcca cacgctgctc gtccgcatgg acggaccggg ccggtacaag atcctcgggc
     2821 agaccctcga cgacgccgcc ggggaggcgt tcgacaaggt cgcccggttc ctgggcctcg
     2881 gctatccggg cggccccgcg atcgaccggt tggcgatgtc gggcgacccg accgcgatca
     2941 acttcccccg ggcgatgctc gacgacggct acgacttcag cttctccggc ctcaagacct
     3001 ccgtcatcaa ctgggtgcgg aagcaccccg acgtcgatgc tgccgatgtc gcagcgtcct
     3061 tccagcaggc ggtcgtcgat gtgttggtca ccaaggcccg ccgggcagcg gtcgacgtgg
     3121 gcgctcgggg cctcgccatc ggcgggggag tcgccgccaa ctcccagctc cgcgagtcgt
     3181 tcctcgacgt gtgcgtcggc cagggcatcc gaggattcct gccgtcgcga gagatgtgca
     3241 cggacaacgc cgcgatggtc gccgcggctg cgtggtacaa gctgacgctc gacgggccga
     3301 gccccctcga taccggcgcg ctgcccaacc tcgccctgtc ctgagaccga tatcgggtgc
     3361 tgcttagcac tcggccatcg agagtgctag ctttggcact cgcgccaccc gagtgccaac
     3421 gccaggggtg cgcaatccac acacactcat gcgaaagcgc aacggaggca tctgcatgaa
     3481 cctgcagcca ctcgaagacc gtattgtcgt tcggcccgca caggccgagg cgaccaccgc
     3541 gagcggtctc gtcattcccg acaccgccaa ggaaaagccc cagcagggcg aggtgctcgc
     3601 cgtcggtgaa ggccgcttcg gtgacgacaa cgaacgcatc ccgatggagg tcaaggtcgg
     3661 cgacaaggtc gtctactcca agtacggcgg caccgagatc accatcgatg gcgaagacct
     3721 cctcatcctc gctgctcgcg acgtgctcgc gatcgccaag taatcaccac acaaggatcc
     3781 ccacatggca aagattctga agttcgacga cgaagctcgt cgtgccctcg aggccggcgt
     3841 caacaagctg gccgacgcgg tcaaggtcac gctcggcccc aagggccgca acgttgtcct
     3901 cgacaagaag ttcggcgcac cgaccatcac caacgacggt gtctccatcg ctcgtgagat
     3961 cgagctggag gaccctttcg agaacatggg cgcccagctc gtgaaggagg tcgccaccaa
     4021 gaccaacgac gttgccggtg acggcacgac gacggcgacg gtgctcgccc aggcactcgt
     4081 gaaggaaggc ctccgcaacg tcgccgccgg cgcgagcccc ctcggtctca agcgtggcat
     4141 cgaggcggca gtcgccgccg cggtcgagtc gatccaggcg caggccaagg acgtcgacga
     4201 caagagcgaa atcgctcagg tcgccgccat ctccgctgcc gacgtcaaga tcggcgaggt
     4261 catcgccgac gccatcgaca aggtgggcaa ggacggtgtg gtcaccgtcg aggagtccaa
     4321 cacgttcggc atggagctcg acttcgtcga gggcatgcag ttcgacaagg gctacctctc
     4381 gccctacttc gtgaccgacg ccgagcgtca ggaagcggtc ctcaacgatc cgtacatcct
     4441 gttcgtcaac ggcaagatca gctcggtcca ggacctcgtc ccggtgctcg agaaggtcat
     4501 gcagggcggc aagcccctcc tgatcatcgc cgaggatgtc gacggcgagg cgctcgccac
     4561 gctcgtcgtg aacaagatcc gtggcacgtt cagctcggtc gccgtcaagg ccccgggctt
     4621 cggtgagcgc cgcaaggcga tgctggccga catggccatc ctcaccggcg gtcaggtcat
     4681 cagcgaagag gtcggcctca agctcgacaa cacgacgatc gacctgctcg gtcgtgctcg
     4741 caaggtcgtc atcaccaagg acgagaccac catcgtcgag ggtgccggca acgaggacga
     4801 cgtcaagggc cgcgtggcgc agatcaagcg tgaggtcgag gacaccgact ccgattggga
     4861 tcgcgagaag ctccaggagc gcctcgcgaa gctctcgggc ggcgtcgccg tcgtcaaggt
     4921 cggcgctgcg accgaggtcg agctcaagga gaagaagcac cgcatcgagg acgccctctc
     4981 ggcgacccgc gctgccatcg aggagggtgt ggtcgccggt ggcggcacgg ccctcctccg
     5041 tgcccgtgcc tcgctccaga agacgatcga ctcgctcgag ggtgacgagg ccacgggcgc
     5101 ccgtgccgtg taccgcgcac tcgaggcccc ggcccggcac atcgccgaga acgccggcat
     5161 cgagggggcc gtcgcggtgc agcgcaccga ggcggagacc ggctcggttg gtctcaacgc
     5221 agcgaccggt gtgttcgagg acctgctcaa ggccggtgtc atcgatccgg ccaaggtgac
     5281 ccgcgccgcg ttgcagaacg cagcgtcgat cgccggcctg ctcctcacca cggagtgcct
     5341 ggtcgccgac aagccggagg aagcctccgc cggcggcggc atgcccgaca tgggtggcat
     5401 gggcggcatg atgtaatgac cccccgccgg ctcgtccggc agggttgaat ctgtaccgaa
     5461 tgtgcttcgg ggccggctcc actgggggcc ggccccgggg cgttttcagc gccggttcga
     5521 tgcaccacgt gacgttccac gcgcgtgggt taaatcaccc agcaacagtt ctcacctggg
     5581 aatttctcga caaaccggta ctattgggac caacggctca ttgaatccca ggcggggctc
     5641 tgttgacgtt gacacgctcg gtgtgcttgg aggtaccccc tccagcggcg agttcagagt
     5701 ggattatgta tcgattgcag gcagacgacg agcgagtggg gcgggggatc gatgccgtga
     5761 cgtcggatcc ggcgtcgctc ctgatcctcc cttcggtcac cgaccggacg cgtcgatcct
     5821 tctacgagcg gacgggcaag cgagcgttcg acatcgtcgt cgcgaccacg atgctggcat
     5881 gtctggcgcc gctgatggct gcgatctggt gcctgctccg ggtgacgctc ggccacgatg
     5941 tggtgatctc ccaggaccgc gtcggtctcg acggcgagac cttcggcatg ttcaagttcc
     6001 gcacgatgcg gtggagccgt cgctccgccg aacggccgtt ccagggcgtg gaccggcgtc
     6061 aatcccacaa ggtcgaccac gatccccggc acacccccgt gggtcgtgtg ctccgcaagc
     6121 tcagcctcga tgagcttccg cagctcgtga acgtcctgct gggttccatg tccgtcgtcg
     6181 gtccccgtcc cgagatggcg tcggtggtcg atcgacttgg cgagcgcggc cacgtgcggc
     6241 actccgtccg cccgggcatg accggcgagt ggcaggtgac gggtcgacag accggtgccc
     6301 cgctccacga gtgtttcgag gccgaccttc cctacctgac ccgagtcact tttcgtaatg
     6361 atctcggcat actgatccgg acgattgctg tggtgttgca gcgaggaggc cggtgatgac
     6421 cagttc
//
LOCUS       DAOSOA010000182         6374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12299_length_6374_cov_6.965659, whole genome shotgun sequence.
ACCESSION   DAOSOA010000182 DAOSOA010000000
VERSION     DAOSOA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12299_length_6374_cov_6.965659"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..2356
                     /gene="scpA"
                     /locus_tag="PKY13_11235"
     CDS             167..2356
                     /gene="scpA"
                     /locus_tag="PKY13_11235"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOT80554.1"
                     /translation="MSAQLPDFTQIAAPTPSAGDADRWRSGVESATGLELSKLAWETP
                     EGIDVAPLYTAADVSGLDFLDTYPGIAPFLRGPYPTMYVNQPWTVRQYAGFSTAEDSN
                     AFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRVLFDGIP
                     LDQMSVSMTMNGAVLPVLALYIVAAEEQGVGPEKLAGTIQNDILKEFMVRNTYIYPPA
                     PSMRVISDIFAYTSKHMPKYNSISISGYHMQEAGATADLELAYTLADGVEYLRAGIDA
                     GLPVDAFAPRLSFFWAIGMNFFMEVAKLRAARLLWARLVREFQPSNDKSLSLRTHSQT
                     SGWSLTAQDVFNNVVRTCVEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLF
                     LQQESNTTRVIDPWGGSYYVERLTYELARKAWDHIAEIDGLGGMAKAIEAGIPKLRIE
                     EAAARTQARIDSGRQSVIGVNKFRPNGDEKIDVLKVDNATVRAGQIAKLERLRAERNS
                     DAVTSALEALTNAAGSGDGNLLELAVDAARKMATVGEISEALEKVYGRHRAEIRSISG
                     VYRSEMGADNETVERVRRKVEAFEAADGRRPRILLAKMGQDGHDRGQKVVASAFADLG
                     FVVDIGPLFTTPQEAAQQAVDADVHIVGPSSLAAGHLTLVPELVQALADLGRPDIMIV
                     VGGVIPPQDFDELRAAGAAAIFPPGTVIAAAADDLLDQLADRLGYSL"
     gene            complement(2271..3590)
                     /locus_tag="PKY13_11240"
     CDS             complement(2271..3590)
                     /locus_tag="PKY13_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT80555.1"
                     /translation="MKRRLVISFLSVTAVVLILLAVPMGYLLQRVASDESKAQLDQQA
                     SYLLDQAVDPTTSILRVNASGLDRLLPPNSSAVLKLAGGSTVEVRELPAGDLISATAS
                     DVNGSSATVYASANPVRDRVRQPLIILAGLGVAGLLTTWLLAQAEARRLGQPLRELAA
                     TAARLGSGDFSVIAPRSGVPELDSLATTLDASAARIDALVRAERSFNSDAGHQLRSAL
                     TGLRLRLEELAHTDDPDTAAEVQAAIEQADRLSATIDALLRLSRTGRAGVATRFDLRT
                     LTASHVDDVGVALRRQRRRIDVVPGPPLTIEAAPGAVGQALDVVLWNAARHGRGRVTL
                     SLRDADGWAELTVSDEGSIDPATLSDLFVEPPRPDTHGIGLPLARRIIESEGGRLDVA
                     ETDPTTFRIRLPLPSSGDDVRASSPTDRPVDRAGRLRLRRSPSRAGR"
     gene            complement(3587..4267)
                     /locus_tag="PKY13_11245"
     CDS             complement(3587..4267)
                     /locus_tag="PKY13_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT80556.1"
                     /translation="MHRLLLVEDDAAIAQPLQRALSREDFEVVHVALGADAIAAALAG
                     GFDLVVLDLTLPDIDGLDVCRAIRAEDSGLPIIMLTARSDELDLVVGFDAGADDYMAK
                     PFSMAELTARVRARLRHTVATESVIEAQGIRLETDAHRVWNGEEELHLTPKEFELLSL
                     LVSEAGRVVTRQRILSEVWDDDFYATSRSLDVHVSALRRKLGDHPDQPRYVTTVRGVG
                     FRFETGAP"
     gene            4262..4438
                     /locus_tag="PKY13_11250"
     CDS             4262..4438
                     /locus_tag="PKY13_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80557.1"
                     /translation="MHADERKRRSSECADPIADEAITDRLYRFFAVETAILRGLFNEN
                     CRWGPAWSPEVSPR"
     gene            4542..5549
                     /gene="meaB"
                     /locus_tag="PKY13_11255"
     CDS             4542..5549
                     /gene="meaB"
                     /locus_tag="PKY13_11255"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOT80558.1"
                     /translation="MRPQLTAAELADGVLAGRRTVLAQAITMTESRRADHRRLAAEVL
                     DRVIGSTGSSIRVGITGVPGVGKSTFIDQFGTNLTRAGHRIAVLAVDPSSQRTGGSIL
                     GDKTRMERLAVDPDAFIRPSPAGATLGGVTRTTREAMLLCEAAGFDVILVETVGVGQS
                     ETIVADMVDFFLVLMLSGAGDELQGIKKGVLELADMVAVNKSDGDNADRARRAAMDYR
                     HALHLMAQPTPTWSPPVTTCSGLANEGLDEIWDQIELHRSKLGATGELAAKRQQQQVH
                     WMWSMINDRLLDEFRSHDAVRDLLGEVESKVRDGEVAATVAADGLVDRFLTPPEQLRP
                     E"
     gene            5639..6358
                     /locus_tag="PKY13_11260"
     CDS             5639..6358
                     /locus_tag="PKY13_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOT80559.1"
                     /translation="MAGLVVVGLVLGVLIATWWSITPVSRTPSPRVAANAGSTQSPDG
                     WTDRGWAVVDDEPWRAAVVRITTDRCGVEIRGSGVIVDGIVLTNRHVIDGAASVLLTT
                     ADGTTHPVRSIGVSSEVDLARLAAGGLPRGLSLAEGPSALGDGYVLAGFPAGHDFSSR
                     AAAVAEVDRGWGFPDPERRLRLDRPVVPGESGSAVIDRRGDVAALVYARANTDGHGLA
                     IDAVELDEANRRTDNQSFTDC"
BASE COUNT          984 a         2154 c         2243 g          993 t
ORIGIN      
        1 cggcgacggc cctgcgggac gcaggtgcgt cccggatcta cctggccggc gcacccggcg
       61 aacgccgcga ggagctgctg gccgccggcg tcgacgagtt catccacgtc ggcgtcgacg
      121 tgctcgcgtc cctccgaacc gcccacgacc tcgagggggt ggcgcgatga gcgcccaact
      181 gcccgacttc acccagatcg ccgccccgac tccgtctgcc ggcgatgccg accggtggcg
      241 ttccggcgtc gagtccgcga ccgggctcga gctctcgaag ctcgcgtggg agacgcccga
      301 gggcatcgac gtggcgccgt tgtacaccgc cgccgacgtg tcgggcctcg acttcctcga
      361 cacctacccc ggcatcgccc cgttcctccg tgggccgtac cccacgatgt acgtgaacca
      421 accatggacg gtgcggcagt acgcagggtt ctcgaccgcc gaggactcca acgcgttcta
      481 ccggcgcaat ctcgccgccg gccagaaggg cctgtcggtc gcgttcgacc tggcgaccca
      541 ccgcggctac gactccgacc acccccgtgt tgccggtgac gtcggtatgg ccggcgtggc
      601 gatcgattcc atctacgaca tgcgggtgct gttcgacggc atccccctcg accagatgtc
      661 ggtgtcgatg acgatgaacg gtgcggtgct gcccgtgctc gctctctaca tcgtggcggc
      721 cgaggagcag ggtgtcgggc ccgagaagct cgccgggacc atccagaacg acatcctcaa
      781 ggagttcatg gtccggaaca cctacatcta cccgccggcg ccgtccatgc gggtgatctc
      841 ggacatcttc gcctacacca gcaagcacat gccgaagtac aactcgatct cgatctccgg
      901 ctatcacatg caggaggcgg gagcgacggc cgacctcgag ctggcgtaca cgctggccga
      961 cggcgtcgag tacctccggg ccggcatcga tgccggtctt cccgtcgacg cgttcgcccc
     1021 gcggctcagc ttcttctggg ccatcgggat gaacttcttc atggaggtgg ccaagctcag
     1081 ggccgctcgt ctgctgtggg cgcgcctcgt gcgcgagttc cagccgagca acgacaagtc
     1141 gctctcgctg cgcacccaca gccagacctc ggggtggtcg ctcaccgccc aggacgtgtt
     1201 caacaacgtc gtccgcacct gtgtcgaggc gatggccgcc acccaggggc acacccagtc
     1261 gctccacacc aacgcgctgg acgaggccct cgcgctgccg accgacttct cggcccggat
     1321 cgcccgcaac acccagctct tcctgcaaca ggagtcgaac acaacacgcg tcatcgaccc
     1381 gtggggcggc agctactacg tcgagcgcct cacctacgaa ctcgcccgca aggcgtggga
     1441 ccacatcgcc gagatcgacg gtctcggcgg catggccaag gcgatcgaag cgggcatccc
     1501 caaactgcgc atcgaagagg cggccgctcg cacccaggca cgaatcgact cgggccgcca
     1561 gtcggtcatc ggcgtcaaca agttccggcc caacggtgac gagaagatcg acgtgctcaa
     1621 ggtcgacaac gccacggtcc gcgccggtca gatcgcaaag ctcgagcggc tgcgtgccga
     1681 gcgcaactcc gacgccgtga cctcggcgct cgaagccctc accaacgccg ccggttcggg
     1741 cgacggcaac ctgctcgagc tcgcggtcga cgccgcccgg aagatggcca ccgtcggcga
     1801 gatcagcgag gccctcgaga aggtctacgg tcggcatcgg gccgagatcc ggtcgatctc
     1861 gggtgtgtat cggagcgaga tgggtgcaga caacgagacg gtcgaacggg tccgtcggaa
     1921 ggtcgaggcc ttcgaggcgg ccgatggccg gcggccccgg atcctgctcg ccaagatggg
     1981 ccaggacggc cacgaccgcg gtcagaaggt cgtcgcgtcg gcgttcgccg acctcgggtt
     2041 cgtcgtcgac atcggtccgt tgttcaccac gcctcaggaa gccgcgcaac aggcggtcga
     2101 cgccgacgtg cacatcgtcg gtccgtcctc gttggcggcc ggccacctga ccctggtgcc
     2161 cgagctggtg caggcactgg ccgatctcgg gcgccccgac atcatgatcg tggtcggcgg
     2221 cgtgatcccc ccgcaggact tcgacgagct ccgcgccgcc ggcgcggccg ctatcttccc
     2281 gcccgggacg gtgatcgccg cagccgcaga cgacctgctc gatcaactgg ccgatcggtt
     2341 gggctactcg ctctgacatc gtcgccgctc gacgggagcg gcaaccggat ccggaaggtg
     2401 gtcgggtcgg tctcggccac gtcgagccgc ccgccctcgg actcgatgat gcgccgcgcc
     2461 agcgggagtc cgatgccgtg ggtgtcgggc cgcgggggtt ccacgaacag gtcactgagt
     2521 gtcgccggat cgatgctgcc ttcatcggag acggtgagct cggcccagcc gtcggcgtcg
     2581 cggagcgaca gtgtcacccg acctctgccg tgccgtgccg cgttccacag caccacgtcc
     2641 aacgcctggc cgacggcgcc gggtgccgcc tcgatcgtga gcggcggacc gggaaccacg
     2701 tcgatccggc gccgctgacg tcgcagcgcc accccgacgt cgtcgacgtg gctcgcggtg
     2761 agggtccgca ggtcgaacct ggtggccacg ccggcccgac cggtccgcga caggcgcagc
     2821 agtgcatcga tggtcgcgga gaggcggtcc gcctgttcga tcgcggcctg tacctcggcg
     2881 gccgtgtcgg gatcgtcggt gtgggcgagt tcctcgagcc ggagacgaag gccggtcaga
     2941 gcggaccgca gctggtgccc agcgtcgctg ttgaaactgc gttcggcgcg cacgagcgcg
     3001 tcgatgcgcg ccgccgacgc gtcgagcgtc gtggcgaggc tgtcgagctc ggggacgccc
     3061 gagcgcggcg cgatcaccga gaagtcaccg gaaccgaggc gggcggccgt tgctgcaagt
     3121 tcgcggaggg gctggccgag gcgtcgggct tcggcctgcg cgagcaacca cgtggtgaga
     3181 aggccggcga cgccgaggcc ggcgaggatg atcagcggtt ggcggacccg gtcgcgtacg
     3241 ggattggcac tcgcgtagac ggtcgctgac gacccgttga cgtcggaggc ggtggccgag
     3301 atcaggtcgc cggcgggaag ctcgcgcact tcgacggtgc tcccgccggc gagtttgagc
     3361 accgcgctgc tgttgggcgg gagcaggcga tcgagcccgg acgcgttgac gcggaggatc
     3421 gatgtggtcg ggtcgaccgc ctggtcgagg aggtaggacg cctgctggtc gagctgcgcc
     3481 ttggactcgt cgctggcgac gcgctggagg aggtagccca tcgggacggc gagcaggatg
     3541 aggacgacgg cggtcacgct caggaacgag atgaccaacc gtcgtttcac ggtgccccgg
     3601 tctcgaagcg gaagccgacg ccgcgcacgg tggtgacgta gcgcggctgg tcgggatggt
     3661 caccgagctt gcggcggagg gccgacacgt gcacgtcgag agatcggctg gtggcgtaga
     3721 agtcgtcgtc ccacacctcg ctcaggatgc gctgtcgggt gacgactcgg ccggcctcgg
     3781 agacgaggag cgacagcagc tcgaactcct tgggggtgag gtggagctcc tcttcgccgt
     3841 tccacacacg gtgggcgtcg gtctcgagtc ggatgccctg cgcctcgatg acgctctcgg
     3901 tggcgaccgt gtggcggagt cgagccctga cccgggccgt cagctccgcc atggagaagg
     3961 gcttggccat gtagtcgtcg gcgccggcgt cgaagccgac gacgaggtcg agctcgtcac
     4021 tccgggccgt gagcatgatg atcggtaggc cggagtcctc ggcgcggatg gcccggcaca
     4081 cgtcgagtcc gtcgatgtcg gggagcgtga ggtccagcac aacgaggtcg aacccgcccg
     4141 cgagggcagc agcgatcgcg tcagcaccca acgccacgtg gacgacctcg aagtcctctc
     4201 gactcaaggc gcgttgcagt ggctgggcga tcgcggcgtc gtcctcgacg agaagtaggc
     4261 ggtgcacgcc gacgagcgta aacgtcgcag ctccgagtgt gccgatccga tcgccgatga
     4321 ggcgatcacc gatcgccttt accgtttctt cgcggtcgag acggcgattc ttcgcggcct
     4381 gttcaacgag aattgtcgtt ggggtcccgc gtggtctcca gaggtttctc ccagatgagg
     4441 ttctcccagt ctcagggcgc ccttcggggc gcccacctct ttttcccggc ggcgtcgagc
     4501 ggtgaccgca gtccggatcg gatctgtcta cgatgccgac gatgcgaccc cagctgacag
     4561 cggccgagct ggccgacgga gtgctcgcgg gtcgacggac cgtcctggcc caggcgatca
     4621 cgatgaccga gagccgccgc gccgatcatc ggcggctggc cgccgaggtg ctcgaccgtg
     4681 tgatcgggtc gaccggctcc tcgatccggg tcggcatcac cggtgtaccg ggcgtcggca
     4741 agtcgacgtt catcgatcag ttcgggacca acctcacgcg ggccggacac cgcattgccg
     4801 tgctcgccgt cgacccgtcg agccaacgca ccggcggctc gatcctgggt gacaagaccc
     4861 gtatggagcg cctcgccgtc gaccccgacg cgttcatccg gccgtcacct gcgggcgcca
     4921 cgttgggtgg ggtcacccgg acgacgcgcg aagccatgct cctgtgcgag gccgccgggt
     4981 tcgacgtgat cctggtcgag acggtgggtg tgggccagtc cgaaacgatc gtcgcggaca
     5041 tggtcgactt cttcctggtg ctcatgctct cgggggccgg cgacgagctg caggggatca
     5101 agaagggggt gctcgagctc gccgacatgg tcgcggtcaa caagtccgac ggtgacaatg
     5161 ccgaccgggc ccgtcgggcg gccatggact atcggcatgc cctgcacctc atggcgcaac
     5221 cgacgccgac gtggtcgccg cccgtcacca cctgcagcgg cctggccaac gagggcctcg
     5281 acgagatctg ggaccagatc gagctgcacc gctcgaagct cggcgcgacg ggggagctgg
     5341 cggccaagcg gcagcagcaa caggtgcact ggatgtggtc gatgatcaac gaccggttgc
     5401 tggacgagtt ccgatcccac gatgcggtcc gcgacctcct cggcgaggtc gaatcgaagg
     5461 tccgcgacgg tgaggtcgca gcgacggtgg ctgcggacgg cctcgtcgat cgctttctga
     5521 cgccgccgga gcagctccga ccggagtgaa acctcctaaa aagacttgaa atagcacgac
     5581 atgctgtgat cacactgctg gcgtgcgtgg aggccgaaca tcgggatcgt cggagccggt
     5641 ggccgggctc gtggtcgttg ggctcgtact cggtgtgctc atcgcgacct ggtggtcgat
     5701 cacgcccgtg tcccgcacgc cgtcgcctcg ggtcgcggcc aacgccgggt cgacccagtc
     5761 accggacggc tggaccgatc gtggctgggc cgtcgtcgac gacgagccgt ggcgcgcggc
     5821 ggtggtgcgc atcacgaccg accggtgtgg tgtcgagatc cgcgggagcg gcgtcatcgt
     5881 cgacgggatc gtcctgacca accggcatgt gatcgacggc gccgcgtcgg tgctgctgac
     5941 gacggcggat ggcaccaccc accccgttcg gagcatcggg gtgtcgtcgg aggtggatct
     6001 cgcccggctc gccgccggtg gtctcccgcg cggtctgtcg cttgccgagg ggccgtcagc
     6061 cctcggtgac gggtatgtgc ttgccgggtt cccggctggt cacgacttct cgagccgtgc
     6121 cgccgccgtc gccgaggtcg accgggggtg ggggttcccc gacccggaac gtcgtctccg
     6181 gctcgaccgt ccggtcgttc ccggcgaatc gggaagcgcc gtgatcgacc gccgcggcga
     6241 tgtcgcagcg ctcgtctatg cgcgggcgaa caccgacggg cacggcctgg cgatcgatgc
     6301 cgtggagctg gacgaggcca atcgacggac ggacaaccaa tcgttcaccg actgctgacg
     6361 gatcactccg tgga
//
LOCUS       DAOSOA010000183         6353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12353_length_6353_cov_5.712290, whole genome shotgun sequence.
ACCESSION   DAOSOA010000183 DAOSOA010000000
VERSION     DAOSOA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12353_length_6353_cov_5.712290"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="PKY13_11265"
     CDS             complement(<1..1781)
                     /locus_tag="PKY13_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT80560.1"
                     /translation="MILVDLVRVGASRPGKPLFADLSLTINTGDRLGIVGINGCGKST
                     LMRVMSGADLPESGTVRMGSGVRVAVLDQEPRLTGGTVHEAAGAGWESDAVLDRLGMG
                     AHLDTPVDRLSGGQAKRVALARALTAESDLLILDEPTNHLDIDAIAWLEERLAAYSGG
                     LVMVTHDRHVLDRVTNRVLELDRGQAFLHDGGYDVYLEGKADRAEADAKAESVRKNLA
                     RTELAWLRRGAPARTSKPKARIATATALVEGKARAEARTGDLPLHFGTPRLGDQVVEL
                     HGIGHRYADDRPWLFRDVELLLDPRERLGIVGRNGTGKSTLLEIIAGRLEPAAGTIKL
                     GQTATVGYFDQRGRDLDPTARVRDVVAGPHRTPDHTDKALMEAFWFDGDAQYAPVGLL
                     SGGERRRLQLLVTLAEKPNVLLLDEPTNDLDLDTLRALEEFLDEWPGALLVVSHDRAF
                     LERTVADVIVLDGTGFAGRKPGGYAAYETERRAAPRRRGSVADATPRPDSAPKPSRPA
                     APSAGAADPDAKVRSPSTLRHRFRAVEREQATLQARHDELNAELVASSADHVRLAELA
                     VELQSVDAELSSIEEEWLELAAEAEERG"
     gene            1915..2574
                     /locus_tag="PKY13_11270"
     CDS             1915..2574
                     /locus_tag="PKY13_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HOT80561.1"
                     /translation="MTNSTAPATHTTDSATVDPRELFARAAAATRGLLAGLTPADHGR
                     STPCDDMDVAELGSHLLMVLERVVALGNGTDAMSLPDTSGVPEAGWTAAWDEAYSRAE
                     AAWGDDAKLDQPMFLPWVQGPGSQLLIGYVAELTVHTWDLGRALDVVVAWDDDVVEAA
                     WASYRAIFPTADRTERFAAARAAMPPQFRDNPPPFGNAVEIADDAPLIERVVAWTGRR
                     V"
     gene            complement(2599..4311)
                     /locus_tag="PKY13_11275"
     CDS             complement(2599..4311)
                     /locus_tag="PKY13_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT80562.1"
                     /translation="MHDLVIRNANIVDGSGAPAFTGDIAVDGDRIVAVGVVEGEGRRE
                     IDAAGNLVTPGWVDIHTHYDGQVTWDPEVSPSGWHGVTTIVMGNCGVGFAPADPARRE
                     WLIQLMEGVEDIPGAALDEGMSWNWETFPEYLDEVDSMGRVLDVAALVPHGAVRAYVM
                     GNQAAVHDQATPEQIDAMTTIVREAIEAGAIGFSTTRTLLHRAKDGELAAGTNADAAE
                     LIGIGRALGEADAGVFSVASDMFDPVSEIAWMTEVSRESGRPVTFACLQSPIQPDNWR
                     QLIELADGAAENGAHIVPQVAGRPACVMLGFESSANPFMFHQGYKEVADLPLAERVAH
                     LRKPEVRRAILDEQVELRGLQAIVMQSFHRLYPLGDPPEYEPGPDRSVLAIAEREGRS
                     PAEVAYDLMLENDGHALLYFPLIGYAAGDFDALEEMLHHPTTVLGLGDGGAHCGVLCD
                     AGLPTYMLAHWVRDRSRGGRLTIEEAVHHQTRRTAALYGFEDRGLLAPGYLADINVID
                     FEGLTVPAPEMVYDLPAGGRRLIQRAEGYLATIKSGVVVRDHDEPTGERPGRLLRGHQ
                     PAPA"
     assembly_gap    4364..4463
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4464..4828)
                     /locus_tag="PKY13_11280"
     CDS             complement(<4464..4828)
                     /locus_tag="PKY13_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80563.1"
                     /translation="MTTIDFQLFDADNHYYEAPDAFTRHIEPKFAKRGMQWVTVGGKT
                     RLMVDGRLNRFIPNPLFDPVAKPGVLDDYFRGKSGSDDIRSAFGELEPINPGYRDPAA
                     RVKIMDTQNMEACFMFPTLA"
     gene            4923..5717
                     /locus_tag="PKY13_11285"
     CDS             4923..5717
                     /locus_tag="PKY13_11285"
                     /inference="COORDINATES: protein motif:HMM:NF005873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT80564.1"
                     /translation="MITDHFLVTDQVAIVTGAGRGIGRATALALAEAGADLVISARTA
                     DQLEEVKQQVARLGRRAVCVTADLSDLATLPGLVETATGQFGRLDIVVNNVGGSFPRP
                     LLETSAAMFEKAFHFNVTTALELSKAAIPAMLASDPAGGSIVNISSAIGRMTDRGFAA
                     YGTAKAALTHLTRLMATECAPRIRVNAIAVGSTATSALDMVLTDDGLRTAMEDNTPLR
                     RLGDPLDIALGVLYLSSPAGSYLTGKILEIDGGLNAPNLLLGLPDL"
     gene            complement(5726..6121)
                     /locus_tag="PKY13_11290"
     CDS             complement(5726..6121)
                     /locus_tag="PKY13_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80565.1"
                     /translation="MKRVALGVAVVVTAVAGGTSACSGAEWAAPVEPPDGFAITAPVD
                     GEPCGEYLLVPDSSIDDPYDALAQWVDQVTDSPGATHRDRPEDLRTAPDGTYDGGITG
                     WNESDDGGGGFPVRVHFDGSRIVAEFCLN"
     gene            complement(6118..>6353)
                     /locus_tag="PKY13_11295"
     CDS             complement(6118..>6353)
                     /locus_tag="PKY13_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80566.1"
                     /translation="FVDLAVGRDRIDVGETEDDVVGCLKGNRIDVVTPVAAKDLLARQ
                     TTAKAEQSRIEGLINAQSQAATQSSTTIATVGG"
BASE COUNT          908 a         2216 c         2170 g          959 t
ORIGIN      
        1 ccccgctcct cggcctcggc cgccagctcg agccattcct cctcgatgct cgacagctcg
       61 gcgtcgaccg actgcagctc gacggcaagc tccgcgagcc gcacgtgatc ggccgacgac
      121 gccacgagtt cggcgttcag ctcgtcatgg cgcgcctgca gcgtcgcctg ttcgcgctcc
      181 acggcgcgga accgatggcg cagcgtggag gggctccgga ccttcgcgtc cggatcggct
      241 gcgccggcgc tcggcgccgc cgggcgcgac ggcttcgggg cggagtcggg acgcggggtc
      301 gcatcggcga ccgaaccccg acgtcgcggc gcggcccgcc gctcggtctc gtaggccgca
      361 tatccaccgg gcttgcggcc ggcgaacccg gtgccgtcga gcacgatgac gtcggccacg
      421 gtccgttcga ggaacgcacg gtcgtggctc acgacgagca gcgcccccgg ccactcgtcg
      481 aggaactcct cgagcgcccg cagcgtgtcg aggtcgaggt cgttggtcgg ctcgtcgagg
      541 aggagcacgt tcggcttctc ggcgagcgtg acgaggagtt ggagacgacg acgctcgccg
      601 cccgacaaca ggccgaccgg cgcgtactgg gcatcgccgt cgaaccagaa cgcttccatg
      661 agcgccttgt cggtgtggtc gggagttcgg tgcggaccgg cgacgacgtc gcgcacgcgg
      721 gcggtcgggt cgaggtcacg accgcgctgg tcgaagtagc cgaccgtcgc cgtctggccg
      781 agcttgatcg tgccggcggc cggctcgagg cggccggcga tgatctcgag cagcgtcgac
      841 ttgccggttc cgttgcggcc gacgatcccg agccgctcgc gagggtccaa caggagctcg
      901 acgtcgcgga acagccaggg ccggtcgtcg gcgtagcggt ggccgatgcc gtgcagttca
      961 acgacctgat cgccgaggcg cggcgtgccg aagtgaagcg ggaggtctcc ggttcgggcc
     1021 tccgcccgcg ccttgccctc gaccagcgcc gtcgcggtgg cgatgcgggc cttcggcttg
     1081 gaggtccgcg ccggggcgcc gcgtcgcaac catgccagct cggtgcgcgc gagattcttg
     1141 cggacggact cggccttggc gtcggcctcg gcacgatcgg ccttgccctc gagatacacg
     1201 tcgtagccgc cgtcgtggag aaaggcctgg ccccggtcga gttcgagcac gcggttcgtc
     1261 acccggtcga gcacgtgacg gtcgtgggtg accatcacga ggccgccgga gtacgcggcg
     1321 agtcgctcct cgagccacgc gatcgcgtcg atgtcgaggt ggttggtcgg ctcgtcgagg
     1381 atcaagaggt cggactcggc ggtgagcgcc cgcgccaggg cgacccgctt ggcctggcca
     1441 ccggagaggc gatcgacggg ggtgtcgaga tgggcgccca tgccgagacg gtcgaggacg
     1501 gcatcggact cccagccggc gccggctgcc tcgtgaaccg tgccaccggt gagccggggt
     1561 tcctggtcga gcacggcgac gcgcacgccg ctacccatcc gcaccgtgcc gctctcgggc
     1621 aggtcggctc cgctcatcac gcgcatcaac gtcgacttgc cgcacccgtt gatgccgacg
     1681 atgccgagac ggtcgccggt gttgatggtg agcgagaggt cggcgaacag cggcttgccg
     1741 ggacgggagg cgcccacacg gacgaggtcg acgaggatca tcgagtcgcg aggctacgcg
     1801 tccggccacc ggcggcggtc cgcctgtctg ccgggaccac cgcgccgcgt tcgttcgaac
     1861 cttctcgcaa atgtggccac aaggtggcca catttgggtc gcacactgca ctccatgacc
     1921 aacagcaccg ctccagccac ccacaccacc gacagcgcca ccgtcgaccc tcgcgagctc
     1981 ttcgcccggg ccgcggctgc gacccggggc ctgcttgccg ggttgacgcc cgccgaccac
     2041 ggtcgctcga cgccgtgcga cgatatggac gtcgccgagc tcgggagcca tctgctgatg
     2101 gtcctcgaac gtgttgtcgc cctcgggaac ggcaccgacg ccatgtccct ccccgacacg
     2161 tcgggcgtcc ccgaggcggg atggaccgcc gcgtgggacg aggcgtactc acgcgccgag
     2221 gcggcctggg gcgacgacgc gaagctcgac caaccgatgt tcctcccctg ggtccagggc
     2281 cccgggagcc agctcctcat cggctacgtc gccgaactca ccgtgcacac ctgggacctc
     2341 ggccgggcgc tcgacgtcgt cgtcgcctgg gacgacgacg tcgtagaggc agcgtgggcc
     2401 tcgtaccgcg ccatcttccc aaccgccgac cgaaccgagc ggttcgccgc ggctcgcgcc
     2461 gccatgcccc cgcagttccg ggacaacccg ccgccattcg gcaacgccgt cgagatcgcc
     2521 gatgacgcgc cgctcatcga gcgggtcgtc gcgtggaccg gccgacgcgt ctgatcgccg
     2581 cgacggtggc cgacgccgtc aggccggagc cggctggtgc ccgcggagca gccggcccgg
     2641 gcgctcgccc gtcggctcgt cgtggtcacg cacgacgacc ccggacttga tcgtggcgag
     2701 gtaaccctcg gcccgctgga tgaggcgacg accaccggcg ggcaggtcgt agaccatctc
     2761 gggggcgggc accgtcaggc cctcgaagtc gatcacgttg atgtcggcga ggtagccggg
     2821 ggcgagcagc ccccggtcct cgaatccgta caacgcggcg gttcggcggg tctggtggtg
     2881 gaccgcttcc tcgatcgtca accggccgcc acgactgcgg tcgcgtaccc agtgggcgag
     2941 catgtacgtc ggcaggccgg cgtcgcagag caccccgcag tgagccccgc cgtcgccgag
     3001 gccgagcacc gtcgtcggat ggtggagcat ctcctcgaga gcgtcgaaat cgccggcggc
     3061 atagccgatg agcgggaagt acagcagcgc gtggccgtcg ttctcgagca tgaggtcgta
     3121 cgcgacctcc gccggcgagc ggccttcgcg ctcggcgatc gcgagcacac tgcggtcggg
     3181 gccgggctcg tactcgggcg ggtcgccgag cgggtacagg cggtggaacg actgcatcac
     3241 gatggcctgg agaccacgca gctcgacctg ttcgtcgagg atcgcccggc gcacctcggg
     3301 tttgcggagg tgcgccactc gctcggcgag cgggaggtcg gcgacctcct tgtagccctg
     3361 gtggaacatg aacgggttgg cgctcgactc gaaccccaac atgacgcagg ccgggcggcc
     3421 cgcgacctgg ggaacgatgt gcgctccgtt ctcggcggca ccgtcagcca gctcgatgag
     3481 ctgtcgccag ttgtccggct ggatcgggct ctgcaggcag gcgaaggtga cggggcggcc
     3541 ggactcccgt gacacctcgg tcatccaggc gatctccgac accgggtcga acatgtcgga
     3601 ggcgaccgag aacacaccgg cgtcggcctc acccagcgcc cgaccgatgc cgatgagttc
     3661 ggcggcatcc gcgttggtgc ccgctgcgag ctcgccgtcc ttggcgcgat gcagcagggt
     3721 ccgcgtcgtc gagaacccga tggcccccgc ctcgatggcc tcccgcacga tcgtggtcat
     3781 cgcgtcgatc tgctcgggtg tggcctggtc gtgaactgcg gcctggttgc ccatgacgta
     3841 cgcccgcacc gcgccgtggg gcacgagggc cgcgacgtcg aggacccggc ccatgctgtc
     3901 gacctcgtcg aggtactccg ggaaggtctc ccagttccag ctcatacctt cgtcgagcgc
     3961 cgcgcccggg atgtcctcga caccctccat cagctggatg agccactcgc gacgcgccgg
     4021 gtcggcggga gcgaacccga cgccgcagtt gcccatgacg atcgtggtga cgccgtgcca
     4081 ccccgagggg ctgacctcgg ggtcccacgt gacctgcccg tcgtagtgcg tgtggatgtc
     4141 gacccatccc ggcgtcacga ggtttccggc agcgtcgatc tcccgacggc cctccccctc
     4201 gacgacaccg acggcgacga tgcgatcgcc gtccacggcg atgtcaccgg tgaaggccgg
     4261 cgcccccgaa ccgtcgacga tgttggcgtt ccggatcacg agatcgtgca tgcgaggtcc
     4321 cccctcgatc tgagcgtgga accagtctcg tgtcacttga ccgnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnngcaagcg tcgggaacat gaagcaggcc tccatgttct
     4501 gggtgtccat gatcttcacg cgggccgcgg ggtcgcggta gccggggttg atcggctcca
     4561 gctcgccgaa cgcggatcgg atgtcgtcgc taccgctctt gccccggaag tagtcgtcga
     4621 gcacgcccgg cttggcgacg ggatcgaaca gcgggttcgg gatgaaccgg ttgaggcggc
     4681 cgtcgaccat caggcgcgtc ttcccgccga cggtcaccca ctgcatgccg cgcttcgcga
     4741 acttcggttc gatgtgacgg gtgaaggcgt ccggcgcctc gtagtagtgg ttgtcggcgt
     4801 cgaacagctg gaagtcgatc gtggtcatcc ttggccccct gtgagatagg cgccgggtag
     4861 cgctcaccga aaatggtatc tcgttctagt ttgtcgggtc gaagccgctc taggttgtcg
     4921 gcatgatcac cgaccacttc ctcgtgaccg accaggtcgc catcgtcacc ggggccggac
     4981 gcggcatcgg gcgcgccact gcgctggccc tcgcggaggc gggtgccgac ctcgtcatca
     5041 gcgcccgtac ggccgaccag ctcgaggagg tcaagcaaca ggtggcgcgg ctcggccgcc
     5101 gggccgtgtg cgtgaccgcg gacctcagcg acctggcgac cctgccgggc ctcgtcgaga
     5161 cggcgaccgg ccaattcgga cgtctggaca tcgtcgtcaa caacgtcggc ggctccttcc
     5221 ctcgaccgct gctcgagaca tcggcggcca tgttcgagaa ggccttccat ttcaacgtca
     5281 cgacagcgct cgagctgtcg aaggcggcga tcccggcgat gctcgcgagc gatcccgccg
     5341 gtggctcgat cgtcaacatc agctcggcga tcggtcggat gaccgaccgc ggattcgccg
     5401 cgtacggcac cgccaaggcg gcactcaccc atctcactcg actgatggca accgagtgcg
     5461 caccgcgcat ccgcgtcaac gcgatcgccg tcggctcgac ggcgacgagc gcgctcgaca
     5521 tggtcctcac cgacgacggg ctccgcaccg cgatggagga caacacgccc ctccgccgtc
     5581 tcggcgatcc gctcgacatc gccctcgggg tgctctacct cagctccccc gccggctcgt
     5641 acctcacggg caagatcctc gagatcgacg gcggcctcaa cgcgccgaac ctcctgttgg
     5701 gcctgccgga cctgtaggac ggcgctcagt tcaggcagaa ctcggcgacg attcgtgacc
     5761 cgtcgaagtg gacccgcacg gggaacccgc ctccgccgtc gtcggactcg ttccacccgg
     5821 tgatgccgcc gtcgtaggtt ccgtcaggtg ccgttctcag atcctcgggc cgatcgcggt
     5881 gggtcgcgcc gggcgaatcg gtgacctggt cgacccactg ggccagggcg tcgtacgggt
     5941 cgtcgatcga cgagtccgga accagcagat actccccgca cggttcaccg tcgacgggcg
     6001 ctgtgatggc gaacccgtcg ggcggctcga ccggtgcggc ccactcagcg cccgaacagg
     6061 ccgaggtccc gccggccacg gcggtgacca cgaccgcgac accgagcgcg acgcgcttca
     6121 cccgccgacg gtcgcgatcg tggtcgacga ctgggtggcg gcctgcgact gcgcgttgat
     6181 gaggccctcg atccgagact gctccgcctt cgccgtcgtc tggcgggcga gcaggtcctt
     6241 cgccgccacc ggcgtcacca cgtcgatccg gttgcccttc aggcatccga ccacgtcgtc
     6301 ctcggtctca cccacgtcga tgcggtccct gccgacggcg agatcgacga act
//
LOCUS       DAOSOA010000184         6332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12400_length_6332_cov_6.586905, whole genome shotgun sequence.
ACCESSION   DAOSOA010000184 DAOSOA010000000
VERSION     DAOSOA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12400_length_6332_cov_6.586905"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKY13_11300"
     CDS             <1..421
                     /locus_tag="PKY13_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80567.1"
                     /translation="ELVEERPRRGLTERVVRATARSYLVAPDAIDEHAADPSRVDRLS
                     SRYLLAVAVRMVKEVLELSRRAERAEKTLPTLAIDTEIRFASAAERAEFTAELAAAVR
                     DLAARYHDEAAPRGRWHRLVVASHPHPHPPTAQQGAT"
     gene            418..1194
                     /locus_tag="PKY13_11305"
     CDS             418..1194
                     /locus_tag="PKY13_11305"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOT80568.1"
                     /translation="MSESQRSIDLEVVVDGTPEEVWQAIATGPGISSWYVPHVVEERE
                     GGAALASFGEAPAMQIPGRVAAWDPPRRIVFDGGEDAGGLAFEWLVEARDGGTCVVRL
                     VNSGFGDGNEWDDQYDAMTEGWKLFLFNLELHCRHFAGQSATAMLPMGGTPLPQADAW
                     TKLCADLGIPEAPAVGDRVEASAGDGLCLAGTVVRAKPNAIALILDAPAPGTAFLAVE
                     SGGDGCGASIWSYLYGPDAGAIVDRDRPRWQAWLDDFAGT"
     gene            complement(1264..2877)
                     /locus_tag="PKY13_11310"
     CDS             complement(1264..2877)
                     /locus_tag="PKY13_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80569.1"
                     /translation="MKITASPITIRRATLRAVAALTVLGVGSGAFAANAGAAVPERYP
                     LGSIHTLGRTSIDTARITGRAADPDRQTLAVLVQFTVDGRHVATVSANEGGDGHVFDA
                     SLRIDAGAHRVCASALGRGPKQLKAELGCANVGAYAPPAPAPAPAPAPAAAPAPAPAP
                     APAPKPTPKPAPGTPAGGTAERVPMGSIHTAGRTSISTARIVGRAADPDRPTVSVLVK
                     FAVDGRHLATVSANRGADAHGYEANLTIDAKAHQICAVAVGAGPKKLTRELGCANVPA
                     YTPPTTAPAPKPTLPCQSNVILFMPTSMRMLAPPADTCFKWARTDGPGATKPANWPWG
                     ASLRLGFDFSANWGVDRPLPHETKGRSWTFEDMIVSAPGPQPMFKEAAKWINGSPNSS
                     SLGFLQLAVRGNVTRTIESLGVTAPFIPVVQVYRDARGGISDPTSLGYARSLAGKGVL
                     PVFNVSPKNLNASTIATIRKECINFKGRIGVWTGTGSYTISDPAKTNAEIEAFKPMVW
                     KLYQAIDACNQVVGRKSAPTASLAKAFGA"
     gene            complement(3172..3576)
                     /locus_tag="PKY13_11315"
     CDS             complement(3172..3576)
                     /locus_tag="PKY13_11315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT80570.1"
                     /translation="MEIIKLQPAGLVVSPAFSHVAIVPPGATTIYVGGQNGVDETGAV
                     VSDDVGAQASRAIDNAEVALRAAGASLGDVVQWSVVIQQDADLGAAYGAIAPRLATGG
                     APPLVSASKVAALGVPGALLEVSAIAAVLPPE"
     gene            complement(3645..6146)
                     /locus_tag="PKY13_11320"
     CDS             complement(3645..6146)
                     /locus_tag="PKY13_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leishmanolysin-related zinc
                     metalloendopeptidase"
                     /protein_id="HOT80571.1"
                     /translation="MTRRRQVMVVALLATVLASGCSLKEVQTWFALRGHSITAQQAKD
                     IADKVNAQSSAGCDANYADFCVPNNQTSVRCAGTDGAGPEIGPVRIVGWDHFGLDPDG
                     DKRACESPPSATPTPVVSAPTAPPTAPPPLPTTPAPTTTAPSGPIPGTGTSFMYVAAS
                     DADVIRKIDTVTLDVVATYDVGGPLGDGLAMIGTRLFYGAGDRQFMTIGMYDIATGEN
                     NRSYIGGVYEPLLRTSPALPGRLVVATTHTSPSTLYLYDVTGDSGVRLRQSSPHGAIG
                     GNLKEVEISADGTRIWTAAGAPYVISEVRTSDLTLSGGSFETGAYPTATASAIIGGTE
                     LVAAGTAYNRTAFVFNAASRELLTNTPIPGETADRGLQFSRDGSKLYAAVEAGASADL
                     VTIDRATSRVATAPMFGRTLSDLYFDMGELAVDPGTGRVFVTAHDAISVYNPDGTYGR
                     TLSQRGPRSMAFSATGPTSTPPTLVPTSLPNAGPPVISRFDASHSSGPAPLTTAWQWT
                     ISDPDGSPLICSLDLDGNGVVESTISPCTSAAMRTITYTTAGERTARLYVSDGELTTS
                     DGTLVRVFEPSADPFDITIRPNGSMTSSQLAAFTAAAQRWARVIRTGLPDGTVAVEAD
                     ECDDGAPAYTGPVDDLVIEAIITPIDGPDGILGAAGPCLVRSSGGLPAYGIMQFDSAD
                     VAMLEADGSFTTVVLHEMAHVLGFGSVAGWRAILDGAGTANPTFDGAVARAAWSMIGG
                     GSASVPVEGLGGPGTAGGHWRESVLDNELMTGWLNEGPNPLSTVTVGAMADLGYGVDL
                     AGADSYGSAVSAFRALFTPSRKIDVELLSPLDRTI"
BASE COUNT          921 a         2171 c         2284 g          956 t
ORIGIN      
        1 cgaactcgtc gaggagcggc cccgccgggg cctgaccgag cgcgtcgtca gggcgaccgc
       61 gcgctcctac ctcgtcgccc ccgacgccat cgacgagcac gccgccgacc cgtcccgggt
      121 cgaccgcctg tcgagccgct atctcctcgc ggtcgccgtt cggatggtga aggaggtgct
      181 cgagctgtcc cgtcgggccg aacgcgccga gaagaccctg ccgacactcg ccatcgacac
      241 cgagatccgg ttcgcgtcgg cggccgaacg cgctgagttc accgccgagc tcgccgcagc
      301 ggtgcgcgac ctcgccgccc gataccacga cgaggccgcc ccacgagggc gatggcatcg
      361 cctggtcgtc gcgtcccatc cccatcccca cccgccaacc gcgcagcaag gagcaacatg
      421 agcgagtccc aacgatccat cgacctcgag gtcgtcgtcg acggcacccc cgaggaggtg
      481 tggcaggcca tcgcaacggg ccccggcatc tcgtcgtggt acgtccctca cgtcgtggag
      541 gaacgcgaag gcggcgccgc gctcgcatcc ttcggcgaag cgccggcgat gcagatcccg
      601 ggccgggtcg cagcgtggga tccgccccga cgcatcgtgt tcgacggcgg cgaggacgcc
      661 ggcggcctcg cgttcgaatg gctcgtcgag gcgagggacg gcggcacgtg tgtcgtccgg
      721 ctcgtcaaca gcgggttcgg cgacggcaac gagtgggacg accagtacga cgccatgacc
      781 gagggctgga agctgttcct gttcaacctc gagctccact gccgtcactt cgccggacag
      841 tcggcgacgg cgatgctgcc gatgggtggc accccgctgc cccaggccga cgcgtggacc
      901 aagctgtgcg ccgacctcgg cattcccgaa gcccccgccg tcggcgaccg ggtcgaggcc
      961 tccgccggtg acggactgtg cctggccggc accgtcgtca gggcgaaacc gaacgccatt
     1021 gccctgatcc tcgacgcacc agcgccgggc accgccttcc tcgccgtgga atccggcggc
     1081 gacggctgcg gggcgtccat ctggtcctac ctgtacgggc ccgacgccgg cgccatcgtg
     1141 gatcgcgacc ggccccgctg gcaggcgtgg ctcgacgact tcgccggcac ctgacggcat
     1201 cgtcgcgcgg cggaggccgg cccagtgggc cggcctccgc cgcgtcgggt acctccggtc
     1261 ggttcaggcg ccgaacgcct tggcgaggct cgccgtcggg gcactcttcc gaccgacgac
     1321 ctggttgcag gcgtcgatcg cctggtacag cttccagacc atcggcttga acgcctcgat
     1381 ctcggcgttg gtcttggcag ggtcactgat cgtgtacgag cccgtaccgg tccatacgcc
     1441 gatgcggccc ttgaagttga tgcactcctt gcggatggtg gcgatggtcg aagcgttcag
     1501 gttcttcggg gagacgttga agaccgggag cactcccttg ccggcgagcg agcgggcata
     1561 cccgagcgac gtcggatcgg agatcccgcc gcgggcatcc cggtacacct ggaccaccgg
     1621 gatgaagggc gccgtgacac ccagcgactc gatggtgcgg gtcacgttgc cgcgaacggc
     1681 gagttgcagg aagccgagcg aggacgagtt cggcgagccg ttgatccact tggcagcctc
     1741 cttgaacatc ggctgcgggc cgggtgcgga cacgatcatg tcctcgaagg tccaggaccg
     1801 gcccttggtc tcgtgcggca gcgggcgatc gacgccccag ttggcggaga agtcgaagcc
     1861 cagtcggagc gacgctcccc acggccagtt cgccggcttc gtcgcgccgg gtccgtcggt
     1921 gcgggcccac ttgaagcagg tgtcggcagg cggcgcgagc atccgcatcg aggtcggcat
     1981 gaagaggatg acgttcgact ggcaggggag cgtcggcttc ggtgccggag cagtggtcgg
     2041 cggggtgtag gcgggcacgt tggcgcaccc gagctcgcgg gtcagcttct tcggcccggc
     2101 cccgacggcg accgcacaga tctgatgggc cttcgcatcg atggtgaggt tggcctcgta
     2161 cccgtgcgca tcggcaccac gattggccga cacggttgcc agatgccgac cgtcgacggc
     2221 gaacttcacg agcaccgaga ccgtcggtcg atccggatcg gcagcgcgac cgacgatccg
     2281 ggcggtgctg atcgacgtgc ggccggcggt gtggatggag cccatcggca cccgctcggc
     2341 cgtcccgccg gcgggtgtgc ccggcgctgg cttgggggtc ggcttgggcg ctggggcggg
     2401 tgccggtgct ggggccggtg ccgctgccgg ggcgggagcc ggagccggtg ccggtgccgg
     2461 aggcgcgtag gccccgacgt tggcgcatcc caactcggcc ttcagctgct tgggcccacg
     2521 gccgagtgcg ctcgcacaca cccgatgggc gccggcgtcg atgcggaggc tcgcgtcgaa
     2581 cacgtgcccg tcgccgccct cgttggccga gacggtggcg acgtggcgac cgtcgaccgt
     2641 gaactggacc agcaccgcga gcgtctgtcg atcgggatcg gcggcccggc cggtgatgcg
     2701 ggcggtgtcg atcgacgtac ggcccagggt gtggatcgag ccgagggggt agcgctcggg
     2761 gacggcagca ccggcgttcg ctgcgaacgc cccggagccg acaccgagaa cggtgagggc
     2821 cgccacagcc cggagggtgg ctcggcggat cgtgatgggt gaggcggtga tcttcatggt
     2881 gcgctccttg gcgggatcgg gatgcactga gcatccggtc caccgcttca ggcccgattc
     2941 agatcagctt caaccgcccg cctccgaacc gtgccctggc tcaggtacac cgcctgcgcg
     3001 atcgccggta cccactgccc gctgacgagg cgctcggaac tgggtcgacg actcgggtgc
     3061 gcgttccaga gctgttcggt cccgaggtgg cgcgcgacag gggcacgtca ctcgaccgga
     3121 tggttgcctg tcttggcccg cgcccggcgt ggaccggcaa ccacgacgcc gtcactccgg
     3181 cgggaggacc gccgcgatcg cactgacctc gaggagcgcc ccgggcacgc cgagcgccgc
     3241 caccttggac gctgacacga gtggcggcgc accgccggtg gccaaccgcg gcgcgatggc
     3301 gccgtaggcc gcacccagat cggcatcctg ttggatcacg acgctccact ggacgacgtc
     3361 gcccagcgac gcaccggcgg cacggagcgc gacctcggcg ttgtcgatgg cccgactcgc
     3421 ctgcgccccg acgtcgtcgg agacgacggc gccggtctcg tcgacgccgt tctggcctcc
     3481 gacgtagatg gtggtcgccc cgggcggcac gatggcgacg tggctgaacg ccggactcac
     3541 gacgaggccg gcaggttgca gtttgatgat ctccatgccg gccattgtgg caacggccgc
     3601 tgacagtgac gtgggaccgg ccgagaccgc cggccgttga ttgctcagat cgtccgatcg
     3661 agcggcgaca ggagctccac gtcgatcttc cgcgacgggg tgaacagtgc gcggaacgcg
     3721 ctcacggcgc tgccgtacga gtccgcacca gcgaggtcca ccccgtaccc gaggtcggcc
     3781 atcgctccga ccgtgacggt gctcaacggg ttcggtccct cgttcagcca gccggtcatc
     3841 agctcgttgt cgagcacact ctcacgccag tgcccgccgg cggtgcccgg acctcccagc
     3901 ccctccaccg gcaccgaggc ggaaccgccg ccgatcatgc tccacgccgc cctcgccacc
     3961 gcgccgtcga acgtcggatt cgctgtaccg gccccgtcga ggatcgcccg ccagccggca
     4021 acgctcccga accccagcac gtgcgccatc tcgtgcagga ccacggtcgt gaaggaaccg
     4081 tcggcttcga gcatggcgac atcggccgag tcgaactgca tgatgccgta ggcggggagc
     4141 ccgcccgagc tgcgtacgag gcacggtccg gccgcgccga ggatgccgtc ggggccgtcg
     4201 atcggggtga tgatcgcctc gatcacgagg tcgtcgaccg gcccggtgta ggcgggagcg
     4261 ccgtcgtcgc actcgtccgc ctcgacggcg accgtgccgt cgggaagccc cgtccggatc
     4321 acgcgggccc agcgctgggc ggcggctgtg aacgccgcga gctgcgacga cgtcatggac
     4381 ccgttgggtc gaatcgtgat gtcgaacggg tccgcagacg gctcgaagac acggacgagc
     4441 gtgccgtccg acgtcgtgag ttcaccgtcg gacacataca gccgagcggt gcgttccccc
     4501 gccgtggtgt acgtgatggt gcgcatcgcc gcgctggtgc acggactgat cgtcgactcg
     4561 acgaccccgt tgccgtcgag gtccaggctg cagatcaggg gactcccgtc ggggtcggag
     4621 atcgtccact gccacgcggt cgtcagcgga gccggcccac tcgagtgcga cgcgtcgaat
     4681 ctcgagatca ccggcggccc ggcgttgggc agcgaggtcg gaacgagcgt gggaggtgtg
     4741 gaggtcggcc ccgtggcgga gaaggccatc gagcgcggcc cccgctgact gagcgttcgc
     4801 ccgtaggtgc cgtcggggtt gtacaccgag atcgcgtcgt gcgccgtgac gaacacccgg
     4861 ccggtgccgg ggtcgacggc gagctcaccc atgtcgaagt acaggtcgga cagggtcctc
     4921 ccgaacatcg gggccgttgc gacccgtgag gtcgcacggt cgatcgtgac gaggtcggca
     4981 ctcgcccccg cctcgacggc ggcgtacagc ttcgaaccgt cgcgggagaa ctggagccca
     5041 cggtccgcgg tctcgccggg gatcggggtg ttcgtgagga gctcccggct ggcagcgttg
     5101 aacacgaacg ccgtccggtt gtacgccgtc ccggcggcga ccagctcggt gccgccgatg
     5161 atcgccgacg ccgtcgccgt cgggtaggcc ccggtctcga agctcccgcc cgacagcgtc
     5221 aggtcggacg tgcgcacctc ggagatcacg tacggtgcgc ccgccgctgt ccagatccgc
     5281 gtgccgtccg cggagatctc gacctccttc aggttgccgc cgatcgctcc gtgtgggctc
     5341 gactgacgga ggcgaacgcc cgagtcgccg gtgacgtcgt agaggtacaa cgtcgacggg
     5401 ctggtgtggg tcgtggcgac gaccagacga ccggggagcg ccggcgacgt gcgcaggagt
     5461 ggctcgtaga ccccgccgat gtacgaccgg ttgttctcgc cggtggcgat gtcgtacatg
     5521 ccgatcgtca tgaactgtcg gtcgccggcg ccgtagaaga ggcgggttcc gatcatcgcg
     5581 agaccgtcgc cgaggggacc gcccacgtcg taggtcgcga cgacgtcgag tgtgacggtg
     5641 tcgatcttgc ggatgacgtc tgcgtcgctc gctgcgacgt acatgaacga ggtgccggtg
     5701 ccggggatcg ggcccgacgg ggccgtggtc gtcggcgccg gagtcgtcgg gagcggtggg
     5761 ggagcggtcg ggggcgccgt cggtgcgctg acgaccggcg tcggtgtcgc tgagggcgga
     5821 gattcgcagg cccgcttgtc gccgtcggga tcgaggccga agtggtccca gcccacgatg
     5881 cggaccggtc cgatctcggg cccggcgccg tcggtgccgg cgcagcgcac cgacgtctgg
     5941 ttgttgggga cgcagaagtc ggcgtagttg gcatcgcagc cggcggagct ctgggcgttc
     6001 accttgtcgg cgatgtcctt cgcctgctgg gccgtgatcg agtggccccg caacgcaaac
     6061 caggtctgca cctccttgag ggaacagccc gacgcgagca cggtcgccag gagggccacc
     6121 accatgacct gccgtcgtcg tgtcactgca gcaccgctcc ctcgacatct cgtcccgggt
     6181 gcgggaccgg tggaccgtac aacggggttc tccggatgcg aaggaagaac ttcgggtcac
     6241 ccctgagttg ggtgaatctg cccgattcgg ctcgtctgga tctcactgtc aggaccctct
     6301 gcgatactga acccatgacg aacaccagtt cg
//
LOCUS       DAOSOA010000185         6328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12412_length_6328_cov_6.582975, whole genome shotgun sequence.
ACCESSION   DAOSOA010000185 DAOSOA010000000
VERSION     DAOSOA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12412_length_6328_cov_6.582975"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1060
                     /locus_tag="PKY13_11325"
     CDS             5..1060
                     /locus_tag="PKY13_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT80572.1"
                     /translation="MTEHERSTSPTTIDPGAVSTTAVPSTTVHVPGNHLMTQLLGSRD
                     EWLDRVEAAFVGTRIVARGNEINVEGPDAIAVGRIFEELVIFVEGGTKLDEDLLRRTI
                     DMVAVDELPSQLLGSDVVRLARGGSVRPKTAGQRRYIDAIAENIITFGIGPAGTGKSW
                     LAVAMATQALQRKQVERIVLTRPAVEAGERLGFLPGDLMAKVDPYLRPLYDAFYDMVG
                     AEGAAKLLERGTVEVAPLAFMRGRTLNRSFIILDEAQNTTPEQMKMFLTRIGYGSKAV
                     ITGDTTQVDVAGGRSGLDNLEGILQGIDGLSFVRLGSRDVVRHKIVADIVNAYERSSA
                     GSSGASGPSGASSGSSA"
     gene            1060..1560
                     /gene="ybeY"
                     /locus_tag="PKY13_11330"
     CDS             1060..1560
                     /gene="ybeY"
                     /locus_tag="PKY13_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT80573.1"
                     /translation="MEGASPTISVTVLNETSAEIDADRWAELVRGVLEREGVEAPAET
                     AVVFVDDVVMAELNAEHMGGSGPTDVLSFPIDDGADDDLAAIPGQARMVGDIVVCPTV
                     AATNAPDHAGDLDDEIALLLIHGSLHLLGHDHAEVDERTEMWAAERRLVAELWGTFPR
                     DPWQET"
     gene            1557..>2506
                     /locus_tag="PKY13_11335"
     CDS             1557..>2506
                     /locus_tag="PKY13_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT80574.1"
                     /translation="MIAVAFTTADAILLVVVVVLIFLSAAIAVAETAITRISRSRAAA
                     LEEQGHKRAAMVVAIVGNFERSLNAIYLVALALQTVQASITGIVASRVFGTAGVIVAT
                     LINVVVVFIIAEAAPKTWALQHTDRAALATAPLIVLIGRMFRWIADVLITVTNVILPG
                     KGLEQGPFVTEEEIIALMGEAAEASVIEEEERDLIESIIDFGDTVAREIMVPRTDMVS
                     FEVEYRVADCVEIAILNGLSRFPVYRENIDDIVGIVYAKDLMRAERDQRGAETIEGLM
                     RPPQLIPETKRVAELLREMQSRQTHIAIVVDEYGGTAGLVT"
     assembly_gap    2507..2606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2696..3787
                     /gene="era"
                     /locus_tag="PKY13_11340"
     CDS             2696..3787
                     /gene="era"
                     /locus_tag="PKY13_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT80575.1"
                     /translation="MSPDDLDPTPTGGPPMFDDDLVDEFDDIDEEEDEANGAVRYLSV
                     EEFLAGSSEPEVPEGFRSGFVSLVGRPNVGKSTLLNRILGHKVSIVSDKPQTTRHQIR
                     GVLNRPATQIVFVDTPGIHKPRTLMGERLNEAAIGAAKGVDVVCLLVDATAPVGRGDA
                     WVAERVPDDAIVIVNKVDLVSKEQVMKQLLRTTDFGQFGESNGGDASSGPTGLDLVRE
                     VGAGAREYFPVSAATGEGVEALVEHLISRLPEGPKWYPDDMVTDVEDAFWVAELVREK
                     LLAVTHDELPHSIACRVTEWEWPRVRVEILVERESQKGIVIGKKGAVLKQAGIATRAE
                     MPPGAFLELHVKVDKDWQRRPKALDRLGY"
     gene            complement(3794..4210)
                     /locus_tag="PKY13_11345"
     CDS             complement(3794..4210)
                     /locus_tag="PKY13_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOT80576.1"
                     /translation="MNRGPVASGSRGLLRAGWFAGGWVAVGVGGVGIVVPGLPTTVFF
                     IVAAYCFGKSSPRFEQWVLDLPTIGPMVRDHRAGLGMPKRIKVIAISTMWAAIAVSAV
                     VLRERWAIALAVIALGLVGTWYILTRVPTKPDADLA"
     gene            complement(4218..4730)
                     /locus_tag="PKY13_11350"
     CDS             complement(4218..4730)
                     /locus_tag="PKY13_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80577.1"
                     /translation="MSSELIEQTAAAPGPAPAAAGCPVPHGLTKLAPEGVCPFPHDAG
                     AARPAPQRSKADEVVRKVLRIKERPAGVTENAALAAFQKSMLISATRCTLTYVVFPFV
                     LPAVSFAKGVGPILGIIIGVIAMVCDVFTIRRFFAVDHKWRWYFSAAALSVITLLTVL
                     LVQDIVHLVQ"
     gene            complement(4783..>6328)
                     /locus_tag="PKY13_11355"
     CDS             complement(4783..>6328)
                     /locus_tag="PKY13_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT80578.1"
                     /translation="RPRSEKIVLMTDAPWQGDASSLVEAFRKGERSPREELEATLAAI
                     DASDLNAFSHVDPERALAAADHADVNLPFGGVPAGVKELDAVAGWPDTDASLVFKDRI
                     ATTTSPNVERLRDVGGAVLVGLTTASEFGGLNVSVTKLNGVTHNPWRHGRTVGGSSAG
                     SSSAVAGGLVTIATGSDGGGSIRIPAGYTGLFGMKGTFGRIPRGPSAFSRPNTAVQGC
                     LSRSVRDAARFFDVCAGPHPIDPLSITSPGGWEAGLGNHDLAGLKVAVVPGLGGVTLE
                     DGVEQRIREHAAALIEATGMKEVDLDFAVPNITAEWMMGNTSTLLAELGDLWPACAGD
                     LTQSVEDGLRISAAFYNLRTAGVAEHKRLLLNRAMADVFDQVDLVIGATNPGPAFPAE
                     AEMSKPLPPLADKAMSSRPARLGMRGALGAIRVAGSAAPNLPAAVLAKVTAKVPEMLE
                     MGGLTIMSNIYGNPAVSIPAGLVDGLPVGMQVLARHHADDLLFDVGLAAERELPWPIT
                     AAAGRS"
BASE COUNT          992 a         2101 c         2208 g          927 t
ORIGIN      
        1 taacatgact gaacacgagc gatccacatc gcccaccacc atcgaccccg gcgcagtctc
       61 gaccacggcc gtcccgagca ccacggtgca cgtcccgggc aaccatctga tgacgcagct
      121 cctcggctcg cgcgacgagt ggctcgaccg cgtcgaggcc gccttcgtcg gcacacgcat
      181 cgtggcccgc ggcaacgaga tcaacgtcga ggggcccgac gcgatcgccg tcggccgcat
      241 cttcgaggag ctggtgatct tcgtcgaggg cggcacgaag ctcgatgagg acctcctccg
      301 ccgcacgatc gacatggtcg ccgtcgacga gctgccgtcc cagctgttgg gcagcgacgt
      361 cgtccgcctg gcccgtggag gttcggtccg tcccaagacc gccggacagc gccgctacat
      421 cgacgccatc gccgagaaca tcatcacgtt cggcatcggc cccgccggta ccggcaagag
      481 ctggctcgct gtcgccatgg cgacccaggc cctgcaacgc aagcaggtcg agcgcatcgt
      541 cctcacccgc ccggcggtcg aggccggcga gcgcctcggc ttcctgcccg gcgacctgat
      601 ggccaaggtc gatccctacc tccggccgtt gtacgacgcg ttctacgaca tggtcggcgc
      661 cgaaggtgcg gcgaagttgc tcgagcgggg caccgtcgag gtcgctccgt tggcgttcat
      721 gcgtggtcga acgctcaacc gcagcttcat catcctcgac gaggcgcaga acacgacgcc
      781 cgagcagatg aagatgttcc tcacccgcat cgggtacggc tccaaggccg tcatcaccgg
      841 tgacaccacg caggtcgacg tcgccggcgg ccgcagtggt ctcgacaacc tcgaaggcat
      901 cctgcagggc atcgacgggt tgtcgttcgt tcggctcggc agtcgcgacg tcgtccgcca
      961 caagatcgtg gccgatatcg tgaacgcgta cgagcggtca tcggcgggga gttcgggagc
     1021 gtcgggcccg tcaggcgctt cgtcggggag cagcgcctga tggagggagc gtctccgacg
     1081 atctcggtga ccgttctcaa cgagacgtcc gccgagatcg atgccgatcg ctgggccgaa
     1141 ctcgtccgcg gtgtgctcga acgcgagggg gtcgaagccc ccgccgagac cgccgtggtc
     1201 ttcgtcgacg acgtcgtgat ggccgagctc aacgccgagc acatgggcgg gtccggcccg
     1261 accgacgtgc tgtcgttccc gatcgacgac ggtgccgacg acgacctggc cgccatcccc
     1321 ggccaggccc gaatggtcgg tgacatcgtg gtgtgcccga cggtcgccgc caccaacgcg
     1381 cccgaccacg ccggcgacct cgacgacgag atcgcactcc tcctcatcca cggttcgctc
     1441 cacctcctcg gtcacgacca tgccgaggtc gacgagcgga cggagatgtg ggcggccgag
     1501 cgccgcctcg tcgccgagtt gtggggcacc ttccctcgag atccctggca ggagacatga
     1561 tcgccgtcgc cttcaccacc gccgacgcca tactgctcgt cgttgtggtg gtgctcatct
     1621 tcctgagcgc cgcgatcgcc gtcgccgaga ccgccatcac ccgcatcagc cggtcgcggg
     1681 ccgcggcgct cgaggaacag gggcacaagc gcgccgccat ggtcgtcgcg atcgtcggca
     1741 acttcgagcg gagcctcaac gccatctatc tggtcgcgct ggcgctgcag acggtgcagg
     1801 cgtccatcac cggcatcgtc gcgtcgcggg tgttcggcac cgccggcgtc atcgttgcga
     1861 cactgatcaa cgtcgtggtg gtgttcatca tcgccgaggc cgcgccgaag acgtgggccc
     1921 tgcagcacac cgaccgcgcc gcgctggcca cggcgcccct catcgtgctc atcggccgca
     1981 tgttccggtg gatcgccgat gtgctcatca ccgtcaccaa cgtgatcctg ccgggcaagg
     2041 gtctcgagca gggcccgttc gtgacggagg aggagatcat cgccctgatg ggtgaggccg
     2101 ccgaggcctc ggtcatcgag gaggaggaac gcgatctcat cgagtcgatc atcgacttcg
     2161 gcgacacggt ggcccgcgag atcatggtgc cccgcaccga catggtgtcc ttcgaggtcg
     2221 agtaccgcgt cgccgactgc gtcgagatcg ccatcctcaa cggactgagc cgcttcccgg
     2281 tgtaccgcga gaacatcgac gacatcgtcg gcatcgtgta cgccaaggat ctgatgcggg
     2341 cagagcgcga ccaacgagga gcggagacca tcgaaggtct catgcgtcct ccccagctga
     2401 tccccgagac caagcgcgtc gccgagctcc tgcgcgagat gcagtcccgc cagacccaca
     2461 tcgcgatcgt cgtcgacgag tacggcggca ccgccggcct cgtgacnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnntact cgctcgaggt gcagtcggtc gacggtcgcc
     2641 gcgtgggccg ggtcaaggtc gtggcggtca cggtcgacga caaggcgcca gcggaatgag
     2701 tcccgacgac ctcgacccga cgcccaccgg cgggcccccg atgttcgacg acgacctcgt
     2761 cgacgagttc gacgacatcg acgaggagga ggacgaggcc aacggtgccg tccgctacct
     2821 ctcggtcgag gagttcctcg ccggctcgtc ggagcccgag gtgcccgagg ggttccgctc
     2881 gggcttcgtg tccctggtcg gtcgccccaa cgtcggcaag tcgacgctgc tcaaccgcat
     2941 cctcggccac aaggtctcga tcgtctccga caagccgcag accacacgtc accagatccg
     3001 cggcgtcctc aaccggccgg cgacccagat cgtcttcgtc gacacgcccg gcatccacaa
     3061 gccccgcacg ctcatgggag aacgcctgaa cgaggcggcc atcggcgccg cgaagggcgt
     3121 cgatgtcgtg tgtctgctgg tcgatgcgac ggcgcccgtc ggccgcggtg acgcgtgggt
     3181 cgccgagcgg gtccccgacg acgccatcgt catcgtcaac aaggtcgacc tggtctccaa
     3241 ggaacaggtc atgaagcagc tgctgcggac gaccgacttc gggcagttcg gcgagtcgaa
     3301 cggcggcgac gcgtcctcgg gcccgaccgg cctcgatctc gtacgcgagg tcggcgcggg
     3361 ggcccgcgag tacttccccg tgtcggcggc gacgggcgag ggcgtcgagg cgttggtcga
     3421 gcatctgatc tcccgcttgc ccgaaggccc caagtggtac cccgatgaca tggtgaccga
     3481 cgtcgaggat gcgttctggg tggccgagct ggtgcgcgag aagctgctgg cggtgaccca
     3541 cgacgagttg ccgcactcga tcgcctgccg ggtgacggag tgggagtggc ctcgggtccg
     3601 cgtcgagatc ctcgtcgaac gcgagtccca gaagggcatc gtgatcggca agaagggcgc
     3661 cgtgctcaag caggcgggca tcgccacccg ggccgagatg ccgccggggg cgttcctcga
     3721 gctgcacgtc aaggtcgaca aggactggca gcgtcgtccc aaggccctcg atcgtctcgg
     3781 ctactgaacc ggttcaggcg aggtcggcgt cgggtttcgt cgggacccgg gtgaggatgt
     3841 accaggtgcc gacgagtccg agggcgatga ccgcgagtgc gattgcccat cgttcccgca
     3901 ggaccacggc gctcacggcg atcgccgccc acatcgtcga gatggcgatg accttgatgc
     3961 gcttcggcat gccgaggccg gcgcggtggt cgcgaaccat ggggccgatc gtggggaggt
     4021 cgaggaccca ctgctcgaac cggggactcg acttgccgaa gcagtaggcg gccacgatga
     4081 agaagacggt cgtcggcagc ccgggcacga cgatgccgac tccaccgacg cccacggcga
     4141 cccagccgcc ggcgaaccac ccggcgcgga gcagcccgcg gctgccggac gcgaccggcc
     4201 ctcggttcat gccccggtca ctgcaccagg tggacgatgt cctgcacgag caggacggtg
     4261 agcagcgtga tgacgctgag ggcggccgcc gagaagtacc agcgccactt gtgatcgacg
     4321 gcgaagaagc gccggatcgt gaacacgtcg cacaccattg cgatgacgcc gatgatgatg
     4381 ccgaggatcg ggccgacacc cttggcgaag ctcaccgccg gcagcacgaa cggaaacacc
     4441 acatacgtga gggtgcaacg tgtcgccgag atcagcatcg acttctggaa cgcggcgagc
     4501 gccgcgttct cggtgacgcc ggcggggcgc tccttgatgc ggagcacctt gcggacgacc
     4561 tcgtcggcct tcgaccgctg cggagcgggg cgggcggctc cggcgtcgtg ggggaacggg
     4621 cagacaccct cgggcgccag cttcgtgagg ccgtgcggga cggggcaacc ggctgcggcc
     4681 ggcgccggtc cgggtgcggc cgccgtctgt tcgatcagct cggatgacac gggtgcagtc
     4741 tacgaccagg ccgggatcgc cagcgattcg ggtgcggaac ggtcaggacc gtccggcggc
     4801 ggccgtgatc ggccacggca gctcgcgctc agcggcgagg ccgacgtcga agaggaggtc
     4861 gtcggcgtga tggcgtgcca gcacctgcat gccgacggga agcccgtcga cgaggcccgc
     4921 cgggatcgac accgcggggt tgccgtagat gttcgacatg atggtgaggc cgcccatctc
     4981 gagcatctcg ggcaccttgg cggtcacctt cgcgagcacg gcggcgggca ggttgggcgc
     5041 cgcactgccg gcgacgcgga tggcgccgag tgcaccgcgc atgccgaggc gggccggccg
     5101 gctcgacatg gccttgtcgg cgagcggggg cagcggcttg ctcatctccg cctcggcggg
     5161 gaaggccggg ccggggttgg tggcgccgat gacgaggtcg acctggtcga acacgtcggc
     5221 catggcgcga ttgagcagga ggcgcttgtg ctcggcgaca ccggccgtgc ggaggttgta
     5281 gaacgcagcg ctgatgcgca ggccgtcctc gaccgactgg gtgaggtcgc cggcgcacgc
     5341 tggccagagg tcgccgagtt cggcgagcag cgtggacgtg ttgcccatca tccactcggc
     5401 cgtgatgttc ggcacggcga agtcgaggtc gacctccttc atgccggtgg cctcgatgag
     5461 ggccgctgcg tgttcgcgga tgcgctgctc gacgccgtcc tcgagggtga ctccgccgag
     5521 cccgggcacc acggccacct tgagaccggc caggtcgtgg ttgcccaggc cggcttccca
     5581 gccgccgggg gaggtgatgc tcagcggatc gatcgggtgg ggaccggcgc agacatcgaa
     5641 gaagcgggcg gcgtcgcgca ccgagcgcga caggcagccc tggaccgccg tgttcgggcg
     5701 ggagaaggcg gacggcccgc gggggatgcg gccgaacgtg cccttcatgc cgaagagccc
     5761 ggtgtagccg gcggggatgc ggatcgagcc accgccgtcg gaaccggtgg cgatcgtgac
     5821 gaggccaccg gcgactgcgg acgagctgcc ggcggaggag ccgccgaccg tgcgaccgtg
     5881 ccgccacggg ttgtgggtca cgccgttgag cttcgtaacg ctcacgttga ggccgccgaa
     5941 ctcactggcc gtcgtcagac cgacgagcac ggcgccgccg acgtcgcgga gccgttcgac
     6001 gttgggcgac gtggtggtgg cgatccggtc cttgaagacg agcgatgcgt cggtgtcggg
     6061 ccacccggcg acggcgtcga gctctttgac gccggcgggt acgccgccga acggcaggtt
     6121 gacgtcggcg tggtcggcgg cggcgagggc acgctcgggg tcgacgtggc tgaacgcgtt
     6181 gaggtcgctg gcatcgatcg ctgcgagggt cgcctcgagc tcctcgcggg gcgagcgttc
     6241 gcccttgcgg aaggcctcga cgagggagga ggcgtcacct tgccacggag cgtcagtcat
     6301 gagaacgatc ttctcactcc gcggccga
//
LOCUS       DAOSOA010000186         6317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12445_length_6317_cov_7.481635, whole genome shotgun sequence.
ACCESSION   DAOSOA010000186 DAOSOA010000000
VERSION     DAOSOA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12445_length_6317_cov_7.481635"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKY13_11360"
     CDS             complement(<1..274)
                     /locus_tag="PKY13_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80579.1"
                     /translation="MFDVTVLTEMTAKIAGLDEHLYADDDLLAGVKAIETAVQSLQAA
                     QGALLGELDARGTTDVVSGHRTANWLARETGIAPAAAKARVRLAVSL"
     gene            complement(355..966)
                     /locus_tag="PKY13_11365"
     CDS             complement(355..966)
                     /locus_tag="PKY13_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT80580.1"
                     /translation="MGFYEDQVLPRVIDKLLGNREMGKLRARALEGVSGTVLEIGFGS
                     GTNLEHYPREVDRVLAVDPATVGRKLARKRLATARVPVEFIGLDGQSLPLDDESVDNA
                     VSTWTLCTIPDVAAALAEVRRVLKPGGRLYFLEHGLSDDPKVARRQHRFDPWQQRVAG
                     GCHLDRRHDDLIRAAGFELDRFSTFAIAGPKSMSFMYAGTAAR"
     gene            1100..1945
                     /locus_tag="PKY13_11370"
     CDS             1100..1945
                     /locus_tag="PKY13_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80581.1"
                     /translation="MKIPIEKFQEQGGRVQTDDLDFDSFRDQPLDDNVLRCIAYMHDI
                     EYQTVLYSRELLMLPQWKDPQFTAFITLWNYEEYWHGQALGRVLEMHGRPAHDERLVA
                     MRTFGKRYLFWSPAIFWLLGHGIRRFPAVHMTWGAINEWTANAAYNRLGQIADHPTLS
                     TLLGRIMRQEGRHAGFYSAWARELLEDDARSRWITKQFLLRQWDIVGNGDVPRSETAF
                     AGAYLFGGDEGNALIDRVEARIDALPGLAGLGLVRTAIKTATDEVTQTEGPGVLARPT
                     GATAA"
     gene            1929..3572
                     /locus_tag="PKY13_11375"
     CDS             1929..3572
                     /locus_tag="PKY13_11375"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT80582.1"
                     /translation="MRPPRDRNVTAPASDPTTESAWAPLRITIFRWLWIASLASNVGT
                     FMHNVGASWLMTDLTRSPTLISLLQTVYAAPGFLLALPAGALADVVDRKKLLVASQSF
                     MLAVATTMGVLTLTDRITTTWLLLLTFLLSVGGTVNMPVWTSITPEVVPRDELPQAVA
                     LNSISSNLAQSVGPAVAGLLIAASGSGAVFLVNAASFVGVVVVVSSWRREAPVTTMPA
                     EHIWSAIRTGLRFTRHSPSLLVLLGRLALFVLFSSSLSALLPLLARTRLQVTAGGFGL
                     LEASLGVGAVAMASVLPKVRKRLGPDGVILFGSIVYGAGLALVAQATSLGPAVLGLLI
                     VGAATISVMTMVFATLQGILPAWVRGRGLAVAMLVVWLITSPAAYGWGALAQAAGVRT
                     ALTVAAAACAAAALVAARPLRIGGFTDVDITPVPPTMPLLACPPAPLDGPVLVTVEWR
                     IDPSDTEAFTEAMAPIRRRRRRDGAMSWGLFRDIERPDRMLESFTVASWAEHERQHGR
                     AIAADVEAEAAAHAFLTHGPPVVTHLVATRRPRAHQLRT"
     gene            3591..3971
                     /locus_tag="PKY13_11380"
     CDS             3591..3971
                     /locus_tag="PKY13_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80583.1"
                     /translation="MSFVHRSRRMGERLAVHGLVLDWNTRGRRSPRWKRRQFDEADVI
                     DVSVSGAQFLAPDNLSIIVGQKVTIVAGGAIGVVRVKRIVPVTNGPLALYGTEYITLE
                     PALEALLFSRLDEVRRPPDNVVWR"
     gene            complement(3994..5130)
                     /locus_tag="PKY13_11385"
     CDS             complement(3994..5130)
                     /locus_tag="PKY13_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOT80584.1"
                     /translation="MRRRFAVPAVIGSVLSLAAVTGCPVEPVTPDPGAPTFTVSTVIS
                     GQVNPWDIAWTPDGTMLFTERPGRINAMVGGAKRVLADPVDAVNTSEGGTLGLAVDPD
                     FATNRRIFVCFESNISGALDERVASYVVDAGFTTLTGRSDIVTGIPLNTSGQAGRHSG
                     CRLAFGPDGYLYVTTGDAVRSDTAQNPTSLGGKVLRVSTTGAGAPGNPGGALDARIYT
                     LGHRNPQGIAFRPGDGAGFSIEQGTGCDDEVNLLAAGANYGWNPGAAGFYNENAPMTD
                     TGSVPGAVAAKWSSGCPTIATSGGGFVTGVQWGSWNGALIAAALAGNQLRVLHFDGAG
                     NLTSQSTALTDRGRLRTIKQGPNGHLYVLTDSSSGSILELTPTP"
     gene            5274..>6317
                     /locus_tag="PKY13_11390"
     CDS             5274..>6317
                     /locus_tag="PKY13_11390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT80585.1"
                     /translation="MTGIPEQRDLLVIGGGPAGAATAIRAARAGARVTLFEKGSPGRD
                     KICGDGLTPRAVKALNELGVDLGDAHRIDGLRMIANNTRRQLNWPGIAPFPAYGAVWP
                     RRRLDEALVRLAEESGAEILWDHEAMPTLEGDRVVGVEVGDRRWTAPFTVVATGAPGP
                     VTRLLGADRDPNEPFGLAIRTYAETPRHAERFLEACLTLRDENGTPVPGYGWMFPAGD
                     GTVNIGVGALSTMKGFKKLNLNKLQDSYRALVQDEWELGPDLERPRAWRLPMSVTRRH
                     GPGWVVVGDAAGLVNPMNGEGIDYGLESGMLAADLFVADPSTAAERYDIEIGERFDSF
                     LRTGRRFSFLIGHP"
BASE COUNT          947 a         2233 c         2140 g          997 t
ORIGIN      
        1 gcaacgacac cgccaaccgc acccgggctt tcgcagccgc tggtgcgatc ccggtctcgc
       61 gggccaacca gttcgcggtg cgatgaccgg agacgacatc ggtggtgcca cgggcgtcga
      121 gttcacccaa caacgcgccc tgcgctgctt ggagggactg gaccgcggtc tcgatcgctt
      181 tcacgccggc caacaggtcg tcatccgcgt acaggtgctc gtcgaggccg gcgatcttcg
      241 ccgtcatctc ggtcagcacc gttacatcga acatgcgttc tattgtacgg gtcaggtctg
      301 acactgaagc cggtggcgaa ctcgatgctc gcgaccggca ctgcggcgcg agaactaccg
      361 ggcggcggtc cccgcgtaca tgaagctcat cgacttcgga ccggcgatgg cgaacgtgga
      421 gaagcggtcg agctcgaacc cggcggcccg gatgaggtcg tcgtggcgac gatcgaggtg
      481 gcacccgccg gcgacgcgtt gttgccacgg atcgaagcgg tgctggcgcc gagcgacctt
      541 gggatcgtcg gacaggccgt gctcgaggaa gtagaggcga ccgcccggct tgagcacccg
      601 acgcacctcg gcgagcgctg ccgcgacatc ggggatcgtg cacagcgtcc aggtgctcac
      661 ggcgttgtcg accgactcgt cgtcgagcgg cagcgactgt ccgtccagcc cgatgaactc
      721 gaccggcacg cgggcagtgg cgaggcgctt gcgggccagc ttgcggccga cggtcgccgg
      781 gtcgacggcg agcacgcgat cgacctcacg gggatagtgc tcgaggttgg tgcccgaacc
      841 gaacccgatc tcgagcaccg tgcccgacac gccctcgagt gcccgggcac ggagcttgcc
      901 catctcgcga ttgccgagca acttgtcgat gacccgcggc aggacctgat cctcgtagaa
      961 ccccatggcc gccagcctcc cacaggaacc ggcgccagga tcgactggat tcaggtcgcc
     1021 gggttcacga aacacccccg gctgacgaaa tcccccgagg ggtagtctcg aattcacggc
     1081 gacacctacg gagcccgcaa tgaagatccc catcgagaag ttccaggagc aggggggtcg
     1141 agtccagacc gacgacctcg acttcgattc gttccgagac cagccactcg acgacaacgt
     1201 cctgcggtgc atcgcgtaca tgcacgacat cgagtaccag acggtgctgt actcccgaga
     1261 gctgttgatg ctgccgcagt ggaaggaccc ccagttcacg gcgttcatca cgctgtggaa
     1321 ctacgaggag tactggcacg gacaggcgct ggggcgcgtg ctcgagatgc acgggaggcc
     1381 ggcgcacgac gagcgactcg tcgccatgcg caccttcggc aagcggtacc tgttctggtc
     1441 gcccgccatc ttctggctgc tcggtcacgg catccggcgg ttccccgccg tgcacatgac
     1501 gtggggcgcc atcaacgaat ggacggcgaa cgccgcgtac aaccggctcg gccagatcgc
     1561 cgaccacccc acgctcagca ccctgctcgg tcggatcatg cggcaggagg gccggcacgc
     1621 gggcttctac tcggcctggg cccgtgagct gctcgaggac gatgcccgct cgcgctggat
     1681 caccaagcag ttcctcctcc gccagtggga catcgtcggc aacggcgatg tcccccgcag
     1741 cgaaacggcg ttcgccggcg cgtatctctt cggcggcgac gagggcaacg cgctcatcga
     1801 ccgggtcgag gcccgcatcg atgcactccc gggcctggcg gggctcggcc tcgtgcgcac
     1861 tgcgatcaag acggcgaccg acgaggtcac gcagaccgaa ggccccgggg tgctcgcccg
     1921 acccaccggt gcgaccgccg cgtgaccgca acgtgacggc tccggcatcg gatccgacca
     1981 ccgaatccgc ctgggcgccg ctgcgcatca ccatcttccg ttggctgtgg atcgcgagcc
     2041 tcgcgtcgaa cgtcggcacg ttcatgcaca acgtcggcgc gtcgtggctg atgaccgacc
     2101 tcacccggtc gcccaccctc atctccctac tgcagacggt gtacgcagcg ccgggcttcc
     2161 tgctcgccct cccggccggc gccctggccg atgtcgtcga ccgcaagaag ctgctcgtcg
     2221 catcgcagtc gttcatgttg gccgtcgcga cgacgatggg cgtcctgacg ctgaccgatc
     2281 gcatcacaac gacgtggttg ctcctgctca cgttcctcct ctcggtcggc ggcacggtca
     2341 acatgccggt gtggacgtcg atcacgcccg aggtcgtgcc ccgcgacgag ctcccccagg
     2401 ccgtcgcgct caattcgatc tcgtcgaacc tcgcgcagtc ggtcggcccc gctgttgccg
     2461 gcctgctgat cgcggcgtcg gggtccggcg cggtgttcct cgtgaacgcg gcgtcgttcg
     2521 tcggcgtcgt ggtggtcgtg tcgagttggc gacgcgaggc tccggtgacc acgatgccgg
     2581 cggagcacat ctggtcggcc atccggacgg gcctccggtt cacccgccac tccccctcgc
     2641 tcctcgtact gctcggacgg cttgcgctgt tcgtgctctt ctccagctcg ctgtccgcgc
     2701 tgttgccgct cctcgcccgc acccgactgc aggtgaccgc cggcgggttc ggcctgctcg
     2761 aggcgtcgct cggggtcggg gccgtggcga tggcgtcggt gttgccgaag gtccgcaagc
     2821 gcctcggacc cgacggcgtc atcctgttcg gctcgatcgt ctacggggcc ggactcgcgc
     2881 tggtcgccca ggccacgagc ctcggtccgg cggtgctcgg cctcctgatc gtgggcgccg
     2941 cgacgatctc ggtcatgacc atggtgttcg ccacactgca gggaatcctc cctgcctggg
     3001 tgcgcggtcg tggcctggcc gtcgcgatgc tcgtcgtgtg gctgatcacg tcccccgcgg
     3061 cctacggctg gggtgcgctc gcgcaggcgg cgggggtccg gacggcactg acggttgccg
     3121 ccgcggcgtg cgccgcagcg gcgctcgtcg ctgcccgccc attgcggatc ggcgggttca
     3181 ccgacgtcga catcactccc gtcccaccca cgatgcccct gctggcctgc ccgcccgccc
     3241 cgctcgacgg tcccgtgctc gtcaccgtcg agtggcggat cgacccgtcc gacaccgagg
     3301 cgttcaccga ggcgatggct ccgatccgcc ggcgccgtcg acgcgacggt gccatgtcgt
     3361 ggggactgtt ccgcgacatc gaacgacccg accggatgct cgagtcgttc accgtcgcgt
     3421 cgtgggcgga gcacgaacgc cagcacggac gagccatcgc cgccgacgtc gaggcggagg
     3481 ccgccgcgca cgcgttcctc acccacggcc ccccggtggt gacccatctc gtcgcgaccc
     3541 gacgacctcg ggcccatcaa ctccggacgt gaccggccga tagatcggcc atgagcttcg
     3601 tgcaccgatc acgccgcatg ggcgagcgac tcgccgtcca cggtctcgtc ctcgactgga
     3661 acacccgggg acgacgcagc ccccgttgga agcgccggca gttcgacgag gccgacgtca
     3721 tcgacgtgtc ggtctccgga gcgcagttcc tcgcccccga caacctgagc atcatcgtgg
     3781 gccagaaggt caccatcgtt gcgggcggcg ccatcggcgt cgtacgggtg aagcggatcg
     3841 tgcccgtgac caacggcccg ttggcgctct acggcaccga gtacatcacc ttggagccgg
     3901 cgctcgaagc actgctcttc tcgcggctcg acgaagtgag gcgcccgccc gacaacgtcg
     3961 tctggcgctg atcgagccgc accggctctc gacctacggg gtcggcgtga gttcgaggat
     4021 cgacccggaa ctcgaatcgg tcaggacgta cagatggccg ttgggaccct gcttgatcgt
     4081 gcgcagccgg ccccggtcgg tgagcgctgt cgactgcgac gtgagattgc ccgccccgtc
     4141 gaagtgcagc acgcggagct gattgccggc gagggcggct gcgatcaacg caccgttcca
     4201 cgatccccac tggacgccgg tcacgaaccc gccgcccgac gtcgcgatcg tggggcaccc
     4261 cgatgaccac ttcgcagcga ccgcaccggg cacactgccc gtgtcggtca tcggcgcgtt
     4321 ctcgttgtag aagccggccg caccggggtt ccatccgtag ttggctccgg cggcgaggag
     4381 gttgacctcg tcgtcgcacc cggttccctg ctcgatcgag aagccggcgc cgtcgcccgg
     4441 tcggaacgcg atgccctgcg gattgcggtg gccgagcgtg tagatgcggg cgtcgagtgc
     4501 tccgccgggg ttgcccggag cgccggcgcc ggtcgtcgag actcgcagca ccttgccgcc
     4561 gagcgaggtg gggttctggg cggtgtcgct ccgcacggca tcgccggtgg tgacgtacag
     4621 atacccgtca gggccgaagg caagccggca gcccgagtgt cggccggcct gtccgctggt
     4681 gttgagcggg atgccggtga cgatgtcgga acgaccggtg agggtggtga agccggcatc
     4741 gacgacgtag gacgcgaccc gctcgtcgag cgcaccgctg atgttcgact cgaagcacac
     4801 gaagatgcga cggttggtcg cgaagtcggg gtcgaccgcc agcccgagcg ttccgccctc
     4861 ggaggtgttg acggcgtcga ccggatcggc gagcactcgt ttggcgccac ccaccatcgc
     4921 gttgatccgg cccggacgtt cggtgaagag catcgtcccg tcaggggtcc aggcgatgtc
     4981 ccacgggttg acctgtccac tgatcaccgt ggacacggtg aacgtggggg cgccggggtc
     5041 gggcgtgacc ggctcgacgg ggcagccggt gacggcggcc agcgagagga cactgccgat
     5101 gacggctgga acagcaaacc tgcgacgcac gggcacaacc ccctgatgga gaccgacccg
     5161 gacgggcgga ccatcactcc taccacctcc ggagtcgcgc cagacgggct tcttcgcgtt
     5221 gggtgccatt ccgatctggc gcgcttcgac cggtggctgc aagggtgatc ggcgtgaccg
     5281 gcatccccga acagcgcgat ctgctcgtca tcggcggcgg tcctgccggc gcggcgacgg
     5341 cgatccgtgc ggcccgggcc ggcgctcgcg tcacactctt cgaaaagggt tcgcccggtc
     5401 gcgacaagat ctgcggcgac ggcctcactc cccgagccgt caaggcgctg aacgagctcg
     5461 gcgtcgatct cggcgacgcc catcgcatcg acggcctgcg catgatcgcc aacaacacac
     5521 gccgccagct caactggccg gggatcgcgc cgttcccggc ctacggcgcc gtgtggccgc
     5581 gtcgtcggct cgacgaggcg ctggtgcgat tggccgagga gtccggcgcc gagatcctgt
     5641 gggaccacga agcgatgccg accctcgagg gcgaccgcgt cgtgggcgtc gaggtgggtg
     5701 atcgccgctg gaccgccccg ttcacggtcg tcgccaccgg tgcgcccggt ccggtgacgc
     5761 gcctgctcgg ggccgatcgc gatccgaacg agccgttcgg cctcgccatc cgcacctacg
     5821 ccgagacgcc tcgccatgcc gaacgcttcc tcgaggcctg cctcacgctc cgcgacgaga
     5881 acggcacccc ggtccccggc tacggctgga tgttccctgc aggcgacggc acagtgaaca
     5941 tcggcgtcgg cgccctgtcg accatgaagg gcttcaagaa gctcaacctc aacaagctgc
     6001 aggacagcta ccgggcgctg gtgcaggacg aatgggagct cggccccgac ctcgaacgtc
     6061 cccgcgcctg gcgcctgccg atgagcgtga cccgccggca cgggccggga tgggtcgtcg
     6121 tcggcgacgc cgccggactc gtcaacccga tgaacggtga gggcatcgac tacggactcg
     6181 agtcgggcat gttggccgcg gacctgttcg tcgccgaccc ctcgaccgca gcagagcggt
     6241 acgacatcga gatcggcgag cgcttcgact cgttcctgcg gaccgggcgc cggttctcgt
     6301 tcctgatcgg ccacccg
//
LOCUS       DAOSOA010000187         6290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12505_length_6290_cov_6.979631, whole genome shotgun sequence.
ACCESSION   DAOSOA010000187 DAOSOA010000000
VERSION     DAOSOA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12505_length_6290_cov_6.979631"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PKY13_11395"
     CDS             complement(<1..1238)
                     /locus_tag="PKY13_11395"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT80586.1"
                     /translation="MSATGEVEHLDVLIVGAGLSGIGAAYHVQNETPWASYALFESRD
                     AIGGTWDLFRYPGIRSDSDMFTLGYPFRPWNRPESIADGGAILQYIKDTASEFGIDRK
                     IRFRHRIVRAEFSSDTALWTVTAERTDGTDTAGGGGETFELTCSFLYSCSGYYRYDQG
                     YLPEFEGMDDFGGTIVHPQKWPEDLDYRDQRIVVIGSGATAVTLIPSLAPTAGHVTML
                     QRSPTYVASLPGKNPLTALVRKIVPARYEGPVLKWMNALATQGSYQLSRRRPELMKRI
                     LRKGVERQLPAGYDVDTHFTPKYDPWDQRLCVVPNGDLFRAIRSGSASVVTDHIDRFT
                     PTGIRLSSGQELEADIIVTATGLDVLFLGGIELFVDGEKVDPSSRLVYKGMMIEDVPN
                     LALAIGYTNASWTLKADLIAG"
     gene            complement(1235..>1633)
                     /locus_tag="PKY13_11400"
     CDS             complement(1235..>1633)
                     /locus_tag="PKY13_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOT80587.1"
                     /translation="QNVFLWHALEESEHKAVAFDVYKAVGGSERLRVWTMKATRLGFV
                     VGMSISVMLSVLGDKAAYRDGNLRRSLKKLRTSPLVTKQVWRDLCDYDRPDFHPDDRD
                     TTALTDEWRDRLFGETGTLVGLLPGGSSAA"
     assembly_gap    1634..1733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1734..1996)
                     /locus_tag="PKY13_11405"
     CDS             complement(<1734..1996)
                     /locus_tag="PKY13_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOT80588.1"
                     /translation="MTITDAPRQTSNPERHVPTRRVSFEESLRNVPKHFAADGDLIES
                     HLAVCMSAVFPDGEDFFVRSVRHYRDRITDPALKRQVAGFIGQ"
     gene            2079..2687
                     /locus_tag="PKY13_11410"
     CDS             2079..2687
                     /locus_tag="PKY13_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT80589.1"
                     /translation="MTTAATSPSSKGELTRQSILDAAIVRFGRDGYRATSVADIARDA
                     AVSGTAAYAHFPNKEALFLAALDDDAAAVISEGVLHLLDDSVASAEWHETLILTLVAA
                     VDRHPLARRVIAGLEPDVTDRVIDIPAMLELRKTVAARLRADQAAGRVRPDIDASAIA
                     DGAVGIIVSLLMAVIQLGTAAVGVYGEGIWAVFTAALDPLPD"
     gene            complement(2692..5061)
                     /locus_tag="PKY13_11415"
     CDS             complement(2692..5061)
                     /locus_tag="PKY13_11415"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT80590.1"
                     /translation="MSVLVLPPTASVPRRARSPMTPVRLGVAVLALAASWSLVTATGG
                     DHSQFRANAGPLLRSAPEWVVDTSVTVAQIGVGLSAFVGLIALLATGRFERVGGVLLG
                     AALSGASMAAIGAVSAEGNFHVARPAVVWFGVGLGFPSLIGIAAIVSAVVVDSPWWSA
                     RMRRSGYALVVAAALARIVSTLAEPATVLVACAVGWVVGHAILAVGGMPNLRPACEDV
                     AAVLSDHGFDAVSIADVSGLAPGYVAMVASDAGGRGVFIKVLTRESWKSRRLARLTHW
                     IRFRDVGEDRPFGSVKHPMDREALGALKAFADGVPTARLVASTNVGTASRLLAYEMFD
                     VRPLTQLDDHEWTPMLLTEIWRSTAALRASRTAHHRLSFDHMLLDGDGHVRIVDFESA
                     ELGAPERMLNADVAEVLAATAARFGASASVRAAIMAVGIDAVTAAAPRLQPLALTPPT
                     RALVKRSGVLPELTAEVTRSTGVPDVPLERLQRVKGSTIFLLATSAVAAWALVPQLIG
                     AGDVWGLVRNANWGWALAALAVSALTYVAAAVAFAGSLPEPIPLGPNVEVQLATSFVA
                     VAATAASLALSARFLHKRGIDAAVSVAAVSINSLAGVVVHLGLLGVFLGLGGTDLLER
                     FTLPSASTILIVVAAIVTTVLVSLAVPTLRRLASLHVVPPVQRALHGLSFVAHSPTNV
                     AELLGGSAGVTLGYVVALAASAHAFGSTLPIITIALVYLLASVVSVAAPTPGGLGAVE
                     ATLIAGLTAAGMKADMALGSVLLFRLATFWIPLLPGWLAYQRLVREGAI"
     gene            complement(5115..>6290)
                     /locus_tag="PKY13_11420"
     CDS             complement(5115..>6290)
                     /locus_tag="PKY13_11420"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOT80591.1"
                     /translation="KEGAVMRTVINDQLISWASDIEPGTLRQAEKTARLPIIAGHVAL
                     MPDAHVGIGATVGSVIPTRGAVIPAAVGVDIGCGMIAAELDVHQDQLPDDLEPLLARI
                     ARVIPAGMGQGHGDVGRRADRWLAEHRPATDLSGKQAVTAAKQFGTLGSGNHFVEVCL
                     DERDVVWVVLHSGSRGIGNQLAQMHIASARRLAKDLELRLEDPDLAYFLEGTPEFDGY
                     IADMLWAQDYARANRDQMMDRALREVLAFFGVGRERQRINCHHNFTQRERHGDADLWI
                     TRKGAIKADVGDLGIIPGSMGTRSYIVAGLGNPDSWTSCSHGAGRRLSRTQAKKQYTA
                     ADLTRKMAGKVWLSDRAASLVDEIPSAYKNIDQVMADQADLVEVRHTLRQVLNYKGT"
BASE COUNT         1006 a         2139 c         2172 g          873 t
ORIGIN      
        1 ccggcgatga ggtcggcctt cagcgtccag gaggcgttcg tgtagccgat cgccaacgcg
       61 aggttcggga cgtcctcgat catcatgccc ttgtagacca agcgcgaact ggggtcgacc
      121 ttctcaccgt cgacgaacag ctcgatgcct ccgaggaaga ggacgtcgag gccggtcgcc
      181 gtgacgatga tgtcggcctc gagctcctga cccgacgaga ggcgaatgcc cgtcggggtg
      241 aaccggtcga tgtgatcggt gacgaccgag gccgaaccgg aacggatcgc ccggaagagg
      301 tcgccgttgg gcacgacgca cagccgctgg tcccacgggt cgtacttggg ggtgaagtgg
      361 gtgtcgacgt cgtagccggc gggcagttgg cgttcgacgc ccttccgcag gatccgcttc
      421 atgagctcgg gacggcgccg gctcagctgg taggagccct gcgtggccag cgcgttcatc
      481 cacttgagca ccgggccctc gtagcgggcc ggcacgatct tgcgcaccag tgcggtgagg
      541 gggttcttgc cgggcaacga tgcgacatag gtcggcgacc gctgcagcat cgtcacgtgg
      601 cccgccgtcg gcgcgagcga ggggatcaac gtgaccgcgg tggcgccgct gccgatcacc
      661 acgatgcgct ggtcgcggta gtcgaggtcc tcgggccact tctgggggtg gacgatcgtg
      721 ccgccgaagt cgtccatgcc ctcgaactcg gggaggtagc cctggtcgta gcggtagtag
      781 ccgctgcagg agtacaggaa cgagcacgtg agctcgaacg tctcgccacc gccaccagcg
      841 gtgtcggtgc cgtcggtgcg ctcggcggtg acggtccaga gcgcggtgtc gctggagaac
      901 tcggcgcgga cgatgcggtg gcggaagcgg atcttgcggt cgatgccgaa ctccgacgcc
      961 gtgtccttga tgtactgcag gatggccccg ccgtcagcga tcgactccgg gcggttccac
     1021 ggccggaacg ggtagccgag cgtgaacatg tcggagtcgg aacggatgcc cgggtagcgg
     1081 aagaggtccc aggtgccgcc gatggcgtcg cgtgactcga acaacgcgta ggaggcccac
     1141 ggcgtctcgt tctgcacgtg gtaggcggcg ccgatgccgg acagcccggc gccgacgatg
     1201 agcacgtcga ggtgttcgac ctcgcccgtg gcgctcatgc cgcagagctc ccgcccggaa
     1261 gcagcccgac cagcgtgccc gtctcgccga agaggcggtc ccgccattcg tcggtgagcg
     1321 ccgtcgtgtc gcggtcgtcg gggtggaagt cggggcggtc gtagtcgcac aggtcgcgcc
     1381 acacctgttt ggtgacgagc ggcgaggtgc ggagcttctt gaggctccga cggaggttgc
     1441 cgtcgcggta cgccgccttg tcgccgagca ccgacagcat cacgctgatc gacatgccga
     1501 cgacgaaccc gaggcgagtc gccttcatcg tccacacccg gaggcgctcg ctgccgccca
     1561 cggccttgta cacgtcgaac gccacggcct tgtgctcgga ctcctcgagg gcatgccaca
     1621 ggaacacgtt ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcctggc
     1741 cgatgaaccc ggcgacctgg cgcttgagcg ccgggtcggt gatgcggtcg cggtagtggc
     1801 gcaccgaccg gacgaagaag tcttcgccgt cggggaacac ggccgacatg cagacggcga
     1861 ggtggctctc gatcaggtcg ccgtcggcgg cgaagtgctt cggcacgttg cgcagcgact
     1921 cctcgaacga gacccggcgg gtgggcacgt ggcgttcggg gttgctcgtc tggcgagggg
     1981 catcggtgat cgtcatgggg gttcctccga ggtcgtggcg tcagcgtaga gcatttgaga
     2041 gatttcctca atctctcatt tctggtagga tgtcacacat gaccacggcc gccacgtcgc
     2101 cctcgtcgaa gggcgagctc acccggcagt cgatcctcga tgcagcgatc gtccggttcg
     2161 gacgcgacgg ctaccgcgcc acctccgttg ccgacatcgc ccgcgacgcc gcggtgtccg
     2221 ggaccgccgc ctacgcccac ttccccaaca aggaagcgct gttcctcgcc gccctcgacg
     2281 acgacgctgc cgccgtcatc agcgaaggtg tgctccacct cctcgacgac tccgtggcga
     2341 gcgccgagtg gcacgagacg ctcatcctca cgctcgttgc cgcggtcgac cggcatccgc
     2401 tcgcgcgccg ggtcatcgcc ggcctcgaac cggacgtgac cgatcgggtc atcgacatcc
     2461 cagcgatgct cgaactgcgc aagacggtgg cggcccgcct gcgcgccgac caggccgcgg
     2521 gccgggtgcg ccccgacatc gatgcgtcgg cgatcgccga cggtgccgtc gggatcatcg
     2581 tgtccctcct catggcggtc atccaactcg gcacggccgc cgtcggcgtc tacggcgagg
     2641 gcatctgggc cgtcttcacg gcagcgctcg atccgctgcc ggactgaacg ctcagatcgc
     2701 tccctcccgc accaggcgct gatacgccag ccagcccggc aggagcggga tccagaaggt
     2761 ggcgagacgg aacagcagga ccgagcccag tgccatgtcc gccttcatgc cggcagcggt
     2821 cagaccggcg atgagggtgg cctcgaccgc cccgagcccg cccggcgtcg gagccgcgac
     2881 cgacacgacc gaggccagca ggtacacgag tgcgatggtg atgatcggca gggtcgaacc
     2941 gaacgcgtgc gccgacgccg cgagcgcgac cacatacccg agcgtcacgc ccgccgaacc
     3001 accgagcagc tcggccacgt tcgtcggcga gtgggcgacg aacgacaggc cgtgcagcgc
     3061 ccgctggacc ggcggtacca cgtgcagact ggcgagccga cggagggtcg gcaccgcgag
     3121 cgagacgagg accgtggtga cgatcgccgc caccacgatg aggatcgtcg acgccgacgg
     3181 gagcgtgaag cgctcgagga gatcggtgcc gccgagcccg aggaagacgc ccaacaagcc
     3241 gaggtggacg accacgcccg ccagggagtt gatgctcacc gctgcaaccg agacggcggc
     3301 gtcgatcccg cgcttgtgca ggaaccgggc gctgagggcg aggcttgcag cggtcgccgc
     3361 aaccgcgacg aacgacgtcg ccagctgcac ctcgacgttc ggtccgagtg ggatcggttc
     3421 cggcagtgag ccggcgaagg cgaccgcggc cgcgacgtag gtgagggccg agaccgcgag
     3481 ggcggccagc gcccaccccc agttcgcgtt gcgcacgagg ccccagacat cgccggcacc
     3541 gatcaactgg ggcacgagcg cccacgccgc caccgcggag gtggcaagga ggaagatcgt
     3601 gctgcccttg acccgctgca gacgttcgag cggcacgtcg gggaccccgg tgctgcgggt
     3661 cacctccgcc gtcagctcgg gcagcacgcc ggaccgcttg accaacgccc gagtcggcgg
     3721 cgtgagggcg agcggctgca accgcggcgc agccgccgtg acggcgtcga tcccgacggc
     3781 catgatcgcg gcgcgcaccg aggccgaggc gccgaaccgt gccgccgtcg cggcgagcac
     3841 ctcggccacg tcggcgttca acatgcgctc gggggcgccg agttccgcag actcgaagtc
     3901 gacgatccgc acgtggccgt cgccgtcgag cagcatgtgg tcgaacgaca gtcggtggtg
     3961 cgcggtgcga ctcgcccgca gggcggcggt actccgccag atctcggtca ggagcatcgg
     4021 ggtccactcg tgatcatcga gctgcgtgag cggacggacg tcgaacatct cgtaggcgag
     4081 cagccgcgac gccgtcccga cgttggtcga cgcgacgagc cgcgccgtcg gcacaccgtc
     4141 ggcgaacgcc ttgagcgcac cgagcgcctc gcggtccatc gggtgcttca ccgacccgaa
     4201 cgggcggtcc tcgcccacgt cgcggaaccg gatccagtgg gtcagtcgtg cgagacgccg
     4261 cgacttccac gactcccggg tcagcacctt gatgaacacg ccgcggccac cggcgtcgga
     4321 agcgaccatg gccacgtagc ccggcgcgag gcccgagaca tcggcgatgc tcacggcgtc
     4381 gaacccatga tcggacaaca cggcagcgac gtcctcgcat gccgggcgca ggttcggcat
     4441 accacccacg gcgaggatcg cgtggccgac gacccatccg actgcacacg cgaccaggac
     4501 ggtcgccggt tcggccaacg tgctcacgat gcgggccagg gcggcagcaa ccaccagtgc
     4561 gtacccggac cgacgcatcc gcgccgacca ccacggcgag tcgacgacga ccgcgctcac
     4621 gatggccgcg atgccgatca gcgacgggaa cccgagaccg acgccgaacc acacgactgc
     4681 aggacgggcg acatggaagt tgccctccgc gctgacggca ccaatggcgg ccatcgacgc
     4741 acccgagagc gccgctccca gcagcactcc accgacacgc tcgaagcgtc cggtggccaa
     4801 caacgcgatc agtcccacga acgcgctcag accgaccccg atctgggcga cggtcacgga
     4861 ggtgtcgacg acccattccg gggccgatcg caggagcgga ccggcgttgg cccggaactg
     4921 cgagtggtct ccaccggtcg cggtcacgag cgaccacgac gcggccagtg ccagcaccgc
     4981 gacgccgagc cgcaccggcg tcatcggcga ccgggcgcgc cgcggcaccg acgccgtcgg
     5041 ggggaggacg aggaccgaca tgagcgcaag ctcagcgggt ccacgcctcc cccgcaacta
     5101 ccagctcccc ggaatcaggt ccccttgtag ttgaggacct gccggagcgt gtgacgaacc
     5161 tcgacgaggt cggcctgatc ggccatcacc tggtcgatgt tcttgtacgc cgacgggatc
     5221 tcgtcgacga gcgacgcggc ccggtccgac aaccacacct tgccggccat cttccgtgtc
     5281 aggtcggcag ctgtgtactg cttcttggcc tgggtacggc tgagccgacg gccggcgccg
     5341 tgcgagcacg acgtccagct gtcggggttg ccgaggccgg ccacgatgta gctccgcgtc
     5401 cccatcgacc ccgggatgat cccgagatcg ccgacatcgg ccttgatcgc acccttgcgg
     5461 gtgatccaca ggtcggcgtc gccgtggcgc tcgcgctggg tgaagttgtg atggcagttg
     5521 atgcgctgac gctcgcgacc gacaccgaag aacgccagca cctctcggag cgctcggtcc
     5581 atcatctggt cgcggttggc gcgggcgtag tcctgggccc agagcatgtc ggcgatgtag
     5641 ccgtcgaact cgggggtgcc ctcgaggaag taggcgaggt cggggtcctc gagccgcagc
     5701 tcgagatcct tcgccagccg ccgcgccgac gcgatgtgca tctgcgcgag ctggttgccg
     5761 atgccacggc tccccgagtg cagcacgacc cagacgacat cgcgctcgtc gaggcacacc
     5821 tcgacgaagt ggttgcccga acccaaggtc ccgaactgct tcgccgccgt cacggcctgc
     5881 ttgccggaca ggtccgtcgc cgggcgatgc tccgcgagcc accggtcggc gcgacggccg
     5941 acgtcgccgt gaccctggcc cattccggcc gggatcaccc gggcgatccg cgccaggagc
     6001 ggctcgagat cgtcgggcag ctggtcctgg tgcacgtcga gctcggcggc gatcatgccg
     6061 cagccgatgt cgaccccgac ggccgccggg atgacggcac cacgggtcgg gatcaccgat
     6121 ccgacagtgg cgccgatgcc cacgtgggcg tcgggcatga gggcgacgtg gccggcgatg
     6181 atcggcaggc gcgccgtctt ctcggcctgg cgcagggtgc cgggctcgat gtcgctcgcc
     6241 cacgagatga gctggtcgtt gatcacggtg cgcatgacgg caccctcctt
//
LOCUS       DAOSOA010000188         6222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12684_length_6222_cov_5.371493, whole genome shotgun sequence.
ACCESSION   DAOSOA010000188 DAOSOA010000000
VERSION     DAOSOA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12684_length_6222_cov_5.371493"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PKY13_11425"
     CDS             complement(<1..1324)
                     /locus_tag="PKY13_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT80592.1"
                     /translation="MTSTVTPASPGLHHNDSREVIPLFVLLAAHFAIGLLLIAAGGRL
                     GRRAFVAAGLVPLVAFVWGTIQLPDVLDGRVVRSAATWIPELSLSLDLRLDAFASIMF
                     LLVTGIGTLVLLYATRYFGAATPGLPRLAGLLLIFTGAMLGVVTASNLLVLFVAWELT
                     SISSYLLIGWDDTNPVARSSALQALLTTGAGGLALLGGFVLIGEAAGTYDIAALATRP
                     PTGTSVSVGLVLVLIGAFTKSGQFPFSAWLPGAMVAPTPISAFLHSATMVKAGVYLIA
                     RLAPTFAGYGVWRPLVLGVGLVTMLTGGYRALREHDLKSILAFGTVSQLGFMVVLFGA
                     GLPEATYAGVVVLVAHALFKAALFLIVGVIDHQAHTRDIRALGRFGPGWDGPRVAAII
                     AGASMAGIPLCFGFVAKEAAYTAFAEGEAGWSGLVLVGIVAGSMLTVAY"
     gene            complement(1370..2947)
                     /locus_tag="PKY13_11430"
     CDS             complement(1370..2947)
                     /locus_tag="PKY13_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOT80593.1"
                     /translation="MNPARTSLFGRGGKRIWRPIAAGIGLASLALVALPGVAGAQEVE
                     PLPITGQQVNLLWVVLGAILVIFMQAGFALVETGFCRAKHAAHVVSTNFAIFALGFVG
                     FFIVGFGFAFGGFSYAAFGMDTAVGDAFIGSGEWTFLYKGGFMLTDMVGPGGAGAAGL
                     GFFLYMVAFMDTVATIPTGSMAERWKWSSFMVWGLFCGAIYYPLFAAWTWGGGWLGQL
                     GNSMELGLGYVDFAGSGVVHAVGGVAALAGAIVIGPRIGKFNKDGSANVIPGHHIPMA
                     MLGTFILLFGWFGFNAASTFAVTDVQFATVAANTAIAGAFGAIANILFMMFTRDKKPD
                     PGMMANGMLAGLVAVTAPCAFIDPWAAAVIGIIAGILVVFSVEFVEKRGIDDAVGAIS
                     VHGVCGSFGVLAVGIFSNGTYGAGWNLTTKGDAAEAAGVTGIFGGDFGVGLGQLAAQA
                     IGVVVIWTVIFGIAFAFFKIQNAVTKGGIRSSEADEIEGLDRPEMGAMAYPEFTHQDV
                     TPWLEEEAHKHHTATPV"
     gene            3142..3348
                     /locus_tag="PKY13_11435"
     CDS             3142..3348
                     /locus_tag="PKY13_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80594.1"
                     /translation="MNTPSTEVNRTKIRTGLAIIGVLFLGCLIGAAVVSDPTGRIVLL
                     AVAAVSLARLGLLTRSLRRETRSA"
     gene            complement(3376..3903)
                     /locus_tag="PKY13_11440"
     CDS             complement(3376..3903)
                     /locus_tag="PKY13_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT80595.1"
                     /translation="MAAQMTQSMVPPSVSGVALVRWPDDTDHLDRLRSQGRPRLLLVD
                     PGSPAPYTTALDEDWIRLPASEEDLHARSVALAAKAALVATNVPTVSGDGRLVQRGSW
                     VSLSPIEEQIMAALVADFGELVPLTVLTGGVADHVLTPNAVRVHVMRLRKRIAPMGLA
                     IQTVHGRGYLLETSP"
     gene            4031..5314
                     /gene="nhaA"
                     /locus_tag="PKY13_11445"
     CDS             4031..5314
                     /gene="nhaA"
                     /locus_tag="PKY13_11445"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOT80596.1"
                     /translation="MPSPRSAPRIIRPLRDFLAAESAGAILLSIGAIAALVWANSPWS
                     AAYGSLLHTDAGLAVGSWNFSLDLHGWVNDGLMALFFLVVGLEIKRELVEGELASLRQ
                     AALPAVAALGGMVVPALLFVAITAGQRGSGAWGIPMATDIALALGVVTVLGDRVPSAA
                     KIFLLALAIVDDLGAIVVIAVVYSSGVRFAYLAGAAVCVLAVVALGRWRPAFTLGFVV
                     IGVVLWWFVHEAGLHATLAGVIMGFLTPTAPRVPEDLIDEEILADVSDISAAQETIDM
                     ARASVSRVEWLEHVLHPWTSLVIVPIFAFANAGLVLDGDAFGRLFDSRIALGVLVGLV
                     VGKPLGIVGAVWLGRRAGLQLPEGMTMRTVAGLSVVAGIGFTVSIFVADLALSDPHDA
                     ESAKLAVMGASLIAAGAGALVLRSRATARSEPAPR"
     gene            complement(5283..5906)
                     /locus_tag="PKY13_11450"
     CDS             complement(5283..5906)
                     /locus_tag="PKY13_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT80597.1"
                     /translation="MSVELITTDGIFSLDGEDFDVTNNIWIVGDDREVVVVDAAHDAA
                     AIVSAVNGRRVRQILLTHGHNDHINAAVDLSEAVDAPIALHPADTMLWDVVYPDRRPD
                     LDLSEGARFSVGGHELSVLHTPGHAPGCCCFHDSRTAVLFSGDTLFCGGPGATGRSFS
                     DRPTILHSIRERLLALPGHTVVHTGHGDSTTIEAERPLIEELVRTEL"
     gene            complement(5906..>6222)
                     /locus_tag="PKY13_11455"
     CDS             complement(5906..>6222)
                     /locus_tag="PKY13_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HOT80598.1"
                     /translation="YQQCFAARDLAGTVVLVGVPQPDMVLELPFQEVFGRGGSLKSSW
                     YGDCLPERDFPMLIDLYLQGRLDLDRFVSETIGLEDVEAAFHKMERGEVLRSVVTIEP
                     GA"
BASE COUNT         1055 a         2068 c         2197 g          902 t
ORIGIN      
        1 tgtaggcaac cgtgagcatc gagcccgcca cgatgccgac cagcacgaga ccggaccagc
       61 cggcctcccc ctccgcaaag gcggtgtagg cagcctcctt cgcgacgaag ccgaaacaga
      121 gcgggatgcc cgccatcgac gccccggcga tgatcgctgc gacgcggggg ccgtcccacc
      181 cggggccgaa ccgcccgagg gcacggatgt cacgggtgtg ggcctgatgg tcgatcacgc
      241 cgacgatcag gaacagggcg gccttgaaca gggcgtgcgc cacgagcaca acaacaccgg
      301 cgtaggtggc ctcgggcagc ccggccccga agagcaccac catgaagccc agctggctga
      361 cggtgccgaa cgccaggatg ctcttgagat cgtgctcccg gagggcgcgg tagccgccgg
      421 tgagcatcgt gaccagaccg acgccgagca ccagcggtcg ccagaccccg tatccggcga
      481 aggtgggagc cagccgggcg atcaggtaca cgccggcctt caccatcgtg gccgagtgga
      541 ggaacgcgct gatcggcgtg ggtgcgacca tcgcgccggg aagccacgcg gagaacggga
      601 actggccgga cttggtgaag gcgccgatca ggacgaggac cagcccgacg ctgaccgagg
      661 tgccggtcgg gggccgcgtc gccaacgccg cgatgtcgta ggtgccggct gcctcaccga
      721 tgaggacgaa cccaccgagc agtgcgaggc caccggcgcc cgtggtgagg agggcctgca
      781 gcgccgacga tcgggcgacg ggattggtgt cgtcccaccc gatgaggagg tacgacgaga
      841 tcgaggtgag ctcccaggcg acgaacagca cgaggaggtt ggacgccgtc acgacgccca
      901 acatggcgcc ggtgaagatc aggagcaggc cggccagccg agggaggccc ggggtggcag
      961 ctccgaagta ccgggtggca tagaggagaa cgagggtgcc gatgccggtg accagcagga
     1021 acatgatcga agcgaaggcg tcgagccgga ggtcgagcga gagcgacagt tccgggatcc
     1081 acgtggcggc gctgcgcacg acccggccgt cgagcacgtc cggaagctgg atggtgcccc
     1141 acacgaacgc gacgaggggg acgaggccgg cggcgacgaa cgcccggcgt ccgagacgac
     1201 caccggcggc gatcaggagg aggccgatcg cgaagtgcgc ggcgagcagg acgaacagcg
     1261 gaatcacctc ccgggaatcg ttgtggtgga gacccggcga cgccggggtg accgtactgg
     1321 tcaccccggc gccgccggtc agtgaacgag ctgaactggt ccgatcaggt cacaccgggg
     1381 tggcggtgtg gtgcttgtgc gcctcctcct cgagccacgg ggtgacgtcc tgatgggtga
     1441 actcggggta ggccatggca cccatctcgg gccggtcgag gccctcgatc tcgtcggcct
     1501 cggaggagcg gatgccgccc ttggtgacgg cgttctggat cttgaagaag gcgaaggcga
     1561 tgccgaagat gacggtccag atgacgacca cgccgatggc ctgggcggcc agctggccga
     1621 ggccgactcc gaagtccccg ccgaagatgc cggtcacacc ggcggcctcg gcagcgtcgc
     1681 ccttcgtcgt caggttccag ccggcaccat aggtgccgtt ggagaagatg ccgacggcga
     1741 gcacaccgaa cgaaccgcag acaccgtgga ccgagatggc gcccaccgcg tcgtcgatgc
     1801 cgcgcttctc gacgaactcg accgagaaga ccaccaggat gccggcgatg atgccgatca
     1861 cggcggcggc ccacgggtcg atgaaggcgc agggagccgt gacggcgacg aggccggcga
     1921 gcatgccgtt ggccatcatg ccgggatcgg gcttcttgtc acgggtgaac atcatgaaga
     1981 ggatgttggc gatggcaccg aacgcaccgg cgatggccgt gttggccgcc accgtggcga
     2041 actggacgtc ggtcacggcg aaggtggaag cagcgttgaa gccgaaccag ccgaacagga
     2101 ggatgaacgt gccgagcatg gccatcggga tgtggtgacc cgggatgacg ttggccgaac
     2161 cgtccttgtt gaacttgccg atgcgaggac cgatcacgat ggcaccggcg agagcggcga
     2221 caccgccgac ggcgtgcacc acgcccgaac cggcgaagtc gacgtagccc aagccgagct
     2281 ccatggagtt gccgagctgg ccgagccagc cgccgcccca ggtccacgct gcgaacagcg
     2341 ggtagtagat ggcgccgcag aagaggcccc acaccatgaa cgagctccac ttccaccgct
     2401 cagccatcga acccgtcggg atcgtggcga cggtgtccat gaaggccacc atgtagagga
     2461 agaagccgag gccagcggcg cctgcaccac ccggacccac catgtcggtg agcatgaacc
     2521 cgcccttgta caggaaggtc cactccccgg atccgatgaa ggcgtcaccg accgcggtat
     2581 ccatgccgaa ggcggcgtag ctgaacccgc cgaaggcgaa gccgaagccg acgatgaaga
     2641 agccgacgaa cccgagggcg aagatggcga agttggtcga caccacgtga gcggcatgct
     2701 tggcccggca gaagccggtc tcgaccaggg cgaaaccagc ctgcatgaag atcacgagga
     2761 tggctccgag gaccacccac aggaggttga cctgctgccc ggtgatcggg agcggctcga
     2821 cctcctgagc tccggcgacc cccggaagag cgaccagcgc cagcgacgcc agaccgatgc
     2881 cggcggcgat cgggcgccag atccgcttcc caccccttcc gaaaagtgat gtacgtgctg
     2941 ggttcatgct gtgagtgtcc tttcgaacgt cgtggttggg ccggggcagc gccgttgctc
     3001 cctaccgacg agggtgaagc taggtatgag gtgtcacagg tccgtgtcgc aaatgtgaac
     3061 accgcgtttc gggctgctcg caggggactt cacgccgaca tcacgcccga ccggccggtc
     3121 gcgtgctgtc agaatggggc catgaacacc ccttccaccg aagtgaaccg cacgaagatc
     3181 cgtaccggac tggcgatcat cggcgtgctg ttcctcggtt gcctcatcgg cgcagccgtg
     3241 gtcagcgatc cgacggggcg gatcgtcctc ctggccgtcg cagcggtctc gctcgcacga
     3301 ctgggactgc tgacgcggag tctccgacga gagacacgct ctgcctgagc tcgaatgccg
     3361 gccgccccgg acgcgtcagg gtgaggtctc gagcaggtag ccccggccgt gcacggtctg
     3421 gatcgccagg cccatcgggg cgatccgctt gcgcagacgc atgacgtgca cgcgcacggc
     3481 attgggcgtc aacacgtggt cggccactcc gccggtgagc accgtcaacg ggaccagctc
     3541 gccgaagtcg gcgaccaggg cggccatgat ctgctcttcg atcggcgaca gcgataccca
     3601 ggacccccgt tgcaccagcc gaccgtcgcc gctcaccgtc ggcacgttcg tggcgacgag
     3661 cgccgccttg gcggcgaggg ccacggaacg ggcgtggagg tcctcctcac tcgccggcag
     3721 ccggatccag tcctcgtcga gcgcggtcgt gtacggcgcc ggggaaccag ggtcgacgag
     3781 cagcaggcgg ggtcggccct gcgaccggag acgatcgagg tgatcggtgt cgtcgggcca
     3841 cctcaccagg gccacgccac tcacgctcgg ggggaccatc gactgggtca tctgtgccgc
     3901 cacgacacga agcctacatt atgtgagcat taagacaacg gggttctgtc ggtccggtcg
     3961 gctagtgtgg catccggagt gccgggaagc ctggtcggcg attcgatcac cgaccccggg
     4021 aatctgcacc gtgccctcac cccgttccgc tccgcgcatc atccgccccc tccgcgactt
     4081 cctggccgcg gagtccgccg gcgccattct gttgtcgatc ggcgcgatcg cggcactggt
     4141 ctgggccaac tcgccgtggt ccgcggcata cgggtcgctc ctgcacaccg acgccgggct
     4201 cgccgtcggg tcctggaact tctcactcga cctccacggc tgggtcaacg acggcctcat
     4261 ggcgctgttc ttcctcgtgg tcggactgga gatcaagcgc gagctcgtcg agggcgagct
     4321 cgcgtcgctc cgacaggcgg cgctgcccgc ggtggccgcc ctcggcggca tggtcgttcc
     4381 ggctctcctg ttcgtcgcca tcaccgccgg ccagcgcggc tcgggcgcgt gggggatccc
     4441 gatggccacc gacatcgcgc tcgccctcgg cgtggtcacg gtgctcggcg accgcgtgcc
     4501 gagcgctgcg aagatcttcc tcctcgcgct cgccatcgtc gatgacctcg gcgcgatcgt
     4561 tgtcatcgcc gtcgtgtaca gctccggcgt acggttcgcc tacctcgcgg gggcggccgt
     4621 gtgcgtgttg gccgtcgtgg cactcggacg gtggcggccc gcgttcaccc tcgggttcgt
     4681 cgtgatcggc gttgtgttgt ggtggttcgt ccacgaggcg gggctgcacg ccaccctcgc
     4741 cggcgtgatc atgggattcc tgacacccac cgcgccgagg gttcccgagg acctgatcga
     4801 cgaggagatc ctcgccgacg tgtccgacat cagcgccgcc caggagacga tcgacatggc
     4861 gcgggcgtcg gtgtcgcggg tcgagtggct ggagcacgtg ctgcacccgt ggacgagcct
     4921 cgtgatcgtg ccgatcttcg cgttcgccaa cgctgggctc gtgctcgacg gcgatgcgtt
     4981 cggccggctg ttcgactccc ggatcgcgct cggtgtcctc gtcggcctcg tcgtcggcaa
     5041 gccgttgggc atcgtcggcg cagtgtggct gggccgtcgc gccggtctcc agctccccga
     5101 gggcatgacc atgcggacgg tcgccgggct cagcgtcgtg gccggcatcg ggttcaccgt
     5161 gtcgatcttc gtcgccgacc tcgccctctc ggacccacac gacgccgagt cggccaagct
     5221 cgccgtgatg ggggcgtcgc tgatcgccgc cggcgccggc gctctcgtgc tccgctcgcg
     5281 agctacagct cggtccgaac cagctcctcg atgagcggcc gctcggcctc gatcgtggtc
     5341 gagtcgccgt ggccggtgtg cacgaccgtg tgacccggta gggccagcag tcgctcgcgg
     5401 atcgagtgga ggatggtcgg ccggtccgag aagctgcgac ccgtggcgcc cgggccgccg
     5461 cagaacaggg tgtcaccact gaagaggacg gcggtgcggg aatcgtggaa gcagcagcac
     5521 ccgggcgcgt gacccggcgt gtggagcacc gacagctcgt ggccgcccac cgagaaccgc
     5581 gccccctcgg acagatcgag atcggggcgg cggtcggggt agacgacgtc ccacagcatc
     5641 gtgtccgcgg ggtgcagggc gatcggggcg tcgaccgctt cggacaggtc gaccgcagcg
     5701 ttgatgtggt cgttgtgacc gtgggtcagg aggatctgcc gcacacggcg gccgttgacc
     5761 gcgctgacga tcgcggccgc gtcgtgcgcg gcgtcgacga ccacgacctc gcggtcgtca
     5821 ccgacgatcc agatgttgtt ggtgacgtcg aagtcctcgc cgtcgaggct gaagatgccg
     5881 tcggtggtga tcagctcgac cgacatcagg cacccggctc gatcgtcacc accgaacgca
     5941 gcacctcgcc gcgctccatc ttgtggaagg ccgcctcgac gtcctccagc ccgatcgtct
     6001 ccgacacgaa gcggtcgaga tcgaggcggc cctgcaggta gaggtcgatc agcatcggga
     6061 aatcgcgttc gggcaggcaa tcgccgtacc acgagctctt gagcgacccg ccccgaccga
     6121 acacctcctg gaagggcaac tcgagcacca tgtcgggctg cggaacgccg acgagtacca
     6181 cggtgccggc gaggtcgcgg gcggcgaagc actgctggta ca
//
LOCUS       DAOSOA010000189         6209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12722_length_6209_cov_5.480338, whole genome shotgun sequence.
ACCESSION   DAOSOA010000189 DAOSOA010000000
VERSION     DAOSOA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12722_length_6209_cov_5.480338"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2853)
                     /locus_tag="PKY13_11460"
     CDS             complement(1..2853)
                     /locus_tag="PKY13_11460"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT80599.1"
                     /translation="MALPLRLPTPIAERRGAKPVIHAIEVVALVAGAGALAFLSSDQT
                     MLIVIGVLFIVVAAFALVLSRPSVVVEVAVITMWFDSVGAGPVRTGRVVSGLAIFVLF
                     ARLAASDWKPPALLPRAWVMPGLFFMWAWVSGFWSNEMGLWIQGLLELTLGFIYALII
                     MLFIENEEHLARSFKAWVWTGVPIAIISYVLFNKIEEVQADIGGENRIVGFTGNANAY
                     ATLLAVAVPVTVVFMRRAKSRAERLVYAACIFGFMVALVTTGSRGGLITMGMIVVYVF
                     VTYPGLDRRQRVKSSVGGIVFVATGVFLAGIMNPDRYSLIGFFGDAGAGRLDLWNAAT
                     KSFSLQPIQGFGIGGFRTQMLDVLTKVPGGSLDITKPIANRSAAGLEVHNTYLTILLD
                     LGVIGLVLYMVTMLSVFKNLWDLRQTQWRDWAWAAFGCNGAMVAGAFFGSGYNLKFQW
                     TVVGLAGAGFVRQRVTGRGDRVRAHRGLPALRESVRPPDPGVSAVQGERAFAAPMDLR
                     LRYPFRWVMVAAILLGLFGGIFGAKAFGSPTYTTYGRVLVINLDNNDPRFGVQITDSR
                     IQFVLNIARSERYLAEVQRRAGLTDSLDQLAEEIDSTRPGFSPVIRITAKTGDPEKTK
                     RIGAVLIDALDSVVDSARSGAMVVVGEDGRSVSPDTDPDYRGPLYLHLFDESITSESA
                     PRTIYCGFLGAGVAGLGLVMVAMLLHGRRRLSSDEDIPDILGIPFVASMPRPLLGRSK
                     NAPAIYRSVGDQIDDACAAPPRVIALCSNDLSTLQSRIALGAACGLAIVEPQPIVVLD
                     LDIGSRGLSRQLGMNRRDGIIDVALGTTDLASVRRALPRWRIPRAFRRLTAADKQRIT
                     VVPVGGRRVLRDQGTVLDETAVSDLIAALSTEALVVVNLPPVPGPLPVREILLQCDAV
                     LVTVLDGWSEVDAAQTTIDALEAAVPNRVGYLLVDQ"
     gene            complement(2968..3261)
                     /locus_tag="PKY13_11465"
     CDS             complement(2968..3261)
                     /locus_tag="PKY13_11465"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HOT80600.1"
                     /translation="MRIERREGYTLLVGGPVPRGAAAITIGSVISIRERCLDDGHLLR
                     HELVHVRQWRELGVVRFGAMYLSSYVRSRWDGYGHWAAYRRIPLEIEAEWEAR"
     gene            complement(3264..3971)
                     /locus_tag="PKY13_11470"
     CDS             complement(3264..3971)
                     /locus_tag="PKY13_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT80601.1"
                     /translation="MVDVTDATFEQEVMARSMQVPVVIDLWATWCQPCVALGPIIERA
                     VAATNGQVVLAKVDVDANPQIGAAFRVQSIPAVFAMHQQKIVDQFLGAQPEAFVTEFV
                     NKLLPTKEETEVERLLSLGDEASLRAALELEPGNEVVTCALAELLVMAGETDEAQVLL
                     AKIPETAEVRRVLAMARTGLSDASALDVEGELTSLLDRVKDDDDARQRYVDLLAVLGP
                     DDPRTADYRRKLTTRLF"
     gene            4047..5090
                     /locus_tag="PKY13_11475"
     CDS             4047..5090
                     /locus_tag="PKY13_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HOT80602.1"
                     /translation="MEAPKGIAVDEVTSWLTERVPGCTPPLDFSLIAGGHSNLTYTVT
                     DQADGTWVLRRPPLGHVLATAHDMSREHTIISALQGTAVPVPPVVGLCTDEAVNGAPF
                     YVMGYVDGTVVRDMATADSLSVDQRRRASDSLVEVMAAIHNIDVEAVGLADLGRHDGY
                     IERQLKRWYRQYQGSQEQNSSIDFKQVDEVYEHLSARIPEQQGTSIVHGDYRLDNCMI
                     GADGNIVAVLDWEICTLGDPLADVGLLWVYWTDPGTDAVLPQSSPTALEGFRTKDEMI
                     AAYAAASGRDVSQIDFYIAFGYWKLTCIIAGVYARYAAGAMGDTSDQQVQGFAHMVAT
                     LAQLAADAAEKVV"
     gene            5091..5957
                     /locus_tag="PKY13_11480"
     CDS             5091..5957
                     /locus_tag="PKY13_11480"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOT80603.1"
                     /translation="MSELVELHGHPPLSSPVMILALEGWIDAGMAANGAMTAIIEGVD
                     TEPLASFDTDVLLDHRARRPMMSLVEGHVEQLTWPSIEVRGGIDDAGNHVVLVVGAEP
                     DHAWGRFCTAVTDLALDLDVRMVIGLGAYPAPVPHTRDTNLALTSPSTELLDSHTGFF
                     RGTIDVPAGIQSAIEMSTHDVGISSMALWAQVPHYISGMNYPAGSLALVEGLGRVAGL
                     SFPTASLALEATSTRHRLDELVSGNRQHEEMLRQLEEVADAAPAVDDLGPIPSGDEIA
                     DEFQKFLRDQER"
BASE COUNT         1088 a         2121 c         2124 g          876 t
ORIGIN      
        1 ctactggtcg acgagcaggt agccgacccg gttcggcacc gccgcctcga gcgcatcgat
       61 cgtcgtctgc gccgcgtcga cctccgacca gccgtcgagc acggtgacga gtaccgcgtc
      121 gcactggagg aggatctcgc ggaccgggag cgggcccggg accggcggca ggttgacgac
      181 cacgagcgcc tccgtcgaca acgcggcgat caggtcggat accgccgtct cgtcgagcac
      241 cgtgccctga tcccggagga cccgacgacc gccaaccggc acgaccgtga tgcgctgctt
      301 gtcggcggca gtcaggcggc ggaacgcccg cgggatacgc caacggggca acgcacgacg
      361 caccgacgcg aggtccgtcg tgccgagcgc cacgtcgatg atgccgtcgc gccggttcat
      421 gccgagctgg cggctgagac cccgcgaccc gatgtcgagg tcgaggacca cgatcggttg
      481 gggctcgacg atggcgaggc cgcacgcggc gccgagcgcg atgcgcgact gcagcgtcga
      541 gaggtcgttg ctgcacagcg cgatgacccg gggcggtgcg gcgcacgcgt cgtcgatctg
      601 gtcgccgacg gatcgataga tggcgggggc gttcttggat cgaccgagca gcggacgggg
      661 catcgacgcg acgaagggga tgcccaagat gtcggggatg tcctcgtccg acgacaggcg
      721 tcggcgaccg tgcaacagca tcgccaccat cacgaggccc aggcccgcga caccggcgcc
      781 gaggaacccg cagtagatgg tccgcggagc cgactccgac gtgatcgatt cgtcgaacag
      841 gtgcaggtac agcgggccgc ggtaatcggg gtcggtgtcg ggcgagaccg atcggccgtc
      901 ctcgccgacg acgaccatcg cacccgagcg ggcggagtcg accaccgagt cgagcgcgtc
      961 gatgagcacc gcgccgatcc gcttggtctt ctcgggatcg cccgtcttcg ccgtgatgcg
     1021 gatgaccggg gagaagcccg gacgggtcga gtcgatctcc tccgccaact ggtcgaggga
     1081 gtcggtcaac ccggcacgac gctgcacctc ggcgaggtac cgctccgacc gggcgatgtt
     1141 gagcacgaac tggattcggc tgtcggtgat ctgcacaccg aagcgcggat cgttgttgtc
     1201 gaggttgatg acgagcacac ggccatacgt ggtgtacgtc ggcgagccga aggccttggc
     1261 accgaagatg ccgccgaaca ggccgagcag gatcgcagcg accatcaccc accggaacgg
     1321 gtaccgcagg cggaggtcca tcggcgccgc gaaggcacgc tccccctgca ccgcgctcac
     1381 acccgggtcg ggtgggcgca ccgactcgcg gagcgccggc agaccacggt gcgcacggac
     1441 ccggtcgcca cgaccggtga cgcgttgccg gacgaagccg gccccagcga gaccgacgac
     1501 ggtccactgg aacttcaggt tgtaaccgga tccgaagaag gcgccggcca ccatcgcgcc
     1561 gttgcagccg aacgccgccc aggcccagtc acgccattgc gtctggcgaa ggtcccacag
     1621 gttcttgaac acgctcagca tcgtgaccat gtagagcacg agaccgatga cgccgaggtc
     1681 gaggaggatc gtgagatagg tgttgtgcac ctcgaggccg gcggcgctgc ggttggcgat
     1741 cggcttggtg atgtcgagcg acccgccggg caccttggtc agcacgtcga gcatctgggt
     1801 gcggaaccca ccgatgccga acccctggat cggctgcagc gagaacgact tggtcgcggc
     1861 gttccacagg tcgaggcggc cggcgccggc gtcgccgaag aagccgatca gggagtagcg
     1921 atcggggttc atgatgccgg cgaggaacac acccgtggcc acgaagacga tgccaccgac
     1981 cgaactcttg acgcgctgcc ggcggtcgag gccgggatag gtgacgaaga cgtacaccac
     2041 gatcatgccc atcgtgatga ggccaccgcg ggagccggtg gtgacgagcg cgaccatgaa
     2101 accgaagatg caggcggcgt acaccaggcg ctcggcgcgg gacttcgccc gcctcatgaa
     2161 caccaccgtg accgggacgg cgaccgcgag cagcgtggca taggcgttcg cgttgccggt
     2221 gaagccgacg atgcggttct caccgccgat gtccgcctgc acctcctcga tcttgttgaa
     2281 gaggacgtag gagatgatgg cgatgggcac gccggtccac acccacgcct tgaacgagcg
     2341 ggccagatgc tcctcgttct cgatgaacag catgatgatc agggcgtaga tgaacccgag
     2401 cgtgagctcg aggaggccct ggatccacaa gcccatctcg ttcgaccaga acccgctgac
     2461 ccaggcccac atgaagaaca gtccgggcat gacccacgcc cgcggcagga gcgccggtgg
     2521 cttccagtcc gatgcggcca gtcgagcgaa caacacgaag atcgcgagcc ccgagacgac
     2581 gcgcccggtg cggaccggcc cggcgccgac cgagtcgaac cacatcgtga tgaccgccac
     2641 ctcgaccacg accgacggac ggctgagcac aagtgcgaac gccgcgacga cgatgaagag
     2701 caccccgatg acgatcaaca tcgtctgatc ggagctgagg aatgccagcg ccccggcgcc
     2761 ggcgacgagc gccaccacct cgatggcgtg gatcaccggc ttggccccac ggcgttcggc
     2821 gatcggggtc gggagtcgga gtgggagcgc catggttcag ggtcgtttca cggcgcggct
     2881 gtcggttccc cgcagagcgc aacggatgaa cgttaccggt ggacctgcgt cggcgggaag
     2941 cacaccgcca gagccttcac cgttgtttca cctcgcctcc cactccgcct cgatctcgag
     3001 gggaatgcgg cggtaggcgg cccaatggcc gtacccatcc caacgggacc ggacgtagga
     3061 ggacaggtac atcgcaccga agcggacgac gccgagctcg cgccactggc gtacgtggac
     3121 cagttcgtgg cggagcaggt ggccatcgtc gaggcagcgc tcgcggatgg agatcaccga
     3181 accgatcgtg atggcagcgg caccccgcgg caccggaccg cccacgagga gggtgtagcc
     3241 ctcgcgccgc tcgatcctca aggtcagaac agccgggtgg tgagcttgcg acggtagtcg
     3301 gcagtgcggg gatcgtcggg gccgagcacg gcgagcaggt cgacgtagcg ctgccgggcg
     3361 tcgtcgtcgt ccttcacccg atcgagcaac gacgtgagct caccctcgac atcgagcgcc
     3421 gacgcgtcgc tgaggccggt ccgtgccatc gccagcacac gacgcacttc ggcggtctcg
     3481 gggatcttcg ccaggagtac ctgggcctcg tcggtctcgc ccgccatcac gagcagttcg
     3541 gcgagcgcac aggtgaccac ctcgttgccc ggctcgagct cgagcgcggc ccgcaacgac
     3601 gcctcgtcgc cgagcgagag cagacgctcg acctcggtct cctccttggt cggcaggagc
     3661 ttgttgacga actcggtgac gaacgcctca ggctgggcgc cgaggaactg atcgacgatc
     3721 ttctgctggt gcatggcgaa caccgccggg atcgactgca cacgaaacgc ggcgccgatc
     3781 tgggggttgg cgtcgacgtc gaccttggcc agcaccacct ggccgttggt cgcagcgacg
     3841 gcgcgttcga tgatcggacc caacgcgaca cacggctggc accaggtggc ccacaggtcg
     3901 atgacgaccg ggacctgcat ggagcgcgcc atgacctcct gctcgaacgt ggcatcggtg
     3961 acatcgacca tgggcacaga tcctagaggg caggctcccg gcggaacgcg ccgggaccga
     4021 cccggcccgg gaggtagcgt gcggggatgg aagctccaaa gggaatcgcc gtcgacgagg
     4081 tcacctcgtg gctgaccgaa cgcgtccccg gttgcacacc cccgctcgac ttctccctca
     4141 tcgccggcgg ccactccaac ctcacctaca cggtcaccga ccaggcggac ggcacgtggg
     4201 tcctccgccg cccgccgctc gggcacgtgc tcgccacggc ccacgacatg agccgcgagc
     4261 acacgatcat ctcggcgctg cagggcacgg ccgtccccgt gccaccggtc gtcggcctgt
     4321 gcaccgacga ggcggtcaac ggcgcgccgt tctacgtcat gggctacgtc gacggcacgg
     4381 tcgtccgcga catggcgacc gccgactcgc tcagcgtcga ccagcgccgt cgagcgtccg
     4441 actcgctcgt cgaggtcatg gccgcgatcc acaacatcga cgtcgaggca gtggggctgg
     4501 ccgacctcgg acggcacgac ggctacatcg aacgccagtt gaagcggtgg taccggcagt
     4561 accagggcag ccaggagcag aactcctcga tcgacttcaa gcaggtcgac gaggtgtacg
     4621 agcacctgtc ggcgcggatc cccgaacagc agggcacctc gatcgtccac ggcgactacc
     4681 gcctcgacaa ctgcatgatc ggtgccgacg gcaacatcgt tgcggtcctc gattgggaga
     4741 tctgcacact cggcgacccg ctcgccgatg tcggcctgct gtgggtgtac tggaccgacc
     4801 ctggcaccga cgccgtgctc ccccagtcgt cgccgaccgc actcgagggg ttccgcacga
     4861 aggacgagat gatcgcggcc tacgccgcgg cgtcggggcg cgatgtctcc cagatcgact
     4921 tctacatcgc gttcggttac tggaagctca cgtgcatcat cgccggcgtg tacgcgcggt
     4981 acgccgccgg ggcgatgggc gacacgagcg accaacaggt ccaggggttc gcccacatgg
     5041 tcgccaccct ggcgcagctg gccgccgatg cggccgagaa ggtggtctga atgtcagaac
     5101 tcgtcgaact ccacggtcac ccgcccctgt cgtcgccggt catgatcctc gccctcgaag
     5161 gctggatcga cgccggtatg gcggccaacg gcgccatgac cgcgatcatc gagggcgtcg
     5221 acaccgagcc gctggcgtcg ttcgacaccg acgtgctgct cgaccaccgg gcccggcgcc
     5281 cgatgatgag cctcgtcgag ggtcacgtcg agcagctcac gtggccgtcg atcgaggtgc
     5341 gcggcggcat cgacgatgcc ggcaaccacg tcgtgctcgt ggtgggtgcc gagcccgatc
     5401 acgcatgggg caggttctgc accgcggtga ccgacctggc cctcgacctc gacgtgcgga
     5461 tggtcatcgg cctcggggcc tacccggcgc cggtgccgca cacccgcgac accaatctgg
     5521 cgctcacgag cccgtcgacc gagctcctcg acagtcacac cgggttcttc cggggcacca
     5581 tcgacgtccc cgccggcatc cagtcggcga tcgagatgag cacgcacgac gtcgggatct
     5641 cgtcgatggc gttgtgggcg caggtgccgc actacatctc gggcatgaac tacccggcag
     5701 ggtcgctggc cctcgtcgag gggctcggcc gggtggccgg cctgtcgttc ccgacggcct
     5761 cgctggcact cgaggccacg agcacgcggc accgcctcga cgagctggtg tcgggcaacc
     5821 gccagcacga ggagatgctc cgccagctcg aagaggtcgc cgatgccgcc ccggcggtcg
     5881 acgatctcgg cccgatcccg tcgggcgacg agatcgccga cgagttccag aagttcctcc
     5941 gcgaccaaga aagatagagc ccggcggagc cggtccgtca cgggcggcgt gggtgggtga
     6001 tagagcccac cggttctgtc ctgcggttcg gcccccacag gggcggaact gcaggacaga
     6061 acgggtgaga gacgggcgct accgtttcag cggctcgtac tcgggcagct cggagccctt
     6121 cggcgcgtcc tcgtagatga tcacgctgcc ctcctcctcg aagagcttgg actcggcgtc
     6181 ggcgtcccac tcccccggca gcacccgct
//
LOCUS       DAOSOA010000190         6144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12882_length_6144_cov_6.001642, whole genome shotgun sequence.
ACCESSION   DAOSOA010000190 DAOSOA010000000
VERSION     DAOSOA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12882_length_6144_cov_6.001642"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PKY13_11485"
     CDS             <1..1114
                     /locus_tag="PKY13_11485"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT80604.1"
                     /translation="QHNVEVRMARVTDIDLDANAITLDSGATVPYDTLVIAAGAVSTS
                     FGVPGVDEHGLPLKSLDDAIAIRRRILDQFERAAADPSLVDDGALNVVVCGGGPTGVE
                     MAGGIMELFNKVLQHDFPDLPVRWARVVLVEAADRLLPPFSEQSSRRALRTLTRRRVE
                     VHVGVGVERVEPTRVHLADGTVVPAHTVIWAAGVAASPLAKLVGTETTRGGRLVVGDD
                     LSIPGHPEVFAIGDIAATSTPLPQVAQPAIQGGHHVAAQIRRRLSGQATEPFRYVDKG
                     SMATIGRLDAVADLPGGIHLTGPIGWVAWLGLHLLYLMGFRNRINVFVNWCWNYLTYD
                     RGSRLLAESQRIAAEAPDHLRGSGDGPRSGATSPGE"
     gene            1133..2149
                     /locus_tag="PKY13_11490"
     CDS             1133..2149
                     /locus_tag="PKY13_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOT80605.1"
                     /translation="MSETATYVEPGEFQRDSAYIETRITADGSDGFPVEPGRYRLIVA
                     RACPWANRAVIVRRLLGLEPVLSMGMCGPTHDARSWTFDLDPDGRDPVLGYERLQEAF
                     FARDPEYAKGITVPAIVDIPTGAVVTNDYPQITLDLSTEWTAFHREGAPDLYPVSHRD
                     EIDDVADLVFRDVNNGVYRCGFAGGQSAYDAAYRRLFERLDWLTDRLADRRYLVGDTI
                     TEADVRLFTTLARFDAVYHGHFKCNRSKLSEMPVLWAYARDLFQTPGFGDTTDFVQIK
                     QHYYIVHRDINPTGIVPAGPDLSGWLTPHGRESLGGRPFGDGTPPGPPIPAEVVPAAH
                     GAGG"
     gene            2173..3471
                     /locus_tag="PKY13_11495"
     CDS             2173..3471
                     /locus_tag="PKY13_11495"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HOT80606.1"
                     /translation="MTRGHHGTGWRGALGIGVLAATVVLGACSDDDSTDSGNGPETTT
                     TTTAPPAVSSTTTPTPGPLDRYAGYQSVNYADDTRWLCRPDRTDICSGNLDTTTIAAD
                     GTLTVVPFEPAADPAIDCFYVYPTISRDPTPNSDWDASDAEEGYAAINQVARLRAGCR
                     VFAPIYRQRTLGALASNLGSGDSPGEVVDPFGDVLDAWKTYMATENRGRGVVLVGHSQ
                     GAALLNQLIQEEIDPNDDVRSLLVSAYLAGWAVAVPEGEDVGGDFAKVPLCRSDDQAG
                     CVVTWSSFRSSAPPVPGAFFGKPRTGDDPAACTNPAALAGGSAEPDSVFPANASASIL
                     ASVAADAPGAGVWVDPALGKVTTPFVSVPGLVSTECASTDGFSYLKVTVQGDPADPRA
                     DDIGGDLTPEWGLHLIDFNLVMGNVVDLVATQATSWASAR"
     assembly_gap    3487..3586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3587..4237
                     /locus_tag="PKY13_11500"
     CDS             <3587..4237
                     /locus_tag="PKY13_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT80607.1"
                     /translation="GPMTRRPVRIAVLNDYEITVAGVAAMLAAFADRAVVVELSVGDP
                     TLDRQVDLALFDTYGRPDFAEVEISELVADDRVGCVVVFTNSIPPAEINRLLAIGVSG
                     CLSKSLSAERLVDELELIATGEQRISSFEDLANPVVVANAPGARWGLTYRESEVLALL
                     SLGMRNKEIAATLYVGGETVKSHLASIFRKMKVASRAEAISTAVRTGGFDRVADPR"
     gene            4469..5116
                     /locus_tag="PKY13_11505"
     CDS             4469..5116
                     /locus_tag="PKY13_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT80608.1"
                     /translation="MTEPIKIAILNDYELVVAGIRRMLEPHAGRVKVVETVVGSADVD
                     HEVDIALYDTFGRADVGVDRISALVANPRIGHVVIYTTSMPADATRQLLELGVSGCLS
                     KSLSAIDLVRSLERVHRGEVTIAMRSSGGPSDFSSPNLKLSYRESEVLALLSQGLRNR
                     AIAKALYVGEETVKSHLKSIYRKLGVSSRGEAIAVAFREPSFAEPKPLDDEAISG"
     gene            5224..>6144
                     /locus_tag="PKY13_11510"
     CDS             5224..>6144
                     /locus_tag="PKY13_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80609.1"
                     /translation="MPRNRPRPDAVPAPTDDEMAALAALGRNGEWELQGRTLKLTNLD
                     KVLFPAGRDRSGIDQPAFTKRDLVAHYARVGPVMVPYLRGRALNMNRFPNGVDQPGFW
                     HKAVPSHAPEWMQQWQYERASEGTTRWYFVVDSVPALAWMANFGVVEMHAWTSPITAP
                     DRPSYALIDLDPGTTTTFDDLVTLALLHRTALEHLGVTAQPKVTGQRGIQIWVPVEPG
                     PTFEDTRVWVEKVSRAVGAMVPDLVSWAWNTKERKGLARLDYTQNAVNKTLVAPYSVR
                     AAIGAPVSMPISWDELDDPDLRPDGWSIATA"
BASE COUNT          961 a         2097 c         2072 g          914 t
ORIGIN      
        1 acagcacaac gtcgaggtac ggatggcgcg ggtcaccgac atcgatctcg acgcgaacgc
       61 gatcaccctc gactcgggcg caacggtccc ctacgacacg ctcgtgatcg ccgcgggtgc
      121 cgtgtccacc agcttcggag tacccggggt cgacgagcac ggccttcccc tgaagtcgct
      181 cgacgacgcc atcgcgatcc gccggcgcat cctcgatcag ttcgagcggg ccgccgccga
      241 cccgtcgctc gtcgatgacg gcgcgctcaa cgtggtcgtg tgcggcggcg ggccgaccgg
      301 cgtcgagatg gcgggcggga tcatggagct cttcaacaag gtcctgcagc acgacttccc
      361 cgatctcccg gtgcggtggg cccgagtggt gctggtcgaa gcagccgatc ggctgctccc
      421 gccgttcagt gagcagtcgt cgcggcgagc attgcgaacg ttgacgcgcc ggcgggtcga
      481 ggtacacgtc ggggtgggtg tggaacgggt cgagcccacg cgggtccatc tcgccgacgg
      541 cacggtggtg ccggcgcaca ccgtcatctg ggccgccggc gtggcggcca gcccgttggc
      601 gaagctcgtc ggcaccgaga cgacacgcgg aggtcgactc gtcgtcggcg acgacctgtc
      661 gatcccggga catcccgagg tgttcgcgat cggcgacatc gccgcgacgt cgacgccgct
      721 cccccaggtg gcacagcccg ccatccaggg cggtcaccac gtggcggcgc agatccgccg
      781 acggttgagt ggtcaggcga ccgagccgtt ccgctacgtc gacaagggtt cgatggcgac
      841 gatcggccga ctggacgcgg tcgccgatct cccgggcggg atccacctca ctggtccgat
      901 cggctgggtg gcgtggctgg ggctgcacct cctctatctc atgggcttcc gcaaccgcat
      961 caacgtgttc gtgaactggt gttggaacta cctgacctac gaccgtggca gccgcctgct
     1021 cgccgaatcg cagcgcatcg cagcggaggc tcccgaccac ctgcgggggt caggggacgg
     1081 accacgttcc ggggcgacct ctcccggcga atgaagtcgt acaaacttga acatgtccga
     1141 aacagcaacg tacgtcgagc ccggtgagtt ccagcgggac tccgcttaca tcgagacccg
     1201 catcaccgcc gacggcagcg acggcttccc ggtcgagccg ggccgctacc gactgatcgt
     1261 cgcccgggcg tgcccctggg ccaaccgcgc cgtgatcgtg cgtcgcctgc tggggctcga
     1321 accggtgctg tcgatgggca tgtgcggccc gacccacgac gcccgcagct ggacgttcga
     1381 cctcgacccc gacggtcgcg acccggtgct gggctacgag cggttgcagg aggcgttctt
     1441 cgcccgcgac cccgagtacg cgaaggggat caccgtgccg gccatcgtcg acatcccgac
     1501 gggagccgtc gtgacgaacg actacccgca gatcaccctc gacctgtcga ccgagtggac
     1561 cgcattccat cgtgagggcg cacccgacct ctacccggtg tcgcatcgcg acgagatcga
     1621 cgacgtggca gacctcgtct tccgcgacgt caacaacggc gtgtaccgat gcggcttcgc
     1681 cggcgggcag tcggcgtacg acgccgcgta ccggcggctg ttcgagcggc tcgactggct
     1741 cacggaccgt ctcgccgacc gccggtacct cgtgggcgac acgatcaccg aggccgacgt
     1801 ccgcctgttc accacgcttg cccggttcga cgcggtctac cacgggcact tcaagtgcaa
     1861 tcgctcgaaa ctgtcggaga tgcccgtgct gtgggcgtac gcccgagacc tcttccagac
     1921 gccggggttc ggtgacacca ccgacttcgt ccagatcaag cagcactatt acatcgtcca
     1981 ccgcgacatc aacccgaccg gcatcgtgcc cgccgggccc gacctgtcgg gctggctgac
     2041 gccgcacggc cgcgagtccc tcggggggcg acccttcggc gacggcacac cacccggccc
     2101 accgatccct gccgaggtgg tgccggcggc ccacggcgcc gggggttgat ggcggcgact
     2161 accgtcgccg ccatgacacg gggacaccac gggacaggtt ggcggggcgc gctcggaatc
     2221 ggagtgttgg cggcgacggt ggtgttggga gcgtgttccg acgacgattc gaccgactcc
     2281 ggaaacgggc cggagacaac gacgacgacc acggcgccgc cggcggtctc gtccaccacc
     2341 acgccgacgc ccggcccact cgatcggtac gccggctacc agagcgtcaa ctacgccgac
     2401 gacacccgct ggttgtgccg gcccgaccgc accgacatct gctcgggcaa cctcgacacc
     2461 accacgatcg ccgccgacgg aacgctcacg gtcgtgccgt tcgagccggc agccgacccc
     2521 gccatcgact gcttctacgt gtacccgacg atctcccggg acccgactcc caacagcgac
     2581 tgggacgcgt ccgacgccga ggagggctac gccgcgatca accaggtggc gcggctgcga
     2641 gccggctgcc gggtgttcgc cccgatctac cgccagcgca ccctcggcgc cctcgcgagc
     2701 aatctcggtt ccggcgactc ccccggcgag gtggtcgatc cgttcggcga tgtgctcgac
     2761 gcgtggaaga cctacatggc gaccgagaac cggggccggg gtgtggtgct cgtcggccac
     2821 tcccagggcg cggcattgct gaaccaactg atccaggagg agatcgaccc gaacgacgac
     2881 gtacgttcgt tgctcgtgtc ggcctacctc gccgggtggg cggtggcggt gccggagggt
     2941 gaggacgtcg gcggcgactt cgcgaaggtc cccctctgtc ggtccgacga ccaggccggc
     3001 tgcgtcgtca cgtggtcgtc gttccggtcg agcgctccgc cggtgcccgg cgcattcttc
     3061 ggcaagcccc gcaccggcga cgaccccgcc gcgtgcacca acccggcggc gctcgccggc
     3121 ggctcggcgg agcccgacag cgtgttcccg gccaacgcca gcgcgtcgat cctggcatcg
     3181 gtggccgccg acgctcccgg cgccggcgtc tgggtcgacc cggcgctcgg caaggtgacg
     3241 acaccgttcg tgtcggtgcc gggcctggtg agcacggagt gcgcgagcac cgacgggttc
     3301 agctacctga aggtgacggt gcagggcgat ccggcggatc cccgcgccga cgacatcggc
     3361 ggcgacctca cgccggagtg gggccttcac ctcatcgact tcaacctggt catgggcaat
     3421 gtggtcgacc tcgtcgccac ccaggcgacc agctgggcct cggcccgctg atcccgaccg
     3481 cccgacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggc cgatgacgag
     3601 acgaccggtg cgaatcgcag tcctgaacga ctacgagatc accgttgccg gagtggcagc
     3661 gatgctcgcg gcgttcgccg atcgcgccgt ggtggtcgag ctgagcgtcg gcgatcccac
     3721 cctcgatcgg caggtcgacc tcgcgttgtt cgacacgtac ggtcggcccg acttcgccga
     3781 ggtcgagatc agcgaactgg tggctgacga tcgagtcggc tgcgtcgtgg tgttcacgaa
     3841 ctccatccct cccgcggaga tcaaccggct cctcgcgatc ggggtgtcgg ggtgcctgtc
     3901 gaagtcgttg tcggcggagc ggctcgtcga cgaactcgag ctcatcgcca ccggcgagca
     3961 gcgcatcagt tcgttcgaag accttgcgaa ccccgtggtc gtcgccaacg ctccgggtgc
     4021 ccggtggggg ctcacctacc gggagagcga ggtgctcgcg ctgttgtccc tcggcatgcg
     4081 caacaaggag atcgccgcga cgctgtacgt cggcggtgag acggtcaaga gccacctcgc
     4141 gtcgatcttc cggaagatga aggtcgcatc gagggcggag gccatctcga ctgccgtccg
     4201 caccggcggg ttcgatcggg tcgcggaccc tcgctgagcg ttcagcgacc ggccggcgca
     4261 tcaaaccccc gaacggggca tcaggtcgtc gaaggcccgc cgaaatgggg ttcgtcgcca
     4321 cctcggggtc gaatcgaggc gccgcgtcga gcgcgtccga acgggggagg tgtgttcatc
     4381 ggtgatttcg ggaagggggt agccaatccc tcgatcgggc aatgagcccc ggtcgtcccg
     4441 ccgacacgag cacttcggag gccccgccat gaccgagccg atcaagatcg ccatcctcaa
     4501 cgactatgag ctggtggtcg cgggcatccg ccggatgctc gaaccgcacg cagggcgcgt
     4561 gaaggtggtc gagacggtcg tcggatcggc cgacgtcgat cacgaggtcg acatcgcgct
     4621 gtacgacacg ttcggtcgcg ccgacgtcgg cgtcgaccgc atctcggcgc tggtcgccaa
     4681 cccgcggatc ggccacgtcg tgatctacac gacgtcgatg ccggcggacg cgacccgaca
     4741 gctcctcgag ttgggtgtgt cggggtgcct gtcgaagtcg ttgagcgcca tcgacctcgt
     4801 tcgctcactc gaacgcgtgc accgcggcga ggtgacgatc gcgatgcgga gcagtggcgg
     4861 tccgtccgat ttctcgtcgc ccaacctgaa gctgagctac cgggagtcgg aggtcctggc
     4921 gctcttgagc caggggctcc gcaaccgtgc gatcgcgaag gcgttgtacg tcggcgagga
     4981 gacggtgaag agccatctca agagcatcta tcgcaagttg ggtgtgtcca gccgtggtga
     5041 agccatcgcg gttgcctttc gggagccgag cttcgccgaa ccgaaaccac tcgacgacga
     5101 ggcgatcagc ggctgaggtc gaggcgctcg gcgacctgac cttcgggctc ggtccaccgg
     5161 ctgacgaacg acgagccgac gatccgcccc ggttcagccc acgaccggag caagaatggg
     5221 agcgtgcccc gcaaccgtcc gcgccctgac gccgttcccg ccccgaccga tgacgaaatg
     5281 gcggcgctcg ccgcgctggg ccgcaacggc gagtgggagc tgcagggtcg tacgctcaag
     5341 ctgaccaacc tcgacaaggt gctgtttccc gcgggacgtg accggagcgg gatcgatcag
     5401 ccggcgttca ccaagcgcga tctcgtggcg cactacgcga gggtgggccc ggtgatggtg
     5461 ccgtacctcc gcggccgagc gctcaacatg aaccggttcc ccaacggggt cgaccaaccg
     5521 gggttctggc acaaggccgt gccgagccac gcaccggagt ggatgcagca gtggcagtac
     5581 gaacgggcgt cggagggcac gacgcggtgg tacttcgtgg tcgactcggt gccggcgctc
     5641 gcgtggatgg cgaacttcgg ggtcgtggag atgcacgcgt ggacctcacc gatcacggca
     5701 cccgaccggc cgagctacgc gctgatcgac ctcgaccccg gcacgacgac cacgttcgac
     5761 gatctcgtga cgctggcgct cctccaccgc accgcgttgg agcacctcgg ggtcacggcg
     5821 caaccgaagg tcaccggaca gcgcggcatc cagatctggg tgcccgtcga gcccgggccg
     5881 acgttcgagg acacccgcgt gtgggtggag aaggtgtcgc gtgcggtggg cgccatggtc
     5941 cccgatctcg tgagctgggc gtggaacacc aaggaacgca agggcctcgc ccgactcgac
     6001 tacacccaga acgcggtcaa caagacgctg gtcgcgccct acagcgtgcg cgccgcgatc
     6061 ggcgcgcccg tgtcgatgcc gatctcgtgg gacgagctcg acgaccccga cctccgcccc
     6121 gacggctggt cgatcgcgac ggcg
//
LOCUS       DAOSOA010000191         6132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12924_length_6132_cov_4.904723, whole genome shotgun sequence.
ACCESSION   DAOSOA010000191 DAOSOA010000000
VERSION     DAOSOA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12924_length_6132_cov_4.904723"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="aroC"
                     /locus_tag="PKY13_11515"
     CDS             complement(<1..720)
                     /gene="aroC"
                     /locus_tag="PKY13_11515"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT80610.1"
                     /translation="MSSSSGQVFRVSTYGESHGTAVGVVIDGCPPRLDLVEADIQADL
                     DRRRPGQSRLVTQRQESDRVEILSGLWEGVTTGTPIALEVRNADHRSSAYDHLADVYR
                     PSHADYTYDAKYGRRAIAGGGRASARETIGRVAAGAVARKLLAIGAGVEVLAWVSAVH
                     TIDATASIDPATVTLDEVEADPTRCPSPEHAAAMAGAIEQARRDGDSLGGVVTCVARG
                     VPAGLGAPVDTGDDAAEGVAVA"
     gene            756..2429
                     /locus_tag="PKY13_11520"
     CDS             756..2429
                     /locus_tag="PKY13_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80611.1"
                     /translation="MTWRKALWPHVVALGVILCVLVLAFGRNSVVRDGLVSADEGAVL
                     SQMEVRRTTGDWGMRNPLPAVDPDARWFGISGSDLDADRAYPYAKHVAYPAVAQPFFD
                     LGGARLVVGLSALGSLVTAALAALLARRVRRDLDVPTLWVVGIVSPIFFDSFWAIAHS
                     MGAAFATGAVLAAVVAIERRQARWIPLAALAVAAAALLRSEGMLFGVALGGGLLALAG
                     MRRDRLAAAAGVAAGAAASVAWWIDGRLHTAVVGATHPPFRISTTADQTWLSARWDGA
                     WASLLRPQLQATTTAGTIFLGVALAGALAALYVRAKPTEVRLIRGLAGIAGVAAIAAL
                     AFRVAPVPGLLVAFPILPIGLIVATRSTFESVTARIVGVTALLFSGAVVATQYAVGGS
                     MEWGGRFFHLAIPALVPVVLVSLDGARSRLDPTTRRITGAAIVAVSAVLVVFAVGSVA
                     RLQSQSGDLARAVHDLADGRVVVTNFESLGRFTWKDAVHGRFLSVPESDDLGTVAARL
                     ADEDIDEFVFAAPYRQETDRARLEGYEPVDGLTRTVGEWELSVMRRSTE"
     gene            2440..2967
                     /locus_tag="PKY13_11525"
     CDS             2440..2967
                     /locus_tag="PKY13_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80612.1"
                     /translation="MRAIIAVVVLCVLGALGTGCSDDSSAPPSDTTTVAPPSGPSSSV
                     GSASTEPETVDVCDALGTGLAEVGVDARPDRLTESGPTQCIFLGDTSTSFVGVLLTGN
                     TDSAAYEEALADPDLGDPLTVDDVGEMAARATRGTQDVFVAFGSGVLIELAQLGVIDG
                     DRLVEVGRYLMDLLT"
     gene            3019..5865
                     /gene="uvrA"
                     /locus_tag="PKY13_11530"
     CDS             3019..5865
                     /gene="uvrA"
                     /locus_tag="PKY13_11530"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT80613.1"
                     /translation="MADKIVIRGAKEHNLKNVSLELPRDKLIVFCGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGQMDKPDVEIIEGLSPAISIDQKSASRNPRSTVGTV
                     TEVYDYLRLLYARIGTPHDPETGEALVKQTPQQIVDKVLALPHGTRFQVLAPVVRGRK
                     GTYEKLFTDFAKQGFARVRVDGTVHELADITGDTPLELARYETHDIDVIVDRLVLRDD
                     LDRRLTESMETALVLGEGVAQIELLPKEADPEILTFSEKLSRPSDGKSFDELAPRNFS
                     FNSPYGYCPACQGLGMTFEVDPELVVPNAELSIAEGAIAPWAGGHAKYFHRLLESTCE
                     QYDVDPNVPWEQLSAAEQKLMLYGVNDKDRVEVKFRNRYGRTRSYSAKYEGVIPYISR
                     RHSEAETDSSREAAEGYMREVPCHTCKGARLNPYTLAVTVAGHNIFELCSLPIGEAAD
                     VIRTLDLDAREMQIAERVLKEIDTRLKFLSDVGLHYLNLHRGAATLSGGEAQRIRLAS
                     QIGSGLVGVLYVLDEPSIGLHQRDNHKLIETLQYLRDIGNTVVVVEHDEDTIAVADHV
                     VDIGPGAGEHGGEVVYSGNVAGLLKSRKSITGKYLSHRLSIPVPELRREPGDDWISIK
                     GARENNLRNVDVDIPLGCFVSVTGVSGSGKSTLINDILLKSLMQQIYGSKIPPGHHKK
                     ITGLEHLDKVINIDQSPIGRTPRSNPATYTGVFDHVRKLFAQTTEAKVRGYLPGRFSF
                     NVKGGRCEACAGDGTIKIEMHFLPDVYVPCDVCKGSRYNRDTLEILFKGLSISDVLNL
                     SCEEALAFFANQPPIARHMQTLVDVGLGYVRLGQAATTLSGGEAQRVKLASELAKRST
                     GHTIYLLDEPTTGLHFEDIRKLLGVLTRLVDQGNTVLVIEHNLDVIKTSDWVIDLGPE
                     GGNGGGTIVVEGPPEVVAKTPESHTGQFLAPLLS"
     gene            complement(5858..>6132)
                     /locus_tag="PKY13_11535"
     CDS             complement(5858..>6132)
                     /locus_tag="PKY13_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80614.1"
                     /translation="TKFSMPNKLFEYVMAGLAVVTSDLPDMAEIIDVHGVGATMAPTP
                     DGIAAALASFDRDRIDRCKAHAVVAAEALCWERERERLVELVAGVS"
BASE COUNT          948 a         2147 c         2056 g          981 t
ORIGIN      
        1 cgcgacggcg actccctcgg cggcgtcgtc acctgtgtcg accggtgcgc cgagcccggc
       61 gggaacgccc cgggcgacac aggtgacgac gccgccgagg gagtcgccgt cgcggcgggc
      121 ctgctcgatc gcgccggcca tggccgcggc gtgctcgggg gaggggcagc gggtcgggtc
      181 ggcctcgacc tcgtcgagcg tgaccgtcgc agggtcgatc gaggccgttg catcgatcgt
      241 gtggacggcg gagacccacg ccagcacctc gacgcctgcg ccgatcgcca acagcttgcg
      301 ggcaaccgcc ccggcggcga cacggccgat cgtctcacgg gccgaggccc gcccgccccc
      361 ggcgatggca cggcgcccgt acttggcgtc gtaggtgtag tcggcgtggg acggccggta
      421 gacgtcggcc aggtggtcgt aggcggacga ccggtgatcg gcgttgcgga cctcgagggc
      481 gatgggcgtt ccggtggtga cgccctccca caaccccgac aggatctcga cgcggtcgga
      541 ctcctgtcgc tgggtgacga ggcgactctg gcccgggcgg cgacgatcga ggtcggcctg
      601 gatgtcggcc tcgacgaggt ccaggcgggg cgggcagccg tcgatgacca caccgactgc
      661 cgtgccatgg gactcgccgt acgtggagac ccgaaagacc tgaccgctgc tcgatgacat
      721 gtaacaagta tggcgggagc gccgatggac tcctgatgac ttggagaaag gcgctctggc
      781 cgcacgtcgt ggcgctcggc gtgatcttgt gtgttctcgt gttggccttc gggcggaaca
      841 gcgtcgtccg cgacgggctc gtgagcgccg acgagggcgc cgtcctgtcg cagatggagg
      901 tccggcgcac caccggcgac tgggggatgc ggaacccgct cccggcggtc gatccggatg
      961 cccgatggtt cggcatcagc ggctccgatc tcgacgcgga tcgggcgtat ccctacgcga
     1021 aacacgtcgc ctatccggcc gtggcccagc cgttcttcga cctcggcggc gctcgcctcg
     1081 tcgtcggtct gtccgccctc ggctcgctcg tcacggccgc cctggccgcg ctgctcgctc
     1141 ggcgggtgcg ccgcgacctc gacgtgccga cgttgtgggt cgtcgggatc gtcagcccga
     1201 tcttcttcga ctcgttctgg gcgatcgccc actcgatggg agctgcgttc gcaaccggcg
     1261 ccgtgctcgc ggccgtggtc gcgatcgagc ggcggcaggc ccggtggatc ccgctggcag
     1321 cgctcgccgt cgcggcagcg gcattgctcc gcagcgaggg gatgctgttc ggggtggccc
     1381 tcggcggcgg actcctcgcg ttggccggaa tgcggcggga ccggctcgcc gctgccgccg
     1441 gggtcgccgc gggcgcggcg gcatccgtcg cgtggtggat cgacgggcga ttgcacactg
     1501 cggtcgtggg agcgacccat ccgcccttcc ggatctcgac gacggccgac cagacgtggc
     1561 tgtcggcccg atgggacggc gcatgggcgt cgctcctgcg tccccagctc caggcgacca
     1621 ccaccgccgg aacgatcttc ctgggggtgg cgctcgccgg agcgctggca gcgttgtacg
     1681 tgcgggccaa gcccaccgaa gttcgtctga tccgggggtt ggcgggaatc gccggcgtcg
     1741 ccgcgatcgc tgcgctggcg ttccgcgtcg cccccgtacc cggcctgttg gtcgcctttc
     1801 cgattctgcc gatcggtctg atcgtggcga cgaggtcgac cttcgagtcg gtgacggccc
     1861 gcatcgtcgg ggtgaccgcg ctcctcttct ccggtgccgt cgtggccacc cagtacgccg
     1921 tcgggggctc gatggagtgg ggcggcaggt tcttccacct ggcgatcccc gcgctcgtgc
     1981 ccgtcgtcct cgtgtcgctc gacggtgcgc gttcacgcct cgacccgacc acgcgacgca
     2041 tcaccggtgc agcgatcgtg gcggtgtcgg cggtgctggt cgtgttcgcc gtggggtcgg
     2101 tggcgcggct ccaatcccag agcggcgacc tcgcccgagc cgtccacgac cttgccgacg
     2161 gtcgcgtcgt cgtcacgaac ttcgaatcac tcggccggtt cacgtggaag gacgccgtgc
     2221 acggacgctt cctgagtgtt cccgagtccg acgatctcgg gaccgtcgcg gcgcgcctcg
     2281 ccgacgagga catcgacgag ttcgtcttcg cagcgccgta ccggcaggag accgaccggg
     2341 cccgactcga gggctacgag ccggtggacg gcctcacccg cacggtgggc gagtgggagt
     2401 tgtcggtgat gcggcggagc accgaatagc ctccaccggg tgagagcgat catcgccgtc
     2461 gtggtgctgt gtgtcctcgg tgccctcggt accggctgct ccgacgacag ctcggcgcct
     2521 ccgagtgaca ccacgactgt cgcaccgcca tcgggaccga gttcgtccgt cggctcggca
     2581 tcgaccgagc cggagaccgt cgacgtgtgc gacgccctgg gaacgggact ggcggaggtc
     2641 ggcgtcgacg cacgacccga tcgcctcacc gagagcggtc caacccagtg catcttcctc
     2701 ggcgatacgt ccacgtcgtt cgtcggggtg ttgctgaccg gaaacaccga cagcgcagcc
     2761 tacgaagagg cgctcgccga ccctgatctc ggcgaccctc tgacggtgga tgacgtcggc
     2821 gagatggcgg ctcgcgcaac ccgcggaact caggacgtgt tcgtcgcctt cggctcggga
     2881 gtgctcatcg agctggcgca gctgggggtg atcgacggcg accgtctggt cgaagtcggt
     2941 cggtacctca tggacctgtt gacctgaacc ccgccggacc gaacagacgt tcgtcgacgc
     3001 tgtgctaggc tcccgcgagt ggccgacaag atcgtcatcc gtggcgccaa agagcacaac
     3061 ctcaagaacg tctccctgga gcttccccgc gacaagctga tcgtcttctg cggtctgtcc
     3121 gggtcgggca agagctcgct ggcgttcgac accatctacg ccgagggcca gcgccgctac
     3181 gtcgagtcgc tgtcctcata cgcccgtcag ttcctgggtc agatggacaa acccgacgtc
     3241 gagatcatcg agggcctgtc cccggccatc tcgatcgacc agaagtcggc gtctcgcaac
     3301 cctcggtcga cggtcggcac cgtcaccgag gtctacgact acctccggct gctctacgcc
     3361 cgcatcggca cccctcacga tcccgagacg ggcgaggccc tcgtcaagca gacgccccag
     3421 cagatcgtcg acaaggtgct ggcccttccc cacggcaccc ggttccaggt gctcgcgccg
     3481 gtcgtgcggg gccgcaaggg cacctacgag aagctgttca ccgacttcgc caagcagggc
     3541 ttcgcccggg tgcgcgtcga cggtacggtg cacgagctgg ccgacatcac cggcgacacc
     3601 ccgctcgaac tggcccgcta cgagacccac gacatcgacg tgatcgtcga tcgtctggtg
     3661 ctccgcgacg acctcgaccg tcgcctcacc gagtccatgg agacggcgct ggtcctcggc
     3721 gagggcgtcg cccagatcga gctgctcccc aaggaagccg atccggagat cctcacgttc
     3781 tccgagaagt tgtcgcgccc gtcagacggc aagagcttcg acgagctggc cccccgcaac
     3841 ttctcgttca actcgcccta cgggtactgc ccggcctgcc agggcctcgg catgaccttc
     3901 gaggtcgatc ccgaactcgt cgtccccaac gctgagctgt cgatcgccga gggcgccatc
     3961 gccccgtggg ccggtggcca cgccaagtac ttccaccggc tgttggagtc gacgtgcgag
     4021 cagtacgacg tcgatccgaa cgtgccgtgg gaacagctca gcgcggccga gcagaagctc
     4081 atgttgtacg gggtcaacga caaggatcgt gtcgaggtca agttccgcaa ccgctacggg
     4141 cgcacccgca gctactcggc gaaatacgaa ggtgtcatcc cctacatctc ccgtcggcac
     4201 tccgaggccg agaccgactc cagtcgcgag gcggcggagg ggtacatgcg tgaggtgccg
     4261 tgccacacct gcaagggggc tcgactcaac ccgtacacgc tcgccgtgac ggtggccggc
     4321 cacaacatct tcgagctgtg cagcctgccg atcggcgagg cggccgacgt gatccgcacc
     4381 ctcgacctcg acgcccgcga gatgcagatc gccgaacggg tgctcaagga gatcgacacc
     4441 cggctcaagt tcctctccga cgtcgggctg cactatctca acctgcaccg cggcgccgcc
     4501 acactgtcgg gcggcgaggc ccagcggatc cggctcgcca gccagatcgg ctccgggctc
     4561 gtcggagtgc tctacgtctt ggacgagccg tcgatcgggc tgcaccagcg cgacaaccac
     4621 aagctcatcg agacgttgca gtacctccgc gacatcggca acacggtggt ggtcgtcgaa
     4681 cacgacgagg acaccatcgc cgtcgccgac catgtcgtcg acatcggccc cggtgccggt
     4741 gaacacggcg gcgaggtcgt gtactcgggc aacgtcgccg gcctcctcaa gagccggaaa
     4801 tcgatcacgg gcaagtacct gagccatcgg ctgtcgatcc ccgtccccga gctccgccgc
     4861 gaacctggcg acgactggat ctccatcaag ggcgcccgcg agaacaacct gcgcaacgtc
     4921 gacgtcgaca ttccgctcgg ctgcttcgtg tcggtcacgg gggtgtcggg ctcgggcaag
     4981 tcgaccctca tcaacgacat cttgctcaag tcactgatgc agcagatcta cggctcgaag
     5041 atcccgcccg gccaccacaa gaagatcacc gggctcgaac acctcgacaa ggtcatcaac
     5101 atcgaccagt cgccgatcgg ccgcaccccg cggtccaacc cggccacgta caccggtgtg
     5161 ttcgaccacg tgcgcaagct cttcgcccag acgacggaag cgaaggtgcg cggctacctg
     5221 cccgggcgct tcagcttcaa cgtcaagggc ggtcgctgcg aggcgtgcgc cggtgacggc
     5281 acgatcaaga tcgagatgca cttccttccc gacgtgtacg tgccgtgcga cgtgtgcaag
     5341 ggctcgcggt acaaccgcga caccctcgag atcctgttca aggggctctc gatttccgac
     5401 gtgctcaacc tgtcgtgcga ggaggcgttg gcgttcttcg ccaaccaacc gccgatcgcc
     5461 cgccacatgc agacgctcgt cgacgtgggg ttgggatacg tgcgactcgg ccaggcggcc
     5521 accacgctct cgggtggcga agcccagcgg gtcaagctgg cgtccgagct ggccaagcgg
     5581 tccaccggcc acaccatcta cctgctcgac gaacccacga ccggcttgca cttcgaggac
     5641 atccgcaagt tgctcggggt gctcacccga ctcgtcgacc agggcaacac cgtcctcgtc
     5701 atcgagcaca acctcgacgt catcaagacg tcggactggg tcatcgacct cgggcccgag
     5761 ggtggcaacg gcggtggcac gatcgtggtc gaggggccgc ccgaggtcgt ggccaagacg
     5821 cccgagagcc acaccgggca gttcctggcg ccattgctca gctgaccccg gcgaccagct
     5881 cgacgagccg ctcgcgctca cgctcccagc acagcgcctc cgcggcgacg accgcgtgcg
     5941 ccttgcaccg gtcgatccgg tcccggtcga acgacgccaa cgcggcggcg atgccgtcgg
     6001 gcgtcggcgc catcgtggcg ccgacgccgt gcacgtcgat gatctccgcc atgtcgggga
     6061 gatccgacgt gacgacggcg agaccggcca tcacgtactc gaagagcttg ttcggcatgg
     6121 agaacttcgt ct
//
LOCUS       DAOSOA010000192         6129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12937_length_6129_cov_5.548732, whole genome shotgun sequence.
ACCESSION   DAOSOA010000192 DAOSOA010000000
VERSION     DAOSOA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12937_length_6129_cov_5.548732"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PKY13_11540"
     CDS             complement(<1..1063)
                     /locus_tag="PKY13_11540"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80615.1"
                     /translation="MTRVPSIKASSMVPDRAENGSPLRLAVIGVVCVALFASLFVRLY
                     YLQVIDHKEFEVQSKRVHLRTKHEEGPRGRILDRNGNVLVDNKISVVVGIDKEIARGA
                     GLGDGDANDSDKELVKRKKVFQRLAGVLTAQGLPTKVTTIETLYTDVRYGPNDFIPIQ
                     DEGVGEPLKAYLAEHHTEFPGVVAKSRTVRVYPYGNLAAHLLGYVGQITEKELQLDDV
                     KSQGTTAKPAGKDAKPYALDDQIGKSGVERTYEKYLRGKPGDTVIQVDARGKSIGIVE
                     EPQLAQGDDVWLSIDINLQALTEQRLRDQIASRNPGAKDCKPACNAKDGAAVLLDPVN
                     GQVLAMASYPTFDPSELVNG"
     gene            complement(1060..1572)
                     /gene="mreD"
                     /locus_tag="PKY13_11545"
     CDS             complement(1060..1572)
                     /gene="mreD"
                     /locus_tag="PKY13_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOT80616.1"
                     /translation="MTPLHVAARWAAVIVVVFVLQVGLVSQMRVFGVIPDIMVVLAVC
                     AGLSGGGQRGAVVGFWIGLMFDLPRTEHPLGLSALAYCLVAFLAGMAQVVVLQSGRTI
                     SMALVAGGSVVGVLLFAVESQFFGGDALTNPHLLTILGVTALVGALLSRIGLRVAGFA
                     DGPETRSAAE"
     gene            complement(1569..2402)
                     /gene="mreC"
                     /locus_tag="PKY13_11550"
     CDS             complement(1569..2402)
                     /gene="mreC"
                     /locus_tag="PKY13_11550"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOT80617.1"
                     /translation="MPGSPRRRPRTTIIMMVLLSFTVITIGSKDVPVLGSVRGAVIDV
                     LGPVGRGFKSATKPVRSWWGGVNDYDRIAAENDQLRDEVERLKAKQAANSSAAAELAR
                     LKELEGLPFIADIDSKVALISTGAYSSFDDNTMTIDRGATSGFKVGMPVVDRSGLVGR
                     LESVFDDRSVIRLITDPDFAVYVKLASTGSYAAGHGTGPDRPFLVDSGVNLDQEVNKG
                     EGIFTSGIETGSFPRDLPIGTVTKVSASQSDLTQVLEVKLAADLERLSAVRVLLWEPP
                     T"
     gene            complement(2420..3463)
                     /locus_tag="PKY13_11555"
     CDS             complement(2420..3463)
                     /locus_tag="PKY13_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT80618.1"
                     /translation="MASRFSSFSSVLGRDMAIDLGTANTLVYVRGEGIVLREPSVVAV
                     NANTGQPLAVGSEAKRMIGRTPAHIRAVRPLRDGVINDFDICEKMLRYFIQKVHHRRF
                     ARPRMVICVPGGITGVEQRAVQEAAEYAGAAKPAYIIEEPMAAAIGSNLPVQEPRGNM
                     IVDIGGGTTEVAVISLGGVVASQSVRVAGDELDEAIIQFVKKEYSLALGERTAEEVKM
                     YLGSAAALDEEMQAEVRGRDLVSGLPKTIVTTTQEIREAIEEPVSAIVDAVKFTLDKT
                     PPELAADIMEQGIVLAGGGALLAGLNTRIANETGMPVRIADNPLDAVALGSGQCLESF
                     EALRGVLFSSSRD"
     gene            complement(3586..3993)
                     /gene="ndk"
                     /locus_tag="PKY13_11560"
     CDS             complement(3586..3993)
                     /gene="ndk"
                     /locus_tag="PKY13_11560"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOT80619.1"
                     /translation="MNRTLVICKPDAVERGLVGEILSRFERRQLKIVAAELRVIDRDL
                     ASQHYAEHEGKGFYEDLVSFIGRSPSLVFVVEGPDDTWKAVRSMMGATNPLDSAPGTI
                     RGDYGKDLGENLIHGSDSAESAAREIGIFFPGL"
     gene            complement(4052..>4444)
                     /locus_tag="PKY13_11565"
     CDS             complement(4052..>4444)
                     /locus_tag="PKY13_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80620.1"
                     /translation="TTTIPPTTTTTTTTTTIPPTTTTTTVAQSLSSALSDRSTNATSN
                     RWNAIVRVQLSATGGATTTGITITASFLEAGGSTTTRSCVTASNGRCDMSWNNRSDNR
                     AWVQVTITGVSGGAPWTGSANYIQLNKV"
     assembly_gap    4445..4544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4545..4905)
                     /locus_tag="PKY13_11570"
     CDS             complement(<4545..4905)
                     /locus_tag="PKY13_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80621.1"
                     /translation="MARAHRNRQRRARGEHGVTIVEYVLFFGCFVLVAVSAVGGLNSG
                     ARSYFQQSSSKIGQPVNRIQYDSNGARQQQASTTSITAPPATTTTVAPTTAPTTTTTT
                     TTTIPPTTTTTTTTTTIP"
     gene            complement(4999..>6129)
                     /locus_tag="PKY13_11575"
     CDS             complement(4999..>6129)
                     /locus_tag="PKY13_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOT80622.1"
                     /translation="KGSVCRMVEALVGAAGLRVGTYLSPEGTPNERIRIDGRPIDDVS
                     LADAIGSVRGVADVLDLQLTAFEAITLAAIVAFGDAPVDVAVIEVGLLGRFDATNIID
                     GDVSVVTTIGGDHTDFAAGWRDRIASEKAGILKATSQAVLGEIDDDVIAHFTAEGPES
                     LVRLDHDFECLDDRVALGGRRAEVTTSRGSRFEVFIPLHGSHQAQNAAVAIEATESVL
                     HAQLDPDVVDAAFETLRIHGRIEMVSADPVVVVDGAHNPDAAGALGRTLSESFVIAGR
                     RVAVIGMLQGRDPLAFLSALNDEYPIDLVIGCSLPAPRGAPGSAVAAAAAQLGIPAVQ
                     SDDIASGVSRALDQSDEADLIVVTGSFRVVDDATAVVRRRAR"
BASE COUNT          966 a         1975 c         2087 g         1001 t
ORIGIN      
        1 tgccgttcac cagctccgac gggtcgaacg tcgggtacga cgccatcgcc agcacctggc
       61 cgttcacagg atcgagcagc accgcggcgc cgtccttggc gttgcacgct ggcttgcagt
      121 ccttggcccc ggggttccga gacgcgatct ggtcgcgcag tcgttgttcg gtcagcgcct
      181 ggaggttgat gtcgatcgac aaccagacgt cgtcgccctg ggcgagctgg ggttcctcga
      241 cgatgccgat gctcttgccc cgggcgtcga cctggatcac cgtgtcgcct ggcttgcctc
      301 gcaggtactt ctcgtaggtg cgctcgacac cgctcttgcc gatctggtcg tcgagcgcat
      361 acggcttggc atccttgccg gccggcttgg ccgtggtgcc ctggctcttg acgtcgtcga
      421 gctggagctc cttctcggtg atctgtccga cgtacccgag gaggtgcgct gcgaggttgc
      481 cgtaggggta cacccgcacc gtgcgcgact tggccaccac accggggaac tcggtgtggt
      541 gttcggcgag gtacgccttg agcggctccc cgacgccctc gtcctggatg gggatgaagt
      601 cgttcgggcc gtaccgcacg tcggtgtaga gcgtctcgat cgtcgtgacc ttcgtgggca
      661 ggccctgggc ggtgagcaca ccggccaaac gctggaacac cttcttgcgc ttgaccagct
      721 ccttgtcgct gtcgtttgcg tcgccgtcac cgagcccggc gccacgagcg atctccttgt
      781 cgatgcccac caccaccgag atcttgttgt cgaccagcac gttgccgttg cggtcgagga
      841 tgcggccccg cggcccctcc tcgtgcttgg tacggaggtg cacccgcttg gactgcacct
      901 cgaactcttt gtggtcgatc acctggaggt agtagaggcg gacgaacagg gacgcgaaga
      961 gcgccacaca gacgacgccg atgaccgcca ggcggagcgg cgaaccgttc tcggcccggt
     1021 caggaaccat ggaggacgcc ttgatcgagg gcacgcgcgt cactccgcgg cgctacgggt
     1081 ctcggggcca tcggcaaagc ctgccactcg gagcccgatt cgtgacagca gggcgccgac
     1141 gagcgccgtc acgcccagga tcgtcaggag gtgggggttg gtgagggcat ccccgccgaa
     1201 gaactgcgac tcgacggcga acagcagcac gccgaccacg ctgccaccgg ccacgagcgc
     1261 catcgagatc gtccgacccg actgcagcac gacgacctgg gccataccgg ccaggaacgc
     1321 cacgaggcag tacgcgagcg ccgacaggcc caggggatgt tcggtgcgcg gcaggtcgaa
     1381 catcaacccg atccagaacc cgaccaccgc gccgcgctgt cccccaccgc tcagtccggc
     1441 gcacaccgcg agcacgacca tgatgtcggg gatcacaccg aacacgcgca tctggctcac
     1501 gagcccgacc tgcagtacga acaccacaac gatgaccgcc gcccaccggg cggcgacgtg
     1561 cagcggtgtc atgtgggcgg ctcccacagc agcacccgga cggcgctgag tcgttcgagg
     1621 tcggccgcca acttcacctc gagcacctgg gtgaggtcgg actggctcgc cgacaccttg
     1681 gtgaccgtgc cgatcggaag gtcgcggggg aacgagccgg tttcgatgcc gctcgtgaag
     1741 atgccctcgc ccttgttgac ctcctggtcg aggttcacgc ccgagtcgac gaggaacggt
     1801 cggtcgggac cggtgccgtg gccggcagcg taggaaccgg tgctcgcgag cttcacgtac
     1861 accgcgaaat cggggtcggt gatgagtcgg atcacggaac ggtcgtcgaa caccgattcg
     1921 agccggccga cgaggccgga ccggtcgacg acgggcatgc cgaccttgaa gcccgaggtc
     1981 gctccccgat cgatggtcat cgtgttgtcg tcgaagctgg agtaggcacc ggtcgagatg
     2041 agggcgacct tcgagtcgat gtcggcgatg aacggcaggc cttcgagttc cttcagacgg
     2101 gcgagttcgg cagcggccga cgagttggcc gcctgcttgg ccttgagtcg ctcgacctcg
     2161 tcgcgcagct ggtcgttctc ggcggcgatg cggtcgtagt cgttgacgcc accccaccag
     2221 ctccggaccg gcttggtcgc cgacttgaag ccccggccca ccggaccgag gacatcgatc
     2281 accgcgccgc gaaccgagcc gagcaccggc acgtccttcg atccgatcgt gatcaccgtg
     2341 aacgacaaca acaccatcat gatgatcgtg gtgcgcggtc gacggcgggg agagcctggc
     2401 atcggtgttc ggccctgcgt cagtcgcggc tggacgagaa gagcacgccc ctcaacgcct
     2461 cgaacgactc gaggcactgc cccgagccca acgccacggc atcgaggggg ttgtccgcga
     2521 tgcggaccgg cataccggtc tcgttggcga tgcgagtgtt gagaccggca aggagcgcgc
     2581 caccaccggc aaggacgatg ccctgctcca tgatgtcggc ggccaactcc ggcggcgtct
     2641 tgtcgagcgt gaacttcacg gcgtccacga ttgcagacac cggctcctcg atggcttcgc
     2701 gaatctcctg cgtcgtggtc acgatggtct tgggaagccc cgagaccagg tcgcggcctc
     2761 ggacctccgc ttgcatctcc tcgtcgagcg cagcggccga accgaggtac atcttgacct
     2821 cctcggcggt gcgctcaccg agcgcaaggc tgtactcctt cttcacgaac tggatgatcg
     2881 cctcgtcgag ctcgtcgccc gcgacacgca ccgactgact cgcgacgacg ccgccgagcg
     2941 agatgacggc cacctcggtg gtgccaccgc cgatgtcgac gatcatgttg ccgcggggct
     3001 cttgcaccgg gaggttggag ccgatggccg cggccatggg ctcctcgatg atgtacgcgg
     3061 gcttggccgc gccggcgtat tccgcagctt cctgcacggc tcgttgctcg acgccggtga
     3121 tgccaccggg cacgcagatc accatgcggg gccgtgcgaa tcgacggtga tgcaccttct
     3181 ggatgaagta gcggagcatc ttctcgcaga tgtcgaagtc gttgatgacc ccgtcgcgaa
     3241 gcggccgaac ggcgcggatg tgcgccgggg tacggccgat catgcgcttg gcttcgctcc
     3301 cgaccgcgag tggctggccg gtgttggcgt tgaccgccac caccgagggc tcacgcagca
     3361 cgatgccctc gccccggacg tagaccaacg tgttggccgt gccgagatcg atggccatgt
     3421 cccgcccgag aacgctggag aacgaggaaa accgggatgc catggctacg cagctcctcc
     3481 agggaagtcg cagcgaaggt atccgcgact cgggactcgg gacatgctag ctggttgccg
     3541 gtcccgccct ccagcgcctt gggcgccgac gccgacggcg acggctcaga gacccgggaa
     3601 gaagatgccg atctcccggg ccgccgactc ggcggagtcg gagccgtgga tcaggttctc
     3661 gccgaggtcc ttgccgtagt caccacggat ggtgccgggg gccgagtcga gcgggttggt
     3721 cgcgcccatc atcgaacgca cggccttcca cgtgtcgtcg ggaccctcga cgacgaacac
     3781 gagcgacggg gagcggccga tgaacgagac gaggtcttcg tagaagccct tgccctcgtg
     3841 ctcggcgtag tgctgggagg cgagatcgcg gtcgatcact cgcagctcgg cggcgacgat
     3901 cttgagctgg cggcgctcga agcgggagag gatctctccg accaggccac gttcgacggc
     3961 gtcgggcttg cagatgacga gagtgcgatt catgcggcga aggatacggg gctcaccggg
     4021 cgccgacgaa tcggcgagcc ccgccgccac ctcagacctt gttgagctgg atgtagttcg
     4081 ccgacccggt ccacggggcg ccaccgctga cgccggtgat cgtcacctgt acccaggcac
     4141 ggttgtccga tcggttgttc cagctcatgt cgcagcgacc gttgctggcc gtgacacacg
     4201 acctggtcgt ggtcgatcca cccgcctcca ggaacgaggc ggtgatcgtg atacccgtgg
     4261 tggttgcgcc accagtcgcc gagagttgca ctcgcacgat ggcgttccag cggttgctgg
     4321 tcgcattcgt cgaccggtcc gacagggccg agctcaggct ctgtgcaacc gtcgtcgtgg
     4381 tggtggtcgg cgggatggtg gtggtcgtcg tggtcgtcgt tgtggtcggc gggatggtgg
     4441 tcgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcggga tggtggtggt
     4561 cgtcgtggtc gtcgttgtgg tcggcgggat ggtggtcgta gtggtcgtgg tggtcgtcgg
     4621 agcggtggtc ggcgcaaccg tcgttgtggt cgcgggcggg gcggtgatgc tggtcgtgct
     4681 cgcctgttgc tggcgggcgc cgttggagtc gtactggatc cggttgaccg gctggccgat
     4741 cttgctcgac gactgctgga agtacgagcg ggcaccgctg ttcagcccgc cgacggcgga
     4801 aacggcgacc aggacgaagc atccgaagaa gaggacgtac tccacgatcg tgacgccatg
     4861 ctcgccgcgg gctcgccgct gccggttcct gtgtgctcgc gccatcgagg gtctcctgag
     4921 ttggacccac atgtcttcgg cgaatctgcc cgcgaactga aggacaagtt tcgctgaccg
     4981 cggccgtggc gaccccggct atcgagcgcg gcgacgaacg acggcagttg cgtcgtcgac
     5041 gacccggaac gacccggtga cgacgatcag gtcggcctcg tcgctctggt cgagtgcacg
     5101 gctgacgcca ctcgcgatgt cgtcggactg cacggcggga atgccgagct gggccgccgc
     5161 cgcagcgacc gccgaacccg gtgccccgcg gggagccggc agcgaacatc cgatgacgag
     5221 gtcgatcggg tactcgtcgt tgagcgcgga cagaaacgcc agcggatcac gtccctggag
     5281 catgccgatc acggcgaccc gtcggccggc gatcacaaac gactcggaca gcgtgcgacc
     5341 gagggccccc gcagcatcgg ggttgtgggc cccgtcgacc accacgaccg gatcggcgga
     5401 caccatctcg atccgaccgt gaatccggag cgtctcgaag gccgcgtcga cgacgtcggg
     5461 atcgagctgg gcgtggagca ccgattcggt cgcctcgatg gcgacggcag cattctgcgc
     5521 ctggtgcgag ccgtggaggg gaatgaagac ctcgaaccgt gagccccgcg acgtggtgac
     5581 ctcggcacga cgcccaccga gtgcgacgcg gtcgtcgagg cactcgaagt cgtggtcgag
     5641 acggacgagc gattcgggac cctccgcggt gaagtgcgcg atgacgtcgt cgtcgatctc
     5701 gccgaggacc gcttggctcg tcgccttgag aatccccgcc ttctccgagg cgatccggtc
     5761 gcgccagccg gccgcgaagt cggtgtggtc accgccgatc gtcgtcacga ccgagacatc
     5821 gccgtcgatg atgttggtcg cgtcgaaccg gccgagcagg ccgacctcga tgacggccac
     5881 gtcgacgggc gcatcaccga acgcgacgat cgctgcgagc gtgatggcct cgaacgcggt
     5941 cagctggagg tcgagcacgt cggcaacacc gcgcaccgac ccgatcgcgt cggcgaggga
     6001 cacgtcgtcg atggggcgac cgtcgatgcg gatccgttcg ttcggggtcc cctcggggct
     6061 gaggtacgtg cccacccgga ggccggcggc gccgacgagg gcctcgacca tgcgacacac
     6121 cgagccctt
//
LOCUS       DAOSOA010000193         6114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_12981_length_6114_cov_7.141277, whole genome shotgun sequence.
ACCESSION   DAOSOA010000193 DAOSOA010000000
VERSION     DAOSOA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12981_length_6114_cov_7.141277"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1038
                     /locus_tag="PKY13_11580"
     CDS             130..1038
                     /locus_tag="PKY13_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOT80623.1"
                     /translation="MAGALDRLKERVDTFQRGNGFAGFVFGVVKKAGDDKGSHLAALI
                     TYYGFFSLFPLLLVAFTILGFVLQGNDSLRNDVASTLQERLPLPGVTAESLNGSGLAL
                     VLGIVLTLWSGLGATQVAQDALNTIWDVPRKKQPNFFKKRLVGLGTLGVIGVGLILAT
                     AASSLAGFLGPAAGVGGAAASVLLNTLVIAGLFKLMVERDLSWTEIRLGAFVGGIGWT
                     ALQLIGSWYTQRLVDNADKTYGTFAVVIGLLSWIYLQSQVFVYAAEVASVSSRRLWPR
                     ALVAERPTEADRVVERALVERSKPAS"
     gene            complement(1043..2071)
                     /locus_tag="PKY13_11585"
     CDS             complement(1043..2071)
                     /locus_tag="PKY13_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT80624.1"
                     /translation="MTTTGSVDTATAALRRAIYYLDRSLAPAPKVRAFRTALSVVEGL
                     GDDELDRHLAARSITELDGIGPSTGAVITEAFADGSKPGYLSKLESSTRLEVGEGAEL
                     RTRLRGDCHLHSTWSDGGASIEDMARTAIDLGHEYIVLTDHSPRLTIAHGLSQERLDE
                     QRVEVERLNELLSPFRILRGVEVDILEDGTLDHSDEVLAGLDIVVASVHSKLKMPADQ
                     MTRRMVMAVANPNVDILGHCTGRQIVGTPRAPSQFDAEIVFAACARFGTAVEINCRPE
                     RQDPPDELLELALDWDCVVSIDTDAHAPGQMEWQISGCDKAVRHGIDDAKIVNTMSAD
                     DLLAWAAN"
     gene            2137..2817
                     /locus_tag="PKY13_11590"
     CDS             2137..2817
                     /locus_tag="PKY13_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT80625.1"
                     /translation="MLPASRRRSTLFLVLAIALLATACLPSKTAAGRPGPVRKVALLG
                     DSLTFGLFGTTPGVADPLRERLGASGLSVTIDGGPGDTLQTPWPGHANWADLLQARID
                     QDDPDVVIIQSILFPGAENPANHDSYLRAAEKLIDIGQSRGAHVYFVQHHRPNNSTEF
                     NAAEIAERLQGIAAEGRGIGVIPMNWWIARCASPYAFDGWHLSGNGVRCWADAANAAI
                     NQLRNELG"
     gene            complement(2851..3621)
                     /locus_tag="PKY13_11595"
     CDS             complement(2851..3621)
                     /locus_tag="PKY13_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT80626.1"
                     /translation="MSNRPKPKSKIQYGTPAPGAPGSPKRPGEGGPMGAKIAIGAAIA
                     VVLIAVIVFAVKDGGGDNQNKVAFQPATVTGAALPNLPDDGSADPAVGQQIPTVVGKS
                     FDGSKVEIKSGEPQLIAFIAHWCPHCQAEVPLIAEWQADDSLGDIKITGVSTAADETR
                     GNFPPASWLEKESWKNETLVDDPTSKVLNAFGATSFPTIVAVKADGTVALRLTGELTL
                     DQVKQLVDAAEGKEVAPVDSTTDTTPLTSPAPTTTVAP"
     gene            complement(3625..>3819)
                     /locus_tag="PKY13_11600"
     CDS             complement(3625..>3819)
                     /locus_tag="PKY13_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80627.1"
                     /translation="HILIDTGVVSESSSCDPLNPCTTRWLGFEHGWSTIQVGAFCCFL
                     FIIGLGLHALAKPSESPAEA"
     assembly_gap    3820..3919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4101..5177
                     /gene="ychF"
                     /locus_tag="PKY13_11605"
     CDS             4101..5177
                     /gene="ychF"
                     /locus_tag="PKY13_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOT80628.1"
                     /translation="MERFGFVGLPNAGKSSLYNALAGGGALAAPYAFATVDPNVGMAK
                     VPDPRVEALAKMSNTKTLVHAAVQFVDIAGLVKGAAQGEGLGNRFLAHIREVDAIVFV
                     LRAFEDDDVPGSHDPVEQLETLEVELIFADLETLEKQIDKRRKAAKGDKSLVDEVAAM
                     QQAIEVLQRGVALYRSDLDAETRDLLKPYFLLTNKPAMAVVNVDESQLEDVDAVIAPV
                     VETMGGAAEVVGACVQLEAEAVLLDEEERTEMLEALGLGDGALARFLRSAYQMLGLRT
                     FLTTGEKETRAWTFRAGAKAPEAAGVIHTDFQRGFIRAETIQWDELLECGSWAKAREV
                     GKARVEGKDYVVQDGDVMDFRFNV"
     gene            5190..5516
                     /locus_tag="PKY13_11610"
     CDS             5190..5516
                     /locus_tag="PKY13_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOT80629.1"
                     /translation="MGWLVRDGEVLASLEVATNAGRRAKGLLGRDGIDGALLIPKTRS
                     VHSIGMRFDLDVAFLDGDQRVVKVVRLKRGRMTLPVWSARSVVEAEAGSFRDWGLRCG
                     DELEVR"
     gene            5513..>6114
                     /gene="rsmI"
                     /locus_tag="PKY13_11615"
     CDS             5513..>6114
                     /gene="rsmI"
                     /locus_tag="PKY13_11615"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT80630.1"
                     /translation="MTAPILVVGTPIGNLGDVSHRAVEAMTAATVIACEDTRRTGSLL
                     RHLGIERRRLVVLNDHTERDVVDRLVADAIADERVVLVSDAGMPAISDPGYLLVRAAI
                     EADVRLEVVPGPSALLAALVLSGLTTDRFVFDGFLPRKGGERSVRLAEVAAQSRTTIL
                     YEAPHRLERTLVDLEKACGGDRPVAVARELTKLYEEVWRGT"
BASE COUNT          879 a         1963 c         2161 g         1011 t
ORIGIN      
        1 ggcttgcgcc cgtggttggc cttcttgcgg cgaacgttcg ccttcttctt gctggttcgc
       61 ttcgacatag gggaacaacc ctagcggtga gaggctcacc tggggcaaat gcgggcatag
      121 ggtgcgccga tggcaggagc actggatcga ctgaaggaac gggtggacac gttccaacgc
      181 gggaacgggt tcgccgggtt cgtgttcggt gtcgtcaaga aggcggggga cgacaagggc
      241 tcgcatctgg cggccctgat cacctactac gggttcttct cgctgttccc gctcctgctc
      301 gtcgccttca cgatcctcgg cttcgtactg cagggcaacg actcgttgcg caacgacgtg
      361 gcgtcgaccc tgcaggagcg tctgccgctc cccggggtca cggccgagtc gctgaacggg
      421 agcggcctcg ctctggttct cggcatcgtc ctgacgttgt ggtcgggtct cggggccact
      481 caggtggcgc aggacgcgtt gaacacgatc tgggacgtgc cccgcaagaa acagccgaac
      541 ttcttcaaga aacggctcgt tgggctcggc acgctcgggg tgatcggcgt cgggctcatc
      601 ttggccaccg cggcgtcctc gctcgccggc ttcctgggac cagcggccgg cgtgggtggc
      661 gcagctgcat ccgtgctcct caacacgttg gtgatcgccg gattgttcaa gctgatggtc
      721 gagcgtgacc tgtcgtggac ggagatccgg ctcggcgcgt tcgtcggggg catcggctgg
      781 acggcgctgc agctgatcgg cagctggtac acccagcggc tggtggacaa tgccgacaag
      841 acctacggca ccttcgccgt ggtcatcggc ctgctctcgt ggatctacct gcagtcgcag
      901 gtgttcgtgt acgccgccga ggtcgcgtcg gtgtcctccc gcaggctctg gccgcgggcg
      961 ctggtcgcgg agcggccgac cgaggccgac cgcgtggtcg aacgcgccct cgtcgaacgc
     1021 tccaagccgg cgagctgacg tctcagttcg ccgcccaggc gagcaggtcg tcggcgctca
     1081 tcgtgttcac gatcttggcg tcgtcgatcc cgtggcggac ggccttgtcg cagcccgaga
     1141 tctgccattc catctggccg ggggcgtgag cgtcggtgtc gatggacacc acgcagtccc
     1201 agtcgagcgc gagctcgagc agttcgtcgg gcggatcctg gcgttccggg cggcagttga
     1261 tctcgacggc ggtcccgaac cgggcgcagg ccgcgaagac gatctcggcg tcgaactgtg
     1321 acggcgcccg cggcgtgccg acgatctgtc ggccggtgca gtggccgagg atgtcgacgt
     1381 tgggattggc gacggccatg accatgcgcc gggtcatctg gtcggcgggc atcttcagct
     1441 tggagtgcac ggaggcgacg acgatgtcga ggcctgccag cacctcgtcg gagtggtcga
     1501 gggtgccgtc ctcgaggatg tcgacctcga caccacggag gatacggaac gggctcagca
     1561 gctcgttcag gcgctcgacc tcgacgcgtt gctcgtcgag ccgctcctgg gacaggccgt
     1621 gggcgatggt caggcgaggc gaatggtcgg tgaggacgat gtactcgtgt ccgagatcga
     1681 tggccgtgcg ggccatgtcc tcgatcgatg caccaccgtc ggaccacgtg ctgtggaggt
     1741 ggcagtcgcc gcggagtcgg gtccggagct cggcgccctc gccgacctcg aggcgggtcg
     1801 acgactcgag cttcgagagg taccccggtt tcgaaccgtc ggcgaacgct tcggtgatga
     1861 cggcgccggt gctcggcccg atgccgtcga gttcggtgat gctgcgggcc gcgagatggc
     1921 ggtcgagctc gtcgtcgccg aggccctcga ccacggacag ggccgtgcgg aacgcccgca
     1981 ccttcggagc gggagcgagc gatcggtcga ggtagtagat cgcccgacgc agcgcagcgg
     2041 tcgcggtgtc cacggatccg gtcgttgtca tcgacggtca tcgtagagcg gccgccgcgt
     2101 tgccgacggt cgtggtccgc gttctaccct gacccgatgc tccccgcttc ccgccgccgc
     2161 tcgaccctgt tcctcgtcct cgcgatcgcc ctgctggcga ccgcgtgtct gccctccaag
     2221 acggccgccg gccgccccgg tccggtgcgc aaggtcgcac tgctcggcga ctccctcacc
     2281 ttcggcctct tcggcaccac cccgggggtg gccgacccgt tgcgtgagcg cctcggagcg
     2341 tcgggcctct cggtcaccat cgacggcgga ccgggcgaca cgctgcagac cccgtggccc
     2401 ggccacgcca actgggcgga cctcctgcag gcccggatcg accaggacga ccctgatgtg
     2461 gtcatcatcc agtcgatcct gttccccggc gccgagaacc ccgccaacca cgacagctac
     2521 ctgcgcgccg ccgagaagct gatcgacatc ggccagtcgc gcggtgccca cgtgtacttc
     2581 gtgcagcacc accggccgaa caactcgacg gagttcaacg ccgccgagat cgccgagcgg
     2641 ttgcagggca tcgccgccga gggccggggg atcggcgtca tcccgatgaa ctggtggatc
     2701 gcacgctgcg cgtcgccgta cgcgttcgat gggtggcacc tgagcggcaa cggtgtccgc
     2761 tgctgggcgg acgcggcgaa cgccgcgatc aaccagctgc gcaacgagct gggctgaccg
     2821 cggcgttcgc ggcggagggt cgacgttccc ttacggagcg acggtcgtcg tcggcgcggg
     2881 ggaggtgagc ggcgtcgtgt ccgtggtgct gtcgacgggg gcgacttcct tgccttcagc
     2941 ggcatccacg agttgcttga cctgatcgag agtcagctcg ccggtcagcc ggagggcgac
     3001 cgtgccgtcg gccttgactg cgacgatggt cgggaagctg gtcgctccga acgcgttgag
     3061 caccttgctc gtcggatcgt cgaccagcgt ctcgttcttc caggactcct tctcgagcca
     3121 cgacgccggc gggaagttgc cgcgggtttc gtcggccgcc gtcgacacac cggtgatctt
     3181 gatgtcgccg aggctgtcat cggcctgcca ctcggcgatc aacggaacct cggcctggca
     3241 gtgggggcac cagtgggcga tgaacgcgat cagctgcggc tcgccgctct tgatctcgac
     3301 cttcgagcca tcgaacgact tgcccacgac cgtcgggatc tgctggccca cggccgggtc
     3361 ggccgaaccg tcgtcgggca ggttcggcag cgccgcgccg gtcaccgtgg cgggctggaa
     3421 cgcgaccttg ttctggttgt cgccgccgcc gtccttgacc gcgaagacga tcacggcgat
     3481 gagcaccacg gcgatcgccg cgccgatggc gatcttggcg cccatcgggc cgccctcacc
     3541 cggccgcttg ggggagccgg gcgcgcccgg cgccggcgtg ccgtactgga tcttgctctt
     3601 gggcttgggt cggttggaca tgggtcacgc ctccgcaggt gactcggacg gcttggccaa
     3661 cgcgtggagg ccgagcccga tgatgaacag gaaacaacag aacgcaccga cctggatggt
     3721 cgaccagccg tgctcaaagc ccagccagcg cgtggtgcag gggttgagcg ggtcgcacga
     3781 cgaactctcc gacacgacgc ccgtgtcgat gaggatgtgn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnng atcaggtcgt ccatgggttg cccttcacga gagtgcccga
     3961 gtgggcgcgg cgggatgcgt cgtcgtaccg gtcggcagtt tggcgcggtg actgaggcgg
     4021 gacatggaag gtcctgtgag gattcgaagg tgggacgggg ttcggagccc acccatggcc
     4081 gcaggtagcg taggcgaacc atggaacgat tcgggtttgt cggactcccc aatgcgggga
     4141 agtcatcgct gtacaacgca ctcgcgggcg gtggtgccct cgcggcgccg tacgcgttcg
     4201 cgaccgtcga tcccaacgtc ggcatggcga aagtgcccga tccgcgggtc gaggcgctcg
     4261 ccaagatgtc gaacaccaag acgctcgtgc acgccgccgt gcagttcgtc gatatcgccg
     4321 gcctggtgaa gggcgccgcc cagggcgagg gactcggcaa ccggttcctg gcccacatcc
     4381 gcgaggtcga cgccatcgtg ttcgtgctgc gggccttcga ggacgacgat gttcccgggt
     4441 cccacgatcc cgtggagcag ctcgagaccc tcgaggtgga gctgatcttc gccgacctcg
     4501 agacgctcga gaagcagatc gacaaacgtc gcaaggccgc caagggcgac aagtcgctcg
     4561 tcgacgaggt ggccgcgatg cagcaggcga tcgaggtgct ccaacgcggg gtcgcgctct
     4621 accgcagcga cctcgacgcc gagacccgcg accttctcaa gccgtacttc ctcctgacga
     4681 acaagccggc gatggccgtc gtcaacgtcg acgagtcgca gctcgaggac gtcgacgccg
     4741 tgatcgcccc ggtggtcgag accatggggg gtgccgccga agtcgtcggt gcgtgcgtac
     4801 agctcgaggc cgaggccgtg ctcctcgacg aggaggagcg caccgagatg ttggaggcgc
     4861 tgggcctcgg cgacggggcg ctcgcccgtt tcctccggag cgcctaccag atgctcggtc
     4921 tgcggacctt cctgaccacc ggtgagaagg agacccgtgc ctggacgttc cgggccgggg
     4981 ccaaggcgcc cgaggccgcc ggcgtgatcc acaccgactt ccagcgtggc ttcatccggg
     5041 ccgagacgat ccagtgggac gaactcctcg agtgcggttc gtgggcgaag gcccgcgagg
     5101 tcggcaaggc ccgcgtcgag ggcaaggact acgtggtgca ggatggcgac gtcatggact
     5161 tccgtttcaa cgtgtgagga tgggtggccg tgggttggct ggtgcgggat ggtgaggtgc
     5221 tggcgagcct cgaggtcgcg accaacgcag ggcgtcgcgc caaggggctc ctcggtcgcg
     5281 acggcatcga cggcgccctg ctcatcccga agacccgctc cgtgcacagc atcggcatgc
     5341 gcttcgatct cgacgtcgcg ttcctcgacg gcgaccagcg ggtggtgaag gtggtgcgtc
     5401 tcaaacgcgg ccggatgaca ctcccggtgt ggtcggcccg ttcggtcgtg gaggccgagg
     5461 ccggctcgtt ccgcgactgg ggcctgcgct gcggcgacga actcgaggtc cggtgacggc
     5521 ccccatcctg gtggtgggca ccccgatcgg gaacctcggc gatgtcagcc accgcgccgt
     5581 cgaggcgatg acggcggcca cggtgatcgc ctgtgaggac acgcggcgga ccgggtcgct
     5641 gctccgtcac ctcggcatcg aacgtcggcg tctcgtcgtg ctcaatgatc acaccgagcg
     5701 tgatgtcgtc gaccgtctcg tcgccgacgc gatcgccgac gagcgcgtcg tgctcgtgag
     5761 tgacgccggc atgccggcga tcagcgaccc ggggtacctc ctcgtgagag cggcgatcga
     5821 ggcggacgta cggctcgagg tcgtgcccgg tccctcggcg ctgctcgctg cgctggtgct
     5881 cagcggactc acgaccgacc ggttcgtgtt cgacggcttc ctgccccgca agggcggcga
     5941 gcggtcggtg cgcctggctg aggtggcggc gcagtcccgc acgacgatcc tctacgaggc
     6001 gccgcaccgg ctggaacgaa cgctcgtcga tctggagaag gcgtgcggtg gcgaccggcc
     6061 ggtcgcggtg gcccgggagc tgaccaagct ctacgaggag gtgtggcggg gcac
//
LOCUS       DAOSOA010000194         6108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13003_length_6108_cov_5.558896, whole genome shotgun sequence.
ACCESSION   DAOSOA010000194 DAOSOA010000000
VERSION     DAOSOA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13003_length_6108_cov_5.558896"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PKY13_11620"
     CDS             <1..351
                     /locus_tag="PKY13_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOT80631.1"
                     /translation="ARMVESVAAAQRAGVETVRAGATSREVDQACRAVIDEYGWAERF
                     THGTGHGVGLDIHEAPRVSAVSDATLEPGFVVTVEPGVYIAPHGGVRIEDTVVVTETG
                     CTVLTASPKTLLVA"
     gene            404..973
                     /gene="efp"
                     /locus_tag="PKY13_11625"
     CDS             404..973
                     /gene="efp"
                     /locus_tag="PKY13_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT80632.1"
                     /translation="MAQISTADFKNGITLDLPEGLVQIVEFQHVKPGKGGAFVRTTLK
                     NVRNGAVVDRTFRAGEKMERAFIEKKDMQFLYNDGADYVFMDTTSYEQLSVPPASLGD
                     SKNYLLENMTAAVQMFGDEIIGVELPAAVDLPIRETEPGVQGDRVSGARKPATLITGL
                     VVQVPLFVGPDDIIRVDTRTGEYITRANG"
     gene            970..1383
                     /gene="nusB"
                     /locus_tag="PKY13_11630"
     CDS             970..1383
                     /gene="nusB"
                     /locus_tag="PKY13_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT80633.1"
                     /translation="MTDISSAEVGRHEARERAVQLCYEAEQRSLSHADILDEQVIDPE
                     PYTGVLVIGVGRHQGQIDGLLSRFAKGWTIERMPALDRAILRVGMYELGHEPEVPTAV
                     VLNEAVELANSYSTDDSGRFVNGVLAAAARELRAD"
     gene            1547..2116
                     /gene="pyrR"
                     /locus_tag="PKY13_11635"
     CDS             1547..2116
                     /gene="pyrR"
                     /locus_tag="PKY13_11635"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOT80634.1"
                     /translation="MADRERRGTPPYGGEFQARTVVMDSVDLSRAIRRMAHEIVEHNH
                     GLDGVELIGLQTGGVPLAHAIAADLDEIEGVHLDVGVLDVAFYRDDIGIRPVLAEEVT
                     DIPFDVDAKTVVLVDDVLFTGRTIRAALDALGAYGRPRAVQLAVMIDRGHRELPIRPD
                     YVGKNLPTRRDEVVDVSSDGVHLGEMVRR"
     gene            2119..3060
                     /locus_tag="PKY13_11640"
     CDS             2119..3060
                     /locus_tag="PKY13_11640"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOT80635.1"
                     /translation="MRHLLSIADLERSDLESLLQLTDRFVEVSKRPIRNVPALRGKTV
                     VSLFFEDSTRTRLSFETAAKRLGADVMTFSVGTSSVKKGESLRDTVETISAFGIDAIV
                     VRHASSGVPHRIRQWTNAAILNGGDGWHQHPTQALLDSYTIRSHFGSLDGLRIAIVGD
                     VKHSRVARSDVQAFTKLGAEVVLVAPPTLLPPSLDGWPVTVSHDLDAVIGTIDVCYLL
                     RMQRERMVEALVPSLREYAHRFGLSPDRADRLGDRAIIMHPGPMNRGVEISPEAADRP
                     NAVVTEQVANGVAVRQAALFKLLGSESQFEAIEKAGS"
     gene            3057..4331
                     /locus_tag="PKY13_11645"
     CDS             3057..4331
                     /locus_tag="PKY13_11645"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOT80636.1"
                     /translation="MTIEYVIKGGRVIDASGETFADVLVRDGEIVGIGPDLNGSRTID
                     ATGCVVAPGLVDLHTHLREPGKEEAETIESGSNAAALGGYTAVLAMPNTTPAIDCASV
                     VRDVQAIARGVACDVEVAGAITVGRAGEQLAPMGEMSDLGVKIFTDDGTGVQDIRLMR
                     RALEYARGLGVTLAQHCEVSELSAGTCMHEGEVSSRLGMPGQPAEAEELMVMRDIALA
                     RMTGARMHFLHLSTAGSVAMVASAKAAGLPVTCEAAPHHFTLTDECVSSFDPVFKVHP
                     PLRTAGDVDAVRAGLLSGSIDAIATDHAPHPAYEKEKPFDEAPPGMLGLEYALALALT
                     ELGMDIAEVLARLSWKPAEIAGIADRHGAVAVGRVANLCVIDPNHEWTIDASGGASRS
                     TNVPYVGRTVRGKVRHTIHRGVPTVLDYELQS"
     gene            4530..>6108
                     /locus_tag="PKY13_11650"
     CDS             4530..>6108
                     /locus_tag="PKY13_11650"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT80637.1"
                     /translation="MIARLAAWCFRSRWKVIGAWFVLLVGVYAWGGAIGGDKYDGSFE
                     IPKSDSRSGLDVLEKSFGGGGAGLSGSIVYQVESGKVTDPAVQEPMTELFAAVAEINS
                     KEPDSDEHVSVVSPYEGEQGAAQISQDGRTAYATINLGSEVDQTESGKIGEKIEKLLP
                     TLDGVNIQIGGHALASFEPPSSELIGIAFAIVVLILAFGSVLAMGLPISVALGGVGVG
                     GGVVALVSHITSIPDLATIIGAMIGLGVGIDYALFIVTRYREGLHAGLSPQEATVTAL
                     DTAGRAVVFAGMTVVISLLGMMLIGLKFISGLGIGAAITVAATMLASVTLLPALLGLA
                     GPRVEVTRWRGLISAGFLAVALLGVGLKIDALLVGLPIAVVVFALGFAVAPLKATVRQ
                     RARKPTNETFAYRWSHTIQAHPWVSVLGGTVVLVVLALPLFSLHLGSSDDGNYAEDTS
                     TRKAYDLLADGFGPGFNGPLLVTASLNGPADAQAVTKLAADLAAADGVDKVVGPIPNN
                     FHPGYQPAEFAEAEAVLF"
BASE COUNT          926 a         2144 c         2063 g          975 t
ORIGIN      
        1 gcgcgaatgg tcgagagcgt cgccgcggcc cagcgggcgg gcgtcgagac cgtccgggcc
       61 ggcgcgacga gccgggaggt cgatcaggcc tgccgtgcgg tgatcgacga gtacggctgg
      121 gccgagcggt tcacccatgg caccggccat ggcgtcggcc tcgacatcca cgaggcaccg
      181 cgggtgtccg cggtgtcgga tgctacgctc gaacccggtt ttgtcgtgac cgtggagccc
      241 ggcgtctaca tcgctcccca cggcggcgtc cggatcgagg acaccgttgt ggtgaccgag
      301 accgggtgca cggtgctcac ggcgtcaccg aagacccttc tcgtggcctg atcctcgccg
      361 cgaacccgaa cccgcccgag cacgtacagc gacaggaact cccatggccc agatctccac
      421 cgccgacttc aagaacggca tcaccctcga cctgcccgag ggtctcgtcc agatcgtcga
      481 gttccagcac gtcaagcccg gcaagggcgg cgcgttcgtc cgcaccacgc tcaagaacgt
      541 tcgcaacggc gccgtcgtcg accgcacgtt ccgggccggc gagaagatgg agcgcgcctt
      601 catcgagaag aaggacatgc agttcctcta caacgacggc gccgactacg tgttcatgga
      661 caccacgagc tacgagcagc tgtcggtgcc gccggcctcg ctcggcgatt cgaagaacta
      721 cctgctcgag aacatgaccg ccgcggtgca gatgttcggt gacgagatca tcggcgtgga
      781 actgcccgcg gccgtcgacc tgccgatccg tgagaccgag ccgggtgtgc agggcgaccg
      841 ggtgtcgggt gcccgcaagc cggccacgct catcaccggc ctcgtcgtcc aggttccgct
      901 gttcgtcggc cccgatgaca tcatccgggt cgacacccgc acgggcgagt acatcacccg
      961 cgccaacgga tgaccgacat ctcgtcggcc gaggtcggtc ggcacgaggc acgtgagcgt
     1021 gccgtgcagc tctgctacga ggcggagcag cgcagcctga gccacgccga catcctcgac
     1081 gaacaggtca tcgatcccga gccgtacacc ggtgtcctcg tcatcggcgt gggccgccat
     1141 cagggccaga tcgacgggct gctcagccga ttcgccaagg gctggacgat cgagcggatg
     1201 ccggcactcg accgggccat cctccgcgtc ggcatgtacg agctgggcca cgagcccgag
     1261 gtccccaccg cggtggtgct caacgaggcg gtcgaactcg ccaacagcta ctcgaccgac
     1321 gactccggcc ggttcgtcaa cggcgtcctc gccgccgccg cccgcgagct ccgcgccgac
     1381 tgacgccgac ccgattccca ccccttctgt cctgcgattc cgcccctgga ggggcgaatc
     1441 ggcaggacag aaccggtgaa aatcggggct gctaacctca cggcgaccgt taacgggcgt
     1501 cccgagaggc gtccacggac cgcccttcgg ggcacgtgag gtgcacatgg ctgatcgcga
     1561 gcgtcgcggc acgccgccct atggcggcga gttccaggcc cgcaccgtgg tgatggactc
     1621 cgtcgacctg agtcgggcga ttcgtcgcat ggcccacgag atcgtcgagc acaaccacgg
     1681 gctcgacggg gtggagctca tcggactgca gacggggggc gtgccgctgg ctcacgccat
     1741 cgccgccgat ctcgacgaga tcgagggggt ccacctcgac gtcggggtgc tcgatgtcgc
     1801 gttctaccgc gacgacatcg ggatccgtcc cgtgctcgcc gaggaggtca ccgacatccc
     1861 cttcgacgtc gacgccaaga cggtggtcct cgtcgacgac gtgctcttca ccggccgtac
     1921 gattcgagcc gctctcgacg ccctcggtgc gtacgggcga ccccgagcgg ttcagctcgc
     1981 ggtcatgatc gaccgggggc accgggagct gccgatccgc cccgactacg tgggcaagaa
     2041 ccttccgacg cgccgcgacg aggtggtcga cgtgtcgtcc gacggtgttc acctcggcga
     2101 gatggtgcgg aggtagtcgt gcggcacctc ctgtccatcg ccgacctcga gcgatccgac
     2161 ctcgagtcgc tcctccaact caccgaccgg ttcgtcgagg tgtccaaacg cccgatccgc
     2221 aacgtgccgg ccctacgcgg caagacggtc gtgtcgctct tcttcgagga ctcgacccgc
     2281 acccgtctga gtttcgagac cgctgcgaag cgcctcggcg ccgacgtcat gacgttctcg
     2341 gtgggcacct cgtcggtgaa gaagggcgaa tcgctccgcg acaccgtgga gacgatctcg
     2401 gccttcggca tcgacgccat cgtcgtgcgc cacgcctcgt cgggcgtgcc ccaccgcatc
     2461 cggcagtgga ccaacgccgc catcctcaac ggtggcgacg gctggcacca gcatcccacc
     2521 caggcgctgc tcgacagcta cacgatccgg agccatttcg gctccctcga cggcctgcgc
     2581 atcgcgatcg tcggcgacgt caagcattcg cgggtcgccc gttccgacgt gcaggcgttc
     2641 acgaaactcg gcgccgaggt cgtgctcgtc gctccgccga ccctgctgcc gccgtcgctc
     2701 gacgggtggc ccgtcaccgt gtcccacgac ctcgacgccg tgatcggcac gatcgacgtc
     2761 tgctacctgc tgcggatgca gcgggagcgg atggtggagg cgctggtgcc gtcgctccgc
     2821 gagtacgcgc accggttcgg gctcagcccc gatcgggccg accggctcgg cgaccgggcg
     2881 atcatcatgc accccggtcc gatgaaccgt ggtgtggaga tctcgcccga ggccgccgac
     2941 cgtcccaacg ccgtcgtcac cgagcaggtg gccaacggcg tcgcggtgcg gcaggccgca
     3001 ctgttcaagc tgctcggcag cgagtcccag ttcgaagcga tcgagaaggc gggttcatga
     3061 ccatcgagta cgtgatcaag ggcggccggg tgatcgacgc gtcgggcgag acgttcgccg
     3121 acgtgctggt acgcgacggt gagatcgtgg gcatcgggcc cgacctcaac ggatcccgca
     3181 cgatcgacgc cacggggtgc gtcgtggcgc cggggctcgt cgacctgcac acccatctcc
     3241 gtgagccggg caaggaggag gctgagacca tcgagtccgg gagcaacgcc gccgccctcg
     3301 gtggctacac cgcggtgctc gccatgccga acaccacgcc cgccatcgac tgtgcgtcgg
     3361 tcgtgcgtga cgtccaggcc atcgcccgtg gcgtggcgtg tgacgtcgag gtggccggtg
     3421 ccatcacggt ggggcgcgcc ggcgagcagc tggccccgat gggcgagatg tcggatctcg
     3481 gcgtgaagat cttcaccgac gacggcaccg gggtgcagga catccggctc atgcgccggg
     3541 cgctcgaata cgcccgagga ctcggcgtga cgttggctca gcactgcgag gtgtccgagc
     3601 tcagcgccgg cacctgcatg cacgagggcg aggtgtcgtc gcgactgggg atgcccggcc
     3661 agcccgcgga ggccgaggaa ctcatggtca tgcgcgacat cgcgctcgcc cgcatgacgg
     3721 gggcgcggat gcacttcctg cacctgtcga cggccggctc ggtggcgatg gtggccagcg
     3781 ccaaggcggc cgggctgccg gtgacgtgcg aggcggctcc gcaccacttc accctgaccg
     3841 acgaatgcgt gtcgtcgttc gacccggtgt tcaaggtgca cccgccgttg cgcactgccg
     3901 gcgacgtcga cgcggtgcgc gccgggctgc tgtcgggttc gatcgacgcg atcgccaccg
     3961 atcacgcgcc gcatccggcg tacgagaagg agaagccctt cgatgaggcg cctcccggca
     4021 tgttgggcct cgagtacgcc ctcgccctgg cgctcaccga actgggcatg gacatcgccg
     4081 aggtgctcgc gcgcctgtcg tggaagccgg ccgagatcgc cgggatcgcc gaccgccacg
     4141 gtgcggtcgc ggtcggtcgc gttgccaacc tctgcgtgat cgaccccaac cacgaatgga
     4201 cgatcgatgc gagcggggga gcgagccgct ccaccaacgt gccctacgtg ggccgcaccg
     4261 tgcggggcaa ggtgcgccac acgatccacc gcggggtccc cacggtcctc gactacgagc
     4321 tgcagtcctg atcgccgggg gttttgtact gcgagaagcg ccgttgacgg ccttcctcgc
     4381 agtacaaagc cccgaggggt gggctcgcgg gcggacgtgg catgcgtcac aagtacgcat
     4441 tttgttggct gtggtgagca gtcgttccag tacggtggcg tctcgtaacg ggctgctggt
     4501 cgcgcgccca tgacgaacgg gagttcccca tgatcgctcg cctcgctgcc tggtgcttcc
     4561 gaagccgttg gaaggtgatc ggtgcgtggt tcgtcctgct cgtcggtgtg tacgcctggg
     4621 gcggcgccat cggtggcgac aagtacgacg ggagcttcga gatccccaag tccgacagcc
     4681 gctcgggcct cgacgtgctc gagaagagct tcgggggtgg cggtgccggc ctgagtggtt
     4741 cgatcgtgta ccaggtcgag tcgggcaagg tgaccgaccc ggccgtgcag gagcccatga
     4801 cggagctctt cgccgccgtc gccgagatca actccaagga gcccgactcc gacgagcacg
     4861 tcagcgtcgt cagcccctac gagggcgagc agggcgccgc tcagatcagc caggacgggc
     4921 gcacggccta tgccacgatc aacctcggct cggaggtcga ccagaccgag tcgggcaaga
     4981 tcggcgagaa gatcgagaag ctgctgccca ccctcgacgg ggtgaacatc cagatcggtg
     5041 gccacgccct ggcctcgttc gagccgccgt cgtcggagct catcggtatc gcgttcgcca
     5101 tcgtcgtgct catcttggcg ttcggctcgg tgctcgccat gggcctgccc atctcggtcg
     5161 ccctcggcgg tgtcggtgtc ggcggcggcg tcgtggcgct cgtgtcccac atcacgtcca
     5221 tccccgacct ggccacgatc atcggtgcga tgatcggcct cggcgtcggc atcgactacg
     5281 cgctgttcat cgtgacccgg taccgcgagg gcctgcacgc cggcctgtcg ccgcaggagg
     5341 cgacggtcac ggcgctcgac actgccggcc gagccgtggt gttcgccggc atgaccgtcg
     5401 tgatctcgct gctcggcatg atgctgatcg gcctcaagtt catctccggc ctcggcatcg
     5461 gcgcagcgat cacggtggcc gccacgatgc tcgcctcggt cacgctgctt ccggcgttgc
     5521 tcggtctcgc cggcccccgt gtcgaggtca cccgctggcg tggcctcatc tccgccgggt
     5581 tcctggcggt ggcgctgctc ggtgtcggcc tgaagatcga tgccctgctc gtcggtctcc
     5641 cgatcgccgt ggtcgtcttc gccctcggct tcgcggtcgc ccccctcaag gcgacggtgc
     5701 ggcagcgggc ccgcaagccc accaacgaga cgttcgccta ccgctggagc cacacgatcc
     5761 aggcgcaccc gtgggtgtcg gtcctcggcg gcaccgtcgt gctcgtcgtg ctggcactcc
     5821 cgctgttcag cctgcacctc ggtagctccg acgacggcaa ctacgccgag gacacgtcga
     5881 cccgcaaggc ctacgacctg ctggccgacg gtttcggccc cggcttcaac ggaccgctcc
     5941 tcgtgacagc gtccttgaac gggccggccg atgcccaagc ggtgacgaag ctcgccgctg
     6001 acctggcggc ggcggacggt gtcgacaagg tcgtcggtcc catccccaac aacttccacc
     6061 ccggctacca gccggcggag ttcgccgagg ccgaggccgt cctcttcc
//
LOCUS       DAOSOA010000195         6092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13057_length_6092_cov_5.932914, whole genome shotgun sequence.
ACCESSION   DAOSOA010000195 DAOSOA010000000
VERSION     DAOSOA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13057_length_6092_cov_5.932914"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PKY13_11655"
     CDS             <1..880
                     /locus_tag="PKY13_11655"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOT80638.1"
                     /translation="DLGAARYLRDGVVLPDETLEEWRSVDAILLGAVGDPAPGVAAPA
                     GLVERGILLKMRFELDMYINLRPFRLPPKINCEVIRENTEGTYAGEGGFLRKGTPLEV
                     ATQGSVNTRFGAERCIRYAFEKANHTERKTLHLIHKKNVLTFAGDLWQRTFDIVAAEY
                     PDVMTGYDHIDAACIHFVERPERYDVVVTDNLFGDILTDLGGAVAGGVGYAASANLNP
                     DRTGPSMFEPVHGTAPDIAGRNLANPIAAILSAAMMLDFLGETDAAERIQKACESPER
                     YTGSTTAVGDAVCAAL"
     gene            930..1844
                     /locus_tag="PKY13_11660"
     CDS             930..1844
                     /locus_tag="PKY13_11660"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOT80639.1"
                     /translation="MPITPTPKIWMNGELVDWDQANIHVLTHTLHYGMGVFEGIRAYE
                     TADGPGVFRLTEHIERLFRSAAILMMDIPYTVEEIVEATKETVRSSGLPGCYIRPIAY
                     YGYGEMGLNTLPCSVDVAIACWPWGAYLGDEAVSKGVRMKISSWTRHDHNTMPPASKT
                     TGNYVNSSLAKVEALKAGYDEAIMLNKTGLVSECTGENIFVARHGRFITPPLSAGALE
                     GITQSTVSSIATDLGFEVVEGELTRSDLYVADEMFVCGTAAEVSTVNSVDDRPIPCPG
                     PMSQAIADTYADVVRGKVDKYRSWVELA"
     gene            1841..3499
                     /gene="cimA"
                     /locus_tag="PKY13_11665"
     CDS             1841..3499
                     /gene="cimA"
                     /locus_tag="PKY13_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOT80640.1"
                     /translation="MSSDLPGLDLPNVGGEPEPVRAVEIFDTTLRDGAQFEGISLSVD
                     DKLRVAEQLDWLGVHWIEGGYPLSNPKDEEFFRRAPAELKLSTSTLVAFGSTRKPLGR
                     SDDDPTLRALVEAGTSTVCIVGKSSAFHVTEALGTTLEEGIAMAAESVAFLKAAGLRV
                     FFDAEHFFDGYKANPEYTLRVLEAAASNGADCLVLCDTNGGSLPHEVQRIVSEVASYF
                     EDTSLGIHTQNDSGCAVANSVAAVVGGVSQVQGTVNGYGERTGNANLMTVIPDLTLKM
                     GIATLPAGRLDRLTAVSRHVAELVNLPPHPADPYVGSSAFAHKGGLHTSALGKVGGSA
                     YEHIDPADVGNHTRVLVSDLGGRAGMSMKAQEFGVELDGRAAGELSERLKVLEAEGFV
                     FEAADASLELLMRRSTGWEHDFFSVEGYRVSSYHREGAVLAGSESPDLSTEATVKLWI
                     GDGQDAVRIASVGEGNGPVNALDAALRAALTDRYPALASIHLIDFKVRILDMSASTDA
                     VTRVLIDSTNGTDTWTTIGVSSNIIEASWMALVDALVFGLLKTD"
     gene            3577..4080
                     /locus_tag="PKY13_11670"
     CDS             3577..4080
                     /locus_tag="PKY13_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80641.1"
                     /translation="MAAPDYVPTSPTATKSYVSPRRRAGSWLADRPGELEGRQPEGDR
                     LGSPGPDQGYVVHLTDTLVGTLQLGSHEHESDALAAVAAVGLKRASLFGRAPVIHDVQ
                     AGVVVLGFDQAPPTGEAAERRRLRLEEAHHPHFYGKLRSIVDEIGPELLHRPLSAIEK
                     DGPQITW"
     assembly_gap    4276..4375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4394..5311)
                     /locus_tag="PKY13_11675"
     CDS             complement(4394..5311)
                     /locus_tag="PKY13_11675"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT80642.1"
                     /translation="MNSWGRRYRLPALAVALLLVAGAIVWAVTRAEPPKVAADPQPTT
                     TTSTPSTTTAPLPPIEVDPTRTDVATAVVPTIGVFAQPPADAVLDPRATAAGATLASL
                     VPGTSPERPNARAIPTIERPVIGRRANPTGWDFDNPTPWGNPLTFIVTEDHGEWLRVE
                     MPVRPNGTEGWIRASDVTRTIIDTHVEIDLSDRTLTAYAGDTVITTTKVVVGAADTPT
                     PTGRLYVTDYEEKYSGSAYGPWILPLSGYSQVMDEFSGGVPVIAMHGTNRPELIGTAA
                     SNGCIRMPDAVIEQLHASLPFGTPVDIRE"
     gene            5368..>6092
                     /locus_tag="PKY13_11680"
     CDS             5368..>6092
                     /locus_tag="PKY13_11680"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOT80643.1"
                     /translation="MRARGGAMATASEVHEVQTAKRRSAIRAARREREAARTDEQRRR
                     RRRLVRRGLLAGVVLVVLYLGFTVAQVFAADSWDHTRDADAAIVLGAAQYNGTPSPVF
                     KGRLDRAFDLWKEGRVPLIVVTGGAAEGDRFTEAYSGLTYLKKKGVPDDDMIVIDDGS
                     STWESMAASVRVLRKQGITKVQLVSDPYHSFRLEAIADEVGLDGEVSPTSADSTRGEL
                     LRESMLVAGGRIIGYRRLVNLVE"
BASE COUNT          908 a         2036 c         2089 g          959 t
ORIGIN      
        1 cgacctgggt gccgcccgct acctgcgcga cggtgtggtg ctgcccgacg agacgctcga
       61 ggagtggcgg tcggtcgacg cgatcctgct cggtgcggtg ggcgacccgg cacccggtgt
      121 cgccgccccg gccggcctcg tcgagcgggg gatcctgctc aagatgcgct tcgagctcga
      181 catgtacatc aacctccgcc cgttccggct gcctccgaag atcaactgcg aggtcatccg
      241 ggagaacacc gagggcacct acgccggcga gggtgggttc ctgcgcaagg gcacaccgct
      301 cgaggtggcc acgcagggct cggtcaacac ccgcttcggc gccgagcgct gcatccgcta
      361 cgcgttcgag aaggccaacc acaccgagcg caagacgctg cacctgatcc acaagaagaa
      421 cgtgctcacg ttcgccggcg acctctggca acgcacgttc gacatcgtgg ccgccgagta
      481 ccccgacgtc atgaccggct acgaccacat cgacgccgcg tgcatccact tcgtggagcg
      541 cccggagcgt tacgacgtcg tcgtcaccga caacctgttc ggtgacatcc tcaccgacct
      601 cggtggcgcc gtcgccggcg gcgtcggcta cgcggcctcc gccaacctca accccgaccg
      661 caccgggccg tcgatgttcg aaccggtcca cggcacggcc cccgacatcg ccgggcggaa
      721 cctggccaac ccgattgcag cgatcctgag cgccgcgatg atgctcgact tcctcggcga
      781 gaccgacgcc gccgagcgca tccagaaggc ctgcgaatcg cccgagcgct acaccggttc
      841 caccaccgcc gtcggcgatg ccgtgtgcgc ggcgctgtag ccctcaccac caccacccga
      901 atcccagtcg aacagaaaga gcacacgaga tgcccatcac ccccacgccc aagatctgga
      961 tgaacggcga actggtcgac tgggaccagg ccaacatcca cgtgctcacg cacacgctgc
     1021 actacggcat gggcgtgttc gagggcatcc gggcctacga gacggccgac ggtcccggtg
     1081 tgttccggct gaccgagcac atcgagcggc tcttccgttc ggcggcgatc ctcatgatgg
     1141 acattccgta cacggtcgag gagatcgtcg aggcgacgaa ggagacggtg cggtcgtcgg
     1201 ggctcccggg ctgctacatc cgtccgatcg cgtactacgg gtacggcgag atggggctca
     1261 acacgctgcc gtgcagcgtc gacgtcgcga tcgcgtgctg gccgtggggc gcgtacctcg
     1321 gcgacgaggc ggtctcgaag ggcgtgcgca tgaagatcag ctcgtggacg cgccatgacc
     1381 acaacacgat gccgccggcg tcgaagacga cgggcaacta cgtgaactcg tcgctcgcca
     1441 aggtcgaagc cctcaaggcc ggctacgacg aggcgatcat gctcaacaag acgggcctgg
     1501 tctcggagtg caccggtgag aacatcttcg tggcccgcca cggccggttc atcacgccgc
     1561 ccctctcggc gggtgccctc gagggcatca cgcagtcgac ggtgtcatcg atcgccaccg
     1621 acctcgggtt cgaggtcgtg gagggtgagc tgacgcgcag cgacctgtac gtcgccgacg
     1681 agatgttcgt gtgcggcacc gctgccgagg tgtcgacggt caactcggtg gacgatcgcc
     1741 cgatcccgtg ccctggcccg atgagccagg ccatcgccga cacctacgcc gacgtcgtcc
     1801 gcggcaaggt cgacaagtac cgcagttggg tggaactggc gtgagttccg acctgcccgg
     1861 cctcgacctg cccaacgtcg ggggcgaacc cgagccggtg cgggccgtcg agatcttcga
     1921 cacgacgctg cgcgacggtg cgcagttcga gggcatctcc ttgtcggtcg acgacaagtt
     1981 gcgcgtcgcc gagcagctcg actggttggg cgtgcactgg atcgagggcg gctatccgct
     2041 gtcgaacccg aaggacgagg agttcttccg tcgtgcccct gccgagctga agctgtcgac
     2101 gtcgacgctc gtcgcgttcg gctcgacccg caagccgttg ggccgctccg acgacgaccc
     2161 gacgttgcgt gctctggtcg aggccggcac gtcgacggtc tgcatcgtgg gcaagtcgag
     2221 cgcgttccac gtgaccgagg cgctgggcac gacgctcgag gagggcatcg cgatggccgc
     2281 cgagtcggtc gcgttcctca aggccgccgg cctccgggtg ttcttcgacg ccgagcactt
     2341 cttcgacggg tacaaggcga accccgagta cacgctgcgt gtgctcgagg cggccgcttc
     2401 caacggcgcc gactgcctcg tgctgtgcga caccaacggc ggatcgttgc cccacgaggt
     2461 gcagcgcatc gtgtccgagg tggcgtcgta cttcgaggac acgtcgctcg gtatccacac
     2521 ccagaacgac tcgggctgcg cggtcgccaa ctccgtcgcc gccgtcgtcg gtggcgtctc
     2581 gcaggtgcag ggcaccgtca acggctacgg cgagcgcacc ggcaacgcca acctcatgac
     2641 ggtcatcccc gacctcacgc tcaagatggg catcgcgacg ctccccgctg gccgcctcga
     2701 tcgtctcacc gcggtctccc gccacgtcgc cgagttggtc aaccttccac cgcaccctgc
     2761 cgacccctac gtcgggtcgt cggcgttcgc ccacaagggc ggcctgcaca cctctgccct
     2821 cggcaaggtg ggtgggtcgg cgtacgagca catcgatccc gccgacgtcg gcaaccacac
     2881 gcgggtgttg gtctccgacc tcgggggtcg cgccggcatg tcgatgaagg cccaggagtt
     2941 cggggtcgag ctcgacgggc gcgccgccgg cgagctgtcg gagcggttga aggtcctcga
     3001 ggccgagggg ttcgtgttcg aagccgccga tgcgtcgctc gagctgctca tgcgtcggtc
     3061 gacggggtgg gagcacgact tcttctccgt cgagggctat cgcgtgtcgt cctaccaccg
     3121 tgagggggcg gtactcgctg ggagcgagtc gcccgacctg tcgacggagg cgaccgtcaa
     3181 gctgtggatc ggcgacggtc aggatgcggt gcggatcgcg tcggtggggg agggcaacgg
     3241 gccggtgaac gcgctcgatg cagcgctccg cgccgcgctc accgatcgct acccggcgct
     3301 cgcgagcatc cacctcatcg atttcaaggt gcggatcctc gacatgtcgg cgagcaccga
     3361 cgccgtgacc cgggtcttga tcgactcgac gaacggcacc gacacctgga ccacgatcgg
     3421 cgtgtcctcg aacatcatcg aggcgtcctg gatggcgctc gtcgacgcgt tggtgttcgg
     3481 cctgctcaag accgactgac cgccgttctg ccctgcaaac cgtacggttc gccgattgaa
     3541 atgcaggaca gagctggccg gtaggctggc ggtcgtatgg ctgcacctga ttacgtcccc
     3601 acctcgccga ccgcgaccaa gtcgtacgtg tcgccccgcc gccgcgccgg gtcgtggctg
     3661 gccgaccgtc cgggcgagct cgaaggccgt cagccggagg gcgatcgcct gggcagcccc
     3721 ggtcccgacc agggctatgt cgtgcacctc accgacacgc tcgtcggcac gctgcagctc
     3781 ggcagccacg agcacgagtc ggacgcgttg gccgccgtcg cggccgtcgg tctcaagcgg
     3841 gcgtcgctct tcgggcgtgc gccggtgatc cacgatgtgc aggcgggcgt tgtggtgctc
     3901 ggcttcgacc aggcgccgcc gaccggcgag gccgccgaac gtcgccggct gcggctcgaa
     3961 gaggcgcacc atccgcactt ctacggcaag ctccgcagca tcgtcgacga gatcggtccc
     4021 gagctgttgc accgtccgct ctcggcgatc gagaaggacg gcccccagat cacgtggtga
     4081 tcgttcgggt ttccggccgc tgaggccgta tgcaggccgt gtgggcggtc cagggagccc
     4141 tggccgtcat cttcaccgcc gggttgtttc ccctggcgct gctcccgctg gtcaacgggg
     4201 tctaccggaa cttcgggcgt ttcgccggct ggcccgcggt cgtcgcgttc tcgtcgttgg
     4261 caatgggatg cgcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacgag
     4381 gtcgtgggcc gcgtcactcg cggatgtcga cgggcgttcc gaaggggagt gaggcgtgca
     4441 gctgctcgat cacggcgtcg ggcatgcgga tgcacccgtt ggaggccgcc gtgccgatga
     4501 gctcgggacg gttcgtgccg tgcatggcga tcaccggaac gccgccgctg aactcgtcca
     4561 tgacctggga gtagcccgag agcggcagga tccaggggcc gtatgccgag ccggagtact
     4621 tctcctcgta gtcggtcacg tagaggcgac cggtcggggt gggggtgtcg gcagcgccga
     4681 cgaccacctt cgtcgtggtg atcacggtgt cgccggcgta ggccgtcaac gtgcggtcgc
     4741 tcaggtcgat ctccacgtgg gtatcgatga tcgtgcgagt gacgtcgctg gcgcggatcc
     4801 agccctcggt gccgttggga cgaacgggca tctcgacgcg gagccactcg ccgtggtcct
     4861 cggtgacgat gaacgtgagc gggttgcccc agggagtggg gttgtcgaag tcccaaccgg
     4921 tcgggttggc gcgacgcccg atgacggggc gttcgatcgt ggggatcgcc cgggcgttcg
     4981 gccgttcggg tgaggtgccg gggacgaggc tcgccagggt ggcgccggcc gctgtcgccc
     5041 gcgggtcgag cacggcgtcg gcgggcggct gcgcgaacac cccgatcgtg gggacgacgg
     5101 cggtggcgac gtcggtacgg gtggggtcga cctcgatcgg ggggagcggc gccgtggtcg
     5161 tactcggtgt gctggtcgtt gtggtgggct gcggatcggc cgccaccttc ggtggttcgg
     5221 cccgggtgac cgcccacacg atcgctccgg cgaccagcag cagcgccacc gccagcgcgg
     5281 gcagtcggta ccgtcgtccc caggaattca cccgatcagc ttacggccgc ctggtccgat
     5341 ccgatcagcg cccgcttgta ccctgagatg cgagctcggg gaggagcgat ggcgacggcg
     5401 agcgaagtgc acgaggtcca gaccgccaag cggcggtcag cgatccgtgc cgcccgtcgg
     5461 gagcgcgaag ccgcccgcac cgacgagcag cgccgtcgac gccgtcgcct cgttcgccgc
     5521 ggcctcctcg ccggggtcgt gctcgtcgtg ttgtacctcg ggttcaccgt ggcgcaggtg
     5581 ttcgccgccg actcgtggga ccacacccgt gacgccgatg ccgccatcgt cttgggcgcg
     5641 gcccagtaca acggcacccc gagtccggtg ttcaagggac gactcgaccg cgccttcgac
     5701 ctgtggaagg aagggcgggt cccactcatc gtcgtcaccg gtggtgccgc cgagggcgac
     5761 cggttcaccg aggcctactc ggggctcacc tacctgaaga agaagggcgt gcccgacgac
     5821 gacatgatcg tcatcgacga cgggtcgagc acctgggagt cgatggcggc gtcggtccgg
     5881 gtgctccgca agcagggcat cacgaaggtg cagctggtgt cggacccgta ccactcgttc
     5941 cggctcgagg cgatcgcgga cgaggtggga ctcgacggcg aggtgtcgcc gacctcggcc
     6001 gactcgacga ggggcgagct cctgcgggaa tcgatgctcg tcgccggtgg ccgcatcatc
     6061 gggtaccgcc gcctcgtgaa cctcgtcgag ta
//
LOCUS       DAOSOA010000196         6024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13289_length_6024_cov_5.310940, whole genome shotgun sequence.
ACCESSION   DAOSOA010000196 DAOSOA010000000
VERSION     DAOSOA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13289_length_6024_cov_5.310940"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKY13_11685"
     CDS             <1..454
                     /locus_tag="PKY13_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOT80644.1"
                     /translation="GSRLVGLGRAAAAALPLAELAEPVLAELRDLSGESAQLYVRQGG
                     HRVCVAALDSPHELRTIVAPGATLPIGVGSGGRLLSGEAPSRGGWIESVEDRAPGVAS
                     VSAPVLDRAGTIVAAISVSGPIDRIGRSPGKRHGAEVVAAARALEALL"
     gene            complement(456..1481)
                     /gene="rsgA"
                     /locus_tag="PKY13_11690"
     CDS             complement(456..1481)
                     /gene="rsgA"
                     /locus_tag="PKY13_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT80645.1"
                     /translation="MTNHDTLRPLGWNEHVADVVAPLLTDDHLEPGRIVRVDRGEVDV
                     AVGVGPDNVIRATNTTSSKDCVAGDWVVLDRAQARVEAVAPRLTAFTRRSARGARVAQ
                     TLAANMDVVLVVQGLDPGVNVRRLERELVLAHQSGATPIVVLTKTDAVDAAFIESSLA
                     AARRSAPGVEVVAVSNRDRSGFDQLDRLVRPGRTFALLGSSGVGKSTLVNSFAGETIM
                     LEGEIRDGDGKGRHTTTAARLLHLADGRLLLDTPGTRSLALWESWEGLADTFPEVETA
                     AEDCQFADCFHENEPGCAVIAAVADGTIPADRLDSWRHLREEMEQLDGHLVDQQRKAE
                     RTGRPPR"
     gene            1493..3127
                     /locus_tag="PKY13_11695"
     CDS             1493..3127
                     /locus_tag="PKY13_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOT80646.1"
                     /translation="MTDRVHPSVDATAGTEVERPSSDPRRSVPDVLAAIDIGTNSVHM
                     VVARMAPDGRFEVITRHKEMVRLGESGDDQLKHLTPGAIDRGVAALARCRSVVDTYGA
                     PVEAVATSAVREAENRNDFIRRAQDEAGIDVEVIAGHEEARLIHLGVLQALPLYDTPL
                     ILCDIGGGSTELLIGHRGEVQVSRSFKLGAIRMTQRFFDGGVTSKRSVERARRFVRAT
                     LAPFVREAAAHPFEVAVGSSGTIESLLGIARAAEGSVKQSVNGAVLTRAALGEVIERL
                     IAAETPEARAEIKGVDKGRADIIVGGAVILEQVMDSLNIESLVISEYALREGVLFDLN
                     NRLHGASLDHLSDLRRRSIEHLMEVCDEDPDHSLQVAELALELFDGLEGRHGLGHDER
                     ELLEAAALLANVGLFIAHSRHHQHSYYVIRNSELLSGFTDPEIEIIAQVARYHRRGEP
                     SLKHAPFAALDEHDQRRVRWLAAILRVAIGLDRSHAGAVATLGVGDAVVGGDDAASVM
                     ITVVPNGDADISLEIYSARERVGLLEQMLEQAVQVR"
     gene            complement(3161..3532)
                     /gene="mscL"
                     /locus_tag="PKY13_11700"
     CDS             complement(3161..3532)
                     /gene="mscL"
                     /locus_tag="PKY13_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOT80647.1"
                     /translation="MFAGFKKFILQGNVIDLAVGVVIGAAFGALVKAFTDAFITPLIA
                     AITGGGTTGGTFTINDQVFNYGAFISAIITFVLTAAVVYFVIVAPMNILKDKRAKGVE
                     ADVEPSNEEKMIALLEQIAGK"
     gene            complement(3624..4097)
                     /gene="greA"
                     /locus_tag="PKY13_11705"
     CDS             complement(3624..4097)
                     /gene="greA"
                     /locus_tag="PKY13_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT80648.1"
                     /translation="MSESEYQLSQAAYDRLKAELDELTTVGRINIARKIEVAREMGDL
                     SENGDYHAAKEEQGKMEGRIGHLAALIERSTIISEDGPLDAVAQGCVVTIVYEGDDEE
                     EQFLIGSIEERRDGVEVISPTSPLGGALLGAKAGEKVTFEAPTGELTVEVVRFER"
     gene            complement(4250..5671)
                     /locus_tag="PKY13_11710"
     CDS             complement(4250..5671)
                     /locus_tag="PKY13_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT80649.1"
                     /translation="MTNTSSIPMHGGHSGQADVAALLRTCISTLETVMADLDPAAVPL
                     PHVTGLWKLFSRAQKLSAAGELSLADQVRRADEWRRSGYQSAEEWMARQKGTSVGDAR
                     GRLEASKHLEALPDTAAALRAGLVSPEQAVIVADAAIANPAAEADLLRQAGRDSHSGL
                     RREAARRKAQALPDGEERARRHHRERKANVWTDAEGRCNLAAQGPLSASAGFVAWLER
                     ETDRRFREAQRAGAREPREAYMFDALMSLSESGPGPESGNDRRRVAPKHLALIRVDLT
                     ALRRGSVAGDELCEIGGLGPVSVSEARALLGDSILHLVITKGQAVQSITHLGRGPNAV
                     QQLALLWQSETCSVLGCARTRVEHDHRIEFNETRHTRLDELDPLCTFHHRQKTHRGWA
                     LVAGSGDRDFVPPDDARHPARIADGRRSAVPPRAPAPTPAAPGVSPELAARLCRVRAK
                     IEERTRDHPDVAEFLSRVNAYLD"
BASE COUNT          845 a         1986 c         2184 g         1009 t
ORIGIN      
        1 cggctcccgg ctcgtcggcc tgggtcgggc cgcagcggcg gcactgccgt tggccgagct
       61 ggccgagccg gtcctggccg agctccgtga cctctcgggg gagtcggccc agctgtacgt
      121 ccggcagggc ggccaccggg tgtgcgtcgc tgcgctcgac tcgccgcacg agttgcggac
      181 gatcgtggcg cccggtgcga cgctgcccat cggtgtgggg tcgggtgggc gcttgctgtc
      241 gggggaggcc ccgagccggg gcggctggat cgaatcggtc gaggaccgtg cgcccggagt
      301 cgcatccgtg tcggcgccgg tactcgatcg tgccggaacg atcgtcgctg ccatcagcgt
      361 gtccggtccg atcgaccgca tcggtcgcag ccccggcaag cgccacggtg ccgaggtcgt
      421 cgcggcggcc cgcgccctcg aagccctcct ctgagtcagc ggggtggccg gccggttcgc
      481 tcggccttgc gctgctggtc gacgaggtgc ccgtcgagtt gctccatctc ctcgcggagg
      541 tggcgccagc tgtcgagacg atcggcgggg atcgtgccgt ccgccaccgc ggcaatcacc
      601 gcgcagcccg gttcgttctc gtggaagcag tcggcgaact ggcagtcctc cgcggctgtc
      661 tcgacctcgg gaaacgtgtc tgcgaggcct tcccacgatt cccacagcgc cagcgaccgg
      721 gtgccgggtg tgtcgagcag gagccgcccg tcggcgaggt ggaggagccg tgccgcggtg
      781 gtggtgtgac ggcccttgcc gtcgccgtcg cggatctcgc cctcgagcat gatcgtctcg
      841 ccggcgaagc tgttgacgag cgtggacttg ccgacgccgg acgacccgag cagcgcgaat
      901 gtccgcccgg gccgcaccag gcgatcgagt tggtcgaagc cggaccggtc gcggttggag
      961 accgccacca cctccacgcc gggggcgctc cgacgcgccg ccgccagcga ggactcgatg
     1021 aacgcagcgt cgaccgcgtc ggtcttggtg agcacaacga tcggggtcgc cccgctctgg
     1081 tgggcgagca cgagttcccg ctcgagccgt cgcacgttga cgccggggtc gaggccctgg
     1141 acgaccagga ccacgtccat gttcgctgcg agggtctggg ctacccgggc gccacgagcc
     1201 gaacggcggg tgaacgcggt gagtctcggc gcaacggcct cgacccgcgc ctgggcccga
     1261 tcgagcacga cccagtcgcc ggcgacgcag tccttcgacg acgtggtgtt cgtggcccgg
     1321 atcacgttgt cgggcccgac gcccactgcg acgtcgacct cgccgcgatc gacgcggacg
     1381 atgcggccgg gctcgaggtg atcgtcggtc agaagtggcg ccacgacgtc ggcgacgtgc
     1441 tcgttccaac cgagtggccg gagggtgtcg tggttggtca tacgctcggg aggtgactga
     1501 tcgggtacat ccttccgtcg acgctaccgc cgggaccgag gtggagcggc cgagttccga
     1561 tccacggcgc tcggtgcccg acgtgttggc ggcgatcgac atcgggacga actcggtcca
     1621 catggtcgtg gcgcgcatgg cacccgacgg ccggttcgag gtcatcaccc gccacaagga
     1681 gatggtgcgg ctgggtgagt ccggcgacga ccagctcaag cacctcaccc cgggcgccat
     1741 cgatcgtggg gtggcggcgc tcgctcggtg tcggagtgtg gtcgacacgt acggcgcccc
     1801 cgtcgaggcg gtggccacgt cggcggtgcg ggaggccgag aaccgcaacg acttcatccg
     1861 gcgggcgcag gacgaggcag gcatcgacgt cgaggtcatc gccggccacg aggaggcccg
     1921 gctcatccac ctcggcgtgc tgcaggcgct gccgctctac gacaccccgt tgatcctgtg
     1981 cgacatcggt ggcggcagca cggagctctt gatcggtcac cggggcgagg ttcaggtgag
     2041 ccgcagcttc aagctgggtg cgatccgtat gacccagcgg ttcttcgacg gcggggtgac
     2101 gtcgaaacgg tcggtcgagc gggcccgccg gttcgtccgc gccacgctcg cgccgttcgt
     2161 gcgcgaggcc gcagcacacc cgttcgaggt ggcggtcggc agctcgggga ccatcgagag
     2221 tctcctcggc attgctcggg cagccgaggg ttcggtcaag cagtcggtca atggtgccgt
     2281 cctgacccgg gcggcgctcg gcgaggtgat cgagcggttg atcgctgcgg agaccccgga
     2341 ggcccgagcc gagatcaagg gggtcgacaa ggggagggcc gacatcattg tcgggggtgc
     2401 ggtcatcctc gagcaggtga tggactcgct caacatcgag tcgctcgtca tcagtgagta
     2461 cgcgctccgc gagggcgtgt tgttcgacct caacaatcgg ttgcacggtg cgtcgctcga
     2521 ccatctctcg gacctgcgtc gccgcagcat cgagcacctg atggaggtgt gcgacgagga
     2581 tcccgaccac tcgctccagg tggcggagct cgcgctcgaa ctcttcgacg ggctcgaagg
     2641 tcgtcacggt ctcggccacg acgaacgcga gttgctcgag gcggcagcgc tgctcgcgaa
     2701 cgtgggcttg ttcattgccc actcccgtca ccaccagcac agctactacg tgatccggaa
     2761 ctccgagctg ttgagcgggt tcaccgaccc cgagatcgag atcatcgccc aggtcgcccg
     2821 ctatcaccgg cgaggagagc cctccctgaa gcacgctccg ttcgctgcgc tcgacgagca
     2881 cgaccaacga cgggtgcggt ggctcgccgc gatccttcgg gtcgccatcg gcctcgaccg
     2941 cagtcacgcc ggagcggtcg caaccctggg ggtgggcgat gcggtcgtcg gcggcgacga
     3001 cgctgcctcg gtgatgatca cggtggtgcc caacggtgat gccgacatct cgctcgagat
     3061 ctactcggcc cgggaacggg tcggcctgct cgagcagatg ctcgagcagg ccgttcaggt
     3121 ccgctgacgt tcggcgccgg ccccagctcc ggctggtcga ctacttgccg gcgatctgct
     3181 ccagcagggc gatcatcttc tcctcgttgg agggctcgac gtcagcctcg acgcccttgg
     3241 cgcgcttgtc cttgaggatg ttcatggggg cgacgatcac gaagtagacg actgccgcgg
     3301 tcagcacgaa cgtgatgatc gcgctgatga acgccccgta gttgaagacc tggtcgttga
     3361 tcgtgaacgt accgccggtt gttccgccgc cggtgatcgc tgcgatcagc ggggtgatga
     3421 aggcgtcggt gaacgctttg acgagcgcgc cgaacgcggc gccgatgacg acgccgacgg
     3481 cgaggtcgat gacgttgccc tgcaggatga acttcttgaa accagcgaac atggggatat
     3541 gcctttcgtc tcggtgccgg gggtgcccga caccgctgcg aaagatatcc ccgatcgttt
     3601 ccccgaggtc gggatcagcg cgctcagcgc tcgaagcgga cgacctcgac ggtgagctcg
     3661 ccggtcggcg cttcgaacgt caccttctcg ccggcttttg cgccgagcag cgcaccgccg
     3721 agcggggagg tgggggagat gacctcaacg ccgtcgcggc gctcctcgat cgaaccgatg
     3781 aggaactgct cctcctcgtc gtcgccctcg tacacgatgg tgacgacgca tccctgggcg
     3841 acggcgtcga gcgggccgtc ctcgctgatg atggtcgaac gctcgatcag ggcggcgagg
     3901 tggccgatgc ggccttccat cttgccctgc tcttccttgg cggcgtggta gtcgccgttc
     3961 tccgacaggt cgcccatctc gcgggcgacc tcgatcttgc gggcgatgtt gatgcgtccc
     4021 accgtcgtca gctcgtcgag ctcggccttg agccggtcgt acgcggcttg tgaaagttgg
     4081 tactcggact cactcatggc ggacgagcct atcgttcgcc atgacgaatg caccgagccc
     4141 cgctgtgggc gtgggcacgc gcgactgcta cccggacact cgtcggggtg gtgatcgcga
     4201 tgtgatcggc agtgtgatcg acgttgtgac cggggccccg gttcggaact cagtcgagat
     4261 acgcgttgac ccgggacagg aactcggcca cgtcggggtg gtcgcgggtt cgctcttcga
     4321 tcttcgcccg gactcggcac aggcgggcgg cgagctcggg actcacgccg ggtgccgcag
     4381 gtgtgggtgc gggtgcgcgt ggcgggactg ccgatcgtcg accgtccgcg atgcgggcag
     4441 ggtgccgggc gtcgtcgggc gggacgaagt cacggtcacc gctgccggcc acgagcgccc
     4501 aacctcggtg cgtcttctgg cggtgatgga acgtgcacag gggatcgagt tcgtcgagcc
     4561 gggtgtgcct ggtctcgttg aactcgatgc gatggtcgtg ttcgacgcgg gtgcgagcgc
     4621 acccgagcac cgagcacgtc tcggactgcc acaggagtgc aagttgctgc accgcgttgg
     4681 ggccacgacc gaggtgcgtg atcgactgca ccgcttggcc cttggtgatc acgaggtgaa
     4741 ggatcgagtc gccgaggagc gcccgcgcct cgctcaccga gacgggcccc agcccgccga
     4801 tctcgcagag ctcgtcgccg gcgacgctgc cacgtcggag cgccgtgaga tcgacccgga
     4861 tcagggccag gtgcttgggc gccacccgcc ggcggtcgtt gcccgactcc ggtcccggcc
     4921 ccgactccga gagcgacatc agcgcgtcga acatgtaggc ctcacgaggt tcgcgtgcac
     4981 cagctcgttg cgcctcgcgg aagcgtcgat cggtctcgcg ttcgagccat gccacgaacc
     5041 cggcggatgc ggacagtgga ccctgggcgg cgaggttgca ccgcccttca gcgtcggtcc
     5101 agacgttggc cttgcgttcg cggtggtggc gccgggcgcg ttcctcgccg tcggggagcg
     5161 cttgcgcctt gcggcgggct gcctctcgcc ggaggccgct gtgggagtcg cgtcctgcct
     5221 gacgcaacag gtcggcctcg gcggccgggt tggcgatcgc ggcatcggcg acgatgacgg
     5281 cttgttcggg cgacacgagg ccggctcgca gtgcggcggc ggtgtcgggg agcgcttcga
     5341 ggtgtttgga cgcttcgagg cggccgcggg cgtcgccgac cgaggtgccc ttctggcggg
     5401 ccatccactc ctcggcggac tggtaccccg accgccgcca ctcgtcggcc cggcggacct
     5461 ggtcggcgag gctgagctcg cccgcggcgc tcagcttctg cgcacgggag aacaacttcc
     5521 agaggcccgt cacgtggggg agcggcacgg cggcggggtc gagatccgcc atgacggtct
     5581 cgagcgtcga gatgcaggtg cggagcaggg ccgccacgtc tgcctgcccg gagtggcctc
     5641 cgtgcatggg tatcgaactg gtgttcgtca cgactccatc gtgtcacgct cgaacggggt
     5701 gcgagaccga aactcgggga tcgggaagaa tcacccatgg gtcgccgccg gttcggcagg
     5761 ggttccgggc aggtcgccgc agccggatgg ctctgacggg ctcaccagac cccggtagtc
     5821 tcgtcgggtc ctggttcgct tcgagagcct gcctgtctgg agtactgccc atgagttcac
     5881 atcgcatcgc cgtcgtcggt ggcgacggca tcggccccga ggtcactgcc gaggccctca
     5941 aggtcatcga tgccgtcggc gtctcctacg agcgcatcga ctacgacctg ggtgccgccc
     6001 gctacctccg cgacggcgtg gtgc
//
LOCUS       DAOSOA010000197         6004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13350_length_6004_cov_5.484283, whole genome shotgun sequence.
ACCESSION   DAOSOA010000197 DAOSOA010000000
VERSION     DAOSOA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13350_length_6004_cov_5.484283"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKY13_11715"
     CDS             complement(<1..262)
                     /locus_tag="PKY13_11715"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOT80650.1"
                     /translation="MTTLAERLGYAADDRLLIINCDDLGMSHAANEGCFSALRDGIAT
                     SATLMVPCPWARAAVAESDGEDIGVHLTLNSEYDRYRWGPITH"
     gene            complement(296..1393)
                     /gene="ppk2"
                     /locus_tag="PKY13_11720"
     CDS             complement(296..1393)
                     /gene="ppk2"
                     /locus_tag="PKY13_11720"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOT80651.1"
                     /translation="MTDTRTVDSGDRAPDLSAEAIAAFEAEEGADSAAGSTINAVRDL
                     LRGARADDLQEVARWLQALIEGQSPDELTQLGTLLAQDEPLWGSPPDPDDVMLSDGWQ
                     SGVYPYRNLMSRKAYEREKYQLQVELLKLQAWCKDTGERLVIVFEGRDAAGKGGTIKR
                     FMEHLNPRGARVVALEKPTDAERGQWYFQRYVQHLPTAGEIVLFDRSWYNRAGVERVM
                     GFCSDEEYREFLQQAPSFEQSLARSGVRLLKFWFSVSREEQLRRFHERERHPLKQWKL
                     SPVDLASLDKWDDYTLAKETMFLHTDTAMAPWTVIKSDCKKRARLNAMRFVLHQVPYA
                     EKDVERIGTLDPLIVGRADLLNDFSERPATL"
     gene            complement(1464..2324)
                     /gene="mca"
                     /locus_tag="PKY13_11725"
     CDS             complement(1464..2324)
                     /gene="mca"
                     /locus_tag="PKY13_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HOT80652.1"
                     /translation="MTDAPALRLLSVHAHPDDEASKGAALVARYVGEGAHATLVCCTG
                     GELGSILNPAMDRPEVVENLPEVRAKELERSAQIIGYQRVEMLGYKDSGMPDMPENAD
                     PANFANAPLDDAVLRLVRIIRRDRPQVLLTYDEEQGHYPHPDHLRTHEITVHAFDRAG
                     DPDWFPEAGDPWQPSKMYYTTWARARIEATHQKMLELGIESPFPKDWFDRPNQDHRIT
                     TKIDVGAFLHIRDEALLAHATQVDPNEKFWFGLTPQIAAATYPWDDYILARSTIDTDL
                     PEDDLFAGLR"
     gene            complement(2383..3591)
                     /locus_tag="PKY13_11730"
     CDS             complement(2383..3591)
                     /locus_tag="PKY13_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOT80653.1"
                     /translation="MLAEAKDTSELMVDLAYAAVYFNDPGMCEEVAALESRLNDLVQD
                     MRAVCILAVRRPSEAESMAAVLQVIGAIEGIGNAAIDITRIVTHRLGIPNELIGDLSN
                     AEEVSHRVWIREGSHMANRPVAALELPVATGMRIMAIRRGRSWISEIDGDLVLLPGDV
                     LFLRGSPPGITRLHELAAAPTWSPPSVPAPGALTDLDRAVDVLVEMKNISEASVGLAY
                     SALALRDRGLAAEVVHLANRLDEMKDHLQLWVLRASASNVDPSPLRGLLQLSHVAEEL
                     GDQASQMVWLISDEIEFHPVLAMALGESDVVVMRVPVADGAAVVGKSMADMQLDIEPG
                     FHVLALLRRGQYLYRPRPFVSFEAGDEVIASGPHEGRQRLAELFGWKLLEGDDDDALG
                     EPELVPLRSA"
     gene            3788..4894
                     /locus_tag="PKY13_11735"
     CDS             3788..4894
                     /locus_tag="PKY13_11735"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT80654.1"
                     /translation="MPRRSSVLLLAILLLTTAGCIRAKPGARCNSTDWGENGTHVMRC
                     VGGRWKIVMTRQQAIDALVALSEAAKKPPVGDVVRPLDLGPANLPNTADPSVFVENGV
                     SYVFSTSTYQHVPVATMSDIDAPLAMYRTTEAMPGTPPWARSAEIWAPSVAKIGGRYV
                     MFFAAHRSGVPDSKSDQCVGRALATSPAGPYSPDPVPVTCGTNGGGALDPSVFIGPDG
                     SATLLVAMGGSNVNIWSIPLDGAADPSGPAAAILKREQPWQDWFIENPAMFFDGSTYL
                     LAYSAGHWETGDYLTGIARCASPAGPCTDRPSGPWLSSTNGRAGPGGLEFFVGADGAP
                     RVAFHSYAAGDIGPVGKRSTHIRRFFTDPWPRLP"
     gene            complement(5045..>6004)
                     /locus_tag="PKY13_11740"
     CDS             complement(5045..>6004)
                     /locus_tag="PKY13_11740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT80655.1"
                     /translation="AFVLGIHEGPEHGWTEPLTLIGLIVGVAAGIGFVVWELRQTHPL
                     LNIRVFRNRTLASGSLNLMVVFAIMSALFLVLVQFMQAALGYTALGSSVRLLPMAAVL
                     MPLSSVAPLISRRVGVRAMFVGGISLIALGLGLIAGLTSISGGYLSVLPGLMVLAVGV
                     GLTMTPGTAAITGSLPVEEQGVASALNDTVRELGGAVGIALIGSVLSAGYQSSVSDAV
                     VGLPPQAAEVVKEGIGGAIALGSQLGPQGDAVVAAARNAFVDGLALSMWISMGLAIAA
                     AVFAAVWTPSRAEEFAARDAARLDDDLDLELELESAASPLIAT"
BASE COUNT          965 a         2031 c         2082 g          926 t
ORIGIN      
        1 catgggtgat gggcccccac cggtaccggt cgtactcgga gttcagcgtg agatggacgc
       61 cgatgtcctc gccgtctgac tcggcaactg ctgccctcgc ccagggacac ggcaccatga
      121 gcgttgcgct cgtcgcgatg ccgtcacgta gtgccgagaa gcagccctcg ttggcggcgt
      181 ggctcatgcc gaggtcgtcg caattgatga tgagcagccg gtcgtcggct gcgtagccga
      241 ggcgttccgc gagcgtggtc acgacgtcac cgtagcccct ggcaggatca ccgggtcaca
      301 gggtcgcggg tcgctccgag aagtcgttca agaggtcggc gcggccgacg atcaacgggt
      361 cgagcgtgcc gatgcgctcg acgtccttct cggcgtacgg cacctggtgc agcacgaacc
      421 gcatggcgtt gagccgcgcc cgcttcttgc agtccgactt gatgaccgtc cacggggcca
      481 tcgccgtgtc ggtgtgcagg aacatcgttt ccttggcgag cgtgtagtcg tcccacttgt
      541 cgagggaggc gagatcgacc ggcgacagct tccattgctt gagcggatgc cgctcccgct
      601 cgtggaagcg ccgcagctgc tcctcccggc tcaccgagaa ccagaacttg aggagccgga
      661 cgccgctgcg cgccaggctc tgctcgaacg acggggcctg ctgcaggaac tcccggtact
      721 cctcatcgga gcagaatccc atgacccgtt cgacgccggc ccggttgtac caggagcggt
      781 cgaacagcac gatctccccg gcggtcggca ggtgttggac gtagcgctgg aagtaccact
      841 gaccgcgctc ggcatcggtc ggcttctcga gggcgacgac gcgggcaccg cgagggttga
      901 ggtgctccat gaagcgcttg atcgtcccgc ccttgccggc ggcgtcacgc ccctcgaaca
      961 cgatgacgag tcgctccccc gtgtccttgc accaggcctg gagcttcagg agctcgacct
     1021 gcagctggta cttctcgcgc tcgtacgcct tgcgggacat gaggttccgg tacgggtaga
     1081 cacccgactg ccaaccgtcg gacaacatca cgtcgtcggg gtcgggggga ctcccccaca
     1141 gcggctcgtc ctgcgccagc aacgttccga gctgagtcag ctcatcgggc gactggccct
     1201 cgatcagggc ctgcagccag cgggcgactt cctgcaggtc gtcggcccgg gcgccccgca
     1261 gcaggtcgcg cacggcgttg atcgtcgagc cggcggccga atcggcacct tcctcggctt
     1321 cgaaggcggc gatcgcctcg gcggacaggt caggagcacg atccccagaa tcgacggtgc
     1381 gggtgtcagt catcccggca tgctgacgcg accgctcagc acaatggcag ggtcgaaagt
     1441 cctcacccgt cgggtcgaag cactcatcgc agaccggcga acaggtcgtc ttcgggcagg
     1501 tcggtgtcga tcgtgctgcg ggcgaggatg tagtcgtccc acgggtaggt cgccgccgcg
     1561 atctgcgggg tgaggccgaa ccagaacttc tcgttggggt cgacctgggt ggcgtgtgcg
     1621 agcagcgcct cgtcgcggat gtggaggaac gccccgacgt cgatcttcgt ggtgatccga
     1681 tgatcctggt tcggacggtc gaaccagtcc ttcgggaacg gcgactcgat gccgagttcg
     1741 agcatcttct ggtgcgtggc ctcgatccgg gcccgcgccc acgtcgtgta gtacatcttc
     1801 gacggctgcc acgggtcgcc ggcctcggga aaccagtcgg gatcgcccgc ccggtcgaac
     1861 gcgtgcactg tgatctcgtg ggtccgcaga tggtcgggat gggggtagtg cccctgctcc
     1921 tcgtcgtagg tgaggagcac ctgcgggcgg tcccgccgga tgatccgaac cagccgcagc
     1981 acggcgtcgt cgagcggcgc attggcgaag ttggccgggt cggcgttctc gggcatgtcg
     2041 ggcatgcccg agtccttgta gccgagcatc tcgacgcgct ggtagccgat gatctgcgcc
     2101 gaacgctcga gctccttggc tcgcacctcc ggcaggttct cgaccacctc ggggcggtcc
     2161 atcgccgggt tcaggatcga gccgagctcc ccgccggtgc agcacacgag cgttgcgtga
     2221 gccccttctc cgacgtagcg ggcgacgagc gcggccccct tggacgcctc gtcgtcgggg
     2281 tgggcgtgca ccgagaggag tcggagcgct ggcgcgtcgg tcatgtcaat cgtccaatca
     2341 gcggggggcg ccgcggcagt gggtcaggcg ctgcggagca gatcaagcac tgcggagcgg
     2401 cacgagttcg ggttcgccga gggcgtcgtc gtcgtcgccc tcgagcagct tccatccgaa
     2461 caactcggcg agccgttgtc ttccctcgtg ggggccggag gcgatgacct cgtcccccgc
     2521 ctcgaagctc acgaacgggc gggggcgata gagatactgg ccgcgccgca acagtgcgag
     2581 gacgtggaac ccgggttcga tgtcgagctg catgtcggcc atcgacttgc cgaccacggc
     2641 ggcgccgtcg gcgacgggca cccgcatcac caccacgtcg ctctcaccga gcgccattgc
     2701 gagcaccggg tggaactcga tctcgtcgct gatcagccac accatctggc tcgcctggtc
     2761 gcccaactcc tcggcgacgt gggacaactg cagcagacct cgcagcggcg acgggtcgac
     2821 gttgctcgcg ctcgcacgca atacccacag ctgcaggtgg tccttcatct cgtcgagacg
     2881 attggcgagg tgcacgacct cggcggcgag gccacggtcg cgcagagcga gggccgagta
     2941 ggccaggccg accgatgcct ccgagatgtt cttcatctcg accaacacgt cgacggcgcg
     3001 gtcgaggtcg gtgagcgcac ccggagcggg aaccgaagga ggactccacg tgggcgcggc
     3061 ggccagctcg tgcaggcggg tgatgccggg gggcgaccct cgcaggaaca acacgtcgcc
     3121 ggggagcagg acgaggtcgc cgtcgatctc gctgatccag gagcgcccac ggcggatcgc
     3181 catgatgcgc atgccggtcg ccaccggcag ttcgagcgcc gccaccgggc ggttggccat
     3241 gtggcttccc tcgcggatcc agacccggtg tgagacctcc tccgcgttgg agaggtcgcc
     3301 gatcagctcg ttggggatgc cgaggcggtg ggtgacgatg cgggtgatgt cgatggcagc
     3361 gttgccgatg ccctcgatcg cgccgatgac ctggaggacg gccgccatcg actcggcctc
     3421 cgacgggcgg cggaccgcca ggatgcagac ggcccgcatg tcctgcacca gatcgttgag
     3481 acggctctcg agcgctgcga cttcctcgca catgcccggg tcgttgaagt acacggcggc
     3541 gtacgccagg tcgaccatga gttcggaggt gtccttcgcc tccgccaaca tggccctgag
     3601 acttcggggg cgttcatcca taggcggtcc gaggagcgta accgatctgg ttccgcttcg
     3661 gctcacccat ctggattccg acccggtgac aacaccaccg ccccgacgat gacacccgtt
     3721 cggggagacg agtccggaaa cgacgagtat tcagcggggt gtgggtgctg gccgttacgg
     3781 tgaccggatg ccacgccgtt cttccgtcct gctgctcgcg atcctgctcc tgacaaccgc
     3841 cggctgcatc agggccaagc ccggcgcccg ctgcaacagc accgactggg gcgagaacgg
     3901 cacccacgtc atgcgctgcg tgggcggccg ctggaagatc gtgatgaccc gccaacaggc
     3961 gatcgacgcg ctggtcgcgc tgtcagaggc ggccaagaag ccacccgtgg gcgacgtggt
     4021 acgaccgctc gatctcggcc cggccaatct ccccaacacc gccgacccgt cggtgttcgt
     4081 cgagaacggc gtgagctacg tgttctcgac gtcgacctac cagcacgtgc ccgtggcgac
     4141 gatgtccgac atcgacgcgc cgctcgccat gtaccgcacg accgaggcga tgcccggcac
     4201 gccgccctgg gcgcgctcgg cggagatctg ggcgccgagc gtcgccaaga tcggcggccg
     4261 atacgtcatg ttcttcgcgg cgcaccgctc gggcgttccg gactccaaga gcgaccagtg
     4321 cgtcggccgg gccctcgcga cgagcccggc gggaccgtac tcaccagatc ctgtccccgt
     4381 cacctgtggc accaacgggg gcggagcgct cgatccgtcc gtgttcatcg gccccgacgg
     4441 atcggcgacg ctcctcgtcg ccatgggcgg atcgaacgtc aacatctggt ccattccgct
     4501 cgacggagcg gccgacccat ccggtccggc ggccgcgatc ctcaagcggg aacagccctg
     4561 gcaggactgg ttcatcgaga acccggcgat gttcttcgac gggtcgacgt acctgctcgc
     4621 gtactcggcg ggccactggg agaccggcga ctacctcacc ggtatcgccc gctgcgcctc
     4681 acccgccgga ccgtgcaccg accgcccgag cgggccctgg ctctcgtcga cgaacggacg
     4741 cgccgggccc ggcggcctcg agttcttcgt cggcgccgac ggggcgccgc gggtggcgtt
     4801 ccactcctac gccgccggcg acatcggccc ggtcgggaag cgttcgacac acatccgccg
     4861 gttcttcacc gatccgtggc cgcgcctgcc gtgacatcac gccgtcgcgg tcgcgtccgg
     4921 accgtgaccg aactccaggc ccacccgtga gcatggacac gggtgggcct gaagttcggg
     4981 atcaggatcg gcgacggcga cggcagaacc agattcctcc gaggcgccag aacggggtca
     5041 tgactcaggt cgcgatcagc ggggaggcgg ccgactcgag ctcgagctcg aggtcgagat
     5101 cgtcgtcgag acgggcggcg tcgcgggcgg cgaactcctc ggcacggctc ggcgtccaca
     5161 ccgcggcgaa caccgcagcg gcgatggcga gacccatgct gatccacatg gacagcgcca
     5221 ggccgtcgac gaaggcgttg cgggccgctg ccacgacggc gtcgccctgg gggccgagct
     5281 ggctcccgag cgcaatggcc ccaccgatac cttccttgac gacctcagcg gcctgcggcg
     5341 gcagaccgac gacggcgtcg gacaccgacg actggtagcc ggcgctgagc accgaaccga
     5401 tgagagcgat gccgacggcg ccgcccagct cacggacggt gtcgttgagc gccgaggcca
     5461 ccccttgctc ctccaccggc agcgaaccgg tgatggcggc ggtgcccggt gtcatcgtga
     5521 ggccgacacc gacggcgagg accatcaggc cgggcaggac cgagaggtac ccgccgctga
     5581 tgctcgtgag tccggcgatc aggccgagcc cgagcgcgat cagggaaatg cctccgacga
     5641 acatggcgcg aacgccgacc cgacgagaga tgaggggcgc caccgacgag agcggcatga
     5701 gcaccgcggc catcggaagg aggcggaccg acgatccgag agcggtgtag ccgagcgccg
     5761 cctgcatgaa ctgcacgagc acgaggaaca gcgcactcat gatggcgaac acgaccatga
     5821 ggttgagcga ccccgaggcg agcgtgcggt tgcggaagac ccggatgttg agcagtgggt
     5881 gcgtctggcg cagctcccag accacgaagc cgatgccggc ggcgacgccg acgatgaggc
     5941 cgatgagcgt caacggctcg gtccagccgt gctcgggacc ttcgtggatg ccgaggacga
     6001 aggc
//
LOCUS       DAOSOA010000198         5959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13472_length_5959_cov_6.009146, whole genome shotgun sequence.
ACCESSION   DAOSOA010000198 DAOSOA010000000
VERSION     DAOSOA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13472_length_5959_cov_6.009146"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PKY13_11745"
     CDS             complement(<1..1130)
                     /locus_tag="PKY13_11745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT80656.1"
                     /translation="MSDAATDLTAPAPRPFDADNHYYEALDAFSRHLPAEWGPRTIQW
                     AEIDGRMYHVVGGRVSRAVVNATFDPVAKPGVLRDYFRGNPNGDNPIELLKARDRISP
                     AYRDRDVRLDQLDDHGLDAVWMFPTLGMIYEEQLKHDPEAVAVTFKAFNRWVAEDWGF
                     DYKDRIFAAPYISLADVDAAVTELRYGLDNGARTVVMRPAAPTTRDGQKPPTHPDFDP
                     FWALANEAGITVVVHAGDTGYTSNGYANDGFSSNFKGGIPQPIKFTQLERAIEDFLAS
                     LVCDQLFVRFPNLRVASVENGSGFLRGLFGKLNATANKMAGWFSEHPVDAFKQNIWIN
                     PFWEDDVHELLDLMGPDKILFGSDWPHIEGMPRPLDYLEELEG"
     gene            1157..>1566
                     /locus_tag="PKY13_11750"
     CDS             1157..>1566
                     /locus_tag="PKY13_11750"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT80657.1"
                     /translation="MTPDLVGSALPASMHAYRLTEWQRPPELVEIPVPSPGPGEVLVR
                     VAGNGLCHSDITMMGIPGEIGEAIGWRIPFTLGHEAAGWIAAVGNEVVGVDIGDAVAL
                     MSANSCGVCRWCVAGRDSLCQDNMVGRGYGRDGGL"
     assembly_gap    1567..1666
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1667..2107
                     /locus_tag="PKY13_11755"
     CDS             <1667..2107
                     /locus_tag="PKY13_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT80658.1"
                     /translation="AVDQEASRREHALALGAHHAVEGIDRGTRRVLLDLVGPDGLDGI
                     LDIVGTDETIDCGIRSLARGGVFALVGAAGGKLDQPWFSTLPHEAEIFTFQGSSRSDV
                     EAVVGLAREGRIVVESEQFPLARVADAYERMEAGTLTARAVIIP"
     gene            2212..3834
                     /locus_tag="PKY13_11760"
     CDS             2212..3834
                     /locus_tag="PKY13_11760"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOT80659.1"
                     /translation="MGEMNRRGFLRAGAGGAGALWLGGSGLTVVGCTPSPAYNPPANG
                     TPFTLGVLSGLHSASEVVLWTRLEPSLAPGATAVTWEIATDPGMGTIVESGEVTVDGS
                     ADWTAKVLVGGLAADRSYWYRFAVGGLTSPVGRARTLPSPGSSPDRLRLAFASCQNWG
                     SGWYNAWAGIVAEDVDAVVWLGDYIYESGDLAIGSVRKDPVGSVDDLAGYRAKYQLYR
                     SDPLLQAGHAAHPFVPVWDDHEFENDYNRITIAQNPQRAQDAYRAWFEYMPVWPIDGT
                     RIHRSLSWGRLAEIPLLDTRQYRDRQPNGFQGPDVDSYIGAGDVLVEASSPNRTILGT
                     AQRDWLFERLGSAQADGVRWKLIGNQVMIAPVRPIDLDTPELRRLFPDLPRHNGLFVN
                     MDSWDGYQWERDLLLARLSDERIADVSFLTGDIHTFWQASIRADFDDPTSPIVANEYV
                     GGSISSPGPNVIDNDDFGIFIEEATRNWSPAFRYSDFRRNGYGLLDATPDTLDISFRT
                     TRVKTLGTPVATSVAFGTTPGSPVPSLTAVNP"
     gene            complement(3931..4329)
                     /locus_tag="PKY13_11765"
     CDS             complement(3931..4329)
                     /locus_tag="PKY13_11765"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOT80660.1"
                     /translation="MTPPDATAAVVIAHGSRAEAANDAHRDVCAALAERTGGPVLPAF
                     LELAEPTLDDAVARLVADGATRVVVLPYFLYPGRHIQRDIPALVDGARAANPTVTIEV
                     GQLFGADPAVLDLLATQLSLAAGPDAAEDR"
     gene            4347..4976
                     /locus_tag="PKY13_11770"
     CDS             4347..4976
                     /locus_tag="PKY13_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT80661.1"
                     /translation="MSEPDDPRLDSVGVGPHPEPWPVDPRFDPELLANGDRRNVVDRY
                     RYWTLEAIVADLDARRHPFHVAIENWEHDFNIGTVVRNANAFLAAEVHIVGRRRWNRR
                     GAMVTDRYQHVRHHATIDDLAAWCAERDLPLIGIDNLPDSTPIETTELPEACVLLFGQ
                     EGPGLSDAARAAADRTCSIAQFGSTRSINAGVASGIAMHAWIRRHAMLS"
     gene            4976..5521
                     /locus_tag="PKY13_11775"
     CDS             4976..5521
                     /locus_tag="PKY13_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80662.1"
                     /translation="MRRRVRRLVGSGVALALLGLVGGCADDGRTATTSGPVDTTGAVD
                     GARLIVITMDDYEFRPSALTAKAGETVTLRFINEGTVRHEAVIGDETVQRAHAEMMMS
                     MTTTTVVPRVTDPTTTAPISSLHGRSRAHPGMNAPNVVSVEAGATGEVTYSLAKKAHL
                     LIGCHEPEHYELGMVATLDVV"
     gene            5556..>5959
                     /locus_tag="PKY13_11780"
     CDS             5556..>5959
                     /locus_tag="PKY13_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT80663.1"
                     /translation="MSEVDLGLSHIALPVHDLERSLDFYRRFAGLEVVHRRESHPGHA
                     VAWISDLTRPFVVVLLQTDAPASPGRLGGWAHLGVGCLSTAEVDRRLAEAASEGYEII
                     GPVSDGPPVGYWGIIVDPDGHNLELAYGQQVGL"
BASE COUNT          856 a         2020 c         2045 g          938 t
ORIGIN      
        1 ccctcgagtt cctcgaggta gtcgagcggc cggggcatgc cctcgatgtg gggccagtcg
       61 gatccgaaga ggatcttgtc ggggcccatg aggtcgagca gctcgtggac gtcgtcctcc
      121 cagaacgggt tgatccagat gttctgcttg aacgcgtcga cggggtgctc gctgaaccag
      181 ccggccatct tgttggccgt cgcgttcagt ttgccgaaga ggccacggag gaagccggaa
      241 ccgttctcga ccgacgccac gcgcaggttc gggaagcgca cgaacagctg gtcgcacacg
      301 agcgacgcca ggaagtcctc gatggcccgc tcgagctggg tgaacttgat gggctgagga
      361 atgccgccct tgaagttgga cgagaacccg tcgttcgcgt agccgttgga cgtgtagccc
      421 gtgtcgccgg cgtgcacgac cacggtgatg ccggcctcgt tcgccagcgc ccagaacgga
      481 tcgaaatcgg ggtgggtggg aggcttctgt ccgtcgcggg tcgtcggcgc cgccggacgc
      541 atcacgaccg tgcgggcgcc gttgtcgagg ccgtagcgca gctcggtgac cgcggcgtcg
      601 acgtcggcga ggctgatgta gggcgccgcg aagatgcggt ccttgtagtc gaagccccag
      661 tcctcggcga cccaccggtt gaacgccttg aacgtcacgg cgaccgcttc ggggtcgtgc
      721 ttgagctgct cctcgtagat catgccgagg gtcgggaaca tccagacggc gtcgagcccg
      781 tggtcgtcga gctggtcgag gcggacgtca cggtcgcggt atgccgggga gatgcggtcg
      841 cgggccttga ggagctcgat ggggttgtcg ccgttggggt tgccccggaa gtagtcgcgc
      901 agcacaccgg gcttcgccac cggatcgaac gtggcgttga ccaccgcacg gctgacccgg
      961 ccgccgacga cgtggtacat ccgcccgtcg atctcggccc attggatcgt gcgcgggccc
     1021 cactcggcgg gcaggtgccg actgaaggcg tcgagcgcct cgtagtagtg gttgtcggcg
     1081 tcgaagggcc gaggtgccgg tgcggtgaga tcggtggcgg cgtcgctcat acgtgcagct
     1141 tgctaccgtc cgggccgtga caccagatct tgttggatca gcgcttccgg cctcgatgca
     1201 cgcctatcga ctgaccgagt ggcaacgtcc gcctgagctg gtggagatcc cggtccccag
     1261 ccccggcccc ggcgaggtgc tggttcgggt cgccggcaac ggcctgtgcc attccgacat
     1321 cacgatgatg gggattccgg gcgagatcgg tgaggcgatc ggttggcgga tcccgttcac
     1381 gctcggtcac gaggcggccg ggtggatcgc ggcggtcggc aacgaggtcg tcggcgtcga
     1441 catcggcgac gccgtggcgc tgatgtcggc caactcgtgt ggtgtgtgcc ggtggtgcgt
     1501 ggccggtcgc gacagcctgt gccaggacaa catggtgggg cgtggctacg gacgcgacgg
     1561 gggcctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg tcgaccagga
     1681 ggcctcccgt cgtgagcatg ccctcgcgct cggcgcccat cacgccgtcg agggcatcga
     1741 ccgtggcacc cggcgagtcc tgctcgatct ggtcggtccc gacggactcg acggcatcct
     1801 cgacatcgtc ggcaccgacg agacgatcga ctgcggcatc cggtcgctcg cccgtggtgg
     1861 cgtcttcgcg ctcgtgggcg cggcgggcgg caagctcgac caaccctggt tctccacgct
     1921 gccccacgag gccgagatct tcaccttcca gggatcgagc cgctccgacg tggaggcggt
     1981 cgtcgggctg gcccgtgagg gtcgcatcgt cgtcgagtcc gagcagttcc cgctcgcccg
     2041 ggtcgctgac gcctacgagc gcatggaagc gggcaccctg accgcccgcg ccgtcatcat
     2101 cccctgaccg tcggggttct gtagtgcgag gagcgacgcc agcgtcgctc ctcgcactac
     2161 aaaacccctg gggagctggc gtactctcgg cggagagctg ggaggatctg tatgggcgag
     2221 atgaacaggc gcgggttcct gcgcgctgga gccggtgggg cgggcgcgct ctggctgggc
     2281 gggtccggtc tgacggtcgt cggctgcacg cccagtccgg cgtacaaccc gccggccaac
     2341 ggcaccccgt tcaccctcgg tgtgctgtcg ggtctccaca gcgccagcga ggtcgtgctc
     2401 tggacccggc tcgagccctc gttggccccg ggcgccacgg cggtgacgtg ggagatcgcc
     2461 accgatcccg gcatgggcac gatcgtcgaa tcgggcgaag tgaccgtcga cggatcggcc
     2521 gactggacgg ccaaggtgct ggtcggtggg ctcgccgccg accggtcgta ctggtaccgg
     2581 ttcgccgtcg gcggcctcac gagcccggtc ggccgcgccc gtaccctccc gtcgccgggc
     2641 tcgtcgcccg accgtctgcg tctcgcgttt gcgagctgcc agaactgggg ctcggggtgg
     2701 tacaacgcct gggccggaat cgtcgcggag gacgtcgacg ccgtcgtgtg gctcggcgac
     2761 tacatctacg agtccggcga ccttgccatc ggatcggtcc ggaaggatcc cgtgggatcg
     2821 gtcgacgacc tcgccggcta ccgcgccaag taccagctgt accggtccga ccctctgctc
     2881 caggcgggtc acgccgcgca cccgttcgtc ccggtgtggg acgaccacga gttcgagaac
     2941 gactacaacc gcatcaccat cgcccagaac ccgcagcggg cccaggatgc gtatcgcgcc
     3001 tggttcgagt acatgccggt gtggccgatc gacggcaccc gcatccatcg cagcctgtcg
     3061 tggggccggc tcgccgagat cccgctcctc gacacccgcc agtaccgcga ccgccagccc
     3121 aacgggttcc agggccccga cgtcgactcc tacatcggtg cgggcgacgt gctcgtcgag
     3181 gcgtcctcgc cgaaccgcac gatcctcggc acggcccagc gtgactggct gttcgaacgg
     3241 ttgggttcgg cccaggccga cggtgtgcgt tggaagctca tcggcaacca ggtgatgatc
     3301 gccccggtcc ggccgatcga cctcgacacc cccgaactcc gacggttgtt ccccgatctg
     3361 ccccgtcata acggtctgtt cgtcaacatg gacagctggg acggctacca gtgggaacgc
     3421 gacctgctgc tcgcccgcct ctccgacgaa cgcatcgccg acgtgtcgtt cctcacgggc
     3481 gacatccaca cgttctggca ggcgtcgatc cgggccgatt tcgacgatcc cacgtcgccg
     3541 atcgtcgcca acgagtacgt cggcgggtcg atctcgtcgc cgggccccaa cgtcatcgac
     3601 aacgacgact tcgggatctt catcgaggag gcgacccgca actggagccc ggcgttccgc
     3661 tacagcgact tccggcgcaa cggctacggg ctgctcgacg cgacgccaga cacgctcgac
     3721 atcagcttcc ggaccacccg ggtgaagacg ttgggcacgc cggtggcgac atcggtggcg
     3781 ttcgggacga ccccgggctc gcccgtcccg tcgctgaccg ccgtcaaccc gtagccggat
     3841 cggccacgtt tttctccagt cgattccggt ctgcgtcgaa agatctgttg tcgcggcgag
     3901 gggaagcgcg agggccggtc gaccgtcagg tcaccggtcc tccgccgcgt ccggacccgc
     3961 ggccagcgac agctgcgtgg cgaggaggtc gagcaccgcc ggatcggcac cgaagagctg
     4021 cccgacctcg atggtcacgg tcgggttcgc ggcccgggcc ccgtcgacca gtgcggggat
     4081 gtcgcgctgg atgtgccggc ccgggtagag gaagtacggg agcaccacga cgcgggtggc
     4141 gccgtcggcc acgaggcggg ccacggcatc gtcgagcgtc ggttcggcca gctccaggaa
     4201 cgccggcagg accggaccgc ccgtccgttc ggccaaggcc gcacacacgt cgcgatgggc
     4261 gtcgttcgcg gcctcggccc ggctgccgtg ggcgatcacg acggcggcgg tggcatcggg
     4321 gggcgtcacg cggccaacct tacgacgtgt ccgagcccga cgatccccgc ctcgactccg
     4381 tgggcgtggg cccacacccc gagccctggc cggtcgatcc ccggttcgac cccgagttgt
     4441 tggcgaacgg cgaccggcgc aacgtcgtcg accgctaccg gtactggacg ctcgaggcga
     4501 tcgtcgccga cctcgacgct cgccgtcatc cgttccacgt ggccatcgag aactgggagc
     4561 acgacttcaa catcgggacc gtcgtccgta acgccaacgc gttcctggct gccgaggtgc
     4621 acatcgtcgg gcgtcgccgt tggaaccgcc ggggggccat ggtcaccgac cgctaccagc
     4681 acgtccgaca ccacgccacg atcgacgacc tcgcggcctg gtgtgctgag cgggacctcc
     4741 cgctgatcgg gatcgacaac cttcccgact ccaccccgat cgagaccacg gagctgccgg
     4801 aggcgtgtgt gctcctcttc ggtcaggagg gcccgggcct ctccgacgca gctcgcgctg
     4861 ctgccgatcg cacgtgttcc atcgcccagt tcggctcgac gcgttccatc aacgccgggg
     4921 tcgcgtcggg catcgcgatg cacgcatgga tacggcgaca tgcgatgctc tcgtgatgcg
     4981 gcgtcgggtg cggaggctgg tggggagcgg tgtggcgctg gcgctgctgg gccttgtcgg
     5041 cgggtgcgcc gacgacggcc ggacggccac gacgagcggc cccgttgaca ccacgggagc
     5101 cgtcgacggc gcccggctca tcgtcatcac catggacgac tacgaattcc ggccgtcggc
     5161 cctcaccgcc aaggcgggcg agaccgtcac gctgcggttc atcaacgagg gaacggtccg
     5221 gcacgaggcg gtcatcggcg acgagaccgt gcagcgcgcc cacgccgaga tgatgatgtc
     5281 gatgaccacg accacggtgg tgccgcgggt caccgatccg acgacgacgg ctccgatctc
     5341 gtcgctccac ggtcgctccc gcgcccaccc gggcatgaac gcacccaacg tggtctccgt
     5401 cgaggcgggg gccaccggcg aggtgaccta cagcctggcc aagaaggcgc acctgctcat
     5461 cggatgccac gaacccgagc actacgagct cggcatggtg gcgaccctcg acgtggtctg
     5521 atcggccact gcggctgtga caaggtgacg gacccatgag tgaagtagat ctcggcctct
     5581 cccacatcgc gctccccgtc cacgatctgg aacgcagcct cgacttctac cggcgcttcg
     5641 ccggcctcga ggtcgtgcac cgccgcgagt cgcacccggg ccacgccgtg gcgtggatct
     5701 cggatctgac ccgcccgttc gtcgtcgtcc tgctccagac cgatgccccg gcgtcgccgg
     5761 gccgactcgg cggctgggcc cacctcgggg tcggctgcct gtcgaccgcc gaggtcgacc
     5821 gtcgcctcgc cgaggcggcc agcgaggggt acgagatcat cgggccggtg tcggatgggc
     5881 cgcccgtggg ctattggggc atcatcgtcg accccgacgg tcacaacctc gagctggcgt
     5941 acggccagca ggtcggcct
//
LOCUS       DAOSOA010000199         5935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13546_length_5935_cov_5.392687, whole genome shotgun sequence.
ACCESSION   DAOSOA010000199 DAOSOA010000000
VERSION     DAOSOA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13546_length_5935_cov_5.392687"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PKY13_11785"
     CDS             <1..646
                     /locus_tag="PKY13_11785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT80664.1"
                     /translation="DAARPELKAANHDALCDELGLAAGPGPLVVSVSRFDYQKGIDLL
                     GEAGGILAGIPVRLALLGSGEWDTEAAMARLAEAHPGRIAFRQGYDAALAHRMFAAGD
                     LAIIPSRFEPCGLTQMQAMRYGTVPIVSDVGGLHDTVVDIDAAPRTGTGVLLRSVSAA
                     GIVDGVHRANRLWSNKAKAARVRRRAMEIDWSWDAPAARYRSLYEGLVSGAETA"
     gene            678..1913
                     /locus_tag="PKY13_11790"
     CDS             678..1913
                     /locus_tag="PKY13_11790"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOT80665.1"
                     /translation="MAQPNVLVMVLAGGEGKRLLPLTNDRAKPAVPFGGSYRLIDFVL
                     SNFANAQYTRIVVLTQYKSHSLDLHISRTWRFSTLLGSYVSSVPAQMRLGPYWFSGSA
                     DAIYQNLNLITDEKPDIVCVFGADHIYRMDPRQMVDYHQQHGGGVTVAAIPVPRDQAS
                     AFGIIEANAEGTITRFLEKPEDPPPMHDNPDMCLASMGNYVFDTDVLVEAVTPGSLTG
                     LPHDIGGDLMPRLAERASARVYDFSTNDVPGQGERERGYWRDVGTVDAYYDANMDLVA
                     SVPVFSLYNEDWQVFTYSPPRPPAKLSRGPSGRLPEVTDTLVCSSTILSGSEASGSII
                     GPGSFVGEASLDGTILLPGVHVGQDAVLRRCVVDKNVVIPPGTQIGVDREADIARGLV
                     VSAGGVTVVEKGFQFPEPV"
     gene            1910..5545
                     /gene="glgB"
                     /locus_tag="PKY13_11795"
     CDS             1910..5545
                     /gene="glgB"
                     /locus_tag="PKY13_11795"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOT80666.1"
                     /translation="MTGPEEPAAEATAAAPATAATSELHERARAHGVATSYVDGTGVE
                     RVPSDATLSAILDALDAPGGSNPFHSSTVEAPQPGDRVPERCAHGPQPDRTWGVFAPL
                     AALRRTNAPDHGAGDLTGLAELAQVVEAAGGSVVGTLPLVATRPDEASPYSPLTRRFW
                     DERWVDPEWVATRLGAATPTPRAVVASDLADHEAAWTSTRAALAEVLGGFDGLPEETA
                     TWRSHHPEVEQWARWKAAALVAGWDPTFWTDELDLAVRSSDDAALAALGVSSAMVDFE
                     VFSQWAVTAQLTQLGSDLRARSMSLYLDLPVGTNAAGYDAWVAPDAFGARMSIGAPPD
                     LFFPDGQSWGLRPPLPGTEAGLADLRHSLETHMAVCGLLRIDHVMGLDRLFWVPEDGE
                     AGEGTYVTYDAGERWRLVSELSQHFGCGVVGEDLGTVPQSTRDAMDAVGARGLFIGQD
                     EVRSPFRLGRPVPAEAVATLNTHDLPPMAAWHTERFGAGTDGPAPDSTTVRDHLLGEL
                     AMSNADVVMVADQDLTLDDRRFNVPGQVGGELWRLRSLTSVADLRAGVRGGRDARAVL
                     ANVDAWRSTQRGAWPAGVEPHLDDGDVSSLHAGTHARLADRFGVHPRTAAGVIGCEAA
                     VWAPHASAVAVAGEFDGWSGALLHRRGDSGVWSGFIPSAVLGDRYKLHICGADGRWVH
                     KSDPFARAAELPPGNASIITADEPAEHGWNDAAWMAARHDAQAPGQAMSIYELHLGSW
                     RQGDGGRTLSYAEITPLLVEYVQRMGFTHVELMPVMEHPFGGSWGYHVTGYFAPTDRY
                     GTPADFASMVDALHVAGIGVILDWVPAHFPDDEHGLADFDGQALFEYADPREGRHPEW
                     GSRVFDFGRPEVRAFLVSSARWWIEKYHVDGLRVDAVASMLYRNYARNDGEWVPNVHG
                     GRENLEAVDFVRQLTSEIHHSTPGAIVIAEESTAWPGVTAPSEGGGLEFDLKWDLGWM
                     HDMLEYLGRDPVHRSWHQDDLTFRAMYSTSERFVLPLSHDEVVHGKSSLVGKMAGDDW
                     QRRANLRLLFGHQFTVPGVPLVFMGGELAMNEEWSHERSLPWHLLDYPAHSGVQAWVA
                     ELNRLLRAHPALAVTSSDPGTFEWVDCDDRANSVVSWVRRSPSGEQLLVTANFTPTPH
                     EGYRIGAPAPGTWHEIANSDDVRWGGSGFPTPDRHDSAPIPAKSWHHSLVVTLPPLAI
                     SVFATAP"
     gene            5642..>5935
                     /locus_tag="PKY13_11800"
     CDS             5642..>5935
                     /locus_tag="PKY13_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80667.1"
                     /translation="MTAYVCVHGHAYQPPRESPDTGEVERQPSAEPFHDWNERVTSEC
                     YAPLSQAKRLDEQGSTLSVTNLWEHLSFDLGPTLGVWMDRRVPGVATSIAVGDS"
BASE COUNT          850 a         2109 c         2043 g          933 t
ORIGIN      
        1 cgatgccgcc cgccccgagc tcaaggcggc caaccacgat gcactttgcg acgagctcgg
       61 tctggcggcc ggtcccggcc cgctcgtggt gagcgtgagc cgattcgact accagaaggg
      121 catcgacctg ttgggcgagg ccggcggcat cctcgccggc atcccggttc gcctcgcgct
      181 cctgggttcg ggggagtggg acaccgaagc ggcgatggcg cggctcgccg aggcgcaccc
      241 cggtcggatc gcgttccggc agggctacga cgccgcgctg gcccaccgga tgttcgccgc
      301 cggtgacctc gccatcattc cgagccggtt cgagccgtgc gggctcaccc agatgcaggc
      361 gatgcgctac ggcacggtac cgatcgtgag cgacgtcggg gggctccacg acaccgtcgt
      421 cgacatcgat gctgctcccc ggaccggcac cggggtgctc ctccgttcgg tgtcggcggc
      481 cggcatcgtc gacggggtgc accgcgccaa ccggctgtgg tcgaacaagg cgaaggcggc
      541 ccgggtgcgc cgccgggcca tggagatcga ctggtcctgg gacgctcccg cggcgcggta
      601 ccggtcgctg tatgaaggct tggtttcggg ggccgaaacg gcctgaacgg ccctgatgcg
      661 ccgataggct cgcgggcgtg gcacaaccca acgtcctcgt catggtcctc gcgggcggtg
      721 agggcaagcg tctccttccc ctcaccaacg atcgagccaa gcccgccgtc ccgttcggcg
      781 gcagctaccg cctgatcgac ttcgtgctgt cgaacttcgc gaacgcgcag tacacccgca
      841 tcgtggtgct cacgcagtac aagagtcaca gcctcgacct ccacatcagc cggacgtggc
      901 ggttctcgac actgctcggg agctacgtga gctcggtgcc ggcgcagatg cgcctcgggc
      961 cctactggtt cagcggttcg gccgacgcga tctaccagaa cctcaacctg atcaccgacg
     1021 agaagcccga catcgtctgc gtgttcggtg ccgaccacat ctaccgcatg gatccccggc
     1081 agatggtcga ctaccaccag cagcacggag gtggcgtcac cgtcgccgcg atcccggtgc
     1141 cacgcgacca ggcgtcggcg ttcggcatca tcgaggcgaa cgcggaaggc acgatcaccc
     1201 gcttcctcga gaagcccgag gatccgccgc cgatgcacga caaccccgac atgtgcctgg
     1261 cgtcgatggg taactacgtc ttcgacaccg acgtgctcgt cgaggcggtg acccccggca
     1321 gcctcaccgg tctgccccac gacatcggcg gcgatctcat gccgcggctg gccgagcggg
     1381 cgagtgcccg ggtgtacgac ttcagcacca acgacgttcc aggtcagggt gagcgcgagc
     1441 gtgggtactg gcgtgacgtg ggaacggtcg acgcgtacta cgacgccaac atggacctcg
     1501 tggcctccgt gcccgtgttc agcctctaca acgaggactg gcaggtgttc acctattcgc
     1561 cgccgcgacc gccggccaag ctgtcgcgcg gcccgagcgg tcggctgccc gaggtgaccg
     1621 acacgctggt gtgctcgtcg acgatcctgt cgggctcgga ggcgtcgggg tcgatcatcg
     1681 ggccgggctc gttcgtcggc gaggcctcgc tcgacggcac gatcctgttg ccgggtgtgc
     1741 acgtcggcca ggacgcggtg ttgcggcgtt gtgtggtcga caagaacgtc gtgatcccgc
     1801 cgggcacgca gatcggtgtc gaccgtgagg ccgacatcgc ccgcggtctg gtggtgtccg
     1861 ccggcggggt gacggtcgtc gaaaaggggt tccagttccc cgaacccgta tgacgggccc
     1921 cgaggaacct gccgccgagg ccacggccgc cgcgcccgcc acggccgcca cgtccgaact
     1981 gcacgagcgg gcgcgggccc atggtgtcgc gacgtcgtac gtcgacggca ccggtgtcga
     2041 gcgggttccg tccgacgcga ccctcagcgc catcctcgac gcgctcgacg cgcccggcgg
     2101 ctccaacccc ttccacagtt caactgtgga ggcaccgcag ccgggggacc gggtgccgga
     2161 acggtgcgcc cacggccccc aaccggaccg gacatggggg gtgttcgcgc cgctcgccgc
     2221 acttcgccgg accaacgcac ccgatcacgg tgccggtgat ctcaccggcc tggccgagct
     2281 cgcgcaggtc gtcgaggcag cgggtggctc ggtcgtgggg acccttccgc tcgtggcgac
     2341 ccggcccgac gaggccagcc cgtactcgcc gctgacgcga cggttctggg acgaacggtg
     2401 ggtcgatccc gagtgggtcg ccacccgcct gggcgccgcg acgccgacgc cgcgtgcggt
     2461 ggttgcgtcc gatctcgctg accacgaggc ggcttggacg tcgacgcgcg ctgcacttgc
     2521 cgaggtgctc ggcggattcg acggacttcc cgaggagacg gcgacctggc gctcgcatca
     2581 tcccgaggtc gagcagtggg cccgttggaa ggccgcagcg ctcgtcgccg ggtgggatcc
     2641 caccttctgg acggacgagc tcgaccttgc ggtgcgctcg tcggacgacg cggcgctcgc
     2701 agcgctcggc gtttcgtcgg ccatggtcga cttcgaggtg ttctcccagt gggcggtgac
     2761 cgcacagctc acccagctgg gctccgacct tcgtgcccgt tccatgtcgc tctacctcga
     2821 cctgccggtc ggcacgaatg cagccggcta cgacgcctgg gtcgcccccg atgcgttcgg
     2881 cgcccgcatg tcgatcgggg cgccgcccga cctcttcttc cccgacggcc agtcgtgggg
     2941 gctgcggcca ccgctgcccg gaaccgaggc cgggctcgcc gacctccgcc acagcctcga
     3001 gacgcacatg gccgtgtgtg gcctgctgcg catcgaccat gtgatgggcc tcgaccgact
     3061 cttctgggtg cccgaagatg gcgaggccgg cgagggtacc tacgtcacct acgacgccgg
     3121 cgagcgctgg cggctcgtct ccgagttgtc ccagcacttc gggtgcggcg tcgtcggcga
     3181 ggatctcggg acggtcccgc agtcgacgcg cgacgcgatg gacgccgtgg gggcacgggg
     3241 cctgttcatc ggacaggacg aggtgcgatc acccttccgg ctcgggcggc ccgttccggc
     3301 cgaggccgtc gccaccctca acacccacga cctcccgccg atggcggcgt ggcataccga
     3361 gcggttcggg gccggaaccg acgggcccgc acccgactcg accaccgtcc gcgaccacct
     3421 gctcggcgag ctggccatga gcaacgccga cgtcgtcatg gtggccgacc aggacctgac
     3481 cctcgacgac cggcgcttca acgtgcccgg tcaggtgggc ggcgagctct ggcggctccg
     3541 ctccctcacg tcggtcgccg atctccgcgc cggcgtgcgt gggggacgcg acgcccgtgc
     3601 ggtgttggcg aacgtcgatg cctggcgctc gacgcagcgc ggtgcgtggc cggccggcgt
     3661 cgagccccac ctggacgacg gcgacgtgtc gtccctccac gccggtaccc acgcgcgact
     3721 cgccgaccga ttcggcgtcc acccgcgaac ggccgccggc gtgatcggct gcgaggcggc
     3781 cgtgtgggca ccccacgcgt cggccgtggc cgtggcggga gagttcgacg gctggtccgg
     3841 cgccctcctg caccgccggg gtgacagcgg agtgtggtcc ggattcatcc catcggcggt
     3901 gctcggcgac cgctacaagc tccacatctg cggcgccgac ggccgctggg tccacaagtc
     3961 cgatccgttc gcccgcgccg cggaactccc gccgggcaac gcctcgatca tcacggccga
     4021 cgaacccgcc gagcacggct ggaacgacgc ggcctggatg gcggcgcgcc acgacgctca
     4081 ggccccgggt caggcgatgt cgatctacga gctccacctc ggatcttggc gccagggcga
     4141 tggcggtcgc accctctcct acgccgagat caccccgctg ctcgtcgagt acgtgcagcg
     4201 gatggggttc acccatgtcg aactgatgcc ggtgatggag cacccgttcg gcggctcgtg
     4261 gggctaccac gtgaccggct acttcgctcc gaccgaccgc tacggaacgc cggcggactt
     4321 cgcgtcgatg gtcgatgccc tccacgtcgc cggcatcggg gtgatcctcg actgggtgcc
     4381 ggcgcacttc cccgacgacg agcacggcct cgccgacttc gacggtcagg ccctgttcga
     4441 gtacgccgat ccgcgtgaag gtcggcatcc ggagtggggg agccgggtgt tcgacttcgg
     4501 acgtccggag gtgcgggcct tcctcgtgtc gtcggcgcgc tggtggatcg agaagtacca
     4561 cgtcgacggg ctccgggtgg atgccgtggc atcgatgctc tatcgcaact acgcccgcaa
     4621 cgacggcgag tgggtcccga acgtgcacgg cggccgtgag aacctcgagg ccgtcgactt
     4681 cgtacgccag ctgaccagtg agatccacca ttcgacgccg ggcgcgatcg tcatcgccga
     4741 ggagtcgacc gcatggccgg gagtgaccgc cccgtccgag ggcggcggcc tcgagttcga
     4801 cctcaagtgg gacctcggct ggatgcacga catgttggag tacctggggc gcgatccggt
     4861 gcacaggtcg tggcaccagg acgacctcac gttccgggcc atgtactcga cgtcggagcg
     4921 gttcgtgctg cccctcagcc acgacgaggt cgtgcacggc aagtcctccc tggtcgggaa
     4981 gatggccggc gacgattggc agcggcgcgc caaccttcgc ctcctgttcg ggcaccagtt
     5041 caccgtgccc ggcgttccgc tggtgttcat gggcggggag ctggcgatga acgaggagtg
     5101 gagccacgag cgttctctcc cgtggcacct gctcgactac cccgcccatt ccggcgtgca
     5161 ggcgtgggtg gccgagttga accgtctgct tcgagcccac ccggcgctcg cggtcacgag
     5221 ttccgacccc ggaacgttcg agtgggtcga ctgcgacgac cgggccaaca gcgtggtgtc
     5281 gtgggtacgg cgttcgccgt cgggcgagca gttgctcgtg accgccaact tcaccccgac
     5341 gccccacgag ggataccgga tcggtgcgcc cgctccgggc acctggcacg agatcgcgaa
     5401 cagcgacgac gtccgctggg gaggatcggg attcccgact ccggatcgcc acgattccgc
     5461 gccgattccg gcgaagtcct ggcaccattc tctggtggtc acgctgcctc cgctggcgat
     5521 ttccgtcttc gccacggccc cgtgacatcg cccgccccac ctcgatctgc cgttcccggc
     5581 accttcccga cctgattccc ccaaactgat tccccgatct gatctttcaa ggagttcccg
     5641 agtgaccgcc tacgtgtgcg tgcatggcca cgcctaccag ccaccccgcg aatcgccgga
     5701 cacgggcgag gtggaacggc aaccgtcggc cgaaccgttc cacgactgga acgagcgtgt
     5761 gacgtcggag tgctacgcgc cgttgtcgca ggccaagcgc ctcgacgagc agggttcgac
     5821 gttgtcggtg accaacctgt gggagcacct cagcttcgac ctcgggccga ccctcggagt
     5881 ctggatggac cggcgtgtcc ccggcgtggc gaccagcatc gcggtcggtg acagc
//
LOCUS       DAOSOA010000200         5933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13550_length_5933_cov_6.564648, whole genome shotgun sequence.
ACCESSION   DAOSOA010000200 DAOSOA010000000
VERSION     DAOSOA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13550_length_5933_cov_6.564648"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /gene="rpsA"
                     /locus_tag="PKY13_11805"
     CDS             <1..365
                     /gene="rpsA"
                     /locus_tag="PKY13_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231034.1"
                     /note="in Escherichia coli this protein is involved in
                     binding to the leader sequence of mRNAs and is itself
                     bound to the 30S subunit; autoregulates expression via a
                     C-terminal domain; in most gram negative organisms this
                     protein is composed of 6 repeats of the S1 domain while in
                     gram positive there are 4 repeats; the S1 nucleic
                     acid-binding domain is found associated with other
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOT80668.1"
                     /translation="PGEELWVKIIDLDLQRRRISLSIKQAAEGGIVAAEYQEHFGEHA
                     YDDEGNYIGSSLDPDATSEGQEAWEQYYAEQGFQADGVTPIGETPAPVAEAADDAAGD
                     VADEAPVEAAAEGEPEGE"
     gene            652..1542
                     /locus_tag="PKY13_11810"
     CDS             652..1542
                     /locus_tag="PKY13_11810"
                     /inference="COORDINATES: protein motif:HMM:NF028286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HOT80669.1"
                     /translation="MSSRRTLILIGALVTGLISALLIYKYVGGIEEKAQGDAQLVNVV
                     IAKDTIRKGENATDLIARQVIAVGERRQIDLPADAITRVDEVKSQIAQLDIAPGTVIT
                     GAMFDSDAAISETVSNALRDGMVATTVSVDQVHAVAGLVATGDYVNLTVVGQCRLGSA
                     GKLEIDGGSVSGGTGGEGGDAAAAPVETVGCAASLYQKARILGIGRSLGTAASTPVAT
                     GDPAAAAATTVAPTSDMITFELPPEAAQRLQLAGSVYMSLVRKDYKPKPIAIDPFLLV
                     EGVDGATPYGGDPETQLDGQ"
     gene            1542..2969
                     /locus_tag="PKY13_11815"
     CDS             1542..2969
                     /locus_tag="PKY13_11815"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOT80670.1"
                     /translation="MSNYIDQPADADPYAAGSFGNYNPSDGYQPSDSYMASEGYGVPV
                     SGDAYGSAHDPYAANDPYAAPAAHDPYAEPVAQQQPAAIRVSVAVVEPHPPTRDRMLR
                     LLGNGGTPFNSIEELASRLTGSVPVVVILGPSCATAADLGIAERLIQAHSALGAILVV
                     EELTTSLLQQALRAGVKDVLTLSGDATQLSTAIQRVAVTLDSTPRRAASGGDVPMDQG
                     PDVGGPEGGGQLGQVLTVFSTKGGAGKSVIATNLAVALAQRSNQPVVLVDADLQFGDV
                     AVMLKLTPHHTIVDAVAAIDRLDAPLLESLLVTHEMSGLRVLPAPLEPAFADQVGAAE
                     MVAIVELLRTFCSFVIVDTPAYFNDVVLGLVEISDKVVLVAGMDIPNIKNVKIGLQTL
                     RLLNTPMEKLMLVLNRSNSKVKLDIGEVERTLQVKADALIPSDVLIPQSVNKGVPVVL
                     HAPKSPVAKSIQQMTELFLPATKRR"
     gene            3184..4593
                     /locus_tag="PKY13_11820"
     CDS             3184..4593
                     /locus_tag="PKY13_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOT80671.1"
                     /translation="MSLYQRLHEVNQNGPGAVPGAAPAGGGNLGGGGGVARRDPIMDE
                     LRQRIHAHLIDELGPILYDNRLSGDDLRRRVHEQLLSILGQERAPLSAADKAQLIQEV
                     SDDILGYGPIDKLLSDPNVTEVMVNGPEMVFVERSGKIERDATSFVDSDHLRRVIDKI
                     VSAIGRRVDESTPMVDARLPDGSRVNAVIPPLAIGGPFLTIRKFSKDPLQVDDLIRFG
                     SLNAHAARFLQACVVGKLNVIVSGGTGSGKTTLLNVLSSFIPSDERIVTIEDAKELQL
                     MQEHVLSMESRPPNVEGRGEVSIRDLVKNSLRMRPDRIVIGECRSGEALDMLQAMNTG
                     HDGSLTTLHANTPRDALARLETLVLMSGFDLPVRAIREQASGAVDMIVQLSRLRDGSR
                     RVTHITEVQGMEGDVITLQDVFLFDFSMGVDEHGKFRGHLKATGVRPKFTEKLADQGI
                     RLGPEVFASTDMGRRAVGR"
     gene            4590..>5933
                     /locus_tag="PKY13_11825"
     CDS             4590..>5933
                     /locus_tag="PKY13_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT80672.1"
                     /translation="MTGMRRRSGRSRIGSGLALGGALLLTVLTVNVGISGAAVENSTD
                     PLQIRAVDNRNGKLVVDYMYTGKGDASAAQLDVNGTPATATAEPLAAAGLPSSVVVVL
                     DNALSVGNATVQLSKEDLTALTPGVGPISELGLVTTGDGARVAAQPATSFDAVATSFD
                     GIVPRGNSALWDGMVMAARLLEKTGETQRNVVLMVSSVDSASTATYSEMIRALRAANA
                     SVHVVAMTGSGADGAALHELVDRLGGSFQVGTDSEFTKLYGNVADVVEGQYRVSLDDV
                     PAFEGELASLSLQVGDQTVKASYRPNVLSIGADAVRPAPVVANESLGLFGSDLVKYGI
                     VGLVAVGAFGAFFAVANLVTKRRDGLKYALRHYDEGYGGVGSEDDDDQSMAASALLQR
                     AVEVTGKVAEERGFLVRVEALLERADLPLRPAEALTFYLGGAAASVVIAFVLTGNP"
BASE COUNT          940 a         2090 c         1939 g          964 t
ORIGIN      
        1 cgccgggcga ggagctgtgg gtcaagatca tcgatctcga cctccagcgt cgccgcatca
       61 gcctgtcgat caagcaggcc gccgagggtg gcatcgtggc cgccgagtac caggagcact
      121 tcggcgagca cgcctacgac gacgagggca actacatcgg ttccagcctc gaccccgacg
      181 ccacgtcgga aggccaggag gcatgggagc agtactacgc cgagcagggg ttccaggccg
      241 acggcgtcac gccgatcggt gagaccccgg cccccgtggc cgaagctgcc gacgatgccg
      301 ccggcgacgt cgccgacgag gcgcccgtcg aggctgcggc cgaaggcgag cccgaggggg
      361 agtgatcccc acacggcacg tcgatccgct gtcgatcgca caccacgccc tgcttcggca
      421 gggcgtggtg ccgttttcgg ccgacgatcg gtgccatgcc acgacgacca ccggcccgcg
      481 ccatcgctga gcgcgctttc aggcctgggc cgaaatcctc cccaaaaccc taagtagttc
      541 ggtacctcct gccgacgaac aacgtggcca gcgaaatccg tgttggcatc gcagtgcgat
      601 gtcgcccggg tgctgccaac cactcactca gtttcaggga tcgaggtcgt cgtgagttct
      661 cggagaacac tcattctcat cggtgcactg gtcacgggcc tcatctcggc cttgttgatc
      721 tacaagtacg tcggagggat cgaggagaag gcacaaggtg acgcccagtt ggtgaacgtc
      781 gtcatcgcca aggacacgat caggaagggc gagaacgcca ccgatctcat cgcccgtcag
      841 gtgatcgccg tcggcgaacg ccgacagatc gaccttccgg ccgatgcgat cacccgtgtg
      901 gacgaggtga agagccagat cgctcagctc gacatcgctc ccggcacggt gatcaccggc
      961 gccatgttcg attccgacgc cgccatctcc gagacggtca gcaacgccct ccgcgacggc
     1021 atggtcgcca cgaccgtgtc ggtcgatcag gtgcacgccg tcgccggcct cgtcgccacg
     1081 ggcgactacg tcaacctcac cgtggtcggc cagtgccggc tcggttcggc gggcaagctc
     1141 gagatcgacg gtggcagtgt ctcgggcggc accggcggtg agggtggcga cgctgctgcc
     1201 gcaccggtcg agaccgtcgg ctgcgccgca tcgctctacc agaaggcccg catcctcggc
     1261 atcggtcgtt cgctcggcac cgccgcctcg acgccggtcg ccaccggtga tcccgccgca
     1321 gcagcggcga ccacggtggc cccgacgtcg gacatgatca cgttcgagct gccgccggag
     1381 gccgctcagc gcctccagct cgccggcagc gtctacatgt cgctcgtccg caaggactac
     1441 aagccgaagc ccatcgccat cgacccgttc ctcctcgtcg agggtgtcga cggtgccacg
     1501 ccctacggcg gcgaccccga aacccagttg gatggacagt gatgtcgaac tacatcgatc
     1561 agccggctga cgccgatccc tatgccgccg gttcgttcgg gaactacaac ccgtcggacg
     1621 gttaccagcc ctccgacagc tacatggcct ccgagggcta cggtgtcccc gtgagtggag
     1681 acgcctacgg gtcggcccac gacccctacg cagcgaacga tccgtacgcc gcgcccgcgg
     1741 cgcacgatcc ctacgccgag cccgtcgccc agcagcagcc cgctgcgatc cgggtatcgg
     1801 tcgccgtcgt cgagccgcac ccgccgaccc gggaccgcat gctgcgcctc ctcggcaatg
     1861 gtggcacgcc gttcaactcg atcgaggagc tggcgagccg gctcaccggt tcggtgcccg
     1921 ttgtggtgat cctgggcccg tcgtgcgcga cggctgctga tctcggcatc gccgaacgcc
     1981 tcatccaggc ccattcggcg ctcggtgcca tcctcgtcgt cgaggagctc accacctcgc
     2041 tgctgcagca ggcgctgcgt gccggcgtga aggacgtgct gacgctgagt ggcgatgcga
     2101 cgcagctgag caccgcgatc cagcgggtcg ccgtgaccct cgattcgacg ccgcggcgcg
     2161 cggcgtcggg cggtgacgtc cccatggacc agggccccga tgtcggcggt cccgagggcg
     2221 gtggccagct cggccaggtc ctcaccgtgt tctcgaccaa gggcggcgcc ggcaagtcgg
     2281 tcatcgccac gaacctcgcc gtcgcgctcg ctcagcgatc gaaccagccc gtcgtcctcg
     2341 tcgatgccga tctgcagttc ggcgacgtcg ccgtgatgct caagctgacg ccgcaccaca
     2401 cgatcgtcga cgcggtcgcc gcgatcgatc ggctcgacgc tccgctgctc gagtcgctcc
     2461 tcgtgacgca cgagatgtcg ggtcttcggg tgctgcccgc tccgctcgag ccggcgttcg
     2521 ccgaccaggt cggcgctgcc gagatggtcg ccatcgtcga gctcctccgc acgttctgct
     2581 cgttcgtgat cgtggacact ccggcgtact tcaacgacgt cgtgttgggg ctcgtcgaga
     2641 tctccgacaa ggtcgtcctc gtcgccggca tggacatccc gaacatcaag aacgtgaaga
     2701 tcgggctcca gacgctgcgt ctcctcaaca ctccgatgga gaagttgatg ctcgtgctca
     2761 accgctccaa ctcgaaggtc aagctcgaca tcggtgaggt cgagcgcacc ctgcaggtca
     2821 aggccgatgc cctcatcccc tccgacgtgt tgatccctca gtcggtgaac aagggcgttc
     2881 cggtggtgct gcatgcaccg aagtcgccgg ttgccaagtc gatccagcag atgaccgagc
     2941 tgttcctccc ggcgacgaag cgccgctgac ccgatctcgg cggtgcggtc ggtttcgacc
     3001 ggctgcacgt cggaccgtcg gaccgtccag ggcgacccgg ccgcagcgcg gccggcgccg
     3061 ctgcatcccc gatcatctcg ccggtgcgtc cggagcggct gaaccacacc aatcagctca
     3121 gtttcgcggc gatctggccg aagagatcaa cgtcacacct ttcatcgatc aggagcgccc
     3181 cacgtgtccc tctaccaacg acttcacgag gtcaaccaga acggccccgg cgcggtcccc
     3241 ggcgcagcgc cggccggtgg cggcaatctt ggtggtggcg gtggggtggc ccgtcgtgac
     3301 ccgatcatgg acgagttgcg tcagcgcatc cacgcccatc tgatcgacga actcggcccg
     3361 atcctctacg acaaccggct gtcgggcgat gacctgcgcc gccgggtgca cgagcagctc
     3421 ctgtcgatcc tcggacagga gcgggcgccc ctgtcggcgg ccgacaaggc ccagctgatc
     3481 caggaggtgt ccgacgacat cttgggctac ggccccatcg acaagctgct gagcgatccg
     3541 aacgtcaccg aggtcatggt caacggcccc gagatggtgt tcgtcgagcg aagcggcaag
     3601 atcgaacgcg acgcgacgag cttcgtcgac tccgatcacc ttcgccgggt catcgacaag
     3661 atcgtgtcgg ccatcggccg gcgtgtggat gagtccacgc cgatggtgga cgcccgcctt
     3721 cccgacggct cccgtgtcaa tgcggtcatc ccgccgctcg ccatcggcgg accgttcctc
     3781 accatccgaa agttctcgaa ggacccgctg caggtcgacg acctcatccg gttcggttcg
     3841 ctcaacgccc acgcggcccg cttcctccag gcgtgcgtcg tcggcaagct caacgtgatc
     3901 gtgtccggcg gtacgggttc cggtaagacg acgctgctca acgtgttgag ctcgttcatc
     3961 ccctccgacg agcgcatcgt cacgatcgag gacgccaagg agctccagct gatgcaggag
     4021 cacgtgctgt cgatggagtc ccggccgccg aacgtcgagg gtcgcggcga ggtctcgatc
     4081 cgtgatctcg tcaagaactc gctgcgtatg cgtcccgacc gcatcgtcat cggcgagtgt
     4141 cgttccggcg aggccctcga catgctccag gcgatgaaca cgggccacga cggctcgctg
     4201 accacgctcc acgccaacac gccccgtgac gccctcgccc gtctggagac cctcgtcctc
     4261 atgtcgggct tcgacctgcc ggtccgggcc atccgtgagc aggcatcggg cgccgtcgac
     4321 atgatcgtgc agctctcccg tctccgtgac ggctcccgtc gtgtcaccca catcaccgag
     4381 gtgcagggca tggagggcga cgtgatcacc ctccaggacg tgttcctgtt cgacttctcg
     4441 atgggggtcg acgagcacgg caagttccgc ggtcacttga aggcgaccgg cgtccggccg
     4501 aagttcaccg agaagctggc cgaccagggc atccgcctcg gccccgaggt gttcgcgtcg
     4561 accgacatgg gccggcgagc agtcggccga tgaccggcat gcgacggagg tcggggcgga
     4621 gccggatcgg gtcgggcctg gcgctcggcg gcgcgctcct gctcacggtg ctgacggtca
     4681 acgtcggcat cagcggcgcg gcggtcgaga actcgaccga tccgctccag atccgggcgg
     4741 tggacaaccg caacggcaag ttggtcgtcg actacatgta caccggcaag ggtgacgcct
     4801 cggcggcaca gctcgacgtc aacggcacgc ccgccacggc gacggccgaa cccctcgccg
     4861 ccgccggtct cccgtcgagc gtcgtcgtcg tcctcgacaa cgcgctgtcc gtcggcaacg
     4921 ccacggtgca gctgtcgaag gaggacctca ccgcgctgac gccgggggtc ggtccgatct
     4981 ccgagctcgg actcgtcacc accggtgacg gtgcccgtgt cgccgcccag cccgccacct
     5041 cgttcgatgc tgtcgcgacg tcgttcgacg gcatcgtgcc gcggggcaac tccgccttgt
     5101 gggacggcat ggtgatggcg gcgcgcctgc tcgagaagac gggcgagacc cagcgcaacg
     5161 tggtgctgat ggtgtcgtcg gtcgacagcg ccagcacggc cacctactcc gagatgatcc
     5221 gggccctgcg ggcggccaac gcctcggtcc atgtcgtggc catgaccggc tccggtgccg
     5281 acggtgcggc gttgcacgaa ctggtcgacc gcttgggcgg ttcgttccag gtcgggaccg
     5341 acagcgagtt cacgaagctc tacggcaacg tcgccgacgt ggtcgagggc cagtaccggg
     5401 tgtcgctcga tgacgtcccc gccttcgagg gtgagctggc gagcctgtcg ctgcaggtcg
     5461 gcgaccagac ggtcaaggcg agctaccgac ccaacgtgtt gtcgatcggt gccgacgccg
     5521 tgcgtcccgc tccggtcgtc gccaacgagt cgctcggcct gttcggcagc gacctcgtga
     5581 agtacggcat cgtcggtctc gtggccgtcg gagcgttcgg cgcgttcttc gccgtcgcca
     5641 acctcgtcac caagcgtcgt gacgggctca agtacgccct ccgccactac gacgagggct
     5701 acggcggcgt cggcagcgag gacgacgacg accagagcat ggcggcgtcc gcgttgctgc
     5761 agcgtgctgt cgaggtcacc ggcaaggtgg ccgaggagcg gggcttcctg gtgcgggtcg
     5821 aggcgctgct cgagcgtgcc gacctgccgc tccgccccgc cgaagcgctc acgttctacc
     5881 tcggtggcgc cgcggcctcg gtcgtgatcg cgttcgtcct gacgggtaac ccg
//
LOCUS       DAOSOA010000201         5930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13557_length_5930_cov_5.117277, whole genome shotgun sequence.
ACCESSION   DAOSOA010000201 DAOSOA010000000
VERSION     DAOSOA010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13557_length_5930_cov_5.117277"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKY13_11830"
     CDS             <1..393
                     /locus_tag="PKY13_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HOT80673.1"
                     /translation="RIESIKDGVEVVGNRTRVKVVKNKVAPPFRQAEFDIMYGQGISR
                     EGSLLDIGVEMGFVKKSGAWYTYEGEQLGQGRENAKSFLKDNPEIMVEVSEKVRANMG
                     IGDLPEGDADDQPIDLSLNDDVPITLDD"
     gene            710..2167
                     /locus_tag="PKY13_11835"
     CDS             710..2167
                     /locus_tag="PKY13_11835"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOT80674.1"
                     /translation="MSKRYLIRTHGCQMNEHDSERIAGLFEADGMVPADGIDDADVVV
                     LNTCCIRENADNKLYGTLGHLKSVKAARPELEIVVGGCLAQKDRDLIQEKAGHVDVVF
                     GTHNVHRAVDLLEQSRREGPVTEILEAAVLDDRDLFPSALPARREVGYLAWVTIQIGC
                     DNSCAFCIVPSVRGDEISKPFGSIVDEVRQLAADGVTEVTLLGQNVNSYGRDLTLKLR
                     NEPGELDRSAVAGVTWDADGRARSLFADLLAEVGAVEGIRRVRYTSPHPKDFRDDTIA
                     AMADTAAVCEHLHLPLQSGSDTILAAMHRGYTAERYLQKVAAARAGIADLAVTTDIII
                     GFPGETESDFEATLAVAAEVGYDSAYTFVYSPRPGTEAAELTDRFVERSVTVERMNRL
                     RDVVERSSVQRNAARVGRVEEVIVEGPSKKNPGVLTGRTRQNKLCHFAAPQPIRAGSY
                     ALVEVTGSALSHLTGEFREVTHVAQHRTRIPVAAI"
     gene            2173..2721
                     /locus_tag="PKY13_11840"
     CDS             2173..2721
                     /locus_tag="PKY13_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80675.1"
                     /translation="MGVFDSPRDGTPADSPTARLRPDDLVVALARFLHRVHTSAPVDG
                     AEALSATPSDYLALAAERVESGAVTSDSFDEPYRPHAPERLLSIATDLADGLGAVASV
                     PVHGSLALADLRIDGGSVVGWTVPATPRHGDSYVDLSFLARDLAAAIGPAAVPALFDA
                     YGAERPDPVRIEFWVTLRQLLS"
     gene            2799..3743
                     /gene="miaA"
                     /locus_tag="PKY13_11845"
     CDS             2799..3743
                     /gene="miaA"
                     /locus_tag="PKY13_11845"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT80676.1"
                     /translation="MTVCPRSIVIVGPTASGKSALALELARLLGDSEIVSVDSMQVYR
                     RMDIGTGKVTTDERAETPHRLLDLVEPSTEFSVSWFQSEARAVLADLAERGRRAILVG
                     GTGLYHRAVVDDLEIPGEYPELRSELEHEAAAGPDAVAALHRRLAVLDPTAASRCEPT
                     NARRVVRALEVTLGSGRPFSSYGPGLEEYPPSDHLMVGLQVERSTMLPLIEHRVHTMM
                     AAGFVDEVRALAAEEPPLSRTARQALGYRELLSHLAGRSTEVEAVAETVVRTRQFAVR
                     QDRWFRRDPRIEWLPTTCDGPAPTRSLASFIAGSVDST"
     gene            3740..4573
                     /gene="dapF"
                     /locus_tag="PKY13_11850"
     CDS             3740..4573
                     /gene="dapF"
                     /locus_tag="PKY13_11850"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOT80677.1"
                     /translation="MTASLRLTKAHGLGNDFLVALETANPVLTAASVAAGGSLAATAL
                     CDRHRGVGADGLIFGLAPDGSGADLRMVLFNADGSEAEISGNGIRCLGQAALRAGGRR
                     DGRIVIDSGAGRRTLDATATTAPDTDLLRVEMGELGTGPEPGPAAVAVGALHVGSGAV
                     GNPHIVLHLGSLDGVDPSVEGPLIEADVPGGVNVHFLAASGPDNIRLLHWERGAGVTE
                     ACGSGASVSAALAHRWGLVGERVTVEMPGGTAVVDVGPTLALTGEAVHIADIEVANAW
                     L"
     gene            4560..>5930
                     /gene="hflX"
                     /locus_tag="PKY13_11855"
     CDS             4560..>5930
                     /gene="hflX"
                     /locus_tag="PKY13_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT80678.1"
                     /translation="MPGSDHDSFEFEPDFSDDELLATIFGDAANDPDGPDAGHDDPDA
                     PESHRGAFGDFGATSSAFIDRSFREKIVLVGVSFPPFDDDLVEEHLDELARLIDTAGA
                     DEVGRVVQRRQSPDAATFIGSGKAQEIKEMAIAFDCDTVVFDDELTPAQQFNLEKILG
                     RTAIDRTAVILDIFAQNASSLEGKAQVELAQLKYRLPRLRGKGRSLSQQGGGIGTRGP
                     GETQLEVDRRRLVRRIHKLEEQLRGITHVRDTQRKARRRSLLRNVVIVGYTNAGKSTL
                     LNRLTDSGVLVEDRLFATLDPTTRRLQLPGGESVLMTDTVGFVRKLPHQLVEAFKSTL
                     EVVNDADLLVHVVDGSAVDPQAEIEAVRDVLREIDADGLPELLVFNKCDASEESARLA
                     ERFEGAVAISAVTGEGVEALLATIGDRLRALVAPVDLEIPYDRGDLIAAVHRSGEVLS
                     ETPGEHS"
BASE COUNT          891 a         2103 c         2015 g          921 t
ORIGIN      
        1 cgcatcgagt ccatcaagga tggggtcgag gtcgtgggca accgcacccg ggtcaaggtc
       61 gtcaagaaca aggtggcccc gccgttccgt caggccgagt tcgacatcat gtacgggcag
      121 ggcatcagcc gtgagggttc gctgctcgac atcggtgtcg agatgggctt cgtgaagaag
      181 tccggtgctt ggtacaccta cgaaggcgag cagttgggcc agggccgcga gaacgcgaag
      241 tccttcctca aggacaaccc cgagatcatg gtcgaggtgt ccgagaaggt ccgggccaac
      301 atgggcatcg gtgatctccc cgagggcgat gccgacgacc agccgatcga cctgtcgttg
      361 aacgacgacg tcccgatcac tctcgacgac tgacggctga cgactgacgg ctgagccgtc
      421 gacgtttcgt tcctcgcgcc gacgcttctc ccgacgtgaa acccccgtgg tggaggtttc
      481 acgtcgggag aatgcccggc atcaagatcg tggggatccc gccactggtt ggcgggtgac
      541 ctcacgcgac gcgaagcccg tcgctcgctt tcctcggcag cgcgacgggc ttcgtgctgt
      601 ccgtccggag gtcgctggac ccggcggcaa cttccgacgt cgaacccgcg ccggacgggt
      661 tccacgccgg gaagacggat ggggaccggc cgtcgcgtac gctgtcgggg tgtccaagcg
      721 gtacctgatc cgcacccacg ggtgtcagat gaacgagcac gactccgagc gaatcgcggg
      781 gttgttcgaa gcggacggca tggtgccagc cgacggcatc gacgatgcgg acgtggtggt
      841 gctcaacacc tgctgcatcc gtgagaacgc tgacaacaag ctgtacggca ccctcgggca
      901 cctcaaatct gtcaaggccg cccggcccga gttggagatt gtggtcggcg gctgcctcgc
      961 ccagaaggac cgcgacctca tccaggagaa ggccggccac gtcgacgtcg tcttcgggac
     1021 gcacaacgtg caccgcgccg tcgacctgct cgaacagtct cggcgagagg ggccggtcac
     1081 cgagatcctc gaggcagccg tcctcgacga ccgcgacctg ttcccgagcg cgctcccggc
     1141 gcgccgcgag gtcggctacc tcgcgtgggt gacgatccag atcgggtgcg acaactcgtg
     1201 tgcgttctgc atcgtgccgt cggtacgcgg cgacgagatc agcaagccgt tcggttcgat
     1261 cgtcgacgag gtccggcaac ttgcggccga cggcgtgacc gaggtgaccc tcctcggcca
     1321 gaacgtgaac tcctacggcc gcgacctcac cctcaagctc cgtaacgagc ccggcgagct
     1381 cgaccggtcg gccgtcgccg gcgtcacctg ggacgccgat gggcgggccc gttcgctctt
     1441 cgcggacctg ctcgccgagg tcggagcggt cgagggcatc cgccgggttc ggtacacgag
     1501 cccgcacccg aaggacttcc gcgacgacac gatcgccgcc atggccgaca ctgcggccgt
     1561 gtgcgaacac ctccacctgc cgctgcagtc gggcagcgac acgatcctgg cggcgatgca
     1621 ccggggttac accgccgagc gctatctcca gaaggtcgct gccgctcgtg ccggcatcgc
     1681 cgacctcgcc gtgacgaccg acatcatcat cgggttcccc ggcgagaccg agtccgactt
     1741 cgaggcgacc ctcgcggtcg cggccgaagt cggctacgac tcggcgtaca cgttcgtgta
     1801 ctcgccccgt cccggcaccg aggccgccga gctgaccgat cgcttcgtcg agcggtccgt
     1861 gaccgtggag cggatgaacc ggctccgcga cgtcgtcgag cggtcgtcgg tgcagcgcaa
     1921 cgctgcacgc gtcggtcgcg tcgaagaggt catcgtcgaa ggtccgtcga agaagaaccc
     1981 cggcgtgctg acggggcgaa cccgccagaa caagctgtgc catttcgctg cgccgcagcc
     2041 gatccgggcc ggcagctacg cgctggtcga ggtgaccggt tccgcgctca gccacctgac
     2101 cggtgagttc cgcgaagtca cccacgtcgc acagcaccgc acgcgcatcc ccgtcgccgc
     2161 gatctgaccg ccgtgggagt cttcgattcg ccgcgcgacg ggacgccggc cgactcgccg
     2221 acggcccgtc tccgtcccga tgacctggtc gtcgcgctcg cccgcttcct ccaccgtgtc
     2281 cacacgtcgg cgccggtcga cggggccgag gccctgtcgg cgacgccctc ggactacctg
     2341 gcgctcgccg ccgagcgggt cgaatccggg gccgtgacgt ccgactcgtt cgacgagccg
     2401 taccgaccgc acgctcccga gcggctgctg tcgatcgcaa ccgatctcgc cgacggcctc
     2461 ggggccgtgg cgtcggtccc ggtgcacggg tcgctggcgc tcgccgacct gcggatcgac
     2521 ggtggatcgg tggtgggttg gaccgtcccg gccaccccac gtcacggcga ctcgtacgtc
     2581 gacctctcat tcctcgcccg agacctcgct gcggccatcg ggccggccgc ggtcccggcg
     2641 ctcttcgacg cctacggtgc cgaacggccc gacccggtgc gcatcgagtt ctgggtcacc
     2701 ctccgccaac tgctttcatg actgcctcgg ccacctccgg gtcaggctcg ctccctcgtc
     2761 cctcgggaac tgcttcccct gcggtacctc ggcgtgacgt gactgtctgc cctcgctcga
     2821 tcgtcatcgt cggcccgacg gcatcgggca agtcggcgct ggcactcgag ttggcccggc
     2881 tcctggggga cagcgagatc gtctcggtcg actcgatgca ggtctaccgg cgcatggaca
     2941 tcggcacggg aaaggtcacg accgacgagc gcgccgagac gccgcaccgc cttctcgacc
     3001 tcgtcgaacc gtccaccgag ttctcggtgt catggttcca atccgaggcc cgggccgtgc
     3061 tcgccgatct tgctgagcgt ggtcgccgcg ccatcctggt cggcggcacc gggctctacc
     3121 accgggcggt cgtcgacgac ctggagattc ccggcgagta tccggagctg cgcagcgaac
     3181 tcgagcatga ggctgccgcc ggccccgacg cggtcgcggc actacaccgc cggctcgcag
     3241 tgctggaccc aaccgctgcg agtcgctgtg agccgaccaa cgcccgccgg gtcgttcgcg
     3301 ccctcgaggt caccctgggc tcgggtcgcc cgttctcgtc ctacgggccg ggcctcgagg
     3361 agtacccgcc gtcggaccac ctgatggtgg gactgcaggt cgagcgttcg acgatgctgc
     3421 cgctcatcga gcatcgggtg cacacgatga tggcggccgg gttcgtcgac gaggtgcggg
     3481 cgctggcagc cgaggagccg cccctgtcgc ggaccgctcg gcaggcgctc ggctaccgcg
     3541 agctgctgtc gcatctcgcg ggccgatcga ccgaagtcga ggcggtcgcc gaaacggtcg
     3601 tgcgcacccg tcagttcgca gtgcgccagg accgttggtt ccgccgcgat ccccgaatcg
     3661 agtggctccc gacgacgtgc gacggacccg ccccaactcg ctcgttggcc tcgttcatcg
     3721 ccggtagcgt ggactccaca tgacggcatc cctccgactc acgaaggccc acggcctcgg
     3781 caacgatttc ctggtcgccc tcgagaccgc gaacccggtg ctgaccgccg cgtcggtggc
     3841 agcgggaggt tcgctcgccg ccacagcgct ctgcgaccgc caccggggcg tcggagccga
     3901 cggcctgatc ttcgggctcg ctcccgatgg gtcgggcgcc gatctgcgca tggtcctgtt
     3961 caacgccgac ggctccgagg cggagatctc cggcaacggc atccgctgcc tcggtcaggc
     4021 tgcgctgcgg gccggcggcc gacgcgacgg ccgcatcgtg atcgactcgg gagcgggccg
     4081 ccgcacgctc gacgccacag cgacgacggc gcccgacacc gacctgttgc gcgtcgagat
     4141 gggcgaattg gggacgggcc ccgagccggg tccggccgcc gtcgccgtgg gcgctctgca
     4201 cgtcgggtcc ggtgccgtcg gcaacccgca catcgtgttg cacctcggct ccctcgacgg
     4261 cgtcgacccc tcggtcgagg gcccgctgat cgaggccgac gtgccgggtg gggtcaacgt
     4321 gcatttcctc gcagcgagcg gtcccgacaa cattcggctc ctccactggg agcgcggcgc
     4381 cggggtcacc gaggcgtgcg gatcgggggc gagcgtgtcg gcggccctgg cccaccggtg
     4441 gggtctcgtc ggcgagcgcg tcacggtgga gatgccgggt gggaccgccg tcgtcgacgt
     4501 cggaccgacg ctggcgttga ccggcgaagc cgtgcacatc gccgacatcg aggtggcgaa
     4561 tgcctggctc tgatcacgac tccttcgagt tcgagccgga cttctccgac gacgagctcc
     4621 tcgcgacgat cttcggcgac gccgcgaacg accccgacgg acccgacgcc ggacacgacg
     4681 accccgacgc tccggagtcc caccgcggcg cgttcggcga cttcggcgcg acgtcgagcg
     4741 ccttcatcga ccggtcgttc cgggagaaga tcgtgctcgt cggcgtgtcg ttcccgccgt
     4801 tcgacgacga cctggtcgag gaacacctcg acgagctcgc acgcctgatc gacaccgccg
     4861 gggccgacga ggtgggccgc gtggtgcagc gtcggcagag tcccgacgcc gccacgttca
     4921 tcggctccgg caaggcgcag gagatcaagg agatggcgat cgccttcgac tgcgacaccg
     4981 tggtcttcga cgacgagctg acgccggcac agcagttcaa cctcgagaag atcctcgggc
     5041 gaaccgcgat cgaccgcacc gcggtgatcc tcgacatctt cgcccagaac gcgtcgagcc
     5101 tcgagggcaa ggcgcaggtc gagttggccc agctcaagta ccggcttccc cggttgcggg
     5161 gcaagggccg aagcctgtcc cagcagggcg gtggcatcgg cacccgcggc ccgggcgaga
     5221 cgcagctcga ggtcgaccgt cgtcgactgg tccggcgcat ccacaagctc gaggaacagc
     5281 tgcgcggcat tacccacgtg cgcgacaccc aacgcaaggc ccgtcgccgt tcgctcctcc
     5341 gcaacgtggt gatcgtcggg tacaccaacg cgggcaagtc cacgttgctc aaccgcctca
     5401 ccgactccgg tgtgctcgtc gaggaccggc tcttcgcgac gctcgacccc accacccgac
     5461 gtctgcaact gcccggcggc gaatcggtcc tgatgaccga caccgtcggg ttcgtgcgaa
     5521 agctgcccca tcagctggtc gaggcgttca agtcgacgct cgaggtcgtc aacgacgccg
     5581 acctgctggt ccacgtggtc gacgggtcgg cggtggatcc gcaggccgag atcgaggccg
     5641 tacgagatgt gctgcgggag atcgacgccg acggccttcc ggagctgctc gtgttcaaca
     5701 agtgcgacgc gtcggaggaa tcggcgcgac tcgccgagcg gttcgagggc gccgtcgcga
     5761 tctccgctgt caccggcgaa ggggtcgagg cgttgctggc caccatcggc gaccgcctgc
     5821 gggccctggt cgcgcccgtc gacctcgaga ttccctacga ccggggcgat ctgattgccg
     5881 cggtgcaccg gtccggtgag gtgctgtcgg agacgccggg tgagcattcg
//
LOCUS       DAOSOA010000202         5922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13589_length_5922_cov_5.333049, whole genome shotgun sequence.
ACCESSION   DAOSOA010000202 DAOSOA010000000
VERSION     DAOSOA010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13589_length_5922_cov_5.333049"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKY13_11860"
     CDS             complement(<1..185)
                     /locus_tag="PKY13_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80679.1"
                     /translation="MTDLLAYVDESARPGRYLMCSMLVDPARAGTLRRHVRRLLLPGP
                     SRLHFSKEGTRHRRQIAS"
     gene            complement(187..1326)
                     /locus_tag="PKY13_11865"
     CDS             complement(187..1326)
                     /locus_tag="PKY13_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT80680.1"
                     /translation="MRGHVHKRGSRWAYVVDVGRDPATGKRKQRTKGGFATKAAAEEA
                     LGDFMSELRAGTAVAPTSVTLEGYLTEWLQTVKPQLRETTWASYVVAVGRITWGLGSR
                     KLQALTAIEVERFYAKLVNEGGPAGRPLAPKTVRNTHIVLHRALADAERLRLVMRSVA
                     HLARPPAVPHVERATWTADELRTFLQHVDGDRLFIAYVLLATTGMRRGEVFGLRWRDV
                     DLVARRASVTQTITTVNDKVIIGPTKTNRSRRTISLDPTTIEALEAHRVAQEAERAIA
                     GDAWDPSFDLVICDGTGRPEHPDRFSRQFQRYVGETKLPALRGPHNLRHTWATLALNA
                     GVHPKVVSDRLGHATIAVTIDTYSHVAPSLDAAAADTVAANIFGT"
     gene            complement(1428..1512)
                     /locus_tag="PKY13_11870"
     tRNA            complement(1428..1512)
                     /locus_tag="PKY13_11870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1477..1479),aa:Leu,seq:taa)
     gene            1570..1947
                     /locus_tag="PKY13_11875"
     CDS             1570..1947
                     /locus_tag="PKY13_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT80681.1"
                     /translation="MEHDSIEQRLADERNVWLATSRDGHPPHLVPIWFVWLDERFWVC
                     TSQSVKSRNIADHAEVTLALEDGNAPVVVEGTATLRERPYPEPVCAAFGGKYQWDINR
                     PDEDGDYDVLIEIDPTRWVFGSP"
     gene            complement(1965..2465)
                     /gene="tpx"
                     /locus_tag="PKY13_11880"
     CDS             complement(1965..2465)
                     /gene="tpx"
                     /locus_tag="PKY13_11880"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOT80682.1"
                     /translation="MASITLGGNPIETSGDLPATGTVAPEFHLAGADLSTISSSDVAG
                     QRVVLNIFPSVDTAVCAMSVRKFNEAAGNLDNTTVLCVSADLPFAAARFCGAEGLDQV
                     KTASTFRSPAFGEAYGVTILDGAFAGLLARSVVVLDTDGKVLHSELVPEIAEEPNYDA
                     AIAALG"
     gene            complement(2504..3652)
                     /locus_tag="PKY13_11885"
     CDS             complement(2504..3652)
                     /locus_tag="PKY13_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HOT80683.1"
                     /translation="MTDAPEVQKLTTSSRDRNETRASLEQWLIERLGPDADARITEFV
                     VPEGNGMSSETILFDADWTEDGERSNHRLVMRLAPAADSFPVFPKYDLTQQFETIRLI
                     AEHTDIPVPEVLWDEPGLETLGAPFFVMRRVDGIVPRDVMPYTFGDNWVYDASPEQLW
                     TLTHSTAEVLAKLREIPDPTTTFALLEQPGHESESPLRRHVNDSRAWLDWSVGDEPSP
                     LLDRCFQWLEDHWPEVEPPATVSWGDARIGNIMYDDFRPVAVLDWEMAALGPAEIDLA
                     WFIFLHRFFDDLAVLFGMEGMPNFVDRDEVIAAYTAISGYTPTDMDWYLMYAATRHGI
                     VFTRTSRRAAHFGDAPMPDNPDDAIMHAPTLEAMMAGTYWSDLAARQK"
     gene            complement(3720..4646)
                     /locus_tag="PKY13_11890"
     CDS             complement(3720..4646)
                     /locus_tag="PKY13_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOT80684.1"
                     /translation="MRYLVTHSTTYRSPEPVTIGYHQAHLSPRDTPGQRVVSHQLDIS
                     PTPADRADHVDLYGNRFTYFCVSEPHRVFDVTATSEIEILKRRPLANPVSGWERAVDH
                     ALADPLVCDFVTPSPLIAANAAISSYAAPSFPAGRPLLECVNDLNHRIYTDFNYRPGW
                     TTVSTPLAEVIESRRGVCQDFAHLAIACVRSMGLAGRYVSGYIENLPPEGQPKMVGAD
                     ASHAWASVRDAEGMWIDLDPTNDLVEPVSHITVAWGRDYSDVVPLKGVVTSTGGRTVM
                     SVAVEVRRRTPADERKPRAGAHDEVVAPTSAA"
     gene            complement(4643..>5922)
                     /locus_tag="PKY13_11895"
     CDS             complement(4643..>5922)
                     /locus_tag="PKY13_11895"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOT80685.1"
                     /translation="AFMVRDHDGYRCMEGGLTLTHDRPESITFNNHLISKDTWVTSPT
                     HVPSHDPVVRPSAGLAPIDLRSSITSRAAESMFWIGRNLERSHAAIRLVRAVSLMNET
                     RYDLKDDSDWTWTWTVDRLVNAVVGVPWPPPEDADSAPEPTTAELLTGALVDRSRVRS
                     LSTSLYFLMQNAASVRELFSADSWRLLSQISGHHAQLGADDPEAAAERAYATVTPLLA
                     LSGVISDSMVRDPGWRFLDIGRRIDAASLVATTLGAALVDDPPSHLASPFLDAILNTW
                     DGLIAYRRRYRSDVDPALLIDLLVTDQSNPRSVRSQLSALIVDLEELPGAATRRAEIV
                     DTVRMLAALVDATTPEALAAVDDHGRRPALALATDSIGATLGAVATEIDLTYFVQLPP
                     TSLFSELIVDSGDLDPDEFGALGIVADSEAGAS"
BASE COUNT          900 a         1902 c         2163 g          957 t
ORIGIN      
        1 gacgcaatct ggcggcggtg tcgggtgccc tccttgctga agtgcagacg cgacggaccg
       61 gggaggagca gccgcctgac gtgccgacga agtgtccccg cccgtgcggg gtcgaccagc
      121 atggaacaca tcaggtaccg cccggggcgc gcgctctcgt ccacgtaggc gagcaggtct
      181 gtcacgtcac gtcccgaaga tgtttgccgc gacggtgtcg gcggcggctg cgtcgaggct
      241 gggagctacg tggctgtagg tgtcgatggt gacagcgatg gtcgcgtggc ccaaccgatc
      301 ggacacgacc ttcggatgca ctcccgcatt gagcgcgagc gtcgcccaag tgtggcgcag
      361 attgtgcggc ccccgcagcg ccggcagctt ggtctcgccg acgtaccgct ggaactgacg
      421 cgagaagcgg tcgggatgct cgggtcggcc ggtcccgtcg cagatcacca gatcgaagga
      481 cggatcccac gcgtcacccg cgatcgctcg ctccgcctcc tgcgcgactc gatgcgcttc
      541 gagcgcctcg atggtcgtcg gatcgagcga gatcgtgcgc cggctccggt tggtcttcgt
      601 cggaccgatg atcaccttgt cgttcacggt cgtgatcgtc tgcgtcaccg aggcacgtcg
      661 agcgacgagg tcgacatctc gccatcgcaa cccgaacact tcgccgcgac gcatcccggt
      721 ggtggcgagc aacacgtacg cgatgaacag acgatcaccg tcaacgtgtt ggaggaacgt
      781 ccgcaactcg tcggcggtcc acgtcgctcg ctcgacgtgg ggcacggccg gcgggcgggc
      841 gaggtgcgcc acgctgcgca tcacgaggcg cagtcgctcg gcgtcggcga gcgcccggtg
      901 caacacgatg tgggtgttgc ggaccgtctt cggggcgagg ggtcggccgg ccggtcctcc
      961 ttcgttgacg agcttggcgt agaagcgctc gacctcgatc gccgtgagcg cttggagctt
     1021 gcgggacccg aggccccacg tgatccgacc cactgcgacg acatagctcg cccaggtggt
     1081 ctcacgcagt tggggcttca ccgtctggag ccactcggtc aggtacccct cgagcgtcac
     1141 cgaggttggc gcgacggccg tgcctgcccg cagctccgac atgaagtccc cgagcgcctc
     1201 ctcggccgcc gccttcgtgg cgaacccgcc cttggtgcgc tgcttacgct tgccggtcgc
     1261 agggtcgcgc ccgacgtcga cgacgtacgc ccaccgactc cctctcttgt gaacgtgccc
     1321 gcgcatcggg agagggtacc gcggctccgg ttagcaatgc gttagcaatt cgaggtttgg
     1381 actgggcgac accagacgga tgatcgttca gaaccctgtg tttgcggtgc ccggggtggg
     1441 tttcgaaccc acacgtcccc ggagggaccg aggggtttaa gccctctgcg tctgccattc
     1501 cgccacccgg gcgggcgcga tcaacggtag accgtaggtg ctggtccccg atcgtgggag
     1561 gaacgaacga tggagcacga ctcgatcgag caacgcctgg ccgacgaacg caacgtgtgg
     1621 ctggccacca gtcgcgacgg ccacccgccc catctcgttc cgatctggtt cgtgtggctc
     1681 gacgagcggt tctgggtctg cacgtcgcag tccgtgaagt cgcgcaacat cgccgaccat
     1741 gcggaggtga cgctcgcgct cgaggacggc aacgcgcccg tcgtcgtcga gggcacggcg
     1801 acgttgcgcg agcggccgta tcccgaaccg gtctgcgccg cgttcggcgg gaagtaccag
     1861 tgggacatca accgacccga tgaggacggc gactacgacg tcctcatcga gatcgatcca
     1921 acacgatggg tgttcggctc gccctgaccg aggtggcgcg gtcgtcagcc gagcgctgcg
     1981 atcgctgcgt cgtagttggg ctcctcggcg atctcgggca cgagttcgct gtggagcacc
     2041 ttgccgtcgg tgtcgagcac gacgacagac cgggcgagga gcccggcaaa cgccccgtcg
     2101 aggatggtca cgccgtaggc ctcaccgaag gcgggggaac ggaacgtcga cgccgtcttg
     2161 acctggtcga ggccctcggc accgcagaac cgcgccgccg cgaacggcag gtcggccgac
     2221 acgcacagca ccgtggtgtt gtcgaggttg ccggcggcct cgttgaactt ccgcacgctc
     2281 atcgcgcaca ccgcagtgtc gacgctcggg aagatgttga gcacgacccg ctggccggcg
     2341 acgtcgctcg acgagatggt ggagaggtcg gcgccggcga ggtggaactc gggagcgacg
     2401 gtgccggtgg cgggcaggtc gcccgaggtc tcgatcgggt tgccgccgag ggtgatgctg
     2461 gccatgggga actcctggtg tggtggtggg ctgcgcccgg accctacttc tggcgtgcag
     2521 cgagatccga ccagtaggtg ccggccatca tcgcctcgag ggtgggggcg tgcatgatgg
     2581 cgtcatcggg gttgtcgggc atcggcgcat cgccgaagtg ggcggcgcgc cggctggtgc
     2641 gggtgaacac gatgccgtgc ctcgtggccg cgtacatgag gtaccagtcc atgtcggtcg
     2701 gcgtgtaccc cgagatggcg gtgtacgcgg cgatgacttc gtcgcggtcg acgaagttgg
     2761 gcatgccctc catgccgaac agcacggcga ggtcgtcgaa gaagcggtgg aggaagatga
     2821 accaggccag gtcgatctcg gcggggccga gcgccgccat ctcccagtcg agcaccgcga
     2881 ccggtcggaa gtcgtcgtac atgatgttgc cgatgcgggc gtcgccccag ctcacggtgg
     2941 cgggcggctc cacctcgggc cagtggtcct cgagccactg gaagcagcgg tcgagcagcg
     3001 gtgacggctc gtcgccgacc gaccagtcga gccaggcccg gctgtcgttg acgtgacgtc
     3061 gcaacggcga ctccgactcg tgtccgggct gttcgagcag ggcgaacgtg gtcgtcgggt
     3121 cgggaatctc ccggagcttc gccagcacct cggcggtgga gtgcgtgagg gtccacagct
     3181 gctccgggct ggcgtcgtac acccagttgt cgccgaacgt gtacggcatg acgtcacgag
     3241 ggacgatgcc gtcgacccgt cgcatcacga agaacggtgc gccgagcgtc tcgaggcccg
     3301 gctcgtccca cagcacctcg ggcaccggga tgtcggtgtg ctcggcgatg agccggatcg
     3361 tctcgaactg ctgggtgagg tcgtacttgg ggaagacggg gaacgagtcg gcggcgggcg
     3421 cgaggcgcat cacgaggcgg tggttcgacc gctcgccgtc ctcggtccag tcggcatcga
     3481 acaggatcgt ctcactcgac atcccgttgc cctcgggcac gacgaactcg gtgatgcggg
     3541 cgtcggcatc ggggccgagc cgctcgatga gccactgctc gaggctggcg cgcgtctcgt
     3601 tgcgatcgcg cgacgacgtc gtcagcttct gcacctccgg tgcatcggtc atgttctccc
     3661 cctggtcatt cccaacgtga acgtgagact aacggcgatc gacctcggtc ggggccgggt
     3721 caggcggcgc tcgtcggagc gacgacctcg tcgtgagccc ctgcccgtgg cttccgctcg
     3781 tctgcgggcg tgcggcgccg aacctcgacc gcgacgctca tcaccgttcg cccaccggtc
     3841 gacgtgacga cccccttgag gggcacgacg tcggagtagt cgcggcccca cgccacggtg
     3901 atgtgcgaca cgggttcgac gagatcgttg gtcgggtcga ggtcgatcca catcccctcg
     3961 gcgtcgcgca ccgacgccca ggcgtgcgat gcgtcggcgc cgaccatctt cggttgcccc
     4021 tccggcggga ggttctcgat gtagccgctc acgtaccggc cggcgaggcc catggagcgg
     4081 acgcaggcga tggcgaggtg agcgaagtcc tggcacacac cgcgccgtga ctcgatcacc
     4141 tcggcgagcg gtgtcgacac ggtcgtccac cccggccggt agttgaagtc ggtgtagatg
     4201 cggtggttca ggtcgttgac gcactcgagc agcggccgac cggccgggaa cgacggcgcc
     4261 gcgtacgagg agatcgcagc gttggcggcg atgagcgggc tcggggtcac gaagtcgcac
     4321 acgagcgggt cggccagggc gtggtcgacc gcccgctccc accccgacac cgggttggcg
     4381 agcgggcgac gcttgaggat ctcgatctcg ctcgtcgcgg tcacgtcgaa cacccggtgg
     4441 ggttcggaca cgcagaagta ggtgaaccgg ttgccgtaca ggtcgacgtg atcggcacgg
     4501 tccgcgggtg tgggcgagat gtcgagctga tgggacacca cccgttgacc cggcgtgtcg
     4561 cggggcgaca ggtgggcctg gtgatatccg atcgtgaccg gctcgggcga gcggtaggtc
     4621 gtggagtgcg tgacgaggta cctcacgatg cgcccgcttc gctgtcggcg acgatgccga
     4681 gggccccgaa ttcgtcgggg tcgagatcgc ccgagtcgac gatcagttcg ctgaacaggc
     4741 tcgtcggcgg cagctgcacg aagtacgtga ggtcgatctc ggtggcgacc gccccgagcg
     4801 tggccccgat gctgtcggtg gcgagggcga gcgccggacg tcggccgtgg tcgtcgacgg
     4861 ctgcgagggc ctcgggcgtt gtggcgtcga ccaacgcggc gagcatccgc acggtgtcga
     4921 cgatctcggc ccgccgggtg gcggcgccgg gcagctcctc caagtcgacg atcagcgccg
     4981 acagttgtga tcggaccgat cgcgggttgc tctggtcggt gacgaggaga tcgatcagca
     5041 gcgccggatc gacgtcgctg cggtagcggc ggcggtacgc gatgaggccg tcccaggtgt
     5101 tgaggatggc gtcgaggaac ggcgacgcga ggtgcgacgg tgggtcgtcg acgagcgccg
     5161 ctccgagcgt ggtggcaacg agcgaggcgg catcgatgcg gcggccgatg tcgaggaaac
     5221 gccaccccgg atcgcgcacc atcgagtcgc tgatcacgcc cgacagggcg agcagcggcg
     5281 tcaccgtggc gtaggcgcgc tctgcggccg cctcgggatc gtccgcgccg agctgggcat
     5341 ggtgaccgct gatctggctg agcagccgcc acgagtccgc cgagaagagc tcccggaccg
     5401 acgccgcgtt ctgcatcagg aagtagagcg acgtcgacag gctgcggacc cggctgcggt
     5461 ccacgagcgc gccggtcaac aactcggcgg tcgtcggctc gggcgccgag tcggcgtcct
     5521 cgggcggcgg ccacggcaca cccaccacgg cgttgaccag gcggtcgacc gtccaggtcc
     5581 acgtccagtc gctgtcgtcc ttcaggtcgt agcgggtctc gttcatgagg ctcacggccc
     5641 ggacgaggcg gatcgcagcg tgggagcgtt cgaggttgcg gccgatccag aacatcgact
     5701 cggcggcccg actggtgatc gaactgcgga gatcgatggg ggccagtccc gcgctcggcc
     5761 ggacgaccgg atcgtggctc gggacgtggg tcggcgaggt gacccaggtg tccttgctga
     5821 tcaggtggtt gttgaacgtg atcgattcgg gccggtcgtg ggtcagcgtc aggccgccct
     5881 ccatgcaccg gtacccgtcg tggtcgcgga ccatgaatgc cc
//
LOCUS       DAOSOA010000203         5895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13675_length_5895_cov_5.694692, whole genome shotgun sequence.
ACCESSION   DAOSOA010000203 DAOSOA010000000
VERSION     DAOSOA010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13675_length_5895_cov_5.694692"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKY13_11900"
     CDS             complement(<1..684)
                     /locus_tag="PKY13_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT80686.1"
                     /translation="MAVFIVRRLFSLAVTLIGGFTLLFLLFFTLPSDPARVISGGNGR
                     NPSPAVIANTERRLGLDKPIYEQYWNRLTDTVTLSGISFQTREPVRDMVAERLPASLR
                     LAVWAMLIEASVGVGSGVLSARRRNSIADTVTTTAAVVAGAIPVFVLGYLLTLVTGVY
                     AFKHNWPEWAHFPTLSTNYDADEWYLFFIPNLKQLELLVQPAIILASVSTAIVARLTR
                     TTMLEAQTAD"
     gene            complement(748..2511)
                     /locus_tag="PKY13_11905"
     CDS             complement(748..2511)
                     /locus_tag="PKY13_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT80687.1"
                     /translation="MRKRLGSSNAGRGVAALALAFTLVAAGCGDSKDSSDGESSGGDT
                     AASTTLPPRSDSCRTLKYEDAPEGGEFVDYAQLASAGDNTSFDPGAVQTLDESQITGA
                     LFDGLTDFDFTDTCNPELKPLVAESFEANDDATVFTFKIKDDQKFSNGEPVLPHNFKQ
                     GWERAGSQELASSYGYLMAYVKGGADLLDGKVDTLDSIVADDDAMTLEVTLESPLADF
                     PAIVSFSSFSPIADEDIARVGNTTGWGDKGAVIGNGPFKFESAGSPDSGEVVLVRNDD
                     WAGNVLGDTKAKLDKITFKLTTDVESAYQAFSAGEGDNATIPSGKYKEATATYPNDTK
                     SPQLGTYFFDIGHDDPVLGGEKNTKLRQAISMAIDRDEINTKAYEGARIVSTGITPPG
                     IPGFKADIGKYAKTDAAEAKKLYDEWVADGGTLDGPIKISFNTGGSHQTVVEIMQANL
                     KDVLGIDVELNAIDEDYFKVIAQPGACQVCRSGWYADYPTYGNFMVDLFGKVSIGGNN
                     LGGYDDEKFEENLSNALKETDPTKRGEFYQAAEERLLNETTNAIPLNWYTGDQVYRDT
                     VINYDQQPLGNILWEKIGKKG"
     gene            complement(2719..3831)
                     /locus_tag="PKY13_11910"
     CDS             complement(2719..3831)
                     /locus_tag="PKY13_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HOT80688.1"
                     /translation="MSTKMRAALLDEVGAPMIVVDDVELDGPGLGEVRVEVAWCGVCH
                     SDVSQVDGVHPALTPSVLGHEAAGVVVEVGSGVTQLAVGDHVVLSPSAACGHCYWCVR
                     NEHSSCVNSAAIAMSMLPDGSTRLSRQGSVVYRGLGLAAMAEYVVVAESAAIKIDADL
                     PLDLACIIGCAVQTGVGAALNTAKVEAGATVLVMGAGGIGSSIVQGARIAGATRIIVS
                     DPVEARRESALQVGATDVVDPTKDDVISVAQALTGVGVDYAFDAVGHAALVETGVTAT
                     RNGGTTVIVGAAPVDHEAKLNVVLTMFTEKRVVGTLLGGCHAPRDFPRLVALWQAGRL
                     DLDSMVTNRQPLAEVNEAMADMRAGLGVRTVLDVRS"
     gene            complement(3842..4741)
                     /locus_tag="PKY13_11915"
     CDS             complement(3842..4741)
                     /locus_tag="PKY13_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80689.1"
                     /translation="MSHRIAIVTSGPGRPFDDDLPPLVAALEQVGAAVEVLDWDDDGA
                     DWASVDLAVIRSTWDYTQRHDEFLDWTRRCASATTLINAPAVVAWNSDKRYLRDLVDA
                     GVNVVDTSFIEPGDAIELPVGVEFVVKPTVSAGSRDTVRYHADDHTPATAQIEALLAD
                     GRTVMVQPYQAAVDEQGETALLFFGGEFSHAIRKGPLLVAGEEATRALFAQEVITATD
                     AHPDQIALATQCLAALGGVDALAGVSQPLPYARVDVLVDNDGRFSILELELTEPSLFF
                     DWAPGAPDRYARVLVELAEAARS"
     gene            4836..>5895
                     /locus_tag="PKY13_11920"
     CDS             4836..>5895
                     /locus_tag="PKY13_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT80690.1"
                     /translation="MLKIHWFLPTGGDSRDVSPSDDGSPVRPPTIDYLSQIARACDQL
                     GFDAVLTPCGTGCEDSWITTAMLVPQTRRLRFLVAFRPGLLSPTLAAQMASSYQRLSG
                     GRVIVNLVTGAEPRELARFGDYADKDARYARTGEFIEVMRGAWGAEPFDHEGPNYRVE
                     AATTREQPTPQPEIYFGGASEAAEVVAAAGADVYLAWGEPPEMVAERVDRMRKLAADN
                     GRTVRLGIRFHTIARPTSEEAWAEAGRLQAGMSPEAIAAARADFETTQSEGQRRMAEL
                     NGARSNVTAGDLSDPRSLEIYPNVWSGIGLVRGGAGTALVGSFTEVADRIAEYAALGF
                     EEFILSGYPHLEEAWWVGE"
BASE COUNT          913 a         1945 c         2071 g          966 t
ORIGIN      
        1 gtcggcggtc tgcgcctcga gcatcgtggt gcgggtgagc cgggccacga tggccgtcga
       61 caccgacgcc aagatgatgg cgggttgtac caagagctcg agctgcttga ggttcgggat
      121 gaagaagagg taccactcgt ccgcgtcgta gttggtactc aacgtcggga agtgagccca
      181 ttcgggccag ttgtgcttga acgcgtagac ccccgtcacg agggtcaaca ggtacccgag
      241 cacgaagacg gggatggcac cggcgaccac cgcagcggtg gtggtgacgg tgtcggcgat
      301 cgaatttcgt cttcgggcgg agagcactcc cgacccgacg ccgacggagg cctcgatcag
      361 catcgcccag acggcgaggc ggaggctggc ggggagacgt tccgcgacca tgtcgcggac
      421 gggctcgcgg gtctggaagc tgatccccga gagggtgacc gtgtcggtga gccggttcca
      481 gtactgctcg tagatcggct tgtcgagccc gagacggcgc tcggtgttgg cgatgaccgc
      541 cggtgagggg ttgcgaccgt tgccgccgga gatgacccgg gccgggtcgg agggcagcgt
      601 gaagaagagc aggaagagca gggtgaagcc gccgatgagg gtcacggcga gcgagaagag
      661 acgacggacg atgaagacag ccatggcgaa gtcgaaccga gagcggggtg ggcaccggca
      721 ggtgcccacc ccgttcgagg tcgggtgtta gcccttcttg ccgattttct cccagaggat
      781 gttgccgagc ggctgctggt cgtagttgat caccgtgtcg cggtacacct ggtcgccggt
      841 gtaccagttg agcgggatcg cgttggtcgt ctcgttgagg agacgctcct cagcggcctg
      901 gtagaactcg ccgcgcttgg tcgggtcggt ctccttgagg gcgttcgaca ggttctcctc
      961 gaacttctcg tcgtcgtagc cgccgaggtt gttgccaccg atcgacacct tgccgaacag
     1021 gtcgaccatg aagttgccgt aggtcgggta gtcggcgtac cagccggagc ggcagacctg
     1081 gcaggcgccg ggctgggcga tgaccttgaa gtagtcctcg tcgatggcgt tgagctcgac
     1141 gtcgatgccg aggacgtcct tgaggttggc ctgcatgatc tcgaccacgg tctggtggct
     1201 accaccggtg ttgaacgaga tcttgatcgg accgtcgagg gtgccaccgt cggcgaccca
     1261 ctcgtcgtac agcttcttgg cctcggccgc gtcggtcttg gcgtacttgc cgatgtcagc
     1321 cttgaacccg gggatgccgg ggggcgtgat gccggtggag acgatgcgag cgccctcgta
     1381 ggccttcgtg ttgatctcgt cccggtcgat cgccatcgag atggcctggc ggagcttcgt
     1441 gttcttctcg ccgccgagga ccggatcgtc gtggccgatg tcgaagaagt acgtgccgag
     1501 ctgcggcgat ttggtgtcgt tcgggtaggt ggccgtggcc tccttgtact tgcccgaagg
     1561 aatcgtggcg ttgtcgccct cgccggccga gaaggcctgg taggccgact cgacgtcggt
     1621 ggtcagcttg aacgtgatct tgtcgagctt ggccttcgtg tcgccgagca cgttgccggc
     1681 ccagtcgtcg ttgcgcacga gcacgacctc acccgagtcg ggcgaaccgg cggactcgaa
     1741 cttgaacggg ccgttgccga tgacggcacc cttgtcgccc cacccggtcg tgttgccgac
     1801 ccgagcgatg tcctcatcgg cgatcggcga gaacgacgag aaggagacga tcgccgggaa
     1861 gtcggcgagc gggctctcga gggtgacctc gagggtcatc gcatcgtcgt cggcgacgat
     1921 cgagtcgagg gtgtcgacct tgccgtcgag gaggtcggcg ccacccttga cgtaggccat
     1981 caggtagccg taggaggagg cgagttcctg gctgccggcg cgctcccagc cctgcttgaa
     2041 gttgtggggc aggacgggct cgccgttgct gaacttctgg tcgtccttga tcttgaaggt
     2101 gaagaccgtg gcgtcgtcgt tggcctcgaa gctctcggcg acgagcggct tgagctcagg
     2161 gttgcaggtg tcggtgaagt cgaagtcggt gagaccgtcg aagagcgcac cggtgatctg
     2221 ggactcgtcg agtgtctgca cggcgccggg atcgaagctg gtgttgtcac cggcgctggc
     2281 gagctgcgcg tagtcgacga actcgccgcc ctcgggcgcg tcctcgtact tcagcgtgcg
     2341 gcagctgtcc gaacgcggcg gaagggtcgt gctggcggcg gtgtcgccgc ccgacgactc
     2401 accgtcgctc gaatccttcg aatcgccgca gcctgcggcg acgagggtga acgcgagagc
     2461 gagtgcggcg actcccctcc cggcgttcga cgatcccaag cgttttctca tgttgtggtc
     2521 ctccggatgg atgaataccg actagtcgtg acgcggaacc tacaccccgc cccgtgacgt
     2581 tcgggtgaca gtcaccgttt cgtaacagag cgttctctga aacatctgtt ccagatctgg
     2641 acgagttggg gacgcggccc gcgcaatcgg ttgaggcgat cgactcaatc tatcgagctg
     2701 atccgagccg gccgcgcctc agctgcgaac gtcgaggacg gttcgcaccc cgaggccggc
     2761 gcgcatgtcg gccatcgcct cgttcacctc ggccagcggc tgacggttcg tgaccatcga
     2821 atcgaggtcg aggcgccccg cctgccagag cgcgaccagg cgggggaaat cgcggggagc
     2881 gtggcaccca cccagcagcg ttccgaccac gcgcttctcg gtgaacatcg tcagcaccac
     2941 gttgagcttc gcctcgtggt cgacgggcgc agcgcccacg atcacggtgg tgccgccgtt
     3001 gcgggtcgcg gtcactccgg tctcgacgag cgcagcgtgg ccgacggcgt cgaaggcata
     3061 gtcgacgccg acccccgtga gcgcctgggc caccgagatc acgtcgtcct tcgtcgggtc
     3121 gaccacgtcg gtcgcaccga cctggagcgc tgactcgcga cgggcctcga cggggtcgga
     3181 cacgatgatg cgcgtggcgc cggcgatgcg ggcaccctgc acgatcgacg acccgatgcc
     3241 gcccgcgccc atcacgagca cggtcgcgcc cgcctcgacc ttggcggtgt tgagcgcagc
     3301 acccaccccg gtctgcacgg cgcagccgat gatgcacgcg aggtcgagcg gcaggtcggc
     3361 gtcgatcttg atcgccgccg actcggcgac gacgacgtac tcggccatcg ccgccagccc
     3421 gagcccgcgg tacacgacac tcccctgccg gctcaggcgc gtgctgccgt cgggcagcat
     3481 cgacatcgcg atcgctgccg agttcacaca gctgctgtgc tcgttgcgca cacaccagta
     3541 gcagtggccg caggcggcgc tcgggctcag caccacgtgg tcgccgaccg ccagctgggt
     3601 gacacccgac ccgacctcga cgaccacgcc ggcggcctcg tggcccagca ccgagggcgt
     3661 gagggcaggg tgcaccccgt cgacctgcga cacgtcggag tgacacaccc cgcaccacgc
     3721 cacctcgacg cgcacttcac cgagaccggg gccgtcgagt tcgacgtcgt cgacgacgat
     3781 catcggcgct cccacctcgt ccaggagcgc ggctcgcatc ttcgtcgaca tgtctccccc
     3841 ttcaggagcg ggcggcctcc gccagctcga ccagcaccct tgcatatctg tcgggtgcgc
     3901 cgggagccca gtcgaagaag agcgacggtt cggtcagctc cagctcgagg atcgagaatc
     3961 gaccgtcgtt gtcgacgagc acgtcgaccc gggcgtaggg caggggctgt gacacccctg
     4021 ccagcgcgtc gacacccccg agcgcggcga ggcactgcgt ggcgagggcg atctggtcgg
     4081 ggtgggcgtc ggtggccgtg atgacctcct gggcgaacag ggcgcgggtg gcctcctcgc
     4141 cagcgacgag cagcgggcct ttgcggatcg cgtggctgaa ttcaccaccg aagaacagga
     4201 gcgccgtctc gccctgctcg tcgaccgccg cctggtacgg ctgcaccatg acggtccgac
     4261 cgtcggcgag gagcgcctcg atctgcgccg ttgccggcgt gtggtcgtcg gcgtggtagc
     4321 ggaccgtgtc gcgggacccg gccgacacgg tcggcttgac gacgaactcg acgccgacag
     4381 gcaactcgat cgcgtcgccg ggctcgatga aggacgtgtc gaccacgttg acgccggcgt
     4441 ccacgagatc gcggaggtag cgcttgtcgg agttccacgc gacgaccgcg ggggcgttga
     4501 tcaacgtcgt cgccgacgcg cagcgacggg tccagtcgag gaactcgtcg tggcgttggg
     4561 tgtagtccca ggtggagcgg atcacggcga ggtcgaccga cgcccagtcg gctccgtcat
     4621 cgtcccagtc gagcacctcg acggctgctc cgacctgttc gagagctgcg accagcggcg
     4681 ggagatcgtc gtcgaagggg cggccgggtc cactggtcac gatggcgatg cggtgcgaca
     4741 tggcccgaga cggtagcgcc cggcggagac cggctcggct ggattcctga ccttgggggt
     4801 cgggtaacct gagccacgtc ccgaggagcg tgcgaatgtt gaagatccat tggttcctgc
     4861 ccaccggcgg cgactcccgg gacgtttcac cgtccgacga cggctcaccg gtgcgcccac
     4921 ccacgatcga ctacctcagc cagatcgccc gggcgtgcga ccagctcggc ttcgacgccg
     4981 tcctcacccc ctgcggcacc gggtgcgagg actcgtggat caccacggcc atgctcgtgc
     5041 cgcagacccg ccggctgcgc tttctcgtcg ccttccgccc ggggctgctg tcccccacgc
     5101 tcgccgccca gatggcgtcg agctaccagc gcctgtcggg tggccgcgtg atcgtcaacc
     5161 tcgtcacggg cgccgagccc cgcgagctcg cccgcttcgg cgactacgcc gacaaggacg
     5221 cccggtacgc ccgaaccggc gagttcatcg aggtcatgcg gggcgcctgg ggcgccgagc
     5281 cgttcgacca cgagggcccg aactaccggg tcgaggcggc caccacccgc gaacagccca
     5341 ccccccagcc cgagatctac ttcggtggag cgtccgaggc cgccgaggtg gtcgccgccg
     5401 ccggcgccga cgtgtacctg gcgtggggcg aaccgccgga gatggtcgcc gagcgcgtcg
     5461 accgcatgcg caagctcgcc gccgacaacg ggcgcaccgt gcgattgggc atccgcttcc
     5521 acacgatcgc ccgaccgacg tcggaggaag cgtgggccga ggccggccgc ctccaggccg
     5581 gcatgtcccc cgaggccatc gccgcggccc gcgccgactt cgagaccacc cagtcggagg
     5641 gtcagcgccg gatggccgaa ctcaacggcg ctcgttcgaa cgtgaccgcc ggcgacctga
     5701 gcgatccccg atccctcgag atctacccga acgtgtggag cggcatcggc ctcgtgcgcg
     5761 gcggtgccgg cacggcgctc gtcggcagct tcaccgaagt cgccgaccgc atcgccgagt
     5821 acgccgccct cgggttcgag gagttcatcc tctcgggcta cccgcacctc gaggaagcgt
     5881 ggtgggtcgg cgagg
//
LOCUS       DAOSOA010000204         5875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13731_length_5875_cov_4.662371, whole genome shotgun sequence.
ACCESSION   DAOSOA010000204 DAOSOA010000000
VERSION     DAOSOA010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13731_length_5875_cov_4.662371"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PKY13_11925"
     CDS             <1..539
                     /locus_tag="PKY13_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT80691.1"
                     /translation="YRFWFGQWLPDWRPTVDYRQTYSARADLGGGVLLDAIHELDLAL
                     WVAGPDLSVVGATVARLGPLEIDVEDTVRALLTTPDGVPVSIELDYLARQYRRGIEVI
                     GDAATIRYDWATGDLTVSDGDGTTTEHFDTPVADSYVAEMAEFLSTPAAPTNTAAGAL
                     ASLRLAESIRAAALVGAA"
     gene            536..1444
                     /locus_tag="PKY13_11930"
     CDS             536..1444
                     /locus_tag="PKY13_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOT80692.1"
                     /translation="MSVVCVVQARMGSTRLPGKVLMDLGGQPMLAFMLERLASLDGVR
                     LLVATSTGIGDDPIEACARSVGVPVVRGDEADVLGRFRTALDRYPADHVVRLTADCPL
                     IDRNLVEAVVERHLASGADYTSNTLIRTDPDGVDVEAMTARALRIADREATDPTEREH
                     VTPYLYRRPGRFALAAVLGSARLGHLRWTVDTADDLDRVRTMVATGLSSVDRVPLRDG
                     FEPAIPGEVPARFERFDDPADRLVVLRVDGRREGWARLRISDVGVATVESELPADYDT
                     ELERFAAETPQIVTLHRVQMQETVRT"
     gene            1441..2064
                     /locus_tag="PKY13_11935"
     CDS             1441..2064
                     /locus_tag="PKY13_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT80693.1"
                     /translation="MTTNTDPDAERLEQLWQGEFGDRYVERNSVDYPARRGFWERTIA
                     RYSAGTILEVGCAHGDNFRYLDGLVDLGRTCGIDINEHGLLTLRDQSPNVSAVRGAAR
                     HLPVRARSFDTVFTIGLLIHQPDSTLPSVMRELARCSRRWVMFGEYSSVEPTEIEYRG
                     LSGVLFKRDYVSIFEETCPEYRHVDTEELTLEADGFDRVTWGVFERR"
     gene            2064..3791
                     /locus_tag="PKY13_11940"
     CDS             2064..3791
                     /locus_tag="PKY13_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80694.1"
                     /translation="MPARPDLLIAVAPPPTPPDASAVLAVTAASVGTLAGVGVRYAVV
                     DDVIDRADVLGADEEYVRWQLDWFGRLDDTIGATGAAVASAQKLKPVIDSVVIAARLA
                     AAAIDRLGPDGIELVVPSGAAPLDPLHDGHLQFWPRLGDLSLWSSIVPLVAAARGIPL
                     VIRQVDPSTPAAVAAPVFRDRMRASLAARTGLLRHFDIAHWRPRPVAGSTLMLWSGGY
                     GARHLVSVERASGRRVVALERGAPTTRVFEPVPWGRRNLSSPVDVAPLGRGSSTAAAG
                     VRSLLDDIDDRAGVAGVSDLLQSRVEAFAHRIVPVVASAAASLAPDLERLGIDSVVAA
                     NPSSIEEFAALLAAGRLGIDRTLAQHGDHLFSYGGWFVSEFANFDRFLASDATLVEDI
                     PDAARRLGALAPSVRPSEHRGATRPSAVGARRQDGPVCYVPGVLMGDTAVRPSIYFDD
                     SWYHRWHLGLLDWMRSRPDVRFIWKGLPSGDHVDDPIVDAIDAVRNVDYVTQPLPWLL
                     GQVSRVVTDFPSTPAYEALRAGLPTIVLWFPRFAELRPTARVEFGETVRESADEAAAL
                     DHLSKWLVS"
     gene            complement(3784..4833)
                     /gene="pseI"
                     /locus_tag="PKY13_11945"
     CDS             complement(3784..4833)
                     /gene="pseI"
                     /locus_tag="PKY13_11945"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HOT80695.1"
                     /translation="MTTIEIAGRLVGTGQPTFVIAEVSANHGGDLPRVLEMVRVAAAA
                     GADAVKLQTFTADSMTLDVDLPRFVVGPGNPWSGRRLHDLYREAAMPWDWYPEIAAVA
                     ASEGITLFSSPFDPASVDFLVEQGAPALKIASFELTDLPLIAHAASSGLPLVMSTGMA
                     TVEEIDDAVSVARNAGAGGVALLRCNSAYPSPPADMDLRSIVDLANRWEVPVGLSDHT
                     PDDTSAVVAVALGASIVEKHFMLDRGHGGPDSSFSLEPEELTRLVASVRTAEAALGSV
                     RYGPSASDRASLAFRRSLYVVAPLAAGQVVGSTDVRSLRPAGGLEPRHLPDVIGRVAT
                     RDLEVGHPLSWDDVS"
     gene            complement(4830..>5875)
                     /locus_tag="PKY13_11950"
     CDS             complement(4830..>5875)
                     /locus_tag="PKY13_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose
                     hydrolase/GNAT family N-acetyltransferase"
                     /protein_id="HOT80696.1"
                     /translation="HVLVIDDHGSLGAYDADLVLDHNIGADRGDLLGSRYLLLRTEFA
                     DEPPDRPPPGRRVLLAPGGAPSSSTTATFDGIAAGLIAAGAGLVWLRGVDDVVATMAS
                     ADVAVAAAGVTAYELCRVGVPAVLVAVAENQEPVARRLDDAGAAVSADHADSVDRTVE
                     TALGLLDDPDRCATMTRVARRLVDGLGADRVVTAMRSFLLDVRPATPEDLDLTWEWSN
                     DPDVRRQSFESASIPRHRHEEWFARRLADPEGDFLIVESDGVPVGQARFEGTPTTLGY
                     LVGPASRGRGLASPLLVAACRQHFRRHPAGEVIALVKPDNRASVRALDLAGFEPMDPM
                     DGALRFRSRAGTT"
BASE COUNT          861 a         2063 c         2056 g          895 t
ORIGIN      
        1 cgtaccggtt ctggttcggg cagtggctgc ccgactggcg cccgaccgtc gactaccgac
       61 agacctactc ggcgcgcgcc gacctcggcg ggggagtgct cctcgacgcc atccacgaac
      121 tcgacctcgc cctctgggtg gccggccccg acctctccgt cgtcggggcc acggtcgctc
      181 ggctcggccc gctcgagatc gacgtcgagg acacggttcg ggccctcctg accacgcccg
      241 acggcgtgcc cgtgtcgatc gagctcgact atctcgcccg ccagtaccgc cgcggtatcg
      301 aggtgatcgg cgacgctgcg acgatccgat acgactgggc aaccggcgac ctgacggtca
      361 gcgacggtga cggcaccacg accgagcatt tcgacacgcc ggtggccgat tcctacgtcg
      421 ccgagatggc ggagttcctg agcacgccag cggccccgac caacaccgca gccggcgcac
      481 tcgccagcct gcgcctggct gaatcgatcc gcgctgcggc cctcgtgggc gcggcatgag
      541 cgtcgtgtgc gtcgttcagg cccggatggg ctcgacgcgc ctgcccggga aggtgctgat
      601 ggacctcggc gggcagccca tgctcgcgtt catgctggag cggctcgcgt cgctcgacgg
      661 cgtccgcctc ctggtcgcga cgagcacggg cattggcgat gacccgatcg aggcgtgtgc
      721 ccgctcggtc ggcgtcccgg tcgtgcgcgg tgacgaagcc gacgtcctcg gacgattccg
      781 gacggcgttg gatcgctacc cggccgacca cgtggtccgt ttgaccgcag actgcccgct
      841 catcgatcgg aatctcgtgg aggcagtggt cgaacgtcac ctggcgagcg gcgccgacta
      901 cacgtccaac acgctgatcc gaaccgatcc cgacggcgtc gacgtcgagg caatgacggc
      961 tcgcgcgctc cgcatcgccg atcgggaagc caccgatccg accgagcgtg agcacgtcac
     1021 gccctacctg taccgccggc ccggccggtt cgcgctcgcg gccgtcctcg gctcggcacg
     1081 gctcggccat cttcgctgga ccgtcgatac ggccgatgac ctcgaccggg tgcgtacgat
     1141 ggtcgcgacc ggcctgtcga gcgtcgatcg ggtgccgctc cgtgacgggt tcgaaccggc
     1201 gatacccggc gaggtcccgg cccgcttcga gcggttcgac gaccctgccg accgcctcgt
     1261 cgttctccgc gtcgacggcc gacgagaggg ctgggcgcgg ttgcgcatca gcgacgtcgg
     1321 cgttgccacc gtcgagagcg agctgccggc cgactacgac accgaactcg agcgattcgc
     1381 cgcagagacc ccacagatcg tgaccctcca ccgggtccag atgcaggaaa ccgtgcgcac
     1441 atgaccacga acaccgaccc cgacgccgag cgactcgagc agctgtggca gggggagttc
     1501 ggcgatcgct acgtcgagcg aaactccgtc gactacccgg cgcgacgggg attctgggag
     1561 cggacgatcg ctcgctacag cgccgggacc attctcgagg tgggttgtgc ccacggcgac
     1621 aacttccggt atctcgacgg tctcgtcgac ctcggccgaa cctgtggcat cgacatcaac
     1681 gagcacggtc tcctgacgct gcgcgaccag tcgcccaacg tgtcggcggt gcgtggcgca
     1741 gcccggcatc ttccagtccg ggcccgttcc ttcgacacgg tcttcaccat cggtctgctc
     1801 atccaccagc ccgactccac tcttccttcg gtgatgcgcg agctggcccg atgctcgcga
     1861 cggtgggtga tgttcgggga gtactcgtcg gtcgagccca ccgagatcga gtaccggggc
     1921 ctgagtggtg tgttgttcaa gcgggactac gtgagcatct tcgaggagac gtgtcccgag
     1981 taccgtcacg tggacaccga ggaactcacg ctcgaggcgg acggattcga ccgtgtgacc
     2041 tggggcgtgt tcgaacgccg ctgatgcccg cacgtcccga tctcctgatc gcagtcgccc
     2101 cgcctcccac cccacccgac gcttctgcgg tgctggcggt cacggccgct tccgtcggca
     2161 cgctcgccgg cgtcggcgtc cgatacgcgg tcgtcgacga cgtcatcgac cgcgccgacg
     2221 tgctcggggc cgacgaggag tacgtgcgat ggcagctgga ctggttcggc cgcctcgacg
     2281 atacgatcgg tgcgaccggc gccgccgtgg cgagcgccca gaagctcaaa ccggtgatcg
     2341 actccgtcgt catcgccgcc cgcctcgccg cagcggccat cgaccgactc ggaccggacg
     2401 gcatcgagct ggtcgtcccg tcgggggctg caccactcga cccgctccac gatggacatc
     2461 tccagttctg gccgaggctc ggcgacctgt cgttgtggag ctccatcgtc ccgctcgtcg
     2521 ccgcagcgcg agggatcccg ttggtgatcc gccaggtgga cccgtcaacg ccggccgccg
     2581 tcgcggcgcc ggtgttccgc gatcggatga gggcgagttt ggcagcgcgg accgggctgc
     2641 tccggcactt cgacatcgcc cactggcggc cccggcccgt ggccggctcg accctgatgc
     2701 tgtggagcgg cgggtacggc gcccgtcacc tcgtgtcggt ggagcgcgcg tccgggcgcc
     2761 gcgtcgtcgc gctcgagcga ggggccccga cgacccgcgt gttcgagccg gtgccgtggg
     2821 gccgacgaaa tctcagctca cccgtcgatg tcgcgcccct cggtcgagga tcgtccacgg
     2881 cggccgctgg tgtccggtcg ctgctcgacg acatcgacga ccgggcgggc gtcgccgggg
     2941 tgtccgacct gctgcagtcg cgtgtcgagg cgttcgctca ccggatcgtt ccggtcgtgg
     3001 cctcggccgc agcgagcctg gcccccgacc tcgagcgcct cggaatcgac tccgtcgtcg
     3061 ctgcgaaccc gagctcgatc gaggagttcg cggcgctgct tgccgccggg cgcctgggca
     3121 tcgaccggac cctcgcccag cacggcgacc atctcttctc ctacggcggt tggttcgtga
     3181 gcgagttcgc gaacttcgac cggttcctgg caagtgacgc caccctcgtc gaggacatcc
     3241 ccgacgccgc acgccgcctc ggtgcactgg cgccgagcgt ccggccgagt gagcaccggg
     3301 gagcaactcg accatcagcg gtcggcgccc gccgtcaaga cggtcccgtt tgctacgtgc
     3361 cgggggtgtt gatgggagac actgccgtgc gcccgtcgat ctacttcgac gactcgtggt
     3421 accaccgatg gcacctcggg ctcctcgact ggatgcgctc ccggcccgac gtccgcttta
     3481 tctggaaggg cctgccgagt ggcgaccatg tggacgaccc gatcgtggac gccatcgacg
     3541 ccgtccgcaa cgtcgactac gtgacccagc cgctcccgtg gctgctcggg caggtgtcgc
     3601 gggtcgtcac ggacttcccg tcgacacccg cgtacgaggc gctccgggcc ggcctgccga
     3661 ccatcgtgct gtggttcccc cgctttgctg agctgcgtcc cactgcccga gtcgagttcg
     3721 gggagaccgt gcgcgagagc gctgacgaag cggccgccct cgaccacctg tcgaagtggt
     3781 tggtcagctg acgtcgtccc aagagagcgg gtgtcccacc tcgaggtcgc gtgtggcgac
     3841 tcgcccgatg acgtcgggga gatgccgggg ttcgagaccg ccggccggac ggagcgagcg
     3901 gacgtcggtc gatccgacga cctggccggc ggcgagcggc gccacgacgt agagcgagcg
     3961 gcgaaacgcg aggctggccc ggtccgacgc ggacggtccg tagcgaaccg accccagggc
     4021 cgcctcggcg gtccgcaccg acgcgacgag acgggtgagc tcctcgggtt cgagcgagaa
     4081 gctgctgtcg ggaccgccgt ggcctcggtc gagcatgaag tgcttctcga cgatgctcgc
     4141 cccgagggcg acggcgacga cagcgctggt gtcgtcgggg gtgtgatcgg acagaccaac
     4201 cgggacctcc catcggtttg ccaggtcgac gatcgaacga aggtccatgt cggccggtgg
     4261 cgagggatag gcgctgttgc agcggagcag ggccacaccg ccggcaccgg cgttgcgggc
     4321 caccgacacc gcatcgtcga tctcctccac cgtcgccata ccggtcgaca tcacgagcgg
     4381 gaggcccgac gatgcagcgt gggcgatcag cgggaggtcc gtcagctcga aggaggcgat
     4441 cttcagcgcc ggtgcgccct gttcgacgag gaagtcgacc gacgccgggt cgaatgggct
     4501 cgagaacaga gtgatgccct ccgacgcggc caccgcggcg atctcggggt accagtccca
     4561 cggcatcgcc gcttcgcggt agaggtcgtg caaccggcgc ccggaccacg ggttgccggg
     4621 tccgacgacg aaccgaggga ggtcgacatc gagtgtcatc gagtcggcgg tgaaggtctg
     4681 cagcttcacg gcgtctgcgc cggccgccgc cgcgacccgg accatctcga ggacgcgggg
     4741 aaggtcgccg ccgtggttgg cggagacctc ggcgatgacg aacgtcggtt gcccggtgcc
     4801 gaccagacgg ccggcgatct cgatggtggt catgtcgtgc ctgctctgga gcggaagcgg
     4861 agcgccccgt ccatcggatc catcggctcg aaaccggcaa gatcgagcgc ccgaaccgac
     4921 gcacggttgt cgggcttgac caacgcaatc acctcgccgg cggggtgacg ccggaaatgc
     4981 tgtcggcacg ccgccacgag caggggagag gcgagcccgc gcccccggct cgcggggccg
     5041 accaggtagc ccagcgtcgt cggcgtcccc tcaaatcgcg cctgtccgac ggggacgccg
     5101 tcggactcga cgatgaggaa gtcgccctcg ggatcggcga gccgccgagc gaaccactcc
     5161 tcgtgtcggt gtcggggaat gctcgccgat tcgaaggatt ggcgacgcac gtcgggatcg
     5221 ttcgaccact cccaggtcag atcgaggtcc tcgggagtcg ccgggcggac gtcgaggagg
     5281 aacgagcgca tggccgtcac gacgcggtcc gctccgagcc cgtcgaccag tcgccgcgcg
     5341 acacgggtca tcgtggcgca gcggtcagga tcatcgagca gcccgagggc ggtctccacc
     5401 gtgcggtcca cgctgtcggc gtggtcggcc gacacggcgg cgccggcgtc gtcgagtcgt
     5461 cgagcgaccg gctcctggtt ctcggcgacg gcgacgagga ctgccggcac gccgactcgg
     5521 cacaactcgt acgcggtgac cccggctgcg gcgacggcga cgtccgcgga cgccatcgtc
     5581 gccacgacat cgtcgacacc tcgcagccac accaggccgg cgccggctgc gatcagaccg
     5641 gcggcgatgc catcgaacgt cgctgtggtc gacgacgagg gggccccgcc gggagcgagg
     5701 agcactcgcc ggcccggtgg gggtcgatcc ggtggttcgt cggcgaactc cgtgcggagc
     5761 aggagatagc gggagccgag caggtcgcca cggtcggccc cgatgttgtg gtccagcacg
     5821 aggtcggcgt cgtaggcgcc cagtgatccg tggtcgtcga tcacgagcac gtggt
//
LOCUS       DAOSOA010000205         5872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13739_length_5872_cov_3.836857, whole genome shotgun sequence.
ACCESSION   DAOSOA010000205 DAOSOA010000000
VERSION     DAOSOA010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13739_length_5872_cov_3.836857"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1107
                     /locus_tag="PKY13_11955"
     CDS             94..1107
                     /locus_tag="PKY13_11955"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT80697.1"
                     /translation="MSMDGDAGKDATQRGDADELRAQQIDYFARCFAHLSEHVGAVVL
                     GASRAIELALTCLFSEGHLLLEDLPGVGKTTLARVISASIDARHDRVQFTPDLLPSDV
                     TGFSVFHPGTQEFEFREGPVFANIVIADEINRASPKVQSALLEVMEERRVTVDGATHP
                     VPRPFMIVATQNPIELEGTYRLPEAQLDRFLIRTSMGQPSLEAEVAMVLAQGVVASET
                     APSGLRSDHIEALIAIAAAVRVREPVVEYAASICRATRTGPNVRMGASPRATLALVRA
                     ARVRAAADRRDFVSPDDIRSLTRPVLAHRIELHASASVHKITQDDIIDEAVRSIAPPQ
                     DRG"
     gene            1111..2256
                     /locus_tag="PKY13_11960"
     CDS             1111..2256
                     /locus_tag="PKY13_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT80698.1"
                     /translation="MPTRRGFGLVAVAATLTVLAAFGGLPSVAVLGVGGLALVAVSGL
                     ATLSPVRLDVRSIDVPASRVEVGTRGQITIVLTNPSARRAPALTLFLIALDDRITVAV
                     PRLAPGADVTVTLPLPTKRRGSGSIGPLVTDSADPFGLFRRRLEFGRPQEFIVHPRHH
                     RLEVIAPSSIRNSEGPAGPYRRPDAASFVGLRVYRPGDDRRQIHWPTVARTGELMVRE
                     HADATLERLVLAFDTRSARWDEVRFEQAVEVVASVAVAVTGSGVPLTLVIGPDRHDVG
                     GRDAAAGVVLDLLAGVAIGPAAPKGPTALDTLLTLTSATTSVLVTGAADAGDHVALAE
                     LATRLPVVVIAEIASALAPRSAPASTRVRSVRAATSSEFARLWDVLR"
     gene            2253..4481
                     /locus_tag="PKY13_11965"
     CDS             2253..4481
                     /locus_tag="PKY13_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOT80699.1"
                     /translation="MRSPTGGRDIELVASTLCVGAAATTFVGTFVPLRLVVPIVVGST
                     MPGAIAVWSGRRRPESPVWAVAAAQAGAFVVAWVALFVRTVPTVSGLPGAFWRALRDG
                     GAEVLSTVTPVVADRNTLTVPFLLVWGAAALSSAAVLSSRAAPLVIAPAGVLAAAGVL
                     VSWRGALPYRAWPVVMLVAAGAVFVATARRRALGRGWPGASLVAAAVALAVPIAGLGL
                     FRTGGPAVLRERWQPASSSFAPEHPFAVLARARRADGSSGVVADVSIRGPVARPVVMR
                     LATLDAFDGVRWRSTGRYAPLGEQLPPLSDRNDAPIGGTAVDAVITFHAGNRLPFVPQ
                     LGTYAALRSDASVPQLVMNPRDGSLAFRSGRPAQAGTMRADAVVPDGSVLTDGVPSLV
                     DDSEPDAIVDVPGLADVAGGGPGDPRSTVDAITRLLGNENGESPRFAVDPTIEGPITV
                     ETIRVFLGLDGDRPEVGFGPPEEFAVAFALLAHERDVAVRLGYGFVLSPDEVRDGSVS
                     LTEAMLSVWPEVHVAGAGWVRVDPVPDRHAVHDADVVATTTSTTTTTTTTTTIATAAD
                     PTPDVPQGEAGSAGAAGSSGAAQHDTGGRSVWLILLVVAVLLSTAGAGLPRFLKMRRR
                     ALRQRGSPAARVAGAWSELRDELGRRQIPRPRWLTTSDTVAAVRRSMGDEAAVEAANL
                     AGVLDLSLFGQDDPTDADAIAAWALVSRVKAVARRPERRSARMRIVDPISRLGHRSSR
                     GG"
     gene            complement(5359..>5872)
                     /locus_tag="PKY13_11970"
     CDS             complement(5359..>5872)
                     /locus_tag="PKY13_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT80700.1"
                     /translation="HYDAPKGTVTIEEAIDKRGRRKDPKTFASRRTVALPRFVLDALD
                     DHVETYVDGSPDSPLFPGEAGGVMSDGWFKKSWTAARKSVGVSTVRFHDLRHTAGTLA
                     TQHGATMKEVMSRLGHSTTAAAIRYQRAADSRDQDVADRLDEAFGNPTSEPVAGYSRD
                     EPDEGGEPDS"
BASE COUNT          747 a         2034 c         2074 g         1017 t
ORIGIN      
        1 caactggtgg atgatcgcgc ggagcgcgcg aatcccttgg aactactcat ctagggcgag
       61 ccgcactaac ctgactcgcc ctgggaggga ccgatgtcta tggatggtga cgcgggcaag
      121 gacgccacgc agcgcggcga tgccgatgag ctccgtgcgc agcagatcga ctacttcgct
      181 cgttgtttcg cccacctctc cgaacatgtc ggggccgtgg tattgggtgc ttctcgtgcg
      241 atcgagttgg cgctcacgtg cctgttcagt gagggtcatt tgctcttgga agacctgccg
      301 ggagtgggca agacgaccct ggcgcgagtg atctccgcct cgatcgacgc ccgccacgac
      361 cgtgtgcagt tcacgccgga cctgctgccg tccgacgtga cggggttcag tgtcttccac
      421 cccggcaccc aggagttcga gttccgcgag gggccggtgt tcgcgaacat cgtcatcgcc
      481 gacgagatca accgagcctc gcccaaggtt cagtccgcac tcctcgaggt gatggaggag
      541 cgtcgcgtca ccgtggacgg agcgacccac cccgtcccgc gacccttcat gatcgtcgcc
      601 actcagaatc ccatcgagct cgaaggcacg tatcgtctcc ccgaggctca gctcgaccgg
      661 ttcctcatcc ggacgtcgat gggacagccg tcgctcgagg ccgaggtcgc gatggtgctc
      721 gcccagggcg tggtcgcgtc tgagaccgcg ccgtcggggc tgaggagcga ccacatcgag
      781 gccttgatcg ccatcgctgc ggcggtgcgc gtccgtgagc cggtcgtgga gtacgccgca
      841 tcgatctgtc gcgccacccg cacgggcccg aacgtccgca tgggggcgag cccacgggcg
      901 acgctcgcgc tcgtgcgtgc cgcccgggtg cgcgccgccg ccgaccgacg agatttcgtc
      961 tcgcccgacg acatccgttc gttgacacgg cccgtgctcg cccaccggat cgagctccac
     1021 gcgtcggctt cggtgcacaa gatcacccag gacgacatca tcgacgaagc ggtgcgctcc
     1081 atcgcacccc ctcaagaccg gggctgaacc atgccgaccc gtcggggctt cgggctggtc
     1141 gccgttgccg cgaccctgac ggtcctcgcg gcgttcggcg gcttgccgtc cgtcgccgtg
     1201 ctcggtgtcg gtggcctcgc gctggtcgcg gtgagcggcc tggccacgtt gagcccggtc
     1261 cgtctcgacg tccgttcgat cgacgtacct gccagccgcg tcgaggtggg gacgcgcggc
     1321 cagatcacga tcgtcctgac caacccgtcg gcgcgccgtg ctccggccct caccctcttc
     1381 ctcatcgccc tcgacgaccg gatcacggtc gccgtgcccc gactggcgcc gggagccgat
     1441 gtcacggtga cgctcccgct gccgacgaaa cgccgggggt caggctcgat cggcccgttg
     1501 gtcaccgact cggctgatcc gttcggactc ttccggcgcc gactcgagtt cggtcggccg
     1561 caggagttca tcgtccaccc gcgtcaccat cgcctcgagg tgatcgcgcc ctcgtcgatc
     1621 cgcaactccg aggggccggc aggcccgtac cggcgccccg acgccgcgtc gttcgtcgga
     1681 ttgcgggtct accgacccgg cgacgatcgg cgccagatcc actggccgac ggtcgcccgt
     1741 accggggagc tcatggtgcg cgagcatgcc gatgcgacgc tcgaacggct ggtgttggcg
     1801 ttcgacacca ggtcggctcg gtgggatgag gtgcggttcg agcaggccgt cgaagtggtg
     1861 gcgtcggtgg cggtcgccgt caccggctcg ggtgttcccc tcaccctggt gatcggcccg
     1921 gatcgtcatg atgtcggtgg ccgcgatgcc gccgccgggg tcgtcctgga tctcctggcc
     1981 ggtgtggcga tcggacccgc cgcgccgaag ggtcccaccg ctctcgacac cctgctgacg
     2041 ctcacctcgg cgaccaccag cgtgctcgtg accggtgcgg cggacgccgg ggaccacgtc
     2101 gcactcgccg aacttgcgac gcgtctcccc gtggtcgtga tcgccgagat cgcgtcggca
     2161 ctcgccccac gctcggcgcc ggcgtcgacg agggtgcggt cggtgcgtgc cgccacctcg
     2221 agcgagttcg cccgcctctg ggatgtgctg cggtgaggtc gccgaccggc ggccgggaca
     2281 tcgagctggt ggcctcgacc ctgtgcgtcg gagcagccgc gacgacgttc gtggggacct
     2341 tcgtgccgct gcgtctggtc gtcccgatcg tcgtgggctc gacgatgccc ggggcgatcg
     2401 ccgtctggtc gggacgccgg cgacccgagt cgccggtgtg ggcggtggcc gcagcgcagg
     2461 ccggggcgtt cgtcgtggcg tgggtggcgc tgttcgtccg cacggtaccg accgtctcgg
     2521 gcctccccgg agcgttctgg cgagcccttc gtgacggtgg cgcggaggtg ttgtcgaccg
     2581 tgacacccgt cgtcgccgac cggaacacgc tgaccgtgcc gttcctgctc gtgtggggag
     2641 ctgcggccct gtcgtcggcg gcggtgctga gctcacgtgc tgccccgttg gtgatcgccc
     2701 cagccggcgt gctggcggcg gctggcgtgc tcgtgtcctg gcgtggcgcc ttgccctacc
     2761 gggcctggcc cgtggtcatg ttggtcgctg cgggggcggt gttcgtggcg accgcgcgtc
     2821 ggcgcgccct cggccggggg tggccgggag cctcgctggt tgcggccgcc gtcgctctcg
     2881 ctgtgccgat cgccggactc gggctgttcc ggacgggtgg gccggccgtg ttgcgcgagc
     2941 ggtggcagcc ggcgtcgtcg tccttcgctc ccgagcaccc gttcgccgtg ctggcgcggg
     3001 cgcgacgggc cgacggatcg tcgggcgtgg ttgccgacgt gtcgatccgg ggtccggtcg
     3061 cacgaccggt ggtgatgcgc ctcgccaccc tcgacgcctt cgacggcgtc cgatggcgtt
     3121 cgaccggacg gtacgcgccc ctgggcgagc agctcccgcc gttgtccgac cgaaacgacg
     3181 caccgatcgg gggcactgcc gtcgacgctg tgatcacgtt ccatgccggc aaccgcctcc
     3241 ccttcgttcc ccaactcggg acctacgcgg cgttgcggtc cgacgcgtcg gtgccgcaac
     3301 tggtgatgaa cccacgcgac gggtcgctgg cgttccgttc cggccgaccg gcgcaggcgg
     3361 gaacgatgcg agccgacgcg gtcgtgcccg atgggtcggt cctgaccgat ggtgtcccgt
     3421 cgctcgtcga cgactccgaa cccgatgcca tcgtcgacgt gcccggtctc gccgacgtgg
     3481 ccggcggcgg cccgggcgac ccccgcagca ccgtggacgc catcacccga ctcctgggca
     3541 acgagaatgg cgagtcacct cggttcgccg tcgatccgac gatcgagggc ccgatcaccg
     3601 tcgagaccat tcgggtgttc ctcggcctgg acggcgatcg tcccgaggtc ggcttcggtc
     3661 ctccggagga gttcgcggtg gcgttcgcgc tccttgctca cgagcgcgac gttgcggtcc
     3721 ggctgggcta cggattcgtg ctgtcgccgg acgaggtgcg ggacgggtcg gtctcgctga
     3781 ccgaggcgat gctgtcggtg tggccggagg ttcacgtggc cggagccggt tgggtgcgcg
     3841 tcgatccggt tcccgatcgt cacgccgttc acgatgccga tgtcgttgcg acgacgacgt
     3901 ccacaacgac gaccaccacg acgaccacga ccatcgcaac ggccgccgac ccgacgcccg
     3961 acgtgccaca gggtgaggcg ggatcggcgg gtgcggcagg ctcgtcgggc gcagcgcagc
     4021 acgacaccgg gggtcggagc gtctggttga tcttgctcgt cgtggccgtg ctgctctcga
     4081 ccgccggtgc cggtctcccg cgtttcctga agatgcgtcg gcgtgcgctg cggcagcggg
     4141 ggagcccggc ggcccgggtg gcgggggcgt ggagcgagtt gcgcgacgag ctcggtcgtc
     4201 ggcagatccc gcgcccgcgg tggttgacga cgtccgacac cgtggccgcc gtgcggcggt
     4261 cgatgggaga tgaggcagcg gtcgaggcgg cgaatctggc tggcgtgctc gatctcagcc
     4321 tcttcgggca ggacgacccg accgatgcgg acgccatcgc ggcatgggcg ctcgtcagtc
     4381 gtgtgaaggc ggtcgcacgc aggccggagc ggcggtcggc gcggatgcgg atcgtcgacc
     4441 cgatcagccg cctcggacat cgatcatcgc ggggcggctg accgccgagg actcgttgcg
     4501 gggcaaggcg gtcgaccacc gcgaccatcc atggcacctg tggtcgaact gctaccggac
     4561 acgcgccgct ggtccctgtc ttgtctgtcg agccgcggtc ggactggcgc ctcttgggga
     4621 actcagcggt cgctctctgc ggaacatggt ccggggtcgt gttgcggtct cgcgctccat
     4681 tggacggtca ccctcgccgc tccgacggtc gaggtgttcg ccgaggttcg cagccacttc
     4741 gtgggccgat cgaggatccg gcctggatcg gccggccgcc gccggtagcg tgatcggcgt
     4801 ggctaccgag cgctacgacc tctggttcga cccgaactct gtcggcggag acatggtcgt
     4861 cgagtgctgg atcggccatg gcccacatgc aaacgaccct gacttcgccc cggttccagg
     4921 tgactggctc gtgcttggcg atggtgacga ggccccgctc cgtgcccgag tgatcaagcg
     4981 ccagggcgac cgtgtgtcgg tgcagatcca gctgggtgcc gaaggcgcct cggccgcctg
     5041 ccgcctccca cgattccgtc ggagtatcga cgtgtcgtcg cctcctgggg cggtctggtg
     5101 tcgccacgcg tcaggagact gggtggcaat gacttgaccg aacgacgggc gtgggcccac
     5161 accgataccg cggaaatccc gcggccgccc tgaatcgcac cggccgctca cggccgccac
     5221 gtccagaaaa ccccaagatc tcgcccgttc cgtccgctga cgttcgctgc ggccacgtcg
     5281 ggaatccctc tcaaggtggc ggcacgggtt cgaatcccgt tgggggtaca cctccaaagc
     5341 cccgaaaact cgggactttc aggagtcggg ctcaccaccc tcatccggtt catcccgcga
     5401 atatcccgcg acaggttctg aagtggggtt tccgaacgcc tcatcgagtc gatctgcgac
     5461 gtcctgatcc cgcgaatccg ccgctcgttg gtatcgaatc gcggcagcag tcgtcgagtg
     5521 acccagccgc gacatgacct ccttcatcgt cgcgccgtgt tgggtggcga gcgtgccggc
     5581 ggtgtgccgc aggtcgtgga accggacggt cgacaccccg acggacttcc gggcggctgt
     5641 ccagctcttc ttgaaccagc cgtcgctcat gacgccgccg gcctcgccag ggaacagcgg
     5701 gctgtccggt gaaccgtcca cgtaggtctc gacgtgatcg tcgagcgcgt ccaacacgaa
     5761 ccgggggagg gcgactgttc ggcggcttgc gaacgtcttc ggatccttcc gacgtccgcg
     5821 cttgtcgatc gcctcctcga tcgtgacggt gcccttcggc gcgtcgtagt gg
//
LOCUS       DAOSOA010000206         5820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13893_length_5820_cov_5.794970, whole genome shotgun sequence.
ACCESSION   DAOSOA010000206 DAOSOA010000000
VERSION     DAOSOA010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13893_length_5820_cov_5.794970"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1712)
                     /locus_tag="PKY13_11975"
     CDS             complement(102..1712)
                     /locus_tag="PKY13_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT80701.1"
                     /translation="MSAVAEPIDDVAVDLDGYGRSGEPGERAEIGTPYVRPARLAIFF
                     GVFGALALWRGLPIMFVVLAVLVMIFLHELGHYVMARRAGMKVTEFMIGFGPRIFSFR
                     RGDVEYGIKAIPAGAYVKIIGMANIEEVPAADESQTYRQKGYWARMGVAVAGSTMHFI
                     LALLLIGASFVFVGKMSDTQWAVDTIAPDSAAALAGIRDGDKVVAVDGVKVATHDAMA
                     VQAKKHVGETIPVVVERDGKEVELTASITPRFSVFGTIGEEFQIYGNADGGFSLSLAQ
                     VNGLDGLRDGDVLTSVDGTEVTSLDQLRSLLSAPAVATTGNMVLGVVHEGASRPVDVK
                     VDLGTAVGLDKTNGFFGVGQERIPDKMGVLEAVPETFKWFGETTKLSVVGMAKFFNPS
                     SLVDFAKRTFTTVPGETADTGLPQRNSDAATQNVERNSNRIVSIVGAVVLGEELTDGG
                     WANLLAFFAFLNMAIGLLNLIPLPPFDGGHVAVGTYEKIRELLRRDGRRYFADYNKVM
                     PVAMAVVSFMVIVGLMAMYLDLADPIRL"
     gene            complement(1709..2881)
                     /gene="dxr"
                     /locus_tag="PKY13_11980"
     CDS             complement(1709..2881)
                     /gene="dxr"
                     /locus_tag="PKY13_11980"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOT80702.1"
                     /translation="MTSLAIMGSTGSIGTQTLEVLDTAPGRFDVDSLGANGGNLELLA
                     AQARAVHPRTVAIADSSRAAELKELLPSGVHLEAGPDALADVCASADVVVNGVVGFAG
                     LGVTMATLSAGHRLALANKESLIAAGPIVQPLRATPGAELIPVDSEHCAVHQCLRSGA
                     DGPGGRRLRSVVLTASGGPFRGRTRADLESVTVEQALAHPTWAMGPKITVDSSTLVNK
                     GLEVIEAHELFGVGYDDIGVVVHPTSIVHSMASFTDGATIAQLSLPDMRLPIGYALGW
                     PDRFETAFGAMDFSQPFDLHFEPPDHDAFPCLGLAYTAGRLGQTAPAWFSAANEIAVE
                     AFLAGRIRWVDIADVNADALERWDGTAADTIEAVLAADAHARVVAADLVSMYEEAR"
     gene            complement(3021..4574)
                     /locus_tag="PKY13_11985"
     CDS             complement(3021..4574)
                     /locus_tag="PKY13_11985"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOT80703.1"
                     /translation="MADEPFDDEGRTPTPGPRTQPEGVRIIGAQEAAELVSRADDDRG
                     ADPTEAMPIPTASDADDERPALRFPLPDEPVEPAAPSAVVDPEQSFELPHYSDPPTGQ
                     VPKVVIGEETDSRWSGLADQPRWRDTEHHFDEQGGFEHLVDDSPRLGALGETGEHRDF
                     FADLDLTDDGVPTLAGAPSREERAVTAAVEPEVAEEAAPRRPPGPRRRRPAGAGPTGP
                     VSGRSTALGADAGNGARNLPMAAAVGVGLIAIGGGCFYLGGFATMILITVVLCLCAAE
                     FFTSLSQSGYRPAGLLGVVAVAGLSIAPLYYGFFAYPIVLGLTMLTGLAWFLFVQPGE
                     GAVMNLGVTLLGIGYIGVLGSFATLMVGVGRIVETDATSNQGIGVLVAAVLITVSYDV
                     GAYFIGKSFGSTPLTQVSPNKTQEGLIGGVAAAILIPFLILWLSEWAPVGVDWATAIR
                     FCLICAVMAPVGDLCESAIKRDLGVKDMGTLLPGHGGVLDRFDALLFVLPTAYFMAHL
                     LELGKPAFF"
     gene            complement(4574..5143)
                     /gene="frr"
                     /locus_tag="PKY13_11990"
     CDS             complement(4574..5143)
                     /gene="frr"
                     /locus_tag="PKY13_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT80704.1"
                     /translation="MSEELIEAVREDSEERMTKAVAYTRSEFAGIRSGRANSGLVERL
                     PVEYYGSTVPLQQLASFSVPEARMLVISPFDKASIGAIERSIREANIGLNPSNDGVQI
                     RLGFPSLTEERRKEFVKMVRSKAEDGKTAVRGARRDARKDLEALQKDGDITEDQLRRS
                     EEELDKMTKRHEAEIDAALESKTVELMEG"
     gene            complement(5285..>5820)
                     /locus_tag="PKY13_11995"
     CDS             complement(5285..>5820)
                     /locus_tag="PKY13_11995"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HOT80705.1"
                     /translation="TGAGAGMDRAQADYMGMLATVMNALALQETLERLGQPTRVQSAI
                     HMSQVAEPYIRRRAIRHLEKGRVVIFAGGTGNPFFTTDTTAALRAVEIDAEAILMGKN
                     GTDGVYSDDPRTNPDAEKLDTVSYIDVLSRGLKVMDSTAISLCMDNELPIVVFDLMGD
                     RLISRILSGDTIGTLVS"
BASE COUNT          989 a         1948 c         2029 g          854 t
ORIGIN      
        1 cgagacgggg gctcccccgc cgacggcaac cgtgcccacc atcacctggc gggtctcgcg
       61 acggggaaag gtcagtgctc cggcatccat caggtccctg tctacagccg aatcgggtcg
      121 gccaggtcca agtacatcgc catgagaccg acgatcacca tgaacgacac gacggccatc
      181 gccaccggca tgaccttgtt gtaatcggcg aagtaccggc gaccgtcccg tcgcagcagc
      241 tcgcgaatct tctcgtacgt gccgaccgca acgtgcccac cgtcgaacgg cgggagcgga
      301 atcagattga gcaggccgat ggccatgttc aggaaggcga agaacgccag caggttcgcc
      361 cacccgccat cggtgagctc ctcaccgagc acgacggcac cgacaatgga cacgatgcgg
      421 ttgctgttgc gctcgacgtt ctgggtcgcg gcgtcggagt tgcgctgtgg gaggccggtg
      481 tcggccgtct cgccgggaac cgtcgtgaag gtgcgcttgg cgaagtcgac caggctggac
      541 gggttgaaga acttggccat gccgacgacc gacagcttcg tggtctcacc gaaccacttg
      601 aaggtctcgg gcaccgcctc gagcacaccc atcttgtcgg ggatgcgctc ctggcccacc
      661 ccgaagaacc cgttggtctt gtcgagacca acggcggtgc cgaggtcgac cttcacgtcg
      721 acggggcgac tggcaccctc gtggaccacg ccgagcacca tgttgcccgt ggtggcgacg
      781 gccggagccg acaggagcga acggagctgg tcgagcgacg tcacctcggt gccgtcgacc
      841 gacgtgagca cgtcgccgtc gcgaaggccg tcgagaccgt tgacctgggc cagcgagagc
      901 gagaacccac cgtcggcgtt gccgtagatc tggaactcct cgccgatcgt gccgaacacc
      961 gagaacctcg gggtgatcga ggccgtcagc tcgacctcct tgccgtcgcg ctcgaccacg
     1021 accgggatgg tctcgccgac gtgcttcttg gcctggacgg ccatcgcatc gtgggtggcg
     1081 accttcacgc cgtcgaccgc cacgaccttg tcaccgtcac ggatcccggc caacgcggcg
     1141 gcgctgtcgg gcgcgatcgt gtcgaccgcc cactgggtat cagacatctt gccgacgaag
     1201 acgaacgagg caccgatcag cagcagcgcc aggatgaagt gcatggtcga tccggcgacg
     1261 gcgacaccca tgcgggccca gtagcccttc tggcggtacg tctgggactc gtccgcggcc
     1321 ggcacctcct cgatgttcgc catcccgatg atcttcacgt aggcgccggc ggggatggcc
     1381 ttgatgccgt actcgacgtc accacggcgg aacgagaaga tccgcggccc gaacccgatc
     1441 atgaactcgg tgaccttcat gccggcccgg cgagccatca cgtagtggcc gagttcgtgg
     1501 aggaagatca tgaccaggac cgccaggacg acgaacatga tcggcaggcc ccgccacagg
     1561 gcgagcgccc cgaacacccc gaagaagatc gccagacggg cgggacggac gtagggcgtg
     1621 ccgatctcgg cgcgctcgcc cggctcgccc gagcgtccgt agccgtcgag atcgaccgcg
     1681 acgtcgtcga tcggctctgc cactgcgctc atcgagcctc ctcgtacatg ctcaccagat
     1741 cggcggcgac gacccgagca tgagcatcgg cggccaggac cgcctcgatc gtgtcggcgg
     1801 ctgtgccatc ccaccgctcc agcgcgtcgg cgttcacgtc tgcgatgtcg acccaccgga
     1861 tgcgtccggc gaggaaggcc tcgacggcga tctcgttggc cgccgagaac caggccggcg
     1921 ccgtctgacc gagccgtccg gcggtgtacg ccagcccgag gcacggaaac gcgtcgtggt
     1981 cgggcggttc gaagtggaga tcgaacggct gggagaagtc catcgcgccg aaggccgtct
     2041 cgaagcggtc gggccacccg agggcgtagc cgatcggcag gcgcatgtcg ggcagcgaga
     2101 gctgggcgat cgtcgcgccg tcggtgaagc tggccatcga gtgcacgatc gacgtggggt
     2161 gcaccacgac cccgatgtcg tcgtacccga cgccgaacag ctcgtgggcc tcgatgacct
     2221 cgaggccctt gttgacgagc gtcgacgaat cgaccgtgat cttggggccc atcgcccacg
     2281 tcgggtgcgc cagcgcctgc tcgacggtga ccgactccag atcagcgcgg gtgcgaccgc
     2341 ggaacggacc gccggacgcg gtgagcacga ccgaacggag gcgacgaccg ccgggaccgt
     2401 cggcgccgga gcggaggcac tggtggacgg cgcagtgctc ggagtcgacc gggatcagct
     2461 cggcccccgg cgtggcccgc aacggctgca cgatcgggcc cgccgcgatg agcgactcct
     2521 tgttggcgag ggcgagccgg tgaccggccg agagcgtcgc catcgtcaca ccgagaccgg
     2581 cgaacccgac gacaccgttg acgaccacgt ccgccgacgc acacacgtcg gcgagggcat
     2641 cggggccggc ttcgaggtga acaccactcg ggaggagctc cttgagctcc gcggcccggg
     2701 acgagtcggc gatcgcgacc gtgcggggat gcacggcgcg tgcctgggcg gcgagtagtt
     2761 cgagattgcc gccgttggcg ccgagcgagt cgacgtcgaa ccggcccgga gcggtgtcga
     2821 gcacctcgag ggtctgggtg ccgatggacc ccgtggagcc catgatcgcg agtgaggtca
     2881 tgacggagct gagcgtactg cgaccccaac cgacaccggg gtcaaccgcc tgagtgccag
     2941 ctcacccgcc cgtcgggggt tttgtagtgc gaggagcgac gctcgcgtcg ctcctcgcag
     3001 cacaaaaccc ctgggatcgg tcagaagaag gcgggcttgc cgagctcgag caggtgggcc
     3061 atgaagtagg ccgtcggcag gacgaacagc agggcgtcga accggtcgag cactcccccg
     3121 tgacccggga gcagcgtgcc catgtccttg acgccgagat cgcgcttgat ggccgactcg
     3181 cacaggtcgc ccacgggcgc catcacggcg cagatgagac agaaccggat ggcggtcgcc
     3241 cagtcgacac cgaccggcgc ccactccgac agccacagga tgaggaacgg gatcaggatc
     3301 gctgcggcga caccgccgat caggccttcc tgggtcttgt tcgggctgac ctgcgtgagc
     3361 ggtgtgctcc cgaaactctt gccgatgaag tacgccccga cgtcgtagct gaccgtgatg
     3421 agcacagctg cgacgagcac gccgattccc tgattggagg tggcgtcggt ctcgacgata
     3481 cggccgaccc cgaccatgag ggtcgcgaac gaaccgagca cgccgatgta gccgatgccg
     3541 agcagcgtga cgccgaggtt catcaccgcg ccctcgccgg gttggacgaa gaggaaccac
     3601 gcgagacccg tcagcatggt gagacccagc acgatcggat aggcgaagaa gccgtagtac
     3661 agcggggcga tcgacaggcc ggcgaccgca acgacgccga ggagcccggc cggccgataa
     3721 ccgctctggc tcagggacgt gaagaactcg gccgcgcaga ggcagagcac cacggtgatc
     3781 aggatcatgg tggcgaaacc accgagatag aagcacccgc cgccgatggc gatgagaccg
     3841 accccgacgg ccgctgccat cgggaggttg cgggccccgt tgccggcgtc ggcaccgagc
     3901 gccgtcgaac ggcccgacac cggaccggtc gggccggcgc cggccggtcg gcggcggcgt
     3961 gggcccgggg gtcggcgcgg cgcggcctcc tcggcgacct cgggctcgac cgcagcggtc
     4021 acggcgcgct cctcacgtga gggggcaccg gcaagggtcg ggacaccgtc gtcggtcagg
     4081 tcgaggtcgg cgaagaagtc acggtgctcg cccgtctcgc cgagggcacc gaggcgcggc
     4141 gaatcgtcca cgagatgctc gaacccgccc tgctcgtcga agtggtgctc ggtgtcgcgc
     4201 cagcggggct ggtcggccag accggaccag cgggaatcgg tctcctcgcc gatcacgacc
     4261 ttcgggacct ggcccgttgg cgggtcggag tagtgcggga gctcgaagga ctgttcggga
     4321 tcgacgaccg cggacggcgc cgcgggctcg accggctcgt cggggagcgg gaaccgcaac
     4381 gcgggacgct cgtcatcggc gtcggacgcc gtcgggatcg gcatcgcctc ggtcggatcc
     4441 gcaccacggt cgtcgtcggc gcgcgacacg agctcggcgg cctcctgggc accgatgatg
     4501 cggacgccct cgggttgggt gcgaggaccg ggcgtcggtg tacggccctc gtcgtcgaac
     4561 ggttcgtcgg ccatcagccc tccatcagtt cgacggtctt ggattcgagc gcagcgtcga
     4621 tctcggcttc gtgccgcttc gtcatcttgt cgagttcctc ctcggaacgc cgcagctgat
     4681 cctcggtgat gtcgccgtcc ttctggagcg cctcgagatc cttgcgggcg tcccgacggg
     4741 caccacggac ggcggtcttg ccgtcctcgg ccttgctccg caccatcttc acgaactcct
     4801 tgcgtcgctc ctcggtgagc gacggaaacc ccaggcggat ctgcacaccg tcgttggacg
     4861 ggttcagacc gatgttcgcc tcacggatcg aacgttcgat ggcaccgatg gacgccttgt
     4921 cgaagggact gatgaccagc atgcgggcct cgggaaccga gaaggacgcc agctgctgca
     4981 gtggcaccgt cgagccgtag tactccaccg gcaaccgctc gaccaggccg gagttcgcac
     5041 ggccgctgcg aatgccggcg aactccgaac gggtgtacgc gaccgccttg gtcatgcgtt
     5101 cctcggagtc ctcgcggacg gcctcgatca actcttcact catggcggac agcctgacac
     5161 aacatcgcgc gcggggcgcg acaacggggg gacgaagtgg cacctccgta catcggaaca
     5221 accgaggtat cggagtggga ggcgcgggtg cggcgacgcc gcggagcgca ggagccgctc
     5281 gccgctagct gaccaaggta ccgatggtgt ccccggacag gatgcggctg atgagccggt
     5341 cgcccatcag gtcgaacacg acgatcggca gctcgttgtc catgcacagg ctgatcgccg
     5401 tggagtccat caccttgagg ccacggctca gcacatcgat gtagctgacg gtgtcgagct
     5461 tctcggcatc gggattggtg cgagggtcgt cggagtagac cccgtcggtc ccgttcttgc
     5521 ccatcaggat cgcctcggcg tcgatctcga cggcccggag cgcagcggtg gtgtccgtcg
     5581 tgaagaacgg gttgcccgtc ccaccggcga agatgacgac ccggcccttc tcgaggtgcc
     5641 ggatggcccg acggcggatg tacggctcgg ccacctggga catgtggatc gcggactgca
     5701 cgcgggtcgg ctggcccaac cgctcgagcg tctcctgcag ggccagggcg ttcatgaccg
     5761 tggcgagcat gcccatgtag tcggcctggg cgcggtccat gccggcaccg gcgccggtca
//
LOCUS       DAOSOA010000207         5820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13894_length_5820_cov_4.737901, whole genome shotgun sequence.
ACCESSION   DAOSOA010000207 DAOSOA010000000
VERSION     DAOSOA010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13894_length_5820_cov_4.737901"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..737)
                     /locus_tag="PKY13_12000"
     CDS             complement(165..737)
                     /locus_tag="PKY13_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT80706.1"
                     /translation="MPNRTPRERIMDAVMVCVDRGGLHSFALEDVAAEANLSRATIYR
                     HFPGGRTQLINDTVTREVARFWGELADHVRPLAAIEDRLVVGLMEARRRIDEYDLLQR
                     LVASEPDDLLPALSESDQLVQLAIMGYIKDLISREQLVDGLDLETAAEYLTRMLLSHI
                     GTAGRWDLTDRTQVERLVRTQFLAGIIQRQ"
     gene            complement(746..1576)
                     /locus_tag="PKY13_12005"
     CDS             complement(746..1576)
                     /locus_tag="PKY13_12005"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOT80707.1"
                     /translation="MYRFLYKPKWIVSHVLILGLIVSMILLMFWQLRRLDEKQTLNAR
                     IAARADGAPTQLADVLRDERVSTIADGDRVEYRAVVVDGTYDVAAEFTVPNRTLDGAP
                     GRMVVTPLRWSDTEAPILVLRGFIPQAIEDNTAPIEGAEPPTGPVQVRGWLRVSELPG
                     SFQSSKVDLGSNRIARLDIDRVKAARDLDLQPVYLELGGQAPPTDGVIKPYPLPERSE
                     GPHFAYAVQWGVFTLIAIGGYPLVIRRVARGGGKDRKDDVPTDPDDLDAGGVPVAAGT
                     "
     gene            1689..2387
                     /locus_tag="PKY13_12010"
     CDS             1689..2387
                     /locus_tag="PKY13_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80708.1"
                     /translation="MTAEDVTLETAPRRKPPSWVLPTALVPMIAVLVCGWVAGSVWAN
                     WQVNHPLGLIALSPINRFLLLTTNRLDFWSYFSVGLGRHLFPDPFFYLLGYWYGGRAL
                     KWVSEGNAFATRLVGGDGHGLENPAHRKVLYPLAFVMPNNWVSLFCGAARIPIPAFIA
                     LNISGTLARLVLCRWLGDLFRSEIKDISDFIARFQWPITAVSVVIVLLGMAVQLRPNG
                     AVRRLAALDDQPEP"
     gene            complement(2451..3293)
                     /locus_tag="PKY13_12015"
     CDS             complement(2451..3293)
                     /locus_tag="PKY13_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT80709.1"
                     /translation="MWVTLAVLVAGFGSGVLAAVLGVGGAVITIPMIRALGASPIAAV
                     GSTVPAILPGALSGSLRFHREGYIVWPVALTCGVSGMSFAVLGGWVADIVDARWLMIL
                     TAVLVMWCGVSLLRDGLRTNAAERADAAVEAGADPDDLVVSDGGAGTEGGVAIQPRGG
                     VPAMVALGVGGGFLAGLLGIGGGLILTPGLTLGLRLPVKHAIATSLAAVAMMSTTAVV
                     THIVLGHVNWRFALPLAIGIVPGARVGAHITVGASEQLMRLVAGSLLIVVSIIYLAGE
                     VAAL"
     assembly_gap    3398..3497
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3498..4266)
                     /gene="dapB"
                     /locus_tag="PKY13_12020"
     CDS             complement(<3498..4266)
                     /gene="dapB"
                     /locus_tag="PKY13_12020"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOT80710.1"
                     /translation="MVESQRKIRVGVFGAGGRMGSTVCRAVHDDPALELVAAVDPHHS
                     GLEVSRVAGVPGCDVQISGNPDVFVEANVQVAIDFTEADAGRQNLIFLAQHGIHAVVG
                     TTGFSDDDLDLFGRIFTSSNCLIAPNFAIGALLMMKFAALAAPYFETAEIIEFHHAQK
                     VDAPSGTAVLTAQRIAEASSDWAPDPTTSTVYEGARGGVGADGIHIHSLRIQGMVAHQ
                     EVVLGTTGQTLVIRHDSIDRTSFMPGVLMGTKAIADHP"
     gene            complement(4303..4998)
                     /locus_tag="PKY13_12025"
     CDS             complement(4303..4998)
                     /locus_tag="PKY13_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT80711.1"
                     /translation="MSDADPFVPPPAAEALARLDMPMGEAMRTQRAVRRLHLDPVDHD
                     VLLPLLELSLKAPTSSNSQDWCYLVVEDPEQKRRLAKLYRPLYNTFSPLERRAARNDP
                     DRLRGMKPGEWQAEHFHELPVFVIPCYTRNLKHRPVGRPQISVASFYGSVYPAVQNLL
                     LECRAVGLGASLQTLPIWHIPIARRILGLPRSMNPVCIIPIGWARGKYGPTTRRPIGE
                     VVHLDRYGNQPFR"
     gene            complement(5039..>5820)
                     /locus_tag="PKY13_12030"
     CDS             complement(5039..>5820)
                     /locus_tag="PKY13_12030"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT80712.1"
                     /translation="GPTGAPDVRVRLYRPAGSTEPVPALLWIHGGGFLIGTARQDERS
                     NIDMVRELGIAVASVDYRLAPEHPFPAPAEDCYAALRWLHAAAPAFGIRSDRIAVGGA
                     SAGGGLAAGVALMAHDRGEVPLAFQLLVYPMLDDRTVQRARGDESDFRLWNSTCNEFG
                     WRSYLGAMPGGPEVSPYAAPARRVDLGGLAPAWLGVGTLDLFHDEDVDYATRLREAGV
                     ACDLVEVDGAFHGFDGLAPRAPVVTAFRRSWLDALGGALNS"
BASE COUNT          923 a         1979 c         1984 g          834 t
ORIGIN      
        1 gggtcgtcgc cgatgtcggc ggttcgatcg ctgccgcgag agccatggat gagataatag
       61 gaccgaaagc gtctcattgt gttgtcagtg tgacggaagt cacgaactga cagccgggga
      121 gtgctccgca cagcgcccgt cgacgtgccc cgctgcccag cccatcactg ccgctggatg
      181 atcccggcca ggaactgcgt gcgaaccagc cgctcgacct gggtccggtc cgtgaggtcc
      241 cagcggccgg cggtcccgat gtggctcaac aacatccggg tcaggtactc ggcggccgtc
      301 tcgaggtcga gtccgtccac gagctgctcg cgcgagatca ggtccttgat gtagcccatg
      361 atcgcaagtt ggacgagctg gtcggactcg ctgagcgccg gcaggaggtc gtcgggctcc
      421 gacgccacga gccgctgcag caagtcgtac tcgtcgatac ggcgacgggc ctccatgagc
      481 cccacgacca ggcgatcctc gatggcagcc agggggcgga cgtggtcggc gagctctccc
      541 cagaaccgcg ccacctcgcg ggtgaccgtg tcgttgatga gctgggtccg gccgccgggg
      601 aagtgtcgat agatcgtggc ccgggacagg ttggcctcgg cggcgacgtc ctcgagcgcg
      661 aacgaatgga gcccgccgcg gtcgacgcac accatgacgg cgtccatgat gcgctcgcga
      721 ggcgtgcggt tgggcacggt ggcggtcagg tgcccgcggc gacggggacg ccgccggcat
      781 cgagatcgtc ggggtccgtg ggcacgtcgt ccttgcggtc cttacccccg ccacgggcca
      841 cgcggcggat cacgagcggg taccccccga tcgcgatgag cgtgaacaca ccccactgca
      901 ccgcgtacgc gaagtgtggg ccctccgaac gttcgggcaa cgggtacggc ttgatcacgc
      961 cgtcggtcgg cggcgcctgg ccaccgagct cgaggtacac cggctgcagg tcgaggtcgc
     1021 gcgccgcctt cacccgatcg atatcgagtc gggcgatccg gttcgacccg agatcgacct
     1081 tggaactctg gaagctgccc ggcaactcgg agacccggag ccaccctcgc acctggacgg
     1141 gccccgtcgg cggctccgcg ccctcgatcg gcgctgtgtt gtcctcgatg gcctggggga
     1201 tgaacccgcg cagcaccagg atcggcgcct cggtgtcgga ccaccgcagc ggtgtcacga
     1261 ccatccgacc gggcgcgccg tcgagcgtcc ggttcggcac ggtgaactcg gccgctacgt
     1321 cgtaggtgcc gtcgacgacc acagcccggt actcgacgcg gtcaccgtcg gcgatggtcg
     1381 acacccgctc gtcgcggagc acgtcggcca gctgcgtcgg ggcgccgtcg gcccgcgcag
     1441 cgatgcgggc gttgagggtc tgcttctcgt cgagccgccg cagctgccag aacatcagca
     1501 ggatcatcga cacgatcagc ccgaggatca gcacgtggga cacgatccac ttcggcttgt
     1561 acaggaaccg gtacatcgtg gcgaaacgct acttctccga ccgacgacaa ccgacatcgg
     1621 gatgaccccc cacccgcgcc cctctgtcgg tgccggattt tgccggcgct cccctaaggt
     1681 ggcgccctgt gaccgccgag gacgtgacgc tcgaaaccgc gccgcggcgc aaacccccgt
     1741 cgtgggtgtt gcccacggcc ctcgtcccga tgatcgccgt gctggtgtgc gggtgggtcg
     1801 cgggctcggt ctgggcgaac tggcaggtca atcacccgct cggactcatc gcactgagcc
     1861 ccatcaaccg attcctgctc ctcacgacca atcggctcga cttctggagc tacttctcgg
     1921 tcggactcgg acgccacctg ttccccgacc cgttcttcta cctgctcggc tactggtacg
     1981 gcggccgggc cctgaaatgg gtatcggagg gcaacgcgtt cgcgacccgg ctggtgggcg
     2041 gcgacggcca cggtctcgag aaccccgccc atcgcaaggt cctgtacccg ctcgcattcg
     2101 tcatgcccaa caactgggtg tccctcttct gcggcgccgc ccggattccg atcccggcgt
     2161 tcatcgccct caacatcagc ggcacacttg cccggctggt cctgtgccgc tggctcggcg
     2221 acctcttccg gagcgagatc aaggacatct ccgatttcat cgctcggttc caatggccga
     2281 tcaccgcggt gagcgtggtg atcgtcctcc tcggcatggc ggtgcagctc cgaccgaatg
     2341 gggccgtccg ccggcttgcg gcgctcgacg accagcccga gccctgagag cccgagcgcg
     2401 ctggaggttt gaaccggagc gcacgggagt cgtgcgctcc ggttcgaacc tcacagcgcc
     2461 gcgacctcgc cggccaggta gatgatcgac accacgatca gcaacgaccc tgcaacgagc
     2521 cgcatgagtt gttccgacgc cccgacggtg atgtgggcac cgactcgggc cccgggaacg
     2581 atcccgatgg ccaacgggag ggcgaatcgc cagttcacgt gaccgagcac gatgtgggtg
     2641 accaccgccg tggtcgacat catcgcgacg gcggcgagcg acgtcgcgat ggcgtgtttg
     2701 acgggcagcc ggaggcccag ggtgaggccc ggggtcagga tcagtccccc gccgatgccg
     2761 aggagcccgg cgaggaatcc accgccgacg cccagcgcca ccattgccgg cacaccccca
     2821 cgcggttgga tcgccacccc gccctcggtg cccgcgcctc cgtcgctcac gacgaggtca
     2881 tcggggtcgg cgcctgcctc gaccgcggcg tcagcgcgct cggcggcatt ggtgcgcagc
     2941 ccgtcgcgga ggagcgacac accgcaccac atcacgagga ccgcggtcag gatcatcagc
     3001 cagcgggcgt cgacgatgtc ggcgacccac ccgccgagga cggcgaacga catgccgctc
     3061 accccacacg tgagggcgac gggccagacg atgtagccct cacgatggaa tcggaggctc
     3121 cccgacagcg ccccgggcag gatggccggc acggtcgagc cgaccgcggc gatcggtgat
     3181 gcccccaggg cccggatcat cgggatggtg atcaccgctc cgcccacgcc gagcacggcc
     3241 gcgagcacgc cggacccgaa tccggcgacg agcaccgcca acgtcaccca catgagcggc
     3301 tgatctagag accgagcaac gtgtccagcc cgacggtgac gccggggtgg tcggcgatgg
     3361 ccttcgtgcc catcaacacc ccgggcatga acgaggtnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnncgg ggtggtcggc gatggccttc gtgcccatca acaccccggg
     3541 catgaacgag gtgcggtcga tcgagtcgtg tcggatgacg agcgtctgac cggtcgtgcc
     3601 caggacgacc tcctggtgcg caaccatgcc ctggatacga agcgagtgga tgtggatgcc
     3661 gtcggccccg accccgccgc gggcgccttc gtacaccgtc gaggtggtcg gatcgggggc
     3721 ccaatcactc gacgcctcgg cgatccgctg tgcggtcagc accgcggtgc ccgacggggc
     3781 gtcgaccttc tgggcgtggt ggaactcgat gatctcggcg gtctcgaagt acggcgctgc
     3841 gagcgcggcg aacttcatca tcagcaacgc accgatcgcg aagttggggg cgatcagaca
     3901 gttcgacgag gtgaagatcc ggccgaacag gtcgaggtcg tcgtcggaga aaccggtggt
     3961 gcccacgacg gcgtggatgc cgtgctgggc aaggaagatc aggttctgcc gaccggcgtc
     4021 ggcctcggtg aagtcgatgg ccacctgcac gttcgcctcg acgaacacat cggggttgcc
     4081 ggagatctga acgtcgcacc cgggcacgcc ggcgacacgg gacacctcga gaccggagtg
     4141 gtgcgggtcg accgcggcga ccagctcgag cgccggatcg tcgtggaccg cacggcacac
     4201 ggtcgacccc attcgcccgc cggccccgaa cacacccacc cggatcttgc gctgcgactc
     4261 gaccatcggc gcagtgtagg tcgggcccgg accaccaacc cgctaccgaa atggctggtt
     4321 gccgtagcgg tcgaggtgca cgacttcgcc gatgggccgc cgcgtcgtcg gtccgtactt
     4381 cccgcgggcc cacccgatgg ggatgatgca caccgggttc atcgaccggg gcaggcccag
     4441 gatccgccga gcgatgggga tgtgccagat cggcaacgtc tgcagtgatg ctcccagccc
     4501 cacggcgcgg cactccagga ggaggttctg caccgccggg tagaccgacc cgtagaacga
     4561 ggccaccgag atctgcggtc gcccgaccgg tcgatgcttc aggttgcggg tgtagcaggg
     4621 gatcacgaac acgggcagct cgtggaagtg ctcggcctgc cactcccccg gcttcatgcc
     4681 acggagacgg tccggatcat tgcgagccgc cctgcgttcg agcggcgaga acgtgttgta
     4741 gagcggacgg tacagcttgg ccaggcgtcg tttctgctcg ggatcttcga cgacgaggta
     4801 gcaccagtcc tggctgttgg aactcgtcgg tgccttgagc gacaactcga ggagcgggag
     4861 caacacgtcg tggtcgaccg ggtcgaggtg gaggcggcgg acggcgcgtt gtgtgcgcat
     4921 cgcctcgccc atcggcatat cgagccgggc aagcgcctcg gcggccggcg gtggcacgaa
     4981 cggatcggcg tcggacatcg ccggagcgta gggcccggcg gtgcgctcca ccaccggatc
     5041 aggagttgag cgccccgccc agagcgtcga gccagctccg ccggaacgcc gtcaccaccg
     5101 gcgcccgggg tgccagcccg tcgaatccat ggaacgcacc gtcgacctcg accaggtcgc
     5161 aagcgacccc cgcctcgcga agccgggtgg catagtcgac gtcctcgtcg tggaagaggt
     5221 cgagcgtgcc gacgccgagc cacgcgggtg cgagcccgcc gaggtcgacg cggcgggcag
     5281 gcgcggcgta tggcgatacc tcggggccgc cgggcatcgc cccgaggtag gaccgccagc
     5341 cgaactcgtt gcatgtcgag ttccacaggc ggaagtcgga ttcgtcgcca cgagcgcgct
     5401 gcacggtgcg atcgtcgagc atcggataca cgagcagctg gaacgcgagg gggacctcgc
     5461 ctcggtcgtg ggccatcagc gcaacgccgg cagcgagtcc gccaccggcg ctcgccccgc
     5521 ccacggcgat ccggtccgac cggatcccga acgccggcgc cgcagcgtgc agccagcgca
     5581 gcgcggcgta gcagtcctcg gccggcgccg ggaaggggtg ctccggcgcg aggcgatagt
     5641 cgacggacgc gacggcgatg ccgagctcgc ggaccatgtc gatgttgctg cgctcgtcct
     5701 ggcgggccgt gccgatcagg aaaccgccgc cgtggatcca caggagcgcc ggcaccggct
     5761 cggtgctgcc ggcgggccgg tagaggcgca cccggacatc gggggctccg gtcggaccgg
//
LOCUS       DAOSOA010000208         5815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13912_length_5815_cov_5.739236, whole genome shotgun sequence.
ACCESSION   DAOSOA010000208 DAOSOA010000000
VERSION     DAOSOA010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13912_length_5815_cov_5.739236"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..967)
                     /locus_tag="PKY13_12035"
     CDS             complement(131..967)
                     /locus_tag="PKY13_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT80713.1"
                     /translation="MKAIEMPDGRPFGNVRLSKLGWQEIEHLYAGMRTSGRGNDWIRR
                     CATVLARALELARKRGLLDSNPSKDATRPKTTRTKPYSPTLDDVRHLLLDVAEVDPEL
                     ADAAMILASTGMRRGELLGLQWKDVDLTTGEIHVAAAITDGGPGVGLVRKATKQSDWR
                     DVPLTTRAVVAFEAQRNRVGTRLGRPTKVTDFVFPSAGSAHLPLRPESLTGRWAASRG
                     TSPITLLHLRHFAATSMLDAGESYRTVADILGNSESTLRLHYDGRTDAGKRKAIAALE
                     LG"
     gene            complement(1419..1494)
                     /locus_tag="PKY13_12040"
     tRNA            complement(1419..1494)
                     /locus_tag="PKY13_12040"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1459..1461),aa:Gly,seq:gcc)
     gene            complement(1556..5449)
                     /locus_tag="PKY13_12045"
     CDS             complement(1556..5449)
                     /locus_tag="PKY13_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT80714.1"
                     /translation="MLLSIVAVALGVGYLAGSLSLLQRVGAGLAAQAGAGTERADLVI
                     EGTIADDGPLQQVRRLVPDTIVDQVRGIEGIAAVEPRLESKSTLILGPTGSPVVRLGL
                     TERPLGANFPTEPSLNPYRFVGEGRAPATDTEVAIDAETAKVAKVKVGEPVLIAGKVG
                     LKSFTVTGIVELTKGTFPAGSSLALFATEVARPLFELGPDDNAIAIKLEPGADRDKVI
                     EQLRAIIFSGAEVSTGAEYAEHRQASFEKSFTLIRALLVGFAALALVVGAFTVANSMA
                     LLFDHRRRGFAMLRLMGASPSQLVGAATAEALLGGAIAGVVGLGLGLGVGAAIEWLIQ
                     STGTPLPVAGPVITWWIPVLAVVIGAAVTVSTALSPARHASRTPPVHAVTGADDRPSD
                     RSPLVTGLRWLATVVVVAVAAALLGSAFGGTGVIPLAAGAGAAVAIVLVILPRLLSGI
                     VGVATSLLLGKSVALRRMSTLRSRQARTRAASTTAALLLAAAVVTSLTVLSSSFLRSV
                     EGQVSSAITADFVVDSATFATGGLSADLVPLLRQQPGVAAVSGWRVGNASIGLTKWRA
                     AGIDGASMFELLDLGVEGTPLASAGINDVVVSTQVAERENLQVGQTVPVRFENNAVIP
                     MRVRAIFDSNVRVLLGDVIVDSTVMAANLPQSVDVMAFVKLTKDAPVAATRQAIEVTA
                     KKYGAANVLEPSELVSKRADLLRGFGRVIQWMLAFSIALALIGVANTLQLGVNERRRE
                     LGLIRAVGATRQQVLRLVMAEAGALSVVGTLFGMVIGAGAAYATVSGLSVYGLDQFVA
                     PVGTLLFIAVVAIGLGLLGAILPAWRAAGVPLLDAISDSSDQDHSRSRRGIIASSAPS
                     RAAATRPVAGPRPTAAHHAGTPPPYPAPPLAPDTVVATPVPADPWIDHGSVPGAPAIV
                     GPPSADGAGAASPPPNHEVLMALRCYNCGNEPGDGESCIACGAPQTVTPVGMFTLRPE
                     ATNGTASNGSEPVSSLGADIVDAAVVENGVVIEPAPTTTSAGADSAPAPPPYVATAPA
                     DQEAESRHREPPADSYAVTPGDDTPIRSSLGSIFDGRPTPSEAHDPMRSPFGQHASAE
                     PEPPVLEEPLPTAAPAARHGIFSAGTADPSASDGETVAVPIARPAAAGPPPAFASAIP
                     AGAPAPTAAAPASSAFGGPTQADTHGLAASVARLSLGSQQNGLVPFSIAGALLGPQEH
                     VVAAVAGTSLGMPTVIVVTESRALIVSDRRYVPEVEIFELGAGLTIHGRHANEQASLT
                     FTDSERLITVDQIVDVGLAVELANTARNRTVGGDF"
BASE COUNT          913 a         1927 c         2144 g          831 t
ORIGIN      
        1 atgggcattc tgagggccgc cggcattggc tgacacccgc tgcacgcacc ttccgtcgga
       61 gttcgagtca tctgaccgac tcgtaaccac gcggctgcga gtcctcgcag atcgacgaac
      121 gatgcccgtc tcaaccgagt tccagcgccg cgatcgcctt acgtttcccg gcgtcggttc
      181 gtccgtcgta atgcaggcgc agggtcgact cactgttgcc gagtatgtcc gcgacggtgc
      241 ggtaggactc gccggcatcg agcatcgagg ttgcggcgaa atggcgcaga tgcaggagcg
      301 tgataggcga ggtccctcga ctggcagccc accgcccggt caacgactcg ggccgcaggg
      361 gaagatgcgc cgagccggcc gacgggaata cgaagtcggt caccttcgtc ggcctaccga
      421 gccgtgtgcc cacacggttt cgctgcgcct cgaacgcaac caccgctcga gtcgtcagcg
      481 gcacatcgcg ccagtcggac tgcttcgttg ccttgcgcac caggcccacg ccgggaccgc
      541 catcggtgat ggcagctgcc acatggatct cgccggtcgt tagatcgacg tccttccact
      601 gcaagccaag gagctcacct cgcctcatcc cagtgctggc gaggatcatc gctgcgtcgg
      661 ccagctccgg atcgacctca gcaacatcca acagcagatg gcggacgtcg tcgagagtag
      721 gggagtacgg cttcgtcctg gtcgtcttcg ggcgggtcgc gtccttcgaa gggttggaat
      781 cgagcagacc ccgcttgcgg gcaagctcga gggctctcgc gagaacggtc gcgcatcgtc
      841 ggatccagtc gttccctctg cctgaggtgc gcatgccagc gtagaggtgc tcgatctcct
      901 gccaccccag cttcgagagg cggacgttgc cgaacgggcg tccgtccggc atctcgatcg
      961 ccttcatcgt tctgatcgcc gaacgagacg tcaggatcgt gctgggcgcg agttcgatca
     1021 ggccctgatc gatcgactcc aagtattggt cgagcgcggc gccgaccgaa tgagcgcggg
     1081 tgcccccgga cggaagccga cgctcgtggt ccgcgacttt gaggcgggcc aacgcaacct
     1141 cggcatctct tgccgttcct tcgaccgtgc gtgagacccg gcgtcgggcg ccggtgaccg
     1201 ggtcccgccc gatctcgacg tcgatacgcc agacgccctc gcggacgaac gtcacgcctc
     1261 cgctgcctcg ttttctccgt cgggtaccgc tcggcgccac ccgccgtgcg gccgttgaag
     1321 cgccgctcat gaacgggata gtagagcgag ttagtagagc gcggaagggc aacttgtgcc
     1381 gtgaaccaac gaaacccccg acgaatcggg ggtttctgtg gagcggacga ccgtattcga
     1441 aacggcgacc ctcaccttgg caaggtgatg ctctaccagc tgagctacgt ccgcagggca
     1501 tcgagagtgt agcaacaaac gcgtcggcca cgcgccattc acgcaccgac ttcggtcaga
     1561 aatctccgcc gacggtccga ttgcgggccg tgttggccag ttcgaccgcc aagcccacgt
     1621 cgacgatctg gtcgacggtg atgaggcgct cgctgtcggt gaaggtcagc gacgcctgct
     1681 cgttggcgtg ccggccgtga atggtgagcc cggcacccag ctcgaagatc tcgacctcgg
     1741 ggacgtatcg gcggtccgac acgatcagcg cgcggctctc ggtgaccacg atgacggtgg
     1801 gcatgccgag tgacgtgccc gccaccgcgg cgaccacgtg ttcctgcggc ccgagcagcg
     1861 cgccggcgat gctgaacggg acgagcccgt tctgttgcga accgaggctg agccgggcga
     1921 ccgacgccgc caacccgtga gtgtcggcct gggtcggacc gccgaacgcg gacgacgccg
     1981 gtgccgcagc ggtcggtgcc ggcgccccgg cgggaatggc gctcgcgaac gccggcggcg
     2041 gaccggcagc agcgggacgg gcgatcggca cggcgacggt ctcgccgtcg gatgccgacg
     2101 ggtcggcggt gccggcgctg aagatgccgt gacgggcggc gggtgccgcg gtcgggagcg
     2161 gctcctccaa tacgggtggc tcgggttcag cgctcgcgtg ctgcccgaac ggcgacctca
     2221 tcgggtcgtg cgcctccgac ggcgtcgggc ggccgtcgaa gatcgagccg agcgacgagc
     2281 ggatcggtgt gtcgtccccc ggcgtcaccg cgtaggaatc ggcgggtggt tcccgatggc
     2341 gggactcggc ctcctggtcg gcgggggctg tcgcgacgta gggcggcggg gccggtgccg
     2401 aatccgctcc ggccgatgtt gtggtcggag cgggttcgat gacgacgccg ttctcgacga
     2461 ccgcagcgtc cacgatgtcg gcgccgagcg acgacaccgg ttcggaaccg ttgctcgccg
     2521 tgccgttggt ggcctcgggg cgcaacgtga acatacccac gggcgtaacg gtctgtgggg
     2581 cgccgcaggc gatgcaggac tcgccgtcgc ccggctcgtt gccgcagttg taacaccgca
     2641 atgccatcag gacctcgtgg ttcggcgggg gggaggcggc gccggcaccg tcggcgctcg
     2701 gcgggccgac gatagcgggt gcgcccggga cacttccatg gtcgatccac gggtccgcgg
     2761 ggaccggggt ggcgacgaca gtgtcgggtg cgagcggtgg cgcagggtag ggcggagggg
     2821 ttccggcgtg gtgcgctgcc gttggtcgcg gtccggcgac cgggcgggtt gcggcggcgc
     2881 gtgacggcgc cgatgacgcg atgatccccc gtcgcgaccg cgagtggtcc tggtcgctgc
     2941 tgtcggagat ggcgtcgagc aacggaacgc cggcggcgcg ccaggcgggg aggatcgcgc
     3001 cgagcaggcc cagtccgatt gcgacgacgg cgatgaagag caacgtgccg accggggcca
     3061 cgaactggtc gaggccgtac acagacagcc ccgacaccgt cgcgtaggcg gctccggcgc
     3121 cgatgaccat cccgaagagc gtcccgacga ccgagagtgc gcccgcctcc gccatcacga
     3181 gacgcagcac ctgctgccgg gtcgcgccga ctgcccggat gagcccgagc tcgcgacggc
     3241 gctcgttgac cccgagttgc agcgtgttgg cgacaccgat cagggccaat gcgatcgaga
     3301 acgcgagcat ccactggatc acacgaccga agccccgcag gaggtcggcg cgcttggaca
     3361 ccagctcgct cggttcgagc acgttcgcgg cgccgtactt cttggccgtc acctcgatcg
     3421 cctggcgggt ggcagcgacc ggtgcgtcct tcgtgagctt gacgaacgcc atgacgtcga
     3481 cgctctgggg caggttggcg gccatcacgg tggaatcgac gatcacgtcg ccgagcagga
     3541 cgcgcacgtt cgagtcgaag atggcgcgaa cccgcatcgg gatcaccgcg ttgttctcga
     3601 agcgcacggg aacggtctga ccgacctgaa ggttctcccg ctcggccacc tgggtgctca
     3661 cgacgacgtc gttgatgccc gccgacgcaa ggggagtgcc ctcgaccccg agatcgagga
     3721 gttcgaacat cgacgcaccg tcgatgccgg cggcacgcca cttcgtcagc ccgatcgagg
     3781 cgttcccgac ccgccagccg ctgacggctg ctactcccgg ctgctggcgg aggagcggca
     3841 cgaggtccgc ggacaagccg ccggtcgcga acgtggccga gtcgaccacg aagtcggcgg
     3901 tgatcgctga cgagacctga ccctcgaccg accggaggaa gctgctcgac aggacggtca
     3961 acgacgtgac caccgcagcg gcgaggagca acgccgctgt ggtggaggcg gctcgggtgc
     4021 gggcctggcg cgaccgcagt gtgctcatgc gccgcagggc caccgacttg cccaacagca
     4081 gcgacgtggc gacgcccacg atgccgctca ggagccgcgg caggatcacg aggacgatgg
     4141 ccacagcggc tcctgcgccg gcggcgaggg ggatcacgcc ggttccgccg aacgcgctcc
     4201 cgaggagcgc tgctgcaact gcgaccacga caaccgtcgc cagccagcgg agcccggtca
     4261 cgaggggtga gcgatcgctc ggccggtcgt cggcgccggt gacggcgtgg accggcggcg
     4321 tgcgcgacgc gtggcgggcc gggctgagcg ccgtggacac ggtgacagcg gcgccgatca
     4381 cgacggccaa caccgggatc caccacgtga tgacgggccc ggcgacgggc aggggtgtgc
     4441 ccgtcgactg gatcaaccac tcgattgccg cgccaacgcc gaggcccagg ccgagaccga
     4501 ccacaccggc gatggcgccg ccgagcagcg cctccgccgt ggcggcgccg acgagctggg
     4561 acggcgacgc ccccatgagc cgcagcatcg cgaagccacg acggcgatgg tcgaagagca
     4621 gggccatcga gttggccacg gtgaacgcac cgaccacgag cgccagggcc gcgaagccga
     4681 cgaggagggc tcggatcagg gtgaagctct tctcgaacga tgcctggcgg tgttcggcgt
     4741 actcagcacc ggtcgacacc tcggcgccgc tgaagatgat ggcacggagt tgctcgatca
     4801 ccttgtcccg gtccgccccg ggttcgagct tgatggcgat ggcgttgtcg tcggggccga
     4861 gctcgaagag tgggcgggcg acctcggtgg cgaagagcgc cagggacgag cccgccggaa
     4921 aagtgccctt ggtcagctcg acgatgcccg tcacggtgaa cgacttcagg ccgaccttgc
     4981 cggcgatcag gaccggctca ccgaccttca ccttggcgac cttcgccgtc tcggcgtcga
     5041 tcgcgacctc ggtgtcggtt gcgggtgcgc gcccctcgcc gacgaatcgg tagggattga
     5101 gggacggctc ggtcgggaag ttggcgccga gcggccgttc ggtgagcccc aacctcacca
     5161 ccggcgagcc cgtcggtccg aggatgaggg tcgacttgct ctcgaggcga ggctcgaccg
     5221 cagcgatgcc ctcgatgcct cggacctgat cgacgatcgt gtcagggacc agccggcgga
     5281 cttgttggag cggaccgtcg tcggcgatgg tgccctcgat gacgaggtcg gcgcgttcgg
     5341 tgccggcgcc ggcttgcgcg gcgagtccgg cgccgacccg ctggagcagg gagagtgagc
     5401 cggccaggta gccgacaccc aacgcgacgg cgacgatcga caagagcatg cgtcgccgct
     5461 ggcgcagcag cgtccgcaac gcgacccgga tgccccggag ccctccggtc tcgccgcccg
     5521 tctgctgctt gggtggtgcg aaccggaaca gccggccgtc ggcgtccagc aggtcggcaa
     5581 ccggcgccgc cggggcgacg ggctcgtacg ccgggggtgg cggcggtgca ggcggagcag
     5641 gcggggcgga cggcgcaggg ggagccggcg gggcggacgg cgcaggggga gccggcgcag
     5701 ggggagccgg cgcaggggga gccggcgcgg tctgcggagg gaggaacggc ggagcgggag
     5761 gtgccggcgg gggatctgag accggtggcg ccacgggtgg cggaggcggt gccgc
//
LOCUS       DAOSOA010000209         5792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_13989_length_5792_cov_6.279763, whole genome shotgun sequence.
ACCESSION   DAOSOA010000209 DAOSOA010000000
VERSION     DAOSOA010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13989_length_5792_cov_6.279763"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PKY13_12050"
     CDS             complement(<1..769)
                     /locus_tag="PKY13_12050"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT80715.1"
                     /translation="MPNPYARAEAAAAVIRSRLVPEPKVAVVLGSGWVGVAEGLGSTL
                     AELPLAELPHVPAPTVAGHSGVARAVDVGGVPTLVLGGRSHLYEGHPVDTVVHTVRAA
                     VLAGCSTVVLTNACGSLDAAMGVGQPVLLSDQLNLTGANPMAGGDPPEGYPGRFCDLT
                     EIYSRRLRDAARELDPSLPEGVYAGMLGGSYETPAEIRMLRTLGADLVGMSTVLESIA
                     ARHLGAEVAGVSLVTNLAAGMGAELSHAEVLDAGRASA"
     gene            833..2044
                     /locus_tag="PKY13_12055"
     CDS             833..2044
                     /locus_tag="PKY13_12055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT80716.1"
                     /translation="MGIEVRATAPEERRAAANATRVALLDNPPDDETWAKHEARWQSL
                     RSFSAWDGDRCVGHVGSFDTETTAPGGAHLTTIGVTSAGVLPTHVRQGIFRRLIDALI
                     ADAIADGVALASLRASEATIYGNFGFGMAGDALTVTIDVARARPLRGATTDGTFDLVP
                     GAEILDTVRPIYDRADRRPGRIARPDWLWGRFFENAVTGHSVENVVVHRSATGEPDGY
                     AHYQLNWSDDIFGADHGTGKVHEVWATSPKVELALWQFLFDIPLVRQLSVDELAADSV
                     LFHAAADFRAVRVGMRWDEQWLRILDVDAALAGRSWADAAPIVVGLVDSGADGASRRW
                     RIGAGAATATDDLADLTTDIAGLSAAYLGSTSWADLTSAGRAEANDDATVRRADAVFA
                     HRPLTFSGSFF"
     gene            2044..3252
                     /locus_tag="PKY13_12060"
     CDS             2044..3252
                     /locus_tag="PKY13_12060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT80717.1"
                     /translation="MTAYVTRPTTPDEWRVCGDAMRTALLTGAATDEDWAKTEPSWAD
                     QDGIAAWELDACIGHVAAYRFEMLVPGGAWVPTAGVTRVGVLPTHRRRGVLTALLGEL
                     ADDARERGQVLAALRASEAGIYGRYGYGIASHRLTVRIDTARARRTATATPGAFRLLA
                     PTELLDVVPPLYERCTHRPGAVGRAEWMWRRYLEEAIEGHTADRVVVHTSPDGIDDGY
                     AQYSVAWKDRDGLMETGAGDVAEVRAATPEVEAALWDYLLGLDLVQTLDLMELPTDDL
                     LFQLAADPRAVGLRARYDEIWLRILDVDAALTARTVNDAAPVTIAVTDGRYDDNTGSW
                     TIGPDGAKRTDTTAGIATDIAGLSAAYLGSTSWWELVAGGRASGDADAVRRADALFAH
                     RPLAFCGSFF"
     gene            3265..3999
                     /locus_tag="PKY13_12065"
     CDS             3265..3999
                     /locus_tag="PKY13_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT80718.1"
                     /translation="MTETWVGFDADDTLWHNESYFMESYDLFADVAAPYLADFDDPAQ
                     AAHDLLIATETVNIPAFGYGIKGATISMIEAAITASGGAIPASELMRLINRAKAMMAH
                     PVEFLDGAVEALDALSHHRLVLLTKGDLKDQHRKIDASALAERFDAIEILHEKDETSY
                     LAAFARHGIDPSRFVMVGNSLKSDVLPILGLGGWAIHVPYVTTWAIEHAEEPTGAPRF
                     VGPATLWDVPALVETCLGDAIENGDH"
     gene            3999..4655
                     /locus_tag="PKY13_12070"
     CDS             3999..4655
                     /locus_tag="PKY13_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT80719.1"
                     /translation="MALEQTRDGDVVIVSWNDGENRYRGDSVAEWHEVLDEVEATDGP
                     LALVVTGTGKFFSNGLDLDWMSAHPDESGDMLRGVHRLLGRMLVMNLYTVGAINGHAF
                     AGGAMLTCGFDERIMREDRGYWCLPEVDLGLPLTPGMYATVAARLPQATLHDSIVTGR
                     RYGGAEALVAGIVEHIAAEADVLPLAVERAQAMAAKNRSIIANHKRQMYGAAAELCGA
                     "
     gene            complement(4734..5126)
                     /locus_tag="PKY13_12075"
     CDS             complement(4734..5126)
                     /locus_tag="PKY13_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT80720.1"
                     /translation="MAVYLDTSAATKLVVSEAGSTSLRRWIAALDEPVVSSDLLRTEL
                     LRAVRRRKPSAMTQARAVLDSITLTDMPTSLFERAALLEPIGLRSLDALHIAAALDLG
                     DELSAIVTYDDRMVEAIEALGLIALSPR"
     gene            complement(5126..5401)
                     /locus_tag="PKY13_12080"
     CDS             complement(5126..5401)
                     /locus_tag="PKY13_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOT80721.1"
                     /translation="MSQVGIRELKQNASAVVAEAAAGELVTITDRGRPVAQLVPIGGS
                     RLDALIRAGRARAPRRSIADLPPPIRDRSGGRSLTDEVIAARDDERY"
     gene            complement(5542..>5792)
                     /gene="upp"
                     /locus_tag="PKY13_12085"
     CDS             complement(5542..>5792)
                     /gene="upp"
                     /locus_tag="PKY13_12085"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOT80722.1"
                     /translation="LVVDPMLATGGSVIHALDVLKRNGVTRLRLLSIFGVPEGVANVH
                     AAHPDVPIFLAVLDDQLNDKGYILPGVGDAGDRLYGTR"
BASE COUNT          912 a         2119 c         1944 g          817 t
ORIGIN      
        1 cggccgaggc ccgcccggcg tcgagcacct cggcgtggct caactccgcg cccataccgg
       61 ccgccagatt cgtcaccagc gacacgccgg cgacctcggc accgagatgt cgcgctgcga
      121 tcgactccag cacggtcgac atgcccacca gatcggcacc caacgtccgc aacatccgaa
      181 tctccgccgg ggtctcgtag ctgccgccga gcatgccggc gtagacgcct tcgggcagcg
      241 acggatcgag ctcgcgggca gcgtcgcgga ggcgccggct gtagatctcg gtgaggtcgc
      301 agaaccgccc ggggtagccc tcgggcgggt cgccaccggc catcgggttc gcaccggtga
      361 ggttgagctg gtcgctcagg aggaccggct ggccgactcc catcgcggcg tcgagcgacc
      421 cgcacgcgtt ggtgagcacc acggtcgagc acccggcaag gaccgccgcc cgcaccgtgt
      481 gcaccacggt gtcgacgggg tgtccctcgt agaggtgcga ccgaccgccg agcaccaacg
      541 tcgggacacc gccgacatcg acggcgcggg cgacaccgga gtgaccggcc accgtcggtg
      601 ccggcacgtg tggcagctcg gcgagcggca gctcggcgag tgtcgacccg aggccttcgg
      661 cgacgcccac ccaaccggat ccgagcacga ccgcgacctt cggctcggga acgagacggg
      721 aacgaatgac ggcagcagcg gcttcggcgc gggcgtaggg attcggcatg ccgccagtct
      781 cgcgcccgaa atccgcagcg tgctccccgc gcgaactgcc acgatctccc ccatgggtat
      841 cgaggtccgg gcaacggcgc ccgaggaacg acgggccgcc gccaacgcca cgagggtggc
      901 cctcctggac aacccgcccg acgacgagac gtgggccaag cacgaggccc ggtggcagtc
      961 gttgcgttcg ttctcggcgt gggacggtga tcggtgcgtc ggccacgtcg gttcgttcga
     1021 caccgagacc acggcgcccg gtggcgccca cctcacaacg atcggcgtga ccagcgccgg
     1081 agtgctcccc acccatgtcc gccaggggat cttccgtcga ctgatcgacg ccctcatcgc
     1141 cgacgccatc gccgacggcg tcgcgctggc gtcactgcgg gccagcgagg cgacgatcta
     1201 cggcaacttc ggattcggca tggcgggcga cgcactcacg gtgaccatcg acgtcgcccg
     1261 cgcccgtccg cttcgaggag caacgaccga cggcaccttc gatctggtcc ccggcgccga
     1321 gatcctcgac accgtccgac cgatctatga ccgcgccgat cgtcgtccgg ggcgcatcgc
     1381 ccgcccggac tggctgtggg gccggttctt cgagaacgca gtcaccggcc acagcgtgga
     1441 gaacgtcgtc gtgcaccggt ccgccacggg cgagccggac ggctatgccc actatcaact
     1501 caactggtcc gacgacatct tcggcgccga ccacggcacc gggaaggtgc acgaggtgtg
     1561 ggcgacctcg ccgaaggtcg agctggcact gtggcagttc ctgttcgaca tcccgctcgt
     1621 ccgtcagctg agcgtcgatg agctcgccgc cgacagtgtc ctcttccacg ccgccgccga
     1681 cttccgcgcc gtacgcgtcg gtatgcgctg ggacgagcag tggttgcgga tcctcgacgt
     1741 cgacgcggcg ctggcggggc gatcgtgggc cgacgccgcg ccgatcgtgg tcggcctcgt
     1801 cgattccggg gccgacggtg cgagccgacg ctggcgcatc ggggccggag cggcgaccgc
     1861 gaccgacgac ctcgccgacc tcacgaccga catcgccggc ctcagcgccg cctatctcgg
     1921 ctccaccagc tgggcggacc tcacctctgc gggacgtgcc gaggcgaacg acgacgccac
     1981 cgtccgacgc gccgatgccg tgttcgctca ccggccactc accttcagcg ggagcttctt
     2041 ctgatgaccg cctacgtgac ccgccccacg acgccggacg aatggcgggt gtgcggcgat
     2101 gccatgcgga ctgccctgct cacgggcgcc gccaccgacg aggactgggc gaagaccgaa
     2161 cccagctggg ccgaccagga cggcatcgcc gcctgggaac tcgacgcgtg catcggccac
     2221 gtcgcggcgt accgcttcga gatgctcgtc cccggcggcg cgtgggttcc gaccgcgggc
     2281 gtcacccgcg tcggggtact tcccacccac cggcgtcgag gtgtgctcac cgcgctcctc
     2341 ggcgagctcg ccgacgatgc ccgggaacgg ggccaggtcc tggcagcgct ccgcgccagc
     2401 gaggccggca tctacgggcg ctacggctac ggcatcgcga gccaccgcct caccgtgcgc
     2461 atcgacaccg cccgggcgcg ccgcaccgcg accgcaaccc ccggcgcctt ccggctgctc
     2521 gcgccgacgg agctgctcga cgtcgtgccg ccgctgtatg agcggtgcac ccaccgcccc
     2581 ggtgcggtcg ggagagcgga gtggatgtgg cgccgctacc tcgaggaggc catcgaggga
     2641 cacaccgccg accgggtcgt cgtgcacacc tcgcccgacg ggatcgacga cggctacgcg
     2701 cagtactcgg tcgcctggaa ggatcgcgac gggttgatgg agaccggggc cggcgacgtg
     2761 gccgaggttc gcgccgccac ccccgaggtc gaggcggcgc tgtgggacta cctgctgggt
     2821 ctcgatctcg tgcagacgct cgacctgatg gagctcccga ccgacgacct gttgttccag
     2881 ctcgccgccg acccccgcgc cgtcggcctg cgggcacgct acgacgagat ctggctgagg
     2941 atcctcgacg tcgacgccgc cctcacggcg cgaaccgtca acgacgccgc ccctgtgacc
     3001 atcgccgtca ccgacggtcg ctacgacgac aacaccggtt cgtggacgat cgggcccgac
     3061 ggagcgaagc ggaccgacac caccgccggc atcgcgaccg acatcgccgg tttgagtgcc
     3121 gcgtacctcg gctcgaccag ttggtgggag ctcgtcgcgg gcggtcgcgc ctcgggtgac
     3181 gccgacgccg tgcgccgcgc cgacgccctg ttcgcccacc ggccgctggc attctgcggc
     3241 agcttcttct gacacgatgt gcggatgacc gagacgtggg tggggttcga cgccgacgac
     3301 accctgtggc acaacgagtc ctacttcatg gagagctacg acctcttcgc cgatgtcgcc
     3361 gcgccgtatc tcgccgactt cgacgacccg gcgcaggcgg cgcacgacct cctgatcgcc
     3421 accgagaccg tcaacatccc ggccttcggc tacggcatca agggcgcgac gatctcgatg
     3481 atcgaggcgg ccatcaccgc ctcgggcggc gccatcccgg caagcgagct catgcggctg
     3541 atcaaccggg cgaaggcgat gatggcgcat ccggtcgagt tcctcgacgg ggcggtcgaa
     3601 gccctcgacg ccctctcgca ccaccgtctc gtgctgctca ccaagggtga cctgaaggat
     3661 cagcaccgca agatcgacgc gtccgctctc gccgagcggt tcgacgcaat cgagatcctc
     3721 cacgagaagg acgagacctc ctacctcgcc gcgttcgctc gccacggcat cgatccgtcg
     3781 aggttcgtga tggtcggcaa ctcactcaag tcggacgtcc tgccgatcct cggcctcggg
     3841 ggctgggcga tccacgttcc ctacgtgacc acctgggcga tcgagcacgc cgaagaaccc
     3901 accggtgcac cccgcttcgt cggtccggcg acgctgtggg acgtgcccgc cctcgtcgag
     3961 acctgcctcg gcgacgccat cgagaacgga gaccactgat ggccctcgag cagacacgcg
     4021 acggcgacgt cgtgatcgtc tcctggaacg acggcgagaa ccgctaccgc ggcgattccg
     4081 tcgccgagtg gcacgaggtg ctcgacgagg tcgaggccac cgatggtccg ctcgccctgg
     4141 tcgtgaccgg cacgggcaag ttcttctcga acggtctcga cctcgactgg atgagcgccc
     4201 accccgatga gagcggggac atgctgcggg gagtccaccg gctcctcgga cggatgctcg
     4261 tcatgaacct ctacaccgtc ggggcgatca acggtcacgc cttcgccggc ggagcgatgc
     4321 tcacgtgcgg gttcgacgag cggatcatgc gcgaggaccg tggctactgg tgcctgcccg
     4381 aggtcgacct cgggctcccc ctcaccccgg gcatgtacgc caccgtcgcc gcccgactcc
     4441 cccaggccac cctccacgac tcgatcgtga ccggccgccg ctacggcgga gcggaggcgc
     4501 tcgtcgccgg catcgtcgag cacatcgcgg ccgaagccga cgtgctgccg ctcgccgtcg
     4561 aacgggccca ggccatggcc gccaagaacc gatcgatcat cgccaaccac aagcggcaga
     4621 tgtacggcgc tgccgctgag ctctgcggcg cctgaccatt cgacgtcacg aaccccgcac
     4681 cgagcgggtt tcacgtcggg agaaccgacg gcgcggcgct ctggctcccg cggtcagcgc
     4741 ggcgagagcg cgatcaaccc gagggcctcg atcgcctcca ccatccggtc gtcgtaggtg
     4801 acgatcgccg agagctcatc gccgaggtcg agcgccgcgg caatgtgcaa cgcgtcgagg
     4861 ctccgcagac cgatcggctc cagcagcgct gctcgttcga agagcgacgt cggcatgtcg
     4921 gtgagggtga tcgagtcgag cacggctcgt gcctgcgtca tcgctgacgg cttgcgccgc
     4981 cgcacggcgc gcagcagctc ggtccgaagc aggtcgctcg agacgacagg ctcgtcgagc
     5041 gctgcaatcc accggcggag cgatgtcgag cccgcctcgg agaccaccaa cttcgtcgcc
     5101 gcggacgtat cgaggtagac cgccactagt agcgctcgtc gtctcgagca gcgatgacct
     5161 cgtctgtcag gcttctcccc ccagaccgat cccggatcgg cggcggcagg tcggcaatcg
     5221 atcgcctcgg cgcacgggct cgacccgccc ggatcagggc gtcgaggcgg gacccaccga
     5281 tcggaaccaa ttgcgccacc ggccggccgc gatcggtgat cgtcacgagt tcgcccgctg
     5341 ctgcctcggc cacgaccgcc gatgcgttct gcttcagttc gcgtatcccg acctggctca
     5401 tgtgctacat cgtagcacat gagccaggtc gggatagccg acgggtcgcg ccgcggcctg
     5461 gtgcgcctga cgccgcggcg acgtgaaacc cgcaccgagc gggttccacg tcgggagaac
     5521 acgaccggcg gggtgggcgg attaccgcgt gccgtagaga cggtcgccgg cgtcaccgac
     5581 accggggagg atgtacccct tgtcgttgag ctgatcgtcg agcaccgcaa ggaagatcgg
     5641 cacgtcgggg tgggcggcgt ggacgttggc cacgccctcg ggcacaccga agatcgacag
     5701 caggcgcagg cgggtgacgc cgttgcgctt caacacatcg agcgcgtgga tcaccgaccc
     5761 gcccgtcgcc agcattgggt cgaccaccag ca
//
LOCUS       DAOSOA010000210         5762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14093_length_5762_cov_5.514456, whole genome shotgun sequence.
ACCESSION   DAOSOA010000210 DAOSOA010000000
VERSION     DAOSOA010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14093_length_5762_cov_5.514456"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKY13_12090"
     CDS             <1..699
                     /locus_tag="PKY13_12090"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOT80723.1"
                     /translation="GLDLMIGIGGDGTMLDAVRLACRSEAPVLGINVGQLGYLTSIEP
                     KFEPAAGRTALKRFLAGGYIIEERMRIGAHIERSDGTVEATDAGLNEILLERAELGHT
                     IRIDVSLDGEPFTPYVADGVMIATPTGSTAYAFSARGPIVDPRHRAQVLVPVSPHMLF
                     DRALVLAPDAVVRMTVAGGRPAHLSVDGHSGGTLDVGDAVVCTADPVPARLVRFGPPS
                     FQRVLKTKFGLPDR"
     gene            768..2372
                     /gene="recN"
                     /locus_tag="PKY13_12095"
     CDS             768..2372
                     /gene="recN"
                     /locus_tag="PKY13_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT80724.1"
                     /translation="MLLELAVRDLGVIADLRLDFGAGMCALTGETGAGKTMIIEALAL
                     LLGGKTDPTRVRPGAEASVVEGRFAVPGEAGADDDEIVLRRVVPAEGRSRCYVNGEMA
                     TAAGLAEIGGRLVEICGQHSHQRLQSTRAQRDALDRFGSINLAALVVAQSRRRELEAQ
                     LAALGGDERARAREIDLLRFQVDELDAAAIDDPSEDERLAAEEDLLADALAHLEAGAS
                     ALAGLHDDGAATDLVAAALAAVEHRAPFTATAARLHGLLAELTDVVDELRTVTEQIEP
                     DPERLDAIRSRRQLLVELRRKYGETIEDVMEFHRRAAEQLDDLLSHEARAATLEAAIA
                     EARATEAAAAAKVAAARRKAAPKLAAAVADHLEEVALGSARVEVVVGGPDPGDEVEFR
                     MSANAGMAPQPIAKAASGGELSRTMLALHQVLSVGAPTMVFDEVDAGVGGTAATAVGR
                     ALARLASGTQVLVVTHLPQVAAYADVQLSVAKSGDGLAVSTIRQLDSDDRVIELSRML
                     SGSPDSETARRHAEELLDLAARDRVG"
     gene            2372..3502
                     /gene="steA"
                     /locus_tag="PKY13_12100"
     CDS             2372..3502
                     /gene="steA"
                     /locus_tag="PKY13_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HOT80725.1"
                     /translation="MTVASIVVTGATRTDARTKDLVKRLRVGEVAVIDHKDLDRVAAE
                     TLAESGIVAVINASESSSGRYPNEGPVIVLEAGIALLDGVGADIMDRFDDGTVVTIHG
                     DEVLIDDEVVATGVRQSLESIAEIHDRSRATLGAEFIRFVENTVEYFDHNRDLVSDDL
                     EVPDVGISFEGRHVLMVVRGHDYREDLLLLRNTGYVNEQRPILIGVDGGADALVEMGM
                     PPDIIVGDFDSVSESALRGGAKLVVHAFPNGRAPGAERLDELGLDYFTFKASGTSEDI
                     AMLMAFNQGASLIVAVGTHSSMVDFLDKGRSGMASTILTRMKVGPILVDAKGVNRLYH
                     SAVRKRDLMLLVAGALAAIIIIVIVSDPIRLVIHSIWADLTG"
     gene            3516..4553
                     /locus_tag="PKY13_12105"
     CDS             3516..4553
                     /locus_tag="PKY13_12105"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HOT80726.1"
                     /translation="MINLRFHIVSIVAIFLALAIGMFAGSTLLDRATVEVLKGRQKSL
                     DTRNADLRSENDRLRTALESQVPADEAFGTQVLPELLPDTIGERPVLLIAVRGIDEDV
                     VRALQTSIRDAGGAPLGIVWLDARVDLDQPETADQVATALGIDAAKDKVKAAVVGELA
                     QGLIAAASPIDDEPADAADPDLTTTTIESPTPDPALDVLSRLVDADLLDWESPNEGEP
                     GSRTLPSGGLDVVLLSGEGAELRPNQIVVPLVRALAARLTGVIVGEVRNPRTALEVID
                     QDGVPARGTFVDPLRDDDDLSGRLITVDDVDEPFGRLAVVLALGELPGLSTGSYGIAE
                     SASQPFPTPSG"
     gene            4561..>5762
                     /locus_tag="PKY13_12110"
     CDS             4561..>5762
                     /locus_tag="PKY13_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOT80727.1"
                     /translation="MTDLNRSARSMAILTVVSRLTGFARVVVFTAVFSKTYLANTYIS
                     ANTVPNILFELFAAGALQAVLVPTMVRLLPADGGSGEDAERVAGTVLGLLCGFLAFIG
                     VAAVAFGSQVMGLLVGDVRSASVRQAEVELGALFLWFFMPQLVFYGANVVATAVLNAR
                     NRFALPVFAPTLNNVVVIGCYLWFGAIHEGPLTLDLTAAELWILAGGTTLGVVIFCAL
                     PVAAVWRSGFRLRPHFDHRHPAVRSLLREGAWAGVFLALTQVVQVVILKVANRSAGGP
                     TVYQFAFILFTLPHALFSVPVMTTRFPEMTRAAHALDWPAYRRSVGLATRSIAYMALA
                     ATAVSLAVANPGARLLSFGKAARLAPEIASATIAFAPGIVGFGLLLFFTRALYATADT
                     KTPAVVNLG"
BASE COUNT          806 a         2102 c         1942 g          912 t
ORIGIN      
        1 gggctcgacc tcatgatcgg aatcggcggc gacgggacca tgctcgacgc ggtccggctc
       61 gcgtgccggt ccgaggcacc cgtgctgggc atcaacgtgg gccagctcgg gtacctcacg
      121 tcgatcgagc cgaagttcga gccggcagcg gggcgtaccg cgctcaagcg gttcctggcg
      181 gggggctaca tcatcgagga gcgcatgcgg atcggcgctc acatcgagcg ctccgacggc
      241 accgtcgagg cgaccgacgc cggcctcaac gagatcctgc tcgaacgcgc cgaactgggg
      301 cacaccatcc ggatcgacgt gtcgctcgac ggtgaaccgt tcacgcccta cgtcgccgac
      361 ggcgtgatga tcgccacacc gacgggctcg accgcgtacg cgttctccgc ccggggaccg
      421 atcgtcgatc cccggcaccg cgcccaggtg ctcgtgccgg tgtcacctca catgctgttc
      481 gaccgcgcgc tggtgttggc ccccgacgcg gtcgtgcgca tgacggtggc cggcggccgg
      541 ccggcgcacc tgtcggtcga cggacactcc ggcggcaccc tcgacgtcgg cgatgcggtg
      601 gtgtgcacgg ccgacccggt tcccgcccgg ttggtgcggt tcgggccgcc gtcgttccag
      661 cgggtgctca agaccaagtt cggcctgccc gaccgctgag atcgtcggtc ggcggcgccg
      721 gtcgactggc agctcggtgt cggtggcgtc gcctatcgtt gcgcccaatg ctgctcgagc
      781 tcgccgtccg tgacctcggt gtgatcgccg atctccgcct cgacttcggg gccggaatgt
      841 gcgcgctcac gggcgagacc ggtgccggca agaccatgat catcgaggcg ctggcactgc
      901 tgttgggtgg caagaccgat cccaccaggg tccgccccgg agccgaggcg agcgtggtcg
      961 agggtcggtt cgcggtgccc ggcgaggccg gtgccgacga cgacgagatc gtcctccgcc
     1021 gcgtcgttcc cgccgagggg cgctcgcggt gttacgtgaa cggcgagatg gccaccgccg
     1081 ccgggctcgc cgagatcggc ggtcggctcg tcgagatctg cggtcagcac tcccaccagc
     1141 gtctccagtc gacccgggcg cagcgcgacg ccctcgaccg gttcgggtcg atcaatctcg
     1201 ctgccctcgt cgtggcgcag tcccggcgcc gcgagctcga agcgcagctg gcagcgctcg
     1261 gaggcgacga acgggcccgg gcccgcgaaa tcgacctcct ccggttccag gtcgacgagc
     1321 tcgatgccgc ggcgatcgac gatccgtccg aggacgaacg cctcgccgcc gaggaggacc
     1381 tcctggccga cgccctggcc cacctcgagg ccggtgccag cgccctcgcc gggctccacg
     1441 acgacggtgc cgccaccgac ctcgtggccg ctgcgctcgc cgccgtcgag caccgggcgc
     1501 cgttcacggc gacagcggca cggttgcacg ggctcctcgc cgagttgacc gacgtcgtcg
     1561 atgagctgcg gaccgtgacc gagcagatcg aacccgaccc cgaacggctc gacgcgatcc
     1621 gctcccgacg acaactcctc gtcgagctgc gccgcaagta cggcgagacg atcgaggatg
     1681 tcatggagtt ccaccgtcgt gctgccgagc agctcgacga cctgttgtcc cacgaggccc
     1741 gggcggccac cctcgaggcc gccatcgccg aggcccgggc caccgaagcc gctgctgccg
     1801 ccaaggtcgc cgccgcccga cgcaaggccg cccccaagct cgccgccgcg gtggccgacc
     1861 acctcgagga ggtggcgctc gggtcggcgc gcgtcgaggt cgtcgtcggc ggccccgatc
     1921 ccggcgacga ggtcgagttc cgcatgagcg ccaacgccgg catggcccca caaccgatcg
     1981 cgaaggcggc gtcgggggga gagttgtcgc gcacgatgct ggcgctccac caggtgctca
     2041 gcgtcggcgc cccgacgatg gtgttcgacg aggtcgacgc cggtgtgggc ggcacggccg
     2101 cgaccgcggt cggccgtgcg ctcgcccggc tcgcgtcggg cacccaggtg ctggtcgtca
     2161 cccacctccc ccaggtcgcg gcctacgccg acgtccaact gtcggtggcc aagtccggcg
     2221 acggcctcgc cgtgtccacg atccgtcagc tcgacagcga cgatcgcgtc atcgaactca
     2281 gccgcatgct gtcgggctca cccgacagcg agaccgcccg gcgtcacgcc gaggagctgc
     2341 tcgacctcgc cgcccgtgac cgggtcggct gatgacggtc gcctccatcg tcgtcaccgg
     2401 agcgacgcgc accgacgccc gcacgaagga cctcgtgaag cgcctccggg tcggtgaggt
     2461 cgcagtcatc gaccacaagg acctcgaccg ggtcgcggcg gagacgcttg ccgagtcggg
     2521 catcgtcgcg gtcatcaacg cgtcggagtc gagctcgggg cgctacccca acgaggggcc
     2581 ggtgatcgtg ctcgaagccg gcatcgccct cctcgacggc gtgggcgccg acatcatgga
     2641 ccggttcgac gacggcaccg tggtgacgat ccacggtgac gaagtgctca tcgacgacga
     2701 agtggtcgcc accggcgtcc ggcagtcgct cgagtcgatc gccgagatcc acgaccggtc
     2761 gcgagcgacg ctcggcgcgg agttcatccg cttcgtcgag aacaccgtcg agtacttcga
     2821 ccacaaccgc gacctcgtca gcgacgacct cgaggtgccc gacgtcggca tctcgttcga
     2881 ggggcgtcac gtcctcatgg tggtgcgcgg ccacgactac cgcgaagacc tgctgctgct
     2941 gcgcaacacc ggatacgtca acgaacagcg cccgatcctg atcggcgtcg acggcggggc
     3001 cgacgcgctc gtcgagatgg ggatgccgcc cgacatcatc gtcggcgact tcgactcggt
     3061 gtcggaatcg gcgctgcgcg ggggagcgaa gctcgtggtg catgcctttc ccaacggtcg
     3121 cgcccccggt gcggaacggc tcgacgagct cggtctcgac tacttcacgt tcaaggcgtc
     3181 gggcacgagc gaggacatcg cgatgctcat ggcgttcaac cagggagcgt ccctgatcgt
     3241 tgccgtcggc acccactcgt cgatggtcga cttcttggac aagggccggt cgggcatggc
     3301 gtcgaccatc ctcacccgta tgaaggtggg tccgatcctc gtcgacgcca agggtgtgaa
     3361 ccggctgtac cactccgccg tgcgcaaacg tgacctgatg ctgctcgtgg ccggggcgct
     3421 ggccgccatc atcatcatcg tgatcgtctc ggaccccatc cgcctcgtca ttcactcgat
     3481 ctgggccgac ctgacaggct gaaccccacc cactcatgat caacctccgc ttccacatcg
     3541 tcagcatcgt cgccatcttc ctggcgctcg ccatcggcat gttcgccggt tcgaccctgc
     3601 tcgaccgcgc caccgtcgag gtgctcaagg gccgtcagaa gagcctcgac acccgcaacg
     3661 cggacctgcg ctcagagaac gaccgcctcc gaacggcgct cgaatcgcag gtgcccgccg
     3721 acgaggcgtt cggcacgcag gtgcttcccg aactgctccc cgacacgatc ggcgaacgcc
     3781 cggtgctcct gatcgccgtg cgggggatcg acgaggacgt ggtgcgggcg ctgcagacgt
     3841 cgatccgcga cgccggcggc gcaccgctcg gcatcgtgtg gctcgacgcg cgcgtcgacc
     3901 tcgaccagcc cgagaccgcc gaccaggtcg cgaccgcgct cgggatcgac gccgcgaagg
     3961 acaaggtcaa ggcggcggtc gtcggcgagc tcgcccaggg cctcatcgcg gcggcgagcc
     4021 ccatcgacga cgagcccgcc gacgctgccg accccgatct cacgacgacc acgatcgagt
     4081 cgcccacgcc ggacccggcg ctcgatgtcc tcagccgcct ggtcgatgcc gacctcctcg
     4141 actgggagtc ccccaacgag ggcgaaccgg gttcgcggac cttgccgagc ggcggcctcg
     4201 acgttgtgct cctctcgggc gagggcgcgg aactccggcc caatcagatc gtcgtgcccc
     4261 tcgtgcgggc cctcgcagca cggctgaccg gtgtgatcgt cggagaggta cgcaatccgc
     4321 gtacggcgct cgaggtcatc gaccaggacg gggtgcccgc acggggcacg ttcgtcgacc
     4381 cgctgcgtga cgacgacgac ctctccggcc gattgatcac cgtcgacgac gtcgatgagc
     4441 cgttcgggcg gctcgccgtg gtgctcgcgc tcggcgagct gcccggcctc tcgaccggtt
     4501 cgtacgggat cgccgaatcg gcctcccagc cgttccccac cccgtcaggc tgatccgtcg
     4561 atgaccgacc tgaaccgctc ggcgcggtcg atggccatcc tcacggtcgt gtcccgtctc
     4621 accgggttcg cccgggtcgt cgtgttcacc gcggtcttct cgaagacgta cctcgcgaac
     4681 acctacatct cggcgaacac cgtcccgaac atcctcttcg agctgttcgc cgccggcgcg
     4741 ttgcaggcgg tgctcgtgcc gacgatggtg cggctcctcc ccgccgacgg cgggtcgggc
     4801 gaggacgccg agcgggtggc gggcacggtc ctcggcctgc tgtgcgggtt cctggcgttc
     4861 atcggcgtcg ccgccgtcgc gttcggttcc caggtgatgg ggttgctcgt cggcgacgtg
     4921 cgttcggcct cggtccgaca ggccgaggtc gagctgggtg cgctgttcct ctggttcttc
     4981 atgccacagc tcgtgttcta cggcgcgaac gtcgtcgcga ccgccgtgct gaacgcccgc
     5041 aaccggttcg cgttgccggt cttcgccccg acgctgaaca acgtggtcgt catcggctgc
     5101 tacctgtggt tcggagcgat ccatgagggc cccctgacgc tcgatctgac tgcggccgag
     5161 ctctggatcc tggccggcgg caccaccctc ggtgtggtga tcttctgcgc gctgcccgtc
     5221 gctgccgtgt ggcggagcgg attccggctg cggccccatt tcgaccatcg acatcccgca
     5281 gttcgctccc tgttgcgtga gggggcgtgg gccggtgtgt tcctggcgct cacacaggtc
     5341 gtgcaggtcg tgatcctcaa ggtcgcaaac cgttcggcgg gcggtccgac cgtgtaccag
     5401 ttcgcgttca tcctgttcac gctgccccac gcgctgttct cggtccccgt gatgacgacg
     5461 cggttccccg agatgacccg cgccgcccat gccctcgact ggccggccta ccggcgaagc
     5521 gtcggtctcg cgacccgatc gatcgcgtac atggcgttgg cggcgaccgc ggtgtcgctc
     5581 gccgttgcca atcccggcgc ccggctcctg tcgttcggga aggcggcccg cctcgccccc
     5641 gagatcgcca gcgccacgat cgcgttcgcc ccgggaatcg tcggcttcgg gttgctgctg
     5701 ttcttcaccc gcgccctcta cgcgaccgcc gacaccaaga cgccggcggt cgtgaacctc
     5761 gg
//
LOCUS       DAOSOA010000211         5757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14110_length_5757_cov_4.909681, whole genome shotgun sequence.
ACCESSION   DAOSOA010000211 DAOSOA010000000
VERSION     DAOSOA010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14110_length_5757_cov_4.909681"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PKY13_12115"
     CDS             complement(<1..956)
                     /locus_tag="PKY13_12115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT80728.1"
                     /translation="MSRSADHRYGMTIPFDHVPLPEQREWVEELVDLGYTDVWSSEAN
                     GADAFTPLALASVWAPSLRLGTAIVPAYTRGPATLAQQVGTMAAAAPGRFVFGVGTSS
                     NVIVEKWNGLDFDEPYLRTRDTVRFLKDALTGAKTDMDGTGFSSHGFRLGVPVPDGTV
                     PILVAALREGMLKMAGREADGAIINWLSADDVSTVAPIVQAASPDGTPKEVVARIFVA
                     PTTDRELVHKFGRVAIAAYLNVPVYAAFHDWMGRGEMLTPMWDAWKAGDRTAALEAIP
                     DQLVDDLIVWGSPEECREHIGRYVENGVTTPALAILPFGLDQR"
     gene            complement(995..1420)
                     /gene="arfB"
                     /locus_tag="PKY13_12120"
     CDS             complement(995..1420)
                     /gene="arfB"
                     /locus_tag="PKY13_12120"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOT80729.1"
                     /translation="MVEIGEDLRLSRSVVIPAAELEWRFSASGGPGGQHANTSNTRAE
                     VVFDIASSPSLSEPIRARLVAKLGPEASAVSSEQRSQYQNRRAALQRLETMLQRAMVV
                     PRRRVATKPTLGSKKRRLEDKRQQSERKANRQRPPRDHD"
     gene            complement(1435..2376)
                     /locus_tag="PKY13_12125"
     CDS             complement(1435..2376)
                     /locus_tag="PKY13_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOT80730.1"
                     /translation="MLVVYHGEAGPRQVQRFAEVADRHGLRIEVCSPTQPERLAELLP
                     EMDAWWHVLTPITAEHLDAAPRLRIVHKWGVGVNTIDVAAATERGVAVTNMPGSNAAA
                     VAEATLLLMLSVLRQLPTFHTETVSGRGWSIPPNVGERNRELAGSTVGLVGYGDIAQR
                     LGSVLTALGATVVHHARRADRPGWLPLDDLLAMSDVVSLHVPLTPETELLLDADRIAR
                     MRPGAVLVNTARGGLVDLDALHAALDAGTLSGAGLDVFPDEPWDARHPISEHPRVVVT
                     PHVAWLTWETLERSLDLAVANVLAIRDGQPLAHVVTG"
     gene            complement(2383..3219)
                     /locus_tag="PKY13_12130"
     CDS             complement(2383..3219)
                     /locus_tag="PKY13_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT80731.1"
                     /translation="MDALEFLGLQPTHNPNRWVLPVVPGLATAGRFLFGGCALGAAIC
                     AMEEVTGRPVIWATGQYLSFAMAGEIMDVDVTIATSGHFTTQARVVGHVADREILTVN
                     AALGTRPLEATGQWAVRPDVPTPDQCEQRASRWDVGDTIMERMEIRLAKGRQWEDLDG
                     TPSADGNSALWIRMPDLAMSPATLAVLGDYVPFGIAQALGRWTRANSLDNTLRILNNR
                     PDDTGDGWVLLDIRVHGVLDGFGHGLVHLWAEDGRLLATASQSAIVRELTESGDMPAR
                     KA"
     gene            3267..4655
                     /locus_tag="PKY13_12135"
     CDS             3267..4655
                     /locus_tag="PKY13_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT80732.1"
                     /translation="MADTPWLGDTVSLVDAFRSGDRSPVEELEATLTAIETSELNAFT
                     TLDPERALAAAKQADVSQPFGGVPLGIKALTPVEGWANDRCSVALQGEVAEHTSLHVE
                     RLLDAGMVPVGQCNASEFGGLNVSVNKLYGVTHNPWQIGRTAGGSSGGSAAAVAGGLV
                     TIATGGDGGGSIRIPGAFNGLVGMKGTAGRIPRGPFTEIHPMTVVTGPMARSVRDVCR
                     WYDVTTGFDMRDPYSLPKIDGWERDLGTHSLKGKRAVIAPNLGVAVVRPEVEERVRAA
                     GEALARDAGLELVDVPVALPGLGYEWALANLSQLLRELGDAYPERKDDLTLEIAFGLH
                     IATDQFNLQMMANTEIARTNANERMAEVFDHVDFIICATNPDVAFAADTYLNQHVGGR
                     QVGPENNGALTIPANIVGNPAISVPIEQLDDLPVGMQIIGRHHEDQLLLDLAAIVERE
                     RPWPLVAPASPV"
     gene            complement(4659..5489)
                     /locus_tag="PKY13_12140"
     CDS             complement(4659..5489)
                     /locus_tag="PKY13_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80733.1"
                     /translation="MIGQRFDRSAAERILQRAIELEDDRDPSTPFEGLSEATLREAAE
                     ELGIPASAVQQAAAEERLGVLEGTDRRADRLVGPGVVTAVRIVDGRRSDVLAHTDEWL
                     RRAAFRRRRFSPSFAEYSRRGDPVAVAQRAVRSVGGHEGLGSVRQLRVTAEPIGADAT
                     VLALIVDLEPQRKLTLAGGGSVAGAGVTASAVEASLVTPWIWLGVPASVAAGYGILRA
                     RARTLPDIETALNGALDRIAAADLPPSALGGVRDQLTSRGLSRTIREQMAAVRGSARS
                     "
BASE COUNT          889 a         2019 c         2017 g          832 t
ORIGIN      
        1 cgctggtcga ggccgaacgg gaggatcgcc agggccgggg tggtgacgcc gttctccacg
       61 tagcggccga tgtgctcgcg gcactcctcg ggcgaccccc acacgatgag gtcgtcgacc
      121 agctggtcgg ggatggcttc gagcgccgcc gtgcggtcgc cggccttcca cgcgtcccac
      181 atcggcgtca gcatctcgcc gcggcccatc cagtcgtgga acgccgcgta gaccggcacg
      241 ttgaggtacg cggcgatcgc gacgcgaccg aacttgtgga cgagctcgcg atcggtggtc
      301 ggcgccacga agatgcgggc cacgacctcc ttcggcgtgc cgtcgggcga cgccgcctgc
      361 acgatgggag cgacggtcga aacatcgtcg gcggacaacc agttgatgat cgcgccgtcg
      421 gcctcgcggc ccgccatctt caacatccct tcgcgcaggg ccgcgacgag gatcggcacc
      481 gtgccgtcgg ggacgggcac gccgagccgg aatccgtggc tcgagaaccc ggtgccgtcc
      541 atgtcggtct tggcgcccgt gagggcgtcc ttcaggaagc gcacggtgtc acgggttcgc
      601 aggtagggct cgtcgaagtc cagcccgttc catttctcga cgatcacgtt cgaggacgtc
      661 ccgacaccga agacgaaccg gcccggtgcg gccgcggcca tcgtgcccac ctgctgcgcg
      721 agcgtcgccg ggccacgggt gtaggccggc acgatggccg tgccgagccg cagcgacgga
      781 gcccataccg acgccaacgc cagtggagtg aacgcatcgg cgccgttcgc ctccgacgac
      841 cacacgtcgg tgtatccgag gtcgacgagc tcctcgaccc actcccgctg ctcgggcagg
      901 ggcacgtggt cgaacgggat ggtcatgcca tatcggtgat cagcggagcg agacatgccc
      961 gaaccctaga tcggccgcgt accgcctgtt ctggtcagtc gtgatcgcgg ggagggcgct
     1021 ggcggttggc cttgcgttcc gattgctggc gcttgtcctc cagccgtcgc ttcttggaac
     1081 cgagcgtcgg cttggtggcg acccggcgtc ggggcacgac catcgcccgc tgcagcatcg
     1141 tctcgagacg ctgcaacgcc gcacgacggt tctggtactg cgaccgctgc tcggagctga
     1201 cggcactcgc ctccggcccg agcttcgcca cgaggcgcgc ccggatcggc tcggacaacg
     1261 acggtgagga cgcgatgtcg aacacgacct cggcacgggt gttcgaggtg ttggcgtgct
     1321 ggccaccggg tccgcccgag gcgctgaagc gccactcgag ttcggccgcg gggatcacca
     1381 cactgcgact caggcggagg tcctcgccga tctcgaccac gtcgcgcccg ccgctcagcc
     1441 ggtgacgacg tgggcgagcg gctgcccgtc gcggatcgcc agaacgttgg ccacggcgag
     1501 gtcgagcgag cgctcgagcg tctcccaggt cagccatgcg acgtggggcg tgacgaccac
     1561 ccggggatgc tcgctgatcg ggtgccgggc gtcccagggc tcgtcgggga acacgtcgag
     1621 accggcaccc gacagcgtcc cggcgtcgag cgccgcgtgc aacgcgtcga gatcgacgag
     1681 cccaccccga gccgtgttca cgagtaccgc acccggtcgc atgcgtgcga tccgatcggc
     1741 gtcgaggagc agctcggtct cgggcgtgag tggcacatgc aacgacacga cgtcactcat
     1801 tgcgagcagg tcgtcgaggg gcaaccaccc cggccggtcg gcacgtcgag catggtggac
     1861 gaccgttgcg ccgagcgccg tgagcaccga gccgaggcgc tgcgcgatgt cgccgtagcc
     1921 gacgaggccg accgtcgaac cggcgagctc acggttgcgc tcccccacgt tcggcgggat
     1981 cgaccagccc cggccggaga cggtctcggt gtggaaggtc gggagttgcc ggagcaccga
     2041 cagcatcagc agcagcgtcg cctcggcgac ggcagcggcg ttcgacccgg gcatgttggt
     2101 gacggccaca ccacgctcgg tcgccgcggc gacgtcgatg gtgttcacgc ccacgcccca
     2161 cttgtggacg atccgcagcc ggggcgccgc gtcgagatgc tcggcggtga tcggcgtgag
     2221 gacgtgccac cacgcgtcca tctccggcag cagctcggcg agccgctccg gttgcgtcgg
     2281 cgaacacacc tcgatgcgca ggccgtgccg gtcggcgacc tcggcgaagc gctggacctg
     2341 acgggggccc gcctcgccgt ggtagacgac gagcaccgtc ggtcaggcct tgcgagccgg
     2401 catgtcaccg gactcggtga gctcacgcac gatggcggac tggctggcgg tggcgagcag
     2461 ccgaccgtcc tcggcccaca ggtggacgag gccgtggccg aacccgtcga gcaccccgtg
     2521 cacacggatg tcgagcagca cccacccatc gccggtgtcg tccggacggt tgttgaggat
     2581 gcgaagggtg ttgtcgaggc tgttggcccg ggtccaacgg ccgagcgcct gggcgatgcc
     2641 gaacggcacg tagtcgccga gcaccgccag cgtcgccggc gacatcgcaa gatccggcat
     2701 gcggatccac agggcggagt tgccgtcggc ggacggcgtg ccgtcgaggt cctcccactg
     2761 ccgccccttg gccaggcgga tctccatgcg ctccatgatc gtgtcgccga cgtcccatcg
     2821 cgacgcccgc tgttcgcact ggtcgggcgt cggcacgtcg gggcggaccg cccactgccc
     2881 ggtcgcttcg agcggtcggg tgccgagcgc ggcgttcacc gtgaggatct cgcgatcggc
     2941 cacgtggccg acgacgcgag cctgcgtcgt gaagtggccc gaggtggcga tcgtgacgtc
     3001 gacgtccatg atctcgccgg ccatcgcgaa cgagaggtac tgccccgtcg cccagatgac
     3061 cggacgaccc gtgacctctt ccatcgcgca gatcgcggcg ccgagcgcac agccaccgaa
     3121 gaggaaccgg ccggccgtcg ccagccctgg caccaccggg aggacccaac gattggggtt
     3181 gtgagtcggc tgcaatccga ggaactcgag ggcatccatc gaggatcagg ctaggggatg
     3241 cggaacaacc tctacgctct cctgccatgg ctgacacacc ctggttgggc gacaccgttt
     3301 cactcgtcga cgcgttccgt tcgggcgacc gatcaccggt cgaggagctc gaggcaaccc
     3361 tgacggcgat cgagaccagc gagctgaacg cgttcacgac cctcgacccc gagcgggcac
     3421 tggcggcagc gaagcaggcc gacgtctccc agccgttcgg cggggtgccg ctcggcatca
     3481 aggcgctcac gccggtcgag gggtgggcca acgaccgctg ttcggtggcc ctccagggcg
     3541 aggtcgccga gcacaccagc ctccacgtcg aacgcctcct cgatgccggc atggtgccgg
     3601 tcgggcagtg caacgccagc gagttcggcg gtctcaacgt cagcgtcaac aagctgtacg
     3661 gcgtcacgca caacccgtgg cagatcggcc ggaccgccgg tggctcgtcg ggtggttcag
     3721 ccgccgccgt cgccgggggc ctcgtcacga tcgccaccgg cggtgacggc ggtggctcga
     3781 tccgcatccc gggcgcgttc aacggcctcg tcggcatgaa gggcaccgcc gggcgcatcc
     3841 cccggggtcc gttcaccgag atccatccga tgacggtcgt caccggcccg atggcccgtt
     3901 cggtgcgcga cgtgtgccgc tggtacgacg tcacgaccgg cttcgacatg cgcgatccct
     3961 actcgctccc gaaaatcgac ggatgggaac gcgacctcgg aactcattcc ctcaagggca
     4021 aacgtgcggt gatcgccccg aacctgggcg tcgctgtcgt acgacccgag gtggaggagc
     4081 gggtccgcgc cgccggcgag gccctggccc gcgacgccgg cctcgaactc gtcgacgtgc
     4141 cggtcgcgct tccgggcctg ggctacgaat gggcgctcgc caacctgtcg cagctgctcc
     4201 gagagctcgg cgacgcgtat cccgagcgca aggacgacct gaccctcgag atcgcgttcg
     4261 gcctgcacat cgcgaccgat cagttcaacc tgcagatgat ggccaacacc gagatcgccc
     4321 gcaccaacgc caacgagcgg atggccgagg tgttcgacca cgtcgacttc atcatctgcg
     4381 ccaccaaccc cgacgtcgcg ttcgccgccg acacctacct caaccagcac gtcggcggcc
     4441 gccaggtggg ccccgagaac aacggggcac tcaccatccc ggccaacatc gtcggcaacc
     4501 cggcgatctc ggtgccgatc gagcagctcg acgacctgcc ggtgggcatg cagatcatcg
     4561 gccgtcatca cgaggaccag ctgttgttgg atctcgcggc gatcgtcgag cgcgagcgtc
     4621 cctggccgct cgtcgcgcct gcgtcgcccg tctgaggttc aggagcgggc cgagccccgc
     4681 acggccgcca tctgctctcg gatcgtgcgg ctcagacccc gtgaggtgag ctggtcgcgc
     4741 acaccaccga gcgcgctcgg cggtagatcg gccgcagcga tgcggtcgag ggccccgttg
     4801 agcgcggtct cgatgtcggg gagcgtccgg gcacgggcgc ggagaatgcc gtagccggca
     4861 gcgacggacg ccggcacccc gagccagatc cacggggtga cgagcgacgc ctcgacggcg
     4921 gatgccgtga cgcccgcgcc ggcgaccgag ccgcccccgg ccagcgtcag cttgcgctgg
     4981 ggctcgaggt cgacgatgag ggcgagcacg gtggcgtcgg cgccgatcgg ttcggcggtg
     5041 acccgcagct gacgaaccga accgagcccc tcgtggccac cgacggaccg aacggcccgc
     5101 tgggccacgg cgaccgggtc gccccgccgt gagtactcgg cgaacgatgg ggagaagcgg
     5161 cgccggcgaa acgctgcccg gcgaagccac tcgtcggtgt gggcgaggac gtcggagcgc
     5221 cggccgtcga cgatccgcac ggccgtgacg acgccaggcc cgaccagccg gtcggcccga
     5281 cgatcagtgc cctcgaggac gccgagtcgc tcctcggcgg cggcctgttg caccgcggac
     5341 gccggaatgc ccagttcctc ggccgcttcg cgcagcgtcg cctcggagag cccctcgaag
     5401 ggagtcgacg ggtcacggtc gtcctcgagt tcgatggcgc gctgcagaat ccgctctgct
     5461 gccgaccgat cgaaccgttg cccaatcacg ggttcagcct aggggacccc actgaggctg
     5521 ggtccgcggg tcatgcgccg cgtggtgcgc cggatctgat ggtgccgtgg tgttgacccc
     5581 agaggttgat accggacggg gtcgtgatcc agcaccatct gtcgtcgccg atgtggaggc
     5641 cccagccctt tcgatggatc aggcgtcggt gtcgcactgt catccgatcc gaggccgagt
     5701 cgacgccgac actctcgatc gcgcagatgg agtcctactg cacttcagcg atcgaga
//
LOCUS       DAOSOA010000212         5747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14139_length_5747_cov_8.905130, whole genome shotgun sequence.
ACCESSION   DAOSOA010000212 DAOSOA010000000
VERSION     DAOSOA010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14139_length_5747_cov_8.905130"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1404
                     /locus_tag="PKY13_12145"
     CDS             118..1404
                     /locus_tag="PKY13_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOT80734.1"
                     /translation="MRNRIVWERACGLTRTVVEAVDVDEVIGAVIVSARPEAKARQRC
                     GQCGRRCPKFDRGEGRRRWRALDAGTMKVFIEADAPRVRCRDHGVTVAQVPWARHGAG
                     HTRDFDDQAAWLAVHASKSAVVELLRVAWRTVGSIIARVNADIDANFDRFAGLERIGI
                     DEISYKRGHRFLIVVVDHDTGRLVWAAPGRDEATLHRFFDQLGEDRAAHITHVSADMA
                     SWIARVVAQRAPNAVRSADPFHVVAWASDALDVERRLAWNQAAGRRKVDPSSGRRKDA
                     TGPARAVKRSRYALWKNPENLTPRQREQLDWIAKTDPRLWRAYLLKEGLRYVFAAKGE
                     AGRQALDRWISWARRSRLPAFVDLQRRVVKHRAAIEATLDSGLSNALVESTNTKIRLL
                     TRVAFGFHGPEPLIALAMLALGGHTPDLPGRRMTHG"
     gene            complement(1432..1926)
                     /locus_tag="PKY13_12150"
     CDS             complement(1432..1926)
                     /locus_tag="PKY13_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HOT80735.1"
                     /translation="MTDMTAATVTTADTQDAPRYLEPGWFTRNVFNRLMARLAKWGVS
                     VWGSRQLAVRGRASGEWKTTPVNPLDFEGGRYLVAPRGTTQWVRNIRVAGTAELRLGR
                     KVEPIAVVELADADKPAVIREYLRRWKAEVGQFFDGINERSTDDELLAVAAGFPVFRI
                     LPAP"
     gene            2061..2765
                     /locus_tag="PKY13_12155"
     CDS             2061..2765
                     /locus_tag="PKY13_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT80736.1"
                     /translation="MTTKAPTARERARAEITAEILTVARRHLAEGGAASLSLRAVARE
                     VGMVSSAVYRYFPSRDELLTRLIVDAYTAVADAAEAADAAVDRGDLVGRIVGAATAIR
                     RWAVAHPHEYALVYGTPVPGYAAPQDTIDPALRVTLVFTSVLVDGMAAGDVAAEDRIE
                     TTRGVRKDFATIRSVAPGVPDGVLSRGLILLTQLFGHISYELFGHLHNGITDYDGFFD
                     LQVRRLAAYVIDGPGT"
     gene            complement(2859..3392)
                     /locus_tag="PKY13_12160"
     CDS             complement(2859..3392)
                     /locus_tag="PKY13_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT80737.1"
                     /translation="MTDTTPTTPTTGITGEHTIDGRPHGFSSVTPFLVVPGAAAALDF
                     YRDVLGATIGGVTEMPGPDGDVIVVHAEVRFSDGRIQIGDPNPTYGLVPPVGDGHTVS
                     YSMGLYVTDVDAVVDRATAAGAVVREEAQTFVSGDRFASIIDPFGIRWSLMTRVEDLS
                     EEESNARVAEWAASQTA"
     gene            3475..>4252
                     /locus_tag="PKY13_12165"
     CDS             3475..>4252
                     /locus_tag="PKY13_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT80738.1"
                     /translation="MTLAKKGVLRPDESLATVDRRELAPRFADLARQVWVPQWRLPDG
                     VTFDQPVLQYPAVNVAVKAGTAVAYLPVDGVGVRTLTGSGWAAGVLLRPGAGALVVPG
                     DHAELLGAEVPIADDALVSAVAAAMADAIDLDAVVAAYETWLTPLLDRVDDETVLVNA
                     VCDAAEHDTTITTPRELYERFDLSERSMQRLLKRRVGFSPRWLIQRRRLQEAAFRIRE
                     EPEVALAELAADLGYADQAHFTRDFGKVVGQPPGDYRRSLG"
     assembly_gap    4253..4352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4400..>5747)
                     /locus_tag="PKY13_12170"
     CDS             complement(4400..>5747)
                     /locus_tag="PKY13_12170"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOT80739.1"
                     /translation="LRGGSGLGGGVVTTPAEVGADRRSRQRIAPVHITDYADPQFSPE
                     VAAMIEAAKPLADLLPLEVDAICDQAAAELSASGRQFSDFGEVDGVESFRVRMAALLD
                     AYAAVPDLSHMGRITVHTLFTQLVRNRMLIADLLARHPEIHEIEITAPIIIAGLPRTG
                     TTHLHNLLSADPGLRSLPYWESTEPVSLPTDSIGPDGVDPRWTRTAAACDFMEAAMPY
                     FKRMHEMTPDHVHEEIQLLAIDFSSMFFETLAPVPAWRDRFLATDQVPHYEYLKTVLK
                     VLTFLRGGRRWVLKSPQHLEQYPAIRQVFPDATMVVTHRDPVAVTASMVTMIAYTQRM
                     QHERVDPHAVGALWSDRLQRMLGSAVAERDLLPEAQSMDVRFDEFMADDLATVAAIYE
                     LADQPLDDRARAAHAGYLASHRRERHGGLAYDLADFGLDATELARGFADYTTRFLS"
BASE COUNT          894 a         1932 c         1946 g          875 t
ORIGIN      
        1 acaaaaccgg ctctcctgac ttatccgtgg gtgtgatctg aggccatccg cggttgcacc
       61 ccgcctgctt cctacgtttc gggctgtcga aggtcctaga cggggaggca gtgcggggtg
      121 cggaacagga tcgtatggga gcgggcgtgt ggtttgacgc gcacggtcgt cgaggccgtc
      181 gatgttgatg aggtgattgg ggcggtgatc gtgtccgctc ggcccgaagc caaggcgcgg
      241 caacggtgcg gacagtgcgg tcgtcgctgc ccgaagttcg atcggggtga agggcgtcgc
      301 cggtggcgag cgctggacgc tggaaccatg aaggtgttca tcgaggcaga cgcaccgcga
      361 gtgcgatgcc gtgatcacgg agtcactgtc gctcaggttc cgtgggcccg acacggcgct
      421 gggcacaccc gcgactttga cgatcaggcc gcgtggctcg cggtccatgc ttccaagtcg
      481 gcggttgttg agttgttgcg ggtggcgtgg cgaactgtcg gatcgatcat cgctcgcgtc
      541 aacgctgaca tcgacgcgaa cttcgaccgg ttcgccggcc tggaacggat cgggatcgac
      601 gagatctcct acaagcgtgg ccaccggttc ctgatcgtgg tcgtcgatca cgacaccggt
      661 cgtctcgtgt gggcggcccc gggccgtgat gaagcgacgt tgcaccggtt cttcgaccaa
      721 ctcggcgaag accgggccgc gcacatcacc cacgtgtcgg ccgatatggc gagctggatc
      781 gcccgagtcg tcgctcagcg ggctccgaat gcggtgcggt ccgcggaccc gttccacgtc
      841 gtggcgtggg cgagcgacgc gctcgatgtc gaacggcgtc tcgcttggaa ccaggcagcg
      901 ggacgccgca aagtcgaccc gagtagcggc cgccgcaaag acgccaccgg gccagcccgt
      961 gcagtgaaac ggtcgcgcta cgcgctctgg aagaaccccg agaacctgac tccacgccaa
     1021 cgagaacaac tcgactggat cgccaagacc gacccgcggt tgtggcgggc gtacctgttg
     1081 aaagaagggc tccgctacgt gttcgccgcg aaaggcgagg ccggcagaca agccctggac
     1141 cgatggatct cgtgggcccg ccgatcccgg ctccccgcat tcgtggatct ccaacgccgc
     1201 gtcgtcaaac accgagccgc gatcgaggcg accctcgact cgggcctgtc caacgcactc
     1261 gtcgagtcca ccaacaccaa gatccggcta ctcacccgtg tcgcgttcgg gttccacggc
     1321 cccgaaccac tcatcgccct cgccatgctc gccctcggag gacacacacc cgacctcccc
     1381 ggccgccgaa tgacccacgg ataagtcagg agagccacaa aaccccacgg ctcagggcgc
     1441 cgggaggatg cggaagacgg ggaagccggc ggcgacggcg aggagttcgt cgtcagtgga
     1501 acgctcgttg atgccgtcga agaactggcc gacctcagcc ttccagcggc ggaggtactc
     1561 acggatgacc gccggcttgt cggcgtcggc cagctcgacc acggcgatgg gctcgacctt
     1621 ccggccgagg cgcaactcag ccgtgccggc gacacggatg ttgcggaccc attgggtcgt
     1681 gccacggggg gcgacgaggt aacgaccgcc ctcgaagtcg agcgggttga ccggcgtggt
     1741 cttccactcg ccactcgcgc ggccccgcac ggccagctga cgggaacccc acacactcac
     1801 accccacttc gcgaggcgcg ccatcaggcg gttgaacacg ttgcgggtga accagcccgg
     1861 ctcgaggtag cgcggtgcgt cctgcgtgtc ggccgtggtg acggttgctg cggtcatgtc
     1921 ggtcatgtcg gtgtcctccg agaagagaga gcactgctct cgtttcgatg tgaccatcat
     1981 gcgccggcag ttccgacgtt gtcaagagca ctgctctcgt tttgttgcgc cgctctcgca
     2041 tgacgtgcag actgaccgtc atgacgacca aggcacccac ggcccgcgag cgcgcccgcg
     2101 ccgagatcac cgccgagatc ctgacggtgg cgcgccgcca cctcgccgag ggcggtgccg
     2161 cgtcgctctc gttgcgcgcc gtcgcccgag aggtgggcat ggtgtcctcc gcggtctacc
     2221 ggtacttccc cagccgcgac gaactcctga cgcgtctcat cgtcgacgcc tacaccgcgg
     2281 tcgcggacgc ggcggaggcc gccgacgctg ccgtcgaccg aggggatctc gtcggtcgaa
     2341 tcgtcggcgc cgccaccgcg atccgccggt gggcggtggc ccacccgcac gaatacgcgc
     2401 tcgtctacgg caccccggtg ccggggtacg cggcaccaca ggacaccatc gacccggccc
     2461 tgcgggtcac gctggtgttc acgtccgtcc tcgtcgacgg catggccgcc ggcgacgtgg
     2521 ccgccgagga ccgcatcgag acgacacgcg gcgtccgcaa ggacttcgcc accatccggt
     2581 cggtcgcgcc gggagttccc gacggggtcc tctcacgcgg cctgatcctg ctgacacagc
     2641 tcttcggcca catcagctac gagctgttcg gccacctgca caacggcatc accgactacg
     2701 acgggttctt cgacctgcag gtccggcgcc tggccgccta cgtgattgac ggccccggca
     2761 cctgaccggt tctgaccgat tctgtcccgc gagggcctgc aatctgcacg ccctcgtggg
     2821 acaagacgag cagcgcggga cagaaccagc ggggcgggtc aggcggtctg gctggcggcc
     2881 cactcggcga cgcgggcgtt gctctcctcc tcggagagat cctcgacgcg ggtcatgagc
     2941 gaccagcgga tcccgaaggg atcgatgatc gaggcgaacc ggtcgccgga cacgaacgtc
     3001 tgtgcctctt cgcgcaccac ggcaccggcg gcagtggccc gatcgaccac ggcgtcgacg
     3061 tcggtgacgt agagacccat cgagtagctg accgtgtggc catcgcccac cggcggcacc
     3121 aggccgtagg tcgggttcgg gtcgccgatc tggatgcgcc cgtcggagaa ccgtacctcg
     3181 gcgtggacga cgatcacgtc gccgtcgggg ccgggcatct cggtgacacc accgatcgtg
     3241 gcgccgagca cgtcacggta gaagtcgagc gctgcggcgg cacccggcac gacgaggaac
     3301 ggggtgacgc tgctgaaccc gtgcgggcgc ccgtcgatcg tgtgctcgcc ggtgatacca
     3361 gtggtggggg tcgtgggggt ggtgtcggtc atggcgatca cggtacggac atccaccggt
     3421 cgaggtcttg aacgttcccg acagcccaca gcgccccggc gtatcctccg caggatgacg
     3481 ctggcgaaga agggggtgct gcgccccgac gagtcgctgg cgaccgtcga ccgacgcgag
     3541 ctcgctcctc gcttcgccga cctcgcccgg caggtgtggg ttccgcagtg gcggctcccc
     3601 gacggggtga ccttcgacca gccggtgctc cagtaccccg ccgtcaacgt ggccgtcaaa
     3661 gcggggaccg ccgtcgccta cctgccggtc gacggcgtgg gcgtccgaac gctgaccggc
     3721 agcgggtggg cggccggcgt gctgctccgg cccggcgccg gcgcgctcgt cgtgcccggc
     3781 gaccacgccg agctcctcgg tgccgaagtg ccgatcgccg acgacgcgct cgtctccgcc
     3841 gtcgccgcgg ccatggcgga cgccatcgat ctcgacgccg tcgtcgccgc gtacgagacg
     3901 tggctgactc ccctgctcga ccgcgtcgat gacgagaccg tgttggtgaa cgccgtctgc
     3961 gacgcggcgg aacacgacac gacgatcacg acgccccgcg agctgtacga gcggttcgat
     4021 ctctccgaac gctcgatgca gcgcctcctg aaacggcgcg tcgggttctc accgaggtgg
     4081 ctcattcagc gccgccggtt gcaggaagcc gcgttccgca tccgggagga acccgaggtg
     4141 gcgctcgccg agttggccgc cgatctcggc tacgccgacc aggcccactt cacccgcgac
     4201 ttcggcaagg tcgtgggcca acctccgggc gactaccgcc ggtccctcgg ggnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtagtgcg aggagcgaca ctcgcgtcgc
     4381 tcctcgcact acgaaacccc tagctcagga agcgggtcgt gtagtcggcg aagccccggg
     4441 ccagttcggt ggcgtcgagg ccgaagtcgg cgaggtcgta ggccaggccc ccgtggcgct
     4501 cccgccggtg cgacgccagg tagccggcgt gtgcggcgcg ggcccggtcg tcgagcggct
     4561 ggtcggccaa ctcgtagatc gccgcgacgg tcgcgaggtc gtcggccatg aactcgtcga
     4621 agcgaacgtc catgctctgc gcctccggca acagatcccg ctctgcgacc gcggaaccga
     4681 gcatccgttg caggcgatcc gaccacagcg cgcccaccgc atgggggtcg acgcgctcgt
     4741 gctgcatgcg ctgggtatag gcgatcatcg tgaccatcga ggccgtgaca gcgaccggat
     4801 cgcggtgcgt caccaccatc gtcgcgtcgg ggaacacctg gcggatcgcc gggtactgct
     4861 cgaggtgctg cggcgacttc aggacccacc gccgacctcc ccgcaggaac gtcaacacct
     4921 tcagcacggt cttcaggtac tcgtagtgcg gcacctggtc agtggcgagg aagcggtcgc
     4981 gccatgccgg caccggcgcc aacgtctcga agaacatcga cgagaagtcg attgccagga
     5041 gttggatctc ctcgtgcacg tggtcgggcg tcatctcgtg catgcgcttg aagtacggca
     5101 tcgccgcctc catgaagtcg cacgccgcgg ccgtgcgtgt ccagcgaggg tcgacaccgt
     5161 cggggccgat cgagtcggtg ggcagcgaca cgggctcggt gctctcccag tagggcaacg
     5221 accgcaggcc ggggtcggcc gacagcaggt tgtgcaggtg cgtcgtaccc gtgcgaggca
     5281 gcccggcgat gatgatcggc gccgtgatct cgatctcgtg gatctccggg tgccgcgcca
     5341 gcaggtcggc gatcaacatc cggttgcgga ccagctgcgt gaacagggtg tgcacggtga
     5401 tgcgacccat gtgcgacagg tcgggcactg ccgcgtaggc gtcgagcagc gcggccattc
     5461 ggacccggaa gctctcgacg ccatcgacct cgccgaaatc ggagaactgc cggccactcg
     5521 ccgagagctc cgcggccgcc tggtcgcaga tggcgtcgac ctcgagcggc aacaggtcgg
     5581 cgagcggctt ggcggcctcg atcatcgccg cgacctcagg gctgaactgc gggtccgcgt
     5641 agtcggtgat gtgaaccggg gcgatgcgct gtcgcgagcg gcggtccgcc ccgacttcgg
     5701 ccggggtcgt cacgacacca cctccgagac cggaaccacc tcgcagg
//
LOCUS       DAOSOA010000213         5736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14176_length_5736_cov_6.443760, whole genome shotgun sequence.
ACCESSION   DAOSOA010000213 DAOSOA010000000
VERSION     DAOSOA010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14176_length_5736_cov_6.443760"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1239)
                     /locus_tag="PKY13_12175"
     CDS             complement(7..1239)
                     /locus_tag="PKY13_12175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOT80740.1"
                     /translation="MAKPAPLAGVRIIECSILGPGALTTHLVDLGAEVIKIESPAGDY
                     IRRMTWPIVEGVSLMHLHINRGKRSLVLDLRTPEAQEVFRELAKTADAVIEGMRPGAL
                     AKRGLGYEDLKAINPKIVFCNISGYGMTGPYKDMPSHGVAYDTWGGLVKAETDDEGFC
                     FIPEHPSTGIHAGPLFGAFGVVSAILGARATGEGSALEIAQSDASAAMDWLRSETWKA
                     YERPEDEVTGNPSDNYERRAPGTAGMRDGVRYQFYVTKDDRHVLFMASEQEFWKNFCD
                     GVERPDLFETWPGSQYGDHAKGNRELQAILRDIFLTKTAAEWIVFGDEKNTPIAPVNT
                     PQTIAEDPQFQDRFHWMPADEVGADMLGTPLKFMDVDMPKMTKAPTVGQHTVDVLESV
                     LGYDEAKIAALRETGAIG"
     gene            1399..1896
                     /locus_tag="PKY13_12180"
     CDS             1399..1896
                     /locus_tag="PKY13_12180"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HOT80741.1"
                     /translation="MQIVKKHTFDAPIADCWKMFSDPKAHIAKFEGMGHHGVTIVDKK
                     KTKKNLNLTVTREVDVDGIPSFAQKFIKPRNTVVSIDDWNDFGDGTFGGTFTLDTHGV
                     PVDIKGQTLLVANGDDKSDYTVTIDIKVSVPLIGGKLENFSKGIVNAQMDEEFRLGDA
                     WLAEH"
     gene            complement(1901..2830)
                     /locus_tag="PKY13_12185"
     CDS             complement(1901..2830)
                     /locus_tag="PKY13_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80742.1"
                     /translation="MSLPAIPLPPAQSYTVSADAIEALGCDECERGGTPDAARFFYGT
                     LPLGAVGDLVEGSAADTRVALGNLFASGYFGGIYLRGNLSGGLADDLSPFAPLLDQVG
                     ALTQVGLDQIVGELTRIARSGTEQEVRDATTIWSAVLAAIAGYNRGYLEVALQHPPAG
                     VTVDPGAFSCASLFDCRSTQLPMPALDALAGQRDALNGPPSFQWLTVAMAANSIGSAA
                     IPAGRDVWTGLLGSASFDAAGYRTIIDLSVGFLEVTQAALLANLAGPTGGDLALARRG
                     LDATAGLVTWAGSYFLGLASSLPNSSLPTLTCS"
     gene            3004..4188
                     /locus_tag="PKY13_12190"
     CDS             3004..4188
                     /locus_tag="PKY13_12190"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C1-like protein"
                     /protein_id="HOT80743.1"
                     /translation="MKRTVVLLAALLTLTSAACSSTEDDSAVTGSTTTTSGTSASNAL
                     TLNELQLIGTHNSYHIAPEAKLFEAEKAVAASLGEDAGGLGDIDSLAYTHQPLTDQLE
                     SGIRSFELDAYADPDGGLYATPLAPSFLGITDTPLPTGMDEPGFKVLHIADVDFMSTC
                     ATLVVCLTEIRTWSDAHPDHVPIVINLELKADGLPASLNATVPAPIDATQLDALDAEI
                     RSVFEEDRLLTPDDVRGDAATLREAVTTTGWPKLGDVRGQVMLFMDNGGDLHDLYLTG
                     HPSLEGRALFTSAAEDGDDDEAVVKVNDPAEAPRITRLVNDGFFVRTRADADLTEAQT
                     NDTTDREITLRSGAQVVSTDFPIAEPAANGYVVAFPAGPQVRCNPVLVTACPTDSLEP
                     AG"
     gene            complement(4196..5035)
                     /locus_tag="PKY13_12195"
     CDS             complement(4196..5035)
                     /locus_tag="PKY13_12195"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT80744.1"
                     /translation="METAPSEGVPARVDPDVVMLTGRVAVVTGAAQGIGAATAIALAR
                     FGAHVAVVDREADGLAGTVAEVEALGRSCVSVVGDVRRQDVVDELVAATVAMHGRIDI
                     LVNNAGGGFWASFADINAKGEAALIAENFTQVTSCIRTSLPHMTNGGSVVNVTSIEAH
                     RAGPGFGIYSAMKAAVENLSKTLALELAPQGVRVNCVAPDMIPTPGDAGLVEASGAML
                     DAAYDPTPTGRMGSVEDAAAAIVFLAGDMARFVTGTTLHLDGGTFAASGWRRRTGTSS
                     YVL"
     gene            5076..>5736
                     /locus_tag="PKY13_12200"
     CDS             5076..>5736
                     /locus_tag="PKY13_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80745.1"
                     /translation="MRESADPRLGEIESLLIEPQRDPHVDMVLSSPGPDRYRVASPLG
                     SVEFVRLIDTGTDDDGRPVDRYRYEVDAVVDADPIADTTDDRFVGLALEREGVFPTRD
                     VNAYPHAHDQIAQFFDSPHAPDLVVQHTAGHHYETNLGEHGSLGVVQARAPFIASGAG
                     VPARGTLAQSARMVDVAPTVLHLLGGAIHPEGVGPTGSRRSGALLARQDGDALTSIVD
                     PA"
BASE COUNT          967 a         2052 c         1884 g          833 t
ORIGIN      
        1 ggccgactac ccgatggcgc cggtctcgcg cagcgcggcg atcttggcct cgtcgtagcc
       61 gagcaccgac tccaacacgt cgaccgtgtg ctgaccgaca gtcggggcct tcgtcatctt
      121 gggcatgtcg acgtccatga acttgagcgg ggtgcccaac atgtcggcac cgacctcgtc
      181 ggcgggcatc cagtggaagc ggtcctggaa ctgggggtcc tcggcgatcg tctgcggcgt
      241 gttgacggga gcaatcgggg tgttcttctc gtcgccgaag acgatccact cggcggccgt
      301 cttcgtcagg aagatgtcgc gcaggatggc ctgcagctcg cggttgccct tggcgtggtc
      361 gccgtactgg ctgcccggcc acgtctcgaa caggtcggga cgctcgacgc cgtcacagaa
      421 gttcttccag aactcctgct ccgacgccat gaacagcacg tggcgatcgt ccttggtcac
      481 gtagaactgg tagcgcacgc cgtcgcgcat gccggccgtg ccgggggcgc ggcgttcgta
      541 gttgtcgctg gggttgccgg tgacctcgtc ctcgggacgt tcgtaggcct tccaggtctc
      601 gctgcgcagc cagtccatcg cggcggacgc atcggactgg gcgatctcga gggccgagcc
      661 ctcgccggtg gcgcgtgcgc ccaagatggc ggacaccaca ccgaacgcac cgaacagcgg
      721 gccggcgtgg atgccggtgg acgggtgctc ggggatgaag cagaagccct cgtcgtcggt
      781 ctccgccttc acgaggccac cccacgtgtc gtaagcaaca ccgtggctgg gcatgtcctt
      841 gtaggggccg gtcatgccgt agccggagat gttgcagaac acgatcttcg ggttgatcgc
      901 cttgaggtcc tcgtagccga ggccacgctt ggccagcgca ccgggtcgca tgccctcgat
      961 gacggcgtcg gccgtcttcg ccagctcacg gaagacctct tgggcctccg gcgtgcgcag
     1021 gtcgagcacc aacgatcgct tgccccggtt gatgtggagg tgcatgaggg agacgccctc
     1081 gacgatcggc caggtcatgc ggcggatgta gtcgccggcc ggcgactcga tcttgatcac
     1141 ttcagctccg agatcgacca ggtgcgtggt gagggcgccc gggccgagga tcgaacactc
     1201 gatgatgcgg actcctgcga ggggggctgg cttggccatg gatgggatct cccgatcgat
     1261 caacggtttc tgacggaccg tcagattcta ggtggcgtcc cgatcccacg cccaacggac
     1321 gcagcagtcg ggcaggcacc gccgcgaccg ggcagaacac cccgtgcccc gatccggtgg
     1381 cgatgcttga atgaccgcat gcagatcgtc aagaagcaca cgttcgacgc cccgatcgcc
     1441 gattgctgga agatgttcag cgatccgaag gcgcacatcg ccaagttcga gggcatggga
     1501 caccacggcg tgacgatcgt cgacaagaag aagacgaaga agaacctgaa cctcacggtc
     1561 acgcgcgagg tcgacgtcga cggcatcccg agcttcgccc agaagttcat caagccgcgc
     1621 aacaccgtcg tgtcgatcga cgactggaac gacttcggcg atggcacctt cggcggcacg
     1681 ttcaccctcg acacccacgg agtgccggtc gacatcaagg gccagacgct cctcgtcgcc
     1741 aatggcgacg acaagtccga ctacacggtc acgatcgaca tcaaggtgtc ggtgccgctc
     1801 atcggcggca agctcgagaa cttctcgaag ggcatcgtca acgcccagat ggacgaggag
     1861 ttccgactcg gcgacgcctg gctcgccgag cactgacggg tcaggaacag gtcagcgtcg
     1921 gcagcgacga gttcgggagc gacgacgcga gcccgaggaa gtagctgcca gcccacgtca
     1981 ccagaccggc ggtcgcgtcg agcccgcggc gagccaacgc caggtcgccg ccagtgggcc
     2041 cggcgaggtt ggccagcagg gcagcctggg tgacctcgag gaatcccacc gacaggtcga
     2101 tgatcgtgcg atacccggcg gcatcgaacg aggccgaccc gaggaggccg gtccacacgt
     2161 cacggccggc gggaatcgct gcgctgccga tcgagttcgc ggccatcgca acggtcaacc
     2221 actggaacga gggcgggccg ttgagcgcgt cacgctggcc ggcgagagcg tcgagcgccg
     2281 gcatcggcag ctgcgtgctc cggcagtcga acagcgaggc gcaggagaac gcgcccggat
     2341 ccacggtcac tccggccggc gggtgctgca gggcaacttc gaggtagccg cggttgtagc
     2401 cggcgatggc ggcgagcacg gccgaccaga tcgtcgtcgc gtcccggacc tcctgctcgg
     2461 tgcccgaccg ggcgatgcgg gtgagctctc cgacgatctg gtcgagaccg acctgggtga
     2521 gcgccccgac ctgatcgagc agcggggcga acggggacag gtcgtcggcg agtccacccg
     2581 acaggttgcc ccgcaggtag atgccgccga agtagccgga cgcgaacagg ttcccgagcg
     2641 cgacccgcgt gtcggcggcc gacccctcga cgagatcgcc gaccgctccg agcggcaacg
     2701 tgccgtagaa gaaccgcgcc gcatcggggg tgccgccgcg ctcgcactcg tcgcagccga
     2761 gcgcttcgat cgcatcggcg gacaccgtgt aggactgcgc cggtggcaag gggatggcag
     2821 gcaggctcac acgcgtgacg ggaccgccgg aggacggaac ggtcgtgttc gaccgcggac
     2881 ccggcgaacc cgagggcgcg cacgccgcca atcccacgag agccccgacc aggcagatcc
     2941 cggccatgcg tcgcgtcctt cgcaccatct cgttcacctt tcgttcccgc tacgtttccc
     3001 cagatgaaac gcaccgtcgt gttgctcgcc gcactcctga cgctcacgag cgctgcctgt
     3061 tcctcgaccg aggacgacag cgccgtgacc ggcagcacga cgacgacgtc aggtacctcg
     3121 gcgtcgaacg ctttgacctt gaacgaactc caactgatcg ggacgcacaa cagctaccac
     3181 atcgcccccg aggcgaagct gttcgaggcg gagaaggccg tcgccgcgag cctcggcgag
     3241 gacgccggcg gcctcggcga catcgactcg ctggcgtaca cccaccagcc actcaccgac
     3301 cagctcgaga gcggcatccg cagtttcgag ctcgacgcct acgccgatcc cgacggcggt
     3361 ctctacgcca caccgctcgc gccctcgttc ctcggcatca ccgacacacc gctgcccacc
     3421 ggcatggacg agcccggctt caaggtcctc cacatcgccg acgtcgactt catgtcgacg
     3481 tgcgcgaccc tcgtggtgtg cctgaccgag atccgcacct ggtccgatgc ccaccccgac
     3541 cacgtcccga tcgtcatcaa cctcgagctc aaggccgacg gcctccccgc gtcactcaac
     3601 gccacggtcc ccgcccccat cgatgcgacc cagctcgacg cgctcgacgc cgagatccgc
     3661 tcggtgttcg aggaggaccg gctcctgacc cccgacgacg tccggggtga cgcagcgacc
     3721 ctgcgggagg ccgtcaccac caccgggtgg ccgaagctcg gcgacgttcg aggccaggtg
     3781 atgctgttca tggacaacgg cggcgacctc cacgacctct acctcaccgg ccacccgtcg
     3841 ctcgagggtc gagccctgtt cacgagcgcc gccgaggacg gcgacgacga cgaggcggtc
     3901 gtgaaggtca acgacccggc ggaagcacca cgcatcaccc gcctggtgaa cgacggcttc
     3961 ttcgtccgca cccgcgccga cgccgacctc accgaggcgc agacgaacga caccaccgac
     4021 cgcgagatca cgctgcggtc cggcgcccag gtggtctcca ccgacttccc gatcgccgag
     4081 cccgctgcca acggctacgt cgtcgccttc ccggccgggc cacaggtccg atgcaacccg
     4141 gtcctcgtga ccgcgtgccc gacggactcc ctcgaacccg cgggctgacg ctcgctcaga
     4201 gcacgtacga gctggtgccc gtccgacgac gccaccccga cgccgcgaac gtccccccgt
     4261 cgaggtgcag cgtggtgccc gtcacgaacc gggccatgtc gccggcgagg aacacgatgg
     4321 cagcagcggc gtcctcgacc gaacccatgc gacccgtcgg ggtcgggtcg taggccgcgt
     4381 cgagcatcgc gcccgaggcc tcgacgagcc ccgcatcgcc gggcgtgggg atcatgtcgg
     4441 gggccacgca gttgacgcga accccctgcg gggccaattc gagcgccagg gtcttcgaca
     4501 ggttctcgac cgccgccttc atggccgaat agatcccgaa gccggggccg gcacgatggg
     4561 cctcgatcga cgtgacgttg acgaccgacc cgccgttcgt catgtgcgga agcgacgtac
     4621 ggatgcaact ggtcacctgg gtgaagttct cggcaatgag tgccgcctcg cccttggcgt
     4681 tgatgtcggc gaacgacgcc cagaagccac caccggcgtt gttgacgagg atgtcgatcc
     4741 ggccgtgcat cgccacggtc gcagcgacga gctcgtcgac cacgtcctga cgtcgaacgt
     4801 ccccgacgac ggagacgcac gagcggccca gcgcctccac ctccgccacg gtgcccgcca
     4861 gcccgtcggc ctcacgatcg accacggcga cgtgcgcccc gaatcgggca agcgcgatcg
     4921 ccgtcgctgc cccgatgccc tgcgccgcac cggtcaccac ggcgacccgg ccggtcagca
     4981 tcaccacgtc gggatcgacg cgggccggaa caccttcgct cggggccgtc tccatgtggg
     5041 cattcttgcg cgtcggtgga cgtaccctgt cgcccatgcg cgaatccgct gacccgcggc
     5101 tcggagagat cgagtccctc ctgatcgagc cgcagcgcga tccccacgtc gacatggtcc
     5161 tgagctcgcc cggccccgac cggtaccgcg tcgcgtcacc gctcggttcg gtcgagttcg
     5221 tccgactcat cgacacggga accgacgacg acggccgacc cgtcgaccgg taccgctacg
     5281 aggtcgatgc cgtggtcgat gccgacccca tcgcggacac gaccgacgac cggttcgtgg
     5341 gcctggcgct cgagcgcgag ggagtgttcc cgacgcggga cgtcaacgcg tatccccacg
     5401 cccacgacca gatcgcccag ttcttcgatt cgccacacgc gcccgacctc gtcgtccagc
     5461 acaccgccgg ccaccactac gagacgaacc tcggcgaaca cggctcgctc ggggtcgtac
     5521 aggcccgcgc gccgttcatc gcatcgggag ccggggtgcc ggcgcgcggg accctcgcgc
     5581 aatcggcgcg catggtggac gttgccccga ccgtgctcca cctgctcggt ggtgcgatcc
     5641 accccgaggg cgtcgggccg accgggtcgc ggaggtcagg ggcgctcctc gcccgtcagg
     5701 acggcgatgc cctcacgtcg atcgtcgacc ccgccg
//
LOCUS       DAOSOA010000214         5734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14182_length_5734_cov_5.107061, whole genome shotgun sequence.
ACCESSION   DAOSOA010000214 DAOSOA010000000
VERSION     DAOSOA010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14182_length_5734_cov_5.107061"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKY13_12205"
     CDS             <1..404
                     /locus_tag="PKY13_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT80746.1"
                     /translation="EARHVAFGVLSLKEYYEGLSDAEMFERQQFAFEAAVRMRDRFLQ
                     QEVFDRMGVPVKDAITAVMHTPTRQMFQMMLFSKIVPNCKKLGLLDRNDQWLRRRFEE
                     LGVIQFEDWTDTTDEMEAFALSNEELKIATA"
     gene            531..2237
                     /locus_tag="PKY13_12210"
     CDS             531..2237
                     /locus_tag="PKY13_12210"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HOT80747.1"
                     /translation="MTSTITPPAPEAPPARLDNGSPSRRAVLAATGVALAAAACAPAN
                     GGGAPTTTTTPTPPGPDPTSPPPPAPTTLPPTVPPTGGEGVTARIAIDKLTFGPRPGL
                     AAAIEARGVWGWIDDQLDPVGRNVAHAEQRVSGYTTLTNTHRQNYDVQDSDGGWDRII
                     EELDHATIQRAVYSDRQLYEVMCDFWTNHFNTWRQKTWMTFLKNRSHETVVRPNALGK
                     FSTMLLASAQDTAMLDYLDNLPSDASQPGGVNENYARELMELHTLGMVGGVQPFTEAD
                     VRGVAKIISGWSINWDDNANRYNFRFNPWQHSRDAVTVLGGAFTRPARTYGQGYDDGV
                     RLVNLLAHHPTTARHIAWKLCRRFVSDTPSDALVTSAAAVFTANDTAIAPTLRHIFAS
                     PEFVAGHKKKVRRPFEHLVGALRALDATMPTAAQTQTTDKLRATLENLGQPIFERHSP
                     DGYPDVNTFWVSSEGFLQRWAMAGRLARNRLTDPGQPDAIVVNLPALLPSPAPATVAD
                     LIRWVATNLANSPINDTDVADLCTACSFSPTAASTTVSGDATKLALAVGLVLCHPTFQ
                     QR"
     gene            2234..3559
                     /locus_tag="PKY13_12215"
     CDS             2234..3559
                     /locus_tag="PKY13_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOT80748.1"
                     /translation="MSDTMTNPAVPSLMSTLSRRGFLAGSGALVASATFGTKLAFASP
                     ESPASGDVIVLVFLRGGMDGLSMVAPFQMPSYRTLRPTIRVKDASEVADPANAGLPLV
                     AGGAVDPFALSGVFALHPGMAALHSTAWADGKLAVVHAAGMPATESSTRSHFDSMRNW
                     EVGSAALGQTTGFLNRYLNDVGADRIAGISGGSALARSLAGPVPAYSMGSIAGFGVSG
                     FSNNTKATNAITRWYDSGTGDVVAQVGADTMGAVSTVRSINWSDPRFAVQNGATYPGS
                     WFSSALREVAQLIRANIGLRAVCLDVGGWDTHSDMGAPEDPNGYFRGRCRELADGLAA
                     FYRDLGTQMDEVTVATISEFGRTIDENGTGGCDHGRGSAMLVMGGGIKGGVHGPFVPS
                     ITNGPEDDLTVLNDYRTVMAEVLTVRGGATHPGTLFPTWTPAAPLGVCR"
     gene            3569..4213
                     /locus_tag="PKY13_12220"
     CDS             3569..4213
                     /locus_tag="PKY13_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80749.1"
                     /translation="MHDQDLLRVLHAVRLAGINGADAVVERSEVGASRVASVLTEAQA
                     SGWVEQRVVVRDRRPSGWMLTAQGRRHGEALLAAEVALAGAAEALVAAYERFRTVNQQ
                     FLDVCTDWQLFPDEDGRRAVNDHSDPDYDRAVVERLVRLDEVIQPVLADLASALARFE
                     GYGERFAAARRLVEAGERDWFTRPLIDSYHTIWFELHEDLLATLGLERSHEHRS"
     gene            4295..5185
                     /gene="dapA"
                     /locus_tag="PKY13_12225"
     CDS             4295..5185
                     /gene="dapA"
                     /locus_tag="PKY13_12225"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT80750.1"
                     /translation="MSLDAPRFGRVITAMVTPFDEGGALDLDSAANLAAWLVDNGSDG
                     LVLAGTTGESPVLSDSEEVALTRAVRSAVKVPLLLGTGSNDTAYAVSATRRAEQLGVD
                     GVLVVGPYYNRPSQAGLDHYFRSVAAATPLPVVIYDIPVRTGRKINTATMLRLVHEVP
                     NLVGLKDAAGNPAETARFLRDAPDGFEVYSGDDPFTLPLLSVGAVGVISVASHWAGRQ
                     IGDMIAAFAKGDIGEAVRLNQGLIESYEFETGDEAPNPVPTKAMLRVLGLKVGQCRPP
                     MGVAPDWLEPRAREVLAGLT"
     gene            5246..>5734
                     /locus_tag="PKY13_12230"
     CDS             5246..>5734
                     /locus_tag="PKY13_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOT80751.1"
                     /translation="MAAEAVRITFLGGLGEIGRNCAAIEIEGRIMLLDCGLMFPDTDM
                     LGIDVVLPDFTWLRERADDIEGCILTHGHEDHVGALSYALQEFKFPIISSEFTLGLAR
                     GRIEENGVLKNTKLIVVKDNERRQFGPFDCEFLPITHSVPHGFATAFHTPQGTILHSG
                     DFK"
BASE COUNT          922 a         2088 c         1852 g          872 t
ORIGIN      
        1 acgaggcccg ccacgtcgcc ttcggtgtgc tctcgctgaa ggagtactac gaggggctca
       61 gcgacgccga gatgttcgag cgccagcagt tcgcgttcga ggccgccgtc cgcatgcgcg
      121 accgcttcct tcagcaggag gtcttcgacc gcatgggtgt tccggtcaag gatgcgatca
      181 cggccgtgat gcacacgccg acccgccaga tgttccagat gatgctgttc agcaagatcg
      241 tgcccaactg caagaagctc ggtctgctcg accgcaacga ccagtggctg cgccgccggt
      301 tcgaggagct cggcgtcatc cagttcgagg actggaccga caccaccgac gagatggaag
      361 cgttcgcgct ctccaacgag gagctcaaga tcgccacggc ctgaccggtt tgtccggcca
      421 gccggttttg tcccgcgaag gcgtgcggac cgcacgcctt cgcgggacaa aacgagccgg
      481 gtaggccatt cggctcaagt tcttgggtgc tccgcaccga tagaccgggc gtgacctcga
      541 ccatcacccc gcccgcaccg gaagcaccac cagcacggct cgacaacggc tcgccctcgc
      601 ggcgggccgt tctcgcggcc accggcgtcg cgctcgccgc agcggcgtgc gcgccggcga
      661 acggtggcgg cgcgccgacc accacaacga caccgacccc gccgggaccg gaccccacca
      721 gtccgccacc gcccgccccg acgaccctgc ctccgaccgt gccgccgacc ggtggcgagg
      781 gtgtcacggc gaggatcgcc atcgacaagc tcacgttcgg gccgcggccc ggtctcgcgg
      841 cggcgatcga ggcccgcggc gtctggggct ggatcgacga ccagctcgat cccgtcgggc
      901 gcaacgtcgc gcacgccgag cagcgggtgt ccggctacac cacgctcacc aacacccacc
      961 gccagaacta cgacgtccag gactccgacg gcggatggga tcggatcatc gaggaactgg
     1021 accacgcgac gatccagcgc gccgtctact ccgaccgcca gttgtacgaa gtcatgtgcg
     1081 acttctggac gaaccacttc aacacgtggc gccagaagac gtggatgacg ttcctcaaga
     1141 accgctccca cgagacggtg gtgcgaccca acgcactcgg gaagttctcg acgatgctcc
     1201 tcgccagcgc gcaggacacg gcgatgctcg actacctcga caacctcccg agcgacgcct
     1261 cccagcccgg gggagtcaac gagaactacg cccgcgaact catggaactc cacaccctcg
     1321 gcatggtcgg cggggtccaa cccttcaccg aggccgatgt gcgcggcgtc gccaagatca
     1381 tcagcggctg gtcgatcaac tgggacgaca acgcgaaccg ctacaacttc cggttcaacc
     1441 cctggcagca cagccgcgac gccgtcaccg tcctgggcgg agcgttcacc cgcccggctc
     1501 ggacctatgg ccaggggtac gacgacgggg tccgcctcgt caacctcctc gcccatcatc
     1561 cgaccacggc gcgccacatc gcctggaagc tctgccggcg attcgtgagc gacactccga
     1621 gcgacgccct cgtcacgtcg gccgccgcgg tgttcacggc caacgacacg gcgatcgccc
     1681 cgacgctgcg ccacatcttc gcgagccccg agttcgttgc cgggcacaag aagaaggtcc
     1741 gccgtccgtt cgaacacctc gtcggcgccc tgcgggcact ggatgccacc atgccgaccg
     1801 cggcgcagac ccagacgacc gacaagctgc gagcgaccct cgagaacctc gggcaaccca
     1861 tcttcgagcg acacagcccc gacggctatc ccgacgtcaa cacgttctgg gtgtcgtcgg
     1921 aggggttcct gcagcgctgg gcgatggcgg ggcggctcgc ccgaaatcgt ctcaccgacc
     1981 cgggccagcc cgacgcgatc gtcgtgaacc tgccggcact cctgccgtct ccggcgccgg
     2041 cgaccgtcgc cgatctgatc cggtgggtgg ccacgaacct cgccaactcg ccgatcaacg
     2101 acaccgatgt ggccgacctg tgcacggcct gttcgttcag ccccactgcg gcgagcacga
     2161 ccgtcagcgg cgacgccacc aaactcgccc tcgctgtcgg cctcgtcctg tgccacccga
     2221 ccttccaaca gcgatgagcg acacgatgac gaacccggca gtgccctccc tcatgtccac
     2281 cctgagccgt cgtggcttcc tcgccgggag tggtgcgctc gtggcgagcg ccacgttcgg
     2341 caccaagctc gcgttcgcct caccggagtc gccggcgagc ggcgacgtca tcgtcctcgt
     2401 cttcctgcgt ggcggcatgg acggcttgag catggttgca ccgttccaga tgccgtccta
     2461 ccggacgttg cggcccacga tccgggtcaa ggacgcatcg gaggtggccg atccggccaa
     2521 cgccggtctc ccgctcgtcg ccgggggagc ggtcgatccg ttcgcactgt caggggtgtt
     2581 cgccctgcac ccgggtatgg cggcgttgca ttcgacggcg tgggccgacg gcaagctcgc
     2641 cgtggtccac gccgccggaa tgcccgccac cgagagctcg actcgcagcc acttcgactc
     2701 gatgcgcaac tgggaggtgg gctcggcggc actcggccag accaccgggt tcctcaaccg
     2761 ctacctcaac gacgtcggtg ccgaccggat cgccggcatc tccgggggct cggccctggc
     2821 ccgcagcctc gccggaccgg tccccgccta ttcgatggga agcatcgccg ggttcggggt
     2881 cagcgggttc tccaacaaca ccaaggcgac gaacgccatc acccgctggt acgactccgg
     2941 caccggtgac gtcgtggcgc aggtcggtgc cgacacgatg ggcgccgtga gcactgtccg
     3001 ctcgatcaac tggtccgacc cccggttcgc cgtgcagaac ggcgccacct atccggggtc
     3061 gtggttctcg tccgccctgc gggaggtcgc ccagctgatc cgggcgaaca tcggtctccg
     3121 ggcggtgtgc ctcgacgtcg gcggctggga cacccacagc gacatgggtg cccccgagga
     3181 tccgaacggg tacttccggg gccggtgccg ggagctggcc gacgggctgg ccgcgttcta
     3241 tcgcgatctc gggacccaga tggacgaggt gacggtcgcg acgatctcgg agttcggtcg
     3301 cacgatcgac gagaacggca cgggcggctg cgaccacggg cgcggttcgg cgatgctcgt
     3361 catgggcggt gggatcaagg gcggtgtcca cggccccttc gtcccgagca tcaccaacgg
     3421 tcccgaggac gatctcacgg tcctcaacga ctaccggacg gtgatggccg aagtcctcac
     3481 ggtccgcggc ggggcgaccc atcccggcac cctgttcccg acgtggacgc ccgctgcgcc
     3541 gctgggggtc tgccggtagt ctcggcccat gcacgaccag gacctgttgc gggtgctgca
     3601 tgccgtgcgt ctcgccggca tcaacggggc cgacgccgtg gtcgaacgaa gtgaggtcgg
     3661 tgcgtcccgg gtggcctccg tcctcaccga ggcgcaggcg tcggggtggg tcgagcagcg
     3721 cgtcgtcgtc cgggaccgtc ggccgagcgg gtggatgctg acggcgcagg ggcgccggca
     3781 cggcgaggcg ctcctcgccg ccgaggttgc actcgccgga gcggccgagg cgctggtcgc
     3841 tgcctatgag cgcttccgaa cggtcaacca gcagttcctg gacgtgtgta ccgactggca
     3901 gctgttcccc gacgaggacg gacgccgtgc ggtcaacgat cacagcgatc ccgactacga
     3961 ccgggccgtc gtcgaacgac tcgtccgcct cgacgaggtg atccaacccg tacttgccga
     4021 tctcgcgtcg gccctcgccc ggttcgaggg ctacggcgaa cgcttcgccg cggcccgccg
     4081 cctcgtcgag gcaggcgagc gcgactggtt cacccgtccc ctcatcgact cgtaccacac
     4141 gatctggttc gaactgcacg aggacctgct cgcgacgctg ggcctggagc ggagccacga
     4201 acaccgatcg tgaccgctcg gtcaccccgc cagcggtgcg ccaccaaccg aaagcgcttc
     4261 ttgcggccat gctcatggca gtagcgtctc cgccatgagt ctcgacgctc cgcgcttcgg
     4321 ccgggtcatc accgccatgg tcaccccgtt cgacgagggg ggtgctctcg acctcgattc
     4381 ggccgccaac ctggcggcct ggctggtcga caacggcagc gacggcctgg tgctcgccgg
     4441 caccacgggt gagagcccgg tgctgagcga ctccgaggag gtcgcgctga cccgggcggt
     4501 gcgctccgcg gtcaaggtcc cgttgctgct cggaaccggc tccaacgaca ccgcgtacgc
     4561 ggtctcggcc acccgccgcg ccgaacagct cggcgtcgac ggtgtgctcg tcgtcggtcc
     4621 ctactacaac cgcccgtcgc aggccggcct cgaccactac ttccgctcgg tcgccgcggc
     4681 gaccccgctg ccggtcgtca tctacgacat ccccgttcgt accggccgca agatcaacac
     4741 cgccacgatg ctgcggctcg tgcacgaggt gccgaacctc gtcggcctca aggacgccgc
     4801 cggcaacccc gccgagaccg cccggttcct ccgcgatgct cccgacgggt tcgaggtgta
     4861 ctcgggcgac gaccccttca cgctcccgtt gctgtcggtc ggcgctgtcg gcgtgatcag
     4921 cgtcgcgagc cactgggccg gtcgtcagat cggcgacatg atcgccgcgt tcgcgaaggg
     4981 tgacatcggc gaggcggttc gcctcaacca ggggctgatc gagagctacg agttcgagac
     5041 cggtgacgag gctccgaacc cggtccccac caaggcgatg ctgcgggtgc tggggctcaa
     5101 ggtcggacaa tgccgccctc cgatgggagt tgcgccagac tggctcgaac cacgggcccg
     5161 cgaagtactc gccggcctga cctgaccggc gttcgcggcg tcaccgccag cgaccctccc
     5221 gtaaccaaag gactccacca cattcatggc tgccgaagcg gttcgcatca cgttcctggg
     5281 cgggctcgga gagatcggtc gcaactgcgc ggccatcgag atcgaggggc gcatcatgct
     5341 cctcgactgc gggctcatgt tccccgacac cgacatgttg gggatcgacg tcgtccttcc
     5401 cgacttcacg tggctgcgcg agcgggccga tgacatcgag gggtgcatcc tcacccacgg
     5461 tcacgaagac cacgtcgggg cgttgtccta cgcactgcag gagttcaagt tcccgatcat
     5521 cagctcggag ttcaccctcg gcctggccag gggccgcatc gaggagaacg gtgtcctcaa
     5581 gaacaccaag ctcatcgtcg tgaaggacaa cgagcgacgg cagttcggcc ccttcgactg
     5641 cgagttcctg ccgatcacgc actcggtgcc ccacggcttc gccacggcgt tccacacccc
     5701 gcagggcacg atcctgcact cgggcgactt caag
//
LOCUS       DAOSOA010000215         5728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14207_length_5728_cov_6.651860, whole genome shotgun sequence.
ACCESSION   DAOSOA010000215 DAOSOA010000000
VERSION     DAOSOA010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14207_length_5728_cov_6.651860"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..864)
                     /locus_tag="PKY13_12235"
     CDS             complement(16..864)
                     /locus_tag="PKY13_12235"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT80752.1"
                     /translation="MPLPHLPASAHRLVAVVATLTILSLTACGSDADSVADVTPTTAA
                     PGASTTAATTTSTTEPDPAYRSWIANVRSEVGSIVIHDSPGGPQLQLDTAGDGAVKPV
                     AISNPLPSGAPATFLIAQRAVEATGTLWHEVYLPVRPNGSTGWVADADVVLTNTDMAA
                     TIDLATHQLTLSEAGAVIATYPVAAGEAATPTPTGRFYVKELVEPPKANGTYGPLAYG
                     LSAFSPTLVDTEAFADGVIGIHGTNRPELIGSDVSHGCVRLRNEDVLDLERRQVPLGM
                     PVTITA"
     gene            complement(935..2416)
                     /locus_tag="PKY13_12240"
     CDS             complement(935..2416)
                     /locus_tag="PKY13_12240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT80753.1"
                     /translation="MPILRTVITDPAELERRSAPRDDLMVREFEVEPGVLAADDGPFS
                     RWDRRFTTTDGVTVETIDYQLAVPHWGWLVGLLLRRPVRTGLAPDQKPAWCPSDRLGR
                     REANILGLCATLAVVAGFLGGLIGQTITYIAADLGGTTATQANSLTVIRIGAVLTFAA
                     TALADQRGRRPLLRACLVIAGLACLVTAIAPDFATVTGSQLVARGLVAGAAYLIPIIC
                     AEELPAGSRAYAIGLMALPGGLGVGIVLWFVPFLDAADWAWRLLFVLALPALIVAILT
                     ARRLPETARFEHDDHVPAEHAHQHMRPSRLVVLAAGMFFLNIFTAPTQQLQTDYLRNT
                     RGLSSTAVAIFILATNTWGFIGIFAGAKLADRSSRRRAATIGLLGLAIGNTAMFCIGG
                     PPMWVASLVGSMVGAAVVPSLGALLPELFPTLRRGAANGLLNGAAVIGSMTGLYIVGQ
                     VVTDGIYGPTVAALALGPLIVAGLVWRLPETAGVELEALNPDD"
     gene            2462..3712
                     /gene="murA"
                     /locus_tag="PKY13_12245"
     CDS             2462..3712
                     /gene="murA"
                     /locus_tag="PKY13_12245"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT80754.1"
                     /translation="MDEMIVRPGGPLSGEIPISGAKNSVLKLMVATVLAPGRFVIRNV
                     PRISDVDWMAETLVALGLDVDWSEPQTLVIDSTGTITPEAPYRLVEQMRASTALLGAL
                     LARCGEARIAMPGGDDFGDRPIDLHLQGLQELGADIELSHGNIVATTSGLTGARVVLE
                     YPSVGATENLLLAATRATGITVIENAAREPEIVDLAAFVNRMGARVVGAGSSTITITG
                     VDELHSVEHEVIPDRVEAATYLTILGAARGEVLLRGARANDLQLLIGKLGEMGMRISP
                     DAAGIWAMASSRPTSVDLATLPYPGIATDYLPMLIALLSVGDGTSFATENLYQGRFRY
                     VGELTRMGANIRVESHHMAVRGVERLSGAPVRAFDIRAGAALVVGAFAAEGETVIHDA
                     HHLDRGYQDIVGRLRAVGADVSSR"
     gene            complement(3722..4687)
                     /gene="meaB"
                     /locus_tag="PKY13_12250"
     CDS             complement(3722..4687)
                     /gene="meaB"
                     /locus_tag="PKY13_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOT80755.1"
                     /translation="MAPVNPDDIEQLLDDAAGGRRAALARLLSVVERGGDGARVLGRL
                     AHPRGGQAYTVGITGAPGAGKSTLTNALCATVREAGDRIAVLAIDPSSPFSGGAILGD
                     RIRMGDHTLDDGVFIRSMATRGHLGGLSLATPSAVRVLDAVGFPWVVVETVGVGQVEV
                     EIVGSADTCVVVVNPGWGDAVQANKAGLMEIADVFVVNKADRAGAAETQNDLEGMLSL
                     KVADGWRPPVVPAVATSGEGVADVWTAVADHRRWLDESGEGARRRQGRVAAELRSIIA
                     VQLAARAAKLGGGSRFEELRDEVLARDIDPWSAVDEVLASIDSRS"
     gene            complement(4719..4982)
                     /locus_tag="PKY13_12255"
     CDS             complement(4719..4982)
                     /locus_tag="PKY13_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOT80756.1"
                     /translation="MRDKVAEVIEVVRPAIQADGGDITLLDVDEAAGIVTVELSGACV
                     SCPASTVTLKAGIERIMKDRVPGVTEVRQPGEDLVETGTTVSL"
     gene            complement(5159..>5728)
                     /locus_tag="PKY13_12260"
     CDS             complement(5159..>5728)
                     /locus_tag="PKY13_12260"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOT80757.1"
                     /translation="DLPAVDRWAIDGIPVTAVNRTLIDVGRYWTVGRVWSLLDHAVRD
                     ELTTYESFEARVHGLARQGRNGIGTAREVLLRLGLDGTWGFERAMRHALRDAGLPKPT
                     REFRVDIGPNRYYIDFAYPEANLGIECDSTQWHTLPSQVAAGLKRANEINGSGFLLLS
                     YSSSQLRDEREAVIAEISRHLRERSPGRM"
BASE COUNT          887 a         1978 c         2051 g          812 t
ORIGIN      
        1 ccgtgatgtc gcggctcacg ccgtgatggt gaccggcatg ccgagcggca cctgtcgacg
       61 ctccagatcg agcacgtcct cgttgcgcag acggacgcac ccgtggctca cgtcggagcc
      121 gatgagctcg ggtcgattcg tgccgtggat accgatgaca ccgtcggcga acgcctcggt
      181 gtcgaccagc gtgggggaga acgccgacaa gccgtaggcc aggggcccgt aggtaccgtt
      241 cgccttgggg ggttcgacga gttccttgac gtagaacctg cccgtcggcg tcggcgtcgc
      301 agcctcgccg gcggcgaccg ggtaggtggc gatgaccgcg cccgcttcgc tcagcgtgag
      361 ttggtgggtc gccaggtcga tcgtcgccgc catgtcggtg ttcgtgagca cgacgtcggc
      421 gtcggccacc cacccggtgc tgccgttggg gcgaacgggc aggtacacct cgtgccagag
      481 cgtcccggtc gcctcgaccg cccgctgcgc gatcaggaac gtcgccgggg cacccgacgg
      541 cagcgggttg gagatcgcca ccggtttcac cgcaccgtcg cctgccgtgt ccagctgcag
      601 ttgggggccg ccgggggagt cgtgaatgac gatcgagccg acctcggagc gaacgttggc
      661 gatccaactg cggtacgcgg gatcgggttc ggtcgtcgag gtcgtcgtgg ccgcggtggt
      721 cgacgctccc ggagcagcgg tggtcggggt gacgtcggca acagaatcgg cgtcggaacc
      781 gcacgccgtg agcgacagga tcgtgagcgt cgcaacgacg gcgaccaggc ggtgtgcgga
      841 ggcggggaga tgggggagcg gcacggggcc agcgtaacgg cgggcgcccc cgatcgatcg
      901 ccgccgaggc cggggccggc tcggtcgatc gggctcagtc gtcgggattg agggcctcga
      961 gctcgacgcc ggccgtttcg gggagccgcc acaccaggcc ggcgacgatc agcgggccga
     1021 gtgcgagcgc cgcgaccgtc gggccgtaga tgccgtcggt gacgacctgg ccgacgatgt
     1081 agagcccggt catgctgccg atgaccgcgg ccccgttgag gagtccgttc gcggcccctc
     1141 gtcgcaacgt cgggaagagc tcgggcagga gggcgcccag cgacggcacg accgccgcgc
     1201 cgaccatcga cccgaccagc gaggcgaccc acatcggtgg accgccgatg cagaacatcg
     1261 cggtgttgcc gatcgccagg ccgaggagac cgatcgtcgc ggcacgccgg cgactcgacc
     1321 ggtcggccag cttggccccg gcgaagatcc cgatgaagcc ccacgtgttc gtcgccagga
     1381 tgaagatggc caccgcggtc gagctgagcc ccctcgtgtt acgcaggtag tcggtctgca
     1441 gttgctgggt aggggccgtg aagatgttga ggaagaacat cccggcagcg agcaccacga
     1501 gccgcgacgg ccgcatgtgt tgatgggcgt gctcggccgg cacgtggtcg tcgtgctcga
     1561 agcgcgctgt ctccggcagt cgtcgggccg tgaggatcgc cacgatcaac gccgggaggg
     1621 ccaggacgaa gagcaggcgc cacgcccagt cggctgcatc gaggaacggg acgaaccaca
     1681 acacgatgcc gacaccgagc ccaccgggga gggccatcag gccgatggcg tacgcacggg
     1741 aaccggcggg caactcctcg gcgcagatga tcgggatgag gtacgcggcg ccggccacga
     1801 gaccacgggc gacgagctgc gagcccgtga ccgtcgcgaa gtcgggtgcg atcgccgtga
     1861 ccaggcacgc caagccggcg atcacgagac aggcccggag cagcgggcgt cggccccgct
     1921 ggtcggcgag cgccgtcgcg gcgaacgtca gcacggcgcc gatccggatg acggtcagcg
     1981 aattggcctg ggttgcggtg gtgcccccga ggtcggcggc gatataggtg atggtctgac
     2041 cgatgagccc accgaggaac cccgccacga cggcaagcgt cgcgcacagg cccaagatgt
     2101 tcgcctcccg ccggcccagg cggtcgctcg ggcaccaggc cggcttctgg tcgggcgcca
     2161 gaccggtgcg gacgggccgc cggagcagga gcccgacgag ccaaccccaa tgcggcacgg
     2221 cgagctggta gtcgatcgtc tcgaccgtca ccccgtcggt ggtcgtgaac cggcggtccc
     2281 atcgcgagaa cgggccgtcg tcggccgcga gcacccccgg ctcgacctcg aactcgcgca
     2341 ccatgaggtc gtcacggggg gcggatcgcc gttcgagctc ggcgggatcg gtgatgaccg
     2401 tacggaggat gggcatgggc ggaggtcacc gggcgtcggc gggccgagca tactggcggc
     2461 catggatgag atgatcgtcc gccccggcgg cccactgagt ggcgagatcc cgatctcggg
     2521 agcgaagaac tcggtgctca agctgatggt ggcgaccgtg ctcgctcccg ggcgcttcgt
     2581 catccgcaac gtgccccgga tcagcgacgt cgactggatg gccgagacgc tcgtcgccct
     2641 gggcctcgac gtggactggt ccgaaccgca gacgctcgtc atcgactcca ccggcacgat
     2701 cacacccgag gcgccgtatc gcctcgtcga gcagatgcgg gcgtcgaccg cgttgctcgg
     2761 agcgctgctc gctcgatgtg gtgaggcgcg catcgccatg ccgggcggag acgacttcgg
     2821 ggaccgtccg atcgacctgc acctgcaggg cctccaggaa ctcggcgccg acatcgagct
     2881 cagccacggc aacatcgtgg ccacgacatc ggggctgacg ggcgcccgcg tcgtcctcga
     2941 gtacccgagc gtcggcgcca ccgagaacct cctgttggcg gcgacacggg cgaccgggat
     3001 caccgtcatc gagaacgccg cccgcgagcc cgagatcgtc gacctcgcgg cgttcgtcaa
     3061 ccgtatgggc gcccgggtcg tgggcgcggg aagttccacg atcacgatca ccggcgtcga
     3121 cgagctgcac tccgtcgagc acgaggtgat ccccgaccgg gtcgaggccg ccacgtacct
     3181 cacgatcctc ggcgccgccc ggggagaggt gctcctgcgc ggcgcccggg ccaacgatct
     3241 gcagctgctc atcggcaagc tcggcgagat gggcatgcgg atctcgcccg acgccgccgg
     3301 tatctgggcg atggcgagct cccggccgac gtcggtcgac cttgcgacgc tgccctatcc
     3361 cggcattgcc accgactacc tcccgatgtt gatcgcactg ctgtcggtcg gcgacggcac
     3421 cagcttcgcg accgagaacc tgtaccaggg tcggttccgc tacgtcgggg agctcacccg
     3481 gatgggcgcc aacatccgcg tcgagagcca ccacatggct gtccgtgggg tggaacgcct
     3541 gtcgggcgcg ccggttcgtg ccttcgacat ccgcgccggc gccgcgctcg tcgtcggggc
     3601 cttcgccgcc gagggcgaga ccgtcatcca cgatgcccat cacctcgacc gcggttacca
     3661 ggacatcgtc ggccgcctcc gggccgtcgg cgccgacgtc agctcccggt agacgatccg
     3721 gtcacgaccg ggagtcgatg ctcgccagga cctcgtcgac agcggaccag gggtcgatgt
     3781 cgcgggcgag tacctcgtca cggagttcct cgaatcggga gccgccaccg agcttcgcgg
     3841 cgcgggctgc gagctgcacg gcgatgatcg accgcaactc cgcggcgaca cgaccctgcc
     3901 gacggcgcgc cccctcgccg ctctcgtcga gccagcggcg gtgatcggcg accgcggtcc
     3961 acacgtcggc caccccctca cccgacgtcg cgaccgccgg gacgaccggc ggccgccagc
     4021 cgtccgcgac cttcaggctg agcatgccct cgaggtcgtt ctgggtctcg gcggcgccgg
     4081 cgcggtcggc cttgttgacc acgaagacat cggcgatctc catcaggccg gccttgttgg
     4141 cctggacagc gtcgccccag ccggggttga cgaccacgac gcacgtgtcg gccgagccga
     4201 cgatctcgac ctcgacctgg cccaccccga ccgtctcgac gacgacccac gggaacccaa
     4261 cggcgtcgag cacccgcacc gccgacggcg tggccaggga caacccaccg agatggccac
     4321 gcgtggccat ggagcggatg aacacgccgt cgtcgagggt gtggtcgccc atacggatgc
     4381 gatcgccgag gatcgctccg ccggagaacg gcgacgacgg gtcgatggcg agcactgcga
     4441 tccggtcacc ggcctcgcga accgtcgcgc agagggcgtt ggtgagtgtc gacttcccgg
     4501 cgccgggggc accggtgatg ccgaccgtgt aggcctgccc gccgcgggga tgggccaggc
     4561 ggccgaggac ccgggctccg tcgccccctc gctcgacgac ggacaggagg cgcgccagcg
     4621 cggcgcggcg gccaccagca gcgtcgtcga ggagctgctc gatgtcgtcg ggattcacgg
     4681 gggccatgac cccgacgagg gtagggcgat gccgacgctc agagcgagac ggtggtgccg
     4741 gtctcgacga ggtcttcccc cggttgccgg acctcggtca cgccggggac ccggtccttc
     4801 atgatgcgct cgatgccggc cttgagcgtg accgtcgagg cgggacagct cacacaggcg
     4861 ccgctcagct cgaccgtgac gatgccagcg gcctcgtcga catcgagcag ggtgatgtcg
     4921 ccaccgtcag cctggatggc cgggcggacg acttcgatga cttcggcgac cttgtcgcgc
     4981 acgacgaact cctctgacga accggtgctg ggtccacgtc ccaggctaga gcccggcact
     5041 ccgcgaggta caacgccacg aaggcgcccg cttgttccca gcgcacctcg ccggtgcccc
     5101 acctccgagg tttcggtcgc gcaatcgccc gctcagcggg catctgcgcg cccgaatctc
     5161 acatgcgccc gggtgagcgc tcgcggagat gcctcgaaat ctcggcgatg accgcctccc
     5221 tctcgtcccg cagctgcgac gaggagtaac tcaggaggag gaacccactg ccgttgatct
     5281 cattcgcccg cttcaggcct gcggcgacct gggacggcaa cgtgtgccat tgcgtcgagt
     5341 cgcattcgat gccgaggttg gcctcgggat aggcgaagtc gatgtagtac cggttggggc
     5401 cgatgtcgac gcggaactct cgggtcggct tcgggagccc ggcatctcgc agcgcgtgtc
     5461 tcatggctcg ttcgaatccc cacgtgccgt cgagcccgag ccggaggagc acttctcgag
     5521 cggtgccgat gccgttgcgg ccctgtcggg cgagcccatg cacccgggct tcgaacgact
     5581 cgtacgtcgt gagctcgtcg cggacggcgt ggtcgagcaa cgaccagacg cgtccgacgg
     5641 tccagtagcg gccgacgtcg atgagcgtcc ggttcacggc tgtgaccgga atgccgtcga
     5701 tcgcccagcg atccaccgcc ggcaggtc
//
LOCUS       DAOSOA010000216         5706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14286_length_5706_cov_6.991860, whole genome shotgun sequence.
ACCESSION   DAOSOA010000216 DAOSOA010000000
VERSION     DAOSOA010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14286_length_5706_cov_6.991860"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..920)
                     /gene="mutM"
                     /locus_tag="PKY13_12265"
     CDS             complement(108..920)
                     /gene="mutM"
                     /locus_tag="PKY13_12265"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOT80758.1"
                     /translation="MPELPEVDTIRSQLDPVLTGRQLVAGWSHPSAKFTDAVEAVGTT
                     IDAVGRRGKYLINPLDDGRELIVHLGMTGSLRVTAHPATDDDPYVRAWWDLDDGNRLV
                     FRDVRRFGRIAVLEAGRYDGMPTLASLGLDPFDPAFDGDALWSGLRSSGRHLKTQLLS
                     QRPVAGVGNIYADEAFWLAGVNPADRRITRPRAAALADAIRTALRSGLDHGGTTLRDY
                     VDADGRSGDNQHHLHCYGRGGEPCERCATPLRRRVLDARTTTWCPVCQPRRG"
     gene            977..1921
                     /locus_tag="PKY13_12270"
     CDS             977..1921
                     /locus_tag="PKY13_12270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT80759.1"
                     /translation="MSAGGSKKAIIAAFLANLGIAVGKFVGFLITGASSMLAESIHSL
                     ADTGNQALLLIGGRRSLRSADDEHQFGYGRERYFYAFVVALVLFTLGSVFAIYEGIHK
                     LEHPEAVESPIVAIAILGVALGLEGFSLRTAVKEADHLRNGEGWFDFIRHAKTPELPV
                     VLLEDTGAMIGLVVAFVALLLAWQVDPVFDGVGTLIIGGLLGVIAVVLAVEMKSLLIG
                     EAASPREQAAIRAAIDDDPRSRRLVHIRTEHLGPEELLVAAKVAYDPGLSIEGLAAAI
                     DETEIAIRAAVPTARLIYIEPGLVELKGAAGGSAEPAG"
     gene            2052..2681
                     /locus_tag="PKY13_12275"
     CDS             2052..2681
                     /locus_tag="PKY13_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT80760.1"
                     /translation="MGSDFVAIKDHGAAKQEPDLDTRERLVRAAIEVFLEKGYGGTRV
                     QDIARRAGFTSGALYVHFPSRTALLGEAIIREGQTIMTGLADQIDAAADTPDSIADLL
                     AGFSSGAPTKLDLLLLEALALASRSDEAKEVLATALDGFVEILMKRINVARERGDIDT
                     SIDTEALRQVFVAWIFGGVVVKALKIGTAPIDSLTDATRRLIGGLAPTA"
     gene            2749..3573
                     /locus_tag="PKY13_12280"
     CDS             2749..3573
                     /locus_tag="PKY13_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOT80761.1"
                     /translation="MTTLAVVNQKGGVGKTTVALGLAAVAARRDLSVVVVDLDPQANA
                     TSGMGVWSPVHTVAHGLAADTDGALVDVVAATGWGPDLFDVPPRLAPSSPDLAQLEPQ
                     MAVDPIGAQDRLRRALIGVDADLVIIDCPPSLGLLTINGLFAADHAVIVTEPSAWAVD
                     GVAQILRNISRIAERRNGTPTLSGVVVNRLARTRDARYWDEQLVETYGPDVIRPAIAL
                     KAAVAEASAQSLPITAVTRAGAAEAAAQFDAVLDRLVPAAPSHPEASRDGVADAAL"
     gene            3560..3865
                     /locus_tag="PKY13_12285"
     CDS             3560..3865
                     /locus_tag="PKY13_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80762.1"
                     /translation="MPPYDPKANRPKLVPVDDESAPVDALLGPADSSTSVAPTAPQRT
                     HLSVVSDDERAPRSVPDQPVAPTGSSRPDPKVLLAVAVTAIAVVVGSIVVARRRSSN"
     gene            3881..>4206
                     /locus_tag="PKY13_12290"
     CDS             3881..>4206
                     /locus_tag="PKY13_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT80763.1"
                     /translation="MHHFDETTERILQRATAYVRERLSFEPVPLDHRGDAATLDREAP
                     SLIGESGNDPDLVFDQYEHILSPAVISVDSPRFLSFIPAAPTKASMLFDMVVSASSLN
                     GTSWLES"
     assembly_gap    4207..4306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4307..4987
                     /locus_tag="PKY13_12295"
     CDS             <4307..4987
                     /locus_tag="PKY13_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOT80764.1"
                     /translation="AGTTNAGIIDDLAGIAAVAGDHHIWFHVDAAYGGAGLLSSTIRH
                     RYDGIERADSMIVDPHKWLFAPLDCAILLYRRPALAKGVHTQNASYLDSIHADEDEWN
                     PTDYAYHLTRRARGLAMWFSLATHGLGAYRRAVDRALEVTDYAARRIEAIDELELVRP
                     PDLSIVMFRRRGWTDEQYDAWSEQLLADGVGFVLPSRWEGETILRFAILHPDTTEAII
                     DEILVTLA"
     gene            5117..>5706
                     /locus_tag="PKY13_12300"
     CDS             5117..>5706
                     /locus_tag="PKY13_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80765.1"
                     /translation="MHPRRPTRSRTRPGRLHRRSRGSQRIRPTSRVIVISVLATSLGA
                     PTFGVGALALSMASASAATTRDVAVATEALASAKASRVAADRRHTELAAQLDELTARL
                     DAEFAGAAELAADLEAARSEMRRSAITAFISGGDGDRAFEFFTHGDLAELSGRRTFSQ
                     SRTLDWADAMTAYEALKRDNDPKLVALSEQREALAS"
BASE COUNT          848 a         1977 c         1880 g          901 t
ORIGIN      
        1 cggatcggac ggtggtgcgc ttgagctgtt ggatcatgga gccaccatcg acgagcgatg
       61 tcagggcccc gtgggtgagg tgtgagggaa ctgtgagcgg tccggcgtca gccgcgtcga
      121 ggttggcaga ccggacacca ggtggtcgtg cgtgcatcga gcacacgacg acggaggggg
      181 gtggcgcagc gttcgcaggg ttcgccgccg cgtccgtagc agtggaggtg gtgctggttg
      241 tcgccgctgc gtccgtcggc gtcgacgtag tcgcgaagcg tcgtgccgcc gtggtcgagg
      301 ccggagcgca acgcggtgcg gatcgcatcg gccagcgccg cagcgcgggg ccgggtgatg
      361 cgtcgatcgg ccgggttcac accggcgagc cagaacgcct cgtcggcgta gatgttgccg
      421 acgccggcca ccgggcgctg ggacaggagc tgggtcttga ggtgccgacc gctcgaccgg
      481 aggcccgacc acagcgcgtc gccgtcgaag gccggatcga acgggtcgag cccgagcgac
      541 gcgagtgtgg gcatgccgtc gtagcggccg gcttcgagca cggcgatccg tccgaaacgc
      601 cgtacgtcgc gaaagacgag gcggttgccg tcgtcgaggt cccaccatgc ccgcacgtac
      661 gggtcgtcgt cggttgcggg gtgggcggtg acccggagcg agccggtcat gccgaggtgg
      721 acgatgagtt cgcggccgtc gtcgagagga ttgatgaggt acttgccccg acggcccacg
      781 gcatcgatcg tcgtcccgac ggcctcgacg gcatcggtga acttggccga cgggtgactc
      841 caaccggcga ccagctgccg cccggtcaac accgggtcga gctgggatcg gatcgtgtcc
      901 acctccggga gctcgggcat aggatccagc ctcgcagacg gcggagtgtc gctccgtccg
      961 acccgtgagg agcccgatgt cagccggtgg tagcaagaag gcgatcattg ccgcgttcct
     1021 cgcgaacctc gggatcgccg tcggcaagtt cgtgggcttc ctcatcacgg gcgcgtcctc
     1081 gatgctcgcg gagtccatcc actccctcgc cgacaccggc aaccaggcgc tgctcctgat
     1141 cggtgggcgg cggtcgctgc gatcggccga cgacgagcac cagttcgggt acggccgcga
     1201 gcggtacttc tatgccttcg tggtggcgtt ggtcctgttc acgctggggt cggtgttcgc
     1261 gatctacgag ggcatccaca agctcgagca ccccgaggcg gtcgagtcgc cgatcgtcgc
     1321 gattgcgatc ctgggggtgg cgctcgggct cgaggggttc tcgctccgga cggccgtgaa
     1381 ggaagcggac cacctccgca acggcgaggg gtggttcgac ttcatccgcc acgccaagac
     1441 gccggaactg cccgtcgtcc tgctcgagga caccggggcg atgatcggcc tcgtggtggc
     1501 gttcgtggcg ctcctcctgg cctggcaggt cgacccggtg ttcgacggcg tcggcacgtt
     1561 gatcatcggc ggtctcctcg gggtcatcgc cgtggtgttg gccgtcgaga tgaagagcct
     1621 gctcatcggc gaggcggcat cgcctcgcga gcaggcggcg atccgtgcgg cgatcgacga
     1681 cgacccgaga agtcggcgac tcgtgcacat ccgaaccgaa cacctcggtc cggaggagct
     1741 gctcgtcgcg gccaaggtgg cctacgaccc gggactgtcc atcgaggggc tcgccgcggc
     1801 gatcgacgag accgagatcg cgatccgggc cgcagtgccg acggcccgcc tgatctacat
     1861 cgaacccggc ctcgtcgagc tgaagggtgc agccggaggg tcagcggagc ccgctgggtg
     1921 actccactcc ttcaccgaac aggaaaatta cctaacggtg ctataatggg ctatctttgc
     1981 aattgaaact atgtgtggtt tgattgggga ccgcacacgg tgggtacttt tcaccgaacc
     2041 gaggaggacc agtgggatcg gattttgtcg caatcaagga tcatggtgct gcgaagcagg
     2101 aacccgatct cgacactcgt gaacggctcg tgcgcgccgc catcgaagtg ttcctcgaaa
     2161 agggctacgg cggtacccgt gtgcaggaca tcgcccgtcg ggccggcttc acgtcgggcg
     2221 cgctgtatgt ccacttcccg agccgcaccg ccttgctcgg cgaggcgatc atccgcgagg
     2281 gccagacgat catgaccggc ctcgccgatc agatcgacgc cgccgccgac acccccgact
     2341 cgatcgccga cctcctcgcc ggcttcagct cgggcgcgcc gaccaaactc gatctcctcc
     2401 tcctcgaggc gctggcgctc gcgtcgcgtt ccgacgaggc gaaggaagtc cttgcgacgg
     2461 cgctcgacgg cttcgtcgag atcctcatga agcgcatcaa cgtggcgcgt gagcgtggcg
     2521 acatcgacac ctccatcgac accgaggcgc tccggcaggt gttcgtggca tggatcttcg
     2581 gcggtgtggt cgtgaaggcc ctcaagatcg gcaccgcgcc gatcgactcg ttgaccgacg
     2641 ccacccgccg cctcatcggt ggcctcgcgc ccacggcctg acgctccaac gtttccgtca
     2701 cggcccaccc acccctttct gggcggcaac cgctagcgtg atcctccgat gacgacgctg
     2761 gcggtggtca accagaaggg cggggtgggc aagacgaccg tcgcactggg tctcgccgcc
     2821 gttgcggcgc gtcgcgacct ctccgtcgtc gtcgtcgatc tcgatccgca ggccaacgcg
     2881 acctccggca tgggggtctg gtcgcccgtc cacaccgtcg cccacggtct cgcggccgac
     2941 accgacggcg cgctcgtcga tgtcgtggcc gccacgggat ggggtcccga cctgttcgac
     3001 gtccccccac gactcgcacc cagctcgccc gacctcgcgc agctcgagcc ccagatggcc
     3061 gtcgacccca tcggcgcaca ggaccgcctc cgccgcgcgc tcatcggtgt ggatgccgat
     3121 ctcgtgatca tcgactgccc gccgtcgctc gggctcctca cgatcaacgg cctgttcgcc
     3181 gccgaccacg cggtcatcgt gaccgaaccg agcgcctggg ccgtcgacgg cgtcgcccag
     3241 atcctgcgca acatcagccg catcgccgag cgccgcaacg gcacgccgac actctcgggc
     3301 gtcgtcgtca accggctcgc ccgcacccgc gacgcccggt actgggacga gcagctggtc
     3361 gagacctatg gacccgacgt catccgcccg gcgatcgcgc tgaaggcggc ggtggcggaa
     3421 gcgtcggccc agtcgctgcc gatcaccgcg gtcacgcggg ccggtgccgc cgaggccgca
     3481 gcgcagttcg acgcggtgct cgaccggctg gttccggcag cgccgtcaca tcccgaagcc
     3541 tcgcgagacg gagtcgccga tgccgcccta tgacccgaag gccaatcgcc ccaagctggt
     3601 ccccgtcgac gacgagtcgg cgcccgtcga cgcgctgctc ggtcccgccg actcgtcgac
     3661 gtcggtcgct ccgaccgcgc cccaacgcac ccacctgtcc gtcgtgtccg acgacgaacg
     3721 ggcgcctcgc tccgtgcccg accagccggt ggctccgacg ggctcgtcgc gtcccgaccc
     3781 caaggtgctg ctcgccgtgg cggtcacggc catcgccgtc gtggtcggat cgatcgtcgt
     3841 cgcccgtcgc cgcagcagca actgacggct tgccgtcacc gtgcaccact tcgacgagac
     3901 caccgaacgc atcctccagc gggccacggc ctatgtccgt gagcgactga gcttcgagcc
     3961 ggttccgctc gaccatcgcg gcgacgccgc cacgctcgac cgcgaggccc cgtcgctcat
     4021 cggagagtcg ggcaacgacc ccgacctcgt gttcgaccag tacgagcaca tcctgtcgcc
     4081 cgcggtgatc tcggtcgaca gcccgcggtt cctgtcgttc atccccgcgg cgccgaccaa
     4141 ggcctcgatg ctgttcgaca tggtggtgtc ggcgtcgtcg ctcaacggca cctcctggct
     4201 cgagtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgg gaacgaccaa
     4321 tgccggcatc atcgacgatc tggccggcat cgccgccgtc gccggcgatc accacatctg
     4381 gttccacgtc gacgctgcgt acggcggggc ggggctcctg tcgtccacga tccgtcatcg
     4441 ctacgacggc atcgagcgag ccgactcgat gatcgtcgac ccgcacaagt ggctgttcgc
     4501 cccgctcgac tgcgccatcc tcctctaccg gcggccggcc ctcgccaaag gggtgcacac
     4561 gcagaacgcg tcctacctcg actcgatcca cgccgacgag gacgagtgga acccgacgga
     4621 ctacgcctac cacctcaccc gccgggcccg gggcctggcc atgtggttca gtctcgcgac
     4681 ccacgggctc ggcgcgtacc ggcgcgccgt cgatcgcgcc ctcgaggtga ccgactatgc
     4741 ggcccgtcga atcgaggcga tcgacgaact cgaactggtg cgcccgcctg acctgtcgat
     4801 cgtcatgttc cgtcgtcgag gttggaccga cgagcagtac gacgcgtggt ccgagcagct
     4861 gttggcagac ggcgtcggat tcgtgttgcc gtcgcggtgg gagggggaga cgatcctgcg
     4921 gttcgcgatc ctgcaccccg acacgaccga ggcgatcatc gacgagatcc tcgtcacact
     4981 cgcctgacat ttcggcatag ctcgtacacc atcgacccct cgaagtgggg gatgcggttg
     5041 cttttgttgc agttccatga caaggatcgt gcccgttcgc acgaatcgac cggttcccgc
     5101 ggctcctctg ctgtccatgc acccacgtcg acccacccgc tcccgcaccc gtcccgggcg
     5161 gctccaccgt cggtcccgcg ggtcgcagcg catccgcccg acgtcgcgcg tgatcgtgat
     5221 ctcggtgctg gccacgtcgc tcggcgcccc gacgttcggg gtcggagcgt tggcactgtc
     5281 gatggcctcg gcatccgcgg cgacgactcg cgacgtcgcg gtcgcgaccg aggcactggc
     5341 gtcggccaag gcgtcccgag tcgcggcgga ccgccgtcac accgagctcg ccgcgcagct
     5401 cgacgaactg accgcccggc tcgacgccga gttcgccggt gcggccgaac tggccgctga
     5461 tctcgaggcg gcccggtcgg agatgcggcg gagcgcgatc acggcgttca tcagcggtgg
     5521 tgacggtgac cgggccttcg agttcttcac ccatggcgac ctcgccgaac tgtcgggtcg
     5581 ccggacgttc tcccagagcc gaaccctcga ctgggccgac gcgatgacgg cctacgaggc
     5641 actcaagcgc gacaacgacc cgaagctcgt tgcactgtcc gaacagcgag aggcgctggc
     5701 cagcaa
//
LOCUS       DAOSOA010000217         5646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14478_length_5646_cov_5.507065, whole genome shotgun sequence.
ACCESSION   DAOSOA010000217 DAOSOA010000000
VERSION     DAOSOA010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14478_length_5646_cov_5.507065"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1407)
                     /locus_tag="PKY13_12305"
     CDS             complement(169..1407)
                     /locus_tag="PKY13_12305"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOT80766.1"
                     /translation="MTESASRESSGIFATIPDAVAAIARGEIVVVVDDEDRENEGDLI
                     MAAEHATPEAITFFVRHTSGVICAPLTSDRLEELDIPLMVHDNTEAQRTAFMHTVDLR
                     HGTSTGISAADRSATIKALCDPATRPGDLARPGHIFPLRYAEGGVLKRAGHTEAAVDL
                     ARLAGCYPAGVLCEIVNDDGTMARVPDLVEFCKEHNLLMISIAQMVKYRRQTEKLVRR
                     VAEARIPTLWGDFTCYVYESLLDGEQHVAMVKGAVAGETDVMVRVHSECLTGDVFGSM
                     RCDCGTQLDGAMARIAEEGMGALVYLRGHEGRGIGIGHKIRAYNLQEQGMDTIEANVA
                     LGLPVDSREYGIGAQILNDLGITTMRLITNNPSKFGGLDGFGLEITGRVSIPTVPNAE
                     NIGYLRTKKEQMGHLIEGVD"
     gene            complement(1404..2030)
                     /locus_tag="PKY13_12310"
     CDS             complement(1404..2030)
                     /locus_tag="PKY13_12310"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOT80767.1"
                     /translation="MFTGIVEELGTVVSREGAKLRIAATTVLDGVQLGDSTAVNGCCL
                     TVVAWEPPVDGAAGWWEADVSDETYSRTSLGDLAPGDPVNLERPVRLMDRLGGHLVQG
                     HVDAVGTITAPAPDLAVRMDPALTRYVVEKGSITVDGVSLTVVDALDDGFTVALIPHT
                     ADVTTLGVRKVGDAVNLEVDVTAKYVERLLAAHLPDGKPAESPEGNQS"
     gene            complement(2030..3085)
                     /gene="ribD"
                     /locus_tag="PKY13_12315"
     CDS             complement(2030..3085)
                     /gene="ribD"
                     /locus_tag="PKY13_12315"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOT80768.1"
                     /translation="MSETFNALDRECMTRAIAAAAQVRCATSPNPWVGAVVRAADGSI
                     SEGATREVGREHAEIVALVAAGEAAQGATVYVTLEPCSHTGRTAPCADALIEAGVARV
                     VVALADPDERVNGNGIAKLRAAGITVDVGLYEDRVRQQLAPYIKHRTTGRPWVVLKLA
                     ASMDGATAAPDGSSQWITGAPARADGHRLRAESDAIVIGAGTVRRDDPSLTVRDYHPP
                     VLPESGSVDPIRVVLGTAPATAQVQPCREMKGQLPDILDELGADGVLQVLVEGGAQVA
                     GDFHRAGLVDRYVIYVAPALFGGGDARGLFTGFGAYSIDDVWRGRFETVERVGGDLRI
                     ELSPGAYDAVPVAGRNA"
     gene            complement(3202..3723)
                     /locus_tag="PKY13_12320"
     CDS             complement(3202..3723)
                     /locus_tag="PKY13_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOT80769.1"
                     /translation="MSPHDHESAQSPDGKPLKAKILTVSDGVMAGTREDKSGVAIGEH
                     LTAAGFRIVEHRVCADGSETVAHALAHMAYNFLGLIVSTGGTGFSPRDMTPEGTVRIL
                     DRRAYGLSEAMRSVNPLGRLSRGVAGTRGRALILNVPGSTSGSIEMLDAVLDVIPHAI
                     RLMGGANDEHPVG"
     gene            complement(3820..4980)
                     /locus_tag="PKY13_12325"
     CDS             complement(3820..4980)
                     /locus_tag="PKY13_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT80770.1"
                     /translation="MSAPVPATPVTSRSVALAALVEIEADGAYANLRLGPLLEKSGLD
                     DRDRRLVTELVYGTIRRRRSLDYLVDRFLVNDPPPAARAALRLGAYQLRFTDIPDHAA
                     VSATVDVAPKRFRGLINAVLRKVAKAPVNWPDDATRLSYPDWIVERFRADLGDEAAAD
                     ALETMNRAPEVNVRDDGYTQDRSSERVVAALGAAAGDLVVDVCAAPGGKATALAGAGA
                     HVVAADLRPKRVRLVAGNARRTAADRPDSGAVWPLVADARHAPLRAGVADIVLVDAPC
                     SGLGVLRRRPDARWRITANDVDELSVIQSAILDASAELVAPGGLLAYSVCTLTRSETI
                     DVAERFADTHPGFEAVAPPEDPWIPWGSGALLLPQADDSDGMALFTWRRPLS"
     gene            complement(4977..>5646)
                     /locus_tag="PKY13_12330"
     CDS             complement(4977..>5646)
                     /locus_tag="PKY13_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOT80771.1"
                     /translation="LGLRVEHDADAVLSSDASLGVVVAFGKILRPHLLAHVPMVNLHF
                     SLLPRWRGAAPVERAILAGDAETGVCVMAVEEGLDTGGVYARATTPVGTKRLTELWDE
                     LSVTGSQLLVDWLPTAFDGDRLIAPEPQVGEVTYAAKLTADDRRVDLTASAEQQLAVI
                     RLGNAWTMLGEGETAKRLKVLAGHLDADGSLRITEVQPEGKRPMAYEDWLRGTPVDLR
                     GGLS"
BASE COUNT          859 a         1902 c         2049 g          836 t
ORIGIN      
        1 tcgcgaccaa ggggagctcg aggctcccgg ggaccgtgat caagctgatc gcgtcctcgg
       61 cgatgcccgc cgccaccaga tgcgagtggc aggcagtcgt cgcgacattg acgagatcgc
      121 tgtgccactg cgccgcgatc atcgcgatgc ggacgtcggt gccggccatc agtcgacgcc
      181 ttcgatcagg tgacccatct gttccttctt ggtccggagg tagccgatgt tctcggcgtt
      241 ggggacggtc gggatggaga ctcggcccgt gatctcgagg ccgaacccgt cgaggccgcc
      301 gaacttggag gggttgttgg tgatgaggcg catcgtggtg atgccgaggt cgttgaggat
      361 ctgagcaccg atgccgtact cgcgggagtc gaccggcagg ccgagcgcca cgttggcctc
      421 gatggtgtcc atgccctgct cctgcaggtt gtacgcccgg atcttgtggc cgatgccgat
      481 gccacgaccc tcgtggcccc ggaggtacac gagggcgccc atgccctctt cggcgatgcg
      541 ggccatggcg ccgtcgagct gggtgccgca gtcgcagcgc atcgagccga acacgtcgcc
      601 ggtgaggcac tccgagtgca ctcggaccat cacgtcggtc tcgccggcga ccgcgccctt
      661 gaccatggcc acgtgctgct caccgtcgag cagcgattcg tacacgtaac aggtgaagtc
      721 gccccagagg gtggggatcc gtgcctcggc gacgcgccgc accagcttct cggtctgtcg
      781 tcggtacttg accatctggg cgatcgagat catcaggagg ttgtgctcct tgcagaactc
      841 gacgaggtcg ggaacacgcg ccatcgtgcc gtcgtcattc acgatctcgc agagcacacc
      901 cgcggggtaa cacccggcga ggcgggccag gtcgaccgcg gcctcggtgt ggccggcccg
      961 cttgagcacg ccgccctcgg cgtaacgcag cgggaagatg tggccggggc gggcgaggtc
     1021 gccgggacgc gtggccggat cgcacagggc cttgatggtc gccgaccggt cggcagccga
     1081 gatccccgtc gaggtgccgt gacggaggtc gacggtgtgc atgaacgccg tgcgctgcgc
     1141 ctcggtgttg tcgtgcacca tcagcgggat gtcgagttcc tcgagacgat cgctcgtgag
     1201 gggggcgcag atcacacccg acgtgtggcg gacgaagaac gtgatcgcct cgggcgtcgc
     1261 gtgctcggcc gccatgatga ggtcgccctc gttctcgcga tcctcgtcgt cgacgacgac
     1321 gacgatctca ccgcgagcga tggccgcgac ggcgtcgggg atcgtggcga agatgcccga
     1381 ggactctcgg gatgctgact cggtcatgac tggttccctt cagggctctc ggctggcttc
     1441 ccatcgggaa ggtgtgcagc gaggagccgc tcgacgtatt tggcggtcac atcgacctcg
     1501 aggttgacgg cgtcgccgac cttgcgcacc ccgagcgtcg tgacgtcggc cgtgtgcggg
     1561 atgagcgcga cggtgaagcc gtcgtcgagg gcatcgacca cggtcaggct gacaccgtcg
     1621 acggtgatcg aacccttctc gacgacgtag cgggtgagcg ccgggtccat gcgaaccgcg
     1681 agatcggggg cgggcgccgt gatggtgccg accgcgtcga cgtggccctg cacgaggtga
     1741 ccgccgaggc gatccatgag ccggaccgga cgctcgaggt tgaccggatc gccgggggcg
     1801 aggtcgccga ggctggtgcg ggagtaggtc tcgtcggaga cgtccgcctc ccaccagccc
     1861 gcagcgccgt cgacgggcgg ctcccaggcc accacggtca ggcaacagcc attgaccgcg
     1921 gtcgagtcgc cgagttgcac accgtcgagg acggtggtcg ccgcgatgcg caacttggca
     1981 ccctcacggg acacgaccgt gcccagttct tccacgatgc ccgtgaacat caggcattcc
     2041 ttcccgccac agggacggcg tcgtacgcgc ccgggctcag ctcgattcgc aggtcgccgc
     2101 cgacccgttc gacggtctcg aaacggcccc gccacacgtc gtcgatggag tacgccccga
     2161 acccggtgaa caggccacgg gcatcgccgc cgccgaacag cgcgggagcc acgtagatga
     2221 cgtaccggtc gaccagcccg gcacggtgga agtcgccggc gacctgggcg ccaccctcga
     2281 cgagcacctg gagcaccccg tcggcaccca actcgtccaa gatgtcgggc agttgaccct
     2341 tcatctcccg gcacggttgg acctgggccg tcgcaggcgc ggtgccgagc acgacccgga
     2401 tcgggtcgac cgatccgctc tcgggcagca ccggcgggtg gtagtcgcgc accgtgagcg
     2461 acggatcgtc gcggcgaacg gtgccggcac cgatgacgat cgcgtcggac tcggcgcgga
     2521 gacgatggcc gtcggcgcgg gcgggagcgc cggtgatcca ctggctggag ccgtccgggg
     2581 ctgcagtggc gccgtccatg gatgccgcca acttcaacac gacccaggga cggcccgtgg
     2641 tgcggtgctt gatgtagggc gccagctgct gacgaacgcg gtcctcgtag aggccgacgt
     2701 cgacggtgat gccggcggca cgcagcttcg cgatgccgtt gccgttgacc cgttcgtcgg
     2761 gatcggcgag cgcgaccacc acacgggcaa cgccggcttc gatcagggcg tcggcgcacg
     2821 gtgccgtgcg accggtgtgc gagcagggct cgagcgtgac gtagaccgtc gcgccctgcg
     2881 ccgcctcgcc cgccgccacg agcgcgacga tctcggcgtg ttcgcggccc acctcccggg
     2941 tggcgccctc ggagatggaa ccgtcggccg ccctcacgac cgcgccgacc cacgggttcg
     3001 gcgacgtggc gcagcgcacc tgcgccgccg cggcgatcgc ccgggtcatg cactcgcggt
     3061 cgagggcgtt gaaggtctca gacatggcgt tcctaccgta tcactgccct ggtcgcaatc
     3121 ctatcggccg ttgagaattc gaccacggcc gtctcttgga gcgctgcaac gcccgctggg
     3181 ggcgccgtgg cgctcgaagg gtcagccgac ggggtgctcg tcgttggcgc cgcccatgag
     3241 gcggatggcg tggggaatga cgtcgaggac ggcgtcgagc atttcgatcg acccgctcgt
     3301 cgagccgggc acgttgagga tgagcgcacg accccgcgtg ccggcgacgc cgcgggacag
     3361 acggccgagc gggttcaccg agcgcatcgc ctcggagagg ccgtaggcac gacgatcgag
     3421 gatgcggacg gtgccctccg gtgtcatgtc gcgggggctg aacccggtgc cgccggtgct
     3481 cacgatgagg ccgaggaagt tgtaggccat gtgagcgagc gcgtgggcca cggtctcgga
     3541 gccgtcggcg cacacgcggt gctccacgat gcggaacccg gcggcggtca ggtgctcgcc
     3601 gatcgccacg ccactcttgt cttcgcgggt gccggccatg acgccgtcgg acacggtgag
     3661 gatcttggcc ttcagcggct tgccgtcagg ggactgggcg gactcgtgat cgtgcggcga
     3721 catggcgacc agcttagaac ggcctcgcaa cgagtcccgc cgacaggtcg ccagcgccgg
     3781 acgctcgccc aaggcaccag agcgcagcca cggcgagctt caggacagcg gccggcgcca
     3841 ggtgaacagt gccatgccgt cgctgtcgtc cgcctgcggc agcaacagcg cgccactccc
     3901 ccacgggatc cacggatcct ccggtggggc cacggcctcg aaccccgggt gggtgtccgc
     3961 gaaccgctcg gccacatcga tcgtctcgga acgggtgagg gtgcacacgc tgtacgcgag
     4021 caggccgccg ggtgcgacga gctcggcgga cgcatcgaga atcgctgact ggatcacgga
     4081 cagctcgtcg acatcgttcg ccgtgatgcg ccagcgggcg tcgggccgac ggcggagcac
     4141 cccgagacct gagcacggcg cgtcgacgag cacgatgtcg gcaacaccgg cacgaagggg
     4201 cgcgtggcgg gcatcggcga cgagcggcca gacagcgccg gagtcggggc gatcggcggc
     4261 ggtacgacgg gcgttgcccg ccacgagacg cacccgcttg gggcgaaggt cggccgcgac
     4321 cacgtgggcg ccggcgccgg ccagcgccgt cgccttgccg ccgggagcgg cgcacacgtc
     4381 gacgaccagg tcaccggccg cggctccgag cgcagcaacg acccgctccg aggagcggtc
     4441 ctgggtgtag ccgtcgtcgc gaacgttgac ctcgggcgcc cggttcatgg tctcgagggc
     4501 gtcggcggcc gcctcgtcac cgaggtcggc gcggaaccgc tcgacgatcc aatcgggata
     4561 gctgagtcgg gttgcgtcgt cgggccagtt caccggggcc ttggcgacct tgcggagcac
     4621 tgcgttgatg aggccccgga accgcttggg agcgacgtcg acggtggccg acacggcggc
     4681 gtgatcagga atgtcggtga accgcagctg atacgcaccc aggcggagtg cggccctggc
     4741 ggccggcggg ggatcgttca cgaggaaccg gtcgacgagg tagtcgagcg atcggcgacg
     4801 gcggatcgtg ccgtacacga gctcggtgac gagtcgacgg tcgcggtcgt cgaggccgga
     4861 cttctcgagg agcggcccga gcctgaggtt ggcgtaggcg ccgtcggcct cgatctcgac
     4921 gagggccgcc agcgccaccg agcggctcgt gaccggcgtc gccggcaccg gggcgctcac
     4981 gagagcccac cgcgtagatc gaccggtgtg ccgcgcagcc agtcctcgta ggccatcggt
     5041 cgtttgcctt cgggctgcac ctcggtgatc cgcagcgaac cgtcggcatc gaggtggcct
     5101 gcaagcacct tcaaccgttt tgcggtttcg ccctcgccga gcatcgtcca ggcgttgccg
     5161 aggcggatga ccgcaagctg ctgctcagcc gacgccgtca ggtcgacgcg ccggtcatcg
     5221 gcggtgagct tggcggcgta ggtgacctcg ccgacctgcg gttcgggggc gatgagccga
     5281 tcaccgtcga acgccgtcgg cagccaatcg acgagcagtt gggagccggt gacgctcaac
     5341 tcgtcccaca actcggtgag cctcttggta ccgaccgggg tcgtcgcgcg ggcgtacaca
     5401 ccgccggtgt cgaggccctc ctcgaccgcc atcacacaca cgccggtctc ggcgtcgccc
     5461 gccagaatgg cgcgttcgac cggggccgcc ccccgccagc ggggcaggag cgagaagtgc
     5521 aggttgacca tcggcacgtg cgccaacagg tgcggacgca ggatcttgcc gaacgcgacc
     5581 accacgccca gcgacgcgtc gctgctgagc accgcgtccg cgtcgtgttc gacccggagc
     5641 cccagc
//
LOCUS       DAOSOA010000218         5601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14609_length_5601_cov_7.243960, whole genome shotgun sequence.
ACCESSION   DAOSOA010000218 DAOSOA010000000
VERSION     DAOSOA010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14609_length_5601_cov_7.243960"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2969)
                     /locus_tag="PKY13_12335"
     CDS             complement(<1..2969)
                     /locus_tag="PKY13_12335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT80772.1"
                     /translation="MAETHLTDDQFLVLVEAHAHGEASPEQVAVLEADHRRWTTSLVH
                     LLADTEDTLEVLRRSNDEQTYQVLTDFENEHRRLLAAYARLTGDVTVLEVDVTEAGLG
                     HPTVALLQLSWEPGKVVAWAAGPTVGAVGADRVAELLVEAGASASNWSKHPVVRLPDG
                     TAAEALTISASEILGWLVALGAEEPGPLVGPSALWLGQVAVWAVDLASRGAMVPLLRQ
                     RRRNRNNTDPNMSSFSVRWTPALVDPVRFNELADAMPGASTAVSPSTDGREVVKSALT
                     GMIDAICRTAAGMIDVPAPPPEVRNGRDTAETFLAKLDGTHFEAPNQTGNELVGRLDR
                     WARPIVTGNPGRLVVQLDPPDSSNAWHLIVLAPDADDQLISVEVALVNAGSQRREIED
                     QLLRLERMLPVLLRPGSNRRGEVVLSQDEAWELMSETGPELSAAGYDVRVPALSTRKA
                     TPSLRLTSEGASSTVVGANQLANVRWSAVFDDVELTAADISRLAKEARPLVRSGGRWV
                     ALDHADLTAAAAALAERSNTTQLTGAEMLRHALGLEGTQLAGGMSLHGSSWAADLMES
                     AKSVATNPVVTPDGFDGDLRSYQSEALGWLGFLDSAGLGGCLALDMGLGKTPTMLAHL
                     LATAGEGPALVIAPPAVVGNWASEAAKFTPKLRVVVHHGASRAVNDEIASEAATADVV
                     ITTYGTAVRDIAAIEKITWDRVVLDEAQAIKNPANDTSQQLRRINARVRVALTGTPIE
                     NGLGDLWAILDFTNPGLVGPRPQFIAQMSGGGTSAKAQGEDALRALNGILVFRRTKAE
                     PAIAAELPDRVDTLDHCSMTPEQIGLYQAVLDRLITHSSEDADPRKGEVLAAITALKQ
                     ICNHPSAYQHDDRPLAGRSGKLARLEEIVHSVFAADEKVLIFTHFAEWGGKMAQHLTA
                     LTGVKVECYHGGLSRGARDDMIANFQAKEGAGALVLSLKAGGTGLNLTAASHVVLYDR
                     WWNPAVEDQARDRAWRIG"
     gene            complement(3182..4180)
                     /locus_tag="PKY13_12340"
     CDS             complement(3182..4180)
                     /locus_tag="PKY13_12340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT80773.1"
                     /translation="MTFPLRLSVLDVAPVLSGSTAREALTNSVDLARHVEALGYERYW
                     LAEHHNTSGIASSVPAVLIGHIAAATSTIRVGSGGVMLPNHAPLHVAESFRLLEELHP
                     GRIDLGIGRAPGTDGLTALALRRSREARSADDFPEQLDELVRFLTDDFPADHPFRDVV
                     ASPQGPSAPSIYLLGSSGYSGALAARLGMGFAFASHINPVPAAKVLRAYQNEFQPSLA
                     FPEPHAILALSAICADDDATAEDLALSLDLTWLRIGQGRGGPLPSVEEARAHDWSEQD
                     LDQRRINRSRHLIGGPAKVQGQIKVRAAEAGVTEVMVLSMIHDHAARRRSYELLTT"
     gene            complement(4225..4788)
                     /locus_tag="PKY13_12345"
     CDS             complement(4225..4788)
                     /locus_tag="PKY13_12345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HOT80774.1"
                     /translation="MDSTLFSTSASSSFGSGLTASSVVVVGNPQPGSRTRSAAELVAR
                     QLAALTSAPDDVLVIELATVGTQLLEWSNQNVAQLKSIVLGAQSLVVSSPTYKASYTG
                     LLKLFVDQFDRDELAGLTTVAVMTGASAAHSLVVETQLKPVLVEIGASLPTRGVYLYG
                     PALDDPTAAVAEWYQSARRPLERIMWR"
     gene            4832..>5601
                     /locus_tag="PKY13_12350"
     CDS             4832..>5601
                     /locus_tag="PKY13_12350"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT80775.1"
                     /translation="MMLPSFSAATAVVPDPDLPGRWSGNVEAGWDVVGNAHGGYLLSI
                     AARALSSATERPDPVTITAHYLSPGRPGPVTVDTTVLRAGRRFATANATVIAGDRPLL
                     AVLGTFGDLGDEVRGPELVSGGPPDLPAPDDCVRVVPGDPFPPAFMAKVDLRLHPDDA
                     RFLGGTRSGTPRIRGWFRLLDDEPMTTNSLLCAVDAFPPTIFNSDLPIGWTPTVELTA
                     HVRARPEPGWMACGFTTMFVSGGFLEADGEIWDRTGRLV"
BASE COUNT          775 a         1783 c         2179 g          864 t
ORIGIN      
        1 ccgatccgcc aagcccggtc acgggcctgg tcctcgacgg cggggttcca ccaccggtcg
       61 tagagcacga cgtggctggc ggcggtcagg ttgaggccgg tgccgccggc cttcagcgag
      121 agcaccagcg cgccggcccc ctccttggcc tggaagttgg cgatcatgtc gtcgcgggcg
      181 ccgcggctga gaccaccgtg gtagcactcg accttgacgc cggtcagggc ggtcaagtgc
      241 tgggccatct tgccgcccca ctcggcgaag tgggtgaaga tcaggacctt ctcgtccgcg
      301 gcgaacaccg agtgcacgat ctcctcgagt cgggccagct tgccggagcg accggcgagc
      361 gggcggtcgt cgtgctggta ggcgctcggg tggttgcaga tctgcttgag ggccgtgatg
      421 gcggcgagca cctcgccctt gcgagggtcg gcgtcctccg acgagtgcgt gatgagccgg
      481 tcgaggacgg cctggtagag gccgatctgc tcgggggtca tcgagcagtg gtcgagggtg
      541 tcgacgcggt cggggagctc ggcggcgatg gccggctcgg ccttggtgcg gcggaacacc
      601 aggatgccgt tgagcgcccg gagggcgtcc tcgccctgtg ccttggcgga ggtgccgccg
      661 cccgacatct gggcgatgaa ctgcgggcgg gggccgacga gaccggggtt ggtgaagtcg
      721 aggatggccc acaggtcgcc gagcccgttc tcgatggggg tgccggtgag ggcaacgcgc
      781 acacgggcgt tgatgcggcg gagctgctgg ctcgtgtcgt tggccgggtt cttgatcgcc
      841 tgcgcctcgt cgaggacgac gcggtcccac gtgatcttct cgatggcggc gatgtcgcgg
      901 accgccgtgc cgtaggtcgt gatcacgacg tcggcggtgg cggcttccga ggcgatctcg
      961 tcgttgacgg cgcgggacgc gccgtggtgc acgacgaccc ggagcttggg tgtgaacttc
     1021 gcggcctccg acgcccagtt gccgacgacg gcgggcgggg cgatgacaag ggcggggccc
     1081 tcaccggcgg tggccaggag gtgggcgagc atggtcggcg tcttgccgag ccccatgtcg
     1141 agcgcgaggc agccgccgag accggcgctg tcgaggaacc cgagccagcc gagcgcctcg
     1201 ctctggtagc tgcggaggtc gccgtcgaac ccgtcggggg tgacgacggg gttggtcgcg
     1261 accgacttgg ccgattccat gaggtcggcg gcccagctcg agccgtgcag cgacatgcca
     1321 ccggcgagtt gggtgccctc gagaccgagg gcgtggcgca gcatctcggc acccgtgagc
     1381 tgcgttgtgt tcgagcgctc ggcgagggcc gccgccgcag cggtgaggtc ggcgtggtcg
     1441 agcgctaccc agcgcccgcc ggaccggacg agcggacgtg cttccttggc aaggcgggag
     1501 atgtcggcgg cggtgagctc gacgtcgtcg aacacggccg accaacggac gttggcgagc
     1561 tggttcgcac ccacgaccgt ggagctggcg ccctcggacg tgagccggag cgacggtgtg
     1621 gccttgcggg tcgacagcgc ggggacccgc acgtcgtagc cggcggccga cagctcgggg
     1681 ccggtctccg acatgagttc ccaggcttcg tcctggctga ggacgacctc gccgcggcgg
     1741 ttgctgcccg gacggaggag gaccggcaac atccgttcga gccggaggag ctggtcctcg
     1801 atctcgcggc gttgtgaacc ggcgttgacg agcgcgacct cgaccgagat gagttggtcg
     1861 tcggcgtcgg gggccaggac gatgaggtgc caggcattcg acgaatccgg cggatcgagc
     1921 tgcaccacga gacggccggg gttgcccgtg acgatcgggc gggcccagcg gtcgagccgg
     1981 ccgaccagtt cgttgccggt ctggttggga gcctcgaagt gggtgccgtc gagcttcgcg
     2041 aggaacgtct cggcggtgtc gcggccgttg cggacctcgg gcgggggagc gggcacgtcg
     2101 atcatgccgg cggcggtccg gcagatcgca tcgatcatgc cggtgagtgc cgacttcacg
     2161 acctcgcggc cgtcggtcga gggggacacc gcagtggaag cgccgggcat ggcgtcggcg
     2221 agctcgttga atcgcaccgg gtcgacgagg gcgggggtcc agcgcaccga gaacgagctc
     2281 atgttcggat cggtgttgtt gcggttgcgg cgacgctggc ggaggagcgg gaccatggcg
     2341 ccgcggctcg cgaggtcgac cgcccagacg gcgacctggc cgagccagag tgcgctcggc
     2401 ccgacgagcg ggcccggttc ctcggcgccg agcgcgacga gccagccgag gatctcgctc
     2461 gcgctgatgg tgagcgcttc ggcagcggtg ccgtcgggca gccgcacgac ggggtgcttc
     2521 gaccagttcg aggccgacgc gccggcctcg acgagcagtt cggcgacacg gtcggcgccg
     2581 acggcaccca cggtgggccc ggctgcccag gcgacgacct tgccgggttc ccaggacagc
     2641 tgcagcagcg ccaccgtggg gtggccgagc ccggcctcgg tcacgtcgac ctcgaggacg
     2701 gtgacgtcgc cggtcaggcg ggcgtacgcg gcgaggaggc gacggtgttc gttctcgaag
     2761 tccgtcagca cctggtaggt ctgctcgtcg ttggaccgtc ggagcacctc gagggtgtcc
     2821 tcggtgtcgg cgagcaggtg cacgagcgac gtggtccacc gacggtggtc ggcttcgagg
     2881 acggcgacct gttcgggcga ggcctcgccg tgcgcgtggg cttcgacgag caccaggaac
     2941 tggtcgtcgg tcagatgggt ttcggccatg ttcggtggca cgctccgctt cgcatgtgat
     3001 gaggacgccg acgggcggac cttgttgggc aaggttccgt gcgtcgggcg tgtggatggc
     3061 tccaaccccg caatccgtgt ggccggagcc gtgctgatcg cccatgcccc ccgccgactg
     3121 cccggagtgg ggccgggtca cgagctcctg cccgtcgacc gctcgaccac tctagcgctg
     3181 atcaggtggt gagcaactca tagctccgcc gacgcgcggc gtggtcgtgg atcatcgaga
     3241 ggaccatgac ctcggtgacg ccggcctcgg cggcgcggac cttgatctgc ccctggacct
     3301 tcgccggccc gccgatcagg tgccgcgagc ggttgatacg tcgttggtcg agatcctgct
     3361 cggaccagtc gtgggcgcgc gcctcctcga cgctcgggag cgggccgccg cggccctgcc
     3421 cgatgcggag ccacgtcagg tcgagcgaga gcgccaggtc ctccgcagtc gcgtcgtcgt
     3481 cggcgcagat ggcggagagg gcgaggatcg cgtgcggttc ggggaacgcc agggacggtt
     3541 ggaactcgtt ctggtaggcg cggagcacct tcgccgcagg aaccgggttg atgtggctgg
     3601 cgaaggcgaa ccccatgccg aggcgcgccg ccagcgcccc gctgtatccg ctcgatccga
     3661 ggaggtagat ggaaggagcc gacggaccct gcggcgaggc gaccacgtcg cggaaggggt
     3721 ggtcggcggg gaagtcgtcg gtcaggaacc tgacgagctc gtcgagttgt tccgggaagt
     3781 cgtcggcgga gcgggcctcg cgggatcgcc gcagtgcgag cgccgtgagg ccgtcggtgc
     3841 cgggagccct gccgatgccg agatcgatgc gcccggggtg gagctcctcg agcagccgga
     3901 acgactcggc gacgtggagc ggggcgtggt tgggcagcat gacgccaccg gagccgacgc
     3961 ggatcgtgga ggtcgcagcg gcgatgtggc cgatcaggac ggcgggaacc gagctggcga
     4021 tccccgaggt gttgtggtgc tcggccagcc agtaccgctc gtatccgagg gcttcgacgt
     4081 ggcgggcgag atcgacggag ttggtgagcg cctcgcgtgc ggtcgaaccc gagagcacgg
     4141 gggcgacgtc cagcacggag agccggaggg ggaaggtcat cgagttggtc gaaccggggt
     4201 cgggcggcgg ctattccgga gggttcagcg ccacatgatg cgttcgagcg ggcggcgagc
     4261 ggactggtac cactcggcca ccgctgcggt tggatcgtcg agtgcggggc cgtacaggta
     4321 gaccccgcgg gtcgggaggc tggcaccgat ctcgacgagc acgggtttga gctgcgtctc
     4381 gacgacgagc gagtgggcgg cgctcgctcc ggtcatcacc gccacggtcg tcagtcccgc
     4441 gagttcgtcg cggtcgaact ggtcgacgaa cagcttgagt agacccgtgt agctggcctt
     4501 gtacgtcggg ctcgacacga cgagggactg tgcgccgagc acgatcgact tcagctgagc
     4561 cacgttctgg ttgctccact cgagcagttg ggtgccgacg gtggcgagct cgatcaccag
     4621 gacgtcgtcg ggtgcgctgg tgagcgcggc gagctgccgt gcgacgagct cggcggccga
     4681 gcgcgtgcgc gaaccaggct ggggattgcc gacgacgacg acggaggagg ccgtgagacc
     4741 ggacccgaac gacgacgagg cggaggtcga gaagagggtg gagtccaagg cgttggacac
     4801 tatcggcgcc ggtgtgatcg cgtgccggcc gatgatgttg ccctcgttct ccgccgccac
     4861 cgccgtcgtt cccgaccccg acctccccgg ccggtggtcc ggcaacgtcg aggccggctg
     4921 ggacgtcgtc ggcaatgccc acggcggata cctgttgtcg atcgctgccc gagctttgtc
     4981 gtcggcgacc gagcggccgg atccggtcac gatcacggcg cactacctct cgccggggcg
     5041 gccaggtccg gtcacggtcg acacgaccgt gctgcgcgcc ggtcgccgct tcgcaacggc
     5101 gaacgcgacg gtgattgccg gcgaccggcc gctccttgcc gtcctcggca cgttcggcga
     5161 cctcggcgac gaggttcgag gccccgagct cgtctcggga gggccgcccg atcttccagc
     5221 gccggacgac tgcgtgcggg tcgtccccgg tgacccgttc ccgccagcgt tcatggccaa
     5281 ggtcgacctc cgtctccatc ccgacgacgc ccggttcctc ggcggcacga ggagcgggac
     5341 gccgcgcatc cgcggctggt tccgcttgct cgacgacgag ccgatgacga ccaactcgct
     5401 cctgtgcgcg gtcgatgcct ttccgccgac gatcttcaac tccgacctgc cgatcggttg
     5461 gacgccgacg gtcgagctga cggcccacgt ccgggcccgg ccggaaccgg ggtggatggc
     5521 gtgcgggttc accaccatgt tcgtgagcgg tggcttcctc gaggccgacg gcgagatctg
     5581 ggaccgaacc ggtcggctcg t
//
LOCUS       DAOSOA010000219         5570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14718_length_5570_cov_5.191840, whole genome shotgun sequence.
ACCESSION   DAOSOA010000219 DAOSOA010000000
VERSION     DAOSOA010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14718_length_5570_cov_5.191840"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKY13_12355"
     CDS             complement(<1..283)
                     /locus_tag="PKY13_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80776.1"
                     /translation="MSPSGSSTSPSSASFPERRLRRLRRTPALRRLVAETTLTPNDLI
                     APLFVREGIDEPQPISSLPGVVQFDLASLETEVSELVALGVPAVILFGVP"
     gene            478..1680
                     /locus_tag="PKY13_12360"
     CDS             478..1680
                     /locus_tag="PKY13_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leishmanolysin-related zinc
                     metalloendopeptidase"
                     /protein_id="HOT80777.1"
                     /translation="MVGCLAAGCVPPASGGTGSNNGGGGNGGTVTPTTAASTPPTSPS
                     ATNPGSPVIDAFSATTTAAASPLTTALRWTIHDPSGDALTCTLDLDGNGSVDVTISPC
                     TSDSSRTRTFTAVGTNAVTLAVTDGASTTASTIALNVAAPSADQYSVTLRFTGTMTGG
                     QTAAFTTAAAKWASIIKTGQPDRTVSIGADYCTSGTAAYSGVVDDLLIDAQIVPIDGA
                     GGILGQAGPCLVRASNGLPLYGIMQFDSADVTGLESSGQLANTIIHEMGHIVGIGTVW
                     TNKSVLADAGTSNPTYTGPAAIGAWRELGGSGNVPVENGGGPGTADSHWRETTFRTEL
                     MTGYLNSSNQLSAMTAASLADLGYGVDIAGADAYSLPALRAASVQADGVHDHNHEDFI
                     TVLPIGSA"
     gene            complement(1685..2692)
                     /locus_tag="PKY13_12365"
     CDS             complement(1685..2692)
                     /locus_tag="PKY13_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT80778.1"
                     /translation="MNGTSAAMTGSTNLLGWTLRGAAGVALIPMIIVTVWIWFGWSRP
                     SPVLVLEGFQMYLLAPAWVILIATLATRQWALAVPAVFVAGSHLLFCLPAATADSVPA
                     WASDAPTMTVFVANVRYDNARADELARDVMASNADVVVLNETTPAIRDALRAAGTSDR
                     YPTRVHVEGRPFGETLMTRLPATDAGVEVLGGQRVPAATVTVGDRQVRVYSVHVNAPK
                     SSAQRHIWRRNLDGIGGSAEAAGDVPHLYAGDFNSAPWHGPYRRLLARGLTDAHDALG
                     QGLSRSWTPHPAVLSWLGPVMRLDHALFTPGLFPRRLADVEVPGSDHRGFLLEMAVRQ
                     P"
     gene            complement(2689..>3636)
                     /locus_tag="PKY13_12370"
     CDS             complement(2689..>3636)
                     /locus_tag="PKY13_12370"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOT80779.1"
                     /translation="MGVANIGRISERLIAGGLAPQTPVAAVRWGTRPNQHTTRASLAT
                     IAEHPLAAPSVIVIGDVAADEFEWFAGRPLTGRRIVVTRAPAQASRLTDRLLAAGADV
                     INVPTIAIADPPDGGAALAAAAAAVSTYDWVVLTSPNGAERFLDAVGDARRLAGVRLA
                     VIGPGTAAVLRAHRMEPDLIPARYVAEGLLEDFPDGPGRVLIPQAREARPVLAEGLLG
                     RGFVVDVVPAYETVPADVPAGALADIAGADAVAFASSSAVTNFVAAVPEASRPQVAVT
                     IGPISAATARDAGFEVVEAAQHDLDGLVAAVLEWATATS"
     assembly_gap    3637..3736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3737..3951)
                     /locus_tag="PKY13_12375"
     CDS             complement(<3737..3951)
                     /locus_tag="PKY13_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT80780.1"
                     /translation="MTVFLVGAGPGDPGLITVRGAELLARADVVIYDRLSAPSLLELV
                     PESAQRIYVGKRGSEGSTPQERINELLV"
     gene            complement(3956..4846)
                     /gene="hemC"
                     /locus_tag="PKY13_12380"
     CDS             complement(3956..4846)
                     /gene="hemC"
                     /locus_tag="PKY13_12380"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOT80781.1"
                     /translation="MSDSASLRRIATRGSALALWQAHHVAGLLADVAPEITVEIEIIS
                     TEGDRRTDVPLAQIGGKGVFVKEVQAAVLDGRADIAVHSAKDLPALTPTGLVIVAVPE
                     RADARDVLVGGRLADLPAGATVATGSPRRRAQLADLRPDLRFEELRGNIDTRLAKSSS
                     FDAIVMAKAALDRLDRHPEVVDPIDVDVMVPQVGQAALAIECRADDHHLVELLSRLDH
                     RATRLAVDAERAFLARLGGDCDLPAGAHATVADDDTITLTGVLLDAGDTTMRRFASVG
                     QVPDELGALVAERLLSGTGT"
     gene            complement(4850..>5570)
                     /locus_tag="PKY13_12385"
     CDS             complement(4850..>5570)
                     /locus_tag="PKY13_12385"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80782.1"
                     /translation="LDGRGVVVVGAGSMARGVVDFVSSRSARQMTVLNRTPERARVLA
                     AEVHHWDGLHELPTCLADADVVFCATSSPASVIDANMVRSAMAERAGRPLLLVDIAMP
                     RDVAPEVAGLDDVTLLDMDGLSAFAERGLSRRRQEIPAVEAIVDDEVDRYLAASSARE
                     VAPLIVELRRRGDSVVADELQRHAAKLAVLDPAQREIVESVLRGAVAKLLHEPTVALK
                     DAAGSVRGERLVGSLRELFDL"
BASE COUNT          891 a         1932 c         1943 g          704 t
ORIGIN      
        1 acggcacccc gaacaggatc accgcaggca cacccaacgc caccagctca ctcacctcgg
       61 tctcgaggga tgcgagatcg aactggacga ccccgggcaa cgacgagatc ggctggggct
      121 cgtcgatgcc ttcgcggacg aacagcgggg cgatcaggtc gttcggcgtc agcgtcgtct
      181 cggcgacgag gcggcggagc gccggggtac ggcggagccg acggagacga cgctctggaa
      241 agctcgctga cgacggggaa gtggaggacc cggatggaga cacggccgga gtctacggag
      301 cggaccgacc ccggcgaacc cgcggcctgc ggagacgtgc ggggaccgcg tcgcggaggt
      361 gccggagccg agccagcgcc ggaggtccga ttgcctcggc aatcgttaca ggtcctcccc
      421 cgaatcgacg acaacctctc catgaagagc tcgctgggtg cgatcgtagg aacgttgatg
      481 gtcggctgcc tggcggcggg gtgcgtaccg cccgcctccg gcgggaccgg gtcgaacaac
      541 ggtggcggcg gaaacggcgg gaccgtgacg ccgaccacgg cggcgtcgac gccaccgacg
      601 tccccatccg ccacgaaccc cggctccccg gtcatcgacg ccttctcggc gaccacgacc
      661 gcagccgcct ccccgctgac gaccgccttg cgctggacca tccacgatcc gagcggcgac
      721 gcgttgacct gcacgctcga cctcgacggc aacggctccg tggacgtcac gatcagcccc
      781 tgcacgagcg actcctcccg cacacggacg ttcaccgcgg tcggcaccaa cgcggtcacg
      841 ctcgccgtca ccgacggcgc ctcgaccacg gcgtcgacga tcgcactcaa cgtcgccgcg
      901 ccgtccgctg accagtactc cgtgaccctg cggttcacgg gcacgatgac cggtggccag
      961 acggcagcct tcacgaccgc cgctgcgaag tgggcgtcga tcatcaagac cgggcaaccg
     1021 gaccgcacgg tctcgatcgg cgccgactat tgcaccagcg gcacggccgc ctacagcggt
     1081 gtcgtcgacg atctgctgat cgacgcccag atcgtgccca tcgacggagc cggcggcatc
     1141 ctcggccagg ccgggccctg cctggtcagg gcgtcgaacg gtctccccct ctacggcatc
     1201 atgcagttcg acagcgccga cgtcaccggt ctcgagtcct ccggacagct cgcgaacacg
     1261 atcatccacg agatgggtca catcgtcggc atcggcacgg tgtggacgaa caagtcggtc
     1321 ctcgccgacg ccggcacctc caacccgacc tacaccggcc cggcggcgat cggcgcgtgg
     1381 cgtgagctcg ggggcagcgg caacgtgccc gtcgagaacg gcggcgggcc gggcaccgcc
     1441 gactcccact ggcgtgagac gacgttccgg accgagctga tgaccggata cctgaactcc
     1501 tcgaaccagc tgagcgccat gaccgcggcg tcgctcgccg acctgggcta cggcgtcgac
     1561 atcgccggag ccgacgccta ctcgttgccg gcactgcgtg ctgcctcggt gcaggcggac
     1621 ggggtgcacg accacaacca cgaggacttc atcaccgtcc tgccgatcgg gtcggcgtag
     1681 gaactcaggg ttgtcggacg gccatctcga ggaggaagcc tcggtggtcg ctgccgggga
     1741 cctcgacgtc ggcgagccga cgggggaaca gccccggcgt gaacagtgcg tggtcgagcc
     1801 gcatcaccgg gccgagccat gacagcacgg cgggatgggg cgtccacgat cgactgaggc
     1861 cctggcccag cgcatcgtgc gcatcggtca ggccacgggc gagcagccga cggtacggtc
     1921 cgtgccacgg ggcggagttg aagtcgcccg cgtagaggtg cggaacgtcc cctgcggctt
     1981 ctgccgaccc accgatgccg tcgaggttcc gacgccagat gtgccgttgg gccgacgact
     2041 tcggggcgtt cacgtggacc gagtacacgc gcacctgacg gtcgccgaca gtgacggtcg
     2101 ccgccggcac acgctggccg ccgagcactt ccaccccagc atcggtcgcc gggagccgcg
     2161 tcatgagcgt ctccccgaac ggtcgaccct cgacgtgcac ccgcgtgggg taccggtcgc
     2221 tcgtgccggc agcgcggagt gcgtcgcgga tcgccggcgt cgtctcgttc agcaccacga
     2281 cgtcggcatt cgaggccatc acgtcgcggg ccagctcgtc ggcccgggcg ttgtcgtacc
     2341 gaacgttcgc aacgaagacg gtcatcgtcg gggcgtcgga cgcccaggcg ggaacgctgt
     2401 ccgccgtcgc agcggggagg cagaagagga gatgggaccc ggcgacgaag accgccggga
     2461 ccgcgagcgc ccactgccga gtggcgagcg tcgcgatcag gatgacccac gccggtgcca
     2521 gcaggtacat ctggaaaccc tcgagcacga gcaccggcga cggacggctc cacccgaacc
     2581 agatccatac cgtgacgatg atcatcggga tcagcgcgac cccggctgca ccgcgaagcg
     2641 tccaacccag caggttcgtc gagccggtca tcgctgcgga cgtgccgttc atgacgtcgc
     2701 ggtggcccac tccagcaccg ccgcgacgag accgtcgaga tcgtgctgtg cggcctccac
     2761 gacctcgaag cccgcatcac gtgccgttgc cgccgagatc ggcccgatcg tcacggcgac
     2821 ctggggccgg gatgcctcgg gcaccgccgc gacgaagttc gtcaccgccg acgaactcgc
     2881 gaacgccacg gcgtccgccc ccgcaatgtc ggccaacgca ccggccggaa cgtcggcggg
     2941 gacggtctcg taggcgggca cgacgtcgac gacgaagccg cgcccgagga gcccctcggc
     3001 gagcaccggc cgcgcctcgc gggcttgggg gatcagcacc cgtccggggc cgtcggggaa
     3061 gtcctcgagg agaccctcgg cgacatagcg cgccgggatc aggtcgggct ccatccggtg
     3121 cgcgcgcagc accgcggcgg tgccgggacc gatgacggcg agacgaaccc cggccaggcg
     3181 tcgggcgtcg ccgacggcgt cgaggaaccg ctccgcaccg ttgggtgatg tgaggacgac
     3241 ccagtcgtac gtcgagaccg ccgcggccgc cgccgcgagc gccgctcccc cgtcgggcgg
     3301 atcggcaatg gcaatcgtcg gcacgttgat cacgtcggcg cctgctgcca gcaggcggtc
     3361 ggtcagacga gatgcctgcg caggcgcccg cgtcacgacg atgcgccgac ccgtcagcgg
     3421 ccgcccggcg aaccactcga actcgtcggc ggcgacatcg ccgatgacga tcaccgacgg
     3481 ggctgcgagc ggatgctcgg cgatcgtggc gagcgatgcc cgggtcgtgt gctggttggg
     3541 gcgggtcccc catcgcacag ccgcgacggg cgtctggggt gcgaggccgc cggcgatcag
     3601 gcgctcgctg atccgaccga tgttggcgac ccccatnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnacca gcagctcgtt gatgcgctcc tgtggggtgg agccctcgga
     3781 tccgcgtttg ccgacgtaga tccgttgcgc cgactccggc acgagctcga gcagcgacgg
     3841 agccgacaac cggtcgtaga tcaccacatc ggcccgggcc aacagctcgg cgccgcggac
     3901 cgtgatcagt cccgggtcgc cggggcccgc gccgaccagg aacacggtca cggtgtcagg
     3961 tacccgttcc cgagagcagg cgctcggcga cgagggcgcc caattcgtcg ggcacctggc
     4021 cgaccgaggc gaaccggcgc atcgtcgtgt cgccggcgtc gagcagcact ccggtgaggg
     4081 tgatcgtgtc gtcgtcggcg accgtcgcgt gggcgccggc gggcagatcg cagtcgccgc
     4141 cgaggcgggc gaggaaggca cgttccgcat cgacggcgag acgcgtcgcg cggtggtcga
     4201 ggcgggagag cagctcgacg agatggtggt cgtcagcgcg gcactcgatg gcgagggcag
     4261 cctgaccgac ctggggcacc atgacgtcga cgtcgatcgg atcgacgacc tcggggtggc
     4321 gatcgagacg gtcgagcgcc gccttggcca tcacgatcgc atcgaacgag gacgacttgg
     4381 cgagccgggt gtcgatgttg ccgcgaagct cttcgaaccg gagatcgggg cgcaggtcgg
     4441 ccagctgggc acgacgccgc ggcgacccgg tggcgaccgt tgctcccgcc gggagatcgg
     4501 cgagccgtcc gccgacgagc acgtcgcggg cgtccgcacg ctcggggacg gcgacgatca
     4561 ccagaccggt cggggtgagg gcgggaaggt ccttcgccga gtgcacggcg atgtcggcgc
     4621 gaccgtcgag gactgctgcc tgcacctcct tgacgaacac gcccttgcca ccgatctgcg
     4681 ccaacggcac gtcggtgcgg cgatcgccct cggtcgagat gatctcgatc tccaccgtga
     4741 tctccggcgc gacgtcagcg agcaggccgg cgacgtgatg ggcctgccac agggcgaggg
     4801 cggagccccg ggtggcgatg cgtcgcagcg acgcggaatc cgacaccgct tacaggtcga
     4861 acaactcgcg cagcgagccc acgagccgtt cgccacggac ggagcctgcc gcgtccttga
     4921 gcgccaccgt cggctcgtgc aacagcttgg cgacggcgcc gcgcagcaca ctctcgacga
     4981 tctcccgctg ggcagggtcg aggacggcca gcttggcggc gtgacgctgt agctcgtccg
     5041 ccaccaccga gtcgccccgt cggcggagct cgacgatgag cggcgcgacc tcgcgcgccg
     5101 agctggcggc gagatagcgg tcgacctcgt cgtcgacgat ggcttcgacc gctggaatct
     5161 cctggcggcg acgactcaga ccccgttcgg cgaacgcgct caggccgtcc atgtcgagga
     5221 gcgtgacgtc gtcgagaccg gcgacctccg gagcgacgtc gcgcggcatg gcgatgtcga
     5281 cgagcaggag tggacgaccg gcccgttcgg ccatcgccga gcgcaccatg ttggcgtcga
     5341 tcacactggc cggcgaggac gtggcgcaga agaccacgtc ggcatcggcc aagcaggtcg
     5401 gcaactcgtg gagaccgtcc cagtggtgca cctcggcggc gaggacccgg gcacgttcgg
     5461 gagtgcggtt gagcaccgtc atctgccgtg cggagcgcga cgagacgaag tcgacgaccc
     5521 cgcgcgccat cgacccggcg ccgacgacga ccaccccgcg accgtcgaga
//
LOCUS       DAOSOA010000220         5540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14805_length_5540_cov_6.736737, whole genome shotgun sequence.
ACCESSION   DAOSOA010000220 DAOSOA010000000
VERSION     DAOSOA010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14805_length_5540_cov_6.736737"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2092
                     /locus_tag="PKY13_12390"
     CDS             <1..2092
                     /locus_tag="PKY13_12390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT80783.1"
                     /translation="PASPLTRVAAEQLRDVISVEVVPIAAGDPVLSARTQLLPAQNRL
                     VEGAGQDGLAGVDASADRAAEHLYVLGIATDVAVAAAANRPDSSSAWTSFGIAIRTLG
                     GAGGLGVLTAGPLSGWVAGPGAAERLTELIDPLVEELGSRAPSPDYLGTVAWSLDQLD
                     LVRGAEPPVAAPSTEAGLAGLLGGIDPGLITSVLGALAPADGVGPPLAVDEALDETER
                     LVGEITRLGASQSSDRSRALHDSAAESRNTATLLLLMAAAVVGAAVILLLLLVVAARR
                     AQRRSEQDSLEDVLTGMLNRGGLFSSLVDLRADDGMWVVLVDLDRFKPVNDSYGHAVG
                     DLLLVAVAGELESAAAPLEGLVARLGGDEFAIVLGGATAHDVDLAVARIIERLEVVTV
                     DGRRIELGCSVGVVHGAPGVGIDQLLTEADLAMFRAKRSGRGRSVHFDEATRAALASF
                     RDGTIGDTIDIGVRLQRSLVDRSIVGAMVVPQVPVGGGDPIEGSELRLIAEFAGRRFG
                     LLDATLAGIGRSGRTIPPAGIRLWFEVSAVDVVTYGGVEALWERLTTVGLSGAPFGVE
                     LVDAGTQDPDALAAAVATLQELGVHVAVWSTGPGAVALTGLVRLGADRLVVDPSVVEA
                     IRGGDVELSRAVGSVTGLGAALGSEVIATGVPSLDVAHTLVELGVGVAQVMVTEPWAP
                     KRPLKRGDSVVVDR"
     gene            complement(2112..3152)
                     /locus_tag="PKY13_12395"
     CDS             complement(2112..3152)
                     /locus_tag="PKY13_12395"
                     /inference="COORDINATES: protein motif:HMM:NF039286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOT80784.1"
                     /translation="MKRSPFPVPYGWFQVGWATDVAIGETKPLRYFGKHLVLWRDEDG
                     GVHVQDAFCPHLGAHLGHGGKVLKCEIACPFHGWRFDAQGANTLIPYSKRTNAKGKLR
                     TFPTIERNGLIMTWYHPDGVEPMWEVPIVPEFNDPDQFTEMVTRIYQVAAPWQELAEN
                     GVDAAHFRYVHNTEEVPELAGYDTEGPIARMKSIQKFPTPRGVVDGYINSDSFGPGLS
                     VVHFGGIVDTILMGCNTPIDDEHCEMRFSFTVRKLGDAGFDTTVGDAFVAEVDRQVRE
                     DKPIWENKAYLPRPALADTDGPVTKFRKWASQFYAEGVVDSQDFYPPSDSYPAEDLGL
                     SASQKYGSAPIA"
     gene            complement(3250..4158)
                     /locus_tag="PKY13_12400"
     CDS             complement(3250..4158)
                     /locus_tag="PKY13_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT80785.1"
                     /translation="MPATLLPQPTGRFGSARNRHRLLVVVAMTLTSFALASWPARPAG
                     AAPSAAAKVVDSGRLSGLRYHLVAPGEGLKGIAKTYGIPVAQIRSANGMTKDTVYAGA
                     RLLLDEPNPGRMAPPAGSGPTSTYTVVAGDTVARIAKGHGTTTDAIVAANQLKSANQI
                     RIGQVLTISGGQTPGMVCPVAGSTYAFDWGFPRADGARFHEGIDMMAPAGTPILAPTD
                     GKVTYGSSNVSGKFATLKGSNGWQYYSAHLSKTAKGGKVAAGDVIGYVGNTGDAKGGA
                     PHLHLEIRPLDGRPVNPYPVITAACG"
     gene            complement(4256..5122)
                     /locus_tag="PKY13_12405"
     CDS             complement(4256..5122)
                     /locus_tag="PKY13_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT80786.1"
                     /translation="MGMPTDIGVIDLMLGIPDPGKKEGWYDFLKPNLRDAESATFKFP
                     AQYMFKEVPEDPTEGDMVEYTIDQLNKHGIEKALIGINLAGGIQLDACIEHPDRFVAG
                     LAVDPNEGMKAIHKINEAAQRVDLKAITLFPAGCNPGVPINDKRMYPVYAKACELDIP
                     VLCCVGVPGPRVPMMPQHVELIDEVCWFFPELKFVMRHGADPWTDLAVKLMLKWPNLY
                     YSTSAFAPKHYPEEIIRFANTRGADKIMYAGYFPMGLSLDRIFSEMPNVGFKDEVWPK
                     FLRENAKRVFNL"
     gene            complement(5179..>5540)
                     /locus_tag="PKY13_12410"
     CDS             complement(5179..>5540)
                     /locus_tag="PKY13_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT80787.1"
                     /translation="LYQAFLHNPGGLWDADVLADATGHIRTTLPDPILRTPANRTLGL
                     IAAGDDGTSSFRGMGHNVSARAFGHNGAAGQIAWADPETGLSVCYLTNGVDRNFLREA
                     RRVSGIASRAALLTKSS"
BASE COUNT          783 a         1764 c         2047 g          946 t
ORIGIN      
        1 gccggcgtcg ccgctgacgc gggttgcggc cgaacagctc cgcgacgtca tctcggtgga
       61 ggtcgtcccg atcgctgccg gtgacccggt gttgtcggct cgaacccagc tcctcccggc
      121 gcagaaccgc ctcgtcgaag gcgcgggcca ggacggtctg gccggggtgg acgcgtcggc
      181 cgatcgagct gccgagcacc tctacgtgtt ggggattgcg accgacgtcg cggttgccgc
      241 cgccgccaac cggccggatt cgtcgtcggc gtggacctcg ttcggaatcg cgatccgaac
      301 gttgggtggg gcgggcgggc tgggcgtgct gaccgccgga ccgctgtcgg gatgggtggc
      361 cggtccgggc gcagcagagc gtctcaccga gttgatcgac cccctggtcg aggagttggg
      421 gagccgggcg ccgagccccg actacctggg gaccgtcgcc tggtcgctcg accagctcga
      481 tctggtccgt ggagcggagc cgccggtcgc agcgccgtcg accgaggcgg gactcgccgg
      541 cttgctcggc ggaatcgacc ccgggctcat cacgtcggtg ctcggagcgt tggcgccggc
      601 cgacggggtc ggtccgccgc tcgccgtcga cgaagcgctg gacgagaccg aacgtctggt
      661 cggggagatc acccgcctgg gcgcgagcca gtcgtcggac cggtcgcggg cgcttcacga
      721 cagtgctgcc gagagccgca acactgcgac gctgctgttg ctcatggcgg cggctgtcgt
      781 gggagcggcc gtgatcctgc tcctgctgct cgtggtcgcg gcacggcggg cgcagcggcg
      841 gagcgagcag gactccctcg aggacgtcct gaccgggatg ctcaaccgcg gtggactctt
      901 ctcgtcgctc gtggacctcc gtgccgacga cgggatgtgg gtcgtcctcg tcgacctcga
      961 ccggttcaaa ccggtgaacg acagctacgg gcacgcggtc ggcgacctcc tcttggtggc
     1021 ggtggccggt gagctcgagt cggccgccgc gccgctcgag ggcctcgtcg cccgactcgg
     1081 cggcgacgag ttcgcgatcg tcctcggcgg ggcgaccgct catgacgtcg acctggcggt
     1141 cgcccggatc atcgaacgcc tcgaagtcgt caccgtcgac gggcgacgca tcgagctggg
     1201 gtgcagcgtg ggtgtggtgc acggcgcgcc cggggtcggc atcgatcagc tgctcaccga
     1261 ggccgatctc gcgatgttcc gggccaaacg atcgggccgc gggcggtcgg tccacttcga
     1321 cgaggcgacc cgtgccgcgc tggcgagctt ccgtgacggc acgatcgggg acacgatcga
     1381 catcggcgtc cggttgcagc gctcgttggt cgaccgttcc atcgtcggtg ccatggtcgt
     1441 gccccaagtc ccggtgggcg gaggcgaccc gatcgagggt tcggaactcc gactcatcgc
     1501 cgagttcgcc ggccgcaggt tcggtctgct cgacgcgacg ctggccggga tcggccgttc
     1561 cggccggacg atccctccgg ccgggatccg gctctggttc gaggtgtccg cggtggacgt
     1621 cgtgacctac ggaggggtgg aggccctgtg ggagcggttg accaccgtcg gactgtccgg
     1681 cgcgccgttc ggcgtcgaac tggtggacgc cggaacgcag gatcccgatg ccttggcggc
     1741 cgccgtcgcc accctgcagg agctcggcgt gcacgtggcg gtgtggtcga cgggtcccgg
     1801 ggcggtcgcg ttgaccggcc tggtccggct cggcgccgac cgcctggtgg tcgacccgag
     1861 cgttgtcgaa gcgatccgag gcggcgacgt cgaattgtcc cgcgccgttg gctcggtcac
     1921 cgggctgggc gcggcgctcg gatccgaagt gatcgcgacc ggcgttccgt cactcgacgt
     1981 ggcgcacacc ctcgtcgagc tgggggttgg cgtcgcgcag gtgatggtca ccgagccgtg
     2041 ggcgccgaag cgcccgctca agcgcggtga ctcggtcgtt gtggaccgat aggacctgac
     2101 gtggcggtcg ctcaggcgat cggcgcggaa ccgtacttct ggctggcaga caggccgagg
     2161 tcctcggcgg ggtagctgtc gctcggcggg tagaagtcct gggaatcgac gacgccctcg
     2221 gcgtagaact gcgacgccca cttgcggaac ttggtcacgg ggccgtcggt gtcggccagc
     2281 gccgggcggg gcaggtacgc cttgttctcc cagatcggct tgtcctcgcg gacctgccgg
     2341 tcgacctcgg cgacgaacgc atcgccgacc gtcgtgtcga agccggcgtc gccgagcttg
     2401 cgcaccgtga agctgaaccg catctcgcag tgctcgtcgt cgatgggtgt gttgcagccc
     2461 atgaggatgg tgtcgacgat gccgccgaag tggacgaccg agaggccggg gccgaacgaa
     2521 tcggagttga tgtaaccgtc gacgacgccg cggggtgtgg ggaacttctg gatcgacttc
     2581 atgcgggcga tggggccctc ggtgtcgtat ccggcgagct ccggcacttc ctcggtgttg
     2641 tggacgtacc ggaagtgggc cgcgtccaca ccgttctcgg cgagctcctg ccacggcgcc
     2701 gcgacctggt agatgcgggt gaccatctcg gtgaactggt cgggatcgtt gaactcgggg
     2761 acgatcggga cctcccacat cggctcgacg ccgtcggggt ggtaccaggt catgatgagg
     2821 ccgttgcgct cgatcgtggg gaacgtgcgg agcttgccct tggcgttggt gcgcttgctg
     2881 taggggatga gtgtgttggc gccctgcgcg tcgaagcgcc agccgtggaa cgggcaggcg
     2941 atctcacact tgaggacctt gccgccgtgg cccaggtggg caccgaggtg cgggcagaac
     3001 gcgtcctgga cgtggacgcc gccgtcctcg tcgcgccaga gcacgaggtg cttgccgaag
     3061 tagcggagcg gcttggtctc gccgatggcg acgtcggtcg cccacccaac ctggaaccag
     3121 ccgtagggga cggggaacgg tgaacgcttc atggcagacc tcgcctcgtt gatcgcgtca
     3181 gaacgacgaa aaaactagaa cgtgttctag tatagcgacg tcgcctccac cgtcaatctc
     3241 cggggagcct cacccgcagg cggcagtgat cactgggtag ggattgaccg gacgcccgtc
     3301 cagcggccgg atctccaggt ggaggtgtgg cgcaccaccc ttggcgtcac ccgtgttgcc
     3361 gacgtagccg atgacgtcac cggcggcgac cttgccgccc ttggcggtct tcgacaggtg
     3421 cgccgagtag tactgccagc cgttcgaccc cttcagcgtg gcgaacttgc ccgacacgtt
     3481 ggacgacccg taggtcacct tgccgtcggt cggcgccagg atcggggtcc cggccggtgc
     3541 catcatgtcg atcccctcgt ggaaccgggc accgtcggca cggggaaagc cccagtcgaa
     3601 cgcgtaggtc gagccggcaa ccggacagac cattcccggc gtctgcccgc cgctgatcgt
     3661 tagcacctgg ccgatccgga tctggttcgc cgacttcaac tggttcgcgg cgacgatcgc
     3721 gtcggtcgtc gtgccgtgac ccttggcgat ccgggcgacc gtgtcgcccg cgaccacggt
     3781 gtacgtcgac gtcggaccgg accccgcggg cggcgccatg cgtcctgggt tcggctcgtc
     3841 gagcagcagg cgagccccgg cgtacacggt gtccttggtc atgccgttgg cgctgcggat
     3901 ctgcgcgacc gggatgccat acgtcttcgc gatgcccttc aggccttcgc caggggcgac
     3961 gaggtgatat cgcaggccgc tcaggcgccc gctgtcgacg accttggcgg cagcgctggg
     4021 cgcggcgccg gccggtcggg cgggccacga ggcgagggcg aaggacgtca aggtcatggc
     4081 gaccacaacg aggaggcggt ggcggttccg ggcggacccg aagcggccgg tgggctgggg
     4141 aagcagtgtt gccggcatac cgccccatcg gccccggttc gacggagatg agtcgttcgg
     4201 cctacttcgc cgtgttcact ggctcgatcg gggggcggtc ccccggcaag cgtgcctaca
     4261 ggttgaagac gcgcttcgcg ttctcgcgga ggaacttcgg ccagacctcg tccttgaacc
     4321 cgacgttcgg catctcgctg aagatgcggt cgagcgacag gcccatcggg aagtacccgg
     4381 cgtacatgat cttgtccgcg ccgcgggtgt tggcgaagcg gatgatctcc tcggggtagt
     4441 gcttcggggc gaacgccgag gtggagtagt agaggttcgg ccacttgagc atgagcttca
     4501 cggccaggtc ggtccacgga tcggcgccgt ggcgcatcac gaacttgagc tcggggaaga
     4561 accagcacac ctcgtcgatc agctcgacgt gctggggcat catcgggaca cggggaccgg
     4621 gaacgcccac gcagcagagc acggggatgt cgagctcgca ggccttggcg tacaccgggt
     4681 acatgcgctt gtcgttgatc gggacgccgg ggttgcagcc ggcggggaag agcgtgatcg
     4741 ccttgaggtc gacccgttgg gcggcctcgt tgatcttgtg gatggccttc atgccctcgt
     4801 tggggtcgac ggcgaggccg gcaacgaacc ggtcggggtg ctcgatgcac gcgtcgagtt
     4861 ggatgccgcc ggccaggttg atgccgatga gcgccttctc gatgccgtgc ttgttgagct
     4921 ggtcgatcgt gtactcgacc atgtcgccct cggtcgggtc ctccggcacc tccttgaaca
     4981 tgtactgcgc cgggaacttg aaggtggccg actcggcatc gcggaggttc ggcttcagga
     5041 agtcgtacca cccttccttc ttgccgggat cagggatccc cagcatgagg tcgatcactc
     5101 cgatgtcagt aggcataccc atgcccgaaa tctagaacaa gttctagttg ggtgtcagga
     5161 gtgcccgacc gggagtcgtc aggaggattt cgtcaggagc gcggcacggc tggcgattcc
     5221 cgacactcgg cgggcttcgc ggaggaagtt ccggtcgacg ccgttggtca ggtaacagac
     5281 cgacaggccc gtctcggggt ccgcccacgc gatctgcccg gccgcgccgt tgtgtccgaa
     5341 ggcccgcgcc gagacgttgt gccccatgcc gcggaaggac gacgtgccgt cgtcgccagc
     5401 ggcgatcagg ccgagcgtac ggttcgccgg cgtgcgcagg atcgggtcgg ggagggttgt
     5461 gcggatgtgg ccggtggcgt cggcgaggac gtcggcatcc cacaggccac ccggattgtg
     5521 gaggaacgcc tggtagagca
//
LOCUS       DAOSOA010000221         5526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_14846_length_5526_cov_5.258454, whole genome shotgun sequence.
ACCESSION   DAOSOA010000221 DAOSOA010000000
VERSION     DAOSOA010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14846_length_5526_cov_5.258454"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKY13_12415"
     CDS             complement(<1..210)
                     /locus_tag="PKY13_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT80788.1"
                     /translation="MKMTRRAATAELTTAADGSMMVQLAGELDHDGVVVVEGAVQRAA
                     ATAANTLTLDAERVTFVDSAGLRLVL"
     gene            complement(356..1360)
                     /locus_tag="PKY13_12420"
     CDS             complement(356..1360)
                     /locus_tag="PKY13_12420"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80789.1"
                     /translation="MTDVTGTPVETAALSTAELQGQLEDYRRELTGYCYRMLGSGFEA
                     EDAVQETMVRAWRSLDSFEGRSSLRSWLYRIATNVCLDMLKGRERRARPMDLGPSSTA
                     AGVLGSPEPEHAWVQPIADSRVVPVDGDPAELAASRESIRLAFVAALQHLPPKQRAVL
                     ILREVLRWKADEVAALLDTTVASVNSALQRSRATLDEGRLAASEPVAVAADDAALLDR
                     YVDAFERYDIGELVSLIHEDATFSMPPFALWLQGPSEMAAWYLGQGIACKDSRLLATR
                     ANGTAAFGAYKLAPDGTRRPFALQLIETDGHRIVGLHHFLDPALFAAFGFPDHLEPEE
                     "
     gene            complement(1402..2442)
                     /locus_tag="PKY13_12425"
     CDS             complement(1402..2442)
                     /locus_tag="PKY13_12425"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HOT80790.1"
                     /translation="MTPPPPDRTPPPARLVSGGQFAFGTYDAPVPEVNLLDRWSGPAR
                     AFHAPRLKEWQAFQLMNEHWFVLGAVYDAKLLGLVQIVAVEQRSGTVHRFEHKVPSAR
                     LRVAQGLEHTQSQGRSHGLTVTFTNSLRSGRLRVTAQQEARRGVAPMTLDVAGSCAPG
                     EAGHLVICHPFPDGTPLYSNKCMMPADGALTLGEEHVEFADGDAVLILDDHKGHYPSP
                     MKYDWVTAAGRDDTGRVVGFNLTDNQVRDPDIYNENALWVGDEVHRLGAVTFERPKGV
                     HGPWRVRDRLGQVDVTFEPTVRNEQHVGLRSFLVDYYGPFGRYSGEIDTGAARLTVDN
                     MFGMGEQKLIRV"
     gene            2534..2980
                     /locus_tag="PKY13_12430"
     CDS             2534..2980
                     /locus_tag="PKY13_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT80791.1"
                     /translation="MSAAPRHNVCSHIGLCVSDLDRSLRFYCDGIGFEVAEGYDLDDT
                     MLPGLDLALEVPGPVVVRSQMILLGDFRIELLAYRSPAVDGSPSVRRNQVGLTHLSFL
                     VDDIDAAIARAVEHGGHLLEPTRQSVGIELVFLADPDGTRVEFMQR"
     gene            2977..5289
                     /locus_tag="PKY13_12435"
     CDS             2977..5289
                     /locus_tag="PKY13_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HOT80792.1"
                     /translation="MTDGAASRTLPHLAHWGAFRVTVEDDRVIAVAGHPDDPAPSPLH
                     ENVVEGAERGRVARPAIREGWLRNGPGPDDQRGTDRYVEVPWDEALDLAATELDRVRT
                     EHGNEAIFGGSYGWSSAGRFHHAQSQLHRFLNCIGGYTASVNSYSLAASEVLVPHVLG
                     TLDDIRYRATTWPDILEHTELVVAFGGMNVKNAWVTPGGVTRHTLAPALAEAGRRGLA
                     FELVGPLRDDLPADVRARWHQVRPGTDTAVMLALIHQLVVTDQYDRGFLERCTVGWER
                     IVDDVLGRRDGVVKDAAWAESISGVPAATIVELADRLVSQRSLITVSWALQRAEHGEQ
                     PVWAAIALAAFVGQIGLPGAGFGHGYGSMADVGLPTVPGALPTLRQGRNPVRTFIPVA
                     RITDLLTRPGEELDYNGRRLRLPDTRLVYWAGGNPFHHHQDLFGLRRAFARPDTVIVN
                     EPYWTATARHADIVFPITTTLERDDIGAGRNDGYVSAMPRVLPPFGDARDDYAVFCGL
                     AARLGVEETFSEGHDTMGWLAHLYGRFRARHGEAGVDLPEFGEFWERGEVTLPATPRH
                     HTVFEGFRHDPDTHPLPTPSGRIELFSSTVDSFGYDDCPGQPTWLPPREWAGSEVADR
                     HPLTLVANQPARRLHSQLDGGRYSQAGKIGGREVVRLHPADAVARGIGEADVVRLFND
                     RGACLAAAELTDSVAPGVVQLSTGAWFTPVDDPTGPLCVQGNPNVLTADHGTSRLAQG
                     SIGQLTMVEAERYDGVPDDRDPYLPPIVGV"
     gene            5289..>5526
                     /locus_tag="PKY13_12440"
     CDS             5289..>5526
                     /locus_tag="PKY13_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80793.1"
                     /translation="MAADRPTLVALPSLDDLYAAEPEEFVAARNALAKQLKAAGDKDT
                     AAEVARLRRPPVSAWALNQVARSRPDEIEALMTAA"
BASE COUNT          815 a         2015 c         1895 g          801 t
ORIGIN      
        1 gaggaccagc cggaggcccg cggaatcgac gaaggtgacc cgctcggcgt cgagggtcag
       61 cgtgttcgca gcggtggcgg cggctcgctg caccgcgccc tcgaccacga ccacaccgtc
      121 gtggtcgagc tcaccggcca gctgcaccat catcgaaccg tcggccgccg tcgtcagctc
      181 cgcggtggcg gctcgacgcg tcatcttcat gggtccaatc ctcacggtca ccttcgttcg
      241 ctggccgtcc gtcagccggg catcgtcggt ccgggcccga aacgctgttc ggccgttgat
      301 acccggtgtg gcccggtcca aacatgacga agcggtcacc cgccgacgcc ggcgactact
      361 cctcgggttc gaggtgatcg gggaacccga acgccgcgaa cagggccggg tcgaggaagt
      421 ggtggaggcc gacgatgcgg tggccgtcgg tctcgatgag ttggagggcg aacggccggc
      481 gggtgccgtc gggagcgagc ttgtaggcgc cgaacgcggc ggtgccgttg gccctcgttg
      541 cgagcaggcg ggagtccttg cacgcgatgc cctggcccag ataccacgcg gccatctccg
      601 acgggccctg caaccacaag gcgaacggcg gcatggagaa cgttgcgtcc tcgtggatga
      661 gcgagaccag ctcgccgatg tcgtagcgct cgaacgcatc gacgtagcga tccagcagcg
      721 cagcgtcgtc ggcggcgacc gccacgggct ccgacgccgc gagccggccc tcgtcgaggg
      781 tggcacgact ccgctgcagc gcgctgttga ccgacgccac ggtggtgtcg agcaatgccg
      841 cgacctcgtc ggccttccac cggagcacct cgcgcaggat caacaccgcc cgctgcttgg
      901 gaggcaggtg ctggagcgct gccacgaagg ccaggcggat gctctcgcgt gacgccgcga
      961 gctcggcggg atcgccatcg acggggacca cacgggaatc ggcgatcggt tgcacccacg
     1021 cgtgctcggg ctcgggcgaa ccgagcaccc ccgctgcggt ggagctcggc ccgaggtcca
     1081 tcggacgcgc ccgccgctcg cgccccttca gcatgtcgag gcacacattg gtggcgatgc
     1141 ggtagagcca cgaccgcagc gaggaccgac cctcgaacga gtcgagcgac cgccacgccc
     1201 ggaccatggt ctcctggacg gcgtcctcgg cctcgaagcc ggacccgagc atgcgatagc
     1261 agtacccggt gagctcacgg cggtagtcct cgagctggcc ctggagttcg gcggtactga
     1321 gcgctgccgt ctcgacgggg gtgccggtca cgtcggtcat ggccacatcg tagggaccac
     1381 caccgacagc gaccgctcgg ttcagacccg aatcagcttc tgttccccca taccgaacat
     1441 gttgtcgacg gtgagccggg ccgcacccgt gtcgatctcg ccggagtagc ggccgaacgg
     1501 gccgtagtaa tcgacgagga aactgcggag accgacgtgc tgctcgttgc ggaccgtcgg
     1561 ctcgaacgtc acgtcgacct gaccgagccg gtcacggacg cgccacggac cgtgcacccc
     1621 cttgggtcgc tcgaacgtca cggctccgag acggtggacc tcgtcgccga cccacagcgc
     1681 gttctcgttg tagatgtcgg ggtcgcgcac ctggttgtcg gtgaggttga acccgaccac
     1741 ccgaccggtg tcgtcgcggc cggcggccgt cacccagtcg tacttcatcg gcgagggata
     1801 gtgccccttg tggtcgtcga ggatgagcac ggcatcgccg tcggcgaact cgacgtgctc
     1861 ctcgccgagg gtcagcgcac cgtcggcggg catcatgcac ttgttcgagt agagcggggt
     1921 gccgtcggga aacgggtggc agatcacgag gtgcccggcc tcgccgggtg cgcacgaacc
     1981 ggcgacgtcg agcgtcatcg gcgcgacccc tcgacgggcc tcctgctgtg ccgtgacccg
     2041 gagccgcccc gatcgcagtg agttggtgaa ggtgaccgtg agtccgtgcg atcgaccctg
     2101 cgactgggtg tgctccaacc cctgggcgac gcggaggcgc gccgacggca ccttgtgctc
     2161 gaagcggtgg acggtcccgg accgctgctc gacggccacg atctggacca gaccgaggag
     2221 cttggcgtcg tagaccgcgc cgagcacgaa ccagtgctcg ttcatcagct ggaacgcctg
     2281 ccactccttc aaccgcgggg cgtggaacgc ccgtgccggc ccggaccatc ggtcgagcag
     2341 gttcacctcc ggcaccgggg cgtcgtaggt gccgaacgcg aactgcccgc ccgagaccag
     2401 tcgagccggg ggcggcgtgc ggtccggggg cggcggcgtc atggtcggcg aggctatccc
     2461 gcacccgaaa tcgggcacga tccgatcgtc gaacgacctc ccgttcgctc gcgatctgtt
     2521 ggagaatggc gccatgtccg ctgcacctcg ccacaacgtc tgctcccaca tcggcctctg
     2581 cgtgagcgac ctcgaccgat ccctgcgctt ctactgcgac ggcatcgggt tcgaggtggc
     2641 cgagggctac gacctcgacg acacgatgct gcccggtctc gacctcgcgc tcgaggtgcc
     2701 cggccccgtc gtcgtgcgca gtcagatgat cctgctcgga gacttccgca tcgagctcct
     2761 cgcctaccgc tcccccgctg tcgacgggag cccgtcggtc cgccgcaacc aggtcggcct
     2821 cacgcacctg tcgttcctcg tcgacgacat cgacgccgcc atcgcccgcg ccgtcgagca
     2881 cggtggtcac ctgctcgagc cgacgcgtca gtcggtcggc atcgagctcg tgttcctcgc
     2941 cgaccccgac ggcacccgcg tggagttcat gcaacgatga ccgacggcgc cgcgagccga
     3001 acgctcccgc acctcgccca ctggggagcg ttccgggtga ccgtcgagga cgaccgcgtc
     3061 atcgccgtcg ccggccaccc cgacgacccg gcgccgtcgc cgctccacga gaacgtggtg
     3121 gagggcgcgg agcgaggccg cgtggcgcgt ccggcgatcc gcgagggctg gcttcgcaac
     3181 ggccccggcc ccgacgacca acgcggcacc gaccgctacg tcgaggtccc gtgggacgag
     3241 gcactcgatc tcgccgccac cgaactcgac cgggtgcgga ccgaacacgg caacgaggcg
     3301 atcttcggag ggtcctacgg atggtcgagc gctggccggt tccaccacgc gcagagccag
     3361 ctccaccggt tcctcaactg catcggcggc tacacggcgt cggtcaacag ctactcgctg
     3421 gcggcctcgg aggtcctcgt cccccatgtg ctgggcacgc tcgacgacat ccgataccgg
     3481 gccacgacct ggcccgacat cctcgagcac accgagctgg tggtggcgtt cggcggcatg
     3541 aacgtcaaga acgcctgggt gacccccggt ggggtgaccc gccacaccct cgctcccgcc
     3601 ctcgccgagg cgggccggcg aggtctggcg ttcgagctcg tcggcccgct ccgcgacgac
     3661 ctgcccgccg acgtgcgcgc ccggtggcac caggtccgcc ccggcaccga caccgcagtg
     3721 atgttggccc tcatccacca gctcgtcgtc accgaccagt acgaccgcgg gttcctcgaa
     3781 cggtgcacgg tcggttggga acgcatcgtc gacgacgtgc tcggccgacg cgacggtgtc
     3841 gtgaaggacg cggcgtgggc cgaatcgatc tcgggcgtgc ccgccgccac catcgtcgag
     3901 ctggccgacc gcctggtgtc ccagcggtcg ctcatcacgg tgagctgggc gctgcagcgc
     3961 gccgagcacg gcgaacagcc ggtgtgggca gcgatcgcgc tcgccgcctt cgtcggccag
     4021 atcggcctcc cgggcgcggg gttcggtcac ggctacggct cgatggccga cgtcggtctg
     4081 cccaccgtgc cgggggcgct gccgaccctg cgccagggcc gcaacccggt gcggacgttc
     4141 atcccggtcg cccgcatcac cgacctgctg acccggccgg gcgaagagct cgactacaac
     4201 gggcgtcgcc tccgcctgcc cgacacccgg ctcgtgtact gggccggtgg caacccgttc
     4261 caccaccacc aggacctgtt cgggctgcgc cgagcgttcg cccggcccga cacggtgatc
     4321 gtcaacgagc cgtactggac ggcgacggcc cgccacgccg acatcgtgtt cccgatcacg
     4381 acgacgctcg agcgcgacga catcggcgcc ggccgcaacg acggctacgt gagcgccatg
     4441 ccccgggtgc tccctccgtt cggcgacgcc cgcgacgact acgcggtgtt ctgtggcctc
     4501 gcggcacgcc tcggcgtcga ggagacgttc tcggagggtc acgacacgat ggggtggctg
     4561 gcgcatctct acggccgctt ccgcgcccgt cacggcgagg ccggcgtcga tctcccggag
     4621 ttcggggagt tctgggagcg cggcgaggtc accctgcccg caacaccacg gcaccacacg
     4681 gtcttcgagg gcttccggca cgatcccgac acccatccgc tccccacccc gagcggacgg
     4741 atcgagctgt tctcgtcgac ggtggactcg ttcggctacg acgactgccc gggccagccg
     4801 acctggctgc caccccggga gtgggccggc tcggaggttg ccgatcgcca cccgctcacg
     4861 ctggtcgcca accaaccggc ccggcggctg cacagccagc tcgacggcgg tcggtacagc
     4921 caggccggca agatcggcgg tcgggaggtc gtccggctcc atcccgccga cgccgtcgcg
     4981 aggggcatcg gcgaggccga cgtcgtccgc ctgttcaacg accggggcgc ctgcctggcg
     5041 gctgccgagc tcaccgactc ggtcgcgccc ggtgtcgtgc agctctcgac gggggcgtgg
     5101 ttcaccccgg tcgacgaccc gactggtccg ctgtgcgtcc agggcaaccc caacgtgctc
     5161 accgcggacc acggcacgtc gcgcctggcg caaggttcca tcgggcagct gaccatggtc
     5221 gaagcggagc ggtacgacgg cgttcccgac gaccgggacc cgtatctccc accgatcgtg
     5281 ggggtgtgat ggccgcggat cgcccgacgc tcgtcgcgct gccgtcgctc gacgacctct
     5341 acgccgccga gcccgaggag ttcgtcgccg cccgcaacgc gttggccaag cagctcaagg
     5401 cagcgggcga caaggacacg gcggccgagg tcgcgaggtt gcggcgcccc ccggtgtcgg
     5461 cgtgggccct caaccaggtt gcccgcagcc gtcccgacga gatcgaggcc ctcatgaccg
     5521 ccgccg
//
LOCUS       DAOSOA010000222         5449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15096_length_5449_cov_5.652021, whole genome shotgun sequence.
ACCESSION   DAOSOA010000222 DAOSOA010000000
VERSION     DAOSOA010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15096_length_5449_cov_5.652021"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PKY13_12445"
     CDS             complement(<1..836)
                     /locus_tag="PKY13_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80794.1"
                     /translation="MAKRARSARERGLAAWAGYGLGQRLSSLGLALAVVVALVVVVTG
                     GGEGGLNLVVTDEQLAASDGSGVTAAPRRDGDIGASSVDDTAAPATAAAATAADPTFV
                     DDTATVGQAEAGGPQVLGITTVPGRSNRAAKSAAAAKAAKTTADKAATNKPNSSALAA
                     FFGAALRGSKTDSPTSSPSTLSAPSSPSAPSSPSSPAPTPAPASPTTRPAATNPPTTR
                     PPATTPPTESPPATTPPTTRPPVTAPPTTRPPVTVPPTTEPPVTVPPTTEPPVTSPPT
                     TAA"
     gene            983..2218
                     /locus_tag="PKY13_12450"
     CDS             983..2218
                     /locus_tag="PKY13_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT80795.1"
                     /translation="MATRHPPADHAQRATDDRSQRATVAYLAFLGIVMAVGVDIALPA
                     FARISDTFSLSDGDGQVSLVGTTYFLGMATGQLVFGPASDRFGRRRCLQIGIVLAAIG
                     ALGAAIAPSFTLLLVARGVWGLGAAAPAVLRTAIARDLFDGDEMARVVTVIMAVFLIG
                     PIFVPSFGQLILASGSWRAVFVAALVIAAVGFVWTLAFGETLRPEDRRPLAAGPLLEA
                     LGAVVRTRATVGYILAQTFATGAFVIFLGSGQPIIDRIYGRGSQFALFFGIAGTVMSL
                     ALVGNNRLIRMHGARTMALVAAISFVTLATGGLVMMLLTDGRPPFAAWFVWLALSNSL
                     PTLMSPMCNSLALEPMGRLAGTASAILGFVTLAGGALLAQVFDSMIDRSVTPMAVGYV
                     VYGSLALAALLWARSRPGT"
     gene            complement(2201..3253)
                     /locus_tag="PKY13_12455"
     CDS             complement(2201..3253)
                     /locus_tag="PKY13_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOT80796.1"
                     /translation="MINWITASPRRDRDGLLMVVDGGATHSRVVIADTTGELLGFATG
                     QPTNARSVGVEQAAANLADTLHRAIVEGSGDPDDVVYGLVTSAAVDTDEQSENLSAAA
                     RSLLPNATVFSVPDTMGCWAATNRLGPAVAVISGTGSVVVAADRARGLWRRFGGWDFL
                     LGDEGSGYSLGRAALQETLLVNEGRSGAEALSAAVLASKPAAAKHVTDAEDLPDAVHT
                     PAIDKAWIASFAPLVLGLADSGDEHCLAIVRREIRILAGAAAAGIDALGVEGGASTPV
                     GLFGGTFNSPSLHAAFVDAVCELTARPFDAAVPAHTALVGAYAMALGADPSVSAAEID
                     AASDRLASSLSSRPGT"
     gene            3290..4132
                     /locus_tag="PKY13_12460"
     CDS             3290..4132
                     /locus_tag="PKY13_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT80797.1"
                     /translation="MQTQPPEPGAPQYVAIGHVCVDELVDAPPRLGGTVFFSSAQAAA
                     MGCRVTVVTSCTPDVATDAAAALPAGTALQVHDTGRDTRFGFGPDAADGPQRLLSVAA
                     PIDSFATEGRPDILHLAPVFNELTPNLVATARERARFVGATPQGLLRGTDADGGLVFL
                     ADNWPERHDFADAIVLNDEEYAHLHGIGLLDRFDGLVFRTLGPAGAALVQHGDEIARH
                     ELDPDERPVPPRRTIGAGDVFAAAAFVSLARGDSPADALRIAVDSATGYVRRPTDAPT
                     FVAS"
     gene            complement(4185..4670)
                     /locus_tag="PKY13_12465"
     CDS             complement(4185..4670)
                     /locus_tag="PKY13_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80798.1"
                     /translation="MARPALHRLTTAWVASLLLLAPACSDDEVVDRTPASTATSPDDA
                     AFDQPGSHDASVSVHFTIIGDGSGPDPSVDALAELEDRIVDAIEPTGIGVLEGDEFAE
                     GTVTLSIYGPDLDRLWAKVRPILETYFARPAYAELRDGGPDQPAKRVDLGPVPTSTTS
                     P"
     gene            complement(4874..5374)
                     /locus_tag="PKY13_12470"
     CDS             complement(4874..5374)
                     /locus_tag="PKY13_12470"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT80799.1"
                     /translation="MCELTTLINDPNLPAGTRLIVRREPLHPGAQRSLFPSLDYRYWG
                     FYTDQTGDPTECDVTMRAHAHVEQHIQRLKESGLCRFPFQNFDANQAWLHTVALAADL
                     VRWFQLVCLPDTWTDVRPKKLRWALFGAPGRLVRTARQTIVRVIDNWPGADMIVGAYQ
                     RITLIT"
BASE COUNT          738 a         1861 c         1975 g          875 t
ORIGIN      
        1 gcagccgtcg tcggcggcga tgtcaccggc ggctccgtcg tgggaggaac cgtgaccggt
       61 ggctccgtcg tgggaggaac cgtgaccggg gggcgcgtcg tcggaggtgc ggtgacgggc
      121 ggacgcgtcg tgggcggcgt cgtcgccggc ggactttcgg tcggcggtgt cgtcgccgga
      181 ggacgtgtgg ttggtgggtt cgtcgcagcg ggacgcgtgg tcggcgacgc cggggcgggc
      241 gtcggcgccg gagacgaggg cgaactcggg gccgacggcg aactcggggc ggacagggtc
      301 gacggcgacg aggtcggcga atccgtcttg gagcctcgca gcgcggcgcc gaagaacgcc
      361 gccagtgcgg agctgttcgg cttgttcgtc gccgctttgt cggcggtcgt cttggccgct
      421 ttcgccgccg cagcggactt ggccgcacgg ttggatcggc ccggcaccgt ggtgatgccg
      481 aggacctgcg gcccgccggc ctcggcttgg ccgaccgtcg cggtgtcgtc gacgaacgtc
      541 ggatcggccg ccgtggcggc cgccgccgtc gcgggtgctg ccgtgtcgtc gaccgacgac
      601 gctccgatgt cgccgtcacg acgaggagct gcggtcacgc ccgaaccgtc ggacgcggcg
      661 agctgttcgt cggtgacgac gaggttgagg ccaccctcgc cgccgcccgt gacgacgaca
      721 accagtgcca cgaccaccgc caacgccaga ccgaggctgc tcaatcgctg gcccaggccg
      781 tagccggccc acgcggcgag gccgcgttct ctcgccgagc gcgcccgctt ggccacgtcc
      841 gccggaaggt atcggaggtc atcgagtcga cgcctcaccg tatgactgtt ccccaggttc
      901 agtttcatgt catatccgcc tcccccagac attagatcga acgggcgggc aggtgtgcga
      961 ccttgggtag gttggccccg ccatggccac acgacatccc cctgccgatc atgcgcagcg
     1021 ggccaccgac gatcgctcac agcgggccac cgtcgcctac ctggccttcc tcggcatcgt
     1081 catggcggtc ggcgtcgaca tcgcacttcc ggcgttcgcc cggatcagcg acacgttctc
     1141 gctgtcggac ggggacggac aggtgtcgtt ggtcggaacg acgtacttcc tcggcatggc
     1201 gaccggtcag ctcgtgttcg gccccgcgtc ggaccggttc ggtcggcgcc ggtgcctaca
     1261 gatcgggatc gtgctcgcag cgatcggcgc actcggcgcc gcgatcgctc cgtcgttcac
     1321 gttgctgctg gtggcccgag gtgtgtgggg cctcggggcg gcggcgccgg cggtgctgcg
     1381 gacggcgatc gcccgcgacc tcttcgacgg cgacgagatg gcgcgggtcg tgaccgtcat
     1441 catggcggtg ttcctcatcg gcccgatctt cgtcccgtcg ttcggccagt tgatcctcgc
     1501 gtccggctcg tggcgtgccg tgttcgtcgc cgcgctcgtc atcgccgccg tcgggttcgt
     1561 gtggaccctc gcgtttggcg agaccctccg gcccgaggac cgccgacctc tcgccgccgg
     1621 tccgctgctc gaggcgctcg gagccgtcgt gcgcacccga gcgaccgtcg gttacatcct
     1681 cgcccagacg ttcgcgaccg gagcgttcgt gatcttcctc gggagcggtc aaccgatcat
     1741 cgaccggatc tacggtcggg gatcgcagtt cgccctgttc ttcgggatcg ccggcaccgt
     1801 gatgtcgttg gcgctcgtgg gcaacaaccg gctgattcgg atgcatggcg cccgcaccat
     1861 ggccctggtc gccgccatct cgttcgtgac cctcgctacc ggagggctcg tgatgatgtt
     1921 gctcaccgac ggccgcccgc cgttcgcggc ctggttcgtc tggctcgcgc tgtccaattc
     1981 gctgccgacg ctcatgtcgc cgatgtgcaa ctcgctcgcg ctcgagccga tgggccgcct
     2041 ggcgggaacg gcgtcggcga tcctcgggtt cgtcacgctc gccggcggag cgctcctcgc
     2101 tcaggtgttc gactcgatga tcgaccggtc ggtgaccccg atggccgtgg gatacgtggt
     2161 gtacggatcg ctggcgctcg ccgcgctgct gtgggcgcgt tcacgtcccg ggacgtgacg
     2221 acagcgacga tgcaaggcga tcggacgcgg cgtcgatctc cgccgcgctg accgacgggt
     2281 cggcacccaa cgccatggcg tacgcaccga cgagcgccgt gtgagccggc acggccgcgt
     2341 cgaacggacg ggcggtgagc tcgcacaccg cgtcgacgaa ggcagcgtgc agcgacggcg
     2401 agttgaacgt tccgccgaag agaccgacag gggtcgaggc accgccctcg accccgagcg
     2461 cgtcgattcc cgccgcggcg gcgccggcca ggatgcggat ctcacgacgg acgatcgcca
     2521 ggcagtgctc gtcgccggag tcggcgaggc ccaacaccaa cggagcgaac gaggcgatcc
     2581 acgccttgtc gatcgcagga gtgtggaccg cgtcgggtag gtcttcggcg tcggtcacgt
     2641 gcttcgctgc cgcaggcttg ctggcgagca cggccgcgct caacgcttcg gcgcccgacc
     2701 ggccctcgtt cacgagcagg gtctcctgca gcgccgcccg accgagcgag tagcccgagc
     2761 cctcgtcgcc gaggaggaag tcccatccac cgaaccgccg ccacaggccc cgcgcccggt
     2821 cggcggccac aacgacgctg ccggtaccgg agatgacggc gacggccggc ccgagccggt
     2881 tggtcgcagc ccagcacccc atcgtgtcgg gcacggagaa cacggtcgcg ttcgggagga
     2941 gcgaccgagc ggccgccgac aggttctcgg actgctcgtc ggtatcgacc gccgcgctcg
     3001 tgacgaggcc gtagacgacg tcgtcgggat cgccggagcc ctcgacgatc gcccggtgca
     3061 gggtgtcggc caggttcgct gccgcctgct cgacaccgac cgatcgcgcg ttggtcggct
     3121 ggcccgtcgc gaacccgagg agctcgccgg tggtgtcggc gatgaccaca cgggagtggg
     3181 tcgctccccc gtcgacgacc atcaggaggc cgtcgcggtc gcggcgaggg gaggcggtga
     3241 tccagttgat catgagcgtc gaccctatcg accggtactg tcgcccaccg tgcagaccca
     3301 acctcccgag ccgggcgctc cgcagtacgt cgcgatcgga cacgtctgtg tggacgaatt
     3361 ggtcgacgca ccgccgcgtc tcggcggcac cgtcttcttc tcctccgcgc aggcggcggc
     3421 gatggggtgc cgggtcaccg tcgtcacgtc gtgcacgccc gatgtcgcga ccgacgcggc
     3481 ggcagcgctc ccggcgggca ccgcgctgca ggtccacgac accgggcggg acacgcggtt
     3541 cggcttcgga cccgacgccg ccgacggacc gcaacgcctg ctgtcggtcg ctgcaccgat
     3601 cgactcgttc gcgaccgagg gccggcccga catcctgcac ctcgccccgg tcttcaacga
     3661 gctcaccccg aacctggtcg caacggcccg ggagcgggct cggttcgtgg gcgccacgcc
     3721 acaaggcctg ttgcgcggta ccgacgcgga cggcggcctc gtgttcctcg cggacaactg
     3781 gccggagcga cacgacttcg ccgacgccat cgtgctcaac gacgaggagt acgcacatct
     3841 ccacggcatc ggcctgctcg accggttcga cggcctggtc ttccggaccc tcggaccggc
     3901 gggcgccgcc ctggtgcagc acggcgacga gatcgcccgg cacgaactcg accccgacga
     3961 acgacccgtc ccgccccgcc gcacgatcgg tgccggcgac gtgttcgccg ccgctgcgtt
     4021 cgtgtcgctc gcccgcggcg actcccccgc cgatgcgttg cgcatcgctg tcgactcggc
     4081 gacgggctac gtccggcggc ccaccgacgc accgacgttc gtggccagtt gaagtggtgc
     4141 gtgcgcgcga tggaaacgtc gcgccagcgc accacttcga aagatcaggg cgaggtcgtg
     4201 ctcgtcggca cgggaccgag gtcgacgcgc ttcgccggct ggtcgggccc gccgtcgcgg
     4261 agttccgcgt acgccgggcg ggcgaagtag gtctcgagga tcggccgaac cttcgcccac
     4321 agacgatcga ggtcgggccc gtagatcgag agcgtcaccg tgccctcggc gaactcgtcg
     4381 ccctcgagca ccccgatgcc ggtgggctcg atggcgtcga cgatgcggtc ctcgagctcg
     4441 gccagcgcgt ccaccgacgg gtccggcccc gacccgtccc cgatgatcgt gaagtggacc
     4501 gagaccgacg cgtcgtggga accgggctgg tcgaacgccg cgtcgtccgg cgacgtcgcg
     4561 gtcgaggccg gggtgcggtc gaccacctcg tcgtccgaac aagcgggagc gagcagcaac
     4621 aacgatgcga cccacgcggt cgtcagacga tggagagctg gccgcgccac ggcagatgtc
     4681 tagccccaac tcttcacgcg gcgatttggg atgagtcgcg acgctgattt cggtgtcggg
     4741 tcgggtgcgt tcggctgatt cggttcgttg cccggggtgg ctgtctggtc gaggggaccg
     4801 aactgggggt tcggactggt ggtggggcgc gcgtggtgag cggggtgccg gtcgtgttcg
     4861 ggttcagggc tgttcaggtg atgagcgtga tgcgttggta ggcgccgacg atcatgtcgg
     4921 ctccgggcca gttgtcgatg acccggacga tggtctgccg tgcggtgcgc acgagccggc
     4981 cgggcgcccc gaacagggcc cagcgaagct tcttgggtcg cacatcagtc cacgtgtcgg
     5041 ggaggcacac gagttggaac cagcgaacca ggtcagccgc gagggcgacg gtgtggagcc
     5101 acgcctggtt ggcgtcgaag ttctggaacg ggaaccggca caggcctgac tctttcaggc
     5161 gttggatgtg ttgttcgacg tgtgcgtgag cgcgcatcgt gacgtcgcac tcggtggggt
     5221 caccggtctg gtcggtgtag aacccccagt acctgtagtc cagcgacggg aacagggagc
     5281 gttgagctcc ggggtgtaac ggttcgcgtc gcacgatcag ccgtgtccct gccgggaggt
     5341 tcgggtcgtt gatgagggtg gtgagttcgc acacggcagc accgtcggtg cgtggccggc
     5401 cagcgcggtc caatgctggg gtccaggcag ggcagcacgg actcaatgt
//
LOCUS       DAOSOA010000223         5428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15179_length_5428_cov_6.391960, whole genome shotgun sequence.
ACCESSION   DAOSOA010000223 DAOSOA010000000
VERSION     DAOSOA010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15179_length_5428_cov_6.391960"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..549)
                     /locus_tag="PKY13_12475"
     CDS             complement(67..549)
                     /locus_tag="PKY13_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80800.1"
                     /translation="MNQVAPVAAPERDRLRSGVLVVVVVATVGIVVELATSRHWHGGI
                     QLIPWFAAGLLLVATALIGRGAPGGVRVGRVLAAVVSLASLFGVWEHVEGNYDSGPLD
                     FRYATSWASMSEATRWWKAVSGGVGAAPPIVPGALALVAGLLVVATFGPGGRSGDRTG
                     "
     gene            complement(546..977)
                     /locus_tag="PKY13_12480"
     CDS             complement(546..977)
                     /locus_tag="PKY13_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80801.1"
                     /translation="MQPDTARHRLRSLAVVALALPALTVGCGDDTGTAAIATTAAAAA
                     STTVGGSDTTSPDTTSPDTAADTTDASGATTKVNANDASVEELAAAFEAAGIPNAARW
                     AHEVEEYRPYSDDGWAHLRQELSKYNIDDATFEQIVATLEL"
     gene            1073..1720
                     /locus_tag="PKY13_12485"
     CDS             1073..1720
                     /locus_tag="PKY13_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT80802.1"
                     /translation="MTIRVFLLDDHEVVRRGVADLLGMEDDIEVVGEAGTAEQAISRI
                     LATAPDVAILDVQLPDGSGVEVCREVRSVRPELVCLMLTSFAEDEALFEAIMAGAAGF
                     VLKQVKGSDLIDSVRKVARGESLLDPRTTQRVLERIRRAPEEDERLKGLTPQEKRILS
                     LLAEGRTNRQIADEMFLAEKTVKNYVSNLLSKMGMSRRTEAAVYAARLAERRGTT"
     gene            1805..2458
                     /locus_tag="PKY13_12490"
     CDS             1805..2458
                     /locus_tag="PKY13_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT80803.1"
                     /translation="MIRVFLLDDHEIVRRGVRELIDGEDDLVVVGEAGTGAEAIARLP
                     STRPDVAVLDVQLGEGSGVEVCRDIRSMMPEVGCLMLTSFSDDEAVVESVLAGAQGYV
                     LKQVVGSDLIESIRAVAAGRSLIPADVVEKVRDHVRAIQAVEDRIAELGPRERAILAL
                     LADGHTNREIGERLFLSEKTVKNYMSNVLRTLGMHRRTEAAVFAAKAAQRREKDLRL"
     gene            2518..2970
                     /locus_tag="PKY13_12495"
     CDS             2518..2970
                     /locus_tag="PKY13_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT80804.1"
                     /translation="MDPNTEDRDLVELDEDECATLLALESVGRLAVGDATAAPDVVPV
                     NFVLRDGDPIFRSHPGVLLDRVLSSPVSLQVDRFDWFHRTGWSVLVHGVAELIDPDIA
                     GALIEEGDDTLDTWAPGDQPVLVRVRAEYLSGRRIELQQIPLDGRGYL"
     gene            3009..3419
                     /locus_tag="PKY13_12500"
     CDS             3009..3419
                     /locus_tag="PKY13_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT80805.1"
                     /translation="MPIDGSDEHGLGLIDEATCVRLLSHGHIGRLGLSVGSLPVVLPV
                     NYLLQDHTIVFRSEDGDKTRAAELGSVACLEIDHFDLFEHSGWSVLATGRLSIAPNDR
                     AAVYDRLPVVPWALQQSSRFVELSVELVSGRSIR"
     gene            3416..3844
                     /locus_tag="PKY13_12505"
     CDS             3416..3844
                     /locus_tag="PKY13_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT80806.1"
                     /translation="MTELQLDHAGLEILNPAECRELLASTAVGRIAFIDRGDPVILPV
                     TIGMWEGAVVFTTAPGSKLEAAIMNRPVAIEVDDWDAERHVGWSVLVKGMAMIVDDGR
                     EIDSLDRLSVKSWSRPEIPKAWVRIVANEITGRRVVPISR"
     gene            3936..4793
                     /locus_tag="PKY13_12510"
     CDS             3936..4793
                     /locus_tag="PKY13_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80807.1"
                     /translation="MTVTSGPTHGSHSTMPQAPVVPGPEARARQHGHGEQNGRADLHE
                     LVESLAERTLRPSDDSQAASRILVAANTAAEQLLSDARAEATALVDNAKARTAELLTV
                     AQELADRELHAERTRVSGAVAAWNAQQREIAARLDALRTSLATYQADAEATLAAIADA
                     LAGLAAVEIDAGAARATTTVGRDLDAEPVPETAAPADLPAPAEAEGRVIATPLPAAST
                     PAGPIPAGPATPAEEHDGPHAHVPAQARDQADTSEVGAQRTSLFLSPAATAASSSQTA
                     TTRQGLFGR"
BASE COUNT          848 a         1865 c         1893 g          822 t
ORIGIN      
        1 ccaggccgag aaccggatgc acacgatcaa agcggtgatg gtcgcgaccc tgggccactg
       61 acccggtcaa ccggtccggt cgccggacct tccgccgggg ccgaacgtgg cgaccaccag
      121 gagcccggcc accagcgcga gcgcgcccgg gacgatcggc ggggcagccc cgacgccgcc
      181 gctcactgcc ttccaccaac gcgtcgcctc cgacatcgac gcccaactcg tggcgtaacg
      241 gaagtcgagc ggcccgctgt cgtagttgcc ttcgacgtgc tcccacacgc cgaacaggct
      301 ggccagcgag acaaccgcag ccaggacgcg cccgacgcgc acaccgccgg gcgcgccccg
      361 gccgatcagc gcggtcgcga ccagcaggag gccggcagcg aaccacggga tgagctggat
      421 gcccccatgc cagtggcgac tggtggcgag ctcgacgacg atgcccaccg tcgcgacgac
      481 caccacgacg agcaccccgg aacggagccg gtcacgctcg ggcgccgcaa cgggcgctac
      541 ctggttcaca gctcgagggt cgcgacgatc tgctcgaacg tcgcgtcgtc gatgttgtac
      601 ttggagagct cctggcggag gtgcgcccat ccgtcgtcgc tgtaggggcg gtactcctcg
      661 acctcgtggg cccaccgggc ggcgttgggg atgccggcgg cctcgaaggc cgccgcgagt
      721 tcctcgaccg acgcgtcgtt ggcgttcacc ttggtcgtcg caccggacgc gtcggtcgta
      781 tcggccgccg tgtcgggaga ggtcgtgtcg ggagaggtcg tgtcggaccc gccgacggtg
      841 gtgctggccg cagcggcggc cgtcgtggcg atcgcggcgg tgccggtgtc gtcgccgcac
      901 cccaccgtga gggcggggag ggcaagggcg acgacggcga gcgaacggag ccggtggcgg
      961 gcggtgtcgg gttgcatgaa ccgaaccgta ggaccgtcgc gaaccgcggt cacggaacgc
     1021 gccgcagttc ttacgaactc cttgcagaag agcgggcggg catacttggc gcatgacgat
     1081 ccgcgtgttt ctgctcgatg accacgaggt cgtacggcgc ggggtcgccg atctcctggg
     1141 tatggaggac gacatcgagg tcgtcggcga ggcgggcacc gccgagcagg cgatcagtcg
     1201 gatcctcgcc acggcgcccg acgtcgcaat cctcgacgtg cagctgcccg acgggagcgg
     1261 cgtcgaggtg tgccgtgaag tccgctccgt ccgtcccgaa ctggtgtgcc tcatgctgac
     1321 ctcgttcgcc gaggacgaag cgctcttcga ggcgatcatg gcgggcgccg ccggcttcgt
     1381 gctcaagcag gtgaagggga gcgacctcat cgactcggtc cgcaaagtgg cgcggggcga
     1441 gtccctcctc gatccgcgga ccacgcagcg ggtgctcgag cgcatccgcc gcgctcccga
     1501 ggaggacgag cgcctcaagg gcctgacccc ccaggagaaa cgcatcctgt cgttgctcgc
     1561 ggagggccgc accaaccgcc agatcgcgga tgagatgttc ctcgccgaga agaccgtcaa
     1621 gaactacgtg tcgaacctgc tgtcgaagat gggcatgagc cgtcgcaccg aggccgccgt
     1681 gtacgcggcg cggttggcgg agcgtcgcgg tacgacctga tcggtcggcc ggttcggggg
     1741 tcgtcgtcga cgtcgggacg ttcggccctg tcgccacggg gtgcactctg gccgaaatgg
     1801 gcgtgtgatc cgtgtcttcc ttctcgacga ccacgagatc gtgcgtcggg gtgtgcggga
     1861 gctgatcgac ggcgaggacg acctcgtggt ggtcggcgag gccggcaccg gtgccgaggc
     1921 gatcgcgcgg ctgccctcga cgcgccccga cgtggcggtg ttggacgtcc agctgggtga
     1981 gggaagcggc gtcgaggtgt gccgcgacat ccggtcgatg atgccggagg tcgggtgcct
     2041 catgctcacg tccttctcgg atgacgaggc ggtcgtcgag agtgtgctcg ccggtgccca
     2101 gggctacgtg ctgaaacagg tcgtcgggtc ggacctcatc gagagcatcc gcgccgtggc
     2161 ggcgggtcgc agcctgatcc ctgccgacgt ggtcgagaag gttcgcgacc acgtgcgcgc
     2221 catccaggct gtggaggacc gcatcgccga gctgggaccc cgcgagcggg ccatcctggc
     2281 gctcctggcc gacggccaca ccaaccgtga gatcggcgag cgcctgttcc tgtcggagaa
     2341 gaccgtcaag aactacatgt cgaacgtgct gcggacgttg ggtatgcacc ggcggaccga
     2401 ggcggcggtg ttcgccgcca aggcggccca acggcgggag aaggaccttc gcctctgacg
     2461 ggcgggcgtc cttggaccga ggatgaagtg actcaccccc gaacggaccg aacagatatg
     2521 gaccccaaca ccgaagatcg agatctggtc gaactcgacg aggacgaatg cgccaccctg
     2581 ctggcgctcg agtcggtcgg tcggctcgcg gtcggcgacg ccaccgctgc tcccgacgtg
     2641 gtgccggtca acttcgtgct ccgcgacggt gacccgatct tccgttccca ccccggtgtc
     2701 ctcctcgacc gggttctcag ctcgcccgtc agcctgcagg tcgaccggtt cgactggttc
     2761 caccgcacgg gctggagtgt gctcgtacac ggcgtcgccg agctgatcga tcccgacatc
     2821 gccggtgccc tcatcgagga gggcgacgac accctcgata cctgggcacc cggcgaccag
     2881 ccggtcctgg tgcgggtgag ggcggagtac ctgagcggtc gccggatcga actccagcag
     2941 atcccgctcg acggacgggg ttacctgtga acggcgcggc gcagcacgga acgtggtctt
     3001 cgcttccgtt gccgatcgac ggctccgacg agcacggtct cggcctcatc gacgaggcca
     3061 cctgtgtgcg cctgctctcc cacggccaca tcggtcgcct cggcttgagc gtcggcagcc
     3121 tgccggtggt gctcccggtg aactacctgc tccaggacca cacgatcgtg ttccggtccg
     3181 aggacggcga caagacccgc gccgccgagc tcggctcggt cgcctgcctc gagatcgacc
     3241 acttcgacct gttcgaacac agcggctgga gtgtcctcgc cacgggccgt ctgtccatcg
     3301 cgccgaacga tcgagcagcg gtgtacgacc ggctcccggt cgtgccgtgg gcgctgcagc
     3361 agtcgagtcg cttcgtcgaa ctgtccgtcg aactcgtctc cggaaggagc atccgatgac
     3421 cgaactgcaa cttgaccatg ccgggctcga gatcttgaac ccggccgaat gccgcgagct
     3481 gctcgcctcg accgcggtcg ggcgcattgc gttcatcgac cggggcgacc cggtgatcct
     3541 gcccgtaacg atcgggatgt gggagggcgc cgtggtcttc acgacggcgc cgggctccaa
     3601 gctcgaggcg gcgatcatga accggcccgt cgcgatcgag gtcgacgact gggatgcgga
     3661 gcgacacgtc gggtggagcg tgttggtgaa gggcatggcg atgatcgtcg atgacggtcg
     3721 cgagatcgac agtctcgacc gcctgtcggt gaagtcgtgg agccgacccg agatccccaa
     3781 ggcgtgggtc cggatcgtcg ccaacgagat caccggacgc cgggtcgtac cgatcagcag
     3841 atgacggtcg ggtgaactcc tgtcgcctcg ggtcggattt cggcgattcc tcggtaccgt
     3901 gaccggatcg gtggtgtccc gaacgaggag ctgagatgac agtgacgagc gggccgacgc
     3961 acggctccca ttcgaccatg ccgcaggcgc cggtcgtgcc cggccccgag gcgcgggccc
     4021 ggcagcacgg ccacggcgaa cagaacggac gcgccgacct ccacgagctg gtcgagtcgc
     4081 tcgccgagcg gacgctccgg ccctccgacg attcccaggc ggcctcgcgc atcctggtcg
     4141 cggcgaacac cgccgccgaa cagctgctgt cggacgctcg ggccgaagcg acggcgctgg
     4201 tcgacaacgc caaggcccgc acggccgagc tgctgaccgt ggcgcaggaa ctcgccgatc
     4261 gcgagctcca cgccgagcgg acccgcgtgt cgggggccgt ggcggcctgg aacgcacagc
     4321 agcgcgagat cgccgcccgc ctcgacgcgt tgcggacgtc gctcgccacg taccaggccg
     4381 acgccgaggc gacgctggca gcgatcgccg acgccctcgc cggtctcgcc gcggtcgaga
     4441 tcgacgcggg cgcggcccgg gcgaccacga ccgtcggccg cgatctggat gccgagccgg
     4501 tgcccgagac ggcggcaccc gccgacctgc cggcgcccgc cgaggccgag ggacgggtca
     4561 tcgcgacccc gcttcccgcc gcgtcgacac ccgcaggtcc gatacccgca ggtccggcaa
     4621 caccggccga ggaacatgac gggccgcacg cccacgttcc cgcccaggcc cgtgatcagg
     4681 ccgacacctc cgaggtcggg gcgcagcgca cctcgctgtt cctcagccct gcggctaccg
     4741 cggcgtcgtc ctcgcaaacc gcgaccaccc gccagggcct gttcggtcgc tgatcgcctc
     4801 gacccggatc atcgcccagt tcggtgtgat ctcaacggtg atcagtgcat cgacgagtcg
     4861 ggcttctggc gttgcgggga ggaaccgacc ggtctgcggc ggtcttcgtg actgtcgccg
     4921 gatcatcgtc gatgtcgagg gtcacccaca cacgtcgtga ccccacgatg ccctcggtga
     4981 gatagagccg gctcgaacag ctgcgtcccg ggctggctca ccgcttcatg ctctcgcgcg
     5041 gcggtgtgag cactcgactc acggcgggtt acttgtacgg cgactcgaca tcctcaggcg
     5101 gatcgtgagg tgcttccgcc tcactagctc acatcccgtt cgacctgctc gtaaaccaga
     5161 cgatgagcac cgcggctccg cttcgccctc gacgcaccca acgacaaggc gatctgaccg
     5221 tcgctggtgg tcagtgtggc gtcggccgag gtcctgcgct cgaggtccgc cctgatgatc
     5281 gttcgagagt ggttcggtcg atggtcgtcc cccagctcgc aacgcgactg ctactcgtac
     5341 tccgcagacg actcggtcgc ggcaccagaa aaaacctacc cagagtcagc cgaacggctt
     5401 aggtcccgcc acggttgtgc cgataggg
//
LOCUS       DAOSOA010000224         5427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15181_length_5427_cov_6.008377, whole genome shotgun sequence.
ACCESSION   DAOSOA010000224 DAOSOA010000000
VERSION     DAOSOA010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15181_length_5427_cov_6.008377"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PKY13_12515"
     CDS             complement(<1..422)
                     /locus_tag="PKY13_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT80808.1"
                     /translation="MGFVIRRIAARVVCFDPDGRILLIRSSDPADRSKPAWWELPGGG
                     IDGGETAEHAIARELAEEAGVRDPVIGPCVWTQHAQFTFAGWDFDQHERIHVATCDGD
                     TTGTLHLEAFEALAFEESRWWDPEEFLAETGPTVPPRLR"
     gene            487..1179
                     /locus_tag="PKY13_12520"
     CDS             487..1179
                     /locus_tag="PKY13_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80809.1"
                     /translation="MSWTVEHHRGGADELHHLGLEAPGRTVLVMELDHAAVALGSTQS
                     EAVLDPAALRSHGPGRDPRAVTRRHSGGGLVVMNPGEVVWVDVVIPAADPLWRVDVGE
                     AFVWLGRVWAEVVAGRLAPVDPPPVVHRGAPRHADLGRAVCFAGVGSGEVVVGDRKVV
                     GLSQRRTRDWARFQCLVHGRFDAEDSMSVLAPHLTTGVFGDRLRARLANGVGVVALDG
                     LVDDLVARLATR"
     gene            1557..1979
                     /locus_tag="PKY13_12525"
     CDS             1557..1979
                     /locus_tag="PKY13_12525"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80810.1"
                     /translation="MSYYGSYDHTVDPKGRVVVPALFRDEFRNGGHLALRRDHIALYD
                     PAGWERFMTKLRELRDAGEVSRPQFNQLMALSSPITPDSQGRFGLPQRLRDTAGITGN
                     AVFVGVDDYLAIHSPETAPIPAGDDFDALMDAFDGLPL"
     gene            1976..2983
                     /gene="rsmH"
                     /locus_tag="PKY13_12530"
     CDS             1976..2983
                     /gene="rsmH"
                     /locus_tag="PKY13_12530"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT80811.1"
                     /translation="MSDGDEHDATSPDDVTSADEFRHVPVMLAEVVALLEPIPAGLVV
                     DATLGGAGHAAAILDARPDVRLLGLDRDLDALAVARRRLARFGDRVHLHHGRFDEMTD
                     VVRSLGEEGVTGVLFDLGVSSPQLDRADRGFSYRHDAPLDMRMDRTQARTAHDVVNEY
                     SAAALADVLDRYGDERFSRRIANAVVANRPVETTTQLADIVRDAIPAPARRTGGHPAK
                     RTFQALRIEVNEELETLTSALDQAIDLLLPGGRCVVLSYHSGEDRIVKDRFRRAETGG
                     CVCPPGLDCVCGAEPKVRLLRRGAQKATAEEIEVNPRASSVRRRAVESVTFKPRPQEV
                     A"
     gene            2983..3438
                     /locus_tag="PKY13_12535"
     CDS             2983..3438
                     /locus_tag="PKY13_12535"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOT80812.1"
                     /translation="MARATSSAAAAKRAPRSAPAAKSARPELAVVTHRRRITPTMVVA
                     MLLAAAVAFGALSVQISLIHRQQRLDAVRTEITELQQSNKELRQKESRLQAPSEILRI
                     ARDELGMVESKPPELVTPAVETVGAVDPAAAPGSSSTSESTDGASAADG"
     assembly_gap    3485..3584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3738..>5427
                     /locus_tag="PKY13_12540"
     CDS             3738..>5427
                     /locus_tag="PKY13_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT80813.1"
                     /translation="MARRRGPQVGLRVRLAIPYFIVVALALLLAARVLWVGIFDADTY
                     EALGLSKRERTIPLHELRGTIFDRNGEALVMSVPTKLVAVDPTLVVDPETTATQIAGI
                     LGVERLAVLDALEKPDSRYSVVARQVDADVAEQLKAAGIPGLTLSEDPQRVAVNGDLA
                     RSVTGSMDRYATTARSGIELLYDEELRATEGKELVQQGVRGSTIPGSERVVRAAEAGS
                     NITLTLDRALQFLAEGELARQVEAVQAKGGTVVLGRPQTGEILAMASVSRTADGQIVQ
                     GSLNQAIRLYEPGSIMKLVTVATAYDMGLVTPDSLIEVPYEITLYDKTIHDSHSHPTE
                     MMSVNQILSGSSNVGIIKIAQLIKAAGGLDALVDSFKSFGFGEYTALGLPKEQEGVLK
                     KPSDWNGSDIGSIPIGQSITVSPVQMWSSYNAIANGGRYVPPKLVRDVVDAQGRHIEP
                     KVPEHAPTAHRVVSTAAAKMVTKSLEDVIDTEQGTGSKFKIPGFNIAAKTGTAYKVQG
                     DGTYGTAANRKYSSSFVGFFPASNPQISIMVMLDEPAYGLHFGATAAGPVFDTLA"
BASE COUNT          799 a         1918 c         1790 g          820 t
ORIGIN      
        1 cggagccggg gcgggacggt cgggccggtc tcggcgagga actcctcggg atcccaccac
       61 cgcgactcct cgaacgccaa cgcctcgaag gcctcgaggt gcagcgtgcc ggtggtatca
      121 ccgtcgcagg tggccacgtg gatgcgctcg tgctggtcga agtcccagcc ggcgaacgtg
      181 aactgggcgt gttgggtcca cacacacggc ccgatgaccg gatcccgcac accggcctcc
      241 tcggccagtt cgcgggcgat cgcgtgctcg gcggtctcgc cgccgtcgat gccaccaccg
      301 ggcagctccc accacgccgg cttcgagcgg tcggccggat cgctcgaccg aatgagcagg
      361 attcgcccgt cgggatcgaa gcacaccact cgtgcggcaa tccggcggat gacaaaaccc
      421 atggcgccga accctagggc gtgagccgga gcgcggcacg ccccgtcccc gacgtagcat
      481 cgccagatgt cgtggacggt cgagcaccac cgtggtggcg ccgacgaatt gcatcacctc
      541 ggcctcgagg cgcccggacg caccgtgctc gtgatggaac tcgaccacgc ggccgtcgcc
      601 ctcgggagca cccagtccga agcggtgctc gacccggccg ccctccggtc acacggtccc
      661 ggtcgcgatc cccgagcggt cacacggcgc cattccggcg gtggactcgt cgtgatgaac
      721 cccggcgagg tcgtgtgggt cgacgtggtc atccccgccg cagatccgct gtggcgggtg
      781 gacgtggggg aggccttcgt ctggctgggc cgcgtgtggg cggaggtcgt ggccggacgg
      841 ctcgcgccgg tcgatccgcc gccggtggtc caccgcggtg cgccccgcca cgccgacctg
      901 ggccgggccg tctgtttcgc cggcgtcggg agcggcgagg tggtggtggg tgaccgcaag
      961 gtggtcggcc tgtcccagcg tcgcacccgt gactgggccc gcttccagtg cctcgtgcac
     1021 ggtcgcttcg atgccgagga ctcgatgtcg gtcctggcac cgcatttgac caccggtgtg
     1081 ttcggtgatc gtctccgcgc ccgccttgcc aacggcgtcg gagtggtcgc gctcgacggg
     1141 ctggtcgacg acctcgtggc ccgcctcgcc acccgctgac gccggtcggt tcacccgccg
     1201 acgctctcag ggaccacccc gggacgcgat ctcgcgggtg ggacgggtgg aggggagtgg
     1261 agtggtgcgc cgaccgcgct ccgtgacgaa cgagtgttcg aaattacaaa actggggtga
     1321 tgtatcgcgc catgtcaggt gatttcgaga ccgaaaaatc ttgaaagttc ccgagaaatc
     1381 gggctccaga gtctgacggg ggaaggaggt ggtggtttat ggtgggtccc cgtggagcag
     1441 agtggggcaa agtggaccaa ccgacgttgg cacctgtttg ttccggcgtc gggtgccgtt
     1501 cattccccga tgttcctcgt ttccgttggt gacatgtggg ccgaccggag ccggcgatga
     1561 gctactacgg atcgtacgac cacaccgtcg acccgaaggg tcgtgttgtc gttccggcgc
     1621 tgttccgcga cgagttccgc aacggcggcc acctcgccct gcgacgtgac cacatcgcgc
     1681 tctacgaccc cgccgggtgg gagcgcttca tgacgaagct ccgcgagctc cgcgacgccg
     1741 gcgaggtctc ccggccccag ttcaaccagc tgatggcgct ctcgtcgccc atcacgccgg
     1801 actcccaggg gcgcttcggc ctcccgcagc gcctgcgtga caccgccggg atcacgggca
     1861 acgccgtgtt cgtcggcgtc gacgactacc tcgccatcca ctcgccggag acggcaccga
     1921 tccccgcggg cgacgacttc gatgccctca tggacgcgtt cgacggattg cccctgtgag
     1981 cgatggagac gagcacgatg cgacgagccc tgacgatgtc acgagcgctg acgagttccg
     2041 gcacgtcccg gtcatgctcg ccgaggtcgt cgcgctcctc gagccgatcc ctgcgggact
     2101 cgtggtcgac gccaccctcg gcggcgccgg tcacgcggcg gccatcttgg acgcacgacc
     2161 ggatgtgcgg ctcctcgggc tcgaccgcga cctcgacgcc ctggcggtcg cccgccggcg
     2221 cctcgcccgt ttcggggacc gggtccacct ccatcacggg aggttcgacg agatgaccga
     2281 tgtggttcgt tcgcttggtg aagaaggggt gaccggagtg ctgttcgacc tgggagtgag
     2341 ctcgccgcaa ctcgaccgcg ccgatcgggg gttctcctac cgtcacgacg cgccgctcga
     2401 catgcggatg gaccgcacgc aggcgcggac cgcccacgac gtcgtcaacg agtactcggc
     2461 cgcggcgctc gccgatgtgc tcgatcgcta cggcgatgag cgcttcagcc gccgtatcgc
     2521 caacgcggtc gtcgccaacc gccccgtcga gacgaccacc cagctcgccg acatcgtccg
     2581 agacgccatc ccagcgccgg cccgccgcac cggtggtcat ccggcgaagc gcacgttcca
     2641 ggccctccgc atcgaggtga acgaggaact cgagacgctg accagcgccc tcgaccaggc
     2701 gatcgatctg ctgctccccg gcggtcggtg tgttgtcctc agctaccact caggcgagga
     2761 ccgcatcgtc aaggaccgct tccgccgcgc cgagaccggc ggctgcgtgt gcccgcccgg
     2821 gctcgactgc gtgtgcggtg ccgaaccgaa ggtccggctg ctccgccgtg gtgcccagaa
     2881 ggccaccgcc gaggagatcg aggtcaaccc ccgagccagc tccgtgcgcc gtcgcgccgt
     2941 cgagtcggtc acgttcaagc cccggcccca agaggtcgcc tgatggcacg agcgacctcg
     3001 tcggccgcag ccgcgaagcg cgctccccgg agcgcgccgg cagcgaagtc ggcgcgcccc
     3061 gagctggcgg tcgtcaccca ccggcgacgc atcacgccca cgatggtcgt ggcgatgttg
     3121 cttgccgccg ccgtcgcgtt cggtgcgctg tcggtgcaga tctcgctgat ccaccggcag
     3181 cagcgactcg acgccgtgcg gaccgagatc accgagctgc agcagtccaa caaggagctc
     3241 cggcagaagg agagccgact gcaggcgccg tcggagatcc tgcgcatcgc ccgtgacgaa
     3301 ctcggcatgg tcgagtccaa gccgcccgag ctcgtgaccc cggcggtcga gaccgtcggc
     3361 gcggtcgatc ctgcggcggc ccccgggtcg tcgtccacct ccgagtcgac cgacggcgcg
     3421 tcggccgccg acggctgatc gcaggacact ccatggctcc ggtcgcgacc tcgacatcgt
     3481 cccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacccgg tcgcgctcct
     3601 cgatccccgg cgccggtgcg cgcggggccg gtggggcggg cacgtcccgc tccaccggcc
     3661 ctcgttcctc cggtccgacc cgccccaccc gtcggacccg cccgactcgt cggacggcgc
     3721 ctcgcccggc gcgcaaggtg gcccgtcgcc gcggccccca ggtcggactt cgtgtccggc
     3781 tcgccatccc gtatttcatc gtcgtggcgc tcgccctgct cctcgcggcg cgggtcctct
     3841 gggtcggcat cttcgacgcc gacacctacg aggcgctcgg gctcagcaag cgtgagcgca
     3901 ccatccccct gcacgaactc cggggcacga tcttcgaccg caacggcgag gccctcgtca
     3961 tgtcggtgcc gaccaagctg gtggccgtcg acccgacgct cgtcgtggat cccgagacga
     4021 ccgcgaccca gatcgccggc atcctcgggg tcgaacggct cgcggtgctc gacgccctcg
     4081 agaagcccga ctcccgctac tccgtcgtcg cccgtcaggt cgacgccgac gtcgccgagc
     4141 agctgaaggc ggcgggcatc cccggcctca cgctgtcgga ggacccccag cgggtcgccg
     4201 tcaacggtga cctggcccgg tcggtgaccg gttcgatgga ccggtacgca acgacggccc
     4261 gatcgggcat cgaactgctc tacgacgagg agctccgcgc caccgagggc aaggagctgg
     4321 tgcagcaggg tgtccgcggc tccaccattc ccgggtccga acgtgtcgtc cgggcggccg
     4381 aggcgggttc caacatcacc ctcactctcg accgggcgct gcagttcctg gccgaaggtg
     4441 aactcgcccg gcaggtcgag gcggtgcagg ccaagggcgg caccgtcgtg ttgggccgcc
     4501 cccagaccgg cgagatcctg gccatggcat cggtgtcacg tacggccgac gggcagatcg
     4561 tgcagggctc gctcaaccag gccattcgcc tctacgagcc cggttcgatc atgaagctcg
     4621 tgacggtcgc gacggcgtac gacatgggtc tcgtcacccc ggactccctc atcgaggtcc
     4681 cctacgagat cacgctctac gacaagacga tccacgactc ccactcgcac cccaccgaga
     4741 tgatgtcggt gaaccagatc ctgtcggggt cgtcgaacgt cggcatcatc aagatcgccc
     4801 agctgatcaa ggcggcgggt gggctcgatg cgctcgtcga ctccttcaag tcgttcgggt
     4861 tcggtgagta caccgccctc ggtcttccga aggaacaaga gggtgtgctc aagaagccgt
     4921 cggactggaa cggctccgac atcggctcga tcccgatcgg ccagtcgatc accgtgtcgc
     4981 cggtccagat gtggtcgtcg tacaacgcga tcgccaacgg cgggcggtac gtgccgccga
     5041 agctcgtccg tgacgtcgtc gacgcccagg gtcgccacat cgagccgaag gtcccggagc
     5101 acgccccgac ggctcatcgt gtcgtgtcca cggcggcggc caagatggtg accaagtcgc
     5161 tcgaggacgt gatcgacacc gagcagggca cgggcagcaa gttcaagatc ccgggcttca
     5221 acatcgcggc caagaccggc acggcgtaca aggtccaggg cgacggcacc tacggcacgg
     5281 cggcgaaccg caagtactcc tcgagtttcg tcgggttctt cccggcgtcg aacccccaga
     5341 tctcgatcat ggtgatgctc gacgagccgg cctacggcct gcacttcggc gccaccgccg
     5401 ccgggccggt gttcgacacc ctggcca
//
LOCUS       DAOSOA010000225         5420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15202_length_5420_cov_6.454986, whole genome shotgun sequence.
ACCESSION   DAOSOA010000225 DAOSOA010000000
VERSION     DAOSOA010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15202_length_5420_cov_6.454986"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKY13_12545"
     CDS             <1..315
                     /locus_tag="PKY13_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80814.1"
                     /translation="AVGEAVSVAVRDALFERGQDISDPDVLEHIADSYGLGPVMPSDD
                     AQVDTDWEDGRRRDVVGSPHFFLGDDGVFCPTLDITKVDGRLHIAIDQPAFDAFAEQV
                     FT"
     gene            complement(464..1057)
                     /locus_tag="PKY13_12550"
     CDS             complement(464..1057)
                     /locus_tag="PKY13_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT80815.1"
                     /translation="MPKIAADSVHEHVARQEAAVIEAAIRLFNERGVRNVNLGDIAKE
                     VGLARNSLYRYFPDKGHILAAWFRITIAPLVELCAVIAEADEPPAHRIARWVDAQFDY
                     LTAPDHAAMLMASNEMTNLSDDVRAELGAGHRELYASLARVLDEAFGGGPERNTDIVT
                     MLIASVVRNAAEQARAGADIDAVRAELSRVTSALVAT"
     assembly_gap    1079..1178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1179..1597)
                     /locus_tag="PKY13_12555"
     CDS             complement(<1179..1597)
                     /locus_tag="PKY13_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT80816.1"
                     /translation="MNQNAALELQGVDVVVPDGTETLRILDGVDLRVEAGEVVKLTGA
                     SGSGKSTLLAVAALLRAPTSGRVILDGTDTAHLPARRLAELRNQRLGMVFQSSNLFPS
                     LTAVEQVELVAHIAGTLNGPARRRARELLASVGLASRA"
     gene            complement(1606..2706)
                     /locus_tag="PKY13_12560"
     CDS             complement(1606..2706)
                     /locus_tag="PKY13_12560"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT80817.1"
                     /translation="MFLALRDLRVARGRFVLVGVVIGLVALLTTVLSGLANGLVDDGI
                     SGLRALPLTHLALQDGAESSFSRSTLTDKNLDPWKELAAKSGGDVEVSPLGVSFFNAK
                     RANGDTIDIALFGVEPGTFLTPRSDAQAALNGTPGLVLSHEFESEGVKVGDELTIVGV
                     DTTLPVLGFTYAGSYGHVDIAFSSLATWQSLVYGDNARGRFSAIAVRTEGDASFAAVD
                     KAAGTEVETKEASYAGSPGFSAESATMTLIRGFLLVISALIVGAFFTVWTIQRTRQIG
                     LLKALGASNGYVLRDALGQLAIVLVSAVLVGAAIALGVGALVPEEVPFSLEATPIVTS
                     VLALVLLGMAGSLVAVRRITSVDPIVALSSEN"
     assembly_gap    2772..2871
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2959..5139)
                     /locus_tag="PKY13_12565"
     CDS             complement(2959..5139)
                     /locus_tag="PKY13_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80818.1"
                     /translation="MRATRRQFLFASAAGAGAVWLPGCAPGEVPPVPSPTCAAPTIGG
                     PAPLPAPGSVGLIDEVAFQQRADAYLTVATQSLQPTSGASVATHLVRAQREPGFVWDP
                     SAVTAALVNPDPYYDTADFDLMNYQWILRLGAGTLPAATITAIEDAIAGFRYRYDDPL
                     PAGDVDNKWFWSENHRMIFAVDEYLGGLALPDRVFTYTGLTGAQHAARTRQPLLDWLD
                     ERARFGFFEWHSNTYMKYDYAPLLTLVEFADDPEIVSRAAAVIDLCFYDVATHTIRGA
                     YGVTHGRAYKVSKTNSLVESSFQTAKLLFDTTDQPFNGPDIGPAFLIGSTKYRLPAAI
                     QEAARSDEAATIRERHGVVLDPHEEFSLDPQGAYGYEYNAANLPFWWSQGALTAWQMV
                     PATLDAAKRWQLFDSELFQKFSAVKQFADLNPGILQVVIRELAPFAAAGVLGEAHTYT
                     WRSPDVMLSSVVDHRFGDAMEQVHAWQATLDPEALVFTTHPSNDLRPSADWGNDNGYW
                     TGTASMPRTAQVGRAAVHIYRPRYASPTDDLLGPLFGYEHFTHAFFPQDRFDEIVEQD
                     GWVIGRKGEGYVALWSERPAHWRAYAPGESTGGHTLPFDLVAPGGADNVWICEVGRAA
                     DHPALSGSAAFGAFVAAVTAAAPVVVRTGPEIDVTYTSPIEGTLRLGTQGGFSLDGTA
                     RPLRDHPRHSSPWAEQCALGKGMDISAGGSRLELNVGTGGRRVS"
     gene            5236..>5420
                     /locus_tag="PKY13_12570"
     CDS             5236..>5420
                     /locus_tag="PKY13_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80819.1"
                     /translation="MATTGVVVGAGDRGFDAYATLLLDEPELGRIVAVADPDAGRRAR
                     FAERYSLGTADCFDGWES"
BASE COUNT          805 a         1703 c         1932 g          780 t
ORIGIN      
        1 gcggtcggcg aggccgtcag cgtggccgtg cgcgacgcgc tgttcgaacg aggtcaggac
       61 atcagcgacc ccgacgtcct cgagcacatc gccgactcgt acgggctcgg gccggtcatg
      121 ccgtccgacg acgcgcaggt cgacaccgat tgggaggacg gccgccgccg cgacgtggtc
      181 gggtcgccgc acttcttcct gggcgacgac ggcgtcttct gtccgacgct cgacatcacc
      241 aaggtcgacg gacggctgca catcgccatc gaccaaccgg cgttcgacgc gttcgccgag
      301 caggtcttca cctgaaccac ggtcggggac cttcgcccca gtcgcggcgt cgttccgcgg
      361 tccgacactc ggatcgacgg gccgaatctc ctgctcacgg gacgtgcgct gcccgccgat
      421 tggtggggtg gcggcgcgac ccgaccgtcg gggttgctgg ccctcaggtc gcgacgaggg
      481 cggacgtgac ccgcgacagc tcggcacgaa cggcgtcgat gtcggcaccg gcccgggcct
      541 gctcggccgc gttccgcacg accgacgcga tcagcatcgt gacgatgtcg gtgttgcgct
      601 ccggaccgcc gccgaaggcc tcgtcgagca ctcgggcgag cgacgcgtag agctcccggt
      661 gaccggcgcc caactcggcg cggacgtcgt cggacaaatt ggtcatctcg ttggacgcca
      721 tgagcatcgc cgcatggtcg ggcgcggtga gatagtcgaa ctgggcgtcg acccagcggg
      781 caatgcgatg cgcgggaggt tcgtcggcct cggcgatcac cgcgcacagc tcgacgagtg
      841 gggcgatcgt gatgcggaac caggctgcga ggatgtggcc cttgtcgggg aagtaccgat
      901 agagcgagtt gcgggcgagg ccgacctcct tggcgatgtc gccgaggttg acgttgcgca
      961 cgccacgttc gttgaacaga cggatcgccg cctcgatgac cgccgcctcc tgacgcgcga
     1021 cgtgctcgtg cacggagtcg gcggcgatct tgggcatcgg tcagacgata gcggtgtgnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tcgtgacgcg agaccgaccg
     1201 aggccagcag ctcgcgggcg cgtcgtcggg ccggcccgtt gagcgtgccg gcgatgtggg
     1261 cgacgagttc gacctgttcg accgcggtga gggacgggaa caggttcgac gactggaaca
     1321 ccatgccgag ccgctggttg cggagctcgg cgagccgacg ggccggcagg tgggcggtgt
     1381 cggtgccgtc gaggatgacc cggcccgacg tgggagcccg gagcaaggcg gcgacggcca
     1441 gcagggtcga cttccccgag cccgatgcgc cggtcagctt cacgacctca ccggcttcga
     1501 cgcgcaggtc gacaccgtcg aggatcctca gcgtctcggt gccgtcgggc acgacgacgt
     1561 cgaccccctg gagctcgagt gcggcgttct ggttcatcag gggcctcagt tctcgctgct
     1621 cagggcaacg atcgggtcga cggacgtgat gcggcggacg gcgacgaggc tgccggccat
     1681 gccgaggagg acgagggcca gtaccgacgt gacgatcggt gttgcctcca ggctgaaggg
     1741 cacctcctcg ggcacgaggg cgccgacgcc cagcgcgatc gccgcgccga cgaggacagc
     1801 gctgaccaac acgatcgcga gttgtccgag cgcgtcgcgg aggacgtagc cgttggacgc
     1861 accgagggcc ttgaggagac cgatctggcg ggtgcgctgg atcgtccaga cggtgaagaa
     1921 ggcaccgacg atgagcgccg agatgacgag caggaacccg cggatgaggg tcatcgttgc
     1981 gctctcggcg gagaagccgg gcgagccggc gtaggaggcc tccttggtct cgacctcggt
     2041 gccggcggcc ttgtcgaccg cggcgaacga cgcgtcgccc tcggtgcgca cggcgatcgc
     2101 tgagaaacgg ccccgagcgt tgtcgccgta caccagcgac tgccacgtcg cgagcgacga
     2161 gaaggcgatg tcgacgtggc cgtaggaccc ggcgtaggtg aacccgagca ccgggagggt
     2221 ggtgtcgacg ccgacgatcg tgagctcgtc gccgaccttc acgccctcgg actcgaactc
     2281 gtggctcagg accagcccgg gcgtgccgtt gagcgcggcc tgggcgtcgg agcgaggggt
     2341 gaggaaggtg ccgggttcca cgccgaagag ggcgatgtcg atggtgtcac cgttggcgcg
     2401 cttcgcgttg aagaacgaca caccgagggg tgagacctcg acgtcgccgc ctgacttcgc
     2461 ggcgagttcc ttccacggat cgaggttctt gtcggtgagg gtggagcgac tgaagctgct
     2521 ctcggcacca tcctgcaggg cgaggtgggt gaggggcagc gctcggagcc ccgagatgcc
     2581 gtcgtcgacg agcccattgg ccaggccgga caggacggtc gtgaggaggg cgaccagccc
     2641 gatgaccacg ccgacgagga cgaaccgacc tcgggcgacg cggagatctc tcaacgcaag
     2701 gaacatgatc cacctcgttc gaatcttggc gacaacatgt cgacatcata tcgacgcagt
     2761 gtcggcatgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcaccttcg
     2881 tcacgttcga cctccgccag gcgcaggcgg cccgatcgct cgggttcacc gtgcggggag
     2941 cttgaccgac tcgggcgatc agctcacgcg gcgcccgccg gtcccgacgt tcagctcgag
     3001 gcgcgagcca ccggcgctga tgtccatccc cttgcccagg gcgcactgct cggcccacgg
     3061 gctcgagtgg cgggggtggt cgcggagcgg ccgggcggtt ccgtcgagcg agaagccgcc
     3121 ctgcgttccg aggcggagcg tgccctcgat cggcgaggtg tacgtcacgt cgatctcggg
     3181 accggtgcga acgacgaccg gggcggccgc ggtcacagcg gcgacgaacg caccgaacgc
     3241 ggcgctgccg gacagcgcag ggtgatccgc ggcgcggccg acctcgcaga tccacacgtt
     3301 gtcggcgccg cccggagcga cgagatcgaa cggcagggtg tgaccgcccg tcgactcgcc
     3361 cggcgcgtac gcccgccagt gcgccggccg ctcggaccac agcgcgacgt acccctcgcc
     3421 cttgcggccg atgacccatc cgtcctgctc gacgatctcg tcgaagcggt cctgcgggaa
     3481 gaacgcatgg gtgaagtgct cgtagccgaa caacggcccg aggaggtcgt cggtgggcga
     3541 cgcgtaccgc gggcggtaga tgtgcacggc agcccgtccg acctgggccg tgcgcggcat
     3601 cgacgccgtg ccggtccagt agccgttgtc gttgccccag tcggccgacg gacggaggtc
     3661 gttggacgga tgggtcgtga acacgagcgc ctcgggatcg agcgtcgcct gccacgcgtg
     3721 cacctgctcc atcgcgtcac cgaaccggtg gtcgacgacc gacgagagca tcacgtcggg
     3781 gctccgccag gtgtaggtgt gcgcctcgcc gaggacgccc gcggccgcga acggcgcgag
     3841 ttcacggatg accacctgga gaatgccggg gttcaggtcg gcgaactgct tcaccgcgct
     3901 gaacttctgg aacagctcgg agtcgaagag ctgccagcgc ttggcggcat cgagggtcgc
     3961 gggcaccatc tgccacgcgg tgagcgcccc ttgcgaccac cagaacggga ggttcgcggc
     4021 gttgtactcg tagccgtacg cgccctgggg gtcgagcgag aactcctcgt gggggtcgag
     4081 caccacgccg tgccgctcgc ggatcgtcgc ggcctcgtcg ctccgggccg cctcctggat
     4141 cgcggcgggc aggcggtact tcgtggaacc gatgaggaag gccgggccga tgtcgggccc
     4201 gttgaacggc tgatcggtcg tgtcgaacag cagcttggcc gtctggaacg acgactcgac
     4261 gagcgagttg gtcttcgaga ccttgtaggc gcggccgtgg gtcacgccgt aggcgccacg
     4321 gatcgtgtgc gtggccacgt cgtagaaaca caggtcgatc acggcagccg cccggctgac
     4381 gatctcgggg tcgtcggcga actcgacgag ggtcaggagc ggcgcgtagt cgtatttcat
     4441 gtaggtgttc gagtgccact cgaagaaccc gaagcgggcg cgttcgtcga gccagtcgag
     4501 cagcggttgg cgggtccgag cggcgtgctg cgctccggtg agcccggtgt aggtgaacac
     4561 ccggtcgggt agagcgaggc caccgaggta ctcgtcgacc gcgaagatca tccggtggtt
     4621 ctccgaccag aaccacttgt tgtcgacgtc tcccgcgggg agcgggtcgt cgtagcggta
     4681 ccggaacccg gcgatggcgt cctcgatcgc ggtgatcgtc gccgccggga gcgttcctgc
     4741 gccgaggcgc aggatccact ggtagttcat gagatcgaag tcggcggtgt cgtagtacgg
     4801 atcggggttg acgagcgccg cggtcacggc gctcgggtcc cagacgaaac cgggttcacg
     4861 ctgcgctcgc acgagatggg tggcgacgct ggcaccgctc gtcggttgca gcgactgggt
     4921 cgccacggtc aggtaggcgt cggctcgctg ctggaacgcc acctcgtcga tgaggccgac
     4981 gctgccgggg gccggcaacg gggcgggccc gccgatggtc ggcgccgcgc acgtcggcga
     5041 cggcacgggc ggcacctcgc cgggcgcgca ccccggcaac cacacggcgc ccgcgccggc
     5101 agcgctcgcg aacaggaact gacggcgtgt cgcccgcatt gcttctcccc ccggttgcca
     5161 catgccatgt gactcgtgtc agcttgcccc atcgtgggtc ggtcggccac cccggaagtc
     5221 ggccatgatg tcggcgtggc gacgacaggt gtggtggtgg gtgcgggcga ccgcgggttc
     5281 gacgcgtacg cgacactcct gctcgacgaa cccgagctgg gccgcatcgt cgcggtcgcc
     5341 gaccccgatg ccggtcgacg cgcacggttc gccgagcgct actccctggg gacggccgac
     5401 tgcttcgacg gctgggagtc
//
LOCUS       DAOSOA010000226         5417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15210_length_5417_cov_6.623834, whole genome shotgun sequence.
ACCESSION   DAOSOA010000226 DAOSOA010000000
VERSION     DAOSOA010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15210_length_5417_cov_6.623834"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PKY13_12575"
     CDS             complement(<1..640)
                     /locus_tag="PKY13_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT80820.1"
                     /translation="MVAGSASDDVGRDELLVRVARAVSRSNGHFAVLVDGALTVKWAS
                     ETVSSVLGWPDVVGRNVIGLIHPDDVELALLGIMHHTTNAEAYSQLDRATMVDPVTVR
                     LQHLDGSWVQVQVTLVNHLADPEIGGLFGICQQVVDRSDMELAIDLMSRNAAIERVLP
                     VVARYAERTGAGQRCQVIWWPEGETRIEVAPDSAPLPGPPTTMVETTRSTRAA"
     gene            769..1245
                     /locus_tag="PKY13_12580"
     CDS             769..1245
                     /locus_tag="PKY13_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80821.1"
                     /translation="MLRRTLIVTVSLAVGLLSACAPEWGGGSSPTTTLVPPSTGQTTH
                     FTYAGQKGSCDQEIVLVGARIRPCQGLGNTTTSVIVNSKVLDPWCTDFFAYYPVTKVT
                     GAAQQGNATRQLVQTDTTPGTPAFDTGFDVANGPIIVTITELSVDTAGGLECGWFG"
     gene            complement(1242..2027)
                     /locus_tag="PKY13_12585"
     CDS             complement(1242..2027)
                     /locus_tag="PKY13_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOT80822.1"
                     /translation="MTTTAEERTQGHLARIAEHGYTVIEGVLDAAQCDALLTDLDRLE
                     RDLEIRPAGNSFEGANTTRVYNLLAHGSMWESVPIEPVVLPVVEGVLDAGCLVSSLSS
                     VSIAPGEIAQPIHADDQMIPLPKPHPPTVCNSMWALTEFTEANGATRIIPGTHLADRS
                     PTYGQHYDSIPAEMPRGSVLVWHGSLWHGGGANRTEERRVGIAMNYCAGWIRQQENQQ
                     LGLRPELVETFPPRLQELVGYGTYQGLIGHIDRRTPAKRLFGN"
     gene            complement(2071..3003)
                     /locus_tag="PKY13_12590"
     CDS             complement(2071..3003)
                     /locus_tag="PKY13_12590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT80823.1"
                     /translation="MPQALRPRSLFTVAALAITTLTACAPPSPAPSPTTAPTTVGPPT
                     RNIVFPLARSVKYSDTFGAGRSGGRSHEGQDLMAPKGTVAVAAAAGTVTMVRHSSDGL
                     SGNMLRITDPEGWQYVYIHLNNDTPGTDDGANVFEQAFVDGVRQGQQVVPGEPIGYVG
                     DSGNAESTGSHLHFEIRSPDGAAVNAYSSLVAAPVAPLSTAQVEGNAPIGSVDAVVPG
                     AGELHVSGWALDRVVDVSVPVSVYVDGNPKVTSIAKLVRSDLAAAFPGRGEQHGFDIG
                     VAGVSAGSHRVCVIAHNAGAGGSARLGCSTVTVG"
     gene            complement(3118..4320)
                     /locus_tag="PKY13_12595"
     CDS             complement(3118..4320)
                     /locus_tag="PKY13_12595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOT80824.1"
                     /translation="MTPDALRHIRGGHPWVFESSIRSVSHDGAPGDLAVVFGDRREFV
                     AIGLWDPGSPIRLRILHSGSPSAVDGSFWRARLASALGRRAALIADPATTGFRLVHGE
                     NDAMSGFVADLYGSTVVVKLYSAAWFAHLPAVLEALVDATALAGLDVDRIVVRLSRNL
                     QAGGRTGLRDGLVVLGEEPLRPVEFLENGLRFEADVIGGQKTGHFLDQRENRARVRDL
                     AAGCRVLDVFACTGGFSLHAAAGGAINVTSVDLSRRSLATAEANFAHNATNPAVAAAR
                     HTTTVGDAFDVMDDLRGRRRRFDLVVVDPPSFAQKEPDRDGAIEAYGRLADLGLDLVE
                     DGGTYVQSSCSSRVSTDEFRAAVRGAAVRSGRRVTVIGETAHAADHPIGFAQGAYLKS
                     LFLTVGAR"
     gene            4494..4928
                     /locus_tag="PKY13_12600"
     CDS             4494..4928
                     /locus_tag="PKY13_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT80825.1"
                     /translation="MRLLGALAALLVVIGATAGCESTEQERNEVIALVNKSRAENGLG
                     QLKDDVTLDMKADAWAQRLRDACSLSHSKLSDGAPKQWLKLGENVGYGGTIAQIHDAY
                     LNSPGHKANIMDPAFTSMGAAAVWGDCEGQHRVFTVQVFMKS"
     gene            complement(4938..>5417)
                     /locus_tag="PKY13_12605"
     CDS             complement(4938..>5417)
                     /locus_tag="PKY13_12605"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT80826.1"
                     /translation="VPRRPVPPLRGRYDLCGLDLARVSSVDALVVDVVGTTAIGAEVF
                     DGLDVAGRAVLVRTDHSRRWGTPAYVERHPHLLAAAADVLVVGGAACVGIDSLNIDST
                     DDSRRPVHSILLAAGIPIVEHLTGLGQLPPSGFTFTAVPPKLEGMGTFTVRAHAIVP"
BASE COUNT          900 a         1897 c         1813 g          807 t
ORIGIN      
        1 gcgccgccct cgtcgaccgc gtcgtctcca ccatcgtcgt cggcggcccc gggagcggcg
       61 ctgagtcggg cgcgacctcg atccgggtct caccctcggg ccaccagatc acctgacaac
      121 gctgcccggc gccggtccgt tcggcgtacc gagcgacgac cggcagcacc cgctcgatcg
      181 cggcgttccg actcatcagg tcgatcgcca gttccatgtc ggaccgatcg accacctgct
      241 gacagattcc gaacaaccca ccgatctcgg ggtcggcgag atggttcacg agggtgacct
      301 gcacctgcac ccaggatccg tcgagatgct gcaggcgcac cgtgaccggg tcgaccatcg
      361 tcgcccggtc gagctgcgag tacgcctcgg cgttggtggt gtggtgcatg atcccgagca
      421 gcgcgagctc gacgtcatcg gggtggatca ggccgatcac gttgcggccg acgacgtcgg
      481 gccaccccaa caccgacgac accgtttccg atgcccactt caccgtcaac gctccgtcca
      541 ccaggaccgc gaaatgcccg ttgctccggg agaccgcacg ggcaacccgg accaacagct
      601 cgtcgcggcc gacgtcgtcc gaagccgatc ccgccaccat ccccccagca tcggcatggg
      661 cacgctccct cctgagaaag tcgcggcccg gaggagttcg cggacctcgc cgataccctg
      721 atctcgccgg tccgaggggc cgtcgatgcg tacctgggag ggtcgagaat gttgcgtcga
      781 acgctcatcg tgacggtcag cctcgcggtc ggactgctgt ccgcctgcgc gccggaatgg
      841 ggtggcggga gcagcccgac cacaacgctc gtgccaccgt cgaccggcca gaccacccac
      901 ttcacctacg ccgggcagaa gggttcctgc gaccaggaga tcgtcctcgt cggcgctcgg
      961 atccgaccct gccaggggct ggggaacacg acgacgagtg tcatcgtgaa ctcgaaggtg
     1021 ctcgatccgt ggtgcaccga cttcttcgcc tactaccccg tcacgaaggt gaccggcgcc
     1081 gcccagcagg gcaacgccac ccggcagctg gtccagaccg acaccacgcc aggcacgccc
     1141 gcgttcgaca ccggcttcga cgtcgccaac ggcccgatca tcgtcacgat caccgagctc
     1201 agcgtggaca ccgccggtgg cctcgagtgc ggctggttcg gttagttccc gaacaaccgc
     1261 ttcgccggtg tccgccggtc gatgtgaccg atgaggccct ggtaggtgcc gtatccgacg
     1321 agttcctgca gccgcggtgg gaacgtctcc acgagctccg gtcgcagacc gagctgctgg
     1381 ttctcctgtt ggcggatcca accggcgcag tagttcatcg caatcccgac gcgccgttct
     1441 tcggtgcggt tcgctccccc tccgtgccac aggctgccgt gccacacgag caccgagccc
     1501 cgtggcatct cggcggggat cgagtcgtag tgctgtccgt acgtcggcga acggtcagcg
     1561 aggtgggtgc ctgggatgat gcgcgttgcc ccgttcgcct ccgtgaactc cgtcagcgcc
     1621 cacatcgagt tgcagaccgt cggcgggtgc ggcttgggca gcgggatcat ctgatcgtca
     1681 gcgtggatcg gctgggcgat ctcaccgggt gcgatcgaga ccgacgacag cgacgacacc
     1741 agacacccgg cgtcgagcac cccttcgacg acggggagga cgaccggttc gatcggcacc
     1801 gactcccaca tcgagccgtg agcgagcagg ttgtacacgc gggtggtgtt ggccccctcg
     1861 aaactgttgc cggcaggccg gatctcgagg tcgcgttcga ggcggtcgag gtcggtcagc
     1921 agggcgtcgc actgcgccgc gtccagcacg ccctcgatca cggtgtaccc gtgttcggcg
     1981 atccgggcca ggtggccctg ggtccgttcc tcggccgttg tcgtcatcag agtcgagcct
     2041 acgacgggct ctgccgccgc gacgggccga tcagccgacc gtcacggtcg agcatccgag
     2101 gcgggccgag ccgccggcgc cggcgttgtg ggcgatcaca cagacccgat gggaaccggc
     2161 cgacaccccg gcgaccccga tgtcgaagcc gtgttgctcc ccgcgaccgg ggaacgcggc
     2221 ggccaggtcg gaccggacga gcttcgcgat cgacgtcacc ttggggttgc cgtccacgta
     2281 cacgctcacc ggcaccgaca cgtcgaccac ccggtcgagg gcccagcccg agacgtgcag
     2341 ttctccggct ccgggcacca cggcatcgac cgagccgatc ggtgcgttgc cctcgacctg
     2401 cgcggtgctg agcggggcaa cgggcgccgc cacgagactg ctgtacgcgt tgaccgcggc
     2461 cccgtcagga ctgcggatct cgaagtgcag gtgcgagccc gtcgactcgg cgttgcccga
     2521 atcgcccacg tagccgatcg gctcgccggg cacgacctgc tggccctggc ggacgccgtc
     2581 gacgaacgct tgctcgaaca cgttggcgcc atcgtcggtg cccggcgtgt cgttgttgag
     2641 gtggatgtag acgtactgcc atccctcggg gtcggtgatg cgcagcatgt tgcccgacag
     2701 gccatcgctc gagtgacgca ccatcgtcac tgttcccgcc gctgcggcca cggcaacggt
     2761 gcccttcggc gccatcaggt cctggccctc gtgggaccgc cccccggatc gtcctgcgcc
     2821 gaaggtgtcg ctgtacttca ccgagcgtgc gagcgggaac acgatgttgc gcgtgggcgg
     2881 cccgacagtc gtcggcgccg tcgtcggcga gggagcgggt gagggcggcg cacaggccgt
     2941 aagggtcgtg atcgccaatg cagcgacggt gaacagggac cgaggccgga gggcctgagg
     3001 catgggaact ccgtgggacg tggatcgagc accctgcatc ggtcgtcgcc ggctccattc
     3061 ttgagcgcgg aaggatgttc agccttcgat tcccccgttc ggtggagaag ccgctggtca
     3121 gcgagccccg accgtgagga acaacgactt gagatacgca ccctgggcaa acccgatcgg
     3181 atgatcggcc gcgtgcgccg tctccccgat gaccgtgacg cggcggccgg accgcaccgc
     3241 cgcaccgcgc accgctgcac ggaactcatc ggtgctcacc cgactcgaac acgacgactg
     3301 cacgtacgtt ccgccgtcct cgacgaggtc gaggccgagg tcggccagtc gtccgtacgc
     3361 ctcgatcgca ccgtctcggt ccggctcctt ctgcgcgaac gacggggggt ccacgacgac
     3421 gaggtcgaac cggcgacgtc gcccacggag gtcgtccatc acgtcgaagg cgtccccgac
     3481 cgttgtggtg tgccgggcgg cggcgacggc gggattcgtg gcgttgtggg cgaagttcgc
     3541 ctcggcggtc gcaagcgaac ggcggctcaa gtcgacgctg gtgacgttga tcgcaccgcc
     3601 cgcagccgcg tgcagcgaga acccaccggt gcacgcgaac acatcgagca cccggcaccc
     3661 cgcggcgagg tcgcgcaccc gggcacggtt ctcgcgctgg tcgaggaagt gaccggtctt
     3721 ctggccgccg atgacgtccg cctcgaaccg gagcccgttc tcgaggaact cgaccggacg
     3781 caacggctcc tccccgagca cgaccagacc gtcacgaagc ccggttcggc caccggcctg
     3841 caggttgcga ctgagccgaa cgacgatgcg atccacgtcc aggccggcga gcgccgtcgc
     3901 gtcgaccagc gcctcgagca ccgcggggag gtgggcgaac caggcggccg agtacagctt
     3961 cacgaccacg gtggatccgt agaggtcggc gacgaacccc gacatggcgt cgttctcgcc
     4021 gtggacgagc cggaagccgg tggtcgcggg gtccgcaatg agggccgccc ggcggcccag
     4081 cgccgaggcc agtcgcgccc gccagaacga tccatcgacg gcgctggggc tcccggaatg
     4141 gagaatccgc agtcggatcg gggagcccgg gtcccacagc ccgatggcga cgaactccct
     4201 ccgatcgccg aacaccaccg caaggtcacc cggggccccg tcgtggctca ccgaccggat
     4261 cgacgactcg aagacccacg ggtgaccacc acgaatatgg cgcagggcgt caggggtcac
     4321 cctgaccgcc aatcggcggg atgacggtgt cggaaggtcg gaaggaaggt tcacgggaac
     4381 agcgaagcta ccgcgcctct ctgaaaaact gctcaaggtc gtttcacagg cccgccgata
     4441 gcttcaacgt gcctcgatcc tcaactctcc gaccgtctgg ccgacgtagc ccgttgcgcc
     4501 tgctcggtgc cctggccgcc cttctcgtcg tcatcggcgc cacggcgggc tgcgagtcga
     4561 ccgaacagga acgaaacgaa gtcatcgccc tcgtgaacaa atcgcgggcg gagaacggtc
     4621 tcggccaact caaggacgac gtcacgctcg acatgaaggc cgacgcctgg gcgcagaggc
     4681 tccgcgacgc gtgttccctc tcacactcga agctgagcga cggcgctccg aagcagtggc
     4741 tgaagctcgg cgagaacgtc ggctacggcg gcaccatcgc gcagattcac gacgcctacc
     4801 tcaactcccc cggccacaag gccaacatca tggacccggc cttcacgtcg atgggggccg
     4861 cagcggtctg gggtgattgc gagggtcagc accgggtgtt caccgtccag gtcttcatga
     4921 agtcctgagg tcgtggctca cgggacgatg gcgtgggcac ggaccgtgaa cgtgcccatc
     4981 ccctccaact tcggcggcac agccgtgaac gtgaaccccg agggcggcag ctgaccgagg
     5041 ccggtcagat gctcgacgat cgggatgccc gcagcgagca ggatcgaatg caccggccga
     5101 cgggaatcgt cggtggagtc gatgttgagc gagtcgatcc cgacgcaggc cgctccgccc
     5161 accaccagga cgtccgccgc ggcggcgagc aggtgcgggt gccgctcgac ataggccggt
     5221 gtgccccacc tacgactgtg gtcggttctc acgagcacgg cacgtccggc gacgtcgaga
     5281 ccgtcgaaca cctccgctcc gatcgcggtc gtcccgacga cgtcgacgac caacgcatcg
     5341 acgctggaca cccgagcgag gtcgagcccg cagaggtcgt atcgtccgcg taacggtgga
     5401 acgggacgtc gaggtac
//
LOCUS       DAOSOA010000227         5417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15212_length_5417_cov_4.800634, whole genome shotgun sequence.
ACCESSION   DAOSOA010000227 DAOSOA010000000
VERSION     DAOSOA010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15212_length_5417_cov_4.800634"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1157
                     /locus_tag="PKY13_12610"
     CDS             168..1157
                     /locus_tag="PKY13_12610"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT80827.1"
                     /translation="MSANLRADDVGASPSQPSFQQLFGVICDNIRRVIRGKDSVVELA
                     VLCMIAEGHLLIEDVPGVGKTSLAKALAATVGGSFGRIQFTPDLLPTDVLGTSVWNQQ
                     MGHFEFRAGPVFNNVVLADEVNRSSPKTQSALLEAMAESQVTSDGVTRGLTRPFMVIA
                     TQNPLEYHGTYPLPESQLDRFLLKLSIGYPARADETLILNSEGADPGLAALAPVVSVG
                     HITAMADHCRQIHVSPTLTGYLIDLAMATRTHRALALGMSPRATLGLKRAVQVRAAAQ
                     GRDHGTADDVKALAVPALAHRLVVAPEAQIQGVDPTAVLNEILGTVAVPRDAG"
     gene            1147..2367
                     /locus_tag="PKY13_12615"
     CDS             1147..2367
                     /locus_tag="PKY13_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT80828.1"
                     /translation="MPVESRARFGLTRSGKAAAVGGAVTLVAGRVAGIEELSVAGVCL
                     LVLVAASLLVVRAAGSDLAVRRTVRPRRVEVGDQFTVSLQIHNGRSGLRLPLVVGDSL
                     DGRRVVQLSLPQRRKDPACVAEYTVGASRRGLATFGPVTIRSLDPFAFARTDLVVPAL
                     IDVIVLPQIHRLGLSGAVAADDPSGGLRHRRQIATVSEEFDTLREYVPGDDVRRVHWA
                     STARLGRPMVRTHQQPSQRRTTVVLDDRADAYPPSRGTAPFERAISAAASALVACREQ
                     GDLVRLVCAGGSDSDYFDDPQRMDTVLDQLAAASTSAVGSLRGALATATAVGSGDRVV
                     VCTGTIGADDRAAISGSASRGGEHVAITTGDGAPATSEAKVLNVSFGEGDDLDRRWQA
                     AVRARAAGPVRVGA"
     gene            2364..4685
                     /locus_tag="PKY13_12620"
     CDS             2364..4685
                     /locus_tag="PKY13_12620"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HOT80829.1"
                     /translation="MTALAPPPPPPPPAPAPAEPTTASQVRRGTPNLGLGAGVDVALF
                     AVTAAVAVALVRAFRSWDQLWPLLAVVAIAHGLSVLGRALRLGATLATALLLISGIVV
                     ITLVYYRAQSWYGLPSAEVFRQAFDDAHVAFGPFQKLVAPVDDLPGFTVTFAAGLWLM
                     AVFADLAAGRSDAPLQAVAPHVATFVFSSVLLLGRHALLSTAVFVTALALYRLAVRAA
                     RLRVAAGTRPGQRVVWVVGVPLLAAVLLASGLFAQASPTDSGGMVDLRSIGRGPKARV
                     VESPLVSLDSLLRGESNDVLFTVTAPTPHYWRLTALDDFDGRSWSASAKYRNLDSGER
                     IPSPWDEDVAVERERIDVEIGDLESDWLPSLYAPTGVDAPVDLRFDDSSGSVFVADGE
                     STSSIDYSMDAAVADLDRSVLQAAGSPSNPALDDFLALPADFPDEAVDRARSITAGLG
                     AYDSAIALEDYFRGDGFRYDRSADYRGEADPLMAFLDNGSGFCQQFATAFAAMARSIG
                     LPSRVAVGFTYGDGAETVDPATGEATTTWTVRGRFAHAWPEVYVNGVGWVAFEPTPGR
                     GNPDARSYSDVPPAQSDELGNSVEFGATTTTTTTIAPAPNGAVPTTLPDQLPPRPGDA
                     AEGTSEEPTSNTPTVVILVIAALAALAVLLVRLRVVAVRRRRVRHRRADVSTADRVSS
                     SWAQTCRDLARVDVRANPSETPVEFATRAAKLVEVEGLVVLGHRESDRRFRATPPDEQ
                     EAASAEQISDEVRDVVWSRLDRQQRLRADLDVG"
     gene            complement(4706..5083)
                     /locus_tag="PKY13_12625"
     CDS             complement(4706..5083)
                     /locus_tag="PKY13_12625"
                     /inference="COORDINATES: protein motif:HMM:NF022684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HOT80830.1"
                     /translation="MPLSEDEQRILTEIEHQLRASDPDLARQVSETTVYSAPLRRTRL
                     AVAGVVVGLAVTILLLLYVNAYVAFVVGFGLMFASAFYLERNIRHLGRVGLNQLATTV
                     RSTGVRDMFGTNAGRDETADPDS"
BASE COUNT          728 a         1993 c         1880 g          816 t
ORIGIN      
        1 tcccgtcgat cgatgctgcc gtcggcgccc acgagggcgg cggcgagctt cgtgccgccg
       61 acgtcgaggg cgagcacaag cggaggaggg accatcgccg aacgatatcg accccgatcg
      121 gctgctgagc gcctcagcgg tcgacaacat gacaggattc ctgtccgatg agcgcgaacc
      181 tgagagccga cgacgtgggt gcgtcaccgt cacagccgtc cttccagcag ctgttcgggg
      241 tgatctgcga caacattcgc cgggtcatcc ggggcaagga ctcggtggtc gagctggcgg
      301 tgctgtgcat gatcgccgag gggcacctgc tcatcgagga cgttcccggc gtcggcaaga
      361 cctcgctggc caaggccctc gctgccaccg tcggcggctc gttcggacgc atccagttca
      421 cccccgacct gctgccgacc gacgtgttgg gcacgtcggt ctggaaccag cagatggggc
      481 acttcgagtt ccgcgccggc ccggtgttca acaacgtggt cctggccgac gaggtcaacc
      541 gctcgtcgcc gaagacccag tcggcgctgc tcgaggcgat ggccgaatcg caggtcacga
      601 gcgacggcgt gacccgcggg ttgacccgcc cgttcatggt gatcgccacg cagaaccccc
      661 tcgagtacca cggcacctac ccgttgccgg agagccagct cgaccggttc ctcctcaagc
      721 tgtcgatcgg ttacccggcc cgcgccgacg agaccctcat cctgaactcc gagggcgcgg
      781 atccgggcct cgccgcgttg gcgccggtcg tgtcggtcgg ccacatcacg gcaatggccg
      841 accactgccg ccagatccat gtgtcgccga cgctgacggg ctatctgatc gacctggcga
      901 tggccacccg cacccaccgg gcgctcgcgc tcggcatgtc gccccgggcg acgttggggc
      961 tcaagcgggc cgtgcaggtg cgggccgcgg cgcagggccg cgaccacggc accgccgacg
     1021 acgtcaaagc actggccgtg ccggcgctcg cccaccgcct ggtcgtggcg cccgaggccc
     1081 agatccaggg tgtcgacccg acggcggtac tcaacgagat cctcggaacg gtcgccgttc
     1141 cgcgcgatgc cggttgagtc gagggcccgg ttcggcctga cccgttcggg caaggccgcc
     1201 gcagtcggcg gtgccgtcac gctcgtcgcg gggcgcgtcg ccggcatcga ggagctgtcg
     1261 gtcgccggcg tctgtctgct cgtcctggtc gcggcgtcgc tcctggtcgt ccgcgccgcc
     1321 ggatccgacc tcgcggtgcg ccgcaccgtt cggccccgtc gcgtcgaggt cggggaccag
     1381 ttcaccgtgt ccctgcagat ccacaacggg cgctcgggcc tgcggttgcc gctcgtcgtc
     1441 ggtgactcgc tcgacggccg ccgggtcgtg cagctctcgt tgccccaacg ccgcaaggac
     1501 ccggcgtgcg tcgcggagta caccgtcggg gcgagtcgcc gtggtctggc cacgttcggt
     1561 ccggtgacga tccgatcgct cgacccgttc gcgttcgcgc gaaccgatct cgtcgtccct
     1621 gcgctgatcg acgtcatcgt cctgccacag atccatcgcc tcgggttgag cggggcggtg
     1681 gcggccgacg acccgagtgg cggcctccgt caccgccgcc agatcgcgac cgtgagcgag
     1741 gagttcgaca cgctgcgcga gtacgtgccc ggcgacgacg tccgtcgggt gcactgggca
     1801 tcgaccgcac ggctcgggcg gccgatggtg cggacccacc agcagccgtc ccaacggcgg
     1861 accacggtgg tcctcgacga tcgggccgac gcctacccgc ccagccgggg cacggccccc
     1921 ttcgagcgag cgatcagcgc cgcggcgtcg gcgctcgtcg cctgccgcga gcagggcgat
     1981 ctcgtgcgcc tcgtgtgcgc cggagggtcc gactccgact acttcgacga cccgcagcgt
     2041 atggacaccg tgctcgacca gctcgcggcg gcgtcgacgt ccgccgtcgg gagcctgcgg
     2101 ggagcgctgg ccaccgccac agcggtcggc tcgggcgacc gcgtggtcgt gtgcaccggc
     2161 acgatcgggg ccgacgaccg cgctgcgatc tcggggtcgg cctcgcgtgg cggtgaacac
     2221 gtggccatca ccaccggcga cggggcgccg gcaacatcgg aggccaaggt cctgaacgtg
     2281 agcttcggcg agggcgacga tctggaccgc cgctggcagg ccgcggtccg ggcccgtgcc
     2341 gccggtccgg tgagggtggg ggcatgaccg ccctggcgcc ccctccccca ccacccccgc
     2401 cggcacccgc accggcggag ccgaccacgg catcgcaggt acggcgcggt accccgaacc
     2461 tcgggctcgg cgccggggtc gacgtcgcgc tcttcgcggt gacggccgcc gtggccgttg
     2521 ccctcgtccg cgccttccgg agctgggatc agctctggcc actcctggcg gtcgtggcca
     2581 tcgcccacgg gttgtcggtc ctcgggcgcg ccctgcgcct cggcgccacg ctcgcgacgg
     2641 cgctgctgct catctccggg atcgtggtca tcacgctcgt ctactaccgg gcacagagtt
     2701 ggtacgggct cccgagcgcg gaggtgttcc gccaggcgtt cgacgacgcc cacgttgcgt
     2761 ttgggccgtt ccagaagctg gtggccccgg tcgacgacct gcccggcttc accgtgacgt
     2821 tcgccgccgg cctgtggttg atggccgtgt tcgccgacct cgccgccggt cggtccgacg
     2881 cgccgctgca ggccgtcgca ccgcacgtcg ccacgttcgt gttctcgtcg gtgctgttgt
     2941 tgggtcgcca cgcactcctc tcgacggccg tcttcgtgac agcgctcgcc ctctatcgcc
     3001 tcgccgtgcg ggccgctcgg ctgcgcgtcg ccgccggcac gcgcccgggc cagcgagtcg
     3061 tctgggtggt gggcgtcccc ctcctcgctg cggtactgct ggcgtcaggt ctcttcgccc
     3121 aggcgtcgcc gaccgactcg ggtggcatgg tcgacctccg ctcgatcggt cggggtccca
     3181 aggcccgggt cgtcgagagc ccgctcgtga gcctcgattc gctgctgcgc ggcgagtcga
     3241 acgacgtgct cttcacggtg acggcgccga ccccgcacta ctggcggctc accgcactcg
     3301 acgacttcga cgggcggagc tggtcggcga gcgcgaagta ccggaacctc gattcgggcg
     3361 agcgcatccc gtccccctgg gacgaggacg tcgcagtgga gcgggagcgg atcgacgtgg
     3421 agatcggcga cctcgagagc gactggttgc cgtccctcta cgcccccacc ggcgtcgacg
     3481 ctcccgtgga cctccgcttc gacgacagtt ccggttcggt cttcgtcgcc gacggcgagt
     3541 cgacctcctc catcgactac tcgatggacg ctgcggtcgc cgacctcgac cgctcggtgc
     3601 tccaggccgc cggctcaccc tcgaacccgg cgctcgacga cttcctcgcc ctgcctgccg
     3661 atttccccga cgaggccgtg gaccgggcgc ggagcatcac tgccggcctc ggcgcgtacg
     3721 actcggcgat cgcgctcgag gactacttcc ggggcgacgg cttccgctac gaccgctcgg
     3781 ccgactaccg cggcgaggcg gatccgctca tggcgttcct cgacaacggc agcgggttct
     3841 gccagcagtt cgccacggcg ttcgcagcga tggcccggtc gatcggcctg ccgtcgcggg
     3901 tcgcggtcgg attcacctac ggcgacggcg ccgagaccgt cgacccggcg acgggcgagg
     3961 ccacgacgac ctggacggtg cggggccgtt tcgcccacgc ctggcccgag gtctacgtga
     4021 acggcgtcgg gtgggtcgcg ttcgaaccga caccgggccg cggcaacccc gatgcccgca
     4081 gctactccga cgtaccgccc gcccagtccg acgaactcgg caactccgtc gagttcggtg
     4141 ccacgaccac cacgacgacc accatcgccc cggcccccaa cggagccgtg ccgacgacgc
     4201 tgcccgatca gctgccgccc cggcccggcg atgccgctga gggcacgtcg gaggagccca
     4261 cgtccaacac ccccaccgtg gtcatcctgg tcatcgccgc gctggcggcc ctggcagtgc
     4321 tgctggtgcg gctccgggtc gtggcggtcc ggcgacgtcg ggtgcgccac cgccgcgccg
     4381 acgtgtcgac cgccgatcgg gtgtcgtcga gctgggcaca gacgtgtcgc gatctggccc
     4441 gtgtcgacgt gcgcgcgaac ccgtcggaga cccccgtcga gttcgccacg cgcgccgcga
     4501 agctcgtcga ggtggagggt ctcgtggtcc tcggccaccg ggagtcggac cggcgcttcc
     4561 gagcgacgcc tcccgacgaa caggaagccg cgtcggccga acagatcagc gacgaggtgc
     4621 gcgacgtcgt gtggtcacga ctcgaccgcc agcagcggct tcgagccgat ctcgacgtcg
     4681 gctgaaccga cgccgcagcg gccgttcagg aatcggggtc ggcggtctcg tcgcggccgg
     4741 cgttggtgcc gaacatgtcg cggacgccgg tggaccgcac cgtggtggcg agctggttga
     4801 gcccgacgcg accgaggtga cggatgttgc gctcgaggta gaacgccgac gcgaacatca
     4861 gcccgaaccc gacgacgaac gccacgtagg cgttgacgta gagcagcagg aggatggtga
     4921 cggccaaccc gacgacaacc ccggccacgg caaggcgcgt ccgccgcagc ggcgccgagt
     4981 acaccgtcgt ctcgctcacc tggcgggcga gatcgggatc cgacgcgcgg agctggtgct
     5041 cgatctcggt gaggattcgt tgttcgtcct cggacagcgg caccgcgggt cccccggaag
     5101 gattgagcac cggccctgcc cagcgggcga cggacggcgt gggtggatgg ctcggttggt
     5161 tccgtcatga tcgcacagcg atcccggaac gacaacgcgc accgcttcat ttggtgcgga
     5221 tccgcccgac cggcccgaac gcactggttg agacctgaac gacgtcgatg cccccgcaga
     5281 ctccgcgata ggttcacccg cccgctgacc ccgaccagga gtacgaacca tgacacgacg
     5341 attcagcaag gttgccgcga tcacggcgat cgccagcgcg cccttctccc tgacggcctg
     5401 cacgccgtgc cccggcg
//
LOCUS       DAOSOA010000228         5363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15388_length_5363_cov_6.683873, whole genome shotgun sequence.
ACCESSION   DAOSOA010000228 DAOSOA010000000
VERSION     DAOSOA010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15388_length_5363_cov_6.683873"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..630
                     /locus_tag="PKY13_12630"
     CDS             178..630
                     /locus_tag="PKY13_12630"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HOT80831.1"
                     /translation="MRIVTDDPSGMSEKFEILAAYSDAMFSGDTAAVLEYWSEDFHSN
                     VTTRVSPEQVGTDVRGQEQLYWQQARSAFPDMEFSVNLLIEADDLVISNWTITGTHTG
                     TAFFDVAPSGEPVEINGTAVLRFRDGKIVEHWGGPHCQAGLGLLPVSR"
     gene            complement(620..1663)
                     /locus_tag="PKY13_12635"
     CDS             complement(620..1663)
                     /locus_tag="PKY13_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80832.1"
                     /translation="MPWGSRRRRAIAVAGLAIVGVVAGCGDDTATSDEADPTGESTST
                     TVSAPSTTIAGGRLAIVDFRNFTYDFNGAPPDGSDPAAVASPVAVVGGSFSDPEGSDD
                     PLSAGRRFFVVTDVDYVDLDGDGEDEAAVSVLAGYSNSPARDTDVLVYRATDEGPAFV
                     TDIGWSDREDGAVHDVAASTGSAGERLVVQLVEPIDEECCDHGIVERSFRLSGDRLAE
                     DGDPTRWPVVGLEGAGPEPVAVSFLPGSQRASIGGVLTNEPRPVTFDAVEGQVLSVRA
                     RAVEAGDVPFTVTITGPDTTVRSVAAGETVDAGLELTVSGTYVIAVVLDGPVADESPD
                     TGQRFWLDFVLSD"
     gene            complement(1693..2715)
                     /locus_tag="PKY13_12640"
     CDS             complement(1693..2715)
                     /locus_tag="PKY13_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80833.1"
                     /translation="MRHRRASRLVLVAWLATAVLLGSACSSDDDATPNATATTTSEPA
                     DPAGPSTTAGPGAASIAEVDFRNFTYEYPGVGPDDELSEVTVVDGEFSEGEESGDSFY
                     FEVVDVDLADLDGDGVDEAAITNYYSTGGSGQFTDVLIYRWTGTTAEYVTDAGVGDRG
                     DGGVDDVSVAPAGGVEGDVLVVRRNADAEGACCPTALEERTLRLRDDELIEIGEPEKW
                     GIVRVGVDADGNPTDAPTEVKFLAGTSRADLTGDATTPVAAEFDVTAGQTLDLALDAA
                     ALSENPLVVVISGPDGEAGRIGTGVDPSISLPLPTSGTYRLQFDPVPPVAEDLGVYFD
                     ADLRIL"
     gene            complement(2715..3539)
                     /locus_tag="PKY13_12645"
     CDS             complement(2715..3539)
                     /locus_tag="PKY13_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOT80834.1"
                     /translation="MADDDLRDFERDFFTHDDVSRTIYRKGSGPAVIVMAEIPGITPH
                     VADFARKVADIGCTAVLPHLFGTPGRDIDPATTSRLSTTLYGLSSIVPACISKEFTVF
                     ATGASSPVVTWLRALAKHEHERCGGPGVGAVGMCFTGGFALAMAADDRMLAPVLSQPS
                     LPVPIGRKRAHNIDISPDDLRIVKDRCAKDGLEVLGLRFKSDKLVGGERFAFLKEQLG
                     DAFVAVELEDADANPAAAMPPHSVLTEHLIDEPGQPTRAALDQVLELFRSRLLTDA"
     gene            3636..3959
                     /locus_tag="PKY13_12650"
     CDS             3636..3959
                     /locus_tag="PKY13_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80835.1"
                     /translation="MSRLIPGLPTDVFKKVDDLIADVKPILVEVDTTLTTVSTTLTDV
                     DVTLGQATATLTEATAVLTDVKGLLGDLETKLELLDEVPAMKQQLAEVHAMLSGLTAG
                     ARSKK"
     gene            complement(4030..4506)
                     /locus_tag="PKY13_12655"
     CDS             complement(4030..4506)
                     /locus_tag="PKY13_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT80836.1"
                     /translation="MDWIEVDTTTCPVAMTLGLLGEKWTLLIVRDALNGVRRFDEFRR
                     HIGLSEAVLSDRLQKLVAAGVLATRPYREEGSRPRNEYRLTDRGWDLLPVIVALKQWG
                     EKHLADRDQPVLQIAHRDCGGDVRAELVCADHPGTVLGVHETRAVVGPGARAHTAV"
     gene            4594..5046
                     /locus_tag="PKY13_12660"
     CDS             4594..5046
                     /locus_tag="PKY13_12660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOT80837.1"
                     /translation="MTDAAPTVSPHLLSQEEFDVLDLDSSSAFMRGAGLIVTEVTGTS
                     VVGHIDLTREHHTPWGIVHGGVYATAIESAASIGATAAVRDRNMVAVGLTNTTQFLRS
                     LTDGRVDVAATALHQGRSQQLWKVDVTDGRGRLVAHGELRLQNLDIPA"
     gene            5059..>5363
                     /locus_tag="PKY13_12665"
     CDS             5059..>5363
                     /locus_tag="PKY13_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT80838.1"
                     /translation="MDALRTPDDRFAGLPGYDFAPHYAHVGSASGEALRLHYLDEFPA
                     AGMPGAGSTIVLLHGEPSWSFLYRKMIPVLTAAGHRCIAPDLIGFGRSDKPTELDDYT
                     "
BASE COUNT          841 a         1913 c         1829 g          780 t
ORIGIN      
        1 cagctcgtcg tcgacggggc gtggaccgat cccgacctcg accaccgccg cttcgaggcc
       61 acgcgccgcc ttcgctgacc ggcgggtcct ccggaccccg ccggaccggg tggaggatgg
      121 tgcgccagcg gtccggtttc ggcgcgctgg cgcaccatcc tcggtagctt cggccgtatg
      181 cgaatcgtga ccgacgatcc ctccggcatg tccgagaagt tcgagatcct cgcggcgtac
      241 agcgacgcga tgttctccgg cgacaccgct gcggtgctcg agtactggtc ggaggacttc
      301 cactccaacg tgacgactcg ggtctcgccc gagcaggtcg gcaccgacgt gcgcggccag
      361 gaacagctct actggcagca ggcacggtcg gcgtttcccg acatggagtt cagcgtcaac
      421 ctgctcatcg aggccgacga cctggtcatc tccaactgga cgatcaccgg cacgcacacc
      481 ggcaccgcgt tcttcgacgt ggcgccgtcg ggcgagccgg tcgagatcaa cggcacggcc
      541 gtgctccggt tccgcgacgg caagatcgtc gagcactggg gtggaccgca ctgccaggcc
      601 gggctggggc tcctgcccgt cagtcgctga gcacgaagtc gagccagaat cgctgaccgg
      661 tgtcgggcga ctcgtcggcg acgggcccgt cgaggaccac cgcgatgaca taggtgccgc
      721 tgaccgtcag ctcgaggccg gcgtcgacgg tctcgccggc ggcgaccgac ctgaccgtcg
      781 tgtcggggcc ggtgatcgtc accgtgaagg ggacgtcgcc ggcctcgacc gcacgagcgc
      841 gaaccgacag cacctgccct tcgaccgcgt cgaacgtcac gggccggggt tcgttcgtca
      901 gcaccccacc gatgctggcg cgctggctgc ccgggaggaa cgacacggcg acgggctcgg
      961 ggcccgcgcc ctccaacccg accaccggcc agcgggtggg atcaccgtcc tccgccagcc
     1021 ggtcgcccga cagccggaag gagcgctcga cgatcccgtg gtcacagcac tcctcgtcga
     1081 tgggttcgac cagctgcacg accaggcgtt caccggcgga cccggtggac gcggccacgt
     1141 cgtgcacggc gccgtcctcg cgatcgctcc acccgatgtc ggtcacgaag gccggcccct
     1201 cgtcggtggc gcggtagacg agcacatcgg tgtcgcgggc gggcgagttg ctgtacccgg
     1261 ccagcacact gacggccgcc tcgtcctccc cgtctccgtc gagatcgacg tagtcgacgt
     1321 cggtcacgac gaagaaccgt cgaccggcgc tgagcgggtc gtcggagccc tccggatcgg
     1381 agaagctgcc accgacgacg gcgaccggcg atgccacggc tgcggggtcg gagccatcgg
     1441 gtggagcgcc gttgaagtcg tacgtgaagt tgcgaaagtc gacgatggcg agacggccac
     1501 cggcgatcgt ggtcgacggg gcactgacgg tcgtcgatgt cgactccccg gtcgggtccg
     1561 cctcgtcgga ggtggccgtg tcgtcgccgc aacctgcgac gactccgacg atggccagcc
     1621 ccgcgacggc gatcgcacga cggcgacgcg aaccccaggg catccgccga gggtacttcc
     1681 ggtgaacgaa cgtcagagaa tccgcaggtc ggcgtcgaag tacacgccga ggtcctcggc
     1741 gaccggcggc accgggtcga actgcaggcg gtaggtaccc gacgtgggca acggcaacga
     1801 gatcgacggg tcgacaccgg tgccgatccg gccggcctcg ccgtcgggac cactgatcac
     1861 gaccacgagc gggttctcgg agagcgccgc cgcgtcgagc gcgagatcga gggtctggcc
     1921 cgctgtcacg tcgaactccg ctgcgaccgg tgtggtggca tcgccggtca ggtccgcccg
     1981 gctcgtgccg gcgaggaact tcacctcggt cggagcgtcg gtggggttgc cgtcggcatc
     2041 gacgcccacg cggacgatgc cccacttctc gggctcgccg atctcgatga gctcgtcgtc
     2101 gcggagccgc agggtgcgct cctcgagcgc cgtgggacag cacgcccctt cggcgtccgc
     2161 gttgcgccgc acgacgagca cgtcgccctc gactccaccg gcgggggcaa cgctcacatc
     2221 gtcgacgcca ccgtcgccgc ggtcacccac gccggcgtcg gtgacgtact cggcggtcgt
     2281 gccggtccag cggtagatca gcacgtcggt gaactgaccg gatccgccgg tgctgtaata
     2341 gttcgtgatg gccgcttcgt cgacgccgtc gccgtcgagg tcggcaaggt cgacgtcgac
     2401 gacctcgaag tagaaggagt cgcccgactc ttcaccctcg ctgaactcac cgtcgacgac
     2461 cgtcacctcc gacagctcgt cgtcgggccc cacgcccgga tactcgtagg tgaagttccg
     2521 gaagtcgacc tccgcgatcg acgcggcgcc ggggcctgcc gtcgtcgacg gtccggccgg
     2581 gtcggccggc tcgctcgttg tggtggccgt cgcgttcggg gtggcatcgt cgtccgacga
     2641 acaggccgag cccaggagca cggcggtcgc gagccacgcg acgaggacga gccggctcgc
     2701 cctgcggtgc cgcatcacgc gtcggtcagg agtcgcgaac ggaacagttc gagcacctgg
     2761 tccagcgcgg cccgggtggg ttgccccggc tcgtcgatga ggtgctcggt gagcaccgag
     2821 tgcgggggca tcgccgccgc cgggttcgca tcggcgtcct cgagttcgac cgcgacgaac
     2881 gcgtcgccga gttgctcctt caggaacgcg aaccgctcgc cgccgacgag tttgtcgctc
     2941 ttgaagcgga ggccgagcac ctcgagcccg tccttggcac agcggtcctt gacgatgcgc
     3001 agatcgtcgg ggctgatgtc gatgttgtgg gcccgcttgc gaccgatcgg gaccggcagc
     3061 gagggctgag agagcactgg tgcgagcatg cggtcgtccg ccgccatcgc cagcgcgaac
     3121 ccgccggtga agcacatgcc caccgcaccg acgcccggcc cgccgcaccg ttcgtgctcg
     3181 tgcttcgcga gggcccgcaa ccacgtgacc acgggcgacg acgctccggt cgcgaacacc
     3241 gtgaactcct tgctgatgca cgccgggacg atcgacgaca acccgtagag cgtggtcgac
     3301 aggcggctgg tggtggccgg gtcgatgtcg cgaccggggg tgccgaacag gtggggcagg
     3361 accgcggtgc acccgatgtc ggcgaccttg cgggcgaagt ccgcgacgtg gggcgtgatg
     3421 ccggggatct ccgccatcac gatgaccgct gggcccgagc ccttgcggta gatcgtgcga
     3481 ctgacgtcgt cgtgggtgaa gaagtcgcgc tcgaagtcgc ggagatcgtc gtcggccatc
     3541 gccagaccgt atccgtgccg agcggggagc ggcgtgaacg ccggaccgac cccgttctcc
     3601 tgacctgagg tcaacagcgc cgctagcctc ggcacatgtc gcgactcatc ccgggcctgc
     3661 ccaccgacgt gttcaagaag gtcgacgatc tcatcgccga cgtgaagccg atcctcgtcg
     3721 aggtcgacac gacgctcacc acggtcagca cgacgctcac cgacgtcgac gtgaccctcg
     3781 gtcaggcgac cgccacgctc accgaggcga cggcggtgct caccgacgtg aagggcctgc
     3841 tcggtgatct cgaaacgaag ctcgaactcc tcgacgaggt cccggcgatg aagcagcagt
     3901 tggccgaggt gcacgcgatg ctctccggtc tcacggccgg agcccggtcg aagaagtaga
     3961 ccgccgaccg ggaagaacgc gccgccagcg acgcggaccg aactgacgcg cagcaggacg
     4021 gatcggcgct cagaccgcgg tgtgagcgcg ggcgccaggg ccgacgacgg cgcgcgtctc
     4081 gtggaccccg agcaccgttc caggatgatc agcacagacc aattcggcgc ggacgtcacc
     4141 gccgcagtcc cgatgggcga tctgcagcac cggctggtcg cggtccgcca ggtgcttctc
     4201 gccccactgc ttcagggcga cgatcaccgg caggaggtcc cagccccgat cggtcaggcg
     4261 gtactcgttg cgcggacggc tgccctcctc ccggtacggc cgggtcgcca gcaccccagc
     4321 ggcgacgagc ttctgcagtc ggtccgagag caccgcctcg gacaacccga tatggcgtcg
     4381 gaactcgtcg aaccggcgca cgccgttgag cgcgtctcgg acgatcagga gggtccactt
     4441 ctccccgagg aggcccaggg tcatcgccac ggggcacgtc gtggtgtcga cctcgatcca
     4501 gtccatggac tgcagcgtac cctgacttct tcttgcgtag ccagggtggc gactggttga
     4561 cttcgaactc cgcagccagc ccgaggagcc cccatgaccg acgccgcgcc caccgtgtcc
     4621 ccgcacctgc tctcccagga ggagttcgac gtgctcgacc tcgactcgtc atccgccttc
     4681 atgcgtggcg ccggtctgat cgtcaccgag gtgacgggca catcggtggt gggccacatc
     4741 gacctcaccc gcgagcacca cacgccctgg ggcatcgtcc acggcggggt gtacgcgacc
     4801 gccatcgaga gcgctgcgag catcggcgcg acggcggccg tgcgcgaccg caacatggtc
     4861 gccgtcggcc tcaccaacac gacccagttc ctgcgctcgc tcaccgacgg tcgggtcgac
     4921 gtcgcggcga cggcgctcca ccagggacgc agccaacagc tctggaaggt cgacgtcacc
     4981 gacgggcggg gccgcctcgt cgcccacggc gaactgcgcc tccagaacct cgacatcccg
     5041 gcctgacaga ctccgaccat ggacgcactc cgcacccccg acgaccgctt cgccggactc
     5101 cccggctacg acttcgcgcc tcactacgcc catgtcggca gcgccagcgg cgaggccctg
     5161 cgtctgcact acctcgacga gttcccggcc gcgggaatgc ccggcgccgg ctcgacgatc
     5221 gtgctgctgc acggcgaacc gtcgtggtcg ttcctgtacc gcaagatgat cccggtgctg
     5281 acggccgcgg gacatcgctg catcgcaccc gacctcatcg ggttcggccg ctccgacaag
     5341 ccgaccgaac tcgacgacta cac
//
LOCUS       DAOSOA010000229         5337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15495_length_5337_cov_5.601287, whole genome shotgun sequence.
ACCESSION   DAOSOA010000229 DAOSOA010000000
VERSION     DAOSOA010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15495_length_5337_cov_5.601287"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PKY13_12670"
     CDS             <1..765
                     /locus_tag="PKY13_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOT80839.1"
                     /translation="AWEVQARGELQLAILIEMMRRDGFELTVGKPQVVTKEIDGHLSE
                     PVERLTIDVPEEHLGAVTSLVAPRKGRLENMTNHGTGWVRMDYLVPARGLIGFRTEFL
                     TETRGTGIMHHVFETYEPWAGELRARERGSIVSDRRGSTTAYAITALQERCTLFVPPG
                     TEVYEGMIVGENSRSDDMDVNICREKKLTNMRTSSSDHTVPLVPPRLLSLEQALEFIE
                     SDECVEVTPNFVRLRKVELDATDRAKSLKQLKNARS"
     gene            806..1579
                     /locus_tag="PKY13_12675"
     CDS             806..1579
                     /locus_tag="PKY13_12675"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT80840.1"
                     /translation="MPPQRSDSLRTQAVLISTAERLFAERGLEAVSLSEINRDVGQRN
                     KSALHYHFGSRDGLLKALLEKHRLRLDQERMAMLEDYGAADELSLHDAVSIMVRPLAR
                     RLGDHEDGGVHYIRIMAQLSATPGHPLNDWLFNHLPPAFAAIAPVLYRHIPRAPTLLR
                     MRRTQLMNGTMFHALLLETYSSPDDFPSPEEHRAQQELYINDLIDSICGMLSAPLSPL
                     SEAVLAQRAEERVALPATDDELPVFGDGKERGLLTFEGD"
     gene            1612..2271
                     /gene="nucS"
                     /locus_tag="PKY13_12680"
     CDS             1612..2271
                     /gene="nucS"
                     /locus_tag="PKY13_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HOT80841.1"
                     /translation="MRLVIARCSVDYAGRLTAHLPLATRLLMVKADGCVAIHADGGAY
                     KPLNWMNAPNRLIEGDGVWTVTNPKGETLTITLEEVLHDSAHELGIDPGLQKDGVEAH
                     LQELLAANTEAIAQGLVLVRREFPTDIGPVDLLCRDADGTAVAIEVKRRGEIDGVEQL
                     TRYLERLERDPTLRGVRGVFVAQSIKPQAKVLAASRDIACVEVDYDELRGIESNILKL
                     F"
     gene            complement(2258..3142)
                     /locus_tag="PKY13_12685"
     CDS             complement(2258..3142)
                     /locus_tag="PKY13_12685"
                     /inference="COORDINATES: protein motif:HMM:NF023354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HOT80842.1"
                     /translation="MAGSYFPLDDRPFRLRMGLRPLDLAEWLEPDEQAADDLAMKQQL
                     VAERYDDVVAIVDEPNVHAACDELWSLVEAATRGFVLRGTTPASLLAAQNPDDHHPIV
                     RAGLATQEDWAVMAPVGGRLVLAAACVCFPTRWVLATRIGKPMAAVHEHVAYYDEHLS
                     RPVDSFFERLTVDRPVWRLNWNLMDDPTLFQPVAGPAASVAPDEVGDRIWLRIERQTL
                     RRLPETGAIVFGIRIHQRPLSALVARPDQLATLRTAIANLPPETFAYKSLGGFADALD
                     TWIGARTTNVRAGPIRRA"
     gene            complement(3151..3996)
                     /locus_tag="PKY13_12690"
     CDS             complement(3151..3996)
                     /locus_tag="PKY13_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOT80843.1"
                     /translation="MPINPDAVGVTSDPSTTSWRSKDSLLYALGVGAGVDDLAFTTEN
                     SMNVTQRALPTQVVTIADVSGVFGNIGSFNPAMLVHGEQRITLHRELPVEGTVSTVAE
                     ITGIYDKVKGAVIEITARSTDAASGEPLFDSVMSAFIRGEGGWGGDRGPSGPQNAAPD
                     RDADEVVTLQTTRDQALIYRLSGDRNPLHSDPGFAALAGFDRPILHGLCTYGFTGRAV
                     LGALCDNDPARFRHMEGRFSSPVFPGEALTVRIWRTGDGEAVFRTEGGDGRTVIDSGL
                     ARFDV"
     gene            4225..>5337
                     /locus_tag="PKY13_12695"
     CDS             4225..>5337
                     /locus_tag="PKY13_12695"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOT80844.1"
                     /translation="MTVASNVTLAGNDVGGESGDRLDASLRELDESFATTPVRIVTPD
                     FAIDTTAGDLGLAVDVGATKEAALEVGRNDPGPLAPLRWAKSFVSERVAPVTLRVDRQ
                     TASAAIVAAEGDRRTKPVEPTMDVSPEKIDMTPGKPGKALDVDDILAQAPNGITEVGG
                     TITIETTQQETRPKVSDGEVLAVIARANNVTSHPLTLKYGETTSEIKGDDLRAGFRLD
                     TSGSTAKLGLDDDYVATLLAERLKVPANPTGVRFDIVNGVPTPVPGTDAVVCCDAGAP
                     ATIVAALLDGKSEVTLGTRTVTAAEGVQWANTLGVKEVVGEFTTQHPCCAPRVTNIHK
                     MSDLTKGIIIAPGETFSANDTVGRRTKEKGFVSAPVI"
BASE COUNT          917 a         1880 c         1749 g          791 t
ORIGIN      
        1 gcctgggagg ttcaggcccg cggcgagctg cagctggcga tcctcatcga gatgatgcgc
       61 cgcgacggct tcgagctgac ggtcggcaag ccgcaggtcg tgaccaagga gatcgacggc
      121 cacctgtccg agccggtcga gcggctcacg atcgacgtcc ccgaggagca cctcggcgcc
      181 gtcacgtcgc tcgtcgcccc ccgcaagggt cgcctcgaga acatgaccaa ccacggcacc
      241 ggctgggtcc gcatggacta cctggtgccc gcccgcggcc tcatcggatt ccgcaccgag
      301 ttcctcaccg agacgcgtgg caccggcatc atgcaccacg tgttcgagac ctacgagccg
      361 tgggccggcg agctccgtgc ccgtgagcgc ggctcgatcg tgtccgatcg acgtggttcg
      421 accaccgcgt acgccatcac tgcgctccag gagcgttgca cgctcttcgt gccgccgggc
      481 accgaggtgt acgaaggcat gatcgtgggc gagaactccc gctccgacga catggatgtc
      541 aacatctgcc gtgagaagaa gttgacgaac atgcgcacgt cgtcctcgga ccacaccgtg
      601 cccctggttc cgccccgtct cttgtcgctc gagcaggcgt tggagttcat cgaatccgac
      661 gaatgcgtcg aggtgacgcc gaacttcgtg cggctgcgca aggtcgaatt ggatgcaacc
      721 gaccgcgcga agtcgctgaa acagttgaag aacgcacggt cctaacttag gttggttcac
      781 gaccaaacca gttcgtgagc cgcccatgcc accacaacga tccgacagcc tgcgcacgca
      841 ggctgtcctc atctcgaccg ccgagcgact gttcgcggaa cgtgggctcg aggcggtgtc
      901 actcagcgag atcaaccgcg acgtgggcca gcgcaacaag tcggcgctgc actaccactt
      961 cgggagtcgt gacggtctcc tgaaggcgct gctcgagaag catcgcctcc gcctcgacca
     1021 ggagcggatg gcgatgctcg aggactacgg cgccgccgac gagctgagcc tgcacgacgc
     1081 cgtgagcatc atggtgcgcc cgctggcgcg ccgattgggt gatcacgagg acgggggcgt
     1141 ccactacatc cggatcatgg cgcagctgtc cgcaacgccg ggacacccgc tcaacgactg
     1201 gctcttcaac cacctcccgc cggccttcgc agccattgcg ccggtgctct atcgacatat
     1261 tccccgcgcc cccaccttgc ttcgcatgcg tcgcacacaa ctcatgaacg gaacgatgtt
     1321 ccatgcgttg ctgctcgaga cgtattcctc gcccgacgat ttcccctccc ccgaggaaca
     1381 tcgggcgcaa caggagctct acatcaatga tctgatcgat tcgatctgcg ggatgttgag
     1441 cgctcccctg tccccactca gcgaggccgt gttggcacaa cgcgccgagg aacgcgtggc
     1501 gttgccggcg accgacgacg agctgcccgt cttcggcgac ggcaaggaac gcggcctcct
     1561 caccttcgaa ggcgactgag ccgacgccgc ggtcccgtag gatcacggcc cgtgcgtctc
     1621 gtgattgccc gctgctccgt cgactacgcc ggtcgcctca ccgcccacct cccgctcgcc
     1681 acgcggctcc tcatggtgaa ggccgacggg tgcgtcgcca tccacgccga cggcggggcc
     1741 tacaagccgc tgaactggat gaacgccccc aaccgcctga tcgagggcga cggggtgtgg
     1801 acggtcacca accccaaggg cgagaccctc acgatcacgc tcgaggaggt cctgcacgac
     1861 agcgcccacg agctcggcat cgaccccggc ctccagaagg acggcgtcga ggcccatctc
     1921 caggagctgc tcgcggccaa caccgaggcg atcgcacaag gcctcgtgct ggtccgacgg
     1981 gagtttccga ccgacatcgg tccggtcgac ctcctgtgcc gcgacgccga cgggaccgcc
     2041 gtcgccatcg aggtgaagcg gcggggagag atcgacgggg tcgagcagct gacgcgctac
     2101 ctcgaacgcc tcgaacgaga tccgacactc cgtggagtac gcggcgtgtt cgtcgcccag
     2161 agcatcaagc cccaggccaa ggtcctcgcc gccagtcgcg acatcgcctg cgtggaggtc
     2221 gactacgacg agctccgcgg catcgaatcc aacatcctca agctcttctg atgggccccg
     2281 cacgaacgtt ggtcgttcgt gcgccgatcc aggtgtccag ggcgtcggcg aacccgccga
     2341 ggctcttgta ggcgaacgtc tcgggcggga ggttggcgat cgcggtgcga agggtcgcca
     2401 gctggtcggg acgggcgacc agcgccgaga gcgggcgttg gtggatccgg atgccgaaca
     2461 cgatggcgcc ggtctcgggg agtcgacgaa gggtctggcg ctcgatgcgg agccagatgc
     2521 ggtcgcccac ctcgtcgggc gccaccgacg ccgccggacc cgccacgggc tggaacagcg
     2581 tcgggtcgtc catcaggttc cagttgagcc gccacaccgg ccggtcgacg gtgagccgct
     2641 cgaagaacga gtcgaccggc cgagacaggt gctcgtcgta gtaggcgacg tgctcgtgca
     2701 ccgcggccat cggcttgccg atcctcgtcg cgagaaccca gcgtgtcggg aagcacacac
     2761 aggccgctgc caacaccagt cggcccccga ccggcgccat caccgcccag tcctcctggg
     2821 tcgccaaccc ggcccgaacg atcgggtgat gatcgtcggg gttttgtgct gcgaggagcg
     2881 acgctggcgt cgtccctcgc agcacaaaac ccctggtggc ggcttcgacc aggctccaga
     2941 gttcgtcgca ggcagcgtgg acgttgggtt cgtcgacgat tgcgacgacg tcgtcgtagc
     3001 gctcggcgac cagctgctgc ttcatcgcga ggtcgtcggc ggcctgctca tcgggttcga
     3061 gccactcggc gaggtccagg ggccgcagtc ccatccggag gcggaacgga cggtcgtcga
     3121 gcgggaagta cgaccccgcc atcggtcgga tcagacgtcg aaacgggcga gcccggagtc
     3181 gatgaccgtg cggccgtcac cgccttcggt ccggaacacg gcctcgccgt cacccgtacg
     3241 ccagatgcgg acggtcaacg cctcaccggg gaagaccggc gagctgaacc gcccctccat
     3301 gtgccggaac cgggcgggat cgttgtcaca cagggcgccg agcacggctc gaccggtgaa
     3361 tccgtaggtg cagaggccgt ggaggatcgg acgatcgaag ccggcgagcg cggcgaaccc
     3421 cgggtcggag tgcaacgggt tgcggtcgcc cgacaggcga tagatcaggg cctggtcacg
     3481 ggtggtctgc agcgtgacca cctcgtcggc gtcgcgatcg ggggcggcgt tctgcggacc
     3541 cgacgggcca cggtcgccac cccagccgcc ctcgccgcgg atgaacgccg acatcaccga
     3601 gtcgaagagc ggttcccccg acgctgcgtc ggtggagcgg gccgtgatct cgatgacggc
     3661 gcccttcacc ttgtcgtaga tgccggtgat ctcggcgacc gtgctgacgg tgccctcgac
     3721 gggcaactcc cggtgcagcg tgatgcgctg ctcgccgtgc acgagcatcg ccgggttgaa
     3781 cgacccgatg ttgccgaaca cgccactgac atccgcgatc gtcacgacct gggtcggcag
     3841 ggcccgctgg gtcacattca tggagttctc ggtcgtgaac gccaggtcgt cgacgccggc
     3901 acccaccccg agggcgtaca acagcgagtc cttggaacgc caggaggtgg tggacgggtc
     3961 ggacgtgacg ccgacggcat caggattgat cggcatcggc cggtgactcc ttgctctcgt
     4021 caccggcgcc atgacgatcc ggtgacacct tcgtgacgat gtgggcaatc aggaggggat
     4081 gccccgacga acccgcgctg agcggccatc ctatggggag tgtcccgatg gaaagtagtc
     4141 ctcatccttt gcctggccgc gcctctggtg gcgttcggcc tcctcgtcgc gctttgggca
     4201 gcgctcggcc ccgacgaaca gccggtgacc gtcgcgtcga acgtcaccct cgccggcaac
     4261 gacgtcggcg gcgagtccgg agaccgactc gacgcgagcc tccgcgagct cgacgagtcg
     4321 ttcgccacga cgccggtccg catcgtcacc cccgacttcg cgatcgacac gaccgccgga
     4381 gatctcggcc tcgccgtcga cgtcggagcg acgaaggagg cggcgctcga ggtcggccgc
     4441 aacgaccccg gaccactcgc tcccctccgg tgggccaagt cgttcgtcag cgaacgcgtc
     4501 gccccggtca cgctccgcgt cgaccgccag accgcgtcgg cggcgatcgt cgctgcggag
     4561 ggcgaccgtc ggaccaaacc ggtcgagccg acgatggacg tcagccccga gaagatcgac
     4621 atgacgccgg gcaaacccgg caaggccctc gacgtcgacg acatcctggc ccaggcgccc
     4681 aacgggatca ccgaggtcgg cggcactatc acgatcgaga cgacccagca ggagacccgc
     4741 ccgaaggtgt ccgacggcga ggtcctcgcc gtcatcgccc gcgccaacaa cgtcacctcc
     4801 catccgctca ccctcaagta cggcgagacg accagcgaga tcaagggcga cgacctccgc
     4861 gccgggttcc gcctcgacac ctccggcagc accgccaagc tcggtctcga cgacgactac
     4921 gtcgccacgc tcctcgccga gcgcctgaaa gttccggcga acccgacggg cgtgcggttc
     4981 gacatcgtca acggcgtccc cacgccggtg cccggcaccg acgccgtcgt gtgctgcgac
     5041 gccggggccc cggcgacgat cgtcgccgcg ctgctcgacg gcaagagcga ggtcacgttg
     5101 ggcacgcgca cggtgacggc ggccgagggc gtccagtggg cgaacaccct cggggtcaag
     5161 gaggtcgtcg gcgagttcac gacccagcac ccgtgctgcg ctccccgcgt cacgaacatc
     5221 cacaagatgt ccgacctcac caagggcatc atcatcgcgc ccggcgagac cttctccgcc
     5281 aacgacaccg tcggccggcg aaccaaggag aaggggttcg tcagcgcgcc cgtcatc
//
LOCUS       DAOSOA010000230         5323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15553_length_5323_cov_4.374905, whole genome shotgun sequence.
ACCESSION   DAOSOA010000230 DAOSOA010000000
VERSION     DAOSOA010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15553_length_5323_cov_4.374905"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PKY13_12700"
     CDS             complement(<1..1275)
                     /locus_tag="PKY13_12700"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT80845.1"
                     /translation="MDVTPQLIEQIDFAEKFRGYDPDQVDDFLERVGATLAELTKTSR
                     DAIARAERAEAELRNAPEAGRAPAAAAAAMSDEEEAMQSTRTLMMAKRTADAAIADAR
                     QEAQKLVADARTAAEQQAREARTEADRIVGEARVNADQMTQKATAEANREYGARRDQI
                     LAEIGELENRKVGMASQLVAIEGRLEEYRSALTSVHGAIGAVLDDPETALRSPLPGID
                     LAGIVRPVAEPVPEPDVAPADVASSDDAPFDAAPYEPAVFAPVADVAPDARSFDTEPS
                     YAEPIGEPAFTDSPFGEPVGSEPAFADAAFSEPAFSDAGFADVAFSDDDPAFRNGSSG
                     SHFGEATLLDPNDLAVPYSEETELAGGADLPWSDDRLDDRADEAPVGDPWGPGSWGEV
                     ASFPDSSAAFAPEGEVYEREATATTASHAFTEF"
     gene            complement(1414..2028)
                     /gene="sepF"
                     /locus_tag="PKY13_12705"
     CDS             complement(1414..2028)
                     /gene="sepF"
                     /locus_tag="PKY13_12705"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOT80846.1"
                     /translation="MAGMLRKGMSWLGLGPDDEYDDYDDYEEDPVVDLTGPHRAVSTG
                     TVNPSPVVRAVPAPSRVEPMPDWEEPVESASVRVLPSAGGRPRGGVVTAPEPVRPRSS
                     VVRPVPAASSASARPHVVTPKTFNDAQEVGDWFRKRQPVIVNLQDLDRDLARRLLDFS
                     SGVAYGLGGSVERVASHVYLLSPIDVEISPDDRRRLRERGLVEE"
     gene            complement(2128..2802)
                     /locus_tag="PKY13_12710"
     CDS             complement(2128..2802)
                     /locus_tag="PKY13_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HOT80847.1"
                     /translation="MSIVPDPDLVDRLAARRAAIEERIRVASGAPSFAESGVRLIAVS
                     KTHPAETIVAAAAAGFVEFGESYAVEFAAKAPEVGAAVRWHFIGQLQTNKVRVVAPWT
                     DVYQSVDRPSLVAELAKRVSGATVMVQVNLADVEGRGGVSWQDAPGLIESARAAGLSV
                     VGAMGVAPLADDATVAAAFGRLRSLCDAEALPECSIGMSGDLEIAIAEGSTMVRVGTD
                     IFGSRP"
     gene            complement(2816..>3213)
                     /locus_tag="PKY13_12715"
     CDS             complement(2816..>3213)
                     /locus_tag="PKY13_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOT80848.1"
                     /translation="IGAAHAGWKGLYEGVLEATVGALIDLGARDISARLGPCISPAAY
                     EFSERDLTTMALRFGPEVVGATEGGDAALDMTAAVRSALRSVGIDDVDVAAAACTATT
                     IGADGTPRFFSHRARADTGRQASVIWIEP"
     assembly_gap    3214..3313
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3314..3657)
                     /locus_tag="PKY13_12720"
     CDS             complement(<3314..3657)
                     /locus_tag="PKY13_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HOT80849.1"
                     /translation="MAVQHRFSERADGDFAITAEPRALAAARSALAPHPWTWLEQVHG
                     AGVVVVSRPRQWAGARADAAVTSAPGAVLAVQTADCAPVLFTAVGPGPSAGPVIGAAH
                     AGWKGLYEGVLEA"
     gene            complement(3667..4806)
                     /gene="ftsZ"
                     /locus_tag="PKY13_12725"
     CDS             complement(3667..4806)
                     /gene="ftsZ"
                     /locus_tag="PKY13_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT80850.1"
                     /translation="MAHNPQNYLAVIKVVGIGGGGCNAVNRMIDAGLKGVEFIAINTD
                     AQALLMSDADVKLDIGREVTRGLGAGSDPEVGRQAAEDHKAEIEEVLRGADMVFITAG
                     EGGGTGTGGAPVVAEVAKAIGALTIAVVTRPFLFEGRRRSVQAEQGIGKLKEKVDTQI
                     VIPNDRLLSVANDKTSVLNAFKMADEVLLQGVQGITDLITTPGLINTDFADVKMIMHD
                     AGTALLGVGYASGEGRAMSAARQAISSPLLESAIDGARGILLNICGGSDLTLFEVNEA
                     ATVIHEVAHAEANIIFGTTIDDEMGDEVRVTVIAAGFERWEDPKAAAAAASSRRSSSG
                     SSGGREERRPRSDDDIPLADVFADDDDDDSGDSDADDFDVPSFLK"
     gene            complement(5010..>5323)
                     /locus_tag="PKY13_12730"
     CDS             complement(5010..>5323)
                     /locus_tag="PKY13_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80851.1"
                     /translation="PSDLVGEQRSDAERKLLATKTMLGPQIDLRGVCQLDVRVPTAPT
                     MRRNPDCDPPPPAPVVDPATGATIDPATGQPVAPPETDVAPEAPATTTPPDPATTVPS
                     A"
BASE COUNT          775 a         1802 c         1883 g          763 t
ORIGIN      
        1 gaactcggtg aaggcgtgtg aggccgtcgt cgcggtggcc tctcgctcgt agacctcacc
       61 ttcgggagcg aacgccgccg acgagtccgg gaacgaggcg acctcgcccc agctgcccgg
      121 gccccacgga tccccgacgg gcgcctcgtc ggcacggtcg tcgaggcgat cgtcggacca
      181 cggaaggtcg gcgccgccgg ccagttccgt ctcctcggag tacggcacgg ccaggtcgtt
      241 ggggtcgagg agtgtggcct cgccgaagtg agagccgctc gagccgttgc ggaacgccgg
      301 gtcgtcgtcg gagaacgcga catcggcgaa accagcgtcg gagaacgcgg gctcggagaa
      361 tgcggcgtcg gcaaaggccg gctcggaccc gaccggctcg ccgaacgggg agtcggtgaa
      421 ggccggttcg ccgatcggct cggcgtagga cggctcggtg tcgaaggacc gcgcgtcggg
      481 ggcgacgtcg gcaacggggg cgaacacggc cggctcgtag ggggcggcgt cgaacggggc
      541 gtcgtcagag gacgccacgt cggcaggggc gacgtcgggt tcgggcaccg gctcggcgac
      601 cgggcggacg atgccggcca ggtcgatgcc gggcagcggc gagcgcagcg ccgtctcggg
      661 atcgtcgagc accgcaccga tcgcgccgtg gacgctggtg agcgccgagc ggtactcctc
      721 gagacgaccc tcgatcgcga ccagctggga ggccataccg accttgcggt tctcgagttc
      781 gccgatctcg gcgaggatct ggtcgcggcg ggccccgtac tcacggttgg cctcggccgt
      841 tgccttctgg gtcatctggt cggcgttgac gcgcgcctcg ccgacgatgc ggtcggcctc
      901 ggtgcgggcc tcgcgagcct gctgctcggc ggcggtgcga gcatcggcca cgagcttctg
      961 ggcttcctgc cgggcgtcgg cgatggcggc gtcggcggtg cgcttcgcca tcatcagcgt
     1021 gcgcgtcgac tgcatcgcct cttcttcgtc cgacatcgct gccgctgccg ccgccggggc
     1081 gcgaccggcc tcgggtgcat tgcgcagctc ggcctcggcg cgctcggcgc gggcgatggc
     1141 gtcacgtgac gtcttggtca gctcggccag ggtggccccg acccgctcga ggaagtcgtc
     1201 gacctgatcg gggtcatagc ctcgaaactt ctccgcgaag tcgatttgct cgatgagctg
     1261 aggtgtcacg tccatgacgt gcatgctagc gacgactttt tcaaacgcgg cggaaccagc
     1321 taccaaaact tctgcccgag ggcaagaaga tcttcgtccg gtccgagggg ggccgggtgg
     1381 acccccaccg cgcccgcgac gaactgccgg ggactactcc tcgacgaggc cgcgttcgcg
     1441 gaggcgacgg cggtcgtcgg gcgagatctc gacgtcgatc ggcgacagga ggtacacgtg
     1501 ggatgccacg cgctcgacgc tgccgccgag gccgtaggcc acaccgctcg agaagtcgag
     1561 caggcgccgg gccagatcgc gatcgaggtc ctgcaggttg acgatgacgg gctggcgctt
     1621 gcggaaccag tcgccgactt cctgggcgtc gttgaacgtc ttcggcgtga cgacgtgggg
     1681 ccgggccgac gccgaggagg cggcgggcac ggggcggacc acgctgctgc gggggcggac
     1741 cggctcggga gcggtcacca caccaccacg ggggcgacca ccggcggacg gcaacacccg
     1801 aacggacgcc gactcgaccg gctcctccca gtcgggcatg ggctcgaccc gcgacggggc
     1861 cggcacggcg cgcacgaccg ggctcgggtt gacggtgccg gtactgaccg cacggtgagg
     1921 acccgtgagg tcgacgacgg gatcttcttc gtagtcgtcg taatcgtcgt actcatcatc
     1981 gggacccagt cccaaccacg acataccttt gcgcaacatc ccagccatca actcctcctc
     2041 atgacctcga ccagtgtcgc gcacgaggaa ctgcccgacg gggaccagag tgccatgaat
     2101 catcgccgcg tgacagttcg cggtggttca aggtctcgaa ccgaagatgt cggtacccac
     2161 gcggaccatg gtcgaaccct cggcgatcgc gatctcgagg tcgccggaca tgccgatcga
     2221 gcactccgga agggcctcgg cgtcgcacag tgaccgcaac cgaccgaacg cggcggcgac
     2281 cgtcgcgtcg tcggcgaggg gcgcgacacc catcgcgccc acgaccgaca gcccggcggc
     2341 gcgggccgac tcgatgagcc ccggagcgtc ctgccacgac actcccccgc gaccttcgac
     2401 gtcggcgagg ttcacctgca ccatcaccgt cgcgcccgac actcgcttgg cgagctcggc
     2461 gacgagggac ggccggtcga ccgattggta cacgtcggtc cacggcgcga cgacgcgcac
     2521 cttgttcgtc tgcagctgtc cgatgaagtg ccagcggacg gccgcgccga cctcgggcgc
     2581 cttcgctgcg aactccacgg cgtagctctc gccgaactcg acgaaccccg cagcggcagc
     2641 ggcgacgatc gtctcggccg gatgcgtctt ggacacggcg atgagccgga cccccgactc
     2701 ggcgaacgac ggcgcgccgg aggccacgcg gatccgttcc tcgatcgccg cccgcctcgc
     2761 ggccagccga tcgacgagat ccgggtcggg aacgatgctc acgagccggg ccccgtcacg
     2821 gctcgatcca gatgaccgag gcctggcgac cggtgtcggc ccgtgcccgg tgggagaaga
     2881 agcgcggcgt tccgtcggcg ccgatcgtgg tcgcggtgca cgccgcggcg gccacgtcga
     2941 cgtcgtcgat cccgaccgaa cggagcgcgc tgcgaaccgc cgcggtcatg tcgagggccg
     3001 cgtcgccgcc ctcggtcgct ccgaccacct cgggtccgaa acggagtgcc atcgtcgtca
     3061 ggtcccgctc ggagaactcg taggccgctg gcgagataca ggggccgagg cgtgcggaga
     3121 tgtcacgggc acccaggtcg atcagcgcgc cgaccgtcgc ctcgaggacg ccctcgtaga
     3181 ggcccttcca cccggcgtgc gccgcaccga tcannnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnngcctcga ggacgccctc gtagaggccc ttccacccgg cgtgcgccgc
     3361 accgatcacc ggccctgccg acgggcccgg cccgacggcg gtgaacagga ccggtgcgca
     3421 gtcggccgtc tgcacggcca gcacggcccc gggggcgctg gtcaccgccg cgtcggcccg
     3481 ggcgccggcc cactggcggg gccgcgacac cacgacgacg cccgcaccgt gcacctgctc
     3541 gagccacgtc cacgggtgcg gcgccagcgc cgagcgcgcc gcagcgagcg cacgcggctc
     3601 ggcggtgatc gcgaaatcgc cgtcggcccg ttcgctgaag cggtgctgga ccgccacgac
     3661 cggcccctac ttcaggaagc tggggacgtc gaagtcgtcg gcgtcggaat cgccggagtc
     3721 gtcatcgtcg tcgtcggcga acacgtcggc gagcgggatg tcgtcgtcgg aacgggggcg
     3781 acgctcctcg cgcccacccg acgacccact cgagctgcgc cgcgacgagg ccgccgcggc
     3841 tgcggccttg ggatcttccc accgctcgaa cccggcggcg atgaccgtga cacggacctc
     3901 gtcgcccatc tcgtcgtcga tggtcgtgcc gaagatgatg ttggcctcgg catgcgccac
     3961 ctcgtggatg accgtcgccg cctcgttgac ctcgaacagc gtgaggtcgg agccgccgca
     4021 gatgttgagg aggatgccgc gggcgccgtc gatcgccgac tcgagcagcg gcgagctgat
     4081 cgcctggcgg gcggccgaca tggcccgacc ctcgcccgag gcgtaaccga cacccaggag
     4141 tgcggtgccg gcgtcgtgca tgatcatctt gacgtcggcg aagtcggtgt tgatcaggcc
     4201 cggcgtcgtg atgaggtcgg tgatgccctg cacaccctgg agcaggacct cgtcggccat
     4261 cttgaacgcg ttgagcaccg acgtcttgtc gttggcgacc gacagcagcc ggtcgttggg
     4321 gatgacgatc tgggtgtcga ccttctcctt gagcttgccg atgccctgct cggcctgcac
     4381 cgagcgccga cggccctcga agaggaaggg acgggtgacg acggcgatgg tgagcgcgcc
     4441 gatcgccttg gcgacctcgg ccacgaccgg tgcgccaccg gtaccggtgc cgccgccttc
     4501 gccggcggtg atgaacacca tgtcggcgcc gcggagcacc tcttcgatct cggctttgtg
     4561 gtcctccgcc gcctggcgac cgacctcggg atcggagccc gcaccgaggc cacgggtgac
     4621 ctcacgaccg atgtcgagct tcacatccgc gtcgctcatc agcaacgcct gggcgtcggt
     4681 gttgatggcg atgaactcca cacccttcaa cccggcgtcg atcattcggt tcacggcgtt
     4741 gcagccgccc ccaccgatgc cgaccacctt gatgactgcg aggtagttct gcggattgtg
     4801 agccattggg aggggacctc cgatgcgaac cggcgacggc tgcttcgtgc aaattgtggg
     4861 gagacggaat gatggtgcga accgaagatc gcaggcaatc gggcagtgcg atccgaccga
     4921 gaatcgggac ggaccgaccc actctaggtg aactctccac ggggggtcga gtgtcacact
     4981 cccgtggacc ttgaccgtcg aggtccggtt caggccgacg gcaccgtggt ggcggggtcg
     5041 ggtggggtcg tggtcgccgg cgcctccggc gcaacgtcgg tctcgggcgg ggcgacgggc
     5101 tgacccgtgg cgggatcgat cgtcgcgccc gtggccggat cgacgacggg ggccggtggc
     5161 ggcggatcgc agtcggggtt gcgccgcatc gtcggcgccg tgggtacccg gacgtcgagc
     5221 tggcacacgc cgcgcaggtc gatctggggg ccgagcatcg tcttggtggc gagcagcttg
     5281 cgttccgcgt cgctacgctg ctcgcccacg aggtcgctgg gga
//
LOCUS       DAOSOA010000231         5320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15564_length_5320_cov_6.041406, whole genome shotgun sequence.
ACCESSION   DAOSOA010000231 DAOSOA010000000
VERSION     DAOSOA010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15564_length_5320_cov_6.041406"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKY13_12735"
     CDS             complement(<1..920)
                     /locus_tag="PKY13_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80852.1"
                     /translation="MFSRLNVRTKLLVMLLPAALGLCGLAGLGVKTRLDDRNAAQTTR
                     EASEVAATASAYVHRLQVERVAAATVAAAPSTELEATLVTERIATDEAGSRLDTVLDR
                     SGETLGGDAAVAKDLGALRRGRGQLDATRTSGTTAAATTEAYSNLIDGHLAAVGRLEL
                     VADGAASTTASRRWLAAGIEAESRSAATAAGLFSADATPEGRAADAVAARELVERARM
                     FYDTYEDYAGTEELGRFDAARSSVGSARTDDAFAELATPDAASTFGADADTWPASVRD
                     RLDAVVGVERAGYNADIARFAAAAEAADSSV"
     gene            1079..1810
                     /locus_tag="PKY13_12740"
     CDS             1079..1810
                     /locus_tag="PKY13_12740"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOT80853.1"
                     /translation="MNAATTLQGTIGDIAVDDVLRLLESRRQTGVLLVNPTNPLRLVL
                     ADGAIVLGVSSSTMAIGRFLLACGMVTAEQLEELLGLVRSRLGERQTRSLDEIQVIEM
                     LVDTVPAADLRVAVRSQAVAVMFETMALPDASWTFTEAAPHPLAERFAFGVGELLESA
                     DEQIRAWPELRQRVGDDATHLRRVPRLGAHQTPVILDAVDWAALSEIDERSTVGQIGH
                     RLGLGRPDAYAVLAGLLDRGVVEPG"
     gene            complement(1830..3299)
                     /locus_tag="PKY13_12745"
     CDS             complement(1830..3299)
                     /locus_tag="PKY13_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOT80854.1"
                     /translation="MGKPTGFIEHGRELPKRRPVPVRLRDWKEVYEEFPDDHLQVQAS
                     RCMDCGIPFCNNGCPLGNIIPDWNDLVYRDRWQAAIERLHATNNFPEFTGRLCPAPCE
                     GACVLGINQDPVTIKQVEVSIIDRAWEEGWVTPLRPSQETGKSVAVIGSGPAGLAAAQ
                     QLTRAGHHVVVYERADRIGGLLRYGIPEFKMEKRHLDRRIAQMRAEGTVFLCNMDVGT
                     DITVEQLKAKYDAIVLAVGATQSRDLPIPGREAIGVHLAMEYLPWANRAQEGDLAPED
                     TPINANGKHVVIIGGGDTGADCLGTAHRQGAASVRQFEIMPRPVDGRPTANPWPTYPM
                     SYKVTSAHEEGGERVYAVNTERFVVGDDGQLVGLEYHEVEQKFTDGRMSFERVEGSDT
                     TLDVDLVFLAMGFTGPERGPLLDQLGVDLDDRGNVARSSEWATNVEGVFVAGDAGRGQ
                     SLIVWAIAEGRACAAAVDTFLEGHSDLPAPIVPTDRPLV"
     gene            complement(3423..>5320)
                     /locus_tag="PKY13_12750"
     CDS             complement(3423..>5320)
                     /locus_tag="PKY13_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOT80855.1"
                     /translation="YGSISAEAHETLAIAMNRLGGKSNTGEGGEDPERFVPLDNGDSK
                     RSSIKQVASGRFGVTSEYLTNADDIQIKMAQGAKPGEGGQLPGHKVYPWVAKTRHSTP
                     GVGLISPPPHHDIYSIEDLAQLIHDLKNSNPAARVHVKLVAEVGVGTVAAGVAKAKSD
                     VVLISGHDGGTGASPLTSLKHAGAPWELGLAETQQTLLLNDLRDRIVVQADGQLKTGR
                     DVIIAALLGAEEFGFATAPLVVSGCIMMRVCHLDTCPVGVATQNPLLRERFSGKPEFV
                     ENFMLFIAEEVRELLAELGFRTLEEAVGHVERIDAEAAIDHWKASGLDLSPILHLPEL
                     AEGAARHNTTTQDHGLDRALDRWLIEGARPALDDGQRVRLRHTIRNVNRTVGTLLGHE
                     LTKVYGGDGLPDDTIEVDFVGSAGNSFGAFVPRGITLRLEGDANDYVGKGLSGGRLVV
                     RPPADSPFVSEDNVIAGNVIGYGATAGEIYLRGRVGERFCVRNSGATAVVEGVGDHGC
                     EYMTGGRVVVLGETGRNFGAGMSGGIAYVYDPDERFVALLNGEMVMIEELDQGDRDFL
                     EITVGRHVAETGSEVGRKILEDVPASLAAFRKVMPRDYRKVLDAIALAESEGRDVDEA
                     IMESART"
BASE COUNT          745 a         1851 c         1894 g          830 t
ORIGIN      
        1 accgaggagt cggcggcctc ggcagcagcg gcgaaccggg cgatgtcggc gttgtagccg
       61 gcacgctcga cgccgacaac ggcatcgagt cggtcgcgga ccgacgccgg ccacgtgtcc
      121 gcatcggcgc cgaaggtcga cgcggcgtcg ggggtggcga gctcggcgaa tgcgtcgtcg
      181 gtccgagcgg agccgaccga cgaacgagcg gcgtcgaagc gtccgagctc ctcggtgccg
      241 gcgtagtcct cgtaggtgtc gtagaacatg cgggcccgct cgacgagctc ccgggccgcg
      301 acggcgtcgg cggcacgccc ctcgggcgtg gcgtcggcgg agaagagccc tgctgcggtc
      361 gctgcggatc gggactcggc ctcgattccg gcggcgagcc accgccggct ggccgtggtc
      421 gaggcggccc cgtcggcgac gagttcgagc cggccgaccg ccgccagatg gccgtcgatc
      481 aggttcgagt acgcctcggt ggtggccgca gcagtggtgc ccgacgtccg ggtcgcgtcg
      541 agctgaccgc gcccgcgacg cagcgcgccg aggtccttcg cgacggcggc atcgccgccg
      601 agcgtttcgc cgctccgatc gagcacggtg tcgagccggg aaccggcctc gtcggtggcg
      661 atccgctccg tgaccagggt cgcctcgagc tcggtcgacg gggcggccgc gacggtcgcg
      721 gcggcgaccc gttcgacctg gagccggtgg acgtaggccg acgccgtcgc agcgacctcg
      781 gatgcctccc gggtcgtctg tgcggcattt cggtcgtcga gccgagtctt cacgccgagg
      841 ccggcgagcc cgcacagtcc caacgccgcc ggcaggagca tgaccagcag cttcgtacgg
      901 acgttgagtc tggagaacat gtgatcctct gatgatcgct gccggtgccc ctgtggctcg
      961 gcccacggcg gttccaccgc ctcggctctg tatcggccac tccccgcggg cccttgaaaa
     1021 cagcgatccc ctcggcgcag gggtgattcc gccccccgcc tcgttagcct ttgcggaggt
     1081 gaacgcagcc accaccttgc agggcaccat cggcgacatc gccgtcgacg acgtgctgcg
     1141 cctgctcgag agccggcgcc agaccggtgt gctgctcgtc aacccgacca accccctgcg
     1201 tctcgtcctc gccgacggcg cgatcgtgct gggcgtgtcg tcctcgacga tggcgatcgg
     1261 ccggttcctg ctcgcgtgcg ggatggtgac ggccgaacag ctcgaggagc tgctcgggct
     1321 cgtccggagc cgtctcggcg agcgtcagac ccgcagcctc gacgagatcc aggtcatcga
     1381 gatgctcgtc gacaccgtgc cggcggcgga cctcagggtc gccgtccggt cacaggccgt
     1441 tgccgtcatg ttcgagacga tggcgctccc cgacgcctcg tggacgttca ccgaggcagc
     1501 gccccacccc ctggccgagc ggttcgcgtt cggcgtcggc gagctcctgg agtcggccga
     1561 cgaacagatc cgggcgtggc ccgagttgcg ccagcgggtc ggggacgacg ccacccacct
     1621 tcgccgcgtg ccccgtctgg gcgcgcacca gaccccggtg atcctcgacg ccgtcgactg
     1681 ggcggcgctc agcgagatcg acgagcggtc gacggtcggc cagatcggcc accgcctcgg
     1741 cctcgggcgt cccgacgcct acgcagtgct cgccggcctg ttggaccgcg gcgtcgtcga
     1801 gcccggctga ccaacacgcc cctcggggtt cagaccaggg ggcgatccgt cggcacgatc
     1861 ggtgcgggca ggtcgctgtg gccctcgagg aacgtatcga cggctgccgc acacgcgcgg
     1921 ccttcggcga tcgcccacac gatgaggctc tggccgcggc cggcatcgcc ggcgacgaac
     1981 acaccctcga cgttggtggc ccactcgctc gaccgggcga cgttgccgcg gtcgtcgagg
     2041 tcgacgccga gctggtcgag cagcggaccg cgctcggggc cggtgaagcc catggccagg
     2101 aacacgagat cgacgtcgag cgtcgtgtcg gacccttcga cccgctcgaa gctcatccgg
     2161 ccgtcggtga acttctgctc gacctcgtgg tactcgaggc cgacgagctg gccgtcgtcg
     2221 ccgacgacga accgctcggt gttgacggcg tagacccgct ccccgccctc ctcgtgggcg
     2281 gacgtcacct tgtagctcat cgggtaggtc ggccacgggt tggccgtcgg acgaccgtcc
     2341 accgggcgcg gcatgatctc gaactggcga accgacgccg caccctgccg gtgcgcggtg
     2401 ccgaggcagt cggcgccggt gtcgccgccg ccgatgatga cgacgtgctt gccgttggcg
     2461 ttgatgggcg tgtcctcggg ggcgaggtcg ccctcctgag cgcgattcgc ccacggcagg
     2521 tactccatcg cgagatggac gccgatggcc tcgcggccgg ggatcggcag atcgcggctc
     2581 tgggtcgcgc cgacggccag cacgatcgcg tcgtacttgg ccttcagctg ctcgacggtg
     2641 atgtcggtgc cgacgtccat gttgcagagg aacacggtgc cctcggcgcg catctgggcg
     2701 atgcggcggt cgaggtgccg cttctccatc ttgaactcgg ggatgccgta acggagcagc
     2761 cccccgatgc ggtcggcgcg ttcgtagacg acgacatggt gcccggcgcg ggtgagctgc
     2821 tgagcggcag cgaggcccgc gggaccggaa ccgatgacgg cgaccgactt gcccgtctcc
     2881 tggctgggac ggagcggggt cacccagccc tcctcccagg cacggtcgat gatcgagacc
     2941 tcgacctgct tgatggtgac cgggtcctgg ttgatgccga ggacacacgc gccttcgcac
     3001 ggcgccgggc agagccgacc ggtgaactcg gggaagttgt tcgtcgcgtg gagccgctcg
     3061 atcgccgcct gccaacggtc ccggtacacg aggtcgttcc agtcggggat gatgttgccg
     3121 agcgggcagc cgttgttgca gaagggaatc ccgcagtcca tgcaccggct cgcctggacc
     3181 tggaggtggt catcggggaa ctcctcgtag acctccttcc agtcgcgcag gcgaacggga
     3241 acggggcggc gcttgggcag ctcgcggccg tgctcgatga atccggtggg cttacccatg
     3301 aatcgtcaat ccttcgatga gagcactgca tttgtcctgc agatccgccc ctccaggggc
     3361 ggaatcgcag gacagaacgg gtgaggtcag ggccgtcggg tgcgtcatgt gagtcatgtg
     3421 cgtcatgtgc gggccgattc catgatcgcc tcgtcgacgt cgcggccctc ggactcggcg
     3481 agcgcgatgg catcgagcac cttgcggtaa tcgcggggca tgaccttgcg gaacgccgcc
     3541 aggctggcgg gaacgtcctc gaggatcttg cggccgacct ccgagcccgt ctcggccacg
     3601 tgtcggccga ccgtgatctc gaggaagtcg cggtcgccct ggtcgagttc ctcgatcatg
     3661 accatctcgc cgttgaggag ggcgacgaag cgctcgtcgg ggtcgtacac gtaggcgata
     3721 ccgcccgaca tgccggcgcc gaagttgcgc ccggtctcac cgagcaccac cacccgaccg
     3781 ccggtcatgt actcgcagcc atggtcaccc acaccctcga cgaccgccgt ggcaccggag
     3841 ttgcggacgc agaagcgctc gccgacccgg ccccgcaggt agatctcgcc ggccgtcgcg
     3901 ccgtagccga tcacgttgcc ggcgatgacg ttgtcctccg acacgaacgg gctgtcggca
     3961 ggcgggcgga cgaccaaccg gccgcccgac aggcccttgc cgacgtagtc gttggcgtcg
     4021 ccctcgaggc gcaacgtgat accgcggggc acgaacgcac cgaagctgtt gcccgccgag
     4081 ccgacgaagt cgacctcgat cgtgtcgtcg ggaagcccgt cgccgccgta caccttggtc
     4141 agctcgtggc cgagcagcgt gcccaccgtg cggttgacgt tgcggatcgt gtggcgcagg
     4201 cgcacccgct gaccgtcgtc gagcgccggg cgggcgccct cgatcagcca gcggtcgagg
     4261 gcacggtcga gcccgtggtc ctgggtcgtg gtgttgtgcc gcgccgcacc ctccgccagc
     4321 tcgggcaggt ggaggatcgg cgacaggtcg agacccgacg ccttccagtg gtcgatcgcg
     4381 gcttcggcat cgatgcgctc gacgtggccc acggcctcct cgagcgtccg gaaacccagc
     4441 tcggcgagga gctcgcggac ctcctcggcg atgaacagca tgaagttctc gacgaactcg
     4501 ggcttgccgc tgaagcgctc ccgcaggagc gggttctggg tggcgacgcc cacggggcag
     4561 gtgtcgaggt ggcagacccg catcatgatg cagcccgaga caacgagggg cgccgtggcg
     4621 aacccgaact cctcggcgcc gagcagcgcc gcgatgatca cgtcacggcc ggtcttcagc
     4681 tggccgtcgg cctgcacgac gatgcggtcg cggaggtcgt tgagcagcag cgtctgctgg
     4741 gtctcggcca acccgagctc ccacggggcg ccggcgtgct tgagcgacgt cagcggcgag
     4801 gcgccggtgc caccgtcgtg cccgctgatg agcaccacgt cggacttggc cttcgcgacg
     4861 ccggcggcga ccgtgccgac cccgacctcg gccacgagct tcacgtgcac acgcgccgcc
     4921 gggttcgagt tcttgaggtc gtggatcagc tgggccagat cctcgatcga gtagatgtcg
     4981 tggtgcggcg gcgggctgat gaggcccacg cccggcgtcg agtgccgcgt cttcgcgacc
     5041 cacgggtaca ccttgtggcc cggcagctgg ccgccctcgc cgggcttggc gccctgcgcc
     5101 atcttgatct ggatgtcgtc ggcgttggtg aggtactccg acgtcacacc gaaccggccc
     5161 gacgccacct gcttgatcga gctccgcttg gagtcgccgt tgtcgagcgg cacgaagcgc
     5221 tccggatcct cgccgccctc accggtgttg gacttgccgc ccagacggtt catggcgatg
     5281 gcgagggtct cgtgcgcctc cgcggagatc gagccgtagc
//
LOCUS       DAOSOA010000232         5318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15573_length_5318_cov_8.047501, whole genome shotgun sequence.
ACCESSION   DAOSOA010000232 DAOSOA010000000
VERSION     DAOSOA010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15573_length_5318_cov_8.047501"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PKY13_12755"
     CDS             complement(<1..1235)
                     /locus_tag="PKY13_12755"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOT80856.1"
                     /translation="MSELDLLVLGSGVAGLSAAVRAAELKGLSVGVMTKAELELATTR
                     WAQGGVAATLSGDREETDLHLADTLAAGVGLCDIEAVRVLVDEGPGRVHDLIALGALF
                     DRDAEGELELAREGGHSVARIVHAGGAATGAEIERALVAAVRERVAEIYEETFALDLM
                     IQDGRCRGVRAVGADGEVFEVRARHVLLATGGAGQLFAVTTNPPVATGDGVAMALRAG
                     VAIADMEFFQFHPTALDHPSMPRPLLSEALRGHGALLRDANGERFVDELLPRDVVSRA
                     ITARMLDQDIDHVWLDATQLDHFHIRFPTIAADLEAAGLDPSVDYLPVAPAAHHQCGG
                     IVTDINGATTLPGLWAAGETSCSGVHGANRLASNSLLEGMVFGARVVESIAEGIDGPE
                     DTGAMRSARGVPSTDILIGG"
     gene            complement(1228..2124)
                     /gene="panC"
                     /locus_tag="PKY13_12760"
     CDS             complement(1228..2124)
                     /gene="panC"
                     /locus_tag="PKY13_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOT80857.1"
                     /translation="MQTVTTIAELRRHLDAARAVGRTVGLVPTMGYLHAGHESLMVRA
                     SAECDDVVATIFVNPLQFAPDEDLDAYPRDLEGDTALATRAGVDWLFVPSVEEMYPRP
                     VATTVSVAGVSAAMEGASRPTHFDGVATVVTKLFNIVGPCHAYFGEKDFQQLAVIRRM
                     VSDLSVPVVVVGCPTLREDDGLAKSSRNVYLSADERVAAPVLHRALRLGAEMIAAGET
                     DADVVRAAMAAEIATQSYGALDYVEVADADTLELQQVCGPNSRLVGAVRFGRARLIDN
                     IGVGNVAQPVPATGTARGAHDV"
     gene            complement(2126..2854)
                     /locus_tag="PKY13_12765"
     CDS             complement(2126..2854)
                     /locus_tag="PKY13_12765"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOT80858.1"
                     /translation="MTRRVRIIGAGRAGGSFASALASVGWEVDGPIGRDAGAIAAAAS
                     DVDLVLICVGDGAVPDVAASIEPEPTCVVAHCAGSLGLDVLAPHERRAAVHPLVSLPN
                     PTTGTERLRDDAWFATAGDGIADEVVDALGGRSFVIADEHRVEYHAAACIAANHLVAL
                     LGQVERIGAAAGAPFEAYLSLVRGAVDNVAELGPAAALTGPAARGDELTIARHLAAID
                     PSERDAYRAMADQARRLVTTNGSN"
     gene            complement(2851..3303)
                     /gene="folK"
                     /locus_tag="PKY13_12770"
     CDS             complement(2851..3303)
                     /gene="folK"
                     /locus_tag="PKY13_12770"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOT80859.1"
                     /translation="MRTFISVGSNLGDRRAFLRDAVDSLPDVVAVSSVFETDPMGGPG
                     NQGAFLNIVVELDTELTPSALLSVCHRIESAAHRVRDERWGPRTLDLDIIWMDGVTMD
                     EPRLTIPHPRWKERKFVLAPLRELAPDLVTAQEVEIADGRVWTVENLS"
     gene            complement(3303..3665)
                     /gene="folB"
                     /locus_tag="PKY13_12775"
     CDS             complement(3303..3665)
                     /gene="folB"
                     /locus_tag="PKY13_12775"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOT80860.1"
                     /translation="MADVIQLRGLRALGFCGALPEEQERRQPFEVDLDVETDLSAAGA
                     SDALDDTVDYGALCAQVEAVIVDERFALMERMAARLAEVVLGDSRITAVTVTVRKLRP
                     PVPQQLETSGVTIRRTND"
     gene            complement(3669..4193)
                     /locus_tag="PKY13_12780"
     CDS             complement(3669..4193)
                     /locus_tag="PKY13_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80861.1"
                     /translation="MAAKGKWAQGIKPRHFSWVMQDKLAICERPGGYGTNHRKVRRQE
                     EIIWIREQGFAPVISLIPANHNLHNYDELSVTWRHRPLPATEDYPRYLGDIYPELQGL
                     LDSGAKVLYHHEELNDRLVGIMAGYLVWAGIVVEQPRAIFLLEHITSRQLGPAGRDIV
                     AVAAKIRANAAAAE"
     gene            complement(4204..5040)
                     /gene="folP"
                     /locus_tag="PKY13_12785"
     CDS             complement(4204..5040)
                     /gene="folP"
                     /locus_tag="PKY13_12785"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT80862.1"
                     /translation="MIPQIMGILNVTPDSFSDGGRYVDLDAAVTQARRLVDEGASFVD
                     VGGESTRPGAAPVSIDEELARVVPVIEAITALDGFGARISVDTRRAEVARAAVAAGAT
                     VINDVSASLWQVAADLGVGWVAMHMQGEPCSMQDDPTYVDARTEVYDFLTERAATAHS
                     AGVAEVWVDPGFGFGKSLDHNLELVAGIGDLVDRGTPVLLGVSRKKSLGVLTARADGL
                     GADVVSPPSDRLEMGVALATWAMMAGAQIIRAHDVRPHVEAALAVAAARSNTPTPAVA
                     GN"
BASE COUNT          808 a         1845 c         1905 g          760 t
ORIGIN      
        1 ccgccgatga ggatgtcggt cgacgggacg cctcgggccg aacgcatcgc gccggtgtcc
       61 tcgggaccgt cgatgccctc cgcgatcgac tcgacgaccc gggccccgaa caccatgccc
      121 tcgagcaacg agttcgacgc gagccggttc gccccgtgca cccccgagca gctcgtctcg
      181 ccggccgccc acagacccgg gagggtggtc gccccgttga tgtcggtgac gatgccgccg
      241 cactggtgat gggcggccgg ggccaccggc aggtagtcga ccgacgggtc gagaccggcc
      301 gcctcgagat cagcggcgat ggtggggaaa cggatgtgga agtggtcgag ctgcgtggca
      361 tcgagccaga cgtggtcgat gtcctgatcg agcatgcgcg ccgtgatggc gcgggacacg
      421 acgtcgcggg gcagcagctc gtcgacgaag cgctcgccgt tggcatcgcg cagcagggcg
      481 ccgtgtccgc ggagggcctc ggacaagagc gggcgcggca tcgaaggatg gtcgagcgcc
      541 gtcggatgga actggaagaa ctccatgtcg gcgatggcaa cgccggcgcg aagcgccatc
      601 gcgaccccgt cgccggtggc gaccggcggg ttggtggtga cggcgaacaa ctggccggca
      661 ccaccggtgg cgaggagcac gtggcgcgcc cgaacctcga acacctctcc gtcggcaccc
      721 accgcccgga cgccgcgaca gcggccgtcc tggatcatca ggtcgagcgc gaaggtctcc
      781 tcgtagatct cggcgacccg ttcgcggacg gctgcgacga gtgcccgctc gatctcggcg
      841 ccggttgcgg cgccaccggc gtgcacgatg cgggccaccg aatgcccgcc ctcgcgggcg
      901 agctccaact caccctcggc atcacgatcg aagagggctc ccaacgcgat gaggtcgtgg
      961 acccggcccg gcccttcgtc gacgagcacg cgcaccgcct cgatgtcgca caacccgaca
     1021 ccggccgcga gcgtgtcggc caggtggagg tcggtctcct cacggtcgcc cgacagggtg
     1081 gcggccaccc cgccctgggc ccagcgggtg gtggccagtt cgagctcggc cttggtcatg
     1141 acaccgacgc tgagaccctt cagttccgcc gctcgcaccg cggccgacaa cccggccacg
     1201 ccgctgccga gcaccagcag atcgagttca gacatcgtgg gctcctcgcg ccgtaccggt
     1261 ggccggaaca ggctgcgcga cgttacccac tccgatgttg tcgatgagtc gcgctctccc
     1321 gaagcggacg gcacccacga gtcggctgtt cgggccgcag acctgctgga gttcgagggt
     1381 atcggcgtcc gcgacctcga cgtagtcgag ggccccgtac gactgggtcg cgatctccgc
     1441 cgccatggcg gcgcgcacca cgtcggcgtc ggtctcgccg gcggcgatca tctcggcacc
     1501 gagccggagc gcacggtgga ggaccggcgc cgcgacacgt tcgtcggcag agaggtagac
     1561 gttgcggctc gacttcgcga gcccgtcgtc ctcgcggagc gtgggacacc cgacgacgac
     1621 gacgggcacc gagaggtccg acaccatgcg acggatgacg gccagttgtt ggaagtcctt
     1681 ctccccgaag tacgcgtgac acgggccgac gatgttgaac agcttggtga ccacggtcgc
     1741 gacgccgtcg aagtgcgtcg gcctcgaggc accctccatg gccgctgaca ccccggcgac
     1801 cgacacggtc gtcgcgaccg gacgggggta catctcctcg accgacggca cgaacagcca
     1861 gtcgacaccg gctcgggtcg ccagcgcggt gtcgccctcg aggtcgcggg ggtaggcgtc
     1921 gagatcctcg tcgggggcga actgcagcgg gttgacgaag atcgtcgcaa caacgtcgtc
     1981 gcactcggcg ctcgcccgga ccatcagcga ctcgtgaccg gcgtgcaggt accccatggt
     2041 cgggacgagt ccgacggtgc gaccgacagc gcgggcggcg tcgaggtggc ggcgcagctc
     2101 ggcaatggtc gtgacggtct gcatgtcagt tgctcccgtt ggtggtgacg agacggcggg
     2161 cctggtcggc catggcccgg taggcgtcgc gctcggacgg gtcgatcgcc gcgaggtggc
     2221 gggcgatcgt cagttcgtcg ccgcgcgccg ccggcccggt gagcgccgcg gccgggccga
     2281 gctcggcgac gttatcgacg gccccgcgca cgagcgacag gtaggcctcg aacggcgcgc
     2341 cggcggccgc cccgatccgt tcgacctgac cgagcagagc gaccaggtgg ttggcggcga
     2401 tgcaggcggc ggcgtggtac tcgacgcggt gctcgtcggc gatgacgaag gaacgcccgc
     2461 cgagcgcgtc gacgacttcg tccgcgatcc catcaccggc cgtggcgaac caggcgtcgt
     2521 cgcgaagtcg ttcggtgccg gtcgtggggt tcggcagcga cacgagcgga tgcaccgccg
     2581 cccgccgctc gtggggagcg agaacgtcga gcccgagcga cccggcacaa tgggccacaa
     2641 cgcatgtcgg ctcgggttcg atcgaagcgg ccacgtcggg caccgcgccg tccccgacgc
     2701 agatcaacac gagatcgacg tcgctcgccg cggccgcgat cgctccggca tcccggccga
     2761 tcgggccgtc cacctcccag ccgacggagg ccagggccga ggcgaacgag ccgccggcgc
     2821 gcccggcgcc gatgatccgg acccgtcgcg tcacgacagg ttctcgaccg tccacacccg
     2881 cccgtcggcg atctcgacct cctgggccgt caccaggtcg ggcgcgagct cgcgcagcgg
     2941 cgccagcacg aacttccgtt ccttccagcg cgggtgcggg atggtgagcc gaggctcgtc
     3001 catcgtgacg ccgtccatcc agatgatgtc gagatcgagg gtccgcgggc cccagcgctc
     3061 gtcacgcacc cgatgggccg ccgactcgat ccggtgacac acgctcagca acgcgctcgg
     3121 cgtcagctcg gtgtcgagtt cgacgacgat gttcaggaac gcaccctgat tgccggggcc
     3181 acccatcggg tcggtctcga acacgctcga caccgcgacc acgtcgggca gcgaatcgac
     3241 ggcgtcgcgg aggaacgctc gacggtcgcc gaggttcgac ccgacgctga tgaacgtccg
     3301 catcagtcgt tggtccgacg gatggtcacg cccgaggtct cgagctgttg gggcaccggc
     3361 gggcgcaact tccgcaccgt caccgtgacc gcggtgatcc gggaatctcc caacacgacc
     3421 tcggcgagcc gtgccgccat gcgttccatc agagcgaagc gctcgtcgac gatcaccgct
     3481 tcgacctgcg cacacagcgc gccgtagtcg acggtgtcgt cgagggcgtc ggaggctccg
     3541 gccgccgaca gatcggtctc gacgtcgagg tcgacctcga acggctggcg gcgctcctgc
     3601 tcttcgggaa gggcaccgca gaacccgagc gcccggaggc cccggagctg gatcacgtcg
     3661 gccacgggct actcggctgc cgcagcgttg gcgcgaatct tggccgcgac cgccacgatg
     3721 tcgcgacccg ccggaccgag ctgacgcgac gtgatgtgct cgagcaggaa gatcgcccgg
     3781 ggttgctcga cgacgatgcc cgcccacacg aggtagccgg ccatgatgcc gacgagccgg
     3841 tcgttgagct cttcgtggtg gtagagcacc ttggcgcccg agtcgagcag gccctgcagc
     3901 tcggggtaga tgtcgccgag gtagcgcggg tagtcctccg tcgccggcag cgggcggtgg
     3961 cgccaggtga ccgagagctc gtcgtagttg tgcaggttgt ggttggcggg gatcaggctg
     4021 atgaccggag cgaagccctg ctcgcggatc cagatgatct cctcctgacg gcgcaccttg
     4081 cggtggttgg tgccgtagcc gccggggcgc tcgcagatgg cgagcttgtc ctgcatcacc
     4141 cagctgaaat gccggggctt gatgccctgg gcccacttgc cttttgctgc catgttgtcc
     4201 tcgtcagttg cccgcgacgg cgggggtggg ggtgttcgaa cgggccgcgg cgacggcgag
     4261 cgcggcctcg acatggggtc gcacgtcgtg cgcccggatg atctgtgctc ctgccatcat
     4321 cgcccacgtg gcgagggcga cgcccatctc cagtcggtcc gaaggtggtg aaacgacgtc
     4381 ggcgccgagt ccgtcggccc gagccgtgag gactccgagc gacttcttgc ggctgacgcc
     4441 cagcaggacc ggggtgccgc gatcgaccag gtcgccgatg ccggccacga gttcgaggtt
     4501 gtggtcgagc gacttgccga acccgaagcc ggggtcgacc cacacctcgg ccacgccggc
     4561 cgaatgggcc gtggcggcgc gctcggtgag gaagtcgtac acctccgttc gagcgtcgac
     4621 gtaggtgggg tcgtcctgca tggagcacgg ctcgccctgc atgtgcatcg ccacccagcc
     4681 cacacccagg tcggcggcga cctgccacag cgaggcgctc acatcgttga tgacggtcgc
     4741 cccggccgcg accgcggccc gggcaacctc ggcgcgacgg gtgtcgacgg agatgcgggc
     4801 gccaaagccg tcgagggcgg tgatcgcctc gatcacgggg accacacggg ccagctcctc
     4861 gtcgatcgac accggggcag cgcccggtcg ggtcgactcg ccccccacgt cgacgaagga
     4921 cgctccctcg tccacaaggc ggcgcgcctg ggtcaccgcg gcgtcgagat cgacatagcg
     4981 gccgccgtcg ctgaacgagt cgggcgtgac gttcaggatg cccatgatct gggggatcac
     5041 cgccgccaat ggtagatcag ggtgtcaaat tgggatttgg gcgagcgccg ctcggccccg
     5101 tcagcgggtc acgaacgagg atggtgcgct tgcggtccca gtggggcgcg ctggagcacc
     5161 atcctggacc tctcggaacg gatcgttccg gtgtccggcc agcgtcgtcg gccgccgccg
     5221 ccgtcgctca cggcggctcc gggaatgctc agcgcagggc gaccaggccg cgtagatctc
     5281 cgtcctcgtc gacccagggc gcggcctcgc cgaagcca
//
LOCUS       DAOSOA010000233         5272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15755_length_5272_cov_7.712670, whole genome shotgun sequence.
ACCESSION   DAOSOA010000233 DAOSOA010000000
VERSION     DAOSOA010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15755_length_5272_cov_7.712670"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1609
                     /locus_tag="PKY13_12790"
     CDS             50..1609
                     /locus_tag="PKY13_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT80863.1"
                     /translation="MFPGDFAHTTPDKPAVVMAGTGETVTYAELDAEANRLSRVFRAA
                     GLQQGDHVAFCLENHPRFLSIMWGAHYAGLYYTAMSSRLTTDEMAYIVADCGAKAFIT
                     SAYKSDQAALLVDLMPGVELRLMLDATVDGYDSYEATIAAHEPTPLDEERVEGQDMLY
                     SSGTTGQPKGVKVPMLDAALGQGSDGVTMLCQLLFGAAEDTMYLSPAPLYHAAPLRFC
                     RAVQRSGGTVVVMEHFDAEQYLALVEQYRITFSQVVPTMFIKMLKLPDDVRAKYDLSS
                     LRTVVHAAAPCPVAAKRSMIEWWGPVVHEYYAGTEGNGFVYTNSEDWLAHPGTVGKAV
                     IGVLHIVGEDGDEVPVGEPGTIYFEGSGDAEPFQYHNDPEKTAGSRDPKGRGWSTLGD
                     VGRVDEDGFLYLTDRKAYMIITGGVNVYPQEAENLLALHPKVADVAVLGVPNDDFGEE
                     VKAVVQPVDMADAGPDLERELIAYCKERLADVKCPRSVDFREELPRHPTGKLYKRLLK
                     DEYWADAGRTI"
     gene            1631..3067
                     /locus_tag="PKY13_12795"
     CDS             1631..3067
                     /locus_tag="PKY13_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT80864.1"
                     /translation="MDAELSRRKRRVPAWWADAKLGIFIHWVPASVPGFAPTDVEIGD
                     LLTAREPAPLSRIPYSEWYENSLRFPDSPVSAFHRDTYGNRPYAEFADDFVAGLGSWD
                     PDDWARRFAATGARYVVLVTKHHDGWCLWPSAVSNPHRPGWFSERDLVGELATAVRAA
                     GMRFGVYYSGGYDWTFDATPIGSVAAGLEAIPGGDYPGYADRQVRELIDRYQPSLLWN
                     DIAWPAPRAPLEELFSYYFDRVPDGVVNDRWMPTTGVIRAAGHPRLRPLVDGVMRRSA
                     QKSGGLVPPTPPFFQYRTPEFTTFEEIQTTPWECVRGMDHGFGYNRTSTEADHLGHDD
                     LLQSLVDIAATGGNLLLNVGPRGEDATIPDAQLRRLDWMAEFSTTTAAALQGTRPWVR
                     PRATTPEGVEVRFTAHGDRVWALCWSPSGTMPGSITLPVRSTPTTSVTALDGAMLAAE
                     PAATGLLVATPASDLPIVAIALDHVVAT"
     gene            3064..3939
                     /locus_tag="PKY13_12800"
     CDS             3064..3939
                     /locus_tag="PKY13_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT80865.1"
                     /translation="MTAIPFLSEFDPAYGAVVHVAPGLRRVVAENPSKYTAWGTGTYL
                     VGEGDVAIVDPGPLLDAHVDALLAAVAGETVTHLLVTHTHADHSPAAAAVKELTGATT
                     YGFGPHPLDARSESEEHGDVVFVPDVEVRHGDVIEGPGFEFECLHTPGHISNHICYAE
                     RRRGILFPGDHVMGWSTSVISPPDGRVADYLDGLRLLLARGEAPFFPTHGPPITDPAP
                     YVQALLDHRLEREQQVVDLLSDGRRTVAELVAVMYADVRAELHEPAARSVGAHLVKLV
                     DEGRVTRHDDRYGLT"
     gene            complement(3946..4923)
                     /locus_tag="PKY13_12805"
     CDS             complement(3946..4923)
                     /locus_tag="PKY13_12805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HOT80866.1"
                     /translation="MQRVVCQQWGPPEDLEIVDLDDPHPAAGRVIIEVAAAGVNFVDA
                     LFVAGTYQIKVPPPFTPGSECAGTVVAVGDGVTTVSVGDRVLSSLGLGAWATHVEVPA
                     DAVTLVPDSLDLATAAALVQSYCTAWFALTRRTHVSAGESVLVLGAAGGVGLAGIDVA
                     SALGATVIAAASSPEKLADAVAKGASSTIAYETEDLKARARELSGGGVDVVIDPVGGR
                     HTEAALRSLRLGGRLLVIGFAAGDIPSLPTNQILLNNRSVVGIDWGAWAMGHPAEQAE
                     LLDEVLGAVADGRLHPSPPAQRSLHEAGTVLRELLDRRLRGKVVLTPTR"
     gene            4979..>5272
                     /locus_tag="PKY13_12810"
     CDS             4979..>5272
                     /locus_tag="PKY13_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80867.1"
                     /translation="MDPVDRSRLEANCADCFGLCCVALRFDVSAEFAIDKPAGEPCPN
                     LDDSFRCLIHRSLRDDGFSGCTTYDCFGAGQRISQETFGGVDWRRDPGSQDEMF"
BASE COUNT          788 a         1881 c         1818 g          785 t
ORIGIN      
        1 tcgcgcgtct gcagcgcgag cgccgatcgc gagtcgtaga ttgcggcgca tgttcccggg
       61 ggatttcgca cacaccacac cagacaagcc ggccgtcgtc atggcgggta ccggagagac
      121 cgtcacctac gccgagctcg acgccgaggc caaccgcctc tcccgcgtct tccgcgcggc
      181 ggggctgcag cagggcgacc acgtcgcgtt ctgcctcgag aaccacccga ggttcctgtc
      241 gatcatgtgg ggcgcccact acgcggggct ctactacacg gcgatgagct cgcggctcac
      301 gaccgacgag atggcgtaca tcgtcgccga ctgcggcgcg aaggccttca tcacgagtgc
      361 ctacaaatcc gaccaggcgg cactgctcgt cgacctcatg ccgggcgtcg aactgcgcct
      421 gatgctcgac gcgacggtcg acggttacga ctcctacgag gccacgatcg ccgcgcacga
      481 gccgaccccc ctcgacgagg agcgggtcga gggccaggac atgctctaca gctcgggcac
      541 gaccggccag ccgaagggcg tgaaggtgcc gatgctcgac gctgcgctcg gccagggttc
      601 cgatggcgtg acgatgctgt gccagctcct gttcggcgcg gccgaggaca ccatgtacct
      661 gtcgccggcc ccgctctatc acgcggcccc gctgcgcttc tgccgcgccg tgcagcggtc
      721 cggcggcacc gtcgtcgtca tggagcactt cgacgccgag cagtacctgg ccctcgtcga
      781 gcagtaccgc atcaccttca gccaggtcgt gccgaccatg ttcatcaaga tgctcaagct
      841 gcccgacgac gtccgcgcca agtacgacct gtcgtcgttg cgcaccgttg tgcacgccgc
      901 ggccccgtgc ccggtggcgg cgaagcgctc gatgatcgag tggtggggtc ccgtggtgca
      961 cgagtactac gccggcaccg agggcaacgg ctttgtgtac acgaacagtg aggactggct
     1021 ggcccatccc ggcaccgtcg gcaaggccgt catcggcgtg ctgcacatcg tcggcgagga
     1081 cggcgacgag gtgccggtcg gcgagccggg caccatctac ttcgagggtt cgggcgacgc
     1141 cgagccgttc caatatcaca acgaccccga gaagacggcg ggctcccgcg accccaaggg
     1201 ccggggttgg tcgacgctgg gcgatgtcgg acgcgtcgac gaggacgggt tcctctacct
     1261 caccgatcgc aaggcgtaca tgatcatcac gggcggggtg aacgtgtacc cccaggaggc
     1321 cgagaacctg ctcgcgctcc accccaaggt cgccgacgtg gccgtgctcg gtgtgccgaa
     1381 cgacgacttc ggcgaggagg tgaaggcggt ggtccagccg gtcgacatgg ccgacgccgg
     1441 ccccgatctc gagcgtgagc tgatcgcgta ctgcaaggag cggctggccg acgtgaagtg
     1501 cccccgttcg gtcgacttcc gcgaggagct cccgcgccac cccaccggca agctctacaa
     1561 gcgactgctg aaggacgagt actgggccga cgccggccgc accatctgag ccggtcggag
     1621 cacacccgtc atggacgcgg agctcagccg acgcaagcgg agggtgccgg cgtggtgggc
     1681 cgacgccaaa ctcggcatct tcatccactg ggtgccagcg tcggtcccgg ggttcgcccc
     1741 gaccgacgtc gagatcggcg acctcctcac cgcccgggag ccagcgcccc tgtcgcgcat
     1801 cccgtactcg gagtggtacg agaactccct gcggttcccc gacagtccgg tgtcggcgtt
     1861 ccatcgcgac acctacggca accggcccta cgccgagttc gcggacgact tcgtcgcggg
     1921 gctcggctcg tgggatcccg acgactgggc acggcgcttc gccgcgaccg gcgcccgcta
     1981 cgtcgtgctc gtgaccaagc accacgacgg ctggtgcctc tggccctccg ctgtgtcgaa
     2041 tccacaccgc cccggctggt tcagcgaacg cgacctcgtc ggggagctgg cgacggcggt
     2101 tcgggccgcc ggcatgcggt tcggcgtgta ctactccgga ggctacgact ggacgttcga
     2161 cgccacgccg atcggctcgg tcgcggccgg tctcgaggcg atccccggcg gcgactaccc
     2221 cggctacgcc gaccggcagg tgcgtgagct gatcgatcgc taccagccgt cgctcctctg
     2281 gaacgacatc gcctggccgg cgccccgagc accgctcgag gaactgttct cgtactactt
     2341 cgaccgcgtt cccgatggcg tcgtgaacga tcgctggatg ccgacgaccg gggtgatccg
     2401 cgccgccgga catccgcgac tgcgcccact ggtcgacggg gtgatgcgtc ggtcggcgca
     2461 gaagtcgggc gggctcgtgc ccccgacacc accgttcttc cagtaccgga cgcccgagtt
     2521 cacgacgttc gaggagatcc agacgacgcc ctgggagtgc gtccgtggca tggaccacgg
     2581 cttcgggtac aaccggacgt cgaccgaggc cgaccacctc ggacacgacg acctgctgca
     2641 gtcgctggtc gacatcgccg cgaccggcgg caacctgctg ctgaacgtgg gtccccgcgg
     2701 tgaggacgcg acgattcccg acgcccagct ccgccggctc gattggatgg ccgagttctc
     2761 gacgacgacc gcggcggcgt tgcagggcac ccgcccgtgg gtgcggcccc gcgccacgac
     2821 accggagggg gtggaggtgc ggttcacggc gcacggcgat cgggtgtggg cgctgtgctg
     2881 gtcgccctcg gggacgatgc cgggctcgat cacgctgccg gtgcgctcca cccccacgac
     2941 ctcggtcacg gcgctcgacg gcgcgatgct cgcggccgag ccggccgcca ccggcctcct
     3001 ggtcgccacg cccgcgagcg atctcccgat cgtggcgatc gcactcgacc acgtcgtcgc
     3061 gacgtgacag cgattccgtt cctctccgag ttcgaccccg cgtacggcgc ggtcgttcac
     3121 gtcgcccccg gcctccggcg tgtagtggcg gagaacccgt cgaagtacac ggcgtgggga
     3181 acgggcacgt acctcgtggg cgagggcgac gtcgcgatcg tcgaccccgg ccccctgctc
     3241 gacgcgcacg tcgacgccct cctcgcggcg gtcgccggtg agacggtcac ccacctcctc
     3301 gtcacccaca cccacgccga ccactcgccg gcggcggccg ccgtgaagga actgacgggc
     3361 gcgaccacct atgggttcgg cccgcatccg ctcgacgccc ggagcgagtc ggaggagcac
     3421 ggcgacgttg tgttcgtgcc cgatgtcgag gtccgacacg gcgacgtcat cgaagggccc
     3481 ggcttcgagt tcgagtgtct gcacactccg gggcacatct ccaaccacat ctgctacgcc
     3541 gaacggcgtc ggggcatcct gttcccgggc gatcacgtga tggggtggtc gacgtcggtc
     3601 atctcgccgc cggatggtcg cgtcgccgac tacctggacg gcctccggtt gctcctcgcc
     3661 cgcggcgagg cgccgttctt cccaacccac ggcccgccga tcaccgaccc ggccccctac
     3721 gtacaggcac ttctcgacca ccggctcgaa cgcgagcagc aggtcgtcga cctgctgtcg
     3781 gatggtcggc gcacggtggc cgagctcgtc gcggtgatgt acgccgacgt ccgcgccgag
     3841 ctccatgagc ccgccgcacg ttcggtgggc gcgcacctgg tgaagttggt cgacgagggt
     3901 cgggtgaccc gccacgacga ccgctacggg ctgacctgac cgacgtcacc gcgtcggggt
     3961 gagcaccacc ttgccgcgta gccggcgatc gagcagctcg cggaggaccg tgccggcctc
     4021 gtgaagggaa cgctgcgcgg gaggggacgg gtgcagccgt ccgtcggcga cggcgccgag
     4081 cacctcgtcg agcagctccg cctgctcggc cgggtgaccc atcgcccagg caccccagtc
     4141 gatgccgacc accgaccggt tgttgagcag gatctggttg gtcgggagcg acggaatgtc
     4201 gccggcggcg aagccgatga cgagcaggcg gcctccgagg cgaagcgagc gcaacgctgc
     4261 ctcggtgtgg cgtcctccga cgggatcgat cacgacgtcc accccgccgc ccgacagttc
     4321 gcgggctcgg gccttgaggt cctcggtctc gtacgcgatg gtgctgctcg cccccttggc
     4381 gacggcgtcg gcgagcttct cgggggacga ggcggcagcg atcacggtgg cccccagcgc
     4441 gctggccacg tcgatgccgg cgagcccgac gccgccggcg gcaccgagca cgagcaccga
     4501 ctcgccggcg ctgacgtgcg tgcggcgagt gagcgcgaac caggccgtgc agtagctctg
     4561 gacgagcgca gctgcggtcg cgaggtcgag ggagtcgggc acgagcgtca cggcgtcggc
     4621 cgggacctcg acgtgcgtcg cccacgcgcc gagaccgagc gacgacagga cacgatcacc
     4681 gaccgacacg gtcgtgacgc cgtcaccgac ggcgaccacc gtgccggcgc attcgctgcc
     4741 cggcgtgaag ggcggtggca ccttgatctg gtacgtgccg gcgacgaaca gggcgtcgac
     4801 gaagttcacc ccggccgccg ctacctcgat gatcactcgg ccggccgccg ggtgcggatc
     4861 gtcgaggtcg acgatctcca gatcctccgg cgggccccac tgctggcaga cgacgcgttg
     4921 catggcgcga ccctacggca gccgatccga cgggcgtgcc ggtaacgtcg cctgccgcat
     4981 ggaccctgtc gatcgctcac gactcgaggc gaactgcgcc gactgcttcg gtctgtgctg
     5041 tgtggccctg cggttcgacg tgtcggcgga gttcgcgatc gacaagccgg ccggggagcc
     5101 gtgcccgaac ctggacgatt cgttccgttg cctcatccat cggagtctcc gcgacgacgg
     5161 gttctcgggg tgcacgacct acgactgctt cggcgccggt cagcggatct cccaggagac
     5221 gttcggtggc gtcgattggc ggcgggaccc gggctcgcag gacgagatgt tc
//
LOCUS       DAOSOA010000234         5263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15797_length_5263_cov_5.619048, whole genome shotgun sequence.
ACCESSION   DAOSOA010000234 DAOSOA010000000
VERSION     DAOSOA010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15797_length_5263_cov_5.619048"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1010..3595
                     /locus_tag="PKY13_12815"
     CDS             1010..3595
                     /locus_tag="PKY13_12815"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOT80868.1"
                     /translation="MLCASDHRFAWSLKFGISGAPQGQDLTSSCQVGPFTLSMRTGTT
                     TPPPPNEPISGVPYNDETGRATVVDNSFSVPGATGCALLGAANGPLSAAFGVPAASGQ
                     NTAVLTLEATPKIAKGVKASNVPSVTTGVSPLTVNFNGTGSTAVKPIASYAWDFGNGE
                     TATGSTAFTTYATPGTYQAKLTVTDTDGDKDVSIKTITVNEPPNVVPTAAIGSSGTGG
                     VAPYAVNFDGTGSNDPDGSIVSYAWDFGNDRTATTALASANYTSPGTYTVTLSVTDNR
                     GAVGTATRVITVTGAPNTPPSAVIRTVSVAGTVPLTVNLSGTNSSDPDGSIASYAWDL
                     GNGATGTGPSIQAIYNAIGSYTVTLVVTDDRGAQATTTLVIEVSENFNFPPVADFVAD
                     PVTGSAPLSVSFDGTASSDEDGTIASYAWAFGNGQNGAGPTPAAVTYSIPGTYTAKLT
                     VTDNRGATGTATQTITVLPPPNQTPIANFTPTPTTGSAPLLVQLSSAGSSDADGAITG
                     YAWNFGNGTTSTSPNPSAVFNAAGTFTISLSVTDNSGASAVKSTTIVVNPANVPPVPV
                     ISATPITGSAPLTVNVNGAGSSDADGSIVSYAWDFGNGQTATGATASTVYSTQGSYTL
                     RLTVTDNRGSVRTTTTTIVAGTTNQRPVAVLTALPTSGPAPLVVTLKSLGSNDPDGSI
                     TKYSWDLGNGQTLLGSQVQFNYSIPGSYTVVLTVTDNRGATATATETVVVDPPVAVTD
                     RVRFQFGGAVNYTYDGKITGGSVSVTRDVFGLAGVSGAGTFTGPGNSPATYSVNLSRF
                     LWFNAFIGTVVVNDPANGVNNVTTSLFFAGLSSPSATSARATGSWNLNGAPYSFTFNF
                     DDRVS"
     gene            3685..4914
                     /locus_tag="PKY13_12820"
     CDS             3685..4914
                     /locus_tag="PKY13_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80869.1"
                     /translation="MTATACPGDTPSGGGGGTTVPASTTTLPGATTTTTVPVSTTTSI
                     PGGSTVPTLVAGRLHTCSLRPDSTVRCWGNNSFGQLGTGTLTSSTIPVKVPGLTGVTA
                     ISAGGFSTCALLADATVKCWGNNADGQLGDGSSTSSSTPVSVFNLSGVTAVAVGGSHT
                     CALKADTKVSCWGDNFSGQLGTGSNSPATTPRSVSGLTGVTSVSAGDAHTCALLTAGG
                     VTCWGDNVNGQLGNGTFTNSNVASTVTGLSGATSIVAGAGFSCADRSTGIPACWGENL
                     YGQLGNGAVLPPPPVQGDPPPPPMKENTPQNVVGLVGVSGTDAGDDHGCAVFAGNVAC
                     WGRNDSSQLGIAGGNTSNPTLVTGTANASQIAGGSSHTCALKTNGTAVCWGDNSSGQL
                     GTGNFTSATAPVSVIAL"
     gene            complement(4996..>5263)
                     /locus_tag="PKY13_12825"
     CDS             complement(4996..>5263)
                     /locus_tag="PKY13_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80870.1"
                     /translation="AQCVLLHEIPSQEQLAVRNDSDGRHPTVQVARTFHAMSSIYELK
                     LQILIRCGSRAAQLRRPTRRIPTERRVRGDSTGDWLIAMAHHQR"
BASE COUNT          847 a         1891 c         1651 g          874 t
ORIGIN      
        1 caccctcacc ccgagggccg gtgccgcctc ctacaccacg tcatgggact cactcagagg
       61 ctgcgagatc gctctgatga agatcggttc gtcagctcca cgctcggcgg gttctgattt
      121 gtagaccggc tctttcggag ccggtttcgg acggtctctg cctggacggc gaggcgtgcg
      181 ccgacgccgg cgagggaagt ggccatctgc gtggagggtc gcggcgatct ggagttcggt
      241 cgcggacgag cgctcgccgg agcggtgggc gagtcggcgt cgcgtgacgt tgccgatggc
      301 ggtcgtgcga ctgataatga accgcgctgc gatttcgttc acgagttcgc cggctggacg
      361 gtgattgaac gtcggccgga ctccgacgcg ggcgcggttc cctcgggaag gccggtcgca
      421 acccggcaag cttcggctgt gaccaagtgg ccgaagtcgt atgttgcgac catctccagc
      481 agggtctgcc cgttgttgcg gggcgaaggc gggacggccg attctgtggg gtatgccgtc
      541 ggctgacgtg gtcgaagcga tgaaccgcgg gggagatcaa cactgcgttc ggtgggtgct
      601 gtccgtccga ttaggaccca acccatcggt gcggcgagtg tggggttgag ctcggtcgac
      661 ggcgtcgcag tcgcaacgac gttccctcag ttgactccgt ttcggctgag gtggacccgc
      721 tgaaggccgc tgcgatggcc aacgccggcc tgctcgatcg cgggcaacga attcgggtgg
      781 tgttcatcgg caccatctcc agagttgtca tcgtggtccg tacgggctcc atccgtcgag
      841 gtacggaacg aggtcgctcg cggtgatcgt gtcgggaagc tccgggccac gcggcggcgc
      901 tcctcgcagc gggtcgatct gatccgcgta gccgcgtgac cattcgagcc agtcgctcac
      961 gtcgtcgtcg ctcgggtggt ttcgggctgc ggcgtcgcag aacgctcgaa tgctctgcgc
     1021 ctcggaccac cgtttcgctt ggtccctcaa gttcggcatc tccggtgcgc cccagggtca
     1081 ggacctcacg tcgtcgtgcc aggtgggccc cttcaccttg tcgatgcgga ccggtaccac
     1141 gaccccgccg ccgcccaacg agcccatcag cggtgtgccc tacaacgacg agaccggccg
     1201 cgccaccgtc gtcgacaact cgttctcggt tcccggagcc accggttgtg ccctcctcgg
     1261 tgcggccaac ggtccactga gcgccgcctt cggcgtgcct gcagcgtcgg gccagaacac
     1321 tgctgtcctg accctcgagg ccaccccgaa gatcgccaag ggtgtcaagg cctccaacgt
     1381 tccgtcggtc accaccggcg tctccccgct caccgtcaac ttcaacggca ccgggtcgac
     1441 cgcagtgaag ccgatcgcca gctacgcctg ggacttcggc aacggcgaaa cggcgaccgg
     1501 gtcgaccgcc ttcaccacgt acgccacgcc cggtacctac caggccaagc tgaccgtcac
     1561 cgacaccgat ggtgacaagg acgtgtccat caagacgatc acggtcaacg aaccgccgaa
     1621 cgtggtcccg accgctgcca tcggtagctc cggtaccggg ggtgtcgctc cgtacgccgt
     1681 gaacttcgac ggcaccggct cgaacgatcc cgacggctcg atcgtcagct acgcctggga
     1741 cttcgggaac gaccggaccg ccacgaccgc actcgcctcg gcgaactaca cctcgcccgg
     1801 cacctacacg gtgacgttga gtgtcaccga caaccgtggc gcagtcggca cggccacccg
     1861 ggtcatcacc gtgacgggtg cgcccaacac gccgccgagc gccgtcatcc gcacggtgtc
     1921 ggttgccggt accgtcccgc tcacggtcaa cctgtcgggc acgaactcgt cggatcccga
     1981 cggttcgatc gccagctacg cctgggacct cggcaacggt gccaccggca ccggccccag
     2041 tatccaggcc atctacaacg ccatcggctc gtacacggtg accctcgtcg tcaccgatga
     2101 ccggggcgcc caggcgacca ccaccctcgt catcgaggtg tcggagaact tcaacttccc
     2161 gccggtcgcc gacttcgtcg ccgatccggt caccggcagc gctccgttgt cggtgtcgtt
     2221 cgacggcacc gcatcgagtg acgaggacgg caccatcgcc agctacgcct gggccttcgg
     2281 caacggtcag aacggcgccg gtccgacgcc cgccgccgtc acctactcca tccccggcac
     2341 ctacaccgcc aagctgaccg tcaccgacaa ccgtggcgcc accggcacgg ccacgcagac
     2401 gatcacggtg ctgcctccgc cgaaccagac cccgatcgcc aacttcacgc cgacgcccac
     2461 cacgggcagc gccccgttgt tggtccagct gagcagcgcc ggctcgtccg acgccgacgg
     2521 cgccatcacc ggctacgcct ggaacttcgg caatggaacg acctcgacca gccccaaccc
     2581 gagtgcagtc ttcaacgccg ccggcacctt caccatctcg ctgagcgtca ccgacaactc
     2641 gggcgccagt gcggtgaaga gcacgaccat cgtcgtgaac cccgccaacg tgccgccggt
     2701 tcccgtgatc tcggcaacgc ccatcaccgg cagcgccccg ctcacggtca acgtcaacgg
     2761 cgccggctcg agtgatgctg acggctcgat cgtcagctac gcctgggact tcggcaatgg
     2821 gcagacggcc accggggcca ccgcctcgac ggtctactcg acgcagggct cgtacaccct
     2881 ccgtctcacc gtgaccgaca accggggctc ggtccgcacg acgacgacca ccatcgtcgc
     2941 cggcacgacc aaccagcggc cggtcgccgt cctgacggct ctgccgacct cgggcccggc
     3001 gcccctcgtc gtcaccctca agtcgttggg ctcgaacgac cccgacggct cgatcacgaa
     3061 gtactcgtgg gacctcggca acggccagac gctgctcggc tcgcaggtgc agttcaacta
     3121 ctcgattccc ggctcctaca cggtggtcct caccgtgacg gacaaccgtg gcgccactgc
     3181 cactgccacc gagaccgttg tggtcgaccc gccggtcgcc gtgacggacc gggtgcggtt
     3241 ccagttcggt ggagcagtca actacaccta cgacggcaag atcaccggtg gcagcgtgtc
     3301 cgtcacccgt gacgtgttcg gcctggccgg ggtgagcggt gcgggcacct tcaccggtcc
     3361 gggcaactcg ccggcgacct acagcgtcaa cctgagccgg ttcctctggt tcaacgcctt
     3421 catcggcacc gtggtcgtca acgacccggc gaacggtgtg aacaacgtga ccacctcgtt
     3481 gttcttcgcc ggcctgtcga gcccgtcggc cacctcggcc cgggcgactg gtagctggaa
     3541 cctcaatggc gctccgtact cgttcacctt caacttcgac gaccgggtga gctgatgacg
     3601 gaccccgttc tcggcgcttc ccaacggagt cgccgaacgc ttcgacgccg actctcgggt
     3661 gtggttgtcg gggtggtggt ggtcgtgacg gccaccgcct gccccggcga caccccgagc
     3721 ggcggtggcg gcggcaccac cgtccccgcg tcgaccacga cgctgccggg cgccaccacg
     3781 accacgacgg tcccggtgtc gaccaccacg tccatcccgg gtgggtcgac cgttccgacg
     3841 ctcgtcgccg ggcggctcca cacctgctcg ctccgcccgg acagcaccgt tcggtgctgg
     3901 ggcaacaaca gcttcgggca actcggtacc gggacgctca cctcgtcgac gatccccgtg
     3961 aaggttcccg gcctcaccgg cgtgaccgcg atctcggccg gtgggttctc cacgtgtgcc
     4021 cttctcgccg acgcgacggt gaagtgctgg ggtaacaacg ccgacggcca gctcggcgac
     4081 gggagctcga cctcgtcgtc aacgcccgtg tcggtgttca acctctccgg cgtcactgcc
     4141 gttgcggtcg gtggctccca cacctgtgcc ctcaaggccg acaccaaggt gtcgtgctgg
     4201 ggtgacaact tcagcggaca gctcggcacc ggttccaact cgccggcgac cacgccccgg
     4261 agtgtctccg gactcaccgg cgtgacctcg gtctcggcag gggacgcaca cacgtgcgcg
     4321 ctgctcacgg ctggcggcgt gacctgctgg ggcgacaacg tcaacgggca gctcggcaac
     4381 ggcacgttca ccaactcgaa cgtcgccagc accgtcaccg gtctcagcgg tgcgacctcg
     4441 atcgtcgccg gcgcaggatt ctcctgtgcc gatcggtcga cgggtattcc ggcctgctgg
     4501 ggtgagaacc tctacggcca gctgggcaac ggcgccgtcc tgcctcctcc gccggtccag
     4561 ggcgatccgc ccccgccgcc gatgaaggag aacaccccgc agaacgtcgt cggtctggtc
     4621 ggcgtgtccg gcaccgacgc cggcgatgac cacggctgcg ccgtgttcgc cggcaacgtc
     4681 gcctgctggg gacgcaacga cagcagccag ctcggcatcg ccggtggcaa cacctcgaac
     4741 ccgacgctgg tgaccggcac ggcgaacgcg agtcagatcg ccggcggctc gagccacacc
     4801 tgtgcgctca agaccaacgg caccgcagtc tgctggggtg acaactcctc cggccagctc
     4861 gggaccggca acttcaccag cgccacggcg ccggtgtcgg tgattgcgct ctaacggtca
     4921 gcatccttct cgtccagaac tccatatagg aatccctctt tccagctgaa tggaaagccg
     4981 tcgtcagggt tgtccctacc tctgatgatg agccatggcg atcagccagt cgccggtcga
     5041 atccccacga actcgccgtt ctgtggggat tcgacgcgtc gggcggcgca gttgggcggc
     5101 gcgggagccg caccggatca aaatctgtaa tttcaattcg taaatgctcg acattgcatg
     5161 aaatgtgcgt gctacttgca cggtcgggtg tcgtccgtcg gaatcattgc ggaccgccag
     5221 ttgttcctgg gaggggattt cgtgaagaag cacacattgc gcc
//
LOCUS       DAOSOA010000235         5256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15829_length_5256_cov_5.382042, whole genome shotgun sequence.
ACCESSION   DAOSOA010000235 DAOSOA010000000
VERSION     DAOSOA010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15829_length_5256_cov_5.382042"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /gene="dacB"
                     /locus_tag="PKY13_12830"
     CDS             <1..1092
                     /gene="dacB"
                     /locus_tag="PKY13_12830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOT80871.1"
                     /translation="TALDVLGPDATFTTRLAATSAPAGGVIEGDLWMIGGGDPVIDSD
                     TYQSTLKYGKTPHTRIEDIADKIVAAGVTQVTGSVKGDDRHFDDVRTIPTWPSRYLAQ
                     NQVGPLSALSVNDARTYGVLPGASSGTPQPASDPAAYAADALTQLLVARGVSVGGPAA
                     SGEAPDGLATVVDVPSLPLRDIIKEMLTFSDNNTAELLLKAVGSATSNEGTTAAGLRE
                     AQRVLTEHDIDMNGIVLLDGSGLDTGNRLTCRLLDDVLGTSGPSGPIADGLPVADGPD
                     GTLRDRFTKSPAAGAVRAKTGTLKAVTALSGWVTTTKGADVRFSTILNPSGRDVSASD
                     LAFETKVAEAILSYPDAVDPASVSPEASA"
     gene            1141..1752
                     /locus_tag="PKY13_12835"
     CDS             1141..1752
                     /locus_tag="PKY13_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOT80872.1"
                     /translation="MSTETPMFPLGSVLLPSMLLPLHIFEDRYRLMIEHVLAGDGTFG
                     VTMIERGSEVGGHDVRAAVGCTARVLDAEQQPDGRWHLVAVGTERISVDEWLPDDPFP
                     RAMVSSLPDTATTVTAWDELVTSFRSLIELVERVTGSTDLAPGVPMADDPAMATFEMA
                     AVAPIGPLDRYRVLSSTDVDARRLLLLDAFEHATVLVNDAPSN"
     gene            1762..2106
                     /locus_tag="PKY13_12840"
     CDS             1762..2106
                     /locus_tag="PKY13_12840"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOT80873.1"
                     /translation="MAQDRVSDKKGPAEHVAELKDLVVGYARQETWDPLKTLGRYVGF
                     GVGGGVAIGLGCVFLLLGLLRGLQSWDALDGTGAMSLIPFAATVLAAAVMIGLAVFGI
                     TKDDTKKKETTS"
     gene            2103..2309
                     /locus_tag="PKY13_12845"
     CDS             2103..2309
                     /locus_tag="PKY13_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80874.1"
                     /translation="MSADKITPNDIKAKLADIQGEATSTVEGAKNQIIAVGAGVAILL
                     LIIAFVLGRRGGAHRSTIIEVKRA"
     gene            2315..2842
                     /locus_tag="PKY13_12850"
     CDS             2315..2842
                     /locus_tag="PKY13_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80875.1"
                     /translation="MWRRLRELGLVRVFGRGPAALVAGSAVNWLIDQARESRQPERLD
                     TARLVPGETYLVTTRPGMNRAERRDSKKLRASSAKLTKQRTPNRKARTTASKLATAQK
                     KVAKATPGSKRAVRNERKVEVLGTRFDRLTAPTRQQRKLEVQVAELSARLETHRATAL
                     AKTAKRHHPRTKTFL"
     gene            2922..3122
                     /locus_tag="PKY13_12855"
     CDS             2922..3122
                     /locus_tag="PKY13_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOT80876.1"
                     /translation="MIVKLRHPTREIEVPGPVSVIKLLNRLDLNRESVLLVRNGELVP
                     GDSMLDDADEIEIRPVISGGFA"
     gene            3133..4116
                     /locus_tag="PKY13_12860"
     CDS             3133..4116
                     /locus_tag="PKY13_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT80877.1"
                     /translation="MKCTVCREPAVIDLRRHNSNFCAPHFTKFCRDQTQKAIADFDMM
                     STDDKVLVAVSGGKDSLAVWDILLDLGYQASGLYIGLGIGEYSDNSKQFAVDFAERRG
                     AELIQIDLPAEYGYDIPEGSRAAKRAPCSACGLSKRHLFDQAAVDGGFDVLVTGHNLD
                     DEAAVLFGNVLHWQTEYLGRQHPVLPARHGFPRKVKPLVRLGEREMAAYCIVTGIDYI
                     VEECPMAVGNKHIGYKNALNEIEKQSPGSKADFYLGFLRNAADRFAGEAAEGREALGA
                     CVRCGSPSPAEVCAFCRLVERATADRNPAPTPQSVELGPARRTATNKDATT"
     gene            4113..4889
                     /locus_tag="PKY13_12865"
     CDS             4113..4889
                     /locus_tag="PKY13_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOT80878.1"
                     /translation="MTAELQEGELVLLLDSKQRQYLVTLQSGKEFHSHAGYISHDEIL
                     GAGEGVTLRSTRGASYTVLRPTLSEFILKMPRGAQVIYPKDIGPILMMADIGPGLKVL
                     ESGVGSGALSMGMLRCGVDIVGYELREEFADRARQNVEKFLGPEALSRYRVELRDCYE
                     GIDETDLDRVILDLPEPWQVVPHAATALRPGGLILGYSPSIVQVMQFRDALDKAGFIL
                     AESLEVLNRSWHIEGNAVRPDHRMVAHTGFLTTARRGSDQ"
     gene            4906..>5256
                     /locus_tag="PKY13_12870"
     CDS             4906..>5256
                     /locus_tag="PKY13_12870"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOT80879.1"
                     /translation="MNELDLIFLLGALVAGLGGWRFGFVRRVIGWIGLVIGVVVASKL
                     LPVVFPTPETPEPADLIRSLVVILGAGLIGQAVGHVVGGRMKGMLDIARFGLIDSAGG
                     AVLGVAGVVITAWMF"
BASE COUNT          829 a         1797 c         1821 g          809 t
ORIGIN      
        1 accgcgctcg acgtgctcgg gcccgatgcc acgttcacga cccgcctggc cgccacgtcc
       61 gccccggcgg ggggagtgat cgagggtgac ctctggatga tcggtggcgg cgacccggtc
      121 atcgacagcg acacgtacca gtcgaccctc aagtacggga agacgcccca cacccggatc
      181 gaggacatcg ccgacaagat cgtcgccgcc ggcgtcacgc aggtgacggg gtcggtgaag
      241 ggcgacgacc gacacttcga cgacgtccgt acgatcccga cgtggccgtc gcggtacctg
      301 gcccagaacc aggtcgggcc gctgtcggcc ctgtcggtca acgacgcccg cacctacggc
      361 gtcctcccgg gcgcttcgtc gggaacgccc cagccggcgt cggatccggc cgcctatgcc
      421 gccgacgccc tcacgcagct gctcgtcgcc cgtggtgtct cagtgggcgg cccggcggcg
      481 tcgggggagg cgcccgacgg gctggcgacg gtcgtcgacg ttccgtccct cccgttgcgc
      541 gacatcatca aggagatgct gacgttcagc gacaacaaca cggccgagct cctgctcaag
      601 gcggtaggtt cggcgacgtc gaacgagggc accacggcag cgggcctgag ggaggcgcag
      661 cgggtgttga ccgagcacga catcgacatg aacggcatcg tcctgctcga cggcagtggc
      721 ctcgacaccg ggaaccgact gacgtgccgc ctgctcgacg acgtgctggg cacgagtggc
      781 ccgtcggggc cgatcgccga tggcctgccc gtcgccgacg ggcccgacgg cacccttcgc
      841 gaccggttca cgaagagccc ggccgccggg gccgtgcgcg ccaagacggg cacgctcaag
      901 gccgtcaccg cgttgtcggg atgggtgacg acgaccaagg gtgccgatgt tcggttcagc
      961 acgatcctca atccgagcgg gcgcgacgtg tcggcctcgg acctggcctt cgagacgaag
     1021 gtcgccgagg cgatcctctc gtaccccgac gccgtcgacc ccgcgtcggt ctcgccggag
     1081 gcgagcgcat gagcactgcg ccggaggcga gcgcatgagc accgcgccgg aggcgagcgc
     1141 atgagcaccg agacaccgat gttcccgctg gggtcggtgc tcctgccgtc gatgctgctc
     1201 ccgctgcaca tcttcgagga tcgctaccgg ctgatgatcg agcacgtcct cgccggcgac
     1261 gggaccttcg gggtcacgat gatcgaacgt ggctcggagg tgggtggtca cgacgtccga
     1321 gcggcggtcg ggtgcacggc gcgggtgctc gacgccgagc agcagcccga cggccggtgg
     1381 cacctggtcg cggtcggcac ggagcgcatc agcgtcgacg agtggcttcc cgacgacccg
     1441 ttcccgcggg cgatggtctc gtcgctgccc gacacggcga cgaccgtcac ggcgtgggac
     1501 gaactcgtga cgtcgttccg cagcctcatc gagctcgtcg agcgcgtcac cggcagcacc
     1561 gacctggccc cgggggtccc catggccgac gatccggcga tggcgacgtt cgagatggcg
     1621 gcggtcgcgc cgatcgggcc gctcgaccgg taccgggtgc tgtcgtcgac cgacgtcgac
     1681 gcgcggcggc tcctgctgct cgatgccttc gagcacgcca cagttcttgt gaacgacgcg
     1741 ccgagcaact aggaagggcc gatggcgcag gacagagttt cggacaagaa gggtccggcg
     1801 gagcacgtcg ccgagctgaa ggatctcgtc gtcggctacg ccaggcagga gacgtgggac
     1861 ccgctcaaga cgctcggtcg ctacgtgggc ttcggtgtcg gcggcggtgt cgcgatcgga
     1921 ctcgggtgcg tcttcctgtt gctcggcctg ctgcggggcc tgcagtcgtg ggacgccctc
     1981 gacggcaccg gcgcgatgtc gctgattccg ttcgcggcga ccgtgctcgc cgccgccgtc
     2041 atgatcggtc tcgccgtctt cggcatcacc aaggacgaca cgaagaagaa ggaaacgacg
     2101 tcatgagcgc cgacaagatc acccccaacg acatcaaggc gaagctcgcc gacatccagg
     2161 gcgaggccac gtccaccgtc gagggcgcca agaaccagat catcgctgtc ggtgcggggg
     2221 tggcgatcct gctcctgatc attgccttcg tgctcggccg gcgtggcggg gcgcaccgga
     2281 gcaccatcat cgaagtgaag cgggcctagc cgtcgtgtgg cgtcgtcttc gcgagctggg
     2341 gttggtccgc gtcttcggga gaggtccggc agcgctcgtc gccggcagcg cggtcaactg
     2401 gctgatcgac caggcgcggg agtcccgcca gcccgagcgt ctcgatacgg cgcgcctcgt
     2461 tcccggtgag acctacctcg tcacgacccg ccccggcatg aaccgcgccg agcggcgtga
     2521 ctccaagaag ctgcgggcgt cgtcggccaa gctgaccaag cagcgcacgc ccaaccgcaa
     2581 agcccggacg acggcgtcga agctggcgac ggcacagaag aaggtggcga aggccacgcc
     2641 ggggtcgaag cgcgcagtac gcaacgaacg caaggtcgag gtgctcggca cccggttcga
     2701 ccgcctgacg gcgccgacgc ggcaacagcg caaactcgag gtgcaggtcg ccgagctcag
     2761 cgcccgcctc gagacccacc gggccacggc cctggcgaag acggccaagc ggcaccaccc
     2821 tcgaaccaag acgttcttgt agcggttgag cggtccgctc cggccgggcc gcccggtcag
     2881 cggttcacgc accggccgca gcatcggcca gactgttggg cgtgatcgtc aagctccgtc
     2941 atcccacccg cgagatcgag gttccgggcc ccgtcagtgt catcaagctg ctcaaccggc
     3001 tggatctcaa ccgcgagtcg gtcctgctcg tgcgcaacgg tgagctcgtg ccgggtgact
     3061 cgatgctcga cgacgccgat gagatcgaga tccgcccggt catctcgggt ggctttgcat
     3121 gaggatccag ccatgaagtg cacggtgtgt cgcgagcccg ccgtcatcga cctccggcgg
     3181 cacaacagca acttctgcgc gccccacttc acgaagttct gtcgtgacca gacccagaag
     3241 gcgattgccg acttcgacat gatgtcgacc gacgacaagg tgctcgtcgc cgtgtcgggc
     3301 ggcaaggact cccttgccgt gtgggacatc ctgctcgacc tcggctacca ggcgtcgggc
     3361 ctgtacatcg gtctcgggat cggcgagtac agcgacaact cgaagcagtt cgcggtggat
     3421 ttcgccgagc ggcgcggcgc cgagctgatc cagatcgatc tgccggccga gtacggctac
     3481 gacatccccg aggggtcacg ggcagccaag cgcgccccgt gctcggcgtg cggcctgtcc
     3541 aagcgtcacc tcttcgacca ggccgcggtc gacggtggct tcgacgtgct ggtcacgggc
     3601 cacaacctcg acgacgaggc ggccgtgctg ttcggcaacg tgttgcactg gcagaccgag
     3661 tacctcggcc gtcagcatcc tgtcctgccg gcgcggcacg ggttcccccg caaggtgaag
     3721 ccgctcgtgc ggctcggcga gcgtgagatg gcggcgtact gcatcgtcac gggcatcgac
     3781 tacatcgtcg aggaatgccc gatggcggtg ggcaacaagc acatcggtta caagaacgcg
     3841 ctgaacgaga tcgagaagca gtccccgggc agcaaggccg acttctacct cgggttcctc
     3901 cgcaacgccg ccgaccggtt cgccggcgag gccgccgagg gtcgcgaagc gctcggtgcg
     3961 tgcgtacgct gcggttcccc cagtcccgcc gaggtgtgcg cgttctgccg gctcgtggaa
     4021 cgggccaccg ccgaccgcaa ccccgcgccg acacctcaat ccgtcgaact gggtccggcc
     4081 cgccggaccg ctaccaacaa ggacgccacc acgtgaccgc cgaactccag gaaggcgagc
     4141 tggtcctcct gctcgactcg aaacagcgcc agtacctggt gacgttgcag tccggcaagg
     4201 agttccactc ccacgcgggg tacatctccc acgacgagat cctcggtgcc ggcgagggcg
     4261 tcacgctgcg gtcgacccgg ggcgcgtcct acacggtgct gcgcccgacg ctgagcgagt
     4321 tcatcctcaa gatgccccgc ggcgcccagg tgatctaccc gaaggacatc gggcccatct
     4381 tgatgatggc cgacatcgga cccggtctga aggtgctcga gtcgggggtc ggatcgggcg
     4441 ccctgtcgat gggcatgctg cggtgtggcg tcgacatcgt cggctacgag ttgcgcgagg
     4501 agttcgcgga tcgggcccgc cagaacgtcg agaagttcct cgggcccgag gcgctgagcc
     4561 ggtaccgggt cgagttgcgc gactgctacg agggcatcga cgagaccgac ctcgaccggg
     4621 tgatcctcga tctgcccgag ccgtggcagg tggtcccgca cgccgcgacg gcgctgcggc
     4681 ccggtggtct gatcctcggg tactcgccga gcatcgtgca ggtgatgcag ttccgcgatg
     4741 ccctcgacaa ggccgggttc atcctggcgg agtcgctcga ggtgctgaac cggagctggc
     4801 acatcgaggg caacgccgtg cgtcccgacc atcgcatggt cgcccacacc gggttcctca
     4861 cgaccgctcg tcgcggcagc gaccagtagc tgatcgcagc tgcgggtgaa cgagctcgac
     4921 ctcatcttcc tgctcggtgc gctcgtcgcc gggttgggag gatggcggtt cgggttcgtt
     4981 cggcgcgtca tcggttggat cggtctcgtg atcggcgtgg tcgtcgcgtc gaagctcctc
     5041 cccgtcgtct tccccacgcc ggagactccc gagcccgccg acctcatccg gagcctcgtc
     5101 gtcattctcg gcgccggcct gatcggccag gcggtcgggc acgtcgtcgg cgggcgcatg
     5161 aagggcatgc tcgatatcgc ccggttcggt ttgatcgact ctgccggggg tgcggtgctc
     5221 ggcgtcgccg gggtggtgat caccgcgtgg atgttc
//
LOCUS       DAOSOA010000236         5228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15934_length_5228_cov_4.491011, whole genome shotgun sequence.
ACCESSION   DAOSOA010000236 DAOSOA010000000
VERSION     DAOSOA010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15934_length_5228_cov_4.491011"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKY13_12875"
     CDS             <1..777
                     /locus_tag="PKY13_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT80880.1"
                     /translation="SADDGALIATILTDIRARRAREARDAEKQTPPTDATDDGADVSP
                     LTPRLEALAAREAADAAPTYSELQAQDLLELFLAGAGSNRPGRVGVYLHIDLDDLNAP
                     ADDGIEDVIAGRRSAHTGAGLDITDETLWGLMAKADITPIFNRNGRSLWYGRTRRLAP
                     DVLRRVLAHRDRRCRVPGCNRPPIHCEAHHVDHWPDGGTTDPKTCALACGYHHRSHHR
                     GDLGIAGDADGVLEFTRRDGSPLDVEPRYQRRSREEKRAS"
     gene            complement(774..1004)
                     /gene="secG"
                     /locus_tag="PKY13_12880"
     CDS             complement(774..1004)
                     /gene="secG"
                     /locus_tag="PKY13_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOT80881.1"
                     /translation="MYTVSLVLQFILALAVVFLVLIHSGKGGGLSDMFGGGLGAAASG
                     STVMEKNLDRITVIVALVFVFNSVILALLMDR"
     gene            complement(1030..1803)
                     /gene="tpiA"
                     /locus_tag="PKY13_12885"
     CDS             complement(1030..1803)
                     /gene="tpiA"
                     /locus_tag="PKY13_12885"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOT80882.1"
                     /translation="MADKRVPLISGNWKMHNNHLEALTLVQKLSYALGGHDYDKVHVS
                     VHPPFTDIRTVQTAVESDRMKLILGAQNCHFEEKGAFTGEVSPSMLAKLNVVYVIVGH
                     SERREIFGETDEMVNSKVKAVIKAGMTPILCCGETQEEREGGHAESKVDGQIRAGLAK
                     VTADQVGAMVIAYEPIWAIGTGLTATPEDAQNMCAHIRKVVGEVAGAEAAAKVRIQYG
                     GSVKPGNAAELMGQPDIDGALVGGASLDADDFARIVQYR"
     gene            complement(1796..2923)
                     /locus_tag="PKY13_12890"
     CDS             complement(1796..2923)
                     /locus_tag="PKY13_12890"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT80883.1"
                     /translation="MQFGVPELEDLPDVSGKSVLVRVDFNVPIKDGVIGDDLRIRAAL
                     PTLEWLTSHGAKVTCCTHLGRPKGAPDPAFSVAPVRARLAELAPGVELLENLRFNPGE
                     TTNDDAFVQELIAGHDLYVNDAFGASHRAHASIVGPPKFLPSAAGRLLAKEVEVLVGL
                     RDDPARPFVAITGGSKVSDKLGVIEALLDIADHLIIGGGMCFTFLSAMGHSIGASLFE
                     AEQVDNCKRLLDSHGDRLHLPTDIVALSPDGKLFSDQGEVRHVGRDVPEGWMGVDIGP
                     ESAGTFSDIILEGRTILWNGPMGVFEDPRFEAGTKVVAEAVAESRGFSVIGGGDSAAA
                     VAQFGLASEVDHVSTGGGASLELIEQGDLPGLEALRSAANG"
     gene            complement(2943..3956)
                     /gene="gap"
                     /locus_tag="PKY13_12895"
     CDS             complement(2943..3956)
                     /gene="gap"
                     /locus_tag="PKY13_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT80884.1"
                     /translation="MSVRVGINGFGRIGRNFFRAAKLRGVDIDFVAVNDLGSLKTMAH
                     LLKRDSVGGTFPGTIEAVDGGISVDGDVLKVLSERNPADLPWGDLGVDVVIESTGFFT
                     KRDTAAAHIDGGAPRVIISAPAEGVDATFVVGVNDDTFDPAKHIVVSNASCTTNCFVP
                     MIKVLDDAFGVEKGLMTTTHAYTGDQSLVDGPHSDLRRARAAAINIVPTSTGAARATG
                     LVLEAMKGKLDGTSLRVPIPTGSITDFTGVLKRDVTVEEINEAYKAAAAEGRLKGILD
                     YTDGEPIVSSDIVGSTFSSIIDSDMTMAMGNLAKVFSWYDNEWGYSNRLVDLALIVGA
                     ANQ"
     assembly_gap    3976..4075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4142..5017)
                     /locus_tag="PKY13_12900"
     CDS             complement(4142..5017)
                     /locus_tag="PKY13_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOT80885.1"
                     /translation="MIVAVGGGHGLATTLRALRRITDDVTAVVSVADDGGSTGRLRRS
                     TAQAAPGDLRKCLVALAEPNSALARSMEFRFDEGELDGHALGNLLLSAMSEVCGGLTS
                     ALDELGKLLGVRGRVLPATVEPVDLVALTVDGRRVRGQVAVMGTSGIDRVSLSPADAH
                     VAPDVLERIDAAELIVLGPGSLYTSVLAATAVPAVTEAIGRSSAHLVYVCNLRPQEGE
                     TAGYGVADHVRALHRHGLHPDTVLFDPSLDSGEGVPGGVPARLAAADSNVHDPHLLEQ
                     ALRAQLALRGPNLIP"
     gene            complement(5014..>5228)
                     /locus_tag="PKY13_12905"
     CDS             complement(5014..>5228)
                     /locus_tag="PKY13_12905"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOT80886.1"
                     /translation="LDETEPFLDRLSSLLEMLLPAYRQEGKAYLTIALGCTGGRHRSV
                     AMAEAVADRIKAFGVDARIHHRDVEK"
BASE COUNT          862 a         1763 c         1711 g          792 t
ORIGIN      
        1 tccgccgacg acggagcgct catcgcaacg atcctcaccg acatccgggc ccgccgcgct
       61 cgtgaggctc gtgacgccga gaagcagacg ccgcccaccg atgcgactga cgacggtgcc
      121 gacgtcagtc ccctgactcc ccgcctcgag gcgctggcgg cacgagaagc cgccgatgcc
      181 gcgcccacct actccgagct gcaagcgcag gatctcctcg agctgttcct ggccggcgca
      241 gggtccaatc gtccgggtcg ggtcggcgtc tacctccaca tcgacctcga cgacctcaat
      301 gcgcccgccg acgacgggat tgaagacgtc atcgctggcc gacgttcggc ccacaccggg
      361 gccgggctcg acatcaccga cgagacgctc tggggcctca tggccaaggc cgacatcacg
      421 ccgatcttca accggaacgg cagatcgctc tggtacgggc ggacccgccg cctcgctccc
      481 gacgtgctcc gccgggtgct cgcccatcgc gaccgacgat gtcgggtgcc gggatgcaac
      541 cggcccccga tccattgcga agcccatcac gtcgaccact ggcccgacgg gggtaccacc
      601 gaccccaaaa cctgcgcact tgcgtgcggg taccaccatc gcagtcatca ccggggcgat
      661 ctgggtatcg ccggcgatgc cgacggagtc ctcgaattca ctcgccggga cggatcccca
      721 ctcgatgtcg agccccgcta ccagcggcgg tctcgggaag agaaacgcgc gagctaacgg
      781 tccatgagca gggcgaggat caccgagttg aacacgaaga ccagcgccac gatgaccgtg
      841 atgcgatcga ggttcttctc catgaccgtc gaacccgagg ccgcagcacc gagtccgccg
      901 ccgaacatgt ccgacaggcc gccacccttg ccgctgtgga tcagcaccag gaagaccacc
      961 gccagggcga ggatgaactg cagaacgagc gagaccgtgt acataacggg cgacagccta
     1021 ccgctcgcgc tatcggtact gcacgatgcg tgcgaagtcg tcggcgtcga gcgacgcgcc
     1081 gccgacgagg gcaccgtcga tgtcgggctg gcccatgagc tcggcagcat tgccgggctt
     1141 gaccgagccg ccgtactgga tgcgcacctt ggcagccgcc tcggcgccgg cgacctcgcc
     1201 gacgaccttg cggatgtggg cgcacatgtt ctgggcgtcc tccggagtgg cggtgaggcc
     1261 cgtgccgatg gcccagatgg gctcgtaggc gatgaccatg gcaccgacct ggtcggcggt
     1321 caccttggcg aggccggcgc gaatctgccc gtcgaccttg ctctcggcat gtccgccctc
     1381 gcgctcctcc tgcgtttcac cacagcagag gatcggggtc atgccggcct tgatgaccgc
     1441 cttgaccttc gagttcacca tctcgtcggt ttcgccgaag atctcgcggc gctcggagtg
     1501 cccgacgatc acgtacacaa cgttgagctt ggcgagcatc gacggcgaca cctcgccggt
     1561 gaacgcgccc ttctcctcga agtggcagtt ctgcgcaccc aggatcagct tcatacgatc
     1621 ggactcgacg gcagtctgca ccgtgcggat gtcggtgaac ggcgggtgca ccgacacgtg
     1681 caccttgtcg tagtcatggc caccgagcgc gtagctgagc ttctgcacca gcgtgagcgc
     1741 ctcgaggtgg ttgttgtgca tcttccagtt gccgctgatg agcggcaccc tcttgtcagc
     1801 cattcgcagc actcctcaac gcctcaaggc cggggaggtc cccctgctcg atcaactcca
     1861 gactcgcgcc accaccggtc gacacgtggt cgacctcgct ggcgagcccg aactgcgcca
     1921 ccgcggcggc gctgtcacca ccgccgatga ccgagaagcc ccggctctcg gcgacggcct
     1981 cggccacgac cttcgtgccg gcctcgaacc gcgggtcctc gaacacaccc atcgggccgt
     2041 tccacaggat ggtgcggccc tcgaggatga tgtcggagaa cgtgccggcc gactcggggc
     2101 cgatgtcgac gcccatccaa ccctcgggaa cgtcgcggcc gacgtggcgc acctcgccct
     2161 ggtcactgaa gagcttgccg tcgggcgaga gggccacgat gtcggtgggc aggtgcaacc
     2221 ggtcgccgtg gctgtcgagc aggcgcttgc agttgtcgac ctgctcggcc tcgaacaacg
     2281 acgcgccgat cgagtggccc atggccgaca ggaacgtgaa gcacatgccg ccaccgatga
     2341 tcagatggtc ggcgatgtcg agcagggcct cgatgacccc gagcttgtcg gacaccttcg
     2401 agccgccggt gatggcaacg aacgggcggg ccgggtcgtc gcggagccca acgagcacct
     2461 cgacctcctt ggccagcaac cggccggccg ctgacggcag gaacttgggc ggcccgacga
     2521 tcgaggcgtg ggcgcggtgc gacgcaccga aggcgtcgtt cacgtacagg tcgtgcccgg
     2581 cgatgagctc ctgcacgaac gcgtcgtcgt tcgtggtctc ccccgggttg aaccggaggt
     2641 tctcgagcag ctcgacaccg ggagccaact cggccagccg cgcccgcacg ggcgctacgg
     2701 agaacgccgg gtcgggcgcg cccttcggac gacccaagtg ggtgcagcac gtgaccttcg
     2761 ccccgtgcga cgtcaaccac tccaaggtgg gcaacgccgc acggatgcgg agatcgtctc
     2821 cgatgacccc atctttgatg gggacgttga agtcgacacg gacgagaacc gacttgcccg
     2881 agacgtcggg caggtcttcg agctccggga cgccgaactg cactgtgttg gtcctgaccg
     2941 ggttactggt tcgcggcgcc gacgatgagg gcgagatcca cgaggcggtt cgagtagccc
     3001 cactcgttgt cgtaccacga gaagaccttg gccaggttgc ccatcgccat ggtcatgtcg
     3061 gagtcgatga tcgagctgaa ggtcgaaccc acgatgtcgg agctgacgat cggctcaccg
     3121 tcggtgtagt cgaggatgcc cttgagacga ccctcggcag cggcggcctt gtaggcctcg
     3181 ttgatctcct cgaccgtgac gtctcgcttg agcacaccgg tgaagtcggt gatcgaaccg
     3241 gtggggatcg gcacacgcag cgatgtgccg tcgagcttgc ccttcatcgc ctcgagcacg
     3301 aggccggtcg cacgggcggc gcccgtcgac gtgggcacga tgttgatggc ggcggcgcgg
     3361 gcacgacgga gatcgctgtg gggaccgtcg acgaggctct ggtcgccggt gtaggcgtgc
     3421 gtcgtggtca tgagaccctt ctcgacaccg aacgcatcgt cgagcacctt gatcatcggc
     3481 acgaagcagt tggtcgtgca cgaggcgttc gacacgacga tgtgcttggc cgggtcgaac
     3541 gtgtcgtcgt tgacgccgac cacgaacgtg gcatcgacgc cctcggcggg ggccgagatg
     3601 atgacgcggg gcgcgccacc gtcgatgtgg gcggcagcgg tgtcacgctt ggtgaagaag
     3661 ccagtcgact cgatgaccac gtcgacgccg aggtcgcccc aggggagatc ggcggggttg
     3721 cgctccgaca gcaccttcag gacgtcgccg tccaccgaga tgccaccgtc gacggcctcg
     3781 atcgtgcccg ggaaggtgcc gccgaccgag tcgcgcttga ggaggtgggc catcgtcttc
     3841 agcgagccga ggtcgttgac cgcgacgaag tcgatgtcga cacctcgaag cttcgccgcc
     3901 cggaagaagt tgcggccgat ccgcccgaag ccgttgatac ccacacgaac gctcattgct
     3961 ctcctcgtca ttgagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgcc
     4081 cgaagccgtt gatacccaca cgaacgctca ttgctctcct cgtcattgag ttggtctgtc
     4141 tctaggggat cagatttggg cccctgagtg ccaattgagc gcggagcgcc tgctccaaga
     4201 gatgggggtc gtgcacgttg ctgtcggcgg cggccaatcg ggccggcact ccaccaggca
     4261 ccccctcccc cgaatcgagc gacgggtcga acaggaccgt gtcgggatgc aggccgtgac
     4321 ggtgcagcgc ccggacgtga tcggcgacgc cgtaccccgc cgtttcgccc tcctggggcc
     4381 gcaggttgca cacgtacacg aggtgcgccg acgaccgccc gatcgcctcg gtcacggccg
     4441 ggacggccgt cgcggcgagc acgctcgtgt agagcgagcc gggtccgagg acgatcagtt
     4501 cggcggcgtc gatccgctcg aggacatcgg gtgcaacgtg agcgtccgcc ggcgacagcg
     4561 acacgcgatc gatgccagac gtgcccatca ccgccacctg gccccggacg cggcggccgt
     4621 cgacggtcag tgccacgaga tcgaccggct cgacggtcgc tggcaacacc cggccgcgca
     4681 ctccgagcag cttgccgagc tcgtcgagcg ccgaggtgag cccaccgcac acctccgaca
     4741 tcgccgacag gaggagattg ccgagggcgt ggccgtcgag ttcgccctcg tcgaagcgga
     4801 actccatcga ccgggcgagc gccgagttcg gctcggcaag ggcgaccagg cacttgcgaa
     4861 ggtccccggg cgccgcttgc gccgtggaac ggcgcagccg gcccgtcgag ccgccgtcgt
     4921 cggcgaccga gaccaccgcc gtcacgtcgt cggtgatgcg ccgcagcgca cggagggtgg
     4981 tggcgagccc gtggccgcca ccgacggcga cgatcacttc tcgacgtccc ggtggtggat
     5041 gcgggcgtcc actccgaagg ccttgatgcg gtcggcgacc gcctccgcca tggcgaccga
     5101 gcggtgccga ccaccggtgc acccgagcgc gatcgtcagg tacgccttgc cctcctggcg
     5161 gtacgccggc agcaacatct cgaggagtga cgacaaccga tcgaggaacg gctcggtctc
     5221 gtcgagcg
//
LOCUS       DAOSOA010000237         5228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15935_length_5228_cov_4.361106, whole genome shotgun sequence.
ACCESSION   DAOSOA010000237 DAOSOA010000000
VERSION     DAOSOA010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15935_length_5228_cov_4.361106"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PKY13_12910"
     CDS             complement(<1..797)
                     /locus_tag="PKY13_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80887.1"
                     /translation="MQKRRWMFWGSVGVSAAVLATGLVAVARSEPVPAVVAVRALAPA
                     PLAVTMNVLDTGRRVAMGGRVTVSVEAIGTDVDALTLWDDDRLVATFEPESPVPGVGH
                     TFEWLGLRPGPHLLHATAHGSKGTAHSAPIGVEVFLTSTTPTELPVSAPSGTTAAMLA
                     AATGVDEGSITADGEIADATAPGSGSAAATRPAFVVDAPAVAEAAAADLADSAAEAVA
                     GVGTDVVAGSPDTGDSSDDDSAESSGSDASSAPGLTAQRDGCAVSVRV"
     gene            841..1818
                     /locus_tag="PKY13_12915"
     CDS             841..1818
                     /locus_tag="PKY13_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT80888.1"
                     /translation="MPALTVIAKAALPVLMLAGGGVAWASSGGASEPTGAAQAWVDSP
                     MDGERLEPGTVRVRAHASAPGGVTRIELAVDGRTVADESAGGDDRLGVVTLDWEATAG
                     VHELQVRGESDQGWSGYSSTVTVQVGEPAAVPTTTTTTTTTSVPATTVVDTTTSSTAD
                     TTTPTTVVAAPTTTRAGSPRTPAPRTTVPGAPATTPPTSPGGTAPPTTATPSGPATTK
                     APGTTAPATTAYPAPTVSVSGPSTQFVGIPVTLTATGAASGRPFSLVISVQSSPGGNM
                     VPITTCTSSPCSVNRTFNDVGSYEYFATITVNGVGTQSASKSILIKLIG"
     gene            complement(1808..3133)
                     /locus_tag="PKY13_12920"
     CDS             complement(1808..3133)
                     /locus_tag="PKY13_12920"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOT80889.1"
                     /translation="MTEQLYGTETQKAIDNFPISGERVPLPVIHWLARIKGAAATVNA
                     RLGKLDPSIAGAIEDAAAAVAAGRYDDQFPIDVFQTGSGTSTNMNVNEVLSALTGGSA
                     HPNDHVNMGQSSNDTFPSAVHLAALDQVTVALLPALAHLEYTLGTKARELHDVVKSGR
                     THLMDAVPVTLGQEFSGYAAQIRLGIKRVSSTRERVGQIPLGGTAVGNGLNTPPEFAG
                     EVRAILAEQSGLPWISAPEDPFEAQANRDALVELSGALKVVAVSLTKIAQDIALMSSG
                     PRAGIGEIVLPELQKGSSIMPGKVNPVIPEVVMQVAAQVIGNDTAITIGGMQGQFELN
                     VRVPLIARNLLQSIHLLTTAATAFADTCIAGIVANKRGADRSAHATLAVATALNEIIG
                     YDAATKIVKEATATGEPLRDVAIANGVDPEVYDRVVDLRAIAAGNGFSR"
     gene            3310..3900
                     /locus_tag="PKY13_12925"
     CDS             3310..3900
                     /locus_tag="PKY13_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80890.1"
                     /translation="MTHAPDAELMQRIADGDAASFEMVYRRHVDQVIRTATARCTDPH
                     EVADVVAETFLAVWRNADGFDPERGSVAQWISGIAARRFLDLRRSERRRRAVRERVVG
                     RADITVADLDELVDQIDAERAADAIGPALRRLPWRQRVVFELVAVEGMSVTAAAEALG
                     MSASAVSMRLSRARRALRVDLAVPSVASFTSPGGDT"
     gene            3897..5165
                     /locus_tag="PKY13_12930"
     CDS             3897..5165
                     /locus_tag="PKY13_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80891.1"
                     /translation="MNEHFKDQLYLAIMSEIQANPRAVPEPRRVPVTGLVAAAAVLTV
                     AAGGAVFMSIDRPHTVSVATDDTGPTTTAVDDSAYEGGFVFGVPHGRTFLVPTESLGK
                     RSSGPNYPAGTVSFRLASADPRVAPMTVSPSADHFETHAVETIAGRELRLPNGMTPPD
                     GATDADVSGPWMWNEADGTQWMAQGLLADLRAALPDLDYTDGAMVLGPSLVAAPIEDT
                     APTPERFRVRDERFEIGIADDSATTFDFVDPRRGALALVDIGGRSGIVYAGLGISWRA
                     DDHTVISLQRSTASYGGAAPTEDELLAAARSVRPASVAEMKALRVWRGAVDERVILRV
                     PEAVRSEEGVGVRLTDLDVVENGGDIGTFNAGVVPFENGFAIPLGPDRIGHRLVFRLD
                     LNDGELGCPATVTVPPQGAEPVEVELPLDC"
BASE COUNT          759 a         1832 c         1849 g          788 t
ORIGIN      
        1 acacgaacgg acacggcgca cccgtcccgc tgggcggtga ggccgggcgc ggacgaggcg
       61 tcggagcccg atgactcggc cgagtcgtcg tcggacgagt cgccggtgtc cggggatccg
      121 gcgacgacgt cggtgccgac tccggcgacc gcctcggcag cggaatcggc gaggtcggca
      181 gcggccgcct ccgcgaccgc cggtgcatcg acgacgaagg ccggtcgtgt cgcagcggcc
      241 gaccccgatc cgggcgcggt cgcgtcggcg atctcaccgt cggcggtgat cgacccctcg
      301 tcgacaccgg tcgccgctgc gagcatcgct gcggtcgtgc cgctcggcgc ggacacgggc
      361 agctccgtgg gtgtggtcga ggtcaagaac acctcgacgc cgatgggcgc cgagtgggcg
      421 gtgcccttgc tgccgtgggc ggtggcatgg aggaggtggg gcccggggcg gagacccagc
      481 cattcgaagg tgtggccaac tccgggaacg ggcgattcgg gctcgaaggt ggcgacgagc
      541 cggtcatcgt cccacagcgt gagcgcgtcg acatcggtgc cgatcgcctc gaccgagacg
      601 gtcacgcgac cacccatcgc gacacgacgg ccggtgtcga gcacgttcat cgtgacggcc
      661 agcggagcgg gcgcgagggc gcgtacagcg accactgccg gcaccggttc gctgcgggcg
      721 acggccacca gaccggtcgc caggaccgcc gcactgaccc cgacggaacc ccagaacatc
      781 cagcgccgct tctgcacgac tcgacggtac cgtgacgtcc atgcagatcc acccgggccg
      841 atgcctgcgc tgacggtcat cgccaaggca gcgcttccgg tcctcatgct cgccggcggc
      901 ggggtcgcat gggcgtcgag cggcggtgcg tcggagccga cgggcgcagc gcaggcgtgg
      961 gtcgactccc cgatggatgg cgagcggctc gaacccggca cggtccgcgt ccgcgcccac
     1021 gcgagcgcgc ccggcggcgt cacgcgaatc gagctcgccg tcgacggtcg tacggtcgcc
     1081 gacgagtccg ccggcggcga cgatcgtctc ggtgtggtca ccctcgactg ggaggcaacg
     1141 gcgggagtgc acgagctgca ggtccgcggc gagtccgacc agggttggag cgggtacagc
     1201 tcgacggtca ccgtgcaggt gggcgagccc gcggcggtgc ccaccacgac gaccacgacc
     1261 acgaccacct cggtccccgc caccaccgtg gtcgacacga cgacgtcgtc cacggcggac
     1321 acaacgaccc ccaccacggt cgtcgccgca ccgacgacga cccgagccgg atcgccgcgc
     1381 acgcccgcgc cgcgcaccac ggtcccgggc gccccggcca ctacaccacc gacgtcgccc
     1441 ggcggcactg ctcccccgac cacggcgacg ccgtcgggcc cggccaccac gaaggccccc
     1501 ggcacgaccg ctccggcgac gactgcctac ccggccccga ccgtgagcgt gtcggggccg
     1561 tcgacccagt tcgtcggcat cccggtcacc ctgacggcga ccggcgctgc gtcgggtcgg
     1621 cccttcagtc tggtgatctc ggtccagagt tcgcccggcg gcaacatggt gcccatcacg
     1681 acctgcacgt cgagcccgtg cagcgtcaac cgcaccttca acgacgtcgg cagctacgag
     1741 tacttcgcga cgatcaccgt caacggagtc gggacgcaga gcgcgtcgaa gtccatcctc
     1801 atcaagctca tcggctgaac ccgtttccgg ccgcgatggc gcgcaggtcg acgactcggt
     1861 cgtagacctc ggggtcgacc ccgttggcga tggccacgtc gcgcagcggc tcacccgttg
     1921 ccgtcgcctc cttgacgatc ttcgtcgcgg cgtcgtagcc gatgatctcg ttgagcgcgg
     1981 ttgccacggc gagcgtcgcg tgggccgacc gatcggcacc ccgtttgttc gccacgatgc
     2041 cagcgatgca ggtgtcggcg aacgcggtcg ccgccgtggt caggaggtgg atcgactgga
     2101 gcaggttgcg ggcgatgagc ggcacccgca cgttcagctc gaactggccc tgcatgccgc
     2161 cgatcgtgat ggcggtgtcg ttgccgatca cctgggcggc gacctgcatc acgacctcgg
     2221 ggatgaccgg attgaccttg cccggcatga tcgacgagcc cttctgcagc tcgggcaaca
     2281 cgatctcgcc gataccggcg cgtggtcccg acgacatcag cgcgatgtcc tgcgcgatct
     2341 tcgtgagcga gacggcgacg accttgaggg cccccgacag ctcgacgagc gcgtcgcggt
     2401 tggcctgggc ctcgaagggg tcttcggggg ctgagatcca gggcaggccc gactgctcgg
     2461 ccaggatggc gcggacctcg ccggcgaact cgggcggggt gttgaggcca ttgcccacgg
     2521 cggtgccgcc gagcgggatc tggccgaccc gttcacgggt cgagctcacc cgcttgatgc
     2581 ccaaccgaat ctgggcggcg tacccggaga actcctggcc gagggtcacg gggacggcgt
     2641 ccatcaggtg ggtgcgcccc gacttcacca cgtcgtgcag ctcgcgggcc ttggtcccga
     2701 gggtgtactc gaggtgcgcg agggccggca acaacgcgac cgtgacctgg tcgagggcgg
     2761 cgaggtgcac cgccgagggg aacgtgtcgt tcgaactctg ccccatgttg acgtggtcgt
     2821 tggggtgcgc cgacccgccg gtgagggcgg agagcacctc gttgacgttc atgttcgtcg
     2881 acgtgcccga tccggtctgg aacacgtcga tggggaactg atcgtcgtac cgaccggcgg
     2941 cgaccgccgc ggcggcgtcc tcgatcgcac cggcgatcga ggggtcgagc ttgccgagcc
     3001 gggcgttgac ggtggcagcg gcgcccttga tccgcgccag ccagtggatc accggcagcg
     3061 gcacccgttc ccccgagatc gggaagttgt cgatcgcctt ctgggtctcg gtgccgtaca
     3121 gctgctcggt catctcgtac ctccggtcgt ggtggaacct acccggcggc accgccaggg
     3181 agtcctcgtc gaaccggatc ggcccgagcc ggtgcggctc agcccgatcc ggacgacggt
     3241 gcgcccgatc cgagaaatgt tgttggatcg tcgtgtggag ggacgtagac gtgacatgag
     3301 gccccgcctg tgacccacgc tcccgacgcc gaactgatgc aacggatcgc cgacggcgac
     3361 gccgcgtcct tcgagatggt gtatcgccgt cacgtcgacc aggtgatccg caccgcgacc
     3421 gctcgctgta ccgatcccca cgaggtggcc gacgtggtcg ccgagacgtt cttggcggtc
     3481 tggcggaatg ccgacggctt cgaccctgag cggggttccg tggcgcagtg gatctccgga
     3541 atcgccgccc gccggttcct cgacctccgc cgtagcgagc gacgtcgtcg ggcggtccgt
     3601 gaacgcgtcg tcgggcgtgc cgacatcacc gttgccgacc tcgatgagct ggtcgaccag
     3661 atcgacgccg aacgtgccgc cgacgcgatc ggcccagcgc tgcggcgctt gccttggcgt
     3721 caacgtgtcg tgttcgagtt ggtcgccgtc gaggggatgt ccgtcacggc tgctgccgag
     3781 gcgttgggca tgtcggcatc agcggtgagc atgcgcctca gccgtgcccg tcgggcactc
     3841 cgcgtcgact tggcggtgcc ctccgtcgcg tcattcacat cacccggagg tgacacatga
     3901 acgaacactt caaggaccag ctgtacctcg ccatcatgag tgagatccag gcgaacccgc
     3961 gggctgtccc cgagccacga cgtgtcccgg tcacgggact cgtcgcggcg gccgccgtgc
     4021 tgaccgtcgc tgccggcgga gctgtgttca tgtcgatcga tcggccccac accgtctcgg
     4081 tcgcgaccga cgacaccggt cccacgacga cggccgtcga cgactccgcc tacgagggcg
     4141 ggttcgtgtt cggtgtcccc cacggacgca cgttcctcgt tccgaccgag tcgctcggga
     4201 agcgttcgag cgggccgaac tatccggcgg gaacggtctc gttccggctg gcatcggccg
     4261 acccgcgcgt ggcgccgatg accgtctcgc catcggctga ccacttcgag acgcacgccg
     4321 tggagacgat cgccggacgt gagctgcggt tacccaacgg gatgacacct cccgacggtg
     4381 cgaccgacgc cgacgtgagc gggccgtgga tgtggaacga ggccgacggc acgcagtgga
     4441 tggcccaggg cctgctggcc gacctccgcg cggccctgcc cgacctcgac tacaccgacg
     4501 gtgcgatggt gctcggccca tcgctcgtcg ccgcgccgat cgaggacacg gcgccgacgc
     4561 cggaacggtt ccgggttcgc gacgaacgat tcgagatcgg catcgccgac gattccgcca
     4621 cgaccttcga cttcgtcgat ccccgacggg gcgccctcgc gctcgtcgac atcggaggcc
     4681 gctccggcat cgtgtacgcc ggactcggaa tctcgtggcg ggccgatgac cacaccgtga
     4741 tctcgctcca gcggtcgacg gcctcgtacg gcggcgctgc gccgaccgag gatgagctgc
     4801 tcgctgcggc caggtccgtg cggcccgcat cggtggccga gatgaaagcg ctgcgcgtgt
     4861 ggcggggcgc ggtggacgaa cgggtgatcc tgcgggtgcc cgaggccgtc cgctccgagg
     4921 aaggggtcgg tgtgcggctc accgatctcg atgtcgtcga gaacggtggt gacatcggga
     4981 cgttcaacgc cggcgtcgtc ccgttcgaga acggcttcgc catcccgctc ggtcctgatc
     5041 ggatcggcca ccgcctggtg ttccggctcg atctgaacga cggcgagctg ggttgcccgg
     5101 cgacggtcac agtgccgcct cagggtgcgg agccggtcga ggtcgagctc ccgctcgact
     5161 gctgatccgg ctcgctcagt cgatccggac gacggtgcgc ccgatcgagc gccgctgttc
     5221 gtggtcgt
//
LOCUS       DAOSOA010000238         5219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15980_length_5219_cov_5.963013, whole genome shotgun sequence.
ACCESSION   DAOSOA010000238 DAOSOA010000000
VERSION     DAOSOA010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15980_length_5219_cov_5.963013"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..117)
                     /locus_tag="PKY13_12935"
     tRNA            complement(35..117)
                     /locus_tag="PKY13_12935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(82..84),aa:Leu,seq:caa)
     gene            145..303
                     /locus_tag="PKY13_12940"
     CDS             145..303
                     /locus_tag="PKY13_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80892.1"
                     /translation="MSSNHVSPQSAATRQRILFVVAVVAVVTAWRERAFARNRARYAA
                     TQAPQPRP"
     gene            complement(341..808)
                     /gene="bcp"
                     /locus_tag="PKY13_12945"
     CDS             complement(341..808)
                     /gene="bcp"
                     /locus_tag="PKY13_12945"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOT80893.1"
                     /translation="MADSTPTVGKKAPAIKLLDQHEAEFSLAAQKGKHVLVYFYPKAD
                     TPGCTTQACGLRDIVSEVGDTVIVGISPDKPAKLEKFDTKYELGFTLLSDVEHAVAEA
                     YGVWQLKKNYGKEYMGVVRSAFLVGPDGKVTHAWPKISPKDTPTNLLAALAEG"
     gene            complement(914..1210)
                     /locus_tag="PKY13_12950"
     CDS             complement(914..1210)
                     /locus_tag="PKY13_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT80894.1"
                     /translation="MNKTELVETIAVRTELSKKDVDAVLGAFWDIIAQTVAKGKDAVT
                     IPGWIKFERVTRDPRTVRNPQTGESMKIGKTNAVKITAGATLKAIAKGTKPAPK"
     gene            complement(1281..2078)
                     /gene="trpA"
                     /locus_tag="PKY13_12955"
     CDS             complement(1281..2078)
                     /gene="trpA"
                     /locus_tag="PKY13_12955"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOT80895.1"
                     /translation="MTAASTTYGQLETQLRQRRELGVKSLVVYLTGGLPGWLDMVRAA
                     ADAGADAIEIGIPFSDPVMDGPIIQEASDRALAAGATPPSILAEAATLDAGVPLSVMT
                     YYNLVYRFGLERFAQTAAESGVCAAILPDLPLEESGEWCAAADAAGIETVMLAAPTAP
                     PERLPRICARARGFVYGVGLLGITGERDSLALSALDIAGRLKAVTDVPVLVGVGISTP
                     QQAVETCAVADGVIVGSAVVRRALEVGTPEGVADLVAEFRAALDAKC"
     gene            complement(2075..3298)
                     /gene="trpB"
                     /locus_tag="PKY13_12960"
     CDS             complement(2075..3298)
                     /gene="trpB"
                     /locus_tag="PKY13_12960"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOT80896.1"
                     /translation="MTEIVTKTVSVGSETESVMGDPVNGRFGEFGGQFVPETLVPACQ
                     ELEASFKEAWADPAFRAELNHLLTEYAGRPSPLTECLRLSEELGCRILLKREDLNHTG
                     SHKINNVLGQALLAKRMGKTRLVAETGAGQHGVASATAAALMGMECVVYMGEVDMARQ
                     ELNVFRMRLLGAEVRPASSGSRTLKDAVNEAMRDWVATVESSHYCLGSVMGPHPYPWM
                     VREFHRVIGIETAEQFRSKVGGSPDYVVACVGGGSNAIGIFSGFADAPSKLIGVEPAG
                     GAAVGRGVPGIVHGMRSFLMQDEFGQVEEAQSISAGLDYPGVGPEHAHLADIDRAEYH
                     PVTDPEVIDAFKLLSRKEGIIPALESAHAIAWVSRERERLAGSTVVINLSGRGDKDVG
                     QMMDILGPELMGEHA"
     gene            complement(3409..4074)
                     /locus_tag="PKY13_12965"
     CDS             complement(3409..4074)
                     /locus_tag="PKY13_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOT80897.1"
                     /translation="MWVKICGITNEEDALMAVALGADAVGFVFAPSTRQVTVGTARDI
                     ARRLPPEIITIGVFRDQDPHFVSQTVLDAGLRGVQLHGHETPAMAAELSPRPQTLIVA
                     FPAGSPAIDRFDEYHADAMLLDGTTPGSGQVFDWDLVDSIRDHRRLILAGGLDPDNVG
                     AAVARVNPWGVDVSSGVEASPGQKDPRLVQAFIRAARAAEPAAYASDGGREPFDWREA
                     GDL"
     gene            complement(4085..4909)
                     /locus_tag="PKY13_12970"
     CDS             complement(4085..4909)
                     /locus_tag="PKY13_12970"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOT80898.1"
                     /translation="MATYLDRIIAFHRERAATDARSAEQLIEECAALPPTRGFTAALR
                     GADRLGVISEVKRRSPSKGDLFADLDPAALAASYEAGGASCLSVLTDGPHFGGSAADL
                     ATARAATSLPVIRKDFTVSALDVLDARLMGADCVLLIAAALSDDELAEFGALAGAIGL
                     DALVETHDEAEVERALATTSTGLIGVNQRDLVTFEVDQERAVRVAAAIPADVVRVAES
                     GVRGVDDAVALAAAGYDAVLVGEHLVTSGDPAAAIAALRVPRDPAGRVPRSSTAHR"
     gene            complement(4953..>5219)
                     /locus_tag="PKY13_12975"
     CDS             complement(4953..>5219)
                     /locus_tag="PKY13_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80899.1"
                     /translation="DDDDEWRYVDDVTDPETAPGTVVQLAPIALVVLGLVFVVGGGVL
                     RALPVMAFGGVFVALGLVGFLILPLAVMAKASTVEQRRERNRRR"
BASE COUNT          828 a         1863 c         1732 g          796 t
ORIGIN      
        1 caatgttctc ggggtgatca ctccatcaca agcgtgcccg gggtgggact cgaacccaca
       61 cgccctctcg gacagcggat tttgagtccg ccgcgtctgc cattccgcca cccgggccgc
      121 cgggagagcg tagcctgcac ctcgatgtcc tcgaaccacg tttcaccgca gtccgcggcg
      181 acgagacaac ggatcctgtt cgtcgtcgcc gtcgtggcgg tcgtgacggc atggcgcgag
      241 cgggcgttcg cccgcaaccg agcgcggtac gccgcaaccc aggctcccca gccgcgtccc
      301 tgatcccgtc gcccgatcct gtcgcctgat ctcggtcggg ctacccctcg gcgagggccg
      361 cgaggaggtt ggtcggcgtg tccttcgggc tgatcttcgg ccaggcgtgg gtcaccttgc
      421 cgtcggggcc gaccaggaac gccgagcgca cgacgcccat gtactccttg ccgtagttct
      481 tcttgagctg ccacacgccg taggcctcgg cgacggcgtg ctccacgtcg gagaggagcg
      541 tgaacccgag ctcgtacttc gtgtcgaact tctcgagctt ggccggcttg tcggggctga
      601 tgcccacgat gacggtgtcg ccgacctccg acacgatgtc gcggagccca cacgcctggg
      661 tcgtgcaacc cggcgtgtcg gccttcgggt agaagtacac gagcacgtgc ttgcccttct
      721 gggcggcgag ggagaactcg gcctcgtgct gatcgagcag cttgatcgca ggggccttct
      781 tgccaacggt gggagtcgaa tcagccatgc ccgcaaccta gtgcagccaa aaatgcgagg
      841 gaggagcagg cgagcctgct cctccctcga acttcgtgca ctcgttcagc gacgcgccgg
      901 agcgcgtctc agatcacttc ggggcaggct tggtgccctt ggcgatggcc ttgagggtcg
      961 cgccggcggt gatcttgacg gcgttggtct tgccgatctt catggactcg cccgtctggg
     1021 ggttgcggac cgtgcggggg tcacgggtga cacgctcgaa cttgatccaa cccgggatcg
     1081 tgaccgcgtc cttccccttg gcgacggtct gggcgatgat gtcccagaac gcgccgagca
     1141 ccgcgtcgac gtccttcttg gacagctcgg tgcgaacggc gatggtctcg accagttcag
     1201 tcttgttcat tgtggtgacc ctcccgggtt catgtgatga agttacaacg gtgtaactct
     1261 accgtgtttt cggggttttg tcagcatttg gcgtccaacg cggcccggaa ctcggccacg
     1321 agatcggcca ccccctcggg cgtgccgacc tcgagcgcgc gccgaacgac cgcggatccg
     1381 acgatcacac cgtcggccac ggcgcacgtc tcgaccgcct gctgtggcgt cgagatcccg
     1441 acacccacca acaccggaac gtcggtgacc gccttgagcc gaccggcgat gtcgagcgcc
     1501 gagagcgcca agctgtcgcg ctcaccggtg atacccagca acccgacgcc gtagacgaag
     1561 cctcgggcgc gggcgcagat gcgcggcaac cgttccggcg gagcggtcgg cgcggcgagc
     1621 atcacggtct cgatccccgc ggcgtcagca gcggcgcacc actcccccga ctcctcgagc
     1681 ggcaggtcgg gcaggatcgc cgcgcacacg ccggattcgg ccgccgtctg ggcgaaccgc
     1741 tccaacccga accggtagac gaggttgtag taggtcatga ccgacaacgg gacgccggcg
     1801 tcgagcgtcg ccgcctcggc gaggatgctc ggcggcgtgg caccggcggc aagcgctcgg
     1861 tccgaggcct cttggatgat cggcccgtcc atgaccgggt cggagaaggg aatgccgatc
     1921 tcgatcgcgt cggcgccggc gtccgccgct gcccgcacca tgtcgagcca acccggcagc
     1981 ccgccggtca ggtacacgac caggctcttc acacccagct cgcgacgctg acgcagctgc
     2041 gtctcgagct gcccgtacgt cgtcgaggcg gcggtcacgc gtgctctccc atcaactcgg
     2101 ggccgaggat gtccatcatc tggcccacgt ccttgtcgcc gcggcccgac aggttgatca
     2161 ccacggtcga accggccagc cgctcccgct cacgggacac ccaggcgatc gcgtgcgccg
     2221 actcgagcgc cgggatgatg ccctccttgc ggctgagcaa cttgaacgcg tcgatgacct
     2281 cggggtcggt gacggggtgg tactcggcac ggtcgatgtc ggcgaggtgc gcgtgctccg
     2341 gcccgacgcc cgggtagtcg aggcccgccg agatcgactg ggcctcctcg acctggccga
     2401 attcgtcctg catcaggaac gagcgcatac cgtgcacgat gccggggacc ccgcgcccga
     2461 ccgctgctcc gccagccggt tcgacaccga tcagcttcga cggggcatcg gcgaacccac
     2521 tgaagatccc gatggcgttg gacccgccgc cgacacaggc gacgacgtag tcgggagacc
     2581 ccccgacctt ggagcggaac tgctcggccg tttcgatgcc gatcacacgg tggaactcac
     2641 gcaccatcca cggatacggg tgcggcccca tgaccgaacc caggcagtag tgactcgact
     2701 cgacggtggc gacccagtca cgcatggcct cgttgaccgc atccttgagc gtccgactcc
     2761 ccgagctcgc cggccggacc tccgctccga ggagtcgcat gcggaacacg ttgagctcct
     2821 ggcgggccat gtcgacctcg cccatgtaca ccacacactc catgcccatc agcgccgcag
     2881 cggtggcgct cgccaccccg tgctgcccgg caccggtttc ggccaccagc cgggtcttcc
     2941 ccatccgctt ggcgagcagc gcctggccca gcacgttgtt gatcttgtgg gagccggtgt
     3001 ggttgaggtc ctcacgcttc agcaagatcc gacagcccag ctcctcggac agccgcaagc
     3061 actcggtgag cggcgacggt cgccccgcgt actcggtcag caggtggttc agctcggccc
     3121 ggaatgccgg atcggcccac gcctccttga acgacgcctc caactcctgg cacgccggaa
     3181 ccagcgtctc gggcacgaac tgcccgccga actcgccgaa ccgtccgttg acgggatcgc
     3241 ccatcaccga ctcggtctcc gaccccacac tcaccgtctt cgtcactatc tcagtcatgt
     3301 cgcccactca tctcgttctc acaggtcagc catgcgattg tgcccagacg gggccgaatc
     3361 gcgtcgaact cgtctcgttg caccgatcgg gggacggccg gcgatcgatc agagatcgcc
     3421 ggcctcgcgc cagtcgaagg gctcgcggcc gccatcggat gcgtacgcgg cgggctcggc
     3481 cgcacgcgcg gcgcgaatga acgcctgcac gagacggggg tccttctgac cgggcgacgc
     3541 ctcgactccg ctcgacacgt cgacccccca ggggttgacc ctggccacgg cggcaccgac
     3601 gttgtcgggg tcgagaccac cggcgaggat caggcggcga tggtcgcgga tcgagtcgac
     3661 caggtcccag tcgaagacct ggccgctccc cggagtcgtg ccgtcgagga gcatcgcgtc
     3721 ggcgtggtac tcgtcgaagc ggtcgatggc gggagaaccc gcggggaacg cgacgatcag
     3781 cgtctgcgga cgcggcgaga gctcggcggc cattgccggg gtctcgtgcc cgtggagctg
     3841 cacaccccgg agcccggcgt cgagcaccgt ctgggaaacg aagtgcgggt cctgatcacg
     3901 gaacacgccg atcgtgatga tctccggcgg aagccgacgg gcgatgtcac gggcagtgcc
     3961 gaccgtgacc tgtcgggtcg acggggcgaa gacgaagccg acggcatcgg caccgagggc
     4021 gaccgccatc agcgcgtcct cctcgttcgt gatgccgcag atcttcaccc acatggcgcc
     4081 aaacctagcg gtgcgccgtc gaggagcggg gtacccgtcc ggccgggtca cgaggaaccc
     4141 gcagcgcggc gatcgccgcc gccggatcgc ccgacgtcac cagatgctcg cccacgagca
     4201 cagcgtcgta gcccgccgcc gccagcgcga ccgcgtcatc gacaccgcgc acgccgctct
     4261 cggctacacg gaccacgtcg gcggggatgg ccgccgccac gcgcacggca cgctcctgat
     4321 cgacctcgaa ggtgacgagg tcgcgctggt tcaccccgat caggcccgtc gaggtcgtcg
     4381 cgagtgcccg ctcgacctcg gcctcgtcgt gggtctcgac gagggcgtcc aacccgatcg
     4441 ccccggcgag ggcaccgaac tcggccagtt cgtcgtcgct caacgccgcc gcgatcagca
     4501 gcacacagtc ggctcccatc agccgggcgt cgaggacgtc gagcgcggac accgtgaagt
     4561 ccttgcggat gaccggcaag ctcgtcgcgg cccgggctgt ggcaaggtcg gcggccgatc
     4621 caccgaagtg ggggccgtcg gtgagcaccg acaggcagga cgcaccaccg gcctcgtagc
     4681 tcgctgccag cgccgccgga tcgagatcgg cgaacaggtc tcccttcgac ggcgaccggc
     4741 gcttgacctc ggagatgacc ccgagtcggt cggcgccgcg cagcgccgcc gtgaacccac
     4801 gggtcggggg cagggcggca cactcctcga tcagctgctc cgcggagcgg gcgtcggtcg
     4861 cggcgcgttc acgatggaag gcgatgatgc gatcgaggta ggtggccatc gggggcgacg
     4921 gtagcgcgtc gcgggaccgg cgccgcacgg gtttaccggc ggcggttgcg ctcacgacgc
     4981 tgttcgacgg tgctcgcctt cgccatcacc gccaacggga ggatcaggaa cccgaccagc
     5041 ccgagcgcca cgaagacgcc gccgaacgcc atcaccggga gggcccgcag caccccacca
     5101 ccgacgacga acaccaggcc caacaccacc aacgcgatcg gtgccaactg cacgacggtg
     5161 cccggcgcgg tctcggggtc ggtcacgtcg tcgacgtacc gccactcgtc gtcgtcgtc
//
LOCUS       DAOSOA010000239         5215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15997_length_5215_cov_5.700581, whole genome shotgun sequence.
ACCESSION   DAOSOA010000239 DAOSOA010000000
VERSION     DAOSOA010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15997_length_5215_cov_5.700581"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKY13_12980"
     CDS             <1..756
                     /locus_tag="PKY13_12980"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT80900.1"
                     /translation="RSLGTSESPGTVVCTVSGAVVEPGVAEVEMGTTLAEVIDEIGGG
                     ALPEHRIVAALPGVSGAMIVADAFDVALTYEAMAEIGSGLGSAGFLVFDDHDDLVAVA
                     AGVSRFLYVESCGQCTPCKQDGGAISGALDALLDGSLGEAERSDALVTVRERLDTVAD
                     GARCALARQHEEVVGSILDAFPAAVTSYVDLPGTPRDRMLIGELVDLRAGITLLDERI
                     LRRQPDWSDNEVDSGETPVERLTDHRSNSTIVR"
     gene            830..2464
                     /locus_tag="PKY13_12985"
     CDS             830..2464
                     /locus_tag="PKY13_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT80901.1"
                     /translation="MSLSVEHLAYAHPGGTVLFDDVSFKVPAGTVSALVGGNGVGKTT
                     LMRLVAKELTPLDGEIAVSGEVHYLPQDLGIGSTDVPIRLMLTRAAPSHVRAAGEALA
                     DVEARLAAGDDAAAFEIGDALERWGALGGYQLEAMWDEVCRRVLGAGLSEVGDRLIST
                     MSGGERKRLVLDAVFETEPAVLLLDEPDNFLDIPAKEWLQERLAATKATVLLISHDRE
                     LLCASPKRIVTLEASGAWVHGESYTTYDDASFQRQERLAADRSRWEEEERRLFHFYKI
                     MKQRAASSGNSSRADAAETRWKRYVEAGPPPAPAPRHRITPNLVGADSGRIALRCDDL
                     EVDGLVLPFTAEVHFGDRVGLVGPNGTGKSHLMRTIAGELAPSGGSVTLGARINLGYF
                     SQMNRRAEFEDNELFDIARGRVTTDTAAMGALGRYGLATASDRLPSTLSGGQRARLDV
                     MCLELDGCNLLLLDEPTDNLDLESSEVLQEALEGFTGTVIAVSHDRAFLRSLSTFWHL
                     DEDGIVREFPEIDLVMPVLTGRAAADSVKHARVLTV"
     gene            complement(2469..3605)
                     /locus_tag="PKY13_12990"
     CDS             complement(2469..3605)
                     /locus_tag="PKY13_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOT80902.1"
                     /translation="MDLTVRATPFYYATMLAEHRWLKAHRDDPAYDAGQFERRDTTAN
                     MTMAHLSVVAPLLNKQFRRHLELGKGRAAVPMLATAAAAATTATVADAVARRRRSRTT
                     AELRDVATTDERDDAVARRVEQVAGAAAVAALATGGLVVTSTWAQRFAAPRLWDRRLT
                     DLGTGVAGWATALVGWDLIYYWNHRFMHESRFMWAIHVPHHSSERYNLSVALRQPVAD
                     GLGTFVPYGLLSFLGVRPSMIETARSVNLLYQYWVHTETIPKLGRFEAQMNTPSHHRV
                     HHGSNPQYLDRNHGGILISWDKAFGTFEPEDEKVVYGLTKNLNTFSPLSIIFREYRDI
                     VRDVFRAKGWRNRLSFVFRGPGWAYERHRESEPPAEVVEHQGVA"
     gene            complement(3939..4011)
                     /locus_tag="PKY13_12995"
     tRNA            complement(3939..4011)
                     /locus_tag="PKY13_12995"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3976..3978),aa:Lys,seq:ctt)
     gene            complement(4083..4156)
                     /locus_tag="PKY13_13000"
     tRNA            complement(4083..4156)
                     /locus_tag="PKY13_13000"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4120..4122),aa:His,seq:gtg)
     gene            4292..4933
                     /locus_tag="PKY13_13005"
     CDS             4292..4933
                     /locus_tag="PKY13_13005"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOT80903.1"
                     /translation="MASTITLVLTVVGSFVGGAFLWTLGEYVLHSSFHWAKGKGFASR
                     AHLDHHVHASWHFDPIITLAWLGVVLFGVAYGYLGSLIVPASVSYGVGAGWTVGYFFY
                     EWHHRASHLRGPRNRWERWVRINHFNHHFGHPMMNQQVSIPLWDIVFRTYYTPDIVKV
                     PRRLAMPWLFDDEGNLKAEYAADYEIVGDESTTNERKLALDKARAYANLEPVA"
     gene            complement(4974..>5215)
                     /locus_tag="PKY13_13010"
     CDS             complement(4974..>5215)
                     /locus_tag="PKY13_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80904.1"
                     /translation="CGMRPEHCDAHHVAQWWRDLGTTDIKLMAFLCRHHHGVIHRKGW
                     DLRIGDDGWCWITTPSGINLWCQRHGTIRSGAPPG"
BASE COUNT          800 a         1711 c         1827 g          877 t
ORIGIN      
        1 cgctcgctcg gcacctccga gtcgcccggc accgtggtct gcacggtctc gggcgcggtg
       61 gtcgagcctg gagttgccga ggtcgagatg ggcacgacgc tcgccgaggt catcgacgag
      121 atcggcggcg gagcgttgcc cgagcaccgg atcgtcgccg cactcccggg cgtgtccggc
      181 gcgatgatcg tcgccgacgc gttcgacgtg gcgctcacgt acgaggcgat ggcggagatc
      241 ggctcgggac tggggtcggc cggattcctg gtgttcgacg atcacgacga cctcgtcgcc
      301 gtcgctgccg gggtgtctcg gttcctctac gtcgagtcgt gcgggcagtg caccccgtgc
      361 aagcaggacg gcggggcgat ctccggggcg ctcgacgcac tgctcgacgg ttcgctcggc
      421 gaggccgaac gctcggacgc cctcgtcacc gtccgggaac ggctcgacac cgttgccgac
      481 ggcgcgcgct gcgcgcttgc ccgccaacac gaggaggtgg tgggcagcat cctcgacgcg
      541 ttccccgctg cggtgaccag ctacgtcgac ctccccggca cgccccgtga tcggatgctg
      601 atcggtgagc tcgtcgacct ccgcgccggc atcacccttc tcgacgagcg gatcctccgc
      661 cgtcaaccgg actggtccga caacgaggtc gattcggggg agaccccggt ggagcgactg
      721 accgaccacc gttcgaactc caccatcgtg cgctgaggga ctgtccgccg gttcgtcccg
      781 ctgatatcgg cgtcgggggc ccggtggcgg tgccgtaacc tctgccgcca tgagtctgag
      841 cgtcgagcac ctggcgtacg cccatcccgg tgggacggtg ctgttcgacg acgtgtcgtt
      901 caaggtcccg gccggcacgg tgagcgcgct ggtcggcggc aacggcgtcg gcaagacgac
      961 gctcatgcgc cttgttgcca aggagctgac gccgctcgac ggtgaaatcg ccgtgtcggg
     1021 cgaggtgcac tacctgccac aggatctcgg catcggctcg accgacgtgc cgatccgatt
     1081 gatgctgacg cgtgcggcac cgtcccacgt gcgcgccgcc ggtgaagccc tcgcggacgt
     1141 cgaggcccga ctcgccgccg gcgacgacgc ggcggctttc gagatcggcg acgcgttgga
     1201 acggtggggg gccctcggtg gctaccaact cgaggcgatg tgggacgagg tgtgtcggcg
     1261 cgtgctcggc gccggcctgt ccgaggtcgg tgaccggttg atcagcacga tgtcgggcgg
     1321 tgagcgcaag cggctcgtgc tcgacgcggt cttcgagacc gagccggcgg tgctgctcct
     1381 cgacgagccc gacaacttcc tcgacatccc cgcgaaggag tggttgcagg agcgactcgc
     1441 cgctaccaag gccacggtgc tgctcatctc ccacgaccgt gagctgctgt gcgcgagtcc
     1501 caagcggatc gtcacgctcg aggcgagcgg cgcgtgggtg cacggcgaga gctacacgac
     1561 ctacgacgac gccagcttcc agcggcagga gcgtctcgcc gccgatcggt cacggtggga
     1621 ggaagaggaa cggcggctgt tccacttcta caagatcatg aagcagcggg cggcctcgag
     1681 cggcaactcg tcgcgtgccg acgctgcgga gacccgctgg aagcggtacg tcgaggccgg
     1741 gcctccgccg gcgccggcgc cccggcatcg catcacgccg aacctggtcg gtgccgacag
     1801 tggtcgtatc gcgttgcgct gtgacgacct cgaggtcgac ggcctggtgc tgcccttcac
     1861 ggccgaggtg cacttcggcg atcgggtcgg gctcgtcggt cccaacggca ccggcaagtc
     1921 gcacctgatg cgcacgatcg ccggcgagct ggccccgtcg ggcgggtccg tcacgttggg
     1981 cgcccgtatc aacctgggct acttctccca gatgaaccgg cgagccgagt tcgaggacaa
     2041 cgagctgttc gacatcgccc gcgggcgggt caccaccgac acggcggcga tgggtgccct
     2101 cggccggtat ggcctcgcca cggcatcgga ccgactgccg tcgacgctgt caggtggtca
     2161 gcgggcacga ctcgacgtca tgtgcctcga gctcgacggg tgcaacctgc tcctgctcga
     2221 cgagcccacc gacaacctcg accttgagtc gtcggaggtg ctgcaggagg cgctcgaggg
     2281 gttcacgggc acggtgatcg cggtctcgca cgatcgcgcg ttcctgcggt cgctctcgac
     2341 gttctggcat ctcgacgagg acggcatcgt tcgggagttc cccgagatcg acctcgttat
     2401 gccggtgctg accgggcggg ccgcggccga ctcggtgaag cacgccaggg tgctgacggt
     2461 ctgacccgtc aggcgacgcc ttggtgctcg accacctcgg cgggaggctc ggactcgcgg
     2521 tgtcgttcgt aggcccagcc cggcccacgg aacacgaacg acagacggtt gcgccatccc
     2581 ttcgcccgga acacgtcgcg gacgatgtcg cggtattcgc ggaagatgat cgacagcggc
     2641 gagaacgtgt tgaggttctt ggtgaggccg tagacgacct tctcgtcctc gggctcgaag
     2701 gtgccgaacg ccttgtccca ggagatcagg atgccgccgt ggttgcggtc gaggtactgc
     2761 gggttgctcc cgtggtgcac ccggtggtgt gacggcgtgt tcatctgggc ctcgaagcga
     2821 cccagtttgg gaatcgtctc ggtgtgcacc cagtactggt agagcaggtt gaccgaacgc
     2881 gcggtctcga tcatcgacgg gcgcacgccg aggaacgaca ggaggccgta cggcacgaag
     2941 gtgccgaggc cgtcggccac cggctgacgt agggcgaccg agaggttgta ccgctcgctc
     3001 gagtggtggg gcacgtggat cgcccacatg aaccggctct cgtgcatgaa ccggtggttc
     3061 cagtagtaga tgaggtccca gccgacgagg gcggtcgccc aaccggcgac gccggtgccc
     3121 aggtcggtga ggcgccggtc ccacaaccgc ggtgcggcga agcgctgtgc ccacgtcgac
     3181 gtcacgacca ggccgccggt cgcgagcgcc gccacagctg cggctccggc cacctgctcg
     3241 acacggcgcg ccacggcgtc gtcgcgttcg tcggtcgtcg caacgtcgcg gagttcggcc
     3301 gtcgtccggc tccgtcgccg acgcgccacg gcatcggcca cggtcgcggt cgtcgccgcc
     3361 gctgccgcag tcgcgagcat cggcacagcg gcgcggccct tgccgagctc gaggtggcgg
     3421 cggaactgct tgttgagcag gggagcgacg acgctcaggt gcgccatggt catgttggcg
     3481 gtggtgtcgc gccgctcgaa ctggccggcg tcgtaggccg ggtcatcgcg gtgagccttc
     3541 agccagcggt gctccgccag catggtcgcg tagtagaacg gcgtcgcgcg gacggtcaga
     3601 tccatcggtg ccccctttcg ccgtccattg tgcccgatgg cgggccgggg gctcgcctcg
     3661 tcaggagtcg gtgcgggaga aggcgtcgcg aatcgaccag acggcggtcg cgaggaggcc
     3721 gagtgagatc gcggcgatcg gaatcatgtt gtcgatggtc tgcgccgtgg ttcccacgag
     3781 tggtcctctc ctcgaactcg acgaggaggg tacgacggct tttcgcccgt cggccatggg
     3841 caatctgggc cattcggtgc gcaccgtgcg accgatcacg ccaggaacaa gcagagcccc
     3901 cgattgctcg gaggcccggt acttcgatgt tcttcgtgtg cgccccctgg gacttgaacc
     3961 cagaacctgc ggattaagag tccgttgctc tgccaattga gctagaggcg ctcgtcgctg
     4021 tgacccggcg agtgtagtgc gacgacgacc tcgctcgaca agccgaggga cgtcgtctcc
     4081 ggggtgagtg aggggtctcg aacccccgac ctccagtacc acaaactggc gctctaacca
     4141 actgagctac acccaccaag ggcgagtcac tataacccgc gcaccccgat cgatcgaaac
     4201 gagttggtgg gcgagacggt gccggtcgac catgacgagc gtcacagaag aaaagcgaga
     4261 acatcgttct cgattcttgt atggtgacca catggcctcc accatcaccc tcgtgctcac
     4321 ggttgtcgga tcgttcgtcg ggggagcgtt cttgtggacg ctcggcgagt acgtcctgca
     4381 ctcgagcttc cattgggcga agggcaaggg gttcgcatcc cgggcgcatc tcgaccacca
     4441 cgtccatgcc agctggcatt tcgacccgat catcacgctg gcgtggctcg gggtcgtgct
     4501 gttcggcgtg gcctacgggt acctcgggtc gctgatcgtc ccggcgtcgg tgtcctatgg
     4561 cgtcggtgcc ggctggaccg tcggctactt cttctacgag tggcaccacc gtgcctcgca
     4621 cctccgtggg ccgcggaatc gctgggagcg ttgggtgcgg atcaaccact tcaaccacca
     4681 cttcgggcac ccgatgatga atcagcaggt gagcatcccc ctgtgggaca tcgtcttccg
     4741 cacctactac acgcccgaca tcgtgaaggt gccgcgtcgc ctcgccatgc cgtggctgtt
     4801 cgacgacgag ggcaacctca aggccgagta cgcggccgac tacgagatcg tcggcgacga
     4861 gtcgacgacg aacgagcgca agctcgcgct cgacaaggcg cgggcctacg cgaacctcga
     4921 accggtcgcc tgaccgacgg gtgcagcgct gacgacggcg gcaccacgcg gcgctagccg
     4981 ggtggtgcgc cggatctgat ggtgccgtgg cgttggcacc agaggttgat accggagggt
     5041 gtggtgatcc agcaccatcc gtcgtcaccg atccggaggt cccaaccctt tcgatggatg
     5101 acaccgtggt gatggcggca caggaacgcc atgagcttga tgtcggtcgt tccgaggtcg
     5161 cgccaccact gggcgacgtg atgggcgtcg cagtgttcgg gtcgcattcc gcagc
//
LOCUS       DAOSOA010000240         5215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_15999_length_5215_cov_4.771512, whole genome shotgun sequence.
ACCESSION   DAOSOA010000240 DAOSOA010000000
VERSION     DAOSOA010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15999_length_5215_cov_4.771512"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3557
                     /locus_tag="PKY13_13015"
     CDS             <1..>3557
                     /locus_tag="PKY13_13015"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOT80905.1"
                     /translation="SSISTDRRTITCNIGSRLAGSLVELPLVAYPLGTVPNGTTKTVD
                     FTVAYNDPGSSNARITQAGGSVELAVSAGKNPDLSHNDNVAPVSGIQYIADTTSAATS
                     STIAGFVTDGGTPVAGTTVALVDTNNNPVLIPGTSTPFSATTDGAGSYSFTGVPTGVV
                     RTKITAGAPAGKVPVGPMSYLNTVQGQSFTNNFDYATPAVGTGTATISGTLFHDSNLN
                     GVNNAEPAIDRVTLTLTGYGHDGTPVSRTVDTAANGSYSFSVPPSGPNGYKITASGRK
                     VVKYLAVVTNEYNVHVGDAQTKTVDIAWSTDVNRGKNFVTRFPIALKAPASGKGVASL
                     SGPITWTENVPAGATVASCGRVGQNGTQFYYLPGGTGGGLNNVVNSGTWTCTQAGGPG
                     TPVTVTVNGADTSGTTFPTRNQYSQVLPADARYLAVGYVAIAQPDPGCGVSIGTRTVS
                     SSGFSATDVGGNTMGDAYSQTDTLTAAALPSGRDCTPLPGQSGIQGYDKHFTKDESHG
                     WAAPANEDYWSTSNLHNTGGNSHVPASAPFGSQIYTYNASDVWDSTNINVCDKFDVTR
                     FGLINRIGVSPATPFAITVTNPQSPYANRLLRQYTSTAAFDAASATSGAFIVEFGVGT
                     PYASLAAQRSATCNESITWYAWDPATPVDQAVLDGAIVYRVRSKAFQPGDAIRVRAAM
                     IAKSTPGGTYAGVGLNPVPNPDPTLANTIGAYTSFGDGSSYAQPNYQTRFADPGNGIV
                     RINKYDSAASSGTTLTSAVSGNTVGQTLYVTANGVYPDSGLTMGNVTVVDTLPAGLAY
                     VAGSANPAPTSVVPGAGGTTVVTWELGNLSTDTGSATITYQAGVSPDVSTGSTLTNTA
                     VVSSTDDPSPVGLRTDSHQLAITANAEFKIFKVTPDPKVEPEDTFRYRLGYKNLSATA
                     LTSQDFIDVLPYSGDGRTTGYDGVYTLDPNTADPIVPDNASDDIYVTTAAPATITRDP
                     RAAVNTLPSTGAVTWCLVPDDLGGAGCPTDIHDPAITALRVTSTTSMSLAAPTRYIDV
                     YLRPTGDAVGNKFGNNFSATGAGLGTYVQSNAPVVDVARGTVSNLVWHDLNANGRQDG
                     GEPGISGVQVTLTGKDYAWDGDSYEIRNDYDASPLTTTTNGSGLYSFSDLNHGTFKVN
                     FPLTTSGGLSLTKQDNATDIVDSDADPATGDTAEFCLGEA"
     assembly_gap    3558..3657
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3658..>5215
                     /locus_tag="PKY13_13020"
     CDS             <3658..>5215
                     /locus_tag="PKY13_13020"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOT80906.1"
                     /translation="SPTDSSGIYGFGGLEPGPYRVQITPANGWQATRKDSGSDSSDSD
                     VDPATNRIAVNAASGVTNNTYDAGYYRPTGFGDRVWVDDDADGIQDPGEADLEGVRVD
                     LLDNSSNPVTNTLGQPIFTTTDANGYYEFTNLRSGTYKARFTKPFGYSITTQRPSGGT
                     NGAGAADDSDPDRNGVATVTLESGDPFLADVDAGMFKPVTIGDKVWADTDRDGTQDPG
                     EPGVSGVTVALFDQSGSAVLNTSGAAYTTTTDSAGRYTFTDLFPKPASNPSDYYRVRF
                     TLPAGYKWTGSGPITAPDQTSVPTTGSDTTDSDTTWTTRTQSTTFSGNLYLSQSETNE
                     TVDAGIYRPARVGNLVWRDDDADGIQDATETGQTGITVSLLQRTGTGDDGLADTSDDV
                     FDPALDDDGGAVAPVTTNSSGSYSFTNLATGVYRVRYEWTGGAHLAPTIADASGNAID
                     DVDSDAVATATTRRAETGPVLLTYGDIDNNVDAGFTPTSALGDRVWEDLDGDGTQDAG
                     EPGVDGAEVTL"
BASE COUNT          926 a         2038 c         1496 g          655 t
ORIGIN      
        1 tcgagcatct ccaccgaccg tcgcaccatc acgtgcaata tcggctcccg gctggccggc
       61 tcgctcgtcg aactccccct cgtcgcctac ccgctcggga ccgtgcccaa cgggacgacc
      121 aagaccgtcg acttcaccgt cgcctacaac gaccccgggt cctcgaacgc tcggatcacc
      181 caggcaggcg gttcggtcga gctggccgtg agcgccggca agaaccccga cctcagccac
      241 aacgacaacg tcgcgccggt ctcgggcatc cagtacatcg ccgacacgac ctccgccgcc
      301 acgtcgagca cgatcgccgg cttcgtgacc gacggtggca cccccgtggc cgggacgacc
      361 gtcgccctcg tcgataccaa caacaacccc gtgctgatcc ccggcacgtc gaccccgttc
      421 tcggccacga ccgatggagc gggttcctac tcgttcaccg gtgtgcccac cggcgtcgtc
      481 cgcacgaaga tcaccgccgg cgcgccggcc ggcaaggtgc cggtcggacc gatgagctac
      541 ctgaacaccg tccagggcca gtccttcacc aacaacttcg actacgcgac cccggcggtc
      601 ggaacgggta ccgccaccat cagcggcacc ctgttccacg acagcaacct caacggtgtg
      661 aacaacgccg agccggcgat cgaccgcgtc acgctcacgc tcaccggcta cgggcacgac
      721 ggcaccccgg tgtcgcgcac cgtcgacacc gccgccaacg gctcgtactc gttctcggta
      781 ccaccgtcgg gcccgaatgg gtacaagatc accgcttcgg gccgaaaggt cgtgaagtac
      841 ctggcagtcg tcacgaacga gtacaacgtc cacgtcggtg acgcccagac caagaccgtc
      901 gacatcgcat ggtcgaccga cgtcaaccgc ggaaagaact tcgtcacgcg cttccccatc
      961 gccctcaagg caccggcatc gggcaagggc gtggcctcgc tgtcgggacc gatcacctgg
     1021 accgagaacg tgccggccgg cgccaccgtc gcgagctgcg gccgcgtggg ccagaacggc
     1081 acgcagttct actacctgcc gggcgggacc ggcggtggcc tcaacaacgt cgtcaactcc
     1141 ggtacgtgga cctgcacgca ggccggggga cccggcacgc ccgtcaccgt gaccgtcaac
     1201 ggcgccgaca cgtcgggcac cacgttcccg acccgcaacc agtacagcca ggtccttccc
     1261 gccgacgccc gctacctcgc cgtcggctac gtggcgatcg cccagcccga ccccggctgc
     1321 ggcgtgtcga tcggcacccg caccgtgtcc tcgagcggct tctccgccac cgacgtcggg
     1381 ggcaacacga tgggcgacgc gtacagccag accgacacgc tgacggccgc agcgctcccg
     1441 agcggacgcg actgcacccc gctccccggc cagagcggca tccagggcta cgacaagcac
     1501 ttcaccaagg acgagtccca cggctgggcc gcaccggcca acgaggacta ctggtccacc
     1561 tcgaacctcc acaacacggg gggcaactcc cacgtgcccg cctcggcacc gttcggcagc
     1621 cagatctaca cctacaacgc atcggacgtg tgggattcga cgaacatcaa cgtctgcgac
     1681 aagttcgacg tgacccgctt cgggctgatc aaccgcatcg gcgtcagtcc ggcaacgccg
     1741 ttcgccatca cggtcaccaa cccccagtcg ccgtacgcca accgcctcct ccgccagtac
     1801 acgtcgaccg cggcgttcga cgcggcatcg gcgacgtccg gcgcgttcat cgtcgagttc
     1861 ggcgtcggca ccccctacgc cagcctcgcc gcccagcggt ccgccacctg caacgaatcg
     1921 atcacgtggt acgcctggga cccggcgacc ccggtcgacc aggccgtgct cgacggggcg
     1981 atcgtgtatc gcgtgcgctc caaggccttc cagcccggcg acgccatccg ggtccgggcg
     2041 gcgatgatcg ccaagtccac cccgggcggc acctacgccg gcgtcgggct gaacccggtc
     2101 ccgaaccccg atccgacgct cgccaacacc atcggcgcct acacctcgtt cggtgacggc
     2161 tccagctacg cccagcccaa ctaccagacc cgtttcgccg atcccggcaa cggcatcgtg
     2221 cgcatcaaca agtacgactc cgccgccagc tcgggcacca cgctcacgag cgccgtgagc
     2281 ggcaacaccg ttgggcagac gctgtacgtc acggcgaacg gcgtctaccc cgactccggc
     2341 ctcacgatgg gcaacgtcac ggtcgtcgac acgctgcccg ccggcctggc gtacgtcgcc
     2401 ggctcggcca acccggcgcc gacctccgtc gtccccggcg ccggcggcac caccgtcgtg
     2461 acctgggagt tgggcaacct gtccaccgac accgggtcgg cgaccatcac gtaccaggcc
     2521 ggcgtctcgc cggacgtctc gacgggctcg accctcacca acaccgcggt cgtgtcgtcc
     2581 accgatgacc cgagccccgt cggcctccgt accgacagcc atcaactcgc gatcaccgcc
     2641 aacgccgagt tcaagatctt caaggtcaca cccgacccca aggtcgaacc cgaggacacg
     2701 ttccggtacc ggctgggcta caagaacctg tcggccacgg cgctgacgag ccaggacttc
     2761 atcgacgtcc tcccctactc cggcgacggc cgcacgaccg gctacgacgg cgtgtacacc
     2821 ctggatccga acacagccga cccgatcgtc cccgacaacg cgtccgacga catctacgtg
     2881 acgaccgccg cgcccgccac gatcacccgt gatccgcgcg ccgccgtcaa caccctcccg
     2941 tcgacgggcg ccgtgacctg gtgcctcgtg cccgacgacc tcggcggcgc cggctgcccc
     3001 accgacatcc atgacccggc gatcaccgcc ctgcgcgtca cgtccacgac gtcgatgtcg
     3061 ctcgctgcgc cgacccggta catcgacgtg tacctgcgcc ccaccggcga cgccgtcggc
     3121 aacaagttcg ggaacaactt ctccgccacc ggtgccggcc tcggcaccta cgtccagtcg
     3181 aacgcccccg tggtcgacgt ggcccgcggc acggtctcga acctcgtgtg gcacgacctc
     3241 aacgccaacg gccgccagga cggcggcgaa cccggcatct ccggcgtcca ggtcaccctg
     3301 acgggcaagg actacgcgtg ggacggggac tcctacgaga tccgcaacga ctacgacgcc
     3361 agtccgctca ccaccacgac caacggcagc ggtctctaca gcttctccga cctcaaccac
     3421 ggcacgttca aggtcaactt cccgctgacg acctcgggcg gcctgtcact caccaagcag
     3481 gacaacgcga ccgacatcgt cgacagcgac gccgatcctg caaccggcga caccgccgag
     3541 ttctgcctcg gcgaggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncag
     3661 cccgaccgat tccagcggca tctacggctt cggtggcctc gagcccggcc cgtaccgggt
     3721 gcagatcacc ccggccaacg gctggcaggc gacccgtaag gactccggca gcgacagcag
     3781 cgactccgac gtcgacccgg cgaccaaccg gatcgccgtc aacgccgcct ccggcgtgac
     3841 caacaacacc tacgacgcgg gctactaccg gccgaccggc ttcggtgacc gcgtgtgggt
     3901 cgacgacgac gccgacggga tccaggaccc gggcgaggcc gacctcgaag gtgtgcgcgt
     3961 ggacctgctc gacaacagct cgaacccggt caccaacacc ctcggccagc cgatcttcac
     4021 gacgaccgac gccaacggct actacgagtt caccaacctc cgctcgggca cctacaaggc
     4081 ccgcttcacc aagccgttcg gctactcgat cacgacgcaa cgcccatctg gcggcacgaa
     4141 cggcgccgga gcggccgacg acagtgatcc cgaccgcaac ggcgtcgcca ccgtgacgct
     4201 cgagtcgggc gacccgttcc tcgccgacgt cgacgcgggc atgttcaagc ccgtcaccat
     4261 cggcgacaag gtctgggccg ataccgaccg cgacggcacc caggacccgg gcgagccggg
     4321 ggtgagcggc gtgaccgtcg cgctcttcga ccagtccggc agcgcggtcc tcaacacgag
     4381 cggcgcggcg tacacgacca cgaccgactc cgccggccgc tacacgttca ccgatctgtt
     4441 cccgaagccg gcgtccaacc cgtccgacta ctaccgggtg cggttcaccc tgccagcggg
     4501 ctacaagtgg accggctccg gcccgatcac ggcgcccgac cagacctcgg tcccgaccac
     4561 gggatccgac accaccgact ccgacacgac gtggacgacc cgaacccagt cgacgacctt
     4621 ctcgggaaac ctctacctga gccagtccga gaccaacgaa accgtcgacg ccggcatcta
     4681 ccgcccggcg cgggtcggca acctcgtgtg gcgtgatgac gacgccgacg gcatccagga
     4741 cgccaccgaa accggacaga ccggtatcac ggtgtcgctc ctccagcgaa ccggcaccgg
     4801 cgacgacggg cttgcggaca cctccgacga cgtgttcgac ccggcgctcg acgacgacgg
     4861 cggcgccgtc gccccggtca ccaccaactc gagcggctcg tactcgttca cgaacctggc
     4921 aaccggcgtc taccgggtcc ggtacgagtg gacgggtggc gcgcacctcg caccaaccat
     4981 cgccgacgcg tcgggcaacg ccatcgacga tgtcgactcc gacgccgtcg cgaccgcgac
     5041 gacgcgacgc gccgagaccg gccctgtcct cctcacctac ggcgacatcg acaacaacgt
     5101 cgatgccggg ttcaccccca catccgccct gggagacagg gtgtgggagg acctcgacgg
     5161 tgacggcacc caggacgccg gcgagcccgg cgtcgacggt gccgaggtca cgctc
//
LOCUS       DAOSOA010000241         5207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16025_length_5207_cov_7.188665, whole genome shotgun sequence.
ACCESSION   DAOSOA010000241 DAOSOA010000000
VERSION     DAOSOA010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16025_length_5207_cov_7.188665"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1521
                     /locus_tag="PKY13_13025"
     CDS             91..1521
                     /locus_tag="PKY13_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80907.1"
                     /translation="MARSRHFCAVVALTLASSLIVASCGDDAEPTRADAGTPTTRRSS
                     TSAGGSEALSSTSTSTTTTPLEDTIDLADAPPVLFVGDNDANYFEDLLEKSVVRVGPL
                     AQYQSYLISPDAKMYALASPGDAVRPSRIELISIKTGKAVSSFDVDADRVTLSEWSAD
                     STAVVARGANDWITYRTDGSSQPVEGSTGSVPGWATGDSLTSAVECSQCSRFQLIDSD
                     DHPVLVVVGLSGDGPEAGIYDPDDATIVPFEGEWATARVQAAECTRFTVLTASIDGES
                     VQALYDVDTGAIHTIEPVRGGSDCPVPSHDGRKVALAMQAGGTVVVDAATGSPVLVAR
                     QGAPLAWSADGDELLVQGNGTFVVASDGSGGKEASVKIRAFCEVGAAGKVLAEVTGAT
                     QADSGLVLYDISRNSATKIGETSLGRTCDVSKDGGWVVTGTTIVDLEEAAFTQLTRVD
                     SKGSYIRGEPIFPDRSARSPRVVAGG"
     gene            1528..3582
                     /locus_tag="PKY13_13030"
     CDS             1528..3582
                     /locus_tag="PKY13_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80908.1"
                     /translation="MRVLNRATTRPRSRPTAGAIGLALAAVVTLTLGSGEPAAAAPEP
                     CRLVDESSVSRLLGPTSRPRATSVGALDACRLTIGSLTLEVIDGTGVVGEYEAQLASH
                     GADDTQPVEDYADGAFFGWGVEGALDFVARLGQQVTIIRVFGEDPGRSFDAADPTANS
                     EDNDVSGSEVGSAMWVLMTESFVRNGGVAIPTIDDLSGLWRTTDITPCAPSRDLGVRV
                     SEFTTDGDSLRAEKVAGDACLENDAVDFEGTTSDRSGSGVSFGSTGSSSPTNGSAYRL
                     VVDSPTRVTLTGAAGALQYTLEYERLSWPGLTNTVTSSLLGIPSPSEALTVKNVALTG
                     ALSAVLLLLVVFPTTLFNSALEANLDHYRAFAARIRDRLRHRFRRPGPPTAATGAAVP
                     NSTVTTSRWRRPGGVAAYIVAAGLLFSLMQPSWGPNSATLISIIGFVGGVAIASVLSV
                     AATVVYLLFSHRNGRGYAMVEPSTLALAGFFVLASRIVGFVPGYMYGVLVNWKSAHEP
                     DDFDRGRIIAFNSSITLITAVVAWLLIPVCRDVAGSDPNVLEATPLSLIGGVFVSSVE
                     TLTIGLIPVHFLPGHTLRAHSRKAWQVLWLGGGLMFVLILLRPGLVSGASRNIVGTCA
                     LVAISSLFAIGFWAYERRRGQSASPTAGTTGTTGTTTVPGPGVPPPVPPELLPQHRPV
                     QP"
     gene            3663..4082
                     /locus_tag="PKY13_13035"
     CDS             3663..4082
                     /locus_tag="PKY13_13035"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HOT80909.1"
                     /translation="MSDTQPAPDWWQASDGKWYPPQNATLPQPTPNRTSQDMAIDQAK
                     GFLRSLFDFKLQSFVTPAVLRFLYLVAVIIIALGAGLALLVAFASKSVGLILLSIVFV
                     PVFFLLYVIFTRMYFELVAAFFQIADDIRAVRRQGDA"
     gene            4134..4796
                     /locus_tag="PKY13_13040"
     CDS             4134..4796
                     /locus_tag="PKY13_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT80910.1"
                     /translation="MQHYDVFAWVYEYGTEKLHGRHRIAAIDLLRLRPNASVLDVPTG
                     TGANLPLLIERIGPSGRVVGVDASRGMLARARKKVTSAAWDNVELVEADARVLGPDMV
                     GPTTFDAAICMLGLSVVPDWPDVFQRMFDLVRPGGRVVVMDLYLDGKRTSSVANHYYR
                     LLAQADSRRRFWEPLEAQVDDLDVVDHRWFGGVARIAAGTKPDPAVCGGHERGNAARH
                     VE"
     gene            4840..>5207
                     /locus_tag="PKY13_13045"
     CDS             4840..>5207
                     /locus_tag="PKY13_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80911.1"
                     /translation="MGTSPRDRRVFQRVETRGLPLEFDVQEPGGFLRHRRRSSRSAEV
                     IDVSVSGAQVMVATHGRWQPASMVTIRHDGSVGKALIRWEAPGPTPDTRLYGVEFLEL
                     EAPLRQYLGRPVDERRGHMP"
BASE COUNT          830 a         1892 c         1659 g          826 t
ORIGIN      
        1 acctcatctt cgacggcgac aataactcca tagtacggtg ccgcatcgat cccgcatttg
       61 agggggtcat ggctgttcga ggggagcgag atggccagat cacgtcactt ctgcgcagtc
      121 gtcgcgctga ccctggcctc gtcactgatc gtggcgagtt gcggagacga cgccgaaccc
      181 acgcgggccg acgccggcac tcccactact cgtcgctcgt ccacatcggc cggcggatcc
      241 gaggcgctgt catcgacatc gacatcaacg acgacgacac cactcgagga cacgatcgac
      301 ctcgccgacg cgccacccgt cctgttcgtc ggcgacaacg acgccaatta cttcgaagat
      361 ctgctcgaga aaagcgtcgt tcgggtgggt ccgcttgccc aataccagtc ctacctgatc
      421 agtcccgacg cgaagatgta cgccctcgca tcaccaggag acgccgttcg tccttcccgc
      481 atcgagctca tctcgatcaa aacggggaag gccgtcagct cattcgacgt cgacgccgac
      541 cgggtgaccc tgtcggaatg gtccgccgac agcaccgccg tcgtcgccag gggagcgaac
      601 gactggatca cctaccgaac cgacgggagc tcccagccgg tcgagggctc gaccggcagc
      661 gttcccggat gggccaccgg cgactcgctc acgtcagccg tcgagtgttc gcaatgctca
      721 cgattccaac tgatcgattc cgatgaccac ccggtcctgg tagtcgtcgg tctgtccggc
      781 gatggccccg aggccggcat ctacgacccc gacgacgcga cgatcgtccc gttcgagggc
      841 gaatgggcga cagctcgggt tcaagcggcg gagtgcaccc ggttcacagt gctcacggcc
      901 tccatcgacg gcgagtcggt ccaggcgttg tacgacgtcg acaccggcgc catccatacg
      961 atcgagccgg ttcgtggcgg gtcggactgc ccggtgccgt cccacgacgg ccgcaaggtc
     1021 gccctcgcga tgcaagcggg agggaccgtc gtcgtcgatg cggcgaccgg ctcgccggtg
     1081 ctggtcgccc gacagggcgc gcccctcgcc tggtctgccg atggcgacga actgctcgtg
     1141 cagggcaacg gcaccttcgt cgtcgccagt gacggctccg gcggcaagga ggccagcgtc
     1201 aagatccggg ccttctgcga ggtcggcgct gcgggcaagg tcctcgccga ggtgaccggc
     1261 gcgacccaag ccgattcggg gctggtcctc tacgacatct cccggaacag cgccacgaag
     1321 atcggcgaga ccagcctcgg cagaacctgc gacgtgagca aggacggcgg ctgggtcgtc
     1381 accggcacca ccatcgtcga tctggaggag gccgcgttca cgcaactgac ccgcgtcgac
     1441 tccaagggct cgtacatccg tggggaaccg atcttccccg accggagcgc ccggtcgccc
     1501 cgcgtggtcg ccggaggctg acagcgcatg cgtgtcctga accgcgcaac aacccgtcct
     1561 cggtcgcgac cgactgcggg cgccatcggg ctcgccctcg ctgcggtcgt caccctcacg
     1621 ctcggatccg gcgagccggc ggcagccgca cccgaaccct gccgcctcgt cgacgagagc
     1681 tcggtgagcc gcttgctcgg accgacgtcg cggccccgcg ccacgtcggt cggtgcgctc
     1741 gacgcgtgtc gtctcacgat cggttcgctc acgctggagg tcatcgacgg gaccggagtc
     1801 gtcggcgaat acgaggcaca gctggcctcc cacggagcgg acgacaccca gccggtcgag
     1861 gactacgccg acggggcctt cttcggttgg ggcgtggagg gcgcgctgga cttcgtcgcg
     1921 cgactcggac agcaggtcac catcatccgg gtcttcggcg aggaccccgg tcgatccttc
     1981 gacgcggccg accccaccgc caactccgag gacaacgacg tgagcggctc cgaagttgga
     2041 tccgcgatgt gggtcctgat gacggaatcg ttcgtccgca acggcggcgt ggccatcccg
     2101 acgatcgacg atctcagcgg gctctggcgc acgaccgaca tcaccccgtg cgctccgagc
     2161 cgagatctcg gcgtccgggt cagcgagttc accaccgacg gcgactcgct gcgggccgag
     2221 aaggtggccg gcgacgcgtg cctcgagaac gacgccgtcg acttcgaagg gaccacctcg
     2281 gaccgctccg gcagcggcgt gtccttcggg agcaccggtt cgtccagccc gaccaacgga
     2341 agcgcctacc gcctcgtcgt cgactcgccc acacgggtca ccctgacggg cgctgccgga
     2401 gcgctgcagt acaccctcga gtacgagcga ctgagttggc ccgggctgac caacaccgtg
     2461 acctcgtcgc tgctgggcat cccgtcgccc tcggaagcgc tgaccgtcaa gaacgtcgcc
     2521 ctcaccggag cgttgtcggc ggtgctgctg ctgctcgtgg tgttcccgac cacgttgttc
     2581 aacagcgccc tcgaggccaa cctcgatcac taccgggcat tcgcagcgcg gatccgcgac
     2641 cgcctgcgcc accggttccg gcggccggga ccaccgaccg cagcgaccgg cgctgcagtg
     2701 ccgaactcca ccgtcaccac ctcgcgctgg cggcgaccgg gcggagtggc ggcctacatc
     2761 gtcgccgccg ggctgctgtt cagcctcatg cagccgtcgt ggggcccgaa ctcagccacc
     2821 ctgatctcga tcatcgggtt cgtcggaggc gtcgcgatcg cgagcgtgct gtcggtcgcg
     2881 gcgaccgtgg tgtacctgct gttcagccat cgaaacggcc gcggctacgc gatggtcgaa
     2941 ccgtcgacgc tggcactggc cgggttcttc gtcctcgcct cccggatcgt cggcttcgtg
     3001 cccggctaca tgtacggggt cctcgtcaac tggaagtcgg cgcacgaacc cgacgacttc
     3061 gaccggggcc gaatcatcgc gttcaactcc tccatcacgc tgatcaccgc cgtggtggcg
     3121 tggctcttga tcccggtgtg ccgcgacgtc gccgggtccg acccgaacgt cctcgaggcc
     3181 acgccgctca gcctcatcgg cggcgtcttc gtttccagcg tcgaaacact caccatcggg
     3241 ctcatcccgg tgcacttcct tcccggccac acactgcggg cgcacagccg caaggcctgg
     3301 caggtgctct ggctcggcgg gggtctcatg ttcgtcctca tcctgctccg acccggtctc
     3361 gtgtcgggag ccagccgcaa catcgtcggc acgtgcgcgc tcgtcgcgat ctcgagcctc
     3421 ttcgccatcg ggttctgggc gtacgaacgc cgacgcggcc agtcggcgag tccgacggcg
     3481 ggcacgaccg gcacgaccgg cacgacgacc gtgccgggcc ccggggtccc gccccccgtt
     3541 cccccagagc tcctaccgca acaccgaccg gtccagccct gaccgtcggt cgccacggcc
     3601 acgttctccc tcacaccaac caaccccgcg gcacccaccg cgaacaccga ctggagcatc
     3661 agatgagcga cacccaaccg gcaccggact ggtggcaggc gtccgacggc aagtggtatc
     3721 cgccgcagaa cgccaccctc ccgcagccca ccccgaatcg cacgtcccag gacatggcga
     3781 tcgaccaggc caaggggttc ctgcgctcgc tgttcgactt caagctgcag agcttcgtga
     3841 cgccggcagt actccggttc ctctacctcg tcgccgtcat catcattgcg ctcggcgccg
     3901 gcctcgcact gctggtcgcg ttcgcgtcca agagcgtcgg gctcatcctg ttgagcatcg
     3961 tcttcgtgcc ggtcttcttc ctgctctacg tgatcttcac ccgcatgtac ttcgaactcg
     4021 tcgctgcgtt cttccagatc gccgacgaca tccgagccgt gcgacgacag ggcgacgcct
     4081 gacggaggcc cagccacccc gcgaggcatc gcgctggcta ctgtcgctcg acgatgcagc
     4141 actacgacgt gttcgcctgg gtgtacgagt acggaaccga gaagctgcac ggtcgacacc
     4201 ggatcgcggc gatcgacctc ctgcggctgc gaccgaacgc cagcgtgctc gacgtcccca
     4261 ccggaaccgg cgcgaacctc ccgctcctca tcgagcgaat cggcccgtcg gggcgcgtcg
     4321 tcggggtcga cgcgtcccgc ggcatgctgg cgcgggccag gaagaaggtc acgtctgcgg
     4381 cgtgggacaa cgtcgagctc gtcgaggccg atgcccgagt gctcggaccc gacatggtcg
     4441 ggcccacgac gttcgacgcc gcgatctgca tgctcggcct gagcgtcgtg cccgactggc
     4501 cggacgtgtt ccaacggatg ttcgacctcg ttcgccccgg cggcagggtc gtggtgatgg
     4561 acctctacct cgacggcaag cggaccagca gcgtcgcgaa ccactactac cgactccttg
     4621 cccaggccga ctcacgacgc cggttctggg aaccgctcga ggcccaggtc gacgatctgg
     4681 acgtcgtcga ccatcgctgg ttcggcggcg tggcccgcat cgccgccggc accaagccgg
     4741 accccgcggt ctgcggcggt catgagagag gaaacgcggc gagacacgtc gaatgaccta
     4801 cctctcaagt gggagtttca gcgttccgat acctcggtca tgggaacgtc cccccgcgat
     4861 cgccgcgtct tccaacgggt ggagacgcgc gggctgccac tggagttcga cgtgcaggag
     4921 ccaggtgggt tcctgcgtca ccgccgacgg tccagccgaa gcgccgaagt catcgacgtc
     4981 tcggtctcgg gcgcccaggt gatggtcgcc acgcacggtc ggtggcaacc ggcatcgatg
     5041 gtcacgatcc gccacgacgg atcggtcggg aaggccctca tccgttggga ggccccgggc
     5101 ccgaccccgg acacacggct ctacggggtc gagttcctcg agctcgaagc tccgctccgg
     5161 cagtacctcg gccgccccgt cgacgagcga cgcggccaca tgccgca
//
LOCUS       DAOSOA010000242         5199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16065_length_5199_cov_6.065513, whole genome shotgun sequence.
ACCESSION   DAOSOA010000242 DAOSOA010000000
VERSION     DAOSOA010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16065_length_5199_cov_6.065513"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKY13_13050"
     CDS             <1..469
                     /locus_tag="PKY13_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80912.1"
                     /translation="RIDLTTGQVTVVAAGLPDLNGFAFGPDGAIWAPVGGPLTALTGE
                     GYVIRIDPATGVTGALPLTFPSEPGKRGLSFPVSVKWTPDGRMLALQGIGTAALYQVD
                     PDTGVSTRFSTLGSDFGDNQVTLPDGRTFVTSFVGGMNVVAPNGTSAPFPLGA"
     gene            573..1484
                     /locus_tag="PKY13_13055"
     CDS             573..1484
                     /locus_tag="PKY13_13055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT80913.1"
                     /translation="MQIGIFDSAAGSGGSFDQLVATAAKAHEDGFASYWQPQIFGFEA
                     LSVLAVIGREVPGIGLGTGVVPTYPRHPVTMAQLALTVQAAAGDRLTLGIGLSHQMVI
                     ENMFGMSFEHPAAHMADHLAILLPLLRGDAVAHTGESLSASMALSVPKDVPAPQVVVA
                     ALGPRMLRLAGEVADGTATWMTGPKTLASHIIPSITAAAADAGRPTPRIVAALPVAVT
                     DDVAEARERCAKEFEMYGFLPSYRAMLDREGVDGPADVAIVGSADEVTSQIRALADIG
                     VTEFVAVEVGRRGAEADATRDVLRSLL"
     gene            1504..2133
                     /locus_tag="PKY13_13060"
     CDS             1504..2133
                     /locus_tag="PKY13_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT80914.1"
                     /translation="MPRSPLLDHSRAERLALVDMFASLDEAQLATQSLCAAWTVRDVL
                     AHLATPALLSNADIAKQFLRKPSFHAATVAWSERLASSSTEELIDGLRRTADDPFIPP
                     VVGPAGPLTDAVIHGEDARHPLGLRRDVPEPHLREILDFGLSRRAQPVFVPFRRLRGV
                     RFVATDLDWSGGQGAVVEGPALQVALAMFGRIGAAPGLTGDGVAVLAAR"
     gene            complement(2130..2999)
                     /locus_tag="PKY13_13065"
     CDS             complement(2130..2999)
                     /locus_tag="PKY13_13065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOT80915.1"
                     /translation="MRVSVATAYLPPDELAPIAKAADELGYHALALADHVLNLEELTT
                     EYPYTEDGSRRWEPFTPWVDPMVAIGALGAVTERLRFFTNIYVLPMRNPFPVAKAVAT
                     ASAFTGGRVSLGIGMGWCEEEFDLLDAQFRKRGARADEMIEVLAKLWSGEWVEHHGQF
                     YDFPRLEMTPVPAGPVPILVGGLSDAALRRAARNDGWISDLISTDQAAEYRLSLDRYR
                     EELGRTGDFSMIVSLNDAITVDQFRRAEDVGVTDLLTMPWAYYGGFRLPLQEKLDGMK
                     RFADDVMGPLNAA"
     gene            complement(2996..3760)
                     /locus_tag="PKY13_13070"
     CDS             complement(2996..3760)
                     /locus_tag="PKY13_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT80916.1"
                     /translation="MSLNRLSDRVVIVTGAASGIGQATAIRLASEGAKVLASDINEAG
                     LAETLATITGAGGEAITHRADISQEADAAGTVAVAVDAWGRLDGLANIAGMLRSGRTH
                     EFSLDSWNQVIAVNLTGTFLMCRAALPHLIEAKGAIVNAASTSSFFGHPWMGAYAASK
                     GAILSFTQTLAVEYATVPVRANCVAPGSVHSGITSNVSFPDDLTKDEAKLIRRIMSPT
                     GFGDPGDVGSVVALLLSDDGKHITGECIRIDGGTHA"
     gene            complement(3824..>5199)
                     /locus_tag="PKY13_13075"
     CDS             complement(3824..>5199)
                     /locus_tag="PKY13_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT80917.1"
                     /translation="LKVESRMVFDGGAYASTSHAVLFNAITHAQGPYKCDNAVVDGWA
                     VRTNNPPCGAMRGFGVVQACFAHESQMDKLAAACGIDPIELRLLNAMETGDKLITGQV
                     VDSVAPVARCINETVALPMPPEATDADDAMWLPGGAGRTADRSNIVRGVGYGVSIKNL
                     MYSEGFDDYSTARCRLEDGRAVLKFATAEVGQGFVTLAAQIARTNLGVDDVVLEPIDT
                     SVGSSGSTSASRQTWMSGGAVDLACRTVRDKLFEQVAPQLGADPLSLVIDGYDIVDLD
                     GGGRIPVAEASAGVVIDETVVYRHRPTVAPDAMGQGPSHVAFAFVAHRAVVDVDPELG
                     LVRVVQIATAQDVGVALNPLSVVGQIEGGIAQGLGLAVMEEILVTDGLIRNPSFTDYL
                     LPTFLDAPEVAISLVEEYEPQAPMGAKGVGEPPCISVTPAIVAAIRDATGRDLTRVPV
                     RPQDICL"
BASE COUNT          756 a         1854 c         1798 g          791 t
ORIGIN      
        1 tcgcatcgac ctcaccactg gccaggtcac ggtggtggcc gccgggctcc ccgacctcaa
       61 cggcttcgca ttcggtcccg acggtgcgat ctgggcgccg gtcggcggcc ccctgacggc
      121 actcaccggc gagggctacg tgatccggat cgaccccgcc accggggtca ccggcgcgtt
      181 gccgctgacg ttcccgtcgg aacccggcaa gcgcggactc tcgttccccg tatcggtgaa
      241 gtggacaccc gacggcagga tgctggcgct gcagggcatc ggcaccgctg ccctctacca
      301 ggtcgatccg gacaccggcg tgtcgacgcg gttctcgacg ctcggctccg acttcggcga
      361 caaccaggtc accctgcccg acggtcgcac cttcgtcacc tcgttcgtcg gcggtatgaa
      421 cgtcgtcgcc ccgaacggca cgagcgcccc gttcccgctc ggcgcctgac gtcctctcac
      481 gacccgctcg gtttcggtcg cggtttctcc cgctgagcgg gcgtttccgc gaccgaaagc
      541 cggcgaggtc ccggaggggt aggttgcggc gcatgcagat cggtatcttc gactccgccg
      601 ccggtagcgg tggctccttc gaccaactcg tcgccacggc ggccaaggcc cacgaggacg
      661 gtttcgcctc gtactggcag ccgcagatct tcggattcga ggcgctgtcg gtgctcgcgg
      721 tcatcgggcg cgaggtgccc ggtatcgggc tcggcaccgg tgtggtgccg acctaccccc
      781 gtcatcccgt gaccatggcg cagctcgcgc tcaccgtgca ggcggctgcc ggcgaccggc
      841 tcacgctcgg catcggcctc agccaccaga tggtcatcga gaacatgttc ggcatgagct
      901 tcgagcaccc ggccgcgcac atggccgacc acctcgcgat cctcctaccg ctgctccgcg
      961 gcgacgccgt ggcccacacc ggcgagtcgc tgtcggcgtc gatggcgctg tcggtcccca
     1021 aggacgtgcc ggccccgcag gtcgtcgtgg cggccctcgg cccgcgcatg ctgcggcttg
     1081 ccggcgaggt cgccgacggc accgccacct ggatgaccgg cccgaagacc ctcgccagcc
     1141 acatcatccc ctcgatcacc gccgctgccg ccgacgccgg ccgtccgacg ccccgcatcg
     1201 tcgcagcgct gccggtggcc gtgaccgacg acgtcgccga ggcgcgcgag cgctgcgcga
     1261 aggagttcga gatgtacgga ttcctcccga gctaccgggc gatgctcgac cgcgagggcg
     1321 tcgacggccc agccgacgtc gcgatcgtgg gctctgcgga tgaggtcacg tcgcagatcc
     1381 gggcgctcgc cgacatcggc gtcaccgagt tcgtcgcggt cgaggtcggc cgccgcgggg
     1441 ccgaagccga cgccacccgc gacgtcctgc gttccctcct gtagctccga gacgggaggt
     1501 cacatgcccc gctcgcccct cctcgatcac agccgcgccg agcgcctggc gctcgtcgac
     1561 atgttcgcgt cactcgacga ggcgcagttg gccacgcaga gcctgtgcgc cgcgtggacc
     1621 gtgcgcgacg tgctcgccca cctcgccacg ccggcgctcc tctcgaacgc cgacatcgcc
     1681 aagcagttcc ttcggaagcc gagcttccac gctgcgacgg tcgcgtggtc cgagcgcctc
     1741 gcgagctcct cgaccgagga gctgatcgac ggcctgcgcc gcacggcgga cgatccgttc
     1801 atcccgcccg tcgtcggccc ggccggtccg ctgaccgacg cggtcatcca tggcgaggac
     1861 gcccgccacc ccctcgggct ccgtcgagac gtccccgagc cgcacctccg cgagatcctc
     1921 gacttcggcc tgagccggag ggcacagccg gtgttcgtgc cgttccggcg actgcgcggc
     1981 gttcggttcg tcgccactga tctcgactgg tccggcgggc agggcgccgt ggtcgagggc
     2041 cccgccctcc aggtcgccct cgcgatgttc ggacgaatcg gcgccgcgcc gggcctcacc
     2101 ggggacggag tcgcggtgct cgcggcgcgc taggccgcgt tgagcggccc catcacgtcg
     2161 tcggcgaacc gtttcattcc gtcgagcttc tcctgcaacg ggagccggaa cccgccgtag
     2221 taggcccagg gcatcgtgag cagatcggtg accccgacgt cctcggcgcg ccggaactgg
     2281 tcgacggtga tcgcgtcgtt gagcgaaacg atcatcgaga aatcgccggt ccggccgagc
     2341 tcctcccggt agcggtcgag gctgaggcgg tactcggcgg cctggtcggt gctgatgagg
     2401 tcggagatcc aaccgtcgtt gcgagcggcc cgccgaagcg cggcatcgga cagaccaccg
     2461 acgaggatcg ggacgggacc ggcgggcacc ggcgtcatct cgagacgggg gaagtcgtag
     2521 aactgcccgt gatgctcgac ccactcgccc gaccagagct tggcgagcac ttcgatcatc
     2581 tcgtcggcgc gggcgccgcg cttgcggaac tgagcgtcga ggaggtcgaa ctcctcctcg
     2641 caccacccca tgccgatacc gagcgacacg cgaccaccgg tgaacgcgct cgccgtcgcg
     2701 acggccttcg ccaccgggaa cggattgcgc atgggcagca cgtagatgtt ggtgaagaag
     2761 cggagccgct cggtcaccgc tcccagcgcg ccgatggcga ccatcggatc gacccacggc
     2821 gtgaacggct cccagcgccg gctcccgtcc tcggtatagg ggtactcggt ggtgagttcc
     2881 tcgaggttga gcacgtggtc ggcgagcgcg agcgcgtgat agccgagctc gtcggccgcc
     2941 ttggcgatcg gcgcgagctc gtcgggcggg aggtacgccg tggcgacgct gaccctcatg
     3001 cgtgggtccc gccgtcgatg cggatgcact cgccggtgat gtgcttgccg tcgtcggaca
     3061 gcagcagggc gaccacggat ccgacatcgc ccgggtcacc gaagccggtg ggcgacatga
     3121 tgcggcggat cagtttggcc tcgtccttgg tgaggtcgtc ggggaagctg acgttggacg
     3181 tgatgccgct gtgcacactg ccgggagcga cgcagttggc ccgcaccggc acggtggcgt
     3241 actccacggc gagggtctgg gtgaacgaga ggatggcgcc cttcgatgct gcgtaggcac
     3301 ccatccacgg gtggccgaag aagctcgacg tcgacgctgc gttcacgatc gccccctttg
     3361 cctcgatgag gtgcgggagc gctgcccgac acatcaggaa ggtgccggtg aggttgacgg
     3421 cgatgacctg gttccagctg tcgaggctga actcgtgggt gcgccccgat cgcagcatgc
     3481 cggcgatgtt cgcgaggccg tcgagacgac cccaggcatc gaccgcgacg gcaaccgtgc
     3541 cggcagcgtc ggcctcctgg ctgatgtcgg cccggtgggt gatggcctcg ccgccagcgc
     3601 ccgtgatggt cgccagcgtc tcggcgagcc cggcctcgtt gatgtccgac gcgaggactt
     3661 tcgctccctc gctcgcgagg cggatggcgg tggcctggcc gatgcccgag gcggccccgg
     3721 tcacgatgac aacacgatct gacagacggt tcagcgacat cggcgcagac tagaacacgt
     3781 tccagtttcg gcgcccgtcg aacgggccac gaccggccgg ctgctacaga cagatgtcct
     3841 gcggccggac cggcacgcgg gtgaggtcgc gaccggtggc gtcgcggatg gcggcgacga
     3901 tcgccggggt caccgagatg cacggcggct cgccgacgcc cttcgctccc atcggcgcct
     3961 gcggctcgta ctcctcgacg agcgagatgg ccacctcggg cgcgtcgagg aaggtcggaa
     4021 gcaggtagtc ggtgaacgac gggttccgga tcagcccgtc ggtgacgagg atctcctcca
     4081 tcaccgcgag gccgagcccc tgggcgatgc cgccctcgat ctggccgacg accgacaagg
     4141 ggttgagggc gacgccgacg tcctgggccg tcgcgatctg cacgacgcgg acgaggccga
     4201 gctcagggtc gacatcgacg acggcgcggt gggcgacgaa ggcgaaggcc acgtggctgg
     4261 ggccctggcc catcgcgtcg ggagcgaccg tcggccgatg gcggtacacc acggtctcgt
     4321 cgatcacgac gccggcgctc gcctcggcga ccgggatccg cccgccaccg tcgaggtcga
     4381 cgatgtcgta gccgtcgatc acgagtgaca gcggatcggc gccgagctgg ggggccacct
     4441 gctcgaacag cttgtcgcgc accgtccggc acgccaggtc gaccgcgccg cccgacatcc
     4501 aggtctggcg cgacgccgac gtggagcccg acgaaccgac ggaggtgtcg atgggctcga
     4561 gcaccacgtc gtcgacaccg aggttggtgc gggcgatctg ggccgccagg gtcacgaacc
     4621 cctggccgac ctcggccgtc gcgaacttca ggaccgcccg accgtcctcg agccgacagc
     4681 gagccgtcga gtagtcgtcg aacccttcgg agtacatgag gttcttgatc gacaccccgt
     4741 agccgacgcc gcgcacgatg ttcgagcgat cggcggtccg accggcgcct ccgggcagcc
     4801 acatcgcatc gtcggcgtcg gtggcctcgg gtggcatcgg gagcgccacc gtctcgttga
     4861 tgcagcgagc gaccggcgcc acgctgtcga ccacctggcc cgtgatcagc ttgtcaccgg
     4921 tctccatggc gttgagcagg cgcaactcga tcgggtcgat cccacacgcg gccgccagct
     4981 tgtccatctg cgactcgtgg gcgaaacagg cctgcacgac gccgaacccg cgcatcgcac
     5041 cacacggcgg attgttggtg cgcaccgccc agccgtcgac gacggcgttg tcgcacttgt
     5101 acggcccctg cgcgtgggtg atggcgttga acagcaccgc gtggctcgtc gaggcgtagg
     5161 cgccaccgtc gaacaccatg cgactctcga ccttgagga
//
LOCUS       DAOSOA010000243         5171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16185_length_5171_cov_6.740422, whole genome shotgun sequence.
ACCESSION   DAOSOA010000243 DAOSOA010000000
VERSION     DAOSOA010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16185_length_5171_cov_6.740422"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PKY13_13080"
     CDS             <1..871
                     /locus_tag="PKY13_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80918.1"
                     /translation="WWEAYWYEPMDRTRMRYFARVIYATVLVTVWFIDEWAVGHATAP
                     RLFWQPMWIARVLSIPAPTPGTMTLLRVVLTVAAVVAIVLARPDTSAVARHAARAANA
                     VVFAGFSLWVIWAFSWSKVDHDKLTIVVALFTLVVVPGVGAGADRMVGWALRTVQVVF
                     VLAYPLSAISKIDKSGWLWANQATFARAIIRRGTSLGSHLAQQGELLRVSQWGFIVFE
                     VVSIVALSRNPKIRRVILPGFFFLHFFTWLAIGIHFLPHTICLVAFLPLERLRLPGWR
                     TRRVAADEQALVA"
     gene            878..>2631
                     /locus_tag="PKY13_13085"
     CDS             878..>2631
                     /locus_tag="PKY13_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 2"
                     /protein_id="HOT80919.1"
                     /translation="MSERMLWSPLRLGPVTLKNRIVFSAHLTNYATQDGHPSDQHVAY
                     YEERAAGGAGLIITEEHSTHPTDWPYEKLIHGFNPAVIDGYRRITEAVHRHGTPIFAQ
                     INHNGGQASSMFSRLPVWAPSAVPDPLFREVPKAVSTGEIAEIVESYAQVARHCREGE
                     FDGIELQCSHSSIVRGFLSPATNKRTDDYGGSLDNRARILVEIVEAVRAAIGPGLALG
                     VRICGDELIEGGTTIDDALGIARIVEATGQVDYINTSIGVATASLFMIEASMHIPPNY
                     AMFIPSAIRKVVDLPVVGVGRFKDPIQAERALQEGHCDLVGVVRGQIADADFAKKARA
                     GYTDDTRLCLSCNQECVGRMGLNRWLGCIENPRTGRESEGVGSVRLTRAPRSVLIVGA
                     GPAGLQAAIAAARNGHRVTVLERATEAGGQVRLAASVPNRAEFGDLVRNQVSECRRLG
                     VAVEYGVEADAAEIRRRNPDRVVIATGARAARPWWAPGDAEWIVDVRDVLEGRAQPTG
                     SVIVLDEIGFHHATSVAELLADRGCSVEVITPGMVVGQDLGITLDMENWWIRAGAKGI
                     VQSTELVPMGVTDAEGTHT"
     assembly_gap    2632..2731
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2732..3281)
                     /locus_tag="PKY13_13090"
     CDS             complement(<2732..3281)
                     /locus_tag="PKY13_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT80920.1"
                     /translation="MPNTGTAAAEETVVDLREPAPPATLQNDALRPRLSMVYGGGGAV
                     GIAWHVAVIDALRDAGLPVQDAPSIGTSAGSWACGAARLGLGFEEFSRFGELALPDRR
                     PGLLAGMAREIFGSTRTNDVRVSVTALPTMRRHLLDARRHDLADLIAASSAVPGVFAP
                     HQIAGTQYVDGGVRSMASADTAA"
     gene            3360..4061
                     /locus_tag="PKY13_13095"
     CDS             3360..4061
                     /locus_tag="PKY13_13095"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOT80921.1"
                     /translation="MDVSPEQFFGDLMSAFAVHADPVRAVQEQAYLKSEREFLGVTVP
                     VARKVVAAAVKQHAVDDRVDVVAVTEVCWEHPSFDARRAAVEVLSLRHAVLLPEDLDL
                     IESMLRDGETWAIVDSLAAKVVGSIVRRFPGETGPVLDRWAADPDSFWIRRSAMLALL
                     EDLRSGGGDWDRFCRYADSMLHEKEFFIRKAIGWILRDTSRKRPDLVWDWVEPRLDDM
                     SGVTKREALKYLGGR"
     gene            complement(4074..>5171)
                     /locus_tag="PKY13_13100"
     CDS             complement(4074..>5171)
                     /locus_tag="PKY13_13100"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOT80922.1"
                     /translation="ALDTAGTNPNACTRSAVTVTCPVTGPLAPGAHVDVRIPVKMLLT
                     QPTSGPIDPATAAVANRTVADPVSANDSVVASPVIDLSGDSDGDGVSDQDEMDPDGTG
                     RPVDSDGDGIPDYLDKPGVVVTGDVFRDLNRNGVHDAGEPDISGVLVKLVGPGPDGTL
                     GTGDDVTSQTTRTASPYRFTNVSNGVFDIVVDRSTLPAGVYATDDTDGGADSRVRVEV
                     AGAAVVAKDFGANYAVVSGVFTDDDGAPLANATISFTDAAGRTFTTTTDAEGNFRFEG
                     SAEAPLVLGTATLRGRTADGKEVVRTIEVARAVTVADLSRTAAGPTPAPSGTAPGELA
                     FTGGGTRSSTSAALCIIAGVVLVLVARRRRA"
BASE COUNT          712 a         1734 c         1815 g          810 t
ORIGIN      
        1 ctggtgggag gcgtactggt acgagccgat ggatcgcacc cggatgcggt acttcgcccg
       61 cgtgatctac gcgaccgtcc tggtcacggt ctggttcatc gacgagtggg cggtcggcca
      121 cgccacggcg ccgcgcctgt tctggcagcc gatgtggatc gcccgcgtgc tctccatccc
      181 ggcgccgacg ccgggcacca tgacgctgct ccgggtggtc ctgacggttg cggccgtcgt
      241 ggcgatcgtt ctcgctcgcc ccgacacgag cgcggtcgcc cggcatgcgg cgcgtgccgc
      301 caacgcggtc gtgttcgccg gattctccct ctgggtgatc tgggcgttct cgtggtcgaa
      361 ggtcgaccac gacaagctca ccatcgtcgt cgccctgttc accctcgtcg ttgtccccgg
      421 ggtcggggcc ggcgccgacc ggatggtggg gtgggcgctg cggaccgtgc aggtcgtgtt
      481 cgtgctcgcg tacccgctgt cggccatctc gaagatcgac aagtcggggt ggctgtgggc
      541 gaaccaggcg acgttcgccc gggcgatcat ccggcgtggc accagcctgg gctcgcacct
      601 cgcccagcag ggggagttgc tgcgggtcag ccagtggggc ttcatcgtct tcgaggtcgt
      661 gtcgatcgtg gccctgagcc ggaacccgaa gatccgtcgc gtgatcctcc cggggttctt
      721 cttcctccac ttcttcacct ggctcgcgat cggcatccac ttcctgccgc acacgatctg
      781 cctcgtggcg tttctccctc tggaacgact gcgcctcccg gggtggcgga cgcgtcgagt
      841 cgcagcggac gagcaagcgc tcgtagcctg accatcgatg tcggaacgca tgctgtggtc
      901 acccctgcgt ctggggccgg tgacgttgaa gaaccggatc gtgttctcgg cgcacctcac
      961 caactacgcg acccaggacg gccatccctc cgaccagcac gtggcgtact acgaggaacg
     1021 cgccgccggc ggcgccgggc tcatcatcac cgaggaacac tcgacccacc cgaccgactg
     1081 gccgtacgag aagctgatcc acggcttcaa cccggcggtc atcgacgggt accggcgcat
     1141 caccgaggca gtccaccgtc acggcacacc gatcttcgcc cagatcaacc acaacggcgg
     1201 ccaggcctcg tcgatgttca gccgcctgcc ggtgtgggcc ccgtcggccg ttcccgaccc
     1261 gctgttccgt gaggtgccca aagccgtcag cacaggcgag atcgccgaga tcgtcgagag
     1321 ctatgcccag gtcgcccgcc attgtcgtga gggcgagttc gacggcatcg agctgcagtg
     1381 ctcgcacagc tccatcgtcc gtgggttcct ctcgcccgcg accaacaagc gcaccgacga
     1441 ctacggcggc tcgctcgaca accgggcgcg catcctcgtc gagatcgtcg aagcggttcg
     1501 cgccgcgatc ggcccggggc tcgcactcgg cgtgcgcatc tgcggcgacg agctgatcga
     1561 gggcggcacc acgatcgacg atgcgctcgg catcgcccgc atcgtggagg cgaccggaca
     1621 ggtcgactac atcaacacct cgatcggcgt cgcgacggcg tcgctgttca tgatcgaggc
     1681 gtcgatgcac atcccgccga actacgccat gttcatcccg tccgcgatcc gcaaggtggt
     1741 cgacctgccg gtcgtgggcg tcggccggtt caaggacccg atccaggccg agcgagccct
     1801 gcaggagggc cattgcgacc tggtcggtgt cgtccggggt cagatcgccg acgccgactt
     1861 cgccaagaag gcgcgggccg gatacaccga cgacacccgt ctctgcctgt cgtgcaacca
     1921 ggaatgcgtg gggcgtatgg gcctcaaccg ctggctcggc tgcatcgaga atccgcgcac
     1981 gggccgtgag tccgaggggg tcggatcggt gcgcctcacg cgggcgccgc gttcggtgtt
     2041 gatcgtgggc gctgggcccg ccgggttgca ggcggcgatc gccgctgcac gcaacggtca
     2101 ccgggtgacc gtgctcgaac gggcgaccga ggccggtggc caggtgcgcc tggcggcttc
     2161 ggtgcccaac cgggccgagt tcggcgacct cgtccgcaac caggtctcgg agtgccgacg
     2221 actgggggtc gcggtcgagt acggcgtcga ggccgacgcc gccgagatcc gtcgtcgcaa
     2281 ccctgaccgc gtggtcattg cgaccggtgc ccgtgccgcc cgaccgtggt gggcgccggg
     2341 cgacgccgag tggatcgtcg acgtgcgtga cgtgctcgag ggtcgtgccc aaccgaccgg
     2401 ttcggtcatc gtcctcgacg agatcggctt ccatcacgcc acgtcggtgg ccgagttgct
     2461 cgccgaccgg ggttgttcgg tcgaggtgat cactcccggc atggtcgtcg gccaggatct
     2521 cggcatcacg ctcgacatgg agaactggtg gatccgcgcc ggtgccaagg gcatcgtgca
     2581 gtcgaccgag ttggtgccca tgggcgtgac cgacgccgag ggaacccaca cnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggcggccg tgtcggcgga cgccatcgag
     2761 cgaacaccgc cgtcgacgta ctgcgtaccg gcgatctggt ggggggcgaa caccccgggc
     2821 acagcggacg atgccgcgat cagatcggca aggtcgtggc ggcgggcgtc gagcaggtgg
     2881 cggcgcatcg tcggcaaggc ggtgaccgac acccgcacgt cgttcgtgcg ggtcgatccg
     2941 aagatctccc gagccatccc ggccaggagg ccggggcgac ggtcggggag ggcgagctcg
     3001 ccgaagcgtg agaactcctc gaaccccagc ccgagtcgag cggccccgca cgcccaggat
     3061 ccggcgctgg tgccgatgga cggggcgtcc tgcacgggca ggccggcgtc gcggagggcg
     3121 tcgatcacgg cgacgtgcca cgcaatgccg acggccccgc cgccgccgta gaccatcgag
     3181 aggcggggcc ggagggcgtc gttctgcagc gttgcgggtg gtgccggttc gcgcaggtcg
     3241 acgacggtct cctctgcggc ggcggtcccg gtgttcggca cgggggacat cgtggccgat
     3301 ttcggaggga tttcctagag gaagttcgcg gccgatcgtc ggcgtgcgac gatgtgccga
     3361 tggacgtctc gcccgagcag ttcttcggcg acctcatgtc ggcgttcgcc gtccacgccg
     3421 acccggttcg tgccgtgcag gagcaggcgt acctcaagtc cgaacgcgag ttcctggggg
     3481 tgacggtgcc cgtcgctcgg aaggtcgtcg ccgctgcggt gaagcagcac gccgtggacg
     3541 accgtgtcga tgtggtcgcc gtcaccgagg tctgctggga gcatccgtcg ttcgacgcgc
     3601 ggcgcgccgc ggtcgaggtg ctgtcgctcc gtcacgccgt actccttccc gaggacctgg
     3661 acctgatcga gtcgatgctc cgagacggcg agacctgggc gatcgtcgac tcgctcgccg
     3721 ccaaggtcgt cggctcgatc gtccgccggt tccccggcga gacggggccg gtgctcgacc
     3781 ggtgggccgc cgaccccgac tccttctgga tccgacggtc ggcgatgctc gccctgctcg
     3841 aggatctgcg ctccggcggc ggcgactggg accgcttctg ccggtacgcc gactcgatgc
     3901 tgcacgagaa ggagttcttc atccgcaagg ccatcggatg gatcctgcgc gacacatccc
     3961 ggaagcggcc cgatctcgtg tgggactggg tcgagccccg cctcgacgac atgtcgggcg
     4021 tcaccaaacg cgaagcgctc aagtacctcg gcggccgctg actccggccc ggctcaggcc
     4081 cgccgtcggc gggcgacgag cacgaggacg actccggcga tgatgcacag cgccgccgag
     4141 gtcgaggaac gggtcccgcc accggtgaag gccagctcac ccggcgccgt gccggacggc
     4201 gccggcgtcg gccccgccgc agtgcgcgac aggtccgcca cggtcacggc acgcgcgacc
     4261 tcgatcgtgc gcaccacctc cttgccgtcg gccgtcctcc cacgcagggt ggcagtgccg
     4321 agcacgagtg gcgcctccgc cgagccctcg aaccggaagt tcccctccgc atcggtggtc
     4381 gtcgtgaagg tgcggccggc ggcgtcggtg aacgagatcg tggcgttcgc gagcggcgcg
     4441 ccgtcgtcgt cggtgaacac accggagacc accgcgtagt tggcaccgaa gtccttggcc
     4501 acgacggcag ctccggccac ctcgacgcgc acccgtgagt cggcgccgcc atcggtgtcg
     4561 tcggtggcgt acacgccggc cggaagcgtc gagcggtcga cgacgatgtc gaacacaccg
     4621 ttggagacgt tcgtgaagcg gtaggggctc gcggtgcgtg tcgtctgcga tgtcacgtcg
     4681 tcgcccgtgc ccagcgtgcc gtcagggccg ggtccgacga gcttgacgag caccccggag
     4741 atgtcgggct cgccggcgtc gtggacaccg ttgcggttga ggtcgcggaa cacatcgccg
     4801 gtgacgacga cgcccggctt gtcgaggtag tcggggatcc cgtcgccgtc ggagtcgacg
     4861 gggcgtccgg tgccgtcggg gtccatctcg tcctggtcgc tcaccccgtc gccgtcgctg
     4921 tcaccgctca ggtcgatcac cggcgacgcg acgacgctgt cgttggccga caccgggtcg
     4981 gcgaccgtgc ggttggcgac ggccgcggtg gcggggtcga tgggtcccga cgtcggctgt
     5041 gtgagcagca tcttcacggg aatgcggacg tcgacgtggg cgccgggagc gagcggcccg
     5101 gtcaccgggc acgtgacggt gaccgccgaa cgcgtgcagg cgttggggtt ggtgccggca
     5161 gtgtcgaggg c
//
LOCUS       DAOSOA010000244         5153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16261_length_5153_cov_6.283052, whole genome shotgun sequence.
ACCESSION   DAOSOA010000244 DAOSOA010000000
VERSION     DAOSOA010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16261_length_5153_cov_6.283052"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKY13_13105"
     CDS             <1..567
                     /locus_tag="PKY13_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOT80923.1"
                     /translation="GFNASTFTSRVITSTGADVGAAIVGALGALSGPLHGGAPSRALD
                     LLDAIGTPDRASDWIRDAVVRGERIMGFGHAVYKTDDPRSTLLREVAQRLGGPRAEFA
                     TQVERLIVDTLAEVKPGRQLYANVEFYAGVVMEAAGLPPELFTPTFAVSRVIGWCANV
                     MEQAADNRIIRPSARYVGPVPPQPVPAP"
     gene            complement(598..1365)
                     /locus_tag="PKY13_13110"
     CDS             complement(598..1365)
                     /locus_tag="PKY13_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT80924.1"
                     /translation="MSPPRLHADEVDLDDRIMRHLVDTRFPHWADRPLRRLDTTGTDN
                     AIFRLGDDLGIRAPRLERAVQQIHRECRWIAPLAPHLPAVVHEPVAIGDPDPTYPFPF
                     LVFRWIDGTDRATRAAIAALADEPGPPVDIDRALAVWDGALAAGEWNRPPVWVHGDLL
                     PGNILVADGHLVGVVDWSSGGLGDPAADTMLAWALPSAARAAYRDALGVDDATWRRGR
                     GWVIEQAASFIAYYRDTIPDGVALARARLDAVLAADD"
     gene            complement(1397..2527)
                     /locus_tag="PKY13_13115"
     CDS             complement(1397..2527)
                     /locus_tag="PKY13_13115"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOT80925.1"
                     /translation="MPTPGLSARTRSGLLLATAVVVLTALAVVDPAGVELISAGAEPV
                     ATTSTTSSTSTTTSSTSTSSTSTVPSTTSTTTVPDPTTSTDATAGLRLSDDLLGAIAG
                     DFPDASLAQDGQVYLAYATNIETDAALLNVPVRASFDLSAWSEAIDALPVLPSWAKRG
                     STWAPTVQRIGDVWVMYFTARDVDSGLQCIGVATSATSAGPFVSSGDEPVACQTDLGG
                     SIDPTIVVDSSGGTWLVWKSDGNCCGLTTSIWSAPIGAFGSITGTPVRLLAATKRWQH
                     GVIENPSLVETDTGWVLLYSAGRWDDDTYATGFALCTDLASACTNASSTPLFDDVAGT
                     YGGGGLSVTALDDGRLVAIWHAWSTNDPDAGRSAWLAELELV"
     gene            2687..4666
                     /locus_tag="PKY13_13120"
     CDS             2687..4666
                     /locus_tag="PKY13_13120"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT80926.1"
                     /translation="MSIESRFAGTPFTDDDATIAAYLEDLSVPTLMASMVHVTGDPSW
                     IRGPIKPVGLFINEVQGFIDPADQATVRAQALAALIEFRDGGCVLPPPPGPELVKEMM
                     DFLVVETVPDEYLPMLLEELELDGVDPRVEPEHVDIGGRAPGGFGVIVIGAGMSGLLA
                     GIRLAEAGVEFTIIEKNAGVGGTWFENRYPGCRVDVGNHFYSYSFAPDDAWTEFFAQQ
                     PELQAYFERCLHEYGIADRVRFSTEVVGATWDEAAARWTVEVRPAGGDERDTEHLTAT
                     ALISAVGQLNRPKLPDIEGRDSFAGLAMHSAQWVDGTDLTGKRVAVIGSGASALQLVP
                     AIAPDVEHLTVFQRSASWMFPNPHYHDKVGPGVTWAVRHLPFYGRWYRFLLFWPACDG
                     GLAAMRIDPEYPHQDRAISEINDATREVFGQYMADQITDDPELAAKVVPDYVCLGKRT
                     LQDNGSWLAALQRPNVELVTDPIVRIRPEGVVTGGEHDVEHDVDVIVYATGFHANRYL
                     WPMEIRGRGGVVLSEQWGDEPTALLGVTVPEFPNLFCLYGPATNLAHGGSIIFMIECQ
                     VRYVMGALAALADAGEGAALAVRREAHDEYNERLQAEIDTMIWGHPSIRHSWYRSPSG
                     RIYILSPWRMVDYWDWTRRPDLAHYEPLVPAPTEV"
     gene            4746..>5153
                     /locus_tag="PKY13_13125"
     CDS             4746..>5153
                     /locus_tag="PKY13_13125"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT80927.1"
                     /translation="MPRPSPQTDRVVAVIELLSSGDDGATMTEIARHLSINPATCVHV
                     MAALTSAGVVVREPDRRYHLGPALALPGRLAERRYPLLVAARSEMVALSERFGAACFA
                     FAPDGERARLAQVTWPRDETIDRSQVRVGESVPM"
BASE COUNT          764 a         1724 c         1888 g          777 t
ORIGIN      
        1 gggttcaacg cttcgacctt cacgtcgcgg gtgatcacgt cgacaggcgc cgatgtcggt
       61 gccgcgatcg tgggtgcact cggcgcgttg tcgggaccgt tgcacggcgg ggcgccgagc
      121 cgggcgctcg atctcctcga cgccatcggc actcccgacc gggcgtccga ctggattcgt
      181 gatgccgtgg tccgcggcga gcggatcatg gggttcgggc acgccgtgta caagaccgac
      241 gacccccggt cgacgctcct gcgggaggtc gcgcaacgac tcggcggccc ccgcgccgaa
      301 ttcgccacgc aggtcgagcg actgatcgtg gacacgctcg cggaggtgaa accgggtcgg
      361 cagctgtacg cgaacgtcga gttctacgcc ggtgtggtga tggaggcggc cggcctgccg
      421 ccggaactgt tcacgccgac gttcgcggtg agccgggtca tcggttggtg cgccaacgtc
      481 atggagcagg ccgccgacaa ccggatcatc cggccgagcg cccgctacgt cggccccgtc
      541 ccaccccagc ccgtccccgc tccctgaccg gggcgggcgg cagtcacgct cccgtcctca
      601 gtcgtcggcg gcgaggacgg cgtcgagtcg ggctcgggcc agggcgacac cgtcgggaat
      661 cgtgtcgcgg tagtacgcga tgaagctcgc cgcctgctcg atcacccagc cccggccccg
      721 gcgccaggtc gcgtcgtcga cgcccagggc gtcgcggtag gcggcgcggg cggcagacgg
      781 cagggcccac gccagcatcg tgtccgcggc tgggtcgccg agtccgccgg agctccagtc
      841 gacgacgccg acgaggtggc cgtcggcgac caggatgttg ccgggcagga ggtcgccgtg
      901 cacccagacg ggcggccggt tccactctcc ggccgccagc gcgccgtccc agacggccag
      961 cgctcggtcg atgtcgacgg gcgggccggg ctcatcggcc agcgctgcga tcgcggcgcg
     1021 ggtggctcgg tcggtgccgt cgatccaccg gaagaccagg aacgggaacg gataggtcgg
     1081 atccggatcg ccgatcgcta ccggctcgtg caccaccgcc ggcaggtgcg gtgcaagggg
     1141 agcgatccat cggcactcgc gatggatctg ttggacggct cgttcaagtc gcggtgcccg
     1201 gatgcccaag tcgtcgccca ggcggaagat ggcgttgtcg gtgccggtcg tgtcgaggcg
     1261 gcggagggga cggtccgccc agtgggggaa ccgggtgtcg acgagatggc gcatgatgcg
     1321 gtcgtcgagg tcgacctcgt cggcgtggag tcggggcggc gacaccccgc cactgcagtc
     1381 ggtgcccggc ccggggtcaa acgagttcca gctcggcgag ccaggcggag cgtccggcgt
     1441 cgggatcgtt ggtcgaccag gcgtgccaga tggcgacgag gcgaccgtcg tcgagcgccg
     1501 tgaccgagag cccgccgccg ccgtaggtgc cggccacgtc atcgaagagc ggcgtcgatg
     1561 atgcgttggt gcacgccgac gcgagatccg tgcagagcgc gaagccggtt gcgtaggtgt
     1621 cgtcgtccca cctcccggcg gagtacagca gcacccaacc ggtgtcggtc tcgacgaggg
     1681 acgggttctc gatcacgccg tgctgccaac gcttcgtggc ggccaacagg cgaacagggg
     1741 taccggtgat cgatccgaat gcgccgatcg gtgccgacca gatcgacgtg gtcagaccgc
     1801 agcagttgcc gtcgctcttc cacaccagcc aggtgccgcc cgagctgtcg acgacgatcg
     1861 tcgggtcgat ggacccgccg aggtcggtct ggcacgcgac cggttcgtcg cccgacgaca
     1921 cgaacgggcc ggccgaggtg gccgaggtcg ccacgccgat gcactgcagc ccgctgtcga
     1981 cgtctcgcgc cgtgaagtac atcacccaca cgtcaccgat tcgttggacc gtcggtgccc
     2041 acgtcgaccc gcgcttcgcc cacgagggca ggacggggag cgcgtcgatc gcctcggacc
     2101 acgcgctcag gtcgaacgac gcccggacgg gcacgttgag gagtgcagcg tcggtctcga
     2161 tgttggtcgc gtaggcgagg tacacctggc cgtcctgcgc gagggaggcg tcggggaagt
     2221 cgccggcgat ggccccgagc aggtcgtcgg agagccggag gccggcggtc gcgtcggtgg
     2281 aggtggtcgg gtcggggacc gtggtggtcg aggtcgtgct ggggaccgtg gacgtcgacg
     2341 acgtggacgt gctggaggtg gtcgtcgacg tgctggaggt ggtcgaggtg gtggcgacgg
     2401 gctcggcacc ggcggagatg agctcgacgc cggccgggtc gacgacggcc agcgcggtga
     2461 ggacgaccac ggcagtggca aggaggagcc cggaccgggt acgggcgctg aggccgggcg
     2521 tcggcatccg tccacgatcg gcgtcgttgc tgggacgctg cgcagctgaa cctcagaatt
     2581 gggtgggacg tcgcggcgct atggtgcgcc ggaacacggg ccgatccggg gggatccatg
     2641 agcactgacc agccaacacc gcgagtggtc tcgtgagcgg tgtgtcatga gtatcgagag
     2701 ccggtttgcc ggcaccccgt tcaccgacga cgacgcaacc atcgccgcct acctcgagga
     2761 cctcagcgtc ccgaccctca tggcgtcgat ggtgcacgtc accggtgatc cgtcgtggat
     2821 ccgcgggccg atcaaaccgg tcggactgtt catcaacgag gtgcaggggt tcatcgaccc
     2881 ggccgaccag gcgacggtgc gagcccaggc gctcgccgca ctcatcgagt tccgcgacgg
     2941 cggctgtgtg ctgccgcctc cgcccgggcc cgagctcgtc aaggagatga tggacttcct
     3001 cgtcgtcgag acggtgcccg acgagtacct gccgatgctg ttggaggagc tcgagctcga
     3061 cggtgtcgat ccccgcgtcg agcccgaaca cgtcgacatc ggcggtcgtg cgcccggcgg
     3121 tttcggagtg atcgtgatcg gggccggtat gtcgggcctg ctcgccggca tccgtctcgc
     3181 cgaggccggc gtcgagttca cgatcatcga gaagaacgcc ggggtcggtg gcacctggtt
     3241 cgagaaccgc tatcccgggt gccgcgtcga cgtcggcaac cacttctact cgtactcgtt
     3301 cgcacccgac gacgcctgga ccgagttctt cgcccagcag cccgagctgc aggcgtactt
     3361 cgagcggtgc ctccacgagt acggcatcgc cgatcgggtg aggttctcga ccgaggtcgt
     3421 gggtgcgacg tgggacgagg ccgcggcccg ctggaccgtc gaggtgcgcc ccgcaggtgg
     3481 cgacgagcgt gacaccgagc acctgacggc gacggcgctc atcagcgcgg tgggccagct
     3541 caaccggccg aagctccccg atatcgaggg tcgcgattct ttcgccggac tcgccatgca
     3601 ctccgcgcag tgggtcgacg gcaccgacct gaccggcaag cgggtcgcgg tcatcggcag
     3661 cggtgccagc gccttgcagc tcgtgcccgc catcgccccc gacgtcgaac acctcaccgt
     3721 gttccagcga tcggcgtcgt ggatgttccc caacccgcac taccacgaca aggtcggtcc
     3781 aggggtcacg tgggcggtgc ggcacctccc gttctacggg cggtggtacc gcttcctgct
     3841 gttctggccg gcgtgcgacg gcggtctggc cgcgatgcgc atcgaccccg agtatcccca
     3901 ccaggaccgg gcgatcagcg agatcaacga cgccacccgc gaggtgttcg ggcagtacat
     3961 ggccgaccag atcaccgacg atcccgagct cgcggccaag gtcgtgcccg actacgtgtg
     4021 cctcggcaaa cgcacgctgc aggacaacgg cagttggctc gccgcgttgc agcggccgaa
     4081 cgtcgaactc gtcaccgatc ccatcgtccg catccgtccc gagggggtgg tgacaggagg
     4141 cgaacacgac gtcgaacacg acgtcgacgt catcgtctac gccaccggct tccacgcgaa
     4201 ccggtacctg tggccgatgg agatccgtgg ccgtgggggt gtggtgttgt cggagcagtg
     4261 gggcgacgag ccgacggcgc tgctcggtgt cacggtgccc gagttcccca acctgttctg
     4321 cctgtacggg ccggccacga acctcgccca cggcggctcg atcatcttca tgatcgagtg
     4381 ccaggtccgc tacgtcatgg gagcgctcgc agcgctggcc gatgccggcg agggcgcagc
     4441 gttggccgtt cgccgggagg cgcacgacga gtacaacgag cgactccaag cggagatcga
     4501 cacgatgatc tggggccatc ccagcatccg ccacagctgg taccgcagcc cgagcggacg
     4561 catctacatc ctgtcgccgt ggcgcatggt cgactactgg gactggacgc gtcgccccga
     4621 tctcgcgcac tacgaaccgc tcgtccccgc tccgacggag gtttgaaccg cggcgcacgg
     4681 gacacgtgcg ccgcggttca aacctgggac gggggcgggc ggacgtcgcc tggcacactg
     4741 tcgtgatgcc ccgcccgtcg ccgcagaccg accgggtcgt cgccgtgatc gagctgctgt
     4801 cctcgggcga cgacggtgcg acgatgaccg agatcgcccg tcacctgtcg atcaacccgg
     4861 ccacgtgtgt gcacgtgatg gcggcattga cgtcggcggg ggtggtggtg cgcgagcccg
     4921 accgccgcta ccacctgggg ccggcgctgg cgctgcccgg tcggctcgcc gaacgccggt
     4981 acccgctgct cgtcgcggcg cggtcggaga tggtggcgct gtcggagcgc ttcggcgccg
     5041 cctgcttcgc cttcgctccc gatggtgagc gggcacggtt ggcgcaggtg acgtggcccc
     5101 gggacgagac gatcgaccgc agccaggtcc gagtggggga gtcggtgccg atg
//
LOCUS       DAOSOA010000245         5086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16580_length_5086_cov_5.548201, whole genome shotgun sequence.
ACCESSION   DAOSOA010000245 DAOSOA010000000
VERSION     DAOSOA010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16580_length_5086_cov_5.548201"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PKY13_13130"
     CDS             <1..317
                     /locus_tag="PKY13_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT80928.1"
                     /translation="FLLSFYGSTPAYRPVLDAHGWGDLQTELNAMTKSNQWDRMPALI
                     DEEMLRTLAIVGSPTEVADQLVARFDGVASRVGFYFPYAIADDLIAELVSSVRERVSR
                     ST"
     gene            complement(301..861)
                     /locus_tag="PKY13_13135"
     CDS             complement(301..861)
                     /locus_tag="PKY13_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOT80929.1"
                     /translation="MTFDADELGRLIRARRTTKLLDPDTPVDPTVIEELCALATWAPN
                     HKRTEPWRFAVFTGDGRRVLGETIADALQATGGHEAKILKTRTKYLRSAAVVMVGSTP
                     GPDEMTTGENRDAVAAGISNFLLGATARGLATLWSTVSPPRSPAVAELCGFPEGTFVV
                     GAVYLSHPTGEEPLGRRSVPMITWID"
     gene            930..>3454
                     /locus_tag="PKY13_13140"
     CDS             930..>3454
                     /locus_tag="PKY13_13140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT80930.1"
                     /translation="MEPDRAPAESSPRPGTDAALAALRGTELLIESLPGAIVVWDVDG
                     TVLAWNANAERLYGWTSGNVLGHHIGELLILPAELDAAYEIALGVEAGETWTGDLTIV
                     RADATPVRIHTALRPLRNERGEIVGSIGVADDVTDQRAIEDRAAELTNHLLMALAGGE
                     LGTWRRDLDTDEIVWDENMERLCGLEPGTYDGTAESWYAMIHPDDRDATRSTIAAAVA
                     TGSSYQVEYRVLHPDGSIRWLQGRAMVTQHSDGSVAATIGCSGDVTDRKLVELENERR
                     ALEAERLLEAGRLQRRRLEFLSGLNDAAMAADDHQALMQAVTEAAVPTLGDWCSLHFF
                     EGSTVAANPDIIFAHHDPERIEWLRDLLAHHPYDPDAPLGAAAAVREGVTQFIPSFSG
                     EFRERLIDNAVRATPDQARAILDELMPTSLIAVPLVTKRGVIGAMQFVSAESGRVYDA
                     DDVALAETAAGRVAEALDAAWLRDQQRNVANVLQSALLPARLPEIDGAGIAVRYWAAG
                     AASEVGGDFYDVFAIGPDRWAVVIGDVCGTGPEAAAVTALARHTIRAAATHGASPVEV
                     LEWLNDAILASDTDRFCTVLYSTLERIDAESGHWRFTSVAGGHPLPFLARAGEAASPL
                     GTAGTLLGVMPTVRLTPSVVDLGPGDTVVLNTDGITDVPAPHGIDEATMADIVTAATA
                     VAGPAETVVEHLGSAVQRVLPIPERDDDIALVVVRIAGGDLVDLAGEDDGSVEELVAE
                     REFEPEPQSVSAAREFVIASAARPPLDDLLRLAVSELATNAVIHARSSFRVRVVAAGA
                     GVRIEVADRSERMPVRNHQPYDAVSGRGIGIVEHLSTSWGVTPT"
     assembly_gap    3455..3554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3575..5071)
                     /locus_tag="PKY13_13145"
     CDS             complement(3575..5071)
                     /locus_tag="PKY13_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT80931.1"
                     /translation="MSIEPLTDLTAGMAILAGGDQVIRVTPELADAFAPGDRLAVVGE
                     TVLHIPAAEGATAAAAVGAASDAFARMGAVTDEQITAFFEAFARRLEDTESFAPIAAA
                     NQADIEAAKARGRSTTRLVLSDTMRADMVSGLRSWATAESGRGAVIDAVEHDGWSVEL
                     RRAGLGVVGFVFEGRPNVFADACGVIRSGNTVVFRIGSDALGTARAIVEHALDPALAE
                     AGLPAGAASLVDSAAHAAGWAMFSDPRLGLAVARGSGPAVTQLGTVARQAGVPVSLHG
                     TGGAWIVADSAADGTTLAAAVLHSLDRKVCNTLNVCCIVADRADELVPVVLASLGAAA
                     DRRGTNPKLHVAAGSESYVPAEWFEPTDITRAQGAVREAGAELIALEELGTEWEWEES
                     PEISLVIVADVDEAIALFNDHSPRFVASLISTDAAAQDHFYELVDAPFVGNGFTRWVD
                     GQYALGKPELGLSNWQFGRLFGRGGVLSGDSVFTVRTRVTQTDPSVHR"
BASE COUNT          736 a         1719 c         1799 g          732 t
ORIGIN      
        1 gcttcctgct cagcttctac ggctcgacgc ccgcctatcg accggtgctc gacgcgcacg
       61 ggtggggcga tctccagacc gagctgaacg ccatgaccaa gtccaaccag tgggaccgca
      121 tgccggcgct gatcgacgag gagatgctgc gcacactcgc gatcgtcggc agtccgaccg
      181 aggttgccga ccagctcgtg gctcgattcg acggcgtcgc cagccgggtc ggcttctact
      241 tcccctatgc catcgccgac gacctgatcg ccgagctggt gagttcggtg cgggaacggg
      301 tcagtcgatc cacgtgatca tcggcaccga acgccgtccg agcggttcct cgccggtcgg
      361 gtggctgagg tagaccgcgc ccacgacgaa ggttccttcg gggaacccgc acagttcggc
      421 gacggcgggc gaccgtggcg gcgacaccgt cgaccacaac gttgccaacc cgcgggcggt
      481 cgccccgagg aggaagttgc tgatgccggc agcaacggcg tcgcggttct cgccggtcgt
      541 catctcgtcg ggcccgggtg tcgagcccac catcacgacc gccgccgacc ggaggtactt
      601 cgtgcgggtc ttgaggatct tcgcctcgtg gccgccggtg gcctgcagcg catcggcgat
      661 ggtctcgccc aggacccggc gaccgtcgcc ggtaaacacg gcgaagcgcc atggctcggt
      721 gcgcttgtgg ttcggcgccc acgtcgcgag cgcgcagagc tcttcgatga cggtcggatc
      781 gaccggggtg tcggggtcga gcagcttggt tgtccgacgc gccctgatga ggcggccgag
      841 ctcgtcggcg tcgaaagtca tggcgccatc ctcccagacc cgatcggaca ccgaggtggc
      901 ggcggtcgat ccgacctacg ctggtcaccg tggagcctga ccgagcgccc gccgagtcgt
      961 cgccccggcc cggcaccgac gcggcgctgg ccgcgctccg gggcaccgag ctcctgatcg
     1021 agagcctgcc aggggcgatc gtcgtgtggg atgtcgacgg gaccgtgctg gcctggaatg
     1081 cgaacgccga gcgactgtac ggctggacat cgggcaacgt gctcggccat cacatcggcg
     1141 agctgctgat cctgccggcc gagctcgacg ccgcgtacga gatcgcgttg ggggtcgagg
     1201 ccggtgagac ctggacgggc gacctgacga tcgtgcgcgc cgacgcgacg ccggtgcgga
     1261 tccacaccgc gctccgaccg ttgcgcaacg agcgcggcga gatcgtcggc tcgatcggcg
     1321 tcgccgacga cgtgaccgat caacgggcga tcgaggaccg cgccgccgag ctcacgaacc
     1381 atctgctgat ggcgctcgcc ggcggcgagt tgggcacgtg gcgccgggat ctcgacaccg
     1441 acgagatcgt gtgggacgag aacatggagc ggctctgcgg actggagccc ggaacctacg
     1501 acggcaccgc cgagagttgg tacgccatga tccaccccga cgaccgggac gcgacccggt
     1561 ccaccatcgc ggccgcggtg gcgacggggt cgtcgtacca ggtcgaatac cgggtgctgc
     1621 atcccgacgg ttcgatccgg tggctgcaag gtcgggcgat ggtgacccag cacagcgacg
     1681 gctcggtggc ggccacgatc gggtgctctg gtgatgtgac cgaccgcaag ctcgtcgagc
     1741 tcgagaacga gcgtcgggcg ctcgaggccg agcgactcct cgaggcgggg agattgcagc
     1801 ggcggcgact cgagttcctg tcgggcctca acgacgcggc gatggccgcc gacgatcatc
     1861 aggcgctcat gcaggcggtg acggaggctg ccgtcccgac cctgggcgac tggtgctcgc
     1921 tccacttctt cgagggatcg accgtcgcgg cgaaccccga catcatcttc gcccaccacg
     1981 atcccgagcg gatcgagtgg ctacgtgacc tgcttgccca ccatccgtac gaccccgacg
     2041 cgcccctcgg cgctgccgcc gcggtccgcg agggcgtcac ccagttcatc ccgtcgttct
     2101 ccggcgagtt ccgcgaacgg ctgatcgaca acgcggttcg ggcgacgccc gaccaggcgc
     2161 gggccatcct cgacgagctg atgccgacga gcctgatcgc cgttcccctc gtgaccaagc
     2221 gtggggtcat cggcgccatg cagttcgtga gcgccgagtc cggacgtgtc tacgacgccg
     2281 acgatgtcgc gctcgccgag acggccgccg gtcgggtcgc ggaggccctc gatgccgcgt
     2341 ggctgcgcga ccaacagcgc aacgtcgcca acgtcctcca gtcggcgctg ttgccggccc
     2401 gcctgcccga gatcgacggt gccggcatcg ccgttcgcta ctgggcggcc ggcgcggcga
     2461 gcgaggtcgg cggcgacttc tacgacgtgt tcgcgatcgg acccgatcgc tgggccgttg
     2521 tcatcggcga tgtgtgcggc accggccccg aggcagccgc ggtcaccgcg ctcgcgcggc
     2581 acacgatccg cgccgccgcc acccacgggg cgagtcccgt ggaggtgctc gagtggctca
     2641 acgacgccat cctcgcgagc gacaccgacc gtttctgcac ggtgctctac tcgaccctcg
     2701 aacggatcga cgccgagtcc gggcactgga ggttcacgtc ggtcgccggt gggcacccgc
     2761 tcccgttcct ggctcgcgcc ggcgaagcgg cgtcaccgct cggcaccgcc gggacgctgt
     2821 tgggtgtcat gccgaccgtc cgcctcacgc ccagcgtggt cgatctcgga ccgggcgaca
     2881 ccgtggtgct gaacaccgac ggcatcaccg atgtgcccgc cccgcacggg atcgacgagg
     2941 cgacgatggc cgacatcgtg accgcggcca cggcggtggc cggccctgcc gagaccgttg
     3001 tcgagcacct cgggtcggcg gtccagcgag tgttgccgat cccggaacgc gacgacgaca
     3061 tcgccctcgt cgtcgttcgc atcgccggtg gcgacctcgt cgacctggca ggcgaggacg
     3121 atggctccgt ggaggagctg gttgccgagc gtgagttcga gccggaaccg cagtcggtct
     3181 cggcagcccg ggagttcgtg atcgcgagcg cggcgaggcc tcccctcgat gacctgttac
     3241 gactcgcggt ctcggaactc gcgaccaacg cggtgatcca cgcccggagt tcgtttcgcg
     3301 tccgggtcgt tgccgccggt gctggcgtcc gcatcgaggt cgccgaccgc agcgagcgga
     3361 tgcccgttcg caaccatcag ccctacgacg ccgtgagcgg acgtgggatc ggcatcgtcg
     3421 agcacttgtc gacgagctgg ggcgtcacgc cgacnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnacgtct cggctcgatg ttgatcagcg gtgcactgag gggtcggtct
     3601 gcgtgacgcg ggtgcgcacg gtgaacaccg agtcgcccga gaggacaccg ccgcgcccga
     3661 acaggcgccc gaactgccag ttgctgaggc ccaactcggg cttgccgagc gcgtactgac
     3721 cgtcgaccca gcgggtgaac ccgttgccga cgaacggtgc gtcgaccagt tcgtagaagt
     3781 ggtcctgcgc ggccgcgtcg gtcgagatca gcgacgcgac gaagcggggg gagtggtcgt
     3841 tgaacagggc gatggcctcg tcgacgtcgg cgacgatcac caggctgatc tccggcgatt
     3901 cctcccactc ccattcggtc ccgagctcct cgagagcgat gagctcagcg ccggcctcgc
     3961 gcacagcacc ttgggcgcgg gtgatgtcgg tcggctcgaa ccactcggcg gggacgtacg
     4021 actcgctgcc cgcggcgacg tgcagcttgg ggttggtgcc acgccggtcg gccgctgcgc
     4081 cgagcgacgc cagcaccacc ggcacgagct cgtcggcgcg gtcggcgacg atgcagcaga
     4141 cgttgagcgt gttgcacacc ttgcggtcga gcgagtggag aaccgctgcg gccagggtcg
     4201 tgccgtcggc agccgagtcg gcgacgatcc aggctccgcc ggtgccgtgc aggctcaccg
     4261 gcacaccggc ctggcgggcc accgtgccga gctgcgtgac cgcaggaccc gacccgcgag
     4321 cgacagcaag gccgagccga gggtcggaga acatcgccca gccggcagcg tgtgccgccg
     4381 agtcgacgag cgacgcggca ccggcgggga gcccggcctc ggcgagggcc gggtcgagcg
     4441 cgtgctcgac gatcgctcgg gcggtgccga gagcgtcgga cccgatgcgg aacacgacgg
     4501 tgtttccgga acggatgacg ccgcacgcat cggcgaacac gttgggacgg ccttcgaaca
     4561 cgaacccgac gacgccgagg ccggcgcggc gcagttcgac cgaccagccg tcgtgctcga
     4621 ccgcgtcgat gacggcgccg cggcccgact cggcggtcgc ccacgagcgg agcccgctga
     4681 ccatgtcggc gcgcatcgtg tcgctgagga cgaggcgggt ggtggagcgg ccccgagcct
     4741 tggcggcctc gatgtcggcc tggttcgccg cggcgatcgg ggcgaacgac tcggtgtctt
     4801 ccagacggcg ggcgaacgct tcgaagaacg cggtgatctg ctcgtcggtg actgcgccca
     4861 tccgggcgaa cgcgtcgctc gctgcgccca cggcggctgc ggcggttgcg ccttcggcag
     4921 cggggatgtg gaggacggtc tcgcccacga cggcgaggcg atcgcccggt gcgaacgcgt
     4981 cggcgagttc gggcgtgacg cggatgacct ggtcgcctcc ggcgaggatg gccatgccgg
     5041 cggtcaggtc ggtgagcggt tcgatcgaca tggcgcagac gctacc
//
LOCUS       DAOSOA010000246         5082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16600_length_5082_cov_5.422717, whole genome shotgun sequence.
ACCESSION   DAOSOA010000246 DAOSOA010000000
VERSION     DAOSOA010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16600_length_5082_cov_5.422717"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..973)
                     /locus_tag="PKY13_13150"
     CDS             complement(23..973)
                     /locus_tag="PKY13_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOT80932.1"
                     /translation="MPTNALSVVSELAASQHNVFTRRQAASRGITRKQLHRLIESGVI
                     VAIESGGLVVLPEQGRPSWHQRVIGAVLDHPGTVASHTTAARLDGVDGFEVIDDIELS
                     VLRPRVVRIPGVTVHRVTQLDACDIVRWGPIRCTNRARTLCDLGASIEPLMVERALDD
                     AVRRGASVRWIRSTADRLHGPGRPGTRHLLAALGRLEGGGRVRDSWFEKLVELCLDDP
                     TIGRFERQYELRDDRGRHVARFDLALPDVQLGIEAHSREHHFGDEARMSGEHRDQAAA
                     ELGWDVMYLGYGSIRSPNAVRERVRARVEARWHLMHPDGA"
     gene            complement(1090..2466)
                     /locus_tag="PKY13_13155"
     CDS             complement(1090..2466)
                     /locus_tag="PKY13_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HOT80933.1"
                     /translation="MKRAALVVAALLATACSGNDATKAPARESAPDFLVERLGGTTSA
                     REFRGRPFNQSARSLNNAEFRRFGTGALVFDAHFTAEQGLGPVFNEDSCLSCHLDGVV
                     FKEHTDDDPGPGLLLRLSVPGTSEHGGPVPEPTYGLQLQTSKVVGATAEGRLDVEWEY
                     RSGRYPDGTEYELRAPTFTVSDLAGGPLAADVLTSARIAPPMTGMGLLEAIPVASLEA
                     AADPEDADGDGISGRLNQVWDSASGTTMAGRFGWKAGQPTVRQQSVGALHDDMGITSP
                     DVPETCHNQGSLCTVEPGADDEPEMSATDMDDQVFYNRTIAVPIARNVDDAAVVRGAN
                     RFVDVGCSSCHTTTQRSGDDEIAGLAGQTFHPFTDLLLHDMGDGLADGRPEFDASGQE
                     WRTAPLWALGRRAETTGFSSFLHDGRARDFEEAILWHDGEGRASRRAFMALTRQERSD
                     LLAFLAAL"
     gene            complement(2463..4097)
                     /locus_tag="PKY13_13160"
     CDS             complement(2463..4097)
                     /locus_tag="PKY13_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT80934.1"
                     /translation="MSDLADFSLADAWETIADVVGDAPALYHQDIVRTWAEFEERSAR
                     LASVLLAHGVGHDSKVAHYLYNGPEYLEATFASFKARAVPVNVNYRYTDAELSYLLDN
                     SDAEAIVVDTTFAEHVERLLPSLPKVKLVVEVGGSDGSEDRGRVAWVGYEEAIAAAEP
                     APRIARSGDDLWFLYTGGTTGMPKGVMWPHRSLFGTGAATFKNFKLPLPATLGELADS
                     IRTIHAEHETIRLLPAAPLMHGTSAIASWGVMCAGGAINTLASRSFDPAELLEAVQRN
                     RVTNLTIVGDAFAKPIIAELERAESAGSPYELSSLRLVISSGVMWSQQTKDALLARAD
                     VLCADLLGSSEGVGFANSVAKRNRPAPTARFRLGEHAQVFTEEGVAVEPGSGERGLLA
                     VGGPIPIGYYKDPEKSAGTFRPFGGRVWSVPGDWATVDADGTITLLGRGSVCINTAGE
                     KVFPEEVEETLKTHAAVRDANVVGVPDDKWGQAVVAVVSFEPGASAATADLVAHCRET
                     LAGYKVPKRFEVVDVVRRGPNGKADYRWAASVASGA"
     gene            complement(4108..>5082)
                     /locus_tag="PKY13_13165"
     CDS             complement(4108..>5082)
                     /locus_tag="PKY13_13165"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HOT80935.1"
                     /translation="RAAWAPTGWVAARSQVELPAPAPTTTTTTTSTPGRSRRRPATGS
                     PRIDVGDDASVTIDLGVLTSSGESLEIAAPELCLWRAPTDNDGIKSGWMAGVGARGRW
                     LRWGLDRMAAVDVDVSRRRGAIVRTTTWEADEAVGPIRHRQAISIVDGAVSFAETVEI
                     PKELDDLPRVGIRFVLPETFEHLEWFGPGPGDSYPDRRALPVERWATTVTDTYVDHIV
                     PQEHGHRTDVRWVELSDGVPRRRGRQPSTVAIEVDRRFGFNASHFTVEDLTAATHSHE
                     LVPRPEVHVHVDIAHRGLGTAACGPDTHPRHLVGGGRHRWAWSLAVVR"
BASE COUNT          773 a         1783 c         1788 g          738 t
ORIGIN      
        1 cgggcgcgcc agcgcaccat cctcacgcgc cgtcgggatg catcaggtgc cagcgtgcct
       61 cgacccgcgc tcgcacccgc tcccggaccg cgttcggcga ccggatggat ccatacccca
      121 ggtacatcac gtcccaaccc agctcggcgg ccgcctggtc acggtgctca ccgctcatcc
      181 gcgcctcatc cccgaagtgg tgctcccggc tgtgcgcctc gatgccgagc tgcacatcgg
      241 gcagggcgag atcgaatcgc gccacatgcc ggccgcggtc gtcacgtagc tcgtattgac
      301 gctcgaagcg cccgatcgtc gggtcgtcga ggcacaactc gaccagcttc tcgaaccacg
      361 agtcccgcac ccgaccgcca ccctccagcc ggccgagggc agcgaggagg tggcgggtac
      421 cgggacgccc cggcccgtgc aggcgatcgg cggtcgatcg gatccagcgc acgctcgcgc
      481 cccgcctgac agcatcatcg agggcccgct cgaccatcag cggttcgatg ctggcgccga
      541 ggtcgcacaa cgtgcgagcc cggttggtgc accgaatcgg gccccaccgc acgatgtcgc
      601 acgcgtcgag ctgcgtcacg cggtggacgg tgaccccggg tatccgaacg acccgcggtc
      661 gcagcacgct gagctcgatg tcgtcgatga cctcgaagcc gtcgacgccg tcgagacgag
      721 ctgctgtggt gtgggatgcg acggttcccg gatgatcgag caccgcaccg atgacgcgct
      781 ggtgccacga cggccggcct tgttcgggga gcacaacaag tccgccgctc tcgatcgcca
      841 cgatgacacc ggactcgatg agtcggtgca gttgcttccg ggtgattcct cgggaggcag
      901 cctggcgacg ggtgaagacg ttgtgttgag aggctgcgag ctcggacaca acgctcaagg
      961 cgttggtcgg cattcgacga gtatgcgcgc gagccctgac agccagcgca cctgacggcg
     1021 gagtgtgagg ttggtgcgcc agcggtcccg aacgggcgcg ccagcgcacc atcctcggac
     1081 gggcgaccgc tacagcgctg cgaggaaggc gaggaggtcg gagcgttcct gacgggtcag
     1141 cgccatgaag gcccggcggc tcgcccggcc ctcgccgtcg tgccagagga tcgcctcctc
     1201 gaagtcgcgg gcgcgaccgt cgtggaggaa cgaactgaac cccgtggtct ccgcacgccg
     1261 accgagcgcc cacagcgggg cggtgcgcca ctcctgcccc gacgcgtcga actcgggacg
     1321 accgtcggcc agaccgtcgc ccatgtcgtg gaggagcagg tcggtgaacg ggtggaacgt
     1381 ctggccggcc agaccggcga tctcgtcgtc gcccgaccgc tgcgtcgtcg tgtggcacga
     1441 cgagcacccg acgtcgacga accggttggc gccacggacg acggccgcgt cgtcgacgtt
     1501 gcgggcgatc ggcaccgcga tcgtccggtt gtagaacacc tggtcgtcca tgtcggtggc
     1561 ggacatctcg ggttcgtcgt cggctccggg ttcgacggta cacagcgagc cctggttgtg
     1621 gcacgtctcg gggacgtcgg gcgaggtgat gcccatgtcg tcgtggagcg cccccacgct
     1681 ctgttggcgc acggtcggtt ggcccgcctt ccaaccgaag cgcccggcca tcgtggtgcc
     1741 cgaggcactg tcccagacct gattgagtcg acccgagatg ccgtcgccgt ccgcgtcctc
     1801 ggggtcggcc gcggcctcga gcgacgcaac cggaatcgcc tcgagcagcc ccatccccgt
     1861 catcggcgga gcgatgcgcg ccgacgtcag gacgtcggcg gcgagcggac caccggcgag
     1921 atcggagacc gtgaacgtgg gagcgcgcag ctcgtactcg gtgccgtcgg gatagcggcc
     1981 ggagcggtac tcccactcga cgtcgagccg accttcggcg gtggccccga ccaccttgct
     2041 cgtctgcagc tgcagcccgt aggtcggttc gggaaccggc ccgccgtgct cggacgtgcc
     2101 gggcacgctg aggcggagca gcaggccggg gccggggtcg tcgtcggtgt gttccttgaa
     2161 cacgacgccg tcgagatggc agctgaggca ggagtcctcg ttgaacaccg gtccgagccc
     2221 ctgctccgcg gtgaagtgcg cgtcgaagac cagcgccccg gtgccgaacc gacggaactc
     2281 ggcgttgttg agcgaccgcg ccgactggtt gaacggacgt ccacggaact cccgcgccga
     2341 cgtcgtgccg ccgaggcgct cgaccaggaa gtcgggtgcc gactcacgcg ccggcgcctt
     2401 cgtggcatcg ttccccgaac aggccgtcgc gagcaacgcg gcgacgacca gtgccgcccg
     2461 cttcatgcgc ccgaggccac ggacgcggcc caccggtaat cggccttgcc gttggggcca
     2521 cgccgcacca cgtcgaccac ctcgaaacgc ttgggcacct tgtatccggc cagcgtctcg
     2581 cggcagtgtg cgacgaggtc ggcggtggcc gcggacgcac cgggttcgaa cgagacgacc
     2641 gctaccacgg cctggcccca cttgtcgtcg gggaccccca ccacgttggc gtcgcgtacc
     2701 gcggcgtggg tcttgagcgt ctcctcgacc tcttcgggga acaccttctc gccggcggtg
     2761 ttgatgcaca ccgaaccccg gccgagcaag gtgatcgtgc cgtcggcatc gacggtcgcc
     2821 cagtcgccgg gcaccgacca cacccggccg ccgaagggcc ggaacgtgcc ggcggacttc
     2881 tcggggtcct tgtagtagcc gatggggatg ggaccgccga cggccagcag gccacgctcc
     2941 ccggagcccg gctcgaccgc cacgccctcc tcggtgaaca cctgggcatg ctcaccgagc
     3001 cggaaccgcg ccgtgggtgc gggacggttg cgcttggcga cggagttggc gaagcccact
     3061 ccttcggagc tgcccaacag gtcggcgcag agaacgtcgg cacgagccag cagcgcgtcc
     3121 ttcgtctgct gcgaccacat gacgcccgag ctgatcacga gccggaggct cgacagttcg
     3181 tagggcgagc cggcggactc cgcacgctcg agctcggcga tgatcggctt ggcgaacgcg
     3241 tcgccgacga tcgtcaggtt ggtgacccgg ttgcgttgga ccgcctcgag caactcggcg
     3301 ggatcgaagc tccgcgatgc cagcgtgttg atcgccccgc ccgcacacat gacgccccag
     3361 ctcgcaatcg cggacgtgcc gtgcatcagc ggcgccgcgg gcaacagccg gatggtttcg
     3421 tgctccgcgt ggatggtgcg gatggagtcg gccagttcgc cgagggtcgc cggcaggggc
     3481 aacttgaagt tcttgaacgt cgcggctccg gtgccgaaca gcgaccggtg cggccacatg
     3541 acgcccttgg gcatccccgt cgtgccgccg gtgtagagga accacaggtc atcgccagac
     3601 cgggcgatac ggggggccgg ctcggcggcg gcgatggcct cctcgtagcc gacccacgcc
     3661 accctgcctc gatcctcgct cccatccgaa ccaccgacct cgacgacgag cttcaccttc
     3721 ggaagggagg gcaggaggcg ctcgacgtgc tcggcgaagg tcgtgtcgac gacgatcgcc
     3781 tcggcgtcgg agttgtcgag caggtaggag agctcggcgt cggtgtaccg gtagttgacg
     3841 ttgaccggga ccgcccgggc cttgaacgac gcgaaggtcg cttcgaggta ctcgggcccg
     3901 ttgtacagat agtgggccac cttcgagtcg tgaccgacac cgtgcgcgag caggaccgac
     3961 gccagtcggg cggaccgctc ctcgaactcg gcccaggtac ggacgatgtc ctggtggtac
     4021 aaggccggcg cgtcgccgac gacatcggcg atcgtctccc acgcgtcggc gaggctgaag
     4081 tctgcaaggt cgctcatgcg gcgctcatca tcgcacgacc gcgaggctcc aggcccaacg
     4141 gtggcgtcca cccccgacga ggtgccgcgg atgggtatcg ggcccgcacg ccgccgtgcc
     4201 gaggccacga tgggcgatgt cgacgtgcac gtgaacctcc ggccgcggca ccagctcgtg
     4261 ggagtgggtc gccgccgtga ggtcctcgac ggtgaagtga gacgcgttga acccgaaccg
     4321 ccgatcgacc tcgatggcga ccgtcgacgg ctggcgccct cggcggcggg gaactccgtc
     4381 ggacagctcg acccagcgga catcggtgcg gtggccgtgc tcctggggaa cgatgtggtc
     4441 gacgtacgtg tcggtcaccg tcgtggccca gcgctcgacg ggaagggcgc gccggtccgg
     4501 gtagctgtcg ccgggcccgg gaccgaacca ctccaggtgc tcgaacgtct cgggcagcac
     4561 gaaccggatg ccgacccgcg gcaggtcgtc gagttccttc gggatctcga ccgtctcggc
     4621 gaaggacacc gccccgtcga cgatcgagat cgcctggcgg tgccggatcg gcccgaccgc
     4681 ctcatcggcc tcccacgtgg tcgtccggac gatcgccccc cggcgacggc tgacgtcgac
     4741 gtcgacggcg gccatccggt cgaggcccca tcgcagccac cgaccgcggg caccgacgcc
     4801 ggccatccaa ccggacttga tgccatcgtt gtcggtgggc gcccgccaga ggcacagctc
     4861 cggcgcggcg atctcgaggg actcccccga cgacgtgagc acaccgaggt cgatcgtgac
     4921 cgaggcgtcg tcgcccacgt cgatgcgggg ggatcccgtg gccggccgac gacggctccg
     4981 gcccggggtc gatgttgtgg ttgtggttgt ggtcggagca ggggccggga gctcgacctg
     5041 ggaacgcgcc gccacccacc ccgtcggcgc ccacgcggca cg
//
LOCUS       DAOSOA010000247         5033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16795_length_5033_cov_5.726195, whole genome shotgun sequence.
ACCESSION   DAOSOA010000247 DAOSOA010000000
VERSION     DAOSOA010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16795_length_5033_cov_5.726195"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKY13_13170"
     CDS             complement(<1..833)
                     /locus_tag="PKY13_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80936.1"
                     /translation="MELPITDHTGSNDPYPTDAQLVAATLGGDRQALGHIYDRYSDRI
                     YTMCVHMLGDRDEAADVCSEVFVVAFGRLDQLRDPARLRPWMYAICRHEVFRRTKRRG
                     RVRLVDEVSDMDRAAEYNDFDGVDAVDAEANQAGLALVVREAAAGLDDRDRLVLELHL
                     QGVEGDDLAAALGTSTSTSYQHVHRMRERLERSIGALLVARQGRSDCDELDRLLSSWD
                     GRFSVLWRKRVARHVDGCDICEHRRAGVPAALFGTAGASPLLVTPTSVRDRVLATAVV
                     GG"
     gene            complement(902..1495)
                     /locus_tag="PKY13_13175"
     CDS             complement(902..1495)
                     /locus_tag="PKY13_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80937.1"
                     /translation="MSNTTTRSQNLPTVDRVRLGVVRTIAVGLLASGALVVFAGAASA
                     KPIVPDLDIDDITVATLPPVTIPPKTIPPFTIPPVVLDPGSPTTVPPTVPPVVVNPTT
                     TVKPPVTIPPVIVNPTVPPTTVAPEPTPDPTDPTTVPDPTVPADVEGATETAAGPESL
                     AVTGNDSNLPIIGASLLGAGLVIVGGMAYDRRRKAQG"
     gene            complement(1655..3112)
                     /locus_tag="PKY13_13180"
     CDS             complement(1655..3112)
                     /locus_tag="PKY13_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT80938.1"
                     /translation="MSIDAQVCIGGEWFDGGNGSYDIINPATEEVVGRAPQGSVDQSI
                     AATAAAAEAFDAWSRTSPEERANLLEKAADLYDARKDEFAPLVQAETGATMRVAKTMQ
                     VPQVSARFRRYARGAMESRLIPLEPQVMPTTALAPGGIIGGVGHRAPVGVVSCITSYN
                     FPVVNMAGKIGPALAMGNTVVVKPAPQDPLAVIKLVEIMNEAGFPPGVINVTCGTDIA
                     ASEALVSSPHVDMISFTGSTGVGRRIGAVAGGEMKRLLLELGGKGACIVYDDADLKAA
                     IGGISSVWAFHSGQICTAPTRVIAQRGIYDQLVAGLTQAAKHLTVGDPLERTTVVGPV
                     ITDVHRGRIEGYVQSARDEGAEIVVGGERPDFDKGFYVAPTLIANATNQMTAVREEIF
                     GPVVVVVPFDDDEEAIHIANDSDFGLYSYVFSGDTGRAWATAQRLRSGNVGINSLQRN
                     HEAPFGGFKQSGVGRDGGSFGLHAYSEMQSIIWPG"
     gene            complement(3144..>3389)
                     /locus_tag="PKY13_13185"
     CDS             complement(3144..>3389)
                     /locus_tag="PKY13_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT80939.1"
                     /translation="STYYYRQIYGCFFDDEYGLENIEKCGVNNICFETDYPHSDSTWP
                     HSKDTAIKLMGNLPDDIVYKLVRGNAIEMLGLDFDRA"
     assembly_gap    3390..3489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3490..4085)
                     /locus_tag="PKY13_13190"
     CDS             complement(<3490..4085)
                     /locus_tag="PKY13_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT80940.1"
                     /translation="MENFPKLISVDDHVIEHANVWQDRLPAKYRDVGPRVVQERGHMQ
                     FVGGVFSYEPADDGDLCDWWLYEDKKIPQTRLSAAAGFDRDEVKVVGITYEEMRKGCW
                     DPVARLADMDLNYTEAQMCFPSFPRFCGQTFKEAQDMELADLCVKAYNDWMVEEWCGG
                     SDGRLIPLIIVQLWDAELAAQEVYRNAERGVRATCFSE"
     gene            complement(4139..4531)
                     /locus_tag="PKY13_13195"
     CDS             complement(4139..4531)
                     /locus_tag="PKY13_13195"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOT80941.1"
                     /translation="MIDTEPPVDSAMRAGVPVGEDGSRMIPLRRWLMAVTILVLNLVD
                     VIVTKAILRHGGSEANPIMAPIMDHPAYPLILKTIVALGVGALLLASPRDSKLADRSV
                     MIVIVAYVCVMGWNFGILLQASGLHSPS"
     gene            complement(4610..>5033)
                     /locus_tag="PKY13_13200"
     CDS             complement(4610..>5033)
                     /locus_tag="PKY13_13200"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOT80942.1"
                     /translation="RVGTVRAVTMRYASLPETTSLEHAEVHRSTRHLRAVSARIERVL
                     GERSFRLWALLTLNALDLLTTAAVLALGGTESNPAMQGVVEHWWKPILVKGVVLGLMW
                     LVVLRTPVSSRLADLGLLLAWVFYAGVVLWNTLLLVNY"
BASE COUNT          798 a         1602 c         1743 g          790 t
ORIGIN      
        1 ccgccgacga ccgccgtggc gagcacccga tcgcggaccg aggtcggcgt gacgaggagc
       61 gggctcgcgc ccgcggtacc gaacagcgcg gcgggcaccc cggcgcggcg gtgttcgcag
      121 atgtcgcagc catcaacgtg ccgggcgacc cgcttgcgcc acaggacgct gaagcgcccg
      181 tcccacgacg acaggagtcg gtcgagctcg tcgcagtcgg acctcccctg ccgcgcaacg
      241 agcagtgccc cgatcgagcg ttccagtcgc tcgcgcatgc ggtggacgtg ttggtaggag
      301 gtcgacgtcg acgtaccgag ggcggcggcg aggtcgtcgc cctcgacccc ctgcaggtgg
      361 agctcgagca ccagccggtc gcggtcgtcg aggccggcgg cggcctcgcg gacgacgagc
      421 gccaaccctg cctggttcgc ttcggcgtcg acggcatcga ccccgtcgaa gtcgttgtac
      481 tccgctgctc gatccatgtc actcacctcg tccaccagcc ggactcggcc ccggcgcttg
      541 gtccgccgga acacctcgtg ccggcagatc gcgtacatcc acggccgcag ccgggccgga
      601 tcccgcagct ggtcgagccg gccgaacgcc accacgaaca cctcgctgca cacgtccgcc
      661 gcctcgtcgc gatcgcccag catgtgcaca cacatggtgt agatccggtc gctgtagcgg
      721 tcgtagatgt gacccaacgc ctgccgatcg ccgccgagtg tggcggcgac cagctgggcg
      781 tcggtcgggt acgggtcgtt cgagcccgtg tggtcggtga tcggcagctc catgtcgcac
      841 ccttacggac ggctcggcgc agtcttgcac cgggcgactc ggtgaatcag ccctgcgtga
      901 atcagccctg cgccttgcga cgccggtcgt aggccatgcc gccgacgatc acgaggccgg
      961 cgccgagcag cgatgctccg atgatcggca ggttcgagtc gttgccggtc acggcgagcg
     1021 actccgggcc ggcggccgtc tcggtggcgc cctcgacgtc agcgggaacg gtggggtcgg
     1081 gcaccgttgt cgggtccgtc ggatccggcg tgggctcggg ggcgacggtc gtcggcggca
     1141 cggtggggtt cacgatgacg ggtgggatcg tgacgggggg cttcacggtc gtggtcgggt
     1201 tcacgacgac cggcgggacc gtgggcggca cagtggtcgg tgaacccggg tcgaggacga
     1261 ccggcgggat ggtgaagggg gggatcgtct tcggcgggat cgtgacgggc ggcagcgtcg
     1321 ccacggtgat gtcgtcgatg tcgaggtcgg gcacgatcgg cttggccgag gcggcgccgg
     1381 cgaagacgac caacgctccg gaggcgagca gcccgacggc gatggtgcgg accactccga
     1441 ggcggacgcg atcgacggtg gggaggttct ggctgcgggt ggtggtgttc gacatggctg
     1501 gctcctgggt tccgaggcga cgggttgtcg ccgtacgggg gccaagagcg cgatcaccgc
     1561 gaggtctgac acttttccgc gaacttctcc gaaaactgct gcgagggtcg cgccggagcg
     1621 cgaccctcgc agccagatct ggagcgtgac tcggtcaacc cggccagatg atggactgca
     1681 tctcgctgta ggcgtggagg ccgaagctcc cgccgtcgcg cccgacgccc gactgcttga
     1741 agccgccgaa cggcgcctcg tggttgcgct gcagggagtt gatgccgacg ttgcccgagc
     1801 gcagccgctg ggccgtcgcc caggcgcgac cggtgtcgcc gctgaagacg tagctgtaca
     1861 ggccgaagtc gctgtcgttg gcgatgtgga tggcttcctc gtcgtcgtcg aaggggacga
     1921 ccacaacgac cgggccgaag atctcctcgc gcaccgccgt catctggttg gtggcgttgg
     1981 cgatgagggt cggggcgacg tagaagccct tgtcgaagtc ggggcgttcg ccgccgacca
     2041 cgatctcggc gccctcgtca cgggccgact gcacgtagcc ctcgatgcga ccgcggtgca
     2101 cgtcggtgat gacggggccg accacggtgg tgcgctcgag cgggtcgccg acggtgaggt
     2161 gcttggccgc ctgggtgagg ccggcgacga gctggtcgta gatcccgcgc tgcgcgatga
     2221 cgcgagtggg tgcggtgcag atctgaccgg agtggaaagc ccacaccgag ctgatgccgc
     2281 cgatggcggc cttgaggtcg gcgtcgtcgt agacgatgca ggcacccttg ccgccgagtt
     2341 ccaacagcag ccgcttcatc tcgccaccgg cgaccgcgcc gatgcggcga ccgacgccgg
     2401 tggaaccggt gaagctgatc atgtcgacat gcggcgacga caccagcgcc tcggaggcag
     2461 cgatgtcggt gccgcaggtg acgttgatga cgccgggcgg gaacccggcc tcgttcatga
     2521 tctcgacgag cttgatgacg gcgagcggat cctgcggggc cggcttcacc acgacggtgt
     2581 tgcccatcgc gagcgccggg ccgatcttgc ccgccatgtt cacgaccggg aagttgtacg
     2641 acgtgatgca gctgacgaca ccgacgggcg cacggtggcc gacgccaccg atgatgccgc
     2701 ccggagcgag cgccgtggtc ggcatcacct ggggctcgag ggggatgagc cgtgactcca
     2761 tcgcacccct ggcgtagcgg cggaaccgtg ccgacacctg ggggacctgc atcgtcttgg
     2821 cgacgcgcat ggtggcgccg gtttcggcct gcacgagcgg agcgaactcg tccttgcgag
     2881 cgtcgtagag gtcggcggcc ttctcgagca ggttggcccg ctcctcgggc gacgtccgcg
     2941 accatgcgtc gaacgcctcg gccgcagcag cggtggcggc aatcgactga tcgacagagc
     3001 cctgcggcgc acgaccgacg acctcttcag tggcggggtt gatgatgtcg taggagccgt
     3061 tgcccccgtc gaaccactcc ccgccgatgc acacctgcgc gtcaatggac atgtgttggt
     3121 cttccgttct cgtgtcggcg ggctcaggcc cggtcgaagt ccaagccgag catctcgatg
     3181 gcgttgccac ggacgagctt gtagacgatg tcgtcgggca ggttgcccat caacttgatc
     3241 gccgtgtcct tggaatgggg ccatgtgctg tcggagtgcg ggtagtcggt ctcgaagcag
     3301 atgttgttca caccacactt ctcgatgttc tcgaggccgt actcgtcgtc gaagaagcag
     3361 ccgtagatct ggcggtagta gtaggtggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnna tctcgctgaa gcaggtggcc cgcacaccgc gctcggcgtt gcggtacacc
     3541 tcctgggcag ccagctcggc gtcccagagc tgcacgatga tgagcgggat gaggcgaccg
     3601 tcggagccgc cgcaccactc ctcgaccatc cagtcgttgt aggccttcac gcagaggtcg
     3661 gccagctcca tgtcctgggc ttccttgaag gtctgaccgc agaaccgggg gaacgacggg
     3721 aagcacatct gcgcctcggt gtagttcagg tccatgtcgg cgagacgggc gaccggatcc
     3781 cagcagccct tccgcatctc ctcgtaggtg atcccgacga ccttgacctc gtcacggtcg
     3841 aacccggcgg cggcgctgag acgggtctgc gggatcttct tgtcctcgta cagccaccag
     3901 tcacacagat cgccgtcgtc ggcgggctcg tagctgaaca cccctccgac gaactgcatg
     3961 tggccgcgct cttggaccac acgggggccg acgtcgcggt acttggcagg aagacggtcc
     4021 tgccagacgt tggcgtgctc gatgacgtgg tcatcgacgg agatgagttt cgggaagttc
     4081 tccatgcggc cgatcgtacc cacagacctg acggtgtgtc agaaacgcgg aaacgacgtc
     4141 acgacgggct gtggagcccc gatgcctgca acaggatccc gaagttccag cccatcacgc
     4201 agacgtaggc gacgatgacg atcatcaccg aacgatcggc cagcttcgag tcccgcggcg
     4261 acgcgagcag cagcgctccg acgccgaggg ccacgatcgt cttgaggatc aacggatacg
     4321 cggggtggtc catgatcggg gccatgatcg ggttggcctc ggagccgccg tggcggagga
     4381 tcgctttggt gacgatgacg tcgacgaggt tgaggacgag gatggtcacc gccatcagcc
     4441 accgacgaag cgggatcatg cgcgagccgt cctcgccgac cgggactccc gcacgcatgg
     4501 cactgtccac cggtggttcg gtgtcgatca ctcccaggtc cttccgacga cttccgtcga
     4561 ccgtacggac cagcaccgct cgccacaagt cgagatttac tgaaatgcgt caatagttaa
     4621 cgaggaggag ggtgttccac agcaccacgc ccgcgtagaa cacccacgcg aggagcaatc
     4681 ccagatcggc cagacgagac gacaccggcg tccggagcac caccagccac atgagcccga
     4741 gcacgacccc cttcacgagg atcggtttcc accagtgctc gaccaccccc tgcatggcgg
     4801 ggttcgattc ggtgccgccg agcgcgagca cggccgcggt cgtcaggaga tcgagggcgt
     4861 tgagggtgag cagcgcccac agccggaacg atcgctcgcc cagcacacgc tcgatccggg
     4921 cactgaccgc ccggagatgc cgcgtggacc ggtgcacctc agcgtgctcg aggctggtgg
     4981 tctccggcag cgaggcgtag cgcatggtca cggcccggac ggtaccgaca cga
//
LOCUS       DAOSOA010000248         5015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16869_length_5015_cov_6.292742, whole genome shotgun sequence.
ACCESSION   DAOSOA010000248 DAOSOA010000000
VERSION     DAOSOA010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16869_length_5015_cov_6.292742"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKY13_13205"
     CDS             complement(<1..798)
                     /locus_tag="PKY13_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80943.1"
                     /translation="MTDHPDPRGSGGPSDSSPDPDMTSDQLDELVGRLRDVPAPATSV
                     RAAHIAAALAAFDDLPALGAEDTTDTPTTAPLAPVVSLDPRRQRRRRAAQRWAAAAGL
                     VLVVGLGIGLATQQRGSSDDMATSASGSDASSDQQLSTERTADAVAPEAATASPGSQD
                     VPPNPAILQLGDVADVSALRSALDAARGAHTVTTIADATIDMAPADDGRAAGGSASTG
                     SAEAAKAPAPVDASCLDSLPPGSLVVAEATVAGRPVVIVETTTGSEGP"
     gene            complement(795..1382)
                     /locus_tag="PKY13_13210"
     CDS             complement(795..1382)
                     /locus_tag="PKY13_13210"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT80944.1"
                     /translation="MTIDRDQELAWASAAQAGDRAAMELLLACLYDHVHAVCRRICAN
                     DADADDTTQDALISIVRALPTFDGRSAVRTWAHRIAANSALDELRRRGRRPIPSDDQS
                     EATEPRTDAPGLADRVTDRLDLDAALAQLAPEFRAAVVLRDVVGMEYADIATTLGVPV
                     GTVRSRIARGRRHLAGLLGIKGNRSAPAGVTLEEP"
     gene            complement(1379..>1822)
                     /locus_tag="PKY13_13215"
     CDS             complement(1379..>1822)
                     /locus_tag="PKY13_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80945.1"
                     /translation="VAAATPFDPMGDTGQENNEQAPRAIDRDPATVWSTEGYNDQTFG
                     TKTGVGLILRTDTPARIASLRIEGSTGWKGAVYVTDTDPSAAAGPPESGGIPIDMASS
                     PTEVDLGNATGSYVLIWITDLGPASGRHRVEIAELSLSGRSEAEK"
     assembly_gap    1823..1922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1923..2676)
                     /locus_tag="PKY13_13220"
     CDS             complement(<1923..2676)
                     /locus_tag="PKY13_13220"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT80946.1"
                     /translation="MGEQSGRASSTVGRVIADRYELVAPLGTGGMAQVWEANDRLLSR
                     RVAVKLLHRHLADDPAFVQRFRREAVAAARLVHPNIVAVYDTVSTDECEAIVMELVDG
                     ETLRSRLDEDGPLSERKVRQLGIELAGALDCAHRNGIVHRDIKPANILITDDGSVRLA
                     DFGIAKAEEDPDLTVAGTLVGTAAYLAPEQVGGGRIDQRSDLYALSTVLYEAVSGSIP
                     FRGDSPAATALARLHHDPIDLATSMTGLDPAFA"
     gene            complement(2687..4819)
                     /locus_tag="PKY13_13225"
     CDS             complement(2687..4819)
                     /locus_tag="PKY13_13225"
                     /inference="COORDINATES: protein motif:HMM:NF039636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HOT80947.1"
                     /translation="MTSTLLRRLTGMTALILLVVATLVPAGSAAAQPPDDLADAGVSI
                     VVDSVSPWVDAEGTWSAALQVIGAPADAKITYSVRQPPVGTEADVRDALAKSREGDDE
                     PKVMRAAVSENLADVTDAAGTTTLQIAIRSGSGDRDRVKIPNSGVYPVVITVASADGT
                     ALERTTLFLNRLPAAEENDAGRYRLGIVLQQPRFGGFDDDGQVEISPALRSSVAAITD
                     TLAAADGLPVRVDLSPESLVALTQSETTGDLQLAERLRDELGDAAVMRVPWADLHLEG
                     WATSGTLPEVQTSLIDGQQALFARLQRPVEVRVWPPDPTVGPSSVELFSKLGVTTLLV
                     EPGQLTESKAPTGESGVSRPFRVLGTGGSTITGVSLDPELQQLLGTTSDSPALLAHQM
                     ITQMVGTWLADDQARGSILRIDESSDPDVVAALLDILGNAGDDAPLEVVDPADVAALP
                     PITVRQGGRDAAWDRELEAPAETPRVTGVAQRLRTARPLVDDYAAIVPRGDAMAAREA
                     IVIQRSLDRRVTPGVQETLLDTAITAMESDLAKISASKPRSLTLTSRSTSVPLRFTND
                     LGRPIRVKLRLQSPRLVFTDGAEQVLTLNPGLNRFDVKVDVRTSGQFVMQADLLAPDS
                     DRVLASTRQRIRSRTFSGVGLMLSGGALLFLVIWWSRTLRRTHRTPDDPNQPDEADPN
                     GAADTPDGDVPRALVPDDDHTRAAPSPR"
     gene            complement(4816..>5015)
                     /locus_tag="PKY13_13230"
     CDS             complement(4816..>5015)
                     /locus_tag="PKY13_13230"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT80948.1"
                     /translation="VHPDHSRRGIGAALVVDSLLWLRRRRGRMMLVNTQESNANALAL
                     YQHLGFTLEPEHLTVMAWSPV"
BASE COUNT          661 a         1615 c         1879 g          760 t
ORIGIN      
        1 cggcccctcg gaccccgtgg tcgtctcgac gatcacgacg ggtcgacctg ccaccgtggc
       61 ctcggcgacg acgagcgaac cgggcgggag cgagtcgagg caggacgcgt cgacgggggc
      121 cggagccttt gccgcctcgg cggacccggt cgaggccgac ccgccggctg ctctgccgtc
      181 gtccgcaggc gccatgtcga tcgtggcgtc ggcgatggtg gtcaccgtgt gggcgccacg
      241 agcggcgtcc agcgcggatc gcagcgcgct gacatcggca acgtccccga gctgcaggat
      301 cgccgggttc ggcgggacgt cctggctccc gggtgatgcc gtcgccgctt cgggggcgac
      361 cgcgtcggcc gtacgttcgg tcgagagctg ctggtcggaa ctcgcgtcgg aacccgaggc
      421 gctcgtggcc atgtcgtcgg agctgcctcg ctgctgtgtt gcgagtccga tgccgagccc
      481 gacgacgagc acgagtccgg cggcggcggc ccaccgctgt gcagcccggc ggcgtcgctg
      541 ccggcggggg tcgagcgaca cgacgggggc gagcggtgcc gtggtgggag tgtccgtcgt
      601 gtcctccgcg ccaagcgctg gcaggtcgtc gaaggcggcg agcgccgcgg cgatgtgggc
      661 ggcgcggacc gacgttgccg gggccggcac gtcgcggagc cgaccgacga gctcatcgag
      721 ctggtcggac gtcatgtcgg ggtccggcga tgagtcgctc ggccctccgg agccgcgggg
      781 atcggggtgg tcggtcatgg ttcttccaat gtgacgccgg cgggtgctga tcggttcccc
      841 ttgatgccga ggagccctgc caggtgacgc cgaccgcggg cgatgcggga ccgcacggtc
      901 cccacgggaa caccgagcgt cgtcgcgatg tcggcgtact ccatccccac gacgtcgcgg
      961 agcaccacgg cggctcggaa ctccggcgcg agctgggcca gcgccgcgtc gaggtcgagc
     1021 cgatcggtga cgcgatcggc gagccccggt gcgtcggttc gcggctcggt ggcttcggac
     1081 tggtcgtcgc tggggatcgg tcgccggccg cggcgccgca actcgtcgag cgcactgttc
     1141 gccgcgatgc ggtgtgccca ggtgcgcacg gcggagcggc cgtcgaacgt cggcagcgcc
     1201 cggacgatgg agatgagcgc atcctgggtg gtgtcatcgg cgtcggcgtc gttggcacag
     1261 atccgtcgac agaccgcatg gacgtggtcg tagaggcagg cgagcaacag ctccatggcg
     1321 gcgcggtcgc cggcctgcgc cgcgctcgcc caggcaagct cctggtcccg atcgatcgtc
     1381 acttctcggc ttcggaccgg cccgagagcg agagctcggc aatctcgacg cggtgccggc
     1441 cggacgccgg gccgaggtcg gtgatccaga tcagtacgta ggagcccgtt gcgttgccga
     1501 ggtcgacctc ggtgggggac gacgccatgt cgatcgggat gccgccggat tccggcgggc
     1561 ctgcggcggc gctgggatcg gtgtcggtga cgtacaccgc gcctttccag ccggtcgacc
     1621 cctcgatccg gagcgacgcg atccgggcgg gagtgtcggt tcggaggatg agcccgacac
     1681 cggtcttggt gccgaacgtc tgatcgttgt acccctcggt gctccacacc gtcgctgggt
     1741 cgcgatcgat cgcccggggc gcctgctcgt tgttctcctg gcccgtgtcc cccatcggat
     1801 cgaacggcgt cgcggctgcc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nncggcgaag gccgggtcga gcccggtcat cgatgtcgcc aggtcgatgg ggtcgtggtg
     1981 gagccgggcg agcgccgtcg ccgccggtga gtcgccgcgg aaggggatgc tgccgctgac
     2041 ggcctcgtac agcacggtgc tcagcgcata caggtcgctg cgctggtcga tccggccccc
     2101 gccgacctgc tcgggggcga ggtaggcggc ggtgccgacg agcgttcctg cgaccgtgag
     2161 gtcggggtcc tcctcggcct tcgcgatacc gaagtcggcg agacgcaccg agccgtcgtc
     2221 ggtgatgagg atgttcgccg gcttgatgtc gcggtgcacg atgccgttgc ggtgcgcgca
     2281 gtcgagcgca ccggcgagct cgatgcccag ctgccgcacc ttccgttcgg agagcgggcc
     2341 gtcctcgtcc agacgactgc ggagggtttc gccgtcgacg agttccatga cgatcgcttc
     2401 gcactcgtcg gtgctcaccg tgtcgtagac ggcgacgatg ttgggatgca cgaggcgggc
     2461 ggcggccacc gcctccctcc ggaagcgctg cacgaaggcc gggtcgtcgg ccaggtgccg
     2521 gtgcaggagt ttcacggcga cccgccgcga aagcaggcga tcattggctt cccacacctg
     2581 ggccatgccg ccggtgccga gcggtgccac cagctcatac cgatcggcga tcacacgacc
     2641 caccgtcgac gaggcccgac cggactgctc acccacgccg accacgctac ctgggggagg
     2701 gcgcggccct cgtgtggtcg tcgtcgggca ccagtgctcg cggaacgtcg ccgtcgggag
     2761 tgtcggcggc cccgttggga tcggcctcgt cgggttggtt cggatcgtca ggcgtgcggt
     2821 gcgtgcggcg gagcgtgcgt gaccaccaga tgacgaggaa gaggagcgcg ccacccgaca
     2881 gcatcaggcc gacgccgctg aacgtccggg atcggatgcg ctggcgcgtg gaggccagga
     2941 cacggtcgga gtccggcgcg agcaggtcgg cctgcatcac gaactggccc gaggttcgca
     3001 cgtcgacctt gacgtcgaac cggttgaggc ccgggttgag cgtcagcacc tgttcggcgc
     3061 cgtcggtgaa gaccagtcgg gggctctgca gtcgcagttt cacgcggatg gggcggccca
     3121 gatcgttggt gaaccgcagc gggaccgagg tgcttcgcga ggtcagggtc agcgaacgcg
     3181 gcttcgacgc cgagatcttc gcgaggtcgg actccatcgc ggtgatggcg gtgtcgagga
     3241 gcgtctcctg gacccccggg gtgacgcgac ggtcgaggct tcgttggatg acgatcgcct
     3301 cgcgggcggc catcgcgtcg ccgcggggaa cgatcgcggc gtagtcgtcg acgagggggc
     3361 gggccgtcct caatcgctgg gcgaccccgg tgacgcgagg ggtctcggcc ggcgcctcga
     3421 gctcgcggtc ccacgcggcg tcacgaccgc cttggcgcac ggtgatgggc ggcagtgcag
     3481 cgacatcagc ggggtccacc acctcgaggg gtgcatcgtc accggcgttg ccgagtatgt
     3541 cgaggagggc cgccacgaca tcggggtcgg aggactcgtc gatgcggagg atcgagccgc
     3601 gcgcctggtc gtcggcgagc caggtgccga ccatctgggt gatcatctgg tgcgcgagca
     3661 gcgccgggct gtccgacgtc gtgccgagca gttgttgcag ttcggggtcg agtgacactc
     3721 cggtgatggt gctgccaccg gtgccgagta cccggaaggg gcgggacaca ccggactcac
     3781 cggtgggcgc cttcgactcg gtgagctggc ccggctcgac gagcagcgtc gtgaccccga
     3841 gcttcgagaa cagttcgacg ctgctgggcc cgacggtcgg gtcgggcggc cacacccgga
     3901 cctccaccgg ccgctgcagg cgggcgaaca gcgcctgctg gccgtcgatg agggaggtct
     3961 gtacctcggg cagggtccct gacgtggccc agccctcgag gtggaggtcg gcccacggaa
     4021 cccgcatcac cgcagcgtcg ccgagttcgt cacggagtcg ctcggccaac tgcaggtcgc
     4081 cggtcgtctc gctctgggtc agcgccacga gcgactccgg gctgaggtcg acccgcaccg
     4141 gcaggccatc ggccgccgcg agggtgtcgg tgatggcggc gaccgaactg cggagcgctg
     4201 gggagatctc gacctggccg tcgtcgtcga agccgccgaa ccggggttgt tggagcacga
     4261 tgcccagtcg gtagcggccg gcgtcgttct cctcggccgc cgggagtcgg ttcaggaaca
     4321 gggtggtgcg ttcgagcgcc gtcccgtcgg cggatgcgac ggtgatgacg acggggtaga
     4381 cgccgctgtt ggggatcttc acccggtcgc ggtctccgga acccgatcgg atcgcgatct
     4441 gcagcgtcgt cgtgccggcg gcatcggtga catcggcgag gttctcgctc acggcggcgc
     4501 gcatcacctt gggttcgtcg tcgccctcgc gactcttcgc gagcgcgtca cggacatccg
     4561 cctcggtacc gaccggcggc tggcggacgc tgtaggtgat cttggcgtcg gcaggcgcgc
     4621 cgatcacctg cagcgcagcc gaccaggtcc cttcggcgtc gacccacggg ctcacgctgt
     4681 cgaccacgat cgaaaccccg gcgtcggcga ggtcgtcggg tggttgcgcg gccgccgagc
     4741 cggcgggaac cagggtcgcg acgacgagca ggatcagggc ggtcatgccc gtgagccggc
     4801 gcaggagtgt cgaggtcaca ccggactcca cgccatgacc gtgaggtgct ccggttcgag
     4861 ggtgaacccc agatgctgat agagcgccag cgcgttcgcg ttcgattcct gagtgttgac
     4921 gagcatcatc cgacctcgtc gccgtcgcag ccagaggagg gagtcgacca cgagcgctgc
     4981 gccgatgcca cgtcgggagt ggtcggggtg cacgg
//
LOCUS       DAOSOA010000249         5009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16897_length_5009_cov_5.788656, whole genome shotgun sequence.
ACCESSION   DAOSOA010000249 DAOSOA010000000
VERSION     DAOSOA010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16897_length_5009_cov_5.788656"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..2198)
                     /locus_tag="PKY13_13235"
     CDS             complement(3..2198)
                     /locus_tag="PKY13_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HOT80949.1"
                     /translation="MEALFSDNTTATTDRAGNELDAAGRATRQRLLSGLNTAQAAAVT
                     SDALALRIIAGAGSGKTRVLTRRIARRVAEGEVDPRRVLAVTFTRKAAAELRQRIDHL
                     GLRGGINAGTFHAIAYTQLRQRWEERGINPPELLDRKVGFVARLMRGGSSTLPLDVVS
                     EIEWAKARMISAGDYPAQAAIANRRVPIAPEAVADIYARYEETKLGRRMVDFDDLLRL
                     ATRDIEADPEYAAARRWRFRHLFVDEFQDVNPLQFRLLQAWVGPQVDLCVVGDPNQTI
                     YAWNGADSQFLDQFPTLMPDAETIELNENYRSTPQILGLANTVLSQTPSRRFDLVPNR
                     PDGPVPNIVSFDTETAEARGIARLARDAHRPGSRWSDQAVLVRTNAQLTVFEEAFSAA
                     GIPFRSRGGGRLLDQPEIKDILTRLRRGGGSLQVHLSDIESQLASQSPAQSPAADAAT
                     GDDEPLPVPPDESVAPVVGGLTDERSANVAELLRLGREFLDLEPEGTSASFLSWLNSA
                     LRNDNSGGATDAVDLATFHAAKGLEWPIVYVAGLEEGLVPIHYANTEEASNEETRLLY
                     VALTRAERQLTLTWAEKRSFGTRSQNRKRSPLLDTVGIAIQLLRESTSTIDLTASVAA
                     VAAERAKLRASDGGSRPRRLPGVKGDAPALSPEELVLLDRLKKWRIEHARASDVPAFV
                     IFNDATLHEVARQQPSTARELTAISGIGTVKAQRFGPDLLRLVAEHNDR"
     gene            2292..2978
                     /locus_tag="PKY13_13240"
     CDS             2292..2978
                     /locus_tag="PKY13_13240"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT80950.1"
                     /translation="MAFPKKHLYNDESIVLDRYPHWWYLAPRAFLLVLASAAGIFALT
                     YDFAADSNLEKPIQYVAAALVVIALGAFVVRLIGWRATNFVVTTERCIYRSGVLHKTG
                     IEIPLDRINTVFFSQNLFERILHAGDLGIESAGEQSRQNFSDITNPVEVQNTIYRQME
                     ENENRKYDRIGSEARQAAAAAVGQPQAAAQPTQVITVAEQLEKLADLHQRGVITTEQF
                     EAQKAHLLGA"
     gene            3149..>5009
                     /locus_tag="PKY13_13245"
     CDS             3149..>5009
                     /locus_tag="PKY13_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOT80951.1"
                     /translation="MTAVGTHRYTAEIRRTTHGVAHIQGASIPDVVFGQGYACAADHL
                     PTIADQVLKVRSERARFFGRGEGDRHLNSDLGYLALDVAAWAERMAATQPPEVVAVVE
                     AYGAGINRWLEEHGTSALPEWCRDAEWVRPVSTLDMFGLFADAALMASGRNLAQFLGS
                     ARAPGATDDPTSTPTDPLVPDDRPGSNGWALGRAVTDNGRGIVVANPHFPWYGDGRFW
                     ECHLTVPGELDVYGVSLVGAPAVHIGFNRDVAWTHTFSAGHRFVVYQLDLVPGDPTSY
                     RYGDEVRSMTSRDVSVAVLGDDDTIGSVHRTLWSTHHGPMVDIPLLGWSETAGFALRD
                     VNDGNDRFLGQYLAMDRATSVGDLRAAVHEHQGLPWVNVIAADRHGDVWYCDASPTPN
                     LSDEVSHAFDRAVDEDPLTALFFSQRVALLNGSDPATEWIDDGARQPGAVPPDRLPEL
                     TTDTVAFNSNDPYWVAHPELRVDRGPVLAGLYGRPLSPRTRMNATVLSGAAPTGPSGP
                     EGRWTRDDLERALLDNRSLLAEQLLDGVVDRCRSAGVVERGSSVFDLGAIADLLAGWD
                     RAFDLTSVGAVVWREFLGGFSNDDLRKAGPLYAEPFDPERATLTPSGLAPAPES"
BASE COUNT          733 a         1743 c         1769 g          764 t
ORIGIN      
        1 ggtcagcggt cgttgtgctc ggcgaccagg cggaggaggt cggggccgaa gcgttgcgcc
       61 ttgaccgtgc cgatgccgct gatcgccgtg agctcgcggg cggtcgacgg ttgctggcgg
      121 gcgacctcgt gcagcgtggc gtcgttgaag atcacgaacg ccggcacgtc ggaggcgcgg
      181 gcgtgttcga tgcgccactt cttcaagcgg tcgagcagga ccagctcctc gggcgacagc
      241 gccggtgcgt cgcctttgac cccgggcaag cggcggggac gggacccacc gtcgctcgct
      301 cggagcttgg cccgctcggc ggcgaccgcc gccaccgacg ccgtgaggtc gatggtcgac
      361 gtcgactcgc ggaggagttg gatcgcgatg ccgacggtgt cgaggagggg cgaacgcttg
      421 cgattctggg accgggtgcc gaacgagcgc ttctcggccc aggtgagggt cagctgccgc
      481 tcggcgcggg tcagcgcgac gtacagcagc cgggtctcct cgttgctcgc ctcctcggtg
      541 ttggcgtagt ggatgggcac gaggccctcc tcgaggccgg ccacgtagac gatcggccac
      601 tcgaggccct tggccgcgtg gaacgtggcc aggtcgacgg catcggtggc gccgccgctg
      661 ttgtcgtttc gcaacgccga gttgagccac gacaggaacg atgcgctggt gccttcgggt
      721 tcgaggtcga ggaactcccg cccgagccgc agcaactcgg cgacgttggc gctccgttcg
      781 tcggtgagtc cgccgaccac cggggcgacc gactcgtcgg gcgggaccgg gagcggctcg
      841 tcgtcaccgg tcgcagcgtc ggccgcaggc gactgggccg gcgactgcga tgccaactgc
      901 gactcgatgt cggacaggtg cacctggagg gacccgccgc cacgacgcag gcgcgtgagg
      961 atgtccttga tctcgggctg gtcgaggagc cggccgccgc cccgactgcg gaacgggatg
     1021 cccgctgcgc tgaaggcctc ctcgaacacc gtgagctgcg cgttggtgcg gacgagcacc
     1081 gcctggtcgg accagcgcga gcccggccgg tgggcatctc gggcgagtcg ggcgatgccg
     1141 cgggcctcgg ccgtctcggt gtcgaaggag acgatgttgg gcaccgggcc gtcgggacgg
     1201 ttgggcacca ggtcgaagcg acgcgacggc gtctgggaca gcaccgtgtt ggcgagaccg
     1261 aggatctgcg gggtcgaccg gtagttctcg ttgagttcga tcgtctcggc gtcgggcatg
     1321 agcgtgggga actggtcgag gaactgggag tcggcgccgt tccacgcgta gatcgtctgg
     1381 ttggggtcgc cgaccacaca gaggtcgacc tgggggccca cccaggcctg caggagccgg
     1441 aactggagcg ggttgacgtc ttggaactcg tcgacgaaga gatggcggaa gcgccaccgg
     1501 cgagccgcgg catactcggg gtcggcctcg atgtcgcggg tcgcgaggcg gagcaggtcg
     1561 tcgaagtcga ccatacgacg accgagcttg gtctcctcgt agcgggcgta gatgtcggcc
     1621 accgcctcgg gagcgatggg cacccggcgg ttggcgatcg ccgcctgcgc cgggtagtcg
     1681 ccggccgaga tcatgcgcgc cttggcccac tcgatctcgc tgacgacgtc gagcggcaac
     1741 gtggacgagc cgccccgcat gaggcgcgcc acgaacccga ccttgcggtc gagcagctcc
     1801 ggcgggttga tgccgcgctc ttcccaccgt tgccggagct gggtgtaggc gatggcgtgg
     1861 aacgtgccgg cgttgatgcc gccgcgcagt ccgagatggt cgatgcgctg gcgcagctcg
     1921 gcggcagcct tgcgggtgaa cgtgacggcg agcactcgcc gtggatcgac ctcgccctcc
     1981 gccacccggc gggcgatgcg gcgggtgagg accctcgtct tgccggaccc ggcgccggcg
     2041 atgatgcgca gcgccagcgc gtcggacgtg acggcggcgg cctgtgccgt gttgaggccc
     2101 gagagcaggc gttggcgggt ggcgcgaccg gccgcgtcga gctcgttgcc ggcacgatcg
     2161 gtggtcgcgg tggtgttgtc gctgaacagg gcctccacgc cgaggaccct aaccgggccc
     2221 actgacagcg agcgcagcac caaccgggcg catcagagct tcgcggcgga tgcgtcgcta
     2281 gccttgacga cgtggcattc cccaagaagc acctgtacaa cgacgagtcg atcgtgctcg
     2341 accgctaccc ccattggtgg tacctcgcgc cgcgggcctt cctcctggtg ctggccagcg
     2401 ccgccggcat cttcgcgttg acctacgact tcgccgccga ctccaacctc gagaagccga
     2461 tccagtacgt cgcggccgca ttggtggtga tcgcgctcgg cgcattcgtc gtccgcctca
     2521 tcgggtggcg agcgaccaac ttcgtcgtca cgaccgaacg ctgcatctac cgttccggcg
     2581 tcctccacaa gacgggcatc gagatcccgc tcgaccgcat caacacggtg ttcttctccc
     2641 agaacctgtt cgagcgcatc ctccacgccg gcgacctcgg tatcgagtcc gccggtgagc
     2701 agtcccgtca gaacttctcc gacatcacca acccggtgga ggtgcagaac accatctacc
     2761 ggcagatgga ggagaacgag aaccgcaagt acgaccgcat cgggagcgaa gcccgtcagg
     2821 ccgcggcagc ggccgtcggc cagccgcagg cggcggccca gcccacccag gtcatcacgg
     2881 tcgccgagca gctcgagaag ctcgccgacc tccatcagcg cggcgtgatc accaccgagc
     2941 agttcgaggc ccagaaagct cacctgctcg gggcgtagac gacgcgtccc ccggggttcg
     3001 agggggaccg tggccgagcg aagtggtgcg tgagcgcgat ggaaacgtcg cgccagcgca
     3061 ccacttcggg cgccaccgac cgtgccgatc ggatcgggca tcgggcacgt tcgcccggtg
     3121 tggtcgcaac gagaactacg ttctcggcat gaccgccgtt ggaacgcacc gctataccgc
     3181 cgagatccgc cgcaccaccc acggtgtcgc ccacatccag ggagcgagca tccccgacgt
     3241 cgtcttcggg caggggtacg cgtgcgctgc ggaccacctg cccacgatcg ccgaccaggt
     3301 cctcaaggtg cgcagcgagc gggctcgatt cttcgggcgg ggcgagggtg accgccacct
     3361 gaactccgac ctcggctatc tcgccctcga cgtcgccgcg tgggcggagc gcatggccgc
     3421 cacccagccg cccgaggtcg tcgcggtggt cgaggcctac ggcgccggca tcaaccgctg
     3481 gctcgaggag cacgggacct ccgcccttcc ggagtggtgc cgcgacgccg agtgggtgcg
     3541 acccgtctcg acgctcgaca tgttcgggtt gttcgccgac gctgccctca tggcgtcggg
     3601 ccgcaacctc gcccagttcc tgggctcggc ccgcgccccg ggggcgaccg atgatccgac
     3661 gtcgaccccg accgacccgc tcgtgcccga cgaccggccc ggcagcaacg gatgggcact
     3721 gggccgagct gtgaccgaca acggtcgcgg catcgtcgtg gccaaccccc actttccctg
     3781 gtacggcgac ggccgcttct gggagtgcca cctgaccgtg cccggagagc tcgacgtcta
     3841 cggcgtctcc ctcgtgggag cgcccgccgt gcacatcggc ttcaaccgcg acgtcgcctg
     3901 gacgcacacc ttcagcgccg gccaccgctt cgtcgtctac caactcgacc tcgtccccgg
     3961 tgatcccacc tcgtaccgct acggcgacga ggtccggtcg atgacatcgc gagacgtcag
     4021 cgtcgccgtg ctcggggacg acgacacgat cggctcggtc caccggaccc tgtggtcgac
     4081 ccaccacggg ccgatggtgg acattccgct gctcggctgg tccgagaccg ccgggttcgc
     4141 gctgcgtgac gtcaacgacg gcaacgaccg attcctgggc cagtacctgg cgatggaccg
     4201 agccacgagc gtcggcgacc tgcgcgccgc cgtccacgaa caccagggcc tgccgtgggt
     4261 gaacgtgatc gccgccgatc gccacggtga cgtctggtat tgcgatgcgt cgccgacccc
     4321 caacctcagc gacgaggtct cccacgcgtt cgaccgtgca gtcgacgagg acccgctcac
     4381 ggcgttgttc ttcagtcagc gggttgcgct gctgaacggg tccgacccgg cgaccgagtg
     4441 gatcgacgac ggagcccgcc agcccggcgc cgttcccccc gaccgccttc cggaactgac
     4501 caccgacacc gtcgcgttca actccaacga cccctactgg gtggcgcatc ccgagctccg
     4561 cgtcgaccgg ggcccggtgc tggcggggct gtacgggcgc ccgttgtcac cccggacccg
     4621 gatgaacgcc acagtgctgt ccggtgctgc tccgaccggg cccagtggtc ccgagggccg
     4681 ctggacccgc gacgacctcg agcgtgccct gctcgacaac cggtcgctgc tcgccgaaca
     4741 gctgctcgac ggggtggtcg accgctgtcg ttccgccggt gtcgtcgagc gtggaagctc
     4801 ggtgttcgat ctgggcgcga tcgcggacct gctcgcaggg tgggaccggg ccttcgacct
     4861 gacgtcggtg ggtgcggtcg tgtggcggga gttcctcggc ggcttctcca acgacgacct
     4921 ccgcaaggcg ggtcccctct acgccgagcc gttcgacccc gaacgagcga cgctcacgcc
     4981 gagtggcctc gccccggccc ccgagtcgg
//
LOCUS       DAOSOA010000250         5000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16932_length_5000_cov_4.821436, whole genome shotgun sequence.
ACCESSION   DAOSOA010000250 DAOSOA010000000
VERSION     DAOSOA010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16932_length_5000_cov_4.821436"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKY13_13250"
     CDS             <1..223
                     /locus_tag="PKY13_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80952.1"
                     /translation="LGVQPEVGAQERFTVDWGVLLDQGVKNFREAPAGALGPAFAIAV
                     AGLAFGWFGDGLARIFNPRLTVRRRSSDS"
     gene            220..1176
                     /locus_tag="PKY13_13255"
     CDS             220..1176
                     /locus_tag="PKY13_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT80953.1"
                     /translation="MSGDAVLELRDLQVSFGAGKHEVTVVHDVSLRVERGEIVGVVGE
                     SGSGKTLTAMAATDLLPSGARRDASAWAFDGTDLLGADRNGRAAALGGKLGVVFQDPM
                     SSLNPARRVGSQLSDTSRFWGKRTRAQAHDDAVDRLRRVRIAAPERRVDDYPHEFSGG
                     MRQRAMIAMGLMATPQLIVADEPTTALDVTVQADVMTLLRELNEEQGTAVLLVSHNIA
                     LVAEICHRVVVMYAGRIVEELAAADLATGARHPYTRALIGSVPTLDADLSQPLPAIGG
                     APPDPGHLPPGCAFAARCPLATDRCSTERPPLVDGVACWEVS"
     gene            1176..2120
                     /locus_tag="PKY13_13260"
     CDS             1176..2120
                     /locus_tag="PKY13_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT80954.1"
                     /translation="MLHLDDISIRYPGSVADVVERVTLDIPEGAALGLVGESGSGKST
                     VARAIVGLTPVRAGSVTVAGTDVTNARGAALAHVRANVQMVFQDPNASLHPRMTIGDA
                     VAEAVLVRRQKLSGSYLDAEVGRLLDLVGLPARFLDRFPFELSGGQRQRVAIARALAP
                     QPKLLLLDEPTASLDVSIQATVLNLLRDLQRELGLTYLAISHDLAVMRYLCDRIAVME
                     LGSIKESGPATEMFAAPRHHYTAALLDAVPRLSGRTTERIVLRGDPSDASERPPGCSF
                     HLRCPAGPGAVEGHERCAVDVPALDGSPHAVACHFPLT"
     gene            complement(2113..3090)
                     /locus_tag="PKY13_13265"
     CDS             complement(2113..3090)
                     /locus_tag="PKY13_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT80955.1"
                     /translation="MRASRLVQLLLVLQRRGKVTAADLAAELEVSVRTIYRDVEALGM
                     AGVPVVTDSGPGGGVSLIGGYETRLTGLTGEEATAIGLAGVPTAAAELGLGSVLVAAQ
                     AKVDAALPPELRARAVRVRERFHVDAPGWFQRPTSVPMLPALSAAVWDGRSVELHYAG
                     RHGTVRRRVDPLGLVLKGANWYLVAGSRRPDRLRSFRVDRVRSVRPMDRAVERPESFD
                     LATAWGELQAGFEQTLRSFDVVIRMERADVPRLRHALPEPSASRALDAAAAADGDPVT
                     FVVQSESVEVALGELLRLGGRVEVLAPPTLREAIRATGAAIAAAHAGLR"
     gene            3155..3649
                     /locus_tag="PKY13_13270"
     CDS             3155..3649
                     /locus_tag="PKY13_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT80956.1"
                     /translation="MIDFNELSRIAPEVAGPIGERFAASGLGMLGTVRSNGAPRVSPI
                     EVGPYEGGLFVGMMPGSQKHLDVTRDPRCCLVTAIADRHDLGGEGKLYGALDLVTDPD
                     RSAAILRHHAEANGFEAEELSGSPMFEVLIDRAAWQRVVDDAWTTLSWRHGVDGVRRR
                     RRTN"
     gene            complement(3670..4524)
                     /locus_tag="PKY13_13275"
     CDS             complement(3670..4524)
                     /locus_tag="PKY13_13275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT80957.1"
                     /translation="MTDVDGQPDNTAVRTALWRALHVEIDAAPPVVDDVLGRELANPD
                     PGWRDRPDMHPQRTSGPRAAIVARARFVDDLVAEQIDAGVDQIVLLGAGLDTLAQRHP
                     GYGERAQFFEIDQPGTQAWKRRRLVEIGHAPPDWLHFVPVDFESGEDWWDGLRSAGFD
                     PGRPAVVSSTGVSMYLTKSATAATLRRLATLATGSTVVMTFFVPLDLADEADRPGLEM
                     SARGAAASGTPFVSFYTPDEIVGCALEAGFASARHIASGTLADRYFADRPDGLRPSTG
                     EDVLIAST"
     gene            complement(4521..4880)
                     /locus_tag="PKY13_13280"
     CDS             complement(4521..4880)
                     /locus_tag="PKY13_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80958.1"
                     /translation="MTTTEGIEGVFLTTHNWGKAAKFFQALGYELEFDTGHRSGQLRN
                     GSGAYVFIAEVPEDQPPSTQLVLTTTEANAAELDPIVDVVSTFADTHWGTREMVVRDP
                     DGRMWSLQAPGSTAGDR"
BASE COUNT          712 a         1773 c         1761 g          754 t
ORIGIN      
        1 tctcggtgtg caacccgagg tgggggcgca ggaacggttc accgtcgact ggggtgtgct
       61 cctcgaccaa ggggtgaaga acttccgcga ggcgcccgcc ggcgcgctcg gtcccgcgtt
      121 cgcgatcgcc gttgccggtc tggcgttcgg ttggttcggc gacggtctcg cccgcatctt
      181 caacccgcgg ctcactgtcc gacgccggag cagcgactcg tgagcggcga cgccgtgttg
      241 gagttgcgcg accttcaggt ctccttcggc gcagggaagc acgaggtgac cgtcgtgcac
      301 gacgtgagcc tgcgggtcga gcggggcgag atcgtcggag tggtcggcga gtcgggctcg
      361 ggcaagacgc tgaccgccat ggcggcgacc gacctgctcc cgtcgggcgc tcgccgggac
      421 gcgagcgcgt gggctttcga cggcaccgac ctcctcggcg ccgaccgcaa cgggcgcgcc
      481 gctgcgctcg gcggcaagct cggtgtggtc ttccaggacc cgatgtcgtc gctgaacccc
      541 gcccgtcgcg tcgggtcgca gctgtccgac acctcgcggt tctgggggaa gcggacccgg
      601 gcccaggccc acgacgacgc cgtcgaccgg ctccgtcggg tgcggatcgc cgcccccgaa
      661 cgtcgtgtcg acgactaccc ccacgagttc tccggcggca tgcggcagcg ggcgatgatc
      721 gccatgggcc tgatggcgac gccgcagctc atcgtggcgg atgaacccac gacggccctc
      781 gacgtgaccg tgcaagccga cgtcatgacg ctgctccgcg agctcaatga ggagcagggc
      841 acagccgtcc tgctcgtgag ccacaacatc gcgctcgtcg ccgagatctg ccatcgcgtc
      901 gtggtcatgt acgccggccg gatcgtggag gagctcgctg ccgccgacct cgccaccggt
      961 gcccgtcacc cctacacccg agccctcatc ggatcggtcc cgacgctcga cgccgacctg
     1021 tcgcagccgc tccccgccat cggcggcgcc ccgcccgatc ccgggcacct gccgcccggg
     1081 tgcgcgttcg cagcccgttg cccgttggca accgatcgct gctcgaccga acggccgcca
     1141 ctcgtggacg gcgtcgcctg ctgggaggtg tcgtgatgtt gcacctcgac gacatctcga
     1201 tccgctaccc gggcagcgtc gccgacgtgg tcgaacgcgt cacgctcgac attcccgaag
     1261 gcgccgcgtt gggtctggtc ggcgagtccg ggtcgggcaa gtcgacggtg gcacgggcca
     1321 tcgtcggcct cacgccggtg cgcgccggtt cggtcaccgt ggcgggcacc gacgtgacca
     1381 acgccagggg agcggcgctc gcccacgtcc gggccaacgt gcagatggtc ttccaggacc
     1441 cgaacgcgtc gctgcacccc cgcatgacca tcggcgacgc cgtcgccgaa gcggtactcg
     1501 tgcggcgcca gaaactctcg ggctcgtacc tcgacgccga ggtgggccgt ctgctcgacc
     1561 tggtgggcct gccggcgcgg ttcctcgatc ggttcccctt cgagctgtcg ggcgggcagc
     1621 ggcagcgggt cgccatcgcc agggccctgg caccacagcc gaagctgctg ctgctcgacg
     1681 agccgaccgc ctcgctcgac gtctccatcc aggcgacggt gctcaacctg cttcgcgacc
     1741 tgcagcgcga gctgggcctc acctacctcg cgatctccca cgacctcgcc gtgatgcggt
     1801 acctgtgcga ccgcatcgcc gtgatggagc tgggctcgat caaggaatcg ggcccggcga
     1861 cggagatgtt cgctgcgccc cgacaccact acacggcggc gctgctcgac gccgtgcccc
     1921 gcctgtcggg tcgtacgacc gagcgaattg ttctgcgggg cgatccctcc gacgcgtcgg
     1981 agcggcctcc gggctgcagc ttccacctcc ggtgccctgc cggcccggga gcggtcgagg
     2041 gtcacgagcg ctgcgctgtt gacgttcctg cgctcgacgg gtcgccgcac gccgtggcgt
     2101 gccactttcc cctcacctga ggcccgcatg ggccgccgcg atcgccgcgc cggtggcgcg
     2161 gatcgcctcg cgcagcgttg ggggtgccag cacttcgacc ctcccgccca gtcgcaggag
     2221 ctcgccgagc gcgacctcga cggactcgga ctgcacgacg aacgtgaccg ggtcgccgtc
     2281 ggccgctgcc gcggcgtcga gggctcggga tgccgacggt tcgggaagtg cgtgacgcag
     2341 ccgcggcacg tcggcccgct ccatccggat gacgacatcg aagctgcgta acgtctgctc
     2401 gaacccggcc tggagctcgc cccatgcggt cgcgagatcg aacgactcgg gtcgctcgac
     2461 ggcacggtcc atcggtcgca ccgaccggac gcggtcgacg cgaaacgatc ggaggcggtc
     2521 gggccgtcga gacccggcca cgaggtacca gttcgccccc ttgagcacga ggccgagcgg
     2581 atcgacgcga cggcgaaccg tgccgtgccg gccggcgtag tggagctcga cggaccgccc
     2641 gtcccacacc gctgcgctga gcgccggcaa catcggcacc gacgtcgggc gctggaacca
     2701 cccgggggcg tcgacatgga accgttcgcg tacccggacc gctcgtgccc gcagctccgg
     2761 cgggagcgcg gcgtcgacct tcgcctgggc cgcgaccagg acggacccca gtccgagttc
     2821 cgcggccgca gtcgggactc ccgccaaccc gatggcggtt gcctcctcgc cggtcaggcc
     2881 ggtgaggcgt gtctcgtagc cgccgatgag cgacaccccg ccgccgggcc cgctgtcggt
     2941 cacgacgggg acgccggcca tgccgagtgc ctcgacgtcg cggtagatcg tccggaccga
     3001 gacctccagt tcggcggcga ggtcggcggc cgtcaccttg ccgcgtcgtt gcagcaccaa
     3061 gaggagttgg acgagtcgac tggcacgcat gattcgacgg tattccacca tcactgacag
     3121 aaggtgtcag gaatgatccg gcaacatctc gggcatgatc gacttcaacg aactctcccg
     3181 gatcgctccc gaggtcgccg gccccatcgg cgagcgattc gcagccagcg ggctcggcat
     3241 gctcggcacc gtgcggtcga acggtgcgcc ccgtgtcagc ccgatcgagg tcgggccgta
     3301 cgaaggcggg ctgttcgtcg gcatgatgcc cgggtcgcag aagcacctcg acgtcacccg
     3361 cgacccccgc tgctgtctgg tcacggccat cgccgaccgc cacgacctcg gcggcgaggg
     3421 caagctctac ggcgccctcg acctcgtgac cgatcccgac cggtcggcgg cgatcctccg
     3481 tcatcacgcc gaggcgaacg gcttcgaggc cgaggagttg tccggctccc cgatgttcga
     3541 ggtgctgatc gatcgggccg cgtggcagcg cgtcgtcgac gacgcctgga ccacgctctc
     3601 gtggcgacac ggggtcgacg gtgtgcgccg acgtcggcgg accaactgag gacgagcggc
     3661 ggcgaccggt caggtcgagg cgatcagcac gtcctccccg gtcgacgggc ggagtccgtc
     3721 gggtcgatcc gcgaagtagc ggtcggcgag cgtgcccgat gcgatgtgtc gagcactcgc
     3781 aaacccggcc tcgagcgcac acccgacgat ctcgtcgggt gtgtagaagc tgacgaacgg
     3841 cgtacccgac gccgccgcgc cgcgggcgct catctcgagc ccgggacggt ctgcctcgtc
     3901 ggccaggtcg agcggcacga agaaggtcat gaccacggtc gaaccggtgg cgagggtggc
     3961 cagccgacgc aacgtcgccg ccgtcgccga cttcgtgagg tacatcgaga cgccggtgga
     4021 gctcacgacc gccggccgcc caggatcgaa gccggccgag cgcaggccgt cccaccagtc
     4081 ttcaccggac tcgaagtcga cgggcacgaa gtgcaaccag tcgggaggag catgcccgat
     4141 ctcgacgagc cgacggcgct tccacgcctg cgtgccgggt tgatcgatct cgaagaactg
     4201 ggcgcgctcg ccgtagccgg ggtggcgttg ggcgagcgtg tcgagcccgg ccccgagcag
     4261 cacgatctgg tcgacgccgg cgtcgatctg ttcggcgacg aggtcgtcga cgaaccgtgc
     4321 ccgggcgacg atcgccgcac gggggccgct ggtgcgctga gggtgcatgt cgggtcggtc
     4381 gcgccacccc gggtcggggt tcgcgagctc tcggccgagg acgtcgtcga ccaccggagg
     4441 tgccgcgtcg atctcgacgt ggagcgctcg ccagagcgcc gtcctgacgg cggtgttgtc
     4501 gggctgcccg tcgacgtcgg tcatcgatcg ccggccgtgg aaccgggagc ctggaggctc
     4561 cacatccgtc cgtcgggatc acggaccacc atttcccgtg tgccccagtg cgtgtcggcg
     4621 aatgtgctca cgacgtcgac gatggggtcg agttcggcgg cgttggcctc ggtcgtggtg
     4681 agcacgagct gcgtcgaggg tggctggtcc tcggggacct cggcgatgaa gacatacgcc
     4741 cccgaaccgt tgcggagctg gcccgaacga tgaccggtgt cgaactccag ctcgtagccc
     4801 aaggcctgga agaacttggc ggccttcccc cagttgtggg tcgtgaggaa cacaccctcg
     4861 attccttcgg tcgtcgtcat gtcatgtctc cttgtcggaa ccggccggtt ggatgccgcg
     4921 accgtcggcg aggtacgagc gcagtgcttc ggcaacgagt gccgagagcg agagctcgga
     4981 ctcgatggcg cggtgcttga
//
LOCUS       DAOSOA010000251         4992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_16955_length_4992_cov_6.210857, whole genome shotgun sequence.
ACCESSION   DAOSOA010000251 DAOSOA010000000
VERSION     DAOSOA010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16955_length_4992_cov_6.210857"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKY13_13285"
     CDS             <1..353
                     /locus_tag="PKY13_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80959.1"
                     /translation="AIGYTNASWTLKADLIAGYVTRLLNHLHNTGQDICVPHNVAAAA
                     PSGKMLDLSSGYLARAADRLPKSGDTYPWQVYQSYVKDYRATKRSDIRDDVMRFSRSA
                     KGSNRSVTSKEVGV"
     gene            355..1188
                     /locus_tag="PKY13_13290"
     CDS             355..1188
                     /locus_tag="PKY13_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT80960.1"
                     /translation="MENFDGKVAAITGAGSGIGRALAVELAKRGASLALSDVDEVGLA
                     ETVSLCEGYGVKVSSLVLDVSDRDAMFEWADQVADEHGKVNLIFNNAGVALGAVVESM
                     SIEDFEWLMSINFWGVVHGTQAFLPHLKASGDGHVINISSVFGLVSIPTQSAYNAAKF
                     AVRGFTDALRIELEIDDSGVSATTIHPGGIKTNIARNARVDEATAELSGDVGDPGDMF
                     EKIAMTKPSKAAKQILAAVEKNKRRALIGPDAKVFDLVSRLPAGFYQRLLINGAKRQR
                     K"
     gene            complement(1218..2669)
                     /locus_tag="PKY13_13295"
     CDS             complement(1218..2669)
                     /locus_tag="PKY13_13295"
                     /inference="COORDINATES: protein motif:HMM:NF005510.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT80961.1"
                     /translation="MASTDDGRSALAPVPAADVDRWDLDTDVIVVGLGCAGASAAIEA
                     AAAGVDVTVLEAAAMGGGTSAMSGGIIYLGGGTPVQQACGYEDSPENMEAFLTAACGP
                     GVDAAKVRAYCEDSVGHFHWLVEHGVPFEHSYFPEHDREPPDTSGLVFSGGEDSWPFN
                     ELATPVPRGHHPAHADAAGGFLMQRLIEAVAGSGATTVTDAAVQRLVVDDGVVVGVEM
                     TVDGEHRHARARRGVVLTAGGFTFNKTMVEEHCAPALRCNVPIGTPHDDGRAIRMAMG
                     VGATTIGMDHLEVGIPLAPPRSVVRGIIVNGAGERFMNEDTYAGRLGQEFLLRQDGRV
                     YWVHDDETFAVNIAGYKPRWVAETAAELETEIGLPAGSLEATLDQYNRHAELGEDPQH
                     HKAAEYVVPLRPPYGVVDLGVDRSYYATFTLGGLSTTPAGEVVSASGEPVGGLFAAGR
                     TTAGIAAAGYVSGISLGDGTYFGRRAGRSAAGN"
     gene            2852..3415
                     /gene="ahpC"
                     /locus_tag="PKY13_13300"
     CDS             2852..3415
                     /gene="ahpC"
                     /locus_tag="PKY13_13300"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HOT80962.1"
                     /translation="MALINTEILPFEATAFHNGEFVQVSDSDLKGKWSIVFFYPADFT
                     FVCPTELGDMADNYAAFQALGVEVYSVSTDTHFTHKAWHETSETIGKITYPMIGDPNG
                     VITTNFDVMRPGVGLADRGTFLIDPEGVIQFLEITAEGIGRNAAELLRKVKAAQYVAS
                     HPGEVCPAKWEEGEATLAPSLDLVGKI"
     assembly_gap    3424..3523
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3524..>4992
                     /gene="ahpF"
                     /locus_tag="PKY13_13305"
     CDS             <3524..>4992
                     /gene="ahpF"
                     /locus_tag="PKY13_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HOT80963.1"
                     /translation="NLTQPIELTATLDDGAKSTELNELLDEIASLSDKITYRRDGNAD
                     RAPSFTIDRLGTDVSVEFAGIPLGHEFTSLVLALLHVGGHPSKATQEVIAQVRELEGT
                     FEFETFVSLTCQNCPDVVQALNLMSTINPNIKSTTIDGAVFPAEAETRQVMAVPTVFL
                     NGEEWGSGRMTIEEIVNRLDSGAAAKEAERIDAKDAFEVLVVGGGPAGAAAAIYAARK
                     GINTGVVSERFGGQVLDTMAIENFISVPYTEGPKLATALEQHVHEYDVDVMNVQKAKR
                     LVPAAEAGGLVTVELESGAVVQSRSVVLSTGARWRSMNVPGENEYRNKGVTYCPHCDG
                     PLFKGKRVAVIGGGNSGVEAAIDLAGVVAHVTLIEFDSILRADEVLQRKLRSLPNVDI
                     IVSALTTEVRGDGEKVVGLVYTDRTDDASHEVALDGIFVQIGLLPNTEWLQGSIELSP
                     RGEIVIDDRGQTSLPGVFAAGDCTTVPYKQIVIAMGAGA"
BASE COUNT          823 a         1714 c         1599 g          756 t
ORIGIN      
        1 tggcgatcgg ctacacgaac gcctcctgga cgctgaaggc cgacctcatc gccggctacg
       61 tcacgcgcct gctcaaccac ctccacaaca cgggccagga catctgtgtc ccgcacaacg
      121 tggcggcggc agcgccatcg ggcaagatgc tcgacctgtc gtcgggctat ctcgcccgcg
      181 ccgcggaccg tttgcccaag tcgggcgaca cgtatccctg gcaggtgtac cagagctacg
      241 tgaaggacta ccgggcgacg aagcgcagcg acatccgcga cgatgtcatg cggttctcgc
      301 ggtccgccaa gggatcgaac cgctcggtga cgagcaagga agtaggggtc tgacatggag
      361 aacttcgacg gcaaggtcgc agcgatcacg ggggcgggtt cgggcatcgg ccgagcgctc
      421 gccgtcgagc tggccaagcg cggcgcgtcg ctcgcgttgt ccgacgtcga cgaggtcggg
      481 ctcgccgaga ccgtgagcct ctgcgagggc tacggggtca aggtgtcgtc cctcgtgctc
      541 gacgtgtccg accgcgacgc catgttcgag tgggccgacc aggtcgccga cgagcacggc
      601 aaggtcaacc tcatcttcaa caacgccggc gtcgccctcg gagcggtcgt cgagtcgatg
      661 tcgatcgagg acttcgagtg gctcatgagc atcaacttct ggggcgtcgt ccacggcacc
      721 caggcgttcc tgccgcatct caaggcgtcg ggcgacggcc acgtgatcaa catctccagc
      781 gtgttcggcc tcgtgagcat ccccacgcag tcggcgtaca acgctgcgaa gttcgccgtg
      841 cgcgggttca ccgacgcgct gcgcatcgag ctcgagatcg acgacagcgg cgtgtcggcg
      901 acgacgatcc accccggtgg tatcaagacc aacatcgccc gcaacgcccg cgtcgacgaa
      961 gcgaccgccg agctgtccgg cgatgtcggc gacccgggcg acatgttcga gaagatcgcg
     1021 atgacgaagc cgtccaaggc ggcgaagcag atcctcgcgg cggtcgagaa gaacaagcgc
     1081 cgcgcgctca tcggtcccga tgcgaaggtc ttcgacctgg tctcccgact cccggccggc
     1141 ttctaccagc gactgctgat caacggggcg aagcggcagc ggaagtagcg ccggggcttc
     1201 gaggacccct gaggagttca gttgcccgca gcgctccggc cggcgcggcg accgaagtag
     1261 gtgccgtcgc cgagggagat cccgctcacg tagccggcgg cggcgatgcc ggccgtcgtc
     1321 cggcctgcgg cgaagagccc cccaaccggt tcgccgctgg ccgacaccac ctcgccggcc
     1381 ggtgtcgtcg acagtccgcc cagcgtgaac gtggcgtagt agctgcggtc gaccccgagg
     1441 tcgacgacgc cgtagggggg ccgcaggggg acgacgtact cggccgcctt gtggtgctgg
     1501 gggtcctcgc cgagctcggc gtgacggttg tactgatcga gcgtggcctc gagcgagccg
     1561 gccggcaggc cgatctcggt ctccagctcg gccgcggtct ccgcgaccca ccggggcttg
     1621 tagccggcga tgttgacggc gaacgtctcg tcgtcgtgga cccagtacac gcgaccgtcc
     1681 tggcggagga ggaactcctg gccgagccgg ccggcgtagg tgtcctcgtt catgaaccgt
     1741 tcgccggcgc cgttcacgat gatgccgcgg acgacgctcc ggggcggagc gagcgggatg
     1801 ccgacctcga ggtggtccat gccgatcgtc gtcgcgccga cacccatcgc catgcggatg
     1861 gcccggccgt cgtcgtgcgg tgtgccgatc gggacattgc accgaagggc cggtgcgcag
     1921 tgctcctcga ccatcgtctt gttgaaggtg aacccgccgg cggtgagcac cacgccgcgt
     1981 cgggcccggg catgtcggtg ctcgccgtcg acggtcatct cgaccccgac gaccacgccg
     2041 tcgtcgacga cgagtcgttg caccgcggcg tcggtgaccg tcgtcgctcc cgagccggcg
     2101 accgcctcga tcagtcgctg catgaggaac ccgccggcgg cgtcggcgtg cgccgggtgg
     2161 tgaccacgag gcaccggggt cgccagctcg ttgaacggcc acgaatcctc gcccccgctg
     2221 aagaccagac ccgacgtgtc gggtggctca cggtcgtgct cgggaaagta ggagtgctcg
     2281 aaggggacgc cgtgctcgac gagccagtgg aagtgcccga cgctgtcctc gcagtaggca
     2341 cgaaccttcg ccgcgtcgac gcccggacca caggcggcgg tcaggaatgc ctccatgttc
     2401 tcgggggagt cctcgtaccc gcaggcctgt tgaacggggg tgccgccgcc gaggtagatg
     2461 atgccgcccg acatcgccga ggtgcccccg cccatcgctg ccgcctcgag cacggtcacg
     2521 tcgactccgg cggcagccgc ctcgatggcc gcgctggcac cggcgcagcc gaggccgacg
     2581 acgatcacgt ccgtgtcgag gtcccatcgg tcgacgtccg cggcggggac cggggcgagt
     2641 gcggaacgac cgtcgtcagt ggatgccatg ggccgggatt ctgccagagc gcgccggttc
     2701 ggtcggggct caggttgccg agtgtcgccc tcggcgatca gagattgaca gaaacgatcg
     2761 atcacatcga atcattcgat ttgaacgatc acacgggact gcctagattg acctcgtacc
     2821 cccagccatc caaccagcga ggaatctccc aatggcactg atcaacaccg aaatccttcc
     2881 cttcgaggcc accgccttcc acaacggcga attcgtccag gtgagcgact ccgacctgaa
     2941 gggcaagtgg tcgatcgtgt tcttctaccc ggccgacttc acgttcgtct gccccaccga
     3001 actcggcgac atggccgaca actacgcagc gttccaggcg ctcggcgtcg aggtgtactc
     3061 ggtctccacc gacacccact tcacccacaa ggcctggcac gagacctccg agaccatcgg
     3121 caagatcacg tacccgatga tcggcgaccc gaacggcgtc atcacgacca acttcgacgt
     3181 gatgcgtccc ggtgtcggcc tcgccgaccg tggcacgttc ctcatcgatc ccgagggcgt
     3241 catccagttc ctcgagatca ccgccgaagg catcggccgc aacgctgcgg agctcctccg
     3301 caaggtcaag gccgcccagt acgtggcctc ccaccccggc gaggtctgcc ccgccaagtg
     3361 ggaagagggc gaggccaccc tggccccgtc gctcgacctc gtcggcaaga tctgatcctg
     3421 cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagaacct cacccagccg
     3541 atcgagctca cggccaccct cgacgacggc gccaagtcga ccgagctgaa cgagctcctc
     3601 gacgagatcg cttcgctctc cgacaagatc acctaccgcc gcgacggcaa cgccgaccgt
     3661 gcaccgtcgt tcacgatcga ccgcctcggc accgacgtct ccgtcgagtt cgccggcatt
     3721 cccctgggtc acgagttcac gtcgctcgtg ctcgcactcc tccacgtcgg cgggcatccc
     3781 tccaaggcca cccaggaggt catcgcccag gtgcgcgagc tcgagggcac cttcgagttc
     3841 gagaccttcg tctcgctcac ctgccagaac tgccccgacg tggtgcaggc gctcaacctc
     3901 atgagcacca tcaaccccaa catcaagagc accacgatcg acggtgcggt gttccccgcc
     3961 gaggccgaga ctcgccaggt catggccgtg cccaccgtgt tcctcaacgg tgaggaatgg
     4021 ggttcggggc gcatgacgat cgaggagatc gtgaaccggc tcgactccgg cgccgccgcc
     4081 aaggaggccg agcgcatcga cgccaaggac gccttcgagg tgctcgtcgt cggcggtggc
     4141 ccggccggcg ctgcggccgc catctacgcc gctcgcaagg gaatcaacac cggtgtcgtg
     4201 tccgaacggt tcggtggaca ggtgctcgac accatggcca tcgagaactt catctcggtg
     4261 ccctacaccg agggcccgaa gctggcgacg gcgctcgagc agcacgtcca cgagtacgac
     4321 gtcgacgtca tgaacgtcca gaaggccaag cgactcgtgc cggccgccga ggccggcggg
     4381 ctcgtcaccg tcgagctcga gagcggcgcc gtggtgcaat cccggtcagt cgtgctgtcg
     4441 acgggtgccc gctggcgttc gatgaacgtg cccggtgaga acgagtaccg caacaagggt
     4501 gtgacctact gcccgcactg cgacggcccc ctcttcaagg gcaagcgcgt cgccgtcatc
     4561 ggcggcggca actcgggtgt ggaggccgcg atcgacctcg ccggtgtcgt cgcccacgtg
     4621 acgctcatcg agttcgactc catcctgcgc gccgacgagg tgctgcagcg caagctccgc
     4681 agcctcccga acgtcgacat catcgtcagc gccctcacga ccgaggtgcg tggtgacggc
     4741 gagaaggtcg tcggcctcgt gtacaccgac cgcaccgacg atgcgtccca cgaggtcgcc
     4801 ctcgacggca tcttcgtgca gatcggcctc ctgcccaaca ccgagtggct ccagggctcg
     4861 atcgagctct cgccccgcgg tgagatcgtc atcgacgatc gtggtcagac gtcgctgccc
     4921 ggcgtgttcg ctgccggcga ctgcaccacg gtgccgtaca agcagatcgt cattgccatg
     4981 ggcgccggtg cc
//
LOCUS       DAOSOA010000252         4969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17056_length_4969_cov_6.920228, whole genome shotgun sequence.
ACCESSION   DAOSOA010000252 DAOSOA010000000
VERSION     DAOSOA010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17056_length_4969_cov_6.920228"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PKY13_13310"
     CDS             <1..503
                     /locus_tag="PKY13_13310"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT80964.1"
                     /translation="AVSALARMGALSIPDLLAALADGAPEVRCRALGALPARWSGRVA
                     DDVHLLGMLHDDDAVVLEVACFVAGECEPAPPGVIERLIDICTGHDDPLCRESAVAAL
                     GSLGDPRAVGAVLSACRDKVTIRRRAVLALAAFEGDEVDSMLRSMADDVDWQVRQAAE
                     ELLAIE"
     gene            complement(526..1530)
                     /locus_tag="PKY13_13315"
     CDS             complement(526..1530)
                     /locus_tag="PKY13_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT80965.1"
                     /translation="MARDLAIDLGTANTLVYARGEGIVLNEPSVIALNSRNKEVLAMG
                     RDAWQMIGRTPSYIVAVRPLRKGAITDFEVTQEMIRLLLKRVGVNRFNRPRVVICVPS
                     AITAVERRAVIEATKRAGAADAHLLEQPMAAAIGAGLPINEPIANMVVDVGGGTAETA
                     LISLGGIVSLRAVRVGSFDLDAAIITYIRREYGIAIGEQTAEEIKMTIGSAERTDDEV
                     CAEVRGRELMSGLPRTVVLTPEEVREAIAEPVSTMVDSVLSCLGEAPPELAQDLISQG
                     INLVGGGALLKGLDIRIANASGVAVQMVRQPLEAVVLGAGRVIESYESLKVMFMDSRV
                     "
     gene            complement(1532..1735)
                     /locus_tag="PKY13_13320"
     CDS             complement(1532..1735)
                     /locus_tag="PKY13_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80966.1"
                     /translation="MNNALTFLGIAVAIAVVGGLVVALLHRNPESAGSDGVDQFQRIM
                     DALAPDDEDHPTPPDGAVGHNER"
     gene            complement(1732..2199)
                     /locus_tag="PKY13_13325"
     CDS             complement(1732..2199)
                     /locus_tag="PKY13_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOT80967.1"
                     /translation="MAPPHDGDDWVGLSADPLPLDVASSWAVLPSCGAVVTFSGHARD
                     HSPGRGGVTELEYEAYESQVAPALARLAAEMRRRWPSLGRIALLHRHGTLVVGDVAVV
                     VVVSSPHRDDAFEAARFGIDTLKSSTPIWKREVWADGESWGLEAQHLSAVDAS"
     gene            2337..3536
                     /locus_tag="PKY13_13330"
     CDS             2337..3536
                     /locus_tag="PKY13_13330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOT80968.1"
                     /translation="MFDTSGAGEDGWGMTPPPPAADRLWRHPSELAPAPTIAPTPHRA
                     EHRPHRFSLGTVVVASSLGALAMFGTLAATGLVGGTATTPQAERPSGITGAVTDATAS
                     YTTTSPPGVVSVRLSSASTTTFGAGIVFNSSGDVVTTLRATDPSGTPTGTVSVEVGDS
                     AWGEATIVGSDPVTGLTVLHPTATATAPHDAALVRSATVATPSLGQSVDLVRPGLGAI
                     GARFRSGTATRITSTNATIGTSGTSFVGLLTLTSPRPATSAEVGVDSTTGDVTALVVP
                     VDDNPEDEMAYAVPADLAIAIGRQIEATGSARHGTLAADLLQSPTDGLVVTATRSSEL
                     AVGDELLSVDGRMVHTNDDVLGALLGVLPGTEVNVVVRRDNRQRSVDADVVARIGDPV
                     ATTTTLP"
     gene            3591..4919
                     /locus_tag="PKY13_13335"
     CDS             3591..4919
                     /locus_tag="PKY13_13335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOT80969.1"
                     /translation="MNDLPGNDDESEPNPFGDMTAGLNQIFQLFGGGGLGGPGSAPDW
                     DRARQAAAALASEGGSEANVDPLVRIQFEQLARVAELQVAQVTGLTLSRTGAGLTVSP
                     VNRAQWAAATVDAYRPLFEKLAASLGRMMTSQLDEISSDELDEMSSMMPPGMSFDMSA
                     LMAGMSSFIGPMMLVTMAGSTVGQLGSRAFGSYDLPIPRPPSDELLVVATAIDDFGND
                     WSLPLDDLRLCICLAEMAHHAVLSQPHVRDRLNSLLAEHANGFEADSAALEERLGPID
                     LSDPESLGSLQNSLGNPEFMLSAIRSERQRQILPAIDTLVGCIEGYVDWVLDTIGAKL
                     LTSYPMVSEALRRRRVETAQASRFVERLFGLELTQDKIDQGSAFIDGVFARAGAPALA
                     ALWSDVEHLPTPNELQAPGLWLARVGLESDQPLPELDDDPGIPDFPDLDA"
BASE COUNT          759 a         1826 c         1660 g          724 t
ORIGIN      
        1 cagcggtgtc agcgctggcc cgaatgggcg ctctcagtat cccagacctc ctcgcggcgc
       61 tcgcggatgg tgctcccgag gtgcgctgcc gcgccctggg cgcgctgcct gcccgatggt
      121 cgggtcgagt cgccgacgac gtccacctgc tcggcatgct gcacgacgac gatgccgtcg
      181 tgctcgaggt cgcgtgcttc gtggccggcg agtgtgaacc cgccccgccc ggtgtgatcg
      241 agcgactgat cgacatctgc accggacacg acgacccact gtgtcgcgag agcgcggtgg
      301 ccgccctcgg ctcgctgggt gatccgaggg ccgtcggcgc cgtcctgtcc gcctgtcgag
      361 acaaggtcac gattcgccga cgggccgtgc tcgccctcgc cgcgttcgag ggtgatgagg
      421 tcgactcgat gctgaggtcc atggccgacg acgtcgactg gcaagtgcgt caggcggccg
      481 aggaactgct cgccatcgag tgacacctgc ggccggcggc cggtctcaga cccgcgaatc
      541 catgaacatg accttgagcg actcgtagct ctcgatcacc cggcccgcac cgagcaccac
      601 agcttcgagc ggctggcgaa ccatctgcac cgccacaccg gacgcgttgg cgatgcggat
      661 gtcgaggccc ttgagcagcg cgccaccgcc gacgagattg atgccctgac tgatgaggtc
      721 ctgcgccagc tccggcggtg cctcgcccag gcacgacagc accgaatcga ccatcgtcga
      781 caccggttcg gcgatggcct cgcggacctc ctcgggggtc agcacgaccg tccgaggcaa
      841 ccccgacatg agctcgcgtc cacggacctc ggcgcacacc tcgtcgtcgg tccgctcggc
      901 cgacccgatg gtcatcttga tctcctcggc ggtctgctca ccgatggcga tcccgtactc
      961 gcggcggatg taggtgatga tcgcggcgtc gagatcgaac gatccgacgc gcaccgcccg
     1021 caacgacacg atgccgccca aggagatgag tgccgtctcc gccgtgccgc cgcccacgtc
     1081 gacgaccatg ttggcgatcg gctcgttgat ggggagcccg gcaccgatcg ctgccgccat
     1141 cggctgctcg aggagatggg cgtcggcggc gccggcacgc ttcgtggcct cgatcaccgc
     1201 ccggcgctcg accgcagtga tcgccgacgg aacgcagatc accacacgag gccggttgaa
     1261 ccggttgacc ccgacgcgct tgagcagcag ccggatcatc tcctgggtca cctcgaagtc
     1321 ggtgatcgct cccttgcgaa gtggacggac ggcgacgatg tagctgggtg tccggccgat
     1381 catctgccag gcgtcgcgac ccatggccag gacttccttg ttgcgactgt tgagcgcgat
     1441 caccgacggc tcgttgagca cgatgccctc gccgcgcgcg tacacgagcg tgttggcggt
     1501 accgaggtcg atcgcgaggt cgcgggccac gtcagcgctc gttgtgcccg acggcgccgt
     1561 cgggtggggt ggggtggtcc tcgtcgtcgg gcgccaacgc gtccatgatg cgttggaact
     1621 ggtcgacccc gtcggagccg gcggactcgg ggttgcggtg caggagggcg acgacgaggc
     1681 cgcccacgac cgcgatcgcc acggcaatgc cgaggaacgt cagggcgttg ttcacgaggc
     1741 gtccaccgcc gagaggtgct gggcctcgag gccccaactc tcgccgtcgg cccacacctc
     1801 gcgcttccag atcggcgtcg acgacttcag cgtgtcgatc ccgaaccggg ctgcctcgaa
     1861 cgcgtcgtcc cgatgcgggg acgagaccac cacgacgacc gcgacgtcac cgacgacgag
     1921 ggtgccatgg cggtgcagga gcgcgatccg cccgaggctc ggccaacgac ggcgcatctc
     1981 cgcggcaaga cgggcgagcg ccggcgccac ctgggattcg tacgcctcgt actcgagctc
     2041 ggtcacgccg ccacggcccg gcgagtgatc gcgggcatga ccggagaacg tcaccacggc
     2101 accgcatgaa ggcaggacgg cccacgacga cgccacgtcg agagggagcg ggtcggcgct
     2161 gagaccgacc cagtcgtcgc cgtcgtgagg aggtgccacg accgacagcg tacgggagga
     2221 aggaggacct tgtagagccc gggccggccg gccgccgccg gacggcaccg gtgggagggg
     2281 ttcgatccga ccggccgcgg gcacatcacc cggtggcgtc ggtacagtcg cacccgatgt
     2341 tcgacacgag cggggccggc gaggacgggt ggggcatgac gccaccgccg ccggccgcgg
     2401 accggctgtg gcggcatccg tcggaactgg ccccggcgcc gacgatcgca ccgacccccc
     2461 accgcgccga gcaccgaccc caccggttct ccctcggcac ggtcgtcgtc gccagctcgc
     2521 tcggcgccct cgccatgttc ggcacgctcg cggccaccgg cctggtcggc ggcaccgcca
     2581 cgacaccgca ggccgagcgg ccgagcggca tcaccggtgc tgtaactgat gccacggcgt
     2641 cctacaccac gacctcaccg ccgggcgtgg tctcggtgcg cctcagcagc gcgagcacca
     2701 cgaccttcgg cgccggcatc gtcttcaaca gctccggtga cgtcgtgacc accctccggg
     2761 ccaccgatcc gtccggcaca ccgaccggca ccgtctcggt cgaggtcggc gactccgcct
     2821 ggggtgaggc gacgatcgtc ggcagcgacc cggtgaccgg cctcacggtc ctccatccga
     2881 ccgcgacagc gacagcaccg cacgacgccg cgctcgtgcg gagcgcgacg gtggcaacgc
     2941 cgtcgctggg ccagtccgtc gacctcgtac gtcccgggct cggggccatc ggcgcccggt
     3001 tccgcagcgg aacggcgacg cggatcacca gcacgaacgc cacgatcggc acatctggga
     3061 cgtccttcgt cggcctcctg accctcacct cgccccgccc tgccacctcc gctgaggtcg
     3121 gcgtcgacag cacgacgggc gacgtgaccg cgctggtggt gcccgtcgac gacaaccccg
     3181 aggacgagat ggcctatgcc gtccccgcgg acctcgccat cgccatcggc cgccagatcg
     3241 aggcaaccgg gtcggcgcgc cacggcacac tcgccgccga cctcctccag tcgccgaccg
     3301 acggcctcgt ggtcaccgcc acccgcagct ccgaactggc cgtgggcgac gagctgctct
     3361 ccgtcgacgg tcgcatggtg cacaccaacg acgacgtgct cggagcactt ctcggcgtgt
     3421 tgccgggcac cgaagtgaac gtcgtggtgc gccgcgacaa ccggcaacgc tcagtcgacg
     3481 ccgacgtcgt cgcccgcatc ggcgatccgg tcgccaccac caccacgctg ccctgatccc
     3541 ggcgccgagc gcgcggccgc tgcctcgtcc ggcgctccta cgatgcacgt gtgaacgatc
     3601 tgccgggcaa cgacgacgaa tccgaaccga atccgttcgg cgacatgacc gcggggctca
     3661 accagatctt ccaactgttc ggcggcggcg ggctcggagg gcccgggtcg gctcccgact
     3721 gggaccgcgc ccggcaggca gcagcggcgc tcgcgtccga gggcggatcg gaggccaacg
     3781 tcgatccgct cgtgcggatt cagttcgagc agctcgcccg ggttgcggag ctgcaggtcg
     3841 cccaggtcac cggcctcact ctttcccgca ccggtgccgg tctcacggtc tcgccggtca
     3901 atcgggctca gtgggcagcc gcgaccgtcg atgcctaccg gccgctcttc gagaagctgg
     3961 cggcgtcgct cggccggatg atgacgtcac agctcgacga gatctcctcc gacgaactcg
     4021 acgagatgag ctcgatgatg ccgccgggga tgagcttcga catgtcggcg ctcatggccg
     4081 gcatgtcgag cttcatcgga cccatgatgc tcgtgacgat ggccgggtcg accgtcggcc
     4141 agctcggcag tcgggcgttc ggctcctacg acctgccgat cccccgacca ccctccgacg
     4201 aactcctcgt ggtggcgacg gccatcgacg acttcggtaa cgactggagc ctcccgctcg
     4261 acgacctgcg cctgtgcatc tgcctggccg aaatggcgca ccacgccgtg ctgtcccagc
     4321 cccacgtccg cgaccgcctc aactcactgc tcgccgagca cgccaacggg ttcgaggccg
     4381 actcggcggc cctcgaggag cgcctcgggc cgatcgacct gtccgacccc gaatccctcg
     4441 ggtcattgca gaactcgctc ggcaacccgg agttcatgct cagcgccatc cggtcggaac
     4501 gccagcggca gatcctgccg gcgatcgaca cgctcgtcgg gtgcatcgag ggctacgtgg
     4561 actgggtcct cgacacgatc ggggcgaagc tcctgacctc gtacccgatg gtgagcgaag
     4621 cgctccgtcg gcgccgtgtc gagacggcgc aggcgagccg gttcgtcgag cgcctcttcg
     4681 ggctcgaact cacccaggac aagatcgacc agggctcggc attcatcgac ggcgtgttcg
     4741 cccgcgccgg cgctccggcg ctggcggctc tctggtccga cgtcgaacac ctgcccacgc
     4801 ccaacgagct gcaggctccc gggttgtggt tggcgcgggt cggactcgag tccgatcagc
     4861 cgctgccgga actcgacgac gaccccggca tcccggactt ccccgacctc gacgcatagc
     4921 gcagagcgat cccccggggt tcactgatct gacgaacggt tccttgcca
//
LOCUS       DAOSOA010000253         4963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17080_length_4963_cov_4.933374, whole genome shotgun sequence.
ACCESSION   DAOSOA010000253 DAOSOA010000000
VERSION     DAOSOA010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17080_length_4963_cov_4.933374"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKY13_13340"
     CDS             complement(<1..491)
                     /locus_tag="PKY13_13340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT80970.1"
                     /translation="MTDEDLRLEPSRFSPRDLFAEATAGVLARPGRAVLTALGTVLGV
                     AALVSTLGLAKTAGNQIVTRFDELDATDVKVSPRRGTLPLDSEARLTRLNGVAAAVTL
                     TEVDVKGRLVSSVPINDPMADNELPITVQAASPGAFRAVRASLRTGRFFDSAQDDLPV
                     AVLG"
     gene            complement(488..1171)
                     /locus_tag="PKY13_13345"
     CDS             complement(488..1171)
                     /locus_tag="PKY13_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT80971.1"
                     /translation="MTVVELTSVGRTFEGDPPVEALRSVDLRVDRGDYVSIVGPSGSG
                     KSTLLNILGLLDRPTSGTYRLDGLDVSGLTDAQRTAVRGQRIGFVFQAFHLLPHRTVL
                     ENVMLSQLYNRIDAKERRPRAQAALERVGLGHRVDFAPTTLSGGERQRVAIARALVTN
                     PAILLGDEPTGNLDSTTTGSILELFDELHSAGLTLIVITHDHEVSRRARRVVTMRDGV
                     LVDPTLADA"
     gene            complement(1185..2987)
                     /locus_tag="PKY13_13350"
     CDS             complement(1185..2987)
                     /locus_tag="PKY13_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOT80972.1"
                     /translation="MGRQRTLGAVALVSVLATSAAWYAGTRISSPAEARAKVKEPKAS
                     LITAPVERRQLSSNVVVRGDLAYDESTPVTLTASSETKAVVTKDPPAVGSQLTDGQLL
                     VEVSGRPVFVLQGDLPVFRNLARGVKGDDVRQLEAGLQRLGVDPGPVDGTFDEATERG
                     IAAFYLNAGYTVKEPSKEARDAVDEATSAVTSARQAARDAARAAGESSTTIKASERLQ
                     LESAVASASAALDSATAAASSSAKQAEAGLASLQNELVQAQAAKRQADAVYAAATEPG
                     AVDPDTGAPYTSTQLTELSNATLTAASAITAKAAEIDSAKAAADTDAREKFAAIGQAQ
                     RDLRIAQAAQLEGLKPSSDSSASSAVADAQRAVADAEKRLADAQAGVGVTVPLGELVF
                     VRALPRQINEVHLRRGDTASGEVVTISGADLKIRSMVVGADRALLTVGTKVKIDDSSL
                     DVHFEGVISEIADRPGTGASDNQGGADNSSDQNGGNGENGSSGSDDGEKYRVVITPGA
                     LPGDVDVETLAGVNFKITAPVKSTEGEVLAVPLAALSASADGSARVQVEDRPGSTRFV
                     TVQKGLAAQGFVEVSVAPGAKGTLDEGDQVVVGQ"
     gene            complement(2988..4124)
                     /locus_tag="PKY13_13355"
     CDS             complement(2988..4124)
                     /locus_tag="PKY13_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80973.1"
                     /translation="MNFSMHLSGRRVSRWLVAPAVALLVATGCGKESSGSEDSATVVE
                     FGNRQYGDASSSPLTKYFGYDDTEKLNASAREQNKKAERAIAECMHAEGFEYIPNLTA
                     GNFAAAPEIDLTQREYAEKWGFGITTTMQADGSPVEDAPGMASFDDTASQDPNQAIVD
                     GMSDSERAAYEQALYGASISSDLGDDGSGDGSEFDTEDLPEGCQSKAYGSDAEPSEAA
                     QKMDELYNDLSKRITADRRTKAATKEYRSCMADAGYPSVKTVDDVYEVMNKKAEELYA
                     ALSGDIGNSDVSASDDGSDEGGDDTSSAVTTVSGSGDGSTSTGIDPDKLAAVQKYERK
                     LAVAELPCRAEYQAAYEKVSAEYEQEFIDDNKSTLDEVKAEVVG"
     gene            complement(4185..4685)
                     /locus_tag="PKY13_13360"
     CDS             complement(4185..4685)
                     /locus_tag="PKY13_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOT80974.1"
                     /translation="MAGLEDLYREIIIDHYRSPRNRGELDTPPATRTEGFNPLCGDEI
                     VVYLQLGEGEGDDRVIEEIRIGGQGCSISQSSASMMSTAVNGKTIAEAREVMRAFKAM
                     MSIHESALDDSVEEPDIADIGELKLGDLEAMRGVVKFPVRIKCATLSWNTLDQGISEA
                     LTGDAV"
     gene            complement(4729..>4963)
                     /locus_tag="PKY13_13365"
     CDS             complement(4729..>4963)
                     /locus_tag="PKY13_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT80975.1"
                     /translation="RGGVFSLELAGVHPHNLSQVLDEHGVCVRAGHHCAKPLMKVLGV
                     GATARASVYIYNDEADVDALADSLEAAESFFAF"
BASE COUNT          695 a         1668 c         1744 g          856 t
ORIGIN      
        1 ccgagcacag cgacgggcag gtcgtcctgc gccgagtcga agaagcgccc ggtgcggagc
       61 gaggcgcgca ccgcccggaa cgcacccggc gatgccgcct gcaccgtgat gggcagctcg
      121 ttgtcggcca tcggatcgtt gatcggcacc gagctgacca accgaccctt cacgtccacc
      181 tcggtgagcg tgacggcggc ggcaaccccg ttgagtcgcg tgagccgtgc ctcggagtcg
      241 agcgggagcg tccctcgccg gggcgacacc ttcacatcgg tcgcgtcgag ctcgtcgaac
      301 cgcgtcacga tctggttccc ggccgtcttg gcgaggccca gtgtcgacac gagcgcggcg
      361 accccgagca cggtgccgag cgccgtgagt acggcgcgcc ccggtcgggc gaggacgccg
      421 gcggtcgcct cggcgaacag gtcgcgcggc gagaaccgtg acggttccag gcgcaggtcc
      481 tcgtcggtca tgcgtccgcc aaggtcgggt cgacgagcac accgtcgcgc atggtgacga
      541 cgcggcgtgc ccgccggctc acctcgtggt catgggtgat gacgatcagc gtgaggccgg
      601 cgctgtggag ctcgtcgaac aactcgagga tcgagcccgt ggtggtcgag tcgaggttgc
      661 cggtcggctc gtcgccgagg aggatcgccg gattggtcac gagggcgcgg gcgatcgcga
      721 cacgctgacg ttcgccacca gagagcgtgg tgggggcaaa atccacccgg tgccccaggc
      781 cgacgcgctc gagcgccgct tgggcgcggg gccgccgttc cttggcgtcg atgcggttgt
      841 acagctggct gagcatcacg ttctcgagca cagtgcggtg ggggaggagg tggaaggcct
      901 ggaagacgaa cccgatccgt tggccccgca cggcggtgcg ctgggcatcg gtcaggccgc
      961 tcacgtcgag gccgtcgagt cggtacgttc ccgacgtcgg acggtcgagc aggccgagga
     1021 tgttgagcag agtcgacttg cccgaacccg aggggccgac gatcgacacg tagtcacccc
     1081 ggtcgacgcg gaggtcgacc gatcgcagcg cttcgacggg tgggtcgcct tcgaacgtcc
     1141 gacccaccga ggtcaactcg acgaccgtca tggatgggcc gcgatcactg gcccacgacc
     1201 acttggtcac cctcgtcgag ggtgcccttc gcccccggag caaccgacac ttcgacgaaa
     1261 ccttgcgccg ccaggccctt ttgcaccgtc acgaatcgcg tcgatccggg acggtcctcc
     1321 acctggacgc gggccgaacc gtcggcgctc gccgacagcg ccgcgagcgg cacggccagg
     1381 acctcgccct cggtcgactt caccggtgcg gtgatcttga agttgacgcc ggcgagcgtc
     1441 tcgacgtcga cgtcgccggg gagggcgccc ggggtgatca caactcggta cttctcgccg
     1501 tcgtcggatc ccgaggagcc gttctcgccg ttcccgccgt tctgatcgct gctgttgtca
     1561 gctccgccct ggttgtcgct ggcgcccgtg ccaggccggt ccgcgatctc ggagatcacc
     1621 ccctcgaagt gcacgtccag gctgctgtcg tcgatcttca ccttcgtgcc cacggtgagc
     1681 agggcacggt ccgcgccgac caccatcgaa cggatcttga ggtcggcccc gctgatcgtg
     1741 acgacctcgc cggacgcggt gtcgccccga cggaggtgga cctcgttgat ctgacgcggc
     1801 agggcccgga cgaagacgag ttcgccgagg ggcaccgtca cgccgacccc ggcctgggcg
     1861 tcggcgagtc gcttctcggc gtcggccacg gcgcgttgtg cgtcggcgac ggccgacgag
     1921 gcgctggagt ccgacgacgg cttcagccct tcgagctgcg ccgcctgagc gatccggagg
     1981 tctcgctggg cctgaccgat ggcggcgaac ttctcacggg cgtccgtgtc ggccgcagcc
     2041 ttcgccgagt cgatctccgc cgccttcgcc gtgatcgccg acgccgcggt gagggtggcg
     2101 ttggacagct cggtgagctg cgtcgacgtg tagggggcgc cggtgtcggg atcgacggct
     2161 cccggttccg tcgctgcggc gtacaccgcg tcggcctggc gcttggcggc ctgcgcctgc
     2221 accaactcgt tctggagcga ggccaggccg gcctcggcct gcttggccga gctcgatgcc
     2281 gccgcggtcg cggagtcgag cgccgcggac gccgacgcca cggccgactc gagttggagt
     2341 cgctcggacg ccttgatcgt cgtgctcgac tcgcccgctg cccgagcagc gtcgcgtgcc
     2401 gcctgtcggg ccgaggtgac tgcggaggtc gcctcgtcga cggcgtcgcg cgcttccttg
     2461 gacggctcct tcacggtgta cccggcgttc aggtagaacg ccgcgatccc gcgttcggtg
     2521 gcttcgtcga acgtcccgtc cacggggccg ggatcgacgc cgagccgctg caaccctgcc
     2581 tccaactggc ggacgtcgtc acccttcacg ccgcgggcga ggttccggaa caccgggagg
     2641 tcgccctgca gcacgaagac cgggcgtccg ctcacttcca cgaggagctg tccatcggtc
     2701 agctgcgagc ccacggcggg cggatccttg gtgaccaccg ctttcgtctc cgacgacgcc
     2761 gtgagggtca ccggggtgga ctcgtcgtag gccaggtcgc cgcgcaccac gacgttggag
     2821 gagagctggc ggcgctcgac cggcgcggtg atgagcgacg ccttcggttc cttcaccttg
     2881 gcccgagcct cggcgggtga gctgatccgg gtgccggcgt accaggcggc gctggtcgcg
     2941 aggaccgaga ccaatgcgac ggcgccgagc gtgcgttgcc ggcccatcta cccgaccacc
     3001 tccgccttca cttcgtcgag ggtgctcttg ttgtcgtcga tgaactcctg ctcgtactcg
     3061 gccgacacct tctcgtaggc cgcctggtat tcggcgcggc acgggagctc ggcgacggcg
     3121 agtttgcgtt cgtacttctg cacagcggcg agcttgtccg gatcgatgcc cgtcgatgtc
     3181 gagccgtcgc cggagccgct cactgtcgtc acggcggagg atgtgtcgtc gcccccctcg
     3241 tcggagccgt cgtcggaggc cgacacatcg gagttgccga tgtcgccgct cagcgcggcg
     3301 tagagctcct cggccttctt gttcatcacc tcgtacacgt cgtcgaccgt cttgaccgac
     3361 ggatagccgg cgtcggccat gcacgagcgg tactccttcg tcgccgcttt cgttcgtcga
     3421 tccgcggtga tgcgcttgga gaggtcgttg tacagctcgt ccatcttctg ggcggcctcg
     3481 gacggctcgg cgtcggatcc gtatgccttg gactggcacc cctcggggag gtcctctgtg
     3541 tcgaactccg aaccgtcgcc ggagccgtcg tcgcccaggt cggaggagat cgatgcgccg
     3601 tagagcgcct gttcgtaggc ggcgcgttcg gagtcgctca tgccgtcgac gatggcctgg
     3661 ttcggatcct gcgacgcggt gtcgtcgaac gaggccatgc cgggagcgtc ctcgacgggc
     3721 gagccgtcgg cctgcatggt cgtggtgatg ccgaagcccc acttctcggc gtactcccgt
     3781 tgggtgagat cgatctcggg ggcggcggcg aagttgcccg ccgtgaggtt cgggatgtac
     3841 tcgaagccct cggcgtgcat gcactcggcg atggcgcgct cggccttctt gttctgctct
     3901 cgtgccgagg cgttgagctt ctcggtgtcg tcgtagccga agtacttggt cagcggcgac
     3961 gacgaggcgt cgccgtactg gcggttaccg aactcgacga cggtggccga gtcctcgctc
     4021 cccgaggact ccttgccgca gcccgtggcc acgagcagcg caaccgctgg agcgacgagc
     4081 catcgggaca cccggcgccc cgacagatgc atgctgaaat tcatgcggcg aatgtactgc
     4141 tgtccgaccg ggggtcgcgc cgcacggcgc gtggatcagt gtcgtcagac ggcgtcgccg
     4201 gtcagcgcct cgctgatgcc ctgatcgagg gtgttccacg acaacgtcgc gcacttgatg
     4261 cgaacgggaa acttcaccac gccgcgcatc gcctcgaggt cgccgagctt gagttcgccg
     4321 atgtcggcga tgtcgggctc ctcgacgctg tcgtcgagtg cgctctcgtg gatcgacatc
     4381 atcgccttga aggcgcgcat gacctcacgg gcctcggcga tcgtcttgcc gttgaccgcg
     4441 gtcgacatca tcgacgcgga cgactggctg atcgagcagc cttgcccgcc gatgcggatc
     4501 tcctcgatga cgcggtcgtc gccttcgccc tcgccgagtt gcaggtacac gacgatctcg
     4561 tcaccgcaca gcgggttgaa accctcggtg cgggtagcgg gtggtgtgtc gagttcgccg
     4621 cggttccggg gcgaacggta atgatcgatg atgatctcgc ggtacagatc ttcgagaccg
     4681 gccatggggg ctcctacgaa cgggtacggg cttgctggag agtgggaatc agaaggcgaa
     4741 gaaggactcg gccgcttcga gggagtcggc aagagcgtcg acgtcggcct cgtcgttgta
     4801 gatgtacacc gatgctcgcg cagtcgcgcc gactccgaga actttcatga gcggcttggc
     4861 acagtggtgg ccggcgcgca cgcacacccc gtgctcgtcg agcacctggg acaggttatg
     4921 ggggtggacc ccggcgagct ccagcgagaa gaccccgccg cgc
//
LOCUS       DAOSOA010000254         4924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17260_length_4924_cov_7.790511, whole genome shotgun sequence.
ACCESSION   DAOSOA010000254 DAOSOA010000000
VERSION     DAOSOA010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17260_length_4924_cov_7.790511"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKY13_13370"
     CDS             <1..715
                     /locus_tag="PKY13_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT80976.1"
                     /translation="AMIGAAKGYPVTLVMPANVSAERKAIVHAYGATLIESDPLEGSD
                     GAIRLVRQIVADDPQRYYYPDQYSNPANWIAHYETTGPEIWADAPEITHFVAGLGTSG
                     TLLGVGTYLKERNPDVSILAVEPPSGELVEGLRSLEDGYIPPVFEKWGGYELLDGKSI
                     VRPRESLEYTRKLAGIGIFAGISSGAALAGACKVAGRIESGTIVFIVCDYGWKYLSTG
                     AWTVDLDEAAANAEKIIYF"
     gene            982..1329
                     /locus_tag="PKY13_13375"
     CDS             982..1329
                     /locus_tag="PKY13_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80977.1"
                     /translation="MSDQEMVAARAAGEDVLRRRGFFANVDRARTFGGNSLSGVLDVA
                     GVATLIVSAVLVVLLLTHHASGPTREDDWLTAAVVAICGATCGLCLFAMSRLVTYVKA
                     CAVLLAKSSADVE"
     gene            1347..1817
                     /locus_tag="PKY13_13380"
     CDS             1347..1817
                     /locus_tag="PKY13_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HOT80978.1"
                     /translation="MSTLPAIPAALHVDDADCPWVDSLMGFEFKLVQVRISEGLWVMR
                     NRFQPGARIQKHKHTGPVYAFTMSGSWKYEEYDDVNRAGSFLYEPAGSTHTLIVPDTN
                     TEVTDVWFAVWGANLNLDADGNVESVFDAQFILDGYVGLCEAAGLPTPKVLIGE"
     gene            complement(1848..2300)
                     /locus_tag="PKY13_13385"
     CDS             complement(1848..2300)
                     /locus_tag="PKY13_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80979.1"
                     /translation="MTSTVGPTVVAERAGRRNLGFASGLALLALAILSPMLRTEPIET
                     GTQIAIGALILVALGIVGLWVYTIRNPTSLVIADDEIRLEPGGLEPERRRIPRSAGSL
                     RIAKEGSLRSKTLVLSTLDREHRLPLHFMDRQEIVDACYDHGWDFGPD"
     gene            complement(2297..2851)
                     /locus_tag="PKY13_13390"
     CDS             complement(2297..2851)
                     /locus_tag="PKY13_13390"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOT80980.1"
                     /translation="MATTTLSRERIVDEAIALLDDDGPDALSMRRLALRLGVSTMSTY
                     HHIADKEALVEGIADRIMGSLERPAPDASWPDAVRTLAWSFRSLTIEHPSVFRVLLVG
                     RRPNALLRTADDVRALLEQRGFDEQEALLVFRTFIRYLMGSTLVELGGPGGGASRSGK
                     AAAERQFGYGLEAIIAGVQATTDR"
     gene            2974..4095
                     /locus_tag="PKY13_13395"
     CDS             2974..4095
                     /locus_tag="PKY13_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT80981.1"
                     /translation="MTDASTRPDHDVNDVDHILAMYSRRDVDAVAHAVELNADALFTW
                     DYERSRHALSKLYEKAKGAQWNGATDLPWDTDVDQEKVATQLVASSIDRFKQLQSIPG
                     SPFRNFGDKEWLQVGVEMQNWQLSQFLHGEQGALMCTGLITATTPWIDAKYYAATQVV
                     DEARHVEVFARYLDEKLSGTYPLNPNLGGLIDDTISDARWDVTYLGMQIMIEGLALAA
                     FGAIHMVTPDPLLKQLLRYVMSDEARHVAFGVLSLQEYYQGLGAAELRERQEFAFEAA
                     EKLRNRFFMVEVWERMGVDVNDVLRTIIDNPPPAQTDFQHLLFSKIVPNCKKLGILDA
                     GDGWLRQKFTELGIIQYESWTDTSDEYETFALENLPAAG"
     gene            4137..>4924
                     /gene="murI"
                     /locus_tag="PKY13_13400"
     CDS             4137..>4924
                     /gene="murI"
                     /locus_tag="PKY13_13400"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOT80982.1"
                     /translation="MTAGPIAMFDSGFGGLTVARAVIDLLPHEEIVYLGDTARYPYGP
                     RPQAEVREFAHQIATELVEEQGAKLLIVACNTAAAAALSELQEHFDIPVVGVIEPGVR
                     SMAAATRNGRVGVIGTVGTIESGAYQTAVAAEAPWVDLCALSCPGFVEFVERGEFRSH
                     QVHVLAERLLAPAIDAGIDTLLLGCTHYPYLARTIGDVMGRDVVLVSSADETAFEVRR
                     ILDGSDLARSVDLPAPEPRFLTTGDVDWFQVLGSQLLGPELDKA"
BASE COUNT          807 a         1724 c         1605 g          788 t
ORIGIN      
        1 cgccatgatt ggcgcagcga agggctaccc cgtcaccctg gtcatgcccg ccaacgtcag
       61 cgccgagcgc aaggccatcg tccacgccta cggcgcgacc ctcatcgagt ccgacccgct
      121 agaaggctcc gacggcgcga ttcgcctcgt ccgccagatc gtcgccgacg acccgcaacg
      181 ctactactac cccgaccaat acagcaaccc cgccaactgg atcgcccact acgagacgac
      241 cggccccgag atctgggcgg acgcccccga gatcacccac ttcgtggctg gcctcggcac
      301 gtccgggacg ctgctcggtg tgggcaccta cctcaaggag cgcaaccccg acgtgtcgat
      361 cctcgccgtc gagccgcctt ccggtgagct cgtcgaaggg ctccgatccc tcgaagacgg
      421 atacatccct ccggtcttcg agaagtgggg cggctacgag ctgctcgacg gcaagtcgat
      481 cgtccggccc cgcgagtcgc tcgagtacac ccgcaagctc gccggcatcg gcatcttcgc
      541 cggcatctcg tcgggcgccg cgctcgccgg cgcctgcaag gtcgccggcc gcatcgagtc
      601 cggcaccatc gtcttcatcg tgtgcgacta cggctggaag tacctgtcga cgggcgcttg
      661 gacggtcgac ctcgacgaag ccgccgccaa cgccgagaag atcatctact tctagggctc
      721 agcgtgcttc gtcacgccgg tggcttcacc atctcgtcgg cttcgccgac caacgaactg
      781 tcttgatggg cgccggcccg acttcgtcga ccgtcgccgg gtccgggttg cgagatcgtc
      841 gtgatgggca ccggcccgac ttcgtcgacc gtcgcgggcc cgacttcgtc gaccgtcgcg
      901 cgtttctgat ccatttcgac tactgccagt cgcactcatg tcaggtacct tgactctcag
      961 cccgcgctgg agggggtgga catgagcgat caggagatgg tggccgctcg agcggcaggt
     1021 gaggacgtgt tgaggcgccg cggcttcttc gcgaacgtcg accgggcccg gacgttcggt
     1081 ggtaactccc tctcgggcgt cctcgacgtc gccggggttg caacgctcat cgtgtcggcc
     1141 gtcctcgtcg tactgctgct cacccaccat gcctcgggtc cgacccgaga ggacgactgg
     1201 ctgacggcgg cggtcgtcgc gatctgcggc gcgacatgtg gcctgtgcct gttcgcgatg
     1261 tcgcgtctgg tcacctacgt gaaggcctgc gcggtgctgc tcgccaagtc gagcgccgac
     1321 gtcgagtagc cacctaccgt gtggccatga gcacgctgcc cgccatcccc gccgccctcc
     1381 acgtcgacga cgccgactgc ccctgggtcg actccttgat ggggttcgag ttcaagctcg
     1441 tccaggtccg catctccgag ggcctctggg tcatgcgcaa ccggttccag cccggcgccc
     1501 gcatccagaa gcacaagcac accggtcccg tctacgcgtt cacgatgtcg ggttcgtgga
     1561 agtacgagga gtacgacgac gtgaaccgcg ccggatcctt cctctacgag ccggcgggct
     1621 ccacccacac gctgatcgtt cccgacacca acaccgaggt gaccgacgtg tggttcgccg
     1681 tctggggtgc caacctcaac ctcgatgccg acggcaatgt cgagagcgtg ttcgacgccc
     1741 agttcatcct cgacggctac gtcggtctct gcgaggcggc cgggctcccg acgcccaagg
     1801 tgctcatcgg cgagtgacgt gccggcggtg acgaccggcg tcggcgctca gtcggggccg
     1861 aaatcccacc cgtggtcgta gcaggcgtcg acgatctcct gccggtccat gaagtggagc
     1921 ggcagccgat gctcgcggtc gagcgtcgac aggacgaggg tcttcgagcg gagggacccc
     1981 tccttggcga tgcgaaggct tcccgccgag cgaggaatcc ggcgtcgctc gggttcgagg
     2041 ccgcccggct cgagtcggat ctcgtcgtcg gcgatgacga gcgacgtcgg attgcggatc
     2101 gtgtagaccc acaacccgac gatgccgagg gccacgagga tcagcgcgcc gatggcgatc
     2161 tgggtgccgg tctcgatggg ctcggtccgg agcatgggcg agaggatcgc gagggcgagg
     2221 agcgcgagtc cggaggcgaa cccgaggttg cggcgtccgg cacgttcggc gacgaccgtc
     2281 ggccccactg tcgaggtcat cggtcggtcg tcgcctggac gccggcgatg atggcctcga
     2341 ggccgtagcc gaactgacgc tccgcggcgg ccttgccgct gcggctggca ccgccgccgg
     2401 gtccgcccag ctccaccagc gtcgaaccca tgaggtaccg gatgaacgtg cggaagacga
     2461 gcagcgcttc ctgctcgtcg aagccccgct gttcgagcag cgcccgcacg tcgtcggcgg
     2521 tccgcagcag ggcgttggga cgccggccga cgaggaggac gcggaacacc gacgggtgtt
     2581 cgatggtgag cgagcgaaac gaccacgcca gcgtgcggac cgcgtcgggc cacgaggcgt
     2641 cgggagcggg tcgctcgagt gaacccatga tccggtcggc gatgccctcg accagcgcct
     2701 ccttgtcggc gatgtggtgg taggtcgaca tcgtcgacac gccgagtcgg agcgcgaggc
     2761 ggcgcatgct cagcgcgtcg gggccgtcgt cgtcgagcag ggcgattgcc tcgtcgacga
     2821 tgcgctcccg ggacagggtg gtggttgcca tgacgtcgca atcgtacgcc gtacgagcta
     2881 aagtcatccg aaaccgtaca ccgtacgaaa cccactcacg ccacccgtgt ccgtctccgg
     2941 cttcgtcgga ggcgccttcg cccaggggat tccatgaccg acgccagcac ccgtcccgac
     3001 cacgacgtca acgacgtcga ccacatcctc gccatgtaca gccggcgcga cgtcgacgcc
     3061 gtggcgcacg cggtcgagct caacgccgac gcgctgttca cgtgggacta cgaacgcagc
     3121 cgccacgcgc tgtccaagct gtacgagaag gcgaagggcg cgcagtggaa cggtgccacc
     3181 gatctcccct gggacaccga cgtcgaccag gagaaggtcg ccacgcagct cgtcgccagc
     3241 agcatcgacc ggttcaagca gctgcagagc atccccggct caccgttcag gaacttcggc
     3301 gacaaggagt ggttgcaggt cggtgtcgaa atgcagaact ggcaactcag ccagttcctg
     3361 cacggtgagc agggtgcgct catgtgcacc ggtctcatca cggcgaccac gccgtggatc
     3421 gacgccaagt actacgccgc cacccaggtg gtcgacgagg ctcgtcacgt cgaggtgttc
     3481 gcccgctacc tcgacgagaa actgagcggc acctacccgc tcaaccccaa cctcggtggt
     3541 ctgatcgacg acacgatcag cgacgcccgc tgggacgtca cctacctcgg catgcagatc
     3601 atgatcgagg ggctcgcgct ggcggccttc ggcgccatcc acatggtcac gcccgacccg
     3661 ctgctcaagc agctgctccg ctacgtgatg agcgacgaag cccgtcacgt ggcgttcggt
     3721 gtgctcagcc tgcaggagta ctaccagggg ctcggagccg ccgagctgcg cgagcgtcag
     3781 gagttcgcct tcgaggcggc cgagaagctc cgcaaccggt tcttcatggt cgaggtgtgg
     3841 gagcgaatgg gtgtcgacgt caacgacgtc ctccgcacga tcatcgacaa tccgcctccg
     3901 gcccagaccg acttccaaca cctcctcttc tcgaagatcg tccccaactg caagaagctc
     3961 ggcatcctcg atgccggaga cgggtggctc cgccagaagt tcaccgagct cggcatcatc
     4021 cagtacgaga gctggaccga cacctccgac gagtacgaga ccttcgcgtt ggagaacctt
     4081 cccgccgctg gctagccagc ggtcgacgga ccccggcggc gctagcgttg ccggcgatga
     4141 ccgccggccc cattgccatg ttcgactccg ggttcggcgg gctcacggtc gcacgagcag
     4201 tcatcgacct gctcccgcac gaggagatcg tctacctcgg cgacaccgcc aggtatccct
     4261 acggtcctcg accccaggcc gaggtgcgcg agttcgccca ccagatcgcc accgagctcg
     4321 tcgaggagca gggcgccaag ctgctgatcg tggcgtgcaa cacggcggca gccgccgcgc
     4381 tgagtgagct gcaggagcac ttcgacatcc cggtcgtcgg cgtgatcgaa ccaggcgtgc
     4441 ggtcgatggc ggcggcaacc cgtaacggac gcgtcggcgt gatcggcacc gtcggcacga
     4501 tcgagtcggg cgcgtaccag acggccgtcg ccgccgaggc cccatgggtc gacctgtgtg
     4561 cgctgagctg cccgggcttc gtcgagttcg tcgagcgcgg cgagttccgc agccaccagg
     4621 tccacgtgct ggccgagcgg ctgctggcac ccgccatcga cgccggcatc gacaccctgc
     4681 tcctcgggtg cacgcactac ccgtacctgg cgcgcaccat cggcgacgtc atgggccgcg
     4741 acgtcgtgtt ggtgtcgagc gcggacgaga ccgcgttcga ggtgcgccga atcctcgacg
     4801 gctccgatct cgcccggtcc gtcgaccttc cggcacctga gccccggttc ctgaccaccg
     4861 gcgacgtcga ctggttccag gtgctcggca gtcagctgct cggccccgaa ctcgacaagg
     4921 ccga
//
LOCUS       DAOSOA010000255         4919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17281_length_4919_cov_6.826275, whole genome shotgun sequence.
ACCESSION   DAOSOA010000255 DAOSOA010000000
VERSION     DAOSOA010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17281_length_4919_cov_6.826275"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PKY13_13405"
     CDS             <1..1322
                     /locus_tag="PKY13_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03767 family metallophosphoesterase"
                     /protein_id="HOT80983.1"
                     /translation="RPATDRYATELGFPTIDGFLPAAIAGFTSVGLDIPWYSTYGNHD
                     GLLQGNLARTDAIDRLLTGDLKITGVGSTGITSLIAAFAADTGSIPERVARGELDGRS
                     VTADPTRRTVTTAEWVAAHLAEPKADSPDRPGPIGHGYDEDHLDGDALYYRFDVSPAV
                     VGLSLDTGGYNSGSISEFQLLWIERQLRSVSSLWYDTDGNERCEACDDRLVVIFSHFT
                     SGTMTGSADPTRPDERRVQGEELVATLLRYPNVIAWVNGHTHSNSIAPMPDPLRRSGG
                     FWQITTASHVDYPEQARIVEVVDNGDDTLSIFTTMIEHAAPATADYGDLSATGLAAIS
                     RELSANNSVGDITRHVGGIDSLNCELGLAAPFPLKSLHPEPERDHQASTTSASVSLSP
                     LMLGGAGAAAAIGGAIAIRRRRAGAPDGVEPDQVQPDERQASGESLS"
     gene            complement(1295..1867)
                     /gene="pyrE"
                     /locus_tag="PKY13_13410"
     CDS             complement(1295..1867)
                     /gene="pyrE"
                     /locus_tag="PKY13_13410"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT80984.1"
                     /translation="MTDESAKGLLIAHLLEHSIKHGDFTLKSGKKSPYFLDSKQTACD
                     PDGVVLMTDVALEHIPSDATAIGGITVGADPVAYGIAAVAATHGRRLRSFTIRKEAKD
                     HGVVGRVAGALRPGDKVVVTEDTTSRGVTLADAIDVVREFGAEPVLVLALVDRGGTAA
                     GYAEAAGVPFVALVTAPDLGFPYDKDSPEA"
     gene            complement(1881..2936)
                     /gene="purM"
                     /locus_tag="PKY13_13415"
     CDS             complement(1881..2936)
                     /gene="purM"
                     /locus_tag="PKY13_13415"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOT80985.1"
                     /translation="MSAASPSPGGATYAAAGVSIDAGEAAVDRIKSQVRSTFRPEVLG
                     DIGGFGGLFALGSRFKDPVLVSTTDGVGTKAMVATAAGKFDTIGLDLVAMCVDDLVCQ
                     GAEPLFFLDYISIGKLDPDHVSELVAGVAEGCRRSGCALIGGEMAEHPGAMEPGEFDL
                     VGFAVGVVERSAIIDGSRVTPGDVLLGLASPGLRSNGYSLARHVLFDIAGRSLGDPAW
                     DDSGAPTVAEELLRPSVIYAPAIAALTSEVEVHSVAHITGGGIGGNLNRVLSPVTDAV
                     VDRGSWEMPRIFAEIQRLGEISDDEMAKVFNLGVGMIVAVPAADAKRAIDVLAAVGQR
                     ATVVGEIVDGSGQVHLT"
     gene            complement(2933..4480)
                     /gene="purF"
                     /locus_tag="PKY13_13420"
     CDS             complement(2933..4480)
                     /gene="purF"
                     /locus_tag="PKY13_13420"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT80986.1"
                     /translation="MTGPALSPGDLTDRDLADDGRPGEECGVFGVYAPGKSVGHLCYL
                     GIFALQHRGQESAGIATSDGKHLTVVKDMGLVSSVFDDRTLAALDGQMGIGHTRYSTT
                     GSSAWQNSQPVYRDVSHTQFALAHNGNLTNTAALAEEAGMLPGMANSDTEVMAELLSQ
                     HLHSNPQCVGHELEGALMGVLPSMQGAFSLVITDAHHIIGVRDPNGFRPLCLGRLDGG
                     WVLASESPALDVVGAHFVRELDPGEMVIIDQDGVRSVRPFPEERVDPKLCLFEFVYFA
                     RPDSTLYGRNVHLARVRMGEQLAEQAPVDADLVMGVPESGLPAAEGYARHSGIPYGHG
                     LVKNRYIGRTFIAPTQDMRAMAVRMKLNPLRENIAGKRLIVVDDSIVRGTTTRAMVQM
                     LREAGAVEVHMRVSSPPYRWPCFYGMDTGTRGELLAANMEVHEIRDYLGVDTLSYLGL
                     DRLIAATGAPTSGYCTACLTGDYPVEIPVELSGSLALPPRADAGVRTAPQLPLDVPPV
                     VAGGGGR"
     gene            complement(4528..>4919)
                     /locus_tag="PKY13_13425"
     CDS             complement(4528..>4919)
                     /locus_tag="PKY13_13425"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOT80987.1"
                     /translation="AAAAVAGLVRQLVVDDRVVGAHDVSSGGIALALAEMAVRSGFGV
                     HCARIADHVELFGESVGRAVLCVEAASAREVIDLCEAHGVPVTQLGAVMGDRIVVKDL
                     LDIDLAEATTTWRERLPRALGAGTVQA"
BASE COUNT          810 a         1730 c         1693 g          686 t
ORIGIN      
        1 accgacccgc gaccgatcgc tacgccaccg aactcgggtt ccccacgatc gacggattcc
       61 tcccggcagc gatcgccggg ttcacttcgg taggtctcga catcccgtgg tactccacct
      121 atggcaacca cgacggactc ctgcagggca acctggctcg caccgacgcc atcgatcgcc
      181 tgctcacggg ggacctcaag atcaccgggg tcggctccac cggcatcacc agcctgatcg
      241 cagccttcgc cgccgacacc ggctcgatcc ccgagcgggt cgcgcgcgga gagttggacg
      301 gccgatcggt cacggccgat ccgacccgcc gcaccgtcac caccgccgaa tgggtcgcag
      361 cgcacctcgc cgaaccgaag gccgattcac ccgaccggcc ggggccgatc ggccacgggt
      421 acgacgagga ccatctggac ggcgacgcgc tctactaccg gttcgacgtg tcacctgcgg
      481 tggtgggcct gtccctcgac accggcggct acaactccgg ttccatcagc gagttccaac
      541 tcctgtggat cgagcggcaa ctgcggtcgg tgagttccct ctggtacgac accgacggca
      601 acgaacggtg cgaggcgtgc gacgaccggc tcgttgtgat cttcagccac ttcaccagcg
      661 gcacgatgac cggctcggcc gatccgaccc gtcccgacga acggcgcgtc cagggcgaag
      721 agctcgtcgc gacactgctc cgctacccca acgtgatcgc ctgggtgaac gggcacaccc
      781 acagcaattc catcgccccg atgcccgacc ccctccggcg ctccggcggc ttctggcaga
      841 tcaccacggc gtcccacgtc gactaccccg agcaggcccg gatcgtggag gtggtcgaca
      901 acggcgacga cacgctctcg atcttcacga ccatgatcga gcacgccgct cccgccaccg
      961 ccgactacgg cgacctgtcg gcgacgggcc tcgccgcgat cagccgtgaa ctctcggcga
     1021 acaacagcgt cggcgacatc acccgccacg tcggcggcat cgactcgctg aactgcgagc
     1081 tcggcctggc cgcgccgttc ccgttgaagt cactccatcc cgaaccggaa cgggaccacc
     1141 aggcctcgac gacctcggcg tcggtcagcc tctccccgct gatgctggga ggcgctggtg
     1201 cagccgctgc catcggcgga gcgatcgcga tccgtcgccg acgcgccggc gcacccgacg
     1261 gggttgaacc cgaccaggtg cagcccgacg agcgtcaggc ctccggcgag tccttgtcgt
     1321 aggggaaccc gagatcgggc gccgtcacca gcgccacgaa cggcaccccg gccgcctcgg
     1381 cgtagcccgc cgccgtgccg ccacgatcga ccagggccag cacgaggacc ggctcagcgc
     1441 cgaactcgcg cacgacgtcg atcgcgtcgg cgagcgtcac gccccgcgag gtcgtgtcct
     1501 cggtgacgac caccttgtcg cccggacgga gcgctccggc gacgcgaccc acgacgccgt
     1561 ggtccttggc ttccttgcga atcgtgaagg aacgaaggcg tcggccgtgg gtcgccgcca
     1621 ccgcggcgat gccgtacgca accggatccg caccgacggt gatgccgccg atggcagtcg
     1681 cgtcactcgg gatgtgctcg agcgcgacgt cggtcatgag gacgaccccg tcggggtcgc
     1741 acgccgtctg cttcgaatcg aggaagtacg gcgacttctt gcccgacttc agcgtgaagt
     1801 cgccgtgctt gatcgaatgc tcgaggaggt gggcgatcaa caagcccttc gctgactcgt
     1861 cggtcatgcc ggggtcgtac tcacgtgagg tggacctggc cggaaccgtc gacgatctcg
     1921 ccgaccacgg tggcgcgctg tccgaccgcg gcgaggacgt cgatggcacg cttcgcgtcg
     1981 gccgccggga cggccacgat catgccgaca ccgaggttga acaccttggc catctcgtcg
     2041 tcgctgatct cgcccagccg ctggatctcg gcgaagatgc gcggcatctc ccacgagccc
     2101 cggtcgacga ccgcgtcggt caccggcgac agcacgcggt tgaggttgcc gccgatgccc
     2161 ccgccggtga tgtgcgcaac ggagtgcacc tcgacctccg acgtgagggc tgcgatcgcc
     2221 ggtgcgtaga tgaccgacgg acgcagcagc tcctcggcga cggtcggcgc gccggagtcg
     2281 tcccacgccg ggtcacccag cgaccgtccg gcgatgtcga acaacacgtg gcgggcgagc
     2341 gagtagccgt tggaacggag accgggcgaa gcgagtccga ggagaacgtc gcccggcgtg
     2401 acccgcgaac cgtcgatgat cgcgctgcgc tcgacgacgc cgacggcgaa gccgacgagg
     2461 tcgaactcac cgggctccat ggcaccgggg tgctcggcca tctcgccgcc gatcagcgcg
     2521 cagccggatc ggcggcaccc ctcggcgaca ccggcgacca gctccgacac gtggtcgggg
     2581 tcgagcttgc cgatgctgat gtagtcgagg aagaacagcg gttcggcgcc ctggcacacg
     2641 agatcgtcga cacacatcgc gacgaggtcg aggccgatcg tgtcgaactt gcctgctgcc
     2701 gtcgcgacca tggccttggt gcccacgccg tcggtggtgg acacgagcac cgggtccttg
     2761 aagcgggaac ccagggcgaa caggccaccg aagccgccga tgtcgccgag cacctcgggg
     2821 cggaacgtgg atcggacctg ggacttgatg cggtcgacgg cggcctcacc ggcgtcgatc
     2881 gagaccccgg cggcggcgta ggtggcacca cccggggagg gagatgccgc gctcacctgc
     2941 cgccaccccc ggcgacgacc ggtgggacgt cgagcgggag ctggggcgcg gtgcgcacgc
     3001 cggcatcggc cctcggcggg agggccagcg accccgacag ctcgacgggg atctcgacgg
     3061 gatagtcacc ggtcaggcaa gcggtgcagt accccgacgt cggcgcgccg gtcgcggcga
     3121 tgagacggtc gaggccgagg taggacaacg tgtcgacgcc gaggtagtcg cggatctcgt
     3181 gaacctccat attggcggcc agcaactccc cacgggtccc cgtgtccatg ccgtagaagc
     3241 agggccagcg gtacggcggt gacgacaccc gcatgtggac ctcgacggcg ccggcctccc
     3301 gaagcatctg caccatcgcc cgggtggtcg tgccgcgcac gatcgagtcg tcgacgacga
     3361 tcagccgctt gccggcgatg ttctcccgca gcgggttgag cttcatgcgg accgccatcg
     3421 cccgcatgtc ctgcgtgggg gcgatgaagg tgcgaccgat gtagcggttc ttcacgaggc
     3481 cgtggccgta ggggatgccg ctgtgacggg cgtacccctc tgccgccggc aggcccgact
     3541 ccggaacacc catgacgagg tcggcgtcga cgggggcctg ttccgccagc tgctcgccca
     3601 ttcggacgcg agccaggtgg acgttgcgcc cgtagagggt cgagtcgggt cgggcgaagt
     3661 agacgaactc gaagaggcag agcttggggt cgacgcgctc ctcggggaac ggccggaccg
     3721 accgcacgcc gtcctggtcg atgatgacca tctcgccggg gtcgagctcg cgaacgaagt
     3781 gggcaccgac gacgtcgagg gccggcgact ccgacgcgag gacccaaccg ccgtcgaggc
     3841 ggccgaggca gagcgggcgg aagccgttgg ggtcgcgcac gccgatgatg tggtgggcgt
     3901 cggtgatcac gagggagaag gcaccctgca tgctcggcag gacacccatg agcgcaccct
     3961 cgagttcgtg accgacacac tgcgggttgc tgtgcaggtg ctgggacagc aactccgcca
     4021 tgacctcggt gtcggagttc gccatgcccg gcaacatgcc ggcttcctcg gcgagggccg
     4081 ccgtgttcgt gaggttgccg ttgtgggcga gggcgaactg ggtgtgcgac acgtcgcgat
     4141 agaccggctg gctgttctgc cacgccgacg agccggtggt ggagtaccgg gtgtgcccga
     4201 tgcccatctg cccgtcgagc gccgcgaggg tgcgatcgtc gaagaccgac gacacgagac
     4261 ccatgtcctt gaccacggtc aggtgcttgc cgtcgctcgt ggcgatgccg gccgactcct
     4321 gtccacgatg ttggagggcg aagatgccca gatagcagag gtgtccgacc gacttgcccg
     4381 gggcgtagac gccgaagacg ccacattcct ctccgggacg accgtcgtcg gcgagatccc
     4441 ggtccgtgag gtcaccggga gatagcgctg ggcccgtcac ggtcaccatc gttccacacc
     4501 gggagggccg gacgcgaacc agcgggttca ggcctggacc gtgccggcgc ccaaggcgcg
     4561 gggaaggcgc tcacgccagg tggtggtcgc ctcggcgagg tcgatgtcga gcaggtcctt
     4621 gaccacgatg cggtcaccca tgaccgcgcc gagctgggtc acggggacgc cgtgggcctc
     4681 gcacaggtcg atgacctcgc gggccgacgc cgcctcgaca cacaggacgg cccgaccgac
     4741 cgactccccg aagagctcga cgtggtcggc gatccgggcg cagtgcacac cgaacccgga
     4801 acgcaccgcc atctcggcca gcgccagcgc gatgcccccc gacgagacgt cgtgggcccc
     4861 gacaacacgg tcgtcgacga cgagctgccg tacgaggccg gccacggccg ccgcggcgt
//
LOCUS       DAOSOA010000256         4895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17394_length_4895_cov_5.825620, whole genome shotgun sequence.
ACCESSION   DAOSOA010000256 DAOSOA010000000
VERSION     DAOSOA010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17394_length_4895_cov_5.825620"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..983)
                     /gene="glpX"
                     /locus_tag="PKY13_13430"
     CDS             complement(15..983)
                     /gene="glpX"
                     /locus_tag="PKY13_13430"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOT80988.1"
                     /translation="MTDMPTPDRNLAMELVRVTEAAALAAARWVGGGDKLKADGAAVD
                     AMRHMLTSVAMKGTVIIGEGEKDEAPMLFNGEVVGDLTGFECDIAVDPIDGTTLTALG
                     RGNAVAVIAVSERGTMYDPGPCVYMDKIAVGPDCKGVIDINKSVADNLAAVAAAKGSK
                     VRDLTAVILDRERHSDLIAEVRAAGARIRLIPDGDVAGAISTAWPDSGADIMFGIGGT
                     PEGVIAAAALKCMGGEIQGKLWPRNDEERNAALEAGYDLDQVLTTDVLVAGDNCFFSA
                     TGITDGELIKGVHYSKLGATTQSLVMRSKSGTVRVVEAHHAPDKFI"
     assembly_gap    1127..1226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1227..2075
                     /locus_tag="PKY13_13435"
     CDS             <1227..2075
                     /locus_tag="PKY13_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80989.1"
                     /translation="DLQPSDEPESDDHLDETEAEDTPEVDADDDDEHDLGDDDDDIVD
                     AEMVEVDATEFDDHVDYEIAEWSGQSRALLDSMLETEGIDHIWQGTTLSVRVADEAVT
                     DSLIEIVTQTASQGLDPDRARVVYEVGTWSAAMQTSLAEALGIAEVAFEWDENGDLVI
                     YEDDEEEVEGILDAMPDPDDPDAVDTDGVDVQDVLSKLWGAVGTLAKRPTDSDAVLEA
                     IDAANQIEHIGLPFGFESAIWRDIVGKTLALRAALDSDNEDDQLSDDELQDGCADLHR
                     QLRAYI"
     gene            2195..3526
                     /locus_tag="PKY13_13440"
     CDS             2195..3526
                     /locus_tag="PKY13_13440"
                     /inference="COORDINATES: protein motif:HMM:NF037690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT80990.1"
                     /translation="MVSSAVRSHATRRGRHRALGAGVALGVVTLVGGSALVSAAPTAT
                     VPGAITSVDIAATTSGPFEKVRIDLRFAVPDQAVAGDEFHLALPAQLMAAGPGFEVKD
                     AAGQPIAVASTVGNVVTFTTTAYVDQHDAVVGSAFLEAGWDRRKVTAGDTVELVFDAD
                     GSTFRDSVTLSGGLPDRSRPDAWLGWADERAEDSKGPTNALRWVVASPQTLAGSSGTV
                     SVVVTPGAGSSLACDRVTVAESTKLDEFHEFVGQTSVPAARRTITCGAGQIAVDVTGV
                     AASTWVLVVGRATVTDPALGEYTNSATVTANGATATARGLAKHSPAGGNGGGAARPAT
                     AGGSTKQPPTAGGSGSARIDSDPTESGSGSAALSAGDGLDTGGVDAAQAGAGSGVGSA
                     IGSGETGEPASIGTSNTGVDADRSNGFQGRALVFLAVVAIGIGFVWRRRRH"
     gene            3604..4302
                     /locus_tag="PKY13_13445"
     CDS             3604..4302
                     /locus_tag="PKY13_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80991.1"
                     /translation="MTLAIFEAYYSRPIAPTRRVALGKMMLPVDPAPGFGGVLLGGIM
                     ARFVRELDADLDEELDALVDDLEVGRHIGQPRLRHRLQVDRVGLARCRHRLEADGEQF
                     RFEFESNIGTPTQHVLCAAYAASQVVTEERASLFTAIRKGLDWVGPIDDALVRYLSDR
                     RTLGASAGSDPKGWALERLGIAGSLSGEALHAEVSRSFRELLIQAHPDHGGSVELAAD
                     RIAELREARRILIP"
     gene            4299..4880
                     /locus_tag="PKY13_13450"
     CDS             4299..4880
                     /locus_tag="PKY13_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOT80992.1"
                     /translation="MTARRAPAVAGVILTHGAGSDKDHTMLLAIEERLAPLPVVRMNF
                     PYRDKPGKRPPDRAPVLLAALHAELDALCERLGVSSDRIVLGGRSMGGRMCTMVAAEP
                     TPLAGVIPISYPLHPPGKPEKLRVEHLPDIVAPCLFISGEKDPFGSPDELRAATATIP
                     GPVEHHWVPGGHDFKKSDAEVCDVIHDWIGRLG"
BASE COUNT          729 a         1674 c         1659 g          733 t
ORIGIN      
        1 gcctccggcc ggaatcagat gaacttgtcg ggggcgtggt gcgcttcgac cacgcggacc
       61 gtgcccgact tcgaacgcat cacgagcgac tgcgtggtgg cgccgagctt cgagtagtgc
      121 acacccttga tgagctcgcc gtcggtgatg ccggtggcgc tgaagaagca gttgtcgccg
      181 gcgaccagca cgtcggtggt gaggacctgg tcgaggtcgt atcccgcctc gagcgcagcg
      241 ttgcgctcct cgtcgttgcg gggccagagc ttgccctgga tctcgccgcc catgcacttg
      301 agcgcggcgg cggcgatgac accctcaggg gtgccgccga tgccgaacat gatgtcggct
      361 cccgagtcgg gccacgccgt cgagatggcg ccggcgacgt cgccgtcggg gatgaggcgg
      421 atacgggcgc cggcggcacg gacctcggcg atgaggtcgc tgtgacgctc gcggtccaag
      481 atgacggcgg tgaggtctcg caccttgctg cccttggcag cggcgacagc agcgaggttg
      541 tcggcgaccg acttgttgat gtcgatgaca cccttgcagt cggggccgac ggcgatcttg
      601 tccatgtaga cgcaggggcc cgggtcgtac atcgtgcccc gctcgctgac ggcgatgacg
      661 gcaacggcgt tgccccggcc gagcgccgtg agcgtggtgc cgtcgatcgg gtcgacagcg
      721 atgtcgcact cgaagccggt gaggtcgccg acgacctcac cgttgaacag catcggggct
      781 tcgtccttct cgccctcgcc gatgatgacg gtgcccttca tggccaccga ggtgagcatg
      841 tggcgcatgg cgtcgacggc cgcgccgtcg gccttgagct tgtcgccacc gcccacccac
      901 cgggcggcgg cgagcgctgc ggcctcggtc acgcggacca gttccatggc gaggttgcga
      961 tcgggcgtcg gcatgtcggt catgggggaa acgttaccga gaagggttgg cgttctccga
     1021 atcgtggccc cgctcggcga ggtgccatct gcaaggatgg ccggcgtgaa ggatctccag
     1081 cccagcgacg agcccgaatc cgacgaccac ctcgacgaga ccgaggnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnngatc tccagcccag cgacgagccc gaatccgacg
     1261 accacctcga cgagaccgag gccgaggaca cgcctgaggt cgatgccgac gatgacgacg
     1321 agcatgacct cggtgatgac gacgacgaca tcgtggacgc cgagatggtc gaggtcgacg
     1381 cgaccgagtt cgacgatcac gtcgactacg agatcgcgga gtggtccggg caatcccgtg
     1441 cgctgctcga ctccatgctc gagaccgagg gcatcgatca catctggcag ggcaccacgc
     1501 tgtcggtccg tgtcgccgac gaggcggtga ccgactcgct gatcgagatc gtgacgcaga
     1561 ccgcgagcca ggggctcgac cccgatcgag ctcgcgtggt ctacgaggtg ggcacctggt
     1621 cggctgcgat gcagacgtcg ctcgcggagg cgctcggcat cgccgaggtt gcgttcgaat
     1681 gggacgagaa cggcgatctg gtgatctacg aggacgacga ggaggaggtc gagggaatcc
     1741 tcgacgccat gcctgacccc gacgatcccg acgcggtgga caccgacggc gtcgacgtcc
     1801 aggacgtgtt gtcgaagctg tggggcgccg tcggcacgct ggcgaaacgc ccgaccgact
     1861 ccgatgcggt gctggaggcc atcgacgccg ccaatcagat cgagcacatc ggcctgccgt
     1921 tcgggttcga gtcggccatc tggcgtgaca tcgtcggcaa gacccttgcc ctccgcgccg
     1981 cgctcgacag cgacaacgag gacgatcaac tgtccgacga cgagctgcag gacggctgcg
     2041 ccgatctgca ccgccagctc cgcgcctaca tctgaaccga cacctcggcc ggtagccccg
     2101 ccgaggtgga aatccggcaa gaagtaccta gccttacctc gacacatctg ccaccgtctc
     2161 atccaggttg gcctgtgttg aaaggcgttc atccatggtg tcatcggctg tccgatccca
     2221 cgcgacacgg cgtgggcgtc accgcgccct cggtgcgggc gtcgcgctcg gcgtcgtcac
     2281 gttggtcggc ggatccgcgc tcgtcagtgc agcgccgacg gccacggtgc ccggtgccat
     2341 cacgtccgtc gacatcgcgg ccacgacgtc gggacccttc gaaaaggtcc ggatcgacct
     2401 tcggttcgcc gtgcccgacc aggccgtcgc tggcgacgag ttccacctgg cgttaccagc
     2461 ccagctcatg gccgccggtc cggggttcga ggtcaaggac gctgccggtc aaccgatcgc
     2521 cgtcgccagc acggtcggca acgtcgtcac gttcacgacg accgcgtacg tcgaccaaca
     2581 cgacgccgtc gttggctcgg cgttcctcga ggccggctgg gaccggcgca aggtcaccgc
     2641 cggggacacc gtcgagctgg tgttcgacgc cgatgggtcg acattccgtg actcggtcac
     2701 gctgtctggc gggcttcccg accgctcccg tcccgacgca tggctcggct gggccgacga
     2761 acgcgccgag gattccaagg gcccgaccaa cgcgctccgc tgggtcgtcg cgtcgcccca
     2821 gaccctggcg ggcagctcgg ggaccgtctc cgtcgtggtc acacccggtg ccgggtccag
     2881 cctcgcctgc gaccgggtca cggtcgccga gtcgaccaaa ctcgacgagt tccacgagtt
     2941 cgtcggccag acgtcggtgc cggcggctcg ccgcactatc acctgcgggg ccggacagat
     3001 cgccgtggac gtgaccggcg tcgcggcgtc cacctgggtg ctcgtggtgg gacgcgcgac
     3061 cgtcaccgac ccggcgctcg gcgagtacac gaactcggcc acggtcactg cgaacggggc
     3121 aaccgcgacc gctcgcggcc tcgcgaagca cagcccggcg ggcggcaacg gcggtggggc
     3181 ggcccggccg gcgaccgccg ggggatcgac caagcaacct cccaccgccg gtggcagcgg
     3241 gtcggcacgg atcgattcgg accccaccga atccggctcg gggtcggcgg ccctgtcggc
     3301 cggagatggc ctcgacacgg gcggggtgga cgcggcgcag gcaggcgcgg gaagtggcgt
     3361 cggttcggcc atcgggtccg gcgagaccgg cgagccggcg tcgatcggga cgtccaacac
     3421 cggcgtcgat gcggaccggt cgaacggctt ccaggggcgg gcgctggtgt tcctggccgt
     3481 cgtggccatc ggcatcgggt tcgtctggcg ccggcgtcgt cattgacgat ctcgtcgggc
     3541 gtgtcgaccg ccacaccggc aggggccttg ggaccgcagc gccgggcgca gtagcctccc
     3601 cacatgacgc tggcgatctt cgaggcgtat tactcccgcc cgatcgcccc gacccgccgg
     3661 gtggcgctcg gcaagatgat gctcccggtc gaccctgcgc cgggcttcgg cggtgtgctg
     3721 ttgggcggca tcatggcgcg gttcgtccgc gagctcgacg ccgatctcga cgaggaactc
     3781 gacgcgctcg tcgacgatct cgaggtcggc cgccacatcg gccaaccccg gctccgccac
     3841 cggctgcagg tcgaccgggt cgggctggcc cgatgccgtc atcgactcga ggccgacggc
     3901 gagcagttcc ggttcgagtt cgaatcgaac atcggcacgc cgacccaaca cgtgctctgc
     3961 gccgcctatg ccgcctccca ggtggtgacc gaggagcggg cctcgctgtt caccgcgatc
     4021 cgcaagggcc tcgattgggt cggcccgatc gacgacgcgc tcgtgcgcta cttgtccgac
     4081 cgccggaccc tcggagcgtc cgccggatcc gatcccaagg gctgggcgct cgaacgcctg
     4141 ggcatcgccg ggtcgctcag tggcgaggcg ctccacgcgg aagtgtcgcg gagcttccgt
     4201 gaactcctca tccaggccca ccccgaccac ggcggttcgg tcgaactcgc ggcggaccgc
     4261 atcgccgaac tccgcgaagc ccgacggatc ctgatcccgt gaccgctcgg cgagcaccgg
     4321 ccgtcgccgg ggtgatcctc actcacggcg ccggctccga caaggaccac acgatgctcc
     4381 tcgccatcga ggagcgcctc gcgcccctcc cggtggtccg gatgaacttc ccctaccgcg
     4441 acaagcccgg aaagcgaccg ccggaccgag ctcccgtcct cctcgcggcg ctgcacgccg
     4501 agttggacgc attgtgcgaa cgtctcggcg tgtccagcga tcgcatcgtg ttgggcggcc
     4561 gctcgatggg cggtcggatg tgcacgatgg tcgcggccga gccgaccccg ctcgccggtg
     4621 tgatcccgat ctcgtacccg cttcacccgc ccggcaagcc cgagaagttg cgggtcgagc
     4681 acctccccga catcgtcgct ccctgcctgt tcatctccgg cgagaaggac cccttcggaa
     4741 gccccgacga gctgcgtgcc gccacggcca cgatccccgg gccggtggag caccactggg
     4801 tgcccggcgg gcacgacttc aagaagtccg atgccgaggt ctgcgacgtg atccacgact
     4861 ggatcggccg actcggctga gctgatcgcc cgccc
//
LOCUS       DAOSOA010000257         4890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17413_length_4890_cov_6.733609, whole genome shotgun sequence.
ACCESSION   DAOSOA010000257 DAOSOA010000000
VERSION     DAOSOA010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17413_length_4890_cov_6.733609"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKY13_13455"
     CDS             <1..432
                     /locus_tag="PKY13_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOT80993.1"
                     /translation="GVLDDVTVTQVSTVQALLRANPSMPRRDLYREATHVGRDHIAST
                     VNTLVLAYAGASLPLLLYFAEGTAPVGRLLTRELVAVEIIRMLVGSIGLILSVPVTTG
                     LAAAVLTPAHAAAGHGHGHGPDTGPAPDPAPSWNDFGPIDL"
     gene            459..1262
                     /locus_tag="PKY13_13460"
     CDS             459..1262
                     /locus_tag="PKY13_13460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT80994.1"
                     /translation="MALGSTDNSTMGGDQHQSRFQRGLLRLTLERGARWTIVIVTGAS
                     LTVSLSMTSLSFLIFPADRQTVIVSIALATIIPMIVAPLATHVIVGLVLSVASANDEL
                     QRLATTDSLTGLANRRRFFTAAAGIIARPDPETVTLVGMVDVDSFKLVNDRHGHAAGD
                     AVLATLAERLRRAVGDNTSVVGRLGGDEFGIVLTAPEDALVAVTDALQQACSDIEIGS
                     SDLARATLGLCCPGGDGTNESIDDLLARADAALYAAKLARRLNPPASLV"
     gene            complement(1237..2496)
                     /locus_tag="PKY13_13465"
     CDS             complement(1237..2496)
                     /locus_tag="PKY13_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT80995.1"
                     /translation="MSERLALGDPLFWRRPLTDRMADFAAFRAESPFTHALSDNPLTG
                     VPDEFYAVTTYAELVEISRHPLDYCSGKGSISIADMPIDAQEFFGSFITMDDPRHARL
                     RNIVSRSFTPRALQGVLDSVETICTEVIDNMCEQGEVDLVEAISQPFPLLVICDMMGI
                     PRSEFQTVLDATNTILGAGDPDMMGGQTDIVTALLMAGMTLTQLMGEIAEDRRKNPTD
                     DLTSALVHSSTGEELLTPEEIAPFFILLAVAGNDTTRTATSIGMQLLGEHPDQRRIWQ
                     DDLDGVTPTAVEEIVRMASPVTFMRRTATRDLELSGHSFSEGDKFVLFYGAANRDPLV
                     FDDPERFDVRRDPNPHVGFGGPGPHFCLGAHLARRELSVIFRQLFTRLPDIEVVGEAI
                     PLEAMGVPLVGGIKHLPVRFTPTKRVG"
     gene            complement(2529..3260)
                     /locus_tag="PKY13_13470"
     CDS             complement(2529..3260)
                     /locus_tag="PKY13_13470"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOT80996.1"
                     /translation="MSAVGTETVEQAAVPGSAGWWLERRARDDRRRPRVDGLSIERMI
                     LTAIAVVDDEGLDALTVRRLAADLETGSASLYRHVASRDELLVLMVDHVLGEIVFPDE
                     HLPARARVEGLATELRRVLMAHPHLLPALTASPLLGPNAMRGAERGLADMLEVGFEPA
                     AAVPGYLALIDYVLGTVYFDTSSAGQGTRAASERRELIEALPTEVFPTLRGNEEEFAT
                     PSVEEVFTFGLVTFLDGMESRFPLT"
     gene            complement(3299..>4890)
                     /locus_tag="PKY13_13475"
     CDS             complement(3299..>4890)
                     /locus_tag="PKY13_13475"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT80997.1"
                     /translation="IDATGLLVTPGWVDIHTHYDGQATWDSMLAPSAWHGVTTLVMGN
                     CGVGFAPVVPERRDWLIGLMEGVEDIPGTALAEGIDWSWETFPEYLDALEPRRWTMDI
                     GTQIAHGAVRAYVMGERGARNEPATPDDIEAMREIVSDAVAAGALGFSTSRTIAHVAI
                     DGEPVPGTFAAEDELFGIGSALGDLGAGVFELAPMGAAGEDTVNPIKEVDWMRRLSAK
                     IGRPVTFALIQVDSAPGLWKELMDASLAAADEGADIWPQVAGRATGLLGGHFTTYSFF
                     DVVPAYMDLKLRKLPEAEMVAALRDPAVRASILGWEPDDATRERLEHAAEHTFLLGDP
                     PDYEPSADRSLAGMARASGKDPLEVAYDAMLENDGHGLLYVPILNYSDGWLEPVRQML
                     LHPRSASGLADGGAHCGVICDASLPTFMLTHWTRDRQRGETLPLEWIVKKQTHDTARL
                     YGLGDRGTIEPGMLGDLNVIDYEHLQLGSPYVTADLPAGGKRLLQRATGYRATVKSGV
                     VTFADGEDTGERPGVLLRGAR"
BASE COUNT          730 a         1694 c         1722 g          744 t
ORIGIN      
        1 ggtgtgctcg acgacgtcac ggtgacccag gtgtcgaccg tgcaggcgct gctgcgggcg
       61 aacccgtcga tgccccgccg agacctctac cgcgaagcga cccacgtcgg acgcgaccac
      121 atcgcctcca cggtgaacac cctggtgctg gcgtacgccg gcgcatcgtt gccgctgctg
      181 ttgtacttcg ccgagggcac cgctcccgtc ggccggttgc tcacgcgcga actcgtcgcc
      241 gtcgagatca tccgcatgct cgtcggatcg atcggactca tcttgtcggt ccccgtcacg
      301 acgggcctcg ccgccgccgt gctcaccccg gcgcacgccg cggccgggca cggccacggc
      361 cacggtcccg ataccggccc tgcccccgac cctgcaccca gctggaacga cttcgggccg
      421 atcgacctct gacccgaaga ttccgacgct cgatcaaggt ggcgctcgga tcgaccgaca
      481 actccaccat gggaggcgat cagcaccagt cgaggttcca gcgcgggttg ctccgactca
      541 cgctcgagcg cggcgcccgc tggacgatcg tcatcgtgac aggagcatcg ctgacggtgt
      601 cgttgtcgat gacttccttg tcgttcctga tcttccccgc cgatcgacag acggtcatcg
      661 tgtcgatcgc gctcgcgacg atcatcccga tgatcgtcgc accgctcgcg acccacgtga
      721 tcgtcggcct cgtgctgtcg gtcgcctcgg ccaacgacga actccagcgt ctggccacga
      781 ccgactccct caccggtctc gccaaccggc gacgcttctt caccgcggct gcgggcatca
      841 tcgcccggcc cgatcccgag acggtgacgc tcgtgggaat ggtggacgtc gactccttca
      901 agctcgtcaa cgaccgccac ggccacgccg ccggcgacgc cgtcctcgcc acgctggccg
      961 agcggctgcg ccgagcggtc ggcgacaaca cttcggtcgt ggggcgcctc ggcggcgacg
     1021 agttcggcat cgtgctgacc gcgcccgagg acgcgctcgt tgcggtcacc gacgcactcc
     1081 aacaggcgtg ctccgacatc gagatcgggt cgtcggacct ggcgcgggcg acactcggcc
     1141 tgtgttgccc agggggcgac ggaaccaacg agtcgatcga cgacctgctc gcccgagccg
     1201 acgccgcgct ctacgcggcg aaactcgccc ggcggctcaa cccacccgct tcgttggtgt
     1261 gaaccgcacc ggcaggtgct tgatgccacc gacgagcggc acacccatgg cctcgagcgg
     1321 gatggcctcg ccgacgacct cgatgtcggg caaccgggtg aacagctgac ggaagatgac
     1381 cgagagctcg cgccgggcga ggtgcgcgcc gaggcagaag tgggggccgg ggccgccgaa
     1441 cccgacgtgg gggttgggat cgcgacgcac gtcgaaccgc tcggggtcgt cgaacaccag
     1501 cgggtcgcgg ttggcggcgc cgtagaacag cacgaacttg tcgccctcgg agaacgagtg
     1561 gccggacagc tcgaggtcgc gcgtggccgt gcgccgcatg aaggtgaccg gcgacgccat
     1621 gcgcacgatc tcctcgaccg cggtcggggt cacgccgtcg aggtcgtcct gccagatgcg
     1681 gcgctggtcg gggtgctcgc cgaggagctg catcccgata ctcgtcgccg tgcgagtcgt
     1741 gtcgttgccg gcgacggcca ggaggatgaa gaacggggcg atctcctcgg gggtcaacaa
     1801 ctcctcgccg gtgctgctgt gcacgagcgc cgacgtcagg tcgtcggtgg ggttcttgcg
     1861 ccggtcctcg gcgatctcac ccatgagttg ggtgagcgtc atgcccgcca tgagcagggc
     1921 ggtcacgatg tcggtctggc cgcccatcat gtcggggtcg ccggcgccga ggatggtgtt
     1981 ggtggcatcg agcaccgtct ggaactcgct gcgcgggatg cccatcatgt cgcagatcac
     2041 gaggagcggg aacggctggg agatcgcctc gacgaggtcg acctctccct gctcgcacat
     2101 gttgtcgatg acctcggtgc agatcgtctc gaccgagtcg agcacccctt ggagggcccg
     2161 cggcgtgaac gagcgggaga cgatgttgcg gaggcgggcg tggcggggat cgtccatcgt
     2221 gatgaacgac ccgaagaact cctgcgcgtc gatcggcatg tcggcgatcg agatggagcc
     2281 cttgcccgag cagtagtcga gcgggtgccg gctgatctcg acgagctcgg cgtaggtggt
     2341 gacggcgtag aactcgtcgg gcacgcccgt gagggggttg tcgctcaacg cgtgcgtgaa
     2401 cgggctctcg gcccggaacg ccgcgaagtc ggccatgcgg tcggtcaggg gccgacgcca
     2461 gaagaggggg tcaccaaggg cgagtcgttc ggacatgccg ggcagcgtag gcgatcgccg
     2521 ctcgcatttc aggtcaacgg aaaccgggac tccatgccgt cgaggaacgt caccagcccg
     2581 aaggtgaaga cctcctcgac cgagggcgtc gcgaactcct cctcgttgcc ccgcagggtc
     2641 gggaagacct cggtcgggag ggcctcgatc agttcacgcc gctccgacgc cgcccgggtg
     2701 ccctggccgg cgctgctcgt gtcgaagtac acggtgccga gcacgtaatc gatcagcgcg
     2761 aggtagcccg gcacggcggc ggcgggttcg aagccgacct ccaacatgtc ggcgaggccg
     2821 cgctccgctc cccgcatggc gttgggcccg aggaggggcg atgccgtgag cgccggcaag
     2881 aggtgggggt gcgccatgag cacccgccgc agctcggtgg cgagcccctc cacccgggcg
     2941 cgggccggca gatgctcatc ggggaagacg atctcgccga gcacgtgatc gaccatcagc
     3001 accagcagct cgtcgcggct cgcgacgtgc cggtagagcg acgcgctccc cgtctcgaga
     3061 tcggcggcga ggcgccgcac cgtcaacgcg tcgaggcctt cgtcatcgac gacggcgatt
     3121 gccgtgagga tcatgcgctc gatcgacagg ccatcgacgc ggggccgacg acggtcgtcg
     3181 cgggcgcgtc gttcgagcca ccacccggcg ctgccgggca ccgccgcctg ctcgactgtc
     3241 tccgtcccga ccgcgctcac gggccgacgc tagcgccccg attcggggcg ccgtggcgtc
     3301 agcgggcgcc gcgcagcagg acgccgggac gttcgcccgt gtcctcgccg tcggcgaacg
     3361 tgacaacacc cgacttcacc gtcgcccggt agccggtcgc ccgctggagc aggcgcttgc
     3421 cgccggcagg gagatcggcg gtgacgtagg ggctgccgag ctgcaggtgc tcgtagtcga
     3481 tgacgttgag gtcgccgagc atgcccggct cgatcgtgcc ccgatcaccg aggccgtaca
     3541 gccgagcggt gtcgtgggtc tgcttcttca cgatccactc gagcggcagg gtctcgcccc
     3601 gctgccggtc gcgggtccag tgcgtgagca tgaacgtcgg gagggacgcg tcgcagatca
     3661 cgccgcagtg cgctcctccg tcggcgagac cgctcgcgga ccggggatgg agcagcatct
     3721 gccggaccgg ttcgagccag ccgtcgctgt agttgaggat cggcacgtac agcaggccgt
     3781 ggccgtcgtt ctcgagcatg gcgtcgtacg cgacctccag cggatccttg cccgacgccc
     3841 gggccatccc ggcgagcgag cggtcggcgc tcggctcgta gtcgggcgga tcacccaaca
     3901 ggaaggtgtg ctccgccgcg tgctcgaggc gctcccgggt ggcatcgtcg ggttcccacc
     3961 cgaggatgga tgcccgcacc gccgggtcgc gcagcgctgc gaccatctcg gcctcgggaa
     4021 gcttgcgcag cttcaggtcc atgtacgccg gcacgacatc gaagaacgag tacgtcgtga
     4081 agtgtccgcc gaggaggccg gtcgcacgac cggcgacctg gggccagatg tcggcgccct
     4141 cgtcggccgc ggcgagcgat gcgtccatga gctccttcca gagcccgggg gcggagtcga
     4201 cctggatgag cgcgaacgtg accggtcggc cgatcttcgc ggagaggcga cgcatccagt
     4261 cgacctcctt gatcgggttg acggtgtcct caccggcagc gcccatgggc gccagctcga
     4321 acacgccggc gccgaggtcc ccgagggcgg acccgattcc gaagagctcg tcctcggcgg
     4381 cgaaggtgcc gggcaccggt tcgccgtcga tcgcgacgtg ggcgatggtc cgcgacgtcg
     4441 agaacccgag cgctcccgcc gcaacggcgt cgctcacgat ctcgcgcatg gcctcgatgt
     4501 cgtcgggggt tgccggctcg ttgcgggcac cgcgttcccc catcacgtag gcgcgcacgg
     4561 cgccgtgggc gatctgggtg ccgatgtcca tcgtccagcg ccgcggctcg agggcgtcga
     4621 ggtactcggg gaaggtctcc cagctccagt cgatgccctc ggcgagcgcc gtgccgggga
     4681 tgtcctcgac accctccatc aacccgatca gccagtcgcg gcgctccggt acgaccgggg
     4741 cgaacccgac gccgcagttg cccatgacca gggtcgtgac cccgtgccat gcgctggggg
     4801 cgagcatcga gtcccaggtg gcctggccgt cgtagtgcgt gtggatgtcg acccaccccg
     4861 gcgtgaccaa cagcccggtc gcgtcgatct
//
LOCUS       DAOSOA010000258         4870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17491_length_4870_cov_5.590239, whole genome shotgun sequence.
ACCESSION   DAOSOA010000258 DAOSOA010000000
VERSION     DAOSOA010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17491_length_4870_cov_5.590239"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKY13_13480"
     CDS             complement(<1..336)
                     /locus_tag="PKY13_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT80998.1"
                     /translation="MVASLGHSGAEADVARDAFAAGARMVTHLFNGMSGLDHRHPGVA
                     AAALLDDAVTAGLIADGVHVHPELVRLAFRTKGPGRIALVTDAVGWRAGTVGHLEIAH
                     DGTAPRLADG"
     gene            521..3043
                     /locus_tag="PKY13_13485"
     CDS             521..3043
                     /locus_tag="PKY13_13485"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT80999.1"
                     /translation="MQKFWSRLAVELGRRAGLVAIIGLLITLTMGYGITKLSFATGTE
                     SYLNQDDAAYAASVDYQKRFGGEANLTVITMDEGHTVAELITNPKNREAIEATSAELR
                     KVPGVRGVIDPIVALDLSANLISADPAAPTLQNLLGSVAARSLAKAIEQAPEADKAAR
                     EADNALTTQKVLAIPEEDRFLPKPAWTDFLLHDNQGEIRLSQRVVFPDDTHAMILTRF
                     DGNLKVDPATTATNTTYEIVENLELEHAEVRTTGSPKLLVEINDYLRGGMLQLGAIAL
                     GIMVLILLVLFNVRWRLLPLGVIVVGVIWAFGTAGYLGVPLTLVTVAGLPVMLGIGID
                     YAIQMHARVEEEAVIGRSAHPIQETARNLGPALLVVTFDAVFAFAALHWAAVPMLRDF
                     GLLLAVGVAVICIASIIVPLATLGIREYRKPTPKGEFNAGWLGTLVVKLGGLPAVAAI
                     PLAVASIAIFIGGISVEEKLHIQTDPVKWVNPESQVIKNLRYAEKEVGGSSELGVFIE
                     TKDPFSQEVVDHVDEFTNRYLGKYQPTGDIVVASSIVAIVGDLLVVPGTERVHPDAEL
                     VKEALAVAPADIKSTVFDDSGSTDQAYNIAFLTGPGSLEDSKTIVEEIRNEENPTSGA
                     DKPPAGTTVTPSGLVVVGVGLLDNLEKNRILLTYLSIAFVGIFLTIRMRSLIRSLLSL
                     VPVLIAVGVSSLVAYWANLELSPMTAVGGPLVVAACTEFTSLILLRFVEERGRGLDPK
                     EAVDVTAERTGRAFIVSACTAIAGVAVISFSSMPLLRDFGRVVGMNVAVALLSALVVL
                     PPMLVWADKWGWVSKGMIHDHHEPLIEFDGETDGSGGAPAPA"
     gene            3235..3801
                     /locus_tag="PKY13_13490"
     CDS             3235..3801
                     /locus_tag="PKY13_13490"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT81000.1"
                     /translation="MAVAALERGGEQAIKVREIANALDVSVGAVYHHFESRRDLIVAA
                     RIAQFEAVITGDVDAVRDLVERSETVDELWSGMQFLTRAAHSDARARSRRLRAEVAGV
                     ALHHPDLSAGLARAQDACTTAFTEIIVEGQRKGLVRRDLDARAVATLLQGVSLGLVLN
                     DINLVTPMDAESWFALTDQVYRSLLVTD"
     gene            complement(3832..>4870)
                     /locus_tag="PKY13_13495"
     CDS             complement(3832..>4870)
                     /locus_tag="PKY13_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT81001.1"
                     /translation="RYQRITTPLGGLLVPNDLWRVMYTSTDTNGSPILVTGLVRVPMD
                     RPAPPNGRPVVTWAHGTTGTADTCSPSRRLDAPPVPDPFVNDGFVVAATDYAGLGTPG
                     PHPYFDGASAGRAVLDIVTAAGHLPGVTIAPQFAIWGHSQGGHAALFARELAAQRLPA
                     HQLIGTVALAPPSLVKDTMTAILVALQPKGFAAMTVAGIAATHPDAELTDILIPAAAE
                     AIGTLVETECAAGIDRALGLFGNTDIVVANPNQIEPWATYLQAAEPAMAAGAGPLLLI
                     HSIDDRTVPAFMSAVVEMRTCSRGEPTVRWVLGSGGHNGIIGLTFDLDRQWTLDRFAG
                     VPAPSNCASGG"
BASE COUNT          718 a         1652 c         1740 g          760 t
ORIGIN      
        1 gccgtcggcg aggcggggcg cggtgccgtc gtgggcgatc tccaggtggc ccacggtgcc
       61 ggcccgccag cccaccgcgt cggtgaccag ggcgatgcgc ccggggccct tggtgcggaa
      121 cgcgaggcgc accagctcgg ggtgcacgtg cacgccgtcg gcgatgagtc cggcggtgac
      181 ggcgtcgtcg agcagggcgg cggcggccac gcccgggtgg cggtggtcca ggccggacat
      241 gccgttgaac aggtgcgtga ccatccgggc gccggcggcg aacgcgtctc gggccacgtc
      301 ggcctcggcc cccgagtggc ccagcgacgc caccacgccg cgctcggcgg cccacgccca
      361 ggcctcctcg gcgccgtcgc actcgggtgg cccgacggcg acgaccggcc ctcggcgtcg
      421 gccggcagac gccgcccgac accacgggag ttcatccgtc gcgggggctg gtgaacgact
      481 acagttcaac cgccgttcac ccgagttgga gattgatgtg atgcagaagt tctggtcgcg
      541 actggccgtc gagctcggga ggcgggcggg tctcgtggcc atcatcggcc tcctgatcac
      601 cctcaccatg gggtacggga tcacgaagct gagcttcgcg acgggcaccg agagctacct
      661 gaaccaggac gacgcggcct acgccgcgag cgtcgactac cagaagcgct tcggcggcga
      721 agcgaacctg acggtcatca cgatggacga gggccacacg gtcgccgagc tcatcaccaa
      781 ccccaagaac cgcgaggcga tcgaggcgac gagcgccgag cttcgcaagg tgcccggcgt
      841 gcgcggcgtc atcgatccga tcgtggcgct cgatctctcc gccaacctca tctcggccga
      901 cccggcggcc ccgacgctgc agaacctgct cggttcggtc gcggcgcgat cgctggccaa
      961 ggcgatcgag caggctcccg aggccgacaa ggccgcgcgc gaggccgaca acgcgctgac
     1021 cacgcagaag gtgctcgcga tccccgaaga ggaccggttc ctcccgaaac cggcctggac
     1081 cgatttcctg ctccatgaca accagggcga gattcgcctg tcccaacgtg tggtgttccc
     1141 cgacgacacc cacgccatga tcctcacccg gttcgacggc aacctgaagg tcgacccggc
     1201 cacaacggcc accaacacga cctacgagat cgtcgagaac ctcgagctgg agcacgccga
     1261 ggtgcggacg accgggtccc ccaagctcct cgtcgagatc aacgactacc tccgcggcgg
     1321 catgttgcag ctcggcgcga tcgcgctggg catcatggtg ctgatcctgc tcgtgctgtt
     1381 caacgtgcgt tggcgcctgc tgccgctcgg cgtgatcgtc gtcggtgtca tctgggcgtt
     1441 cggcaccgcc ggctacctcg gggtgcctct cacgctcgtc accgtcgccg gcctgcccgt
     1501 catgttgggc atcggtatcg actacgcgat ccagatgcac gcccgcgtcg aggaggaagc
     1561 ggtgatcggg cgctcggcgc accccattca ggagacggcc cggaacctcg gtccggccct
     1621 cctcgtcgtc acgttcgacg ccgtcttcgc cttcgccgcc ctgcactggg cggcggtccc
     1681 gatgctccgc gacttcggcc tgctcctggc cgtgggcgtc gcggtgatct gcatcgcctc
     1741 gatcatcgtt ccgctcgcca cgctcggcat ccgcgagtac cgcaagccga cccccaaggg
     1801 tgagttcaac gccgggtggc tgggcaccct ggtcgtgaag ctgggcgggc tcccggcagt
     1861 ggcggcgatc ccgctcgccg tcgcctcgat cgccatcttc atcgggggca tcagcgtcga
     1921 ggagaagctg cacatccaga ccgatccggt gaagtgggtc aaccccgaga gccaggtcat
     1981 caagaacctg cgctacgccg agaaggaggt cggaggttcg agcgagctcg gtgtgttcat
     2041 cgagacgaag gacccgttct cccaggaggt cgtcgatcac gtcgacgagt tcaccaaccg
     2101 gtatctcggc aagtaccagc cgacgggcga catcgtcgtg gcgtcgagca tcgtggcgat
     2161 cgtcggcgac ctgctcgtgg tgccgggcac cgagcgggtc catcccgacg ccgaactcgt
     2221 gaaggaggcc ctcgcggtcg cacccgctga catcaagtcg acggtgttcg atgacagcgg
     2281 ttcgaccgac caggcgtaca acatcgcgtt cctgaccggc cccggctcgc tcgaggacag
     2341 caagacgatc gtcgaggaga tccgcaacga ggagaacccg acctcgggcg ccgacaagcc
     2401 gccggccggc acgacggtga cgccgtcggg cctcgtcgtc gtgggggtgg ggctcctcga
     2461 caatctcgag aagaaccgca tcctgctcac ctacctgtcg atcgcgttcg tgggcatctt
     2521 cctcacgatc cggatgcgga gcctgatccg ctcgctgctg tcgctcgtgc ccgtgctgat
     2581 cgccgttggc gtgtcgtcgc tcgtcgccta ctgggccaac ctcgaactgt cgccgatgac
     2641 cgcggtcggt ggtccgctcg tggtcgcggc ctgcaccgag ttcacgtcgc tcatcctcct
     2701 gcggttcgtc gaggaacggg gccggggcct cgacccgaag gaagcggtcg acgtgacggc
     2761 ggagcggacg gggcgggcgt tcatcgtgtc ggcgtgtacc gccatcgccg gtgtcgccgt
     2821 catctcgttc tcgtcgatgc cgctcctgcg tgacttcggt cgggtggtcg gtatgaacgt
     2881 cgcggtcgcc ctcctgagcg ccctcgtggt gctcccgccg atgctggtgt gggcggacaa
     2941 gtgggggtgg gtgtcgaagg gcatgatcca cgaccaccac gaaccgttga tcgagttcga
     3001 cggcgagacc gacggttccg gcggggcacc ggctcccgcc tgatcgtcga accgctggcc
     3061 acccgtttcg ggtgggcatc gcggcgcgtc gggatcgcgg gcgttaccct gacccggcgg
     3121 tcgttccctt gcggccagcg gtcacgattc gaggtcgata tgccgcgtac ccaggtacgt
     3181 gctgctgcag cacgtgaaca gcagggcgag gcgactcgcg gtcgattggt cgaggtggcg
     3241 gtcgcagcgc tcgaacgcgg cggcgagcag gccatcaagg tgcgcgagat cgccaacgcg
     3301 ctcgacgtgt cggtcggcgc ggtctaccac cacttcgaga gccgccgcga cctcatcgtc
     3361 gccgcccgca tcgcccagtt cgaggcggtg atcaccggcg acgtcgacgc ggtgcgtgac
     3421 ctggtcgagc gctcggagac ggtcgacgaa ctctggagcg gcatgcagtt cctgacgcgg
     3481 gctgcccaca gcgacgcgcg ggcgcgatcg cgccggctcc gcgccgaggt cgcgggcgtg
     3541 gccctccatc acccggatct gagcgccggc ctggcccggg cccaggacgc ctgcaccacg
     3601 gcgttcaccg agatcatcgt cgagggccaa cgcaaggggc tggtgcgtcg cgacctcgat
     3661 gctcgagcgg tggcgaccct cctgcagggc gtctcgctgg gtctggtcct gaacgacatc
     3721 aacctcgtga ctccgatgga tgccgagagt tggttcgccc tcaccgacca ggtgtaccga
     3781 tcgctgctcg tcaccgactg acgtcgacac gggcgcctcg tcggcggacc gtcagcctcc
     3841 ggacgcgcag ttggacggtg cggggacgcc ggcgaatcgg tcgagcgtcc actggcggtc
     3901 gaggtcgaat gtgaggccga tgatgccgtt gtgcccgccg ctcccgagca cccaccggac
     3961 ggtcggctcg ccgcgcgagc acgtgcgcat ctcgacgacg gcagacatga aggccggcac
     4021 cgtccggtcg tcgatggagt ggatcaggag gagcgggccg gcgcccgccg ccatcgccgg
     4081 ctcagcggcc tgcaggtagg tcgcccacgg ttcgatctgg ttcgggttgg cgacgacgat
     4141 gtcggtgttg ccgaagaggc cgagcgcccg gtcgatcccg gcggcgcact ccgtctcgac
     4201 gagcgtcccg atcgcttcgg cagcggccgg aatgaggatg tcggtgagct cggcgtcggg
     4261 gtgggttgct gcgatgccgg cgacggtcat cgccgcgaac cccttcggtt ggagggcgac
     4321 gaggatcgcc gtcatggtgt ccttgacgag gctcggcggt gcgagcgcca cggtcccgat
     4381 gagctggtgt gccggcaggc gctgggcggc gagctcgcga gcgaacagtg ccgcgtgtcc
     4441 gccctgggag tggccccaga tggcgaactg cggggcgatc gtgacgcccg ggaggtggcc
     4501 ggcagccgtc acgatgtcga gcaccgcccg accggcgctg gcgccgtcga agtacgggtg
     4561 gggcccggga gtgccgaggc ccgcgtagtc ggtcgcggcg acgacgaagc cgtcgttgac
     4621 gaagggatcg ggcaccggag gagcgtcgag ccgtctcgac gggctgcacg tgtcggcagt
     4681 gccggtggtg ccgtgggccc aggtcacgac ggggcggccg ttcggcggtg caggtcggtc
     4741 catcgggacg cggacgagcc cggtgacgag gatcggtgag ccgttggtgt cggtcgacgt
     4801 gtacatgacc cgccacaggt cgttggggac gagcagcccc ccgagtgggg tcgtgatccg
     4861 ttggtagcgg
//
LOCUS       DAOSOA010000259         4860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17526_length_4860_cov_7.613736, whole genome shotgun sequence.
ACCESSION   DAOSOA010000259 DAOSOA010000000
VERSION     DAOSOA010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17526_length_4860_cov_7.613736"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1251
                     /locus_tag="PKY13_13500"
     CDS             142..1251
                     /locus_tag="PKY13_13500"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT81002.1"
                     /translation="MTGVTDSLVATVAARADEIETAGRLPADLARAFAEAGLFRQLVP
                     ASLGGGESHPRALVESIERVSAADGSAGWCVMIASTTAAMSGWLPAAEAAEVFGDPLG
                     VWGGAYAPLGRATREGDQWRLSGTWPWGSGSQNCTWMAGGAVCDDGVFRLLYVPIADV
                     TIIENWDVVGLRGTGSHDWSVTDALVPVGRSVDLLGGSPVEPGPLYQFPLFGLLALGI
                     ASVSLGLGAAALDALAELAGAKTPSLQRRKLAERGTVQAEVARSTAQLRSARAYVLEA
                     IDEAWTSAVDGHPPTIDQRANLRLAATHAAHTAVEVVRSAFTLAGGTAVRNDSPLARI
                     LRDANVVTQHLMVSPVTYEPVGRVLLGLPVELLDL"
     gene            complement(1302..2000)
                     /locus_tag="PKY13_13505"
     CDS             complement(1302..2000)
                     /locus_tag="PKY13_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81003.1"
                     /translation="MTNPRRLDPEAVSATAERLYLRVQERFPDRGISRLANELVTIAE
                     ETRARVGDLRRPRRALRAGIAFAAALVLTAIVVAATQVDSGARVGGVNDFVGFVESLI
                     QDVVFLGLGAAFLFGIEGRLKRRTALAGLHDLRSFAHVIDMHQLTKDPEFVISDLQPT
                     SHSPDRIDDRVLLGRYLEYCSEMLALTSKLAASYAFDNSDAVVLGAIREIQELVGLLS
                     GKIWQKRVILDTAS"
     gene            complement(1997..2680)
                     /locus_tag="PKY13_13510"
     CDS             complement(1997..2680)
                     /locus_tag="PKY13_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT81004.1"
                     /translation="MTDLDPPPIDLLDGIMTTRALRRYTDEPVSDDDLWTILRAAQQG
                     PSGGNIQPWQFVVVTDDEPKAKLGELYRSSYYRYEAAMLAMSPPRRPEDEPSWQRTIA
                     ASRHLADHFGEAPAIIGAAMADISMDVVDEDGVMDVGTPYASVYPALQNLMLAARALG
                     LGTALTTVFRIRHDEVRAVLGVPDRFQVVALIPVGHPVGSFGRARRRPVEKVTHWNTW
                     GERREPPTP"
     gene            complement(2693..3496)
                     /locus_tag="PKY13_13515"
     CDS             complement(2693..3496)
                     /locus_tag="PKY13_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT81005.1"
                     /translation="MIDTAGYSTLHFELLDDDTILRITIDRPDSAVNAVDEALHHDLT
                     RVFGALRSERELRAIVLTGSGGAFSAGGDFDWFPTLRTPERLAALRVDAKTMIWDLLD
                     IEVPIIAAIGGPAIGLGASIALLCDVIVAAEGVRIADPHVKVGLVAGDGGAVIWPLVL
                     GPALAKEYLLTGKPLLAEDAARLGLVNHVVAAGELANTALDLARQIAANPPLAVRYTK
                     AAVNKGIRERMETLFDYASALELSTFLSDDHAEAVAAAVERRPPHFTGH"
     gene            complement(3513..4646)
                     /locus_tag="PKY13_13520"
     CDS             complement(3513..4646)
                     /locus_tag="PKY13_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT81006.1"
                     /translation="MDFELPVDDDPRRLAVRAWVSAHPDPTPVELADAGYSAPHWPRP
                     WGLDADAEHQLIISQELDRAGIGPVDNQIGIGWAGPTLLFAGTEEQKQRYLRPMLTCD
                     EFWCQLFSEPEAGSDLSSLTTRAVLDGDEWIVNGQKVWSTWAEKSEFGILLARTDPTA
                     PKHKGISYFICPMDQPGIDIRPIREMSGGHHFNEVFFDGARIPAANLVGAPGDGWRLA
                     RVTLGNERVSLSHGGVCWGMGPTSDDFFDAVASTGGIDDPHLRQRAAAMYTEAFLLQL
                     HDRKILSQTLAGTEPGPEASLKKLMADEHGQKLTDLAADLCGAQGMLAGEGRSGPFGG
                     GEHEQWPWAQLFARALTVGGGTTQVQRNILAERVLGLPRDEAR"
BASE COUNT          676 a         1616 c         1796 g          772 t
ORIGIN      
        1 gccgatctcg gtgatgcccg cgtgcatcgt gccgctcggc tggccgcaag gccgttacgg
       61 ccccaccacc cgtcgcccgg tcggcgacgt cacccacctc gaccggttcg gcaaccagcc
      121 gtggaagggc tcggcgtcga catgacggga gtgaccgact cgctcgtggc caccgtcgcg
      181 gcacgcgccg acgagatcga gaccgccggt cgtctgcctg ccgacctggc ccgggcgttc
      241 gccgaggcgg gcctgttccg acagttggta ccggcttccc tcggtggcgg cgagtcgcac
      301 ccccgggccc tcgtcgagtc gatcgagcga gtgtcggcgg ccgatgggtc ggccggttgg
      361 tgcgtgatga tcgcgtccac gaccgcagcg atgtcggggt ggctcccggc ggccgaggcc
      421 gccgaggtgt tcggcgaccc gctcggagtg tggggcgggg cgtacgcgcc gctcggccgg
      481 gcgacccgcg agggcgacca gtggcgactg agcggcacct ggccgtgggg gagcggctcg
      541 cagaactgca cgtggatggc gggcggcgcc gtatgcgacg acggggtgtt ccgcttgttg
      601 tacgtgccga ttgccgacgt cacgatcatc gagaactggg atgtcgtcgg cctgcgcggc
      661 acgggcagtc acgactggtc ggtcaccgac gcgctcgttc cggtcgggcg gtcggtcgat
      721 ctcctgggcg ggtcgcccgt ggaacccggc ccgctgtacc agttcccgct gttcggtctg
      781 ttggcgctcg gcatcgcctc ggtgtcgctc ggcctggggg ccgcagcgct cgatgccctc
      841 gccgagctgg ccggggcgaa gaccccgtcc ctgcaacgac gcaagctcgc cgagcgcggc
      901 accgttcagg ccgaggtggc ccgctcgacg gcccagctgc ggtcggcgcg ggcgtatgtg
      961 ctcgaggcga tcgacgaggc gtggacctcc gcggtcgacg gccacccgcc gaccatcgat
     1021 cagcgggcca acctgcggct ggcggcgacc cacgcggcgc acaccgctgt cgaggtggtg
     1081 cgatcggcct tcacgctcgc gggtggcacg gcggttcgaa acgactcgcc gctcgctcgc
     1141 atcctccgcg acgccaacgt cgtcacgcag cacctgatgg tgtcgccggt gacctacgaa
     1201 ccggtcggac gcgtgctgct cgggctgccg gtggagctgc tcgacctctg atccccgttg
     1261 agcttggagg gtggaccact ccgccgttcc gccgggcggg ttcagctcgc ggtgtcgagg
     1321 atgacgcgct tctgccagat ctttcctgac aggagaccga cgagctcttg gatctcgcga
     1381 attgcgccca gcaccacggc gtcgctgttg tcgaacgcgt aggacgcggc gagcttgctc
     1441 gtgagcgcca gcatctcgct gcagtactcg agataccggc cgagcagcac ccggtcgtcg
     1501 atccggtccg gggaatggct cgtcggctgg aggtcgctga tgacgaactc cgggtccttc
     1561 gtgagctggt gcatgtcgat gacgtgggcg aagctccgca gatcgtgcaa cccggccagc
     1621 gccgtccttc gtttcaggcg gccctcgata ccgaagagga acgctgcgcc gaggccgagg
     1681 aacaccacgt cctggatcaa gctctcgacg aaaccgacga agtcgttgac cccgccgacg
     1741 cgggcacccg agtccacctg cgtcgccgcc acgacgatcg cggtgagcac caacgccgcg
     1801 gcgaacgcga tgccggcgcg aagggctcgg cgggggcgcc ggaggtcgcc gacacgggcc
     1861 cgtgtttcct ccgcgatcgt taccagttca ttcgcgagac ggctgatgcc acggtcgggg
     1921 aaccgttcct gcactcgcag gtacaggcgc tcggcggtgg cggacacggc ttcggggtcg
     1981 agccgtctcg ggttggtcat ggggtcgggg gctcccgccg ctcgccccac gtgttccagt
     2041 gggtgacctt ctcgacgggg cgccgccggg cacgtccgaa cgatccgacc gggtggccga
     2101 ccgggatcag ggcgacgacc tggaaacgat ccggcacgcc cagcacggcg cgcacctcgt
     2161 cgtggcgaat gcggaagacc gtcgtgagcg ccgtgccgag accgagggcg cgggccgcga
     2221 gcatcaggtt ctgcagcgcc ggatacaccg acgcgtacgg ggtgcccacg tccatcaccc
     2281 cgtcctcgtc gacgacgtcc atcgagatgt cggccatcgc cgcgccgatg atcgccggcg
     2341 cctcgccgaa gtggtccgcc agatgtcggg acgccgcgat ggtccgctgc cacgacggtt
     2401 cgtcctcggg acgccgagga gggctcatcg cgagcatcgc cgcttcgtac cggtagtagg
     2461 agctgcgata gagctcgccg agcttggcct tcggctcgtc gtcggtcacg accacgaact
     2521 gccacggctg gatgttgccg cccgacgggc cctgctgggc ggcgcgcagg atcgtccaga
     2581 ggtcatcgtc ggagacgggt tcgtcggtgt atcgacggag ggcccgggtg gtcatgatcc
     2641 cgtcgagcag gtcgatcggg ggtgggtcga ggtcggtcat cgcgctcctt cgtcagtggc
     2701 ccgtgaagtg gggcggccgg cgttcgaccg ctgcggcgac cgcctcggcg tggtcgtcgc
     2761 tcaggaacgt ggacagctcg agcgccgacg cgtagtcgaa cagcgtttcc atacgctcac
     2821 ggatgccctt gttgacggca gccttcgtgt agcggacggc caacggcggg ttcgccgcga
     2881 tctgccgggc gaggtcgagc gctgtgttcg cgagctcgcc tgcggccacg acgtggttca
     2941 cgagaccgag ccgggccgca tcctcggcga ggagcggctt gcccgtcagg aggtactcct
     3001 tcgcgagcgc cggaccgagg acgaggggcc agatgaccgc cccaccgtcg ccggcgacca
     3061 ggccgacctt cacgtggggg tcggcgatgc ggaccccttc cgcggcgacg atgacgtcgc
     3121 agagcagcgc gatcgacgcg ccgagaccga tcgccggtcc gccgatcgcc gcgatgatcg
     3181 gcacctcgat atcgaggagg tcccagatca tcgtcttggc gtcgacgcgg agggcggcga
     3241 gtcgctccgg cgtgcggagt gtgggaaacc agtcgaagtc gccgccggcg ctgaacgcgc
     3301 cgccggatcc ggtcagcacg atggcgcgca gttcgcgttc gctgcgcagc gccccgaaca
     3361 cgcgggtgag atcgtggtgg agcgcttcgt cgaccgcgtt cacggcggaa tcgggtcggt
     3421 cgatcgtgat gcggaggatc gtgtcgtcgt ccaacagctc gaagtgcagg gtcgagtagc
     3481 cggcggtgtc gatcatgtgg gggagccgat catcagcgtg cctcgtcgcg gggaaggccg
     3541 agcacacgct cggcgaggat gttgcgctgc acctgcgtgg tgccgcctcc gacggtcagc
     3601 gcccgggcga acagctgcgc ccagggccac tgctcgtgct cgccgccgcc gaacggtccg
     3661 gagcggccct cgccggcgag catgccctgc gcgccgcaca ggtcggcggc gagatcggtg
     3721 agcttctgcc cgtgctcgtc ggccatgagc ttcttgagag acgcctccgg acccggctcg
     3781 gtgccggcca gggtttggct gaggatcttc cggtcgtgca gttggaggag gaacgcctcg
     3841 gtgtacatcg cggcggcgcg ctggcggagg tgcgggtcgt cgatcccgcc ggtcgacgcg
     3901 acggcgtcga agaagtcgtc gctcgtcggc cccatgcccc agcagacgcc gccgtgggac
     3961 agcgagacgc gctcgttgcc gagcgtgacc cgggcgagcc gccatccgtc gcccggagcg
     4021 ccgacgaggt tggcggcggg gatgcgggcg ccgtcgaaga acacctcgtt gaagtggtgg
     4081 ccccccgaca tctcccggat cgggcggatg tcgataccag gctggtccat cggacagatg
     4141 aaataggaga tgcccttgtg cttcggcgcg gtggggtcgg tgcgggcgag caggatgccg
     4201 aactccgatt tctcggccca ggtcgaccag accttctggc cgttgacgat ccactcgtcg
     4261 ccgtcgagca cggcgcgggt cgtgagcgag gagaggtcgc tcccggcctc gggttcgctg
     4321 aacagctggc accagaactc gtcgcacgtg agcatcgggc ggaggtaccg ctgcttctgc
     4381 tcctcggtgc cggcgaacaa caaggtggga ccggcccagc cgatgccgat ctggttgtcg
     4441 acgggaccga tgccggcgcg gtcgagttcc tgactgatga tcagctggtg ttcggcgtcc
     4501 gcatcgaggc cccagggccg gggccagtgc ggtgcggagt agccggcgtc ggcgagttcg
     4561 acgggcgtgg gatcgggatg ggcgctcacc cacgctcgca cggccaggcg gcgcgggtcg
     4621 tcgtcgacgg gcagctcgaa gtccacggcc gaaccctacg gcaccccgtt gggagggagt
     4681 cggccgaact gctgccacac tgtcaggcgc atggagacga cggtggtgga ccgactggga
     4741 gcgcacgcgt cggcgtggga cgcccttgtc gactcgatgc cgttgccgtc gccgttcctc
     4801 cgctcgtggt ggttggctga ggtcgcgatc ggtgaccctc ggatcgttct ggtgttcgac
//
LOCUS       DAOSOA010000260         4859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17534_length_4859_cov_4.361157, whole genome shotgun sequence.
ACCESSION   DAOSOA010000260 DAOSOA010000000
VERSION     DAOSOA010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17534_length_4859_cov_4.361157"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKY13_13525"
     CDS             complement(<1..327)
                     /locus_tag="PKY13_13525"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT81007.1"
                     /translation="MTVPVRLLVATLAVLTTALGVTAVRAAAAGNAPVAGRDVARAFR
                     GTSISINVTSNDYDPDGESFSLVSVATPAHGSVSSIGSSSFTYTPVSTFSGTETITYT
                     IRDSANT"
     gene            complement(480..1262)
                     /locus_tag="PKY13_13530"
     CDS             complement(480..1262)
                     /locus_tag="PKY13_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOT81008.1"
                     /translation="MLISTIAVACVPAASSPRPSISGDPVVGATLSVSSGAWTNDPTT
                     FHYSWESCTTVGVGCVRVGGDRNTYLITAGDLGRHIRATVTATNAAGSGWSGSDSVGP
                     VTSGEVPSVDCDVRGPGADLHGCDLSEEDLSETDLTGANLDGADLTGANLSGALLGAV
                     SAQAANFYQADLSGADLSGAGLNGANLEYTDLSTADLSGASAELASFFQANAVGAVMV
                     DIDLTGADLTGADLTDADLTGAVFSNTTCPTGAVVSAPGVCS"
     gene            1463..2014
                     /locus_tag="PKY13_13535"
     CDS             1463..2014
                     /locus_tag="PKY13_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81009.1"
                     /translation="MAQSAGAHVAVCTSSDLLESLLCELTAAGRTVIDHPPAAACWPA
                     AAADSTVYVATITADDASTEPWALVTSAARGVSLAVCITPRRLAVDLADQIRRVAPTA
                     WYDSSHRPIVHGLDADQIRLLGALHRGDDVRTAAREAGLSQRTAARRVAAARVTLGED
                     STVSAALRVGARLDELRRSPRSV"
     gene            complement(2011..4509)
                     /locus_tag="PKY13_13540"
     CDS             complement(2011..4509)
                     /locus_tag="PKY13_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT81010.1"
                     /translation="MGEYRTVVLTGAPGTGKTSLLDGLAFELRADGTTVHQVRCLPAL
                     QDRRLGALDALVDSGGDAERLVRAVDRDDVVVVDDLQWMDPDSLAALATLHGYCAVVA
                     GSLPIEARSTPQHGLSAVAHPVRIGALDPLATARLVEAYGPDLLPEERNRIVSASAGN
                     PFHAGLLVAFADTERSAPPASMSVHVDRAAALLARLGREERITLGALGRAGTALPAAC
                     LPGRIGLLAGLDLVVVDDDRVSLACEPLGRPEVQLLDDDEVAAVTALVASADALDDVV
                     RARALADAGDLVGASELARDVAGRPVEPGTLAEALRIAATVERRRRPSSTDHQLDGEV
                     QDLLIAAAAALNGDLRHREALEVLSGTAWSDDRQGRATIELMYAATNVGDRAVLADVI
                     GRVGSTLSQMDPPVAESMRSWVARMTLGDRAAAERRRGDIVGTRDFLVRAALDATARQ
                     ASDEDPGSGPRSTGAPVHDAFLDAVLRDELDAARNHAMILVNIGLHAEAQALARSSAA
                     RADAAGSANWRSEFTTIEMMSRFHAGAANDEALAWFSTVRTAPVRVNTRAVATVGLVT
                     ALADRGEVDRSGAILEPWLRVVESFDVITASAIGWAAVQRAWILGDLSSTIDLARSIT
                     ERSPDWVPGLAGLQVAWRWAEFESGVPITAPMPTGGLLDCATLEATAIDLAAAGRHDE
                     AATQFEVAASSWDRILVRCALRCRWARGVQLGRSGDRTTAEEVLTDLDGSLDGWGSPA
                     LRPRVRAALAEVAGRPVAATRSRGDIVTGRERAVLLLVAEGLTSVEIGTRLGVSPTTV
                     DSHVRSAVRKLGARNRRHAAALIDRRPSAEMPSG"
     gene            complement(4670..>4859)
                     /locus_tag="PKY13_13545"
     CDS             complement(4670..>4859)
                     /locus_tag="PKY13_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81011.1"
                     /translation="CDEMVVDGPPPDDLAERFLRARAIAASWNIHLLDWVACDDQLFR
                     SSRLAICPEETWWDLPSR"
BASE COUNT          765 a         1800 c         1614 g          680 t
ORIGIN      
        1 cgtgttggcg gaatcccgga tggtgtaggt gatggtctcg gtgcccgaga acgtcgacac
       61 cggcgtgtag gtgaagctgc tgctcccgat ggaggacacg gacccgtgcg ccggggtggc
      121 gaccgagacc aacgagaacg actcgccgtc ggggtcgtag tcgttggagg tcacgttgat
      181 ggagatcgac gttccccgga atgcccgagc gacgtcgcgc ccggccacgg gcgcattgcc
      241 ggcggcagcg gcgcggactg ccgtgacccc gagcgcggtg gtcagcacgg cgagcgtcgc
      301 gacgaggagg cgcaccggaa ccgtcaaccg atgccatcga ccgccccggt gatcccgctg
      361 ccgccgcagc gggatcaccg ttgcccgggg ttccgccacc cgatctccgc ccatcgttcc
      421 gcctctcgtg ttctcgccgc cgttgaatcc gccaaccggt ccgttcgttc gcaccggcat
      481 cagctgcaca cccccggtgc gctcacgacc gcgccggtcg gacaggtcgt gttcgagaac
      541 accgcccccg tcagatcggc atcggtgagg tccgcgccgg tgaggtcggc cccggtgagg
      601 tcgatgtcga ccatcaccgc accgacggcg ttcgcctgga agaagctcgc cagttccgcc
      661 gacgcccccg agaggtcggc cgtgctcagg tcggtgtact cgaggttcgc tccgttgagc
      721 cctgcacccg acaggtcggc tccgctcaga tcggcctggt agaagttcgc agcttgggcc
      781 gagaccgcgc cgaggagtgc tccgctcagg ttggcgccgg tcaggtcggc accatcgagg
      841 ttcgccccgg tcagatcggt ctccgacaag tcctcctccg acaggtcaca cccgtggagg
      901 tcggccccgg gaccccggac gtcgcagtcg accgacggga cctctccact ggtgacgggc
      961 ccgaccgaat ccgacccgga ccatcccgag ccggcagcgt tggtggccgt cacggtcgcc
     1021 cggatgtgtc ggcccagatc gccggccgtg atgaggtagg tgttccggtc tccgccgacg
     1081 cggacgcatc cgacgccgac cgtggtgcac gactcccacg agtagtggaa cgtcgtcggg
     1141 tcgttggtcc acgctcccga gctcacgctg agggtggcac cgacgaccgg gtcaccgctg
     1201 atggacgggc gaggcgacga tgccgctggg acacacgcca ccgcgatggt ggagatcagc
     1261 atcgcggccg ctcgcatcgg ccgcattcgt cgccgccgac ctccggctcg accgtgctcg
     1321 ctcatatcgc cccctgctcg cattgaagga ttccgccacc tcatggagcg catccttgtg
     1381 acgtcagccc ttgccgacct cctcaattgc tgccaacttt ggggcccgaa taccacgaat
     1441 cgggagaggt cgcaggttga acgtggcgca atccgccggc gcgcacgtcg ctgtgtgcac
     1501 cagctccgac ctcctcgaat cgttgctctg cgaactcacg gccgcgggcc gaaccgtcat
     1561 cgaccaccca cccgccgccg cctgttggcc tgccgccgcg gccgactcga cggtgtacgt
     1621 cgcgacgatc acggccgacg acgcgtccac cgaaccgtgg gccctcgtca cgtcagcggc
     1681 tcgcggcgtc tcgctggcgg tctgcatcac accacgccgc ttggcggtgg acctcgccga
     1741 ccagatccga cgagtagcgc cgacggcgtg gtacgactcg agccaccgcc cgatcgttca
     1801 cggtctcgac gccgaccaga tccggcttct gggtgccctg caccggggcg atgacgtgcg
     1861 cacggcagca cgcgaggccg gactcagcca acgcaccgcc gctcgacggg tcgccgccgc
     1921 tcgggtgacg ctcggggagg actcgaccgt gtcggccgca ctccgcgtcg gcgcccgact
     1981 cgacgagctc cgccgttcac cccggagcgt ctagcccgag ggcatctcgg ccgagggccg
     2041 acgatcgatc agggccgcgg catggcgacg attccgcgct cccaactttc gcacggccga
     2101 acgcacgtgg ctgtcgaccg tggtgggcga gacaccgagt cgggtaccga tctcgacgct
     2161 cgtcagccct tcggccacga ggagtaacac ggcccgctcg cgaccggtca cgatgtcacc
     2221 tcgggaccgg gtcgccgcga ccggccggcc tgcgacctcg gccagcgcag cgcgaaccct
     2281 cggtcgcaac gcagggctcc cccagccgtc gagagagccg tcgagatccg tgaggacctc
     2341 ctccgccgtg gtgcgatctc cgctcctccc gagctgcacc ccgcgcgccc accggcaccg
     2401 cagcgcacaa cgaacgagga tgcggtccca cgaggacgcc gccacctcga actgcgtcgc
     2461 cgcctcgtcg tggcgacccg ccgccgcgag gtcgatcgcg gtcgcctcga gcgtcgcaca
     2521 gtcgagcagg ccgccggtcg gcatcggcgc cgtgatcggt acgcccgatt cgaactccgc
     2581 ccatcgccac gccacctgga gaccggcgag tccggggacc cagtcgggag accgctcggt
     2641 gatcgagcgg gcaagatcga tggtggaact cagatcgccg aggatccaag cccgttgcac
     2701 cgccgcccaa ccaatcgcgg acgccgtgat gacgtcgaag gactcgacga cgcgcagcca
     2761 cggttcgagg atggcgccgc tgcggtccac ctcaccacga tcggcgagcg ctgtgacgag
     2821 ccccacggtc gcgaccgcac gggtgttcac tcggaccggc gccgtgcgta ccgtcgagaa
     2881 ccaggccagc gcctcgtcgt tggcggcccc cgcgtggaac cggctcatca tctcgatcgt
     2941 ggtgaactcg gagcgccagt tggcgctccc cgccgcatcg gcacgggccg cggacgaacg
     3001 ggccaacgcc tgcgcctcgg cgtggagccc gatgttcacg aggatcatgg cgtgattgcg
     3061 tgcggcatcg agctcatcgc ggagcacggc gtcgaggaat gcgtcgtgaa ccggcgcgcc
     3121 cgtgctgcga ggaccggagc ccgggtcctc gtcggacgcc tgcctcgccg tggcgtcgag
     3181 ggcagctcgg acgaggaaat cgcgtgttcc gacgatgtcg cccctgcggc gctccgcagc
     3241 cgcacgatcg cccaacgtca tccgcgccac ccaggaccgc atgctctcgg ccaccggggg
     3301 atccatctgc gagagggtcg agcccacccg accgatcacg tcggcgagca ccgcccgatc
     3361 accgacattg gtcgccgcgt acatcagttc gatcgtcgcg cgaccctgcc ggtcgtcgga
     3421 ccacgcggtg cccgacagga cctcgagcgc ctcgcggtgg cggaggtcgc cgttgagcgc
     3481 cgcagcggcg gcgatcagga gatcctgcac ctccccgtcg agttggtggt cggtcgacga
     3541 cggcctccgc cgtcgctcga cagtcgccgc gatccggagc gcctctgcca acgtccccgg
     3601 ctccacgggt cggccggcca catcccgagc cagctcactt gcacccacga ggtccccagc
     3661 gtccgccagt gccctggcac gaaccacatc gtcgagagcg tcggccgacg ccacgagcgc
     3721 cgtcactgcc gccacctcat cgtcgtcgag cagctgaacc tcagggcggc cgagcggttc
     3781 acatgccagg gacacacgat cgtcgtcgac gacgaccagg tcgagaccgg caagcagacc
     3841 gatccggccg ggaaggcatg cagcgggaag agcggttccg gcacgcccga gggctcccag
     3901 cgtgatccgc tcctcgcgac ccaaccgggc gagcagcgct gcggcccggt ccacgtgcac
     3961 cgacatcgat gccggaggag ctgatcgctc ggtgtctgcg aacgcgacca gcaaccccgc
     4021 gtggaacgga tttccggccg aagccgagac gatccgattc cgctcctccg gcaggaggtc
     4081 aggaccgtac gcctccacca gacgtgcggt ggcgagcggg tcgagcgccc cgatccgaac
     4141 cgggtgggcc acggcggaaa ggccgtgctg aggagtcgac ctcgcttcga tgggcaggga
     4201 accggcaacg acggcgcagt agccatgcag cgtcgccagc gcggcgagcg agtccgggtc
     4261 catccactgg aggtcatcga ccaccaccac atcgtcacgg tcgacggcgc gtacgagtcg
     4321 ctcggcatcg ccgccggaat ccacgagtgc gtcgagcgcg cccagtcgac gatcctgcag
     4381 cgctggcaga caccggacct ggtggaccgt cgtcccgtcg gcacgcagct cgaacgcaag
     4441 gccgtcgagg agcgaggtct tgccggtacc cggcgccccg gtcagcacga ccgttcgata
     4501 ctcacccacc gcccggcgca actcgcgagc caggtcgtgc cgaccgtcct gagtggagat
     4561 ccacgcccca ggtgccccgt cgaccatggc acacgatggc aggcgcgccc gttcgggtcc
     4621 accgcctcga cccaccggct cggcccaccg ctggtctcca ccgtcgacct caccgcgacg
     4681 gcaggtccca ccacgtctcc tcgggacaga tcgccaggcg actgctgcgg aacagctggt
     4741 cgtcgcacgc gacccagtcg agcaggtgga tgttccagga cgccgcgatc gcccgggcac
     4801 gaaggaaccg ctcggcgaga tcgtcgggcg gcggtccgtc gaccaccatc tcgtcgcag
//
LOCUS       DAOSOA010000261         4841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17596_length_4841_cov_4.789804, whole genome shotgun sequence.
ACCESSION   DAOSOA010000261 DAOSOA010000000
VERSION     DAOSOA010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17596_length_4841_cov_4.789804"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..598)
                     /locus_tag="PKY13_13550"
     CDS             complement(227..598)
                     /locus_tag="PKY13_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT81012.1"
                     /translation="MTDDQGSSEVDEPPPVECVIELRDTDGGVVVDVAGEIDEATRDF
                     LSERLSSAIDSGPTVQVDLTHVSFMDSWGLSAVLAAHADAVERGVAFEVSGASPQVAH
                     LFVITRVAVDYGWPTTFTSDD"
     gene            complement(606..1997)
                     /locus_tag="PKY13_13555"
     CDS             complement(606..1997)
                     /locus_tag="PKY13_13555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT81013.1"
                     /translation="MTDPEVTDVIAELDRRVRTDDLTQLGSKAQMVEWLETAASGSKL
                     ALLDLDDFRLLNQTLGHDAGDVLLTLVARRLERACPEPDYRVGRISQDEFAVLQVGAD
                     ADAEQLASVVASTFRDPFRVGGRAVGVRATCGITECAPTKSPTTVIREADTAMYEAKA
                     RAKSGALVFDEVYHRRAVEAFELADGLGRAVAGSEFDIHYQPEVDLARGRVVGAEALL
                     RWTNPRLGAVPPDTFIPIAETCGMIGELGAFVLRGALAQRRRWIAEGVVDDDFTMAVN
                     VSARQIDSDGFGDLVAWQLEVTGCPSRAALPGAHRVGDHGRSGGGRHPARAPGETGRS
                     AGDRRLRHRLLVAGLPPAPADHASEDRPEFRGPVGRASRGGAHRGDHRHRPGTGPGDG
                     GRGDRDARAAPAGDRPRLRRRAGVPLRPADAAERLRRRPARCGGAVGAIARSVRWAAE
                     LANGAALVVGTVG"
     gene            2134..2301
                     /locus_tag="PKY13_13560"
     CDS             2134..2301
                     /locus_tag="PKY13_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81014.1"
                     /translation="MTATSDVSPATRARAELQHAVQANQEALAAGENPPFGQGALIVL
                     RRRWEAAEASD"
     gene            complement(2351..>4841)
                     /locus_tag="PKY13_13565"
     CDS             complement(2351..>4841)
                     /locus_tag="PKY13_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT81015.1"
                     /translation="EDDDVGSGVFSADPDVVQAAVVAQRDLAGGTWLVLGVVVMMVLA
                     EQFPIDLELRHESHSFSFSKIPIVIGVFLFAPFVAVAARLVSSLIVLCGFQRQPPMKL
                     LINLLGHMLEVMVAATVVIALRPPGPLGPAAWGVAVLAVTAADLVAALVISAAISAFQ
                     GRWEPGLMDGVVAGLIAGIPDVAFAIAMVTMLEAHHAVAWVVLGSGVAFVIVSRAYSA
                     VSARYRAMQLLDRFTRAISGAVIDGTATASLLQESAEILHADAAWLIVAEPERTVRVT
                     VVDGETVEESAHPIDLRIIGEPGEAARLETEDGPLEDLLRSDGRSEVIFCTVGHGSAK
                     TVILVVADRSGTVRPFDSEDVALFETLAAHAGLALLNVELVNQLRDEISTTEYQANHD
                     ALTGLPNRSLFQTRLRAAIDSGDRPAVLLIDLDRFKDVNDTLGHHNGDRLLEEIARRL
                     EVALGPDRCAARLGGDEFAALVTGSPARDEVEELALDLATQLERPVTVAGVAAEVGAS
                     IGIAFAVDGVTDPDTLLRQADVAMYTAKGDRSVVETYSADRDNYSPQRLSLVGRLRAA
                     IDGGELVLYYQPQIDLASGTVIGVEALVRWPQVGSTPIPPDEFIYIAEHTGLIRPLTR
                     LVLDLAVAQAKRWRDQGRDLRMSLNLSPHNLIEPGIVDTIAATLAAASVPPDRVMLEL
                     TESTVMANPKRTLGVLHELRELGVGIAIDDFGTGHSSLAYLTTLPATELKIDKSFVLA
                     IGADPTADSIVRSVIDLGRNLDLGVIAEGVETEHAATTLTRMGCRLAQGYYYSRPLEV
                     RAFEHWHATHDAALTAGRPAGRDAAIDTTAG"
BASE COUNT          815 a         1696 c         1638 g          692 t
ORIGIN      
        1 cgggagcata cgacaccacc acggagtcgc ccgccgacgg gcggccccga acccggccgg
       61 caaggaccca gaatgatgtg acaactgtca caggtttggg atccactcgc cggggtatcg
      121 gcgtcgtagc gacagtccga gacgatcccc tcagcgacgt cgtgagcctg cctcgagacc
      181 ctccggccct ccagcccgga gggtctcggc aagttcggcg tgaagctcag tcgtcggagg
      241 tgaaggtggt cggccacccg tagtcgacgg cgaccctggt gatcacgaac aggtgagcga
      301 cctgcggcga ggcacccgac acctcgaacg cgacaccccg ctcgaccgcg tcggcatggg
      361 cggcgagcac tgcgctgaga ccccacgagt ccatgaacga cacatgagtg aggtcgacct
      421 ggaccgttgg ccccgagtcg atcgcggacg acaggcgttc cgacaggaaa tcccgggtcg
      481 cctcgtcgat ctcgcccgcc acgtcgacaa cgaccccgcc gtcggtgtcg cgcaactcga
      541 tcacgcactc gacgggaggt ggctcgtcca cttcggaaga accctgatcg tcggtcacga
      601 ccccatcatc ccaccgttcc gacgacgagc gccgcaccgt tggccagttc ggcggcccag
      661 cggaccgacc gcgctatcgc gccgacggcg ccgccgcacc gagcaggcct gcggcgaagt
      721 cgctcggcgg cgtcggccgg gcgaagaggt acccctgccc gacgtcgcaa ccgaggtcgg
      781 tcaccagccg gcgctgctcg ggcgtctcga tcccctcggc caccgtcgcc aggcccagtg
      841 cccgggcgat gccgatggtc gcctcgatga gcgccgcccc gcgaggctcg gccgaccggg
      901 ccacgaaact ccggtcgatc ttcagatgcg tgatcggcag gtgctggagg taggccagcg
      961 acgagtagcc ggtgccgaag tcgtcgatcg ccagctgaac gcccagtctc gccaggcgcc
     1021 cgagccgggt gacggcctcc gccggatcgg ccatgatcgc cgactcggtg agctccaggc
     1081 agagccgctc gggagggaca tcccgtcacc tcgagctgcc acgccaccag atcaccgaag
     1141 ccgtcactgt cgatctgacg cgccgacacg ttgacagcca tcgtgaagtc gtcatccacg
     1201 accccctcgg cgatccaacg ccggcgctga gccaacgctc cccgcaggac gaacgcgccg
     1261 agctcgccga tcatcccgca cgtctcggcg atcgggatga acgtgtcggg cggcacggcc
     1321 ccgaggcgcg ggttcgtcca ccggagcagc gcctccgccc ccacaacccg tccccgagcc
     1381 aggtcgacct ccggctggta gtggatgtcg aactcgctcc cggcgacggc ccggccgagc
     1441 ccgtcggcga gctcgaacgc ttcgacggcg cgacggtggt acacctcgtc gaacaccagc
     1501 gcaccgctct tggcgcgtgc cttcgcctcg tacatggccg tgtccgcttc gcggatcacg
     1561 gtcgtgggcg acttcgtcgg agcgcattcc gtgatgccgc aggttgcgcg cacccccact
     1621 gctcggccgc cgacccggaa cggatcgcgg aacgtcgacg ccacgaccga cgccagctgc
     1681 tcggcgtcgg cgtcggcgcc cacctggagc accgcgaact cgtcctgact gatgcgtccg
     1741 acccggtagt ccggctcggg acaggctcgc tccaggcgcc gcgccaccag ggtcaagagg
     1801 acgtcgccgg cgtcgtggcc gagcgtctgg ttgaggagcc ggaagtcgtc gaggtcgagg
     1861 agggcgagct tcgaccccga ggcagcggtc tccagccact ccaccatctg cgccttgctg
     1921 ccgagctgcg tgaggtcatc ggtgcggacg cgccgatcga gctctgcgat cacgtcggtc
     1981 acctccgggt cggtcatgga aaggtggtcg ggttgttcat tcctgaacta aaggacgcgc
     2041 tcgctgttca cacagccgtc atcatcgcgg gtctgggccc gttccccccg atcagggggt
     2101 cgccgctgtc cccgattcgg gtaggaggcc tccatgaccg ccacctccga cgtctccccc
     2161 gccacccgag cccgagccga actccagcac gccgtgcaag ccaaccagga agcactcgcc
     2221 gccggggaga acccgccgtt cggccaaggt gcgctcattg tgctgcgtcg ccgatgggaa
     2281 gccgccgaag cctcggactg acggccaacg ggtcgaccgc gaccacgacc gccgacacgg
     2341 cggccggcgc tcaccccgca gtggtgtcga tcgccgcgtc tcgcccggca ggtcgacctg
     2401 cggtcagggc agcgtcgtgg gtggcatgcc agtgttcgaa ggcccggacc tcgagcggac
     2461 ggctgtagta gtagccctgg gccaagcgac aacccatgcg cgtcagcgtc gtcgctgcgt
     2521 gctcggtttc gacaccttcg gcgatgaccc cgaggtcgag gttccgaccc aggtcgatga
     2581 cggaacggac gatgctgtcc gccgtcgggt cggccccgat cgcgagcacg aacgacttgt
     2641 cgatcttgag ctcggtcgcc ggcagcgtcg tgagatacgc gagcgacgag tggcccgtcc
     2701 cgaaatcgtc gatcgcaatg ccgaccccca gttcacgaag ctcgtggagg acacccaggg
     2761 tccgcttggg attggccatc accgtcgatt ccgtcagctc cagcatgacc cggtcgggcg
     2821 gcaccgacgc tgcggcgagc gtcgctgcga tcgtgtcgac gatgccgggt tcgatgaggt
     2881 tgtggggcga caggttgagc gacatccgaa gatcccgacc ctggtcgcgc caacgcttgg
     2941 cctgggcaac ggcgaggtcc agcacgagcc gggtcagcgg acggatgagt ccggtgtgct
     3001 ctgcgatgta gatgaactcg tcgggcggaa tgggcgtgct gcccacctgc ggccaccgga
     3061 cgagcgcctc tacgccgatg acggtgcccg aggcgagatc gatctgcggt tggtagtaga
     3121 ggacgagttc tccgccgtcg atggcggctc gcaaccgacc gacgagggac aggcgctgcg
     3181 ggctgtagtt gtcacggtcg gcggagtagg tctccaccac cgaccgatca cccttcgcgg
     3241 tgtacatggc gacgtcggcc tgacgcagca aggtgtcggg atcggtgaca ccgtcgacgg
     3301 cgaacgcgat gccgatgctg gcgccgacct cggccgcgac cccggcaacg gtaaccggcc
     3361 gctcgagctg ggtggccaga tcgagagcga gttcctccac ctcgtcgcgt gcgggtgaac
     3421 cggtgacgag agcggcgaac tcgtcaccgc ccaggcgtgc ggcgcagcgg tccggaccga
     3481 gggccacctc gagacggcga gcgatctcct cgaggagccg gtcgccgttg tgatggccga
     3541 gcgtgtcgtt cacgtccttg aaccggtcca ggtcgatgag gaggacggcc ggccggtcac
     3601 cgctgtcgat cgctgcccgg aggcgcgtct ggaacaggct gcggttgggc aggccggtga
     3661 gcgcgtcgtg gttcgcctgg tactcggtcg tggagatctc gtcgcggagc tggttgacca
     3721 gttcgacgtt gagcagcgcg agcccggcgt gcgccgcgag cgtctcgaac agggcgacat
     3781 cctcggagtc gaacgggcgg acggtaccgc tgcggtcagc gacgacgagg atcaccgtct
     3841 tggcactgcc gtgaccgacc gtgcagaaga tgacctcgga ccggccatcg ctgcggagca
     3901 ggtcctcgag cggtccgtcc tcggtctcga gacgggcggc ctcaccgggt tcaccgatga
     3961 tccgcagatc gatcgggtgc gccgactcct cgaccgtctc accgtcgacg accgtgacgc
     4021 gcaccgtgcg ttcgggctcg gcgacgatga gccaggcggc atcggcgtgg aggatctcgg
     4081 cgctctcctg aaggagcgac gccgtcgcgg tgccgtcgat cacggcaccg ctgatcgccc
     4141 gggtgaaccg gtcgagcagc tgcatcgccc gataccgggc gctgaccgcg gagtaggcac
     4201 ggctcacgat cacgaatgcc acaccgctgc cgagcacgac ccacgcgacc gcgtggtgcg
     4261 cctcgagcat cgtgaccatg gcgatggcga acgccacgtc gggaatcccg gcgatcagac
     4321 cggcgaccac gccgtccatg agtccgggct cccaccgccc ctggaatgca ctgatggcgg
     4381 ccgagatcac gagggcggcg accagatccg ccgccgtcac ggcgagtacg gcgacgcccc
     4441 acgctgcggg accgagcggg cccggagggc gcaacgcgat caccaccgtg gcggcgacca
     4501 tcacctccag catgtggccc agcaggttga tgaggagctt catcggcggc tgccgctgga
     4561 acccgcacag cacgatcagc gacgacacga ggcgcgcggc cacggcaacg aacggcgcga
     4621 agaggaacac accgatcacg atcgggatct tcgagaacga gaacgaatgg ctctcgtggc
     4681 gcagttcgag gtcgatcggg aactgttcgg cgaggaccat catcaccacg acaccgagga
     4741 ccagccacgt accaccggca agatcacgct gcgccacaac agccgcctgc accacatcgg
     4801 gatcggccga gaacacgccc gaacccacgt catcgtcctc g
//
LOCUS       DAOSOA010000262         4834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17626_length_4834_cov_7.321197, whole genome shotgun sequence.
ACCESSION   DAOSOA010000262 DAOSOA010000000
VERSION     DAOSOA010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17626_length_4834_cov_7.321197"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..646
                     /locus_tag="PKY13_13570"
     CDS             197..646
                     /locus_tag="PKY13_13570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT81016.1"
                     /translation="MHVTAKADYAMRAIVEISVAPDGRAKAEQIASAQDIPLGFLRGI
                     LTDLRRSGLIVSQRSANGGFRLARPASEITIAEVVRSVEGPLALVRGLRPTELDYPGA
                     TAPLRDVWIALRANLRAVLDHTTIADVAEGRLPELVRSLAADPDSWL"
     gene            808..1263
                     /locus_tag="PKY13_13575"
     CDS             808..1263
                     /locus_tag="PKY13_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT81017.1"
                     /translation="MADTNIDAIDELIIAELQQDGRRPYTQLAKTVGLSEAAIRQRVQ
                     RLLDAGVMQIVAVTDPMRLGYHRAAMVGIKVDGDIRKVAEKLSKFDEVVYVVLTAGTV
                     DLLAEVVVSNDAALLELLNDRIRQIPGVQSTETSIYLNLYKQTYQWGTR"
     gene            1260..2681
                     /locus_tag="PKY13_13580"
     CDS             1260..2681
                     /locus_tag="PKY13_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT81018.1"
                     /translation="MSERPTTRTPEELHELGARHLWMHFSRMGNYAGAGHGGTEQLKV
                     MERGEGCYLYDGDGKRYLDGLSGLFTVNAGHGRPELAAAAAAQASQLAYFPIWGYAHP
                     PAIELAARLTELSPGDLNRVFFTTSGSEAVETAWKLARQYFQAIGEHRRYKVISRNVA
                     YHGTSLGALAITGVASLRTPFEPLAPGGRHVPNTNRYRPFTGGVVGEGGISEEEFGRA
                     AADAIEQCILMEGPESVAAVFLEPLQNAGGCFTPPAGYFARVREICDRYGVLLVSDEV
                     ICAYGRLGHMFGADRYGYVPDIITSAKGLTSGYSPLGAVLVSDRIMEPFLTGEASFAH
                     GITFGGHPVSCAVALANLDLFEHDDLCGHVLAHQDEFRSTLENLRDIGIVGDVRGDGY
                     FWAIELVRDQATRETFTHQEAEDLLRGYLSPRLFDDGLICRADDRGEPVLQFSPPLTA
                     GPEEFDLIGTVLRGALSDAAARL"
     gene            complement(2678..3160)
                     /locus_tag="PKY13_13585"
     CDS             complement(2678..3160)
                     /locus_tag="PKY13_13585"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOT81019.1"
                     /translation="MLHVVLVEPQIPPNTGNVIRLCANVGAALHLVEPLGFDIDDASL
                     RRAGLDYHEFVDMEVHSSLGECLGSLVGRSGDWYGFSRHGTTRYDQVAYGPDDVLVFG
                     TEPTGLSKQHRESFGDRLLSLPMRPDNRSVNLSNSVAIVAYEAWRQAGFPGAAPTGGS
                     "
     gene            3186..4241
                     /locus_tag="PKY13_13590"
     CDS             3186..4241
                     /locus_tag="PKY13_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT81020.1"
                     /translation="MPPVEESPVEAGHEPAPDTVIEVPSGPGTFQRREPALNDAQREI
                     IDRLRSVGVERPRFEPGLRDHLRLEIERGLAPLIEDLGEEDLWLSKHKLEQVHGCEGK
                     YLAEKELDFVWSAPIARGIIAHKAIEIEITSKRKWASLDLIDEAIARFQNEGRGVGEW
                     LQTASEADIDMVRAEANNALCSFQECWPPLKREWRPATEVAMRAEFFTGRIVLSGKPD
                     LTIGYADGSTAGKVIVDFKTGRTAASHVADLRFYALLDALRVGVPPRMLVTYYLDSGT
                     LQAEPVSEEILDATVARTVAAAKRMVSLETGEREPRLAASGACRWCPALPGCSVGRNF
                     LDEQSDAIGDPTADDDL"
     gene            complement(4307..>4834)
                     /locus_tag="PKY13_13595"
     CDS             complement(4307..>4834)
                     /locus_tag="PKY13_13595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT81021.1"
                     /translation="VAGIASPLFSFLPASYGQEVFAVTEWRLGLLQGAGGLGAIVLAP
                     VILTLGARLSRKRLLVVAMTSYGAATALVGVAPVWWVAVIGLALYGGAYLAIASALNT
                     TVQLLAREDMRGKVIAIYLGCLTGALPVGLVLWGWAADAWGIRPVTVVAGLLLVVVTG
                     WFAAAGRFDAMAAAD"
BASE COUNT          798 a         1805 c         1553 g          678 t
ORIGIN      
        1 tcgtgggaga ccgcggtcgc ctgggactca ttgagcccgc gagcccaccg gggcacctcg
       61 gccttctcgg cgacagggtc ggcgtcaacg aacatccgtt cgcaagacta gcccgagcgg
      121 actcgccgat ctgcagcgag tcataccggg cttcgatccc gccgacccca cctgaccgtc
      181 tgccagactg gcccccgtgc atgtgaccgc caaggccgac tacgcgatgc gggcgatcgt
      241 cgagatctcc gtcgctccgg acggacgcgc caaggccgaa cagatcgcct ccgcccagga
      301 catcccgctc ggattcctcc gcggcatcct caccgacctg cgtcgttccg ggctcatcgt
      361 gtcgcagcgg agcgccaacg gcggcttccg cctggcccgc cctgcgtcgg agatcaccat
      421 cgccgaggtg gtccgctccg tcgagggtcc gctggcgctc gttcgtggcc tccgtccgac
      481 cgaactcgac tacccggggg cgacggcacc gctgcgtgac gtgtggatcg cactccgggc
      541 caacctccgg gcggtgctcg accacaccac gatcgccgac gtcgccgagg gccgcctgcc
      601 cgaactcgtc aggtccctgg ccgccgaccc cgactcctgg ctgtaggcaa acggtccact
      661 caccgccgcg gcgacccacc gctgcgaata ccaccggtcc gaggggatcg gaactgcgaa
      721 tcacgcaccc agaacgaccc ctgactacga tcagcgatgg cgtccgccgc gtccggcgac
      781 accgaacctg acacgaacgg gggccggatg gccgacacga acatcgacgc catcgacgag
      841 ctcatcatcg ccgagctcca gcaggacggg cggcgtccct acacccagct cgccaagacg
      901 gtcgggttgt ccgaggcggc gatccgccaa cgcgtccagc gcctcctcga cgccggcgtc
      961 atgcagatcg ttgcggtcac cgaccccatg cgcctcggct accaccgcgc cgcgatggtc
     1021 ggcatcaagg tcgacggcga catccgcaag gtggccgaga agctctcgaa gttcgacgag
     1081 gtcgtctacg tggtcctgac cgccggcacc gtcgacctcc tcgccgaggt cgtcgtgtcg
     1141 aacgacgcgg cgctgctcga gctcctcaac gaccgcatcc gtcagatccc cggcgtccag
     1201 agcaccgaga cgtcgatcta cctcaacctc tacaagcaga cctaccaatg gggaacccga
     1261 tgagcgaacg acccaccacg agaacgcccg aggagctgca cgagctcggc gcacgccacc
     1321 tctggatgca cttcagccgc atgggcaact acgccggcgc cggccacggc ggcaccgagc
     1381 agctcaaggt gatggaacgc ggcgagggct gctacctcta cgacggcgac ggcaagcgct
     1441 acctcgacgg cctgtcggga ctgttcaccg tcaacgccgg ccacggtcga cccgagctcg
     1501 ccgctgccgc cgcggcgcag gcgagccagc tcgcctactt ccccatctgg ggctacgccc
     1561 acccgccggc gatcgagctc gccgcccgtc tcaccgaatt gtcccccggc gatctcaacc
     1621 gcgtgttctt cacgacgtcg gggtccgagg ccgtcgagac ggcatggaag ctggcccgcc
     1681 agtacttcca ggcgatcggc gaacaccgcc gctacaaggt catcagccgt aacgtcgcct
     1741 accacggcac ctcgctgggg gccctcgcca tcaccggtgt cgcgtcgttg cgcaccccgt
     1801 tcgaacccct ggctcccggg ggccgccacg tgccgaacac caatcgatac cgccccttca
     1861 ccggtggcgt cgtcggcgag ggcggcatct ccgaggagga attcggccgg gcggccgcgg
     1921 acgccatcga gcagtgcatc ctcatggagg ggccggagtc ggtggccgcc gtcttcctcg
     1981 aaccgctcca gaacgccggc ggttgcttca cgccgcccgc cggctacttc gcccgggtgc
     2041 gcgagatctg cgaccgctac ggagtgctgc tcgtgagcga cgaggtcatc tgcgcgtacg
     2101 gccgcctcgg ccacatgttc ggcgccgatc gttacggcta cgtgcccgac atcatcacgt
     2161 cggccaaggg cctgacgtcg ggctactcgc ccctcggcgc cgtgttggtg tccgatcgga
     2221 tcatggagcc gtttctcacg ggcgaggcat cgttcgccca cggcatcacg ttcggcggtc
     2281 accccgtcag ctgtgccgtg gccctcgcca acctcgacct gttcgaacac gacgacctgt
     2341 gcggccacgt gctcgcccat caggacgagt tccggtcgac actcgagaac ctgcgcgaca
     2401 tcggaatcgt cggcgacgtt cgcggcgacg gctacttctg ggcgatcgag ctcgttcgcg
     2461 accaggccac ccgtgagacg ttcacccacc aggaggccga agacctgctg cggggctacc
     2521 tgtcgccccg gctgttcgat gacgggctga tctgccgcgc cgacgaccgc ggcgagccgg
     2581 tgttgcagtt ctccccgccg ctcaccgccg gccccgagga gttcgacctg atcggcacgg
     2641 tgctccgcgg cgcgctgtcc gacgcggccg cccggctcta gctgcccccg gtcggcgcgg
     2701 cgcccgggaa ccctgcctga cgccacgcct cgtacgccac gatcgccacc gagttcgaca
     2761 ggttgacgct gcgattgtcg ggacgcatcg gcagcgacag caagcgatcg ccgaacgact
     2821 cgcggtgctg cttcgacagg cccgtcggct cggtgccgaa caccagcacg tcgtcgggcc
     2881 cgtacgcgac ctggtcgtaa cgggtggtcc cgtgacggct gaacccgtac cagtcgccgc
     2941 tgcgaccgac gagcgaaccc aggcactccc ccagcgacga gtgcacctcc atgtccacga
     3001 actcgtggta gtcgaggccg gcgcgtcgca gtgacgcgtc gtcgatgtcg aagcccaacg
     3061 gctcgacgag gtgcagggca gcaccgacgt tcgcgcacag gcggatgacg ttgccggtgt
     3121 tgggagggat ctgcggttcg acgagcacga cgtggagcat cagggttgct ctctagtatc
     3181 ggcccatgcc ccctgtcgag gagagtccgg tcgaggcggg ccacgagccg gctcccgaca
     3241 ccgtcatcga ggtcccgtcc gggcccggca cgttccagcg ccgagaaccg gcgctcaacg
     3301 acgcccaacg cgagatcatc gatcgcctgc gatccgtcgg ggtggagcgg ccccgcttcg
     3361 agcccggcct ccgcgaccac ctccgcctcg agatcgaacg cggactcgcg ccactcatcg
     3421 aggacctcgg cgaagaagac ctgtggctct cgaagcacaa gctcgagcag gtgcacgggt
     3481 gcgagggcaa gtacctcgcc gagaaggagc tcgacttcgt gtggagcgct ccgatcgccc
     3541 gcggcatcat cgcccacaag gcgatcgaga tcgagatcac ctccaaacgg aagtgggcgt
     3601 cgctcgacct gatcgacgag gcgatcgccc ggttccagaa cgagggccgc ggcgtcggcg
     3661 agtggctgca gacggcgagc gaagccgaca tcgacatggt tcgcgccgag gccaacaacg
     3721 cgctgtgcag cttccaggag tgctggcctc cgttgaagcg ggagtggcgc cccgccaccg
     3781 aggtcgcgat gcgcgccgag ttcttcacgg gccgcatcgt gctgtcgggc aagcccgatc
     3841 tcaccatcgg ttacgccgac ggctcgaccg ccggcaaggt catcgtcgat ttcaagacgg
     3901 gccgcaccgc ggcgagccac gtcgccgacc tgcgcttcta cgcgctcctc gacgcgctgc
     3961 gcgtcggcgt gccgccgcgc atgctggtca cctactacct cgactccggc accctgcagg
     4021 ccgaaccggt cagcgaggag atcctcgacg ccaccgtggc ccgcaccgtc gccgcggcca
     4081 aacgcatggt ctcactcgag acgggcgaac gcgagccccg gctcgccgcg tcgggcgcct
     4141 gccggtggtg ccccgcactc cccggctgtt cggtcgggcg caacttcctc gacgaacaga
     4201 gcgacgccat cggcgatccc accgccgacg acgacctcta gtccgaaggt tctgccctgc
     4261 agttcgatcg gcaaaccgca cgaattgcag ggcagaacgg ctggggtcag tcggcggcgg
     4321 ccatcgcgtc gaaccgcccg gcggcggcga accagccggt caccaccacg aggagcagcc
     4381 cggccacgac ggtcacggga cgaatgcccc acgcatcggc ggcccagccc cagaggacga
     4441 gtccgacggg cagcgccccg gtgaggcagc cgaggtagat ggcgatgacc ttgccgcgca
     4501 tgtcctcccg ggcgagcagc tgcacggtcg tgttcagcgc cgaggcgatc gcgaggtacg
     4561 caccgccgta gagcgcgaga ccgatcaccg ccacccacca caccggcgcc acgcccacca
     4621 gcgccgtcgc cgccccgtag gacgtcatgg ccacgaccag cagtcgcttt cggctcaggc
     4681 gggcgcccag cgtgaggatc accggggcca gcacgatcgc gccgaggccg ccggcgccct
     4741 gcagcaggcc gagccgccac tcggtgaccg cgaacacttc ctggccgtag ctcgcgggca
     4801 ggaacgagaa cagcggactg gcgatgcccg ccac
//
LOCUS       DAOSOA010000263         4832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17640_length_4832_cov_6.363199, whole genome shotgun sequence.
ACCESSION   DAOSOA010000263 DAOSOA010000000
VERSION     DAOSOA010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17640_length_4832_cov_6.363199"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..1103)
                     /locus_tag="PKY13_13600"
     CDS             complement(243..1103)
                     /locus_tag="PKY13_13600"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT81022.1"
                     /translation="MPNIFTDTTPVVDPSDPNSPTCVITDAWGLVYAQGGVVMAAALR
                     AIETVLDRPDLTLASASATFCRPVACGPVTTEVEVLRSGRRGAQVHATLRDQHSTDGG
                     ANVVLTAVFTDPELEGPRLSPLERPVELSEPPGDVVESAVAADDRFELGFLDNTVWHL
                     AATPAHADAPVVAGSPVPRLALWFRFTDPPAPLDVDWAPALLPIPGDSLGSALVVALG
                     GGDTPVGSVSLQIGIQVLAPISGRWIGIDSLCTDVGNGLASGVVHLWSESGAHVATVT
                     QTAMLRSFGG"
     gene            1334..2377
                     /locus_tag="PKY13_13605"
     CDS             1334..2377
                     /locus_tag="PKY13_13605"
                     /inference="COORDINATES: protein motif:HMM:NF023425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HOT81023.1"
                     /translation="MPRTRTSHASTNAPRRHRSARPRLALVGVAVVAPTLWFALPAAA
                     AVAPIGLGTASSFSVLAGAGITNTGPTTMWGDAGTFATTSESGFGTVTILGTNHGGDA
                     VTQQAKNDLTTAYNQAAASAPPTNVADELGGLTLTPGVYDGAALQLTGTLTLDTLGDP
                     NAVFVFRTGSTLITASSSDVIVLGGGVACNVFWQVPSSATLGTASHLIGTVMASTSIT
                     ANTGATIVGRLLASTGAVTLDSNTLTNTGCADSTMSTTTSTTTSTTPPTTAPPTTAAA
                     VPTSSTTPTGSSTPTTTPGDITQGERPPLAFTGPGRSVPFAVTAGVSLFVGTAALRIR
                     RHRLRAAGSPGSA"
     gene            complement(2369..2980)
                     /locus_tag="PKY13_13610"
     CDS             complement(2369..2980)
                     /locus_tag="PKY13_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT81024.1"
                     /translation="MCTVLDVQQRAGHHPDMRERILDAAEACLRRDGIRRTTVAAVAA
                     EADISRAYVYRFFADKPTLVSAALIRRDEAFWAEADQRVAAADGLAGMVAEAVLLSRA
                     APLGSLALQLAETEPAEYAAVMGTFVHEIVPGLSGFWTDQLRRAQARGVIDEGVDLDA
                     GAEWVIRAVVSLVGIPGRAVDVDDRASLVDFLEAFLTPAFRSG"
     gene            3054..4754
                     /locus_tag="PKY13_13615"
     CDS             3054..4754
                     /locus_tag="PKY13_13615"
                     /inference="COORDINATES: protein motif:HMM:NF023500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HOT81025.1"
                     /translation="MGFLQPNLPVVDHEEWGRLPRQERLAPMARHFAEFGFGSPDVVI
                     LFYLFKIVLYVAGGWAFVLTTPGIDGFFDPGSWWTRPEVFFKFALWTSLFEVLGLGCG
                     FGPLNLRFVPPMGSFLYWLRPGTIRLPAGNGRLPLTRGDTRTVVDAVLYAGLLVSLVV
                     GLWGALTRTEVVVALAFLALIGLRDQTIFLAARGEVYGTLLLAYLFAAGDQITAAKLV
                     LVVIWWGAAASKINQHFPFVVSAMLSNSPLWRFGNVKRRLHRNFPDDLRPGRIPTLLA
                     HGGTVVEFAAPAVMLVTSGWPLYIAATVMIGFHLIILTSFPMGVPLEWNVFMIGGIVT
                     MFLGHEGLHLGDLRQPALVGLLIVALVAMVVYGNLVPRNVSFLLSMRYYAGNWAATFW
                     CFRGDALERFDANVVKASMLPHQQLEQVYGSAEEAAVPLHMGYAFRGFHTHGRALWTL
                     VPRACGPEHEDYLVLDGELVAGTALGWNFGDGHLHDEQLIAALQSRCDFRPGDVRVVF
                     LESQPFHRRTQEYRLIDAATGEIERGWVDVNDLVEAQPTDANVPVHLHPQASGREIAR
                     "
BASE COUNT          733 a         1815 c         1568 g          716 t
ORIGIN      
        1 gcagtacgcc gctggtcgtc ccgccggttc gccgtcggcg ctcgccaccg aggcgccctc
       61 gaagccgagc ccgatcaggc ctgccaccac tgcgatcgcc agcgcgatcc tgctccgtcg
      121 tccccgcacg ttccctcccc agatgaaccg acgaggaacg taacaaatag tatggcagtc
      181 ggcaactgtc cgaacctgaa cgaagctgcc cgaactggga ctggcccgcg gggtcggcag
      241 ggtcagccgc cgaaggaacg gagcatcgcg gtctgcgtca cggtcgcgac gtgagctccc
      301 gactcgctcc acaggtgcac cacaccgctc gcgagcccgt tgcccacgtc ggtgcacaac
      361 gagtcgatgc cgatccatcg gccgctgatc ggggcgagga cctggatgcc gatctgcagc
      421 gacacgctgc cgacgggggt gtcgccgccg ccgagggcca cgacgagcgc cgagcccaac
      481 gagtcgccgg ggatcgggag cagggccggg gcccagtcga cgtcgagcgg cgcgggcggg
      541 tcggtgaagc ggaaccacag cgccagccgc ggcaccgggc tgccggcgac gacgggcgcg
      601 tcggcgtggg ccggcgtcgc ggcgaggtgc cacacggtgt tgtcgaggaa cccgagctcg
      661 aaccggtcgt cggccgccac cgccgactcg acgacgtcgc cgggcggctc gctcagctcc
      721 accggacgtt cgagtggcga caacctcggg ccctccagct cgggatcggt gaacaccgcg
      781 gtgagcacaa cgttcgcccc gccgtcggtc gagtgctgat cacggagcgt cgcgtggacc
      841 tgagcgcccc gacggccgct gcgcagcacc tcgacctcgg tcgtgaccgg cccgcacgcc
      901 accggccgac agaacgtcgc actggcgctc gcgagcgtga ggtccggacg gtcgagcacc
      961 gtctcgatgg cccgcagggc cgccgccatc accacaccgc cctgggcgta cacgaggccc
     1021 cacgcgtcgg tgatcacaca cgtgggcgag ttcggatcgg atggatcgac gaccggggtc
     1081 gtgtccgtga agatgttcgg caccgcatcg tcccttcgct cgcggtcggt acgacgaact
     1141 tactggacag gtgtccagga aaggatcggg ggatcgaggt gatctgtcac ggtcggtgtt
     1201 ctcaagcatc ggcccggcac cgccgatact ccgactacgc cccgagaggg cacacctggt
     1261 tcgaacgcac agcctcccgc tcggtgcatg gtcgacggac acgacgcgac ctctccacct
     1321 cggaagtagc gctatgcctc gaacacgaac cagccacgcc agcacgaacg cgccccggcg
     1381 ccatcggtcg gcgcgtcccc gtcttgccct cgtcggcgtc gccgtcgtgg cccccacgct
     1441 ctggttcgcc cttcccgccg ccgctgccgt cgcaccgatc ggcctcggga cggcgtcgtc
     1501 gttctcggtc ctcgccgggg ccggcatcac caacaccggg cccacgacga tgtggggcga
     1561 cgccggcacg ttcgccacca ccagcgagag cggcttcggc accgtcacga tcctcgggac
     1621 gaaccacggc ggcgatgccg tcacccagca ggccaagaac gacctcacca ccgcgtacaa
     1681 ccaggcggca gcgagtgcac cgcccacgaa tgtcgccgac gagctcggcg gactgaccct
     1741 gacacccggc gtgtacgacg gcgcagcgct gcagctcacc ggcacgctca cgctcgacac
     1801 cctgggcgac cccaacgccg tgttcgtctt ccggacgggc tcgacgctca tcaccgcgag
     1861 ctcgagtgac gtgatcgtgc tcggcggggg cgtcgcctgc aacgtgttct ggcaggtgcc
     1921 cagctcggcc accctcggga ccgcgtcgca cctgatcggc acggtgatgg cctcgacgtc
     1981 catcacggcg aacaccggcg ccaccatcgt gggccgactc ctcgcctcga caggagcagt
     2041 caccctcgac agcaacaccc tgaccaacac cgggtgcgcc gactcgacga tgagcaccac
     2101 gacgtcgacc acgacgtcga ccacaccgcc gacgacagcc ccaccgacca ccgcggccgc
     2161 cgtgcccacg agctcgacga cgccgacagg gtcgtcgaca cccacgacga cgcccggcga
     2221 catcacccag ggtgagcgac ccccgttggc cttcaccggc cccggtcgct ccgtcccgtt
     2281 cgccgtgact gcgggggtgt cgctgttcgt cggcacggcc gcgctgcgga tccgtcgcca
     2341 ccgcctgcga gccgccggat cgcccggttc agcctgaccg gaacgccggc gtgaggaacg
     2401 cttcgaggaa gtcgacgagc gacgcccggt cgtcgacgtc caccgccctc cccgggatac
     2461 cgacgaggga cacgaccgcc cggatcaccc actcggcgcc ggcgtcgagg tcgacgccct
     2521 cgtcgatgac cccccttgcc tgcgcccgac gcagctgatc ggtccagaac cccgagagtc
     2581 cgggcacgat ctcgtgcacg aacgtgccca tgaccgctgc gtactcggcc ggctcggtct
     2641 cggcgagctg cagtgccagc gagccgaggg gcgccgcccg cgacaggagc acggcctcgg
     2701 cgaccatgcc ggcaaggccg tccgcggccg ccacacgctg atcggcctcg gcccagaagg
     2761 cctcgtcacg acggatcaac gctgccgaca cgagggtcgg cttgtcggcg aagaaccggt
     2821 acacgtaggc gcgcgagatg tcggcctcgg ccgcgaccgc agcgaccgtg gttcgccgga
     2881 tgccgtcgcg acgcaggcag gcctcggcgg catcgaggat ccgttcccgc atgtcggggt
     2941 gatgccccgc ccgctgctgc acgtcgagca cagtacacac tttgtgggaa gtgtgtactg
     3001 tggctcccgc accgggccgc aacggttccg gcgctcgtcg agggggacac gcgatgggat
     3061 tcctgcagcc caacctgccg gtcgtcgatc acgaggagtg gggccggctc ccccgccagg
     3121 aacggctggc gcccatggcc cggcacttcg ccgagttcgg cttcggatca cccgacgtcg
     3181 tcatcctctt ctacctgttc aagatcgtgc tctatgtggc cggcggatgg gcgttcgtgc
     3241 tcacgacgcc gggcatcgac gggttcttcg atccgggctc gtggtggacc cgacccgagg
     3301 tgttcttcaa gttcgcactc tggacgtcgc tgttcgaggt gctcggcctc ggatgcgggt
     3361 tcgggccgct caacctgcgc ttcgttccac cgatgggctc gttcctctac tggctgcggc
     3421 ccggcaccat ccgcctgccc gccggcaacg gtcgtctccc gctcacccgg ggcgacaccc
     3481 gtacggtcgt cgacgccgtg ctctacgccg ggctcctggt ctcgctcgtc gtcggtctct
     3541 ggggcgcgct gacccgcacc gaggtcgtgg tcgcgctggc gttcctcgca ctcatcgggc
     3601 tccgcgacca gacgatcttc ctcgcggcac gcggcgaggt ctacggcacg ctcctgctcg
     3661 cctacctgtt cgccgccggc gatcagatca ccgctgccaa gctcgtcctc gtcgtcatct
     3721 ggtggggtgc cgccgcctcg aagatcaacc agcacttccc cttcgtcgtc tccgcgatgc
     3781 tcagcaacag cccgctctgg cggttcggca acgtcaagcg gcgcctgcac cgcaacttcc
     3841 ctgacgacct ccgccccggc cgcatcccca ccctcctcgc ccacggcggc accgtcgtcg
     3901 agttcgctgc gccggcggtg atgctcgtca cgagcggctg gccgctctac atcgccgcca
     3961 ccgtgatgat cggcttccac ctgatcatcc tcaccagctt cccgatgggc gtcccgctcg
     4021 aatggaacgt gttcatgatc gggggcatcg tcacgatgtt cctcggccac gaggggctcc
     4081 acctcggcga cctccggcag ccggcgttgg tggggttgct catcgtggcg ctcgtcgcga
     4141 tggtcgtcta cggcaacctc gtcccccgca acgtgtcgtt cctgctgtcc atgcggtact
     4201 acgccggcaa ctgggccgcc acgttctggt gcttccgtgg cgacgccctc gaacgcttcg
     4261 acgccaacgt cgtgaaggcg tcgatgctcc cccaccagca gctcgagcag gtgtacggca
     4321 gcgccgagga agccgccgtg ccgctgcaca tggggtacgc gttccgtggg ttccacaccc
     4381 acggccgggc gctgtggacc ctcgtgccga gggcgtgcgg acccgagcac gaggactacc
     4441 tggtgctcga cggcgagctc gtggccggca ccgccctcgg atggaacttc ggcgacggac
     4501 acctccacga cgaacagctg atcgccgcgt tgcagtcacg ctgcgacttc cggcccggcg
     4561 atgtccgggt cgtgttcctc gagtcccagc cgttccaccg ccgcacccag gagtaccgcc
     4621 tcatcgacgc cgcgaccggc gagatcgagc gcgggtgggt cgacgtgaac gacctcgtcg
     4681 aggctcaacc gaccgacgcg aacgtgccgg tgcacctgca cccccaggct tccggtcgcg
     4741 agatcgcccg ctgagcgggc gacaacgcga ccagaaccgg agggattcgg gtcagaggcc
     4801 gaggggatcg tccacgaggc cgagccacac ct
//
LOCUS       DAOSOA010000264         4808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17739_length_4808_cov_5.219861, whole genome shotgun sequence.
ACCESSION   DAOSOA010000264 DAOSOA010000000
VERSION     DAOSOA010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17739_length_4808_cov_5.219861"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKY13_13620"
     CDS             complement(<1..646)
                     /locus_tag="PKY13_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81026.1"
                     /translation="MRATYARSLADVLHRCSDRTARRRVTALLLSAVLVTACSGNGLD
                     DAARSTTTTTDVIGRDRALPIASDSLGGWKAPELEWSGCTGERGFECATLTVPLDWDD
                     VDGDTIDLALGRDQASGDRIGALVTNPGGPGGSGLDFLFSQPFDETLSSRFDIVSWDP
                     RGVGRSTAFACEQHVDDFLANDPDPDTAAEQAAIDEDAETVADDCATSSPGLAAN"
     gene            645..1595
                     /locus_tag="PKY13_13625"
     CDS             645..1595
                     /locus_tag="PKY13_13625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT81027.1"
                     /translation="MIFGTFIPQGWKMELASIPDPQDKWAATVHIATEAERFGYDSVW
                     VYDHFHNVPVPAHEAMFECWTTLAAISQRTERVRLGQMVSCAPYRNPGMIAKITSSID
                     VISGGRLDWGIGAGWYDGEFTAYGYDFPSAADRIRALRETVEIVKLMWSEPDATYEGR
                     HHSVTGAQCDPKPLQSPHPPIWIGGGGEQLTLRVVARHATHSNWGGTPEVWAHKAEVL
                     KGHCEAIGRDYDTITKTWSPELFVRSTEAEIGTESRSFWGEPFETWSTNNLVGTPEQV
                     AEKLEAYRKLGVGGVVPWIADFPSTETLELFATEVMPNFR"
     gene            complement(1677..2135)
                     /locus_tag="PKY13_13630"
     CDS             complement(1677..2135)
                     /locus_tag="PKY13_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOT81028.1"
                     /translation="MATQINGIAGMKELVGQHLGYSDWLEITQERVNQFAEATGDHQW
                     IHVDPERAKDGPFGGTIAHGYLTLSIGPMLAPQVMQATGFKMGVNYGCGKVRFPSPVP
                     VGSKLRLGVEIKEIDELAPGQAQCTYLYTFEVEGASKPSCVAEVIVRMYE"
     gene            complement(2192..3103)
                     /locus_tag="PKY13_13635"
     CDS             complement(2192..3103)
                     /locus_tag="PKY13_13635"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT81029.1"
                     /translation="MQRFQRYDDHGWFKVGQVEVTTSVLLAASSLIFMVLGVIAAAIF
                     RDPLYYDATLVREGEVWRVVTWPLSNGVSIWLLLSAAMMYLIGGDIERELGRRRFAWL
                     IGALVVIPAVVTVGFEYVGAAGISRLVSPLFVVLVLWHPTIRTFFNIPLWAVVVVFEV
                     FDLLQILDLRNAGAPIGGLLTFWAASLAVAALGARAFGITEFTQIPKIPLPQFITGDP
                     YQKANRAREKQQRKQSKGSGGSGGGLRRHKDPAPVIPIRPEARLDRADQADMDWLLDK
                     ISASGMDSLTPDERRRLDEHSRRLRGQ"
     gene            complement(3109..4209)
                     /gene="rlmN"
                     /locus_tag="PKY13_13640"
     CDS             complement(3109..4209)
                     /gene="rlmN"
                     /locus_tag="PKY13_13640"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOT81030.1"
                     /translation="MSATAPTRYGIDRSGLADLLADQPRYRVDQIWEGWYRQLRRSDE
                     LTNLPKALRARLDDELPTSLDLVARSVSDDGDTTKFLWRLHDGATVETVLMLYPDRAT
                     VCISTQAGCAMACGFCATGQAGFERHLTVGEVVEQVIAAARVAADATTPRRVSNIVFM
                     GMGEPLANYSATWGAVERLHADVGISARHLTVSTVGIIPGIRRIATEALPINLAVSLH
                     AANDRLRDELVPINKRYPLDALAEACAEYLAVKNRRLSFEWAMIAGVNDRDADADELA
                     AYARSLRAHINLIPLNPTPGYPTVGSSRERVRQFRDRLTDAGANATIRANRGTEIDAA
                     CGQLSTLHGQQAAGSGTVSVELAPKRPATLPD"
     gene            complement(4206..>4808)
                     /gene="mshD"
                     /locus_tag="PKY13_13645"
     CDS             complement(4206..>4808)
                     /gene="mshD"
                     /locus_tag="PKY13_13645"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HOT81031.1"
                     /translation="PSSAAEDTAAHIGMTEHRRLFQMRRPLPLDLTPVDDAEPPPTLD
                     TRAFDPDRDADTWLAINNAAFSWHPEQGGWTRETLEARMAEPWFDLDDFRIADLDGTM
                     AGFCWTKVHPETATEPRLGEIYVIAVAPARVGTGLGTALTVAGLDWLWARRHTPVGML
                     YVESDNDRALATYRRLGFRIHSTDVAFEPPPGAAIGQSAP"
BASE COUNT          727 a         1537 c         1796 g          748 t
ORIGIN      
        1 cgttggcggc gaggccgggc gaggacgtgg cgcagtcgtc cgcgacggtt tcggcgtcct
       61 cgtcgatcgc cgcctgctcg gcggcggtat cggggtcggg gtcgttggcc aggaagtcgt
      121 cgacgtgctg ctcgcaggcg aatgcggtgc tcctgccgac accgcggggg tcccagctga
      181 cgatgtcgaa acggctgctc agcgtctcgt cgaagggctg gctgaagagg aagtcgaggc
      241 cacttccacc cggcccgccg gggttggtga cgagggctcc gatgcggtcg cccgacgcct
      301 gatcgcgccc gagcgcgagg tcgatggtgt cgccgtcgac gtcgtcccag tcgagcggaa
      361 cggtgagcgt ggcgcactcg aacccgcgct cgcccgtgca gcccgaccac tcgagctccg
      421 gtgccttcca acctccgagc gagtcggacg cgatggggag cgcccggtcg cgtccgatca
      481 cgtcggtggt ggtggtggtg gagcgggcgg cgtcgtcgag accgttgccc gagcacgcgg
      541 tgacgaggac cgcggacagg agcagggcgg tgacgcggcg gcgagcagtg cggtcggagc
      601 atcggtggag cacgtcggcc aggctacggg catatgttgc tctcatgatc ttcggcacct
      661 tcatccccca gggttggaaa atggaactcg cgtccatccc cgacccccag gacaagtggg
      721 cggccaccgt ccacatcgcg accgaggccg agcgtttcgg ctacgactcg gtgtgggtct
      781 acgaccactt ccacaacgtc ccggtgccgg cgcacgaggc gatgttcgag tgctggacga
      841 ccctcgccgc catcagccag cgcaccgagc gggtgcggct cggccagatg gtcagctgtg
      901 cgccgtaccg caacccgggc atgatcgcga agatcacgtc gtcgatcgac gtgatcagcg
      961 gcggtcgtct cgactggggc atcggcgccg gttggtacga cggcgagttc acggcctacg
     1021 gctacgactt tccgtccgcc gccgaccgga tccgcgccct gcgcgagacc gtcgagatcg
     1081 tgaagctcat gtggagcgag cccgacgcga cctacgaggg ccggcaccac tcggtgaccg
     1141 gcgctcagtg cgatccgaag ccgctccagt caccgcatcc acccatctgg atcggcggcg
     1201 ggggcgagca gctcacgctg cgcgtcgtcg cccgccacgc gacccactcg aactggggcg
     1261 gcacgcccga ggtgtgggcg cacaaggccg aggtgctcaa agggcattgc gaggcgatcg
     1321 gccgcgacta cgacacgatc accaagacgt ggtcgccgga gctgttcgtg cgctcgaccg
     1381 aggccgagat cggcaccgag tcccgcagct tctggggcga gccgttcgag acgtggagca
     1441 ccaacaacct cgttggcacc cccgagcagg tggccgagaa gctcgaggcc taccgcaagc
     1501 tcggcgtcgg tggtgtggtg ccgtggattg ccgacttccc gtcgacggag accctggagc
     1561 tgttcgccac cgaggtcatg cccaacttcc gctgagcacg cgccaccggt tctgtcctgc
     1621 agttctgccc ctggaggggc gaatctgcag gacagaaccg gtgaggcgct gggttcctac
     1681 tcgtacatcc ggacgatgac ctcggcgacg cagctcggct tcgacgcacc ctcgacctcg
     1741 aaggtgtaga ggtacgtgca ctgggcctga ccgggggcga gttcgtcgat ctccttgatc
     1801 tcgacaccga gacggagctt cgagccgacg ggcacggggc tcgggaagcg caccttgccg
     1861 cagccgtagt tgacgcccat cttgaagccg gtggcctgca tgacctgcgg ggcgagcatc
     1921 gggccgatgc tcagcgtgag gtagccgtgg gcgatggtgc cgccgaaggg accgtccttg
     1981 gcgcgctcgg gatcgacgtg gatccactgg tggtcaccgg tcgcctcggc gaactggttc
     2041 acccgctcct gggtgatctc cagccagtcg ctgtagccaa ggtgctgtcc gacgagttcc
     2101 ttcatgccgg cgatgccgtt gatctgagta gccatggcgg cgattcttgc tcagcgcggc
     2161 ggtggtcggc gaatgaaccg ccggtgccgg gttactggcc gcggaggcgg cggctgtgct
     2221 cgtcgaggcg acggcgctcg tcgggcgtga gactgtccat gcccgaggcg ctgatcttgt
     2281 cgagcagcca gtccatgtcg gcctggtcgg cgcggtcgag gcgcgcctcg ggacggatcg
     2341 ggatgaccgg cgccgggtcc ttgtgccgcc gcaacccgcc gccggaacca cccgagccct
     2401 tcgactgctt gcgctgctgc ttctcgcggg cgcggttggc cttctggtac gggtcgccgg
     2461 tgatgaactg ggggagcggg atcttgggga tctgggtgaa ctcggtgatg ccgaaggcgc
     2521 gggccccgag ggcggcgacg gccagtgagg ccgcccagaa ggtgagcagg ccgccgatgg
     2581 gggcgccggc atttcggaga tcgaggatct gcaggaggtc gaagacctcg aacaccacca
     2641 cgaccgccca gagagggatg ttgaagaacg tccggatcgt ggggtgccac agcacgagca
     2701 ccacgaagag cgggctgacg aggcggctga tgccggcggc cccgacgtat tcgaaaccga
     2761 cggtgacgac cgcagggatc accacgagcg cgccgatcag ccaggcgaac cgccggcggc
     2821 cgagctcgcg ctcgatgtcg ccgccgatca ggtacatcat cgcggcggac aggagcagcc
     2881 agatactgac gccgttgctg agcggccacg tgacgacgcg ccacacctcg ccctcgcgga
     2941 ccagcgtcgc gtcgtagtag agcgggtccc ggaagatggc cgcggcgatg acaccgagga
     3001 ccatgaagat gagtgacgac gcggcgagga ggaccgaggt cgtgacctcg acctggccca
     3061 ccttgaacca gccgtggtcg tcgtagcgtt ggaaccgttg cacggcgatc agtctggcag
     3121 cgtcgccggg cgtttcggtg ccaactccac cgacacggtc cccgaacccg ctgcctgctg
     3181 cccgtggagt gtgctcagct ggccgcacgc ggcgtcgatc tcggtgccgc ggttggcgcg
     3241 gatggtggcg ttggcgccgg cgtcggtgag gcggtcccgg aactgccgga cccgttctcg
     3301 ggacgagccg acggtggggt agccgggcgt cgggttgagg gggatgaggt tgatgtgcgc
     3361 ccgcagcgac cgggcgtacg ccgcgagttc atcggcatcg gcgtcgcggt cgttgacccc
     3421 ggcgatcatc gcccactcga agctgagccg gcggttcttg acggcgaggt actccgcgca
     3481 cgcctcggcg agggcatcga gggggtagcg cttgttgatc gggacgagct cgtcgcggag
     3541 gcggtcgttg gcggcgtgca acgacaccgc gaggttgatc gggagcgcct cggtggcgat
     3601 gcgccggatg ccggggatga tgccgaccgt cgacacggtg aggtggcgcg ccgagatgcc
     3661 gacgtcggcg tgcaaccgct cgacggctcc ccacgtcgcc gagtagttcg cgagcggttc
     3721 gcccatgccc atgaacacga tgttcgacac tcgccggggc gtcgtcgcgt cggcggcgac
     3781 ccgggccgct gcgatgacct gttcgacgac ctcgccgacc gtgaggtgtc gttcgaaccc
     3841 ggcctggccg gtggcgcaga agccgcaggc catggcacac ccggcctggg tcgagatgca
     3901 caccgtggcc cggtcgggat agagcatcag gaccgtctcg acggtggcgc cgtcatggag
     3961 gcgccacagg aacttcgtgg tgtcgccgtc gtcgctcacc gaacgggcga cgaggtcgag
     4021 cgaggtgggg agttcgtcgt cgaggcgggc gcgcagggcc ttcggcaggt tggtcagttc
     4081 gtcggagcgg cgcagctggc ggtaccagcc ctcccagatc tggtcgacgc ggtaccgcgg
     4141 ttggtcggcg aggaggtcgg cgaggccgga ccggtcgatg ccgtagcgag tgggggctgt
     4201 ggcgctcacg gggctgattg cccgatggcc gcgcccgggg gcggctcgaa cgcgacgtcg
     4261 gtcgagtgga tgcggaaccc gagccggcgg taggtcgcca acgcccgatc gttgtcggac
     4321 tcgacgtaca acatgccgac cggggtgtgt cggcgggccc acagccagtc gagaccggcg
     4381 acggtgagcg cggtgccgag gcccgtcccg acccgtgccg gagcgacggc gatcacatag
     4441 atctcgccca accgcggttc ggttgccgtc tcgggatgga ccttcgtcca gcagaacccg
     4501 gccatggtcc cgtcgagatc ggcgatgcgg aagtcgtcga ggtcgaacca cggctcggcc
     4561 attcgagcct cgagggtctc gcgggtccag ccgccctgtt cggggtgcca cgagaaggcg
     4621 gcattgttga tcgcgagcca cgtatcggcg tcccgatccg gatcgaacgc ccgggtgtcg
     4681 agcgtggggg gcggctcggc gtcgtcgacc ggtgtgaggt cgagggggag cgggcgtcgc
     4741 atctggaaga ggcggcggtg ctcggtcatg ccgatgtgcg ctgcggtgtc ctcggccgcc
     4801 gacgacgg
//
LOCUS       DAOSOA010000265         4783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17851_length_4783_cov_3.084814, whole genome shotgun sequence.
ACCESSION   DAOSOA010000265 DAOSOA010000000
VERSION     DAOSOA010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17851_length_4783_cov_3.084814"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKY13_13650"
     CDS             <1..703
                     /locus_tag="PKY13_13650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT81032.1"
                     /translation="GVIKMLDANGIHPVVVAGSDLARNYRDATRLLDVSYSLVDESDI
                     VARRSGPWAPEARVLSVGRLDPEKNPLLLADITAGLRDAWTGWRVRAVGAGSLENDLT
                     ARRHALGVDDRLELGGPVPYGDAIREEYAEADVLVHVSHTEGQPQVLLEAMAAGLPIV
                     GTAVGGVPSLLGDGERGLLVGPDDADAIVAGLERLRHEPELRNRLVAAGRDFVRTRTT
                     DVEAGRVVDFIEAVR"
     gene            700..2184
                     /locus_tag="PKY13_13655"
     CDS             700..2184
                     /locus_tag="PKY13_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOT81033.1"
                     /translation="MTDQLSEPVSDLGMGTVARRSMWLSVGSLVGSALAVLTSFVLAR
                     WLGPSRFGRVQGVLLVYFYVALVRTGAFESALRRYIRLRALGRDDEARRARDVGTTVD
                     FVFSLVPALGLVVWGLASDGGLWQRGLLLAPLVVMTTTAGAYLGELRAADHRFDLVAV
                     SGVVRSLSYAALALGGVALFGDTALFVAPALADLLAVAVLASQRPRIGLRPRFDLPVA
                     KDLVRSGLALGALPAVYWMYRLAGSTSVALGTDVVTFGLFSFAAAPVAVLLRAISQVD
                     AVLTPAIWGEIARDEGDRRWLPAADRVTTTLFVVAGFATNLSQAGFGPVVLRFLPDYA
                     DAVPLFEVLAFNVVLLSVGVVPSLVLRDEEHGSQNRYLLLWVGALVVNVAANAVALAA
                     GFGATTVAWNDVWVQAVVVIALFAMAAQRLDVPGWGRRAAIGFPVAAATVATMFGLRL
                     LDGLAVRCVLVLGVWVSILFLVRHRLRPSEEMGRSTHVGAVVAD"
     gene            2233..3210
                     /gene="pseB"
                     /locus_tag="PKY13_13660"
     CDS             2233..3210
                     /gene="pseB"
                     /locus_tag="PKY13_13660"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HOT81034.1"
                     /translation="MALDLNGQTVLVTGGTGSFGTAFTARMLKEFPDIVLRIFSRDEL
                     KQSEMATKFDHDHRLRFFVGDVRSRERLSRATKGVDYIVHAAAMKQVPACEYNPFEAI
                     QTNVLGSQHIVDAAIDNGVKTVVALSTDKAVNPVNLYGATKLCEEKLIVQGNAYAAGS
                     DAKLCCVRYGNVVGSRGSVVPVFREQIASGQLTITDARMTRFWITLDEAVDLVLYALE
                     HTEGGEVFVPKIPSMKVVDLADAMAQGIPTVEVGIRPGEKLHEALLTEDEARHSVDAG
                     DVYVVLPEHPWWGMGGAWEDATPLPDGFAYTSDNNDDWLTADQLTALLA"
     gene            3207..4295
                     /locus_tag="PKY13_13665"
     CDS             3207..4295
                     /locus_tag="PKY13_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT81035.1"
                     /translation="MIPYGRQTIEEDDIAAVVAVLRSDLLTQGPAVEEFEARLCEITG
                     AQYAVAVSSGTAALHAALHAGGITASHTVATSPLSFAASANAARYLGAGVSLIDIEAD
                     TLNLDPTAVPEHAEALVAVHYAGLPVDLGRLNRRPRVIVEDAAHAIGAATPDGPVGNC
                     ARSDMCCFSFHPVKTVTTGEGGAVTTNSPDLADRLRRFRHHGISKNAGDPFWKYDIGD
                     LGYNYRLTDLQAALGTSQLGKLERFIDARQAIAARYRVAFAGDERIDLPPDAPAGFRH
                     AYHLFPIRVDDRDRVFDELRSRGIGVQVHYVPMYRFTDFGGDADGSGVTDYPETEAAF
                     ERLISLPMFPGLTPEDQDIVITTLLEVL"
     gene            4292..>4783
                     /locus_tag="PKY13_13670"
     CDS             4292..>4783
                     /locus_tag="PKY13_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT81036.1"
                     /translation="MTSLDRSREWWARAERVIPLGTQTLSKSPTQFVQGASPIFLEHG
                     RGAHVWDVDGNEFIDYPMALGPVILGYAEPVVNRAIERQLADGITFTLMHPLEVEVAE
                     RIVAMCPGVEAVRFAKTGSDAVSAAVRAARALTSRDAVAVAGHHGWHDWYVGTTTRDL
                     GVPG"
BASE COUNT          672 a         1673 c         1628 g          810 t
ORIGIN      
        1 cggcgtgatc aagatgctgg acgccaacgg catccatccg gtggtggtgg ccggatcgga
       61 tctcgctcgc aactaccgcg acgcgacccg gctcctggac gtgtcctact ccctcgtcga
      121 cgagagcgac atcgtggccc ggcggtcggg cccctgggca cccgaggccc gagtcctgtc
      181 cgtcggtcga ctcgaccccg agaagaaccc gctcctcctc gccgacatca ccgccgggct
      241 ccgggacgcg tggacaggat ggcgggttcg ggccgtggga gcaggaagcc tggagaacga
      301 cctcacggcc cgccgccatg ccctcggcgt ggacgaccgg ttggagctgg gcggaccggt
      361 cccgtacggc gacgcgatcc gtgaggagta cgccgaggcg gacgtgctcg tgcacgtctc
      421 ccacaccgag ggccagccgc aggtgctact cgaagccatg gccgccgggt tgcccatcgt
      481 cggaaccgcg gtcggcggcg tgccctcgct cctgggtgac ggtgagcgtg gcctgctcgt
      541 cggccccgac gatgccgacg ccatcgtggc cggcctcgaa cgacttcgcc acgaaccgga
      601 gctccgcaat cgactggtcg ctgccgggcg cgacttcgtc cggacgagga cgaccgacgt
      661 cgaggccggt cgcgtcgtcg acttcatcga ggcagtgcga tgaccgacca gctgagcgag
      721 ccggtctccg acctcgggat gggcacggtc gctcggcgct cgatgtggct gtcggtgggc
      781 tccctcgtcg gctcggcgct ggcggtcctc acgagcttcg tcctggcccg ttggctcggt
      841 cccagccggt tcgggcgggt gcagggcgta ctgctcgtct acttctacgt ggccctggtc
      901 aggacgggcg ccttcgagag cgcgctgcgc cgctacatcc gactccgtgc gctcggccgc
      961 gacgacgaag cgcgccgtgc ccgcgacgtc ggcaccacgg tggacttcgt gttctcgctc
     1021 gtgccggcgt tgggactcgt ggtgtgggga ctcgcgagcg acggcgggct atggcagcgg
     1081 ggcctgctcc tcgctccgct ggtggtcatg acgaccaccg ccggcgccta cctcggcgag
     1141 ctccgtgccg ccgatcaccg cttcgacctg gtggcggtgt cgggggtggt gcgctccctg
     1201 tcctacgccg cgctagcgct cggtggcgtc gcgctgttcg gcgacacagc gctgttcgtc
     1261 gccccggcgc tcgccgacct actggcggtt gcggtcctcg cgtcgcaacg gccacggatc
     1321 gggttgcggc cccgattcga ccttccggtc gccaaggacc tcgtccgctc cggcctggca
     1381 ctgggggcgc ttccggccgt ctattggatg taccggctcg ccggcagcac gtcggtggcg
     1441 ctcggcaccg acgttgtcac gttcggcctc ttcagtttcg cggcggcacc cgtcgccgtg
     1501 ctcctccgtg ccatcagcca ggtcgacgcc gtgctcacgc cggcgatctg gggcgagatc
     1561 gcccgggacg agggcgatcg acggtggctc ccggccgccg accgcgtcac gaccacgctg
     1621 ttcgtggtcg ccggattcgc gacgaacctg agccaggcgg gcttcggacc ggtcgtgctt
     1681 cggttcctgc ccgactacgc cgacgccgtg cccctcttcg aggtgctggc gttcaacgtc
     1741 gttctgctct cggtgggcgt cgtgccgtcc ctggtcttgc gagacgagga gcacgggtcg
     1801 caaaaccgct acctgttgct ctgggtcggc gcgctcgtgg tcaacgttgc agcgaacgcc
     1861 gtggcgctgg ccgccgggtt cggcgccacg accgtcgcct ggaacgacgt ctgggtacag
     1921 gcagtcgtgg tgatcgccct gttcgcgatg gccgcacagc gcctcgacgt cccgggctgg
     1981 ggtcgccgag cggcaatcgg cttcccggtc gccgccgcca cggtcgccac catgttcggc
     2041 ctgcgcctcc tggacggcct cgccgtccgt tgcgtgctgg tgctcggcgt gtgggtgagc
     2101 atcctgttcc tcgtgcgcca tcgcctccgc ccgtcggagg agatgggccg atcgactcat
     2161 gtcggagccg ttgtcgccga ttgatgacgt agcgtcgtcc gtcgtacgta cggcaccgat
     2221 gaagggatct cgatggcact ggacctgaac ggtcagacgg ttctcgtaac ggggggcact
     2281 ggctccttcg gtacggcgtt cacggcacgg atgctgaagg agttccccga catcgtcctg
     2341 cggatcttct cccgcgacga gctcaagcag tccgagatgg ccaccaaatt cgaccacgac
     2401 caccgtctgc gcttcttcgt cggtgacgtc cgcagtcgag agcggctgag tcgcgccacc
     2461 aagggcgtcg actacatcgt ccacgcagcc gccatgaagc aggtgcccgc ctgtgagtac
     2521 aacccgttcg aggcgatcca gaccaacgtg ctgggctccc agcacatcgt cgacgctgcg
     2581 atcgacaatg gcgtgaagac cgtggtggcg ctgtcgaccg acaaggccgt caaccccgtc
     2641 aatctctacg gtgccacgaa gctgtgcgag gagaagctga tcgttcaggg caacgcgtac
     2701 gccgctggct ccgacgccaa gctctgttgt gtccgttacg gcaacgtcgt cggctctcgt
     2761 ggatcggtcg tgccggtctt ccgcgagcag atcgcgtcgg gccagctcac catcaccgat
     2821 gcccgcatga cccggttctg gatcaccctc gacgaagcgg tcgacttggt gctctacgcg
     2881 ctcgagcaca ccgagggcgg ggaggtgttc gtgccgaaga tcccgtcgat gaaggtcgtc
     2941 gatctcgccg acgcgatggc gcagggcatc ccgaccgtcg aggtcggtat ccgtcccggc
     3001 gagaagctcc acgaggcgct cctcaccgag gacgaggccc ggcactccgt ggacgccggt
     3061 gacgtctacg ttgtgctgcc cgaacacccg tggtggggca tgggtggtgc atgggaggac
     3121 gcgacaccgc tgcccgacgg gttcgcctac acgagcgaca acaacgacga ctggctgacc
     3181 gcggaccagc tgacggccct cttggcatga ttccctacgg ccgccagacg atcgaggagg
     3241 acgacatcgc cgcggtcgtc gccgtgcttc ggagtgacct cctgacgcaa gggccggccg
     3301 tcgaggagtt cgaggcgcgc ctctgcgaga tcaccggggc ccagtacgcg gtcgcggtgt
     3361 cgagtggcac cgcggcgctg catgctgccc tccatgccgg cggcatcact gcgagccaca
     3421 ccgtcgccac ctcaccgctg tcgttcgcag cgagcgccaa cgcggcccgc tatctgggtg
     3481 ccggtgtgag cctgatcgac atcgaggcgg acacgctcaa cctcgatccc accgccgtcc
     3541 ccgagcacgc cgaggcgttg gttgcggtgc actacgccgg cctccccgtg gacctcggcc
     3601 ggctgaaccg ccggccccgg gtgatcgtcg aggatgccgc ccacgcgatc ggcgccgcca
     3661 cacccgacgg tccggtcggc aactgcgccc gcagcgacat gtgttgcttc tcgttccatc
     3721 ccgtgaagac ggtgaccacg ggcgagggcg gtgcggtcac gaccaactcg cccgacctag
     3781 cggatcgctt gcgccgcttc cgccatcacg gcatctcgaa gaacgcaggg gatccgttct
     3841 ggaagtacga catcggcgac ctcggctaca actaccggct gaccgacctg caggccgcgc
     3901 tcggtacgag ccagctcggc aagctcgaac ggttcatcga tgcccgtcag gcgatcgcag
     3961 cgcgctaccg ggtggcgttc gccggcgacg agcgcatcga cctgccgccg gatgctcccg
     4021 ccgggttccg gcacgcctac cacctgttcc cgattcgggt ggacgatcgt gaccgggtgt
     4081 tcgacgagct gcggagccgg ggcatcggcg tccaggtcca ctacgttccg atgtaccgat
     4141 tcacggattt cggcggagat gccgatggat ccggtgtgac cgactacccc gagaccgagg
     4201 ccgcgttcga gcggctgatc tccctgccga tgttccccgg cctgactccc gaggaccagg
     4261 acatcgtcat cacgaccctg ttggaggtcc tgtgacgtcc ctcgatcgct cccgtgagtg
     4321 gtgggcacgc gccgagcgcg tgatcccgtt ggggacgcag accctgtcga agagccccac
     4381 ccagttcgtc cagggcgcgt cgccgatctt cctcgaacac ggtcggggtg cccacgtgtg
     4441 ggacgtcgac ggcaacgagt tcatcgacta cccaatggcg ctgggcccgg tgatcctcgg
     4501 ctacgccgag ccggtcgtga accgggcgat cgagcgccag ctggccgacg gcatcacgtt
     4561 cacgctgatg catcccctcg aggtcgaggt ggccgagcgc atcgtggcga tgtgccccgg
     4621 cgtcgaggcc gtccgcttcg ccaagaccgg ctcggacgcg gtcagtgccg ccgtgcgagc
     4681 cgctcgcgcg ctgacgagcc gcgatgcggt agccgtcgcc ggccaccacg gttggcacga
     4741 ctggtacgtc ggcacgacga cccgcgacct cggtgttccg ggg
//
LOCUS       DAOSOA010000266         4760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_17955_length_4760_cov_5.021254, whole genome shotgun sequence.
ACCESSION   DAOSOA010000266 DAOSOA010000000
VERSION     DAOSOA010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17955_length_4760_cov_5.021254"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKY13_13675"
     CDS             <1..314
                     /locus_tag="PKY13_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT81037.1"
                     /translation="GERQLVALARAQLADPGLLILDEATSAVDPETERALSDALLRLA
                     AGRTTISVAHRLSTAEAADLVVVFDRGQIVEVGSHDELVAEGGTYAGLYASWVGNTRA
                     A"
     gene            425..1417
                     /locus_tag="PKY13_13680"
     CDS             425..1417
                     /locus_tag="PKY13_13680"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOT81038.1"
                     /translation="MTTKAPLRVAVTGAAGQIGYSLLFRIASGAMLGDDQPVILQLLE
                     ITPALGALSGVAMELDDCAFPLLSGIVQTDDPNVAFGDADVALLVGAMPRKDGMERSD
                     LLSANGGIFKPQGQALSASAKKDVKVLVVGNPANTNSLIAMNNAPDLDPGRFTAMTRL
                     DHNRAIAQLAAKTGTTVSDITKMTIWGNHSATQFADLFHAEVKGQNAAELVNDEAWYA
                     DTYIPTVAQRGAAIIKARGLSSAASAANAAIDHIRSWHLGTPEGDWVSMAIPSDGSYG
                     VPEGLISSFPCVCKDGKYEIVQGLDINEFSRARIDKSVRELAEERDAVKELGLI"
     gene            complement(1487..2326)
                     /locus_tag="PKY13_13685"
     CDS             complement(1487..2326)
                     /locus_tag="PKY13_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOT81039.1"
                     /translation="MPELPEVRVHAERLSADYRGSKLTGFVPLSFTALKTFSPAPDVA
                     VGHELVGVRTRGKYLLMDFGPAVFVIHLMQGGRLTPDPKLSKKPRGGLCRWKFDGRDA
                     LLFTEAGTEKKAGVWVIAPPVDDVEPLAHLGPEADQLSPDELGAVLDEAKGARLHGVL
                     RDQRRLAGLGRRLANEICWTAQLSPFAACNKIDAEGRVRLGAAIVSCVGESTDDERTR
                     DTMVKSAARLAHVHHRKGEPCDRCGDVIREVSYNAYQVDYCATCQTAGKILADNTTSK
                     FLK"
     gene            complement(2331..2828)
                     /locus_tag="PKY13_13690"
     CDS             complement(2331..2828)
                     /locus_tag="PKY13_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81040.1"
                     /translation="MRARHLAPLLIAVLALSACGGGGGKITIGEAASGAPATTAGNDD
                     SAATIDEATDDALTDDTMADDTTPAESSDGGVEPNMDKCIEVSEAFSSIAGSASDMTP
                     DGAQAALDKMKEVLPSDLRDDIQTMVDVYASSPSDIGEAVSSQDFIDANNAVTDYLAQ
                     ICGAG"
     gene            complement(2858..3259)
                     /locus_tag="PKY13_13695"
     CDS             complement(2858..3259)
                     /locus_tag="PKY13_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81041.1"
                     /translation="MSIPYLSAAWIDAADALLRTVVVDPPVVGPAFTVETVVTDAPDG
                     VVGYVLEFDGSAVRARPAGSGERSTVRLTQSYTLAAGVAAGEVGAQAAFLAADIQVGG
                     DVATLIANAGLIAQVGDALRPLRSDTTFVPA"
     gene            3299..4225
                     /locus_tag="PKY13_13700"
     CDS             3299..4225
                     /locus_tag="PKY13_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81042.1"
                     /translation="MPTPNPADERRHTPDDSLLWNESYYLDWFSEDGSLGGYIRIGFN
                     PGLGTVWYWACLVGPGRPLVTVVSNEVPLPSSAQSLEVRTEGIWADHTVEVPLDHMTC
                     NLEAFGLQVDDPADMYHDQRGTLVPFGFELDWETDRAAYLWPPVTPRYEIPCRVHGRV
                     LLGDEVIEVDGWGQRDHSWGAARDWWSLGWCWSAGRLDDGTRWHSAGAFSPDHNWGVS
                     YRLDAGATEFSECFEIDVTSELGREGFADSARLVMGDLALNVEPLAWSPVLLVHPDGR
                     EARFPRALARFTADDGRSGLGWIEFNQPPAPS"
     gene            complement(4242..>4760)
                     /locus_tag="PKY13_13705"
     CDS             complement(4242..>4760)
                     /locus_tag="PKY13_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81043.1"
                     /translation="PVMRTRLTALAVVTLCFTLVGCSSGDGKITIGKAAEGASTTVVT
                     DETTEDTTADSTESPGDSSEMSIPDLGTIPEGLPEDMAKCAELATAMSTMAMSAMGSP
                     IPEETKAQIAEIRAGLPDDIAKDLDTLVDAYSKLSEAGGDLGKAAEAVADPKFTAASD
                     RLSAYFQESCAG"
BASE COUNT          712 a         1662 c         1613 g          773 t
ORIGIN      
        1 tcggcgagcg ccagctggtg gccctggccc gggcgcagct cgccgatccc ggccttctga
       61 tcctcgacga ggcgaccagt gcggtcgacc cggagaccga gcgggcgttg tcggacgcgc
      121 tgctccgcct cgctgccggt cggacgacga tcagtgttgc ccaccgcctc tccaccgccg
      181 aggccgccga cctcgtagtc gtgttcgacc gaggccagat cgtcgaagtg ggctcccatg
      241 acgagctcgt ggcggagggt ggtacctacg ccgggttgta cgcatcatgg gtcggcaaca
      301 cccgcgccgc ctgaccgggc ccgggccccg tcgccccgag atgagggccc tggagcaccc
      361 gctgtagtct cggagtaccc gtggcggcac cgaggtcgcc cagcgtcaga tcgaggagat
      421 ctctgtgacc accaaggccc ccctgcgtgt cgccgtgacc ggcgccgccg gccagatcgg
      481 ctattccctc ctgttccgca tcgccagtgg cgccatgctg ggcgacgacc agccggtcat
      541 cctgcagctg ctcgagatca ccccggcgct cggcgccctc agcggcgttg ccatggagct
      601 cgacgactgc gcgttcccgc tcctctcggg catcgtccag accgacgatc ccaacgttgc
      661 gttcggtgac gccgacgtgg cgctcctcgt gggtgccatg ccccgcaagg acggcatgga
      721 gcgctccgac ctcctgtcgg ccaacggcgg catcttcaag ccccagggcc aggccctctc
      781 ggcctcggcg aagaaggacg tgaaggtcct cgtcgtcggc aacccggcca acaccaactc
      841 gctcatcgcg atgaacaacg ctcccgacct cgacccgggc cgcttcaccg ccatgacccg
      901 cctcgaccac aaccgtgcca tcgcccagct cgcggcgaag accggcacga ccgtgtccga
      961 catcaccaag atgacgatct ggggcaacca ctccgccacg cagttcgccg acctcttcca
     1021 cgccgaggtc aagggccaga acgccgccga gctggtcaac gacgaggcct ggtacgccga
     1081 cacgtacatc cccaccgttg cccagcgcgg cgccgccatc atcaaggccc gcggcctgtc
     1141 gtcggcagct tcggccgcca acgccgccat cgaccacatc cgttcgtggc atctcggcac
     1201 tcccgagggc gactgggtct cgatggccat cccgtccgac ggctcctacg gcgtgcccga
     1261 ggggctcatc agctccttcc cgtgcgtctg caaggacggc aagtacgaga tcgtgcaggg
     1321 cctcgacatc aacgagttca gccgtgcccg catcgacaag tccgtccgcg aactcgccga
     1381 agagcgcgac gccgtgaagg aactcggcct catctgagcc cgacgttggg cttttgtgtt
     1441 gcgaggaacg acgcacgcgt cgttcctcgc aacacaaaac ccccaactac ttcaggaact
     1501 tcgacgtggt gttgtccgcg aggatcttgc cggccgtctg gcacgtggcg cagtagtcga
     1561 cctggtaggc gttgtagctg acctcgcgga tgacgtcgcc gcagcgatcg cacggctcgc
     1621 ccttgcggtg atgcacatgg gcgagccggg ccgcggactt caccatcgtg tcccgggtgc
     1681 gctcgtcgtc ggtcgactcc ccgacacacg acacgatcgc cgcaccgaga cggactcggc
     1741 cctcggcgtc gatcttgttg caggcggcga acggcgacag ctgggcagtc cagcagatct
     1801 cgttggcgag ccggcggccg agcccggcga ggcgtcgctg atcgcggagg acgccatgga
     1861 ggcgtgcccc cttggcctcg tcgaggaccg ctccaagctc gtcgggcgac agctggtccg
     1921 cctccgggcc gaggtgggcg aggggttcga cgtcatcgac ggggggagcg atcacccaca
     1981 cgccggcctt cttctcggtg cccgcctcgg tgaacagcag tgcgtcgcgt ccgtcgaact
     2041 tccagcggca cagcccgccg cggggcttct tcgagagctt gggatcaggg gtcagacgtc
     2101 cgccctgcat caggtggatg acgaacaccg caggaccgaa gtccatcagc aggtacttgc
     2161 cgcgcgtcct gactccgacg agttcgtgtc cgacggcgac gtcgggcgca ggactgaacg
     2221 tcttgagggc cgtgaacgag agcggcacga agccggtcag cttgctccct cggtagtcgg
     2281 cgctgaggcg ctcggcatgg acgcgaacct cggggagctc gggcatcggc tcagccggcg
     2341 ccgcagatct gggcgaggta gtcggtcacg gcgttgttgg catcgatgaa gtcctggctc
     2401 gacaccgcct cgccgatgtc ggaggggctc gacgcgtaca cgtcgaccat cgtctggatg
     2461 tcgtcgcgga ggtcgctcgg cagcacttcc ttcatcttgt cgagcgccgc ctgtgcgccg
     2521 tcgggggtca tgtccgacgc cgatcccgcg atgctggaga atgcttcgct gacctcgatg
     2581 cacttgtcca tgttcggttc gacgccacca tcgctggact cggccggcgt cgtgtcgtcg
     2641 gccatcgtgt cgtcggtcaa cgcgtcgtcg gtggcctcgt cgatcgtggc agcgctgtcg
     2701 tcgtttccgg cagtcgtggc cggggcaccc gacgcagcct caccgatggt gatcttgcca
     2761 ccgccccctc cacaggcgct cagggcgagg acggcgatca ggagcggggc gaggtgacgg
     2821 gcgcgcatga tctcaggcta tcgacccgga cggcctgtca ggcgggaacg aacgtggtgt
     2881 cggaacgcaa cgggcgcaac gcgtcgccga cctgggcgat gagcccggcg ttggcgatga
     2941 gtgtcgccac gtcgccgccg acctggatgt ccgcggcgag gaacgccgcc tgggcgccga
     3001 cctccccggc ggcgacaccg gccgcgagcg tgtagctctg cgtgagccgg acggtggacc
     3061 gctcgccgga gcccgccggc cgggcccgca cggcggaacc gtcgaactcg agcacgtagc
     3121 ccaccactcc gtcgggtgcg tcggtgacca ccgtctcgac ggtgaacgcc ggaccgacga
     3181 cgggcggatc gaccacgacg gtgcggagca gggcgtcggc cgcatcgatc caggcggcgc
     3241 tgagataggg gatcgacatc gggcgcacac taccggcgcc cgccctacga tccgatccat
     3301 gcccacgccg aaccctgccg acgagcgtcg ccacaccccc gacgattcgc tcctgtggaa
     3361 cgagtcctac tacctggact ggttctccga ggacggttca ctcggcggct acatccgcat
     3421 cggcttcaat ccgggtctcg ggaccgtctg gtactgggcg tgtctcgttg gtccggggcg
     3481 accgctcgtg accgtggtga gcaacgaggt gcccctgcca tcgtcggcgc aatcgctcga
     3541 ggtccgcacc gaggggatct gggcggacca caccgtcgag gttcccctcg atcacatgac
     3601 ctgcaatctc gaggcgttcg gcctgcaggt cgacgatccg gccgacatgt accacgacca
     3661 gcgaggaaca ctcgtcccct tcggcttcga actcgactgg gagaccgacc gcgccgccta
     3721 tctctggccg ccggtgacgc cccgctacga gatcccgtgt cgagtccacg gtcgggtgct
     3781 gctcggcgac gaggtgatcg aggtcgacgg atggggccaa cgcgaccact cgtggggagc
     3841 ggcacgcgac tggtggtcgc tcggatggtg ctggtcggcc ggtcgactcg acgacggaac
     3901 gcggtggcac agcgccggag cattctcgcc ggaccacaac tggggtgtgt cctaccggct
     3961 cgacgccggc gccacggagt tctccgagtg cttcgagatc gacgtcacgt cggagctggg
     4021 gcgggagggc ttcgccgaca gcgctcgcct cgtgatgggc gacctcgccc tgaacgtcga
     4081 gccgctggcg tggtcgccgg tgctgctcgt tcaccccgac ggtcgcgagg cgcgctttcc
     4141 gagggcgctg gctcggttca ccgccgacga cgggcggagc gggctgggtt ggatcgaatt
     4201 taaccagcct ccggcgccgt cctgatgggc gagccggtgg gtcagccggc gcagctctcc
     4261 tggaagtagg ccgacagccg gtcgctcgcc gccgtgaact tggggtcggc aacggcttcg
     4321 gccgccttgc cgagatcgcc gcccgcttcg ctgagcttgg agtaggcgtc gaccagtgtg
     4381 tccagatcct tcgcgatgtc gtcgggcagc ccggcgcgga tctccgcgat ctgcgccttc
     4441 gtctcctcgg ggatggggga gcccatcgcc gacatcgcca tcgtcgacat ggccgtggcc
     4501 aactcggcgc acttcgccat gtcctcggga aggccctcgg gaatggtgcc gaggtcgggg
     4561 atgctcatct cgctggagtc cccgggtgac tcggtggagt ccgccgtcgt gtcctcggtg
     4621 gtctcgtcgg tgacaaccgt ggtcgacgca ccctcagccg ccttgccgat ggtgatcttc
     4681 ccgtcgccgc tgctgcaccc cacgagggtg aagcagagcg tgacgacggc gagggcggtg
     4741 aggcgggtgc gcatgaccgg
//
LOCUS       DAOSOA010000267         4744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_18030_length_4744_cov_6.238430, whole genome shotgun sequence.
ACCESSION   DAOSOA010000267 DAOSOA010000000
VERSION     DAOSOA010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18030_length_4744_cov_6.238430"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PKY13_13710"
     CDS             complement(<1..828)
                     /locus_tag="PKY13_13710"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOT81044.1"
                     /translation="MTSVLDPTSRSALPTRADVRAAIPDDCFERRTGRSLAYLASSLA
                     LVVGMAVAAVAWIPMTWAWLPAWIVYAIANGTAATGLWVIAHECGHGAFSANARLQDA
                     VGFTLHSALLVPYFSWQRSHSVHHARTNHLDEGETHVPKRADHPRAVAEQRFRATVGE
                     RAGAALAITKTLLLGWPAYLVAGVSGSDRRGRSNHFWPIRPFAGELFPPRWHRRVWVS
                     TAGVAAVVALLVAAAVAFGPLAVVAAYVGPYVVVNAWLVGYTWLQHTDVDVPHYADDE
                     "
     gene            856..1761
                     /locus_tag="PKY13_13715"
     CDS             856..1761
                     /locus_tag="PKY13_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT81045.1"
                     /translation="MSEHPSAPEWFRHALAVPYEDHTVSVAGCDIHYLAWGEPGRQGL
                     VFVHGGGAHAHWWTHIAARFAGEFRVVAIDLSGHGDSGFRDVYALEQWTEEVMAVVAD
                     SGITGMPVIVGHSMGGFVTVATAALNGDAVAGIIVCDSPITEPDPEVQSYRLKQAFGR
                     PRTYEDFEEAVARFRTVPGQDHSLDYVMDHVARHSLRRVEGGWRWKFDENIFVQFATT
                     MRGIALPYLHQVDCRVALLRSEYGLITPDIGQEMFEALGRVAPVIELPEAGHHAMLDV
                     PLTLLTALRTLLADWEHSYREVRSW"
     gene            complement(1795..2691)
                     /locus_tag="PKY13_13720"
     CDS             complement(1795..2691)
                     /locus_tag="PKY13_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT81046.1"
                     /translation="MKIRFGVGLGVGQSLDTPAQLGDVVDLLEGLGFDSLWMSDRVTG
                     GALDPIAALAFAAGRTTKLKLGTNVLILPGRDPFLMARQLAAVDQLSGGRLLPAVGLG
                     SPSKADRPPFGVPKGTRAQRFEDGLAIIRELWRDGGAVPHPDGGKPMTIDPRPTKPLE
                     IWFGGRSVPALQRAGRLSDGWIGSFQTPEQCGEARRVIVDAAADAGRSIDDDHYGTAI
                     FYARERRSEVGEFVVKALTESGGGHYVPEVLLPLGADELAAVIADYVAVGLSKFVLVP
                     DQPDDWSAELTWLREVTGPLET"
     gene            complement(2742..3710)
                     /locus_tag="PKY13_13725"
     CDS             complement(2742..3710)
                     /locus_tag="PKY13_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT81047.1"
                     /translation="MKRSSRHLVATVMVDEVSGLEPAIAAELFAADWGADFDVTWYEH
                     RFCTERPGRLRVSGGMDVFVDDDLSVLRSADTIIIPGWGRRHPPTAAVTGELRAAHDR
                     GARIVSFCTGAFALAAAGLLDGRRATTHWGHAEEFAQRFPEVEFDPAVLYVHDDRILT
                     AAGSASCIDLGLYIIRQDYGADIANLVARELVVPPHRDGGQAQYITTPVAVCPDEDPL
                     SETLAWALEHLDQPLTLDALAARSMMSSRTFSRRFAAATGTTPHKWVVHQRVSAAQRL
                     LETTTLSIEVIAERCGFGSAASLRAQFQQTVRSSPQAYRRTFARPA"
     gene            3981..>4744
                     /locus_tag="PKY13_13730"
     CDS             3981..>4744
                     /locus_tag="PKY13_13730"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT81048.1"
                     /translation="MATTTTATKATEGTLSDVSDVPVQYRVIHGHRRAFRMLGSGPAL
                     LLLHGIGDSSQTWVPLMRRLARRYTVLAPDLLGHGASDKPRADYSVAAYANGMRDLME
                     VLGIDRATVVGHSLGGGVAAQIAYQYPERCERLVLVASGGVARDVNPMLRIAAAPFAE
                     LAIPALQWPLGRLGGSLILSALRALGTDIGRDATELRAVFEGMPDGSSRGAFTRTLRS
                     VVDWRGQVVTMLDRCYLAEAVPILLVWGTHDAVIPV"
BASE COUNT          705 a         1599 c         1733 g          707 t
ORIGIN      
        1 ctcgtcgtcg gcgtagtggg gcacgtcgac gtcggtgtgc tgcaaccacg tgtagcccac
       61 gagccaggcg ttcaccacca catacggtcc gacgtaggcg gcgacgacgg cgaggggccc
      121 gaatgccacg gccgccgcaa cgaggagggc aacgacggcg gcgactccgg cggtcgacac
      181 ccacacccgg cggtgccacc gcggcgggaa gagctcgccg gcgaacgggc ggatcggcca
      241 gaagtggttg ctgcggccac gccggtcgga gcccgacacc ccggcgacga gatacgccgg
      301 ccagccgagc aggagcgtct tggtgatcgc gagcgcggca ccggcccgct cgccgaccgt
      361 ggcgcggaac cgttgctcgg ccacggctcg ggggtggtcg gcgcgtttgg gaacgtgcgt
      421 ctcgccctcg tcgagatggt tggtgcgggc gtggtgcacc gagtggctgc gttgccagga
      481 gaagtagggc acgaggagcg ccgagtgcag cgtgaacccg accgcgtcct gcagtcgggc
      541 gttggccgag aacgcaccgt ggccgcactc gtgggcgatg acccacaggc ccgtcgctgc
      601 agtgccgttg gcgatggcgt acacgatcca cgccggcagc cacgcccagg tcatcgggat
      661 ccaggcgacg gcagcgaccg ccatgccgac gacgagcgcc agcgacgatg cgaggtaggc
      721 gagggagcga ccggtgcggc gctcgaagca gtcgtcgggg atcgcggcgc gcacgtccgc
      781 ccgtgtcgga agggcagaac gagaggtggg gtccagtacc gaggtcatcg ggcggcgagc
      841 gtaggctgcg cggccatgag tgagcatcct tccgcccctg agtggttccg acacgccctc
      901 gccgtgccgt acgaggatca caccgtgtcg gtcgccgggt gcgacatcca ctacctggca
      961 tggggcgagc ccgggcggca gggcctcgtg ttcgtgcacg gcggtggtgc gcacgcccac
     1021 tggtggaccc atatcgcagc gcggttcgcc ggcgagttcc gggtggtggc gatcgatctg
     1081 tcgggtcatg gcgacagcgg gttccgtgac gtctacgcac tcgagcagtg gaccgaggag
     1141 gtgatggcgg tggtggccga cagcggcatc accggcatgc ccgtcatcgt cggccacagc
     1201 atgggcggat tcgtgacggt cgcgacggcg gcgctcaacg gcgacgccgt cgccggcatc
     1261 atcgtgtgcg attcgccgat caccgaaccc gatcccgagg tccagtccta ccgcctgaag
     1321 caggcgttcg gccggccgcg gacctacgag gacttcgaag aggccgtcgc ccgcttccgc
     1381 accgtgccgg ggcaggacca ctcgctcgac tacgtgatgg accacgtggc caggcactcg
     1441 ctgcgccgtg tcgagggcgg ctggcgttgg aagttcgacg agaacatctt cgtgcagttc
     1501 gcgacgacca tgcgcggtat cgccctcccg tacctgcatc aggtcgactg tcgggtcgcg
     1561 ctcctccggt cagagtacgg cctcatcacc cccgacatcg gccaggagat gttcgaggcg
     1621 ctgggccggg tggctccggt catcgagctg cccgaggccg gccaccacgc catgctcgac
     1681 gtgccgctca cgctcctcac ggcgttacgc acgttgctcg ccgactggga gcactcctac
     1741 cgcgaggtcc ggtcgtggtg aacccggccc cgccggagcg gagttgggcc ccggtcaggt
     1801 ctcgagcggt ccggtcacct cgcgcagcca cgtgagttcg gcgctccagt cgtcgggctg
     1861 gtcgggcacc agcacgaact tcgagagtcc gacggcgacg tagtcggcga tgaccgccgc
     1921 gagctcgtcg gcgccgagtg ggagcaggac ctcgggcacg tagtggccgc cgcccgactc
     1981 ggtgagtgcc ttgacgacga actcgccgac ctcgctgcgg cgttcccggg cgtagaagat
     2041 cgcggtgccg tagtggtcgt cgtcgatcga gcgcccggcg tcggccgcag cgtcgacgat
     2101 cacccgccgg gcctcgccgc actgttcggg cgtctggaag ctgccgatcc acccgtcgct
     2161 caggcgcccg gcccgctgga gcgccgggac gctgcggcca ccgaaccaga tctcgagcgg
     2221 tttggtcggt cgtggatcga tcgtcatcgg tttgccgccg tcggggtggg gcaccgcgcc
     2281 gccgtcacgc cagagctcgc ggatgatcgc gaggccgtcc tcgaaccgct gcgcccgggt
     2341 gcccttgggg acgccgaacg gcgggcggtc ggccttcgac ggcgaaccga gtccgaccgc
     2401 cgggagcagt cgcccgccgc tcagttggtc gaccgccgcg agctggcggg ccatgaggaa
     2461 cgggtcgcgg ccggggagga tgagcacgtt cgtcccgagc ttcagcttcg tcgtgcgccc
     2521 cgccgcgaac gcgagggcgg cgatggggtc aagcgcgccg ccggtcaccc ggtccgacat
     2581 ccacaacgag tcgaacccga gtccttcgag gaggtcgacg acgtcgccga gctgcgccgg
     2641 cgtgtcgagg gactgaccga cgccgagacc gacaccgaat cggatcttca tgtgcacccc
     2701 tttgtgtggg ccggttcggg cgaatcggct gccgtcccgc gtcaggctgg gcgggcgaac
     2761 gtccgacgat acgcctgcgg cgaggagcgc accgtttgtt ggaactgggc tcgcaacgac
     2821 gccgcggatc cgaacccgca gcgttcggcg atcacctcga tcgacagcgt cgtcgtctcg
     2881 aggagtcgct gggcggcgga cacgcgctgg tggacgaccc acttgtgggg agtggtgccg
     2941 gttgctgcgg cgaagcgccg ggagaacgtt cggctgctca tcatcgaccg ggctgccagg
     3001 gcatcgaggg tgaggggttg gtcgaggtgc tcgagcgccc aggcgagggt ctcgctgagc
     3061 gggtcctcgt cggggcacac ggcgacgggc gtggtgatgt actgggcctg gccgccgtcg
     3121 cggtgtggtg gcacgacgag ctcacgggcc acgaggttgg cgatgtcggc cccgtagtcc
     3181 tgccggatga tgtagagccc caggtcgatg cacgacgccg agccggcggc ggtgaggatg
     3241 cggtcgtcgt gcacgtagag gacggccggg tcgaactcga cctcggggaa gcgctgtgcg
     3301 aactcctcgg cgtggcccca gtgcgtcgtg gcgcggcggc cgtcgaggag cccggcggca
     3361 gcgagggcaa aggcgccggt gcagaacgag acgatgcgag caccgcggtc gtgggccgca
     3421 cggagttccc cggtcacggc cgcggtcgga gggtgtcggc gcccccaccc cgggatgatg
     3481 atcgtgtcgg cgctgcggag gacggacagg tcgtcgtcga cgaacacgtc catgcctccg
     3541 ctgacgcgca ggcgacccgg ccgctcggtg cagaacctgt gctcgtacca cgtcacgtcg
     3601 aagtcggcgc cccagtcggc agcgaacaac tccgcggcga tggccggttc gaggccggag
     3661 acctcatcga ccatcaccgt ggcgaccagg tggcgcgacg atcgtttcat ggctggatct
     3721 tcgcgcccgt ggtgattcga gacaatggcg agtttctcgc gaacagtgtc aagtcagcca
     3781 ctcgtcggta tcgggaggcg ggcggacaat gagcccatgg atcagaactg gctctcggca
     3841 cagaacaact ccgccctcta ccaggcccgg atgcgtgagc tcagccgacc tcggctacgt
     3901 cgactcggcg agctgacccc gaaacgcgat gttcacggtc ggactcgtcg ctggaaccac
     3961 cgagccgaca gacttggggg atggcgacga cgaccaccgc gaccaaggcg accgagggaa
     4021 cgctgagcga cgtcagtgac gtgccggtcc agtaccgggt catccacggg caccggcgtg
     4081 cgttccgcat gctcggcagc ggccccgctc tgctcctcct gcacggcatc ggcgacagca
     4141 gccagacgtg ggtcccgctc atgcgccgtc ttgcccgacg gtacacggtc ctcgcgcccg
     4201 acctcctggg tcacggagcg tccgacaaac cccgcgccga ctactcggtc gcggcgtacg
     4261 ccaacggaat gcgcgacctc atggaggtcc tgggcatcga tcgcgcgacc gtcgtcggcc
     4321 actcgctcgg cggcggtgtc gctgcgcaga tcgcctacca gtaccccgag cgatgtgagc
     4381 ggctggtcct cgtggcgtcc ggtggtgtgg cccgcgacgt caaccccatg cttcgcatcg
     4441 ccgcggcacc gttcgccgag ctcgcgatcc cggcgttgca gtggccgctc gggcggctcg
     4501 gcggttcgct gatcctgtcg gcgctgcgtg cgctcggcac cgacatcggc cgcgatgcga
     4561 ccgagctgcg agcggtattc gaggggatgc ccgacggcag ctcgcggggt gccttcaccc
     4621 gcaccctccg atccgttgtc gactggcggg gccaggtcgt gacaatgctc gaccggtgct
     4681 acctcgccga ggccgtcccg atcctgctcg tgtggggcac tcacgacgcc gtgatccccg
     4741 tcga
//
LOCUS       DAOSOA010000268         4739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_18056_length_4739_cov_7.035440, whole genome shotgun sequence.
ACCESSION   DAOSOA010000268 DAOSOA010000000
VERSION     DAOSOA010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18056_length_4739_cov_7.035440"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKY13_13735"
     CDS             <1..247
                     /locus_tag="PKY13_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOT81049.1"
                     /translation="NAVLADKATLTKILTLHVVAGKMSAADLAKAGTATTVEGQDITF
                     SGDASSLQVNGQAAVVCGDVQVANATVHIIDSVLMPS"
     gene            434..1015
                     /gene="sigK"
                     /locus_tag="PKY13_13740"
     CDS             434..1015
                     /gene="sigK"
                     /locus_tag="PKY13_13740"
                     /inference="COORDINATES: protein motif:HMM:NF007228.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF RNA polymerase sigma factor SigK"
                     /protein_id="HOT81050.1"
                     /translation="MIDLLTRRGRSLPKSADELLVQVAKGDQEAYAEFYEIIVERVFG
                     IVRSILRDPARSEEVTQEVMLELWRTAPRYERRLGAATSWALTIAHRRAVDRVRSEQA
                     SRNRDERHALEHAGATAPDTGAVVVDLLDRERVANALGELTEPQRESIELAYFGGHSH
                     SEVAALLDLPLGTVKTRIRDGLVRLRDRLEVTS"
     gene            1012..1755
                     /locus_tag="PKY13_13745"
     CDS             1012..1755
                     /locus_tag="PKY13_13745"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOT81051.1"
                     /translation="MSNASADEHDSLNLAGMAGLYALDALEGEELLKFEALLATNAEL
                     RDEVAGFRATAGRLSEVSAAMPPAALRTNVLAQVAMTRQDPPVIRLDERRRASAGRRA
                     LLGAVAAGLIVLAGFGGYAIADNGDATSSELASILARSDAEVVALKGVGDDAAAGRVV
                     VSPEAGKVIVISTSIPPVEPGRTYELWRVDADGSVHKAGLFEPDGSGKVEAAIEVSLD
                     GAIGFAVTDEPDGGSEQATTPILMQAELA"
     gene            1820..3073
                     /locus_tag="PKY13_13750"
     CDS             1820..3073
                     /locus_tag="PKY13_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT81052.1"
                     /translation="MSHLAPVWYKVTDLDVVSGEGCWVTTGAGDRYLDFTAGIAVTST
                     GHCHPTVVAAIQAQAARFIHAQANCYTHPLLGELADALDRITPAGIDTFFFSNSGAEA
                     TEAAVKLAKQATGRPNVIVFEGSFHGRTHLTMAMTTSKTVYRSGHSPLPSGVFVAPFP
                     RSLGAGTLDDRIDAALEAVRHLLLSQTSPAETAAIIIEPVLGEGGYEPAPARFLHGLK
                     AICAEHGMLFVADEVQAGFGRTGTMFAVETTGIEPDILIMAKGIASGFPLSAIGSSAE
                     IMAAWPTGSHGGTYGGNPIGCAAALATIEVLTSDGFLANVNDRGEQLRSGLSKLAGDH
                     PVIADVRGPGLMVAAEFRDPATGAPATARTAAIIEHCRTESKLLLMNAGTYGNVIRFM
                     APLVVTADEVDLALAAVAAGLEASA"
     gene            3070..3789
                     /locus_tag="PKY13_13755"
     CDS             3070..3789
                     /locus_tag="PKY13_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT81053.1"
                     /translation="MTDDRLYFRQLLAGRDFATTDPIARQMVNFAYLIGDRVTGECVV
                     VDPAYDVAGLAAIAAADGMTISGALVTHYHPDHCGGEMMGFHIEGVEALLETHDVPIH
                     VQADELPWVMRVTNCTEPDLVGHAAGDIVTIGEIPIELIHTPGHTPGSQCFLVDNRLV
                     SGDTLFLDGCGRTDLPGGNPEDLYLSLTQRLSKVPDDAVLYPGHQYSPAPSASLGETR
                     QHNVVFRPKTAEQWMMMFGGA"
     gene            3789..>4739
                     /locus_tag="PKY13_13760"
     CDS             3789..>4739
                     /locus_tag="PKY13_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT81054.1"
                     /translation="MAVLGPDPDQLPPGWNETRPPRWLPRAVLAAAIGVAGFMVAQYL
                     FVKLSDLLTILLVSLFLSFAVEPAVDYLAERGWRRGSATGLVFVVLLVLSTVLVWVVV
                     DLVIQQVRTLVNDSPDLVKDATDWVNKRFDANITTDKIVEQLRKYQGDLASTAGDLGG
                     RVVSVTGSVLGVVFNIFTIGLFSFYLVADGPRFRRSICSVLPPERQRLVLGLWELAIH
                     KTGGYLYSRLLLATFSAASSWIAFAIIGVPSPLALALWMGVVSQFVPVIGTYIGGALP
                     LLIALLNDPPHAIWVIVFLVIYQQLENYLLAPRISAATMAI"
BASE COUNT          737 a         1748 c         1488 g          766 t
ORIGIN      
        1 gaacgccgtc ctcgccgaca aggcgaccct caccaagatc ctcaccctgc acgtggtcgc
       61 cggcaagatg tccgccgccg acctcgccaa ggccgggacg gccaccacgg tcgagggcca
      121 ggacatcacc ttctccggtg acgcctcctc gcttcaggtg aacggtcagg ccgcggtcgt
      181 ctgcggcgac gtgcaggtgg cgaacgccac ggtgcacatc atcgattcgg tcctgatgcc
      241 cagctgatca gccgactgat cgcctcccct tcctggacac ggggaggtgg atgacgggcc
      301 gggtcccgaa ggacccggcc cgtttcgcgc gcctgcgaac ggtctggcga aaaagtgtgg
      361 caatccccca atccgatccg ggatccgccc cgaacggctt tgtatggagg ctcgcatcaa
      421 caaggatcat gaggtgatcg atctcctgac ccgccgcgga cggtcgcttc cgaagtccgc
      481 ggacgagctt ctcgtgcagg tcgcaaaggg cgatcaggag gcgtacgccg agttctacga
      541 gatcatcgtg gagcgggtgt tcggcatcgt gcgctcgatt cttcgtgacc ccgcccggtc
      601 cgaagaggtc acccaggagg tcatgctcga gctctggcgc acagcgcccc gctacgagcg
      661 acggctcggg gcggcgacca gttgggccct caccatcgcc caccgccgtg cggtcgaccg
      721 ggtccggtcg gagcaggcga gtcgcaaccg cgacgagcgg cacgcgctcg agcacgccgg
      781 cgcgaccgca cccgacaccg gcgccgtcgt tgtcgacctc ctcgatcgcg aacgcgtcgc
      841 caacgccctt ggggagctga ccgaaccgca gcgcgaatcc atcgagctgg cgtacttcgg
      901 gggccacagc cacagtgagg tcgccgcgct cctcgatctg ccgctcggca ccgtgaagac
      961 caggattcga gacggccttg tccgtctccg cgaccgtttg gaggtgacct catgagcaac
     1021 gcatccgccg acgaacacga ctcgctcaac ctcgcgggga tggcgggcct ctacgcactc
     1081 gacgccctcg agggcgagga actcctcaag ttcgaggccc tcctcgccac gaacgccgag
     1141 ctgcgcgacg aggtcgctgg cttccgggcc accgcgggac gcctcagcga ggtatcggca
     1201 gcgatgccgc ccgcagctct ccgcaccaac gtcctcgcgc aagtcgccat gacccggcag
     1261 gatccgccgg tgatccgtct cgacgagcgc cgtcgcgcca gcgccggtcg ccgtgcactc
     1321 ctcggagcgg tcgcggcagg actcatcgtg ctcgccgggt tcggtggcta cgccatcgcc
     1381 gacaacggcg acgcaacgtc gtcggagctc gcgagcatcc tcgcccgcag cgacgccgag
     1441 gtcgtggcct tgaaaggggt cggcgacgac gctgccgccg gacgcgtcgt cgtgtcgccc
     1501 gaggcgggca aggtcattgt gatctcgacg tcgatcccgc cagtcgaacc gggccggacc
     1561 tacgagctct ggcgggtcga cgccgacggc agtgtgcaca aggccggcct gttcgaaccc
     1621 gacggctcag gcaaggtcga ggccgccatc gaggtgagcc tcgatggcgc catcgggttc
     1681 gccgtcaccg acgaacccga tggcggttcc gagcaggcga ccaccccgat cctcatgcag
     1741 gcggagctgg cgtaggccga ctccggcgaa aaccgctggc gtgacgtccc gggcacggga
     1801 ttcgcgcaac actggtggga tgtctcatct tgcccctgtt tggtacaagg tcaccgatct
     1861 cgacgtcgtg tcgggtgagg gttgctgggt cacgaccggc gccggcgacc ggtacctcga
     1921 cttcaccgcc ggcatcgccg tcaccagcac cggtcactgc cacccgacgg tcgtcgccgc
     1981 gatccaggcc caagcggcgc ggttcatcca cgcccaggcc aactgctaca cccacccgct
     2041 cctgggcgag ctggccgacg cgctcgaccg gatcaccccc gccggcatcg acaccttctt
     2101 cttctccaac tccggcgccg aggcgaccga ggcggcggtg aagctggcca agcaggcgac
     2161 cggacggccc aacgtgatcg tgttcgaggg gtcgttccac ggtcgcaccc acctcaccat
     2221 ggcgatgacc acgtcgaaga ccgtctaccg gtctggtcac agcccgctgc cgtcgggagt
     2281 gttcgtcgcg ccgttccccc gttccctcgg agcgggcaca ctcgacgacc gcatcgacgc
     2341 ggcgctggag gccgtacgac acctgttgct cagccagacc tccccggccg agaccgcggc
     2401 gatcatcatc gagccggtgc tcggcgaggg cggctacgaa ccggcgccgg cccgattcct
     2461 ccacggcctc aaggccatct gtgccgaaca cgggatgctc ttcgtcgctg acgaggtaca
     2521 ggccggcttc ggccgcaccg gcacgatgtt cgcggtcgag acgacgggca tcgagccgga
     2581 cattctgatc atggcgaaag gcatcgcctc gggcttcccg ctctcggcga tcggttcgtc
     2641 ggcggagatc atggccgcgt ggccgaccgg ttcccacggc ggcacctacg gcggcaaccc
     2701 catcggctgc gccgccgccc tcgcaaccat cgaagtgctc acgtcggacg gcttcctggc
     2761 caacgtgaac gaccggggtg agcaactgcg ctccggcctg tccaagctcg ctggggacca
     2821 cccggtcatc gccgacgtcc gtggccccgg cctcatggtc gccgcggagt tccgcgaccc
     2881 ggcgacgggt gccccggcga ccgcccgcac ggcagcgatc atcgaacact gccgtaccga
     2941 gtcgaagctg ctgctcatga acgccggcac ctacggcaac gtgatccggt tcatggcccc
     3001 gctcgtcgtc acggccgacg aggtcgacct ggccctggca gcggttgcgg ccggcctcga
     3061 ggcgtcggca tgaccgacga tcgtctgtac ttccgtcagc tgctcgccgg ccgcgacttc
     3121 gcgactaccg atccgatcgc gcggcagatg gtcaacttcg cctacctgat cggcgaccgg
     3181 gtcaccggcg agtgcgtcgt cgtcgacccc gcctacgacg ttgcgggtct cgcggccatc
     3241 gccgccgccg acggcatgac gatctcgggg gcgctcgtca cccactacca ccccgaccat
     3301 tgcggcggcg agatgatggg attccacatc gaaggcgtgg aagccctcct cgaaacccat
     3361 gacgtaccga tccacgtcca ggccgacgaa ctcccctggg tgatgcgggt gacgaactgc
     3421 accgaacccg atctcgtcgg ccacgctgcc ggcgacatcg tcaccatcgg cgagatcccc
     3481 atcgaactca tccatacgcc gggtcacaca cccgggtcgc agtgcttcct cgtcgacaac
     3541 cggctcgtct cgggcgacac gctcttcctc gacggctgcg gtcgcaccga cctgcccggg
     3601 ggcaaccccg aagacctcta cctcagcctc acgcaacgtc tctcgaaggt gcccgacgac
     3661 gcggtgctct accccggtca ccagtactcg ccggcgccgt ccgcgtcgct cggcgaaacc
     3721 cgacaacaca acgtcgtctt ccgcccgaag accgccgagc agtggatgat gatgttcggc
     3781 ggagcctgat ggctgtcctc gggcccgacc ccgatcagct cccgccgggc tggaacgaga
     3841 cccgtccacc gcgatggctg ccccgcgccg tgctcgccgc cgcgatcggc gttgccggct
     3901 tcatggtcgc gcagtacctg ttcgtgaagc tgtcggacct gctcacgatc ctgctggtct
     3961 cgctcttcct ctcgttcgcc gtcgagccgg cggtcgacta tctcgccgaa cggggctggc
     4021 gccgcggctc ggcgacgggc ctcgtgttcg tggtgctgct cgtgctcagc accgtgctgg
     4081 tctgggtcgt cgtcgacctg gtcatccagc aggtccgcac gctcgtcaac gactcgcccg
     4141 acctggtcaa ggacgcgacc gattgggtca acaaacgctt cgacgccaac atcacgaccg
     4201 acaagatcgt cgagcagctc cgcaagtacc agggcgacct cgccagcacc gccggcgacc
     4261 tcggcggtcg cgtggtgtcg gtcaccggct ccgtactcgg cgtggtgttc aacatcttca
     4321 ccatcgggct cttctccttc tacctggtcg ccgacggtcc ccggttccgt cgctccatct
     4381 gctcggtgct gccgcccgaa cgtcagcgcc tggtgctcgg gctgtgggag ctcgccatcc
     4441 acaagacggg cgggtacctc tactcccgtc tgctgctcgc cacgttctcg gcagcatcgt
     4501 cgtggatcgc cttcgcgatc atcggggtgc cgtccccgct cgccctcgca ctctggatgg
     4561 gcgtggtctc gcagttcgtg cccgtcatcg gcacctacat cgggggagca ctcccgctgc
     4621 tgatcgccct gctcaacgat ccgccgcacg ccatctgggt catcgtgttc ctcgtcatct
     4681 accagcagct cgagaactac ctcctggcgc cgcgcatctc ggcagccacc atggcgatc
//
LOCUS       DAOSOA010000269         4731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_18093_length_4731_cov_4.998717, whole genome shotgun sequence.
ACCESSION   DAOSOA010000269 DAOSOA010000000
VERSION     DAOSOA010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18093_length_4731_cov_4.998717"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PKY13_13765"
     CDS             complement(<1..579)
                     /locus_tag="PKY13_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT81055.1"
                     /translation="MTDSQALELVPEPVNPSVLLERMLFEMRKVIVGQDRAIERMLVC
                     LLANGHCLLESVPGLAKTLSVETMARTVGGTFNRIQFTPDLLPADIVGTRIYRTSSER
                     FDVELGPIFANFVLADEINRAPAKVQSALLEVMAERHVTIGGTTHPAPNPFLVLATQN
                     PIENEGVYPLPEAQLDRFLMKIVLGYPTPHEEL"
     gene            753..1964
                     /gene="ftsY"
                     /locus_tag="PKY13_13770"
     CDS             753..1964
                     /gene="ftsY"
                     /locus_tag="PKY13_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT81056.1"
                     /translation="MLAQSSVVLPIIIGVVVLLVGSGAFVVSSRSKRGELAPPTTPKA
                     VTTTDRPTELLPSEVAPAEADVDSDVEGDVEAPPSLSEELDALEGIEAAETAADTAVE
                     KATFRNRLSRARGALSGALGSIRGRSAIDDETWDDLEEALIRADVGVGPTTELLDSVR
                     ATVKQNGITTPDELIDAVKAEMTTRLLAPIDLRESANKPSIWLFVGVNGVGKTTTIGK
                     VGKLLTDDGRKVLMAAGDTFRAAAAEQLGTWAERCGADLVRGAEGSDPSSVIFDGIAR
                     GAATGADVVLADTAGRLHTKSNLMDELRKVRRVADKGEGEVTEVLLVLDATTGQNGLI
                     QARQFTDAVELTGVVLTKLDGSAKGGIVFAIQSELGIPVKLVGLGEQAEDLIPFDAEQ
                     FVDALFATDEP"
     gene            2068..3552
                     /gene="ffh"
                     /locus_tag="PKY13_13775"
     CDS             2068..3552
                     /gene="ffh"
                     /locus_tag="PKY13_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT81057.1"
                     /translation="MFEALSDRFEGIFKRLRNSGSLTPDQVDEVLREIRVALLEADVN
                     VRVVRNLQNRIRERAVGLEVSAALTSAQQVIKIVNEELTATLGGETMRITFASKPPTV
                     VLMAGLQGSGKTTNSAKLARWFKAQGRNPLLVGADLQRPAAVEQLRVLGASIDVPVYS
                     EPTDPVAVAQGAVAEARRLGRDVVIVDTAGRLAIDEEMMAQVRDISAAVDPDYTFLVI
                     DAMLGQDAVTVAEGFHNSLALDGVILTKLDGDARGGAALSVKEVVGRPIAFASTGEKL
                     EDFDLFHPDRLAGRILGMGDMLSLIEKAEEAFDAEQSEVAAQRLMDGTFTFDDFLEQM
                     NAIRKMGPLSNIIGMLPGIPKEVRDVEIGDKEVGKVEAIIRSMTTEERTNPDIIDTSR
                     RQRIARGSGTNPTDVGQLITQFKEMRKMMQQMGGGAMKRVKKKTKKGQKKASKGNPSG
                     RTTAKGTLPIPKKEFRLPGLDPLADQLEKAGIEIPGLDLGDRND"
     gene            3629..3922
                     /gene="rpsP"
                     /locus_tag="PKY13_13780"
     CDS             3629..3922
                     /gene="rpsP"
                     /locus_tag="PKY13_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT81058.1"
                     /translation="MAVRLRLMRMGKKKQPTYRIVAADARSPRDGRFIEIVGTYDPRR
                     EPSAIKVDNEKAIAWLKNGAQPSERVEKLLKISGAWDEFEASKPAKKTAKAAK"
     gene            3919..4173
                     /locus_tag="PKY13_13785"
     CDS             3919..4173
                     /locus_tag="PKY13_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOT81059.1"
                     /translation="MSDGTTARAEAVLAHVVTAIVDHPDDVKVSTDTDREPTRINIEV
                     GDGDMGRVIGRRGRVANAIRAVGRAAAAADDVDVDVEFVD"
     gene            4166..4660
                     /gene="rimM"
                     /locus_tag="PKY13_13790"
     CDS             4166..4660
                     /gene="rimM"
                     /locus_tag="PKY13_13790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOT81060.1"
                     /translation="MTDNGAPAPGLLEVGRIGKAHGLKGEVSVRLISDRTERLDRGAV
                     LSTARGDLRVAASRPHQDRWIVQFDGISGRTEAESWHGVVLMGEPIDDPSALFVHDVV
                     GCTVVDADGVERGVVTAVQANPASDLLVLDSGALVPMNFVVGDIDGTTIHVDVPDGLF
                     ELYS"
BASE COUNT          801 a         1597 c         1626 g          707 t
ORIGIN      
        1 tagctcttcg tgcggcgtcg ggtaaccgag gacgatcttc atgaggaagc ggtcgagttg
       61 ggcctcgggc agcgggtaca cgccctcgtt ctcgatcggg ttctgggttg ccagcacgag
      121 gaacgggttc ggggccgggt gggtggtgcc gccgatcgtg acgtggcgct ccgccatgac
      181 ttcgagcagc gccgactgca ccttggcggg cgcccggttg atctcgtccg cgagcacgaa
      241 gttggcgaag atcgggccga gttcgacgtc gaaccgctcc gacgacgtgc ggtagatgcg
      301 ggtgccgacg atgtcggcgg gcagcagatc gggcgtgaac tggatgcggt tgaacgtgcc
      361 gccgacggtg cgtgccatgg tctcgaccga cagggtcttc gccaggccgg gaacgctctc
      421 gaggaggcag tgaccgttgg cgaggaggca cacgagcatc cgctcgatcg cccggtcctg
      481 cccgacgatg accttccgca tctcgaacaa catgcgctcg agcagcaccg acgggttcac
      541 gggctcggga acgagctcga gtgcttggga atcggtcatc gtgggttcct ccgcgtcgga
      601 cgcgtggtcg gggggtcggc tgggctcagc ggtgtaaccg tacgagggat ccgccggaac
      661 gtggtggaaa cgcgctgagt cgcgtctcag tccgaccgcc aggtgggcgc gcgggtctcg
      721 gcagggcgac ggcggctcgt aggctggccc ccatgttggc ccagagctcc gttgtcctgc
      781 cgatcatcat cggcgtcgtc gtcctcctcg tcggatcggg cgcgttcgtg gtctcgtcgc
      841 gctcgaagcg cggcgaactg gcacctccga ccaccccgaa ggccgtcacc acgactgacc
      901 gcccgaccga gctgttgccg tcggaggtcg cccccgccga ggccgatgtc gacagcgatg
      961 tcgagggcga cgtcgaggcg ccgccgtcgc tttcggagga gctcgacgcg ttggagggca
     1021 tcgaggcagc ggagaccgca gccgacaccg ccgtcgagaa ggcgacgttc cgcaaccggc
     1081 tgagccgcgc ccgcggcgcc ttgagcggtg cgctcgggtc gatccggggg cggtccgcga
     1141 tcgacgacga gacgtgggac gacctcgagg aggcgctcat ccgagccgac gtcggcgtcg
     1201 gaccgaccac ggagctgctc gattcggtgc gggcgacggt gaagcagaac ggcatcacca
     1261 cccccgacga gttgatcgac gcggtgaagg ccgagatgac gacccgcctg ctcgctccga
     1321 tcgatctgcg ggagtcggcg aacaagccca gcatctggct gttcgtcggg gtcaacgggg
     1381 tcggcaagac gacgaccatc ggcaaggtcg gcaagctcct gaccgacgac ggccgaaagg
     1441 tgctgatggc agccggcgac acgttccgcg ccgccgctgc cgagcagctg gggacgtggg
     1501 ccgagcgatg cggcgccgat ctggtgcggg gcgccgaggg cagcgacccg tcgtcggtga
     1561 tcttcgacgg catcgcccgc ggtgctgcga cgggcgccga cgtcgtgctg gccgacactg
     1621 ccggccgact ccacaccaag tccaacctca tggacgagct gcgcaaggtg cgccgtgtgg
     1681 ccgacaaggg cgagggtgag gtgaccgagg tgctcttggt gctcgacgcc acgaccgggc
     1741 agaacggtct catccaggcc cggcagttca ccgacgccgt cgaactgacc ggcgtggtgc
     1801 tgacgaagct cgacggttcc gccaaggggg gcatcgtctt cgcgatccag agcgagctcg
     1861 gcatccccgt caagctggtc ggtctcggcg aacaggcgga ggacctgatc cccttcgacg
     1921 ccgagcagtt cgtcgacgcg ctgttcgcca ccgacgaacc ctgacgcccg cccgttctgt
     1981 cccgcgaagg gattcggact gcacgccttc gcgggacaga accggtggcg gcggtggtgg
     2041 tccgggaccc tctaacctgc tcaccgcatg ttcgaagcac tgtcagaccg attcgaaggt
     2101 attttcaagc gtctccgcaa cagcggcagc ctcacgcccg accaggtcga cgaggtgctc
     2161 cgtgagatcc gggtggcgct gctcgaggcc gacgtcaacg tccgggtggt ccgcaacctc
     2221 cagaaccgca tccgggagcg ggcggttggt ctcgaggtca gcgctgcgct gacctcggcc
     2281 cagcaggtca tcaagatcgt caacgaggag ctcaccgcca ccctcggcgg cgaaacgatg
     2341 cgcatcacct tcgcgtcgaa gccgcccacg gtggtgctca tggccggcct gcaggggtcc
     2401 ggcaagacga ccaactcggc caagctggcc cgctggttca aggcgcaggg ccgcaaccca
     2461 ctgctcgtcg gcgccgacct ccagcgtcct gccgccgtcg agcagctccg ggtgctcggc
     2521 gcgtccatcg acgtccccgt ctactccgag ccgaccgacc cggtggccgt cgcccagggg
     2581 gccgtggccg aagcccggcg cctcggtcgc gacgtcgtca tcgtcgacac cgccggccgc
     2641 ctcgcgatcg acgaggagat gatggcgcag gtccgcgaca tctcggccgc cgtcgacccc
     2701 gactacacgt tcctcgtcat cgatgcgatg ttgggccagg acgccgtcac cgtcgcggag
     2761 ggcttccaca acagcctggc gctcgacggc gtcatcctca ccaagctcga cggcgacgcc
     2821 cgcggcggcg ccgcactgtc ggtcaaggag gtcgtgggtc gccccatcgc cttcgcgtcg
     2881 accggcgaga agctcgagga cttcgacctc ttccatcccg accgcctcgc cggtcgaatc
     2941 ctgggcatgg gcgacatgtt gtcgctcatc gagaaggccg aggaggcctt cgacgccgag
     3001 cagtccgagg tcgctgccca gcgactgatg gacggcacgt tcacgttcga cgacttcctc
     3061 gagcagatga acgccatccg gaagatgggc ccgttgtcga acatcatcgg catgctcccc
     3121 ggcatcccca aggaggtccg cgacgtcgag atcggcgaca aggaggtcgg caaggtcgag
     3181 gcgatcatcc ggtcgatgac gaccgaggaa cgcacgaacc ccgacatcat cgacacctcg
     3241 cgccgtcagc gcatcgcccg gggctcgggc accaacccca ccgacgtcgg ccagctcatc
     3301 acgcagttca aggaaatgcg caagatgatg cagcagatgg gcggcggagc catgaagcgc
     3361 gtcaagaaga agaccaagaa gggtcagaag aaggcctcga agggcaaccc gagcggccgc
     3421 acgactgcca agggcaccct cccgatcccg aagaaggaat tccggctccc cggcctcgac
     3481 ccgctggccg atcagctcga gaaggccggg atcgagatcc ctggtctcga cctcggtgac
     3541 cggaacgact aacatcaccc gtcgccgcgc ccacgtgacg cgtgcagctc tctgacaaga
     3601 cgagcaaccc ctaccgaaga gagaagaaat ggccgtcaga ctccgcctga tgcggatggg
     3661 caagaagaag cagcccacct accgaatcgt cgccgccgac gcccgttcgc ctcgcgacgg
     3721 ccggttcatc gagatcgttg gtacctacga cccccgtcgt gagccgtcgg ccatcaaggt
     3781 cgacaacgag aaggcgatcg cctggctcaa gaacggcgcg cagccgagcg agcgtgttga
     3841 gaagctgctc aagatctccg gtgcgtggga cgagttcgag gcgtccaagc ccgcgaagaa
     3901 gaccgcgaag gccgccaagt gagcgacggc accacggcgc gtgccgaggc cgtactcgcc
     3961 cacgtcgtca ccgcgatcgt cgaccacccc gacgacgtga aggtgtcgac cgacaccgat
     4021 cgtgaaccga cgcgcatcaa catcgaggtc ggcgacggcg acatgggtcg cgtcatcggt
     4081 cggcggggcc gcgtggccaa cgccattcgc gccgtcgggc gcgccgcagc agctgcggac
     4141 gacgtcgatg tcgacgtgga gttcgttgac tgacaacggt gctccggcgc ccggtctcct
     4201 cgaggtcggc cgcatcggca aggctcacgg cctcaagggt gaggtgtcgg tgcgcctcat
     4261 ctcggatcgc accgagcgcc tcgaccgggg cgcggtgctc tccaccgcac gcggcgacct
     4321 ccgggtcgcc gcttcgcgtc cccaccagga tcggtggatc gtgcagttcg acggcatctc
     4381 ggggcgtacc gaggccgagt cctggcacgg cgtggtgctc atgggcgagc cgatcgacga
     4441 cccgagcgcg ctgttcgtgc acgacgtggt cgggtgcacc gtggtcgacg ccgacggcgt
     4501 cgagcgcggc gtcgtcacgg cggtgcaggc gaacccggcg agcgacctcc tggtgctcga
     4561 cagcggcgca ttggttccca tgaacttcgt cgtgggcgac atcgacggca ccacgattca
     4621 cgtcgacgtc cccgacggcc tgttcgagct gtactcctga tggcctcggc cacctccgct
     4681 tcgctccggt acctctgcgg ggcagcctga tgcggatcga cgtgttcagc a
//
LOCUS       DAOSOA010000270         4703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_18234_length_4703_cov_5.941480, whole genome shotgun sequence.
ACCESSION   DAOSOA010000270 DAOSOA010000000
VERSION     DAOSOA010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18234_length_4703_cov_5.941480"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKY13_13795"
     CDS             complement(<1..365)
                     /locus_tag="PKY13_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81061.1"
                     /translation="MRALSGVVAGVTAVGLTAAAVVAAAAGIPAGDAVELIGTSFGVA
                     LVCALVGLLVVAPMRAGGTPGRALRVRAAVVAVTPALCLGLGSWAAARAMFVSPHDLS
                     ALGVIVCAAGVVGVIVTLLL"
     gene            complement(362..1096)
                     /locus_tag="PKY13_13800"
     CDS             complement(362..1096)
                     /locus_tag="PKY13_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT81062.1"
                     /translation="MQPDPDRILVVDDDPNVAEVLTRYLQLAGYRVEAVGDGAFALDR
                     AIADPPMLVILDLMLPGMDGLEVCRRLRAAAPTPVIMLTAKGGESDRVIGLELGADDY
                     VAKPFSAKELTARVKAVLRRAAGPLAGAVPGTPVELSDGDLVVDVPGRQVSRDGASIA
                     LTAREFELLVFLMRHPGRAFRREDLLQHVWGYSFGDSSTVTVHVRRLREKIEDDASRP
                     DRITTVWGVGYRWVGSSMTDLDRATA"
     assembly_gap    1209..1308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1309..1683
                     /locus_tag="PKY13_13805"
     CDS             <1309..1683
                     /locus_tag="PKY13_13805"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HOT81063.1"
                     /translation="VGGPAVQIGMDTPQTTAAHLDAAMTELAGSGTDAVLGMANDGGW
                     WIIGLHRADPAVFLDIPMSCSDTGMIQRRRLIDLGLTVRDVEPLTDVDSIDDALLVGS
                     LAPDTRFARALAAMSLPVGQAS"
     gene            1680..2282
                     /locus_tag="PKY13_13810"
     CDS             1680..2282
                     /locus_tag="PKY13_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT81064.1"
                     /translation="MSAPSPTTVLRSTDGDILPLDVEAWRRCADALEIELLEDLAAPV
                     IDVGCGPGRIVEAVASSGRMAMGVDTSPAASAEAGRRGAPVLTRSIFDPLPGERRWGS
                     VVVLDGNIGIGGDPHALLRRAHDLLAPSGTVIVEVGAPGTESDRLTVRVERGDHCGPW
                     FPWARVSADRIEHYLTLAGLLPDGVECRGHRWFATARRPA"
     gene            2293..3426
                     /locus_tag="PKY13_13815"
     CDS             2293..3426
                     /locus_tag="PKY13_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT81065.1"
                     /translation="MTTPAPLRWVEHRPKSRLHEPRVAALVGIALGVAFILCFGTGVL
                     SHLIQQPPGWFHWPSRPAGFYRISQGVHVTAGFVTIPLLLAKLWIVYPKLFEWPPVTS
                     VVHALERLSLLPLIGGGLFMVVSGTFNVARWYPWGFFFPIAHYWVGWITIGSLAIHLG
                     AKWTIVREQTGRRHLTDDLEPDRRRFIAGVAATGATVFLAVAGNTIGPLSALAVLAQR
                     RPGTGPQGLPVNKSARGAGVVDRAQSPDYRLVVEGRVERRLDLTLADLQALPQRTANL
                     PIACVEGWSSSGEWTGVPVQELLRRAGARPGATVAVESLQEGGLYRRSELNSDHAADA
                     DTLLALRLNGEPLHLDHGFPVRLIGPNRPGVMQTKWVGRLVVS"
     gene            3423..3875
                     /locus_tag="PKY13_13820"
     CDS             3423..3875
                     /locus_tag="PKY13_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81066.1"
                     /translation="MSDEPSTSRVAPIVGVIVGLPLLVVGVLGAVADGDRTKPFELAR
                     WVVGADLAHDLVLAPVVVGVSWLVGRIVPPVARGPVRWAAATTGVLCLIAWPFVRGYG
                     RNETVPSLLDRNYATGLAAYVAAIWFLALIWIAVRRARHSAPAADGEG"
     gene            3885..4661
                     /locus_tag="PKY13_13825"
     CDS             3885..4661
                     /locus_tag="PKY13_13825"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOT81067.1"
                     /translation="MLRLPDVPRRADIAVFGGSGFYSFLADTETVPTETPYGPPSADP
                     VVGLIDGRRVAFLPRHGHDHSVPAHRVNHRANVWAMAALGAQVLVSPFACGSLRRELV
                     PGDFVVVDQLVDRGHGRDGTFFDGPETFHLPFADPYDDDLRRQMIDHARRLGYVVHDG
                     GTVVVIPGPRFSTRAESRWHAAMGWDVVNMTQAPEIPLANEAGLRAVGLGLVTDFDAG
                     LEDDPSVPAVTQAEVFGFLESNAQRVQDLLRAALPALDLG"
BASE COUNT          624 a         1719 c         1561 g          699 t
ORIGIN      
        1 agcagcaagg tgacgatcac cccgaccacc ccggcggcgc agacgatgac gcccaaggcg
       61 ctgagatcgt ggggcgacac gaacatcgct cgcgccgcgg cccacgatcc gagcccgaga
      121 cagagcgccg gcgtgaccgc gacgaccgcg gcacggaccc gaagggcccg gcccggggtg
      181 cccccggctc gcatcggggc gaccacgagc aggccgacca gcgcacacac gagcgcaacg
      241 ccgaacgacg tgccgatcag ctcgacagcg tcaccggcag ggatgcccgc agcggcggcg
      301 acgaccgccg ccgcggtcag cccgaccgcc gtcacgccgg cgacgacgcc cgacaacgcc
      361 ctcatgccgt cgcccggtcg agatcggtca tcgaggagcc gacccaccgg tagcccacac
      421 cccacacggt cgtgatccgg tcggggcgac tcgcgtcgtc ctcgatcttc tcgcggagtc
      481 ggcggacgtg gacggtgacg gtggacgaat cgccgaacga gtacccccag acgtgctgca
      541 gcaggtcctc gcgccggaac gcccggccgg ggtggcgcat gaggaagacc agcaactcga
      601 actcgcgggc cgtcaacgcg atcgacgcac cgtcacgcga cacctgccgg ccgggcacgt
      661 cgaccacgag gtcgccgtcg ctgagctcca ctggcgtgcc gggaacggcg cccgcgagcg
      721 gaccggcggc acgccggagc acggccttga cccgagcggt gagttccttc gccgagaagg
      781 gtttggcaac gtagtcgtcg gcaccgagct cgagcccgat gacgcggtcg gactccccgc
      841 ccttggccgt gagcatgatc accggcgtcg gggccgccgc tcggagccga cggcagacct
      901 cgagtccgtc catcccgggg agcatcagat cgaggatgac gagcatcgga gggtcggcga
      961 tggcccggtc caacgcgaag gcgccgtccc ccaccgcctc gacgcggtac ccagcgagtt
     1021 gcaggtaccg cgtgagcacc tcggcgacgt tcgggtcgtc gtcgacgacg aggatccggt
     1081 ccgggtcggg ttgcatcggg tcagcgtacg cccgcgccct tcgtcccgac gtttcgacgc
     1141 gaccccccgt ccgagtgttt cgatttccgt aagcggggta gggccgtcga atggacatcc
     1201 gcatcgtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gggcggacca
     1321 gccgtacaga tcggcatgga cacaccccag acgacggcgg cgcacctcga cgcggcgatg
     1381 accgagctgg cgggctccgg caccgacgcc gtgctcggca tggccaacga cggcgggtgg
     1441 tggatcatcg gtctgcatcg cgccgacccc gccgtcttcc tcgacatccc gatgagctgc
     1501 agcgacaccg ggatgatcca acgtcgccgc ctcatcgatc tgggcctcac cgttcgagac
     1561 gtcgagccgc tcaccgacgt cgactcgatc gacgacgcgc tgctcgtcgg gtcgctcgcc
     1621 cccgacaccc gcttcgcccg cgccctcgcg gcgatgtcgc tccccgtcgg gcaggcgtcg
     1681 tgagcgcccc gtcaccgacg acggtcctgc gcagcaccga cggcgacatc ctgcccttgg
     1741 acgtcgaggc gtggcggcgg tgcgccgacg ccctcgagat cgagctcctc gaggacctcg
     1801 ccgcgcccgt tatcgacgtc gggtgtggtc ccggccgcat cgtcgaggcc gtcgcgtcga
     1861 gcggtcgaat ggccatgggc gtcgacacct cgccggcggc gagcgccgaa gccggccgcc
     1921 gcggcgcgcc cgtgctcacc cgatcgatct tcgaccccct cccgggcgag cggcgctggg
     1981 gttcggtcgt tgtgctcgac gggaacatcg gcatcggcgg cgatccgcac gcgctgcttc
     2041 ggcgggccca cgacctgctg gcaccttcgg gcacggtgat cgtggaagtc ggcgcccccg
     2101 gcacggagag cgaccggctc accgtccggg tcgaacgcgg cgatcactgc gggccgtggt
     2161 ttccctgggc ccgggtgtcc gccgaccgca tcgagcacta cctcaccctc gccgggttgc
     2221 tccccgacgg cgtcgagtgc cgcgggcatc ggtggttcgc cacggctcgg cggccggcgt
     2281 gaggtctccc tcgtgacgac cccggctccg ctccgttggg tcgaacaccg ccccaagagc
     2341 cggctccacg agccccgcgt cgccgccctc gtcggcatcg cgctcggcgt cgccttcatc
     2401 ctgtgcttcg gcaccggcgt cctcagccac ctcatccagc aacctccggg ctggttccac
     2461 tggccgtcgc gtccggccgg cttctaccgg atctcccaag gagtccacgt cacggccggc
     2521 ttcgtcacga tccccttgct gctggccaag ttgtggatcg tgtaccccaa gttgttcgag
     2581 tggccgcccg tcacctccgt ggtccatgcg ctggaacgac tgagcctgct gccgctgatc
     2641 ggcggcggtc tcttcatggt cgtgtcgggc acgttcaacg tcgcccgctg gtatccctgg
     2701 ggtttcttct tcccgatcgc ccactactgg gtgggatgga tcacgatcgg ctcgctggcg
     2761 atccatctgg gtgcgaagtg gacgatcgtg cgcgagcaga cgggacgtcg ccacctcacc
     2821 gacgacctcg agccggaccg ccggcggttc atcgccggcg tcgcagccac gggagcgacc
     2881 gtgttcctcg cggtcgccgg gaacacgatc gggccgttgt ctgccctcgc ggtccttgcg
     2941 cagcgacgtc cgggcaccgg cccgcagggc cttcccgtca acaagtcggc gcggggcgcg
     3001 ggcgtcgtcg accgagcgca gtcccccgac taccgcctgg tggtggaggg ccgagtggaa
     3061 cgccgcctcg acctcaccct cgccgatctg caggcgctcc cacagcggac cgcgaacctt
     3121 ccgatcgcct gcgtcgaggg ctggagttcg tcgggcgagt ggaccggtgt acccgtccag
     3181 gagttgctgc ggcgtgccgg cgcccgcccg ggggccacgg tcgcggtcga gtcgctccag
     3241 gagggcggcc tctatcgccg atccgaactc aacagcgacc acgctgccga tgccgacacg
     3301 ctcctcgcac tccggttgaa cggtgaacct ctccatctcg accacgggtt ccccgtccgg
     3361 ctcatcggcc cgaaccgacc cggcgtgatg cagacgaagt gggtcgggcg gctggtggtg
     3421 tcatgagcga cgagccctcc acgtcacggg tggcacccat cgtcggggtg atcgtcgggc
     3481 ttccgctgct cgtcgtcgga gtcctcggcg ccgtcgccga cggcgaccgc accaaaccct
     3541 tcgagctcgc ccgatgggtc gtcggcgcgg acctcgccca cgacctcgtg ctcgcccccg
     3601 tcgtcgtcgg ggtgagctgg ctcgtcgggc ggatcgttcc gcccgttgcc cgggggcccg
     3661 tccggtgggc ggccgcgacg accggcgtcc tgtgcctcat cgcgtggccg ttcgtccggg
     3721 gctacggccg caacgagacc gtgccgtcgc tcctcgaccg caactacgcg accggactcg
     3781 ccgcctacgt cgctgccatc tggttcctgg cgctcatctg gatcgccgtt cgccgggctc
     3841 gccacagcgc accggccgcc gacggcgagg gctaggttcg ccggatgctg cgcctccccg
     3901 acgtcccccg acgcgccgac atcgccgtgt tcggcgggtc cggcttctac tcgttcctcg
     3961 ccgacaccga gaccgtgccg accgagacgc cgtacggtcc gccgtccgcc gaccccgtcg
     4021 tcgggctgat cgacggccgt cgggtcgcgt tcctcccccg gcacggccac gaccattcgg
     4081 taccggccca ccgggtcaac caccgcgcca acgtgtgggc gatggcggcg ctgggcgctc
     4141 aggtgctcgt ctcgccgttc gcgtgcggat cgttacgacg cgagctggtg cccggcgact
     4201 tcgtggtcgt cgaccagctc gtcgatcggg gccacggtcg cgacgggacg ttcttcgacg
     4261 gtcccgagac gttccacctc cccttcgccg acccctacga cgacgatctc cgtcggcaga
     4321 tgatcgacca cgcccggcgc ctcgggtacg tcgtgcacga cggtgggacc gtcgtcgtca
     4381 ttcccggccc acggttctcc acccgggcgg aatcgcgttg gcacgcggcc atgggctggg
     4441 acgttgtgaa catgacacag gcgcccgaga tcccgctggc gaacgaggcc ggcctccggg
     4501 cggtcggcct gggcctggtg accgacttcg atgccggcct cgaggacgat ccgtcggtgc
     4561 cggcggtgac ccaggccgag gtcttcggct tcctcgagtc caatgcacag cgtgtgcagg
     4621 acctgctccg agcggcactc cccgcgctcg acctcggctg actcgactga cccgttgtca
     4681 gttggtgggg gtgtcgatgg gtg
//
LOCUS       DAOSOA010000271         4690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_18302_length_4690_cov_8.134628, whole genome shotgun sequence.
ACCESSION   DAOSOA010000271 DAOSOA010000000
VERSION     DAOSOA010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18302_length_4690_cov_8.134628"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1570
                     /gene="purB"
                     /locus_tag="PKY13_13830"
     CDS             110..1570
                     /gene="purB"
                     /locus_tag="PKY13_13830"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOT81068.1"
                     /translation="MTTADSAGAPVAKSAIPNVLASRYASTAMVDLWSPRRKIVLERQ
                     LWIAVMTAQRELGIDIPDGVIADYEAVVDEVDLASIAERERVTRHDVKARIDEFCALA
                     GHEHIHKGMTSRDLTENVEQLQIRLALLLVRDRMVTTLARLAQRAAEFSTQPLTGRSH
                     NVAAQTTTLGKRFANAGQELLVAFERIEQLIERYPLRGLKGPVGTQQDQLDLLAGDTA
                     AMDDLERRVAAHLGFEAVLTNVGQVYPRSLDFDVVSALVQAAAAPASLATTLRLMAGH
                     DLATEGFRPGQVGSSAMPHKMNSRSCERICGFHTILGGYLVMVNGLAGDQWNEGDVSC
                     SVVRRVALPDAFFAIDGVFETTLTVLDEFGAYPAVIQRELDRYLPFLATTKVLMAAVR
                     AGVGRETAHEVIKEHAVSAALGMREEAAERNDLIDRLEADSRLGMEPGSLARLLADPL
                     SFTGNAVDQTAAFVAAVEAIAERHPAAAAYSPAPIL"
     gene            1632..2519
                     /locus_tag="PKY13_13835"
     CDS             1632..2519
                     /locus_tag="PKY13_13835"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT81069.1"
                     /translation="MTELGLPHIYSGKVRDIYDAGDGRLLMVTSDRMSAFDVVMDEPV
                     PDKGRVLTAMSAFWFEQFADEVGSHLISTDLADLPESAQQPEIAGRFMLCRRAEMLPV
                     ECIVRGYITGSAWKEYRNHGTMHGAVLPEGLQESDRLPEAVFTPSTKAEVGDHDENIS
                     FERAVELIGEDLAVRARDLSLALYARGAELAAERGIIIADTKFEMGLVDGELVLADEV
                     LTPDSSRFWPADDWVPGTTPPSFDKQPIRDYLETLDWNKQAPPPPLPDEVVEASSVRY
                     REAYERITGRSLADWPGAS"
     gene            2560..2814
                     /gene="purS"
                     /locus_tag="PKY13_13840"
     CDS             2560..2814
                     /gene="purS"
                     /locus_tag="PKY13_13840"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOT81070.1"
                     /translation="MIFDCHVDVQLRPGIADPQGSTIEKALPALGFEGVAGVRVGKSV
                     KFQVEAPDEAAARSQIEDICDRFLTNPVIEDATISVSASS"
     gene            2816..3484
                     /gene="purQ"
                     /locus_tag="PKY13_13845"
     CDS             2816..3484
                     /gene="purQ"
                     /locus_tag="PKY13_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOT81071.1"
                     /translation="MATTIGVVQFPGSNCELDVIEAVEALGATAEILWHDDADLRGVD
                     AVVIPGGFAHGDYLRPGAIARFSPVMGSVATFAADGGPVVGICNGFQVLTEAHLLPGA
                     LQKNAGLKFRCGPAEIRVETVDSVLTSGASVGAVLSIPINHFEGNYTCSPETLAELRA
                     EDRVVVRYVDNPNGSTDDIAGVCSAGRNVVGLMPHPERASHELLGSTDGVVLFRSLLS
                     AAGV"
     gene            complement(3612..4019)
                     /locus_tag="PKY13_13850"
     CDS             complement(3612..4019)
                     /locus_tag="PKY13_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81072.1"
                     /translation="MAEAKTRGMSDQHKEALATGRNQSRAVKAYLAALKSNKPKRGRK
                     RTAESISNRLAKIANELPTADALQEVLLLQEQVDLEKELQTIGNDVDLSELEAEFIKV
                     GLGYSNSKGIDYSTWRKVGVSAETLAKAGIKRS"
     gene            4309..>4690
                     /locus_tag="PKY13_13855"
     CDS             4309..>4690
                     /locus_tag="PKY13_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOT81073.1"
                     /translation="MADTPDVPVHRALGLTDEEYADIATILGRQPNHLELAMYSVMWS
                     EHCSYKSSRVHLGRFPTEAPWVMVGPGENAGVVDVGDGIALAMRIESHNHPSAIEPYQ
                     GAATGIGGILRDIFTMGARPIALMD"
BASE COUNT          722 a         1611 c         1546 g          811 t
ORIGIN      
        1 ggctcgatga acgcagcgct gctcgtcgtg cagatgctgg cgatcaccga cgccgacctg
       61 gcggcgaagc ttgccgacca ccgtcgcgag atggcgggct gatccgccga tgacgactgc
      121 cgacagcgcc ggcgcccctg tggcgaagtc ggccattccc aacgtcctcg ccagccggta
      181 tgcctcgacg gcgatggtcg acctctggtc gcctcgccgc aagatcgtgc tggaacggca
      241 gctgtggatc gcggtgatga cggcccaacg cgaactcggc atcgacatcc ccgacggggt
      301 catcgccgac tacgaggcgg tcgtcgacga ggtcgacctg gcgtcgatcg ccgagcggga
      361 gcgggtgacc cgccatgacg tgaaagcgcg catcgacgag ttctgcgccc tcgccggtca
      421 cgaacacatc cacaagggca tgacgtcgcg tgacctcacg gagaacgtcg aacagctcca
      481 gatccgtctg gccctgctcc tcgtccgcga ccgtatggtc accacgttgg cgcggctggc
      541 ccagcgcgct gcggagttct cgacccagcc gctcacgggg cgcagccaca acgtggcggc
      601 gcagacgacg acgctcggca agcgattcgc caacgccggg caggaactgc tcgtcgcgtt
      661 cgagcggatc gagcagctca tcgagcgtta cccgctccgt gggctgaagg gcccggtcgg
      721 cacgcagcag gatcagctcg atctgctcgc gggcgacacc gcggcgatgg acgacctcga
      781 acgacgcgtc gccgcccacc tcgggttcga ggccgtgctc accaacgtcg gtcaggtcta
      841 cccgcggtcg ctcgacttcg acgtcgtgtc ggcgttggtc caggccgcgg cggcgccggc
      901 ctcgttggcg accacgcttc gcctgatggc gggccacgat cttgcgaccg agggattccg
      961 ccccgggcag gtcggcagtt cggccatgcc gcacaagatg aacagccgca gttgcgagcg
     1021 catctgcggc ttccacacga tcctcggtgg gtacctcgtg atggtcaacg gcctcgccgg
     1081 tgaccagtgg aacgagggcg acgtgagttg ctcggtcgtc cggcgggtcg cgctgcccga
     1141 cgccttcttc gcgatcgacg gcgtgttcga gacgacgctg acggtgctcg acgagttcgg
     1201 tgcctacccg gcggtgatcc agcgcgaact cgaccgctat ctcccgttcc tcgcgacgac
     1261 caaggtgctc atggccgcgg tccgtgccgg cgtcggccgc gagacggcgc acgaggtgat
     1321 caaggagcac gccgtgtcag cggccctcgg catgcgggag gaggcggctg agcgcaacga
     1381 cctcatcgac cgactcgagg ccgactcccg tctgggcatg gagcccggct cgctcgcccg
     1441 gctgctcgcc gacccgctct cgttcaccgg caacgccgtc gaccagacgg cagcgttcgt
     1501 cgcagcggtc gaagccatcg ccgagcgtca ccccgcggcg gcggcctact cgccggcgcc
     1561 catcctctga tccgtccacc gattccctcg cgccggcccg ccctgcgcat ccccgacaga
     1621 aaggtgcacc catgaccgaa ctcgggctcc cgcacatcta ctccggcaag gtccgcgaca
     1681 tctacgacgc cggcgacggc cggctgctga tggtcacctc cgaccgcatg tcggcgttcg
     1741 acgtggtcat ggacgagccg gttccggaca agggccgggt gttgaccgcc atgtcggcgt
     1801 tctggttcga gcagttcgcc gacgaggtcg gttcccacct gatctccacc gacctcgccg
     1861 acctgccgga gtcggcccag cagcccgaga tcgccggtcg cttcatgttg tgtcgtcgcg
     1921 ccgagatgtt gcccgtcgag tgcattgtcc gcggctacat caccgggtcg gcgtggaagg
     1981 agtaccgcaa ccacggcacc atgcacggcg ctgtactgcc ggagggattg caggaatcgg
     2041 atcggcttcc cgaggcggtg ttcacgccgt cgaccaaggc cgaggtcggc gaccatgacg
     2101 agaacatcag cttcgaacgc gccgtcgaac tgatcggcga ggacctcgcc gtgcgtgccc
     2161 gcgacctgtc cctcgccctg tatgcacggg gcgccgagct cgctgccgag cgaggcatca
     2221 tcatcgccga caccaagttc gagatgggtc tggtggacgg tgagctggtg ctcgccgacg
     2281 aagtcctcac ccccgactca tcacgcttct ggccggccga cgactgggtg ccgggcacga
     2341 cgcccccgag cttcgacaag cagccgatcc gtgactatct cgagacgctc gactggaaca
     2401 agcaggcgcc gccgccgccg cttcccgacg aggtcgtcga ggcgtcgtcg gtccggtatc
     2461 gcgaggccta cgagcgcatc accggccgtt ccctcgccga ctggccggga gcgtcgtgag
     2521 cgtcgggtgc tcctccgaga atgtgaaagg cttgacctca tgatcttcga ctgtcacgtg
     2581 gacgtgcagc tccgccccgg aatcgccgat ccccagggct ccaccatcga gaaggcgctg
     2641 cccgcgctcg gattcgaggg ggtggccggg gtgcgggtcg gcaagagcgt gaagttccag
     2701 gtcgaagccc ccgacgaggc ggctgcgcgc agccagatcg aggacatctg cgaccggttc
     2761 ctcaccaacc ccgtgatcga ggacgcgacc atcagcgtca gcgcgagtag ctgaggtggc
     2821 cacgacgatc ggcgtcgtcc agttccccgg ctcgaactgc gagctcgacg tcatcgaagc
     2881 agtggaggca ctcggcgcca ccgccgagat cctgtggcac gatgacgccg acctgcgtgg
     2941 cgtcgacgcc gtcgtgatcc ccggcgggtt cgcccacggc gactacctac gcccgggtgc
     3001 cattgcgcgt ttctccccgg tcatggggtc cgtcgcgacc tttgccgccg acggtgggcc
     3061 ggtcgtcggc atctgcaacg ggttccaggt gctcaccgag gcgcacctgc tgccgggtgc
     3121 gctccagaag aacgccgggc tcaagttccg gtgcggccct gcggagatcc gggtcgagac
     3181 cgtcgactcc gtgctcacgt cgggagcgtc ggtcggcgcc gtgctcagca tcccgatcaa
     3241 tcacttcgag ggcaactaca cctgctcgcc cgagacgctg gcggagctgc gcgccgagga
     3301 ccgtgttgtg gtccgctacg tcgacaaccc caacggttcg accgacgaca tcgccggcgt
     3361 ctgctcggcg gggcgcaacg tcgtcgggct catgccgcat ccggagcggg cgtcccacga
     3421 gctgctcggg tccaccgacg gtgtggtgtt gttccgttcg ctcctcagtg ccgccggcgt
     3481 ctgagactcg tggcgcagcc gtctgagacg gggccgccgg cgtctgaagc gccgacccgg
     3541 acgtccgctg gctgggttcc cccgacgagt tcgcgcactc gcccgtcggg ggacctccac
     3601 gcgttcggtt cctaagagcg cttgataccg gctttggcga gggtctcggc gctgacgccg
     3661 accttgcgcc atgtcgagta gtcgatgccc ttcgagttgg aatatccgag gccgaccttg
     3721 atgaattcgg cttcgagctc gctgagatcg acgtcattgc cgatcgtctg cagttccttc
     3781 tcgagatcga cctgctcttg gagcagcagc acttcctgca acgcatcagc ggtcgggagc
     3841 tcgttggcga tcttggcgag tcggttgctg atcgactcgg cggtgcgctt gcggccacgc
     3901 ttgggcttgt tgctcttcag ggcggcgagg tacgccttca cggcccggct ctggttgcgc
     3961 ccggtggcga gcgcctcctt gtgctggtcg ctcattcctc gggttttggc ttcagccatt
     4021 tcttctcacc ttttcgtcgg ttccatgtgc acacatatcg tcaccgcaac acaatggggg
     4081 acacgcccgg ggaaatcagg ggcatggatc atgtcgcagt ggcatcgata ttacacagcc
     4141 catcatcaat gacttggcac cccattggtc agtatcgatt tattcagatg tgattgggtg
     4201 atttacctcc tggcgcggtg ggtcgaatcg cacgtcggcg ttcgatcggg cggtgagggc
     4261 ggcccatcgt cggttcaggc gctccggcgc ggccactagt ttggagccat ggccgacacc
     4321 ccagacgtcc cggtccaccg ggcccttggc ttgaccgacg aggagtacgc cgacattgcg
     4381 acgatcctcg gacgtcagcc caaccacctc gagctcgcga tgtactcggt catgtggtcg
     4441 gagcactgct cctacaaatc gtcccgggtc cacctcgggc gattccccac cgaggcaccg
     4501 tgggtgatgg tcggtccggg cgagaacgcc ggtgtggtcg acgtcggtga cggcatcgcg
     4561 ttggcgatgc gcatcgagag ccacaaccac ccgtccgcga tcgagccgta ccagggtgcg
     4621 gcgacgggca tcggcggcat cctccgcgac atcttcacca tgggcgcccg cccgattgcg
     4681 ctcatggacc
//
LOCUS       DAOSOA010000272         4678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_18360_length_4678_cov_5.903093, whole genome shotgun sequence.
ACCESSION   DAOSOA010000272 DAOSOA010000000
VERSION     DAOSOA010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18360_length_4678_cov_5.903093"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PKY13_13860"
     CDS             complement(<1..952)
                     /locus_tag="PKY13_13860"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT81074.1"
                     /translation="MRDWLNLLADPGSAVPIPGTDESVAAARCRIDGRELVLVWCRFD
                     VLAGTLSVAAGERFVAAVDHAVEARLPVLGIANSGGARMQEGSRAFVQMIAATRAVRR
                     LRDAGLPLVVYTANPTTGGVFASWASLGHLTFAEPGATIGFTGPRVAQALGEPIVPAS
                     SQTAEGVLANGHVDAVVAPDDLRSTMAQVLATLAPATPAGPLSGPPSAAPAEAVEGPL
                     GWAAVQGSRGSTHRRVLDDLLALATSVIELVGDRAGSVGSQSTCVIARIDGRPVVVVG
                     HRPGARPTAADLRAARRAMTIAQELGLPVVTVIDTPGAEIS"
     gene            complement(957..2816)
                     /locus_tag="PKY13_13865"
     CDS             complement(957..2816)
                     /locus_tag="PKY13_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT81075.1"
                     /translation="MASSRAPEGMSASEPGVDRHELNLIFSARHVVILTFQILSYFGR
                     DRALDWWEPAIDLGIVLPYSAVTHWVALRKLRMPGWVPIVDSLVIVAWVVAMPETSPT
                     LVAMLVAMLTIAGQSVARRWLWTAFGLSSAGMLASALLANELSNFLDAARSIVLGACV
                     LMTVTMVRTARLEAQRRLAYTAQHDQLTGLANRSLLHHEIGAVGTDRDLAVLLADLNN
                     FKEVNDALGHHVGDDLLVAVSRRVVDVAPAAATVGRLGGDEFAVVIPDSNRADAIGAA
                     DRITAALRQPFTVDGLTIEVGASIGIALRNPGAPAPVPSDVLFRNADVAMYQAKQAGR
                     PFRVYDPSDDHSSVRRVTLMGELRTAIADGQLEVWYQPGLDAHTGQLNCMEALVRWNH
                     PQHGLLLPGEFIELAEISGAIDDLTQWVIDRAVRDSHRLSALGRPLLVSCNLSVRNLH
                     DRLLLEWLDELATTVGLPDSGLYLELTESQIMEDPAGASHVLTRLAEMGIGSAVDDFG
                     TGYSSLSMLLHVRASVLKIDRSFVADLLRTREASVMVRSMIDLAHNLGMLVVAEGVED
                     QMTLERLEAMGCDFLQGFHIARPMPFDQLTGFLGLAPADGIDGVSPVIGAALS"
     gene            complement(2920..4473)
                     /locus_tag="PKY13_13870"
     CDS             complement(2920..4473)
                     /locus_tag="PKY13_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HOT81076.1"
                     /translation="MNGARALIQTLVDSGVDVCFTNPGTSEMHFVAALDEVEPMRGIL
                     CLFEGGATGAADGYARMADKPAGTLLHLGPGLGNGFANLHNARRARTPVVNIVGDHAT
                     YHKQYDAPLESDIDAVAQACSGWVRRSTTPDEVAADAAATVAASLASPNQVATLILPA
                     DVCWLDAEGPHQPIDPEPARVPTPELVAELVDAFAGGSSTVLLIGGRLMRERGLVAAA
                     RIAAATGVRVLCETFPTRQERGAGLPTVDRLAYLAEFAQMQLDGAQHLVVVDTAAPVS
                     FFAYPSKASVLHPDGCIVHEVDLDIDPVDLLEAAAAALGAPDEVPVAAAARPELPTGP
                     LTSETVAQALGALMPENAVVVDEANTSGLFIPGATAGAPRHDWLCLTGGAIGIGIPLA
                     TGAAVACPDRQVLCLEADGSAMYTLQALWTQVREQLNVITVIFANHSYAVLNMELNRV
                     GAASVGPKALKMLDLTGPTLDFVSLAQGMGVEASRATTSDEFVEQLQAALAAGGPRLI
                     EAVVPPLGL"
BASE COUNT          736 a         1531 c         1765 g          646 t
ORIGIN      
        1 cgctgatctc ggcgccgggg gtgtcgatga ccgtcaccac cggcagcccg agttcctggg
       61 cgatcgtcat cgcccgacgg gcggcgcgca ggtcggccgc cgtcgggcgc gcaccgggtc
      121 gatggcccac gacgacgacg ggtcgtccgt cgatgcgggc gatcacacac gtcgactgcg
      181 agccgaccga tccggcccga tcgccgacga gttcgatcac gctcgtcgcc agtgcgagca
      241 ggtcgtcgag cacacgccga tgggtcgagc cccgcgagcc ctggaccgcc gcccatccca
      301 gcggcccctc gaccgcttcg gccggtgctg cggacggtgg accggagagc ggaccggccg
      361 gggtggccgg tgccagcgtg gcgagcacct gcgccatcgt cgaccgcagg tcgtcgggcg
      421 ccaccacggc gtcgacgtgg ccgttggcga gcaccccctc ggccgtctgc gacgaggccg
      481 gcacgatcgg ctcgccgagg gcctgggcca cccgggggcc ggtgaatccg atggtggcac
      541 cgggttcggc gaacgtcagg tgcccgagcg acgcccacga cgcgaagaca ccgcccgtgg
      601 tcgggttcgc ggtgtagacg accaggggga gtccggcgtc acgcagccga cgcacggcac
      661 gcgtcgccgc gatcatctgg acgaacgcgc gcgatccttc ctgcatgcgt gcgccacccg
      721 agttggcgat gccgagcacg ggaagccgcg cctccaccgc gtggtcgacg gcagcgacga
      781 accgctcccc tgccgccacg ctgagggtgc ccgccaacac gtcgaagcgg caccagacga
      841 gcaccagttc ccggccgtcg atccgacacc gcgccgctgc aacggattcg tcggtaccag
      901 ggatcggaac cgccgaaccc gggtccgcca gcaggttcaa ccagtcgcgc accgggtcac
      961 gatagggcgg ccccgatgac cggtgaaact ccgtcgatcc cgtcggcggg cgccagaccg
     1021 aggaagccgg tcagctggtc gaacggcatc ggacgggcga tgtggaagcc ctgcaggaag
     1081 tcacacccca ttgcctccag ccgctccaac gtcatctggt cctcgacccc ttcggcgacg
     1141 accagcatcc cgaggttgtg ggcgaggtcg atcatcgacc gaaccatgac gctggcctcg
     1201 cgggtcctga ggagatccgc gacgaacgac cggtcgatct tgaggaccga ggcccgcacg
     1261 tgcagcagca tcgacagcga cgagtacccg gtcccgaagt cgtcgacggc cgaaccgatg
     1321 cccatctccg cgagacgcgt cagcacgtgc gatgccccgg cggggtcctc catgatctgg
     1381 ctctcggtca gctcgaggta caggccgctg tcgggcagcc cgacggtggt ggcgagttcg
     1441 tcgagccact cgaggagcaa ccggtcgtgc aggttgcgca ccgacaggtt gcacgacacg
     1501 aggagcgggc gacccagagc cgagagacgg tgggagtcgc gcaccgcccg gtcgatcacc
     1561 cactgggtga ggtcgtcgat cgcaccggag atctcggcca gctcgatgaa ctcgccgggg
     1621 agcaggagcc cgtgttgggg atggttccag cgaacgagcg cttccatgca gttcaactgc
     1681 ccggtgtgcg cgtcgagtcc cggctggtac cagacctcca actggccgtc ggcgatcgcc
     1741 gtacgcagct cacccatcaa cgtgacgcga cggacgctcg aatggtcgtc ggaggggtcg
     1801 tagacgcgga acgggcggcc ggcctgcttc gcctggtaca tcgccacgtc ggcattgcgg
     1861 aacaggacgt ccgacgggac cggcgccggc gctccggggt tccgcagcgc gatgccgatg
     1921 ctggcaccga cctcgatggt cagtccgtcc accgtgaacg gctgtcgcaa ggccgcggtg
     1981 atccggtcgg cggccccgat cgcgtccgcc cggttcgagt cggggatcac caccgcgaac
     2041 tcgtcgccac ccagacgacc gaccgttgcc gcagcgggcg cgacgtcgac gacgcggcga
     2101 ctgaccgcca cgagcagatc gtcaccgacg tggtgaccga gcgcgtcgtt gacctccttg
     2161 aagttgttga ggtcagcgag gagcacggcg aggtcacgat ccgtgccgac cgcgccgatc
     2221 tcgtggtgaa gaagggaccg attggcgagc ccggtcagct ggtcgtgctg ggcggtgtag
     2281 gcgaggcgac gctgcgcttc gagacgagcc gtacgcacca tcgtcaccgt catcagcaca
     2341 cacgccccca agacgatgct ccgggcagcg tcgaggaagt tggacaactc gttcgccaga
     2401 agcgccgacg cgagcatgcc ggcagagctg aggccgaacg ccgtccagag ccagcgccgg
     2461 gcgaccgact ggccggcgat cgtcaacatc gcgacgagca tcgcgacgag cgtcggggag
     2521 gtctcgggca tcgccaccac ccaggcgacg atgaccaacg aatccacgat cggcacccac
     2581 cccggcattc gcaacttccg cagcgcgacc caatgggtga cggcgctgta gggcagcacg
     2641 atcccgagat cgatggccgg ttcccaccag tcgagggccc ggtcgcggcc gaagtaggag
     2701 aggatctgga acgtcaggat gacaacgtgg cgcgccgaga agatcaggtt cagttcgtgg
     2761 cggtcgacgc ccggttcgga cgcggacatc ccctccggag cgcgtgacga ggccatgagg
     2821 tggtgatcgg tcgcgtcgcg gcgctggttg accttcccca cgggcgatgg gccgaacggc
     2881 ccgggtccga tgacccgagt cgtcggcggc gtgtggctgt cagaggccga gcggaggaac
     2941 cacggcctcg atgaggcgcg gcccgccggc ggcgagcgct gcctgcagct gctcgacgaa
     3001 ctcgtcgctg gtcgtggcgc gggacgcctc gacccccatg ccctgggcga gcgacacgaa
     3061 gtcgagcgtg ggcccggtga ggtcgagcat cttgagtgcc ttgggtccga cgctggcggc
     3121 gccgacgcgg ttgagttcca tgttgaggac cgcgtagctg tggttggcga agatgaccgt
     3181 gatgacgttc agctgctcgc ggacctgggt ccagagcgcc tgcagcgtgt acatcgccga
     3241 cccgtcggct tcgaggcaca gcacctggcg gtcggggcag gccacggcgg cgccggtggc
     3301 gagcgggatg ccgatgccga tggcaccgcc ggtgaggcac agccagtcgt ggcggggcgc
     3361 accggcggtc gcgccgggga tgaacaggcc cgaggtgttg gcctcgtcga cgaccacggc
     3421 gttctcgggc atgagggcgc cgagggcctg tgcgacggtc tcggaggtga gcgggccggt
     3481 cggcagctcg gggcgggcgg cggcagcgac gggaacctcg tcgggcgcgc cgagcgctgc
     3541 ggcggcggct tcgaggaggt cgacggggtc gatgtcgagg tcgacctcgt ggacgatgca
     3601 cccgtcgggg tgcaggaccg acgccttgga cggataggcg aagaacgaca ccggcgccgc
     3661 ggtgtcgacc acaacgaggt gctgggcgcc gtcgagctgc atctgggcga actcggcgag
     3721 gtaggcgagg cggtcgacgg tgggcaggcc ggcgccgcgc tcctggcggg tggggaaggt
     3781 ctcgcagagc acgcggaccc cggtcgccgc ggcgatccgg gcggcggcca cgaggccccg
     3841 ctcccgcatc aggcggccgc cgatgagcag caccgtcgat gaaccgccgg cgaaggcgtc
     3901 gacgagttcg gccaccagtt cgggggtcgg cacgcgggcc ggctccggat cgatcggctg
     3961 atgcggtccc tcggcatcga gccagcagac gtcggcgggc aggatcagcg tggccacctg
     4021 gttgggagag gcgagcgagg ccgcgacggt ggcggcggcg tcggcagcga cctcgtcggg
     4081 cgtggtggaa cggcggaccc acccggagca tgcctgggcg acggcatcga tgtcggactc
     4141 gaggggcgcg tcgtactgct tgtggtacgt ggcgtggtca cccacgatgt tgacgacggg
     4201 ggtgcgggcg cgccgggcgt tgtggaggtt ggcgaagccg ttgccgaggc cggggccgag
     4261 gtgcaggagc gtgccggccg gcttgtcggc catccgggcg tagccgtcgg ccgcaccggt
     4321 ggcgccaccc tcgaacaggc acaggatgcc ccgcatgggc tcgacctcgt cgagcgcggc
     4381 gacgaagtgc atctccgacg tgccggggtt ggtgaagcag acgtcgacgc cggagtcgac
     4441 gagggtctgg atgagtgcgc gtgcgccgtt catgtgcggg tgttctccgg ggttgctctg
     4501 gttttgcggg tagttgtcgt cagcaggtga cgacaactac ccgcagaacc ggtgggatcg
     4561 ggtgaggtcg agtgggtggg cggggtggcg gggtggcggc cgctagaaga cggcgccagg
     4621 gttcaggatg ccgttggggt cgaatgcggt cttgatgcgg cgcatcaggt cgatcttg
//
LOCUS       DAOSOA010000273         4607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_18750_length_4607_cov_5.133568, whole genome shotgun sequence.
ACCESSION   DAOSOA010000273 DAOSOA010000000
VERSION     DAOSOA010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18750_length_4607_cov_5.133568"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKY13_13875"
     CDS             complement(<1..470)
                     /locus_tag="PKY13_13875"
                     /inference="COORDINATES: protein motif:HMM:NF020544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HOT81077.1"
                     /translation="MAGRGVLFAIGDGDVAALLGADGHAEVVKYVKGTIQERWEKDFV
                     AETDKAWYAIHMCVAGSAALDESEAPESRAIFGSATLTTEDDRTVAFTPADEVAATAA
                     ALAAIDSDALRAQYDLIDPETYAWADLSDDDFKYTWVYFEAARNLWQKAADAGRA"
     gene            complement(482..1042)
                     /locus_tag="PKY13_13880"
     CDS             complement(482..1042)
                     /locus_tag="PKY13_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOT81078.1"
                     /translation="MIIVVSGPGGAGKGTVVAAWRERDPKLYVNRSWTTRERRPGEAE
                     DAYVFATWDEFQRRIDDGGFLEWVDFLDYRQGSPVPEPPDGHDVIFEIDVYGAKAVKE
                     KFPDALCIYLEAPSRDVQRERLVHRGDTPERVEQRVARADEEAAAAKDLDSVLVVNHE
                     VSDTVDHLAALVDERRRAARASSEPD"
     gene            complement(1039..1362)
                     /locus_tag="PKY13_13885"
     CDS             complement(1039..1362)
                     /locus_tag="PKY13_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81079.1"
                     /translation="MTTVVLHDALEWAARTRAFRERFVGRCSAGLSLADVRAELERER
                     HVLPAREVDRLAELRLLPVLEAIPAVGGKVASRRLMAAAGVPEDIELGAIPEAAWSVL
                     LGERA"
     gene            complement(1359..2063)
                     /gene="pyrF"
                     /locus_tag="PKY13_13890"
     CDS             complement(1359..2063)
                     /gene="pyrF"
                     /locus_tag="PKY13_13890"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT81080.1"
                     /translation="MADIAGEVSEDVRRRLALVLDTDDMVEAHRLAVQMRPWFGVAKV
                     GLELFSAAGPDVIADLRDLGYQVFLDLKLFDIPTTVNRATRVLGALGVEYLTLHATGG
                     VDMLKAGVEGLYHGAEAAGLDVPVSLAVTVLTSDDSAPPHIVPNRVRVALEGGCGGLI
                     LAAGDLQTARELAPRLRRVVPGIRPAGSPIHDQARAATPQEAIANGADLLVIGRAVTQ
                     ADDPVAAAAAIASAIG"
     gene            complement(2056..2982)
                     /locus_tag="PKY13_13895"
     CDS             complement(2056..2982)
                     /locus_tag="PKY13_13895"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOT81081.1"
                     /translation="MSVDLSVSVGSITLPNPVMVASGTAGHSTELSAYLDLAGLGAFV
                     VKSLSADPWDGNPGPRIHQTPAGMINSVGLQGPGVAAWLGDGLARLRRIGVERIVVSI
                     WGRTVDEYRRAARMLAEAPASVIGVEVNLSCPNLDGGRHLFAHDPDTAAEVIEACGVA
                     DRPLWAKLSPNTDRTAEVAGAVAAAGAEAVTLTNTLLGMSIDPETRRPRLGSGAGGGG
                     VSGPAIHPVAVRAVYDCRVAHPDLPIIGVGGVATAADAVEFLLAGAQACQVGTATFAD
                     PRAPRHVLDDLRAWCEAHKIDRVRELSGGAHG"
     gene            complement(3086..>4607)
                     /locus_tag="PKY13_13900"
     CDS             complement(3086..>4607)
                     /locus_tag="PKY13_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT81082.1"
                     /translation="ETVMVNCNPETVSTDYDTSDRLYFEPLTFEDVMSVIEAEQAAGP
                     VAGVIVSLGGQTPLKLADVLPRELILGTSPDSIDLAEDRDRWNALCARLEIPQPAGGT
                     ATDLAGAQAITDRIGYPALVRPSYVLGGRAMEIVYDDEGLATAMATLAGLGGLGREGG
                     LSASRPVLIDRFLEDATEVDVDAIRDSTGEVLIGGIMEHVEEAGVHSGDSACVLPPMT
                     LGAGTLAVIEDYTKRLAESLEVDGLINVQFAVKRSGNTPDAAQVFVIEANPRASRTVP
                     FVAKATGVPLVKVASRVMCGASLAQLRLEGLLQPPVTGDHISVKEAVLPFNRFPDADA
                     VLGPEMRSTGEVMGIDLTFGLAFAKSQLAAGDRMPTTGCVFMSLADRDKETGLRAAQR
                     FADLGFEIVATAGTAAFFTEAGVVVSQVVDKLGEGGSDAVQLLSSDRIQLVVNSPRGR
                     GPRSDGAYIRSAAGLHRVPLLTTAAAALAAANGIADTAAHEPVVRSLQEYHAGVHK"
BASE COUNT          710 a         1628 c         1615 g          654 t
ORIGIN      
        1 gctcggccgg cgtcggcggc cttctgccac agattgcggg cagcctcgaa gtagacccag
       61 gtgtacttga agtcgtcgtc ggacagatca gcccacgcgt acgtctcggg gtcgatgagg
      121 tcgtactgcg cccgcagtgc gtcgctgtca atggcggcga gcgcagcagc ggtcgccgcc
      181 acctcgtcgg cgggggtgaa ggccacggtg cggtcgtcct cggtggtgag cgtcgcgctc
      241 ccgaagatgg cccgactctc gggcgcctcg gactcgtcga gcgccgcgct cccggcgacg
      301 cacatgtgga tcgcgtacca ggccttgtcg gtctcggcga cgaagtcctt ctcccaccgc
      361 tcctggatcg tgcccttgac gtacttcacg acctcggcgt gaccgtcggc acccagcagc
      421 gcggcgacat ctccgtcgcc gattgcgaac aacactccac gtccggccat ctgtggtcct
      481 ctcagtcggg ttcggaactg gcccgagcag cacgacgccg ctcgtcgacc agcgccgcga
      541 ggtggtcgac cgtatcggac acctcgtggt tcaccaccag caccgagtcg agatccttcg
      601 ctgcggcagc ctcttcgtcg gctcgggcga cgcgctgctc gacccgttcg ggcgtatcac
      661 cacggtggac gagccgctcg cgctgcacgt cacgggacgg cgcttcgagg tagatgcaca
      721 gagcgtcggg gaatttctcc ttgaccgcct tggccccgta gacgtcgatc tcgaagatga
      781 cgtcgtggcc gtcgggcggt tcgggcacgg gactcccctg ccggtagtcg aggaaatcga
      841 cccactcgag gaacccgccg tcgtcgatgc gtcgctggaa ctcgtcccac gtggcgaaca
      901 cgtaggcgtc ctcggcttcg ccggggcgac gctcacgggt cgtccacgac cggttgacgt
      961 acagcttggg atcgcgctca cgccacgcgg cgacgaccgt gcccttgccg gcaccgccgg
     1021 gaccggacac cacgatgatc atgcccgctc ccccaacagg accgaccagg cggcctcggg
     1081 gatggcgccg agctcgatgt cctcggggac gccggcagcc gccatgagcc ggcgcgacgc
     1141 gaccttgccg ccgacggcgg ggatcgcctc gagcacgggc aggagacgca gctcggccag
     1201 gcgatccacc tcccgcgccg ggaggacgtg gcgttcacgt tcgagttcgg cgcgcacgtc
     1261 cgcgagcgag aggccggcgg aacaacgccc gacgaaacgc tcccggaagg cacgggtgcg
     1321 agccgcccac tcgagggcgt cgtggagcac gacggtggtc acccgatcgc ggaggcgatg
     1381 gccgcagcgg cggcgacggg atcgtcggcc tgcgtcacgg cccggccgat caccaggagg
     1441 tcggcgccgt tcgcgatggc ctcctggggc gtggcggccc gggcctggtc gtggatcggc
     1501 gatccggccg gccggatgcc ggggacgacc cgccggagac gcggcgccag ttcccgtgcg
     1561 gtctgcagat cgccggcggc gagaatcaga ccgccgcagc caccctcgag cgccactcga
     1621 acccggttcg gaacgatgtg gggcggcgcg gaatcgtcgc tcgtcagcac ggtgacggcc
     1681 aacgagacgg gcacgtcgag cccggcggcc tcggcgccgt ggtacaggcc ctcgacgccg
     1741 gccttgagca tgtcgacgcc accggtggcg tgcagcgtga ggtattccac cccgagcgcg
     1801 ccgagcacgc gggtggcgcg gttcaccgtc gtcgggatgt cgaagagctt caggtccaag
     1861 aagacctggt agccgaggtc acggagatcg gcgatcacgt cggggccggc ggcgctgaac
     1921 agctcgaggc cgaccttcgc cacgccgaac cagggccgca tctgcaccgc gagccggtgg
     1981 gcctcgacca tgtcgtcggt gtcgagcacc aacgccagtc tccgacggac gtcctccgaa
     2041 acctcgcctg caatgtcagc catgtgcgcc tccgctgagt tctcgtactc gatcgatctt
     2101 gtgggcctcg caccaggccc gcaggtcatc cagcacgtgc cggggagctc ggggatctgc
     2161 gaatgtggcc gttcctacct gacacgcctg agcaccggcg agcaggaact cgacggcatc
     2221 ggccgcggtc gcgactccac ccaccccgat gatggggagg tcggggtgag cgacccggca
     2281 atcgtagaca gctcgcaccg ctaccggatg gatggccgga ccggagacgc cccctccccc
     2341 ggcgccgctg ccgaggcgcg gccgacgggt ctcgggatcg atcgacatgc ccagcagcgt
     2401 attggtgagg gtgacggcct cggcgccggc agcggcgacc gccccggcga cctcagcggt
     2461 gcggtcggtg ttggggctga gcttggccca gagcggccgg tcggcgacac cacacgcctc
     2521 gatcacctcg gccgcggtgt cgggatcgtg ggcgaacagg tgccggccgc cgtcgaggtt
     2581 cggacagctg aggttgacct ccaccccgat caccgaggcc ggcgcctcgg cgagcattcg
     2641 ggcggcgcgg cggtactcgt cgacggtccg gccccagatg gagaccacga tgcgctcgac
     2701 gccgatgcgg cgcagcctgg cgagaccgtc gccgagccag gcggcgacac cgggcccctg
     2761 gaggccgacg ctgttgatca tccccgccgg cgtctgatgg atgcgggggc cggggttgcc
     2821 gtcccacggg tcggcggaga gggacttcac gacgaaggcg ccgagtcccg cgaggtcgag
     2881 gtacgccgac agctccgtcg agtgaccggc cgtgcccgac gcgaccatca ccgggttggg
     2941 cagggtgatc gacccgaccg agaccgacag atcgacgctc atgacccggg cgagtgccga
     3001 aggcggctcg gcccatgagg acagtttcgt tggtcggcga agccgacggc ctctggagtc
     3061 accgacgtga gcggactcca ccgactcact tgtgcactcc ggcgtggtac tcctgcaggc
     3121 tccgcacgac cggctcgtgc gccgcggtgt ccgcgatgcc gttggcggcc gcgagtgctg
     3181 cagcggccgt cgtcaggagc ggcacccggt gcaacccggc ggcggagcga atgtacgcac
     3241 cgtcggaacg gggcccacga ccacgcggcg agttcaccac gagctggatc cggtcgctcg
     3301 acagcagttg cacggcgtcg ctcccgccct cgccgagctt gtcgacgacc tggctcacca
     3361 cgacgcccgc ctcggtgaag aacgctgcgg tgccggcggt cgcgacgatc tcgaaaccga
     3421 ggtcggcgaa ccgctgtgcc gcccgcaggc cggtctcctt gtcgcgatcg gccaacgaca
     3481 tgaacacaca ccccgttgtc ggcatccggt cgcccgccgc gagctgcgac ttggcgaacg
     3541 ccagcccgaa cgtcaggtcg atgcccatga cctcgccggt cgagcgcatc tcgggaccga
     3601 gcaccgcatc ggcgtcgggg aaccggttga acggcagcac cgcctccttg accgagatgt
     3661 ggtcgccggt gaccggcggc tgcaacagcc cctcgagccg aagctgcgcg agcgatgcgc
     3721 cacacatcac ccgcgacgcc accttcacga gcgggacccc ggtcgccttg gccacgaagg
     3781 ggacggtgcg cgacgcccgg ggattcgcct cgatgacgaa gacctgggcg gcgtcgggag
     3841 tgttgcccga ccgcttcacc gcgaactgca cgttgatgag accgtcgacc tcgagcgact
     3901 ccgcgagccg cttggtgtag tcctcgatga cggcgagggt gcccgcaccc aacgtcatcg
     3961 gcggcaagac acacgccgag tcaccggagt gcacgcccgc ctcctcgacg tgctccatga
     4021 tcccgccgat caacacctca ccggtcgaat cgcggatggc gtcgacgtcc acctcggtgg
     4081 cgtcctcgag gaaccggtcg atcagcaccg gccgggaggc ggacaggccg ccctcacgcc
     4141 cgagtccgcc gagtccggcg agggtggcca tcgccgtggc aaggccctcg tcgtcgtaca
     4201 cgatctccat ggcccgtcca ccgagcacgt acgacggccg gacgagggcc gggtacccga
     4261 tgcggtcggt gatcgcctgg gcgccggcca ggtcggtggc cgtaccaccg gcgggctggg
     4321 ggatctcgag ccgcgcacac agcgcattcc accggtcgcg gtcctcggcg aggtcgatcg
     4381 agtcggggct ggtgcccaag atcagctcac ggggcagcac gtcggcgagc ttgagcgggg
     4441 tctggccgcc gaggctgacg atcacgcccg ccaccggacc cgccgcctgc tcggcctcga
     4501 tgaccgacat cacgtcctcg aacgtcaacg gctcgaagta gaggcggtcc gacgtgtcgt
     4561 agtcggtcga gacggtctcg gggttgcagt tgaccatcac ggtctcg
//
LOCUS       DAOSOA010000274         4573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_18960_length_4573_cov_6.342187, whole genome shotgun sequence.
ACCESSION   DAOSOA010000274 DAOSOA010000000
VERSION     DAOSOA010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18960_length_4573_cov_6.342187"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKY13_13905"
     CDS             <1..972
                     /locus_tag="PKY13_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HOT81083.1"
                     /translation="GMSQRLEANSRDRAALVFPFTHIGGITWLFSALQTGCSLILMES
                     FHPTETPEVLSRESVTLAGSGTPFHMAYLAAQRASLHPIFPDVRAFPGGGAAKPPQLA
                     HDLRAVFDAPVLSGYGLTEAPILTMAATTDSDEELALTEGKAMPGVDLKLVRPDGSSA
                     AEGEDGEVRAKAPQLMKGYLDASLDADAFDEDGYFRTGDLGRFDTKGNLIITGRLKDV
                     IIRKGENISAKELEDLLFQHPKVGDVAVVGLPDPMSGERACAVVQTATGTEPIGFDEM
                     VAFLKDAGLMVQKLPEQLEILNTIPRNASGKVLKHVLREQFGDLGRP"
     gene            969..1475
                     /locus_tag="PKY13_13910"
     CDS             969..1475
                     /locus_tag="PKY13_13910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT81084.1"
                     /translation="MTDPSGAAIRAAAPDDLEQIVELNNEAVPAVSVADVERMAELVD
                     LAAAAWVVDDGEGGLAGFVLLFEPGSVYDSANYRWFAERFDAFLYVDRVVVAAGQRGG
                     GLGATLYEAVAAEAGRRGSGRVLAEVNLEPPNPGSSRFHRRHGFEAIGELRFSDDYVV
                     EMLERPVP"
     assembly_gap    1523..1622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1700..1882)
                     /locus_tag="PKY13_13915"
     CDS             complement(1700..1882)
                     /locus_tag="PKY13_13915"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOT81085.1"
                     /translation="MLTSYEFIAAYLQAKVRSERGASLVEYALLVALIAVVCIAAVTA
                     LGKKASSNFNSIATSI"
     gene            2205..3119
                     /locus_tag="PKY13_13920"
     CDS             2205..3119
                     /locus_tag="PKY13_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT81086.1"
                     /translation="MGLLDGKVAIVTGAGHGIGRGHALELARHGAKVIVNDLGVSVTG
                     EGGGRDADLTVDIIKERGGEAAGNYENVADFDGAGRMVQQAIDEFGRLDILVNNAGIV
                     RDSTIVKMSEADFDAVFNVHVKGTWAPAKHAATYWTDKFKETGVPVGGRIINTTSGAG
                     LTGNFGQTNYASAKAAIASMTQTLSLELYKAGVTVNCVGPAAATRITGTMPGAPKVIE
                     ADDVPLDEWNRMDPAVSSPLVAWLASDEADHVTGQVIRAVAENIILMKPWSDGPTINN
                     GQKRWDATKLGTQLATDVFFTRAPGLRY"
     gene            3140..4459
                     /locus_tag="PKY13_13925"
     CDS             3140..4459
                     /locus_tag="PKY13_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81087.1"
                     /translation="MPSSPGEPDGHVTGPVTNGDVIADALGELGVRRVYGSPLGDLPV
                     VDPGDPDLTFLLADTDGRMGNGWGAAFVDGQILHLSSQPGGTARPRTITGIDGLVAAL
                     VEAELDAVPRTLAFELDLDLDAPVDEDLDLSGRPDGGVLVTLSPSLADLAIVAVVGPG
                     VGRGETEALAEFAARGGIGVYNTWGAKGVFRWDSPFHYGTIGLQAHDLDLAELGSFDL
                     VIASGLDPDEFGIDALAGFLVQEVAPWQIGALLADWPRAVRSVDDRPPLYDTIASVIT
                     PMYEQESGPVTPPRAALQLSGAAPDHAVIAADPGTAGFWIARTFPTGVPGSVVVPALR
                     QPGFAVAAGIVAGLDGRPCVSVVDGPLDDASEAALEVARRLGVSVAVQVWEPGDTSAD
                     DHLHLTRAGFAAAASGSERIDTVGVDATCLEVLTDAVGPVTAWQHGS"
BASE COUNT          686 a         1520 c         1532 g          735 t
ORIGIN      
        1 ggcatgtccc aacgtctcga ggcgaacagc cgggaccgtg ccgcgctcgt gttcccgttc
       61 acccacatcg gtggcatcac ctggttgttc agcgccctgc agacggggtg ctcgctcatc
      121 ctcatggagt cgttccaccc gaccgagacc cccgaggtcc tgtcgaggga gagcgtgacg
      181 ctggccggct cgggaacgcc cttccacatg gcgtacctcg ctgcgcagcg ggcgtcgctc
      241 cacccgatct tccctgacgt ccgggccttc cccggcggtg gtgcagcgaa gccgccgcag
      301 ctcgcccacg atcttcgagc cgtcttcgac gcccccgtcc tgtcgggcta cgggctcacc
      361 gaggcaccga tcctcacgat ggcggccacc accgactccg acgaggagct ggccctgacc
      421 gagggcaagg cgatgccggg ggtcgacctg aagctggtgc gtcccgacgg ctcgtcggcg
      481 gccgaaggtg aggacggaga ggtgcgggcc aaggcgcctc agctcatgaa gggctacctc
      541 gacgcgtcgc tcgacgccga cgcgttcgac gaggacggct acttccgtac cggcgatctc
      601 ggccggttcg acaccaaggg caacctcatc atcacgggcc gcctcaagga cgtgatcatc
      661 cgcaagggcg agaacatctc ggcgaaggag ctggaggacc tgttgttcca gcaccccaag
      721 gtgggcgatg tcgcagtggt ggggctcccc gacccgatgt cgggcgagcg ggcgtgcgcc
      781 gttgtccaga ccgctaccgg caccgaaccg atcgggttcg acgagatggt tgcgttcctg
      841 aaggacgccg ggctcatggt gcagaagctg cccgagcagc tcgagatcct caacacgatc
      901 ccgcgcaacg cgtcgggcaa ggtgctcaag cacgtgctgc gtgagcagtt cggcgacctg
      961 ggtcgcccgt gacggacccg agcggagcgg ccatccgggc ggcggcgcct gacgacctgg
     1021 agcagatcgt cgagctcaac aacgaggcgg tcccggcagt gtcggtcgcc gacgtggagc
     1081 ggatggcgga actggtcgac ctcgccgccg ctgcgtgggt ggtcgacgat ggcgagggtg
     1141 gactcgccgg gttcgtgctg ctgttcgaac cggggtcggt ctacgacagc gccaactacc
     1201 ggtggttcgc cgagcggttc gacgcgttcc tgtacgtcga ccgcgtcgtg gtcgctgccg
     1261 gtcagcgcgg cggcggactg ggggcgacct tgtacgaagc ggtcgcggcg gaggccggcc
     1321 gtcgtggatc gggtcgggtg ttggccgagg tcaacctcga acccccgaac ccgggctcgt
     1381 cgcgattcca ccgacggcac gggttcgagg cgatcgggga actccggttc tccgacgact
     1441 acgtcgtgga gatgctcgag cgaccggtgc cctgaccgac ggtcgtcaga tcgacgtggc
     1501 gatcgagttg aagttggact tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nncctcatgg gcccgaatac cgggacggga ccggtcgtcg accggtcccg ccccagggag
     1681 tattcggatg tccgaacgat cagatcgacg tggcgatcga gttgaagttg gacgacgcct
     1741 tcttgccgag ggccgtgacg gctgcgatgc agaccacggc gatgagggcc acgagcaggg
     1801 cgtactccac gagggaggcg ccacgctcgg agcggacctt ggcctgaagg taggcggcga
     1861 tgaattcgta gctggtgagc attggagtga tccttttggt gtttggtgct tcctggtgtg
     1921 gcgttgggcg gtcactctcc caggttgggt aagcgctctc cgtggccgaa tctctttgta
     1981 cgagcaacgc catcggttcc caatagtccg ttcggacttt cggttacccg cactgacgag
     2041 acccatgtga ggtatcgggg gagagcgatg aggacttgag cgttttccgc cggttctcgg
     2101 ggcacgctcg ttggtccgcc cccggcccga ccacctacta tctgacggtt cgtcagaaac
     2161 ggtccggtca cgagaacgga ccaacgctcc aggaggggtc aaccatgggc ttgctcgatg
     2221 gcaaggtcgc aatcgtcacg ggtgccggtc acggcatcgg ccggggtcac gcgctcgagc
     2281 tcgcccgtca cggcgccaag gtgatcgtca acgatctcgg cgtctcggtc accggtgagg
     2341 gcggaggtcg agatgccgac ctcaccgtcg acatcatcaa ggagcgcggc ggcgaggccg
     2401 ccggcaacta cgagaacgtc gccgacttcg acggcgccgg ccggatggtg cagcaggcga
     2461 tcgacgagtt cggccggctc gacatcttgg tcaacaacgc cggcatcgtg cgggactcca
     2521 ccatcgtgaa gatgtccgaa gccgacttcg acgccgtgtt caacgtgcac gtcaagggca
     2581 cgtgggcgcc cgccaagcac gcggcgacct actggaccga caagttcaag gagacgggtg
     2641 tccccgtcgg cggtcgcatc atcaacacca cgtcgggcgc cggcctcacc ggcaacttcg
     2701 gtcagaccaa ctacgcctcc gccaaggcgg ccatcgcgtc gatgacccag accctcagcc
     2761 tcgagctgta caaggccggc gtgacggtca actgtgtcgg ccccgccgcc gccacccgca
     2821 tcaccggcac gatgcccggc gcgccgaagg tcatcgaggc cgacgacgtg cccctcgacg
     2881 agtggaaccg catggatccc gccgtcagct cgccgctcgt cgcctggctc gcatccgacg
     2941 aggccgatca cgtgaccggc caggtcatcc gcgccgtcgc cgagaacatc atcctgatga
     3001 agccgtggtc cgacggtccg acgatcaaca acggccagaa gcgctgggat gcaacgaagc
     3061 tcggcacgca gctggccacc gacgtgttct tcacccgggc tccgggcctc cgctactagg
     3121 cggagcgtca ccttctccca tgccgagctc cccgggtgaa cccgacggtc acgtcacagg
     3181 accggtcacc aacggcgatg tgatcgccga cgcgctgggt gagctcggcg tgcggcgggt
     3241 ctacggcagt ccactcggcg accttccggt cgtcgatcct ggcgaccccg acctcacctt
     3301 cctgttggcg gacaccgacg gccggatggg gaacggctgg ggcgcggcct tcgtggacgg
     3361 ccagatcctg cacctgtcct cccagcccgg ggggacagct cggccgcgga cgatcacggg
     3421 aatcgacggc ctcgtcgccg cgctcgtcga ggcggagctc gatgccgttc cgcggactct
     3481 cgccttcgaa ctcgacctcg atctcgatgc cccggtcgac gaggacctcg acctgtcggg
     3541 ccgccccgac ggaggggtgc tcgtcaccct gtcgccgagc ctcgccgacc tcgccatcgt
     3601 ggcggtcgtc ggaccgggtg tcggtcgcgg cgagaccgag gcgctggccg agttcgcggc
     3661 acgcggcggc atcggcgtct acaacacctg gggtgccaag ggagtgttcc gttgggacag
     3721 ccccttccac tacggcacga tcgggttgca ggctcacgat ctcgacctgg cggagctcgg
     3781 gtcgttcgat ctcgtcatcg cgtccgggct cgatcccgac gagttcggca tcgatgccct
     3841 cgccgggttc ctcgtgcagg aggtggcgcc gtggcagatc ggcgcgctcc tcgccgactg
     3901 gccccgggcg gtccgttccg tcgacgaccg cccgccgctc tacgacacga tcgcgtccgt
     3961 catcaccccg atgtacgaac aggaatcggg gccggtgacc ccgccccgtg ccgccctcca
     4021 gctgtcgggc gctgcgcccg atcatgccgt gatcgctgcg gatcccggta ctgcggggtt
     4081 ctggatcgcc cgtacgttcc ccaccggcgt gcccggatcg gtggtcgtcc cggcgcttcg
     4141 tcaaccgggg ttcgccgttg ccgccggtat cgtcgccggt ctggacggtc gaccgtgtgt
     4201 gtcggtcgtc gacggcccgc tcgacgatgc ctccgaggcg gccctcgagg tcgctcgtcg
     4261 actcggcgtc tcggtcgccg tgcaggtgtg ggagccgggc gacacgagcg ccgacgatca
     4321 cctccacctc acccgcgccg gcttcgccgc cgctgccagc ggttcggagc ggatcgacac
     4381 ggtcggggtc gacgcgacgt gcctcgaggt cctcaccgac gccgtcggtc ccgtgaccgc
     4441 ctggcagcac ggctcctgac caccaccgat ctcgtgggag cgcacccgcc gggtgtcggg
     4501 gattcctaga acacgtttca gtttcgtgcc acgatagggg gtcggtcggc gggttcggcc
     4561 gaggaggaac ggt
//
LOCUS       DAOSOA010000275         4567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_18993_length_4567_cov_7.529699, whole genome shotgun sequence.
ACCESSION   DAOSOA010000275 DAOSOA010000000
VERSION     DAOSOA010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18993_length_4567_cov_7.529699"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKY13_13930"
     CDS             <1..501
                     /locus_tag="PKY13_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81088.1"
                     /translation="YHDYWSYWWQLPMAMCFAGGIEAIARSTTTQRRNVAAVAAVVLV
                     AAGPLWTLTGRSQAEREYRRGLEVAALLESIERPPDQDHVLTFGLGLPVSWASWSTDE
                     PVDTLNLAQLREIATIHPDWMVIAHCEVKLPDRGPGLCDRLPAWAPRAGDVVVADPVQ
                     LAAVIG"
     gene            563..2206
                     /locus_tag="PKY13_13935"
     CDS             563..2206
                     /locus_tag="PKY13_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOT81089.1"
                     /translation="MAYNIADLFEHVVDAVPDRLALVDRDTRLTFAELDERANRIAHH
                     LGAQGIGVGDHVGIYAQNCHEWIETMLGCFKLRAIPINVNFRYVSDELAYIFNNADLV
                     GLVFDDQYAPLVTEIRDKAPRLKAFVHVHTGIGDDGTDVSADLAALGSVSYEDATVDQ
                     SGSRDGLPGDRSPDDLYVLYTGGTTGMPKGVMWRQEDVYFALGQGIDAVTGERVTSEF
                     TMAEKAAASGGGLVFMVIPPMMHGAAQWGTMGQMFQGNTIVLLPKFSGEAVWELVEKE
                     RVNAALITGDAMGRPMIEHLEANSDKYDTSSLIAVSSSAAIFSPTVKDHFLEYFPNLI
                     ITDSIGSSESGFNGIRTVAKGQAQNENGGPTVTPGPDMVILDEQLRIMPKTSREQGKM
                     GRGGNIPLGYFNDEKKTAETFVVADDGKRYAVSGDWARWEDDGLVTLLGRGSTTINSG
                     GEKIHPEEVEQALKDHPAVFDCIVVGVPDERWGQRVAAVVQFREDMDAELEHLADHAR
                     KFVAGYKVPRELHVVPEIVRSPSGKPDYPWASKLARGEA"
     gene            2206..2976
                     /locus_tag="PKY13_13940"
     CDS             2206..2976
                     /locus_tag="PKY13_13940"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOT81090.1"
                     /translation="MAGLTPEADGSYLVEDQRVTMPVEVRAAHQAAATFLVRHAAAQQ
                     VIGHTGLTATRQRGGFAIASLALVDYIDNDLGSYKELALAFVVDDIGAPADANPKDGK
                     QVSTLIHRLPVSESFTCAAGRGIWGFPKWIADLSVDFTPKGATCVLRADGQDVVTVTM
                     RRGPIPVPRRPMAMNAYSCDDDGIVRRTPWTTHSLGRQTVRPGGTTVEIGYGHPLADE
                     LRTLGLPKRSLATLFDDHMAATFGAPQVVDTGTHKITV"
     gene            2973..3941
                     /locus_tag="PKY13_13945"
     CDS             2973..3941
                     /locus_tag="PKY13_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81091.1"
                     /translation="MTARRSSARPRTLTRAADIARRFGAAAAAACLVAIPALAIAPTV
                     AAQAPAGAPDRLAAAQQSIGVVRIGERERSSTGWIAADGLVVTSDIIASEPGEAVTFT
                     TGSAEPVACYTAVVERALHLAYLRCAELEGVALRVATGYPAPGVGVTVALATPAGDAF
                     GVTTEAGAVTANDLAFMGANRLQFSMWVNRSEQPAARPGILDSENSTGAPVLDAEGRV
                     ISTILTAPEAGGQPIGTTPSELAKGVKKAAPLPATISSAAVLTVARRALIPAAVGLVL
                     GLVWAMITRNGNILAKTLGLTALGLVGAIAYTLFTLLVVGPQTLIG"
     gene            complement(3962..>4567)
                     /locus_tag="PKY13_13950"
     CDS             complement(3962..>4567)
                     /locus_tag="PKY13_13950"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOT81092.1"
                     /translation="GITGVGQVVDAAMVDGAALLMAPLFGAYDSGYWSDERGTNLLDS
                     GAPFYDCYQCADGGYVAVGALEPQFFAALVAGLDLDPTTLPSQNDRDRWPELREAFET
                     RFRARSRDEWAEHFSTMDACVSPVLTMGEAPSYPHNVERDGYVQVGGVRQPGPAPRFS
                     ESPAPVPSPPQIAGANTDEVLARWGIDADERTRLRSAGAIG"
BASE COUNT          738 a         1668 c         1511 g          650 t
ORIGIN      
        1 taccacgact actggtcgta ctggtggcaa ctcccgatgg cgatgtgctt cgccgggggg
       61 atcgaggcga tcgcgcggtc gacgacaacg caacggcgca acgtcgccgc ggtcgctgcc
      121 gtggtcctcg tcgccgccgg accgctctgg accctcaccg gtcgcagtca ggccgaacgg
      181 gagtaccgcc gcggactcga ggtggcggcg ctgctcgaat cgatcgagcg cccgccggac
      241 caggaccacg tcctgacctt cggactcggg ctccccgtct cgtgggcatc gtggtccacc
      301 gacgaaccgg tggacacgct caacctcgca cagctgcgcg agatcgcgac aatccatccc
      361 gactggatgg tgattgccca ttgcgaggtc aagctgcccg atcgagggcc cggattgtgc
      421 gatcggctgc ccgcctgggc accccgcgcc ggcgacgtcg tcgtggccga tccggtgcaa
      481 ctggcggccg tgatcggctg accgccaccg atccaccgtg atcaacggga accggaacga
      541 gaacatgttc tagattcgga gcatggccta caacatcgct gatctgttcg agcacgtcgt
      601 cgacgccgtc cccgaccgtc tggccctcgt cgaccgcgat actcgcctca ccttcgcgga
      661 gctcgacgag cgagcgaacc gcatcgccca ccacctcggg gcccagggca tcggcgtggg
      721 cgaccacgtc ggcatctacg cccagaactg ccacgagtgg atcgagacga tgctcggctg
      781 cttcaagctg cgggcgatcc cgatcaacgt caacttccgg tacgtgtccg acgaactggc
      841 gtacatcttc aacaacgccg acctcgtcgg cctcgtgttc gacgaccagt acgccccgct
      901 cgtcaccgaa atccgcgaca aggcgccgcg tctcaaggcg ttcgtgcacg tccacacggg
      961 catcggtgac gacggcaccg atgtgagcgc cgacctcgct gcgctcggat ccgtgtccta
     1021 cgaggacgcc acggtcgatc agtccggcag ccgggacggc ctgcccggcg accgctcccc
     1081 cgacgacctg tacgtgctct acaccggcgg aacgactggc atgcccaagg gcgtgatgtg
     1141 gcgccaggag gacgtgtact tcgcgctcgg gcagggcatc gacgccgtca ccggcgagcg
     1201 ggtcacgagc gagttcacga tggccgaaaa ggctgccgcg tcgggtggcg gcctcgtctt
     1261 catggtgatc ccgcccatga tgcacggcgc tgcccagtgg ggaaccatgg gtcagatgtt
     1321 ccagggcaac acgatcgtgc tgctccccaa gttcagcggc gaggcggtgt gggaactcgt
     1381 cgagaaagag cgggtcaacg ccgcgctcat caccggtgac gccatgggtc gcccgatgat
     1441 cgagcacctc gaggccaaca gcgacaagta cgacacgtcg tcgctgatcg ccgtgagctc
     1501 ctcggcagcc atcttcagcc ccacggtgaa ggaccacttc ctcgagtact tcccgaacct
     1561 gatcatcacc gactcgatcg gttcgtcgga gtccgggttc aacggcatcc gcaccgtcgc
     1621 caagggccag gcccagaacg agaacggtgg ccccacggtc acgcccggcc ccgacatggt
     1681 catcctcgac gagcagctcc gcatcatgcc gaagacgagc cgcgagcagg gcaagatggg
     1741 ccgcggcggc aacatcccgc tcggctactt caacgacgag aagaagacgg ccgagacgtt
     1801 cgtcgtcgcc gacgacggca agcgctacgc cgtgtcgggc gactgggccc ggtgggagga
     1861 cgacggcctc gtcaccctgc tcggacgcgg ctccaccacg atcaactcgg gcggcgagaa
     1921 gatccacccc gaagaggtcg agcaggcgct caaggaccac cctgccgtct tcgactgcat
     1981 cgtcgtcggc gttcccgacg agcgatgggg ccagcgggtc gccgccgtcg tgcagttccg
     2041 cgaggacatg gatgccgagc tcgagcacct cgccgaccac gcccgcaagt tcgtcgccgg
     2101 ctacaaggtt ccccgcgagc tccacgtggt gcccgagatc gtgcggtcgc cgagcggcaa
     2161 gcccgactac ccctgggcgt cgaagctcgc acggggcgag gcctgatggc cggcctcacg
     2221 ccggaggccg acggctccta cctcgtcgag gaccagcggg tcacgatgcc ggtcgaggtg
     2281 cgtgcggccc accaggccgc agcgacgttc ctcgttcgcc acgccgccgc ccaacaggtg
     2341 atcggacaca cggggctcac cgccacgcgc cagcgcggcg ggttcgcgat cgcctcgctc
     2401 gcgctggtcg actacatcga caacgacctc ggcagctaca aggagttggc gctggcgttc
     2461 gtcgtcgacg acatcggtgc tcccgccgac gccaacccga aggacggcaa gcaggtcagc
     2521 accctcatcc accggttgcc ggtgagcgag tcgttcacgt gcgccgccgg tcgcgggatc
     2581 tggggcttcc ccaagtggat cgccgacctg tcggtcgact tcacgccgaa gggcgccacc
     2641 tgcgtgctgc gcgccgacgg ccaggacgtc gtcaccgtca ccatgcgccg cggcccgatc
     2701 ccggtcccgc gccgcccgat ggcgatgaac gcctacagct gcgacgacga cggcatcgtt
     2761 cgtcgaacgc cgtggaccac ccacagcctc gggcgccaga ccgtgcgccc cggcggtacg
     2821 accgtcgaga tcggctacgg ccatccgctg gccgacgagc tgcgcaccct cgggctgccc
     2881 aagcggtcgc tcgccacgct cttcgacgac cacatggcgg ccacgttcgg tgccccccag
     2941 gtcgtcgaca cgggcacgca taagatcacg gtgtgaccgc ccgccgctcg agcgcccgtc
     3001 cccggaccct gacgagagcg gccgacatcg cccgccggtt cggtgctgcc gccgctgcgg
     3061 cgtgcctcgt cgccatcccg gccctcgcca tcgcgccgac ggtcgcagca caagcgccgg
     3121 ccggagcgcc cgatcggctc gccgccgccc agcagtccat cggcgtggtc cgcatcggcg
     3181 agcgcgaacg gtcgagcacc ggctggatcg ccgccgacgg cctcgtcgtg acgtcggaca
     3241 tcatcgcgtc cgagccgggg gaggccgtca ccttcacgac gggttcggcc gaaccggtcg
     3301 cgtgctacac cgccgtcgtc gagcgggcac tccacctcgc atatctgcgc tgcgccgagc
     3361 tcgagggcgt cgcactccgc gtcgcgacgg gctatcccgc gccgggcgtc ggggtcaccg
     3421 tcgctctcgc aacgccggcc ggcgatgcct tcggcgtgac gaccgaggcc ggagcagtga
     3481 cggcgaacga cctcgccttc atgggcgcca accgcctcca gttctcgatg tgggtgaacc
     3541 ggtcggagca gcccgcagcg cgacccggca tcctcgacag cgagaactcg accggggcac
     3601 ccgtcctcga cgccgagggg cgggtgatca gcacgatcct gaccgccccc gaggccggcg
     3661 gacagccgat cggcacgacc ccgtcggagc tcgccaaagg cgtgaagaag gcggcgccac
     3721 tcccggcgac gatcagttcg gcggcagtgc tcacggtggc ccgccgggcg ctcattcccg
     3781 ccgcggtcgg cctggtgctg gggctcgtct gggcgatgat cacccgcaac ggcaacatcc
     3841 tcgccaagac cctgggcctg actgccctcg gcctggtggg agcgatcgcc tacaccctgt
     3901 tcaccctgct cgtggtggga ccgcagacgc tcatcgggtg agctgatccg atcggcagcc
     3961 gtcagccgat cgcaccggcc gaccgcagcc gggtgcgttc gtcggcatcg atcccccacc
     4021 gagcgagcac ctcgtcggtg ttggccccgg cgatctgcgg gggcgacggc actggggccg
     4081 gggattcgct gaagcgcggc gccggccccg gctgccgcac cccaccaacc tgcacgtagc
     4141 cgtcgcgttc gacgttgtgg gggtacgacg gggcctcgcc catcgtgagt accggggaca
     4201 cacaggcgtc catcgtcgag aagtgctcag cccactcgtc tcgggaacgg gcccggaagc
     4261 gagtctcgaa cgcctcccgg agttcgggcc accggtcgcg gtcgttctgg gacggcagcg
     4321 tggtcgggtc gaggtcgagg ccggccacca acgccgcgaa gaactgcggc tcgagcgccc
     4381 ccaccgcgac gtacccgcca tcggcacact gatagcagtc gtagaacgga gcgccggagt
     4441 cgagcaggtt ggtcccccgt tcgtcgctcc agtacccgga gtcgtaggcg ccgaacagcg
     4501 gcgccatgag cagcgcggcg ccgtcgacca tcgcggcgtc gacgacctgg ccgacgccgg
     4561 tgatgcc
//
LOCUS       DAOSOA010000276         4566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19000_length_4566_cov_5.378852, whole genome shotgun sequence.
ACCESSION   DAOSOA010000276 DAOSOA010000000
VERSION     DAOSOA010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19000_length_4566_cov_5.378852"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKY13_13955"
     CDS             <1..504
                     /locus_tag="PKY13_13955"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT81093.1"
                     /translation="VLEQQTQKRIIVFRPLFAVGDQQLGYLPGSESEKMSPWSAAVMD
                     ALESIASPQVIDLIVADGLLEVLPLTHIRGRTLVDTFVILDEAQQYERPVLVTALSRL
                     GAGSRVVLTHDVAQRDNLRVGRYDGVMSVIGALTGNPLFGHVTLSRSERSPIAALAAS
                     ILDVPVP"
     gene            641..1405
                     /locus_tag="PKY13_13960"
     CDS             641..1405
                     /locus_tag="PKY13_13960"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HOT81094.1"
                     /translation="MRTRFNGLGRVLVGTTAVGAGLLLAAVPASAHVGIGEGDYHAGS
                     YVVVPFSVPHGCDGSPTTKLRIKLPESVPTATPTVNPGWTIEIVKEALDTPLDLGEGR
                     TLTERVTEIDYTAITPLPDGFRDVFELSLQLPADAAGSTLTFPTIQECAEGSTEWTQI
                     PEDGQDPEELEHPAPSIAVIESEAEEAAHDHAAEAGSGDTPTTTAASSDDNEAGSKSS
                     DRGLAIAGLATGVIGMGLGGRALVASKGSTGSKGSK"
     gene            1429..3213
                     /locus_tag="PKY13_13965"
     CDS             1429..3213
                     /locus_tag="PKY13_13965"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HOT81095.1"
                     /translation="MIRRQTPRRAVALLAIAFVALLGVVAWPEVAAAHSVLESTSPSS
                     GETVASVPDRIVLQFNEPVNVADGAVRLLDVEGDDVDVPAAKAVDATVTVDLPADLED
                     GSYVVAWKVMSADSHPVSGAYTFAIGAPSVASDGSDPLAAARAEGSGGPGPQIAVDVA
                     ASLAYGGVLLAVGAALFIVFVGAGLSVAPLRRLVTLGAVVGGVGLVAGIAAAAALIDG
                     GWPSGGTLGDEIVGSPGLQALLGVFGLGLLVFAVSRPFPDLVRRIVVIDGTIAALVAF
                     VIVGHTRTASPVALTMAVDVVHLAAAAVWTGGLVALLVALREPSDAGSRSTVVARFSS
                     VATVAIVAVAFAGAVLGWRIVGGWDALTGTTYGVLLLVKVGSFAVLAAVGGYNRFRLV
                     GRVAAEEPAAAVALLRRTLGVEALLVVAIIAVTGSFTSISPESTPAGPDTTPLTCAEV
                     QVMEGMPGMEQMDHSTCTGTTTTGVPFDPTVGAGTGGAVTARDAFGDGLAVVTVSPAT
                     TGRNTVSVTLTDANGNAVDPEEPPTVQFRLRAKDVGPIAAEAERTGPGTYRLVVDFVL
                     PGTWEVNVSAVLSDFEQPQAVVEIRVGS"
     gene            complement(3274..3957)
                     /locus_tag="PKY13_13970"
     CDS             complement(3274..3957)
                     /locus_tag="PKY13_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT81096.1"
                     /translation="MPDHQLPDAVLWDLDGTLVDTEPAWIAAESELAREHGVDWTTED
                     GEAMVGSALPNAAAIMRGRGVHLSDDEIIGFLIERVLVSLGERVPWQPGALELLTELR
                     DAAVPCALVTMSYRDMADRIVAGAPAGVFHAIVAGDEVTQGKPHPEPYLRAAEMLGVD
                     VRRCVAFEDSPTGLAAAEAAGARVVGIQSQLPVASRPGRSRLAALDGFTLADVRRVAA
                     GEVIDQLGG"
     gene            4041..>4566
                     /locus_tag="PKY13_13975"
     CDS             4041..>4566
                     /locus_tag="PKY13_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81097.1"
                     /translation="MASDPHAPVPLALESLTRDELAVLVPEYLLCGHLIDRAGMPHAI
                     SAFGRDGMRDVAIDEWMGASPVYTRRMQRALRFEGDTVETIFKGMQLDVGAPPQFMDF
                     RYRLETPYSGEFWLDHCGALMDVEPMGDDYVVAMCHDIEDPTFDATAIATNLRAQVRP
                     LHRPPRTPADRQPHC"
BASE COUNT          614 a         1622 c         1613 g          717 t
ORIGIN      
        1 gtgctcgaac agcagacgca gaagcgcatc atcgtgttcc gcccgttgtt cgccgttggc
       61 gaccagcagc tggggtacct gccggggagc gagtccgaga agatgtcgcc ctggtcagcc
      121 gcggtgatgg acgcgctgga gtcgatcgcg tcgccgcagg tgatcgacct gatcgtcgcc
      181 gacggcctgc tggaggtgct gccgctcacc cacatccggg ggcgcaccct cgtcgatacc
      241 ttcgtcatcc tggatgaggc ccagcagtat gagcggccgg tcctggtcac cgcgctgagc
      301 cggctcggtg ccgggagccg ggtggtgctc acccacgacg tcgcccagcg cgacaacctg
      361 cgggtcggac gctacgacgg tgtcatgtcg gtcatcgggg cgctcacggg caatccgctg
      421 ttcggccacg tgaccctctc ccggtccgaa cgcagtccga tcgccgccct cgccgcaagc
      481 atcctcgacg tcccggtgcc gtagctgctc ccgcagtctc ggtcgcgatc ggatcccaac
      541 gccgggttcg gtgggcggtc tcggcgcggc gacgggaact tctaacgtgg atccgacgac
      601 catgaggtgt tcgtcggatc gactgaggag tcctcccccc atgcgcacac gtttcaacgg
      661 cctcggccgt gtcctcgtcg gaacgaccgc agtcggagcc ggcctgctgt tggcagcggt
      721 tccggcatcc gcccacgtcg ggatcggcga gggcgactac cacgccggga gttacgtggt
      781 ggtcccgttc tcggtgcccc acggctgcga cggatcgccg acgacgaagt tgcgtatcaa
      841 gctccccgag tcggtgccca ccgccacacc gacggtgaac cccggctgga ccatcgagat
      901 cgtcaaggaa gccctcgaca ccccgctcga tctgggcgag ggtcgtacgc tcaccgaacg
      961 ggtgaccgag atcgactaca cggccatcac gccgttgccc gacggcttcc gcgacgtgtt
     1021 cgaactctcg ctccagctgc ccgccgacgc cgctggctcg accctgacgt tcccgacgat
     1081 ccaggagtgt gcggagggat cgacggagtg gacgcagatc cccgaggacg gtcaggaccc
     1141 cgaggagctc gagcacccgg ccccgtcgat cgcggtgatc gagtccgagg ccgaggaggc
     1201 cgcgcacgat cacgcggccg aggccggctc aggcgacacc ccgacgacga ccgcagcgtc
     1261 ctccgatgac aacgaggctg ggtccaagag ctccgaccgg ggtctcgcga tcgccggtct
     1321 cgccaccggt gtcatcggaa tgggactcgg cggtcgggca ctggtggcct cgaagggctc
     1381 gacggggtcg aagggctcga agtagcgacg tgctctaggt tgcggccgat gattcggcgg
     1441 cagactcctc gtcgggcggt cgccctcctc gccatcgcgt tcgtggccct gctcggggtg
     1501 gtggcgtggc ccgaggtcgc ggccgcgcat tcggtcctcg agtcgacctc gccgtcgtcg
     1561 ggcgagacgg tcgcctcggt acccgatcgg atcgtcctgc agttcaacga gcccgtgaac
     1621 gtcgcggacg gggccgtccg tctcctcgat gtcgagggcg acgacgtcga cgttccggcg
     1681 gcgaaggcgg tcgacgccac cgtgaccgtc gatctcccgg cggatctcga ggacggcagc
     1741 tacgtcgttg cctggaaggt catgtcggcc gactcccacc cggtgagcgg ggcgtacacg
     1801 ttcgccatcg gggcaccgag cgtcgcgtcc gacggatccg atccgttggc cgccgcccga
     1861 gccgagggat cgggcgggcc cggcccccag atcgcggtcg acgtggcagc gtccctcgcg
     1921 tacgggggcg tgttgctcgc cgtcggcgcg gcgttgttca tcgtgttcgt gggggccggg
     1981 ctctcggtgg ctccgctccg tcgcctcgtg acgctcggcg ccgtcgtcgg tggcgtcggg
     2041 ctggtcgcgg ggattgcggc ggcagcggcg ctcatcgacg gggggtggcc ctcgggcggc
     2101 acgctcgggg acgagatcgt gggttcaccg ggtctccagg cgctcctcgg ggtgttcggc
     2161 ctcgggttgc tcgtgttcgc cgtttcccgt cccttccccg acctcgtgcg tcggatcgtc
     2221 gtgatcgacg ggacgatcgc cgccctcgtc gcgttcgtga tcgtcggcca cacccggacc
     2281 gcgtcgccgg tggcgctgac gatggcggtg gacgtcgtcc acctcgcagc ggcggcggtg
     2341 tggacgggtg gcctggtcgc gttgctcgtc gccctgcgcg agccgtccga cgcagggtcg
     2401 cgttcgacgg tcgtcgcgcg gttctcgtcg gtcgcgaccg tcgccatcgt cgccgtcgcg
     2461 ttcgccgggg cggtgctcgg gtggcgcatc gtcggcgggt gggacgcgct gaccgggacg
     2521 acctacggcg tgttgctgct cgtgaaggtc gggtcgttcg cggtcctggc cgccgtgggc
     2581 ggatacaacc ggttccgcct cgtcggccgg gtcgcggcag aggaaccggc ggctgcggtc
     2641 gcgctgcttc ggcggacgct cggggtcgag gcgctcctcg tggtggcgat catcgccgtg
     2701 accggatcgt tcacgagcat ctcgcccgag tcgactcccg ccggtccgga caccaccccg
     2761 ttgacgtgcg cggaggtgca ggtgatggag ggaatgccgg gcatggagca gatggaccac
     2821 agcacgtgca ccggtaccac gacgacgggg gtgcccttcg accccacggt cggagccggg
     2881 acgggcggcg ccgtgaccgc acgtgacgcg ttcggcgacg ggctcgcggt cgtcaccgtg
     2941 tccccggcga ccaccggccg caacaccgtc tcggtcacgc tcaccgacgc caacggcaac
     3001 gccgtcgacc ccgaggaacc accgaccgtc cagttccggc tccgcgccaa ggacgtcggt
     3061 cccatcgccg ccgaggccga gcggactggg cccggcacct accgtctcgt cgtggacttc
     3121 gtgctcccgg gtacgtggga ggtcaacgtg tcggcggtcc tgtcggactt cgagcagccc
     3181 caggccgtgg tcgagatcag ggtcggcagc tgagcgggcc gagtcgccga agtacccacg
     3241 gccgcgtcat cgacgccgtg ccctcggtgc gggtcagccg ccgagctggt cgatgacctc
     3301 gccggcggcg acccggcgga cgtcggcgag ggtgaacccg tcgagggccg ccagacggct
     3361 ccgccccggt cgggacgcga ccgggagctg ggactggatg ccgaccaccc gtgcccccgc
     3421 ggcctcggcc gccgcgagcc cggtggggga atcctcgaac gccacacaac gccgcacgtc
     3481 gacccccagc atctcggcgg cgcgcaggta cggctcgggg tggggtttgc cctgcgtgac
     3541 ctcgtcgccg gcgacgatcg cgtggaacac gccggcgggc gctccggcga cgatccggtc
     3601 cgccatgtcg cgatagctca tcgttaccag cgcgcacggc acggcagcgt cgcggagttc
     3661 ggtgagcagc tccagcgccc ccggctgcca cggcacccgc tcgccgaggg agaccagcac
     3721 gcgctcgatc aggaagccga tgatctcgtc gtcggacagg tgcacgcccc gaccccgcat
     3781 gatcgccgcg gcgttgggca gcgcgctgcc gaccatcgcc tcgccgtcct cggtggtcca
     3841 atcgacgccg tgctctcggg ccagctcgct ctcggccgcg atccaggcgg gctcggtgtc
     3901 gacgagggtg ccgtcgagat cccacaggac ggcatcgggg agctggtggt cgggcatcgg
     3961 acgagtctgc cgacgttcgt cgctcgaccc aaactggaca cctgtctgat ttccccggct
     4021 cgagcatcta cgatgcgcga atggcgtccg atccccatgc acccgttccc ctcgccctgg
     4081 agtcgctcac ccgcgacgag ttggcggtgc tcgtccccga atacctgctg tgcggtcacc
     4141 tgatcgaccg cgccggcatg ccccacgcca tcagtgcctt cgggcgcgac ggcatgcgcg
     4201 acgtcgccat cgacgagtgg atgggcgcga gcccggtgta cacgcgccgg atgcaacggg
     4261 cgctgcggtt cgagggcgac accgtcgaga cgatcttcaa ggggatgcaa ctcgacgtcg
     4321 gcgcgccacc ccagttcatg gacttccgct accggctcga gaccccctac tcgggcgagt
     4381 tctggctcga ccactgcggc gcgctcatgg acgtcgagcc gatgggcgac gactacgtgg
     4441 tcgcgatgtg tcacgacatc gaggacccga ccttcgacgc caccgcgatc gccaccaacc
     4501 tccgcgccca ggtccgcccg ctgcatcgac cgccacgcac gcccgccgac cgtcagccgc
     4561 actgcg
//
LOCUS       DAOSOA010000277         4547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19113_length_4547_cov_5.926981, whole genome shotgun sequence.
ACCESSION   DAOSOA010000277 DAOSOA010000000
VERSION     DAOSOA010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19113_length_4547_cov_5.926981"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="PKY13_13980"
     CDS             <1..1367
                     /locus_tag="PKY13_13980"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT81098.1"
                     /translation="VGDPNVVVTGLTHDSVRVLPGSLFCCVVGERFDGHEFAASAMAA
                     GAAALLVERQLPLDVVQLIVPNVRAAMGPVAAAFWGNPSEGGLQVVGVTGTNGKTTTV
                     TIIGHLLDRLGVRCGIIGTLTGARTTPEAPELQAQLRAFDDAGAQVVAMEVSSHALAL
                     HRVDGTRFALAVFTNIGVDHLDFHETQERYFEAKARLFDERFTSRGLVNVDDVHGRLL
                     RDAGTIDIATYSLADLVDLRLEANSSTFVWRGQSVRLPLAGRFNVSNALAAAEAVVAL
                     GHGPAEVAAALATVAAPPGRFEVVDHGQPFPVIVDYAHTPDALANLLTTARELVAGGE
                     LRVVFGCGGDRDRTKRPQMGAVASELADDVMLTSDNPRSEDPAAIIAAVKSGTTTPVG
                     VEIDRERAIEAVLAASAAGDVVVIAGKGHETTQTIGANVSAFDDRQVAGDWLAAHGWA
                     EPTA"
     gene            1364..2422
                     /gene="mraY"
                     /locus_tag="PKY13_13985"
     CDS             1364..2422
                     /gene="mraY"
                     /locus_tag="PKY13_13985"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT81099.1"
                     /translation="MIRIFLAGGVALVVALIGTKYLIDLLTRLNLGQPIHEDAPEGHT
                     VKAGTPTMGGIAIVFAAVVSYAVSGFYRGIYTQRGLFVVLAITGAGIVGFLDDFIKIR
                     NERNAGLSKRAKMFGLLAVAIAFPILVVTQTKQHTELSFTRFNNFGSWAELGKVGWVI
                     WAVLLIVGSSNAVNLTDGLDGLAAGSSIFAFGAFVVIGFWTFRHPEVYNLASQAELTF
                     GLDLAVIAAAMMGACAGFLWFNAAPAQIFMGDTGALAIGVGLACLALTTNTALLLPII
                     GALFVVETLSVIIQVISFRGFGRRVFLMAPIHHHFEKKGWPETTVIIRFWMLSAACTA
                     AGIGLFYRDFLVLTGTLS"
     assembly_gap    2459..2558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2559..3634
                     /gene="murD"
                     /locus_tag="PKY13_13990"
     CDS             <2559..3634
                     /gene="murD"
                     /locus_tag="PKY13_13990"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT81100.1"
                     /translation="WDDRPLLSITGTNGKTTVTTLVTLMMERSGCRAEAVGNTDVPLV
                     AALADPTIEVFVVESSSFRLARTRRFRPLVATWLNFAEDHLDVHTDLGAYEAAKARQW
                     ADLDATSTAVANAEDPVVARHIGLGGGRFVTFGLSPESARVGTDHYTVRGGGAGGQGG
                     ELVTPSGETIVAATEMFRSMPHDIANALAASATALAGGASLSGVRTALREFTGLSHRV
                     ELVAEADGVTWYDDSKATAPHAVIAAVEGFDRVVLIAGGRNKGLDLGALAGVASRLVG
                     VVGIGEAGPEVVAAFDGVTPAVPTTLASSMAEAVAAAAAMATAGDAVLLSPGCASFDW
                     YENYGQRGDDFAARVRELIGSAP"
     gene            3631..>4547
                     /locus_tag="PKY13_13995"
     CDS             3631..>4547
                     /locus_tag="PKY13_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOT81101.1"
                     /translation="MKGATLTRRRPDSEKSQLRAKRTSVRRTRKQVARAQIPMAFWVI
                     LATVGILCLLGLIMVLSVTTVKSTYEHDTAGYFFNKQAVYLGIGSVLMFVGFKFGYRR
                     IAVFAPAAMAVSLVLLVAVLFLGDKVNGSRRWFDISFFNVQPSEIAKLAVILWASRLL
                     ASRSREMDDWRRTILPVGLGVGATVSLIMLEPDLGTVVVLCFVVFVMLVVAGARLDVL
                     GVATIPVGAILAFVALTGYHADRWGFLNPTMHGDTTNYQLIGSLSSISNGGWFGVGPG
                     ASIAKWGFLPEAHTDAIFAVVAEEMGVIGA"
BASE COUNT          609 a         1514 c         1542 g          782 t
ORIGIN      
        1 tcgtgggcga cccgaacgtg gtcgtcaccg ggctcacgca cgactccgtc cgggtgttgc
       61 ccggcagcct cttctgttgc gtggtggggg agcgcttcga cggccacgag ttcgcggcat
      121 ccgcgatggc ggccggtgcc gccgccctcc tcgtcgaacg acaacttccc ctcgacgtcg
      181 tccaactgat cgttccgaac gtccgggccg ccatgggccc ggtcgctgcc gcgttctggg
      241 gcaacccgtc ggagggtggg ctccaggtcg tcggggtcac gggcacgaac ggcaagacga
      301 cgacggtcac gatcatcggg cacctcctcg accggctcgg tgtccgctgc gggatcatcg
      361 gcacgctcac gggcgcccgc acgacgcccg aggcacccga gctgcaggcg caactacggg
      421 cgttcgacga cgccggtgcg caggtggtgg cgatggaggt gtcctcccac gcgttggcgc
      481 tgcaccgcgt cgacggcacc cgcttcgcgc tcgccgtgtt cacgaacatc ggtgtcgacc
      541 acctcgactt ccacgagacc caggagcgct acttcgaggc caaggcgcgg ctgttcgacg
      601 agcgattcac gtcgcggggt ctcgtcaacg tggacgacgt gcacggtcgc ctgttgcgcg
      661 acgccggcac catcgacatc gccacctact ccctcgccga cctcgtcgat ctccggctcg
      721 aggccaacag ctcgacgttc gtgtggcgag ggcagtccgt gcggctgccc ctggccggcc
      781 gtttcaacgt gtcgaacgcg ctcgcagccg ccgaggcggt ggtggcgctg ggtcacgggc
      841 cggccgaggt cgccgctgct ctcgccacgg tcgccgcgcc tccggggcgg ttcgaggtcg
      901 tcgaccacgg ccaaccgttc cccgtcatcg tggactacgc ccacaccccc gacgcgttgg
      961 ccaatctcct gacgaccgcc cgcgagctgg tcgccggtgg cgagctgcga gtggtcttcg
     1021 gctgcggcgg tgaccgtgac cggaccaagc ggccgcagat gggcgccgtc gcgtccgagc
     1081 tggccgacga tgtgatgctg acttccgata acccccgctc ggaggatcca gcggccatca
     1141 ttgctgccgt gaaaagcggg accaccacgc ctgtgggcgt cgagatcgat cgtgagcgcg
     1201 ccatcgaggc cgtgctggcg gcgtcggcag cgggcgacgt ggtcgtcatt gccggcaagg
     1261 gccatgagac cacccagacc atcggcgcca acgtgtcggc gttcgacgac cgtcaggtcg
     1321 cgggggactg gttggctgcg cacggctggg cggagccgac ggcatgatcc ggatcttcct
     1381 cgccggtggc gtcgcgctcg tcgtcgcgct catcggaacg aagtacctga tcgacctcct
     1441 cacccgactg aacctcggac agcccatcca cgaggacgcg ccggagggcc acacggtcaa
     1501 ggccggtacc ccgacgatgg gcggcatcgc catcgtgttc gccgcggtcg tgagctacgc
     1561 cgtgtcgggg ttctaccgcg gcatctacac ccagcggggc ctgttcgtcg tcctggcgat
     1621 caccggcgcc gggatcgtcg gcttcctcga cgacttcatc aagatccgca acgaacgcaa
     1681 cgccgggctg agcaagcggg ccaagatgtt cggcctgttg gccgtcgcca tcgcgttccc
     1741 gatcctcgtg gtcacccaga ccaagcagca caccgagctg agcttcaccc gcttcaacaa
     1801 cttcggctcg tgggccgagc tgggcaaggt cggttgggtc atctgggcgg tgctcttgat
     1861 cgtcgggtcg tccaacgccg tgaacctcac cgacggtctc gacggcttgg ccgcgggcag
     1921 ctcgatcttc gcgttcggcg ccttcgtcgt catcgggttc tggaccttcc gtcatcccga
     1981 ggtctacaac ctggcgtcgc aggccgagct cacgttcggc ctcgacctcg ccgtgatcgc
     2041 ggcggcgatg atgggtgcct gtgccggctt cctgtggttc aacgccgcgc ccgcgcagat
     2101 cttcatgggt gacacggggg cgttggcgat cggcgtgggt ctcgcgtgcc tcgccctcac
     2161 gaccaacacc gcgttgctgc tcccgatcat cggggcgctg ttcgtggtcg agaccctgtc
     2221 ggtcatcatc caggtgatca gcttccgcgg gttcggtcga cgggtgttcc tgatggcgcc
     2281 gatccaccac cacttcgaga agaaggggtg gcccgagacg acggtcatca ttcggttctg
     2341 gatgctgtcc gccgcctgta cggcggccgg catcggcctc ttctaccgcg acttcctggt
     2401 gctgaccggg acgttgtcgt gacgggaccg gacgcggcgt gagtgccgag cgctcctann
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag tgggacgacc gtccgttgtt
     2581 gtcgatcacg ggcacgaacg gcaagacgac ggtgaccacg ctcgtgacgc tcatgatgga
     2641 gcggtccggg tgccgcgccg aggccgtcgg caacaccgac gtcccgctgg tcgcggcatt
     2701 ggccgatccg acgatcgagg tgttcgtcgt cgagtcgtcg tcgttccgcc tggctcgcac
     2761 ccgccgattc cgtccgctcg tcgcgacctg gttgaacttc gccgaggacc atctcgacgt
     2821 gcacaccgac ctgggcgcct acgaggcggc gaaggcccgc cagtgggccg acctcgacgc
     2881 cacgagcacg gccgtcgcca acgccgagga tccggtcgtc gcgcgccaca tcggtctcgg
     2941 cggcggacgc ttcgtcacgt tcggcctgtc gccggagtcg gcgagggtgg gcaccgacca
     3001 ctacacggtc cggggcggtg gcgccggcgg tcagggcggc gagctggtga ccccgtcggg
     3061 tgagaccatc gtcgcagcga ccgagatgtt ccgttcgatg ccgcacgaca tcgccaacgc
     3121 cctcgcagca tcagccaccg cgctcgccgg aggtgcgagc ctgtcgggcg tgcggacggc
     3181 gttgcgggag ttcacggggt tgtcccaccg ggtcgaactg gtggccgagg ccgacggcgt
     3241 gacctggtac gacgactcga aggcgaccgc cccccacgcg gtgatcgctg ccgtcgaggg
     3301 gttcgaccgc gtggtgctga ttgccggcgg gcgcaacaag ggcctcgacc tgggtgcgct
     3361 ggccggtgtc gcctcccgtc tggtcggggt cgtgggcatc ggcgaggcgg gccctgaggt
     3421 ggtggcggcg ttcgacggtg tcacgccggc cgtgccgaca accctcgcgt cctcgatggc
     3481 cgaggctgtc gccgctgcgg cagcgatggc caccgccggc gacgcggtgc tgctgtcgcc
     3541 cggatgtgcg tcgttcgact ggtacgagaa ctacggccag cgcggcgacg acttcgcggc
     3601 acgggtgcgc gagctcatcg ggagtgcacc gtgaagggcg cgacactgac ccggcgtcgt
     3661 cccgactccg agaagtcgca gctccgggcg aagcggacct cggtccggcg cacccgcaag
     3721 caggttgccc gcgcccagat ccccatggcg ttctgggtga tcctcgccac cgtcggcatc
     3781 ctctgcctcc tcggcctgat catggtcctg tcggtgacga cggtgaagtc cacctacgag
     3841 cacgacacgg ctggatactt cttcaacaaa caggcggtct atctcggcat cggcagtgtg
     3901 ctgatgttcg tcgggttcaa gttcggctac cgccgcatcg cggtgttcgc gccggcggcg
     3961 atggcggtgt cgctcgtgct gctcgtcgca gtgctgttcc tcggcgacaa ggtcaacggc
     4021 tcgcggcgtt ggttcgacat ctccttcttc aacgtgcagc cgtcggagat cgcgaagctc
     4081 gccgtcatcc tgtgggcgtc ccggttgttg gcgagccgca gccgggagat ggacgactgg
     4141 cgccgtacga tcctgccggt cgggctgggt gtgggagcga ccgtcagcct gatcatgctc
     4201 gaacccgacc tgggcaccgt ggtggtgctc tgcttcgtcg tgttcgtgat gctggtggtg
     4261 gccggcgccc gcctcgatgt gttgggggtg gccacgatcc ccgtcggagc gatcctggcg
     4321 ttcgtcgcgc tgaccgggta ccacgccgac cgttgggggt tcctcaaccc gacgatgcac
     4381 ggcgacacca cgaactacca gttgatcggt tcgctctcga gcatctcgaa cggtggctgg
     4441 ttcggtgtcg gtcccggcgc gtcgatcgcc aagtgggggt tcctgcccga ggcgcacacc
     4501 gacgcgatct tcgcggtcgt tgccgaggag atgggtgtca tcggtgc
//
LOCUS       DAOSOA010000278         4544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19137_length_4544_cov_5.191579, whole genome shotgun sequence.
ACCESSION   DAOSOA010000278 DAOSOA010000000
VERSION     DAOSOA010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19137_length_4544_cov_5.191579"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..598)
                     /locus_tag="PKY13_14000"
     CDS             complement(110..598)
                     /locus_tag="PKY13_14000"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOT81102.1"
                     /translation="MTLAGVGYVSAVLLAVIFWAAGSAKLRAPATVAAEFEAMGIRSP
                     ETAAKVLPFLEFGVAILLLAVPWVGAAVALAFLIGFTVVLVRVVRSGAVLSCACFGAT
                     STQPVSWIDLVRNGLLILTAGFGLFAERISPSAPDIVIVLGATAVAVVALRLQRQQRD
                     EV"
     gene            complement(612..2021)
                     /locus_tag="PKY13_14005"
     CDS             complement(612..2021)
                     /locus_tag="PKY13_14005"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOT81103.1"
                     /translation="MSRRRRAGAASMMASGRSERTAELVKLSTVAGANYARKTVGSAF
                     ASDERKAELQARYELQTAEQVAEVLGNMKGAVMKLGQMASYLDGGLPEPVRQALAQMQ
                     QDAPPMSYELVAGVVRAELGGEPDTVFASFEREPMASASIGQVHRAVTHDGRAVAVKV
                     QYPGVDAAIRADLDNSDLLFGAMGMLFSGLDPAPLVAELRDRLLEELDYRNEAANQQR
                     FVDYFAGHPTISVPEVLHELSTGRVLCTELAYGAKWTDVLEWSQEERNLTAETLYRFA
                     FGGIYRLGVFNGDPHPGNYLFRPGGEVTFLDFGLCKVFTPDEIAEFQRLITAMVLHQD
                     PVEVRRVWSEAGVLVDDPKLSDELIHEYFSHFFEMVLKDETMAITSEYASDSLRRFFD
                     LSGPYGEVMKSVTLPSFMVVIQRINLGLYALFGELRAVANWRRLAEEVWPFVDRDPTT
                     PMGEASRRWEIERGYRSAE"
     gene            complement(2087..2647)
                     /locus_tag="PKY13_14010"
     CDS             complement(2087..2647)
                     /locus_tag="PKY13_14010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT81104.1"
                     /translation="MSSSTPAPAADGSSERQTKFPAPMPMTIDPEKSYQATISTSLGD
                     MVAYLHPSKAPKTVNNFVNLARYHYYDGLIFHRVINGFMIQGGCPEGSGRGGPGYRFE
                     DELPQRGQYEIGSLAMANAGPNTNGSQFFIVSGPSGVGLPPSYSLFGKLISGLEVLEE
                     IQKVQTDRSDRPGTDVVINSITIEES"
     gene            complement(2724..4175)
                     /locus_tag="PKY13_14015"
     CDS             complement(2724..4175)
                     /locus_tag="PKY13_14015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT81105.1"
                     /translation="MNDQDLPADDAPASEDVWARLRRLEDLERGPVPAERSAAPPPDR
                     VVPPAKRVAPPDPDHTTVMGTSPAAAPPPADPGVGRQYYVASPTDGGAGPAGAPRPGA
                     AQPAAAPATPSPAAQARAQEQYRAAAQAPAAPTRAPQRTAPSPAAPDSPGGRDRGPSR
                     LRPKAKWFRPKLRWIFLYLPLLVILLLGGVLFWGWNTFNGLYRVELGDALVPATGSAV
                     NYLIVGSDSREGIEGDTPNVGVIGTGTEVKGRRSDTIIVLRVDGDKAEMMSIPRDLWV
                     TNVATGKKGRINGTYAQGPDNLVRSVTANLGIPINRYIEVDFVSFAGMVDAVGGITIN
                     FPHPVIDANSGLKIATAGDNRLDGTQALAYVRSRHYTEEIDGKPVLEPTADLGRQVRQ
                     QNFIRTVLKTVGENRNPLTLAKVGGAAAKGVRVDSEVGFFDLVGLARQLGGANPETKV
                     LPTVGANKGGASVLELKNAEALPILAEFGAGGS"
     gene            4313..>4544
                     /locus_tag="PKY13_14020"
     CDS             4313..>4544
                     /locus_tag="PKY13_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81106.1"
                     /translation="MSTHWRATETDGLPEFAAPQDDAGGDLVLAPLPGLPTRWDALDP
                     SGQPIHHADGAVGNGIEVRNGFIEPVPFDQGPF"
BASE COUNT          699 a         1546 c         1622 g          677 t
ORIGIN      
        1 tggccgagac catcagcgcc ggctgatgcc agggtgaccg cgagttccgg gcgctccagc
       61 ggtacgtatc cgacccgctg gcgcgcccag aactcgagcg agcgacccgt cagacctcgt
      121 cgcgctgctg gcgttgcagg cgcagggcca cgacggcgac cgccgtcgct ccgagcacga
      181 tcacgatgtc gggtgcggac ggcgagatcc gctcggcgaa caacccgaac ccggcggtga
      241 ggatcagcag cccgttgcgc accaggtcga tccacgacac cggctgcgtc gaggtcgccc
      301 cgaagcacgc gcacgacagg acggcgccgg accgaacgac ccgtaccaac accacggtga
      361 acccgatgag gaacgcgagc gccactgccg ctcccaccca cggcacggcc aacagcagga
      421 tcgccacgcc gaactccagg aacggcagca ccttcgccgc cgtctcgggc gagcggatgc
      481 ccatggcctc gaactcggcg gcgaccgtgg ccggcgcccg cagtttggca ctgccggcgg
      541 cccagaagat caccgccagg agcacggcgc tgacgtagcc gacgccggcg agggtcacgt
      601 cacgtcgccg ttcactccgc cgaccggtag ccgcgctcga tttcccagcg ccgggacgcc
      661 tcgcccatgg gggtcgtcgg atcacggtcg acgaacggcc acacctcctc ggccaggcgg
      721 cgccagttgg cgacggcgcg gagctcaccg aacagggcgt acagcccgag gttgatgcgc
      781 tggatgacca ccatgaacga cggcagcgtc acgctcttca tcacctcgcc gtaggggccg
      841 ctcaggtcga agaatcgccg caggctgtcc gacgcgtact ccgacgtgat cgccatggtc
      901 tcgtccttca gcaccatctc gaagaagtgc gagaagtact cgtggatgag ctcgtcggac
      961 agcttgggat cgtcgaccag cacccccgcc tcggaccaca cgcgccgcac ctcgacgggg
     1021 tcctggtgga ggaccatcgc cgtgatgagg cgctggaact cggcgatctc gtcgggcgtg
     1081 aacaccttgc agaggccgaa gtcgaggaac gtgacctcgc caccgggccg gaacaggtag
     1141 ttgccggggt gcgggtcgcc gttgaacaca ccgagccggt agatgccccc gaacgcgaac
     1201 cggtacagcg tctcggccgt gaggttgcgc tcctcctggc tccactccaa cacgtcggtc
     1261 cacttcgccc cgtacgccag ctcggtgcag aggacccggc cggtcgacag ctcgtgcagc
     1321 acctcgggca ccgagatggt ggggtggccg gcgaagtagt cgacgaaccg ctgctggttc
     1381 gccgcctcgt tgcggtagtc gagctcctcc agcaggcggt cgcgcagctc ggcgacgagg
     1441 ggcgccgggt cgaggccgct gaacagcatg cccatggcac cgaacagcag gtcgctgttg
     1501 tcgaggtcgg cgcggatggc ggcgtcgaca ccggggtact gcaccttcac ggccacggcg
     1561 cgcccgtcgt gggtgacggc gcggtgcacc tggccgatcg acgccgaggc catcggctcg
     1621 cgctcgaacg acgcgaacac cgtgtcgggt tcaccgccca gttcggcgcg gaccaccccg
     1681 gcgacgagct cgtagctcat cggcggcgcg tcctgttgca tctgggcaag ggcctgccgc
     1741 accggttcgg gcaacccgcc gtcgaggtag ctggccatct ggccgagctt catgacggcg
     1801 cccttcatgt tgccgagcac ctcggccacc tgctcggcag tctgcagctc gtagcgggcc
     1861 tgcaactcgg ccttccgttc gtccgacgcg aacgccgacc cgaccgtctt gcgggcatag
     1921 ttggcgcccg ccaccgtgct gagcttcacc agctcggccg tgcgctcgct gcgaccggac
     1981 gccatcatcg acgccgcgcc ggcgcgacgt cggcgggaca cgagtgcccc gccgacggtc
     2041 gctgcgccaa tggcgagaac gagagatcgg gctcgcaacg ggtgggtcag gactcttcga
     2101 tggtgatcga gttgatcacg acgtcggtgc cgggacgatc ggagcgatcg gtctgcacct
     2161 tctggatctc ctcgagcacc tcgaggccgc tgatcagctt gccgaacagc gagtacgacg
     2221 gcggcaggcc gacgcccgac gggccggaca cgatgaagaa ctggctgccg ttggtgttgg
     2281 gtccggcgtt cgccatggcg agcgagccga tctcgtactg gccgcgctgg ggcagctcgt
     2341 cctcgaagcg gtagccgggg ccgccgcgac cggagccctc ggggcagccg ccctggatca
     2401 tgaacccgtt gatgacccgg tggaagatca gcccgtcgta gtagtggtag cgggcgaggt
     2461 tcacgaagtt gttgaccgtc ttcggtgcct tcgacgggtg caggtacgcg accatgtcgc
     2521 cgagcgacgt cgagatcgtc gcctggtagc tcttctcggg atcgatcgtc atcggcatcg
     2581 gcgccgggaa cttggtctgg cgctccgagc tgccgtcggc ggccggtgcg ggggtggagg
     2641 agctcatcgt gagacctttc gtgggttgcg gaatgcgcgg gacaacctag cggcaccgaa
     2701 cccagcggag gaggccgaga ccgtcaggat ccgccggccc cgaactcggc gaggatcggg
     2761 agcgcctcgg cgttcttcag ctcgagcacc gacgcgccgc ccttgttggc gcccaccgtg
     2821 gggaggacct tcgtctcggg attggctcct ccgagctggc gggcgagccc gacgagatcg
     2881 aagaacccga cctcggagtc gacccggacc cccttggccg ccgcaccgcc gaccttggcg
     2941 agcgtcaacg ggttgcggtt ctcaccgacg gtcttgagga cggtgcggat gaagttctgc
     3001 tgacgaacct gccggcccag gtcggcggtc ggttcgagga ccggcttccc gtcgatctcc
     3061 tcggtgtagt gccgcgaccg cacgtacgcg agcgcctggg tgccgtcgag gcggttgtcg
     3121 ccggcggtcg cgatcttgag cccggagttc gcgtcgatca ccgggtgcgg gaagttgatc
     3181 gtgatcccgc cgaccgcgtc caccattccg gcgaagctga cgaagtcgac ctcgatgtag
     3241 cggttgatcg ggatgccgag gttggcggtc acgctgcgca ccaggttgtc ggggccctgc
     3301 gcgtaggtgc cgttgatacg acccttcttg ccggtcgcca cgttggtgac ccacaggtcc
     3361 cgggggatgg acatcatctc ggccttgtcg ccgtcgacgc gcagcacgat gatcgtgtcg
     3421 ctgcgtcgac ccttgacctc ggtgccggtg ccgatcaccc cgacgttggg ggtgtcgccc
     3481 tcgatgccct cccgagagtc cgaaccgacg atgaggtagt tgacggcgct cccggtcgcc
     3541 gggaccaggg catcgcccaa ctcgacgcgg tagagcccgt tgaaggtgtt ccacccccag
     3601 aacagcaccc cgccgagcag caggatcacg agcagcggca aatagaggaa gatccaccgg
     3661 agcttggggc ggaaccactt ggccttcggg cgcagccggg acggaccgcg gtcgcggccg
     3721 ccgggcgagt cgggcgcggc cggggacggg gcggtgcgtt ggggtgcccg cgtcggggca
     3781 gcaggcgcct gcgcggcggc gcggtactgc tcctgggcgc gcgcctgggc cgccgggctc
     3841 ggggtggccg gggccgccgc gggctgggcc gctccgggac ggggggctcc ggcgggtccg
     3901 gcccctccgt cggtgggtga ggcgacgtag tactggcgac cgacaccggg gtcggcaggc
     3961 ggaggagccg ctgccggcga ggtgcccatc accgtcgtgt gatcgggatc cggcggggca
     4021 acgcgtttgg cgggaggtac gacccggtcc ggtggcggcg ccgcggagcg ctccgctggg
     4081 acgggaccac gttcgaggtc ctcgagccgg cgcagccggg cccacacatc ctcggaggcg
     4141 ggggcgtcgt cggcggggag atcctggtcg ttcacgccgc aagtctgacc gaccgccgac
     4201 ggtcgagcgg tgcatgggtt ctcaggaacc tgggcgaccg atttcgggtc gacccccgat
     4261 agcctcacct taagtgagtt caacaacacc tcgagtggtt ggcgggagcg agatgtccac
     4321 gcactggcga gcaacggaga ccgacgggct accggagttc gccgcgccgc aggacgatgc
     4381 cggcggcgac ctggtgctgg caccgctgcc ggggctcccg acccgttggg acgcactcga
     4441 cccgtcgggt cagccgatcc accacgccga cggcgctgtc gggaacggca tcgaggtccg
     4501 taacgggttc atcgagcccg tcccgttcga ccaaggaccc ttcg
//
LOCUS       DAOSOA010000279         4524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19250_length_4524_cov_5.934661, whole genome shotgun sequence.
ACCESSION   DAOSOA010000279 DAOSOA010000000
VERSION     DAOSOA010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19250_length_4524_cov_5.934661"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1055
                     /locus_tag="PKY13_14025"
     CDS             72..1055
                     /locus_tag="PKY13_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81107.1"
                     /translation="MSRRFRSIPLTLATATLALVATACPTDPPAPSPYRAELLSPGNR
                     IFVTTSPAGVGTILSNPGGTSDFPADPVTALDLYFDATGLGNPANADRLGRVDHPDGT
                     VSTGIYDGNGVLRIDFGPQTSGYRFSADGSTFGITRWDNTGAYVDIYNAIDLTLHRSF
                     AWPLGTGLPTIQDFSRDGSRLIVRGYNLWSMSEDYALFTATTTGSDPLAIAVPVTGEA
                     KYAYRLTDAGRIVYSVYRGLLRPAFELRTVGVDGLGERSLGLFDSYTGYPETAGEIGG
                     ARVLVEMLVPGTLDRTEIVAVEDSPTATRTVIAGPLDGNSQRFSRLVLAPD"
     gene            complement(1152..1985)
                     /locus_tag="PKY13_14030"
     CDS             complement(1152..1985)
                     /locus_tag="PKY13_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT81108.1"
                     /translation="MTVKEAGQIQGLAKAVLSPVFKFAWRVRTEGLEKVPASGGAILA
                     PNHTSVLDSFFLPLVVGRAMTYVGKAEYMDDWKTKYLFPAMGMIPIDRSGGDASSRAL
                     DAAVGVLERGELFGIYPEGTRSRSGKLHKGHTGVARIALRANAPIVPVGIIGAVEVQP
                     PDAKFPRPFMEVLIRFGDPIDVSRYRDRANDRLVLRQLTDELMYSIRDLSGQEYVDTY
                     ATKTHETMPAESTHIVTPEPNVEALPPLVEKVPSLTTEAAQPRSSTAVLQSRPLVDLS
                     F"
     gene            complement(2170..3243)
                     /locus_tag="PKY13_14035"
     CDS             complement(2170..3243)
                     /locus_tag="PKY13_14035"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT81109.1"
                     /translation="MVSLYTCGITPYDSTHLGHAATYLGYDVLQRRLRDLGHETRCVR
                     NVTDVDDSILAKAREIGVHYLDLAAAEIARFDNDMVTLETIPSYSEPRATSAIPDIRG
                     FIGMVLDEGYAYQAGGAVYFEVSKFPKFGTISNYTRDDMLAFAAERGGNVDDPHKRDP
                     LDFILWQPSLPDEPAWDSLWGPGRPGWHIECSALCLRELGTTIDLHGGGSDLIFPHHE
                     CEAAQSEAATGEPLVRHWMHQAMVSMDGEKMSKSLGNLVFVSDLVKDWDPRAIRLTVV
                     SHHYREPWEWNDSLMPTNDERLRAWVAAGNGSGALDEVRAALDEDLDTPGAIEAIDRA
                     AAKGLGVSEAAALLGVVTDRPVI"
     assembly_gap    3247..3346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3347..4006)
                     /gene="npdG"
                     /locus_tag="PKY13_14040"
     CDS             complement(3347..4006)
                     /gene="npdG"
                     /locus_tag="PKY13_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HOT81110.1"
                     /translation="MQIGVLGGTGPAGSGLAVRLASAGFDVVIGSRSKYRALEVRDKL
                     HAKWEDYNLPLNAGDNEAAALADVVVVATPWDAAAVTVSEVATQLRGKVVISMANALA
                     KVGHEFQPLVPPRGSVAASVQAAVPKSLVAAAMHHVPAKELGDIRHPCISDVLVCSDH
                     PSATATTIDIVSQLPDLRPLDAGELSNAAPIEAFAAVLLQVNMKYKTRVAIRFTGLDD
                     I"
     gene            complement(4075..>4524)
                     /locus_tag="PKY13_14045"
     CDS             complement(4075..>4524)
                     /locus_tag="PKY13_14045"
                     /inference="COORDINATES: protein motif:HMM:NF040046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HOT81111.1"
                     /translation="TIGRRACENEYDALALMGASSPHTEPPAPSAARTVHDRPTAAEL
                     IEAVREFLETDVMGSTEGRVRFHARVAANALAIVERELAAGTAAADGHGWRLAALGVA
                     DDAQLAGAIRAGDLDDRWDDVVTALAATALDKVRVANPRYLRVSDVV"
BASE COUNT          690 a         1519 c         1508 g          707 t
ORIGIN      
        1 ccgagacgag ttggtgacac actgttatgt atcctcctct cacgtagcca ccggcccgga
       61 gtgtgatgac catgtctcgt aggttccgct cgatcccact cacgctggcg acggcaaccc
      121 tcgcgctcgt cgccaccgcc tgcccgaccg atccgccggc accgtccccg taccgtgccg
      181 agctgctgtc gcccggcaac cgaatcttcg tcacgacctc acctgccggg gtcggcacga
      241 tcctctcgaa cccgggcggc acgtcggact tccccgccga cccggtcacg gccctcgacc
      301 tctacttcga cgccaccggt ctgggaaacc cggcgaacgc cgatcgcctg ggtcgcgtcg
      361 accaccccga cgggacggtg tccaccggga tctacgacgg caacggggtc ctccgaatcg
      421 acttcggccc ccagacgtcg gggtaccgct tctccgccga cggctccacc ttcggcatca
      481 cgaggtggga caacacgggc gcctacgtcg acatctacaa cgcgatcgac ctgacgctcc
      541 accgttcgtt cgcgtggccc ctcggcactg gccttccgac gatccaggac ttcagccggg
      601 acgggtcacg actcatcgtc cgcgggtaca acctgtggag catgtcggag gactacgccc
      661 tcttcaccgc gacgaccacc ggcagcgacc cgctcgccat cgcggtaccg gtcacgggcg
      721 aggccaaata cgcctaccgg ctcaccgacg ccggccgcat cgtctactcg gtgtaccgag
      781 gcctgctgcg cccggcgttc gagctgcgca ccgtcggcgt cgacggactc ggcgaacgct
      841 cgctcggact gttcgactcc tacaccggct accccgagac ggcgggcgag atcggcggcg
      901 cccgcgtgct ggtcgagatg ctggtaccgg gcacactcga ccggaccgag atcgtcgccg
      961 tcgaggactc gcccaccgcg acgcgcaccg tgatcgctgg gccactggac ggcaacagtc
     1021 agcggttctc gcgcctcgtc ctcgcacctg actgacgcga ggcggcggcg cgctgagatc
     1081 agccgcggcc gggtcccgat ggcgctgttg gtggccgctg tcgagggccc ggccggggcg
     1141 acgacgtcgg gctagaacga caggtcgacg agcggccggg actgcagcac cgccgtggac
     1201 gaccgcggct gggctgcctc ggtcgtgagc gacggcacct tctcgacgag cggaggcagg
     1261 gcctcgacat tcggttcggg cgtcacgatg tgcgtggact ccgccggcat cgtctcgtgc
     1321 gtcttggtgg cgtacgtgtc gacgtactcc tgaccggaga ggtcgcggat cgagtacatg
     1381 agctcgtcgg tgagttggcg caacaccagt cggtcgttgg cacggtcgcg gtagcgggac
     1441 acgtcgatgg gatcgccgaa acgaatcagc acctccatga acggccgcgg gaacttggcg
     1501 tcgggcggct gcacctcgac ggcgccgatg atgccgacgg ggacgatcgg ggcgttggcc
     1561 cggagtgcga tccgggcgac gccggtgtgg cccttgtgga gcttgccgct gcgggagcgg
     1621 gtgccctcgg gatagatgcc gaacagctca ccgcgttcga gcaccccgac ggcggcgtcg
     1681 agcgcccgcg acgacgcatc tccaccggac cggtcgatcg ggatcatgcc catcgccggg
     1741 aagaggtact tggtcttcca gtcgtccatg tactcggcct tgccgacgta ggtcatggcg
     1801 cgcccgacca cgagcggcag gaagaacgaa tcgagcacgg acgtgtggtt gggggcgagg
     1861 atggcgccgc cggaggccgg caccttctcg agcccctcgg tgcgaacccg ccacgcgaac
     1921 ttgaacacgg ggctcaggac ggccttggca agcccctgga tctgtcccgc ttccttcacc
     1981 gtcatcgtgt cccccttcct cgctcgtctc gttgtctcgt cggtccattg tgctcaagat
     2041 caagcaccga tgctgcgact tccggtgaac aacgtgtgaa ggggctcaga acccaccatt
     2101 tccgagccgt tcctttacga ctgtctaccg cgtgcgaccg gggtcgcgcg tgagaccctg
     2161 tctcagatct cagatcaccg gacggtcggt gacgacaccg agcagggcag cagcctcact
     2221 gaccccgagc cccttcgccg ccgcacgatc gatcgcttcg atggcaccgg gcgtgtcgag
     2281 gtcctcgtcg agcgcggccc gcacctcgtc gagggcgccg ctgccgttgc cggcggcaac
     2341 ccaggctcga aggcgctcgt cgttggtcgg catcagcgag tcgttccact cccacggctc
     2401 gcggtagtgg tgtgagacca ccgtcaggcg gatcgcccga gggtcccagt ccttcacgag
     2461 gtcggacacg aacaccaggt tgccgagcga cttggacatc ttctcgccgt ccatcgacac
     2521 catcgcctgg tgcatccagt gccggacgag tggctcgccg gtcgccgcct cggactgcgc
     2581 cgcctcgcac tcgtggtgcg ggaagatcag gtcggagccg ccgccgtgca ggtcgatcgt
     2641 ggtgccgagc tcacgaaggc acagcgccga acactcgatg tgccaaccgg gacgaccggg
     2701 accccacagc gagtcccacg ccggttcgtc gggcagcgac ggctgccaca ggatgaagtc
     2761 gagcggatca cgcttgtggg gatcgtcgac gttgcccccg cgctcggcag cgaaggccag
     2821 catgtcgtcg cgggtgtagt tgctgatcgt gccgaacttg gggaacttcg agacctcgaa
     2881 gtagacggcg cccccggcct ggtaggcgta gccctcgtcg aggaccatac cgatgaaccc
     2941 gcggatgtcg gggatggcgg acgtggcccg cggctcggag tagctcggga tggtctcgag
     3001 ggtgaccatg tcgttgtcga agcgcgcgat ctccgccgcc gcgaggtcga ggtagtgcac
     3061 cccgatctcg cgggccttgg cgaggatcga atcgtcgaca tcggtgacgt tgcggacgca
     3121 acgcgtctcg tggcccaggt cacgtagccg acgctgcagc acgtcgtagc cgaggtacgt
     3181 cgcagcgtgc ccgaggtgcg tcgagtcgta cggcgtgatg ccacaggtgt acagcgacac
     3241 cacgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcag atgtcgtcga
     3361 ggccggtgaa gcggatggcg acgcgggtct tgtacttcat gttgacctgc agcagcaccg
     3421 cggcgaacgc ctcgatcggt gccgcgttgg agagctcgcc ggcgtcgagc ggacgcaggt
     3481 cgggcagctg ggacacgatg tcgatcgtgg tcgccgtggc cgacgggtga tcggagcaca
     3541 ccagcacgtc gctgatgcag ggatgacgaa tgtcgccgag ttccttggcc ggcacgtggt
     3601 gcatggccgc tgcgacgagc gacttcggca ccgcggcctg taccgaggcg gccaccgaac
     3661 cgcgcggcgg aacgaggggc tggaactcgt ggccgacctt ggcgagcgcg ttggccatcg
     3721 agatgaccac cttgccgcgg agctgggttg caacctccga gaccgtcact gctgccgcgt
     3781 cccacggggt ggcgaccacc acgacgtcgg ccagcgcggc cgcctcgttg tcaccggcat
     3841 tgagcgggag gttgtagtcc tcccacttgg cgtgcagctt gtcgcggact tcgagcgccc
     3901 ggtacttgga ccgcgatccg atgaccacgt cgaagccagc cgaagccagt cgcacggcca
     3961 agccgcttcc tgcaggaccc gtaccaccca agacgccgat ttgcatgagc gagattgtgg
     4021 cgaacgaacc tccgcgacgc caacctgacg gacggcttgc tttctgacgg cccgtcagac
     4081 cacgtcggag acccgcaggt accgggggtt cgcgacgcgg accttgtcga gcgccgtcgc
     4141 ggcgagcgcg gtgacgacgt cgtcccaccg gtcgtcgagg tcgccggcgc gaatcgcgcc
     4201 ggcgagctgg gcatcgtcgg ccacgccgag cgcggcgagt cgccagccgt gaccgtccgc
     4261 ggcggcggtt ccagcggcga gttcccgctc gacgatcgcc agcgcgttcg ccgcgacccg
     4321 ggcgtggaag cggacgcgac cctcggtcga ccccatgaca tcggtctcga ggaactcccg
     4381 caccgcctcg atcaattcgg cggccgtggg gcggtcgtgg acagtgcggg cggctgacgg
     4441 agcgggcggt tcggtgtggg gactgctcgc ccccatcagg gcgagcgcgt cgtactcgtt
     4501 ctcacaggcg cgccggccga tcgt
//
LOCUS       DAOSOA010000280         4509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19321_length_4509_cov_4.651998, whole genome shotgun sequence.
ACCESSION   DAOSOA010000280 DAOSOA010000000
VERSION     DAOSOA010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19321_length_4509_cov_4.651998"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKY13_14050"
     CDS             <1..516
                     /locus_tag="PKY13_14050"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOT81112.1"
                     /translation="ALARRVVELVDDLPVALDDSWGLDHGTWSVLVHVFPGADVPVVQ
                     LSIDGTKPAAWHWDLARRLDPLRDEGVLILGSGNITHNFRELDPGAAVPAAWAVDFDA
                     RIWQAVRDHDRAPLVDYLDLPDGRRAAPTADHYLPLVYVAALARPGESAHEIVSGMDL
                     GSFSMRSFSIS"
     gene            585..1763
                     /gene="argJ"
                     /locus_tag="PKY13_14055"
     CDS             585..1763
                     /gene="argJ"
                     /locus_tag="PKY13_14055"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOT81113.1"
                     /translation="MPESPVPAPSGFRIVTANLGVKDETTDFCLVVSDRPATSAGVYT
                     QSRFAGPSVALSRAATASGAARGVVVISKNANVANGVQGEVDAAELQRLAASAAGIRP
                     GELAVASTGVIGRSYPMDRIRGGIAAVSLPDEDADADAFAAAAEAIMTTDTHPKKATA
                     TIGGPSGVSATLVGIAKGVGMIEPNMATLLTYFFTDAAIPADDLDAVFRRVMATTFNA
                     LSIDSDTSTSDSAVVIANGAAGPVGLEAFEAALHHVALRLVLMIAADGEGASKVIEVE
                     VTGARDTAQAKLVAKSIVNSPLVKTAVHGADPNWGRIVMAIGKCDDQVDIDPERVRVG
                     IGGIEMYPATADLAAAEAHMRGDRVLITADLGIADGAFTAYGCDLTDGYIRINADYTT
                     "
     gene            1760..2617
                     /gene="argB"
                     /locus_tag="PKY13_14060"
     CDS             1760..2617
                     /gene="argB"
                     /locus_tag="PKY13_14060"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOT81114.1"
                     /translation="MTTDQHTNDVHVLLQALPYIQRFAGATIVVKFGGNAMSSPELFE
                     QFAQDIVMMHSVGMRPVVIHGGGPQIGEWLRRMGKDSEFVDGQRVTDAETLEVVQMVL
                     KGKVNSDIVSALNVHGPIAVGLSGQDAGLIEAEERDPSLGFVGDVTRINPSIITRLLA
                     MDLIPVVSTVGSDSDGQAYNINADAVAGAIAEALDAQKLIFLTDVAGLLRDVHDPASL
                     IQRVSTSEAAELIADGTIAGGMIPKIEGCLQAINRGVSEVHLIDGRVPHALLLELFTD
                     AGVGTMVTL"
     gene            complement(2783..>4509)
                     /locus_tag="PKY13_14065"
     CDS             complement(2783..>4509)
                     /locus_tag="PKY13_14065"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT81115.1"
                     /translation="HMRWSSLFIPTLRDTPGDAEAISHQLLVRAGYVRQLHAGHYSLL
                     PLGLRSHTKVANIVREEMDAIGGQEFIHPALHPAAIWQQSGRWETMGQEMFRLVDRKG
                     NDLALGMTHEEVFTIISKELESYKQLPQIWYQVQTKFRDEPRPKAGLLRVREFDMKDS
                     YSFDIDEVGLDVSFQKHHDAYVRIFARLGLPAFPVVASSGAMGGGSSLEFMVPSPAGE
                     DDVAMCPNCDYRANIERATSALPDVAPKDGPDELEKFATPGIVTIAALAKFEGGAGAI
                     HQIKTLVMVLDGEVTLVCLRGDHQLNLQKLQDATGAIDIRPAEEAETVAALGAKPGSL
                     GAVNVSGLTIIADEALRGRFHMTTGANEDGWHWRGVSVERDLAVDRWADLREVTKGEP
                     CINCGAKLDIVKCIEVGHIFKLGTKYSDSLGATVLDADGVLKPLIMGCYGIGVGRNLA
                     AVLETHHDEKGMILPVTIAPYEAVITVVKVNDEESMRVANELYETLRSEGIDVLLDDR
                     DERPGVKFADAELIGIPFRITLGPKGLANGMAEFTPRATRESVDVALGEVAQHTMELV
                     REGRKLGR"
BASE COUNT          701 a         1537 c         1536 g          735 t
ORIGIN      
        1 gccctcgccc gccgggtggt cgagctggtg gacgacctgc ccgtcgcgct cgacgattcc
       61 tggggcctcg accacgggac gtggtcggtg ctcgtccatg tgttccccgg ggcggacgtc
      121 cccgtggtgc agctctcgat cgacggcacc aagccggcgg cgtggcactg ggacctcgcc
      181 cgacggctcg acccgctgcg tgacgagggg gtattgatcc tcgggagtgg caacatcacc
      241 cacaacttcc gcgaactcga cccgggcgcc gcggtgcccg cagcgtgggc ggtcgacttc
      301 gatgcccgga tctggcaggc ggtgcgcgac cacgaccgtg ccccgctcgt cgactatctc
      361 gacctgcccg acggtcgccg tgcagccccg accgcagatc actaccttcc cctcgtctac
      421 gtcgccgccc tggcgcgacc gggggagtcg gcgcacgaga tcgtgtccgg catggatctc
      481 gggtcgttct ccatgcgctc gttcagcatc tcctgagctg acgccagctc atgttgatgc
      541 ggaacactgc atgatcatgc attcgtcgcg ctagggtgac gcccgtgccc gaatcgcccg
      601 tccccgcccc gtccggcttc cggatcgtca ccgccaatct cggagtgaaa gacgagacga
      661 ccgacttctg tctggtggtg tccgaccgcc cggccacgtc ggcaggcgtc tacacccagt
      721 cccgattcgc cggtccgagc gtcgcgctca gccgagccgc cacggcaagc ggggcggcgc
      781 gtggggtggt cgtcatctcg aagaacgcca acgttgccaa cggcgtccag ggcgaggtcg
      841 acgccgccga gctccaacgg ctcgccgcga gcgccgcggg gatccggccg ggggagctcg
      901 cggtcgcgtc gacgggggtc atcggccgga gctatccgat ggatcgcatc cggggtggca
      961 ttgctgcggt gtcgctgcca gatgaggacg ccgacgccga cgccttcgct gcggccgccg
     1021 aggccatcat gaccaccgac acccatccga agaaggccac ggcgacgatc ggcggtccga
     1081 gcggggtcag cgcaacgctc gtgggcatcg ccaagggtgt cgggatgatc gagccgaaca
     1141 tggcgaccct cctcacgtac ttcttcaccg acgcggcgat tcccgccgac gacctcgacg
     1201 cggtcttccg ccgggtcatg gccaccacgt tcaacgcgct ctcgatcgac agcgatacct
     1261 ccacgagcga ttcggctgtg gtcatcgcca acggggccgc cggcccggtc gggctcgagg
     1321 cattcgaggc ggcgctccac cacgtcgccc tccggctcgt gctgatgatc gccgccgacg
     1381 gcgagggagc gtcgaaggtg atcgaggtcg aggtcaccgg tgcccgcgac accgcgcagg
     1441 ccaaactcgt cgcgaagtcg atcgtgaact cgcccctcgt gaagaccgcg gtccacggcg
     1501 ccgaccccaa ctgggggcgg atcgtgatgg cgatcggcaa gtgcgacgac caggtcgaca
     1561 tcgaccccga gcgggtccgc gtcggcatcg gcgggatcga gatgtatccc gcgaccgccg
     1621 atctcgccgc cgccgaggcc cacatgcggg gcgatcgtgt cctcatcacg gccgacctcg
     1681 gcatcgccga cggagcgttc accgcgtacg gttgcgacct caccgacggc tacatccgga
     1741 tcaacgcgga ctacacgaca tgacgaccga ccagcacacc aacgacgtcc acgtcctcct
     1801 ccaggcgctg ccgtacatcc agcggttcgc cggggcgacg atcgttgtga agttcggcgg
     1861 gaacgccatg agctcacccg agctgttcga gcagttcgcc caggacatcg tgatgatgca
     1921 ctcggtcggc atgcgcccgg tggtgatcca cgggggcggc ccgcagatcg gggagtggtt
     1981 gcgccgtatg ggcaaggact ccgagttcgt cgacgggcaa cgggtcaccg atgccgagac
     2041 gctcgaggtc gtgcagatgg tgctcaaggg caaggtcaac agcgacatcg tgtcggcact
     2101 gaacgtgcac ggtccgatcg ccgtgggcct ctcggggcag gacgccggcc tgatcgaggc
     2161 cgaggagcgc gacccgtcgc tcggcttcgt cggcgacgtc acccgcatca atccgtcgat
     2221 catcacccgt ctcctggcga tggacctgat ccccgtcgtg tcgaccgtcg ggagcgactc
     2281 cgacggccag gcgtacaaca tcaacgccga cgcggtcgcg ggtgcgatcg ccgaggcgct
     2341 cgatgcccag aagctcatct tcctcaccga cgtcgccggt ctcctccgcg acgtccacga
     2401 tccggcctcg ctgatccagc gggtctcaac gtcggaggcg gccgagctca ttgccgacgg
     2461 caccatcgcc ggcggcatga tccccaagat cgaaggctgt ctgcaggcga tcaaccgggg
     2521 tgtgtcggag gtgcacctga tcgacggccg ggtcccccac gcgctcctcc tggagctgtt
     2581 caccgacgcc ggtgtcggca cgatggtcac cctgtaggtc gccgatcccg acattcgtcg
     2641 acgagaaact caggttcgga tcgggaaccg ccgacacctg acacgtgctg ggaaacacca
     2701 cgtaaatggg agccggtcga cggcgagcgg tcacactgat gacgctcgcc gtcgccctcc
     2761 tggactggtg ctgccgggcg cgtcagcggc cgagcttgcg gccctcgcga acgagttcca
     2821 tcgtgtgctg ggcgacctcg ccgagggcca cgtcgaccga ctcgcgggtt gcccgaggtg
     2881 tgaactcggc catgccgttg gcaaggccct tggggccgag cgtgatccgg aacgggatgc
     2941 cgatcagctc ggcgtcggcg aacttcacac cgggacgctc gtcgcggtcg tcgagcagga
     3001 cgtcgatgcc ctcgctgcgc agggtctcgt agagctcgtt cgcgacgcgc atcgattcct
     3061 cgtcgttgac cttcacgacg gtgatcaccg cctcgtacgg agcgatcgtg accggcagga
     3121 tcatgccctt ctcgtcgtgg tgcgtctcga gcaccgcagc gaggttgcgg ccgacaccga
     3181 tgccgtaaca ccccatgatc agcggcttga gcaccccgtc ggcgtcgagg acggtcgcac
     3241 ccagcgagtc ggagtacttc gtgcccagct tgaagatgtg accgacttcg atgcacttga
     3301 cgatgtcgag cttcgcgccg cagttgatgc acggctcgcc cttagtgacc tcgcggaggt
     3361 cggcccaccg gtcgacggcc aggtcacgct cgaccgacac gccccgccag tgccaaccgt
     3421 cctcgttggc cccggtggtc atgtggaacc ggccgcgcaa cgcctcgtcg gcgatgatcg
     3481 tcaggcccga gacgttgacg gcgccgagac tgcccggctt ggcgccgagg gcggcgactg
     3541 tctcggcctc ctccgccgga cggatgtcga tggccccggt cgcatcctgg agcttctgga
     3601 ggttgagctg gtggtcgccc cggaggcaca cgagcgtgac ctcaccgtcg agcaccatga
     3661 ccagcgtctt gatctggtgg atggcgccgg cgccgccctc gaacttggcg agcgccgcga
     3721 tggtgacgat gccgggggtg gcgaacttct cgagttcgtc gggaccgtcc ttcggggcga
     3781 cgtcggggag cgcggacgtg gcccgctcga tgttggcccg gtagtcgcag ttggggcaca
     3841 tggcgacgtc gtcctcaccg gcgggggagg gcaccatgaa ctcgaggctc gagccgccac
     3901 ccattgcgcc cgacgaggcg acgacgggga acgccggcag gccgaggcgg gcgaagatgc
     3961 ggacgtaggc gtcgtggtgc ttctggaacg agacgtcgag tccgacctcg tcgatgtcga
     4021 agctgtagga gtccttcatg tcgaactcgc ggactcggag caggccggcc ttggggcgcg
     4081 gttcgtcgcg gaacttcgtc tgcacctggt accagatctg ggggagctgc ttgtaggact
     4141 cgagctcctt cgagatgatc gtgaagacct cttcgtgggt catgccgagc gcgaggtcgt
     4201 tgcccttgcg gtcgacgagc cggaacatct cctggcccat cgtctcccac cggcccgact
     4261 gctgccagat ggcagccggg tgcagggccg ggtggatgaa ctcctggccg ccgatggcgt
     4321 ccatctcctc gcggacgatg ttcgccacct tggtgtgcga ccgcagcccc agcggcagga
     4381 gcgaatagtg gccggcgtgc agctggcgga cgtagcccgc tcgcaccagc agttggtggc
     4441 tgatcgcctc ggcgtcgccg ggggtgtcgc ggagtgtggg gatgaacagg ctggaccaac
     4501 gcatatgcc
//
LOCUS       DAOSOA010000281         4494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19396_length_4494_cov_5.187204, whole genome shotgun sequence.
ACCESSION   DAOSOA010000281 DAOSOA010000000
VERSION     DAOSOA010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19396_length_4494_cov_5.187204"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PKY13_14070"
     CDS             <1..586
                     /locus_tag="PKY13_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81116.1"
                     /translation="VLAWENFNGTTGANIGGTTTDGGAKTWAVNVSGGVWQINANRAR
                     STTADTSLVLNAGGWNATNVATVARNGATAFDAGFTTNRNAAGTQFLTCEWTSSSNGS
                     MELWRYNSGWTLLTSVTNLYPGGIATAPASITFGCGTTATTLTATINGTAVASTTLSA
                     GDQTTFKNSTHQLFGLYQYTSTGITWDDFHLDSP"
     gene            complement(1111..2316)
                     /locus_tag="PKY13_14075"
     CDS             complement(1111..2316)
                     /locus_tag="PKY13_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT81117.1"
                     /translation="MTVTDESATATDPDAARVDALIDQLLAENPPESTDDQAFLEAQY
                     DLGLAWVRFPEGYGGIGVSPKLQRTVISRVNRAGGPVGGTLNPIGYGMCAPAVMEHGT
                     DEQKRMLRALFSNEDIWCQLFSEPGAGSDVASLACKAERDGDEWVLNGQKVWTTLAHI
                     SNYGLIIARTDPEQAKHRGMTAFIIDMHAPGVEVRPLRQITGEAEFNEVYFTDVRVPD
                     TMRVDEVGRGWAVSITTLMNERVSIGGGTPPRNSGPIGQLLDLWKRTPDTTAVQRDEF
                     MRLWSQAEINRLTNIRASQNAKLGTPGPEGSIAKLAMADLNKLIYSFAIDLLGASGML
                     FDSYAMHQPKTALFGDDIAKNFLRARANSIEGGTTEVMKNIVGERVLGLPGDIRVDKD
                     LPWSKVPRS"
     gene            complement(2402..3190)
                     /locus_tag="PKY13_14080"
     CDS             complement(2402..3190)
                     /locus_tag="PKY13_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOT81118.1"
                     /translation="MDASEEFRNEVELGGAAALDRLALLIAAKGAGPLDVSGEQARLD
                     ELATDFGGGTAAELMGWLRIRGFRGNADSYYDPDNSFLNRVLDRGLGIPISLSVLAIE
                     VGRRCDIHLVGIGLPGEFIVAERDEPDRFHNPFRHQSMSPAQVTELLRRFAGPAARLT
                     PDMVQPVGALSIAARMLANLAHIYAHTARPADLEWVLRFQTTLPGAAPAVRRQLVATL
                     AALGRFWEASDVLAALVADPLAPATPDEREADETLLTQLRGNLN"
     gene            3344..4291
                     /locus_tag="PKY13_14085"
     CDS             3344..4291
                     /locus_tag="PKY13_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81119.1"
                     /translation="MPVNALCVVSELAASQHNVFTRSQAAAEGVRRDHLRRLIIARTV
                     IAMPSGGLVLLAQDGQPSWLQRVTGAVLDHPGTFASHTTAARLDGTDGYDVFDEIELS
                     VLRPRVLRIPGVTVHRVHALDRCDTFPIGAVPCTNRARTLCDLGNSVEPAMVRRALQD
                     ALRRGANERWLRATAERLHRPGQRGTAELVRQLDAHAERGRVPDSWFEQLLDDCLDDP
                     RIGLVERQHELRDDDNRLVARFDLAVPEVKLGIEAHSRKHHFLEEEEASDEHRDHAAA
                     ELGWDVAYLGYSALESPSSVARRIARRVEVRRAQLGSGA"
BASE COUNT          681 a         1565 c         1542 g          706 t
ORIGIN      
        1 ggtgctggcg tgggagaact tcaacggaac caccggagcc aacatcggtg gcaccaccac
       61 cgacggcggc gcgaagacct gggcggtcaa cgtcagcggc ggtgtgtggc agatcaatgc
      121 gaaccgtgcc cggtccacca cggccgacac ctcgctcgtc ctgaacgccg gcgggtggaa
      181 cgccaccaat gtcgccaccg tcgcccgcaa tggggcgacg gccttcgacg ccgggttcac
      241 cacgaaccgc aacgcggccg gaacgcagtt cctcacatgt gagtggacgt cgtcgagcaa
      301 cggctcgatg gagttgtgga ggtacaactc cggatggacg ctgctcacgt cggtgaccaa
      361 cctctacccc ggcggcatcg ccacggcgcc ggcctcgatc accttcgggt gtggcacgac
      421 cgcgaccacg ctgacggcga cgatcaacgg caccgccgtc gcgagcacca cgttgtcggc
      481 cggcgaccag accacgttca agaactcgac gcaccagttg ttcgggctct accagtacac
      541 gtcgaccggt atcacgtggg acgacttcca cctggactcg ccatgagcca accagtcgaa
      601 ccagattccg gccccggcgt ccggcaggag cgtcgcgtac gagcccgccg tcgtcgcccc
      661 gtctcgacgt tgctgttcgc cggcgcggtg gccctgttcc tcgtggtcct gtggccggcc
      721 cgcttcggcg gcgcagcatc gttcgtcgtc gtgcgcgggc attcgatgga gccgacctac
      781 gcgctcggcg acctcgtgta cgtccgctcc gccgacgact accggcccgg cgagatcgtc
      841 gtctaccggg tccccgacga acaacccggc gccggcatct tcatcgtcca tcgcctgcgc
      901 cgcatcgaac ccgacggcac gttcgtcttc cgcggggaca acaacccgac cgacgacgtg
      961 ttccggccga cgcgccacga catcgtcggg cgcgccgtgg tcgacctcgg cggattccct
     1021 gcccgggcga tgtcgatcgc tccgctgatc ggcgcactcg ccgtcgccgt cgccgtcggg
     1081 ctgctcatgt ggcccgaccg agaccccgac tcagctccgg gggaccttgc tccaggggag
     1141 gtccttgtcg acacggatgt cgccggggag tccgaggact cgctcgccga cgatgttctt
     1201 catgacctcg gtggtgccac cctcgatcga gttggcccgg gcccgcagga agttcttcgc
     1261 gatgtcgtcg ccgaagagcg ccgtcttggg ctggtgcatg gcgtaggagt cgaacagcat
     1321 gcccgacgcg cccaacaggt cgatggcgaa cgagtagatg agcttgttga gatcggccat
     1381 cgcgagcttg gcgatcgacc cctcggggcc gggggtgccc aacttggcgt tctgcgaggc
     1441 ccggatgttc gtcagccggt tgatctcggc ctgcgaccag agccgcatga actcgtcacg
     1501 ctgcaccgcg gtggtgtcgg gcgtgcgctt ccagaggtcg agcagctggc cgatcggacc
     1561 gctgttgcgg ggcggggtgc cgccaccgat tgagacccgc tcgttcatga gggtcgtgat
     1621 cgagaccgcc cagccgcggc cgacctcgtc gacacgcatc gtgtcgggga cccgaacgtc
     1681 ggtgaagtac acctcgttga actccgcttc gccggtgatc tgacggaggg gacggacctc
     1741 gacgccgggg gcgtgcatgt cgatgatgaa cgccgtcatg ccgcggtgct tggcctgctc
     1801 ggggtcggtg cgggcgatga tcaggccgta gttggagatg tgcgccaagg tggtccacac
     1861 cttctggccg ttgaggaccc attcgtcgcc gtcacgttcg gccttgcagg cgagcgacgc
     1921 gacatcggag ccggcgccgg gctcgctgaa cagctggcac cagatgtcct cgttgctgaa
     1981 cagcgcccgg agcattcgct tctgctcgtc ggtgccgtgc tccatgaccg ccggggcgca
     2041 catgccgtag ccgatcgggt tgagcgtgcc gccgacgggg ccaccggcgc ggttcacgcg
     2101 cgagatgacg gtgcgctgca gcttggggct gaccccgata ccgccgtagc cctcggggaa
     2161 ccggacccag gcgaggccga ggtcgtactg ggcctcgagg aaggcctggt cgtcggtcga
     2221 ctcaggcggg ttctcagcca gcaactggtc gatcagcgcg tcgacgcgcg ccgcgtcggg
     2281 atcggtggcg gtcgcgctct cgtcggtgac ggtcatctgt tccccctgga cgtgtcgatg
     2341 atcggcgcag cctacgagcg cggaccgcac ttgaccagtc ggtcaattgg agggcggatc
     2401 cctagttgag gttgccgcgg agctgggtga gcagcgtctc gtcggcctca cgctcgtcgg
     2461 gcgtcgccgg cgcgagcggg tcggcgacga gcgctgcgag cacgtcggac gcctcccaga
     2521 accggccgag cgcagcgagc gtcgccacca gctgacggcg caccgccggc gccgcccccg
     2581 ggagggtcgt ctggaatcgc aacacccatt cgagatcggc gggccgggcc gtgtgggcgt
     2641 agatgtgggc caggttcgcg agcatccgag ccgcgatcga cagcgccccg accggctgga
     2701 ccatgtcggg cgtgagccgg gcggccgggc ccgcgaaccg ccggaggagt tcggtgacct
     2761 gcgccggcga catcgactgg tgccggaacg ggttgtggaa ccggtcgggt tcgtcacgct
     2821 ccgcgacgat gaactcgccg gggagcccga tgccgacgag gtggatgtcg cagcgccgac
     2881 ccacctcgat cgccagcacg ctcaggctga tcggaatgcc cagaccgcgg tcgagcacgc
     2941 gattgaggaa ggagttgtcg gggtcgtagt acgaatcggc gttgccgcgg aatccccgaa
     3001 tacgcagcca gcccatcagc tccgccgcgg tgccgccgcc gaaatcggtg gcgagttcgt
     3061 cgagtcgagc ctgctcgcca ctcacgtcga gcggacccgc ccccttcgcc gcgatgagca
     3121 gggccagccg atcgagggcc gccgctccac cgagctccac ctcgttccgg aactcctcgc
     3181 tcgcgtccat ccgtgaaacc tacgccgcag ccgccccgac accgggtctg cacggggccg
     3241 ccgccggagg aagtggtgcg ccagcgcgac gtatccatcg cgctcaggca ccacttcgcc
     3301 gccgagtgtc tggatgtcgg gggtacccac caagatggac gccatgccgg tcaacgcctt
     3361 gtgcgttgtg tccgagctcg cagcctctca acacaacgtc ttcacccgtt cccaggctgc
     3421 tgccgaagga gtccgcaggg atcacctgcg ccgactgatc atcgcaagga cggtgatcgc
     3481 catgcccagc ggcgggctcg tgctgctcgc ccaggacggt cagccgtcgt ggctgcaacg
     3541 cgtgaccggt gccgtgctcg accaccccgg cacattcgcc tcccacacga ccgctgctcg
     3601 gctcgacggc accgacgggt acgacgtgtt cgacgagatc gagctgagtg tgctccggcc
     3661 tcgggtcctc cgcattcccg gcgtgaccgt gcaccgtgtg catgctctcg atcgctgcga
     3721 caccttcccg atcggcgcag tgccgtgcac gaatcgggcg cgcacgctct gcgacctcgg
     3781 gaattcggtc gagccggcga tggtgcgtcg cgccctccag gacgcactgc gtcggggcgc
     3841 gaacgagcgg tggctccggg caacggctga gcgccttcac cgcccgggcc aacgcgggac
     3901 ggccgaactc gtccgacaac tcgacgccca cgccgagcgg ggcagggtcc ccgactcgtg
     3961 gttcgagcag ctcctcgacg actgcctcga cgacccccgg atcgggctcg tcgagcggca
     4021 gcacgagttg cgggacgacg acaaccgctt ggtcgcccgg ttcgatctcg ccgtgccgga
     4081 ggtgaagctc gggatcgagg cgcacagtcg caagcaccat ttcctcgagg aagaggaggc
     4141 cagcgacgaa catcgtgatc acgccgccgc cgaactcggg tgggacgtgg cgtacctcgg
     4201 gtacagcgcc ctcgagtcgc cctcgtcggt ggcccgccga atcgctcggc gcgtcgaggt
     4261 ccgccgggct cagctcggaa gtggtgcctg agcgcgatgg atgcgtcgcg ccggcgcacc
     4321 acttctccgc ctgcggggtc gtcgcgggac cactagacac tgggggatgg ctccccggtt
     4381 cgcgttcctc gaccacgacg gaccgatcgc gttcgcccac cgcggcgggg ccggcgcgtg
     4441 gccggagaac acgatgccgg ccttccaggg ggccgtcgac ctcggctacc gcta
//
LOCUS       DAOSOA010000282         4478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19493_length_4478_cov_7.211395, whole genome shotgun sequence.
ACCESSION   DAOSOA010000282 DAOSOA010000000
VERSION     DAOSOA010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19493_length_4478_cov_7.211395"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKY13_14090"
     CDS             complement(<1..766)
                     /locus_tag="PKY13_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT81120.1"
                     /translation="MTERATPTTASTDDASPAIVCDGITRSFDDGIAVDHLSFEVPRG
                     SVLALLGNNGAGKTTTIRLLNGVLRPDSGTCRVLGLDPTTRGTDVRRRTGVVTENAGL
                     DDRLTARENLAITARLRGIRPAVARARVDDLLERFDMTSHADQACQGFSTGQRRRIAL
                     ARALVHDPELLFLDEPTSGLDPAGARAVMDLIETSARDRGRTIVLCTHFLSEAGTSAD
                     LMAVMHRGRLLSFGRPEALAAERWPGLEVHLDLGASP"
     gene            complement(759..1472)
                     /locus_tag="PKY13_14095"
     CDS             complement(759..1472)
                     /locus_tag="PKY13_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT81121.1"
                     /translation="MRRAVRALCIAVAATAAIATGLAAPSLSPAGASTATATSRSQEW
                     GAAEEQAFVDKINQLRASLGLPTLAVDPELAAQARVWSQTMKDAGNIFHSKALDAGIS
                     ADWEKLGENVGVGDTVDALFDAFVNSPKHYENLVDPSFRFIGVGVVWDGGRMFTAHRF
                     MALMPPAPAPTATNSRPSTTPRTAAPANPADPAPDELAATEPPAAEPPAPSPPPPTVP
                     PASPERVALILDTIDDLLD"
     gene            1655..>3117
                     /locus_tag="PKY13_14100"
     CDS             1655..>3117
                     /locus_tag="PKY13_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT81122.1"
                     /translation="MNLATIFDRHPDDAVAFISRGKNTTYGELRGHVAGYRAGLAEMG
                     LEPGDRLAIICGNNWYFVAAYLAALGAGLVAVPLNPISPPLELSAELTAVGAKGVVIG
                     PTARTMFETLDRSTIPSVEAFIGCGFVPDGGVSHEDLVAREGVDIVERDDTDLATLIF
                     TSGTAGSPKAAMLTHGNLRANIAQVLSTPGNAQSADDVVFAVLPMFHIFGLNVVLGVT
                     FAVGARLLLVERFDPISAIESIQKHQATIVTGPPTMWAAWAGLPEAPEHAFDSVRLAV
                     SGAARLPDEVARTMQRRYDVHLTEGYGLTEASPVVTSAVGTDAPVGSVGAPVPGLELR
                     LVDEDGDDVLIGDSGELWVRGPNVFAGYWNDEDATARAINPEGWLVTGDVATVDDGGY
                     LYLVDRVKDLIIVSGFNVYPAEVEEVLMQHPAIDACAVVGVPHPYSGEAVKAYVVTAD
                     GQSIEEDEVVRFCSSRLARYKCPEKVWFVDEVPQGMGG"
     assembly_gap    3118..3217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3262..3945
                     /locus_tag="PKY13_14105"
     CDS             3262..3945
                     /locus_tag="PKY13_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOT81123.1"
                     /translation="MAGSPQGQSIPEATVSRLPLYLRSLQELMDEQLATVSSERLAEM
                     AGVNAAKVRKDLSYFGSYGTRGVGYDVEYLLLHIRRELGLTHDWPCVIAGMGNLGQAL
                     ANYGGFSERGFVIAALVDANPAKVGTELEGVRISHVDELGELVSGLGITIGVIATPAH
                     AAQEVANSMVEAGITSILNFAPTVITVPDGILVRKVDLAVELQILSFYQERRSGGSPT
                     TPGLASAGS"
     gene            4052..>4478
                     /locus_tag="PKY13_14110"
     CDS             4052..>4478
                     /locus_tag="PKY13_14110"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81124.1"
                     /translation="MSTVVAIGATHRSAPLALLERLTIDDTLLPKYLDDLVSRDHINE
                     AVIISTCNRIEIFVTAEKFHGAYRDVRDFISDVTFTLPEEFADHLEVSHDADAVRHLF
                     AVAAGLESVVVGEHEILGQVRNAWTTARDAGVAGTTLNLL"
BASE COUNT          650 a         1494 c         1535 g          699 t
ORIGIN      
        1 acggcgacgc gccgaggtcg aggtgcactt cgaggcccgg ccagcgctcg gccgcgagcg
       61 cctccggccg cccgaacgac agcagccggc cacggtgcat gacggccatc aggtcggcgc
      121 tcgtgcccgc ctcggagagg aagtgggtgc acaggacgat cgtgcgaccg cggtcacggg
      181 cactggtctc gatcaggtcc atcacggcgc gggcgccggc cggatcgagg cccgaggtcg
      241 gctcgtcgag gaagaggagc tcggggtcgt ggacgagcgc gcgagccaag gcgatccggc
      301 gtcgctggcc ggtcgagaag ccctggcacg cctggtcggc gtgggacgtc atgtcgaacc
      361 gctcgagcag gtcgtcgaca cgggcccggg cgacggccgg ccggatgccg cggagtcggg
      421 ccgtgatggc gaggttctcc cgtgccgtca gccggtcgtc gagtcccgcg ttctcggtga
      481 ccacaccggt gcggcggcgc acgtcggtcc cgcgggtcgt cggatcgagg cccagcacgc
      541 ggcaggtgcc ggagtcgggc cggaggacgc cgttgaggag gcggatggtc gtcgtcttgc
      601 cggcgccgtt gttcccgagg agcgcgagca ccgaaccgcg gggcacttcg aacgagaggt
      661 ggtccaccgc aatgccgtcg tcgaaggatc gggtgatccc gtcacacacg atcgcggggg
      721 aggcgtcgtc ggttgacgcc gtcgtggggg tagcgcgctc agtcaaggag gtcgtcgatc
      781 gtgtcgagga tgagcgccac ccgttccggc gacgccggtg gaaccgtcgg tggcggagga
      841 ctgggcgccg gcggctcggc ggccgggggt tcggtcgcgg ccaactcgtc gggcgccggg
      901 tccgccgggt tcgcgggtgc cgcggtgcgc ggcgtggtcg agggccggct gttcgtcgcc
      961 gtcggggcgg gcgccggggg catcaacgcc atgaagcggt gcgccgtgaa catccgcccg
     1021 ccatcccaca ccacgccgac gccgatgaac cggaagctcg ggtcgacgag gttctcgtag
     1081 tgcttcggac tgttgacgaa tgcgtcgaac aaggcgtcga ccgtgtcccc gacgccgacg
     1141 ttctcgccca gcttctccca gtcggcggag atcccggcgt cgagggcctt gctgtggaag
     1201 atgttgccgg cgtccttcat ggtctgcgac cacacgcggg cctgcgctgc gagctcgggg
     1261 tcgaccgcca acgtcggcag tccgagcgac gcccgcagct ggttgatctt gtcgacgaag
     1321 gcctgttcct cggcggcccc ccactcctgg gagcgggagg tcgcggttgc ggtgctggca
     1381 ccagccggcg acagggacgg cgcggccaac ccggtggcga tcgccgcggt cgctgccacg
     1441 gcaatgcaga gtgctcgcac cgcacgacgc atgtcgttcg tctccgatcc gtcggccctg
     1501 ccccaacgcg aggccgttcc cttttccttc ggcgagctcg cgggcgagct tgaacaagcc
     1561 tccggaggcg accgaacccg gtccggttcc gcccggggca cgtcagggga tgcggcgcta
     1621 cccttggcgc agtccgctcc ccagaggaga acgagtgaac ctggcgacga tcttcgatcg
     1681 gcaccccgac gatgcagtgg cgttcatcag ccgcggcaag aacacgacct atggggagct
     1741 gcgcgggcac gtcgccggat accgggccgg cctcgccgag atgggcctcg agcccggtga
     1801 ccgcctcgcg atcatctgcg gcaacaactg gtacttcgtc gcggcctacc tcgcggcgct
     1861 cggcgccggt ctggtcgccg tgccgctcaa cccgatcagc cccccgctcg aactctcggc
     1921 cgagctgacc gcagtcgggg ccaaaggtgt cgtcatcggc ccgacggcgc gcacgatgtt
     1981 cgagaccctc gaccggtcca cgatcccgag cgtcgaggcg ttcatcggct gcggattcgt
     2041 gcccgacggt ggggtgagcc acgaggacct cgttgcccgc gagggcgtcg acatcgtcga
     2101 gcgcgacgac accgacctgg cgacgctcat cttcacgagc ggcaccgccg ggtcacccaa
     2161 ggcggcgatg ctcacgcacg gcaaccttcg cgccaatatc gcccaggtgc tgtccacgcc
     2221 cggcaatgcc cagagtgccg acgacgtggt gttcgcggtg ctgccgatgt tccacatctt
     2281 cgggctgaac gtggtgctcg gggtgacgtt cgccgtcggc gcccggttgt tgctcgtcga
     2341 gcgcttcgac ccgatctccg cgatcgagtc catccagaag caccaggcga cgatcgtcac
     2401 cggcccgccc accatgtggg cggcgtgggc cggccttccc gaggcgcccg aacacgcctt
     2461 cgattccgtg cgtctcgccg tctcgggcgc cgcccgtctg cccgacgagg tcgcccgcac
     2521 gatgcagcgc cgctacgacg tccacctgac cgagggctac gggctcaccg aggcatcgcc
     2581 ggtggtcacg tcggccgtgg gaaccgacgc ccccgtcggg tcggtcggtg cgccggtccc
     2641 tggtctcgag cttcgcctcg tcgacgagga cggcgacgac gttctgatcg gtgactccgg
     2701 cgagctgtgg gtccggggcc cgaacgtctt cgccggctac tggaacgacg aggacgccac
     2761 cgcccgtgcg atcaaccccg agggctggct ggtgaccggt gatgtcgcca ccgtcgacga
     2821 cgggggctac ctgtacctcg tcgaccgagt gaaggacctc atcatcgtgt cggggttcaa
     2881 cgtgtacccc gccgaggtcg aagaggtgct gatgcagcat ccggcgatcg acgcctgcgc
     2941 ggtggtcggc gtgccccacc cctactcggg tgaggccgtc aaggcgtacg tcgtcaccgc
     3001 cgacggacag tccatcgagg aggacgaggt cgtacggttc tgttcgagcc gcctcgcccg
     3061 ctacaagtgt cctgagaagg tgtggttcgt cgacgaggtg ccccagggaa tgggtggnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg cactttgtga aataacgcac
     3241 gtggacggta ccctcggtcg aatggctggg agcccgcaag gccaatcgat ccccgaagcg
     3301 accgtgtcgc ggctccctct ctacctgcgg agcctgcagg aactgatgga cgagcagctc
     3361 gccacggtgt cgtcggagcg cctggccgag atggccggcg tcaacgctgc caaggtgcgc
     3421 aaagacctct cctacttcgg cagctacggc acccgcggcg tcgggtacga cgtcgagtac
     3481 ctcctgttgc acatccgacg tgaactgggg ctcacccacg actggccgtg tgtcatcgcc
     3541 ggcatgggca acctcggcca ggcgctcgcc aactacggcg ggttctccga gcggggcttc
     3601 gtgatcgctg cgctggtcga tgccaacccg gccaaggtcg gcaccgagct cgaaggggtt
     3661 cgcatcagtc acgtcgacga gctcggcgag ctggtgtcgg gcctcggtat cacgatcggt
     3721 gtgatcgcca cgcccgccca cgcagcgcag gaggtggcga acagcatggt ggaggcgggc
     3781 atcacgtcga tcctcaactt cgcgccgacc gtgatcacgg tgcccgacgg catcctcgtt
     3841 cggaaggtcg acctggccgt cgagctgcag atcctcagct tctaccagga gcggcgttcg
     3901 ggtggctcgc cgaccacccc aggcctcgcc agcgccggct cgtgagcgat cggtcacact
     3961 cctcgggccc agcttggcgt tctgccgggc gatcggtgaa actggccagt accttgggcg
     4021 tacgtcccgg gacgaaagct caggtgtgtc cgtgtcaacc gtcgtagcga tcggtgcaac
     4081 tcatcgcagc gccccgcttg cgttgctgga gcggttgacg atcgacgaca ccctgttgcc
     4141 gaagtacctc gacgatctgg tctcgcgtga ccacatcaac gaggccgtga tcatctcgac
     4201 gtgcaaccgc atcgagatct tcgtgaccgc cgagaagttc cacggcgcct accgcgacgt
     4261 ccgtgacttc atcagcgacg tgaccttcac gttgcccgag gagttcgcgg atcacctcga
     4321 ggtctcccac gatgccgacg ccgtccggca tctcttcgcc gtcgccgccg gtctcgagtc
     4381 ggtggtcgtc ggcgagcacg agatcctcgg tcaggtccgc aacgcgtgga cgacggcacg
     4441 cgacgccggt gtcgccggca cgacgctcaa cctgctgt
//
LOCUS       DAOSOA010000283         4450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19646_length_4450_cov_7.186803, whole genome shotgun sequence.
ACCESSION   DAOSOA010000283 DAOSOA010000000
VERSION     DAOSOA010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19646_length_4450_cov_7.186803"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1392)
                     /locus_tag="PKY13_14115"
     CDS             complement(43..1392)
                     /locus_tag="PKY13_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT81125.1"
                     /translation="MTDVAPAASENTDAVTNDELVDELRSWLEENWDPDLTVADWWQR
                     LGLAGWCAPNLPTNAYGKGVSRNDAVRIANEIGDFGALGAPAGLGLLLAAPTIASHGT
                     QEQIDLYVRDIVTGQKAWCQLFSEPGAGSDLAGLTARAVEDGDEWIVNGQKVWTSFGH
                     TADLAMLIARTAPELPKHQGITWFSIDMHQPGMEVRPLREMTGHALFNEVFMSDARVP
                     SSAIIGGRNNGWAVANTTLANERAGLGTGGGSGGAGAASPGTVVGHLDQRVGDFVTPR
                     QKKKRATSGAGPSSGGGAARLLIDLAKGNGSIADATIRQDLMRLHTLGEIGRFNALRV
                     KAAKKAGRDLPGMANISKLSMSDTMRLSRDVGLRIVGAQGMLHAYTDDQRPAIDAATG
                     NPFLGAVTSIALWAQGPPIYGGTDQIQRNIIGERVLGLPKEPNNDKTMPFADLPKNA"
     gene            complement(1577..2494)
                     /locus_tag="PKY13_14120"
     CDS             complement(1577..2494)
                     /locus_tag="PKY13_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT81126.1"
                     /translation="MNRLCEGRIVVVTGAGRGIGREHALEFARQGAHVVVNDVGAEVD
                     GSGGSIGPASEVVTAIEALGGRAVVNGDDISTDDGAKRLIDTAVEAFGGLDVVVNNAG
                     ILRDRMLVNMTFDEWDAVIRVHLRGTFATSHHAANYWRDRSKAGDDVAGRIINTSSPS
                     GIYGNVGQTNYGAAKAGIASFTIIAAMELARYGVTVNAIAPVAATRMTIPLQAGGAPT
                     VADGEWSPGDPGNIAPLVVWLGSREAAGVTGRVFNVRGDRISVAEGWHAGEAVEAGRR
                     WDPVELGEIIPGLVERAQPNADMSGRLPD"
     gene            2599..>4450
                     /locus_tag="PKY13_14125"
     CDS             2599..>4450
                     /locus_tag="PKY13_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81127.1"
                     /translation="MIGHVHVVPSGRPAGQALASAIRAAKGAAALAPVTAIVPSNFAG
                     LAARRLLATGELRPADADTSAPVGVANVSFLTPFGLAELLAADQLLDRHPLTNPVLGA
                     AIRLTLRDDPGPFARVRDHIATETALAALYAELSNVSEPSLQRLESEGGRAARIAAGF
                     QRSIAARLAEFHDEADMAHAAASRHDLEAALAPFGHLIWYLPAPTTAPIAEFLRAVLH
                     TADATVVVGVTGDLGADAAVWRTCVRAGLSIPDPRPVVETPTASRIISVTDADEEVRA
                     VVREIVTLAEAGMPLDRIGVFHPTPTPYVGILRQQLAAAGIPSNGPSTLTVRDTAAGR
                     TLLAALALPAQRWRRDRVMALVSGAPVRHDGELARPAAWETISREAGVVHDLGDWRRR
                     LEVHRSSLQRHLDQPLPGSTVNIEHTAAAIDETRALASFVEHLADAVTAVETATGWAA
                     KSSAGLALLSQLLGDDRQHGQWPESERAAFERVEDALSRLGALDHLEPRPTAAVFARA
                     LAAELDVTQGRVGRFGDGVLYGPLASAAGLDLDAVFLVGCTEGLCPAARRDDALLPDA
                     ARVLTDGELELRAARIDDQHRQFLVALATAPPEGRVLTIARGDLRGSRRSL"
BASE COUNT          599 a         1670 c         1497 g          684 t
ORIGIN      
        1 agcgccacat ctggtgcccc tcgcagtaca gaaccccgag aatcaggcgt tcttgggcag
       61 gtcggcgaac ggcatcgtct tgtcgttgtt gggctccttg gggaggccca gcacccgctc
      121 gccgatgatg ttgcgctgga tctggtcggt gccgccgtag atgggcggac cctgcgccca
      181 cagggcgatg gaggtgacgg cgccgaggaa gggattgccg gtcgcggcgt cgatcgcagg
      241 gcgctgatcg tcggtgtagg cgtgcagcat tccctgggcg ccgacgatgc gaagacccac
      301 gtcgcggctc agccgcatcg tgtcactcat cgagagcttc gagatgttgg ccatgccggg
      361 aaggtcacga cccgccttct tggccgcctt gacccggagg gcgttgaagc gcccgatctc
      421 gccgagcgtg tggagccgca tgaggtcctg gcggatggtg gcgtcggcga tcgagccgtt
      481 gcccttggcg agatcgatga ggagcctggc agcaccgcca ccactcgacg gacccgctcc
      541 cgacgtcgcc cgcttcttct tctgacgagg cgtgacgaag tcgccgacgc gctggtcgag
      601 gtggccgacg acggtgcccg gcgacgccgc acccgcaccg ccgctgccac cacccgtgcc
      661 gagcccggcc cgctcgttgg cgagcgtggt gttggcgacg gcccatccgt tgttgcgccc
      721 accgatgatc gcggacgacg gcacccgggc gtcggacatg aacacctcgt tgaagagcgc
      781 atgacccgtc atctcacgga gcggacggac ctccatgccc ggctggtgca tgtcgatgga
      841 gaaccaggtg atgccctggt gcttgggcag ctcgggggcc gtacgggcga tgagcatcgc
      901 gaggtcggcc gtgtggccga acgacgtcca caccttctgg ccgttcacga tccactcgtc
      961 accgtcctcg acggcacgcg ccgtgagacc ggcgagatcc gaaccggcgc ccggctcgct
     1021 gaagagctgg caccatgcct tctggccggt gacgatgtcg cgcacgtaca ggtcgatctg
     1081 ttcctgggtg ccgtggctgg cgatcgtcgg tgcggcgagc aggaggccga ggccggcagg
     1141 cgctccgagg gcaccgaagt cgccgatctc gttggcgatc cgcacggcgt cgttgcgcga
     1201 cacccccttg ccgtaggcgt tcgtcggcag gttgggggcg caccagccgg cgaggccgag
     1261 ccgctgccac cagtccgcga cggtcaggtc gggatcccag ttctcctcga gccacgaacg
     1321 cagttcgtcg accagttcgt cgttcgtgac ggcgtcggtg ttctcggatg cggccggagc
     1381 gacatcggtc atcggtaccc ccctgggtga tgctggtgtg atcggcccgg aacccccgtg
     1441 gccgtgaccc ccagatgatc gcgtacaccg tacgactcgt gcccatcctg tgaccgaagt
     1501 cctcccgggc cgggggcgag cgacgacgct ctccaggctc acccgggcgt attcgcccgg
     1561 gtgagcctgg atcgggtcag tcgggcaacc gtccactcat gtcggcgttg ggttgggccc
     1621 gctcgacgag tccggggatg atctcgccca gttcgacggg gtcccagcgg cgaccggcct
     1681 cgaccgcctc gccggcgtgc cacccctcgg ccacgctgat ccggtcgccg cgcacgttga
     1741 acacccgccc ggtgacaccg gccgcctccc ggctccccaa ccagacgacg agcggggcga
     1801 tgttgcccgg gtcaccggga ctccactccc cgtccgcgac ggtcggcgcg ccgcccgcct
     1861 gcagcgggat cgtcatccgg gtggcggcca ccggggcgat ggcgttgacg gtcacgccgt
     1921 agcgggcgag ttccatcgcg gcgatgatcg tgaacgacgc gatgccggcc ttggcggccc
     1981 cgtagttggt ctggccgacg ttgccgtaga tgcccgacgg cgacgacgtg ttgatgatgc
     2041 ggccggcgac gtcgtcgccc gccttggacc gatcgcgcca gtagttggcc gcatggtgcg
     2101 aggtggcgaa cgtgccgcgc aggtggaccc ggatgaccgc gtcccactca tcgaaggtca
     2161 tgttgacgag cattcgatcc cgcaggatgc cggcgttgtt cacaacgacg tcgagcccac
     2221 cgaacgcctc gactgctgta tcgatcaggc gcttggcgcc gtcatcggtc gagatgtcgt
     2281 caccgttgac caccgctcgt ccgccgaggg cctcgatcgc cgtgacgacc tccgacgccg
     2341 gtccgatcga gccgccgctc ccgtccacct cggcaccgac atcgttcacc acgacgtggg
     2401 cgccctggcg ggcgaactcc aaggcatgct cccgaccgat gccgcgcccg gcacccgtca
     2461 ccaccacgat ccggccttca cagagtcgat tcatggcccg attctttcag cgaccgcgcc
     2521 tcggcgctcg gggccggcgc gctcgacccg atcgccgccg ggcttcggtg tcaggaccgt
     2581 cgtttaggtt gaccatcgat gatcggacac gttcacgtcg tgccctcggg gcggcccgcc
     2641 ggccaggcgt tggcgtcggc gatccgcgcc gccaagggcg ccgcagcatt ggcgccggtc
     2701 acggccatcg tgccgtccaa cttcgcaggg cttgcagcca ggcgcctcct ggcgacgggc
     2761 gagctgcggc ccgccgatgc ggacacatcg gcaccggtcg gcgtcgccaa cgtgtcgttc
     2821 ctcacgccgt tcggcctcgc cgagctcctc gccgccgacc agctgctcga ccgccatccc
     2881 ctcaccaatc cggtgctcgg cgccgcgatc cgcctcaccc tgcgcgacga cccgggcccc
     2941 ttcgcccggg tgcgcgatca cattgcgacc gagacggcgc tcgccgcgtt gtacgccgag
     3001 ctcagcaacg tctcggagcc gtcgctgcaa cgtctcgagt ccgaaggcgg acgcgcggcg
     3061 cggatcgccg ccggcttcca acgatcgatc gcggcccggc tggccgagtt ccacgacgag
     3121 gccgacatgg cgcacgccgc cgcgtcccgt cacgacctcg aggccgcgct tgcacccttc
     3181 ggacacctga tctggtacct cccggcgccc accacggcgc cgatcgccga gttcctccgc
     3241 gccgttctcc acaccgccga tgccacggtg gtggtcggcg tcacgggcga cctcggtgcc
     3301 gacgccgccg tgtggcgcac ctgcgttcgc gccgggctct cgatccccga ccctcgtcct
     3361 gttgtcgaga cgccgaccgc gtcgcgcatc atctcggtca ccgacgccga cgaggaggtc
     3421 cgggcggtcg tccgcgagat cgtgacgctg gccgaggcgg ggatgccgct cgaccgcatc
     3481 ggcgtgttcc atcccacacc aactccgtac gtcggcatcc ttcgccagca gctcgcggcg
     3541 gccggcatcc cgtcgaacgg cccgtccaca ctcaccgtgc gcgacacggc ggccggccgc
     3601 acactgctcg ccgccctcgc gctccccgcc cagcgttggc gccgcgaccg ggtcatggcg
     3661 ctggtgagcg gcgcgcccgt gcgccacgac ggcgagctgg cccggcccgc ggcgtgggag
     3721 acgatctcac gcgaggccgg tgtcgtccac gacctcggcg actggcgtcg gcgcctcgag
     3781 gtccaccgct cctccctcca gcgccacctc gaccagccgc tcccgggctc caccgtcaac
     3841 atcgagcaca cggccgctgc catcgacgag acacgagcgc tcgcgtcgtt cgtcgagcac
     3901 ctcgccgacg ccgtcacagc cgtcgagacg gcgacggggt gggccgccaa gtccagcgcc
     3961 ggcctcgccc tcctctcaca gctgctcggg gacgatcggc aacacggcca gtggccagag
     4021 tccgaacggg ctgccttcga acgggtcgag gacgcgctca gtcgtctcgg cgcgctcgac
     4081 cacctcgaac cgcggcccac cgccgccgtc ttcgcccggg cgctggccgc cgagctcgac
     4141 gtcacccagg gccgggtcgg ccgcttcggc gacggggtgc tgtacggacc gctcgcctca
     4201 gccgccggtc tcgacctcga cgccgtgttc ctcgtgggct gcaccgaagg gctgtgcccc
     4261 gcggcccgcc gcgacgacgc gctgctcccc gacgctgccc gcgtcctcac cgacggcgag
     4321 ctcgagctgc gggcggcccg catcgacgac cagcaccgcc agttcctcgt tgccctcgcc
     4381 acggcgccgc ccgagggccg ggtgctcacc atcgcccgcg gcgatctgcg cgggagccga
     4441 cgttcgctcc
//
LOCUS       DAOSOA010000284         4406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19895_length_4406_cov_5.466330, whole genome shotgun sequence.
ACCESSION   DAOSOA010000284 DAOSOA010000000
VERSION     DAOSOA010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19895_length_4406_cov_5.466330"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="PKY13_14130"
     CDS             complement(<1..1396)
                     /locus_tag="PKY13_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81128.1"
                     /translation="MSFRDALPYSVERSRTRRRVGVSAVVFLTAALVADLTGSVPAAA
                     AAPSSVTPSATTIAAPSDYFTNQWNDPMDFNNPEDISLTRLNFNEGSQSMNGGKLRVE
                     GAAQIILTRGDPGAYPTSAVRDPRARTLDGNVYTRVSFRMHSDRTTVGAVGFRTCDGC
                     ADGYRYFDIVPGWHTYDFDMVGTNDLEGWPGGTARFAGTSWGGSIGLVYLAIAFNTPS
                     KPTVTMEDASIYAPANPISVTLAGGSGNVELWQDTNNNPGDDGALSPAVNESANRIGS
                     FPAGSTLSLSSGLFRPNQPVRFYTSQGGSKSAVSGTVTMPADSSPSPVVISPSELGGT
                     DFAAAVRGDAWDFDQATDVLKTFNTNASVHDSALFGFAAGAKNDPVVLFNLGPKQIDA
                     TLYHKVVVKMNYDGPWGLEDAPGGGLVGRFVWSSADGKHEQVSLPIVVKPGPATYVVS
                     FRANPAAAILDPTGK"
     gene            complement(1529..2209)
                     /locus_tag="PKY13_14135"
     CDS             complement(1529..2209)
                     /locus_tag="PKY13_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81129.1"
                     /translation="MPITTPTAPAAPTRIPEPRSAVADRAPTDRSPALDPPVTASTAL
                     PPVLEIVAWTDPSFELNGHDPRSAYVERYWLGLLGPSTTWMLRRFARGLEECPGGFRI
                     DLVETGRALGLGESMARSSTTHRSVLRACQFGAAYRVSQQRLAVRTHLPTLTRRQVAR
                     LPEALQRSHESWARGSVDPEELRRASAAASGLRSVGEALGQVEDQLRRWGYAPAVARE
                     AAKLAFGW"
     gene            complement(2237..3127)
                     /locus_tag="PKY13_14140"
     CDS             complement(2237..3127)
                     /locus_tag="PKY13_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81130.1"
                     /translation="MGATGADVSGAGPARFRRLAGIVAVVVAASGCALDDSGSVAVRR
                     PVDAPAGLEIVFGHCAAGSDVRIGTDRLTDIDVRVESVPGESTTTTTAPLDPDDEPAS
                     PSFGRTQFDRIIWSVHADEPRRLDAVRVGSTPRGFATVIPLEVPLPERLLIVARSDGD
                     SSSDGVAELSVILGNVPADGTFVLDENGTTTLDEFLDRVRTEECGLASTDAGRSSLGS
                     TLTSLLVRLAILSGSLLAVIAAVLVWRRRREEDLDESDEGLDADSDAAADETAVPPLS
                     MGDVPPPIDNGPVVRSDDFP"
     gene            3269..3634
                     /locus_tag="PKY13_14145"
     CDS             3269..3634
                     /locus_tag="PKY13_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81131.1"
                     /translation="MATDDVRTRLRTALTTAMKARDRDVAAALRTVLAALDNAEAVAA
                     PEPPVGADSPIAGALSGLGAGEAPRRELTETDVAMIVHAEIADLRASADDYDRLGRHD
                     RASGLRAGADAVAAAVAPT"
     gene            3673..>4406
                     /locus_tag="PKY13_14150"
     CDS             3673..>4406
                     /locus_tag="PKY13_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOT81132.1"
                     /translation="MTEPDPAVDAAVDAAVDAARRLVAASSRIVVLTGAGISTDSGIP
                     DFRGPNGLWTRSPDAEKLSSIDHYLADPEIRRRAWLGRLDHPAWTSDPNAGHAAIVRL
                     DDLGRLHLLITQNVDGLHHESGLDPDRIVEIHGSIREVECLSCGFRGPMQETLDRVRS
                     GEADPPCLMCGDGILKSATISFGQGLVAADLDRSFDAARRCDLLVAVGTNLSVFPIAN
                     VVPLAARAGAPVVIVNGSPTEMDELAT"
BASE COUNT          698 a         1562 c         1506 g          640 t
ORIGIN      
        1 atttgccagt cggatcgagg atggccgccg cagggttggc ccggaacgac accacatagg
       61 tcgcaggccc gggcttcacg acgatgggga ggctcacctg ctcgtgtttg ccgtccgcgc
      121 tgctccacac gaaccggccg acgagaccgc cgccgggcgc atcctcgagg ccccagggcc
      181 cgtcgtagtt catcttgacg acgaccttgt ggtacagcgt cgcgtcgatc tgcttcgggc
      241 cgaggttgaa caggacgacc gggtcgttct tggctccggc agcgaagccg aacagcgcgc
      301 tgtcgtgcac gctcgcgttc gtgttgaagg tcttgagcac atcggtcgcc tggtcgaagt
      361 cccacgcatc accccggacg gccgcggcga agtcggttcc gcccaactcg ctcgggctga
      421 tgacgacggg actcggagac gagtcggccg gcatcgtcac cgtccccgac acggccgact
      481 tcgaaccacc ctgggaggtg tagaaccgca cgggctggtt cggtcggaag aggcccgagg
      541 agagcgacaa cgtgctcccg gcggggaagg aaccgatgcg gttggccgac tcgttgaccg
      601 caggagacaa cgctccgtca tcccccgggt tgttgttcgt gtcctgccac agctccacgt
      661 tgccgctccc accggccagc gtgacgctga tcgggttggc cggcgcgtag atgctggcgt
      721 cctccatggt cacggtcggc ttgctcggtg tgttgaaggc gatcgccagg tacacgaggc
      781 cgatcgaacc accccaggac gtgccagcga agcgggccgt cccacccggc cagccctcga
      841 ggtcgttggt gccgaccatg tcgaagtcgt aggtgtgcca gccgggcacg atgtcgaagt
      901 agcggtagcc gtccgcgcac ccgtcacagg tgcggaagcc gacggcgccg accgtggtgc
      961 gatcggagtg catccggaac gacacccggg tgtagacgtt gccgtcgagg gtgcgggcgc
     1021 gcgggtcgcg caccgccgac gtcgggtacg cgccgggatc gccgcgggtc aggatgatct
     1081 gcgcggcacc ctcgacccgc agctttccgc cgttcatcga ctgcgatccc tcgttgaagt
     1141 tcagccgcgt gaggctgatg tcctcggggt tgttgaagtc catcgggtcg ttccactgat
     1201 tggtgaagta gtccgagggc gcagcgatgg tggtggccga cggggtcacc gacgaggggg
     1261 ccgcagcggc cgcgggcacc gagccggtca ggtcggcgac gagcgccgcc gtcaggaaca
     1321 cgacggcgct gacaccgacg cgacgacggg tccgtgaacg ttcgacggag tacggcagcg
     1381 cgtctcgaaa cgacatgagg gaggggctcc aggggatcgg caggcgaggg gctctcttca
     1441 gccctacccc tcccatcggc aaggtcgccg aattccatga gtcgatccgg cccgcgttca
     1501 cccgtgcggg tgggtcaccc cccgggaatc accagccgaa cgcgagcttc gccgcctccc
     1561 gcgccaccgc cggggcgtag ccccaccggc gcagctggtc ctcgacctga ccgagagcct
     1621 ccccgaccga ccgtaggccc gacgcggccg ccgacgcacg acggagttct tcagggtcga
     1681 ccgagccccg ggcccacgac tcgtgggagc gctgcaacgc ctcgggaagc cgcgccacct
     1741 ggcggcgcgt caacgtcggc aggtgggtcc gcaccgccaa ccgctgctgc gacacccggt
     1801 acgcggcccc gaactggcag gcccgcaaca cgctccggtg ggtcgtcgaa ctgcgcgcca
     1861 tgctctcccc caggcccagt gcacggccgg tctcgaccag gtcgatccga aacccacccg
     1921 ggcactcctc gaggccacgg gcgaaccgcc tcaacatcca cgtggtactc ggaccgagga
     1981 gacccaacca ataccgctcg acgtacgcgc tgcgcgggtc gtggccgttg agttcgaagg
     2041 acggatcggt ccacgcgacg atctcgagga ccggcggcag ggccgtggac gcggtgaccg
     2101 gtgggtcgag cgcaggcgaa cggtccgtcg gcgcccgatc agcgacggcg gaacggggtt
     2161 cggggatccg cgtgggcgcg gcgggagcgg tgggggtcgt aatgggcatg gggcagtcct
     2221 gacggtgcga tgacgctcag gggaagtcgt cggaccgtac gacgggaccg ttgtcgatgg
     2281 gaggtgggac atcccccatc gacaacggcg gcaccgccgt ctcgtcagcg gcggcatccg
     2341 aatccgcatc cagaccttcg tcagactcgt cgagatcctc ctcacgacgg cgacgccaga
     2401 cgagcacggc ggcgatcacc gcgagcaagc tgcccgacag gatcgcgagt cgaaccagca
     2461 acgacgtcag ggtggatccg agactcgaac gaccggcatc ggtcgaggcg aggccacact
     2521 cttcggtgcg cacccggtcg aggaattcgt ccaaggtggt cgtgccgttc tcgtcgagca
     2581 cgaacgtgcc gtcggccggc acgttgccca ggatcacact cagttccgcg acgccgtcgg
     2641 aggacgagtc gccgtccgac ctcgccacga tcaacaaccg ctcgggcagg ggcacctcga
     2701 gcgggatcac ggtggcgaat ccccgcggcg tcgatccgac ccgcacggcg tcgagccgtc
     2761 gaggctcgtc cgcgtgcacc gaccagatga tgcggtcgaa ttgtgtccgg ccgaagctcg
     2821 ggcttgccgg ttcgtcgtcc ggatcgagcg gggcggtcgt cgtggtcgtc gattcgccgg
     2881 gcacggattc cacccggacg tcgatgtcgg tcagccggtc cgtgccaatg cggacatcgc
     2941 ttccggcagc gcagtggccg aagacgatct cgaggccggc gggcgcgtcg acgggccgtc
     3001 gcacggccac cgagcccgaa tcgtcgaggg cacatcccga tgcggcaacc accactgcga
     3061 cgatgccggc gagtcgacgg aaccgtgccg gtccggctcc gctcacgtcc gctcctgtcg
     3121 cgcccatcgt gagcatcctg gcagtcttcg ccgacgaccc gctcgcgtcg ggcgcgagac
     3181 cgcaacacgg ttccgacccc gaacgtgtag ctccaccgac gaaagaaacc ccgcggcgcg
     3241 accacacctc gctccgtatc ctccaaccat ggcgaccgac gacgtccgga cacgactgag
     3301 gactgcgctg accacagcga tgaaggcgcg cgaccgcgac gtggcggcgg cattgcggac
     3361 cgtgctcgca gcgctcgaca atgccgaggc ggtcgccgcc cccgagcctc cggtcggcgc
     3421 cgactccccg atcgccggtg cgctctccgg gctcggcgcc ggcgaggcac cccgccgcga
     3481 gctgaccgaa accgatgtcg caatgatcgt tcacgcagag atcgccgacc tgcgcgccag
     3541 cgctgacgac tacgaccgcc tcgggcggca cgaccgggcg agcgggctcc gcgctggcgc
     3601 cgatgccgtg gcagcggccg tcgcaccgac gtgagccgct gatcccggcg ctgcgtgggt
     3661 agggtccgcc ccatgaccga gccggaccca gccgtcgacg ccgcagtcga cgccgcggtc
     3721 gacgccgccc gacggctcgt cgcagcgtca tcgcggatcg tggtgctcac cggtgccggc
     3781 atctcgacgg actccggcat ccccgatttc cggggcccaa acggcctctg gacccgctcc
     3841 cccgatgccg agaagttgtc gtcgatcgac cactacctcg ccgaccccga gatccgccgc
     3901 cgggcgtggc tcgggcggct cgaccatccg gcgtggacgt cggaccccaa cgctgggcat
     3961 gcagcgatcg tccgtctcga cgatctcggc cgcctccatc tcctgatcac ccagaacgtc
     4021 gacggcctcc accacgagtc ggggctcgat cccgaccgga tcgtcgagat ccacgggtcg
     4081 atccgtgaag tcgagtgcct ctcgtgcggg ttccggggcc cgatgcagga gacactcgac
     4141 cgcgtccgtt ccggtgaggc cgatccgccg tgcctcatgt gcggcgacgg catcctcaag
     4201 tcggccacca tcagcttcgg ccagggcctc gtcgcagccg atctcgatcg gtcgttcgac
     4261 gccgctcggc gctgcgacct gctcgtcgcg gtcggcacca acctgtcggt gttcccgatc
     4321 gcgaacgtgg ttcccctcgc cgcccgggcg ggcgcacccg ttgtgatcgt caacgggtcc
     4381 ccgacggaga tggacgagct cgccac
//
LOCUS       DAOSOA010000285         4397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_19935_length_4397_cov_7.676877, whole genome shotgun sequence.
ACCESSION   DAOSOA010000285 DAOSOA010000000
VERSION     DAOSOA010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19935_length_4397_cov_7.676877"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="PKY13_14155"
     CDS             <1..1786
                     /locus_tag="PKY13_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOT81133.1"
                     /translation="TEIHGVVNWALEEVGEFLGADRAYILTFDNDARTETMIHEWARP
                     GVEPELGTYDDVSWDLAPAASRRNSDLWVSAVPDVSKLDGEWELDRRFFEASGLRSIL
                     ELPIIIDGRPVGSLGFDWLRQLADWTEDDLTLLGMFASTFAQILGRETAERELVRRAN
                     HDELTGLPNRLGLLTRLRSALDERNRDHVVGAIVLDVDRFQVVNDSLGNAIGDALLRL
                     VGARVRGLVRPTDVVARLGGDEFAVVLTDALDEWSVSQMAERLREGLSEPFHFADRHG
                     MDRTHLLTVSCGLATSDAESVTAEELLRRAGAAMYRAKELGRARQTSFDVELEHRLAE
                     RLDLDQRLRSAQGRGEFEVHFQPEVELATGRVIGAEALLRWRCDGVLRDAVSFIDVVE
                     ETGLIVSVGRWVLDESCRQAALWCRLDGNDDFIMRVNLSPRQLEHPDLIDQVRDVLQR
                     HDLDPRNLCLEITETALMANAEAALGLLNGLDALGVSLAIDDFGTGYSSLSYLKRFPV
                     DILKIDRSFVDGLPHDTEDHAIVTTIITLAAALGMTVTAEGIETEEQADALIALGCDR
                     GQGWRYSKAVPATDFEAMISPAPAHPIG"
     gene            1815..2606
                     /locus_tag="PKY13_14160"
     CDS             1815..2606
                     /locus_tag="PKY13_14160"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81134.1"
                     /translation="MDLRHGSTTATIDLDGGRLASLRPRGIEVLVTEGERPTRWGSFP
                     MVPWCGRLRDGHLEFDGVGYDLPLTSPPHANHGIAHLQPWSAVSAGDATCVIRTELGE
                     PWPFGGHVVQRFELDDAHLTVTVEVHARHRPMPAMAGWHPWFRRDLGVGEPAELVLDL
                     SSASIHAVGEDGIPTGELVPVPPGPWDASFVGLGADPIVRWAGALELTLSSSFDDWVI
                     FTRPEHALCVEPESGAPNDINRVPHVVLPEQPLVGSITFSWVALD"
     gene            complement(2619..3575)
                     /locus_tag="PKY13_14165"
     CDS             complement(2619..3575)
                     /locus_tag="PKY13_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOT81135.1"
                     /translation="MTDHTYRLLTRSDMDGLVCAVLLKDMGILGEIDFHHPKDVQDGK
                     VEVTERDILTNLPYVPGCGLCFDHHASEALRNSGIPTPGYILQPDAKSAARVVYDYYG
                     GKERFPNISDEMMEAVDKADSAGFSMDDILDPQGWELLSFLMDARTGLGRFRDFRISN
                     YALMMDLIDCCRDMSIEEILALPDVAERVDLFRSQHQLFREQIARVAEVHNNLVVLDL
                     RNEETIFAGNRFVVYAMFPQCDISMHVMWGREKQNTVYTVGKSIFDRRNPVEIGELML
                     GYGGGGHHAAGTCQGDNETADERKAELIERLVALSHEGSALV"
     gene            3718..>4397
                     /locus_tag="PKY13_14170"
     CDS             3718..>4397
                     /locus_tag="PKY13_14170"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOT81136.1"
                     /translation="MQTRESTLSGGRGVTLHTVVWEPDDPSTVVADVVLVHGYGEHSR
                     RYVPVAEVLTAAGFRVSALDLRGHGRTVGLRRGDIDDFDTLVDDVSAYVDTVRTDLPL
                     FAYGHSMGGLAVVRLAERDDSRFAGLVITSPALAAAESIPAPLVAVANVVGKLAPGLR
                     TIALEGDAISRDQAVRDDYDRDPYNFRGKLTAGTGRQMNVAIGAALADAGRITSPVLV
                     LHGTADRLT"
BASE COUNT          633 a         1492 c         1526 g          746 t
ORIGIN      
        1 caccgagatc cacggggtcg tgaactgggc gctcgaagag gtcggcgagt tcctcggtgc
       61 cgatcgtgcc tacatcctta cgttcgacaa cgacgcccgt accgagacga tgatccacga
      121 gtgggcgcgc cccggtgtcg agcccgagct cggcacctac gacgacgtgt cgtgggacct
      181 cgcgccggcg gcgagccgac gcaacagcga cctgtgggtc agcgccgtgc cggacgtgtc
      241 gaagctcgac ggcgagtggg aactcgaccg acgcttcttc gaggcgtcgg gactccgttc
      301 gatcctcgag ttgccgatca tcatcgacgg gcgacccgtg ggctcgctcg gtttcgactg
      361 gcttcgacag ctcgccgact ggaccgagga tgacctcacg ctgctcggga tgttcgcgtc
      421 gacgttcgcg cagatcctcg gccgcgagac cgccgaacgg gagctcgttc gtcgcgccaa
      481 ccacgacgag ctgacgggcc ttcccaaccg gctcgggctc ctgacccggc ttcgttcggc
      541 gctcgacgaa cggaaccgcg accacgtcgt cggcgcgatc gtgctcgacg tcgaccggtt
      601 ccaggtcgtc aacgattccc tcggcaatgc aatcggtgac gccctgctcc ggctggtcgg
      661 tgctcgggtc cgtggactgg tgcgcccgac cgacgtcgtc gcccgcctcg gcggcgacga
      721 gttcgccgtg gtgctgaccg acgcactcga cgagtggtcg gtctcgcaga tggccgaacg
      781 gcttcgcgag ggtctgtcgg agcccttcca cttcgccgac cgccacggca tggaccgcac
      841 ccacctcctg acggtcagct gcgggctcgc gacctccgac gccgagtcgg tgaccgccga
      901 ggagctgctg cgccgtgccg gtgccgcgat gtaccgcgcc aaggagctgg gccgggctcg
      961 tcagacgtcg ttcgacgtcg agctcgagca ccgtctcgcc gagcgcctcg atctcgacca
     1021 gcggctccgg tcggcgcaag gccgcggcga gttcgaggtg cacttccaac cggaggtcga
     1081 actcgcgacc ggtcgtgtga tcggcgctga ggccttgctg cggtggcggt gcgacggtgt
     1141 actccgcgac gccgtgtcgt tcatcgacgt cgtcgaggag acgggcctga tcgtgtcggt
     1201 cggtcgttgg gtgctcgacg agtcctgccg gcaggcggcg ctctggtgcc gcctcgacgg
     1261 caacgacgac ttcatcatgc gggtgaatct gtcgccgcgg cagctcgaac accccgacct
     1321 gatcgatcag gtccgagacg tgctgcaacg tcacgacctc gatccgcgga acctgtgcct
     1381 cgagatcacc gagaccgcgc tcatggcgaa cgccgaggcc gcgctcggac tgctcaacgg
     1441 actcgacgcg ctcggtgtga gcctcgcgat cgatgacttc gggacgggtt actcgtcgct
     1501 cagctacctc aagcggttcc ccgtcgacat cctcaagatc gaccggtcgt tcgtcgacgg
     1561 tctgccccac gacaccgagg atcacgccat cgtgacgacg atcatcacgc tcgccgcggc
     1621 gctgggcatg acggtcaccg ccgagggcat cgagaccgag gaacaggccg acgccctgat
     1681 cgctctcggc tgcgatcggg gccagggttg gcggtactcc aaggcggtgc cggccaccga
     1741 cttcgaggcg atgatctcgc cggctccggc gcatcccatc ggctgagcgg ccaacggctg
     1801 gcatgctggc gggcatggac ctgcgccacg gaagcacgac tgcgacgatc gacctcgacg
     1861 gcggccggct cgcctcgctc cggccccggg gcatcgaggt gctcgtgacc gagggggagc
     1921 gcccgacgcg ttgggggtcg ttcccgatgg tgccctggtg cggccggctc cgcgacggtc
     1981 acctcgagtt cgacggcgtc ggttacgacc tgccgttgac cagcccgccg cacgcgaacc
     2041 acggcatcgc tcacctgcag ccgtggtcgg cggtgagcgc aggtgacgcc acctgtgtga
     2101 tccgcaccga gctgggtgag ccgtggccgt tcggcggcca tgtcgtgcaa cgattcgaac
     2161 tggacgacgc ccacctgacg gtgaccgtcg aggtgcacgc ccgccaccgc ccgatgccgg
     2221 cgatggcggg gtggcatccg tggttccggc gtgacctcgg ggtcggtgag ccggccgagc
     2281 tcgtgctcga cctgtcgtcg gcctcgatcc atgcggtggg cgaggacggc atcccgaccg
     2341 gcgagctggt gccggtgccg cccggtccgt gggacgcgtc gttcgtgggc ctcggtgccg
     2401 acccgatcgt ccgctgggcc ggggccctcg aactcacgct ctcgtcgtcg ttcgacgact
     2461 gggtgatctt cacccgcccc gagcatgccc tgtgcgtcga gcccgagagc ggtgcaccca
     2521 acgacatcaa ccgcgtcccg cacgtggtgc tgcccgagca gcccctggtc ggttcgatca
     2581 cgttctcctg ggtcgccctc gactgaccac cgccgcggtc agaccagggc ggacccctcg
     2641 tgggagagcg ccacgaggcg ctcgatgagc tcggctttgc gctcgtcggc cgtctcgttg
     2701 tcgccctggc acgtgccggc ggcgtggtgg ccaccgccgc cgtagcccaa catgagctca
     2761 ccgatctcga cggggttgcg acgatcgaag atcgacttgc cgaccgtgta caccgtgttc
     2821 tgcttctccc ggccccacat cacgtgcatc gagatgtcgc actgggggaa catcgcgtag
     2881 accacgaacc ggttgccggc gaagatcgtc tcctcgttgc gcaggtcgag caccacgagg
     2941 ttgttgtgca cctcggcgac gcgggcgatc tgctcgcgga acagctggtg ctgtgagcgg
     3001 aagaggtcga cgcgttcggc gacgtcgggc agggcgagga tctcctcgat gctcatgtcg
     3061 cggcagcagt cgatgaggtc catcatcaac gcgtagttcg agatgcggaa gtcgcggaag
     3121 cggccgaggc cggtgcgtgc gtccatgagg aacgagagca gttcccagcc ctgggggtcg
     3181 aggatgtcgt ccatcgagaa gccggcggag tcggccttgt cgaccgcctc catcatctcg
     3241 tcgctgatgt tcgggaaccg ctccttgccg ccgtagtagt cgtagacgac ccgggcggcc
     3301 gacttggcgt cgggctgcag gatgtagccg ggcgtgggga tcccggagtt gcggagcgcc
     3361 tccgaggcgt ggtggtcgaa gcagagtcca cagccgggca cgtacggcag gttggtgagg
     3421 atgtcgcgtt cggtcacctc gaccttgcca tcctggacgt ccttgggatg gtggaagtcg
     3481 atctcgccca ggatgcccat gtccttgagc agcacggcac agacgagacc gtccatgtcg
     3541 ctgcgggtca gaagtcggta ggtgtgatcg gtcatggtgg tctccccggg aactggctcg
     3601 gtcgcggcgg tggccggacc catggtcgtt tcatcggcac gggggcctcg atcgttaacc
     3661 gaacggcgtt cggtttgcgc cggattcgcc ggtgcccggc ggaactagcg tggccgcatg
     3721 cagacccggg aatcgacgtt gagcggcggc cgaggggtga ccctccacac agtggtgtgg
     3781 gagcccgacg atccgtcgac ggtggtcgcc gatgttgtgc tcgtccacgg gtacggcgag
     3841 cactcccgtc ggtacgtccc cgtcgccgag gtgttgaccg cagccggctt ccgcgtgtcg
     3901 gccctcgacc tccgtggcca cggacggacc gtggggctgc gtcgcggcga catcgacgac
     3961 ttcgacacgc tcgtcgacga cgtgtcggcc tacgtggaca cggtgcgcac cgacctgccg
     4021 ctgttcgcgt acggccactc gatggggggc ctcgcggtcg tccgcctggc cgaacgtgac
     4081 gactcgcggt tcgccggtct tgtcatcacg agtcccgcgc tggcggcggc cgagtccatt
     4141 ccggcaccgc tcgtcgccgt cgccaacgtg gtgggcaagc tggccccggg cctccgcacg
     4201 atcgcgctcg agggtgacgc gatctcccgc gatcaggccg tgcgcgacga ctacgaccgc
     4261 gatccgtaca acttccgtgg caagttgacc gccggcaccg gtcggcagat gaacgtcgcg
     4321 atcggcgcgg cgttggccga cgccggtcga atcacgtctc ccgtcctggt cctgcacggc
     4381 accgccgatc ggctgac
//
LOCUS       DAOSOA010000286         4350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20215_length_4350_cov_6.140396, whole genome shotgun sequence.
ACCESSION   DAOSOA010000286 DAOSOA010000000
VERSION     DAOSOA010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20215_length_4350_cov_6.140396"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKY13_14175"
     CDS             complement(<1..276)
                     /locus_tag="PKY13_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81137.1"
                     /translation="MKLVFLGTPAIAVTPLRALHAAGHEIDVVVTDPDVRRGRGTETS
                     PSPVPTGLRRPPSDQVPVFGSNRAVRLAQRLGPLNESVDTIVNQLIDA"
     gene            complement(273..875)
                     /gene="def"
                     /locus_tag="PKY13_14180"
     CDS             complement(273..875)
                     /gene="def"
                     /locus_tag="PKY13_14180"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT81138.1"
                     /translation="MSPVTSPYAIRIVGDPVLRQKTPEMTDIDGKMHDLVQGMFEAMY
                     AAPGIGLAAPQVGVKERFFVYDLGDDRGGQQVLINPVITGSDGEWTYEEGCLSVPGQY
                     FEITRPKEIEVTGRDLDGNEVSFEADELLARLVQHELDHLDGVLLLDHLDDDQAKAAK
                     KHVRELQMRKANEAADLEALRSPSLRTEAPKKRFFGLGAK"
     gene            940..1563
                     /locus_tag="PKY13_14185"
     CDS             940..1563
                     /locus_tag="PKY13_14185"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT81139.1"
                     /translation="MPNDQYFTAAPSSASQRREVRLDLDDLSLVLTTDRGVFSMERVD
                     PGTRVLLAEAPRPTPSMLHLADVGCGYGPVALVLAHRAPQATVWAIDTNERARELCAL
                     NAERAGLTNIQVVAPDEVPDDVVFDGIWSNPPIRIGKAQLHDLLTQWLGALARGGTAW
                     LVVQKHLGADSLARWLDEAGWPTTRLASRQAYRVLEVGASGHESELD"
     gene            1538..2071
                     /locus_tag="PKY13_14190"
     CDS             1538..2071
                     /locus_tag="PKY13_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOT81140.1"
                     /translation="MATNLSSTELKRLHREWRRRTPARLSIGLDGVQGPFNVGAIIRS
                     AAAYKSEYLWLTETATGPDNSKVDKTALGTARYLTTNRVADATAIADAARAAGYRVVG
                     VELAAGAVPMHELDLRGDVCLVVGHEDRGLSKAGLAACDAFGFLPQLGSVGSLNVATA
                     ASIAMYEWARQQWTRPS"
     gene            complement(2151..4043)
                     /locus_tag="PKY13_14195"
     CDS             complement(2151..4043)
                     /locus_tag="PKY13_14195"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81141.1"
                     /translation="MGSSPEAGPARLFDVGTPRAQSPSEPPSGRTVRVVCDVASIHRE
                     FDYVIPDRLLSRSGIAASPTHAARGGAGFAGAELLIGAMVRVPLHGRQVAAWIVDVDV
                     ETPEGVELRAVTKVSGLGPPADVIELCRWAAWRWVGRLPTFLGAASPPTMVASLPRPA
                     AAPGPEATLRVGSRVASLFDRPASLIRLPPASSPLEVIRAAAAHGNVLVVAPTFATVK
                     SLAEGMRRAGITTAVHPDGWARGAAGCSVIGTRTAAFAPVAGLGAIVVVDEHDETLQN
                     EGSPTWHARDVAVERARRAGVPVVLVSPTPSLEAIALAGPVTTIDRARERAGWARLEV
                     IDRRDDDIVRSGLYSEALVRALQSDRRVVCVLNRTGRAQMLGCLKCGTLAACERCEAA
                     VRQDDEGVLRCPRCELERPTICDHCAATVFRPIRIGVTKAREQLETLLREPVDEIAGA
                     KATSTATGRRARVVVGTEAALQRVAAADVVAFLEFDQELGAPRYRAVEQAMTLLARAS
                     RLVRGREGSVLVQTRQPDHEVLRAATIGDPSAVSSSEAERRELLQLPPFATVAAIGGE
                     AAPAFVEALAPRLADVPGALLDQRDEGQWLVRADDRRALLDALNAVKRPPGRLRLQVD
                     PARLPT"
     gene            complement(4033..>4350)
                     /locus_tag="PKY13_14200"
     CDS             complement(4033..>4350)
                     /locus_tag="PKY13_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOT81142.1"
                     /translation="WVAKNVVASGAAKRCEVQVAYAIGMARPVSILVETFGTATVDGG
                     KLTDAVKEVFDLRPAAIIRDLDLRRPVFQPTAAYGHFGRSGDGFTWELTNRVDALKSA
                     LGL"
BASE COUNT          631 a         1567 c         1547 g          605 t
ORIGIN      
        1 ggcatcgatc agctggttaa cgatcgtgtc gaccgactcg ttcagcgggc cgagcctttg
       61 cgcaagccgc actgccctgt tcgatccgaa gaccggaacc tggtctgacg gtggccggcg
      121 caagcctgtc ggcaccggcg acgggctggt ctcggtgccc cgcccgcgac ggacgtcggg
      181 gtccgtcacg accacgtcga tctcgtgacc ggccgcgtgg agcgcccgga gcggggtgac
      241 ggcgatcgct ggcgtgccga ggaagacgag cttcacttgg cgccgaggcc gaagaaccgc
      301 ttcttcggcg cttcggtgcg caggctcggg ctgcggagcg cctcgagatc ggccgcctcg
      361 ttcgccttgc gcatctgcag ctcgcggacg tgcttcttgg ccgccttggc ctggtcgtcg
      421 tcgagatggt cgagcagcag cacaccgtcg aggtggtcga gttcgtgctg gaccagacga
      481 gccaacagct cgtcggcctc gaagctgacc tcgttgccgt cgaggtcgcg cccggtgacc
      541 tcgatctcct tgggccgggt gatctcgaag tactggccgg ggaccgacaa gcagccctcc
      601 tcgtaggtcc attcgccgtc ggagccggtg atgaccgggt tgatcagcac ctgctggcca
      661 ccgcggtcgt cgccgaggtc gtagacgaag aagcgttcct tgacgcccac ctggggcgcg
      721 gccagaccga tgccgggagc ggcgtacatg gcctcgaaca tgccctgcac gaggtcgtgc
      781 atcttgccgt cgatgtcggt catctccggc gtcttctggc gcagcacggg gtcaccgacg
      841 atgcggatcg cgtacgggga ggtgaccggg gacatgggtg tcagtgtact tcgcggcacc
      901 ccagcgggga ccaccggcac cagccgctac ggtgccggtg tgccgaacga ccagtacttc
      961 acggccgcgc cgtcgtccgc gtcgcagcgc cgcgaggtcc gcctcgacct cgacgacctg
     1021 tcgctcgtgt tgacgaccga tcgtggggtg ttctccatgg aacgggtcga tcccggcacc
     1081 cgggtcctgt tggcggaggc tccccgcccc acgccctcga tgctccacct cgcggacgtg
     1141 gggtgcggct acgggccggt ggcgctggtc ctcgcccatc gagcgcccca ggcgacggtg
     1201 tgggcgatcg acaccaacga gcgagctcgg gagctgtgcg cgctcaacgc cgagcgggcc
     1261 ggcctcacga acatccaggt cgtcgcgccc gacgaggtgc ccgatgacgt cgtgttcgac
     1321 ggcatctggt cgaacccgcc gatccgcatc ggcaaggccc agctgcacga cctgctcacc
     1381 cagtggctgg gcgcactcgc ccgcggtggc acggcgtggc tggtggtgca gaagcacttg
     1441 ggtgccgact cgctcgcccg ttggctcgac gaggccggct ggcccacgac acgcctggcg
     1501 tcgcgccagg cctaccgagt cctggaggtg ggggccagtg gccacgaatc tgagctcgac
     1561 tgagctgaag cggttgcacc gggaatggcg ccgccgcacc cccgcccgtc tgtcgatcgg
     1621 gctcgacggg gtgcaggggc cgttcaacgt cggcgccatc atccgctcgg cggccgccta
     1681 caagtccgag tacctgtggc tcaccgagac cgcgaccggc cccgacaaca gcaaggtcga
     1741 caagaccgcg ctgggaacgg cgcggtacct gacgacgaat cgggtcgccg acgccaccgc
     1801 gatcgccgat gccgctcgcg ccgccgggta ccgcgtggtg ggcgtcgagc tcgccgccgg
     1861 tgcggtaccg atgcacgagc tcgacctccg cggcgacgtc tgcctcgtcg tcggccacga
     1921 ggaccgaggc ctctccaagg ccggcctcgc ggcgtgcgac gcgttcgggt tcctgcccca
     1981 gctcggcagc gtcggttcgc tcaatgtcgc gacggccgcc tcgatcgcga tgtacgagtg
     2041 ggctcgccaa cagtggacac ggccaagctg acctgacctc gctcgttctg tcctgcagaa
     2101 cggcccccac agaggcggtt ctgcaggaca gaaccggtgc gaacctgcgc tcaggttggc
     2161 aggcgggccg ggtcgacctg gagtcggagg cggccgggcg ggcgcttcac ggcgttgagg
     2221 gcgtcgagca gggcccgacg gtcgtcggct cgcaccaacc actggccctc gtcgcgctgg
     2281 tcgagcagcg ctccgggcac gtcggcgagc cgaggtgcga gcgcctcgac gaacgccggt
     2341 gcggcctcgc caccgatcgc ggccaccgtc gcgaacggcg gaagctgcaa gagctcacgg
     2401 cgctcggcct ccgagctcga gacggcgctc gggtcgccga tggtcgccgc ccgcagcacc
     2461 tcgtggtcgg gctgacgcgt ctgcaccagc acggagccct cacggccgcg cacgaggcgg
     2521 gatgcccggg ccagcagggt catcgcctgc tcgaccgccc ggtagcgggg cgcccccagc
     2581 tcctggtcga actcgaggaa cgccaccacg tcggccgctg caacccgctg cagcgccgcc
     2641 tccgtgccga cgaccacgcg ggctcggcga cccgtcgccg tcgaggtcgc cttggccccg
     2701 gcgatctcgt cgaccggttc tcgcaggagt gtctcgagct gctcccgcgc cttcgtgacg
     2761 ccgatgcgga tcgggcggaa cacggtcgcc gcgcagtggt cgcagatcgt cggtcgttcc
     2821 aactcgcacc ggggacaccg caacacgccc tcgtcgtcct ggcgcacggc cgcctcgcag
     2881 cgttcgcacg ccgcgagcgt gccgcacttg aggcaaccga gcatctgggc gcggccggtc
     2941 cggttgagca cacacacgac ccggcgatcg gactggagcg cccgcaccag cgcctcggag
     3001 tacaagcccg agcggacgat gtcgtcgtct cgacggtcga tcacctcgag ccgagcccaa
     3061 ccggcgcgct cgcgggcccg atcgatcgtc gtcacgggcc cggcgagggc gatcgcctcc
     3121 aggctcgggg tcggcgagac gagcactacc ggcacccccg cccgccgggc acgctcgacc
     3181 gcgacgtcgc gggcgtgcca cgtcggcgaa ccctcgttct gcagcgtctc gtcgtgttcg
     3241 tcgacgacga cgatcgcccc caaacctgcg accggggcga acgcggccgt gcgtgtgccg
     3301 atcaccgagc atccggcggc accgcgggcc caaccgtcgg ggtgcaccgc cgtcgtgatg
     3361 ccggcacgtc gcatcccctc ggccagcgac ttcaccgtgg cgaacgtcgg cgcgaccacg
     3421 agcacgttgc cgtgggcggc ggccgcgcgg atcacctcga gcggcgacga cgccggcgga
     3481 aggcggatca gcgatgccgg gcggtcgaac aacgaggcga cccgggagcc gacccgcagc
     3541 gtggcctctg gtccgggcgc cgcggccggt cggggcaggc tcgccaccat cgtcgggggc
     3601 gacgctgcgc cgaggaaggt gggcaaccgg ccgacccacc gccacgccgc ccaacggcac
     3661 agctcgatga cgtcggcggg tggccccaag cccgacacct tggtcacggc acggagctcg
     3721 acgccctcgg gtgtctcgac gtcgacatcg acgatccagg cggcgacctg tcgcccgtgc
     3781 aacggcacgc gcaccatggc cccgatgagc aactcagcgc cggcgaagcc cgctccgcca
     3841 cgggcggcgt gggtgggtga cgcggcgatc cccgaccggc tgagcagccg atcggggatc
     3901 acatagtcga actcccggtg tatcgaggcg acgtcacaga cgacgcgcac cgttcggccc
     3961 gacggcggct ccgagggcga ctgcgcccgg ggcgtgccga cgtcgaagag ccgggcggga
     4021 ccggcctcgg ggctagagcc caagcgccga cttgagggcg tcgacccggt tggtcagctc
     4081 ccaggtgaag ccgtcgcccg aacgaccgaa gtggccgtag gcggcggtcg gctggaacac
     4141 cggccggcga aggtcgaggt cgcggatgat ggcggcgggg cggaggtcga agacctcctt
     4201 gaccgcgtcg gtcagcttgc cgccatcgac ggtggcggtg ccgaacgtct cgacgaggat
     4261 cgacacgggg cgggccatgc cgatggcgta cgccacctgc acctcgcagc gcttcgcagc
     4321 gcccgacgcc accacgttct tcgcgaccca
//
LOCUS       DAOSOA010000287         4336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20317_length_4336_cov_6.825508, whole genome shotgun sequence.
ACCESSION   DAOSOA010000287 DAOSOA010000000
VERSION     DAOSOA010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20317_length_4336_cov_6.825508"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKY13_14205"
     CDS             complement(<1..463)
                     /locus_tag="PKY13_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT81143.1"
                     /translation="MHAVILAGGRGVRLRPYTTAFPKPLVPIGDEMAILEVVMRQLQG
                     YGFTEVTIAIGHLGQLIRAYCGDGSQWGLRITYWNEDEPLGTMGPIVANLDQCPEHFL
                     VMNGDVLTDLDYGALLRGHIESGAPITVSTYDRTVKIDFGVLEVGGEHNDRI"
     gene            complement(506..1015)
                     /locus_tag="PKY13_14210"
     CDS             complement(506..1015)
                     /locus_tag="PKY13_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81144.1"
                     /translation="MSHHRGSGHTFIVSTSLGTAQFDTARLAKVRLKAVMAEVKRIRR
                     SDRRVRGAKLRPVSSVEGGKYLLELTIAAPSGYEAFEIGSAVVRASIHTAGGSTAGWE
                     TNVVPQLAVGAMRGRVRPTTPSSWAEVTATAARHAATLPVLPSFDPALAPVAPASPRL
                     APAGYIDLR"
     gene            1207..2013
                     /gene="yaaA"
                     /locus_tag="PKY13_14215"
     CDS             1207..2013
                     /gene="yaaA"
                     /locus_tag="PKY13_14215"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOT81145.1"
                     /translation="MPAPIVLLPPSEGKADGGTGPPWTPGSISFPELDASRRKVAAAL
                     KRTAKQNVAVNAKLLGVKGDALAAARAANRDVLDSPTLPAIERYTGVLYDAFDHATLP
                     AAQRRRAAAQLVIFSGLWGVVTPTDLLPDYKLKMGASLAPMGRLATFWRPQISAALAP
                     FVEGRTVWDLLPNEHAAAWQPEAGSLRRRISVKFLDEVPDGGGSKLVAVSHWNKLLKG
                     ALVRHVLATQLDDPEGLVEFDHPMGYRYEPGLTTETADGVTHVSLVARRA"
     gene            2037..2867
                     /locus_tag="PKY13_14220"
     CDS             2037..2867
                     /locus_tag="PKY13_14220"
                     /inference="COORDINATES: protein motif:HMM:NF022740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HOT81146.1"
                     /translation="MSSPKYQGGILSSLDDIDNGPSKRKEYPRVPANPGLEVTHQASG
                     VIGAIVEWTDAAITIRDDSGRDRRMRNIPGSFLLHGKPCTLVRPNVAATSGPRITASG
                     SVADPTATAKIAKASRILVEGIHDAELVEKVWGDDLRSEGIVVQSIDGADDLTEVVAA
                     FGPRPNRRLGVLLDHLVADTKESRIAARIDHPDVLIRGHVFIDIWAAVNPKLIGLDAW
                     PDIPKGLPWKDGICAAVGIAESWQFWKMLLGEVTSYKDLNPSLVGAVEELIDFVCDAG
                     "
     gene            complement(2795..3376)
                     /locus_tag="PKY13_14225"
     CDS             complement(2795..3376)
                     /locus_tag="PKY13_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81147.1"
                     /translation="MTSPLWLSHHHEHQADRCIRVGDAHVCRRCAVLYPGMLVVAAIQ
                     LAGWIPADLGIVLMWVMPLGVVGEWIAEHLAGVAYSAGRQIVTTALASVSFGVAFGRH
                     VQHPFEAAATAPAVTFTVLCLVVWFIGARRLDPASTEWEAEFESAEEQRLDALLALLE
                     DNGLEAGRPDQPASQTKSMSSSTAPTNDGLRSL"
     gene            complement(3373..>4336)
                     /locus_tag="PKY13_14230"
     CDS             complement(3373..>4336)
                     /locus_tag="PKY13_14230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT81148.1"
                     /translation="WWLAEVAIGDPRIVLVFDDDDLVGGLALQRSTKAGVEVLQFLGT
                     GPLEPDHLDLVAAPARVADVVAAIRAWLAAGDRLVDLVGARPAAWVLDAVPGRGEVTD
                     LEVAPYVALPDDRAEYLATRQGRMRSTITRTAKRLAKAGVTFRVVGGPDTEPADVDRS
                     LSALHELHDGRWGDESGFLASWDSFAAAARSGAAAGEVRFHELVSADGEIVAIETDFV
                     VGGRMSFYQAGRLTEHELRGSGSVLRYDVIGAAIEAGCTEFDLLRGGEDYKTDWADQR
                     RGLIRIRRGTGPRSQALVAVARLNAFVQHRRSLRVATEVPTGDT"
BASE COUNT          709 a         1556 c         1450 g          621 t
ORIGIN      
        1 cgatgcgatc gttgtgttcg ccgccgacct cgagcacgcc gaagtcgatc ttcaccgtgc
       61 ggtcgtaggt ggagacggtg atgggcgcac ccgactcgat gtggccgcgc agcaaggcgc
      121 cgtagtcgag gtcagtgagg acgtcgccgt tcatcacgag gaagtgctcg gggcactggt
      181 cgaggttggc gacgatcgga cccatggtgc cgagcggctc gtcctcgttc cagtacgtga
      241 tgcgcaggcc ccactgcgag ccgtcaccgc agtaagcacg gatgagctgg ccgagatgac
      301 cgatggcgat cgtcacctcg gtgaagccgt agccctgcag ctgccgcatc acgacctcga
      361 ggatcgccat ctcgtcgccg atcggcacga ggggcttggg gaaggcggtc gtgtacggac
      421 ggaggcgtac tccccggcct ccggccaaga tcacggcgtg cataggtagt cagtctgcgg
      481 aacggctggt gatctgtcga gtcgatcagc gcaagtcgat gtagccggcc ggcgcgagcc
      541 tcggcgaggc cggcgcgacc ggcgcgagcg ccgggtcgaa tgacggcagc acgggaaggg
      601 tggcagcgtg tcgggcggcg gtcgcggtga cctccgccca ggacgacggt gtggtcggac
      661 ggacccgccc ccgcatggcg cccacggcca gttggggcac cacattggtc tcccacccgg
      721 cggtcgagcc gccggcggtg tggatcgagg cccgcacgac ggcggagccg atctcgaagg
      781 cctcgtaccc cgacggggcg gcgatcgtca gttcgagcag gtacttgccc ccctcgacgc
      841 tcgacaccgg acgcagcttg gcgccccgca cccggcgatc ggagcggcgg attcgtttca
      901 cctcggccat gaccgccttc aatcggacct tggccagacg cgccgtgtcg aactgcgccg
      961 tgcccagact cgtcgagacg atgaaggtgt gcccgctccc gcgatggtga ctcacaccca
     1021 ggtgttcggc gcgccgggtg gaacacctga gcagtttcac catgtcggcc gcgatcgcgg
     1081 ggacatgagg ggatcgggca ggattttctg tgccgacccc acggcaccct cgaatgtcgc
     1141 cggagcatcg gcacgaccct gctcgacgtc ggctgactgc ccgaactgcc tctacgctcg
     1201 tggcccgtgc ccgccccgat cgtcctgctg ccaccgtcgg agggcaaggc cgacggcgga
     1261 accggcccgc cctggacgcc cggttccatc tcgttccccg aactcgacgc gtctcgtcgc
     1321 aaggtcgcgg ctgcactcaa gcgaacggcc aagcagaacg tcgccgtcaa cgccaagctg
     1381 ctcggggtga agggcgacgc actcgccgcg gcccgggcgg ccaaccgcga cgtgctcgac
     1441 tcgccgacgc tgccggcgat cgagcgctac acgggtgtgc tctacgacgc gttcgaccac
     1501 gccacgctgc cggcggccca gcgacgtcgc gccgcggccc agctggtgat cttctccggc
     1561 ttgtggggcg tcgtgacccc gaccgacctg ctccccgact acaagctcaa gatgggcgcc
     1621 tcgttggccc cgatgggtcg gctcgcaacc ttctggcgcc cacagatcag cgccgcgctg
     1681 gcacctttcg tcgagggtcg caccgtgtgg gacctgctgc ccaacgagca cgccgccgcg
     1741 tggcaaccgg aggcggggag tctccgacgc cggatctcgg tgaagttcct cgacgaggtt
     1801 cccgacgggg gcggcagcaa gttggtcgcc gtgtcgcact ggaacaagct cctcaaaggg
     1861 gcgctcgtcc gccacgtcct ggcgacccag ctcgacgatc ccgagggcct cgtcgagttc
     1921 gatcacccca tgggctatcg ctacgagccg ggcctcacga ccgagaccgc ggacggagtc
     1981 acccatgtgt cgctggtggc ccggcgcgcc tgatcgccgc cactaccgtt cggcggatga
     2041 gttctccgaa gtaccagggc ggcatcctct ccagcctcga cgacatcgac aacggtccgt
     2101 cgaagcgcaa ggaatacccc cgggttccgg ccaaccccgg cctcgaggtc acccatcagg
     2161 ccagcggcgt catcggtgcg atcgtcgagt ggaccgacgc cgcgatcacg atccgggacg
     2221 acagcggccg cgaccgccgc atgcgcaaca tcccgggctc gttcctgctc cacggcaagc
     2281 cgtgcaccct cgtccgcccc aacgtggcgg cgacgtcggg cccgcgcatc accgcgtcgg
     2341 gctcggtcgc cgacccgact gccacggcca agatcgccaa ggcgagccgc attctcgtcg
     2401 agggcatcca cgacgccgag ctcgtcgaga aggtgtgggg cgacgacctc cggtccgagg
     2461 gcatcgtggt ccagtcgatc gacggtgccg acgacctgac cgaggtggtt gccgccttcg
     2521 gcccccgacc caaccgtcgt ctcggggtgc tgctcgacca cctcgtcgcc gacaccaagg
     2581 aatcacgcat cgcggcgagg atcgaccacc ccgacgtgtt gatccgcggc cacgtcttca
     2641 tcgacatctg ggccgcagtg aatcccaagc tcatcgggct cgacgcctgg cccgacatcc
     2701 ccaaggggct gccgtggaag gacggcatct gcgccgccgt cggcatcgcc gagtcgtggc
     2761 agttctggaa gatgctcctc ggcgaggtga cgagctacaa ggacctcaac ccgtcgttgg
     2821 tgggagcagt cgaagaactc atcgacttcg tctgcgacgc cggctgatcg ggccggccgg
     2881 cctcgaggcc gttgtcctcg aggagcgcca ggagcgcgtc gaggcgttgt tcctcggccg
     2941 actcgaactc ggcctcccac tcggtcgatg ccgggtcgag ccgccgggcg ccgatgaacc
     3001 acacgacgag gcacaggacg gtgaacgtca ccgccggcgc cgtcgctgcc gcctcgaacg
     3061 gatgctggac gtgccgtccg aacgcgaccc cgaacgagac cgacgccaac gcggtggtca
     3121 cgatctgccg acccgctgaa tacgccacgc cggccagatg ctcggcgatc cactcgccga
     3181 cgactccgag cggcatcacc cacatgagga cgatcccgag gtccgcgggg atccatccgg
     3241 cgagttggat tgcggccacg acgagcatgc ccggatagag aacggcgcaa cggcggcaca
     3301 cgtgagcgtc accgacacgg atgcaccggt cggcctgatg ctcgtggtgg tgcgacaacc
     3361 acagcgggga cgtcatgtgt cccctgtcgg cacctccgtg gcgaccctga gcgaacggcg
     3421 atgctggacg aaggcgttca ggcgggcgac ggcgacgagc gcctgggacc gtggcccggt
     3481 gccacggcga atgcggatga gcccgcggcg ctgatcggcc cagtcggtct tgtagtcctc
     3541 accaccgcgc agcaggtcga actcggtgca accggcctcg atcgcagcgc cgatcacgtc
     3601 gtagcggagc accgaccccg aaccccggag ctcgtgctcg gtcagccggc ccgcttggta
     3661 gaagctcatg cgtcccccga cgacgaagtc ggtctcgatc gccacgatct caccgtccgc
     3721 cgacaccagc tcgtggaacc ggacctcgcc cgcggctgcc cccgagcgcg ccgcggcggc
     3781 gaaggaatcc cagctcgcca ggaacccgga ttcgtcaccc caccggccgt cgtgcagttc
     3841 gtggagcgcg ctgagggacc ggtcgacgtc ggccggctcg gtgtcggggc caccgacgac
     3901 acggaacgtc acgccggcct tcgccaggcg cttcgccgtc cgcgtgatcg tgctgcgcat
     3961 ccgtccctga cgagtggcga ggtactcagc ccgatcatcg ggaagggcca cgtagggcgc
     4021 cacctcgaga tcggtcacct cgcctcggcc cggcaccgcg tcgagtaccc acgccgccgg
     4081 ccgagccccg accaggtcga ccagtcggtc acccgccgcc agccacgcac ggatcgcggc
     4141 cacgacatcg gccacgcggg ccggcgcggc gacgagatcg aggtgatcgg gttcgagtgg
     4201 accggtaccc aggaactgca gcacctccac acccgccttc gtcgagcgct gcagcgcgag
     4261 accgccgacg aggtcgtcat cgtcgaacac cagaacgatc cgagggtcac cgatcgcgac
     4321 ctcagccaac caccac
//
LOCUS       DAOSOA010000288         4329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20357_length_4329_cov_6.333879, whole genome shotgun sequence.
ACCESSION   DAOSOA010000288 DAOSOA010000000
VERSION     DAOSOA010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20357_length_4329_cov_6.333879"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="PKY13_14235"
     CDS             complement(<1..1669)
                     /locus_tag="PKY13_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT81149.1"
                     /translation="MSPQGRRILVVDDEAPIRNLLRRLLTRNGYDVDTVADARTARLE
                     LTSNTYDAVLCDVSLGGESGISLAKWIVAQFPDLPVLMVTGGNRHNARLLRDAGIDGL
                     ILKPFEPAEIAVNVERAIRRSARRRAEVSTLSEALSETTDLLDRTSIELVETQAELMR
                     HEQVWEALVTSAGIGVIVVSTTGQLEFANPRARLVLGLDAVLAEQTDRGVDDTDAWLD
                     VFHPADRAAMAELLTTSGPERSTAQVRTAGGDDTTRHLIVRAASIDVADGAGRRGVVL
                     SIEEITELVATQDRLERRLTTDSTTGLPNRTSLAVHLGTALRATGGSVGSASAGGAVA
                     VNVAAIREVASVDGELEFERIMASIAARLQHAVGPGEVLGRSGPSTFSIVVAGESRSQ
                     LLTSLPSRIMRALIDPLAVGDRRVELEPRIGVALAETGATAESILRDATLASEHADAR
                     GGVRVFNESLRAEAFTRLALEQALREAVSRRQLTLEYQPIVSTVERRLVGMEALSRWH
                     HPGLGVVSPGVFVPIAEQLGLINELGNHVLGAAVAQIGAWNDEFRPGD"
     gene            complement(1788..4121)
                     /locus_tag="PKY13_14240"
     CDS             complement(1788..4121)
                     /locus_tag="PKY13_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT81150.1"
                     /translation="MVLSLVDTGSDFTLAIGHLLDQEGILWTAVPQAPTAPGALPGPV
                     DSGVFVVPLHGGLTAADRAALRDRFEKSTTHLRVFGILDRAVDGDGDLDDDLDAIIDE
                     FVDEVGAVDSPLTVRRLRRFVDHHLDELRADAVKMFLDSTADGYWIWNIADGLVEWSR
                     RTYQILGRTPEDHPMTFEAFIDIVHPDDRHSVEIALQEHFVHGVAYRNVPMRLQTEYG
                     GYRNLVAAGQVVRSPSGDPLLMVGAVTDRTAQLNAEQQAEEAQAKYVRLFQTMNDAAV
                     LADPLTGRIVDVNEPAERLWVRPRSELVGAPQSILHPPDLEEDAEAREVFRRHIRALQ
                     EGNRATIQMPILRSDGVRVPVEISSSIFEIDGTQYILGLFRDISERLQTEQRLRERDT
                     QLQLASRLAAMGSLAAGVGHEINNPLAYIMGNLHYVRDALETVDGVCPDLISAIDDAI
                     DGSVRVRDIVTDLKTLTRGDESDESCDPVAVCEIAVRMANNELRHRAVVGLSADQCDR
                     VAMSSSRLSQVAVNLLTNAAHAFENSDLGVNRVEIGLRTVGDVVHLTVKDNGSGISPE
                     VLARVFEPFFTTKEVIGTGLGLSISRQLVEDAGGDIDIDTEVGWGTVVTVRIPLAADT
                     PTPPDDLPAHDADAAPAAVDPADLPARPSVVIVDDDRKVADSLARTLGGDFAVTTFTD
                     ASEALDAVSSSDRGVDAIVCDLMMPGVHGAEFHARLVAADPAQASRVVFVTGGAVTST
                     AAAFERAMSDAGRLVYKPIDPDGLRAAIDRVAPVTAH"
BASE COUNT          639 a         1386 c         1597 g          707 t
ORIGIN      
        1 agtcgccggg tcggaactcg tcgttccacg cgccgatctg cgccacggcg gcaccgagga
       61 cgtggttgcc gagctcgttg atgagcccga gctgttcggc gatcggcacg aacaccccgg
      121 gcgacaccac accgagcccg ggatggtgcc accggctgag cgcctccatg ccgacgaggc
      181 gccgctcgac cgtgctcacg atcggctggt actcgagggt gagctgacgg cggctcacgg
      241 cctcccggag cgcttgctcg agtgcgagac gggtgaacgc ctcggcgcgg agcgattcgt
      301 tgaagacccg gaccccgccg cgggcgtcgg cgtgctcgga cgcgagcgtc gcgtcgcgca
      361 ggatcgactc ggcggtggcg ccggtctccg cgagggcgac gccgatcctc ggttcgagct
      421 cgacccgccg gtctccgacg gcgagcgggt cgatcagggc gcgcatgatc cgcgacggca
      481 gcgacgtcag gagttgggac cgactctcgc cggcgacgac gatcgagaac gtcgatggcc
      541 cgctccggcc gagcacctcg cccgggccga cggcgtgctg gaggcgggcg gcgatcgacg
      601 ccatgatccg ttcgaactcc agctcgccgt cgacgctggc gacctcacgg atcgccgcga
      661 cgttgaccgc aacggcgccg ccggcggagg cgctgccgac gctgcctccc gtggcccgga
      721 gcgccgtgcc gaggtggacg gcgagcgagg tccggttcgg gaggcccgtc gtcgagtcgg
      781 tcgtgagccg tcgctccagg cggtcctggg tggccaccag ttcggtgatc tcctcgatgc
      841 tgaggaccac cccgcggcgc ccggccccgt cggcgacgtc gatggaggcg gcgcgcacga
      901 tgaggtggcg tgtcgtgtcg tcgccgccgg cggtgcgcac ctgggcggtc gaccgctcgg
      961 gtccggacgt ggtgaggagt tccgccatcg ctgcccggtc agcgggatgg aagacgtcca
     1021 gccaggcatc ggtgtcgtcg acgcctcggt cggtttgctc ggccagcact gcgtcgagcc
     1081 ccaggacgag ccgggcccgt ggattggcga actcgagctg gccggtggtc gacacgacga
     1141 tcacgccgat tccggccgac gtcacgagcg cctcccacac ctgctcgtgg cgcatgagct
     1201 cggcttgggt ctcgaccagc tcgatcgatg ttctgtcgag cagatcggtg gtctccgaca
     1261 gcgcctcgga aagggtcgat acctcagcgc ggcgccgggc acttcgccga atggcgcgtt
     1321 cgacattgac ggcgatctct gccggctcga acggtttgag gatcaacccg tcgatgccgg
     1381 catcgcgtag caggcgggcg ttgtgtcggt tgccgcccgt gaccatcagg accgggaggt
     1441 cgggaaattg ggcgacgatc cacttggcaa gcgagatgcc gctctcgcca ccgagcgaca
     1501 cgtcacagag gacggcgtcg tacgtgttgg acgtgagctc gagccgtgct gtccgggcgt
     1561 cggcgacggt gtcgacgtcg tagccatttc gggtcaggag gcgacggagc aggttcctga
     1621 tcggcgcctc gtcgtccacg acgagaatgc gacgtccttg cgggctcata cgatcaattc
     1681 ggcgttgtgg ttcattcgct gaatcccgtc ctcgacattg ggtatgtttg cccaacgcct
     1741 cgtttccatg gccaatgatc tccgtcgaag ctccggctag gaaccgttca gtgcgcggtg
     1801 acgggagcga cgcggtcgat cgcagcccgc agaccatcgg gatcgatcgg cttgtagacg
     1861 agtcgaccgg catcggacat cgcccgctcg aacgcggcgg cggtcgatgt gacggcgccg
     1921 ccggtgacga agacgacgcg gctggcctga gcgggatcgg cggcgacgag gcgggcgtgg
     1981 aactcggctc cgtgcacccc gggcatcatc aggtcacaga cgatggcgtc gacaccgcgg
     2041 tcggaggacg agacggcgtc gagcgcctcg ctcgcatcgg tgaaggtcgt gaccgcgaag
     2101 tcgccgccga gggtgcgggc cagggaatcc gccaccttcc ggtcgtcgtc gacgatcacc
     2161 accgacggtc gcgccgggag gtcggcggga tcgaccgcgg cgggggcggc gtcggcgtcg
     2221 tgtgcgggga gatcgtcggg tggcgtcggg gtgtcggctg cgagtgggat ccggacggtc
     2281 accaccgttc cccagccgac ctcggtatcg atgtcgatgt cgcccccggc gtcctcgacc
     2341 agttgtcgtg aaatcgatag tccgaggccg gtgccgatga cctccttcgt tgtgaagaag
     2401 ggttcgaaga cccgcgccaa cacctcgggc gagatgccgc tgccgttgtc cttcacggtc
     2461 aggtgcacga cgtccccgac ggtcctgagg ccgatctcga cgcgattgac gccgaggtcg
     2521 gagttctcga acgcgtgggc ggcgttcgtc agcaggttca cggcgacctg gctgagccgc
     2581 gaactcgaca tcgccacgcg gtcgcactgg tcggcggaca gcccgacgac ggcgcggtgg
     2641 cggagttcgt tgttggccat tcgcactgcg atctcacaca ccgcgacggg gtcgcacgac
     2701 tcgtcgctct cgtcacctcg ggtgagcgtc ttgagatcgg tgacgatgtc acggactcga
     2761 accgaaccgt cgatggcgtc gtcgatggcc gagatcaggt cggggcacac accgtcgacc
     2821 gtctcgagtg cgtcgcggac gtagtggagg ttgcccatga tgtacgcgag cgggttgttg
     2881 atctcgtgac cgacgccggc ggcgaggctg cccatcgctg cgagccgtga tgcgagctgc
     2941 agctgggtgt cgcgctctcg gagacgctgc tcggtctgga gccgctcgga gatgtcgcgg
     3001 aacaggccga ggatgtattg ggtgccgtcg atctcgaaga tcgaggaact gatctcgacg
     3061 gggacccgga cgccgtcgga gcgcaggatc ggcatctgga tcgtggctcg attcccttcc
     3121 tgcagtgctc ggatgtgtcg ccggaacacc tcccgcgcct cggcatcctc ctcgaggtcg
     3181 ggtggatgca ggatcgactg tggtgcgccg acgagctcgg atcgcgggcg gacccacagg
     3241 cgctctgccg gctcgttgac atcgacgatg cgtccggtca ggggatcggc cagcaccgcg
     3301 gcgtcgttca tcgtctggaa gagacggacg tacttcgcct gcgcctcctc ggcctgctgc
     3361 tcggcattca gctgggccgt ccggtcggtg accgcgccga ccatcagcaa cgggtcgccg
     3421 gagggcgacc ggacgacctg accggcggcg acgagattgc ggtagccgcc gtactcggtc
     3481 tgcagccgca tcggcacgtt ccggtaggcc acgccgtgga cgaaatgctc ctggagcgcg
     3541 atctcgaccg agtgccggtc gtcggggtgg acgatgtcga tgaacgcctc gaacgtcatc
     3601 ggatggtcct cgggcgtgcg gcccaggatc tggtacgtcc ggcgcgacca ttcgacgagt
     3661 ccgtcggcga tgttccagat ccagtacccg tccgccgtcg agtcgaggaa catcttgacg
     3721 gcgtcggcgc gcagctcgtc gaggtggtgg tcgacgaacc gacgaagtcg ccgcacggtc
     3781 aggggcgagt cgacggctcc gacctcgtcg acgaactcgt cgatgatggc gtcgaggtcg
     3841 tcatcgaggt cgccgtcgcc gtcgacggcc cgatcgagga tgccgaacac ccggaggtgc
     3901 gtggtgctct tctcgaagcg gtcccgaagt gcggcgcggt cggcggcggt gagcccgcca
     3961 tggagcggga ccacgaacac cccgctgtcg acggggccgg gcaaggctcc cggggccgtc
     4021 ggcgcctgag gcaccgcagt ccagaggatg ccttcctggt cgaggaggtg gccgatggcc
     4081 agcgtgaagt ccgaaccggt gtcgacgaga gacaagacca tctgacgagc gtcggccggt
     4141 ctcgggccgg cctgtacgta gtcaagagaa ttgcccatgc cgggcagggc cacggccggc
     4201 aggccgaccg tggcgcgagt cccgttcgat cctccggcga cctcaggtcg ggacgggcgg
     4261 cgtggtggtc ggcggcgtgg tcgtggacgt cgggggcgtg gtggtcgtgg gcggcgtggt
     4321 ggtggtggg
//
LOCUS       DAOSOA010000289         4306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20499_length_4306_cov_4.710892, whole genome shotgun sequence.
ACCESSION   DAOSOA010000289 DAOSOA010000000
VERSION     DAOSOA010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20499_length_4306_cov_4.710892"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKY13_14245"
     CDS             <1..522
                     /locus_tag="PKY13_14245"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT81151.1"
                     /translation="TSVLDGNLIGSWTATEDHPGVASAKRVDQLVAAVEAARKVSDTV
                     VVFLHWGTETKFCANQNQLDLAPRLIIAGADLVIGSHAHRVLAGGYLGSAYVGYGLGN
                     LVFKAGSGDGRDTGLLRVTVTGRRVDDAEWLPGRIGGNYLPTLLDGADRDAELATWNS
                     RRACSELADAPDS"
     gene            654..1901
                     /locus_tag="PKY13_14250"
     CDS             654..1901
                     /locus_tag="PKY13_14250"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOT81152.1"
                     /translation="MTHPTSSAAADLLSFIDASPSPYHAVAAAVARLDAAGYDAAPGT
                     PTPGQLPRRGYLADGGLLIAWDVADAALPWRIIGAHTDSPNLRIKPRPDLGGSGMRRL
                     AAEPYGGALLNSWLDRDLGLSGRVAVRESDGTVGERRFRDDRPLLRVPQLAIHLDREI
                     STTGLLLNKQQHLNPVWGLGTPVEGEFSAWLADAVGVVATDVLAWDVMCHDTQPSGFL
                     GIEEELISAPRLDNLCSCHGALSALLAARPVSTNTMIALFDHEEIGSESATGAASPRL
                     QQVLDAFGPLDAADSRVLSADMAHGTHPNYPERHEPNHPIALGGGPVVKVNVNGRYAT
                     EAITNGEFVAACRGAGVPTQVYAHRADLACGSTIGPITAARLGIATVDVGMPQLSMHS
                     SRELMAASDVDHMVTAFTAWLSA"
     gene            complement(1879..3834)
                     /locus_tag="PKY13_14255"
     CDS             complement(1879..3834)
                     /locus_tag="PKY13_14255"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HOT81153.1"
                     /translation="MTGADPTGADPTVGARRASSKVAADRVALPVGVLVGVIVATAAS
                     VGIAARLVPADGRDPSWAMLGGAVSGALAVGALRSVSVRSTTRWMVIIGALLLLRFGS
                     LAIDDASGALLMLGWIAASATALVLAARSERWMWRQGTVVSGAGSRGGRSSGGGGRLV
                     RIVVVVVCLVAAAGVGLGPAVAQGFSNGPSLGDRPSFERGSIGSAVAATERLDMRTRP
                     RLSDAVVLSVATALPSFWRSQTFDEWDGQQWTRSVRDLTVVPASGTITPPDDDLVSAA
                     ATSITQEVTVEAPYLSALPTAPSAVRVDAPTTLLQWEDGSVATNQALGKGARYRVESR
                     QRRDLAPDLLRAADAADIPADLTARYASDPVTTERVRRLALEITAGASTAYDKARAIE
                     SWLDANTRYSLDAPLAPSDADVVDDFLFVSKLGWCEQIASSFTVLLRASGVPARLAVG
                     YVPEGRDPVTQRFLVRERDAHAWTEVWFAGVGWVPFDPTAGVTLAGDTPESFGSQLSR
                     YGSWVLLGLALVLGLWSALVDGATALVRAIVRLGRARPSGRRSRRRGPVSWPAETELA
                     LERLGRRWGRDRGPAETTTSYARALGVHVGDDRLGAVGELVDRARFAAQPPGDSERAA
                     AAAVLETVDASAATSSPGEPVRPTARR"
     gene            complement(3831..>4306)
                     /locus_tag="PKY13_14260"
     CDS             complement(3831..>4306)
                     /locus_tag="PKY13_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT81154.1"
                     /translation="AAHGGDEVRSVRPYRSGDPAHLVHWPSSARVGSLVVREFDPPTR
                     PGVVLVVDLGPAVVAVVGPTVGAAPDTAVDTAVDTAVERAVAEAAGLAAAVLAFGGRV
                     LLCTHEQGRAHTAEVATPLQARRRLAAATPGPVDRPSGPPDGATTTVVFAPGVPS"
BASE COUNT          628 a         1672 c         1476 g          530 t
ORIGIN      
        1 acgtcggtcc tcgacggcaa cctcataggt tcgtggaccg cgaccgagga ccatccgggg
       61 gtggcgtccg ccaaacgtgt cgaccagctc gtcgccgccg tcgaagccgc ccgcaaggtg
      121 tccgacaccg tcgtcgtgtt cctccactgg ggtaccgaga cgaagttctg cgccaaccag
      181 aaccagctcg acctcgcgcc ccgcctgatc atcgcgggtg ccgacctcgt gatcggcagc
      241 cacgcccacc gggtgctcgc cggcggctac ctcggatcgg cctacgtcgg ctacggactg
      301 ggcaacctgg tcttcaaggc cgggagcggc gacggccgcg acaccggtct cctccgggtg
      361 acggtgacgg gccgccgcgt cgacgacgcc gagtggctcc cgggccggat cggcggcaac
      421 tacctgccga cgctgctcga tggagccgac cgcgatgccg agctggccac ctggaactcc
      481 cgtcgggcct gctccgaact cgccgacgcc cccgactcct gaccgacgac tccgaatggc
      541 ctcggccacc tcgctcaggc tcgctccctc gtccctcggg agctgcttac gctgcggtac
      601 ctcggcgatc tgatggcccc ggctgacccg tattcgctgc gagactggcg ggcgtgaccc
      661 acccgaccag ctccgccgcc gccgacctgc tctcgttcat cgacgcatcg ccgtcgccct
      721 accacgctgt cgccgcggcc gtcgcccggc tcgacgccgc cggctacgac gctgcccccg
      781 gcacgccgac gcccggccag ctcccccggc ggggctacct ggccgacggc ggcctgctca
      841 tcgcgtggga cgtcgccgac gccgcactgc cctggcgcat catcggagca cacaccgatt
      901 cgccgaacct gcgcatcaag ccccgccccg acctcggtgg ctcggggatg cggcggctcg
      961 ccgccgaacc ctacggcgga gcgctgctca actcgtggct cgaccgcgat ctcgggctgt
     1021 cgggccgcgt cgccgtgcgc gaatcggacg ggacggtcgg cgagcgacgc ttccgcgacg
     1081 accggccgct tctccgcgtc ccccaactgg cgatccacct cgatcgtgag atctccacga
     1141 ccggactcct gttgaacaag cagcagcacc tgaacccggt gtgggggctc ggcacacccg
     1201 tcgagggtga gttctcggca tggctcgccg acgccgtcgg cgtggtcgcg accgacgtcc
     1261 tggcctggga cgtgatgtgc cacgacaccc aaccctcggg gttcctcggc atcgaggagg
     1321 aactcatcag cgctcctcgc ctcgacaatc tctgctcgtg tcacggagcg ctcagcgccc
     1381 tgctggcagc acgcccggtg tcgacgaaca cgatgatcgc cctcttcgac cacgaggaga
     1441 tcggctcgga gtccgccacc ggcgccgcgt caccccggct ccagcaggtg ctcgacgcgt
     1501 tcgggcccct cgacgccgcc gattcgcggg tgctgtccgc cgacatggcg cacgggacgc
     1561 accccaacta tcccgagcgc cacgagccga accaccccat cgccctcggc ggtggcccgg
     1621 tcgtgaaggt gaacgtgaac ggccggtacg cgaccgaggc gatcaccaac ggcgagttcg
     1681 tcgcggcgtg ccgcggcgcc ggcgtcccga cccaggtgta cgcccaccgg gccgacctgg
     1741 cctgcggatc gacgatcggc ccgatcacgg cggcgcgcct cggcatcgcg acggtcgacg
     1801 tcgggatgcc ccagctgtcg atgcactcgt cgcgggagct catggccgcg tccgacgtcg
     1861 accacatggt gacggcgttc accgcctggc tgtcggcctg accggctcgc ccggactcga
     1921 cgtcgccgcc gacgcgtcga ccgtctccag tacggcagcg gcagcggctc gctcggagtc
     1981 ccccggcggt tgcgcggcga accgcgcccg gtcgacgagt tcacccaccg caccgagccg
     2041 gtcgtcgccg acgtgcaccc cgagggcacg ggcatacgac gtcgtcgtct ccgccggacc
     2101 ccggtcgcgg ccccaccgcc gcccgagccg ttcgagggcc agctcggtct ccgccggcca
     2161 gctcaccggg ccccggcggc ggcttcgacg gcccgacggc cgagcccgac cgagccgaac
     2221 gattgcccgc acgagcgctg tcgcaccgtc gacgagcgca ctccacaggc ccaggaccag
     2281 cgcgagcccg agcaggaccc acgacccgta ccgcgacaac tgcgagccga acgactcggg
     2341 ggtgtctccc gccagcgtga cccccgccgt cgggtcgaac ggaacccaac ccaccccggc
     2401 gaaccacacc tcggtccagg cgtgggcgtc gcgctcgcgc acgaggaatc gctgcgtcac
     2461 cggatcccgc ccctcgggga cgtagccgac cgccaggcgg gccgggacgc cgctcgcccg
     2521 caagagcacc gtgaagctgc tggcgatctg ctcgcaccat cccagcttcg acacgaacag
     2581 gaagtcgtcg acgacgtcgg cgtcggacgg cgccagcggg gcgtcgaggg agtaccgggt
     2641 gttggcgtcg agccacgatt cgatcgcacg cgccttgtcg tacgcggtcg acgcgccggc
     2701 ggtgatctcg agggccaacc ggcggacccg ttcggtcgtg accggatccg atgcgtagcg
     2761 ggccgtcagg tcggcgggga tgtcggccgc gtcggcggcg cgaagcaggt cgggggcgag
     2821 gtcacgtcgc tgacgactct cgacgcggta ccgggcgccc ttgccgagtg cctggttcgt
     2881 cgcgaccgaa ccgtcctccc actgcagcag cgtggtgggc gcgtcgacgc gcaccgcgct
     2941 cggtgccgtc gggagcgccg acaggtacgg cgcctcgacc gtgacctcct gggtgatcga
     3001 cgtcgcagcc gccgagacga ggtcgtcgtc gggcggggtg atcgtccccg acgccggcac
     3061 caccgtcagg tcacggacgc tgcgcgtcca ctgctggccg tcccactcgt cgaacgtctg
     3121 ggagcgccag aagctcggga gtgccgtcgc caccgacagc accacggcgt cgctcaaccg
     3181 cggccgcgtc cgcatgtcga ggcgttcggt cgcggcgacc gcgctgccga tcgaaccccg
     3241 ctcgaacgac ggccggtccc cgaggctggg gccgttcgag aacccctgcg ccacggccgg
     3301 accgagcccg actccggccg cggcgaccag gcacaccaca acgaccacga tccgaacgag
     3361 gcggcccccg ccgccgctgg atctgccccc gcgggacccg gcacccgaca ccacggtgcc
     3421 ctgccgccac atccaccgtt ccgatcgtgc ggccagcacc agcgcagtcg ccgacgcggc
     3481 gatccacccg agcatcagca gcgcccccga cgcgtcgtcg atggccaacg acccgaaccg
     3541 gaggagcaac agggcgccga tgatgaccat ccatcgcgtc gtggagcgga ctgacaccga
     3601 gcgcagcgcg ccgacggcca gggcgcccga gaccgccccg ccgagcatgg cccacgacgg
     3661 atcgcgtccg tcagccggaa cgagacgggc agcgatcccg accgaggcgg cggtcgccac
     3721 gatgacaccg accaggacgc ccaccgggag cgcgacgcgg tccgcagcaa ccttcgagga
     3781 ggctcgccgg gccccgacgg tcgggtcggc accggtcggg tcggcgccgg tcacgacggc
     3841 acccccggag cgaacacgac cgtcgtggtg gcaccgtcgg ggggtccact cgggcgatcg
     3901 acggggccgg gggtcgctgc ggcgagacgt cggcgtgctt gcaacggcgt ggcgacctca
     3961 gcggtgtggg cacggccctg ctcgtgggtg cagagcagca cgcggccccc gaacgccaac
     4021 acggccgcgg ccagaccggc ggcctcggcc acggcccgtt cgaccgcagt gtcgaccgcg
     4081 gtatcgactg cggtgtcggg agcggcaccg acggtggggc cgacgaccgc gacgaccgcg
     4141 gggccgaggt cgacgaccag cacgacgccc ggccgtgtgg gtgggtcgaa ctcgcgcacg
     4201 acgagcgacc cgacgcgggc cgacgacggc cagtgcacca ggtgggcggg gtcgcccgac
     4261 cggtacgggc gcaccgaccg cacctcgtca ccgccgtggg cggccg
//
LOCUS       DAOSOA010000290         4289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20586_length_4289_cov_11.021493, whole genome shotgun
            sequence.
ACCESSION   DAOSOA010000290 DAOSOA010000000
VERSION     DAOSOA010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20586_length_4289_cov_11.021493"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..3503
                     /locus_tag="PKY13_14265"
     CDS             219..3503
                     /locus_tag="PKY13_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="HOT81155.1"
                     /translation="MTDQPGAAPADDNPNDAPKQIATLSAADWPEPPAIDPALMAGND
                     GLGQYGDAIGQFLTDTGMLTPAHAQYLRDRGVARDVAIARGYTSVVTRAGVKALGPFS
                     ESVARTVNGPDGCHAIAIPLYHAGRNDPSLYQLRPDRPRMAKSPNPAEKAKTMKFEIP
                     GGVRRGSEHGDLPADVHPMCHHEATDEDTPLLLTEGVVKADAALTAARREGLGIVPVA
                     LTGVTLGFSSTRNVDDDGNEITHRELTEALTSIPFVGRVVYLCWDSDWHSKRQVRSSL
                     FRTGDLLAAAGAQVHYVNLPAAGTDKCGLDDYLVQHSDDPAPLATLLSQHLMDNGDLV
                     SPEQEEADEERDTAELDLFEIDPERLRIYGYTTRQRGASDTKTRRLDVKLDALPEIAS
                     AVSVARITDLQLEETEDQFIVVVRWRDDAGLVRSREVIVPTTDFDNVRAWMALHAATK
                     MRHFSPTPADERKIAETILHAARHAPHEMRAVSTGWYRSEIPAPGSPTTTPTGAPRDT
                     RWRYVTGRGWFGPDFTFGDDVTCESDSQAKEVIVPPPGTDLELLRSATPYDGEDGLIA
                     LSAALSSDERQQSAAAAFRDWWSAWVMDGPDSIGEQTYAEMKATGTDPTRERRQFDLS
                     KILRLGAVAIARAITPGAPNKGLLLLLGLPGSGKTLTARCWTSAFGNEFGHRPYLDLR
                     STSAGIEVQLADANNALVVVDDFRIGDTKETERMNAVVDGLARSAHDGSRRQRSTRDL
                     RTMAAPVVNASVILTGETMPTVSEATNSTAQRMIIVSFPHPSTIDPRVTRRLATLAEE
                     TPEPQQLALSAMLSWVANCLADIGPGEIRQQVREFRNTRLKVDVDMFRDSIVAQAAEL
                     TRAPISARTKVIVEDFAVGGATLLGCAESFGVPPDELDRATALLTEMFAWVTAQTANS
                     IASTSPAAKLVDSITAALDSGEAYLSLVDGQPPRDHEVALHWGWKPEDGKLKHGSRQI
                     GWVLQTDVGGEPTAVIALQPEALKDVFRRSGQGLGQLSPKQIAEQLNTLKMTVSELDP
                     DTGRTVARETTALAVKPESTTPTVRLRVGEAPKSVVAMIPVAMGIPIPDQFEPAPSSA
                     GAGEEF"
     gene            3689..>4289
                     /locus_tag="PKY13_14270"
     CDS             3689..>4289
                     /locus_tag="PKY13_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81156.1"
                     /translation="MFDTAITVPRAGLPDANRLRRRSLLFRLWVWTGVVLLPLALLAL
                     FIGGGSKTAGTDTSAATSPGRAAATVALTSWLRDASTPLPGGVILGWTGSVQLGEPVT
                     EEGSELTTTITLERFDVAAAGVIYDASVQVATDSRGGSIAISTPSATPKAPPANDDWE
                     DVGPWAGFGGSNAQSTPAIVKAVELWAEAFTSGDADTLRL"
BASE COUNT          763 a         1620 c         1292 g          614 t
ORIGIN      
        1 ggtcaccgtc gacaacgtcg acctacagct ccaacacgtc gacttcgccc tcgtcgcatc
       61 gtcggtgtcg ctcaacaaca ccgacctcaa cccgtccgcc gtcgccgaac tggcggcacg
      121 cttcgcagcg agacggagct cacggtgaga tcgcgccgac agtctggccg agaccccgtc
      181 cggccatagc tgccgcagtc accgcatagg ccaggcctgt gaccgatcaa ccaggggctg
      241 cgcccgccga cgacaacccg aacgacgcgc cgaagcagat cgccacactg tcggccgccg
      301 actggcccga accgcccgcg atcgacccgg ccctcatggc gggcaacgac ggtctcggcc
      361 aatacggcga cgccatcggc cagttcctca ccgacaccgg gatgctcaca ccagcccacg
      421 cccaatacct ccgcgaccgc ggcgtcgccc gagacgtcgc gatcgcccgc ggctacacct
      481 cggtggtcac ccgcgccggg gtcaaggccc tcgggccgtt ctccgaatcg gtggcacgca
      541 ccgtcaacgg gcccgacggc tgccacgcca tcgccattcc gctctaccac gccggccgca
      601 acgatccgtc gctctaccag ctgcggcccg accgcccccg gatggcgaag tcccccaacc
      661 cggccgagaa ggccaagacg atgaagttcg agatccccgg cggagtgcgc cgcggctccg
      721 agcacggcga cctccccgcc gacgtccacc cgatgtgcca ccacgaagcc accgacgagg
      781 acactcccct cctcctcacc gaaggcgtcg tgaaggccga cgccgccctc accgcggcgc
      841 gtcgcgaagg gctcggtatc gttccggtcg cactgaccgg cgtgaccctc ggcttctcct
      901 ccaccagaaa cgtcgacgac gacggcaacg agatcactca ccgcgaactc accgaggctc
      961 tgacgtcgat ccccttcgtc ggccgcgtcg tgtacctctg ctgggattcg gactggcact
     1021 ccaaacgcca ggtccgttca tcgctgttcc gtaccggcga cctcctggcc gccgccggcg
     1081 cccaggtcca ctatgtgaac cttcccgccg ccggcaccga caagtgcggg ctcgacgact
     1141 acctggtgca acacagcgac gatcccgcac ccctcgcaac gctgctgtcc caacacctca
     1201 tggacaacgg cgacctcgtc tcacccgagc aggaggaagc ggacgaggaa cgcgacacag
     1261 ccgagctcga cctgttcgag atcgacccgg agcggctccg aatctacggg tacaccaccc
     1321 gtcagcgcgg agcatcggac accaagaccc gacggctcga cgtgaagctc gacgctctcc
     1381 ccgagatcgc gtcggcggtc agcgtcgccc gcatcaccga cctccagctc gaggagaccg
     1441 aggaccagtt catcgtcgtg gtccgctggc gagacgacgc cggcctggtc cgaagccgcg
     1501 aggtcatcgt tccgacgacc gacttcgaca acgtccgagc ctggatggcc ttgcacgccg
     1561 ccaccaagat gcggcacttc tcccctactc ctgcggacga acgaaagatc gccgagacga
     1621 tcctgcacgc cgcccgtcac gcaccccacg agatgcgcgc cgtgtcgacg gggtggtacc
     1681 gctcggagat ccccgccccc gggtcgccga ccaccacgcc gactggcgct ccacgcgaca
     1741 cccgctggag gtacgtgacc gggcgcggct ggttcggccc cgacttcacg ttcggcgacg
     1801 acgttacctg cgaaagcgac tcccaggcca aagaggtcat cgtcccaccg cctggaaccg
     1861 atctcgaact cctccgctcc gcgacgccct acgacggcga ggacggactc attgccctct
     1921 cggcggccct cagctccgat gagcgccaac agagcgctgc cgcggccttc cgggactggt
     1981 ggtccgcgtg ggtgatggac ggcccggact cgatcggcga gcagacctac gccgagatga
     2041 aggccaccgg caccgaccca acccgagaac gacgccagtt cgacctgtcg aagatcctgc
     2101 gcctcggcgc cgtcgcaatc gcgcgggcca tcacgccggg cgcccccaac aagggcctgc
     2161 tcctgctgtt ggggctgccg ggatcgggca agacactcac cgcacggtgc tggacgtcag
     2221 cgttcggcaa cgagttcgga caccgcccgt atctcgacct gcgctcgacg tccgccggca
     2281 tcgaggttca gctcgcggat gccaacaacg cgctcgtcgt cgtcgacgac ttccggatcg
     2341 gtgacaccaa ggagacggaa cggatgaacg ccgtcgtcga cggcctggcc cgctccgccc
     2401 atgacgggtc gcgccgccaa cgatcgactc gggacctccg cacgatggcc gcaccagtcg
     2461 tcaacgcttc ggtcatcctc accggcgaga cgatgccgac ggtgtcggaa gcgacgaact
     2521 cgaccgctca gcgcatgatc atcgtgtcgt tcccccaccc gtccacgatc gatcccagag
     2581 tcacgcgacg cctcgcaacg ctggccgaag agacgcccga accccaacag ctcgcccttt
     2641 cggccatgtt gagctgggtc gcgaactgcc tcgccgacat cgggcccggt gagatccgcc
     2701 aacaggtccg ggagttccgc aacacccgac tcaaagtcga cgtcgacatg ttccgcgaca
     2761 gcattgtcgc ccaggccgcc gagttgaccc gggcgccgat ctcggcccga accaaggtga
     2821 tcgtcgaaga cttcgccgtg ggcggtgcga cactgctcgg atgcgccgag tccttcggtg
     2881 tgccgcccga cgaactggat cgggccacag cgttgctcac cgaaatgttc gcgtgggtca
     2941 ccgcccagac cgcgaactcg atcgccagca ccagtcccgc cgcgaagctg gtcgactcca
     3001 tcacagccgc tctcgactcg ggcgaggcgt acctgtccct cgtcgacggc cagcccccac
     3061 gtgaccacga ggtcgccttg cactggggat ggaagccgga agacgggaag ttgaagcacg
     3121 ggagccgtca gatcggttgg gtgcttcaga ccgacgtcgg tggagaaccg accgccgtca
     3181 tcgcgctgca accagaagca ctcaaggacg tgttccgtcg atcaggccaa ggcctcggtc
     3241 agctgtcccc gaagcagatc gccgaacagc tgaacacctt gaagatgacc gtcagcgaac
     3301 tggaccccga cacgggccgc accgtcgctc gagaaaccac cgccttggcc gtcaagccgg
     3361 aatcaacgac tccgacagtg cgtctccgcg tcggcgaggc acccaagtcg gtggtggcca
     3421 tgatcccggt tgccatgggc attccgatcc ccgaccagtt cgaaccagcg ccttcatccg
     3481 ccggcgctgg agaggagttc tgatggcgcg caagcgacca gacaacacgc caccggtctt
     3541 cggttccgac cgcctcggtg gacaccgcga cgacggctgg ggcatcgatc atcctcagcc
     3601 ggatcgacca cccttgtcac cggaggacga tcgactctca ccagtccgcg gcgaggggcg
     3661 gcgccgcaga acgcctcgat cgtcgacggt gttcgacacc gcgatcacgg tgccccgagc
     3721 aggcctaccc gacgcgaacc gcctccgtcg gcggtcgttg ctgttccggc tgtgggtctg
     3781 gaccggtgtc gtcctactcc ccctggcgct gctcgcgctg ttcatcggcg gcgggtccaa
     3841 gaccgccggc accgacactt cggctgccac ctcccccgga cgcgccgcag caaccgtggc
     3901 gctcacgtcg tggttgcgag acgccagcac tccgctgccc ggcggagtga tcctcggctg
     3961 gaccgggtca gtccaactcg gcgaacccgt caccgaggag ggctccgagc tcaccaccac
     4021 gatcaccctt gagcgcttcg atgtcgccgc ggccggcgtg atctacgacg ccagcgtcca
     4081 ggtcgcgacg gactcccggg gtggttcgat tgcgatctcg acgccctcgg ccactcccaa
     4141 ggccccgcct gccaatgacg actgggaaga tgtggggccg tgggccggat tcggcggttc
     4201 caacgcccag tcgaccccgg cgatcgtcaa ggctgtggaa ctgtgggccg aggcgttcac
     4261 ctcgggcgac gcagacacgc tgcggctct
//
LOCUS       DAOSOA010000291         4280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20648_length_4280_cov_6.443077, whole genome shotgun sequence.
ACCESSION   DAOSOA010000291 DAOSOA010000000
VERSION     DAOSOA010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20648_length_4280_cov_6.443077"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKY13_14275"
     CDS             complement(<1..410)
                     /locus_tag="PKY13_14275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT81157.1"
                     /translation="MAAADAPSSSPARTVYRGARWPGDVAVVDGRIVAVGSVPSGPGD
                     VDIDCTGTVITAGLVNTHHHLYQWMTRGWASGCDLFTWLTTLYPVWGRLDVEDIHAAA
                     LVGLSELAMTGCTTAADHHYLVPHGDDAVFDAIV"
     gene            468..794
                     /locus_tag="PKY13_14280"
     CDS             468..794
                     /locus_tag="PKY13_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81158.1"
                     /translation="MTRERISATIEADLLAAGRAAVADGRADNLSAWVNEALRLRVDL
                     DRRLVAFDEFLSDYESEHGEITEAEMAAATRAARSRAVVVRATGTPAPTPRPTRRTRP
                     SKRGAA"
     gene            791..1171
                     /locus_tag="PKY13_14285"
     CDS             791..1171
                     /locus_tag="PKY13_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81159.1"
                     /translation="MTLVLDAGAFVAVERGDREIIALLKSERLQGRSAVTHGGVVAQV
                     WRGGDDRQAHLARLLRAVEVVPLDDALGRAAGILLGVAGTSDAIDAAVVLLSRDRDEV
                     LTSDPHDLAILAEAAGLQIDLVPV"
     gene            complement(1184..2764)
                     /locus_tag="PKY13_14290"
     CDS             complement(1184..2764)
                     /locus_tag="PKY13_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81160.1"
                     /translation="MIDLDDIDPAIESRLRSSLLARADLASGVTGPDPALFRTSAPRR
                     VRRRNALLVAAAIALVIAGLAVLLRSPDGRRVDVVPATTVPTTAEDVAFVLDRWPAGF
                     GAVESAGIDDEDLSNGVEDRRVLGRDGNAEVESLLQMAPLETSERVPTGTEDLEIGGR
                     KAQWSDRRGLPTLSVEVRPGLTLTLASVRVDRDELVELVPAFDAADGAFDERRLPRGW
                     SEIPDPMWSSALTGVGSKGRRGWISASIIEDNRLVASLSAAWVSVPDPDAVLEMVEGL
                     RGGERSTIAGRPSLVLPDPGLTAPTVRLVMVLDDGHLAVVVGSDLSREEVSASAASMR
                     PASEAEWADLIAGLPDQAPGAAAGRVVDGVELSPTTKDVVTNGRQGAYRWVLTTEDFG
                     SPGVEAGANGGYTVDVRSPDGTQSHGGTGWGFPNGGGLSTTRDGGLMVYMTVTSGDFT
                     VDRLERDGQAIETVVVPVVDGSLQVVFGWEPAIDVGPQPSVVVTGTLADGTRIPPPFS
                     TQPTPGSGMSFPESIGGN"
     gene            complement(2761..3273)
                     /locus_tag="PKY13_14295"
     CDS             complement(2761..3273)
                     /locus_tag="PKY13_14295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT81161.1"
                     /translation="MEPLTAHSIPGEPSVEATTPERAFDAVFAAEYVPLVRLATAVCG
                     DVGVAEEVTQEAFTSAWRRWDRLDRPGAYLRRAVTNGAIDATRRRGRSDRRQRDLRVV
                     TPVTADAPTDPLWDLIDALPDVRRAVVVLRFYADLTIPEIAEIVERPLNTVKTDLRRA
                     LSTLSKELSE"
     gene            3406..>4280
                     /locus_tag="PKY13_14300"
     CDS             3406..>4280
                     /locus_tag="PKY13_14300"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HOT81162.1"
                     /translation="MSDDPLALPQVSESRLMARLEALAEIGDTGDGGCARLALTDEDR
                     AGRDLVVGWMEDLGLEVTVDGIGNVVATQPGFDSEIAPVMCGSHIDTVRTGGRYDGNY
                     GVLAGLEVIEAAQTAGLELTRPLAVAFFTDEEGARFAPDMLGSLAYAGGIGIEDALDI
                     VGIDGVTVGDELRRIGYAGGAPLPGRAPHAFVEVHVEQGPVLEAEGLTIGAVTGVQGI
                     SWQEITISGQSNHAGTTPMSMRHDAGFAAFAIGAFVRGLAIEIGPPQVGTVGKIDLEP
                     NLVNVVARTARLTVDL"
BASE COUNT          672 a         1536 c         1458 g          614 t
ORIGIN      
        1 tcgacgatgg cgtcgaacac ggcgtcgtcg ccgtggggga cgaggtaatg gtggtccgcg
       61 gcggtcgtgc agccggtcat cgccaactcg ctcaacccga cgagcgctgc agcgtggatg
      121 tcctcgacgt cgagtcgacc ccacaccgga tagagggtgg tcagccacgt gaacaggtcg
      181 caccccgacg cccacccccg cgtcatccac tggtagaggt ggtggtgggt gttgacgagc
      241 ccggcggtga tgacggtgcc ggtgcagtcg atgtcgacgt cacccggtcc ggacgggacg
      301 ctcccgacgg cgacgatgcg accatcgacc acggcgacgt cgcccggcca gcgggctccc
      361 cggtagacgg tgcgagcggg agaggacgac ggggcgtctg cggctgccat ggcgaaacgg
      421 tagtggttcc tcgtatgagt gtcatacaca ctcagtagga tgaatgtatg acgcgagagc
      481 ggatctctgc caccatcgaa gccgacctcc tcgcggccgg acgcgccgcc gttgccgacg
      541 gccgcgccga caacttgagc gcatgggtca acgaggcgct ccggcttcgg gtcgacctcg
      601 accggcgtct ggtggcgttc gacgagttcc tgtctgacta cgagtccgaa cacggtgaga
      661 tcaccgaggc cgagatggcc gccgccacca gagccgcccg ctcgcgggcc gtcgtcgtgc
      721 gggcaacggg caccccggcg ccgacccccc gaccgacccg acggacccgc ccgagcaagc
      781 ggggggcggc atgacgttgg tgctcgacgc cggcgccttc gtcgccgtcg agcgcggcga
      841 ccgcgagatc atcgccctgc tcaagagcga acggctgcag ggacgaagcg ccgtcaccca
      901 cggcggcgtc gtggcgcagg tctggcgagg cggtgacgat cgccaggccc acctggcgcg
      961 gctgctccga gctgtcgagg tcgtcccgct cgacgacgcg ctcggcaggg ccgccggcat
     1021 cctgctcggg gtggcgggaa cgtccgacgc gatcgacgca gccgtggtgc tcctcagcag
     1081 ggatcgcgac gaggtgctga catcggaccc tcacgacctc gcgatcctgg ccgaagccgc
     1141 cggcctccag atcgacctgg tcccggtctg acagctgagg agttcagttg cctccgatcg
     1201 actcggggaa cgacatcccc gagcccgggg tgggctgagt cgagaacggc ggcgggatcc
     1261 tggtcccatc tgccagcgtg cccgtgacca ccacgctcgg ttgtgggccg acgtcgatcg
     1321 ccggctccca gccgaagacc acctgcaacg acccatcgac gaccggaacg acgacggtct
     1381 cgatcgcttg tccgtcgcgt tcgagccgat cgacggtgaa gtcgccgctc gtcaccgtca
     1441 tgtagaccat gagcccgccg tcgcgggtcg tcgacagacc gccgccgttc gggaatcccc
     1501 agccagtgcc tccgtgagac tgtgtgccgt cggggctccg gacatcgacg gtgtaaccac
     1561 cgttcgcacc tgcctcgact ccgggagaac cgaagtcctc ggtcgtcagc acccagcgat
     1621 acgcaccctg ccgcccgttc gtcaccacgt ccttcgtggt cggggagagc tcaacgccgt
     1681 caacgacccg gccggccgcc gcgcccggag cctggtcggg caagccggcg atcaggtccg
     1741 cccattcggc ctcggaggcc gggcgcatcg acgccgccga tgccgacacc tcctcacgcg
     1801 acaggtcgct gccgacgacg acggcgaggt ggccgtcatc gagcaccatc acgagccgca
     1861 cggtcggagc cgtgagtccc ggatccggga gcaccagcga aggtcgccct gcgatcgtcg
     1921 atcgctcgcc tccccgcagg ccctccacca tctcgagcac tgcgtcagga tcgggaacgc
     1981 tgacccacgc cgccgagagc gacgcgacca ggcggttgtc ctcgatgatc gaggcgctga
     2041 tccaaccgcg gcgccccttc gaaccgacac cggtcaacgc cgatgaccac atggggtcgg
     2101 gtatctcgct ccaccctcgt ggcagacgcc gctcgtcgaa cgcgccgtcg gcggcgtcga
     2161 aggccggaac cagctctacc agctcatcgc ggtcgacgcg cacgctcgcg agggtcaacg
     2221 tgagacccgg ccgcacctcc acgctcagtg tcggtagtcc ccgccggtcg gaccactgcg
     2281 ccttccgacc gccgatctcg agatcctccg tacccgtcgg cacccgctcc gacgtttcca
     2341 acggcgccat ctgcaggagg gactcgacct cggcgtttcc gtcgcgaccg agaaccctgc
     2401 ggtcttccac cccgttggag aggtcttcgt cgtcgatgcc ggcgctctcg acggcgccga
     2461 atcccgccgg ccaacggtcg agcacaaacg ccacgtcctc cgctgtcgtc ggcaccgtcg
     2521 tggccggcac cacgtcgaca cggcggccgt ccggcgaccg cagcagcaca gcgaggccgg
     2581 cgatgacgag ggcgatcgcc gccgcgacga ggagcgcgtt gcggcgacgc acccgcctcg
     2641 gagcggacgt ccggaacagc gccgggtcgg gaccggtaac gccggacgcc aggtcggcgc
     2701 gggcgagcaa cgaagaacga aggcgcgatt cgatggctgg atcgatgtcg tcgagatcga
     2761 tcattcgctc agctccttgg agagggtcga gagtgcccga cggaggtcgg tcttcacggt
     2821 gttgaggggt cgctcgacga tctcggcgat ctcggggatc gtcaggtcgg cgtagaagcg
     2881 gaggaccacc acggcccgcc gcacgtccgg caacgcgtcg atcaggtccc acaacgggtc
     2941 ggtcggggcg tcggcggtga cgggagtgac gactcgcagg tcccgctggc gccggtcgga
     3001 gcggccacgg cggcgcgtgg cgtcgatggc gccgttcgtc acggcccgcc gcaggtaggc
     3061 gccgggccgg tcgaggcggt cccaccggcg ccacgccgag gtgaacgcct cctgcgtcac
     3121 ctcctcggcc acgcccacgt ccccacagac cgccgtcgcc aggcgcacga gcggcacata
     3181 ctccgcagcg aacacggcat cgaaggctcg ctcgggcgtc gtggcctcca cgctcggctc
     3241 gcccgggatc gagtgcgcgg tcagcggttc cacacctcca cgacgaccga gcacgcccga
     3301 tcgttgcacg gcgttgaaca atcgtcagca gcgatcgcgc gaactgttca cggacccggg
     3361 ccaggtttta cttttcgtaa aggagcgcgc cggtagcctt gtgccatgag cgacgatccc
     3421 ctcgcgctgc cccaggtgtc ggagtcccgg ctgatggcgc gcctcgaggc tctcgccgag
     3481 atcggcgata ccggcgacgg cggatgcgcc cggctggcgc tgaccgacga ggaccgggcg
     3541 ggtcgcgacc tggtcgtcgg ctggatggag gacctcgggt tggaggtcac cgtcgacggc
     3601 atcggcaacg tggtcgccac ccaaccgggt ttcgattccg agatcgcgcc cgtgatgtgc
     3661 ggcagccaca tcgacaccgt ccgcaccggc ggacggtacg acggcaacta cggcgtgctc
     3721 gccggtctcg aggtcatcga ggcggcacag accgccggac tcgaactcac ccgcccgctc
     3781 gcggtcgcgt tcttcaccga cgaggagggc gcccgcttcg cccccgacat gttgggctcg
     3841 ctggcgtacg ccgggggcat cggcatcgag gatgcgctcg acatcgtggg catcgacggc
     3901 gtcaccgtcg gtgacgagtt gcggcgcatc gggtacgccg gcggcgctcc ccttcccggt
     3961 cgggcgcccc acgcgttcgt cgaggtccac gtcgagcagg gtccggtgct cgaagccgag
     4021 gggctcacca tcggcgctgt caccggcgtg cagggcatct cgtggcagga aatcacgatc
     4081 tcgggtcagt ccaaccacgc cggcaccacc cccatgtcga tgcggcacga cgccgggttc
     4141 gccgcctttg caatcggggc gttcgtacgc gggctggcca tcgagatcgg accaccccag
     4201 gtcggcacgg tcggcaagat cgacctcgaa cccaatctcg tcaacgtcgt cgcccgcacc
     4261 gcccgcctca ccgtcgacct
//
LOCUS       DAOSOA010000292         4274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20677_length_4274_cov_5.976772, whole genome shotgun sequence.
ACCESSION   DAOSOA010000292 DAOSOA010000000
VERSION     DAOSOA010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20677_length_4274_cov_5.976772"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKY13_14305"
     CDS             complement(<1..379)
                     /locus_tag="PKY13_14305"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOT81163.1"
                     /translation="MSHFHANQLLIAKPDLTDPNFSRTVVHLVEHDAEGALGVVLNRP
                     MTIPVSEHFESLVAAVSYPPLFFEGGPVASGSVVAIGSSGGAPPRLVDIDGLLGGSTP
                     MPDQLRLFAGYAGWSAGQLEGELL"
     gene            complement(395..1627)
                     /locus_tag="PKY13_14310"
     CDS             complement(395..1627)
                     /locus_tag="PKY13_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT81164.1"
                     /translation="MTSTIDSETAAGDTKTIPRIISVDDHVVEPPHVWQTWLPEKFRA
                     AGPRIERRGIGHMKHIGGGAYEQTFDPAGPPADCWVFEDLVYIHKRHVAAVGYSRDEM
                     TMTPMTYDEMRPGCYEVTARVEDMLMNHTDASLCFPTFPRFCGQTFTEAKDRELGLAC
                     VKAYNDWMIEEWCGESGGHLIPLCIVPLWDADLAAEEVRRNAARGCHAVCFSEIPPHL
                     GLPSIHSGFWDPFFQACQDTQTTVNMHIGSSSRMPATSPDAPVAVAASLSFNNSMASL
                     TDFLFSGVLVRFPELTLAYSEGQIGWLPYILERVDDVWREHRAWGGVADLIPEPPSTY
                     YFRQVYGCFFRDNHGLESLHRVGVDNITFETDYPHTDTTWPNTLGVATAMMGHLPEDI
                     QYKILRGNAIKMLHLDMK"
     gene            complement(1631..2026)
                     /locus_tag="PKY13_14315"
     CDS             complement(1631..2026)
                     /locus_tag="PKY13_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOT81165.1"
                     /translation="MTTSDQRGVLGNGDFVSPLYEDRPWGSYTVLADETSHKVKSITV
                     APGQRLSYQRHSHRSEHWFVISGDATVTLDGVVFHVLPGHYADIPQGVAHRLENQGTD
                     PLVIIEVQYGDYFGEDDIIRLSDDYGRAE"
     gene            complement(2044..2547)
                     /locus_tag="PKY13_14320"
     CDS             complement(2044..2547)
                     /locus_tag="PKY13_14320"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT81166.1"
                     /translation="MTADLTPALLVELEKIKRLKYAYFRCLDQKLWDEMTELFVDDAT
                     AAYSGGKYHYAGRDEIVGFMRKNMSRDAFHTSHRVHHPEIEIDGDEATGTWAMEDVNV
                     DSEYDFYLTGAGFYVDRYVRADGVWKIAHTGYKRTFETIMPMSKNGMSVTASWWTTGG
                     QSALEVQ"
     gene            2609..>4274
                     /gene="hrpB"
                     /locus_tag="PKY13_14325"
     CDS             2609..>4274
                     /gene="hrpB"
                     /locus_tag="PKY13_14325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOT81167.1"
                     /translation="MTPSPPESSLAAVIPDLATALTGSGRAVLVAPPGTGKTTRVPLA
                     LLDAPWLDGRILMLEPRRVAARAAATYMAGLLGEAVGATVGYRTRGDSKVGRATRVEV
                     VTERLLTRRLQQDPSLEGIGLVIFDEVHERSLDTDLGLALCLDVAGSLRDDLRVLAMS
                     ATPDTEAIAGLLGDESGPAPIIESSGVAHPVDIVYLGLDRRRRLDDEVADAVGRALRE
                     APDGDVLVFLPGRREIRWTAERLAARSIETVPLTAGLDPSSLDGALRPRPDGRRKVIL
                     ATSVAQTSLTIDGVRVVVDAGLERSARFDPARGMSGLVTVPVSRAAADQRAGRASRQA
                     PGRCYRLWSEPEQASRRAQSEPELAVADLCALVLDVAAWGVTDPTGLRWVTPPPVAGW
                     AVAAATLRAVGAVGADGRITADGRMMSDLGAHPRLGRLMIDAARVGDDDTGTLLAALI
                     EAGVRGDLRRALDRPDREVERVAERWRRSLRSLRADGPVGARGDDSTPVPAPAPGGDE
                     AVGALVAAAFPDRVGRARDGSRTRYLLASGVGASVPEGSPLAGHDWL"
BASE COUNT          627 a         1418 c         1544 g          685 t
ORIGIN      
        1 ccaggagctc accttcgagc tggccggctg accagcccgc gtagccggcg aagagccgga
       61 gctggtcggg catcggcgtg gaaccgccga gcaggccgtc gatgtcgacg agtcgaggtg
      121 gcgctccccc actcgacccg atcgcgacga cgctgccgga ggcgacgggc ccaccctcga
      181 agaacagagg cgggtaggac acggcagcaa cgagcgactc gaagtgttcc gacaccggga
      241 tcgtcatcgg gcggttcagc accacaccga gcgcaccctc ggcatcgtgc tcgacgaggt
      301 gcacaacggt gcgggagaaa ttcggatcgg tcaggtccgg cttggcgatc aacaactggt
      361 tggcgtggaa gtgcgacacc cgaccggacc tccgctactt catgtccagg tggagcatct
      421 tgatggcgtt gccccggagg atcttgtact ggatgtcctc ggggaggtgg cccatcatcg
      481 ccgtcgcgac ccccaacgtg ttgggccacg tggtgtcggt gtgggggtag tcggtttcga
      541 acgtgatgtt gtcgacgccg acacggtgga gtgattcgag gccgtggttg tcgcggaaga
      601 agcagccgta gacctgccgg aagtagtagg tcgaaggcgg ttcggggatg aggtcggcga
      661 cgccgcccca ggcccggtgc tcacgccaca cgtcgtcgac gcgctcgagg atgtagggca
      721 gccagccgat ctggccctcg ctgtacgcaa gggtgagctc gggaaagcgg acgaggaccc
      781 cggagaacag gaagtcggtg aggctcgcca tcgagttgtt gaagctcagg ctggcggcga
      841 cggcgacggg ggcatcggga gatgtcgccg gcatgcggct ggaggaaccg atgtgcatgt
      901 tgaccgtggt ctgggtgtcc tgacaggcct ggaagaacgg gtcccagaag ccggagtgga
      961 tcgacggaag gcccaggtgg ggcgggatct cgctgaagca gacggcgtgg cagccgcggg
     1021 cggcgttgcg gcggacctcc tccgcggcga ggtcggcgtc ccacaggggc acgatgcaca
     1081 acgggatgag atggccgccg gactcgccgc accattcctc gatcatccag tcgttgtagg
     1141 ccttgacgca ggcgaggccg agttcgcggt ccttggcctc ggtgaaggtc tggccacaga
     1201 agcgggggaa ggtcgggaag cacagcgagg cgtcggtgtg gttcatcaac atgtcctcga
     1261 cgcgggccgt gacctcgtag cagccgggac gcatctcgtc gtaggtcatg ggcgtcatcg
     1321 tcatctcgtc gcggctgtaa ccgacggcgg cgacgtgacg cttgtggatg tagacgaggt
     1381 cctcgaacac ccaacagtcg gcgggcggac cggccgggtc gaaggtctgt tcgtacgccc
     1441 cgccgccgat gtgtttcatg tggccgatgc cacgacgttc gatgcggggg ccggcggcgc
     1501 ggaacttctc gggaagccag gtctgccaga cgtggggcgg ttcgacgacg tggtcgtcga
     1561 cgctgatgat ccgcgggatc gtcttggtgt cgccggcggc ggtctccgaa tcgatcgtcg
     1621 aggtcatggg ctactccgct cgtccgtagt cgtccgacag cctgatgatg tcgtcctcac
     1681 cgaagtagtc gccgtactgc acctcgatga tgacgagggg gtcggtgccc tggttctcga
     1741 ggcggtgcgc cacaccctga gggatgtcgg cgtagtggcc gggcagcacg tggaacacca
     1801 caccgtcgag cgtgacggtg gcgtcaccgc tgatcacgaa ccagtgctcg gagcggtgcg
     1861 aatggcgctg gtagctcaag cgctggcccg gcgcgacggt gatcgacttg accttgtggc
     1921 tcgtctcgtc ggcgagcacc gtgtacgacc cccacggacg gtcctcgtag aggggcgaca
     1981 cgaagtcgcc attgccgagc actccgcgct ggtcgctcgt ggtcatctcc ggcatctcgc
     2041 tcactactgc acctccagcg ccgactgccc gccggtggtc caccagctgg ctgtcacgct
     2101 cattccgttc ttcgacatgg gcatgatcgt ctcgaaggtc cgcttgtatc cggtgtgggc
     2161 gatcttccac accccgtcgg ccctgacgta ccggtcgacg tagaagccgg cgccggtcag
     2221 gtagaagtcg tactcgctgt cgacgttgac gtcttccatc gcccaggtgc cggtggcctc
     2281 gtccccgtcg atctcgatct cgggatggtg cacgcggtgg ctggtgtgga acgcgtcgcg
     2341 gctcatgttc ttgcgcatga acccgacgat ctcgtcacgg ccggcgtagt ggtacttgcc
     2401 accgctgtag gcggccgtgg cgtcgtcgac gaacagctcg gtcatctcgt cccacaactt
     2461 ctggtcgaga caacggaagt aggcgtactt gaggcgcttg atcttctcga gctccaccag
     2521 cagcgcgggg gtcaggtccg cagtcattgg gcagagcata gatgccccgg acagatggac
     2581 acctgtccag gacaggtagg ttcgcgtcgt gacaccgtcg ccccccgaat cgtcgctggc
     2641 agcggtgatc cccgacctgg cgacggccct taccgggtcg gggcgggccg tgctggtggc
     2701 gccaccgggc accggcaaga cgacgcgagt gccgctcgcc ctgctggacg cgccgtggct
     2761 cgacgggcgg atcctgatgc tcgaaccgcg acgtgtcgcc gcccgcgccg ccgccaccta
     2821 catggccggg ctgctcggcg aagcggtcgg ggcgaccgtc ggctaccgga cccgcggaga
     2881 ctcgaaggtc ggtcgggcga cgcgcgtcga ggtggtgacc gagcggttgc tcacccgtcg
     2941 cctgcaacag gacccgtcgc tcgagggcat cgggctcgtg atcttcgacg aggtccacga
     3001 acggagcctc gacaccgatc tcgggctcgc gctgtgcctc gacgtcgccg gaagcctgcg
     3061 cgacgacctc cgcgtcctgg cgatgtcggc cacccccgac accgaggcca tcgccggcct
     3121 gctcggcgac gaatccggcc cggcgccgat catcgagagt tccggcgtcg cccaccccgt
     3181 cgacatcgtc tacctgggcc tggatcgtcg ccggcgtctc gacgacgagg tcgcggacgc
     3241 ggtcggccga gcccttcgcg aggcgcccga cggcgacgtg ctcgtcttcc tccccggacg
     3301 ccgggagatc cgctggaccg ccgagcggct cgcagcgcgc agcatcgaga cggtgccgtt
     3361 gacggccggt ctcgacccct catcgctcga cggcgcgctc cgcccgcgcc ctgacgggcg
     3421 gcgaaaggtc atcctcgcca cgtcggtggc acaaaccagc ctcaccatcg acggcgtgcg
     3481 ggtggtggtc gatgccggcc tcgagcggtc ggcgcgtttc gatccggccc ggggaatgtc
     3541 ggggctggtg accgtgcccg tctcccgagc ggcggccgac cagcgcgccg ggcgggcgag
     3601 ccgtcaggcc ccgggccgct gttaccggtt gtggagcgaa cccgagcagg cgtcgcggcg
     3661 ggcccagtcg gaaccggagc tggccgtcgc cgacctctgc gcgttggtgc tcgacgtggc
     3721 cgcatggggc gtaaccgacc cgaccgggct ccggtgggtc actcccccac cggtcgccgg
     3781 ttgggccgtc gccgccgcca cgttgcgcgc cgtcggggcg gtcggtgccg acggccggat
     3841 caccgccgac gggcggatga tgtccgatct cggcgcgcac ccccggctcg gccgcctgat
     3901 gatcgacgca gcccgtgtcg gcgacgacga cacgggcacg ctcctcgctg cactcatcga
     3961 ggccggcgtg cgcggcgacc tgcggcgagc gctcgaccga cccgaccgcg aggtcgaacg
     4021 ggtcgcggag cggtggcggc ggagcctccg gagcctgcgg gcggacggac ccgtcggcgc
     4081 gcgcggtgac gattccaccc cggttcccgc cccggcgccg ggcggcgacg aggccgtcgg
     4141 tgcgctcgtg gcggcggcgt tccccgaccg ggtgggccgg gcccgtgacg gcagccgaac
     4201 ccgctacctc ctcgcatccg gcgtcggcgc gtcggtcccg gagggctctc cgcttgccgg
     4261 tcacgactgg ctcg
//
LOCUS       DAOSOA010000293         4271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20711_length_4271_cov_5.252846, whole genome shotgun sequence.
ACCESSION   DAOSOA010000293 DAOSOA010000000
VERSION     DAOSOA010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20711_length_4271_cov_5.252846"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKY13_14330"
     CDS             complement(<1..590)
                     /locus_tag="PKY13_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT81168.1"
                     /translation="MTKLAGAHVIVTGGSEGIGLATAQLAARRGATVSLIARRPDVLA
                     EAAASIGGPVGTAAADVTDAAALATAIDGLVAAQGPCDVLVCCAGYALPGYFDDLPVE
                     EFDRHMQVNYLGAVHAVRAVLPSMRARRRGHVIVTSSTAGIIGVFGYGAYSPTKFAIR
                     GLAEVLRAETVGDGIGVGIVYPPDTKTPGFDRENRT"
     gene            complement(587..1486)
                     /locus_tag="PKY13_14335"
     CDS             complement(587..1486)
                     /locus_tag="PKY13_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81169.1"
                     /translation="MSNDVSADRPGESDPAERSRWRERAQHILANRAISVPTLTVRRF
                     FAISGIRQANLLAFNLFICVVPLTILLFAAIAPTRRHLDLGQVMVEQFHLHGVAADTM
                     RDAFPPNSGILGVASFIVVVTFAVSGFDVGSIFERTFAEAWGVAPYGGWRGALRGGFW
                     FVLVFGTFGLSQFLQGIPARHGRPLYLAVIPVILVMNYLFWLITPRLLLDKDLDPPDL
                     RPGAIMGMIASTVLWRLSAEVLPGWFDWYARGFGGVGVALAMLSWTYVVAIVWVVIVV
                     ASSAYWERTATVEEVLEATEPRS"
     gene            1525..2154
                     /locus_tag="PKY13_14340"
     CDS             1525..2154
                     /locus_tag="PKY13_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT81170.1"
                     /translation="MADGVWQMVADQRRQTVALVESLTEEQLSLPSLCEGWAVRDVAG
                     HVAWVALATPPEVVREVLLAGGKVHRAIDVQAKTYGSLGTDVLVEKLRLAVDSRIVTP
                     TITAESLASDTLVHNQDIRRPLGLAAAIDPALLRLALDHYLGANRFTGGKKRVKGLRL
                     VATDLDWSLGSGPEVRGPGEALLLAAVGRGAALADLDGDGLQVLQQRIA"
     gene            complement(2213..3928)
                     /locus_tag="PKY13_14345"
     CDS             complement(2213..3928)
                     /locus_tag="PKY13_14345"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HOT81171.1"
                     /translation="METVRSAPVRAATTTRNLFWGTGPGDHALTVTDGHWPTDMDGRV
                     FIVGPDKRRPGGHWFDQQGLLCRIDLRPGSDGRIDVRHRRVDTPLSRIRSRLPFLFTT
                     LSFMEISPFGLTNLANTNVQTIDDRLFVGYDAGRPLEVDPETMAVITPVGGNDEWYQA
                     SPGILEPGISVAAHPASDVDEGCLWFVNYSPLPLTPDTFVARWRLDGPVERWRLEGMT
                     EFDSIHDVKVTRDHIVFTDLPFIVEPATFAGKPQTTPNRDWTSLWIVAKSDLAATPVG
                     GSVRPLEIRIPMPTAHLSVDYEHPADRLTVYLEQIPIQDLMIRLRRGEYSHGTGAPND
                     PNFDGMVSLAPQPGVVGRYEIDLTTSEVVRSDLAWDDRFWGAVLATKDESSPAARAHQ
                     GQLWYSGIGFDPALVAQTWWDLYADAGLAAVVDPREVPMEPVPAGLARFDRESMKVAE
                     VYRFDEGSFPHPPTFVPRSGATDPDDGYIVTVIHRDGPKEVWVFDASHLEAGPLARAS
                     APEFNPPLLLHSCWMPPVPANRRRPDYRIPLWRDIRAAVTALPGLIVRIMRMGKRLHG
                     LGHLR"
BASE COUNT          659 a         1399 c         1600 g          613 t
ORIGIN      
        1 ttcgtgcggt tctcgcggtc gaaccccggc gtcttcgtgt cggggggata gacgatgccg
       61 accccgatgc cgtcgcccac cgtctcggcc ctgagcactt cggcgaggcc acggatcgcg
      121 aacttcgtgg gcgagtaggc gccgtatccg aagacgccga tgatgcccgc ggtcgacgag
      181 gtgacgatca cgtggccgcg ccggcgggct cgcatcgagg ggaggacggc gcggacggca
      241 tggacggcgc cgaggtagtt cacctgcatg tgacgatcga actcctcgac cggtaggtcg
      301 tcgaagtatc ccggcagtgc gtagccggcg cagcagacca gcacgtcaca cggcccctgg
      361 gcggcgacga gtccgtcgat cgcggtggcc aacgccgcag cgtcggtgac gtccgcggcc
      421 gccgtgccca ccggcccgcc gatcgaggcc gccgcctccg ccagcacgtc gggacgtcgg
      481 gcgatcaacg acaccgttgc accgcggcga gccgcgagct gcgccgtggc gaggccgatg
      541 ccctcggacc cgccggtcac gatcacgtgc gcgcccgcga gcttcgtcac gagcgcggct
      601 cggtggcctc gaggacttct tcgaccgtcg cggtccgctc ccagtaggcc gacgacgcga
      661 cgacgatcac gacccacacg atggcgacca cgtacgtcca gctgagcatc gcgagtgcga
      721 caccgacgcc gccgaacccg cgggcgtacc agtcgaacca gcctggcagc acctcggcgc
      781 tgagccgcca caggacggtg ctcgcgatca tgcccatgat cgcccccggg cgaaggtcgg
      841 gtggatcgag gtccttgtcg agcaggagcc gcggtgtgat cagccagaac aggtagttca
      901 tgacgaggat caccgggatc acggcgaggt agaggggccg gccatgccgg gcggggatcc
      961 cctgcaggaa ctggctcagt ccgaaggtgc cgaacacgag cacgaaccag aagccgccac
     1021 ggagcgcccc gcgccagccg ccgtacggcg cgacgcccca ggcctcggcg aacgtccgct
     1081 cgaagatcga cccgacgtcg aacccgctga cggcgaacgt cacgacgacg atgaacgagg
     1141 cgacccccaa gatgccggag ttgggcggga aggcgtcgcg catggtgtcg gccgcgacgc
     1201 cgtggagatg gaactgttcg accatcacct gcccgaggtc gaggtggcga cgtgtcggtg
     1261 cgatcgcggc gaacagcagg atcgtgagcg gcaccacaca gatgaagaga ttgaaggcga
     1321 ggaggttcgc ctggcggatg ccgctgatcg cgaagaaccg gcgcacggtg agggtcggca
     1381 cgctgatcgc ccggttggcg aggatgtgtt gggcgcgttc acgccagcgt gaccgctcgg
     1441 cggggtcgct ctcgccaggg cggtcggcgc tgacgtcgtt cgacatacag cgagtctccc
     1501 accgccggat ggcagactcg tgcgatggca gatggcgtgt ggcagatggt ggcggaccaa
     1561 cgccgacaga ccgtggcgtt ggtcgaatcg ctgaccgagg agcagttgtc gctcccgtcg
     1621 ctgtgtgagg gctgggcggt gcgcgacgtc gccggtcacg tcgcctgggt cgcgctcgcc
     1681 acaccgcccg aggtggtgcg cgaggtgctc ctggccgggg gcaaggtgca ccgcgccatc
     1741 gacgtccagg cgaagacgta cggctcgctc ggcacggacg tgctcgtcga gaagttgcgt
     1801 ctcgccgtcg acagccggat cgtgaccccg acgatcactg ccgagtcgct ggcgtcggac
     1861 acgctggtgc acaaccagga catccgccgt ccgttggggc tcgcggcggc gatcgatccg
     1921 gcgttgctgc gcctcgcgct cgaccactac ctgggtgcga accggttcac cggtggcaag
     1981 aagcgcgtca aggggttgcg gctcgtggcg accgacctcg actggagtct cgggtcgggc
     2041 cccgaggttc gcgggcccgg cgaggccctg ttgctcgccg cggtcggccg cggcgccgcc
     2101 ctcgccgacc tcgacggcga cggtctccag gtgctccagc agcgaatcgc ctgagtccgt
     2161 gactcggatc gccgaggtac cggagcgtta gcagggagcg aagcgaccgt gcctagcgaa
     2221 ggtggccgag gccatggagg cgcttgccca tccgcatgat gcgcacgatg agcccgggca
     2281 gggcggtcac ggcggcgcgg atgtcgcgcc acaacgggat gcggtagtcg ggccgacggc
     2341 ggttcgccgg caccggcggc atccagcacg agtgcaggag taacggcggg ttgaactcgg
     2401 gtgcggacgc ccgggccagg ggcccggcct cgagatggga cgcgtcgaac acccacacct
     2461 ccttggggcc gtcgcggtgg atcacggtca cgatgtaccc gtcgtcgggg tcggtggcgc
     2521 cggaacgggg cacgaaggtg ggcggatggg ggaaggagcc ctcgtcgaag cggtagacct
     2581 cggccacctt catcgactcg cggtcgaagc gggcgaggcc ggcgggcacg ggctccatcg
     2641 gtacctcgcg gggatcgacg acggcggcga gcccggcgtc cgcgtacagg tcccaccacg
     2701 tctgggcgac cagcgccgga tcgaacccga tgccggagta ccagagctga ccctggtggg
     2761 cccgcgccgc cggcgacgac tcgtccttgg tcgccaggac ggcaccccag aagcggtcgt
     2821 cccaggcgag gtcggatcgc acgacctcgc tcgtcgtgag gtcgatctcg tagcggccga
     2881 cgaccccggg ctgcggggcg aggctgacca tcccgtcgaa gttcggatcg ttgggcgccc
     2941 cggtgccgtg ggagtactcg ccgcgacgga ggcggatcat caggtcctgg atcgggatct
     3001 gctcgaggta caccgtgagc cggtcggcgg ggtgctcgta gtcgaccgac aggtgcgccg
     3061 tcggcatcgg gatacggatc tcgagggggc gcaccgaccc accgaccggc gtcgccgcga
     3121 ggtcggactt cgcgacgatc cacaggctcg tccagtcgcg gttcggtgtg gtctgcggct
     3181 tgccggcgaa cgtggccggc tcgacgatga acggcaggtc ggtgaacacg atgtggtcgc
     3241 gggtgacctt cacgtcgtgg atcgagtcga attccgtcat gccctcgagc cgccagcgct
     3301 cgacggggcc gtcgaggcgc caccgggcca cgaacgtgtc gggcgtgagc ggcagcgggg
     3361 agtagttgac gaaccagagg cagccctcgt cgacgtcgga tgccgggtgt gcagcgaccg
     3421 agatgccggg ttcgaggatg cccggcgacg cctggtacca ctcgtcgttg ccgccgaccg
     3481 gggtgatgac ggccatggtc tcggggtcga cctcgagggg ccgaccggcg tcgtagccga
     3541 cgaacagccg gtcgtcgatg gtctgcacgt tggtgttggc gaggttggtc aatccgaacg
     3601 gtgagatctc catgaaggag agggtcgtga acaggaacgg cagccgggac cggatcctcg
     3661 acagcggggt gtcgacgcgg cggtgccgca cgtcgatgcg gccgtcgctt ccgggacgga
     3721 ggtcgatgcg gcacaggaga ccctgctggt cgaaccagtg gccgccgggg cggcgcttgt
     3781 cggggccgac gatgaacacg cggccgtcca tgtcggtcgg ccagtggccg tcggtgacgg
     3841 tcagcgcgtg gtcgccggga cccgtgcccc agaacaggtt gcgcgtcgtg gtcgctgctc
     3901 gcaccggcgc ggaacggacg gtctccatcg gatctccccc tgactgtcgg cgcgtgcccc
     3961 ccgccgacgc cgcaacgtta acggcgttca tctgccgacg cacgggccag acgccgggcg
     4021 acctcggagc gccccgcaga agcagcccga gccgtcgccg ggggagcgcg gcgtgaaatc
     4081 cgaccttttg gcgggccgca ggtggggacg gcggcggtcg cctcctagcc tgacggccat
     4141 gcgtcgctgg gtggtcgcca cggtctcggt tgtctcgtcg atggtgttga cgagtgtgat
     4201 gacgggcctg gtcaccctcg gcggtgccgt tgccccggcg tcggcacaga cgtcgttcac
     4261 gttcaatggc g
//
LOCUS       DAOSOA010000294         4251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20848_length_4251_cov_6.612488, whole genome shotgun sequence.
ACCESSION   DAOSOA010000294 DAOSOA010000000
VERSION     DAOSOA010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20848_length_4251_cov_6.612488"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKY13_14350"
     CDS             <1..548
                     /locus_tag="PKY13_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81172.1"
                     /translation="GVDSLFPGQRLHVPWSEARIQVHVGDRCHEFVARYEATNRSSGA
                     ESTIPSDGEHPDATAVPVRVDRSSGYFRALVALCEPQLLDPSSSDVATDLQIARRLNR
                     SGRESGRLSGKTVERRLDNCRTRFGLKVTDENGFSVGLERRDSRRLLVEVALLTATVT
                     PEDLRVLGDEEPDREAADAGR"
     gene            535..>4251
                     /locus_tag="PKY13_14355"
     CDS             535..>4251
                     /locus_tag="PKY13_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HOT81173.1"
                     /translation="MPVGDHGDDVVTWRVRVASRASNSGDHEIVETEVGDLVVEIGPW
                     ARVGDLVIELAGSHGLCDDPADIGWAGLELADGSMAAADERLCTLPVAHGDRLVVVVG
                     IGDGDAPAPAAAFGAAGSPSVRYGLMPVGEHDGGPLVELEVGLVPIGRNETDNIVVLD
                     DPSVSRRHAHLIVGNEVVVTDVGSTNGTAIDGVPVSGERRLPVGSRLELGDVSFELRA
                     IAPIVVDPSSDDSRPPSGRPTPQRGIVVFNREPSVPEPIDEVTITLPEPPSAPEEHRM
                     PWLSIAIPLVLAAAMVAVPLIVGGLDRSYVLLSAGFLLMSPIMVLASFWETRRVAARR
                     RRVGLADFHERVGSAERRGRAAVDDERRRREVAHPGPAARRRQSRLAETGLWAGAERF
                     DEQVRIGVGRIPTLVGFRRDGGGAPWPENQPPLGLDGEACAELERVAASVRHHDAAPV
                     TIPASGIVAVVGERDWADAVVRAIVLQLATSLPLTAVRFTFVTDELDAFDWAKWLPHD
                     IGAGTVVTRSDAGIRHVVVVDAADGERLLDWAELAADAARSGAFVVWAGRHRADVPTT
                     TSTLVEAVGTGSLVGGPPVADVVDLVSGDVVRSVSVDLVGGSDADRWARDLAPLRDPV
                     GGGDPGRLPAAVPIGDVLLDSAALFDPDVVVEAWAQPAPLIARLGRTAGGIATVDLRR
                     DGPHAVIAGTTGAGKSELLQTMVVSLALSLPPSRVNFLLVDYKGGAAFAECAALPHTV
                     GLVTDLDGNLVRRVLTSLRAEIRRREAILAAAGAKDLETLEEREPAGAPPSLVIVVDE
                     FASLTTEVPGFVEDMIDLARRGRSLGLHLVLATQRPAGVVSDHLLANANLRIALRVAD
                     LHESTDLVGVPDAAAIARTRPGRAVARLGPGDVLHLQTAFCGGRTPAPDGRDRSRDVV
                     VHDFGIEPTGDGGAPAASVPDDAPTDLCRLVEIARAAAGRSGEPAPRRPWLEPLPGRV
                     EMSTRLAGGPDGRVTAVVGVADDPDRQSQFVMRYDLGAGPLAVYGAGDAPVRFVSAMV
                     AGLVNDRSPADLQIDVIDGESGALGRLASLPHVGSVVDAADSDRISRLLRQLSTSIAR
                     RGERGPTRLIVVNDLAGFAGRSERLDGGAAIEALARIAADGPAAGVLVITLAGRRVGV
                     PPVLAASAGRRVVMAMTTDDDVVSLGVDPSLLTVDAPGRAVVDGLAVQTVDLPVSGEW
                     SLEAIAARAVARWGELRAPSVGVLPERIEASAVGA"
BASE COUNT          512 a         1464 c         1599 g          676 t
ORIGIN      
        1 tcggggtcga cagtctcttc cccgggcaac ggctccacgt tccgtggtcc gaggcccgga
       61 tccaggtgca cgtcggcgat cggtgtcacg agttcgtcgc ccgctacgag gcgaccaacc
      121 gatcgtcggg ggcggagtcg acgattccct ccgacggcga gcaccccgac gccaccgccg
      181 tgcccgttcg cgtcgaccgc tcctcgggct acttccgcgc gttggtcgcg ttgtgtgagc
      241 cgcagctgct cgacccgtcg tcgtccgacg tggccaccga cctccagatc gcccggcggc
      301 tgaaccggtc gggtcgcgag tcggggcggc tcagtggcaa gaccgtcgaa cgtcgtctgg
      361 acaactgtcg gacgaggttc ggcctcaagg tgaccgacga gaacgggttc tcggtcggct
      421 tggagcgacg ggactctcgg cggctgctgg tggaagttgc gctgctcacg gcgaccgtga
      481 cgcccgagga tctccgggtg ctcggtgatg aggagcccga tcgcgaggcg gccgatgccg
      541 gtcggtgacc acggcgacga cgtcgtcacg tggagggtgc gcgtggcgtc gcgggcgtcc
      601 aactccgggg accacgagat cgtcgagacc gaggtcggtg acctcgtcgt cgagatcggg
      661 ccctgggctc gggtcggtga cctcgtcatc gagcttgccg ggtcgcatgg tctgtgcgat
      721 gacccggccg acatcgggtg ggccgggttg gagctggccg acggctcgat ggccgcggcc
      781 gacgagcggt tgtgcacgct gccggtcgca cacggcgatc gtctggtggt cgtcgtcggg
      841 atcggggatg gcgacgcccc ggcaccggcc gcagcgttcg gcgcggccgg ttcgccatcg
      901 gttcgctacg gactgatgcc cgttggcgag cacgatggcg gtccgttggt ggagctcgaa
      961 gtcggcctcg tccccatcgg gcggaacgag accgacaaca tcgtggtgct cgatgacccg
     1021 tcggtctccc gtcgacatgc ccacctgatc gtcggcaacg aggtggtcgt caccgatgtc
     1081 ggctccacga acggcaccgc gatcgacggc gtgccggtgt cgggggagcg ccgtctcccg
     1141 gtgggatcgc gtctggagct gggcgacgtg tcgttcgagc tccgggccat cgctccgatc
     1201 gtggtggacc catcgtcgga cgactcacga ccgccgtcgg ggcgtccgac gccccagcgc
     1261 ggcatcgtcg tgttcaaccg tgagccatcg gttccggaac cgatcgacga ggtgacgatc
     1321 acgctgcccg agccgccgtc ggcccccgag gagcaccgga tgccgtggct gtcgatcgcg
     1381 atcccgctgg tgctggcggc cgcgatggtc gcggtgccgc tgatcgtcgg cgggctcgac
     1441 cgctcgtacg tcctgttgtc ggcgggcttc ctgctcatga gcccgatcat ggtcctggca
     1501 tcgttctggg agacgcgacg cgtggcggcg cgccgccgcc gggtcggact cgccgacttc
     1561 cacgagcgcg tcggatcggc ggagcgtcgc gggcgggccg cggtcgacga cgagcgccgc
     1621 cgacgcgagg tcgcgcatcc gggtccggcg gcccgtcggc ggcagtcccg gctggccgag
     1681 accggcctgt gggccggggc ggaacggttc gacgagcagg tgcgaatcgg agtcggccgg
     1741 atcccgacgc tcgtcgggtt ccgacgtgac ggcgggggag cgccgtggcc ggagaaccag
     1801 ccgccactcg gcctcgacgg cgaagcgtgc gctgagctcg aacgggtcgc cgcttcggtc
     1861 cgacatcacg acgctgcccc ggtcacgatc ccggcgtcgg gcatcgttgc cgtggtcggt
     1921 gagcgcgact gggccgatgc tgtggtccgg gcgatcgtcc tgcagctcgc cacgtcactt
     1981 ccactgaccg ccgtccggtt cacgttcgtc accgacgagc tcgatgcctt cgactgggcg
     2041 aagtggttac cgcacgacat cggggcgggc accgtggtca cccggtcgga cgccgggatc
     2101 cgacacgtcg ttgtggtcga cgccgccgac ggggaacgtc tgttggactg ggccgaactg
     2161 gcggccgatg ccgcccgcag cggagcgttc gtcgtctggg ccggccgtca tcgcgccgac
     2221 gtcccgacga cgacgtccac cctcgtcgag gcggtcggga ccggcagcct cgtcggcggg
     2281 ccgccggttg cggacgtcgt cgacctggtc agtggcgatg tggttcgatc cgtctcggtc
     2341 gacctcgtcg gcggctccga cgccgaccga tgggctcgcg acctggcgcc gttgcgcgat
     2401 ccggtcggcg gcggtgatcc gggccggctc ccggcggccg tgcccatcgg cgacgtgctc
     2461 ctggacagcg ctgcactgtt cgatcccgac gtcgtggtcg aggcgtgggc ccagccagct
     2521 ccgctgattg cgcgcctggg ccgaacggcg ggcgggattg ccacggtgga cctgcgccgc
     2581 gacggcccgc acgccgtgat cgccggcacg acgggcgcgg gcaagagcga gttgttgcag
     2641 acgatggtcg tgtcgctcgc cctgtccctc ccgccgtcgc gggtgaactt cctgctcgtg
     2701 gactacaagg gcggcgccgc gttcgccgag tgcgccgcgc tgccacacac cgtcgggctc
     2761 gtcaccgacc tcgacggcaa cctcgtgcgc cgggtcctga cgtcgctccg cgccgagatc
     2821 cgacgccgag aggcgatcct cgctgcggcg ggcgccaagg atctcgagac gctcgaggag
     2881 cgtgagccgg cgggggcgcc gccgagcctc gtgatcgtcg tcgacgagtt cgcgtccctg
     2941 acgaccgagg tgcccgggtt cgtggaggac atgatcgacc tcgcgcgacg cggtcgctcg
     3001 ctcggtctgc acctcgtcct ggcgacgcaa cgtcccgcag gtgtggtgag cgaccatctc
     3061 ctggccaacg cgaacctccg gatcgcgctg cgggtcgccg acctgcacga gagcacggac
     3121 ctggtggggg tgcccgatgc cgccgccatc gctcgcaccc ggcccgggcg cgccgtggct
     3181 cgcctcgggc cgggcgacgt cctccacctg cagacggcgt tctgtggtgg gcgcactccg
     3241 gcgcccgacg ggcgcgaccg atcccgtgac gttgtcgtcc acgacttcgg gatcgagccg
     3301 acgggtgacg gcggcgcacc cgcggcgtcg gtgcccgacg atgctccgac cgacctgtgt
     3361 cgactcgtcg agatcgcgag ggctgcggcc ggccgctccg gtgagccggc acctcgtcga
     3421 ccgtggctcg aaccgttgcc ggggcgcgtc gagatgtcga cgcgcctcgc aggtggcccc
     3481 gatgggcggg tcacggcggt ggtgggtgtc gccgacgatc ccgaccgtca gtcgcagttc
     3541 gtgatgcgat acgacctcgg tgcggggccg ctggccgtgt atggcgccgg cgatgctccc
     3601 gtccggttcg tctcggccat ggtggccggc ctcgtgaacg accggtcgcc cgccgacctg
     3661 cagatcgacg tgatcgacgg cgagtcgggc gcgctcgggc gactggcgtc gctgccccac
     3721 gtcggctcgg tggtggacgc tgccgacagc gatcggatct cccggttgtt gcggcagctg
     3781 agcacctcga tcgctcgtcg gggcgagcgc ggacccacac ggttgatcgt ggtgaacgac
     3841 ctcgccgggt tcgccgggcg ctccgagcgg ctcgacggcg gggcggcgat cgaagcgctt
     3901 gcccgcatcg ccgccgacgg accggctgcc ggcgtgctcg tgatcacctt ggcagggcgc
     3961 cgggtgggtg tcccgccggt actggcggcg tcggcgggac ggcgggtggt gatggcgatg
     4021 acgaccgacg acgatgtcgt gtcgctcggg gtcgacccct cgttgctgac ggtcgacgcc
     4081 cccggccgcg ccgtcgtcga cggcctcgcc gtccaaacag tcgacctccc ggtctcggga
     4141 gagtggtcac tcgaggcgat agctgcccga gcggtcgccc gctggggcga gctgcgcgcc
     4201 ccgtcggtcg gagtgcttcc agagcggatc gaggcatcgg cggtcggcgc g
//
LOCUS       DAOSOA010000295         4237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_20953_length_4237_cov_5.435916, whole genome shotgun sequence.
ACCESSION   DAOSOA010000295 DAOSOA010000000
VERSION     DAOSOA010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20953_length_4237_cov_5.435916"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKY13_14360"
     CDS             <1..299
                     /locus_tag="PKY13_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81174.1"
                     /translation="VSWFIERRLKGIGQKLARAREDLAIADEQLVYLADDAADAQVRS
                     VVSEGGSAGLDYNEAQRHSDAMARHRSDLVDTIARLEATQDELLDQLVAKRSGS"
     gene            296..898
                     /locus_tag="PKY13_14365"
     CDS             296..898
                     /locus_tag="PKY13_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT81175.1"
                     /translation="MSDVTAGSAPPLRIVVAEDDAIIRMDLCLILQEEGYDVVGDCGQ
                     GDLAVELVREHRPDVAILDVKMPGLDGISAARTISGERLCAVVLLTAFSQRKLIESAR
                     DAGVMAYLVKPFQREELVPAVELAFGRFREMAALAEHTSSLEEQLAVRKLSDRAKARL
                     IADHGLTEDEAFRFLQRGSMDRRVPLRQVAEGVLDGSIVP"
     gene            945..3623
                     /gene="polA"
                     /locus_tag="PKY13_14370"
     CDS             945..3623
                     /gene="polA"
                     /locus_tag="PKY13_14370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOT81176.1"
                     /translation="MPTLMLIDGNSLTYRAFFALPSDMATASGQVTNAVFGFTSMLIN
                     LKRDHQPDLIAVTFDRPEPTFRHEMVPTYKANREASPDILRQQMGLVRQLVETLQIPI
                     IDQAGIEADDIIATLATQAEAEDISTIIVTGDRDSYQLVHDPHIKVLYNKRGVSDYAF
                     YDEAGIFERTGVRPDQYVFYAALRGDPSDNLPGVAGVGEKTAAKLINTYGDLDGIFAN
                     VDAQTPKLRSNLIEHEAQARSNVAMMTLRRDVELPLGPSDLVQGQFDNDAVFDLFKFL
                     EFGSLLGRFGEAFGGDSSASLEPARNVLEAEVTEIDDPAEAESLLSSLAAGAAPLAIA
                     VGWEGAPGRSAMSGLAIVTDASLGAVSWIPANLLAGATPAFDALLADGPAIVAHDAKA
                     LLRRLLDEGRPLPRLHADVKIAAYLLDPADTRYSVAELLHRYTEFELAQGGATETGQL
                     DFDGATESIAAEPAREALGVAFVVEPITAALDSQGLAKLAHEMEYPLIAALARMEHLG
                     VGVDRAELERLNEQLKSECERLRALVIEEAGEEFNVNSTQQLGPILFEKLGLPVLKKT
                     KTGKPMTDAGVLEKLRDQHPIVDHLLAYREVEKLRGTYGSGLLAEIQPDGRIHATFNQ
                     TVARTGRLSSDAPNLHNIPVRSEAGRQFRKAFIPSPGSVFLIADYNQIELRCIAHLAE
                     DPGLIEAFTGGHDIHNETASRVFNVEPGAVTLEMRSKAKMVSYGLAYGMEAYGLGQRL
                     SIPTREAQEILDAYFHAFPSVKAYMDRTVEEARERGYTETLFGRRRRIPELASGQRNV
                     RMAGERQAMNAGIQGLAADIFKVALVRIDTAFQAADHQAELILQVHDEVICEVVPEHL
                     DEVRTQVVDIMTGAFEMNVPLEVNVSSGDTWAAAKG"
     gene            complement(3644..>4237)
                     /locus_tag="PKY13_14375"
     CDS             complement(3644..>4237)
                     /locus_tag="PKY13_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81177.1"
                     /translation="TATEPNERYRLTSGETELDTATSEGQLTVLVGHLEGVPPRTLPE
                     PMTVERLDADGHVVGRITAEDVQPDTIPGCESVNQAVELVPSSTPADSATADAPVRDA
                     TRTIEASSVALTADQSIDPIDVYWTSPTSAWVKYQVVYFAATPENGNTAQGPYLWAEV
                     TFDGASWQPTAEARCAYAKSRGTNDPCTDVPPVVPMR"
BASE COUNT          667 a         1495 c         1417 g          658 t
ORIGIN      
        1 gcgtgtcgtg gttcatcgaa cgccgcctga agggcatcgg ccagaagctc gcccgggccc
       61 gtgaagacct ggcgatcgcg gacgagcagc tcgtgtacct cgccgatgac gccgccgacg
      121 cccaggtgcg ttcggtggtg tccgagggcg ggtccgccgg gctcgactac aacgaggcgc
      181 aacgccacag cgacgccatg gcccggcacc gtagcgacct tgtcgacacg atcgcccgac
      241 tcgaggccac ccaggacgag ctcctcgacc agctggtcgc caagcggtcc ggatcgtgag
      301 cgacgtcacc gccggcagcg cgcccccgct gcggatcgtc gtcgccgagg acgacgcgat
      361 catccgcatg gacctctgcc tgatcctcca ggaggagggg tacgacgtgg tcggcgactg
      421 cgggcagggc gacctcgccg tcgagctcgt ccgcgagcac cgtcccgacg tcgcgatcct
      481 cgacgtgaag atgccgggcc tcgacggcat ctccgccgct cgcaccatct cgggcgagcg
      541 gctgtgcgcc gtcgtcctgc tcacggcgtt cagccagcgc aagctcatcg aatcggcccg
      601 tgacgccggg gtgatggcct acctggtgaa gccgttccag cgcgaagaac tcgtgcctgc
      661 cgtcgagctc gccttcggtc ggttccgcga gatggcggcg ttggccgagc acacgtcgtc
      721 gctcgaggag cagttggcgg tccgcaagct ctccgaccgt gccaaggccc gcctgatcgc
      781 cgatcacggg ctgaccgagg acgaggcgtt ccgcttcctc caacggggtt cgatggaccg
      841 gcgggtgccg ctccgccagg tcgccgaggg tgtcctcgac ggcagcatcg tcccgtaggc
      901 gggcccgacc ccgctgtcag gggtgtcgat taggtttggc cccgatgccg accctgatgc
      961 tcatcgatgg caactcgctc acgtaccggg cgttcttcgc gctcccgtcc gacatggcca
     1021 cggcgtcggg gcaggtcacc aacgccgtgt tcgggttcac gtcgatgctc atcaacctca
     1081 agcgcgacca ccagcccgac ctgatcgcgg tcacgttcga tcgccccgag ccgacgttcc
     1141 gccacgagat ggtgcccacc tacaaggcca accgcgaggc gagccccgac atcctccgcc
     1201 agcagatggg gctcgtgcgc cagttggtcg agacgttgca gatcccgatc atcgaccagg
     1261 ccggcatcga ggccgacgac atcatcgcga cgttggccac ccaggccgag gccgaggaca
     1321 tctcgaccat catcgtcacg ggcgaccgcg acagctacca gctggtccac gacccgcaca
     1381 tcaaggtcct ctacaacaag cgcggcgtca gcgactacgc gttctacgac gaggccggca
     1441 tcttcgagcg caccggcgta cggcccgacc agtacgtgtt ctacgcggcg ctccgcggtg
     1501 atccgtccga caacctgccc ggcgtcgccg gcgtgggcga gaagacggcc gccaagctga
     1561 tcaacaccta cggcgacctc gacggcatct tcgccaacgt cgacgcacag acgcccaagc
     1621 tgcggtccaa cctcatcgag cacgaggcgc aggcccgctc caacgtggcc atgatgacgc
     1681 tgcgccgcga cgtcgagctg cccctcggcc cgtccgacct cgtgcagggt cagttcgaca
     1741 acgacgccgt ctttgacctg ttcaagttcc tcgagttcgg atcactgctc ggccgattcg
     1801 gcgaggcctt cgggggcgac agttcggcat cgctcgagcc ggcccgcaac gtgctcgaag
     1861 ccgaggtcac cgagatcgac gacccggccg aggccgagtc gctgctctcg tcgctcgccg
     1921 ccggtgcggc gccgttggcg atcgcggtcg ggtgggaggg tgcgcccggc cgatccgcga
     1981 tgtcgggcct ggccatcgtg accgacgcgt cgctcggggc ggtgtcgtgg attcccgcca
     2041 acctgctcgc tggggccacc ccggcgttcg acgcgctgct cgccgacggt ccggcgatcg
     2101 tcgcccacga cgccaaggcg ttgttgcgcc gtctgctcga cgagggccgg ccgctcccgc
     2161 gactgcacgc cgacgtcaag atcgccgcgt acctcctcga ccccgccgat acgcggtaca
     2221 gcgtggccga gctgctccac cgctacaccg agttcgaact cgcgcagggt ggtgccaccg
     2281 agaccggcca gctcgacttc gacggtgcca ccgagtcgat cgccgccgaa ccggcacgcg
     2341 aggcccttgg ggtggcgttc gtcgtcgagc ccatcaccgc ggcgctcgac tcccaaggcc
     2401 tcgccaaact ggcccacgag atggagtacc cgctcatcgc ggcgctcgcc cgcatggagc
     2461 acctcggcgt cggcgtcgac cgcgccgagc tcgagcggct caacgaacaa ctcaagtcgg
     2521 agtgcgagcg gctccgcgcc ctcgtcatcg aggaggcggg cgaggagttc aacgtcaact
     2581 ccacccagca gctcgggccg atcctgttcg agaagctcgg tctgcccgtc ctcaagaaga
     2641 ccaagaccgg caagccgatg accgatgccg gtgtgctgga gaagctgcgc gaccagcacc
     2701 ccatcgtcga ccacctgttg gcctaccgcg aggtcgagaa gctgcggggc acctacggct
     2761 ccggcctcct cgccgagatc cagcccgatg gccgcatcca cgccacgttc aaccagacgg
     2821 tcgcccgcac gggccggctc agctccgacg ctccgaacct ccacaacatc ccggtgcgtt
     2881 ccgaggcggg tcggcagttc cgcaaggcgt tcatcccgag cccgggctcg gtcttcctca
     2941 tcgccgacta caaccagatc gagcttcgct gcatcgccca cctggcggag gatcccggtc
     3001 tgatcgaggc gttcaccggc ggccacgaca tccacaacga gaccgcgtcg agggtgttca
     3061 acgtcgagcc gggcgccgtg acgctcgaga tgcgctcgaa ggcgaagatg gtgtcgtacg
     3121 gcctggccta cggcatggag gcgtacggcc tcggtcagcg cctgtcgatc ccgacgcgtg
     3181 aggcgcagga gatcctcgac gcctacttcc acgccttccc gtcggtgaag gcgtacatgg
     3241 accgcacggt cgaggaagcc cgcgagcgcg ggtacaccga gacgctgttc gggcgtcgcc
     3301 gtcgtatccc cgagctcgcg tcgggtcagc gaaacgtccg gatggcgggg gagcggcagg
     3361 ccatgaacgc cggcatccag ggtcttgccg ccgacatctt caaggtggcg ctggttcgca
     3421 tcgacacggc gttccaggcg gccgaccacc aggccgaact catcttgcag gtgcacgacg
     3481 aggtcatctg cgaggtcgtg cccgagcacc tcgacgaggt gcgcacccag gtcgtcgaca
     3541 tcatgaccgg tgcgttcgag atgaacgttc cgctcgaggt caacgtgtcc tcgggcgaca
     3601 cctgggccgc cgccaagggc tgatcaacac cgccacgggc tgatcagcgc atggggacga
     3661 ccggaggcac gtcggtgcac gggtcgttcg tgcctcggga cttggcgtag gcgcagcgtg
     3721 cctcggcggt cggttgccac gacgctccgt cgaaggtgac ctcggcccac aggtacgggc
     3781 cctgcgccgt gttcccgttc tccggagtcg cggcgaagta gacgacctgg tacttcaccc
     3841 aggccgaggt gggactcgtc cagtacacgt cgatcgggtc gatcgactgg tcggccgtca
     3901 gggcgaccga ggacgcctcg atggtcctcg tggcgtcgcg gacgggggcg tcggcggtgg
     3961 cggaatcggc cggggtcgac gacggaacca actcgacggc ctggttgacg gactcgcatc
     4021 cggggatggt gtcgggttgc acgtcctcgg ccgtgatgcg cccgaccacg tgtccgtcgg
     4081 cgtcgagccg ttcgaccgtc atgggttcgg gcagggttcg tggcgggaca ccctcgaggt
     4141 ggccgacgag gacggtgagt tggccctcgc tggttgccgt gtcgagctcg gtctcgccgc
     4201 tcgtgaggcg atagcgctca ttgggttcgg tggcggt
//
LOCUS       DAOSOA010000296         4204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21183_length_4204_cov_4.397927, whole genome shotgun sequence.
ACCESSION   DAOSOA010000296 DAOSOA010000000
VERSION     DAOSOA010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21183_length_4204_cov_4.397927"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PKY13_14380"
     CDS             <1..923
                     /locus_tag="PKY13_14380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT81178.1"
                     /translation="QLMAPTVTVAIPVLDEEAHLGACLDAIEAQTYSGIVEVLVIDGG
                     SSDRSVEIARQHQMVRVLDNPRRIQSAALNRALDEAAGEILVRVDGHCVIAPDYVERC
                     VAALDATGAAIVGGGMTPVANGAMQEAIAAAMGSRIGAGPARFHSSGDAGWVDTVYLG
                     AYRVADAVAVGGYAADMSINEDAEFAIRLASRGGVWFDPTIRSTYTPRSSLAAVARQF
                     YRYGRGRAVTVRRHPASLSPRQLAAPALVLGILSPWRRPVLGAYAAVVAGWSFAGRSD
                     RPARAGLVLPAMHVPWGVGFLTGLVLGGRS"
     gene            880..1866
                     /locus_tag="PKY13_14385"
     CDS             880..1866
                     /locus_tag="PKY13_14385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOT81179.1"
                     /translation="MSASSPVWFWAAAASLTGISVAVVIPAFEAAPVLDTTLASVVGQ
                     TLAPTEIVVVDDGSTDDTAAVAERWSEVAPVRVVRQRNQGPAAARRAGVAATTAPLLA
                     FVDADDVWLADHLDVAVAVHARSGGLVSPDAFSWRPGSALPSATRRRRFPIPAPHRQR
                     TGIVRENFVFSSVVVSRRDYEAAGGFRDGVTGAEDWDLWIRMVRRGTLVRGSDRPTWL
                     YRVGGVSLTDRPDMRATYEEVLRRVLADSDDAGERRAAEEHLGRLQARGQLDRAYAAA
                     RSGDPGVARRLARSALHGRPRVAAEALSVAVAPGLATRLGDEARARWTAGRR"
     gene            1863..3104
                     /locus_tag="PKY13_14390"
     CDS             1863..3104
                     /locus_tag="PKY13_14390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT81180.1"
                     /translation="MSRTHFHTDCYSFSGSETTLMLLLDAAARRGDEFTFTYRRWPEY
                     EHGLFRRLPAGVRARGLRLPDPADVKHAVTGGRPGTGARLVRGAITLLPFRQLFLAYD
                     TVRLTALLRKERPDVLHVNNGGLPGSISCNAAAIAGRLAGVPVVVMVVNNLAFAYNRP
                     GRWFDYPVDRLLVRSVTRFVTGSAAAGTALRRVLRLPEGQHVVIPNGVEQRLPTASAD
                     ETRSALGLSPGIAVVSVVARLEARKGHRHLLDALRLLPADRRAALRVLFAGAGPERGR
                     LEQEVTRLGLTDVVQFLGDHPDPWSLYEVSDLVVLPSVRHEDLPIVLIEAMAAGVPAV
                     ASRVAGTVELIEAGVTGLLVEPAEPRQLADAIAGLLDDPDRRRAMGKAATLRYEATYT
                     PAQAVAAYRDLTDRLLTGVTA"
     gene            3101..3637
                     /locus_tag="PKY13_14395"
     CDS             3101..3637
                     /locus_tag="PKY13_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81181.1"
                     /translation="MILDLGCGSSKRPGAVGVDIGPFPGVDVVHDLNRHPFPFEDGVA
                     DEIYLDNVLEHLDDVIATMEELHRIGRDGCRIRIDVPYFRSRWAAMDPTHVHAFTPES
                     FAYFDPTHPFSERYGYSAVRFDVESVVYNERFPARGPRALVARFANRRAERYESYLAP
                     IIPLDELTFTLRVRKHAR"
     gene            3634..>4204
                     /locus_tag="PKY13_14400"
     CDS             3634..>4204
                     /locus_tag="PKY13_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81182.1"
                     /translation="MTASRLRLGVYADLRYQRGAEGVTCDTAFVRFIAALGRHVDHLV
                     VFGRLGEVVSPMATLGTDEAEFVPLPDYRSLADVGAVSQAIAPSRRAWRRPLEALDAV
                     LVFGPHPVATALVRDARRRGVAVVLGVRQDLPGYLGLRASGRTRPVVLAAASGLEWGW
                     RRSARRQPVVVTGSDLARNYRNATWLLDVS"
BASE COUNT          532 a         1530 c         1470 g          672 t
ORIGIN      
        1 ctcagctgat ggcgccgacc gtcaccgtcg cgatcccggt cctcgacgag gaggcgcacc
       61 tcggcgcctg cctcgacgcg atcgaggcgc agacctactc cggcatcgtc gaggtgctcg
      121 tcatcgacgg cggttcgagc gaccggagcg tcgagattgc tcgacagcac cagatggtcc
      181 gggtcctcga caacccgcgc cgcatccagt cggccgcgtt gaaccgggcg ctcgacgagg
      241 cggccggcga gatcctggtt cgggtcgacg ggcactgcgt catcgccccc gactacgtcg
      301 agcgctgtgt cgctgcgctc gatgcgaccg gtgccgcgat cgtcggcggt ggtatgaccc
      361 cagtcgccaa cggtgcgatg caggaggcga tcgccgccgc catgggctcg aggatcgggg
      421 ccggcccggc gcggttccac agctctggcg acgccggatg ggtcgacacc gtgtacctgg
      481 gcgcctaccg ggtggccgac gcggttgcgg tgggcggata cgcggccgat atgagcatca
      541 acgaagacgc cgagttcgcc attcgtctgg catctcgtgg cggggtctgg ttcgacccga
      601 cgatccgctc gacctacaca ccacgttcgt ccttggctgc cgtcgcccgg cagttctacc
      661 gctacgggcg aggacgtgcc gtgacggttc ggcggcaccc ggcgtcgctg tcgccgcgcc
      721 agctcgcggc gcccgccttg gtgttgggaa tcctcagccc gtggcgtcgc ccggtactcg
      781 gcgcgtacgc cgcggtcgtc gccgggtggt cgttcgccgg gcgttcggat cgcccggccc
      841 gggcgggtct ggtccttccg gcgatgcacg tcccgtgggg tgtcggcttc ctcaccggtc
      901 tggttctggg cggccgcagc tagcctgacg ggcatctccg tcgccgttgt catccccgca
      961 ttcgaagcag ctccggtgct cgacacgacg cttgcgtcgg tggtcggcca gacgctcgcg
     1021 cccacggaga tcgtggtggt cgacgacggg tcgaccgacg acacggcggc cgtcgccgag
     1081 cgctggagcg aggtcgctcc cgtgcgggtg gtgcgccagc gcaaccaggg gcccgcagcg
     1141 gcgcgacgcg ccggcgtcgc ggccaccacg gcgccgctcc tggcattcgt cgacgccgat
     1201 gacgtgtggt tggcggacca cctcgacgtc gctgtcgcag tacacgcccg gtccggcggg
     1261 ctcgtctcgc ccgacgcgtt ctcgtggcgg cccggctcgg ccctgccgtc ggcgacacgg
     1321 cgacgacggt ttccgatccc tgcaccgcac cgacaacgga ccggcatcgt gcgcgagaac
     1381 ttcgtcttca gctccgtcgt cgtgagccgc cgcgactacg aggccgccgg cgggttccgc
     1441 gacggcgtga ccggcgccga ggactgggac ctgtggatcc ggatggtccg ccggggcacg
     1501 ctcgttcgcg ggagcgaccg accgacgtgg ctgtaccgcg tcggcggggt cagcctcacc
     1561 gaccgacccg acatgcgagc gacgtacgag gaggtactcc gccgggtcct cgccgacagc
     1621 gacgatgcgg gggagcggcg ggcagccgag gaacacctcg gccgattgca ggcccggggg
     1681 caactcgacc gcgcctacgc cgcggcgcgc tccggcgacc ccggcgtggc ccgtcgactg
     1741 gcgcgctccg cgctgcacgg ccggcctcgc gtcgcagcgg aggcgctgtc ggtggcagtc
     1801 gctccgggcc tcgccacccg gctcggcgat gaggcccggg cacggtggac ggccggtcgc
     1861 cgatgagccg gacgcacttc cacaccgact gctactcgtt cagcgggagt gagacgacct
     1921 tgatgctcct gctcgacgcg gccgctcgtc gtggcgacga gttcacgttc acctatcgcc
     1981 gctggcccga gtacgagcac ggcctgttcc ggcgtcttcc ggcaggggtg cgggcgcgtg
     2041 gtctccgcct ccccgatccg gcggacgtga agcacgccgt caccggcggt cgcccgggga
     2101 cgggagctcg gctggtccgc ggcgcaatca cgttgctccc gttccgccag ctgttcctgg
     2161 cgtacgacac ggtgagactc actgcactgc tgcgcaagga gcggcccgat gtcctgcacg
     2221 tcaacaacgg cgggctaccc gggtcgatct cctgcaacgc tgcggcgatc gcgggtcggc
     2281 tcgccggtgt gccggtggtg gtcatggtgg tcaacaatct cgccttcgcc tacaaccggc
     2341 ccggccggtg gttcgattac ccggtcgacc ggctgctcgt gcggtccgtc acccggttcg
     2401 tcaccggctc cgcagcggcg ggcaccgcgc tccgtcgggt actgcgactt cccgaagggc
     2461 agcacgtcgt gatcccgaac ggagtcgagc aacggctccc gacagcgtcc gcggacgaga
     2521 cgcggagcgc gctcgggctg tcgccgggga ttgccgtggt ctcggtcgtc gctcggctgg
     2581 aggctcggaa aggacaccgg cacctcctcg acgccctcag gctgctcccc gcggatcggc
     2641 gtgctgcgct ccgggtcctg ttcgccgggg ccggtcccga acgcggtcgc ctcgaacagg
     2701 aggtgacccg cctcggactg accgacgtcg tgcagttcct cggcgaccat cccgatccgt
     2761 ggtcgttgta cgaggtgtcg gacctggtgg tcctgccgtc ggtccgccac gaggacctgc
     2821 cgatcgtgct catcgaggcg atggcggcgg gcgttcctgc agtggccagc cgcgtggccg
     2881 gcaccgtcga gctgatcgag gcgggcgtca ccggcctgct cgtcgagccg gccgaacccc
     2941 gacagctcgc cgacgcgatc gccggactcc tcgacgaccc cgatcggcga cgggcgatgg
     3001 gcaaggccgc cacacttcga tacgaggcga cctacacccc ggcgcaggcg gtcgccgcct
     3061 atcgcgatct caccgaccgc cttctcacgg gggtgacggc atgatcctcg atctcggatg
     3121 tggatcgtcc aagcgacccg gcgccgtcgg cgtcgacatc ggcccgttcc ccggtgtcga
     3181 tgtcgtgcac gatctgaatc ggcacccgtt ccccttcgag gacggcgtgg ccgacgaaat
     3241 ctacctcgac aacgtgttgg agcacctcga cgatgtgatc gccacgatgg aggaactcca
     3301 ccgcatcggc cgcgacgggt gccggatccg catcgacgtt ccgtacttcc gctcccgctg
     3361 ggcggcgatg gatcccaccc acgtgcacgc gttcacgccg gagtccttcg cgtacttcga
     3421 tccgacccac cccttcagcg aacggtacgg ctattcagcg gttcgattcg acgtcgaatc
     3481 cgtcgtctac aacgagcgct tcccggcccg gggcccgcgg gcactcgtcg ctcggttcgc
     3541 caaccgccga gccgagcgct acgagagcta cctcgccccg atcatcccgc tcgacgagct
     3601 cacgttcacg ctgcgcgtcc gcaagcacgc gcgatgacgg cctcccggct ccgcctcggc
     3661 gtgtacgccg acctccgcta ccagcgcggg gcggagggcg tgacgtgcga caccgccttc
     3721 gttcggttca tcgccgcgct cggccggcac gtcgaccacc tggtcgtgtt cggccggctc
     3781 ggcgaggtgg tgtcgccgat ggccacgctg ggcaccgacg aggccgagtt cgtgccgttg
     3841 ccggactacc ggtccctcgc cgacgtcggt gccgtatccc aggcgatcgc ccccagccgc
     3901 agagcctggc ggcgacccct ggaggcgctc gacgcggtcc tcgtcttcgg tccgcacccc
     3961 gtggcgaccg cactcgtgcg tgacgctcgg aggcggggtg tcgcagtggt gttgggcgtt
     4021 cgccaggacc tgcccggcta tctggggctc cgggcctcgg gccgcacgcg tccggtggtc
     4081 ctcgctgccg cctccggcct cgagtggggc tggcggcgat ccgctcgacg ccagccggtg
     4141 gtggtgaccg gctcggatct cgcccgcaac taccgcaacg cgacctggct cctggacgtg
     4201 tcct
//
LOCUS       DAOSOA010000297         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21286_length_4190_cov_7.679323, whole genome shotgun sequence.
ACCESSION   DAOSOA010000297 DAOSOA010000000
VERSION     DAOSOA010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21286_length_4190_cov_7.679323"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..959)
                     /locus_tag="PKY13_14405"
     CDS             complement(57..959)
                     /locus_tag="PKY13_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT81183.1"
                     /translation="MTEQIYRGIPRPPAPGATALHPGAFDGETVFVTGGGTGLGKAIA
                     SEFARLGANLVIASRKPEHLEAGNNAISSLGGEVLTVACDIREPDSIAAAFDAAEERF
                     GRPGVLINNAAANFPVPAEDMSPNAWRTVVDITLNGTFFCCREFGRRHIEAGTSASIV
                     NIGASYAWTGGPGFAHSSAAKAGVKNLSETLAVEWGPFGIQVNCLVPGLFPHEDMTAD
                     IKGNLAGTSDKDVCQPALRTGERQELGWAATFLASPYGRFISGHTLVVDGANWQRRAL
                     VNPPVTPIREQMGRGPFEPNPPKP"
     gene            complement(964..1743)
                     /locus_tag="PKY13_14410"
     CDS             complement(964..1743)
                     /locus_tag="PKY13_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT81184.1"
                     /translation="MTGSVHLEIDGAIATLINDNPTKHNAFDDEMDAQLFAALAELKA
                     RKDVRAVIWRGVGPSWSSGRDVGSIGTNKTELTHHELMTRGHAGIQQIWDIEAPIIVA
                     IQGWALGGSFQRALLCDIRICSTDAKFMLPEVGHGVIPDTGGVAVLQQMCGPGLVSDM
                     VLTGRRLVAEEALYHGIVSRVVDPDVLVDTANEMAARIASSPAVAVKLARRVISHLGR
                     PAVRTSMDDELIYQTFLNRSDDFAEFRAAKAEGREPNYRGS"
     assembly_gap    1857..1956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1957..3075)
                     /locus_tag="PKY13_14415"
     CDS             complement(<1957..3075)
                     /locus_tag="PKY13_14415"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT81185.1"
                     /translation="METLVVPQGEFELARFPVRKNDRLRAWDAADEYLLQHVADSRPH
                     GDQPAEPAVLVVNGGPGALAVGLVAGGATDVRVMSDSYLDHVGTEENLRRNGFDPAAV
                     TLMGPFGLDAPEAPPVDLVLMKIPKSSALLEDQLCRISPHLSPGAVVVAAAMSKHIHT
                     STLQLFERILGPTTTSLARKKARLVECSPAADRQPATSPWPARIPTGGGGLVLVNHAN
                     VFSREKLDIGTRFLLDHLPAVEGPAHVVDLGCGNGVVGLAIAATAPEAHVTFVDESFM
                     AVASAAATYAENLGEDRAATSARFAVGDTLFHLQSGDPIERGSIDLVVNNPPFHDDHA
                     VGDAIAWQMFTEAHGALAIGGELRVVGNRHLAYHAKLKR"
     gene            complement(3122..>4190)
                     /locus_tag="PKY13_14420"
     CDS             complement(3122..>4190)
                     /locus_tag="PKY13_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HOT81186.1"
                     /translation="MVEIEDPPPPPKGKSGSKSALAAEVRELRDYLDRLGFPERRALR
                     ADIDSLVAHRTEVLAEVRAATLARREQEVALVRVRREAVLQDVGIYRPRHPAEDSVAL
                     RSRLETTQAAIEELVKAGDAVSATDDWTVNGSKAEGLKIVGELAQLLLRTYNAEADLL
                     VATLRPCELDAAVDRLAVTLATVHRLGASMEIEISDELHRLRVEELSLAADELALRDE
                     ARRRALDADRLPDGAAGNLYVVSNRGAFGPDVVRIGVTELDQPAEVVDHVDAGRLPFR
                     SDLHALVPSPDAAELLAGVRSALDDRRVNLTDPQCPFFSVSPDVVRDVLVDLAVTVPW
                     FVATGTAEEWHQSRNARDEND"
BASE COUNT          618 a         1422 c         1438 g          612 t
ORIGIN      
        1 cggggttttg tagtgcgagg agcgacgctg gcgtcgctcc tcgcagcaca aaacccctac
       61 ggcttgggcg ggttcggttc gaagggaccg cgacccatct gctcccggat gggggtgacc
      121 ggcgggttca cgagggcgcg acgctgccag ttggcaccgt cgacgaccag ggtgtgcccg
      181 ctgatgaacc gcccgtaggg cgaggcgagg aacgtcgccg cccaccccaa ctcctgacgc
      241 tcgccggtcc ggagcgccgg ctggcagacg tccttgtcgg aggtgccggc gaggttgccc
      301 ttgatgtcgg ccgtcatgtc ctcgtggggg aacaggccgg gcaccaggca gttgacctgg
      361 atgccgaacg gaccccattc gacggcgagg gtctccgaga gattcttcac cccggccttc
      421 gccgccgacg aatgggcgaa cccggggccg cctgtccacg cgtaggacgc tccgatgttc
      481 acgatcgacg ccgaggtgcc cgcctcgatg tgacgtcgcc cgaactcgcg gcagcagaag
      541 aacgtgccgt tgagggtgat gtcgacgacg gtccgccacg cgttgggcga catgtcctcg
      601 gccggcaccg ggaagttggc ggccgcgttg ttgatcaaca cgccgggacg gccgaagcgt
      661 tcctcggcgg cgtcgaacgc tgcagcgatg gaatcgggtt cgcggatgtc acacgcaacg
      721 gtcagcacct cgccgccgag cgacgagatg gcgttgttgc cggcctcgag gtgctcgggc
      781 ttgcgggacg cgatgacgag gttcgcgccg agccgggcga actccgacgc gatcgccttg
      841 cccagaccgg tgccgccacc ggtgacgaac acggtctcgc cgtcgaacgc gcccgggtgc
      901 agtgccgtag ccccgggagc gggcgggcgg ggaatgcccc ggtagatctg ctcggtcatc
      961 ggctcagctc ccccggtagt tcggctcgcg tccctcggcc ttggctgccc ggaactcggc
     1021 gaagtcgtcg ctccggttga ggaacgtctg gtagatcagc tcgtcgtcca tcgacgtccg
     1081 cacggcggga cgcccgaggt ggctgatgac ccggcgggcc agcttcacgg ccacggcggg
     1141 cgacgacgcg atccgcgccg ccatctcgtt ggccgtgtcc accaggacgt cgggatcgac
     1201 gacccgcgac acgatgccgt ggtacagcgc ctcctcggcc acgaggcgcc gaccggtgag
     1261 caccatgtcg ctcacgaggc cgggcccgca catctgctgc agcaccgcga ccccaccggt
     1321 gtcggggatc accccgtggc cgacctcggg cagcatgaac ttcgcatccg tcgaacagat
     1381 gcggatgtcg cacaggaggg cccgctggaa tgaaccaccc aacgcccacc cctggatggc
     1441 gacgatgatc ggcgcctcga tgtcccagat ctgctggatg ccggcgtgac cgcgagtcat
     1501 caactcgtgg tgggtcagct cggtcttgtt ggtgccgatc gatccgacgt cacggcccga
     1561 cgaccatgac gggccgacgc cgcgccagat caccgctcga acgtccttgc gcgccttcag
     1621 ttcggcgagg gccgcgaaca gctgagcgtc catctcgtcg tcgaacgcgt tgtgcttggt
     1681 cggattgtcg ttgatcagcg tggcgatggc gccgtcgatc tcgaggtgta cggatccggt
     1741 cacggagtcc ccctgtggcg tgccgcgccc gacgaagggc gggagctgaa ctgtaccgaa
     1801 cccgctccga cccccgaccg ctcacctttc gcgggaagtt gtcgtcaccc cctgggnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgc ttgagcttcg cgtggtaggc
     1981 gaggtggcgg ttgccgacga ctcgaagctc gccaccgatc gcgagggcac cgtgggcctc
     2041 ggtgaacatc tgccaggcaa tcgcatcgcc gacggcgtgg tcgtcgtgga acggcgggtt
     2101 gttgaccacg aggtcgatgc tgccccgctc gatcgggtcg ccgctctgca gatggaacag
     2161 ggtgtcgccg acggcgaacc gggcagaggt ggcggcacga tcctcgccga ggttctcggc
     2221 gtaggtcgcg gccgccgacg ccaccgccat gaacgactcg tcgacgaacg tcacgtgggc
     2281 ctcgggtgcg gttgcggcga tcgccaggcc gacgacgccg ttgccacacc cgagatcgac
     2341 gacgtgggcc gggccctcga ccgccgggag gtggtcgagg aggaaccggg tgccgatgtc
     2401 gagcttctcg cgggagaaga cgttggcgtg gttgacgagc acgagcccgc ccccgccagt
     2461 cgggatgcgc gccggccacg ggctcgtcgc cggttgccgg tcggccgcgg gcgagcactc
     2521 gacgaggcgc gccttcttgc gggcgagcga cgtggtcgtc ggaccgagga tccgctcgaa
     2581 cagctgcagc gtggacgtgt ggatgtgttt cgacatcgcg gcggccacca ccaccgcccc
     2641 cggagacagg tgcggggaaa tccggcacag ctggtcctcg aggagcgcgg agctcttcgg
     2701 gatcttcatg agcacgaggt cgacgggcgg ggcctcgggc gcatcgagtc cgaacgggcc
     2761 catcaaggtc accgccgccg gatcgaaccc gtttcggcgc agattctcct cggtgccgac
     2821 gtggtcgagg taggagtccg acatcacccg cacatcggtg gcaccgccgg cgacgagacc
     2881 gaccgccagc gcgcccgggc cgccgttcac cacgagcact gcgggctctg cgggctgatc
     2941 gccgtgggga cgggaatcag cgacgtgttg gagcaggtac tcgtcggccg cgtcccaggc
     3001 gcgcagccgg tcgttcttcc gcaccgggaa tcgggcgagc tcgaactcgc cctggggaac
     3061 gacgagggtc tccatcccgc acgacgctac cggacccggc cgcccgaccg agctggtggg
     3121 ttcagtcgtt ctcgtcgcgg gcgttgcgcg actggtgcca ctcctccgcc gttcccgtcg
     3181 ccacgaacca cggaacggtc acggcgaggt cgacgagcac gtcgcgcacc acatcgggcg
     3241 acaccgagaa gaacgggcac tgcggatcgg tcaggttcac gcgccggtcg tcgagcgccg
     3301 accgcacgcc ggccagcaac tcggcggcgt cgggactggg cacgagcgcg tgcaggtcgg
     3361 agcggaacgg gagccggccg gcgtcgacgt ggtcgaccac ctcggccggt tggtcgagct
     3421 cggtcacccc gatgcgcacc acgtcgggcc cgaacgcgcc ccggttggac accacgtaca
     3481 gattcccggc ggcaccgtcg ggcaagcgat cggcgtcgag cgcccggcgc cgggcctcgt
     3541 cacggagcgc cagctcatcg gctgccaacg acagctcctc gacccgcaat cggtgcagct
     3601 cgtcggagat ctcgatctcc atcgacgccc cgagccgatg caccgtcgcc agggtcacgg
     3661 cgaggcggtc gaccgccgcg tcgagctcgc agggccgcag cgtcgcgacc agcagatcgg
     3721 cctcggcgtt gtaggtgcgc aacaacagct gcgcgagctc tcccacgatc ttgagccctt
     3781 cggccttcga cccgttgacc gtccagtcgt cggtggccga caccgcgtcg cccgccttca
     3841 ccaactcctc gatcgctgcc tgtgtcgtct cgagacgcga gcggagcgcc acgctgtcct
     3901 ccgccggatg gcgcggccgg tagatcccga cgtcctggag cacggcctca cgccggaccc
     3961 ggaccagtgc gacctcctgc tcacggcggg caagcgtcgc cgcccggacc tcggcgagca
     4021 cttcggtgcg gtgggcgacg agggagtcga tgtcggctcg cagcgcccgc cgctccggga
     4081 acccgagtcg gtcgaggtag tcacgcagct cgcgcacctc cgccgccagc gccgacttcg
     4141 agcccgactt gcccttcggc ggaggaggcg gatcctcgat ctcgaccatc
//
LOCUS       DAOSOA010000298         4175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21379_length_4175_cov_5.583981, whole genome shotgun sequence.
ACCESSION   DAOSOA010000298 DAOSOA010000000
VERSION     DAOSOA010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21379_length_4175_cov_5.583981"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..525
                     /locus_tag="PKY13_14425"
     CDS             169..525
                     /locus_tag="PKY13_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT81187.1"
                     /translation="MVVEIPTETPPQRSDDDVADELFHALADATRRDIVRRTMVSEHS
                     VSALARMYPMSFAAVQKHVVVLTRAGLVTKEARGREQIVRCDIATVHTAQRLLDQLEQ
                     VWRDRLGRFGSVLAES"
     gene            583..1119
                     /locus_tag="PKY13_14430"
     CDS             583..1119
                     /locus_tag="PKY13_14430"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOT81188.1"
                     /translation="MTVTDVQQDPTTLTLTIRSEYTSPIDRVWQMWADPRKLERWWGP
                     PTFPATVTEHNLTTGGRVVYFMTSPEGDRHFGWWEFKAVDAPTSLRFEDGFGDETGAP
                     IPDMPVSSIAVDLADSDSDSGSGSGSGSGSDSGTTMTIATTFPSAEAMDQMISMGMAE
                     GMALALGQIDDLLAADNA"
     gene            complement(1142..1687)
                     /locus_tag="PKY13_14435"
     CDS             complement(1142..1687)
                     /locus_tag="PKY13_14435"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOT81189.1"
                     /translation="MPEPTMPEIAEPETAGSDRSVSPPDIPLHTLLDLQVLPEVEGST
                     VSEVLMPVAPGAFGFTRNLHGGAIATLVDLACAIAAVRATGFDPSRESLVTSDMHLRY
                     VGRPGTGNVIARSEVVRAGSQLIVIDCRVVDEDDHLVAVADLSMMRVSVRRPLDGPDA
                     GAAPDAVGASSGSETAGRTVD"
     gene            complement(1713..2132)
                     /locus_tag="PKY13_14440"
     CDS             complement(1713..2132)
                     /locus_tag="PKY13_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT81190.1"
                     /translation="MGDRTDVAAIERLKARYFRTLDTKDWDGLVRVFTDEVTVDVAGS
                     GGPTTTSAVAFVERVRRSLGDAITVHHGHMPEIDITSETTATGIWAMEDKIWWPDGSA
                     VRFMHGYGHYHETYVRLASGWRIATMQLTRLHREFEP"
     gene            2189..3394
                     /locus_tag="PKY13_14445"
     CDS             2189..3394
                     /locus_tag="PKY13_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT81191.1"
                     /translation="MTDPTRTGPADGAPTIDVVGLIDVDRVVADLVTMVGIPSVNPFA
                     GPASPTAREHELAAWYAAALTELGFDVHVDEVVPGRPNVVGRLRGTGGGASVLLAGHL
                     DTVGVHDYDTAFDATVADGRVHGRGSCDMKAALAAYLEVARVLARSGTRLAGDLVIAG
                     LCDEEHTMIGSRRLGDGTPLADAAIIGEPSNLDVCPAHKGQVCVIVRTHGRAVHSSRP
                     ELGRNAIVDMATVIGRLDAYAAELSAREPHPLCGHARVTAAVISGGTIASTVPDRCEL
                     EIDRRTLPGETPEQVLTELAALLDPLVAADPGFRYELVGPTLVCDPLDTPVDAPVVVA
                     LLDAVGGRSATAFPAATDAPNLGIPAVVCGPGRLEEAHTTNEYVEIAQLGAAVGIYLS
                     SVLRLTAPA"
     gene            3403..>4175
                     /locus_tag="PKY13_14450"
     CDS             3403..>4175
                     /locus_tag="PKY13_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOT81192.1"
                     /translation="MTTPRELADRIEARLAPLATEAGRAWWDASVDASPHHDERRVAA
                     EIAYADALADPDTFATLRNVLAAVDPTGEPELHRRLVLLHNEFVGNQVDPALMERLIQ
                     LGAEVDSAFSAFRGTVGDRNLNDNEINAILRDSDDSGERRQAWEASKQVGAEVADRVL
                     ELVRLRNEIAVGLGYRDHFAMSLALTELDEDVVFSVLDDVDRVSAEPFARWKSGVDTR
                     LAERFGCGVGDLRPWHYDNVFFQSPPAGSGADLDAWYRGR"
BASE COUNT          623 a         1539 c         1395 g          618 t
ORIGIN      
        1 cggaggccgg tctcggggct caagtggtac aagcaggtca agcgcgactt cgccgagctc
       61 gctgcggact gagcggggtc ggtgtgccag gggccggacc ctgttgtcga cggcctcgga
      121 gcggacacgc ttgtgcgccc ggggagtcgc tcgataacct acaaccgaat ggttgtagaa
      181 atccccaccg agacgccacc gcagcgatcc gacgatgacg tcgccgacga gctgttccac
      241 gcgttggccg acgccactcg gcgcgacatc gtccgtcgca cgatggtgtc ggagcactcg
      301 gtgtcggcgc tggcccggat gtacccgatg agcttcgccg ccgtgcagaa gcacgtcgtc
      361 gtgctcacac gggcgggtct cgtcaccaag gaggcccgag ggcgcgagca gatcgttcgc
      421 tgcgacatcg cgaccgttca cacggcccaa cgcctactcg accagctcga acaggtgtgg
      481 cgcgaccgtc tcggtcggtt cggatcggta ctcgccgagt cctgacgccc atccgaggcg
      541 cggggccccg gaacccaatc agatcaccac caaggagccc acatgaccgt gaccgacgtc
      601 cagcaggacc cgaccaccct gaccctgacc attcggtcgg agtacacctc gcccatcgac
      661 cgggtgtggc agatgtgggc cgatccgcgg aagctcgagc gctggtgggg gccgcccacg
      721 ttccccgcca ccgtgaccga gcacaacctc accactggcg gccgggtcgt ctacttcatg
      781 acgagccccg agggtgaccg ccacttcggc tggtgggagt tcaaggccgt cgacgctccc
      841 acgtcgctcc ggttcgagga cgggttcggc gacgagaccg gtgcgcctat ccccgacatg
      901 cccgtctcgt ccatcgccgt cgacttggcc gactccgact ccgactccgg ctccggctcc
      961 ggctccggct ccggctccga ctccggcacg accatgacca tcgcaacaac gttcccgtcc
     1021 gccgaggcca tggatcagat gatctccatg ggcatggccg agggcatggc gctcgccttg
     1081 ggccagatcg acgacctgct cgcagcggac aacgcctgac cgagcgctcg acccggcgcg
     1141 ctcagtcgac cgtgcggccg gcggtctccg accccgagga ggccccgacc gcgtcgggcg
     1201 cggcgccagc gtcgggcccg tcgagcggcc ggcgcaccga cacccgcatc atcgacaggt
     1261 ccgcgacggc cacgagatgg tcgtcctcgt cgacgacccg gcaatcgatg acgatcaact
     1321 ggctcccggc ccgcaccacc tcggaacggg cgatcacgtt gcccgtcccc ggccgcccca
     1381 catagcgcag gtgcatgtcg gacgtgacga gcgactccct ggacggatcg aaccccgtcg
     1441 cccgaaccgc cgcgatcgca cacgccaggt cgacgagcgt ggcgatcgca ccgccgtgca
     1501 ggttgcgggt gaacccgaag gcccccggcg ccaccggcat gagcacctcc gagaccgtcg
     1561 acccctcgac ctctggcagc acctgcaggt cgagcaacgt gtgcagcggg atgtcgggcg
     1621 gggacacgga acggtcggat ccagcggtct cgggctcggc aatctcgggc atggtgggct
     1681 cgggcatggt cggtcagcct aggacgcggt ggtcagggct cgaactcccg gtgcagccga
     1741 gtgagctgca tcgtcgcgat gcgccacccg ctggcgagcc gcacgtaggt ctcgtggtag
     1801 tggccgtacc cgtgcatgaa gcggactgcc gaaccgtccg gccaccagat cttgtcctcc
     1861 atcgcccaga taccggtggc ggtcgtctcc gacgtgatgt cgatctccgg catgtgaccg
     1921 tggtgcacgg tgatcgcgtc gccgagcgat cgccgcaccc gctccacgaa cgcgacggcg
     1981 ctcgttgtcg tcggaccgcc cgagccggcc acgtcgacgg tcacctcgtc ggtgaacacc
     2041 cgcaccaacc cgtcccagtc cttggtgtcg agcgtacgga agtaccgggc cttgagtcgt
     2101 tcgatggctg ccacgtcggt ccggtcgccc atggcaggat cgtacgtgtc ggcgcccgtg
     2161 cgcgaccgtc ccctccgagg agcacccgat gaccgacccc acccgcaccg gaccggccga
     2221 cggggcgccc acgatcgacg tggtcgggct gatcgacgtc gaccgggtcg tcgccgacct
     2281 cgtcacgatg gtcggcatcc cgagcgtcaa cccgttcgcc ggcccggcat cgcccacggc
     2341 gcgcgaacac gagctcgccg cctggtatgc cgcagcgctc accgaactcg gcttcgacgt
     2401 gcacgtcgac gaggtcgtgc ccggtcgccc caatgtggtc ggccggctcc gcggcaccgg
     2461 cggcggcgcg tcggtcctgc tcgccgggca cctcgacacc gtcggggtcc acgactacga
     2521 cacggcgttc gacgcgaccg tggccgacgg ccgggtgcac ggtcgcggat cgtgcgacat
     2581 gaaggcggcg ctcgccgcgt acctcgaggt ggcgcgggtc ctggctcggt cgggtacccg
     2641 cctcgccggc gacctcgtga tcgccgggct gtgcgacgag gagcacacga tgatcggctc
     2701 gcgccgtctc ggcgatggca cgccactcgc cgacgccgcg atcatcggcg agccgagcaa
     2761 cctcgacgtg tgtccggcgc acaaggggca ggtctgcgtc atcgtgcgca cccacggccg
     2821 ggcggtgcac tcgtcgcgcc ccgaactcgg ccgcaacgcc atcgtcgaca tggcgaccgt
     2881 gatcggtcga ctcgatgcct acgccgccga gctgtccgca cgcgagccgc acccgctgtg
     2941 cgggcatgcc cgggtcaccg cagcggtcat cagcggcggc accatcgcgt cgaccgtgcc
     3001 cgaccgctgt gagctcgaga tcgaccggcg caccttgccc ggcgagacgc ccgagcaggt
     3061 gctgaccgag ctggcggcgc tcctcgaccc gctggtcgcc gctgatcccg ggttccgata
     3121 cgagctggtg gggccgacgc tcgtgtgcga cccactcgat acgccggtcg acgccccggt
     3181 ggtcgtcgcc ctgctcgacg cggtcggcgg ccgctcggcg acggcgttcc ccgccgccac
     3241 cgacgccccc aacctcggca tcccggccgt cgtgtgcggc ccgggccgcc tcgaggaggc
     3301 gcacacgacg aacgagtacg tcgagatcgc ccagttggga gcggccgtgg gcatctacct
     3361 gtcatcggtg ctccggctca ctgccccggc ttgaatgtgg acatgaccac gccccgcgag
     3421 ctcgccgacc gcatcgaggc gcggttggcg cccctggcga ccgaggccgg cagagcgtgg
     3481 tgggacgcca gcgtcgacgc ctcgccccac cacgacgagc gtcgtgtcgc cgccgagatc
     3541 gcctacgccg atgcgctcgc cgatcccgac acgttcgcga cgctccgtaa cgtgcttgca
     3601 gcggtcgacc cgaccggcga gcccgaactc catcgtcgcc tcgtgctcct ccacaacgag
     3661 ttcgtcggca accaggtcga tcccgccctc atggagcggc tcatccaact cggcgccgag
     3721 gtcgactcgg cgttcagcgc ctttcgtggc acggtcggcg accggaatct caacgacaac
     3781 gagatcaacg cgatcctgcg cgactccgac gactcaggcg agcgccgcca ggcgtgggag
     3841 gcgtccaagc aggtcggtgc cgaggtcgcc gaccgggtgc tcgagctcgt gcgcctccga
     3901 aacgagatcg ccgtcggtct cggctaccgc gaccacttcg ccatgtcgtt ggccctcacc
     3961 gaactcgacg aggacgttgt gttctcggtg ttggacgacg tggaccgcgt ctcggccgag
     4021 ccgttcgccc ggtggaagtc cggcgtggac acgcgcttgg ccgagcggtt cggctgtggt
     4081 gtcggcgatc tgcggccgtg gcactacgac aacgtgttct tccagtcgcc gcccgccggt
     4141 tccggtgcgg acctcgacgc ctggtatcga ggccg
//
LOCUS       DAOSOA010000299         4154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21519_length_4154_cov_5.511832, whole genome shotgun sequence.
ACCESSION   DAOSOA010000299 DAOSOA010000000
VERSION     DAOSOA010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21519_length_4154_cov_5.511832"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKY13_14455"
     CDS             <1..477
                     /locus_tag="PKY13_14455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT81193.1"
                     /translation="ASATFVGTSLTLYGVPLVLLSGRGGRLVDRLGPMTAGKRAITCA
                     IPVIVAYGVLDSYWIVALTAIVEAVIQAVASPSAQAAMAKACPPDRIGAGQGLAGAFG
                     LSGGGLLASVAPAVYDRYGAGVLFTSVGVLVGAIAIAAFALDASANARLTPATQPA"
     gene            complement(452..1060)
                     /locus_tag="PKY13_14460"
     CDS             complement(452..1060)
                     /locus_tag="PKY13_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81194.1"
                     /translation="MQVYQKRPSGLGAKELSAHARRDRGGALFAVGRAVVGVVLIGLI
                     GAPLGFLWFVLGVASLASVFLFAKAAGDAALVRPALVLTGRPKWDGTRRTAWSVVSDD
                     LPDTLQRLASGLDAALGASLTAYDEGSTYRGITRREAFDAPEHPVVFAWGIDGKARLK
                     ATDDVSGVWIRMEYNTADALVATVLANEGRAVDALTQVAWRA"
     gene            complement(1098..2006)
                     /locus_tag="PKY13_14465"
     CDS             complement(1098..2006)
                     /locus_tag="PKY13_14465"
                     /inference="COORDINATES: protein motif:HMM:NF005861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT81195.1"
                     /translation="MSGICDGRVVIVTGAGRGLGRAHALAFAAEGAKVVVNDIGAESD
                     GSGGSLSPAQEVVDEITAAGGEGIVNGDDVSSVEGAERLVNMAIETFGGLDVVLNNAG
                     IVRDRMFANATADEWDAVMQVHLKGHFLPAKFAAGYWRDQSKAGKPVSGRIINTSSGA
                     GLMGSIAQAAYSAAKGGIASLTLVQAAELGRYGVTANALAPSARTRMTEEAFAEMMAA
                     PTDGSFDSMAPENVSPAVVWLGSEQSSHVTGRVFELEGGKIGIAQGWMHGPTFDKGAR
                     LEPAEVGAIVADLLAEAAKPTPVYGT"
     gene            2131..2970
                     /locus_tag="PKY13_14470"
     CDS             2131..2970
                     /locus_tag="PKY13_14470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOT81196.1"
                     /translation="MRYGVTIFATDQAMPADEVAVEAEARGFDSLYLPEHTHIPVSRR
                     TPPPTGDAELAEEYKRTVDPLIALAAAAARTTTLRLGTGVVLPAQREPIVTAKAIATL
                     QNLSGGRFCLGVGFGWNEDEMADHGVDYRTRRARSREHVLAMQSLWTEEVGGFDGEYV
                     KIAPSWSWPKPDVEVPVLVGGGAGPKLFSHIAEYGHGWIPIGGAGLTDDIGRFKETVS
                     AAGRDPEAMEIVPFGSLPSPSKLDHFESIGVTECVFRIPSAPRDVVLPVLDEQAALLA
                     TRR"
     gene            complement(2967..>4154)
                     /locus_tag="PKY13_14475"
     CDS             complement(2967..>4154)
                     /locus_tag="PKY13_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81197.1"
                     /translation="RWKRLISKADGLRKVAEIKAAEERAKLPPPTTAPVVDVPVDADP
                     PAYQEPIDDLPAEEPSSNATVSTVLAAVADPPCSGCSLEFVVSASGRFVAFETNRAFD
                     PADTDFAPDLYLLDTTTGSVRWISRSTAFGTQYWAYNSLGMDDAGTTVVYVTPVGDVT
                     ETFTYDIASDTLTQPVGRGAQAVAISANGRHLAIAGSDADRIFMIDRTTGERTTRRFQ
                     RSITTLTSLNVSDDGQVVTGGAHFAAADGVGSGDDQLFRDTEASGTMLYPARTSNLLT
                     EATADGRFLVTAGNGTGWLLDTMTGATADLTDGCSWDGLCVGGSVDISSDGRFVSFLG
                     YPAYSAARAYVIDLDDGGYWYAGNGAVAVGLTDDGMMFLAPPDGGSTTLGLSLLGPLR
                     SAS"
BASE COUNT          627 a         1453 c         1444 g          630 t
ORIGIN      
        1 gccagcgcca ccttcgtcgg cacgtccctc acgctctacg gggtccccct cgtgttgctc
       61 tcgggacgag gcggtcgcct ggtcgaccgg ctcgggccga tgacggccgg caagcgcgcg
      121 atcacctgcg ccattcccgt catcgttgcg tacggcgtgc tcgactcgta ctggatcgtg
      181 gcgctgacgg cgatcgtcga ggccgtgatc caggcggtgg cgtcgccctc cgcgcaggcg
      241 gccatggcca aggcgtgccc gcccgatcgc atcggtgccg gtcaggggct tgccggcgcc
      301 ttcggcctca gtggcggcgg cctgctcgcc agcgtcgctc cagcggtcta cgaccgatac
      361 ggcgccggtg tgctgttcac gagcgtgggc gtcctggtcg gcgcgatcgc gatcgcagcg
      421 ttcgcgctcg atgcatcggc gaacgctcga ctcacgcccg ccacgcaacc tgcgtgagcg
      481 cgtcgacggc acgaccttcg ttggcgagca cggtcgcgac gagggcgtcg gcggtgttgt
      541 actccatgcg gatccacaca ccactgacat cgtcggtggc cttgaggcgc gccttgccgt
      601 cgatgcccca ggcgaacacg acagggtgct ccggcgcgtc gaacgcctcg cgccgcgtga
      661 tgccccggta ggtcgacccc tcgtcgtacg ccgtgagcga cgccccgagc gcggcgtcga
      721 gcccgctcgc caatcgctgc agcgtgtcgg gcaggtcgtc ggagacgacc gaccatgcgg
      781 ttcggcgagt gccgtcccac ttgggtcggc cggtgagcac gagcgccggg cggacgagcg
      841 ccgcgtcacc cgccgccttc gcgaacagga agaccgaggc cagcgaggcg acgcccagca
      901 cgaaccagag gaacccgagc ggcgcaccga tcagcccgat gagcaccacg ccgacgacgg
      961 cgcggccgac ggcgaacagg gcacctccgc gatcgcggcg cgcgtgcgcc gacaactcct
     1021 tcgccccgag gcccgagggc cgcttctggt agacctgcac ggccgtagtc tcgcagcggc
     1081 cggacccgac ggttgaatca ggtgccgtac acgggggtcg gcttggcggc ctcggcgagc
     1141 aggtccgcca cgatggcgcc gacctcggcc ggctccaacc gtgcgccctt gtcgaaggtc
     1201 gggccgtgca tccagccctg cgcgatgccg atcttgccgc cctcgagctc gaacactcga
     1261 ccggtcacgt gcgacgactg ctccgagccg agccagacaa ccgccgggga gacgttctcc
     1321 ggtgccatcg agtcgaacga cccgtcggtc ggtgccgcca tcatctcggc gaacgcctcc
     1381 tcggtcatac gggtccgagc cgacggggcg agcgcgttgg cggtcacgcc gtagcggccc
     1441 agctcagcgg cctgcacgag ggtcagcgac gcgatgccgc ccttcgccgc ggagtaggcg
     1501 gcctgggcga tcgagcccat gagccccgcg cccgacgagg tgttgatgat ccgacccgac
     1561 accggcttgc cggccttgga ctggtcacgc cagtagccgg cggcgaactt ggccggaagg
     1621 aagtggccct tgaggtgaac ctgcatcacg gcgtcccact catcggcggt ggcgttggcg
     1681 aacatgcggt cgcgaacgat gccggcgttg ttgagcacga cgtcgagtcc gccgaacgtc
     1741 tcgatggcca tgttcaccag gcgctcggca ccctcgaccg aggacacgtc gtcgccgttg
     1801 acgatgccct cgccgccggc ggcggtgatc tcgtccacga cctcctgcgc cggcgacagc
     1861 gaaccgccgg agccgtcgga ctcggcgccg atgtcgttga cgacgacctt ggcaccctcg
     1921 gcggcgaagg cgagggcgtg cgcccgaccg agtccacgcc cggcgccggt cacgatcact
     1981 acccgtccgt cacagatgcc gctcatcgtt ctcctcagtc acattgccga aaggtggtcg
     2041 ggacccgtgc cccgaacgtg tcggacgaca gtttcgcaac aaacctgacg gggtgtcaga
     2101 ttcgcgccct cgtgactagg gtcctgtccc atgcgctacg gggtgacgat cttcgcgacc
     2161 gaccaggcga tgccggctga cgaggttgcc gtcgaggccg aggctcgcgg gttcgactcg
     2221 ctctacctcc ccgagcacac ccacatcccg gtgagtcgcc gcacgcctcc tccgacgggc
     2281 gacgcggagc tggccgagga gtacaagcga acggtcgacc cgctgatcgc gctcgccgcg
     2341 gccgccgccc gcaccacgac gctgcgactg ggtaccggtg tggtgctgcc ggcgcaacgc
     2401 gaaccgatcg tcaccgccaa ggcgatcgcc accttgcaga acctctcggg tggtcgattc
     2461 tgcctggggg tggggttcgg gtggaacgag gacgagatgg ccgaccacgg cgtcgactac
     2521 cgaactcgac gtgcccgctc ccgcgagcac gtgctcgcaa tgcagtcgct ctggaccgag
     2581 gaggtcggcg gcttcgacgg cgagtacgtg aagatcgccc cgagctggtc gtggccgaag
     2641 cccgacgtcg aggtcccggt gctcgtcggt ggcggggccg gcccgaagtt gttctcccac
     2701 attgccgagt acggccacgg ctggatcccg atcggcggcg ccgggctcac cgacgacatc
     2761 ggtcgcttca aggaaacggt ctcggcggcg ggccgagatc ccgaggcgat ggagatcgtt
     2821 ccgttcgggt cgctcccgag cccgtcgaag ctcgaccact tcgagtcgat cggcgtgacc
     2881 gaatgcgtgt tccgtatccc gtctgcaccc cgcgacgtgg tgctcccggt cctcgacgag
     2941 caggccgccc tgctcgcgac gcgacgctag gaagccgagc ggagcggccc gaggaggctc
     3001 agcccgagcg tggtcgatcc gccgtcgggt ggagcgagga acatcatgcc gtcgtcggtg
     3061 aggcccacgg caacggcgcc gttgccggcg taccagtagc cgccgtcgtc gaggtcgatg
     3121 acgtaggcgc gtgccgcgct gtacgcggga tacccgagga acgacacgaa tcgaccgtcc
     3181 gatgagatgt cgaccgagcc accgacgcac aatccgtccc acgagcaccc atcggtgagg
     3241 tcggcggtcg ctccggtcat cgtgtcgagc aaccaccccg tcccgttccc ggcggtcacg
     3301 aggaaccggc cgtcggcggt tgcctcggtg aggaggttcg acgttcgcgc cgggtacagc
     3361 atcgtcccac tcgcctcggt gtctcggaac aactggtcgt caccgctccc gacaccgtcg
     3421 gccgcggcga agtgcgcccc accggtcacg acctggccgt catcggacac attgagcgac
     3481 gtgagggtgg tgatcgaccg ctggaagcgg cgggtcgtgc gttcgccggt cgttcggtcg
     3541 atcatgaaga tacgatcggc gtcgctcccg gcgatggcga gatggcgacc gttggccgaa
     3601 atggcgacgg cctgcgctcc ccggcccacc ggctgtgtga gcgtgtcact cgcgatgtcg
     3661 taggtgaacg tctcggtcac atcgcccacc ggcgtcacgt agacgaccgt ggtcccggcg
     3721 tcgtccatcc ccagactgtt gtaggcccag tactgggtgc cgaacgcggt ggagcgcgag
     3781 atccaccgca ccgaccccgt ggtcgtgtcg agcaggtaga ggtccggtgc gaaatcggtg
     3841 tcagcggggt cgaaggcccg gttggtctcg aaggcgacga agcggcccga cgccgaaacc
     3901 acgaactcga gcgaacagcc cgagcacggc ggatcagcga cggcggcgag caccgtggac
     3961 acggtggcgt tgctcgacgg ctcctcggca ggtaggtcgt cgatcggctc ctggtacgcc
     4021 ggcggatcgg cgtcgaccgg cacgtcgacg accggggccg tcgtcggagg tgggagcttg
     4081 gcccgttcct cggccgcctt gatctcagcc accttgcgga ggccgtcggc cttggagatg
     4141 aggcgcttcc agcg
//
LOCUS       DAOSOA010000300         4152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21539_length_4152_cov_6.007811, whole genome shotgun sequence.
ACCESSION   DAOSOA010000300 DAOSOA010000000
VERSION     DAOSOA010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21539_length_4152_cov_6.007811"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKY13_14480"
     CDS             complement(<1..1006)
                     /locus_tag="PKY13_14480"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT81198.1"
                     /translation="MLRDAARSLWAEPRVPDAPGPVWRDWVLVSALVPVVLLEGVFRE
                     NLVWRVPSVVVALALLPTLPWRRTHPLHSIAAAFGLVTVVEVASLLLVDDWRGLDSTV
                     FVILLVYALLRWGSGRDAVIGLAIVSIPVLLIAVEAQTSDAAEVTLGGVAQGAVFVLL
                     VAAVGASVRYADTARRRGMDQMKLLEREQLARELHDTVAHHVSAIAIRAQAGRVVAST
                     DPAAAVEALVVIEREASRTLAEMRSMVGALRQGEEPDLTPQRGVAQVEDLVTGPGERP
                     QVDVELSGDFDGLRPSVDAAIFRLAQESITNAVRHARRGQRGLADRPGARRRRVHRGP
                     L"
     gene            1138..3549
                     /locus_tag="PKY13_14485"
     CDS             1138..3549
                     /locus_tag="PKY13_14485"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT81199.1"
                     /translation="MPMSAATPRSTSLLGRVAGWCFDHRTKAIGLWLVAVVAVFGAVG
                     AAGSQFSTNAQVPGSGSAAGFAVLAEHFPELGTGGQTGTIVFRAAQGVDDPEVRAAME
                     ELFATVDAGFPDDRGVPQHPGATVVSPYAERGESQIARDGPLAGRVAYAQVNLSSDVD
                     DTESGQLGKAISEHAPEIDGLDVFAGGQYLAVIEPPQTELLGLAFAMIVLILAFGSVL
                     AMGLPIAVALGGVAAGMGMTLLLSNVFTIPNDATVLGIMIGLGVGIDYALFIVSRYRE
                     GLHAGHSARASTVIAMESAGRAVIFAGTTVMISMLGLLLVGVGSVGGMGIGVSATVLA
                     TMFTSTTLLPALLGFARERVELTRWRGLIAAGFTSVALLGLGLGLTPLAASAGGLALL
                     TLLASLAVRPLRSAVPRRNAKPMEATLAYRWSRTVQRNPLRWLVGASVVLVLLAAPVA
                     GLRLGWADDGNFPEGTDTRQAYDLLAEAFGDGFNGPFIITVVGESADSVAALHAALLR
                     TDGVATVTAPIADDPAQPSAYLMSLVATTAPQDEATSTLVRHLRADVIPAATAGTDLD
                     VEVTGGAAIAIDITDYLAKRMLLFFGAVLAVSFVLLLIVFRSLLVPLKAVLMNVLTIG
                     AAYGIVVAVFQWGWAGGLIGIEGGPINPFIPMMLFAVVFGLSMDYEVLLLSRIREEYL
                     RTGDANTSVADGVASTARVITAAAAIMVVVFGSFTLEDMRELKIFGLGLAVAVLLDAT
                     LVRMVVVPATMELLGDRNWWIPTWLDRVLPHFEVEPARPTVPAGDEQLTPAGHAPVAL
                     GRDAR"
     gene            3593..>4152
                     /locus_tag="PKY13_14490"
     CDS             3593..>4152
                     /locus_tag="PKY13_14490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT81200.1"
                     /translation="MSEAPMWDERYSTEEYVFGTDPAAFLTQHEELFVPGSETLVVAD
                     GEGRNSVYLATRGMQVTAMDVSEVGVEKARRLAIDRGATVDFRLADITTWKWAPDAYD
                     MVVAVFIQFLDPEQRAVAFDGMQRTLRTGGRLLLHGYSVDQLAFGTGGPSDPAHLYTD
                     TLLAEAFATMEIEELRSYEAVISEGAG"
BASE COUNT          619 a         1539 c         1348 g          646 t
ORIGIN      
        1 ccagagggcc acgatgcacg cgacgcctcc gagcacctgg gcgatcggcg agaccgcgtt
       61 ggccacggcg ggcgtggcgc acggcattcg tgatcgactc ctgggcgaga cggaagatgg
      121 cggcatcgac ggacggccgc agcccgtcga agtctccgga gagctcgacg tcgacctggg
      181 gccgttcgcc cgggccggtg acaaggtcct cgacctgggc aacgccacgc tgcggcgtga
      241 ggtccggttc ctctccctgt ctcagggcgc cgaccatcga gcgcatctcg gcgagcgtcc
      301 gggatgcctc ccgctcgatg acgaccaggg cttccaccgc tgcggccgga tcggtggagg
      361 ccacgacacg gccggcctgg gcccgaatcg cgatggccga gacgtggtgg gccacggtgt
      421 cgtggagctc gcgtgccagc tgttcccgct cgagcaactt catctgatcc atcccccggc
      481 gacgggccgt gtcggcgtac cggaccgagg caccgaccgc cgccaccagg aggacgaaca
      541 cggcgccttg ggccactccg ccgagcgtga cctcggcggc atcggaggtc tgggcctcga
      601 cggcgatcag cagcaccggg atcgacacga tcgccagccc gatcacggcg tcacggccgg
      661 agccccagcg caacagcgcg tacaccaaca ggatcacgaa gacggtggag tccaggcccc
      721 gccagtcgtc gacgaggagg agcgaggcga cctcgacgac cgtcacgagg ccgaaggccg
      781 ctgcgatgga atgcagcggg tgcgtccgcc gccacggcaa cgtcggaagc agcgccagcg
      841 ccaccaccac ggacggcacc cgccagacca ggttctcgcg gaatactccc tcgagcagca
      901 ccacgggcac cagcgccgag acgagtaccc agtcccgcca gaccgggccg ggcgcatcgg
      961 gaacgcgggg ctcggcccac aacgaacgag ctgcgtcccg gagcacgacc caaccctaac
     1021 gaagtcaccc agccgccgaa tcggccgaaa gtacgagacc gcgcaccgtc cgatcggttg
     1081 gtgccgaatg gccggagtcc cgatgtggcg acggcccgac atcacgacca tgggctgatg
     1141 cccatgtcag cagcaacgcc acgctcaacg tcactcctcg gccgcgtcgc cggctggtgt
     1201 ttcgatcacc ggaccaaggc catcgggctc tggctcgtcg ctgtcgtcgc cgtcttcggt
     1261 gccgtcggcg cggccggctc gcagttcagc accaatgccc aggtgcccgg ctccggcagc
     1321 gctgccgggt tcgccgtgct cgctgagcac ttccccgagc tcggcaccgg cgggcagacg
     1381 ggcacgatcg tgttcagagc agcccagggc gtcgacgatc ccgaggtccg ggccgccatg
     1441 gaagaactgt tcgcaacggt cgacgccgga ttccccgacg accgcggtgt gccgcagcat
     1501 ccgggcgcga ccgtggtctc gccctacgca gagcgcggcg agagccagat cgcgcgggac
     1561 gggcccttgg ccgggcgagt tgcctacgcg caggtcaacc tgtcgtccga cgtcgacgac
     1621 accgagtccg gccagctcgg caaggcgatc agcgagcacg cccctgagat cgacggcctc
     1681 gacgtcttcg ccggcggcca gtacctggcg gtgatcgagc ccccgcagac cgagctcctc
     1741 ggtctcgcct tcgccatgat cgtcctgatc ctggcattcg ggtcggtgct cgccatgggc
     1801 ctcccgatcg ccgtcgcact cggcggtgtc gccgccggca tggggatgac cctgctcctc
     1861 agcaacgtct tcaccatccc caacgacgcc accgtcctcg ggatcatgat cgggctcggc
     1921 gtcggcatcg actacgccct cttcatcgtc tcccgctacc gggaaggcct ccacgccgga
     1981 cactcggctc gtgcatccac cgtgatcgcc atggagtccg ccgggcgcgc cgtgatcttc
     2041 gccggcacta ccgtgatgat ctcgatgctc ggcctcctcc tcgtgggcgt cgggtcggtg
     2101 ggcggcatgg gcatcggtgt ctccgccacc gtgctcgcca ccatgttcac gtccaccacg
     2161 ctcctccctg cactgctcgg gttcgctcgt gagcgggtcg aactcacccg ctggcgcggc
     2221 ctcatcgccg ccggcttcac gtccgtcgcg ctgctcggcc tcggcctcgg gttgacgccg
     2281 ctcgcggcct ccgccggcgg gctcgccctc ctcaccctgc tcgccagcct ggcggtgcgg
     2341 ccgctgcgaa gcgccgtccc tcgacggaac gcgaagccga tggaagcgac cctcgcctac
     2401 cggtggagcc gcacggtgca gcgcaacccg ctccggtggc tggtcggggc gagcgtcgtg
     2461 ctggtgctcc tcgccgcacc ggtcgccggc cttcgcctcg gctgggccga cgacgggaac
     2521 ttccccgagg gcaccgacac acggcaggcc tacgaccttc tcgccgaggc cttcggcgac
     2581 gggttcaacg gcccgttcat catcaccgtc gtgggcgagt ccgccgactc cgtcgccgcg
     2641 ctccacgccg ccctgctgcg caccgacggg gtggccaccg tcaccgcccc gatcgccgac
     2701 gacccggccc agccgagcgc ctatctcatg agtctcgtcg ccaccaccgc tccccaggac
     2761 gaggccacgt ccacactcgt ccgccatctc cgcgccgacg tgatccccgc cgcgacggcc
     2821 ggcaccgacc tcgatgtcga ggtcaccggg ggcgctgcca tcgccatcga catcaccgac
     2881 tacctcgcca agcgaatgct cctcttcttc ggcgccgtgc tggccgtgtc gttcgtgctg
     2941 ttgctgatcg tcttccggtc actcctcgtt ccgctcaagg cggtcctgat gaacgtcctc
     3001 accatcggcg ccgcctacgg catcgtcgtc gccgtcttcc aatggggatg ggcgggcggg
     3061 ctcatcggca tcgaaggcgg tccgatcaac ccgttcatcc cgatgatgct cttcgccgtc
     3121 gtcttcgggc tctcgatgga ctacgaggtg ctcctgctct cccggatccg cgaggagtac
     3181 ctgcggaccg gcgacgccaa tacctcggtc gccgacggcg tcgcctccac cgccagggtg
     3241 atcaccgcag ccgccgccat catggtcgtc gtcttcgggt cgttcacgct cgaggacatg
     3301 cgagaactca agatcttcgg tctcgggctc gccgtcgccg tcctcctcga tgcgacgctc
     3361 gtccgcatgg tggtcgttcc ggccaccatg gagcttctcg gcgaccgcaa ctggtggatc
     3421 ccgacgtggc tcgacagggt cctgccgcac ttcgaggtcg agccggcccg cccgaccgtt
     3481 cctgccggtg acgagcaact gacgccggcc gggcacgctc ccgtcgccct cggccgggac
     3541 gctcgttgaa cgtggatcct ggtgcgtggc agtgtgcgac atggcgacga acatgtccga
     3601 agcaccgatg tgggacgagc ggtactcgac ggaggagtac gtcttcggca ccgatcccgc
     3661 tgcgttcctc acccagcacg aggagctgtt cgttcccggc tccgagaccc tcgtggtcgc
     3721 ggatggggaa ggccgaaact cggtctacct ggcaacgcgt gggatgcagg tcacggcgat
     3781 ggacgtttcc gaggtcggcg tcgagaaagc tcggcgcctc gccatcgacc gcggcgccac
     3841 cgtcgacttc cggcttgccg acatcaccac gtggaagtgg gcacccgacg cgtacgacat
     3901 ggtcgttgcg gtcttcatcc agttcctcga tccggagcaa cgggcggtcg cgttcgacgg
     3961 gatgcagcgc acgctcagga cgggcggacg cctcctgttg cacggctaca gcgtcgacca
     4021 actcgccttc ggcaccggcg gcccctccga tccggctcac ctgtacaccg acacgctctt
     4081 ggccgaggcg ttcgcgacga tggagatcga ggagttgcgc tcctacgagg cggtcatcag
     4141 cgaaggcgca gg
//
LOCUS       DAOSOA010000301         4149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21567_length_4149_cov_3.551050, whole genome shotgun sequence.
ACCESSION   DAOSOA010000301 DAOSOA010000000
VERSION     DAOSOA010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21567_length_4149_cov_3.551050"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKY13_14495"
     CDS             complement(<1..443)
                     /locus_tag="PKY13_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81201.1"
                     /translation="MTAVERDVVDPGIREPGTLPPPARVLGASPATRGPGTLGSGSSP
                     RWSAAVAVLAAVLPVVTDPSGWFVFVAARWAVLAGSTAVLLAGLAATGRLRRLPALGW
                     WASLAGVAVVSAAGSGAARIALFGTTDRRAGAAMWLVHGALFVVGG"
     gene            complement(452..1777)
                     /locus_tag="PKY13_14500"
     CDS             complement(452..1777)
                     /locus_tag="PKY13_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81202.1"
                     /translation="MNDDLDLEARLRSHLRHRADDVDAPDAIGGSLLVREDARRRTAV
                     RHRRRMATASIATAAAVAAGLVVISRDPGTQDVSTDRDQAASDQTGLAAGAATGSSTG
                     SSSGSPTGWTSNYEENADGGLLGLSLRVPSEFDLIGRGSRTARKGSGESAADLAVTAD
                     ATPGAPVRFIVASVQDSRRLQSGTGGTETTILGRHARIISKRAATAITWDMTDTERVL
                     VTTAGVDDTVLTEWLAGLSKDASGRWVFASPGPGGLTVIPTATPTENTAGYQTWSYEN
                     PDADGECAPEGYDLSSSSGGTYDLWGTLADNARWSTSLPVVTSVTLPGTAGATLALVD
                     EYGSVTTLDGDRVLRVSRWNGTSTNAAALASELVGLIFTPTEAERQNVVAPTGIVDPD
                     CETVKGEPGVDAPSRAPEATSPASGPVATTMPPGTELITTSTVSPPPGA"
     gene            complement(1774..2262)
                     /locus_tag="PKY13_14505"
     CDS             complement(1774..2262)
                     /locus_tag="PKY13_14505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT81203.1"
                     /translation="MVEAPTDRAGFDELYRAEFEPMVRLAYLLTGDRETALEVTHDAF
                     VVTCERWSRLDRPGGYLRTAVVNRCRDLGRRQRFRSSAPVPERGVADPDDDLADAIAA
                     LPPKRRAAVVLRFYLDLSEADIAETLGVRPGTVKSLLSRGLAELRSALQEHPPTTSGE
                     PS"
     gene            complement(2437..2877)
                     /locus_tag="PKY13_14510"
     CDS             complement(2437..2877)
                     /locus_tag="PKY13_14510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HOT81204.1"
                     /translation="MMWDEAELRAFAPADRSGLIDLWERCELVRSWNHPGLDIDRKLA
                     VDPEHLLVVSLRGRIVGSVMVGYEGHRGWVNYLAVDPDLRGHGLGRHLMETAERVLAD
                     LGCPKVNLQVRGGNDAALAFYRALGYDVDDAVSLGKRLVVDPHP"
     gene            complement(2874..3872)
                     /gene="moaA"
                     /locus_tag="PKY13_14515"
     CDS             complement(2874..3872)
                     /gene="moaA"
                     /locus_tag="PKY13_14515"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOT81205.1"
                     /translation="MAEQLIDGYGRVHRDLRISVTDRCNFRCTYCMPEDGLQWLPRSE
                     VLTFEEIERVARLLVERFGIDSIRLTGGEPTLRAKLPVLVEKLAALEVDGRPIDLAMT
                     TNAATLAHQVDGLRAAGLRRMNISLDTLRRDRFVELTRRDELDHVLTGIDAAVAAGFE
                     SIKVNAVVMAGVNDDEIVDLATFARDRGVELRFIEFMPLDADAGWQRRRVISRDEIVA
                     TIAAVHPVEPVHRGAAPAERFRYLDGAGHVGVIASVTEPFCESCDRVRLSAEGSLRNC
                     LFALDEFDLRAGLRSGASDDELATIIRSCVAAKWAGHAINQVHFIRPSKSMSQLGG"
     gene            complement(3924..>4149)
                     /locus_tag="PKY13_14520"
     CDS             complement(3924..>4149)
                     /locus_tag="PKY13_14520"
                     /inference="COORDINATES: protein motif:HMM:NF015419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81206.1"
                     /translation="AGEDFTRRTDGKTHFARVVVSAGEGDRYVARSAGGQGSHHLAAM
                     AHANGLAVIEDGDGVAAGDLVDVLLVEPLA"
BASE COUNT          649 a         1451 c         1453 g          596 t
ORIGIN      
        1 cccccgacca cgaacagcgc cccgtgcacc agccacatcg cagctccggc gcgccggtcc
       61 gtcgtgccga acagcgcgat tcgcgcagct cccgagcccg cggccgacac gacagccaca
      121 cccgccaggg acgcccacca gcccagggca gggagacggc ggagccggcc cgtcgctgcg
      181 agaccggcca ggagtaccgc agtcgatccc gcgagcacgg cccacctcgc cgccacgaac
      241 acgaaccatc ccgacggatc cgtgaccacc ggcagcacgg ctgccaggac cgcgacggcg
      301 gcgctccacc gagggctcga cccggagccg agcgtgccgg gcccccgcgt cgccggggac
      361 gcccccagca cccgagcggg cggtgggagc gtccccggtt cgcgaatccc cggatcgacg
      421 acgtcgcgtt cgacggcggt catcggtgcg ctcaggctcc gggcggaggc gacacggtgc
      481 tggtcgtgat caactcggtc ccaggcggca tcgtggtcgc gaccggcccg ctcgctgggg
      541 aggtggcctc aggtgcacgc gagggagcat cgacgcccgg ctcgcccttc accgtctcgc
      601 aatcggggtc cacgatgccg gtgggagcca cgacgttctg ccgctcggcc tcggtcggag
      661 tgaagatcag cccgaccaac tccgaggcca acgcggcggc gttggtcgag gtcccgttcc
      721 accggctcac tcggagaacg cggtcgccgt cgagggtcgt caccgagccg tactcgtcga
      781 ccaaggccag tgtcgcgccg gcggtacccg ggagggtcac cgaggtgacc actggcaacg
      841 acgtcgacca ccgtgcgttg tccgccaacg ttccccagag gtcgtaggtc cccccgctgc
      901 tggagctcag gtcgtagccc tcgggtgcgc actctccgtc cgcgtcgggg ttctcgtacg
      961 accacgtctg gtaaccggcg gtgttctccg tcggcgtcgc cgtcgggatc accgtcaagc
     1021 caccagggcc cggcgacgcg aagacccagc gcccggacgc gtccttgctc aaccccgcga
     1081 gccattcggt gagcacggtg tcgtcgacac cggcggtcgt caccagtacc cgttcggtgt
     1141 cggtcatgtc ccaggtgatg gcggttgcgg cgcgtttgga gatgatgcgg gcgtggcgtc
     1201 cgaggatggt cgtctcggtg ccgcccgtgc cggactgcag tcggcgggag tcctgcacac
     1261 tggcgacgat gaagcgcacg ggcgcgcccg gggtcgcatc cgcggtgacc gcgaggtcgg
     1321 cagccgattc gccggaaccc ttccgagcgg tccgcgaacc acgaccgatc aggtcgaact
     1381 cggacggcac ccggagtgac aaccccagca gtccgccgtc ggcattctcc tcgtagttgc
     1441 tggtccagcc cgtcggcgaa cccgaggacg atccggtgct cgatccggtc gcagcgccag
     1501 cggcgagacc ggtctggtcc gaggcagctt gatcgcggtc ggtgctcacg tcctgggtgc
     1561 ccggatcacg ggagatgacc accagaccgg cagcaacggc agctgccgtc gcgatcgacg
     1621 ccgttgccat gcggcgtcgg tggcgtactg ccgtacgtcg gcgggcgtcc tctcggacca
     1681 gcaggctgcc accgatcgcg tccggcgcgt cgacatcgtc ggcgcggtgg cgtaggtggg
     1741 agcgaagtcg cgcttcgagg tcgaggtcgt cgttcatgag ggttcccctg atgtcgtagg
     1801 tgggtgttct tgcagtgcgg atctcagctc ggcgaggcct cggctgagca atgacttgac
     1861 ggtgcccggc cggacgccga gcgtctcggc gatgtcggcc tcgctcagat cgaggtagaa
     1921 gcgcagcacg accgccgccc gacgcttggg tggcaatgcc gcgatcgcgt cggcgagatc
     1981 gtcgtcgggg tcggcgacgc cccgttccgg cacgggggcc gacgaccgga accgctgacg
     2041 gcgccccagg tcacgacagc ggttcacgac cgccgtgcgc aggtagccgc cgggccggtc
     2101 gagccgcgac catcgctcgc aggtgacgac gaacgcgtcg tgggtcacct cgagcgccgt
     2161 ctcgcggtca ccggtcagca ggtaggcgag gcgaaccatc ggttcgaact cggcacggta
     2221 gagctcgtcg aacccggccc gatcggtggg tgcctcaacc acgacgacgg gtggtcgctc
     2281 gatgatcgtc ggtctgggcg gcggctggga acgggccgtc atggccagtg cttcggatgc
     2341 gtccatatgc cccaacgacg aacgcgaccc ccattcggtt cccggcgtcc tcgccggggc
     2401 gcggtccatc gccgaaccgg actgcgccga cggcgctcac gggtgaggat cgacgaccag
     2461 gcgcttgccg aggctgacgg catcgtcgac gtcgtatccg agcgcgcggt agaaggcgag
     2521 cgccgcgtcg ttgccgccgc gcacctggag gttgaccttc gggcacccga ggtcggcgag
     2581 cacgcgttcg gcagtctcca tgaggtgccg cccgagcccg tgcccacgaa ggtcggggtc
     2641 gacggccagg tagttgaccc agcctcgatg accttcgtac ccgaccatca ccgacccgac
     2701 gatgcgacca cggagcgaga cgacgagcag gtgttcggga tcgacggcga gctttcggtc
     2761 gatgtcgagg ccgggatgat tccagctgcg gaccagctcg caccgctccc agaggtcgat
     2821 gagccccgag cgatcagcgg gcgcgaacgc gcggagttcg gcctcgtccc acatcatcca
     2881 ccgagttgac tcatcgattt ggagggccgg atgaagtgca cctggttgat ggcgtgaccg
     2941 gcccacttgg cggccacgca ggaccggatg atcgtcgcca actcgtcgtc ggacgcaccg
     3001 gatcgcaggc cggcccgcag gtcgaactcg tccagcgcga acaggcagtt gcgcagcgat
     3061 ccctcggcgc tgaggcggac acggtcacag gactcgcaga acggctcggt cacgctcgcg
     3121 atcacgccga cgtggccggc gccgtcgagg tatcggaatc gttccgcagg ggcggcaccg
     3181 cgatgcacgg gctcgaccgg gtggaccgcg gcgatcgtcg ccacgatctc gtcgcggctg
     3241 atgacccggc gccgctgcca cccggcatcg gcgtcgagcg gcatgaactc gatgaagcgc
     3301 agctcgacgc cacgatcacg agcgaacgtc gcgaggtcga cgatctcgtc gtcgttcacc
     3361 ccggccatca cgaccgcgtt caccttgatc gactcgaagc cggcagcgac cgccgcgtcg
     3421 atgccggtga gcacgtggtc gagctcgtca cgtcgggtca gctcgacgaa gcggtcgcga
     3481 cgcagcgtgt cgagcgagat gttcatgcga cgcagcccgg ccgcacgaag cccgtcgacc
     3541 tggtgggcga gcgtcgcggc gttggtggtc attgccaggt cgatcggtcg gccgtcgact
     3601 tcgagggccg cgagcttctc gaccagaacc ggcagcttgg cgcgaagcgt cggttcgcca
     3661 cccgtgaggc gaatggaatc gatcccgaac cgctccacga gcaggcgggc cacccgctcg
     3721 atctcctcga aggtcagcac ctcggaccgg ggcagccact gcaacccgtc ctcgggcatg
     3781 cagtaggtgc agcggaagtt gcaccgatcg gtgaccgaga tacgaaggtc gcggtgcacc
     3841 cgcccgtacc cgtcgatcaa ctgttcggcc atacatcgaa gcgtagagcc cgcccgggcc
     3901 ctcgacccac cgggggcaca acctcaggcg agtggttcga ccagcagcac gtcgacgagg
     3961 tcgccggcag ccacgccgtc gccgtcctcg atgaccgcca gcccattggc gtgggccatc
     4021 gccgcgaggt ggtgggagcc ctgtccgccg gcggatcggg caacgtagcg atcgccctcg
     4081 ccggcggaca ccaccacacg ggcgaagtgg gtcttgccgt cagtgcggcg ggtgaagtcc
     4141 tcgccggcg
//
LOCUS       DAOSOA010000302         4147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21582_length_4147_cov_4.125611, whole genome shotgun sequence.
ACCESSION   DAOSOA010000302 DAOSOA010000000
VERSION     DAOSOA010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21582_length_4147_cov_4.125611"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKY13_14525"
     CDS             complement(<1..332)
                     /locus_tag="PKY13_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81207.1"
                     /translation="MTDLIEIVGAYLLGFVVTAVLWSRSRRIFEGYVFQRHNYRDHSL
                     PTAVGILLPLAAALVVGAHAVIFDVGRSIMGPGATGWAVLGDVGPTTVELAVAFAFLG
                     MLDDIGGLG"
     gene            complement(329..2575)
                     /locus_tag="PKY13_14530"
     CDS             complement(329..2575)
                     /locus_tag="PKY13_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81208.1"
                     /translation="MRRATRQALAGGLLVLWAVVSLAPSASASARQDDTGQRADSSNG
                     RVLIIAAPRLTWAQVDEIRPHYLTQLFERSAVALCSTRTAGSLTRPGDAYLTIGAGNR
                     MGTVIDVDGSVVDRTEPILEGDPTAVYQRTTGKTPTRPILAMGKPAIDHYNEDEQYFG
                     AAAGALAGALGGKGRSMAVIANADRQFGVPRFRQAGLAAMDTTGQIDGGMVSAELLRV
                     DDRAAYGLAVDPEVFMSVFDNAWANDDVVFAELSDLERAEAAREMATPDQGDRQYARA
                     LRAGDDLVGQMLERVDLERDTVIVVGPTPPAAENQLTVFAMAGPGIATGWATSGSTRR
                     DGFVTLTDIAPSVLDRYDIAVPSSMNDTPMSSDADDATVAERTATMIDRADRAVVRDQ
                     AFGPTTVVFIVVLVIDLVLAVLCLARLPRLSGLVRGLALVVLAAPPVTFLLGLVSIAS
                     PAVLGAAVALGAILCAGLASLTRRIDRGLPPVLLFGLLWLVLAVDIATGGQLQINTIF
                     GYSPIVAGRFAGFGNQAFSMIALSSLLLAAVVVERRTSGRTTMGVATTVGVVAWFLVT
                     VVLDGHPAMGSDVGGVLAFVPAATVSLLMFRGTKVRLRLMALIAGGTVAILAAFAALD
                     LSRPAADRTHLGRFVAKLFDGDAGEIIERKIAANLRVLTSVWAWVIPVALVYFMYLTW
                     RPNHTLERLNRHHSQFRAFGVGALTLGVLAMALNDSGVSLPAMMLALVAAYVSYLVID
                     IEGTERAS"
     gene            complement(2572..3687)
                     /locus_tag="PKY13_14535"
     CDS             complement(2572..3687)
                     /locus_tag="PKY13_14535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT81209.1"
                     /translation="MTGSDTHTRSVLELLGPSTGGIRRHVTALGSILTDQGWAVTYAG
                     PAGVLDNLGETAEVVGVPSSFSPAPIARAVRQLRSLRGVDVIHAHGLKAGWVAVLSRR
                     RGTNGRRIPIVLTIHNVVLDEVSGRSAGVLRRLERLLMGRVDRVIAASPGIAEQFAGV
                     VGAGRLSFILPVSPVPHPRRDRSEVRRELGASDDNVLVVVVARLHPQKDLDLFVRAWR
                     TVVSEHPTARAAIVGEGPSRSDLQASIDEAGVAASLRLAGASPDAIEQLAVADVVALT
                     SRWEAVPLVVVEAVQLGRPVVSTRVGVAEELLGDGGTVVDVGDVDAFAAALSRYVGSP
                     DLRQTAGAVSRERGTVIYGADALVRQVARIYEEVLGR"
     gene            complement(3684..>4147)
                     /locus_tag="PKY13_14540"
     CDS             complement(3684..>4147)
                     /locus_tag="PKY13_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81210.1"
                     /translation="RALYATADTKTPAVVNLGLVAVTSIVMLTAVPRLADRDLVTGLA
                     GAYAVGNLGGALALGLVVARRIAATGGGRLGVVAPALRGVAAAVAAGGIGYVCSRSIG
                     WSSKPTAVASLVASSAVAVVAFVGVNWAVGGPAPKVAIATLGAGRDAGADA"
BASE COUNT          670 a         1468 c         1506 g          503 t
ORIGIN      
        1 ccgaggccgc cgatgtcgtc caacatgccg aggaacgcga acgcgacggc gagttcgacg
       61 gtggtggggc ccacgtcgcc gaggaccgcc cacccggtcg cgcccggccc catgatcgac
      121 cggcccacgt cgaagatcac cgcgtgcgca ccgacgacga gggcggcggc aagtggcagc
      181 aggatgccca cggccgtcgg caacgagtgg tcgcggtagt tgtgccgctg gaacacgtac
      241 ccctcgaaga tgcggcggct ccgtgaccac aacacggcgg tgacgacgaa tccgagcaga
      301 tatgcgccga cgatctcgat caggtccgtc atgacgctcg ctccgtgccc tcgatgtcga
      361 tcacgagata gctgacgtac gcggcgacga gtgcgagcat catcgccggc agcgacacgc
      421 cggagtcgtt gagagccatc gccagcaccc cgagcgtgag cgctccgacg ccgaacgccc
      481 ggaactggct gtggtggcgg ttgagtcgct cgagggtgtg gttggggcgc caggtcaggt
      541 acatgaagta caccagcgcg acggggatca cccacgccca cacgctcgtc agcacccgga
      601 ggttggcagc gatctttcgc tcgatgatct ccccggcgtc accgtcgaac agcttggcga
      661 cgaaccggcc gaggtgggtc cggtcggcgg cgggacggct gagatcgagg gcggcgaacg
      721 ccgccaggat cgcgaccgtg ccgccggcga tcagcgccat caaccgcagt cgcaccttcg
      781 tgccgcggaa catcaggagc gacaccgttg cggccgggac gaacgccagg accccaccga
      841 cgtcggaccc catcgcgggg tggccgtcga gaacaacggt gacgaggaac caggcgacga
      901 cgccgaccgt ggtcgccact cccatggtcg tgcggcccga cgtccgccgc tcgacgacca
      961 ccgccgccaa caggagcgac gagagggcga tcatcgagaa ggcctgattg ccgaatccgg
     1021 cgaaacgtcc ggcgacgatc ggcgagtagc cgaagatcgt gttgatctgc agctgtccac
     1081 cggtcgcgat gtcgacggcc aggacgagcc acaagaggcc gaacagcagg accgggggga
     1141 gtccgcgatc gatccggcgg gtgagggacg cgaggccggc gcagaggatc gctccgaggg
     1201 cgacggccgc gccgagcacc gccggtgaag cgatcgacac gaggccgagc aggaacgtca
     1261 cgggcggcgc cgccagcacc acgagggcga gaccccgcac cagtcccgac agtcgcggca
     1321 gacgcgccag gcagagcacg gcgagcacga ggtcgatgac gagcacgacg atgaacacca
     1381 cggtggtggg cccgaacgcc tggtcgcgca ccacggcccg gtcggcgcgg tcgatcatcg
     1441 tggcggtgcg ctccgcgacc gtcgcatcgt cggcgtccga cgacatgggc gtgtcgttca
     1501 tcgacgacgg cacggcgatg tcgtaccggt cgaggacgga cggagcgatg tcggtgaggg
     1561 tgacgaaacc gtcgcgacgg gtcgagcccg acgtggccca gccggtggcg atgccgggac
     1621 ctgccatggc gaacaccgtg agctggttct cggctgcggg cggggtcggc ccgaccacga
     1681 tcacggtgtc gcgttcgagg tcgacgcgct cgagcatctg gccgacgagg tcgtcgcccg
     1741 cccggagcgc ccgcgcgtac tgccggtcgc cctggtccgg cgtcgccatc tcccgggcgg
     1801 cctcggcccg ttcgaggtcg ctcagctcgg cgaacacgac gtcgtcgttc gcccatgcgt
     1861 tgtcgaacac cgacatgaag acctcgggat cgaccgcgag cccgtaggcg gcccggtcgt
     1921 cgacgcgcag gagttcggcc gaaaccatgc caccgtcgat ctgaccggtg gtgtccatgg
     1981 cggcgaggcc ggcctgacgg aaccgcggca cgccgaactg ccggtcggcg ttggcgatca
     2041 ccgccatcga ccggcccttc ccacccagcg cgccggcgag cgcgcccgcc gccgccccga
     2101 agtactgctc gtcctcgttg tagtggtcga tggccggctt gcccatcgcc aggatcggcc
     2161 gggtgggggt cttgccggtc gtgcgctggt agaccgcggt cgggtcgccc tccaggatcg
     2221 gctcggtgcg gtcgacgaca ctgccgtcca cgtcgatgac ggtgcccatc cggttgccgg
     2281 cgccgatcgt gaggtacgca tcgccgggtc gggtgaggct ccctgccgtc cgcgtgctgc
     2341 acaacgccac ggccgaccgc tcgaacagct gtgtcaggta gtgcggtcgg atctcgtcga
     2401 cctgcgccca ggtcaggcgg ggcgcggcga tgatgagcac gcggccgttg gacgagtcgg
     2461 cgcgctgccc ggtgtcgtcc tggcgagccg acgccgacgc cgacggggcg agggacacca
     2521 ccgcccagag gaccagcagt ccgcccgcca gcgcctgtcg ggtcgcgcgt ctcatcggcc
     2581 gagcacctcc tcgtagatcc tggcgacctg acgcaccagt gcatctgcac cgtagatgac
     2641 ggtcccccgc tcccggctga ccgctcccgc ggtctgcctc aggtcgggcg agcccacgta
     2701 gcgggagagg gcggcggcga aggcgtcgac gtcgcccacg tcgacgacgg tcccaccgtc
     2761 gccgaggagt tcctcggcaa cgccgacccg ggtcgagacc accggtcggc cgagctgcac
     2821 cgcttcgacg acgaccagcg gcaccgcctc ccagcgcgac gtgagggcga cgacgtcggc
     2881 cacagccagt tgctcgatcg cgtccggcga ggcgcccgcg agacgcaacg acgcggcgac
     2941 gccggcctcg tcgatcgacg cctggaggtc ggagcgcgac ggcccctcgc ccacgatcgc
     3001 cgcccgagcc gtgggatgct ccgaaacgac ggtgcgccac gcccgcacga acaggtcgag
     3061 gtccttctgg gggtgcagcc gggccacgac gacgacgagc acgttgtcgt cggatgcccc
     3121 gagctctcgc cgcacctcgc tgcggtcgcg ccgagggtgc gggacgggcg ataccggcag
     3181 gatgaacgac aggcggccgg ccccgacgac accggcgaac tgctcggcga tccccggcga
     3241 ggcggcgatc acccggtcga cgcgccccat cagcagccgc tccaggcggc gcaggacgcc
     3301 ggcagatcga cccgagacct cgtcgagcac cacgttgtgg atcgtgagga cgatcggaat
     3361 ccggcggccg ttcgtgccgc ggcgccggct caacaccgcg acccagccgg ccttgagacc
     3421 gtgggcgtgg atcacgtcga cgccgcgcag cgatcggagt tgccgcaccg cccgggcgat
     3481 cggcgccggc gagaacgacg acgggacccc gacgacctcg gcggtttcgc ccaggttgtc
     3541 gagcaccccg gccggtccgg cgtaggtcac cgcccagccc tggtcggtga ggatcgagcc
     3601 gagcgcggtg acgtggcggc ggattccgcc ggtcgacggc ccgagcagct cgaggacgga
     3661 gcgagtgtgg gtgtcggaac cggtcacgcg tccgctccag cgtccctgcc ggcgccgagg
     3721 gtcgcaatcg ccaccttcgg agcgggacca ccgacggccc agttgacgcc gacgaacgcg
     3781 acgacggcta ccgcggacga cgccaccagc gacgcgacgg cggtgggctt cgacgaccaa
     3841 ccgatcgatc ggctgcacac gtagccgatg ccgccggcgg cgacggccgc ggcgacgccg
     3901 cggagcgccg gagccaccac gccgaggcgc ccgccaccgg tggcggcgat ccggcgggcg
     3961 acaacgaggc ccagcgccag cgcaccaccg aggttgccca cggcgtacgc cccggcgagg
     4021 cccgtgacca ggtcacggtc cgcgagccgt gggacggcgg tgagcatgac gatcgacgtg
     4081 actgcgacga ggccgaggtt cacgaccgcc ggcgtcttgg tgtcggcggt cgcgtagagg
     4141 gcgcggg
//
LOCUS       DAOSOA010000303         4135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21666_length_4135_cov_6.811029, whole genome shotgun sequence.
ACCESSION   DAOSOA010000303 DAOSOA010000000
VERSION     DAOSOA010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21666_length_4135_cov_6.811029"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKY13_14545"
     CDS             complement(<1..629)
                     /locus_tag="PKY13_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81211.1"
                     /translation="MHPIERLRYVARSSGAPQDLLMVESARALATFHNDPVALVTACR
                     RVVSRQVTSGGLWWVCSRLLTAAEPLDEVHRIVEAVEDDTTPRALTLALDPDATVAVL
                     GWPSSVADALVRRGSGETLVIDAANEGSSLVRYLNGHGVDAVDVPLGGLGAAVAAADV
                     VVLEACATRTDSVMSVAGSLAAAAVAKVAGVPVWVAVGVGRALPDRPPQA"
     gene            713..1207
                     /locus_tag="PKY13_14550"
     CDS             713..1207
                     /locus_tag="PKY13_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81212.1"
                     /translation="MTTLPADLVLHPLGGKDRTLDEQLKLFHLVAVVLDPYTYESSWL
                     LDTAARILAGFAGADCRVAWVVTADEDGAKAFLGELADQALTFTDPDREFVKALGANE
                     LPAFVHLALDRSVVGIAEGWNPDTWRPIAENLADMMSWTAPTMVKTGDPAPYAGTPAV
                     PARS"
     gene            complement(1220..1996)
                     /locus_tag="PKY13_14555"
     CDS             complement(1220..1996)
                     /locus_tag="PKY13_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT81213.1"
                     /translation="MTTIDVSGLFSLDGQVAVVTGASSGLGRRFARVLHGAGATVVVA
                     ARRADRLDELAAELGDRVVVVPADIADDDSVIALAEAAEAVAGHVDVLVNNAGVGGTT
                     PAELETMERWRSTMAVNLDGLFLLSQQVGLGMIERGHGSIVNIASILGLVGSAPIKQA
                     AYCASKGAVVNLTRELGAQWGRKGVRVNAIAPGWFMSEMTEQDMFGDDSSMEFIRRNA
                     PMGRGGEEHELDGALLFLASNASTYVTGQTIAVDGGWTAR"
     gene            complement(2033..2590)
                     /locus_tag="PKY13_14560"
     CDS             complement(2033..2590)
                     /locus_tag="PKY13_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT81214.1"
                     /translation="MPRDERRAQILGAAATAFAESGFAATSMADVARAAGITKLIVYR
                     HFDSKADLYREILEQVARRLADEWLTVSDVEHSGGHSARVLLAAARAHPDGFRLLFVH
                     APREPEFASYHADFRSIQIDLADRLFAPYVEEALIRDWLCRQAVDHLVAAVLNWIDVA
                     EPSDDERFADLATDGIVAMVRAAAR"
     gene            2700..4067
                     /locus_tag="PKY13_14565"
     CDS             2700..4067
                     /locus_tag="PKY13_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT81215.1"
                     /translation="MRFGIFYEHQLPRPWADGDEQRLIQDALTQVELADRLGFQYVWE
                     VEHHFLEEYSHSSAPEVFLAACSQRTENIRLGHGIIQTAPAYNHPARTAERLAMLDLV
                     SNGRVEFGSGESSSEAELGGFGIDQATKREQWLEGLEVAIRCMTETPFGGHDGTYVQM
                     PPRNVVPKPVQSPHPPLWVACSRRDTIMLAAEKGIGALSFAFIDPPEAERWMVDYEQR
                     LAESCVPVGKAVNPQVACVLPMMIADDESEALRRGIEGANFFGYSLAHFYVFGEHVPA
                     HTDVWQEFVERRDAMGYSPEAAIAAQSEVLGAKIASGDHTGLRGAIGTTDQVREFLRR
                     YEDAGVDQVIFVLQAGRNRHEHIMESLERFGTEILPEFAERDPAHVAERERRFAPHIE
                     AAFARKAELGLDEPRPMPDDYVMKAIPKAMVDRMNSPEATKFLDKVADEGAIGEAQTL
                     GSLLT"
BASE COUNT          659 a         1474 c         1412 g          590 t
ORIGIN      
        1 gcttggggtg gtcggtcagg cagcgcccga ccgacaccga ccgccaccca gacgggcaca
       61 cccgccacct tcgcaaccgc agccgcggcg agcgagccgg cgaccgacat gaccgagtcg
      121 gtgcgggtgg cgcacgcctc cagtacgacg acgtcggcgg ctgcgaccgc agcgccgaga
      181 ccaccgagcg gcacgtcgac cgcgtcgaca ccgtggccgt tgaggtaccg gacgagcgag
      241 ctgccctcgt tggcggcgtc gatgacgagg gtctcaccgc tcccccgccg caccagcgca
      301 tcggcgaccg aggacggcca cccgagcacc gcgaccgtgg cgtcgggatc gagggccagc
      361 gtcagcgccc gaggcgtcgt gtcgtcctcg accgcctcga cgatgcggtg tacctcgtcg
      421 agcggttcgg cggcggtgag cagacgcgaa cacacccacc agagaccacc ggacgtgacc
      481 tgcctcgaca cgacccggcg acacgccgtg accagtgcga cggggtcgtt gtggaacgtg
      541 gccagggccc gggccgactc gaccatgagc agatcctgcg gggcacccga cgaccgggcg
      601 acgtaccgga gccgctcgat gggatgcacg acgggcacac tagcttcggg agggccggcg
      661 gcggacgcac ggatctgcct gtccggggtg ggccgcgcta tctttgcgac ccgtgacgac
      721 gctacctgcc gacctcgtgc tgcacccgct gggcgggaag gatcgaaccc tcgacgagca
      781 gctcaagctg ttccacctgg tggccgtggt gctcgatccg tacacctacg agagctcctg
      841 gctgctcgac accgccgccc gcatccttgc cgggttcgcc ggcgccgact gccgggttgc
      901 atgggtcgtc acggccgacg aggacggcgc caaggcgttc ctcggagagc tcgccgatca
      961 ggccctcacg ttcaccgatc ccgaccgtga gttcgtcaag gcgctcggcg ccaacgagct
     1021 gccggcgttc gtgcacctgg cgctcgaccg gtcggtcgtc ggcatcgccg aggggtggaa
     1081 ccccgacacg tggcgtccga tcgccgagaa cctcgccgac atgatgtcgt ggaccgctcc
     1141 caccatggtc aagaccggcg acccggcgcc ctacgccggc acccccgcag tccccgctcg
     1201 ttcctaatcg gcccatcggt cagcgggcgg tccagccccc gtcgaccgcg atcgtctgac
     1261 cggttacgta ggtcgaggcg ttcgacgcca ggaacaacaa cgcgccgtcg agttcgtgct
     1321 cctcgccgcc gcggcccatc ggtgcgttgc gccggatgaa ctccatcgag gagtcgtcgc
     1381 cgaacatgtc ctgctcggtc atctccgaca tgaaccagcc cggggcgatc gcgttgaccc
     1441 gcacgccttt ccgcccccac tgggcgccca gctcgcgggt caggttgacc accgcaccct
     1501 tggaggcgca gtaggccgcc tgcttgatgg gcgccgagcc caccaggccg aggatcgacg
     1561 cgatgttcac gatgctgccg tggccgcgct cgatcatccc gagcccgacc tgctggctga
     1621 ggaggaacag tccgtcgagg ttgacggcca tcgtcgaacg ccaccgctcc atggtctcga
     1681 gctccgccgg cgtcgtgcca cccacgccgg cgttgttgac cagcacgtcg acgtggccag
     1741 ccacggcttc ggccgcctcg gcgagggcga tgaccgagtc gtcgtcggct atgtcggccg
     1801 gcacgacgac cacacggtcg ccgagttcgg ccgccagctc gtcgaggcga tcggcgcgac
     1861 gagcggcgac gacgacggtg gccccggcgc cgtgcaggac tcgggcgaac cgtcgtccca
     1921 gccccgacga ggcgccggtc accaccgcca cctggccgtc gagtgagaag agcccggaca
     1981 catcgatcgt cgtcatggtc gcgagggtat cgaccgcggc gccgccgagg gttcagcggg
     2041 cagcggcgcg aaccatcgcg acgatgccgt cggtggccag gtcggcgaac cgctcgtcgt
     2101 cggacggttc ggccacgtcg atccagttga ggaccgcagc gaccaggtgg tcgacggcct
     2161 ggcggcacag ccagtcgcgg atcagcgcct cttcgacgta gggagcgaag aggcggtcgg
     2221 cgaggtcgat ctggatcgac cggaagtcgg cgtggtagct ggcgaactcc ggctctcgcg
     2281 gggcgtgcac gaacaggagc cggaacccgt cggggtgcgc cctcgctgcg gcgaggagca
     2341 cccgggcgct gtggccccct gagtgctcga cgtcggacac ggtgagccac tcatcggcga
     2401 gccgccgcgc cacctgctcg aggatctcgc ggtacaggtc tgccttcgag tcgaagtggc
     2461 ggtagacgat cagcttggtg ataccggccg cccgggcgac gtcggccatc gacgtggcgg
     2521 cgaagcccga ttcggcgaac gcggtcgcgg cggcgccgag gatctgtgcc cgccgttcgt
     2581 cgcggggcat gagggtgcga ggctccgttt gtatactccc gagtttacag ctcaggcgtc
     2641 tcgtcccgcc gggacgagac cggtccgtgg gaccaggcga acgtgtccgg ggggacgaca
     2701 tgcgattcgg catcttctac gaacaccagc tgccccgacc gtgggcggac ggcgacgagc
     2761 agcggctcat ccaggacgca ctcacccagg tcgaactcgc cgaccgcctc gggttccagt
     2821 acgtctggga ggtcgagcac cacttcctcg aggagtacag ccactcatcg gctcccgaag
     2881 tgttcctcgc ggcctgctcg cagcgcaccg agaacatccg actcggccac gggatcatcc
     2941 agacggcgcc cgcctacaac caccccgccc gcaccgccga gcggctcgcg atgctcgacc
     3001 tcgtgtcgaa cggacgggtg gagttcgggt cgggcgagtc gtcgtcggaa gccgagctcg
     3061 gcgggttcgg catcgaccag gccaccaagc gcgagcagtg gctcgaaggc ctcgaggtcg
     3121 cgatccggtg catgaccgag acgcccttcg gcgggcacga cggcacctac gtgcagatgc
     3181 cgccgcggaa cgtcgttccc aagccggtgc agtcgcccca tcctccgctg tgggtcgcgt
     3241 gctcgcggcg ggacacgatc atgctcgccg cagagaaggg catcggtgcg ctgagcttcg
     3301 ccttcatcga tccacccgag gccgaacgct ggatggtcga ctacgaacag cgtctcgccg
     3361 aatcctgcgt gcccgtcggc aaggccgtga atccccaggt cgcgtgcgtg ctgccgatga
     3421 tgatcgccga cgacgagtcc gaggcgctcc gccgcggtat cgaaggagcc aacttcttcg
     3481 ggtactccct cgcccacttc tacgtgttcg gcgagcacgt gccggcccac accgacgtct
     3541 ggcaggagtt cgtcgagcgt cgcgatgcca tgggctactc gcccgaggcc gcgatcgccg
     3601 cgcagtccga ggtgctcggg gccaagatcg cctcgggcga ccacaccggc ctgcgcgggg
     3661 cgatcggcac gaccgaccag gtccgcgagt tcctccgccg ctacgaggac gccggggtcg
     3721 accaggtgat cttcgtgctc caggccggcc gcaaccgcca cgagcacatc atggagagcc
     3781 tcgagcggtt cggcaccgag atccttcccg agttcgccga acgggaccct gctcacgtcg
     3841 ccgaacgcga gcgccgattc gctccccaca tcgaggcggc cttcgcccgt aaggctgagc
     3901 tcggtctgga cgaaccgcga ccgatgcccg acgactacgt catgaaggcg atccccaagg
     3961 cgatggtcga ccggatgaac agtcccgaag ccaccaagtt cctcgacaag gtggccgacg
     4021 agggagcgat cggcgaggcg cagaccctcg gctcgctgct cacctgacga tcgggtcagt
     4081 cgccggcgtg accgacggcg aggccgacct gctggccgta cgccagctcg aggtt
//
LOCUS       DAOSOA010000304         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21703_length_4128_cov_4.287994, whole genome shotgun sequence.
ACCESSION   DAOSOA010000304 DAOSOA010000000
VERSION     DAOSOA010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21703_length_4128_cov_4.287994"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKY13_14570"
     CDS             complement(<1..347)
                     /locus_tag="PKY13_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81216.1"
                     /translation="MRRGLLLLATGVMVGCWLLAPATPASSETALPASAVQRTSTTLP
                     VATTVTETTPTTAETVDTVADTPSTAAAPTTVAADSDATTRDDPAATRLRRITYALVG
                     LGGLVLVLNLVFW"
     gene            complement(461..1240)
                     /locus_tag="PKY13_14575"
     CDS             complement(461..1240)
                     /locus_tag="PKY13_14575"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOT81217.1"
                     /translation="MPELIEVELYRQALDATVGRRISAVLTPDPTYARNNLSGDEVRA
                     ALVGTTIESTTRIGKLAVAALDSGHQLGLRFGMTGRLVVDDVAVIDELEYSSGRDDPS
                     WDRFVLHFADGGVARINDPRRFGSVELDPDLTRLGPDATLISALALTAALQSDRSVKA
                     VLLDQSRIAGLGNLLVDESLWRAGIDPARSARSLDDETVRHLRRVIRRTLTQLTRRGG
                     SHRGDLQDERHQGGICPSDGASLTRRTVAGRTTISCPAHQR"
     gene            complement(1233..2423)
                     /locus_tag="PKY13_14580"
     CDS             complement(1233..2423)
                     /locus_tag="PKY13_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT81218.1"
                     /translation="MTDPAPHLDTTAIRAGRGANGSALAPVLWASSAFVTPDSATAHR
                     MATTTRATEFYSRYGNPTVCAFESAMAEMEGGEAARAFSSGMGAISAIVLGLVSSGDH
                     IVTQRQLYGGTQVLFNAVCPRFNIDVTYVDGTDPDAWDAAIIPGRTMLCFAETPANPR
                     LALVDLARFGAIAGPITVVDSTFATPIGQRPLEYGVNLVVHSATKSIAGHNDATIGAV
                     VGERELIDWLWGFAVLHGANASPFDAMNAIRGLRTLPIRHERQSGTAQRIAEFLEAHP
                     AVASVSFPGLASHPQYELARRQLSAPGGLITFDIVGGSVAGRVFVESTTIAQMATSLG
                     GPETLVTHPSTTTHVGLLPAELVDAGITEGTVRMSVGLEHPDDLIADISVALDAAAAA
                     SSSA"
     gene            complement(2420..3664)
                     /locus_tag="PKY13_14585"
     CDS             complement(2420..3664)
                     /locus_tag="PKY13_14585"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOT81219.1"
                     /translation="MASNDQLVATAVDQPAAAGAEPPTGAWLPGMALGRRRLAPVVTD
                     RAFHLEGGGTLPEVTVAYETWGTLAPDASNAVLVCHALTGDSHAAGRAGEGHPGEGWW
                     DGLIGPGRALDTDRYFVVCSNVLGGCQGTTGPASIDPRTGTHYGSTFPVVTIRDMVRV
                     QALLATHLGIERWASVVGGSMGGMQVLEWAAMFPDRVSSILAIATSAAASAQQIAWSM
                     VGRRAILADPAFRGGDYYDAGPGEGPHSGLKLAREIAQIHYRSEGAFADRFGRRLLDR
                     LDGPDGFRLDQRFDVEGYLDYHGDKLVRRFDANSYLRLNKAMDLHDLGRGRGGLAAAV
                     RRMTGPAVIMSIDSDMLYPPYQQEELRDLLVLAGIRCGFYEITSPDGHDGFLIELQQI
                     APILEAFVDASVQDRTLIGGSR"
     regulatory      complement(3678..3793)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            complement(3793..>4128)
                     /locus_tag="PKY13_14590"
     CDS             complement(3793..>4128)
                     /locus_tag="PKY13_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81220.1"
                     /translation="VEGTAARIEAALLAPAATAWAMRRFGGAGMSASALKLAAKQVLE
                     IPLPVNREPWDDAVTVLQTDRERGSEADWLEFGRLMNAAWGIDDDALVEWWLARLPNV
                     ERIVVDPIH"
BASE COUNT          639 a         1367 c         1524 g          598 t
ORIGIN      
        1 ttccagaaca cgaggttgag gacgaggacc agtccaccga ggccgacgag ggcatacgtg
       61 atgcgacgca agcgtgtcgc cgccgggtcg tcgcgggtcg tcgcgtcgga gtcggcagcc
      121 acggtggtcg gggcggccgc cgtcgacggg gtgtcggcca cggtgtcgac ggtctcggcc
      181 gtcgtcggcg tcgtctcggt gaccgtggtg gccacgggca gcgtggtcga cgtccgctgg
      241 acggccgagg cgggcaatgc ggtctccgac gacgctggcg tcgccggcgc aagcagccag
      301 caaccgacca tcactcctgt cgccaggagg agaagacctc gtctcaccag aaccgctcca
      361 aatcgccgtg cgccacgtac gtcgtgccgg gagatactac aaaccgccgt gtgcccgacc
      421 gtgtcatctc ccccgttccg tacgagctcg gacccgaggg tcaccgctgg tgcgccgggc
      481 acgagatcgt cgtgcgtccc gctaccgtcc gacgggtgag cgaagcgccg tcggacgggc
      541 agattccgcc ctggtgccgt tcgtcctgca ggtcgccacg atgcgagccc ccgcgacgcg
      601 tcagctgggt caacgtgcgc cggatcaccc ggcgcaggtg ccgcaccgtc tcgtcgtcga
      661 gcgaccgcgc cgaccgcgcc gggtcgatcc cggcgcgcca gagcgactcg tccacgagga
      721 ggttgccgag cccggcgatc cgcgactgat cgagcaacac ggccttcacc gaacggtcgg
      781 actgcagcgc cgcggtcagt gccaacgcgc tgatcagggt cgcgtcgggc ccgagtcgcg
      841 tgaggtcggg gtcgagctcc acgctgccga atcgtcgcgg gtcgttgatg cgggccacac
      901 caccgtcggc gaagtgcagc acgaaccggt cccacgaggg atcgtcacga cccgacgagt
      961 actcgagctc gtcgatcacg gcgacatcgt cgaccaccag gcgaccggtc atcccgaagc
     1021 ggagaccaag ttggtgcccg gagtcgagtg cagcgacggc gagcttgccg attctggtcg
     1081 tggattcgat cgtcgtgccg acgagggcgg cgcggacctc gtcgccgctg aggttgttgc
     1141 gggcgtaggt gggatcgggg gtgaggactg cgctgatgcg ccggccgacc gtggcgtcca
     1201 gcgcctggcg gtacagctcg acctcgatca gttcaggcac tcgacgaggc cgcggccgcg
     1261 gcgtcgagcg ccacgctgat gtcggcgatc aggtcgtcgg ggtgctcgag gccgaccgac
     1321 atgcgaacgg tgccctcggt gatgccggcg tcgacgagct ccgctgggag caggcccacg
     1381 tgggtggtgg tgctcgggtg ggtcacgagg gtctcgggac cgccgagcga ggtcgccatc
     1441 tgcgcgatgg tggtggactc gacgaagacg cggccggcga ccgaaccacc gacgatgtcg
     1501 aacgtgatga gcccgccggg cgccgacagc tggcggcggg cgagctcgta ctgggggtga
     1561 gacgccaagc ccgggaacga gaccgatgcc acggccggat gagcttcgag gaactcggcg
     1621 atccgctggg cggtccccga ctgccgctcg tggcggatcg ggagcgtgcg gagcccgcgg
     1681 atggcgttca tcgcgtcgaa gggcgacgcg ttggcgccgt gcaacacggc gaaaccccag
     1741 agccagtcga tcaactcgcg ctcgcccacc acggcgccga tggtcgcgtc gttgtgaccg
     1801 gcgatggact tggtcgccga gtggacgacg aggttcacgc cgtactcgag cggccgctga
     1861 ccgatcgggg tggcgaacgt ggagtcgacc acggtgatcg gaccggcgat ggcaccgaag
     1921 cgcgcgaggt cgaccagtgc gaggcgggga ttcgccggcg tctcggcgaa acagagcatc
     1981 gtgcgacccg ggatgatcgc tgcgtcccac gcgtcggggt cggtgccgtc cacgtaggtg
     2041 acgtcgatgt tgaaccgggg gcagaccgcg ttgaacagca cctgggtgcc gccgtagagc
     2101 tgccgttgcg tgacgatgtg gtcgccgctc gacaccagac ccagcacgat cgcggagatc
     2161 gcccccatgc ccgacgagaa cgcccgggcc gcctcgccgc cctccatctc cgccattgcg
     2221 gactcgaacg cgcagaccgt cgggttgccg taccggctgt agaactcggt cgcccgcgtg
     2281 gtcgtggcca tgcgatgggc agtggcggag tcgggggtca cgaacgccga actggcccac
     2341 aggaccgggg ccaacgcgct gccgttggcg ccacgaccgg cacggatcgc cgtggtgtcg
     2401 aggtggggtg cgggatcggt catcgtgaac ctccgatgag ggtgcggtct tggacgctgg
     2461 cgtcgacgaa cgcctcgagg atcggggcga tctgctgcag ctcgatgagg aagccgtcgt
     2521 ggccatcggg gctcgtgatc tcgtagaacc cacaacggat gccggccagg accaggagat
     2581 cccgcaactc ttcctgctga tacggcgggt agagcatgtc gctgtcgatg ctcatgatca
     2641 cggcggggcc ggtcatccgg cggacggcgg cggcgagacc accgcgaccg cgaccgaggt
     2701 cgtggagatc catcgccttg ttgagccgca ggtacgagtt ggcgtcgaat cgccgaacga
     2761 gcttgtcgcc gtggtagtcg aggtagccct cgacgtcgaa gcgttgatcg agccggaatc
     2821 cgtccgggcc gtcgagccgg tcgaggagcc gacgaccgaa ccggtcggcg aacgcaccct
     2881 cggaccggta gtggatctgg gcgatctccc gggccagctt gaggccggaa tggggccctt
     2941 caccggggcc ggcgtcgtag taatcgccgc cccgaaatgc cgggtcggcc aggatggcgc
     3001 ggcggccgac catcgaccag gcgatctgct gggcgctcgc tgccgcagac gtcgcgatgg
     3061 cgaggatgga cgacacccgg tcggggaaca tggcggccca ctcgaggacc tgcattccac
     3121 ccatcgagcc gccgacgacg ctggcccatc gttcgatgcc gagatgggtg gcgagaaggg
     3181 cctgcacgcg caccatgtcg cggatcgtga cgaccgggaa ggtcgacccg tagtgcgtgc
     3241 cggtgcgcgg gtcgatgctc gccgggccgg tggtgccctg gcatccgccg aggacgttcg
     3301 agcagacgac gaagtagcga tcggtgtcga gggcccggcc cggaccgatg agaccgtccc
     3361 accagccctc gcccgggtga ccctcgccgg cccgacccgc ggcgtgggag tcgccggtga
     3421 gggcgtgaca gacgagcacg gcgttcgacg cgtcgggggc aagcgtgccc caggtctcgt
     3481 acgcgacggt gacctcgggg agcgtgccac cgccctcgag gtggaacgcc cggtcggtca
     3541 cgaccggagc gagcctccgg cgccccagcg ccatgccggg cagccacgcg ccggtcggtg
     3601 gttcggcacc ggccgcggcg ggttggtcga cggcggtcgc cacaagttga tcgttgctgg
     3661 ccatcagacc gtggttctcc tggacacttc gttgccggcg aggccgaccg catcccctct
     3721 cggcgaggga ggcaccttgg gtatccgccc caggttgccg gacccacgag gtgggccgct
     3781 caagatgtgg cgtcagtgta tcggatcgac gacgatccgc tcgacattcg ggaggcgcgc
     3841 caaccaccac tcgacgagcg cgtcgtcgtc gatcccccac gccgcattca tgagccgtcc
     3901 gaactcaagc cagtcggcct cggatccccg ctcgcggtcg gtttggagga cggtgaccgc
     3961 gtcgtcccag ggctcccgat tgaccgggag ggggatctcg agcacctgct tggcggccaa
     4021 cttgagggcg ctcgccgaca tgccggcgcc gccgaaccgg cgcatcgccc acgcggtcgc
     4081 agcgggggcg agcagcgctg cttcgatgcg ggcggcggtg ccctcgac
//
LOCUS       DAOSOA010000305         4101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_21888_length_4101_cov_5.112210, whole genome shotgun sequence.
ACCESSION   DAOSOA010000305 DAOSOA010000000
VERSION     DAOSOA010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21888_length_4101_cov_5.112210"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..712
                     /locus_tag="PKY13_14595"
     CDS             302..712
                     /locus_tag="PKY13_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT81221.1"
                     /translation="MAVSFLVQPDRHPLGAHPATRRPQLVGLDGGRSVAAVRRRRVFL
                     VRRCVVGVLAVVAIVLAGSAVSAVSSALSPDVAVPTSFEVAPGDTLWSIAGELGVDAD
                     RREVVQLLSVANGGTVVVPGQHLQIPESVRALGA"
     gene            complement(720..1523)
                     /locus_tag="PKY13_14600"
     CDS             complement(720..1523)
                     /locus_tag="PKY13_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOT81222.1"
                     /translation="MRQLVPPDPLGDTLGGAGIDVAERIASELRAHPDGRPWVFANLV
                     TTIDGAATVDGRSGGLGGPGDRDLFSTLRAEADVIVVGAQTVRDERYRLAREPGPVGR
                     RRRAERGQGARARLCIVTRSSTLDGEPPLLDELPPPGAPAEPWERPLIATIRHAPGAA
                     FDERFDVLELGDDSVDLGRLMEELGRLGVRRVLCEGGPTLLGQLGSAGLVDEWNFTVA
                     PIVAAGDAVRPLHSPHALGQRLRLDRVYAHDDGTLFTRYLTVHGDASLD"
     gene            2033..>4101
                     /locus_tag="PKY13_14605"
     CDS             2033..>4101
                     /locus_tag="PKY13_14605"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOT81223.1"
                     /translation="MSLTDNQVGLGLSRVFTTSDRHPYDEIEWGLRDARITDYRDGSV
                     AFEQTGVEFPLSWSVNASNIVAQKYFRGTLGTPEREWSLRQVIDRVADTIATWGDRDG
                     YFVDDDERDTFVAELKHLLVNQKAAFNSPVWFNIGVPGVPNQGSACFILSVEDSVDSI
                     LEWYREEGVIFKGGSGAGVNLSRVRASTERLAGGGTPSGPVSFMRGADASAGTIKSGG
                     KTRRAAKMVILDVDHPDIEEFVTCKAREERKARVLRDAGFEMDLDGADGFSIQYQNAN
                     NSVRVTDEFMEAVRSDGDWALLARTDGSVQRTVRARDLMRSIATAAWECADPGMQYDT
                     TINRWHTAAVTGRINASNPCSEYMHLDDSACNLASLNLLSFLRDDDTFDEEQFRAAVE
                     VIFVAQEIIVGNADYPTEKIGKTTRRFRQLGLGYTNLGALLMALGHPYDSEDGRAWAA
                     AITALMTGHAYATSARMAARMGPFAGFHENREPMLKVLEMHRAEVVHIDEERVPSSLL
                     GAAQQSWDLACQLGETVGVRNAQASVLAPTGTISFMMDADTTGIEPDLGLVKTKKLVG
                     GGTMSIVNQTVSRALRQLGYGEVELAEIVAYIDEHHSIIGAPHLAPEHLPVFACSMGD
                     NVVHYSGHLKMMAAAQPFLSGAISKTVNTPEETTIEEIEQIYLDGWQLGLKAVAVYRD
                     NCKVGQPL"
BASE COUNT          630 a         1404 c         1385 g          682 t
ORIGIN      
        1 cgctcgccga cggtgcgctc gatgacctcg aggatctccc gttggcgatc ggtgagcggg
       61 tcgagttcgc cgtgtggggc ggagttggca ggtggttggg tgctcatggg ctggctcctg
      121 gttgggaagg gctgacgggt cgtggacccg gacggtcgaa caggcgttcc tgacggtagc
      181 acgtcggaac aaacaagtgt tcgaccttga caccgaacat ccgttcgtgt ccaatggcga
      241 acagttgttc gcatcacccc cggtcatctc actgtcaggg gtccccagga gaatcatcga
      301 catggcagtt tccttcctcg tccagcccga ccgtcacccg ctcggcgccc acccggccac
      361 ccgtcgcccg cagctcgtcg ggctcgatgg tggccgatcg gtcgccgcgg tccggcggcg
      421 tcgggtgttc ctcgtccgtc ggtgcgtcgt cggcgtgctc gccgtcgttg cgatcgtgct
      481 ggcggggtcc gccgtgtcgg ccgtgtcgtc ggcgctgtca cccgacgttg cagtgcccac
      541 ctccttcgag gtggcgcccg gtgacacgtt gtggagcatc gccggcgagc tcggggtcga
      601 cgccgaccgt cgcgaggtgg tgcagctgtt gtcggtggcc aacggcggca cggtcgtcgt
      661 tccgggccag catctgcaga tccccgagtc ggtgcgggcc ctcggggcct gagcgttcgt
      721 cagtcgaggg aggcgtcgcc gtgaacggtc aggtaccgcg tgaacagcgt gccgtcgtcg
      781 tgggcgtaca cccggtcgag gcggaggcgt tgcccgagcg cgtgggggga gtggagcgga
      841 cgcaccgcat cgccggccgc cacgatcggc gccacggtga agttccactc gtcgaccagc
      901 ccggccgagc ccagctggcc gagcagcgtc ggcccgccct cgcagagcac ccggcgcacg
      961 ccgaggcgtc ccagttcctc catcagccga ccgaggtcga ccgagtcgtc gcccagctcg
     1021 agcacgtcga agcgctcatc gaacgctgct cccggagcgt ggcggatcgt ggcgatcagc
     1081 ggccgttccc atggctcggc cggcgctccc ggcggtggca gttcatcgag caggggcggt
     1141 tcgccgtcga gtgtgctcga ccgggtcacg atgcacagcc gtgcccgcgc cccctggccg
     1201 cgttcggctc ggcgacgtcg accgacgggg ccaggttcac gtgccaggcg gtaccgttcg
     1261 tcgcgcaccg tctgcgctcc caccacgatc acgtcggcct cggcgcgcag cgtcgagaac
     1321 aggtctcgat cgccggggcc accgaggccg cccgatcgac cgtccacggt cgcggcgccg
     1381 tcgatggtcg tcaccaggtt ggcgaacacc cacgggcggc cgtcggggtg cgctcggagt
     1441 tccgatgcga tccgttcagc gacgtcgatg cccgcgccgc cgagggtgtc gccgaggggg
     1501 tcggggggaa cgagttgtcg cattgcgttc accccttggc cgtgatccgc cagaaaagtt
     1561 cagatccgta tatccacagg ttgtcccgag gaagtggatg agcggaaggt ggcgagatta
     1621 catctcaact gctggaacac gtggacggtc acggcggttg aacgcactgt ccgtgaggtc
     1681 caccgcccac cgtgagattt tcggcccaac tcgaggcgat catgaccgac gttccccggt
     1741 tattcacacg atgttcccct tttcccccac aggccgttgt tcgtagtgtc ctgggcgcgg
     1801 accgtgacgt gcgcgtttgg attggtggct gcgtctcgtc tcacttggca ggcaggacgg
     1861 gtgctggagg caggaagcga tcgacgacga cccacccggt cgtcccgctc gcaccccgcc
     1921 tccgcccgac gcccacgtca cggtcccagt tctcgcccga cgagcgtccg catcggcgcg
     1981 ctctgccctg cgctgccccc ggtagcgtga acccatcgaa cggagtcgtc acatgtcgct
     2041 gaccgacaac caggtcggcc tcggcctcag ccgggtcttc accacgtcgg atcgtcaccc
     2101 ctacgacgag atcgagtggg ggctccgcga tgcgcgcatc accgactatc gcgacggctc
     2161 ggtggcgttc gagcagaccg gggtcgagtt cccgctgtcg tggtcggtga acgcgtccaa
     2221 catcgtcgcc cagaagtact tccggggcac gctcggcacc cccgagcgcg agtggtcgct
     2281 ccgtcaggtc atcgatcgtg tcgccgacac gatcgccacg tggggcgacc gagacggcta
     2341 cttcgtcgac gacgacgagc gtgacacctt cgtcgccgag ctcaagcatt tgctcgtcaa
     2401 ccagaaggcg gcgttcaaca gcccggtctg gttcaacatc ggtgtgccgg gtgtgccgaa
     2461 ccagggctcg gcctgcttca tcctgtccgt cgaggactcg gtcgactcga tcctcgagtg
     2521 gtaccgcgag gaaggtgtga tcttcaaggg gggctccggc gccggagtga acctgtccag
     2581 ggtgcgggcc tcgaccgagc gtctcgccgg cggcggcaca ccgtccggcc ccgtcagctt
     2641 catgcgcggc gccgatgcgt cggcgggcac gatcaagtcc ggcggcaaga cgcgtcgcgc
     2701 cgccaagatg gtcatcttgg acgtcgacca tcccgacatc gaggagttcg tcacctgcaa
     2761 ggcccgcgag gagcgcaagg cccgcgtcct gcgcgacgcc ggcttcgaga tggatctcga
     2821 cggcgccgac gggttcagca tccagtacca gaacgccaac aactcggtgc gtgtgaccga
     2881 cgagttcatg gaggcggtcc gctccgacgg cgactgggcc ctcctggccc gcaccgatgg
     2941 ctcggtccag cgcacggtcc gtgcccgtga cctcatgcgg tcgatcgcga ccgcggcctg
     3001 ggagtgtgcc gaccccggga tgcagtacga cacgaccatc aaccggtggc acaccgcggc
     3061 cgtgaccggt cgtatcaacg ccagcaatcc ctgcagtgag tacatgcacc tcgacgactc
     3121 ggcctgcaac ctggccagtc tcaacctgct gagcttcctc cgcgacgacg acacgttcga
     3181 cgaggagcag ttccgtgccg cggtcgaggt catcttcgtg gcccaggaga tcatcgtcgg
     3241 caacgccgac tacccgaccg agaagatcgg caagaccacg cggcggttcc gccagttggg
     3301 gctcggctac acgaacctcg gtgcgctgct gatggcgctc gggcacccct acgactccga
     3361 ggacggtcgt gcgtgggccg cagcgatcac cgcactcatg accggccacg cctacgcgac
     3421 gtcggctcgc atggcggctc gcatgggtcc gttcgccggg ttccacgaga accgcgagcc
     3481 gatgctgaag gtgctcgaga tgcaccgggc cgaggtcgtc cacatcgacg aggagcgggt
     3541 cccgtcgtcg ctgctcggcg ccgcccagca gtcgtgggat ctggcctgcc agctcggcga
     3601 gacggtcggc gtgcgcaacg cgcaggcgag tgttctcgct cccaccggca cgatctcgtt
     3661 catgatggat gccgacacga ccggcatcga gcccgatctc ggcctggtca agacgaagaa
     3721 gctcgtcggc ggcggcacca tgtcgatcgt caaccagacc gtcagccgtg cgctccgcca
     3781 gctcgggtac ggcgaggtcg agctcgccga gatcgtcgcc tacatcgacg agcaccactc
     3841 gatcatcggg gccccgcacc tcgctccgga gcatctgccg gtgttcgcct gctcgatggg
     3901 cgacaacgtc gtgcactact ccgggcacct caagatgatg gcggcggcgc agccgttcct
     3961 ctcgggggcg atcagcaaga ccgtcaacac gcccgaggag acgacgatcg aggagatcga
     4021 gcagatctac ctcgatgggt ggcagctcgg gctcaaggcc gtcgccgtct accgcgacaa
     4081 ctgcaaggtc ggccagccgc t
//
LOCUS       DAOSOA010000306         4073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_22094_length_4073_cov_5.307367, whole genome shotgun sequence.
ACCESSION   DAOSOA010000306 DAOSOA010000000
VERSION     DAOSOA010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22094_length_4073_cov_5.307367"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2947
                     /locus_tag="PKY13_14610"
     CDS             <1..2947
                     /locus_tag="PKY13_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81224.1"
                     /translation="LPNAFVKRAHDPSFAAAANAALGAIEGASFYLFCHDDVELGPGT
                     VQAMVEEAFRSNAGIVGAKLVDWDDPEHLRSVGGSIDKFGFAWPIAEPNELDQSQHDA
                     VREAFVVSSAAMLVRCDLFADLGGFSTDIDGWGEELDLCWRARIAGGRTVVMPAAVVR
                     HRERAELGDPSGRSPHVALRHQARIMLACYSPMQLLRIAPQAILLGIVDLLSSLVRGR
                     VGVAGDIAGAFGWNLLHLPRTLGIRGRVKRSRRVADDEIRRMQIKGSVRVTSFLRRYA
                     SGDRSMTATLAAAARGLPRHRDDDQSDGLWPFAVFVTSVVVLLVGSRTLIGSGIPAVR
                     EFVPIGDPAHLLSEWWSGWRTPGLGAADGTPTLALVAGIGNWITFGSEGFVRTVLILA
                     PLPLGAWSTWRLLRGAASPAARAAALLAYLVNPLPYNAIAEGRWQALVAYGAAPALLG
                     RVARAGEWSPFDRANPAPGSTQRQIVGMGLLLAAATTVAPVLALFAVVLCAVVVVTMA
                     VTTRSGRPLHAVVVTAGALAVSAMIHLPWTWSVLTASNRWSVLVGADPGRRTPLSLRS
                     AVLFDTGSHGGWVSLGLVLAVVVAVLISTGDRFRWSTVSAVLIVVSFAAVVVAGRLAP
                     GVALPAPELLLSLAAVGVSLAVALGVEAFRADVVGGAFGARQLLSVIALVAVVVSSVP
                     LVVDFLNGRWLAPKSDIDVALAPIRERSGVPSDRTLWIGDADALSTQGWELADGTKFA
                     LTDGVRPTFSSLFPPAMGPGERRLRDVLNETTDGGTGRLGAELAEFGVRYVVVLQRLA
                     PLPYGQTRFPIDPELDDHLNEQFDLVRVEVAPGLDVFENVASLPVRSVVETVPDGRGA
                     AQAEALVATRSEVAAWKADGHPPVSYDGDLPSDRSLMVLSTRDDGWSLDVDGESVAPE
                     PVLGWASRYDVQAGGPAELAYSTPASQVVLHLIQLAFVATLPWARRRRISEFRVRSTR
                     SRGTGTVES"
     gene            2944..>4073
                     /locus_tag="PKY13_14615"
     CDS             2944..>4073
                     /locus_tag="PKY13_14615"
                     /inference="COORDINATES: protein motif:HMM:NF040160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HOT81225.1"
                     /translation="MIRRVGLVVVLGLVVLGLFRLDDPHATPSPEPSPVVGDIVDDDA
                     GTTTWYCAAAPEAAEGAEHQIVITALTDDVTARITGYGPDTGSAVAVSTPVAARSVHV
                     VSLAEVGPGLGGATVEIEGGPATVAHRLIGSGVEDQSECTDEASDRWYFASANTELGA
                     GEVDSTPRLWLLNPFPTDASVDVRVTFDNSVRIPSKLRGIIVPAGSSRMVDLNEPVPR
                     RAQFAFSVEARGGRVVAELAQTVPGRGLRVQPGVPTTAASWILADSFGGANVTDQVHV
                     YNPSSSPVVAMVSVIPNQVDPASFPEPFVLEIPARRFGTVDLGVEARVPPDSLRWIRV
                     DTVDGPGVVVTQVETITGAGGDGTTGTRPAVAGGLGSSAGASA"
BASE COUNT          477 a         1449 c         1451 g          696 t
ORIGIN      
        1 cctccccaac gcgttcgtga agcgtgcaca cgacccgtcg ttcgctgccg cagcgaacgc
       61 agcgctcggt gcgatcgaag gcgcgtcgtt ctatctcttc tgccacgacg acgtggaact
      121 cggccccggc accgtgcagg cgatggtcga ggaagccttc cggtcgaacg ccggcatcgt
      181 cggcgccaag ctcgtcgact gggacgaccc cgagcacctc cgcagcgtcg gtggatcgat
      241 cgacaagttc ggcttcgcgt ggccgatcgc cgagccgaac gagctggacc agtcccagca
      301 cgacgccgtc cgcgaggcct tcgtcgtctc gagtgccgcc atgctcgtcc gctgcgacct
      361 gttcgccgac ttgggcgggt tctcgaccga catcgacggc tggggcgagg agctcgacct
      421 gtgttggcgg gctcgaatcg ccggaggccg caccgtcgtc atgcccgcag cggtggttcg
      481 tcaccgcgaa cgagcggaac tcggcgaccc gagcggccgc agcccgcacg ttgcgctgcg
      541 tcaccaggct cgcatcatgc tcgcctgcta cagcccgatg cagctcctcc gcatcgctcc
      601 gcaggcgatc ctgctcggca tcgtcgatct cttgagttcc ctcgtgcgtg ggcgcgtcgg
      661 cgtcgcgggc gacatcgccg gggcgttcgg ctggaacctc ctgcacctcc cgcgcaccct
      721 cggcatccgg ggtcgggtca agcgatcccg ccgcgtcgcc gacgacgaga tccgccgcat
      781 gcagatcaag ggcagcgtgc gggtcacgtc gttcctgcgc cgctacgcgt cgggcgatcg
      841 atcgatgacc gccacgctcg ccgcagcggc gcggggattg ccgcgccacc gtgacgacga
      901 ccagtccgac ggtttgtggc cgttcgcggt gttcgtcacg agcgttgtcg tgttgctggt
      961 cgggtctcgc acgttgatcg ggagcggcat ccccgccgtg cgggagttcg tcccgatcgg
     1021 tgaccccgcc cacctcctct cggagtggtg gtcgggctgg cgcacccccg ggctcggcgc
     1081 tgccgacggg acgccgacgc tcgccctggt cgcgggcatc ggcaactgga tcacgttcgg
     1141 ttcggaaggc ttcgtccgca cggtgctgat cctcgccccg ttgcccctcg gcgcctggtc
     1201 gacgtggcga ctgctccggg gcgcggcctc gccggcggcg cgtgccgcag cgctgctggc
     1261 gtacctggtg aacccgctgc cctacaacgc gatcgccgag ggccgttggc aggcgctcgt
     1321 ggcctacggc gcggcacctg cgctcctcgg tcgggtcgcc cgggccgggg agtggtcgcc
     1381 gttcgaccga gccaacccgg cgcccgggtc cacacaacgt cagatcgtgg gcatgggtct
     1441 gctgctggcc gctgccacca cggtcgcgcc cgtcctggcg ctcttcgcgg tcgtcctgtg
     1501 cgcggtggtc gtcgtcacga tggcggtgac gacgcggtcg ggccgtccgc tccacgccgt
     1561 ggtcgtcacc gccggcgccc tcgccgtgtc ggcgatgatc cacctgccgt ggacctggtc
     1621 ggtcctgacc gcgtcgaacc gctggtcggt gctggtcggc gccgacccgg ggcggcggac
     1681 gccgctgtcg ctccgcagcg ccgtgctctt cgacactggt tctcacgggg gctgggtcag
     1741 cctcggcctc gtgctcgcgg tcgtggtggc cgtcctgatc tcgaccggcg accgcttccg
     1801 gtggtcgacc gtgtcggccg tgctcatcgt ggtgtcgttc gccgccgtcg tcgtcgccgg
     1861 gcgcctcgcc ccgggggtgg cgctccccgc gccggagctg ctgttgtcgc tcgcagcggt
     1921 cggggtgtcg ttggcggtcg cgttgggcgt cgaggcattc cgcgccgacg tcgtcggggg
     1981 agcgttcggc gctcgtcagc tcctctcggt gatcgcactc gtcgcggtcg tggtgtcgtc
     2041 ggtgccgttg gtggtcgact tcctcaatgg tcggtggctc gcccccaaga gcgacatcga
     2101 cgttgcgctg gcgccgatcc gcgaacggtc aggcgtgccc tcggaccgga cgctgtggat
     2161 cggtgacgcc gacgcactgt ccacccaggg gtgggagctc gccgatggca cgaagttcgc
     2221 gctgaccgac ggcgtacgtc cgaccttctc gtcgttgttc ccgccggcca tgggtccggg
     2281 tgagcgccgt ctgcgcgacg tgctgaacga gacgaccgac ggtggcaccg gtcgactggg
     2341 ggccgaactg gccgagttcg gcgtccgcta cgtcgtggtg ctccagcgcc tggccccgct
     2401 gccctacggc cagacccgct ttcccatcga tcccgagctc gacgaccacc tcaacgagca
     2461 gttcgatctc gttcgcgtcg aggtcgcccc cggtctcgac gtgttcgaga acgtggcgag
     2521 tctgccggtc cgttccgtcg tggagaccgt gcccgacggt cgcggcgcgg cacaggctga
     2581 ggcgctggtc gcgacccggt ccgaggtggc cgcttggaag gcggacggcc acccgcccgt
     2641 ctcttatgac ggcgacctcc cgtcggatcg atcgctgatg gtgctgtcga cccgagacga
     2701 cggctggagc ctcgatgtcg acggcgagtc ggtggcgccc gagcccgtgc tcgggtgggc
     2761 atcgcggtac gacgtgcagg ccggtggccc cgcggagctc gcgtattcga cgcctgcgtc
     2821 acaggtcgtc ctccacctga tccagctcgc gttcgtggcc accctgccct gggcacggcg
     2881 ccggcggatc tcggagttcc gagtacggag tacccggtca cgagggacgg ggacggtgga
     2941 gtcatgatcc gacgcgtcgg actcgtggtc gtcctgggtc tcgtggtgct cggcttgttc
     3001 cgactcgacg atcctcacgc gaccccgtcg cccgagccgt caccggtcgt cggcgacatc
     3061 gtcgacgacg atgcgggtac caccacgtgg tactgcgccg cggcccccga ggcagccgag
     3121 ggcgcagagc accagatcgt catcaccgcg ctgaccgacg acgtcacggc gcggatcacc
     3181 ggctacgggc ccgacaccgg gtcggcggtc gcggtctcga cgccggtcgc tgcacgttcg
     3241 gtgcacgtcg tgtccttggc cgaggtgggc cccggcctcg gcggcgccac ggtcgagatc
     3301 gagggtggtc cggcgacggt cgcccatcgg ttgatcggca gcggggtcga ggatcagtcc
     3361 gagtgcaccg acgaggcgtc cgaccgctgg tacttcgcgt cggccaacac cgagctgggc
     3421 gcgggcgagg tcgactccac gccccgactg tggttgctca acccgttccc gaccgatgcc
     3481 agcgtcgatg tgcgggtcac gttcgacaac agcgtccgca tcccgtccaa gctccggggc
     3541 atcatcgtgc cggccgggag ttcgcggatg gtcgatctca acgaacccgt gccccgtcgg
     3601 gcccagttcg ccttcagcgt cgaggcccgt ggcggccggg tggtcgccga gctggcgcag
     3661 acggttccgg gccgagggct tcgggtccag ccgggtgtac cgacgacggc ggcgtcctgg
     3721 atcctggccg actcgttcgg gggcgccaac gtgaccgatc aggtgcacgt ctacaacccg
     3781 tcgtcgagcc cggtcgtcgc catggtgtcg gtgatcccca accaggtcga cccggcgtcg
     3841 ttccccgagc cgttcgtgct cgagatcccg gcccgacgct tcggcacggt cgatctcggg
     3901 gtcgaggccc gggtcccgcc cgactcactg cgttggattc gcgtcgacac ggtcgacggc
     3961 cccggtgtgg tcgtcacgca ggtcgagacg atcaccggtg ccggcggcga cggcaccacg
     4021 ggtacccggc ccgcggtcgc gggcgggctc ggttcgtcgg cgggagcgtc ggc
//
LOCUS       DAOSOA010000307         4043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_22326_length_4043_cov_4.449097, whole genome shotgun sequence.
ACCESSION   DAOSOA010000307 DAOSOA010000000
VERSION     DAOSOA010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22326_length_4043_cov_4.449097"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /locus_tag="PKY13_14620"
     CDS             <1..1632
                     /locus_tag="PKY13_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT81226.1"
                     /translation="PVDIPTELAALDRARDATKVKLAKLSTIDNEAADEYSAAFMDHM
                     VGVTIDKLRQELVVFGRIDDELPWRVGLYGIDSGGEQLVIDWRAPFAEGFYQATLDDP
                     RGLRRRVSYVGSITDLLVEDFVDGGVSGASPLMAELSRSRGTEMRAAVATLQSEQDRL
                     VRLDPAAQLVLRGGPGTGKTVVALHRAAWLVYNDRRITSDRFLVIGPSDRFLRFVSAV
                     LPTLGESRITQTTFDRHLGSSHPAGSDPRWLDVLDRLEASLPQPTTVRSSGRWIPEDE
                     IAALCERVLALSVPWRERRKIFLGRVASQLEINAKEATRVGGAVMPAVSTTAAWKRIT
                     SKASLEALGAPDDLIDAWLGPRGRDKPSDGALLDEVKARFEGVTTRYAHVVVDEAQDL
                     TLFQLRAVMRRSDGLTLVGDDAQRSVAAGLGLRKVAEMLGTPLEQMDIAYRMSAEIAD
                     WLNAHAERNGIDSVRLVGIRPNGIAVVDAPGTDAETHRARLAERWENVALIERSDVWS
                     HKGVEYDGVVVDTAGLSAHEVYLAASRAAHELVIV"
     gene            1697..2437
                     /gene="pepE"
                     /locus_tag="PKY13_14625"
     CDS             1697..2437
                     /gene="pepE"
                     /locus_tag="PKY13_14625"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOT81227.1"
                     /translation="MELLLLSNSTNYGESMYAHAAAAFATVTDGRTVTFVPYALADWD
                     DYADRAIEAFGAFGIEAQSAHRSSAPERAILEAEVIMMGGGNTFRLLDALIGLGVVEE
                     LGMRVRTGLTRYMGASAGTNVACPSIRTTNDMPICRPPTFTALRLVPFQINLHYVDPD
                     PDTTFMGESRDERISEFLEESDCPVLAMYEGTWLRVSGTHATVTGPARLFERSGCADF
                     ADGVDVSELMHLRPSFDTGRRPVSSRLR"
     gene            2553..3644
                     /locus_tag="PKY13_14630"
     CDS             2553..3644
                     /locus_tag="PKY13_14630"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HOT81228.1"
                     /translation="MSHQPTAVVYCEGNFGDIDGKTANGLVRHSETYRILSVIDSRHT
                     GEDSGAVLGDGPNGIPVVTDLAAAIALTGSAPDMLIYGMAPASGMLSADERLVLLGAM
                     EQGIGIVNGLHEFLSDDPEFAAAAAAYGVIMLDVRRPRDKKDLRMFSGRIDGVTCPRI
                     AVLGTDGAIGKRTTATILVRALNDRGIRAVLVGTGQTGLIQGAKYGVALDAIPCQFCS
                     GEMESAVVDAFEGEHPDVIVIEGQGALSHPAYLSSAFILRGSRPDAVVLQHAPGRTML
                     GDFPAVPMPTPESEIRLIETFADTKVIGLTINHENMTDAEVTAAITMYELELGIPATD
                     ALGRPPERLVDMVLEAFPQLSSRMASSAS"
     gene            3641..>4043
                     /locus_tag="PKY13_14635"
     CDS             3641..>4043
                     /locus_tag="PKY13_14635"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT81229.1"
                     /translation="MTAPRLEVRLDQLGHNAATLVGRLARRGVAVTAVTKATLGSPEI
                     ARELLRAGVSTIGDSRIENLERLRHAGIDAPTLLIRSPMISQVDRVVASADVSCNTEI
                     DVIDRLSLAARGQRRRHGVILMVELGDLREGI"
BASE COUNT          617 a         1417 c         1373 g          636 t
ORIGIN      
        1 cccgtcgaca tcccgaccga gctcgctgcg ctcgaccggg cccgggatgc gacgaaggtg
       61 aagctggcga agctgtcgac gatcgacaac gaggcggccg acgagtattc ggcggcgttc
      121 atggaccaca tggtcggcgt cacgatcgac aagctgcgcc aggagctcgt ggtgttcggc
      181 cgaatcgacg acgagttgcc ctggcgcgtc gggctgtacg gcatcgattc cggcggcgag
      241 cagctggtga tcgactggcg ggccccgttt gccgagggct tctaccaggc aaccctcgac
      301 gaccctcgtg gtcttcgccg gcgtgtgagc tacgtggggt cgatcaccga tctgttggtc
      361 gaggacttcg tcgacggggg agtgtcgggg gcgtcgccgc tcatggcgga gctgtcgcgg
      421 agtcgcggca ccgagatgcg cgccgcggtc gccaccttgc agtccgagca ggaccgcctc
      481 gtccggctcg atcccgcagc gcaactggtg ctgcgcggtg gcccgggcac cggcaagacg
      541 gtcgtggcgc tgcaccgggc ggcgtggctg gtctacaacg accgacgaat cacgagcgac
      601 cggttcctcg tgatcggccc gagcgatcgg ttcctccgct tcgtgtcggc ggtgctcccg
      661 acgctcggcg agtcgaggat cacccagacc acgttcgacc ggcacctggg ctcgtcgcat
      721 cccgccggtt ccgatccgcg ttggctcgac gtcctcgatc gcctcgaagc gagcctgccg
      781 cagccgacga cggtgcgttc gtcgggccgt tggatccccg aggacgagat cgccgcgctg
      841 tgcgagcgtg tgctcgcctt gtcggtgccg tggcgtgagc gtcgcaagat cttcctcggg
      901 cgggtggcgt cgcaactcga gatcaacgcg aaggaggcaa cccgcgtcgg cggagcggtg
      961 atgcccgccg tgagcacaac ggccgcatgg aagcggatca cgtcgaaggc atcgctcgag
     1021 gcgctcggtg cgcccgatga cctcatcgat gcctggctcg gaccccgagg ccgcgacaaa
     1081 ccctccgacg gcgcactcct cgacgaggtg aaggcccggt tcgagggggt gaccacgcgc
     1141 tacgcccacg tggtcgtcga cgaggcgcag gacctgacgc tgttccagct ccgggcggtg
     1201 atgcgccgct ccgatggact caccctcgtc ggcgacgacg cccagcggtc ggtcgccgcg
     1261 ggcctcggcc tgcgcaaggt ggccgagatg ttgggcacac cgctcgaaca gatggacatc
     1321 gcctaccgca tgtccgccga gatcgccgat tggctcaatg ctcacgccga gcgcaacggc
     1381 atcgactcgg tacggctggt cggcatccga ccgaacggca tcgccgtggt cgacgcacct
     1441 ggcaccgacg ccgagacgca tcgggcccgc ctggccgaac gctgggagaa cgtggcgctg
     1501 atcgagcggt ccgacgtctg gagccacaag ggcgtcgagt acgacggggt cgtcgtcgac
     1561 acggccggcc tcagcgccca cgaggtgtac ctcgcagcgt cgcgcgccgc ccacgagctc
     1621 gtcatcgtct gaccccgcaa gcggcgccgg gaccgcgatg cgggtccgcg acatcgcggc
     1681 cgcgagactc gcgttgatgg aactgctgct gctgtcgaac tcgacgaact acggcgagtc
     1741 gatgtacgcc cacgcggccg ctgccttcgc caccgtcacc gacggccgga ccgtcacgtt
     1801 cgtgccgtac gcgcttgccg actgggacga ctacgccgac cgggccatcg aggcgttcgg
     1861 agcgttcggc atcgaggcgc agtcggcgca tcgatcgtcg gcgccggagc gggccatcct
     1921 cgaggccgag gtcatcatga tgggcggcgg caacacgttc cggctgctcg acgcgctgat
     1981 cggcctcggt gtggtggagg aactcggcat gagggtgcgg accgggctga cccgctacat
     2041 gggcgcgtcc gccggcacga acgtcgcctg tccgagcatc cgcaccacca acgacatgcc
     2101 gatctgccgc ccgcccacgt tcaccgcgct ccgcctcgtg ccgttccaga tcaacctcca
     2161 ctacgtcgat cccgaccccg acaccacgtt catgggcgag agccgcgacg agcggatctc
     2221 ggagttcctc gaggagagcg actgcccggt cctcgcgatg tacgagggca cgtggctccg
     2281 ggtgtccggt acccacgcca cggtcaccgg ccccgcccgg ctgttcgaac ggtccggttg
     2341 tgccgacttc gcggacggtg tggacgtgtc ggagctgatg cacctccgcc cctcgttcga
     2401 caccgggcgt cggccggtgt cgtctcgact ccggtagggt cacgggtacc accgcgcttc
     2461 gcggggacct cttcatcacc ggtgcacggc ctccacggtc gcagcgcggg tccttcggtc
     2521 gcctcctctg agcccaactc gaggagttca ccatgtccca ccaacccacc gccgttgtct
     2581 attgcgaggg caacttcggc gacatcgacg gcaagaccgc caacggtctg gtccgccatt
     2641 ccgagacgta ccgcatcctc tcggtgatcg acagtcgtca caccggcgag gacagcggtg
     2701 ccgtgttggg cgacgggccg aatggcatcc ccgtcgtcac cgatcttgcc gcagccatcg
     2761 ccctgaccgg gtcggcgccc gacatgctca tctacgggat ggctcccgcc agcggcatgc
     2821 tgtcggcgga cgaacggctc gtgctgctcg gtgcgatgga gcaggggatc ggcatcgtca
     2881 acggcctcca cgagttcctg tccgacgatc cggagttcgc cgcggctgct gccgcgtacg
     2941 gcgtgatcat gctcgacgtg cgtcggcctc gcgacaagaa ggacctccgc atgttcagcg
     3001 ggcggatcga cggggtcacc tgcccccgca tcgccgtgct gggcaccgat ggcgcgatcg
     3061 gcaagcgcac caccgccacg atcctcgtgc gggcgctcaa cgaccggggc atccgtgccg
     3121 tgctggtcgg caccggtcag accggcctca tccagggagc gaagtacggc gtcgcactcg
     3181 acgcgattcc gtgccagttc tgctccggtg agatggagtc cgctgtcgtc gacgcgttcg
     3241 agggcgaaca ccccgacgtc atcgtgatcg agggtcaggg agcgctcagc cacccggcct
     3301 acctcagttc ggcgttcatc ctccggggca gccgtcccga cgccgtcgtg ctgcagcacg
     3361 cgccgggccg gacgatgctc ggtgacttcc ccgccgtgcc gatgccgacg cccgagagcg
     3421 agatccgcct gatcgagacc ttcgccgaca cgaaggtgat cggcctgacg atcaaccacg
     3481 agaacatgac cgacgccgag gtcaccgccg cgatcacgat gtacgagctc gagctcggca
     3541 tccccgcgac cgacgccctc ggccgcccac ccgagcgact ggtcgacatg gtgctggagg
     3601 ccttccccca gctgagttcg aggatggcgt ccagcgcgtc gtgaccgcgc cgcggttgga
     3661 ggtccggctc gaccagctcg gccacaacgc cgcgaccctc gtcgggcgtc tcgcccggcg
     3721 aggtgtcgcc gtcaccgcgg tgacgaaggc aaccctcggt tcccccgaga tcgcccgtga
     3781 gctcctgcgg gcgggagtgt cgacgatcgg cgactcgcgc atcgagaatc tcgaacggct
     3841 ccggcatgcc ggtatcgacg cgccgacgct cctgatccgg tcgccgatga tcagccaggt
     3901 cgatcgggtg gtcgccagtg cggacgtgag ctgcaacacc gagatcgacg tgatcgaccg
     3961 gttgtcgctc gctgcccgag gacaacgccg acgccacggc gtgatcctga tggtggagtt
     4021 gggtgacctc cgcgagggca tcc
//
LOCUS       DAOSOA010000308         4012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_22574_length_4012_cov_7.149103, whole genome shotgun sequence.
ACCESSION   DAOSOA010000308 DAOSOA010000000
VERSION     DAOSOA010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22574_length_4012_cov_7.149103"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKY13_14640"
     CDS             <1..529
                     /locus_tag="PKY13_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT81230.1"
                     /translation="DDVLRRTHPPRNAPRLADGAPVHTRDGGARLDPARLDDLCPDWA
                     DRETFVCGPTPLIEFVTDHWDAAGALERVHLERFTLDLPAPADPATGDTVTATFATTG
                     SIATAPVGATLLATAEAAGIDAPYGCRTGVCHTCSTRLVSGCTTDVRDGRVSEAGTHV
                     QLCVSTPLTDVALDL"
     gene            563..1666
                     /locus_tag="PKY13_14645"
     CDS             563..1666
                     /locus_tag="PKY13_14645"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HOT81231.1"
                     /translation="MTALIDRPLTHDAPLDPIDPAGMDLDAFAAELDAIRDEILASRG
                     QADADYIRRVIRLQRRLEIGGRAALMAGVLPPAWLAGVAMLSLSKILENMEIGHNVMH
                     GQWDWMRDPDIHSTTWEWDNVCPSPQWKHSHNYMHHQWTNVVGMDNDIGYGILRIDPN
                     KPWERWNLAQPLIFVTLATLFEYGVGFHDLEADVSDGEKVTFERIKPKLFEIGRKIRR
                     QVAKDYVAFPALALPLGVPSAVSVATGNLAANVVRNLWSFAVIFCGHFPDGVEIFPRG
                     SAENETRGHWYRRQVLGSANFEGGPLMHLMTGNLDHQIEHHLFPDLPSNRYVEVAPKV
                     REVCERHGVAYNTGSFARQFGSVVRKVLRLSLP"
     gene            complement(1653..3101)
                     /locus_tag="PKY13_14650"
     CDS             complement(1653..3101)
                     /locus_tag="PKY13_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOT81232.1"
                     /translation="MFLRSDDESHAALTGAVAAVTAEVNRWAREGAWSGIDGDELAAA
                     VEALDPLPASGVAFADVVDEVGRVVLANGVRPSDPATVAHLHSPTLITAAATEVAIGM
                     TNQSMDSYDQAPAATLVEDRLVRRLAEVCGLPATASGVMTAGGTASNVLGLVLAREAA
                     ARRRGLDVATDGLPGEARRWRIVASDAAHFSVARAAMLMGLGRAAVVSAPTDASGAID
                     PVRFDEVVAATTAAGDEVIAVVGTAGTTDLGAIDPLGHLADRAAALGAWFHVDAAVGG
                     AFVLSTAMRPRLAGIERADSVTVDLHKLWWQPISASALLVADEASFGLIRRHSEYLDR
                     EEDAETGVLNLVGRSLDTSRRFDALKALVSLRTVGRAGLASMLEHLVDTASTVAASID
                     AHREFELLAPTTTVMVVFRWVGDGTLTDAELDRANTDVQRQLFDDGRAVIGRSTIGGR
                     VALKFTVVNPDLTVDDLVALLDAIADEAFREG"
     gene            3243..>4012
                     /locus_tag="PKY13_14655"
     CDS             3243..>4012
                     /locus_tag="PKY13_14655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HOT81233.1"
                     /translation="MYQAEPIDLAPGSLLWRWAGDQRLGFTGLATGILQLMHPGLGAG
                     VVEHSAFFTEPWDRIQRSVPEIIGVIYDEDGDATGHRVRDYHKRIKGTDAQGRRYSAL
                     KPETFWWAHATFQYSVEQLADRFDNHRLTPDEREQLYREGVEWYRRYGVSMRPVPPDY
                     RSFVTEWNRYCTDVLEMTPAAERAIDMALHEKVEHMPGLPWWSLPIQREILTPLLRLT
                     AIGGLPVSVRTKFGIPFGLSDTLQLKALELWVRQNWRFV"
BASE COUNT          643 a         1474 c         1315 g          580 t
ORIGIN      
        1 ggacgatgtt ctccgccgaa ctcacccgcc tcgcaacgcg ccacgactgg ctgacggtgc
       61 accggtgcac acccgcgacg gcggtgcccg actcgatccg gcccggctcg acgacctctg
      121 tcccgactgg gcggaccgcg agacgttcgt gtgcggcccg acgccactca tcgagttcgt
      181 cacggaccac tgggatgccg ccggcgcgct cgaacgcgtc catctcgagc gattcaccct
      241 cgaccttccg gcgcccgccg atcccgccac cggcgacacg gtcaccgcga cgttcgcgac
      301 caccggcagc atcgccacag ctccggtcgg cgcaacgctg ctggccacgg ccgaagccgc
      361 cggcatcgac gccccctacg ggtgccgcac cggggtgtgc cacacgtgct cgacccgcct
      421 cgtgtcgggc tgcaccaccg acgtccgaga cggtcgcgtc agcgaggccg gcacccacgt
      481 gcagttgtgc gtgtcgaccc cgctcaccga cgtcgcactc gacctctagc ccccgcctcc
      541 cccaccccga caaggacccc tcatgaccgc actcatcgac cgtccactca cccacgacgc
      601 cccgctcgac cccatcgacc ccgccgggat ggacctcgac gcgtttgccg ccgagctcga
      661 cgccatccgc gacgagatcc tcgccagtcg cggccaggcc gacgccgact acatccgccg
      721 ggtcatccgc ctccaacgcc ggctcgagat cggcgggcgc gccgccctca tggccggcgt
      781 gctgccgccg gcgtggctcg ccggtgtcgc gatgctcagc ctctccaaga tcctcgagaa
      841 catggagatc gggcacaacg tgatgcacgg ccagtgggac tggatgcgcg atcccgacat
      901 ccactcgacg acgtgggagt gggacaacgt ctgcccctcg ccccagtgga agcactccca
      961 caactacatg caccaccagt ggacgaacgt cgtgggcatg gacaacgaca tcggctacgg
     1021 catcctccgc atcgatccca acaagccgtg ggaacggtgg aacctcgccc agccgctgat
     1081 cttcgtgacg ctcgcgacgc tgttcgagta cggcgtcgga ttccacgacc tcgaggccga
     1141 cgtgagcgac ggcgagaagg tcaccttcga gcgcatcaag ccgaagctgt tcgagatcgg
     1201 ccgcaagatc cgtcgccagg tcgccaagga ctacgtggcg ttccccgcgc ttgcactccc
     1261 cctcggcgtg ccctccgcgg tgtcggtcgc gaccggcaac ctcgccgcca acgtcgtgcg
     1321 gaacctgtgg tcgttcgccg tgatcttctg tggacacttc cccgacgggg tcgagatctt
     1381 tccccgcggc agcgccgaga acgagacccg agggcactgg taccggcggc aggtcctcgg
     1441 atcggccaac ttcgagggcg gccccctcat gcacctcatg accggcaacc tcgaccacca
     1501 gatcgagcac cacctcttcc ccgacctgcc gtcgaaccgc tacgtcgagg tcgcgccgaa
     1561 ggtgcgcgag gtctgcgaac ggcacggggt tgcgtacaac accggctcgt tcgcccgtca
     1621 gttcggcagc gtcgtgcgga aggtgctccg gctcagcctt ccctgaaggc ctcgtctgcg
     1681 atcgcgtcga gcagggccac gagatcgtcg acggtcaggt ccgggttcac caccgtgaac
     1741 ttgagggcga cccgtccgcc gatcgtcgag cggccgatga cggcgcgacc gtcgtcgaac
     1801 agctgccgct ggacatcggt gttggctcga tcgagctcgg cgtcggtcag cgtgccgtcg
     1861 ccgacccagc ggaacacgac catgaccgtc gtggtcgggg ccagcagttc gaactcccgg
     1921 tgcgcgtcga tcgatgcagc gacggtcgat gccgtgtcga cgaggtgctc gagcatcgaa
     1981 gccaggccgg cccggccgac ggtgcggagc gacaccagtg ctttgagcgc atcgaagcgg
     2041 cgcgacgtgt cgagtgagcg gccgacgagg ttgagcacgc cggtctcggc gtcctcctcg
     2101 cgatcgaggt actcgctgtg tcggcggatc aatccgaacg aggcctcgtc agcgaccagc
     2161 agggcgctgg cgctgatcgg ttgccaccac aacttgtgga ggtcgacggt gaccgagtcg
     2221 gcccgttcga tgccggcgag ccgggggcgc attgcggtgc tcaggacgaa ggccccgccg
     2281 acggcggcgt cgacgtggaa ccacgctccg agtgcggcgg cgcgatcagc gagatgcccg
     2341 agcgggtcga tcgcccccag gtcggtggtg cccgccgtgc cgacgacggc gatgacctcg
     2401 tcgccggcgg cagtggtcgc tgcgacgacc tcgtcgaacc tcaccgggtc gatggcaccg
     2461 ctcgcatcgg tgggtgcact cacgaccgcg gcccgcccga gccccatgag catggcggcc
     2521 cgggcgacgc tgaagtgcgc cgcgtccgac gccacgatcc gccagcgccg agcctcgccc
     2581 gggagcccgt cggtggccac gtcgaggccc cgccgtcggg cagccgcttc gcgggcgagc
     2641 accagcccga gcacgttcga cgccgtccct cccgctgtca tgacacccga cgcggtcgcg
     2701 ggcagaccgc acacctcggc cagccggcgc accaggcggt cctccacgag ggtggccgct
     2761 ggcgcctggt cgtaggagtc catcgactgg ttggtcatcc cgatggcgac ctcggtggcc
     2821 gcggcggtga tgagcgtggg gctgtgcagg tgcgcgaccg tcgcgggatc cgacggacgc
     2881 acgccgttgg cgagcacgac gcgacccacc tcgtcgacga catcggcgaa ggcgacgccg
     2941 gaggcgggca gcggatcgag cgcctcgacg gcggcggcca gctcgtcgcc gtcgatgccc
     3001 gaccatgcac cctcgcgggc ccagcggttg acctcggccg tgacggcggc gacggccccg
     3061 gtcagggcgg cgtgggactc gtcgtcggag cggaggaaca cagccgggag cgtagatcgc
     3121 ctcgccagac accggttttg tcctgcactt caatcggcaa accgatcgaa atgcaggaca
     3181 aaaccggaag gggttcgggc gggccattga cgtgacggga cagtggtacg aaactcgtgt
     3241 ttatgtacca ggcggaaccg atcgatctcg cacccggctc gctcctgtgg cggtgggccg
     3301 gcgaccaacg gttggggttc acgggcctcg ccaccggcat cctccagctc atgcacccgg
     3361 gcctcggcgc cggggtcgtc gagcactcgg ccttcttcac cgaaccgtgg gaccgcatcc
     3421 agcgatcggt gcccgagatc atcggggtca tctacgacga ggacggcgac gccaccggcc
     3481 accgggtccg cgactaccac aagcgcatca agggcaccga tgcccagggt cgccgctaca
     3541 gcgccctcaa gcccgagacg ttctggtggg cgcacgccac gttccagtac tccgtcgagc
     3601 agctcgccga ccgcttcgac aaccaccgcc tgacccccga cgaacgcgag cagctctacc
     3661 gcgagggagt cgagtggtac cgccgttacg gcgtcagcat gcgtccggtt ccacccgact
     3721 accggtcctt cgtcaccgag tggaaccggt actgcaccga cgtgctcgag atgaccccgg
     3781 cagccgaacg ggccatcgac atggcgctcc acgagaaggt cgagcacatg cccggcctcc
     3841 cgtggtggag cctgcccatc caacgcgaga tcctgacccc gctcctccgc ctcaccgcca
     3901 tcgggggcct gccggtgtcg gtccgcacca agttcggcat cccgttcggg ctgagcgaca
     3961 cgctccagct caaggcgttg gagttgtggg tccgacagaa ctggcggttc gt
//
LOCUS       DAOSOA010000309         3992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_22723_length_3992_cov_6.720599, whole genome shotgun sequence.
ACCESSION   DAOSOA010000309 DAOSOA010000000
VERSION     DAOSOA010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22723_length_3992_cov_6.720599"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..1614)
                     /locus_tag="PKY13_14660"
     CDS             complement(277..1614)
                     /locus_tag="PKY13_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81234.1"
                     /translation="MSDVGTLATLPDPTLVEEKNSISFRFGRRTWLAIGALTLITGVL
                     AMTATFFLPKQFVVSRSLLIMSGNNPNDNDTLIRAFEEIMADKGFAAEIKAKTGLKLP
                     AETIKAMISPSRPPAAATIKVSVRSTKMAEADKVSAAIVPTLTEVVESGQRNLPIEER
                     ISGPIVQEIFNRPIREEEFVPWWVGLFSGASIAFLIAFVVAAFRQYRKPVISSARDVG
                     DALDLPVLARIMAVGDGRSTNPQDAVLGMLSAIERLGASGPIHRLVVVGPDSDVERSK
                     LVLALGCAIARNFDQPVALIDADLENASLTKLIGASDEPGLAECLTGDLRVDQTLLRL
                     ENGHTPQLLDGMVPPSGMIRVMPAGINRGGSLLRMRSNLHQVLGAMSGKYVVVVDGPQ
                     VPGPVPVTQLLSMADATIVVVTEGGTSIRDARFTGNALRSNTTNPVGAVVLRK"
     gene            1848..1949
                     /locus_tag="PKY13_14665"
     CDS             1848..1949
                     /locus_tag="PKY13_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOT81235.1"
                     /translation="MGSLIKKRRKRMRKKKHRKLLRRTRHQRRARGK"
     gene            complement(1997..2740)
                     /locus_tag="PKY13_14670"
     CDS             complement(1997..2740)
                     /locus_tag="PKY13_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT81236.1"
                     /translation="MITLSAPAKLTVSLRLTGLRSDGMHLIDAEMVSVDLFDTLHISE
                     GTGVYMAGPAAGDLVGRTDGTDLVSRALRMIGRDVRVDVDKQIPAGAGLGGGSSDAAA
                     ILRWGGFDDLDAAAKLGADVAFCLAGSRARVTGIGEIVEPLPPVDRTFTLLTPPVHCS
                     TPVVYRRWDAMGGPTAPDGAVDPNDLTDAAVASFPEIGEWRDRLEEATGLTPRLAGSG
                     STWFVEGDYPGDGRRVVHTIGGSAVVPER"
     gene            complement(2751..3584)
                     /gene="rsmA"
                     /locus_tag="PKY13_14675"
     CDS             complement(2751..3584)
                     /gene="rsmA"
                     /locus_tag="PKY13_14675"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOT81237.1"
                     /translation="MVDPIPALLTRTSLLEVMARYGLEPHRTLGQNFVVDQNTIRKIV
                     RLAGVESGDAVVEIGPGLGSLTLGLLEAGAEVTAVEIDRRLIEPLTEITGGRARILAA
                     DAMEVDWSTVVGDRPHTVVANLPYNVATPVVLRLLDEAPQVTRMLVMVQREVGERLAA
                     GPGSKAYGIPSVKVGYWATARLVGTVGAEVFHPQPRVTSALVEIVRREHPVHAVDPVV
                     LFDLVRAGFGQRRKTLRRALGDRVTDAQLEAADIDGRRRAEELDVAEWCRLTEAVSPL
                     R"
     gene            complement(3577..>3992)
                     /locus_tag="PKY13_14680"
     CDS             complement(3577..>3992)
                     /locus_tag="PKY13_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81238.1"
                     /translation="PTTTAVTTTTGAPKDGDEPPPVQVPIDPNWVAKGWSRYSDSDDE
                     TLSRVNRNNMAGTTVPLPTTAPKPTTTVPGGKTTTTSAPSRTTVPPATNPAPTAPPPT
                     APPPTSPPATEPPPVTDPPVTTPPAAAVDPAATGG"
BASE COUNT          662 a         1379 c         1354 g          597 t
ORIGIN      
        1 gagtcaccac cgcgtcgtcg accgacttca gcgccgcctc ttcactccca accgccgtga
       61 cccgcaacat tcaccagctc ctgatatcga cattgcgcgt tcgggtcgac ggactacccc
      121 ccgacgacca cgagctgcac acaccccagt actggtgaag ctaagcacgc cctcaagcca
      181 aactcaaggg tcacaatggc tcatcgaaca ccgttcacca acgattcgcg aggcatttgc
      241 accgtcatga catcgacctg gacggccgat gacgccctac ttacggagga cgacggcgcc
      301 gacggggttc gtggtgttcg accgcagggc gttgcccgtg aaccgggcat cgcggatcga
      361 cgtcccaccc tcggtcacca cgacgatcgt ggcatcggcc atcgacagca gctgggtgac
      421 cggcaccgga cccggcacct gcgggccgtc gacgacgacc acgtacttgc ccgacatcgc
      481 cccgagcacc tggtggaggt tggagcgcat gcgaaggagg gacccaccgc ggttgatgcc
      541 ggcgggcatg acccggatca tgccggacgg cggaaccatg ccgtcgagga gctgtggggt
      601 gtggccgttc tccaggcgga ggagcgtctg gtcgacccgg aggtccccgg tcaggcactc
      661 ggcgaggccg ggctcgtcgg aggcgccgat cagcttggtg agtgaggcgt tctcgaggtc
      721 ggcgtcgatc agcgcgacgg gctggtcgaa gttgcgggcg atggcacacc cgagcgccag
      781 cacgagcttg gagcgctcga cgtcggaatc ggggcccacg accaccagac gatggatcgg
      841 gcccgacgct ccgagccgct cgatcgccga gagcatgccg agcacggcgt cctggggatt
      901 ggtgctgcga ccgtccccga ccgccatgat ccgggcgagc accggcaggt cgagcgcatc
      961 gccgacgtca cgcgccgagc tgatgaccgg cttgcggtac tggcggaacg ccgcaacgac
     1021 gaaggcgatg aggaacgcaa tcgaagcgcc gctgaacagg ccgacccacc agggcacgaa
     1081 ctcctcctca cggatcgggc ggttgaagat ctcctggacg atcgggccag agatgcgctc
     1141 ctcgatgggc aggttgcgct gaccgctctc gacgacctcg gtcaacgtcg gaacgatcgc
     1201 cgccgacacc ttgtccgcct cggccatctt cgtggaacgc accgacacct tgatggtggc
     1261 ggcggccggc gggcggctcg gggagatcat cgccttgatc gtctccgccg gcagcttgag
     1321 cccggtcttc gccttgatct cggcggcgaa gcccttgtcg gccatgatct cctcgaacgc
     1381 gcggatgagc gtgtcgttgt cgttggggtt gttgcccgac atgatcagga gcgaccggga
     1441 caccacgaac tgcttgggca ggaagaacgt tgcggtcatc gccagcacgc cggtgatcaa
     1501 ggtcagcgca ccgatggcga gccaggtacg gcgtccgaac cggaagctga tcgagttctt
     1561 ctcctcgacc agggttggat ccggcagcgt tgccagcgtc ccaacgtcac tcatgcgctc
     1621 attcccatcc gctgctcctg cgagggcgcc caccggcgca agctcacgaa gagtctctgt
     1681 tcgttcactc tgcgccgatc agcacgagtg caggtacaag cgaaaggcta ccgacgcagg
     1741 ctcgaaagcg aaaggcacgg actcgtgaac aaacggtgca gagacgcgcg gcgaatgtcg
     1801 ttccgggttc gcactctcgg agacggctcg ctaaagtgcc tcccgccatg ggttcactga
     1861 tcaagaaacg ccggaagcgg atgcggaaga agaagcaccg caagctcctt cgccgcactc
     1921 gccaccaacg ccgagctcgc gggaagtagc cctcggtcgc tgggcatctc cgctcggcac
     1981 ggtcgcgtcg ttcctgctaa cgctccggaa ccacggccga tccaccgatc gtgtgtacga
     2041 cccgtcggcc atcgcccggg tagtcaccct ccacgaacca ggtcgatcca ctgccggcga
     2101 gccgcggggt cagccccgtc gcttcctcga ggcggtcccg ccactccccg atctcgggga
     2161 acgaggcgac tgccgcgtcg gtgaggtcgt tcggatcgac cgcgccatcc ggtgcggtcg
     2221 gcccacccat cgcgtcccac cgccggtaga cgaccggcgt cgagcaatgc accgggggcg
     2281 tcaacagggt gaacgtgcgg tcgaccggcg gcaacggttc gacgatctcg ccgatcccgg
     2341 tcacccgcgc ccgcgaccca gcgaggcaga aggccacgtc cgcgccgagc ttcgcggcgg
     2401 catcgaggtc gtcgaagccg ccccaccgga ggatcgccgc cgcgtcgctg ctgccacccc
     2461 ccaggccggc gccggcgggg atctgcttgt ccacgtcgac acggacgtca cggccgatca
     2521 tacggagcgc gcggctcacg agatcggtgc catcggtccg tcccaccagg tcgccggcgg
     2581 ccggtccagc catgtagacg ccggtaccct cggagatgtg gagcgtgtcg aacaggtcga
     2641 ccgacaccat ctcggcgtcg atgaggtgca tcccgtccga acgcaggccc gtgaggcgga
     2701 ggctgacggt gagcttggcg ggcgccgaga gcgtgatcat gctgcggacc ctaccgaagg
     2761 ggcgagaccg cctcggtcag ccggcaccac tcggccacgt cgagctcctc ggcacgccgc
     2821 cgaccgtcga tatcggccgc ctcgagctgg gcgtcggtga cacgatcgcc gagcgctcga
     2881 cgcaacgtct ttcggcgttg gccgaacccc gcacgcacga gatcgaacag caccaccgga
     2941 tcgacagcgt ggacgggatg ctcgcggcgg acgatctcga cgagcgccga ggtcacccgc
     3001 ggctgggggt ggaacacctc ggcgccgacg gttcccacca gacgagcagt cgcccagtac
     3061 ccgaccttca ccgacgggat gccgtacgcc ttggaacccg gtccggcggc cagccgctcc
     3121 cccacctccc gctgcaccat caccaacatc cgcgtcacct ggggggcctc gtcgaggagg
     3181 cgcaggacca ccggagtcgc gacgttgtag gggaggttcg cgacgacggt gtgggggcgg
     3241 tcacccacga ccgtcgacca gtcgacctcc atggcatcgg cggcgaggat cctcgcccga
     3301 ccgcccgtga tctcggtcaa cggctcgatg agacgccggt cgatctcgac agcggtcacc
     3361 tcggcgccgg cctcgagcag tcccagcgtc agcgagccga ggcccggccc gatctcgacg
     3421 acggcgtcac ccgactccac ccccgccaac cggacgatct tgcggatcgt gttctgatcg
     3481 acgacgaagt tctgtcccag cgtgcggtgc ggctcgaggc cgtaccgggc catgacctcc
     3541 aggagggagg tgcgggtgag cagcgccgga atcggatcaa ccaccggttg ccgccgggtc
     3601 gaccgcagcg gcgggaggag tcgtgaccgg cggatcggtg accgggggcg gctccgtggc
     3661 cggcggcgac gtcggagggg gcgcggtcgg gggcggcgcc gtcggagccg ggttggtcgc
     3721 cggcggcacg gtggtccgac tcggcgcgct cgtggtcgtc gtcttgcccc ccggcaccgt
     3781 cgtcgtgggc ttcggggcgg tggtcggcag cgggaccgtc gtgccggcca tgttgttgcg
     3841 attgacgcgg ctcagcgtct cgtcgtcgct gtcgctgtac cgactccaac ccttggcgac
     3901 ccagttcgga tcgatgggca cctgcacggg aggcggttcg tcgccgtcct tcggtgctcc
     3961 cgtggtggtg gtcacagcag tggtggtcgg ag
//
LOCUS       DAOSOA010000310         3991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_22732_length_3991_cov_3.638211, whole genome shotgun sequence.
ACCESSION   DAOSOA010000310 DAOSOA010000000
VERSION     DAOSOA010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22732_length_3991_cov_3.638211"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKY13_14685"
     CDS             complement(<1..623)
                     /locus_tag="PKY13_14685"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81239.1"
                     /translation="MTTYLGYRDGGADTAWGRFFEPEMQPLHPHVVDALARGPQPEPL
                     LTDRAGVAEFVRTASEPTEDGYAVCRDGSIRVAVRTPMPGVTPAMWEWWFGWHGSDSR
                     RYKLWHPRAHVSAEWADGAGEGDFVGRTSIVREYLGSTFFHAAIRFVPPAEFGIDHVP
                     DGELTVCARLGDATRPVDGGWFVHHVRPTADGAEMRSRFWMGGSHIAL"
     gene            complement(620..1990)
                     /locus_tag="PKY13_14690"
     CDS             complement(620..1990)
                     /locus_tag="PKY13_14690"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT81240.1"
                     /translation="MNLALPDGVAYERGDDGYEAARRATVWNARLPDRFPDVIVQAHT
                     EADVVAAVRFAAEHSHRVGVRSGGHSWAANHVRDGGVLLDVSRLDALAVDPATMTATV
                     GPGRKGHEMCAALADQGLFFPAGHCKGVAVGGYLLQGGYGWNGRVLGPACQSVIGVDV
                     VTADGEQVFADADHHADLYWSARGAGPGFFGVVTRFHVRLYPKPDAIGMSLYAYPIEC
                     LDEVFTWAREIADDVDRRVEMQILMSREFAELDIHDPAIIIASPVFADSDEEAEAALA
                     LLGTCPAIDRALIAIPYAPAGLMEWYDAVMHNYPDGHRYATDNMWTSAAAADLLPGLH
                     RIVESLPPAPSHCLWLNWGSSPERQDMAYSMEDEVYIALYGVWTDAADDDLYADWARS
                     NMASMEDLATGIQLADENLGRRPAPFVLPQNMERLDAARAAYDPDGRFHAWMGRVGAN
                     GSGPAS"
     gene            2059..2841
                     /locus_tag="PKY13_14695"
     CDS             2059..2841
                     /locus_tag="PKY13_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT81241.1"
                     /translation="MTPSSSTTPTPTEPSSTGSIQVLQRAALLIRTIGEHRGGLRLAE
                     LGELVDLPKTTIHRIVGALTEERLLRIDPSGRIWLGSSVLALARLAATELAEQLRPVL
                     VELHRVVDETVDLSVLDGSSVHFIDQIQSTQRLRAVSSVGSTFPLHCSANGKAFLASL
                     PLDQLDEMLTRPLERFTEHTITDPDELRAELDVIRAEGVAYDYEEHTLGIAAVAIALT
                     DPTGPIAAISIPTPADRFEINEPRLRAELLAAAAQARTMLSA"
     gene            2902..3066
                     /locus_tag="PKY13_14700"
     CDS             2902..3066
                     /locus_tag="PKY13_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT81242.1"
                     /translation="MQVSDILRHKGTDVITVRPETTIENAVGVLGTRRIGALVVSDDG
                     RTRRRHRLRT"
     gene            3071..3340
                     /locus_tag="PKY13_14705"
     CDS             3071..3340
                     /locus_tag="PKY13_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT81243.1"
                     /translation="MAHLAAVGRTGLDSTVATVMTRDVRTCTPGDALEDVMAIMTAGR
                     FRHLPVVADGELAGIVSIGDAVERRVSELETESARMHDHIQTGRT"
     gene            complement(3395..>3991)
                     /locus_tag="PKY13_14710"
     CDS             complement(3395..>3991)
                     /locus_tag="PKY13_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT81244.1"
                     /translation="LAMTTTRDRLLDEAEALFARTGVAAVTTREIVEAAEQRNTSAVS
                     YHFGSREGLLRAILVRRGGPIDHQRGAMRDELGEQPSLRDLVACLVVPYAAALADPGG
                     RSYLRIVAQLRGRFAVWRVESDATTTKGLARILDEIELRPDASAALRRERVVALIMVM
                     TGTIAERARSIDEGLDPELSHDQFVANLVDMCTALMGA"
BASE COUNT          616 a         1419 c         1369 g          587 t
ORIGIN      
        1 agcgcgatgt gggagccacc catccagaaa cgcgaccgca tctcggcacc gtcggcggtc
       61 gggcggacgt ggtgcacgaa ccagccaccg tcgacgggac gggtggcgtc gccgagccgg
      121 gcgcacacgg tgagctcacc gtcgggcacg tggtcgatgc cgaactccgc cggtggaacg
      181 aaccgaatgg cggcgtggaa gaacgtcgac cccaggtact cccggacgat cgaggtgcgc
      241 ccgacgaagt cgccttcacc agcgccgtcc gcccactccg cggacacatg ggccctcggg
      301 tgccacagct tgtagcggcg gctgtcggag ccgtgccatc cgaaccacca ctcccacatc
      361 gccggcgtca cgccgggcat cggggtgcga accgccacgc ggatcgaccc gtcacgacac
      421 accgcgtacc cgtcctcggt gggttcggac gcggtgcgga cgaactcggc gacgccggcg
      481 cggtcggtga gcaggggctc gggctggggc ccccgggcga gcgcgtcgac gacgtgggga
      541 tggagcggct gcatctccgg ttcgaagaac cggccccacg cagtgtcggc gccgccgtcg
      601 cgatagccca ggtaggtcgt cacgacgcgg gccccgatcc gttcgccccg acgcgaccca
      661 tccacgcgtg gaatcggccg tcggggtcgt aggcggcgcg ggcggcgtcg aggcgctcca
      721 tgttctgcgg gagcacgaac ggcgccggtc gccgccccag gttctcgtcg gccagctgga
      781 tgcccgtcgc caggtcttcc atggacgcca tgttggaccg cgcccagtcg gcgtacaggt
      841 catcgtcggc ggcgtcggtc cacacgccgt agagggcgat gtacacctcg tcctccatcg
      901 agtacgccat gtcctggcgt tcgggcgacg acccccagtt gagccacaga cagtgcgacg
      961 gtgcgggcgg gagcgactcg acgatgcggt gcagtcccgg caacaggtct gcggctgcag
     1021 cggaggtcca catgttgtcg gtggcgtagc ggtggccgtc ggggtagttg tgcatgaccg
     1081 cgtcgtacca ctccatgagg ccggcgggtg cgtacgggat ggcgatcagg gctcggtcga
     1141 tcgccggaca ggtgccgagc agcgccaggg cggcctcggc ctcctcgtcc gagtcggcga
     1201 acacggggga cgcaatgatg atcgccggat cgtggatgtc caactcggcg aactcccggc
     1261 tcatgaggat ctgcatctcg acgcgccgat cgacgtcgtc ggcgatctcc cgggcccacg
     1321 tgaacacctc gtcgagacac tcgatgggat aggcgtagag gctcattccg atcgcgtccg
     1381 gcttgggata cagccggacg tggaatctgg tgaccacgcc gaagaacccc gggccggcac
     1441 cacgtgcgga ccagtacagg tcggcgtggt ggtcggcatc ggcgaacacc tgctcaccgt
     1501 cggcggtgac gacatcgacg ccgatgaccg actggcatgc cggcccgagc acccgcccgt
     1561 tccacccgta cccgccctgc aacaggtagc cgccgacggc gacgcccttg cagtggccgg
     1621 cggggaagaa cagcccctgg tccgcgagcg cggcgcacat ctcgtggccc ttgcggcccg
     1681 gtcccaccgt ggcggtcatg gtggcggggt cgacggcgag cgcgtcgagg cgtgacacgt
     1741 cgaggaggac gccaccgtcg cggacgtggt tggcggccca gctgtgaccg ccggaccgca
     1801 caccgacgcg gtggctgtgc tcggcggcga atcggaccgc ggcgacgacg tcggcctcgg
     1861 tgtgcgcctg gacgatgacg tcggggaagc ggtcgggcag gcgcgcgttc cacacggtcg
     1921 ctcggcgggc agcctcgtag ccgtcgtcgc cccgttcgta cgccaccccg tcggggagtg
     1981 ccaggttcat tcgttcacct ttcgcagcgg atccacatta tggatcccat gatccgttgt
     2041 gtggactata ctgatgccat gactccctcg tcgtcaacca ctcccacgcc caccgagccg
     2101 tcatcgaccg gctcgatcca ggtgctgcag cgcgccgccc tcctgatccg caccatcggc
     2161 gagcaccgcg gcggactccg cctggccgag ctgggcgagc tcgtcgatct ccccaagacc
     2221 acgatccacc gcatcgtcgg cgccctcacc gaggagcggc tgctccgcat cgacccgtcg
     2281 ggtcgcatct ggttgggctc gtcggtgctt gcgctcgcac gcctcgcagc gaccgagctg
     2341 gccgaacaac tccgaccggt cctcgtcgaa ctgcaccgcg tcgtcgacga gacggtcgac
     2401 ctgtccgtgc tcgacggatc gagtgtccac ttcatcgacc agatccagag cacccagcgc
     2461 ctccgtgcgg tgtcgtcggt cggctcgacg ttcccgctgc actgctcggc caacggcaag
     2521 gcgttcctcg catcgctccc cctcgaccaa ctcgacgaga tgctcacgcg ccccctcgag
     2581 cgattcaccg agcacacgat caccgacccc gacgaactgc gtgccgagct cgacgtcatc
     2641 cgggccgaag gcgtcgccta cgactacgag gagcacaccc tcggcatcgc agcggtcgcg
     2701 atcgcgttga ccgatccgac gggacccatc gccgcgatct cgatcccgac gccggccgac
     2761 cggttcgaga tcaacgagcc ccggctccgg gcggagctac tggcggcggc tgcccaggcg
     2821 cggaccatgt tgtccgcgta gcatgtgtga cagacgacac accggacgtc atcggtgtgt
     2881 cgccaccagg aggtaaccgc catgcaggtc tccgacatcc tccgtcacaa gggcacggac
     2941 gtcatcaccg tccgccccga gacgacgatc gagaacgccg tgggggtgct cggcaccaga
     3001 cgcatcggtg cgctcgtggt gtccgacgac ggtcgaaccc gtcgacggca tcgtctccga
     3061 acgtgacgtc gtggcccacc tcgccgccgt cggtcgcacc ggcctcgaca gcacggtcgc
     3121 caccgtcatg acccgtgacg tgcgcacctg cacccccggc gacgccctcg aggacgtcat
     3181 ggcgatcatg acggcgggcc ggttccgcca cctgcccgtc gtcgccgacg gcgagctcgc
     3241 ggggatcgtg agcatcggcg acgccgtgga gcggcgggtc agtgagctcg agaccgagag
     3301 cgctcgcatg cacgaccaca tccagaccgg cagaacgtga cctgatggcc tcgggcctcc
     3361 tccgcttcgc tccggaaccg ctcggccgct tcgctcacgc ccccatcagg gcggtgcaca
     3421 tgtccacgag attggccacg aactggtcgt gggagagttc gggatcgagt ccctcgtcga
     3481 tcgagcgtgc ccgttcggcg atggtgccgg tcatcaccat gatgagcgcc acgacacgtt
     3541 cgcggcgcag cgccgccgat gcgtcgggtc ggagctcgat ctcgtcgagg atccgggcca
     3601 gccccttggt cgtggtggcg tcggactcga cgcgccagac ggcgaagcgg ccccgcagct
     3661 gggcgacgat gcgcaggtac gagcggcccc cggggtcggc gagggccgcg gcgtagggca
     3721 ccacgaggca ggcgacgagg tcgcgcagcg atggttgctc ccccaactcg tcgcgcatcg
     3781 cgccgcgctg gtggtcgatg ggcccacccc ggcggaccag gatcgcccgg aggaggccct
     3841 cccgcgagcc gaagtggtag ctgaccgccg aggtgttgcg ctgctccgcc gcctcgacga
     3901 tctcgcgggt cgtgaccgcg gcgacgccgg ttcgagcgaa cagcgcttcg gcttcgtcca
     3961 gcaggcgatc acgcgtcgtg gtcatggcga g
//
LOCUS       DAOSOA010000311         3969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_22880_length_3969_cov_6.317322, whole genome shotgun sequence.
ACCESSION   DAOSOA010000311 DAOSOA010000000
VERSION     DAOSOA010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22880_length_3969_cov_6.317322"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="hemB"
                     /locus_tag="PKY13_14715"
     CDS             <1..859
                     /gene="hemB"
                     /locus_tag="PKY13_14715"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOT81245.1"
                     /translation="DEPQPISSLPGVVQFDLASLETEVGELVALGVPAVILFGVPLTK
                     DAVGSGASDPDGIVQVALRRLRDRFGDDIVLMADLCLDEYTDHGHCGLLRPDGTVDND
                     STLVRYGEAALAQAAAGVDVVAPSGMMDGQVRAIRAALDGGGHEEIAVLAYAAKYASS
                     LYGPFRDAVDVSIVGGGDRKAYQQDYANVRESLEEIRADIAEGADMIMVKPAVTYLDV
                     LTRARAITDLPLAAYHVSGEYSMIKAAAANGWIDGDAVALEQLTAVKRAGADAILTYF
                     AKEAAAWLS"
     gene            847..2190
                     /locus_tag="PKY13_14720"
     CDS             847..2190
                     /locus_tag="PKY13_14720"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOT81246.1"
                     /translation="MAELEADGRGHQVTNESLYQRGLKVIPGGVNSPVRAFRSVGGTP
                     YFVARGEGAYVWDVEGTRFTDYVQSYGASILGHANETVIAAVRDAALRGTTFGAPTEG
                     EVRLAETLCERVPGMEQVRLVSSGTEAAMSALRLARGVTGRDKVIKFAGHYHGHSDAL
                     LAAGGSGVADQGLSGSAGVTDGAVADTIVAPWNVIPEIGDDVAVVCVEPVAANMGLVP
                     ARDGFLADLRAACDRAGALLLFDEVITGFRVDHGGATALYGVQPDVWCFGKVIGGGLP
                     VGAFGASAEIMSNLAPLGGVYQAGTLSGNPLATAAGLAVLGELTPAAYEQLIGTATTL
                     AEGLAAAFADAGLAAQVPRVGPLIGLFFGPHQPTDYDEAKDSVDLGHYPRWFHGMLDR
                     GIALAPGPYEVLFPSLAHSESDIAHTIEAAAEVAVELAGVAALADAVESENGAAS"
     gene            2187..3734
                     /locus_tag="PKY13_14725"
     CDS             2187..3734
                     /locus_tag="PKY13_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81247.1"
                     /translation="MITARYHLGELEAAVEAAAIELAAADAVTRLWGRDHTLFQEDPA
                     DCANRLGWLDSPTESSDVWDGLVAFAAEIAGESDDVVLMGMGGSSLFPEVLTRTFGSA
                     EGFPNLHVIDSTDPDAVRRVLDATDPARTFHVASSKSGTTLETRSHLDLFWERSGDPD
                     RFAVITDPGSALGELARARGFRHVFENNPDIGGRYAALSLFGMVPAALIDADGDAILE
                     AALDMADALEPLGDGDDADGNIGLRLGAAFGAAARAGRDQLTIVLTPALESFGLWLEQ
                     LVAESTGKHGDGIIPIVGEPIPDVCATPDRRLLVTIGDVEGIAELRASGAPLIELSLE
                     ELHDVGAQVLLWEVAVALAGKVLGINPFDQPDVEAAKAAARELLDADAPPAPPVTPLA
                     DALAAIGPGDLLAVCAFVDPGSDVADDLEEFRSSTARRLGVASTLGFGPRFLHSTGQL
                     HKGGPASIVVVQVCSDPADGHDLEIPGQTFTFGAFKAAQAAGDLAALHASGHRAFRVS
                     LEEISRG"
     gene            complement(3721..>3969)
                     /locus_tag="PKY13_14730"
     CDS             complement(3721..>3969)
                     /locus_tag="PKY13_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81248.1"
                     /translation="ISGDQIDVSFTIAATDVDEFASGSGIELTSGERTITHASPLWDV
                     AVSGEVQGGSSTRNQIGRNAEVLVEGDTATVRLSINRG"
BASE COUNT          537 a         1396 c         1403 g          633 t
ORIGIN      
        1 cgacgagccc cagccgatct cgtcgttgcc cggggtcgtc cagttcgatc tcgcgtccct
       61 cgagaccgag gtgggtgagc tggtggcgtt gggtgtgccg gcggtgatcc tgttcggggt
      121 gccgttgacg aaggatgcgg tggggtcggg agcatcggat cccgacggca tcgtgcaggt
      181 cgcgctgcgg cggctccgtg accggttcgg cgacgacatc gttctcatgg ccgacctgtg
      241 cctcgacgag tacaccgatc acggccactg cggcctgctc cgtcccgacg gcacggtcga
      301 caacgattcc acgctcgtcc gctacggcga ggcggcgttg gcgcaggcgg cggcgggggt
      361 cgacgtcgtg gcaccttccg gcatgatgga cggccaggtg cgggccatcc gtgccgcgct
      421 cgacggtggt gggcacgagg agatcgcggt gctggcctat gccgccaagt acgcgtcgag
      481 cctgtacggg cccttccgcg acgcggtcga cgtatcgatc gtcgggggcg gcgaccgcaa
      541 ggcctaccaa caggactacg ccaacgtccg cgaatcgctc gaggagatcc gggccgacat
      601 cgccgagggc gccgacatga tcatggtcaa gccggccgtc acctacctcg acgtcctgac
      661 ccgggcccgg gcgatcaccg atctgccgct cgcggcgtac cacgtgtcgg gcgagtactc
      721 catgatcaag gcggccgctg cgaacggctg gatcgacggc gacgccgtcg ccctcgagca
      781 gctcacggcg gtcaagcgcg ccggcgccga cgccattctc acctacttcg ccaaggaggc
      841 tgccgcgtgg ctgagctaga agccgatggc cgaggccatc aggtcaccaa cgagtccctc
      901 taccaacggg gcctgaaggt catccccggc ggcgtgaact cgccggtccg tgcgttccgt
      961 tccgtcggtg gcacgcccta cttcgtggcc cgtggcgagg gcgcctacgt gtgggacgtc
     1021 gaaggcacgc gcttcaccga ctacgtccag agctacggcg cgtcgatcct cggccacgcg
     1081 aacgagacgg tgatcgccgc cgtacgcgac gcggcgctgc gcgggaccac gttcggcgca
     1141 ccgaccgagg gcgaggtgcg cctggccgag accctgtgcg aacgcgtgcc gggcatggag
     1201 caggtgcgcc tcgtgtcgtc gggcacggag gcggcgatgt cggcgctccg tctggcccgc
     1261 ggcgtcaccg gccgcgacaa ggtcatcaag ttcgcgggcc actaccacgg tcattccgac
     1321 gcgctgttgg cagccggcgg atcgggcgtc gccgaccagg gcctctccgg gtcggcaggc
     1381 gtgaccgacg gcgcggtcgc cgacacgatc gtcgccccgt ggaacgtgat ccccgagatc
     1441 ggcgacgacg tcgcggtggt gtgcgtcgaa ccggtggcag ccaacatggg actggtgccg
     1501 gcgcgcgacg ggttcctcgc cgatcttcgt gcagcgtgcg atcgagccgg cgcactcctc
     1561 ctgttcgacg aggtgatcac ggggttccgc gtcgaccatg gcggagcgac ggcgctctac
     1621 ggtgtccagc ccgacgtctg gtgcttcggg aaggtcatcg gtggtggtct gcccgttgga
     1681 gcgttcggcg cgtcggcgga gatcatgtcc aacctcgcgc cgctcggcgg cgtctaccag
     1741 gccggcacct tgtcggggaa cccgctggcg acggctgcgg gcctcgccgt gttgggcgag
     1801 ctgacgcccg ctgcgtacga gcagctgatc ggcacggcca cgaccctcgc cgagggcctc
     1861 gcggcggcgt tcgccgatgc cggtctcgcc gcccaggtgc cccgggtcgg gccgctcatc
     1921 ggactcttct tcgggccgca ccagccgacg gactacgacg aggcgaagga ctccgttgat
     1981 ctcgggcact acccgcgctg gttccacggc atgctcgacc gcggcatcgc gctggcgccc
     2041 ggcccctacg aggtgctgtt cccgtcgctg gcgcacagcg agtcggacat cgcccacacg
     2101 atcgaggcgg ccgccgaggt cgccgtcgag ctggccgggg tggcggcact cgccgacgcc
     2161 gtcgagtcgg agaacggagc tgcgtcgtga tcaccgcccg ctatcacctc ggtgagctcg
     2221 aggccgcggt cgaggccgct gccatcgagc tggccgccgc cgatgccgtg acccgcctgt
     2281 ggggtcggga tcacaccctg ttccaggagg atccggccga ctgcgccaac cggctgggct
     2341 ggctggactc gccgaccgag agctccgatg tgtgggacgg cctcgttgcg ttcgccgcgg
     2401 agatcgcggg ggagagcgac gacgtggtgt tgatgggcat gggcggctcg tcgttgttcc
     2461 ccgaggtgct cacgcgcacc ttcgggtcgg cggagggctt cccgaacctg cacgtgatcg
     2521 acagcaccga tcccgacgcc gtccggcggg tgctggatgc aaccgatcct gcgcgcacgt
     2581 tccatgtcgc gtcgtcgaag tcgggcacga cgctcgagac ccgttcgcac ctcgatctgt
     2641 tctgggagcg ctccggcgac cccgaccggt tcgccgtcat caccgacccc gggtcggcgc
     2701 tcggcgaact cgcccgtgca cgcgggttcc gtcacgtctt cgagaacaac cccgatatcg
     2761 gtggccggta cgcggcgctc tcgctgttcg gcatggtgcc ggcagcgctc atcgatgccg
     2821 acggcgacgc gatcctcgag gccgcgctcg acatggctga cgccctcgag ccactcggcg
     2881 acggtgacga cgccgacggc aacatcggtc tgcgcctcgg cgcagcgttc ggcgccgcgg
     2941 cacgtgctgg gcgcgaccaa ctcacgatcg ttctcacccc ggcgctggag tcgttcgggc
     3001 tgtggctcga gcagctcgtt gccgaatcca ccggcaagca cggcgacggc atcatcccga
     3061 tcgtcggcga accgatcccc gacgtgtgcg ccactcccga ccgtcggttg ctcgtcacca
     3121 tcggcgacgt cgagggcatc gccgagctac gggccagtgg cgccccgctc atcgaactgt
     3181 ccctcgaaga gctgcacgac gtgggagcgc aggtgctgct ctgggaggtc gccgtcgcgc
     3241 tggccggcaa ggtcctgggc atcaacccgt tcgaccagcc cgacgtcgag gcggcgaagg
     3301 cagcggcgcg ggagctgctc gacgcggatg ccccgccggc cccgccggtg acgccgctcg
     3361 ccgacgcact cgctgcgatc ggtcccggtg atctcctcgc cgtgtgcgcg ttcgtcgacc
     3421 cgggctccga cgtcgccgac gacctcgagg agttccgctc atcgacggcc cggcgcctcg
     3481 gtgtcgcctc caccctcggg ttcggtcccc ggttcctcca ctccaccggg cagctgcaca
     3541 agggtgggcc ggcgagcatc gtggtcgtgc aggtgtgctc cgaccctgcc gatggtcacg
     3601 acctcgagat tcccggtcag acgttcacgt tcggcgcctt caaggcggcg caggcggccg
     3661 gcgacctcgc ggcgctgcac gcgtcgggcc accgggcctt ccgtgtgtcg ctcgaggaga
     3721 tcagccgcgg ttgatcgaca ggcgcaccgt cgcggtgtcg ccctcgacca gcacttctgc
     3781 gttccgaccg atctggttgc gggtcgacga cccgccctgc acctcgccgc tgactgcgac
     3841 gtcccagagg ggtgaggcgt gggtgatcgt tcgctcgccg gacgtcagct cgatgcccga
     3901 gccggatgcg aactcgtcga cgtcggtcgc cgcgatggtg aacgacacgt cgatctggtc
     3961 accgctgat
//
LOCUS       DAOSOA010000312         3945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_23053_length_3945_cov_5.661697, whole genome shotgun sequence.
ACCESSION   DAOSOA010000312 DAOSOA010000000
VERSION     DAOSOA010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23053_length_3945_cov_5.661697"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKY13_14735"
     CDS             complement(<1..407)
                     /locus_tag="PKY13_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81249.1"
                     /translation="MTTRTALGALATVLLLTPLAGCGIVGGGNSVVLVGDSLTVLVSD
                     QVAADAKADYDIEVTATWGVRIDEELDPAAIAAESSPDQVIINIGTNNVLQRHDTTAS
                     AEDLAAMLDLFDDASCLHLVTVNERINRLGDDFG"
     gene            complement(427..702)
                     /locus_tag="PKY13_14740"
     CDS             complement(427..702)
                     /locus_tag="PKY13_14740"
                     /inference="COORDINATES: protein motif:HMM:NF022683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HOT81250.1"
                     /translation="MSDSPSVQILPDIGYDLDLESVLGETPPEECAHIVRRSGALGAG
                     ALIVAAAESGMEVEALCGYRWVPKGHAPDDLPACVRCVEVWQSLTSQ"
     gene            750..823
                     /locus_tag="PKY13_14745"
     tRNA            750..823
                     /locus_tag="PKY13_14745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Ile,seq:cat)
     gene            complement(879..>1727)
                     /locus_tag="PKY13_14750"
     CDS             complement(879..>1727)
                     /locus_tag="PKY13_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOT81251.1"
                     /translation="NFMRNAFIGGTLVALASGLIGYFVVIRRDAFAAHALAHIGFPGA
                     TGAVLIGAPVTLGLAVFCIAGGLAIGALGKRVDRREVATGTILAFATSLGVLFASLAT
                     RSTNTVTSVLFGNLLAISSDQLVTFAVFTVVLLIAVASIARPLLFASITPQVAEAKGL
                     PVRALGIAFMVMLALVVTMAVQVVGTLLLFALVVSPAATAIAITARPSAVVAIGTAIG
                     LGAVWAGLVASAMFNLPPSFPIVAIVFVGWLITLGLTRDRGHGAASGDIVHDHHHPPE
                     TTNLSI"
     assembly_gap    1728..1827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1828..2378)
                     /locus_tag="PKY13_14755"
     CDS             complement(<1828..2378)
                     /locus_tag="PKY13_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT81252.1"
                     /translation="MTAAASLDGVSIVRQGRTIWSDGTFTVPTGSVVGVIGPNGSGKT
                     TLLQMLLGLLTPTTGDIEVLGSTPSRGNPRIGYVPQNYTAAIGEAVRCRDLVELGLTG
                     SRWGLRRGGDETRQLVDEALRSVGAEAFARRRMSTVSGGQQQRVAIAQGLVHEPELLL
                     LDEPLANLDLRSQHDIVALLGSLA"
     gene            complement(2375..3295)
                     /locus_tag="PKY13_14760"
     CDS             complement(2375..3295)
                     /locus_tag="PKY13_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOT81253.1"
                     /translation="MKLTRLMLPVLVLAALGTGCGSDSDTATGDSSSTAGSCPTEPVS
                     VVVTVDQWGDIVEQLGGDCAEVTTVIKGSSADPHDYEPTPADTAKFDDAQLVVVNGLD
                     YDHWAEKAVDNLDSEPAVVDGGKVVGLEEGDNPHIWYGPDYVRQISTAVTSELKELAP
                     DAAAYFDEQQTAWQTSMKPYDDVITRITAVAADKTFAASEPVFDYMADALGLTNGTPK
                     GYQDAALNESDPSPADIADFGQSLRDEEIDVLVYNTQTEGPTPEQIRGVADDADVAVV
                     DVTETVPPGTTSFVTWQVDQLTALATALGA"
     gene            complement(3402..3863)
                     /locus_tag="PKY13_14765"
     CDS             complement(3402..3863)
                     /locus_tag="PKY13_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOT81254.1"
                     /translation="MARHASTEPPSDLHATAASRLRALGHTYPTRRRELVDILAAAPQ
                     PVSIPDVLDQDRGLAQSSVYRNLAILELAGIVHRIVTKDEFARFELSEELSGHHHHHL
                     ICSSCGAVEDVTLPASVETSVTRELNKLAASRGFAASSHQMDLVGLCAVCQ"
BASE COUNT          595 a         1224 c         1419 g          607 t
ORIGIN      
        1 ccgaagtcgt cgcccaggcg gttgatgcgc tcgttgacgg tcacgaggtg caggcagctc
       61 gcgtcgtcga agaggtcgag catggcggca aggtcctccg ccgacgcggt ggtgtcgtgg
      121 cgttggagca cgttgttcgt gccgatgttg atgatcacct ggtcgggcga cgattcggcg
      181 gcgatcgccg cgggatcgag ctcctcgtcg atccgtacgc cccacgtggc ggtgacctcg
      241 atgtcgtagt cggccttcgc gtcggcggcg acctggtcgc tgacgagcac cgtgagcgag
      301 tcgcccacga ggacgacgct gttgccaccg cccacgattc cgcacccggc gaggggagtg
      361 agcaggagga cggtcgccag tgcacccagc gcggttcgcg tcgtcatcgg tggtcgatgc
      421 tgagcgctac tgggaggtga gcgattgcca gacctcgacg caccggacgc acgccggcag
      481 gtcgtcgggg gcgtggccct tgggcaccca gcggtacccg cacagggctt cgacctccat
      541 gccggactcg gctgccgcga cgatcagggc gccggcgccg agggcgcccg agcggcgaac
      601 gatgtgtgcg cattcctcgg gcggcgtctc gccgagcacc gactcgaggt cgaggtcgta
      661 cccgatatcg gggagtatct gcaccgaggg cgagtcgctc acggcagcga ggttagcaac
      721 ggtggagcgc cagtaggatg ccggccttcg gggctgtagc tcagctggtc agagcagggg
      781 actcataatc ccttggtcgc gggttcgagc cccgccggcc ccacccgacg cggtgtgtgc
      841 tcgcgagacc gtgatgtggc gcacgccgag ggcgccgctc agatgctcag gttggtcgtc
      901 tcgggcggat gatggtggtc gtggacgatg tcgccggagg cggcgccatg cccgcggtcc
      961 cgggtgagtc cgagggtgat gagccagccg acgaacacga tcgcgacgat cgggaacgac
     1021 ggtggaaggt tgaacatggc cgaggcgacc aggcccgccc acaccgcacc gagtccgatc
     1081 gccgtgccga tcgccacgac agcgctcggt cgcgcggtga tcgcgatggc ggtggcggcg
     1141 ggggagacga cgagcgcgaa caggaggagc gtcccgacga cctgcacggc catggtgacg
     1201 acgagtgcca gcatcaccat gaacgcgatg ccgagcgccc ggacgggaag ccccttcgcc
     1261 tcggcgacct ggggcgtgat cgaggcgaag agcagcggcc gggcgatcga cgcgacggcg
     1321 atcaggagca ccaccgtgaa gaccgcgaac gtcacgagtt ggtccgacga gatcgcgagc
     1381 aggttgccga acagcacgct ggtgacggtg ttggtgctac gggtcgcgag cgaggcgaac
     1441 agcacgccga gcgacgtggc aaacgccaag atggtgccgg tcgccacttc gcgacggtcg
     1501 acgcgcttgc cgagcgctcc gatggcgagc ccgccggcga tgcagaacac ggcgagcccg
     1561 agggtgacgg gcgctccgat caggacggcg ccggtggcgc ccgggaatcc gatgtgggcg
     1621 agcgcgtggg cggcgaaggc gtcgcggcgg atgaccacga agtacccgat gaggcccgac
     1681 gccaaggcga ccagggtgcc gccgatgaac gcgttgcgca tgaagttnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg agcgagccga gcagcgcgac gatgtcgtgc
     1861 tggctccgca ggtcgaggtt ggcgagcggt tcgtcgagga ggaggagctc cggttcgtgc
     1921 acgagacctt gtgcgatggc gacccgctgc tgctgcccgc ccgacacggt ggacatgcgg
     1981 cggcgtgcga acgcctcggc acccaccgat ctcagtgcct cgtcgacgag ctggcgggtc
     2041 tcgtcgccgc cccgtcggag cccccagcgg gagccggtga ggccgagctc gacgaggtcg
     2101 cggcaccgca cggcctcgcc gatggcagcg gtgtagttct gcggcacgta gccgatgcga
     2161 gggttcccgc ggctcggcgt cgaacccagg acctcgatgt cgccggtcgt cggcgtgagg
     2221 agcccgagca gcatctgcag gagcgtggtc ttgcccgacc cgttcgggcc gatcaccccg
     2281 acgaccgacc cggtcggcac ggtgaaggtt ccgtcggacc agatggtgcg accctgccgg
     2341 acgatggaaa cgccgtcgag agatgcggct gcggtcacgc ccccagggcg gttgccagcg
     2401 ccgtcagttg gtccacctgc cacgtcacga agctagtggt ccccggaggc accgtttccg
     2461 tgacgtcgac caccgcgacg tcggcgtcgt cggcgacgcc acgaatctgc tcgggcgtgg
     2521 ggccctcggt ctgggtgttg tagacgagca cgtcgatctc ctcgtcccgc aacgactgcc
     2581 cgaagtcggc gatgtcggcg ggtgacgggt cggactcgtt gagcgcagcg tcctggtagc
     2641 ccttcggtgt gccgttggtg aggccgagcg cgtcggccat gtagtcgaag accggctcgc
     2701 tcgccgcgaa cgtcttgtcg gctgccacgg cggtgatccg ggtgatcacg tcgtcgtacg
     2761 gtttcatcga ggtctgccac gcggtctgct gctcgtcgaa gtaggcggcg gcgtccggcg
     2821 ccagttcctt cagctcggac gtgaccgccg tcgagatctg gcggacgtag tcgggtccgt
     2881 accagatgtg ggggttgtcg ccttcctcca gcccgacgac ctttccgccg tcgacgaccg
     2941 ccggttcgga atcgaggttg tcgaccgcct tctcggccca gtggtcgtag tccaggccgt
     3001 tgacgacgac gagctgcgcg tcgtcgaact tggcggtgtc ggccggcgtc ggctcgtagt
     3061 cgtgagggtc ggccgacgaa cccttgatca cggtcgtcac ctcggcgcag tcgcccccca
     3121 gttgttcgac gatgtcgccc cactggtcga cggtcaccac gaccgagacc ggctcggtcg
     3181 ggcacgagcc ggccgtggac gagctgtcac cggttgccgt gtcggagtcg gagccgcagc
     3241 cggtgcccag cgctgcgagc accaggacgg ggagcatgag tcgtgtgagc ttcatcgggt
     3301 tcgacctcca agtatgggta tgagacgcat tcccattccc atagagtcaa gcccgctccg
     3361 ctgggagccg tcaagcgccg acgtcggtgg agatcacccc gttactggca gacggcgcag
     3421 aggccgacga gatccatctg gtggctcgac gctgcgaacc cccgggatgc cgcgagcttg
     3481 ttcagctctc gggtcacact cgtctcgacc gatgccggga gggtgacgtc ctcgacggcc
     3541 ccgcacgacg agcagatgag gtgatggtga tggtgaccgc tcagctcctc ggacagctcg
     3601 aagcgggcga attcgtcctt ggtcacgatg cgatggacga tgccggccag ttcgagaatg
     3661 gcgaggttgc ggtacaccga gctctgggcg agtcctcggt cctgatccaa cacgtcgggg
     3721 atcgacaccg gctgcggcgc ggccgccagg atgtcgacga gttctcgtcg gcgcgtgggg
     3781 taggtgtggc ccagcgctcg cagacgagac gccgcggtgg cgtggaggtc ggatgggggt
     3841 tcggtcgatg cgtgtcgggc catggcggat cggttccctc acgttggccg cggaccgcgg
     3901 cggaactgac ccaccctacc aatccgtgag aaccgttcct ggccc
//
LOCUS       DAOSOA010000313         3894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_23444_length_3894_cov_6.634540, whole genome shotgun sequence.
ACCESSION   DAOSOA010000313 DAOSOA010000000
VERSION     DAOSOA010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23444_length_3894_cov_6.634540"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PKY13_14770"
     CDS             complement(<1..152)
                     /locus_tag="PKY13_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81255.1"
                     /translation="MTDITRARYAQLFGPTAGDRIRLADTNLLIEVEHDHCGGSSGGA
                     GEEVLFG"
     gene            complement(149..487)
                     /locus_tag="PKY13_14775"
     CDS             complement(149..487)
                     /locus_tag="PKY13_14775"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HOT81256.1"
                     /translation="MLVIPGEVFVGDEPVALFGDAELITLEVVNAGDRPIQVGSHYHF
                     AEANPSLTFDREQARGYRLAIAAGTAVRFEPGIDRTVELVPIAGARIVRGLRGDVAGP
                     LEAPVNGGAS"
     gene            complement(492..824)
                     /locus_tag="PKY13_14780"
     CDS             complement(492..824)
                     /locus_tag="PKY13_14780"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HOT81257.1"
                     /translation="MVDAPTGSIALQISLHEQERLMISLAAMVARDRQARGLKLNCPE
                     AVALLTSFVLEGARDGRRVADLMEAGRAVLGRDDVMEGVPEMIDEVQVEATFPDGTKL
                     VTIHQPIP"
     gene            complement(905..1255)
                     /locus_tag="PKY13_14785"
     CDS             complement(905..1255)
                     /locus_tag="PKY13_14785"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOT81258.1"
                     /translation="MKRLAILWAISTLALLLLPVLFSDVEVDDWKYAALASVVIGLIN
                     CSIGPILKLFTLPIRWLTLGFFTLIINALLLLLASEVAPGFVINGFWTAFWAAIVYSI
                     VTWIGGLLLVRDDD"
     gene            complement(1252..>1584)
                     /locus_tag="PKY13_14790"
     CDS             complement(1252..>1584)
                     /locus_tag="PKY13_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81259.1"
                     /translation="VPGELIGFRVPFMPLAHVFRAGSRIRVGVSTPGRDMPLWCYDNP
                     VTEGAVHEVGVGGTAASSLVLPVWDDPAHLLAPEHEAHPPADALRGQPARRARPVANT
                     RAGLRFDL"
     assembly_gap    1585..1684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1685..3196)
                     /locus_tag="PKY13_14795"
     CDS             complement(<1685..3196)
                     /locus_tag="PKY13_14795"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOT81260.1"
                     /translation="MHDGSSTLRLQPGVETLTITGAEPGTALRVDRGDTPLVTVVADG
                     AGNAHVAFVPTEHGRIETAEQWAEVLSTGELLAPGPYTVVDVATGERHGPVTVLAVGD
                     VPDESLYDQELAEGFGYLEVRDGVTLSIMVRFPNEDLYGPAPWPTVIEYSGYGPSNPD
                     APQPGTLLANLLGFAVVGVNMRGTGCSGGVFDVFSPAQAADGYDVVETVARQPWVLDR
                     RVGMVGLSYPGISQLYVAATQPPSLAAITPLSVIDDLWRQQWPGGTYNSGFTRSWLAA
                     RDAETKVGGQAWDQARIDAGDAQAAANQAIRSQNFDFERFGRAIDNFRPTLEARRVES
                     VLDRIDVPVYLTGAWQDEQTGSRFALMLDRFHNAPHVQFTLFNGHHPDGYSPMVILRW
                     FEFLSFHVGRRVPVVPELIRMFAPMQFAEVFGVVQELEADRYGDLVDDFDAAFARFTS
                     EPPVRIMFESGAGTDTPGATGARYEVLTTEFPPPGTVARRWWFGSDGTLDDEAS"
     gene            3260..>3894
                     /locus_tag="PKY13_14800"
     CDS             3260..>3894
                     /locus_tag="PKY13_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT81261.1"
                     /translation="MDAFFNHAGASPPTDAVVERMIRHLRREQEIGGYEAAAEVVDEL
                     AGVRATIADAIGAQPVEVALTAGATDAWERLAWAYLSQQRFDGGATIVVDPFTYVSMW
                     TTLQRWNVLRPLTIVVAEPAPDGTVDLDSLSSLVEPDTAMVLLTHIPTHCGTVTPAAA
                     AIAAARASAPEVLIALDIAQSAGQMALDVQALGSDVAFAPGRKFLRGPRGTG"
BASE COUNT          628 a         1321 c         1307 g          538 t
ORIGIN      
        1 ccgaacagca cttcctcacc ggcgcccccc gaggagccgc cacagtggtc gtgctcgacc
       61 tcgatgagca ggttcgtatc ggcgagccgg atgcgatcac cagccgtcgg gccgaacaac
      121 tgggcgtacc gggctcgggt gatgtcggtc atgacgcacc gccgttgacc ggtgcttcga
      181 ggggcccggc gacgtcgccg cgcaggccgc gaacgatgcg ggctccagca atcggcacca
      241 gctcgacggt gcggtcgatg cccggttcga accggaccgc ggtgcccgcg gcgatggcga
      301 ggcggtaccc gcgtgcctgt tcgcgatcga aggtcaacga cggattggcc tcggcgaagt
      361 ggtagtggga tccgacctgg atgggccggt cgccggcgtt gacgacctcg agggtgatga
      421 gctcggcgtc gccgaacagc gccacgggct cgtcaccgac gaacacctca ccggggatca
      481 cgagcacccg ctcacgggat cggctggtgg atcgtcacca gcttcgtgcc gtcggggaag
      541 gtcgcctcga cctgcacctc gtcgatcatc tcgggcacac cctccatcac gtcgtcacga
      601 ccgagcacgg cgcgtccggc ttccatgagg tcggcgaccc gccggccgtc gcgagcgcct
      661 tcgagcacga aggaggtcag caacgccacg gcctcgggac aattgagttt gagccctctg
      721 gcctgccggt ccctcgccac catcgcggcg agggagatca tcaggcgctc ttgttcatgg
      781 agcgagatct gcaaggcaat cgatcctgtc ggtgcgtcga ccatgggagg gcggtcgagg
      841 agaagctacg aaggggccgt gtccccgggg ttgcccgttt gttgccgacg tgtgaacgcc
      901 gcgctcagtc gtcgtcgcgg acgaggagca gcccgccgat ccaggtcacg atgctgtaga
      961 cgatggccgc ccagaacgcc gtccagaacc cgttgatcac gaagccggga gcaacctcgg
     1021 aggcgaggag caacagcaag gcgttgatga tcagggtgaa gaacccgagg gtgagccagc
     1081 ggatcggcag cgtgaacagc ttcaggatcg gcccgatcga acagttgatg agaccgatga
     1141 cgaccgaggc caacgccgca tacttccagt cgtccacctc gacgtcgctg aacaacaccg
     1201 ggaggagcag cagggccaac gtcgagatgg cccacaggat cgcgaggcgc ttcacaggtc
     1261 gaaccgtaga ccagctcggg tgttggcgac ggggcgggcg cggcgggcgg gttggccacg
     1321 gagggcgtcc gccggtgggt gggcctcgtg ttcgggagcg aggaggtggg ccgggtcgtc
     1381 ccacaccggc aacacgagcg acgaagccgc cgtgccgccg acccccacct cgtggaccgc
     1441 accctcggtc acggggttgt cgtagcacca caacggcatg tcccgcccgg gcgtggagac
     1501 acccacccgg atccgcgagc cggcccggaa cacgtgcgcc aacggcatga acggcacccg
     1561 gaacccgatc agttcgcccg gcacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnncgacgc ttcgtcgtcg agggtcccgt cggagccgaa ccaccagcgg cgcgccaccg
     1741 ttcccggcgg tgggaactcg gtcgtcaaca cctcgtagcg agcccctgta gcgcccggcg
     1801 tgtcggtgcc ggcgccgctc tcgaacatga tgcgcaccgg cggttcggag gtgaaccgcg
     1861 cgaacgcggc gtcgaagtcg tcgacgagat cgccgtaccg gtccgcctcg agctcttgaa
     1921 ccacaccgaa cacctcggcg aactgcatcg gcgcgaacat gcggatcagc tccggcacca
     1981 cgggcacccg gcgcccgacg tggaacgaga ggaactcgaa ccatcgcagg atcaccatcg
     2041 ggctgtaccc gtcggggtgg tgaccgttga acagcgtgaa ctggacgtgc ggcgcgttgt
     2101 ggaagcggtc gagcatcagc gcgaaccggc tgccggtctg ctcgtcctgc cacgcgccgg
     2161 tcaggtagac gggcacgtcg atccggtcga gcaccgattc gacgcggcgc gcctcgagcg
     2221 tcggacggaa gttgtcgatc gcacggccga accgctcgaa gtcgaagttc tgcgatcgga
     2281 tcgcctggtt ggccgccgcc tgcgcgtcgc cggcgtcgat ccgggcctgg tcccaggcct
     2341 gacccccgac cttcgtctcg gcgtcacgcg ccgccaacca cgagcgggtg aaccccgagt
     2401 tgtacgtgcc tccgggccac tgctgacgcc agagatcgtc gatgaccgac agcggagtga
     2461 tcgcggcgag actcggcggc tgcgtcgcgg ccacgtacag ctgggagatt ccgggatagc
     2521 tgagaccgac catgccgacg cggcggtcga gcacccacgg ctggcgggcg acggtctcga
     2581 ccacgtcgta gccgtcggcg gcctgcgccg gactgaacac gtcgaacacc ccaccggagc
     2641 agccggtgcc gcgcatgttg acgccgacga cggcgaagcc gagcaggttg gcgagcaacg
     2701 tgcccggttg gggcgcatcg gggttggacg ggccgtaccc ggagtattcg atgacggtcg
     2761 gccacggtgc cggcccgtag aggtcttcgt tggggaaccg caccatgatc gacagggtca
     2821 ccccgtcgcg cacctcgagg tacccgaatc cctcggcgag ctcctggtcg tacagcgact
     2881 cgtcgggaac gtccccgacc gcgagcacgg tgaccgggcc atgccgctcc cccgtcgcga
     2941 cgtcgacgac cgtgtacgga ccgggtgcca ggagctcacc cgtcgagagg acctccgccc
     3001 actgctccgc agtctcgatc cgcccatgct ccgtcggcac gaacgcgacg tgcgcgttgc
     3061 cggcaccgtc ggcgacgacg gtcacgagcg gggtgtcgcc acgatcgacg cggagcgctg
     3121 tgcccggctc tgctcccgtg atcgtcaacg tctccacgcc tggttgcagg cgcagcgtgc
     3181 tgctgccgtc gtgcatgtgt ctccccacac tcgaaaaatg gcgcccggcc cgggcgccac
     3241 ccacggatac tgtcgcgccg tggacgcctt cttcaaccac gccggtgcct cgcccccgac
     3301 cgacgcggtc gtcgagcgca tgatccgcca cctgcgccgc gagcaggaga tcggcggcta
     3361 cgaagccgcg gccgaagtgg tggacgaact ggcgggcgtc cgcgccacga tcgccgacgc
     3421 gatcggggcg caacccgtag aggtcgcgct gaccgccggg gcgaccgatg catgggaacg
     3481 cctggcctgg gcgtacctgt cgcagcagcg cttcgacggc ggcgcgacga tcgtcgtcga
     3541 ccccttcacc tacgtctcga tgtggacgac gctgcaacgt tggaacgtcc tgcgtccgct
     3601 cacgatcgtc gtggccgagc cggctcccga cggcacggtc gacctcgact cgctgtcctc
     3661 gctcgtcgaa cccgacaccg cgatggtgct gctcacccac atcccgacgc actgcggcac
     3721 ggtcaccccg gcggccgccg cgatcgccgc ggcgcgagcg tcggcacccg aggtcctgat
     3781 cgcgctcgac atcgcccagt ccgccggcca gatggcgctc gacgtgcagg cgctcggcag
     3841 cgacgtggcg ttcgcaccgg gccgcaagtt cctccgagga ccgaggggaa cagg
//
LOCUS       DAOSOA010000314         3894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_23445_length_3894_cov_6.308414, whole genome shotgun sequence.
ACCESSION   DAOSOA010000314 DAOSOA010000000
VERSION     DAOSOA010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23445_length_3894_cov_6.308414"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKY13_14805"
     CDS             complement(<1..242)
                     /locus_tag="PKY13_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81262.1"
                     /translation="MIPLSTSTLGRATPPPRRRGPLIVVALATVIVVPFVIRDLVTGD
                     DEKVTSSGTTTTVAAGAGAPTTTAVTTTTGAPKDGD"
     gene            complement(239..997)
                     /locus_tag="PKY13_14810"
     CDS             complement(239..997)
                     /locus_tag="PKY13_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT81263.1"
                     /translation="MWIDNHCHLPAGDSALAADLVAEAAAGDVTRLINVGCDVAESRA
                     AIEVARAHDGVWATAGVHPHDAAQGIEGLRELLTDPSVVAVGECGLDYHYNKSPAAEQ
                     RAVFAQQIRLAHEFDLALVIHTREAWDETFEILAAETVPDRTVFHCFTGGPVEAERCL
                     DLGAVLSFSGIVTFPSAPELREAALLCPLDRMMVETDAPFLAPVPHRGKPNRPALVSV
                     VGAAIAELREIDTADLATATWTTAERFYRLPATA"
     gene            1063..2124
                     /locus_tag="PKY13_14815"
     CDS             1063..2124
                     /locus_tag="PKY13_14815"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOT81264.1"
                     /translation="MSVPDDDERLRRRPKVLLHDHFDGGIRTATIIDLANDIGYRDLP
                     TYDVDDLAAWFHRGADRRSLELYLETFVHTVAVLTTPEAIERVAAECAEDLAADGVVY
                     AESRLAPEEVVHADPTGRLTMDDAIIAMRRGFDSRADLGIEMRTIVCAMRNHSLSADA
                     ARAAVRNRDHGVVGFDIAGPETGFPPSEHLEAFDIARAGGLGITIHAGEAAGIGSIRE
                     ALVDCGADRLGHGVRIVDEVGFDGDGAVALGPVAQMVRDSGTCLELCPTSNVHTAAAG
                     VMSLADHPIALLADCGFAVTINTDNRLMSDVTVLDEYRRLAETFGWGDAEFNAANRTA
                     LRTAFCDDATRARLAPRFD"
     gene            complement(2148..2987)
                     /locus_tag="PKY13_14820"
     CDS             complement(2148..2987)
                     /locus_tag="PKY13_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT81265.1"
                     /translation="MTELGAPKTYKLYSGGAFIRSESGRVYPVAGVDGGTVRVAQASR
                     KDLRDAVVKARAAQSGWGAATPYLRGQILYRIAEMLDGRRDQFVAELENGGVDTYDAA
                     VEVDDAVDTWVYWAGWTDKLTQVLGGVNPVAGPFLNVSTPEPTGVVGVIAPTERPLSG
                     LSALLAPVLCGGNTAVVLASEASPFAPLTFGEVLATSDVPAGVANLLVGPVAELAPWL
                     ADHHDVDALDISGVVDTELAIDLTVRSADNLKRVARTDGVRSPRTAGEFLEIKTVWHP
                     ARL"
     gene            complement(2984..>3894)
                     /locus_tag="PKY13_14825"
     CDS             complement(2984..>3894)
                     /locus_tag="PKY13_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT81266.1"
                     /translation="TSLTALLLAEVIAEADLPPGVVNIVTGGGATGAALSSHRGVDKV
                     AFTGSTEVGRAIRRATAATGARVTLELGGKSAHVIFDDAPIDQAVEGVVRGIWFNQGH
                     VCCAGSRLLLQESIADEFLEKLRRRMELLRIGDPLDKNTDIGAINSREQLDRIVELVA
                     AGVDEGAEIVQSSCPLPETGWFYPPTILSGVDTSHRVATEEIFGPVLAVMTFRTPEEA
                     VAKANATPYGLAAGVWTESGSRILWMSRKLRAGVVWANTYNQFDPTSPFGGMRESGFG
                     REGGRHGLEAYLDLSPLGVDRIGVGA"
BASE COUNT          594 a         1392 c         1347 g          561 t
ORIGIN      
        1 tcgtcgccgt ccttcggtgc tcccgtggtg gtggtcacag cagtggtggt cggagcgccg
       61 gcgccggcgg ccaccgtcgt ggtggtgccc gacgaggtca ccttctcgtc gtcacccgtg
      121 acgaggtcgc ggatgacgaa cggcaccacg atcaccgtcg cgagggcgac cacgatcagc
      181 gggccgcgtc gccgcggtgg gggcgtggca cggcccaggg tggatgtcga cagcgggatc
      241 atgcagtggc aggcagtcgg tagaaccgct ccgcggtcgt ccacgtcgcc gtggcgaggt
      301 cggccgtgtc gatctcccgc agctcggcga tcgctgcgcc caccacgctg accagcgccg
      361 gccggttcgg cttgccccga tggggcaccg gagcgaggaa gggtgcgtcg gtctcgacca
      421 tcatgcggtc gagcgggcac agcagcgccg cctcgcgcag ctcgggagcc gacgggaacg
      481 tgacgatgcc gctgaaggag agcaccgccc cgagatccag gcaacgctcc gcctcgaccg
      541 gaccgccggt gaagcagtgg aacacagtcc gatcgggcac cgtctcggca gcgaggatct
      601 cgaaggtctc gtcccacgcc tcgcgggtgt ggatgacgag cgccaggtcg aactcgtgcg
      661 ccagacggat ctgctgggcg aacaccgctc gctgttcggc ggccggcgac ttgttgtagt
      721 gataatcgag tccgcactca ccaacggcga cgacgctggg atcggtgagg agttcccgca
      781 ggccctcgat gccctgcgcc gcatcgtggg gatggacgcc ggcggtcgcc cacacgccgt
      841 catgggctcg ggcgacctcg atcgcggcgc gggactcggc cacgtcgcag ccgacgttga
      901 tgaggcgcgt gacatcgccg gcggccgcct cggccacgag gtctgccgcc agcgcgctgt
      961 cgccggcggg cagatggcag tgattgtcga tccacatcgc cgacagcctc gctcagatcc
     1021 cgtcccgcaa gccagccatc cccggtgcgt agagtgaccg tcgtgtccgt tccagacgac
     1081 gacgagcgcc tgcgacggcg gccgaaggtg ctcctccacg accacttcga cgggggcatc
     1141 cgaacggcga cgatcatcga tctcgccaac gacatcggct accgcgacct ccccacctac
     1201 gacgtcgacg acctcgccgc gtggttccac cggggcgccg accgtcgcag tctcgagctg
     1261 tacctcgaga ccttcgtgca caccgtggcg gtgctcacca cccccgaggc catcgagcgg
     1321 gtcgccgccg agtgtgccga ggacctcgcc gccgacggcg tggtgtacgc cgagtcacgg
     1381 ctcgctcccg aggaggtcgt gcacgccgac cccaccggcc ggttgaccat ggacgatgcg
     1441 atcatcgcaa tgcggcgggg tttcgactcc cgggccgacc tcggcatcga gatgcggacg
     1501 atcgtgtgcg ccatgcgcaa ccactcactg agcgcagacg cggcgcgggc cgccgtgcgg
     1561 aaccgggacc acggcgtcgt cggcttcgac attgccgggc ccgagaccgg cttccctccg
     1621 tccgagcacc tcgaggcctt cgacatcgcc cgcgccggtg ggttgggcat caccatccac
     1681 gccggcgagg ccgccggcat cggttcgatc cgcgaagccc tcgtggactg cggtgcggac
     1741 cggctcggcc acggcgtgcg catcgtcgac gaggtggggt tcgacggtga cggcgcggtg
     1801 gcactcggcc ccgtggcaca gatggtgcgc gacagtggca cctgcctcga gctgtgcccg
     1861 acgtccaacg tgcacaccgc cgcggccggt gtcatgagcc tggccgacca ccccatcgcc
     1921 ctgctggccg actgcgggtt cgcggtcacg atcaacaccg acaaccggct catgagcgac
     1981 gtgacggtgc tcgacgagta ccgcaggctc gccgagacgt tcggctgggg tgacgccgag
     2041 ttcaatgcgg cgaaccggac cgcgctgcgc accgccttct gcgacgacgc cacccgcgcc
     2101 cgtctcgccc cccgcttcga ctgagcgcag gtggccgagg ccgtttgtta cagccgggcg
     2161 gggtgccaga cggtcttgat ctcgaggaac tcgccagccg tgcgcgggct gcgcaccccg
     2221 tcggtgcgag cgacccgttt gaggttgtcg gccgatcgga cggtcaggtc gatcgccagc
     2281 tcggtgtcga cgacaccgga gatgtcgagc gcgtcgacat cgtgatgatc ggccaaccac
     2341 ggcgccagct cggcgaccgg gccgacgagc aggttggcga cccccgccgg aacgtcggac
     2401 gtagccagta cctcgccgaa cgtgagcggt gcgaagggcg acgcctccga ggcgagcacg
     2461 actgcggtgt taccgccgca caacaccggc gccaacaggg ccgacagacc cgagagggga
     2521 cgttcggtcg gagcgatcac gcccacgacg cccgtcggct ccggagtcga gacgttcagg
     2581 aacggccccg ccacggggtt cacgccaccg agcacctggg tcagcttgtc ggtccagccc
     2641 gcccagtaca cccacgtgtc gacggcgtcg tcgacctcga ccgcggcgtc gtaggtgtcc
     2701 acgccgccgt tctcgagctc ggcgacgaac tggtcgcgcc gaccgtcgag catctcggcg
     2761 atccggtaga ggatctgccc acgcaggtac ggcgtggccg cgccccaacc cgactgggcg
     2821 gcgcgggcct tcacgaccgc gtcgcggaga tccttgcgag acgcctgggc gacccgcacc
     2881 gtgccgccgt cgacgccggc cacggggtac acccgacccg actcggaacg gatgaacgcc
     2941 ccgccgctgt acaacttgta ggtcttggga gcaccgagtt cggtcacgca cccactccga
     3001 tccggtcgac gcccagcggc gagaggtcga gataggcctc gaggccgtgg cggcccccct
     3061 cccggccgaa gcccgactcg cgcatgccgc cgaacggact ggtcgggtcg aactggttgt
     3121 aggtgttggc ccacacgaca ccggcccgca gctttcgact catccacaag atgcgcgagc
     3181 cgctctcggt ccacacgccg gctgccaggc cgtagggcgt cgcgttcgcc ttggcgacgg
     3241 cttcttcggg cgtgcggaag gtcatgacgg cgaggaccgg cccgaagatc tcctcggtgg
     3301 ccacccggtg gctggtgtcc acaccgctga ggatggtcgg cgggtagaac caccccgtct
     3361 cgggcagcgg gcagctcgac tgcacgatct ccgcgccctc gtcgacgccg gccgccacga
     3421 gttccacgat ccgatcgagc tgctcacgcg agttgatggc cccgatgtcg gtgttcttgt
     3481 cgagcgggtc gccgatgcgc agcagctcca tacgacgacg cagcttctcg aggaactcgt
     3541 cggcgatgga ctcctgcaac agcaaccggc tgccggcgca gcacacgtgg ccctggttga
     3601 accagatgcc gcggaccacc ccttcgacgg cctgatcgat cggtgcgtcg tcgaagatca
     3661 cgtgcgccga cttgccgccg agctcgagcg tgacgcgggc gcctgtcgct gcggtggccc
     3721 ggcggatcgc ccggccgacc tcggtcgagc cggtgaaggc caccttgtcg acaccacgat
     3781 gggagctgag cgcggccccc gtggctccgc ccccggtgac gatgttcacc acgcccggag
     3841 gcaggtcggc ctcggcgatc acctcggcga gcagcagcgc ggtgagcgac gtcg
//
LOCUS       DAOSOA010000315         3886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_23489_length_3886_cov_7.024798, whole genome shotgun sequence.
ACCESSION   DAOSOA010000315 DAOSOA010000000
VERSION     DAOSOA010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23489_length_3886_cov_7.024798"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PKY13_14830"
     CDS             complement(<1..1129)
                     /locus_tag="PKY13_14830"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOT81267.1"
                     /translation="MTASAGTTPVPAEARGWESLRDSAAQIRRALDDQGNGSDFSHPT
                     LDDDGFPTIAHPDDTGSRLQPRRELDPVPLVVDAQDWARLGRALEQRAKVLELVLDDL
                     FGPQRLLAGGHLPTQAILTHREYLRGCVGIASPGSHRLVLYAADIARSTTGAVTVTAD
                     RTQMPSGIGHSVQNREVMARIHPESLHEAGVLPIEDWLDACRAALGALAPADIEQPRI
                     VMMTPGASVRTHTEHAYLARTMGFTLVEPSDLTVRDAKVWLKSVAGLEPVHVILRLVP
                     SPDCDPLELWTDSVLGVAGLVEACRRQNVAVANPLGSGVGECAALLPFMPRLTRTLLG
                     EEPLIESAPTWWCGEATGLSHVLANLDTLLIRSRDPVAGRHT"
     gene            complement(1126..2112)
                     /locus_tag="PKY13_14835"
     CDS             complement(1126..2112)
                     /locus_tag="PKY13_14835"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOT81268.1"
                     /translation="MLSRTAENLYWLGRHLERAEAVSRIIREHTKLIVDLPVDVESDW
                     AGLLSITGAVESYSSRYEQADELGVVSHLMADIGNPTSVIRTVAAARENLRVTRQLMP
                     ASLWECVSQLHSTVATSAGNCAARSSRLELATSIISASQQLVGILASTVSRDDAFSFY
                     ELGRNIERADMTTRVLDVRAAGLLSDGTRSGMPPADRSPYEDVRWMGVLRSLGAQHMY
                     TRTSVGSIQGASVVDFLVDDVDFPRAVSHCMGRIDQILAALPTRSMTIDRSHELKATI
                     AGRSRYTVDAAALRDWVDEVQLGIGALHTSLSSTFFTPETTRSLHPSGRVVV"
     gene            complement(2112..3656)
                     /locus_tag="PKY13_14840"
     CDS             complement(2112..3656)
                     /locus_tag="PKY13_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOT81269.1"
                     /translation="MLFSDYRSDTFDELIDVDGHPRDAAASLVKHLERMGIEEIEARQ
                     HAANLGIEAAGITFTVYTEGTGIDRAWPFDIVPRVISRRDWDRVDAGLVQRLTALNHF
                     IADVYAEQKIFADGIVPFDLIANSPGYRPECRGATPMHGVWAHICGSDLVRGKDGTIY
                     VLEDNLRVPSGVSYMLENREITKRVFPELFTEQSILPVDAYPERLRRILASLSPQGDQ
                     AVIGVLTPGIYNSAYFEHSFLARQMGAELVEGRDLFVEDEVLYMRTIHGPVRVDVLYR
                     RVDDAFLDPEVFRPDSALGTPGLMAAWRAGNLALANAPGTGVADDKAVYAYVPDMIRY
                     YLNQEPILSNVPTYQCGRDEDRAYVLDHLDELVVKPANESGGYGIIIGPKATAEELEQ
                     CAKEIRNDPRGFVAQPVIDLSVVPTLCGGELAPRHVDLRPFTLLGAESYVTCGGLTRV
                     AMVEGSLVVNSSQGGGSKDTWVVDTDAVAASPVSTTRGRSTTSTAADQSQSQSQTDLP
                     HPQGDT"
BASE COUNT          606 a         1251 c         1434 g          595 t
ORIGIN      
        1 gtgtgtggcg gcccgcaacc gggtcgcgtg accggatcag gagggtgtcg aggttggcga
       61 gcacgtgcga caggccggtg gcttcgccgc accaccacgt gggcgcgctc tcgatcagtg
      121 gctcctcgcc caagagcgtc cgggtgagcc gcggcatgaa gggaagcagg gcggcgcact
      181 caccgacacc ggagccgagc ggattggcca cggccacgtt ctggcggcga cacgcttcga
      241 ccagtccggc gacgccgagc accgagtcgg tccacagctc gagcggatcg cagtccggcg
      301 acgggacgag ccggaggatc acgtgcaccg gttccagacc ggccaccgac ttgagccaca
      361 ccttcgcgtc ccgcacggtg agatccgacg gctcgacgag cgtgaagccc atcgtccggg
      421 cgaggtacgc gtgctcggtg tgggtccgca ccgacgcccc cggcgtcatc atcacgatgc
      481 ggggctgttc gatgtcggca ggagcgagcg caccgagcgc ggcacggcac gcatcgagcc
      541 agtcctcgat cggcagcacg cccgcctcgt gcaacgactc ggggtggatg cgggccatca
      601 cctcgcggtt ctgcaccgaa tgaccgatgc ccgacggcat ctgggtgcgg tccgccgtga
      661 ccgtgaccgc ccccgtggtc gatcgggcga tgtcagcggc gtagaggacg agccggtggg
      721 atcccggcga cgcgatgccc acacacccgc ggaggtactc ccgatgggtc aggatcgcct
      781 gcgtggggag gtgcccgccg gcgaggagtc gctggggacc gaagaggtcg tcgagcacca
      841 gttcgaggac cttggcgcgc tgttcgagcg cccgaccgag ccgcgcccag tcctgggcgt
      901 cgacgacgag cggcaccggg tcgagctctc ggcgcggttg cagccgagac cccgtgtcgt
      961 cgggatgggc gatcgtcggg aagccgtcgt cgtcgagcgt ggggtggctg aagtccgacc
     1021 cgttgccctg gtcgtcgagc gcccgccgga tctgggcggc gctgtcccgg agcgattccc
     1081 acccccgtgc ctccgccggc acgggcgtgg tcccagcgct tgcggtcaca ccacgactcg
     1141 cccggagggg tggagcgaac gagtggtctc gggcgtgaag aacgtcgacg acagcgacgt
     1201 gtggagcgcc ccgatgccca gctgcacctc gtcgacccag tcgcgcaggg cggcggcatc
     1261 gacggtgtag cgcgaccggc cggcgatcgt cgccttcaac tcgtgggacc ggtcgatcgt
     1321 catcgagcgg gtcggcagcg ccgcgaggat ctggtcgatc cggcccatgc agtggctgac
     1381 ggcgcggggg aagtcgacgt catccaccag gaagtcgacg accgacgccc cctggatcga
     1441 gccgaccgag gtgcgggtgt acatgtgctg ggcgccgagg cttcgcagca cgcccatcca
     1501 acgcacgtcc tcatacgggc tccgatcggc gggcggcatg ccgctgcggg tgccgtcgga
     1561 caacagccct gcggcacgga cgtcgagcac tcgggtggtc atgtccgccc gctcgatgtt
     1621 ccggccgagc tcgtagaacg agaaggcgtc gtcgcggctg accgtggagg cgaggatgcc
     1681 gacgagctgt tggctcgccg agatgatcga cgtcgccagt tcgaggcggc tgctgcgcgc
     1741 cgcgcagttg ccggcgctcg tcgcgaccgt cgagtggagc tggctgacgc actcccacaa
     1801 cgacgccggc atgagctggc gggtgacccg caggttctcg cgagcggcag cgacggtgcg
     1861 gatgaccgac gtcgggttgc cgatgtcggc catgaggtgg ctgacgacgc cgagctcgtc
     1921 ggcctgctcg tagcggctcg agtacgactc gacagcgccg gtgatggaca gcaatccggc
     1981 ccagtccgac tcgacgtcga cgggcaggtc gacgatcagc ttcgtgtgct cgcggatgat
     2041 ccgggagacg gcttccgccc gctcgaggtg gcgtccgagc cagtagaggt tctctgcggt
     2101 gcgtgacaac atcacgtgtc tccttgcggg tgggggaggt cggtctgcga ttgcgattgg
     2161 gattggtctg cggcggtcga cgtggtcgac ctaccgcggg tggtcgacac cggggacgcc
     2221 gccacggcat cggtgtcgac cacccacgtg tccttggatc cgccaccctg ggacgagttg
     2281 acgacgaggg agccctcgac catcgcgacg cgggtcaggc cgccgcaggt cacatagctc
     2341 tccgcgccca acagggtgaa cggacggaga tcgacgtgcc ggggggccaa ctcgccgcca
     2401 cagagcgtcg gcacgaccga caggtcgatg acgggctggg cgacgaagcc acgggggtcg
     2461 ttgcgaatct ccttggcgca ctgctcgagt tcctcggcgg tcgcctttgg cccgatgatg
     2521 atgccgtagc cgcccgactc gttcgccggc ttcaccacca gctcgtcgag gtggtcgagc
     2581 acgtaggccc ggtcctcgtc gcgtccgcac tggtaggtgg gcacgttcga gaggatcggc
     2641 tcctggttga ggtagtagcg gatcatgtcg gggacgtagg cgtagacggc cttgtcgtcg
     2701 gcgacaccgg ttccgggggc gttggcgagc gccaggttcc cggcccgcca cgcggccatc
     2761 aacccggggg tacccagcgc ggaatccggt cgaaacacct cggggtcgag gaacgcgtcg
     2821 tcgacgcggc ggtacagcac gtcgacacgt accggcccgt ggatggtgcg catgtagagc
     2881 acctcgtcct cgacgaagag gtcgcggccc tcgacgagtt cggcacccat ctgacgggcg
     2941 aggaacgagt gctcgaagta cgccgagttg tagatgcccg gcgtgaggac cccgatgacc
     3001 gcctggtcgc cctgggggct gagcgacgcg aggatgcgcc gaaggcgctc ggggtaggcg
     3061 tcgacgggga ggatcgactg ctcggtgaac agctcgggga acacccgctt ggtgatctcg
     3121 cggttctcga gcatgtagga cacaccggag ggcacccgga ggttgtcctc gagcacgtag
     3181 atcgtgccgt ccttgcctcg gacgaggtcg ctgccacaga tgtgggccca cacgccgtgc
     3241 atcggggtgg cgccccggca ctcgggacgg tagccggggg agttggcgat gaggtcgaac
     3301 ggcacgatgc cgtcggcgaa gatcttctgc tcggcgtaga cgtcggcgat gaagtggttg
     3361 agggcggtca gccgttgcac gaggccggcg tcgacgcgat cccagtcgcg ccggctgatc
     3421 acccgcggca cgatgtcgaa cggccaggca cggtcgatgc cggtgccctc ggtgtagacg
     3481 gtgaaggtga tgccggcggc ctcgatcccg aggttcgccg catgctggcg ggcctcgatc
     3541 tcctcgatgc ccatccgttc gaggtgcttc accaaggagg cggcggcgtc acgcgggtgg
     3601 ccatcgacgt cgatgagctc atcgaaggtg tcggaccggt aatcgctgaa gagcacgccc
     3661 agagtgtggt gagcacgtgt gaagccacgg ttcgacccgt gttacggggt cgtgaacgac
     3721 gcgttcgttc ggccgacgcg gtcgaacccc ctccccctaa cgtggacttc atgcgaacac
     3781 gacgcgacgt actccggctg ggtgggggag cgggcttcat cgccgcgacc gctggatgga
     3841 tcaccgcgtg cgatccgggc ggtgtgaacc cgggaaccac gaccac
//
LOCUS       DAOSOA010000316         3868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_23626_length_3868_cov_6.008130, whole genome shotgun sequence.
ACCESSION   DAOSOA010000316 DAOSOA010000000
VERSION     DAOSOA010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23626_length_3868_cov_6.008130"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..2636
                     /gene="mobF"
                     /locus_tag="PKY13_14845"
     CDS             96..2636
                     /gene="mobF"
                     /locus_tag="PKY13_14845"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HOT81270.1"
                     /translation="MSIGKLAAGAGDYYTAMVADGAEEYFTSAREAPGVWVGQASQRL
                     GLAGTVQPDDFASVLDHQYPGTNVRITAARSAPSVAGFDATFCAPKSVSILHGLGTPD
                     IRAAVRQAHDVAVREALAVFESAASRGRRGHGGADVIDGDGFVAAAFAHRTSRAGDPH
                     LHTHVVIANLVHGPDGRWTALDARPLYHWSKTVGYLYEAELRHQLTRTVGVEWRTVRR
                     GIADVAEVPMQVVDEFSTRRHEIEAHLAENGFDSARSAQLAAYATRRMKDHTATPESL
                     VDTWQRRASELGFDCEQVCDRLNAGTLTLRWTGLVHLTPMFEHLAGPDGLTKTRSTFG
                     RREVIQGICDRLPIGAPVADICEWADLFIDSEHCTRLAGTKSAVIRTSDGRTVSARTD
                     ERRFSTPDMLTTERRLIAAAVGCADAGVGLADPQVVDVAIGQRPSMSDEQVEMVRQLC
                     TSGRGVDLVEGVAGAGKTFALAAANPAWTESGHTVIGCALAAKAARQLQTDADIPSRT
                     IDRLLIDLDRPERGGLAPNTVIVVDEAAMVGTRKVLRLLDHARQADAKVVLVGDPCQL
                     PEIEAGGAFVGLRQRLSASYLADNRRQREPWERAALARLRTGDTDTAIDTYIERGRVE
                     VAPTGAEARSRMIGDWLDARRTGTAVMLASRRVDVDALNRYARRALRKDGALGADKII
                     IAGHGYTEGDIVVALRNDRRLGLLNGTRATVDAIDTHAQVMRCRADDDQAVTIPFDYA
                     ADGHLTHGYATTIHKSQGATYDRCLILAGDHLTKESAYTAMSRGRIDNTLYLVDDGDR
                     ATDSHTNEIESSLVDRVRSSISRTAAQSMAVDQTPASHDDDLDFEIDL"
     gene            3199..>3868
                     /locus_tag="PKY13_14850"
     CDS             3199..>3868
                     /locus_tag="PKY13_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT81271.1"
                     /translation="MELVWPDRLSDCLAGRTAATIMTDITTTDPTTQSTGEEWRLPPP
                     ATGRVVVSDELAAQLVDQARADGVDLVGPGGLLGDLTKRVLEAGLEVEMADHVGYDKH
                     DPEGRNGANSRNGTRSKTVLTEVGPVEIDVPRDRDGTFDPKLVRKRQRRLAGVDEMVI
                     SLTAKGLTTGEVQAHLAEVYGTDMSREQVSKITDAILGEMAEWMTRPLERVYPVVFID
                     AMVVK"
BASE COUNT          696 a         1361 c         1228 g          583 t
ORIGIN      
        1 acggtgcctt ggtcaggtgc catttcgttc gacgcacttg caccacggtg ccctgtggtc
       61 ggacacgtcc gctgttcagg tcggcggtcg ccttgttgag catcggcaag ctcgccgccg
      121 gggccggcga ctactacacc gccatggtcg ccgacggcgc cgaggagtac ttcacctcgg
      181 cccgcgaggc ccccggcgtc tgggtcggtc aagccagcca acggctggga ctcgccggca
      241 ccgtccagcc cgacgacttc gcatccgtcc tcgatcatca gtatccggga accaacgtgc
      301 ggatcacagc ggctcggtcc gcgccgagcg tcgccgggtt cgacgcgacg ttctgcgctc
      361 cgaagtcggt gtcgattctc cacggcctcg gcacccccga catccgtgct gccgtccgcc
      421 aggcgcacga cgtcgccgtc cgtgaagcgc tcgcggtgtt cgagtccgca gcgtcgcgag
      481 gccggcgagg ccatggcgga gccgacgtga tcgatggcga cgggttcgtc gccgctgcgt
      541 tcgctcaccg cacctctcgc gccggcgatc cccacctgca cacacacgtg gtgatcgcca
      601 acctcgtcca cgggcccgac gggcggtgga ccgccctcga cgcccgaccg ctctaccact
      661 ggtcgaagac ggtcggctac ctgtacgagg ccgagctccg tcaccaactc actcggactg
      721 tcggtgtcga gtggcggacg gtgcggcggg ggatcgccga tgtcgccgag gtgccgatgc
      781 aggtggtcga cgagttctcc acccgccgtc acgagatcga agcacacctg gcggagaacg
      841 ggttcgactc cgcccgttcc gcacagctcg cggcatatgc cacccggcgc atgaaagacc
      901 acacggcgac acctgaatcg ctcgtcgaca cctggcagcg acgtgcgagc gagttggggt
      961 tcgattgcga acaggtctgc gaccggctca acgccggaac cctcaccctg aggtggacgg
     1021 gtctcgtcca tctcaccccg atgttcgagc acctcgccgg acctgacggg ctgaccaaaa
     1081 cccggtcgac gttcggacga cgcgaggtta tccaggggat ctgcgaccgc ctcccgatcg
     1141 gcgctcccgt cgccgacatc tgcgaatggg ctgatctgtt catcgactcc gaacactgca
     1201 cacgactcgc aggcaccaag tcggcggtca tccgaaccag cgacggccgg accgtgtcgg
     1261 cacgaaccga cgagagacgc ttctccaccc cagacatgct caccacggaa cggcgactga
     1321 tcgccgcggc cgtcggctgc gccgatgccg gggtcggcct cgccgaccca caggtcgtcg
     1381 atgtcgcgat cggacagcgc ccgtcgatga gcgacgagca ggtggagatg gtgagacaac
     1441 tctgcacgag cggtcgcggc gtcgacctcg tcgaaggtgt cgccggcgca ggcaagacgt
     1501 tcgcccttgc tgccgccaac ccagcctgga ccgagtcagg ccacacggtg atcggatgcg
     1561 ccctcgccgc caaagcggcg cgtcaactcc agaccgacgc cgacattccg tcccgcacca
     1621 tcgatcgact cctcatcgac ctcgaccgac ctgaacgcgg aggcctcgcc ccgaacaccg
     1681 tgatcgttgt cgacgaagcc gccatggtcg gtacccgcaa ggtcctccgt ctcctcgacc
     1741 acgcccgaca ggccgacgcg aaggtcgtgc tcgtcggcga cccgtgccaa ctccccgaga
     1801 tcgaagccgg cggagccttc gtcggactcc gccaacgcct ttcggcgtca tacctggccg
     1861 acaatcgccg ccaacgcgaa ccgtgggagc gcgccgcctt ggcccgactc cggaccggcg
     1921 acaccgacac agccatcgac acctacatcg agcgaggccg cgtcgaggta gcgcccaccg
     1981 gagccgaagc gcggtcgcga atgatcggtg attggctcga cgcacgccga accgggacgg
     2041 ccgtcatgct ggccagccga cgcgtcgatg tcgacgccct caacaggtac gcgcgtaggg
     2101 cactgcggaa ggacggcgcg ctcggtgccg acaagatcat cattgcgggc cacggctaca
     2161 ccgaaggcga catcgtggtg gcccttcgca acgatcgccg actcggccta ctgaacggca
     2221 caagagcgac cgttgacgcc atagacaccc acgctcaggt catgcggtgt cgcgctgacg
     2281 acgaccaggc ggtcacgatc ccgttcgact acgccgccga cgggcacctc acccacgggt
     2341 acgcgacgac gatccacaaa tcccaaggcg caacctacga ccggtgcctg atcctggccg
     2401 gcgaccacct caccaaggaa tccgcctaca ccgccatgtc ccggggacgc atcgacaaca
     2461 cgctgtacct cgtcgacgac ggtgaccgag ccaccgactc ccacacgaac gagatcgaat
     2521 cgtcgctcgt ggatcgcgtg aggtcatcga tcagccgtac tgctgctcag tcgatggcgg
     2581 tcgaccagac gccggcgagc cacgacgacg acctggactt cgagatcgat ctctgatcgc
     2641 gaccgtgccg atgatcggcg ggcgctgtga cgaccgggga ggttgtccct ggagtagcgg
     2701 tgttggctga tctcgtcgtg agttgagccc ctccggacga ggatgtgggt cttccacaac
     2761 tcaccgcctt acccgggagg gctcctgtct caagctagaa gccgtcaccc gagcgcgcct
     2821 ctgaccccgc tcgggcgccg ccgcacggtc gactgcgcct cgtcgaccgc tggtcgatcg
     2881 aagcgaccga gtggttcccg gtcgacccca cgaccgtccg gaagtggcgc gtcccgttcc
     2941 tcgccgaagg cgaaatcggc ctccgggacc ggtcctcacg cccgaaacgc gcactgaacc
     3001 ggactccgcg ccgagtacgc agcctgttga tccagctccg taagaggcgc cgttggggtg
     3061 cagaccggat agccatcaca tcgggctcgc cattcgacgg taaacacctc ttgcgccacg
     3121 ctggcctcgg acgactcgac agaggtgacc gcgccaccgc actaccggtg cgccgctacc
     3181 gggagtgtca gataaacggt ggaactggtc tggcctgaca ggctgagcga ctgcttggcc
     3241 ggaaggacag cagcaaccat catgactgac atcacaacaa cggatccgac gacccaatcg
     3301 accggagagg agtggcggtt gccgccgccc gcgacgggga gagtggtggt gagcgatgag
     3361 ctcgccgccc agctcgttga ccaggcccgc gcggatgggg tcgacctggt tggccctggc
     3421 ggacttttgg gggatctcac taagcgggtc ctcgaggccg gcctcgaagt agagatggcc
     3481 gaccatgtcg gctacgacaa acacgacccc gagggccgca atggcgccaa ctcgcgcaac
     3541 ggaacccgtt cgaaaacggt gctcaccgaa gtcgggcccg tcgagatcga cgtgccccgc
     3601 gatcgcgatg gcacgttcga cccgaagctg gtgcggaagc gtcagcgccg cctcgctggt
     3661 gtggacgaga tggtgatctc gttgaccgcg aagggtctca cgaccggtga ggtccaggcg
     3721 cacctcgccg aggtgtacgg caccgacatg agccgcgagc aggtctccaa gatcaccgac
     3781 gcgatcttgg gggagatggc cgagtggatg acacgccccc tcgaacgggt gtacccggtc
     3841 gtgttcatcg acgccatggt cgtcaaga
//
LOCUS       DAOSOA010000317         3842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_23823_length_3842_cov_4.841035, whole genome shotgun sequence.
ACCESSION   DAOSOA010000317 DAOSOA010000000
VERSION     DAOSOA010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23823_length_3842_cov_4.841035"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="PKY13_14855"
     CDS             complement(<1..1333)
                     /locus_tag="PKY13_14855"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HOT81272.1"
                     /translation="MSAVRTEDAMTATPGTTTNRYLEGLYAPIRTEYTEFELPVTGSL
                     PPELDGRYLRNGPNPFDEPDPATYHWFTGNGMVHGIRLRDGRAEWYRNRWIRSTGVSE
                     GLGEAPIPGVRHGGMEAANTNVIGMAGRTFAIVEAGGSPVELSDTLDSICFSDFDGTL
                     PNGFTAHPKRHPGTGRLHAVNYYWARPEVLEYVTVGADGRVERSIDIPVPGNPMVHDC
                     SITDSHLAVYDLPVTFNLEAAVAGAQLPYVWDDDYGARVGIMPLDGEAADVQWFEVDP
                     CYVFHPMNAHDDGDAVVLDVVRHPRMFDHNRLGPDEGVPTLWRWRLDRSTGTATEEQL
                     DDRGIEFPRVDERLVGRAHRYGWASSVTQSAVDAGQDLIGDSLVRYDVRSGAVDVIEF
                     GPGRSIGEAVFVPRADDAAEDDGWYLTLVHDRATDRSSLAVLDAGDPTAGPI"
     gene            1391..2005
                     /locus_tag="PKY13_14860"
     CDS             1391..2005
                     /locus_tag="PKY13_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT81273.1"
                     /translation="MARTADPAVRTALIEAAAQRLAVGGLDALSLRQVAADAGTSTQA
                     VYTHFGSKDDLVRAVVVEAFARLSAEVRSVPVTDDPLADLAAVSTAYRRNALANAHLY
                     RVMFELNPLALTDPAHHRAADAPPERLEEGLDAFWAMVEHAERCVAAGLLHGEPSELG
                     LLMWATAHGVVMLELAGFLGSDAEGVYDAATTATFTGLRQRPGA"
     gene            2133..2795
                     /locus_tag="PKY13_14865"
     CDS             2133..2795
                     /locus_tag="PKY13_14865"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT81274.1"
                     /translation="MAHDHHHSPAHDRPVEADAVSFWNDLYAASDQIWSGRPNHWLVD
                     LATDLTPGRALDLGSGEGGDAVWLAERGWSVTAVDVSTTALERAQAAAERSGVGAVIE
                     WERHDLAESFPTGDFDLVSAQFLQSPIDFPRDDVLRRAAAAVAPGGHLLVVAHAAMPA
                     WSNHTPPPGGFPTVEGTVDALDLGGEDWTIVVAEEREREGSGPDGHSGTLIDSVVFAR
                     RR"
     gene            2848..3678
                     /locus_tag="PKY13_14870"
     CDS             2848..3678
                     /locus_tag="PKY13_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT81275.1"
                     /translation="MSLTITLLGTGNPIPDALRAGPSTLVAGTGTTVLVDCGRGVVQR
                     LVAAGVFPPMLDAVLVTHLHSDHITDLNDVITTHWVMTMQPTPLRIYGPPGIGAVVDA
                     ILASLAPDISYRLAHHEDLTWEPLLEVTEVEAGASFPIGAASVTVGRTNHFPVEPAIG
                     FRFDQEGASVAIAGDSVPCEGLDALCAGADAYVQTVIRDDLVKLIPFPRLQDILDYHS
                     SVTDAAQTAARAGVGTLVLTHYVPAPAPDSLHEWVAIAAEHFAGPIVAGDDLTTVTVP
                     "
BASE COUNT          564 a         1389 c         1300 g          589 t
ORIGIN      
        1 cgatcggccc ggcggtgggg tcgccggcgt cgagcaccgc gagcgagctg cgatcggtcg
       61 cccgatcgtg caccagcgtc aggtaccagc cgtcgtcctc ggccgcatcg tcggcgcggg
      121 gtacgaacac ggcctccccg atggagcgac cgggcccgaa ctcgatgacg tcgacggcgc
      181 cagagcggac gtcgtagcgc accagcgaat ctccgatgag gtcctggccc gcgtcgaccg
      241 ccgactgggt caccgaggac gcccaaccgt accggtgagc tcgcccgacg agccgctcgt
      301 cgacgcgggg gaactcgatg ccccggtcgt cgagttgttc ctcggtcgcc gtgccggtcg
      361 agcgatcgag ccgccagcgc cacaacgtgg gcacgccctc gtcgggaccg aggcggttgt
      421 ggtcgaacat gcgagggtga cgcacgacgt cgagcacgac agcatcgccg tcgtcgtgtg
      481 cgttcatcgg gtggaagacg tagcacgggt ccacctcgaa ccactgcacg tccgcggcct
      541 cgccatcgag gggcatgatg ccgacgcggg cgccgtagtc gtcgtcccac acgtagggaa
      601 gctgggcgcc ggccaccgcg gcctcgaggt tgaacgtgac cggaaggtcg tacaccgcga
      661 ggtgggagtc ggtgatcgaa cagtcgtgca ccatcgggtt gccgggcacc ggaatgtcga
      721 tgctgcgctc gacccggccg tctgcgccga cggtcacgta ctccaacacc tcgggccggg
      781 cccagtagta gttgacggcg tgcaggcgcc cggtgccggg gtgacgcttg gggtgtgccg
      841 tgaacccgtt gggaagcgtg ccgtcgaagt cgctgaagca gatgctgtcg agggtgtcgc
      901 tgagctcgac gggactgcct ccggcctcga cgatcgcgaa cgtgcggccg gccatgccga
      961 tgacgttggt gttggccgcc tccataccgc catggcgaac gccggggatc ggcgcctcgc
     1021 ccagcccctc gctgacgccg gtcgagcgga tccaccggtt gcgataccac tcggcgcgcc
     1081 cgtcgcgaag gcggatgccg tgcaccatgc cgttgcccgt gaaccagtgg taggtcgccg
     1141 ggtccggctc gtcgaagggg ttgggaccgt tgcggaggta ccgaccatcg agctcgggtg
     1201 gcaacgagcc cgtgaccggc agttcgaact cggtgtactc ggtgcggatc ggggcgtaga
     1261 gcccctcgag gtagcggttg gtggtggtgc cgggcgtggc ggtcatggcg tcctcggttc
     1321 ggacagcgct cattccataa cgctgttgtg gaatctttct agtcctcgtc cggcatgctg
     1381 gcaagcagtt atggcccgca ccgccgatcc cgccgttcgc accgcgctca tcgaggcagc
     1441 ggcgcaacgc ctcgccgtcg gcgggctcga cgccctcagc ctgcgccaag tcgccgccga
     1501 cgccggcacg tcgacccagg cggtgtacac ccacttcggc agcaaggacg acctcgtccg
     1561 ggcagtcgtc gtcgaagcct tcgcccgcct gtcggccgag gtccgctcgg ttcccgtgac
     1621 cgacgacccg ctcgccgacc tcgctgccgt gtcgacggcg taccgtcgca acgccctcgc
     1681 caatgcccac ctctatcggg tcatgttcga gctcaatccg ctggcgctca ccgatccggc
     1741 ccaccaccga gccgccgacg ctccccccga acgcctcgag gagggcctgg acgcgttctg
     1801 ggcaatggtc gagcacgcgg aacgttgtgt cgccgcgggc ctgctgcacg gcgagccgag
     1861 cgaactgggc ctgctcatgt gggccacggc acacggcgtg gtgatgctgg agctcgccgg
     1921 cttcctcggc agcgatgccg aaggcgtcta cgacgccgcc accacggcaa ccttcaccgg
     1981 cctgcggcaa cgacccggcg cctgaccgct gcgccgtgga gtccgggcgt ggaggtcacg
     2041 tcggcgactc cacctcaccg cccgttcacc gtcatcttgc taattcagca tgaccacatg
     2101 tccccagcgc acctcgacgc gacactggcg acatggctca cgaccaccac cactccccgg
     2161 cccacgaccg tcccgtcgag gccgacgccg tgtcgttctg gaacgacctg tacgccgcga
     2221 gcgaccagat ctggagcgga cggccgaacc actggctcgt cgacctcgcg accgacctca
     2281 ctccgggccg agcgctcgac ctcggcagcg gcgaaggcgg cgacgccgtc tggttggccg
     2341 aacgagggtg gtccgtcaca gccgtcgacg tgtcgaccac ggcgctcgaa cgggcccagg
     2401 ccgcggcgga gcgctcggga gtcggggcag tgatcgagtg ggaacgccac gatctcgccg
     2461 agtcgttccc gaccggtgac ttcgacctcg tgtcggcgca gttcctgcag tcgccgatcg
     2521 acttcccccg tgacgacgtg ctgcgccgcg ccgccgccgc ggtcgctccc ggcggccacc
     2581 ttctcgtcgt cgcgcacgcc gcgatgcccg cgtggtcgaa ccacacacca ccgccgggcg
     2641 gtttcccgac cgtcgaaggc accgtcgatg ccctcgacct cggaggcgag gactggacga
     2701 tcgtcgtcgc cgaggaacgc gaacgcgagg gctcgggccc cgatggtcac tccggaaccc
     2761 tgatcgacag tgtcgtgttc gcccgccgac ggtgaccggc cgacgtacgc ggagctggcc
     2821 gaggcatcag ggtgccatga tcaggggatg tctctcacga tcaccctgtt gggtaccggc
     2881 aatccgattc ccgacgcgtt gcgcgccggg ccgagcaccc tggtcgccgg caccggcacg
     2941 accgtcctcg tcgactgcgg gcgcggcgtc gtgcagcgcc tggtcgctgc cggtgtgttc
     3001 ccgccgatgc tcgacgcggt gctggtgacc cacctccaca gcgatcacat caccgacctg
     3061 aacgacgtga tcacgaccca ctgggtcatg acgatgcagc ccactccgct gcggatctac
     3121 ggcccgcccg gcatcggcgc ggtcgtcgac gcgatcctgg cctcgctcgc gcccgacatc
     3181 agctaccggc tggcccacca cgaggacctg acgtgggagc cgctcctcga ggtcaccgag
     3241 gtcgaggccg gcgcctcgtt ccccatcggg gcggcgtcgg tcaccgtcgg tcggaccaac
     3301 cacttccccg tcgaaccggc catcgggttc cggttcgacc aggagggcgc gtcggtggcg
     3361 atcgccggcg actcggtgcc gtgcgagggc ctcgacgccc tgtgcgccgg cgccgacgcc
     3421 tacgtccaga ccgtgatccg tgacgacctg gtgaagctca tcccgttccc tcgcctgcag
     3481 gacatcctcg actaccactc gtcggtcacc gacgcggcgc agacggcggc acgggccggc
     3541 gtcggcacgc tcgtgctgac gcactacgtg cccgccccgg cgcccgactc gctccacgaa
     3601 tgggtggcga tcgccgccga gcacttcgcc ggaccgatcg tggcgggcga cgacctcacg
     3661 accgtcaccg tcccctgagg gttttgtact gcgagaccgg ccgctgacgg ctcgtctcgc
     3721 agtacaaaac ccccggggag ccggtgctac ttgatcaggg gatcgcgggg caggccgagg
     3781 atgcgctcac cgatctgatt gcgcttgatc tccgacgtgc cgccggcgat cgacatgccg
     3841 cg
//
LOCUS       DAOSOA010000318         3836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_23859_length_3836_cov_5.915366, whole genome shotgun sequence.
ACCESSION   DAOSOA010000318 DAOSOA010000000
VERSION     DAOSOA010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23859_length_3836_cov_5.915366"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKY13_14875"
     CDS             complement(<1..923)
                     /locus_tag="PKY13_14875"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT81276.1"
                     /translation="MTRPTIPTELAAIVGDAFVVVDPDLTARYRVDWTGRYGGADAVV
                     VRPGSTAEVAAVVAWAAARGVALVPQGGNTGLVGGSVPERAGSVVMSTERLVALGEVD
                     AVAGQVTVGAGVTPSMLADHVRGTGWRFAVDLGARDTATIGGMVATNAGGMRVFRHGS
                     MRAQVLGLEHVTAAGDVVSRLGGLQKDNTGFDLGSLLCGSEGTLGVVTAARLRLIPDP
                     AFRSTVLMGFESAPAARTAAWSLRRDVGPVEVIEYVSGRCVDMVAATFGLVAPFIAAH
                     VLLVEAAADVDPTDSLAAAIGNLAEPPTDVAV"
     gene            1019..2440
                     /locus_tag="PKY13_14880"
     CDS             1019..2440
                     /locus_tag="PKY13_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81277.1"
                     /translation="MPRPFRPHGAARHLTTAATIGAAGVLLGGLNLAPVAAQTDPTAT
                     TTPAADPAAPTDPAAPTTSVKPASTRPAPGRPTPSKDPEVLAADGRNPAGVIVATSPA
                     LDDVPVTSERYDGAAMQREADARDLADANHRITTNEERLAQLEVDRRALVELIGRTTE
                     RRRKLEVTAERVDVALRAIAVRRFVHSAGDDGYLDPTRTLDEQMEQSHLTKLAGEAEA
                     TVERQADSVQTAQARTAASLSEWEERLQTNEDDTATATRELTRARSDAEIATASIVGA
                     EEELRNARPMTVVAGSDLPLIALDAYWRAANDLRRTRPACGIAWWALAGIGRSESNHG
                     RSSGGQPAPDGTASVPVYGIPLDGTSGTRRITDTDRGALDLDPELDRAVGVMQFLPGT
                     WKRWAEDESGDKVADPQNIYDAALASGKLLCSSAPALDTDEGLRKAYFAYNRSDDYVE
                     KVLGRAKEYQALPVPDPVAAPSN"
     gene            complement(2444..2857)
                     /locus_tag="PKY13_14885"
     CDS             complement(2444..2857)
                     /locus_tag="PKY13_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81278.1"
                     /translation="MTDVIVVLVLISGFFVAVAVQRRRKAERRARATTVELVIDGDQV
                     RRRLGDGREESARWAEVISVEVVCTPVRTADGATAFVLIAEGAESGCLVPLGVGHDDA
                     LVQQLSRLRGFRTEQFVAATEHKPPRRTMVWEREG"
     gene            3050..>3836
                     /locus_tag="PKY13_14890"
     CDS             3050..>3836
                     /locus_tag="PKY13_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT81279.1"
                     /translation="MQRRSKRGFKFAAFGLALALTAGACGGDDKKSDEEGPDTTVPTT
                     DAPAGKPTPGGELTYSVEADASGGYCLPTAQLAAGGIQIANAVYDSLFVFDKDFKATP
                     YLAESSSWNPEYTSLTIKLRQGITFHDGTPLNAEIVKLNLDVSRGDAEATAKTGLSPL
                     LFIFVFQNIASIDAPDDSTVVINTKVPWPALPEFLASGRNGIVGMSQLMAGTDGCKDQ
                     FNGTGPFKVDSWTPNVEMKLSKNPNYWRKDADGVQLPYLDKLNF"
BASE COUNT          655 a         1474 c         1240 g          467 t
ORIGIN      
        1 acggcgacgt cggtcggagg ttccgccagg ttgccgatgg ctgcggcgag ggagtcggtc
       61 gggtcgacgt cggcggctgc ttcgaccagg agcacatgcg cggcgatgaa cggggccacg
      121 aggccgaacg tcgcggcgac catgtcgacg caccggcccg acacgtactc gatgacctcg
      181 accggcccga cgtcccgccg cagcgaccac gccgcggttc gcgccgccgg agcggactcg
      241 aaccccatca gcaccgtgga gcggaacgcc gggtcgggaa tcagccggag tcgcgccgcc
      301 gtcacgacgc cgagcgtgcc ctcggacccg cacaggagcg agcccagatc gaatcccgtg
      361 ttgtccttct gcaggccacc gagtcgggac acgacgtcgc cggcggccgt gacgtgctcg
      421 aggccgagga cctgggcgcg catcgaacca tggcggaaca cccgcatccc accggcgttc
      481 gtcgcgacca tgccgccgat cgtggcggtg tcacgggcgc cgaggtcgac ggcgaatcgc
      541 cacccggtcc cccgcacgtg gtccgcgagc atcgacgggg tcaccccggc cccgaccgtc
      601 acctgcccgg caaccgcgtc gacctcgccg agcgccacga gccgctcggt ggacatcacg
      661 accgaaccgg cgcgctcggg cacggagccg ccgaccagcc cggtgttgcc accctgaggg
      721 acgagcgcga ccccgcgggc cgctgcccac gccacgacag cggcgacctc cgccgtcgac
      781 cccggacgca cgaccaccgc atcggctccg ccgtagcgcc ccgtccagtc gacccggtag
      841 cgagcagtca ggtcgggatc gacgaccacg aacgcgtcac cgacgatcgc tgccaactcg
      901 gtggggatcg tcggcctcgt caccggacca gtctcgcacc agcgatccac gatccgggcc
      961 cgtccaccca ctagaagtgg gaggttcgcg gcaccccccg acgccgcccc cgaccgtcat
     1021 gccccgaccc ttccgcccgc acggcgccgc ccgtcacctc acgaccgccg cgaccatcgg
     1081 cgcggccggg gtgctgctcg gcgggctcaa cctggcaccg gtcgccgcgc agaccgaccc
     1141 gacggccacc acgacgccgg cggccgatcc cgcggcaccg acggacccgg ccgccccgac
     1201 caccagcgtg aaaccggcct cgacccggcc ggctcccggc cgccccacac cgagcaagga
     1261 tcccgaggtc ctggccgccg acgggcgcaa cccggcgggg gtcatcgtcg cgacgtcgcc
     1321 cgcactcgac gacgtgccgg tgacgagcga gcgctacgac ggcgccgcga tgcaacggga
     1381 ggccgatgct cgcgacctcg ccgacgcgaa ccaccggatc accacgaacg aggaacggct
     1441 cgcccaactc gaggtcgatc ggcgggcgct cgtcgagctc atcggtcgga cgaccgaacg
     1501 acgtcgcaag ctcgaggtca ccgccgaacg cgtcgatgtc gcgttgcgag ccatcgccgt
     1561 tcgccgcttc gtgcacagcg cgggcgacga cgggtacctg gacccgaccc gaacgctcga
     1621 cgaacagatg gaacagtcac acctgacgaa actcgccggt gaggccgagg ccaccgtcga
     1681 acgccaggcc gactcggtcc agacggcgca ggcccgcacc gccgcctcgc tctcggaatg
     1741 ggaggagcgc ctgcagacca acgaggacga caccgcaacc gcgacccgcg agctcacccg
     1801 tgcccgttca gacgccgaga tcgccacggc gtcgatcgtg ggtgccgagg aagagctccg
     1861 caacgcccga ccgatgaccg tcgtcgccgg cagcgacctt cccctcatcg cgctcgacgc
     1921 gtactggcgg gccgcgaacg acctgcggcg gacccggccc gcctgcggca tcgcctggtg
     1981 ggcgctcgcc ggcatcgggc gctccgagtc gaaccacggt cgatcgagcg gtggccagcc
     2041 ggcgcccgac ggcacggcgt cggtgccggt gtacggcatc ccgctcgacg gcaccagcgg
     2101 gacccgccgt atcaccgaca ccgatcgggg tgcgctcgac ctcgaccccg aactcgaccg
     2161 cgccgtcggg gtcatgcagt tcctccccgg cacctggaag cgctgggcgg aggacgagtc
     2221 gggcgacaag gtcgccgacc cccagaacat ctacgacgcc gcgctcgcgt cgggcaagct
     2281 cctgtgcagc tcggcgccgg cgctcgacac cgacgagggt ctgcggaagg cgtacttcgc
     2341 gtacaaccgc agcgacgact acgtcgagaa ggtgttgggg cgggccaagg agtaccaggc
     2401 gctgccggtg cccgatcccg tcgcggcacc gtcgaactga ccgctacccc tcccgctccc
     2461 acaccatggt ccgccggggc ggcttgtgct ccgtcgccgc cacgaactgc tccgtgcgga
     2521 aaccgcggag gcggctcagc tgctggacca gcgcgtcgtc gtggccgacg cccaacggca
     2581 ccaggcaccc cgactcggca ccctccgcga tcagcacgaa cgccgtcgcc ccgtctgcgg
     2641 tccgtaccgg ggtgcacacg acctcgacgc tgatgacctc ggcccagcgc gccgattcct
     2701 cccgtccgtc gccgaggcga cgccgcacct ggtccccgtc gatgacgagc tcgacggtgg
     2761 tggcccgcgc tcgccgctcg gccttgcgtc gtcgttggac cgccacggcg acgaagaacc
     2821 cactgatcag gacgagcacc acgatcacgt cggtcacgcc gagatcgtag ggctgcgggc
     2881 cgccgccgat tcccgcatga cgaactcgtg acatgggacg aacgccaact gatcaagcgc
     2941 tacagtgctg cggcaaaccg tgtgtcgggg gtcacaggta agcgttttca cgactcccct
     3001 cccacttgat cgaagccgtc cagcggctcg gaaccatgga gaagtcatcg tgcaacgacg
     3061 ttctaagcgg gggttcaaat ttgccgcatt cggactcgca ctcgccctga ccgcaggcgc
     3121 ctgcggtggg gacgacaaga agtccgacga ggaggggccc gacaccacgg tgccgaccac
     3181 cgacgctccg gccggcaagc cgaccccggg cggcgaactg acctactcgg tcgaggccga
     3241 cgcgtccggt ggctactgcc tcccgaccgc gcagctcgcc gccggcggca tccagatcgc
     3301 caacgccgtc tacgactcgc tgttcgtgtt cgacaaggac ttcaaggcca cgccgtacct
     3361 ggccgagtcg tcgagctgga accccgagta cacgtcgctc accatcaagc tgcgccaggg
     3421 catcacgttc cacgacggca ccccgctcaa cgccgagatc gtcaagctga acctcgacgt
     3481 cagccgcggc gacgccgagg ccaccgcgaa gaccggcctg tcgccgctcc tgttcatctt
     3541 cgtgttccag aacatcgcca gcatcgacgc acctgacgac tccaccgtgg tgatcaacac
     3601 caaggtgccg tggccggcac tgcccgagtt cctggcgagc ggccgcaacg gcatcgtcgg
     3661 catgtcccag ctgatggccg gcaccgacgg ctgcaaggac cagttcaacg gcaccggccc
     3721 gttcaaggtc gacagctgga cgccgaacgt cgagatgaag ctctcgaaga acccgaacta
     3781 ctggcgcaag gacgccgacg gcgtgcagct cccctacctc gacaagctga acttca
//
LOCUS       DAOSOA010000319         3802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24148_length_3802_cov_4.659995, whole genome shotgun sequence.
ACCESSION   DAOSOA010000319 DAOSOA010000000
VERSION     DAOSOA010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24148_length_3802_cov_4.659995"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1090
                     /locus_tag="PKY13_14895"
     CDS             104..1090
                     /locus_tag="PKY13_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT81280.1"
                     /translation="MRSGLRLPAAVVAFACVAAVGCTTDDDGGDTTAAAVVTTTTVAP
                     AAPASDDCRNLPPTASYPPLVSLPAPNQMPTGSKLREIQDRGKLTVGVSADTLLFGAQ
                     NPLTGVIEGFDIDMLNLVASAIFGDDDDRLDIKVITYAQRIPSLQDRSVDLVAHTMTI
                     NCTRWNQVGFSSTYYAAGQKILVRRDSTKTSIADLDANGAEVCAPSGSTNIDELANYP
                     GVKVRAVDDITDCLVLFQQGKVEAITADDTVLAGFADQDPYAVVVGDAFTQEPYGIAA
                     NAEDVQLVQFVNKVLANARDDGRWKAIYERWLGAFGPTPEPPAAEAQLRPLP"
     gene            1087..2292
                     /locus_tag="PKY13_14900"
     CDS             1087..2292
                     /locus_tag="PKY13_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81281.1"
                     /translation="MSTAGVTGSYPAVGSEIARAEYDALLETLSAWINDRGQRLVELD
                     ERSVAAEASQRTDVGLAFVLWQAISERRDELVGIDWRDHQRPNGEVATLVWRGIDDRT
                     GAPLAADLAEAVALVDELIRRVAGALDHSDTSNVARHEARMSVETDLAVAERLAAELG
                     DQVRAAADLRRELTRLTSSPVSGGASSTPELDDAALAELRAAAARLRVDLEATDRERR
                     AALDACSAATASLDRLRALESDARAAVAECVDRFADPPRLGVPSVDAVEVPPADLAGR
                     PWPQARPMAEAFLRTVDRLERALRHVIDVNTAPRRERDDLRGLLQAYRDKADAHGAAE
                     RPDVDAAYRAARDVLWSAPCDLGRARELTTDFQRLVGAVTGSAASDGSNGAPSDGPAG
                     RPVDETEAP"
     gene            2289..>3802
                     /locus_tag="PKY13_14905"
     CDS             2289..>3802
                     /locus_tag="PKY13_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOT81282.1"
                     /translation="MTACVVPGCGGTIQDGYCDRCGIAASTAAAPAAGAGAAASGTAP
                     GELSTSVSARLGSTPIGSRRSEPGTRPTRRIGASRSARSRLGAGFTTVPSAPVLDPVA
                     ALMDPPEVPERKRNCANCGAPVGRSRGDQPGRSTGFCPKCRTPYDFDPKLRPGTVLGG
                     QYEVMGCLAHGGMGWIYLARDRNVNDRWVVLKGLLNAGDPDAFRAAVAEKQYLAEVEH
                     PLIVEIYNFETHSDGSSYIVMEYVGGKSLSDILKAKMAERGGEFQALPPDQAIAFMIE
                     VLPAFAYLHAAGLLFCDFKPANLIQVADTIKLIDLGGVRRVDDDLSPIYGTVGYQAPE
                     VPSDGCSVPSDLFTVVRTLAMLVFEFKGYQQQYLASMPPQETAPVLVEHDSLYRLLVK
                     GMAANPEDRFQSADELRDQLVGVLREVVALAAPGAGAAAGSSPSTLFEAPGSATEVAS
                     WDDLPRLRSDPDDPMATWIAGVTVADPVRRIDVLRTAPQTTPGVRQALAYAGLEA"
BASE COUNT          546 a         1404 c         1308 g          544 t
ORIGIN      
        1 cccgcgacag cgtgtcgggc catcggtggc tcgtgctcgt ggcggggttc gttgcagcgg
       61 ccgctgcgtt cgcggggttc gcccgtcgca tccaggagta ccgatgaggt cggggctgcg
      121 tcttcctgcc gccgtcgttg cgttcgcgtg cgtcgccgcg gtcgggtgca cgaccgacga
      181 tgatggcggt gacaccacgg cggccgctgt cgtcaccacg accacggtcg cgccggcagc
      241 gccggcgtcg gacgactgcc gcaacctccc gccgacggcg tcgtacccgc cactcgtctc
      301 cctgcccgct cccaaccaga tgccgacggg gtcgaagctc cgcgagatcc aggaccgcgg
      361 gaagctcacc gtcggcgtgt cggccgacac gctcctgttc ggcgcccaga accccctcac
      421 gggcgtcatc gagggcttcg acatcgacat gttgaacctc gtggcctcgg ccatcttcgg
      481 ggacgacgac gaccgtctcg acatcaaggt catcacctac gcccaacgca tcccgtcgct
      541 gcaggaccgg tcggtcgacc tcgtcgccca cacgatgacg atcaactgca cccggtggaa
      601 ccaggtgggg ttctcctcga cgtactacgc ggcgggccag aagatcctcg tgcgccgtga
      661 ctccaccaag acgtcgatcg ccgacctcga cgcgaacggg gccgaggtgt gtgcgccgtc
      721 cggttccacc aacatcgacg aactcgccaa ctacccgggg gtgaaggtgc gggcggtcga
      781 cgacatcacc gattgcctcg tgctcttcca gcagggaaag gtcgaggcca tcaccgccga
      841 cgacacggtc ctcgccgggt tcgccgacca ggacccgtac gcggtggtcg tcggcgacgc
      901 gttcacccag gagccctacg gcatcgcggc gaacgccgag gacgtgcagc tcgtgcagtt
      961 cgtcaacaag gtgctcgcga acgcccgcga cgacggccgg tggaaggcga tctacgagcg
     1021 ctggttggga gcgttcggcc ccacccccga accgcccgca gccgaagccc agctgcggcc
     1081 gctcccatga gcaccgccgg tgtgaccggg tcgtaccccg ccgtggggtc ggagatcgct
     1141 cgggcggagt acgacgccct gctcgagacg ttgtccgcgt ggatcaacga ccgtggtcaa
     1201 cggctcgtcg agctcgacga gcgctcggtc gctgcggagg cgtcgcagcg gaccgacgtc
     1261 ggtctggcgt tcgtcctgtg gcaggcaatc tcggagcgcc gcgacgaact cgtcgggatc
     1321 gactggcggg atcaccagcg ccccaacggc gaggtcgcca ccctggtctg gcgcgggatc
     1381 gacgaccgta cgggcgctcc cctcgccgcc gatctcgccg aggccgtggc gctcgtcgac
     1441 gaactgatcc gccgggtggc cggagcgctc gaccacagcg acacgtccaa cgtcgcccgc
     1501 cacgaggccc ggatgtcggt ggagaccgac ctggccgtcg ccgagcggct ggcagcagag
     1561 ctcggcgacc aggtgcgcgc cgcggccgac ctgcgccggg agttgacgag actcacctcg
     1621 tcgccggtgt cgggcggagc gtcgtcgacc cccgaactcg acgacgccgc gctcgccgag
     1681 ctccgcgctg ccgcggcccg gttgcgcgtc gacctcgagg cgaccgaccg cgaacgtcgc
     1741 gccgccctcg acgcgtgcag cgcggcgacg gcctcgctcg accgacttcg tgcgctcgaa
     1801 tcggacgccc gcgccgcggt cgccgagtgc gtcgaccgct tcgccgatcc gccccggctg
     1861 ggcgttccct ccgtcgacgc cgtggaggtg ccgcctgccg acctggcggg tcgaccatgg
     1921 ccgcaggcac gccccatggc cgaggccttc ctccgaaccg ttgatcgtct ggagcgcgcc
     1981 ctgcgccacg tgatcgacgt caacacggca cctcggcgtg agcgcgacga cctgcgcgga
     2041 ctcctccagg cctaccgcga caaggccgac gcccacggtg cggcggagcg gcccgacgtc
     2101 gacgcggcct accgcgctgc tcgcgacgtg ctctggtccg cgccgtgcga cctgggccgt
     2161 gcccgcgagc tgaccaccga cttccagcgg ctcgtcgggg ccgtcacggg ctcggcagcg
     2221 agcgatggct cgaacggcgc accgagcgac ggaccggccg gccgaccggt cgacgagacg
     2281 gaggccccat gaccgcatgc gtcgtccccg gctgcggcgg cacgatccag gacggctact
     2341 gcgaccgatg tggcatcgcc gccagcacag ccgccgcccc tgctgcggga gctggtgctg
     2401 ccgcgagcgg cacggccccg ggcgagctgt cgacgtcggt ctcggcccgc ctcggctcga
     2461 caccgatcgg cagccggcgt tccgaaccgg gcacccgacc gacccgccgc atcggcgcgt
     2521 cccgcagtgc ccgctcccgg ctcggcgccg ggttcacgac agtgcccagc gccccggtgc
     2581 tcgaccccgt cgcggcgctc atggatccac cggaggttcc ggaacgcaag cggaactgcg
     2641 ccaactgcgg tgcgccggtc gggcgcagcc gcggtgacca acccggtcgg tccacggggt
     2701 tctgccccaa gtgccgcacc ccctacgact tcgaccccaa gcttcggccg ggcaccgtgc
     2761 tcggcggcca gtacgaggtg atggggtgcc tggcgcacgg tggcatgggg tggatctacc
     2821 tggcccggga tcgcaacgtg aacgaccggt gggtcgtgct caagggcctg ctcaacgctg
     2881 gtgaccccga tgcattccgt gccgcggtcg ccgagaagca gtacctcgcc gaagtcgagc
     2941 atccgctcat cgtcgagatc tacaacttcg agacgcactc cgacggcagc tcgtacatcg
     3001 tgatggagta cgtcggcggc aagagtctgt ccgacatcct gaaggcgaag atggcggagc
     3061 ggggcggcga gttccaggcc ctgccgcccg accaggccat cgcgttcatg atcgaggtgc
     3121 tgccggcgtt cgcgtacctc cacgccgccg gcctgctgtt ctgcgacttc aagccggcca
     3181 acctgatcca ggtggccgac accatcaagc tcatcgacct gggcggggtg cgccgcgtcg
     3241 acgacgacct gtcgcccatc tacggcacag tcggctacca ggcgcccgag gtgccgagtg
     3301 acggatgctc ggttccgtcc gatctgttca ccgtcgtccg cacgctggcg atgctggtgt
     3361 tcgagttcaa gggctaccag cagcagtatc tggcgtcgat gccgccccag gagacggcgc
     3421 cggtgctcgt cgagcacgac tccctctacc gactcctcgt gaagggcatg gcagccaacc
     3481 ccgaggatcg gttccaatcg gccgacgagc tccgcgacca gctggtaggg gtgctgcgcg
     3541 aggtcgtcgc gctcgctgcg cccggggccg gcgccgcggc gggatcgagc ccgtccacgc
     3601 tgttcgaggc gccgggctcg gcgaccgagg tggcgtcgtg ggacgacctg ccacggctgc
     3661 gctccgatcc cgacgatccg atggccacgt ggatcgccgg cgtcaccgtc gccgatccgg
     3721 tccgccggat cgacgtgctg cgcaccgcgc cgcagacgac gccgggggtt cggcaggcgc
     3781 tcgcctacgc cgggctcgag gc
//
LOCUS       DAOSOA010000320         3800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24167_length_3800_cov_6.448598, whole genome shotgun sequence.
ACCESSION   DAOSOA010000320 DAOSOA010000000
VERSION     DAOSOA010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24167_length_3800_cov_6.448598"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PKY13_14910"
     CDS             <1..1188
                     /locus_tag="PKY13_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOT81283.1"
                     /translation="GGDPEMLARIATAGSRRVDGHAPGLRGQQLDAYLAAGVESDHEC
                     TMLDEATEKRMKGMWVFIRQGSASKNLDDLIPMVLEGGTDLIAFCTDDREPDTLRSVG
                     HINDCCRRAVAAGVSTEDALIMASTNPALYHGFTHLGSLAPGYQADILAFDDLGSWQP
                     TTVWQRGRVVATRGEIAPGLIEDTPAPEWMKSSIHLETALDAADFALAAPEGDAKVIG
                     VVGGSLTTAHLTMDVDDAANDVARIACVERHRETGRVGLGYVSGFGIERGAIASTVAH
                     DAHNCMAIGHRGPSGPAEMAAAVNRLREIGGGQVVVLDGEVLAEVRLPIGGLMSDRLA
                     GEVAAELDTLVEAGRTLGITIEAPFMQLSFLGLSVLPQLRITDRGLVDVDRFELTSLA
                     AGD"
     gene            complement(1233..1895)
                     /locus_tag="PKY13_14915"
     CDS             complement(1233..1895)
                     /locus_tag="PKY13_14915"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOT81284.1"
                     /translation="MPDSIRLAAVNVGTPKVLADHGGDRVWSGIGKRPLPPDTTLFLS
                     LVNLAGDGQADLTVHGGIDKAVYAYPSEHLPAWAAELGEALGDAPFGENLSTIGVTES
                     DVRIGDRWEWGEALLEVCQPRSPCFKLALHRRRADIQQLFRQSGRVGWYLRVLHPGAV
                     STGSDISVIERDAAGLTVADAHRAMGDRRLEDRTLVEALAAHDRLADEWRLPLVERLA
                     RR"
     gene            1936..3135
                     /locus_tag="PKY13_14920"
     CDS             1936..3135
                     /locus_tag="PKY13_14920"
                     /inference="COORDINATES: protein motif:HMM:NF025460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4272 domain-containing protein"
                     /protein_id="HOT81285.1"
                     /translation="MAFEPTPAVVYSPEPDLGAVLDVLRSTPGLLDLRADGPDDDWRE
                     IRLTIDDPNAVRRVLVRNDPSHWQRPGFDTQLAGMQQFLARHGVDARTGPMIESLSEL
                     GFAISLSGDDGQRHPYLHDDDPAGRLTKALAHRLDGVVFWISGLFDADALLLLGGTGQ
                     HPQATPPDFMADPPSPSRVARRFLVLLALHLRAQLEYLDEPSIDREYERSRLLDWIAT
                     GGFAEEFEPHETSPHVPCGELSAQPTIDDWWRLEGAVLLAWALGLAPPPSDAPSDAAE
                     ELATIAGWPNGVPAGLVDGATMRSDDELRAAQTHQFFWHWRFVDHRVNPRALDFAELG
                     RSSWFGNFADGEFRLVDGDLAVGDRSISDADPNIVAGHASAAAERHTAFNWLRGGRSY
                     GDTQANT"
     gene            3219..>3800
                     /locus_tag="PKY13_14925"
     CDS             3219..>3800
                     /locus_tag="PKY13_14925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HOT81286.1"
                     /translation="MTPIDLAPAGLRMAALVAAVPEELLGAPTPCPAYCVGDLLDHIA
                     GLGTAFSAAARKDAGADSEPAPPGDRSRLSDDWRSDIAAGLSELADAWRPPAAWSGMT
                     RVGGVVLPGEVCGLVGLNELVVHAWDLGRATGQPVNAEPEVIRALLPMLDQFSPPIDA
                     PRGDGPFGPRFPVSSGAPLLDQLVGLAGRDPSWS"
BASE COUNT          523 a         1434 c         1289 g          554 t
ORIGIN      
        1 ggcggcgacc ccgagatgct ggcccgcatc gcgacggccg gaagtcgtcg cgtcgacggc
       61 cacgccccgg gcctccgcgg ccaacagctc gacgcctacc tcgccgccgg cgtcgagtcc
      121 gatcacgagt gcacgatgct cgacgaggcc accgagaagc gtatgaaggg catgtgggtg
      181 ttcatccgtc agggttcggc gtcgaagaac ctcgacgacc tcatcccgat ggtgctcgag
      241 ggtggcaccg acctgatcgc cttctgcacc gacgaccgcg agcccgacac gctgcggtcg
      301 gtcggccaca tcaacgactg ttgccgccgc gccgtcgccg ccggtgtgtc gaccgaggac
      361 gcgctcatca tggcgtcgac gaatcccgcg ctctaccacg gcttcaccca cctgggctcg
      421 ctggcgccgg gctaccaggc cgacatcctg gcgttcgacg acctcggctc gtggcagccg
      481 accaccgtct ggcagcgcgg gcgagtcgtc gcgacccgag gcgagatcgc ccctggtctc
      541 atcgaggaca cgccggcgcc cgagtggatg aagtcgtcga tccacctcga gaccgcgctc
      601 gacgcggccg acttcgcact cgctgcaccc gagggcgacg ccaaggtcat cggggtcgtc
      661 gggggttcgc tcaccacggc gcacctcacg atggatgtcg acgacgctgc caacgacgtc
      721 gcccgcatcg cgtgcgtcga gcggcaccgc gagaccggtc gggtcggcct cggttacgtg
      781 agcggcttcg gcatcgagcg cggcgccatc gcatccaccg tcgcccacga cgcccacaac
      841 tgcatggcga tcggccaccg cggtccgtcc gggccggccg agatggctgc agcggtcaac
      901 cggctccggg agatcggtgg cgggcaggtc gtggtgctcg acggcgaggt gctcgccgag
      961 gtgcggctgc cgatcggcgg gctcatgtcg gatcgtctcg ccggcgaggt cgccgccgag
     1021 ctcgacacgc tggtcgaggc cggtcggacc ctcgggatca cgatcgaggc gccgttcatg
     1081 cagctcagct tcctgggcct gtcggtgttg ccccagctcc gcatcaccga ccgcggcctc
     1141 gtcgacgtcg accgcttcga actcacgtcg ctcgccgccg gcgactgagg gccaccactc
     1201 cggcggccgg gctgtcgaac ggttgggagc gtctaccgcc gggcgagccg ctcgacgagc
     1261 ggcagccgcc attcgtcggc cagccgatcg tgcgctgcca gcgcctcgac cagcgtccgg
     1321 tcctccaagc ggcggtcccc catcgcccgg tgggcgtcgg caacggtgag accggccgca
     1381 tcgcgctcga tcaccgagat gtccgatccg gtcgacaccg cgcccgggtg cagcacccgg
     1441 aggtaccacc ccacgcgacc cgactgacgg aagagctgct ggatgtcggc gcgccgccgg
     1501 tgcagcgcga gcttgaagca cggcgaccgt ggctggcaca cctccaggag cgcctcgccc
     1561 cactcccacc gatcgccgat gcggacgtcg gattcggtga cgccgatcgt gctgaggttc
     1621 tcaccgaacg gtgcgtcgcc gagcgcttcg ccgagttcgg ccgcccacgc cggcaggtgc
     1681 tcggagggat aggcgtacac ggccttgtcg atcccgccgt gcaccgtgag atcggcctgc
     1741 ccgtcaccgg cgaggttcac gagcgacagg aacagggtgg tgtcgggcgg cagcggccgc
     1801 ttgccgatcc cgctccacac gcggtcgccg ccgtggtccg cgagcacctt cggtgtcccg
     1861 acgttgaccg ccgcgagccg gatcgaatcg ggcatgtcct caaactagcg accgtccggt
     1921 acctagactc ccgtgatggc gttcgagccg acacccgccg tggtgtacag ccccgagccc
     1981 gatctcggtg ccgtcctgga cgtgctgcgg tccacccccg ggctgctcga cctccgagcc
     2041 gatggtcccg atgacgattg gcgggagatc cgcctcacga tcgacgaccc caacgccgtc
     2101 cgccgcgtgc tcgtgcgcaa cgacccgagc cactggcagc gaccggggtt cgacacccag
     2161 ctcgccggga tgcagcagtt cctcgcccgt cacggggtcg acgcccgcac ggggccgatg
     2221 atcgaatcgc tctcggagct cggattcgcg atctccttgt cgggcgacga cgggcagcgc
     2281 cacccgtacc tccacgacga cgatccggcc ggccgcctga cgaaggcgct cgcccaccgg
     2341 ctcgacggcg tcgtgttctg gatctcgggc ctcttcgacg ccgatgccct cctcctgctc
     2401 ggcggcactg gccaacatcc gcaggcgaca ccaccggact ttatggccga tccgccgtcg
     2461 ccgtcgaggg tggcccgccg gtttctcgtg ctcctcgccc tgcacctccg ggctcagctc
     2521 gagtacctcg acgagccgtc gatcgatcgg gagtacgagc gctcccgcct cctcgactgg
     2581 atcgcgaccg gaggtttcgc ggaggagttc gagccgcacg agacctcgcc gcacgtgccc
     2641 tgcggcgagc tgagcgccca accgacgatc gacgactggt ggcgcctcga aggcgctgtt
     2701 ctgctggcgt gggcgctcgg tctggccccg cccccgtccg acgctccgag cgatgccgcc
     2761 gaggagctcg caacgatcgc cgggtggccc aacggggttc cggccggact cgtcgacgga
     2821 gccacgatgc ggagcgacga cgagcttcgt gcggcgcaga cccaccagtt cttctggcac
     2881 tggcgcttcg tcgaccaccg ggtcaacccc cgggcgctcg acttcgcgga gctcgggcgc
     2941 tcgtcgtggt tcggcaactt cgccgacggg gagttccgac tcgtcgacgg ggatctcgcg
     3001 gtcggcgacc ggtcgatctc cgacgccgac ccgaacatcg tggccggcca cgcgagcgcg
     3061 gctgccgagc ggcacaccgc cttcaactgg ctacgcggcg gccgctccta cggcgatacc
     3121 caggccaaca cctgacgatg tcgccgatga gttcggcgcc gccgcctcgt cgtacccgac
     3181 agcggccacc cggctgcgcg ccgcaccgag gagcccgcat gaccccgatc gacctcgccc
     3241 ccgccggcct gcggatggca gccctggtcg ccgccgttcc cgaggagctg ctcggagcgc
     3301 cgacgccgtg ccccgcctac tgcgtcgggg atctgctcga ccacatcgcg ggcctgggca
     3361 ccgcgttctc cgccgcggcg cggaaggacg ccggtgccga cagcgagcct gcaccgcccg
     3421 gcgaccggtc ccggctctcc gacgactggc gcagcgacat cgccgccggc ctgtcggagc
     3481 tcgccgacgc gtggcggcca ccagcggcgt ggagcgggat gacacgcgtc ggcggcgtcg
     3541 tcctccccgg cgaggtctgt gggctcgtcg gcctcaacga gctggtcgtc cacgcgtggg
     3601 acctcgggcg ggccaccggc cagccggtca acgccgagcc ggaggtgatc cgggcgctgc
     3661 tgccgatgct cgatcagttc tcgccgccga tcgacgcacc ccgcggcgac gggccgttcg
     3721 gaccccggtt cccggtgtcg tcgggcgctc ccctcctcga tcagctcgtg ggcctggccg
     3781 gacgtgaccc gtcatggtca
//
LOCUS       DAOSOA010000321         3787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24282_length_3787_cov_4.554126, whole genome shotgun sequence.
ACCESSION   DAOSOA010000321 DAOSOA010000000
VERSION     DAOSOA010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24282_length_3787_cov_4.554126"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..3473
                     /locus_tag="PKY13_14930"
     CDS             267..3473
                     /locus_tag="PKY13_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT81287.1"
                     /translation="MSRPSIRVSRGRRSDRLDDSLAAVLDATVGALDAAIIVADGEGR
                     VDLWNGGAEELYGWSAEEAVGLFARDLIVPAEDRDLASAVRASLAHDGEWQGWFRCRR
                     RDGSIIVVKVVNIVVPADDGSVRAMVSVARDLTDELAADRGAKALQALADASPDAVLT
                     VAGETIMSWSPAAQRLLGWSADEAVGQSVYLVIPPEHAEECSAVVAMVARGEAISGYE
                     TDRVAKDGSLVPVSLDVAPVFDAARHEWLGVVTMRDCRNRDVVIEAALTAEARLRSMM
                     TNSEEVVVVVGADRTIRAVGPSGAGLIGGTSTQLIGSSVVRFVHRDDRGAVDAALDQV
                     LADPAGTAQVLYRCPDRGGEWRWVEATMTNLMHDPAITGVLVNVVDVHEREQARSRLV
                     ERETLYRSLFESAADVAMVVDPSGVIREITPSCSRVLGKQPSSLVGRSLASAVSVELG
                     EMLDRNATPNDRELLTMRLALDEHDVIWIDAEVRNLAGESDLDGYVVNLRDVTARMIA
                     AQELHASQSRLSAIVNRSSDVAMFFAADGTICWVSPAVSEVFGVGPEALVGSKGPDLI
                     HEDDRDHVIATFAGGLGSIGDHVIVEFRVPDPNGGVLWLEGVATDLLDDPEVGYIVAN
                     VRDITDRKEADAELARLALIDEMTGLPNRLALLELIREGLAERAGDRSTSCGVVFFDI
                     DDFCDVNDSLGHGIGDALLVAMADRLAALMPEGCTLARFGDDQFAVFCRDVDTDAAMA
                     VACLVQRGLEEPFLVDVHEVFVVVSVGVALTPPGDVDSLLRRADTALYRAKKSGRGQT
                     VFFERELATESRKRLHYAGELRRGIERREIRPFYQPVVDLASGRVVAVEALARWHHPV
                     DGFIPPDVFIPAAEATGLIAELGRQILEQSCHDATMWADAGRPLQIAVNASAVQLMDT
                     TFPAQVAAALAGSGLPPEQLTFEITETAAMREMTVVLETLGRLRGIGCDLSLDDFGTG
                     YSSLSLLKRLPVQALKIDRSFVMGLGRSDDDERIVTGVVSLALVLGFRVVAEGVETSA
                     QMNALRSLGCQLGQGYLWSPAVPAHDLLATIAGIERFA"
     gene            complement(3480..>3787)
                     /locus_tag="PKY13_14935"
     CDS             complement(3480..>3787)
                     /locus_tag="PKY13_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81288.1"
                     /translation="QYPPQPGYPAQQPAAQQPGYAPPPPPQAYPQQPGYPPQQAYPQQ
                     GYAAGGYQPAQGYSPQGFAPGGGQRRQAGKSSNKLIMIVLGVVVRRTAARRTHGGEG"
BASE COUNT          507 a         1322 c         1328 g          630 t
ORIGIN      
        1 ccagtcgacc gcctcgagct cctccatcat cgacacgatc gcgcggtggc ggtcgatgac
       61 gtcgggcggg aggccctctt cgtggagcgt ggtgctgccg gcggtcatgt gcgcctcctg
      121 gtcggtcgtg gacggacgat ggggatgtcc gactccgtcg tggtcatcgc cccactctcc
      181 cacggcgccg gactcagggc gatgtcacct ttgcgcctct gggtgatctt cctcagaatc
      241 tcaagcatct ggccgatctc actcgtatga gccgtccgtc gatcagggtg tcacgcggtc
      301 gtcggagcga tcggctcgac gactcgctcg ccgccgtgct cgacgcgact gtcggagcgc
      361 tcgatgcagc gatcatcgtc gccgacggcg agggccgcgt cgatctgtgg aacggcggcg
      421 ccgaggagct ctacggctgg tccgccgagg aggcggtggg gttgttcgcc cgggacctca
      481 tcgtgccggc cgaggaccgt gacctcgcca gcgccgtgcg ggcatcgctc gcgcacgacg
      541 gtgagtggca ggggtggttc cggtgtcgtc gccgcgacgg ttcgatcatc gtcgtgaagg
      601 tggtcaacat cgtcgtgccg gccgacgacg gctcggtccg cgccatggtg agcgtcgctc
      661 gcgacctgac cgacgagctc gccgccgacc ggggtgccaa ggccctccag gccctcgccg
      721 acgcatcgcc cgacgctgtc ctcaccgtcg ccggcgaaac catcatgtcg tggagcccgg
      781 ccgcccaacg gctgctgggg tggagcgccg atgaggccgt cgggcagagc gtgtacctcg
      841 tgatcccgcc cgaacacgcc gaggagtgct ctgcggtggt cgcgatggtc gcccgtggcg
      901 aggccatctc gggctacgag accgaccgtg tggccaagga cggatcgctc gtcccggtgt
      961 cgctcgacgt cgcccccgtg ttcgacgccg cccgccacga gtggctcggt gtcgtcacca
     1021 tgcgggactg ccggaaccgc gacgtggtga tcgaggcggc gctgaccgcc gaggcgcgcc
     1081 tgcgttcgat gatgacgaac tccgaggagg tcgtggtcgt cgtcggtgcc gatcgcacca
     1141 tccgcgctgt cggtccatcc ggggccggcc tcatcggggg cacgtcgacg caactgatcg
     1201 gatcgtccgt cgtccgtttc gtgcaccgcg acgatcgcgg agcggtcgac gcggcgctcg
     1261 atcaggtcct cgccgacccc gccggtaccg cccaggtgct ctaccggtgc cccgaccgtg
     1321 gtggcgagtg gcggtgggtc gaggccacga tgacgaatct gatgcacgac ccggcgatca
     1381 ccggggtact cgtcaacgtc gtcgacgtcc acgaacgcga gcaggcgcgg tcccgcctcg
     1441 tggaacgcga gaccctctac cgctccttgt tcgagagcgc cgccgacgtg gcgatggtcg
     1501 tcgatccgtc cggcgtcatt cgcgagatca caccgtcgtg cagccgggtg ctcggcaagc
     1561 agccgtcgtc gctggtcggc cggtccctgg cgagcgccgt ttccgtggag ctgggcgaga
     1621 tgctcgatcg caacgccacg ccgaacgacc gcgaactcct cacgatgcgc ctcgcgctcg
     1681 acgagcacga cgtcatctgg atcgacgccg aggtccgcaa cctggccggt gaatccgacc
     1741 tcgacggcta cgtcgtcaac ctgcgcgacg tcaccgcccg catgatcgct gcccaggagc
     1801 tgcacgcctc ccagagccgg ctctctgcca tcgtcaaccg ttcgagcgac gtcgcgatgt
     1861 tcttcgcggc cgatggcacg atctgttggg tcagcccggc ggtgtccgag gtcttcggcg
     1921 tcgggcccga ggcgctcgtc gggtcgaagg gaccggacct catccacgag gacgaccgcg
     1981 accacgtgat cgccaccttc gccggcggcc tcgggtcgat cggtgaccac gtgatcgtcg
     2041 agttccgcgt ccccgacccc aacggtgggg tcctctggct cgagggggtc gcgaccgacc
     2101 tgctcgacga ccccgaggtc gggtacatcg tcgcgaacgt ccgcgacatc accgaccgca
     2161 aggaggccga cgccgagctg gctcgactcg cgttgatcga cgagatgacc ggcctcccga
     2221 accgcctcgc cctgctcgaa ctgatccgtg aggggctggc ggaacgggcc ggcgaccgct
     2281 cgacctcgtg cggtgtggtc ttcttcgaca tcgacgactt ctgcgacgtc aacgactcgc
     2341 tcgggcacgg catcggcgat gcgctcctcg tggcgatggc ggaccgcctc gcagcgctca
     2401 tgccggaggg gtgcaccctc gcccgcttcg gcgacgacca gttcgccgtg ttctgtcgcg
     2461 acgtcgacac cgacgcggcc atggcggtgg catgcctggt gcaacggggg ctcgaagagc
     2521 cgttcctcgt cgacgtgcac gaggtgttcg tcgtcgtcag tgtcggtgtg gcgctgaccc
     2581 cgcccggcga cgtcgactcg ctcctccgcc gcgccgacac cgcgctgtat cgggccaaga
     2641 agtcggggcg gggccagacc gtcttcttcg aacgggagct cgccaccgag tcgcgcaagc
     2701 gcctgcacta cgccggagag ctgcgacgtg gcatcgaacg ccgcgagatc cggccgttct
     2761 accagccggt ggtcgacctc gcgtcgggac gggtcgttgc ggtcgaggcg ctggcccgat
     2821 ggcaccaccc cgtcgacggg ttcattcctc ccgacgtgtt catccccgcc gccgaggcga
     2881 ccggactcat cgccgagctg ggccgccaga tcctcgaaca gtcgtgccac gacgccacca
     2941 tgtgggccga cgccggccga cccctgcaga tcgcggtcaa tgcctcggcc gtccagttga
     3001 tggacacgac gttccccgcc caggtcgccg ccgccctcgc cggatcgggg ttgccgcccg
     3061 agcagctcac cttcgagatc accgagacgg cggcgatgcg cgagatgacg gtggtgctcg
     3121 agacgctcgg ccgtctgcgc ggaatcgggt gtgatctgtc gctcgacgat ttcggcaccg
     3181 ggtactcgtc gctgtcgctc ctcaagcggt tgccggtgca ggccctgaag atcgaccgga
     3241 gtttcgtgat gggcctcggt cgttccgacg atgacgaacg gatcgtcacc ggtgtggtca
     3301 gcctggcgct ggtcctcggg ttccgggtgg tcgccgaggg ggtcgagacg agcgcccaga
     3361 tgaacgcgct gcgctcgctc ggctgccagt tggggcaggg gtacctgtgg tcaccggcgg
     3421 tgccggccca cgacctgctc gccaccatcg caggcatcga gcggttcgca tagcgaccct
     3481 cacccttcgc cgccgtgagt gcgccgcgct gcggttcgcc ggacgacgac cccgagcacg
     3541 atcatgatca gcttgttgga ggacttcccg gcctgccggc gctggccgcc gcccggggcg
     3601 aagccctgcg gcgagtagcc ctgcgccggc tggtagccgc cggccgcgta cccctgttgc
     3661 gggtaggcct gctgcggcgg gtagcccggc tgttgcgggt aggcctgcgg cggtggcggc
     3721 ggtgcgtagc cgggctgctg cgccgcgggc tgctgggcgg ggtagcccgg ctgcggcggg
     3781 tactgct
//
LOCUS       DAOSOA010000322         3774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24381_length_3774_cov_4.221027, whole genome shotgun sequence.
ACCESSION   DAOSOA010000322 DAOSOA010000000
VERSION     DAOSOA010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24381_length_3774_cov_4.221027"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PKY13_14940"
     CDS             <1..936
                     /locus_tag="PKY13_14940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT81289.1"
                     /translation="LAIDLMSRNAAIERVLPVVARYAERTGDGQRCQVIWWPEGQTRI
                     EVAPDSAPLPGPPATMVETTRSTREAQQLSRSSATPEQAAELFDEYAVLWTAPVLAPE
                     GRDVLGVMAIWGEVEFDLVLKQADHPAIRLATLALVDHRYKSMLRWDASHDSLTRVHN
                     RSGFAAALGATDGPCALLYLDLDDFKPVNDRFGHESGDRVLVRAAERISRAVREIDVV
                     ARLGGDEFAVICPGVDPDQAVEIARRISRAVARPVRIGGEPVKVGVSVGIAAAERPDE
                     RSRLLARADEALYRAKAEGKRRIAVAPAVGDDLDR"
     gene            947..1699
                     /gene="map"
                     /locus_tag="PKY13_14945"
     CDS             947..1699
                     /gene="map"
                     /locus_tag="PKY13_14945"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT81290.1"
                     /translation="MTIDNDDDLAGLERSGAVVAEARDAMVAAVVPGISTGELDAIGR
                     SVMRRHGARSAPRLAYRFPGSTCISINDEAAHGIPSTTRRLREGDLVNIDVSAELDGY
                     WTDTGISVAVGSVNPTAQRLLEATKAAQRDAMRAARAGRRISELGRAVEKRARSAGFS
                     VVENLCGHGVGRHIHEEPSIPSVEQRGDRTKLWEGLVIAIEPFLSTGATFVVEDPDGW
                     TLRTPDGSLTAQFEHTVVVTRGEPLVLTASAA"
     gene            1745..2341
                     /locus_tag="PKY13_14950"
     CDS             1745..2341
                     /locus_tag="PKY13_14950"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT81291.1"
                     /translation="MNDADPELIARIAGGDDQAFETFYRRHIDAVVANAVRRCRDAHE
                     VSELCAAVFLAVWQRAATFDAERGEAGAWLHGIAVHRLIDLRRGDRRRNALAARLIER
                     RVLDGDDIERLTERIDAERDADTVIDAIRALPGPQRDALALVAVDGLTATEAAERLGA
                     SPTAVRMRLSRARRTVRGRLGDVADPTNTTELPPEVAK"
     gene            2338..3612
                     /locus_tag="PKY13_14955"
     CDS             2338..3612
                     /locus_tag="PKY13_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81292.1"
                     /translation="MNDTFEDRLWSVLIDEVRAPSLPSFDEPSPLGRPRPVRRRTPLL
                     LAGGIAAAAALLAGVLVLGGDDGVSTVETADTAPTTTAAPDLPYLTLADPVGGVVQRV
                     SLPRDDQRRYRVRRIGAAPGVADIELTKSRGFMTTSGFNTVATIGGIEVRELDPSNSG
                     GVPMDLYHFDTPDGSSWVVSGLTSGADGLAELRAVLEAATFSGGGMSVAPPFEQFTTP
                     FNAPKAWSVQDNLVAVIGFHDSTSDIGDLPGAAVTVRGHEGREQDNMLTWRETPDLVV
                     SVVSSMQSMTATGVPPTPLADRVAAAERLVPGTRALYEALPVWRGASDSNVVVLQAPG
                     RQVGMVDFWVVDADGKQISSGGGSLGDAGSALPMVPGAAEVWLWNGDDVPGTSAGSDA
                     IRTAPPACAVHVVVPPAGQPPVEVTIDPDCTG"
BASE COUNT          552 a         1293 c         1374 g          555 t
ORIGIN      
        1 ctggcgatcg acctgatgag tcggaacgcc gcgatcgagc gggtactccc ggtggtcgct
       61 cggtacgccg aacgtaccgg cgacgggcag cgttgccagg tgatctggtg gcccgagggt
      121 cagacccgga tcgaggtcgc gcccgactca gcgccgctcc cggggccgcc ggcgacgatg
      181 gtggagacga cgcggtcgac gagggaggcg cagcaactga gccgcagcag cgcgacgccc
      241 gaacaggctg cggagctgtt cgacgagtac gcggtgctgt ggacggcgcc ggtccttgca
      301 ccggaggggc gcgacgtcct cggcgtcatg gcgatctggg gcgaggtcga gttcgacctc
      361 gtgctgaagc aggcggacca tcccgcgatc cggctggcga ccctggccct cgtggatcat
      421 cggtacaagt cgatgctgcg ttgggacgcg agccacgatt cgctcacgcg ggtccacaac
      481 cggtcaggat tcgcggccgc cctcggtgcg accgacgggc catgcgcgct gctctatctc
      541 gacctcgacg acttcaagcc ggtcaacgac cggttcggtc acgagagcgg cgaccgggtg
      601 ctcgtgcgcg ccgccgagcg catcagtcgc gccgtccgcg agatcgatgt cgtcgcccgg
      661 ctcggcggcg acgagttcgc cgtcatctgc cccggtgtgg acccggacca ggccgtggag
      721 atcgcccgcc gtatctcgcg ggcggtcgcg cgacccgtcc gcatcggtgg cgagccggtg
      781 aaggtcgggg tcagcgtcgg cattgcggct gcggaacgtc ccgacgagcg atcccgactc
      841 ctcgctcgtg ccgacgaggc gctctaccgc gccaaggccg agggcaagcg ccgcattgcg
      901 gttgcgccag ccgtggggga tgacctcgac cggtagcctc gccaccatga cgatcgacaa
      961 cgacgacgac ctcgcaggcc tcgagcgcag cggggcggtg gttgccgagg cccgtgacgc
     1021 gatggtcgcg gccgttgtgc ccggcatcag cacgggggag ctcgatgcga tcggtcgatc
     1081 ggtcatgcgc cgccacggcg cccggtcggc gcctcgactc gcgtaccggt tccccgggtc
     1141 gacgtgcatc agcatcaacg acgaggccgc gcacggcatc ccgtcgacga cgcggcgtct
     1201 tcgcgagggc gacctggtca acatcgatgt gtccgctgag ctcgacggct actggaccga
     1261 cacgggcatc agcgtcgcgg tgggatcggt gaacccgacc gcccagcggt tgctcgaagc
     1321 gacgaaagcg gcgcagcgcg atgcgatgcg tgcagcgcgg gcgggacgcc gaatcagcga
     1381 gctcggtcgc gcggtcgaga agcgggcccg atcggctggg ttctcggtcg tcgagaactt
     1441 gtgcgggcac ggcgtcgggc ggcacatcca cgaggagccg tccatcccga gcgtcgagca
     1501 acgcggcgac cgcaccaagc tgtgggaagg gctcgtgatc gccatcgaac cgttcctgtc
     1561 gacgggtgcg acgtttgtgg tcgaggaccc tgacggctgg acgctccgca cgcccgacgg
     1621 cagcctgacg gcgcagttcg agcacaccgt ggtggtcacc cgcggcgagc cgttggtact
     1681 caccgcatcg gcggcctgac agacctcggg ttacacgtcg ccccgacgcg acgaaggggt
     1741 tgatgtgaac gacgccgacc ccgaactcat cgcccggatc gcgggtggtg acgatcaggc
     1801 cttcgagacc ttctaccgtc ggcacatcga cgcggtggtc gccaacgccg tccgtcggtg
     1861 ccgggacgcc catgaggtgt ccgagctgtg cgccgcggtg ttcctcgcgg tgtggcaacg
     1921 cgctgcgacg ttcgacgccg agcgcggcga ggccggggcg tggcttcacg gcatcgctgt
     1981 gcaccggttg atcgatctcc gtcgcggtga ccgccgccgg aacgcactcg cggcccggtt
     2041 gatcgagcgc cgggtgctcg acggtgacga catcgaacgg ctcaccgaac gcatcgacgc
     2101 cgaacgcgac gcggacaccg tgatcgacgc gatccgagct ctccccggac cccaacgcga
     2161 cgccctcgcg ctggtcgcgg tggacggcct cacggcgacc gaggctgcgg agcgactcgg
     2221 cgcttcaccg accgcggtgc ggatgcgtct ctcacgggcg cggcgcacgg tgcgcggtcg
     2281 cctgggcgac gttgccgatc cgacgaacac gaccgagctc ccaccggagg tagcgaaatg
     2341 aacgacacgt tcgaggaccg cctgtggtcc gtcctgatcg acgaggtacg ggcgccgtcg
     2401 ctcccgtcct tcgacgagcc gagcccactc ggtcgtccga ggccggtccg ccgccgcacg
     2461 ccgctgctcc tggcgggtgg gatcgctgcc gctgccgcgc tgctcgccgg agtcctcgtg
     2521 ctcggagggg atgacggtgt gtcgacggtc gagaccgccg acacggcacc caccaccacg
     2581 gcggcgcccg acctgcccta cctcacgctg gccgatccgg tgggcggtgt cgtgcagcgg
     2641 gtcagcctgc cgcgcgacga ccaacggcgg taccgcgtcc gtcgcatcgg ggctgctccg
     2701 ggagtggcgg acatcgagct gacgaagtcg cgcgggttca tgacgacgag cgggttcaac
     2761 acggttgcga cgatcggcgg catcgaggtc cgggaactcg atccgtcgaa ctccggcggg
     2821 gtcccgatgg acctgtacca cttcgacacg cccgacggct cgtcgtgggt cgtgtcgggc
     2881 ctgacgtccg gcgccgacgg cctggccgag ctccgcgcgg tcctcgaggc ggcgacgttc
     2941 tcgggtggtg ggatgtcggt cgcgccgccg ttcgagcagt tcaccacgcc gttcaacgcc
     3001 ccgaaggcgt ggtcggtgca ggacaacttg gtggcggtca tcgggttcca cgactcgacg
     3061 tcggacatcg gcgaccttcc cggagcggcc gtgaccgttc ggggtcacga gggccgcgag
     3121 caggacaaca tgctgacgtg gcgggagaca cctgatctcg tcgtgtcggt cgtgagctcg
     3181 atgcagtcga tgaccgcgac gggtgtcccc ccgaccccgc tcgccgatcg tgtggcggcc
     3241 gccgagcggc tcgtccccgg gacccgggcg ttgtacgagg cgctaccggt gtggcgcggc
     3301 gcgtcggact cgaacgtggt cgtgctccag gcgccgggtc gacaggtcgg catggtcgac
     3361 ttctgggtcg tcgacgccga cggcaagcag atcagtagcg gtggagggtc gctcggcgac
     3421 gccggctccg ccctcccaat ggtcccgggt gcggcggagg tgtggctctg gaacggcgac
     3481 gatgtccccg gaacgtcggc cgggagcgac gcgatccgca ccgcaccgcc ggcgtgtgcg
     3541 gtgcatgtcg tcgttcctcc cgccgggcag ccgcccgtcg aggtcaccat cgatcccgat
     3601 tgcaccggat gacccgagtg cgtcagtcgg cctcggaggg atccggccgc tgtccgacga
     3661 gcaccgcgaa cgccagtccg gcgatggcgc cgaacaggtg gccttcccac gacaccccgc
     3721 ttcggggcac gaatccgaag atcagcccgc cgtagacgac gacggcgatg atcg
//
LOCUS       DAOSOA010000323         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24501_length_3759_cov_3.482721, whole genome shotgun sequence.
ACCESSION   DAOSOA010000323 DAOSOA010000000
VERSION     DAOSOA010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24501_length_3759_cov_3.482721"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2304
                     /locus_tag="PKY13_14960"
     CDS             <1..2304
                     /locus_tag="PKY13_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT81293.1"
                     /translation="EPHRGHLAYGAVAPVMRDLVGIDRELPPRDAGTALWEALPPAQR
                     PWAPLIADVLGAELPPTPEVGRIRPDHRRDKAISVLAELLSSAAPDQPARALFVEDAH
                     WLDESSAQVVAMLQRNGGPLGLSFVVTSRSSDVGGVAPTAVVALQPLPPHEAERLLHA
                     AIGDRAFSDAALASMQHRSDGNPLFLRELARSGATGRDLPETVEQVLAARIDVRPVDE
                     RRLIRELSVLGNTIDPHLAALVLERQDLEGPGGWRAISAFVVPAGDRMRFQHDLVRVA
                     AYEGLSFRRRRHLHERVAVLLEETAAPTPDPGETAWHWSRADRPDRTWLWARRAAEAA
                     TAKGAMGDAAQFLRDALDAGRRLDVDAAELAATHVALAEALEPVGELDDALAQLSRVD
                     RLVPTDLVTRAKVAHQRAHLLEFAGRYRDALGATTRGERLANEAGDEAAVVRLLLMRA
                     NLWACQGRFREARRLVDALGPRIERIGDVRLAAQAHLLMGIARDGDDDRDHEEQALTL
                     LRQLDDPVGLGNLLLNRGFSQFLANRWPDADASWTESAVQYGRAGDVVGGALTDVARA
                     EMLTYQHRLEEAERLLHRSRRILRSAGYPLGVALTASGLSRIELYRGRPGPARELLDE
                     AMTVFEQLGAEDYVLDTRLRIAEWELLAGSPAAALVILDDTSPAISRIGDPGILPVTE
                     ARLRGFVLLAADRAADARRAFRRAHTIASRDSFEFEMAMADLGLSACGDEFEHHRVGA
                     EALLRHLGVKAPPPTTGPITPPGRSPG"
     gene            complement(2276..2518)
                     /locus_tag="PKY13_14965"
     CDS             complement(2276..2518)
                     /locus_tag="PKY13_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81294.1"
                     /translation="MNENTNDDIRIEDTEAHAIRIRGNEPAQDAEGHAARVKGIAPAD
                     DAEGHATRGKGNTPADDAEGHAIKGRGITPANDPGE"
     gene            complement(2655..>3759)
                     /locus_tag="PKY13_14970"
     CDS             complement(2655..>3759)
                     /locus_tag="PKY13_14970"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT81295.1"
                     /translation="PVGGTVVGEDEPPARFRDGVAAVRASRPAMLLLSIFVAAVFSYG
                     FSIVLFVLVAVERLGLSDAGVGYLRMAEGVGGVAAAAIAGRVAAKNSVGALVGTALAA
                     GLTPVLLALTTSPAVAMVVLGLGGGAYVVFEVIVVSWLQRITPDEMLGRVMGLLMSMG
                     ALGTAFGAFLAPVLNGAIGLEWTLVVSGAVPIGLIIALWPMLPSIARDAAERQAELAP
                     IVDVLRGLSIFAAADDLTLEHLAGLVEPSEVAAGCVLMSEGDVADDFYVVRAGILDVR
                     SSGGSGGEATFVNAMGPDDWFGEIGLLQRSPRTATVTTTTAATLWRIPGDEFLAAFAG
                     SASRPDILGGTMAVRLARTHPGRAADIIDDIVT"
BASE COUNT          569 a         1374 c         1238 g          578 t
ORIGIN      
        1 gagccccacc gaggccatct cgcctacggc gccgtcgccc cggtcatgcg tgacctcgtc
       61 gggatcgacc gcgagctccc accccgcgat gccggaacgg cgctctggga ggccctaccg
      121 ccagcgcagc gcccgtgggc tccgctcatt gccgacgtgc tcggtgccga gcttcctccg
      181 acacccgagg tcggcaggat ccggcccgac catcgccgcg acaaggcgat ctccgtcctg
      241 gccgagctgt tgtcgagcgc cgctccggac cagcccgcgc gggctctgtt cgtcgaggac
      301 gcccattggc tcgacgagtc cagcgcccaa gtcgtcgcga tgctgcaacg caacggcggg
      361 ccactcggtt tgtcgttcgt ggtcacgtca cgcagcagcg acgtgggcgg cgtagcgccg
      421 acggccgtcg tcgccctgca acctctcccg ccgcacgagg ccgagaggct gctgcatgcc
      481 gccatcggcg accgtgcgtt cagcgacgcc gcgcttgcgt cgatgcagca ccgttccgac
      541 ggcaacccgc tgttcctgcg cgagctcgcc cgttcgggcg cgactggtcg ggaccttccc
      601 gagacagtcg agcaggtgtt ggcagcgcgc atcgacgtcc ggcccgtcga cgagcgacga
      661 ctgatccgag aactgtccgt gctcggcaac accatcgatc cccacctcgc cgcgctcgtg
      721 ctcgaacgcc aggatctcga gggccccggc ggctggcgcg ccatctccgc cttcgtcgtc
      781 cctgctggcg atcggatgcg attccagcac gacctcgtgc gagtcgccgc ctacgaggga
      841 ttgtcgttcc gccggcgacg ccacctccac gaacgcgtgg cggtgctgct cgaggagacg
      901 gctgccccca cccccgaccc cggcgagacg gcgtggcact ggtcgcgggc agatcgtccc
      961 gatcggacgt ggctgtgggc gcggcgggcc gctgaagcag ccaccgccaa gggcgccatg
     1021 ggcgacgcgg cgcagttcct gcgcgacgcg ctggacgccg gacgccgcct ggacgtcgat
     1081 gccgccgaac tggcggccac ccacgtggcg ctggccgagg cgttggagcc agtcggcgag
     1141 ttggacgacg cactcgcaca gttgtcccgg gtcgaccgcc tcgtcccgac cgacctcgtg
     1201 acccgcgcca aggtcgcgca ccagcgggcg catcttctcg agttcgccgg ccgctaccgg
     1261 gatgcgctgg gcgcgacgac ccggggtgaa cgcttggcga acgaggccgg ggacgaggcg
     1321 gcggtcgtcc gccttctgct gatgcgcgcc aacctgtggg cctgccaagg acgctttcgg
     1381 gaggcacggc gtctggtcga cgctctcggc ccacggatcg aacgcatcgg cgatgtacgc
     1441 ctcgcggcgc aggcgcacct cctgatgggg atcgcccgcg atggcgacga tgatcgcgac
     1501 cacgaggaac aggcgctcac gctgctccgg caactcgacg acccggtcgg tctcgggaac
     1561 ctgctcctga atcgtgggtt cagccagttc ctcgccaacc gctggcccga tgccgatgca
     1621 tcgtggaccg agagtgcggt ccagtacggc cgagccggcg acgtggtcgg cggagcgctg
     1681 accgacgtcg cccgggccga gatgctcacc taccagcacc gcctcgagga ggccgagcgc
     1741 ctgctccacc ggtcccgccg aattctgcga tcagcgggct atccgcttgg cgtcgcgctc
     1801 acggccagcg gcctcagccg catcgagctc taccgtggtc ggcccggccc ggcccgagaa
     1861 ctgcttgacg aagcgatgac ggtcttcgaa caactgggtg ccgaggacta cgtcctcgac
     1921 acccgtctcc ggatcgccga atgggagctc ctcgccggat cacctgctgc cgcgctggtc
     1981 atcctcgacg acacgagtcc ggcgatctcc cggatcggcg accccggcat ccttcccgtg
     2041 accgaggcgc gactccgggg cttcgtcctg ctcgctgccg atcgggccgc cgatgcgcgt
     2101 cgggcgttcc ggcgggcgca caccatcgcg agtcgcgatt cgttcgagtt cgagatggcc
     2161 atggccgacc tcggcctctc ggcgtgtggt gacgagttcg aacatcaccg cgtcggtgcc
     2221 gaggccctcc tccgccacct cggcgtgaag gccccgccac cgacgacggg tccgatcact
     2281 cccccgggtc gttcgccggg gtgatgcccc tccccttgat ggcgtggccc tcggcgtcgt
     2341 cggccggggt attgcccttc ccacgagtgg cgtggccctc ggcgtcgtcc gccggagcga
     2401 tgcccttgac ccgagcggcg tggccctcgg cgtcttgggc cggctcgtta ccgcggatcc
     2461 ggatcgcatg cgcttcggtg tcctcgatgc ggatgtcgtc gttggtgttc tcgttcatgt
     2521 cggtgctccc tgctcggtgg cttctcgtaa tcgcctggtg tcatcctgcg ccgaagcggc
     2581 cgcctcgtct gtcgtccgct cgactgttgc tgggtgcgcc ggccgacact tcccccctcc
     2641 ggtcggtacc gatatcacgt gacgatgtca tcgatgatgt cggctgctcg ccccggatgg
     2701 gtgcgagcca accgcaccgc catggtgccg ccgaggatgt cgggacgtga cgcgcttccg
     2761 gcgaatgctg cgaggaactc gtcgcccggg attcgccaca atgttgccgc cgtggtcgtc
     2821 gttaccgtcg ccgtgcgtgg gctgcgctgc aacagaccga tctcaccgaa ccagtcgtcc
     2881 ggtcccatcg cgttgacgaa cgttgcttcg ccacccgatc cacctgagga tcgaacgtcg
     2941 aggatgccgg cgcgcaccac gtagaagtcg tcggcgacat cgccttcgct catcaaaacg
     3001 cacccggcgg cgacctcgga cggctcgacg agaccggcaa ggtgttcgag ggtgaggtca
     3061 tcggccgcgg cgaagatcga gagaccgcgc agcacgtcga cgatcggggc cagttcggcc
     3121 tggcgttcgg cggcgtcgcg agcgatcgac gggagcatcg gccacaaggc gatgatgaga
     3181 ccgatgggga cggcaccgct cacgaccagc gtccattcaa ggccgattgc gccgttcaac
     3241 accggagcaa ggaaggctcc gaacgcagtg ccgagtgcgc ccatcgacat gagtagaccc
     3301 atcacacgac ccagcatctc gtcaggggtg atgcgctgca gccaactcac aacgatgact
     3361 tcgaacacga cgtacgcgcc accgcccagc cccaacacca ccatcgccac agccggcgac
     3421 gtcgtcagcg cgagcaggac cggggtgagg cctgcggcca gagcagtccc gacgagcgcc
     3481 ccgaccgaat tcttcgcagc cacccggcct gcgatggctg cggcggcgac accgccgacg
     3541 ccctcggcca tccgtagata gccgacgccg gcatcggaca gaccaagccg ttcgacggcg
     3601 acgagcacga acagaacgat cgagaacccg tacgagaaga ccgctgcgac aaagatcgac
     3661 agcagcagca tcgccggtcg cgacgctcgc accgcagcga ctccatcacg gaacctcgct
     3721 ggcggctcgt cctcaccgac caccgtcccg cccacgggc
//
LOCUS       DAOSOA010000324         3754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24539_length_3754_cov_5.423358, whole genome shotgun sequence.
ACCESSION   DAOSOA010000324 DAOSOA010000000
VERSION     DAOSOA010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24539_length_3754_cov_5.423358"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="PKY13_14975"
     CDS             <1..1356
                     /locus_tag="PKY13_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOT81296.1"
                     /translation="GGVAVGDLLTAVNGQPIRDVIEYRVLTDDAEVSLSIDRGGLAID
                     IDIEKNDGEPLGIEVDSAVFDRVRTCDNHCEFCFIYQLPKGMRKSLYLKDDDYRLSFL
                     YGNFTTLTRFTEADLERVITEHLSPLYVSIHATDPLTRNEMLRNKRGAMSLRWLRALL
                     DHGIEVHGQIVVCPGLNDGDILEDTLCGVLDEYPELADVAVVPLGLSKFNREPRMRLH
                     TAEEAKAVVDLVDEWQEIFRQHLGHRMVYAADEYYLIAGKPFPEPGAYDGFSMHEDGI
                     GMARAFELEFTGATDEAIGVQTGFFNWVDGAPAEGYRAPRMPEGAEPGSVLLRPRPPK
                     ADAPIGILTGSYGKSVLAPLIASLGRDDVRLIEVEARFFGGNVGVAGLMVGEDLQRVL
                     SSEPAGHRYLLPDVCLSGGRFLDGLTPEDLPRPVDIIPTDGVALRRALGLAMTEIARN
                     D"
     gene            1349..2680
                     /gene="der"
                     /locus_tag="PKY13_14980"
     CDS             1349..2680
                     /gene="der"
                     /locus_tag="PKY13_14980"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT81297.1"
                     /translation="MTDIETFPELPTVAIVGRPNVGKSTLMNRIIGRREAIVQELPGV
                     TRDRKVVPANWNGRDFMLVDTGGWMSGGSALDKKVSKQSEKAISEADVVLFVVDANVG
                     VTDDDLEVARLMQRIDRPVMVIANKVDDTRHEANIWDLLSLGLGDPYPVSALHGRGTG
                     DFLDVVVSHFPPEIEEPEFDMDADEMPALFSVALVGRPNVGKSTLFNRLIGEDRSVVH
                     DMPGTTRDSVDTVVETEHGSIKFIDTAGMRRKSRIDDGTEYFSMVRALQSVDEADAAL
                     LVIDATEGITHQDQRLAERIDASGSPIVVLLNKWELMNEDQRNEVLYQMTQKLHFLGD
                     SAVLRISALTGRGVHRLVPALETAIDAYHRRVPTRKVNDVIRRAQQAQPGPHGVRVMY
                     ATQGATDPPTFTLFANKVVPPHYLRYLERMLREAFDLGATPIKMRVRKRSE"
     gene            2697..>3754
                     /locus_tag="PKY13_14985"
     CDS             2697..>3754
                     /locus_tag="PKY13_14985"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOT81298.1"
                     /translation="MIERWFLVATAAVALVGGLVWGRAAYVRYRTVRKIDASATDLLQ
                     IAGGAISHDLHTAGLYLAVALAVGLVALTKDATGWYFIGVIALPVVVSLWLARYVRRD
                     ARLTEQRMRVEQRALEMLAQTDTAPARWAERLAPVEMPATPGYEVGTVHEAVSGVMSG
                     DLVDVFHLPSGRLCCVVGDVSGHDVEASITALQTKYLLRSYLRRFRDPGQALEELNRQ
                     VSDYERPEEFVSLCVLIFDSEAGTLRYASAGHPPAWFSHERELKPLKATGPVLMMDPA
                     AKFLSREVSFEPGDIVVALSDGVAEARSGSQFFGDERIAAVVRRFDDAPAQTMCHELV
                     NAAQEFASGPVTDDITVLA"
BASE COUNT          608 a         1281 c         1260 g          605 t
ORIGIN      
        1 gggggtgtcg cggtcggaga tctgctcacc gccgtcaacg gccagccgat ccgtgacgtc
       61 atcgagtacc gcgtcctgac cgacgatgcc gaggtgagcc tgtcgatcga ccggggcggc
      121 ctggcgatcg acatcgacat cgagaagaac gacggtgaac cgctcggcat cgaggtcgac
      181 tcggcggtgt tcgaccgggt gcggacctgc gacaaccact gcgagttctg cttcatctac
      241 cagctgccca agggcatgcg gaagagcctg tacctgaagg acgacgacta tcgactctcg
      301 ttcctctacg gcaacttcac gacgctcacc cgcttcaccg aggccgatct ggaacgggtg
      361 atcaccgagc acctgtcgcc gttgtacgtg tcgatccacg cgaccgaccc gctcactcgc
      421 aatgagatgt tgcgcaacaa gcggggcgcc atgagcctgc ggtggttgcg ggcgctcctc
      481 gaccacggca tcgaggtcca cggccagatc gtggtgtgtc ccggcctcaa cgacggcgac
      541 atcctcgagg acacgctgtg tggggtgctc gacgagtatc ccgaactcgc cgacgtcgcc
      601 gtggtgcccc tggggttgtc gaagttcaac agggagcccc gtatgcggct ccacaccgcc
      661 gaggaggcga aggccgtcgt cgacctggtc gacgagtggc aggagatctt ccgccaacac
      721 ctcggccatc gcatggtcta cgccgccgac gagtactacc tcatcgccgg caagccgttc
      781 cccgagccgg gcgcgtacga cggattctcg atgcacgagg acggcatcgg catggcccgg
      841 gccttcgagc tcgagttcac cggtgcgacc gacgaggcca tcggcgtgca gacggggttc
      901 ttcaactggg tcgacggggc gcccgccgag ggctaccggg ccccccgaat gccggaggga
      961 gctgagccgg ggagcgtgct cctgaggccg cgcccgccca aggccgacgc accgatcggc
     1021 atcctgaccg ggtcgtacgg caagtcggtc ctcgccccac tgatcgcgag cctcgggcgc
     1081 gacgacgtac gtctgatcga ggtcgaggca cggttcttcg gtggcaacgt cggcgtcgcc
     1141 ggactcatgg tgggggagga cctccagcgg gtgctttcgt cggaaccggc cggtcaccgc
     1201 tatcttcttc ctgacgtgtg tctgtccggt ggccgatttc tcgacggcct cacccccgag
     1261 gaccttcccc gacccgtcga catcatcccc accgacggcg tcgcgctgcg gcgggcgtta
     1321 ggactcgcaa tgactgagat cgcacgcaat gactgacatc gaaacgttcc ccgaattgcc
     1381 caccgtggcg atcgtcggcc ggcccaacgt gggcaagtcc acgttgatga accgcatcat
     1441 cggtcgccgc gaggcgatcg tgcaggagct gcccggggtg acccgcgacc gcaaggtcgt
     1501 gccggccaac tggaacggcc gcgatttcat gctggtcgac accggcggct ggatgagtgg
     1561 tggttcggca ctcgacaaga aggtgtcgaa acagtccgag aaggccatct ccgaggccga
     1621 cgtcgtgctc ttcgtcgtcg acgccaacgt cggcgtcacc gacgacgacc tcgaggtcgc
     1681 ccgcctgatg cagcgcatcg accgaccggt gatggtcatc gccaacaagg tcgacgacac
     1741 ccgccacgag gccaacatct gggacctgct ctccctcggt ctcggcgatc cgtaccccgt
     1801 gagcgcgctc cacggccggg gcaccggcga cttcctcgac gtggtcgtca gccacttccc
     1861 gcccgagatc gaagagcccg agttcgacat ggacgccgac gagatgccgg cgctgttctc
     1921 ggtcgccctc gtcggtcgcc ccaacgtcgg caagtcgacg ctcttcaacc ggctcatcgg
     1981 cgaggaccgc tcggtggtcc acgacatgcc cggcacgacc cgcgactcgg tcgacacggt
     2041 cgtcgagacc gagcacgggt ccatcaagtt catcgacacg gccgggatgc gccgcaagag
     2101 ccgcatcgac gacggcaccg agtacttctc gatggtgcgg gcgttgcagt ccgtcgacga
     2161 ggccgatgcc gcgctcctcg tgatcgacgc gaccgagggc atcacccacc aggaccaacg
     2221 gctcgccgag cgcatcgacg cgtcgggcag cccgatcgtg gtgctcctca acaagtggga
     2281 gctgatgaac gaggaccagc gcaacgaagt cctctaccag atgacccaga agctgcactt
     2341 cctgggcgat tcggcggtac tgcgcatctc ggcgctcacc ggtcgcggtg tgcaccggct
     2401 ggtgcccgcg ctcgagacgg cgatcgatgc ctaccaccgt cgggttccca cccggaaggt
     2461 caacgacgtc atccgacggg cacagcaggc gcagccgggg ccgcacggtg tgcgggtcat
     2521 gtatgcgacg cagggtgcga ccgatccgcc cacgttcacg ctgttcgcca acaaggtggt
     2581 cccgccccac tacctccgct acctcgagcg gatgctccgc gaggcgttcg acctcggagc
     2641 gacccccatc aagatgcgcg tccggaagcg cagcgagtag agccggcgac agcgtcatga
     2701 tcgaacgctg gttcctcgtc gccaccgccg ccgttgcgtt ggtcggcggg ctcgtgtggg
     2761 gccgagccgc gtacgtgcga taccgaacgg tccgcaagat cgacgcgtcg gcgaccgacc
     2821 tgttgcagat cgccggggga gcgatctccc acgacctgca caccgccggg ttgtacctcg
     2881 ccgtggcgtt ggcggtgggg ctcgtcgcgc tcacgaagga cgccaccggc tggtacttca
     2941 tcggcgtgat cgccctgccg gtcgtcgtca gcctgtggct cgcccggtac gtacggcgcg
     3001 atgcccgcct caccgagcag cggatgcgcg tcgagcagcg tgccctcgag atgttggccc
     3061 agaccgacac ggcaccggct cgctgggccg agcgcctcgc ccccgtcgag atgcccgcga
     3121 cgccggggta cgaggtcggc acggtgcacg aggcggtgtc gggggtgatg tcgggcgatc
     3181 tggtcgacgt gttccacctg ccgtcgggcc gcttgtgctg tgtcgtcggc gacgtgtcgg
     3241 gtcacgatgt cgaggcgtcg atcacggcgc tgcagaccaa gtacctcctc cggtcgtacc
     3301 tgcgtcggtt ccgtgacccg ggccaggcgc tcgaggagct caatcgccag gtgtcggact
     3361 acgagcgccc cgaggagttc gtgtcgctct gtgtgctgat cttcgacagc gaggccggca
     3421 cgctgcgata cgcgtcggcc gggcacccgc cggcgtggtt cagccacgag cgcgagctca
     3481 agccgttgaa ggcgaccggt ccggtcctga tgatggatcc ggcggcgaag ttcctgtccc
     3541 gcgaggtctc gttcgagccg ggcgacatcg tcgtagcgct gtccgacggc gtggcggagg
     3601 cccgatcggg cagccagttc ttcggcgacg agcgaatcgc cgcggtggtg cgccggttcg
     3661 acgacgcgcc cgcgcagacg atgtgccacg agctggtgaa cgccgcccag gagttcgcgt
     3721 cgggcccggt gaccgacgac atcacggtgc tcgc
//
LOCUS       DAOSOA010000325         3727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24766_length_3727_cov_6.028867, whole genome shotgun sequence.
ACCESSION   DAOSOA010000325 DAOSOA010000000
VERSION     DAOSOA010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24766_length_3727_cov_6.028867"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKY13_14990"
     CDS             complement(<1..280)
                     /locus_tag="PKY13_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOT81299.1"
                     /translation="MTLVAAIVLILANGFFVAVEFALLASLRSRIEEESGKLRGRLAL
                     RSMGRLGTVLAGTQLGVTLASLALGSVAEPAADRFLVSVFHSVNIPESF"
     gene            complement(277..1581)
                     /locus_tag="PKY13_14995"
     CDS             complement(277..1581)
                     /locus_tag="PKY13_14995"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT81300.1"
                     /translation="MAAVLVLVAATAFFVAVEFSLVAVDRNEVEIAAGGGDKRAAMVS
                     ASLKRLSFHLSGVQLGITVCSVGIGVLAEPNVASLLRSPIEAIVGAANAETVSVLLAL
                     LIATVVQMVVGELVPKAIAVARPLGTARFLAPAERVYSGVFRPVIVLFGGTADALVRR
                     LGVEPTEELSQVRSRHELAKLVESSGAQGSLQPAEVELLTRTFRFRDKDAAEALTPRT
                     AVVALSVADCGADLRQRSAETGFSRFLLYGADLDDVVGLVHVKALFDIEPERRDTVPL
                     VELRTDVLVVPESRPLDGLLLEMRASRVYLAVVLDEYGGTAGIITLEDLLEEIVGEID
                     DEHDQATDRAPVWSWGGTSVLAGRLSLDEVYDSTKLRLPEGDYETLAGFVLDQLGHIP
                     EVGDGFTYENWSIDVLEMDRHRVASVRLVQTIRPDDEPEGRS"
     gene            1674..>2296
                     /locus_tag="PKY13_15000"
     CDS             1674..>2296
                     /locus_tag="PKY13_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT81301.1"
                     /translation="MPPITPTTHAALELTDVGLRRGDDQVLTDITWRVQRDERWVVLG
                     ANGCGKTSLIRIAALYDHPSTGSVRVLGELLGRTDVRSLRRRVGFVSSAFVDLVRRDL
                     IASDVVMCALNASLEPWWHQYTDDDRARAVASLDRLGIARLATHRFGTLSSGERQRVQ
                     LARALMTDPELLLLDEPTAGLDIAGREEFVADLDAIGADGTPLALVT"
     assembly_gap    2297..2396
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2397..2827)
                     /locus_tag="PKY13_15005"
     CDS             complement(<2397..2827)
                     /locus_tag="PKY13_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT81302.1"
                     /translation="MDITATLHTDVDAEVLFGVVADLGGYPRWLDIVGRAVEAPADAG
                     DPGPAWSVDLRGQLGPLRRSKRLRMVRTVADEPRHVRFERRELDGKQHSAWILEATID
                     PGDGVDADASTALAMRLHYGGSLWVPMLDRVLRDEIERSRPR"
     gene            complement(2827..>3727)
                     /locus_tag="PKY13_15010"
     CDS             complement(2827..>3727)
                     /locus_tag="PKY13_15010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT81303.1"
                     /translation="RFQELATRIDVVGVEREDKVASASAAKANLRRRAVAAFVRGDQA
                     TQLIAVMGDPLDYSRGQRYLEALADSDQRAYDDYRSRIAEMNELERDLVDDQADLQNE
                     VDALKDERAAALQVLLDARRCADAYAGGSHACPPSFTFPVLGEVTFADTWGAARLPGT
                     ADQHWHEGTDIMAPEGREIVAVENGTLFKVGDQGLGGLRLWLRGDSGIEYYYAHFSSF
                     APIAVDGAVVTAGTVVGYVGSTGDAAGGAPHLHFEVHPAGGSPVDPYPLLKATWGRRA
                     MPLQSTIMERVASVTGTLPPEER"
BASE COUNT          572 a         1274 c         1276 g          505 t
ORIGIN      
        1 cgaacgactc ggggatgttc accgagtgga agacgctcac gaggaaccgg tccgccgccg
       61 gctcggccac cgagccgagg gccagcgaag cgagcgtcac gccgagctgg gtgccggcga
      121 gcacagtgcc gagccggccc atcgaccgga gcgcgagccg cccgcgaagc tttcccgact
      181 cctcctcgat gcgactccgg agcgacgcca acagggcgaa ctcgaccgcc acgaagaacc
      241 cgttcgccag aatgagcacg atcgccgcga cgagcgtcac gagcgcccct cgggctcgtc
      301 gtcgggacgg atcgtctgca cgagtcgcac gctggcgacc cggtggcggt ccatctccag
      361 cacgtcgatc gaccagttct cgtaggtgaa tccgtcaccg acctcgggga tgtgcccgag
      421 ctggtcgagc acgaagcctg cgagcgtctc gtagtcgccc tcggggagcc ggagcttggt
      481 cgagtcgtac acctcgtcga ggctcagccg tccggcgagg accgacgtgc caccccagct
      541 ccacaccgga gcgcggtccg tggcctggtc gtgctcgtcg tcgatctcgc cgacgatctc
      601 ctcgagcagg tcctcgaggg tgatgatgcc ggcggtgccg ccgtattcgt cgagcaccac
      661 ggcgaggtac acccgcgagg cgcgcatctc gaggaggagg ccgtcgagcg gtcgcgactc
      721 ggggacgacg aggacgtcgg tgcggagctc gacgagcggg acggtgtcgc ggcgctccgg
      781 ttcgatgtcg aacagggcct tcacgtgcac gaggccgacg acgtcgtcga ggtcggcgcc
      841 gtagagcagg aagcgactga acccggtctc ggccgaccgc tgccgcagat cggcgccgca
      901 atcggcgacc gacagggcca cgacggccgt ccgcggcgtg agggcctcgg ccgcgtcctt
      961 gtcgcggaac cggaacgtgc gggtgagcag ctcgacctcg gccggctgga ggctcccctg
     1021 ggcgcccgac gactcgacga gcttcgccag ctcgtggcgc gaccggacct ggctcagttc
     1081 ctcggtgggc tccacaccga ggcggcgcac gagcgcgtcg gcggtcccgc cgaacaacac
     1141 gatgaccggc cggaacacac cggagtagac gcgctcggcg ggagcaagga agcgggcggt
     1201 gccgagcggg cgtgccaccg cgatcgcctt gggcacgagc tcacccacga ccatctggac
     1261 caccgtcgcg atgagcagcg ccaagaggac cgacacggtc tcggcgttcg ccgcgccgac
     1321 gatcgcctcg atcggtgagc ggagcaagga cgcgacgttg ggctcggcga gcacaccgat
     1381 gccgaccgaa cacaccgtga taccgagctg gacgcccgag aggtggaacg acagccgctt
     1441 gagcgacgcc gacaccatgg cggcgcgctt gtcaccacca ccggcggcga tctcgacctc
     1501 gttacggtcg acggccacca aggagaactc gaccgccacg aagaacgctg tggccgccac
     1561 caggacgagg acggccgcca accctaggaa cgcgctgatg gctgtgtccg ccttccggag
     1621 tgcatccgta gctgtggtcg tcgtgtggtg ccggccatgt caggatcacg ctaatgcctc
     1681 ccatcacgcc gacgacccat gctgccctcg agctcaccga cgtcggtctg cgccgcggcg
     1741 acgaccaggt actcaccgac atcacgtggc gcgtgcaacg cgacgaacga tgggtcgtgc
     1801 tcggcgccaa cgggtgcggc aagacctcgc tcatccgcat cgccgcgctc tacgaccacc
     1861 cctcgacggg ctcggtccgg gtcctcggcg agttgctcgg acgcaccgac gttcggtcac
     1921 ttcgtcgacg ggtgggattc gtcagctcgg cgttcgtgga cctggtgcga cgcgacctca
     1981 tcgcatccga cgtggtcatg tgcgcgctga acgcgtcact cgagccgtgg tggcaccagt
     2041 acaccgacga cgaccgcgcc cgtgccgtgg cgtcgctcga ccgtctcggc atcgcccgac
     2101 tggcaacaca ccgcttcggg accctgtcga gcggtgagcg gcagcgggtg cagctggccc
     2161 gagccctcat gaccgacccg gagctgctgc tgctcgacga gccgaccgcc ggcctcgaca
     2221 tcgccggcag agaggagttc gtggcggacc tcgacgcgat cggtgccgac ggcacgccgc
     2281 tggcgctcgt cacccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg
     2401 ggcgcgaccg ttcgatctcg tcgcggagca cgcggtcgag catcggcacc cacaacgaac
     2461 cgccgtagtg caggcgcatc gccagcgcgg tcgaggcatc ggcgtcgacg ccgtcacccg
     2521 ggtcgatcgt ggcctcgagg atccaagcgg aatgctgttt gccgtcgagc tcgcgccgtt
     2581 cgaaccgcac gtggcgaggc tcgtcggcga ccgtacgcac cattcgcagg cgcttcgaac
     2641 ggcgcaacgg acccagctga ccacggaggt cgaccgacca cgccgggccc gggtcaccgg
     2701 cgtcggcggg cgcctcgacg gcgcggccga cgatgtcgag ccaccggggg taaccgccga
     2761 gatcggcgac gacgccgaac aggacctccg cgtcgacgtc ggtgtgcagg gtcgcggtga
     2821 tgtccatcaa cgttcctcgg gaggcagggt gccggtgacg ctcgccaccc gctccatgat
     2881 cgtcgattgg aggggcatgg cccgacggcc ccacgtggct ttcagcaacg ggtacgggtc
     2941 gaccggcgat cccccggcgg ggtgcacctc gaagtggagg tggggcgccc caccggcggc
     3001 gtcgccggtg ctgccgacgt agccgacgac cgtgcccgcc gtgaccaccg cgccgtcgac
     3061 ggcgatggga gcgaacgaac tgaagtgggc gtagtagtac tcgatgccgc tgtcgccgcg
     3121 aagccagagg cggaggccac ccaatccctg gtcgccgacc ttgaacaagg tgccgttctc
     3181 gacggcgacg atctcccgac cttcgggcgc catgatgtcg gtgccctcgt gccagtgctg
     3241 gtcggccgtg ccggggagcc gggcggcccc ccacgtgtcg gcgaacgtca cctcgcccag
     3301 cacggggaac gtaaagctcg gcggacacgc gtggctgccg ccggcgtagg cgtcggcgca
     3361 tcgacgggcg tcgagcagga cctgcagagc ggcggcccgc tcgtccttca acgcgtcgac
     3421 ctcgttctgc aggtcggcct ggtcgtcgac gaggtcgcgt tcgagctcgt tcatctcggc
     3481 aatgcggctc cggtagtcgt cgtaggcgcg ctggtcggag tcggcgagcg cttcgaggta
     3541 ccgctggccc cggctgtagt cgagcgggtc gcccatcacg gcgatgagct gggtcgcctg
     3601 gtcaccgcgg acgaacgcgg cgacggcgcg gcgccggagg ttggccttcg ccgccgacgc
     3661 gctcgcgacc ttgtcctcgc gttcgacccc gaccacgtcg atccgggtcg cgagttcctg
     3721 gaaccgg
//
LOCUS       DAOSOA010000326         3723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24797_length_3723_cov_7.160305, whole genome shotgun sequence.
ACCESSION   DAOSOA010000326 DAOSOA010000000
VERSION     DAOSOA010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24797_length_3723_cov_7.160305"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1622
                     /locus_tag="PKY13_15015"
     CDS             171..1622
                     /locus_tag="PKY13_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT81304.1"
                     /translation="MRILVMGATGYIGGRLVPRLVGAGHDVRCLTRRPATLVGVVWAD
                     SVEIARGDALDRASLDEAMSGVDVVYYLVHSIGSGTHFEETDRLAATNTAAAAAAAGV
                     GRIVYLGGLVPEGETASPHLASRAEVGQILLDGPVPAVVLQAGVIIGSGSASFEMLRY
                     LTERLPIMVTPRWVRSLVQPIAVRDVLRYLVGSIELDPTTNRRFDIGGPDVVSYREMM
                     QRYATVAGLTHRVIIPVPVLTPRLSSHWVNVVTPVPKAIAQPLIESLRNNAVTHEHDI
                     EDLIPFALCPFDEAVRLALESVRNAEVVTRWSGAEWPGAPSDPMPTDPDWSGGTVYRD
                     DREVPLDATTDAVWRAVEGIGGDRGWYSFPLAWAVRGLLDRLAGGVGLRRGRRDPDRL
                     RVGEPVDFWRVEALERPHLLRLRAEMKLPGEAWLEFSIREDTGRPVLHQRAMFIPHGL
                     AGHIYWWSVAPFHGVVFGSMIRNLAASAEAESA"
     gene            complement(1672..2235)
                     /locus_tag="PKY13_15020"
     CDS             complement(1672..2235)
                     /locus_tag="PKY13_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81305.1"
                     /translation="MAVIDVPGFIADLKDHATRHGFHVHDERHFVETYSMRQAWEVDL
                     HPEEACGGPLDLHLSLEVDPRIVLAFEDLVIDLPEDDEPDSPLAFPLNFTWSMPPLPH
                     CPDLLVLATELAGIGGPDLPLEVSAIDSFASVTDPAERSLTVIARIDVALAQIFMDTE
                     DLCSALDGCLDVSRYLLDQSPIWLDDL"
     gene            2289..3032
                     /locus_tag="PKY13_15025"
     CDS             2289..3032
                     /locus_tag="PKY13_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HOT81306.1"
                     /translation="MDVTSTWALFDEVAAAVRHRLVSSDLVAAGSRAGQYQLDLDADA
                     VVVEMLTAAGYGVMSEESGRHHPDRAVCVVVDPVDGSTNASRGLPCWATSLCAVDADG
                     PWVATVVDQSRDVTYRAARGGGATRSVSGGAPVPIAVGSPVALSDAIVALNGWSDHRP
                     RTRQYRALGAAAIELCLVADGSFDGYVNLDGDSHGPWDYLGALLVLREAGGVAVDRQG
                     RDLVSLEHAARRCIVAGSDEALITAMMSQ"
     gene            3029..3523
                     /locus_tag="PKY13_15030"
     CDS             3029..3523
                     /locus_tag="PKY13_15030"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT81307.1"
                     /translation="MSSRSGGAQLAGLKLYGRMPPLMRQRFVRAVKPSFTVGTMSVVT
                     KPDGAILLVRHSYLANWSFPGGLLNRRELPSVGAARETREEVGIRIELAGEPAVVVDP
                     YKQVVRVIYRAALADGVGPDDAHPASAEIVEVGWFQPDAIAAMSPESADALAALVRCE
                     RRGR"
     gene            3634..3707
                     /locus_tag="PKY13_15035"
     tRNA            3634..3707
                     /locus_tag="PKY13_15035"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3668..3670,aa:Val,seq:tac)
BASE COUNT          517 a         1240 c         1351 g          615 t
ORIGIN      
        1 ccgcctcggc cccggaatcg atcgcccgtg tcgacatccc ccgaaaccta gttgcgctgt
       61 cggcgaggat ccgaaccatc gggacgtcgg gactctgggc aggtcgaccg gttcggtcct
      121 cggctcccat cagcttcgtc gattccggtt tcgggtcgta ggttgcggtg atgaggatcc
      181 tcgtcatggg cgctaccggg tacatcggcg gtcgcctcgt gccccgcctc gtcggggcgg
      241 gccacgacgt ccgatgcctc actcgccgac cggcgacgtt ggtcggcgtg gtgtgggcgg
      301 actcggtgga gatcgcccgc ggcgacgcgc tcgaccgcgc cagcctcgac gaggcgatga
      361 gcggtgtcga cgtcgtctac tacctcgtgc actcgatcgg gagcgggacg cacttcgagg
      421 agaccgaccg cctcgcggcg accaacaccg ctgcggcggc agcggcagcg ggggtcggtc
      481 gcatcgtgta cctgggcgga ctggtgccgg agggcgagac ggcgtcgccg cacctggcgt
      541 cgcgtgccga ggtgggtcag atcctgctcg acgggcccgt gcccgccgtc gtgttgcagg
      601 ccggcgtgat catcggcagc gggtcggcat cgttcgagat gctgcgctac ctgactgagc
      661 ggctcccgat catggtcacg ccccgttggg tgcgctcgct cgtgcaaccg atcgctgttc
      721 gcgacgtact ccgctacctg gtcggttcga tcgagctcga tccgaccacc aaccggcgct
      781 tcgacatcgg cgggcccgac gtcgtctcct accgcgagat gatgcagcgc tacgccacgg
      841 tcgccggcct gacccaccgg gtgatcatcc cggtcccggt cctgacgccg cggctctcga
      901 gccattgggt caacgtggtg acgcccgtcc cgaaggcgat cgcccagccg ctgatcgaat
      961 cgctacgcaa caacgccgtc acccacgaac acgacatcga ggacttgatc cccttcgcgt
     1021 tgtgcccgtt cgacgaggcg gtccgcctcg cactggagag cgtgcggaac gccgaggtcg
     1081 tcacccgctg gtcgggtgcg gagtggccgg gtgccccgag cgaccccatg ccgaccgatc
     1141 cggactggag cggcggcacc gtctaccgcg acgaccgcga ggtgcccctc gatgccacga
     1201 cggatgctgt gtggcgggcg gtcgagggaa tcggagggga ccgcgggtgg tactcgttcc
     1261 cactggcatg ggccgtgcgg ggcctgctcg atcggttggc cgggggtgtg gggctgcggc
     1321 gcgggcgacg cgatcccgat cggctccggg tcggcgagcc cgtcgacttc tggcgggtcg
     1381 aggcgttgga gcggccgcac ctgctccgcc tgcgcgccga gatgaagttg cccggcgagg
     1441 cgtggctcga gttctcgatc cgcgaggaca ccgggcgacc cgtgctgcac cagcgggcga
     1501 tgttcatccc gcacggcttg gcggggcaca tctactggtg gtcggtggcg ccgttccacg
     1561 gtgtggtgtt cggttcgatg atccggaacc tggcagcgtc ggcggaggcc gagtcggcct
     1621 aggtccgcag cggccgaagt ggaggcgtcg ctcgatctcg aggcggggtg ttcagaggtc
     1681 gtcgagccag atgggtgact ggtcgagcag gtaacggctg acgtcgaggc agccgtcgag
     1741 cgccgagcag agatcttcgg tgtccatgaa gatctgggcg agggcgacgt cgatgcgggc
     1801 gatgaccgtg aggctgcgct cggcagggtc ggtgaccgac gcgaacgagt cgatcgccga
     1861 cacctcgagg ggcagatcgg ggccaccgat gccggccagc tcggtggcga gcaccagcag
     1921 gtcgggacag tgggggagcg gcggcatcga ccacgtgaag ttgaggggaa acgccagcgg
     1981 ggagtcgggt tcgtcgtcct cgggaaggtc gatcacgagg tcttcgaacg ccagcacgat
     2041 acgggggtcg acctcgaggg agaggtgcag gtcgagcggg ccgccgcacg cctcctcggg
     2101 gtgcagatcg acctcccagg cctgccgcat cgagtacgtc tcgacgaaat gccgttcgtc
     2161 gtggacgtgg aaaccgtgcc gggttgcgtg atccttcagg tcggcgatga agccggggac
     2221 atcgatgact gccattcgca acagcctgcc atctcagcca taccggggcg agccagtacc
     2281 gtgtcgcggt ggacgtcacc tcgacctggg cgctgttcga cgaggtcgct gcggcggtgc
     2341 gccaccggct cgtgtcgtcc gacctcgtcg ccgccgggtc acgagccggt cagtaccagc
     2401 tcgacctcga cgccgacgcc gtcgtggtgg agatgctgac ggccgccgga tacggcgtga
     2461 tgtcggagga gtcaggccgc catcaccccg atcgggccgt gtgcgtcgtc gtcgatccgg
     2521 tcgacggttc gaccaacgcg tcccgtggac tcccgtgctg ggcgaccagc ctgtgcgccg
     2581 tcgacgccga cggtccgtgg gtcgcgaccg tcgtcgacca gtcccgcgac gtcacctatc
     2641 gcgctgctcg gggcgggggc gcgacgcgct cggtgagcgg tggcgcaccc gtgcccatcg
     2701 ccgttggttc gccggtcgcc ctctccgacg cgatcgtggc actcaacggc tggtcggatc
     2761 atcgtccccg cacccggcag taccgtgcgc tcggcgccgc ggcgatcgag ctgtgcttgg
     2821 tcgccgacgg ttcgttcgac gggtacgtca acctcgacgg cgactcccac gggccctggg
     2881 actacctcgg tgccctcctc gtgctgcgcg aggcgggggg tgtggcggtc gatcggcagg
     2941 gacgggatct tgtctcgctc gagcacgccg cccgacgctg catcgttgct ggctccgatg
     3001 aagcgctcat cactgcgatg atgtcgcaat gagttcgcgt tcgggcggag cacaactcgc
     3061 cggcctcaag ctatacggac ggatgccgcc gctcatgcgg cagcggttcg tgcgagccgt
     3121 caagccctcg ttcaccgtcg gcacgatgtc ggtggtcacc aaacccgacg gcgcgatcct
     3181 gctcgtgcgg cacagctacc tcgcgaactg gagcttcccc gggggcctgt tgaaccgtcg
     3241 ggagctgccg tcggtcggtg ccgcccgtga gacgcgcgag gaagtcggga tccgcatcga
     3301 gctggcgggc gaacccgccg tcgtcgtcga cccgtacaag caggtggtgc gggtgatcta
     3361 ccgggccgcc ctcgctgatg gtgtcggccc cgacgacgcg cacccggcat cggccgagat
     3421 cgtcgaggtc ggctggttcc agcccgacgc catcgcggcg atgtcgcccg agtcggccga
     3481 cgccctcgcc gcgctcgtcc gctgcgagcg ccgcggccgc tgaccgcacc cgttctgtcc
     3541 cgcgaaggga tccagaacgc atcccttcgc gggacagaac gtcttcgctg gcgagcgggc
     3601 cgcgccggta gcatggcggc tcccgcagtg tcggggcgca tagctcagtt ggctagagca
     3661 tctggtttac atccagaggg tcgggggttc gagtccctct gcgcccaccg aaccggtgtg
     3721 ggc
//
LOCUS       DAOSOA010000327         3714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24868_length_3714_cov_4.409948, whole genome shotgun sequence.
ACCESSION   DAOSOA010000327 DAOSOA010000000
VERSION     DAOSOA010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24868_length_3714_cov_4.409948"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1776
                     /locus_tag="PKY13_15040"
     CDS             <1..>1776
                     /locus_tag="PKY13_15040"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOT81308.1"
                     /translation="ALVGTTLTGPAGSYTFTGLTPGGTYTVTETDPTGYGSSLHPGAT
                     AVTAPAADTGGPGPDFGDTLAELAGTVFVDVDRDGVRDAGEPGIVGATVTLDGTDATG
                     SAVTATTATATDGSYRFGDLLAGTYRITETQPWGHLDGAEVQGSIPSDTSVNDVHGAV
                     LVGAGVVGTGLDFGEHRAPSITTAISGRVFHDRGADGTTANGTYETGEPGLGGVTLIL
                     RDVNDVVVDSTVTASNGTYAFTGVPSGTYSVEKLQPAGYGISTPSLLGSLNVPIVGLD
                     DVDFGVTLGSLRGVVFSDVDDDGRLDSGESGIAGVALLVTGTDAFGNAVSEPATTDAN
                     GRFAVVDLLGSDPTGYTVTEAQPTGWVDGVDVSALTGVSGEDQFPGIVVAAGESLDVG
                     TFGEIAGVRLGDTVWNDADGDGVQDASEDGVGGMLVSLTGTTTRTGLPAVELSTTTAA
                     DGTYAFADLRPGTYRVAVTLGDRIVSYRGVGGDRTVDSDVDDDGRADAVTVVGNATVA
                     THESDRLDLDAGVSKGGSIRGRLFKDGNRSGTRNAGEAAGSCAEVRLTPAGPDRVFDS
                     ADDPKVVSTKPTNDGAYEFGPVLPG"
     assembly_gap    1777..1876
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1877..2164
                     /locus_tag="PKY13_15045"
     CDS             <1877..2164
                     /locus_tag="PKY13_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81309.1"
                     /translation="STKPTNDGAYEFGPVLPGRYRVGSDCTTTVEVEVLGEQIVNDVD
                     LPMEAAPSTLAFSGATVAWVLSFSVLLAGAGIVVLSVRPGIVRRRKQHPTT"
     gene            2311..3015
                     /locus_tag="PKY13_15050"
     CDS             2311..3015
                     /locus_tag="PKY13_15050"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT81310.1"
                     /translation="MTSPSVVIRRPVRHARPARRIGLAVVLALLAGLVLAMGAPSASA
                     QADPTTTTTADPNAPTTTAPPAAAPAPAPAPKLSDIFPGIDPSITVEQLEAFFRWIQG
                     PPVPANSGVGRRIVYSNSAQRVWLVEGDERVAKTYLVSGRRGLPAFGTYQVFSKSRTT
                     TSGSARMEFMVRFARGRNLAIGFHTIPLRGNGTPLQTESELGFPRSHGCVRQAPPDAM
                     YLYMWAPVGTTVVAIP"
     gene            3052..>3714
                     /locus_tag="PKY13_15055"
     CDS             3052..>3714
                     /locus_tag="PKY13_15055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT81311.1"
                     /translation="MDHETHCDLLEDELATTEAAFRAVDPAAPVPACPGWTVADLAKH
                     LGGVHRRATHLVATLATERDRAPQDPTRPGPDATADEWADWLADGGRRLVATLRAADG
                     AAPVWTWGDGTDVAWWTRRQLHETLVHRIDAQQAAGAEWSVDPEVAVDAIDEHLGNIA
                     ASAYFSSGVANLKGDGSLHLHATDTEGEWMIELRDDGFDISHAHGKGTVAARGAAGDL
                     LAA"
BASE COUNT          527 a         1368 c         1225 g          494 t
ORIGIN      
        1 acgccctggt cgggacgacc ctcaccggac ctgccggctc ctacaccttc acgggcctca
       61 cgcccggcgg gacctacacg gtcaccgaga ccgacccgac cggttacggc tcgtcgctcc
      121 acccgggcgc gaccgcggtg accgcaccgg cggccgacac cggcggacca ggccccgact
      181 tcggcgacac cctcgccgag ctcgccggaa ccgtgttcgt cgacgtcgac cgcgacgggg
      241 tccgcgacgc cggtgagccc ggcatcgtcg gagcgaccgt caccctcgac ggcaccgacg
      301 ccaccggctc ggccgtcacg gcgacgaccg caacggcgac cgacggctcg tatcgattcg
      361 gcgacctgct cgccggcacc taccggatca ccgagacgca gccgtggggc cacctcgacg
      421 gtgccgaggt gcagggctcg atcccatcgg acacctcggt gaacgacgtg cacggcgcag
      481 tcctcgtcgg cgccggtgtc gtcggcaccg gtctcgactt cggcgagcac cgtgcaccca
      541 gcatcacgac cgcgatctcc ggccgggtgt tccacgaccg cggcgccgac ggcacgacgg
      601 ccaacggcac gtacgagacg ggcgagcccg gtctcggcgg tgtcaccctg atcctgcgcg
      661 acgtcaacga cgtggtggtc gactcgaccg tgacggcatc gaacggcacc tacgcgttca
      721 ccggcgtccc ctccggcacc tacagcgtgg agaagctgca gcccgccggt tacggcatct
      781 ccaccccgtc actcctcggg tcgctcaacg ttccgatcgt cggtctcgac gacgtcgact
      841 tcggcgtcac cctcgggagc ctgcgcggtg tcgtgttcag cgacgtcgac gacgacgggc
      901 gcctcgactc cggcgagtcc gggatcgccg gtgtggcgct cctcgtcacc ggcaccgacg
      961 cgttcggcaa cgccgtcagc gaaccggcaa cgaccgatgc gaatggccgt ttcgcagtcg
     1021 tcgacctgct cggcagcgac cccaccggct acacggtcac cgaggcgcag cccaccggtt
     1081 gggtcgacgg cgtcgacgtc agcgccctca ccggcgtgtc gggcgaggac cagttcccgg
     1141 gcatcgtcgt ggcagcgggt gaatcgctcg acgtgggcac gttcggcgag atcgccgggg
     1201 tgcggctcgg cgacacggtc tggaacgacg ccgacgggga cggtgtgcag gatgcgtccg
     1261 aggacggcgt cggcgggatg ctcgtcagcc tgaccggcac cacgacccgg accggtcttc
     1321 ccgccgtcga actgtccacg acgaccgcgg cggacggcac gtacgccttc gccgatctgc
     1381 ggccgggcac ctaccgggtg gccgtcacgc tcggcgaccg catcgtcagc taccgcggag
     1441 tcggtggtga ccggacggtc gactccgacg tcgacgacga cgggcgcgcc gacgccgtga
     1501 ccgtggtcgg caatgcaacg gtggcgaccc acgagtcgga ccggctcgac ctcgacgccg
     1561 gcgtctccaa gggcggatcg atccggggcc gcctgttcaa ggacggcaac cggtccggca
     1621 cccgcaacgc tggcgaggcc gccggttcgt gcgccgaggt ccgcctcacg ccggccggcc
     1681 ccgaccgcgt gttcgactcg gcggacgatc cgaaggtcgt gtcgacgaag cccacgaacg
     1741 acggcgccta cgagttcggt ccggtcctgc cggggcnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnntcga cgaagcccac gaacgacggc gcctacgagt tcggtccggt
     1921 cctgccgggg cggtaccggg tcggctcgga ctgcacaacg accgtcgagg tcgaggtgct
     1981 cggcgagcag atcgtgaacg acgtcgacct gccgatggag gcagctccga gcacgctggc
     2041 cttctcgggt gccacggtgg cgtgggtgct gagcttctcg gtgctcctcg ccggtgccgg
     2101 catcgtggtg ctgagcgtgc gaccgggcat cgtccgccgc cgcaagcagc accccaccac
     2161 ctaacgaccc gcctcccggg gcttttgtga tgcgaggagc gacgctgacg tcgctcctcg
     2221 cactacaaaa ccccgagggt cgggcgaggg cgggcgttgg ccggttcggg gatcagccga
     2281 agtcgttcgg cgcgtcgata tcgtgaaccg atgacgtccc cctccgttgt catccgtcgc
     2341 cccgttcgcc acgctcgccc ggctcgtcgc atcgggctcg ccgtcgtcct tgcgctcctc
     2401 gccggtctcg tgctggccat gggtgcgccg tcggcgtcag cccaggccga cccgacgacg
     2461 accacgacgg ccgatccgaa cgcgccgacg accaccgcgc cgcccgctgc cgcgcctgct
     2521 cccgcgccgg ccccgaagct ctccgacatc ttccccggca tcgacccgtc catcacggtc
     2581 gagcagctcg aggcattctt ccgatggatc caggggccgc cggtcccggc caactcgggc
     2641 gtcggccgcc gcatcgtcta ctcgaacagc gcccagcggg tctggctcgt cgaaggcgac
     2701 gagcgcgtcg ccaagaccta cctcgtgtcg ggccgtcggg gcctcccggc gttcggcacg
     2761 taccaggtgt tcagcaagag ccgcaccacc acctccggct cggcgcgtat ggagttcatg
     2821 gttcgcttcg cccgcggtcg gaacctcgcc atcgggttcc acacgatccc cctccgcggc
     2881 aacggcaccc cgctgcagac cgaatcggaa ctcggatttc cacgctccca tggctgcgtc
     2941 cgccaggccc cgcccgacgc catgtacctg tacatgtggg cgccggtggg caccacggtc
     3001 gtcgcaattc cctgagcctt cgggcaacgc gggagtggca gaatcgccgg catggatcac
     3061 gagacgcact gcgacctcct ggaggacgag ctggcgacga ccgaggcggc gttccgcgcc
     3121 gtcgacccgg ccgcgcccgt cccggcgtgc ccgggctgga ccgtcgccga ccttgctaag
     3181 cacctcggcg gggtccaccg ccgggcgacc cacctcgtcg cgacgttggc caccgaacgc
     3241 gaccgggcgc cacaggaccc gacccggccc ggccccgatg ccacggccga cgagtgggcc
     3301 gactggctcg ccgacggcgg gcgcaggctc gtggccacgc tgcgagccgc tgatggtgcc
     3361 gccccggtgt ggacgtgggg tgacggcacc gacgtcgcgt ggtggacccg acgacagctg
     3421 cacgagaccc tcgtgcaccg catcgacgcc cagcaggcgg ccggggccga gtggagcgtc
     3481 gatcccgagg tcgccgtcga cgccatcgac gagcacctcg gcaacatcgc ggcgtcggcc
     3541 tacttcagca gcggcgtcgc caacctgaag ggcgacggat cgctgcacct gcacgcgacc
     3601 gacaccgagg gtgaatggat gatcgagctc cgcgacgacg ggttcgacat cagccacgcg
     3661 cacggcaagg gcaccgtcgc cgcccggggc gccgccgggg acctcctcgc cgcc
//
LOCUS       DAOSOA010000328         3712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_24881_length_3712_cov_6.327317, whole genome shotgun sequence.
ACCESSION   DAOSOA010000328 DAOSOA010000000
VERSION     DAOSOA010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24881_length_3712_cov_6.327317"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PKY13_15060"
     CDS             complement(<1..527)
                     /locus_tag="PKY13_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81312.1"
                     /translation="MRTAGGASRRAGIVIAVLVVSAVLSGCQSGKVGGRCKAGTVAQD
                     ATHVLVCQKGRWKRLISKADGLRKLAEIKAAEERAKLPPPTTAPVVDVPVVADPPTYQ
                     EPTDDPPAEAPSTNATVSTVLAAVADPPCSSCSLEFVVSASGRFVAFETNRAFDSGDT
                     DFAPDLYLLDTTTGSV"
     gene            complement(627..>2537)
                     /locus_tag="PKY13_15065"
     CDS             complement(627..>2537)
                     /locus_tag="PKY13_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT81313.1"
                     /translation="FVEVSLAYGRMLAEPDPQSAAEVYGLALDTLHDPPAEAEVDIAI
                     DLIRALRPVASRVGMSEAELDAIRRERRLLDAGLVHAEPGVREELCRQAAAVRFADGQ
                     VAEGFVELMDGLRSCGPDGLDDLATDLVLAVRRRHVPDELMADVENMWVELRRQRFEG
                     NWPLWCLTALGALRLDVGDVVTAQMYIRRAESILGHIERPVAGALVGGWVALAADDFG
                     RAHDRFTFATNAASPDDRMRLLAAAGLGEALICLGRPDDARRPLAEAISYDVGDPQSV
                     ARCHELLAEIASADGRYADAYQHLQVTRRLEQVARGGSAPVERMRTASPAVEPPIAPA
                     PTSLPESEVVDLRDDSADSVTTVDLTPTVDLTPSAPAPSAPPTDHVMELSEDPARRMV
                     LEALRNNWFELYFQSIDDNTRHRPGAAEALLRMNHPDHGVLRPEHFLPGLTDRQIAEE
                     LCLWTVTRACEALARWNAGGAVFAVVVNVSEAQLTPALPVIVRTALARSGARADLLTL
                     EVDGSSAGRIDADQIGVLNEVRATGARIGIDRFGSEFRSIDTLLKLPVDVVKFDRSVF
                     GGGALQPLERRMVDTVIALARAFGFEVIATGIETPAQLDLQEQFGCHGAQGYLLGIPV
                     PEPEFRQLLNVA"
     assembly_gap    2538..2637
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2638..>3712)
                     /locus_tag="PKY13_15070"
     CDS             complement(<2638..>3712)
                     /locus_tag="PKY13_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81314.1"
                     /translation="RVATKQEVAERLLAYLHATPTVIAASDGHACASMLDRTVRCWGR
                     NTSGQLGNATTTSSSTPVDVVGLRIESSWSTGPGRSGVTGPGALALGDAYSCGISPGR
                     AGTTSTTTYWCWGAVRDPTDIVTKPELIGGGGTPGIASLVFGPTAHCESLELNYGYRC
                     SIYQLILPSGTKLATAGRAHECLVASNDMLYCVGANDRGQLGNGSTTDIPFVPYPSTL
                     AATGLTDARIVDAGGDSTCAVVGASDVWCWGGNGRGQLGDGTTTDRTLPTRVAGLSDV
                     VSLSVGTDHACAAGGSGVLRCWGANTSGQLGDSTTIDHPLPTVVSGLTNVTSVTAGAG
                     STCAIGDRGTAKQQVFCWGRNDWG"
BASE COUNT          545 a         1260 c         1310 g          497 t
ORIGIN      
        1 accgaccccg tggtcgtgtc gagcaggtag aggtccggtg cgaaatcggt gtcaccggag
       61 tcgaaggccc ggttcgtctc gaaggcgacg aagcggcccg acgccgagac cacgaactcg
      121 agcgaacagc tcgagcacgg cggatcggca accgcggcga gcaccgtgga cacggtggcg
      181 ttggtcgacg gcgcttcggc cggcgggtcg tcggtcggct cctggtacgt cggcggatcg
      241 gcaacgaccg gcacgtcgac gaccggggcc gtcgtcggag gtgggagctt ggcccgctcc
      301 tcggccgcct tgatctcagc cagcttgcgg aggccgtcgg ccttggagat gaggcgcttc
      361 cagcggccct tctggcagac cagcacgtgg gtggcgtcct gggcgaccgt gcccgccttg
      421 caccggcctc cgaccttgcc tgattggcat ccgctcagca cggccgacac cacgagcacc
      481 gcgatcacga tcccggcgcg tcgcgacgct cctcctgctg tcctcatcgc catctcccaa
      541 cggggcgccc aacgcctcgt gggaccgtac cgacggccca ccccgatgac aatcccctac
      601 cggcgatgac cgaaaccggg agaccgctac gcgacgttga gcagctgccg gaactccggc
      661 tccggcaccg ggatgccgag gaggtagccc tgggcgccgt ggcagccgaa ctgctcctgc
      721 aggtcgagtt gcgccggcgt ctcgatgccc gtggcgatca cctcgaagcc gaaggcccgg
      781 gcgagggcga tcacggtgtc gaccatgcgc cgctcgagcg gttggagtgc accaccgccg
      841 aacaccgacc ggtcgaactt cacgacgtcg acggggagct tgagcagggt gtcgatcgag
      901 cggaactcac tcccgaaccg gtcgatgccg atgcgggcac cggtggcccg cacctcgttg
      961 agcacgccga tctgatcggc gtcgatccga cccgcggacg acccgtcgac ctcgagcgtc
     1021 aggaggtcgg cccgggcgcc ggaccgtgcg agtgctgtgc ggacgatcac ggggagggcc
     1081 ggggtgagct gtgcctcgga cacgttcacc accaccgcga agacggcacc gccggcgttc
     1141 caacgggcca gggcctcaca cgcccgcgtc accgtccaca agcagagctc ctcggcgatc
     1201 tgacggtcgg tgaggccggg caggaagtgc tcgggccgga gcacaccgtg gtcggggtgg
     1261 ttcatccgga ggagcgcctc ggcagcgccg gggcggtggc gcgtgttgtc gtcgatcgac
     1321 tggaagtaca gctcgaacca gttgttgcgc agtgcctcga gcaccatccg acgagccgga
     1381 tcctcggaca actccatcac gtggtcggtc ggcggcgccg acggtgcggg ggcggacgga
     1441 gtgagatcga ccgtgggggt caggtcgacg gtcgttacgg aatcggccga gtcatcgcgc
     1501 aggtcgacga cctcggactc gggcagcgac gtcggggccg gggcgatcgg cggctccacc
     1561 gccggactcg cggtgcgcat ccgctccacg ggcgcagatc cgccacgtgc cacctgttcg
     1621 agacggcggg tcacctgcag gtgctgatag gcgtcggcat accgaccgtc ggcggaggcg
     1681 atctcggcga gcagctcgtg gcagcgggcc accgactgcg ggtcaccgac gtcgtaggag
     1741 atcgcctcgg cgagcgggcg acgcgcatcg tcgggccggc cgaggcagat cagcgcctcg
     1801 ccgaggccgg cggccgcgag caggcgcatg cgatcgtcgg ggctcgcagc gttcgtggcg
     1861 aacgtgaagc ggtcgtgggc tcgaccgaag tcgtcggcgg cgagcgccac ccatccgccg
     1921 acgagcgcgc ccgccaccgg tcgctcgatg tggccgagga tgctctcggc gcgacggatg
     1981 tacatctgcg cggtgacgac gtcgccgacg tcgaggcgaa gggccccgag cgcggtcagg
     2041 caccacagcg gccagttgcc ctcgaagcgc tgacgccgga gctcgaccca catgttctcg
     2101 acgtcggcca tgagctcgtc gggcacgtgc cgacgccgga cggccagcac gagatcggtg
     2161 gcgaggtcgt cgaggccgtc ggggccacac gaccgcagac catccatcag ctcgacgaac
     2221 ccctcggcga cctggccgtc ggcgaaccgc acggccgccg cctgccggca cagttcctcg
     2281 cggacacccg gctcggcgtg caccaggccg gcgtcgagca accgacgctc gcgacggatc
     2341 gcgtcgagct ccgcctcgga catgccgacg cggctcgcga cgggacggag cgcacgaatg
     2401 aggtcgatcg cgatgtcgac ctcggcctcg gccggcgggt cgtgcaacgt gtcgagcgcc
     2461 aggccgtaca cctcggcggc cgactgcggg tcgggttcgg cgagcatccg gccgtacgcg
     2521 aggctcacct cgacgaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     2641 cagtcgttgc ggccccagca gaacacctgc tgcttcgccg tccctcgatc gccgatcgcg
     2701 cacgtcgagc cagcgcccgc ggtgacgctg gtgacgttgg tgaggcccga tacgacggtg
     2761 ggcaacggat ggtcgatcgt cgtcgagtcg cccagctggc cgctcgtgtt ggcgccccag
     2821 cagcggagga cgccgctgcc gccggcggca cacgcgtggt cggtgccgac ggagagcgag
     2881 acgacgtcag agagtccggc gacccgcgtc gggagggtgc ggtcggtcgt cgtgccgtcg
     2941 ccgagctggc cgcgtccgtt gccgccccag caccacacgt cgctcgcgcc cacgacggcg
     3001 caggtcgagt cgcctccggc atcgacgatc cgcgcgtcgg tgagacccgt tgcagccaat
     3061 gttgacgggt acggcacgaa cgggatgtcg gtcgtcgacc cgttgccgag ctgaccgcgg
     3121 tcgttggcgc cgacgcagta cagcatgtcg ttcgacgcca cgaggcactc gtgagcccgg
     3181 ccggccgtgg ccagcttggt gcccgaaggc aggatgagct gatagatcga gcaccggtac
     3241 ccgtagttga gctcgagcga ctcgcagtgc gcggtcggac cgaacaccaa cgacgcgatg
     3301 ccgggcgtgc cgccaccgcc gatgagctcg ggcttggtca cgatgtcggt cggatcgcgc
     3361 accgcgcccc agcaccagta ggtcgtcgtc gacgtcgtgc cggcgcgacc agggctgatg
     3421 ccgcagctgt acgcgtcgcc gagcgccagc gcgcccggcc cggtgacacc cgaacggcct
     3481 gggccggtcg accagctgct ctcgatccgc aggcccacga cgtcgaccgg tgtcgagctg
     3541 ctggtcgtgg tcgcgttgcc caactggccg gaggtgttgc gcccccagca gcgcactgtc
     3601 cggtcgagca tcgacgcgca ggcgtggccg tcgctggccg cgatcacggt cggcgtcgcg
     3661 tggaggtacg cgagcagccg ctcggcgacc tcctgcttgg tcgccacgcg gg
//
LOCUS       DAOSOA010000329         3646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_25475_length_3646_cov_7.050404, whole genome shotgun sequence.
ACCESSION   DAOSOA010000329 DAOSOA010000000
VERSION     DAOSOA010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25475_length_3646_cov_7.050404"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKY13_15075"
     CDS             <1..690
                     /locus_tag="PKY13_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81315.1"
                     /translation="EGLVAVTSVRLDWPGFEAAPPTVRPVQVAPGQRVDLPTPLGAVA
                     CTTADTPPFSLGAVEVTVDPGDGTARTVRAPLLADAQTALDNVWRTACVRQRLLDRVG
                     LEYSAWVRSDPDGVPTAAGSLVVTRRDTSDAISVTGVDGSVLLFVNWTAPPPTLPTDG
                     RQLEVPIVVTGAGRCDGHALSDSKKTYVFQVQVQVGDEEPVGVDLPVPDADRPLLNEI
                     IVDTCGSDHRR"
     gene            815..1999
                     /locus_tag="PKY13_15080"
     CDS             815..1999
                     /locus_tag="PKY13_15080"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOT81316.1"
                     /translation="MAGSVILSGARTPIGKLAGSLAGFSGTDLGGLAIKAALERAGLS
                     GDQVDYVFMGQVLLAGAGQMTARQAAVKGGIPMSVPSTTINKVCLSGINSIMMADLLI
                     QAGQADVVVAGGMESMTNAPYLLPDARSGMRMGDKKVIDSMMFDGLFCAIDQVPMGGG
                     TEKYAGAAGLTRDEQDQVAYLSNERAARAQKDGLFDNEIVNVEIPQRKGDPVVFSSDE
                     GVRGDTTLDSLGKLRPAFDKEGNITAGNASQISDGGAAVVVTSKAFAEKAGITPLGEI
                     LGNGQVAGPDASLLTQPSRAIFQALERAGKTVSDVDLFELNEAFAAVGVASMRDLGIT
                     DEIVNVNGGAIALGHPIGMSGTRIVLTVLHELARRGGGIGAAALCGGGGQGDALLVKT
                     IS"
     gene            2088..2954
                     /locus_tag="PKY13_15085"
     CDS             2088..2954
                     /locus_tag="PKY13_15085"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOT81317.1"
                     /translation="MSEPVVEQIWRYPAKSMLGERLREVELTTAGLLGDRGWAVRDEV
                     RGGIRGAKKIAELMLLGARYLAPPSDRNATPHIEITLPDGARVASSDPDVDERVSAAL
                     DHQVTLWPLQPSTDLDHYRRGSADSDDFLQEMRDIFGRLEDEPLPDLSVFPPEILEYE
                     SPPGTYLDAFPLLVLTTGSMQSLQDRLPESAIDVRRFRPNLLVRADADGDQAWPELDW
                     VGRQATLGGATLEFVTPCPRCVMITRPVAELPSDRSIMRTVVRDADQNLGVYATVVTP
                     GEVHEGDELIFV"
     gene            3086..>3646
                     /locus_tag="PKY13_15090"
     CDS             3086..>3646
                     /locus_tag="PKY13_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81318.1"
                     /translation="MGKKLAIIGLSAGLLGGGAAGLAFTGTTGIAGAQDTTSTTVAAD
                     TPADASTGTADADRPDPSTKLAEALAPLVTDGTLTQAQADKVVETLKAAGPVGGGGHG
                     PGRDGHGGRGGGPGSEAAATALGITADELRTELQGGATIAELATEKGVELSTVTDAML
                     AAEKEHLAEEVTAGKITQAQADERLSG"
BASE COUNT          572 a         1332 c         1211 g          531 t
ORIGIN      
        1 gaggggctcg tcgcagtgac gtcggtccgg ctcgactggc ccggcttcga agcggcgcca
       61 cccacggtcc gccccgtgca ggtcgccccg ggccagcggg tcgaccttcc gacgccgctc
      121 ggtgccgtgg cgtgcacgac cgccgacacc ccgccgttct cgctcggcgc cgtggaggtg
      181 accgtcgacc ccggcgacgg aaccgcccga acggtcaggg caccgttgct cgccgacgcg
      241 cagacggccc tcgacaacgt gtggcgcacg gcgtgcgtcc gacagcgcct cctcgatcgt
      301 gtcggtctcg agtactcggc ttgggtacgg tcggatcccg acggagttcc cacggcggcg
      361 ggctcgttgg tcgtgacccg ccgtgacacg tccgacgcga tcagcgtcac cggagtcgat
      421 ggcagcgtcc tgctgttcgt gaactggacg gcgccgccgc ccacactgcc gacggacggc
      481 cgtcagctcg aggtgccgat cgtggtcacg ggggccggac gctgcgacgg ccacgcgttg
      541 agcgactcga agaagaccta cgtgttccag gtgcaggtcc aagttggtga cgaggaaccc
      601 gtcggtgtcg acctgcccgt ccccgatgcc gaccgtccac tgctcaacga gatcatcgtc
      661 gacacctgcg gcagtgatca ccggcgttga tggtggccat ctcccgatgt gatcggtgtc
      721 acccccgtcg ccgttggccg acggcggtta ccggccggta acgtcgtctg attccgggcc
      781 accgggccca accatccaaa caaggggaag tgcaatggcc ggatcggtga ttctcagcgg
      841 ggcgcgtacc ccgatcggca agttggcggg ctcgctcgcc ggattcagcg gtaccgatct
      901 cggcggtctc gccatcaagg ccgccctcga gcgcgccggc ctctccggcg accaggtcga
      961 ctacgtgttc atgggccagg tgctcctcgc cggcgccggc cagatgacgg cccgccaggc
     1021 ggccgtcaag ggcggcatcc ccatgtccgt cccgtcgacg accatcaaca aggtctgcct
     1081 gtcgggcatc aactcgatca tgatggccga cctcctcatc caggccggcc aggccgacgt
     1141 cgtcgtcgcc ggtggtatgg agtcgatgac caacgccccg tacctcctcc ccgacgcccg
     1201 cagcggcatg cgcatgggcg acaagaaggt gatcgactcg atgatgttcg acggcctctt
     1261 ctgcgccatc gaccaggtcc ccatgggtgg cgggaccgag aagtacgccg gagccgccgg
     1321 gctcacccgc gacgagcagg accaggtcgc ctacctctcc aacgagcgtg cggcccgtgc
     1381 ccagaaggac ggtctgttcg acaacgagat cgtcaacgtc gagatccccc agcgcaaggg
     1441 cgacccggtc gtgttctcgt ccgacgaggg cgtccgcggc gacaccacgc tcgactcgct
     1501 cggcaagctc cgccccgcct tcgacaagga aggcaacatc accgccggca acgccagcca
     1561 gatctccgac ggcggcgctg ccgtggtcgt gacctcgaag gcgttcgccg agaaggccgg
     1621 catcaccccg ctgggcgaga tcctgggcaa cggccaggtg gccggtcccg acgcctcgct
     1681 cctcacccag ccgagccgtg cgatcttcca ggcgctcgag cgcgccggca agaccgtgtc
     1741 cgacgtcgac ctgttcgagc tcaacgaagc cttcgccgcc gtcggtgtcg cctcgatgcg
     1801 cgacctcggc atcaccgacg agatcgtcaa cgtcaacggt ggcgccatcg ccctcggcca
     1861 cccgatcggc atgtccggca cccgcatcgt gctcacggtc ctccacgagc tcgcccgtcg
     1921 cggcggcggc atcggcgccg ccgcactctg cggtggcggc ggtcagggcg acgccctcct
     1981 ggtcaagacc atctcctgac ccgagctctg cacgttctcc cgacgtggaa cccgctgggc
     2041 gcgggtttcc cgtcgggagt tcgtcgtttt gggcgtaggt tgcgtccatg agcgaacccg
     2101 tcgtcgagca gatctggcgc taccccgcga agtcgatgct gggcgagcga ttgcgtgagg
     2161 tcgagctgac gaccgcagga ctgctgggcg accggggatg ggccgtgcgc gacgaagtcc
     2221 gaggtggcat ccggggcgcg aagaagatcg ccgagctgat gctgctggga gcccggtacc
     2281 tggcgcctcc gtccgaccgc aacgcgaccc cgcacatcga gatcaccctg cccgacggcg
     2341 cccgcgtcgc ctcatcggac cccgatgtcg acgagcgtgt ctcagcggcc ctcgatcacc
     2401 aggtcaccct gtggccgctg caaccgtcga ccgatctcga ccactatcgg cgggggtcgg
     2461 ccgactccga cgatttcttg caggagatgc gagacatctt cggccggctg gaggacgagc
     2521 cactccccga tctcagcgtc ttcccgcccg aaatcctcga gtacgagtcg ccacccggca
     2581 cctacctcga cgcgttcccg ttgctcgtac tcaccaccgg gtcgatgcag tccctgcagg
     2641 atcgactgcc cgagtcggcc atcgatgtgc gacggttccg gccgaacctg ctggtccggg
     2701 cggacgccga cggcgaccag gcgtggccgg agctcgactg ggtcggccgg caggcgaccc
     2761 tcggcggcgc gaccctcgag ttcgtcactc cgtgccctcg ctgcgtgatg atcacgcgcc
     2821 cggtcgccga gttgccgagc gaccgttcga tcatgcggac cgtggtacgt gacgccgacc
     2881 agaacctcgg cgtgtacgcc accgtggtga cgcccggcga ggtgcacgag ggtgacgagt
     2941 tgatcttcgt gtgacccgca acggtggcgt cggagagtct tacgcacttc tgacgtagat
     3001 ctggcggtcg gctcacgttg gggcccgaag gtggagacag ttccggatcg accgggaccg
     3061 aaccccaccc aaggagaccc accacatggg caagaaactc gccatcatcg gactcagcgc
     3121 cggcctgctc ggcggcggcg ccgccggcct cgccttcacc ggaaccaccg ggatcgccgg
     3181 agcccaggac accacgtcga ccacggtcgc cgccgacacc cccgccgacg catccaccgg
     3241 gacggccgac gccgaccgtc ccgacccgtc gaccaagctc gccgaagcgc tcgccccgct
     3301 cgtcaccgac ggcaccctca ctcaggcgca ggccgacaag gtcgtcgaga cgctgaaggc
     3361 ggccggaccg gtgggtggcg gcgggcacgg cccgggccgc gacggtcacg gcggtcgcgg
     3421 tggcggcccc ggttccgagg cggcggcgac ggcgctcggc atcacggccg acgagctccg
     3481 caccgagctc cagggcggag cgaccatcgc cgagctggcg accgagaagg gcgtcgagct
     3541 ctccacggtc accgacgcga tgctcgccgc cgagaaggag cacctcgccg aagaggtcac
     3601 cgccggcaag atcacccagg cacaggccga cgagcgcctc agcggc
//
LOCUS       DAOSOA010000330         3615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_25797_length_3615_cov_5.495506, whole genome shotgun sequence.
ACCESSION   DAOSOA010000330 DAOSOA010000000
VERSION     DAOSOA010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25797_length_3615_cov_5.495506"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..2462
                     /gene="pheT"
                     /locus_tag="PKY13_15095"
     CDS             57..2462
                     /gene="pheT"
                     /locus_tag="PKY13_15095"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT81319.1"
                     /translation="MKVLHNWLAEYVPGLLDHPVGGDPDALGDVMSSLGLCCEEVVRI
                     GGGLDGIVVAEVLGLSPHPNADKIQLVQVRAEPGAEPLQVCCGAFNMAVGDLVPLATI
                     GTTMPGGMEIARRKLRGEYSNGMLCAEDELGLGADHAGILILAPALTVGTPITEALGI
                     VPDAMFDLDLTPNRPDALSVIGVARDVAAKLGLEFVLPAIDVPTSGTDAASLASVRID
                     DADLCGRFTVRVLSGVSIGESPSWMQQRLTHCGMRPINSVVDVSNYVMLEYGQPNHTY
                     DLATVPDGALGVRRARAGERLVTLDSQTRSLDERDGVIVNRDDEPIGIAGVMGGESTE
                     IGDSTESVLLELAWWDPASISFTSKRLGLRSEASSRYEKGVDHEIANRAAWRFAQLLV
                     EQGATLHPGEIVAEGNAPEPARIEVRTARVNALLDTEFTSDEIVAYLAPIGFAAEPTA
                     DPDVLAVTIPSWRPDSTGEIDVVEEVARLHGYEHLGKTVPKSSRAGGLTERQADIRRL
                     RSYLASTGASEATPMSFLAPGDLEASGVRADGVTVANPLVAEESVLRTSLLPGLVKTL
                     GYNAAHRNVGVSLFEIGHCFARSADGGLPTEWEEVAVVLGGQDARVAVELALRLATLI
                     GVEDVRLRNEPVGGLHPGRSAVIVAGEAAIGVVGEIDPEVCARRGVAERVGYVSFVLG
                     GDEYWHGDVGLLSAPRTDRVFRSISRMPSSDVDLAFVVDDSVGADELADTLRSSGGDL
                     VTSVDLFDVYRGTSVGDGRRSLAYRLRIQAPDRTLTDVELADVRNALVTAASTAHGAE
                     LRA"
     gene            2494..3525
                     /gene="argC"
                     /locus_tag="PKY13_15100"
     CDS             2494..3525
                     /gene="argC"
                     /locus_tag="PKY13_15100"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOT81320.1"
                     /translation="MQDGSVKIGIIGASGFAGSEVLRLTASHPSLELAWATGDSQAGT
                     AIADLYPSLAAAYGGQRFVPYDTALLADVDAVFLALPHGASQALVPELVGRVRWVIDL
                     AADFRLQEASLYDQWYGISHTAPELLPSFAYGLPELYRDHVAEAQCVAAPGCYPTAAI
                     LALEPLVRTGIIEPTGVIVDAASGVSGAGRPPKPNTTFCTVDEDFTAYGLLDHRHTPE
                     MEIHSGASVLFTPHLAPMSRGILATCYGRPARQGLSTVSVMDALHDAYAGEPFVVVSD
                     RVPSTKATQGSNCAHLSARYDDRTGTVIVLSAIDNLVKGTAGQAIQCLNLMAGLGETT
                     GLPVVGLTP"
BASE COUNT          517 a         1253 c         1239 g          606 t
ORIGIN      
        1 gttctagcga ctccggttcc cgacggacgc agtcccacga aagggctcac gctgcaatga
       61 aggttctcca caactggctc gcggagtacg tccccggtct gctcgaccat cccgtcggcg
      121 gcgaccccga cgcgctcggt gacgtcatga gctcgctcgg gctgtgctgt gaggaagtcg
      181 tccgcatcgg cggtggtctg gacggcatcg tggtggccga ggtgctcggg ctctcgccac
      241 accccaatgc cgacaagatc cagctcgtcc aggttcgggc cgagcccggc gccgaaccgc
      301 tgcaggtctg ctgtggtgcg ttcaacatgg cggtcggtga cctcgtcccg ttggcgacca
      361 tcggcacgac gatgccgggc ggcatggaga tcgcccgccg caaactccgc ggcgaatact
      421 cgaacggcat gctctgcgcc gaggacgagc tgggcctcgg cgccgatcac gccggcatcc
      481 tgatcctcgc tccggcgctc acggtgggca ccccgatcac cgaggccctg ggcatcgttc
      541 ccgacgcgat gttcgacctc gatctcaccc ccaaccgtcc cgatgccctg tcggtcatcg
      601 gggtcgcccg cgacgtggcg gcgaagctcg gcctcgagtt cgtgctgccc gccatcgacg
      661 tcccgacctc gggcaccgac gccgcctcac tggcgtcggt gcgcatcgac gacgccgacc
      721 tctgcggccg cttcaccgtc cgggtgctgt cgggtgtgtc gatcggcgag tccccgtcgt
      781 ggatgcaaca gcggctgacc cattgcggaa tgcgaccgat caactcggtc gtcgacgtgt
      841 ccaactacgt gatgctcgaa tacgggcagc ccaaccacac ctacgacctc gcgaccgtcc
      901 ccgacggtgc gctgggggtt cgtcgggcgc gggcggggga gcgactcgtc acactcgaca
      961 gccagacgcg ctcgctcgac gagcgtgacg gtgtgatcgt gaaccgagac gacgagccca
     1021 tcggcatcgc cggcgtcatg ggtggcgagt cgaccgagat cggcgattcc accgagtcgg
     1081 tgctgctcga gctggcgtgg tgggatcctg cgtcgatctc gttcacgtcc aagcggctgg
     1141 gtctccgttc ggaggcgtcg tcgcgatacg agaagggcgt cgaccacgag atcgccaacc
     1201 gggcggcgtg gcggttcgcc caactcctcg tcgagcaggg cgcaacgttg caccccggcg
     1261 agatcgtggc ggagggcaac gcccccgaac cggcccgcat cgaggtgcgc acggcgcgtg
     1321 tcaatgcgct gctcgacacg gagttcacgt ccgacgagat cgtggcgtac ctcgctccga
     1381 tcggcttcgc tgctgagccg acggccgatc ccgacgtcct cgcggtcacg atcccgtcgt
     1441 ggcggcccga ctcgaccggc gagatcgacg tcgtcgagga ggtggcccgt ctccacgggt
     1501 acgagcacct cggcaagacc gtgccgaagt cgtcgcgggc cggcgggctg accgaacgcc
     1561 aggccgatat ccgccgactc cggtcctacc tggcgtcgac cggtgcctcg gaggcgacgc
     1621 ccatgtcgtt cctcgctccg ggcgacttgg aggcgagtgg tgtgcgtgcc gacggtgtga
     1681 ccgtcgcgaa cccgctcgtc gccgaggaat cggtgctgcg cacgtcgctc ctcccggggc
     1741 tcgtcaagac gctcggctac aacgcggcgc accgcaacgt cggggtgtcg ttgttcgaga
     1801 tcggacactg cttcgcccgc tccgccgacg gtggactgcc caccgagtgg gaggaggtcg
     1861 cagtggtgct cggcggtcag gatgcccgcg tcgcggtcga actggcgctg cggttggcga
     1921 cgttgatcgg cgtcgaggac gttcggctgc gcaacgaacc ggtcggcggg ctgcatccgg
     1981 gccgatcggc ggtcatcgtc gcaggcgagg ccgcgatcgg cgtcgtcggc gagatcgatc
     2041 cggaggtgtg cgcccgtcgt ggggtcgccg agcgggtggg gtacgtctcc ttcgtcctcg
     2101 gtggcgatga gtactggcac ggtgacgtcg gtctgttgtc ggcgccgcga accgaccgcg
     2161 tgttccggtc gatcagccgg atgcccagct ccgatgtcga cctggcgttc gtggtggacg
     2221 actccgtcgg agccgacgaa ctcgcggaca cgctgcggtc ctccgggggc gatctcgtga
     2281 cctccgttga cctgttcgac gtgtaccggg gcacctcggt cggcgacggc cggcgcagtc
     2341 ttgcctatcg tttgcggatc caggcgcccg accgcaccct gaccgacgtc gagctggcag
     2401 acgtccgcaa cgcgttggtc acggcggcct cgacggcgca cggagccgaa ctccgcgcct
     2461 gaacgtcata gtcatgcgct aaggtgcatg actatgcagg acggatcagt caagatcggc
     2521 atcatcggcg cgtcggggtt cgcggggtcc gaggtgctgc gcctcaccgc gtcgcacccg
     2581 tcgctggagc tggcgtgggc gaccggagat tcccaggcgg ggaccgcgat tgccgatctc
     2641 tacccgtccc tcgccgccgc ctacggcggg caacggttcg tcccgtacga cacggcgctg
     2701 ctcgccgatg tcgacgccgt cttccttgcc ctgccccacg gggcatcaca ggcgctggtc
     2761 cccgagctgg tcggtcgtgt gcggtgggtg atcgatctgg ccgccgactt ccggctgcag
     2821 gaagcgtcgc tctacgacca gtggtacggc atcagccaca cggcccccga gctgctcccg
     2881 tcgttcgcgt acggcctgcc cgagctgtac cgcgaccatg tcgccgaggc gcagtgcgtc
     2941 gctgctccgg ggtgctatcc gacggctgcg atcctggcgc tcgaaccact ggtccgcacc
     3001 ggcatcatcg agccgaccgg tgtgatcgtc gacgccgcgt cgggcgtgtc gggtgccggc
     3061 cgcccgccca agccgaacac cacgttctgc accgtcgacg aggacttcac ggcctacggg
     3121 ctgctcgacc accgacatac gccggagatg gagatccact cgggcgcgtc ggtcctgttc
     3181 acaccacacc tggccccgat gagtcgcggc atcctggcga cgtgttacgg ccgaccggca
     3241 cgccagggcc tgtcgacggt gtcggtcatg gacgcactgc acgacgcgta tgccggcgag
     3301 ccctttgtgg tcgtgtcgga ccgggtgccg tcgaccaagg cgacccaggg ttccaactgc
     3361 gcgcacctat cggcgcgcta cgacgatcgg accggcaccg tcatcgtgct cagcgccatc
     3421 gacaatctcg tgaagggcac cgccggccag gccattcagt gcctcaacct gatggcgggc
     3481 ctcggcgaga ccaccggtct ccccgtggtc ggactcaccc cctgacgcgt tctgtcccgc
     3541 gagggcgtgc aggctgcacg tgtcctgtcg cgggacatgg gtgacacata tgtcgcggga
     3601 catgggtgac acgtc
//
LOCUS       DAOSOA010000331         3597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_25957_length_3597_cov_5.560700, whole genome shotgun sequence.
ACCESSION   DAOSOA010000331 DAOSOA010000000
VERSION     DAOSOA010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25957_length_3597_cov_5.560700"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKY13_15105"
     CDS             complement(<1..592)
                     /locus_tag="PKY13_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOT81321.1"
                     /translation="MAATFIDDPADPRVADFRRLNDGAFRRTVEAAGPFHKGMFVVEG
                     WLGLERLVPSRYPIRAVLVDAAKVARAEEVLGRSPAPLLVADRALLDEIVGFPLHRGV
                     VAVADRGLPVLSSTVVGRGRRLLVTEGVNDAENLGSIIRNAVALGGDGLLLDPTSCDP
                     LTRRTVRVSVGHALALPFARLAWPDGLATLRESGLTT"
     gene            complement(637..1398)
                     /locus_tag="PKY13_15110"
     CDS             complement(637..1398)
                     /locus_tag="PKY13_15110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT81322.1"
                     /translation="MTIRQQPAAGVPIWIDLMTSDRDASVAFYTELFGWTSDEPNPDF
                     GGYSNFRLHGEEVAGLMTAEPGAPSDMWSVYLATPDAPATLEAARNAGASVMVDAMAV
                     GDLGTMAVVVDPGGAVIGMWQPGEHRGGVVATTNAPCHFELHTTSYDAVLPFYRDVFG
                     WTISTAADEPGFRYSTYDVADGENAGIMDASVFGEGASNCWSVYFAADDVAATLARAV
                     ELGGAVLHGPETTPYGVLATATDATGALFKLRGDA"
     gene            1536..2411
                     /gene="hisG"
                     /locus_tag="PKY13_15115"
     CDS             1536..2411
                     /gene="hisG"
                     /locus_tag="PKY13_15115"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOT81323.1"
                     /translation="MLNLVLPKGSLEKATFQLFSDADLGIHRNSAVTYKATIADPRIA
                     SVRILRPQEIPVYVADGLFDLGVCGRDWVEETSSNVVSLGEMGYSKATTNPIKMVVAV
                     AGDAEWEKVADLPQGVKVSTEYPEITRRFFEQNGIEADVRLSYGATEAKVPDIVDCVV
                     EITETGNALRAAGLKVIDTILHSYTEVVANPATYEVPEKRHAMEQIMTLLQGVLEARS
                     KVLVKLNVSTDDLDAVIAKLPAMKTPTVNELYRRNGYAVETVVPKNEINILIPALKDA
                     GATDILELPLAKIVH"
     gene            2411..2617
                     /locus_tag="PKY13_15120"
     CDS             2411..2617
                     /locus_tag="PKY13_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOT81324.1"
                     /translation="MTVRTGTVTEFDDPRGIGVVTGADGTEYPFHCTAILDGTRQIDV
                     GASVRFVDRPGRQGRWEATEIAPV"
     gene            complement(2636..>3597)
                     /locus_tag="PKY13_15125"
     CDS             complement(2636..>3597)
                     /locus_tag="PKY13_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81325.1"
                     /translation="ILALTNLTGASFDFGTVFGFGAGDPAPPACGAYCPGYNEATVDT
                     GREICDEGYGARFGIPDFYLTRSSSTLATEGRRSVVTDAATDFRGATFGGPGNLLSSL
                     ALRGLSLAEADFSDATFDGQVLGCRDVSGGRFERAHLTGVQLIDVDATGADFGAALFD
                     GGSKLCALDLTNADLDGARFWGGTGISCTDLGDDLRSALPALVVFDSASMRGVQFGEP
                     DGNGQRVTWRNLSSAVPTPGVSADLAALSGSAANADFSGAVFGSTTIGPFAVLGSGQW
                     DDIDFRTSDWTGAEFHGTQSFVGSTCPDGSTGDATTPCSPLLP"
BASE COUNT          612 a         1227 c         1236 g          522 t
ORIGIN      
        1 cggtcgtcag tccggattcc cggagcgttg cgagcccgtc gggccacgcc aggcgggcga
       61 acggcagcgc cagggcgtgg cccactgaca cccgcaccgt tcgtcgggtc agcgggtcac
      121 atgacgtcgg gtcgagcaac aggccgtcgc ccccgagcgc cacggcgttg cggatgatcg
      181 aaccgagatt ctcggcgtcg ttgacaccct cggtgacgag gagtcggcga ccgcgaccga
      241 cgacggtcga tgacagaacc ggcaatcccc ggtcggccac tgcgacgaca ccccggtgga
      301 gtgggaaccc gacgatctcg tcgagcaggg cccgatcggc gacgagcagg ggagcggggc
      361 tccgcccgag cacctcctcg gcccgagcga ccttcgcggc atcgaccaac acggccctga
      421 tcgggtagcg cgacggcacc agccgttcga gtcccaacca cccctcgacg acgaacatgc
      481 ccttgtggaa cgggccggcc gcctcgacgg tacgccggaa cgcaccgtcg ttgaggcgcc
      541 ggaagtcagc gaccctcggg tcggcggggt cgtcgatgaa cgttgcggcc acgggaccca
      601 gggtaggtgg tggcgccccg ggtcccgtgg tgcggatcag gcgtcgccgc gcagcttgaa
      661 gagcgccccc gtggcgtcgg tcgcggtcgc caacaccccg tacggagtgg tctcgggccc
      721 atggagcacc gcaccgccga gttcgacggc ccgggcgagg gtcgccgcca cgtcgtcggc
      781 cgcgaagtac accgaccagc agttgctggc tccttctccg aacaccgacg catccatgat
      841 cccggcgttc tcgccgtcgg ccacgtcgta ggtcgagtag cggaagcccg gttcgtcggc
      901 cgccgtgctg atcgtccacc cgaacacgtc gcggtagaag gggagcactg cgtcgtagga
      961 ggtggtgtgc agttcgaaat ggcacggagc gttcgtggtc gccacgacgc cgccccggtg
     1021 ttcacccggt tgccacatgc cgatgaccgc accgccggga tcgacgacga ccgccatggt
     1081 gccgaggtcg ccgacggcca tggcgtccac catcaccgaa gcgccggcat tgcgcgccgc
     1141 ctcgagggtg gcgggagcgt cgggcgtagc gaggtacacc gaccacatgt ccgacggcgc
     1201 tccgggctcg gcagtcatga ggccggccac ctcctcgccg tgcagccgga agttggagta
     1261 ccccccgaag tcggggttcg gctcgtcgga cgtccaaccg aagagttcgg tgtagaacgc
     1321 gacgctggcg tcgcggtccg acgtcatgag gtcgatccag atcggcactc ctgctgctgg
     1381 ctgctggcga atggtcacgc ggatgctcct gtcgatggtg ggtccgggag ggcagacgac
     1441 gtgagcggcc gattctcatc gctgcaggcg ttccgtgcac gtcgtggtgg gtatccatgt
     1501 gccccggcga catcccccgc actaccgttg cgcccatgtt gaacctggtg ctgcccaagg
     1561 gatcgctcga gaaggcgacc ttccaactct tctccgacgc ggacctcggc atccaccgca
     1621 actcggcggt cacctacaag gccacgattg ccgatccccg catcgccagc gtgcgcatcc
     1681 tgcgccccca ggagatcccg gtctacgtcg ccgacggcct gttcgacctg ggtgtgtgcg
     1741 gccgggactg ggtcgaggag acgtcgtcga acgtcgtctc gctcggcgag atgggctact
     1801 cgaaggcaac gaccaacccc atcaagatgg tggttgccgt ggcgggcgat gccgaatggg
     1861 agaaggtcgc cgacctgccc cagggggtga aggtctcgac cgagtacccc gagatcaccc
     1921 gccgcttctt tgagcagaac ggcatcgaag ccgacgttcg cctgagctac ggcgcgaccg
     1981 aggccaaggt gcccgacatc gtcgactgcg tggtcgagat caccgagacc ggcaacgcgc
     2041 tccgagccgc cggcctcaag gtcatcgaca cgatcctcca cagctacacc gaggtcgtcg
     2101 ccaatccggc gacgtacgag gtgcccgaga agcgccacgc gatggagcag atcatgaccc
     2161 tcctgcaggg tgtgctcgag gcccgcagca aggtgctggt gaagctcaac gtgtcgaccg
     2221 acgacctcga tgcggtcatc gcgaagctgc cggccatgaa gacgccgacc gtcaacgagc
     2281 tgtaccgccg caacgggtac gccgtggaga ccgtcgtccc gaagaacgag atcaacatcc
     2341 tgatccccgc actcaaggac gccggagcca ccgacatcct cgaactgcca ctcgcaaaaa
     2401 tcgtgcactg atgaccgttc gcaccggcac ggtgaccgag ttcgacgacc cgcggggcat
     2461 tggtgtcgtc acgggagcgg acggcaccga gtaccccttt cactgcacgg cgatcctcga
     2521 cggcacccgg cagatcgacg tcggagcatc ggttcgcttc gtcgaccggc ccggtcgtca
     2581 gggccgttgg gaagccaccg agatcgcccc ggtctgatcg cggcgttcga agcagctacg
     2641 gcaggagtgg ggaacacggc gtcgtagcgt cgccggtcga accgtcgggg caggtcgacc
     2701 cgacgaagct ctgcgtgcca tggaactcgg cgccggtcca gtccgacgta cggaagtcga
     2761 tgtcgtccca ctggcccgac ccgagcacgg cgaacgggcc gatcgtcgtc gaaccgaaca
     2821 cggcgccgct gaaatcggcg ttggccgccg aaccgctgag cgccgccagg tctgcggaga
     2881 cccctggcgt cggcacggcc gagctcaggt tgcgccaggt gacccgctgg ccgttcccgt
     2941 cgggttcacc gaactgcact ccccgcatcg aggccgagtc gaacacaacg agcgccggaa
     3001 gggcgctgcg gaggtcgtct ccgaggtcgg tgcaactgat gccggtgccg ccccagaacc
     3061 gggcgccgtc gagatcggca ttcgtgaggt cgagggcgca gagcttgctg ccgccgtcga
     3121 acagggcggc gccgaagtcg gcgccggtgg cgtccacgtc gatcagctgg acgccggtga
     3181 ggtgcgcccg ctcgaaccgt ccgccgctca cgtcgcggca gccgagcacc tggccgtcga
     3241 acgtggcgtc ggagaagtct gcctcggcga gggagaggcc gcgcaacgcc agtgacgaca
     3301 gcaggttgcc cggaccgccg aaggtggcgc cacggaagtc ggtcgcggcg tcggtcacga
     3361 cactgcgccg tccctcggtg gccaacgtcg acgacgaacg cgtgaggtag aagtcgggga
     3421 tgccgaaccg agcgccgtat ccctcgtcgc agatctcccg gccggtgtcg acggtcgcct
     3481 cgttgtaacc cgggcagtag gcaccgcatg ccggtggtgc cgggtctccg gccccgaatc
     3541 cgaacacggt cccgaagtcg aagctcgcgc cggtgagatt ggtgagtgcg aggatcg
//
LOCUS       DAOSOA010000332         3588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_26044_length_3588_cov_5.717521, whole genome shotgun sequence.
ACCESSION   DAOSOA010000332 DAOSOA010000000
VERSION     DAOSOA010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26044_length_3588_cov_5.717521"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PKY13_15130"
     CDS             complement(<1..1296)
                     /locus_tag="PKY13_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT81326.1"
                     /translation="MPAASSQPHRRLESPLEQIERAVQDRAKAIALDLGGPDGEAQLR
                     HLIDDEILRWQDDHKRGNRPHALVDPSTVAERAFRNIARYGPLTPLLDDDDVWEIMIN
                     SPGEIFVKRHHGLGGYHDEVFEDDDHVVRTLTKILDDSSTSHRKLDPTEGLQDAQLDT
                     GARLHIVHGDLARGGHVMVNIRKFTGVAFTTLDQLVERNMMTADVARFLAACVRARAS
                     IVFAGAPGSGKTTMLSCCAGELDPSLRVVTAEEVFEMDIPVANVAAMQTRAPRADRPA
                     IDLRRLVSGFLRMAPDVAIVGEVRDREALPLLLTLSSGVKGYTTIHAGSARQALTRLR
                     FICQLADTTSELPLSALNTLVSEAIDVVVHSERGPDGPRITSVVAVEDLIAGADASQF
                     TITEVFHRRTVRDQIAPTGSVPSRLSALFERAGLDTGDYL"
     gene            complement(1376..2122)
                     /locus_tag="PKY13_15135"
     CDS             complement(1376..2122)
                     /locus_tag="PKY13_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81327.1"
                     /translation="MTPDPGAPSGFERRLAVDGPETAPGALGVLGVVGVALLPVLLGW
                     AWISPLLPSTLPVWAGTLALVGAATAAFVGVTSLRAVAAAAPRSERVVAVVVAPDRIV
                     FETDGPNGPSAFAFDPSEIESIRRQRRLVEYRDADHARAAARWDHDASIDMAEIVVAE
                     RAGVPGVFCLRGRHATAIAIELSQLPHRPPVATRLRNGVGLPGRGFGANPAPAAMLAG
                     LGRAVPGLAAVVSAGTLCRVAALGFGLLGG"
     gene            complement(2119..3315)
                     /locus_tag="PKY13_15140"
     CDS             complement(2119..3315)
                     /locus_tag="PKY13_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81328.1"
                     /translation="MTRTNALRIAATAMAVAVSCLGCSNTDESHRPSTDSTATTTTPS
                     GPLLIAQPVGDRWRSPVADFVGRPQRFRSDHWPDNVVATIAEADGFLPTFGPLANYTV
                     DRTLGGRTLYRFPQFEGPGERRWAEADGRLWTVTIEQPGAAATPWPAAQWESELAGLF
                     EHLDYHNGRFTPGDPYVDVEPLDDSLAHWNDAHRVWQNGDDRYMVTGFDDRFLDLESV
                     SGRDVRVRGHAGRLDNERLVWREDDNTVVVLGSSIAGFDEVTAADLVGRPWGGAALDP
                     DGVVDVANRLEVGTSEEYGRIPLHSLAHDRANVVVRVTGDDGSTWVRRADGSGTVVSD
                     PAGGADGVALTLRAEDGPVRVWAAGEPYGPRDVPECAVTVDPSAGGPGAPLAVDVGAD
                     CVLPSR"
     gene            complement(3377..>3588)
                     /locus_tag="PKY13_15145"
     CDS             complement(3377..>3588)
                     /locus_tag="PKY13_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81329.1"
                     /translation="STSTSTTEPSATAPGSRPSGGTPSAVGAGNASPLAFTGGSGWLS
                     GLVALASIALGAVTLNVYRRRRRGA"
BASE COUNT          527 a         1187 c         1358 g          516 t
ORIGIN      
        1 gaggtagtcg cccgtgtcga gaccggcgcg ttcgaacagg gcggagagcc gtgacggaac
       61 ggagccggtc ggcgcgatct gatcgcggac cgtgcgccgg tggaagacct cggtgatggt
      121 gaactgcgat gcgtcagcgc cggcgatcag gtcctcgaca gcgacgaccg atgtgatgcg
      181 gggcccgtcg ggccctcgtt cggagtgcac cacgacatcg atggcttcgg acaccagtgt
      241 gttgagggcc gacagcggga gttcgctggt ggtgtcggcg agctgacaga tgaacctcag
      301 gcgagtcagt gcttggcgcg ccgagccggc gtggatcgtg gtgtacccct tcacgcccga
      361 cgacagcgtg agcagcagcg gaagggcttc gcgatcacgg acctcgccca cgatcgcaac
      421 atcaggagcc atccgcagga accccgacac cagacgtcgg aggtcgatgg ccgggcggtc
      481 ggcgcgtggc gcccgcgtct gcattgcagc gacgttcgcc acggggatgt ccatctcgaa
      541 cacctcttca gcggtgacga cgcggagtga cggatcgagc tcgccggcac agcagctcag
      601 catcgtcgtc ttgcccgagc ccggggcccc ggcgaagacg atcgacgctc gggcccgcac
      661 gcaggcagcg aggaagcggg caacgtccgc cgtcatcatg ttgcgctcga cgagttggtc
      721 gagcgtggtg aacgccacgc ccgtgaactt gcggatgttg accatcacgt gaccgccgcg
      781 ggcgaggtcg ccgtgcacga tgtggagccg ggcgccggtg tcgagttgcg cgtcctgcag
      841 cccttcggtg ggatcgagct tgcgatgtga cgtcgacgag tcgtcgagga tcttcgtgag
      901 ggtgcggacg acgtggtcgt cgtcctcgaa cacctcgtcg tgatagccgc cgaggccgtg
      961 gtgccgcttg acgaagatct cgccggggct gttgatcatg atctcccaga cgtcgtcgtc
     1021 gtcgaggagg ggagtgaggg gcccgtaccg ggcgatgttg cggaacgctc gctcggcgac
     1081 ggtgctggga tcgaccaagg cgtgggggcg gttgccgcgc ttgtggtcgt cctgccagcg
     1141 aaggatctcg tcgtcgatga ggtgtcggag ctgggcctcg ccgtcggggc cgccaaggtc
     1201 gagggcgatc gccttggcgc gatcctgaac ggcccgctcg atctgttcga gcggcgattc
     1261 gagtcggcgg tggggctgcg aggaggcggc gggcatatcc cacgttgtga cggggagatc
     1321 gcccgctgtc gagcctgtcg ggaggcattg cccagcgacc caccggctcc cgggctcagc
     1381 cgcccaggag gccgaacccc agcgctgcga cccggcagag cgtgccagcg gagacgacgg
     1441 cggcgaggcc cggcaccgct cgtccgagcc cagcgagcat cgctgccggg gcggggttcg
     1501 ctccgaatcc gcggccgggg aggccaactc cgttgcgcag tcgcgtggcc acgggcgggc
     1561 ggtgcgggag ctgggacagc tcgatggcga tcgctgtggc gtgccgaccc cggaggcaga
     1621 acaccccggg cactccggcg cgttcggcga cgacgatctc ggccatgtcg atcgaagcgt
     1681 cgtggtccca ccgtgccgcg gcgcgggcgt ggtcggcgtc gcggtactcg accagacggc
     1741 gttgacggcg gatcgactcg atctcggacg gatcaaacgc gaaggcgctc ggaccgttgg
     1801 ggccgtcggt ctcgaacacg atgcggtccg gagcgacgac cacggcgacc acgcgctcgc
     1861 tgcggggagc ggccgcggcc acggcgcgga ggctggtcac gccgacgaac gccgctgtgg
     1921 cggcgccgac cagcgcaagt gtccccgccc agaccggaag ggttgagggc agaagtggcg
     1981 agatccacgc ccacccgagc aggacgggta acagcgccac cccgacgacg ccgagcaccc
     2041 cgagcgcgcc gggcgccgtc tcggggccgt cgaccgccag gcgtcgctcg aagcccgacg
     2101 gtgcgcctgg gtcgggggtc atcgcgaggg gagcacgcag tcggcgccga cgtctacggc
     2161 gagcggtgcc cccggccctc cggccgacgg gtcgacggtc acggcgcact cgggcacgtc
     2221 gcgtgggcca tagggttcgc ccgcggccca gacgcgcacc ggaccgtcct cggctcgcag
     2281 tgtgagggcc acgccgtcgg cgccgccggc cgggtcgctc accacggtgc cggatccgtc
     2341 ggcgcggcgc acccacgtcg atccgtcgtc gcccgtgacg cgcacgacca cgtttgcccg
     2401 gtcgtgggcg agggagtgca gcgggatgcg cccgtactcc tcggacgttc cgacctcgag
     2461 acggttcgcc acgtcgacga caccgtcggg gtcgagggcg gcaccgcccc acggccgccc
     2521 gacgaggtcg gccgcggtca cctcgtcgaa cccggcgatc gagctgccga gcacgaccac
     2581 cgtgttgtcg tcctcgcgcc acaccagacg ctcgttgtcg agtcggccgg catggcctcg
     2641 cacgcgcacg tcgcggcccg acaccgactc caggtcgagg aagcggtcgt cgaagcccgt
     2701 gaccatgtac cggtcgtcgc cgttctgcca gacccggtga gcgtcgttcc agtgggcgag
     2761 gctgtcgtcg agcggctcga cgtcaacgta cggatcgccc ggggtgaacc ggccgttgtg
     2821 gtagtcgagg tgctcgaaga gtccggcgag ttccgactcc cattgcgccg cgggccaggg
     2881 cgtcgcggca gcacccggct gttcgatcgt cacggtccac agccggccgt cggcctcggc
     2941 ccaccggcgc tcaccggggc cctcgaactg ggggaagcgg tagagcgtgc ggccgccgag
     3001 agtccggtcg acggtgtagt tggcgagcgg tccgaacgtg gggaggaagc cgtcggcctc
     3061 ggcgatggtg gcgacgacgt tgtcgggcca gtggtcgctg cggaaacgct ggggtcgccc
     3121 gacgaagtcg gcgacgggac tccgccatct gtcgccgacc ggttgagcga tcagcagcgg
     3181 tcccgagggc gtcgtcgtcg tcgccgtgga atctgtgctc ggtcgatgtg attcgtcggt
     3241 gttcgagcag ccgaggcagg agacagcaac tgccatcgcc gtcgccgcga tccggagagc
     3301 gttcgtccgc gtcatctcgt cagtctccca gggagtcgac ggacgtcgac gaggtcggca
     3361 tggggagatc cccccttcag gccccgcgcc tgcggcggcg gtagacgttg agcgtgactg
     3421 cgccgagggc gatcgacgcg agcgcgacga gtcccgagag ccaaccggac cctccggtga
     3481 acgcgagcgg tgaggcgttg ccggcgccca ccgccgacgg agtcccgcca gatggcctgg
     3541 agcccggggc ggttgccgac ggctcggtgg ttgacgtcga ggtcgagg
//
LOCUS       DAOSOA010000333         3588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_26052_length_3588_cov_3.200962, whole genome shotgun sequence.
ACCESSION   DAOSOA010000333 DAOSOA010000000
VERSION     DAOSOA010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26052_length_3588_cov_3.200962"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKY13_15150"
     CDS             complement(<1..273)
                     /locus_tag="PKY13_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81330.1"
                     /translation="MSPHSANTRRRRLTALVVAGGLVLAACGSDDDPADTAAPVDTTS
                     GSTTESAGPRTKYPLTIDNCGHEATFERAPEKALLLYGASVAEVETM"
     gene            447..1235
                     /locus_tag="PKY13_15155"
     CDS             447..1235
                     /locus_tag="PKY13_15155"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOT81331.1"
                     /translation="MTARTDQQLDVAFVVAPGAELVDIAGPWGVFEYTTSPTTGRSPF
                     ALSVVAETTDPLVLSGGMRVVPEQTFTSCRTPDIVVVPAIGVEFSDGLLDWLRASHGQ
                     ASVTMSVCNGAFALASAGLLDGRTATCHHGSYRLMRSLYPTVTIERGVRWIDHGDIAT
                     AGGLTSGTDLALHIVDRLLGSGAAELAASQLEYFGDGWRDPTVNAEFAIDPVPPPGLA
                     LDPICGMAINPQGAITATWHGNDHAFCSEWCRDMFLASPERFTS"
     gene            complement(1357..1695)
                     /locus_tag="PKY13_15160"
     CDS             complement(1357..1695)
                     /locus_tag="PKY13_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81332.1"
                     /translation="MTDPDGTDSVAPGLTNPETSGSGWTDLGSPGGHDETIESAGEPQ
                     GMIPARTRRRTGFERVSMRILATGGIIGIAVALGAILVSKSVDGWIVGLVVGLTSVTL
                     AAVLWSSRQL"
     gene            2186..2389
                     /locus_tag="PKY13_15165"
     CDS             2186..2389
                     /locus_tag="PKY13_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81333.1"
                     /translation="MTNILRDITVSCPPEHVFAVLAKVERLPEFSDMTVRVENAPGRP
                     VEIGDRFDQVVRVKALCEAAPTT"
     gene            2553..2990
                     /locus_tag="PKY13_15170"
     CDS             2553..2990
                     /locus_tag="PKY13_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81334.1"
                     /translation="MALLEAAASLPIDDLAGLIEAAGREMGATSARFLVADYGLLSLR
                     PLEPAGGRQQDDATIDLTVAGRAFSRAEVLVAGTRVFIPLVEGSERLGVLELSHPDWT
                     DEVSSNLRPVVQALVLLLVSRRRYTDGLLRARRSEPLSLAAEI"
     gene            3009..>3588
                     /locus_tag="PKY13_15175"
     CDS             3009..>3588
                     /locus_tag="PKY13_15175"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOT81335.1"
                     /translation="MSCTTSRVSVSGILEPAYSIGGDSFDFAINSDVAEFAIIDAVGH
                     GMNAVLISTVAVNGLRNARREGMSLDDAYTSTGDAISTQFGGSNFVTGQFGSLDLQSG
                     EMTWLNAGHPPPLIVRDGHVMGELPCCPSMPMGLGGTVVDIAVDQLHPGDSVLFYTDG
                     VIDTKSPHGTPFGFHRLSDLLERATRQGEQPVE"
BASE COUNT          573 a         1267 c         1164 g          584 t
ORIGIN      
        1 catcgtctcc acctcggcga ccgacgcccc gtacaggagc agcgccttct cgggtgcacg
       61 ctcgaaggtc gcttcatgcc cgcagttgtc gatcgtgagc gggtacttcg tccgcggccc
      121 agccgactcc gttgtcgaac cgctcgtcgt gtccaccggc gcagcggtgt cggcgggatc
      181 gtcgtcgctg ccgcaagcgg ccagaacgag ccctccggcg acgaccagcg ctgtgaggcg
      241 gcgtcgccgg gtgttagcgg aatgagggga catgggtgac gtctttcgtc cgggaggagg
      301 tgatggtgat cgcacccgag acggggagag ccaccgagcc tttggtaagt cgccggaacg
      361 accccccgag ttcccgatcg agaaaaggtc gaccgatcgc cgagatgagc agcaactcca
      421 ccagcgaaaa cgacaagatg tcggccatga ccgctcgcac cgaccaacag ctcgacgtgg
      481 cgttcgtcgt cgcaccgggg gccgaactcg tcgacatcgc cggcccgtgg ggagtcttcg
      541 agtacacgac gtccccgacc accggtcgat cgcccttcgc tctctcggtc gtggcggaga
      601 ccaccgaccc gctcgtgctc tcgggcggga tgcgggtcgt gccggagcag acgttcacca
      661 gttgccggac ccccgacatc gtggtcgtcc ccgccatcgg cgtggagttc tccgacggct
      721 tgctcgactg gttgcgcgca tcacacggcc aggcatcggt gaccatgtcg gtgtgcaacg
      781 gggcgttcgc tcttgcttcc gccggcctgc tcgacggcag gaccgcaacc tgtcaccacg
      841 gctcgtaccg gttgatgcga tcgctctacc cgaccgtcac gatcgaacgc ggcgtgcggt
      901 ggatcgacca cggcgatatc gccacagccg ggggtctcac gtccggcacc gacctcgcgc
      961 tccacatcgt ggaccgcctg ctcggttccg gcgcagcaga actcgccgcg tcccaactgg
     1021 agtatttcgg cgacggctgg cgcgacccca ccgtcaacgc cgagttcgcc atcgaccccg
     1081 tcccgccgcc cgggctcgca ctcgacccga tctgcggcat ggccatcaac ccccaaggcg
     1141 ccatcaccgc aacgtggcac ggcaacgacc acgcgttctg cagcgagtgg tgtcgtgaca
     1201 tgttcctcgc ttcgccggag cgtttcacca gctgacgccg cctgtcggcg tctggttcgt
     1261 gccggccgac accggcggaa ccgccgacgt ggcgaacggt cctcgaggac ctggaactcc
     1321 tggtagtgcc gtcctcggat cggccgactc caagggtcag agctgtcgcg acgaccagag
     1381 gacggcagcc agggtcacgg aggtcaggcc gacgaccagc ccgacgatcc acccgtccac
     1441 gctcttcgac accaggatcg ctccgagcgc gacggcgatc ccgatgatcc cgcccgtcgc
     1501 caggattcgc atcgagaccc gctcgaaacc ggtgcggcga cgcgtgcggg cagggatcat
     1561 gccctggggt tcgcccgcgg attcgatcgt ctcgtcatgt ccgccgggcg atccgaggtc
     1621 ggtccagccc gaacccgagg tctccgggtt cgtcaacccc ggggcaactg agtccgtgcc
     1681 atctgggtct gtcattgtcg tctcctcact cggtcagctc gttcgattcg tgcggtcagt
     1741 gatcggcgga cgccgcgtcg tggatcgctg catcgcgccg aacggcagag cgtccccgga
     1801 cgcgaggatc ggcgcgtccg gcgcagagcc cgacgcgaat ggttggccag acttcggacc
     1861 aaggagcatg gcgagccctc gctgttcgga gacggagatt tcctcacgcg gccggggtcc
     1921 ggggcaacgc actcgtcggc ggctcacgcc gacgtcaggt gagcgtaccc ccagacgctc
     1981 ctcacaactg cacgcgccgg tcgagggcga tccgagggtt tctggcgggt tggacgatcc
     2041 gtccgttgcg gctcgacgca cgcatccgca tggacgactt cgggatgggc gactcgtcgc
     2101 cgatcggtgt accgagcacc caaacggggt accgccaggc cagcgtgtct gccgacgtcc
     2161 ggtctgcacc cgacctggag gccgaatgac caacatcctg cgagacatca cggtgtcgtg
     2221 cccgcccgag cacgtgttcg ccgtactcgc gaaggtcgag cggttgcccg agttctccga
     2281 catgaccgta cgcgtcgaga acgcccctgg ccggcccgtc gagatcggcg atcgattcga
     2341 tcaggtcgtt cgcgtcaagg cgctctgcga ggccgccccg acgacgtgag ccggctggaa
     2401 cgcctcgacc gccgcaccgg agcgaaccgc cgatccgaga accgcatgcg ccaccacgat
     2461 gatgtgcacc tggacgaaca ccggtcgcgc gaagcggcga tgccatgacg gcgccggaac
     2521 gaaccccagg ccctcgccgg gctgggcccg tcatggcact gctcgaagcg gcagcctcct
     2581 tgccgatcga cgacctggcc gggttgatcg aggccgcagg tcgcgagatg ggggcgacct
     2641 cggcccggtt cctcgtcgcc gactacggcc tgctctcact ccgcccgctc gagcccgccg
     2701 gcgggcgcca acaggacgat gccaccatcg acttgaccgt cgccggaagg gccttctcgc
     2761 gcgccgaagt gctggttgcc ggcaccaggg tattcatccc gctcgtcgag ggcagcgagc
     2821 ggctgggtgt gctcgagctc tcccatccgg actggaccga cgaggtgagc tccaacctcc
     2881 gcccagtcgt gcaggcactc gtcctcctcc tcgtcagccg acggcgatac accgacgggc
     2941 tgctccgggc tcgccgttcc gagccgctgt cgctcgccgc cgagatctag tgggggctcc
     3001 ttcccccgtt gagctgcacg acctcgcggg tgtcggtgag cggcatattg gagccggcct
     3061 attcgatcgg cggggattcg ttcgacttcg cgatcaactc cgacgtagcc gagttcgcga
     3121 tcatcgatgc ggtgggccac ggcatgaacg ccgtcctcat ctcgaccgtc gccgtgaacg
     3181 gactccgcaa cgctcgccga gagggcatga gcctcgacga cgcctacacc tcgacgggcg
     3241 acgcgatcag tacccagttc ggtggctcca acttcgtcac cgggcagttc ggctccctcg
     3301 atctgcagtc gggagaaatg acgtggttga acgccggtca tcccccgccc ctgatcgtcc
     3361 gcgacggcca cgtcatgggc gagctcccgt gctgtccatc gatgccgatg ggtctcgggg
     3421 gaacggtggt ggatatcgcc gtggaccagc ttcatcctgg cgacagcgtg ctcttctaca
     3481 ccgacggcgt catcgacacg aagtcaccgc acggcacgcc gttcgggttc caccgactga
     3541 gcgatctcct cgaacgtgcg acccggcaag gcgagcaacc ggtcgaga
//
LOCUS       DAOSOA010000334         3553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_26393_length_3553_cov_7.548885, whole genome shotgun sequence.
ACCESSION   DAOSOA010000334 DAOSOA010000000
VERSION     DAOSOA010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26393_length_3553_cov_7.548885"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PKY13_15180"
     CDS             complement(<1..956)
                     /locus_tag="PKY13_15180"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT81336.1"
                     /translation="MTRHVGARRARLEDRPLLTGGGCYTDDIELPRMAHAAIIRSPVA
                     NGIVRRIRSDRLTTPVDLVLGPDEIAAATDPYRVLWIMPNQWQTERPLYDGRVRFVGD
                     PIGVVVAADRYAAEDALDELDVLIDELPPVVGLDAALADERLVYPDHDTNLMAHWSAG
                     DDDAHTDAVFAAADRTLSIPLRIGRVAGVPIEPRGIVVDPGRPGWDGLPDKITVWTST
                     QAPHAVRDAIAEVCRLPQHRIRVIGPDVGGGFGVKDHIYEDELMVVLAAIRLGRPVKW
                     IEDRRESLTTTTQARDERHVVEVAYDDDGALRGIRVAADRDCG"
     gene            complement(995..1516)
                     /locus_tag="PKY13_15185"
     CDS             complement(995..1516)
                     /locus_tag="PKY13_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOT81337.1"
                     /translation="MALTAAGNTRSTTFDLNGRETTVVVQPRTLLSDTIRHTVGEHGT
                     HVGCEQGACGACTVIVDGVARRSCLTLTPQVDGCRVETIESVATPAGLDPVQQAIHDH
                     HGMQCGYCTAGIVMSLVAARRAGVPVESAIDEVLNGHLCRCTGYVNLRAAITAAWPSI
                     EAIAVDRLLGPTA"
     gene            complement(1516..2340)
                     /locus_tag="PKY13_15190"
     CDS             complement(1516..2340)
                     /locus_tag="PKY13_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOT81338.1"
                     /translation="MKPAPFAYADPGSIDEVLELLAEHGDDAVVLAGGQSLVPDLNFR
                     RIRPRLVIDLRRVAGLSGVSVDGDAVVAGAGCGVLDLAAAHPASGVAEAVSCIGHPQI
                     RCRSTVGGTVALAEPAAELPAVVLGLDGVVTVRSTTGERELSASEWFVGRRQSARRTD
                     ELVTEVRLAVPTGVSGWAEVSRRPNDLPIVGLFAARCSDGWRVALAGVAETPVRSTAC
                     EAALDVDPGGIDAAVAAMVAELDPPSDVLVSAAHRRSIAASLLRRVLAPVVTEAAA"
     gene            2430..>3553
                     /locus_tag="PKY13_15195"
     CDS             2430..>3553
                     /locus_tag="PKY13_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT81339.1"
                     /translation="MAVPHPPLLLRRVAHLATFDDAGTELSGVDVLCRNGSIEAIGVD
                     LDAPDATVIDASELLVLPGLINAHQHLYQVGLRAIPELERALIGPWLGGLGARCLDWW
                     DSGRLTPPFVEALAACGLAESLLGGVTTVADQHYLHPAGPTQPFIEATIAAARALGGR
                     LHAGRGTLTLGRKQGGSADPRLVQSSTEVVRHATELIDEFHDPEPGAMVRIDLAPCGV
                     HVDGADVFEAFAELAEDHDGVRLHTHLYEAVDTAFCVDRYGITPWQFLQRHGWASDRT
                     WVAHVVDIPLHEIAQLAEAGVGVAHLPAPDLRMGWGTAPLREFLDAGVTVGFGTTGSA
                     SNDGANLLGDLRLAALAHRHHDDPALWPTARELLWAATRGS"
BASE COUNT          510 a         1281 c         1278 g          484 t
ORIGIN      
        1 ccgcagtcac gatcggcggc cacacggatg ccccgcagcg cgccgtcgtc gtcgtacgcg
       61 acctcgacga cgtgccgctc gtcgcgggcc tgcgtcgtgg tggtgagcga ctcgcgtcgg
      121 tcctcgatcc acttcaccgg tcgtccgagg cggatcgcag cgagcacgac catcagctcg
      181 tcctcgtaga tgtggtcttt cacgccgaag ccgccaccga cgtcgggccc gatgacccgg
      241 atgcggtgct gcgggagccg gcacacctcg gcgatcgcgt cgcgcacggc gtgcggcgcc
      301 tgcgtcgacg tccacaccgt gatcttgtcg ggcagaccgt cccaacccgg gcggccgggg
      361 tcgacgacga tgccacgcgg ttcgatcgga acgcccgcca cccgcccgat gcgcaacggg
      421 atcgacagcg tgcggtcggc agcggcgaac accgcgtcgg tgtgggcgtc gtcgtcgccg
      481 gcgctccagt gcgccatcag gttggtgtcg tggtcggggt acacgaggcg ttcgtcggcc
      541 agggccgcgt cgaggccgac gaccggcggc agctcgtcga tcagcacgtc gagctcgtcg
      601 agggcgtcct ccgccgcgta gcggtcggca gcgacgacga caccgatcgg gtcaccgacg
      661 aagcggacgc gaccgtcgta caggggccgc tccgtctgcc actggttcgg catgatccac
      721 agcacccggt aggggtcggt ggcagcggcg atctcgtcgg gccccaggac gaggtcgacg
      781 ggcgtggtga gccggtcgct gcggatccgt cgcacgatcc cgttggcgac ggggctgcgg
      841 atgatcgccg cgtgggccat gcggggcagc tcgatgtcgt cggtgtagca accaccgccc
      901 gtcaacagcg ggcggtcctc gaggcgggcg cgtcgggcac cgacgtgtcg cgtcaccgac
      961 cgggccggtt ccaacatcgt cgggcgcatg cccgtcacgc ggtcggtccg agcagtcggt
     1021 ccacggcgat ggcctcgatg ctgggccacg ctgccgtgat cgcggcgcgc aggttgacgt
     1081 agccggtgca gcggcacagg tggccgttga gcacctcgtc gatcgccgac tcgaccggaa
     1141 cgccggcacg gcgagcggcg acgagcgaca tgacgatgcc ggcggtgcag tagccgcatt
     1201 gcatgccgtg gtgatcgtgg atcgcctgct gcacggggtc gaggcccgct ggcgtcgcga
     1261 ccgactcgat cgtctcgacc cggcagccgt cgacctgcgg cgtgagcgtg aggcaggagc
     1321 ggcgggcgac gccgtcgacg atcaccgtgc acgcgccgca cgcaccctgc tcacaaccga
     1381 cgtgggtgcc gtgctcgccg acggtgtggc ggatggtgtc ggacagcagg gtccggggtt
     1441 ggacgaccac ggtggtctcg cggccgttga ggtcgaacgt cgtggaccgg gtgttgccgg
     1501 cagcggtcag cgccatcagg ccgccgcctc ggtgacgacg ggggcgagca ctcgtcgcag
     1561 gagcgacgca gcaatcgatc gccggtgcgc cgccgacacc agcacgtccg acggcggatc
     1621 gagttcggcc accatcgccg ccaccgccgc gtcgatgccg ccggggtcga cgtcgagcgc
     1681 cgcctcacac gccgtcgacc ggaccggggt ctcggcgacg ccggccagcg cgacgcgcca
     1741 accgtcggag catcgcgctg cgaacagccc gacgatcggg aggtcgttgg ggcgccggga
     1801 gacctcggcc caccccgaca cgcccgtcgg cactgcgagg cggacctcgg tcaccagttc
     1861 gtcggttcgc cgggccgact gccgacgccc cacgaaccac tccgaggccg acagttcccg
     1921 ctcacctgtc gtcgacctca ccgtgacgac gccgtcgagg ccgaggacga cggcgggcaa
     1981 ctcggccgct ggctcggcga gggcgacggt gccgccgacg gtggaccggc agcggatctg
     2041 ggggtgaccg atgcacgaca ctgcctcggc cacaccgctc gccgggtgtg ccgccgccag
     2101 gtcgagcaca ccgcacccgg cgccggccac gaccgcgtcg ccgtcgaccg acacacccga
     2161 caagccggct acccgtcgaa ggtcgatgac gaggcggggc cggatgcggc ggaagttgag
     2221 gtcgggcacg aggctctgtc caccggccag cacgaccgcg tcgtcgccgt gttcggcgag
     2281 gagttcgagc acctcatcga tcgacccggg gtcggcgtag gcgaacggag cgggcttcat
     2341 cgcgccgatg atacgaacgg cctcctgcga tgtcagcggg caagccgagt gttcacaccg
     2401 ctgaaacacg agggcgacaa actcccccga tggcggtccc ccacccaccc ctcctgctcc
     2461 gtcgagtcgc ccacctcgcg acgttcgacg acgccggcac cgagttgtcg ggcgtcgacg
     2521 tgctgtgccg caacgggtcg atcgaggcca ttggcgtgga cctcgacgca cccgacgcga
     2581 cggtgatcga cgcgtcggaa ctcctggtgc tcccgggcct gatcaacgcc caccagcacc
     2641 tctaccaggt cggtctccgg gcgatccccg agctcgaacg cgcgctcatc ggcccgtggc
     2701 tcggagggct cggggcccgc tgcctcgact ggtgggactc gggacggctg acgcccccgt
     2761 tcgtcgaggc gctcgccgcc tgtggactcg ccgagtcgct cctgggcggc gtgacgacag
     2821 ttgccgatca gcactacctg cacccggcgg gaccgaccca gccgttcatc gaggcgacga
     2881 tcgccgcggc gcgggccctg ggcggacgcc tgcacgccgg ccgcggcacg ctgacgctcg
     2941 ggcgcaaaca gggtggatcg gccgacccgc gcctcgtcca gtcgagcacc gaggtggtgc
     3001 ggcacgcgac cgagctcatc gacgagttcc acgatcccga gccgggtgcg atggtgcgga
     3061 tcgacctggc gccgtgcggc gtccacgtcg acggcgccga cgtgttcgag gcgttcgccg
     3121 agctcgccga ggatcacgac ggggtccggc tgcacaccca cctgtacgaa gcggtggaca
     3181 ccgccttctg cgtcgaccgc tacgggatca cgccgtggca gttcctgcaa cgacacggct
     3241 gggcgtcgga ccgaacctgg gtggcgcacg tcgtcgacat ccccctccac gagatcgccc
     3301 agctcgccga ggccggtgtg ggtgtcgccc acctcccggc cccggacctc cggatggggt
     3361 ggggcaccgc tccactgcgt gagttcctcg acgccggtgt gacggtcgga ttcggcacca
     3421 cgggttcggc gagcaacgac ggagccaacc tcctgggcga cctccggctc gcggcactcg
     3481 cgcaccgcca ccacgacgac ccggcgctgt ggccgaccgc acgtgagctg ctgtgggccg
     3541 cgacccgggg atc
//
LOCUS       DAOSOA010000335         3550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_26430_length_3550_cov_6.616023, whole genome shotgun sequence.
ACCESSION   DAOSOA010000335 DAOSOA010000000
VERSION     DAOSOA010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26430_length_3550_cov_6.616023"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PKY13_15200"
     CDS             complement(<1..1025)
                     /locus_tag="PKY13_15200"
                     /inference="COORDINATES: protein motif:HMM:NF019941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HOT81340.1"
                     /translation="MLTGKRRIVAVLASTAVIVAGLGLAFGTVEAASSVTPKAGPSGK
                     IVHGTYQGKAQKLQTMLFQVDLNGEASFMFCIDIATYIQFGVTYDEQSWDTSKVPNLN
                     KVARVLSQTNATVTKDPIEIAAAQAAIWHFSDGFQLDVANPQNDAAVTTRYNALVADA
                     EANPVSNEPAGTLSVTPTTTSVAQGQPVFYDIATTATGALSIELSDAAVTAHPANGTT
                     CDTATNISTVTGPSRICLTSTSARTNVKMTIRTASAPVSAGRVFIRPARQKLIIGKAG
                     AAQSSETVSVSWTANGRPSVSVACPKDGVRYGQPTTFTATGTDPDGDTLAYQWVLNGQ
                     PIPGQTSP"
     gene            complement(1233..>3550)
                     /locus_tag="PKY13_15205"
     CDS             complement(1233..>3550)
                     /locus_tag="PKY13_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT81341.1"
                     /translation="ATFPGADVAGRTYTPLLPYFAGHADAFRILTDDFVTDEDGTGIV
                     HTAPGHGEDDQRVGEANGIVLVSPVDDAGRYTAEIADMVGTNVFEANPIIAKRLRDER
                     KLVRHDTIDHNYAHCWRTDTPIIYKAVSSWYVKVTDFKDRLVETNQDINWIPFHVRDG
                     QFGRWLEGSRDWSISRNRFWGAPIPVWRSDDPAYPRTDVYGSLDEIERDFGVRLDDLH
                     RPGIDELTRPNPDDPTGKSTMRRVSEVLDCWFESGAMPFAQVHYPFENKEWFDTHSPS
                     DFIVEYIAQTRGWFYTMHVLSVALFDRHAFSNVMCHGVVLDSEGRKLSKKLRNYPDPV
                     EVMETIGSDPLRWYLMSSPILRGGDLKIAKDASDMRDVVRLVVNPIWNAYSFFCRYAN
                     VDGYRATERADATGQLDRYLLAKTHALVSRVTELMDAYDVAGACHEIVAFIDALNNWY
                     IRRSRDRFWAPGSVTDGALSDDKRDAYDTLYTVLVNLLRLAAPLLPLLSDEMYRGLTS
                     GGSAGGSNSTASVHLTDWPSADTLPADDALVANMDKVRAVCSAALGLREDAKLRTRLP
                     LSRLTIAGAGADELAALTALIADEINVKAVDLRTDASSFGSWQLKPNFKLLGPVLGKA
                     LPEVTKAAKSGDWTDHGDGTVTAGGHLLTEGQFEVALQPAEGLAVASARQLDPEGGIV
                     DLGLLVSLDVEVTPELEAEGLARDVIRRVQQARKDADLDITDRIALTVTAPAATLDAI
                     RAHQAMVSDAVLATAVELAVGEALAVTVARA"
BASE COUNT          505 a         1105 c         1339 g          601 t
ORIGIN      
        1 gggctggtct ggccggggat cggctggccg ttgagcaccc actggtaggc gagggtgtcg
       61 ccgtcggggt cggtgcccgt ggcggtgaac gtggtcggct ggccgtagcg cacgccgtcc
      121 ttcggacagg cgaccgacac cgaggggcgt ccgttggccg tccagctgac cgacacggtc
      181 tcgctcgact gggcggcgcc ggccttgccg atgatcagct tctggcgggc gggtcgaatg
      241 aacacgcgcc cggcactgac gggggcggac gcggtccgga tggtcatctt cacgttcgtc
      301 cgagcgctcg tggaggtgag gcagatcctc gacgggccgg tgacggtcga aatgttggtg
      361 gcggtgtcac acgtggtgcc gttggcgggg tgcgccgtca cggcggcgtc ggacagctcg
      421 atggagagcg cgccggtggc cgtcgtcgcg atgtcgtaga agaccggctg gccctgggcg
      481 acgctcgtcg tcgtgggggt cacgctgagc gtgccggcgg gctcgttgga cacggggttc
      541 gcctcggcgt cggcgacgag cgcgttgtag cgggtcgtga cggccgcgtc gttctggggg
      601 ttggcgacgt cgagctggaa gccgtcggag aagtgccaga tcgcggcctg cgcggcggcg
      661 atctcgatcg ggtccttggt gacggtcgcg ttggtctgcg acagcacgcg ggcgaccttg
      721 ttgaggttcg gcaccttgga cgtatcccag ctctgctcgt cgtacgtgac gccgaactgg
      781 atgtaggtgg cgatgtcgat gcagaacatg aacgacgcct caccgttgag gtcgacctgg
      841 aagagcatgg tctgcagctt ctgggccttg ccctggtagg tgccgtggac gatcttgccc
      901 gacggccccg ccttcggcgt caccgacgac gccgcctcga cggtgccgaa cgccaggccc
      961 agtcccgcca cgatcactgc ggtcgacgcc agcacggcga cgatgcgtcg ttttccggtg
     1021 agcatcccac tcttcattgc agcctccatc ggcccgcgct cgacgcgggt ccggagacca
     1081 gtctggggac gtttcgtcgg ttttcctccc gggcgaattc cccaggagcc ccgaatgggt
     1141 cgaacgaacc aggcgcgtcc ccacccagca ccgcatctgt cctgcggttt cgcccctcca
     1201 ggggcggaac cgcaggacgg aacgagtgag ggtcaggcgc gggcgacggt gaccgcgagc
     1261 gcctcgccga cggcgagttc gacggcggtc gcgagcacgg cgtcgctcac catcgcctgg
     1321 tgggcgcgga tcgcgtcgag cgtggccgcc ggagcggtca cggtcagcgc gatgcggtcg
     1381 gtgatgtcga ggtcggcgtc cttgcgagcc tgttgcaccc gccggatcac gtcacgcgcc
     1441 aggccctcgg cctcgagctc gggggtgacc tccacgtcga ggctgacgag cagtccgagg
     1501 tcgacgatgc cgccctcggg gtcgagctgg cgggccgacg cgacggcgag accctcggcc
     1561 ggttgcagcg cgacctcgaa ctggccttcg gtcagcaggt gtccgccggc ggtgacggtg
     1621 ccgtcgccgt gatcggtcca gtcccccgat ttcgccgcct tggtgacctc gggcaacgcc
     1681 ttgccgagca ccggccccag caacttgaag ttgggcttga gctgccacga gccgaacgac
     1741 gatgcgtcgg tccgcaggtc gacggccttc acgttgatct cgtcggcgat gagggcggtg
     1801 agcgcggcga gttcatcggc gccggccccg gcgatcgtga ggcgcgagag gggcaggcgg
     1861 gtgcggagct tcgcgtcctc gcgcaggccg agcgccgcgg aacacacggc ccgcaccttg
     1921 tccatgttcg cgacgagggc gtcatcggcg ggcaacgtgt cggcggaggg ccagtcggtg
     1981 agatgcaccg aggcagtgct gttgctgcct ccggcagatc cgccggacgt gagtccgcgg
     2041 tacatctcgt cgctcaggag cggcagcagg ggagcggcga ggcggagcag gttgaccagg
     2101 acggtgtaga gcgtgtcgta cgcgtcgcgc ttgtcgtcgc tcagcgcccc gtcggtgacc
     2161 gaccccggcg cccagaaccg gtcgcggctg cgacggatgt accagttgtt gagtgcgtcg
     2221 atgaacgcga cgatctcgtg gcacgcgccg gcgacgtcgt aggcgtccat cagctcggtc
     2281 acacgggaga ccagcgcgtg cgtcttggcg aggaggtagc ggtcgagctg accggtggcg
     2341 tcggcccgct cggtggcgcg gtagccgtcg acgttcgcgt aacggcagaa gaacgagtac
     2401 gcgttccaga tcgggttcac gaccaggcgt acgacgtcgc gcatgtccga cgcgtccttg
     2461 gcgatcttca ggtcgccgcc gcggaggatc ggcgagctca tcaggtacca gcggagcgga
     2521 tcggagccga tcgtctccat gacctcgacg gggtcggggt agttgcggag cttcttcgac
     2581 agctttcggc cctcgctgtc gagcaccacg ccgtggcaca tgacgttgct gaaggcgtgg
     2641 cggtcgaaca gcgcgaccga cagcacgtgc atcgtgtaga accagccgcg ggtctgggcg
     2701 atgtactcga cgatgaagtc cgacgggctg tgggtgtcga accactcctt gttctcgaag
     2761 gggtagtgca cctgggcgaa gggcatcgcg cccgactcga accagcagtc gagcacttcc
     2821 gacacgcggc gcatcgtgct cttgcccgtc gggtcgtcgg ggttggggcg cgtcagctcg
     2881 tcgatgccgg ggcggtgcag gtcgtcgagg cgaacaccga agtcgcgctc gatctcgtcg
     2941 aggctgccgt acacgtcggt acgggggtag gccgggtcat cggaacgcca caccgggatg
     3001 ggggcgcccc agaaccggtt gcggctgatc gaccagtcgc gggagccctc gagccagcgg
     3061 ccgaactggc cgtcgcgcac gtggaacggg atccagttga tgtcctggtt ggtctcgacc
     3121 agacggtcct tgaagtcggt gaccttcacg taccagctcg atacggcctt gtagatgatg
     3181 ggcgtgtcgg tccgccagca atgggcgtag ttgtggtcga tcgtgtcgtg gcggacgagc
     3241 ttgcgctcgt cgcggaggcg cttggcgatg atcgggttgg cctcgaacac gttggtgccg
     3301 accatgtcgg cgatctcagc ggtgtagcgg ccggcatcgt cgacgggcga caccaacacg
     3361 atgccgttgg cctcgcccac ccgctggtcg tcctcgccgt gaccgggcgc cgtgtgcacg
     3421 atcccggtgc cgtcctcgtc ggtgacgaag tcgtcggtga ggatgcggaa cgcgtcggcg
     3481 tggccggcga agtaggggag gagcggggtg taggtgcggc cggcgacgtc ggcgccgggg
     3541 aacgtggcga
//
LOCUS       DAOSOA010000336         3540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_26526_length_3540_cov_5.991966, whole genome shotgun sequence.
ACCESSION   DAOSOA010000336 DAOSOA010000000
VERSION     DAOSOA010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26526_length_3540_cov_5.991966"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKY13_15210"
     CDS             <1..214
                     /locus_tag="PKY13_15210"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOT81342.1"
                     /translation="GVSLNVGILGLCIEAVNLGYRVVVPTDATVAVPPSYADAVLANT
                     ISQLATLTDSAAVVGSWNAVTRPGAD"
     gene            complement(211..1116)
                     /locus_tag="PKY13_15215"
     CDS             complement(211..1116)
                     /locus_tag="PKY13_15215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOT81343.1"
                     /translation="MHVMVALPTDHVEHLDEFVSGPAIAEMAAHVEVCGLGGVFVTDH
                     PAPDDRWLAGGGHHALEPTVALSFAAAATTTLRLVTNIYVLAYRPPLLAAKTLATLDV
                     LSGGRLTVGVAAGYLRSEFNALGADFDDRGARLDEAIDVVRRAWTGESVVGEGAGWTA
                     RGNTILPRPRQQPGPPIWCGGNSKAAMTRAVTSADGWLPFATPPGMSGAVRTAEIASI
                     DDLRRRLDLLDRLCDEHGRAERPTICFTPISTWAEPERLLDEIGRLTELGVDWVSLTL
                     APTPGTAPSRAGWMDSVSAVAAALA"
     gene            complement(1118..1759)
                     /locus_tag="PKY13_15220"
     CDS             complement(1118..1759)
                     /locus_tag="PKY13_15220"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81344.1"
                     /translation="MTEFYRPGPSGSRQVLVYLHGHGSAPDLVHPFFRQHHDDGWVRL
                     CPRGPLPTDGGWSWFDSGPRGVDRAGLDASVARVRTLVEATTGDLGATWSDVVVAGFS
                     QGASLALGVAAALGADGVTIGGLLVQAGFAPEVFGDEVDLGTVRARSVLVQHGETDEV
                     VPSFMAGDLGASIGASSGAVDGIEVQLLPGGHTLSPAMLDAARRWLAGEPDEA"
     gene            1929..2903
                     /locus_tag="PKY13_15225"
     CDS             1929..2903
                     /locus_tag="PKY13_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81345.1"
                     /translation="MTPPMPMFSVAPRQASPEPSVAPAADRAADPASDGSADSAHTAP
                     AASVPGAGSFDVGRPESAPVPANAGPNPDFPNNAAPGAPVPGRTDVRGPAESEIVDRP
                     SALAEVASNAPLLPARQAPTAGLALARANDGLPMVLDPFAVASRAVLVLIGSVEPAKH
                     IARTAIEMSIEHDSSAAIDVVIPRAIDLVLRRAAHLVSIGAGPKVVDHYTERLELWRR
                     LARRSGLEPAAIVLTLAPFPIPTVASMLRVSLAEVEACIDDWYAGIDPDDSRPRSIAP
                     APEPVIDLRDSARNPATPAHSPAHSAPSATPRRSHRRSGWARFRIRQA"
     gene            2995..3531
                     /locus_tag="PKY13_15230"
     CDS             2995..3531
                     /locus_tag="PKY13_15230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT81346.1"
                     /translation="MLRNRRATPRTNASEEQFQALIADHAEVIYRVAYSVVRDEHLAE
                     DIVQETIIKAWQALPSWRGDGSLRGWVLSIAHNTAVSYLRRMRDTSTEPARLPERASS
                     ADVERETDARADLDRLRLALADLDELSRSIVVMRDLDGLPYQQIAEALDVPLATVKTR
                     LLRARRELQRVVEAGVHT"
BASE COUNT          520 a         1354 c         1191 g          475 t
ORIGIN      
        1 cggggtgtcg ctcaacgtcg ggatcctcgg cctgtgcatc gaagcggtca acctcggcta
       61 ccgggtggtc gtgccgaccg atgccaccgt cgccgtcccg ccgtcgtacg ccgacgcggt
      121 cctggcgaac acgatctccc aactggcgac gctgaccgac agcgccgccg tcgtggggtc
      181 gtggaacgcc gtgacgcggc ccggcgccga ctaggccagc gcggcagcga cggcggacac
      241 cgagtccatc catcccgccc gactcggcgc ggtgcccggt gtgggtgcga gcgtcagcga
      301 gacccagtcg accccgagct cggtcagccg gccgatctcg tcgagcagtc gttccggctc
      361 ggcccacgtg ctgatgggcg tgaagcagat ggtcgggcgc tcggcccgcc cgtgctcgtc
      421 gcagagccga tcgagcaggt cgaggcggcg acgaaggtcg tcgatcgacg cgatctcggc
      481 ggtgcggacg gcgccggaca tgcccggcgg cgtcgcgaac ggcaaccagc cgtcggcgga
      541 ggtgaccgcc cgggtcatgg ccgccttcga gttgccgccg caccagatcg gcgggccggg
      601 ctgctggcgg ggccgcggca ggatcgtgtt gcctcgcgcc gtccatccgg cgccctctcc
      661 gaccaccgat tcgccggtcc aggcccggcg caccacgtcg atcgcctcgt cgaggcgtgc
      721 gccgcgatcg tcgaaatcgg cacccagggc gttgaactcg gatcgcaggt atccggcggc
      781 gacgcccacg gtgagccggc cgccgctcaa cacgtcgagt gtggccagcg tcttcgctgc
      841 caggagcggt gggcgatacg cgaggacgta gatgttggtg acgagccgca gggtcgtggt
      901 cgccgcggcg gcgaacgaga gcgcgacggt cggttcgagg gcgtggtgtc cgccgccggc
      961 gagccagcgg tcgtcggggg ccgggtggtc ggtcacgaac acgccgccga gcccgcacac
     1021 ctcgacgtgg gcagccatct cggcgatggc gggaccggac acgaactcgt cgagatgctc
     1081 cacgtggtcc gtgggaaggg cgaccatcac gtgcatgtca ggcctcgtcc ggttcgcccg
     1141 cgagccagcg tcgggcagcg tcgagcatcg ccggcgacag ggtgtggccg ccgggcagga
     1201 gctgcacctc gatgccgtcg accgctccgc tcgaagcgcc gatactcgct cccaggtcac
     1261 ccgccatgaa cgagggcacg acctcgtcgg tctccccgtg ctgcacgagc accgagcgag
     1321 cacggacggt gccgaggtcg acctcgtcgc cgaacacttc cggggcgaac ccggcctgca
     1381 cgaggaggcc accgatcgtc accccgtccg ccccgagcgc ggccgcgacc ccgagcgcca
     1441 gggatgcgcc ctgggagaac ccggcgacga cgacgtcgga ccaggtggcc cccaggtcgc
     1501 ccgtggtcgc ttcgaccagc gtccgcaccc tcgcaacgga ggcgtcgagg ccggcacggt
     1561 cgacgccgcg ggggccgctg tcgaaccacg accaaccgcc gtcggtgggc agcggtccgc
     1621 gggggcagag tcggacccac ccgtcgtcgt ggtgctggcg gaagaacggg tgcacgaggt
     1681 cgggagccga cccgtgaccg tggaggtaga cgaggacctg ccgcgacccc gacggaccgg
     1741 gcctgtaaaa ttccgtcatt ccccgcctca tcctccggtc gccgcgtcag cacatgcaac
     1801 gaaatatctg ctcttgatag gtgaattcac gtctgtaccc tgtcccagag ggcgcgacgc
     1861 cccccgacga ccgactgact tggacacgca cgccagtgac agcttcccac aacactgatt
     1921 ctccggcggt gactccgccc atgccgatgt tctcggtggc gcctcggcag gcgtcccccg
     1981 agccctcggt tgcacctgcg gccgaccgtg cggccgaccc tgcctccgac ggttcggccg
     2041 actccgcgca caccgctccg gcggcgtcgg tgcccggcgc cgggtccttc gacgtgggcc
     2101 gccccgagtc cgctccggtc ccggccaacg ccggcccgaa ccccgacttc cccaacaacg
     2161 ccgcgcccgg tgccccggtc ccgggccgca ccgacgtgcg cgggccggcc gagtccgaga
     2221 tcgtcgacag gccctccgcc ctcgccgagg ttgcgtccaa cgccccgctc ctgccggctc
     2281 ggcaggcgcc gaccgccggc ctggcgctcg cccgagccaa cgacggcctg ccgatggtgc
     2341 tcgacccgtt cgctgtcgcc agccgtgctg tcctcgtact catcggttcg gtcgaaccgg
     2401 ccaagcacat cgcgcgcacc gcgatcgaga tgtcgatcga gcacgactcg agcgctgcga
     2461 tcgacgtggt gatcccccgg gcgatcgacc tggtactccg ccgcgccgcc cacctcgtgt
     2521 cgatcggcgc cggccccaag gtcgtcgacc attacaccga acgcctcgag ctgtggcgcc
     2581 gcctcgcccg ccgctctggg ctcgaacccg ctgcgatcgt gctcaccctc gccccgttcc
     2641 cgatcccgac cgtggcatcg atgctccggg tctcgttggc cgaggtggag gcgtgcatcg
     2701 acgactggta tgccggcatc gaccccgacg acagccgccc gcgaagcatc gcgccagcac
     2761 ccgaaccggt gatcgacctg cgcgactcgg cccggaaccc ggcgaccccg gcacactccc
     2821 cggcgcattc ggcgccgtcc gccacgcccc gacggtccca ccgccgatcg ggctgggcac
     2881 gattccggat ccgccaagcc tgaccaccgc gcacgtcgga ggggccgacg ccggccgcgt
     2941 ccgcacggga ggaatccccc gcaccccgcg gcacactgac cgtgaggtct caccgtgctc
     3001 cgcaaccgcc gcgccactcc tcggaccaac gcgtcggagg agcagttcca agccctgatc
     3061 gccgaccacg ccgaggtgat ctaccgagtc gcgtactcgg tcgtgcgtga cgagcacctc
     3121 gccgaggaca tcgtccagga gacgatcatc aaggcctggc aggcccttcc cagctggcgc
     3181 ggtgacggat cgctccgcgg ctgggtgctc agcatcgccc acaacaccgc cgtgtcctac
     3241 ctgcgacgca tgcgggacac ctcgaccgaa cccgcccggt tgcccgaacg ggccagctcc
     3301 gccgacgtgg aacgcgagac cgacgcccgc gccgacctcg atcgtctccg gctcgcgctc
     3361 gccgatctcg acgagctgag ccgcagcatc gtcgtgatgc gcgacctgga cggcctcccc
     3421 taccagcaga tcgccgaggc gctcgacgtg ccgttggcca ccgtgaagac ccgactcctc
     3481 cgtgcccggc gggagttgca gcgcgtcgtc gaagcaggag tgcacacatg aacaccgagc
//
LOCUS       DAOSOA010000337         3520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_26719_length_3520_cov_6.036941, whole genome shotgun sequence.
ACCESSION   DAOSOA010000337 DAOSOA010000000
VERSION     DAOSOA010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26719_length_3520_cov_6.036941"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PKY13_15235"
     CDS             <1..1281
                     /locus_tag="PKY13_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81347.1"
                     /translation="FMAAGGRLVLGEPDRIAESDSVTEPAPATEDTAAAVVPPGTCNL
                     TGAEALPALHTGGVGGHHVHSNELSCYGDETTAIVTSATCLAGEQFTLSAPTVFDNAT
                     MGSPTDERRTVDRRGNATVAVHVLGAGAGRRIAIVTDGVRTGFGGATASPDEPACATV
                     PGEDSLWQLPSTPGAGDRTDDDQATPSSTPTKGSTDERDGDRSGDPGSGDSGDSSEGD
                     TSDRGADGSTEAGGEAGGEAGDSSEGSEGSDAGSPGSAGSGQGDSGDRQSTEAGTAKP
                     PPSIFDFLSPGLKLALAQLLAVLVWYAWRRSRRRGVVVEDETPVELTSSRRVEAVGAF
                     RRRGGESRRAGDELRQRTATVLAAELGLGRDASRTQIAEAAAARTGRPAAAVVGLLDG
                     PPVRGEDELLALASNLRQLRSDTTDALVGRTPAP"
     gene            1278..2594
                     /locus_tag="PKY13_15240"
     CDS             1278..2594
                     /locus_tag="PKY13_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT81348.1"
                     /translation="MIPRRRLLVVAVAMAVGVAFYPGDVPGGTWAGLAVLNLLLGLAA
                     LVDLRAAPAAEQLVVTRSHPRSVTLGSTATLTWSVENRTDRAVEVRLGDELAPSLGAE
                     TRTCELQIPAHSTATASTTITPRRRGRFAPTRLTIAVNGPLGLVWRQRTVDEPTTIDV
                     HPRFVSRKVAESRIRRARTLELGSRTSRGIGGGSEFEQLREYSQEDQFRHIDWGATAR
                     TGRPIVRVYQPEPNQSVIVLLDNGRLMAGNVGGVPRLEHGIDATVALGTVAVALGDRV
                     GLVTFDQRTVSVVPPQSSTKQVARLTDAMFDLFPALLESDYHEVFRSTVARFRRRSLL
                     VVVTELTGAAARASLFPSLPTLVRHHAVLVACVRDPELTAWADGPVEDVDAAFRAGAA
                     AAALRERNALLAHVRALGAGVIDAEPGDLPGQLADAYINWKIRGVR"
     gene            complement(2561..>3520)
                     /locus_tag="PKY13_15245"
     CDS             complement(2561..>3520)
                     /locus_tag="PKY13_15245"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOT81349.1"
                     /translation="RRSKLSPEQADELLELYDVVSAHLSTARTHFDDQTLNDALSQLL
                     GFARGLIYRPRRRPRNGLWTFLAVIFPAAAWQTRRFVVLAAVVMLAPAVATGAWLTDN
                     AEVRNSTIDPQLQEILADRGFEDYYSSEPAEAWAFELFTHNIEVTALAFAAGAVLVLG
                     AWVLLFQNGLAIGTTAAVMYSAGKGSLFWGLVVPHGLLELSSIFLGAGAGMAIGWAII
                     APGDRSRAEAISATGMRSAVVMLGAMLCLVVAGATEAFVTPSGLPTGARVGIGVAWFV
                     GLVAWVVVAGSRAVALGATGTFRDLTEELVPLDQRTPRIFQLM"
BASE COUNT          538 a         1365 c         1182 g          435 t
ORIGIN      
        1 ttcatggcgg ccggcgggcg cctggtactc ggcgaaccgg accgcatcgc cgaatccgac
       61 agcgtcaccg aacccgcgcc cgccaccgag gacaccgccg ctgcggtcgt gccgcccggc
      121 acgtgcaacc tcaccggcgc cgaggcactg ccggcgctgc acaccggcgg agtgggcgga
      181 caccacgtcc actcgaacga gctgtcgtgc tacggcgacg agaccaccgc aatcgtcacg
      241 agcgccacct gtctcgccgg cgagcagttc acgctcagcg cccccaccgt cttcgacaac
      301 gccaccatgg gctcaccgac cgacgagcga cgcaccgtcg accggcgagg gaacgccacc
      361 gtcgccgtcc acgtgctcgg agcgggtgcc ggacggcgga tcgcgatcgt caccgacggc
      421 gtgaggaccg gcttcggcgg agcgaccgcg tcgcccgacg agccggcgtg cgcgaccgtc
      481 cccggtgagg actcgttgtg gcaactgcca tcgactcccg gcgccggcga tcggaccgac
      541 gacgaccagg cgaccccgtc gagcacaccc acgaagggca gcaccgacga gcgcgacggc
      601 gaccgctcgg gcgaccccgg ttccggcgat tccggggatt cctccgaggg cgacacgagc
      661 gaccgcggtg ccgacggatc caccgaggcc ggcggcgagg ccggcggtga ggccggcgac
      721 tcctccgagg gctccgaggg ctccgacgcc ggatcacccg ggagcgccgg ctccggccag
      781 ggcgacagcg gtgaccgaca gtcgaccgag gccggcacgg caaagccgcc gccgtcgatc
      841 ttcgacttcc tgtccccggg cctcaagctg gccctggcgc agctgctggc ggtgctcgtc
      901 tggtacgcgt ggcgccgttc gcgtcgacgg ggagttgtcg tcgaggacga gacgcccgtc
      961 gagttgacct cgtcgcgtcg ggtcgaagcc gtcggcgcgt tccgtcggcg cggaggcgag
     1021 agccgccgcg ccggtgacga gctgcggcag cgcaccgcca ccgtcctcgc cgccgagctc
     1081 ggactcggcc gggacgccag tcggacgcag atcgccgagg ccgcggcggc gcgcaccgga
     1141 cggcccgccg ccgccgtcgt cggcctgctc gacgggccac cggtacgcgg cgaggacgag
     1201 ctgctggcgc tcgcgtcgaa cctccgacag ctccgctccg acaccaccga cgccctcgtc
     1261 ggacggacac cagcaccgtg atcccccgtc gacggttgct ggtcgtcgcg gtggcgatgg
     1321 ccgtcggggt cgccttctac cccggcgacg tgccgggcgg cacgtgggcc gggttggccg
     1381 tgctcaacct cctgctcggt ctggccgcgc tcgtcgatct ccgggccgca cccgccgctg
     1441 agcagctcgt ggtcacgcgg tcccaccccc gatccgtgac cctcggctcg acggcgacgc
     1501 tcacctggag cgtcgagaac cgcaccgatc gcgccgtcga ggtacggctg ggcgacgagc
     1561 tggcgccgag cctcggggcc gagacccgga cctgtgagct gcagatcccg gcccacagca
     1621 ccgcgaccgc gtcgacgacg atcacgccgc gtcgacgcgg ccggttcgct cccacgcgac
     1681 tgacgatcgc cgtcaacgga ccgctcggcc tcgtctggcg ccaacgaacc gtcgacgagc
     1741 cgaccaccat cgacgtgcac ccccgcttcg tgagccgcaa ggtcgccgag tcgcggatcc
     1801 gtcgggcgcg aaccctcgag ctcggcagcc gcacctcgcg cggcatcggg ggcggcagcg
     1861 agttcgagca gctgcgggag tacagccagg aggaccagtt ccgccacatc gactggggtg
     1921 ccaccgcccg cacgggccga ccgatcgtcc gcgtttacca acccgaaccg aaccagtccg
     1981 tcatcgtcct gctcgacaac gggcgcctca tggccggcaa cgtcggaggc gtcccccgcc
     2041 tcgagcacgg catcgacgcc accgtcgccc tcggcaccgt ggcggtggca ctcggtgacc
     2101 gggtgggcct cgtgaccttc gaccaacgga ccgtttcggt ggtgccaccg cagtcgtcga
     2161 cgaagcaggt ggcccggctc accgacgcga tgttcgacct cttccccgcc ctgctcgaga
     2221 gcgactacca cgaggtgttc cgctcgacgg tcgcccgatt ccggcggcgc tcgctcctcg
     2281 ttgtggtcac cgaactcacc ggcgcagccg cccgggcctc gctgttcccg tcgctcccca
     2341 cactggtgcg ccatcacgcc gtgctcgtcg cctgcgtgcg cgatcccgaa ctcaccgcct
     2401 gggccgacgg cccggtcgag gacgtcgacg ccgcgttccg tgccggcgcg gccgccgcgg
     2461 cactccgcga acgcaacgca ctcctcgccc acgtccgagc gctcggcgcc ggcgtgatcg
     2521 acgccgaacc cggcgacctg ccgggccaac tcgccgacgc ctacatcaac tggaagatcc
     2581 gcggcgttcg ctgatcgagt ggcaccagct cctcggtcag gtctcggaac gtccccgtcg
     2641 cacccaacgc gacggcccgg gaaccggcga cgacgaccca cgccaccagg cccacgaacc
     2701 aggcgacccc gatgccgacc cgggcgccgg tcggcaaccc cgacggcgtg acgaacgcct
     2761 cggtcgcgcc cgccaccacc agacacagca tcgcccccag catcacgacc gccgatcgca
     2821 tgccggtcgc ggagatcgcc tccgcccgcg accgatcccc gggagcgatg atcgcccacc
     2881 cgatcgccat gccggcaccc gcaccgagga agatcgagct gagctccaac aagccgtggg
     2941 gaacgacgag cccccagaac agcgatccct tgccggccga gtacatgacc gcggcggtgg
     3001 tcccgatcgc cagaccgttc tggaagagca gcacccacgc cccgagcacc aacaccgcac
     3061 ctgccgcgaa cgccagcgcc gtgacctcga tgttgtgggt gaacagctcg aaggcccacg
     3121 cctcggccgg ctcgctcgag tagtagtcct cgaagccacg gtcggccagg atctcctgca
     3181 gctgcggatc gatcgtcgag ttgcgcacct cggcgttgtc ggtgagccac gccccggtgg
     3241 cgacggccgg cgccaacatg acaacggcgg cgaggaccac gaaccgacgc gtctgccacg
     3301 cggccgcagg gaagatgacg gcgaggaacg tccagagccc gttgcggggc cgtcgacgcg
     3361 gccggtagat gagcccccgg gcaaagccga gcagctggct caatgcgtcg ttgagcgtct
     3421 gatcgtcgaa gtgggtgcgg gccgtcgaca ggtgggcgct gaccacgtcg tacagttcga
     3481 gcagctcgtc ggcctgctcc gggctcagct tcgaccgccg
//
LOCUS       DAOSOA010000338         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_26753_length_3516_cov_5.370124, whole genome shotgun sequence.
ACCESSION   DAOSOA010000338 DAOSOA010000000
VERSION     DAOSOA010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26753_length_3516_cov_5.370124"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PKY13_15250"
     CDS             complement(<1..587)
                     /locus_tag="PKY13_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81350.1"
                     /translation="MRSTVVRPTAFGVTATAVAVAVFAARSSINEPALAGLVWTVFVG
                     LVVVGAVWPQVVVRFVRIEIVDVPTDAVAGIAFGLGVRVRTPGLVDTLALRIDGTEPW
                     WSVPSGAAAVELTLGRSVVQSLVVTMSTDGPLGMLSVSRRVEVPLAVPLFVGPDPVHR
                     PWTPPVAPETGSDEAAGAAAHGGDEVRSVRPYRSG"
     gene            complement(620..1609)
                     /locus_tag="PKY13_15255"
     CDS             complement(620..1609)
                     /locus_tag="PKY13_15255"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT81351.1"
                     /translation="MSDLAPDASSTAPPAGFAPHRSVTELASALFGSVSSTIKGKPDG
                     VRLALTTLLSGGHLLLQDLPGTGKTLLAKSLAVAIGGEFGRVQCTPDLLPSDITGTSV
                     FQPQQGTWQFRPGPLFADVVLIDEVNRASPRTQSALLEPMEERQVSVDGATYRLPPAF
                     FCIATQNPSGQLGTFPLPESQLDRFAMTVSLGLPDRDAEREIITGRGGIDQLPAVRPV
                     TTPAEVATAIAAVHHLHCAESVVEYVLDLVAATRTDPVIATGVSPRVSTSLLGMARAH
                     AVLSGRDYVTPDDVQYVFVPGCAHRVSLHQGVDVEAAWPVLLGILGRVPVPRS"
     gene            complement(1681..2400)
                     /locus_tag="PKY13_15260"
     CDS             complement(1681..2400)
                     /locus_tag="PKY13_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81352.1"
                     /translation="MLPTAAPNLAGWGAAPVPPGRGAGPSPVPAPIAPAPVPSAPTRR
                     RRSVARLLLPVLVVVALGAAAITWWSTRSDGNGTWETVTTASGRFRVELPETPVRTTE
                     PVDVTGRPLVVDLLRTGDLSVDEVAAGTMFVEVDLSSDPMIAQLATDPALAPALLYAQ
                     AGTVFSTAGMELGPVEPATSSLGPAIGLSGTVPGSGLVARGWMVGTEGAVLAVLVIEP
                     DGGAATATSVRDRIVASVAPV"
     gene            complement(2554..>3516)
                     /locus_tag="PKY13_15265"
     CDS             complement(2554..>3516)
                     /locus_tag="PKY13_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81353.1"
                     /translation="LGLLAEASGRMGRTDDPSALVDLLERQRANGSIAAADPVAATLQ
                     LLRACSAWWAGGADRSFCELYLGPMADAVRWLDKRKRRDAVASAIGARGALLGAAATF
                     AELGEPDVAAAVGAAADRLGAFAPELPTAAGLLERLADGLAWTEVPADGSDSAPDVGA
                     SARFVVATTNEAVRDDGDALAILAGWDVRLAGQPFEAHDLPTRAGLLSYGLRWHGERP
                     ALLWEITPWPDRPAATAVTLTAPALDPAWSATSAVGEALLAAPAGASRIIADNAERAE
                     AAARLLPDDPTQQPFQAFHHDNTPDAPVVPSPSDDPLPPGGSFS"
BASE COUNT          538 a         1253 c         1328 g          397 t
ORIGIN      
        1 tcgcccgacc ggtacgggcg caccgaccgc acctcgtcac cgccgtgggc ggccgcaccg
       61 gccgcctcgt cgctccccgt ctccggcgcg accggtgggg tccacgggcg atgcaccggg
      121 tcggggccca cgaagagcgg gacggcgagt ggcacctcga cccggcgtga caccgacaac
      181 atgccgaggg gtccgtcggt cgacatggtc accaccagcg actggacgac ggagcgaccc
      241 agcgtcagct cgaccgctgc ggcccccgac ggcaccgacc accacggctc ggtcccgtcg
      301 atccgcaacg ccagggtgtc gaccaggccg ggtgtccgaa cccggactcc cagaccgaac
      361 gcaatgccgg cgacggcgtc ggtcggaacg tcgacgatct cgatccgcac gaaccgcacc
      421 acgacctgcg gccacaccgc ccccacgacg acgagcccga cgaacaccgt ccacacgagc
      481 cccgccaacg ccggctcgtt gatgctcgac cgggcggcga acacggcgac cgcgaccgcc
      541 gtcgccgtca ccccgaaggc cgtgggccgc accaccgtgg accgcacgac ggtcgaccgt
      601 ccggcccgag cggcgcggtt cacgaacgtg gcaccgggac ccggcccagg atgccgagga
      661 ggacgggcca cgccgcctcg acgtcgacgc cctggtggag cgagacgcgg tgcgcgcacc
      721 cgggtacgaa cacgtactgc acgtcatcgg gagtgacgta gtcgcgtccc gacagcacgg
      781 cgtgggctcg ggccatgccc aacaggctgg tcgacacccg cggcgacacc ccggtggcga
      841 tcacgggatc ggtccgagtg gcggcgacga ggtcgaggac gtactcgacg accgactcgg
      901 cgcagtggag gtggtgcacg gcggcgatcg cggtggcgac ctcggcaggc gtggtgacgg
      961 gccgcaccgc tgggagctgg tcgatgccac cacggccggt gatgatctcg cgctcggcgt
     1021 cacgatcggg gaggccgagc gacaccgtca tcgcgaaccg gtcgagttgg ctctcgggca
     1081 gcgggaacgt gccgagctgg cccgacggat tctgggtggc gatgcagaag aatgccggcg
     1141 gcaggcgata ggtcgcgccg tcgacgctca cctgccgctc ctccatcggc tcgaggagcg
     1201 ccgactgggt tcgtggcgac gcacggttga cctcgtcgat caggacgacg tcggcgaaca
     1261 acgggcccgg ccggaactgc caggtgccct gctgcggttg gaacaccgac gtgccggtga
     1321 tgtccgacgg cagcaggtcg ggggtgcact gcacccgacc gaactcgccg ccgatggcga
     1381 cggcgagcga cttcgcgagg agcgtcttgc ccgtacccgg caggtcctgc aacaacaggt
     1441 ggccgccgct caagagcgtg gtgagggcaa ggcgtacacc atcgggcttg cccttgatgg
     1501 tgctcgacac cgacccgaac agcgccgacg cgagttcggt cacggatcgg tggggcgcga
     1561 acccagcggg cggcgcagtc gaggaggcgt cgggcgcgag atcgctcatg ggcgttcagc
     1621 ttggcacggc ctcggcgacg cgccgaggcg actcggggtc ggaccgaggc gaactcgggg
     1681 tcagaccggg gcgacggacg cgacgatccg atcgcgcacc gacgtcgcag tcgcggcccc
     1741 accgtcgggt tcgatcacga gcacggcgag caccgcgccc tcggtgccga ccatccagcc
     1801 ccgggccacg aggcctgagc ccggcaccgt gccgctgagg ccgatggccg gaccgagcga
     1861 tgaggtggcc ggctccaccg gtccgagttc catgccggcg gtcgagaaca ccgtcccggc
     1921 ctgggcgtac aggagtgccg gagcgagggc cgggtccgtg gcgagctggg cgatcatcgg
     1981 gtcggagctg aggtcgacct cgacgaacat cgtgcccgcg gccacctcgt cgacgctcag
     2041 atccccggtg cgcaggagat cgaccacgag aggacgcccg gtcacgtcga ccggttcggt
     2101 cgtgcggacc ggggtctcgg ggagctcgac gcggaaccgg cccgacgccg tggtcaccgt
     2161 ctcccacgtg ccgttcccgt cggaccgggt cgaccaccac gtgatggccg cagcgccgag
     2221 cgccaccacc acgaggaccg gcaggagcag tcgggcgaca gaacggcgac gccgcgtcgg
     2281 cgccgaggga accggcgctg gtgcgatggg agccgggacg ggcgatggac cggcgccccg
     2341 ccccggcggg accggcgcgg cgccccatcc cgccaggttc ggcgcggcgg tggggagcac
     2401 gggtggaccg aaccgggacg ggggcgcgga gacgatcgcg gtcccgcaca gtgggcacga
     2461 ggtgaccgac ggttccaccg ccgcgtgaca ggtcgggcac gacaccagct ccacgtgacg
     2521 acctccactc cgccggcggg ccgagcccga tgctcagctg aagctaccgc ccgggggcag
     2581 cggatcgtcg gagggcgacg gcacgacggg ggcatcgggg gtgttgtcgt ggtggaacgc
     2641 ctggaacggc tgctgggtcg ggtcgtcggg caacaggcgc gccgccgcct cggcccgctc
     2701 agcgttgtcc gcgataatgc gactcgcgcc agcgggcgcc gccaggagcg cctcgccgac
     2761 cgccgacgtt gccgaccatg ccgggtcgag cgccggggcc gtcagggtga cggcggtggc
     2821 ggcgggacga tccggccagg gcgtgatctc ccacagcagg gcgggacgtt cgccgtgcca
     2881 ccgcagtccg taggacagga gtccggcgcg ggtcgggagg tcgtgggcct cgaagggctg
     2941 tccggcgagg cgcacatccc atccggccag gatcgcgagg gcgtcgccat cgtcacggac
     3001 ggcctcgttc gtcgtcgcga cgacgaaacg ggcgctggcc ccgacgtcgg gcgcactgtc
     3061 ggagccgtcg gcggggacct cggtccaggc gaggccgtcg gccaaccgtt cgaggagacc
     3121 ggccgccgtc ggcagttcgg gagcgaacgc tcccaatcgg tcggcggcag cgccgaccgc
     3181 ggcggccacg tcgggttcac ccagttcggc gaaggtcgcc gcggcgccga ggagagcgcc
     3241 gcgcgccccg atcgcggacg ccaccgcgtc acggcgcttc cgcttgtcga gccaccggac
     3301 ggcgtccgcc atgggcccga ggtacagctc gcagaacgag cggtcggcgc cgcctgccca
     3361 ccatgcgctg cacgcccgga gcagttgcag cgtggcggcg acagggtcgg cggcggcgat
     3421 cgagccgttc gcccgctgcc gctcgagcag gtccaccagg gcggacgggt catcggtccg
     3481 gcccatccgg cccgaggcct cggcgaggag accgag
//
LOCUS       DAOSOA010000339         3498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_26945_length_3498_cov_6.587569, whole genome shotgun sequence.
ACCESSION   DAOSOA010000339 DAOSOA010000000
VERSION     DAOSOA010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26945_length_3498_cov_6.587569"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..795
                     /locus_tag="PKY13_15270"
     CDS             136..795
                     /locus_tag="PKY13_15270"
                     /inference="COORDINATES: protein motif:HMM:NF037271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT81354.1"
                     /translation="MRRSVGVDVDALLASITRPLDIVGGAGDETADRILDAAAELFAT
                     LGVRRTGMDDIAERSGLGRTTVYRRFDGRQQIVEAVLAREIHRFFGSILRSTAHIERF
                     EDVVIESFLTGLRATEASLLATLVRAEPELLQLLTIDAGPVVAAAREFLVSAYRATHT
                     EGVTASDGDLQQVAMFAEVLVRLAISLVLTPQTIIPLDDDAATRAALHRLLDPVIAPL
                     A"
     gene            complement(799..1578)
                     /gene="fetB"
                     /locus_tag="PKY13_15275"
     CDS             complement(799..1578)
                     /gene="fetB"
                     /locus_tag="PKY13_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HOT81355.1"
                     /translation="MNGDVGFVGLAVSTVMVGVAVAVSLVRRLGLERSILWACARALV
                     QLLLVGAALGLVIDPSRPAIWSWLWVAMMILFAAWTTARRAREVPGAFRLSLTAYALA
                     TGASLAVVLGLGVFPTNGRTIVPIAGMIVGNSLSATVLVGRRIVAEFADKVLEIEARL
                     ALGLTNAAAASPYLREAARTALVPQIETTKATGIVFLPGAMVGLILAGVDPIDAVRVQ
                     AAVMFLILGSVATTTSVVAVGLARRLITPDGRVVRLTRSGG"
     gene            complement(1589..2233)
                     /locus_tag="PKY13_15280"
     CDS             complement(1589..2233)
                     /locus_tag="PKY13_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT81356.1"
                     /translation="MSTPLFAFDDVGLEVDGAVILDAITVELPADGVVVVAGASGSGK
                     SSLLRLCNRLVAPTTGTVRFRGDDVAALDVRRLRRRVGMVFQRPTPFPGTVHENLRVA
                     EPSLDEDGAIALLASVGLPAEFVERDALTLSGGEAQRMCTARTLATRPEAVLMDEPTS
                     ALDEDNARGVEELARSLVGAGVSIVWVTHDRAQIDRIADVVIRLDGGRIVSGES"
     gene            2275..2952
                     /locus_tag="PKY13_15285"
     CDS             2275..2952
                     /locus_tag="PKY13_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HOT81357.1"
                     /translation="MTTARPDLAIDRAAVVERVHLDEHSWVDVARGFVLDADAMLDHV
                     LGAWDWSENRNWRYEVYVTEPRMSARVRAADLPAGIRQAGLHLRSKYHVPFDGPAPQR
                     YRHGNDSVGFHRDRSMRYLDDTRIAIVALGEPRPFLLRPYTVGYEDSSRDLDVRPGHG
                     DLIVMGGRCQADWMHAVPKVPSATERVSFTWRWTSRTGQPDSSEGYGAARNFGDSSRV
                     GPQQRRR"
     gene            2970..3482
                     /locus_tag="PKY13_15290"
     CDS             2970..3482
                     /locus_tag="PKY13_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT81358.1"
                     /translation="MSHSIDAAELADALDVIAIGRLQARYGDIVSRQAWGELDDIFLP
                     DCPISLDLRRGEPMQLVGATALAEMVAGAIERFEFFEFALLNSVIDVIDPDQGESRTA
                     TGRLYMWELRQLSTGRWTNAYGLYCDTYVRHDGRWVIGARTYSSLARTADAADGPGGH
                     DYEVFPIPGI"
BASE COUNT          578 a         1270 c         1165 g          485 t
ORIGIN      
        1 acacgctcca gctcaaggcg ttggagttgt gggtccgaca gaactggcgg ttcgtgcccc
       61 gaccgctgcg gtgggcaccc cgcgccgccg acgggtggaa gcgcgaggcg gcgctgcgca
      121 agggccaggt cgcccgtgcg tcgtagcgtc ggcgtcgacg tcgacgccct gctcgcatcg
      181 atcacccggc cgctcgacat cgtcggcggc gcgggcgacg agacggccga ccggattctc
      241 gacgctgcgg ccgagctgtt cgcgacgctc ggagtgcggc gcacgggcat ggacgacatc
      301 gcggagcgga gcggcctcgg ccgaaccacc gtgtaccggc gcttcgacgg gcgccagcag
      361 atcgtcgaag cggtgctggc gcgcgagatc caccggttct tcggttcgat cctccgctcg
      421 accgcgcaca tcgagcggtt cgaggacgtg gtgatcgagt cgttcctcac cggcctgcgc
      481 gccaccgagg cctcgctgct cgcaaccctc gtgcgggccg agccggagct gctccaactc
      541 ctcacgatcg acgccggccc ggtcgtcgcc gccgcccgcg agttcctcgt gtcggcctac
      601 cgggccaccc acaccgaggg agtgacggcg agcgacggcg accttcagca ggtcgccatg
      661 ttcgccgagg tgctcgtgcg gctcgccatc tcgctggtgc tcacgccgca gacgatcatc
      721 cccctcgacg acgacgcagc gacccgggcc gccctgcatc ggctgctcga cccggtgatc
      781 gcccccttgg cctgagcctc acccaccgga ccgggtcagt ctcacgacac gaccatccgg
      841 cgtgatcaaa cggcgggcca gtccgacggc cacgaccgac gtggtcgtgg cgacggatcc
      901 caagatgagg aacatgaccg ccgcctgcac cctcacggca tcgatcggat cgaccccggc
      961 gaggatcaga cccaccatcg cgcccggcag gaacacgatg ccggtcgcct tggtcgtctc
     1021 gatctgcggg acgagcgccg tccgcgccgc ctcccgcagg tagggcgacg ccgccgcggc
     1081 gttggtcaag cccaacgcca gccgtgcctc gatctcgagc accttgtcgg cgaactcggc
     1141 cacgatccgc cgaccgacga ggacggtcgc cgacagcgag ttgccgacga tcatcccggc
     1201 gatgggaacg atcgtgcggc cgttcgtcgg gaacacaccc aagccgagca ccacggcgag
     1261 cgacgcaccg gtcgccagcg cataggcggt cagcgacaac cggaacgctc ccgggacctc
     1321 acgcgcccgc cgagcggtgg tccacgctgc gaacaggatc atcatcgcaa cccagagcca
     1381 cgaccagatc gccggtcggc tcgggtcgat cacgaggccg agggcggcgc ccaccaacag
     1441 gagttggacg agggcgcggg cgcacgccca cagaatcgac cgttccaggc cgagccgacg
     1501 aacgagcgac accgcgaccg cgacaccgac catgacggtc gacaccgcga ggccgacgaa
     1561 cccgacatcg ccgttcacga tccgcccctc atgattcgcc gctcacgatc cgaccgccgt
     1621 ccagccggat cacgacgtcg gcgatgcgat cgatctgggc gcggtcgtgg gtgacccaca
     1681 cgatcgagac accggcgccg acgagcgacc gcgcgagttc ctcgacgccc cgagcgttgt
     1741 cctcgtcgag tgccgaggtc ggttcgtcca tgagcacggc ctcggggcgc gtcgccaacg
     1801 tcctggcagt gcacatgcgt tgggcctcac ctcccgacag ggtgagcgca tcgcgttcga
     1861 caaactccgc cgggagcccg accgacgcga ggagcgcgat cgcgccgtcc tcgtcgagcg
     1921 acggctcggc gacacggagg ttctcgtgca ccgtgccggg aaacggcgtg gggcgttgga
     1981 acaccatgcc gacgcgacgg cggagacgtc gcacgtcgag ggctgcgacg tcgtcgccgc
     2041 ggaatcgcac cgtgccggtg gttggagcta cgagccggtt gcagagacgc agcaacgacg
     2101 acttgcccga gcccgacgcg ccggcaacga ccacgacgcc gtcggcaggc agctcgaccg
     2161 tgatggcgtc gaggatcacg gcgccgtcga cctcgagacc gacatcgtcg aaggcgaaca
     2221 gcggggtcga caccgcgaca ttctcgcgct cggatctctg gcacgattcg ttcgatgacc
     2281 acggcacgac ccgatctcgc catcgaccgc gcggcggtcg tcgagcgcgt gcacctcgac
     2341 gagcactcgt gggtcgacgt cgcccgcgga ttcgtcctcg atgccgacgc gatgctcgac
     2401 cacgtgctcg gggcctggga ctggtcggag aaccggaact ggcgctacga ggtgtacgtc
     2461 accgagcccc ggatgtcggc acgggtccgc gccgccgacc tgcccgccgg gatccgacag
     2521 gccgggttgc acctccgctc gaagtaccac gtgccgttcg acggtccggc gccccagcgc
     2581 taccgacacg gcaacgactc cgtcggattc catcgtgacc ggtcgatgcg ctacctcgac
     2641 gacaccagga tcgccatcgt ggcgctgggt gaaccacggc cgttcctgtt gcggccgtac
     2701 acggtcggct acgaggacag ctcccgcgac ctcgacgtcc gtcccggtca cggcgacctc
     2761 atcgtgatgg gcggccgctg ccaagccgac tggatgcacg cggtgccgaa ggttccgagc
     2821 gcgaccgaac gggtgtcgtt cacctggcgg tggacctcac gcacgggtca acccgacagc
     2881 tccgaagggt acggcgcagc gcgcaacttc ggcgacagct cccgggtcgg gccacaacaa
     2941 cgtcggcgtt gatgggcaag gatgtcccca tgagccattc gatcgacgcc gccgaacttg
     3001 ccgatgccct cgacgtgatc gcgatcgggc gactgcaggc acggtacggc gacatcgtct
     3061 cccggcaggc ctggggcgaa ctcgacgaca tcttcctccc cgactgcccg atcagcctcg
     3121 acctccgacg cggcgaaccg atgcagctcg tcggtgcgac ggcgcttgcg gagatggtgg
     3181 ccggagcgat cgaacggttc gagttcttcg agttcgcgct gctcaactcg gtgatcgacg
     3241 tgatcgaccc cgatcagggc gagagccgga ccgcgaccgg acgcctctac atgtgggagc
     3301 tgcgccagct ctccaccggc cggtggacca acgcgtacgg cctgtactgc gacacctatg
     3361 tacgccacga cgggcgttgg gtcattggcg cccgtaccta cagctcgttg gcacgaaccg
     3421 ccgacgccgc cgacggcccc ggcgggcacg actacgaggt gttccccatt ccgggcatct
     3481 gagcgccccc gagcgttc
//
LOCUS       DAOSOA010000340         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_27050_length_3487_cov_7.173368, whole genome shotgun sequence.
ACCESSION   DAOSOA010000340 DAOSOA010000000
VERSION     DAOSOA010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27050_length_3487_cov_7.173368"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKY13_15295"
     CDS             complement(<1..233)
                     /locus_tag="PKY13_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81359.1"
                     /translation="MTRTSTDGAPNPAAGSRRRRTLLIAGVVWLALGIVLLVVIGGAT
                     GFIPDDPVVPRIGFIDAGDGRTAIVVGATSFAQT"
     gene            complement(239..1495)
                     /locus_tag="PKY13_15300"
     CDS             complement(239..1495)
                     /locus_tag="PKY13_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT81360.1"
                     /translation="MSNTLAVDYVTGFQRHTRELLARLGRLEGPLTFVPVRWCAECAT
                     FRRLTVAERAALAVFTPRDAPQRSRLAQLTDPLPETLKLRGRALIRTRIAHAAREELA
                     RRRRIRNHPPGHAELRIDEWPADSWFFDLEAAWHNVPRRDVVLPWLRDRGVHTSTLVA
                     DVMPTQFPQWFDAGQIRLFSTFIEAHLRHSEKFVCISECSRRDVEALAERLGIAADLD
                     TSVITMGANFQRADDHLPLPAEAPTGRYLLSVATVEPRKNHRLLIDAFDRLKERHPDL
                     GLVMVGKAGWMTDDLQELMRSHVDQGGRFRWLDKVDDRLLDGLYRHAFLAVQPAFYEG
                     FGTPVIEALGNGVPTLSSTGGALPEAGGEWAEYFDPTDVDELVELIERHLTDVEYHRE
                     RRDALATYRPPSWEDGAAGIEEAFRP"
     gene            complement(1570..2763)
                     /locus_tag="PKY13_15305"
     CDS             complement(1570..2763)
                     /locus_tag="PKY13_15305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT81361.1"
                     /translation="MSTYPLHPRQSGGQLRGWHLAEALGHDGLTDVSVVSLTTDPSQA
                     GRHELAPGLVEICVALPPAHAARETDLRLVTGNVSLTDIAAGLMWSGLDEFVEALGEE
                     LDGASAAVLVQPYLVDAVRAFAGDIPVICDEHNDELALKGSIIPDNAAGRWLLGHVDR
                     IERTAVEHAALVTATTDLDLDTLGARYRIAAPTTVVPNGVDTTEIEFVAGGDRRRRRL
                     ALAQDVELDPRRPAALFVGSGHGPNIDAGRVIIEVAREVPDIDFLLAGRHSSLLNVRH
                     LPPNVRVLGPVSDDLLDLLLSACDLALNPMSAGSGSNLKLLTYLAAGLPVVSTIVGAR
                     GIDADAAGVATVPLAELAAGIRDVVRQDNTDRSLAGRHYVEEHCDWRAIGRRFAALTA
                     EHIDS"
     gene            complement(2975..>3487)
                     /locus_tag="PKY13_15310"
     CDS             complement(2975..>3487)
                     /locus_tag="PKY13_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT81362.1"
                     /translation="AAGEVVIGSFSKYFSMTGWRLGWMIVPPELVEPVDRLSQNLYLS
                     PPTLAQHAALAAFDDLAELDGHVERYAANRAALLDALERMGVTELAPCDGAFYLYGNV
                     ARWGIDSRELVDQWLHELGVATAPGVDFDPVDGHRWIRWSFAGSTADVVEAGRRLVEW
                     AERIGDGSGA"
BASE COUNT          522 a         1128 c         1328 g          509 t
ORIGIN      
        1 gtctgtgcga aggacgtcgc tccgaccacg atggccgtac gtccgtcgcc ggcgtcgatg
       61 aatccgatgc gtgggacgac cgggtcgtcg gggatgaacc cggtcgctcc gccgatcacc
      121 accaggagga cgatgccgag tgcgagccag acgaccccgg cgatcagcaa cgtccgccga
      181 cgtcgcgagc cggcggccgg gttgggagca ccgtcggtgg aggtccgggt cacgtcggtc
      241 acggtcggaa ggcctcctcg atgccggctg ccccgtcctc ccaggacggc gggcgatacg
      301 tcgccagggc atcgcggcgt tcgcggtggt actccacgtc ggtgaggtgg cgctcgatga
      361 gttcgacgag ctcgtcgaca tcggtcgggt cgaagtactc agcccattcg ccgccggcct
      421 cgggcagggc accgccggtc gacgacaggg tcggtacacc gttgcccagc gcctcgatga
      481 ccggcgtgcc gaatccctcg tagaacgccg gctgcaccgc gaggaacgcg tggcggtaca
      541 ggccgtcgag caaccggtcg tcgaccttgt cgagccagcg gaaccttccg ccttggtcga
      601 cgtgggaccg catcagctcc tgcaggtcgt cggtcatcca tcccgccttg ccgaccatga
      661 ccaggccgag gtcggggtgg cgttccttga gacggtcgaa tgcgtcgatg agcaaccggt
      721 ggttcttgcg cggctccacc gtcgcaacgc tgagcaggta ccggccggtc ggtgcctcgg
      781 cggggagcgg caggtggtcg tcggcgcgct ggaagttcgc acccatcgtg atgacggagg
      841 tgtcgaggtc ggcggcgatg ccgagccgct cggcgagggc ctcgacgtcg cgccggctgc
      901 actcggagat gcagacgaac ttctccgagt gacgcaggtg cgcctcgatg aacgtcgaga
      961 acagtcggat ctgtccggcg tcgaaccact ggggaaactg ggtcggcatg acgtcggcga
     1021 ccagggtcga cgtgtgcaca ccgcggtcgc ggagccacgg cagcaccacg tcgcggcgtg
     1081 gcacgttgtg ccacgccgcc tcgaggtcga agaaccacga gtcggcgggc cactcgtcga
     1141 tgcggagctc cgcgtgcccg ggcgggtggt tgcggatgcg gcggcgacgc gccagctcct
     1201 cgcgcgccgc atgggcgatt cgggtgcgga tcagcgcccg gccgcggagc ttgagggtct
     1261 cggggagcgg atcggtgagc tgggcgagtc gtgaacgctg cggtgcgtcg cgtggtgtga
     1321 acacggcgag cgctgcacgt tccgcgaccg tgagccggcg gaaggtggcg cactcggcgc
     1381 accaccggac cggcacgaac gtgagcggac cttcgaggcg cccgaggcgc gcgagcagct
     1441 cccgggtgtg gcgttggaat ccggtgacgt agtcgacggc cagggtgttg gacacttcga
     1501 cccacaccgt gcgggtcggg gccggctggc gcgcgtcgga ggccggggat ggggaagctg
     1561 gggggaaggt catgagtcga tgtgctcggc ggtcagagct gcgaagcgtc gcccgatcgc
     1621 ccgccagtca caatgctcct cgacgtagtg ccgacccgca agcgagcgat ccgtgttgtc
     1681 ctggcgcacg acgtcgcgga tcccggccgc gagttcggcg aggggcacgg tcgccacgcc
     1741 ggcggcgtcg gcatcgatcc cacgtgcgcc gacgatggtg gacacgacgg ggagcccggc
     1801 ggccaggtac gtgagcagtt tcaggttcga accgctcccg gcgctcatgg ggttcagcgc
     1861 caggtcgcac gcagagagga gcaggtcgag caggtcgtcg gagaccggcc ccaggacgcg
     1921 gacgttgggt ggaaggtgcc ggacgttcag cagcgacgag tggcggccgg cgaggaggaa
     1981 gtcgatgtcg ggaacctcgc gggcgacctc gatgatgacc cgaccggcgt cgatgttcgg
     2041 gccgtgtccc gagcccacga acagcgcggc cgggcgtcga gggtcgagct cgacgtcctg
     2101 cgcgagggcc aggcggcgtc gtcgccggtc gccgccggcg acgaactcga tctcggtcgt
     2161 gtcgacgccg ttcggcacga ccgtcgtcgg agcagcgatc cggtagcgag cacccagggt
     2221 gtcgaggtcg agatcggtcg tcgccgtcac gagtgcggcg tgctcgaccg ctgttcgctc
     2281 gatgcggtcg acgtggccga ggagccatcg ccctgcggcg ttgtcgggaa tgatgctgcc
     2341 cttcaacgcg agttcgtcgt tgtgctcgtc gcagatgacg gggatgtcgc cggcgaacgc
     2401 tcgcacggcg tcgaccaggt acggctgcac gagcacggcg gcagaggccc cgtcgagctc
     2461 ctcgccgagg gcctcgacga actcatccaa ccccgaccac atgagaccgg cggcgatgtc
     2521 ggtcagcgac acgttgccgg tgacgagacg gaggtcggtc tcccgcgctg cgtgcgcggg
     2581 tgggagcgcg acgcagatct cgacgagccc tggagcgagc tcgtggcgcc cggcctggga
     2641 cggatcggtc gtgaggctga cgaccgagac gtcggtgagc ccgtcgtgtc cgagcgcctc
     2701 ggcgaggtgc caaccccgga gttggccgcc gctctgtcgc gggtggagcg ggtaggtgct
     2761 cacttcgacg aggcgtcgtc gcggtcgccg tgacgcacca cggtcgtgcg gcggcgccgg
     2821 tcgtgccgac gccgccgact caaccacgtg gtccatgaac gcgggaacgg tgcggtgggt
     2881 gacgcgggcg ctggttcggg cgaccgttcg ccgcaaggcg cgaacgcccg aggggatggg
     2941 cgattcggcg gagcggatcc acgccaggag cgcgtcaggc acccgatccg tccccgatgc
     3001 gctcggccca ttcgaccagc cgtcggccgg cctcgacgac gtcggcggtc gaaccggcga
     3061 aggaccaccg gatccaccgg tgaccgtcga cgggatcgaa gtcgaccccc ggtgccgtgg
     3121 cgacgcccag ctcgtggagc cactggtcga ccagctcgcg gctgtcgatg ccccaccgtg
     3181 cgacgttgcc gtacagatag aacgccccgt cgcagggcgc gagctcggtg acccccatgc
     3241 gctccagcgc gtcgaggagg gccgcacggt tcgcggcgta ccgctcgaca tggccgtcga
     3301 gttcggcgag gtcgtcgaac gcggcgagtg cggcgtgctg ggccagtgtg gggggcgaga
     3361 ggtagaggtt ctggctcagt cggtcgacgg gctcgacgag ttcgggcggc acgatcatcc
     3421 atccgagtcg ccaaccggtc atcgagaagt acttcgagaa gctgccgatc acgacctcgc
     3481 cggccgc
//
LOCUS       DAOSOA010000341         3476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_27154_length_3476_cov_4.758258, whole genome shotgun sequence.
ACCESSION   DAOSOA010000341 DAOSOA010000000
VERSION     DAOSOA010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27154_length_3476_cov_4.758258"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1067)
                     /locus_tag="PKY13_15315"
     CDS             complement(69..1067)
                     /locus_tag="PKY13_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOT81363.1"
                     /translation="MRAVVLDTYGGTDVLGLRDIPSPVLGPDEVLVDVVATALNRADL
                     LQRQGLYPGPSVLDANGNAYEVPGMEFSGTVAAVGARVTGAKVGDPVMGIVGGGSYAE
                     QLVTHERLLMPVPASVALADAAAIPEVFVTAFDALVAQGGLTTGRTALVHAGASGVGT
                     AAIQIAKAIGARIVVTTSTGKVERCRELGADVVVDYTTDDFVAASKEATGGRGVDVVL
                     DVIGGEYLARNLGSVGTGGRIIQVGVMGGGPAEVNLGVVLMKKAQIIGTTLRSRPIEE
                     KIAICRRFVTEVLPWFDLGALRPVIDRRYPLADIAAAHDYLAENTSVGKVLIDVMR"
     gene            1081..2193
                     /locus_tag="PKY13_15320"
     CDS             1081..2193
                     /locus_tag="PKY13_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT81364.1"
                     /translation="MDFSFSPELQALSDEACRVARETTSTLDVTEDSWLRGVDADFPQ
                     ILASHGWLGMCLPTEWGGGGRPAIERFAVVEALISEGAPIATAWFADRQIGPTLLQFG
                     TDEQRHRFLPDIIAGTSKWCVGMSEPDAGSDVASLRTRADRDGDHWVLNGQKVWTSGA
                     AESDWCYVIARTDPDAAPHAGLSEFIVDLHSPGVEIRTIHDMSDDRHFCEVHFSDVRV
                     PADLQVGTLNGAFKQIMRQMEHERGGIDRLVSNRRLYLDAVESARTTGRLASDPLLRQ
                     EYARLEGGYRIGRSLVLREVLGQAPAGYSAVTKTWCTEFEQRVAEFCVSIAGPDAMIW
                     GRTARNICYAPGYTIMGGTTQILRNIIGERILGLPR"
     gene            2228..>3476
                     /locus_tag="PKY13_15325"
     CDS             2228..>3476
                     /locus_tag="PKY13_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT81365.1"
                     /translation="MPVHFRTCPLCEATCGLEITTDDDGAITRIRGDRDDVFSKGFIC
                     PKGSTLKQLHADPDRVRTPLVKRDGEFVEATWDEAFAAVADGLGRIWADGDRDAVALY
                     LGNPNAHTLAGTLFGGQLVKAFGSKNLYSASTVDQMPKHVSSGYLFGNAGAIPVPDLD
                     RTDYLLMLGANPFESNGSLCTAPDFPGRMAAIRERGGRIVVVDPRRTKSADAADEHIA
                     IRPGSDALWLAALVNEIRIAGLVDVGAAADDIDGVDRVLAALEPFTAEAVAAHTRVPA
                     ETTRRIAREFAEAPSAVCYGRIGTHTTEFGTLASWLADVLSTVTGNLDQPGGAMFPRP
                     AHGRPAKPRAAGEPRPGGRGFSTGRWTSRVGGHPEVRGEMPVAALAEEIETDGPGKVR
                     ALITSSGNPVRSCPDSERLDAALA"
BASE COUNT          552 a         1281 c         1139 g          504 t
ORIGIN      
        1 gagcaggacc aacacggctg accagcgacg ccgaacatga ttcatggagc gaccctagag
       61 cggaagggtc acctcatgac gtcgatgagc accttgccca cgctcgtgtt ctcggccagg
      121 tagtcgtgcg ccgcggcgat gtcggcgagc ggatagcgac ggtcgatgac ggggcggaga
      181 gctcccaggt cgaaccacgg cagcacctcg gtcacgaagc gccggcagat ggcgatcttc
      241 tcctcgatcg ggcgcgaacg cagggtcgtc ccgatgatct gcgccttctt catgaggacc
      301 accccgaggt tgacctcggc cgggcccccg cccatcacgc cgacctggat gatgcgcccg
      361 cctgtgccga cgctgcccag gttcctggcg aggtactcgc cgccgatgac gtccaacacg
      421 acgtcgacac cgcgaccacc ggtcgcctcc ttggatgccg ccacgaagtc gtccgtcgtg
      481 tagtcgacga cgacgtcggc cccgagctcg cggcaacgct cgaccttgcc ggttgacgtc
      541 gtcacgacaa tccgggcgcc gatcgccttg gcgatctgga tcgccgccgt gccgacaccc
      601 gacgcgcccg cgtgcaccag ggcagtgcgg cccgtggtca gaccgccctg cgccacgagg
      661 gcgtcgaacg cggtgacgaa gacctcgggg atggctgcgg cgtcggcgag cgcgacgctg
      721 gcgggcaccg gcatcagcag acgctcatgc gtgaccagtt gttcggcgta ggacccgcca
      781 ccgacgatac ccatgacggg gtcgccgacc ttcgcacccg tcacgcgcgc accgacggcg
      841 gccacggtgc ccgagaactc catccccggc acctcgtagg cgttgccgtt ggcatcgagc
      901 accgacgggc cagggtacag gccctggcgc tgcagcaggt cggcgcgatt gagcgccgtc
      961 gcgaccacgt cgacgagcac ctcgtccgga ccgagaacag ggctgggaat gtcgcggaga
     1021 ccgaggacgt cggttccgcc ataggtgtcg aggaccacgg cgcgcatagc ctcaccgtat
     1081 atggacttct cgttctcccc ggagctgcag gcactgtccg acgaggcctg ccgggtcgcg
     1141 cgtgagacca cgtcgaccct cgacgtcacc gaggactcgt ggctccgcgg tgtcgacgcc
     1201 gacttccccc agattctcgc ctcgcacggc tggctcggca tgtgcctccc caccgagtgg
     1261 ggcggcggag gacgaccggc gatcgagcgg ttcgccgtcg tcgaggcgct catctccgag
     1321 ggcgccccga tcgccaccgc ctggttcgct gaccgccaga tcggcccgac gctcctgcag
     1381 ttcggcaccg acgagcagcg acaccggttc cttcccgaca tcatcgccgg cacctcgaag
     1441 tggtgcgtcg gcatgagcga gcccgacgcc gggagcgacg tcgcgtcgct ccgcacccgg
     1501 gccgaccgtg acggcgacca ctgggtcctc aacggccaga aggtctggac gtcgggagcc
     1561 gccgagtccg actggtgcta cgtcatcgcc cgcaccgatc ccgatgcggc gccccacgcc
     1621 ggcctctcgg agttcatcgt cgacctccac agccccgggg tcgagatccg caccatccac
     1681 gacatgagcg acgatcgcca cttctgcgag gtgcacttca gcgacgttcg tgtgcccgcc
     1741 gacctgcagg tgggcacact caacggcgcg ttcaagcaga tcatgcggca gatggagcat
     1801 gagcgcggcg gcatcgaccg gctcgtctcg aaccgccggc tgtacctcga cgccgtcgag
     1861 tcggcacgta ccaccggccg cctcgcgtcc gacccgttgt tgcgtcagga gtacgcccga
     1921 ctcgaaggcg ggtaccggat cggccggtcg ctcgtgctcc gcgaggtcct cggccaggcg
     1981 ccggccggat actccgccgt caccaagacg tggtgcaccg agttcgagca gcgcgtcgcc
     2041 gaattctgtg tctcgatcgc cgggccggat gcgatgatct ggggacggac cgcgcgcaac
     2101 atctgttacg cgcccggata caccatcatg ggtgggacca cgcagatcct gcgcaacatc
     2161 atcggcgaac ggattctcgg cctgccccgc tgacggggcc cggccgtcac accgtccggg
     2221 gggacacatg ccagttcatt tcaggacctg cccgctctgc gaggccacct gtggtctcga
     2281 gatcacgacc gacgacgacg gtgccatcac ccgaatccgt ggtgaccgag acgacgtgtt
     2341 ctcgaaaggg ttcatctgcc cgaagggatc cacgctcaag cagctccacg ccgacccgga
     2401 ccgcgtccgc actcccctcg tcaaacgcga cggtgagttc gtcgaggcca cgtgggacga
     2461 ggcgttcgcc gccgtcgccg acggcctcgg tcgcatctgg gccgacggcg accgtgacgc
     2521 ggttgcgctc tacctcggca accccaacgc gcacacgctc gccggcacgc tcttcggtgg
     2581 gcagctggtc aaggcgttcg ggtcgaagaa cctctactcg gcgtcgacgg tcgaccagat
     2641 gcccaagcac gtgtcgtcgg gttacctctt cggcaacgcc ggggcgattc ccgtgcccga
     2701 cctcgaccga accgactacc tcctcatgct gggcgcgaac cccttcgagt ccaacggcag
     2761 cctctgcacc gcccccgact tccccgggcg catggccgcc atccgcgaac gcggcggccg
     2821 catcgtcgtg gtcgacccgc gccgcaccaa gagcgccgac gcggccgacg agcacatcgc
     2881 catccgcccc ggctccgacg cgttgtggct ggcggcgctc gtcaacgaga tccggatcgc
     2941 cggactcgtc gacgtgggcg cagcggcgga cgacatcgac ggcgtcgacc gagtccttgc
     3001 ggcgctcgag ccgttcactg ccgaggcggt ggccgcccac acacgggtac ccgccgagac
     3061 gacccgccga atcgctcggg agttcgccga ggccccgtcg gccgtctgtt acggacggat
     3121 cggcacccac accaccgagt tcggaacgct ggcatcctgg ctcgccgacg tgctcagcac
     3181 cgtgaccggc aacctcgacc aacctggcgg cgcaatgttc ccccgtccgg cacacggtcg
     3241 accggcgaag ccgcgggccg ccggcgagcc acggccgggc ggtcgcgggt tctccaccgg
     3301 ccgctggacg tcgcgagtcg gtgggcaccc cgaggtccgc ggcgagatgc ccgtcgccgc
     3361 cctcgccgag gagatcgaga ccgacgggcc gggcaaggtg cgggcactga tcacgtcgag
     3421 tggcaacccg gttcgctcgt gccccgactc cgagcgcctc gacgcagcgc tcgcgt
//
LOCUS       DAOSOA010000342         3470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_27222_length_3470_cov_7.390922, whole genome shotgun sequence.
ACCESSION   DAOSOA010000342 DAOSOA010000000
VERSION     DAOSOA010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27222_length_3470_cov_7.390922"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PKY13_15330"
     CDS             <1..629
                     /locus_tag="PKY13_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81366.1"
                     /translation="KVLRLPSGDSDEMIIEYADAGERRRGAYFEPEGDFRVGQSIPIR
                     MLPGEGKYLIDGEIFMDESTSDAVGFGFLSALVAGPIAVWIGPRSRSLRRGLRSGPFR
                     RVKVIRYRVSSSGNVGVVEIDDGTKGGPRILRLRANYLGSWKGPDPWPGVRLTGELLV
                     AGPEWSERAAVAGPSPLQVIRTRVAPRRQRWQHWLDRKPETPVGPTDS"
     gene            639..1682
                     /locus_tag="PKY13_15335"
     CDS             639..1682
                     /locus_tag="PKY13_15335"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOT81367.1"
                     /translation="MATHEREITEPVDLCTANGRRLHPDAIGWSRHPILTANLRGSWG
                     RNKRWDYWAILAGDLAIAVTYADVDYLGMASIWWCDLETGRTGGRDNNIPGAFGIDLP
                     DLPGTAPLRWSNRRGGVALVDDEGGVTIDAAWRESDGTRASLTARIELPEGHESLNVV
                     IPWSETRFQFTSKHQARPAHGVLTIGDEERTFGSTGDAWGVLDVGRGRWPYRNIWNWG
                     GGAGHATEIERGSDHVVGLQFGGKWTQGTGHTENGIIVDGRLTKVGDELEWTYDWDAP
                     LRPWTLRAPDGSIDVTLHPRYDRHSRVEAVVLGMEVHQVFGHWSGTVRDDDGVTYRFD
                     GMQGFAEEARNRW"
     gene            1740..2225
                     /locus_tag="PKY13_15340"
     CDS             1740..2225
                     /locus_tag="PKY13_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HOT81368.1"
                     /translation="MNEPTDDRLRELGTLAFRIAYRLLGSRQDAEDVAQEAVVRTAIR
                     WRRVHGHADAFVGRVATNLAIGILRKRREVPETTTSSDGGVPNMADRLDLVRALRTLS
                     TRQREVVVLRFIGDFSERDIAATLGCSPGSVKKHCARGLAALRVALPHDTPIEGVLDA
                     H"
     gene            2215..3330
                     /locus_tag="PKY13_15345"
     CDS             2215..3330
                     /locus_tag="PKY13_15345"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT81369.1"
                     /translation="MPTDAALPDETPSFVPDDAFVAAVRHRVRRHRRRTRQRLGAGVA
                     LVAVIAGSGLWYTNDRLSSVQRIDLGTDPTAPDPVADGTPFNLLVVGLDECETADDDM
                     CANRLGLVPRDAPASADTIAVVHVDPAADTLDMLAVPRDLLADTNTKWSWLSVPQIRS
                     HLRDDLGIEVDHAVTMTMTGFRDLADDLTLRLSLGTAVRDRSSGLDLAAGCQRVDGAQ
                     LLALVRARHLEFRDGDGWRADQTGDIGRVARQQQVLRALFERLDDVDLGDARTLDRAL
                     SSIAVDDSLSNADVVRLARLARGSTLRSVAALPTHLGGDASMAFVSRRDAATVNPVIA
                     PFGGHVEDTAASPTSTAPPGMQSILDERESWTDISSC"
BASE COUNT          548 a         1293 c         1136 g          493 t
ORIGIN      
        1 cgaaagtgct ccggctcccg agcggcgact ccgacgagat gatcatcgag tacgcagacg
       61 ccggcgaacg gcgccggggc gcctacttcg aacccgaggg cgacttccgc gtcggtcaat
      121 cgattccgat ccgcatgctc ccgggcgagg gcaagtacct gatcgacgga gagatcttca
      181 tggacgagtc caccagcgac gccgtcgggt tcgggttcct gagcgcgctc gttgccggcc
      241 cgatcgccgt ctggatcggc ccgcgatcgc gctccctccg acgcggcctg aggtccggac
      301 cgttccggcg cgtgaaggtg atccgctacc gcgtctcgag cagcggcaac gtcggcgtcg
      361 tcgaaatcga cgacggcacc aaaggtggtc cccggatcct gcggctccgc gccaactacc
      421 tcggctcgtg gaaggggccc gatccgtggc cgggtgtccg gctcacgggc gaactcctgg
      481 tcgccggacc ggagtggtcc gaacgggccg cagtggcggg tccctcccca ctgcaggtca
      541 tccgcacccg cgtcgccccc cgacgccaac gctggcaaca ctggctcgac cgcaagccgg
      601 agacgcccgt cggccccacc gactcgtagt ctccgggcat ggcaacccac gaacgcgaga
      661 tcaccgaacc ggtcgacctc tgcacggcca acggccgacg tctgcacccc gacgcgatcg
      721 gctggtcgcg gcacccgatc ctgacggcca acctgcgggg ctcatggggt cgcaacaagc
      781 gatgggacta ctgggcgatc ctcgccggcg acctcgccat cgccgtcacc tacgccgacg
      841 tcgactacct cggcatggcc tcgatctggt ggtgcgatct cgagacggga cgcaccggag
      901 gtcgcgacaa caacatcccc ggagcgttcg gcatcgacct ccccgacctg cccggtacgg
      961 caccgctgcg gtggtccaac cggcgcgggg gcgtcgccct cgtcgacgac gaaggcggcg
     1021 tgaccatcga cgccgcctgg cgtgaatccg acggcacccg agcctcgctc accgcccgga
     1081 tcgaactgcc ggagggccac gaatcactca acgtcgtcat cccctggagc gagacccgct
     1141 tccagttcac gagcaagcac caggcccgtc cggcccacgg tgtgctcacg atcggcgacg
     1201 aggagcggac gttcggatcg acgggcgacg cgtggggtgt gctcgacgtc ggccgagggc
     1261 gttggccgta tcgcaacatc tggaactggg gcggcggcgc cggacacgcg accgagatcg
     1321 agcgcggatc cgaccacgtc gtcggcctgc agttcggcgg gaagtggacc cagggcacgg
     1381 ggcacaccga gaacggcatc atcgtcgacg gccgtctcac caaggtcggc gacgaactcg
     1441 agtggaccta cgactgggac gcgccacttc gtccgtggac cctccgtgcg cccgacggtt
     1501 cgatcgacgt cacgctccac ccccgctacg accgtcacag ccgcgtcgag gcggtcgtgt
     1561 tggggatgga ggtccaccag gtgttcgggc actggtcggg caccgtccgt gacgacgacg
     1621 gcgtgacgta ccggttcgac gggatgcagg ggttcgccga ggaggcccgc aaccgctggt
     1681 gagcccgatt cgcgccggcc ggtgtcaacc gaacgagtcg ccggcgcgtt gacctggtga
     1741 tgaacgagcc caccgacgac cgcctccgcg aactcggaac cctcgcgttc cggatcgcgt
     1801 atcgactcct cgggtcccgc caggacgccg aggacgtcgc ccaggaagcg gtcgttcgca
     1861 cggcgatccg ctggcgacga gtacacgggc acgccgacgc cttcgtcggt cgggtcgcga
     1921 ccaacctcgc catcggcatc ctgcgcaaac gccgtgaggt tccggagacg acgacgtcat
     1981 cggacggcgg cgtccccaac atggccgacc gactcgacct ggtgcgggcg ctccgcacgt
     2041 tgtcgacccg ccaacgcgaa gtggtggtgc tccggttcat cggcgacttc tccgaacggg
     2101 acatcgccgc gaccctcggt tgctcgcccg gttcggtcaa gaagcactgc gcccgcgggc
     2161 tcgccgccct ccgcgtcgcg ctcccccacg acaccccgat cgaaggagtc ctcgatgccc
     2221 actgacgctg ccctccccga cgagaccccc tcgttcgtgc ccgacgacgc gttcgtggcc
     2281 gcggtgcggc accgggtgcg tcgccaccgc cgtcgtaccc gccagcgact cggcgccggt
     2341 gttgcactcg tcgccgtcat cgccggcagc gggctctggt acaccaacga ccggctgtcg
     2401 tcggtgcaac gcatcgacct cggcaccgac ccgaccgcgc ccgatcccgt cgccgacggc
     2461 accccgttca atctcctcgt ggtcggcctc gacgagtgcg agacggccga cgacgacatg
     2521 tgtgccaacc ggctcgggct cgtaccccgc gacgctccgg cgtcggccga caccattgcc
     2581 gtcgtgcacg tcgatcccgc agccgacacg ctcgacatgc tcgccgtccc ccgtgatctc
     2641 ctcgccgaca cgaacacgaa gtggtcgtgg ctgtcggtcc cacagatccg gtcgcatctg
     2701 cgggacgatc tgggcatcga ggtcgaccac gcggtcacga tgaccatgac cgggttccgc
     2761 gatctcgccg acgacctgac gctccggctg tcgctcggta ccgcggtgcg agatcggtcg
     2821 agcggcctcg atctcgccgc gggatgccaa cgcgtcgacg gcgcccagct gctcgccctc
     2881 gtgcgagccc gacacctcga gttccgcgac ggcgacgggt ggcgcgccga ccaaaccggc
     2941 gacatcggcc gggtcgcccg ccaacagcag gtgctgcgag cgctgttcga acggctcgac
     3001 gacgtcgacc tcggcgatgc gcgcacgctc gaccgagcgc tctcgtcgat cgccgtcgac
     3061 gactccctgt ccaacgccga tgtggtgcgt ctcgcccggc tcgcccgggg ctcgacgctc
     3121 cgctcggtgg cggcgctccc cacccacctc ggcggcgatg cctcgatggc gttcgtcagc
     3181 cgacgcgacg cagccacggt caatccggtg atcgcaccgt tcggcgggca cgtcgaggac
     3241 acggcagcgt caccgacctc gacggcaccg cccgggatgc agtcgatcct cgacgaaagg
     3301 gagagctgga ccgacatctc gtcctgctga ccgggcccgg gccacaatgg ccggcatgaa
     3361 gttcacggcc accatccatc aggaaggcga ggcggcaacc ggaatcccgg tccctcccga
     3421 gacccgccag cgccgagtcg acaaggcgcc cgagaccttg cgcaacggtt
//
LOCUS       DAOSOA010000343         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_27276_length_3464_cov_5.226753, whole genome shotgun sequence.
ACCESSION   DAOSOA010000343 DAOSOA010000000
VERSION     DAOSOA010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27276_length_3464_cov_5.226753"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1193)
                     /locus_tag="PKY13_15350"
     CDS             complement(135..1193)
                     /locus_tag="PKY13_15350"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOT81370.1"
                     /translation="MSALSTPLCDLLGVRYPIVQTGMGWVSGARLTAATCEAGAMGIL
                     ASATMTLPELRVAIGKVKARTSNPFGVNLLPNQADLLDRIDLMITEGVTVASFAGAPS
                     GDVVARLNDAGLVTMVTVGAKRHAEKMVKMGVKALIAQGGEGGGHTGATPTSLLLPAV
                     VDATEGSGVPVLGAGGYYDGRGLVAALAYGAQGIAMGTRFLLTQESHVPDHVKAIYVG
                     TPVTGTVVTTAIDGAPQRVIRTEMIDELEKAPSVVRFPKAALNALKFARLSGTPIKDL
                     VTSGLAMRKAQDLTWAQMALAANAPMMTKAGVVDGRVEAGILPTGQVVGSIEELPTVA
                     DVIAGIVDEAEATLERLS"
     gene            complement(1344..>1898)
                     /locus_tag="PKY13_15355"
     CDS             complement(1344..>1898)
                     /locus_tag="PKY13_15355"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOT81371.1"
                     /translation="AVVEAWNPYPQMFSVLWNGKRHVMMGATQVDKWGNQNIACIGDW
                     HQPRSQLLGFRGAPGNTINHTTSYWVPNHGTKVFVPAVDVVCGVGYDRARELGPVASR
                     FHEIRRVVSNLGVFDFDTADNSMQLRSVHPGVTVDEVVAATGFELVIPSEVPESRLPT
                     DEELRLIREVIDPKGLREREVPNP"
     assembly_gap    1899..1998
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1999..2834)
                     /locus_tag="PKY13_15360"
     CDS             complement(<1999..2834)
                     /locus_tag="PKY13_15360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOT81372.1"
                     /translation="MAIPDKRTTEAEVVSGLRDGMTIGIGGWGSRRKPMSLVRELLRS
                     DLKDLTVVSYGGPDVGLLCRAGKVKRAVYGFVSLDSIPLEPHFRNARQNGTVEATELD
                     EGMLLLGLQAAAWRVPFLPCRAGLGTDLLRVMPQFELVRSPYPGPDGGEGEELVAVPA
                     IELDVAFIHMNRADQGGNGQFLNQDLYMDDLMAMAAKECFMSTEEIIPTEDFPKYGSF
                     HTQRISRLMTTGVIERKGAAHFTECPPDYARDEAFQKEYAATAKSDEAWDAFRAKYLD
                     VS"
     gene            3000..>3464
                     /locus_tag="PKY13_15365"
     CDS             3000..>3464
                     /locus_tag="PKY13_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81373.1"
                     /translation="MHVRRLTVAALSLLLPFTAAACGGDDDKADSDTTTTTASDSATT
                     VAPADDPADTTPDAPTTTLSEADFNAAIDAFNTSLDEAGDDFCKITAATNTLTVSSAA
                     TPEQAKALYQAFADVLNKVADHLPVDSGADSAPLKSAAEKILSDAEADGFDPA"
BASE COUNT          524 a         1189 c         1123 g          528 t
ORIGIN      
        1 acggggcagg cgggtcgatc gagagcaccg aggcggcgcg ggcacagccc gccgcctcgc
       61 cgcgcccgtg aacgcggtcg cactggtcgg gatggtcggg atggtcggga tggcgacggt
      121 tcaggcgagg cgggtcagct caagcgctcg agggtcgcct cggcttcgtc gacgatgccg
      181 gcgatcacgt cggcgaccgt cggcagttcc tcgatggaac cgaccacctg cccggtcggc
      241 aggatgcccg cctcgacccg accgtcgacg acgccggcct tggtcatcat cggcgcgttc
      301 gcagcgaggg ccatctgcgc ccacgtgagg tcctgcgcct tgcgcatggc gaggccactc
      361 gtgacgagat ccttgatcgg tgtgcccgac aaccgagcga acttgagcgc gttgagtgcg
      421 gccttcggaa aacgcacgac cgacggcgcc ttctccagct cgtcgatcat ctcggtgcgg
      481 atcacgcgct gcggcgcgcc gtcgatcgcc gtggtgacca cggtgccggt cacgggggtg
      541 ccgacgtaga tcgccttcac gtggtccggg acatgggact cctgcgtcag caggaaccgg
      601 gttcccatgg cgatgccctg cgcgccgtag gcgagcgccg cgacgagccc acgcccgtcg
      661 tagtagccac cggcgccgag caccggcaca cccgagccct cggtcgcgtc gacgacggca
      721 gggagcagga gcgacgtggg ggtcgctcct gtgtggccgc cgccctcgcc gccctgggcg
      781 atgagggcct tgacgcccat cttcaccatc ttctcggcgt gacgcttcgc gccgacggtc
      841 accatggtga ccaggcccgc atcgttgagc cgcgcgacga cgtcacctga cggtgccccg
      901 gcgaacgacg cgaccgtgac cccttcggtg atcatcaggt cgatgcggtc gagcaggtcg
      961 gcctggttcg gcaggaggtt cacgccgaac gggttggagg tccgcgcctt caccttgccg
     1021 atggcgacac ggagctccgg caacgtcatc gtcgccgacg ccaggatgcc catggcgccg
     1081 gcctcgcacg tcgccgcagt gaggcgcgca ccggagaccc aacccatgcc ggtctgcacg
     1141 atcgggtacc ggacgccgag cagatcgcac aacggcgtgc tcagcgcact cacggattcg
     1201 gcctcggcgt cgggcgctcg ggacgcttcg cgccctcggc cctcctggca ctgtcacatg
     1261 ggtgcctcac gggttcggcc tcggcgtcgg gcgctcggga cgcttcgcgc cctcggccct
     1321 cctggcactg tcacatgggc gcctcacggg ttcggcacct cgcgctcacg gagacccttg
     1381 gggtcgatga cctcacggat caggcggagc tcctcgtcgg tcggcaggcg ggactcgggg
     1441 acctcgctcg ggatcacgag ctcgaagccg gtcgcagcga ccacctcgtc gaccgtcacg
     1501 ccggggtgca ccgagcgcag ctgcatcgag ttgtcggcgg tgtcgaagtc gaagacgccg
     1561 aggttgctga cgacgcgtcg gatctcgtgg aatcgcgacg ccacgggacc gagctcgcga
     1621 gcgcggtcgt agcccactcc gcagacgacg tcgaccgccg gcacgaacac cttggtgccg
     1681 tgattgggca cccagtagct cgtcgtgtgg ttgatcgtgt tgcccggcgc gccgcggaac
     1741 cccaggagct gggaccgggg ctggtgccag tcgccgatgc acgcgatgtt ctggttgccc
     1801 cacttgtcga cctgcgtcgc gcccatcatc acgtggcgct tgccgttcca caggacgctg
     1861 aacatctgcg ggtacgggtt ccaggcctcg acgacggcnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnntc ggacacatcc aggtacttcg cccggaacgc gtcccacgcc
     2041 tcgtcggact tcgccgtcgc cgcgtactcc ttctggaacg cctcgtcgcg ggcgtagtcg
     2101 ggcgggcact ccgtgaagtg ggcggcaccc ttgcgctcga tcacaccggt ggtcatcagc
     2161 cggctgatgc gctgggtatg gaacgagccg tacttcggga agtcctcggt cgggatgatc
     2221 tcctcggtgc tcatgaagca ctccttggct gccatggcca tgaggtcgtc catgtagagg
     2281 tcctggttca ggaactggcc gttgccaccc tgatcggcgc ggttcatgtg gatgaaggcc
     2341 acatcgagtt cgatcgccgg gacggccacc agctcttcgc cctcgccacc gtcgggcccg
     2401 ggatacggcg agcgcaccag ctcgaactgc ggcatcacgc ggagcaggtc ggtaccgagg
     2461 ccggcacggc agggcaggaa ggggacccgc cacgccgctg cctgcagacc gaggagcagc
     2521 atgccctcgt cgagctcggt cgcctcgacc gttccgttct ggcgagcgtt gcggaagtgc
     2581 ggttcgagcg ggatcgagtc gagggacacg aagccgtaga cggcccgctt gaccttgccg
     2641 gcacgacaga gcagcccgac atcgggcccg ccgtagctga cgacggtcag gtccttcagg
     2701 tcgcttcgaa ggagctcacg cacgagcgac atcggcttac gtcgggagcc ccagcccccg
     2761 attccgatcg tcatgccgtc gcggagcccg ctcacgacct cggcctcggt cgttcgcttg
     2821 tcaggtatcg ccatcaggtc ctcatcggtc gcaggtggat cgggtccgca ccgccgctcg
     2881 ggcgaatctg acggaccgtc agatcgagtc caaaccgtag accggtcgct ggttgcgcgt
     2941 cgactgcgac gaagtccaga tagcgtgatc gtcagtaccc cgacccagag gatcttctcg
     3001 tgcacgtccg tcgactcacc gtcgctgccc tcagcctgct ccttccgttc accgccgccg
     3061 cctgcggtgg tgacgatgac aaggccgatt ccgacacgac gaccacgact gcgtccgact
     3121 cggccaccac cgtcgcaccc gccgacgatc cggcggacac cacccccgac gccccgacga
     3181 ccacgctgag cgaggccgac ttcaacgcag cgatcgacgc gttcaacacg agcctcgacg
     3241 aggccggcga cgacttctgc aagatcaccg cggcgaccaa cacgctgacg gtgtccagtg
     3301 cggcaacccc cgagcaggcc aaggccctct accaggcgtt cgccgacgtg ctgaacaagg
     3361 tcgccgacca cctccccgtc gactctggcg ccgacagcgc ccccctcaag agcgccgcgg
     3421 agaagatcct gagcgacgca gaggccgacg gctttgaccc ggcc
//
LOCUS       DAOSOA010000344         3455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_27380_length_3455_cov_4.493235, whole genome shotgun sequence.
ACCESSION   DAOSOA010000344 DAOSOA010000000
VERSION     DAOSOA010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27380_length_3455_cov_4.493235"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PKY13_15370"
     CDS             complement(<1..831)
                     /locus_tag="PKY13_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81374.1"
                     /translation="MTTVEIDGSLDGPLDGGRCAGPPERHLPPRLRHWRQRTSTPLLV
                     IGIGTLPLLLLELVRDDLGYRDRVFLDVVNVGVLALFLLDYLIELSLARGHRREFVRA
                     EWAQALIVLAQIAALAPMLAGAGAVRAVRGARALRGVAAILRALALGGAVAADGRRAL
                     RRHAAGFAMAVAGLTWISSAVGFTLVEDVGAKGEWTSFFDAVWWSPYPTVVMVHVDHH
                     TASKNEVHSPFAPTSSTNVKPTAELTHVRPATAMAKPAAWRRSARRPSAATAPPRGSA
                     R"
     gene            complement(842..2047)
                     /locus_tag="PKY13_15375"
     CDS             complement(842..2047)
                     /locus_tag="PKY13_15375"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOT81375.1"
                     /translation="MSDTPMDDSWWLASDGKWYPATSHPNYQPPPRPDPPSPPEPDGP
                     PGPTVIGTPGSGPAEVANRWQQGADGERATADALMKLPSTYRIIHGLKVGAGKGDIDH
                     LVVGPTGVWVIDSKNDAGEFTAGNGTLWKGKYPISQKVESVEAQARYARFALGVDANP
                     VLCFVQAKLPRPAQMVGRVRVVELDALAQHIMAGQTLMLPQHIEGTVGRVGAWISRPP
                     IMPTSVPPVRPEPAPRSRSRRSGPPQSVPPASERGGSGNRRRRSPLTALVSGLIAIVL
                     VVGACSVALVMAGKAAENLTERTRATTTTLVGAGYLKVDIDCPAPNGGHRMKPRSVAE
                     LGDEIRVSATINGAAQYLGQFRSYQPIEPIRGLAPNTTVGFDIQLVDDVGQGGPSYHL
                     DITAPATPC"
     gene            complement(2131..2583)
                     /locus_tag="PKY13_15380"
     CDS             complement(2131..2583)
                     /locus_tag="PKY13_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81376.1"
                     /translation="MLNVLVSTLETQGSVPGDFSYVPAGELVGRYSLVCDSERPDGTG
                     CGCGRAFGGFTTHRSTTTAIVVERDMTEADWRKALYDTLVATGWAGLMRDDALAGLVD
                     DLVEHDLHHIAEIPAGTVLGRRAYNEGRGDDANTVDTLLERTRLPRLG"
     gene            complement(2644..>3455)
                     /locus_tag="PKY13_15385"
     CDS             complement(2644..>3455)
                     /locus_tag="PKY13_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81377.1"
                     /translation="GVWVVIAANEPGVLVKQAGTLWNGDTSISAKVEQVETLARFARK
                     VLGCDANSVLCFTTATLPRRAQMVGRVRVLELGVLADHLSGGHPTLGPDRVAAVLARV
                     DEWLRQAPALSTTPPPPPRGASLRRRIGIGAGIAAAIVTGATLSASFLTDVSVARTES
                     APPPSATDPTTTIGAPEELLEVDISCPRLGLGYQLIPRTVSTGLDLLQVAATVDGQPQ
                     FIGSLAAGLPGPPIIGVPPSTTVGFDYVVVGTDESTGPPNHVDISTPPWPC"
BASE COUNT          530 a         1166 c         1236 g          523 t
ORIGIN      
        1 ccgcgcgctt cctctcggcg gggcggtagc tgccgacgga cgacgagcac tccgccgcca
       61 cgccgcaggc ttcgccatgg cggtcgccgg cctcacatgg gtcagctcgg cggtgggctt
      121 cacgttggtg gaggacgtcg gggcgaaggg cgagtggacc tcgttcttcg atgcggtgtg
      181 gtggtcgacg tgcaccatca ccacggtggg gtacggcgac caccacacgg cgtcgaagaa
      241 cgaggtccac tcgcccttcg ctccgacgtc ctccaccaac gtgaaaccca ccgccgagct
      301 gatccacgtg aggccggcga ccgccatggc gaatcctgcg gcgtggcggc gaagtgctcg
      361 tcgtccgtcg gcagccaccg cgcccccgag agcgagggcg cggagaatcg cggcgacccc
      421 gcggagcgcc cgcgccccgc gaacagcgcg taccgctccg gccccggcga gcatcggtgc
      481 caacgccgcg atctgggcga ggacgatcaa cgcttgcgcc cactccgcgc gaacgaactc
      541 gcgtcgatga ccccgggcga gtgagagctc gatcaggtag tcgaggagaa acagcgcgag
      601 cacaccgacg ttgacgacat cgaggaacac ccggtcgcga tatccgagat cgtctcggac
      661 cagctccagg agcagcagcg gaagcgtgcc gatcccgatg accaacagcg gggtcgaggt
      721 ccgttgtcgc cagtgacgca ggcgcggtgg caggtgacgt tctggcggac cggcgcagcg
      781 gccgccgtcg agtgggccgt cgagcgatcc gtcgatctcg acggtggtca cgctccgcgc
      841 ctcagcatgg tgtcgccggc gccgtgatgt cgaggtggta gctcggtcca ccctggccga
      901 catcgtcgac cagttgaatg tcgaacccga ccgtggtgtt cggagcgagg ccgcggatcg
      961 gttcgatcgg ttgatacgaa cggaactggc cgaggtactg cgccgccccg ttgatggtgg
     1021 cggagactcg gatctcatcg ccgagctcgg cgaccgaccg tggcttcatt cgatggcccc
     1081 cgttcggtgc ggggcagtcg atatcgacct tcagataccc ggcgccgacg agcgtggtcg
     1141 tcgtggcgcg ggtccgctcg gtcaggttct cggcggcctt gccggccatc accagcgcga
     1201 cactgcacgc gccgaccacg aggacgatgg cgatcagccc actcacgagt gccgtcaacg
     1261 gtgatcgtcg acggcgattc cccgagcctc cgcgttcgga cgcaggtgga accgactgtg
     1321 gcggcccgct cctgcggcta cgtgaccgcg gggcgggttc aggacgcacc ggcggcaccg
     1381 aggtcggcat gatcggcggg cgactgatcc acgcgccgac ccgcccgacc gttccctcga
     1441 tgtgttgggg caacatcaag gtctggcccg ccatgatgtg ctgcgcgagg gcgtcgagct
     1501 cgacgacccg cacccgtccc accatctgag ccggtcgcgg cagcttcgcc tgcacgaagc
     1561 acaacaccgg gttggcgtcg acgccgagag cgaagcgggc atagcgtgcc tgcgcctcga
     1621 cggactcgac cttctgggag atcgggtact tacccttcca gagcgtgccg ttgccggcgg
     1681 tgaactcccc ggcgtcgttc ttggagtcga tcacccagac cccggtcggt ccgacgacga
     1741 ggtggtcgat gtcgcccttg ccggctccca ccttgagccc gtggatgatg cgataggtcg
     1801 acggaagctt catcagggcg tcggccgttg cccgctcgcc gtcggcgcct tgctgccatc
     1861 ggttggcaac ctccgcaggg cccgatcccg gagttccgat gaccgtcggt ccgggtggcc
     1921 cgtcgggctc tggtggggag gggggatcgg ggcgaggtgg cggctggtag ttcggatggg
     1981 acgtggcggg gtaccacttg ccgtccgacg caagccacca cgagtcgtcc atcggtgtgt
     2041 cgctcatcgc cccgccccca tcgtgatggg gaagaacaat atccggcaga gagcgagcgg
     2101 caaggtggag tcggggcgcc cagacgcccg tcagccaagc cgcggcagcc gggtccgctc
     2161 gagcagcgtg tcgacggtgt tggcgtcgtc tccgcgcccc tcgttgtacg cccggcgccc
     2221 gagcacggtg ccggcgggga tctcggcgat gtggtgcagg tcgtgttcca cgagatcgtc
     2281 gacgaggccc gcgagggcgt cgtcgcgcat cagccccgcc cagccggtgg cgaccaaggt
     2341 gtcgtacagc gccttccgcc agtcggcctc ggtcatgtcg cgttcgacga cgatggccgt
     2401 ggtggtgctg cgatgggtcg tgaacccacc gaaggcgcga ccacaaccgc agcccgttcc
     2461 gtccgggcgt tcgctgtcgc acaccaggct gtaccgaccg acgagttcgc cggcgggtac
     2521 gtaggagaag tcgcccggaa ccgagccctg tgtttcgaga gtggagacaa gcacgttgag
     2581 catgtcggcg agcatctcat cgacgcctga cagtgaggtc ggctcgttca gcagggccct
     2641 cgttcagcag ggccagggcg gcgtggagat gtcgacgtgg ttcggcgggc cggttgattc
     2701 gtcggtgccg acgacgacgt agtcgaatcc gaccgtggtc gacggtggga ctccgatgat
     2761 cggtggtccg gggaggccgg cggcgagcga cccgatgaac tgcggctgtc cgtcgacggt
     2821 cgccgcgacc tgcaacaggt cgaggcccgt cgacacggtc cgcgggatga gctggtagcc
     2881 gagaccgagc cggggacagc tgatgtcgac ctccaacagc tcctccggcg ccccgatcgt
     2941 cgtcgtcgga tcggtggcac tcgggggagg tgccgactcg gtccgcgcca cggacacgtc
     3001 ggtgaggaac gacgcactca acgttgcccc ggtgacgatg gcggcggcga tgccggcgcc
     3061 gatcccgatc cgtcgtcgga gcgacgctcc ccgaggcggc ggcgggggcg tggtcgacag
     3121 ggccggcgcc tgccggagcc actcgtcgac ccgagcgagc accgccgcga cccggtccgg
     3181 cccgagcgtc gggtggccac ccgagaggtg atcagcgagg accccgagct cgaggacccg
     3241 gacccggccg accatctgcg cccggcgcgg cagggtcgcc gtcgtgaagc agaggaccga
     3301 gttggcgtcg caaccgagca ccttccgtgc gaaccgggcg agcgtctcga cctgctcgac
     3361 cttcgccgag atggaggtgt caccgttcca caacgtcccc gcctgcttga ccaggacgcc
     3421 cggctcgttc gctgcgatga cgacccacac cccgg
//
LOCUS       DAOSOA010000345         3451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_27424_length_3451_cov_8.626914, whole genome shotgun sequence.
ACCESSION   DAOSOA010000345 DAOSOA010000000
VERSION     DAOSOA010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27424_length_3451_cov_8.626914"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKY13_15390"
     CDS             complement(<1..743)
                     /locus_tag="PKY13_15390"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOT81378.1"
                     /translation="MIDVRIDDLREPIRDEAEQRIYEMAMTMEVDLSPAALVAAANRR
                     TGLDDFGDETLLDRLAAQVSAVDADTGLSGLGRFLVRRRLVGILAARLRLEDFVKRHP
                     EALEVELEPPVIVVGLPRSGTTHLVNLLATDTRFRSLPWWEVIEPIPVRGDGPGRDGL
                     DPRYLRCEAEYLAAKEVSPLTALMHDRLPSSIEEECELLDLDLCTYVLEWLARVPSWR
                     DHAEQLDQFAHYAFLRRELQVLSYLRGPNR"
     gene            complement(740..1324)
                     /locus_tag="PKY13_15395"
     CDS             complement(740..1324)
                     /locus_tag="PKY13_15395"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT81379.1"
                     /translation="MSVEVVMAKPGRPRDHRLDEAIVAATIDLLGERGYNGLSLAGVA
                     ERAGTTTPAIYRRWSSKADLVLSTVFRTHGDDVVADTGDLDADIRSMVRWSLEKFGSP
                     AGRAALAGLLGEPSRPGAGPSPELSLVWGRTGERLARAVERGEIRADIDTDGLILQLA
                     GPAMMATAVHGSEAVSEEWVERIATVILDGVRAR"
     assembly_gap    1457..1556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1562..2371)
                     /locus_tag="PKY13_15400"
     CDS             complement(1562..2371)
                     /locus_tag="PKY13_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT81380.1"
                     /translation="MNVALDGITVTIDGAEIVGDVSVEIRSGEMHGVVGPNGSGKSTL
                     LRCLYRALRPASGRAMIDGDDIWRQLTARQAAQRRAIVPQDSSIDFDFSVRETVSMGR
                     APHQRLLDRNSATDTEIIDDALDTVAMTTFGARLVSTLSGGERQRVLLARALAQQAPV
                     LVLDEPTNHLDIRAQLELLELVRSLPTTTIAALHDLDHAASLCDAVTVLAHGRVVASG
                     PPDEVLTPDVIGQVFGVRAHVGTHPLTGRPHITVATRADQPARDTDRTTEP"
     gene            complement(2368..>3451)
                     /locus_tag="PKY13_15405"
     CDS             complement(2368..>3451)
                     /locus_tag="PKY13_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT81381.1"
                     /translation="RRSPMPSLPRAETRTRSSPHLIGGGGYGALLVALVLGLVVALVA
                     GISIGTVTLPVKEVWSVVGHHLGVLSAAPTKVQDQIVWDFRTPRVLLAAVVGAALSVA
                     GACLQVLGRNALADPYVLGVSSGASFGAVLVLAYGTGVSGGLSVTGAAFVAAMLTLFV
                     VFVLAQRGGRVGPSRLVLAGVAVSYLATAGTSFVQLYTRPAELRGLMFWILGSFASAS
                     WDDLGVPTVVIAVTAAWLLVRARSLNVMSTGDDSASALGVDVNRFRIELLIISSLLTA
                     CAVAVAGGIGFVGLMVPHACRFLVGPDHRRLLPVALLSGASFMVAVDLLARVVDRPNE
                     LPVGIFTAAVGAPFFLVLMRRSGATR"
BASE COUNT          553 a         1123 c         1213 g          462 t
ORIGIN      
        1 cggttcgggc ctcgcaggta gctgagcacc tgcaactcgc gacgcaggaa cgcgtagtgg
       61 gcgaactggt cgagctgctc ggcatggtcc cgccaactcg gcacccgggc cagccactcg
      121 aggacgtagg tgcacaggtc gaggtcgagg agttcgcact cctcctcgat gctggacggc
      181 agtctgtcgt gcatcagcgc cgtgagcggt gacacttcct tggccgcgag gtactccgcc
      241 tcgcagcgca ggtagcgcgg gtcgagcccg tcgcggccgg gcccgtcacc ccggacgggg
      301 atcggttcga tcacctccca ccagggaagg gagcggaacc gggtgtcggt cgccagcaga
      361 ttcacgaggt gggtcgtgcc cgatcgcggg agaccgacga cgatcacggg cggttccagt
      421 tcgacctcga gcgcttcggg gtgccgtttc acgaagtcct cgaggcgaag ccgcgccgcg
      481 aggatcccga cgagtcggcg gcgcacgagg aaccggccca gccccgagag gccggtgtcg
      541 gcgtcgacgg ccgacacctg cgcggccagg cggtcgagca gggtctcgtc gccgaagtcg
      601 tcgaggcccg tgcgccgatt cgccgcggca acgagggcgg ccggcgacag atcgacctcc
      661 atcgtcatcg ccatctcgta gatgcgttgt tcggcctcgt cgcggatcgg ctcgcggagg
      721 tcgtcgatgc ggacgtcgat catcgggccc gcaccccgtc gaggatgacc gtcgcgatcc
      781 gctcgaccca ctcctcgctc acagcttcgg atccgtgcac cgcggtcgcc atcatcgccg
      841 ggcccgccag ctggaggatc aggccgtcgg tgtcaatgtc ggcgcgaatc tcgccacgct
      901 cgaccgcacg agccagccgt tcgccggtgc gcccccacac cagcgacaac tccggcgagg
      961 gccccgcgcc gggacgagac ggttcgccca gcaggccggc cagcgcggcg cgtccggctg
     1021 gcgacccgaa cttctcgagg ctccaccgca ccatcgaccg gatgtccgca tcgagatcgc
     1081 ccgtgtcggc gacgacatcg tcgccgtggg tgcggaacac cgtcgacagc acgagatcgg
     1141 ctttcgagga ccatcgccga tagatcgccg gggtcgtggt gccggctcgc tccgcgaccc
     1201 cggcgaggga cagcccgttg tagccacgct cgccgaggag gtcgatcgtg gcggcgacga
     1261 tggcctcgtc gagccggtgg tctcgcggcc tgccgggctt ggccatgacc acctcgacgc
     1321 tcatattacg gaaggtatcg tatcgaaata gaacggtcca gcgacccgag gcgtcagagc
     1381 gcgtggaccg tcacgttgcg tcgagcatcg cctggtacat cgcccggagc tggtcggtga
     1441 tcgctggtgg agtcctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcgg
     1561 gtcacggttc ggttgtcctg tcggtgtcgc gggcaggctg gtcggcacgg gtcgccaccg
     1621 tgatgtgggg ccggccggtg agcgggtggg ttccgacgtg ggctcggact ccgaagacct
     1681 ggccgatcac gtcaggcgtg agcacctcgt cgggcgggcc gcttgcgacc acgcgaccgt
     1741 gggcgagcac ggtgacggcg tcacacagcg acgcagcgtg atcgaggtcg tggagcgcgg
     1801 cgatcgtggt ggtcggcagc gagcggacga gttcgagcaa ctccagctgg gcacggatgt
     1861 cgaggtggtt ggtgggttcg tcgagcacga gcaccggcgc ctgttgggcg agcgcccgcg
     1921 cgagcaggac gcgttggcgt tcaccgccgg acagggtcga gacgaggcgc gctccgaagg
     1981 tggtcatggc aaccgtgtcg agggcgtcgt cgatgatctc ggtatcggtg gcgctgttgc
     2041 ggtcgagcag gcgttggtgc ggggcacggc ccatggacac ggtctctcgg acggagaagt
     2101 cgaagtcgat gctgctgtcc tgagggacga tcgcccgccg ctgcgcggcc tgtcgggcag
     2161 tcagctgtcg ccagatgtcg tcgccgtcga tcatcgctcg tccgctcgcc ggccgcagcg
     2221 cccggtacag gcaccgcaac agcgtgctct tccccgatcc gttggggccg acgacgccgt
     2281 gcatctcgcc cgagcggatc tcgacggaca cgtcgccgac gatctcagcg ccatcgatcg
     2341 tgaccgtgat gccgtcgagg gcgacgttca ccgagtggct ccggagcggc gcatgaggac
     2401 gaggaagaac ggagcaccca ccgccgcggt gaagatcccg accgggagct cgttgggccg
     2461 gtcgacgaca cgggcgagga gatcaacggc caccatgaac gaggcgccgc tgagcagcgc
     2521 cacggggagc agcctccggt ggtcgggccc gaccaggaat cggcacgcgt gcgggaccat
     2581 cagaccgacg aacccgatcc cgccggcgac ggccacggcg cacgcggtca gcagcgagga
     2641 gatgatcaac agttcgatgc ggaaccggtt gacgtcgacg ccgagtgccg aggcggagtc
     2701 gtctccggtc gacatcacgt tcagcgaccg cgccctcacc agcagccacg cggcggtcac
     2761 ggcgatgacg acggtcggga ctccgaggtc gtcccacgag gccgaggcga acgaccccag
     2821 gatccagaac atgaggccac gcagctcggc gggtcgggtg tagagctgga cgaagctggt
     2881 accggcggtg gcgaggtaac tgaccgcgac gccggccaga acgagccggg acggcccgac
     2941 gcggccgccg cgttgggcga gcacgaagac gacgaacagc gtcagcatcg ctgcgacgaa
     3001 cgcagcgccg gtcacgctca acccgcccga caccccggtg ccgtaggcga gcacgagcac
     3061 cgcgccgaac gacgcgccgg agctgacgcc gaggacatag ggatcggcga gtgcgttgcg
     3121 cccgagcacc tgaaggcacg caccggccac gctcagcgcg gcgccgacaa cggcggcgag
     3181 cagcacccgc ggtgtgcgga agtcccacac gatctggtcc tgcaccttcg tcggtgcggc
     3241 ggagagaacg ccgaggtgat gcccgacgac ggaccagacc tccttgaccg gcaacgtgac
     3301 cgtgccgatc gagatcccag cgaccagggc gacgaccaac ccgagcacga gcgcgacgag
     3361 cagggcgccg tagcccccac ctccgatgag gtgggggctc gaccgcgtac gggtctcagc
     3421 tcgcggcagc gatggcatcg gcgatcttct g
//
LOCUS       DAOSOA010000346         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_27529_length_3442_cov_5.003838, whole genome shotgun sequence.
ACCESSION   DAOSOA010000346 DAOSOA010000000
VERSION     DAOSOA010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27529_length_3442_cov_5.003838"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKY13_15410"
     CDS             complement(<1..675)
                     /locus_tag="PKY13_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT81382.1"
                     /translation="MFDTTVLTEMTAKIAGLDEHLYADDDLLAGVKAIEEARRHLDAA
                     EGALLGELDARGTTDVVSGHRTANWLAHETGIAPVTAKARVRLAVSLRLDLPETDRAV
                     REGRLCSDRARVMANAINDRIAAEFREMEASLIAESAQMTFAEWAQRVALVAALLDQD
                     GPEPSEDPNNNRLRLRGGRDGMKVTGVLVGDLAATTKAAVEQIADERWRQYRDDSERT
                     NGEIAVP"
     gene            796..978
                     /locus_tag="PKY13_15415"
     CDS             796..978
                     /locus_tag="PKY13_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81383.1"
                     /translation="MTAQLTLIDTADTHSWKIPEATRQIGRKGLAEARAILHSIPRRD
                     LDGDSGGTDQQHPAAA"
     gene            1024..2202
                     /locus_tag="PKY13_15420"
     CDS             1024..2202
                     /locus_tag="PKY13_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HOT81384.1"
                     /translation="MSDVVIIEAVRTPIGRRNGGLASVHPSDLLATVLSEVIVRSGID
                     PAEVGQVVGGCVSQVGEQSFNVARTAWLTAGLPQEVAATTVDTQCGSSQQATNLATAL
                     VAAGVVDVAVACGVEVMSRIPIGSNSSKKLGLGVPVPKSYFERYEFASQFEGAERIAD
                     KWGISRADCDAFGLQSQERGAAAWAEGRFDSQVVDVEAPDLDDEGKPTGTTHTVSRDE
                     GLRETTLEKLATLKPVARENGVHTAGTASQISDGAAAVLLMSAEKAAALGLTPRAKVL
                     DTAMVGVDPVMMLTGPIDVTRHLLARTGLTIGDIDLTEINEAFASVALAWLKELGGNP
                     ETTNPNGGAIALGHPLGATGAFLITKALGELERTDGTNALITMCCGGGLGTGTILQRV
                     "
     gene            2138..2761
                     /locus_tag="PKY13_15425"
     CDS             2138..2761
                     /locus_tag="PKY13_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOT81385.1"
                     /translation="MRSSRCAAAGGSERAPSCNASERQRPALRSATLAAIAVGLALGL
                     VAACSSHDPATLGAATDALEANAVVAKVVDGDTIDVTTGGESTTRIRMIGFNTPESVD
                     PRRPVECFGKEASKHLASLAPVGTSVRLERDAEERDRYGRTLAYVYRAADGLFLNLQM
                     VSDGYAHVLSIPPNVSYADRFRDAERSARTANLGLWASCPDDEASGG"
     gene            complement(2798..>3442)
                     /locus_tag="PKY13_15430"
     CDS             complement(2798..>3442)
                     /locus_tag="PKY13_15430"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOT81386.1"
                     /translation="HLTLNSEYDRYRWGPITHGPSLLDGDGGFPRTVEDSWDHADLDE
                     ARRECRAQIERAMLWGLDVTHLDSHMGTLQLRPEFFDIYLDMAIEFSLPLRMAGASVE
                     HTAGFPFRQLAADEGVLFPDSFVYVGGVGSRETIESVATSLRPGVTEVYVHPAVDTPE
                     LRAIADTWAERVDDLDLVAAGGPLAATLAEQGVALVGWREIRDLMRSGPVGRTA"
BASE COUNT          576 a         1240 c         1107 g          519 t
ORIGIN      
        1 aggcaccgcg atctcaccgt tggtgcgctc ggaatcgtcg cggtactgcc gccagcgttc
       61 gtcggcgatc tgctcgacag cggccttggt ggtcgcggca aggtcgccga cgaggacgcc
      121 ggtcactttc atgccgtcgc ggccgccacg gagtcggagc cggttgttgt tgggatcctc
      181 ggatggctcg gggccgtctt gatccaacaa ggcggcaacg agtgcgacgc gttgggccca
      241 ctcggcgaac gtcatctgag ctgactcggc gatcaacgac gcttccatct cccggaactc
      301 ggcggcgatc cggtcgttga tcgcgttcgc catcacccgc gcccggtccg aacacaggcg
      361 gccctcgcgc acggcccgat cggtctcggg cagatccaac cgcaacgaca ccgccaaccg
      421 cacccgcgct ttcgcggtga ccggtgcgat cccggtctcg tgtgccaacc agttcgctgt
      481 gcgatgaccg gacaccacgt cggtggtccc gcgggcgtcg agctcaccca acaacgcgcc
      541 ctccgctgcg tcgagatgac ggcgggcttc ttcgatcgct ttcacgccgg ccaacaggtc
      601 gtcatccgcg tacaggtgct cgtcgaggcc ggcgatcttc gccgtcatct cggtcagcac
      661 cgttgtatcg aacatgtgtt cgagtgtacc ggaagggtct gacactcaaa cgaggatcag
      721 ggcgtttcgt gccgattgag cacaactccc caggccacga atctcactgt catgggtgct
      781 cctcatactg gaaccatgac cgctcagctc accctcatcg acaccgccga cacccactcc
      841 tggaagatcc cggaggccac tcgccagatc ggccgcaagg gcctggccga agctcgtgcc
      901 atcctgcact cgatcccccg tcgtgacctc gacggcgact cgggtggcac cgaccagcag
      961 caccccgcgg ccgcctgatc acttcgccct ccccggccag cgccaccacc tacagttcac
     1021 gacatgtctg acgtcgtcat catcgaagcc gtacgcacgc ccatcggtcg ccgcaacgga
     1081 ggtctcgcat cggtccatcc gtccgacctc cttgcgaccg tgctgagcga agtcatcgtg
     1141 cgttccggca tcgatcccgc cgaggtgggt caggtcgtcg gcggctgcgt gtcccaggtc
     1201 ggcgagcagt cgttcaacgt cgctcgcacc gcatggctga ccgccggtct cccccaagag
     1261 gtcgcagcga cgacggtcga cacgcagtgc ggctcgtcgc agcaggccac caacctcgcc
     1321 accgcgctcg tcgcagcggg tgtggtcgac gtcgccgtcg catgcggtgt cgaggtcatg
     1381 agccgcatcc cgatcggctc caactcctcg aagaagctgg ggctcggcgt cccggtgccc
     1441 aagtcgtact tcgagcgcta cgagttcgcc tcccagttcg aaggggccga gcgcatcgcc
     1501 gacaagtggg gcatcagccg cgccgattgc gacgcgttcg gcctccaatc ccaagagcgc
     1561 ggggccgcag cgtgggccga gggtcggttc gacagccagg tggtcgacgt cgaggcgccc
     1621 gacctcgacg acgagggcaa gcccacgggc accacccaca ccgtcagccg tgacgagggg
     1681 ctgcgggaga ccacgctcga gaagctcgca acgctcaagc ccgttgcccg cgagaacggc
     1741 gtccacacgg ccggcacggc gagtcagatc tccgacggcg ctgcggcggt cctcctcatg
     1801 agcgccgaga aggcggcggc gctgggactg acaccacgag cgaaggtgct cgacacggcc
     1861 atggtcggcg tcgatccggt gatgatgctc acgggcccga tcgacgtgac ccgtcacctc
     1921 ctcgcacgca ccggcctcac catcggagac atcgacctca ccgagatcaa cgaggccttc
     1981 gcctcggtgg cactcgcctg gctcaaggag ctcgggggca atcccgagac gaccaacccc
     2041 aatggtggag ccatcgcgct cggccacccg ctcggcgcaa ctggcgcctt cctgatcacc
     2101 aaggcgctcg gcgaactcga gcgaacggac ggcaccaatg cgctcatcac gatgtgctgc
     2161 ggcggggggc tcggaacggg caccatcctg caacgcgtct gagcggcaac gccccgctct
     2221 ccgctccgca acactcgcgg cgatcgctgt cgggctggcc ctgggcctgg tcgcggcgtg
     2281 ctcgtcgcac gaccccgcca cgctcggggc cgccaccgac gcactcgagg cgaacgccgt
     2341 cgtcgccaag gtcgtcgacg gcgacacgat cgacgtcacc accgggggcg aatcgacgac
     2401 acgcatccgc atgatcggct tcaacacacc cgagagcgtc gacccgcgac gccccgtcga
     2461 gtgcttcggc aaagaggcct ccaagcacct ggcgtcgctc gctcccgtcg gaacgtcggt
     2521 ccggctcgaa cgcgacgccg aggagcggga ccgatacggc cgcacgctcg cgtacgtgta
     2581 tcgcgctgcc gacggcctgt tcctcaacct gcagatggtg agcgacgggt acgcgcacgt
     2641 gctctcgatc ccgccgaatg tgagctacgc cgaccgtttc cgcgacgccg aacgatcggc
     2701 tcgcaccgcg aacctcggcc tgtgggcatc gtgccccgat gacgaggcgt ctggcggcta
     2761 aggcgtccgc cagatgatac ggctgccgct tgagccctca ggcggtacgg ccgaccgggc
     2821 ccgagcgcat caggtcacgg atctcccgcc aaccgaccaa cgccacaccc tgctctgcca
     2881 gggtggccgc gagcggaccg ccggcggcga cgaggtcgag gtcgtccacc cgctctgccc
     2941 acgtgtcggc gatggcgcgc agctccggcg tgtcgaccgc cgggtgcaca tagacctcgg
     3001 tcactccggg acgaagcgac gttgcgaccg actcgatggt ctcgcgagag ccgacgccgc
     3061 cgacatagac gaacgagtcg gggaacagga ccccttcgtc ggcggcgagc tgacggaacg
     3121 ggaacccggc ggtgtgctcg accgacgccc ccgccattcg cagcggaagg gagaactcga
     3181 tggccatgtc gaggtagatg tcgaagaact caggacgcag ctggagcgtg cccatgtggc
     3241 tgtcgaggtg ggtcacgtcg agcccccaca acatcgcccg ttcgatctga gcgcggcact
     3301 cgcggcgcgc ctcgtcgagg tcggcgtggt cccacgagtc ctcgaccgtc cgcgggaagc
     3361 cgccgtcgcc gtcgagcagc gacgggccat gggtgatggg cccccaccgg taccggtcgt
     3421 actcggagtt cagcgtgaga tg
//
LOCUS       DAOSOA010000347         3433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_27611_length_3433_cov_5.049142, whole genome shotgun sequence.
ACCESSION   DAOSOA010000347 DAOSOA010000000
VERSION     DAOSOA010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27611_length_3433_cov_5.049142"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKY13_15435"
     CDS             complement(<1..367)
                     /locus_tag="PKY13_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81387.1"
                     /translation="MPVNALSVVSELAASQHNVFTRQQAAPRGAALAHPGSVASHSTS
                     GRLDGVDGYGGFASIDLTVVRPTTLRAPGVTTHRVHALDRCDVLMIGPVPCTNRARTL
                     CDLAGVVSADMLERALDDAV"
     gene            complement(490..1656)
                     /locus_tag="PKY13_15440"
     CDS             complement(490..1656)
                     /locus_tag="PKY13_15440"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOT81388.1"
                     /translation="MNTRDRLVEAAITATGLDDFGSDSWQEGLDLLLLSLDATADLND
                     IGVSVAEGGVVTDLSNRLLIEGWRRDHPEVATQRVDRPIIIVGQPRTGTTILFDLMAQ
                     DPTNRAPLSWEIERPVPAPRTATYGTDPRIAEAQAGFDLVESIIPGFTSFHELGAQLA
                     QEDVRIFTSDFRSMQFSLQFEVPEYNRWLLHEADMAPAYRWHRRFLQHLQSEHHADRW
                     LLKSPAHLWSLPSLLAEYPDAIVIQNHRDPLKVIASISALGASLREMTTDNFDVRTLA
                     AQYGDDIMVGLDRALQARADGLFAPGQVVDVRYQDFRHDPIATIAGIYDQIGLELTVD
                     TEARMRSFLAAHPGDQAGSLKRYSFADTGLDEDELRERARPYQEFFGVETEQLS"
     gene            complement(1719..2327)
                     /locus_tag="PKY13_15445"
     CDS             complement(1719..2327)
                     /locus_tag="PKY13_15445"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT81389.1"
                     /translation="MDGSEVATAGVGVRERKRLETRARIADAAAQLASEHGIADTTVD
                     EIAVAAGVGRATFFRYFESKELAIATGLSDAAIYVMSKALRDLPDDLDPIEAIRAAQA
                     ALGADFENDRAMFLEQALLSRSSTAMFAWTLHLYVDWELAIAEAVAPRFADLQPHDPR
                     PRMLGAMTMAASRLACDEWVAGGGSGDLPALVAKYLAAIRIE"
     gene            2400..3431
                     /locus_tag="PKY13_15450"
     CDS             2400..3431
                     /locus_tag="PKY13_15450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HOT81390.1"
                     /translation="MRAVQVTRHGPPGEVLAVNEVDVPEPGAGEVTIRVAAAALNFND
                     IDRCRGKLVSVTKQPPFTLGMDVCGVVTGAGAGAEQWIGRRVVGISKDALGGIAEYTV
                     APAVGVFDAPESLSDEEAAAFLIPFHTTHLALFRRGQLTAGETLLVHSGASGLGTAAI
                     QLGVSAGARVFATVSSPEKAELCRSLGADLVIDHTQEDIVEAVLGATDDVGADVICDL
                     AGGSFVPKSWQCVAREGRYLAVGFTDDDENGMTGRPLRMASIGNFSVVGVLGAWVDDL
                     DPGMRRFGFNPFTRADGDEVHQALVDLIAAGRIRPHVGRVVAMDEAGAALDDHEQRRS
                     IGRTVVRID"
BASE COUNT          523 a         1130 c         1243 g          537 t
ORIGIN      
        1 gcaccgcgtc gtcgagtgcc cgttccaaca tgtccgcact gacgactccg gcaaggtcgc
       61 agagcgtccg ggcgcggttc gtacacggca ccggtccgat catgaggacg tcacaccgat
      121 cgagcgcgtg cacacggtgg gtcgtgactc ccggcgcacg gagcgtggtg gggcggacca
      181 cggtcaggtc gatcgacgcg aacccgccgt acccgtccac gccgtcgagg cgaccactgg
      241 tcgagtggga ggccaccgaa cccggatggg cgagcgcggc gccgcgggga gcagcctgct
      301 ggcgggtgaa gacgttgtgt tgagaggctg cgagctcgga cacaacgctc aaggcgttga
      361 ccggcatgtc gccatcatcg cgatcatcgc tgacagtgag ttcaccgcgg acgacggacc
      421 gttccgggcg cgccagcggt acggatgtga cccgctggcg cgcccgaaac cgggaacccg
      481 ggcttcgggt caggacaact gctcggtctc caccccgaag aactcctggt acgggcgggc
      541 gcgctcacgc agctcgtcct cgtcgaggcc ggtgtcggcg aacgagtagc gcttgaggct
      601 gcccgcctgg tcaccgggat gcgcggcgag gaacgatcgc atacgcgcct cggtgtcgac
      661 cgtgagttcc aggccgatct ggtcgtagat accggcgatc gtcgcgatcg ggtcgtggcg
      721 gaagtcctgg tagcgcacat ccacgacctg gcccggagcg aacagcccgt ccgcccgtgc
      781 ctgcaacgcc cggtcgaggc ccaccatgat gtcgtcgccg tactgggcgg ccagggtgcg
      841 cacgtcgaag ttgtcggtcg tcatctcccg caggctggcc cccaacgcgc tgatcgaggc
      901 gatgaccttg agcgggtcgc ggtggttctg gatgacgatg gcatcggggt actcggcgag
      961 cagggacggg agtgaccaca ggtgcgccgg cgacttgagc agccatcgat ccgcgtggtg
     1021 ctccgactgc aggtgctgca ggaagcgccg gtgccaccgg taggccggcg ccatgtccgc
     1081 ctcgtggagg agccaacggt tgtactcggg cacttcgaac tgcagcgaga actgcatcga
     1141 ccggaagtcg ctcgtgaaga tccgcacgtc ctcctgcgcg agttgggcgc ccagttcgtg
     1201 gaacgaggtg aaccccggga tgatcgactc gacgagatcg aacccggcct gcgcctcggc
     1261 gatgcggggg tcggtcccgt aggtggcggt tcgcggcgcc ggcaccgggc gttcgatctc
     1321 ccagctgagc ggcgcgcggt tggtcgggtc ctgggccatc aggtcgaaca ggatcgtggt
     1381 gccggttcgg ggttgcccga cgatgatgat cggtcggtcg acccgctggg tggccacctc
     1441 gggatggtca cggcgccatc cctcgatcag cagccgattg gacaggtcgg tcacgacccc
     1501 gccctcggcg acgctgacgc cgatgtcgtt gaggtcggcg gtggcgtcga gcgacaacag
     1561 gaggaggtcg aggccttcct gccaactgtc ggacccgaag tcgtcgagtc cggtcgcggt
     1621 gatcgccgcc tcgacgagtc ggtcgcgtgt gttcatttcg tttccccctg gttgatgtcg
     1681 ttcgtgccgt gtgggcgctg tccccccgga tcgcgccgtc actcgatccg gatcgccgcc
     1741 agatacttcg cgaccaacgc cggcaggtcg ccgctgccgc cgccggcgac ccattcgtcg
     1801 caggcgagcc gcgaggccgc catggtcatg gccccgagca tgcgggggcg cgggtcgtgg
     1861 ggctgcaggt cagcgaagcg gggagcgacc gcctcggcga tcgcaagctc ccagtcgacg
     1921 tacaggtgga gcgtccacgc gaacatcgcc gtggacgagc ggctgagcaa cgcctgctcg
     1981 aggaacatcg cacggtcgtt ctcgaagtcg gccccgagcg ccgcttgcgc cgcccggatc
     2041 gcttcgatcg ggtcgaggtc gtcgggcagg tcgcgcaggg ccttcgacat cacgtagatg
     2101 gcggcatcgg agagtccggt ggcgatcgcc aactcctttg actcgaagta gcggaagaac
     2161 gtggcccggc cgacaccggc ggcgacggcg atctcgtcga cggtggtgtc ggcgatgccg
     2221 tgttcggacg cgagctgcgc cgcggcgtcg gcgatccggg cgcgggtctc gaggcgtttg
     2281 cgttcccgca ccccgacgcc ggctgttgcg acctcgctgc catccatcga ccgaagcgta
     2341 cccacaaagt tgagacggag tatcgttttg gtgtcgtcag cgaactcagg gggaagcgta
     2401 tgcgagctgt gcaggtgacc cgtcacggac ctcccggcga ggtgctggcg gtgaacgagg
     2461 tcgatgtccc cgaaccggga gcgggcgagg tgacgatccg ggtcgcagcg gcggcgttga
     2521 acttcaacga catcgaccgg tgtcggggga agctcgtctc ggtcaccaag cagccgccct
     2581 tcacgctcgg catggacgtg tgcggggtcg tgaccggcgc cggtgcagga gcggagcagt
     2641 ggatcggacg acgggtcgtc gggatttcga aggatgcgct cggcggcatc gccgagtaca
     2701 ccgtcgcccc ggctgtcggg gtgttcgacg cgcccgagtc gctcagcgac gaagaggcgg
     2761 cagccttcct catcccgttc cacacgaccc acctcgccct cttccgacgc ggtcagctga
     2821 cggcagggga gacgctgctc gtccactccg gggccagcgg gctcggcacc gccgccatcc
     2881 agctcggggt cagtgccggc gcccgggtgt tcgccacggt cagcagcccc gagaaggccg
     2941 agctgtgccg gtcgctcggc gccgacctgg tgatcgacca cacccaggag gacatcgtcg
     3001 aagccgtgct cggcgccacc gacgatgtgg gcgccgacgt gatctgcgac ctggccggcg
     3061 gctcgttcgt gccgaaatcg tggcagtgcg tggcacgcga gggccggtac ctcgccgtcg
     3121 ggttcaccga cgacgacgag aacgggatga ccggccgccc gctccgcatg gcgagcatcg
     3181 gcaacttctc ggtcgtcggg gtgctcggcg cctgggtcga cgacctcgat ccgggcatgc
     3241 ggcggttcgg gttcaacccg ttcacccgcg ccgacggcga cgaggtccac caagcgttgg
     3301 tcgacctgat cgccgccggc aggatccggc cccacgtcgg ccgggtcgta gcgatggacg
     3361 aggccggcgc cgcactcgac gaccacgaac agcggcgctc gatcgggcgc accgtcgtcc
     3421 ggatcgactg agc
//
LOCUS       DAOSOA010000348         3394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_28032_length_3394_cov_4.598083, whole genome shotgun sequence.
ACCESSION   DAOSOA010000348 DAOSOA010000000
VERSION     DAOSOA010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28032_length_3394_cov_4.598083"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PKY13_15455"
     CDS             <1..1022
                     /locus_tag="PKY13_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81391.1"
                     /translation="GRWVAFTSSATDLLPGSLGDGAFLRDRWTDTTIRLGSDVAGRLT
                     PTRMSHDGNFVAGTGIDGAVSFTWIHDQRSHHTVKLAPEAGRQPAEILGINNDGYFIA
                     RDGASIAGAARLNRAGRLLGDGTFTPLSVTAKGSRVFDALTPSLQYALATRDVGTAPW
                     GAQGDTLRIDLTTGSMTSFVDTFPGADGRLSRIRSTAISPNGRYVAMVFQMPIGFAIR
                     IWDAPAGAYVDHHVPDGEDGRDPVIRAVLDDGRIIYDTVTNAVFFADPVRHPEREPRR
                     LTVKFDDGSPVRIRHDEGFRASSQVATDIDRVLICPLDPMSSFALTFQEHCYLKPFPP
                     SPLAG"
     gene            1090..2256
                     /locus_tag="PKY13_15460"
     CDS             1090..2256
                     /locus_tag="PKY13_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HOT81392.1"
                     /translation="MPNHGDWQLGIYFGGLEGVVPALPMSFDALAAAAEAALTPEIWS
                     YVSGGAGNEHTQRANVAAFERWGLMPRVLRGASQREVGVDLFGHHYSTPLMLAPVGVI
                     GLCEAGGQGDLSTARAAASTGVPMVASTLMEVPMEDVAAQFGDTPGWFQLYPPSDREL
                     CESFIRRAEAAGYTAIVVTLDTLTLGWRPRDLTLASFPQLKGLCLANYFSDPVFRSRL
                     ERTPEEDIETATGLWAFLFGNPGMTWDDLDWIRSVTDLPLLIKGVNSPEDTRTAIDHG
                     VDGIYCSNHGGRQANGGIPAIDLLADVVAAAGDAPVIFDSGIRSGADIVKALALGATA
                     VGIGRPYAYGLAAGGQAGVEHVLKCLLAELDLIMAVDGYASLAELTPDALRRVG"
     gene            complement(2403..3197)
                     /locus_tag="PKY13_15465"
     CDS             complement(2403..3197)
                     /locus_tag="PKY13_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT81393.1"
                     /translation="MELSASNARIKQLRRLSGRRSSRSEEGVFVIEGAVLVREAINAG
                     VDIDAIFVDVARGGLVAEFGELHPDVWTVAEGVLASVLPAVSAQPVGAVASTSLERAV
                     DDLVVAAVDSGRPVLVLVEVADPGNVGTLLRAAESSGCGGVVLAGSGVDLYNPKVVRA
                     SAGSLFRLPVATAPDVGAAQVAIAELGMPMIAAVARDGVPHLEATLGGPVAIVLGNEA
                     HGLSDEVVAACDAAVTITMDGPAESLNVAMAGTVLCFEALRRRRSD"
     gene            complement(3233..>3394)
                     /locus_tag="PKY13_15470"
     CDS             complement(3233..>3394)
                     /locus_tag="PKY13_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT81394.1"
                     /translation="NDISYSRFINGLKKAEIEIDRKSLADLAVTDPAAFAGLVAAAKA
                     GLESAAAAS"
BASE COUNT          520 a         1229 c         1137 g          508 t
ORIGIN      
        1 cgggtcgatg ggtcgcgttc acgtccagcg ccaccgacct ccttcccggc agtctcggtg
       61 atggcgcctt cctccgcgac cggtggaccg acaccacgat ccggctcggc agcgacgtcg
      121 ccgggcggct cacgcccacg cggatgtccc acgacggcaa cttcgtcgcc gggacgggaa
      181 tcgacggagc ggtgagcttc acgtggatcc acgatcaacg gtcccaccac accgtgaagc
      241 tggcacctga ggcaggccgg caacccgcgg agatactcgg catcaacaac gacgggtact
      301 tcatcgcccg agacggcgcg tcgattgccg gcgccgcccg gctgaaccgg gccggacgcc
      361 tgctcggcga cggcacgttc accccgttga gcgtgacggc gaagggcagc cgcgtcttcg
      421 atgcgctcac cccgtcgctc cagtacgccc tcgcgacgcg tgacgtcggg accgccccgt
      481 ggggagcgca gggagacacc ttgcgcatcg atctgaccac cggctccatg acgagcttcg
      541 tcgatacgtt ccccggcgcc gacggccggc tcagccggat ccgctcgaca gcgatctcac
      601 cgaatgggcg ctacgtggcg atggtgttcc agatgccaat cggcttcgcc atccggatct
      661 gggacgcccc ggcgggcgcc tacgtcgacc atcacgtccc cgacggcgag gacggccgcg
      721 atccggtgat ccgcgccgtg ctcgatgacg ggcgcatcat ctacgacacc gtgaccaacg
      781 cggtgttctt cgcggatccg gtgcgccacc ccgagcggga accccgtcgg ttgaccgtga
      841 agttcgacga cgggtcgccc gtgcgcatcc gccacgacga agggttccga gcctcgtccc
      901 aggtggcaac cgacatcgac cgcgtgctga tctgcccact cgaccccatg agctcgttcg
      961 ctctcacgtt ccaggagcac tgctacctca agcccttccc gcccagcccc ctcgctggtt
     1021 gacccaggcc gactcgttca ccgagcagaa tggtcggtcg acgagctcga tcaacacggg
     1081 ggacatggcg tgccgaacca cggtgactgg cagttgggga tctacttcgg aggcctcgag
     1141 ggggtcgttc ccgcgttgcc gatgagtttc gacgcgctcg cggcggcggc cgaggcggca
     1201 ctcacccccg agatctggtc ctatgtgagc ggcggggccg ggaacgaaca cacccagcgc
     1261 gccaacgtcg cggcgttcga gcggtggggg ctcatgccac gggtcctgcg cggggcgtcg
     1321 cagcgcgagg tgggggtcga tctgttcggt caccactact cgacgccgct gatgttggcg
     1381 cccgtcgggg tcatcggact ctgcgaggcc ggtggccagg gtgatctgtc caccgcccgg
     1441 gcagccgcgt cgaccggcgt gccgatggtg gcctccacgc tgatggaggt accgatggag
     1501 gacgtcgccg cccagttcgg cgacaccccc ggttggttcc agctctatcc gccgagcgac
     1561 cgggaactct gcgagagctt catccgccgc gccgaggcgg ccggctacac ggcgatcgtc
     1621 gtcaccctcg acaccttgac cctgggatgg cgaccacgcg acctgaccct cgccagcttt
     1681 ccgcagctca agggcctctg cctcgccaac tacttcagcg atccggtgtt ccgctcgcgc
     1741 ctggaacgta cccccgagga ggacatcgag accgccacgg gcctctgggc gttcttgttc
     1801 ggcaaccccg gcatgacgtg ggacgacctc gactggatcc gatcggtcac cgacctgccg
     1861 ctcctgatca agggcgtgaa ctctcccgag gacacgcgca cggcgatcga ccacggcgtc
     1921 gacggcatct actgctccaa ccacggcggc cgtcaggcca acggcggcat ccccgccatc
     1981 gacctgcttg cggacgtggt cgcggcagcg ggcgacgccc cggtgatctt cgactcgggc
     2041 atccgctcgg gcgccgacat cgtcaaggcg ctggcactcg gggccactgc ggtcggaatc
     2101 ggacgcccat acgcctacgg tctcgcggcc ggaggccagg ccggtgtcga gcacgtgctc
     2161 aagtgcctgc tggccgaact cgacctcatc atggcggtcg acggctacgc gagcctcgcc
     2221 gaactcactc ccgacgcgct gcgccgcgtc ggctgacccg cgcgctcacc cgtcgagtcg
     2281 gcgccgcagt tcagtcgctg cgcctccggc gcagttcagt cgcttcgcct ccggcggcgc
     2341 cgcagttcag tcgctgcgcc tccggcgcag ttcagtcgct tcgcctccgg cggcggcgca
     2401 gttcagtcgc tgcgcctccg gcgcagtgcc tcgaagcaga ggacggtgcc ggccatggcg
     2461 acgttgagcg actcggccgg accatccatc gtgatggtca ccgcagcgtc gcacgcggcg
     2521 acgacctcgt cggacaggcc gtgggcttcg ttgccgagca caatcgccac cggacctccg
     2581 agcgtcgcct cgaggtgggg cacaccgtcg cgggcgaccg cggcgatcat cggcattccg
     2641 agctcagcga tcgcgacctg cgcggcgccg acgtcaggcg ccgtcgcaac cggcagccgg
     2701 aagagcgaac cggctgacgc ccgaacgacc ttggggttgt agaggtcgac gcccgagccg
     2761 gcgagcacaa caccgccaca gcccgagctc tcggctgcac ggagcagggt gccgacgttg
     2821 ccggggtcgg cgacctcgac gagcaccagc acggggcggc cggagtcgac cgccgccacg
     2881 acgaggtcgt cgacggcgcg ttcgagcgag gtcgacgcga ccgcgccgac cggttgggcg
     2941 ctgacggccg gcaggaccga ggcgagcact ccctcggcga cggtccagac gtcgggatgg
     3001 agttcgccga actcggcgac gagcccccca cgggcgacat cgacgaagat cgcatcgatg
     3061 tcgacgccgg cgttgatcgc ctcgcgtacc agcacggcgc cctcgatcac gaagacgccc
     3121 tcctcggaac gcgaactgcg gcgccccgag aggcgccgca gctgcttgat ccgagcgttc
     3181 gaagcgctca gttccatcgc accggctccg ccggagtcca tcggtgacga gatcagctcg
     3241 cggccgcggc gctctcgagg ccggccttgg cggcggcgac gagaccggcg aatgccgccg
     3301 ggtcggtcac ggcgaggtcg gcgagcgact tacggtcgat ctcgatctcg gccttcttga
     3361 gcccgttgat gaaccggctg tagctgatgt cgtt
//
LOCUS       DAOSOA010000349         3381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_28156_length_3381_cov_5.249248, whole genome shotgun sequence.
ACCESSION   DAOSOA010000349 DAOSOA010000000
VERSION     DAOSOA010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28156_length_3381_cov_5.249248"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    54..153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            405..605
                     /locus_tag="PKY13_15475"
     CDS             405..605
                     /locus_tag="PKY13_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81395.1"
                     /translation="MTTTLLVVALVASSCELSLNNVDCSGIDQPDMGGLVCLGANVFA
                     LQAGAALIIAALAALLGGAFPG"
     gene            643..1092
                     /locus_tag="PKY13_15480"
     CDS             643..1092
                     /locus_tag="PKY13_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81396.1"
                     /translation="MAELQPPELAALVDFPERPRVENWSLRAALTRYAQPQPMRVSRL
                     LDVVRRIEAAFAADLAVLRSDGPELWAALGSGTDLDGRSQLLGVLAAAQQIDRLADDL
                     AAWAVRRDTVRPDDAVDVTIADVTARLDALGVVEPVDTRPPPSRRRG"
     gene            1148..1222
                     /locus_tag="PKY13_15485"
     tRNA            1148..1222
                     /locus_tag="PKY13_15485"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Asp,seq:gtc)
     gene            1285..1360
                     /locus_tag="PKY13_15490"
     tRNA            1285..1360
                     /locus_tag="PKY13_15490"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1318..1320,aa:Phe,seq:gaa)
     gene            complement(1819..2610)
                     /locus_tag="PKY13_15495"
     CDS             complement(1819..2610)
                     /locus_tag="PKY13_15495"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT81397.1"
                     /translation="MNASNGPETEPEPARTGSQSIERALSVLGFFVEERSLSVTEISR
                     RLGLSVSTAHRITRALVRAGYLDQDTVSERYRLGRMVAVLGRAATDSLNLADAQAVLD
                     TAAAECGESVSLGVIDDRDVLILATAESSQPLRFDRATGSRTPVHASAIGKAILAFAP
                     RDRWRGPSTTLPAFTERTIVAGVDLEGQLEQIRSDGVALNLEERYVGVCAVAAPVLDA
                     AGVARAGVGFQVPLARFGADRQRELSERAVTLAAELAEALPLDRL"
     gene            2716..3189
                     /locus_tag="PKY13_15500"
     CDS             2716..3189
                     /locus_tag="PKY13_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81398.1"
                     /translation="MPHDDALPGLATARELHPSSDRADQPIGIDAIESIALGLAAVVG
                     TPTEQPARGEVHRHRVIATAAYDAWIVTVGPQTAIEPHDHDGSIGVIAVTDGHLIEFG
                     LDDDAQRRSRLRHLIVGDRTEIGITHRHSLANPDLVPVTTVQVFSPPLGHDRESH"
     gene            complement(3245..>3381)
                     /locus_tag="PKY13_15505"
     CDS             complement(3245..>3381)
                     /locus_tag="PKY13_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT81399.1"
                     /translation="PAYHDETNYLRVYVANLRRKLEATPSQPDHLITEPGMGYRFIAD
                     "
BASE COUNT          513 a         1133 c         1122 g          513 t
ORIGIN      
        1 aggacatcgc cctttcacgg cgacagcacg ggttcgaatc ccgttggggg tacnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncagccgt cggttgcacc ggaggatgtc
      181 gaccctccgg gccgacggtt cgaagcggtg ccgttggggg taccagccgt cggttgcacc
      241 ggaggatgtc gaccctccgg gccgacggtt cgaagcggcg ccgttggggc accactcgct
      301 ggtggggtgc acgttccgcc gctcgcgacg agccgacccg ataggttccg tgcatcgacg
      361 gcgccgggga gggcacgatg acgcggaacc gcaggatgat ggtggtgaca acgaccctgc
      421 tcgtggtcgc tctggtcgcg agctcgtgtg agctcagttt gaacaacgtc gactgcagcg
      481 gaatcgacca gcccgacatg ggcggcctcg tgtgcctcgg agcgaacgtg ttcgccctgc
      541 aggccggcgc tgccctcatc atcgcggccc tcgccgcact gctcggcggc gcgttcccgg
      601 gttgataccg cccgggagcc ggtcggctgc catgatctcg gggtggccga actgcaaccg
      661 cccgagctcg ccgcactggt cgatttcccg gaacgacctc gggtcgagaa ctggagcctg
      721 cgggccgcgc tcacccgcta cgcgcaaccg caaccgatgc gggtgagtcg gctgctcgac
      781 gtcgttcgtc ggatcgaagc cgcgttcgcc gctgatctcg ccgtgctccg atccgacggc
      841 ccggagttgt gggcggcact gggctcgggg accgacctcg acggccgatc gcagctcctc
      901 ggcgtgttgg ccgcagctca gcagatcgac cgacttgccg acgacctcgc ggcctgggcg
      961 gtgcgccgcg acacggttcg gcccgacgac gcggtcgacg tgaccatcgc cgacgtcacc
     1021 gctcgactcg acgcgttggg ggtcgtcgaa ccggtcgaca cccggccgcc gccgagccgt
     1081 cgacggggct gatcgaattt cgattccacg agtgcgcgcg ggtcccgcta tgctctcctc
     1141 cggttctggt cccgtggtgc agtttggagt gcacgccgcc ctgtcaaggc ggaggtcgcg
     1201 agttcaaatc tcgtcgggac cgccgcaccc tcgcctcggc gagggtgtct gcgttgggga
     1261 ggccttcggg tcgccccacc acctggtcgg gtagctcagt aggcagagcg accgcctgaa
     1321 aagcggtagg tcaccggatc gacgccggtc ccgaccacca gcgaagcccc cgagcaatcg
     1381 ggggcttctg tggttttcga atgagcgatg ctgccgactg ggccgaacga accagattcg
     1441 gtttcgattt gtacagcccc cggttggttc gaccgatacc tctggtgcgg cacgtttggc
     1501 gggtcgcagt gggcatggac gcccactcct ctacggcttt tcaggagtga gcgtgtccag
     1561 gttgaacggg ctgcggaatc gtcgcggcca ggttgtcttg ttgtcccttg ccatcgccgc
     1621 cagcgcggtg gccgccggtt gcggagccaa ctcggccccg agtgtcgaac gcgacgtggt
     1681 tcgctccgac cgagtgacca ccacgaccag cgctcccgtc gcagcgcaga tcggcgaggc
     1741 agtggtcatc gaacgtggcg gagaacagca cgtgcggaac ggctgagcct ccccggaccg
     1801 agcagcgtca gagcgtggtc agagtcggtc gagcggaagc gcttcggcca gctcggcggc
     1861 cagcgtcacg gcgcgttcgg acaactcccg ttgacgatcg gccccgaagc gggccagcgg
     1921 cacctggaag ccgacgccag cacgggcgac gccagccgca tcgagcaccg gagcggcgac
     1981 agcacacacg ccgacgtacc gctcctcgag gttgagcgcg acgccatcgg agcggatctg
     2041 ttcgagctgc ccttcgaggt cgacgcccgc cacgatcgtc cgctcggtga aggccggaag
     2101 ggtggtcgac gggccacgcc agcggtcgcg cggggcgaag gcgaggatcg ccttgccgat
     2161 ggccgaggca tgcaccgggg tacggctccc cgtggcccgg tcgaagcgga gcggctgact
     2221 cgactcggcg gtggcgagga tcaggacgtc ccggtcgtcg atcacgccga gactgacgga
     2281 ctcgccgcac tccgcggcgg cagtgtcgag caccgcctgc gcgtcggcca ggttgaggct
     2341 gtcggttgcg gcacgaccca gcaccgccac catgcgcccc aggcggtagc gctcggacac
     2401 cgtgtcctgg tcgaggtacc cggcccgaac gagggcacgg gtaatgcggt gcgccgtgga
     2461 cacgctgagg ccgagccgtc ggctgatctc ggtgacgctc aaggagcgct cctcgacgaa
     2521 gaaccccagc accgacaggg cgcgttcgat ggactgggag ccggttcggg ccggttccgg
     2581 ctcggtctcg gggccgttgc tcgcgttcac gcagcaccga gtctgccatg gtcccgggca
     2641 cctccgaccc agaccgaccg tcctccctcc taattacgac ttgttcggtc gggtattcca
     2701 cgatacggtc gcctcatgcc acatgacgac gcactcccgg gcctggcaac ggccagggaa
     2761 ctgcacccga gcagcgatcg ggcggaccag cccatcggca tcgacgccat cgagtcgatc
     2821 gccctcggac tcgccgccgt cgtcggcacg ccaaccgagc agcccgcccg cggcgaggtc
     2881 caccggcatc gggtgatcgc cacagccgcc tatgacgcct ggatcgtcac cgtggggcca
     2941 cagaccgcca tcgaacccca cgaccacgac ggatcgatcg gcgtgatcgc ggtcaccgac
     3001 gggcacctga tcgaattcgg cctggacgac gacgcccagc ggcgatcacg gctccgccac
     3061 ctgatcgtcg gggaccggac cgagatcggc atcacgcacc gacacagtct cgccaacccc
     3121 gacctcgtcc cggtgacgac cgtgcaggtg ttctcacccc cgctcggtca cgatcgcgaa
     3181 tcacactgac gtgtcgtcgc ggcaactcgc ctcctacctc cagaactgac gaccgcgacg
     3241 ccgctcagtc agcgatgaat cggtagccca tccccggctc ggtgatgaga tgatcgggct
     3301 gggacggggt cgcctcgagc ttgcgccgga ggttggccac gtagacccgg agatagttcg
     3361 tctcgtcgtg gtacgccgga c
//
LOCUS       DAOSOA010000350         3376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_28219_length_3376_cov_5.470942, whole genome shotgun sequence.
ACCESSION   DAOSOA010000350 DAOSOA010000000
VERSION     DAOSOA010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28219_length_3376_cov_5.470942"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PKY13_15510"
     CDS             <1..916
                     /locus_tag="PKY13_15510"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOT81400.1"
                     /translation="GLGELTNTPSLIDGPWPGELSALMLDTESTFNGLVARYAGSADQ
                     AEKILSNRFYRNISGALSGTQEYMATEKLYELSSDGEFDLIVVDTPPTRNALDFLEAP
                     GRLAKFLGHPLYRMVTAPTRGIVKAVNMAAQPVLRSVGKIVGGEVIADAIAFFAAFDG
                     MEEGFKQRAQMVETLLADDRTAFVLVASPRRDTVEEAAYFADRLADMRLSVRAVVVNR
                     IYPFFGSGLADELAVAARSADADGPLADHLRNLAELDDIARAEREHLAPLAERVAPAP
                     LVEVPLLPFEVHDLTSLTALGDYLFGRA"
     gene            complement(947..1291)
                     /locus_tag="PKY13_15515"
     CDS             complement(947..1291)
                     /locus_tag="PKY13_15515"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT81401.1"
                     /translation="MVDVEVVREEAATICRPVGELDAFSVADFREHLGGLAGDAAVII
                     DLSGVPFMDSAGLGALIGGIRKIREAEGEVVVVCDRPAVLRLLHTTRFDRMVTVVALV
                     DDAHAELATPSS"
     gene            1476..1880
                     /locus_tag="PKY13_15520"
     CDS             1476..1880
                     /locus_tag="PKY13_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT81402.1"
                     /translation="MRNVLLATDSDEVFIEIDAALGSADTQVHRIRAGREVRAAVIEL
                     DPDLVILDLQIGSMGGIAACLDLRLEEGAGRLDPQSVLLLLDRDADVFMARRSGADGW
                     LVKPLDARRITAATTAVIAGDFFAEGPELVET"
     gene            1962..2035
                     /locus_tag="PKY13_15525"
     tRNA            1962..2035
                     /locus_tag="PKY13_15525"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1996..1998,aa:Pro,seq:cgg)
     gene            2222..2863
                     /locus_tag="PKY13_15530"
     CDS             2222..2863
                     /locus_tag="PKY13_15530"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOT81403.1"
                     /translation="MPICASNPRRGQRAHRRRARPHRRVGERGATLVEGALVAPLFFL
                     LIFSILEFGLVFRQYLTMSTADRNGTRAASAAGNAAQADYVTLQAIKKSSTAIKTSEI
                     TAIVIFKASGPAASINDVALQACLTASVAGVCNRYTAADLARPVGDFGNCSLGSASPD
                     RFWCPTTRVVAASGPPDYIGVYIATRYQAVTKIFGKSYNLSEQTVYRMEAQTR"
     gene            2908..>3376
                     /locus_tag="PKY13_15535"
     CDS             2908..>3376
                     /locus_tag="PKY13_15535"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOT81404.1"
                     /translation="MALVVPLLLTLMLGIFEVGMMFRSDIAIANATRTAARTGSTAGK
                     APTADFQILSSLSAALGSVKNATVNYVVIYKVTGSTNAVPAACTSAAAIAAHGNAANF
                     CNTYSAADLSAILANPVSAQTSYGGSCAGAGKDTKWCPTTRVVDQGATNGPDYL"
BASE COUNT          556 a         1242 c         1051 g          527 t
ORIGIN      
        1 gggcctcggc gaactcacga acactccgtc gttgatcgac ggcccgtggc ccggtgagtt
       61 gtcggcgctc atgctcgaca ccgagtcgac gttcaacggg ctcgtcgccc gctacgccgg
      121 ctccgccgac caggccgaga agatcctctc caaccgtttc taccgcaata tctccggcgc
      181 gctctcggga acccaggagt acatggcgac cgagaagctc tacgaattgt cgagcgacgg
      241 ggagttcgac ctcatcgtgg tcgacacccc gccgacccgg aacgcgctcg acttcctcga
      301 agcacccggt cggctggcca agttcctcgg ccacccgctc taccgaatgg tgaccgctcc
      361 gacccgcggc atcgtcaagg ccgtcaacat ggccgcgcaa cccgtccttc ggagcgtggg
      421 caagatcgtc ggtggcgagg tcatcgctga tgcgatcgcc ttcttcgccg ccttcgacgg
      481 catggaggag gggttcaaac aacgagccca gatggtcgag accctcctgg ccgacgatcg
      541 aaccgccttc gtgctggtcg catcacctcg gcgcgacacg gtcgaggagg ctgcgtactt
      601 cgccgaccgt ctcgccgaca tgcggctctc ggtgcgagcc gtcgtcgtca accgcatcta
      661 cccgttcttc ggcagcggcc tcgccgacga actcgccgtg gcagccaggt cggccgacgc
      721 cgacggcccg ctcgccgacc acctgcgcaa cctcgccgag ctcgacgaca tcgcccgcgc
      781 cgaacgcgag cacctggcgc cgctggccga acgcgttgcc cccgcgccgc tggtggaagt
      841 accgctgttg cccttcgagg tgcacgacct cacgagcctc accgcgctgg gcgactacct
      901 cttcggtcgc gcctgaccgt cgcacccgac tccgggccga tcgggttcag gaactcgggg
      961 tggcgagctc tgcgtgggcg tcgtcgacga gggccacgac cgtgaccatc cgatcgaacc
     1021 gggtggtgtg gagcagtcgc aggactgcgg gccgatcgca cacgacgacg acctcaccct
     1081 cggcctcgcg gatcttgcgg atgcccccga tgagcgcacc gagccccgcc gaatccatga
     1141 acgggacacc ggacagatcg atgatgactg cggcatcacc ggcgagaccg ccgaggtgct
     1201 cgcggaagtc ggcgacgctg aaggcatcga gctcgccgac gggccggcag atcgttgccg
     1261 cttcctctcg aaccacctcg acgtcgacca ccgtttgctc cttccggccc tctcgggatc
     1321 cggcgcttcc agccggactg cccggctcat acccacgtga tgtccggggc gagccacatg
     1381 gtatcgacgc acccccgaca atcaacagca gctcccccga agtgggcatc gccgccggtt
     1441 ggggcgaggg gcatcaccga agtacccttc cggacgtgcg aaacgtgctg cttgccaccg
     1501 actccgacga ggtgttcatc gagatcgacg ccgccctcgg gtcggcggac acccaggtgc
     1561 accggatccg ggccggccgt gaggtgcggg ccgcggtgat cgagctcgat cccgacctcg
     1621 tgatcctcga cctgcagatc ggttcgatgg gcggcatcgc cgcctgcctc gatctccgcc
     1681 tcgaggaggg tgccggtcgc ctcgatccgc agtcggtgct cctgttgctc gaccgcgacg
     1741 ccgacgtctt catggcacgg cgctcggggg ctgacggttg gctcgtcaag ccgctcgacg
     1801 cccgccgcat caccgccgcc acgaccgccg tcatcgccgg cgacttcttc gccgaggggc
     1861 ccgaactcgt cgagacctga gaccccggac cgccctacct gggccgccgt gcgaatcgca
     1921 ttgcggccga ggggcaccga ggcggtaaca tcgcctctcc acgggttgtg gctcagcttg
     1981 gtagagcgct gcgttcggga cgcagaggtc gggagttcaa atctccccaa cccgactgcg
     2041 aggaccgatc gaggtcgtcg tgagagttcg gcgccgggtt ggcgtttccg catcacggag
     2101 acccgtcagg gcgtcggatg gctgctccgt ctgcagcacg gaccagcccg aagcgccgcc
     2161 gcgatgggta ttttcgcccc caactggtta ggtactttga cctttctgcc gataggcaac
     2221 catgccgatc tgcgcctcga acccccgacg tgggcagcgc gcacatcgac ggcgtgcccg
     2281 gccccaccgg cgcgttggtg aacggggcgc caccctggtc gaaggggccc tcgtcgctcc
     2341 cctgttcttc ctcctgatct tctcgatcct cgagttcggc ctcgtcttcc ggcagtacct
     2401 cacgatgtcg accgccgacc gaaacggcac ccgcgccgcg agcgccgccg gcaacgctgc
     2461 tcaggccgac tacgtcacgc tgcaggccat caagaagtcg tcgaccgcga tcaagacgag
     2521 cgagatcacc gcaatcgtga tcttcaaggc ctccggtcca gctgcatcga tcaacgacgt
     2581 cgcgctccag gcgtgcctga cggcatcggt ggccggggtc tgcaaccggt acacggcggc
     2641 cgacctcgcc cgtcccgtcg gcgacttcgg caactgctcg ctcggcagcg cctcccccga
     2701 ccgattctgg tgcccgacca ctcgcgtcgt cgcggcgtcg ggaccacccg actacatcgg
     2761 cgtgtacatc gcgacccggt atcaggcggt gacgaagatc ttcggcaagt cctacaacct
     2821 gtccgaacag accgtgtacc gcatggaagc gcagacgcga tgatgcggcg acgccgacgg
     2881 agcgaacgcg gggcgaactt cgtcgagatg gccctcgtgg tcccactgct cctgacgctg
     2941 atgctcggga tcttcgaggt cggcatgatg ttccgcagcg acatcgcgat cgccaacgcg
     3001 acccggaccg cggcgcgaac cggcagcacc gccggcaagg cacccaccgc cgacttccag
     3061 atcctcagct cgctcagcgc agcactcggc agcgtgaaga acgccaccgt gaactacgtc
     3121 gtcatctaca aggtgacggg gtcgaccaac gccgttcccg cagcctgcac cagcgcggcc
     3181 gccatcgcag cccacggcaa cgccgccaac ttctgcaaca cctacagcgc cgccgacctc
     3241 tcggcgatcc tggcgaaccc cgtgtccgca cagaccagct acggcggcag ctgcgccggc
     3301 gccggcaagg acaccaagtg gtgcccgacc acccgcgtgg tggaccaggg tgcgaccaac
     3361 gggcccgact acctcg
//
LOCUS       DAOSOA010000351         3376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_28220_length_3376_cov_4.980126, whole genome shotgun sequence.
ACCESSION   DAOSOA010000351 DAOSOA010000000
VERSION     DAOSOA010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28220_length_3376_cov_4.980126"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..655
                     /locus_tag="PKY13_15540"
     CDS             59..655
                     /locus_tag="PKY13_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81405.1"
                     /translation="MSGKASDPRAKVPRQTAAEARAEAQAAAAAGRAARDAAIAERFG
                     EGVPGAGILSATKVTTALFVVFTVVVTIVDTRGLRIALALFDSALFLAGCGLFLLALY
                     DGAQRSREADLTMSGWWFLSGSAPPSVRATLLGAVAVQTIVGLVTAAIRIETTLAFGI
                     LVPTLGLAFCGLWGARHGLFPPRRFAQEQVAIPKRRKR"
     gene            666..1826
                     /locus_tag="PKY13_15545"
     CDS             666..1826
                     /locus_tag="PKY13_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOT81406.1"
                     /translation="MRTILFTGKGGVGKTTVAAATALQCAQAGLRTAIMSTDPAHSLA
                     DSFDVDLDGELTEVAPKCWATQLDATERMETNWGEIRGYLRDVFDWAGLDEVEAEELS
                     IIPGLEEIFALTDITTFARSGDHDVLIVDCAPTAETIRFLSLPDVLSWYMDRVFPVGR
                     RLTQSLGPVVQRLAHVPVAGDQVFGAAERMYQQLDGVRELLRDGERSTVRLVVNPEKM
                     VIAEARRTYTYLSLFGYRVDAVVANRLLPDHIGDPWFSQWKASQVRHLESIDEGFAPL
                     PVLRAELADAEVVGIDRLDEFGASLYGDRRADDVLVDSDPLRIERHGDHMVMSLDLPF
                     ATKQELEVTRSPDELFVRVGPYRRAVLLPDSLRRREVAGASLLDGVLDVTFQ"
     gene            1870..2181
                     /locus_tag="PKY13_15550"
     CDS             1870..2181
                     /locus_tag="PKY13_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81407.1"
                     /translation="MTNGDDSAGPTPRLEDGVEHLQVAAHEMLAAARSFLDVVEDVVS
                     DREKLSEVTASVTELLGSAGGSLARMAERVAGSAGGASNPANDVDGGAPRPRVRRIDV
                     D"
     gene            2449..>3376
                     /locus_tag="PKY13_15555"
     CDS             2449..>3376
                     /locus_tag="PKY13_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81408.1"
                     /translation="MYLDLNRDSLELLELNQDLDVILRASDLPGSEHAMDESLHDDSA
                     ADPLGAAAVEDDEIREARRLAEDHDEAYESAVRSVEMLRHQLVEIESEQAVLNRQLGD
                     ARVGLDSFAAAGLKVAREELAELTTLVGASARATAPSNPASGATAEPSPASPIYDAAT
                     LRAERDRIESRLSVLIEQSDHLRRTIKQWRAIDPTPVREAYTALAGRTTPPGAAEAPG
                     SVDAPEAGEPVASTWEPSPEAQALADEWEAIDARLATIDPPRDQGRERLDALTSRRDA
                     TYDAMKLAERNLRQPELDPAAVDRLESIHDEIF"
BASE COUNT          478 a         1216 c         1174 g          508 t
ORIGIN      
        1 tcgccgcccg cctgggcgac gcgctcgggt gcgcgtcggt ggttcccgag gtggcagcgt
       61 gagcggcaag gcctccgacc cgagggcgaa ggttccccgc cagaccgctg cggaggcgcg
      121 cgccgaggcc caagccgcgg ccgcagcggg cagagcggcg cgcgacgctg ccatcgcgga
      181 gcggttcggc gagggagtgc ccggcgctgg catcctgtcg gcgaccaagg tcacgaccgc
      241 cctgttcgtg gtgttcacgg tggtcgtgac gatcgtcgac acgcgagggc tgcgaatcgc
      301 ccttgccctg ttcgactcgg cactgttcct cgccgggtgc ggcctcttcc tcctggccct
      361 ctacgacggc gcgcagcgca gtcgcgaggc cgacctgacg atgtcgggat ggtggttcct
      421 gagcgggtcg gctccgccgt cggtgcgggc cacactgctc ggcgcggtgg ccgtccagac
      481 gatcgtcggc ctggtcacgg cggcgatccg catcgagacg acgctggcgt tcggcatcct
      541 cgtccccacg ctcggcctcg cgttctgcgg cctgtggggc gcccgccacg ggttgttccc
      601 accccggagg ttcgcccagg agcaggtcgc gatcccgaaa cggcgcaagc ggtgaggccc
      661 gcacgatgag gacgatcctg ttcaccggca agggcggtgt cggcaagacg acggtcgctg
      721 cggcgaccgc gctccaatgc gcccaggcgg gtctgcgcac ggcgatcatg tccaccgacc
      781 cggcgcactc gctcgccgac agcttcgacg tcgacctcga cggcgagctc accgaggtgg
      841 cccccaagtg ctgggcgacc cagctcgacg ccaccgagcg tatggagacc aactgggggg
      901 agatccgcgg ctacctccgc gacgtcttcg actgggccgg cctcgacgag gtggaggccg
      961 aggagctgtc gatcattccc gggctcgagg agatcttcgc gctgaccgac atcacgacgt
     1021 tcgcccgctc aggcgaccac gacgtgctga tcgtcgactg tgcgcccacc gccgagacga
     1081 tccgcttcct gtcgttgccc gacgtcctca gctggtacat ggaccgggtg ttcccggtcg
     1141 ggcggcggtt gacccagtcg ctcggtccgg tggtgcagcg gctggcccac gttcccgtcg
     1201 ccggcgacca ggtcttcggc gcggcggagc ggatgtacca gcagctcgac ggggtccggg
     1261 agctgttgcg cgacggcgag cggtcaacgg ttcggctcgt ggtcaacccc gagaagatgg
     1321 tcatcgccga ggcccgccgc acctacacct acctctcgct gttcggctac cgcgtcgacg
     1381 cggtcgtcgc gaaccggctc cttcccgacc acatcggcga cccgtggttc agccagtgga
     1441 aggcatcgca ggtgcgccac ctcgagtcga tcgacgaggg cttcgctccg ttgcccgtgt
     1501 tgcgcgccga gttggccgac gccgaggtgg tgggcatcga ccgcctcgac gagttcgggg
     1561 cgtccctgta cggcgaccgc cgcgccgacg acgtcctcgt cgacagcgat ccgctgcgga
     1621 tcgaacggca cggtgatcac atggtcatgt cgctcgatct gcccttcgcg acgaagcagg
     1681 aactcgaagt gactcggagc cccgacgagc tgttcgttcg ggtcggcccg taccgtcgag
     1741 cggtgctgct gcccgattcg ctccgtcgcc gcgaggttgc cggcgcgagc ctcctcgacg
     1801 gcgtcctcga cgtcaccttc cagtgagccc acggcgtcga gcgaccgcgc cggctggcac
     1861 aatggcggca tgacgaacgg cgacgacagc gcggggccca ccccccgact cgaggacggc
     1921 gtcgaacacc tccaggtcgc cgcccacgag atgctcgcag cggcgcggag cttcctcgac
     1981 gtggtggaag atgtggtgtc ggaccgcgag aagctcagcg aggtgaccgc ctcggtcacc
     2041 gagctgttgg gcagcgccgg cggttcgctg gctcgcatgg ccgaacgtgt cgccggctcg
     2101 gccggcggtg cgtcgaaccc ggcgaacgac gtcgacggtg gggcgccgcg gccgcgggtt
     2161 cgccgcatcg acgtggactg atcctcttgg cgacggtcgt gggcgagttc acctccccaa
     2221 gttcgtgcag gttctcctca cgttgcgcgg tccgccgccg ataggacact tggtatgagg
     2281 ttggtacgct tcgaagacga atcaggcggc ttcgctgtcg acctgcaccc gctgatcacg
     2341 gtgatcagcg gccttcctgc gcacgtgcgc gatcgcctcg ttcacggcct agcggcgctc
     2401 ccgaggggca ccgatcccgg tggccgtggc tcgatcgagg ttcacggcgt gtatctcgac
     2461 ctgaaccgcg acagcctcga gttgctcgag ctcaaccagg acctcgatgt catcctgcga
     2521 gcgagcgacc tcccggggtc cgaacacgcc atggacgagt cgctccacga tgacagcgca
     2581 gccgaccccc tcggcgccgc cgccgtggag gacgacgaga tccgcgaggc ccgccggctc
     2641 gccgaggacc acgacgaggc ctacgaatcg gcggtgcgtt cggtcgagat gctccggcac
     2701 cagctcgtcg agatcgagtc ggaacaggcg gtgctcaacc ggcagctggg cgacgcccgt
     2761 gtcggcctgg actccttcgc cgccgccggt ctcaaggtcg ctcgtgagga gctcgccgag
     2821 ctgacgaccc tggtcggagc ctcggcccgc gccacggcgc cgtcgaaccc ggccagcggc
     2881 gcgacggccg aaccgagtcc ggcgagtccg atctacgacg ccgcgacgct ccgcgccgag
     2941 cgggatcgca tcgagagccg gttgtcggtc ctcatcgagc agtccgacca cctccgccgc
     3001 accatcaagc agtggcgcgc catcgatccg acgccggtcc gtgaggcgta caccgcgctc
     3061 gccggccgca ccacgccgcc gggcgctgcc gaggcccccg ggtctgtcga cgcccccgag
     3121 gccggcgagc ccgtcgcgtc gacctgggag ccgtcgcccg aggcccaggc gcttgccgac
     3181 gagtgggagg ccatcgacgc ccgcctcgcc accatcgacc cgcctcgcga ccagggtcgg
     3241 gagcggctcg acgcgttgac gtcgcgccgc gacgctacct acgacgccat gaagttggcc
     3301 gagcgcaacc tccgccagcc tgagctcgac ccggcggcgg tcgaccgcct cgagtcgatc
     3361 cacgacgaga tcttcg
//
LOCUS       DAOSOA010000352         3355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_28439_length_3355_cov_7.507576, whole genome shotgun sequence.
ACCESSION   DAOSOA010000352 DAOSOA010000000
VERSION     DAOSOA010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28439_length_3355_cov_7.507576"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKY13_15560"
     CDS             <1..185
                     /locus_tag="PKY13_15560"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOT81409.1"
                     /translation="RAASGPPELVPVIKQTIADMANVAEHGDAVERELDPQVESLNRP
                     AFAERLAAIQARITKK"
     gene            250..1494
                     /locus_tag="PKY13_15565"
     CDS             250..1494
                     /locus_tag="PKY13_15565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT81410.1"
                     /translation="MPSTDHRSLCIAEAYPWPAVDGYKLRLANMIEGLLLRGPVDFLC
                     LDGSGRHRDPAPDGVTVIDAPEAPEFPATVWMPRWLRSPDPRRLVRRDFSEARRVIST
                     LDQSRYDVTFFSHVDSWHQTHDLIRTPAFLDFDNLENLLTAGIRRMGPVIGPGAGAAV
                     RAAATARWLVASSFNLVDERRWDRVQRRAAATVGNVMVCSEVDIARSGCTNAVEVPNG
                     YQRVWDPVDHVTPNDPDHPVFLFIGLMGYEPNVDAVRWFASDVFPAIRRELPHAEFRI
                     VGRHTESVESLGELPGVTVAGSVESLQPELERADVSVVPLRSGAGTRLKVVEAMANRL
                     PVVSTAIGCEGIDVSDGVHLLVAEDAAAFARSCIRAVSDASLRADLIAAAEARYLETY
                     QWSTIRERVAALADQVSERRRS"
     gene            complement(1467..2573)
                     /locus_tag="PKY13_15570"
     CDS             complement(1467..2573)
                     /locus_tag="PKY13_15570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT81411.1"
                     /translation="MPAPQLPGGPHQTGRRVLVTGLGTFWGGRIAQALEADPTVDVII
                     GLDTTEPTVELERTEYVRVDANYSILAKIVRATRVDTIIHTFLVVDPTQMSKRAMHEV
                     NVIGTMNLFAAASAPDSTVRNVIVKSATAVYGCGPEDPTWFTEDTPPSRALRRPVERS
                     LDQVEGYVRDFAEDNPHVVVSLLRFSNVIGSEIVTPLTRALELPLVPSMFGFDPRFQF
                     VHEDDVVRSILFVLDHNLSGVYNVAGDGLLPWTEVASLCGKSTFPLPPYISDALLWPV
                     KRLGIVDLPEEYLNLLRFGRGVDNRKLKDAGFEYDYTSAQSVKAFIEAVRLRKTIGNN
                     TPSYSYERDVEQFFRHSPSVVREVPAYDRRRSDT"
     gene            complement(2573..>3355)
                     /locus_tag="PKY13_15575"
     CDS             complement(2573..>3355)
                     /locus_tag="PKY13_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT81412.1"
                     /translation="RHGDVDEWGRSERMRALARQVYDPLYSKWFRVEWEGLEKIPANG
                     GALLVANHAGAIPSDAPVIMHGIEKELERPVYGLADYFFRKVPVLGTMWSRLGGLPAH
                     PDNAYRLLREQKQLVLVFPEGTKGPSKHVSERYQLARFGRGGFVEIAMRAGVPVIPIA
                     VVGAEESMPIIAKLPSVAKAAGLPYMPVTANMMALGPLGLVGYFPAKFKLRVLDPVYF
                     NVEPDQERYSKSRIMDESEKIRDHIQTELYDMLRERRSIWFG"
BASE COUNT          519 a         1119 c         1172 g          545 t
ORIGIN      
        1 cgcgcgctgc ttcggggccg cccgagctgg tgccggtcat caagcagacg atcgcggaca
       61 tggcgaacgt tgccgagcac ggcgacgcgg tcgagcgcga gctcgacccg caggtggagt
      121 cgctcaaccg cccggcgttc gccgagcggc tcgctgcgat ccaggcccgc atcacgaaga
      181 agtgatcgct cgcccgacct acctggtgtg aacgcctgag ctgcggcgac cggccgctac
      241 cctgacccgg tgccatccac ggaccaccgg tcgctctgca tcgccgaggc ctacccttgg
      301 ccggccgtcg acggctacaa gttgcgcctc gccaacatga tcgaggggct gttgctccgg
      361 ggaccggtcg acttcctgtg cctcgacggc tcgggccgcc accgcgatcc ggctccggac
      421 ggcgtgacgg tgatcgatgc accggaggcg ccggagttcc cggccacggt ctggatgccc
      481 cggtggcttc gcagtcccga tcctcggcgt ctcgtccggc gcgacttctc ggaggcgcgg
      541 cgcgtcatct cgacgctcga ccagtcccgc tacgacgtga cgttcttctc ccacgtcgac
      601 tcgtggcacc agacccacga cctgatccgg acgccggcgt tcctcgactt cgacaacctc
      661 gagaacctgc tcaccgcagg tatccggcgg atgggtcccg tcatcggtcc cggtgccggc
      721 gcagcggtga gagcggcggc gacggctcga tggctggttg ccagctcgtt caacctggtc
      781 gacgagcgac gctgggaccg ggtgcagcgt cgggccgccg cgacggtcgg caacgtcatg
      841 gtgtgcagcg aggtcgacat cgcccgatcc ggatgcacca acgccgtgga ggtgcccaac
      901 gggtaccagc gggtgtggga cccggtcgac catgtcacac cgaacgatcc cgaccacccc
      961 gtcttcctgt tcatcggcct catggggtac gagccgaatg tcgacgcggt gcgatggttc
     1021 gcgtccgacg tgttcccggc gattcgtcgt gagctgcccc acgccgagtt ccgcatcgtc
     1081 ggccgccaca ccgagtcggt cgagtcgctc ggcgaactgc cgggcgtgac cgtcgccggc
     1141 tccgtcgagt cgttgcagcc cgagctcgaa cgtgccgacg tctcggtcgt gccgctgcgt
     1201 tcgggagccg ggacgcgcct caaggtggtc gaggcgatgg cgaaccggct cccggtcgtg
     1261 tcgacggcga tcggctgcga aggcatcgac gtgagcgacg gtgtgcacct cctcgtcgcc
     1321 gaggacgccg ctgcgttcgc ccgaagctgc atccgggcgg tgagcgacgc gtcgttgcgc
     1381 gccgacctga tcgcagcggc cgaggcccgg tacctcgaga cctaccagtg gtcgacgatc
     1441 cgtgagcggg tcgcggcgct ggccgatcag gtgtcggaac gccgccggtc gtaggccggc
     1501 acttcacgca cgaccgacgg cgagtgccgg aagaactgct cgacgtcgcg ctcgtaggag
     1561 tagctcggcg tgttgttgcc gatcgtcttg cgcaaccgca cggcctcgat gaacgccttc
     1621 accgactgcg ccgacgtgta gtcgtactcg aatcccgcgt ccttcaactt gcggttgtcg
     1681 acaccacgtc cgaagcgcag caggttgaga tactcctcgg gcaggtcgac gatgccgagt
     1741 cgcttcaccg gccagagcag cgcgtcggag atgtacggcg gaagtgggaa cgtggacttg
     1801 ccgcacagcg acgccacctc ggtccacggg aggaggccgt cgcctgcgac gttgtagacg
     1861 cccgagaggt tgtggtcgag gacgaacagg atcgaccgca cgacgtcgtc ctcgtgcacg
     1921 aactggaagc gagggtcgaa cccgaacatc gacggcacga gcggcagttc gagtgcccgc
     1981 gtcagcggcg tgacgatctc gctgccgatc acgttggaga agcgcaggag cgacaccacg
     2041 acgtgggggt tgtcctcggc gaagtcccgc acgtacccct cgacctggtc gaggctgcgc
     2101 tcgacggggc ggcgcaacgc ccgactcggc ggggtgtcct cggtgaacca cgtcgggtcc
     2161 tcggggccac agccgtacac cgcggttgcc gacttcacga tcacgttgcg gaccgtcgag
     2221 tcgggcgcgg aggccgcagc gaagaggttc atcgtgccga tgacgttgac ctcgtgcatg
     2281 gcccgcttcg acatctgcgt cgggtcgacg acgaggaacg tgtggatgat cgtgtcgaca
     2341 cgggtggccc gaacgatctt ggcgaggatc gagtagttgg cgtcgacccg cacgtactcg
     2401 gtgcgctcga gctccacggt gggctcggtc gtatcgaggc cgatgatgac gtcgacggtc
     2461 gggtcggcct cgagcgcctg ggcgattcgt ccgccccaga acgtaccgag gccggtgacg
     2521 aggacgcggc ggcccgtctg gtgaggtcct ccgggaagct ggggcgccgg catcagccga
     2581 accagatcga gcggcgctca cggagcatgt cgtacagctc cgtctggatg tggtcccgga
     2641 tcttctcgga ctcgtccatg atgcggctct tggagtagcg ctcctggtcg ggctcgacgt
     2701 tgaagtacac cgggtcgagg acccgcagct tgaacttggc cgggaagtag ccgacgagtc
     2761 cgagcggccc gagggccatc atgttggcgg tcacgggcat gtacggaaga ccagccgcct
     2821 tggcgaccga cgggagcttg gcgatgatcg gcatcgactc ctcggccccc acgaccgcga
     2881 tgggaatgac cggcacgccg gctcgcatgg cgatctcgac gaagccgccg cggccgaaac
     2941 gtgccaactg gtagcgctcg ctgacgtgct tcgacggccc cttggtgccc tcggggaaga
     3001 cgaggacgag ctgcttctgc tctcgcagca ggcggtaggc gttgtcggga tgcgctggga
     3061 ggccaccgag tcgcgaccac atcgtgccca gcaccggcac cttgcggaag aagtagtcgg
     3121 cgaggccgta caccggccgc tcgagctcct tctcgatgcc gtgcatgatc acgggggcgt
     3181 cggacgggat cgctcccgcg tggttggcga ccaggagggc accgccgttg gcggggatct
     3241 tctcgagacc ctcccattcg acccggaacc acttgctgta gagcgggtcg tacacctggc
     3301 gggccagcgc ccgcatgcgt tcggagcggc cccactcgtc gacgtcgccg tggcg
//
LOCUS       DAOSOA010000353         3342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_28588_length_3342_cov_4.868573, whole genome shotgun sequence.
ACCESSION   DAOSOA010000353 DAOSOA010000000
VERSION     DAOSOA010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28588_length_3342_cov_4.868573"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PKY13_15580"
     CDS             <1..1158
                     /locus_tag="PKY13_15580"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOT81413.1"
                     /translation="NLTRSFPPATQHNLFGWLSTLYPIWANIDEEASYLAAWIGFAEL
                     ALGGCTTTMDHMYVHPTGAGDLITAEITAAKELGMRFHATRGSMSRSKKDGGLPPDEV
                     VQDNDTILAESERLVGAHHDPTSHAMVQVALAPCSPFSVTPDLMKATAALAERLDVRL
                     HTHLAEDVDEDTYCLDVYGCRPLEYFEDVGWGSDRSWVAHCVYPNAAEIAKMAEWGTG
                     VAHCPSSNQMIGAGLAPIREYRDAGVPVGLGCDGSSSTDSASLWMETRNALLLGRLRG
                     GPEAMQAREALEMATLGGAQCLGREGVLGLLAPGTAADIVVWGLEGVQFAGAWSDPVE
                     ALLRCGPNAAKYTIIAGKVVVDDGRLVSPDVEDRLTRHEAVSRRLQGLALS"
     assembly_gap    1209..1308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1309..1865
                     /locus_tag="PKY13_15585"
     CDS             <1309..1865
                     /locus_tag="PKY13_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81414.1"
                     /translation="VLNNQSNFAKTYVRDQLSEQRISFTPVAGLSDEEKRADCLVENA
                     GKPLTTGKQAECYANEYIGLHVTEINGGKTYSESSGDARALQEQADAATKSDPDAAAT
                     TKLVTEAKIASGKVDALFKGETLRGLLLTTYGFTIFGERAAQAALVCFLVAAVLALAT
                     LAGLVHARRTPEGELVLPEVATGA"
     gene            1990..3255
                     /locus_tag="PKY13_15590"
     CDS             1990..3255
                     /locus_tag="PKY13_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT81415.1"
                     /translation="MTDIATPTTTDPRGLIFIDPAAFADMDQWHATAAELRAESPVLR
                     VEAPGWPAFWAVTKHADVFEVSRRSDVFFNTEKSAPGPDLQYDMLQALGIELPRTLVH
                     LHGDVHQKYRQVTNDWFKPAAVKGLQPAIDEIADEFVEKMAQMGGRCDFAADIAVPFT
                     LRVIMSIFGVPREDEALMLQLTQGLFGAADPEYLGDFSDPLALITDTIHTFESYFDEL
                     TAARRAHPTDDLATVIANGQVDGAPLGEIERLWYYIIVATAGHDTTSFSSSGGLEQLL
                     RHPDQLAAWQADPSLAVNATEEIIRWTSPVRSFFRWAQQDHELGGVTIPAGDIVLTAY
                     PSANRDEDVFADPMRFDITRPDADKLLSFGLGMHYCLGAQVARREVRTLLQKVLDRVA
                     TIEFDGEPSWTGAHFVSGVKHLPVSYTLR"
BASE COUNT          525 a         1163 c         1067 g          487 t
ORIGIN      
        1 aacctcacgc gatcgtttcc gccggccacg cagcacaacc tgttcggctg gctctccacg
       61 ctctacccca tctgggcgaa catcgacgag gaggcgtcgt acctggcggc gtggatcggc
      121 ttcgccgagc tcgccctcgg cggatgtacc acgacgatgg atcacatgta cgtgcacccc
      181 accggcgccg gcgatctgat caccgccgag atcacggcgg cgaaggagct ggggatgcgg
      241 ttccacgcca cccgcggttc gatgagccgg tcgaagaagg acggcggcct cccacccgac
      301 gaggtcgtgc aggacaacga cacgatcctc gccgaatcgg agcggctggt cggagcgcac
      361 cacgacccca cctcccacgc catggtgcag gtggcgctgg caccctgttc ccccttctcg
      421 gtcacgcccg atctgatgaa ggcgaccgcc gcgttggccg aacgactcga cgtgcgcctg
      481 cacacccacc tcgccgagga cgtcgacgag gacacctact gcctcgacgt ctacggctgc
      541 cgaccgctcg agtacttcga ggacgtcggg tgggggagcg accgctcctg ggtcgcccac
      601 tgcgtgtacc ccaacgccgc cgagatcgcg aagatggccg agtgggggac gggtgtggcg
      661 cattgtccga gctcgaacca gatgatcggc gccgggctcg cccccatccg ggagtaccgc
      721 gacgccggcg tgcccgtcgg cctcgggtgc gacggttcgt cgtcgaccga ctcggcctcg
      781 ctctggatgg agacgcgcaa cgccctcttg ctggggcggc tccgcggcgg acccgaagcg
      841 atgcaggcgc gcgaggcgct cgagatggcg acgctcggtg gcgcacagtg cctcggccgc
      901 gaaggggtgc tcgggttgct cgcgcccggg acggccgccg acatcgtggt gtggggactc
      961 gagggtgtgc agttcgcggg tgcctggagc gatccggtcg aagccctgct ccggtgcggg
     1021 cccaacgccg ccaagtacac gatcatcgcg ggcaaggtcg tggtggacga cggccgtctc
     1081 gtcagccccg acgtcgagga ccggctgacc cgacacgaag ccgtgtcccg acgcctccag
     1141 gggctggcac tctcctgacc actggccccg tgaccttcgg gagccggatg atgaccttcg
     1201 gccctgctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg gtcctgaaca
     1321 accagtcgaa cttcgcgaag acctacgtcc gcgatcagct gagcgagcag cgcatcagct
     1381 tcacgccggt agccggtctc tccgacgagg agaagcgtgc cgactgcctc gtcgagaacg
     1441 ccggtaagcc gctcacgacc ggcaagcagg cggagtgcta cgccaacgag tacatcgggc
     1501 tgcacgtgac cgagatcaac ggcggcaaga cctactccga gagcagcggc gacgcccgcg
     1561 ccctccagga gcaggccgac gccgcgacga agtcggatcc ggacgcagcg gcgacgacga
     1621 agctcgtcac cgaggcgaag atcgcgagtg gcaaggtcga cgccctgttc aagggcgaga
     1681 cgctccgcgg cctcctgctc acgacctacg ggttcacgat cttcggtgaa cgcgccgccc
     1741 aggcagccct cgtctgcttc ctcgtcgccg cagtcctggc gctggccacg ctcgctggcc
     1801 tcgtccacgc tcgtcggacg cccgagggcg agctcgtgct ccccgaggtc gcgacgggcg
     1861 cctgaggtcg gaggaccggg tcccggcggt tcgcccgtcg ggaccttcgg ccctgcctcg
     1921 gcgcggctac ggtgctcgac actgatcgaa acgcgatcac cgatcgcatc cgccggaccc
     1981 gaggtgacca tgaccgacat cgcgacaccc acgacgaccg accctcgcgg cctgatcttc
     2041 atcgatcccg cggcgttcgc cgacatggac cagtggcacg cgactgcggc cgaactccgc
     2101 gccgagtcgc ctgtcctccg cgtcgaggcc ccgggatggc cggcgttctg ggcagtgacc
     2161 aagcacgccg acgtgttcga ggtgtcacgg cgcagcgacg tcttcttcaa caccgagaag
     2221 tcggcgccgg gccccgacct ccagtacgac atgctgcaag ctctcggtat cgagctgccg
     2281 cgaacgctcg tgcacctcca cggcgacgtc caccagaagt accgccaggt caccaacgac
     2341 tggttcaagc ccgcagcggt caagggcctg cagccggcca tcgacgagat cgcggacgag
     2401 ttcgtcgaga agatggcgca gatgggtggg cgatgcgact tcgccgccga catcgccgtg
     2461 cccttcacgc tccgggtgat catgagcatc ttcggtgttc cccgcgagga cgaggcgttg
     2521 atgctgcagc tcacgcaggg cctgttcggt gccgccgacc ccgagtacct gggcgacttc
     2581 tccgacccgc tcgcgctcat caccgacacg atccacacgt tcgagtcgta cttcgacgag
     2641 ctgaccgccg cccgccgcgc gcaccccacc gatgacctcg ccaccgtgat cgccaacggc
     2701 caggtcgacg gagcgcccct cggggagatc gagcggctct ggtactacat catcgtggcg
     2761 acggccggcc acgacaccac gtcgttctcg tcgtcgggag gtctcgaaca gctcctccgt
     2821 caccccgacc agctcgccgc ctggcaggcc gacccgtcgc tggccgtcaa cgcgaccgag
     2881 gagatcatcc gctggaccag cccggttcgc agcttcttcc gctgggcgca gcaggatcac
     2941 gagctcggtg gtgtgacgat ccccgccgga gacatcgtgc tgacggccta cccgtcggcg
     3001 aaccgcgacg aggacgtgtt cgccgacccg atgaggttcg acatcacgcg acccgacgcc
     3061 gacaagctgc tctcgttcgg cctcggcatg cactactgcc tcggtgcgca ggtcgcccgt
     3121 cgggaggtcc gcaccctgct gcagaaggtg ctcgaccggg tagcgacgat cgagttcgac
     3181 ggcgagccgt cgtggaccgg cgcccacttc gtgagcggtg tgaagcacct gccggtgagc
     3241 tacaccctca ggtaggggcg gcgatcgacc gcgctcacga cctcactgcg tcgattcggt
     3301 cgccgatgtg ggcggtggtc tccgccggtt ccatggcccg gc
//
LOCUS       DAOSOA010000354         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_28833_length_3318_cov_5.432731, whole genome shotgun sequence.
ACCESSION   DAOSOA010000354 DAOSOA010000000
VERSION     DAOSOA010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28833_length_3318_cov_5.432731"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PKY13_15595"
     CDS             <1..769
                     /locus_tag="PKY13_15595"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOT81416.1"
                     /translation="KVTPEAMVESAMQELAYFDEVGFDNVKISVKASNVPLMIEAYRQ
                     LADATQAPLHLGVTEAGPGSAGLLKATAGIATLLAEGIGDTIRYSLTADPVEEARAGR
                     QLLEALGLRERKNIDLIACPSCGRAEIDVIAVAEQAQAAFGDREIPLQVAVMGCVVNG
                     PGEARDADIGIAAGNKRGHLFVRGTNVAVVPEDEMVDTLIEWAEFIMEHGIERALERG
                     DLTAAKEAAERDRAELLAEQGDDANDAGARIELIRRR"
     gene            769..1659
                     /locus_tag="PKY13_15600"
     CDS             769..1659
                     /locus_tag="PKY13_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81417.1"
                     /translation="MRGRALAALLVAAVVVAGCSSDEESSPPADTSSGLTTVPPSIVP
                     LTTTTTIEGTPVSDAEIYAQVGLTVEQGACIAASGAEGDFESTPGPALTDAMVLAAET
                     GAMVAIPATLRTSTELELTILTALAGDCAPADRLAALAAVDGQALDDAALTDDLPVRL
                     FHRTRDGATPAELACIEAKFREAPARLSSLAANPRLVESQCAPAERLASWRTTALDRG
                     LGAAEATSTERACLASPSAAADQALLAAAVDAVGSGDTSSDPLAGATPSCSNGARLYE
                     LALNIVALGDDFGVEPLQPS"
     gene            1876..2421
                     /gene="rimP"
                     /locus_tag="PKY13_15605"
     CDS             1876..2421
                     /gene="rimP"
                     /locus_tag="PKY13_15605"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOT81418.1"
                     /translation="MARTEVLHAIADPIVSSLGATIYDLEFEGGALKLTVDRPGGIDL
                     ETVAEVTRQVSRQLDLDDPIPGKYTLEVTSPGLERNLRTSSHWATAVGETARVKLKPH
                     VEGERRVEGTVTAVDDDVVTVEVAGSPVRLRIDDVDRARTVFVWGGQEKPGGPKNGAK
                     KMPTTNPTTASVAATDESEIA"
     gene            2418..>3318
                     /gene="nusA"
                     /locus_tag="PKY13_15610"
     CDS             2418..>3318
                     /gene="nusA"
                     /locus_tag="PKY13_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT81419.1"
                     /translation="MNPEMMDALHTLASERGISADTLFSLLADAMESAYKKLPGARPY
                     AWVVINPDTGEFRVMSQELDEDLEPIGEEFDVTPSDFGRIGAQAAKQRIGQGIREAER
                     ELKYEEYSGREGDIVTGIIQQSDARYTLLDLGKAEALMPQSEQVPYDRPQPGQRVKAY
                     IVEVRRTAKGPQIVVSRTHPGLIKRLFELEVPEIADGIVEIKACAREPGQRTKIAVYS
                     NDSNVDPVGACVGARGGRVRMVVNELNGEKIDIVPFTEDLPEFVMKALAPAKVTEVRI
                     DESTGTAEVIVPDYQLSLAIGKEG"
BASE COUNT          551 a         1163 c         1137 g          467 t
ORIGIN      
        1 caaggtcacg cccgaggcga tggtcgaatc cgcgatgcag gagctcgcct acttcgacga
       61 ggtcgggttc gacaacgtga agatctccgt gaaggcatcg aacgtcccgc tgatgatcga
      121 ggcgtaccgc cagctggccg acgccaccca ggcgccgctg catctgggcg tcaccgaggc
      181 cgggcccggc tcggcgggcc tgctcaaggc aacggccggc atcgcgacgc tgctcgccga
      241 gggcatcggc gacaccattc gatattcgct cacggccgac ccggtcgagg aagcccgtgc
      301 cgggcgtcag ctgctcgagg ccctggggct gcgcgagcgc aagaacatcg acctgatcgc
      361 ctgccccagc tgcggccgcg ccgagatcga cgtcatcgcc gtcgccgagc aggcccaggc
      421 ggcgttcggc gaccgtgaga tcccgctgca ggtcgccgtc atgggctgcg tcgtcaacgg
      481 gccgggcgag gcccgcgacg ccgacatcgg tattgccgcc ggcaacaaac gcgggcacct
      541 gttcgtgcgc ggcaccaacg tggccgtggt gcccgaggac gagatggtcg acacgctcat
      601 cgagtgggcc gagttcatca tggagcacgg catcgagcgg gccctcgaac gtggcgacct
      661 caccgcagcg aaggaggcgg ccgaacgcga ccgtgccgag ctcctggccg agcagggcga
      721 cgacgccaac gacgccggcg cacgcatcga gctcatccgc cgacgatgat gagggggcgg
      781 gccctcgctg cgctcctggt cgctgccgtt gtggtggccg ggtgttcgtc cgacgaggag
      841 tcgtcaccac cggcggacac gtcgtcaggg ctcaccacgg tgccgcccag catcgtgccg
      901 ctgaccacga cgacgacgat cgagggcacc ccggtgtccg acgccgagat ctacgcccag
      961 gtcggcctga ccgtcgagca gggcgcctgc atcgcggcat cgggggcgga gggcgacttc
     1021 gagtcgacgc ccggcccggc gctcaccgac gcgatggtgc tggccgccga gacgggggcg
     1081 atggtcgcga tcccggcgac cttgcgaacg agcaccgagc tcgaactgac gatcctgacg
     1141 gcgctcgcag gcgactgtgc cccggccgac cgtctcgccg cgctggcggc ggtcgacggc
     1201 caggcgctcg acgacgcggc gctgaccgac gacctgccgg tgcgcctgtt ccaccggacc
     1261 cgggacggag cgacccccgc cgaactggcc tgcatcgagg cgaagttccg cgaggcgccg
     1321 gcgcgtctgt cgtccctggc ggccaatccc cgtctcgtgg agtcgcagtg cgccccggcg
     1381 gaacggctcg catcgtggcg gaccaccgcg ctcgaccgag ggctcggggc cgccgaggcc
     1441 acgagcaccg aacgggcctg cctggcctcg ccgagcgcgg ccgccgacca ggccctgctg
     1501 gcagcggccg tcgacgcggt ggggtccggc gacacgtcga gcgatccgct ggccggggcc
     1561 acgccgtcgt gctcgaacgg cgcccggttg tacgagctcg cgctgaacat cgtcgcgttg
     1621 ggcgacgact tcggggtcga gccgctgcaa ccatcctgac cgtcggggcg cccgcgccga
     1681 gcgatccacc cggtacggcg ttactgatct atactttccg ccgtagcgtt tcgtgtaccg
     1741 gtggtacctc cgaaggcgtg ggcagtgctc gacaacaaga gcgcccacgc ctttcgtatg
     1801 agtgccgcgc ctcacggaat cttcgatcag gacgtatccc gtcagtcccc gatccgtcgg
     1861 aaggtaggtg agcacatggc cagaaccgaa gtcctccatg cgatcgccga cccgatcgtg
     1921 tcgtcgctcg gtgcgaccat ctacgacctc gagttcgagg gcggcgcgct caagctcacg
     1981 gtcgatcggc ccggcggaat cgatctggag accgtcgccg aggtcactcg ccaggtgtcg
     2041 cgccagctcg acctcgacga tccgatcccc ggcaagtaca cgctcgaggt cacgagtccc
     2101 gggctcgagc gcaatctccg cacgtcgtcg cactgggcca ctgcggtcgg tgagacggcg
     2161 cgcgtgaagc tcaagcccca cgtcgagggc gaacgtcgtg tcgagggcac ggtgaccgct
     2221 gtcgacgacg acgtcgtcac cgtcgaggtc gcgggctcac cggttcgcct ccgtatcgac
     2281 gacgtggacc gcgcccgcac ggtgttcgtg tggggcggcc aggagaagcc cggcggcccc
     2341 aagaacggcg ccaagaagat gccaacaacc aatccgacca cggcctcagt ggccgccacc
     2401 gacgagagcg agatcgcatg aacccagaga tgatggacgc actgcatacc ctggcgtccg
     2461 agcgcggcat ctcggccgac acccttttct ccctcctcgc cgacgccatg gagtcggcgt
     2521 acaagaagct tcccggtgcg cggccgtacg cctgggtcgt gatcaatccc gacaccggtg
     2581 agttccgggt gatgtcccag gagctcgacg aggacctcga gcccatcggt gaagagttcg
     2641 acgtgacccc gagcgacttc ggtcgcatcg gcgcccaggc tgccaagcag cgcattggtc
     2701 agggcatccg cgaggccgaa cgcgagctga agtacgagga gtactcgggc cgcgagggtg
     2761 acatcgtcac gggcatcatc cagcagagcg acgcccgcta cacgctgctc gacctcggca
     2821 aggccgaggc cctcatgccg cagtccgagc aggtgcccta cgaccgtccc cagcccggcc
     2881 agcgggtcaa ggcgtacatc gtcgaggtgc gccgcacggc caagggcccc cagatcgtgg
     2941 tgtcgcgtac ccaccccggc ctgatcaagc ggctgttcga gctcgaagtt cccgagatcg
     3001 ccgacggcat cgtcgagatc aaggcatgtg cccgtgaacc cgggcagcgc acgaagatcg
     3061 ccgtgtactc gaacgactcc aacgtcgatc ccgtcggtgc ctgcgtgggc gcccgcggtg
     3121 gtcgtgtccg catggtcgtc aacgagctca acggcgagaa gatcgacatc gtccccttca
     3181 ccgaggacct gcccgagttc gtgatgaagg cgctggcgcc ggcaaaggtg accgaggtca
     3241 ggatcgacga gtcgaccggc acggccgagg tcatcgtgcc cgactaccag ctgtcgctgg
     3301 ccatcggcaa ggagggcc
//
LOCUS       DAOSOA010000355         3307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_28970_length_3307_cov_5.083333, whole genome shotgun sequence.
ACCESSION   DAOSOA010000355 DAOSOA010000000
VERSION     DAOSOA010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28970_length_3307_cov_5.083333"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKY13_15615"
     CDS             complement(<1..277)
                     /locus_tag="PKY13_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81420.1"
                     /translation="MSELRIDPVFDAEFMSLPTDGWFDWSGGRGVDAEDWGAHADLLV
                     DGHLRFTVGAYLVRGGPVGDRVVLIDAGVGPETNPDDLIPPGVLPDSL"
     gene            complement(296..1096)
                     /locus_tag="PKY13_15620"
     CDS             complement(296..1096)
                     /locus_tag="PKY13_15620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOT81421.1"
                     /translation="MTGEPDGIGSQPAEAAGSSASAPLRSPRVDKLHQLPPTERRVAM
                     RRLASAGREVIELMSSSSATADDLDSAAESLEAVAAMLRGHTGGADYEGVAEMANAGD
                     LLAERRRMVEEGDPEAWAQFDYSPFIGLANPMSPPMHMDYEGDRVVCHVTFGSAFEGP
                     PACVHGGYVAAAFDEVLGAAQSLSGEQGMTARLVTNYRSPTPLHQPLRIEAWVDRREG
                     RKIFVQGTMHAGERLTAEAEGLFIAFDPDKFVKLMEARAEMQARSRQA"
     gene            complement(1093..1887)
                     /locus_tag="PKY13_15625"
     CDS             complement(1093..1887)
                     /locus_tag="PKY13_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOT81422.1"
                     /translation="MLIATWNVNSLKARMPRVEEWLETVQPDVLCLQETKLSDAQFPA
                     MAFAGLGYETAHHGYGQWNGVAILSKVGIDDVVCGFDDDLDPDPDSRIIWATCGGVRV
                     VSVYVPNGRTLDDDHYQYKLAWLARLRTHLDRHHTPDELLVILGDWNIAPADIDVWDI
                     RKFADSTHVSQPERDALQHIKDWGLVDTFRERYPDPGLYSYWDYRGGDFHQKRGMRID
                     YLLSSRALAETNRSDLVDRNARKGTKPSDHAPVLGWFDVPLPGAGS"
     gene            1914..2936
                     /locus_tag="PKY13_15630"
     CDS             1914..2936
                     /locus_tag="PKY13_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOT81423.1"
                     /translation="MTSPRPALDITPAPRGYHSEADLEAALDAAAAVGERVTRRVIGH
                     SVEGRPLHALTIAAPGRVPDPARTHVVIQGGMHGNEVIGTEVALHLLGFLMEDGVSGS
                     VATLLDLADVTVLPCVNPDGRQATMRSVASTRRLPTPRRNANGVDLNRNWPRPDGVAD
                     HWLPISGTPNPRLPWYRGPEPLSEPESRAILSVLEDRPPVAMLDLHSSGEILIYPWTS
                     KAEPPPDEAGYWQMIDAFRARQERRFTAKQSREWYPIVGSLDDYAYDRFGTLLLTVEL
                     GRPAEAVRSEPRRWKGGFWWANPLEPDVHARNVETGCFDALVAGVRYRTEHPGAERPG
                     VAPANS"
     gene            2994..>3307
                     /locus_tag="PKY13_15635"
     CDS             2994..>3307
                     /locus_tag="PKY13_15635"
                     /inference="COORDINATES: protein motif:HMM:NF027404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81424.1"
                     /translation="MADEQISSGTLGPNTWLVDEMYEQYLANPHSVSVTWQEFFADYR
                     PPGPGPSGVTTNGVPPANGAAPAAPPTPAAAAPATPAAPAATEPPAVPGDPIRGVGKK
                     IV"
BASE COUNT          500 a         1143 c         1176 g          488 t
ORIGIN      
        1 cgagtgaatc cggcaggacg cccggtggga tgaggtcgtc ggggttcgtc tcgggtccga
       61 cgcccgcgtc gatgagcacc acccggtcgc cgaccgggcc gccacgcacc aggtaggcac
      121 cgacggtgaa ccggaggtgg ccatcgacga ggagatcggc gtgcgcgccc cagtcctcgg
      181 cgtcgacgcc gcgcccgccc gaccagtcga accagccatc ggtcgggagc gacatgaact
      241 cggcgtcgaa gacgggatcg atacgaagtt cggacacgga ccctcccccg cgacgtcagg
      301 cctggcggga gcgggcctgc atctcggccc gggcctccat cagcttgacg aacttgtccg
      361 ggtcgaacgc gatgaagagg ccttcggcct cggccgtgag tcgttcgccg gcgtgcatcg
      421 tgccctgcac gaagatcttg cgaccctcgc ggcggtcgac ccacgcctcg atccgcagtg
      481 gctggtgcaa cggcgtcggt gagcggtagt tggtgacgag tcgggcagtc atgccctgct
      541 cgcccgacag cgactgggcg gcgcccaaca cctcgtcgaa cgctgcggcg acgtagccgc
      601 cgtggacgca ggcgggtggc ccctcgaagg ccgagccgaa cgtcacgtgg cacacgaccc
      661 ggtcgccctc gtagtccatg tgcatcggcg gtgacatggg gttggcgagg ccgatgaagg
      721 ggctgtagtc gaactgcgcc cacgcttcgg ggtcgccctc ctcgaccatt cggcggcgtt
      781 ccgccagaag gtcgccggcg ttcgccatct cggcgacacc ttcgtagtcg gctccgccgg
      841 tgtgaccgcg cagcatggcg gcgaccgcct cgagcgactc ggccgctgag tcgagatcat
      901 cagcggtcgc cgagctcgaa ctcatgagtt cgatgacctc gcgcccggcg ctggcgaggc
      961 ggcgcatggc gacacgacgc tccgtgggcg gcagttggtg gagcttgtcg acgcgggggc
     1021 tccgaagcgg agcactggcg gagctcccgg cggcctcggc gggctgggac ccgatgccat
     1081 cgggctcgcc agtcatgacc cagctccggg gagcgggacg tcgaaccacc cgaggaccgg
     1141 ggcgtggtcg gagggcttgg tgcccttgcg ggcgttgcgg tcgacgaggt cgctgcggtt
     1201 ggtctcggcg agcgcccggc tcgacagcag atagtcgatg cgcatccccc gcttctggtg
     1261 gaagtctccc ccgcggtagt cccagtagct gtagaggccg ggatcgggat accgctcacg
     1321 gaacgtatcg acgagccccc agtccttgat gtgctggagg gcgtcgcgct cgggttggga
     1381 gacgtgggtg ctgtcggcga acttcctgat gtcccacacg tcgatgtcgg cgggagcgat
     1441 gttccagtcg ccgaggatga cgaggagctc gtcgggggtg tgatggcggt cgaggtgggt
     1501 ccgcaggcga gcgagccacg caagcttgta ctggtagtgg tcgtcgtcga gcgttcgccc
     1561 gttggggacg tagaccgaca cgacgcggac gccgccgcac gtcgcccaga tgatgcggga
     1621 gtcggggtcg gggtcgaggt cgtcgtcgaa cccacagacg acgtcatcga tgccgacctt
     1681 ggacaggatg gcgacgccgt tccactgccc gtatccgtgg tgggcggtct cgtacccgag
     1741 gccggcgaag gccatggccg ggaactgggc gtcgctcagc ttggtctcct gcaggcacag
     1801 gacgtcgggc tgcaccgttt cgagccactc ctccacccgc ggcatccgag ccttgaggga
     1861 gttcacattc cacgtcgcga tcagcatcgg accgaacact atcgtcggcc ggcgtgacct
     1921 ctccccgccc ggcgctcgac atcaccccgg cgcctcgggg gtatcactcc gaggccgacc
     1981 tcgaggctgc cctcgacgcc gcggccgccg tcggcgagcg cgtgacgcgt cgggtcatcg
     2041 gacattcggt cgagggaagg ccgctgcacg ccctgacgat cgccgctccg ggccgtgtgc
     2101 cggaccccgc tcggacccac gtggtgatcc agggcggcat gcacggcaat gaggtgatcg
     2161 gcaccgaggt cgccctccac ctgctcgggt tcctgatgga ggacggggtg agcgggtcgg
     2221 tcgccacgct cctcgacctc gccgacgtca cggtgctgcc gtgcgtcaac cccgacggca
     2281 ggcaggcgac gatgcgcagc gtggcgtcga cgcgacggct cccgacgccg cgccgcaatg
     2341 ccaacggcgt cgatctcaac cggaactggc ctcgccccga cggcgttgcc gaccactggc
     2401 tgcccatctc cggcacgccg aacccccgat tgccgtggta ccgggggccc gaaccgctga
     2461 gcgaaccgga gagccgagcg atcctgtcgg tgctcgagga ccgcccgccc gtggcgatgc
     2521 tcgacctcca ctcgagcggc gagatcctca tctacccctg gacgtcgaag gcggagccgc
     2581 cgcccgacga ggccggctac tggcagatga tcgacgcgtt ccgggcgcgc caggaacgtc
     2641 ggttcacggc gaagcagtcc cgggagtggt atccgatcgt cgggtccctc gacgactacg
     2701 cgtacgaccg attcgggaca ctgctgctga cggtcgagct gggccggccg gccgaagcgg
     2761 tgcggagcga gcctcgccgg tggaagggcg gattctggtg ggccaacccg ctcgaacccg
     2821 atgtccacgc ccgcaacgtc gagaccggct gcttcgacgc actcgtcgcc ggagttcgct
     2881 accggaccga gcacccaggt gccgaacgac cgggcgtcgc acccgcgaac agctgagaaa
     2941 ccgccgcgag cgggctccga tgccgggttc gaggacatta ggcttcacgc ttgatggctg
     3001 acgagcagat ttcctcgggc accttgggcc ccaacacgtg gctggtcgac gagatgtacg
     3061 agcagtacct cgcgaacccc cactcggtga gtgtgacctg gcaggagttc ttcgccgact
     3121 accgcccgcc cggccccggg ccgagcggtg tcacgaccaa cggcgtcccg cccgcgaacg
     3181 gcgccgcccc ggcggcaccg cccactccgg ccgctgccgc cccggcgacc cccgccgcgc
     3241 cggcggcgac agagccgccg gcggtgccgg gcgacccgat tcgcggcgtc ggcaagaaga
     3301 tcgtcga
//
LOCUS       DAOSOA010000356         3181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_30391_length_3181_cov_4.802943, whole genome shotgun sequence.
ACCESSION   DAOSOA010000356 DAOSOA010000000
VERSION     DAOSOA010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30391_length_3181_cov_4.802943"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKY13_15640"
     CDS             complement(<1..292)
                     /locus_tag="PKY13_15640"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOT81425.1"
                     /translation="MSGAGTSILSVDTVALGSTASTKQAAIAEVAALLAAAGNIDPAY
                     GRSMLGREKVANTFLGGGIAIPHGLLKDREMILRTGIAVVQVPAGVEWNPGEP"
     gene            complement(289..1050)
                     /locus_tag="PKY13_15645"
     CDS             complement(289..1050)
                     /locus_tag="PKY13_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOT81426.1"
                     /translation="MYGPERQRLIVEEARHVGRVEVAVLAARFDVTSETVRRDLTALE
                     RQGLLRRVHGGAIPVERLGFEPGLDARAAAQTDQKERIAKAALAELPTEGAILIDAGT
                     TTGRLAEMLPADRELTVITNSLPIAMSLSGRPAITLLMIGGRIRGRTLATVDAWAQQA
                     LQDTFVDLAFVGTNGISVRRGLTTPDQAEAATKSAMIAAARRTVLLADHTKLGVDHLA
                     RFGTLADIDTFITDADADPELVADIEASGPTVVQA"
     gene            1148..1516
                     /locus_tag="PKY13_15650"
     CDS             1148..1516
                     /locus_tag="PKY13_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOT81427.1"
                     /translation="MDEARVDAYLWAIRLFKTRSLATDACKGGHVRINGHPAKAATKV
                     KIGDRVEARAQGRAWIVEVSRLIDKRVGAAIATTCYVDHSPPPPPPEFVAPPSIRDRG
                     TGRPTKKDRRAMDRFRGSGA"
     gene            1560..>3181
                     /locus_tag="PKY13_15655"
     CDS             1560..>3181
                     /locus_tag="PKY13_15655"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT81428.1"
                     /translation="MNDMSPADEALPTDPEGFAALGLHESLLTALAGLGYEEPTPIQR
                     EAIPPIIEGRDLLGQAATGTGKTAAFALPILERLHRGTRQNEPVALVLVPTRELAMQV
                     SEAIHKYGRAFDARVVPIYGGQPIVRQLQALKRGVDIVVATPGRAIDHIKRGTLPLDL
                     IRLVVLDEADEMLDMGFAEDLEAILDATPADRQTVLFSATMPPRIGGLARRHLTDPVT
                     IRIERETTEPGEAPRVRQVAYVVPRNHKAAALGRILDVEAPEAAIVFCRTRIEVDQLT
                     ETLNGRGYRAEGLHGGMSQEQRDRVMARVRNTTADLLIATDVAARGLDIDHLTHVVNY
                     DVPAAPESYVHRIGRVGRAGREGIAITLAEPREHRLLKNIERVIKMQIPVEQVPTVAD
                     LRNRRMELTLSSLRETIMVDDLNQFRVIVDTLADEFDVVEVALAAIKLAHEATAGADD
                     DAEEIPSVSLKPEKKSWEKKATHPGATTRGPGDKRRGGKDVGGSDAGRPKLQGRAAEE
                     GFTRLFVGTGRKGGIRPQDLVGAICGETDLQGR"
BASE COUNT          486 a         1187 c         1058 g          450 t
ORIGIN      
        1 ccggttcgcc cgggttccac tcgactccgg cgggcacctg caccacggcg atgccggtcc
       61 gcaggatcat ctcccggtcc ttcaacagcc cgtgggggat ggcgatgcca ccaccgagga
      121 aggtgttcgc caccttctcg cggccgagca tcgagcgccc gtacgccggg tcgatgttcc
      181 cggcggcggc gagcagcgcc gcgacctcgg cgatggccgc ctgcttggtc gacgcggtcg
      241 aaccgagggc aacggtgtcg acggagagga tggacgtgcc ggcgccgctc atgcctgcac
      301 caccgtcggg ccggatgcct cgatgtcggc gacgagttcg ggatcggcgt cggcgtcggt
      361 gatgaacgtg tcgatgtcgg cgagggtgcc gaaccgggcg agatggtcga cgccgagctt
      421 cgtgtggtcg gccaggagga cggtccgtcg cgctgcggcg atcattgccg acttggtggc
      481 ggcctcggcc tggtcggggg tcgtgagtcc ccgccgtacc gagatcccgt tcgtcccgac
      541 gaatgcgagg tcgacgaacg tgtcctggag ggcctgttgc gcccaggcgt cgacggtggc
      601 cagcgtgcgc ccccggatcc ggccgccgat catcaggagc gtgatcgccg ggcggcccga
      661 cagcgacatg gcgatcggca gcgagttggt gatgaccgtg agctcccggt cggccgggag
      721 catctcggcg aggcggcccg tggtcgttcc cgcgtcgatg aggatcgctc cctcggtcgg
      781 cagctcggcg agcgccgcct tggcgatgcg ttccttctgg tcggtctgcg cggcagcgcg
      841 ggcgtcgagt cccggttcga accccagccg ttccaccggt atcgcgccgc cgtgcacacg
      901 ccggagcagt ccctgacgct ccagggccgt gaggtcacgg cgaaccgtct ccgacgtcac
      961 gtcgaatcga gctgccaaca cggcgacctc gacccgtccg acgtgtcggg cctcctcgac
     1021 gatgaggcgc tggcgctctg gtccgtacat ctggaacccc ccggttcaat gttggtgtct
     1081 gtggttctac gcccgttcgt gttggttgtc aaccggcaac cggcgtcgaa gcactaccct
     1141 ccggcccatg gacgaagccc gggtcgacgc gtacctgtgg gcgattcgcc tcttcaagac
     1201 ccgatccctg gcgaccgacg cctgcaaggg cggccacgtc cggatcaacg gccaccctgc
     1261 gaaggccgcg accaaggtca agatcggcga tcgcgtcgag gcgcgggccc agggtcgggc
     1321 atggatcgtc gaggtctcgc ggctgatcga caagcgggtc ggcgccgcga tcgcgaccac
     1381 ctgctacgtc gaccacagtc cgccgccccc gccgccggag ttcgtcgccc cgccgtcgat
     1441 tcgcgaccgg ggcaccggtc gcccgacgaa gaaggaccgc cgggcgatgg atcgattcag
     1501 gggatcgggc gcctgaccag tacccttgcc tctcacccca catcgatagc gcagtcccca
     1561 tgaacgacat gtcccccgcc gacgaggccc tccccaccga ccccgagggt ttcgccgccc
     1621 tcgggctcca cgagagcctg ctcaccgcac tcgccgggct cggctacgaa gagcccaccc
     1681 cgatccagcg cgaggcgatc ccgccgatca tcgagggccg cgacctcctc ggccaggccg
     1741 ccaccggcac cggcaagacg gcggccttcg cccttccgat cctcgagcgc ctccaccggg
     1801 gcacccggca gaacgagccc gtcgcgctcg tcctcgtccc cacccgcgag ttggccatgc
     1861 aggtctccga ggcgatccac aagtacggcc gcgccttcga cgcccgggtc gtcccgatct
     1921 acggcggcca gcccatcgtc cggcagctgc aggcgctcaa gcgtggtgtc gacatcgtcg
     1981 tcgccacgcc gggccgagcg atcgaccaca tcaagcgtgg cacgctgccg ctcgacctga
     2041 tccgcctcgt ggtcctcgac gaggccgacg agatgctcga catgggcttc gccgaagacc
     2101 tcgaggcgat cctcgacgcg acgcccgccg accgccagac cgtgctcttc tcggcgacca
     2161 tgccgccgcg catcggcggt ctcgcccggc gccacctcac cgatcccgtc accatccgca
     2221 tcgaacggga gacgaccgag cccggcgagg ccccccgggt ccgccaggtc gcctacgtcg
     2281 tgccgcgcaa ccacaaggcc gctgccctcg gtcgcatcct cgacgtcgag gcccccgagg
     2341 ccgccatcgt gttctgccgg acccgcatcg aggtcgacca gctgaccgag acgctcaacg
     2401 gccggggata ccgcgccgag ggcctgcacg gtggcatgag ccaggagcag cgcgaccggg
     2461 tcatggcccg cgtccgcaac acgaccgccg acctcctgat cgccaccgac gtcgccgccc
     2521 gcggcctcga catcgaccac ctcacccacg tcgtcaacta cgacgtcccg gcggcgcccg
     2581 agtcctacgt gcaccgcatc ggccgggtcg gccgcgccgg gcgcgagggc atcgccatca
     2641 cgttggccga accgcgtgag caccgactgc tcaagaacat cgagcgcgtc atcaagatgc
     2701 agatcccggt ggagcaggtc ccgacggtcg ccgacctgcg caaccgacgc atggagctca
     2761 cgctgtcgag cctccgcgag acgatcatgg tcgacgacct caaccagttc cgcgtgatcg
     2821 tcgacacgct ggccgacgag ttcgacgtcg tggaagtggc gctggccgcc atcaagctcg
     2881 cccacgaggc aactgccggc gccgacgacg acgccgagga gatcccctcg gtctcgctca
     2941 agcccgagaa gaagtcgtgg gagaagaagg cgacccatcc cggcgcgacc acccgcggcc
     3001 ccggcgacaa gcgtcgtggt ggcaaggatg tcggcggctc cgacgccggc cggcccaagc
     3061 tgcagggccg cgccgccgag gaaggcttca cccgactgtt cgtcggcacc gggcgcaagg
     3121 gcggcatccg tccccaggat ctcgtcggtg cgatctgcgg cgagaccgac ctccagggcc
     3181 g
//
LOCUS       DAOSOA010000357         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_30584_length_3167_cov_5.998072, whole genome shotgun sequence.
ACCESSION   DAOSOA010000357 DAOSOA010000000
VERSION     DAOSOA010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30584_length_3167_cov_5.998072"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="PKY13_15660"
     CDS             complement(<1..1553)
                     /locus_tag="PKY13_15660"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOT81429.1"
                     /translation="MTAATLHRYNLADVWEAVSDRVPERVAVVCDGAELSYARLEERA
                     NRLANALLAHGVRAGDHVGVYLVNGPEYFEALLAAFKIRAVPININYRYVAGELRYLF
                     EDSQIVALIAHRQFSGRVADVAPTVPAIATVLSVEDGSGEPLAAGQLDYESELAAASA
                     ERDFESSVGPRSGDDLYVLYTGGTTGMPKGVVWRQEDAFFACIGGGDPMRMSGPVDEP
                     HQVLDRIIDFPFVAFPLAPMMHAAAQWTSLSWLFCGGSVVMNPGSFDALATWRAIEAH
                     KVSTLIVVGDAMVRPLIDAWDAHGPFDTSSLFAVGSGGAPLTASLKDRLMEILPTTMV
                     TDGFGSSETGAQGSQRLQPGERSGGSTRFVPMGDTTVVLDDDLVPVVPGSGVVGKVAL
                     TGRIPLGYYNDPEKSAATFVESGGKRWVLTGDMATVDEEGTIVLLGRGSQVINTGGEK
                     VYPEEVEAALKSHPSVYDAVVAGVPDERFGQRVCAIVAPVAGETVTLDELSTHCRQSV
                     AGYKVPRELV"
     gene            complement(1550..2542)
                     /locus_tag="PKY13_15665"
     CDS             complement(1550..2542)
                     /locus_tag="PKY13_15665"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT81430.1"
                     /translation="MSDEPLIVLDDHRPEPESGPVPEGVKGLDMPLGRRVRLDGRGTT
                     FVREIAGPSPDAPTVVLLHGWFASGGLNWFNCFAPLSEHFNIVAPDLRGHGRGIRNHR
                     RFRLASCADDVAALCDELGIERAIFCGYSMGGPVAQLMWRRHPHLVGGLVFCATSSSF
                     IPALQQRMIFAGSMALMASTTRTTQMVTRLPRPVRHALPKGLRGTSRPSSIQVWAAQE
                     MRRHDMRMVLEAGTAIGTFSSRRWIGSVEVPTTVLITTKDRAVSPSAQLALALAIPGA
                     TIQRYDEGHTSPVLESFGTAITGACVSVSDAISQRRRKSDRTSAPSTSRKGPTS"
     gene            complement(2539..3111)
                     /locus_tag="PKY13_15670"
     CDS             complement(2539..3111)
                     /locus_tag="PKY13_15670"
                     /inference="COORDINATES: protein motif:HMM:NF036523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT81431.1"
                     /translation="MTLTKPQQDRHERMLNVTARLASEGGYDAVQMRAVAVEADVALG
                     TLYRYFPSKEHLLVSAMLRQIETLYDRLVVKPPQGTDAVGRVIDVLRRANSALQRQQQ
                     FTVAVVRALAAGDETVAPVVREVRDLMAGIILAAIDTDEPTEKEQLVTEILQEVWLSS
                     LVAWISGVEPTASVNRKLEAATRLLLDGQP"
BASE COUNT          503 a         1096 c         1107 g          461 t
ORIGIN      
        1 accagctcgc ggggcacctt gtagccggcg acactctgac gacagtgggt cgacagctcg
       61 tcgagcgtga ccgtctcacc ggccacgggg gccacgatgg cgcacacccg ctggccgaac
      121 cgctcgtcgg gcaccccggc gacgacggcg tcgtacaccg acggatgcga cttgagcgct
      181 gcctcgacct cctcggggta caccttctcg ccaccggtgt tgatgacctg ggacccccga
      241 ccgaggagca cgatggtgcc ttcctcgtcg acggtcgcca tgtctccggt gagcacccac
      301 cgtttgccgc ccgactcgac gaacgtcgcg gccgacttct cgggatcgtt gtagtagccg
      361 agcgggatgc gaccggtgag cgcgaccttg ccgacgacac ccgatcccgg gaccaccgga
      421 acgaggtcgt cgtcgagcac cacggtggtg tcgcccatcg ggacgaagcg ggtgctgcca
      481 ccgctgcgtt cacccggctg gaggcgctgc gaaccctgcg ctcccgtctc ggaggacccg
      541 aacccgtcgg tgaccatcgt cgtgggcagg atctccatca ggcggtcctt caacgatgcc
      601 gtgagcggcg ctccgcccga cccgacggcg aacaggctcg atgtgtcgaa cggcccgtgc
      661 gcgtcccacg cgtcgatcag cgggcgcacc atcgcgtcgc ccacgacgat gagcgtcgag
      721 accttgtggg cctcgatcgc ccgccaggtg gcgagggcgt cgaacgatcc cgggttcatc
      781 acgaccgacc caccgcagaa cagccacgac agcgatgtcc actgcgccgc ggcgtgcatc
      841 atcggggcca gggggaacgc gacgaacggg aagtcgatga tgcggtcgag cacctgatgc
      901 ggttcgtcga cggggccgct catgcgcatc ggatccccac ctccgatgca ggcgaagaac
      961 gcatcctcct gacgccacac cactcccttg ggcatgcccg tggtgccacc ggtgtagagc
     1021 acgtagaggt cgtcgcccga acgcggtccg accgacgact cgaagtcacg ctccgccgaa
     1081 gctgccgcca gctccgactc gtagtcgagc tggccggcgg ccagcggctc acccgacccg
     1141 tcctcgacgg acagcaccgt tgcgatggcc ggcaccgtcg gcgccacgtc ggcgacacgc
     1201 ccggagaact gccggtgcgc gatgagcgcc acgatctggg agtcctcgaa gaggtaccgc
     1261 agctcaccgg cgacgtaccg gtagttgatg ttgatcggca ccgcccggat cttgaacgcc
     1321 gccaggaggg cctcgaagta ctcggggccg ttgacgaggt agaccccgac gtggtcgccg
     1381 gcgcgcacac cgtgcgccag cagcgcgttc gcgaggcggt tcgcccgctc ctcgagccgg
     1441 gcgtaggaca actcggcgcc gtcgcagacg accgcgaccc gttccgggac gcgatccgac
     1501 accgcctccc acacgtcggc gaggttgtac cggtggagcg tggcggcggt cacgaggtgg
     1561 gtcccttcct ggacgtactg ggagcggagg tgcgatcgga cttccggcgg cgctgagaga
     1621 tcgcgtcgga cacgctcacg caggcgccgg tgatggcggt accgaacgat tcgaggaccg
     1681 gcgaggtgtg cccctcgtcg tagcgctgga tcgtcgcgcc ggggatcgcc agcgccaacg
     1741 ccagctgggc cgacggcgag acggcacggt ccttggtcgt gatgagcacg gtggtcggca
     1801 cctcgacgct gccgatccag cgccgcgagg agaacgtacc gatcgcggtg cccgcctcga
     1861 gcaccatgcg catgtcgtgg cggcgcatct cctgcgcggc ccacacctgg atcgacgacg
     1921 gcctcgacgt gccgcggagg cccttgggca gcgcgtggcg caccggccgc gggagccgcg
     1981 tcaccatctg ggtcgtccgc gtcgtgctcg ccatgagcgc catcgatccc gcgaagatca
     2041 tgcgttgctg caaggcgggg atgaacgacg agctggtggc gcagaagacg agaccgccga
     2101 cgaggtgggg atggcggcgc cacatgagct gggcgaccgg cccacccatg gagtacccgc
     2161 agaagatggc acgctcgatg ccgagctcgt cgcacaacgc cgccacgtcg tcggcgcagg
     2221 acgcgaggcg gaaccgacgg tggttccgga tgccgcgccc gtgtccgcgg aggtcgggcg
     2281 cgacgatgtt gaagtgctcg ctgaggggag cgaagcagtt gaaccagttg agtccccccg
     2341 atgcgaacca cccgtggagg agcaccacgg tgggggcgtc gggggagggg ccggcgatct
     2401 cgcgcacgaa ggtggtaccg cggccgtcga gccggacgcg ccgaccgagc ggcatgtcga
     2461 gccccttgac cccttcgggg accgggccgc tctcgggttc gggtcggtgg tcgtcgagca
     2521 cgatcaacgg ttcatcgctc acggctggcc gtccaggagc agcctcgtcg cggcctcgag
     2581 cttgcggttg accgacgccg tgggttctac gccgctgatc cacgcgacga gcgacgacaa
     2641 ccagacctcc tgcaggatct cggtcaccag ttgctccttc tcggtcggct cgtcggtgtc
     2701 gatggcggcg aggatgatgc cggccatcag gtcgcgcacc tcgcgcacca ccggagcgac
     2761 cgtctcgtcg cccgcggcga gggcccggac gacggcgacg gtgaactgct gttggcgttg
     2821 cagggccgag ttggcacgcc ggagcacgtc gatgacccgc ccgaccgcat cggtgccctg
     2881 cgggggcttg accaccagcc ggtcgtagag cgtctcgatc tggcggagca tcgccgacac
     2941 cagcaggtgc tccttggagg ggaagtagcg gtagagggtg ccgagtgcca cgtcggcctc
     3001 gacggcgacc gcccgcatct gcacggcgtc gtacccacct tcactcgcca gccgagcggt
     3061 gacgttgagc atccgctcgt ggcgatcctg ctggggtttg gtgagtgtca cgaccgttcc
     3121 tcctcggccg aacccgccga ccgaccccct atcgtggcac gaaactg
//
LOCUS       DAOSOA010000358         3161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_30684_length_3161_cov_5.392788, whole genome shotgun sequence.
ACCESSION   DAOSOA010000358 DAOSOA010000000
VERSION     DAOSOA010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30684_length_3161_cov_5.392788"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PKY13_15675"
     CDS             <1..440
                     /locus_tag="PKY13_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81432.1"
                     /translation="ANPTHSFKDRVVAVAIATDGVETVPVTFAKSDTAGEYEATVSFP
                     APGAWKVRFDVAEPEGTLTVDHAVAASTTVAPTTGAPTTEAPTTTGAPTTEAPTTTAA
                     SDDDDGESSGGAAVVVAALASGVVLMSAIVMSRRRRDRGKASD"
     gene            440..1312
                     /locus_tag="PKY13_15680"
     CDS             440..1312
                     /locus_tag="PKY13_15680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT81433.1"
                     /translation="MTTPLTGTGIWSSGIRYGDRGQALDAAAEIEHLGYTAIWLPDIG
                     GDLFEVVEAVLRATSTITVATGILNLWMHEASETAERHHAFVEEFGHRVLFGIGISHA
                     PLIDATVEGGYSRPLATTRAYLDAIDAQPIPVPADERLLAALGPKMLELAGTRAGGTH
                     PYLSTPVHTARARAALGPDRLVAPEQGVVLETDPAEARRIARIHLSTYLALPNYSNNW
                     LRLGFTEDDLLDGGSDRLVDALVAWGDEAKILERVQEHRDAGADHVCVQVLGEDRVEF
                     PLDAWRALAPVLLG"
     gene            complement(1328..2146)
                     /locus_tag="PKY13_15685"
     CDS             complement(1328..2146)
                     /locus_tag="PKY13_15685"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HOT81434.1"
                     /translation="MSVIHPTVVDDAVFGAALAAGTANIIMQLSRPGVGHGVVESPVD
                     SGKATLHPIKRARTTFTYLAVALWGTDVERKVYRNAVNGSHAQVRSTDASPVEYNAFD
                     PDLQLWVAACLYRGLEDVHRAMFPDSNFRGEHGIYEASHTLGSTLQVKADRWPVDRDA
                     FERYWKEGLELVSIDDTVRRYLTDLTDMTFLPAPIRLALAPNHRFNTIGFLPEQFRDE
                     MHFSWSAAQQRRFDHTWRAIGLVNQVLPAPVRHLPYNVYLADFRRRVRAGTQLV"
     gene            2314..2586
                     /locus_tag="PKY13_15690"
     CDS             2314..2586
                     /locus_tag="PKY13_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81435.1"
                     /translation="MNGLVPPTRTELSFAPIYEQIMAMGAILRNADRPPVPGRAVGCG
                     RAKLALDAIDGPADGGRCQHVSVLRQRYETLAAVDPAATTHDLAEA"
     gene            complement(2614..>3161)
                     /locus_tag="PKY13_15695"
     CDS             complement(2614..>3161)
                     /locus_tag="PKY13_15695"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT81436.1"
                     /translation="RAQFESSVIGPARLSRLAVERLRAEGRPGRLINISSMLASTDLP
                     FTGWYSAAKAAFDVVGDALSLELRPSDIDVVRVECGAVRTGAWDIAGTDVLAGDDPTT
                     RRARRRWVDLTDRVKPWFASPAEVGAVVARAATDERPKAVYRVGFASRLGVVADLVPA
                     PVEHAVTARLFGLRRPGQRVY"
BASE COUNT          489 a         1109 c         1111 g          452 t
ORIGIN      
        1 ccgccaaccc gacccattcc ttcaaggacc gggtggtcgc cgtggcgatc gccaccgatg
       61 gcgtcgaaac ggtgccggtc acgttcgcga agtccgacac tgcgggcgag tacgaggcca
      121 ccgtgtcgtt cccggccccc ggtgcgtgga aggtccgttt cgacgtcgcc gaacccgagg
      181 gcacgctcac cgtcgaccat gccgtcgccg cgtccacgac ggtcgccccg accaccgggg
      241 cgccgacgac cgaagcgcca acgacaaccg gggcgccgac gaccgaagcg ccgacgacga
      301 cggccgcgtc cgacgacgac gatggcgagt cgtcgggcgg ggcagcggtc gtggtggccg
      361 cgctcgcctc gggtgtggtg ctgatgagcg cgatcgtgat gtcacgacgg cgacgagacc
      421 gagggaaggc atccgactga tgacaactcc actgacgggg accgggatct ggagcagcgg
      481 catccggtac ggcgatcggg gccaggcgct cgacgctgcg gccgagatcg aacacctcgg
      541 gtacaccgcc atctggctgc ccgacatcgg cggtgacctc ttcgaggtcg tcgaggcggt
      601 gctccgggcg acctcgacga tcacggtcgc caccggcatc ctcaacttgt ggatgcacga
      661 ggcgtccgag acggccgaac ggcaccacgc cttcgtcgag gagttcggcc atcgcgtcct
      721 gttcggcatc ggcatcagcc acgcgccgct catcgacgcg acggtcgagg gcggttactc
      781 ccgcccgctg gcgaccaccc gggcctatct cgacgccatc gacgcacagc cgatcccggt
      841 gcctgccgac gagcgcctgc tcgcagcgct cgggcccaag atgctcgagc tcgccggcac
      901 ccgagccggt ggcactcatc cgtatctctc gacaccggta cacaccgcgc gggcccgagc
      961 tgcactcggt cccgaccggc tcgttgcccc cgaacagggc gtggtgctcg agaccgaccc
     1021 tgccgaagcc cgtcgcatcg cccggatcca cctcagcacc tacctggcgc tcccgaacta
     1081 ctccaacaac tggctgcggc tcggcttcac cgaggacgac ctgctcgacg gggggagtga
     1141 ccggctcgtc gacgcgctgg tcgcctgggg tgacgaggcg aagatcctcg agcgggtgca
     1201 ggagcatcgc gacgccggcg ccgaccacgt gtgtgtgcag gtgctcggcg aggaccgggt
     1261 ggagttcccg ctcgacgcct ggcgggcgct ggctccggtc ctcctcggct gatcgtcccg
     1321 gctcgggtca gaccagctgc gtcccggcgc ggacccgccg gcggaagtcg gccaggtaca
     1381 cgttgtacgg caagtggcgc accggggcgg ggaggacctg gttcaccagg ccgatggcgc
     1441 gccacgtgtg gtcgaaccgc cgctgctgtg ccgccgacca cgagaagtgc atctcgtcgc
     1501 ggaactgctc ggggaggaac ccgatcgtgt tgaaccggtg attcggtgcg agcgcgaggc
     1561 ggatgggggc tgggaggaac gtcatgtcgg tcaggtcggt gaggtagcgg cggaccgtgt
     1621 cgtcgatcga caccaactcg aggccctcct tccagtacct ctcgaacgcg tcacggtcga
     1681 ccggccagcg gtcggccttg acctggagtg tgctgcccag ggtgtgtgac gcctcgtaga
     1741 taccgtgctc gccgcggaag ttcgagtcgg ggaacatggc gcggtgcacg tcttcgaggc
     1801 cccggtagag gcacgccgcg acccagagct ggaggtcggg gtcgaacgcg ttgtactcga
     1861 cggggctggc gtcggtcgaa cgaacctggg cgtgggaacc gttcactgcg ttgcggtaca
     1921 ccttgcgttc gacgtcggtg ccccacaacg cgacggcgag gtaggtgaac gtggtgcgcg
     1981 cccgcttgat cgggtgcagc gtggccttgc cggagtcgac gggactctcg acgacgccgt
     2041 gcccgacccc gggccgtgac agctgcatga tgatgttggc cgtaccggcg gcgagcgcgg
     2101 cgccgaacac ggcatcgtcg acgacggtgg ggtggatgac gctcatcgat cctccgtatt
     2161 ggcggttcgc caatagtacg cacgtcaggc tactcggcac ccgttggtgg tcgcctccgc
     2221 cgctcgggcg acaccgtcgt ccagccacct tcggggtagg cccagcggat cattcggcgt
     2281 tggcggccga ccatcgcggg tacgggagca tccatgaacg gcctcgtccc cccaacccgg
     2341 accgagctga gcttcgcccc catctacgag cagatcatgg cgatgggggc gatcctccgg
     2401 aacgccgacc gcccgccggt gccgggccgt gccgtcgggt gcggtcgagc gaagctggcg
     2461 ctcgacgcca tcgacgggcc cgcggacggg ggtcgctgcc aacacgtgag tgtgctccgg
     2521 caacggtacg agacactcgc cgcggtcgac cccgccgcca cgacccacga cctcgcggag
     2581 gcctgacggt cgacaccgtc tcaattcgcg cactcagtac acgcgctgcc caggacgccg
     2641 gaggccgaac agtcgcgccg tgacggcgtg ctccaccggc gccggtacca agtcggcgac
     2701 gacgcccagc cgggacgcga accccacccg gtacaccgcc ttcgggcgct cgtcggtggc
     2761 ggcgcgggcc accacggcgc ccacctccgc tggtgaggcg aaccacggtt tcacccgatc
     2821 ggtgagatcg acccatcggc gccgggcgcg gcgcgtggtc gggtcgtcgc cggccaggac
     2881 gtcggtgccc gcgatgtccc acgcccccgt tcgaaccgcg ccgcactcga cgcggacgac
     2941 gtcgatgtcg gacggtcgca actcgagcga caatgcgtcg ccgacgacgt cgaacgccgc
     3001 cttcgctgcg ctgtaccaac cggtgaacgg gaggtccgtc gaagcgagca tcgaggagat
     3061 gttgatcaga cgcccgggcc gcccctcggc ccggagccgc tcgaccgcga ggcggctgag
     3121 gcgtgccggt ccgatcaccg acgactcgaa ctgggcgcgg a
//
LOCUS       DAOSOA010000359         3158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_30718_length_3158_cov_6.293265, whole genome shotgun sequence.
ACCESSION   DAOSOA010000359 DAOSOA010000000
VERSION     DAOSOA010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30718_length_3158_cov_6.293265"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKY13_15700"
     CDS             complement(<1..268)
                     /locus_tag="PKY13_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT81437.1"
                     /translation="MEADRAAELLALDRAHVWHPYASMPGALDLTLIESAAGVRLRLA
                     QPIGDVTELIDGMSSWWAAIWGYRHPVLDAAAHAQIDTMSHVMFG"
     gene            complement(268..1299)
                     /gene="bioB"
                     /locus_tag="PKY13_15705"
     CDS             complement(268..1299)
                     /gene="bioB"
                     /locus_tag="PKY13_15705"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOT81438.1"
                     /translation="MTDTSTFSAETTTDDIIAAARTRLVDEGGWLTLDEVTALSKLPT
                     KRLGDLMSLAHEVRLARCGDTVEVEGILSVKTGGCPEDCHFCSQSAQFDTPVAATPFL
                     DTEEVLQAARETAELGATEFCIVLAVRGPDEKTLQRILELVPIIGDETGLDVAVSAGI
                     LDADQARRLAEGGVHRYNHNLETSRSYFPEVVKTHGFDERWETCELVKANDMELCCGV
                     LLGMGESDDQRLELIGHLQELRPQEVPVNFLNPRPGTPLELKPITPAKDALKWVALFR
                     LALPEVILRYAGGREITLGDMQEAGMTAGINALIVGNYLTTLGRSPDEDLDMLERLSM
                     PVGALSKVV"
     gene            1403..>1633
                     /locus_tag="PKY13_15710"
     CDS             1403..>1633
                     /locus_tag="PKY13_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81439.1"
                     /translation="MGTELGESGRRGESWSATAEVAGLGVRAEDRWREIRTLGANGVR
                     TSLPDGRQVVQFASNDYLGLSGHPSVIAASVAA"
     assembly_gap    1634..1733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1734..2462
                     /locus_tag="PKY13_15715"
     CDS             <1734..2462
                     /locus_tag="PKY13_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT81440.1"
                     /translation="RQARVPVSVYAHNDVEAARAAIVEQGAAGRRSVIVTDSVFSMDG
                     DVAPLFELAELALTTGALLVVDDAHAVFAGHGADALASSDAEILIVGTLSKALGSQGG
                     FVAGPRSLVDWCRNTARSFIFSTASPPSVVAAALAALAVVRSDEGRALTAALRANVDA
                     LLPGHLSAVVPVVLGEERRALDVSAALLDRGLLVPAIRPPTVAVGASRLRIAVSAAHT
                     PADVAELIEALGALGVTLDAEVAA"
     gene            2459..>3158
                     /locus_tag="PKY13_15720"
     CDS             2459..>3158
                     /locus_tag="PKY13_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOT81441.1"
                     /translation="MSERPRRLVVVAGTATEIGKTWVSAALATELVADHWAVEARKPA
                     QSGGPGDPSDADLLAAATEEASTTVCPPHRRYSVPMAPPMAAEATGLEPPTIAELVGE
                     VAASWGPRAADVGLVELAGGVASPAGADGDGADLAAALAPDAVVLVADAGLGTINAVR
                     LSLARLAVVGLNAVVYLNRYDDGDDLHRRNRAWLADRDGLSVLTSISALVEWTLGRAP
                     LHCGYCGKPVTECDS"
BASE COUNT          405 a         1011 c         1164 g          478 t
ORIGIN      
        1 caccgaacat gacgtggctc atcgtgtcga tctgagcgtg ggctgcggca tcgaggaccg
       61 ggtgccggta tccccagatc gccgcccacc acgagctcat gccgtcgatc agttcggtga
      121 cgtcgccgat cggttgagcc agtcggaggc ggacaccggc ggccgattcg atcagcgtca
      181 ggtcgagcgc gcccggcatg gacgcgtacg ggtgccacac gtgggcccgg tcgagcgcga
      241 ggagctcggc ggcccggtcg gcctccacta gacgaccttg gagagcgctc ccaccggcat
      301 cgacaggcgc tcgagcatgt cgaggtcctc gtcgggcgaa cggccgaggg tggtgaggta
      361 gttgccgacg atgagtgcgt tgatgccggc ggtcatgccg gcctcctgca tgtcgccgag
      421 cgtgatctcg cggccaccgg catagcggag gatgacctcc ggaagggcca ggcggaacaa
      481 cgccacccac ttgagggcgt ccttggccgg cgtgatgggc ttgagctcga ggggagtgcc
      541 ggggcgcggg ttcaggaagt tgacgggcac ctcctggggc cgcagctcct gcagatgacc
      601 gatgagctcg aggcgctggt cgtcggactc gcccatgccg aggaggacac cgcagcacag
      661 ctccatgtcg ttggccttga ccagttcgca ggtctcccac cgctcgtcga agccgtgggt
      721 cttgacgacc tcggggaagt agctgcggga ggtctcgagg ttgtggttgt aacggtggac
      781 cccgccctcg gcgaggcgcc gggcctggtc ggcgtcgaga atgccggccg agactgcgac
      841 gtcgaggccg gtctcgtcgc cgatgatcgg cacgagctcg aggatccgct ggagcgtctt
      901 ctcgtcgggg ccgcggacgg cgagcacgat gcagaactcg gtggcgccga gctcggccgt
      961 ttcgcgggcg gcttgtagca cctcctcggt gtcgaggaac ggcgtcgcgg cgacgggcgt
     1021 gtcgaactgc gcggactggc tgcagaagtg gcagtcctcg gggcagccgc cggtcttgac
     1081 cgacaagatg ccctcgactt cgacggtgtc cccgcatcgg gcgagccgga cctcgtgggc
     1141 cagcgacatg aggtcgccga ggcgcttggt cggcagcttc gacagcgccg tcacctcgtc
     1201 gagggtgagc cagccacctt cgtcgacgag tcgcgtgcgg gcggccgcga tgatgtcgtc
     1261 ggtggtggtc tcggcggaga aggtggaggt gtcggtcacg atgccctcgg tgctggagtc
     1321 gattcggccg cgtgcggccc ggtacgttcg gaggcggtcc cgccccggac gttcgtgggg
     1381 gagagggtac cgggaggcac gcatgggaac cgaactcggc gagagcggac ggcgcggcga
     1441 gagctggtcc gcgacggccg aggtcgccgg cttgggcgtc cgggccgagg accgctggcg
     1501 cgagatccgc accctcggtg ccaacggcgt ccgcacgtcg ttgcccgacg gtcggcaggt
     1561 cgtgcagttc gcctccaacg actatctcgg cctcagtggc catccgtcgg tgatcgccgc
     1621 gtcggtggcc gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtcagg
     1741 cccgcgtgcc cgtctcggtg tacgcacaca acgatgtcga ggccgcacgg gcggcgatcg
     1801 tggagcaggg tgcagccggt cgccggtcgg tgatcgtcac cgactcggtg ttctcgatgg
     1861 acggcgacgt cgcgcccttg ttcgaactcg ccgagctggc gctgaccacg ggtgcgctcc
     1921 tcgtcgtcga cgacgcccac gccgtgttcg cggggcacgg tgccgatgca ctggcctcgt
     1981 cggacgccga gatcctgatc gtgggcacgc tgtccaaggc gctgggaagc cagggcgggt
     2041 tcgtcgccgg gccacgatcg ctcgtcgact ggtgccgcaa caccgcacga tcgttcatct
     2101 tctcgacggc atcgccgccg tcggtcgtcg ccgcggccct cgccgcgttg gcggtcgtgc
     2161 gatcggacga ggggcgtgcg ctcaccgctg cgttgcgggc gaacgtcgac gcgctgttgc
     2221 cgggccacct gagcgccgtg gtcccggtcg tcctcggcga ggaacgccgt gcgctcgatg
     2281 tcagcgcagc gctgctcgat cgaggcctgc tcgtgccggc gatccgccct cccaccgtcg
     2341 ccgtcggagc gtcgaggctg cgcatcgccg tctccgcggc gcacacaccc gccgacgtcg
     2401 ccgagctgat cgaagcgctc ggcgctctgg gggtcacgct cgacgccgag gtcgcggcat
     2461 gagcgagcga ccgcgccggc tggtcgtcgt cgccggcacg gcgaccgaga tcggcaagac
     2521 gtgggtgagc gctgcgctcg ccacggaact ggtggcggat cactgggccg tggaggcccg
     2581 caagccggcg cagtccggcg gtcccggcga cccgagcgac gccgatctcc tggccgcggc
     2641 gacggaggaa gcgtcgacca cggtgtgccc gccgcatcgt cggtactcgg tgccgatggc
     2701 accgccgatg gccgccgagg cgacgggtct ggaaccaccg acgatcgccg agctggtggg
     2761 tgaggtcgcg gcctcgtggg ggccgcgggc cgccgacgtg gggctcgtcg agttggcggg
     2821 tggggtggcg tcgccggcgg gcgccgacgg cgatggtgcc gatctcgcag cggcgctcgc
     2881 tcctgacgcg gtggtgctcg tcgccgacgc cgggttgggc acgatcaacg ccgtccgcct
     2941 ctcgctcgcg cggctcgccg tggtcgggtt gaacgcagtg gtgtacctca atcgctacga
     3001 cgacggcgac gatctgcacc gccgcaaccg ggcctggctg gccgaccgtg acggcctgtc
     3061 ggtgctgacg tcgatctcgg cgctggtgga gtggacgctc ggccgcgcac cgctgcattg
     3121 cggctactgc ggcaagccgg tgaccgagtg cgacagca
//
LOCUS       DAOSOA010000360         3153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_30785_length_3153_cov_4.265332, whole genome shotgun sequence.
ACCESSION   DAOSOA010000360 DAOSOA010000000
VERSION     DAOSOA010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30785_length_3153_cov_4.265332"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PKY13_15725"
     CDS             complement(<1..926)
                     /locus_tag="PKY13_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81442.1"
                     /translation="MNDWPELLALAERQYGLVTREQIRRLGGSQAAVLRVARSSQWEP
                     VTKQVLRRRGSPRTVDQMVMAAVLDAGGDATLAYLPGAAWWGVAGCVLDPLVLCTTKK
                     ARTRTGLATTHEVRWLPDRWVTELRGVRVARPELLAMQLHAICRPERAEVLVDRMWSR
                     RLLSGPSIAQFIDEAGASGRNGITGLRAYFQPRGLKYIPPASGLEGRVKGLLGDAGIP
                     MRPQVDSGGERWTGRVDFRHVDLPFIVEVQSEAHHAALVDQVADAVRIDQLVADGFSV
                     VEISDDEVWLTARETVRHVEDCLRESIANGFAL"
     gene            complement(1061..2155)
                     /locus_tag="PKY13_15730"
     CDS             complement(1061..2155)
                     /locus_tag="PKY13_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOT81443.1"
                     /translation="MKELLVELGHWWERGERSVLARVVDTEGSGPRDAGAAMAVNESG
                     EVIGSVSGGCVESAVVTEALDVMATQRPRVVSFGYSDEDAFAVGLTCGGTVQLYLEPW
                     EPTTIAEELARGIAARRPLALVTVIDGVGTGRQMLVSPDSPDLGDLGDPELNRVAGRD
                     ALGELDAGTSSVRHYGRSGENGEDAVTVFIESFASPPRMLIFGAVDFTGALSRAAKFL
                     GFHVIVCDAREVFATAARFPAADEIVVDWPHRYLASIADELGPRDAICVLTHDNKFDV
                     PALIEAFGTPVGYIGALGSRRTAATRREQLVAAGASDASLMRLNAPIGLDLGGRSPEE
                     TAISIAAEIIAQRNGRTASSLRNTGGPIHR"
     gene            complement(2207..>3153)
                     /locus_tag="PKY13_15735"
     CDS             complement(2207..>3153)
                     /locus_tag="PKY13_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81444.1"
                     /translation="CRELLGSAAGANIFMIGVAVQSGALPVGPGPIEAAIELNGVAVD
                     ANLAAFRWGRWWVADPSRLGTPAGGADHHDMWVPDLPPALGRRIDALGPLEADGSLDA
                     LLRLLAADLVGYQSEAYAGRFLDRIDAALAAERQVDPESVVFTEATARSLHQLMAYKD
                     EYEVARLLLGPEGAATAEIVGGPDATVTWKLHPPTLAALGMGTKLSVPARMVAPAMKA
                     LARGRRLRGTPVDPFGRTEVRRTERRILADLEAAIDQLTRSLAADPTPERLAAATELV
                     SLARSVRGYERLKLDRAATFSRELAAGLAAFRATERSR"
BASE COUNT          502 a         1141 c         1067 g          443 t
ORIGIN      
        1 agggcaaaac cgttcgcgat ggactcgcga aggcagtcct cgacgtggcg aacggtttcg
       61 cgggccgtca gccacacctc atcgtcggag atctcgacca ccgagaagcc atcggccacg
      121 agctggtcga tgcggaccgc gtccgcaacc tggtcgacga gggcagcgtg gtgggcctca
      181 ctctggacct ccacgatgaa cgggaggtca acgtgacgga agtcgacccg accggtccag
      241 cgctcgcctc cggagtcgac ctgtggccgc atcgggatcc cggcatcccc gagcaatccc
      301 ttgacccgcc cctcgagccc cgacgccgga gggatgtact tcaggccccg tggctggaag
      361 taggcccgaa gaccggtgat gccattgcgt ccgctcgctc cggcctcgtc gatgaactga
      421 gcgatcgacg gccccgacag gagtcgtcgg ctccacatcc gatcgacgag cacctcagct
      481 cgctccggcc ggcagatcgc atgcaactgc atcgccagga gctcgggacg agcaacccga
      541 accccccgca gctctgtcac ccaccggtcg gggagccagc gcacctcgtg agtcgtcgcc
      601 agacccgtcc gcgtccgagc cttcttggtg gtgcacagga cgagcgggtc gagcacacac
      661 ccggcaactc cccaccatgc tgcgccgggg aggtacgcca aggtggcatc ccctccggca
      721 tcgagcaccg cagccatcac catctgatcg acggtcctcg gggacccgcg ccgccgcagc
      781 acctgcttcg tcaccggctc ccactgtgac gatcgggcga ctcgtaggac tgcagcctgc
      841 gacccgccga gccgccggat ctgctcgcgg gtcaccagcc cgtactgccg ttccgcgagg
      901 gcaaggagtt cgggccagtc gttcatgcga cccgagaatg gggccgcccg tgctcgctcc
      961 accatggggg acgtccccct cgagttttgc cctgcagttc aatcggcaaa gcggtcgatc
     1021 tgcagggcag aacccggggg gaggggccgc ggatcgtccg tcagcggtgg atggggcctc
     1081 cggtgttgcg gagggagctc gccgtccggc cgttgcgctg ggcgatgatc tccgctgcga
     1141 tcgagatggc ggtctcttcg ggcgaccgac cgccgagatc gaggccgatc ggagcgttca
     1201 gacgcatgag cgacgcgtcc gatgcacccg ccgccacgag ctgttcccga cgtgtggccg
     1261 cagtgcgacg gcttcccaac gcaccgatgt agccgaccgg cgtgccgaac gcctcgatga
     1321 gtgccggcac gtcgaacttg ttgtcgtggg tgagcacaca gatcgcgtcg cgcggaccca
     1381 gttcgtcggc gatcgaggcc aggtagcgat ggggccagtc gaccacgatc tcgtcggcag
     1441 ccgggaaccg ggccgccgtg gcgaacacct cgcgtgcatc acacacgatc acgtggaacc
     1501 ccaggaactt cgcggcacga ctcagtgcac ccgtgaagtc gaccgctccg aagatcagca
     1561 ttcgaggcgg cgaggcgaac gactcgatga agacggtgac cgcatcctcg ccgttctcgc
     1621 cgcttcgccc gtagtggcgg acgctgctcg tgccggcgtc gagctcgccc aacgcatcgc
     1681 gtcccgcgac gcggttcagt tcgggatcgc cgaggtcgcc gaggtcgggg gagtcgggcg
     1741 acaccagcat ctgccgaccg gtcccgacgc cgtcgatgac ggtgacgagc gccagcgggc
     1801 ggcgcgccgc gatcccgcgc gccaactcct cggcgatcgt cgtcggctcc cacggttcga
     1861 ggtagagctg aaccgtgccg ccgcaggtca ggcccacggc gaacgcgtcc tcgtcgctgt
     1921 acccgaagct gacgactcgg ggccgctgcg tcgccatgac atcgagcgct tcggtcacga
     1981 cggcactctc gacgcacccg cccgagaccg aaccaatgac ctcgcccgac tcgttcaccg
     2041 ccatcgccgc accggcgtcc cggggacccg agccctcggt atcgacgacg cgggcgagca
     2101 ccgaccgctc gccacgctcc caccagtggc cgagctcgac caggagttcc ttcatcggat
     2161 ccaggctaac ggtcgtgcac gccggctccg ggaggtcgcg gagaactcac cgtgaacgct
     2221 ctgttgctcg gaaggcggcc aacccggcag ccagctcgcg gctgaacgtg gcggcgcggt
     2281 ccagcttgag gcgctcgtag ccgcggaccg aacgggcgag gctcacgagc tcggtcgcag
     2341 cggcgagacg ttcgggcgtc ggatcggccg ccaacgaccg ggtcagctga tcgatcgccg
     2401 cctcgaggtc ggccaggatc cgtcgttcgg tgcgccggac ctcggtgcga ccgaacgggt
     2461 cgacgggcgt gccccgcagt cgccgaccac gagcgagcgc cttcatcgcc ggagcgacca
     2521 tcctcgccgg cacgctcagc ttcgtcccca tcccgagggc cgcgagggtc ggcgggtgga
     2581 gcttccacgt gacggtggcg tcggggccac ccacgatctc cgccgtcgcc gcgccctcgg
     2641 ggccgaggag cagccgggcg acctcgtact cgtccttgta cgccatgagc tggtgcaggc
     2701 tgcgcgctgt cgcttcggtg aagacgaccg actccgggtc gacctggcgc tctgccgcga
     2761 gcgcggcgtc gatccgatcg aggaagcggc cggcgtacgc ctcggactgg tagcccacga
     2821 gatcggcggc gagcaatcgg aggagggcgt cgagcgagcc gtcggcctcg agcgggccga
     2881 gcgcgtcgat ccgccgaccg agcgccggcg gcaggtccgg cacccacatg tcgtggtggt
     2941 ccgctccgcc ggccggtgtg cccaggcggg acggatcagc gacccaccac cgaccccacc
     3001 ggaacgcggc caggttcgcg tccaccgcca cgccgttgag ctcgatggca gcctcgatgg
     3061 ggcccggccc gacgggcagc gcgcccgact gcaccgctac accgatcatg aagatgttgg
     3121 cgccggcggc cgatcccagc agctcccgac acc
//
LOCUS       DAOSOA010000361         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_30816_length_3150_cov_4.871729, whole genome shotgun sequence.
ACCESSION   DAOSOA010000361 DAOSOA010000000
VERSION     DAOSOA010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30816_length_3150_cov_4.871729"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PKY13_15740"
     CDS             complement(<1..876)
                     /locus_tag="PKY13_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT81445.1"
                     /translation="MTELSRIRNLSIIAHIDHGKSTLADRILEITGAVDARDMRAQYL
                     DSMDLERERGITIKLQSVRLDYKDHVIHLIDTPGHVDFGYEVSRSLAACEGVILVVDA
                     SQGIEAQTLANCYLALENDLEIVACLNKIDLPAAEPDLYAAEIEQILGIPADDILRIS
                     AKTGEGVAELLDAVVERIPAPTGEIDEPLQALIFDSHFDSYRGVVSSVRVMNGQLRTG
                     SKLKFMQAGVTHEAIEVGGRRPENTPVPALGPGEVGYLIAGIKDVGEARSGETITEAA
                     RPAAEPLDGYMEPKPM"
     gene            1051..1326
                     /gene="rpsT"
                     /locus_tag="PKY13_15745"
     CDS             1051..1326
                     /gene="rpsT"
                     /locus_tag="PKY13_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOT81446.1"
                     /translation="MANIKSQIKRNKQNEKARLRNKAVRSNLKTRIKSAETAAANGAE
                     ESEATLRTAISTIDRAATKGVIHKNAAARKKSRLVKRLARLSEDAAS"
     gene            complement(1424..2437)
                     /gene="holA"
                     /locus_tag="PKY13_15750"
     CDS             complement(1424..2437)
                     /gene="holA"
                     /locus_tag="PKY13_15750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOT81447.1"
                     /translation="MNEPIILLKGSDPVLLADAAAARLSVLLGDRDHDEVVDMFSGDD
                     FDLSDAVLAAQAVSMFGDRVIVVRNASRFNVEALGPLIAYADDPNPTSRVLVVWDKPM
                     SPSGHAHAVPKKLTDAVKACGGVVSDSDVSPNAKVRQGWLDEHLAAAAVKFLPATKSL
                     IIERLGEDVSRLGGVIRLLEATYPPGSKLSPDEVEPFLGEAGSVPPWELTDAIDKGDV
                     ALAVSKLRRMMSAGERHPLAIMSTLGTHFQRMLRLDGAGVRNEQEAADLLGMKKGSTF
                     PAKKAMQQGRNLGSDRLARAVRLLARADVELRGATAVPSEAVMEVLVARLAQLSRGGS
                     TRR"
     gene            complement(2434..2673)
                     /locus_tag="PKY13_15755"
     CDS             complement(2434..2673)
                     /locus_tag="PKY13_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81448.1"
                     /translation="MPAEPAPDSTDHGHTPPDPDVAVPAGVGTTIAGWTIAAMAGSDV
                     VAGSLAAVEVRRLRRVQAAVAALRASAPRSAGKPA"
     gene            2792..2947
                     /locus_tag="PKY13_15760"
     CDS             2792..2947
                     /locus_tag="PKY13_15760"
                     /inference="COORDINATES: protein motif:HMM:NF040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HOT81449.1"
                     /translation="MITAGLILLLVGFIAKISILWSLGILLLVIGAVLMVMGQLGREV
                     GGRRHYF"
BASE COUNT          494 a         1092 c         1076 g          488 t
ORIGIN      
        1 catgggcttc ggttccatgt agccgtcgag cggctcggcg gcgggacgcg cagcctcggt
       61 gatggtctcg ccggagcggg cctcgccgac gtccttgatg ccggcgatga gatagccgac
      121 ctcaccgggg ccgagcgccg ggaccggggt gttctcgggc cgccggcccc cgacctcgat
      181 cgcctcgtgg gtcactccgg cctgcatgaa cttgagcttg gagccggtgc ggagctggcc
      241 gttcatgacc cgcaccgagc tgacgacacc tcggtagctg tcgaagtgcg agtcgaagat
      301 cagcgcctgc agcggctcgt cgatctcacc cgtcggggcc gggatgcgct cgaccacggc
      361 atcgagcagc tcggcgacgc cctcaccggt cttggcggag atacggagga tgtcgtcggc
      421 ggggatcccg aggatctgct cgatctctgc ggcgtagagg tcgggttcgg ccgcgggcag
      481 gtcgatcttg ttgagacacg ccacgatctc gaggtcgttc tcgagtgcca ggtagcagtt
      541 ggcgagggtc tgcgcctcga tgccctgact ggcgtcgacg accaggatca cgccctcaca
      601 cgccgccagc gagcgggaca cctcgtaacc gaagtcgacg tggccgggcg tgtcgatgag
      661 gtggatgacg tggtccttgt agtcgagccg gaccgactgc agcttgatcg tgataccacg
      721 ctcgcgttcg aggtccatcg agtcgaggta ctgggcccgc atgtcgcgcg cgtcgacggc
      781 gccggtgatc tcgaggatgc ggtcggcgag ggtcgacttg ccatggtcga tgtgggcgat
      841 gatcgacagg ttgcggattc gggagagctc ggtcacgacg atgccacgct agcaagcctg
      901 cggggacacc cgatcgggcg gagatgacgt gccgccgtcg aaccactaca ctcgttgagt
      961 cgtgtgtcgg ccctcgcgcc gcgccgcccg ttcatccccc ggatcccgca ccgctcacgc
     1021 accgctgatc ccgagactgc aaggttcacc gtggccaaca tcaagagcca gatcaagcgc
     1081 aacaagcaga acgagaaggc ccgccttcgc aacaaggctg tgcgttccaa cctcaagacg
     1141 cgcatcaaga gcgccgagac ggccgctgcg aacggtgccg aggagtccga ggccacgctg
     1201 cgcacggcca tctccaccat cgaccgtgcc gccaccaagg gcgtcatcca caagaacgct
     1261 gcggcccgca agaagtcccg cctcgtgaag cgcctggcgc gcctctccga agacgccgcc
     1321 tcctgagtcg tcgtcctccg gacaaccgaa gttcaccgaa cgagccgcct tcgggcggct
     1381 ccttcgcgtc ccgacaccgt cggttctgct gcggcaccgg ttgctagcgg cgggtcgatc
     1441 cgccgcggga gagctgggcg aggcgggcca ccaggacctc catgaccgct tcggacggca
     1501 cggccgtcgc tccccgtagc tcgacgtcgg cgcgggccag caaccggacg gcccgggcga
     1561 gacgatcgga gccgaggttg cgcccctgct gcatggcctt cttggcgggg aacgtcgacc
     1621 ccttcttcat gccgaggaga tcggcggcct cctgctcgtt gcggaccccg gcgccgtcga
     1681 gccggagcat gcgctggaag tgggtgccga gggtcgacat gatggccagc ggatggcgtt
     1741 cgcccgcgct catcatgcga cgcagcttcg acaccgccag tgcgacgtcg cccttgtcga
     1801 tggcatcggt cagctcccac gggggcaccg accccgcctc gccgaggaac ggttcgacct
     1861 cgtcggggct gagcttggaa ccgggcggat aggtcgcctc caacagacgg atcaccccgc
     1921 cgagccggga gacgtcctcg cccaggcgtt cgatgatgag cgacttggtc gccggcagga
     1981 acttcacggc ggccgcggcc aggtgctcgt cgagccaccc ctgccgaacc ttcgcgttgg
     2041 gcgacacgtc gctgtcggac accacaccgc cgcacgcctt caccgcgtcg gtgagtttct
     2101 tcggcacggc gtgggcgtgc cccgacgggc tcatcggttt gtcccagacg acgaggacgc
     2161 gcgaggtcgg gttgggatcg tcggcgtagg cgatgagtgg tccgagcgct tcgacgttga
     2221 accgcgaggc gttgcgaacg acgatcacac gatcaccgaa catggagacc gcctgcgccg
     2281 cgaggacggc gtcggagagg tcgaagtcgt caccggagaa catgtccacg acctcgtcgt
     2341 ggtcacgatc accgagcaac acggacagcc gggccgcggc ggcatcggcc aacagcaccg
     2401 ggtcggagcc cttcagcagg atgatcggtt cgttcacgcc ggcttcccgg cggatcgtgg
     2461 agcgctcgcc cgcagcgcgg cgacggcggc ctgcacgcgg cgcagtcggc gcacctcgac
     2521 cgccgccaac gacccggcga cgacgtcgga gcccgccatc gccgcgatcg tccacccggc
     2581 aatcgtcgtg ccgacacccg cgggaacggc cacgtcgggg tcgggagggg tgtggccgtg
     2641 gtcggtggag tcgggagcgg gctcggcggg cacgccccga cgctagccgg gtgccaccga
     2701 caccgaatcc ggcagatccc ccgcccgggt ggttccaccg gcgtgccggc ggggtatcga
     2761 tcgcccgtcc agctacacgg aggttcatcc catgatcacc gcaggtctca tccttctcct
     2821 cgtcgggttc atcgccaaga tctcgattct gtggtccctc ggcatcctgc tcctggtgat
     2881 cggagcggtg ctcatggtca tgggccagct cggccgcgag gtcgggggtc gacgtcacta
     2941 cttctgaccg acaacccacc tgacccggag gcgtcgccgg gtggccgcgt gatctcgatc
     3001 cgcaggctcc cggcggcgac cgtcccctcg aggagtggtg ccgcatcccg aatcgatgcc
     3061 gggtccgccg ccagcaccgt cccgacctcg accacctgac gcagcgagta caccgctccc
     3121 gacgacgtct gcccgccccc ggtgacgatg
//
LOCUS       DAOSOA010000362         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_31493_length_3101_cov_3.992449, whole genome shotgun sequence.
ACCESSION   DAOSOA010000362 DAOSOA010000000
VERSION     DAOSOA010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31493_length_3101_cov_3.992449"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PKY13_15765"
     CDS             <1..1119
                     /locus_tag="PKY13_15765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT81450.1"
                     /translation="APVLSSGLGVSATDFGIAVSVIRVSGLAAVVLVGLADRLGRRHM
                     LLVTLAGLAVSTGLTAAAWGIVSFVVLSSLSRVFLSAEGVMTGVVIAEEVHPTRRGRA
                     ISVSGLIGQTGFGAVAILVAVVPSLPLGWRWLYLLALGPLLLVAGLRRNLSETEAFHA
                     AASEDRVHRRGVLRLPRRWWGRTAACVVVFGAVGAFQTSAGYYAAQLAQNTYDWTGTY
                     TLVVIASGLFTLLGFIVGGRGSDRVGRRPVISLGVVVETAGVAVMFGGGESWYAVGWF
                     GFALGQAMIAGCWVAFVSELVPTEVRATVSSLVVSMQVAAGSVGLVVASTVGESIRES
                     GRVGAWMGVVSIASLGVLWRLPEVAGRDVVGAYGPEHR"
     gene            1433..>3101
                     /locus_tag="PKY13_15770"
     CDS             1433..>3101
                     /locus_tag="PKY13_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81451.1"
                     /translation="MYLLVDADLGETARHVVGLLDPVIGGTADLSIAVFPSAGGRGGF
                     GVVKRAARSIIARECGYDAVEPLSGQRAVRGDLLRSLPAAPRFGLEVGMTVDAVRAGA
                     RLVEIEMPLDHEHTGRTLAGFRHRGGQAVDIVRAAAPRLGSVPVRAALMVVGTLAVLV
                     GILAAPWAAGSGRPLVAASGRKVVVVTVADVSLADLDRGVLPNISNLLGTTGGALTPR
                     TPAAPGDQLSSYASLGAGAPVVVADGRPGPVRALVRPTIILTPDGDGARLAEPVAVTG
                     ARNAYGTLGALGDALHGAGLRTGYVGARSGGDGLDAPGALAVADSSGAVDVALAGSLP
                     EAEGPDLDAALGDRVAAVGTAMASSDVVLVDAGGSPLVQPAWLGPSTAAAREDRHQRR
                     LGHVRVADLLVGRIVAANPGALVVVAGVAPPSQWRLTPLAVAGDLPGALSSPSTRRGG
                     LAALTDLGPTVLAAFGIDRPDTMIGQRLTSGPDAADVAAVRELHDRTDARERLNPWIT
                     VGFILLQAVVYGAAMVGLATRRRWGRPVERLLVVAERVALACAAFPVV"
BASE COUNT          310 a         1088 c         1179 g          524 t
ORIGIN      
        1 gcgccggtgt tgagcagcgg actcggcgtg agcgcgaccg atttcggcat cgccgtgtcg
       61 gtcatccggg tctcgggcct cgctgcggtg gtgctcgttg gtctggcaga ccggttgggg
      121 cggcggcaca tgctcctcgt gaccctggcc ggtttggccg tgtccacggg cctgaccgcc
      181 gcggcgtggg gcatcgtcag cttcgtcgtg ctcagttcgt tgtcccgggt gttcctgtcc
      241 gccgagggcg tgatgaccgg cgtggtcatc gccgaggagg ttcatccgac ccggaggggc
      301 cgcgccatct cggtgtcggg gctcatcggc cagaccgggt tcggcgccgt cgcgatcctc
      361 gtggcggtgg tcccgtcgtt gcccctcggc tggcggtggc tctacctcct cgcgctcggc
      421 ccgttgctgc tggtggccgg actgcgccgg aatctcagcg agaccgaggc cttccatgcc
      481 gcggcgtccg aggaccgtgt ccatcggcga ggagttctcc gtctcccgcg ccgttggtgg
      541 ggtcggaccg cggcgtgtgt ggtcgtgttc ggagccgtgg gcgcgttcca gaccagcgct
      601 gggtactatg ccgcccagct cgcccagaac acctacgact ggaccggcac gtacacgctg
      661 gtcgtgatcg cctcggggct gttcacgctg ctggggttca tcgtcggcgg gcgggggagc
      721 gaccgcgtcg gtcgtcggcc cgtcatctcc ctgggagtgg tggtcgagac ggcgggcgtc
      781 gccgtgatgt tcggcggcgg cgaatcctgg tacgcagtcg gctggttcgg gttcgcgctc
      841 ggtcaggcga tgatcgccgg gtgctgggtg gcgttcgtgt ccgagctggt cccgaccgag
      901 gttcgggcga cggtcagctc gctcgtcgtc tcgatgcagg tcgctgccgg gtccgtcgga
      961 ctggtggtgg cgagcaccgt cggcgagtcg atccgggagt cgggccgggt cggcgcctgg
     1021 atgggcgtgg tgagcatcgc ctcgctcggt gtcctgtggc ggctccccga ggtcgccggc
     1081 cgcgacgtcg tcggtgcgta cggccccgag catcgctgac gatacttttc tcgcgttctc
     1141 gtccagcatg gcgctcccgg cgacgagtgg gggtgatgcg gatgggggac gaaacggtgc
     1201 ccgatgcggt gatcgcggtg gttccggcga agaactccgg ggcgatcggg ccgacggtcg
     1261 ccgcgctgtg cgcaacgggc cgggtggacc gcgtcgtcgt ggtggatgac ggatcgaccg
     1321 acgacactgc ggaacgggcg gcggccgccg gggcgacggt gctctccctc gccacgaact
     1381 ggggaaaggg ggtgccgtcg cacgggctgt cgagcagttc cccgaggcgg cggtgtacct
     1441 gctcgtcgac gccgacctcg gcgagacggc gcgtcacgtc gtcggtctgc tcgacccggt
     1501 gatcggcggc actgccgatc tctcgatcgc ggtgttcccg tcggccggtg ggcggggcgg
     1561 gttcggtgtg gtcaagcggg cggcccgctc gatcatcgcc cgggagtgcg gctacgacgc
     1621 cgtggagccg ttgtcgggcc agcgcgccgt gcggggcgac ctgctccggt cactcccggc
     1681 agcgccacgc ttcggcctcg aggtcgggat gaccgtcgat gccgtccggg ccggcgcccg
     1741 actcgtcgag atcgagatgc cgctcgacca cgagcacacc ggacggacac tcgccggctt
     1801 ccgccaccgt ggcggccagg ccgtcgacat cgtgcgggcg gcggcccctc gtctcggatc
     1861 cgtgccggtc cgcgccgcgc tgatggtcgt cggcacgctc gccgtgctgg tcggcatcct
     1921 ggcagcgccg tgggcggcgg gcagcggtcg accactcgtg gcggcgtcgg ggcgcaaggt
     1981 ggtcgtcgtc accgtggccg atgtcagcct ggccgacctc gaccgtggtg tgctccccaa
     2041 catctcgaac ctcctcggca cgacgggcgg tgcgctcacg ccgcgcaccc cggccgcgcc
     2101 gggcgaccag ctgtcgagct acgcgtcgct cggcgccggt gcccccgtgg tggtcgccga
     2161 tgggcggccc ggtccggtgc gtgcgctcgt gcgtcccacg atcatcctca cccccgacgg
     2221 tgacggcgcc cggctcgccg aaccggttgc cgtgaccggt gcgcgcaacg cctacggcac
     2281 gctcggcgcg ctcggtgacg cgctgcacgg ggcggggctt cgcaccggtt acgtgggcgc
     2341 ccgttcggga ggcgacgggc tcgacgctcc cggcgcgctc gccgtcgccg actcgtcggg
     2401 cgccgtcgac gtcgcgctgg ccggttcgct cccggaggct gaaggtcccg atctcgacgc
     2461 cgccctcggt gatcgtgtcg ccgccgtcgg aacggccatg gcgtcgagcg acgttgtgct
     2521 cgtcgacgcc ggcggttcgc ccctcgtcca accggcgtgg ctcggcccgt cgacggcggc
     2581 ggcgcgcgag gaccgccacc agcgccggct cggtcacgtc cgcgtcgccg acctgctcgt
     2641 cggccgcatc gtcgccgcga acccgggagc gctcgtcgtc gtggcgggtg tcgccccgcc
     2701 gtcgcagtgg cgcctcacac cgctggccgt agccggcgat ctccccggtg cgctgtcgag
     2761 tccctccacc cgtcgtggtg gcctcgccgc gctcaccgac ctcggtccga cggtgctcgc
     2821 cgcgttcggc atcgaccggc ccgacacgat gatcgggcag cgcctcacgt ccggaccgga
     2881 cgccgccgac gtggccgccg tccgggagtt gcacgaccgc accgatgccc gggaacggct
     2941 gaacccctgg atcacggtgg ggttcatcct cctccaggcg gtcgtgtacg gcgccgcgat
     3001 ggtcgggctc gcaactcgtc gccgctgggg ccgtcccgtc gaacgcctgc tcgtcgtggc
     3061 cgaacgggtc gcgctggcgt gcgctgcgtt cccggtcgtg a
//
LOCUS       DAOSOA010000363         3074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_31825_length_3074_cov_6.002319, whole genome shotgun sequence.
ACCESSION   DAOSOA010000363 DAOSOA010000000
VERSION     DAOSOA010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31825_length_3074_cov_6.002319"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..777
                     /gene="trmD"
                     /locus_tag="PKY13_15775"
     CDS             34..777
                     /gene="trmD"
                     /locus_tag="PKY13_15775"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT81452.1"
                     /translation="MRIDVFSIFPEMITDFGGHSLLGKAQEKQLLDLRAHDLRAHTTD
                     PHRTVDDSPFGGGAGMVLMPEPIFAAVEAVDPPRPLLMLSPAGRRFDQTMAEELAAGP
                     GFSLLCGRYEGIDQRVRDHLCDGEVSLGDFVLNGGEVAAFAIIEAVARLVPGVMGNSS
                     SALDESFSSGLLEYPQYTRPAEFRGWAVPDVLRSGDHGRVDRWRRAQALARTLRDRPD
                     IVAAAGGLGDADRRLLADHGFDEAGHPVG"
     gene            893..1234
                     /gene="rplS"
                     /locus_tag="PKY13_15780"
     CDS             893..1234
                     /gene="rplS"
                     /locus_tag="PKY13_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOT81453.1"
                     /translation="MKPTDVVDQSSLRDDVPDFAPGDTLKVHVRVVEGTRERVQVFQG
                     AVIRRQGGGLQETFTVRKLSFGVGVERTFPVHSPTISKIEIVTRGDVRRAKLYYLRDR
                     VGKSAKIKEKR"
     gene            1327..1686
                     /locus_tag="PKY13_15785"
     CDS             1327..1686
                     /locus_tag="PKY13_15785"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOT81454.1"
                     /translation="MSQHRRSLGIEGEELAARWYERHGYVVAERNWRCRAGEIDLILR
                     RGREVVVCEVKTRSSDRYGTGAAAVDWRKQRTIRRVTAAYLSQREGRAPSGIRFDVAV
                     VSPGPHGWAIEVIEHAF"
     gene            complement(1697..1831)
                     /locus_tag="PKY13_15790"
     CDS             complement(1697..1831)
                     /locus_tag="PKY13_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81455.1"
                     /translation="MAARYWGPMGFNPNRVHKKRASDYVLVAAAVAICIAALIWALLG
                     "
     gene            1830..2597
                     /locus_tag="PKY13_15795"
     CDS             1830..2597
                     /locus_tag="PKY13_15795"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HOT81456.1"
                     /translation="MDDSSHQPQDYIGFDPAASAPPPEQLRLSPEETRVLGCLIEKSR
                     TTPDDYPLTGNALMRACNQSTSRDPIVNYDQRTIDATVNSLKSAGLLRFVHTTSGRNI
                     TRYRHVVDERLGLDEAETAIFGLLMLRGAQTVAELKTRAERLHPFRSLDDVNATLRSL
                     TVRPEPFAVLLERQPGQKEARWTHVLYGEPLLPSATPSASSGGAGGGGATSARIAELE
                     AQVAALTSALNEVRTELGLDPIATGPAATEGAADGSA"
     gene            complement(2681..2926)
                     /locus_tag="PKY13_15800"
     CDS             complement(2681..2926)
                     /locus_tag="PKY13_15800"
                     /inference="COORDINATES: protein motif:HMM:NF017691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HOT81457.1"
                     /translation="MKRSLAIAAILVSLSVTTSCKFSNCAALNATHPHGIGRPGAVDK
                     VAGGAQRVTNFTVDAAQYNENASKLDRDRDGIACEKL"
BASE COUNT          448 a         1064 c         1061 g          501 t
ORIGIN      
        1 cgcttcgctc cggtacctct gcggggcagc ctgatgcgga tcgacgtgtt cagcatcttc
       61 cccgagatga tcaccgactt cggtggccat tccctcttgg gtaaggccca ggagaagcag
      121 ctcctcgacc tccgtgccca cgacctccgg gcgcacacga ccgatccgca ccgcacggtc
      181 gacgactcgc cgttcggcgg tggcgccggc atggtgctga tgcccgagcc gatcttcgcg
      241 gcggtcgagg ccgtcgatcc gccgcggccg ctcctgatgc tgtcgcccgc cggccgccgg
      301 ttcgaccaga ccatggccga ggagctggcc gccggtccgg gcttctcgct cttgtgtggg
      361 cgatacgagg gcatcgacca gcgggtccgc gaccacctgt gcgacggcga ggtgtcgctc
      421 ggcgacttcg tcctcaacgg gggagaggtg gcggccttcg ccatcatcga agcggttgct
      481 cgcctggttc cgggggtgat gggcaactcc agttcggcgc tcgacgagtc cttcagctcg
      541 ggcttgctcg agtacccgca gtacacccgt cccgccgagt tccgcggctg ggccgtgccc
      601 gatgtgttgc gttcgggcga ccacggccgc gtcgaccggt ggcgtcgcgc ccaggcgctc
      661 gcccgcacgt tgcgtgaccg ccccgacatc gtcgctgcgg ccggcgggct cggcgacgcc
      721 gaccgccgtc tgctcgccga ccacggattc gacgaggctg gacaccccgt cggctaggga
      781 tgttggtgcc acggactggg agcgagtaac gtcactccgt cgcgtcccgc ctggacgccc
      841 gcgccccacc gggccgcact tcttcgaaat ccacctcagg agcacacgcg ccatgaagcc
      901 gaccgatgtt gtcgaccagt cgagcctgcg ggacgacgtc cccgatttcg ctcccggcga
      961 caccctcaag gtgcacgtcc gcgtcgtcga gggcacccgt gagcgtgtcc aggtgttcca
     1021 gggtgcggtc atccgtcgcc agggtggcgg cctgcaggag accttcacgg ttcgcaagct
     1081 gtccttcggc gtcggcgtcg agcgcacctt cccggtgcac tccccgacga tctcgaagat
     1141 cgagatcgtg acccgtggcg acgtccgccg cgccaagctg tactacctgc gcgaccgcgt
     1201 cggtaagtcg gcgaagatca aagagaagcg ctagtcctca gcggcgcact gcgccgctga
     1261 ctcacagctt cccctcccgg tcacacatga cggcgcctgc tggttgcagc gcgcccggga
     1321 ggcaacatgt cccagcaccg acgatccctc gggatcgagg gtgaggagtt ggcggcgcgc
     1381 tggtacgaac gccacggcta cgtcgtcgcc gagcgcaact ggcgttgccg cgccggagaa
     1441 atcgacctga tcctgcggcg cggccgcgag gtcgtggtgt gcgaggtgaa gacccgctcg
     1501 tcggatcgtt acggcaccgg tgccgctgcg gtggactggc gcaagcagcg cacgatccgc
     1561 cgggtcaccg cggcgtacct ctcacagcgc gagggccgcg ccccgtcggg catccggttc
     1621 gatgtcgcgg tggtatcgcc gggtccacac gggtgggcga tcgaggtgat cgagcacgcg
     1681 ttctgagcgt cgccgatcag ccgagcagcg cccagatgag cgcggcgata cagatggcga
     1741 cggcggcggc gaccagcacg tagtcgctgg cgcgcttctt gtggacgcgg ttggggttga
     1801 agcccatggg tccccagtat cgtgcggcca tggacgactc gtcgcatcag ccccaggact
     1861 acatcggctt cgaccccgcg gcgtcagctc cgccgcccga gcagctccga ctgtcgcccg
     1921 aggagacgcg ggtgctcggt tgtctgatcg agaagtcgcg gacgactccc gacgactatc
     1981 cgctcaccgg caacgcgctg atgcgggcgt gcaaccagtc gacgtcgcgg gacccgatcg
     2041 tcaactacga ccagcggacg atcgacgcga ccgtcaactc gttgaagtcg gcgggcttgc
     2101 tgcggttcgt ccacaccaca tcgggtcgca acatcacccg ttaccgccac gtcgtcgacg
     2161 agcggctcgg actcgatgag gccgagacgg ccatcttcgg tctgttgatg ctccgtggcg
     2221 cccagacggt ggcggagctc aagactcggg ccgagcggct gcacccattc cgatcgctcg
     2281 acgacgtgaa cgccacgctg cgttcgttga cggtgcgacc tgagcccttc gccgtgctgc
     2341 tcgagcgtca gcccggccag aaggaggcgc gctggaccca cgtgctctac ggggaaccct
     2401 tgctcccgtc ggccaccccc tcggcgtcct cggggggcgc gggcggcggt ggcgcgacct
     2461 cggcccgcat cgccgaactc gaggcgcagg tcgcggccct gacctcggcg ttgaacgagg
     2521 tgcgaaccga actcggtctc gacccgatcg cgactggccc tgccgccact gagggcgcgg
     2581 ccgacgggtc cgcctgatcg agcaccggac ccgggcgact cggcccgggc gacacgacga
     2641 tcaacgaacg ggcgagcgct gacgcagtct gcccggcgag ctacagcttc tcgcaggcga
     2701 tgccgtcgcg atcgcgatcg agcttcgagg cgttctcgtt gtactgcgcc gcgtcgacgg
     2761 tgaagttcgt gacccgctgg gccccgcccg cgaccttgtc gacggccccg gggcggccga
     2821 tgccgtgcgg atgcgtggcg ttcagcgccg cgcagttgga gaacttgcag gacgtcgtga
     2881 ccgagagcga gacgaggatg gcggcgatgg cgagggagcg cttcatcccg ccagggtaga
     2941 tcattttcgt tttgaactag ccggaagttg gtgcttcgat cactcccgac gcgctggcga
     3001 gggtgccgtt ctcggtgtcg ggggtgggcg ctaggttgct gacacgcccc tgaggggcgg
     3061 atcgcccgcc ggtt
//
LOCUS       DAOSOA010000364         3071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_31869_length_3071_cov_4.510942, whole genome shotgun sequence.
ACCESSION   DAOSOA010000364 DAOSOA010000000
VERSION     DAOSOA010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31869_length_3071_cov_4.510942"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKY13_15805"
     CDS             <1..538
                     /locus_tag="PKY13_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOT81458.1"
                     /translation="LEVPRHVMVQVAEPTRESVIGYSEIRDRVDGLVGVVNGAYGLVG
                     QPAINYLHRSQSIEELVALYLAADVMLVTPVRDGMNLVCKEFIAARTDDTGVLVLSEF
                     TGAAESLGSALLINPYDTDGLKNAIDRAVHLPEADVRRRMELLRESVMTNDVYAWAHS
                     WMVALGIESEHDDAGAPS"
     gene            535..>3071
                     /gene="otsB"
                     /locus_tag="PKY13_15810"
     CDS             535..>3071
                     /gene="otsB"
                     /locus_tag="PKY13_15810"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOT81459.1"
                     /translation="MSWSTGLVEAIATAGRAPVLLIACDYDGTLAPIVDDPARAHPLR
                     PAIAGLRGLAALPDTHVAVVSGRSLRDLAVLSRLPEEVHLIGSHGSEFEPDSVGKLTA
                     DQVALLHRLSGEMAVIADAAPGCSIEIKPSGVAFHYRRAADPVAGEAAAKAILSSLGA
                     YQGVHVREGKCVVELSVVDTDKGGALDRLRAMVSADTVVFIGDDVTDEDGFARLRGPD
                     LGVKVGDGATAAAHRVADPEEVAGLLGRLLDERAAWVAGHRAPAIERHSLLSDQRTLA
                     LVDPEARISWMCHPRADSQAIFAELLGGPTAGYFAVKPLSNGGAPIQRYVSESMVVET
                     LWRTHKVTDYLDVSDGRPDESAGRTELVRVVEGNGPVEITFAPRLDFGRNVTELETVV
                     ADGVVIGVAVVGGHEPITLVAPGVEWEIVDAGTHQSARAVVHVGDDPVILDLRLGTDS
                     VSGDEPDRPVDEHHEAARRAENVAFWRAITGGLHLPPVATALVRRSVILLEALRYRPS
                     GAMLAAATTSLPESIGGLRNWDYRYCWPRDASMSCASLARLGRVEPGLALLDWLCDRI
                     DHATRPDLLRPLYRVTGDEHVPEGTIAELAGYAGSRPVRIGNAAENQVQLDALGPVAE
                     LVHLVFTSSHTMRPRHWALMEKIADVVVDHWHEPDHGIWEVRLARQHNVVSKVMCWQV
                     LDRAIALATATGREVPERWTESRSAIHAEVLERGWNADRNTFVSHYEGDHLDAGLLLM
                     GTCGFIDPSDERFDATVRRIERELCDGPAVYRYHDDDGLSGIEGGMLICTGWLVQALV
                     ACGRVDEAADWFDRLVLPAGPTGLLPEQIDPATERGLGNVPQAYSHLA"
BASE COUNT          429 a         1087 c         1075 g          480 t
ORIGIN      
        1 cctcgaggta ccgcgccacg tgatggtgca agtggccgag cccacccgcg agtcggtcat
       61 cgggtacagc gagatccgcg accgcgtcga cggtctcgtc ggtgtggtga acggcgcgta
      121 cggtctcgtg ggtcagccgg cgatcaacta cctgcaccgc agccagtcga tcgaggaact
      181 ggtcgcgttg tacctggccg ccgatgtgat gctggtgacg cccgtgcgcg acggcatgaa
      241 cctggtgtgc aaggagttca tcgccgcccg caccgacgac accggggtgc tggtgctgtc
      301 ggagttcacc ggcgccgccg agtcgttggg ctcggcgctc ctcatcaatc cctacgacac
      361 cgacggactc aagaatgcga tcgaccgagc tgttcatctc cccgaggccg acgtccggcg
      421 tcgtatggaa ctcctccggg agtcggtgat gaccaacgac gtgtacgcgt gggcgcactc
      481 gtggatggtc gcgctcggca tcgagagcga gcacgacgac gcaggagcac cgtcgtgagc
      541 tggtcgaccg gcctcgtcga ggcgatcgcc actgccgggc gggccccggt gctgctcatc
      601 gcctgcgact acgacggcac cctggcgccg atcgtggacg atccggcccg agcccatccc
      661 ctccgtccgg ccatcgccgg cctccgcggg ttggcggccc tgcccgacac ccatgtcgcc
      721 gtggtgtcgg gtcggtccct acgcgatctc gcggtgctgt cccggctgcc cgaggaagtc
      781 cacctcatcg gcagccatgg ttcggagttc gagcccgaca gcgtcggcaa gctcaccgcc
      841 gatcaggtgg cgctgttgca ccgactctcc ggcgagatgg ccgtcatcgc cgatgccgcc
      901 ccggggtgct cgatcgagat caagccgtcg ggcgtggcgt tccactaccg gcgcgccgcc
      961 gatcccgtgg ccggcgaggc cgctgcaaag gcgattctct cgagcctcgg cgcctaccag
     1021 ggcgtccacg tgcgcgaggg caagtgcgtc gtcgagctct ccgtcgtcga cacggacaag
     1081 ggcggtgcgc tcgaccgcct ccgggccatg gtgagcgccg acacggtcgt gttcatcggc
     1141 gacgacgtca ccgacgagga cggtttcgcc cgcctgcgcg gtcccgatct cggggtgaag
     1201 gtgggggacg gcgcgacggc ggcagcccac cgcgtggccg accccgagga ggtcgccggt
     1261 ctcctgggtc gtctgctcga cgagcgcgcc gcgtgggtcg ccggccaccg cgccccggcc
     1321 atcgagcgcc actcgctgtt gtcggatcag cgcaccctcg cgttggtcga ccccgaggcc
     1381 cgcatctcgt ggatgtgcca cccccgcgcc gacagccagg cgatcttcgc cgagctgttg
     1441 ggcggcccga ccgccggcta tttcgcggtg aagcctctct cgaacggcgg ggcgcccatc
     1501 cagcggtacg tgtcggagtc gatggtcgtg gagaccctgt ggcggaccca caaggtgacc
     1561 gactatctcg acgtgtccga cggtcggccc gacgagtcgg ccggtcgcac cgagctggtg
     1621 cgcgtcgtcg agggcaacgg ccctgtcgag atcaccttcg ccccgaggct cgacttcggc
     1681 cgcaacgtca ccgaactcga gaccgtcgtg gccgatgggg tggtcatcgg cgtcgcggtc
     1741 gtcggcggcc acgagccgat caccctcgtc gcccccgggg tcgagtggga gatcgtcgat
     1801 gcgggcacac accagagcgc ccgcgccgtg gtgcacgttg gcgacgaccc ggtcatcctc
     1861 gacctccgcc tcggcaccga ctcggtgtcg ggcgacgaac cggatcgccc cgtcgacgag
     1921 caccacgagg cggcccgtcg ggccgagaac gtggcgttct ggcgggccat caccggtggt
     1981 ctgcaccttc ctccggtggc gacggcactc gtccgccgct cggtgatcct gctcgaggcg
     2041 ctgcgttacc ggccctcggg ggcgatgctg gcagcggcga ccacgagcct gcccgagtcg
     2101 atcggcggcc ttcgcaactg ggactaccgc tactgctggc cgcgcgacgc gtcgatgtcg
     2161 tgcgcgtcgc tggcgcggct cggccgtgtc gaacccggtc tggcactgct cgactggctc
     2221 tgcgaccgca tcgaccatgc gacgcggccc gacctgctgc gaccgctcta ccgcgtgacc
     2281 ggcgacgagc acgttccgga gggcacgatc gccgagctgg cgggctacgc cggcagccgc
     2341 cccgtccgca tcggcaacgc ggcggagaac caggtgcagc tcgacgcgct cggaccggtc
     2401 gcggagttgg tgcacctcgt gttcacgagc agccacacca tgcgtccccg tcattgggcg
     2461 ctcatggaga agatcgccga cgtcgtcgtc gaccactggc acgaacccga tcacggcatc
     2521 tgggaggtgc gcctggcccg gcagcacaac gtcgtgtcca aggtcatgtg ctggcaggta
     2581 ctcgaccgtg ccatcgcctt ggcgacggcg accggacgcg aggtgccgga gcgctggacg
     2641 gagtcgcgtt ccgcgatcca cgccgaggtg ctcgagcggg gttggaacgc cgaccgcaac
     2701 acgttcgtgt cgcactacga gggcgatcac ctcgatgcgg gcctgctcct gatggggacg
     2761 tgcgggttca tcgatccgtc cgacgaacgg ttcgacgcga cggtgcgccg tatcgagcgc
     2821 gagctgtgcg acggtccggc ggtgtaccgt tatcacgacg acgacggact ctccggcatc
     2881 gagggcggca tgctgatctg caccggctgg ctcgtgcagg cgctcgtggc gtgcggccgc
     2941 gtcgacgagg cggcggactg gttcgaccgt ctggtgctcc ccgccggccc gacgggcctc
     3001 ctgcccgagc agatcgatcc ggcgaccgag cgcgggctcg gcaacgtgcc ccaggcctac
     3061 tcgcacctcg c
//
LOCUS       DAOSOA010000365         3061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32000_length_3061_cov_5.080838, whole genome shotgun sequence.
ACCESSION   DAOSOA010000365 DAOSOA010000000
VERSION     DAOSOA010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32000_length_3061_cov_5.080838"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /gene="pyrH"
                     /locus_tag="PKY13_15815"
     CDS             complement(<1..263)
                     /gene="pyrH"
                     /locus_tag="PKY13_15815"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378125.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT81460.1"
                     /translation="MTDGTAPDKPAARWPRVLLKLSGEAFAGDQGFGIDGEIVAQLAQ
                     SIIDVKAEMEVDIAVVVGGGNIWRGMTGAGAGMDRAQADYMGML"
     gene            complement(271..1062)
                     /gene="tsf"
                     /locus_tag="PKY13_15820"
     CDS             complement(271..1062)
                     /gene="tsf"
                     /locus_tag="PKY13_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT81461.1"
                     /translation="MADFTAQDVKALRDATGAGMMDAKKALVEAEGDAQRAARILREK
                     GLTKVATLEDRENNQGAVAIARDGNTAALVELKSETDFSAKSEDFVATVKKLAAAVLA
                     DGLGAIETLAEDIDTLKISKKENIVVGKVERFEAADGNVLDTYLHEQDGRGTVGVLLE
                     LAGADAELAHEISLHIAFAKPTGLSRDEIDPALVATAREGFEELTRKEGKPDQAIPKI
                     VEGRVNSWFAERILPEQGMFGDKETVQDRLGAGSVVRFALAVIGH"
     gene            complement(1171..2094)
                     /gene="rpsB"
                     /locus_tag="PKY13_15825"
     CDS             complement(1171..2094)
                     /gene="rpsB"
                     /locus_tag="PKY13_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOT81462.1"
                     /translation="MAAVVTMKQLIDAGVHFGHQTRRWNPKMQRFIHGEQNGIYIIDL
                     RITLQRIEKAYTYTRDLVADGGTILFIGTKRQAQDSVEGYATKCGMPYVNQRWLGGML
                     TNFETISKRIAKMKEYQRMRDSGEFESMPKKEALLLSRELVKLERNLGGMRHLEKAPN
                     AVFILDTKKEDIAVTEANKLGIPIVAVVDTNCDPDVIQYVIPGNDDAIRAGDLMARII
                     CDAVEEGRQIRARRTGAPVVMPRTVEEDAAKAQEQAEARRQAAAQAAQREARVAAAKE
                     EAAAVADEPAETPAAEAPADAVAADETPAAE"
     gene            complement(2273..2722)
                     /locus_tag="PKY13_15830"
     CDS             complement(2273..2722)
                     /locus_tag="PKY13_15830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT81463.1"
                     /translation="MFRKLATAIVVALLTRFDAPATPSYRPPLDRPVTSGFDESRGPY
                     GAGNRGLDYAVVPGDAVHTIGSGTVVFAGRVAGALWVTVLHPGGLRSSYGPMATIGVA
                     AGGAVDVGVVIGTVGSTFHLGVRRGETYVDPATLFEVRDRFAHLIPS"
     gene            complement(2799..>3061)
                     /locus_tag="PKY13_15835"
     CDS             complement(2799..>3061)
                     /locus_tag="PKY13_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT81464.1"
                     /translation="GDTIADSSHGPTGAFEVTEVRQMLSQAINGMPEREKVVLTLYYY
                     ESLTLSEIGRVLGVTESRVCQIHTKAMIQLRSRLAGLDRDPD"
BASE COUNT          432 a          993 c         1116 g          520 t
ORIGIN      
        1 agcatgccca tgtagtcggc ctgggcgcgg tccatgccgg caccggcgcc ggtcatgccc
       61 cgccagatgt tgccgccacc gacgaccacg gcgatgtcga cctccatctc ggccttgacg
      121 tcgatgatcg actgcgccaa ctgcgccacg atctcgccgt cgatgccgaa gccctggtca
      181 ccggcgaacg cctcgccgga gagcttgagc aggacccgcg gccaccgggc tgcgggcttg
      241 tcgggggccg taccgtcggt catcggcgac tcagtgaccg atgacggcga gggcgaatcg
      301 gacgaccgaa ccggcgccga ggcgatcctg cacggtctcc ttgtcgccga acatgccctg
      361 ctcggggagg atgcgctcgg cgaaccacga gttgacgcga ccctcgacga tcttcgggat
      421 cgcctggtcg ggcttgccct ccttgcgggt gagctcctcg aagccctccc gggcggtggc
      481 gacgagcgcc ggatcgatct cgtcgcggct gaggcccgtc ggcttggcga aggcgatgtg
      541 cagcgagatc tcgtgggcga gctcggcgtc ggcgccggcg agctcgagga gcaccccgac
      601 cgtgccgcgt ccgtcctgct cgtggaggta ggtgtcgagc acgttgccgt cggccgcctc
      661 gaagcgctcg accttgccca ccacgatgtt ctccttcttg gagatcttca gggtgtcgat
      721 gtcctcggcg agggtctcga tcgcgcccag gccgtcggcg agcaccgccg cggcgagctt
      781 cttgacggtg gcgacgaaat cctcggactt ggccgagaag tcggtctcgc tcttgagctc
      841 gaccagtgcg gccgtgttgc cgtcgcgggc gatggcgacc gcgccctggt tgttctcacg
      901 gtcctcgagg gtggcgacct tcgtgaggcc cttctcccgg aggatgcggg cggcgcgctg
      961 ggcgtcgccc tcggcttcga cgagcgcctt cttggcgtcc atcatcccgg cgccggtggc
     1021 gtcgcggagg gctttcacat cctgggcggt gaagtcggcc atgggggtac tcctggggtt
     1081 cgatcgtgga ggtgtcgcgc cggccgagcc ggtcgcgacg attgacgggg tgggtgaacc
     1141 ggcccgggcg atgccgcccg ggcaggtgaa ctactcggcg gcgggcgtct cgtcggcggc
     1201 cacggcgtcg gcgggtgcct cggcggcggg cgtctcggcg ggttcgtcgg cgaccgcagc
     1261 ggcctcttcc ttcgcagccg caacgcgggc ctcgcgctgc gcggcctggg cggcggcctg
     1321 acggcgggcc tcggcctgct cctgcgcctt cgcagcgtcc tcttcaacgg tgcgcggcat
     1381 cacgacgggt gcgccggtgc gacgggcccg gatctggcgg ccctcttcga cggcgtcgca
     1441 gatgatgcgg gccatgaggt cgccggcgcg gatcgcgtcg tcgttgcccg ggatgacgta
     1501 ctggatgacg tcgggatcgc agttggtgtc gacgacggcg acgatcggga tgccgagctt
     1561 gttggcctcg gtcaccgcga tgtcttcctt cttcgtgtcg aggatgaaca ccgcgttggg
     1621 cgccttctcg aggtgacgca tgccaccgag gttgcgctcg agcttcacga gctcacggct
     1681 caggaggagg gcttccttct tcggcatcga ctcgaactcg ccggagtcgc gcatgcgctg
     1741 gtactccttc atcttggcga tgcgcttcga gatcgtctcg aagttggtga gcatgccgcc
     1801 gagccagcgc tggttcacgt agggcatgcc gcacttggtg gcgtagccct cgacgctgtc
     1861 ctgcgcctgg cgcttggtgc cgatgaagag gatggtgcca ccgtcggcga ccaggtcgcg
     1921 ggtgtaggtg taggccttct cgatgcgctg cagcgtgatg cgcagatcga tgatgtagat
     1981 gccgttctgc tcaccgtgga tgaaccgttg catcttcggg ttccaacggc gggtctggtg
     2041 cccgaagtgg acccccgcgt cgatgagctg cttcatggtg acgactgcgg ccatgagttg
     2101 tacttccctt cggttgatgg cccgaggcgt cgcgcttcta ccgagagaaa cgaccgggga
     2161 cgcgccacgg gtgaggaatc agacaccgtg ggtcatggga ccgctcggcg gacgcggccg
     2221 atctggcacg tcggacggaa agtgtagtcg cgaacgcggc gtcttcccga actcagctcg
     2281 ggatcaggtg ggcgaagcgg tcccgcacct cgaacagggt ggccgggtcc acgtaggtct
     2341 cgccgcgacg gacgccgagg tggaacgtcg accccaccgt cccgatcacc acaccgacgt
     2401 cgaccgcgcc acccgctgcg acgccgatcg tcgccatcgg cccatacgag ctgcgcagcc
     2461 cgccggggtg gaggacggtg acccacaggg cgccggcgac gcgaccggcg aacacgacgg
     2521 tgcccgaacc gatggtgtgg accgcgtcgc cggggacgac ggcgtagtcg aggccacgat
     2581 tgccggcacc gtacgggcca cgggactcgt cgaacccgga agtcacgggc cggtcgaggg
     2641 gcggtcggta cgacggggtg gccggagcat cgaaacgggt caggagcgcg acgacgatgg
     2701 ccgtcgcgag cttgcggaac atgggggctc ccacggaacg gagcgcgaca ccagccgcga
     2761 ctcggaacgt tcaggggaag aacggggtgg agcctacgtc aatccggatc gcggtcgagc
     2821 ccggccaggc gggagcgcag ctggatcatg gccttggtgt ggatctggca cacccgcgat
     2881 tcggtgacac cgagcacgcg cccgatctcc gacagcgtca gcgactcgta gtagtagagc
     2941 gtgaggacga ccttctcgcg ctcgggcatg ccgttgatgg cctggctgag catctggcgc
     3001 acctcggtca cctcgaaggc gcccgtcggg ccgtggctgg agtcggcgat cgtgtcgccg
     3061 a
//
LOCUS       DAOSOA010000366         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32130_length_3052_cov_4.909576, whole genome shotgun sequence.
ACCESSION   DAOSOA010000366 DAOSOA010000000
VERSION     DAOSOA010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32130_length_3052_cov_4.909576"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1798
                     /locus_tag="PKY13_15840"
     CDS             269..1798
                     /locus_tag="PKY13_15840"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT81465.1"
                     /translation="MLAGCAPDIGRDISGVGAEYHPDVLPTGRMLDFPASESPIEHVV
                     VLMMENRSFDHYLGWLSRDEGYLERGLSRYGNTFAIDARSMQSYLDATSGQMVDTFHL
                     DPQSMANPWRGCGFNDPGHGWTAGHAQRDGGFLAPNSGNDRYALGYHEADDLPFHSRM
                     ARRFTTFDHYHASMLTSTQTNRRYLHSAQSGGYNYNYIPIAELGFQFDTIWDRLWRAG
                     VPVRSYFSDLPSLAFWGPRLGPLLSPIDQYFESCHAGNLPAVSFLDPPYAGPWQADDH
                     PHADPGAGQRFLRDVFQAFAESPCWENGLFLLTYDEWGGFFDHVAPPVLPDDRATNDD
                     ATNYGQAGFRVPTIMASPYAMPGFVDHRTYDHTSIMRFLQWRFLGAPAEGPGDDGSPW
                     ALKVRDRFANNIGNSLAFEQPDPTIFDIGDLPLRWPTAWCDGTAQLPAPGRPEAHPTA
                     TNVAPTILPGVLGPVPGEEQTEGGPGVDFADLLESGYYERAGLPTEPSSMARIWATPS
                     S"
     assembly_gap    1867..1966
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1967..2745)
                     /locus_tag="PKY13_15845"
     CDS             complement(<1967..2745)
                     /locus_tag="PKY13_15845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT81466.1"
                     /translation="MDAMDTSISPGRRTAIVTGAGRGIGAAIARALDSADHRVALVAR
                     SRDELDAVASTLTNDPVVVVADLGDADAPTAAVAEAIDAFVGRLDVLVNNAGMALRTP
                     SDQITAAEIDELFAVNVRSALLTSAAAIPTMVAAGSGSIVNISSISGLRGAPRRSVYA
                     ATKAALDGMTRALAMEFGPAGLRVNAVAPGVVETEMWRENLAKPGVADEVMAVIPTRR
                     LSTPEEVAAVVAFLASDSASAITGETISADGGIHATVNLWPT"
     gene            complement(2777..2980)
                     /locus_tag="PKY13_15850"
     CDS             complement(2777..2980)
                     /locus_tag="PKY13_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81467.1"
                     /translation="MHIEGSLLTERDAIQERRRRTKEYGYRVCGLWDGGVLEGRPRIC
                     VMPRGHESSPHRDADGEDLVDPS"
BASE COUNT          430 a         1056 c         1012 g          454 t
ORIGIN      
        1 gggcacgact acgaggtgtt ccccattccg ggcatctgag cgcccccgag cgttcggcgg
       61 cgaccgaggg tggcgatccc gcccgaaggg ctggtgggat ggcgcaggga ccggtaccgt
      121 cacgacgttc tggtgttcgg ctgacggcgg gacacgagcc ggccaccggc cggggagaca
      181 gcggggagac agcgatatgg ggatggatcg gcggcagttc ctacggcgct cgggaatgtt
      241 cgggggcgcc gcgttcttgg gtgaatcggt gttggcaggt tgcgctcccg acatcggtcg
      301 cgacatctcg ggggtcggag cggagtacca ccccgacgtg ctgccgaccg gccgcatgct
      361 cgacttcccg gcgtcggagt cgcccatcga acacgtcgtc gtgctgatga tggagaaccg
      421 ctccttcgat cactacctcg gatggctcag ccgcgacgag ggctacctcg agcgggggct
      481 ctctcgctac ggcaacacgt tcgccatcga cgccaggtcg atgcagagct acctcgatgc
      541 gacgagtggt cagatggtcg acacgttcca cctcgacccc cagtcgatgg cgaacccctg
      601 gcggggctgc ggcttcaacg acccgggcca cggctggacc gccggtcacg cgcaacgcga
      661 cggcgggttc ctcgcaccca acagtggcaa cgaccggtac gccctcggct accacgaggc
      721 cgacgacctg ccgttccact cccgcatggc ccgccggttc acgacgttcg accactacca
      781 cgcgtcgatg ctcacctcga cgcagacgaa ccgccgctac ctgcacagcg cccagtcggg
      841 tgggtacaac tacaactaca tcccgatcgc cgagctcggc ttccagttcg acacgatctg
      901 ggatcggctg tggcgcgccg gcgtgccggt tcgctcgtac ttcagcgacc tgccgagcct
      961 ggcgttctgg ggcccgcgcc tcggcccctt gctgtcgccg atcgaccagt acttcgagtc
     1021 gtgccacgcc ggcaacctcc ccgccgtctc cttcctcgac ccgccctatg ccgggccgtg
     1081 gcaggccgac gatcatccgc acgccgatcc cggtgccggc cagcggttcc tccgcgatgt
     1141 gttccaggcg ttcgccgaat cgccctgctg ggagaacggc ctgttcctcc tcacgtacga
     1201 cgagtggggt ggcttcttcg accacgtcgc gccgccggtg ctgcccgatg atcgggccac
     1261 gaacgacgac gccaccaact acgggcaggc cggattccgg gtgccgacga tcatggcgtc
     1321 gccgtacgcg atgcccggct tcgtcgacca ccgcacctac gaccacacgt cgatcatgcg
     1381 gttcctgcag tggcggttcc tcggtgcccc cgccgagggt cccggcgatg acggttcgcc
     1441 gtgggcactc aaggtgcgcg accgcttcgc caacaacatc ggcaactcgc tggcgttcga
     1501 gcagccggat ccgacgatct tcgacatcgg cgacctgccg ctccgctggc ccacggcgtg
     1561 gtgcgacggc accgcgcagc tcccggcgcc gggccgaccc gaggcccacc cgacggcgac
     1621 gaacgtcgct ccgacaatcc tgcccggcgt gttgggcccg gtccccggcg aggagcagac
     1681 cgagggcggc cccggcgtcg acttcgccga cctgctcgag tccgggtact acgagcgggc
     1741 cggcctgccg accgagccca gctcgatggc acgcatctgg gcgacgccga gctcctgagc
     1801 cgggtcagac cgtcggccag aggttgacgg tcgcgtggat gccgccgtcg gcggagatcg
     1861 tctcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtcg gccagaggtt
     1981 gacggtcgcg tggatgccgc cgtcggcgga gatcgtctcg ccggtgatcg cgctggcgga
     2041 gtcgctcgcg aggaacgcca cgaccgcggc gacctcctcg ggggtgctca gtcggcgggt
     2101 cgggatgacc gccatgacct cgtcggcgac gccgggcttg gcgaggttct cgcgccacat
     2161 ctcggtctcg acgacgccgg gtgccacggc gttgacccgc agcccggccg gaccgaattc
     2221 catcgcgagc gcccgggtca tgccgtcgag tgcagccttg gtcgccgcgt acacgctccg
     2281 gcggggcgcg ccccgcaggc cgctgatcga gctgatgttc acgatcgagc ccgagcccgc
     2341 cgccaccatc gtcgggatcg cggcagcgct cgtgagcaac gccgaccgga cgttgacggc
     2401 gaacagctcg tcgatctcgg ccgcggtgat ctggtcggac ggcgtgcgca gtgccatacc
     2461 ggcgttgttc accagcacgt cgaggcgacc aacgaacgcg tcgatcgcct cggccacggc
     2521 agccgtgggt gcgtcggcat caccgagatc agcgacgacg acgaccgggt cgttggtcag
     2581 cgtcgatgca acggcgtcga gctcgtcacg gctgcgggca acgagcgcga cccgatggtc
     2641 ggcggaatcg agggctcgtg cgatggccgc gccgatgccg cgaccggctc ccgtgacgat
     2701 cgcggtgcgt cgacccggcg agattgaggt gtccatcgcg tccatcgccg gagcctacga
     2761 cgctcacgga gctcggtcag gaggggtcga cgaggtcctc gccatcggcg tcgcggtgcg
     2821 ggctggactc gtgaccgcga ggcatgacgc agatgcgcgg acgaccttcg agcacgccgc
     2881 cgtcccacaa gccgcacacg cggtagccgt actccttggt ccgccgccgc cgctcctgga
     2941 tcgcgtcccg ctccgtgagc aggctcccct cgatgtgcac acgcggagcc tgcccggtgc
     3001 gggcgaccgc cggttggcgc gcaggtgtcc gctgggtgaa ttcacctcag gc
//
LOCUS       DAOSOA010000367         3050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32160_length_3050_cov_6.682805, whole genome shotgun sequence.
ACCESSION   DAOSOA010000367 DAOSOA010000000
VERSION     DAOSOA010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32160_length_3050_cov_6.682805"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PKY13_15855"
     CDS             <1..812
                     /locus_tag="PKY13_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81468.1"
                     /translation="QVRPLHRPPRTPADRQPHCAWTVIIDDEYPELPIPAAATAMGAT
                     RAANVELATPDPTEPGLGDYSGPLLDDLRFGDWSRSALLRIAEEVSLQWHLLALSFLS
                     AVRSRLPSADDAIGISRRQFTGIAGLTAERLRNLFDLGDDVVALASVIGLHPAFKPAA
                     YVDLSVEPEADRLVVTVGRGSDAVIEGAWPALLDVDHLDALDAIARAVNPRFVCEATG
                     STGEDLVVTFTMGDTDRPEADDVALTRFSTGADFAFATRGVPVAIRPTSGA"
     gene            complement(828..1430)
                     /locus_tag="PKY13_15860"
     CDS             complement(828..1430)
                     /locus_tag="PKY13_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT81469.1"
                     /translation="MTDTTIAADTAPQRATFHSMDQGTQEDWNLIATEAIHNFVGLPE
                     RLMAHLRLLDGDFGGFAVDRLTHSLQTAARAERAGRDDEYVMCALIHDIGDTLGPANH
                     ADIGAAILKPFISEQNHWMMEQHGIFQGYYFFHFLGADRNLRDQFEGHEWFDYCAEFC
                     HEYDQAAFDPAYDTPPLEHYEPLIRSICASPKRSIYKAVE"
     assembly_gap    1446..1545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1546..1974
                     /locus_tag="PKY13_15865"
     CDS             <1546..1974
                     /locus_tag="PKY13_15865"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HOT81470.1"
                     /translation="GLIKHVADTESGWIDLVLQLPAGGNDRYLENFTMRSDETLADVI
                     AAYDAVAARTTEVIDGIADLGQEVPVPKGVPWYPDDVDAWSVRWVLLHLIEETARHNG
                     HADIVREHVDGATAFPLMAAAEGWPATPWMQPWTPTSVPA"
     gene            2100..2759
                     /locus_tag="PKY13_15870"
     CDS             2100..2759
                     /locus_tag="PKY13_15870"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HOT81471.1"
                     /translation="MLGRRTTAVLAALVVVATLVTGCQPARAGARCRTTDWGDDGRDW
                     VLQCRNGRWVRALTKQDAAKILLAARPAAATPPTDAAGNPYPNDDYPAQWRNVAQDSV
                     FDSWGYPNRQCTSFVAWRLANTNRATLPVRPGDAGQWDDALRSHVVVDERPAVGAIAQ
                     WNENESFGGLGAGPIGHVAWVQAVHADGSVTVEQYNLGSDGTYVQFRTRAPRYIHVKD
                     L"
     gene            complement(2781..>3050)
                     /locus_tag="PKY13_15875"
     CDS             complement(2781..>3050)
                     /locus_tag="PKY13_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT81472.1"
                     /translation="LPEPLFGIAANRSIGLVIAGDDGNHVMRYAGFGQSCSPTSFGHA
                     GMHMQVGWADPASGISFAYLTNGADSDVMKEGGRGVALSDLAARL"
BASE COUNT          432 a         1031 c         1048 g          439 t
ORIGIN      
        1 cccaggtccg cccactccac cgaccgccac gcacgcctgc ggaccgtcag ccgcactgcg
       61 cgtggaccgt catcatcgac gacgagtacc ccgagctgcc gatccctgcg gcggcgacgg
      121 ccatgggggc aacccgcgct gcgaacgtcg agctggccac ccccgatccg accgagccgg
      181 gcctcggcga ctacagcggt ccgctgctcg acgacctgcg cttcggcgac tggtctcgct
      241 cggctctgct gcgcatcgcc gaggaggtga gcctgcaatg gcacctgctg gcgctgtcgt
      301 tcctcagcgc ggtgcgctcg cgcctcccgt cggccgacga tgcgatcggc atctcccggc
      361 ggcagttcac cggcatcgcc ggcctgaccg ccgagcggct ccggaacctg ttcgacctgg
      421 gcgatgacgt cgtcgcgctc gcgtcggtga tcgggctcca tccggcgttc aagccggcgg
      481 cgtacgtcga cctgtccgtc gagcccgagg ccgatcggct cgtggtgacc gtcgggcggg
      541 ggagcgacgc cgtgatcgag ggggcgtggc ccgccctgct cgacgtcgac cacctcgacg
      601 cgctcgatgc catcgcccgg gcggtcaacc cgcggttcgt gtgcgaggcg accggttcga
      661 ccggcgagga cctcgtcgtg accttcacga tgggcgacac cgatcgaccc gaggcggacg
      721 acgtcgcgct cacccggttc tcgaccggtg ccgacttcgc gttcgccacc aggggagtgc
      781 cggtcgcgat ccgcccgacg tcgggcgcct gacgctcgac tgcggcgcta ctcgaccgcc
      841 ttgtagatgc tccgcttcgg tgaggcacag atcgagcgga tgagtggctc gtagtgctcg
      901 agcgggggag tgtcgtacgc ggggtcgaag gccgcctggt cgtattcgtg gcagaactcg
      961 gcgcagtagt cgaaccactc gtggccctcg aactggtcgc gcaggttccg gtcggcgccg
     1021 aggaagtgga agaagtagta gccctggaag atgccgtgct gctccatcat ccagtggttc
     1081 tgctcggaga tgaacggctt gaggatggcc gcgccgatgt cggcatggtt ggccggaccc
     1141 agcgtgtcgc cgatgtcgtg gatcagcgcg cacatgacgt actcgtcgtc gcggccggct
     1201 cgctcggcgc gtgccgccgt ctgcagtgag tgggtgaggc ggtcgaccgc gaatccgccg
     1261 aagtcgccgt cgaggagccg aaggtgggcc atgaggcgct ccggaaggcc gacgaagttg
     1321 tggatcgcct cggtcgcgat gagattccag tcctcctggg tgccctggtc catggagtgg
     1381 aacgttgctc gttgcggtgc ggtgtccgcc gcgatcgtcg tgtcggtcat gaggccccct
     1441 gtggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcct catcaagcac
     1561 gttgccgaca ccgagtcggg ttggatcgac ctcgtgttgc agctcccggc cggcggcaac
     1621 gatcggtacc tggagaactt caccatgcgc tccgacgaga cgctcgccga cgtgatcgcc
     1681 gcctatgacg ccgtggccgc ccgcacgacc gaggtgatcg acggcattgc cgacctgggc
     1741 caggaggtgc cggtcccgaa gggtgtgccc tggtaccccg acgacgtcga cgcctggtcg
     1801 gtccggtggg tgctgttgca cctcatcgag gagacggcgc gccacaacgg ccacgccgac
     1861 atcgtccgcg agcacgtcga cggcgccacg gccttcccgc tgatggccgc cgccgaaggc
     1921 tggccggcca ccccgtggat gcaaccctgg acaccgacgt cggttcccgc ctgacccgac
     1981 ctgcgctgac cggtcccgcg gtgacctgac ccgcccgtcc ccacaggttc tgtcccgcga
     2041 acgagcgcga atcgcgccat cctcgcggga cagaaccggt ggccgatagc gtggccgaca
     2101 tgctgggacg cagaacgacg gcggtgctgg ccgcgctcgt ggtggtggcg acgctggtca
     2161 ccggctgcca accggcacgg gccggggctc gatgccgcac caccgactgg ggcgacgacg
     2221 gccgcgactg ggtcctgcag tgccgcaacg gacgatgggt gcgggcgctc accaagcagg
     2281 acgccgccaa gatcctgctg gcggcccggc cggcggcagc gaccccgccc accgacgcgg
     2341 ccggcaaccc gtaccccaac gacgactacc cggcccagtg gcgcaacgtc gcccaggact
     2401 cggtgttcga cagctggggg tacccgaacc gtcagtgcac cagcttcgtc gcctggcggc
     2461 tcgcgaacac caaccgggcg acgctccccg tgcgcccggg cgacgcgggg cagtgggacg
     2521 acgcgctgcg gtcccacgtc gtcgtcgacg aacgtccggc cgtcggggcc atcgcgcagt
     2581 ggaacgagaa cgagtcgttc ggcggcctcg gcgcgggacc gatcggccac gtggcctggg
     2641 tccaggccgt gcacgccgac ggcagcgtga ccgtcgagca gtacaacctc ggatccgacg
     2701 ggacgtacgt gcagttccgg acgcgggctc cccgctacat ccacgtgaag gatctctgag
     2761 gtcccgaacc gggagaaccg tcagagccgg gcggcgaggt cggacagcgc gacgccgcgg
     2821 ccgccctcct tcatcacgtc gctgtccgcg ccgttggtga ggtacgcgaa cgagatgccc
     2881 gacgccgggt cggcccagcc gacctgcatg tgcatgccgg cgtggccgaa cgaggtgggg
     2941 gagcatgatt ggccgaaccc ggcgtagcgc atgacgtgat tgccgtcgtc gccggcgatc
     3001 acgaggccga tcgagcggtt ggcggcgatg ccgaacagcg gctcgggcag
//
LOCUS       DAOSOA010000368         3043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32273_length_3043_cov_4.327309, whole genome shotgun sequence.
ACCESSION   DAOSOA010000368 DAOSOA010000000
VERSION     DAOSOA010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32273_length_3043_cov_4.327309"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..806)
                     /locus_tag="PKY13_15880"
     CDS             complement(129..806)
                     /locus_tag="PKY13_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81473.1"
                     /translation="MTMVEYVLFAGCLVMVAITAVGGLNQAARTYFQQSSAKIGQPVN
                     QITYGPDGSRQTQASTTTITAPPPSTTTTAAPTTTRATTTTVAPTTTRATTTVVSNPA
                     FVTTVSNTSATSQWWNGSANNGNGAWVANATFTNAYNRGQTLDLLVTRTMSDGSTTTS
                     TSTLYVGANGSATFSLYDNTLSKNSGVRTGVVSVTIAVTKVTTANVNWNTITSPGYGT
                     TTTINSI"
     gene            943..1554
                     /locus_tag="PKY13_15885"
     CDS             943..1554
                     /locus_tag="PKY13_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HOT81474.1"
                     /translation="MAGFTRGFQGRGHEERDPRLPPGQYDAGTTWPVLTAEATPRMSH
                     DQWTFTIEGLVDREVTWTWDEIHELPQSTYDGAIHCVTTWSKFDMHWEGVSVDTLLEA
                     AGPSPAATHVMAFTHSGYTTNLPLEDVVGGKAWVAFAADGEPLTPDHGGPARLLVPHL
                     YFWKSAKFVAGLRLLDHNAPGFWERNGYHDHGDPWREERYQGD"
     gene            1579..2337
                     /locus_tag="PKY13_15890"
     CDS             1579..2337
                     /locus_tag="PKY13_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HOT81475.1"
                     /translation="MTTVTEIRDETARAKTYRLRFPAPVPFRPGQHVIIRLTAPDGYT
                     ASRAYSIASAPDPQRPDELEVTVELLEGGEVSGFLHEEVVVGDELEVRGPIGGWFVWD
                     GTSPALLLGGGSGVVPLMSMLRTARATASTDRLRMIVSVRSADDLLYATELVGTELAG
                     TEPAGTELAGTDPDPTVRVVYTRSAPEGSDRRAGRLTVEDLRAVLPPDAGVTPIAYVC
                     GSAGFADSATRLLEEVGHPADRIRIERYGPSATA"
     gene            complement(2376..2870)
                     /locus_tag="PKY13_15895"
     CDS             complement(2376..2870)
                     /locus_tag="PKY13_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81476.1"
                     /translation="MGRTPRLATALVAAAVLAVAGCGGGSSAAAPTTAAPTTSHEMAG
                     MTTGDHSMDMAPTKAATNAGFGDCAQHMPGELLTPEEAVVRFDTQMVCLGYVTVTTGT
                     PIAWTNNDTAEQTVTILDDEDRELNEFTVDAGETITTPAASTGVYRFRITAIDSFTGT
                     IEVQ"
BASE COUNT          436 a         1000 c         1093 g          514 t
ORIGIN      
        1 cacccgcaac cgcggtacgg gcagggggag tgggggtggg cgccgtcatt ggagatacgg
       61 acgcacgaga gcgcgattcc gtttcgcggc gccgtccatc acctcatctt cgcctgcagc
      121 gcaggggtct agatcgagtt gatggtcgtc gtggtgccgt aaccggggct ggtgatggtg
      181 ttccagttga cgttggccgt cgtcaccttc gtgaccgcga tcgtcaccga caccacaccg
      241 gtgcgcacgc cggagttctt cgacagcgtg ttgtcgtaga gcgagaacgt cgccgacccg
      301 ttcgccccga cgtacagggt cgaggtggag gtggtggtcg acccgtccga catcgtccgg
      361 gtcacgagca ggtcgagggt ttgccctcgg ttgtaggcgt tcgtgaacgt ggcgttcgcg
      421 acccaggcgc cgttgccgtt gttggccgag ccgttccacc actggctcgt ggccgacgtg
      481 ttcgacacgg tggtgacgaa cgccgggttg ctcacaaccg tggtcgtagc ccgggtcgtc
      541 gtcggtgcga ccgtcgtggt cgtggcgcgg gtcgtcgtcg gcgcggccgt ggtcgtggtg
      601 ctcggcgggg gagcggtgat ggtcgtggtg ctcgcctgtg tctgacggct cccgtcgggc
      661 ccgtaggtga tctggttgac gggctggccg atcttggccg atgactgttg gaagtacgtc
      721 cgggccgctt ggttcaaccc gccgacggcg gtgatcgcga ccatcacgag gcacccggcg
      781 aagaggacgt actccaccat ggtcactccc gattccgacg ggtgtccgcg tcggcgacgt
      841 ctgctcacct cctgggcatt ggcatgcgga gggcgctccc gaaggagata cgacagtctt
      901 gaattatctt gagtgggggg tggcagggcc tagcatcggc cgatggcagg cttcacacga
      961 gggttccagg gccgcggcca cgaggagcgc gatccccggc tcccgcccgg ccagtacgac
     1021 gccggcacca cctggccggt cctgaccgcc gaggcgacac ctcggatgtc ccacgaccag
     1081 tggacattca ccatcgaggg cctcgtcgat cgcgaggtca cctggacgtg ggacgagatc
     1141 cacgagctgc cccagtcgac ctacgacggg gccatccact gtgtgacgac ttggtcgaaa
     1201 ttcgacatgc actgggaggg cgtctcggtc gacacgttgc tcgaagcggc aggcccctcg
     1261 cccgcggcga cccatgtcat ggcgttcacc cacagcggct acacgaccaa ccttcccctc
     1321 gaggacgtcg tcgggggcaa ggcgtgggtg gcgttcgccg ccgacggtga gcccttgacg
     1381 cccgaccacg gtggccccgc ccgcctgctg gtgccgcacc tgtacttctg gaagagcgcc
     1441 aagttcgttg ccggcctgcg gctgctcgac cacaacgctc ctggcttctg ggagcggaac
     1501 ggctaccacg atcatggcga tccgtggcgc gaagagcgct accagggcga ctgatcgatg
     1561 tcgggtgggc cctggtcgat gacgaccgtg accgagatcc gcgacgagac ggcgagagcg
     1621 aagacctacc ggctgcggtt ccccgctccg gtcccgttcc gtcccggcca gcacgtcatc
     1681 atccggctca cggcaccgga cgggtacacg gcgtcgcggg cgtactcgat cgcctccgca
     1741 cccgatccgc agcgccccga cgagctcgag gtcaccgtcg agctgctcga gggtggtgag
     1801 gtgtcgggct tcctgcacga ggaggtcgtc gtcggcgacg aactcgaggt gcgcgggccg
     1861 atcggcggct ggttcgtgtg ggacggaacg tcgccggcgc tgctgctcgg cggaggttcg
     1921 ggcgtggtgc cgctcatgtc gatgctccgc accgcacggg ctaccgcctc gacggaccgg
     1981 ttgcggatga tcgtgtcggt ccgcagcgcc gacgacctgt tgtacgccac cgagctggtg
     2041 ggcaccgagc tggcgggcac cgagccggcg ggcaccgagc tggcgggcac cgatcccgac
     2101 ccgacggttc gggtcgtgta cacccgcagc gcgcccgagg gcagtgaccg tcgcgccggt
     2161 cgactcacgg tggaggacct gcgggcggtg ttgccacccg acgccggggt cacccccatc
     2221 gcgtacgtgt gcgggtcggc aggcttcgcc gactcggcga cgcgtctgct cgaggaagtc
     2281 ggacatccgg ccgaccgcat tcgcatcgaa cgctacggac cgtccgcaac ggcgtagggg
     2341 cgacgaagct cgccaccccc acgccgcggg agcccctact gcacctcgat cgttccggtg
     2401 aacgagtcga tggcggtgat gcggaaccgg tagaccccgg tcgaagcggc cggcgtcgtg
     2461 atcgtctcgc cggcgtcgac cgtgaactcg ttgagctcgc ggtcctcgtc gtcgaggatc
     2521 gtgacggtct gctcggccgt gtcgttgttc gtccaggcga tcggggtccc ggtcgtcacg
     2581 gtgacgtagc cgaggcacac catctgcgtg tcgaagcgga ccacggcctc ctcgggagtg
     2641 agcagctcgc cgggcatgtg ctgggcgcag tcgccgaacc cagcgttggt ggccgccttg
     2701 gtcggggcca tgtccatcga gtgatcgccg gtggtcatgc cggccatctc gtgggaggtg
     2761 gtcggagcgg cggtggtggg tgcggcagcg gacgaaccgc caccgcaccc tgccactgcc
     2821 agcaccgcag cggcgaccag ggcggtcgcc agccgtggag tgcgtcccat cacgccgccg
     2881 tcgggtcctg gacgaccacc gcggtggcca tgccgaacag gcggtccttc atctcgacgt
     2941 ggttcaggat gtggcagtgc cagacccagg tgccgaggtc ggtggcctgc accagcaccg
     3001 tgtagcgctc gccggggccg atcaggatcg tgtccgccca gta
//
LOCUS       DAOSOA010000369         3037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32361_length_3037_cov_5.798457, whole genome shotgun sequence.
ACCESSION   DAOSOA010000369 DAOSOA010000000
VERSION     DAOSOA010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32361_length_3037_cov_5.798457"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKY13_15900"
     CDS             complement(<1..325)
                     /locus_tag="PKY13_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81477.1"
                     /translation="MDTFIALSIVIVLWTSALVGSVIGRRHRYRAVALQRLGGGLGVA
                     LATVAAATSDHAGDGLLDLDRFGTGIAVFVTTMSVVVTTFAGRSLAADARADRFFVAA
                     NALTGS"
     gene            435..1610
                     /locus_tag="PKY13_15905"
     CDS             435..1610
                     /locus_tag="PKY13_15905"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004680.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOT81478.1"
                     /translation="MLRDASTGPDGRRRPVVGMVGGGQLARMTAGAAARLGVSLSVLD
                     PDPGCPAAVGASVVVADVDDLTALVAFGREVDVVTFDHERIPVELVEALEAEGVAVAP
                     GSASCRFAFDKADSRARLDELGFPVPRFEIVSDAAGIVSVGDRFGWPVVAKAARGGYD
                     GRGVSVVERGDEEAAAALLGPVIVVEELVDFDQELAIMVARRPNGDSVTYPPVSTVQS
                     EGMCVEVVCPAAVPAAVADAARRLAVALADAVELVGVMAIELFLVGDELLVNELAARP
                     HNTGHHSIEACVTSQFEQHLRAILDLPLGSTDLRAPAAAMRNVVGSPESHEPTERLAE
                     ALEVTGAHVHLYGKQPRAGRKLGHVTALGATVDEARSVAERAADRLNGTTSPVGERS"
     gene            1607..2107
                     /gene="purE"
                     /locus_tag="PKY13_15910"
     CDS             1607..2107
                     /gene="purE"
                     /locus_tag="PKY13_15910"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT81479.1"
                     /translation="MTGEPLVGVVMGSSSDLETMRPAADVLSEFGISTEIRVVSAHRT
                     PQAMMDYAASAASRGRRVIIAGAGGAAHLPGMLAAITDLPVIGVPVPLRHLDGMDSLL
                     SMVQMPAGVPVATVSIGGARNAALLAARILAVADPRLVPLLAADRERRRALAEEQDRI
                     VAGGSE"
     gene            2104..2925
                     /gene="fabI"
                     /locus_tag="PKY13_15915"
     CDS             2104..2925
                     /gene="fabI"
                     /locus_tag="PKY13_15915"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HOT81480.1"
                     /translation="MSLLDGKRLLITGVVTTDSIAFATAAAALSHGAEIILTSFDRDR
                     ERASEAASELGHPIEMLELDVTQPAQFERLTADLRDRWGRLDGVLHAVAFAPRAALAG
                     DFLAADAASVQQAFHTSAHSYASLAKVLAELAPPGEGSIVGLDFDAAGAWPVYNWMGV
                     CKAALEAVSRYVARDLGPSGIRSNLVAAGPLHTRAAGGIPDFELLAAAWEARSPMRWD
                     VRDPAPVADAVCFLLSDMARAITGEILHVDGGYHAMAAGLERPWNGGTTSPVAVV"
BASE COUNT          456 a         1046 c         1065 g          470 t
ORIGIN      
        1 tcgatccggt gagggcgttg gcggcgacga agaagcggtc ggcccgggcg tcggccgcca
       61 gtgatcgccc ggcgaacgtc gtgaccacga cggacatggt cgtgacgaac accgcgatgc
      121 cggttccgaa gcggtcgagg tcgagcaggc cgtccccggc gtgatcggag gtggccgctg
      181 cgacggtggc gagcgccacg ccgagaccgc ccccgagccg ttggagtgcc acggcgcggt
      241 accggtgacg ccggccgatc acggagccga cgagagcgga cgtccagagg acgatgacga
      301 tcgagagggc gatgaacgta tccatatacg tgtaatatga cacgcgaaac agacttcggc
      361 aatagtcgag caacgaaaag gcgacgaaag gcggtttgga acacatgaag tcgcaaacgt
      421 gtaacgtgag gcgcatgttg cgggatgcat ctacagggcc cgacggtcgg agacgaccgg
      481 tggtcggcat ggtcggaggc ggccagttgg cccgaatgac ggccggcgcc gctgctcggc
      541 tcggagtgtc gctctccgtc ctcgatcccg acccgggctg tccggcggcg gtcggggcct
      601 cggtcgtcgt cgccgacgtg gacgacctga cggcactcgt cgccttcggt cgagaggtcg
      661 acgtggtcac gttcgaccac gaacggatcc ccgtcgagct ggtcgaggcc ctggaggccg
      721 agggggtggc ggtcgctccc gggtctgctt cctgccggtt tgcgttcgac aaggccgact
      781 cccgggctcg cctcgacgaa ctcggcttcc ccgtgccccg cttcgagatc gtgagtgacg
      841 ccgccgggat cgtgagcgtc ggcgaccggt tcgggtggcc cgtcgtggcg aaggctgccc
      901 gcggcggcta cgacggacgc ggcgtcagcg tcgtcgaacg cggcgacgag gaggcggccg
      961 ccgctctcct cggtccggtg atcgtcgtcg aggaactggt cgacttcgat caggaactgg
     1021 ccatcatggt ggcccgtcgt cccaacggcg actcggtgac gtacccgccg gtgtcgaccg
     1081 tccagagcga gggcatgtgc gtcgaggtcg tctgtccggc ggcggtgccg gccgcggtcg
     1141 ccgacgcagc tcgtcgcctc gccgtcgcgc tcgcagacgc cgtcgaactc gtcggagtga
     1201 tggcgatcga actgttcctc gtcggagacg aactgctcgt caacgagctc gccgcccggc
     1261 cgcacaacac cggtcaccac agcatcgaag cgtgtgtcac ctcccagttc gaacaacacc
     1321 tgcgggcgat cctcgatctt ccgttgggca gcaccgacct gcgggcgccc gctgctgcga
     1381 tgcgcaacgt cgtcggttcg ccggagtcac acgaaccgac cgaacggctc gccgaggcgc
     1441 tcgaggtcac cggcgcccat gtccacctct acggcaagca gccgcgtgcc ggccgaaagc
     1501 tcggccacgt caccgcgctc ggcgcaacgg tcgacgaggc ccgatcggtc gccgaacgcg
     1561 cggccgatcg actgaacggc acgaccagcc cagtggggga gcgctcatga ccggggaacc
     1621 gctcgtcggc gtcgtgatgg gcagctcgtc ggatctcgag acgatgcggc cggctgcgga
     1681 cgtcctctcg gagttcggga tctcgacgga gatccgcgtc gtgtcggccc accggacccc
     1741 tcaggccatg atggactacg ccgcatcggc ggcgagccgg ggtcggcggg tcatcatcgc
     1801 cggagccggg ggcgccgccc acctgccggg catgttggca gcgatcaccg atctccccgt
     1861 gatcggtgtg ccggtgccgt tgcgccatct cgacggcatg gactcgctgc tctcgatggt
     1921 gcagatgccg gccggtgtgc ccgtcgctac ggtctcgatc ggcggcgccc gcaacgcggc
     1981 gctgcttgcc gcgcgaatcc tcgccgtcgc cgatcctcgc ctcgtcccgc tcctcgcagc
     2041 ggaccgggag cgacgtcgtg cactggccga ggaacaggac cggatcgtcg ccgggggttc
     2101 cgaatgagcc tcctcgacgg gaagcgactc ctgatcaccg gcgtggtgac caccgactcg
     2161 atcgcgttcg ccaccgcggc tgcggccctg tcccacgggg ccgagatcat cctcacctcc
     2221 ttcgatcgcg atcgagagcg ggcgagcgag gctgcgtccg agctcggaca cccgatcgag
     2281 atgctcgaac tcgacgtgac ccagccggcc cagttcgagc gcctcactgc cgaccttcgc
     2341 gaccgctggg gccgactcga tggtgtgctc cacgccgtcg cattcgcgcc ccgggcggcc
     2401 ctggccggtg acttcctggc cgcggacgca gcctcggtcc agcaggcctt ccacacgtca
     2461 gcgcactcct acgcctcgct cgcgaaggtc ctcgccgaac tcgcgcctcc gggcgaaggc
     2521 tcgatcgtcg ggctcgattt cgatgccgcc ggggcgtggc ccgtctacaa ctggatgggt
     2581 gtgtgcaagg ccgccctcga agcggtgagc cgctacgtcg ctcgcgacct cgggcccagc
     2641 gggatccgga gcaacctcgt cgccgcgggt ccgttgcaca cccgggccgc cggtgggatt
     2701 cccgacttcg aactgctcgc tgcggcgtgg gaggcacgct cgcccatgcg ctgggatgtc
     2761 cgcgaccccg caccggtcgc tgacgccgtc tgcttcctgc tctcggacat ggcacgagcc
     2821 atcaccggcg agatcctgca cgtcgacggc ggctaccacg ccatggcggc cggtctcgag
     2881 cggccgtgga acggcggcac gacgagcccg gtcgcggtcg tctgacaacg gcgctgggga
     2941 acatcggagt tatcattcgt gagtcgcgtc gtccggacgc gacatcagga gatcaccgaa
     3001 tggacgagtt ggaggggcgg tcgagctgga ccttcct
//
LOCUS       DAOSOA010000370         3037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32364_length_3037_cov_4.712609, whole genome shotgun sequence.
ACCESSION   DAOSOA010000370 DAOSOA010000000
VERSION     DAOSOA010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32364_length_3037_cov_4.712609"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1426)
                     /locus_tag="PKY13_15920"
     CDS             complement(125..1426)
                     /locus_tag="PKY13_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81481.1"
                     /translation="MTTTSRPRLVFAAFLAAAVTVGCSATVSDTTAPAPSATTPANPT
                     SDAARCRQLQAAASANPPVFAAATTWNQPAVCLGTHPDSARWAHNWFNYATVPGRTDP
                     AKRGAIDVAFDEYSTPVYDAADATGQAAVFITGWGHGDNLGAQRTIPWNPSWLPANGN
                     DLELLILDRTTGREWGLWGYQKYNWSSCLTLANLAAGYRPGVDLCAAMATVSRTPTGE
                     FSDYRTSSGASDGWGRGMGAIQGLALLPTLDEIEKGSINHALNMETYSTMFGPACTTA
                     QMATAAAGVDCGFAVAPATRIEWPDGATSLCGANSQPGTSAGRAMTVPEGMRFAIDLT
                     DAQIDAWLDARGYRGAKRNTARIFAVALRDYGWITSDTSCWGASVSVDGIANPRSAAR
                     WNALGIDQFGPTGDTLLDGLITSADQVRAIRPPTSAPVLNR"
     gene            complement(1622..2524)
                     /locus_tag="PKY13_15925"
     CDS             complement(1622..2524)
                     /locus_tag="PKY13_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81482.1"
                     /translation="MMRTRRAMTGLVAMMGGAALVAVAPAGAQPAPGTTAAATAADKI
                     TVTQTALAPITTCTTPDRSLSYTTYSDASVFRLRVRASSPLCSPITAMAAIYAMPTDG
                     SVWPQTLSQSESFTISGASQTDIVFTKDCTPAQFDVVTGATPQTISPLGERHGPLLIP
                     DVNTAYQDPGIACGPPTTQVGITTTVPGGVTTPPATSTTVANTAVQNTTTTAAVVAGV
                     STTPTTALPSVLDTVTNRADTTYSPAALAVTGVSSQALGLVGIGMFTIGMGLMLTSRR
                     RAVPAVRMITSSWSVDPNSPFGLD"
     gene            complement(2685..>3037)
                     /locus_tag="PKY13_15930"
     CDS             complement(2685..>3037)
                     /locus_tag="PKY13_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81483.1"
                     /translation="VNAWLVGYTWLQHTDVDVPHYADDEWSWLLGAFQTIDRPYGRLV
                     DALHHRIGSTHVAHHIDHRIPHYHAARATEAIAAAFPAWYRFDPTPVPVALWRVGREC
                     VAVDRTEDGWRFRP"
BASE COUNT          417 a          970 c         1191 g          459 t
ORIGIN      
        1 agatcagggt gtcgggcccc gtgctgcagg agaccgcggg cgactccgga ggtgccgaac
       61 aggaccgcgg cagcggcgac cgcgacgagc gcggtcacac ggggcgcgct cccgcggggc
      121 atcgtcagcg gttgagcacc ggcgccgacg tcggcggccg gatggcgcgg acctggtcgg
      181 cggaggtgat caggccgtcg agcagggtgt cgccggtcgg tccgaactgg tcgatgccga
      241 gcgcgttcca gcgggccgcc gaccgggggt tggcgatgcc gtcgaccgac accgaggcgc
      301 cccaacagct cgtatcggag gtgatccagc cgtagtcgcg gagggcgacg gcgaagatcc
      361 gcgccgtgtt gcgcttggcg ccgcggtacc cgcgggcgtc gagccaggcg tcgatctggg
      421 cgtcggtcag gtcgatggcg aagcgcatgc cctcaggcac ggtcatggcc cggccggccg
      481 acgtgccggg ctgggagttg gcgccgcaga gcgaggttgc cccgtcgggc cattcgatgc
      541 gcgtcgccgg ggcgaccgcg aagccgcagt ccacaccggc ggcggcggtc gccatctgcg
      601 ccgtcgtgca ggcgggaccg aacatggtcg agtacgtctc catgttgagg gcgtggttga
      661 tggagccctt ctcgatctcg tcgagggtgg gcaggagggc gaggccctgg atcgcgccca
      721 tgccgcgacc ccacccgtcg gaggcgccgc tcgatgtgcg gtagtcgctg aactcgccgg
      781 tcggggttcg gctcacggtg gccatggcgg cgcagaggtc gacccccggt cggtaaccgg
      841 cggcgagatt ggcgagggtc aggcaggacg accagttgta cttctggtag ccccacaggc
      901 cccactcgcg accggtggtg cggtcgagga tgaggagttc gaggtcgttg ccgttggcgg
      961 ggagccacga cgggttccac gggatggtcc gctgggcgcc caggttgtcg ccgtggcccc
     1021 acccggtgat gaagacggcg gcctgcccgg tcgcgtcggc cgcgtcgtag accggcgtgg
     1081 agtactcgtc gaacgcgacg tcgatcgcgc cgcgcttggc cgggtcggtg cggcccggga
     1141 cggtggcgta gttgaaccag ttgtgggccc agcgcgccga gtcgggatgc gtgccgaggc
     1201 acacggcggg ctggttccag gtcgtcgcgg cggcgaagac gggcgggttg gccgaggcgg
     1261 cggcctggag ctggcggcag cgggcggcgt cggaggtcgg gttcgccggc gtggtggccg
     1321 agggagcggg ggccgtcgtg tcggagacgg tggcggagca cccgaccgtc acggcggcgg
     1381 cgaggaatgc cgcgaagacg aggcgtggcc tcgaggtggt cgtcatcggg ttctccagcg
     1441 gtgaggggag cagttcactt ggtccacggc cgtcgatccc ccggcgggtc gtgggtactg
     1501 ctcctcggtt acctacccta tcggcgcaga tgacagcgcc gtaacagtcc ccgttcgggt
     1561 gaaattgttc aggggagggt gggcaccgct gcccaactgg gagtccgtcg aggccgaccc
     1621 gtcagtcgag gccgaacggg ctgttcggat cgaccgacca gctcgacgtg atcatccgca
     1681 ccgccggcac tgcccgtcgt cgcgacgtga gcatgagccc catcccgatc gtgaacatgc
     1741 cgatgcccac caggcccagg gcctgcgagg acacgccggt gaccgccagc gcggccgggc
     1801 tgtaggtcgt gtcggcgcgg ttcgtcaccg tgtcgaggac cgacggcagg gcggtcgtgg
     1861 gggtcgtcga gacaccggcg accacggcgg ccgtggtcgt ggtgttctgc accgcggtgt
     1921 tggccaccgt ggtggacgtg gcgggcggtg tggtgacccc gccgggaact gtcgtcgtga
     1981 tgccgacctg cgtggtcggc ggtccgcagg cgatgcccgg gtcctggtag gccgtgttca
     2041 cgtcggggat caggagcggt ccgtggcgct cacccaaggg agagatcgtc tggggcgtgg
     2101 cgccggtgac cacgtcgaac tgggcagggg tgcagtcctt ggtgaacacg atgtcggtct
     2161 ggctcgcccc gctgatcgtg aacgactcgg actgcgagag cgtctgtggc cacaccgagc
     2221 cgtcggtcgg catggcgtag atcgccgcca tggcggtgat cggcgagcac aagggcgagg
     2281 aggcccgcac ccggaggcgg aagaccgagg cgtcggagta cgtcgtgtag ctgagcgagc
     2341 ggtcgggcgt ggtgcacgtc gtgattggtg cgagggccgt ctgggtcacc gtgatcttgt
     2401 cggcggccgt cgctgcggcc gtcgtgccgg gcgccggttg ggctccggcc ggagcgaccg
     2461 cgacgagggc cgctccaccc atcatggcca cgagacccgt catcgcccgt cgtgttcgca
     2521 tcatgtccct cacatcgccg tccccaggtc cccgccggta cccatcgcac ttcggatcca
     2581 taccaggccg tccgcagcga tcggcatcat gtcacggttc cccgtggtgc cacatcccct
     2641 gaggtggaat tgcccgaccc cccgaacccc accggatcga acggtcaggg acggaaccgc
     2701 cagccgtcct cggtgcggtc gacggcgacg cactcccgtc ccacccgcca gagcgcgacg
     2761 ggcaccggcg tgggatcgaa ccggtaccac gccgggaacg cggcggcgat cgcctcggtc
     2821 gcccgggcgg cgtggtagtg gggaatccga tggtcgatgt gatgggcgac gtgggtgctg
     2881 ccgattcggt ggtggagcgc gtcgacgagg cggccgtacg ggcggtcgat cgtctggaac
     2941 gccccgagca gccagctcca ctcgtcgtcg gcgtagtggg gcacgtcgac gtcggtgtgc
     3001 tgcaaccacg tgtaccccac gagccaggcg ttcacca
//
LOCUS       DAOSOA010000371         3032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32427_length_3032_cov_5.369835, whole genome shotgun sequence.
ACCESSION   DAOSOA010000371 DAOSOA010000000
VERSION     DAOSOA010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32427_length_3032_cov_5.369835"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PKY13_15935"
     CDS             <1..868
                     /locus_tag="PKY13_15935"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOT81484.1"
                     /translation="PPRAGAVITNIEPDHLDHHGSFEELVAAFERFADGTEGPVIICA
                     DDVGAAPLIDQFPQSHSYGESGLADFVISDVDATDGMRWTLTDPDGVEHHLSLSLPGR
                     HLVLNATAAFALAVSIGADPVGAAAGIARYRGVGRRFEHRGTTAGITFIDDYAHMPTE
                     VRAVLDAATSGAAAGGWDRIVAVFQPHRYSRTGSVWREYADAFAAADVVVITDIYAAG
                     EAPVDGVTGHLVVDAVAAAHPHAEVLWLPTRADLLAGLPALLGTGDLCLTMGAGDLTT
                     LPDQLIAALEAE"
     gene            868..1833
                     /gene="murB"
                     /locus_tag="PKY13_15940"
     CDS             868..1833
                     /gene="murB"
                     /locus_tag="PKY13_15940"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOT81485.1"
                     /translation="MGEPDVAFAASLDDVGAALFDAGLDVEAQRPLGPLTTYRVGGRA
                     SWFAEVRSAEELVRLAEVTSSTGVRTLVVGKGSNLLVADSGFPGVAVQLGEAFAAVDV
                     DGTTVVAGGAASLPVVARRTVAAGLTGFEWAVGVPGSIGGAVRMNAGGHGSDMAASLR
                     EVRVVDLRAGQDVVMSVVECDLGYRRSSIGAHQIVVEATLDLADGNAEAGEAELAEIV
                     RWRRHHQPGGQNAGSVFTNPTGDSAGRLIDVAGCKGHRIGTAEVSTKHANFVQADPGG
                     RADDIVALMVHVRSTVAETLGVDLHAETAMVGYDAATVEAAGGRS"
     gene            1830..2729
                     /locus_tag="PKY13_15945"
     CDS             1830..2729
                     /locus_tag="PKY13_15945"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOT81486.1"
                     /translation="MSDTMRPGDTGQVDAVRAGRRGDADDRPQVLAYGPDELYPDPYA
                     PSMTGWSDEPEDTSFEDYVADPRVVATRAMDLAELALEVTARPDADPHDVVPQRAVID
                     IALDPPRYADDDPTAGPGVTDPFASSLVGDEPTSADPGIVVDERLERRREDVVSDNRR
                     RRRRRVGALFGLVVLGAGVVGAAYSPLLDVDDISIVGSDQLDLDGLAARTGVRIGDPI
                     LFVDPASVAAELRDDPRFVRVVVQRRFPDSISVRLTDRRAIAVVVGPTRGVVVGEGGV
                     IIAVETTPQTSANGLSRPQNEPV"
     gene            2753..>3032
                     /locus_tag="PKY13_15950"
     CDS             2753..>3032
                     /locus_tag="PKY13_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81487.1"
                     /translation="MERNALQQVAEGDAEDQRRHGAADEEAPVPRRAPARILDLAAVI
                     EADRAEEERPQHGEHRPVEAGEGRRINQRPGGEDGTAAGDEPHLVAVPV"
BASE COUNT          421 a         1091 c         1078 g          442 t
ORIGIN      
        1 gccgccccgc gccggcgccg tgatcaccaa catcgagccg gaccacctcg accaccacgg
       61 atcgttcgag gagctcgttg cggccttcga acgcttcgcc gacggcaccg agggtcccgt
      121 gatcatctgt gccgacgacg tcggcgccgc tccgctcatc gaccagttcc cccagtcgca
      181 ctcctacggc gagtccggcc tggccgactt cgtgatctcc gacgtcgacg ccaccgatgg
      241 catgcggtgg acgctcaccg atcccgatgg cgtggagcac cacctctcgt tgagcctgcc
      301 cggtcggcac ctcgtcctca acgccaccgc ggcgttcgcg ctcgcggtgt cgatcggcgc
      361 cgatcccgtc ggcgctgcgg cgggcatcgc tcgctatcgc ggcgtcggtc gccgcttcga
      421 gcaccgcggc accaccgccg gcatcacgtt catcgacgac tacgcccaca tgccgaccga
      481 ggtccgcgcc gtcctcgacg ctgccacgag tggtgccgcc gcgggcgggt gggatcgcat
      541 cgtcgccgtc ttccaacccc accgctacag ccggaccggt tcggtgtggc gggagtacgc
      601 cgatgcgttc gccgcggccg acgtcgtggt gatcaccgac atctacgcgg cgggggaggc
      661 ccccgtcgac ggcgtcaccg gccacctcgt ggtggacgcc gtcgccgcgg cgcatccgca
      721 cgccgaggtg ctgtggctcc cgacccgggc cgacctcctc gccgggctac cggcactgct
      781 cggcaccggc gacctctgcc tcacgatggg cgccggcgac ctgacgacgc tgcccgacca
      841 gctcatcgcg gccctggagg cggagtgatg ggcgagcccg acgtggcgtt cgccgcgtcg
      901 ctcgacgatg tgggcgcggc gctgttcgac gccggtctcg atgtcgaggc tcaacgtccg
      961 ctgggtccgc tcaccaccta ccgagtggga gggcgggcgt cgtggttcgc cgaggttcgg
     1021 tcggcggagg aactcgtccg gctcgcggag gtcacgagct cgacgggcgt gcggacgctg
     1081 gtggtcggca agggctccaa tctcctcgtc gccgactccg ggttcccggg cgtcgccgtg
     1141 cagctgggtg aggcgttcgc cgcggtcgac gtcgacggga ccaccgtcgt ggccggaggt
     1201 gcggcgagcc tgccggtcgt cgcccggcgc accgtggccg cgggcctcac cgggttcgag
     1261 tgggccgtgg gtgtgcccgg gagcatcggt ggcgccgtgc gcatgaacgc gggcggtcac
     1321 gggtcggaca tggcggcctc actgcgggag gtccgcgtgg tcgatctgcg cgccggtcaa
     1381 gatgtcgtga tgtccgtggt ggagtgcgac ctcgggtacc ggaggtcgtc gatcggtgcc
     1441 caccagatcg tcgtcgaggc gaccctcgac ctggccgacg gcaacgccga agccggtgag
     1501 gccgagttgg ccgagatcgt gcggtggcgt cgccaccacc agcccggcgg gcagaacgcc
     1561 gggtcggtct tcaccaatcc caccggcgac tcggccggtc ggctcatcga cgtggccggg
     1621 tgcaaggggc accggatcgg gaccgcggaa gtgtcgacga agcacgccaa cttcgtccag
     1681 gccgaccccg gtggccgcgc cgacgacatc gtggcgctca tggtccacgt ccggtccacc
     1741 gtcgccgaga cgctgggcgt cgacctgcac gccgagaccg ccatggtcgg gtacgacgcg
     1801 gcgaccgtcg aggcagccgg aggccggtcg tgagcgacac gatgcgcccc ggcgacacgg
     1861 gtcaggtcga cgcggttcgg gccggccgtc gcggcgacgc cgacgaccgt ccgcaggtgc
     1921 tcgcgtacgg ccccgacgag ctgtaccccg atccgtacgc gccgtcgatg acggggtgga
     1981 gcgacgagcc cgaggacacc agtttcgagg actacgtggc cgacccccgg gtcgtcgcga
     2041 cccgggcgat ggatctggcc gaactcgcgc tcgaggtgac cgcccgcccc gacgccgatc
     2101 cccacgacgt cgtgccccag cgggccgtga tcgacattgc cctcgacccg ccccggtacg
     2161 cggacgacga cccgacggcc ggtcccggcg tcaccgatcc gttcgcatcc tcgctcgtcg
     2221 gcgacgagcc gacgtcggcg gatccgggca tcgtcgtcga cgaacgactg gaacgccggc
     2281 gcgaggacgt cgtctcggac aaccgtcgcc gtcgccgtcg gcgcgtcggc gctctcttcg
     2341 gactggtggt gttgggtgcc ggcgtcgtcg gcgccgcgta ttcgccgttg ctcgacgtcg
     2401 acgacatctc gatcgtcggg tccgaccagc tcgacctcga cggtcttgcc gcccgcaccg
     2461 gcgtccgcat cggcgatccg atcctcttcg tggacccggc gtcggtcgcc gccgagctgc
     2521 gcgacgaccc tcggttcgtc cgcgtcgtcg tgcagcgccg ctttcccgac tccatctcgg
     2581 tgcggctcac cgaccgtcgg gccattgcgg tggtcgtcgg cccgacccgc ggtgtggtcg
     2641 tgggggaggg cggtgtgatc atcgccgtcg agacgacgcc gcagacgagc gcgaatgggt
     2701 tgagcaggcc ccagaacgag ccggtgtagt aggaaacgag attctcatcg aagtggaaag
     2761 gaacgccctg caacaggttg ccgaaggcga cgccgaagat cagcggcggc acggcgccgc
     2821 cgacgaagag gccccagtcc cacgtcgagc gccagcgcgc atcctggatc ttgctgcggt
     2881 aatcgaagcc gaccgggcgg aagaagagcg cccacagcac ggcgagcatc gcccagtaga
     2941 agccggagaa ggccgtcgca taaaccagcg gccaggcggc gaagatggca ccgccgccgg
     3001 tgatgaacca cacctggttg ccgtcccagt gc
//
LOCUS       DAOSOA010000372         3019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32593_length_3019_cov_4.808030, whole genome shotgun sequence.
ACCESSION   DAOSOA010000372 DAOSOA010000000
VERSION     DAOSOA010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32593_length_3019_cov_4.808030"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PKY13_15955"
     CDS             <1..1241
                     /locus_tag="PKY13_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81488.1"
                     /translation="IVLAATACGPDVPAPTGPTWITTHVSRTVVGTTTTNYVGAWTDL
                     DWASTLQVDTGAGGSGTASLLLFPRSGPGNSTLGSPQTLTPTVSPSLSGPIGGHVIGL
                     PGTVNGNPAIEFFKRTAGTWGSAGLFNLPANQQVAAMTDAWLVTRDVPINAGVEAHVY
                     LFPLDTSGPAVVVGSATTLGPDPAWPLALREGFGGPGAAIDGDLLVVGAQGQFSPTPG
                     GARVFRATGSVWAPVLSLGASPTGPNNYARAVAVDDGPSIDRIALGPQADGISSVEVF
                     ADSGSGFTLEQTIVRDPGDPDEFQGLLFGNGLAIDGDLLAVTARAVLVPSAQVGHADV
                     KVGYVQLFRKGATWAREAEVPVFTNPASPGTVSSLPFKLQAAGNHVAVFVFVTPDPPP
                     GCTFPCFNFGVEAWSIDRTG"
     gene            1238..2362
                     /locus_tag="PKY13_15960"
     CDS             1238..2362
                     /locus_tag="PKY13_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81489.1"
                     /translation="MSCDLAEVSTTTRSRRRRLVSAVGIALLVPLASCSGDDGTIAVG
                     DRSTTSAPSTTDAAAATTTPAADRFVSGSYLEETRPTPGEVTLTLHDPDGSAVRTVYS
                     APTYTVGDDLEASTILDAELAPDGGVFVVERTDRSPDAGLRPGGTRLAKVAADGTATT
                     VKANVVASELSPDGSHMAIVVLSPDGDGDGSGTQAIRVIDLATGSVDELWATDVPLDE
                     TGVITVEIGSTRVVGWIADESTLIVEESCCDSGAVMLLPSSGRSAAPETWPAVRGDHA
                     TYALGTDAEGRVLVQRTVFDGDGIEVPMSFAGIEVVALAADGTVSEPLYTERATEETE
                     DRVRFDLIGDQVPGTVLPIAAQGPPGLFGAGDERTPRRFT"
     gene            2444..>3019
                     /locus_tag="PKY13_15965"
     CDS             2444..>3019
                     /locus_tag="PKY13_15965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT81490.1"
                     /translation="MAPTTSALSPTDAPTISGPSAEAPMVATLHGTHTSSTRRTGSTR
                     YGPFELRWTETGPFDGPVTLLLHGIYAGAHGYEWRELVPLLDGSRPGAGAPRRVRVAD
                     LLGAGGSDRPDLEYDPTVVLGAVRALIGDCGPHCTVVASSLTGAYATKAIADSIRPGD
                     TQADTPRLVLVTPTGLGDAQRRRGGLVARAAY"
BASE COUNT          397 a         1158 c         1016 g          448 t
ORIGIN      
        1 cgatcgtgct ggcggcgacc gcgtgtggcc ccgacgtccc ggccccgacc ggcccgacgt
       61 ggatcacgac ccacgtctcg cgcaccgtcg tgggcaccac cacgacgaac tacgtgggcg
      121 cctggaccga tctcgactgg gcgtcgacac tccaggtcga caccggcgcc ggcggaagtg
      181 gcaccgcgag cctgctcctc ttcccacggt cgggcccggg caactccaca ctcggcagtc
      241 cacagaccct caccccgacg gtgtcaccgt ccctctcggg cccgatcggt ggtcatgtga
      301 tcggcttgcc gggaacggtc aacggcaacc ccgcgatcga gttcttcaag cgcaccgccg
      361 gcacgtgggg gtcggcgggt ctgttcaacc tgccggcgaa ccagcaggtc gccgccatga
      421 ccgacgcctg gctcgtcacc cgcgacgtcc cgatcaacgc cggcgtcgag gcccacgtct
      481 acctctttcc gctcgatacg tccggcccgg cggtcgtggt gggcagcgcg accaccctcg
      541 gtcccgatcc cgcctggccg ctggcgttgc gcgagggctt cggtgggccc ggtgcggcga
      601 tcgacggtga cctcttggtc gtgggggccc aggggcagtt cagcccgacg ccgggcggcg
      661 cccgcgtgtt ccgggcgacc ggttcggtgt gggcacccgt cctctccctc ggagcctcac
      721 ccaccggccc caacaactac gcccgtgccg tcgccgtcga cgacggcccg tcgatcgacc
      781 gcatcgctct cggcccccag gccgacggca tctcgtcggt cgaggtgttc gccgattcgg
      841 gctcggggtt cacgctcgaa cagaccatcg tgcgcgaccc cggcgacccc gacgagttcc
      901 aggggctcct cttcggcaac gggctcgcca tcgacggcga tctgttggct gtgacggcgc
      961 gcgccgtgct cgttccgtcg gcccaggtcg gccatgccga cgtcaaggtc ggctacgtgc
     1021 agctcttccg caagggggcg acctgggccc gtgaggccga ggtgcccgtg ttcaccaatc
     1081 cggcgtctcc cggcacggtg agctcgttgc ccttcaagct ccaggcggcc ggcaaccacg
     1141 tggcggtctt cgtgttcgtc acccccgacc cgccgcccgg ctgcaccttc ccctgcttca
     1201 acttcggcgt cgaggcctgg tcgatcgacc ggaccgggtg agctgcgacc tggccgaggt
     1261 ctcgacgacc actcgatcgc gtcgacggcg cctcgtgtcc gcggtcggca tcgcgctgct
     1321 cgtgccgctg gcgtcgtgct ccggcgacga tggcaccatc gccgtcggcg accggtcgac
     1381 cacgtcggca ccctcgacga ccgacgctgc agcggcgacg acaaccccgg cggccgaccg
     1441 gttcgtgtcc ggctcgtacc tcgaggagac ccgccccacc cccggcgagg tcaccctcac
     1501 gctccacgat cccgacggct cggcggtacg gaccgtctac tccgcaccga cctacaccgt
     1561 gggcgacgac ctcgaggcga gcaccatcct cgacgccgag ctggcacccg acggcggggt
     1621 cttcgtcgtc gagcggaccg accggtcgcc cgacgccggc ctccggcccg gcggcacccg
     1681 gctggcaaag gtcgccgccg acggaaccgc cacgaccgtg aaggcgaacg tcgtggccag
     1741 tgagctgtcg ccggacggca gccacatggc gatcgtggtg ttgtcgcccg atggcgacgg
     1801 cgacggctct ggcacccagg cgatccgggt catcgatctg gcgaccggca gcgtcgacga
     1861 actgtgggcc accgacgtgc cgctcgacga gaccggtgtg atcaccgtcg agatcggctc
     1921 cacccgcgtg gtcgggtgga tcgccgacga gtcgacgttg atcgtcgagg agagttgctg
     1981 cgattccggc gctgtgatgc tcctgccgtc gtcgggccgg tccgccgcgc ccgagacgtg
     2041 gccggccgtt cgtggcgacc acgccaccta cgccctgggc accgatgccg agggtcgggt
     2101 gctcgtgcag cgcacggtgt tcgacggcga cggcatcgag gtgccgatgt cgttcgccgg
     2161 catcgaggtc gtggcgcttg ctgccgacgg cacggtctcg gagccgctct acaccgagcg
     2221 tgccaccgag gagaccgagg atcgggtgcg gttcgacctc atcggcgacc aggtaccggg
     2281 cacggtcctc ccgatcgccg ctcagggccc gcccgggttg ttcggtgccg gcgacgagcg
     2341 gacaccccgc cgcttcacct gagaccaccg ctcattggtc gaaccgaacc cccgtttcga
     2401 gggaggtcgg tctggcgggg gagcgctcag ggaacgaggg tgcatggccc cgaccacctc
     2461 cgcactgtca cccactgatg cgccgacgat atccggcccc tcggccgagg ccccgatggt
     2521 cgccacgctc cacgggaccc acacgtcctc gacgcgccgg accggctcga cccgctacgg
     2581 ccccttcgaa ctccgctgga ccgagaccgg acccttcgac ggccccgtca ccctcctgct
     2641 gcacggcatc tacgccggtg cccacgggta cgagtggcgc gaactcgtgc ccctgctcga
     2701 cgggtcgagg cccggcgccg gtgcgccgcg ccgggtccgc gtcgccgacc tgctcggcgc
     2761 gggcgggagc gatcgccccg acctcgagta cgaccccacc gtcgtcctgg gcgccgtccg
     2821 tgcgctcatc ggtgactgcg ggccgcactg cacggtcgtc gccagttccc tcaccggggc
     2881 gtacgcgacg aaggcgatcg ccgattcgat ccgaccgggc gacactcaag cggacacccc
     2941 gcgcctcgtt ctggtcactc cgacgggcct cggcgacgcc cagcgccggc gcggcggcct
     3001 cgttgcccgg gcggcgtac
//
LOCUS       DAOSOA010000373         3017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32623_length_3017_cov_7.165429, whole genome shotgun sequence.
ACCESSION   DAOSOA010000373 DAOSOA010000000
VERSION     DAOSOA010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32623_length_3017_cov_7.165429"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..896
                     /locus_tag="PKY13_15970"
     CDS             120..896
                     /locus_tag="PKY13_15970"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOT81491.1"
                     /translation="MLLAIDVGNTQTVIGLYKSGDESHELLDHWRIATNAVRTSDELA
                     LMFSEFLLTAGHDWKVDVSGVAVSSGVPSVTFELRRMCERYLGIDPVVLGTGVKTGMP
                     VLYDNPKEVGADRIANAVGAFDLYGGPTICVDFGTATTFDVISAAGEYLGGAIIPGIE
                     ISLDALFGRAAALRRVELIEPRSVIGKTTVESIQSGVIYGYSAMIDGMCDRIEDVIGE
                     PTVIATGGLADLIAPYADAIEHVEPWLTLHGLRLIWEKNR"
     gene            893..2329
                     /gene="lysS"
                     /locus_tag="PKY13_15975"
     CDS             893..2329
                     /gene="lysS"
                     /locus_tag="PKY13_15975"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT81492.1"
                     /translation="MSTDLPYAAEVTDTAAELTSRFGELEPGIETGQTAAIAGRLMLR
                     RVQGKLAFGTIADHTGRIQLFAPSQSTPEFEAFCALSLGDWISVSGEIMTTKRGELSV
                     RVDSWTLLAATRRSFPDKWHGMTDPDTRYRQRYVDLWVTEESREAFRLRSRIVSLTRR
                     WMEDRGYMEVETPVLHPIPGGALAKPFVTHHNALDEDLYLRIAPELYLKRLVVGGFPR
                     VFEIARVFRNEGMSPRHNPEFTMLECYEAYGDYTVHMDLTEQLVAHLATELLGTTELT
                     YGDRPISLAAPWRRAPMAELLSEQIGVEVDVRTPVDELRKLCDQYDTPWKDYYGSGKL
                     LLELYEKTVEATLWEPTFVTDYPKEVSPLSRVHRDDPLLTERFEGIVAGRELCNGFSE
                     LCDPDDQRARFEDQARQNAGGDDEAMVVDDDYLRALEYGLPPTVGLGIGVDRLVMLLT
                     DSSNIRDVVLFPTLRPEQVTNTVENATE"
     gene            2336..>3017
                     /locus_tag="PKY13_15980"
     CDS             2336..>3017
                     /locus_tag="PKY13_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT81493.1"
                     /translation="MADSPAAVVLAGITKSFDGVSVLRGLDLSIAPGSVTALLGPSGC
                     GKTTALRIVAGLEDADAGTVEIGGVVASGPGINVPTEQRRVGMIFQNGALFPHLSVAH
                     NVGYGLARADRKDGPRISEMLELVGLAGFGDRRPDSLSGGQAQRVALARALAPAPAVV
                     LMDEPFSNLDAVLRARLRREVGEVVRAAGVTVLLVTHDRDEAFAVADRVAVMRDGVIV
                     QEGSPRDVY"
BASE COUNT          469 a         1042 c         1009 g          497 t
ORIGIN      
        1 gatggcctcg gccacctccg ctcaggctcg ctccctcgtc cctcgggagc ttcttacgct
       61 tcggtacctc ggcgatcccc gcaactagtc tgatcccgcc ccctatcgaa ggaatccccg
      121 tgctgctggc gatcgacgtc ggaaacaccc agaccgtcat cgggctctac aagagcggcg
      181 acgagagcca cgagctcctt gaccactggc gcatcgccac caacgccgtg cgcacctctg
      241 acgagttggc cttgatgttc agtgagttcc tgctgaccgc cggccacgat tggaaggtcg
      301 acgtgagcgg cgtcgccgtg agctccggcg tcccgagtgt cacctttgag cttcgtcgta
      361 tgtgcgagcg ctacctcggg atcgacccgg ttgtgctcgg cacgggcgtc aagacgggca
      421 tgccggtgct ctacgacaat cccaaggagg tcggcgccga ccgcatcgcc aatgctgtgg
      481 gcgcgttcga cctgtacggc ggccccacca tctgcgtcga cttcggcacc gcgacgacgt
      541 tcgacgtcat ctccgcggcg ggcgagtacc tcggtggggc gatcatcccc ggcatcgaga
      601 tcagcctcga cgcgctcttc gggcgggccg ccgcgttgcg ccgcgtcgag ctcatcgagc
      661 cgcgcagcgt gatcggcaag accaccgtcg agtccatcca gtcgggcgtg atctacggct
      721 actcggcgat gatcgacggc atgtgcgacc gcatcgagga tgtgatcggc gagccgaccg
      781 tcatcgcgac cggaggcctg gccgatctca tcgctcccta cgccgatgcc atcgagcacg
      841 tcgagccgtg gctcacactg cacggactgc gactgatctg ggagaagaac cgatgagcac
      901 cgacctgccg tacgcggccg aggtgaccga caccgctgct gagctcacct cccgattcgg
      961 cgagttggag ccgggcatcg agaccggcca gaccgcggcg attgccgggc gactcatgct
     1021 ccgtcgtgtg cagggcaagc tcgcgttcgg aacgatcgcc gatcacaccg gtcgcatcca
     1081 actgttcgcc ccgtcgcagt cgacgcccga gttcgaggcg ttctgcgcgt tgagcctcgg
     1141 cgactggatc agcgtgtcgg gcgagatcat gacgaccaag cgcggcgagc tgtccgtgcg
     1201 ggtcgactcg tggaccctgc tcgcagcgac ccgccggtcg ttccccgaca agtggcacgg
     1261 catgaccgac cccgacaccc ggtaccggca gcgctacgtc gacctctggg tgaccgagga
     1321 gtcccgcgag gcattccgcc tgcgctcgcg catcgtgtcg ctcacccggc gttggatgga
     1381 ggatcgtggc tacatggagg tggagacgcc ggtgctccac cccatccccg gcggcgcgct
     1441 ggccaagccg ttcgtcaccc accacaacgc gctcgacgaa gacctctacc tccgcatcgc
     1501 gcccgagctg tacctcaagc gcctggtcgt cggcgggttc ccgcgggtgt tcgagatcgc
     1561 tcgggtgttc cgcaacgagg gcatgtcgcc gcgtcacaac cccgagttca cgatgctcga
     1621 gtgctacgag gcgtacggcg actacacggt gcacatggat ctcaccgagc aactcgttgc
     1681 ccacctggca accgaactgc tcggcacgac cgagctgacc tacggcgacc ggcccatcag
     1741 cttggcggca ccgtggcggc gcgctccgat ggccgagctc ctcagcgagc agatcggcgt
     1801 cgaggtcgac gtgcgcacac ccgtcgacga gctgcgcaag ctctgcgacc agtacgacac
     1861 gccgtggaag gactactacg gctcgggcaa gctcctcttg gagctgtacg agaagacggt
     1921 cgaggccacg ctctgggagc cgacgttcgt caccgactac cccaaggaag tgtcgccgtt
     1981 gtcccgggtg caccgcgacg atccgctcct caccgagcgg ttcgagggga tcgtcgccgg
     2041 ccgcgagctc tgcaacgggt tcagcgagtt gtgcgacccc gacgaccagc gggcgcgatt
     2101 cgaggaccag gcgcgccaga acgccggcgg tgacgacgag gcgatggtgg tcgacgacga
     2161 ctacctgcgg gcgctcgagt acggcctgcc gcccacggtc ggtcttggca tcggcgtcga
     2221 ccgtctcgtg atgctgctga ccgacagctc gaacatccgt gacgttgtgc tgttcccgac
     2281 gctgcgtccc gaacaggtca cgaacaccgt cgagaacgcg accgagtagg tcgacgtggc
     2341 cgactcgccg gcggccgtcg tcctcgcggg catcaccaag tcgttcgacg gggtctcggt
     2401 actgcggggt ctcgacctct cgatcgcccc gggttcggtc accgcgctgc tcgggccgtc
     2461 gggctgcggc aagacgacgg ccctgcgcat cgtcgccgga ctcgaggacg ccgatgccgg
     2521 caccgtcgag atcggcggcg tggtggcgtc ggggccgggt atcaacgtgc cgaccgagca
     2581 gcgacgggtg ggcatgatct tccagaacgg ggcgctgttc ccgcacctga gcgtcgccca
     2641 caacgtgggc tatggcctgg cgcgggccga tcggaaggac ggtccccgca tctccgagat
     2701 gctcgaactc gttgggctcg ccggattcgg cgaccgccgt cccgactcgc tgtcgggtgg
     2761 gcaggcgcaa cgagtcgcgt tggcacgcgc cctggcgccg gctccggcgg tggtgctcat
     2821 ggacgagccg ttctcgaacc tcgacgccgt gctccgcgcc cggctgcggc gagaggtcgg
     2881 cgaggtggtg cgagccgccg gggtgaccgt gctgttggtc acccacgatc gtgacgaggc
     2941 gtttgccgtc gccgatcgtg tcgccgtcat gcgtgacggc gtgatcgtcc aggagggttc
     3001 gccccgcgac gtgtacg
//
LOCUS       DAOSOA010000374         3007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_32762_length_3007_cov_4.702913, whole genome shotgun sequence.
ACCESSION   DAOSOA010000374 DAOSOA010000000
VERSION     DAOSOA010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32762_length_3007_cov_4.702913"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..756
                     /locus_tag="PKY13_15985"
     CDS             388..756
                     /locus_tag="PKY13_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81494.1"
                     /translation="MPLFAVFPYQPIDWSGIGVIVGMALVPVAVTLVGVAVEGRTRSS
                     IVLTALACYCVLAVHTSQAALLVVIVGVLEVGTALERWIWPEERAGLVPWRPSQRGTP
                     LYTPGLLLLGTASSERTDFG"
     gene            1091..1600
                     /locus_tag="PKY13_15990"
     CDS             1091..1600
                     /locus_tag="PKY13_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81495.1"
                     /translation="MPRVAARTGVVAVVAGAVALGAAYLPDDVDVVRRAYSTDFLVTP
                     DLAGAFEYIDDHVADGSVVLNEERDGSAWMNAERGLSPLVGVYAYGSDEENDDRLYLV
                     AHIADLGADALVAQLLDHWSISHVLVQERGFTDEPSRPRVGDLRRNAGFCEVFSSGGP
                     QVFAVSTIR"
     gene            complement(1623..2786)
                     /locus_tag="PKY13_15995"
     CDS             complement(1623..2786)
                     /locus_tag="PKY13_15995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT81496.1"
                     /translation="MTTQPAADRGRIVIVSGIRVRNDAISNIICQQRRVLVDAGYRVD
                     VIVQHRDSDPGAWETTSPHAWDLLRNPIYLAADLVIFHFGIFYDLFNALTLAHPRARR
                     VVHFHNVTPPELLSGATRGFAQRGLVQLVTAEFADAVWSDSPHNTDVLCARTAIDPEL
                     VVPMAPSVPEVGGRQASIGTTTASTAPVRLISVGRFVPAKGLTELVAAIALLDPELRR
                     DLRLTLAGSISNSDAEYFRTLNRQIADAGLTDLIDVASDPPDDELAALLWHSDILVSA
                     SHHEGFCVPVIEALATHCRVIVTDAGALPDTAGPCGVVVPAHDAAALSVAIADEIRAV
                     KAVRAGDATAEAAERDRLDQAARHLEQFSPEQFRDRTLRAVEHELRLGAARSA"
     gene            complement(2783..>3007)
                     /locus_tag="PKY13_16000"
     CDS             complement(2783..>3007)
                     /locus_tag="PKY13_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81497.1"
                     /translation="TVVVHLPPVDDEADPGPVVTVASGDWRSVLTPSPPGFRHVTVPA
                     GAGRMITVSAVGTGASLAAVHVVPGRGRRA"
BASE COUNT          400 a         1000 c         1098 g          509 t
ORIGIN      
        1 gcgcgcgatg gcgttcttgg ccctggcgat cgtcatcggg ctggccgtct ggtgcgtcgg
       61 gctccgaggc cagccccatc agccaccgag ccgcgatggg ctctatcgcg gcttcgtcgt
      121 ggagcgtatt gtggataccg gctcgatgga tccggcgcgt gtggtggtta ccgatcccgt
      181 gaccgagacg cccgccgccg gcttccagcc gccggcgttt cccacgacgg tggccttgat
      241 gcggtcgctg gcgggtgtgc cgatcggtgt cctgctcacg gtcaccacgg tgctcgctgc
      301 cgccgtgctg cttcccgctg gcccttcacg ctcgtacggc gtctgctcct gcgccgtccc
      361 ctcgtcggcg ggttcgcggc gttggccgtg ccactgttcg cggtgttccc ctaccagccc
      421 atcgactgga gtggcatcgg tgtgatcgtc ggcatggcgc tcgtgccggt ggctgtcacg
      481 ctcgtcggtg tcgccgtcga ggggcgcacg cggtcgtcga tcgtcctgac ggccctcgcc
      541 tgctattgcg tgctcgccgt gcacacgtcg caggcggcgt tgttggtcgt gatcgtgggt
      601 gtcctcgagg tgggtaccgc gctggagcgg tggatctggc cggaggagcg ggccgggcta
      661 gtaccttggc ggccatcgca gcggggcact cctctctaca cgccgggcct gctcttgctg
      721 ggcacggcgt cgtcggagcg caccgacttc ggttgagccc cggcgctcac cctcggagcg
      781 gcgctcggcg ccctcctcac gctcacgacc gccacggcgg cgggacagtc gttgctggtc
      841 gcactcgcgt tcgtcggcat gctcgtcgac gcacgccggc gcgagctggg aggctgggcc
      901 gtggtcggtg tcgcgctggg tgctctgtac ctcgcggcgg ccgtgccgac ggcccgtggt
      961 cggtgctgcg gccactcacc cagtcctggt actacgccat caaccgcacc ggctaccacg
     1021 cgccgttggt gctcgcggtg ttcatcggat gtgcgctcga tgcagctatc gccacggtcg
     1081 ggtcgcgctc gtgccgcgtg tggcggcccg gacgggtgtg gtcgccgtcg tggcgggcgc
     1141 ggtggcgctg ggggcggcgt acctccccga cgacgtcgat gtcgtccgtc gtgcgtactc
     1201 caccgacttc ctcgtgaccc ccgacctcgc cggggcattc gagtacatcg acgatcacgt
     1261 ggccgacggc tcggtggtcc tgaacgagga gcgcgacggt tcggcgtgga tgaacgccga
     1321 acgcggcctc tcaccgctcg tcggtgtcta cgcctacggc tccgacgagg agaacgacga
     1381 ccgcctctat ctcgtcgcgc acatcgccga cctcggtgcc gacgcgctcg tggcgcagtt
     1441 gctggaccat tggtcgatca gccacgtgtt ggtccaggag cgcggcttca ccgacgaacc
     1501 gtcgcggccc cgggtcgggg acctccggcg caacgcggga ttctgtgagg tcttcagcag
     1561 cggaggaccc caggtcttcg cggtgtcgac gatccgctag ctggcatcac agccagcgct
     1621 cgtcacgcgg aacgagctgc gccgagcctg agctcgtgct cgaccgcccg gagcgtccga
     1681 tcacggaact gctcgggcga gaactgctcc aggtgccgtg ccgcctgatc cagtcggtca
     1741 cgctccgccg cctccgccgt cgcgtcgccg gcccggaccg ccttgacggc cctgatctcg
     1801 tcggcgatcg ccaccgacag cgccgctgca tcgtgggccg ggaccacgac accgcacggt
     1861 ccggccgtgt cgggcaacgc tccggcatcg gtcacgatca cccggcagtg ggtggcgagg
     1921 gcctcgatga cgggaacgca gaagccttcg tggtgcgacg ccgacaccag gatgtcggag
     1981 tgccagagga gtgcggcgag ttcgtcatcg ggcggatcgg acgccacatc gatcaggtcg
     2041 gtcagtccgg cgtcggcgat ctggcgattg agcgttcgga agtactcggc atcggagttc
     2101 gagatgctgc cggcgagtgt gagccggagg tcccggcgca gctccggatc gagcaaagcg
     2161 atcgctgcga ccaactcggt caggcccttg gccgggacga accggcccac gctgatcagg
     2221 cgcaccggag cggtggatgc ggtggtggtg cctatcgagg cttgtcggcc gccgacctcg
     2281 gggaccgaag gtgccatcgg tacgaccagc tcgggatcga tcgccgtgcg ggcgcacagc
     2341 acgtcggtgt tgtgcgggct gtcggaccac acggcgtcgg cgaactcggc ggtgacgagt
     2401 tgcacgagtc cgcgctgggc gaacccgcgc gtcgccccac tcaacagctc gggtggggtg
     2461 acgttgtgga agtgcaccac ccgacgggcg cggggatgag cgagcgtcaa cgcattgaag
     2521 aggtcgtaga agatcccgaa gtggaagatc acgagatcgg cggcgaggta gatcgggttt
     2581 cgcagcaggt cccacgcgtg gggcgaggtg gtctcccagg cgccggggtc ggagtcacgg
     2641 tgctgaacga tgacatcgac ccgatagccg gcgtcgacga gcacccggcg ttgttggcag
     2701 atgatgttgg agatcgcgtc gttgcggaca cgtatcccgc tgacgatgac gatgcgaccg
     2761 cgatcggcgg ccggctgcgt ggtcatgccc gacgtccacg gcccgggacg acgtggaccg
     2821 cagccaacga tgcgccggtg ccgacggcgg agacggtgat catgcgcccg gcgcccgccg
     2881 ggacggtgac atgtcggaac ccgggtggtg acggggtgag caccgaccgc cagtcgcccg
     2941 acgcgaccgt gacgaccggg ccagggtcgg cctcgtcgtc gacgggaggg aggtgcacga
     3001 ccacggt
//
LOCUS       DAOSOA010000375         2974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_33218_length_2974_cov_5.784173, whole genome shotgun sequence.
ACCESSION   DAOSOA010000375 DAOSOA010000000
VERSION     DAOSOA010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33218_length_2974_cov_5.784173"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1400
                     /locus_tag="PKY13_16005"
     CDS             21..1400
                     /locus_tag="PKY13_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT81498.1"
                     /translation="MIPVVTPDEMAAIDAAASVPVETLIERAGAAVARAALQMLGGAY
                     GRRVVVIAGKGNNGRDGAVAARLQRSRGCRVTVVDAAADATVPECDLVIDAAYGTGFR
                     GSYRSPVSAGVPVLAVDIPSGVDGLTGVASGRPAVAERTVTFAALKPGLLYADGAATV
                     GTTMVADIGLDVSGARAHVVEDGDVGRLLRPRPVDAHKWKAAVWVVGGTPDMAGAPVL
                     ATAGAFAAGAGYVRLSSPGASALRAAPLEAVGAPLPMIGWERTVLADSERVSSLVVGP
                     GLGRNDAVRDSVCSLATSSSLPMVIDGDALWAIAGLRGRMSDAGRVLTPHDGEYTQLT
                     GHPPGADRIDAARRLAAERGSVVLLKGPTTTVASPTGEVRIVVAGDTRLATAGTGDVL
                     SGVIGALLASGLDAFDAAAVGAQAHALAARRCPTVGTTASMLPPMVSAVLSDALAAKA
                     ARPRRVERQ"
     gene            1397..2527
                     /gene="alr"
                     /locus_tag="PKY13_16010"
     CDS             1397..2527
                     /gene="alr"
                     /locus_tag="PKY13_16010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT81499.1"
                     /translation="MTTRTYRPTRAEIAPGAIRRNVERLGVLVAPAAVCAVVKGDGYG
                     HGIVTAATAALEGGATWLGVALAEEAFALRDAGFTVPVLLLSEPVAAAWGDLVAGDID
                     VAVYSQAAIGAAQSAAASATRVAALHLKVDTGMHRVGCAPGDAIGLARAVHESPRSRL
                     AGLFTHFATADDPASMYPAIQLERYRQVDDALADAGIGGYLRHAANSAGAIAVAGSRF
                     DLVRCGIACYGIAPSPALQGRVELEPALRLVSAVSHVKRLGGGEPVSYGCRFVTERET
                     TVITVPIGYADGLPRSLGLNGGYVLVGGRRRPVVGTVTMDQLMVDAGDLPVAVGDEVV
                     LLGTQGDDEIDANEVAAREGSIGYEIVTRLGPRVPRVTVGPN"
     gene            complement(2536..>2974)
                     /locus_tag="PKY13_16015"
     CDS             complement(2536..>2974)
                     /locus_tag="PKY13_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81500.1"
                     /translation="AIEGWYGARVLCGNFRPPKVAMTNTLFAVGSKPTKESNLAGDAV
                     MAGATLPFSGNGCTGTEVEYDISGGARWPGPFEADGTIAVNPDGTWSTDLLFPTDMNP
                     GPASVRARCVLANQYDEVVYVYYGGMTEVTVERPPPATLAPPA"
BASE COUNT          390 a         1031 c         1098 g          455 t
ORIGIN      
        1 actgttgaca caatgcaggc gtgatccccg tcgtgacgcc cgatgagatg gccgcgatcg
       61 acgctgcggc gtcggtgccg gtcgagacgc tgatcgaacg cgccggggct gctgtcgctc
      121 gggccgcgct ccagatgttg ggcggggcgt acgggcgacg cgtcgtcgtg atcgccggaa
      181 agggcaacaa cggccgcgac ggagcggtcg ctgcacgact gcagcggtcg cgggggtgtc
      241 gggtcaccgt ggtcgatgcc gctgccgatg cgacggttcc ggagtgtgac ctcgtgatcg
      301 acgctgccta cggcaccggg tttcggggct cgtaccgctc accggtatcg gccggcgttc
      361 cggtcctcgc cgtagacatc ccgtccgggg tcgacggcct cacgggtgtc gcatccggac
      421 gccccgcggt cgccgagcgc accgtgacct tcgccgcgct gaagccgggg ttgctctacg
      481 ccgatggggc ggcgacggtg ggaacgacga tggtcgccga tatcgggttg gacgtctcgg
      541 gggcacgagc ccacgtggtc gaggacggtg acgtcggccg cctgctccgg ccccgccccg
      601 tcgacgccca caagtggaag gccgcggtgt gggtcgtcgg gggaaccccc gacatggccg
      661 gggcgcccgt gctggcgacc gccggagcgt tcgccgccgg tgccgggtac gtgcgactga
      721 gctcgccggg cgcgagcgcc ctgcgggcgg ccccgctcga ggcggtcggc gccccgctcc
      781 cgatgatcgg gtgggagcga acagtcctgg ccgacagcga gcgcgtgtca tcgctggtcg
      841 tcgggcctgg cctggggcgc aacgatgcgg tccgagattc ggtctgttcc ctcgcgacct
      901 cctcgagcct gccgatggtg atcgatggcg acgccctgtg ggccatcgcc ggcctccgcg
      961 gtcgtatgtc cgacgccggt cgcgtgctga caccccacga cggcgagtac acccagctga
     1021 ccgggcatcc tcccggagcc gatcgaatcg atgcggcccg acggctcgcc gccgagcgtg
     1081 gctcggtcgt tctgctgaaa ggtccgacga ccaccgtggc ctcgccgacg ggagaggtgc
     1141 gaatcgtcgt cgccggcgac acccggttgg caaccgcagg gaccggcgac gtgctcagcg
     1201 gagtgatcgg cgcgctgttg gcctcgggcc tcgacgcgtt cgatgccgca gccgtcggag
     1261 cacaggcgca cgcgctggcc gcccgtcgct gcccgacggt gggaacgacg gcgtcgatgt
     1321 tgccgccgat ggtgtccgcc gtgctgagcg acgcgctggc ggccaaggcc gcccgcccgc
     1381 gccgcgtgga gcggcagtga cgacccgcac ctaccgcccc acccgcgccg agatcgctcc
     1441 cggcgcgatt cgccggaacg tggagcggct cggagtgctc gtcgccccgg cagccgtgtg
     1501 tgccgtggtg aagggtgacg gatacgggca cgggatcgtc accgctgcga cagcggcgct
     1561 cgagggcgga gcgacatggc tcggggtcgc gctcgccgaa gaggcgttcg ccctgcgcga
     1621 tgccggcttc accgttcccg tgctcctcct gagcgagccc gtagcggcag cgtggggcga
     1681 tctggttgcc ggcgacatcg acgtggccgt gtactcccaa gccgccatcg gcgccgctca
     1741 gtcggcggcg gcgtcggcga cccgggtcgc tgcgctccac ctcaaggtcg acacgggtat
     1801 gcaccgtgtc gggtgcgctc ccggcgacgc gatcggcctt gctcgggccg tccatgagtc
     1861 gccccggtcg cgtctcgccg gactcttcac gcacttcgcc acggccgacg atccggcatc
     1921 gatgtacccc gcgatccaac tcgagcggta ccggcaggtg gacgacgcgc tcgccgacgc
     1981 cggcatcggc ggctacctgc gccacgccgc gaactcggcg ggggcgatcg ccgtcgctgg
     2041 gtcgcggttc gatctcgtcc ggtgtgggat cgcgtgctac ggcatcgcgc cgtccccggc
     2101 gttgcagggg cgggtcgaac tcgagccggc cctccggctc gtgtcggccg tgagtcacgt
     2161 gaagcgcctc ggcgggggag agcccgtgag ctacggctgc cggttcgtca ccgaacggga
     2221 gacgacggtc atcaccgtcc ccatcggata tgccgacggc ctgcctcgat cgctcgggct
     2281 caacggtggc tacgtgctgg tgggcggacg tcgtcgaccg gtggtcggca ccgtgaccat
     2341 ggatcagctg atggtcgacg ccggtgacct cccggtcgcg gtcggcgatg aggtggtcct
     2401 gctcggaacg caaggcgacg acgagatcga cgcgaacgag gtggcagcgc gggagggcag
     2461 catcggctac gagatcgtga cgcgactggg accgcgggtt ccccgggtca cggtcggacc
     2521 gaactgacgc tcgcctcagg ccgggggagc gagcgtcgct ggtggcggtc gctcgaccgt
     2581 cacctccgtc atcccgccgt agtagacgta cacgacctcg tcgtactggt tggcgagcac
     2641 acagcgggcc cgcaccgatg ccggccccgg gttcatgtcg gtcgggaaga gcaggtcggt
     2701 cgaccaggta ccgtccgggt tcacggcgat cgtgccatcg gcctcgaacg ggccgggcca
     2761 ccgggcgccc ccgctgatgt cgtactccac ctcggtcccg gtgcagccat ttcctgagaa
     2821 cggcagcgtc gcaccggcca tgacggcgtc gccggcgagg ttcgactcct tcgtcggctt
     2881 cgaaccgacg gcgaagagcg tgttcgtcat cgccactttg ggcggccgga agttgccgca
     2941 caggacacgc gctccgtacc acccttcgat cgcg
//
LOCUS       DAOSOA010000376         2937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_33743_length_2937_cov_6.065232, whole genome shotgun sequence.
ACCESSION   DAOSOA010000376 DAOSOA010000000
VERSION     DAOSOA010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33743_length_2937_cov_6.065232"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKY13_16020"
     CDS             complement(<1..528)
                     /locus_tag="PKY13_16020"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT81501.1"
                     /translation="MARRTGPRWGRIAGLGLCAFVIGSLAGWIFGGADDATVSTADPT
                     TTTLRADGGTDAPPSTGATSTTVDPAAPVRDQIGAGQPVTFVFGGDIHFDGPLATTLA
                     NAPDSILAGLQPTLSDADVAVVNLETAIGTGGTKAAKEFNFRASPAAFGALTSAGVDV
                     IGMANNHALDYGQDGL"
     gene            677..>2937
                     /locus_tag="PKY13_16025"
     CDS             677..>2937
                     /locus_tag="PKY13_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HOT81502.1"
                     /translation="MSRESLGGVPVQLTTKRTSPAARSGFPAAQGLYSPENEHDACGV
                     SFVAQIDGTRSHRLVQQAIGSLCNLEHRGATGAEVNTGDGAGILIQIPDRFLREVVDF
                     ELPPEGAYAVGLAFLPSSTADTDDAADAVGKIVDSEGLTVLGWRDVPTDPLGADIGYS
                     ALGVMPTFRQVFISSPEGLTGIDLDRRVFVARKRVEHELVDEAGDPLVYFPSLSARTL
                     VYKGMLTTPQLALFFPDLQDERVESAIGLVHSRFSTNTFPSWPLAHPYRFVAHNGEIN
                     TVQGNQNWMRAREAMVQSPHFGDGDISRVFPICTPGASDTARFDEALELLHLGGYDLP
                     HAVLMMIPEAWENHADMTPERKAFYRYHSCLMEPWDGPASVVFTDGTLVGAVLDRNGL
                     RPSRYWVTDDGLVVMASEVGTLDIDQATVVQKGRLQPGKMFLIDTAQGRIISDQEIKD
                     QLASALPYQQWLDDNLVHLDNLPDVIHITESELETRREQQTFGYTHEELKLIVDPMAK
                     AGYEALGSMGTDTPLAVLSNRPRLMFDYFAQLFAQVTNPPLDAIREELVTSVGRMVGP
                     EGNVLDPQPHSARLIELRRPILDNDELAKLEHVEEAHLGFRTKRISCLYPVAEGGEGL
                     RAALARIREEASEAIAEGYSLLILSDWRSDETMAPVPSLLFTSAVHHHLIRNKTRTQV
                     GLIVETGEAREIHHFCLLIGFGAAAVNPYLAFDTIEDRIRNGIIEGIDFRTAVENYIK
                     AASKGILKVMSKMG"
BASE COUNT          469 a         1051 c          962 g          455 t
ORIGIN      
        1 gagtccgtcc tgcccgtagt cgagggcgtg gttgttggcc atgccgatga cgtcgacacc
       61 ggcgctcgtc agggcaccga acgcggcggg ggacgcgcgg aagttgaact ccttcgctgc
      121 cttcgtgcct ccggttccga tggcggtctc gaggttgacg accgcgacgt cggcgtcgct
      181 cagcgtgggt tggaggccgg ccaggatcga gtccggggcg ttcgccagcg tggtggcgag
      241 gggcccgtcg aagtggatgt cgcccccgaa gacgaacgtc acgggctgac cggccccgat
      301 ctgatcgcgc accggagccg ccggatccac ggtggtcgac gtcgcaccgg tggagggcgg
      361 cgcgtcggtg ccgccgtcgg cacgaagggt cgtcgtggtc ggatcggcgg tggacacggt
      421 ggcgtcgtcg gcaccgccga agatccagcc cgccaggctg ccgatcacga acgcgcacag
      481 gccgaggccg gcgattcgac cccagcgggg tccggtccta cgcgccatgg gggcaaacct
      541 accggtaact cgtttgtgat ggcgtcgcgc gtaccgggta tcctgttgca cctctcccag
      601 ggccaacggc gtccctggcg tgaccgcaat cttcgtcggc aacgggccgg cggatccgat
      661 ccaccagggg agaacgatgt ccagagaatc tcttggcggc gtacccgtcc agctcaccac
      721 gaagcgcaca tcgccggctg cccgcagcgg tttccccgcg gcgcagggcc tctactcgcc
      781 cgagaacgag cacgacgcgt gtggtgtgtc cttcgtcgcc cagatcgacg gcacccgcag
      841 ccaccgcctc gtgcagcagg cgatcgggtc gctgtgcaac ctcgagcacc gtggcgccac
      901 gggcgccgag gtcaacaccg gcgacggggc cggcatcctc atccagatcc ccgaccggtt
      961 cctccgcgag gtcgtcgact tcgagttgcc gcccgagggc gcctatgccg tcggcctggc
     1021 cttcctgccg agcagcaccg ccgacaccga cgacgccgcg gatgccgtgg gcaagatcgt
     1081 cgattccgag gggctgaccg tgctcggttg gcgcgacgtc ccgaccgacc cgcttggtgc
     1141 cgacatcggc tactcggcgc tcggcgtcat gccgaccttc cgccaggtgt tcatcagctc
     1201 gcccgagggc ctcaccggca tcgacttgga ccgtcgcgtc ttcgtggccc gcaagcgcgt
     1261 cgagcacgag ctggtcgacg aggccggcga tccgctcgtg tacttcccgt cgttgtcggc
     1321 gcggacgctc gtctacaagg gcatgctgac gacgcctcag ctggcgctgt tcttccccga
     1381 tctgcaggac gagcgggtcg aatcggccat cggcctcgtg cattcgcggt tctcgacgaa
     1441 cacgttcccg tcgtggccgc tcgcacaccc gtaccgcttc gtcgcccaca acggcgagat
     1501 caacacggtg cagggcaacc agaactggat gcgtgcccgc gaggccatgg tccagagccc
     1561 ccacttcggc gacggcgaca tcagccgggt gttcccgatc tgcacccccg gcgcgtccga
     1621 tacggcacgg ttcgacgagg ccctcgagct gttgcacctc ggcgggtacg acctgcccca
     1681 cgccgtgctc atgatgatcc ccgaggcgtg ggagaaccac gccgacatga cgccggagcg
     1741 caaggcgttc taccgctacc actcctgcct catggaaccg tgggacggcc cggcctcggt
     1801 cgtgttcacc gacggcaccc tcgtgggcgc ggtgctcgac cgcaacggtc tgcgcccgag
     1861 ccgctactgg gtgaccgacg acggcctcgt cgtcatggcg tccgaggtcg gcacgctcga
     1921 catcgatcag gcgaccgtcg tgcagaaggg tcgcctccag cccggcaaga tgttcctgat
     1981 cgacaccgcc cagggccgca tcatcagcga ccaggagatc aaggaccaac tggcgtcggc
     2041 gctgccgtac cagcagtggc tcgacgacaa cctggtgcac ctcgacaacc tgcccgacgt
     2101 catccacatc accgagtccg agctcgagac ccgccgcgag cagcagacgt tcgggtacac
     2161 ccacgaggag ctcaagctca tcgtcgaccc gatggcgaag gccgggtacg aggcgctggg
     2221 gtcgatgggc accgacacac ccctggctgt gctctccaac cggccgcggc tcatgttcga
     2281 ctacttcgcg cagctgttcg cgcaggtcac gaacccgccg ctcgacgcca tccgcgagga
     2341 gctcgtcacg agcgtcggcc gtatggtcgg ccccgagggc aacgtgctcg acccccagcc
     2401 gcacagcgcc cgcctcatcg agctgcggcg tccgatcctc gacaacgacg agctggccaa
     2461 gctcgagcac gtcgaggaag cccatctcgg cttccgcacc aagcgcatca gctgcctgta
     2521 tcccgtcgcc gagggtggcg agggcctgcg cgctgcgctc gcccgcatcc gcgaggaggc
     2581 gtcggaggcg atcgccgagg gctacagcct gttgatcctc agcgactggc ggtccgacga
     2641 gaccatggcc ccggtgccgt cgctgctgtt cacctcggcg gtgcaccacc acctgatccg
     2701 caacaagacc cgcacccagg tcggcctcat cgtcgagacg ggcgaggctc gcgagatcca
     2761 tcacttctgc ctgctcatcg ggttcggcgc cgccgcggtc aacccgtacc tggcgttcga
     2821 cacgatcgag gaccggatcc gcaacgggat catcgagggc atcgacttcc gcacggcggt
     2881 cgagaactac atcaaggccg cctccaaggg catcctcaag gtcatgtcca agatggg
//
LOCUS       DAOSOA010000377         2919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_34041_length_2919_cov_3.319134, whole genome shotgun sequence.
ACCESSION   DAOSOA010000377 DAOSOA010000000
VERSION     DAOSOA010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34041_length_2919_cov_3.319134"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2645)
                     /locus_tag="PKY13_16030"
     CDS             complement(<1..2645)
                     /locus_tag="PKY13_16030"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOT81503.1"
                     /translation="MTPNRIIQTGRRPQRVQIAGAFAAVLVMTAGLVATAPDVSAGAV
                     FTVTTTADSGAGSLRDAIIAANADATATPAAPHQIGFAIPGSGVQTINVLSALPAITR
                     PTVIDGLSQPGATCGDSGAPRQLLVAIRGRGNALAMPGLVLTGGASTVQGLAVSFFRA
                     GDVITTGAAGGDTIRCNHIGTNVAGTLTDSVTSNGGSLTGNPVTTAALDVSSPATVVG
                     GRSHTAGVCDGDCNVIAGESDNNNQLSILLRFGSLTSTGFNGLTSTSSAAGGTMQGNF
                     IGIAQNGQNFPTTKKAGYHILMVSSDSASAAAPAVPQPIPSSGDYLIGGLDAGGDRDP
                     LAGNVISGAKHRDGIDCNTATGCASGVRIHGNLIGVGPTGAEATDLAGITYGNARDGI
                     QLEGQHSADIVANVVSGNGSNGINAGSLTNDTNIADNTVGLTTNGLADGNGWYSTARI
                     AGSPDFGLGAVSTNTNAGSGNGISIGAYGCGIPNRDCTNLTVSGNVVGNNARAGVIAL
                     NGTGNMSLTDNFIGTDRSGTPAPNGAAGVVVMTAGNRITGNVIAANGGPGVTVLRNTN
                     ALMGSVVGAYTPVPATATDNVISRNSIFDNVGLGIDLASLSATWNGSGNQLLFPYTWT
                     RGVTPDDGLLSAAPPTNAATFGNRDVDYPVITSAVVSASANTVTVGGHVGMAGGSAVF
                     GGSTVEVFAGDDTPADQSGEIEAGDGLAVAHPEGRTFIGDCPTTAGGTFSGCVLSLPP
                     SLPASSWASGGRLTSTATLASATSEFGPQLDPVVLGAISGTVFLDDTGAPIEGVTLTL
                     LDHNSAVVAVATTAADGTYSFADLPAGDYTVVETQPAGHLDGVVTPGNQLAITVIAGS
                     VASGNDFSEALPPPPTTTTTSTTTEP"
BASE COUNT          393 a          927 c         1113 g          486 t
ORIGIN      
        1 ggttcggtgg tggtggaggt cgtcgtggtc gtcggaggcg gcggaagggc ctcgctgaag
       61 tcgttgccgc tcgcgacgct gccggcgatc acggtgatgg cgagttggtt gcccggtgtc
      121 acgacaccgt cgagatggcc ggcgggttgg gtctccacca cggtgtagtc accggcgggc
      181 aggtcggcga aggagtacgt gccatccgct gcggtcgtcg cgacggcgac caccgcactg
      241 ttgtggtcga ggagcgtgag ggtcaccccc tcgatcggtg cgccggtgtc gtcgaggaac
      301 accgttccgg agatggcacc gagcaccacg ggatcgagct ggggcccgaa ctccgacgtg
      361 gccgatgcga gtgtggcggt cgaggtcagt cgaccacccg acgcccacga cgatgccgga
      421 agcgacggcg gcagcgacag cacgcacccc gagaaggtgc caccggccgt cgtgggacag
      481 tccccgatga acgtgcgtcc ctcggggtgt gccaccgcga gtccgtcgcc ggcctcgatc
      541 tcgccgctct ggtccgccgg tgtgtcgtcg ccggcgaaca cctcgaccgt cgacccaccg
      601 aacaccgctg atccgccggc catcccgacg tggccgccga cggtcacggt gttcgccgac
      661 gcggacacga ccgccgacgt gatgacgggg tagtcgacgt cccggttgcc gaacgtcgcg
      721 gcgttcgtcg gcggagcagc agacagcagg ccgtcgtcgg gtgtcacacc acgggtccac
      781 gtgtagggga acaggagctg gttgccgctg ccgttccagg tcgcactgag cgaggcgagg
      841 tcgataccga ggccgacgtt gtcgaagatc gagttcctcg agatcacgtt gtcggtcgcc
      901 gtcgccggca ccggggtgta cgcgcccacg acgctgccca tgagcgcgtt ggtgttgcgc
      961 agcaccgtga ctcccggtcc gccattggcg gcgatgacgt tgccggtgat gcggttgccg
     1021 gcggtcatga caaccacgcc ggccgctccg ttgggtgcag gagtgccgga gcggtcggtg
     1081 ccgatgaagt tgtcggtcaa cgacatgttc ccggttccgt tgagggcgat cacgcccgct
     1141 cgagcgttgt tgcccacgac gttcccgctg acggtcaggt tggtgcagtc ccggttggga
     1201 atgccgcagc cgtacgcgcc gatcgagatg ccgttgcccg atccggcgtt ggtgttggtc
     1261 gaaacggcgc cgagcccgaa gtcgggtgag cccgcgatgc gggcggtcga gtaccagccg
     1321 ttgccgtcgg cgagtccgtt cgtcgtgagc ccgacggtgt tgtcggcgat gttcgtgtcg
     1381 ttggtcaggg agccggcgtt gatgccgttg ctgccgttgc cggacacgac gttggcgacg
     1441 atgtcggcac tgtgctgtcc ctcgagctgg atgccgtcgc gggcgttgcc gtaggtgatg
     1501 ccggcgagat cggtcgcctc ggcacccgtc ggaccgacgc cgatgaggtt gccgtggatc
     1561 cggactcccg acgcgcaccc ggtcgcggtg ttgcagtcga tgccgtcgcg gtgcttcgcc
     1621 cccgagatca cgttgccggc gagtgggtcg cggtcgccgc cggcgtcgag gccgccgatc
     1681 aggtagtcgc ccgacgacgg gatgggctgg gggaccgccg gtgccgcggc cgaggcggag
     1741 tcggaggaca ccatcaggat gtggtagccc gccttcttcg tggtcgggaa gttctggccg
     1801 ttctgggcga tgccgatgaa gttgccctgc atcgtgccgc ccgccgcgct cgaggtgctg
     1861 gtgagtccgt tgaaacccgt cgaggtcaac gacccgaacc gcagcaggat gctcagctgg
     1921 ttgttgttgt cggactcgcc ggcgatcacg ttgcagtcac cgtcgcagac gccggcggtg
     1981 tgcgaccgtc ccccgacgac ggtggcgggt gagctcacgt cgagcgctgc ggtcgtcacg
     2041 gggttgcccg tgagcgagcc gccgttcgac gtcaccgagt cggtgagcgt tccggccacg
     2101 ttggtcccga tgtggttgca ccggatggtg tcgccgcccg ccgccccggt cgtgatcacg
     2161 tcgccggccc ggaagaacga cacggcgagt ccctgcacgg tcgacgcgcc gccggtcagg
     2221 acgagtcccg gcatggcgag ggcgttgccg cgcccgcgga tcgccacgag gagctgtcgg
     2281 ggcgcgcccg agtcgccgca ggtggcaccg ggctgggaca agccgtcgat cactgtcggc
     2341 cgggtgatcg cgggcagcgc actcagcacg ttgatggtct gcactccgga gccggggatc
     2401 gcgaacccga tctggtgggg cgcggcgggc gtcgcggtcg cgtcggcgtt ggcggcgatg
     2461 atggcgtcgc gtagcgaccc ggcgccgctg tcggcggtgg tggtgaccgt gaagaccgct
     2521 ccggccgaca cgtcgggtgc ggtggcgacc agccctgcgg tcatcacgag gacagcggcg
     2581 aacgctccgg cgatctggac gcgctgcggg cggcggccgg tctggatgat tcgattgggc
     2641 gtcaccttca cgtgttcggt tgggcccggt cgcgagatga ctccgaagcg caggaatggg
     2701 tccagtgcgg gaacatttgc ccacgaggcg ccatcgtgcc cacggcgggg gcgatgagcc
     2761 cggccggtgg gcctcggacg gcctcagtcg tcggctgcga gccgctcggc gagtccgtcg
     2821 aggaggaggt cgaggccgta cgcgaactcg tctccgaagt cgtaccccgg ggcgagcacg
     2881 tgcgtctcgg tgaactcggc gaggtggggg tacgcgccg
//
LOCUS       DAOSOA010000378         2890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_34495_length_2890_cov_5.946032, whole genome shotgun sequence.
ACCESSION   DAOSOA010000378 DAOSOA010000000
VERSION     DAOSOA010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34495_length_2890_cov_5.946032"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1355)
                     /locus_tag="PKY13_16035"
     CDS             complement(9..1355)
                     /locus_tag="PKY13_16035"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HOT81504.1"
                     /translation="MVNADETRSAAMSESWGFETRQIHAGQEPDSATGARAVPIYQTT
                     SYQFRDSEHASNLFALAEIGNIYTRIMNPTQGVLEARLSSLEGGTATAVGLPGALVVS
                     SGQAAETLAILNVAEAGDHIVASPSLYGGTYNLLHYTLPKIGIEVSFVDDPHDLDQWR
                     AASKANTKAFFGEVLPNPKNDVFDIEGVSAVAHEVGVPLIVDNTVASPYGINPLKWGA
                     DIVVHSTTKFIGGHGTSIGGVIIDGGTFDYGASGRHPGLTEPDPSYHGLAYYPALGNG
                     AYIIKARVQLLRDLGPAISPFNAFLTLQGVETLSLRMERHWQNADKVARFLDAHPQVS
                     SVSYAGLESSPWHERAKKYFEGRGFGSVLAFEIEGGREAGRKFADSLQLHSHVANIGD
                     VRSLVIHPASTTHSQLTPDEQLASAVTPGLVRLSVGLESIDDILADLEIGFAAAKE"
     gene            complement(1413..2228)
                     /locus_tag="PKY13_16040"
     CDS             complement(1413..2228)
                     /locus_tag="PKY13_16040"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOT81505.1"
                     /translation="MRVSVPASSANLGPGFDALGLALDLPFLLSNSPGGKALEAEPTH
                     PASVAFARAGGRGPLWWKSPIPPGRGLGFSGSARVAGALMGLLQAGRPEAEAKPIALG
                     IACELEGHPDNAAASMLGGITTAAGGRAVRISTPLDLSVVVWWPSSSTSTNQARAALP
                     STVPFEDAVFNVGRSSLLVAALAAGDVASLATATEDRLHQQARLAMSPVSAVALDALR
                     GAGVLAAWLSGSGPTVAAFVWPDDAERVVAALPADGTARILRIDTIGAHSVRT"
     gene            complement(2249..>2890)
                     /locus_tag="PKY13_16045"
     CDS             complement(2249..>2890)
                     /locus_tag="PKY13_16045"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT81506.1"
                     /translation="VLRGEFEKPYWPELRSFVAAERAAATVYPPVEQEFAALHLTPYA
                     DTRVLILGQDPYHGPGQAHGLCFSVQPGVKPPPSLENIFKEMETDVGCPRPSHGNLEA
                     WARQGVLLLNAVLTVRAHHAGSHANKGWETFTDRIIATVSAKETPVVFILWGAYARKK
                     KVLIDQSRHTIIESAHPSPLSARNGFFGSRPFSRANEALVAAGLQPIEWCLGA"
BASE COUNT          462 a          965 c         1026 g          437 t
ORIGIN      
        1 gcccggtgct actccttcgc cgcggcgaag ccgatctcga ggtcggcgag gatgtcgtcg
       61 atcgactcca ggcccaccga caggcggacc aggccgggcg tgacagcgct ggccagctgc
      121 tcgtcgggcg tgagctggct gtgcgtggtc gatgccggat ggatgacgag cgagcggacg
      181 tcgccgatgt tggcgacgtg gctgtggagc tgcagcgagt cggcgaactt gcggccggcc
      241 tcgcggccac cctcgatctc gaacgccaac accgagccga agccgcgacc ttcgaagtac
      301 ttcttcgccc gctcgtgcca cggcgacgac tccaggccgg cgtagctcac cgagctcacc
      361 tggggatggg cgtcgaggaa cctcgcgacc ttgtcggcgt tctgccagtg gcgctccatg
      421 cgcaggctca gcgtctcgac gccctgcagg gtgaggaacg cgttgaacgg cgagatcgcg
      481 gggccgaggt cgcgcaacag ctgcacgcgg gccttgatga tgtacgcgcc gttgccgagg
      541 gccgggtagt aggcgagccc gtggtagctc gggtcgggct cggtcaggcc ggggtgacga
      601 cccgacgccc cgtagtcgaa ggtgccgccg tcgatgatca cgccaccgat cgacgttccg
      661 tgaccgccga tgaacttggt ggtggagtgc acgacgatgt cggcacccca cttgagcggg
      721 ttgatgccgt agggcgatgc gacggtgttg tcgacgatga ggggcacccc gacctcgtgg
      781 gccacggccg agacgccctc gatgtcgaac acgtcgttct tcgggttcgg caggacctca
      841 ccgaagaacg ccttggtgtt cgccttcgag gcagcgcgcc actggtcgag gtcgtggggg
      901 tcgtcgacga agctcacctc gatgccgatc ttgggcagcg tgtagtgcag caggttgtag
      961 gtgccgccgt agagcgacgg cgacgcgacg atgtggtcac cggcctcggc gacgttgagg
     1021 atcgcgagcg tctcggccgc ctggcccgac gacacgacca gggcacccgg aaggccgacg
     1081 gccgtggccg tgccgccctc gaggctcgag agtcgcgcct cgagcacccc ctgcgtcggg
     1141 ttcatgatgc gggtgtagat gttgccgatc tcggcaagcg cgaacaggtt cgacgcgtgc
     1201 tcggaatcgc ggaactggta cgacgtcgtc tggtagatcg ggacggcccg cgcccccgta
     1261 gcgctgtccg gttcctgccc ggcatggatc tgacgggttt cgaaacccca cgactcgctc
     1321 attgctgcac tcctggtctc gtcggcgttg acgatgggtg accctaggag tcaaaagccg
     1381 accaaacaag tcagatttat ggagcgtgcc ggtcaggttc ggacggagtg ggcgccgatc
     1441 gtgtcgatcc gcaggatgcg ggccgtgccg tccgccggga gcgccgcaac gacccgttcg
     1501 gcatcgtcgg gccacacgaa cgccgccacc gtgggacccg agccgctcaa ccacgccgcc
     1561 aacaccccgg cgccgcgaag cgcatcgagc gcgactgcgg agaccgggga catcgccagg
     1621 cgagcctgct ggtggagtcg atcctcggtg gcggtcgcaa gcgaagcgac atcgccggcc
     1681 gccagcgccg cgaccagcag cgaactacgg cccacgttga acacggcatc ctcgaacggc
     1741 accgtcgagg gcagggccgc tcgagcctgg ttcgtcgagg tcgacgagga cggccaccac
     1801 acgaccacgc tcaggtcgag cggcgtcgag atccgcacgg cgcgcccgcc ggccgccgtg
     1861 gtgatgccac cgagcatcga cgccgcggcg ttgtcggggt gtccctccag ctcacaggcg
     1921 atgcccaggg cgatgggctt cgcctcggcc tcggggcgtc ccgcctgcag cagccccatg
     1981 agcgcgccgg caacccgagc cgagccggag aagccgagac cgcgcccggg cgggatcggg
     2041 ctcttccacc acagcggccc cctgccgccg gcacgggcga acgccacgga cgcagggtgg
     2101 gtcggttccg cctcgagcgc cttgccaccc ggcgagttcg acaagaggaa cggcaggtcg
     2161 agcgcaagac cgagtgcgtc gaagccgggg ccgaggttgg cggacgatgc cgggacgctg
     2221 actcgcatcc gcccacccta cgagcagctc acgctcctag acaccactcg atgggctgca
     2281 ggccggcagc gacgagggct tcgttggccc gggagaacgg tcgactgccg aagaagccgt
     2341 tgcgcgccga caggggcgac gggtgcgccg actcgatgat cgtgtgccgc gactggtcga
     2401 tcagcacctt cttcttgcgg gcgtacgctc cccacaagat gaacacgacg ggcgtttcct
     2461 tcgccgacac cgtcgcgatg atccggtcgg tgaaggtctc ccaccccttg ttggcgtggg
     2521 agccggcgtg gtgggcccgc accgtgagaa ctgcgttgag caacaggacg ccctgtcggg
     2581 cccacgcttc gaggttgccg tgcgatgggc ggggacaccc gacgtcggtc tccatctcct
     2641 tgaagatgtt ctcgagcgag ggcggcggct tcactcccgg ttgtaccgag aagcacagtc
     2701 cgtgggcctg ccccggaccg tggtacgggt cctgcccgag aatcaacacg cgggtgtccg
     2761 cgtagggcgt caggtggagt gcggcgaact cctgctcgac cggtgggtac accgtcgcgg
     2821 ccgcacgctc cgcggcgacg aagctgcgca gttcgggcca gtacggcttc tcgaactcac
     2881 cacgcagcac
//
LOCUS       DAOSOA010000379         2886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_34549_length_2886_cov_6.420346, whole genome shotgun sequence.
ACCESSION   DAOSOA010000379 DAOSOA010000000
VERSION     DAOSOA010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34549_length_2886_cov_6.420346"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2886
                     /locus_tag="PKY13_16050"
     CDS             <1..>2886
                     /locus_tag="PKY13_16050"
                     /inference="COORDINATES: protein motif:HMM:NF012323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWD domain-containing protein"
                     /protein_id="HOT81507.1"
                     /translation="AVGEEGYTDIAAPEWKPATAAEDLAWREETDGLDAQIAVDAFDL
                     AFGDMPGATERELDEPGSVSATRAWGLVEHFADDLTPEQTRRVAEVRAERGTGTPLPD
                     AAGELPPEWEALADGATTPGATAPPSENAPPPGAPAPGAAAPPSDSATTTTARTSSLR
                     NLRAPAAAGFARKAAPDAALQTILNGLMTRIIIGWRNRLGNVGLPSVSSSYSNTGLTT
                     ATGLDAYATATPNGDGCLITIYPLLYEHIVKLTPAYVAFVLAHELFHCVEFEWAPGFW
                     MTAPDWVVEGGADFAGYDYYRKQVKVDDQFIEWFTSRNTPLASRSYSGAGLWEIAAAE
                     GIDPYSSIKVALRATGNTETVLAAAGLNSPSLLLDWPSRTVRSQAYTHPSWTMPWTGA
                     GDGRHENFEKGATITLGGTNVRGPANYSHALQAHVFGPGVDIVTVRNKGKHLVSLAND
                     RENLVLGSNETMRLCVDESRCTCPDGNYGSLRFFNSTDVAVGFSASREAGLVTVLAEK
                     FDKKRHCEKQRPRGSGDGDPHLLTMDGIAFDVMSVGEFVLASTPEADDTPAFDLQMRT
                     AAAATAGDRYSLITALAVDYGDDRITLTAADYLSKPDEIVVRHNGDTADIDGGTLGAF
                     VLTRDDERNWTLSAPDGPELRLKYLNGFFVDVAPPETTAAQQVGMLGSANDDPLDDVR
                     RADGTPVNAFDPVEVHGAFADSWRVTPGTSRFDYLPGESTDTFTDRSFPDAFAELETD
                     EFIEARRECHQSMGRSATTPELDACAFDVVASGEPTYADAYAEVVEFRDEQRSAEVAP
                     LPDRPTTTRSGGATAPTGTAALTLEGKLAPISDPEPPADAVPQLEGEVDVPDGSLILV
                     SLECPAATDYVVTVSGPAGATTTLPLCGTQLGVPDDNDEPRSGEAYQWVADGGTHEIT
                     LEDLSPDGTTRRVELSVFVDDSPTADEYSEASWSGSLSGIGDVATLRI"
BASE COUNT          466 a         1097 c          948 g          375 t
ORIGIN      
        1 gccgttggag aggagggcta caccgacatc gccgcgcccg agtggaagcc ggcgaccgcc
       61 gccgaggacc tcgcctggcg ggaggagacc gacggcctcg acgcacagat cgccgtcgac
      121 gcgttcgatc tcgccttcgg cgacatgccc ggcgcgaccg agcgcgagct cgacgagccc
      181 ggctcggtgt cggccacccg ggcgtggggg ctcgtcgagc acttcgccga cgacctcacc
      241 cccgagcaga cgcgccgcgt cgccgaggtc cgcgccgagc gcggcacggg caccccgctc
      301 cccgacgccg cgggcgagct cccgccggag tgggaggcgc tcgccgacgg ggcgaccacc
      361 cccggggcga cggccccgcc gagcgagaac gcgccgcccc ccggagcgcc ggcgccgggg
      421 gcggcagccc caccgagcga cagcgcgacc accaccacgg cgcggacctc ctcgctgcgg
      481 aacctgcggg cgcccgctgc ggccgggttc gcccgcaagg cggcgcccga cgcagcgctg
      541 cagacgatcc tcaacgggct catgacccgg atcatcatcg gatggcggaa ccggctcggg
      601 aacgtcgggc tccccagcgt gagctcgtcg tactcgaaca ccggcctgac gaccgccacc
      661 ggcctcgacg cctacgccac cgccacgccg aacggcgacg gctgcctgat caccatctac
      721 ccgctgttgt acgagcacat cgtcaagctc actcccgcct acgtggcgtt cgtgctcgcc
      781 cacgagttgt tccactgcgt cgagttcgag tgggcgcccg gcttctggat gaccgcaccc
      841 gactgggtcg tcgagggcgg tgccgacttc gccgggtacg actactaccg caagcaggtc
      901 aaggtcgacg accagttcat cgagtggttc acgtcccgca acaccccgtt ggccagccgc
      961 tcctacagcg gagcgggcct gtgggagatc gcggcggccg aaggcatcga tccctactcg
     1021 tccatcaagg tcgcgctccg tgcgacgggc aacaccgaga ccgtgctggc cgcggccgga
     1081 ttgaactcac cgagtctgct gctcgactgg ccgagccgga ccgttcggtc gcaggcgtac
     1141 acccacccgt cgtggacgat gccgtggacc ggcgccggtg acggccgcca cgagaacttc
     1201 gagaagggcg ccaccatcac cctcggcggc acgaacgtgc gaggccccgc caactactcc
     1261 cacgccctgc aagcgcacgt cttcggaccg ggcgtcgaca tcgtgaccgt caggaacaag
     1321 ggcaagcacc tcgtcagcct ggccaacgac cgcgagaacc tcgtcctcgg gagcaacgag
     1381 accatgcgcc tgtgtgtcga cgagtcacgg tgcacgtgtc ccgacggcaa ctacggcagt
     1441 ctgcggttct tcaattcgac cgacgtcgcc gtgggcttct cggcgtcccg cgaagccggg
     1501 ctggtcacgg tcctcgcgga gaagttcgac aagaagcgtc actgcgagaa gcagcggccc
     1561 cgcggatcgg gcgacggcga cccgcacctg ctcacgatgg acgggatcgc gttcgacgtg
     1621 atgtcggtcg gcgagttcgt actggcgtcg acccccgaag ccgacgacac cccggcgttc
     1681 gacctgcaga tgcgcaccgc cgccgcggcg acggccggcg accggtactc gctcatcact
     1741 gcactcgccg tcgactacgg cgacgaccgg atcaccctca ccgcggccga ctacctctcc
     1801 aaacccgacg agatcgtggt gcgccacaac ggcgacaccg ccgacatcga cggcggaacg
     1861 ctcggggcgt tcgtcctcac tcgtgacgac gaacgcaact ggacgctctc cgcgcccgac
     1921 ggccccgagc tccggctgaa gtacctcaac gggttcttcg tggacgtcgc accgcccgag
     1981 acgaccgccg cccagcaggt cggcatgttg ggctctgcca acgacgaccc gctcgatgac
     2041 gtgcggcgag cggacggcac ccccgtcaac gccttcgacc ccgtcgaggt ccacggggcg
     2101 ttcgccgatt cgtggcgggt cacacccggg acgagccggt tcgactatct ccccggagag
     2161 tccaccgaca ccttcacgga ccggtccttc cccgacgcgt tcgccgagct cgagaccgac
     2221 gagttcatcg aggcccgccg tgagtgccac cagtcgatgg gccgatcggc cacgacgccc
     2281 gagctcgacg catgcgcgtt cgacgtcgtc gcatcgggcg agccgaccta cgccgacgcc
     2341 tacgccgagg tcgtcgagtt ccgcgacgag cagcgttccg ccgaggtggc accgctcccc
     2401 gaccgaccga ccaccacgag gtccggcgga gcgaccgcgc ccaccgggac cgcggcgctc
     2461 acgctcgagg gaaagctcgc cccgatctcc gatcccgaac cgcccgccga cgccgtgcca
     2521 cagctcgagg gcgaggtcga cgtgccggac ggctcgctca tcctcgtgtc gttggagtgt
     2581 ccggccgcca ccgactacgt cgtgaccgtg agcggcccgg ccggagccac caccaccctc
     2641 ccgttgtgcg ggacccagtt gggtgtgccc gacgacaacg acgagccccg ctccggggag
     2701 gcctaccaat gggtggccga cgggggcacc cacgagatca ccctggagga cctgtccccc
     2761 gacggcacca cgcgccgggt cgagctgtcg gtgttcgtcg acgactcgcc gactgccgac
     2821 gagtactccg aggcgagctg gagcggatcg ctctcgggta tcggcgacgt ggcgaccctc
     2881 cggatc
//
LOCUS       DAOSOA010000380         2836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_35358_length_2836_cov_5.629630, whole genome shotgun sequence.
ACCESSION   DAOSOA010000380 DAOSOA010000000
VERSION     DAOSOA010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35358_length_2836_cov_5.629630"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKY13_16055"
     CDS             complement(<1..182)
                     /locus_tag="PKY13_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT81508.1"
                     /translation="MPQQVRGVIAKAKGEPVSIETIVIPDPGPGEVVVAIAACGVCHT
                     DLHYREGGINDEFPFLL"
     gene            complement(243..2165)
                     /locus_tag="PKY13_16060"
     CDS             complement(243..2165)
                     /locus_tag="PKY13_16060"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOT81509.1"
                     /translation="MSRLRSAFLLVAAGLLLTLAVGHPAAAQSGFERIDSFDSTIVIN
                     KDGSADVTEVIAYNFGSTSRHGIERFIPTVFDWTGEAPKGSLPGARFDRVTPLENISV
                     SASPGTPDDVDTSYEQIGGVENTKIRIGDPDTTITGQHTYTIRYRLEGVLNGFADHDE
                     LYLNITGNGWSVPIGSTTATVSAPGAISRVTCFAGPVKSVLPCDSSTSTESATAGQAT
                     FSQGALGSGDGLTVVVGLPKGVVTDPAKTRILRERWSIGSAFRLNGATGAASGGMLVA
                     GLGLVGMLGWRNGRDRRYAGSVVDAAFGNATGEDGPVPLFDKTVHTVEFVPPEGIRPG
                     HMGTLWDEQANHLDVSAMIVDLAVRGWLRIDEIAPQSDGFLGFGGDGGDFQLVQLRDH
                     TTDPQASELWEAERSLLASLFATGSTVQLSDLKTKFADKLSIIEGHLYDDAVAAGWFP
                     ARPDKIRSRWHGRGFVLLLLGIGLTVVVAKFTHFGLVALPVPLLGLVLLGSAKYFPRR
                     TAKGSALLGRVRGFKELFDAGEGERQRFAEAHHLFAQYLPYAIVFGCTDKWAEVFAGL
                     GLTPQEMGLGVWYTSPYGYDPIHFGYAMGSFTTHSTGSLAAAAPSSSGGASSGGSGFS
                     GGFSGGGFGGGGGGSW"
     gene            2208..2765
                     /locus_tag="PKY13_16065"
     CDS             2208..2765
                     /locus_tag="PKY13_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT81510.1"
                     /translation="MVATPSAMLPLGTQAPDFELPDPFGALQTLEDVGGESPVLVVAF
                     LSNHCPYVKHIGREIGLVTQRLIARGVAVVGIMSNDVDAYPADAPPLMGSTAASYGWD
                     FPYLYDETQDVARAYHAACTPDFFVFDADRRLAYRGQFDGARPSNDVGATGADLKAAV
                     NALLEGRRPTDDQLPSLGCNIKWRG"
BASE COUNT          465 a         1010 c          939 g          422 t
ORIGIN      
        1 agcaggaacg ggaactcgtc gttgatgccg ccctcgcggt agtgcaggtc ggtgtggcac
       61 acaccgcagg ccgcgatcgc cacgacgacc tcgccggggc cgggatcggg gatgacgatc
      121 gtctcgatcg acaccggctc gcccttggcc ttcgcgatca ctccacgcac ctgctgcggc
      181 atctccaccc cttcgatcaa acggtcggcg tcatgctgcc acagctgtcc gccactcgtt
      241 ggctaccagg agccgccgcc accaccgccg aagccaccac cgctgaaccc gccgctgaag
      301 ccgctgccac ccgacgaggc accaccactc gaggacggcg ccgcggcggc cagcgagccg
      361 gtcgaatggg tggtgaagga ccccatggcg tacccgaagt ggatcgggtc gtagccgtac
      421 gggctcgtgt accacacccc gaggcccatc tcctggggag tcaacccgag accggcgaac
      481 acctcggccc acttgtcggt acagccgaac acgatcgcgt acgggaggta ctgggcaaag
      541 aggtggtgtg cctcggcgaa ccgctggcgc tcaccttctc ccgcatcgaa cagctccttg
      601 aacccgcgga cgcggccgag gagcgccgag cccttcgccg tgcgccgcgg gaagtacttc
      661 gcactgccga gcagcacgag tccgagcagg ggaacgggca gggcgacgag tccgaagtgg
      721 gtgaacttcg ccaccaccac cgtcaggccg atgccgagga gcaggagcac gaacccccgt
      781 ccgtgccacc ggctccgaat cttgtcgggc cgcgccggga accatccagc ggcgacggcg
      841 tcgtcgtaga ggtgcccctc gatgatcgag agcttgtcgg cgaacttggt cttgagatcg
      901 gacagctgca ccgtcgagcc cgtggcgaac agtgacgcaa gcagggaccg ctctgcctcc
      961 cacaactcgc tcgcctgcgg atcggtcgtg tggtcgcgca gttgcaccag ctggaagtcg
     1021 ccgccgtccc caccgaatcc gaggaacccg tcggactgcg gagcgatctc gtcgatgcgg
     1081 agccagcccc gcaccgcgag gtcgacgatc atcgccgaca cgtcgaggtg gttcgcctgc
     1141 tcgtcccaca gcgtgcccat gtggccggga cgtattcctt cgggcggcac gaactcgacg
     1201 gtgtgcaccg tcttgtcgaa cagcggcacg gggccgtcct cgccggtcgc attcccgaac
     1261 gcggcatcga cgacgctgcc ggcgtaccga cggtcgcgac cgttgcgcca acccagcatg
     1321 ccgaccagac cgaggccggc gacgagcatg ccgcccgagg ccgcgcccgt cgcgccattg
     1381 agacggaacg ccgagccgat cgaccagcgt tcccgcagga tgcgggtctt cgccgggtcg
     1441 gtcaccactc ccttgggaag gccgaccacc acggtcagcc cgtcgccgct gccgagcgcg
     1501 ccctgggaga acgtcgcctg ccccgccgtg gcgctctcgg tcgaggtcga cgagtcgcac
     1561 gggagtaccg acttcacggg accagcgaaa cacgtgaccc gcgagatggc gcccggggcg
     1621 ctcaccgtcg ccgtcgtcga gccgatcggg actgaccagc cgttgcccgt gatgttgagg
     1681 tacagctcgt cgtggtcggc gaagccgttg aggacaccct cgaggcggta acggatcgtg
     1741 taggtgtgct ggccggtgat cgtcgtgtcc gggtcgccga tgcggatctt cgtgttctcg
     1801 acgccaccga tctgctcgta gctggtgtcg acgtcgtcag gggtgccggg gctcgccgag
     1861 accgagatgt tctcgagcgg ggtcacccgg tcgaagcggg cgccggggag cgaccccttg
     1921 ggcgcctcgc cggtccagtc gaagaccgtg gggatgaacc gctcgatgcc gtgccggctc
     1981 gtcgacccga agttgtaggc gatcacttcg gtgacgtccg ccgaaccgtc cttgttgatc
     2041 acgatggtcg agtcgaacga gtcgatccgt tcgaacccgg actgcgcagc ggccggatgg
     2101 ccgacggcca gggtgagcag cagcccggcg gcgaccagga ggaaggcgga acgcagtcgg
     2161 ctcatgtcga cgtcaggcta cctgtgcccc tcggctaccg tgcggcgatg gtcgccactc
     2221 cctccgccat gctcccgctc ggcacgcaag cgccggactt cgagctcccc gatcccttcg
     2281 gcgccctcca gacactcgaa gatgttggcg gcgagagccc ggtgctcgtc gtggcgttcc
     2341 tctcgaacca ctgtccgtac gtgaagcaca tcggacgcga gatcggcctc gtcacccagc
     2401 gactcatcgc ccgcggcgtc gccgtcgtcg gcatcatgtc gaacgacgtc gacgcgtatc
     2461 ccgccgatgc gccgccgctc atggggagca ctgcggcctc ctacggctgg gacttcccgt
     2521 atctgtacga cgagacccag gacgtcgccc gcgcctacca cgcggcctgc acccccgact
     2581 tcttcgtgtt cgacgccgac cgtcgcctcg cctaccgcgg ccagttcgac ggggcccggc
     2641 cgtccaacga cgtcggggcc acgggcgccg acctgaaggc cgcggtgaac gcgttgctcg
     2701 agggccgtcg gcccaccgac gaccagctcc cctcgctcgg ctgcaacatc aagtggcggg
     2761 gatgaagcgc aactcgccga cgatgcaagg tccacccgac cgaggtaccg gagcgatcag
     2821 tagggagcgc agcgac
//
LOCUS       DAOSOA010000381         2832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_35417_length_2832_cov_7.335254, whole genome shotgun sequence.
ACCESSION   DAOSOA010000381 DAOSOA010000000
VERSION     DAOSOA010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35417_length_2832_cov_7.335254"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..787)
                     /locus_tag="PKY13_16070"
     CDS             complement(5..787)
                     /locus_tag="PKY13_16070"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT81511.1"
                     /translation="MSWTDGLAGGEPPPPAGPPANVPWGPPTPAGPPTSTWGAPTTAW
                     GAPAPAWGVPAPTAPRGSVYAEYGVHDVPVSYAGFWRRLAGAFIDGLVLGLINIPVNL
                     VFQLVGMAFVSATRSSALLVVLLLANVCVNLFIWWRFVPGRIGSTGATVGMSAMHIKL
                     VPASGRGGVSSGLAFVRALLASVLQFVFLIPAFLYGLTRLVGTDLEAPDSFRTHVVPN
                     AFVASVFVFLVLLVYLPSLWNLFDRRYQTLYDKLCGVVVIND"
     gene            complement(833..1981)
                     /locus_tag="PKY13_16075"
     CDS             complement(833..1981)
                     /locus_tag="PKY13_16075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT81512.1"
                     /translation="MSTDVVIVSGVRTPIATAYKGSLGGVDAFALAELVIKEAVERSG
                     IAPADFEDMGFGESFQGGGNIGRNAAIRAGLNNLPGVATQRWCASGMAGVQWVAANIS
                     AGMIDAGLAGGVESMTTAPASSKGEESFWLSPANEPTELAPPFNTALGVGDNAARIAG
                     ISREDADLWAFTSHQNAIRAIDEGRFKNEIVPVPLPGGGEFSVDEHPRRGSTLEKLAS
                     LGVLNPQYDWATTTAGNASGLNDAAAALTIVSRAYAEAHGLKPLAVIRGWASVALTPE
                     ETGLTPEPAMRKALAKAGIGVSDLQAVEINEAFASVAVAATRRLGLDEGIVNQNGSGC
                     SLGHPIGCTGARMIVTMLNELERTDSQWGAVAMCAAGGMGSATVIERV"
     gene            2075..>2832
                     /locus_tag="PKY13_16080"
     CDS             2075..>2832
                     /locus_tag="PKY13_16080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOT81513.1"
                     /translation="MKLDTQLFGLGEAQARAAVLAELGLDGVFTFEGPHDVFTPLVLA
                     APGTSLDLYTNAAIALPRNPIQLAHQAWDLQALSEGRFALGLASQIRTQIEKRYGTDF
                     HPPLGRMRELVAAMRAIFDSWQNGSRLDFRGDYYTHTLMPPLFNPGPLEWGPPPIWLG
                     GLGPKMVEMAATVADGLLVMPFCTERFFTEHTLVAVDRGLAASQRSRADFSIVCESII
                     AVGRDEQELATATEGVRFLLSFYGSTPAYRPVLDA"
BASE COUNT          452 a          938 c         1025 g          417 t
ORIGIN      
        1 ccggtcagtc gttgatcacc acgactccgc acagcttgtc gtagagcgtc tggtaacggc
       61 ggtcgaacag gttccagagc gacggcaggt acacgaggag gacgaggaac acgaagaccg
      121 acgcgacgaa cgcgttgggc acgacgtggg tacggaacga gtcgggcgcc tccaggtcgg
      181 tgccgacgag tcgggtgagg ccgtacaaga acgcgggaat gaggaatacg aactggagga
      241 cggacgccaa cagcgcccga acgaacgcca agccgctcga caccccaccg cgaccgctgg
      301 cggggacgag cttgatgtgc atcgcgctca taccgacggt cgctccggtg ctgccgatgc
      361 ggccgggcac aaagcgccac cagatgaaga ggttgacgca cacgttcgcg agcaacagca
      421 cgacgagcag ggccgatgat cgggttgcgc tgacgaaggc catgccgacg agctggaaca
      481 cgagattgac cgggatgttg atcagcccca gcaccaggcc gtcgatgaac gcgccggcga
      541 ggcgccgcca gaacccggcg tagctgaccg gcacgtcgtg gacgccgtac tccgcgtaca
      601 ccgatccgcg aggtgcggtc ggcgccggta cgccccaggc gggcgctggc gcgccccagg
      661 ccgtggtcgg cgcgccccac gttgaggtcg gcggcccagc gggggtcggt ggaccccacg
      721 gcacgttggc gggtgggccg gccggaggcg gaggttcgcc accggccagc ccgtcagtcc
      781 acgacatgcc gcaaagagta ggtggagttg cccagcgagg gcggagtgga actcagacgc
      841 gttcgatgac ggtggcggag cccatgccgc cggccgcaca catcgcaacg gcaccccact
      901 gcgagtcagt gcgctcgagt tcgttgagca tcgtgacgat catgcgagcg ccggtgcagc
      961 cgatggggtg accgagcgag cagcccgagc cgttctggtt gacgatgccc tcgtcgaggc
     1021 cgaggcggcg ggtcgccgcc acggcgacgg acgcgaacgc ctcgttgatc tcgacggcct
     1081 ggaggtcgga cacgccgatg ccggccttgg cgagcgcctt gcgcatggcg ggctcggggg
     1141 tgaggcccgt ctcctcgggg gtgagggcca ccgatgccca gccgcggatc acggcgaggg
     1201 gcttgaggcc gtgggcctcg gcgtaggcgc gggacacgat cgtgagcgca gcggcggcgt
     1261 cgttcaggcc cgacgcgttg ccggctgtgg tcgtcgccca gtcgtactgc gggttgagca
     1321 caccgagcga ggccagcttc tcgagcgtgg agccacggcg ggggtgctcg tcgacggaga
     1381 actcgccgcc gccgggcagg ggcacgggca cgatctcgtt cttgaaacgg ccttcgtcga
     1441 tggcgcggat ggcgttctgg tgcgacgtga acgcccagag gtcggcgtcc tcgcggctga
     1501 taccggcgat gcgggcggcg ttgtcaccga cgccgagggc ggtgttgaac gggggagcga
     1561 gctcggtggg ctcgttggcg ggcgagagcc agaagctctc ctcgcccttc gagctggcgg
     1621 gagcggtcgt catcgactcg acaccaccgg cgaggccggc gtcgatcatc ccggccgaga
     1681 tgttggcggc gacccactgc acgccggcca tgcccgaggc gcaccagcgc tgggtggcga
     1741 cgcccgggag gttgttcagg ccggcgcgga tcgcggcgtt gcggccgatg ttgccgccgc
     1801 cctggaacga ttcgccgaag cccatgtcct cgaagtcggc gggggcgatg cccgaacgct
     1861 cgacggcttc cttgatcacg agctcggcga gggcgaaggc gtccacccca ccgagcgagc
     1921 ccttgtaggc ggtggcgatc ggcgtgcgaa cgccgctgac gatgacgaca tcggtggaca
     1981 ttgaggattc cccctgggat tctgacggtt cgtcagattg tgtcatgtag cgtcgcctgc
     2041 cgggaagccg gagacccggg aggtcggcac tgccgtgaag ctcgacacac agctgttcgg
     2101 tctcggggag gctcaggcgc gggcggcggt gctcgccgaa ctcggtctcg acggcgtctt
     2161 cacgttcgag gggccccacg atgtgttcac gccattggtc ctcgctgcgc cgggaacgtc
     2221 gctcgacctc tacaccaacg cggcgatcgc cctaccccgc aacccgatcc aactcgccca
     2281 ccaggcgtgg gacttgcagg cgctcagcga ggggcggttc gcgctcggcc tcgccagcca
     2341 gatccgtacc cagatcgaga agcggtacgg caccgacttc catccgcccc tcggtcgtat
     2401 gcgcgagctg gtggcggcga tgcgggcgat cttcgattcc tggcagaacg gcagtcgcct
     2461 cgacttccgc ggcgactact acacccacac gctgatgccg ccgctcttca accccggccc
     2521 cctcgagtgg ggcccgccgc ccatctggct cggcggcctc gggccgaaga tggtggagat
     2581 ggcggcaacg gtcgccgacg gcctcctcgt catgccgttc tgcaccgagc gattcttcac
     2641 cgagcacacc ctcgtcgccg tcgaccgtgg cctggcggcg tcccagcgtt cccgggccga
     2701 cttcagcatc gtgtgcgagt cgatcattgc ggtcgggcgt gacgagcagg agctcgccac
     2761 cgcgaccgag ggcgttcgct tcctgctcag cttctacggc tcgacgcccg cctatcgacc
     2821 ggtgctcgac gc
//
LOCUS       DAOSOA010000382         2832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_35422_length_2832_cov_4.355420, whole genome shotgun sequence.
ACCESSION   DAOSOA010000382 DAOSOA010000000
VERSION     DAOSOA010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35422_length_2832_cov_4.355420"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2156)
                     /locus_tag="PKY13_16085"
     CDS             complement(<1..2156)
                     /locus_tag="PKY13_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81514.1"
                     /translation="MLVRSTCRAAVARTVCAALLVAAVTVGCSDDDAPEDAAATTTTV
                     TAPRATPIDAEDAAQLATDVGDGSCDEIDTTRCLLPFPSNRFTEASDSTDTKLRVHLP
                     EGQLANASGSTLDVTEWNRNDGFSPGTPMLVSAPDVDLAASEAPPIGDIGQSMEESSP
                     TIVIDLDTGERLAHWVELDSNAPAGQQLLIVRPAAALPEGHHIGVVLGALVDSSGTPL
                     EASLGFRVYRDRLTTELDAVEDRRPDMEALFEAAAGLGADRSTLYAAWDFTVASERNL
                     SERLLTMRDDAFERLGTAAPRFTVNEVVTDDLPDGIGRRVIGTFDVPSYLTGAAEPGS
                     RLTGAEDDSVPSYAGYDYAANFTCQIPTVALDGAGGTTRPVVYGHGLLGGAGEAENSQ
                     VAKIASTNDMMYCATDWIGMSEGDVGNAVAILNDLSKFPSLPDRSQQGILNSLFLARV
                     MKADGGFSSNEAFLNTGGTSIIDRAEVYYDGNSQGGIMGGAATAVSTEWTKAVLGVPG
                     MDYALLLSRSVDFNTYFNVLRGAYPDPIDQALIYPILSMLWDRGETNGYAQHMTDDPY
                     DGTPEHQVLLHVGFGDHQVATVAAEMEARTIGAVVRGPALADGRHPDAKPFYGLQVQT
                     EFPTDKSVLVYWDSGTLPPPPGNITPAASPEFAAACSTLSGDDRERDTECADSHEDPR
                     RAPESILQKDLFLRPDGRIEDTCNAQPCTAVHRKLL"
     gene            complement(2231..>2832)
                     /locus_tag="PKY13_16090"
     CDS             complement(2231..>2832)
                     /locus_tag="PKY13_16090"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HOT81515.1"
                     /translation="NDPKLVALSEQREALASKVADALDAVFQAGATEADAERQVAQAA
                     ADEAEAAAAAAAQARADAERTAKAAKESRNAPAATAPPARAGAVPAATPAATPAVGGG
                     IVILTEPSAPLPDLPDGGPSAEAWAEVRRCESGGNYRAVSRSGRYRGAYQFDMQTWAA
                     MGGKGDPAGALPLEQDARARLLFNQRGARAWPQCGRVLL"
BASE COUNT          393 a          968 c         1011 g          460 t
ORIGIN      
        1 tcgaggagct tgcggtgcac cgcggtgcac ggttgcgcgt tgcacgtgtc ctcgatacga
       61 ccgtcgggcc ggaggaacag gtccttctgg aggatcgact cgggcgcgcg acgggggtcc
      121 tcgtgcgaat cggcgcactc ggtgtcccgc tcacggtcgt ccccggagag cgtcgaacag
      181 gcggcagcga actccggtga cgcggccggc gtgatgttgc ccggtggcgg aggaagggtg
      241 ccgctgtccc agtagacgag caccgacttg tcggtcggga actcggtttg cacctgcagc
      301 ccgtagaacg gcttggcatc ggggtggcgg ccgtcggcca gcgccggacc tcgaacgacc
      361 gcgccgatcg tccgcgcttc catctcggcg gcaacggtcg cgacctgatg gtccccgaag
      421 ccgacgtgga ggagcacctg gtgttccggt gtgccgtcgt aggggtcgtc ggtcatgtgc
      481 tgtgcgtagc cgttggtctc gccgcggtcc cagagcatcg acaggatcgg gtagatcagt
      541 gcttggtcga tggggtcggg atacgcaccg cggagcacgt tgaagtacgt gttgaagtcg
      601 acgctgcggg agagcagcaa cgcgtagtcc atgcccggga caccgagcac cgccttggtc
      661 cactcggtcg agaccgccgt ggcagcgccg cccatgatgc cgccctggct gttgccgtcg
      721 tagtacacct cggcgcggtc gatgatgctc gttccgcccg tgttgaggaa tgcctcgttc
      781 gacgagaacc ctccgtcggc cttcatcacc cgcgccagga acagggagtt gaggatgccc
      841 tgctggctgc gatcggggag gctggggaac ttcgacaggt cgttgaggat ggcgaccgcg
      901 ttgccgacgt cgccctcgct catgccgatc cagtcggtgg cgcagtacat catgtcgttg
      961 gtcgatgcga tcttcgcgac ctgcgagttc tccgcctcgc cggcgccacc gagcagaccg
     1021 tgtccgtaca cgaccggccg cgtggtgccg cccgccccgt cgagcgcgac ggtcgggatc
     1081 tggcacgtga agttcgcggc gtagtcgtac ccggcgtaac tcggaacgct gtcgtcctcg
     1141 gcgccggtca agcgggaacc cggctcggcc gcgcccgtga ggtacgacgg cacgtcgaag
     1201 gtgccgatga cccggcgacc gatgccgtcg gggaggtcgt cggtgaccac ctcgttcacg
     1261 gtgaaccgcg gtgcggccgt gccgaggcgt tcgaacgcgt cgtcccgcat cgtcaacaga
     1321 cgttccgaca ggtttcgctc cgaggcaacc gtgaagtccc aggcggcgta cagggtcgaa
     1381 cgatccgcac cgagcccggc cgctgcctcg aacagcgcct ccatgtccgg ccgccgatcc
     1441 tcgaccgcgt cgagctcggt ggtcaggcga tcgcggtaca cgcggaaccc gagggacgct
     1501 tcgagcggtg tccccgacga gtcgaccagc gcgcccagca caaccccgat gtggtgcccc
     1561 tcgggcagcg ctgcggcggg ccggacgatc agcaactgct gtcccgccgg cgcgttggaa
     1621 tcgagctcga cccagtgggc gagccgttcg ccggtatcga ggtcgatgac gatcgtgggg
     1681 gacgactcct ccatcgactg accgatgtcc ccgatcggcg gggcctcact ggcggccagg
     1741 tcgacgtcgg gagcgctcac caacatgggg gtaccggggc tgaagccgtc gttgcggttc
     1801 cactccgtca cgtcgagcgt gctcccgctg gcgttggcca gctgtccctc gggcagatgg
     1861 acccgcagct tcgtgtcggt cgaatcgctc gcttcggtga accggttgct cgggaagggc
     1921 agcaggcacc gcgtggtgtc gatctcgtcg cacgaaccgt cgccgacatc ggtggcgagt
     1981 tgtgccgcgt cctccgcatc gatcggcgtc gcccgcggag ccgtgaccgt ggtggtcgtg
     2041 gcagcggcgt cctcgggagc gtcgtcgtcg gagcacccga cggtcaccgc ggcgaccagg
     2101 agggcggcgc acaccgtccg ggccacggcc gcccggcacg tcgatcgaac cagcactctc
     2161 gccatcgaat ccccccggat cctcgacggc gatcgctgag accgccggtg aacggcccga
     2221 gactacgtgc ctagagaagc actcggccgc attgcggcca ggcccgggca ccacgctggt
     2281 tgaacagcag ccgggcacgg gcatcctgtt cgagtggcag cgccccggcg gggtcgccct
     2341 tgccgcccat cgcggcccac gtctgcatgt cgaactgata cgcaccgcga tagcgaccgc
     2401 tgcgactgac ggcgcggtaa ttgccgccgg actcgcaacg ccgtacctct gcccacgcct
     2461 cggccgaggg gccgccgtcg ggcaggtcgg gcagcggcgc cgacggttcg gtgaggatca
     2521 cgatgcctcc gcccactgcc ggcgtggccg ccggggtcgc ggccggcacc gcaccggccc
     2581 gggcgggcgg cgccgtggcg gcgggggcgt tgcgggactc cttcgcagcc ttcgccgttc
     2641 gctcggcatc ggcacgggct tgcgctgcgg cagccgccgc ggcctccgcc tcgtctgctg
     2701 ccgcttgggc gacctgccgt tcggcgtccg cctcggtcgc tccggcctgg aacacggcgt
     2761 cgagggcgtc ggcgactttg ctggccagcg cctctcgctg ttcggacagt gcaacgagct
     2821 tcgggtcgtt gt
//
LOCUS       DAOSOA010000383         2829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_35464_length_2829_cov_5.019466, whole genome shotgun sequence.
ACCESSION   DAOSOA010000383 DAOSOA010000000
VERSION     DAOSOA010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35464_length_2829_cov_5.019466"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1201)
                     /locus_tag="PKY13_16095"
                     /pseudo
     CDS             complement(1..1201)
                     /locus_tag="PKY13_16095"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            1365..1640
                     /locus_tag="PKY13_16100"
     CDS             1365..1640
                     /locus_tag="PKY13_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOT81516.1"
                     /translation="MPNFKVWIDQDLCTGDGICEEITPALFSGHDDGLWYVKEKEWAD
                     IYNGGPKDAPVYRMAEGQATVPENLVESAIESAEECPGECIFIEVLD"
     gene            1613..1981
                     /locus_tag="PKY13_16105"
     CDS             1613..1981
                     /locus_tag="PKY13_16105"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOT81517.1"
                     /translation="MHLHRGARLSSAPDDSALRAERERLTGIVASLRAAFEDIVDAAA
                     DVATDDEHDPEGHTIAFERQQQAALLRETEAQIDEVDRALERMGAGSYGRCETCGAPI
                     PAERLDAVPATRFCVTCAAV"
     gene            complement(2031..>2829)
                     /locus_tag="PKY13_16110"
     CDS             complement(2031..>2829)
                     /locus_tag="PKY13_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81518.1"
                     /translation="GQREGREQYAYDALIDLTTGANGSTEPGDTSDADTDAGGTDDSV
                     RRSGQGGSSGRGSRRRESVRRLMLVRVDLAALVNGAVGDGDVCEIPGIGPVSVEQART
                     MFGDSILRLVTTNGTAVIDVVNPARKPTEAQRIAKLWTDPKCCVAGCNRTVDLEYEHR
                     EDYARTQHTVLVESEHMCTFHHQLKTLENWQLVAGTGRRAFVDPDDPRHPHHIPDLTT
                     GSTTDPPEAPTRAPEPPEPPGRPRAAPASPELRARYDAAHRAARRAE"
BASE COUNT          427 a          947 c         1006 g          449 t
ORIGIN      
        1 acttgcgggt gcggtcgagc acgtgcacct cgcggcccca caccccggcg agcgcccact
       61 cccgaacgct cgtcgccgag ccgtcgagca acggccgcag cgacgtcccg tgtgcccggg
      121 tcggcgcatc gacgccgaac gcctcgcaca aggtggcgtg caggtcgacg ttggtggtga
      181 gcgcgtcgac cgtactcccg ggcaccgaca ccccgggcca cgagatgaac agtggtgtgt
      241 gcagcagcgg ctcgaaaagc gggatgccgg gcttgcccca cgcatctcgc tcgcccaggt
      301 agtggccgtg gtcggtgcac acgatcacga cggtgtcgtc ccaccagttg ccggcgtcga
      361 gctggtcgag cacccgaccc aaccagtggt cgatcatcga gagcttggcg ccgtactggg
      421 cgcggatctg ccggccctgc cgttcggaga tcgccccgct cccaacgccc gacccggtgt
      481 acggcggcca gaccaaatgg ggcccatcta cgcctgcgtc ccactccgcg tcgtagcgcg
      541 acgcccaggg ttcgggagtg tcgaagggtt cgtgcgggtc gaactcgtcg acgaacagca
      601 gccacgggac cccggcgtcg cgatggaacg gagcatggtc gcgcagccac gacgccgccg
      661 ccgtcatcgt ccgcgggccg gggaagtcgg cctcgtcgcg gaaccagccc cgcgagttgt
      721 cgtacggggt gtgcccacgg ccgaagctcg gagcaccggc ccacgaggga tcgggacggg
      781 tcttccacgt gtcgccctcg tgccctcgct ggtagtccca ggccgtgaac tcggtgtggt
      841 agttctcgcc gccggtctcg aaaagatgag ggtggtcggt gaccagctgg gtcgtgaccc
      901 cggcgcgccg gagcgttgcc gtcaccggtt cgtcccacag ctccaacgat ccccacgggc
      961 gctacaggaa gtcgagcgaa ccggtgagca ggtcgtgacg ggccggcatg cacggcagcg
     1021 acccggcatg gtggttggtg aaccgggtcg aacgtcgggc cacgcggtcg aggttcgggg
     1081 tgtcgaactc ctcggacccg taggagccca ggagatgacg gttgagcgag tccaggagga
     1141 tgacgacgac gttctgcgct cggtcacggt cctctcgagc cccaccggcg gtttcattca
     1201 tgggccagtg atctaccagg aacggcgggc cgatgcgctc ggccgcatgc gatgtcgacg
     1261 ttccactgac aggtcggtcg cgaccgctgt tcgtcgtgac cgctcgcgag gccgtcgacc
     1321 tgtgagactg cggtccacgt ccccgaacgc gaaagcgagc cgacatgccc aacttcaagg
     1381 tctggatcga tcaggacctg tgcaccggtg acggcatctg cgaggagatc acccccgcgc
     1441 tcttcagcgg ccacgacgac ggtctgtggt acgtgaagga gaaggagtgg gccgacatct
     1501 acaacggcgg cccgaaggac gcacccgtgt accgcatggc cgagggtcag gcgacggtcc
     1561 ccgagaacct ggtcgagtcg gcgatcgagt cggccgagga gtgccccggc gagtgcatct
     1621 tcatcgaggt gctcgactga gttcggcgcc cgacgacagc gcgctccgcg ctgagcgaga
     1681 gcgactgacc ggaatcgtcg cgtcgctccg agccgcgttc gaggacatcg tcgacgccgc
     1741 cgccgatgtc gccaccgacg acgagcacga tcccgaaggc cacacgatcg cgttcgaacg
     1801 acagcagcag gccgcgctcc tccgcgagac cgaagcacag atcgacgagg tcgaccgagc
     1861 cctcgagcgg atgggcgccg gatcgtacgg gcggtgcgag acctgcggcg cgcccatccc
     1921 cgccgaacga ctcgacgccg tccccgccac ccgcttctgc gtgacctgcg ccgcagtctg
     1981 acggcatccc gtccggccgg tgcggccggt gcgagcagtt tccacagcga tcactccgcg
     2041 cggcgcgcgg cgcgatgcgc tgcgtcgtac cgggcgcgaa gttcaggtga cgcgggcgcg
     2101 gctcgtggtc ggccgggcgg ttcgggcggc tcgggcgctc gtgtcggggc ctcgggtggg
     2161 tcggtggtgc tgccggtggt gaggtcgggg atgtgatggg gatggcgggg atcgtcgggg
     2221 tcgacgaacg ctcggcgccc ggtgccggcg acgagttgcc agttctccag ggttttgagt
     2281 tggtggtgga acgtgcacat gtgttcggac tcgaccagca cggtgtgctg ggttctcgcg
     2341 tagtcctcgc gatgctcgta ctcgagatcc accgtccggt tgcacccggc gacgcagcat
     2401 ttcgggtcgg tccagagttt ggcgatgcgt tgtgcttcgg tcggtttgcg tgcggggttc
     2461 acgacgtcga tgacggcggt gccgttcgtg gtgaccagcc gcaggatcga gtcgccgaac
     2521 atggtccggg cttgttcgac cgacaccgga ccgatgcccg ggatctcaca cacgtcaccg
     2581 tcacccaccg ccccgttgac gagggcagcg aggtcgactc ggaccagcat caggcggcgc
     2641 accgattcac ggcgccgtga cccgcggccg ctggacccac cctgcccgct tcggcggacg
     2701 ctgtcatcgg tcccgccagc gtcggtatcg gcatcgctcg tgtcgccggg ttcggtgctc
     2761 ccgttggcac cggtggtgag gtcgatcagc gcgtcgtagg cgtactgctc gcggccttca
     2821 cgctgcccg
//
LOCUS       DAOSOA010000384         2815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_35691_length_2815_cov_6.675725, whole genome shotgun sequence.
ACCESSION   DAOSOA010000384 DAOSOA010000000
VERSION     DAOSOA010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35691_length_2815_cov_6.675725"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1011
                     /gene="rarD"
                     /locus_tag="PKY13_16115"
     CDS             73..1011
                     /gene="rarD"
                     /locus_tag="PKY13_16115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOT81519.1"
                     /translation="MPPTPSPERSDHRLGILAGMGSYALWGLFPLYFHSLEPAKPLEV
                     LAHRIVWSFVLMLIVLAVQRNWGWIRELRADPRRAGFIVLAGVLLSVNWLVYVWAVSV
                     DRVIDASLGYFVNPIVTVALGVVLLKEKIRPVQVGALAFGAAAVVILAVAYGQVPWIA
                     LTLAFSFAGYGYIKKQVGLDTVASLTAETALLFPIAAGGLVWAFVTDRTAIGHHGAFL
                     DVKLLALGAVTAVPLLLFGVATRRIPLVMIGLLQYLTPWGQLLLGWLYFDEKMPPSRL
                     AGFALIWVALALLAYDGVQSVRQSRNEAVAVEPELV"
     gene            complement(1090..2127)
                     /locus_tag="PKY13_16120"
     CDS             complement(1090..2127)
                     /locus_tag="PKY13_16120"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HOT81520.1"
                     /translation="MQQVVITKVGGPEVLQVREAPDPSPMRGEVRIAVAAAGLNFADV
                     SARVGLYPDAPKPPMTVGYEVSGTVDAVGGGVEGFAVGDRVVSLTRFGGQSSSVVVPA
                     HQAVHLPDGLDLVHAAAIPVNYLTAYLMLVRLGNVRKGESVLIHAAAGGVGFAALQIC
                     RLLGAVTYGTASSGKHERLRDAGLDHPIDYRNQDFAAEVNRLTKGRGVDVILDAVGGR
                     TSRKNYQLLRPLGRLFLFGASAASQDQKRNILKAGAAIAQFPVFHPLALMNANKGVFG
                     VNLGHLWDEKEMMSEVLTELLGWWSEGKIEPVVDSVFPFSKAADAHDQLQLARNVGKV
                     LLVPDGLGHLR"
     gene            2168..>2815
                     /locus_tag="PKY13_16125"
     CDS             2168..>2815
                     /locus_tag="PKY13_16125"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT81521.1"
                     /translation="MARPSVTPRGLAVARGDEPADLLIRGGRVFVSSTRSWIETSLAI
                     ADGCIVGWGDREALDVVDVDGAALTPAFVDAHMHLESTKLWVDEFVRAVLPHGTTAVA
                     ADPHEIANVFGIPGVAALIAAAADLPFHFGVCASSCVPASPFESAGAELSHTDVADLL
                     DGHGAIGVAEVMNFPGVIGGDPEMLARIATAGSRRVDGHAPGLRGQQLDAYLAAGV"
BASE COUNT          407 a         1002 c          951 g          455 t
ORIGIN      
        1 cagcgttgct actcgtttcc atcaggaata ctcacgaagg gaaggagttc ccgacgacgt
       61 aaggtcgccg gagtgcctcc gaccccctca cccgaacgct ccgaccaccg cctcggcatc
      121 ctcgcgggca tgggcagtta cgccctgtgg ggcctgttcc cgctgtactt ccactcgctc
      181 gaaccggcga agccgctcga ggtgctggcc caccgcatcg tctggtcgtt cgtgctcatg
      241 ctcatcgtgc tggcggtgca gcggaactgg ggatggatac gcgaacttcg cgccgacccc
      301 cggcgggcag ggttcatcgt gctggccggt gtgctgctca gcgtcaactg gctcgtgtac
      361 gtgtgggcgg tgagcgtcga tcgcgtcatc gacgcgtcgc tcggctactt cgtcaacccg
      421 atcgtgacgg tcgccctggg cgtggtcctg ctcaaggaga agatccgccc cgtgcaggtc
      481 ggggcgttgg cgttcggcgc tgctgccgtc gtcatcctcg ccgtcgcgta cgggcaggtc
      541 ccgtggatcg ccctcacgct ggcgttcagc ttcgccggct acggctacat caagaagcag
      601 gtcggcctcg acacggtcgc gtcgctcacc gccgagacgg cgctgctctt cccgatcgcc
      661 gccggtggac tcgtctgggc gttcgtgacc gaccgcacgg cgatcggtca ccacggcgcg
      721 ttcctcgacg tgaaactcct cgcgctgggc gccgtgaccg cggtgccgct cctgctcttc
      781 ggggtggcga cccggcgcat ccccctcgtg atgatcggcc tgctgcagta cctcacgccg
      841 tggggccagc tgctcctcgg ctggctgtac ttcgacgaga agatgccgcc gtcacggctt
      901 gccggcttcg ccctcatctg ggtcgcgctg gccctcctgg cgtacgacgg cgtgcaatcg
      961 gtgcggcagt cccgcaacga ggccgtggcc gtcgagcccg aactcgtctg acggtcaggc
     1021 gtcaggcatc cggtacgagc cgaggtaccg aagcgtcaga gaagctcccg acggagtcgg
     1081 gagcgaacct cagcgcaggt ggccgaggcc atcaggaaca agcagcacct tgcccacatt
     1141 gcgggcgagc tggagctgat cgtgggcgtc ggccgccttg ctgaacggga acaccgagtc
     1201 gacgaccggc tcgatcttgc cctccgacca ccaccccaac agctcggtga gcacctcgct
     1261 catcatctcc ttctcgtccc acaggtgccc gagattgacg ccgaacactc ccttgttggc
     1321 gttcatcagg gccaacggat ggaacaccgg gaactgggcg atcgcggcgc cggccttcag
     1381 gatgttgcgc ttctggtcct gtgaggcagc ggacgcaccg aacaggaaga gccgaccgag
     1441 cggtcgcagc aactggtagt tcttgcggct ggtccggccg ccgacggcgt cgaggatcac
     1501 gtcgacacca cggcccttgg tgagccggtt gacctcggcg gcgaagtcct ggttgcggta
     1561 gtcgatgggg tgatcgaggc cggcgtcgcg cagccgctcg tgcttgccgg agctggcggt
     1621 gccgtaggtg acggcgccga ggagccggca gatctggagc gccgcgaacc cgacgccacc
     1681 cgcggcggcg tggatgagga ccgactcgcc cttccgcacg ttgccgagcc ggacgagcat
     1741 caggtaggcg gtgaggtagt tgaccgggat cgccgcggcg tggacgagat cgaggccgtc
     1801 gggcaggtgc acggcctggt gcgccggaac gaccacgctc gacgactggc cgccgaagcg
     1861 ggtgagcgac acgactcggt cgccgacggc gaacccctcg accccgccgc cgacggcgtc
     1921 gaccgtgccc gacacttcgt agccaaccgt catcggcggc ttgggtgcgt cggggtacag
     1981 accgacccgg gcggagacgt cggcgaagtt cagccccgcc gccgcgacgg cgatgcggac
     2041 ctccccgcgc atcggcgacg ggtcgggggc ctcccggacc tggagcacct cgggaccgcc
     2101 gaccttcgtg atgaccacct gctgcatgcc gagagcctcc cacgtccggc acgagtacag
     2161 tcagccgatg gcacgcccgt ccgtcacacc ccggggcctg gccgtcgctc gtggtgacga
     2221 accggccgac ctgctcatcc gcggagggcg cgtgttcgtg tcctcgaccc gctcgtggat
     2281 cgagacatcc ctcgcaatcg ccgacggctg catcgtcggc tggggcgacc gggaggcact
     2341 cgatgtcgtc gacgtcgacg gagcggcact gacgccggcg ttcgtcgacg cccacatgca
     2401 tctcgagtcg accaagctct gggtcgacga gttcgtgcgg gccgtcctgc cccacggcac
     2461 caccgcagtc gcggccgacc cccacgagat cgccaacgtc ttcggcatcc ccggcgtcgc
     2521 cgcgctgatc gcagccgcgg cggatctccc gttccacttc ggcgtgtgcg cttcgtcgtg
     2581 tgtaccggcc tcgccgttcg aatcggccgg cgccgaactc tcccacaccg acgtcgccga
     2641 cctgctcgac gggcacgggg ccatcggcgt ggccgaggtc atgaacttcc ccggggtcat
     2701 cggcggcgac cccgagatgc tggcccgcat cgcgacggcc ggcagtcgtc gcgtcgacgg
     2761 ccacgcgccg ggcctccgcg gccaacagct cgacgcctac ctcgccgccg gcgtc
//
LOCUS       DAOSOA010000385         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_36238_length_2782_cov_5.169784, whole genome shotgun sequence.
ACCESSION   DAOSOA010000385 DAOSOA010000000
VERSION     DAOSOA010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36238_length_2782_cov_5.169784"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1116)
                     /locus_tag="PKY13_16130"
     CDS             complement(193..1116)
                     /locus_tag="PKY13_16130"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOT81522.1"
                     /translation="MFRVEVTRGSVVESTHEVRACAVDVSGVLVGEGSPGDADWHMFM
                     RSAAKPFQAAPAASAGVIDTLGLDDRHLAVACASHDGSAAPVQLVREILDAAGLDEFA
                     VRAGDDGQGGLIKHQCSGNHALALAYCVVMGWPTDSYLDRDHPVQQTMQAAVALAVDA
                     DPHLEADNCGMTAHRVPLRNIATAYARLGVGWAGLPGLDAVAAAMRANPYVIRQAGQA
                     DAELMAADPSLVAKHGAEGAIGIGSSAGLGVVVRVVDGAPRAWGAAGVAAARRWLVPD
                     LAGAALDRLEPAVVLDGAHRPIGELRAVWSD"
     gene            1300..>2782
                     /locus_tag="PKY13_16135"
     CDS             1300..>2782
                     /locus_tag="PKY13_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOT81523.1"
                     /translation="MSDDSGVSRSTASPYLDLLRERVVVFDGAAGTYLQQVGLTADDF
                     GSDALEGCTDLLSVTRPDVIAQMHADYFEAGADVVETNSFGAFGIPLGEYDIADRAHE
                     INMAAARIAREVANDLSTPDRPRFVAGSMGPGTKFATLGQVSYADLRDQYHEQGIGLL
                     EGGVDLFIIETQFDLLGVKAAMNGVRRAMKTVGRHVPIQTQVTIELTGRMLPGTEIAA
                     ALAALDPMRPDVIGLNCATGPSEMGEHLRYLAQHSRIPISVLPNAGLPSVVDGKMHYD
                     LTAEEFVTYQQRFVREFGVRVVGGCCGTTPEYIRQLAAGVKDIEPAHLAPVHTDGATS
                     IYSFQAFGSEDGDNAATSFLMIGERTNANGSKAFREAILAEDWDQTVQVAKDQIKDGS
                     HVLDLCVDYVGRDGTLDMDAIAQRYATQSTVPIVLDSTEPQVMEAGLQWLGGRAILNS
                     ANLEDGFHEGSRLDRVFSLAREYGAAVICLLIDEEGQARDVEWK"
BASE COUNT          441 a         1020 c          921 g          400 t
ORIGIN      
        1 gcgcacccgt tgtgatcgtc aacgggtccc cgacggagat ggacgagctc gccactgtcg
       61 tcctgagggg ttcgatcagc gaggtgctcc cgcgcatcgt ggggtgaggt ctgggaccgc
      121 cgcaccgctt ctgtcctgcg aaagcgcccc tccaggggcg gttctgcagg acagaacggg
      181 tgaagtcggg ggtcagtccg accagacggc gcggagttcg ccgatggggc gatgggcgcc
      241 gtcgagcacc accgcgggct cgagccggtc gagcgccgcc ccagcgagat cggggacgag
      301 ccaccgcctc gcggccgcca cgcccgcggc gccccacgcc cgaggggcgc cgtcgaccac
      361 gcgtacaaca acgccgagtc cggccgacga gccgataccg atcgcaccct ccgcaccgtg
      421 cttggcgacc agcgacgggt cggcggccat cagctcggcg tcggcctggc cggcctggcg
      481 gatcacgtag gggttcgccc gcatagctgc agcaactgcg tccagccccg ggagtcccgc
      541 ccatccgacg cccagacggg cataggccgt ggcgatgttg cgcagcggca cccggtgcgc
      601 ggtcattccg cagttgtccg cttcgaggtg cggatcggcg tcgacggcga gcgccacggc
      661 cgcctgcatg gtctgctgca ccgggtggtc gcggtccagg tacgagtcgg tcggccaccc
      721 catgaccacg cagtacgcca gggccagggc gtggttgccg gagcactggt gcttgatgag
      781 tccaccctgg ccgtcgtcac cggcgcgaac ggcgaactcg tcgagaccgg cggcgtcgag
      841 gatctcccgg accagctgca ccggtgccgc cgagccgtcg tgagacgcac acgccaccgc
      901 gaggtgacga tcgtcgaggc cgagcgtgtc gatgacaccc gccgaagccg ccggggccgc
      961 ctggaacggc ttcgcggccg accgcatgaa catgtgccag tcggcgtctc ccggcgaccc
     1021 ttcgcccacc agcacgccgc tgacgtcgac ggcgcaggcc cgcacctcat gtgtgctctc
     1081 gaccaccgac ccgcgggtca cctcgacacg gaacatgagc ggaccctacc ggcccggccc
     1141 ccgaatcgtg agggacggcg cgagtccgga gcgctatcat gtcgcccgca cgttgcactg
     1201 cgttgagctg acctgatggg aagggcgtga ggccccggct cgcggtgacg atccagctga
     1261 gatctcgcgg ccgctcggcc gctccgcgaa ggagttccca tgtccgatga ttcgggtgtt
     1321 tccagaagta ctgccagtcc ctacctcgac ctcctgcgcg agcgcgtggt cgtgttcgac
     1381 ggcgccgccg gcacctacct ccaacaggtc ggcctgaccg ccgacgattt cggcagcgac
     1441 gcactcgaag ggtgcaccga cctcctgtcg gtcacccgcc ccgacgtcat cgcccagatg
     1501 cacgccgact acttcgaggc cggcgccgac gtcgtcgaga cgaacagctt cggggcgttc
     1561 ggcatcccgc tcggtgagta cgacatcgcc gaccgcgccc acgagatcaa catggccgcg
     1621 gcccgcatcg cccgtgaggt cgccaacgac ctgtcgacac cggatcgccc ccgcttcgtc
     1681 gccggctcca tgggcccggg cacgaagttc gccaccctcg gccaggtgtc gtacgccgat
     1741 ctgcgcgacc agtaccatga gcagggcatc ggcctcctcg agggcggcgt cgacctgttc
     1801 atcatcgaga cccagttcga cctgctcgga gtgaaggctg cgatgaacgg cgtgcgccgg
     1861 gccatgaaga cggtcggtcg gcacgtcccg atccagacgc aggtgacgat cgagttgacc
     1921 ggccgcatgc tgccgggcac cgagatcgcg gccgccctgg ccgcgctcga cccgatgcgc
     1981 cccgatgtca tcggcctcaa ctgcgccacc ggcccctcgg agatgggcga gcacctccgc
     2041 tacctcgccc agcattcccg catcccgatc tcggtgctgc ccaacgccgg cctgccctcc
     2101 gtcgtcgacg gcaagatgca ctacgacctc accgccgaag agttcgtgac ctaccagcag
     2161 cggttcgtcc gcgagttcgg cgtccgggtc gtcggcggct gctgcggcac cacgcccgag
     2221 tacatccgcc agctcgcagc gggcgtcaag gacatcgagc cggcgcacct cgcccccgtt
     2281 cacaccgacg gcgcgacatc catctacagc ttccaggcgt tcggctccga ggacggcgac
     2341 aacgccgcca ccagcttcct gatgatcggc gagcgcacca acgccaacgg atccaaggcg
     2401 ttccgcgagg cgatcctcgc cgaggactgg gaccagacgg tccaggtcgc caaggaccag
     2461 atcaaggacg gtagccacgt cctcgacctg tgcgtcgact acgtgggccg cgacggcacg
     2521 ctcgacatgg acgcgatcgc ccagcgctac gccacgcagt cgacggtgcc gatcgtgctc
     2581 gactccacgg agccccaggt catggaggcc gggctccagt ggctcggggg ccgtgcgatc
     2641 ctcaactcgg caaacctcga ggacgggttc cacgagggct ctcgcctcga ccgggtgttc
     2701 tcgctcgccc gcgagtatgg tgccgccgtc atctgcctgc tcatcgacga ggagggtcag
     2761 gcccgtgacg tcgagtggaa ga
//
LOCUS       DAOSOA010000386         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_36519_length_2766_cov_5.857986, whole genome shotgun sequence.
ACCESSION   DAOSOA010000386 DAOSOA010000000
VERSION     DAOSOA010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36519_length_2766_cov_5.857986"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1814
                     /locus_tag="PKY13_16140"
     CDS             165..1814
                     /locus_tag="PKY13_16140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT81524.1"
                     /translation="MIRSHRLAAVALAAALVVSACGGSDDTESSSATTAPGKVDTSSD
                     ITGKGPDGSGPAPDAVTVAVRIDVDSFDPHTSLGDSAAQQAFLFLYDTMVHRTLDGEI
                     LPGVASEWEVTPTKGVFTIRDGLTCTDGTPLDATAVAASYESLGNNPGSLGKSKIFGP
                     DGMASVTADPAAKTVTIETKSPNNDMLTGLATTGFIVCPAGLADPASIKEAPAGSGPY
                     ELTDSKRGDEYTLKLRDDYAAFFDDTTIEDLPKTVTLKVITDDAAAADLVESGKIQIA
                     GILSTDAKRLLANPDLVSVPAQGYGSNAVLMMQKPSAATSDEKLRQGIAMLLDSVQGG
                     AAETQGLGTPRRTLYTPNIDCYTPDAEAYAPEYDPAAAAAFLDEAGYKVGADGKRTKP
                     DGSELTIKVVGNNTQGQIPQFIADSLEKGDIDVDLFVGTYNESIAKLLSDQFDIGSYP
                     FTDSSPLPSLWPNQIGSNAGANFGQVNNTEFDRLVNEALAVDSATDPEGRCEKWQEAE
                     KVVLEAANVVPMDQPTNNWFGNGVTFNSQFFKIDPFSIRSK"
     gene            1832..>2766
                     /locus_tag="PKY13_16145"
     CDS             1832..>2766
                     /locus_tag="PKY13_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT81525.1"
                     /translation="MARWGRFLLQRVVRVVIVSVVLITATFVTVRLVPGDPAQKAIGM
                     QTDRSPEEQAATLEQAKRDLHLDRSLPVQYAETLGAAATFDFGRSFQTNQQVTSIIGD
                     RLGSTLRLAAGGTLVMLVIGVPLGVLVGAFTNGRRRWLDVIFSGVSGFIGSIPPYVLA
                     VIGVFFFAYTWSVFPRIPEGSLLDDVLPSLALGLGPAFLLARVVRVETLDVLQQDYVR
                     TARSKWLPWRHLYGRDVLPNVLTPALTIGGVLFASLLGGAVVVEGIFGRNGLGSTLIN
                     AVQLGDYPVVSAISLLFGFMVVAVNTAVDLMLAAI"
BASE COUNT          456 a          982 c          887 g          441 t
ORIGIN      
        1 gcgaccgacg agatggccac ccagaggagg agcgcgagaa cacggcgagc gaacggaccc
       61 acgacgccga ccgtaccgcc acatgacgcg ccggtggcgc ccacccaact cgtcatcgga
      121 gatcgaagcg cgaagaggcc tcgggggagc ggtatcgttc gccgatgatc cgttcccacc
      181 gcctcgcagc cgtcgccctg gcagccgccc tcgtcgtgtc cgcctgtggt ggctccgacg
      241 acaccgaatc ctcgagcgcc acgaccgctc cgggcaaggt cgacacctcg tccgacatca
      301 cgggcaaggg ccccgacggc tccgggccgg cgcccgacgc ggtgaccgta gcggtccgca
      361 tcgacgtcga ctcgttcgat ccgcacacgt cactcggcga ctcggcggcg cagcaggcgt
      421 tcctgttcct ctacgacacg atggtgcacc gcaccctcga cggggagatc cttccgggtg
      481 tcgcgtccga gtgggaggtg acgcccacca agggggtgtt cacgatccgt gacggcctca
      541 cgtgcaccga cgggacaccg ctcgacgcaa cggcggtggc ggcgtcgtac gagtcgctcg
      601 gcaacaaccc cggcagcctc ggcaagagca agatcttcgg ccccgacggc atggcatcgg
      661 tcaccgctga cccggccgcg aagacggtca cgatcgaaac caagtcgccc aacaacgaca
      721 tgctcaccgg cctcgccacc accgggttca tcgtgtgtcc ggccggtctc gccgacccgg
      781 cgagcatcaa ggaggccccc gccgggtcgg ggccctacga gctgactgat tccaagcggg
      841 gcgacgagta cacgctcaag ctgcgtgacg actacgccgc gttcttcgac gacaccacga
      901 tcgaggacct tccgaagacg gtgacgctca aggtcatcac cgacgatgcc gctgccgccg
      961 acctcgtcga gagcggcaag atccagatcg ccggcatcct gtcgaccgac gccaagcgac
     1021 tgctcgccaa ccccgacctc gtgtcggttc ccgcccaggg ctacggctcg aacgccgtcc
     1081 tcatgatgca gaagccgtcg gcggcgacgt cggacgagaa acttcgccag ggaatcgcca
     1141 tgctgctcga cagcgtgcag ggcggcgcgg ccgagacgca gggcctcggc acaccccgcc
     1201 gtaccctcta cacgccgaac atcgattgct acacgcccga cgccgaggcc tacgcccccg
     1261 agtacgaccc ggcggctgcg gcggccttcc tcgacgaggc cggttacaag gtcggcgccg
     1321 acggtaagcg caccaagccc gacggctccg agctcaccat caaggtggtc ggcaacaaca
     1381 cccagggtca gatcccgcag ttcatcgccg actcgctcga gaagggcgac atcgacgtcg
     1441 acctcttcgt cggcacctac aacgagtcga tcgccaagct gttgagcgac cagttcgaca
     1501 tcggttccta cccgttcacc gactcgtcgc cgttgccgtc gctctggccg aaccagatcg
     1561 gctcgaacgc cggtgccaac ttcgggcagg tcaacaacac cgagttcgac cgcctggtga
     1621 acgaggcgct cgccgtcgac tcggcgaccg accccgaagg ccggtgcgag aagtggcagg
     1681 aggccgagaa ggtggtgctc gaggccgcca acgtggtgcc gatggatcag cccaccaaca
     1741 actggttcgg caacggcgtc acgttcaact cgcagttctt caagatcgac ccgttctcga
     1801 tccgctccaa atgatggtcg aggtctgacc gttggcgcgg tggggtcggt tcctcctgca
     1861 gcgggtcgtg cgcgtcgtga tcgtgtcggt ggtgctgatc accgccacgt tcgtgacggt
     1921 gcggctggtg cccggcgacc cggcgcagaa ggcgatcggt atgcagaccg accggtcacc
     1981 cgaagaacag gcggcgacgc tcgagcaggc gaagcgcgac ctgcacctcg accgctccct
     2041 gcccgtgcag tacgccgaga ccttgggcgc tgctgcgacc ttcgatttcg gccgctcgtt
     2101 ccagaccaac cagcaggtca cgtcgatcat cggtgaccga ctcggctcga cgctccgcct
     2161 ggcagcaggt ggcacgctcg tgatgctcgt gatcggcgtg cccctcggcg tcctcgtcgg
     2221 agcgttcacc aacggtcgcc ggcggtggct cgacgtgatc ttcagcggtg tcagcgggtt
     2281 catcgggtcc attccgccgt acgtgctcgc ggtgatcggc gtgttcttct tcgcctacac
     2341 ctggtcggtg ttcccccgga tccccgaggg ttccctgctc gacgacgtgc tgccgtccct
     2401 ggcgctgggg ttggggccgg cgttcctcct cgctcgtgtg gtgcgggtcg agaccctcga
     2461 cgtcctccag caggactacg tgcgcacggc acgcagcaag tggctgccgt ggcggcacct
     2521 gtacggccgc gacgtgctgc ccaacgtgtt gaccccggca ctgaccatcg gcggcgtcct
     2581 gttcgcgtcg ttgctcggcg gcgccgtggt cgtcgagggc atcttcggcc gcaacgggct
     2641 cggctcgacg ctgatcaacg ccgtgcagct cggcgactat cccgtcgtgt cggcgatctc
     2701 cctcctgttc gggttcatgg tggtcgcggt caacaccgcg gtcgatctga tgctggcggc
     2761 catcga
//
LOCUS       DAOSOA010000387         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_36524_length_2766_cov_4.584286, whole genome shotgun sequence.
ACCESSION   DAOSOA010000387 DAOSOA010000000
VERSION     DAOSOA010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36524_length_2766_cov_4.584286"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKY13_16150"
     CDS             complement(<1..366)
                     /locus_tag="PKY13_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT81526.1"
                     /translation="MSTRSTVLVLGGGSDIAAATVATMQRAGQVRVIAAARDTDAAIG
                     GLAMAAPGAELIGRRWDALDVAHHESFVNDVFDRYGPIDVVLCAVGQLGHHAGVSMAA
                     VDVDAMVRANFSGPAAALSA"
     gene            complement(366..1001)
                     /locus_tag="PKY13_16155"
     CDS             complement(366..1001)
                     /locus_tag="PKY13_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT81527.1"
                     /translation="MSRPRVVVVDDHALFRAGVIAELGDRVDVVGSAEDVSSAIEVIG
                     ATRPDVVLLDVHLPGGDGAAVMEGLRGIDEVRYLALSVSDAADDVIALVRAGARGYVT
                     KSITADELCDAVDRVAAGDAGFSPRLAGFVLDAFSAAPAPSADPELDQLTAREREVLR
                     LLARGYAYKEIARELTISVKTVESHASAVLRKLQLSNRSELSRWASERRLI"
     gene            complement(998..2221)
                     /locus_tag="PKY13_16160"
     CDS             complement(998..2221)
                     /locus_tag="PKY13_16160"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOT81528.1"
                     /translation="MPTVSPSRTPLPRESTPRTAAARSTRPPRSDDDPLVLGISADLA
                     ARLGASVPVVRAAFVVLSVAGGVGLVVYVALAALWRDRSHEPTEPTATRDAGVVLVTA
                     GAVWDLAYWWPGVRLTLVVPVGLVAMGIALGWRPGGTDASVGSSGRLRFGALAARVAG
                     GLVLCIGGLAVFVGQRVDLSTMRDTGIALAVALGGVALVLGPSVVRIARSLGAERDQR
                     IRVQERAAVAAHLHDSVLQTLTLIQKRSDDPAATAALARHQERSLRRWLYGGTDLDGA
                     ALAPDGWRAAAEQMVGEVEDLHAVAIELVMVGDGELTPAVSAALAAAREALVNAAKFS
                     GVLHLSMYCEWSGDRFSLFVRDRGKGFDLGLVDSDRRGISDSIIGRVRSVGGQATIRT
                     APGEGTEVEIWVRAR"
     gene            2356..>2766
                     /locus_tag="PKY13_16165"
     CDS             2356..>2766
                     /locus_tag="PKY13_16165"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOT81529.1"
                     /translation="MDTPVTEQPPPEPPTSEPSSSQPPPQQPPPGHGFGEWWSSLYRS
                     STERLAGGVAGGLAHRFGVPVLLVRVGFVLAAFFGIGFPLYVLAWALLPNDRGERVMG
                     HGPVRDLLTVAAVLVAGLMLFAEFDDVDYWSVVGR"
BASE COUNT          414 a          980 c          998 g          374 t
ORIGIN      
        1 ggccgacagc gcggctgccg ggccgctgaa gttggcgcgg accatggcgt cgacgtcgac
       61 ggcagccatc gacacaccgg cgtgatggcc caactgcccg actgcgcaga ggacgacgtc
      121 gatcgggccg taccggtcga acacgtcgtt gacgaacgac tcgtggtggg cgacgtcgag
      181 cgcgtcccag cggcggccga tcagctcggc gcccggcgcg gccatggcaa ggccaccgat
      241 ggcggcgtcg gtgtcgcgag cagcggcgat cacgcgcacc tgcccggcgc gctgcatcgt
      301 cgcgacggtc gcggccgcga tgtcgctgcc accccccagc acaaggaccg tggatcgggt
      361 ggacatcaga tcaacctccg ctccgacgcc caccggctca gctcgctccg gttggagagc
      421 tggagcttgc ggagcacggc cgacgcgtgg gactcgacgg tcttgacgct gatcgtcagc
      481 tcccgggcga tctccttgta cgcgtacccc cgggcaagca ggcggaggac ctcacgctcg
      541 cgggccgtga gctggtcgag ctcgggatcg gcgctcggcg ccggcgcggc gctgaacgcg
      601 tcgagcacga agccggccag ccgcggcgag aagccggcgt cgccggcggc gacgcggtcg
      661 accgcgtcgc acagctcgtc ggcggtgatc gacttggtca catagccgcg ggctccggcg
      721 cgaacgagcg cgatcacgtc gtcggcggcg tcggacacgc tgagcgccag gtatcggacc
      781 tcgtcgatgc cacggagccc ctccatgacg gcggcgccgt cgccaccggg caggtggacg
      841 tcgagcagca ccacgtcggg gcgagtggcg ccgatgacct cgatcgccga cgagacgtcc
      901 tcggccgagc cgaccacgtc gacacggtcg cccagctcgg cgatcacgcc ggcccggaac
      961 agcgcgtggt cgtcgaccac gacgacgcgg ggccggctca tcgggcccgc acccagatct
     1021 cgacctcggt gccctcaccg ggagcggtgc ggatcgtcgc ctggcctccc acggaccgga
     1081 cccgaccgat gatcgagtcg ctgatgccac ggcggtcgct gtcgacgagg ccgaggtcga
     1141 accccttgcc acggtcgcgg acgaacaggc tgaatcgatc gccggaccac tcgcagtaca
     1201 tcgacaggtg gaggacgccc gagaacttgg cggcgttcac gagcgcctcc cgcgccgcgg
     1261 ccagcgctgc ggacaccgcc ggcgtgagtt cgccgtcgcc gaccatcacc agctcgatgg
     1321 cgacggcatg gagatcctcg acctcgccga ccatctgttc ggccgccgct cgccacccgt
     1381 cgggggccag cgccgcgccg tcgaggtcgg ttccgccgta cagccatcgg cgcagcgacc
     1441 gctcctggtg acgggccagc gccgcggtcg ccgcagggtc gtcggaacgc ttctggatga
     1501 gggtcagtgt ctgcagcacc gaatcgtgga ggtgagcagc gacggcggcg cgttcctgca
     1561 cccggatgcg ctgatctcgt tcggcgccga gcgatctggc aatgcgaacg acgctcgggc
     1621 cgaggacgag cgcaactccg ccgagtgcga ccgcgagcgc gatcccggtg tcacgcatgg
     1681 tgctcagatc gacgcgttga ccgacgaaca ctgcgagacc gccgatgcag aggaccagtc
     1741 ccccggcgac gcgtgcggcg agcgccccga accgcagtct cccgctcgac cccaccgacg
     1801 cgtcggtgcc gcccggccgc caaccgaggg cgatgcccat ggccacgagg ccgacgggca
     1861 cgaccagcgt caggcgcacc ccgggccacc agtaggcgag gtcccagacc gcgccggcgg
     1921 tgacgagcac gaccccggcg tcgcgcgttg cggtgggttc ggtcggttcg tgggagcggt
     1981 cccgccacag cgctgcgagt gcgacgtaca cgacgaggcc gacgcccccg gccacactca
     2041 gcaccacgaa cgcggcccgc acgaccggca ccgacgcgcc caggcgagcg gcgagatcgg
     2101 cgctgatgcc caggacgagc ggatcgtcgt cggagcgtgg cgggcgggta gatcgggccg
     2161 cggcggttcg aggtgtcgac tctcggggca agggagtgcg ggaaggggac acggtcggca
     2221 tgtgggggcc atgatggcac cgacatccca ccgatggcat cagggcagaa ccctgagacg
     2281 atctcggggt cgtctcgggg acagatcagc gcttccccga tatcgagatc gccgccgcag
     2341 gacgacgatc gttccatgga cacccccgtg actgagcagc cgccccccga accaccgacg
     2401 tccgaaccgt cgtcgtccca gccgccgccc cagcagccgc cgcccggcca cgggttcggt
     2461 gagtggtggt catcgttgta ccggtcgagc accgaacggc tcgccggtgg tgtggccggt
     2521 ggtctcgccc accgcttcgg tgtccccgtg ttgctggtgc gggtgggctt tgtgctggcg
     2581 gcgttcttcg ggatcggatt cccgctgtac gtgctggcct gggcgttgtt gccgaacgac
     2641 cgcggcgagc gggtcatggg gcacggcccg gttcgtgacc tgttgacggt cgccgccgtg
     2701 ctcgtcgccg ggttgatgct cttcgcagag ttcgacgacg tcgactactg gtcggtcgtc
     2761 ggtcgg
//
LOCUS       DAOSOA010000388         2746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_36900_length_2746_cov_3.691564, whole genome shotgun sequence.
ACCESSION   DAOSOA010000388 DAOSOA010000000
VERSION     DAOSOA010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36900_length_2746_cov_3.691564"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKY13_16170"
     CDS             <1..1041
                     /locus_tag="PKY13_16170"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT81530.1"
                     /translation="PGFENKVFYVWFDAPIGYIAATREWAEAAPEGERDWESWWRTDR
                     GADDVWYTEFMAKDNVPFHTLGFPATLMGSGEPWRLVDQLKSFNWLNYYGGKFSTSDH
                     RGVFMTDALELLPADYWRWYLLSNAPESDDTSFTWAQFAEAVNKDLVGTLGNFVNRCA
                     TQVDRHFDGLVPAGGEVGEVEAALETRIAALIEEYRTHFDGLEFRKATRSLREIWAEG
                     NVYLEAREPWRAIKVDRDAAAVTLRTALGLARLFGHLSAPFVPETSERIAALLPEASG
                     LDGVDPSLAPSILELAPGSPFVSSGLLFQKIDDDTLAAWAGRFGGAEDQDSGGAEDPD
                     SGGADDGADPAH"
     gene            1079..2083
                     /locus_tag="PKY13_16175"
     CDS             1079..2083
                     /locus_tag="PKY13_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81531.1"
                     /translation="MATRSARAKRLRSKESPPAQGRSRSSRRARALAGNATRLLVLVA
                     IVLGSGALAGMALGYPIGSLDLGSSDAITLDQEALAGTIDAEQALVAQGDIPKSYTPA
                     DENVTAGVALIGAKYCGVTVAPEAVVGEPITRAYLDSTNHALMLSEVVKVRQQNEAGK
                     YIKELTGVFDGCSDQKYFTGDGADKVRLQISNPRKKDEPLELDYLTRTLTPVNGGTTQ
                     IVTYFQVGNVIVAIQYAGPDKPDKNLMSKAEDEILYRVAPDQFSKTAKVKGEKPMPDE
                     TTTTTAPDAVQPSPTSSPPTMATTPPTFEAPTTTRAKRTTTTKKPTTTAPAATAPPGQ
                     "
     gene            2091..>2746
                     /locus_tag="PKY13_16180"
     CDS             2091..>2746
                     /locus_tag="PKY13_16180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT81532.1"
                     /translation="MSAAYQALIADLVDEAEALDAVVANLDEAGLATPTPAEGWTITD
                     QLSHLAGFDEAGRTAIVDPDGFRSDLDRRLTEGDDPIAGYTAAGRAMTGAAVRDWWRT
                     ARVALVDAARPLDPKTRVPWYGPPMSAMSHVTARTMETWAHGQDVRDTLGEPPSVSHR
                     LRHVAHIGVGARAFSYALNGIELPETPIDVVLTAPDGSTWSWGPGDAADRVEADAFDF
                     "
BASE COUNT          457 a          971 c          940 g          378 t
ORIGIN      
        1 cccggtttcg agaacaaggt cttctacgtc tggttcgacg ctccgatcgg ctacatcgcc
       61 gcgacccggg agtgggccga ggcagccccc gagggcgaac gcgactggga gtcgtggtgg
      121 cgcaccgacc gtggcgccga cgacgtctgg tacacggagt tcatggcgaa ggacaacgtc
      181 ccgttccaca ccctcggctt ccccgccacg ctgatggggt cgggcgagcc gtggcggctc
      241 gtcgaccagc tcaagagctt caactggctg aactactacg gcgggaagtt ctcgacgagt
      301 gaccaccgcg gtgtgttcat gaccgacgcg ctcgaactgt tgccggcgga ctactggcgt
      361 tggtatctcc tgtccaacgc ccccgagagc gacgacacga gtttcacgtg ggcccagttc
      421 gccgaggcgg tcaacaagga cctcgtgggc acgctcggca acttcgtcaa ccggtgcgcg
      481 acccaggtcg accggcactt cgacggcctg gtgccggcgg gtggcgaggt gggggaggtc
      541 gaggccgccc tcgagacccg gatcgctgcg ttgatcgagg agtaccgcac ccacttcgac
      601 ggtctcgagt tccgcaaggc gacccgctcg ctccgggaga tctgggccga gggcaacgtg
      661 tacctcgagg cgcgtgaacc gtggcgggcg atcaaggtcg atcgcgatgc cgcggcggtg
      721 acgctgcgca cggcgctggg tctggcccgt ctgttcggac acctgtcggc gccgttcgtc
      781 cccgagacgt cggagcgcat cgccgcgctg ctgcccgagg cttcgggcct cgacggcgtc
      841 gatccgtcgc tcgcgccgtc gatcctcgag ctcgcaccgg gttcgccgtt cgtgtcgtcg
      901 ggcctgctct tccagaagat cgacgacgac accctcgctg cctgggcggg tcgcttcggc
      961 ggagccgaag atcaggacag cggcggagcc gaagatccgg acagcggcgg agccgacgac
     1021 ggggccgacc ccgcgcattg acgcggctgg ccgaggccgc gccactacgg tgacggccat
     1081 ggcaacgagg tcagcgcgtg cgaaacgact gcgctccaag gagtcgccac cggcacaggg
     1141 tcggtcgcgc tcgagccgcc gcgcccgtgc gctggccggc aacgccacgc gcctgctggt
     1201 gctcgtggcg atcgtgctcg gcagtggtgc gctggccggc atggcgctcg gctatcccat
     1261 tggttcgctc gacctcgggt cgtccgacgc gatcaccctc gatcaggagg cgctcgccgg
     1321 cacgatcgac gccgaacagg ccctggtggc ccagggcgac atccccaaga gctacacccc
     1381 ggcggacgag aacgtgacgg caggcgtggc actcatcggt gccaagtact gcggtgtcac
     1441 cgttgccccg gaggcggtcg tcggcgagcc gatcacccgg gcgtacctcg actcgacgaa
     1501 ccacgcgctg atgctgtcgg aggtggtcaa ggtccggcag cagaacgagg ccggcaagta
     1561 catcaaggag ctgaccggcg tgttcgacgg ctgctccgac cagaagtact tcaccggcga
     1621 cggggccgac aaggtccgcc tgcagatctc gaacccccgc aagaaggacg agccgctcga
     1681 gctcgactac ctgacccgca ccctcactcc cgtcaacggt gggacgacgc agatcgtcac
     1741 ctacttccag gtcggcaacg tcatcgtcgc catccagtac gccggccccg acaagcccga
     1801 caagaacctg atgtcgaagg cggaggacga gatcctctac cgcgtggcac ccgaccagtt
     1861 ctcgaagacg gcgaaggtca agggcgagaa gccgatgccc gacgagacca cgacgacgac
     1921 ggcccccgac gcggtgcagc cgtcgcccac gtccagcccg ccgacgatgg cgacgacgcc
     1981 cccgaccttc gaggcaccca ccacaacgcg ggcgaagcga acgacgacga cgaagaagcc
     2041 gacgaccaca gcgccggccg cgaccgcgcc gcccggacag tgagccaccg gtgagcgccg
     2101 cctaccaggc actgatcgcc gatctcgtgg acgaggccga ggccctcgac gcggtcgtcg
     2161 cgaacctcga cgaggccggc ctcgccaccc cgacgccggc ggaggggtgg acgatcaccg
     2221 atcagttgag ccacctggcc ggattcgacg aggcgggccg caccgcgatc gtcgaccccg
     2281 acgggttccg gtccgacctc gatcgccgcc tgaccgaggg cgacgacccg atcgccggct
     2341 acaccgcggc agggcgcgcc atgacgggcg ctgcggtccg ggactggtgg cgtacggcgc
     2401 gtgtcgcgct cgtcgacgct gcccgtccgc tcgatcccaa gacccgggtg ccgtggtacg
     2461 ggccacccat gagcgcgatg tcgcacgtga cggcccgaac gatggagacg tgggcgcacg
     2521 gccaggacgt gcgtgacacg ctgggcgaac cgccgtcggt cagccaccgt ctccgtcacg
     2581 tcgcccacat cggtgtcggt gcccgagcct tcagctacgc cctgaacggc atcgaactgc
     2641 ccgagacgcc catcgacgtc gtgctcaccg ctcccgacgg ttcgacgtgg tcgtggggcc
     2701 ccggagacgc ggccgaccgg gtcgaggccg acgcgttcga cttctg
//
LOCUS       DAOSOA010000389         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_37110_length_2734_cov_5.553565, whole genome shotgun sequence.
ACCESSION   DAOSOA010000389 DAOSOA010000000
VERSION     DAOSOA010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37110_length_2734_cov_5.553565"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..643)
                     /locus_tag="PKY13_16185"
     CDS             complement(5..643)
                     /locus_tag="PKY13_16185"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOT81533.1"
                     /translation="MVDVSRILALALTAFAIIAVPGPSVLFVISRGVTLGRRAGLATV
                     FGNAGGLAVQVLAVAAGLGTIVERSITVFTVIKFCGALYLVYLGVQAIRHRRSLADAF
                     AAGVEPVPLGRILREGFLVGVTNPKSIVLFTAILPQFVDPHGSAPQLQLVVLGGICLV
                     IALLSDSIWALAAGSARAWFGKRPERLATVGGVGGLVTIGLGVRLALTGRHD"
     gene            complement(674..1120)
                     /locus_tag="PKY13_16190"
     CDS             complement(674..1120)
                     /locus_tag="PKY13_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81534.1"
                     /translation="MDEMLFDQVAEVTRSMVPDDLGVLHLRARRWALKAWFGPAEPVK
                     EHYEAQLLGRQHVPGATALGLEIGFHAEHRDPADNDACLTRIQRAADWRAELGPEAEV
                     GGFIGRPDQWRRVSEIWVDPDLSDDGIAWEIAARLTDYIVAIEPHR"
     gene            1189..1569
                     /locus_tag="PKY13_16195"
     CDS             1189..1569
                     /locus_tag="PKY13_16195"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HOT81535.1"
                     /translation="MTPEATVNEFIRRVVANDLESALEMVTDDIEYDNVPIGKNIGPD
                     AMRAFLGVMAGNVDEVQFVIHRQTVTGNVVMNERTDRFRVGDAWMDLKVAGIFEVNDA
                     GQVTLWRDYFDMGEFNEQMQKLAG"
     gene            complement(1566..2243)
                     /locus_tag="PKY13_16200"
     CDS             complement(1566..2243)
                     /locus_tag="PKY13_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOT81536.1"
                     /translation="MTAITVDPPEQHPGRTPVLERWDPPSGAGSPPVGAVLTLHGGRV
                     RGRRAVGRLDLPLLRLHAIARAAHEDLAAEGVVIWSLRYAVQGWNGTTASPVADAQWA
                     LARIVATDDVPVAMLGHSMGGRTAMRVAGEANVAGVVALAPWFPPGEPLGEVAGRRLV
                     IAHGTRDHTTDPRASRRYAEDAERAGAHVRFVDVPGEGHALLRRSAWWNRFATESVLE
                     VLGRPAT"
     gene            2389..>2734
                     /locus_tag="PKY13_16205"
     CDS             2389..>2734
                     /locus_tag="PKY13_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOT81537.1"
                     /translation="MPAVTADTLTLPKLSTPAPTAVDRPVRSVTTAPSGFEGEGFPVR
                     RAFAGVNPADLDPFIHMDQMGEVEYAPYEPKGTDWHPHRGFETVTYIIDGTFAHQDSH
                     GGGGLITNGATQW"
BASE COUNT          463 a          947 c          953 g          371 t
ORIGIN      
        1 aacctcagtc gtggcgcccg gtgagggcga ggcggacgcc gaggccgatg gtgacgagac
       61 cgccgacgcc accgacggtc gccaggcgct cgggacgctt gccgaaccag gcgcgggccg
      121 agccggcagc gagcgcccag atcgagtcgc tgagcagcgc gatgacgagg cagattcctc
      181 cgagcaccac gagctgcagt tgcggcgccg agccgtgcgg gtcgacgaac tgggggagga
      241 tcgccgtgaa caacacgatc gacttcgggt tggtcacgcc gacgaggaac ccctcacgca
      301 ggatgcgacc gagcgggacg ggttcgacgc ccgcggcaaa cgcgtcggcg agcgatcgac
      361 ggtgccggat cgcctgcacc ccgaggtaca ccaggtacag cgcgccgcag aacttgatga
      421 ccgtgaacac ggtgatcgaa cgttcgacga tcgtgcccag gccggcggcg accgccagca
      481 cctgcacggc gaggccgccg gcgttgccga aaaccgtcgc caagccggcg cgacgcccga
      541 gtgtcacacc tcggctgatg acgaagagca cgctcgggcc gggcacggcg atgatcgcga
      601 acgcggtgag cgccagggcc aggatgcggg acacgtcgac catggtcggc acgctaccgc
      661 cgacccgtcg cagtcagcga tggggttcga tggcgacgat gtagtcggtg aggcgcgccg
      721 cgatctccca ggcgatgccg tcgtcggaca ggtcgggatc gacccagatc tccgacaccc
      781 ggcgccactg gtcggggcgg ccgatgaacc cgccgacctc ggcctcgggc ccgagctcgg
      841 cgcgccagtc ggcggcgcgc tggatgcgcg tgaggcaggc gtcgttgtcg gcgggatcgc
      901 ggtgctcggc gtggaacccg atctcgaggc cgagcgccgt cgccccgggc acgtgctggc
      961 ggccgagcag ctgcgcctcg tagtgctcct tgaccggttc ggccggaccg aaccacgcct
     1021 tcaacgccca ccggcgggcc cggaggtgca gcacgccgag gtcgtcgggg accatgctgc
     1081 gggtcacctc ggcgacctgg tcgaagagca tctcgtccac cccgatcttc taccaggcgc
     1141 tcctgacagc cacggcgtgc cgacggtgaa acgtggcagg ctccatcaat gactcccgaa
     1201 gccaccgtga acgagttcat ccgtcgagtc gtcgccaacg acctggaatc ggccctcgag
     1261 atggtcaccg acgacatcga gtacgacaac gtgccgatcg gtaagaacat cgggcccgac
     1321 gcaatgcgcg cgttcctggg tgtgatggcc ggcaacgtcg acgaggtgca gttcgtgatc
     1381 catcgtcaga ccgtcaccgg caacgtggtg atgaacgaac gcaccgaccg gttccgcgtc
     1441 ggcgatgcat ggatggacct caaggtcgcc ggcatcttcg aggtgaacga cgccggtcag
     1501 gtcacgctgt ggcgcgacta cttcgacatg ggcgagttca acgagcagat gcagaagctc
     1561 gcgggttagg tcgccggtcg gcccagcacc tcgagcacgc tctcggtggc gaagcggttc
     1621 caccaggccg atcgccgcag cagcgcgtgg ccctcgccgg ggacgtcgac gaaccggacg
     1681 tgggcaccgg cgcgttcggc atcctcggcg taccggcgtg acgcccgggg atcggtcgtg
     1741 tggtcgcggg tgccatgagc gatgaccagt cgccgacccg cgacctctcc caacggctcc
     1801 cccggcggga accacggagc cagtgcgacg actcccgcca cgttcgcctc gccagcgacg
     1861 cgcatcgccg tccgcccgcc catcgagtgg cccagcatgg cgacggggac gtcgtcggtc
     1921 gcaacgatcc gcgcgagcgc ccactgagcg tcggcgacgg gtgatgccgt cgtcccgttc
     1981 cacccctgca ccgcgtagcg cagcgaccag atcacgacgc cttcggcggc gaggtcctcg
     2041 tgggcggctc gggcgatggc gtgcagccgc agcaatggaa ggtcgaggcg cccgacggcg
     2101 cgccggcccc tgacccggcc gccgtgcagg gtgagcactg cgcccaccgg cggggagccg
     2161 gcgcccgacg gggggtccca tcgttcgagt accggggttc gtccggggtg ctgctctggg
     2221 gggtcgacgg tgatcgcggt cacgccgggg gttcggagga agtcggaggg tcggattgga
     2281 ccatcctgcc ccctcgtcgg ggagtccgcg ggaacaaacc ccgaaatgtt ccggttgggt
     2341 atagttgcaa tcgcaacaaa atgcgccacc gcagatcgag agggcatcat gccagccgtc
     2401 accgccgaca cactcacact cccgaagctc tccactccgg cgccgaccgc cgtggaccga
     2461 cccgttcgtt ccgtcaccac cgcaccgtcg ggcttcgagg gtgagggctt tcccgtgcgc
     2521 cgcgccttcg ccggcgtgaa tcccgccgac ctcgacccct tcatccacat ggaccagatg
     2581 ggcgaggtcg agtacgcccc ctacgaaccg aaaggcacgg actggcatcc gcaccgcggg
     2641 ttcgagaccg tcacgtacat catcgacggg acgttcgccc accaggactc ccacggcggc
     2701 ggtgggctca tcacgaacgg tgcgacccaa tgga
//
LOCUS       DAOSOA010000390         2727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_37231_length_2727_cov_6.731287, whole genome shotgun sequence.
ACCESSION   DAOSOA010000390 DAOSOA010000000
VERSION     DAOSOA010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37231_length_2727_cov_6.731287"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKY13_16210"
     CDS             complement(<1..196)
                     /locus_tag="PKY13_16210"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT81538.1"
                     /translation="MQNPRVLDLDVIDDPLAAIAALDPVRARILGQLVEPGSATTLAT
                     DLGLTRQKANYHLRVLEAQGL"
     gene            321..1541
                     /locus_tag="PKY13_16215"
     CDS             321..1541
                     /locus_tag="PKY13_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT81539.1"
                     /translation="MGLIQAALGALGGTLADQWKDFYTVPEGLSPTAALFAAVPRGQN
                     AGRGSNTKGSDGVITNGSRIVVPEGYGLVLMEQGAITGFAAEPGAYEWNSDAQDSQSI
                     FAGNGIVSPLITTSWERFKFGGRPGTQQRAYFVTLKELPNNRFGTQSEIYWDDAYMNA
                     QVGAVTRGTYTLKIVDPILFVRAWVPAKYLEPAEVFDFTDIENDAASQLFNEVVGSLA
                     PAFSLYTNDPSKGNRITKIQQDSIGFAQSLSAAVEQNYTWSGRGLQIVSTAIIAIEYD
                     ENTRELLRNVQRADALSGARGNSNLQASVAAGFEAAGENTGAPGLVGLGMAAGATGLG
                     GLQQPVPGIGAQSTPAPAPAPAAPAPAAAPAPAAAPAEEDPVAKLTKLKEMLDNELIT
                     QADYDAAKNKILGL"
     gene            1559..>2727
                     /locus_tag="PKY13_16220"
     CDS             1559..>2727
                     /locus_tag="PKY13_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81540.1"
                     /translation="MSTLPPPEEAAGQRSTEHTAAVPPPYSPPPSPVPPVQNATVDTR
                     NERLDDGINRCPRCGATDIQLRPSAGMLVCLFCRHEWAEARLDESVTTSGDLASLTGT
                     IIGSGAADIAADADAVITLKCGGCGAEVIVNTAEQMGARCHWCRHVLTVNEQIPNGAV
                     PDAVLPFTVTHDQAVAKIAEFAAKRRTFADRTFVREFTPENVVGVYMPYMVVDANVSA
                     DVAGFGEIETRRFTRGSGDDKETFYDADVYSVDRHVDFTADDLTIEASSERANMNAAV
                     NTNNIINTILPFDTENAVKWNASYLTGFTSEKRDQDVAHTTPVLEHQLLSIARAQVVA
                     SIAPYDRGVRWEQERLQVHGTRWLAMCLPVWLYSYYHEKNGEGMVHYIAVNGRTA"
BASE COUNT          474 a          950 c          888 g          415 t
ORIGIN      
        1 cgaggccctg cgcctcgagg acccggaggt ggtagttggc cttctggcgg gtgagcccga
       61 ggtcggttgc gagggtggtc gccgatccgg gctcgacgag ttgaccgagg atgcgggcgc
      121 gaaccgggtc gagcgcggcg atggccgcga gcgggtcgtc gatgacgtcg aggtcgagca
      181 cccttggatt ctgcatttga cagacttctt tgtcaattgt ccttcagcgg agcgaaatcg
      241 cgtgggcggg ccccaccatt tcgagggttc cgttcggtag gctgcagcag tcatcaacgg
      301 cgagaaagag gatctgaccc atggggctca ttcaggcagc actcggtgca ctcggcggca
      361 cgctggccga tcagtggaag gacttctaca cggttcccga gggcctctcc ccgaccgctg
      421 ccctcttcgc agcggtgccg cgtggccaga acgcgggccg tggatccaac accaagggct
      481 ccgacggtgt gatcaccaac ggcagccgca tcgtcgtccc cgaggggtac ggcctcgtgc
      541 tcatggagca gggtgcgatc accggtttcg cggccgaacc cggcgcctac gagtggaaca
      601 gtgacgccca ggactcgcag tcgatcttcg ccggcaacgg catcgtcagc ccgctgatca
      661 cgacgtcgtg ggagcggttc aagttcggtg gccgccccgg cacccagcag cgtgcgtact
      721 tcgtgacgct gaaggagttg ccgaacaacc gcttcggcac gcagtcggag atctactggg
      781 acgacgcgta catgaacgca caggtcggag cggtcacccg cggcacctac acgctcaaga
      841 tcgtcgatcc gatcctgttc gtgcgggcct gggtgccggc caagtacctc gagccggctg
      901 aggtgttcga cttcaccgac atcgagaacg acgctgccag ccagctcttc aacgaggtcg
      961 tcggctcgct cgctccggcg ttctcgctgt acacgaacga cccgtcgaag gggaaccgga
     1021 tcaccaagat ccaacaggac tcgatcggct tcgcccagag cctgtcggcc gctgtcgagc
     1081 agaactacac gtggagcggc cgcggcctgc agatcgtctc cacggcgatc atcgcgatcg
     1141 agtacgacga gaacacccgt gagctgctcc gcaacgtgca gcgcgccgat gcactctcgg
     1201 gtgctcgggg caactccaac ctgcaggcgt cggtcgccgc cgggttcgag gctgccggcg
     1261 agaacaccgg cgcccccggt ctggtcggcc tcggcatggc agccggggcg acgggtctcg
     1321 gtgggttgca gcagccggtc cccggcatcg gcgctcagag cacccctgcc ccggcgcccg
     1381 caccggcggc acccgcccct gcggcggcac ccgcccctgc ggcggcgcct gccgaggagg
     1441 atccggtggc caagctcacg aagctcaagg agatgctcga caacgagctg atcacccagg
     1501 ccgactacga cgccgccaag aacaagatcc tcgggctcta ggccctcacc accgaccgat
     1561 gtcaacgctg ccacctccgg aggaggcggc cggtcagcgc tctaccgagc acacagccgc
     1621 ggttccccct ccatactcac cgccaccatc gccggtccca ccggttcaga acgccacggt
     1681 cgacacgcgc aacgagcgcc tcgacgacgg catcaaccgg tgcccccgtt gtggcgcgac
     1741 cgatatccag ctccggccgt ccgccggcat gctcgtctgc ctgttctgcc gccacgagtg
     1801 ggccgaggct cgtctcgacg agtcggtcac gacgtccggc gacctcgcgt cgctgacggg
     1861 cacgatcatc ggttccggtg ccgccgacat cgccgccgac gccgacgctg tgatcaccct
     1921 gaagtgcggc ggttgtggcg ccgaggtgat cgtgaacacc gcggagcaga tgggcgcccg
     1981 ctgccactgg tgccggcacg tcctgaccgt caacgagcag attccgaacg gcgccgtgcc
     2041 cgacgccgtg ctccccttca cggtcaccca cgatcaggcc gtggcgaaga tcgccgagtt
     2101 cgcggcgaaa cgccggacgt tcgccgatcg caccttcgtg cgggagttca cgccggagaa
     2161 cgtcgtcggc gtctacatgc cgtacatggt cgtcgacgcc aacgtgagcg ccgacgtcgc
     2221 cggcttcggc gagatcgaga cgcgtcgctt cacccggggc tcgggcgacg acaaggagac
     2281 gttctacgac gccgacgtct actcggtcga ccgccacgtc gacttcacgg ccgacgacct
     2341 cacgatcgag gcgtcgtcgg agcgggcgaa catgaacgcc gcggtcaaca cgaacaacat
     2401 catcaacacg atcctgccgt tcgacaccga gaacgccgtg aagtggaacg ccagctacct
     2461 gacgggcttc acgtcggaga agcgcgacca ggacgtggcc cacaccacgc cggtcctcga
     2521 acaccagctc ctgtcgatcg cccgcgccca ggtcgtggcc agcatcgcgc cgtacgaccg
     2581 gggtgtgcgg tgggagcagg agcgcctgca ggtgcacggc acccgctggc tcgccatgtg
     2641 cctgccggtc tggttgtact cctactacca cgagaagaac ggcgagggca tggtccacta
     2701 catcgctgtc aacggccgca cggccga
//
LOCUS       DAOSOA010000391         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_38473_length_2659_cov_4.907450, whole genome shotgun sequence.
ACCESSION   DAOSOA010000391 DAOSOA010000000
VERSION     DAOSOA010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38473_length_2659_cov_4.907450"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /gene="aroC"
                     /locus_tag="PKY13_16225"
     CDS             <1..653
                     /gene="aroC"
                     /locus_tag="PKY13_16225"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT81541.1"
                     /translation="EVLAWVSAVHTIDATASIDPATVTLDEVEADPTRCPSPEHAAAM
                     AAAIEQARRDGDSLGGVVTCVARGVPAGLGAPVVDKLEADLAKAVMSLPASKGFEIGS
                     GFAGTLMTGREHNDAFVPGPDGRPRTVTNHSGGVQGGISNGEDIVIRVAFKPTATISS
                     PQTTVTRDNEPVELEAKGRHDPCVLPRAAPMVESVVLLVLADHWLLQRTVDVLPPR"
     gene            650..1315
                     /locus_tag="PKY13_16230"
     CDS             650..1315
                     /locus_tag="PKY13_16230"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT81542.1"
                     /translation="MTARRLILFDLDDTLSDRAGAFRNWARKWIARHGIGDPDALDWM
                     CTNDDGGYRPRDLFFADAKERFALPQRASELARDYALTAHDDYHEDPVIVGELRSLVD
                     RGARVGLVTNGAVGQQVKAEKLGIMPLLSCCVVSGLVGIRKPDPRIFALAVEEAGGPG
                     PGVPWMVGDNADYDVGGAQSAGLRGMWISHGRDWDQAQPPPDAIAVDITSAMALLRGS
                     FPD"
     gene            1335..2108
                     /locus_tag="PKY13_16235"
     CDS             1335..2108
                     /locus_tag="PKY13_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT81543.1"
                     /translation="MSYVDYDAGTPPPVPQRVYAAAVYDAYKVYRSGEDEIRALDGVT
                     VGFEAGRFTAVMGPSGSGKSTLMQCAAGLDTLTAGRTFIEDVETTHMNPTELTLIRRE
                     KLGFIFQAFNLIPALTAKENILLPLTLSRRPVDQAFFDYVVSTVGLGDRLDHTPGQLS
                     GGQQQRVAVARALVSRPAIIFGDEPTGNLDSRSSAEVLGFMRSAVDQWHQTVVIVTHD
                     PVAASHTDAVLFMADGKVVDHLERPTAEAVFDRMKTMGR"
     gene            2110..>2659
                     /locus_tag="PKY13_16240"
     CDS             2110..>2659
                     /locus_tag="PKY13_16240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT81544.1"
                     /translation="MWSIATKTLRANLSRFVATLVAIAVGVGFLTAGTMITNSIENSL
                     GGEIDRQYEGVDAAVGPRSEDSQPGANTFAVSVLDTVKGADGVADAAGIVIGSTKLPA
                     SLTDEGDDDDTDSAGDVFAQGPTVRAWIDNDRLNPIDLVDGTAPGPGEVTVDQGLADK
                     YDIELGQQLELSTGSGAKSYRVV"
BASE COUNT          409 a          959 c          887 g          404 t
ORIGIN      
        1 tcgaggtgct ggcgtgggtc tccgccgtcc acacgatcga cgcaacggcc tcgatcgacc
       61 cggcgacggt cacgctcgac gaggtcgagg ccgacccgac ccgctgtccc tcacccgagc
      121 acgccgcagc catggccgcc gcgatcgagc aggcccgccg cgacggcgac tcgctgggcg
      181 gcgtcgtcac ctgtgtcgcc cggggtgtcc ctgccggtct cggcgcgccg gtcgtcgaca
      241 agctcgaagc ggatctggcc aaggccgtga tgtcgttgcc ggcgtcgaag ggattcgaga
      301 tcggctcggg tttcgctggc acgctgatga ccggccggga gcacaacgac gcgttcgtcc
      361 ccgggcccga cggccgtccc cgcacggtca ccaaccattc cggcggtgtg caggggggca
      421 tctccaacgg cgaggacatc gtgatccggg tcgcgttcaa gccgacggcc acgatctcgt
      481 cgccgcagac gacggtgact cgcgacaacg agccggtcga gctcgaggcc aagggtcgcc
      541 acgacccgtg tgtgctcccc cgggcggcgc cgatggtgga gtcggtggtg ctgctcgtcc
      601 tcgccgatca ctggctgttg cagcgcaccg tcgacgtgct gccgccccgg tgaccgctcg
      661 ccggctcatc ctcttcgacc tcgacgacac cctctcggac cgggccggag cgtttcgcaa
      721 ctgggcgcgg aagtggatcg cccgccacgg catcggcgac cccgacgcgc tcgactggat
      781 gtgcacgaac gacgacggcg gctatcggcc ccgcgacctg ttcttcgccg acgccaagga
      841 gcggttcgcc ttgccccagc gggcgtctga gttggcgcgg gactacgcgc tgacggcgca
      901 cgacgactac cacgaagacc cggtcatcgt cggcgaactc cgttcgctcg tcgatcgtgg
      961 cgcccgggtc ggtctcgtca ccaacggcgc ggtcggccaa caggtcaagg ccgagaagct
     1021 cggcatcatg ccgctgctgt cgtgctgtgt ggtctcgggc ctggtcggga tccgcaagcc
     1081 cgacccgcga atcttcgcgc tcgctgtcga ggaggcaggt ggccccggcc ccggcgtgcc
     1141 ctggatggtc ggcgacaacg ccgactacga cgtcggcggc gcccagtcgg ccggcctgcg
     1201 cggcatgtgg atctcccatg gtcgagactg ggaccaggcc cagccgcccc ctgacgccat
     1261 cgcggtcgac ataacatccg ccatggccct cctacggggc tcgttccccg attgatgccc
     1321 ttggaggcac ccccgtgagc tacgtcgact acgacgccgg cactcccccg cccgtccccc
     1381 agcgtgtcta cgctgccgcc gtgtacgacg cctacaaggt gtaccggagt ggcgaggacg
     1441 agatacgagc gctcgacggc gtcaccgtcg gcttcgaggc cggccgcttc accgcggtta
     1501 tgggcccgtc gggctcgggc aagtcgaccc tcatgcagtg cgccgccggc ctcgacaccc
     1561 tcacggccgg acggaccttc atcgaggacg tcgagacgac gcacatgaac cccaccgagc
     1621 tcaccctgat tcggcgcgag aagctgggct tcatcttcca ggcgttcaac ctgatcccgg
     1681 cgctcaccgc gaaggagaac atcctccttc ccctgacgct gtcgcgtcgc cccgtcgacc
     1741 aggcgttctt cgactacgtc gtgtcgaccg tcggcctcgg cgaccgcctc gaccacacgc
     1801 cgggccagtt gtcggggggc cagcagcagc gcgtcgccgt cgcccgggcg ctcgtcagcc
     1861 ggccagcgat catcttcggc gacgaaccca ccggcaacct cgactcacgg tcgagcgccg
     1921 aggtgctcgg gttcatgcga tcggcggtcg atcagtggca ccagacggtg gtgatcgtca
     1981 cccacgatcc ggttgctgcg tcgcacaccg acgccgtgct gttcatggcg gacggcaagg
     2041 tcgtcgacca cctcgaacgt ccgacggccg aggccgtctt cgaccgcatg aagacgatgg
     2101 ggcgctgaca tgtggagcat cgcaaccaag accctgcggg ccaacctctc gcgtttcgta
     2161 gccacactcg tggccatcgc cgtcggtgtc gggttcctca ccgccggcac gatgatcacc
     2221 aactcgatcg agaactcgct gggcggggag atcgatcgcc agtacgaagg cgtcgacgcc
     2281 gcggtcgggc cccggtcgga ggactcccag ccgggcgcca acaccttcgc cgtcagcgtg
     2341 ctggacacgg tgaagggcgc cgacggcgtg gccgatgcgg cgggcatcgt gatcggttcc
     2401 acgaagctgc cggcgtcgct gaccgacgag ggcgatgacg acgacaccga ctccgccggc
     2461 gacgtgttcg cccagggccc cacagtgcgg gcgtggatcg acaacgaccg tctgaacccg
     2521 atcgacctgg tcgacggcac cgcgccgggg cccggcgagg tcactgtcga ccaggggctg
     2581 gccgacaagt acgacatcga gcttggacag caactcgaac tgagcaccgg ttcgggtgcc
     2641 aagagctacc gggtcgtcg
//
LOCUS       DAOSOA010000392         2634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_38931_length_2634_cov_5.653354, whole genome shotgun sequence.
ACCESSION   DAOSOA010000392 DAOSOA010000000
VERSION     DAOSOA010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38931_length_2634_cov_5.653354"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..957
                     /locus_tag="PKY13_16245"
     CDS             28..957
                     /locus_tag="PKY13_16245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT81545.1"
                     /translation="MTQTLDPPALGVALRGLVKTFPSPAGPVQAVRGIDLDIACGETV
                     ALLGPNGAGKSSTIEMILGLSKPDAGTVDVLGLSPKQAIQRGAIGAMLQVGAVLGELS
                     VRELVDMMGSLFPTPLGVDETLELAGIGDIAERRTNRLSGGQTQRVRFAVAMVSDPDL
                     IILDEPTVAMDVESRVAFWATMRSFTERGKTVIFATHYLEEADDYADRIVLMARGEIV
                     ADGTAAELRAASGGRHIKAVLAGASSAALESLAGVVSATIHGEAIDLACADSDVAIRA
                     LLAAYPDVHEIEIVGAGLEHAFLELTGEAGGDR"
     gene            954..1754
                     /locus_tag="PKY13_16250"
     CDS             954..1754
                     /locus_tag="PKY13_16250"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT81546.1"
                     /translation="MTAQPTAPSTAPPTRPTRRGLNATYLRYELLRLFRNKRSFIFSL
                     VFPLALFLVIGGSNKDQIMDLGSYRVKFVLYYMVSMAGYGAMIAAISGGARIATERQT
                     GWNRQLRLTPLPVTTYFATKVITSYVMSAASIVLLYAAGLTFGVRIESAGRWISMTGL
                     LLIGVLPFVALGIAIGHLLTTDAMGPAVGGGSAFFGFLGGQWFPLPASGVLRQIGELV
                     PSFWITQASHIGIGGAAWSVKGWLVIAVWAAAMSGWAAWAYRRDTKRL"
     gene            complement(1763..>2634)
                     /locus_tag="PKY13_16255"
     CDS             complement(1763..>2634)
                     /locus_tag="PKY13_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81547.1"
                     /translation="AASASASASKRVAAPTTTPPCRRGLSAPASDVVTAAVAALDDDL
                     CVSGAVESFVEADVVPLTDRDGKPQGVVIAGPGLPAVQLTTAEWASYREIAGRAQPDN
                     SVTFGGYPTAVEHGDDPQCVTIELTTGGLIAGRRSDTQMFWIPQQARSLWESEGGLEG
                     RLGFPTSNVFLSGEELRLEFEHGYLTARLDESNPGAVSWLPFDVASVGAQYVDDPGAE
                     LRPIGDLTGRVLRQAGGTTWFIDDDDIRHWIADGDTWNCLGAEQRKVGGDIAGYAVAT
                     LELGPPATCDLVR"
BASE COUNT          373 a          888 c          935 g          438 t
ORIGIN      
        1 tgtcggccga gtggttgact tcgagcgatg acgcagaccc tcgatcctcc cgccctcggt
       61 gtcgcgctcc ggggactcgt gaagacgttt cccagcccag cggggccggt gcaagcggtc
      121 cggggtatcg acctcgacat cgcttgcggt gagaccgtcg cgctgctcgg cccgaacggg
      181 gccggaaagt cgtcgaccat cgagatgatc ctcggactct cgaagcccga cgccgggacg
      241 gtcgacgtgc tgggcctgtc gccgaagcag gcgatccagc ggggagcgat cggcgcgatg
      301 ttgcaggtcg gcgcagtcct gggtgagctg tcggtgcgcg agctcgtcga catgatgggc
      361 tcgctgttcc cgacgccgct cggtgtcgac gagaccctcg agctggccgg catcggcgac
      421 atcgcggagc ggcgcaccaa ccgcctgtcg ggtgggcaga cgcagcgggt ccgtttcgcc
      481 gtggccatgg tgtcggatcc cgacctgatc atcctcgacg aaccgaccgt ggccatggac
      541 gtggagtcac gggtcgcctt ctgggcgacg atgcgttcgt tcaccgagcg gggcaagacc
      601 gtcatcttcg cgacgcacta cctcgaggag gccgacgact acgccgaccg catcgtgctg
      661 atggcccgcg gtgagatcgt cgcggatggc accgccgccg agctgcgagc agcatccggt
      721 ggtcgccaca tcaaggcggt cctcgccggg gcctcctcgg cagcgctgga atctctcgcc
      781 ggggtcgtct cggcgaccat ccacggcgag gccatcgacc tcgcctgcgc cgactccgac
      841 gtcgcgatcc gggcattgct cgcggcgtac cccgacgtgc acgagatcga gatcgtcggt
      901 gcgggactgg agcacgcgtt cctcgagttg accggcgagg ccgggggcga ccgatgacgg
      961 cgcagcccac ggcaccgtcc acggcgccgc ccacgaggcc gacgcggcgg ggcctcaacg
     1021 ccacgtatct gcgctacgag ctgctccgcc tgttccgcaa caagcgatcg ttcatcttct
     1081 cgctggtgtt cccgctggcg ctctttctcg tcatcggcgg gtcgaacaag gatcagatca
     1141 tggatctcgg ctcctaccgg gtgaagttcg tcctctacta catggtgtcg atggccgggt
     1201 acggcgcgat gatcgccgcg atctccggtg gggcccgaat cgcaaccgaa cggcagaccg
     1261 gctggaaccg ccagctgcgc ctcacgcccc tgccggtcac cacctacttc gccacgaagg
     1321 tgatcacgtc ctacgtgatg tcagctgcca gcatcgtcct gctctatgcc gccggcctga
     1381 ccttcggagt gcggatcgaa tccgccggcc gatggatctc gatgacggga ctgctcctga
     1441 tcggtgtgct gcccttcgtc gctctgggca tcgcgatcgg tcacctcctg acgaccgacg
     1501 cgatggggcc ggccgtgggt ggcggctcgg cgttcttcgg gttcctcggt ggccaatggt
     1561 tcccactgcc cgccagcggc gtcctgcgcc agatcggtga actcgtcccg tcgttctgga
     1621 tcacgcaggc gagccacatc ggcatcggcg gcgccgcctg gtcggtcaag ggctggctcg
     1681 tgatcgccgt ctgggcagct gccatgagcg gttgggcggc gtgggcctac cgccgggaca
     1741 cgaagcggct ctgagctcgg cgctaccgga cgaggtcgca ggtcgcgggc ggaccgagtt
     1801 cgagggtcgc gaccgcgtag ccggcgatgt cgccgccgac cttgcgctgc tcggcgccca
     1861 ggcaattcca cgtgtcgccg tcggcgatcc agtgtcggat gtcgtcgtcg tcgatgaacc
     1921 aggtggtgcc accggcttgg cggagcacac ggccggtaag gtcaccgatc gggcggagct
     1981 cagcgccggg gtcgtcgacg tactgggcgc cgacggacgc gacgtcgaac gggagccagc
     2041 tcacagcgcc cgggttcgac tcgtcgaggc gggcggtgag gtagccatgc tcgaactcga
     2101 gccggagttc ctcgccggac aggaagacgt tggacgtggg gaacccgagg cgcccttcga
     2161 gccctccctc gctctcccac aggctccgtg cctgttgggg aatccagaac atctgcgtgt
     2221 cggagcggcg tccggcgatg agcccgccgg tcgtcagctc gatggtgacg cactggggat
     2281 cgtccccgtg ctcgaccgcg gtggggtatc cgccgaacgt gaccgagttg tcgggttgtg
     2341 cccggccggc gatctcgcgg tacgaggccc actcggcggt ggtgagctgc acggccggta
     2401 ggccggggcc cgcgatcacg accccctggg gtttgccgtc gcggtcggtg agcgggacga
     2461 cgtccgcttc gacgaaggac tcgacggcgc cggagacgca gaggtcgtcg tcgagggcgg
     2521 cgacggcggc ggtcacgaca tccgatgcag gtgcgctcaa cccgcgtcgg cacggcggcg
     2581 ttgttgtcgg ggccgccacc cgcttcgacg ccgacgccga cgccgacgcg gctg
//
LOCUS       DAOSOA010000393         2630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_39019_length_2630_cov_4.429126, whole genome shotgun sequence.
ACCESSION   DAOSOA010000393 DAOSOA010000000
VERSION     DAOSOA010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39019_length_2630_cov_4.429126"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PKY13_16260"
     CDS             <1..890
                     /locus_tag="PKY13_16260"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT81548.1"
                     /translation="MVPPPVVLALDVGGTKLAAALVGADGSIDRRATVPTPATGTAGE
                     LWDAVCGLVDPLLDASPAVVGVGCGGPMERGGSTVSPLNIPAWREFPLLERLGDRCGV
                     PVAIDNDAKALALGEGRWGAAVGVDDYLAMVVSTGVGGGIVLDGRLLDGAAGNAGHIG
                     HVIVEPDGRRCACGGRGCLEAETSGPAIEAMTGRPPAEASVEVRRRTGRLVGRAVASV
                     AALLDLRLALVAGSVALGFGAEFFDAAQAELDESARISFARGVVIGPAGLGGDGPILG
                     AAAVGLASLPSGITPPEAQ"
     gene            901..2124
                     /locus_tag="PKY13_16265"
     CDS             901..2124
                     /locus_tag="PKY13_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT81549.1"
                     /translation="MTPEYPAETAEFRKGIQDFLAANLPEGWTGLGGIPTDDVLPFLE
                     QWRKVLHESRLLAPQWPAEYGGGGLSNLETVIQFEEFAKVGAPTGGPNDVFNIGMIGN
                     TILHWGTPEQKAHYIPKLLSNEHIWCQGYSEPNAGSDLAGVGCKVVLDGDEYVINGQK
                     IWTTYGHLANHIFVVGRTDTTGARPHDGITFLLVDMDQPGVEVRPIKMMSGDSEFNEV
                     FFTDVRCPKENILGEPNKGWAVAMTLLGFERGEAGAVLPIRFREELDRLVAMAKETGR
                     NTDPLIRQRIAWCYSKVEIMRYLGMRTLTLFLAGKEPGPDAAITKLFWSEYHKVVNQL
                     AVDILGPAAMVPSGRWPSNSISCDDPGSPNDSASWVGTMYTSMGGTIYAGTSQIQRNI
                     IGERILGLPKEPSGR"
     gene            2165..2422
                     /locus_tag="PKY13_16270"
     CDS             2165..2422
                     /locus_tag="PKY13_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81550.1"
                     /translation="MTALLGAVVVVLRHPDLWGTALRQTRLLARPGWWRRPPFLPVPD
                     PEYLRFRLLTAYGGDGTAAPDPSDLIAYLRWCRAYPQVIAH"
BASE COUNT          366 a          953 c          892 g          419 t
ORIGIN      
        1 cgatggtccc ccctccggtt gtgctcgccc tcgacgtcgg cggcacgaag ctcgctgccg
       61 cgctggtagg cgccgacggc agcatcgatc gacgtgcgac cgtgcccacc ccggcgacgg
      121 ggaccgccgg tgaactctgg gatgcggtat gtggcctcgt cgatccgctg ctcgacgcgt
      181 cacccgcggt cgtcggcgtc gggtgcggcg gcccgatgga acgcggcggt tcgacggtat
      241 cgccgctcaa catcccggcg tggcgggagt tccccctcct cgaacgcctc ggcgaccggt
      301 gcggtgtgcc ggtcgcgatc gacaacgacg ccaaggcgct cgcactcggc gagggccggt
      361 ggggcgccgc ggtgggcgtg gacgactacc tcgccatggt cgtgtcgacc ggcgtcggcg
      421 gtggcatcgt gctcgacggt cggctcctcg acggtgctgc gggcaacgcg ggccacatcg
      481 gccacgtgat cgtcgagccc gacggccgtc ggtgcgcgtg cggtggtcgc gggtgtctcg
      541 aggccgagac ctcgggcccg gcgatcgaag ccatgacggg gcggcccccc gccgaggcat
      601 ccgtcgaggt ccgccgacgc accggccgcc tcgtcgggcg ggccgtcgcc tcggtggcgg
      661 cgctcctcga tctgcgcctg gcgctcgtcg ccggttcggt ggcgctcggg ttcggcgccg
      721 agttcttcga cgcggcccag gccgagctcg acgagtccgc ccgcatctcg ttcgcccgcg
      781 gtgtggtcat cggtcccgca ggtctcggcg gtgacgggcc gatcctcggc gcggccgcgg
      841 tcggattggc gtcgcttcca tctggaatca ccccaccgga ggcgcagtag tcttggcggc
      901 gtgacccccg agtatcccgc tgagaccgcc gagttccgca agggaatcca ggacttcctg
      961 gccgccaacc tgcccgaggg ctggaccggc ctgggtggca tccccaccga cgacgtgctg
     1021 ccgttcctcg agcagtggcg caaggtgctg cacgagtccc gtctcctcgc accgcagtgg
     1081 cccgccgagt acggcggtgg cggtctgtcc aacctcgaga ccgtgatcca gttcgaggag
     1141 ttcgccaagg tgggcgcccc caccggcggc cccaacgacg tgttcaacat cggcatgatc
     1201 ggcaacacga tcctgcactg gggcaccccc gagcagaagg cgcactacat ccccaagctg
     1261 ctcagcaacg agcacatctg gtgccagggc tacagcgagc cgaacgccgg ctccgatctc
     1321 gccggggtcg gctgcaaggt cgtgctcgac ggcgacgagt acgtcatcaa cggccagaag
     1381 atctggacga cctacggcca cctcgccaac cacatcttcg tggtcggtcg caccgacacc
     1441 accggtgccc gcccccacga cggcatcacg ttcctgctgg tcgacatgga ccagcccggc
     1501 gtcgaggtcc gtcccatcaa gatgatgtcg ggcgacagcg agttcaacga ggtgttcttc
     1561 accgacgtcc gttgccccaa ggagaacatc ctgggcgagc ccaacaaggg ctgggccgtc
     1621 gcgatgaccc tgctcgggtt cgagcggggc gaggccggcg ccgtgctgcc gatccgcttc
     1681 cgcgaggagc tcgaccgcct cgtggcgatg gccaaggaga ccggtcgcaa caccgacccg
     1741 ctcattcgcc agcgcatcgc ctggtgctac agcaaggtcg agatcatgcg gtacctcggt
     1801 atgcggaccc tcacgctgtt cctggcgggc aaggagccgg ggcccgatgc ggccatcacc
     1861 aagctcttct ggtccgagta ccacaaggtc gtgaaccagc tcgccgtcga catcttgggc
     1921 ccggccgcca tggtgccgtc gggtcgttgg ccgtccaact cgatctcgtg cgacgacccg
     1981 ggctcgccca acgacagcgc ctcgtgggtc ggcaccatgt acacctcgat gggcggcacg
     2041 atctacgccg gcacgtcgca gatccagcgc aacatcatcg gcgagcgcat cctggggctg
     2101 cccaaggaac cgtcgggacg gtgatggcgc cggcagggtc gatggccgct cggtcggggc
     2161 gtcgatgacg gcgctcctgg gcgcggtggt cgtcgtgctg cggcaccccg acctgtgggg
     2221 cacggccctt cgtcagaccc gattgttggc ccgaccgggg tggtggcgac gcccaccgtt
     2281 cctgccggtt cccgatccgg agtacctccg gttccgcctc ctcaccgcct acggcggcga
     2341 cggcaccgcg gcccccgatc cgtccgatct gatcgcgtac ctccgctggt gccgggccta
     2401 cccccaggtc atcgctcact agtccggtcg gtcgccgtgc cgtccgaggt ctgaaccgca
     2461 ccgcacgccg agcgtgcgcc cgggttcaca cctccccgtc ggacgggccc tgggtcccgg
     2521 tgtcagtggc atcgggcagg atgctcacca tgccggtcaa cgccttgcgc gttgtgtccg
     2581 agctcgcagc ctctcaacac aacgtcgtca ctcgttccca ggctgctgcc
//
LOCUS       DAOSOA010000394         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_39080_length_2626_cov_5.369117, whole genome shotgun sequence.
ACCESSION   DAOSOA010000394 DAOSOA010000000
VERSION     DAOSOA010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39080_length_2626_cov_5.369117"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKY13_16275"
     CDS             <1..223
                     /locus_tag="PKY13_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81551.1"
                     /translation="ADIDLAGARVIDSHVHRWRFARAAELLPEAFLLLGGVPPAVAAG
                     DGFGTSGRVEGAATSGHLAATAITGLLRG"
     gene            297..986
                     /locus_tag="PKY13_16280"
     CDS             297..986
                     /locus_tag="PKY13_16280"
                     /inference="COORDINATES: protein motif:HMM:NF021371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HOT81552.1"
                     /translation="MLQGWYDLASVHWPYDPAEIQRLLPAGLRVDTCGGTAWVGLIPF
                     HMRRIRIPGCPPFGRWSTFPETNVRTYVVAPDGRRAVWFFSLDISRLVPATVARLTYG
                     LPYCWGTMSISHRGPDVIEYTASRRRPHRPATSRLAIRIGDPVDDEDLTEVERFVTAR
                     WALASRFAGLNAWAQVDHPPWPLRRATLVHCGDSLVRGAGLTRPTGEPTVLWSPGVEV
                     RIGRPHRLRLR"
     gene            1090..>2626
                     /locus_tag="PKY13_16285"
     CDS             1090..>2626
                     /locus_tag="PKY13_16285"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT81553.1"
                     /translation="MSNTTSRHLRRRAHSAALVLLVVATSGCQLPSWLVGAVAEAEPE
                     VPVTTPAPPRQPVPTSAITPPTTTPPTPPTPPPTTPPTPPLTTAPTPASALTLCSTGT
                     ASGPSDPPSGAVTIPVGSDPSAITGAHGAGTTYWFARGTHTPSAAIDAQAGDRFLGAP
                     GAVLDGGFAGIAAFTGGGAGVTIRGLTIQRFGTTDGRGWVNGAAINRSVAARWTVEDN
                     TIRLNDGNALFLGDGSTTRRNCFSENGQTGLAAPAQQRSGGYISLTGIVVTGNEIRGN
                     NRNDIESAPGACTGCTGGMKLWQTTGAIVSGNTVVDNHGVGIWLDNNNVDALVDDNIV
                     AGNDREGIMVETSYNSRVTNNVVTGNAVVDGRRRSGSFPIAGVFISNSGGSPRLAGTV
                     GIEVSGNRIEDNWNGVVVFWDADRYCGSASDTSVGHCTLGGATLASCATQVEQPPSPD
                     AFVENCHWGSVGVSVHDNEFVTSAALVAGCNGRCGRNGLIASAAPASTKVRLADGTVR
                     TVRN"
BASE COUNT          406 a         1006 c          847 g          367 t
ORIGIN      
        1 cgccgacatc gacctcgccg gcgcacgcgt gatcgactcc cacgtccacc gctggcggtt
       61 cgcccgagca gcggagctgc ttcccgaggc attcctcctg ctgggcggcg tgcctcccgc
      121 ggtcgccgcc ggcgacgggt tcggcacgag cggccgcgtc gaaggggcgg cgacgtcggg
      181 ccacctggcg gcgacggcga tcaccggcct cctccgggga tgacggtgcc ctcccgatga
      241 accacggcgt cgagccaatc accactgact gccctcggcc cgttcgacgg cccgtgatgc
      301 tgcagggctg gtacgacctc gcgtcggtgc actggccgta cgaccccgca gagatccagc
      361 gcctgctccc cgccggcctc cgcgtcgaca cctgcggagg aacggcatgg gtcgggctca
      421 tcccgttcca catgcgtcgc atccgcatcc ccggatgccc accgttcggt cgatggtcga
      481 cgtttcccga gaccaacgtg cgcacctacg tcgtcgctcc cgatggccgc agagccgtgt
      541 ggttcttcag cctcgacatc tcgaggctcg tgccggccac cgtcgcccgg ctcacctacg
      601 ggctccccta ctgctggggg accatgtcga tctcacaccg tggtcccgac gtcatcgaat
      661 acacggcgtc gaggcgtcga ccgcaccggc cggcgacgag tcgactggcg atccggatcg
      721 gcgatccggt ggacgacgag gatctcaccg aggtcgaacg cttcgtcacg gcgcgctggg
      781 cactcgcctc gcggtttgcc ggactgaacg cctgggcgca ggtcgaccat cctccctggc
      841 cgctccgacg ggcgacgctc gttcactgtg gcgactcgct ggtgcgaggc gccgggctga
      901 cccgcccgac gggcgagccg accgtcctgt ggtcgccggg cgtcgaggtg cggatcggac
      961 gaccacaccg gctccggctg cgctgacctc gggctagcct cagtcctggg tcgcccggcc
     1021 catgactcag gtcgaatttc acatcttctg ggcttcactc gtccgcctcg agcgccgata
     1081 gcaacgaaga tgagcaacac cacctcgcgc cacctgcgtc ggcgcgccca ttccgcggcc
     1141 ctcgtgctgc tggtcgtcgc cacgagcggc tgccagctcc cgagctggct ggtcggggcc
     1201 gtcgccgagg ccgaacccga ggtccccgtg accacgccgg cgccgccacg ccaaccggtg
     1261 ccgacctcgg ccatcactcc cccgacgacc acgccaccca cacctccgac accgcctccg
     1321 accacgccgc cgacaccgcc actgaccacg gcgccaaccc ccgccagcgc actcacgctg
     1381 tgctcgacgg gcacggcgtc gggcccgtcc gaccctccgt ccggcgcggt cacgatcccc
     1441 gtcggcagcg acccctcggc gatcaccggc gcccacgggg ccggcacgac ctactggttc
     1501 gcccgcggga ctcacacgcc gtcggcggcg atcgacgccc aggccggcga ccggttcctc
     1561 ggcgcccccg gcgcggtgct cgacggcgga ttcgccggca tcgccgcgtt caccggtggc
     1621 ggcgcgggcg tgacgatccg cggcctgacc atccagcgat tcgggacgac cgacggccgc
     1681 ggctgggtca acggcgcagc gatcaaccgc tccgtcgccg cccggtggac cgtcgaggac
     1741 aacacgatcc gcctcaacga cggcaacgcg ctgttcctgg gcgacggatc caccactcgc
     1801 cgcaactgct tctcggagaa cggacaaacc ggtcttgccg ctccagcgca gcagcgcagc
     1861 ggcggctaca tctcgctcac cggcatcgtc gtcaccggca acgagatacg tggcaacaac
     1921 cgcaacgaca tcgaatcggc accgggagcg tgcaccggct gcaccggagg catgaagctc
     1981 tggcagacga cgggggcgat cgtctccggc aacaccgtcg tcgacaacca cggcgtcggc
     2041 atctggctcg acaacaacaa cgtcgatgcg ctcgtcgacg acaacatcgt cgccggcaac
     2101 gaccgcgaag ggatcatggt cgagacgagc tacaactcgc gggtcacgaa caacgtcgtg
     2161 accggcaacg ccgtcgtcga cggccgccga cggtcggggt ccttcccgat cgccggagtg
     2221 ttcatctcga attccggagg ctcgccacga ctcgccggca ccgtcggcat cgaggtgagc
     2281 gggaaccgga tcgaggacaa ctggaacggg gtcgtcgtgt tctgggacgc cgatcgctac
     2341 tgcggcagcg cgtcggacac ctcggtcggc cactgcaccc tcggcggggc gacgctcgcg
     2401 tcgtgcgcga cgcaggtgga acaaccaccg tcacccgacg ccttcgtcga gaactgccac
     2461 tggggctcgg tcggggtcag cgtccacgac aacgagttcg tcacgtccgc agcgctcgtc
     2521 gccggatgca acggtcggtg cgggcgcaac gggctgatcg cctcggcagc accggcgtcg
     2581 acaaaggtcc gactcgccga cgggacagtc cgcaccgtcc gcaacc
//
LOCUS       DAOSOA010000395         2596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_39661_length_2596_cov_6.033451, whole genome shotgun sequence.
ACCESSION   DAOSOA010000395 DAOSOA010000000
VERSION     DAOSOA010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39661_length_2596_cov_6.033451"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PKY13_16290"
     CDS             complement(<1..960)
                     /locus_tag="PKY13_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT81554.1"
                     /translation="MFPHRALPVLLALALVGAGCSSKSNDVAVGDDANPTTAAPKQYQ
                     PIWADTPCTLVEVPDGSTVTCGTLTVPENRADPDGNQVVLPVVRIHSTAASPQPDPVV
                     YLHGGPGIGTLGNGIAGRVRNPVLAERDLIVFDQRGTGLATPSLECPEREEAFLAALS
                     DPAPFADELESFDTSLSRCYERLTDEGYDLDQYNSQTNAADLADLRVAMGIDEWNLWG
                     VSYGTRLALTAMRSYPEGIRSVVLDSVYPPSAGSADATVKSGERAFDALSNGCATDPV
                     CHMKYPDVKADLEKLAADLDAAPFAFDYTPEGGTPITLHLTGND"
     gene            complement(1034..1972)
                     /locus_tag="PKY13_16295"
     CDS             complement(1034..1972)
                     /locus_tag="PKY13_16295"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT81555.1"
                     /translation="MNVIVTGAAGFIGSTLVDRLLAEGHDVVGIDCFTDYYDVALKRS
                     NIADALTNPRFRLVEENLLEADLDALFAGVDVVWHLAGQPGVRLSWSDGFDQYASRNI
                     NATQRVLESARNAGVGRVVYASSSSVYGNAERYPTLETDRPEPYSPYGVTKLAGEHMC
                     ILYSANWGLPTVSLRYFTVYGPRQRPDMAHHRLINAAIDQTPFPMFGDGSQERSFTYV
                     GDVVNATYLAGVTPDVAPGTVLNVAGRFSCSLAELIDMVGDAVGQPVPLDRRPPQPGD
                     AQKTGGDTTLIEKVLGWTAEVSLEEGIAAQALHQKH"
     gene            complement(2128..>2596)
                     /locus_tag="PKY13_16300"
     CDS             complement(2128..>2596)
                     /locus_tag="PKY13_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOT81556.1"
                     /translation="IADFFRPYDPETGTGDLDYAAFHIVTMGERISEYCAGLFADDRY
                     QEYLMMHGIGVEMAEALAEYWHHRIRTEWGFVDEDGPSIAGLFRQQYRGGRYSSGYPA
                     CPNLEDNATIDRLLDGGRIGIEVNEETGWQYQPEQTTSALICHHPRAKYFVAR"
BASE COUNT          389 a          779 c          992 g          436 t
ORIGIN      
        1 gtcgttgccg gtgaggtgca acgtgatggg ggtcccaccc tcgggcgtgt agtcgaacgc
       61 gaacggggcg gcgtcgaggt cggccgcgag cttctcgagg tcggctttca cgtcggggta
      121 cttcatgtga cacaccggat cggtggcgca gccgttggac agcgcgtcga acgctcgctc
      181 gccggacttg accgtcgcgt cggcggaccc ggcgctgggc ggatacaccg agtcgaggac
      241 cacgctccgg atgccttcgg gataggaccg catggccgtc agcgccaggc gggtgccgta
      301 ggacacgccc cacaggttcc actcgtcgat acccatggca acccggagat cggcgaggtc
      361 ggcggcgttg gtctggctgt tgtactggtc gaggtcgtaa ccctcgtcgg tgaggcgttc
      421 gtagcagcgg gacagcgatg tgtcgaagga ctcgagctcg tccgcgaagg gcgccggatc
      481 gctcaacgcc gccaggaagg cctcctcgcg ctcggggcat tcgagcgacg gcgtggcgag
      541 tccggtgccg cgttggtcga acacgatgag atcgcgttcg gcgagcaccg ggttgcggac
      601 ccgtccggcg atgccgttgc ccagcgtgcc gatgccggga ccgccgtgca ggtagacaac
      661 cgggtcgggt tgggggctcg cggcggtgct gtggatgcgc acgacgggca gcacgacctg
      721 gttgccgtcg gggtcggccc ggttctcggg cacggtgagg gtgccgcacg tgaccgtcga
      781 gccgtccggc acctcgacga gggtgcaggg ggtgtcagcc cagatcggtt ggtactgctt
      841 cggggcggcg gtcgtggggt tggcatcgtc gccgaccgcg acgtcgttgc tcttcgagga
      901 gcacccggcg ccgaccaggg ccagagcgag cagcacgggc agggcgcggt gcgggaacat
      961 cccggattgt gtagtcgaca ggtcgcccga tcgcgccagt tgtcacacaa ctgtcgtgaa
     1021 cggtgaacag tgttcagtgc ttctggtgga gcgcctgggc ggcgatgccc tcctcgagcg
     1081 agacctcggc ggtccagccg agcaccttct cgatgagcgt cgtgtcgccg ccggtcttct
     1141 gggcgtcacc cggctggggc ggacgacggt cgagcggcac gggctggccg acggcgtcgc
     1201 cgaccatgtc gatgagttcc gcgagcgagc acgagaaacg accggcgacg ttgagcaccg
     1261 ttccgggcgc gacgtcggga gtgacaccgg cgaggtaggt ggcgttgacg acgtcgccca
     1321 cgtaggtgaa cgaccgctcc tggctaccgt cgccgaacat cgggaacggc gtctggtcga
     1381 tcgcggcgtt gatgagccgg tggtgcgcca tgtcggggcg ttgccggggg ccgtacaccg
     1441 tgaagtagcg gagtgacacc gtggggaggc cccagttggc cgagtagagg atgcacatgt
     1501 gctcaccggc gagcttcgtc acgccgtagg ggctgtacgg ctcgggccgg tcggtctcga
     1561 gggtggggta ccgctcggcg ttgccgtaga ccgacgagct ggacgcgtag acgaccctgc
     1621 cgacaccggc attgcgagcc gactcgagca cccgctgggt ggcgttgatg ttgcgactgg
     1681 cgtactggtc gaatccgtcg gaccagctca ggcgtacgcc cggctggccg gcgaggtgcc
     1741 agaccacgtc gactccggcg aagagggcgt cgaggtcggc ttcgagcagg ttctcctcga
     1801 cgagccggaa cctcgggttg gtgagtgcat cggcgatgtt ggatcgcttg agggcgacgt
     1861 cgtagtagtc ggtgaagcag tcgatgccga ccacgtcgtg gccctccgcc aggaggcggt
     1921 cgacgagggt gcttccgatg aatccggcgg cgccggtgac gatcacgttc accggagcat
     1981 tgtgacagga gatcgccgaa tgtccatcag cgggtcgccc ggctggagcg atggtttgct
     2041 ctggagcgct caggtgccaa gaactgtcac ctcagcgctc cagagcaact gagcgctccg
     2101 gacgggaccg ctggtggggg tcgcgggcta tcgggcgacg aagtacttgg cccgggggtg
     2161 gtggcagatg agggcggagg tggtctgctc gggctggtac tgccagcccg tctcctcgtt
     2221 gacctcgatg ccgatgcggc cgccgtcgag caggcgatcg atggtggcgt tgtcctcgag
     2281 gttggggcag gccgggtagc cgctcgagta gcggccgccg cggtactgct ggcggaagag
     2341 gccggcgatc gacggcccgt cctcgtccac gaagccccac tcggtgcgga tgcggtggtg
     2401 ccaatactcg gcgagcgcct cggccatctc gacgccgatc ccgtgcatca tgaggtactc
     2461 ctggtaccga tcgtcggcga acaacccggc gcagtactcc gagatgcgct cgcccatcgt
     2521 gacgatgtgg aacgcggcgt agtcgaggtc gccggtgccg gtctcggggt cgtatggacg
     2581 gaagaagtcg gcgatg
//
LOCUS       DAOSOA010000396         2582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_39953_length_2582_cov_6.034824, whole genome shotgun sequence.
ACCESSION   DAOSOA010000396 DAOSOA010000000
VERSION     DAOSOA010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39953_length_2582_cov_6.034824"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKY13_16305"
     CDS             complement(<1..492)
                     /locus_tag="PKY13_16305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT81557.1"
                     /translation="MELRDKVVVVTGAGSGIGAALVERFGRETPRVVVGVDLRGTDRT
                     VDVTDAEAVAALVRDVEAEHGPIDLYCSNAGIATGLGVDAPDEVWRRTWDVNVMGTVN
                     AARALLPSVRARGEGYLLVTASAAGLLTNLGDAPYSATKHAAVGLAEWLAITHGDDGL
                     KVSC"
     gene            complement(495..1526)
                     /locus_tag="PKY13_16310"
     CDS             complement(495..1526)
                     /locus_tag="PKY13_16310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT81558.1"
                     /translation="MHQDPDSSAPHPPLGVTLASLMTQPPSVWSSTARRATELGYSSL
                     WVAETTGPEAFAVLGSLSAEQPALSLGTGVLALQLRTPMLAVMGAATLQALRPDVDVL
                     LGVGISSPVVVGSWHGATYGDRPLAQTREYLTLARECLSGEPVTFDGDFYSCRKFRLG
                     LRAGERRPRLVIGALNAKMLALAGELADGVLLNYLPASAVPWCVEQVRAAEAAAGREP
                     GSCTVYAYVHVGVTDLDAARVPAQKDLFSYIVVDSYAAAFRRAGFGSEVDAVRAAHAA
                     RDRAAALAAVSDEMIGAIDIVGDAAHVTAAVQAYRDAGVDHPVVMPLPWGDDRLAVVD
                     ATMTAAIGG"
     gene            1689..1859
                     /locus_tag="PKY13_16315"
     CDS             1689..1859
                     /locus_tag="PKY13_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81559.1"
                     /translation="MTETRDTESISEAVALIDRGLGDISERSLVSSAEIADLLLDVRT
                     LLTRVDVLSIQN"
     gene            complement(1884..>2582)
                     /locus_tag="PKY13_16320"
     CDS             complement(1884..>2582)
                     /locus_tag="PKY13_16320"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HOT81560.1"
                     /translation="STLRQAQVGDAPLSPVWTSRSLSRAFRTLDVPLADAKAAAKRNN
                     VSVNDLFVAGATLAAGDYHRQVGRPATELRMAMPISQRADRNIGGNVFSPSQSLLPAD
                     VTDPADLLATIHEILARTKAEPAMSAANALAGVINLLPTSIVARTGYRFAGVIDFVTS
                     NLRAAPMDTFISGALMESNYPMGPLAGAAFNLTTMSFRGQLNMGLVVDTVAVEEPDRL
                     LTCLKAAYRRLLKA"
BASE COUNT          405 a          870 c          938 g          369 t
ORIGIN      
        1 acaggagacc ttgagcccgt cgtcgccgtg agtgatcgcc agccactcgg ccaggccgac
       61 ggcggcgtgt ttggtggcgc tgtacggggc atcgcccagg ttggtgagga ggcccgccgc
      121 cgacgcggtg acgagcaggt acccctcgcc acgggcgcgg accgatggca gcagcgccct
      181 ggccgcgttg acggtgccca tgacgttgac gtcccacgtc cgtcgccaca cctcgtcggg
      241 cgcgtcgacc ccgagcccgg tcgcgatacc ggcgttggag cagtacaggt cgatcgggcc
      301 gtgttcggcc tcgacgtcgc ggacgagcgc tgcgaccgcc tcggcgtcgg tcacgtcgac
      361 ggtgcgatcg gtgccgcgca ggtcgacacc gacgacgacc cgtggcgtct ctcggccgaa
      421 ccgctccacg agcgctgcgc cgatgcccga acccgcgccg gtcacgacca cgaccttgtc
      481 acgcagctcc acggtcagcc cccgattgcc gcggtcatcg tcgcgtcgac gacagcgagg
      541 cgatcgtcgc cccacggcaa cggcatcacc accgggtggt cgaccccggc gtcacggtag
      601 gcctggaccg cagcggtgac gtgggcggca tcgcccacga tgtcgatggc gccgatcatc
      661 tcgtcggaca cggcggccaa cgccgcggca cgatctcggg cagcgtgggc ggcccgcacg
      721 gcatcgacct ccgagccgaa gccggcgcgg cggaaggctg cggcatagga gtcgaccacg
      781 atgtagctga acaggtcctt ctgggccggg acccgggcgg cgtcgagatc ggtgacgccg
      841 acgtgcacgt aggcgtagac ggtgcagctt cccggctccc gtcccgcagc ggcctccgcg
      901 gcgcgaacct gctcgacgca ccacgggacc gcgctcgccg gcaggtagtt gagcagcacc
      961 ccgtcggcga gttcgccggc gagtgccagc atcttggcgt tgagcgctcc gatgacgagg
     1021 cggggacgcc gttcgcccgc tcgcaggccc aggcggaact tgcgacagga gtagaagtcg
     1081 ccgtcgaagg tgacgggctc gccactcagg cactcgcggg cgagcgtgag gtactcgcgg
     1141 gtctgggcga ggggccggtc gccgtaggtg gcaccgtgcc agctgcccac cacgaccggc
     1201 gacgagatgc ccacaccgag cagcacgtcg acgtcgggtc gcagcgcctg gagtgtggcc
     1261 gctcccatca cggcgagcat cggagtgcgg agctgcaggg ccagcacacc ggtgcccagt
     1321 gacagcgccg gctgttcggc gctcagcgat ccgagcacgg cgaacgcctc cggaccggtc
     1381 gtctcggcga cccacaacga cgagtacccg agctcggtgg cgcgtcgcgc cgtcgacgac
     1441 cacaccgacg gtggctgggt catcaggctg gcaagcgtga ccccgagtgg ggggtgcgga
     1501 gcggacgaat cagggtcctg atgcatctcg gcaccctatc tggggtagga agtggtcgcc
     1561 ggggacgtgc attcgtgtcc ggtcagttac agcagtgtga actgggtcgc agatgggcca
     1621 attgaccctc gacagccacg cagctgagcg tatgctgacg acaccaaccc aggcccagcg
     1681 gaggaacgat gaccgagacc cgtgacaccg aatccatcag tgaggccgtc gctctcatcg
     1741 atcgcggcct cggcgacatc tccgaacggt cgctcgtgtc gtcggcggag atcgccgacc
     1801 tcctgctcga cgtgcgcacg ctcctgacgc gcgtcgacgt tctgtcgatc cagaactgac
     1861 gccgtcggcg cttccggcgt cgactaggcc ttcagcagtc ggcggtacgc cgccttcagg
     1921 cacgtgagga gccggtcggg ttcctcgacg gccaccgtgt cgaccacgag gcccatgttc
     1981 agctgaccgc ggaacgacat cgtcgtgagg ttgaacgcgg caccggccaa cgggcccatc
     2041 ggatagttcg actccatcaa cgcgcccgag atgaaggtgt ccatcggggc ggcccgcagg
     2101 ttggacgtca cgaagtcgat gacgccggcg aaccggtagc cggtgcgggc gacgatcgac
     2161 gtcggcaaga ggttgatgac gccggccagg gcgttcgccg cgctcatggc cggctcggcc
     2221 ttggtgcgag ccaggatctc gtggatcgtc gcgaggaggt cggcgggatc ggtcacgtcg
     2281 gcgggcagca gggactggct gggggagaag acgttgccgc cgatgttgcg atcggcgcgc
     2341 tggctgatgg gcatggccat gcgcagctcg gtcgccggcc gaccgacctg ccggtggtag
     2401 tcgcccgcgg cgagcgtcgc cccggcgacg aacagatcgt tgaccgacac gttgttgcgc
     2461 ttggcggcgg ccttcgcgtc ggcgagtggc acgtcgaggg tgcggaaggc gcgcgacaac
     2521 gatcgtgagg tccacaccgg gctgaggggg gcgtcgccga cctgcgcctg gcggagcgtc
     2581 ga
//
LOCUS       DAOSOA010000397         2555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_40528_length_2555_cov_5.626800, whole genome shotgun sequence.
ACCESSION   DAOSOA010000397 DAOSOA010000000
VERSION     DAOSOA010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40528_length_2555_cov_5.626800"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2555)
                     /gene="valS"
                     /locus_tag="PKY13_16325"
     CDS             complement(<1..>2555)
                     /gene="valS"
                     /locus_tag="PKY13_16325"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOT81561.1"
                     /translation="EKPGLEGLEAKWGAVWAEEGTYTFDRRAERSDVFAIDTPPPTVS
                     GSLHVGHVFSYTHTDTIARFWRMRGKQVFYPMGWDDNGVPTERRVENYYGVRCDATLP
                     YEPGFTPPEKPATKRQDFVAVSRRNFIELCERLTGEDEQVFQDMWTHLGLSVDWSTMY
                     TTIGRTARRTSQRAFLRNLARGEAYSAEAPCLWDVTFQMAVSQAELEDQERPGAYHDI
                     TFPLTDSAAAGPTGEPHLMVSTTRPELLVSCVAVVAHPDDERYQPLFGTTVTTPVFGV
                     EVPIVAHHLADPEKGTGAAMICTFGDTTDVTWWRELDLPARAVIGRDGRFLHEAPRWL
                     STDGARDAYARFAGKAANGARAVMVELLTESGNLVGDIRPITHPVKFYEKGDKPLEIV
                     QSRQWYIRNGGRDLDLRARLVARGDELTWHPSYMQARYSDWVNGLNGDWLVSRQRYFG
                     VPIPVWHRLDGDGNPSYDDPLVPTEESLPIDPQTDCPPGYDESQRNQPDGFMGDPDIM
                     DTWATSSLTPQIACGWVDDDDLFSRTFPMDLRPQGHDIIRTWLFSTMVRSDAEFGTAP
                     WSNAAMSGWILDPDRKKMSKSKGNVVTPMGLLETYGSDGVRYWAASGRPGTDTAFDEK
                     EMKVGRRLAIKLLNASKFALSFGGDPDGEVHEPIDSAMLADLAALVDDATAAFEGFDY
                     ARALERTEAFFWWFCDDYLELVKLRAYGDGGPAASAANALARALSTLQRLLAPFLPFA
                     TEEVWSWWMDGSIHRTEWPSASDLRDAAGAWNPLVMGVASDVLREIRRAKSEAKVGMK
                     AEIETVTVTDSHERIAALEMVVTDVRDTGKVAELIMEPGDEFSVVTVLAPVTE"
BASE COUNT          373 a          831 c          938 g          413 t
ORIGIN      
        1 ttcggtgacg ggagcgagca ccgtgacgac ggagaactcg tcgcccggct ccatgatcag
       61 ctcggcgacc ttgccggtgt cgcggacgtc ggtgacgacc atctcgagcg ctgcgatgcg
      121 ctcgtgggaa tcggtcaccg tgacggtctc gatctcagcc ttcatgccga cctttgcctc
      181 ggacttggcg cggcggatct cccgcagcac gtcggaggcc acgcccatca cgagcgggtt
      241 ccacgcgccg gcggcgtcgc ggaggtcgga cgccgacggc cactcggtgc ggtggatcga
      301 gccgtccatc caccacgacc acacctcttc ggtcgcgaac gggaggaacg gcgccaggag
      361 gcgctgcagg gtggacagcg ctcgcgccag cgcgttcgct gccgaggcgg cgggaccgcc
      421 gtcgccgtag gcacgcagct tcaccaactc gaggtagtcg tcgcagaacc accagaagaa
      481 cgcctcggtg cgctcgagtg ctcgggcgta gtcgaagccc tcgaacgccg cggtggcgtc
      541 gtcgacgaga gcggccaggt cggcgagcat cgccgagtcg atcggctcgt ggacctcgcc
      601 gtcggggtcg ccgccgaacg agagcgcgaa cttcgatgcg ttgaggagct tgatggcgag
      661 gcgacgcccg accttcatct ccttctcgtc gaacgccgtg tcggtgcccg gtcgacccga
      721 tgccgcccag tagcggacgc cgtcggaccc gtaggtctcg agcaggccca tcggtgtgac
      781 gacgttgccc ttcgacttcg acatcttctt gcggtcgggg tcgaggatcc accccgacat
      841 cgcggcgttg ctccacggcg cggtaccgaa ctccgcgtcg gagcggacca tcgtcgagaa
      901 cagccacgtg cggatgatgt cgtggccctg cggacgcagg tccatcggga aggtgcggga
      961 gaacaggtcg tcgtcgtcga cccacccgca cgcgatctgc ggcgtgagcg acgaggtggc
     1021 ccacgtgtcc atgatgtcgg ggtcgcccat gaagccgtcg ggctggttgc gctgggactc
     1081 gtcgtaaccc ggagggcagt cggtctgcgg gtcgatcgga agggactcct cggtggggac
     1141 cagcgggtcg tcgtagctgg ggttgccgtc gccgtcgagc cggtgccaca ccgggatcgg
     1201 gacaccgaag taccgctggc ggctgacgag ccaatcgccg ttgaggccgt tgacccagtc
     1261 gctgtaccgc gcctgcatgt acgacggatg ccacgtgagc tcgtcgccgc gggcgacgag
     1321 tcgggcgcgc aggtcgaggt cgcggccgcc gttgcggatg taccactggc gcgactggac
     1381 gatctcgagc ggcttgtcgc ccttctcgta gaacttgacc gggtgcgtga tcgggcggat
     1441 gtcgccgacg aggttgcccg actcggtcag cagttcgacc atgacggctc gggcgccgtt
     1501 cgccgccttg ccggcgaagc gggcgtaggc gtcgcgggcg ccgtcggtgg acagccaccg
     1561 cggtgcctcg tgcaggaagc gaccgtcgcg accgatgacg gcgcgggccg gcaggtcgag
     1621 ctcgcgccac cacgtgacgt cggtggtgtc gccgaacgtg cagatcatcg ccgcgcccgt
     1681 gcccttctcg ggatcggcga gatggtgggc gacgatcggg acctcgaccc cgaacaccgg
     1741 cgttgtcacc gtcgtgccga acagcggctg gtagcgctcg tcgtcggggt gggcgacgac
     1801 cgcgacgcag ctgacgagca gctcgggacg cgtcgtcgac accatgaggt gtggttcgcc
     1861 ggtcgggccg gcggcggcgc tgtcggtgag cgggaacgtg atgtcgtggt aggcgcccgg
     1921 ccgctcctga tcctcgagtt cggcctgact gaccgccatc tggaacgtga cgtcccagag
     1981 gcagggcgcc tcggccgagt aggcctcgcc gcgggcgagg ttgcggagga aggcgcgctg
     2041 actcgtgcgg cgggcggtgc ggccgatcgt cgtgtacatg gtcgaccagt cgaccgacag
     2101 gcccaggtgg gtccacatgt cctggaagac ctgctcgtcc tcgccggtca gccgttcgca
     2161 cagctcgatg aagttgcggc ggctcacggc gacgaagtcc tgtcgcttgg tggccggctt
     2221 ctccggcggg gtgaacccgg gctcataggg gagcgtggca tcgcaccgca ccccgtagta
     2281 gttctcgacg cgacgctcgg tgggaacgcc gttgtcgtcc caacccatcg ggtagaagac
     2341 ctgcttgcca cgcatgcgcc agaagcgggc gatcgtgtcg gtgtgggtgt agctgaagac
     2401 gtggccgacg tggagggagc ccgagaccgt gggcggcggt gtgtcgatcg cgaagacgtc
     2461 ggaacgttcg gccctgcggt cgaacgtgta cgtgccctcc tcggcccata ccgcgcccca
     2521 cttggcctcg aggccctcga ggcccggctt ctcgg
//
LOCUS       DAOSOA010000398         2544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_40758_length_2544_cov_5.265569, whole genome shotgun sequence.
ACCESSION   DAOSOA010000398 DAOSOA010000000
VERSION     DAOSOA010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40758_length_2544_cov_5.265569"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKY13_16330"
     CDS             complement(<1..414)
                     /locus_tag="PKY13_16330"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOT81562.1"
                     /translation="MLVGLALIASASACVPDGPSGGAAVGAQCTSPPQIVAGASLFGC
                     DLAGLDLHGAVLTGADLRGALLGGANLAGADLRNANLTGADLSGANLTGADLAGAVLA
                     GALLLGTILALANLTGASFDFGTVFGFGAGDPAPPA"
     gene            549..2348
                     /locus_tag="PKY13_16335"
     CDS             549..2348
                     /locus_tag="PKY13_16335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOT81563.1"
                     /translation="MTDTASDTSSVDDIAWDLEPLLPTPGADGVTELLDAAAAEVDHL
                     ETYRGRLVDLSADELADFFEHLADVIDKLGRAGNYTSLKFSVATDDPEVGALMQKTQE
                     RSTIIGSRLIFVELELAQLSDERAAELIADPQLANVAHHLEGILRYKDHLLSEPEEVV
                     LSEKSVAGASAWSRLYDEQSSVITVKQGDDEVPLMAALAGLQNPDATKRAEIAAAITD
                     GLQPGIRTRAFIYNTLLSDKSVDDRLRKYPTWISARNLSNEASDESVQALIEAVVGRY
                     EIPQRWYALKAKMLGVPKLKDWDRMAPVVEDTAEFGWDEAKSLVDDAYRSFSPEIADV
                     VQQFFQNDWIDAPMRPAKRGGAFCAYTVPSHHPYLLLNWTSQRRDVLTLAHELGHGVH
                     AYLARPQGIFHQTTPLTLAETASVFGETVTFGKLLDGVTDPAERLPLLAEHLEDQIAT
                     VFRQVAMNRFEDAVHTERREVGELSVERFGELWGESQTAMLGDAVEVSEGYKTWWSYV
                     PHFIHTPGYVYAYAYGQLLALSVYARYVEEGPSFVPRYLELLAAGGSKSPQDLGKIVG
                     VDLGDPGFWDGGLDIIEQLLVDTEQAAADAGRV"
BASE COUNT          419 a          923 c          829 g          373 t
ORIGIN      
        1 ggccggtggt gccggatcgc ccgccccgaa tccgaacacg gtcccgaagt cgaagctcgc
       61 tccggtgaga ttggcgaggg cgaggatcgt cccgaggagg agggcgccgg cgagcacggc
      121 acctgcgagg tcggctcccg tcagattcgc tccggacagg tcggcgccgg tgaggttcgc
      181 gttgcggagg tcggcgcctg cgagattggc gccgccgagc agcgcgcccc ggaggtcggc
      241 gcccgtcagc acggcaccgt ggagatcgag gccggcgaga tcgcagccga acaggctcgc
      301 tccggcgacg atctgcgggg ggctggtgca ctgagccccg accgcagcgc ctcccgacgg
      361 tccgtcgggg acacatgcgc tcgccgaggc gatgagcgcg aggcccacca acacgccaac
      421 gatcctgctg ccgaaccgac ccatgccaac ctcccgcacc ggcgcacccc acgtcggtga
      481 cacgatgtta ctgacttcga gctcgcaggc aaccaccgtt gtggccggct cgcacctagc
      541 ctgtcgccat gaccgatacc gcttccgaca ccagctccgt cgacgacatc gcctgggacc
      601 tcgagcccct cctgcccacg cccggcgccg acggcgtcac cgagctcctc gatgccgccg
      661 cggccgaggt cgaccacctc gagacctatc gcggtcggct cgtcgacctg agcgcggatg
      721 aactggccga cttcttcgag cacctcgccg acgtcatcga caaactggga cgcgccggca
      781 actacacgtc gctgaagttc tcggtggcca ccgacgatcc cgaggtgggc gcgctcatgc
      841 agaagacgca ggagcggtcg acgatcatcg gctcgcgact gatcttcgtg gaactcgagc
      901 tggcacagct gtccgacgag cgggcggccg agctgatcgc cgacccccag ctcgccaacg
      961 tcgcccacca cctcgagggc atcctccgct acaaggacca cctgctcagc gagcccgaag
     1021 aggtcgtgct cagcgagaaa tcggtcgccg gcgcctccgc gtggtcacgc ctctacgacg
     1081 agcagtcgag cgtgatcacc gtcaagcagg gcgacgacga ggtgccgctc atggcggccc
     1141 tcgccggtct ccagaacccc gacgccacca agcgcgccga gatcgcggcg gcgatcaccg
     1201 acgggctgca gccgggcatc cgcacccgtg ccttcatcta caacacactc ctgtcggaca
     1261 agtccgtcga cgaccgcctg cgcaagtatc cgacgtggat ctcggcgcgg aacctgtcca
     1321 acgaggcgtc cgacgagtcg gtgcaggcgc tgatcgaggc cgtcgtcggg cgctacgaga
     1381 tcccccagcg gtggtacgcg ctgaaggcga agatgctcgg cgtgcccaag ttgaaggact
     1441 gggaccgcat ggcgccggtg gtggaggaca ccgccgagtt cggttgggac gaggcgaagt
     1501 cactcgtcga cgacgcgtac cgctcgttct ccccggagat cgccgatgtc gtccagcagt
     1561 tcttccagaa cgactggatc gacgcgccga tgcgacccgc caagcgtggg ggagcgttct
     1621 gcgcctacac cgtgccctca caccacccgt acctgctgtt gaactggacg tcgcagcgcc
     1681 gcgacgtcct gacactcgcc cacgagctcg gtcacggggt gcacgcctac ctggcccggc
     1741 cccagggcat cttccatcag accaccccgt tgaccctcgc cgagacggca tcggtgttcg
     1801 gcgagaccgt cacgttcggc aagctgctcg acggcgtgac cgatccggcc gaacgcctgc
     1861 cactgctcgc cgagcacctc gaggaccaga tcgccaccgt gttccgccag gtggccatga
     1921 accggttcga ggacgccgtg cacaccgagc gtcgcgaggt cggcgagctg tcggtcgaac
     1981 gcttcggtga actgtggggc gagagccaga cggcgatgct cggcgacgcc gtcgaggtca
     2041 gcgaggggta caagacctgg tggtcgtacg tgcctcactt catccacacc ccgggctacg
     2101 tgtacgccta cgcctacggg cagctgctgg cgctgtcggt gtacgcccgc tacgtcgagg
     2161 agggcccgtc gttcgtgccc cgctacctcg agctgctcgc cgcgggcggt tccaagtcac
     2221 cccaggacct cggcaagatc gtcggggtcg atctcggcga cccgggattc tgggacggcg
     2281 gtctcgacat catcgagcag cttctcgtcg acaccgagca ggcggctgcc gacgccgggc
     2341 gcgtctaacg aactaatcct caacccagtg ggtcggcacg ccgacccact ggggcatgtt
     2401 cggttcgttc tccctccgac gcgcggtcac cgctgcgatc gccgtcgccg cgctcggctc
     2461 ggcctgcgcc gccccgccgc ccgtcggcaa gttcgccgac ctcgaccagc gggtcgagcg
     2521 catcgacgac ctcacctacg gggc
//
LOCUS       DAOSOA010000399         2539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_40856_length_2539_cov_6.103462, whole genome shotgun sequence.
ACCESSION   DAOSOA010000399 DAOSOA010000000
VERSION     DAOSOA010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40856_length_2539_cov_6.103462"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PKY13_16340"
     CDS             complement(<1..1373)
                     /locus_tag="PKY13_16340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT81564.1"
                     /translation="MLSPRTHSNLPAVVGGGVAGLVAVGASLDAFGEYSSAAFGAVGV
                     TAVITLVRGAFLMPKGARHPWVMLAIAVFLVMVAAALRDSYHTVGDLTANRSLVPDLA
                     SLPGYVLLAVALGCAVRIRRRDVDGGLDAVLDGCLAGLAALALAWVFLIEPALARSTA
                     TIGVRLSIVAYPPLSVVFVVMTCQIAFARGAKRGASILGVVVAMVFLLIGDVFFTIGD
                     AKVADFGPASWLLPYAIGYGAIVFFCLHPSFVEFAVPLPADNAPASPARLAFVSLALT
                     TPAMVSVTQHDMATADRVVLATIIFVLTATATWRVVRALQHHARSEKALLYQATHDLL
                     TGSLNRSGLLDELSELAEAGIPHAVLFVDMDRFKLVNDSFGHSFGDDLLIAVAERLRA
                     SSRRGDVVARIGGDEFVVVTTYDAGIDQVVRTAEELRQSFSRPFLIRDTEIPVSISVG
                     IATSDLDG"
     gene            complement(1493..>2539)
                     /locus_tag="PKY13_16345"
     CDS             complement(1493..>2539)
                     /locus_tag="PKY13_16345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT81565.1"
                     /translation="ATQIIGDKSDPAVLRELEKSGDDRFDRGLWAALADAGLLGISLP
                     ESAGGAGLGLIEAGCVIRAAAYHAAAVPVWETLGLGAPAIAEFADEATKNDWLGKVAS
                     GDAVLTAAWHDELGDPLDPQLVTFDGSTVTGVKVCVPAGNVADAAVVSVKGANGPALV
                     LVDLSASGVTRTAETTTAGTPEASLEFSSTPATLLAEGDEALTWAFDRAVATQCTAAL
                     GTAEAALALTAEYTKERKQFDVPIATFQAVGHRAADTFIDTQTIRLTAWHALWRLSTG
                     LSATEEVATAKYWAAYSGHRVSLASAHLHGGVGVDRDYPLHRHFIAAKQHELQLGGAT
                     QALLRLGREIAANA"
BASE COUNT          421 a          842 c          939 g          337 t
ORIGIN      
        1 ccatcgaggt cggacgtggc gataccgacg ctgatcgaca ccgggatctc ggtgtcacgg
       61 atgaggaacg ggcgggagaa cgactgccgg agctcctccg cggtgcgcac cacctgatcg
      121 atgccggcgt cgtaggtcgt gaccacgacg aactcgtcgc cgccgatccg agcgacgaca
      181 tcgccgcgtc gcgacgatgc ccgtaggcgc tcggccacgg cgatcagcag atcgtcaccg
      241 aagctgtggc caaagctgtc gttcaccagc ttgaaccggt ccatgtccac gaacagcacc
      301 gcgtggggga tgcccgcctc ggccagctcc gagagctcgt cgaggagacc ggagcggttg
      361 agcgatccgg tgaggaggtc gtgggtcgcc tggtagagca gggccttctc ggaccgggcg
      421 tgatgctgca gggcacgcac cacccgccac gtggcggtcg cggtcaggac gaagatgatg
      481 gttgccagca cgacccggtc ggcggtcgcc atgtcgtgtt gtgtcaccga caccatcgcc
      541 ggtgtggtga gcgccaacga cacgaaggca agtcgggcgg gcgacgccgg cgcgttgtcg
      601 gccgggagcg gcaccgcgaa ctcgacgaag ctcgggtgca ggcagaagaa cacgatggcg
      661 ccgtagccga tggcgtacgg caggagccac gacgccggcc cgaagtcggc gaccttcgcg
      721 tcgccgatcg tgaagaacac gtcaccgatc aggaggaaca ccatggcgac gaccacgccg
      781 aggatcgacg cgccccgctt ggcgccgcgg gcgaaggcga tctgacaggt catgacgacg
      841 aagacgaccg acagcggcgg gtaggcgacg atcgagaggc ggacgccaat ggtcgccgtc
      901 gaccgcgcca gtgccggttc gatgaggaac acccacgcga gcgcgagggc ggcgaggccg
      961 gcgaggcagc cgtcgagcac cgcgtcgagt ccgccgtcca cgtcgcgacg gcggatgcga
     1021 acggcgcagc ccagggccac cgccaacagc acgtagccgg gcaacgaggc gaggtcgggg
     1081 acgagcgaac ggttggccgt cagatcaccg accgtgtggt acgagtcgcg caatgccgcc
     1141 gcgaccatca ccaggaacac ggcgatcgcc agcatcaccc aggggtgacg ggcgcccttg
     1201 ggcatcagga acgcgccgcg cacgagcgtg atgaccgcgg tcactccgac ggcgccgaag
     1261 gccgccgacg agtactcacc aaaggcatcg aggctcgcgc cgacggccac gaggccggcg
     1321 acacctccgc cgacgacggc ggggagattc gaatgtgtgc ggggagacag catcaggagt
     1381 cgatccggtc gaggattgat cactccttgt atcgaccgcc ccttcgcccg ggttgagcat
     1441 tcctacccgc cccgggtggt gtcgcccgac tcccccgccg gtcgggccgc cgtcaggcgt
     1501 tggcggcgat ctcccggccg agtcgcagga gggcctgggt ggccccaccg agctgcagct
     1561 cgtgctgctt cgcggcgatg aagtggcggt gcagcgggta gtcgcggtcg acaccgacgc
     1621 cgccgtggag gtgcgccgac gccagcgata cccggtgacc gctgtaggcg gcccagtact
     1681 tggcggtggc gacctcttcg gtggccgaca ggcccgtcga gagccgccag agcgcgtgcc
     1741 acgcagtgag gcgaatcgtc tgcgtgtcga tgaatgtgtc cgcggcgcgg tgaccgacgg
     1801 cctggaaggt ggcgatcggg acgtcgaact gcttgcgttc cttggtgtac tcggcggtga
     1861 gggccagtgc ggcctccgcc gttccgagcg ccgcggtgca ctgtgtggcg acggcacggt
     1921 cgaacgccca cgtgagggcc tcatcgccct cggcgaggag tgtcgccggt gtcgacgaga
     1981 actcgagcga cgcctcgggg gtaccagcgg tcgtcgtctc ggccgtgcgg gtgacaccgc
     2041 tcgccgagag gtcgaccagc acgagcgccg ggccgttcgc acccttgacc gacaccacag
     2101 cggcgtcggc aacgttgccc gccggcacgc acaccttgac gccggtcacg gtgctgccgt
     2161 cgaaggtcac gagctgcgga tcgagcgggt cgccgagctc gtcgtgccag gcggcggtga
     2221 gcactgcatc gcccgacgcg accttgccga gccagtcgtt cttggtggcc tcgtcggcga
     2281 actcggcaat ggcgggggcg ccgagaccga gcgtctccca gacgggtacg gcggcggcgt
     2341 ggtaggcggc ggcgcggatc acgcagccgg cctcgatcag gccgaggccc gctcccccgg
     2401 ccgattccgg cagggagatg ccgagcaggc cggcgtcggc gagcgcggcc cagagcccgc
     2461 ggtcgaatcg gtcgtcaccc gacttctcca gctcgcggag gacggcgggg tcggacttgt
     2521 cgccgatgat ctgggtggc
//
LOCUS       DAOSOA010000400         2537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_40906_length_2537_cov_4.074134, whole genome shotgun sequence.
ACCESSION   DAOSOA010000400 DAOSOA010000000
VERSION     DAOSOA010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40906_length_2537_cov_4.074134"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKY13_16350"
     CDS             complement(<1..610)
                     /locus_tag="PKY13_16350"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT81566.1"
                     /translation="MVRTPVGQVADSALAGAGPARCDLPSAGADFEMVAPEAVGVDGA
                     AVRATIDELSTPMTRSLRIYRHGCLIGQSRRDLTSAHEPAPLFSMTKSVVALAVGRAV
                     TLGAVELDSPIGRYLPDLDAAHAAITVRQLLTQTSGLRFGWVNDLLGSTEDSVGEAMS
                     MPLVHEPGAVFEYAQTTVTTLAFVVERAVGQDFQAFVSEQLFE"
     gene            complement(706..1620)
                     /locus_tag="PKY13_16355"
     CDS             complement(706..1620)
                     /locus_tag="PKY13_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOT81567.1"
                     /translation="MLNSEFRVGDEVAEALAMGRPVVALESTILSNLGLPSPYNRECL
                     DRCDAAVRGAGAVPAITAVLDGCATVGLTEPEIDRVLLGTNKVSARDVAVAVGLGWDI
                     GVTTVAASVMLAARAGIEVFATGGIGGVHRGAELTGDVSADLGALARYPVVTVTAGAK
                     AFLDLARTVEWFDTLGVPLLGYGTDEFPAFYSRTSGVAVPHRVETATEVASIVGAMRR
                     AGERGGVVVANPIPESAEIPSHEIDLVIAAALAEAAAAGVTGPGVTPFVLEAISVATA
                     GRSIPANLALAEHNAGVAADIAVALAEG"
     gene            complement(1607..2470)
                     /locus_tag="PKY13_16360"
     CDS             complement(1607..2470)
                     /locus_tag="PKY13_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOT81568.1"
                     /translation="MIVSVGDLATDLVVRLGSSEIDPVSDTPAVVSLHRGGAAANVGA
                     VSARLGRAARFVGALGADDLSVAAASALRSLGVEVCGPPARRGWSIVVLVGPDGGRRF
                     LTDTGDVDPWPAADPAWLDGAARLHLSGYAVCNERSSAACLELATLAAELEIPRSLDV
                     SSASVVRGWGVERFADDVRSIAPDVVKANEDEDELLPPGLAAAIVVTRGSEPTVLRTA
                     DGRYETVEVPPRAVVDTTGAGDAFVAGFLAAWDGGAAGVDAVVAGHAAAARVVTGPGA
                     DWWETGAHVEQ"
BASE COUNT          430 a          944 c          853 g          310 t
ORIGIN      
        1 gttcgaacaa ctgctcggac acgaaggcct ggaagtcctg ccccacagcc cgctccacca
       61 cgaacgccag tgtcgtgacc gtcgtctgcg cgtactcgaa caccgcgccg ggctcgtgga
      121 cgagcggcat cgacatcgcc tcgcccaccg agtcctcggt cgagcccagg aggtcgttga
      181 cccatccgaa ccggaggccc gacgtctggg tcaggagctg ccggaccgtg atcgcggcgt
      241 gcgctgcgtc gagatcgggc agatagcgcc cgatcgggga gtccagctcg acggcaccca
      301 aggtcactgc tcgacctact gccaacgcga cgacgctctt ggtcatcgag aagagcggcg
      361 ccggctcgtg ggccgacgtg agatctcgcc ggctctgacc gatgaggcac ccgtgccggt
      421 agatccgcag cgaccgagtc atcggggtcg agagctcgtc gatcgtggcc cgcaccgcag
      481 cgccgtcgac acccaccgcc tccggagcga ccatctcgaa gtcggcaccg gcggagggaa
      541 gatcgcaccg ggcgggcccg gcgcccgcca gggcactgtc ggcgacctgc ccgacgggag
      601 tccgcaccac cgacgggccc aggtccgcgc acgaagagag cagcgccgcg acgcacaccc
      661 cgacaacgag cctccccttg atgacggcga gcgtaccggc ggggttcagc cctcggcgag
      721 tgcgacggcg atgtcggcag cgacgcctgc gttgtgctcg gcgagtgcga ggttggcggg
      781 aatgctgcgg cccgcggtcg ccacggagat ggcctcgagc acgaacggcg tcacccccgg
      841 gccggtcact cccgctgcgg ctgcctcggc gagtgccgcc gcgatcacca ggtcgatctc
      901 gtgggacggg atctccgccg actccggaat cggattcgcc acgaccactc ccccacgctc
      961 gcccgcacgg cgcatcgctc ccacgatcga cgcaacctcg gtcgcggtct cgacccggtg
     1021 cggaaccgcc acccctgatg tccggctgta gaaggcggga aactcatcgg tgccatagcc
     1081 gagcagcgga acaccaaggg tgtcgaacca ctcgacggtg cgggccaggt cgaggaaggc
     1141 cttcgctccg gccgtgacgg tgacgacggg gtaccgggcg agcgcgccga ggtcggcaga
     1201 gacgtcaccg gtcagctcgg ccccccgatg caccccgccg atgccgccgg tggcgaacac
     1261 ctcgatgccg gcgcgggccg ccagcatgac cgatgcagca accgtggtca cgccgatgtc
     1321 ccacccgaga ccgaccgcca ccgccacatc gcgcgccgag accttgttcg tgccgagcag
     1381 cacccggtcg atctcgggct ccgtgaggcc gaccgtggcg cagccatcga gcacggccgt
     1441 aatggccggg acggcgccgg cgccgcgtac ggcggcgtcg cagcgatcga gacactcacg
     1501 gttgtacggc gacggcaagc cgaggttcga caggatcgtc gactccaacg ccacgacggg
     1561 ccggcccatc gccagcgcct cggcaacctc gtcgccgacg cggaattcac tgttcaacat
     1621 gggctccagt ctcccaccag tctgcgcccg gcccggtcac gacccgtgcg gccgcagcgt
     1681 gtccggccac aacggcgtcg acgcctgctg cgccgccgtc ccaagccgcc aggaaccctg
     1741 cgacgaaggc atcacccgcc cccgtcgtgt cgaccacagc tcgaggagga acctccaccg
     1801 tctcgtaccg gccgtcggcg gttcgcagca cggtgggttc cgaaccgcgg gtgacgacga
     1861 tcgcggcggc gaggcccggt ggcaggagct cgtcctcgtc ctcattcgcc ttgacgacgt
     1921 ccggcgcgat cgagcgcaca tcgtcggcga agcgttcgac tccccaccct cgtacgaccg
     1981 atgccgaact gacgtccaac gaacgcggga tctccagctc ggcggcgagc gtcgcgagct
     2041 cgaggcaggc cgcggacgag cgctcgttgc acaccgcgta cccgctgaga tgcaggcgag
     2101 ccgcaccgtc gagccatgcc ggatcagcgg ccggccacgg gtcgacgtcg ccggtgtcgg
     2161 tgaggaaccg gcggcctccg tcagggccga ccaacaccac gatcgaccaa ccacgtcgcg
     2221 ccggcggccc gcagacctcg acgcccagcg agcgaagtgc agatgccgcg gcgaccgaca
     2281 ggtcgtccgc gcccagcgcg ccgacgaacc gtgcggcgcg gccgagacgt gccgacaccg
     2341 caccgacgtt ggctgccgcg ccgccacggt gaaggctgac gaccgccggg gtgtcggaga
     2401 ccgggtcgat ctctgaggag ccgagccgga cgaccaggtc cgtggcgagg tcaccgacgc
     2461 tgacgatcat cgctgccacc gtacgcgaag agcccccgct gccgaagcaa cgggggctct
     2521 caccaaacca ggaacaa
//
LOCUS       DAOSOA010000401         2533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_41002_length_2533_cov_6.442292, whole genome shotgun sequence.
ACCESSION   DAOSOA010000401 DAOSOA010000000
VERSION     DAOSOA010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41002_length_2533_cov_6.442292"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PKY13_16365"
     CDS             <1..398
                     /locus_tag="PKY13_16365"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT81569.1"
                     /translation="FVIVVLLVGAVAAFLGDVAGATTYGLVLAAVAVLVLAGWWVFSG
                     LVFRAYGWKLTDGTVELRHGVVVQRHQVLPRARVQNVTKEAGPVSRAFGLASITVHSA
                     GANTPNITVPDLTVEVGDRLRTNLLPAAR"
     gene            395..1894
                     /locus_tag="PKY13_16370"
     CDS             395..1894
                     /locus_tag="PKY13_16370"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT81570.1"
                     /translation="MIATNPAPSPVEREFGEWRRVDPRTPLIYALPVVVFGGWYVLVT
                     MGAASRGDRIGAAIEGLIVPLLGVVGVVVRYLTGRYAIVDGALRWKVGLVVHKATDIG
                     LDRIQDVEVTRPLLTRIVGLAALNVSSAGGAGEIKLQYLDHQVAERLGAHLQHLISVE
                     RHAAAAQGYSTAPTVGVPPVGRAADATPLHRVETPELVAWAAYRLWPVVPVFVAAVAA
                     AFLVDWKAVLPLVPTLPFVLFGMVKPLLDRTNLTVSIDATSLFTSEGLTTVRRTSTQR
                     ERVQLVFAHQLWLQRLRGSETVRYASADVTLDRKEGAVREELALNVPADTWGTMAATV
                     AHRQVVGPAWLRRKPSAAATASRVRCSGAGSAVGVVGAIVALAFREPLVAAVVLLVAS
                     LVGLGVGVWRGGRRVAVEGWVVTPQDLLIRHGLLRRTTLLLFAEKTQAIDVVSGPLQR
                     RLGLVTVVVDVAPPGRASSVIIRDVSVAEADELRAALTASGSIPLPNGV"
     gene            2037..>2533
                     /locus_tag="PKY13_16375"
     CDS             2037..>2533
                     /locus_tag="PKY13_16375"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81571.1"
                     /translation="MAFTKSERLKIIVWGTGYVGKMVLRDLIGHPLFELVGVIVNNPE
                     KDGVDVGTLIGRDPIGLACSTDIDEVLSRDAVAVAHFGPTAAHPGANFKSAIASMRAG
                     KHWVSTAMTPWVYPPVCGPGEIDSVVAVCEETGKSCFTTGIDPGFANDLFPMTLMGLS
                     GRVDSV"
BASE COUNT          328 a          869 c          906 g          430 t
ORIGIN      
        1 gattcgtgat cgtggtgctc ctggtcgggg ccgtcgccgc gttcctcggc gacgtcgccg
       61 gcgccacgac ctacggtttg gtgctggctg ccgttgcggt gctggtgctg gcggggtggt
      121 gggtgttctc ggggctggtg ttccgggcgt acggctggaa gctcaccgac ggcacggtcg
      181 agctccggca cggcgttgtc gtccaacgac accaggtcct gccgcgggcc cgggtccaga
      241 acgtgacgaa ggaagccggc ccggtgtcgc gggcgttcgg cctggcatcg atcaccgtcc
      301 attcggccgg cgcgaacacg cccaacatca cggtgcctga cctcaccgtc gaggtgggcg
      361 atcgcctgcg caccaatctc ctcccggcgg cgcgttgatc gcgacgaacc ctgccccctc
      421 gcccgtcgag cgcgagttcg gcgaatggcg tcgcgtcgac ccgcggacgc cgctgatcta
      481 tgcgctgccg gtcgttgtgt tcggcggctg gtacgtgctc gtgacgatgg gcgctgcgag
      541 ccgcggtgac cgcatcggag cggcgatcga gggactgatc gtgccgctgt tgggcgtggt
      601 cggggtggtc gtccgctacc tcaccggtcg gtacgcgatc gtcgacggcg cgttgcgttg
      661 gaaggtcggc ctcgtcgtgc acaaggcgac cgacatcggc ctcgaccgca tccaggacgt
      721 cgaggtgacg cgcccgttgc tgacccgcat cgtcgggctg gccgcgctga acgtgagctc
      781 cgccggcggg gccggggaga tcaaactcca gtatctcgat caccaggtgg ccgaacggct
      841 cggcgcccat ctccagcacc tgatctccgt ggagcggcat gccgccgccg cgcagggcta
      901 cagcaccgcg cccacggtgg gcgttccgcc cgtcggtcgg gccgccgatg cgacgccgct
      961 ccatcgagtc gagacccccg agctcgtcgc gtgggccgcg taccggttgt ggcccgtcgt
     1021 gccggtgttc gtcgcggccg tcgccgcagc gttcctcgtc gactggaaag cggtcctgcc
     1081 gttggtcccg accctgccgt tcgtgctgtt cggcatggtg aagccgttac tcgaccgcac
     1141 caacctcacg gtcagcatcg acgcgacgtc gctgttcacg tccgagggcc tgacgacggt
     1201 gcgccgaacg tcgacgcagc gggagcgggt ccagctcgtg ttcgcgcacc aattgtggtt
     1261 gcaacgactc cgtggctccg agacggtgcg gtacgcctcg gccgacgtca cgctcgaccg
     1321 caaggagggc gccgtccgcg aggagctggc cctcaacgtc ccggccgaca cctggggcac
     1381 gatggcagcg accgtcgccc accgtcaggt cgtcggtccg gcctggctgc gccggaagcc
     1441 ctcggcggcc gccacggcga gccgtgtgcg gtgcagcggt gccggctcgg ccgtcggcgt
     1501 cgtcggtgca atcgtggcgc tggcgttccg cgagccgctg gtcgcggccg tcgtgctgct
     1561 cgtcgcctcg ctcgtcgggc tgggtgtcgg ggtgtggcga ggagggcgtc gggtcgcggt
     1621 cgaaggttgg gtcgtgacgc cgcaggacct cctcatccgc cacggtctcc tgcgtcgcac
     1681 gacgctcctc ctcttcgccg agaagacgca ggcgatcgac gtcgtgtcgg gtccgttgca
     1741 gcgccggctc ggtctggtca ctgtcgtcgt cgatgtcgca ccgcccggtc gagcgagcag
     1801 cgtgattatc cgcgacgtct cggtcgccga ggcggacgag ttgcgagcag cgctcaccgc
     1861 ctcgggcagc atcccgctcc cgaacggggt gtgagcccga gcggaggtgg tcgaggctgc
     1921 tcgaggcgtg aggtgtatca atacaactcg ccgcgtgcac cttcgacgcc tgccggagtg
     1981 ccccctacga tcacccacgg tcgcccacgc ggggcgccgt ctgaaggggg aacgtcgtgg
     2041 cattcaccaa gtcggagcgg ctcaagatca tcgtctgggg caccgggtac gtcggaaaga
     2101 tggtgctgcg cgacctgatc gggcacccgc tgttcgagct ggtgggtgtg atcgtgaaca
     2161 accccgagaa ggacggcgtc gacgtcggca ccctcatcgg ccgtgacccg atcggcctcg
     2221 cgtgcagcac cgacatcgac gaggtgctgt cgcgtgacgc ggtcgcggtc gcccacttcg
     2281 gcccgacggc ggcccacccc ggcgccaact tcaagagcgc gatcgcatcg atgcgagccg
     2341 gcaagcactg ggtgtccacc gcgatgacgc cctgggtgta cccgccggtg tgcggccccg
     2401 gcgagatcga ctccgtcgtt gccgtctgcg aggagaccgg caagagctgc ttcaccaccg
     2461 gcatcgatcc cggcttcgcc aacgacctgt tcccgatgac gctcatgggc ctgtcgggtc
     2521 gcgtcgactc ggt
//
LOCUS       DAOSOA010000402         2527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_41127_length_2527_cov_8.953074, whole genome shotgun sequence.
ACCESSION   DAOSOA010000402 DAOSOA010000000
VERSION     DAOSOA010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41127_length_2527_cov_8.953074"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            547..1221
                     /locus_tag="PKY13_16380"
     CDS             547..1221
                     /locus_tag="PKY13_16380"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81572.1"
                     /translation="MTDLASIEARAARHAALGDPIRLAIVDELAVSDRAPIELRGLVG
                     LESNLLAHHLDVLEEVGLISRSRSSGDGRRRYVHLIRAALDGLTPGRHIPIGPALFIC
                     TANSARSQLAAALWRELSGESGDSAGTHPVDRVNPGAVAAASRAGLDVAGATPRELTD
                     GERLPGLVVTVCDRAHEELAGDETWLHWSIPDPVPLGTKAAFDATVAELRDRIESILD
                     ERDLAS"
     gene            1218..1970
                     /locus_tag="PKY13_16385"
     CDS             1218..1970
                     /locus_tag="PKY13_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOT81573.1"
                     /translation="MIDPLHPSDAELSVQPETGTGQLRLDFARRLTAEALGTALLIIA
                     VIGSGIMASRLSPDDIGLQLLENAAATAGALIGLILIFGAVSGAHFNPVVTLVDRLLG
                     TVTTRDAGLYVVAQTIGGCVGTVLANLMFELPAVELSTTTRSSGALWLSEAIATVGLL
                     LVIHGCVRTGKASAVPFAVGLWIGGAYWFTSSTSFANPAVTIARTLSDTFAGIRPSSA
                     PMFIVMQVIGAGIAYALIRFLYPHNLSETPHE"
     gene            1963..2385
                     /locus_tag="PKY13_16390"
     CDS             1963..2385
                     /locus_tag="PKY13_16390"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOT81574.1"
                     /translation="MSEHRPEVLFVCIHNAGRSQMAAALLAHHGGDQVVVRSAGTAPA
                     ETVNPAVVEAMAELGIDLHASGAFPKRLDDAAVEASDVVITMGCGDECPFYPGKRYLD
                     WALDDPAGQGVDAVRPIRDEIDRRIRELLAELLPSATP"
BASE COUNT          402 a          954 c          761 g          410 t
ORIGIN      
        1 ccaccggcct cgccaacatc aacgtcgacc tcaccgacaa cggcggcacc gccaacggcg
       61 ccaacaacac cgccatcaac cgcaccttcc agatcaccgt caacccatga gcaagcagca
      121 gactcgtccc aacctgtttg ccggggacga cgttggctcg gccgaagatg catggggtcc
      181 cacgatcgca gcgtccgcgt tctgtcggcc gtccgacctc gaggtgaatg gcgttgaccg
      241 agtagtcgcg gaatagtcgc gaagtacgtc gtggcagccg gacgaggctg tcgtcccctg
      301 gacgagcgag ggctgtgtcg tcaacgagct cgggccctcc cggactgagc ggcaccaaca
      361 cgctgcgact tccggcgcct tgaacccctc ggtccctcac gggacgtgtg ggttcgaaac
      421 ccaccccggg caccgatttc tcgggctttt cgctccggcg agcggctcga accgcgccca
      481 gatcggcttc gaattccaat cgccacccat tgactcagta ttggttgagt tactatggtc
      541 catcgcgtga cggaccttgc atccatcgaa gctcgtgccg cccggcacgc ggccctcggc
      601 gatccgatcc gcctggcgat cgtcgacgaa ctggcggtgt cggatcgggc accgatcgag
      661 ctccgtggct tggtcggcct cgagtcgaat ctcctcgcac atcacctcga cgtgttggaa
      721 gaggtgggac tgatcagtcg gtcgcgatcg agtggcgacg gtcggcgccg atacgtgcat
      781 ctgatccgcg ccgcgctcga tgggctgacg ccggggcggc acatcccgat cggcccggcg
      841 ttgttcatct gtacggcgaa ctcggcgcgc agccaactcg ctgcggcact gtggcgcgaa
      901 ctctcgggcg agtccggcga ctcggccggc acccaccccg tcgaccgggt caaccccgga
      961 gccgtcgccg ccgcgagtcg ggccgggctc gacgtggcag gcgcaacccc acgcgaactc
     1021 accgacgggg aacgacttcc cggcctcgtg gtgaccgtct gtgaccgggc acacgaggaa
     1081 ctcgccggcg acgagacctg gttgcactgg tcgatccccg acccggtccc gctcggcacc
     1141 aaggcggcct tcgacgccac cgtcgccgag cttcgggacc ggatcgagtc gatcctcgac
     1201 gagcgagacc tcgcatcgtg atcgatccgc tgcacccctc cgacgccgaa ctctccgtcc
     1261 agcccgagac cggtaccggc caactccgcc tcgattttgc ccgacgactc acggccgaag
     1321 cgctcggcac cgcactcctg atcatcgccg tcatcggctc gggcatcatg gcgagccgcc
     1381 tctcccccga cgacatcggt ctccaactcc tggagaacgc cgccgccacg gccggagcgc
     1441 tcatcggtct gatcctcatc ttcggagcgg tctccggtgc ccacttcaac ccggtcgtca
     1501 cgctcgtcga ccggctcctc ggcaccgtga ccacccgaga cgccggcctg tacgtcgtcg
     1561 cccagaccat cggcgggtgc gtcggaacgg tcctggcgaa cctcatgttc gaactgcccg
     1621 ccgtcgaact gtccaccacg accaggtcct ccggcgcgtt gtggttgtcg gaggccatcg
     1681 cgacggtcgg gctgctgctc gtcatccacg gctgcgtccg aaccggcaag gcgtccgccg
     1741 tcccgttcgc cgtcgggctc tggatcggcg gggcgtactg gttcacgtcg tcgaccagct
     1801 tcgccaaccc cgccgtcacg atcgcccgca cgctgtcgga caccttcgcc ggaatccgcc
     1861 cgtcgtcggc accgatgttc atcgtcatgc aagtcatcgg cgccggcatc gcctatgcgc
     1921 tcatccgatt cctctatccc cacaacctct cggagacccc ccatgagtga gcaccgtccc
     1981 gaagtcctgt tcgtctgcat ccacaacgcc gggcgctcgc agatggctgc ggcgctgctc
     2041 gctcaccacg gcggcgacca ggtggtcgtc cgatcggcgg gcaccgctcc ggccgagacg
     2101 gtcaaccccg ccgtggtcga agccatggcc gagctcggca tcgacctgca cgcgtccgga
     2161 gcgttcccca agcgcctcga cgacgccgcg gtcgaggcgt cggacgtcgt catcacgatg
     2221 gggtgcggcg acgagtgccc gttctacccc ggcaagcgct acctcgactg ggcgctcgac
     2281 gacccggccg ggcaaggcgt cgacgccgtc cgtccgatcc gcgacgagat cgaccgccgg
     2341 atccgtgagc tcctcgccga gctcctcccc tccgcaactc cctgaactcg aacggaaccc
     2401 tcgaatggaa tcctcacgcc tcgacacagg tcgcgcccgc gtcgtcttcg ccggacagct
     2461 cgacaccgat cccgccggat atctcgtcac cgaaccgggc tcgagccgaa ccaacgtcgc
     2521 cggagtg
//
LOCUS       DAOSOA010000403         2515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_41374_length_2515_cov_6.286585, whole genome shotgun sequence.
ACCESSION   DAOSOA010000403 DAOSOA010000000
VERSION     DAOSOA010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41374_length_2515_cov_6.286585"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PKY13_16395"
     CDS             <1..749
                     /locus_tag="PKY13_16395"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HOT81575.1"
                     /translation="IQRQVRAIASILEDHSRIPAIAAELALIHDLQTDEWWTDVTLQM
                     IETTRRRLRPLVPLIEKRRRAPLYTNFSDDIGEGVEVDLGIGGTDDFAQFRSKATAFM
                     REHNDHLAIQRLRRNQPITESDLTELARMLFEVRGGSEVNVQRASVETGGLGLFVRSL
                     VGMDRSAAKDAFAGFLDERVYSGNQIEYVNLVIDHLANDGVVEPRRFYESPYTDVAPA
                     GPDGVFSRADVERFIGVVHEIRATATAETG"
     gene            860..1213
                     /locus_tag="PKY13_16400"
     CDS             860..1213
                     /locus_tag="PKY13_16400"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOT81576.1"
                     /translation="MKYFVLLVDGGEEPAWDDLTAEEQEAALAAHGAFGEACEARPGV
                     EIVGGEALGDGSTATTLRTRGGEITITDGPYAEAAEHIGGFYVLDAPDLDTVIELCRI
                     LPAYDIDIRPVPDLG"
     gene            1176..2435
                     /locus_tag="PKY13_16405"
     CDS             1176..2435
                     /locus_tag="PKY13_16405"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOT81577.1"
                     /translation="MTSTSDRSPTSADGVIGQLYRAEWGRLVALLVSRSRRLDLAEDA
                     VSEAFAHASSRWPADGVPANPVGWLYTTAHRHVLGRVRTEQVAGRRAPLLAVRPEWVA
                     PDEADDALPDERLHLILLCCHPALPRDSRSALALRLVIGTSTAEIARLFLVPVPTMAA
                     RLTRAKKKIVAAGIPLGAPVADELVGRLDEVCRTIYLAFTAGYTPGDGPQLLRSDLAD
                     DAVQLASVLHAVVPGAPQGRALLALLLLQHARRNTRERDGRLVTLADQDRSSWNADEI
                     DRGLALLDALPPSDGYAEDLRLQALIAAEHARAPTSSATDWAVVADLYAALERRTGSP
                     VVRLNRAVAVAEADGPLAGLAVLDGLEDVLGHHHRLAAVRSELAHRSGDTELARTSYR
                     RAIGQCQNDVERAYLIGRLALLDQPGT"
BASE COUNT          389 a          928 c          816 g          382 t
ORIGIN      
        1 ggatccaacg acaagtacgg gcgatcgcct cgatcctcga ggatcacagc cgcatcccgg
       61 caatcgccgc cgagttggcc ctgatccacg atctccaaac cgacgagtgg tggaccgacg
      121 tcacgctgca gatgatcgag acgactcgcc ggcgcctccg ccccctcgtg ccgctgatcg
      181 agaagcgccg ccgtgccccg ctctacacga acttcagcga cgacatcggc gaaggcgtcg
      241 aggtcgacct cggaatcggg ggaaccgacg acttcgccca gttccgatcg aaggcgaccg
      301 cgttcatgcg ggagcacaac gaccacctgg ccatccaacg ccttcggcgc aaccagccga
      361 tcaccgaatc agacctcacc gaactggctc gaatgctgtt cgaggtacgc ggaggctcgg
      421 aggtcaacgt gcaacgagcc tcggtcgaga ccggtggtct cgggctgttt gtcagatcgc
      481 tggtcggcat ggaccgatcc gcagccaagg acgcgttcgc cgggttcctc gacgagcggg
      541 tctactcggg caaccagatc gagtacgtga acctggtgat cgaccatctc gccaacgacg
      601 gcgtggtcga gccacgtcgc ttctacgagt cgccgtacac cgacgtggcg ccggccgggc
      661 ccgacggcgt cttcagtcgg gcggacgttg aacgcttcat cggcgtggtc cacgagatcc
      721 gagcgacggc gacggccgaa accggctgac gcccggaact ccgccgccga tttttcggcg
      781 acgagtgtcg atttcgccgc acccggcgcg tcagagtggt gtcggtgatc gtcaccggcc
      841 gtgaggtgaa ggagttgcca tgaagtactt cgttctgctg gtcgacggcg gcgaggagcc
      901 ggcctgggac gacctgaccg ccgaggagca agaggccgcg ctggccgccc acggcgcgtt
      961 cggcgaggcc tgtgaggctc ggcccggtgt cgagatcgtc ggcggggagg cgctgggtga
     1021 cggctcgacg gccacgacac tgcgcactcg cggcggcgag atcacgatca ccgatggtcc
     1081 ctacgccgag gcggcagaac acatcggcgg gttctacgtg ctcgatgccc ccgacctcga
     1141 caccgtcatc gagctctgcc ggatcctgcc cgcgtatgac atcgacatcc gaccggtccc
     1201 cgacctcggc tgacggcgtc atcgggcagc tctaccgggc cgagtggggc cggctggtgg
     1261 cgctgctcgt gtctcgctcc cgacgcctgg atctggccga ggacgcagtc agcgaagcgt
     1321 tcgcacacgc atcgtcgcga tggccggccg acggggttcc ggcgaatccc gtcggctggt
     1381 tgtacacaac ggcgcaccgg cacgtgctcg gtcgcgtccg aaccgagcag gtggcgggcc
     1441 gccgagctcc gctcctcgcc gttcggccgg agtgggtcgc gcccgacgaa gccgacgacg
     1501 cgctgcccga cgaacgcctg cacctcatcc tgttgtgctg tcacccagcg ctccctcgcg
     1561 actcacggtc cgcgctcgcc ctccgactcg tcatcggaac gtccacggcc gagatcgccc
     1621 ggctcttcct cgtgccggtc cccaccatgg cggcgcgcct cacccgcgcc aagaagaaga
     1681 tcgtcgccgc cggcatcccc ctcggcgccc cggtcgccga cgagctcgtt gggcggctcg
     1741 acgaagtctg caggacgatc tacctcgcgt tcaccgccgg gtacacgcca ggcgacggcc
     1801 ctcagctgct gcgctccgac ctcgccgacg atgcagtcca gctcgcctcc gtgctccacg
     1861 ccgtggtgcc cggcgcgccc cagggacgag cactgctcgc gttgctccta ctgcaacacg
     1921 cccggcggaa cacccgggag cgggacggcc gcctcgtgac cctcgccgat caggatcgct
     1981 cgtcgtggaa tgccgacgag atcgaccggg gtctcgccct cctcgacgcc cttccaccga
     2041 gcgacggata cgccgaggac ctccgcctgc aggcgctcat cgccgccgag catgcccggg
     2101 ccccgacctc gtctgcgacc gactgggccg tcgtcgccga cctctacgcc gcgctcgaac
     2161 gacgcacggg ctcgccggtc gtgcgcctca atcgcgccgt ggccgtcgcc gaggccgacg
     2221 gcccgctcgc aggcctcgcc gtgctcgatg ggctggagga cgtgctgggt caccaccacc
     2281 gcctcgctgc ggttcgctcc gaactcgccc atcgctccgg cgatactgaa ctcgcccgaa
     2341 cgtcgtaccg ccgtgcgatc gggcagtgcc agaacgacgt ggagcgtgcc tacctgatcg
     2401 gacgcctcgc gctgctcgac caacccggca cgtaggccac actgctcagc cgatcgcgct
     2461 ggccgactac ccgatggcgc cggtctcgcg cagcgcggcg atcttggcct cgtcg
//
LOCUS       DAOSOA010000404         2500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_41711_length_2500_cov_5.389366, whole genome shotgun sequence.
ACCESSION   DAOSOA010000404 DAOSOA010000000
VERSION     DAOSOA010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41711_length_2500_cov_5.389366"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKY13_16410"
     CDS             complement(<1..669)
                     /locus_tag="PKY13_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOT81578.1"
                     /translation="MSPGASTEALLAWGEQVRRDLPWRATRDPWAVLVSEVMLQQTQV
                     ARVVPKWTEFLRRWPTPVAAAAASFSEILEVWTGLGYPRRARALHDTAKVVTAEFDGR
                     FPEVLDALLGLPGIGPYTARAVLAFAFEQDVGVVDTNIARVLARRAGRRLTPREAQEL
                     ADDFVAPGTGWAHNQSLMDLGAMRCRPTPDCDGCPLRPTCRWAAAGWPAPDPAVGSAG
                     VSGRQ"
     gene            complement(647..1435)
                     /locus_tag="PKY13_16415"
     CDS             complement(647..1435)
                     /locus_tag="PKY13_16415"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT81579.1"
                     /translation="MSTPDRVHEAAAVGFGREAASYAGIRPSYPQAVVDLVAHDTPAG
                     GTVCDLAAGTGIFTRQLLRHGLDVTAVEPVEGMRRQFHVDTPAGHVVDGTAEDLPFHD
                     ATFDVVTVAQAFHWFDAPVALAEIRRVLKPLGRLFLIWNERDHSEAWIRDWTQVVDSL
                     ALGEGVRPYHRHQDTDWAAVVDGGGGFTPVEHSVYDNPTVGTPDGLVERARSMSYVAS
                     LDDERQRAVLDAVRQLVATHPDLAGSATFPFPYRTHVYVARRLD"
     gene            complement(1532..2041)
                     /locus_tag="PKY13_16420"
     CDS             complement(1532..2041)
                     /locus_tag="PKY13_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOT81580.1"
                     /translation="MTTHDRPFGRHLEDFEVGDIYKHWPGKTITEYDDHLFCMITMNH
                     HPLHTNAWFAESSVQGRNVVVGNLVYSLILGMSVPDVSGAAIANLAVETLQHKNPTFH
                     GDTIYAETRVLEKRESSKGDRGIVTVETKGFNQDGLEVCYFKRKVMVWKRDSAPGRVR
                     PYDDAGWSE"
BASE COUNT          398 a          833 c          897 g          372 t
ORIGIN      
        1 ctgccgaccg ctgacgccgg ccgagccgac ggcggggtcg ggcgcgggcc atcccgccgc
       61 cgcccaccgg cacgtgggtc gcaacgggca cccgtcgcag tccggtgtcg gtcggcaccg
      121 catggcgccg aggtccatga gcgactggtt gtgcgcccat cccgtgccgg gggccacgaa
      181 gtcgtccgcg agctcctgcg cttcgcgggg tgtgaggcgg cggcccgccc gacgagccag
      241 gactcgggcg atgttcgtgt cgacgacgcc gacgtcctgc tcgaacgcga acgccagcac
      301 ggcgcgggcc gtgtacgggc cgatgccggg gaggccgagg agcgcatcga ggacctcggg
      361 gaatcgaccg tcgaactcgg cggtcacgac cttggcggtg tcatgcagcg cccgggcccg
      421 acggggatac ccgagaccgg tccacacctc caggatctcg gagaacgagg ccgccgccgc
      481 ggccaccggc gtcggccagc ggcgcaggaa ctccgtccac ttcgggacca cccgcgccac
      541 ctgggtctgc tgcagcatca cctcgctgac gagcaccgcc cacgggtcac gggtcgcccg
      601 ccacggaagg tctcgtcgta cctgctcgcc ccaggcgagc aacgcctcag tcgaggcgcc
      661 gggcgacata cacgtgggtg cggtacggga acgggaacgt cgccgagccg gcgaggtcgg
      721 gatgggtggc gacgagttgg cgcaccgcgt cgagcacggc acgctgccgt tcgtcgtcga
      781 gcgacgcgac gtaactcatc gagcgggccc gttcgacgag cccgtcgggt gtgcccacgg
      841 tcgggttgtc gtagaccgag tgctcgaccg gtgtgaatcc gcctccgccg tcgacgacgg
      901 ctgcccagtc ggtgtcctga tggcggtggt agggccgcac cccctcaccg agggccagcg
      961 aatcgacgac ctgcgtccag tcccggatcc acgcctcgga gtgatcgcgt tcgttccaga
     1021 tcaggaagag tcggccgagc ggcttgagca cccgccggat ctcggccagc gcgaccggtg
     1081 catcgaacca gtggaacgcc tgggcgacgg tcaccacatc gaacgtcgcg tcgtggaacg
     1141 gaaggtcctc ggccgtcccg tcgaccacat ggccggccgg tgtgtcgacg tggaactggc
     1201 gacgcatgcc ctcgaccggc tcgacggcgg tcacgtcgag cccgtggcgc aacagctgtc
     1261 gggtgaagat gcccgtaccg gctgccagat cgcagacggt gccacccgcg ggggtgtcgt
     1321 gcgcgacgag gtcgacgacc gcctgggggt acgacggacg gatgccggcg tacgacgctg
     1381 cctcccgacc gaatccgaca gcggctgctt cgtggacccg atcgggagtg ctcatcgtct
     1441 cagccctcgg cctcggcgtc ggtccagccg aggtaccgca gcgtaagaag cgagcgcagc
     1501 gagcgaacct gagcgaaggt ggccgaggcc atcattcgga ccatccggcg tcgtcgtagg
     1561 ggcgcacccg gcccggtgcc gagtcgcgct tccagaccat gaccttgcgc ttgaagtagc
     1621 agacctcgag gccgtcctgg ttgaagccct tggtctcgac ggtgacgatg ccgcggtcgc
     1681 ccttggacga ctcgcgcttc tcgagcaccc gggtctcggc gtagatcgtg tcgccgtgga
     1741 acgtcgggtt cttgtgctgg agcgtctcga ccgcgaggtt ggcaatggcc gcaccggaaa
     1801 cgtcgggcac gctcatgccg aggatcagcg agtagacgag gttgccgacg accacgttgc
     1861 gaccctggac cgagctctcg gcgaaccagg cgttggtgtg caacgggtgg tggttcatcg
     1921 tgatcatgca gaacagatga tcgtcgtact cggtgatcgt cttgccgggc cagtgcttgt
     1981 agatgtcgcc gacctcgaaa tcctcgaggt ggcggccgaa gggtcgatcg tgcgtagtca
     2041 cggccgccaa cggtagccgc cggtaccgca gcgtaagaag ttgccgaggg acgaggcaac
     2101 gaacctgagc gaaggtggcc ggcgacatca ggacgccgcc ggtaccgcag cgtaagaagt
     2161 tgccgaggga cgaggcaacg aacctggcgg aggtggccac cgagaaaggt tcagcgagac
     2221 ccccgagcgg ccgaaagatg ttccatcaga acggttctca gcgctccggc tccgcgcccc
     2281 gacgaggtcg acggtgtcgt cgcgcccgaa gacgggcggg tcggtgcgct gcgccggggg
     2341 atcgcccgcc ggatcaccga gaccaccact gcctccgagc tgccgttcgc ctactggcgg
     2401 cgaatgctga tcggccgttg ggtgctcggc gtgacgttgt tcgggatcat cgcgatcgct
     2461 ccgatgttcg gcccgcaccg cctgtggttc gcgacggtgt
//
LOCUS       DAOSOA010000405         2493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_41873_length_2493_cov_6.484824, whole genome shotgun sequence.
ACCESSION   DAOSOA010000405 DAOSOA010000000
VERSION     DAOSOA010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41873_length_2493_cov_6.484824"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PKY13_16425"
     CDS             complement(<1..1061)
                     /locus_tag="PKY13_16425"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT81581.1"
                     /translation="MTTPANLTALVGGRVIDPANGFDGIADVVCGDGKVLAVGSGVAA
                     TYLDADRIDCTNLIVTPGLIDLHVHVYPGLGNFCVTPDQAGVERGVPIVVDGGTSGTA
                     TMQLALDWLDAAQPKTKVLSFMDPCVLYLATHDFICHKLEIANDTRNLDLDAAADLLE
                     LHADRIVGFKVRACHTGDPEVSPFLEGAKSIAGDRPIMVHLGRFPHTASITTDALLGS
                     LREGDIVTHAFRGASGALTIPERLPSDGLRKAADRGVLLDVGHSATDFRFRDARTIIE
                     NGYRPDTISTDMNRFNIDHPVGSLAETMSKILALGVPLVDVVAMVTTNPAAAIHRSGE
                     YGTLDVGRSAEVSVMRMEAG"
     gene            complement(1058..>2493)
                     /locus_tag="PKY13_16430"
     CDS             complement(1058..>2493)
                     /locus_tag="PKY13_16430"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT81582.1"
                     /translation="DDDGALRGIRVAADRDCGAYFSIFGAGPLFAMTGTIGGPYTWDA
                     VTAHARAFATNTCPTHSYRGFGQAQATFVRELAVDAVAADLGMDPFDLRLRNCITPDQ
                     QPFPLRTGPMFYDDGDYPESLRRARALAAAWPDPPDDGRRWGTACSINIQQAGVGPSQ
                     GNPHVGLSIGSWESTTIRMERDGTVRVYVGVSSHGQGHATTFAQLVADRLGIDPSQVE
                     IIHSDTDVTPYSAYGTAASRSIAVGGGATVRAAEQLASRLVSLAALLLETDPTNVMFL
                     DGAAVLRDDPSRRLPLAAVADRAWQGWGIPEGYEAGLVEHAVYEPPEFTWSYSTHVCR
                     LAVDTTTGQIEIDRYAMVLDCGTVVNPTIVEGQLHGGMVQGLGPALCEEVLVDDAGVP
                     LTTTLLDYLIPSSAIVPDMQVELMEIPSPHTPGGMKGMGEGGTNGAFSCIANAVRAAL
                     PEHADRLTRTPFTPSRVWDAVHPEGTP"
BASE COUNT          374 a          812 c          923 g          384 t
ORIGIN      
        1 ccggcctcca tgcgcatcac cgacacctcg gcgctgcggc cgacgtcgag cgtgccgtac
       61 tcgccgctgc ggtggatggc cgccgccggg ttcgtggtca ccatggcaac gacgtcgacg
      121 agcggcacgc cgagcgccag gatcttcgac atcgtctccg ccagcgaccc caccgggtgg
      181 tcgatgttga accggttcat gtcggtcgag atcgtgtcgg gccggtaccc gttctcgatg
      241 atcgtgcgag cgtcgcggaa ccggaagtcg gtcgcggagt gtccgacgtc gagcaggacg
      301 cctcggtcgg cagccttccg caggccgtcc gatggcaggc gctcggggat cgtcagcgct
      361 ccgctggcac cccggaaggc gtgggtcacg atgtcgccct cccgcagcga gccgagcagg
      421 gcgtcggtcg tgatcgacgc ggtgtggggg aaccgtccga ggtgcaccat gatcggccgg
      481 tcaccggcga tcgacttggc accctcgagg aacggagaca cctcggggtc gccggtgtgg
      541 caggcccgca ccttgaaccc gacgatgcgg tcggcgtgca gctcgagcag gtccgcggcg
      601 gcatcgaggt cgagattgcg ggtgtcgttg gcgatctcga gcttgtggca gatgaagtcg
      661 tgagtcgcca gatacagcac acacggatcc atgaagctga ggaccttcgt cttcggctgg
      721 gcggcgtcga gccagtcgag ggcgagctgc atcgtggccg tgcccgatgt gccgccgtcg
      781 acgacgatgg gcacgcctcg ttccacgccg gcctgatcgg gggtcacgca gaagttgccg
      841 agccccgggt acacgtgcac gtggaggtcg atgaggcccg gggtgacgat caggttggtg
      901 cagtcgatgc ggtcggcgtc gaggtaggtg gctgcgacgc ccgacccgac ggccagcacc
      961 ttcccgtcgc cacagacgac gtcggcgatc ccgtcgaagc cgttggccgg atcgatgacc
     1021 cggcctccga cgagggcggt gaggttggct ggggtggtca tggggttcct tctggatgga
     1081 cggcgtccca gacgcgggac ggggtgaacg gagtgcgggt gagtcggtcg gcgtgctcgg
     1141 ggagcgcagc tcgaacggcg ttggcgatgc agctgaacgc gccgttggtg ccgccctcgc
     1201 ccatgccctt catgccaccg ggggtgtgcg gcgaggggat ctccatcagc tcgacctgca
     1261 tgtccgggac gatcgcggag ctggggatca ggtagtcgag cagtgtcgtg gtgagtggaa
     1321 cgccggcgtc gtcgaccaac acctcttcgc acagcgccgg accgagtccc tgcaccatgc
     1381 cgccgtgcag ctgcccctcg acgatggtcg ggttcacgac ggtgccgcag tcgaggacca
     1441 tcgcgtaacg gtcgatctcg atctgccccg tcgtcgtgtc gacggcgagc cggcacacgt
     1501 gggtcgagta cgaccacgtg aactccggtg gttcgtacac ggcgtgctcg accagccccg
     1561 cctcgtagcc ttccgggata ccccagccct gccaggcgcg atcggcgacg gcagccaggg
     1621 ggagccgccg cgacgggtcg tcgcgcaaca cggcggcacc gtcgaggaac atcacgttgg
     1681 tcggatcggt ctcgagcagg agcgcggcca gcgacacgag tcggctcgcc agctgctcgg
     1741 cggctcgcac ggtggccccg ccgccgacgg cgatcgatcg cgacgccgcg gtgccgtacg
     1801 ccgagtacgg ggtgacgtcg gtgtcggagt ggatgatctc gacctgggac gggtcgatcc
     1861 cgagccggtc ggcgacgagc tgggcgaacg tcgtggcgtg gccctggccg tgcgaggaga
     1921 ccccgacata gacgcggacg gtgccgtccc gctccatgcg gatcgtcgtc gactcccacg
     1981 acccgatgct caggccgacg tggggatttc cttgcgacgg accgacgccg gcttgctgga
     2041 tgttgatcga gcatgcggtt ccccaccgcc gaccgtcgtc gggcggatcg ggccacgccg
     2101 ccgcgagcgc tcgggcccgc cgcaacgatt ccgggtagtc gccgtcgtcg tagaacatcg
     2161 ggccggtgcg caacgggaac ggctgctgat cgggggtgat gcaattgcgg agccggaggt
     2221 cgaacgggtc catgcccaag tcggcggcca ccgcgtcgac cgcgagctca cgcacgaacg
     2281 tcgcctgcgc ctggccgaaa ccccggtagg agtgggtggg gcacgtgttg gtcgcgaagg
     2341 cccgggcgtg ggccgtgacg gcgtcccagg tgtagggccc gccgatcgtg cccgtcatcg
     2401 cgaacagcgg accagcaccg aagatcgaga agtacgcgcc gcagtcacga tcggcggcca
     2461 cacggatgcc ccgcagcgcg ccgtcgtcgt cgt
//
LOCUS       DAOSOA010000406         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_42512_length_2466_cov_3.374119, whole genome shotgun sequence.
ACCESSION   DAOSOA010000406 DAOSOA010000000
VERSION     DAOSOA010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42512_length_2466_cov_3.374119"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKY13_16435"
     CDS             complement(<1..371)
                     /locus_tag="PKY13_16435"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT81583.1"
                     /translation="MRGQSPLLLSPNAPDALVASSPPLPKEVRANVSSDVAALLLTLG
                     GVSLVVGALGIANVTLVSVLERTGEIGVRRALGALRRHVALQFLGESTVLGFLGGAVG
                     SSVGVVIVVAVSAAKSWSPVLP"
     gene            complement(376..1101)
                     /locus_tag="PKY13_16440"
     CDS             complement(376..1101)
                     /locus_tag="PKY13_16440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT81584.1"
                     /translation="MGDRSDAPPAIRRPRFSLRDAVWEASAGVLARPARSALTVVGTV
                     LGIAALVATVGLASTAGAQILTRLDVLAATDVVVEPRAGRASVLGWDAADEVALLNGV
                     RSAALISPVVDDEGSGPDVATVLASDDRTDVIAVSAGVWEVTDATLGRGRFLGPVDES
                     ATLPTAVLGATAAARLGIFDVSAAPTVFVDGHPVTGRRDPRGNSSAPGALGSGDGDHC
                     VRPGRTRPAGPESSADRDRPRRE"
     gene            complement(1094..1786)
                     /locus_tag="PKY13_16445"
     CDS             complement(1094..1786)
                     /locus_tag="PKY13_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT81585.1"
                     /translation="MTWPSASIARPIVEMRGVRRVFGTEPEVVALDEVDLTVRRGEFV
                     AIVGRSGSGKSTLLNTLGLMDTPSAGAYHLDGHDTAALSAAHRAGLRGARLGFVFQAF
                     HLVPSRSVTENVTMAGMYAGATRSSRRSAAIEVLERVGLGHRLDAAPSALSGGERQRV
                     AIARALASRPALLLADEPTGNLDSATSDDILGLFAELHADGVTIVMITHDEGVAALAQ
                     RTLRMTDGVLRG"
     gene            complement(1783..>2466)
                     /locus_tag="PKY13_16450"
     CDS             complement(1783..>2466)
                     /locus_tag="PKY13_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81586.1"
                     /translation="AEARLADQAAIVGAGVGVTEVAFFPSLPRRVRSVEGEVGGDAAG
                     ELLRVTGGDAVGRWFVSADEREQLQVGGSVQLSDATIGLQVDGTIESIASTPGDPDAV
                     DSDAAGSTPAAAGSGTGGGESSSRANRFLVTIAIVRGDRTPPDDELVGRDLLVSAPVS
                     ESHRGLAVPVAALTSRSDGGAVVRRSTGRFDAVDVRVTIGATGNGYASVVPDIAGTLA
                     VDDRVVVGR"
BASE COUNT          410 a          934 c          824 g          298 t
ORIGIN      
        1 ggcaggaccg ggctccacga cttggccgcc gacacggcca cgacgatcac cacccccacc
       61 gacgaaccca ccgctccgcc caagaagccc agaaccgtcg actctccgag gaactgcagg
      121 gcgacatggc gccgcagggc acccagcgcc cggcgtaccc cgatctcacc ggtgcgttcg
      181 agcaccgaca ccaacgtcac gttggcgata cccagcgctc ccacgaccaa cgacaccccg
      241 ccgagggtga gcagcagcgc ggccacatcg gagctgacgt tggcgcggac ctctttcggt
      301 agcggcggcg acgacgccac caacgcgtcg ggagcgttcg gggacagcag cagtggagac
      361 tgaccgcgca ccagctcatt cgcgccgagg acggtctcga tctgcactcg actcggggcc
      421 cgcaggccga gttcggcccg ggcgaacgca gtggtcacca tcaccgctcc caagagctcc
      481 gggcgccgac gagttgcccc gaggatcccg acgacccgtc accggatgac cgtcgacgaa
      541 gacggtgggg gcggccgaga catcgaagat gccgaggcgc gccgccgcag tagcgccgag
      601 caccgcagtc ggaagggtcg ctgattcgtc gaccggaccg aggaaccggc cgcgccccaa
      661 cgtggcatcg gtgacttccc acacgccagc ggacacggca atgacatcgg tgcggtcgtc
      721 ggacgcgagc actgtcgcga catcgggtcc actgccctcg tcgtcaacga cgggcgagat
      781 gagcgcggcc gaccgcacgc cgttgagcaa cgccacctcg tccgcagcgt cccaacccag
      841 cacgctggcg cgtcccgccc ggggctcgac caccacgtcg gtcgcggcca ggacgtcgag
      901 gcgggtcagg atctgcgcgc cggccgtcga cgccaagccg accgtggcga ccagtgccgc
      961 gatccccagc accgtcccga caacggtcaa cgccgatcgt gccggccggg cgagtacacc
     1021 ggcggatgcc tcccacacgg cgtcgcggag actgaagcgg ggccggcgga tggccggcgg
     1081 ggcgtcggag cggtcaccca cgcagcactc cgtcggtcat ccggagtgtg cgctgggcca
     1141 gcgcagcgac cccctcgtca tgggtgatca tcacgatcgt gacgccgtcc gcgtgcagtt
     1201 cggcgaacaa cccgaggatg tcgtcggacg tcgcggagtc gaggttgccc gtcggctcgt
     1261 cggcgagcaa cagagctggc cgcgaggcga gcgcccgagc gatggcgacg cgttggcgtt
     1321 cgccacccga caacgccgac ggcgcggcat cgagccgatg gccgagcccg acccgctcga
     1381 ggacctcgat cgcggcggaa cggcgactcg aacgagtcgc gcccgcgtac atccccgcca
     1441 tcgtgacgtt ctccgtcacc gaccgcgagg gcacgaggtg gaaggcctgg aacacgaagc
     1501 cgagtcgagc gccgcggagc ccggcgcggt gcgccgccga cagcgctgcc gtgtcgtggc
     1561 cgtcgaggtg gtaggcgccc gcgctcgggg tgtccatcag ccccagtgtg ttgagcagtg
     1621 tcgacttgcc agagccggac cgacccacga tggccacgaa ctccccccgc ctcacggtca
     1681 ggtcgacctc gtcgagcgcc acgacctcgg gttccgttcc gaacactcgc cggacgcccc
     1741 gcatctccac gatcggtcgg gcgatcgatg cgctcggcca cgtcaccggc cgaccaccac
     1801 tcggtcgtcg accgccagcg tgccggcgat atcgggtacg acgctggcgt acccgttgcc
     1861 ggtggcaccg atggtgacgc gtacgtcgac ggcgtcgaat cgtccggtcg accttcgcac
     1921 caccgcgccg ccgtcggatc gtgacgtcag ggcagccacc gggaccgcca gccctcgatg
     1981 tgactccgac accggcgccg acacgagcag gtcgcgtccg accagctcgt cgtcgggcgg
     2041 tgtgcgatcc ccccgaacga tcgcgatcgt cacgaggaac cggttcgccc ggctggacga
     2101 ctcacctcca ccggtgccgc tccccgccgc tgccggggtc gacccggcag cgtcactgtc
     2161 gacggcgtcg ggatcaccgg gcgtcgaggc gatcgactcg atggtgccgt ccacctgcag
     2221 cccgatcgtg gcgtccgaca gctggaccga cccgccgacc tgcaactgct ctcgttcgtc
     2281 ggccgacacg aaccagcgcc cgacggcatc cccgccggtc acacggagca actcgccggc
     2341 agcgtcgcca ccgacctcgc cctcgacgct gcgcacccgc cgcggcaacg acgggaagaa
     2401 ggccacctcg gtgacgccaa caccggcgcc gacgatcgcc gcctgatcgg cgaggcgggc
     2461 ttcggc
//
LOCUS       DAOSOA010000407         2464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_42554_length_2464_cov_3.893317, whole genome shotgun sequence.
ACCESSION   DAOSOA010000407 DAOSOA010000000
VERSION     DAOSOA010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42554_length_2464_cov_3.893317"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKY13_16455"
     CDS             complement(<1..346)
                     /locus_tag="PKY13_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81587.1"
                     /translation="MTSSPGHSGPTPSPHRWWAAIGSAVLAGLVVGVLVAGLAPWSQD
                     ASADADRASIRTQVAGISIERSSVDDEVESTVVSTLPAPTTTAAPTITAAPVTAPPTT
                     AAPTTAVPTTAPA"
     gene            491..1537
                     /locus_tag="PKY13_16460"
     CDS             491..1537
                     /locus_tag="PKY13_16460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT81588.1"
                     /translation="MPASSALSLVDRPRTAFGQTLRRRAVTVPAAIAGLVIVAPLLAA
                     SLPLLAALDLIHGEPILPRTRLAVFGVWYLAWEAVAVVAAAVLWVATGFGRGVDRAWS
                     QSAHAALQSTWVDSILSVARRMLRLDLDIEGADALGTAPTDGPLIVLCRHASMVDTLV
                     PAKLLFDRGYRVRYVLKDELLWDPALDIIGHRLPNCFVDRSSTDKAAELDAIGALAAG
                     AGPGEALVIFPEGTRWSPEKRHRAIERLRTDDPAAAERAERLTSTLLPRPAGTLALLA
                     GRPDADVVIVSHTGLEGLAGPKDALRLLPLRRPMQVSFRRVRRGEVPDSPDAQRDWLL
                     DQWTQVDDWIRAAR"
     gene            1579..>2464
                     /locus_tag="PKY13_16465"
     CDS             1579..>2464
                     /locus_tag="PKY13_16465"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOT81589.1"
                     /translation="MDFSVWPASNRTWAEVADTAVAADRDGWHGVWFADHFMPNVAGT
                     VDADDPVLEVWSVLAGLAAVTSRLRLGPLVTSVTYRHPAVLAKIASTTDQISGGRVVL
                     GIGAGWQVNEHEAYGLELGDVPTRSDRLEEAAAILRSLLHEPRTTLVSEFFTVVDAPN
                     EPKSAGSPLPLLVAGKGERRTLRTAARHADEWNGWCRPDEMIAKRAVLARHCEAAGRD
                     VADIRCSTQAFVCFTDGSAPPSEFLAATTGRPTLVGTADQIVEQVAAHADAGADELIV
                     PEWLWDDPAERADQLARFG"
BASE COUNT          324 a          907 c          877 g          356 t
ORIGIN      
        1 gggccggggc ggtggtcggc acggcggtcg tcggcgcggc ggtcgtcggc ggcgcagtga
       61 ccggtgccgc cgtgatcgtc ggcgcagcgg tggtggtcgg cgccgggagc gtcgacacca
      121 cggtcgactc gacctcgtcg tcgacgctcg atcgctcgat gctgatgccg gcgacctgcg
      181 tccggatcga cgctcgatcg gcgtcggcgg acgcgtcctg cgaccacggg gccaacccgg
      241 cgacgagcac accgacgacg aggccggcga gcaccgccga accgatcgcc gcccaccaac
      301 gatgtggtga cggtgttggg ccagaatgcc caggggaaga ggtcattcgc gaaccgtacg
      361 tatgaggctg tttgatgtca cttcatatct tctcaatacc tctgaccgcg gttgcccgga
      421 cgcgcaacgt gggtgggcag cccggcaaca tgggtgggca gccctccgac gggggaagta
      481 gaccgtcgtg atgccggcaa gctctgcgtt gtccctggtc gaccgaccgc gcaccgcctt
      541 cgggcagacg ctgcgtcggc gagccgtcac cgtccccgct gcgatcgccg gactcgtgat
      601 cgtcgccccg ctcctcgcgg cgtcgctccc cctcctcgcg gccctcgatc tgatccacgg
      661 cgagccgatc cttccccgca cgcgcctcgc cgtcttcggg gtgtggtacc tcgcctggga
      721 ggcggtcgcc gtggtcgctg ccgccgtact gtgggtcgcg accggctttg gccgcggcgt
      781 cgaccgtgcc tggtcgcaat cggcgcacgc ggccctccag tcgacgtggg tcgactcgat
      841 cctcagcgtc gctcgtcgga tgctccggct cgacctcgac atcgagggcg ccgacgcgct
      901 cgggaccgcc ccgacggacg gccccctgat cgtgctgtgc cggcacgcca gcatggtcga
      961 cacgctggtg ccggcgaagc tgctgttcga ccgtggctac cgggtgcggt acgtcctgaa
     1021 ggacgagctg ttgtgggacc cggcgctcga catcatcgga caccggctgc cgaactgctt
     1081 cgtcgaccgg tcgagcaccg acaaggcggc cgaactcgac gcgatcgggg cgctcgccgc
     1141 cggcgccggg ccgggcgagg cgctggtcat cttccccgag ggcacccggt ggtcacccga
     1201 gaagcggcac cgggcgatcg agcggctccg gacggacgat cccgcggcgg ccgaacgcgc
     1261 cgaacggctg acgtcgacgc tgctcccccg gccggccggc accctggcgc tcctggccgg
     1321 gcgccccgac gccgacgtgg tgatcgtcag ccacaccggc ctcgagggcc tcgccggacc
     1381 gaaggacgcg ctgcgcctgc tgccgttgcg ccggccgatg caggtgtcgt tccgccgggt
     1441 acggcgtggc gaggtccccg actcccccga cgcccaacgc gactggctcc tcgaccagtg
     1501 gacccaggtc gacgactgga tccgggccgc ccgctgaccc cgaccccctc ccatttccga
     1561 ggcgggtagg ttcggttcat ggacttcagc gtgtggccgg cctcgaaccg aacgtgggcc
     1621 gaggtggccg acaccgccgt cgccgccgac cgcgacgggt ggcacggcgt gtggttcgcc
     1681 gaccacttca tgccgaacgt cgccggcacc gtcgacgccg acgaccccgt gctcgaggtg
     1741 tggtcggtgc tcgccggcct ggccgccgtc acgagccggt tgcggctggg cccgctcgtc
     1801 acctccgtga cctaccggca cccggcggtc ctcgccaaga tcgcgtccac caccgaccag
     1861 atcagcggcg gccgggtcgt cctcggcatc ggcgccgggt ggcaggtcaa cgagcacgag
     1921 gcgtacggcc tcgaactcgg cgacgttccc acccggtcgg accggttgga ggaagcggcg
     1981 gcgatcctcc gatccctgct gcacgagccg cgcaccacgc tcgtcagcga gttcttcacc
     2041 gtcgtcgacg cgccgaacga gccgaagtcg gccggttcac cgctcccgct gctcgtcgcg
     2101 ggcaagggtg agcgccgcac gctgcgcacg gcggcccgac acgccgacga gtggaacggc
     2161 tggtgccggc ccgacgagat gatcgccaaa cgagcggtgc tggcccggca ctgcgaggcg
     2221 gccggccgcg acgtcgccga catccgctgt tcgacgcagg cgttcgtgtg cttcaccgac
     2281 ggctcggccc cgccgagcga gttcctggcc gcgacgaccg gccgcccgac gctcgtcggc
     2341 accgccgatc agatcgtcga gcaggtggcg gcgcacgccg acgccggagc ggatgagctg
     2401 atcgtgcccg agtggttgtg ggacgacccg gccgaacgcg ccgatcagct ggcgcggttc
     2461 ggtg
//
LOCUS       DAOSOA010000408         2455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_42777_length_2455_cov_6.207500, whole genome shotgun sequence.
ACCESSION   DAOSOA010000408 DAOSOA010000000
VERSION     DAOSOA010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42777_length_2455_cov_6.207500"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="PKY13_16470"
     CDS             <1..1679
                     /locus_tag="PKY13_16470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOT81590.1"
                     /translation="VNRRPATIDRPSGEPMPDDFWLSTGRGSVLASQFWPTGTVAPES
                     VGVVLVPGIAHEYHSMHFGLVTLARELATVGVPALVIDLAGTAQGSGTLGDPDLAAQW
                     GDDVRCAVDHLRRSGLQHVAVVGTRLGALIAVDALASDPVDLVVLWAPVLSGRRYVRE
                     LQMIEAAASDSAVPDLPGLTIAGYNLPPGLLDQLRGTAVDRIAGKPASRVCLVDVPDR
                     LAAIDLTPTPLAEVPVDRVEAAGTDDWLFSAADGMIAPFADIPTVIAAIRRPFRDASE
                     VTHPPGVTRPDVRGSRRQEFEHDGVAIRETIVRFGRLGLSGILSEPASVDADVGGPAY
                     VAITALGPGRLFPDFARRAAARGRTCLRFDLAGFGTSPRRPGDGWADFYDPEAPAEIA
                     DAIDHVTRRGHDDVVLVAFCAGAWSAVQMAPHPAVRRIVGINLQLFIRHRLLHRRPWP
                     DLPPSKQVLASIGQRRNVRRVVKRVQFSHPLPSPTLRWIRRHRRAGVDVTLVFAEEDR
                     GSGYYRDRVEASRSPFVRRGLPTVRRYEGLGHLPSGATRSRVLDDILSAD"
     gene            complement(1693..1941)
                     /locus_tag="PKY13_16475"
     CDS             complement(1693..1941)
                     /locus_tag="PKY13_16475"
                     /inference="COORDINATES: protein motif:HMM:NF005480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT81591.1"
                     /translation="MSDQTTTIRQVLADHGRLSAAVDSLGDADSLFDAGLSSHAAVNV
                     MLALEDHFDVEFPERFLKRATFESVTSLQGALTEILLP"
     gene            2137..>2455
                     /locus_tag="PKY13_16480"
     CDS             2137..>2455
                     /locus_tag="PKY13_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81592.1"
                     /translation="MLVHNTRRLFDALDLADEAEALGMGIERAEYERLTTLSNWASSF
                     LCRSHEDLGRAGPVCPYARTAIEQGLFLLGEIDLSFDHIDEIAEEIAVYKEWFVSLQG
                     VLSP"
BASE COUNT          324 a          887 c          845 g          399 t
ORIGIN      
        1 gagtgaatcg tcgaccggca acgatcgatc gaccgtcggg cgaaccgatg cccgacgact
       61 tctggctgtc cacgggccgt ggttcggtcc tcgcctcgca gttctggccg accggaaccg
      121 ttgcccccga gtcggtcggc gtggtgctcg tccccggcat cgctcacgag taccactcga
      181 tgcacttcgg tctcgtgacg ctcgcccgcg agctcgcgac cgtcggggtg ccggcgctcg
      241 tgatcgacct cgcgggaacg gcgcagggat ccggcacgct cggcgatccc gacctcgccg
      301 cgcagtgggg tgacgacgtg cgatgcgccg tcgaccatct gcgtcggagc ggcctgcagc
      361 acgtcgccgt cgtgggcacc cgtttgggcg cgctgatcgc ggtcgacgcg ttggcgtcgg
      421 accccgtcga cctcgtggtg ctctgggcgc cggtgctctc gggtcggcgc tacgtccgtg
      481 agctccagat gatcgaggcg gccgcatccg actccgccgt gcccgacctt ccgggcctga
      541 cgatcgccgg gtacaacctg ccgccgggcc tgctcgacca gctgcgcggc acggcggtcg
      601 atcggatcgc cggcaagccg gcgagtcgcg tctgcctcgt cgacgtcccc gatcgcctcg
      661 cagcgatcga tctcaccccg accccgctcg ccgaggtgcc cgtggaccgc gtggaggccg
      721 ccggcaccga cgactggctg ttctccgccg ccgacggcat gatcgcgccg ttcgcggaca
      781 tccccaccgt gatcgctgcg atccgccgac cgttccgcga cgcgagcgag gtcacacacc
      841 cgcccggcgt gacccgcccc gacgtacgcg gctcgcgccg ccaggagttc gaacacgacg
      901 gggtggcgat ccgcgagacg atcgtgcggt tcggccggct cgggctgagc gggatcctca
      961 gcgaaccggc gtcggtcgac gccgacgtcg gtggccccgc gtacgtcgcc atcacggcgc
     1021 tcggtcccgg acgactcttt cccgacttcg cccgccgcgc tgcagctcgc ggccgcacgt
     1081 gcctccggtt cgacctggcc ggcttcggca ccagcccccg ccgacccggc gacgggtggg
     1141 ccgacttcta cgaccccgag gcgcccgccg agatcgccga cgccatcgac cacgtcaccc
     1201 gtcgtgggca cgacgatgtc gtcctcgtcg cgttctgcgc cggggcctgg tctgccgtgc
     1261 agatggcacc gcaccccgcc gttcggcgga tcgtcggcat caacctccag ctgttcatcc
     1321 ggcatcgcct cctgcaccgg cgcccctggc ccgatcttcc gccctccaag caggtgctgg
     1381 cgtcgatcgg ccagcgccgg aacgtccgtc gggtggtcaa gcgggtccag ttctcccatc
     1441 cgctcccgag cccgaccctg cgctggatcc gtcgacaccg gcgggccggc gtcgacgtca
     1501 cgctcgtctt cgccgaggag gatcgcggga gcgggtacta ccgcgaccgc gtcgaggcgt
     1561 cgaggtcgcc cttcgtgcgg cgtggtctgc cgacggttcg acggtacgag gggttgggcc
     1621 accttccgtc gggggccacc cgctcccgcg tgctcgacga catcctgtcc gccgactgag
     1681 cggcgaggaa cgtcagggga gcaggatctc ggtcagagcg ccctggaggc tggtcaccga
     1741 ctcgaatgtc gcccgcttca ggaaccgctc ggggaactcc acgtcgaagt ggtcctcgag
     1801 cgccagcatg acgttgacgg cggcgtggga gctgagcccg gcgtcgaaca ggctgtcggc
     1861 gtccccgagg gagtcgacgg ccgccgagag tcggccgtgg tcggcgagta cctgacgaat
     1921 ggtggtggtc tggtcggaca tggcggagct ttcgggaatg gcccccgatc cgggggtgcg
     1981 atcgggccgg tgaccgcagt gtgcagtggg tcgcccgcag tgtgcacggg gtcggcggga
     2041 ctgtccagcg tggacgatcc cccgttttca ttgccgaact ctgatgtcat atgagacact
     2101 ctcctggctc gtcaccccct ttcgaaaggc acgtcgttgc tggtccacaa cacacgacgc
     2161 ctgttcgacg ccctggatct cgcggacgag gccgaggcgc tcgggatggg catcgagcgc
     2221 gccgagtacg aacgcctgac cacgctgtcg aactgggcgt cgtcgttcct ctgtcggtcc
     2281 cacgaggacc tcggccgggc cgggccggtg tgcccctacg cccgcacggc gatcgaacag
     2341 ggcctgttcc tcctcggaga gatcgatctg tcgttcgacc acatcgacga gatcgccgag
     2401 gagatcgcgg tctacaagga gtggttcgtc agcctgcagg gggtgctgag ccccg
//
LOCUS       DAOSOA010000409         2427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_43412_length_2427_cov_2.307336, whole genome shotgun sequence.
ACCESSION   DAOSOA010000409 DAOSOA010000000
VERSION     DAOSOA010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43412_length_2427_cov_2.307336"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PKY13_16485"
     CDS             <1..906
                     /locus_tag="PKY13_16485"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT81593.1"
                     /translation="IYGLTIWVRIDRVDADLAGSAGRGTTYLLIGSDRRADIAEADRP
                     RFGSAGEVPGERADIVIVVNALADGTVRALSIPRDLVLFRQDRGPERLAPLLGDGPGA
                     IADALCNSLGIGVDHLAVIHFSGIDDLVDLTDGVVIEPSGTFLDRQSGLLVSAGRQRV
                     DGRTALAYVRARHIERREGDNWLPDPEESARRGDRALEVLRGLGSAVELGWNPWHDHR
                     IAWAVTDAVTVDDDMGIGDMMSLAEAVRGLRGASVESLPVLVRNGDVPTASITASAPA
                     AVQRLTGTEEPPPACSTPTLLSGTG"
     gene            908..2071
                     /locus_tag="PKY13_16490"
     CDS             908..2071
                     /locus_tag="PKY13_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81594.1"
                     /translation="MRVGAIALALVTLGLAIGCDGPDEPTAPTRSDRSTTTALDPVDV
                     AAGPIQRVRPPAESELATIDRDAGIAVTLRDNSVLWLFGDTAARLDDGTIDYFQIGTA
                     SWAPPHAPAVTDDIADPATGAPVTFATPTSDFPTCPGPGQRAGMWPASAVVQPVGERD
                     RVIIWLHNICLGADGSLTDEGMALAEWWYDPAAPPVERPVVATILVQRLFADRSFGTA
                     AVLGDDGDVYAYRCATPDDPADAAGYGPCAVARVPLADVADASAYTVVEDDLALPSGP
                     TQPIPAGGFGVAHDPVIGAFVMAYSPWPGSTQVVDVRTAPSPLGPWSRPVTVGLDGCA
                     DRIGSRQFNCYAGGPQPRFSTPGHLGLGYYDSSVSTFPTRGAYTVTSVPWPNS"
     gene            2082..>2427
                     /locus_tag="PKY13_16495"
     CDS             2082..>2427
                     /locus_tag="PKY13_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81595.1"
                     /translation="MRITPRHWHNETWICSMRGHVAPAATVAVLTDDDRRLGLVVNDV
                     RYVRCLRCDGWFECADPTPEQVTSERMPAHDELALPRRGKQLQDAIVLRLVAINKGLH
                     AVAFTLAALAIVA"
BASE COUNT          333 a          931 c          805 g          358 t
ORIGIN      
        1 atctacggcc tgaccatctg ggtccgcatc gaccgagtcg atgcggacct cgccggttct
       61 gcgggccgag gtaccaccta cctcctgatc ggctcggatc gccgggccga catcgccgag
      121 gccgaccgtc cgcggttcgg atcggccggc gaggttcccg gcgagcgggc cgacatcgtc
      181 atcgtcgtca acgccctcgc cgacggcacg gtgcgggcgc tgtcgatccc gcgtgacctc
      241 gtcctgttcc gccaggaccg ggggcccgag cggctcgctc ccctgctcgg cgatgggccg
      301 ggcgcgatcg ccgacgcact gtgcaattcc ctgggcatcg gggtcgacca cctcgcggtg
      361 atccacttca gcgggatcga cgacctcgtc gatctcaccg acggtgtggt gatcgaaccg
      421 agcgggacgt tcctcgatcg acagagcggc ttgctggtgt cggcaggccg gcagcgcgtc
      481 gacggccgaa ccgcgctcgc gtacgtgcgg gcacgccaca tcgagcgacg agagggcgac
      541 aactggctcc cggaccccga ggagagcgcc cgtcgcggcg atcgagcact cgaggtgctc
      601 cgtgggctcg gctcggccgt cgaactgggg tggaacccct ggcacgacca ccggatcgcg
      661 tgggccgtga ccgacgcggt caccgtcgac gacgacatgg gcatcggcga catgatgtcg
      721 ctggccgagg ccgtgcgggg gcttcgggga gcgtcggtcg agtcccttcc cgtgctggtg
      781 cgcaacggcg acgtaccgac ggcgtcgatc acggcgagcg ccccggctgc cgtgcagcgc
      841 ttgacaggca ccgaggagcc gccgccggcg tgcagcacgc cgacgctgct ctccggaacc
      901 ggctgacatg cgagtcggcg cgatcgcgct ggcactcgtg accctcgggc tcgcgatcgg
      961 ctgcgacggt ccggacgaac cgacggctcc gacccgctcc gaccgctcga ccaccactgc
     1021 gctcgacccg gtcgacgtcg cggccggacc gatccaacgc gtacggccac ccgccgagtc
     1081 cgaactcgcc accatcgacc gtgacgccgg catcgccgtc acgctccgcg acaactcggt
     1141 cctgtggttg ttcggcgaca ccgccgctcg cctcgacgac ggcacgatcg actacttcca
     1201 gatcggcacg gcatcgtggg ctccccccca cgctcctgcg gtcaccgacg acatcgccga
     1261 tccggcgacc ggggcgcccg tcacgttcgc cacgccgacg tccgacttcc ccacctgtcc
     1321 cggtccgggc cagcgggccg ggatgtggcc ggcgtccgcc gtcgtccaac ccgtcggcga
     1381 gcgcgaccgg gtcatcatct ggctgcacaa catctgcctc ggggccgacg gctcgctcac
     1441 cgacgaaggc atggcactgg cggaatggtg gtacgacccc gccgcccctc ccgtcgaacg
     1501 acccgtcgtg gcgacgatcc tcgtgcagcg gctctttgcg gatcggtcgt tcggcaccgc
     1561 cgccgtgctc ggcgatgacg gcgacgtgta cgcctatcgc tgcgccaccc ccgacgatcc
     1621 ggccgatgcc gccgggtacg gcccctgtgc cgtggcccgg gtcccactcg ccgacgtcgc
     1681 cgacgcgtcc gcctacaccg tcgtcgagga cgatctggcc ctgccgtccg ggccgacgca
     1741 accgattccg gccggcggct tcggtgtcgc ccacgacccg gtcatcggcg cattcgtcat
     1801 ggcgtacagc ccgtggccgg ggtcgaccca ggtcgtcgac gtccggaccg caccctcgcc
     1861 gctcgggccc tggagccgac cggtcacggt gggcctcgac ggctgcgccg accggatcgg
     1921 gagtcgccag ttcaactgct acgccggcgg cccacaaccc cggttctcga cgcccggcca
     1981 cctcggactc ggctactacg acagctcggt ctcgacgttc ccgacacggg gtgcgtacac
     2041 cgtgacgtcg gtcccgtggc ccaactccta gcctgcctcc gatgcgtatc actccgcgcc
     2101 actggcacaa cgagacctgg atctgctcga tgcgtgggca cgtcgcgccc gcggcgaccg
     2161 tcgcggtgct caccgacgac gaccgccggc tcgggctcgt cgtcaacgac gtccggtatg
     2221 tgcgctgcct ccggtgtgac ggttggttcg aatgcgccga cccgacgccc gagcaggtca
     2281 cgtccgaacg gatgcccgcc cacgacgagc tcgcgctccc ccgccgaggc aagcagctgc
     2341 aggacgcgat cgtcctgcgg ctcgtcgcca tcaacaaggg cctccacgcc gttgcgttca
     2401 cgctcgcagc gctcgccatc gtggcgc
//
LOCUS       DAOSOA010000410         2418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_43607_length_2418_cov_5.263225, whole genome shotgun sequence.
ACCESSION   DAOSOA010000410 DAOSOA010000000
VERSION     DAOSOA010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43607_length_2418_cov_5.263225"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..241)
                     /locus_tag="PKY13_16500"
     CDS             complement(35..241)
                     /locus_tag="PKY13_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81596.1"
                     /translation="MPWCDDCDRFYTPSTLTEDGKCPDGHQVADPDENAPSTKIPWHF
                     WLMVIALAIYLGWRLIQGIEWLLS"
     gene            complement(310..1491)
                     /locus_tag="PKY13_16505"
     CDS             complement(310..1491)
                     /locus_tag="PKY13_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81597.1"
                     /translation="MANAELRARRRSIESARALGVATAPEIDDSWSRCRPVIDASSAA
                     APVDAEPDEVEERWAASPIRRSEVGLEERLTQAADAGDLVAAVTDEEGRILWSAGGRS
                     MRRTAETVGFVPGGRWDESSAGTNALGLALRWGRPASVFSAEHWCDAVRDWVCWSAPV
                     RDRTGRPIGVIDLSGRWDRATPLAEVAVGTLARLVEEHLPDDDTPEPSGLRLRLLGHA
                     EATFDGQPLALGPRQMELLAALALEGPSTLDELRWHVYGDRPISPTTIKAEMSHLRQL
                     LGGGIGSRPYRLTVPVEVDVTTLRSELRSGDLGRVTAGYNGSLLVDSEAPFADDHRHV
                     FDVALRESLLARGRTPELLAFALVHPYDEAVLERAVAVAAAGSADHHEAVARLSLARR
                     G"
     gene            1608..>2418
                     /locus_tag="PKY13_16510"
     CDS             1608..>2418
                     /locus_tag="PKY13_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT81598.1"
                     /translation="MLFPAPGGEGSPITIKSRYDNFIGGKWVPPVDGRYFENPSPVDG
                     KTFCEVARGTAADIELALDAAHAAKDSWGRTSPAERANILIQIAQRIEDHLEEIAVVE
                     CWENGKSVRECLAADLPLAVDHFRYFAGVLRAEEGSITQIDDTTVAYHFNEPLGVVAQ
                     IIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPMSILHLTEKIADLLPDGVLNIVNG
                     FGVEAGKPLASSPRVAKVAFTGETTTGRLIMQYASENLNPVTLELGGKSANI"
BASE COUNT          387 a          856 c          837 g          338 t
ORIGIN      
        1 gggagcgagc ctgagcggag gtggccgagc ccagttaaga gagcagccat tcgatgccct
       61 ggatgaggcg ccaaccgagg tagatcgcga gtgcgatcac catcagccag aagtgccagg
      121 ggatcttggt gctgggcgcg ttctcgtcgg gatcggcgac ctggtgaccg tcggggcact
      181 tgccgtcctc ggtcagggtg ctcggtgtgt agaaccgatc gcagtcgtcg caccagggca
      241 tcgccgcact gtctacaccc gcgcccattc cggtttcgaa cccgggttcg gctcgccgct
      301 gaggcggtct cagccgcgtc gggcgaggct gaggcgggcg acggcctcgt ggtggtcggc
      361 cgagccggcc gctgccaccg ccaccgcccg ctcgaggacc gcctcgtcgt acggatggac
      421 gagggcgaag gccagcagct ccggcgttcg accacgcgcc aacaacgatt cgcggagcgc
      481 gacgtcgaac acgtgtcgat ggtcgtcagc gaacggcgcc tcgctgtcga cgagcagcga
      541 cccgttgtag ccggcggtca cccgaccgag gtcgcccgac cggagctccg accgcagggt
      601 cgtcacgtcg acctcgacgg gaacggtgag gcggtacggc cgggacccga tgccaccgcc
      661 gagcagctgt cggaggtgcg acatctcggc cttgatcgtg gtggggctga tgggacggtc
      721 gccgtagacg tgccatcgca gttcgtcgag ggtactcggg ccctcgaggg cgagggccgc
      781 cagcagttcc atctggcgcg ggccgagcgc gagcggctgg ccgtcgaacg tcgcctcggc
      841 gtggccgagc agccgcagtc ggaggcccga gggctcggga gtgtcgtcgt cgggcaggtg
      901 ctcctcgacg aggcgggcca gcgtgccgac ggcaacctcg gcgagcgggg tggcgcggtc
      961 ccaccggccg gacagatcga tgaccccgat cgggcgaccg gtgcgatcgc gtaccggcgc
     1021 cgaccagcac acccagtcac gcaccgcatc acaccagtgc tctgcggaga acacactcgc
     1081 cgggcgcccc caccggagcg cgaggcccaa ggcgttggtg ccagcagacg actcgtccca
     1141 gcgcccaccg ggcacgaacc ccacggtctc ggcggtccgc cgcatgctgc gccccccggc
     1201 gctccacagg atccggccct cctcgtcggt cacggcggcg accaggtcgc cggcgtcggc
     1261 cgcctgggtc agccgttcct cgagaccaac ctcgctgcgg cggatcggcg acgccgccca
     1321 gcgctcctcg acctcgtcgg gctcggcgtc gaccggcgca gcggcgctcg acgcgtcgat
     1381 cacggggcga cagcgcgacc acgagtcgtc gatctcgggg gcggtcgcca ccccgagggc
     1441 gcgcgccgac tcgatcgatc gacgtctggc gcgcagttcc gcgttcgcca ccgaaacccc
     1501 cttggttcgt ctcgaaccgt accaaccttc tgccaaccgc gggcggcgca cagtgaggag
     1561 gagaccgcgc agggggtgcg gtccacgatc cctcgaggag tccgaccatg ttgttcccag
     1621 cacccggtgg cgaaggcagc cccatcacga tcaagagccg ctacgacaac ttcatcggcg
     1681 gcaagtgggt cccgccggtc gacggtcgct acttcgagaa cccgtcaccg gtggacggca
     1741 agacgttctg cgaggtcgct cgtggaaccg cggccgacat cgagctggca ctcgacgccg
     1801 cgcacgcggc gaaggactcg tggggccgaa ccagcccggc cgaacgggcc aacatcctca
     1861 tccagatcgc ccagcgcatc gaggaccatc tcgaggagat cgccgtcgtc gagtgttggg
     1921 agaacggcaa gtcggtgcgc gagtgcctcg ccgccgacct gccgctcgcc gtcgaccact
     1981 tccgctactt cgccggcgtc ctgcgcgccg aggaggggtc gatcacgcag atcgacgaca
     2041 cgaccgtcgc ctaccacttc aacgaaccgc tcggtgtggt ggcgcagatc atcccgtgga
     2101 acttcccgat cctcatggcc gtgtggaagc tcgctcccgc gctcgccgcg ggcaactgcg
     2161 tggtgctgaa gcccgccgag cagaccccga tgtcgatcct gcacctgacc gagaagatcg
     2221 ccgacctgct tcccgacggt gtgctcaaca tcgtgaacgg gttcggggtc gaggccggca
     2281 agccgctcgc atccagccct cgggtcgcga aggtggcgtt caccggcgag acgacgaccg
     2341 gccggctcat catgcagtac gcctcggaga acctgaaccc ggtgacgctc gagctcggcg
     2401 gcaagagcgc caacatct
//
LOCUS       DAOSOA010000411         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_43743_length_2413_cov_8.460136, whole genome shotgun sequence.
ACCESSION   DAOSOA010000411 DAOSOA010000000
VERSION     DAOSOA010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43743_length_2413_cov_8.460136"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..1038
                     /locus_tag="PKY13_16515"
     CDS             322..1038
                     /locus_tag="PKY13_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81599.1"
                     /translation="MIESTVAVGDTISVRAGVPRSYGRPLGRVVRINATTVTVALFYD
                     LVRGDRTTRLVPHTGYRLAHRPDPRPWVVVGCGARKVEHPAPIADLYTGSYHAQCVRA
                     ATRLTDRSRVVILSGRYGLVRLTDPTVREPYEQRIDQPGAVTTDELHTQGDRLGLSWP
                     LERVIVLAGKAYTAPLLTVVDHRNLELPFDGTRGIGDHKALLRLIETTGTLPRRESPS
                     TTPQEVHEHDRTHQLHRLVH"
     gene            995..1300
                     /locus_tag="PKY13_16520"
     CDS             995..1300
                     /locus_tag="PKY13_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81600.1"
                     /translation="MSTIERTNSTASFTERHGELVHLTVTTTTWSNGDTDVFERATTL
                     HGGRVETNTRNGRLHDLPSGTAAFRILGADGTLVSETHFAPRPGGAIRSIFRRLQRA"
     gene            1409..1945
                     /locus_tag="PKY13_16525"
     CDS             1409..1945
                     /locus_tag="PKY13_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81601.1"
                     /translation="MANASKNKGDRFEREAAAYLLEHAADIVLPDCQRLLGAGRKDDI
                     GDLRAFVDVAVQVKAYNNVLAALREGVAGARAQAERSGTELHLAMVPIPRVSRTNPDV
                     VRWLACSYVWPTPVTTDTFAMSGRALTWVRTADEPIDTRVATIATRGLEPVYLGSLQA
                     WLAAYRNRGKIAAQTPSN"
     gene            1953..>2413
                     /locus_tag="PKY13_16530"
     CDS             1953..>2413
                     /locus_tag="PKY13_16530"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT81602.1"
                     /translation="MGTPDSYVSLHNHTEHSTLDGLSRLNELCEAAAADGSPAVGITD
                     HGTMSGTFKFAAAARKAGVKPLMGFEAYLAIGSRHDQNSIEIPRDDAESDADDDGTGK
                     GSTKVKRYEHITLLASDTDGWANLCKLTSFSHDSFWYKPRIDFELMAEHNRG"
BASE COUNT          460 a          947 c          683 g          323 t
ORIGIN      
        1 accgccagct cgccgaagca cgctccacga tcgactggct cggtgcggga tcgtcctcga
       61 ccctgcgaac ccgacgatga tccagccgcg catcctccgg cgccgcaccg acaagacggc
      121 aaccgaatgc caccttgccc agttcgcgac cgccgggacc aggcgatgac gcccgaccgc
      181 cgccccgaag ctgcaacggt cgacgagatc gaagccgcct ccgcgagatc gacgccgcca
      241 tgcgccgagg agacaacgac gcagcactcg agctgtcgac cggcctcaac gagtaccgga
      301 cccacctcaa ggcctgacgc tgtgatcgaa agcacagtcg ccgtcggcga cacgatcagc
      361 gtgcgcgccg gcgtcccgag gtcctacggg cgtccgctcg gacgcgtcgt ccgcatcaac
      421 gccaccaccg tcaccgtcgc gctgttctac gacctcgtca gaggtgaccg caccacccgg
      481 ctggtccctc acacgggata ccgcctcgcc caccgtcctg atcctcggcc gtgggttgtc
      541 gtcgggtgcg gcgcccgcaa agtcgaacac ccagcaccga tcgcagacct ctacaccggc
      601 tcctaccacg cccagtgcgt cagggcggca acgcgactca ccgaccgttc gcgggtcgtc
      661 atcctgtcgg gccgctacgg gctcgtacgt ctcaccgacc cgacagtcag ggaaccctac
      721 gaacagcgca tcgaccagcc cggcgccgtt accaccgacg agctccacac ccagggcgac
      781 cggctcgggc tcagctggcc cctcgaacga gtcatcgtgt tggccggcaa ggcctacacg
      841 gcgccgcttc tcaccgtcgt cgaccatcgc aacctcgaac tccccttcga cggcacccgc
      901 ggcatcggcg accacaaggc cctcctgcga ctgatcgaga ccaccggcac gctcccccgg
      961 cgggaatcgc cttcgaccac cccacaagaa gtgcatgagc acgatcgaac gcaccaactc
     1021 caccgcctcg ttcactgaac gccacggtga gctcgtccac ctcaccgtca ccaccaccac
     1081 gtggtccaac ggcgacacgg acgtgttcga acgcgccacc acgcttcacg gcgggcgagt
     1141 cgagaccaac actcgcaatg gtcgactcca cgacctcccg tcgggcactg cggcctttcg
     1201 gatcctcggg gcagacggca ccctcgtgtc cgaaacccac ttcgctcccc gtccgggcgg
     1261 agccatccga tcgatcttcc gccgcctcca gcgagcgtga cgaacgaatc cggcaccgtc
     1321 ggcgcagcga ccacccggcc cgccgctcat cccggtcgga accgcaccca cagcgccata
     1381 gccgccccga cgttggcata gaccggtcgt ggccaacgca tcgaagaaca aaggggaccg
     1441 gttcgaacgc gaggccgcgg cctacctgct cgagcatgcc gccgacatcg tcctccctga
     1501 ctgccaacgg ctcctcggag ccggccgcaa agacgacatc ggcgacctgc gcgccttcgt
     1561 tgacgtggcc gtccaggtga aggcgtacaa caacgttctc gccgctcttc gcgaaggagt
     1621 agccggagcg cgcgctcagg ccgaacgctc gggcaccgag ctgcaccttg cgatggtccc
     1681 gattcccagg gtgtcgcgca ccaaccccga cgtcgtccga tggctggcct gctcctacgt
     1741 gtggccaacc cccgtcacca ccgacacgtt cgcgatgtca ggtcgcgccc tcacctgggt
     1801 acgcaccgcc gacgaaccga tcgacacccg ggtcgcgacc atcgccaccc gcggcctcga
     1861 acccgtctac ctcggatcgc tccaagcctg gttggcggcg taccgcaacc ggggcaagat
     1921 cgccgcgcag acgcccagca actgagcgaa cgatgggcac tccggattcc tacgtcagcc
     1981 ttcacaacca caccgagcac tcgaccctgg acgggctgag ccgcctcaac gagctgtgcg
     2041 aggcagctgc ggccgacgga agcccagcgg tcggcatcac cgaccacggc accatgtccg
     2101 gcacgttcaa gttcgccgcg gcagccagaa aggccggcgt caaaccgctc atgggcttcg
     2161 aggcatacct ggcgatcggg tcgcgccacg accagaactc gatcgagatc ccccgcgacg
     2221 acgccgaatc cgacgccgac gacgacggca ccggaaaggg ctccacgaag gtcaaacgct
     2281 acgagcacat caccctcctc gcgtcggaca ccgacggatg ggcgaacctg tgcaagctca
     2341 cctccttttc ccacgactcg ttctggtaca agccgcgcat cgacttcgag ctgatggccg
     2401 aacacaaccg cgg
//
LOCUS       DAOSOA010000412         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_44490_length_2385_cov_4.935622, whole genome shotgun sequence.
ACCESSION   DAOSOA010000412 DAOSOA010000000
VERSION     DAOSOA010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44490_length_2385_cov_4.935622"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKY13_16535"
     CDS             complement(<1..333)
                     /locus_tag="PKY13_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81603.1"
                     /translation="MTLIEATRDEEAMSAKADAIRELAAHLHAANARLAELLADFTTD
                     DTWNVSGDWRSLPHWLGVNCGYARAEANILARVATAASAMPTVMERATRGEVSLHVAA
                     AAARVATPD"
     gene            491..2062
                     /locus_tag="PKY13_16540"
     CDS             491..2062
                     /locus_tag="PKY13_16540"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOT81604.1"
                     /translation="MSRRDLLKALGIGVPGAYLLSGCAPSVSPTFPEPNHSDPDPVPF
                     VDGVIAGDPRPDGAVIWTRVQAGPGPIGVLWSVATDPSFASVVAGGVVTAEASGDHAV
                     HVLVDGLQSDSWYWYRFEADGVASRTGRLRTAPAPGATVDSMRFAFSSCQQLNPSWFV
                     AHRAAAAEPDLDFFMHLGDYVYVSDTGTQTLDDYRDVYHRWRRDPLLREFHATVPMVA
                     MWDDGEFYNGVDKYGPPARLAAGRQAWFENMPILNDGADTTYRNVAWGSLADIPMIDV
                     RSHRDPYLESGDFTQGTGLTAYDPNRSQFGPEQYQWLVDTLANSTADWRIVGNGVPIS
                     PWRLVNLEFLRPFRDGLPPNAGIYIRSDGWDDYMVERRGLLQAILDAGVDNTVFATGQ
                     THIAIASELRTDPDAGGPVAAFEFVSQSMTADPDIKKSLLGGLPSEAASAALRLAENW
                     LMGQNPGMRHLNFEQQGYVVVDVNPDEMIVTYRHIDTYDPDAEAWTGATFRVANGGQH
                     IEVLQRRSDAGSVAP"
     gene            complement(2075..>2385)
                     /locus_tag="PKY13_16545"
     CDS             complement(2075..>2385)
                     /locus_tag="PKY13_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HOT81605.1"
                     /translation="FHQSGGCCDGSSPMCFPLGEFRIGGSDVLLGEVHGAPFHMSSEQ
                     FEYWSHTHLTLDVVPGRGGGFSLETPEGVRFLIRSRLFTDDELADLPPVHVGPVAAEQ
                     "
BASE COUNT          364 a          848 c          800 g          373 t
ORIGIN      
        1 gtcgggggtg gcgacgcggg cggcggccgc ggccacgtgc aaggacactt cgccgcgcgt
       61 ggcgcgttcc atgacggtcg gcattgcgct ggcggcggtg gcgacgcggg cgaggatgtt
      121 ggcttcggcg cgggcgtatc cgcagttgac gcccagccag tgggggagtg accgccagtc
      181 gcccgagacg ttccaggtgt cgtcggtggt gaagtcggcg agcagttcgg cgagtcgtgc
      241 gtttgccgcg tggaggtgcg cggcgagttc gcggatggcg tcggcttttg ccgacatcgc
      301 ctcttcgtcg cgtgtcgcct cgatcaaggt cacgggctga actctaccat gcaaacgaac
      361 accagttcga tcaagcggga agaatttccc tgcttccgtg ggggagctcg cccacctacg
      421 atctgtccag gcgagctggg gaggacagat ggcggggtat cccgaacgag tagtggccgg
      481 gcgcggaggc atgagccgcc gcgatctgct gaaggcgctg ggcatcggcg tccccggtgc
      541 gtacctgctc tcggggtgcg ctccgtcggt gtcgcccacc ttcccggagc cgaaccactc
      601 cgatcctgac cccgtcccct tcgtcgacgg cgtcatcgcc ggtgaccccc gccccgacgg
      661 tgccgtgatc tggacgaggg tccaggcggg ccccggtccg atcggggtct tgtggagcgt
      721 ggcgaccgac ccctcgttcg cctcggtggt ggccggtggc gtcgtcaccg ccgaggcgtc
      781 aggcgaccac gccgtgcacg tgctcgtcga cggcctgcaa tccgacagct ggtactggta
      841 ccgcttcgag gccgacggtg tggcgtcccg caccggccgg ctgcgtaccg ctccggctcc
      901 cggcgccacc gtcgactcga tgcggttcgc gttctcgagc tgccagcagc tcaaccccag
      961 ttggttcgtc gctcaccgcg ccgccgccgc cgaacccgac ctcgacttct tcatgcacct
     1021 gggtgactac gtctacgtca gcgacaccgg cacccagacc ctcgacgact accgcgacgt
     1081 ctaccaccgc tggcgtcgcg acccgctgct gcgggagttc cacgccaccg tgcccatggt
     1141 cgccatgtgg gacgacggcg agttctacaa cggtgtcgac aagtacggcc cgcccgcccg
     1201 gctcgccgcc ggccggcagg cgtggttcga gaacatgccg atcctcaacg acggcgccga
     1261 caccacctac cgcaacgtcg cgtggggcag cctcgccgac atcccgatga tcgacgtccg
     1321 atcccaccgc gacccgtatc tcgagagtgg cgacttcacg cagggcaccg ggctcacggc
     1381 gtacgacccg aaccgctcgc agttcgggcc cgagcagtac cagtggctgg tggacacgct
     1441 ggcgaattcg accgctgact ggcgcatcgt cggcaacggc gtaccgatca gcccgtggcg
     1501 gctcgtgaac ctcgagttcc tccgtccctt ccgcgacggc ctcccgccga acgccggcat
     1561 ctacatccgc agcgacgggt gggacgacta catggtcgag cgccgggggc tcctccaggc
     1621 gatcctcgac gccggtgtcg acaacacggt gttcgccacc ggccagaccc acatcgccat
     1681 cgcgtccgag ctgcgcaccg atcccgatgc cggcggcccg gtggcggcgt tcgagttcgt
     1741 gtcgcagtcg atgaccgccg atcccgacat caagaagtcc ttgctcggcg ggttgccctc
     1801 cgaggcggct tcggccgcgc tgcggctcgc cgagaactgg ctgatgggcc agaaccccgg
     1861 catgcgccac ctcaacttcg agcagcaggg ctacgtcgtc gtcgacgtca accccgacga
     1921 gatgatcgtg acgtaccgcc acatcgatac gtacgacccc gacgccgagg cgtggaccgg
     1981 agcgacgttc cgcgtggcga acggtggcca gcacatcgag gtcctccagc gccgcagcga
     2041 cgccggatcg gtcgcccctt agtcggagcg accgctactg ctcggctgcc accggtccca
     2101 cgtggaccgg tggcaggtca gcgagctcgt cgtcggtgaa caggcgggac cggatcagga
     2161 accggactcc ctcgggcgtc tcgagcgaga acccaccgcc gcggcccggg acgacatcga
     2221 gggtcaggtg ggtgtggctc cagtactcga actgctccga gctcatgtgg aacggcgccc
     2281 cgtgcacctc tcccaacagc acgtcggacc caccgatgcg gaactcgccg agcgggaagc
     2341 acatcgggct cgacccgtcg cagcaaccgc ccgactggtg gaaca
//
LOCUS       DAOSOA010000413         2381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_44605_length_2381_cov_5.509458, whole genome shotgun sequence.
ACCESSION   DAOSOA010000413 DAOSOA010000000
VERSION     DAOSOA010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44605_length_2381_cov_5.509458"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKY13_16550"
     CDS             <1..467
                     /locus_tag="PKY13_16550"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HOT81606.1"
                     /translation="PERERVIEQRPALDDALAVRLAGRAAEEIVFGVASTGAHDDLVQ
                     ATELARRMVREWGMSPKLGHLAWGSNSAVFLGEDLVQTRDYSDETARLIDSETEKILE
                     AQAARARDCLERYRAALDAIAAALVERETLSGADVADLVEAAHAPRLAGIRP"
     gene            464..1297
                     /locus_tag="PKY13_16555"
     CDS             464..1297
                     /locus_tag="PKY13_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81607.1"
                     /translation="MTESPSTARGTTRRRVVLGVVAALAVAITVVVGIVVWGGSSGHE
                     VSSDEAGRRIGTATSIAPVQGVLRPEQGVYRYRGSGTDALSTPPKEQTEGPDMPATVT
                     HRSDGCWTFRIDFHSNHWQSWEYCATDGILDERGGSTFQRWDFGVFANETTSTFACDS
                     SVTIRLDAEPGDEWTQTCHSEPTDDPAASRSAGPYRFVGAETLTIGGEPVEALHYHRE
                     RTMSGGQDGTEQADVWFAAADGLPLRNERRIEVRTDTIIGETTYTEDAEFELQSIEPE
                     A"
     gene            1407..1730
                     /locus_tag="PKY13_16560"
     CDS             1407..1730
                     /locus_tag="PKY13_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOT81608.1"
                     /translation="MIDKKFQRRGGRVKVTFSIPTEWLDRPTSVVGDFNDWDPTATPL
                     RKKGSLRSATVVLDEGGQYAFRYLDSDGRWHDDPAADAISPNADGGTNCVVDLRRPQP
                     DAATD"
     gene            complement(1756..>2381)
                     /locus_tag="PKY13_16565"
     CDS             complement(1756..>2381)
                     /locus_tag="PKY13_16565"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOT81609.1"
                     /translation="VMRKLVVSEFVSLDGIVEAPGGEDGYRHGGWTIPYFSPELGEYK
                     TAELLAADALLLGRVQYEGFAEAWPERSGDPMSDHMNAMAKYIVSASLQDPAWNNTHL
                     LGGDMVPAVTALKETEGADILVAGSATLVRSLLEADLVDELRMAIYPLTLGSGKRLFP
                     DDSHLHFELVEVTTIPTGVMLASYRRSEAPVTVEFDYGSLVEPTSES"
BASE COUNT          389 a          831 c          802 g          359 t
ORIGIN      
        1 tgcccgagcg tgagcgggtc atcgaacaac gccccgccct cgacgacgcc ctcgccgtac
       61 ggctcgccgg tcgcgccgcc gaggagatcg tcttcggcgt cgcgtcgacc ggggcccacg
      121 acgacctcgt tcaggccacc gagctcgccc gtcgaatggt ccgcgagtgg ggcatgtcac
      181 ccaaactcgg ccacctcgcc tggggatcga actctgcggt cttcctgggg gaggacctcg
      241 tgcagacccg cgactactcc gacgagacgg cgcggctcat cgactccgag accgagaaga
      301 tcctcgaggc ccaggcggct cgcgcccgcg actgcctgga gcggtaccgc gctgcgctcg
      361 acgccatcgc cgccgcgctc gtcgaacgcg agacgctgag cggggccgat gtcgccgacc
      421 tcgtcgaggc cgcgcacgca ccgcggctcg ccggcatccg gccgtgaccg agtcgccgtc
      481 gaccgccagg ggcaccaccc ggcgccgggt cgtgctcggc gtcgtcgctg cgttggccgt
      541 ggcgatcacc gtggtcgtcg gcatcgtggt gtggggtgga tcgtccggcc acgaggtgag
      601 ttccgacgag gccggccggc gtatcggcac ggccacgtcg atcgccccgg tgcaaggtgt
      661 gctccggccg gagcagggcg tctaccgcta ccggggcagc ggtaccgacg cgctcagcac
      721 tccacccaag gaacagaccg agggccccga catgccggcg acggtcacgc atcgctccga
      781 cggatgctgg acgttccgga tcgacttcca ctcgaatcac tggcagtcgt gggagtactg
      841 cgccaccgac ggcatcctcg acgagcgggg cggctcgacc tttcaacggt gggacttcgg
      901 cgtcttcgcc aacgagacca cgtcgacgtt cgcctgcgac tcgtcggtga cgatccgact
      961 cgacgccgaa cccggcgacg agtggacgca gacctgtcac agcgaaccga ccgacgatcc
     1021 ggccgcgtca cggagcgccg ggccctaccg attcgtcggt gccgagacgc tgacgatcgg
     1081 aggcgagccg gtcgaagcgc tccactacca ccgtgagcgc accatgtcgg gagggcagga
     1141 cggcaccgag caggccgacg tgtggttcgc cgctgcggac ggactgccgc tgcgaaacga
     1201 acggcgcatc gaggtgcgca ccgacacgat catcggcgag accacctaca ccgaggacgc
     1261 cgagttcgag ctgcagtcga tcgaaccgga ggcctgagcg ggccaccccg tacgccggtc
     1321 gggcccttgg tccctggtcg gtcgggggat ccggttgcac gatctatcta cggacgggca
     1381 tcgacccgga gtgacaggag agcatcgtga tcgacaagaa gttccagcgg cgcggtggtc
     1441 gcgtgaaggt cacgttctcc attccgaccg aatggctgga tcggccgacg tcggtggtgg
     1501 gtgacttcaa cgactgggat ccgacggcga ccccgcttcg caagaagggg tccctgcgga
     1561 gcgccacggt cgtcctcgac gagggtggcc agtacgcgtt ccgctacctc gacagtgacg
     1621 gtcgttggca cgacgatccc gccgccgacg cgatctcgcc aaacgcggac ggtgggacca
     1681 actgcgtcgt cgacctgcgt cgcccccaac ccgacgcggc gaccgactga cacgtgatcg
     1741 ccggtgtgcc gccggtcagc tctccgacgt cggctccacc aaggacccgt agtcgaactc
     1801 gacggtgacc ggagcctcgg accgtcggta cgaggcgagc atcactcccg tcggaatcgt
     1861 ggtgacctcg accagctcga agtgcaggtg actgtcgtcg gggaacagcc gttttcccga
     1921 tcccagcgtc agcgggtaga tcgccattcg gagttcgtcg accaggtcgg cctcgagcag
     1981 cgagcgcacg agggtcgctg agccggcgac gaggatgtcc gccccctcgg tctccttcag
     2041 cgcggtcact gctggcacca tgtcgccgcc caacaggtgt gtgttgttcc aggccggatc
     2101 ctgcagtgac gccgacacga tgtatttcgc catggcgttc atgtggtcgg acatcggatc
     2161 gccggagcgc tccggccacg cttcggcgaa tccctcgtac tggacccggc cgagcagcaa
     2221 cgcgtcggct gccaacagct cggccgtctt gtactcgccc aactcggggc tgaagtaggg
     2281 gatggtccag ccgccgtggc ggtagccgtc ctcacccccg ggggcctcga cgatcccgtc
     2341 gagcgatacg aactccgaga cgaccagctt cctcatgaca t
//
LOCUS       DAOSOA010000414         2369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_44924_length_2369_cov_4.202247, whole genome shotgun sequence.
ACCESSION   DAOSOA010000414 DAOSOA010000000
VERSION     DAOSOA010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44924_length_2369_cov_4.202247"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKY13_16570"
     CDS             complement(<1..402)
                     /locus_tag="PKY13_16570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT81610.1"
                     /translation="MAVVESDVNVLWSRYQEAGDPGIRDALILNYSPLVKYVASRVSV
                     GLPQNVEHADLVSYGMFGLIDAIDKFEPDRGFKFETYAISRIKGAILDELRSIDWVPR
                     SVRSKVRSVEKAFAKLEAREGRAPTDEEVAAE"
     gene            complement(423..593)
                     /locus_tag="PKY13_16575"
                     /pseudo
     CDS             complement(423..593)
                     /locus_tag="PKY13_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(649..1374)
                     /locus_tag="PKY13_16580"
                     /pseudo
     CDS             complement(649..1374)
                     /locus_tag="PKY13_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1454..>2369)
                     /locus_tag="PKY13_16585"
     CDS             complement(1454..>2369)
                     /locus_tag="PKY13_16585"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT81611.1"
                     /translation="LVEAHAAAGITGTLHGDRGHPSIDRRDPHVPPVLLWRGGGSPAE
                     LTGRPSVGIIGTRRATRAGVELARDFGRSLALAGCTVVSGLALGVDAAGHRGALEAGA
                     APPLGVVGSGLDVVYPRANRALWHDVAERGLLCSEYPLATPPAAWRFPARNRILVALC
                     DVVVVVESHERGGSLITAGIAGARGVPVLAVPGALRSPASVGTNRLIADGCQPCLGVE
                     DVLAALGLTTAPTLPFPDQDASAVPDDRASPAEAEVLDALGWVARSVDELADDCPTLG
                     LAELSLAVASLLGRRIVVERDGRLERAR"
BASE COUNT          382 a          831 c          839 g          317 t
ORIGIN      
        1 ctcggcggcg acctcttcgt cggtgggcgc ccggccctcg cgagcttcga gcttcgcgaa
       61 cgccttctcc accgaccgga ccttggagcg aaccgagcgg ggcacccagt cgatgctgcg
      121 caactcgtcg aggatggcgc ccttgatacg gctgatggcg taggtctcga acttgaagcc
      181 acggtcgggc tcgaacttgt cgatggcgtc gatcaggccg aacatgccgt agctcacgag
      241 gtccgcgtgc tcgacgttct ggggaaggcc gaccgagacg cggctcgcga cgtacttgac
      301 caacggtgag taattcagga tgagggcgtc acggatgccc ggatcccccg cctcctggta
      361 ccgcgaccag aggacgttca cgtcgctctc tactactgcc atgtccgact tccccagcat
      421 tgtcacgccc tcggatgagt tccttcgtag acctgtcgca atcgctcctt ggagacgtgg
      481 gtgtagatct gcgtcgttgc cagatccacg tgtccgagaa gttcctgtac gactcttaga
      541 tcggcaccac cgtcgagaag atgagtcgcg aagctgtgcc gaagggcgtg ggggttcacg
      601 ggcgcctggg cttggcgcag cgtgcggcga tcgaggatcc tgcacgtccc gtggggcgag
      661 agcccgccca cgcaggttga agaacagata cgacgggtcg gcagcggccg agccgtcggt
      721 gggttcgaag cgggcccgac cccgggcgtt ccagcggtgg atcgcatcga tggcgggcac
      781 gctcagcggg acgaggcgct gcttgccgcc cttgccccag accgtggccg caccacgcgc
      841 caggtcgacg tcatcgacgc ggagcccgca gagctccgac acgcgcaggc ccgacccgta
      901 gagcagttcg agcacggcgt cgtcgcggag gtcgcgggcc tcgtcgtcgg gagacggcgc
      961 cgtggtaccg ccgttcggcg ccaccacggg tgcctcgtcg aggaggaccg tcagctggtc
     1021 agcggtcagc acacggggaa gccgacggtc gccgcgcggg gtggacaggc cggcggtggg
     1081 atcgacgtcg atgacccctt gggcggcgag ccagtcgaag tagcggcgaa gggccgagat
     1141 cttgcggcga atcgtacgcg gggcgagcgg gcgatcggct gctgccgggc caccctggcc
     1201 actcatcgct gccaggtacc gccggagcac cgcgcgggtc accgcctccg gctcgacgat
     1261 gtcctgcaga tcgagccagg cgacgaacgc gtcgagatcc cgccggtaga ccgcgcgggt
     1321 cgagtcgctc aacgagagcg tcgaacgctc gaactccgtc agccgccaac ccatggggcg
     1381 atggtacacc tccaacgctg acagcgcgaa cgagaccgct caccgcgcga ggcagggcgg
     1441 ggatctcgca cactcaccgc gctcgttcga ggcgcccgtc gcgctccacc acgatccgac
     1501 ggccgagcag cgacgccacg gcgagcgaca actcggcgag gccgagtgtc gggcagtcgt
     1561 cggccaactc gtccaccgac cgagccaccc atcccaacgc gtcgagcacc tccgcctcgg
     1621 ccggagacgc tcggtcatcg gggacggccg acgcgtcctg gtcggggaac ggcagggtcg
     1681 gagcggtcgt gaggccgagc gccgccagca cgtcctcgac gccgaggcag ggctggcagc
     1741 cgtcggcgat cagacggttg gtgccgaccg atgccggact gcgcaacgcc ccgggcaccg
     1801 cgagcaccgg cacaccacgg gcgccggcga tgcccgcggt gatgagcgag ccgccgcgct
     1861 cgtggctctc caccacgacc accacgtcac acaacgccac gagaatgcgg ttgcgggccg
     1921 gaaaccgcca cgctgccggc ggggtcgcca gcgggtactc cgagcacaac agaccgcgct
     1981 cggccacgtc gtgccagagc gctcggttgg ctcggggata cacgacgtcg agcccgctgc
     2041 cgaccacacc gagcggcggc gcggcgccgg cctcgagtgc tccccggtga ccggcagcgt
     2101 cgactccgag cgccagaccc gacaccacgg tgcacccggc gagggcgagc gagcggccga
     2161 agtcacgcgc cagttcgaca ccggcccgcg tcgcccgacg agtgccgatg atgccgaccg
     2221 atgggcgccc ggtgagctcc gccggcgacc ctccgcctcg ccacagcagc accggtggca
     2281 cgtgggggtc gcgccggtcg atgctcgggt ggccccggtc gccgtggagc gtgccggtga
     2341 tcccggccgc ggcatgagct tcgacgagg
//
LOCUS       DAOSOA010000415         2366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_44995_length_2366_cov_4.412376, whole genome shotgun sequence.
ACCESSION   DAOSOA010000415 DAOSOA010000000
VERSION     DAOSOA010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44995_length_2366_cov_4.412376"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1797)
                     /locus_tag="PKY13_16590"
     CDS             complement(133..1797)
                     /locus_tag="PKY13_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOT81612.1"
                     /translation="MELNIADICDALSVAYPGRECLTFRDRRLTWADVADRTNHLAAI
                     FGAAGLGRTRPFAGADGWESTQDHVALYLHNGNEYLEGMLGAYKAVCAPVNVNYRYRA
                     TELAYVLGDSGAKAIIFHSAFAPLLAEVLPDLPSLTLLLQVADSSGNPLLADVDGRSA
                     RWYEEALATAAPEARPLGERSGDDLYLCYTGGTTGMPKGVLWRQADFVVSALGVRRAD
                     GSDYESIDEIVTAAERSSLRALPAPPLMHGAAHWNAISCWIAGGTVIIQDHPEHLDPA
                     DIWSTVERHRATSLLIVGDSFARPLLDELARRADAGAPYDVSSMRHLLSGGAVLSPHV
                     KAELLDAVPGLTIVDVLGSSESGRQGVASTRTAEDSAAGFVRSSTARVVREDLSGALE
                     PGTGEVGWLTQTGRVPLGYLGDPDKTARTFPIIDGERHAVAGDRARLAADGSIELLGR
                     DSVCINTGGEKVFAEEVEVALTSHPDVFDAVVCGRPSERWGQEVVGVVALTPGSTTTA
                     TELIEHCKDRLAGFKAPKAIVFVDRVVRSPSGKADYRWAATQISPT"
     gene            1839..>2366
                     /locus_tag="PKY13_16595"
     CDS             1839..>2366
                     /locus_tag="PKY13_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT81613.1"
                     /translation="MADEPTESTVLLDTVEPGITRITLNRPSSLNSMNRQLIDELHAA
                     FDVVDADPDVRVVLLTGAGRGFCAGLDLRDPTLLMGRNDPEGPGVPQSGMALQQRIAS
                     LIPKMRRLRVPIIAAVNGPATGGGLALALGSDVRIAAEAARFGVAFIRIGLSACDIGV
                     SWALPRLVGASRAHEL"
BASE COUNT          344 a          810 c          852 g          360 t
ORIGIN      
        1 ctgtgggcaa ccagggcagc atcgttgggt gttgcctgtc gattgcctgg ggacgaccgc
       61 ccttccctgg ggacgaccga cttttgcggg tacctgtcct cacctgtcgg ggacaggtac
      121 ccgcgaaacc tgtcaggtcg gggagatctg ggtcgcggcc cagcgatagt cggccttgcc
      181 cgaggggctt cggacgaccc gatcgacgaa cacgatggcc ttcggtgcct tgaagccggc
      241 gaggcggtcc ttgcagtgct cgatcaactc ggtggccgtc gtcgtcgatc ctggcgtgag
      301 cgccaccaca cccacgacct cctggcccca ccgctccgac ggacggccgc acacgacggc
      361 gtcgaacacg tcgggatggg aggtgagggc tacctcgacc tcctcggcga acaccttctc
      421 gccgccggtg ttgatgcaca ccgaatcgcg acccaggagc tcgatcgagc cgtccgcggc
      481 aagtcgggcg cggtcgccgg ccacggcgtg gcgttcgccg tcgatgatcg ggaaggttcg
      541 agcggtcttg tcgggatcgc cgaggtagcc gagcggcacc cggccggtct gggtcagcca
      601 cccgacctcg ccggtacccg gttcgagcgc tccgctcagg tcctcgcgca ccacccgggc
      661 cgtcgaggat cggacgaacc cggctgccga atcctcggcc gtccgcgtcg aggccacgcc
      721 ctggcgcccg ctctccgacg aacccagcac gtcgacgatg gtcagcccag ggaccgcgtc
      781 gaggagctcc gccttcacgt gggggctgag tacggcgccg ccggagagga ggtgtcgcat
      841 cgacgagacg tcgtacggtg ccccggcgtc ggcacggcga gccaactcgt cgagcagggg
      901 gcgggcgaag gagtcaccga cgatcaggag gctggtggcc cggtggcgct cgacggtcga
      961 ccagatgtcg gcggggtcga ggtgttcggg gtggtcctgg atgatgaccg tgccaccggc
     1021 gatccagcag ctgatcgcgt tccaatgcgc tgcgccgtgc atgagcggcg gggccgggag
     1081 cgctcgcagc gacgatcgtt cggcggcggt cacgatctcg tcgatcgact cgtagtcgga
     1141 tccgtcggcc cgacgaacgc ccaaggccga caccacgaag tcggcctggc gccacagcac
     1201 ccccttgggc atgccggtcg tgccgccggt gtagcagagg tagagatcgt cgccggagcg
     1261 ctcgccgagc gggcgagcct cgggggccgc cgtggcgagc gcctcctcgt accaccgggc
     1321 gctgcggccg tcgacgtcgg ccagcagcgg gttgcccgac gagtccgcaa cctgcaggag
     1381 gagcgtgagt gacggaaggt cggggagcac ctcggccagc aggggagcga aggccgagtg
     1441 gaagatgatc gccttcgccc cgctgtcgcc cagcacatag gcgagctcgg ttgcccggta
     1501 gcggtagttg acgttcaccg gcgcgcacac ggccttgtac gcgccgagca tgccttcgag
     1561 gtactcgttg ccgttgtgga ggtagagcgc gacgtggtcc tgggtcgact cccagccgtc
     1621 cgccccggcg aacgggcggg tgcggccgag ccccgcggct ccgaagatcg cagcgaggtg
     1681 gttggtgcgg tcggcgacgt cggcccatgt cagccgacgg tcccggaacg tcaggcactc
     1741 gcgcccgggg taggcaacgc tcaacgcgtc gcagatgtcg gcgatgttca gctccatcga
     1801 gaccgtccca cggccggtca gcatggcagg ctggcccgat ggcagacgaa cccaccgagt
     1861 ccaccgtgct cctcgacacc gtcgagccgg gcatcacccg tatcacgctc aaccgtccgt
     1921 cgtcgctgaa ctcgatgaac cggcagttga tcgacgagct gcacgcggcg ttcgacgtcg
     1981 tcgacgctga ccccgacgtc cgggtggtgc tcctcaccgg cgccggccga gggttctgtg
     2041 ccgggctcga cctgcgtgac cccacgctcc tcatgggccg caacgatccc gagggtcccg
     2101 gggtgccgca gtcgggcatg gcgctgcagc agcggatcgc gtcgctgatc ccgaagatgc
     2161 gccggctccg ggtgccgatc atcgccgccg tcaacgggcc ggccacgggc ggcggtctgg
     2221 cgctcgcact gggctccgac gtccgcatcg cagcggaggc cgctcgtttc ggggtggcgt
     2281 tcattcgcat cggcctcagc gcctgcgaca tcggggtgag ttgggcgctg cctcgactcg
     2341 tcggggcgtc ccgtgcgcac gagctg
//
LOCUS       DAOSOA010000416         2352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_45350_length_2352_cov_3.855464, whole genome shotgun sequence.
ACCESSION   DAOSOA010000416 DAOSOA010000000
VERSION     DAOSOA010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45350_length_2352_cov_3.855464"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2352
                     /locus_tag="PKY13_16600"
     CDS             <1..>2352
                     /locus_tag="PKY13_16600"
                     /inference="COORDINATES: protein motif:HMM:NF023275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HOT81614.1"
                     /translation="FTALPWLVAFTVRSLRQPGWKHPALFALVALASGGINATSLLLV
                     LLAPGLWLVHAIWVEREITFRAAIAPVLRIGLLSVITSLWWVAGLALQGSYGINVLRF
                     TETYRTVSDASTSAEVGRSLGYWFFYGIDTFGPWFKAAATMTQSLPALALSFAVPIVC
                     VACALWVRFRYRLFFVAVALVGLVVSVGSHPLDDPSPYGSLFKAFTDTESGLALRSTP
                     RAIPLLSLALAAFLGAGVAAASRRWPARRLAFAGLALLMVMANLSPLFMGRFIDPFLE
                     RPNDVPDYWKAVGKELDAGDRDTRALEIPGIEFANYRWGASVDPITPGLTDRAYAARE
                     LVPYGSNASANLMNAIDTPLQDRSFDPDSYAAILRMLSVGELIVRNDLEYERFRTPRP
                     RPMERWMERVDGLGVRKAFGPSTPNVATSESPLLDDLELATPDTDADPPAVSVYPVDD
                     PLPVVRTVPDDRAVIIAGDGEGIVTAAEANRLDPDRLVVYSGTVSNDDSTFDGLMATH
                     PELVITDTNRKAGRRWGTTKDNDGYTEMADETAPADPSDNRLDLFDDRGTDVQTVAEQ
                     EGAVRATASGYGNPLSFTPGDRAANAVDADPTTSWKVGAFSDVRGEWLQLATEKPSAP
                     GSLTLTQPSRDVNRFITKVRLTFDGKDPLDVDLGDTSRSSGQRIDLGDRTFSTLRITV
                     VATDRGVLGTYKGVSGVGFSTARLGTTDPTVEVIRPPVDLLDAAGTRSNDLRLSYVFH
                     RRTAPLDSGAVDEETALHRRIDLPVARPFTVEGKLRLSPDIGD"
BASE COUNT          319 a          870 c          770 g          393 t
ORIGIN      
        1 gttcacggcg ttgccgtggc tggtggcgtt cacggtccgg tccctccgcc aaccggggtg
       61 gaagcaccct gctctgttcg ccctggtcgc gctcgcatcg ggtggcatca acgccacgtc
      121 gctgctcctg gtcctcctcg cgcccggcct gtggctcgtg cacgccatct gggtggagcg
      181 cgagatcacg ttccgtgcgg ccattgcgcc ggtcctgcgc atcgggctcc tcagcgtgat
      241 cacgtcgctg tggtgggtcg ccggtctggc gttgcagggc agctacggga tcaacgtgct
      301 gcggttcacc gagacctatc gaacggtgtc ggacgcgtcg acgtcggccg aggtcggccg
      361 ctcgctcggc tactggttct tctacggcat cgacacgttc ggcccgtggt tcaaggccgc
      421 ggcgacgatg acgcagtcac tccccgcgct ggcgctgtcg ttcgccgtgc cgatcgtgtg
      481 cgtggcgtgc gccctgtggg tccggttccg ctaccgcctc ttcttcgtgg cggtcgcact
      541 ggtcgggctg gtggtctcgg tcgggtcgca ccccctcgac gatccgtcac cgtacggctc
      601 actcttcaag gcgttcaccg acacggagtc gggcctcgcc ctgcgctcga ctcctcgggc
      661 catcccgctg ctgtccctcg ccttggcagc cttcctcggt gccggcgtgg ccgcggcgtc
      721 gcgccgttgg ccggcgcgcc gcctggcgtt cgccggcctc gcactcctga tggtcatggc
      781 gaacctgtcg ccgttgttca tgggccgctt catcgacccg ttcctcgagc ggcccaacga
      841 cgtgcccgac tactggaagg ccgtcggcaa ggaactcgac gccggcgacc gcgatacccg
      901 ggcgctcgag atccccggca tcgagttcgc caactaccgg tggggggcct cggttgatcc
      961 catcaccccc gggctcaccg accgggcgta cgccgcccgc gagctcgtgc cctacgggtc
     1021 gaacgcgtcc gccaacctga tgaacgcgat cgacacaccg ttgcaggacc gcagcttcga
     1081 cccggactcg tacgcggcga tcctccggat gttgagcgtg ggcgagctga tcgtccgcaa
     1141 cgacctggag tacgagcggt tccgcacccc tcgcccccgg ccgatggagc ggtggatgga
     1201 acgcgtcgac gggctgggtg tccgcaaggc gttcggtccc tcgaccccca acgtggccac
     1261 gagcgagtcg cccctcctcg acgacctgga gttggcgacc cccgacaccg acgccgatcc
     1321 gccggccgtg tcggtgtatc ccgtcgacga tcccctgccg gtcgttcgga cggtgcccga
     1381 cgatcgagcc gtgatcatcg ccggcgacgg tgaggggatc gtcacggcgg cggaggccaa
     1441 ccggctcgac cccgaccgcc tcgtcgtcta ttccggaacg gtcagcaacg acgactcgac
     1501 gttcgacggt ctcatggcga cccaccccga gctggtgatc accgacacca accgcaaggc
     1561 gggccgccgc tggggtacca cgaaggacaa cgacggctac accgagatgg ccgacgagac
     1621 cgctcctgcc gatccgtccg acaaccggct cgacctcttc gacgaccgcg gcaccgacgt
     1681 gcagaccgtc gccgaacagg agggcgcggt ccgggcgacc gcttcgggct acggcaaccc
     1741 gttgtcgttc acccccgggg accgcgccgc caatgccgtc gacgccgatc cgacgacgtc
     1801 gtggaaggtc ggagcgttca gtgacgtgcg cggcgagtgg ctccagctcg cgaccgagaa
     1861 gccgtccgct ccggggtcgt tgacgctcac ccagccgtcg cgcgacgtca accgcttcat
     1921 cacgaaggtg cgtctcacgt tcgacgggaa ggatccgctc gacgtcgatc tcggcgacac
     1981 gtctcggtcg tcgggccagc gcatcgacct cggcgaccgg accttctcga cgctgcggat
     2041 caccgtggtg gcaacggacc gcggtgtgct cggtacctac aagggcgtca gcggcgtcgg
     2101 cttctcgacg gcgcgtctcg gcacgaccga cccgaccgtc gaggtgatcc gcccgccggt
     2161 cgacctcctc gacgcggccg gcacccggtc gaacgacctt cgcctctcct acgtgttcca
     2221 ccgccgtacc gctccgctcg actccggtgc cgtcgacgag gagaccgcgc tgcaccgccg
     2281 gatcgacctg cccgtggccc gaccgttcac ggtcgagggc aagctccggc tctcgcccga
     2341 catcggcgac ga
//
LOCUS       DAOSOA010000417         2352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_45353_length_2352_cov_3.481933, whole genome shotgun sequence.
ACCESSION   DAOSOA010000417 DAOSOA010000000
VERSION     DAOSOA010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45353_length_2352_cov_3.481933"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PKY13_16605"
     CDS             complement(<1..915)
                     /locus_tag="PKY13_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81615.1"
                     /translation="MRAALRISFRSVRRHPWRSLVVVALVALPVGVLAGALSTMAGND
                     ARQADAIRADLLGGQFVHRFSPSAAEPFGPSSSETVADVRGRFSLPDSARIDRQVTGW
                     YALARTNGEVRVPTWSEADWSTLDTGGLVRLADGRWPTRPGEVAVADDTSAIGSTIDL
                     TLPDLTVTVVGHVQPDDRLPMTHGLIAAPGTFPAVDAPIWSEAGLQTPDVAWVVRGLD
                     RTQLERLGQIEPSYATSSALEQVASVGISTFGFLWCGLVAASALAIGARRRRRELGLL
                     SVNGATPRQLRLSVVADGVVLGGIGAAVG"
     gene            complement(912..1652)
                     /locus_tag="PKY13_16610"
     CDS             complement(912..1652)
                     /locus_tag="PKY13_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT81616.1"
                     /translation="MTQQLALDGLTKSYPDGTTTRVVLDAVDLTVDAGELVAIMGPSG
                     SGKSTLLHLVAGLDRPDSGQIKVGATFLSALSGAELAELRRTAVGYVEQRLNLIAVLS
                     AVENVMLPLELGGMSARAARQQAMAALEAVGLADMADRDAERLSGGEQQRVAIARALV
                     GGRGLLLADEPTAALDSLTGESVMRLLRRRCDDDGIAVVMTTHDASHAAWADRVIFLR
                     DGGVVDEARPGSDSPVSEVSFDDEELAR"
     gene            complement(1649..2194)
                     /locus_tag="PKY13_16615"
     CDS             complement(1649..2194)
                     /locus_tag="PKY13_16615"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT81617.1"
                     /translation="MSIKNGLLSLLLEGPKYGFQLKKEFEEVTGHVWSLNVGQVYTTL
                     GRMVRDGLVTEEDGDDARSGDDGQRSYRLSSAGREAVDAWFTSPRATDQHERDELTIK
                     VAMAVAADRPDIADLLQAQRSEATSSLQALTRRKARLEPDDISQAVVLDAAISRLDAE
                     VRWLDLTTARLAGPKHRGSRT"
BASE COUNT          369 a          844 c          792 g          347 t
ORIGIN      
        1 accgacggcg gccccgatgc cgccgagcac caccccgtcg gcgacgacgc tcagccggag
       61 ctgacggggc gtcgcgccgt tcaccgacag cagccccaac tcccgtcgcc gacgccgcgc
      121 tccgatggcc agcgccgagg cagcgaccag gccgcaccac aggaaaccga acgtcgagat
      181 gcccaccgac gcgacctgct cgagggccga gctggtcgcg tagctcggct cgatctgtcc
      241 gagtcgttcg agctgggtcc gatcgagtcc gcggaccacc cacgcgacgt cgggtgtctg
      301 cagaccggcc tccgaccaga tcggggcgtc gacggcgggg aacgtgcccg gagcggcgat
      361 gagaccgtgc gtcatcggca atcgatcgtc cggctggacg tgaccgacca cggtcacggt
      421 gaggtcgggc agcgtgaggt cgatcgtcga tccgatcgcc gacgtatcgt cggcgaccgc
      481 cacctcaccg gggcgggtcg gccagcgacc gtcggcgagt cgaaccagcc ccccggtatc
      541 gagcgtcgac cagtcggctt cgctccaggt gggcacccgc acctcgccgt tggtccgggc
      601 cagggcgtac caccccgtca cctgtcgatc gatccgggcg ctgtcgggga ggctgaaccg
      661 gccgcggacg tcggcgacgg tctcggagct cgacggcccg aacggttcgg cggcactcgg
      721 agagaatcgg tggacgaact gccctcccag caggtctgca cggatcgcgt ccgcctgccg
      781 ggcgtcgtta ccggccatcg tcgagagcgc accggcgagg acgcccaccg gaagggccac
      841 gagcgcgacg acaacgaggc ttcgccacgg gtggcggcgc accgaccgga acgagatccg
      901 cagggcggcc ctcatcgcgc cagttcctcg tcgtcgaacg acacctccga cacgggcgaa
      961 tccgatcccg gtcgagcttc gtcgaccacc ccaccgtcgc gcaggaagat cacccggtcg
     1021 gcccaggccg cgtgcgacgc gtcgtgggtc gtcatcacca cggcgatccc gtcgtcgtcg
     1081 caccgtcgac ggagcagccg catcacgctc tcaccggtga gcgagtcgag ggccgccgtc
     1141 ggttcgtccg ccagcagcaa ccccctgccg ccgaccagcg cccgggcgat ggcgacgcgc
     1201 tgctgctcgc cgcccgacaa gcgctcggcg tcgcggtcgg ccatgtcggc gagcccgacg
     1261 gcctcgagcg ccgccatggc ctgctgtctc gccgcccgcg ccgacatgcc gccgagctcc
     1321 agggggagca tcacgttctc gaccgcgctc agcacggcga tgaggttcag gcgctgctcg
     1381 acatacccga ccgccgtccg ccgcagctcg gcgagttcgg cgccactcaa ggcggacagg
     1441 aaggtcgcac cgaccttgat ctggccggag tcgggccggt cgagtccggc gacgagatgc
     1501 aggagggtgc tcttgccgga gcccgagggt cccatgatcg ccaccaactc cccggcgtcg
     1561 acagtgaggt cgacggcatc gaggacaacg cgtgtggtgg tgccgtcggg ataggacttc
     1621 gtgagtccat cgagtgcgag ctgttgtgtc atgtcctgct tccacggtgc ttcgggcccg
     1681 cgaggcgggc ggtcgtcagg tcgagccagc ggacttcggc gtcgagccgg ctgatggcgg
     1741 cgtcgaggac gaccgcctgg ctgatgtcat cgggttcgag gcgggccttg cgtcgggtga
     1801 gcgcctggag cgaactcgtc gcctcgctcc gctgcgcctg caacaggtcg gcgatgtcgg
     1861 gacgatcggc ggcaaccgcc atcgcgacct tgatcgtcag ttcgtcgcgc tcgtgttggt
     1921 cggtcgcacg cggtgacgtg aaccaagcgt cgacggcctc gcgccccgcc gaggacagcc
     1981 gataggaccg ctggccgtcg tcaccgcttc gggcgtcatc gccgtcctcc tcggtcacga
     2041 ggccgtcgcg caccatccgc cccaacgtcg tgtacacctg cccgacgttg agcgaccaga
     2101 cgtggccggt gacctcctcg aactccttct tcagctggaa cccgtacttg ggcccctcca
     2161 gaagcagcga gagcaggccg ttcttgatcg acatcgacac ccctctccac gagatctcac
     2221 agctacccac cgagtaacta gttactcagt atgcatactc ggtatggtga tggcaatcgg
     2281 tttcccccca aaacgcaacg aactcccgac gtggaacccg cagtgcgcgg gttctacgtc
     2341 gggagttcgt gg
//
LOCUS       DAOSOA010000418         2311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_46420_length_2311_cov_5.585550, whole genome shotgun sequence.
ACCESSION   DAOSOA010000418 DAOSOA010000000
VERSION     DAOSOA010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46420_length_2311_cov_5.585550"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..1236
                     /locus_tag="PKY13_16620"
     CDS             328..1236
                     /locus_tag="PKY13_16620"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT81618.1"
                     /translation="MRALVTGATGFVGPHLISHLLDEGDDVTATDRTTGLDIADVDGL
                     VDLFETARPEVVYHLAGDSDVGGSWNHPQATFRANAEGTLNVLEAARAAGVDRIVAIG
                     SADVYGKVAEHELPLTEQSELRPTSPYAASKVAADYVALQAFLGYGLGVIRVRAFNHL
                     GPGQSPRFVAPAIATRIAANEREGTDVVPVGNLDARRDLTDVRDVVRAYRLLAELGEP
                     GGVYNVCSGESVRIGELAERLVELATSPMRLVPDPALLRPVDIPVLCGDNTRLREATG
                     WSPKYSIGDTLADLMADCRSRAAANH"
     gene            1290..2270
                     /gene="gmd"
                     /locus_tag="PKY13_16625"
     CDS             1290..2270
                     /gene="gmd"
                     /locus_tag="PKY13_16625"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT81619.1"
                     /translation="MTKRALITGITGQDGSYLAEFLLDQGYEIFGMVRRSSTVTFERI
                     AHLQDRITVVFADLLDQASMIEVLKTYEPDEVYNLAAQSFVPTSFSQPVLTGEVTALG
                     VTRILDAIRLVNPGIRFYQASSSEMFGKVVEVPQRETTPFYPRSPYGVAKLYGHWITI
                     NYRESYDLHASSGILFNHESPRRGIEFVTRKISYGVARIKLGQQQELRLGNLEAQRDW
                     GFAGDYVKAMWAMLQQDEPDDYVVSTGETHSVREFCEIAFDQAGLDWNDFVVVDEKFF
                     RPAEVDLLIGDPAKANKVLGWEAETSFDQLVRMMVDADLDFLQGKLKGIS"
BASE COUNT          393 a          854 c          719 g          345 t
ORIGIN      
        1 gacgtcgtag ccctccacga acggggtgga atcggggctc cagtcgtcgg gatcctcacc
       61 cgcaccggga ccggcaccac gggcggtcga gcgcgtggga acagcagagt tctcggagga
      121 gcggcggcga ggtggcatca ggatggggag cggcacaacg atcggtgcgg cgagcgcaca
      181 tagtagagca ccaccccgac cgggccacgt ggcggcaggc caccgacgac ggcccggcga
      241 ggtcgccacc aggccataga ctgcgccgcc tatgggtggg acactcacaa cgggcacgac
      301 ccgcgaacac gcgtcagtac cggcacgatg agggcactcg tcaccggcgc gaccgggttc
      361 gtcggcccgc acctcatctc gcatctcctc gacgagggcg acgatgtgac cgcgaccgat
      421 cgcacgacgg gcctcgacat cgccgatgtc gacggtctcg tcgacctgtt cgagaccgcc
      481 cgccccgagg tcgtctacca cctcgccgga gacagcgacg tgggcggctc gtggaaccac
      541 ccccaggcca cattccgtgc caacgccgag ggcacgctga acgtgctcga agcggctcgc
      601 gccgccgggg tggaccgcat cgtcgccatc ggcagcgccg acgtctacgg caaggttgcc
      661 gagcacgagc tcccgctcac cgagcagtcc gagctccgtc cgacgagccc ctacgccgcc
      721 tccaaggtcg ccgccgacta cgtcgccctc caggccttcc tcgggtacgg cctcggtgtc
      781 atcagggtcc gggccttcaa tcacctcggc cccggacaga gcccccgctt cgtcgccccg
      841 gccatcgcca cccgcatcgc cgccaacgag cgcgagggca ccgacgtcgt gccggtgggc
      901 aacctcgacg cacggcgcga cctcaccgac gtccgcgacg tcgtccgcgc ctaccgcctg
      961 ctcgccgaac tcggtgaacc cggcggcgtc tacaacgtct gcagcggcga gtcggtgcgg
     1021 atcggcgagt tggccgaacg actcgtcgag ctcgccacct cccccatgcg cctcgtgccc
     1081 gaccctgcac tcctgcggcc cgtcgacatc ccggtgctgt gcggcgacaa cacgcggctc
     1141 cgcgaggcca cgggctggtc gccgaagtac tcgatcggcg acacgctcgc cgatctgatg
     1201 gccgactgtc gcagtcgcgc cgcagccaac cactagtcgc tcgtccgagc cactacagtt
     1261 cctgtcccac ttccttggag tccgaaccca tgaccaaacg cgcactcatc accgggatca
     1321 ccggccagga cggctcgtac ctcgccgagt tcctgcttga tcagggctac gagatcttcg
     1381 gcatggtccg tcgctcgtcg acggtgacct tcgagcgcat cgcacacctg caggaccgca
     1441 tcacggtggt gttcgccgac ctgctggacc aggcctcgat gatcgaggtg ctcaagacct
     1501 acgaacccga cgaggtctac aacctcgcag cgcagtcgtt cgtgcccacg tcgttctccc
     1561 agccggtcct caccggtgag gtcacggcgc tcggcgtcac ccgcatcctc gacgccatcc
     1621 gcctcgtgaa ccccggcatc cgcttctacc aggcgtcgtc gtcggagatg ttcggcaagg
     1681 tcgtcgaggt accgcagcgt gagaccacgc cgttctaccc ccgcagcccc tacggcgtcg
     1741 cgaagctcta cggccactgg atcacgatca actaccgcga gtcctacgac ctgcacgcga
     1801 gctcgggcat cctgttcaac cacgagtcac cgcgccgcgg catcgagttc gtgacccgga
     1861 agatcagcta cggcgtcgct cgcatcaagc tcggccagca gcaggagctt cgcctcggca
     1921 acctcgaggc ccaacgcgac tggggcttcg ccggggatta cgtcaaggcg atgtgggcga
     1981 tgctccagca ggacgagccc gacgactacg tcgtgtccac cggtgagacc cacagcgtcc
     2041 gcgagttctg cgagatcgcg ttcgaccagg ccggcctcga ttggaacgac ttcgtggtcg
     2101 tcgacgagaa gttcttccgc ccggccgagg tcgacctcct catcggtgac ccggcgaagg
     2161 ccaacaaggt cctcggctgg gaggcggaga cgagcttcga ccagctggtt cgcatgatgg
     2221 tcgatgccga cctcgacttc ctgcagggca agctcaaggg catcagctag cgcccgtcga
     2281 gatgatcacc accctcgccg gcggggtcgg c
//
LOCUS       DAOSOA010000419         2292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_46960_length_2292_cov_5.241395, whole genome shotgun sequence.
ACCESSION   DAOSOA010000419 DAOSOA010000000
VERSION     DAOSOA010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46960_length_2292_cov_5.241395"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PKY13_16630"
     CDS             <1..1013
                     /locus_tag="PKY13_16630"
                     /inference="COORDINATES: protein motif:HMM:NF022470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HOT81620.1"
                     /translation="IDRFALTANNITYAGVGDLLGYWDFFPSGADGWGRVPAMGWAEV
                     VESRVDSIDVGGRYYGWFPMAGYVDLAATPTSDGLRDDGAHRAAHAPVYRSYVEQSRD
                     PMLVGTDLGPELGVDHIAEIEERHALLRGLMMTGFLLDEFVAGLPDGPPEQVLVLSAS
                     SKTGIALATRAAARGASVVVGLTSGRNVEFVRSLGCYGQVFAYDDIGSLPTLSSAVVD
                     MAGAAEVVAELHALLDDEIRTSLIVGRSHHDAPPAEVVGGPSPQFFFAPTEVERLLAE
                     WGPAEYRRRAAAALGEFVDGSRDWLTIDRRDGAAEGAQAWADVYAGRIAPSVGVVVSL
                     HG"
     gene            1210..>2292
                     /locus_tag="PKY13_16635"
     CDS             1210..>2292
                     /locus_tag="PKY13_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81621.1"
                     /translation="MGAMDELIDAAALERLRNQLRTASDTAFPAVARAAIEVDGLKLR
                     QRVDLVAAALIDDLPSVYGDAAKIVRSALSDPEFSGWTIWPVGEAMTALALASVIGTD
                     FEDGLAMMADLTPRLSSEFAIRPFLVRDLDRSLAVIEIWTGSDDPHVRRLASEGTRSY
                     LPWAIRVPELLRRPECTVPILDALYRDDDEVVRRSVANHLNDLSRHAPDVVVATAARW
                     TAAPDANTAKVVRHALRTLIKAGLPEALALQGFAPVEVAVANLRVAPDVVELPGEVEF
                     VFDLTNTGDVTADVAVDYVVHFRKKNGSLAAKVFKLTTRSLDPGETVTLSKRHGLRQM
                     TTRVHYAGAHELELQINGQRHGRAVFD"
BASE COUNT          323 a          743 c          841 g          385 t
ORIGIN      
        1 ggatcgaccg attcgcgctg accgccaaca acatcaccta cgccggggtc ggcgacctgc
       61 tggggtactg ggacttcttc ccgagtggcg ccgacggctg gggtcgcgtg ccggcgatgg
      121 gatgggccga ggtggtcgag tcacgtgtcg acagcatcga cgtcggcggt cgctactacg
      181 ggtggttccc gatggccggc tacgtcgatc tcgctgcgac gccgacgtcg gacggccttc
      241 gcgacgacgg ggcccatcgt gccgcgcacg cgccggtgta ccggagctat gtcgagcaat
      301 cccgtgaccc gatgctcgtg gggaccgacc tcggtcccga gttgggtgtc gatcacatcg
      361 ccgagatcga ggagcggcac gcgttgctgc gtgggttgat gatgacgggg ttcttgctcg
      421 acgagttcgt cgctgggctg cctgacggcc cgcccgagca ggtgctcgtc ctgtcggcgt
      481 cgtcgaagac gggcatcgcg ctcgcgaccc gggcggcggc tcggggagcg tcggtcgttg
      541 tcggtctgac gtcgggtcgc aacgtcgagt tcgtgcggtc gctcgggtgc tacggccagg
      601 tgttcgcgta cgacgacatc ggatcgctcc cgaccctgtc gtccgctgtg gtcgacatgg
      661 cgggggccgc cgaggtcgtc gccgaactgc acgcgctgct cgatgacgag atccggacgt
      721 cgttgatcgt cggccggagc catcacgacg cgccgcccgc cgaggtggtc ggcggtccgt
      781 cgccgcagtt cttcttcgct ccgaccgagg tcgagcggtt gctggccgag tgggggccgg
      841 cggagtaccg gcgacgcgcc gccgcggcgc tgggcgagtt cgtcgatggg agtcgtgact
      901 ggctgacgat cgaccggcgc gatggtgcag cggaaggagc gcaggcgtgg gccgacgtgt
      961 acgccggacg gatcgcgccg tcggtcggcg tggtcgtgag cctgcacgga taatgggcct
     1021 ctcgctgtac cgacgcagaa ggtctcgctg agcctcgacg ccgtcatcgt catcgtcatc
     1081 gtcaccggca accccggcga cgggtcgatg ctggcagctc cccttccgaa cctgacggtg
     1141 gacgccgtcg agtagccgga ccccgcgagc ccggccggtc gacaacgcca tccgtgggag
     1201 gctgggtcaa tgggtgcgat ggacgagctg atcgatgccg cggcgttgga gcggctgcgg
     1261 aaccagctgc ggacggcgtc ggacaccgcc ttcccggcag tcgcccgggc cgcgatcgag
     1321 gtcgacggtt tgaagctgcg ccagcgggtc gacctcgttg cggccgcgct gatcgacgat
     1381 ctcccgtcgg tctacggaga tgccgccaag atcgtgcggt cggcgttgtc ggacccggag
     1441 ttctcgggtt ggacgatctg gccggtcggc gaggcgatga cggcgttggc ccttgcctcc
     1501 gtgatcggca ccgatttcga ggacgggctc gcgatgatgg ccgacctcac tcctcgcctc
     1561 tcgagcgagt tcgccatccg cccgttcctc gtgcgcgacc tcgaccgctc gctcgctgtg
     1621 atcgagatct ggacgggtag cgacgatcct cacgtgcggc ggctcgcgag cgagggcacg
     1681 cgctcgtacc tgccgtgggc gatccgagtg ccagaactgc tccgtcgacc ggagtgcacg
     1741 gtgccgatcc tcgacgcgct ctatcgagac gacgacgagg tcgtgcggcg gtcggtggcg
     1801 aaccacctca acgacctcag ccgccacgcc ccggacgtgg tcgtcgccac tgcggcccgg
     1861 tggactgcgg ctccggatgc caacaccgcg aaggtggtgc ggcacgcgct gcgcacgttg
     1921 atcaaggcgg gactgcccga agcgctcgcg ttgcaggggt tcgctccggt cgaggtcgcc
     1981 gtagcgaacc tccgggtggc tcccgacgtc gtcgagctgc ccggggaggt ggagttcgtc
     2041 ttcgacctca cgaacaccgg tgacgtcacc gccgacgtgg cggtcgacta cgtggtgcac
     2101 ttccgaaaga agaacggctc gctcgcggcg aaggtgttca agctcacgac gcgttcgctc
     2161 gatccgggcg agacggtgac gctgtcgaag cgacacggcc tgcgccagat gacgactcgg
     2221 gtgcactacg cgggagcgca cgaactcgag ctgcagatca acgggcaacg gcacgggcgg
     2281 gcggtcttcg ac
//
LOCUS       DAOSOA010000420         2259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_47903_length_2259_cov_4.354809, whole genome shotgun sequence.
ACCESSION   DAOSOA010000420 DAOSOA010000000
VERSION     DAOSOA010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47903_length_2259_cov_4.354809"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1459)
                     /locus_tag="PKY13_16640"
     CDS             complement(230..1459)
                     /locus_tag="PKY13_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT81622.1"
                     /translation="MTDVHDTAAVGAAGGGAAALPQIDLLNGHLYAADPTPTYRWLRH
                     NAPVYRDDINGLWGISRYRDIVDIEKQTRRYSNRNSYRPLPPQEHDDRSMIGQDDPVH
                     QAQRRLVARRFTPRAVASHEDEIRAVVTELIDGALEAGSVDAIDALAAPLPAMMIARY
                     LGFGLDRWKDIQRWSTETIPLGGGPRYHNDTGILAAFEFAVACTELIAEKRANPTDDM
                     VSVWCHTEIDGEPMSDDVIISECLLLVDGGAETTRTVIASTLWDLIEHPDQAQRLRTD
                     PSMIGGSAVEEFIRWVTPILNMARLVTEDHELHGQQLHAGDQLVLMYSSANRDEEVFD
                     DPDTYDVGRAHNNHIAFGFGTHFCLGSSLARLEIKLFFEEFLRRVQSFEITPGTTPNR
                     VPGAFVRGVENLFLDVR"
     gene            complement(1500..>2259)
                     /locus_tag="PKY13_16645"
     CDS             complement(1500..>2259)
                     /locus_tag="PKY13_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT81623.1"
                     /translation="AGANVEIVARDRARGAAAAKRLGAAAGVDPPIVRLADLGDLDAV
                     RALADELREAHDTIDGLVHNAGAMPTRRVVTGDGLESAWASMVAGPHLLTRLLGDLLS
                     RATVVFVTSGGMYTQALPLDDPSYSSGDFKGSIAYSRAKRAQVDLVAEYERRLLPSPN
                     ATVVAVHPGWADTPGVVESLPTFHLVLGPVLRSPAEGVDTVVWAVGSGELLHGGRLYF
                     DRRVRGVSRWPGTATSDADRVALWDLVEQQCGLT"
BASE COUNT          338 a          778 c          811 g          332 t
ORIGIN      
        1 agtcgacgag ccggaagtcc caggtgccgt cgagcgaccg gacgaacggg gacgcgtcac
       61 cggcgcgcgc cgcagccggg tcgtcgtagg ggtcgagcgg aggacgcatc ggcaggcgcc
      121 cccgccccgt cacctccggc gtcgaccagg gatggatacc tccagcggtc accggtccgt
      181 caccgaccgt cgagcgacag gtgcagcctc gacaccaacg tcggagcggt caccggacgt
      241 ccaggaagag gttctcgaca ccccggacga acgcgcccgg cacgcggttg ggcgtcgtgc
      301 ccggcgtgat ctcgaacgac tgcacccggc gcaggaactc ctcgaagaac agcttgatct
      361 cgaggcgggc caggctcgac ccgaggcaga aatgcgtgcc gaacccgaag gcgatgtggt
      421 tgttgtgcgc ccgaccgacg tcgtaggtgt cgggatcgtc gaacacctcc tcgtcgcggt
      481 tggccgagct gtacatcagc accaactggt cgccggcgtg gagctgttgg ccgtgcagct
      541 cgtggtcctc ggtgacgagc cgggccatgt tcaggatcgg ggtcacccag cggatgaact
      601 cctcgaccgc ggacccgccg atcatcgacg ggtcggtccg caggcgttgc gcctggtcgg
      661 ggtgctcgat caggtcccag agcgtggacg cgatgaccgt ccgggtcgtc tcggccccac
      721 cgtcgacgag cagcaggcac tcgctgatga tcacgtcgtc gctcatcggc tcgccgtcga
      781 tctcggtgtg gcaccagacc gacaccatgt cgtcggtggg gttggcacgc ttctccgcga
      841 tcaactcggt gcaggcgacg gcgaactcga acgcggcgag gatgccggtg tcgttgtgat
      901 agcgggggcc gccgccgagc gggatcgtct cggtggacca gcgctggatg tccttccacc
      961 ggtcgaggcc gaaccccagg tagcgggcga tcatcatcgc cggcaacggc gccgccaacg
     1021 cgtcgattgc atcgaccgac ccggcctcga gcgcgccgtc gatcagttcg gtcacgacgg
     1081 cacggatctc gtcctcgtgc gacgcgacgg cgcggggcgt gaaccgacgg gcgacgaggc
     1141 gacgctgcgc ctggtgcacg gggtcgtcct gcccgatcat cgaacggtcg tcgtgctcct
     1201 ggggcgggag cggccggtag gagttgcggt tggagtaccg ccgtgtctgc ttctcgatgt
     1261 cgacgatgtc gcggtagcgg ctgatgcccc agagcccgtt gatgtcgtcg cggtacaccg
     1321 gcgcgttgtg ccggagccag cggtacgtcg gtgtcgggtc ggccgcatac aggtgaccgt
     1381 tgagcaggtc gatctgcggc agcgctgccg cgccaccgcc ggcggcaccg accgctgcgg
     1441 tgtcgtgtac gtccgtcatg caaccccctg gaacgagaac gcgttctacc ggcgtcagac
     1501 tacgtcagac cgcactgttg ctcgacgagg tcccagagcg cgacgcgatc ggcatcggag
     1561 gtcgccgtgc cgggccagcg actgacccca cgcacccggc gatcgaagta gagccggccc
     1621 ccgtggagca gctcgccgct cccgacggcc cacacgacgg tgtccactcc ctccgcaggg
     1681 ctgcgcagca ccggccccag gaccaggtgg aaggtgggca gcgattcgac gacgcccggt
     1741 gtgtcggccc accccgggtg cacggccacc acggtcgcgt tcgggctggg gaggagccgc
     1801 cgctcgtact cggccacgag gtcgacctgg gcgcgcttcg cccgggagta cgcgatggac
     1861 cccttgaagt cgcccgagga gtagctcggg tcgtcgagcg gcagggcctg ggtgtacatc
     1921 ccgccgctcg tcacgaacac caccgtcgcc cgggacagga ggtcgccgag cagacgcgtc
     1981 aggagatggg gaccggccac catcgacgcc cacgccgact cgagcccgtc gccggtgacg
     2041 acccgtcggg tcggcatggc cccggcgttg tgcacgaggc cgtcgatcgt gtcgtgcgcc
     2101 tcccggagtt cgtcggcgag cgcccgcacc gcgtcgaggt cgcccagatc cgcgagccgg
     2161 acgatcggtg ggtcgacgcc ggcggccgca ccgagtcgct tcgctgctgc ggcaccccgg
     2221 gcgcggtcgc gggcgacgat ctcgacgttc gctccggcg
//
LOCUS       DAOSOA010000421         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_48323_length_2243_cov_5.365174, whole genome shotgun sequence.
ACCESSION   DAOSOA010000421 DAOSOA010000000
VERSION     DAOSOA010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48323_length_2243_cov_5.365174"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKY13_16650"
     CDS             <1..721
                     /locus_tag="PKY13_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT81624.1"
                     /translation="MTITAEVLWAAHRRLPVGAEVDASGVLGADRTGPPVRVVVLGDS
                     TLTGPGLTSPDDLWVRQALSALDLDRPVELVSFAVGGSRVGDVRRRLDEALAVDAHAV
                     ILSVGSNDAIHGASTRRFAADYNSLLTELLSRVQVVAVTNVGDLGNIARFPRPLRTIV
                     RSRGRAFCRIVDRVAARHEQAVLLDVTPSNHYFRDRSLFGPDMFHPTSHGHSKWAEVA
                     APGLLLALSRLDTIDPLLRVV"
     gene            complement(786..1499)
                     /locus_tag="PKY13_16655"
     CDS             complement(786..1499)
                     /locus_tag="PKY13_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT81625.1"
                     /translation="MSDLPPSASPATAAPPRRRGGPVPRLSMDLITAAVIELGFENAS
                     LTNVAQHLGAGHGALYRYIGDRDGMMREALERLTASYPWPELADDRREVLWNESKAWW
                     ALCTEYPGFVGALASTPGLPAAMSRRTLLLAKHLNELGVSAADAVMVVDLTTSLVHNI
                     FKWAEQREAIIEQTLAMSVDEAMAQIEGIPDEIVGVLGTVLAGEIWPWYAGQLEIILD
                     GLDARNPPAESPSPEGDAS"
     gene            complement(1523..>2243)
                     /locus_tag="PKY13_16660"
     CDS             complement(1523..>2243)
                     /locus_tag="PKY13_16660"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOT81626.1"
                     /translation="RCEGWTNRDVVVHLDSTNAFWTHSITEGRRGRPTRFLTTFDPVA
                     SPAELVAATEERSGAETVERFAASTDALLALVEQLDSADWTATAESPAGHVSVATVVH
                     HALWDAWVHERDIALPLGFDPAVEDDEVLAALRFVAGLGPAFRIGRGVGRTGRIVIDT
                     TSPESATIVEIGDRVVVRRGSDTAGTDDTDDSDVLRLSGDAVDLLEALSTRSRLDPPV
                     ASEHTWMVSGLHETFDQAPLG"
BASE COUNT          310 a          774 c          808 g          351 t
ORIGIN      
        1 catgacgatc accgccgagg tgttgtgggc ggcgcaccgg cggctccccg tcggagccga
       61 ggtggacgcg tcgggagtgc tgggcgccga ccggaccggt ccgcccgttc gggtcgtggt
      121 gctcggtgac agcacgctga ccggtccggg actcacgagc cccgacgacc tgtgggtgcg
      181 gcaagcactg tcggccctcg atctggaccg gcccgtcgaa ctggtgtcgt tcgcagtcgg
      241 cggctcgcgg gtcggtgacg tccggcgtcg cctcgacgag gccttggcgg tggacgccca
      301 cgccgtcatc ctctcggtcg ggtcgaacga cgcgatccac ggcgcctcaa ctcgccggtt
      361 cgccgccgac tacaactcgc tgctcaccga actcctgtcg cgggtgcagg tggtggcggt
      421 caccaacgtg ggcgacctgg gcaacatcgc ccgcttcccc cgtccactcc gcacgatcgt
      481 ccgctcccgc ggtcgtgcgt tctgccggat cgtcgatcga gtggcggccc gccacgagca
      541 ggccgtcctc ttggacgtga cgccgtcgaa tcactacttc cgagaccgct cgctcttcgg
      601 gcccgacatg ttccacccca cgagccacgg gcattccaag tgggccgagg tcgcggcacc
      661 gggcctcctt ctcgccctca gccgcctcga cacgatcgat ccgctgctcc gcgtcgtgtg
      721 atccgagggc cggcggagcg catgatccaa cgagcgccgc tcgcgtcccg accatcgagc
      781 agcggctagg aagcgtcgcc ttcggggctc ggcgattcgg caggggggtt ccgggcgtcg
      841 agtccgtcga ggatgatctc gagctggccg gcgtaccagg gccagatctc accggcgagc
      901 accgtgccca gcaccccgac gatctcgtcg gggatgcctt cgatctgggc catggcctcg
      961 tcgaccgaca tggcgagggt ctgttcgatg atcgcctccc gctgctccgc ccacttgaag
     1021 atgttgtgga cgaggctggt ggtgagatcg acgaccatca ccgcgtcggc agcgctcacg
     1081 ccgagctcgt tgaggtgctt ggcgaggagc aacgtccggc ggctcatggc cgccggcagg
     1141 ccgggggtcg aggccagcgc accgacgaac ccggggtact cggtgcacag cgcccaccac
     1201 gccttcgact cgttccacaa cacctcacga cggtcgtcgg ccagttcggg ccacggatag
     1261 gaggcggtca ggcgttcgag cgcctcgcgc atcatgccgt cgcggtcgcc gatgtagcgg
     1321 tacagggcgc cgtggccggc gccgaggtgc tgggcgacgt tggtgagtga ggcgttctcg
     1381 aagccgagct cgatcaccgc tgcggtgatc aggtccatgc tgagacgtgg gacggggccg
     1441 ccccggcgtc gaggcggtgc cgccgtcgcc ggactggccg acggagggag gtcgctcact
     1501 ccccgtacct tacgcgcgtg ggtcagccga ggggcgcctg atcgaacgtc tcgtgaagtc
     1561 ccgacaccat ccaggtgtgt tcggacgcga cgggagggtc caggcgcgac cgcgtgctga
     1621 gcgcctcgag caggtcgacg gcgtcgcccg agagccggag aacgtcgctg tcgtcggtgt
     1681 catcggtgcc agccgtgtcg ctcccccgcc gcaccacgac ccgatcgccg atctcgacga
     1741 tcgtcgccga ctccggtgac gtggtgtcga tgacgatccg gcccgttcgg ccgacgccgc
     1801 ggccgatcct gaaggcgggg ccgaggcctg cgacgaagcg gagggcggcc agcacctcgt
     1861 cgtcctcgac ggcggggtcg aacccgagcg ggagggcgat gtcgcgttcg tggacccagg
     1921 cgtcccagag cgcgtgatgg acgacggtcg cgacggacac gtggcccgcc ggcgattcgg
     1981 cggtggcggt ccagtcggcg gagtcgagct gttcgaccag cgcgagcagc gcgtcggtgg
     2041 acgccgcgaa ccgctcgacg gtctcggcgc cggagcgctc ctccgtcgct gcgaccagct
     2101 cggcgggcga cgccaccggg tcgaacgtgg tgagaaaccg tgtcggtcgc ccgcggcggc
     2161 cttcggtgat cgagtgggtc cagaacgcgt tggtggagtc gaggtgcacc acgacgtcgc
     2221 ggttcgtcca tccttcgcag cga
//
LOCUS       DAOSOA010000422         2233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_48622_length_2233_cov_3.004591, whole genome shotgun sequence.
ACCESSION   DAOSOA010000422 DAOSOA010000000
VERSION     DAOSOA010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48622_length_2233_cov_3.004591"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKY13_16665"
     CDS             <1..439
                     /locus_tag="PKY13_16665"
                     /inference="COORDINATES: protein motif:HMM:NF021996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HOT81627.1"
                     /translation="PTFASGAAAAPTTTAASTATTAPPAEPASTPTTASPTTTAATPA
                     VREVARGTFTEVDHPGVGTAVILSDGTQTFVRFEDDFATDNGPDLFAVVNQGDRLIEL
                     APLKGNKGSQNYELPADLDPATVTGVGVWCKRFDATFTTAEFD"
     gene            complement(452..877)
                     /locus_tag="PKY13_16670"
     CDS             complement(452..877)
                     /locus_tag="PKY13_16670"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT81628.1"
                     /translation="MATNADRWAVDTSVAVAAVDSSHAAHGSCAAIVRSRRPALAGHA
                     AFETYSVLTRMPGDLQVDASSARDILGRTFPDVYWVAPDRLADLWVRLAVLGITGGSV
                     YDALVGEAARTNGSRLISRDLRARRTYDLLGVDCEYLAM"
     gene            complement(861..1106)
                     /locus_tag="PKY13_16675"
     CDS             complement(861..1106)
                     /locus_tag="PKY13_16675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT81629.1"
                     /translation="MMVTIDRAGRVVIPKELRDRFSIGPETELDVTADGDGIRLTPAR
                     RPGRAVIDDDGWPVIERLAGIVVTDADVSRWRDGDQR"
     gene            1324..1989
                     /locus_tag="PKY13_16680"
     CDS             1324..1989
                     /locus_tag="PKY13_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81630.1"
                     /translation="MNVLRRTEQRGRVEADEAGADAAIPHLHETNVSTAPSRAEYEIS
                     TPIVASLNEILPASFLSAYPSHSLIARSIDCSVNTTVTTAVIALRIVTTVGPTAAEHH
                     AATGDRRHHLAQGFALFGLVSGVGVIVHVAVFQFLQDNGWGNGVFSVGPTRLIHDSIG
                     FVVALISNDFLNVTYIWRRRPEEEPGMHPPPSRGRSQTTSISATIRLIRSTKSRATSM
                     GGP"
     gene            complement(1911..>2233)
                     /locus_tag="PKY13_16685"
     CDS             complement(1911..>2233)
                     /locus_tag="PKY13_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81631.1"
                     /translation="CGFDAIITIDAVSGESLPFLMFFGAAEADSTGGIMWTLEAHRDL
                     FGPEATFGPHELPIGDDEFGDLYVMDAAVGAITFRPCYGPPMDVARDFVDLISRMVAE
                     IEVV"
BASE COUNT          411 a          789 c          711 g          322 t
ORIGIN      
        1 accgacgttc gcttcgggcg cggccgccgc gccgaccacg accgccgcgt caacggcgac
       61 gaccgcgccg cctgccgaac cggcgtcgac gccgaccacg gcatcgccga ccacgacagc
      121 ggcgacacca gccgttcgcg aggtggcacg gggcacgttc accgaggtcg atcaccccgg
      181 cgtcggcacc gcggtgatcc tgagcgacgg cacgcagacg ttcgtccggt tcgaggacga
      241 cttcgcgacc gacaacggcc ccgacctctt cgccgtcgtc aaccagggcg accgcctcat
      301 cgaactcgca ccgctcaagg gcaacaaggg ttcgcagaac tacgagttgc ccgccgacct
      361 cgacccggcg accgtgaccg gcgtcggcgt gtggtgcaaa cggttcgacg ccacgttcac
      421 caccgcggag ttcgactgac cggacgttcc gctacatcgc caggtactcg caatccacgc
      481 ccagaaggtc gtaggtacgt cgggcgcgaa ggtcccgaga gatcagccga ctcccgttcg
      541 tccgggccgc ctcgccgacg agggcgtcat agaccgagcc accggtgatc cccagcacgg
      601 ccaaacgaac ccagagatcc gccaaccgat cgggcgccac ccagtacaca tcgggaaagg
      661 tgcggccgag gatgtcacgg gccgaggacg catcgacctg caagtctccg ggcattcgtg
      721 tgagaaccga ataggtctcg aaggcagcgt ggccggccaa cgcaggtcgt ctcgatcgca
      781 cgatcgcagc gcacgaacca tgagcggcgt gactggagtc gacggcagcg accgcgacgc
      841 tcgtgtcaac cgcccagcga tcagcgttgg tcgccatcgc gccatcgcga cacgtcagca
      901 tcggtcacga cgatgccggc cagccgttcg atcacgggcc acccatcgtc gtcgatcacc
      961 gctcgacccg gcctgcgggc gggcgtcagg cgaatgccgt cgccgtcggc cgtcacatca
     1021 agctcggtct cgggtccaat cgagaagcgg tcccggagct ccttcgggat gacaactctc
     1081 ccggcccgat cgatcgttac catcatggat gccatggtac cacccggcgc agtggaccga
     1141 ccgagaaggg ctcgatccct gccgatgccc gcgtcctggt cggcgaaacg acgagtcatc
     1201 gagcgtggca tcccagcagg aggccggcac cggccggtcc gcccagagtg gcaggaggcg
     1261 ccgcaacgcc gcagcgtgcg actcgagcac ctcgggaagc tgcttcgagc tacgggcggt
     1321 ggagtgaacg tccttcgcag gaccgagcag cgcggccgtg tagaggcaga cgaggccggc
     1381 gccgacgctg cgatccccca cctgcacgaa accaacgtgt cgacggcccc ttctcgtgcg
     1441 gagtacgaga tctcgacgcc gatcgtggcg tcgctgaacg agatcttgcc cgcctcgttc
     1501 ttgtcggcat acccgtcgca ctcgctgatc gcccggtcga tcgactgcag cgtcaacacg
     1561 accgtcacca cagcggtgat cgctttgagg atcgtgacga cggtggggcc gacggccgcg
     1621 gagcaccacg cagcgaccgg agaccgacgc caccacctgg cccaaggatt cgccctcttc
     1681 ggactggtgt cgggcgtggg tgtgatcgtg cacgtcgccg tgttccagtt cctccaggac
     1741 aacggctggg gcaacggtgt gttcagtgtc gggccgaccc ggctgatcca cgacagcatc
     1801 ggcttcgtcg tcgcgctcat cagcaacgac ttcctcaacg tcacctacat ctggcggcgc
     1861 cggcccgagg aagagccggg gatgcacccc ccgccctctc gtgggcgatc tcaaacgacc
     1921 tcgatctccg ccaccatccg actgatcagg tcgacgaagt cccgcgcgac atccatgggc
     1981 ggcccgtagc acgggcggaa ggtgatcgct ccgaccgccg catccatcac gtagaggtca
     2041 ccgaactcgt catcaccgat gggaagttcg tgggggccga acgttgcctc ggggccgaac
     2101 aggtcgcgat gtgcctccag ggtccacatg atgccgccag tgctgtctgc ctcagcggcg
     2161 ccgaagaaca tgaggaacgg caagctctca ccggagacgg catcgatggt gatgatcgcg
     2221 tcgaacccgc agc
//
LOCUS       DAOSOA010000423         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_48746_length_2228_cov_4.634146, whole genome shotgun sequence.
ACCESSION   DAOSOA010000423 DAOSOA010000000
VERSION     DAOSOA010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48746_length_2228_cov_4.634146"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PKY13_16690"
     CDS             complement(<1..197)
                     /locus_tag="PKY13_16690"
                     /inference="COORDINATES: protein motif:HMM:NF020332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HOT81632.1"
                     /translation="MLDLVLSIAKLRVGQEAYTLSGVAQSLDDYYTGRGEADGVWLGV
                     GAERLGLDGPVAADDLRAVLAG"
     gene            complement(333..815)
                     /locus_tag="PKY13_16695"
     CDS             complement(333..815)
                     /locus_tag="PKY13_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81633.1"
                     /translation="MNDSIREPAGYDWAHYGRHRLRAPDAVIVELPVPVEASSQVWVA
                     TIWPDDRIPGGWARALWEPTGRGWRLPLQVAAGDVIEFGADTPARAVRWFGIMDAYDP
                     DRWATFQGPYPTPGDAWSDAQRLLALERFQPALRSEPDPPPRHRERTGRHARHHRRPR
                     "
     gene            complement(881..1588)
                     /locus_tag="PKY13_16700"
     CDS             complement(881..1588)
                     /locus_tag="PKY13_16700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOT81634.1"
                     /translation="MHDDVRSANDVFVRAVSALSDAQLRAPSRLPNWTRGHVAAHVAL
                     NAEGFVEVASALRSGQRAFMYPSGLAERDQAIDDLALASSEELTARVRDAGLAFMEAW
                     DPLPPIGDCATAPGYPTFSPSTVLQRRLRELQVHLVDLALHDFGVECWSASFVDSDLG
                     LQWATVEARTNQPVAAVDEFGVLWRAGRVLHEHEPVHVSRRLLLAWVLDRGQIEGLPL
                     LQPWSNRSKWEHLPTTA"
     gene            complement(1687..1971)
                     /locus_tag="PKY13_16705"
     CDS             complement(1687..1971)
                     /locus_tag="PKY13_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81635.1"
                     /translation="MNRPPYRRCDEIVAYTYRAALYCPACLIETMIADGIAAPAARDM
                     PTDDVLEQCAGAMGIDQDDDTSYDTDEFPKPVLLDWVTDADVCARCDEPL"
     gene            complement(1968..>2228)
                     /locus_tag="PKY13_16710"
     CDS             complement(1968..>2228)
                     /locus_tag="PKY13_16710"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HOT81636.1"
                     /translation="DDAYAAEELVAELGAAMWCAQAGLSAVTRTDHAAYLAGWLRILR
                     ADARALVTVASRAQAAVDWLNAHTGHTTSDTAELVEREEVAA"
BASE COUNT          338 a          722 c          829 g          339 t
ORIGIN      
        1 ccggcgagca ccgcacggag gtcgtcggca gcgaccgggc cgtcgagccc gaggcgttcg
       61 gcgccgacac cgagccagac gccgtcggct tcgccgcggc cggtgtagta gtcgtccagc
      121 gactgggcga ccccggaaag cgtgtatgcc tcttggccca cacggagctt ggcgatgctc
      181 aacacgaggt cgagcatccg gaaccgaact cccggagtta ctcccgatca aactcccgaa
      241 acccgtgcac aacgcccggt gatacctctg tgaactgaac gatgctgact cccggctcgg
      301 ctgcccggtc gacggcgggc tcgtcggtgg ggctatcggg ggcgacggtg gtggcgggcg
      361 tgtcttccgg tccgctcccg atgccgtggc ggcgggtcgg gttcgcttcg cagggctggt
      421 tggaagcgtt cgagcgcgag cagacgctgc gcgtcactcc acgcgtcgcc cggggttggg
      481 taggggcctt ggaacgtcgc ccaccggtcg gggtcgtagg cgtccatgat cccgaaccac
      541 cgcactgcgc gcgctggggt gtcggcgccg aactcgatca cgtcgccagc ggcgacttgc
      601 agcgggaggc gccagccgcg tccggtcggt tcccacaacg cccgggccca gccgccgggg
      661 atgcggtcgt cgggccagat cgtggcgacc cacacctgcg agctggcctc gaccgggacg
      721 gggagctcga cgatgacggc gtcaggggcg cggagccggt gccggccgta gtgggcccag
      781 tcgtagccgg ccggttctcg gatcgagtcg ttcatgtgcg cctccacggt tcggatggcg
      841 agcgacggta gaagccggcg attcccgagt tgcctgtagc tcaggccgtt gtcgggaggt
      901 gctcccactt cgagcggttc gaccagggtt ggagcagcgg caggccctcg atctggcctc
      961 gatccagtac ccaggccagc aggagccggc gagacacgtg taccggctcg tgttcgtgga
     1021 ggacgcgccc tgcgcgccac aagacgccga actcgtccac ggccgcgacg ggctggttag
     1081 ttcgggcctc gacggtggcc cactgcagcc caaggtccga gtccacgaac gacgccgacc
     1141 agcattcgac cccgaagtca tgcagggcaa ggtcgacgag gtgaacctgc agctcgcgca
     1201 ggcggcgttg aagcacggtg gagggtgaga acgtcgggta cccgggagca gtggcgcagt
     1261 cgccgatcgg cggcagaggg tcccacgcct ccatgaacgc aagacccgcg tcgcgaactc
     1321 gtgctgtcag ttcctcgctg gatgcgagcg cgagatcgtc gatggcctga tcgcgctcgg
     1381 cgaggccgct gggatacatg aaagcccgct ggcccgaccg cagcgcactc gcgacttcga
     1441 cgaaaccttc ggcgttcaaa gccacgtgtg cggccacgtg gccgcgggtc cagttgggca
     1501 agcggctcgg cgctcgaagt tgcgcatcgg acaacgcgct cacggcacgg acgaagacgt
     1561 cgttggcgga tcgaacatcg tcgtgcacat gaacgacgat aggcggcacc cgcccgcagg
     1621 gcggtcacgg cacacccgcc agcaacaccg accgccgcat cctgcgacgg ccggccgttg
     1681 cggcagtcag agcggttcgt cgcagcgggc gcacacgtcg gcgtcggtaa cccaatcgag
     1741 cagcacgggt ttggggaatt cgtcggtgtc gtagctggtg tcgtcgtctt ggtcgatgcc
     1801 catcgcgccg gcgcattgct cgagcacgtc atcggttggc atgtcacggg cggcgggcgc
     1861 ggcgatcccg tcggcgatca tggtttcgat gaggcaggcc gggcagtaga gggcggcccg
     1921 gtaggtgtag gcgacgatct cgtcgcagcg gcggtacggc gggcggttca cgccgcgacc
     1981 tcctcacgct cgaccagctc ggcggtgtcg ctggtggtgt ggccggtgtg ggcgttgagc
     2041 cagtcgacgg cggcttgggc tcgactggcg acggtgacca gggcccgggc gtcggcgcgc
     2101 aggatgcgga gccatccggc gaggtaggcg gcgtgatcgg ttcgggtgac ggcggacagc
     2161 ccggcctggg cgcaccacat ggcggcgccg agctcggcga cgagttcttc ggcggcgtag
     2221 gcgtcgtc
//
LOCUS       DAOSOA010000424         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_49586_length_2201_cov_5.042404, whole genome shotgun sequence.
ACCESSION   DAOSOA010000424 DAOSOA010000000
VERSION     DAOSOA010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49586_length_2201_cov_5.042404"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKY13_16715"
     CDS             <1..289
                     /locus_tag="PKY13_16715"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT81637.1"
                     /translation="IRKLQLDYIKLGRQLTSARGSTAHDDAITNSVVSLARSLGFTTI
                     AQGIEDPASLERARTFGCDLAQGFYFGVAEPADAIDLQIATGRSTSRAVFR"
     gene            complement(358..1272)
                     /locus_tag="PKY13_16720"
     CDS             complement(358..1272)
                     /locus_tag="PKY13_16720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT81638.1"
                     /translation="MPVPSNTVAPVTVAPVTVVIVHWNQPDAAAATIGEFRVQTAVAV
                     AGLRCIVVDNDSRPEQRARLHELLGHAPDVEIVDRPDNGGFGGGANTGLQRWLDDVDG
                     SEWAFLCPHDVSMAPDCIELLLAATAAEPMAGLACADVGDGHIPYIDPYFGGITMPGS
                     PEPGWQPADYPHGTLMALRRECVAEVGLFDEAFFAYCEEADLALRARAAGWTCGLVRG
                     ARVQNVHLGSSVALVDYLQQRNTLRLVRTMSGRYHVAIRLLISLGQVVSGVLRPATAP
                     LVFHPRARLLGMRDFLLGRFGPPPADVF"
     gene            complement(1351..1938)
                     /locus_tag="PKY13_16725"
     CDS             complement(1351..1938)
                     /locus_tag="PKY13_16725"
                     /inference="COORDINATES: protein motif:HMM:NF015018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOT81639.1"
                     /translation="MDSRPSDVPSPLKVPMPVKIVVAGGFGVGKTTFVGSISEIDPLR
                     TEAAMTGRSVGVDVVNDQTAKTSTTVAMDFGRITLGNDDLILYLFGTPGQERFHFMWD
                     ELVKGAIGAVILVDTTRLADSFAAIDYFEARGLPFVVSVNCFNGVVTHNTDDVRAALA
                     LSESVPLFLTDARERTSTRDALLTLVTHALSLARG"
     gene            complement(1919..>2201)
                     /locus_tag="PKY13_16730"
     CDS             complement(1919..>2201)
                     /locus_tag="PKY13_16730"
                     /inference="COORDINATES: protein motif:HMM:NF017170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HOT81640.1"
                     /translation="LPLETLVRTNERGRSVRHEVGAEERRILDLASEPMSVAEISAHL
                     GVVLGVARVLVGDLLATGRLDRSAHPSATASPGSDVSLLERVLDGLKAL"
BASE COUNT          372 a          750 c          774 g          305 t
ORIGIN      
        1 catccgcaag ctgcagctcg actacatcaa gctcggccgc cagctgacca gcgcccgcgg
       61 ctcgacggcc cacgacgatg ccatcaccaa cagcgtcgtg tcgctcgccc ggtccctcgg
      121 cttcaccacg atcgcccagg gcatcgagga cccggcgtcg ctcgaacgtg cccgcacgtt
      181 cgggtgcgac ctcgcccagg gcttctactt cggagtggcc gagcccgccg acgcgatcga
      241 cctgcagatc gcgaccggac gctccacgag ccgcgccgtc ttccgctgac gctccagttt
      301 cgcgggtact tgcgttcacc ccctgggcac aactacccgc agaagctgag gggcgggtca
      361 gaacacgtcg gccgggggtg ggccgaagcg gcccaacagg aagtcgcgca tccccagcag
      421 gcgcgcccgg ggatggaaca ccagcggggc cgtcgccggc cggagcaccc ccgaaacgac
      481 ctgaccgagg ctgatgagca gccggatggc cacgtggtac cggcctgaca tcgtgcggac
      541 caggcgcagc gtgttgcgct gctgcaggta gtcgacgagg gccacgctcg accccaggtg
      601 cacgttctgc acgcgggctc cccgcacgag cccgcacgtc cagccggcgg cacgagcccg
      661 cagggcgagg tcggcctcct cgcagtacgc gaagaaggcc tcgtcgaaca gcccgacctc
      721 ggcgacgcac tcacgccgga gggccatgag cgtgccgtgg gggtagtcgg cgggttgcca
      781 gccgggttcg ggtgaccccg gcatcgtgat gccgccgaag taggggtcga tgtaggggat
      841 gtggccgtcg ccgacgtcgg cgcaggcgag gccggccatg ggttccgccg cagtggcagc
      901 gaggaggagc tcgatgcagt cgggtgccat cgacacgtcg tgagggcaca ggaaggccca
      961 ctccgagccg tcgacgtcgt cgagccagcg ctgtaggccg gtgttcgctc ccccgccgaa
     1021 cccgccgttg tcgggccgat cgacgatctc gacatccggc gcgtggccga gtagctcgtg
     1081 cagacgggca cgctgctctg gccgtgagtc gttgtcgacg acgatgcacc gcagtcctgc
     1141 caccgcgacc gctgtctgaa cccggaactc gccgatcgtc gccgcggcag cgtcgggttg
     1201 gttccagtgg acgatcacga cggtgacggg agcgacggtg accggagcga cggtgttcga
     1261 ggggacaggc acgaccggtc atcctcgcgc agctgcaggc cgtgtcgacc cgaacaaccc
     1321 gatgacctgg ttccccacga ccaccggggg ttagccgcga gcgagggaca gcgcgtgggt
     1381 gacgagcgtg aggagcgcgt cgcgagtcga cgtccgctcc cgggcatcgg tcaggaacag
     1441 cggcaccgac tcgctgagcg ccagcgcggc ccggacgtcg tcggtgttgt gcgtgacgac
     1501 cccgttgaag cagttcacgc tcacgacgaa gggcaggcct cgggcctcga agtagtcgat
     1561 ggcagcgaac gagtcggcga gtcgcgtggt gtcgacgagg atgacagcgc cgatcgcgcc
     1621 cttcaccagc tcgtcccaca tgaagtggaa ccgctcctgg ccgggcgtgc cgaacaggta
     1681 gaggatcagg tcgtcgttgc cgagcgtgat gcgcccgaag tccatggcaa ccgtcgtgga
     1741 ggtcttggcg gtctggtcgt tgacgacatc gacgccgacg ctgcggccgg tcatcgcggc
     1801 ctcggttcgc agagggtcga tctcggagat cgacccgacg aacgtggtct tgcccacacc
     1861 gaacccgccg gcgacgacga tcttcaccgg catcgggacc ttgagcgggc tcggcacgtc
     1921 agagggcctt gagtccatcg agcacccttt ccaagagaga gacgtcggat ccgggggacg
     1981 ctgtcgccga cggatgggcg ctgcgatcga gacgacccgt cgcgaggaga tcgccgacga
     2041 gcacccgggc cacacccagc accacgccga ggtgggccga gatctcggca accgacatcg
     2101 gctcggaggc gaggtcgaga atccggcgct cctcggcacc gacctcatgg cgcaccgaac
     2161 gaccgcgctc gttggtacgc accagcgtct cgagcgggag a
//
LOCUS       DAOSOA010000425         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_49774_length_2195_cov_6.881776, whole genome shotgun sequence.
ACCESSION   DAOSOA010000425 DAOSOA010000000
VERSION     DAOSOA010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49774_length_2195_cov_6.881776"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PKY13_16735"
     CDS             complement(<1..650)
                     /locus_tag="PKY13_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81641.1"
                     /translation="MGLRRDRVRLAAWAFGLSIFPVASYTSYESLFPSEASRRALVAN
                     FGLTPAFRLLLGPARELAQPGGFTAWRSGLFSGVFVAVMAIFTVVRHTRAEEDAGRAD
                     LLAAGVVGRRAMLASGWTLAVGTAAVAGVGAAGFLLAVGAPVAGSVAMGAAVLATGAV
                     FASIAAVAAQLGSFAHTANGLASAVLGVAYVVRGWGASYDGVSWVSWWSPLGWGQQL"
     gene            complement(686..1603)
                     /locus_tag="PKY13_16740"
     CDS             complement(686..1603)
                     /locus_tag="PKY13_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT81642.1"
                     /translation="MPAAISVRGLTKTFGHTLALDDLDLTVTTGEVHGFLGPNGAGKS
                     TAIRVLLGLIRADRGEVSLLGGDPWRDAAELHRRLGYVPGDVSLWPQLSGGEAIDLFG
                     RLRGGLDPVRRSELIERFELDPTKRGRSYSKGNRQKVALVAALAADVELLILDEPTAG
                     LDPLMEAVFQDVIREEKRRGRTVLLSSHILSEVEALCDRVSIVRNGHVVESGSLAELR
                     HLSRTVVRAEVARPVDGLADRPDVHHLRAEGRRVQFSVDNGSLDDVLPLLVAAGIESL
                     TCQPPTLEELFLRQYDGTGAGAGAGTESQ"
     gene            complement(1617..1862)
                     /locus_tag="PKY13_16745"
     CDS             complement(1617..1862)
                     /locus_tag="PKY13_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81643.1"
                     /translation="MPLGNFYKRGCVEGMEFEVVLLNEDEQASCKDQLRALGVKVRDE
                     AMLGHFTIHLDDPLQWGEVGSIIYGEIFADSPDRHHV"
BASE COUNT          385 a          826 c          702 g          282 t
ORIGIN      
        1 agctgctgtc cccaaccgag cggcgaccac cacgacaccc acgacacccc gtcgtacgag
       61 gcgccccacc cccgcaccac gtacgcgaca ccgagcacgg ccgacgccag gccgttcgcc
      121 gtgtgggcga acgatcccaa ctgcgcggcg acggcggcga tcgacgcgaa cacggcaccg
      181 gtcgccagca ccgcggctcc catcgcgacc gatccggcaa ccggagcgcc caccgccagc
      241 aggaacccgg ccgcccccac gccggcgacc gccgccgtgc cgaccgccaa cgtccagccc
      301 gacgccagca tcgcccgacg cccgacgaca ccggcagcga gaagatcggc acgtccggcg
      361 tcctcctcgg cccgcgtgtg tcggaccacg gtgaagatgg ccatgacggc cacgaacact
      421 ccggagaaca gtccggaccg ccacgccgtg aaccctccgg gctgcgccaa ctcacgggcc
      481 ggcccgagca acaggcggaa cgccggggtc agcccgaagt tcgccaccag tgctcgtcgg
      541 gacgcctcgg acgggaagag gctctcgtag ctcgtgtaac tcgccaccgg gaagatcgag
      601 aggccgaacg cccacgccgc cagacgcacc cggtcacggc ggagacccaa ccggacgatc
      661 ggaccgtacc cgacgaacga tcccctcact gcgactcagt ccccgctccc gctcccgctc
      721 cggtgccgtc gtactggcgc aggaacagct cctcgagcgt cgggggctgg caggtcaacg
      781 actcgatccc cgccgcgacc aagagcggga gcacgtcgtc gagcgacccg ttgtcgacgc
      841 tgaactgcac ccgccgcccc tcggcccgca ggtggtggac atccggccgg tcggcgagcc
      901 cgtcgaccgg ccgggcgacc tcggcccgca ccaccgttcg ggacaggtga cggagctcgg
      961 cgaggcttcc gctctcgacg acatggccgt tgcgcacgat gctcacacgg tcacacagcg
     1021 cctccacctc gctcaggatg tggctcgaca gcaacacggt gcgtccccgg cgcttctcct
     1081 cgcggatcac gtcctggaac acggcctcca tcagcgggtc gagcccggcg gtcggctcgt
     1141 cgagaatcag cagttcgaca tcggcggcga gcgcggcgac cagcgcgacc ttctgccggt
     1201 tgcccttgga gtagctgcga ccccgcttgg tggggtcgag ctcgaaccgt tcgatcagct
     1261 ccgaacgccg cacgggatcg aggccgcccc ggagccgccc gaacaggtcg atggcttcgc
     1321 cgcccgacaa ctgcggccac aggctcacgt cgcccggcac gtacccgagg cggcggtgga
     1381 gttcggcggc gtcgcgccac gggtcgccgc cgagcagtga cacctcgccc cggtcggccc
     1441 ggatgagacc gagcagcacc cggatggcgg tcgactttcc ggcaccgttc ggaccgagga
     1501 acccgtggac ctcaccggtc gtgaccgtca gatcgaggtc gtcgagggcg agggtgtggc
     1561 cgaacgtctt ggtcaggcca cggaccgaga tggcagcggg cacgccgctc agccgctcag
     1621 acgtgatggc ggtcgggcga gtcggcgaag atctcgccgt agatgatcga accgacctca
     1681 ccccactgca gcggatcgtc gaggtggatc gtgaagtgcc cgagcatcgc ctcgtcgcgc
     1741 accttcacac cgagggctcg gagctggtcc ttgcacgagg cctgctcgtc ctcgttgagg
     1801 agcacaacct cgaactccat cccctcgaca cagcctcgct tgtagaagtt tccgagtggc
     1861 atgagcggcc tgctgttctg acgtccgacc cacggcgcag gtagggactg ctgatgagag
     1921 cggggaaaag tctgacacaa ccgcccattc ggcgggcgac attgaccaag atatcgggga
     1981 gaccggccgg tcccacccac gtccgatgtc accgccgccc ccgctcgagg caagcatcgg
     2041 gacaacgtgc acccgtttcg caccgaaagg ccgtccttgc cggagtccga tccacccgac
     2101 agcgtcgacg tgacacttac cgaccacacc gtcatcgagg cgatggcccg cgccgtcatc
     2161 gtcgtcgatc ccgatgggcg catcgtgcgc tggaa
//
LOCUS       DAOSOA010000426         2173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_50487_length_2173_cov_5.650614, whole genome shotgun sequence.
ACCESSION   DAOSOA010000426 DAOSOA010000000
VERSION     DAOSOA010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50487_length_2173_cov_5.650614"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKY13_16750"
     CDS             <1..281
                     /locus_tag="PKY13_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81644.1"
                     /translation="HGTAIGVSIVEPGFIRDAGMFAESGIALPKGVRTKSPADVARGV
                     IGAIEGDKGEVFVAPLELRLSATLATVAPALSSRIQRAIDTAGMKGTK"
     gene            290..1816
                     /locus_tag="PKY13_16755"
     CDS             290..1816
                     /locus_tag="PKY13_16755"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT81645.1"
                     /translation="MNRSVLATLLVGALVVGGCSSTDDAADDGPTSTRAESTTTSVPA
                     PPELPALRAIRGDQPRIVDDQERQVILRGANLNSLGDYHQGDPDAVPVVPVTDADWAE
                     MAAHGFNVVRLLVSWSKLEPERGTFDEEYLATVADAVDAAAAHGIYSVVDMHQDAWGK
                     YIASPAGETCPDGGDPAIGWDGAPEWATLTDGASTCTFGSRESSRAVINAWDSFYANR
                     DGIMDELVRTWAFVVGGLGDRPSIAGYDLLNEPNVGSGETPATDQLGRFYDQAITAIR
                     AAEEPLGVDRIVFFEFTVAGQPVPNDFTADDNIVFAPHHYGESIGAIPIEGLFDYLAS
                     LAAGYRTALWVGEYGFFEDTDAAGAKLERYAAKEDALVTAGDAWWQWRQACGDPHSVG
                     RPGGTSDPVQIHLQTNRCPGDVNGGVNPRWKCLWRPYPRATAGLITSLDASCDGALDY
                     RASTPTPGTIEVWSPGVADRQPTVAGEGISDIDIAAVAGGFVVSATATGDYHVVLSPS
                     "
     gene            complement(1826..>2173)
                     /locus_tag="PKY13_16760"
     CDS             complement(1826..>2173)
                     /locus_tag="PKY13_16760"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HOT81646.1"
                     /translation="KGLTTDDLEINRTDKSIVITVPEPKLSEPNIDESKSKVITQDRG
                     LMTRLGELFDDAPLKGRDELDDVAVEELVKAADESELRSTAEENTRDFLTALANRLGY
                     EDVEVRFRPAAAP"
BASE COUNT          329 a          804 c          709 g          331 t
ORIGIN      
        1 tccacggcac cgcgatcggc gtcagcatcg tcgagccggg gttcatccgc gacgccggga
       61 tgttcgccga gtcgggcatc gcccttccca agggtgtccg caccaagtcg cccgccgacg
      121 tcgcccgcgg cgtcatcggc gccatcgagg gcgacaaggg cgaggtcttc gtcgcgccgc
      181 tcgagctccg attgtcggca accctggcga ccgtggcacc ggcgctcagc tcccggatcc
      241 aacgagccat cgataccgcc ggcatgaagg gaacgaagtg atgcaccgga tgaaccgctc
      301 ggttctcgcc acgctcctcg tcggagcact cgtcgtcggc ggctgcagct cgaccgacga
      361 cgccgcggac gacggcccca cctcgacccg cgccgagtcc acgaccacca gcgtgccggc
      421 gccaccggag ttgccggccc tccgcgccat ccgcggtgac caaccacgca tcgtcgacga
      481 ccaggaacgg caggtcatcc tgcgcggcgc caacctgaac tcgctcggcg actaccacca
      541 gggtgacccc gacgcggttc ccgtcgtgcc cgtgaccgac gccgactggg cggagatggc
      601 ggcgcacggg ttcaacgtcg tccgcctgct ggtcagttgg tcgaagctcg aaccggaacg
      661 cggcaccttc gacgaggagt acctcgccac cgtcgccgac gcggtcgacg ccgccgcggc
      721 gcacggcatc tacagcgtcg tcgacatgca ccaggacgcc tggggcaagt acatcgcctc
      781 acccgccggc gagacctgcc ccgacggcgg cgacccggcc atcggttggg acggcgctcc
      841 cgagtgggcc accctcaccg acggcgcctc gacctgcacg ttcggctcgc gggagagctc
      901 ccgtgccgtc atcaacgcgt gggactcgtt ctacgccaac cgcgacggca tcatggacga
      961 gctggtgcgg acgtgggcgt tcgtcgtcgg tggcctcggc gaccgtcctt cgatcgccgg
     1021 gtacgacctc ctgaacgaac ccaacgtcgg gagcggcgag accccggcga ccgaccagct
     1081 cggccgcttc tacgaccagg cgatcaccgc gatccgagcg gccgaggaac cgctcggtgt
     1141 ggatcggatc gtgttcttcg agttcaccgt tgccggccaa cccgttccga acgacttcac
     1201 cgccgacgac aacatcgtgt tcgctcccca ccactacggg gagtcgatcg gagccatccc
     1261 gatcgagggc ctgttcgact atctcgccag cctcgccgcc ggctaccgca ccgcgctgtg
     1321 ggtgggcgag tacggcttct tcgaagacac cgacgcggcg ggcgcgaagc tcgaacggta
     1381 cgccgccaag gaggacgcgt tggtgaccgc cggcgacgcc tggtggcagt ggcgccaggc
     1441 gtgcggcgac ccccactcgg tgggccggcc cggcgggacc tccgacccgg tgcagatcca
     1501 cctccagacc aaccgctgtc cgggcgacgt caacggcggc gtgaacccgc gttggaagtg
     1561 tctgtggcgt ccgtacccac gggccacggc cggcctcatc acgtcgctcg acgcttcctg
     1621 cgacggcgcc ctcgactacc gggcatcgac gccgacaccc ggcacgatcg aggtctggtc
     1681 acccggtgtc gccgaccggc agcccaccgt cgccggtgag ggaatcagcg acatcgacat
     1741 cgctgcagtc gccggcgggt tcgtcgtcag cgccaccgcc accggcgact accacgtggt
     1801 gctgtcgccg agctagcgct tcgactcagg gcgccgctgc cggacggaac cgcacctcga
     1861 cgtcctcgta tcccaatcgg ttggcgaggg ccgtgaggaa gtcgcgggtg ttctcctcgg
     1921 ccgtgctccg cagttcactc tcgtccgccg ccttcacgag ctcctccacg gcgacgtcgt
     1981 cgagttcgtc gcggcccttg agcggcgcgt cgtcgaacag ctcgccgagg cgcgtcatca
     2041 ggccgcggtc ctgggtgatc accttcgact tcgactcgtc gatgttcggc tcggagagct
     2101 tgggctccgg cacggtgatc acgatcgact tgtccgtgcg gttgatctcg aggtcgtcgg
     2161 tggtcaagcc ctt
//
LOCUS       DAOSOA010000427         2164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_50789_length_2164_cov_7.083452, whole genome shotgun sequence.
ACCESSION   DAOSOA010000427 DAOSOA010000000
VERSION     DAOSOA010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50789_length_2164_cov_7.083452"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKY13_16765"
     CDS             complement(<1..295)
                     /locus_tag="PKY13_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81647.1"
                     /translation="MASTLKRLLIGRPLATDEADHQRISKTIGLAVFSSDAISSTAYA
                     TGEVMLVLVGAAGLYKQAGLGVEDTPALLPAIAILVVVLLAIVANSYRQTIHAY"
     gene            468..>2164
                     /locus_tag="PKY13_16770"
     CDS             468..>2164
                     /locus_tag="PKY13_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81648.1"
                     /translation="MRTRTSLRRLQLIAVAATTVAIATACPAEPTGPFVPPVPSIAMN
                     PQLSGGYFSMPWPNDIRRSDSGMTDWTSLPGINTDIFTEPLPPIPLLPEIVAKGQTTV
                     NQFGRQTAVFFQANVELAAASLPAPDQTTSSGASVLLMDLATGELAPTVVVNQERADR
                     FRPAHLLTVLPYPGHPLRSDARYAALVFDDVTSTSGDALTPSATLAQLDQPYDPSMPM
                     TAAQHANLAGQYTDVKSAIANHTTHQLSDLVAFTVYRTQKTEREWDAIVAAMADVPTP
                     TVNVTSTGACTPSSRDAGASMSIVKATISLPIWRNGSFPYLFEGGKVVVQPNGKAQQF
                     GNRTAPVELMVPCSTMPAAGWPIVTHMDGTGGDEQIGTDIPIARRNGVLYGKISPLYG
                     DGVGDAGAILSPLGFSSPRAQAEVLFYNLLNPDSIRSNPLQQAAEQLMFTRALESFSV
                     PGTPFGQPVDVHGDASKVMITGHSQGAQTLPMIAAADPSIDGVISSSGSGGQYHTLAH
                     SPRRLNTLSLVTNYADRLDELNPLIQVVQTVFEASDGANFANDTNFLNYTNYDDTCSV
                     "
BASE COUNT          367 a          784 c          669 g          344 t
ORIGIN      
        1 gataggcgtg gatcgtctgc cggtagctgt tggcgacgat cgccaacagc accaccacga
       61 ggatcgcgat cgcgggcagc agcgccgggg tgtcctccac gccgaggccc gcctgcttgt
      121 agaggcccgc ggcgccgacc agcacgagca tgacctcgcc cgtcgcgtac gccgtcgaac
      181 tgatggcgtc cgacgagaac accgccagcc cgatcgtctt actgatccgc tgatgatccg
      241 cctcgtcggt ggccagaggc cggccgatca gcaggcgttt gagagttgag gccacggtgg
      301 gagcgtacgt cgcgtgttat ggaggcgtta aatttcgcga gcggactgct tgcctcaggc
      361 tacgccccgt tgctcgatca ggccatggaa acgttgatga tccgggtcgg gagtagtaga
      421 accgaacctc aggccgaaca ggttcgttcg gcatgggggg attcccgatg agaacacgca
      481 cgtccttgcg gcgcctgcag ctgattgcgg tcgctgcgac caccgtggcg attgccacgg
      541 cgtgcccggc cgagccgacg gggcccttcg tcccgccggt gcccagcatc gccatgaacc
      601 cgcagctgtc gggtggctac ttctcgatgc cgtggcccaa cgacatccgt cgatccgatt
      661 cggggatgac cgactggacg tcgctgcccg gcatcaacac cgacatcttc accgagccgc
      721 tcccgccgat tccgctcctg cccgagatcg tcgccaaggg tcagacgacg gtcaaccagt
      781 tcggacgcca gaccgcagtg ttcttccagg ccaacgtcga gctggccgcc gccagtctcc
      841 cggcgccgga tcagaccacg tcatcgggcg cgtcggtgct gctcatggat ctggcgacgg
      901 gggagctcgc tcccaccgtc gtcgtcaacc aggagcgcgc cgaccggttc cggcccgccc
      961 acctcctgac cgtcctgccc taccccggcc atccgctgcg gtccgacgca cggtacgccg
     1021 cgctcgtgtt cgacgacgtg acgtcgacga gcggcgacgc gctcacgccg tcggccacgc
     1081 tggcgcaact cgatcagccc tacgacccgt ccatgccgat gacggcggcg cagcacgcca
     1141 acctggccgg gcagtacacc gacgtgaagt cggcgatcgc gaaccacacg acccaccagt
     1201 tgtcggatct cgtcgccttc accgtgtacc gcacccagaa gaccgagcgg gagtgggacg
     1261 ccatcgtggc ggcgatggcc gacgtgccga cgcccacggt caacgtcacg tcgacgggcg
     1321 cgtgcacacc gtcgagtcgc gacgccggtg cctcgatgtc gatcgtcaag gcgaccatca
     1381 gcctcccgat ctggcgcaac ggttcgttcc cgtacctgtt cgagggcggc aaggtcgtcg
     1441 tgcagcccaa cggcaaggcg cagcagttcg gcaaccgcac cgcgccggtc gagctgatgg
     1501 tcccctgctc cacgatgccg gccgccggtt ggccgatcgt gacccacatg gacggcaccg
     1561 gcggcgacga gcagatcgga accgacattc ccatcgcccg ccgcaacggt gtgctctacg
     1621 gcaagatctc gccgctctac ggcgacgggg tcggtgacgc cggggcaatc ctgtcccccc
     1681 tcgggttctc gtcgccccgg gcgcaggccg aggtgctctt ctacaacctg ctcaaccccg
     1741 actcgatccg gtccaacccg ctccagcagg ccgccgagca gctgatgttc acgagagccc
     1801 tcgagtcgtt ctccgtcccg ggtacgccgt tcggtcagcc ggtcgacgtg cacggtgacg
     1861 cttcgaaggt gatgatcacc ggtcacagcc agggcgcgca gacccttccg atgatcgcgg
     1921 cggccgaccc gtcgatcgac ggagtgatct cgtcgtcggg ctccggcggg caataccaca
     1981 ccctggcgca ctcaccccgc cggctgaaca ccctcagcct cgtgaccaac tacgccgacc
     2041 gcctcgatga actcaacccg ctcatccagg tcgtgcaaac agtgttcgag gcgtccgacg
     2101 gcgccaactt cgccaacgac accaacttcc tcaactacac gaactacgac gacacctgtt
     2161 cggt
//
LOCUS       DAOSOA010000428         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_50926_length_2160_cov_6.503563, whole genome shotgun sequence.
ACCESSION   DAOSOA010000428 DAOSOA010000000
VERSION     DAOSOA010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50926_length_2160_cov_6.503563"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKY13_16775"
     CDS             complement(<1..530)
                     /locus_tag="PKY13_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOT81649.1"
                     /translation="MKIAAIQHDIVWKDAAATHDHVDPLIELAARSGASLIVLSEMFS
                     TGFSTVAADIAQAEDGPTTEFVRQRAASHGVWITGSIAVVDGATGEARNRFTAAGPDG
                     QLVTYDKRHPFTYAGEHLEFVPGDDLVSFEVEGLRVTPFVCYDLRFADDFWARGPDTD
                     LFLVVANWPAARRRHWS"
     gene            complement(625..1116)
                     /locus_tag="PKY13_16780"
     CDS             complement(625..1116)
                     /locus_tag="PKY13_16780"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOT81650.1"
                     /translation="MSTSRLFSFPDPVNEVSARLVAAGVVVMAAIILFAGQTWLVAVL
                     AYGFVARVLTGPTLSPLGRVVTDVITPRLPFAARPTAGPPKRFAQGIGATLSTAAVVA
                     HFGFGSATVANVLVAMILVAASLEAFVGFCLGCTIFRGLMRLGVIPETVCEACNNLQL
                     RHA"
     gene            complement(1185..1508)
                     /locus_tag="PKY13_16785"
     CDS             complement(1185..1508)
                     /locus_tag="PKY13_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81651.1"
                     /translation="MTFLKTTCDQCGEIELPVDRVVLRICENEDSGVCVIRCPSCGSR
                     FVKEANDAMIVMLLAVGIEVSMWTAAVTGPVDDGLPAITHDDLVTFRRHLGEDSELLR
                     HLEAI"
     gene            1710..>2160
                     /locus_tag="PKY13_16790"
     CDS             1710..>2160
                     /locus_tag="PKY13_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT81652.1"
                     /translation="MTQAALTSDLEFAVDVVRQAGEFTMTHFRAAELEIIRKADGSPV
                     TLADQGAESLMRRLIGARFPDDAILGEEEGEKAGTSGRRWVIDPIDGTEAFTHGVALY
                     SNLLYLEDQHGPAVGVINVPAIGEMVWAGRGLGCFLNDIPCRVSSKAD"
BASE COUNT          404 a          725 c          716 g          315 t
ORIGIN      
        1 gaccagtgcc gacgtcgggc cgccggccag ttggcgacga ccaggaacag gtcggtgtcg
       61 ggtccacggg cccagaagtc gtcggcgaag cggaggtcgt aacagacgaa gggggtgacc
      121 cgaagaccct ccacctcgaa ggagacgagg tcgtctccgg gtacgaactc gaggtgctcg
      181 cctgcgtagg tgaagggatg ccgcttgtcg taggtcacga gctgtccgtc gggaccggca
      241 gccgtgaacc gattgcgggc ctcaccggtc gcgccatcga cgacggcgat cgaaccggtg
      301 atccatacac cgtgcgacgc cgctcgctgc cgaacgaact cggtcgtcgg gccgtcctcg
      361 gcctgggcga tgtcggcggc gaccgtcgag aacccggtcg agaacatctc cgacaggacg
      421 atcaacgatg ccccggaccg ggctgccagt tcgatgagcg gatcgacgtg atcgtgggtc
      481 gcagctgcgt ccttccacac gatgtcgtgc tggatggccg cgatcttcat ggcgcgcccc
      541 cgccgctcga caacaacaac atcacgatga ggctagcggg gaggcggcgc gaaacccgcc
      601 cggacgacgt tgcgtcggaa attctcaggc gtgtcggagc tgcaagttgt tgcaggcttc
      661 gcagacggtc tcggggatga ctccgagacg catcaggcca cggaagatgg tgcacccgag
      721 gcagaatccg acgaacgctt ccagactggc cgccaccagg atcatggcga ccagcacgtt
      781 ggcgaccgtc gccgaaccga acccgaaatg cgcaacgacg gccgctgtcg agagggtggc
      841 gccgatgccc tgcgcgaacc gcttgggcgg cccggcagtg ggccgagcgg cgaacggcag
      901 gcgcggcgtg atcacatcgg tcacgacacg cccgagcggg ctgagcgtgg gaccggtgag
      961 cacccgggcc acgaacccgt aggcgaggac cgcgacgagc caggtctggc cggcgaacag
     1021 gatgatcgcc gccatgacga ccactccggc cgccaccaag cgtgcggaga cctcgttgac
     1081 cggatcggga aaggagaaca gtcgtgacgt actcacagtt ccaggatacg ccccgatacc
     1141 cgaccaaaca agtcaagatt acggaatcca tcaggattcg acgatcagat tgcctcgagg
     1201 tgacgcagca gttcggaatc ctcgccgagg tggcggcgga aggtcacgag gtcgtcgtgg
     1261 gtgatggccg gtaggccgtc gtcgaccggt ccggtcacgg cggccgtcca catcgacacc
     1321 tcgataccga ccgccagcag catcacgatc atggcgtcgt tcgcttcttt cacgaagcgg
     1381 cttccgcaac tggggcaccg gatcacgcag acaccgctgt cctcgttctc gcagatccgc
     1441 aggacgaccc ggtcgaccgg cagttcgatc tcgccgcact ggtcgcacgt cgtcttcagg
     1501 aaggtcacct caccacccca catctgccca ttggtcgccg tcgatctctc cgggatcgct
     1561 caccgagaac acctatcgac cgtcgcgaac cggagatgag gcgcgcggac acgattgggt
     1621 cggagcgccc atcgacgcgc caccccgagc agcgatgcac cggggaccga ggtcgagaac
     1681 ccgtcgtcgg gtgagcgaca atggacccca tgacccaggc cgctctcacc tccgatctcg
     1741 agttcgccgt cgacgtggtt cgccaggccg gcgagttcac gatgacccac ttccgcgccg
     1801 ccgagctcga gatcatccgc aaggccgatg gctcgccggt gacgctcgcc gatcagggag
     1861 ccgagtcgct gatgcggcgc ctcatcggtg cccgcttccc cgacgacgcg atcctcggcg
     1921 aggaggaggg cgagaaggcc ggcacgagcg gccgccgctg ggtcatcgac ccgatcgacg
     1981 gcaccgaggc cttcacccac ggcgtcgccc tctactccaa cctgctgtac ctcgaggacc
     2041 aacacggtcc ggccgtcggc gtgatcaacg tgccggccat cggtgagatg gtgtgggccg
     2101 ggcgtggcct cggctgcttc ctcaacgaca tcccgtgccg cgtgtcgagc aaggccgacc
//
LOCUS       DAOSOA010000429         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_52031_length_2126_cov_7.073877, whole genome shotgun sequence.
ACCESSION   DAOSOA010000429 DAOSOA010000000
VERSION     DAOSOA010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52031_length_2126_cov_7.073877"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKY13_16795"
     CDS             complement(<1..623)
                     /locus_tag="PKY13_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT81653.1"
                     /translation="MSFISVEHHDRVAVVTLDDPKRRNALNLELCAELVEAVEQLEAD
                     DGTGAIVVTGNPPAFCAGADLSQLGASQREGLKQIYAGFLRVASSSLPTIAAVNGAAV
                     GAGMNLALACDVRLAGRSARFDTRFVDLGLHPGGGHTWMMRRIVGPQATAAMVLFGQI
                     LDGAEAERVGLVWKCVDDDTLLAESIAMAARAASGPPELVPVIKQTIA"
     gene            complement(663..1316)
                     /locus_tag="PKY13_16800"
     CDS             complement(663..1316)
                     /locus_tag="PKY13_16800"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT81654.1"
                     /translation="MWSRRSQVKAEVPGVAPANVAGATLIGRRLVLRPLVAADFDQWR
                     EVRLRCEDWLTKWEPARIEGAPDTVGDRQAFAARCGARTREIQMGSGYGFGMFVDGFF
                     AGEININSVHRGPFQSAYVGYWIDERHAGHNYTPEAVALVLRFAFEQMMLHRIQISII
                     PRNQASRRVAEKLELRNEGVAERYLEINGVWEDHVRYAITSEEWAVRRDEILAGWAY"
     gene            complement(1323..>2126)
                     /locus_tag="PKY13_16805"
     CDS             complement(1323..>2126)
                     /locus_tag="PKY13_16805"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81655.1"
                     /translation="EYREVMDRYELAGVRATATSAARDASNRADFFGPATEVLGVEPE
                     LLSGEDEARLSFLGATADLDPLDGPFLVVDIGGGSTEFAYGRANADRVIEFQAGTSID
                     VGCVRMTERYLHSDPPGADELSSCLSVLQLHWDDVRVDVPQAFEAARLVGLAGTVVNV
                     AAVELGLAAYDRNATHHFVLTKEAVEDVFRTLATESLEDRKHNPGLEEARADVIVGGL
                     CILVSIMRTFGFAECLVSESDILDGLIASQASPPGGGLPRVTGPETPVG"
BASE COUNT          348 a          738 c          725 g          315 t
ORIGIN      
        1 gcgatcgtct gcttgatgac cggcaccagc tcgggcggcc ccgaagcagc gcgcgccgcc
       61 atcgcgatcg actcggcgag cagggtgtcg tcgtcgacgc acttccacac cagcccgacg
      121 cgctccgcct ccgctccgtc gaggatctgc ccgaacagca ccatggccgc cgtggcctgc
      181 gggccgacga tgcgccgcat catccaggtg tggccgccgc cggggtgcag gccgagatcc
      241 acgaaacggg tgtcgaaccg ggcggagcga ccagcgagcc gcacgtcgca ggcgagcgca
      301 aggttcatac ccgctccgac ggcggcgccg ttgaccgcgg cgatggtcgg gagcgacgat
      361 gacgctacgc ggaggaaccc ggcgtagatc tgcttgagac cctcgcgctg tgacgctccc
      421 agttggctca ggtcggcgcc ggcgcagaac gctggcggat tgccggtgac gacgatcgct
      481 ccggtgccgt cgtcggcctc gagctgctcg acggcctcga cgagttcagc gcagagctcg
      541 aggttgaggg cgttgcggcg cttggggtcg tcgagggtga cgacggcgac acgatcgtgg
      601 tgttcgacgg agatgaacga catgggagtc agtctcccag gactcgctgt gtccgggaca
      661 gctcagtagg cccagccggc caggatctcg tcgcggcgaa ccgcccactc ctcggaggtg
      721 atcgcgtaac ggacgtggtc ttcccacacc ccgttgatct cgaggtaccg ctcggccaca
      781 ccctcgttgc ggagctccag cttctcggcg acgcggcgac tggcctggtt gcgagggatg
      841 atcgagatct ggatgcggtg caacatcatc tgctcgaacg cgaaccggag cacgagcgcc
      901 acggcctcgg gcgtgtagtt gtgtccggcg tgccgctcgt cgatccagta cccgacgtac
      961 gcgctctgga acgggccccg gtgcaccgag ttgatgttga tctcgccggc gaagaacccg
     1021 tcgacgaaca tgccgaaccc gtaccccgaa cccatctgga tctcgcgggt ccgagcaccg
     1081 caccgggcgg cgaaggcctg ccggtcaccc accgtgtcgg gcgccccctc gatgcgggcc
     1141 ggctcccact tggtgagcca gtcctcacag cggaggcgga cctcacgcca ctggtcgaag
     1201 tcggcggcga ccaacggccg gagcaccagc cgacggccga tgagcgtcgc tccggccacg
     1261 ttcgccggtg caaccccggg gacctccgcc ttcacctgtg agcgccggga ccacatatgt
     1321 gctcagccta ccggggtctc cggcccggtg acccggggaa ggccaccacc tgggggactt
     1381 gcctggctgg cgatcagacc gtcgaggatg tcgctctcgg acacgaggca ctcggcgaag
     1441 ccgaacgtgc gcatgatcga cacgaggatg cagagaccac ccacgatcac atcggcgcga
     1501 gcctcctcga ggccggggtt gtgcttacgg tcttcgagcg actcggtggc caaggtgcgg
     1561 aagacgtcct cgaccgcctc cttggtcagc acgaagtggt gcgtggcgtt gcggtcgtag
     1621 gcagcgagcc ccaattcgac cgcagcgacg ttgacgaccg tgcccgcgag accgaccagc
     1681 cgagctgctt cgaacgcctg cggcacgtcg acgcgaacgt cgtcccagtg caactgcagc
     1741 accgagaggc acgacgagag ttcgtcggcg cccggcgggt cggagtggag gtagcgctcc
     1801 gtcatccgga cgcacccgac gtcgatggac gttcccgcct ggaactcgat gacgcgatcg
     1861 gcgttggcgc gtccgtaggc gaactcggtg gagccaccgc cgatgtcgac cacgaggaac
     1921 ggaccgtcga gcggatcgag atcggccgtc gccccgagga agctcagccg agcctcgtcc
     1981 tcgccactca ggagttccgg ctccaccccg agcacctcgg tcgccggacc gaagaagtcg
     2041 gctcggttcg acgcgtcgcg cgccgccgac gtcgcagtgg cccgcacgcc ggcgagctcg
     2101 taccggtcca tcacctcccg gtactc
//
LOCUS       DAOSOA010000430         2044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_54881_length_2044_cov_3.125189, whole genome shotgun sequence.
ACCESSION   DAOSOA010000430 DAOSOA010000000
VERSION     DAOSOA010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54881_length_2044_cov_3.125189"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2044
                     /locus_tag="PKY13_16810"
     CDS             <1..>2044
                     /locus_tag="PKY13_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81656.1"
                     /translation="VNDGPGPAYYIEFDDRPATSSYYGYQVTWDVRSNTTPAPATVDS
                     APTNGSKIAWTLPELAEGASYTASFEARTSLPDYASNVQVSTYEYLGSRFSNAAKITS
                     YDPDDFGTVHIADNANWWSTWNIQKKISTTWMELTTPRISLAKQVNVQGGVDPATRFD
                     SPTCASWQRLTVSGHELCYNSNSGEPFDGQQPLEYHLNVMLTHPELLRTVDITDRLPL
                     GWSYVPGTARLEQAGWGADGELVTTTTPLFDPTTTPATETTCQWYNINGNGDRLQWVF
                     SRAATDQSNALWDTAMRWTGPSDNPNARFDATYRITFDAAPNAMWTGCKPQSTPGWDP
                     IGPSIENQATMAVTTTADTSGSAYATTVVRALDPPRFVKSPKAGYVSANGDAVFTLDT
                     ENVQKFGIDGVTVTDTINDWSVAAGAPLYQPGSATITDEFGDPVAATETIVHRSTGPG
                     DPSIISWTYDQAPAYNAGSEPRITNWSCPVSATPARPYWAECASRVRVTLSLRVPDEE
                     ADGYRYTNEATAVFPERLYDPTASNYDRFGSFTYGATLTDTGAYTVVNPSPPPAPSKW
                     GTSWATPNDVVNYYVEATVPAGTTYHDLAYTDVLPDGLTDIEIAAVEVFDQATGSPAP
                     FPTGTHLGPVVDPATHTTTVGVFFGEVPGVDLTRTIRIRIRARVAERHPALWTKTQ"
BASE COUNT          363 a          784 c          602 g          295 t
ORIGIN      
        1 tcgtcaacga cggccccgga ccggcgtact acatcgagtt cgacgaccgt cccgcgacgt
       61 cgtcctacta cggctaccag gtgacctggg acgtgcggtc gaacaccacc ccggcgccgg
      121 ccaccgtcga ctcggcgccc accaacggct cgaagatcgc ctggacactg cccgaactcg
      181 ccgagggcgc ctcgtacacg gcatcgttcg aggcgaggac gtcgctgccc gactacgcgt
      241 ccaatgtgca ggtcagcacc tacgagtact tgggctcccg gttctcgaac gccgccaaga
      301 tcacctccta cgatcccgac gacttcggta ccgtccatat cgccgacaac gcgaactggt
      361 ggtcgacctg gaacatccag aagaagatct ccaccacctg gatggagctc acgaccccga
      421 ggatctccct cgccaagcag gtgaacgtgc aaggtggcgt cgaccctgca acgcgcttcg
      481 attccccgac ctgcgcctcg tggcagcgcc tcacggtcag cggccacgag ctctgctaca
      541 actccaactc cggcgagccg ttcgacgggc agcaacccct cgagtaccac ctcaacgtga
      601 tgctgacgca tccggaactc ctccggacgg tcgacatcac cgaccggctc ccgctgggtt
      661 ggagctacgt cccgggaacg gcccgattgg agcaggccgg gtggggcgcc gacggtgaac
      721 tcgtcaccac gacgacgccg ctcttcgacc ccacgaccac gccagccacc gagaccacct
      781 gtcagtggta caacatcaac ggcaacggcg accgcctcca gtgggttttc tcccgtgccg
      841 cgaccgacca gtccaacgcc ctgtgggaca ccgccatgcg ctggaccggt ccgtccgaca
      901 accccaatgc ccgattcgat gcgacgtatc ggatcacgtt cgacgccgcc ccgaacgcga
      961 tgtggaccgg ctgcaagccg cagtcgacac cggggtggga ccccatcgga ccttccatcg
     1021 agaaccaggc cacgatggcg gtcaccacga ccgccgacac gtcggggtcg gcgtatgcga
     1081 ccaccgtcgt ccgcgccctc gacccgccac gcttcgtcaa gtccccgaag gcgggctacg
     1141 tgagcgccaa cggcgacgcc gtgttcacgc tcgacaccga gaacgtgcag aagttcggca
     1201 tcgacggagt caccgtgacc gacacgatca acgactggtc ggtcgcggcg ggcgctccgc
     1261 tgtaccagcc gggctcggcg acgatcaccg acgagttcgg ggatccggtc gccgccaccg
     1321 agaccatcgt gcaccgctcg accggtccgg gcgacccgtc gatcatcagc tggacctacg
     1381 accaggctcc ggcgtacaac gccggctccg agccccggat cacgaactgg tcctgcccgg
     1441 tgtccgccac gccggcccgc ccctactggg ccgagtgcgc ctcccgcgtc cgcgtcacgc
     1501 tcagcctgcg ggttcccgac gaggaggcgg acggctaccg ctacaccaac gaggccaccg
     1561 cggtcttccc cgagcggctc tacgacccga cggcgtcgaa ctacgacagg ttcggttcct
     1621 tcacgtacgg cgccacgctc accgacaccg gtgcctacac cgtggtcaac ccgtcgccgc
     1681 cgcctgcccc ctccaagtgg ggcacgagct gggcgacgcc gaacgatgtg gtcaactact
     1741 acgtcgaggc aaccgtcccg gccggcacga cctaccacga cctggcgtac accgacgtcc
     1801 tgccggatgg tctgaccgac atcgagatcg ccgctgtcga ggtgttcgac caggccaccg
     1861 gttctcccgc gccgttcccg accgggaccc acctcggccc cgtggtcgac cctgccaccc
     1921 acacgacgac cgtcggcgtg ttcttcggtg aggttccggg tgtcgatctc acccggacga
     1981 ttcgcatccg catccgggcc cgcgtggccg agcggcaccc cgcgctgtgg acgaagaccc
     2041 agca
//
LOCUS       DAOSOA010000431         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_55768_length_2021_cov_5.336724, whole genome shotgun sequence.
ACCESSION   DAOSOA010000431 DAOSOA010000000
VERSION     DAOSOA010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55768_length_2021_cov_5.336724"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKY13_16815"
     CDS             complement(<1..697)
                     /locus_tag="PKY13_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOT81657.1"
                     /translation="MRGIISAAGYLPYWRLERSAIGAFHGGRGSGTRTVASYDEDTTT
                     LAVEAGRLALKGTTAEPETLWFATSTPTYAEKTNATVIHAALRLPAATAAFDSGSAVR
                     SGVGALRAALRSSESAVLVTAADIRTGPANSPDESAGGDAGAAILVGDESAGPVIAEY
                     LGGASITREFLDRWRTPGDARTKGWEERFGETQYLELGMTAWIDALSATGVGAASGLD
                     GTESQEAVDHVLIA"
     gene            782..>2021
                     /locus_tag="PKY13_16820"
     CDS             782..>2021
                     /locus_tag="PKY13_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOT81658.1"
                     /translation="MRLGDVANGDAQQYGKPLEGVRILAAEQMQALPYATQLLARLGA
                     EVVKVEHPTAGESGRGAQPAMTDPEGRPVGATFLRNNLNKRSVGIDLKHPRGRELFIE
                     LAAKFDIVGENFKAGTMDRLGLGYDALSAIYPGLIFVSVSGFGNLGESPYAAWPAYAM
                     VPEAMSGIYDYARRGDEPPRVVPVGALGDISTGLFAVIGILAALRHRERTGLGQYVDI
                     AMLDAMVSMTDVVTNLWSLGERPQEGPNGIMNSFRASDGYFAMQVVREKQFETLAELI
                     GAPEWITDERFSTRVGWGRHTDSVIRPRLEAWAAQYSRLEVCDLLGRANIPSGPVHTA
                     SEVIADPHVAARHMLVEMERTDDGPDPVLIPGNPVKLSNVAEGPETRVPWVGEHTDDV
                     LRAELGLDEDALADLRSDGVI"
BASE COUNT          305 a          731 c          697 g          288 t
ORIGIN      
        1 cggcgatcag gacgtggtcg acggcctcct gcgactcggt gccgtcgaga cccgaggccg
       61 cgcccacacc ggtggccgag agcgcgtcga tccaggccgt catgccgagt tcgaggtact
      121 gggtctcgcc gaagcgctct tcccagccct tggtgcgagc atcgccgggc gtgcgccagc
      181 ggtcgaggaa ctcgcgggtg atcgaggcgc caccgaggta ctcggcgatg accggtccgg
      241 ccgactcgtc gcccacgagg atcgcggcgc cggcgtcgcc gcccgccgac tcgtcgggcg
      301 agttcgccgg gcccgtgcgg atgtcggcgg cggtcacgag gacggcagac tccgacgacc
      361 ggagggcggc gcgcagcgca ccgaccccgg accggaccgc tgagccgctg tcgaaggccg
      421 ccgtcgccgc cgggagtcgc agcgccgcgt ggatcacggt ggcgttcgtc ttctcggcgt
      481 aggtcggagt ggacgtcgcg aaccacagtg tctcgggctc agccgtcgtg cccttcaggg
      541 cgaggcggcc cgcctcgacc gccagggtcg tcgtgtcctc gtcgtaggag gcgacggtgc
      601 gggtgccgct gccccgacct ccgtggaacg ccccgatcgc agaacgttcg agccgccaat
      661 agggcaggta ccccgccgcg ctgatgattc ctcgcatccg gccagagtac ctgtttctga
      721 cactccgtca gaaacgcggc ggtcgagcgg caccggccgc accccgtacg atcggtcgcc
      781 tatgcgactc ggcgacgtgg ccaacggtga tgcccagcag tacggcaagc ccctcgaagg
      841 tgtccggatc ctggcggcgg agcagatgca ggccctcccc tacgcgacac agctgctggc
      901 gcgcctgggc gccgaggtgg tcaaggtcga gcacccgacc gccggtgagt ccggccgcgg
      961 cgcgcaaccg gcgatgaccg atcccgaagg tcgcccagtc ggggcgacct tcctgcgcaa
     1021 caacctcaac aagcggtcgg tcggcatcga cctcaagcat ccccgcggac gcgagctctt
     1081 catcgaactg gctgcgaagt tcgacatcgt cggcgagaac ttcaaggccg gcaccatgga
     1141 ccgcctcggc ctcggctacg acgcgctgtc ggcgatctac cccggtctca tcttcgtgtc
     1201 ggtgtccgga ttcgggaacc tcggcgagtc gccctacgcc gcctggcccg cctacgcgat
     1261 ggtgcccgag gccatgtcgg gcatctacga ctacgcccgc cgcggcgacg aaccaccgcg
     1321 ggtcgtgccc gttggagcgc tcggcgacat cagcaccggg ctcttcgccg tgatcggcat
     1381 cctcgcagcg cttcgccacc gcgagcgcac cggcctcggg cagtacgtcg acatcgcgat
     1441 gctcgacgcc atggtctcga tgaccgacgt cgtgaccaac ctgtggtcgc tcggcgagcg
     1501 cccccaggag ggaccgaacg gcatcatgaa cagcttccgg gcgtccgacg gctacttcgc
     1561 catgcaggtg gtgcgcgaga agcagttcga gacgctggcc gagctgatcg gcgcaccgga
     1621 atggatcacc gacgagcgct tctcgacccg cgtgggttgg ggccgccaca ccgattcggt
     1681 gatccgcccg cggctcgagg cgtgggcggc tcagtacagc cggctcgagg tgtgcgacct
     1741 cctcggccgg gcgaacatcc cgtcgggccc ggtgcacacc gcgtccgagg tgatcgccga
     1801 cccccacgtc gcggcccggc acatgctcgt cgagatggag cgcaccgacg acggccccga
     1861 cccggtactg atacccggca acccggtgaa gctctcgaac gtcgccgagg gccccgagac
     1921 ccgcgtgccg tgggtcggcg agcacaccga cgacgtgctg cgcgctgagc tcggcctcga
     1981 cgaggacgcg ctcgccgacc tgcgatccga cggcgtgatc a
//
LOCUS       DAOSOA010000432         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_58827_length_1944_cov_5.994706, whole genome shotgun sequence.
ACCESSION   DAOSOA010000432 DAOSOA010000000
VERSION     DAOSOA010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58827_length_1944_cov_5.994706"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKY13_16825"
     CDS             complement(<1..190)
                     /locus_tag="PKY13_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HOT81659.1"
                     /translation="MSDELQGKRVAFIVANEGVEEVELTDPWRAVERAGGTAVLLAPE
                     AGKVQAYHHLDRAESFEVD"
     gene            341..1549
                     /locus_tag="PKY13_16830"
     CDS             341..1549
                     /locus_tag="PKY13_16830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT81660.1"
                     /translation="MSLSDVSRDDSVRPVVLSDAPTWCDSEVARRAWQIGVEFAAPHA
                     DDVDRSARFPKEAVAEIRASGLLAAMIPTELGGGGASLAEVATAVRAFAAHCSATALV
                     FAMHQIEIWYLVECGHTPELQDLLRSVAEHGTLIANANAEVGLGGNIGQSVCAVERAD
                     GRFHLEKDALAISYGAHADAIMLTARRAPDAEPTDQVIVACRPGSFTLEQTSTWDTMG
                     LRGTCSCGFRLTVDEDEAMILPVSWAGVANKSLGASVILLNSAWLGIAESAAATAHRY
                     VRASARRTVGVTPATAPLLAEMAVTLTEARSVMANAVARQAAAHGTDAVDDATLILAL
                     RSLKLSMARLAIEVVAAASQICGLVGYKRDSPYSLDRHHRDITGAPLMANNLRFLGDN
                     AQLLLVMKQI"
     gene            complement(1533..>1944)
                     /locus_tag="PKY13_16835"
     CDS             complement(1533..>1944)
                     /locus_tag="PKY13_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81661.1"
                     /translation="GINAVYAERNGVSIRHLAASVALGLPIDPPPFVAERSDGPFTYQ
                     HFVQPPVSATGLVSIGPTAELIGRDGIGSVTVNRSPGDALDWRAGSQEYVLRVGGTAV
                     DRAALAAVPSTISGLIEITYASSPPGHDGVRSAS"
BASE COUNT          278 a          698 c          660 g          308 t
ORIGIN      
        1 cgtcgacctc gaacgactcg gcacggtcga ggtggtggta ggcctgcacc ttgcccgcct
       61 ccggcgccaa cagcacggcg gtgccgccgg cccgttcgac cgccctccac gggtcggtca
      121 gctcgacttc ctcgacccct tcgttggcga cgatgaacgc cacgcgcttg ccctgcaatt
      181 cgtcactcac ctgttcgttc cttccggctc gttggttggt ggcacctacc caactgcgct
      241 ggccgaccat gcccgagatc tcggaattcc cccgttcggg gtcgcctcgg cgggcggatc
      301 gaggttggcg cgatccccca aagcggtgca ccctgtcgaa atgagcttgt ccgacgtgag
      361 ccgtgacgat tccgttcgcc cggtggtgct ctcggacgca ccgacgtggt gtgacagtga
      421 ggtggcccga cgcgcctggc agatcggggt ggagttcgcc gccccccatg ccgacgacgt
      481 cgatcgttcc gcccggtttc cgaaggaagc cgttgccgag atccgggcat cgggcctgct
      541 cgcggcgatg atcccgaccg agctcggcgg gggtggggca tcgctcgccg aggtcgcgac
      601 ggcggtgcgc gcgttcgccg cgcactgctc ggcgacggcg ctggtgttcg ccatgcacca
      661 gatcgagatc tggtacctcg tcgagtgcgg ccacaccccc gagctccagg acctgctgcg
      721 cagcgtcgcc gagcacggca ccctcatcgc caacgcgaac gccgaggtcg gcctcggtgg
      781 caacatcggc cagagcgtgt gtgccgtgga gcgtgccgac ggccgcttcc acctcgagaa
      841 ggacgcgttg gccatctcct atggcgccca cgccgacgcc atcatgctca cggcgcgtcg
      901 cgcgcccgat gccgaaccga ccgaccaggt gatcgtcgcc tgccggcccg ggtcgttcac
      961 gctggagcag acgtcgacct gggacacgat gggactccgt ggcacgtgct cctgcggatt
     1021 ccgcctgacc gtcgacgagg acgaggcgat gatcctgccg gtgagctggg cgggcgtggc
     1081 aaacaagagc ctcggcgcct ccgtgatcct cctcaactcg gcatggctcg gcatcgccga
     1141 gtcggcagcc gccaccgccc accgctacgt gcgtgcgtcg gcccgtcgca ccgtcggcgt
     1201 cacgccggcg acggcccccc tcctcgccga gatggcggtc accctcaccg aggcccgcag
     1261 cgtcatggcc aatgccgtcg cccgtcaggc cgcggcgcac ggcaccgatg ccgtcgacga
     1321 cgcgacgctg atcctcgcgc tgcgcagcct caagctgtcg atggcccggc tggcgatcga
     1381 ggtcgtggcc gcggcgtcac agatctgtgg tctggtcggc tacaagcgcg actcgccgta
     1441 ttcgctcgac cggcaccacc gcgacatcac gggcgctccg ctcatggcga acaacctgcg
     1501 cttcctcggc gacaatgctc agctcctgtt ggtcatgaag cagatctgac gccgtcgtgg
     1561 cccgggggcg acgacgcgta cgtgatctcg atcagtccgg agatcgtcga cggcactgcg
     1621 gcgagtgccg cccggtcgac ggcggtccct ccgactcgca gcacgtactc ctggctgccg
     1681 gcgcgccagt cgagggcatc gccgggggag cggttcacgg tgaccgaacc gatgccgtcg
     1741 cggccgatca gctcggcggt cggtccgatc gacacgagac ccgtcgccga gaccggcggc
     1801 tgcacgaagt gctggtaggt gaacggcccg tcggaccgct cggcgacgaa cgggggcgga
     1861 tcgatcggca ggccgagcgc gaccgacgct gcgaggtggc ggatcgagac gccgttgcgt
     1921 tccgcgtaca cggcgttgat gcca
//
LOCUS       DAOSOA010000433         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_66410_length_1784_cov_3.155003, whole genome shotgun sequence.
ACCESSION   DAOSOA010000433 DAOSOA010000000
VERSION     DAOSOA010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66410_length_1784_cov_3.155003"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKY13_16840"
     CDS             <1..495
                     /locus_tag="PKY13_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81662.1"
                     /translation="TAEQVRAYVDSIHRRSDGSWTATPPPGFEPFPLARPADAETTRK
                     LIGRGTGINGAELNFSLLDSLERVTVDVHEPDHAVAANMIAMFMTMGPVERMTTRDVT
                     WYRSRSADGGQHRVAIAGTATRPTVIVASSALTDDQLFEIATSLRRASAAEVEAVFAP
                     LLDG"
     gene            568..>1784
                     /locus_tag="PKY13_16845"
     CDS             568..>1784
                     /locus_tag="PKY13_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81663.1"
                     /translation="MTNTSSPFTTHTATGRAVETDLRRALIALQVVMAELDPAAIPLP
                     DATAVFTLFADVRRLAGNGQTLMAGRVKEAREWARSGHATVEDWIANTTGSSVGAAKD
                     QVRTSERLDQLPDTTEQMRTGGVSPEQADIVADAAAVNPASEGDLLERARRDSNKALR
                     DEAARRKAQARGESANEKAQRHRAERSARFYNDRDGRRCLYAQGTAADLAALEAAIDR
                     AVDRRYRDTGSTGRREGRDRYAFDALTALADSGTGDPKRVQPKHLALIRVDLAALVRG
                     TVDEGEVCEIAGVGPISVDEARRLLGDSILHLVSENGVDVQNVTHLGRGPNAVQQVAL
                     LWRTRQCTVMGCNRTHTENDHRVDFADTRHTRVDEIDQMCDHHHDLKTYRDWVLVPGT
                     GRREMIGPHDPRHP"
BASE COUNT          303 a          691 c          559 g          231 t
ORIGIN      
        1 accgctgagc aggtgcgggc ctacgtcgac agcatccacc gccgatccga cggcagctgg
       61 acggcgacgc cgccgccggg cttcgagccc ttcccgctcg cccgacccgc cgatgccgag
      121 acgacccgaa agctcatcgg gcgcggaacc ggcatcaacg gggccgagct caacttctca
      181 cttctcgaca gcttggagcg ggtcaccgtc gacgtgcacg agcccgacca cgccgtcgcc
      241 gccaacatga tcgccatgtt catgaccatg ggccctgtgg agcgaatgac gacgagggac
      301 gtcacctggt accggtcacg atcggccgac ggcggccagc accgcgtcgc gatcgccggt
      361 acggcgacac ggccgacggt catcgtcgcg tcgtcggccc tgaccgacga ccagctgttc
      421 gagatcgcca cctcgctccg gcgagcgtcc gctgccgagg tcgaggcggt cttcgctccc
      481 ctcctggacg gctgacgtcg cggcgctcga accgactcga gcaccaccga ccgcttcagt
      541 gtcactggta cgtgcttgaa tggaggcatg acgaacacca gttcgccctt caccactcac
      601 accgctaccg ggcgcgccgt cgagaccgat cttcggcgag cgttgatcgc gctccaggtg
      661 gtcatggccg aactcgaccc cgccgcgatc ccactccccg acgccactgc cgtgttcacc
      721 ctgttcgccg acgttcgccg cctcgccggt aacggtcaga ccctcatggc cggccgggtg
      781 aaggaggccc gggagtgggc gcgttcgggc cacgcgaccg tcgaggactg gatcgccaac
      841 accaccggct cgtccgtcgg cgccgccaag gaccaggtac gcacctctga acggctcgac
      901 caactgcccg acaccaccga acagatgcgc accggtgggg tgtcccccga acaggccgac
      961 atcgtcgctg acgccgccgc ggtcaacccc gcctccgagg gcgatctcct cgagcgggcc
     1021 cgccgcgact ccaacaaggc actccgcgac gaggcggccc gccgcaaggc acaggcgagg
     1081 ggcgaatccg ccaacgagaa ggcacagcgg caccgcgccg agcgatcggc acggttctac
     1141 aacgaccgag acggccgccg ttgcctgtac gcgcagggca ccgccgccga cctcgccgca
     1201 ctcgaagctg cgatcgaccg ggccgtcgac cgccggtacc gcgacaccgg ctcgaccggc
     1261 cggcgcgagg gtcgggatcg ctacgcgttc gacgcgctca ccgccctggc cgacagcggc
     1321 accggtgatc ccaagcgggt gcaacccaag cacctcgccc tcatccgggt cgacctggcc
     1381 gcgctggtgc ggggcacggt cgacgagggc gaggtgtgcg agatcgccgg cgtcggcccc
     1441 atctccgtcg acgaggcccg gcgcctgttg ggcgattcga tcctccacct cgtctccgag
     1501 aacggcgtcg atgtccagaa cgtcacgcac ctcggccggg gccccaacgc cgtgcaacag
     1561 gtcgccctcc tgtggcgcac ccgacagtgc accgtgatgg gctgcaaccg cacccacacc
     1621 gagaacgacc accgcgtcga cttcgccgac acccggcaca cccgcgtcga cgagatcgat
     1681 cagatgtgcg atcaccatca cgacctcaag acctaccggg actgggtgct cgtccccggc
     1741 accggccgac gcgagatgat cggcccccac gatcctcggc accc
//
LOCUS       DAOSOA010000434         1568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_79503_length_1568_cov_4.241904, whole genome shotgun sequence.
ACCESSION   DAOSOA010000434 DAOSOA010000000
VERSION     DAOSOA010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79503_length_1568_cov_4.241904"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKY13_16850"
     CDS             complement(<1..751)
                     /locus_tag="PKY13_16850"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOT81664.1"
                     /translation="MSVLVPTTLEAALLALAADPDAHLLAGGTDLMVEVNFGHRRPSS
                     VITVNRIDELRHWSIDLDAAAVRIGSAATYTELMGSEVAALVPALAEAARTVGSPQIR
                     NAGTIGGNVGTCSPAGDTLPVLTALGASVELASLAGTRTLPFVDFMVGPKRSARRPDE
                     IVTAVIVPIVDGWQGFAKVGTRNAMVIAVCNAALVVDRPAASVTVAMGSVGPTIVRAP
                     EASAYVQDKIDWDSMRLVGDADEVDDVVREFG"
     gene            complement(806..1336)
                     /locus_tag="PKY13_16855"
     CDS             complement(806..1336)
                     /locus_tag="PKY13_16855"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOT81665.1"
                     /translation="MPQSALIMEVPWAEPVVGPWRRAHDAISHRGVPAHITILYPFRA
                     PDVLDDTVNATLASLAASVPAWDLSLVEVDAFPDAVWLRPEPDDHLHAPADSVWAAFP
                     ACPPHGGQFPALRPHVTVGQDPDPERIDLLRRRAAEELATMLPVTGRVDGLSLFVSGE
                     AGEWTRCTVFPFASGA"
BASE COUNT          274 a          531 c          546 g          217 t
ORIGIN      
        1 cgccgaactc gcggaccacg tcgtcgacct cgtcggcgtc gcccacgagg cgcatcgaat
       61 cccagtcgat cttgtcctgg acatacgccg aggcctccgg cgcccgcacg atcgtcggcc
      121 cgaccgaccc catcgccacg gtcaccgacg ccgccggccg atcgacgacg agcgccgcgt
      181 tgcacaccgc gatgaccatc gcattgcggg tgccgacctt cgcgaacccc tgccagccgt
      241 cgacgatggg cacgatgacg gccgtcacga tctcgtcggg gcgacgggcg ctgcgcttcg
      301 ggcccaccat gaagtcgacg aacggcagcg tgcgggtgcc ggccaacgac gccagctcga
      361 ccgacgcacc gagcgcggtg agcaccggga gggtgtcgcc cgccggtgaa cacgtgccga
      421 cgttgccgcc gatggtgccg gcgttgcgga tctgcggcga gccgacggtg cgggccgcct
      481 cagccagcgc cggaacgagc gctgcgacct ccgaacccat gagctcggtg tacgtcgcag
      541 cggacccgat gcggacggcg gcagcgtcga gatcgatgct ccagtgtcgc aactcgtcga
      601 tgcggttgac ggtgatgacc gacgacgggc ggcggtggcc gaagttgacc tcgaccatga
      661 gatcggtgcc ccccgccaac aggtgggcat cgggatcggc cgccagcgcg agcaacgccg
      721 cttcgagggt tgtgggcacc agcacactca tcggttcgga cgttaccgcc tcgatcgccg
      781 cgatcccggt cccgaaacag acacttcacg ctccggatgc aaacgggaac acggtgcatc
      841 gggtccactc ccccgcctcg ccgctgacga acagggacag gccgtcgacc cgacccgtga
      901 cggggagcat ggtcgcgagt tcctcggctg cccgacgacg cagaagatcg attcgctcgg
      961 gatcggggtc ctgtccgacg gtgacgtgag gtcgcaacgc cgggaactga ccgccgtgtg
     1021 gtgggcaggc ggggaaggcc gcccacaccg aatcggcggg tgcgtggaga tgatcgtcgg
     1081 gctcggggcg gagccacacg gcgtcgggga acgcgtcgac ctcgaccaac gagaggtccc
     1141 atgccggcac ggacgcagcg agggaggcga gcgtggcgtt caccgtgtca tccagcacgt
     1201 caggtgcgcg gaatgggtac aggatcgtga tgtgcgcggg gacgccacgg tgcgagatcg
     1261 cgtcgtgcgc ccgacgccag gggccgacca cgggctcagc ccaggggacc tccatgatca
     1321 acgccgattg gggcatggcg agacggtact cccggggtcc tctcggacgt gggcagttgt
     1381 gccagatcag cgttgacctt cgtttgactg tcagaccctc caagtagact cgaacacatg
     1441 ttcgacgtgg cagtgctagc cgagatgacg gcgaagatcg ccggcctcga cgagcacctc
     1501 tacgccgacg acgacctgct ggccggcgtg aaagcgatcg aagaagcccg ccgtcatctc
     1561 gacgcagc
//
LOCUS       DAOSOA010000435         1564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_79785_length_1564_cov_3.705765, whole genome shotgun sequence.
ACCESSION   DAOSOA010000435 DAOSOA010000000
VERSION     DAOSOA010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79785_length_1564_cov_3.705765"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PKY13_16860"
     CDS             complement(<1..1077)
                     /locus_tag="PKY13_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT81666.1"
                     /translation="MAPSRTARSVALLCATLLLGSVLASCSGVAPSDADTRSVDGVTA
                     DARRRPGTTTKPTTTAAPATTATPTTATPTTVAPPTTGAPTTTPPTTAPVSASLCQRL
                     TDQARSNPARYAASTTFNRPAACLGVRADSATWSSKWFRFANYPGRTDASKRGHISVS
                     FDAYSTPVYSTEDATTTIKVFGAGWAYGVELGNNNTIPWNPTWEPAPGNDMEMVIVDP
                     TTSTEWGLWGVQKVNWTSCFTLENLLAGWRAGIDLCVWSAGIGRDVDGSVSNAVTSSG
                     YSKEAGRGMGAIQAMALLPTLDEVEKGSINHALNMETYGTMFGPACPASGGGTPGVDC
                     GYAVAPATRLEWWNGPASECGSVAQ"
     gene            complement(1221..>1564)
                     /locus_tag="PKY13_16865"
     CDS             complement(1221..>1564)
                     /locus_tag="PKY13_16865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT81667.1"
                     /translation="AGINDLTGGRSVTQMLAGVTEFANYAASIGAKVTWVGVVPLPQT
                     ATISNREADRRAFNTALAQRYGARYLDCSPQMSNAAGFLTPGLSMAPLDLHLNNAGEQ
                     ALADCINAFRH"
BASE COUNT          207 a          509 c          588 g          260 t
ORIGIN      
        1 ctgggccacc gagccgcact cgctggcggg accgttccac cactcgagcc gggtcgccgg
       61 tgcgacggcg tacccgcagt cgaccccagg ggtgcccccg cccgacgccg ggcacgccgg
      121 accgaacatg gtgccgtagg tctccatgtt cagtgcatgg ttgatggagc ccttctcgac
      181 ctcgtcgagc gtggggagca acgccatcgc ctggatggcg cccatgcccc gcccggcctc
      241 cttcgagtag ccgctcgagg tgacggcgtt cgagacggaa ccgtcgacgt ccctgccgat
      301 gcccgccgac cacacgcaga ggtcgatccc cgctcgccag ccggcgagca ggttctcgag
      361 cgtgaagcac gacgtccagt tcaccttctg gacgccccac aggccccact cggtgctcgt
      421 ggtcggatcg acgatgacca tctccatgtc gttgcccggt gcgggctccc aggtcgggtt
      481 ccacgggatc gtgttgttgt tgccgagctc cacgccgtag gcccacccgg cgccgaacac
      541 cttgatcgtc gtggtggcgt cctccgtcga gtacacgggc gtggagtagg cgtcgaacga
      601 gaccgagatg tggccgcgct tggaggcgtc ggtccggccg gggtagttcg cgaagcggaa
      661 ccacttcgac gaccaggtcg ccgagtcggc tcgcaccccg aggcaggccg cgggtcggtt
      721 gaacgtcgtc gaggcggcgt agcgggccgg gttcgagcga gcctggtcgg tgagtcgttg
      781 gcagagggag gccgacaccg gcgcggtcgt cggcggggtc gtcgtcggag cgcccgtggt
      841 gggcggggcg accgtggtcg gcgtcgctgt ggtcggcgtc gccgttgtgg ccggggccgc
      901 ggtggtcgtc ggcttcgtcg tcgttccggg tcggcgccgg gcatcggcgg tgaccccgtc
      961 gacgcttcgg gtgtcggcgt ctgacggcgc aaccccagag cagctcgcga gcacactgcc
     1021 gagcagaagg gttgcacaca ggagcgcgac ggatcgcgcc gttcgtgaag gagccatcgg
     1081 gttccgttcc tcttcaggtg acggaatccc cagcggcttc cgtcacgtga ggtgtcgggc
     1141 cgatcgttgc ggaccttgag ttctgtcggc cgacatcgtc ggtcgatccc cccggcgcgc
     1201 cggcgggagg gtgacgggcg tcagtgtcgg aacgcgttga tgcagtcggc gagcgcctgc
     1261 tcgccggcgt tgttgaggtg caggtcgagc ggcgccatcg acaggccggg cgtcaggaac
     1321 ccggcggcgt tcgacatctg cggtgagcag tcgaggtacc gcgctccgta ccgctgggcg
     1381 agggccgtgt tgaaggcccg tcggtccgcc tcgcggttgg agatcgtggc ggtctggggg
     1441 agcggtacca ccccgaccca cgtgaccttg gcgccgatgg aggcggcgta gttggcgaac
     1501 tcggtgactc cggcgagcat ctgggtgacc gaacggccac cggtgaggtc gttgatgccg
     1561 gcca
//
LOCUS       DAOSOA010000436         1429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_90818_length_1429_cov_7.547307, whole genome shotgun sequence.
ACCESSION   DAOSOA010000436 DAOSOA010000000
VERSION     DAOSOA010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90818_length_1429_cov_7.547307"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1429
                     /gene="purL"
                     /locus_tag="PKY13_16870"
     CDS             <1..>1429
                     /gene="purL"
                     /locus_tag="PKY13_16870"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOT81668.1"
                     /translation="PLRFGPLDDARSRWIAEGVVSGVSGYGNSVGVPTIGGEVVFDET
                     YQDNPLVNVFCAGLLPHDRLVLGQASGVGNLAVLLGSTTGRDGIGGVSVLASAGFSDD
                     ETDAAKRPSVQVGDPYEEKRLIEACLALLDAGYVVGIQDLGGAGLACATSETASRGGV
                     GMDVYVRAVPQREAGMEPYEIMTSESQERMLAIVEPANLADVLAICERWEVLASVIGT
                     VTEGGALRLLDCDGPESASDDVVVGDLPAASLHESAPLYHRPLAPPADLDARRADSAN
                     ALDAGDLAADLLAMLCDTTWVSSQYDHQLFLNTVEEPGGDAAVLRLKHPLTGFDTGRG
                     LAFTTDGNHRWCAVDPRVGATATVVESVMNLATVGARPLALVNCLNFGNPEHPEVMWQ
                     LSEAIDGMSEACRAFDLPVIGGNVSLYNESRGSDIDPTPVVATLGIVDSLDRRPPGVG
                     LVEGGRLLLLGSTERELSGSLWARNR"
BASE COUNT          190 a          511 c          493 g          235 t
ORIGIN      
        1 acccactccg cttcggtccg ctcgacgacg cccggagtcg gtggatcgcc gagggtgtcg
       61 tgagcggcgt ctcgggctac ggcaactccg tgggcgtccc gaccatcggt ggcgaggtcg
      121 tcttcgacga gacctaccag gacaaccccc tcgtcaacgt gttctgcgcc ggcctcctcc
      181 cccacgaccg actggtgctc ggccaggcgt cgggtgtcgg caacctcgcc gtcctcctcg
      241 ggtccaccac cggtcgtgac ggcatcggtg gcgtgtcggt cctcgcgtcc gccggcttca
      301 gcgacgacga gaccgatgcc gccaagcgcc cgtcggtcca ggtcggcgac ccctacgagg
      361 agaagcggct catcgaggcg tgcctcgcgc tgctcgacgc cggctatgtg gtcggcatcc
      421 aggatctcgg cggcgccggg ctcgcgtgcg ccacgtcgga gacggcatcg cgcggcggtg
      481 tgggcatgga cgtctacgtc cgggccgtgc cacagcgtga ggcgggcatg gagccctacg
      541 agatcatgac gtcggagtcc caggagcgga tgctcgcgat cgtcgagccg gccaacctcg
      601 ccgatgtgct ggcgatctgt gagcgctggg aggtgctggc ctcggtgatc ggcaccgtga
      661 ccgagggcgg cgcgttgcgg ctcctcgatt gcgacggccc cgaatccgcc tccgacgacg
      721 tggtggtcgg cgatctgccg gccgcgtcgt tgcacgagtc ggcgccgctc taccaccgtc
      781 cgctcgctcc gccggccgac ctcgacgccc gccgcgccga ttccgcgaac gcgctcgacg
      841 ccggcgatct cgcggccgat ctgttggcga tgctctgcga cacgacctgg gtctcgtcgc
      901 agtacgacca ccagctgttc ctcaacaccg tggaggaacc cggcggcgac gccgcagtgc
      961 ttcggctgaa gcaccccctc accgggttcg acaccggcag gggcctggcg ttcacgaccg
     1021 acggcaacca ccgttggtgc gcggtcgatc ctcgtgtggg tgccactgcc accgttgtcg
     1081 agtcggtcat gaacctggcg acggtcggcg cccgccccct cgccctcgtc aactgcctca
     1141 acttcggcaa ccccgagcac cccgaggtga tgtggcagct gtcggaggcg atcgacggga
     1201 tgagcgaggc ctgtcgggcc ttcgacctgc cggtgatcgg tggcaacgtc agcctctaca
     1261 acgagtcccg cggcagcgac atcgatccga cgccggtggt cgcgacgctc ggaatcgtcg
     1321 actccctcga tcgtcgtccc cctggcgtcg ggctggtcga gggtggtcgg ctgctgctgc
     1381 tcggcagcac cgaacgggag ttgtcgggct cgctgtgggc gcgcaaccg
//
LOCUS       DAOSOA010000437         1290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_105750_length_1290_cov_5.420243, whole genome shotgun
            sequence.
ACCESSION   DAOSOA010000437 DAOSOA010000000
VERSION     DAOSOA010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105750_length_1290_cov_5.420243"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..>1290
                     /gene="pheS"
                     /locus_tag="PKY13_16875"
     CDS             332..>1290
                     /gene="pheS"
                     /locus_tag="PKY13_16875"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOT81669.1"
                     /translation="MTDTDPLSTTATIVADGLALISSVGTLDALRATDHDLLGKTAPL
                     SQLKASMRDVAPDQRKALGQALNDARRELETAIAERQAHFEAAARREQLAAEQLDLSE
                     MIVDRTVGHHHLITQTRERLEDAFIGLGFTIAEGPEVETDWYNFEALNMPAGHPARSM
                     WDTLYLDAGEPETILLRTHTSPVQVRTMMRQEPPIYIVAPGRTFRSDTADATHLPVFH
                     QIEGLVIDKGITFADLAGTIEAFVKVLYGEDRTTRLRPSFFPFTEPSAEFDMTTPSGD
                     WVELGGCGMVHPNVLRHCNLDPEEWSGFAFGFGIERMAAQRHGL"
BASE COUNT          223 a          464 c          416 g          187 t
ORIGIN      
        1 tgagccgttg cagtcgtgcc cccggttccg agacggaacc ccgtgccgac ggtgaccggt
       61 cgctcgaacc acggaatatc ggcgggcgcc gcgtccaccg gcgctgtccg ggcgatactc
      121 ccggcaacga cggccgacgt gagcacggcc gcggccgcga gcttgatccg attcatgtcc
      181 actccccatc ccacatctgc cccagggcca gaggcatcgc catggtcgca aagtgcgtga
      241 ggtacagcaa gatgacatct gcccaacggc gaagttcgtc ggacgacgaa tgagttcgga
      301 ggaggggagg atcgtccgcg agaatgtaca gatgaccgac accgacccgc tgtcgacgac
      361 cgcgaccatc gtcgccgacg gcctcgcgct catctcgtcg gtgggcaccc tcgacgcact
      421 gcgcgccacc gaccacgacc tgctcggcaa gacggccccc ctctcgcagc tgaaggcgtc
      481 gatgcgcgac gtcgctcccg accagcgcaa ggcgctgggc caggcgctca acgacgcccg
      541 ccgcgaactc gagaccgcga tcgccgagcg ccaggcgcac ttcgaggccg cagcacgccg
      601 cgagcagctc gccgccgagc aactcgacct gtcggagatg atcgtcgacc gcaccgtcgg
      661 tcaccaccac ctcatcaccc agacccgcga gcggctcgag gatgcgttca tcgggctcgg
      721 tttcacgatc gccgagggtc ccgaggtcga gaccgactgg tacaacttcg aggcgctcaa
      781 catgcccgcc ggtcacccgg cccggagcat gtgggacacg ctgtacctcg acgccggcga
      841 acccgagacg atcctgctcc gcacgcacac gtcgccggtc caggtgcgca cgatgatgcg
      901 tcaggaaccg ccgatctaca tcgtcgcccc ggggcgcacg ttccggagtg atacggccga
      961 cgcgacgcac ctcccggtgt tccaccagat cgaggggctg gtgatcgaca agggcatcac
     1021 gttcgccgat ctcgccggca ccatcgaggc gttcgtgaag gtgctgtacg gcgaggaccg
     1081 cacgacgcgc ctccgtccgt cgttcttccc gttcaccgag ccctcggccg agttcgacat
     1141 gacgacgccg tcgggcgact gggtcgagct gggtgggtgc ggcatggtgc accccaacgt
     1201 gttgcgccac tgcaacctcg atcccgagga gtggtcgggg ttcgcgttcg ggttcggcat
     1261 cgagcggatg gccgcccagc gccacgggct
//
LOCUS       DAOSOA010000438         1037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2357
            NODE_147689_length_1037_cov_6.298371, whole genome shotgun
            sequence.
ACCESSION   DAOSOA010000438 DAOSOA010000000
VERSION     DAOSOA010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864424
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,377
            CDSs (total)                      :: 3,350
            Genes (coding)                    :: 3,342
            CDSs (with protein)               :: 3,342
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147689_length_1037_cov_6.298371"
                     /isolate="AMR_MDS_2357"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKY13_16880"
     CDS             <1..746
                     /locus_tag="PKY13_16880"
                     /inference="COORDINATES: protein motif:HMM:NF023367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT81670.1"
                     /translation="RLIERFEAEFPDPLQPTYVLENHDRTRSLSRVGGDLAKARVLAT
                     MLCTLRGVPVVYQGQEIGMTNRYIPLREADDPIPGLLARRLPEAVNRRLPERLNRDEV
                     RTPMQWTAAPGVGFCPDDVAPWLPVNDNRSERNVADQSGDPSSLLSLLSLYRTLLALR
                     AASPALSGGSLHLVDSEADEVLAYERSDGEERLLVVANLGARGAEHTITGGADIVVAT
                     DPSITVRVAGGGRSTVRLAPNSAVVLRPA"
     gene            complement(733..>1037)
                     /locus_tag="PKY13_16885"
     CDS             complement(733..>1037)
                     /locus_tag="PKY13_16885"
                     /inference="COORDINATES: protein motif:HMM:NF020348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HOT81671.1"
                     /translation="SDTGLPSHAELGYLRSDGTSAVELILAHPTGLTELCEGTIDGPT
                     ITLRSISVVGSSTAKPVRAVERHLTVEGDRLHYVLRMSAVGLPMTHHLEAELVRQA"
BASE COUNT          154 a          368 c          359 g          156 t
ORIGIN      
        1 ggcgcctgat cgaacggttc gaggccgagt tccccgatcc gctccaaccg acctatgtgt
       61 tggagaacca cgaccgcacc cgctcgctgt cccgagtcgg cggagacctg gcaaaggccc
      121 gggtgctggc gacgatgctc tgcacgctcc gcggcgtgcc cgtcgtgtac cagggccagg
      181 agatcgggat gaccaaccgc tacatcccgc ttcgcgaggc cgacgatccc attcccggac
      241 tcttggcgcg ccgcctcccc gaggcggtca accgccgcct acccgaacgc ctcaaccgcg
      301 acgaggtgcg gacgccgatg cagtggaccg ctgcgcccgg cgtcgggttc tgccccgacg
      361 acgtcgcccc gtggcttccg gtgaacgaca accgctccga gcggaacgtg gccgatcaat
      421 cgggcgaccc gtcgtcgctg ctctcgctgc tctcgctgta ccgcacgctg ctcgcgctgc
      481 gagcggcgag cccggccctc tccggtggct cgctccacct cgtcgacagc gaggccgacg
      541 aggtgctcgc ctacgaacgt tccgacggcg aggagcgact gctggtcgtc gcgaacctcg
      601 gcgctcgtgg agccgaacac accatcacgg gcggcgccga catcgtggtc gccaccgacc
      661 cctcgatcac ggtgcgagtc gccggcggtg gccggtcgac ggtgcggctg gcgccgaact
      721 ccgccgtggt gctcaggcct gcctgaccag ctcggcttcg aggtggtggg tcatgggaag
      781 tccgacggcg gacatccgca gcacgtaatg gagacggtca ccctcgacgg tgaggtgacg
      841 ttcgacggcc cgcaccggct tggcggtcga cgaaccgacg accgagatcg accggagcgt
      901 gatcgtcggg ccgtcgatcg tgccttcgca cagttcggtg aggccggtcg ggtgggcgag
      961 gatcagctcg accgccgacg tcccgtcgga acggaggtat ccgagttcgg cgtgggaggg
     1021 cagcccggtg tcggacc
//
